LOCUS       DAOQOS010000001       126903 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_37_length_126903_cov_6.457319, whole genome shotgun sequence.
ACCESSION   DAOQOS010000001 DAOQOS010000000
VERSION     DAOQOS010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 126903)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 126903)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..126903
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37_length_126903_cov_6.457319"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(632..1501)
                     /locus_tag="PLF66_00005"
     CDS             complement(632..1501)
                     /locus_tag="PLF66_00005"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HMZ62440.1"
                     /translation="MKFSILKKVLIGFLVVIGCFCAFVMIRFEMEKSKTYSSLDFEIT
                     TEVEKADLELGKRIYHVRAGCVDCHGKDLSGAKIMENGAMGVIYGANISPFNLKNWSD
                     KEIARAIRYGVHKTGRSLRFMPSFDYQSLSKGDIAAVVKYIRSQPEVTKPSHENTFGP
                     VAKILSALNKIPVLFPAKMIDQQKGFADKPAEEVGIQFGEYLANSCVGCHGMDYKGGP
                     IPGADPSWPPASNIRLGANSSYSLDSFREMIKTGVSPSSKQELKFPMPIWLLKEFNDK
                     EIESLWIYLSSLK"
     gene            3026..3172
                     /locus_tag="PLF66_00010"
     CDS             3026..3172
                     /locus_tag="PLF66_00010"
                     /inference="COORDINATES: protein motif:HMM:NF019243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1574 family protein"
                     /protein_id="HMZ62441.1"
                     /translation="MGDDYGSSWIQIPRKKFRMPLFNMNEDPEYTCDYFTDASHMSTK
                     CFPD"
     gene            3621..3980
                     /locus_tag="PLF66_00015"
     CDS             3621..3980
                     /locus_tag="PLF66_00015"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HMZ62442.1"
                     /translation="MSNEQIVIDILQEGSVAIIYVRGDLTLLDAPQFKKIVDDEVNKG
                     IKKLLFDFENLTFLDSSGIGLLLRFHAQLRKLNGKIAYINFHKNVENTMQKALPQQIR
                     STSFFESKEKAMEYMNS"
     gene            complement(4094..5581)
                     /gene="glpK"
                     /locus_tag="PLF66_00020"
     CDS             complement(4094..5581)
                     /gene="glpK"
                     /locus_tag="PLF66_00020"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004783313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HMZ62443.1"
                     /translation="MKNYIIGIDAGTTGIRTFCFDTKGKVISVAYEEFKQIFPKPGWV
                     EHNATEIWQKTEKLILQAIKKGKLNPKNAICIGITNQRETTVLFEKKSGKPIYNAIVW
                     QCRRTSDICTDLKAKGLESDFRNRTGLVLDAYFSGTKIKWILDNVNGARDKANKGDLL
                     FGTIDTWLLYKLTKGKSHKTDHTNASRTLIYNIKDKKWDVDLLNILNIPASILPETHT
                     SRYEFGKTEGVKGLPDGIPITALVGDQQGALFGQLCTEVGEAKNTYGTGCFLLFNIGN
                     DFQISKNGLITTLACGPEGNTVYALEGSVFIGGAVIQWLRDYLKFFKDSKDTFKIVKT
                     LKNEDDIVFVPAFAGLGAPHWDMNARGAIYGITRDTNQAQIVRAALKSIALQSYELVS
                     AMENDTGKTLKILKVDGGATTNEYLMQFQADIIGKKVQRPDNVDTTVLGAAYLAGLES
                     KVFSSVESLKSMNKKFKEFKPTMKAEQREKEIQNWNKAIVKTKTV"
     gene            complement(5701..7131)
                     /locus_tag="PLF66_00025"
     CDS             complement(5701..7131)
                     /locus_tag="PLF66_00025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62444.1"
                     /translation="MRIFNIFRIVIIFSLSVTVSLQAQKTKKGKQKQKPSIKTSISKE
                     KSQIAEKNEREEVKNISVKAEEPLAMEEKTNMVESEKQEPSVLKEEPKTSPSPKSEVV
                     NPASTTPAKEESVNPITFNQYFSNDIWFRGFSVLGNKLSQRDNMAYDSIPQAWNVVTG
                     VAYNPTENISIGMNVYSPTAHRANRDNDYFMQTASGDKTDFSQKYLESALAGNPNILI
                     NDALTRDKANPKDPSSIRMRKEKNGLKDIFDASFTYKYNTRFGKVITGAYIANNDNYN
                     VTLGELVTGLEFPFLKAISPTYTSYYRVTSEGGGGGNGTSNHRLSISHKFFPDKELNF
                     TASLAAGYQYHNNLSDYRSGVSDITPKLQVNYGGMYFSVLDMIRPDSSLWDTTTGFGS
                     AGVYKDTNHRDGRVEDPSKVHGIQNQIVVDSITRAVDNYSAANSGADPNGYGREAIKA
                     HLIQNYQQQKFVHHIFHFTVGYSLKF"
     gene            7402..9144
                     /locus_tag="PLF66_00030"
     CDS             7402..9144
                     /locus_tag="PLF66_00030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62445.1"
                     /translation="MKTPNIILPIFYLLLIFLFPMCKEKKEELIFESKDKYDAAILNW
                     VEIFQPSTLNKQERLNELLWFKNVSKEFRGKKIKTVAENIDTHYWESRVLTKAFYDIT
                     GIEVEHEIIGEGDVVVRIMDQIENGTQWYDAYVNDADMVGTHLRTQGVVVLSDYMKKE
                     GKDFTNPNLDLDDFLNLEFGQDYDGNQLQLPDQQFANLYWFRYDWFTREDIKKQFKQK
                     YGYELGVPINWAAYEDIAEFFTNTPIDGKKVYGHSDYGQPGASLGWRFTDAWLSIAGV
                     GDKGLPNGLPVDEWGIRVENKVPVGSSVSRGGELNGPAAVYALTKYIDWMKKYARPDS
                     FELDWATHGPTASEGNVAQQIFMYITWLSDARFHSADSPVCDKYGKPKWRVAPTPHGK
                     YWQEGMKVGYQDAGSWTIPKNVPSDRKKMAWLWAQFAVSKTVDLKKFSIGGTPIRKST
                     IHSEYLSKRLEDYGGLIEFYRSRDEKTWTDSGPNVPYYSGLSALWWKNVAKAITGEFT
                     PQEAMDNLAEEQDELMSKTKLNAYAPKLNPKKDPSYWFAQPGSPRPEIKERETPKTIA
                     YEELLKQWTKEGKK"
     gene            9141..11429
                     /locus_tag="PLF66_00035"
     CDS             9141..11429
                     /locus_tag="PLF66_00035"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HMZ62446.1"
                     /translation="MNTRTNFEKVFTFHRFTKLSLRKKVNFIGIFIILLFSVILFVKV
                     LPLLEAGKLEERRGKLRAVVNSVVSLIDHYERGLRKQSWKTDPTQPKNIEEAKNRIIE
                     NLKYMRYDKTEYFFILDGKGKMVMHPLKPELEGQDMMNTKGPKGEAIFHDLVLQAQRD
                     SEAFVSYTWQSKYSPLIFEPQTSYGKYYWAWDWIVCSGVYTQDILDSMQEINYLSAGY
                     VVVTSAFTIFILFIFVYFNLSKPLMNLLKGIEEIKKDNLDHIVEAHFDDELGHITRQF
                     NAMTENRKRSQEELIQLNASLETKVIQRTAELSETLKQVQELKVKQDGDYFLTSLLLS
                     PLQSKSAEEENVKSEFFIKQYKTFSFRNWNAELGGDICITDSIKLNGRKYLFFVNADA
                     MGKSIQGAGGALIFGTSVKAALIRIKLGKSPNILPEAWLKNLYHDLDELFTTFDGSML
                     ISAVMGLIDEGNGLVYFLNAEHPWPVLYRDGKASFIEGQNQLMKIGTPEKEYNLLQVN
                     IFQLKDDDTFIAGSDGRDDIILIGTDDVNHDETLFLKIVEKAQGDINEIVEGISESGK
                     LKDDLSILSIKFNKLSTENNFDLSTEEKKILLKATKLHKNNENEEALALLKRNFDHKH
                     FSIPVFNLIKRIYLVQKDYLQYSKLLIRYHMLHPEKNENLLKISKALVLCNQLDQAAD
                     YAECLRVREPHNEDNLLHLAKIYKLSSHYNVALRRLNEVLELNPSNEEAFKLKENIEL
                     LIHKANIINLDESLFEISKFDY"
     gene            12160..12981
                     /locus_tag="PLF66_00040"
     CDS             12160..12981
                     /locus_tag="PLF66_00040"
                     /inference="COORDINATES: protein motif:HMM:NF018091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HMZ62447.1"
                     /translation="MDTVNFETAEEHWIEIDGLKIHYREAGEGKCLVLIHAAGHSLED
                     FNSIFDKLKTSYRVIAIDLPGHGKSDLFDPPTFANVITLSKYIQEFSKQVVKEKAIYL
                     GNSIGGFLSISLALASPELVKGLVLVNSGGFNEPGLIADNFTRLKGKEWITRIVWNTF
                     PSLYIKVKNEFTKDILSRIRNNKTDNAIKTNASIWRSFLLPEHNLRERAKEIECPTFL
                     IWGKRDPVIELKIGIQATQLIPDARLELLNTGHLPFAEDPENFFTVLKSFLETIE"
     gene            12978..13514
                     /locus_tag="PLF66_00045"
     CDS             12978..13514
                     /locus_tag="PLF66_00045"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HMZ62448.1"
                     /translation="MKTSGPKTKEALIQSALTLFEKKGISNVSVDTITKAANVAKGSF
                     YTHFKNRADFLIYLHRDFHEKLKLEITKGIQNQPFGMERLWIGTETYLNYCLSNSSMK
                     SFLFEARIEPALTKEVSNRNREFSIWAEKDFAKAGWEYTQATASLYVGMIAETAIQEM
                     KAKKKLQNLRNAIAQFID"
     gene            complement(13583..14065)
                     /locus_tag="PLF66_00050"
     CDS             complement(13583..14065)
                     /locus_tag="PLF66_00050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62449.1"
                     /translation="MKQSKKISILAMALVILWNCKDNSLELKFKKDHEVLEAKHTSME
                     ADHAKLEADHEAFIKKHNESKGKGDDKAHEEKEVLHGKILEAHRAVIEKHKKLIDEHA
                     ELEKKHLSGSLSKEDVEKDHQRILKEDESMNAEHEKLIEEHKSLLGEDNQMLEEHKKK
                     "
     gene            14402..14851
                     /locus_tag="PLF66_00055"
     CDS             14402..14851
                     /locus_tag="PLF66_00055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62450.1"
                     /translation="MSDKLNSRLSTIRILHITLIMPPIVLYGFFFINQLIFIEDIVSK
                     IVGILIAASSVISYEFFLKQSFISRYKSKTFEEKLIGYQSLKILQYSMIEGAAVFNVI
                     LAAGEPNPMNAPIVALLVLYYILFLRPSKSEFLKLFQLKEHDLLNTQ"
     gene            14925..16043
                     /locus_tag="PLF66_00060"
     CDS             14925..16043
                     /locus_tag="PLF66_00060"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HMZ62451.1"
                     /translation="MSLISFIENSFLIAGGFFGVIWGLQILITKKYYSLSLVLFISSL
                     WLFAGAYSFSGIYLQYPNLFLLHLPFVYCVGPLLYFFYKKNLMDEEWKTFYFHFIPAI
                     LVFLAILPFHFYDTDQKYLFLIGIKEQKNTIYSLLILFLNFGTKVHPLIYLTRIILEN
                     KTFFKEQSSNLLYFIIICSSIYLDLCIGILGFLFKSFFLIKLSAWLLPINLCIFFLLA
                     SKYPDMLEMVKTEFKKSKYRNSKIQTLNLESVLSNLEQLMNQEKAFSDEDISLTSLAN
                     ELSITPHQLSEILNEKLNKNFAQYINEFRIAEAKKLLLEESKRSILSIGYAVGFNSKA
                     SFNRVFKITTGVTPAEFRKNYKVSNYNLTSNHKSHSIN"
     gene            16097..17968
                     /locus_tag="PLF66_00065"
     CDS             16097..17968
                     /locus_tag="PLF66_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012388625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HMZ62452.1"
                     /translation="MFKNLVEMIYYSANRFGNQGAFATRNKSKKFDSVTYSELLEMSE
                     NLALALIEIGVQAREHVGIIADNRVEWIVSDIAIQMTGAADVPRGTDVTDNDILYILP
                     HADVKVLFVENNQTLEKVLKLKNKLGLTKIILMDKDAKLSEGIVNIYDLIERGKILRK
                     TKFYLLQERIQGIKEEDLFTLIYTSGTTGAPKGVMLTHKNMVFQINHLPISLSHSERI
                     LSILPVWHIFERVFEMIAIATGSCTYYTNIRNLKEDLQIVKPTFMASAPRLWESIYQG
                     IMGNLDKAPKSKKNLFNLAYTVSRVYHDSLRFLLKQKIQLEPSNHLFYFFMSIYHLSV
                     LFMLFIPNLILDKIVLSKIRQATGGQLRGSVSGGGALPTHIDEFFNNIGIPVLEGYGM
                     TETAPIISVRTFQNLIIGTVGKVFPQTSIRILDINSGKEIFPANKGYAIKGEIHVKGD
                     QVMRGYYKNPEATSKVLKDGWMNTGDLGVMTYNGCLKIVGRSKETIVLLSGENVEPVP
                     IEHRLLHSEFIDQCMVIGQDKKFLGALIVPNLDRFKEYGQDYKSIAENKYVYQKLSEE
                     IKKAISAENGFKSFEKVLDFRLLPKQFEVGDELTAKLSVKRHVIAEKYQHLIDSIYK"
     gene            complement(17970..18341)
                     /locus_tag="PLF66_00070"
     CDS             complement(17970..18341)
                     /locus_tag="PLF66_00070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62453.1"
                     /translation="MKSIFLIFLFLIACTPDGPTYIPESDARARLYLLTILKCSSSGG
                     DPRAKSLDIFSFVEKQFKTNTLAESDRKKVIYYKKDYQKCETLISILPIDKCDFKGID
                     LINLASKGGICKLEPASYYNF"
     gene            complement(18359..18961)
                     /locus_tag="PLF66_00075"
     CDS             complement(18359..18961)
                     /locus_tag="PLF66_00075"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016759057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HMZ62454.1"
                     /translation="MKIVKVFLFIPSFVLFTHCFCFLKERTKEEIYQKIFQENPVFIS
                     DGFDFPVGAPNAKKYYDAQPFGKNSHLGEDWNRVDGMDLGEPVYASSNGIVSFVQDIG
                     GGWGNVVRIVHCMPDGKLYETLYAHFQTVIVKPNAMVLRGQKIGTIGDAGGKYSPHLH
                     FELREDIHLPVGHGYSVLTAGYLNPKEFIKKNRKLEVKSK"
     gene            complement(18963..19520)
                     /locus_tag="PLF66_00080"
     CDS             complement(18963..19520)
                     /locus_tag="PLF66_00080"
                     /inference="COORDINATES: protein motif:HMM:NF019896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase family
                     metallohydrolase"
                     /protein_id="HMZ62455.1"
                     /translation="MIRYQIAIVILSIFLFCYKGKPSIPTRADWNHFSKDNWNEIDMV
                     HKYIMKEMPNNVFTIEEILRQGTEWKNLDEPPFVIPPKHLWKNILPTIYFINRFIIPK
                     IGEVEIVSSYRTRVYNQLAGGAKNSRHLTFNAFDMIPKKKISREQLHTILIQIWEKEG
                     RQYKVGLGLYSGLRFHIDTSGFRKW"
     gene            19728..20801
                     /locus_tag="PLF66_00085"
     CDS             19728..20801
                     /locus_tag="PLF66_00085"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HMZ62456.1"
                     /translation="MILEWKSYINRYTFYGSLFGLLFPFFSTILQSYSFFQSIDIELL
                     LLTQKNTPLLWIIDTAPFFLGLLAGYAGGKQITIKNALAELMRINREMKIEIIRSNTM
                     ETNLQDMISTYKQDLHSAKLVQEFSLPKIPKMNECNISYKYIPLNPVGGDFLSIVKFP
                     DGKLGLLIGDVVGHGISAALIAALAYALSYRAREYYSLSPKSYLEYLNNEAEQYLGED
                     YYFTALYGFIEFQNNKAFLTFSRGGHPYPFIYRKEKRITEIYQMPGSPLGLMRNLSYE
                     QLTIELNSGDKIFWITDGFIEIRNVQEKILGNDGLAEIIKESCEENQDIHQIIDGIIS
                     RSEAFANGAAYNDDRLILGIDIL"
     gene            complement(20804..21376)
                     /locus_tag="PLF66_00090"
     CDS             complement(20804..21376)
                     /locus_tag="PLF66_00090"
                     /inference="COORDINATES: protein motif:HMM:NF013989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HMZ62457.1"
                     /translation="MRKIILYMHMSLDGVVSDLEKWASIQDDTLYDSMERYDTVDTVL
                     FGGNTFNPMAEYWKNAELNSSDPLEKDFAKQINSKKKFALINRDLHIDWNASEKLLYT
                     SIEDLKSKLNELKAKEGKNISVESGIRTWHLFLENSLFDEIWLRIQPVIAGSGTRLFD
                     SVKNKMNLEVVESKQFPSGILGVTYRRTKN"
     gene            complement(21391..21966)
                     /locus_tag="PLF66_00095"
     CDS             complement(21391..21966)
                     /locus_tag="PLF66_00095"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HMZ62458.1"
                     /translation="MKLFLFFSLLSISIFADGENLKSAYVDYDAFEKIVAEVKEHRKN
                     RLVSLEKFLEMSKEDKVIILDTRSPAMYKSKHIKGAVHLNFSDFTQRNLARVIPSFDT
                     KVLIYCNNNFEDDQVNFASKIAAPPVANNTTPITLALNIPTYINLYGYGYKNVYELKE
                     LVSVRDERISFEGTSVLSLPQTESKTKSKSK"
     gene            22080..22637
                     /locus_tag="PLF66_00100"
     CDS             22080..22637
                     /locus_tag="PLF66_00100"
                     /inference="COORDINATES: protein motif:HMM:NF017496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HMZ62459.1"
                     /translation="MIVYDFGAEKKESTYADYLKTPEGGKFQLIGGEIIEITSPSLYH
                     QDILLNLSSLFRNFLLKNKIGKVFVSPLDVMLSEKDVYQPDIRILLNESFPKMKENKI
                     EGTPDLVVEILSPSTAYYDLKQKKTTYEKSGVREYWVVDPIQKTVELFENQNGKFISF
                     AEHSKTEIAKSRLVAGMEIELEQVF"
     gene            22684..23643
                     /locus_tag="PLF66_00105"
     CDS             22684..23643
                     /locus_tag="PLF66_00105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62460.1"
                     /translation="MRNYIKLFFQIIFLLFFPNSVFSDPYYFINVPYGVFTTSFNSNN
                     NGSKSTEMTESFFIPSFSRVEIRETKKDETNLFSYDSALKIRYNNKIGFLPKSYIGTY
                     NVINKKESPDRSKYFEIISPYKNCDCNRYTGYIDSCFMEIRSKNSKLIQRIGNDKQKA
                     CRNHYWTRVKDWFNENDLLMDGYGDGDAGSGGTWSFAKEKVNWSTGKINELYTVNQLN
                     CKDSEAKDGYSILFQVESSKNFYILWKEKLYRVDAPIIRFGSFKFKLMEEMDAYDCKI
                     DFKKMKPIQESSITIYKEGKNSISYNNQGEFNFFYNGKEISQH"
     gene            23947..24399
                     /locus_tag="PLF66_00110"
     CDS             23947..24399
                     /locus_tag="PLF66_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HMZ62461.1"
                     /translation="MSETTSALSKDIVGKKMDRFDFTVERGKIKEFCIAIGETNPIYF
                     DIEAAKKAGYDDIPAPPTFQTAVQFWGYPKIWEDMTSFGIDIKRLLHLKEEYTYLKPL
                     YPGNVWAQSEVVDVKTGKMDMVTFRTTYHNSKNEPCIQAEMAIVIRPN"
     gene            24410..24826
                     /locus_tag="PLF66_00115"
     CDS             24410..24826
                     /locus_tag="PLF66_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008590909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HMZ62462.1"
                     /translation="MKIEFDKVEVGQEIPSLTLPAITHANLVRYAGASGDFNPIHNDK
                     DFAIANGLDGTIAHGMFVMALIGRACTNWVDQKQIKFFGVKFKAMTKLGSVLTCKGVI
                     KKKKEENGEKLITLTVEAVDQNGEVKAGGDLIVACA"
     gene            24837..25382
                     /locus_tag="PLF66_00120"
     CDS             24837..25382
                     /locus_tag="PLF66_00120"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HMZ62463.1"
                     /translation="MLYRLLLISLFFFSFTLSADVNEEFIEAVKDTNIEKVKILLKSK
                     VNLNATDSREMTPLLIASVDNNLELVKLLVEAGADVNRKQKETGKTALMYSSANGHVE
                     ILRYLLSRPGILINAKDKEGKTALVHAVFFARKDAVSLLLENKANPNSRTNSEESALT
                     FAIKGGRPEIVSMLRQAGARD"
     gene            25345..26316
                     /locus_tag="PLF66_00125"
     CDS             25345..26316
                     /locus_tag="PLF66_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002974643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HMZ62464.1"
                     /translation="MFQCSDKPEPETRMKDAFLKSISTVYRLAFLLSILIFINILFGP
                     LVRATDSGLACPDWPLCYGKVIPPPEFRIWMEVGHRIYSGLLGFVILGLSIVIFKNSE
                     LRKRFGLLAVTALVVLVNQVILGKLTVTKLLDPGTVNMHLLNAVLLFTLIVSITAKAN
                     YILKSETELVSFIQWNQIFTTRNLLLTITVILIFFQLFMGGRVSSNYAGLACPDWPTC
                     HGSWFPKMEGLVRIQMEHRFVAYIILILIVINFSISIFKSYDRRTRLFLKMALHTVGL
                     QIILGVSNVLFQLPILVTALHTGVGVAVFILNYTALFYRILPEDNSN"
     gene            26323..27198
                     /gene="cyoE"
                     /locus_tag="PLF66_00130"
     CDS             26323..27198
                     /gene="cyoE"
                     /locus_tag="PLF66_00130"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008399620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HMZ62465.1"
                     /translation="MSSILKYISIWNKLIKPRVTTLVLVTSLPGLYLGGDGHPTGFLV
                     AMTLIGTFFMSSASFILNQYIEIDRDSRMERTKNRPLPKGEIKPKTALTVGLFFIFLS
                     FGILYYFANLLTALCALAGLLLYIFLYTIYLKPRTDQNIVIGGISGCIGPLIGYAAVT
                     NSLPLSAWTLFGMIFLWTPAHFWALAIFLKEDYDSAEFPMMPVVRGLKKTVQAIFMYT
                     ILYSISCVGFYFVSDRVGTFYLYSTVALCLLMIYLSIRLMISESKKFARFFFFFSIFH
                     LYLVNFIILFDRKIV"
     gene            27272..27889
                     /locus_tag="PLF66_00135"
     CDS             27272..27889
                     /locus_tag="PLF66_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008595363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HMZ62466.1"
                     /translation="MQFKSYNEFVSSLLSCQKCKGMQSKPIVGSVKKAKIISIGQAPG
                     IHEEKHGKPFSYTAGKTLFKWFASIGIQEEIYRQQVNMSAVCRCFPGKAKSGDRKPNA
                     EEIENCSIYLKYEIQYHKPSLIIPIGKMAIDLLIGNSKYTLEKVIGNQYKTSLYKHEF
                     DWIPLPHPSGLNVWNNTQIGKKLILKSLQLIQSHPATYNLIQPST"
     gene            27973..29316
                     /gene="thrC"
                     /locus_tag="PLF66_00140"
     CDS             27973..29316
                     /gene="thrC"
                     /locus_tag="PLF66_00140"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020781637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HMZ62467.1"
                     /translation="MTFVTSKPKYSALFQCINPDCKCEYELQEIVYLCRKCGDLLQVK
                     HDLNELKKTSSEEWKTLFDDRLKDVRFPNQSGIWSKREWVLPDLRTENIVSSGEGFTH
                     LYEAKRLSKELGLSELYVKQCGVSHTGSFKDLGMTVLVSHVNQLIADGIKIKAVACAS
                     TGDTSAALASYAAKAGIPTIVFLPANKVSNAQLIQPVSNGAKVISLDTDFDGCMKIVQ
                     EVSKEAGIYLANSMNSLRIEGQKTISIEMTQQLGWDVPDWIVIPGGNLGNVSALGNGF
                     EMLQELGLINKLPRIILAQAHNANPLYLSYLKGYSEFSPVDAKKTLASAIQIGNPVSV
                     KKAIRTLQKFNGIVEEAEEHELADAAARGDLHGLYNDPHTGVALAALFKQIEKGNVRK
                     GERVIVISTANGLKFTEFKVQYHENQIAKINSKLVNQIINCKANISSVMDVVNKL"
     gene            29325..30062
                     /locus_tag="PLF66_00145"
     CDS             29325..30062
                     /locus_tag="PLF66_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010415610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62468.1"
                     /translation="MDNLNGTIPTNSDEHLLTEKFKSKVLHGVDSNKTVIFITYVLGE
                     LGEAKLKFIIKTILLKYNRIDLMDLLYTCAKELIVNSTKAAIKRLTFKEMGIDPSDDS
                     EYDKFMSSFKDNLTDKKFPHYRSKMKANGFFIKIKLFYNKDKIVMRIINNFPLLKKEE
                     ARIREKFMNAKKYDNLFEFYIEHGDNTEGAGMGITMVEILLAQSGFDRHLFTIFSSKV
                     KQETVAKVELPMHPDYVPMRYGFNGKE"
     gene            30102..31172
                     /locus_tag="PLF66_00150"
     CDS             30102..31172
                     /locus_tag="PLF66_00150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62469.1"
                     /translation="MASENESQNELITMALESVSSDENVNLVLEGKLMDALIVKASVF
                     VETRSGILLFLFSIEKGELIEIYSIFGNSPLINRIRTFENYSQFLIDLIETVKGGGIE
                     KALSDRIGRALYKGINPTNLLSLFPKMGKKNYNDFCMMMQEIMNNELKIQSTKMQIDI
                     EKVNSLKFRINNPLAEIYKPIQNPYKEVKKEPTKVVVPILENSPEKIKAEAMINDVQK
                     NYKQVLMCKTIVSPVAGIDFDSLKENMKLLFVLPFETPEEKALAKSVGAVSKEGTIKP
                     IAGEFLKIITGGKNEFHIFAKGPNGILLRAFEERPVKLALSKKNQQVAKKEERSELGK
                     MMSVVVGILFVLIIILSFMYLA"
     gene            31237..31575
                     /locus_tag="PLF66_00155"
     CDS             31237..31575
                     /locus_tag="PLF66_00155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62470.1"
                     /translation="MSSFIVLFFSSCKRNPQELKIKEAIRTGDYMTVAALCSAYQFPD
                     YKTECESSLEQADQEIQNIISRKQEMPFMKLILDPDKGIKIQELLKANIQMGIKYRSI
                     WNETAEIYKE"
     gene            31578..31994
                     /locus_tag="PLF66_00160"
     CDS             31578..31994
                     /locus_tag="PLF66_00160"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HMZ62471.1"
                     /translation="MVKQEDLKAILQKADLKITRSRIEVLKILYNTHKPITHTYIMEH
                     LPDKDVWDRVTVYRTLSEFAEKKIIKSLPSNERVTYFEIKNEAKPEHSHLVCDSCGKM
                     ECFDEDAYKFQFKKNIEDFRVRSLEIIIRGLCKNCQ"
     gene            31985..32266
                     /locus_tag="PLF66_00165"
     CDS             31985..32266
                     /locus_tag="PLF66_00165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62472.1"
                     /translation="MPVKVEKVIKIKKWTSTVFNSNEKRVKECLKEILPRYKTIHWDE
                     EKFIQFYIDVLNSLPCRYKQKNSIELTGKLKKQVLMDAICDKLDELSAS"
     gene            32266..33558
                     /locus_tag="PLF66_00170"
     CDS             32266..33558
                     /locus_tag="PLF66_00170"
                     /inference="COORDINATES: protein motif:HMM:NF013871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[cytidine(C)-cytidine(C)-adenosine (A)]-adding
                     enzyme"
                     /protein_id="HMZ62473.1"
                     /translation="MTQAKQLINSIPDTFKKDLEFISNEIQKKGKESFVIGGSVRDLI
                     LGKYPDEYDLTTSMQPDEVKKLFKRVIETGIKHGTVTVLIGDRSYEITTYRRDMDYTD
                     GRRPDRIEFGESLSEDMKRRDFTMNAIALDLINERLIDEHNGLEDIQRKIIRTIGDPL
                     KRFGEDGLRPIRAIRFLSTLGFTIEEETYLAILKTRNITEKISKERFHDELNKILISK
                     NPSIGLIELQKNKIFELFTKAKINHQNSADIEIIGKLELTPLGLRLAFMLDWLLETNE
                     KKENAEQFLRDIRYSRANLNDCLFYLELFKENPTLENGSDWENRRFAAKVVSYTGNRS
                     FDSYFHGIMSFLKLFNSETSVFKFQKEIESILHSTVPLSIKDLAVNGNQIIERFPNLD
                     KRKIGLILKHALEKTIELPDLNQTEKLFDLIETYLIEL"
     gene            33565..34917
                     /locus_tag="PLF66_00175"
     CDS             33565..34917
                     /locus_tag="PLF66_00175"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HMZ62474.1"
                     /translation="MKHIRPKFLEPLLPKIKSNYQDEFYRQMLVNEKKRVTIIASLIA
                     FILFVQIILRFFIPDSLINKYPTALEGSKYSIAILFLFFIYEIVVRFIFSNFIKKDIQ
                     PPEFIRYGNAFVETSVPTLGMILFGYFQEPTDALVSPILLVYFFFILLSTLRLNFKLS
                     LFTSFVAAAEYLGLCLYYYNTNQIKSLNMMSIELPLFYLRSCFFLIGGGISGLVAVQI
                     REGFLKSIETIEEKKQLMNVFGQHVSPEVVDKLLNQNVDIDSEIKHVCVMFLDIRNFT
                     SFSENKSPVEVVNFLNTLFAFMIEIVNQNNGIINKFLGDGFMAVFGAPISNGRDCYNA
                     VKASREILSMTKEYIRVSKIPPTKIGIGLHSGNAVTGNVGSELRKEYTVIGDVVNLAS
                     RIEQLTKQYEIEFLMTEDVFIQAMQIPNEMANNELDWREIDTVRVKGRITPVKIFTLV
                     "
     gene            34950..35663
                     /locus_tag="PLF66_00180"
     CDS             34950..35663
                     /locus_tag="PLF66_00180"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62475.1"
                     /translation="MKQMNQFTLKLFFISFLILITQCSLDNQMNYPTGVPKEASFDRK
                     KNVFVLRKDKHEYIWYDNGKLYSDCELNEIGIRNGKCEFFSEFNGNLLSKGNYVNNLR
                     DGLWHWYFPNGNIYYKQNFSHKFKRDFWVETNLIGNEHGPYERYYSNGQLEEKGEYDQ
                     GFKSGKWEKFYPSGKLEYTGAYEKDNKILSWKFYFPNGNLEAEENFSNKAEFLSRITY
                     FPNGNVNCKNVLNQPLDCK"
     gene            complement(35933..36316)
                     /locus_tag="PLF66_00185"
     CDS             complement(35933..36316)
                     /locus_tag="PLF66_00185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62476.1"
                     /translation="MSDTRSCGKHFLHVVPVFWFSIDNSPIVKYKGVKLHRTTKSGYY
                     MFASDLTIFEISFGVYYGFRLGINFDELLDFLFGIFGFDFKGDYGAKYFTFCIDYSKS
                     TEKELETRTKILDRIRTNTTSENTK"
     gene            complement(36405..36758)
                     /locus_tag="PLF66_00190"
     CDS             complement(36405..36758)
                     /locus_tag="PLF66_00190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62477.1"
                     /translation="MNLLIRFTMLACLLTISCSSVQTYFINRGNDALDIVNVGVEKNI
                     YGVRIKIFVPIGYQKANSGVGYGLRKGYLGKYFIGDPENKISIQNNEDEAKNLPVEYE
                     ILLGDTDFTSIKSHS"
     gene            36988..37344
                     /locus_tag="PLF66_00195"
     CDS             36988..37344
                     /locus_tag="PLF66_00195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62478.1"
                     /translation="MNALLENTIKELGFPSLEEFAKHQAIAILTQKILTYQKEIEKYI
                     SKYGMNYSEFSERHSEISHIDMLEKEDDQMDWEIALHSLHSLQKKVNSLKEDGDIPMN
                     ERDYRKRIEDYILNVR"
     gene            37334..37705
                     /locus_tag="PLF66_00200"
     CDS             37334..37705
                     /locus_tag="PLF66_00200"
                     /inference="COORDINATES: protein motif:HMM:NF039803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6516 family protein"
                     /protein_id="HMZ62479.1"
                     /translation="MFDRGYIESIIQKYNDIVQNYEIITDLFEDNKYTLELRILFNDN
                     SKLDNRDVYLFIDNLIKRKYKFQWMKADNSLIMRWDNAEHHRTLKNFPFHLHRFKKEN
                     VEESPEMTLESVLSFIKNFSG"
     gene            complement(37771..38058)
                     /locus_tag="PLF66_00205"
     CDS             complement(37771..38058)
                     /locus_tag="PLF66_00205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62480.1"
                     /translation="MSKEISQEEIESLFKEFYLLRRKISGLCGKYNISRYQFYKLKKN
                     YSKKSYYDYSLEEWVHACLKTNPNASPEEIRKYLSYVNKTEYTIQEIEIFL"
     gene            38195..39517
                     /locus_tag="PLF66_00210"
     CDS             38195..39517
                     /locus_tag="PLF66_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002999804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LruC domain-containing protein"
                     /protein_id="HMZ62481.1"
                     /translation="MKTTILLLSFILFFGCSSKQDPNYLWLVGLAQSTAAAGVGSPGA
                     STPNVPANPSVPSPVGPQNFTMTVSDNTGTPTDFLFDTTKTLTLDIVAYDFAERPATG
                     ALVQIGDANDPDNFTTYFQAVTNPNGNVTGSFTVNSTTSQVNLIVTYNGIQYKALIDI
                     KGVYGIRRSMYMIGVSQVAIIQDTDKDGVPDSEDIYPNDPTRSAKVNYPPSGYYTVSF
                     EDLYPNQGDADFNDYSVRVRYEEDLNAKGEVSRVRGYFQHVAKGAGYNHILRLSLPSV
                     NASSYTLKRLSNADIQEYQTSGSNVAFQEIEILPASNTTLSQSNTASGQTYTLGKKAE
                     LEVILSNPVPKTTLGAVPYDLYIYVINTKYEIHFAGKFKKADGSDQYLDSNGFPWAVL
                     IPGNWKWPYERVDVRKSYSGFQPWYQSKGTTNLDWYNFPNLSEVFPSN"
     gene            complement(39593..40363)
                     /locus_tag="PLF66_00215"
     CDS             complement(39593..40363)
                     /locus_tag="PLF66_00215"
                     /inference="COORDINATES: protein motif:HMM:NF019224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1554 domain-containing protein"
                     /protein_id="HMZ62482.1"
                     /translation="MQNRIRIILENSDRFKFLKRKSQFLNRLRFILTTIFLGIFFFSC
                     SQDFPKGKLFDVVILWKLIPVTPVPTSNGTPTPVTPTTNDKKYFFITANTNANLGGIT
                     GADSYCQSQKNASFSSLAGIASEYKAFLVDGTTRIACSTANCSGGVSENVNWVLKANT
                     DYYRTDDTKILTTNSAGIVNFGSGSLLAVIDTSGSIFWWTGLATDWTFNSACTVAWAS
                     TTGLASRGVGGVTTNTSIDTGSLQNCSTSTTKLLCVRQ"
     gene            complement(40377..41264)
                     /locus_tag="PLF66_00220"
     CDS             complement(40377..41264)
                     /locus_tag="PLF66_00220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HMZ62483.1"
                     /translation="MKPYLFDFKQFPKPLKFLHLIIKSRFLVLFILSFIVSSLHSEER
                     RIFSYYIEWQNVPGAKGYMIQIKDRAANTEREEKLVQNNIELKVPSGYYEYRIASINK
                     FGKPSSWTKWEEFYVEKDKPKPLKKDEKQVAQEVVEVEPKRNFQKWKWFIPGWTQLEN
                     NQKIKGSLWILWFTGLALYGNMERLAGNRIASDPMNDPIYLSAISIGLKTPLLGDIYL
                     RERRNDAESEYNRHQTNQVAVGVVAILSYALQVWQARKISTSNVTIELNQRSESFGQR
                     VYQAENSMYSFEMKFSMSY"
     gene            41480..43516
                     /locus_tag="PLF66_00225"
     CDS             41480..43516
                     /locus_tag="PLF66_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010416748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HMZ62484.1"
                     /translation="MNSTLDPDQVLEMILEHSLKFSNAESGSLMLVNSRESVLDIVTY
                     RGHDPEIKKEFRLKIGQGITGLVASSGRGMIVNDVSKDPNYISIKKEIQSELAVPLIV
                     EDMVIGVVSLDSNRINAFTEEHLKIVGILANQAALIFKNLQTFRRLEQKNKIQQVLIE
                     ISKIVTSSLDLDEIFQSIMATIERSLKLEKGSLVLYDNASETLRLVAAVGLSTDEIEK
                     GVYQLGEGVVGKVYESGEAMLIRSVANDPLFLNRVGYLNHFKNDPHNVSLLAAPVKSE
                     QSIIGVLSVFLVHQKNMDTQTYLDFLQVVASIISQALKIRQLVDDVKKEISRENILLK
                     RELKEKYKFGSIIGRSKNMEKLFEKIQLVSDSRASVLITGESGTGKEMIASAIHYNSN
                     RSEKPFIKINCAAIPENLLESELFGHTKGSFTGAVSDKKGKFEIADGGTIFLDEIGEM
                     DLNLQSKLLRVLQEKEIEAVGSLKVKKVDIRILAATNANLETLITEKKFRSDLYYRLN
                     VVNIHTPPLRERTEDIPLLTIHFIEKYSKENNKNIKGFTREVQKLLALYPWPGNVREL
                     ENVIERAVVMTQNSMLDESDFVEITEKLQSMNLPIAKVSEETSSDLSAGLYSMPNLDA
                     IDGRALEVVVGEVEARLIQYALKKFRYTKTRVAKFLGINRNTLDKRIKELKIEY"
     gene            43706..44611
                     /locus_tag="PLF66_00230"
     CDS             43706..44611
                     /locus_tag="PLF66_00230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62485.1"
                     /translation="MKKNIFLIMLFTISCGSGEVKKPNLQQETMRELLREDFLLKNYA
                     TIEMNRGKNITAVLKFQIQKMKELEDRHKGNIPAEFRDKYALHKDEYEIALHDIETKE
                     IDMVAKGVKWIISAFGSPTAFVFSTIKTAMELNKNLNDQLKHPRINQTYESLKRYAEA
                     SNVKLVDDEQVSNLLPNAYTIKGEIDSKKEWQTLYGLVTAKSNLIIETTGQWSITNNG
                     NQLNANGTDKSPSDWFDYRVDKKFNYGALICRIVQTEKEKDFPIFSLGNSTADKDGVL
                     ECRINDTDTGNNTGSLEVSIIIKKS"
     gene            44658..45320
                     /locus_tag="PLF66_00235"
     CDS             44658..45320
                     /locus_tag="PLF66_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002975091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HMZ62486.1"
                     /translation="MTLFKKAIVTAGPTREWIDPVRYISNASSGKMGYNIAQEILSWI
                     PNTVYVHGNVSEKYAKVTGAKNLYAETTIQMRDTLLQELTEDTLVIMAAAPADFRPKS
                     IAENKIKKESEGNLKIELEKNPDILVSLNSLIQNKNIKGSVLVGFAAETEKLEEHALG
                     KLERKGLKYIIGNYVGTNSGFGEVESSVRIFGKSGLLKEIQTQSKELIAKEISLFLRD
                     NA"
     gene            45326..46657
                     /locus_tag="PLF66_00240"
     CDS             45326..46657
                     /locus_tag="PLF66_00240"
                     /inference="COORDINATES: protein motif:HMM:NF012730.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HMZ62487.1"
                     /translation="MKVFSMENTFKRYWDLFIFLLATYCSIDIPFWILFPYPQTGFFF
                     FFELFISVAFLLDLIFNFRTSYYKNETLITDPWGIAKNYFFSWFILDLLSVIPLELMI
                     EHGFMPKSLLFLCIFRFLRIIKLTDGVRFKRTWGMHEFDSTSFMRLAFLIYWIGIFAH
                     WTACGWIVIDGGAGEVSNPDTVTRYIRAIYWSVTTLTTIGYGDITPKNNVQTIYTMCI
                     MLVGAGAYGYLVANIASILANTDILRTQFIDKIQKINTFMRYKKIPNEMQKAILDYYD
                     YIWKNRKGYDENQILKELPPSLRMKVSIYLNSDLVRKVPILKNASDELISKIIVNLVP
                     VVYMQGDFIFRKGDTAHHLYFISKGQVEIIGEDGITSIGILSEGSFFGEIALLLDTPR
                     TASIRAIDYCDLYYLDKETFKTIIKQYPDFAAYVDKLIKDRQQSRAELKNE"
     gene            complement(46632..48545)
                     /locus_tag="PLF66_00245"
     CDS             complement(46632..48545)
                     /locus_tag="PLF66_00245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62488.1"
                     /translation="MSRKNLVSFILLCLIQIPFLILSLYFLFSEFNTICPNKDFLSWD
                     ADLRFIKTLKLMDSIRHFEFLSFAFQLLDSPTWPILRNLIQIVVFFASGVSTTSDTVI
                     TLVTFAILLGLAIHVLFYLLDKKWISVLLFLPVWLSVFLSPPILIYTFSAMLEVQGAV
                     FFLASVYYLHLFYRDDEFIRNKSILIKLSLSTFALFQTKYPYGYLFIVAMLVLHVSLY
                     LDETVLFFTRYIAFISHNLKKQYRLLLVILLIVSYILFPESLLKGKTKNYIKYLIILL
                     LSFDFYLYLNHQMSQLLNLKFNRLIGIFQWVFLPIVVFVLIHPDRFSSSSSTLGHIQS
                     EGHAVGEVVEKGLDYYLVFFKTLSSQAFIPEWVGILFLIGLIVGVVIGYIQYYKNKKI
                     ELYFLFSLFPILTILILTFFTPNHQARHIYHLLPTISIAFMLLLSSLQEKYKLVFYIL
                     VLFLSIGLIFPFGQKFASKFSGENICYTGKNKDDFFTPREVEKLFKDVKENAIFFNFI
                     NPDHVNKADTELTLSKLSYLNKLKWSIDPKIFKKHPREEGYSAVYLISDSCEKDYLPE
                     AWFKKLHNSIQLKKIEDPKLSIQYSVESKNHSFMIGPPFPARNFMDRKIYKDVKFSTG
                     EGCLQVIHFSILP"
     gene            48652..50721
                     /locus_tag="PLF66_00250"
     CDS             48652..50721
                     /locus_tag="PLF66_00250"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HMZ62489.1"
                     /translation="MSEIQKVESRSVGMFVIPEITARRLNKILRTFVDHEQGKDIEAI
                     LESIVDPATLSRIMRDNHFWISSANEQKILSKIQQKIPLEEIFFNMGKEFFLADCFEI
                     LPTDDVQLEFEELISRIPILINQYIRYIEIKTISYSKNKLSIEFKFDKKIKENAYDLI
                     FLRGILEGAAILFQVVGCKTTLLETSLEKMDERFFETHSNTKFNSKKCVVQITWIDCK
                     IKVGKTNINKISPAFETKTFVISTLNDSNQGEYSYIDLNDIFSKSKELYIENRDLEAA
                     VEVLKSLRNELMIKQKSITKDLKIARNIQRGIIPQRIPDWRGLQFAVSFMPMQEVSGD
                     YYDYFNFGSNRLGILVSDVSGHGVPAAFITAISKLLFTNYKLDSPSEILGNANSELLD
                     LVKQQGYLTCFYGIFDSNYEMTYSVAGHPRPILLRSKTKEVVVLEGEGTFLGMFDDAR
                     DHFRDYKVKLEPGDKLFVFTDGLIEAVNDAGEQFQQEKLIRFILDSADMDVKSSIDYI
                     IKNFNQYCRGTDQGDDITLLGIGLSLNMEAFDKHHLEAERNFNNKNYELAAEHLIKAN
                     SILPNDLSILLLLGKCYAKTRNYVEAIKYLEEYNSLKTDNADSHLILGYCFYKLENYG
                     RAELELQKSISLRSGNLPALYNLAKTFAKENYPDKALDTINKILVIDPYYTPALKLKN
                     FLMKKLS"
     gene            complement(50730..51584)
                     /locus_tag="PLF66_00255"
     CDS             complement(50730..51584)
                     /locus_tag="PLF66_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000142697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase"
                     /protein_id="HMZ62490.1"
                     /translation="MAFTNKQSNFQSSIDKSKIFYQSWTKPKANRVLILQHGFGEHSD
                     RYGNIIEKLKDDNFNIYALDARGHGRSDGKRGHVAQFQYYIEDLTELIYIARQEQKLN
                     KVTLLGHSLGGVIALQYSLEGFNQDNLDGMILSSPGLRPKMDFEKEVKKVMAELLASF
                     MPDMTIDANLDLNYLSHDKSVIEAYKNDRLTHGKISFQMATNLFHLSKALYEKASHLH
                     IPVLIVHGEQDGIADVNGSRDLFRYLTTEKKIIKTYPGLYHELMNELPKDREMVLNEI
                     KDFLHSIG"
     gene            52211..53329
                     /locus_tag="PLF66_00260"
     CDS             52211..53329
                     /locus_tag="PLF66_00260"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HMZ62491.1"
                     /translation="MSKLTSVFISILFILLLDSCSSLFQKSWKPTDKELLKTIRVNLG
                     KFDSSQEIKSKGKIHLQTQSDSRDIDYIFSFQPSDGKEKYKLTFDTNQFSLRDIPYRG
                     ELEIIPEVNSGKFIFVNKVPMEDYLMGVVPAEMPSSWPLEALKAQAVAARTYAVDSIL
                     AAPNSTYHVESTTQSQVYGGIKRETVRSNQAVRETVGEILIFQNKPARTFYHSNSGGK
                     TESPEFVWGYTGLNYLVAIDSPHCKLADNFQWKITLTRNQMQEKLKSLELEEIKNIRI
                     SEYTPSGRAKTLEVEGKEKVVTMNAVDFRRAIGQTVMRSLLFDIDLEEDTFKIKGQGF
                     GHGVGMSQWGSKGMAENNYDYKEILSFYYKGTDMVTID"
     gene            53329..54522
                     /locus_tag="PLF66_00265"
     CDS             53329..54522
                     /locus_tag="PLF66_00265"
                     /inference="COORDINATES: protein motif:HMM:NF015418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HMZ62492.1"
                     /translation="MISVQKAIELILDNTHTTNRTQKLPIYYAKNFILAQEIFSDRDY
                     PPFNRATMDGYAIRFNDFSIDRQWNVIGEIYAGDNWDYPNLNRELPAIKIMTGAAVPE
                     DFDVVIKIEDTNRVGNKIHILSEKVKQWMNIAKKGEDTQSGNTIVSQGTLIDDSILTI
                     AGSMGQLELNVFEPPTVSIFSTGNEIVPMGQIPNSNQIRDSNTILIESMFQNFKSKIK
                     RKVLLPDDLEIIKNHLDQSSDSDFCVITGGVSAGDKDYIPKALESIGAKEIFHKAMIR
                     PGKPIWFGKKDNTLFFGLPGNPFSARVTFKVFIEPALYKFMHLNKPKSIFLPLLSERK
                     KKHGMEEYFRARIVNKENKSYIKEISSNGSGDYLNSFGTDGIAIHPANNMELPQGINL
                     EFLYW"
     gene            complement(54523..55725)
                     /locus_tag="PLF66_00270"
     CDS             complement(54523..55725)
                     /locus_tag="PLF66_00270"
                     /inference="COORDINATES: protein motif:HMM:NF040144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5683 domain-containing protein"
                     /protein_id="HMZ62493.1"
                     /translation="MRIKYLLLFILIAANSIIAQSTENNPKKTSIKWKNVENAIGYKL
                     EIRPDKEKEMQINSTENEVFLDLKPGKYEYRVGALNKFKKVKHWSKWSSLEVIYSQPP
                     IVEENLKYTFTIPEKKQDIVVKGKYFLEDIEVELESSNGIVPINEIKRVSESEIKFSV
                     NLETFTSPDSFRLTLRNPLNKSTSVDSFVLLQEKPKSAEPNIAETKDTKPDDPTKIDK
                     DNSVAKETREGTDTTTEVTKGEPKKSYFAEVFPPLWRSSLIPGWGQYYKGEKTKAYIF
                     GGSVVFFLSSVFFFESYKQYSLSRSDQFSNLLFLLPGDRNLLPAAIYLRSQSIESRNN
                     AQEEVNKQEAVVGVATAIYLYNVVDILINRKSSDKIINAPEKTSGLFFNMGNQGRGSF
                     QFGFSYRF"
     gene            complement(55741..57882)
                     /locus_tag="PLF66_00275"
     CDS             complement(55741..57882)
                     /locus_tag="PLF66_00275"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HMZ62494.1"
                     /translation="MRSLNSNRIIVSVLLFLIFFFSVLLYIDFKRKIGVGAEEVIGII
                     RYKEKLIQRKIDGQVVWETVDRDTPISNRDSVRTADNSTVSIELNDKTQIDMDENSMI
                     LLDFSNQNINLDFAYGAFAAKRSENQADTKLNIKSGSKTIELNKSDVKLTKVDEKKLD
                     IIVDKGNATIKTKEGESVIQKDQLASLKEEKVEIHSVPVKLNKPADRAVILGNEISAI
                     NFSWDVQTGSNADFETTLEIAEDYKFSKKLKKIPVKKEKNLELKLPVSTYYWRIITNS
                     KNLTPEVSESRKLSIIKEEPIRLFSPVNNTKYPLISTNPIINFSWNTSEYASSYKLEI
                     AKDIEFKEIIKTIDSHTNRFAIDSLGEGRYYSRVSARFLSQEVPPKVSESVTFSIEKK
                     VDLDPVEIAAIENTATTVTAIEKEKFVLNWKGAPEYSSYEVQIAEDKAFQNIVETQKV
                     NRNFLVPKSLKKNTYFWRVKGLTKDGKSSNYSSSRFEVREPASLELLSPADNAEFAIK
                     PNEKIAFKWNKIDIYGTYKLEISDKPDFKTIYKSEMTTSFGVSLDGFTSGTYYVRLTL
                     INQETNEELLRSKQKSFRIPLSISEPVLLYPVKAETVDMSDSDVLQFKWDTVPNAISY
                     KFEMFQKVENGDKLLISDDTIANQYTIKDLNILDEGKFKWQVKAKVKSSEGKEYISPI
                     AMDYFSISLKTSPSTAPEIISPRKQHIDKKF"
     gene            complement(57866..59758)
                     /locus_tag="PLF66_00280"
     CDS             complement(57866..59758)
                     /locus_tag="PLF66_00280"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HMZ62495.1"
                     /translation="MEANKTDFKLSKFTIRVKMISIISLIMVVSISLMILIASYAFRK
                     DSEIRVEELNIKLAEVIGLKVKTDIESVIEKTRLMANVAEQRFNSEKDKEKLVEAFFK
                     DDINFMLFGIFSEDKGVLKAKQVNFNNKYLSENGVSEEDLQKLLNIHSKELIKAFTGE
                     SVIHNASQGFPYPIVAIALPHRSDYDSRTILITLFRMEKFLNAFKTAGISENFMVNGE
                     GIVLAHPNSNIVLSGTNLSESPIVKTMMKSTVDNGLTRYEHEETKKYYLGSYKKIGFA
                     GVGVISTVLEEKAFQEVYNIQRRNLLILGITLCTAILIVLFFANTLTSPILNLVDATK
                     KIEEGVFRVDIKPTVRDEVGLLTNSFVKMGKGLEEREKVKDALGRFVNKEVAEMVLKG
                     ELKLGGEKKNCAIFFSDIRSFTAISEKLDPQEVVEFLNAYMTEMVRCVNETHGTVDKF
                     IGDAVMATWGAAFSRGNDAENAVNGALMMRSVLLKFNEGRGGDKKPIIKIGCGLNYGP
                     VVAGQIGSSDKMEYTVIGDAVNLASRVETLNKPFGTDILISEDLYLLVKDIFKVEKMK
                     AIKVKGKEAPQTIYAVIGRFDDPNCPADLAAVRKLVGIKYEEPKPSSGKEDEDEEKEV
                     KYEIIE"
     gene            59924..60439
                     /locus_tag="PLF66_00285"
     CDS             59924..60439
                     /locus_tag="PLF66_00285"
                     /inference="COORDINATES: protein motif:HMM:NF019819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HMZ62496.1"
                     /translation="MRFIFFFLFLLIQCGSSQEFRQTVPKVDLNRFMGKWYVIASRPT
                     SFEEGAYNAIEIYTYNQERDQIDIQFTFNKDSLTGPVKSIPQTGYIIDRENNSYWKVS
                     PFWPLKFDYLIIDLESSYQWSVIGVPDQKYIWIMARTPTLPETTYNQILERVKGLGYN
                     LSNLKKIENKP"
     gene            complement(60436..60978)
                     /locus_tag="PLF66_00290"
     CDS             complement(60436..60978)
                     /locus_tag="PLF66_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020783682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HMZ62497.1"
                     /translation="MTNVPKEIVIAVTGSIAAYKTCELVRNLTKDGYPVRVIMTEHAT
                     EFIGRITFEALSGKPVRINEYDTGMAHIELKNIASVFAIVPATANIIGKFANGIADDL
                     VTSTYLASTCPILLAPAMNPGMYMSPAVQRNLKILEKDGVQILDPSSGMVVCGDEGQG
                     KLADLSLIQSKIIELHKKNS"
     gene            complement(60971..62308)
                     /locus_tag="PLF66_00295"
     CDS             complement(60971..62308)
                     /locus_tag="PLF66_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010577037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HMZ62498.1"
                     /translation="MALIETFIILFLVAVNGFFVAAEFALVSLRPSRLEELIKENRPM
                     ASITKSVLNKLNDMLSVCQVGITIASLLLGWLGEKFVAVGLHGLLNLVDFPGKESWNL
                     EGVSITISFIFITFMHITLGELIPKSIAIQSTEVIALNSSLPLLFFYYLFYPITYFMN
                     GITNQFMKTLKLNNLSHKFVHSAEELMILLEEHTKQGNIDNAELQIIQNTFEFSEHEA
                     KEVMTHRLSIVGFSAEEKIKEILPVIAKNHFSRYPVYNKTTDKIIGVIHVQAVIEWLA
                     RTNRNKNATVKSIMQAPVFVPETLSIEKVLQKLRKYKQHMAIVVDEYGGVAGLLTVDD
                     IMEEVFGPIHDETDVKVEAIEIKSDSYTIDGEAELDELEDIFNENEIEGMKDVRTIAG
                     FFLEKHQDMPSEGSSITITKGTLTVEKMQGNKILNIRFDMAPLNFVHTKEDYD"
     gene            complement(62411..63307)
                     /locus_tag="PLF66_00300"
     CDS             complement(62411..63307)
                     /locus_tag="PLF66_00300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62499.1"
                     /translation="MFLIRIATLKCLSILFFLFFVALDIYSQSSKEIPFLRATETALQ
                     TSVYTLVSKDTNVRLKIIGVVHVADKEYYLNIQKIINELDFLYYEGIRVSSKMHSKNF
                     SKNYYAQTLEEMDTRRSIKKYSDLKSEIARFLNFADQSELLKPKQNWINADMDFQQFV
                     EMLKNYKIGFDSLSTNFSNDSKSISKDYSELMQMENTDSAEYSTKVLSFKKKMSQNLV
                     KSAFDLCFLEEMRLPREAIIIERNKIALSFVKPKFNSQVPLELGLLYGAAHTPNFVEI
                     LTREYNFEIESYEWLDAWSLQN"
     gene            63436..64518
                     /locus_tag="PLF66_00305"
     CDS             63436..64518
                     /locus_tag="PLF66_00305"
                     /inference="COORDINATES: protein motif:HMM:NF019403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase
                     domain-containing protein"
                     /protein_id="HMZ62500.1"
                     /translation="MNETNFTSIQRNSTLDVLRGIAIVSMILSNFAGELLKAPHPILV
                     TWIGSLAAPLFVMIAGYLVGFSVTNKNYPFSYFLLRGFLTLLIGVCIDVFLWRSYPFI
                     SVDILYLTGLSIPITSLFMRMNLSFKVVFIFLFFIFGPLLRMQFGYSDKPSFPPIWKD
                     GNFVPFAGDFFTIVKHWFIDGWFPIFSWMGVSFLGGFLAELKIKHGDLISKDKIYLLL
                     LSFFLLLVGAFFWNHEFRLATRGNYTELFYPPTLSYYLVYIGLIFILYLIVDINSNSF
                     LYSPFRLIGISPLFFYLLHFLIIAIVIAPFFGKTPEKEVYLDLNVFLISFLFIILVCI
                     LFSFFISLFKKKFSNLPFLVKFFIGG"
     gene            complement(64851..65393)
                     /locus_tag="PLF66_00310"
     CDS             complement(64851..65393)
                     /locus_tag="PLF66_00310"
                     /inference="COORDINATES: protein motif:HMM:NF009807.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal-dependent hydrolase"
                     /protein_id="HMZ62501.1"
                     /translation="MNKQNEANQNYPVGEYSHPDIISNDHITGWIEEIEHFPKLLRET
                     IQGWTEEQLSTRTKPDGWMVKQVIHHLADSHMNSFIRFKLALTEDSPKIKPYAEDRWA
                     ELIDGKDSNIESSLQILEGLHKRWVVLLRSLTLDDLEKSFFHSEHNKFFKLAPTIGLY
                     AWHGRHHLCFIQKLKQRMKW"
     gene            65503..65946
                     /locus_tag="PLF66_00315"
     CDS             65503..65946
                     /locus_tag="PLF66_00315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62502.1"
                     /translation="MTQIIKILIISLLCISFCNLNALHNKKQEKKTKSAPKKKLYDCK
                     NYHQGKFTYTDINGLKILIIRENEIQYLYREYPNAYKKKSIVWLTDCKYKSTVIEIHD
                     PILDDSMLGKENIVEITKNRNQDWYEYSNSENDVLYTGIVYVVKD"
     gene            complement(65936..66454)
                     /locus_tag="PLF66_00320"
     CDS             complement(65936..66454)
                     /locus_tag="PLF66_00320"
                     /inference="COORDINATES: protein motif:HMM:NF027788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HMZ62503.1"
                     /translation="MSYIMVDVEADGPIPGEYSMISFGAVLVDSTLDKTFYGKIKPIS
                     NQFIPDALAVSNVTREETLSYSEPTIVMKEFSDWIKKNTKGQPMFISDNNGFDWMFIA
                     YYFHRFYGSNPFGHSSTNLGSLYKGIVKNTFQNFKHLRKTKHTHNPVDDAKGNAEALL
                     TIVKEYGLKISL"
     gene            complement(66461..67624)
                     /locus_tag="PLF66_00325"
     CDS             complement(66461..67624)
                     /locus_tag="PLF66_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010577297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HMZ62504.1"
                     /translation="MDFRMTDEQKALRDLARDFAKNEMAPKAEHHDLTGEYPMDILKK
                     AWETGLMNLHIPTKYNGAGMGDLDDVIMGEELYSGCSGMATAILANNLALAPVLIGAS
                     DEVMKKFVEPMTNEFKLAAYAVTEPGAGSDVANIRTTATRVGDEYVINGSKMWITNAG
                     VADWFFVLTKTDPAAGHKGMTGFIVEAKTPGVIIGKKEKNMGQRCSDTRGVTFEDVKV
                     HKSQMIGNEGDGFKIAMGAFDHTRPGVAIGAVGVARTAMEHAIRYANTRNAFGKPISS
                     NQGISFMLADMAKDIEAGRLLCWQAAWMIDNGHKNTYQASIAKAFCADMCVRICTDAV
                     QVFGGYGYNTEYPVEKLMRDSKIFQIYEGTSQIQRVIISKFLNDGKGIEHPNN"
     gene            complement(67693..68241)
                     /locus_tag="PLF66_00330"
     CDS             complement(67693..68241)
                     /locus_tag="PLF66_00330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62505.1"
                     /translation="MKLKLSILLCLFLFNACSNPIDKKRRELENCKITVDSLEIKNVR
                     MFIVPPVPKLYFKANIQIENTNDVPVTIEKFSFKISTVESGNENAYLAEVTNTSEKEI
                     PALSTEIIQADMQTLFEENVNKKLVIFLADLIKAVLAGGEMEFILEGSVEFNTMLGKM
                     NIPIKEKVKTSLRKTKKPEKKP"
     gene            68412..68927
                     /locus_tag="PLF66_00335"
     CDS             68412..68927
                     /locus_tag="PLF66_00335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62506.1"
                     /translation="MIKKKIRFFSSKILLTSILLFSLNQCLPKVEFWIINDSSYDYKI
                     EILFKRKYTLEGFGALFGYKLEKMPAIAKFPSPSYDEMFFQGDRFKKDAERITEADFQ
                     YDPNTAVFSYTLKSKMSFLLERSSELEHSRGIDLIQELRFISKDGEIIYKGKEIRKAF
                     LLSKEITILLK"
     gene            complement(68950..70959)
                     /locus_tag="PLF66_00340"
     CDS             complement(68950..70959)
                     /locus_tag="PLF66_00340"
                     /inference="COORDINATES: protein motif:HMM:NF021778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HMZ62507.1"
                     /translation="MFNRIAFFVLTIFVLDCNTHVNKSLGGIGFLSYIYRYFSSKSCI
                     TYAKTVNSTNSSGSNNSYTCNFDISRLSYDCIYTNPITVDNISTTVTTEFYNSTFDFI
                     NRVQNFTIYTMRYSQINNDSSTNKFFNYDNSMRLLNIIQSDGTTVNGVEYDSKNRVIK
                     GNIMLSNSVCSVPFVNTYDDTNLIFSQTLQFSQTYPNTNLFCYFISTTLSDQKTEAYL
                     DKNLFLSKRIFTYGANVTTETYTTVDTARVCTGDDGVPYSAPIIQKINLPGAAVGETV
                     TITGANFDLDPSANTIQFVNGIATNGIASNGSSLSFVVPSGAASGQIKVVNRFGMANS
                     KNIFYIYRYFLYTANNGSNSISSMELDSANGSFSNLVSFAAGTNPQSLVAAFNGKSLY
                     AVNSGSTVLNNISINQTNGTLTGVNTATVGNGLKSIAIDSTGNLMFVVNSTDMTISRV
                     LIDQNTGNVVVLGATNVGVAGLYSIVTHPSLPFVYVTANTSSKVYGYTIDFNTGNLNP
                     MIGSPYSVSANPLGISIEPNGSFLYTANAIAGSGISRFTINQLNGSLSSELVVNANLM
                     ETITIDPSGKYLFACSLTFNTIYSYGLNSNSSPSLLNSIPSNQCRNLLIDPQGKYLFA
                     TEDVAGLSGKINVFTIQPNGNLAVNSSLSLGVNNPRGIALAKAQR"
     gene            complement(71115..71888)
                     /locus_tag="PLF66_00345"
     CDS             complement(71115..71888)
                     /locus_tag="PLF66_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012387745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HMZ62508.1"
                     /translation="MNVFFFFSKILTVILFPLPLCILLGLLIVLFKIKGGRNKFLSFL
                     PIGLLWFAASFPVCQSLITSLEKEFPPIKVKDAPRADAIVVLGGMINTLSPFPDRVEI
                     LGSGERLTDAILLHKAGKAEYVLFTGGSGLLFDQEVKEAGFAKKFLIDFGIPENKILL
                     ESESRNTFENALYTKKILEEKKIERIILVTSAFHMKRSIAIFQKLGIEAYPFPTDYRS
                     LRMDLNWEIIIPATGALETTTIAIKEWMGILMYQWKGYM"
     gene            complement(71885..72748)
                     /locus_tag="PLF66_00350"
     CDS             complement(71885..72748)
                     /locus_tag="PLF66_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010570676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HMZ62509.1"
                     /translation="MKSSKSEPNIIFKRKFFTFENLKLSYIDTETNSKDIILITHANG
                     YSAECYSYLIEAFANEFRVIALDFAGHGESDPYYDFSNWDFLRDQILALIELENLDRI
                     ISIGHSMGGASALRASKKKTSHFKKMFLLDPTFLSIPIIVYGKIFGSQISQNAAKRRR
                     HFKNLEVVRRSFRKFSAFSNWDNRVFEDYLRSCFRKKGEEIELICDPTIEAKYFKTPS
                     LKSFKDYGKNKLETHIIIPQKFEVCSPYRAKIIISGNKHSTLTILPDGTHFFPFEKPD
                     FTIQKIKDHIR"
     gene            complement(72764..73159)
                     /locus_tag="PLF66_00355"
     CDS             complement(72764..73159)
                     /locus_tag="PLF66_00355"
                     /inference="COORDINATES: protein motif:HMM:NF017020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HMZ62510.1"
                     /translation="MKTDVVKNFLIKAEHDLGSAELIYEKKPEYTDTITFHCQQTIEK
                     SLKAYLTYHNVKIKSNHDLIYLIGKCSELEQEFKSFSQDIYADVNDIGMSVRYDDIKN
                     DPKVEEVFRYLEFTREVFEFGKQKIKMQS"
     gene            complement(73156..73497)
                     /locus_tag="PLF66_00360"
     CDS             complement(73156..73497)
                     /locus_tag="PLF66_00360"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HMZ62511.1"
                     /translation="MINQAITLNDETVIKFKNLVYKLEAPVNRIYLFGSRAKGNQSLE
                     SDYDFLVLLNNINDGELMKKYRRTIVLNAHKEIPDVPFDILVKNLEEFEAYKMQMNTL
                     YNEIFKDGIEI"
     gene            complement(73514..74833)
                     /gene="add"
                     /locus_tag="PLF66_00365"
     CDS             complement(73514..74833)
                     /gene="add"
                     /locus_tag="PLF66_00365"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004786665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HMZ62512.1"
                     /translation="MKAKFEQILNRISIIDRDVNELNRIKSKLPDDRPYSSGLQLIFD
                     KQINNLLDERDVLLEQTVPNPPAWLVGDVTNGHHKHEVIQTIPTIDPNYKNTEPTEQE
                     VMAFIKALPKTEIHLHLEACVNKQTLKEMFKKNGVEITEEEFEKKFMFHDLNGFIQLF
                     LFIQSAVKSHEDFSFMIDSLASYMRADNIVYTEVFVAPTKFIQNGIDFDKMIEVMVTR
                     IRELKAKEGLEIRLLIDVSRTFGAENAMNNLNRVLNLKYPEVLGIGLGGAELLGPAKD
                     YEEVFKKAREAGLHAVIHAGEDDGPWSIWDSINYLKVERIGHGTSAILDPDLVEYLKE
                     SQIPIEICLTSNVFTGKYVKKEENHPVREYYDKGVYTCINTDDPEIFDVNLSYEYFKL
                     YRFLNFTIEELIDLVKKGVYATFHPDKDALWKKINSEIIKVRTKHNF"
     gene            75015..76208
                     /locus_tag="PLF66_00370"
     CDS             75015..76208
                     /locus_tag="PLF66_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004785484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HMZ62513.1"
                     /translation="MRTMRKTFLPFALPSISEEAIEEVAAVLRSGWVTSGPKVKQFES
                     EFRDFVGTKDAIALNSATAGLHLALEAIGITEKDAVITSSVTFTASAEVICYFGASPL
                     LTDVDPIHNIMTPDTHRDLIERECQWNGTHLIHKQSQKTIKAVIPVHLAGYTCDMERI
                     IEIAKSYNLFVIEDAAHAFPAKHKSKMIGAWGDFTVFSFYATKGITTGEGGMVTTNHS
                     KESDRIRLMRLHGINRDAYNRPGWYYEVVDAGFKYNMTDIAAALGIVQLHESVGFWNR
                     REEIALKYNEAFSDLEGMKLPLPDERGRHSWHLYRIELDPYKAKLGRDSFVEELKERN
                     IGTSLHFIPIFEHPYYKKTYQFNRKNFPNACAMYDKAVSLPLFAGMTNTDVEDVIHEV
                     RELLK"
     assembly_gap    76535..76634
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            76784..79645
                     /locus_tag="PLF66_00375"
     CDS             76784..79645
                     /locus_tag="PLF66_00375"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HMZ62514.1"
                     /translation="MYSKEEALAKITVLVKRFEAQIDTYRKSDYNETLTRRDFIDPLF
                     EALGWDIDNKQGASESYREVIHEDKIKVGGATKAPDYSFRLTGGKRLFFVEAKKPSVA
                     VKDDMLPAYQVRRYGWSAKLAISIITDFEEFAVYDCTKKPNTTDKASVARIKYLTFKE
                     YISDFDFLWNTFSKEKVIQGSFDAFIKNDTNKKGTTTVDREFLNSLDNWRTILATNVS
                     KTNLELNEDEINFAVQQFIDRIIFLRIAEDRAVEEYGSLKEVISKPIAKAGDNEYYTN
                     LVKLYMKADDKYNSGIFDFKKDKISTSLKIDNKVVKTILTELYYPECPYEFSVLSVEI
                     LGSAYEQFLGKQIKIDKSHKAKIEEKPEVRKAGGVYYTPEYIVEYIVKQTVGKLTESK
                     SPKEIEKIKIVDPACGSGSFLIGAYQYLLDYHKNYYAKNKKSWSLKDSPITPDGNLTT
                     AEKKRILLNNIYGVDIDVNAVEVTKLSLLLKCMEGETEASINNQLRLFNERVLPTLDD
                     NIKSGNSLIDTDFYDNELDFGEDKKIKPFHWQSAFSQIFKQGGFDAVIGNPPYGAYFT
                     SNETDYFLQKYRLQDYQLDSYFLFIEKSLETLQDNGLLGFIIPNTWLLNLNTSKIRIH
                     LFSKTEITNIVHFLNPVFAQAVVDTEIMVFRKTKPRKTHEIKIEIYDKKNEKVENKIK
                     QQNWIDSNGAPVNIFDNPNTQKIKSKIANLPILDAVCKITQGTKPFQVGKGIPPQTRT
                     IVDEKPFVSEKKINHLFRPLLRGSLMNRYQILWDNNYFIKFGDWLAEPRYSAEYDAKE
                     KIIIRQTGDHLNATLDTNQFIVRDNLYTIVNKISKINLKFILGLINSKFLNWYYQNIV
                     NPEKGEALAQVKRGHLASLPIKTIDPKNKTEIQLHDDIVKNVDLLLKLNEELPTIKLQ
                     TKIEEINSRIERAEENINQAVYKLYDLTPEEIQIVEGKD"
     gene            79721..>79913
                     /locus_tag="PLF66_00380"
     CDS             79721..>79913
                     /locus_tag="PLF66_00380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62515.1"
                     /translation="MIRVINMIRTQERPNRIITAIIKIKKITVQTVGMIRTQKPLNTT
                     ITAIIKIKKITVQTVGMIRT"
     assembly_gap    79914..80013
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            80141..80542
                     /locus_tag="PLF66_00385"
     CDS             80141..80542
                     /locus_tag="PLF66_00385"
                     /inference="COORDINATES: protein motif:HMM:TIGR04256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HMZ62516.1"
                     /translation="MINEAYKYSDLTSKIIGCAITVHKTLGNGFQEVIYQRALEIEMG
                     VAEIKFQREFEMPIHYRSKRIGTRRVDFLVEEVISVELKAITKLEDVHFAQAINYLEA
                     YNLEIGLLINFGETSLNFKRLTNKKFSKENI"
     gene            80601..80852
                     /locus_tag="PLF66_00390"
     CDS             80601..80852
                     /locus_tag="PLF66_00390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62517.1"
                     /translation="MITDTELKMKAYGALREKLNLVDVERFISIIQKEKFDYTKWREN
                     ILEDLSLDELSDKAMNYYKKQNSPSKKKIKKINRMKKER"
     gene            80849..81100
                     /locus_tag="PLF66_00395"
     CDS             80849..81100
                     /locus_tag="PLF66_00395"
                     /inference="COORDINATES: protein motif:HMM:TIGR03831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiT-type zinc finger protein"
                     /protein_id="HMZ62518.1"
                     /translation="MKKKLAKCIHCGIGKTSIKSVEKILSSNNNTAIVKVNAEVCDHC
                     GEKFYEPETVRYLERIKKELKNKNSKQLIPIGIAYKVAM"
     gene            81142..81345
                     /locus_tag="PLF66_00400"
     CDS             81142..81345
                     /locus_tag="PLF66_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015679773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62519.1"
                     /translation="MRTDTEIRLEGMKILFSNMDIVEAEKFISIIQRDKFDYTKWRQK
                     LYEGLSVRELSKKAMHLRENKEK"
     gene            complement(81829..82113)
                     /locus_tag="PLF66_00405"
     CDS             complement(81829..82113)
                     /locus_tag="PLF66_00405"
                     /inference="COORDINATES: protein motif:HMM:NF014043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HMZ62520.1"
                     /translation="MQEYTQGLSFEDFQNSNMVKDAVIRNFEIIGEAANSLPDSITSK
                     YPEVPWNDMYGLRNRVSHEYFGVDYGIIWRIAKDYLAENLDALKRIKENL"
     gene            complement(82274..82588)
                     /locus_tag="PLF66_00410"
     CDS             complement(82274..82588)
                     /locus_tag="PLF66_00410"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HMZ62521.1"
                     /translation="MPENFLDLSFVKEKLLSLKPELIEKYKIKRIGLFGSFANGTFHD
                     KSDIDILVEFTETPGWEFFRMNRFLENYFGRKVDLVTPEAIKPQVKERILKQVIYIER
                     QN"
     gene            82932..84335
                     /locus_tag="PLF66_00415"
     CDS             82932..84335
                     /locus_tag="PLF66_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015681666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62522.1"
                     /translation="MNKEKLFGIKPNFLTVAYSNFIKSAIFLRYSINPEQKILLGSKP
                     SPILKDLEIFQKGNGVKVENGILVGTVRMGYGHHRMAYALYSWVLAKKVKPYLHDLLG
                     IESNEAVAIKEIDGLYSQMSRLSSEWGGIVEWAWGQITAQGNIGSLYVSTILAESYTN
                     MMSDLPRSMPYISTYPLNGQIAVEAGFKNVIHLIPDNFPQYYLLVPGAMNLVQSPASY
                     HKFIDMGVPKENLQIAGHWVSEPIVTNVEADSKARLERIEKQKPKRLLLAIGGAGAQK
                     NYTINFIEKIKNKLKNGELHLFINAGDHGTLFDTLKEEFEKMGLHYNIVSNWNDLLEF
                     CKTNTIDSTTESKLAPITIFHYTTYFEAFTTTDYLIRVSDILVTKPSELAFYPIPKLF
                     IRRVGDHEAASAFRSMELGEGTTECREPEHAFEMVKLLTEQSSILQRMNECVIRNAKD
                     GIYDGAKNAVEMAMKSL"
     gene            complement(84327..85619)
                     /locus_tag="PLF66_00420"
     CDS             complement(84327..85619)
                     /locus_tag="PLF66_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HMZ62523.1"
                     /translation="MKYSLEQEKILEDESNFKQVIAAAGSGKTSTMIALLEKIILEKK
                     EEPMRILVITFSRKAVGEIKERLEKRVGHQEIRIQTFHAYCLYILQKYHPQFKDVNIQ
                     IIEPNEKESILKEYFKRERFTVGGIPYDFLLSNTGNFLEKNFPSLAIEVNTAYALYKK
                     EKNKLDFDDLVEIYLNGLRKKEDWAILARQEVSRVIVDEFQDTDLTQLEWLRLLSPDK
                     LCVVGDDWQAIYGFRGASTEPFLKFKDFFNPCSIHFLTTNYRSLPEVIETSAIPIGKN
                     KKNIKKKVRPFREGKAKVYKIYIDEEQSLESLCRNIREKAAIDSDLMVLCRSNYRIQA
                     LINLGVPEKNIMTIHASKGLEFKTVFVDLHSGWNLKIEEEPDIIEEERRILYVALSRA
                     KDNLYIFGNRKFRKKLIEDNFFSYFRFTVSECKLANLK"
     gene            complement(86032..87216)
                     /locus_tag="PLF66_00425"
     CDS             complement(86032..87216)
                     /locus_tag="PLF66_00425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62524.1"
                     /translation="MRRSQIKLWIALIVFLGISFNVSERKSNQKDSLLSFGKINLELT
                     GVQQAFAGTAASWGFVRQSATWGRANSTLVDTLLTSLQTSGVFSRISSGELIVPNQTS
                     STGVTFTYRLKINENVSGIASTAYSGTKTYAHRFEAWKSGDSVPTLQMFFSSLTGTGT
                     TGVLLYYNLSKLNPTAFGTATSAIVESYTSGNKGDMKQTYSWNNGPQDSSWISRNGRV
                     IIREMNNGADICVRTVVTMNTNSSGLNGQLNSLCGSNTNIYYLLAYVQKTTAPFYTVA
                     KSGLTNTTPIKNVSTFCGLAGSVSSALPNNYGLFDQNGFVSDGATSSTIPSGYPGTTD
                     VDNAFSRTGAALNELGIDNVAVAEYDQSDATFMNNLSTNASLAFKTSSAPSCSQSFCI
                     NP"
     gene            complement(87213..87407)
                     /locus_tag="PLF66_00430"
     CDS             complement(87213..87407)
                     /locus_tag="PLF66_00430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62525.1"
                     /translation="MNLKKTLIVLVLILSGLYACQKEKEDHSIRNLLLLGTIRNQSTT
                     SSSSSCTSGMIVCIPKGIAE"
     gene            87616..88155
                     /gene="xrtK"
                     /locus_tag="PLF66_00435"
     CDS             87616..88155
                     /gene="xrtK"
                     /locus_tag="PLF66_00435"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR04287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase K"
                     /protein_id="HMZ62526.1"
                     /translation="MKSKKEIAFHTFYLLVIGISFIILKLFYKIADNSDLLFLLKPIS
                     FIIDVFTNSDSFFSLESGYYYPDLQITIDKSCSGFQFWLLTSTFFGFQFFWKRTSNSF
                     LILPMLILATYFLTIIANISRILITIQTRIMLKSFSIPFTSNLHEGLGAFIFLFFFIS
                     ANLSLLYFMRQMNATPSKS"
     gene            88133..90964
                     /locus_tag="PLF66_00440"
     CDS             88133..90964
                     /locus_tag="PLF66_00440"
                     /inference="COORDINATES: protein motif:HMM:TIGR04286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSEP-CTERM sorting domain-containing protein"
                     /protein_id="HMZ62527.1"
                     /translation="MRHLLNPKSIFFITTLPASILSLLMYGNFTLIQTLLEKNSIDLW
                     IQFGIGLSILTILSTLSAILFLWRKLEANISYALYSLIGYTTLLYSYFYKFDSLIPIS
                     IPQWMIADEVFFYAGTFLMPTLLHSIFILVGKLTDNEKQGHVIKNFIYALLIPAIWYL
                     FFQIILPLWKTENHARRDHVLFIFFIVGTILFLFFVTRWIYVLSLSKSHFIRRYELIW
                     KIPFSVLFPLLGLAINNGFNPKSFEFNNYASGIFGDFNGFWFYVVAILNGLLICLPNL
                     ENKFYRYFLFFGRSFTFSYTFYFFLVFIPFLPFSIVGVIWFGLGFLLLTPLILMIIHS
                     NELISDFEFLKNYFSKGQIIFTVCFGILLLPTFIHANFYLDKLNLKSALSYLYSPNYL
                     KEYNLNIKSIYQTIEKIKIHKERNRGFFSFANNKTPYISSYFRWIVLDNLTLSDSKIQ
                     FMEKVLLGVNDTIDGSSIVERIEPNLISNNVIISKVKTKSEYNPDKQFWISKIDLEIK
                     SNSTFFEDEYSTSFSLPIGSWISDYYLFVDGKKEKGILAEKKAATWIYSQIRQVRKDP
                     GLLFYENSGRIQFRVFPFSRGQTRKTGFEIIHKIPIEFKLDGNTISLGKEKDINIYSD
                     DKIIFIPNAIKQKLPTKRRNPYYHFLIDTSISNESDFEVLAQRIETFLSGKQELFSPK
                     FSSVNLYTTSLDPKDWKSQLKKIKRNGGFFLERALRKSLFENYIANKNEFPIFIVVKN
                     TNTKTIFENNLSNLQFTFPETDTYYELLEDGKLYSNSFLNIAERGMHNRNKLNIDFTV
                     YEYSSDGITYSLPQRDSSEIILKKPSFPLSDLDIKEKTWSSGLWLEAKWMSHQLFPED
                     SEREWIELVKYSFISQIMTHSTSYIVLENEAQKEALRRKQAAILSANKSLDAGEETLR
                     MSEPDFYLLFLLFLGYLFLKYFFKRRQFE"
     gene            90961..91278
                     /locus_tag="PLF66_00445"
     CDS             90961..91278
                     /locus_tag="PLF66_00445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62528.1"
                     /translation="MNPSDLLLIGFLFLVGVIFGFISSVFTGVFNHFLINRFLTESIL
                     RNGINVFLISISSFLGSMFGFIGVIGFAADENAGEIFPYYFGISILVSILCSIGYYLI
                     RIR"
     gene            complement(91275..93188)
                     /locus_tag="PLF66_00450"
     CDS             complement(91275..93188)
                     /locus_tag="PLF66_00450"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HMZ62529.1"
                     /translation="MKVLKKLILILFLPLLVLSVDFYFRIDTILQFNEKMRGYYLLSI
                     LASFLLYIFLFLFTSQISKRISTWISYLLYTVIGFIYTFSIVGSYGYYQKANMLPNYF
                     VIHYMIQEPLNSFTLLKDSFTFGSVITFLGLFIFSVALLSVFSRVTETFNRLGKIFIS
                     ILGIIVLSLITIISFNTRFQDQLYVSDINTIAFISRNIANRVISNQMGSTGLQSRTKI
                     SIKEKFPNSKMNLILVVAESLRRDHLSLYGYSRDTTPFLKKYAKEKNQEFVVFQNAYS
                     NASSTLISLPSILTGVSPIQSAADTHSYPLFWEYGKESGKKTFYITSHSLAWNNLGGY
                     FANGGIDYQFNRENSNEPIFNDIGIDDRKTLAAFDKHIRKLSEENKNFSGLLHLNTNH
                     FPFFVPSSSKVWQGNDVKDMYDNSVYHFDKLIESMFQTLKDTNNLENTIVIITSDHGE
                     SLLEHNYLGHIESYYLETVSIPMMVYFPKKLQTYDTFHNLQSNANKNVMNLDIIPTIV
                     HLLNKSLDSFHTVSFVGKSLMDSIDPDRVIVISNNNEISLYRVGISFIENNLHYIAKI
                     NSIPKQEELYNIKEDSTERKNLWSSIDSNQKHKLRDRLKDCVVCSEIFSNLQIEPPSV
                     KVSNHCGFRNWLF"
     gene            complement(93330..93857)
                     /locus_tag="PLF66_00455"
                     /pseudo
     CDS             complement(93330..93857)
                     /locus_tag="PLF66_00455"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            94548..96014
                     /locus_tag="PLF66_00460"
     CDS             94548..96014
                     /locus_tag="PLF66_00460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62530.1"
                     /translation="MKTFSSLKSILFLSLFFYTSVSIFAEEDTQIYTQKPIYSFPILD
                     SPFNTQRGSLQTENNSPVPSMEQSIHITKNITQLSHRALDSKLSESETINKNPILKRL
                     GILTFDFLFYSIPIPGNISWMHEEWHRAVMGRRGIASFNDVYVARPFSESISVSHVKD
                     SDLIQFKKDHPEEFVRLSAAGMESQTEMNLRMRKDTFFGGTASYLDTATLWINTTNNI
                     GYLQTCSNRSADKFTIKSNLEERNISKRDFVGLDCNAWVYDLFRPNEPYELRGIHPTG
                     IGIDRYIKYSNETNLNTRFQNFLLYGNGNHHSDLTKQEQDYINQQYKLSYLNLINPAL
                     IGLSRFTAINPFNGKEFFWSFGVAHYLTSFGYTIDLQFLFKQEKLNILFIYHTYANAS
                     LKLPGIEAEIIRYPLVVFGKETYINLGMILWLQPYQQKFWTTEKETGGIIKATLSLPL
                     FENLEVFFSPSLKSRGWVAGNVYLDKMFEFRSGVNFIF"
     gene            complement(96066..97076)
                     /locus_tag="PLF66_00465"
     CDS             complement(96066..97076)
                     /locus_tag="PLF66_00465"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HMZ62531.1"
                     /translation="MKKLFIGIFVSIIFIYFIGTKYDFQKEFSKFGDKISIAYLIPIT
                     VTQLIGLVIFSFRWRLLIEKKINLKHAISSSFIGYAANMVLPARGGDLFRVFYCRSET
                     DVQYFHLLSKLFIEKVIDFIFVILAGVIAFFIIGLNRPEQQSYAVFTVSGVIVLGILF
                     SLYLIRFQNEFIQIVSTKLFKLVGKETFYKDHIEVHLADLKDFLQVKKFIYPMIFTII
                     SWCFYFANYYYVNVMLGGELTYAEIMFLLFCGAMSLALPSAPSGIGVFHASIISGFIL
                     IGKDSNYGLLYATALHLISFVNITVTGLIFYLYWTYRRRHGKSGKLPGKSGKLPSDLE
                     NT"
     gene            complement(97120..97464)
                     /locus_tag="PLF66_00470"
     CDS             complement(97120..97464)
                     /locus_tag="PLF66_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008595386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HMZ62532.1"
                     /translation="MSVCIFCKIIDKMIPANIVYESENCIAFTDINPVAPNHILFIPR
                     KHIESVDKMSKADAPLIGNIILEMTEYARKNNFNNGGYRIVNNTGSDAGQTVFHIHFH
                     LLSGRKMEWPPG"
     gene            complement(97476..98366)
                     /locus_tag="PLF66_00475"
     CDS             complement(97476..98366)
                     /locus_tag="PLF66_00475"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HMZ62533.1"
                     /translation="MKKYLGIDVGGGSIRGTVVDKEGNSYFDFSVPTNPDSYNQEFLE
                     SVQTVVKKCISAYEVNGIGIGTPGPIDIDSGVIISSANLKNLSNVELTSFIRKEFHLP
                     VFFNNDANCASLGEYYFGAGKGSKSLIVFTLGTGLGCGWVLNGKVFNGFKGNGMEAGH
                     ITVVQNGALCGCGQRGCVEAYFSTKGFIGRYKDRTSIEIPNSKVFFEKVAAKEKEAME
                     VLDFGVTAFAEGIRNVVHLVNPDRIVFVGGITASYSLFGKVLEEKAKQIMFPVLSNYI
                     EFKVGSSLAGSYGAAALAFE"
     gene            complement(98380..98631)
                     /locus_tag="PLF66_00480"
     CDS             complement(98380..98631)
                     /locus_tag="PLF66_00480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62534.1"
                     /translation="MNDTSPEIESMLRKKYMSLSGWERVQIGSSMFDAAKKIVEYSLP
                     KNLSEKEKKIQMFLRFYGHEFSEVEKQKIIERITLNVSG"
     gene            complement(98624..99166)
                     /locus_tag="PLF66_00485"
     CDS             complement(98624..99166)
                     /locus_tag="PLF66_00485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62535.1"
                     /translation="MKNEIDIVRDVSKKLDSASIQYMLTGSVAMNYYSLPRMTRDIDI
                     VIQIGKEDISTIVNLFKADYYVEPDSIDIAIQSERLFNIIHDESFIKVDFIIRKSGNY
                     RLEEFERRKKIEFGDFQTFIVSKEDLILSKLLWSKKSKSEMQKRDIQNLVKSEVDLNY
                     LEQKLNELNLKDWYEEIMNE"
     gene            complement(99163..99888)
                     /locus_tag="PLF66_00490"
     CDS             complement(99163..99888)
                     /locus_tag="PLF66_00490"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002758855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HMZ62536.1"
                     /translation="MLHFLSNIEPKVAILAFFSILPWGFFLVALHPTKLQRKIVISFI
                     ALLLGVLSTELILRAHPIIWPEIDFKPKKNATILTQTVYISFIQAGMMEETFKELFIL
                     LFGLIAAFRYSSKEWQKEIVLIGGFVALGFSLIENYHYIGKDSSKVYTMFIGRTIFSS
                     NIHLLINLCFSLFLLKSNFQTQFRDKLIIIFYGYFLAVMQHGVVDFFLLPGSRFGNWL
                     STAMFVGIWVWVVRDLRTYVFRK"
     gene            complement(99889..101778)
                     /locus_tag="PLF66_00495"
     CDS             complement(99889..101778)
                     /locus_tag="PLF66_00495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HMZ62537.1"
                     /translation="MQDVIVYEQEPVEEKKRSFLNGFVFSCLSFLCWASPFLELGIFF
                     PIGVLVLYSHNTKLRVAAFQSLAFQVFIQVISYPIELYSLVNFDTEAYILTLKQEYMG
                     VIVAIWALMGLLFLFIEARLIIRRFGRLKLFREKNPIDTPMVVRPATPSEEWDNYEYQ
                     RVERSESENSEKPKKVISKKPFTVMLSSVLITWVISVFWFVFLFYFYAALNAETINSE
                     EISATIDKMLFQNGQVAIFLWLMTLYSTTGFLGRKGILNVLRRPFLSLYVHSRLARVP
                     TIDYFSEIYNRKKKYAKIRERILPGWGHIYLYRYWKGFPILFCFLLVFFFLVIALTFY
                     FDPMFGLNFLRSLGLKPGIHDKEFIPLTQNLALPIGLSFLLSIIYIYANSTLNASLKK
                     DGEPLSERGMQSGFNNNVYLSILAHLVIIAILFIVPERIQRSSSSKKKQDISKQHYQP
                     EKLEYYFIDPEIPDNVKDLNGGVISGTDTPSQKDGIKIPDQVVTDEGKVKGYVKRIKG
                     KKLPKTYSNYISARMRGPENFMEYWKRAPHPYSCVVAYTITTEGEIVDVILVESSAYP
                     DQDNLTLELIRSMSPVMPPPNVKGDVRVTELFWNGAIDPDAMPTPLQKDMVLHFDGRY
                     MEEEF"
     gene            101867..103258
                     /locus_tag="PLF66_00500"
     CDS             101867..103258
                     /locus_tag="PLF66_00500"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012476170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HMZ62538.1"
                     /translation="MAKDREDSLKQIVAVSKRRGFVFPGSDIYGGLSNTFDYGPYGIE
                     VLNNLKRLWWEYFVHRREDVVGLDSSILLNPTVWEASGHVGNFSDPLMDCKQCKTRIR
                     VDHFLEEKKGDGFASGKTLKDLQHVILEEKYPCPSCGSVGTFTEPRSFNLMFKTSHGA
                     SEDNSMDIYLRPETAQGIFINFKNIYTATRKKVPFGIAQIGKSFRNEIMARQFIFRTR
                     EFEQMEMEYFCEPGTQTDWFTFWVDYCMKWLTDVIGIKKENLRVREHAKEELSFYSDR
                     TADIEYQFPFGWGELWGIASRTDYDLTQHEKFSGVDLKYHDQVANKKYLPYVVEPALG
                     LNRLFLAVMSDAYAEEKLEDNETRTVLRFVSKVAPIKAAIFPLMKKDGLDVKAKEIYH
                     SLMSHWYVDYDDSAAIGKRYRRHDEIGTPYCITVDYDSLKDNTVTIRERDSMKQERIL
                     VADIPNYLLGKLK"
     gene            103998..104549
                     /locus_tag="PLF66_00505"
     CDS             103998..104549
                     /locus_tag="PLF66_00505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62539.1"
                     /translation="MNEFDRAVFVSNNYKEFGKDSKEDGFHSDLTQYFENVEIIYERD
                     FKNLLDLTEDMEYELQMYFDYLHENKIGFHCRMEDCNSMGIFDSKIRVSDGSLEYEDR
                     MQLKFFQLSEVPYSPKVKFVQSGRCKKCNSIHIECPECKNLLIDFEEDNILFCDNCQI
                     RFERAYSNRNNDEIIIPINDDIL"
     gene            complement(104747..105670)
                     /locus_tag="PLF66_00510"
     CDS             complement(104747..105670)
                     /locus_tag="PLF66_00510"
                     /inference="COORDINATES: protein motif:HMM:TIGR01784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HMZ62540.1"
                     /translation="MRIKLLPLTNDYIFKSVFGKHEDVILNLINSFPEFIGEGRIEKI
                     QILNPEIPRDGKFEKSVTLDMKAMDTKGNHFLIEMQATDRLNFSKRILYYWSKLYSKS
                     ISKGELYIRLPKVYSFNFVNFEMFKNKSKFYWHFQLIEKDDPQFLLTEDLSIRIIEIP
                     KLIGEFAELKTPLEYWVYLLKEASSLKGETMKALQKKNPKIKKAIQELRFVSLNKKSR
                     EYYEMKEKAEKDYGSMLAFRVQEAEKEAMEKGRAQGVEKGIEEGLEKGLEKGRREGRK
                     EGIKLVVEKLLAAGYKKKEISRLTGLRLKDI"
     gene            105959..106720
                     /locus_tag="PLF66_00515"
     CDS             105959..106720
                     /locus_tag="PLF66_00515"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002620012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HMZ62541.1"
                     /translation="MTKEFEGKVAIVTGTASPRGLGRSICNRIAEEGGNLVMVDLKQD
                     ACDAAAKEVSDKYGVKTIGVACNVTKLADCENVITKTKETFGKLDFLINNAGVLKDNL
                     LMRMSEEDFDFVVDVNLKGVFLMTKAASKLILKAESGRIVNISSISGLVGQAAQANYS
                     SSKAGVIALTKVAAREFSGRGVLVNAVCPGYVQTDMTGSLPEDIQAKLKEMVPLKRPA
                     TPEDIAGAVRFFLSKDSSYITGTILRVDGGGAIGM"
     gene            106868..108034
                     /locus_tag="PLF66_00520"
     CDS             106868..108034
                     /locus_tag="PLF66_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008590930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase domain-containing protein"
                     /protein_id="HMZ62542.1"
                     /translation="MREVSIIGAYQTKHANHADKHLRELVTEAGNGAIKDAGIDRKEI
                     QAVYVGNYAGNEFNNQNTMASYVSTILGIGNVPSYRLEGACASGGLALNQGFHAVASG
                     RFDTVLVLGVEKMNTQDPTKTMEIVSKGQDFEFEGKFCVSGPSGFALNAMRHMHEFGT
                     TKEMLSTVAEKNYFHGSKNPYAHKQKVVSLKNIMNARMVTTPLGFHDMSLVTDAAACV
                     VITTREKAKSLTKKPVSIKASAVGGDYFTLSMKKDSVSFPATVKAAEEAFKMSGLKRE
                     DIDVLECHDCFTITEIINIEDLGFVEKGKGGFYTLDGHTRLGGKLPVNTSGGLKAKGH
                     PVGATGVGQVVEMTFQLREESGERQVKNARTALTHVLGGPGAVAAIHILQKDFT"
     gene            108034..108360
                     /locus_tag="PLF66_00525"
     CDS             108034..108360
                     /locus_tag="PLF66_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002972400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HMZ62543.1"
                     /translation="MNTLKGKKCNSCNFLMLEPSFACPSCGSDSLSDIEFNGEGSIYT
                     FTVVNVGFGHLAAKAPYVLAIVELKEGLKVLTIVEDANLENVKIGDKVKFKHMDERQE
                     PIFQAA"
     gene            complement(108361..108717)
                     /locus_tag="PLF66_00530"
     CDS             complement(108361..108717)
                     /locus_tag="PLF66_00530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62544.1"
                     /translation="MKRIIVIIPFLFLSFVNSDLFSIDKTLEWSDYQGKHSWRDAAGK
                     CESIGMRLPTIWEFRNAYNSRLSKSWEEKGDSYWTYSEAARSFDDAYCFNIIYGEHSL
                     SYINVYKHVRCVRYSN"
     gene            complement(108774..109556)
                     /locus_tag="PLF66_00535"
     CDS             complement(108774..109556)
                     /locus_tag="PLF66_00535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62545.1"
                     /translation="MKANKALLLIIIIFIFSCSSKDAAKTDKPKGTRASAKGTVELSP
                     AQLTKGFVEGISFIPYLSKTKISTINDILNKTKLKVSLEVAYEYAYDKKLKEAKSDED
                     TGQVFKDMKVATEHFQKILKKYGLKNYSQYVLTAIDTDEDYILYATIERKLNFIKVSD
                     KKKADKILSLNPDDLKYYEPYEMDSSDNPLDTIIDFGAVSKKDAFTHKTQSILITMAA
                     NSVLEKKKSPNYWNVEKRWINGEAQSVMEESEANIEKRIGIK"
     gene            complement(109889..110440)
                     /locus_tag="PLF66_00540"
     CDS             complement(109889..110440)
                     /locus_tag="PLF66_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004767664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HMZ62546.1"
                     /translation="MTDVSYKGKILISNSSIVTDDFNKSVVFMVDHDATGAFGLVINK
                     KSSYLMSEVVMGLPDGIKDTFMYWGGPVDHGFISILHNNPNIHDPGLQVIPGVFLSRS
                     YDLLLKLLGSDDTAYHVFHGYSGWGAGQLESEFARKSWVSHDASPVNIFHPDPETVWR
                     DALRSKGGIYRYFAEHTKDPSLN"
     gene            complement(110441..111400)
                     /locus_tag="PLF66_00545"
     CDS             complement(110441..111400)
                     /locus_tag="PLF66_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008594689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HMZ62547.1"
                     /translation="MSDKLKIGIIGVGHMGQYHVNVAKQVQDSTLTGIYDADPERAKV
                     ISEKYSVPSFATPEALIEKVDAVVIAAPTFLHHKLSKLALTAGKHVLVEKPIAESVEQ
                     ARELVKLAEQNKLIFQVGHVERFNGAILELGKIVDTPMLIESRRLAPYNARIKDVGVV
                     LDMMIHDIDIVLNLVDSDVVKVHAVGQKIVSEHEDVATVVLKFANGCVATIAASRNTQ
                     AKIRTLNITDKNAYIKLDFGDQEIELHRQASSNTQLSAESIRYKQESIVEKIFVHKDN
                     PLKQEHEHFVKCILGKDSPQVEGEKDIRTLEIAHQILKEISSK"
     gene            complement(111397..112503)
                     /gene="dnaJ"
                     /locus_tag="PLF66_00550"
     CDS             complement(111397..112503)
                     /gene="dnaJ"
                     /locus_tag="PLF66_00550"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HMZ62548.1"
                     /translation="MSERSYYEILGVSKTATDEEIKKAYRQLAIKYHPDKNKGNKEAE
                     EKFKEATEAYEVLRDANKRRAYDQFGKAGVGAGGPGFGQGAYTDFSDIFGDFGGIFED
                     LFGGGGRGGRRSGPKRGSDLRYNLEITLEDAALGKEFKIEIPRAETCGDCKGTGASKG
                     SSPTTCPDCAGSGQVRHSQGFFSVSTTCGRCGGRGQVISNPCKTCRGEGLVEKKRTIN
                     IKIPPGVESGSRLKVSGEGEAGPNGGPHGDLYVVTHIKKHPVFERQGNDLIVTKVIPL
                     VMACLGGEVEVPTIEGKSIMMKIPEGTDPGQVFRLKGSGIPYLGSYGKGDQHVIIKVE
                     VPKKLTKKQRELLEEFAKESNESFGSYIKGKIFK"
     gene            complement(112507..114447)
                     /gene="dnaK"
                     /locus_tag="PLF66_00555"
     CDS             complement(112507..114447)
                     /gene="dnaK"
                     /locus_tag="PLF66_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017858734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HMZ62549.1"
                     /translation="MAKEKIIGIDLGTTNSCVAVMEGGDFVVIQNSEGARTTPSIVAF
                     TSKGETLVGQFAKNQAITNGANTVRSAKRFIGRRYNEITDEAGKVSYKVIRSGNDGVK
                     FETSSGEFTPQEISAKVLQKMKKTAEDYLGTTVTKAVITVPAYFNDEQRQATKDAGRI
                     AGLEVERIINEPTAAALAYGFDKKKASSKIAVYDLGGGTFDVSVLELGDGVFEVKSTN
                     GDTHLGGDDFDQVIMDWLVDEFKKQTGIDISKDKNTSQRLKEAAEKAKIELSGTTSTQ
                     INLPFITADSSGPKHLDMTLTRAKFDQLTKSLVERTRIPCINAMRDAGLSASEIDEVI
                     LVGGSTRIPAVQDLVRELFGKEPNKSVNPDEVVAAGAAIQGGVLAGEVTDVLLLDVTP
                     LSLGIETLGGVMTRLIDRNTTIPTKKSQVFSTAADNQNAVSVHVLQGEREMARDNRTL
                     GRFDLVGIPSAPKGVPQIEVTFDIDANGIVHVSAKDLGTGKEQKIRIESSSGLSEDEI
                     KRMIKEAEINAAADKKARELAEMKNETESLAYALEKTLNEAGDKVPATDKQLAQDEIK
                     RAHEAVASNDYDRIKAAHESISQIAQKIGANMYQQGGPEGTASAGGPTGGSAGASGNS
                     ANSGTGEKVVDADYTVVDDDKK"
     gene            complement(114473..115057)
                     /gene="grpE"
                     /locus_tag="PLF66_00560"
     CDS             complement(114473..115057)
                     /gene="grpE"
                     /locus_tag="PLF66_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016550519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HMZ62550.1"
                     /translation="MAEDNVATKENETNASNEAIGDENKVEVNEGKSELSSAQEELQK
                     AKKEIADLNDSLLRLRADFQNHKKRTAEEYTKIKKEAVKNFIIKLLNPLDNLERVGSG
                     SNLSEEMKPFVEGVGMILRELNGILEKENVQKLNPVGQPFDPTIMEAIASEESEDAKE
                     EIVTEVYQAGYVFTENNESFALRPARVRVTRPKF"
     gene            complement(115073..116104)
                     /gene="hrcA"
                     /locus_tag="PLF66_00565"
     CDS             complement(115073..116104)
                     /gene="hrcA"
                     /locus_tag="PLF66_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016551716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HMZ62551.1"
                     /translation="MELSPRHRMILKVLIDDFVSDNKPVGSKTLSEKYDIGLSPATVR
                     NVFRDLEEAGYINSRHHSGGRVPTERGYRIYVDNLISLYELSLKEKQRIQEEYLKNEL
                     KLEQILAVTCRVLSILSCSAGVVLAPKKNFDTLKHIELIHVNGAEVLMILVTRSGVVL
                     HHKLFFEDHVSQESLYKISRFLQDHIKGYDLDEIQNLFPALMIRNDAPEDFYKIADTL
                     QSAFLYDAGDNVDLYVDGLQNLCANFKDDDPDTLESVFSLLDDKKLLKEFFTKYISSD
                     GVSTFIGETNSEHLRGVSIVATSYRMGDKRIGSLGIIGPQRMNYTRALPLVDFTSKIV
                     SEMITKISK"
     gene            116835..117242
                     /locus_tag="PLF66_00570"
     CDS             116835..117242
                     /locus_tag="PLF66_00570"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HMZ62552.1"
                     /translation="MIKVLCIDDDAITLMLCTTTIKKSGLSSEIITLLNGQEGVDYYN
                     KLTSTIGYENHSFYPDLIFLDLNMPILNGWEFLDYFMEDHYPKLQKTKVVILSSTVDP
                     RDKEKAKQYPIVIDFFSKPITNEMLSKLSYKLN"
     gene            complement(117360..118046)
                     /locus_tag="PLF66_00575"
     CDS             complement(117360..118046)
                     /locus_tag="PLF66_00575"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HMZ62553.1"
                     /translation="MKRPKTLLFFGILFLTLPLINYIGLSIRFHYSLFQIKSILRELG
                     RIEIILLLFPMLVGIGLLLVKKWGWWLFLFYSIALILSNTITLFKDPHLYNIGALVQT
                     LLALSAIFYFLKKDISAPYMKMYPRGWRGEKRNPIQIQVRINSIDKLTRDISEAGFYL
                     DWKDDPFEINEEVRVEIETDSDRRFLSAGVVRKDEEGTGFAFRQLTDDDQIWIAKKIE
                     SVESRIEKRK"
     gene            complement(118216..119352)
                     /locus_tag="PLF66_00580"
     CDS             complement(118216..119352)
                     /locus_tag="PLF66_00580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62554.1"
                     /translation="MKKYILFWSFISVNILLFSYCTNHKSNQNIPLQILLLRASQNKV
                     NSSSGFLIKLPVTIGLNNISSNSSSGNNTILNNWDQIRQMPTFLKQVTDNIDNILAQF
                     YVNGILGKTSISTQSTDNFKIKFNPAFNGVISSNAYSGTKTFRYSVEIWNITNNLKVL
                     ELFYDDAIQRTSGKGSLLTFQPSAFDSSINANLKVECYTVTLDSQNTSMICSWVDGVF
                     KVSGNVDNVMIKTNFNLISNSYNMQLLARSNPNQGYVCAGGNSDFYSLAAITNVINGK
                     SLSVAKWGYNDNIIADTLCSNQNTLNYGYFDSTINSNVNNSSKYFVAQGISSDTNSYN
                     TGSYPLLQDTSNLFSQIGTGTNELTIQRITTIILSFKGNADPGF"
     gene            complement(119369..119935)
                     /locus_tag="PLF66_00585"
     CDS             complement(119369..119935)
                     /locus_tag="PLF66_00585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62555.1"
                     /translation="MFNQNIRKIANLILIFLFLSCSSKNDSSEKKKNKLLLASVLSYQ
                     QANTNKPVVDTTPTCSYISSGGIQTNVFLYTAATTTQSLKYAAEDLGFGTSLQSYSMI
                     RVTTKVNGKLTFTGSNGGGLFSIAVFSNVDPCAINARTANILTSAYTASSTTSSATLT
                     FNQANTFLVLSTISFNSNSPSISVIYSD"
     gene            complement(119965..121608)
                     /locus_tag="PLF66_00590"
     CDS             complement(119965..121608)
                     /locus_tag="PLF66_00590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62556.1"
                     /translation="MGFNTYYDGLNGWWDIDPTTNKCPMGGPFPSEIKVADWFLPVAN
                     QPPHFQNGKSALHSTLMIGGNDIMFNMETKGLLPTLNTYLVDFVTENITFMTDWHLEN
                     GKKVLLEGTIPVYSGAIDPYDSTIVNRQALCRPLAESFIKPQDIPWWACALGPQVCYA
                     MAKAAKDYDEKVAKAFTDFTKKVMRKKSTSADNFFDSINSMNLNNFAGNLGDAVTAGG
                     QALEQAAQDNIVHIIAVVGGTAVAMGTDNSAAGVATAAAIENELSKDKKAKAEWQASP
                     LLRLASINQACVNDKVTYDIGPAYKSAYPGYVEAFGLYNYFSNEWDFNFGNNFWVPKD
                     GIYDKYNTLLHDLLHKDRPLLIGDPIHIGPKGYELWSTLIGTKLKELGWNRTDDYIDP
                     IVNVDNDGPGKIIKKKSKDVGIFGQEATPTIRIKSKLEDSVNGYGQYGGYMRRYKDGS
                     AIYLSQLYDVPSADSGTKFGKPHVVTGLHQVRYDQLGGPTGKLGFPVTDKFCEALACS
                     VETVGFECGSISDNHLDFVQHIHEVIKAGCNPGYTVSVQ"
     gene            complement(121730..122197)
                     /locus_tag="PLF66_00595"
     CDS             complement(121730..122197)
                     /locus_tag="PLF66_00595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62557.1"
                     /translation="MKNILLKILIFIAFTIESLIAFPGDHNSLTCDQNFIDITLSPSV
                     ADLGGTFHSQIDEKLLDDYLRKSQTVLANNPAALANRARIIAAINADLNSSSSKIKQK
                     IKENHDSSNFEGHPLQVTKWGDSLSDFVSFYGVLTPGALGSRSYSTRWWDIRR"
     gene            complement(122220..122912)
                     /locus_tag="PLF66_00600"
     CDS             complement(122220..122912)
                     /locus_tag="PLF66_00600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62558.1"
                     /translation="MKKIILLLLLWIIFFNRCDSNIPIIGKKTEKDSLYSKVTQMCYF
                     ANLLGGPSGYKIVDNNDGTVALVNYSTLGVDGCPPPFAKRESILSIKYYIKKCIQGQV
                     YRSAQNDCKGTGTAANYYGAQKFQGCPTNNTACDDSSGNQSKTLSPARASCVNDKTVG
                     RNWDNLDFTLGTSIYNLKDYLFSRSDEIPSSSTDYYWMYDFGSRNISNTGGSPAGPLN
                     KNLYNYILCISS"
     gene            complement(122914..123999)
                     /locus_tag="PLF66_00605"
     CDS             complement(122914..123999)
                     /locus_tag="PLF66_00605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62559.1"
                     /translation="MKKKLSVILIILFTMNWMYLSESKAKWSGLNPVVGFFINPKIIG
                     FKSSKNQPLIAKEFLDAAGEWFQKGGYRFSFKFQGITLDKPASVEDIDCTARTDVQQG
                     DAVFFAEPNSDPDCTASSCVYLWSCNGSIVHADVQLNNSDFNWNHIGSDGDVKNLKTE
                     ALKAFGYMAGLGHCKTGDTVTDCQARTGSDPKEGSVMYKFPQIGRIDQIGSDEVTGIQ
                     TLYGTLNLPFPTSGKYSLTYGDMKSIQDFNSLNAIAGNTDEDARKRGGQFLDSLSDYN
                     FKRTKRTLPDQYDEFYNLVKMQTPTFDKEDLEHQRELLTQGIVSAVKLKEDALKGYHK
                     MDPNFIQSMIDRHLKLRNLTIDALEGK"
     gene            complement(124024..124695)
                     /locus_tag="PLF66_00610"
     CDS             complement(124024..124695)
                     /locus_tag="PLF66_00610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62560.1"
                     /translation="MEATPRGVTVSSDGKSYGGYYREYTDGSGIYLKLYTSNTPENTA
                     NVKYQTGAIHLPNKVNSVYRSNGGPEGKLGFPMTGTMCWGPGAFCNFKLTLFEKGFIQ
                     VDETPIIGGGVSVVTAVTDIPTAKINDPPDFDTCVSAACGSRWFEHNKVYLQPTSGKP
                     PFTFQIISGNLPPGFSLTPTGLLFGGPTNTQGSWTFQIMIIDSKNGDQQINSAVKTLN
                     FSTGL"
     gene            complement(124766..126322)
                     /locus_tag="PLF66_00615"
     CDS             complement(124766..126322)
                     /locus_tag="PLF66_00615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62561.1"
                     /translation="MKFLFLILVYFFLILEVISWPGDQDSLSCIPEQDYNNPRNRNDI
                     KAQYSSTGSFATTDEAKKIQNQKDAIERVKTIVRNNHDMNFRVTKLGDSLSDLVDFYG
                     VAIPRNIFPNYGDAAGTFGRISIFMDSWTDYMTAGAGILDPNAKLNFQNFGVSGFQTT
                     SVMTTLGINIDGTREDWTVNKDSPRCVRTRPMATGMVPVDYYEAITKEQSSLRSTLMA
                     GGNDIIQSGLGFNGWLPFLNRYQVDSTLLNIMAITDWHLENGKQIFLEGTLPVLSHPA
                     RNPYDTVLINRQTLCRPLNDLLVDLNEIPWYAAFIPGMMTSIIKWNEHVISEVTKFLS
                     DIYNPPPKPNTSSPGGFVNSANQALDTQLDKLLDEYLEFTANNNLVLSDAQKAELKRQ
                     IFERRTSPDNQYNDTPTRILFTSASIAQACVNDRIKNNLLPLYKQSFPKNVDGYPLYS
                     NFNKTGDIPYASNFWVPEDIVFRQTGIFFQGSGFWDAIHIGPEGYKRWGIQNCRSWLS
                     YSWRPKQYSR"
     gene            complement(126322..>126903)
                     /locus_tag="PLF66_00620"
     CDS             complement(126322..>126903)
                     /locus_tag="PLF66_00620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62562.1"
                     /translation="RNDDNKTALLELIGLSWGSKEIGTVVDNKDGTLNYTYQLLQTNS
                     WLGNKVISQKTILIRRCLIGQVYRSAQNDCQGTGTKDTLWGASQLQWCPTNDRSCEKD
                     GKADPTVSPAAKACATDTFAGKKWRLRSKDEGLRVLFSPSVTNDWTKIGFVSGNVWTL
                     ESNDNEKVFSAIAFESSLAYKNEAINILCTGEI"
BASE COUNT        42700 a        22128 c        20426 g        41449 t
ORIGIN      
        1 aaacttcatt tgaacgtttg gctcacccaa actcaatcat tgcttttgtt aagcaatgat
       61 aatgtaagct gagttagtta aatttaaaaa atagattcgg ttaaaatcta aataaaattg
      121 ctcttgctat tcattttgaa aaaataggat ttgcattccg aaaaatacat gaaacagtaa
      181 tggatgaaac tgattcgtaa gactattttt catattcgta aaacattaaa aacgggtcga
      241 atagcagaaa aaactgcaga tagcccgcta aggcatgcaa aagtgaatca agatacacat
      301 agtttctaaa ctgccatttt atatttttta attagattaa agaagcattc atttctatta
      361 taaccgtatc gcataacagt cgtaatccat atggttggaa aaaacgagga gagaaaaact
      421 aactgaaaat aaatcttacc tgctagatct gtttgctaat ttcttgttat ctttcaattt
      481 attcttttta acatttcata ggctagaacc tcacagtcta caaaaccccc tcttcttaca
      541 gataagaaat agaaaaagaa ttatcacacc tacatcgaca cagaaaattc catgtcgatg
      601 cattgcttga ttttaaaata atcttacaat tttatttcaa agaacttaag taaatccaaa
      661 gagattcaat ttctttgtca ttgaattctt ttaataacca aataggcatt ggaaatttca
      721 actcttgctt agaagaaggg gatacacctg tcttaatcat ctcacgaaaa gaatctaggc
      781 tataagaaga attagcacct aagcgaatgt tactcgcagg aggccaactt ggatctgctc
      841 cagggatagg tccaccttta taatccattc catggcaacc aacgcatgaa ttggcaagat
      901 actctccgaa ctgaatacca acctcttccg ctggtttatc agcaaaacct ttttgctgat
      961 caatcatttt tgctggaaat aaaacaggaa ttttattgag tgcggataga atttttgcga
     1021 caggtccgaa agtgttttcg tgacttggtt ttgtaacttc tggctgagag cgaatatatt
     1081 ttacgacagc cgctatatct cccttactta aactttgata gtcaaaggaa ggcataaatc
     1141 gtagagatct acccgtctta tgaacaccgt agcgaatggc tcgggcgatt tctttatcac
     1201 tccaattttt taaattgaaa ggagagatat tggctccgta gatcacaccc attgcaccat
     1261 tttccataat tttggcaccg ctcagatctt ttccgtggca gtctacacaa cctgctcgga
     1321 catggtaaat tctttttcct agctctagat ccgctttttc tacttcagtt gtgatttcaa
     1381 aatcgagaga agagtaagtc ttactctttt ccatctcaaa acgaatcatt acaaacgcgc
     1441 aaaaacatcc tattacaact agaaatccga ttaaaacttt cttcagaatt gaaaatttca
     1501 ttctctagta acctcatcta tatattgttt cattcacctt atgcaagtta agcatagcat
     1561 tcaaatgagg cttgctttta gccctccgcc ggctattgac atttttactt tattagaatt
     1621 ctctaatatt ttcaaaatcc tcatatattc ttaaattccc taacagagca taatttgatt
     1681 taatcaaaaa ttgaattaga tatgtttttt gtaaatacta aaatgcgaat tgagtctcaa
     1741 atacgatatc cgacagaata gagtatatgt atcaatgttc aaaactctgt ttgattctat
     1801 tttctaaagt tctaaaggga actacttgat gaaaaaaagg gtaaaaccca attgcccttt
     1861 cttctctctt tcgtacctag caaatctctg ccaatccttc catttttcct aaaaaggaag
     1921 taatcgatat tttaaaaata agcgttgaaa ataactgact tattagtcat ttattatttt
     1981 tttaaataaa agaatcattc aggagaagaa aattaataca atcaactttg aaaccgccga
     2041 cgagcattgt ataaaaatag atgattggaa gattcactat atagaggcag gagaatgaaa
     2101 atgcatagcc ttaattcata cagcgggaca tagcctagaa agccgacaat cgttaaggca
     2161 atttgctcaa aaaaattttg aatttgaatt tgattcatat attcaatcat cactgcagta
     2221 cagccgatgc aatctaatgc gtttttaaaa atcatgatca tcgtattgtc attttcctaa
     2281 aaatccacat aatcttagtc aaaaacatca tcgataaaca cctccggatt ttaaattaaa
     2341 ttaaataaat tgcgcagaca gactttatag cactaccctt cagcctatgc agggtttctg
     2401 attaatgtct gtgatatatt ggagttcaga ctgtgccgac aaagttgaca ccgttaacta
     2461 aatttgtata atagacatta accttgcgca gctagcgcag ctaagaattt ctgaatttcc
     2521 tctgcctaga caaagcacgt atcttatgtc acttgggcaa tgggcaagcg gtctcattca
     2581 agattgaagc ctgctctttc gctaaacgtt tatttttgac ttcattctat aacgtcttga
     2641 attcttcagg agtcataatt actcctactg ttttttatac ccaaaaatct ttgataaact
     2701 tttaatcggt agtcgcgccg ttatattttt ctaccagatc agccgcttta gcgacaatgc
     2761 ttttgatttg tttcaccatt ttttaaggct aggaaaacgt cagcgatgca tatgacgcag
     2821 cactttatcg gtaaccattc atttacccac cctaaaaatc tcgtgaacaa aaaccttcat
     2881 gcataatgta atcatcggca caaccattaa accagtgcgc tctccaatca gcgtcgataa
     2941 aatttacccc ttttagttca agagacctta ttatgaaaga ttcgcagtcc ttttttacga
     3001 tacgccttta acttgtaaga cagaaatggg agacgattat ggaagtagtt ggatacaaat
     3061 tcctagaaag aaatttagaa tgccactctt taatatgaac gaagaccctg agtatacttg
     3121 tgattatttc acagatgcaa gtcacatgtc tacgaaatgc ttccctgatt aaactgatta
     3181 tatttttaat aaaataatca aaattaagaa gaagtagtct tccataatta acgctctaat
     3241 tttaataaat ggatattttg gggagcgata agaaaattat accaagatgg aaaaggagag
     3301 aattaaaatt tttatactct tcttggttta tatagatggt gtagctaaac agcaaaaata
     3361 ggagtctaat atcagaacta gcaaatgttc taggaagaaa aattagtata ctataaacta
     3421 gcttatagaa tgttaaaaaa tacaggataa tcaactagat tttactttta cctttgggta
     3481 tagattccaa atcaaaaata tttatctgca aggatttagt tggtattagt tcaatgtgct
     3541 actgaatgta ttttctgata ttagaaaaat acgtttttag ggcgagcagt aaaatagtat
     3601 tagaatgact gaggttaaaa atgagcaatg aacaaattgt aattgatatt ctacaggaag
     3661 gaagtgtagc tataatctat gtgcgggggg atttaactct tctagatgct cctcaattta
     3721 aaaaaattgt tgatgacgaa gtcaacaaag gcataaaaaa acttttgttt gattttgaga
     3781 atttaacatt tcttgactct tctggaatag gtctattact ccgctttcat gcacaactaa
     3841 gaaaactaaa tggaaaaatt gcttatatta atttccataa aaatgtagaa aatacaatgc
     3901 aaaaggcttt acctcaacag ataagaagta catccttttt tgaatcaaaa gagaaagcaa
     3961 tggagtatat gaattcataa aaatatttaa attatttaca ttcaacatag gtataaaaaa
     4021 tcatttaaga aaaaacaatc tagaaaaatc agatttctag attgtttttt tgactaacgt
     4081 tttttcggtc aaactaaaca gtcttggttt tgacgattgc tttgttccaa ttttgaattt
     4141 ctttttctct ttgctctgct ttcatagtag gcttaaattc tttaaatttt ttattcatag
     4201 attttaaact ctcaacggaa gaaaaaactt ttgattctaa tccagcaagg taagcagcac
     4261 ctagaacagt agtatccaca ttatccggtc gttgcacttt cttaccaatt atatctgcct
     4321 ggaactgcat caaatactcg ttagtagttg caccaccatc tacttttaaa attttcagcg
     4381 ttttacctgt atcattctcc attgcactca ctaattcata agactgaagc gcgatagatt
     4441 ttagagcagc tctaacaatc tgagcttgat ttgtatcacg ggtaattccg tatatagctc
     4501 cacgggcatt catatcccag tgaggtgccc caaggcctgc aaatgcagga acaaatacaa
     4561 tgtcatcttc gttttttaaa gtttttacaa ttttaaaagt gtcttttgaa tctttaaaga
     4621 attttaaata atcacgtagc cattgaatga cagcacctcc aataaatacg gagccttcta
     4681 acgcataaac agtattaccc tctggtccgc aagcaagtgt agtaattaaa ccattcttag
     4741 aaatctgaaa atcgtttcct atattaaata aaagaaaaca acctgttccg tatgtattct
     4801 ttgcttcccc tacttctgtg cataattgtc caaataatgc tccttgttga tccccaacaa
     4861 gtgctgtaat tggaattccg tctggcaatc cctttactcc ttctgtctta ccaaactcat
     4921 aacgagaagt atgtgtttct ggtaatatag aagcaggaat atttaaaata ttaagtaaat
     4981 ctacatccca tttcttatcc ttaatattat aaatcaaggt tctagaagca tttgtgtgat
     5041 ctgttttatg cgactttcct tttgtaagtt tatagagcaa ccaagtatcg atggttccaa
     5101 aaagcaaatc acccttattc gctttatctc ttgcaccatt cacattatct agtatccatt
     5161 taattttggt gccgctaaaa tacgcatcga gtacgaggcc agttctattt ctaaaatcag
     5221 actctaaacc ttttgctttc aaatctgtac aaatatctga agttcttctg cattgccaaa
     5281 caattgcatt gtagataggt ttgccagatt tcttttcaaa taaaactgtt gtttctcttt
     5341 gattggtaat tccaatacaa attgcattct ttggatttag ttttcctttt ttaattgctt
     5401 gaagaattag cttctctgtc ttctgccaaa tttcagtggc gttatgctcc acccaacctg
     5461 gtttaggaaa aatttgctta aactcctcat aggcaactga aattaccttc ccttttgtat
     5521 caaaacaaaa tgttctaatt ccagtcgtac ctgcatcaat tccaataata taattcttca
     5581 attctgactc ctagttaatg ttttcttaaa aataaaatta tttctttgtt aagcatgtaa
     5641 agaataaaaa aaaaatcgaa tgaaattcaa tgagcaagtt agacattgaa tttcaattaa
     5701 ttaaaatttt aaactgtagc cgaccgtaaa atgaaaaata tgatgaacaa atttttgttg
     5761 ttgataattt tgaataagat gtgctttaat tgcctcccgc ccatatccat tcggatctgc
     5821 accagaattc gcagcgctat aattgtctac ggctctagta attgaatcga caactatctg
     5881 attttgtatc ccatgaactt tggaaggatc ttcaactcgt ccatctctat gattagtatc
     5941 tttgtaaacc ccggcacttc cgaatccagt agtcgtatcc cataaactag aatctggacg
     6001 tatcatatct aaaacactaa agtacatccc tccataatta acttgaagct taggtgtaat
     6061 atctgacact ccgctcctat agtctgataa attattatgg tattggtaac cagctgctag
     6121 gctagcagta aaattaagtt ctttatctgg gaaaaattta tggctaatgg ataatcgatg
     6181 attgctagtt ccattccctc caccaccgcc ttctgaagta acacgataat aggaagtata
     6241 tgttggactt attgctttga gaaaaggaaa ttcaagtcct gtgacaagtt cacctaacgt
     6301 aacattataa ttatcattat tcgcgatata ggctcctgtg ataacttttc caaacctagt
     6361 attatattta taagtaaacg atgcatcaaa aatatcttta agaccatttt tctcctttct
     6421 cattcgaata gaggatggat cttttggatt agctttatct cgtgtcaatg catcgttaat
     6481 taaaatattc gggtttcctg ctagtgctga ttctaggtat ttttgagaaa aatcagtttt
     6541 atcacctgac gctgtttgca taaaataatc gttatcccta ttggctcgat gtgctgtcgg
     6601 actataaaca ttcattccaa tgctaatatt ttcagttggg ttgtaagcga ctccggttac
     6661 gacattccaa gcttgtggaa tagaatcata agccatatta tcacgctggg aaagtttatt
     6721 gcctaatacg ctaaaaccac gaaaccaaat atcgttggaa aaatactgat taaatgtaat
     6781 cggattgact gattcttctt tagcgggagt tgtcgaggcg ggatttacaa cttctgattt
     6841 tggagagggc gatgtttttg gttcttcttt taatactgac ggttcttgtt tttcactttc
     6901 aaccatattt gttttctctt ccattgccaa aggttcttct gctttaacag aaatattttt
     6961 aacttcttct ctttcgtttt tttctgcgat ttgagacttt tctttcgaaa tcgatgtttt
     7021 gatggatggt ttttgtttct gctttccctt tttagttttc tgtgcttgca atgatactgt
     7081 cacagaaagc gaaaaaatta taactattct aaatatgtta aagattctca ttcactactt
     7141 acctacactt aatggttttt ttcttttgat actaggttta caaaatcatt ttctgagatg
     7201 tgagctaata aggttcaaaa gtaataacca ctagattatt aaatcgaaga gaatgtcaaa
     7261 tagaaagaga ggataacaac gcacttaaca aaaaaaatat ttttttttta atttttagcc
     7321 aaacatttta gtcgaaggtt tttttattga ctcaaaaata tttctacttg tgaatgacac
     7381 ttatctccga actaaaaaac tatgaaaaca cctaacatca ttttgcctat attctattta
     7441 ttattgatat tcctattccc tatgtgtaag gaaaaaaaag aagaactcat ttttgaatcc
     7501 aaggataaat acgatgccgc aattttaaat tgggtagaaa tttttcagcc gtcaactctc
     7561 aacaaacaag aaagattaaa tgaacttctc tggttcaaaa atgtttcaaa agaatttaga
     7621 ggaaaaaaaa taaagaccgt tgccgagaat attgacacgc attattggga aagccgcgta
     7681 ttaacaaagg ctttttatga cattactggt attgaagttg aacatgaaat tatcggcgaa
     7741 ggagatgtag ttgttcgcat catggatcag attgaaaatg gaacacaatg gtacgatgca
     7801 tacgtaaatg atgccgacat ggtaggtaca catttgagaa ctcaaggtgt ggtcgtgtta
     7861 agtgattata tgaaaaagga aggtaaggat tttacgaatc caaatctaga cctagatgat
     7921 tttttgaatt tagaatttgg ccaagactat gatggcaatc aactgcaact tcctgatcaa
     7981 caattcgcta atctatattg gtttcgctat gactggttta cacgcgagga tataaaaaaa
     8041 caatttaaac agaaatatgg ttatgaacta ggagttccaa ttaattgggc ggcttacgaa
     8101 gatattgcgg agttttttac aaatactccg attgatggta aaaaagtata tggccactct
     8161 gattatggtc agcccggtgc atctttaggt tggagattca cagacgcttg gttgtcgatt
     8221 gctggtgtgg gtgacaaggg tttgccgaac ggacttcctg tagacgaatg gggaattcga
     8281 gtcgaaaaca aagtgccagt tggctcctct gtttcaagag gtggcgaact aaatggacct
     8341 gcagctgttt acgctcttac aaaatacatt gattggatga aaaaatatgc tcgtccagat
     8401 tcatttgaat tggattgggc aacacacggt ccaactgcct ccgagggaaa tgtagcacag
     8461 cagatattta tgtatattac atggttatcc gatgcaagat ttcatagtgc tgatagtcca
     8521 gtctgtgata aatatggaaa acctaagtgg cgagtagccc ctaccccaca tggaaaatat
     8581 tggcaagaag ggatgaaagt tgggtaccaa gatgcaggaa gttggaccat tccgaaaaat
     8641 gttccaagtg atcgaaagaa aatggcatgg ctttgggcgc agttcgcagt ttcaaaaaca
     8701 gtggacttaa aaaaattctc gataggtgga acccctattc gtaaatcaac catccattcc
     8761 gaatatttat caaagcgatt agaagattac ggtgggctaa tcgaatttta ccgatcaagg
     8821 gatgaaaaga cttggacaga tagtggacct aacgttcctt actactcagg gttatctgct
     8881 ctctggtgga aaaatgtagc aaaagcaatt actggagaat ttacaccgca agaagcaatg
     8941 gataatttgg cagaagagca agacgaactc atgtctaaaa caaaactaaa tgcttacgcg
     9001 ccaaaactaa atccgaagaa agacccaagc tattggtttg cgcaacctgg atcccctaga
     9061 ccagagatca aagaaagaga aactcctaaa acaatagcct acgaagaact tttaaaacaa
     9121 tggacaaagg aaggcaaaaa atgaataccc gtaccaattt cgaaaaggta tttacctttc
     9181 atagatttac taaattatct ctccgaaaaa aagtaaattt tatcggtata tttattatac
     9241 ttcttttttc agtaattctt tttgtaaaag tccttccgct tttagaagca ggaaaattag
     9301 aagaaagacg aggaaaacta agagccgtag ttaactcagt tgtttcctta attgaccatt
     9361 atgaaagagg gcttcgaaaa caatcttgga aaacagatcc gactcaaccc aaaaacattg
     9421 aagaagcaaa gaatcggata attgaaaatc tgaaatacat gcgttatgat aaaacagagt
     9481 atttttttat tttagatgga aaaggaaaaa tggtcatgca cccgctcaag cctgaattag
     9541 aagggcagga tatgatgaac acaaaaggtc cgaaaggaga agctattttt catgatttgg
     9601 ttcttcaagc tcaaagagat tctgaagcat ttgtaagtta tacttggcag tcgaaataca
     9661 gccctctaat ttttgaacct caaacttcat atggaaaata ttactgggca tgggactgga
     9721 ttgtttgttc tggagtttac acacaagata ttttagattc tatgcaagag attaattatc
     9781 tatccgccgg ctacgtagta gtaacctctg catttaccat ttttatttta tttatttttg
     9841 tatactttaa tctatctaag ccgcttatga acctcttaaa agggattgaa gagattaaaa
     9901 aagataactt agatcatatt gtagaagcac actttgatga tgagctaggt catattaccc
     9961 gccagttcaa tgcgatgact gaaaacagaa aacgaagtca agaagaacta attcaattga
    10021 atgcttctct cgaaacgaag gtaatacaaa gaaccgctga actatctgaa acattaaaac
    10081 aagtacaaga attaaaggtt aagcaagatg gtgattattt tttaacctct cttctcttat
    10141 ccccattaca atctaaaagt gctgaagaag aaaatgttaa gagtgaattt tttattaaac
    10201 aatataaaac tttttcattc cgaaattgga atgcagaatt gggtggagac atttgtatta
    10261 ctgatagcat caaactaaac ggtcgtaagt atttattttt cgtaaatgca gatgcgatgg
    10321 gaaaatctat tcaaggggct ggaggcgcat taatttttgg aacatctgtg aaagcagcac
    10381 taattagaat taagttaggc aaatccccaa atattttacc ggaagcatgg ctaaaaaatc
    10441 tataccatga tttagatgag ctttttacta ccttcgatgg ctctatgtta atctccgctg
    10501 ttatgggatt aattgatgaa ggaaatggat tagtttattt tttaaatgca gaacaccctt
    10561 ggcctgtact ttatagagat ggaaaagctt cctttataga aggtcaaaat caattaatga
    10621 aaattggaac cccagaaaag gaatacaacc tgttacaagt taatattttt caactaaagg
    10681 atgatgatac atttatcgca ggctctgacg gaagagacga tataatattg attggaaccg
    10741 atgatgtaaa tcatgacgaa acattatttc taaagatagt agaaaaggca caaggagata
    10801 ttaatgagat tgtggaagga atttctgaaa gtggaaaatt aaaagatgat ttatctattc
    10861 tttcaattaa gtttaacaaa ctatctacag aaaataactt cgatttgtct acagaagaaa
    10921 aaaagatttt actgaaagca actaagctac ataagaataa tgagaacgag gaagcattag
    10981 cattgctaaa gagaaacttc gatcataagc atttttctat tcctgttttc aacctaataa
    11041 aaagaattta tttagtccag aaagactatc tacaatattc taaacttcta attcgttacc
    11101 atatgctcca tccagaaaaa aatgagaacc ttctaaaaat ttcgaaagcg ttagttctat
    11161 gtaatcaact agatcaagct gcagactatg cagaatgctt aagagtaaga gaacctcata
    11221 acgaagataa cttacttcat cttgcaaaga tctataaact atcttctcat tataatgttg
    11281 ctcttcgtcg attaaacgaa gttttagaat taaatccatc caatgaggaa gcatttaaac
    11341 tcaaagaaaa tattgaatta ttaattcaca aagcaaatat aattaatttg gatgaatcgc
    11401 tttttgaaat ttcaaaattt gattattaga ttcaattttt tttgaatcta ttatttttag
    11461 tcgtaaacat agctatggaa gtgtgggcaa tgtttttaaa attaccaaaa atatttatag
    11521 attaccgctg atgaatagag ataaacttgg cggtaaacaa tgattagctt cccatagaat
    11581 agaccttaga ttgagctata ataaaagttg gggagatatt tagaatagtt attttcgttt
    11641 attaggcact ccctaaaaac tcggtatttt gacctaaata gcaagaatga attcatacca
    11701 tttcattctt gactaacaaa atcttcgccc atctacttca aaataccttt ttttgaaaga
    11761 gtcgcaatcc ttttttactt taagccattc acttgtaaga tagaaatggg agaaaattat
    11821 tggagaagtt ggatacaaat tcctagaaag acaaattgag tctcaaatac gatatccgac
    11881 agaatagagt atatgtatca atgttcaaaa ctctgtttga ttctattttc taaagttcta
    11941 aagggaacta cttgatgaaa aaaagggtaa aacccaattg ccctttcttc tctctttcgt
    12001 acctagcaaa tctctgccaa tccttccatt tttcctaaaa aggaagtaat cgatatttta
    12061 aaaataagcc ttgacattaa atgactaatc ggtcatttat ttacaattga ttctttctag
    12121 caaatttttt taaataaaag aatctttcag gagacgaaaa tggatacagt caactttgaa
    12181 accgccgaag agcattggat agaaatagat ggtttgaaga ttcactatag agaagcagga
    12241 gaaggaaaat gcctagtctt aattcatgca gcgggacata gcctggaaga ttttaattct
    12301 atcttcgata aattaaaaac tagttataga gtcattgcaa ttgatttacc aggtcatgga
    12361 aaatcggatt tatttgatcc acctacattt gcaaatgtta ttactcttag taaatatatc
    12421 caagaatttt ctaaacaagt agtgaaagaa aaagcaatct atctaggaaa tagcattggt
    12481 ggttttttat cgataagtct tgcactcgcg agtccagaac ttgtaaaagg acttgttctt
    12541 gtgaattcag gtggatttaa tgagcctggc ttgattgctg ataatttcac aagactgaaa
    12601 ggcaaggaat ggattacgcg aatcgtttgg aatacttttc caagtctgta tattaaagtt
    12661 aaaaatgaat tcaccaagga cattctttct aggatacgga ataataaaac agacaatgct
    12721 atcaagacta acgcatcgat ttggagaagt tttcttttac cagaacataa tctaagagaa
    12781 agagcaaaag aaattgaatg tcctactttt ttaatttggg ggaaacgaga tcccgtaatc
    12841 gaattaaaaa taggaatcca agcgacccaa ttgattccag atgcaaggtt agagcttctg
    12901 aatactgggc atctaccatt tgctgaagat cctgaaaatt ttttcacagt attaaaatct
    12961 tttttggaaa caattgaatg aaaacctctg gacccaaaac aaaagaagca ctcatacaat
    13021 cagcacttac tctcttcgaa aagaaaggca tttctaacgt tagtgtagat acaattacta
    13081 aagcagcaaa tgtagctaaa ggatcattct atacacattt taaaaataga gccgattttt
    13141 taatctactt acacagagat tttcatgaga aattaaaact ggaaattaca aaaggaatac
    13201 agaatcaacc ttttggaatg gaaagacttt ggataggaac agagacctat ctcaattact
    13261 gcttatcaaa ttcgagtatg aaatcttttt tatttgaagc gagaatagag ccagctttaa
    13321 caaaggaagt ttcgaatcgg aatagagagt tttctatttg ggcagaaaag gattttgcaa
    13381 aagcaggctg ggagtatacg caagcaactg caagtctgta tgttggaatg attgcggaaa
    13441 cagccattca ggaaatgaaa gccaagaaga aactccaaaa cctaagaaat gcgattgcac
    13501 aatttatcga ctagactttt ttacaaaatc ctcaaaaaat ttttaaggat aatcattatt
    13561 atcctttatc aatcagagtg aattattttt tcttatgttc ctctagcatt tggttatctt
    13621 ctcctaggag agatttatgc tcttctatca gtttttcatg ttctgcattc attgattcat
    13681 cttcttttaa aattctctgg tgatctttct ctacatcttc ttttgaaaga gaaccagata
    13741 gatgtttctt ttcaagctct gcatgttcat caattagttt tttgtgtttt tcaatcacgg
    13801 ctctatgagc ttctaaaatt tttccatgaa gaacttcttt ttcctcatga gctttatcat
    13861 ccccttttcc tttggactca ttgtgtttct ttataaacgc ctcatgatcc gcttctaatt
    13921 ttgcatggtc agcttccata cttgtatgtt ttgcttctaa tacttcatgg tctttcttaa
    13981 atttcaactc aagagaattg tctttgcaat tccaaagaat tacaagagcc attgccaata
    14041 tactgatttt tttggattgt ttcatatttt gtccttacta attaaatgta tattcttaaa
    14101 tctgagttaa aatcaattac cacaaagtct agaatataac aaagtctata actagtgaca
    14161 ctttttttgt caaatagatt ttaaacgtta tgcaacagag acccatttta tgatttaata
    14221 aatcatagaa gcttattgta gaaagaattc tcatctaaat tattacaaat attacaaata
    14281 aaatgattct aactataaaa aagaaaggaa ttagattgca gaattaagaa agaattttga
    14341 aattgaaaga actctcactc aaaacaatga acttaaacaa gatacaaagg gaataaaatc
    14401 tatgtcagat aaacttaatt caaggctttc taccattcgt atattgcata ttactctaat
    14461 catgcctcct attgtgctct atggattttt tttcataaac caactcattt ttattgaaga
    14521 tattgtctct aaaattgtag gaattctaat cgctgcatcg agtgtgatta gttatgagtt
    14581 ctttttaaaa cagagcttta tatctagata caaatctaaa actttcgaag aaaaactaat
    14641 cggataccaa tctctcaaaa ttctacaata ttctatgatc gaaggtgcgg ctgtattcaa
    14701 tgtaatcctc gctgccggag agccaaatcc catgaatgca ccgatagtcg ccttactcgt
    14761 tctttattat attctttttt taagaccttc taaaagtgaa tttctaaaac tcttccaatt
    14821 aaaagaacat gatttactca atacacaata aatatacaca aaggataaaa attgaaccct
    14881 tgaatcaata aatcattcta ttggatcaat cattcagaag ttttatgagt ttaatcagtt
    14941 ttattgaaaa ttctttttta atagcgggcg gcttttttgg agtcatttgg ggcttacaaa
    15001 ttttaattac taaaaagtat tattccctaa gtcttgtatt atttatctcc tccctttggc
    15061 tctttgcagg cgcttatagt ttttcaggaa tctatctaca atatccaaat ttatttttac
    15121 ttcatttgcc ctttgtctat tgcgtaggtc ccctacttta ttttttctat aaaaaaaatc
    15181 taatggatga agaatggaaa acattttact ttcattttat ccctgctatt ctcgtatttc
    15241 ttgcgatcct accttttcac ttctacgaca cagatcaaaa atatttattt ttaattggta
    15301 taaaagaaca aaaaaataca atttactctt tactgattct atttctaaac tttggaacca
    15361 aagttcatcc gctcatttat ctcactcgaa tcattttaga aaacaaaacc tttttcaaag
    15421 aacaaagttc taatctttta tactttatta tcatttgctc ctctatttac ttagatttat
    15481 gtataggaat tttgggattc ttatttaaat cttttttcct aattaaatta agtgcgtggc
    15541 tacttccgat taacctttgt atcttttttt tattagcctc caagtatcca gatatgcttg
    15601 aaatggtcaa aacagaattc aaaaaatcaa agtatagaaa ttctaagatt caaactctta
    15661 acctagaatc agttctctcc aatttagaac aattgatgaa tcaagaaaag gcattttcgg
    15721 atgaagatat aagccttaca tctcttgcaa atgaactttc gattacccct catcagctct
    15781 cagaaatatt aaatgaaaag ctaaataaga attttgctca atatatcaat gaattcagaa
    15841 ttgcagaagc aaagaaatta ttattagaag aaagcaaaag atctatctta tccatcggtt
    15901 atgcagttgg gtttaactca aaggcttcct ttaaccgagt ctttaaaatt acgacaggtg
    15961 ttacgcctgc agaatttaga aagaactaca aagtctcaaa ttataatcta acctccaatc
    16021 acaagtctca ttctataaac tgagacgaca ttttttttct cctctgctaa gataaatgca
    16081 ttcaggagaa aaaattatgt ttaaaaattt agtagaaatg atttactaca gcgcaaatcg
    16141 atttggaaac caaggagcat ttgctactcg aaataaaagc aaaaagtttg attcagttac
    16201 ctactctgaa ttattggaaa tgtcagagaa cctagctctc gccttgattg aaatcggagt
    16261 tcaagcaaga gagcatgtag gaatcatagc agacaatcga gtggaatgga ttgtctctga
    16321 tatcgcgatt caaatgacag gtgcagcgga cgttccaaga ggaaccgatg taacagacaa
    16381 tgacatttta tatattctgc cacatgcaga tgtaaaagtc ttatttgtag aaaacaacca
    16441 gacactcgaa aaggttttaa aattaaaaaa taagttaggg ctaacgaaga ttatcctcat
    16501 ggataaagat gccaaacttt cagaaggaat tgtaaatatc tatgatttaa tcgaaagagg
    16561 aaaaatactt aggaaaacca aattctattt gttgcaagaa agaattcagg gcatcaaaga
    16621 agaagattta tttacattga tctacacttc cggaacaacc ggcgctccga aaggagtaat
    16681 gctcactcac aaaaatatgg tttttcaaat caatcatctt ccgatttcac tttctcactc
    16741 agaaagaatt ctatctattc ttcctgtttg gcatattttc gagagagtct ttgaaatgat
    16801 tgcaatcgct actggttcct gcacctatta tacaaatatt cgcaatttga aagaagattt
    16861 acaaattgta aagcctacct tcatggcttc tgctcctaga ctttgggaaa gtatctatca
    16921 agggataatg ggaaatttag acaaagctcc taaatcaaaa aagaatctat ttaatctagc
    16981 ttataccgtc tcaagagttt atcatgattc tcttcgtttc ctattgaaac aaaaaataca
    17041 attagaacct agcaatcact tgttctattt ttttatgagc atttatcacc tatcggtttt
    17101 atttatgcta tttattccaa atctaatttt agataaaatt gttttgagta aaattagaca
    17161 ggcaacagga ggacaattgc gtggatccgt ttctggcgga ggagctcttc cgactcatat
    17221 agatgagttt tttaataata taggaatccc agttctagaa ggctatggga tgacagaaac
    17281 tgcccctatt atttccgtta gaacatttca aaatttaatc attggaactg taggaaaggt
    17341 attccctcaa acatcaattc gaattttgga tataaacagt ggaaaagaaa tctttccggc
    17401 aaacaaaggt tatgcgatca aaggagagat ccatgtgaag ggggatcaag taatgagagg
    17461 atattacaaa aatccggaag ccacttccaa ggttttaaaa gacggttgga tgaatacagg
    17521 agatttagga gttatgacat ataacggttg cctgaagatt gttggtagat ccaaagaaac
    17581 cattgttctg ctcagcggag aaaatgttga acctgttcca atagagcata gacttcttca
    17641 ttcagaattc atcgatcaat gtatggtaat tggacaggat aaaaaatttt taggggcttt
    17701 aattgttccg aatctagacc ggttcaaaga atatggacaa gactataaat caatcgcaga
    17761 aaataaatat gtatatcaaa aattaagtga ggaaataaaa aaagcaatca gtgcagagaa
    17821 tggatttaag tcatttgaaa aagttttaga tttcagatta ctaccaaaac aatttgaagt
    17881 tggagatgaa ctaactgcaa aactatccgt taaacgacat gtaatagcag agaaatacca
    17941 acatctaatt gattccattt acaagtagtt taaaaattat aataactggc tggttctaac
    18001 ttacaaattc caccttttga agctagattg attaaatcga ttcctttaaa atcgcattta
    18061 tcaatcggca atattgaaat taaggtttcg cacttctggt agtctttttt gtaatagatt
    18121 acctttttgc gatcggattc agctaatgta ttcgttttaa attgcttttc cacaaaagaa
    18181 aatatatcta aactcttggc tcttggatca cctcccgagc tagagcattt taatatagtg
    18241 agtaaataaa gacgggctct cgcatcgctc tcaggtatat aggttggacc atccggagtg
    18301 caagctataa ggaataaaaa aataaggaaa attgacttca cgattgttag acgccccttt
    18361 actttgactt aacttctaac tttcgatttt tttttataaa ttccttggga tttaaatatc
    18421 cagcggttaa cactgaatag ccgtgaccaa ccggtagatg aatatcttct cttaactcaa
    18481 aatgtagatg aggggaatat tttccacctg catctccaat cgttccaatc ttttgtcctc
    18541 ttaaaaccat agcatttggt tttacaatta cagtttgaaa atgtgcatac aatgtttcat
    18601 acaatttacc atcaggcata caatgcacaa ttctaactac atttccccac ccaccaccaa
    18661 tatcttgcac aaaactaaca attccatttg aactagcata gactggctct cctaaatcca
    18721 taccatccac tcgattccaa tcttctccta aatgcgaatt tttaccgaaa ggttgagcat
    18781 cgtagtattt ctttgcgtta ggcgctccaa ctgggaaatc aaatccatct gatataaaaa
    18841 ctggattttc ttgaaaaatt ttttggtata tttcttcctt tgttcgttcc tttagaaaac
    18901 aaaaacaatg agtaaataaa acaaaacttg gaataaataa aaaaactttt actattttca
    18961 ttttaccatt tcctaaatcc agatgtgtca atatgaaatc gaagtccaga gtaaagccct
    19021 agaccaacct tgtattgcct accttctttt tcccatattt gaattagaat agtatgcaac
    19081 tgctctctag aaatcttttt cttgggtatc atatcaaatg cattaaaggt taaatggcgg
    19141 ctatttttag ctccaccggc taactgattg taaactcttg ttctataaga ggaaacaatt
    19201 tctacttcac caattttggg aataataaat ctattgataa aataaattgt aggtaaaata
    19261 tttttccaaa gatgttttgg aggaataaca aaaggtggct catccaagtt cttccattca
    19321 gtcccttgtc ttaaaatttc ttctatagta aaaacgttat taggcatttc tttcattata
    19381 tatttatgaa ccatatctat ttcattccag ttatccttgg agaaatgatt ccaatctgcc
    19441 cgagttggta tagatggctt tcctttatag cagaacaaga atattgacaa gatcacgatt
    19501 gcaatttggt atcgaatcat attaaaatta aaactcttta tcactttata taaatcaaaa
    19561 gtatagaata cgaaaccatt tacaaataaa atggagtaaa tagattatcg aatgaaattt
    19621 cacttactat tgcattctgc acaaagaata aaacaaatcg atttagtttt ttgattttac
    19681 aaattaagaa tcaatttcta agcttctaaa atactctaat ttaaaccatg atactcgaat
    19741 ggaagtcata tataaatcga tatacgttct atggttccct atttggtcta ttatttccat
    19801 tcttttcaac tattttacag tcttattcgt tttttcagtc gattgatatt gaactgctcc
    19861 ttttaacaca aaaaaacaca ccactgttgt ggatcatcga tacagctcct ttctttttag
    19921 gattactcgc cggttatgca ggtggaaaac aaatcactat aaaaaatgca ttggcagaac
    19981 tgatgcgaat caatcgggaa atgaaaattg aaatcattcg ctctaataca atggaaacaa
    20041 acttacagga tatgatttct acctataaac aggatttaca ttctgctaaa cttgttcagg
    20101 aattttctct tcctaaaatt cctaaaatga atgaatgcaa tatcagttat aaatacattc
    20161 ctctaaatcc agtaggagga gattttcttt ccattgtaaa atttcctgat gggaaactag
    20221 gtttattgat cggagatgtg gttggtcatg gaatctctgc tgcattaata gccgctctcg
    20281 cctatgcgct ttcgtataga gcacgcgaat actactcatt atcccctaaa agttatttag
    20341 agtatctaaa caatgaagca gaacaatatt taggggagga ctattatttc actgcactct
    20401 atggatttat tgaatttcag aataacaaag ctttcttaac attttcaaga ggtggtcatc
    20461 cctatccatt tatctataga aaagaaaagc ggataacaga aatttaccag atgccaggat
    20521 ctccgttagg tctcatgagg aatttatcct atgaacaatt aactattgaa ctaaattcag
    20581 gagataagat cttttggatt acggatggat tcatagaaat aagaaatgtt caggaaaaaa
    20641 ttttaggcaa tgatggactc gccgaaatca ttaaagagtc ctgtgaagaa aatcaagata
    20701 tacaccaaat catcgatgga attatttcca gatcagaagc atttgcaaat ggagctgcct
    20761 acaatgacga tagattaata cttggaattg atatattata aaatcaattc tttgttctac
    20821 gataggtcac tccgagtatc ccagaaggaa actgtttcga ttctacaact tctaagttca
    20881 ttttattttt tacagagtca aatagccgtg ttccgctacc agcaatcaca ggctgaattc
    20941 taagccaaat ttcatcaaag agagaatttt ctaaaaacaa atgccaggtt cgaattccac
    21001 tctccacaga aatattcttt ccctctttgg cttttaattc atttagtttt gatttcaaat
    21061 cttcaatgct agtatagagt aatttttcag aagcattcca atcgatatgc aaatcccgat
    21121 tgatcaaagc aaacttcttt ttagaattga tctgttttgc aaaatctttc tcaagaggat
    21181 cggaagaatt tagctctgca tttttccaat actctgccat cggattaaag gtatttcccc
    21241 caaataagac agtatctacc gtatcataac gctccattga gtcgtataac gtatcatctt
    21301 gaatagatgc ccatttttct aagtccgata caactccatc tagagacata tgcatgtata
    21361 aaataatttt tctcaaattt tttccttata ttacttagat ttagattttg ttttagattc
    21421 tgtttggggt aaagaaagaa cagaagttcc ttcaaaactg attcgttcgt cccttacaga
    21481 aactaactct ttcagttcat aaacattttt gtatccatac ccatacaaat taatataagt
    21541 tggaatattt agagcaagtg tgattggagt cgtattatta gctacaggag gtgctgcaat
    21601 tttagaggca aaatttactt ggtcatcctc aaaattatta ttacaataga tcagcacttt
    21661 cgtatcaaag cttggaatca ctctcgctaa atttctctgt gtaaaatcag aaaagtttag
    21721 atgcacagct ccttttatat gcttactctt atacatcgcg ggtgaacgtg tatctaaaat
    21781 tataactttg tcttctttcg acatttctaa aaatttttct agagaaacaa gacggttctt
    21841 tctatgctct ttcacctctg ccactatctt ttcaaatgca tcataatcaa catatgctga
    21901 tttcaaattc tccccatccg caaaaataga aatggataga aggctaaaaa ataaaaataa
    21961 tttcatacga ttactcctag tcaaactaaa ttgtttctta aagcaaccac tgtcaatgcg
    22021 aatactcact gtttgtttaa ttcttgactc gaatattata aaactgttac tgtattctca
    22081 tgatcgtata tgactttggt gcagaaaaaa aagagtccac ctatgctgat tatttgaaaa
    22141 ctccagaagg aggaaaattc caattaatag gaggagaaat tattgaaata acatcaccca
    22201 gtttatacca ccaagatatt ttattaaatc tcagcagcct ttttcgaaat ttcttactta
    22261 aaaataaaat cggaaaagtc tttgtatctc cattagatgt aatgctttcc gaaaaagatg
    22321 tttatcagcc agacattcga attctattaa acgaaagctt tcctaagatg aaagaaaaca
    22381 aaattgaagg tactcctgat ctagtggtag aaattctgtc tccttccacg gcatattacg
    22441 acttaaaaca aaaaaagacg acatacgaaa agtctggcgt gcgcgaatac tgggttgtag
    22501 accctatcca aaaaactgta gaactcttcg aaaatcaaaa tggaaaattt atctcctttg
    22561 ctgaacattc taaaaccgaa atcgccaaat cacgattagt cgctggaatg gaaattgaat
    22621 tagaacaagt gttttgaatt tagacacact tcttagatgg aacgcaatct tttttattca
    22681 aatatgagaa actatataaa actcttcttt caaattatct ttcttctttt ctttccgaat
    22741 tcagtcttta gtgatcccta ttattttatt aacgtccctt atggagtttt cactacaagt
    22801 tttaattcga ataacaatgg atccaaatct actgaaatga ctgaatcttt ttttatacct
    22861 agtttttcgc gtgttgaaat tcgagagacg aaaaaggacg aaacaaattt attcagttat
    22921 gattctgccc taaaaataag atataataat aaaattggtt ttctgcctaa atcgtatatt
    22981 ggaacgtata atgtaatcaa caaaaaagaa agtccagata gatcgaagta ttttgaaata
    23041 atttcccctt ataaaaattg tgactgcaat cggtatactg gctacatcga ttcctgtttt
    23101 atggagattc gttctaaaaa ctctaaattg attcaacgca taggaaacga taagcaaaaa
    23161 gcttgtcgga atcattactg gacaagagta aaagattggt tcaatgaaaa tgatctttta
    23221 atggatgggt atggagatgg agatgcagga agtggtggaa cctggagttt tgcaaaagaa
    23281 aaagtaaatt ggtctaccgg aaaaatcaat gaattatata ctgtaaatca attaaattgc
    23341 aaagactcag aggcaaagga tggttattcg attttgttcc aagtcgagag ttcaaaaaat
    23401 ttttatattc tctggaaaga aaaattatat cgagtagatg ctccgataat tcgatttggt
    23461 tctttcaaat ttaaattaat ggaagaaatg gatgcatatg attgtaaaat cgacttcaaa
    23521 aaaatgaaac caattcagga aagctcaatc accatttata aagaaggaaa gaattctatc
    23581 tcttataata accaaggaga gtttaatttc ttttacaatg gaaaagaaat ttctcaacat
    23641 taaaaaattt ctttcggaaa gtatcgatag atttgcacat tcaagagctt aagataacaa
    23701 agtaataagg aaacattcac atttgaaaat catcgaatct tttatcggtg tcctcggtgc
    23761 tcatctgtgg taatctttca aaatttttct taatactaaa gcattcatct aatagaagag
    23821 agtagatttc ttcgaatcgc cccgaatatg tcacaaattg caagtctcca tatccaaaat
    23881 aaagaataga ctgaatactt caattttgaa atatgtagat tcagtaatat acacggagtt
    23941 ctaattatga gcgaaactac gagtgcactt tccaaagaca tcgttggaaa aaaaatggat
    24001 cgttttgatt ttactgtgga acgtggcaag attaaagagt tctgcattgc aattggggaa
    24061 acaaacccaa tttatttcga tatagaagct gctaaaaaag ctggctatga tgatattcct
    24121 gctcccccta ccttccaaac tgctgttcag ttctggggct atcctaaaat ttgggaagat
    24181 atgacctctt ttggaattga tattaagcgt cttcttcacc taaaagaaga atacacttac
    24241 ctaaaacctc tctatccagg aaatgtatgg gcacaatcag aggttgtaga tgtgaaaaca
    24301 ggaaagatgg atatggtaac ttttagaact acctaccata attctaaaaa tgaaccatgc
    24361 attcaagcag agatggcgat tgtcatcaga cctaactaag gagtaattta tgaaaatcga
    24421 atttgataaa gtagaagtgg gtcaagaaat tccttctctt acactacctg caatcactca
    24481 tgcgaatcta gtgcgttatg cgggagcaag tggagatttt aatcctatcc acaatgacaa
    24541 agactttgca attgctaatg gtttagatgg aacaattgca catggtatgt ttgttatggc
    24601 actaatcggt cgcgcttgca caaattgggt agaccaaaaa caaattaaat tctttggagt
    24661 aaaattcaaa gcaatgacca agctaggatc tgttcttacc tgcaaaggtg taatcaaaaa
    24721 gaaaaaagaa gaaaatgggg aaaagttaat cactctcaca gtggaagctg tagatcaaaa
    24781 tggagaagta aaagccggtg gcgatttgat cgttgcttgt gcttaagagg ttaactgtgc
    24841 tataccgttt actcctgatt agcctcttct ttttttcatt tactctcagt gcagatgtaa
    24901 atgaagaatt catagaggca gtgaaagata caaacattga gaaggtgaaa atccttctca
    24961 aatctaaagt gaatctgaat gcgaccgatt caagagaaat gactccgctt cttatcgcat
    25021 ccgttgataa taatttagaa ttagtgaaac ttttagtaga agcaggagca gatgtaaacc
    25081 gtaaacaaaa agagacagga aaaactgctc ttatgtattc ctctgctaac ggtcacgtag
    25141 aaattctacg ttatcttctg agtagaccag gaattttaat caatgcaaaa gataaagaag
    25201 gaaagactgc tctcgtccat gcagttttct ttgcaagaaa agatgcagtg agtctactcc
    25261 ttgaaaacaa agcaaatcca aactctagaa caaattcaga agaatcagca ctcacatttg
    25321 caattaaagg aggaaggcct gagattgttt caatgctcag acaagccgga gccagagact
    25381 agaatgaagg atgcttttct caaatcaatt tctaccgtct atcgtttagc ttttttactt
    25441 tcgatcttaa tttttataaa tattttattt ggaccactcg tgcgtgcgac tgattctgga
    25501 cttgcatgtc ctgattggcc tctttgttat ggaaaagtga ttccgcctcc tgagtttaga
    25561 atctggatgg aagttggaca ccgtatctat tcaggtctac ttggttttgt aattcttggt
    25621 ctatccattg ttatttttaa gaattctgaa ctaagaaaac gatttggttt acttgcagta
    25681 accgctctag ttgtattagt caatcaagtg atactaggaa agcttactgt taccaaatta
    25741 ctcgatccag gaactgtaaa tatgcattta ctcaatgcag tgttactgtt cacattaatc
    25801 gtaagcatca cagccaaagc aaactacatt ctaaaatctg aaacagaatt agtctcattc
    25861 attcagtgga accagatttt tacgactaga aacttacttc tcacaattac tgtcattctt
    25921 atttttttcc aactttttat gggaggaaga gtaagttcta attatgcagg acttgcttgt
    25981 ccagactggc caacttgtca tggtagttgg tttccaaaaa tggaaggtct tgttcgtatt
    26041 caaatggagc atagatttgt ggcgtatatc attttaatct taattgtaat taacttttca
    26101 atttctattt ttaaatcata tgatcgacgc actcgtctct ttttaaaaat ggctcttcat
    26161 actgttggac tacaaatcat tttaggtgta tccaatgttc tatttcaact accgatccta
    26221 gttactgcat tgcatactgg agtaggagtt gcagtattca ttttaaatta cactgctttg
    26281 ttttatagaa ttttaccaga agataactct aattagccgc tcatgtcatc cattttaaaa
    26341 tacatttcta tctggaataa attaataaaa ccaagagtca ctacactcgt attggttaca
    26401 tccttacctg gtctctattt gggtggtgat ggtcatccaa ccggattttt agttgcaatg
    26461 actctaatag gaacattttt tatgtcctcc gcttctttta tcttaaatca atacattgaa
    26521 atcgatcgag attctagaat ggagagaaca aaaaatagac ctttgccaaa aggagaaatt
    26581 aaaccaaaaa cagctttgac tgtgggttta ttttttatct ttctttcctt tggaatttta
    26641 tattattttg ctaatctttt aactgctcta tgtgcgttag ctggtttatt actctatatc
    26701 tttttatata cgatctattt gaaaccgaga accgatcaaa atattgtaat cggtggaatt
    26761 tctggatgca ttggaccttt aatcgggtat gcagcggtta cgaacagttt gcctctctct
    26821 gcatggactc tttttggaat gatctttcta tggacaccag cacatttctg ggcgcttgcc
    26881 atttttctaa aagaagacta tgactcagca gaattcccga tgatgcctgt agttcgcgga
    26941 ttgaagaaga cagttcaggc aatcttcatg tatacaattc tatattccat ttcttgtgtt
    27001 ggattttatt ttgtatctga tcgagtagga accttttatt tgtattccac tgttgcattg
    27061 tgtttactca tgatttacct ctctattcga ttgatgattt ctgaatctaa aaaatttgct
    27121 cgatttttct tcttcttcag tatatttcat ttgtatttgg taaactttat catacttttt
    27181 gatagaaaga ttgtgtagag aaaatccgtt tatgaatcaa aactctctca taagcatctt
    27241 agtatcactg tgattttcta acaaagaaac tatgcagttt aaatcgtata atgaatttgt
    27301 atcttctctc ttatcctgtc agaaatgcaa aggaatgcag agcaagccaa tcgtaggctc
    27361 tgtaaaaaaa gctaaaatca tctcgattgg acaagctccc gggattcatg aagagaaaca
    27421 tggaaagcct ttttcttata cagcaggcaa aacactcttc aaatggtttg cttccattgg
    27481 aatccaagaa gagatttata ggcaacaagt caatatgtca gcggtctgtc gatgctttcc
    27541 aggaaaagca aagagtggtg atcgaaaacc aaatgcagaa gaaatagaga attgtagtat
    27601 ctatttaaag tatgaaattc aataccacaa accaagttta atcataccaa ttggtaaaat
    27661 ggcaattgat ttactaattg gaaattccaa atatactttg gaaaaagtga ttgggaacca
    27721 atacaaaact tccctttata aacatgagtt tgactggatt ccgctccctc atccttctgg
    27781 actcaatgtt tggaataata cccagatcgg gaaaaaatta attttaaaat ctctccaact
    27841 catccaatca catcccgcaa cgtataactt aatccaacct tccacatgaa tttcttcctt
    27901 tggtagaagg atagttagta aattttttct tgctatattt tatatattag ctacaaattg
    27961 aaagttatac ttatgacatt cgttacatca aaaccaaaat attccgccct atttcagtgt
    28021 atcaatccag attgtaagtg cgaatacgag ctccaagaga tagtatatct ctgtaggaaa
    28081 tgcggggatt tactccaagt aaaacacgac ctaaatgaat taaaaaagac ttctagcgaa
    28141 gaatggaaaa ctctttttga tgatcgctta aaagacgttc gatttccaaa ccaatcaggc
    28201 atttggagca aaagagaatg ggtattaccc gatctacgca ccgaaaatat tgtaagctca
    28261 ggagaaggct ttactcattt gtatgaagca aaaagactct ctaaagaatt agggctttct
    28321 gaattgtatg tcaaacaatg tggtgtttct cacacaggat cttttaaaga cttaggaatg
    28381 acagttcttg taagccatgt gaatcagctg attgcagatg gaattaaaat taaggcagtt
    28441 gcttgtgcga gcacaggaga cacatctgct gcacttgcct cctatgcggc aaaagctggt
    28501 atacctacaa tcgtatttct tcccgcaaat aaggtgtcta acgcacaatt aatacaacca
    28561 gtatccaatg gagcaaaagt tatttctcta gatacagact tcgatggatg tatgaaaata
    28621 gtgcaagagg tttccaaaga agcaggaatt tatcttgcta attcaatgaa ctcacttcga
    28681 atcgaaggac aaaaaactat ttctattgag atgacacaac aactcggttg ggacgttcca
    28741 gattggattg taattccagg cggaaatctt ggaaatgtat cagcccttgg aaatggtttc
    28801 gaaatgttgc aggaactagg cttgatcaat aaactaccta gaattattct agctcaggca
    28861 cataacgcga atccactcta tttatcatac ttaaaaggtt attcagagtt ctcacctgtt
    28921 gatgcaaaaa aaacgttagc atctgcgatc caaattggaa atccagtttc tgtaaaaaag
    28981 gctatccgca cacttcaaaa attcaacgga attgtagaag aagccgagga acatgaattg
    29041 gcagacgcag cagctcgtgg ggatcttcat ggactctaca atgatccaca cacaggagtt
    29101 gctcttgctg cactctttaa acaaatcgag aaaggaaatg ttcgaaaagg agaaagagtt
    29161 attgtaattt ccacggcaaa tggacttaaa ttcacagagt ttaaggttca atatcacgag
    29221 aatcaaattg caaaaataaa ttcaaaactc gtaaatcaaa ttataaattg caaagcgaac
    29281 atctcttctg taatggatgt ggttaacaaa ctgtaacctc aattatggat aacttgaacg
    29341 gaacaatccc taccaattcg gatgaacacc ttctaaccga aaaatttaaa tcgaaggttt
    29401 tacatggagt tgattctaat aaaacagtta tctttattac atatgtccta ggagagcttg
    29461 gagaagcaaa attaaaattc atcatcaaga caattctttt gaaatacaac cgaattgatt
    29521 tgatggatct tttatataca tgtgccaaag agcttattgt taattcaaca aaagcagcaa
    29581 ttaaaagact cacattcaaa gaaatgggaa ttgatcctag tgatgattct gagtatgata
    29641 agtttatgag ttcttttaag gataatttaa ctgataaaaa atttccacat tatagaagta
    29701 aaatgaaagc aaacggattt tttattaaga ttaagttatt ttataataaa gataaaatcg
    29761 taatgagaat tataaataat ttcccacttt tgaaaaagga agaagctcgc atcagagaga
    29821 aatttatgaa tgcaaagaaa tatgataatc tctttgagtt ttatatagag catggtgaca
    29881 atacggaagg agcaggaatg ggaatcacga tggtagaaat cctattagct caaagtggtt
    29941 tcgatcgtca tctatttaca attttctcat ctaaggttaa gcaggaaaca gtcgccaagg
    30001 tagaactccc tatgcatcca gattatgtac caatgcgata tggttttaat ggcaaagaat
    30061 gaactttttt atccgatata aaaatttaag ggcacaaaaa aatggcttcc gaaaacgaat
    30121 cacaaaatga attaatcaca atggcacttg agtcggtatc atccgatgaa aatgtaaatt
    30181 tagttttaga agggaagtta atggatgctt tgatagtaaa ggcatccgtc tttgtggaaa
    30241 ctagaagtgg tattctctta tttcttttct caatcgaaaa aggcgaatta attgaaatct
    30301 actctatttt tggaaactca cctcttatca atcgtattcg aacctttgaa aactattccc
    30361 aattcctgat tgatttaatt gaaacagtaa aaggaggagg aatcgaaaag gctctttcgg
    30421 atcggattgg acgcgcgctt tataaaggaa ttaatcctac taacctactt tctctttttc
    30481 ctaaaatggg aaagaaaaac tataatgatt tttgtatgat gatgcaagag atcatgaata
    30541 atgaattaaa aatacaaagc actaaaatgc aaatcgatat agagaaagtg aatagcttaa
    30601 aattcagaat caataatccg ttagctgaaa tctataaacc aattcaaaat ccatataaag
    30661 aagtaaaaaa agaaccaaca aaagttgttg ttccaatatt agaaaattct cctgaaaaaa
    30721 taaaagctga agcaatgatc aatgatgtgc aaaagaatta taaacaagtt cttatgtgca
    30781 aaacaattgt atctcctgtt gcgggtatcg attttgatag tctgaaagaa aatatgaaat
    30841 tgttatttgt tcttcccttt gaaactccag aagaaaaagc attggcaaaa agtgtaggag
    30901 ccgttagtaa agaaggaaca atcaaaccta tagctggaga atttcttaaa atcataacag
    30961 gtggtaaaaa tgaattccat atctttgcaa aaggaccaaa tggaatttta cttagagctt
    31021 tcgaagaaag acctgttaaa ttagctttgt ctaagaaaaa ccagcaggta gctaagaaag
    31081 aggagagatc tgaattaggt aaaatgatga gtgttgttgt aggaatttta tttgtattaa
    31141 tcatcatttt atcttttatg tatttagctt aaaacattta tgtataacga tagtaactat
    31201 aataaaaaat ttcttcaaat taaaccaatt ctgatattgt cttcatttat tgtgcttttc
    31261 ttttcatcct gcaaaagaaa tccgcaagaa ctaaaaatta aggaagcaat tcgaactggt
    31321 gattatatga ctgtagctgc tctttgctca gcctatcaat ttcccgatta caagactgaa
    31381 tgtgaatcca gcctagaaca agcggatcaa gaaattcaaa atataatttc acgaaaacaa
    31441 gaaatgcctt ttatgaaact cattctagat ccagacaaag gaataaaaat tcaggaatta
    31501 ttaaaagcaa atatacaaat gggaatcaaa tacagatcca tttggaatga aacagctgaa
    31561 atatacaaag aatagctatg gtaaaacaag aagatttaaa agcaatactt caaaaagcag
    31621 atttaaaaat cactcgaagt cgaattgaag tgttgaaaat actctacaat acacataaac
    31681 cgatcactca tacttatata atggagcacc tacccgataa agatgtgtgg gatagagtga
    31741 ctgtctatag aaccctttct gaatttgccg aaaaaaaaat tattaaatcc ttgccttcta
    31801 atgaaagagt cacatatttt gaaataaaaa atgaagccaa acccgaacat agtcatttag
    31861 tttgcgattc ctgcggtaag atggaatgtt ttgatgaaga tgcatacaag tttcaattta
    31921 aaaaaaatat tgaggatttt agggttcgat cattagaaat tatcattaga ggtctttgta
    31981 aaaattgcca gtaaaagttg aaaaagtcat aaagataaag aaatggactt ctaccgtttt
    32041 caattctaat gaaaaacgag tgaaagaatg cctgaaagag attctaccaa gatataaaac
    32101 aattcattgg gatgaagaaa agtttattca attttatata gatgtcctaa attcccttcc
    32161 ttgtagatac aaacaaaaga attcaattga acttactgga aaattaaaga aacaagttct
    32221 aatggatgct atctgcgata aattagacga actttctgct tcttaatgac acaggcaaag
    32281 cagctaatca atagtatccc cgatactttc aaaaaagacc tcgagttcat ttcaaatgaa
    32341 attcaaaaaa aaggaaaaga atcctttgta attggtggat ctgttcgaga tttaattcta
    32401 ggtaaatatc ctgatgaata tgatcttacc acttccatgc aaccagatga agttaaaaag
    32461 cttttcaaaa gagtgatcga aaccggaatt aagcatggca cagtaacagt tttaattgga
    32521 gatcgcagct atgagattac aacctataga cgagacatgg attatactga tgggcggcga
    32581 ccagatagaa tagaatttgg tgagtctctt tctgaagaca tgaaaagaag agactttact
    32641 atgaatgcaa ttgctttaga tctaataaat gaaagattaa tagatgaaca caatggattg
    32701 gaagatattc aaagaaaaat aattcgaacg ataggcgacc cattaaagag attcggagag
    32761 gatgggctta gaccaattag agcaattcgt tttttatcta ctctcggttt tacaattgag
    32821 gaagaaacct acttagcaat tttaaaaacg agaaacatta cagagaaaat ttctaaggaa
    32881 cgctttcatg atgagttaaa taaaattcta atttctaaaa atccatcaat aggcttaatc
    32941 gaattacaaa aaaacaaaat attcgaattg tttacaaagg caaaaataaa tcatcaaaat
    33001 tctgcggata ttgagatcat aggcaaatta gaattaaccc cgcttggttt aagactcgca
    33061 tttatgctag actggttatt agagacaaat gaaaaaaaag aaaatgcgga acaatttcta
    33121 agagatattc gatattctag agcgaatcta aatgattgcc tgttctatct tgaattattt
    33181 aaagaaaatc ccacgctaga aaatggatct gattgggaga atagacgctt tgccgctaaa
    33241 gtagtttctt atactggaaa tcgttccttc gattcttatt ttcatggaat tatgagcttt
    33301 ttaaaattat ttaatagcga gacctcagtt tttaagtttc aaaaagaaat tgaatccatc
    33361 cttcattcaa cagttccact ctcgataaaa gatttagcag taaatggaaa tcaaatcatc
    33421 gagagatttc caaacctaga taagcgcaaa atcggtctta ttctcaaaca tgcattagaa
    33481 aaaacaattg aattaccaga tttaaaccaa actgaaaaac tatttgattt aattgaaaca
    33541 tatctcatag aattgtaaat tactatgaag catatccgcc ctaagtttct agaacctcta
    33601 ttacctaaaa taaaaagtaa ttatcaagat gaattttaca ggcaaatgct tgtaaatgaa
    33661 aaaaagcgtg taactatcat cgcttcctta attgcattta ttctatttgt tcaaatcatt
    33721 ctaagatttt ttattcctga ttctctaatc aataaatatc caactgcatt agaaggatca
    33781 aaatattcca ttgcgatttt atttcttttt tttatttatg agatcgtagt ccggtttatt
    33841 ttctctaact ttataaaaaa agacattcaa ccaccagagt ttatacgtta tggaaatgca
    33901 tttgtggaaa caagtgtccc tacattggga atgattttgt ttggatattt ccaagaacca
    33961 acagatgcac tagtttcacc tattttatta gtttatttct tttttatact tctctctact
    34021 ttacgattaa attttaaatt atctttgttt acttcttttg ttgcggcagc tgagtatcta
    34081 ggtctttgtc tttattacta caacaccaat caaattaaat ctttaaatat gatgtcgatt
    34141 gaactcccac tcttttattt gagatcttgc ttttttttaa ttggtggagg tatatcggga
    34201 ttagtcgctg ttcaaatcag agaaggattt ttaaaatcga ttgaaacgat tgaagagaaa
    34261 aaacaactaa tgaatgtctt tggtcaacat gtatctcctg aagttgtaga taaattactc
    34321 aatcagaatg ttgacattga tagcgagata aaacatgtct gcgttatgtt tttagatata
    34381 agaaacttta caagtttttc agaaaataaa agtccagttg aagttgtaaa ctttttaaat
    34441 acgttattcg cttttatgat tgagatcgta aaccaaaaca atggaatcat aaataaattt
    34501 ttaggtgatg gctttatggc tgtatttgga gcacctatat ccaatgggag agattgctat
    34561 aacgcagtaa aagcttctag agaaatttta tcaatgacaa aagaatacat tcgtgttagc
    34621 aaaattcctc ctactaaaat aggaatcgga cttcattcag gaaatgcagt aacaggaaat
    34681 gttggatctg aacttagaaa ggagtataca gtcattggag atgttgtgaa tcttgcttct
    34741 agaatcgagc aattgacaaa acaatatgaa attgaatttt taatgactga agatgttttt
    34801 attcaagcaa tgcaaatccc taacgaaatg gcaaataacg agcttgattg gagggaaatt
    34861 gatacagtaa gagtcaaagg cagaattact cctgtaaaga tatttacctt agtttaaaaa
    34921 atatttgctc tctagataat attcttaaaa tgaagcagat gaaccaattc actctgaaat
    34981 tattttttat ttctttttta atattaatca cacaatgttc cctagacaac caaatgaact
    35041 atccgacagg agttccaaag gaagcaagct ttgatcgcaa aaaaaatgta tttgtcttaa
    35101 gaaaagataa gcatgaatac atttggtatg acaacggtaa actctattct gattgtgaat
    35161 taaatgagat cggtattcgc aatggtaaat gtgaattttt ctctgagttt aatggaaacc
    35221 tactcagcaa aggaaactat gtaaacaatt taagggacgg actatggcat tggtatttcc
    35281 cgaatgggaa tatttactac aaacaaaact tcagccacaa gtttaagaga gacttctggg
    35341 tagaaaccaa tctcatcgga aacgaacacg gaccctatga aagatactac agcaatggac
    35401 agctagaaga aaagggcgaa tacgaccaag gatttaaatc gggaaaatgg gaaaaatttt
    35461 atcctagcgg caaattagaa tataccggcg catacgagaa agacaataaa atcttatcct
    35521 ggaagttcta ttttccaaat ggaaacttgg aagcagaaga aaatttttct aataaggcag
    35581 aattcttaag tagaataacg tattttccga atggaaatgt aaattgtaaa aatgttttaa
    35641 atcaaccact tgattgtaag taacgctagt tcgatgtaac agaaaaatta aaagattacc
    35701 accgataaac accgaggaca ccgataaaag atatgataat tcaaaattat tcatcgttta
    35761 gttatcggtg tttacacatt cgacatgact cagtgcaagt cggtcataga tgttgtgaca
    35821 tttcgaaaat tatagaaaag aagttttaaa tcatttgaat ttgcaactga cgatagtaga
    35881 gattggtcgc agtgaagaag aattaagaag aaagatgtca ataatatttt ctttattttg
    35941 tattctcact tgtcgtattc gtccttattc tatcaagaat tttagttcgc gtttctaatt
    36001 ccttctctgt ggatttcgaa taatcaatac aaaaggtaaa atattttgct ccataatcac
    36061 ccttaaaatc aaatccgaat attccaaata aaaaatctaa aagttcatcg aaattaattc
    36121 caagtcgaaa tccataataa actccaaagg aaatttcaaa aatagtcaaa tcagaagcaa
    36181 acatataata gccgcttttc gttgtgcgat gtagcttgac gcctttatat ttaactatgg
    36241 ggctattatc tatggaaaac caaaatacgg gaacaacatg tagaaaatgt ttaccacacg
    36301 atctcgtatc ggacatatca tttattttat gatagcttgt acttgctgta aaattcgtat
    36361 cccctagtaa tatttcatac tctactctgt cattggagcc acttttaaga atgtgatttg
    36421 atcgaggtaa aatccgtatc ccctagtaat atttcatact ctacgggtaa atttttagcc
    36481 tcatcttcgt tattttgtat agaaatttta ttctctggat cacctataaa atattttcct
    36541 aaatatcctt ttcgtaatcc ataacctact ccgctattgg ctttttgata tccaattggg
    36601 acaaaaattt ttattctaac tccataaata ttcttttcta ccccaacatt tacaatatcc
    36661 aatgcgtcat tccctctatt tataaaataa gtttgcacac tagagcaaga tattgtaagt
    36721 aagcaggcaa gcattgtaaa tcgaattaat aagttcattt taattccttg tagtgtttta
    36781 ttttagtggc tatcgataat ctgcatccaa cttttttcat aatcgtctcc attctatttt
    36841 acaagtgaaa ggcttaaagc aaaaaagaat tgcgagtctt ttagaataga atgttttgaa
    36901 atagatgcgt atattttaac ttttttacaa actttataat tacgttttac tgttgacgaa
    36961 atcttaacaa gattttataa tcttagtatg aatgcactgt tggaaaatac aataaaagaa
    37021 ttaggttttc cgtctctgga agaatttgct aaacatcaag caatcgcgat tttaacacaa
    37081 aagattttga cttatcaaaa ggagatagag aaatatattt ctaaatacgg gatgaattat
    37141 tctgaatttt ctgaacgaca ttcagagatt agtcatatag acatgttgga aaaagaagac
    37201 gatcagatgg attgggaaat tgctcttcat agtcttcatt cattacaaaa aaaagtaaac
    37261 tcattaaaag aggatggcga catccctatg aatgaaaggg attatcgaaa acgaatagag
    37321 gattacattc tgaatgttcg atagaggata tattgaatcc ataattcaaa aatataatga
    37381 cattgttcaa aattatgaaa tcatcacaga cttgtttgag gataataaat acacactaga
    37441 attaagaata cttttcaatg ataattcaaa gcttgataat agagatgtat atttgtttat
    37501 agataatctt attaaaagaa aatataaatt tcaatggatg aaagctgaca atagtttaat
    37561 catgcgatgg gataatgctg agcatcatcg cactcttaaa aactttccat ttcatttaca
    37621 tcgatttaaa aaagaaaatg tagaagaatc gcccgaaatg actttagagt ctgttctatc
    37681 tttcataaaa aattttagcg gataacggca aatacttttc tctctagcac tatcgcctaa
    37741 tttgcatcca actttttcca aactgagtag ttacaaaaag atttctattt cctgaatcgt
    37801 atattctgtt ttatttacat aactcaaata ctttcgaatt tcttctggac ttgcattagg
    37861 atttgttttt aaacaagcat gaacccattc ctccaaagaa taatcataat aacttttctt
    37921 ggaataattc ttttttagtt tataaaattg ataacggcta atattatatt taccgcaaag
    37981 cccagaaatt tttcttctta ataaataaaa ttctttaaac agactttcta tttcttcttg
    38041 agaaatttct ttactcatat aaatcatact aagaatcaaa attgcaatcg gcaattaaaa
    38101 aacagtttta caattctcga tggaacaagg atactattgc aatatttggt tctatatagt
    38161 attatatcct tatgaacata gtgaggttaa taccatgaaa acgacaattc tactgctatc
    38221 atttatactc ttcttcggtt gttcatcaaa acaagatcca aattatctat ggcttgttgg
    38281 attagcacag agcactgcag ctgcaggcgt gggtagtcct ggtgcatcta ctcccaatgt
    38341 cccagcaaat ccttctgtcc catctcctgt tggaccacaa aattttacaa tgacagtcag
    38401 tgataataca ggaactccga cagattttct tttcgataca actaagactt taactcttga
    38461 tattgttgcc tatgactttg cagaacgtcc tgcaacaggt gcattggttc aaattggaga
    38521 cgcgaatgat ccagataatt ttactaccta ctttcaagcc gtcacaaatc caaacggaaa
    38581 tgtaactgga agttttactg ttaactcaac aacgagtcaa gtcaatctaa ttgttacata
    38641 caatggaatt caatacaaag cattaattga tatcaaaggt gtatatggga ttcgaagaag
    38701 tatgtatatg attggagtat ctcaagtagc aattattcag gatacagaca aagatggagt
    38761 tccggattcc gaagatattt atccaaatga ccctactcgg tcggctaaag tcaattatcc
    38821 gccaagcggc tattatacag tatcctttga agacttatat cccaatcaag gagatgctga
    38881 tttcaatgat tattcagtga gagtgcgtta tgaggaagat cttaacgcaa aaggagaagt
    38941 atcaagagtc cgtggatact ttcaacatgt cgcaaaaggt gcaggctaca atcatatact
    39001 tcgtttaagt ctaccttctg taaatgcgtc tagttatact ctcaagagac tatccaatgc
    39061 tgatattcaa gaatatcaaa ccagtggatc gaatgtggct tttcaagaaa ttgaaattct
    39121 tcctgcaagc aatacgactc tatctcaatc caatactgca agtggacaaa catatacact
    39181 tggtaaaaaa gctgagttag aagttatact ttcaaaccca gttccaaaaa caacgttagg
    39241 tgctgttccc tatgatttat acatttatgt aatcaataca aagtatgaaa ttcattttgc
    39301 agggaaattt aaaaaagcag atggatcaga tcaataccta gactctaatg gatttccttg
    39361 ggcagtatta attccaggaa attggaaatg gccttatgaa agagttgatg tcagaaaatc
    39421 ctattctgga ttccaacctt ggtatcaatc taaaggaact acaaatttag attggtataa
    39481 tttcccaaat ctctccgaag tattcccatc caattaattt aaaagtgagt tcgactgaaa
    39541 gcgaactcac atggatttaa ttatccagta aataacaaaa aaagaatacc aactactgcc
    39601 ttacacacag taatttagta gttgaagtag aacaattttg caagctccct gtatcaatac
    39661 ttgtattagt tgttaccccg ccgactcctc tgctagcaag acctgttgtg cttgcccaag
    39721 caacagtgca agcagagtta aatgtccaat cagttgcaag tccagtccac caaaaaatag
    39781 aaccacttgt atcaattact gcgagcaatg atcctgatcc aaaatttaca ataccagctg
    39841 aattcgtagt taatatcttt gtatcatcag ttcgatagta atctgtgttt gcctttagaa
    39901 cccaatttac attttcggat actccaccag agcaattggc agttgaacaa gcaattcttg
    39961 ttgtcccatc aactaaaaag gctttatatt cagatgcgat acccgctaga ctactaaagc
    40021 tcgcattttt ttgagactga caataactgt ctgctcctgt tattccgcca aggtttgcat
    40081 tggtatttgc tgtaatgaaa aaatattttt tatcatttgt agttggtgtc acaggtgtag
    40141 gtgttccatt tgaagttggg actggtgtaa caggaattaa tttccataaa atcactacgt
    40201 caaatagttt accctttgga aaatcttgtg agcaagaaaa gaaaaatata cctagaaaaa
    40261 tagtcgttag tatgaatctc aatcgattta agaattgact cttccttttt aaaaatttaa
    40321 acctatcaga gttttctagt atgattcgga ttctattttg cataaattcc tcttatctag
    40381 taactcatac taaatttcat ttcaaaagaa tacatagaat tttcagcttg atataccctt
    40441 tgtccaaaag actcagatct ctggtttaat tctatagtaa cattagaagt agaaattttt
    40501 cgagcttgcc aaacctgcaa tgcataactt aatattgcaa caactccgac tgccacttga
    40561 tttgtttgat gtctattgta ttcagactct gcatcatttc ttctctctct caaatagata
    40621 tctccaagta acggagtttt taaaccaatc gaaattgctg ataaataaat cggatcattc
    40681 attggatcac tcgcaatgcg attgccagct agcctttcca tatttccata caaagcaaga
    40741 ccagtgaacc aaagaatcca gagactacct ttgatttttt gattgttttc caattgtgtc
    40801 catccaggaa taaaccactt ccatttctga aaatttcttt ttggctctac ctcaactact
    40861 tcctgagcaa cttgcttttc gtcttttttt agaggtttcg gtttatcttt ctcaacatag
    40921 aattcctccc acttggtcca agaagaaggc tttccaaatt tattaattga tgcaattcga
    40981 tattcataat agccggaagg aactttgagt tctatattat tttggaccag tttctcttct
    41041 ctttccgtat ttgctgccct gtctttaatt tgaatcatat aacccttagc accaggaaca
    41101 ttttgccatt caatataata actaaaaatt ctcctttctt cagagtgaag actactaacg
    41161 ataaaagaca gtataaataa aactaagaac ctactcttaa ttattaaatg taaaaatttt
    41221 agaggtttgg gaaattgttt gaaatcgaaa agataaggtt tcatactctg aatcaactta
    41281 gtaaaaaatt ggaattcttg caactcaaac aaataaaatt caaagatacc attctaactt
    41341 aaatttagat ataaactcgg aatcatttgg taaatagttt ttttcttgac taaaggctaa
    41401 gcaaattgtt ttaagtatat acaatatgtc tagcaaaacc cttaatccat ttctaacaat
    41461 ctcagaaatc acaatggcga tgaattctac tttggatcca gatcaagttc tggaaatgat
    41521 tttagaacac tctctaaaat tctccaatgc agaatcaggc tctttgatgt tagtaaatag
    41581 tagagagtct gttctagata tcgtaacata tagaggtcat gaccctgaaa tcaaaaaaga
    41641 atttcgatta aagataggtc agggtataac aggattagtc gcatcttccg gaagaggaat
    41701 gattgtgaat gatgtaagta aagatccaaa ttacatttca atcaagaaag aaatccaatc
    41761 agaattagca gtgcccttaa ttgtagaaga tatggtaatc ggagttgtct ccctagattc
    41821 aaatcgaatc aatgcattca ccgaagaaca tttaaaaata gtaggaattt tagcaaacca
    41881 ggctgcatta atttttaaaa acttacaaac attccgaagg ctagagcaaa aaaataaaat
    41941 ccaacaagta ttaatagaga tatccaaaat cgtaacatcg agtctagacc tagatgaaat
    42001 ttttcaatcc attatggcga ctatcgaacg atcccttaaa ttagaaaagg gtagcctagt
    42061 attatatgac aatgcctcag aaacattacg acttgttgcg gcagttggtc tctctaccga
    42121 tgaaatcgaa aaaggggttt atcaactagg agaaggcgtt gttggaaaag tctatgaatc
    42181 gggagaagca atgctgattc gttcagtagc aaatgaccct ttattcttaa atagagttgg
    42241 atatctaaat cattttaaaa atgatccaca taacgttagt ttgctagctg caccagtaaa
    42301 aagtgagcag tccatcatag gagttctcag tgtatttttg gttcatcaaa aaaatatgga
    42361 tacacaaacc tatttggatt tcttgcaagt cgttgcatcc attatttcgc aagctttaaa
    42421 gattcgtcaa cttgtggatg atgttaaaaa agaaatctca agagaaaata ttttactcaa
    42481 acgagagctc aaagaaaaat ataaattcgg atcgatcatc ggtcgctcaa aaaatatgga
    42541 aaagctcttt gagaagattc agctcgtatc agactcaaga gcatccgttt taatcacagg
    42601 tgaatcagga actggaaagg aaatgattgc ctctgctatt cactataata gcaatagatc
    42661 tgaaaagcca tttataaaaa tcaactgtgc tgctatccca gaaaatctat tagaaagtga
    42721 attatttggt catacaaaag gatctttcac tggagcagtt tccgataaaa aaggaaagtt
    42781 tgaaattgca gatgggggaa ctatttttct agatgaaatt ggagaaatgg atttaaactt
    42841 acaatcgaag ctacttagag ttttacagga aaaagaaatt gaagctgttg gctctcttaa
    42901 agttaaaaaa gtagatataa gaatactagc ggcaacaaat gcaaatttag aaacgttgat
    42961 tacagagaaa aaatttagat ctgatttata ctatcgttta aatgttgtaa atattcatac
    43021 tccaccactg agagaaagaa cggaagacat tccactcctt acaatccatt ttattgaaaa
    43081 atactctaaa gaaaataata aaaacatcaa aggattcaca cgcgaagttc aaaagctact
    43141 cgctctttat ccttggcctg ggaatgttag agaattagaa aatgtgattg aaagggcagt
    43201 tgtaatgact cagaattcaa tgctcgatga atctgacttt gtggaaatta cagaaaaact
    43261 tcaatccatg aatcttccta tcgcaaaggt ttcagaagag acaagcagtg atttatcagc
    43321 gggactttat agcatgccca atttagatgc aatagacggt agagctttag aagttgttgt
    43381 cggtgaggta gaagcaagac tcattcaata tgcactgaaa aaatttagat ataccaagac
    43441 aagggttgct aaatttctag gtatcaatcg caacacttta gataagcgta tcaaagagct
    43501 aaagatagaa tattaattca gtttccattt ttttgaatat ttgaatttgt atttttaagt
    43561 agctatattc ttatcttaaa agagcaagct gtatcataaa aataaatcta agattcaaca
    43621 tcagcatgga ttgctttttt atttgtttat tttatcagaa aaattgctga gcatcaatca
    43681 aagtataatc aagaggttac caagtatgaa gaaaaatatt tttttaatca tgttatttac
    43741 tatcagttgt ggaagtggtg aagttaaaaa accaaattta caacaagaga caatgagaga
    43801 gttattgaga gaggattttt tattaaagaa ttatgcaaca atagaaatga atcgcggtaa
    43861 aaatataact gcagttctta aatttcaaat tcagaaaatg aaagaattag aagatagaca
    43921 caaaggaaat attccagcag aatttcgaga taaatacgca cttcataaag atgaatacga
    43981 aatagcactg catgatattg agacaaaaga aattgatatg gttgctaaag gagtaaaatg
    44041 gatcatctct gcatttggct caccaactgc atttgtattt tcaacaataa agactgcaat
    44101 ggagttaaat aaaaatttaa atgatcaact aaagcatcca agaatcaacc aaacatatga
    44161 atctttaaag cgatatgcgg aagcctctaa tgttaaacta gtagatgatg aacaagttag
    44221 taatcttcta ccaaatgcct ataccataaa aggagaaatt gattctaaga aggagtggca
    44281 aacactttat ggacttgtaa ctgcaaaatc taatctaatt atagaaacaa caggacagtg
    44341 gtctattacg aataacggta atcaactcaa tgcaaatgga acagataaga gtccttctga
    44401 ttggtttgat tatagagtcg ataaaaaatt taattatggt gccttgattt gcagaatcgt
    44461 ccaaacagag aaagaaaaag atttcccaat ttttagttta ggcaactcta cagcagataa
    44521 agatggagta ttggaatgtc gtattaatga tacagataca ggtaataaca caggaagtct
    44581 tgaagtatcc atcataataa aaaaatccta agtttccatt ttacaaaaag aaacaaagaa
    44641 tcgataatag aagggtaatg actttattta aaaaagcaat tgtgactgca ggaccaacta
    44701 gagaatggat tgatcctgtg cgatatatct caaatgcatc ctcaggtaaa atgggctata
    44761 atatagcaca ggaaatcctt tcttggattc cgaatacagt atacgtgcat ggaaatgtct
    44821 cagaaaaata tgctaaggtt actggtgcaa aaaatcttta tgcagaaact accattcaaa
    44881 tgagagatac cttattgcaa gaattgacag aagacacact tgttatcatg gcagctgcac
    44941 cagctgactt cagacccaaa tccattgcag aaaataaaat caaaaaagag tccgaaggta
    45001 acctcaaaat cgaattagaa aaaaatcctg acatactagt ttccttaaac tccttgattc
    45061 agaataaaaa tataaaaggc agtgtattag ttgggtttgc tgcagagaca gaaaagctag
    45121 aagagcatgc tttaggaaaa ttagaaagaa aagggttgaa atacattatt ggaaattatg
    45181 taggaacaaa ttcaggattt ggtgaagtag aatcttccgt aagaattttt gggaaatctg
    45241 gtttgttaaa agaaatccaa actcaatcca aagaattaat cgctaaggaa ataagtcttt
    45301 ttctaagaga taacgcataa aaactatgaa agtcttttca atggagaata catttaagcg
    45361 ttattgggac ttattcatat ttctactcgc aacctattgt tcaatcgata ttcctttttg
    45421 gattttattt ccatatccgc aaacaggttt ctttttcttt tttgaattat ttatctcggt
    45481 agcatttctt ttagatctta ttttcaattt tagaacctcc tactataaaa atgaaacgtt
    45541 aatcaccgac ccttggggaa tcgcaaagaa ttactttttt agctggttca tattagattt
    45601 actttcagta attccactcg aactcatgat tgagcatgga tttatgccaa agtctctttt
    45661 attcttatgt atattccgat ttttaagaat cataaagcta accgatggag tgcgattcaa
    45721 aagaacttgg ggcatgcatg aattcgacag cactagtttt atgcgtttgg catttctaat
    45781 ctactggata ggaatcttcg ctcattggac agcttgcggc tggatcgtaa tcgatggagg
    45841 agcaggagaa gttagcaatc cagataccgt aactagatat attcgagcca tatattggtc
    45901 agtaacaact ctaacaacta tcggttatgg agatataact ccaaaaaaca atgttcaaac
    45961 tatttatact atgtgtatta tgcttgtggg agctggtgcc tatggttatc tcgttgcgaa
    46021 tatcgcatct attttagcaa atacagatat actcaggact caatttattg ataaaatcca
    46081 gaagatcaat acatttatgc ggtataaaaa aattccgaat gaaatgcaga aagctatttt
    46141 agattattat gattatatct ggaaaaatcg aaagggctat gacgaaaatc aaattctaaa
    46201 agagcttcca ccttccctta gaatgaaggt aagtatttat ttaaatagtg atttagtaag
    46261 aaaagttccc atactaaaga atgcaagtga cgagctgatt tcaaagataa tagtaaacct
    46321 tgtccctgta gtttatatgc agggcgattt tatttttagg aaaggtgata cagcccatca
    46381 tctttatttt atcagtaaag gacaagttga aatcattgga gaagatggaa ttacaagcat
    46441 tggaattttg agtgaaggaa gtttctttgg agaaattgca ttgctcttag atactccaag
    46501 aacagctagc attcgcgcta ttgactattg cgatctatat tatctagaca aggaaacttt
    46561 taaaacaatc attaaacaat atcctgattt tgccgcctat gttgataagc taattaaaga
    46621 tagacaacaa tctagggcag aattgaaaaa tgaataactt gtaaacatcc ttctccagta
    46681 gaaaatttta catccttata aatcttccta tccataaagt ttctagctgg aaaaggggga
    46741 ccaatcataa atgaatgatt cttagattct acagagtatt gaatagaaag ctttggatcc
    46801 tcaatttttt ttaattgtat ggagttgtga agttttttaa accaagcttc cggcaagtaa
    46861 tccttttcgc aagaatctga tataagataa acagcgctgt atccttcctc tcttgggtgt
    46921 tttttaaata tcttaggatc gatactccat tttaatttat tgagatagga aagtttagaa
    46981 agcgttaact ctgtatctgc tttatttaca tggtctggat ttataaagtt aaagaaaatg
    47041 gcattctcct taacgtcttt gaaaagtttt tctacttctc taggagtaaa aaaatcatct
    47101 ttatttttcc ctgtatagca aatattttcc cccgaaaatt ttgaagcaaa cttctgacca
    47161 aatgggaaaa ttaaacctat agataaaaat aagaccaata tataaaatac caatttatat
    47221 ttctcttgga gactagagag caaaagcata aaagcaatgg agatagtagg taatagatga
    47281 taaatatgcc ttgcttggtg atttggtgta aaaaaagtta aaattaaaat tgtaagaatt
    47341 ggaaataatg aaaagagaaa atagagttca attttcttat ttttataata ttgaatatat
    47401 ccaataacaa ctcctacaat caaacctatt aaaaataaaa tacccaccca ttcaggaata
    47461 aatgcctgac ttgatagtgt tttaaaaaaa actaaataat aatctaatcc tttttcaact
    47521 acttccccaa cagcatgtcc ttctgattga atatgaccta aagtgctact agaactagaa
    47581 aaccgatctg gatgaatcaa aacaaaaacc acaatcggaa ggaataccca ttgaaaaata
    47641 ccaatcagtc gattgaattt taaatttaac aactgactca tttgatgatt caagtataaa
    47701 taaaaatcga aagaaagcaa aaggataatc aagtatttaa tatagttctt tgtttttcct
    47761 tttaataaag attcaggaaa tagaatgtat gaaacaatta ggaggataac aagtagtagc
    47821 cgatattgct tctttaaatt atgagagata aaggctatat atctagtaaa aaagagaact
    47881 gtttcatcta gataaagact tacatgcaat acaagcatcg cgacgataaa caggtatcca
    47941 tatggatact tggtttggaa taatgcaaaa gttgataaag ataattttat taaaatagat
    48001 ttatttcgga taaactcgtc atctcgataa aaaagatgca aataataaac gctcgctaaa
    48061 aaaaacacag ctccttgaac ttctaacatt gcagaaaaag tataaattaa aattggcgga
    48121 gaaagaaaaa cagaaagcca gactggtaaa aataataaaa cacttatcca ttttttatct
    48181 aagagataaa atagaacatg aatcgccaaa cctaatagaa tagcaaatgt tacgagtgta
    48241 atcacagtat cacttgtagt agataccccg ctagcaaaaa aaacaacaat ttgtattagg
    48301 tttctaagta taggccaagt tggagaatcg agtaattgga aagcaaatga tagaaattca
    48361 aaatgacgaa ttgaatccat tagctttaaa gtttttatga accgaagatc cgcatcccaa
    48421 gataagaagt ctttatttgg acaaatagta ttaaattctg aaaataaaaa atataggctt
    48481 aatattagaa agggaatttg aatcaaacac aataatataa aggaaactag attttttcgg
    48541 ctcataatgc aatcaatatc cagatactta tcttgaaaat ccaattaaaa ttttctaact
    48601 tactaatttt aattgaaaaa gttttttcta taaattaaac tgttaagaaa catgtcagaa
    48661 atccagaaag tagaaagtcg atccgtaggg atgtttgtaa ttcctgaaat tacggcaaga
    48721 cgtttaaata aaatcctccg aacctttgtt gaccatgaac aaggaaagga tatagaggct
    48781 attctagaaa gcatcgtaga tccggccact ttatctagaa tcatgaggga caatcatttt
    48841 tggatttcaa gtgctaacga acaaaagatt ctctccaaaa tacagcagaa aatcccttta
    48901 gaagaaatat tttttaatat ggggaaagag ttttttctag cagattgttt cgaaatctta
    48961 cctacggacg atgttcaact agaattcgaa gagcttattt ctagaatacc aattcttata
    49021 aaccaatata ttcgttatat cgaaattaaa acaattagct actcaaaaaa caaactcagt
    49081 atagaattta aatttgataa gaagataaaa gaaaatgctt atgatctaat tttcttaaga
    49141 gggatcctcg agggtgcagc aattcttttc caagttgttg gatgcaaaac aacgttactt
    49201 gaaacatccc tagaaaaaat ggatgagcga ttttttgaaa cacattcaaa tacaaaattt
    49261 aattcaaaaa aatgcgttgt gcaaatcacc tggattgatt gtaaaattaa agtagggaaa
    49321 acaaatataa ataaaatttc cccagccttt gagactaaga catttgtaat ttccactctc
    49381 aatgattcaa atcaagggga gtattcctat attgatttaa acgatatttt tagcaaatcc
    49441 aaagaattat atatcgagaa tcgagatttg gaagctgctg ttgaggttct aaaaagttta
    49501 agaaatgaat taatgataaa gcaaaaatcc ataacaaagg atttaaaaat agcaagaaac
    49561 atacaaaggg gaattatccc acaaagaatt ccagactggc gagggttaca atttgcagtt
    49621 agttttatgc caatgcaaga agtaagtgga gattattatg attattttaa tttcggttca
    49681 aatcgtttag gaattttagt aagtgatgtt tcgggtcacg gagttcctgc tgcctttatc
    49741 actgctattt ccaaattact attcacaaat tataaattag atagtccttc tgaaatatta
    49801 ggaaatgcaa atagtgaact cttagatctt gtaaaacaac aaggatacct tacatgtttt
    49861 tatggaatat ttgattctaa ttatgaaatg acctactctg ttgcaggcca cccacgccct
    49921 atattactca gaagcaaaac aaaagaagtt gtagtattag agggagaggg aacctttctc
    49981 ggtatgtttg atgatgcaag agatcatttt agagactata aagttaaact agaaccggga
    50041 gataaattat ttgtttttac agatggatta atcgaagcag taaatgatgc aggagaacag
    50101 tttcaacaag aaaaattgat tcgattcatc ttagactctg ctgatatgga tgttaagtca
    50161 tccatcgatt atataatcaa aaacttcaat caatactgcc gcggaacaga ccaaggggat
    50221 gatattacgt tactcggtat tggtcttagc ttaaatatgg aagcatttga taagcaccac
    50281 ttagaagcgg agaggaattt taataataaa aattacgaac tcgcagcaga acatttaatt
    50341 aaagccaata gcattctgcc aaatgattta agtattcttt tgttacttgg caaatgctat
    50401 gcaaaaacca gaaattatgt ggaagcaatc aaatacttag aagaatacaa tagccttaaa
    50461 acagataacg ccgattcaca tttaatttta ggatattgct tttataaact ggaaaactat
    50521 ggaagggcag aactagaatt acaaaaatcg atttcactta gaagtggaaa tttaccagca
    50581 ctttacaatc tagctaaaac ctttgcgaaa gaaaactatc cggataaggc tttagataca
    50641 ataaataaaa ttctggtaat tgatccttat tatacaccag cacttaaatt aaaaaacttt
    50701 ctaatgaaga aattaagcta atatatacgt tagcctatag aatgcagaaa gtcctttatt
    50761 tcatttaaaa ccatctctct atcttttgga agttcattca tcaattcatg ataaagccct
    50821 ggataagtct tgataatttt cttttcagtg gttaaatacc taaataaatc cctactccca
    50881 ttaacatcag caattccatc ttgctcacca tgcactatca aaaccggtat atgcaaatga
    50941 ctcgcttttt cgtataacgc tttagagaga tgaaataggt ttgttgccat ctggaaagaa
    51001 atttttccgt gagttaatct atcattttta tatgcttcaa ttacagattt atcatgagaa
    51061 aggtaattta aatctagatt cgcgtcaatt gtcatatcag gcatgaagga agccaataat
    51121 tctgccatga ctttttttac ttccttttca aagtccatct ttggtcgtaa tccaggggaa
    51181 cttaagatca ttccatctaa attatcttga ttaaaacctt ctaaggaata ttgcagagca
    51241 atcacacccc cgagagaatg cccaagtaat gtaaccttat ttaacttttg ctcctggcga
    51301 gcgatataaa ttaattcggt taaatcttca atgtaatact gaaactgagc aacgtgtcct
    51361 cttttgccat cagagcgacc atgtccacgg gcatctaaag cataaatatt aaaattatca
    51421 tcttttaatt tttcaattat atttccatat cgatcgctgt gctccccaaa accatgttgc
    51481 aagattaaaa ctctgtttgc tttcggcttt gtccaagatt gataaaaaat ctttgatttg
    51541 tctatggagg attgaaagtt gctttgttta ttcgtaaatg ccatttttcc cttcttcgtg
    51601 cgaattatat tcttagaaag atttgattcc tattctctaa tctgttgtaa aatgaacaac
    51661 attcacgtca agatttattc atttataaga agcgagaggc aaagtatctt aaatcgtttt
    51721 tttaaaagca atttataaaa atatttcgaa ttcttctgtt ttattgattt acctatcggt
    51781 atataaataa tgtaaatcag ttccattgcg aatcggctta agtataaatc ttggtattta
    51841 tgagatagat tttaagagaa gaagaagaaa gtattttacc tgttttattt tcattattcc
    51901 tactttcatc attcattcag aaaataaatt cgtctaggtg aattatgcat tcaaatcagg
    51961 tcttttcgaa atatccattc aatcaggaat gaaagtgcaa agagtcttcc aagtattgct
    52021 tgtctttttt cctacgagtc tatctttctc acaaactatt gattttatta gttcattaga
    52081 actttctaat attctcattt attttttaaa cacataacaa gagaaaaagt tacggataga
    52141 tatacttttt atttctcttt tagaattagt ttgacattat aaagttattt tttttaaaat
    52201 taaatcatct atgtcaaagc ttacatcagt atttatttca attcttttta tactccttct
    52261 agattcttgt tcatcacttt ttcaaaaatc ttggaaacct acggataaag aactactaaa
    52321 aacaatccgt gtaaatctag gaaaatttga ctcttcacaa gaaataaaat caaaaggcaa
    52381 gattcattta caaactcaaa gcgatagtcg tgatattgat tatattttct cgtttcaacc
    52441 ttccgatgga aaagaaaaat ataaattaac atttgataca aatcaattta gcctaagaga
    52501 tattccatac agaggcgagt tagaaataat tccagaagtg aacagtggga agtttatttt
    52561 cgtaaacaaa gtcccgatgg aagattattt aatgggagtg gttccggcgg aaatgccatc
    52621 tagctggcca ctcgaggcat taaaagccca agcagtagca gctaggacat atgcagtaga
    52681 ctcaatactc gcagctccta atagcaccta tcatgttgaa tcaacaacac aatcccaagt
    52741 ctatggtgga ataaaaagag agactgttcg ttcgaatcaa gctgtaaggg agacagtagg
    52801 cgaaatacta atttttcaaa ataaacctgc aagaactttt taccacagca atagcggagg
    52861 caagactgaa tctcctgaat ttgtttgggg ttatactggc ttaaattatc ttgttgctat
    52921 tgactctcca cattgcaagc tagcggataa ctttcagtgg aaaataacac ttacccgcaa
    52981 ccaaatgcag gaaaaactaa aatcactgga attggaggaa attaagaata ttcgaatttc
    53041 agaatatact ccttcgggta gagcaaaaac tctagaagtt gaaggaaagg aaaaagtagt
    53101 aacaatgaat gctgttgact ttagaagagc tatcggacag accgttatgc gatctttact
    53161 atttgatatt gatttagaag aagatacttt caaaatcaaa ggtcagggat ttggtcatgg
    53221 tgttggtatg agtcaatggg gaagtaaagg tatggcagaa aacaactatg attataagga
    53281 aattttaagc ttttattaca aaggaaccga tatggttaca atcgattgat gatttcagtt
    53341 caaaaagcaa ttgaattgat tcttgataac actcatacga ccaatagaac ccaaaagctt
    53401 ccaatttatt acgcaaagaa ttttatactt gctcaagaga ttttctctga tagggattat
    53461 cctcccttta atagagcgac tatggatgga tatgcaatcc gcttcaatga cttttcgata
    53521 gatcgacaat ggaatgttat aggagaaatc tatgctggag ataattggga ttatccaaac
    53581 ctaaatcgag aattgcccgc tattaaaata atgactggag ccgctgttcc agaagatttt
    53641 gatgttgtta ttaagattga agatacgaat agagttggaa ataaaattca tatcctttca
    53701 gaaaaagtaa aacaatggat gaatatagca aagaagggag aagatactca atctggaaat
    53761 acaattgttt cacaaggaac ccttatagac gattccattc tcaccatcgc agggagtatg
    53821 ggacaattag aactaaatgt atttgaaccc ccaactgttt cgattttttc tacaggaaat
    53881 gaaatagttc cgatgggtca aataccaaat tcaaaccaaa taagagattc gaacacgata
    53941 ttaattgaat ccatgtttca aaactttaaa tcaaaaataa aaagaaaggt tttattgcca
    54001 gatgacttgg aaattataaa gaaccatctt gatcaatcga gtgattctga cttctgtgta
    54061 attacaggag gtgtatcagc cggggataag gattatattc caaaagcact agaatcgata
    54121 ggcgcgaaag agattttcca taaagcaatg attcgtcctg gtaaacctat atggtttgga
    54181 aaaaaagata atactctatt ctttggatta cccggaaatc cgttttctgc tcgagttaca
    54241 tttaaagtat tcatagaacc tgcactttat aaatttatgc atttaaataa accaaagagt
    54301 atttttcttc cactcctttc agaaagaaaa aagaaacatg gaatggaaga atactttaga
    54361 gcaagaatcg taaataaaga aaataaatcc tatattaaag aaatatcctc aaatggaagt
    54421 ggtgactatc taaatagttt tgggacagat gggattgcca tccatcccgc aaataatatg
    54481 gaattacctc aaggcatcaa tcttgaattc ttatattggt aattaaaaac gataggagaa
    54541 accaaactga aaacttcctc tgccttggtt tcccatatta aaaaataatc ctgatgtctt
    54601 ctcaggtgca tttatgattt tatctgaaga tttacggtta atcaaaatat cgacaacgtt
    54661 atacaaatag atagcggttg caactcccac gacagcctct tgtttattaa cttcttcctg
    54721 agcattattc ctagattcaa tagattggct tcttaaataa attgcagcag gcaataaatt
    54781 tctatcacct ggtaaaagaa atagcaggtt agaaaattga tcagatctag acaatgagta
    54841 ttgtttataa ctctcaaaaa agaatacact actcagaaaa aataccacag aacctccaaa
    54901 gatatatgct ttggtttttt cgcctttgta atattgtccc cagccaggaa tcaaactaga
    54961 tcgccagaga ggggggaaaa cttcagcaaa ataggatttt ttaggttcac ctttggtcac
    55021 ttctgtagtt gtatcagttc cttctctcgt ttcctttgct actgaattgt ctttgtctat
    55081 ctttgtcgga tcatcaggct tagtgtcttt ggtctccgct atattaggct ccgctgattt
    55141 tggtttttcc tgcaaaagaa caaaggaatc gactgaagta gatttattta aaggatttcg
    55201 taacgttaaa cgaaaagaat caggactagt gaaagtttcc aaattaacac taaattttat
    55261 ttcagactct gaaactcttt ttatctcatt aataggaact atcccattgc ttgattccaa
    55321 ttcaacttcg atatcttcca gaaaatactt accttttact actatatctt gctttttctc
    55381 gggaatggta aaagtatatt ttaaattttc ctctacaatt ggaggctgag aataaatcac
    55441 ttctaggcta gaccatttac tccaatgctt tactttcttg aatttgttca aagcaccaac
    55501 tctatattca tactttcctg gtttcaaatc tagaaatact tcattttctg tggaattgat
    55561 ttgcatttct ttctctttat ctggtcgaat ttctaattta tatccaattg cattttcaac
    55621 attcttccat ttaattgaag tttttttcgg attattttca gtggattgag caataatact
    55681 atttgccgct attaaaataa aaagtaataa atattttatt cgcattcgat ccatccttaa
    55741 ttaaaatttc ttatcaatat gttgttttcg aggagaaata atttccggag ctgtagaagg
    55801 tgaggttttc aaagaaattg aaaaataatc cattgcaatt ggagaaatat attctttacc
    55861 ttctgaactt ttcacctttg cttttacttg ccacttgaat ttaccttcat ctaatatatt
    55921 taaatcttta attgtgtatt gattagcaat cgtatcatca gatataagaa gtttgtctcc
    55981 attttcaact ttttgaaaca tctcaaattt atatgatatc gcattcggaa ccgtatccca
    56041 tttaaattgc aaaacatctg aatctgacat atccacagtc tctgctttta caggatagag
    56101 tagaactggc tcagaaatgg ataaaggaat tcgaaaagat ttctgcttac tcctaagaag
    56161 ttcttcattt gtttcttggt taataagtgt tagacgcaca tagtaagttc cagatgtaaa
    56221 tccatcaagg cttacaccaa aggaagtagt catttctgat ttatagattg ttttaaaatc
    56281 tggtttatct gatatctcta atttatatgt tccgtagata tcgatcttat tccatttaaa
    56341 agcaattttt tcatttggtt ttatagcaaa ctcagcgtta tccgctggag ataacaattc
    56401 taaactagca ggttctctaa cttcaaagcg ggagctagaa tagttcgaac tttttccatc
    56461 tttagtaaga ccttttactc tccaaaagta agtattcttt tttaatgact ttgggactaa
    56521 gaagtttcga ttcacttttt gagtctctac tatattttgg aatgctttgt cctcagcaat
    56581 ctgaacttca taggaagaat attcaggagc tcctttccag tttaatacaa atttttcttt
    56641 ttcgattgca gttacggtag ttgctgtatt ttcaattgca gcgatttcta cagggtctaa
    56701 atcaactttt ttctcgatag agaaggtaac agactctgat actttaggtg gaacttcttg
    56761 ggaaagaaat ctcgcactca ccctactata gtatctacct tcacccaaac tatcgatagc
    56821 aaaccgattt gtatgagagt ctatcgtttt aataatttct ttaaactcga tatctttagc
    56881 gatttccaat ttataggagg atgcgtattc acttgtattc caagaaaagt taataattgg
    56941 attagtgcta atcagtggat acttagtgtt atttacaggc gagaaaaggc gaattggttc
    57001 ttcctttatg atcgagagct tgcgtgactc actcacttca ggcgttaagt tcttgctatt
    57061 agtaataata cgccaatagt atgttgaaac aggaagttta agttctagat ttttctcttt
    57121 ctttactggt atttttttta atttcttact aaacttataa tcctctgcta tctcaagagt
    57181 tgtttcgaaa tctgcgttcg atcctgtctg cacatcccaa gagaagttta ttgcagaaat
    57241 ttcatttccg agtataacag cccgatctgc tggcttattt aatttaactg gaacagaatg
    57301 tatttcaact ttttcttctt ttaatgaagc aagttgatct ttttgaatta cactctctcc
    57361 ttcttttgtt ttgattgttg catttccttt atcaacaatg atatctaatt tcttttcatc
    57421 gacctttgtt aattttacat ctgatttatt taactcgatc gttttgctac cagatttaat
    57481 attcaatttt gtatctgctt gattctcaga tctttttgca gcaaacgccc cgtaagcaaa
    57541 atcaagattg atattctggt tggaaaaatc gagaagaatc atgctatttt catccatatc
    57601 aatctgagtc ttgtcattta gctcgataga gactgttgaa ttatccgctg tccgcacgga
    57661 atctctattg gaaattggtg tatcacgatc tacggtttcc caaacaacct gtccatctat
    57721 ttttctttga atcagctttt ctttatagcg gataatgccg attacctcct ccgcgccaac
    57781 tccaattttc cgtttaaaat caatatacaa taaaacggaa aaaaagaaaa ttaggaatag
    57841 cagaacagat acaataatcc gattgctatt caatgatctc atactttact tccttttctt
    57901 catcctcatc ttccttccct gaggatggtt taggttcttc atatttgatt ccaactaact
    57961 ttcgcacagc agccaaatca gcagggcagt ttggatcgtc aaagcgacca atgactgcat
    58021 aaatagtttg gggtgcttct ttacctttca ccttgattgc tttcattttc tctactttga
    58081 aaatatcctt aacgagtaga tataaatctt cactgattaa aatatctgtt ccaaatggtt
    58141 tattcaatgt ttctacgcga gaagccaaat tcacagcatc tccaattaca gtgtattcca
    58201 ttttatcaga agatccaatt tgtcctgcaa caacaggacc ataattaagc ccacagccaa
    58261 ttttaataat tggtttctta tctccacctc taccttcgtt gaatttgaga agaacagaac
    58321 gcatcatgag tgctccattt actgcatttt cagcatcatt gccacgcgaa aatgctgctc
    58381 cccaagttgc cattacagca tccccaataa atttatctac agttccatgt gtttcattca
    58441 cacaacgaac catttcagtc atataagcat ttaaaaactc aaccacttct tgcggatcta
    58501 acttttcgga aatagcggta aacgagcgta tgtcagaaaa aaagatagca cagtttttct
    58561 tttcacctcc aagtttcaac tctcctttaa gaaccatctc tgcaacttct ttgtttacaa
    58621 aacgcccaag tgcatctttt actttctctc tttcttcaag ccctttaccc atttttacaa
    58681 atgaattagt caataaacct acttcatctc tgactgttgg tttaatatct actcggaaga
    58741 ctccttcttc aattttctta gtcgcatcta ctaaatttaa aattggactg gtcagtgtat
    58801 ttgcaaaaaa caaaacaatg agtatcgctg tgcaaagagt tataccaagt attaaaagat
    58861 tgcgacgttg gatattgtat acttcttgaa aagctttctc ttctaaaact gttgatatga
    58921 ctcctactcc tgcgaagcca atttttttat aagaacctaa gtaatatttt tttgtttctt
    58981 catgctcata acgtgtaaga ccattatcga ctgtgctttt catcatagtc ttaacgatag
    59041 gcgattcaga taaattagtt ccagataata ctatgttgga atttggatga gctaaaacga
    59101 taccttctcc attcaccatg aaattttcag aaataccagc tgttttaaaa gcgtttaaaa
    59161 acttttccat tctaaatagt gtgattaaaa tagttctaga gtcataatca cttctgtgcg
    59221 gtagagcaat tgctactatt gggtatggaa atccttgact tgcattgtga ataacagatt
    59281 ctcccgtaaa cgccttaatt aattcctttg aatgaatatt taacagtttc tgtaagtctt
    59341 cttcagagac accgttttca gaaagatatt tattattaaa attgacttgc tttgctttca
    59401 atacaccctt atcttctgaa aaaattccaa aaagcataaa attgatatcg tctttaaaaa
    59461 acgcttctac taacttctct ttatcctttt ccgaattaaa tctttgttca gcaacatttg
    59521 ccatgagtct agttttttca atgacagact caatatctgt tttaactttt aaaccaatta
    59581 cctctgctaa ttttatattc aactcttcaa ctcgaatctc gctatctttt cgaaatgcat
    59641 aactagcaat cagaatcata agagagatgg aaacgaccat aattagggat atgatggaaa
    59701 tcatttttac tcggatggta aattttgaga gtttgaaatc agttttattt gcttccatag
    59761 atttttgcga tatcctagcg atttatgaat tagtgtaaac tcatttttta tagaataatt
    59821 cttatcttct ttcgacaacc gattatttaa agtgtgaata aaatattcca atgagaattt
    59881 tgtaaaattg aaacaaacac gagtcaaaga aagaggagaa actatgagat ttatattctt
    59941 ttttttattt ttactaattc aatgcggtag ctctcaagag ttccgacaga cagttcctaa
    60001 agtagaccta aatcgcttta tgggtaaatg gtatgtaatc gcaagtcgac caacatcctt
    60061 tgaagaggga gcgtataatg cgattgaaat ctatacctac aaccaagaaa gagaccaaat
    60121 cgatatacaa tttactttta acaaagatag tttaaccggt cctgtgaaat ctattccaca
    60181 gacgggctat attatagatc gagaaaataa ttcctattgg aaagtttccc ccttctggcc
    60241 actaaaattt gattatttga ttattgacct agagagtagc taccaatggt cagtcattgg
    60301 agtccctgat caaaaataca tttggattat ggctagaact ccgactctgc ctgaaacaac
    60361 ctataaccag attttagaga gggtgaaagg tcttggttat aatttatcca atctaaagaa
    60421 aattgaaaac aaaccctaag agtttttttt atgtagttca atgattttag attgaatcaa
    60481 ggataagtcg gcaagctttc cctgcccttc atccccgcat acaaccatcc cagaagatgg
    60541 atctaaaatc tggactccat ctttttctaa gatctttaaa tttctttgca ctgctgggct
    60601 catatacatt ccaggattca tagctggggc gagtaagatt ggacaagtgg atgcaaggta
    60661 tgttgaggta actaaatcat cagcaattcc atttgcgaat ttaccaatta tgtttgcagt
    60721 tgctggaacg attgcaaaca cagaggcaat attttttaat tcgatatgcg ccatacccgt
    60781 atcatactca ttgattcgaa ccggctttcc acttaacgct tcaaatgtga ttcttccaat
    60841 aaattcggtt gcgtgttcgg tcataattac gcgaacagga tatccatctt ttgttaaatt
    60901 acgaactagc tcacatgttt tatatgcggc aatacttcct gtcaccgcga ttacaatttc
    60961 ttttggaacg ttagtcatag tcctctttgg tatgaacaaa attcaaagga gccatatcaa
    61021 aacgaatgtt taaaatttta tttccttgca ttttctctac agtgagagtt ccttttgtaa
    61081 ttgtaatgga acttccttct gatggcatat cttgatgttt ctctaagaaa aatccagcaa
    61141 tggttctaac atctttcata ccttcgattt cattttcatt gaaaatatct tctaactcat
    61201 ctagctctgc ctcaccatct atagtatagg aatcagactt aatttcaatt gcttctactt
    61261 ttacatcggt ctcatcatga atcggtccga aaacttcttc cataatatca tctactgtga
    61321 gtagaccagc aacgcctcca tattcatcta caacgattgc catatgctgt ttgtattttc
    61381 ttaatttctg taaaactttt tcaatagata aagtctcagg aacaaacaca ggagcttgca
    61441 tgatagattt tacagttgca tttttatttc gattggttct cgcaagccat tcgatcaccg
    61501 cctgcacatg aatcacaccg ataatcttat cagtcgtttt attataaaca ggatatctag
    61561 aaaaatgatt ttttgcgatg actggtaaaa tctcttttat cttttcttct gcagaaaatc
    61621 caacaatgct aagacggtga gtcataactt cttttgcttc atgttcagag aattcaaatg
    61681 tattttgaat gatctgtaat tctgcgttgt caatattacc ttgtttcgta tgctcttcta
    61741 ataatatcat caactcttct gcgctatgca caaacttatg actcaaattg ttaagcttga
    61801 gtgtcttcat aaattggttg gtgattccat tcataaaata agtgattgga taaaaaagat
    61861 aatagaaaaa gagaagtggc aatgaggagt ttagagcgat tacttctgtg ctttggattg
    61921 caattgactt aggaattaac tcacccagag tgatatgcat aaaagtgata aaaataaagg
    61981 aaatcgtaat tgacacacct tctaaattcc aactttcttt tccaggaaaa tcaactaggt
    62041 tcaaaagacc atgaagacca actgctacaa atttttcacc aagccaacca aggagtagac
    62101 tggcgattgt gattcccacc tgacaaacag atagcatatc attcagctta tttaaaactg
    62161 attttgtaat agatgccatc ggtctgtttt ctttgattaa ttcttctagc ctagaaggtc
    62221 gtaaggaaac aagtgcaaac tcagcagcaa caaaaaagcc atttactgca actaaaaaaa
    62281 gaattataaa cgtttctatt aaagccatgt ttttacaaac ctgactgttt catttgattt
    62341 ttgatttttt ataccatcta ccataataaa ttagataatt agacttaaag aatgtattct
    62401 aatatcgaga ttaattttga agactccatg cgtctagcca ttcataagat tcaatttcaa
    62461 aattgtattc acgagttaaa atttcaacaa aatttggcgt atgcgccgct ccatataaga
    62521 gccctaactc aagtggaact tgtgaattaa atttaggttt tacaaatgaa agtgcaattt
    62581 tgttcctctc tataataatt gcttccctag gaagcctcat ctcttccaaa aaacacaaat
    62641 caaaagctga tttaactaga ttttgagaca ttttcttttt aaaacttaac actttcgtgg
    62701 agtattccgc actatctgta ttttccattt gcataagttc agaatagtct tttgaaatag
    62761 atttactatc attggaaaaa ttggtcgata aagaatcaaa tcctatttta tagtttttta
    62821 acatttctac aaattgctgg aagtccatat ccgcattgat ccagttctgc tttggtttta
    62881 acaactctga ttgatctgca aaatttaaaa atcgagctat ttctgacttt aaatcggaat
    62941 attttttaat gcttcgtcga gtgtccatct cttcgagagt ctgtgcataa taatttttag
    63001 aaaagttttt agaatgcatt ttagaactaa cgcgaatacc ttcataatat aagaaatcta
    63061 attcattgat tatcttttga atatttaaat aatattcttt atcagcaaca tgaactacac
    63121 caatgatctt caaacgaacg ttagtgtctt tcgaaactaa tgtatacact gaagtttgaa
    63181 gagcagtttc tgtagctctt aaaaaaggaa tttctttgga ggactgagaa taaatatcta
    63241 gagcaacaaa gaataggaaa aataaaatag ataagcactt caaagttgca atccgaatca
    63301 agaacatact ctcatagtat tcaatcgttc caatttagtc actcaaattt taaataaaaa
    63361 taaaagcata gaaaatcaaa aatattcgat ttgacgaatt ctagaattcc tttttaatta
    63421 gcaaaactca aatccatgaa tgaaacaaat tttacatcaa tacaacgcaa ttcaaccctt
    63481 gatgttctga gaggcatcgc cattgtttca atgatactct ctaattttgc tggagaactt
    63541 ttgaaagcac ctcatccaat tcttgttaca tggattggtt ctcttgctgc cccgctcttt
    63601 gtaatgattg ctggatacct tgttggattt tcagttacga ataaaaatta tccattctca
    63661 tactttctac tccgaggttt tttgacactt ctaatcggtg tatgtattga tgtctttttg
    63721 tggagaagtt atccatttat atctgtagat attctctact taactgggtt atcaattccg
    63781 atcacatctc tctttatgcg aatgaacctt tccttcaagg ttgtttttat ttttttattt
    63841 tttatctttg gtcccctact tagaatgcaa tttggatatt ccgataaacc aagttttcca
    63901 cccatttgga aggatgggaa ttttgttcct tttgcgggag atttctttac aatagtcaag
    63961 cattggttta ttgatggttg gtttccaatt ttttcctgga tgggagtttc ctttttaggg
    64021 ggatttttgg cagagttaaa aataaaacac ggagatttga tttccaaaga taaaatctat
    64081 ctattgctat tgagtttctt tttattacta gtaggagcct ttttttggaa tcatgagttt
    64141 cgtttagcaa cacgtgggaa ctatacagag cttttttatc ccccaacatt gagttactat
    64201 ttagtgtata ttggccttat tttcatttta tatcttattg tagatattaa ttccaactct
    64261 ttcctgtatt cacccttccg actaatcgga attagccctc tgttttttta ccttcttcac
    64321 tttctaataa ttgctattgt aattgcaccc ttctttggaa agacaccaga aaaagaagta
    64381 tatttagatc taaatgtatt tctaatttca tttttattta ttattttagt ttgtatttta
    64441 ttttctttct ttatttcact cttcaaaaaa aagttttcca accttccctt cttagttaaa
    64501 ttttttattg gaggataatt ttaacaaacg agagaaatat ttttctatca aatctataat
    64561 cccctattat tcaaaatagt tctccaaata tttggaggtt ttatatatat atttgtttcg
    64621 ataatgattc taattggatt aatttttagg aaagaaattc acttcaaaaa taatttgtag
    64681 tttcagagtt ttacttttct tattacgaat ctatttacaa aaaacgtttt agaagaccca
    64741 gatatacaat ttctactaaa ttctaaaaaa gggaaataaa atcaatctat tgacttttat
    64801 attttctatt tcacgtttat tggattcatt ttatttagga aaaacgaata tcaccatttc
    64861 attctctgtt ttagtttttg gataaagcat agatgatgtc gaccatgcca agcatataga
    64921 ccaatcgtcg gtgccaattt aaaaaacttg ttgtgttctg aatgaaaaaa agatttttct
    64981 agatcgtcta acgttagaga tctaagtaaa acaacccatc ttttatgcaa accctctaaa
    65041 atttgaagag aactttcaat gtttgagtcc tttccatcta tcaattcagc ccaacgatct
    65101 tctgcatagg gtttaatctt aggactatcc tctgtgagag ctaatttaaa acgaataaaa
    65161 ctattcatat gactgtctgc caaatgatga attacctgtt ttaccatcca accgtctggt
    65221 tttgtgcgag tagaaagttg ctcctctgtc caaccttgaa tggtttctct taaaagtttt
    65281 ggaaagtgtt caatttcctc aatccaacct gttatatgat cattcgaaat gatatcagga
    65341 tgagaatact cccctacagg ataattttga tttgcctcat tttgtttatt catagtttca
    65401 tcctttaaaa tattcattca ctcagattat aaataacgat tgtaaagtaa actgaaaaac
    65461 ttttcagtgt aagaagtgta ataaatctta gagaaaaagc gaatgaccca aataattaaa
    65521 atactaatca tttctttatt atgtatttca ttctgtaatt tgaatgcact acataataaa
    65581 aaacaagaga agaaaacgaa atcagcaccg aaaaaaaaac tctatgattg taaaaattac
    65641 caccaaggga aatttactta cacagatata aacggtttaa aaattctgat cattcgggaa
    65701 aatgaaattc aatatctata cagagaatac ccaaacgcct ataaaaaaaa gagtatagta
    65761 tggctaaccg attgtaagta taaatcaaca gtgatcgaaa tccatgatcc aattttagat
    65821 gattcaatgc tcggaaaaga aaatatagtt gaaataacca agaatcgtaa ccaagactgg
    65881 tatgaatatt caaattcgga aaatgatgtt ctatatacgg gaatcgttta cgtagtcaaa
    65941 gactgatctt caaaccatat tcttttacaa tcgttagtaa cgcttctgcg ttcccctttg
    66001 catcatcaac aggattgtgt gtgtgctttg tctttcttaa atgcttaaaa ttttgaaatg
    66061 tatttttgac aattcctttg taaagagatc ctaaatttgt agagctatgc ccgaaaggat
    66121 tacttccata aaatcgatga aaataataag ctataaacat ccagtcaaat ccattattat
    66181 ctgagataaa cattggttgt ccctttgtat ttttcttgat ccagtcagaa aattctttca
    66241 taacaattgt tggctcagaa taagaaagtg tttcttctct agtgacgtta gacacagcta
    66301 aagcatctgg gataaactga tttgagattg gttttatttt accgtagaaa gttttgtcta
    66361 gtgtagaatc cactagaaca gcgccgaagc tgatcattga atattcgccc gggatcggac
    66421 catcagcttc gacatctacc atgatataac tcattgggtt ttaattattt gggtgctcga
    66481 ttccttttcc atcatttaga aatttagaaa tgatcactct ttgaatttga gatgttcctt
    66541 catagatttg gaaaatcttt gaatctctca ttaatttttc taccggatac tcagtgttat
    66601 acccatatcc accaaacact tgcactgcat ccgtgcaaat acgaacacac atatcagcgc
    66661 aaaatgcttt tgcaatcgat gcttggtaag tatttttatg accattgtca atcatccaag
    66721 cagcttgcca gcaaagaagt cttccggctt ctatgtcttt tgccatatct gctagcataa
    66781 aactaattcc ttggttagaa gaaattggtt ttccaaatgc atttcttgta tttgcgtatc
    66841 gaatcgcatg ttccattgca gttcttgcaa cacctactgc gcctatcgct actccaggac
    66901 gagtatggtc aaatgcaccc atcgcaattt taaaaccatc tccctcgttt ccgatcattt
    66961 ggcttttatg aacttttaca tcttcaaaag tgactcctct tgtatcagag catctttgac
    67021 ccatgttctt ttcttttttg ccgatgatca ctccaggagt tttggcttct acaataaatc
    67081 cagtcattcc cttatgacca gctgctggat ctgtttttgt taaaacaaaa aaccaatctg
    67141 ccactccagc atttgtaatc cacatttttg aaccgttaat cacatactca tcgcctactc
    67201 gtgtagctgt agttcgaata ttcgcaacat cagagcctgc ccctggttca gtcactgcat
    67261 aggctgctag tttaaattca tttgtcatag gttctacaaa tttcttcatg acttcgtcac
    67321 ttgctccgat caaaacggga gctaacgcaa gattatttgc taatatagct gttgccattc
    67381 cagaacaacc agaatacaat tcctcgccca tgataacatc atctagatct cccatacctg
    67441 caccattgta tttggttggt atatgtaagt tcatgagacc agtctcccat gcttttttga
    67501 gaatatccat tggatattct cctgttaagt catgatgctc tgcttttgga gccatttcat
    67561 tttttgcaaa atcgcgcgct aaatcgcgta acgctttctg ttcatcagtc attcggaaat
    67621 caatcatcgt tatatccttt tcatttaaaa aaaatagaaa ctactgatat attttttgaa
    67681 atactatctg tgtcaaggtt tcttctcagg ctttttagtt ttgcgtagac tcgttttgac
    67741 cttttcttta attggaatat tcattttacc aagcatagtg ttaaactcga cagatccctc
    67801 caaaataaat tccatttccc ctcctgctaa gactgccttg attaaatcag ctaagaatat
    67861 aaccaatttt ttattcacgt tttcttcgaa tagagtttgc atatctgctt gaatgatttc
    67921 tgtagacaag gctggaatct ctttctccga tgtatttgta acttctgcaa gataggcatt
    67981 ttcatttcca ctctctacag ttgaaatttt aaaagagaat ttctcaatag ttacaggaac
    68041 atcatttgta ttttctattt gaatatttgc tttaaaatac aacttgggaa ctggaggaac
    68101 gataaacata cggacatttt tgatttctaa agaatcgact gttattttgc aattttccaa
    68161 ttctcttctc ttcttatcaa ttggattcga gcaggcatta aaaaggaata gacaaaggag
    68221 aatactgagt tttaatttca ttgctgtatt ctttgtttgt attaaaattt ggcaaatggt
    68281 tttttataaa attctaagac tttataaaaa catttttagt ttacacacat ttagagtttt
    68341 aaaatacaat ttgatagcct gctgttttaa acattatatt tataatatta aatttttttg
    68401 ggaatatttt aatgataaaa aagaaaatac gattctttag tagcaaaata ttattaactt
    68461 cgattcttct attctcactc aatcaatgtc tacctaaagt agaattttgg ataataaatg
    68521 attcaagcta tgactataaa atagaaattt tatttaaaag aaaatatact ctcgaaggat
    68581 ttggagcatt gtttggatat aaattagaaa aaatgccggc tatcgcaaaa tttccatccc
    68641 cttcctatga tgaaatgttt tttcaaggcg acagatttaa aaaagatgct gagagaatca
    68701 cggaagcaga tttccaatat gatccaaaca ctgctgtttt ttcttataca ttaaaatcaa
    68761 aaatgtcttt cttgctagag agatcgagcg aattagaaca ttcaagagga attgatctca
    68821 ttcaagaatt gagatttatt tcaaaggatg gagaaattat ttataaaggc aaggaaatta
    68881 gaaaagcatt tttattaagt aaggaaatca caatcctact taaatgattc actacaaaga
    68941 ataataagat tatctttgag ctttcgcaag cgcaatgcct cttggattat ttactccgag
    69001 agacaaagaa ctgtttaccg ccaaattccc gtttggctga atcgtaaaaa catttatctt
    69061 tccagacaaa ccagctacat cttccgtggc aaatagatac ttaccttgcg gatcaatcag
    69121 tagatttcta cattgattgc ttggaatact atttaataat gagggagaag agttagaatt
    69181 taacccataa gaataaatcg tattaaatgt aagactacaa gcgaataaat actttccaga
    69241 aggatcgata gttatagttt ccatcaaatt agcatttaca acaagttcag aagagagaga
    69301 tccattgagc tgattgatcg taaatcgaga tatcccgctt ccagcaattg cattggcagt
    69361 atacaaaaaa ctaccgttag gctcaataga aataccaaga gggtttgctg aaacagaata
    69421 aggtgagcct atcatcggat ttaaatttcc agtattaaaa tcgattgtat aaccatacac
    69481 ttttgagcta gtattggctg taacgtatac aaaaggaaga ctaggatgag tcacaataga
    69541 ataaagtcct gcaacaccaa catttgtcgc acctaataca accacattcc ctgtattctg
    69601 atcgattaaa actctagaaa ttgtcatatc agtagaattt acaacaaaca ttaaatttcc
    69661 agttgaatct atagcaatcg acttgagacc atttccaact gtagctgtat ttacacctgt
    69721 gagagtgcca tttgtttgat tgatgctaat attatttaaa actgtagatc cagaattaac
    69781 ggcatacaat gatttgccgt taaatgcagc tactagggat tgtggattag tccctgcagc
    69841 aaaggaaact agattagaga aagatccatt agcagaatcc agctccatac tactgatgga
    69901 atttgaccca ttgtttgcag tatataggaa ataacgatag atataaaata tatttttaga
    69961 atttgccata ccaaaacgat tgactacctt tatttgacca cttgctgcac cactaggcac
    70021 tacaaagctc aaactagaac catttgaggc aatcccattt gttgcaattc catttacaaa
    70081 ctgaattgtg tttgcactcg gatctaaatc aaaattcgct cctgttattg tgactgtttc
    70141 gcctaccgca gctcccggga gatttatttt ttgaataatt ggtgcagaat agggaactcc
    70201 atcatccccc gtgcagactc tcgcggtatc aacggttgta taagtctccg ttgttacatt
    70261 tgcaccgtat gtgaatattc tcttagataa aaataaattt ttatctaaat aagcttctgt
    70321 tttttgatcg gataatgtag ttgaaataaa atagcaaaat aaatttgtat ttggatatgt
    70381 ctgagaaaat tgtagtgttt gagaaaaaat taagtttgta tcatcatatg tatttacaaa
    70441 tggaacagag caaacactat tagatagcat aatgtttccc tttataactc tatttttaga
    70501 gtcatattct actccattca cggtagttcc atccgattga ataatgttta gtaaccgcat
    70561 agaattatcg taattaaaaa acttatttgt agaagaatcg ttattaattt gcgaatatcg
    70621 catagtatag attgtaaaat tttgaacccg atttataaaa tcaaaagtag aattatagaa
    70681 ttcagttgta acagttgtgc ttatattatc tactgtaatt ggatttgtat agatacagtc
    70741 ataggataat ctagatatat cgaaattaca tgtataggaa ttattcgaac cagaagagtt
    70801 cgttgagttt actgtttttg cataggtgat acaagactta gaactaaaat atcgataaat
    70861 ataggaaaga aatcctatcc ctccaagact tttattcaca tgagtattgc agtccaaaac
    70921 aaaaatagtt agaacaaaaa aggcgattcg attgaacatg gggatgattc ctccattggt
    70981 ttattaaatt acacaaatta caattaagct aagtatagtc agtattttga aaatatataa
    71041 aagcctctca ctacaatcaa tttaacatat cctaaatcaa tctttataga tttgaataca
    71101 ataattcaaa aattctacat atagcctttc cattgataca tcaggattcc catccactct
    71161 ttaatggcga tggttgtagt ttccaatgct cctgttgcag ggattattat ttcccaattc
    71221 aaatccatcc taagagaacg ataatctgtt ggaaatgggt aagcttctat acccaatttt
    71281 tgaaaaatag caatcgatct cttcatatga aaggcagagg taacaagaat tatcctctca
    71341 atctttttct cttctagtat tttctttgta tagagagcat tttcaaatgt atttctagat
    71401 tcagattcta gaagtatttt attttcagga attccaaaat caatcagaaa ttttttagca
    71461 aagccagctt cttttacctc ttggtcaaat agtaaacctg aaccgcctgt aaacaaaaca
    71521 tactctgcct tcccagcttt atgcaagagg atagcatctg ttagccgttc accagaacct
    71581 aatatttcca ctcgatccgg aaaaggactc agagtattaa tcattccacc taatactaca
    71641 attgcatctg ctctaggagc atcttttact tttataggag gaaattcttt ttctaaagaa
    71701 gtaatcaaag actgacaaac aggaaaacta gcggcaaacc ataataatcc gattggtaaa
    71761 aatgataaaa atttatttct tccccctttg attttaaata aaacaatcaa taatccaagt
    71821 aaaatacata aaggcaaagg aaataaaatt acagttagta tcttggagaa aaagaaaaaa
    71881 acgttcatcg gatatggtct ttaatttttt gaattgtaaa atcaggcttt tcaaaaggga
    71941 aaaaatgggt tccatctgga agaattgtaa gggtagaatg tttgtttcca gaaatgataa
    72001 tctttgctct ataaggagaa caaacctcaa acttttgcgg aatgataata tgagtctcta
    72061 atttattttt tccataatct ttgaaactct ttagagatgg tgttttaaaa tattttgctt
    72121 cgattgtagg atcacaaata agctctattt cctctccttt ttttctgaag caagagcgga
    72181 gatagtcttc aaaaactcta ttgtcccaat tggaaaaagc agaaaatttt cgaaaggatc
    72241 tacgcactac ttctaaattt ttaaaatgcc ttctccgttt tgcagcattt tgagaaattt
    72301 gactgccaaa tatttttcca tagacgataa taggaatact caaaaaggtt gggtctagta
    72361 aaaacatttt tttaaaatga gaagtttttt tcttagaagc cctaagagca ctagctcctc
    72421 ccatagagtg accaatggag atgatacgat ctagattttc tagttcaatc agtgcaagaa
    72481 tttgatctct caaaaaatcc caatttgaaa aatcataata aggatcagac tctccatgcc
    72541 ccgcaaaatc aagagcaatc actctgaact catttgcaaa tgcctcaatt agataagaat
    72601 agcattctgc gctatatcca tttgcgtgcg ttatgagtat aatgtcctta gaatttgttt
    72661 ctgtatcaat gtaggatagt tttaaatttt cgaaagtgaa aaactttctt ttaaaaatta
    72721 tgtttggttc ggactttgat gatttcactg ttttccttaa aatctatgat tgcattttta
    72781 ttttctgttt cccaaattcg aaaacttctc tagtgaattc taaatatcga aatacttctt
    72841 ctactttagg atcattttta atatcatcat aacgaacaga cattccaata tcatttacat
    72901 ctgcataaat atcctgagaa aacgatttga actcttgttc caattcagaa cattttccta
    72961 taagataaat caagtcatga ttcgatttaa tttttacatt gtggtatgtt aaatacgctt
    73021 ttaaagactt ttctattgtc tgctgacaat gaaatgtaat tgtatctgta tattccggtt
    73081 ttttctcgta aattaattca gctgatccta aatcatgttc tgctttgatt aaaaaatttt
    73141 taacaacgtc tgttttcaaa tttcaattcc atctttgaaa atttcattat aaagagtatt
    73201 catttgcatt ttataggctt caaactcttc taaatttttc actaatatat caaaaggcac
    73261 atcaggaatt tctttatgtg catttaaaac gatagttctt ctatattttt tcattaattc
    73321 accatcgttg atattattta gcagaaccaa aaaatcataa tcgctttcta gactttgatt
    73381 tcctttcgca cgacttccga ataaataaat tctatttaca ggtgcttcga gtttataaac
    73441 taaattttta aatttaataa cagtctcatc attaagagta atagcttgat ttatcatata
    73501 aatctacttt cttttaaaaa ttatgtttgg ttcggacttt gatgatttca ctgtttattt
    73561 ttttccaaag tgcatcttta tcaggatgaa aagttgcata aacacctttt tttacaagat
    73621 cgatcagttc ttcgattgta aaattcaaaa atcgatagag tttaaaatat tcatatgaga
    73681 gatttacatc aaatatttca ggatcgtctg tattgataca agtataaaca cctttatcat
    73741 agtattctct aacaggatga ttttcttctt tcttaacata tttacctgta aagacgttac
    73801 tcgtaagaca tatctctatt ggaatttgtg actctttcaa atactcaact aagtctggat
    73861 ctaaaattgc cgatgttcca tgtccaattc tttcaacttt taaataattg atagaatccc
    73921 aaatagacca aggtccatca tcttctcctg cgtggataac cgcgtgtaac cccgcctctc
    73981 tagctttttt aaacacttct tcgtaatctt tggcaggtcc aagtaactcc gcaccaccga
    74041 gaccaattcc caaaacttct gggtatttta gatttaatac acggtttagg ttgttcattg
    74101 cattttctgc accaaaggtt ctagagacat cgatgagaag tcgaatctct aagccttctt
    74161 tggctttgag ttcacgaata cgagttacca tgacctcaat cattttatca aaatcgattc
    74221 cattttggat aaacttagtt ggagcgacaa aaacttcggt ataaacgata ttatccgctc
    74281 gcatatagga tgcgagacta tcaatcataa acgaaaagtc ttcatgtgat ttcaccgcac
    74341 tctgaataaa taaaaataat tgaatgaatc catttaaatc atgaaacatg aatttttttt
    74401 caaattcttc ttctgtgatc tcgaccccat ttttcttaaa catttctttt aacgtttgtt
    74461 tattgacgca agcttctaag tgtaaatgta tttctgtctt tggaagagct ttaataaatg
    74521 ccataacctc ttgttcagtg ggttctgtat ttttataatt gggatcaatc gttggtatgg
    74581 tttgaatcac ttcatgcttg tgatgaccat tcgttacgtc tccgactaac catgcgggag
    74641 gatttggaac tgtttgctct aatagaacat ctctttcatc tagtaaatta tttatctgct
    74701 tatcaaaaat aagttgtaaa ccagaagaat aaggacgatc gtcaggtagt ttgcttttaa
    74761 tacgatttag ctcgtttaca tctctatcta tgatagaaat tcgattgagt atttgttcaa
    74821 actttgcttt cacggttttt agtcaaaaaa taatttacta acgagaccat tatatttact
    74881 tattgtatcg tatctagcct atattagtat ttaaatatat ttttgtagaa agccttttat
    74941 tggcaataca gattcaattt tcagttcaga aatttttttt taggacgaaa tttagaaaaa
    75001 ggattagaca tagtatgaga accatgagaa agaccttcct tccctttgct ttaccatcca
    75061 tatcagaaga ggctatagag gaggttgcag ctgttttacg ttctggttgg gtaacttccg
    75121 gacctaaagt aaaacaattc gaaagtgaat ttagagattt cgtaggaacg aaagacgcaa
    75181 ttgcgttaaa ttccgcaaca gctggtcttc atttggctct agaagcaatt ggaatcacag
    75241 aaaaagatgc agtgattaca agttcagtca cctttacagc atccgctgaa gtcatttgtt
    75301 attttggagc atctcccctt ttaacagatg tggatccgat tcacaatata atgactccag
    75361 atacacacag agacttgatc gagagagagt gtcaatggaa tggaactcat ttgattcaca
    75421 aacaatctca aaaaacgatc aaagcagtca ttcctgttca tcttgctgga tatacctgtg
    75481 atatggaaag aatcatagaa attgcaaaaa gttacaatct gtttgtaatt gaagatgcag
    75541 cacatgcatt tcccgctaag cataaaagta aaatgattgg tgcttgggga gattttacag
    75601 tcttcagctt ttatgcaacc aaaggcatta caaccggaga aggtggaatg gtaactacga
    75661 atcattctaa ggagtcagat agaattagac ttatgcggct acatggtatt aaccgcgatg
    75721 cttataatag acccggctgg tattacgaag tagtagatgc aggatttaaa tacaatatga
    75781 cagatattgc tgccgccctt ggtatcgttc aactccatga atctgtcgga ttttggaatc
    75841 gcagagaaga aattgctctt aaatacaatg aagctttttc cgatttggag ggaatgaaac
    75901 tgcctcttcc cgatgaaaga ggaagacact cttggcattt atacagaatc gaactagatc
    75961 cctacaaagc gaaattaggt cgagatagtt ttgtagaaga actcaaagaa agaaatattg
    76021 gaacaagcct tcatttcatc ccaatcttcg aacaccctta ttacaaaaaa acctatcagt
    76081 tcaaccgtaa aaattttcca aacgcttgtg caatgtatga caaagcagtc tcccttcctc
    76141 tctttgccgg catgacaaat acagatgtag aagacgttat acacgaagtc agggaattgt
    76201 tgaagtaaaa aagattacca ccgatgaaca ccgataggca ccgatgggtg gggttggtat
    76261 tttttttcac cacgaagtgc acgaagaggc acgaagggtt taggtatgat tatgatcctg
    76321 tataaattat ggtgagcagg tcacgcgacc tgcgttactt gttgctttgt tttttttacc
    76381 acatgcgaag gcgtaggtcg cgaatacttg gagcgaaggg tggattaaac accgataggc
    76441 tccgatggtt tgggttatga ttattcaatc catcgttata atttttcaac catacttcga
    76501 ctcctctcag tataaactcc gtgcacgaag aagcnnnnnn nnnnnnnnnn nnnnnnnnnn
    76561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    76621 nnnnnnnnnn nnnnttgata gcatggaaca aattcataat cttttcaatt gcaccttttt
    76681 ctttcgtttt taatccacta tccttttccc attgacggaa ttctctagtg agtttagttt
    76741 gtttttttgt gagtgaacga ttcacactca ctaagaggtt tatatgtatt caaaagagga
    76801 agcacttgca aagattacgg ttttggttaa aagattcgaa gctcagatag atacatatag
    76861 aaaatccgat tacaatgaaa cattaacacg aagagatttc attgatccat tatttgaagc
    76921 tcttggttgg gacatagaca acaaacaagg agcatcggaa agttaccgag aagtaattca
    76981 tgaagataaa atcaaagttg gtggtgcaac caaagcgcct gattattcct ttcgattgac
    77041 tggcggcaaa cggctatttt tcgttgaagc caaaaaacca agtgttgcag ttaaggatga
    77101 tatgcttcca gcttaccaag ttcgaagata tggatggagt gcgaaacttg ctatttccat
    77161 tatcactgac tttgaagagt ttgctgttta cgattgcacc aaaaaaccca acaccactga
    77221 taaagcgtca gtcgctcgaa taaaatacct tacatttaaa gaatatattt ctgattttga
    77281 ttttctttgg aatacattta gtaaagaaaa agtaatccag ggaagttttg atgcttttat
    77341 aaaaaatgat acaaacaaaa aaggaacaac cactgtagat agagaatttt taaactcctt
    77401 agataattgg agaactattc ttgcaaccaa cgtcagtaaa actaatttag aactaaatga
    77461 agatgaaatt aattttgcag tgcaacagtt tatagataga atcatatttc ttcgcattgc
    77521 agaagacaga gcagtagaag aatacggaag tttgaaagag gttataagta agccaatcgc
    77581 taaagcgggg gacaatgaat attatacaaa cttagtaaag ttatatatga aagcagatga
    77641 taaatacaac tctggaattt tcgattttaa gaaagataaa atttctacaa gtctcaagat
    77701 agataacaaa gttgtaaaaa caattcttac agaactttat tatccagaat gtccttacga
    77761 attctctgta ttatccgttg aaatattagg ctctgcttat gagcaattcc ttggtaagca
    77821 aatcaaaata gataagtctc ataaggcaaa gattgaagaa aagccagaag ttcgtaaagc
    77881 gggaggggtc tattacacac ctgaatatat cgttgaatac attgtaaaac aaacagtagg
    77941 taaattaaca gaatcaaaat caccaaaaga aattgagaaa atcaaaatcg ttgatcctgc
    78001 ttgtggttct ggaagttttt taattggggc ttatcagtat ttactcgact atcataaaaa
    78061 ttattatgca aaaaataaaa aatcttggtc tctcaaagat tctccgatca caccagatgg
    78121 aaatttaacg acagcagaaa aaaaacggat tctattaaac aatatttatg gagtagatat
    78181 tgatgtaaat gcagtagagg taacaaaact cagcctatta ctcaaatgta tggagggaga
    78241 gacagaagca agtataaaca accaactccg attgttcaat gaaagagttt taccaacgtt
    78301 agacgacaat attaaaagcg gcaactctct aatagacaca gatttttatg ataacgaact
    78361 cgactttggg gaagataaaa aaataaaacc tttccactgg caatctgctt tctcacaaat
    78421 ttttaaacag ggtggatttg acgcagtgat tgggaatccg ccgtatggag catacttcac
    78481 ttcgaatgaa acagactatt ttttacaaaa atatagactt caagattatc aacttgatag
    78541 ctattttctt ttcattgaaa aatcacttga gacattgcag gataatggat tgctaggttt
    78601 tattattccc aatacatggc tattaaactt aaatacatca aagattcgta ttcatttatt
    78661 ctcgaaaaca gaaattacta atattgttca ttttttaaat ccagtctttg ctcaagcagt
    78721 tgttgatact gagataatgg tttttagaaa aacaaaacct cgtaaaaccc acgaaataaa
    78781 aatagagatt tatgataaga aaaatgaaaa agtagaaaat aaaataaaac aacagaactg
    78841 gattgattcc aatggtgcgc cagttaatat ttttgataat cctaacactc aaaaaattaa
    78901 atctaaaatt gccaatctcc cgattcttga tgcagtttgt aaaattacac aaggaacgaa
    78961 accatttcaa gttggaaaag gaattcctcc tcaaacaaga acgatagttg acgaaaagcc
    79021 ttttgtctcg gaaaaaaaaa ttaatcattt atttcgacca cttctaagag gaagtttgat
    79081 gaataggtat caaatactat gggacaataa ttattttata aaatttggtg attggcttgc
    79141 tgaaccgcgc tactcggctg agtatgatgc gaaggaaaaa attattattc gtcaaacagg
    79201 cgatcatcta aatgccactc tagatacgaa tcaatttatc gtaagagata atctgtatac
    79261 aattgttaat aaaatatcaa agataaacct taaatttatt ctaggtttga ttaattcaaa
    79321 atttttaaat tggtattatc aaaatattgt aaatccagaa aaaggtgaag cattagcgca
    79381 agttaaaaga gggcatctag cctctttacc aatcaaaacc atcgacccaa aaaacaaaac
    79441 agaaatccaa ctccatgatg acattgtaaa aaatgtagat ttacttttaa aattaaacga
    79501 ggagctaccg acgattaaac tccagacgaa aatagaagaa attaattcta gaatcgagag
    79561 agcagaagag aatatcaacc aagcggttta taagctgtat gacctaacgc ctgaagagat
    79621 acaaattgtg gaaggcaaag attgatctac tcaaaagagt ctgcactgtg agcgtagaga
    79681 cggttaggat tgtgaaacaa atgtctgaac tgtgattttt atgattcgag tgattaatat
    79741 gattaggaca caagagcgac ccaatagaat aatcacggca atcataaaaa tcaaaaaaat
    79801 cacagttcag acagtgggta tgattaggac acaaaagcca ctcaatacaa caatcacagc
    79861 aatcataaaa atcaaaaaaa tcacagttca gacagtgggt atgattagga cacnnnnnnn
    79921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    79981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngcaatca taaaaatcaa aaaaatcaca
    80041 gttcagacag tgggtatgat taggacacaa aagccactca atacaacaat catcccaatc
    80101 ataaaaatca aaaaaatcac agttcagaca ggaggttctc ttgataaacg aagcctataa
    80161 atactccgat ttaacttcta agataattgg ctgtgcgatt acagtgcata agacgttagg
    80221 aaatggattt caagaagtaa tatatcaacg ggctttggag attgaaatgg gcgttgccga
    80281 aattaaattt cagcgtgaat ttgaaatgcc aattcattat cgtagtaaaa gaattggaac
    80341 tagacgagtt gactttctgg ttgaggaagt tatttcagtt gaattgaaag cgattactaa
    80401 attagaagat gttcactttg cccaagcgat taattatcta gaggcatata acttggaaat
    80461 tggattattg ataaactttg gagaaacaag cctgaacttc aagaggttga caaataaaaa
    80521 atttagtaag gagaatatct aatgcgtgtt taccttgata actgttgttt taatagacct
    80581 tatgatgata ggagcataaa atgattacgg atacagaatt aaaaatgaaa gcttatggag
    80641 cattaagaga aaaattaaat cttgtagatg ttgagagatt tatatctatc attcaaaaag
    80701 aaaaatttga ttatacaaaa tggcgagaaa atattttaga agatttaagc ttagatgaat
    80761 tgagtgataa agctatgaat tattataaga aacaaaactc tccttccaag aaaaaaatca
    80821 aaaaaattaa tagaatgaaa aaggaaagat gaaaaagaaa ttagcaaaat gcattcattg
    80881 tggaattgga aaaacaagta ttaaatcagt agaaaaaatt ctatctagca ataacaatac
    80941 ggcaattgta aaagtaaatg ctgaggtttg cgatcattgt ggtgaaaaat tctatgaacc
    81001 agaaacagtt agatatttag aaagaattaa aaaagaacta aaaaataaaa atagcaaaca
    81061 attaattccg attggaatag cttataaagt tgctatgtaa attatcatca ctgcatactt
    81121 tactaatgca caggaggaag tatgagaact gatacagaaa tacgcttaga gggaatgaaa
    81181 atattatttt ctaatatgga tatagtagaa gctgaaaaat tcatttcaat tattcaacgt
    81241 gataaattcg attatacaaa atggcgacaa aaattatatg aaggactttc tgttcgagaa
    81301 ctaagtaaaa aagcaatgca tctccgagaa aacaaagaga agtgaaacgg ttaaaaattg
    81361 taactgtttg aaaggaatta gaaaaaatat ccagcggata aacaaaagta tccgctcatc
    81421 tgaaaaacta tctaaggtaa aatttgattg acttctaaaa atagaaagcc tacgctagaa
    81481 ccaatggaga agtaaatgag ttttacattt ttaatttggg ttgtaattac ttttattggt
    81541 tgtggagttc ttttatattt ttctattact gacaaaataa aagaaaaagg cacgagaact
    81601 taattagata ttattaactc atcacataca aacttcattt ttcttttaat atttaattct
    81661 aaaaagaata tacatataca tcccaaggct gttttcagac acaacaatat cattaggcta
    81721 aagatttttt caaccacgaa gaatcatgaa gcgttaggtt ttaaagtgat tattttagtg
    81781 ttcatttatt taaaattaaa tctccggaga ttttcttcaa aaaaagtatt atagattttc
    81841 cttaattcgc ttaagagcat ccagattttc agctaaataa tctttagcaa tacgccatat
    81901 aattccgtaa tcgactccaa agtattcgtg agaaactcta tttcttaatc catacatatc
    81961 gttccatgga acttctggat atttggatgt gattgaatcc ggtaggctat ttgccgcttc
    82021 tccaatgatt tcaaaatttc taattactgc atcttttacc atattactat tctgaaagtc
    82081 ttcaaagctc aatccttgcg tatactcttg aatattacat atcgcttaaa tcatatcctc
    82141 aagataaagc aaattatctc tatcttcttt tttcatatat ataccaaacg ccaaaagaaa
    82201 cttcccacaa ggattttatt ggagtaacgt ttggtataga ccgaacactt atttgtaatt
    82261 gaaaaatggg aaattaattt tggcgttcga tataaataac ctgttttaaa attctttcct
    82321 tcacttgcgg tttaattgct tctggtgtaa ctaagtctac ctttcttcca aaataatttt
    82381 ccaaaaaccg attcatgcga aagaattccc aacctggcgt ctctgtgaat tcaactaata
    82441 tatcaatatc acttttatca tgaaaagttc catttgcaaa tgaaccaaaa agtccgattc
    82501 gttttatttt atatttctca atcaattcag gctttaaaga taaaagcttt tctttaacga
    82561 aagacaggtc tagaaaattc tcaggcatga tacttcatat tttccaaaac tatttagaat
    82621 tggcaactag cttttaaatc aaaaacgatt tcattcctta aaagaaaatt ttttcttcgt
    82681 gtttcttcgt gcccttcgtg gttaaactct ctttttctta gctcaatgca tactactaat
    82741 aaactcccat tccctacatc cacgcttatc ggtgtcaatc ggtgatatcg gtggtaatct
    82801 tttaattttt tctgaatcaa tcagattctt agaagattag aaagccctta cataaaggta
    82861 agtcattaaa aaaaatactt ccaaatagaa agctttaatt tcctatattc ctagtgaggc
    82921 atttttaatc tatgaacaaa gaaaaacttt tcggaatcaa accaaacttt ttgacagttg
    82981 cgtattctaa ctttatcaag tcagcaatat ttttacgtta ttcgatcaat cctgaacaaa
    83041 agattttgct tggttccaaa ccatctccta tcttaaaaga tttagaaatt tttcaaaaag
    83101 gaaatggagt aaaagttgag aatggaattt tagtcggaac tgttcgaatg ggatatggtc
    83161 atcatagaat ggcttacgct ctctactcat gggttcttgc aaaaaaggtc aaaccctatt
    83221 tacacgacct actcggaatt gaatccaatg aagcagttgc catcaaagaa atcgacggac
    83281 tctatagcca aatgagtcga ctatcgtctg aatggggtgg aattgtagag tgggcttggg
    83341 gacaaattac tgcccaagga aatattggat ctctttatgt ttctacgatt cttgcagaaa
    83401 gttacaccaa tatgatgtct gatctaccta gatcaatgcc ttatatttcc acttatcctc
    83461 tcaacggaca aattgcagta gaagctggat ttaagaacgt tatacatctc attccagata
    83521 attttccaca atactaccta cttgttccgg gagcaatgaa cctagtccaa agtcctgcat
    83581 cttatcataa gttcattgat atgggcgtgc caaaagaaaa tttacagatc gctggtcatt
    83641 gggtatctga accaatcgtg acgaatgtag aagcagattc taaagctcgt cttgaaagaa
    83701 ttgaaaaaca aaaaccaaag agacttttac ttgcgatagg cggtgctggt gctcaaaaga
    83761 actatacaat taacttcatc gagaaaatta aaaataaatt aaaaaatgga gaactccatt
    83821 tatttataaa tgcaggcgat catggaactt tatttgatac cttaaaagaa gaatttgaaa
    83881 agatgggatt gcattacaat atagttagta attggaacga tcttctcgaa ttctgcaaaa
    83941 caaatactat tgattcaacc actgaatcta aacttgctcc aatcactatc tttcattata
    84001 caacttattt tgaagcattt acgactactg attatctaat tcgtgtttct gatatacttg
    84061 tcacaaagcc ttccgaactt gccttctacc caattccaaa attatttatt cgaagagtag
    84121 gagaccatga ggcagcatcc gcttttagat ctatggaatt aggagaaggc acaacagaat
    84181 gtagagaacc agaacatgcc tttgaaatgg taaaactact cacagagcaa agttccattc
    84241 tacagcgaat gaatgaatgc gtcatccgca atgcaaaaga cgggatctac gatggcgcta
    84301 aaaatgcagt tgaaatggca atgaaatcac tttaaatttg ccaatttaca ttctgaaacg
    84361 gtaaatcgaa agtaagagaa aaaattatct tctattaatt tctttctaaa ctttcggttt
    84421 ccgaaaatgt ataaattatc ctttgctctg ctgagtgcaa catataggat tcttctttct
    84481 tcttctatta tatcaggctc ttcctcaatt tttaaattcc aaccactgtg taagtctaca
    84541 aagacagttt taaattctaa acctttcgaa gcatgaatcg tcattatatt tttctcagga
    84601 actcctagat tgatcaaagc ctgaattcga taattcgacc tacaaagaac cattaaatca
    84661 gagtcgatag ccgctttctc tcgaatgttt ctacaaagag attcaagact ttgctcttca
    84721 tcaatataaa tcttataaac ctttgccttg ccctctctaa aaggtcttac ttttttcttg
    84781 atattctttt tattttttcc aataggaata gcagaagttt cgattacttc tggaagagat
    84841 cgatagtttg ttgtgagaaa atgaattgag caaggattaa aaaaatcttt gaactttaaa
    84901 aaaggttcgg tgcttgctcc tctaaatcca tagatcgcct gccaatcatc ccccacaaca
    84961 caaagcttat caggactcag caaccgtagc cactcaagct gagttaaatc tgtgtcttga
    85021 aattcatcta caatgacacg actcacttct tgccttgcaa gtattgccca gtcttctttt
    85081 tttctaagtc catttaaata tatttcgacc aaatcatcaa aatctaattt attcttttct
    85141 tttttatata gcgcatacgc agtgttcact tctattgcta gacttggaaa atttttttct
    85201 agaaaatttc cagtatttga aagaaggaaa tcataaggga ttccacccac tgtaaacctt
    85261 tctcttttaa aatattcttt tagaatgctt tctttttcat ttggttctat gatttgaata
    85321 ttcacatctt taaactgtgg atgatatttt tgcagaatat aaagacagta tgcgtgaaag
    85381 gtttgaattc gaatttcttg atgacctact cttttttcaa gtctttcctt tatttcgcct
    85441 accgcttttc tagagaaggt aattacgagt atccgcatag gctcttcttt cttctctaaa
    85501 ataattttct cgagtaacgc aatcatggtg ctagtttttc cagatcccgc tgctgcaata
    85561 acctgtttaa aattagattc atcctctaat attttttctt gctctaagct gtatttcata
    85621 tttgtctcct agattgtgaa cttgggattc aaatctgttt cggaataatt ttctaaaaca
    85681 gaggaatcta tttactaaga gaattttttt ctgaggagtg tgcgtttttt tttctaaaac
    85741 ttacgcctta gcgcaaaaaa gatgagtatg aataaaagtt cagagaattt taaaatgacc
    85801 atggttggct ctttgctggt aattctaaaa ttagaaccat ttgcccaagt gacacaaaaa
    85861 aagagttgtt aaagtctatt ttttcttcgt gtcacttcgt ggccttcggg gtaaaattaa
    85921 ttttatagaa actctaaatt aacctgactt cgatataaga aatttcatca attgatgaat
    85981 acggatagtt ttaaattttc tcattttttt cgatatcctc tgtggcaatt tctaaggatt
    86041 aatacaaaat gattgagagc aagacggagc gcttgacgtt ttaaaagcaa gagaagcatt
    86101 tgtagaaaga ttattcataa aagttgcatc tgattgatca tattctgcaa ccgctacatt
    86161 gtctattccc aactcattta atgctgcacc tgttctagaa aatgcgttat ccacatctgt
    86221 tgttcctgga tatccagaag ggattgtaga agaagttgct ccatcggata caaatccatt
    86281 ttgatcaaag agtccgtaat tatttggaag tgcagaggaa acagagcctg caagtccgca
    86341 aaaagtgctc acatttttaa taggagttgt attggttaat cctgattttg cgacagtata
    86401 aaagggtgct gttgtttttt gcacataggc tagtaaatag tagatgtttg tattggaacc
    86461 acagagagaa ttcaactggc cattgagacc tgagctattt gtattcatcg ttacaacagt
    86521 tctaacacaa atatcagcac cattgttcat ttctcttata atcactctac catttctcga
    86581 aatccaagat gaatcttggg gtccattgtt ccaactatag gtttgtttca tatctccctt
    86641 atttccagat gtatagcttt ctactatcgc agaagtcgcc gttccaaaag cagtcggatt
    86701 gagtttagaa agattgtagt agagaagaac accagtcgtt cctgtccccg taagactaga
    86761 aaaaaacatt tgcaaagttg gaacactatc tcctgacttc caagcttcaa atcgatgcgc
    86821 gtaagtctta gttccactat aagcagtaga cgctatgcca gaaacattct cattgatttt
    86881 caatctatag gtaaatgtca ctcccgtaga agaagtctga tttggaacaa tgagttcacc
    86941 actggaaatt ctactaaaaa caccagaagt ctgcaaacta gttagaagtg tatcaacaag
    87001 agtagaattt gcccttcccc aagttgccga ctgacgcaca aatccccaag acgcagctgt
    87061 tcctgcaaaa gcctgttgca ctccagttaa ttctaaatta atttttccga aagataataa
    87121 agaatccttc tgatttgatt ttctctcact tacattaaaa gaaattccaa gaaaaacgat
    87181 caatgcaatc cataacttga tttgacttct tctcattctg ctatcccctt tggaatgcat
    87241 acaatcattc cggatgtgca actacttgat gaactagtag tagattggtt tcgaattgtt
    87301 ccgagtaaaa ggagatttct aattgagtga tcctccttct ctttctgaca agcatataga
    87361 cccgataaaa ttaaaactaa gacgatcaaa gtttttttta gattcatatt ttccctccga
    87421 aaaaactgtt cccacaaaaa atggttttgt caagaaaaga atcatattta ctttttgaat
    87481 tgtataagag atgaatttct attttcatga aaatctatcc ttaggatttt ttagaagcta
    87541 taaaaaaagc tataaatcag aaatgaaatt gaattaaata agagaataag caatgttaac
    87601 cttgttaaat tatatatgaa atcgaagaaa gaaattgctt ttcacacatt ctatctgcta
    87661 gtgattggaa ttagttttat aatcttgaaa cttttctata aaatcgcaga caattctgat
    87721 ttattatttc tactcaaacc aattagtttc attatagatg tttttacaaa ttcagattcc
    87781 tttttttcat tggaatctgg atattattac ccagatctgc aaataacgat agataaatct
    87841 tgttctggat ttcaattttg gcttctaact tctacttttt ttggatttca attcttttgg
    87901 aaaagaacga gtaattcctt tttgattctc ccaatgctca tattagctac ttatttttta
    87961 acgataatcg caaatatatc tagaatatta attactattc agactagaat tatgcttaaa
    88021 tctttttcca ttccctttac ttcaaatctt catgaaggat tgggtgcttt tattttttta
    88081 ttcttcttta tatcagcaaa cctatcacta ctatatttta tgaggcaaat gaatgcgaca
    88141 ccttctaaat cctaaatcta ttttttttat cacaactctt cctgcctcta tactctcttt
    88201 attaatgtat gggaatttta ctttgatcca aactctatta gaaaagaact ctattgatct
    88261 ctggattcaa tttggaattg gactttctat tctaacgatt ctttctactc tatcagccat
    88321 tctattccta tggaggaaat tagaggctaa tatatcttat gcgctgtatt ctttgattgg
    88381 ttatacaacc cttctatact cctactttta taaatttgat tctttaattc ctatttcaat
    88441 tccccaatgg atgatcgcag atgaagtctt tttttatgca ggcacatttt taatgcctac
    88501 attacttcat tcaattttta tacttgttgg gaaactaacg gataatgaaa agcaaggtca
    88561 tgttattaaa aattttattt atgctttact tattccggct atttggtatt tattttttca
    88621 aatcattctt cccctttgga aaacagaaaa ccatgctcgt agagatcatg ttcttttcat
    88681 tttctttatt gtaggaacta tcttattttt attcttcgta actcgctgga tttatgttct
    88741 aagcttaagt aaatctcatt tcatcagaag atatgaatta atctggaaaa ttccattttc
    88801 ggttttattt ccacttcttg gtcttgcaat caataacggg tttaatccaa aatcatttga
    88861 atttaataat tatgcttctg gtatttttgg agacttcaat ggattttggt tttatgtagt
    88921 tgcgatactg aatggtcttc taatctgtct accgaattta gaaaataaat tctatcgata
    88981 ctttttgttc tttggaagaa gtttcacttt tagttatact ttttatttct ttttggtatt
    89041 tattcctttt cttccttttt ccattgttgg tgtaatttgg tttggattag gatttctact
    89101 cttgacacct ctcatattaa tgattattca ttccaatgaa ttgatttctg attttgaatt
    89161 tttgaaaaat tatttttcta aaggacagat aatctttact gtatgtttcg gaatattgtt
    89221 attacccaca tttatacatg ctaactttta tttagataaa ctcaatttaa aatctgcact
    89281 ctcttattta tattctccca attatttaaa agaatacaat ttaaatataa aatcaattta
    89341 ccagaccatc gaaaaaataa aaattcacaa agaaagaaat agaggctttt tcagctttgc
    89401 aaacaacaaa actccttata tctcttccta ttttagatgg attgtattag ataatctaac
    89461 tctttctgat agtaagatcc aatttatgga gaaagttcta ctcggagtga atgatacgat
    89521 agatggctct tcaatagtag aaagaataga accaaattta atttcgaata acgttattat
    89581 ttccaaagtc aaaactaaaa gtgagtataa tccagataaa caattttgga ttagcaaaat
    89641 cgacttagaa attaaaagta attcaacctt ctttgaggat gagtattcta cttccttttc
    89701 tctaccgatt ggaagctgga ttagtgatta ttatcttttt gtggatggta aaaaagaaaa
    89761 aggaatactt gcagagaaaa aagcagccac ttggatttac tctcaaattc gccaagtaag
    89821 gaaagatcca ggattgcttt tttacgaaaa ttctggcaga attcaattta gagtttttcc
    89881 attttctcgt ggacagacaa gaaaaactgg atttgagatc attcataaaa taccaattga
    89941 gttcaaacta gatggaaata cgatttcttt aggtaaagaa aaggatataa acatatattc
    90001 cgatgataag ataattttta ttccaaatgc aataaaacaa aaattaccca ctaaacgaag
    90061 aaatccctac tatcattttt taatcgacac atccatttct aatgaaagcg actttgaagt
    90121 tctggctcaa agaattgaaa cttttttaag cggaaaacaa gaattattct cacctaaatt
    90181 ttcatctgtc aatctttaca caacgagtct tgatccaaaa gattggaaga gtcagctaaa
    90241 aaaaataaaa cgaaatggtg gctttttttt agaaagagca ctacgaaaat ctctttttga
    90301 aaattatatt gcgaataaaa atgaatttcc aatctttatc gtagttaaaa atacaaatac
    90361 aaaaactata ttcgaaaaca atttatctaa tttacaattt acatttccag agacagacac
    90421 ttactatgaa ttattagaag atggaaaatt atattccaat tcattcttaa atattgctga
    90481 acgtggtatg cataatagaa ataagctaaa tatagatttt actgtatatg aatatagctc
    90541 agatgggatt acttattctt taccacaaag agatagctca gaaattattc taaaaaaacc
    90601 ttcttttcca ctttccgatt tggatattaa agaaaaaact tggagcagtg gactttggct
    90661 agaagccaaa tggatgtctc accaattatt tccagaagat tctgaacggg aatggattga
    90721 acttgtaaaa tatagcttta tttcgcaaat catgacgcac tcaacatcct atatagtcct
    90781 agaaaatgaa gcacagaaag aagcacttag aagaaaacaa gcagctattc tttccgccaa
    90841 taaatcgtta gacgctggag aagaaacact cagaatgtct gaaccagatt tctatctact
    90901 ctttctatta ttcctaggat acttatttct taaatatttt tttaaaagaa ggcaatttga
    90961 atgaacccga gtgatttact attaattgga ttcctatttc tagtcggggt tatttttgga
    91021 tttatttcga gtgtttttac aggagttttt aatcacttct taatcaatag attcttaaca
    91081 gaatctattt tgagaaatgg aattaacgta tttttaatat ccatttcttc cttcctcggc
    91141 tccatgtttg gattcattgg agtaatcgga tttgctgcgg atgaaaatgc tggagaaatc
    91201 tttccgtatt attttgggat ttcgattcta gtctctatac tctgttccat tggctattac
    91261 ctaattagaa ttcgttagaa tagccaattt ctgaaaccac agtgatttga gacttttacg
    91321 ctaggaggct ctatttgcaa attagaaaat atttctgaac aaacaacaca gtcttttaat
    91381 cgatctctta atttatgctt ttgattcgag tcaatacttg accaaagatt ttttctttcc
    91441 gttgaatctt ctttgatatt atagagttcc tcttgcttag gaattgaatt tatctttgct
    91501 atataatgta aattgttttc tataaaacta atacccactc tatagagaga aatttcatta
    91561 ttatttgaaa tcactatgac tctatcagga tcaatagaat ccattaatga tttaccaaca
    91621 aagctgacag tgtgaaaaga gtctaaactt ttatttaata aatgaacgat agttggtata
    91681 atatctaaat tcatcacatt cttattcgca ttggattgaa gattgtgaaa tgtatcatag
    91741 gtttgtagtt tcttaggaaa ataaaccatc attggaatag aaacagtttc tagataataa
    91801 ctctcaatat gacccaaata attgtgttca agaagcgatt caccatgatc ggatgttata
    91861 atcacaattg tattctctaa attattcgta tcctttaaag tttgaaacat tgattcaatt
    91921 aatttatcga aatgataaac cgaattatca tacatatcct ttacatcatt accctgccat
    91981 actttcgagg atgaaggaac aaaaaatgga aaatgatttg tatttaaatg gagtaatcct
    92041 gaaaagtttt tattttcttc tgagagttta cgaatatgct tatcaaaagc ggctaatgtt
    92101 tttctatcat caatacctat atcattaaaa attggttcat tactattttc cctattgaat
    92161 tgatagtcta tacccccatt tgcaaaataa ccacctagat tattccatgc aaggctatgc
    92221 gatgtaatat aaaatgtttt cttcccagac tccttaccat actcccaaaa taaaggataa
    92281 gaatgtgtat ccgcagcact ctgaatcgga gatactcctg ttaaaatact cggtagcgaa
    92341 atcaaagtag aagaagcatt cgagtatgca ttttgaaaaa caacaaattc ttggttcttc
    92401 tcttttgcat atttcttaag aaagggagta gtatctcgag agtatccata cagacttaga
    92461 tgatctcttc ttaaactctc agcaactact aaaatcaaat tcattttgga atttggaaac
    92521 ttttctttaa ttgaaatttt agttctagat tgtaatcctg ttgaacccat ttgatttgaa
    92581 attacacgat ttgcaatatt tcttgatata aatgcgatag tgtttatatc tgatacataa
    92641 agttgatcct gaaatctagt gttaaaagaa ataattgtga ttaaagaaag aacaatgatc
    92701 ccaagaattg aaataaaaat tttacctagt ctattgaatg tctcagttac cctagaaaat
    92761 actgaaagaa gagcaacact gaaaataaag agtcctagaa atgtaattac actaccaaaa
    92821 gtaaatgaat cttttaaaag tgtaaatgaa ttgagaggtt cctgaatcat ataatgaata
    92881 acaaaataat ttggaagcat gttagctttt tgataatagc cataactacc tacgattgaa
    92941 aatgtataaa taaatcctat gactgtatac aatagatatg atatccaagt tgatatccgt
    93001 tttgatattt ggcttgtgaa taaaaagaga aatatataaa gtaagaaaga agccaatatg
    93061 gataataaat agtatcctct cattttctca ttgaattgaa gaatcgtatc gattctaaaa
    93121 tagaaatcaa ctgacaaaac aagaagaggt aaaaagagaa tgagtattaa tttttttaaa
    93181 actttcataa tggatacaca ggaaacaatt tttattattt ttgtttccta tactttttca
    93241 acgtttttgg ttgtcaaatt gaaagttatt tttttaaaaa agagataaaa ataggtagtt
    93301 taaacaggaa gagtttagta aaattgcctt tattttttag ttttctgcga gattgattct
    93361 tgatttaaaa ttccaaaaaa taaaattgta tcaatttctt ctatcagatc agcaacccca
    93421 acacctaact ccaagagaac ttcaggattc atcaattcat ttacaatacc tagatacata
    93481 cgaataaaaa catctgtgtt tagttttttc tttacaatcc cctgtttcat tccatgcttt
    93541 agaaaactac ccattcgaat cggaatatcc ttatttcgca ttttcacaag ttcttgccaa
    93601 acctttggtg catttgtttt cacatcatgc ataaaatgca ctctctctct cggattctct
    93661 ttacttatat attctatcat ctttctgaat ttggacataa aatccaaatc attgtcttga
    93721 atgataatat cgagtgctgc ccctttacaa tgagatcgat tgactagaac ttctctcagc
    93781 aaatcttctt tgttagagaa atatttatac atagtttttc tactcataac taaggaacga
    93841 catatttcat ccattgtaac tctggaataa ccttagtcta aaaataactc ttccgctttt
    93901 tctaaaatac ggtttttagt tttattttct tctgataaag atattcgctc aggctgtttg
    93961 tttcttttgg tcatatttaa aacgttatgt agaatttttt cctcataata agcttatgta
    94021 tttttctggc aagtaaaaag attcagtttc ctcacaatat aaatatgaat aaatttgata
    94081 ggaacttaat ctagaagata gatattaaca taccctatat atttcgtgca taatgaatta
    94141 tttgtctaga gacaataaca ccgaaaagag taaatttact aaactgagat atcgccttta
    94201 cagatctttt atttttaatt gataattata tagttttatt cattttgtgt tcgatgattc
    94261 gagtttccat ttttatttcg atattatttt ttcttcattg cgcagataac agaagttata
    94321 cggaagctag aaaaatagcc atcacacaat atacactctc ttgcacggga ggatcttaca
    94381 actcttgtag aatgtcttgt gacagtaaat gtggaattga ctcgaatcca atcaacagtg
    94441 taaaattaga ttgtgctaag aattgcttag acgattgcaa ccgaaattgc aacaccatac
    94501 tcttatttct tctcaataca aaaaatgatt aatttaaatt gtaacaaatg aagacctttt
    94561 cttctctaaa atccattcta tttctaagtc tattttttta tacatctgtg agtatttttg
    94621 cagaagagga tacacagata tatacgcaaa aaccaattta ttcttttcca atactagatt
    94681 caccatttaa tactcaaaga ggttccctac aaacagagaa taattctccc gtccctagta
    94741 tggaacaatc tattcatata actaagaata taacacaatt atctcataga gcacttgatt
    94801 caaaattgag tgaatcagaa actataaaca aaaacccaat tttaaaaaga ctaggaattc
    94861 tcacctttga ctttttattt tattcaattc ctattccagg aaatatttct tggatgcatg
    94921 aagaatggca tagagcagta atgggaagaa gaggaatcgc ttccttcaat gatgtctatg
    94981 tagcaagacc attctcggaa tctataagcg ttagtcatgt taaggactcc gatttaattc
    95041 aattcaagaa agaccatcca gaagaattcg ttcgactttc agcagccgga atggagtcac
    95101 aaaccgaaat gaacttacgt atgcgtaagg atactttctt tgggggaaca gcctcctatt
    95161 tagatacagc tacactttgg atcaatacca caaataacat tggttatctt cagacttgtt
    95221 ccaaccgaag tgccgataaa tttacaataa aatcaaacct agaagaaaga aatatctcta
    95281 aaagagattt cgtaggactt gattgcaatg cttgggttta tgatttgttt agaccaaatg
    95341 aaccttatga actgagggga atacatccaa caggaattgg aattgatcgt tatataaaat
    95401 acagcaatga aacaaaccta aacacaagat ttcaaaattt tcttttgtat ggaaacggca
    95461 atcatcattc cgatttaaca aaacaagaac aggattatat aaaccagcag tataaattga
    95521 gttatttaaa tttaatcaac cctgccctca taggactatc tcgatttacc gcaataaatc
    95581 catttaatgg taaggaattt ttttggagtt ttggagttgc tcattatcta acgtcatttg
    95641 gatatacgat agatcttcag tttctattca aacaagagaa gctaaatata ctctttattt
    95701 atcatactta tgcaaatgca tcgttgaaac ttccagggat tgaagcggaa attatccgtt
    95761 atccgcttgt tgtatttggc aaagaaactt acataaattt ggggatgatt ttgtggctac
    95821 aaccctacca acagaaattt tggacgacag agaaagaaac aggtggaatt ataaaagcta
    95881 ctctgtcttt gcctctattt gaaaacttag aagttttttt ctcgccctca ctaaaatcac
    95941 gtggttgggt tgcgggcaac gtatatttag ataaaatgtt cgaatttcgt tcaggcgtaa
    96001 attttatatt ttaatatagc aaagcgaata tagatttaca ccaatataag ttgacttctg
    96061 taaaattaag tattttccaa atcactcggc agcttaccag acttgccagg cagctttcca
    96121 gacttgccgt gccttcttct gtaagtccaa tataaataaa aaattaaacc agttacagta
    96181 atattcacaa aagaaattag atggagtgca gtcgcataca aaagtccata attagaatct
    96241 tttccaatta aaataaaacc agaaataata gaggcatgaa atacaccgat tcctgaggga
    96301 gcagaaggaa gcgcaagact catagctcca caaaataaca aaaacataat ttcagcataa
    96361 gtcagttcac cacctaacat cacattaacg tagtagtagt tcgcaaagta gaagcaccaa
    96421 gaaattattg taaatatcat tggataaata aacttcttaa cttgtaagaa atcttttaaa
    96481 tcagctagat gaacctctat atgatctttg tagaaagttt ctttgcctac tagcttaaaa
    96541 agttttgtgg aaacaatctg aataaattca ttttgaaatc gaattaaata caaagagaaa
    96601 agaattccta gaactataac cccagacact gtaaatacag cataactttg ttgctctggt
    96661 cgatttaagc caataataaa aaaagcgata actcctgcga gtataacaaa aataaaatcg
    96721 attacttttt cgataaaaag ttttgataat aaatgaaagt attgaacatc tgtctctgag
    96781 cgacagtaga aaactcgaaa caaatcacca ccacgtgcag gtaaaaccat attagccgca
    96841 tatccaataa atgaggatga aatggcatgt tttaaattga ttttcttctc gatcaatagt
    96901 ctccagcgaa aagaaaaaat cactaatcca atcagctgtg tgactgtgat agggattaag
    96961 tatgcgatag aaatcttatc tccaaactta gaaaattctt tctgaaaatc atactttgtt
    97021 ccaataaagt agataaagat aatacttaca aaaatcccga tgaataattt cttcatgttt
    97081 gataagtttt caaaactagc ttttgaggca agtaagtaat taacccggtg gccattccat
    97141 tttacgtcca gacaatagat ggaaatggat atgaaataca gtttgccctg catcagatcc
    97201 tgtattattt acaatacggt agccaccatt gttaaaattg tttttacgag catattccgt
    97261 catttccaga atgatatttc caattaatgg tgcatctgct ttcgacattt tgtctaccga
    97321 ctcaatatgc tttcttggaa taaagagtat atgatttggt gctacagggt taatatctgt
    97381 aaaagcgata caattctcac tttcatatac aatattcgcc ggaatcattt tatctataat
    97441 tttgcaaaaa atacaaacgc tcaaaactcc tccctttact caaaagcgag agcagcagcc
    97501 ccatagctac ctgctaaact acttcctact ttgaattcta tataattgga taatactgga
    97561 aacataattt gctttgcttt ttcttcaagg acttttccaa ataaagaata agaagctgta
    97621 atcccaccca cgaatacaat tctatcgggg tttaccaaat gaacaacatt tcggattccc
    97681 tctgcaaaag cagtaactcc aaagtccaga acttccatag cctctttttc tttggctgct
    97741 actttttcaa aaaaaacttt tgagtttgga atttcaatgg atgttctatc tttataacga
    97801 ccaataaaac cttttgtgct aaaataagct tctacgcatc ctcgctgtcc acaaccacag
    97861 agtgctccat tttgcacaac cgttatgtgt cctgcctcca ttccatttcc tttaaaaccg
    97921 ttaaagactt ttccatttaa aacccatccg caaccaagtc ccgttccaag agtaaaaaca
    97981 ataagactct tagaaccttt gcccgcccca aaatagtatt ccccaaggct tgcacaattt
    98041 gcatcattat taaagaaaac gggtaaatga aattctttgc ggataaagct tgttagctcc
    98101 acattagata aatttttcaa attagctgaa gatattatta ctccagagtc tatatcaatc
    98161 ggaccaggtg ttccaattcc aattccattc acttcatagg ctgaaataca tttttttaca
    98221 actgtctgaa ctgattctaa aaattcttga ttataagaat cgggatttgt tggaacacta
    98281 aaatcaaaat aagaattacc ttctttatca acgacagttc ctcgaatact ccctccgcct
    98341 acatcaatac ctaaatattt tttcattggt aaatccttat tagccgctta cattcagagt
    98401 aattcgttct ataatttttt gtttttctac ttcagaaaat tcatgcccat agaatcttag
    98461 gaacatttgt attttctttt ctttttctga aagatttttt ggaagagaat actccacaat
    98521 tttttttgct gcatcaaaca tagaactacc gatctgaact ctctcccatc ccgacaggct
    98581 catatatttt tttcttagca tagactctat ttctggagaa gtatcattca ttcattattt
    98641 cctcatacca atccttaaga tttaactcat ttagtttttg ctctaaataa ttcaaatcta
    98701 cttctgactt aactagattt tgaatatctc tcttttgcat ttctgatttg gattttttag
    98761 accagagtaa cttagataaa atcaaatctt ctttactcac tataaaagtt tgaaagtctc
    98821 caaattctat tttttttctt ctttcaaact cttctaatct ataatttcca gacttcctta
    98881 ttataaaatc aactttgata aaagattcat catgaattat attaaatagt ctttcacttt
    98941 gaattgcaat atctatagaa tctggctcca cataatagtc agccttaaat aaattaacta
    99001 tagtcgatat atcttccttt cctatctgaa ttacaatgtc aatatcccta gtcattctag
    99061 gtagactata ataattcatc gcaacggaac cggtcaacat atattgtata cttgctgaat
    99121 ctaatttttt tgaaacatct cgaactatat caatttcatt tttcattttc gaaatacata
    99181 tgttctcaaa tctctcacaa cccaaaccca tatcccgaca aacatagcag tagagagcca
    99241 gtttccaaat cttgatccag gaagtaggaa aaaatcaact actccgtgct gcatcacggc
    99301 tagaaaataa ccatagaaaa taatgataag cttatctcta aattgagttt gaaaattaga
    99361 tttcaaaaga aataaagaaa aacataaatt aatcaataaa tgaatgtttg aagaaaaaat
    99421 agttctacca ataaacatag tataaacttt agaagagtct tttccaatgt aatgataatt
    99481 ctcgatcaaa gaaaaaccaa gcgccacaaa tccaccaatc aaaacaattt ccttttgcca
    99541 ttcctttgag gaatatctaa atgctgcaat caatccaaaa agcaatataa acaattcttt
    99601 gaatgtttcc tccatcatac ccgcttgtat aaaagaaata taaacagtct gagtgaggat
    99661 tgtagcgttc ttctttggct taaaatcaat ttcaggccat ataataggat gtgcccgtag
    99721 aatcagttct gtagataaaa caccgagtaa aagagcgata aaggaaataa caatttttct
    99781 ctgcaattta gttggatgaa gtgcaaccaa aaaaaatccc catgggagta tagagaaaaa
    99841 agcaaggata gctactttgg gttcaatatt agataaaaaa tggagcattt aaaattcttc
    99901 ttccatgtat cttccatcaa aatgaaggac catgtctttt tgtaaaggtg ttggcatagc
    99961 atctggatca atcgcaccat tccaaaataa ttctgtaact cttacatcac ccttgacgtt
   100021 aggcggtggc atcaccggag acatactacg aattaattct aaagttagat tgtcttgatc
   100081 tggataggcg gaactttcta caagtattac atccactatt tccccctctg tggtaattgt
   100141 ataagctaca acacaggaat aaggatgagg agctcgtttc caatactcca taaaattttc
   100201 tggaccacgc atacgagcag aaatataatt tgaatatgtt ttgggaagtt ttttcccttt
   100261 aatacgttta acgtatcctt ttacttttcc ttcatcagtg acaacttgat ctggaatctt
   100321 gattccgtct ttttgactcg gagtatctgt tccagagatg actcctccat ttaagtcttt
   100381 cacattatca ggaatttcag gatcaataaa gtaatattct aacttttctg gttgatagtg
   100441 ttgtttggaa atatcttgtt ttttcttgga ggaagaactt ctttgtattc tttctggaac
   100501 aataaataaa attgcaatga taacaagatg agccaatata ctgagatata cgttattatt
   100561 aaatccactc tgcatccctc tttcagataa cggttcccca tcttttttga gggaagcatt
   100621 tagagtagag tttgcataga tataaatgat acttagtaga aaacttaaac caataggcaa
   100681 agctaaattt tgtgtaagcg gaataaactc cttatcatgt atcccaggtt ttaatccaag
   100741 tgatctaaga aaatttaaac caaacattgg atcaaagtaa aatgtaagag caatgactaa
   100801 aaaaaagaaa acaaggagga aacaaaaaag gattggaaat cccttccagt aacggtaaag
   100861 atagatatgc ccccagcctg gtaaaattct ctctcgaatc ttggcatatt ttttctttct
   100921 gttatagatc tcactgaaat agtcgatcgt cggaactctt gctagtctgg aatgcacata
   100981 aagacttaag aaaggtctgc gcaaaacatt gagtattccc tttctaccca gaaaacccgt
   101041 cgtggagtat aaggtcataa gccaaagaaa gattgcgact tgtccatttt gaaatagcat
   101101 tttatcaata gtggcagaaa tctcttcgct attgattgtc tctgcgttta aagccgcata
   101161 gaagtaaaaa agaaaaacaa accaaaaaac agagataacc catgtaatca gaacagagct
   101221 cagcattaca gtaaatggtt tcttcgaaat caccttcttt ggcttctcag aattttctga
   101281 ctcagatctt tctactcttt gatattcata attatcccat tcttcggatg gggttgctgg
   101341 tcgaaccacc atcggagtat caataggatt cttttctcga aataatttta atcgaccaaa
   101401 tctacggata atcagccgag cctcaataaa taaaaaaagc agtcccatga gcgcccatat
   101461 tgccacaatc acacccatat attcttgttt taaagttaaa atatacgctt ctgtgtcaaa
   101521 gttcacaagt gaatataact caatgggata ggaaatgact tggataaaaa cttgaaatgc
   101581 aagagactga aaggcagcta ctctgagttt ggtgttatgg gaatataaaa ctaaaacacc
   101641 gattggaaag aatataccca actctaagaa cggacttgcc cagcataaaa aggaaagaca
   101701 agagaaaaca aaaccattca agaaacttcg ttttttttcc tcaactggtt cttgttcata
   101761 aacaatcaca tcttgcataa gttattaatt tgacgtatgg cttactactt caagcgaaaa
   101821 agaaaaaact ttatataaga aagcttataa aaaatatgta aagaatatgg caaaagatag
   101881 agaagattca ttaaaacaaa ttgtagcagt ctctaaaaga agaggctttg tatttccagg
   101941 ttcagatatt tatggtgggt tatccaatac atttgattac ggtccctatg gaattgaagt
   102001 attaaacaat ttaaaacgac tttggtggga atacttcgtg cacagaagag aagatgtagt
   102061 tggcctagac tcttccatcc tactcaatcc gactgtatgg gaagcatcag ggcatgttgg
   102121 aaatttttct gatccactta tggattgcaa acagtgtaaa accagaattc gcgtagatca
   102181 ttttctagaa gaaaaaaaag gcgatggatt tgccagtgga aaaactttga aggacttaca
   102241 gcatgttatc ctcgaagaaa aatatccgtg ccctagttgc ggaagcgtag gaacttttac
   102301 agagcctaga agttttaatc tgatgtttaa aacctcacat ggtgcctctg aagataattc
   102361 aatggatatc tatcttcgcc ccgaaaccgc acagggaatt tttataaact tcaaaaatat
   102421 ttacacagcg actcgaaaaa aagtcccatt tggaatcgca caaatcggta agtcctttcg
   102481 aaatgaaatc atggctcgcc aatttatttt tagaaccaga gaatttgagc aaatggaaat
   102541 ggaatatttc tgtgaaccag gcacacaaac agattggttt acattttggg tagactattg
   102601 tatgaagtgg ctaacagatg ttataggtat aaaaaaagaa aacctgagag taagagaaca
   102661 tgcaaaagag gaactatcgt tttatagtga tcgcacagcg gatatagaat atcaattccc
   102721 ttttggttgg ggagagctgt ggggaatagc ctcaagaact gactatgatt taacacaaca
   102781 tgaaaagttt tccggcgttg atttaaaata ccacgaccaa gtggctaaca agaagtatct
   102841 cccttatgtt gttgaacccg ctcttggact caatcgactt ttcttagctg ttatgagtga
   102901 tgcttatgcg gaagaaaaat tagaagacaa cgaaactaga actgtgcttc gatttgtttc
   102961 aaaagtagct cctatcaaag cagctatttt tcctcttatg aaaaaagatg gtttggatgt
   103021 aaaagcaaaa gaaatttatc attctctcat gagccattgg tatgtggact atgatgactc
   103081 agcggctatc ggtaaacgct accgtcgtca tgatgaaatt ggaactcctt attgtatcac
   103141 tgtagattat gattccctga aagacaatac agtcacaatt cgagaaagag attccatgaa
   103201 gcaagaaaga attttggtag ctgatattcc aaattatctg cttgggaaat tgaaataaga
   103261 caatttttta cagagacgga agtaaaatcc tgttttaaaa aaggggaaat atgactatta
   103321 tcaaggctct aagaataata aacttgcaaa agttttataa aatgtttaga aaactattat
   103381 gttacattga attattggat ttttattctt aagccttata tctttataga aagtataaat
   103441 gatttatgta attttagata cgaatatatg gattttacca ttctaacggt tttaaatcgc
   103501 cagatgatga ctcatttaag cgtaatccac ttgaaaggga tgctcatatt gacatcacac
   103561 aagctctaat aaacaaatgc aatgataacg aaataatttt attaacaaat aagattattt
   103621 tagatgaatg gaaagcaaac aaagaaaggg cagaagaatt tataaaagaa tgtaaacaaa
   103681 cagcaaaaga gatagaaaag attcgaaacg ttattagtcc atacctatca agagatgata
   103741 taaacaagca gaaagaatta atagctacag ccttaaaaaa tataaaagaa attattgata
   103801 aaaataagca acataaagaa acgattgaaa atctaattaa gaataaatgt gagcatatag
   103861 aaatctccga tcatgtgcac aaatttgtta gcaagctaag aacagacaga gccatttcga
   103921 tttttaaaga caaaaacaaa gataattaga atgatgcgat aattcttttc agtgtaatcg
   103981 attatttaaa aggaaaaatg aatgaatttg atagggcggt attcgtttcg aataattaca
   104041 aagaatttgg taaagacagt aaagaagatg gatttcattc tgatttaact caatattttg
   104101 aaaatgtaga aattatctac gaacgcgact tcaagaattt attagactta accgaagaca
   104161 tggaatatga attacaaatg tattttgact atttacatga aaataaaatt ggctttcact
   104221 gtcgaatgga agactgtaat tcaatgggta ttttcgattc aaaaattcga gtttctgatg
   104281 gtagtctcga atatgaagat cgaatgcagc taaagttttt tcaactatcc gaagttccgt
   104341 attctccaaa agttaaattt gtccaatctg gaagatgcaa gaagtgcaat tctattcata
   104401 ttgaatgtcc agagtgtaaa aatctcctga tagattttga agaagataat atcctatttt
   104461 gtgataattg ccaaatcaga tttgaaagag cttactcaaa tagaaataat gatgaaatta
   104521 ttataccaat taatgatgat atattataga gcaactgata catctaagaa taaaataaat
   104581 taaccagaat tagagctaag aattgttccc tagtgaggaa cacagatcgc ttcactaaca
   104641 ttggtgattt taaattcctt tatttgtatc ggtgtcctcg gtgcttatcg gtggtaatct
   104701 tttaaatttt ctattacatc aaactggcat taagactatc gtatttctaa atatctttta
   104761 ggcgaagtcc cgttaggcga ctgatttctt ttttcttata gccggctgct aaaagttttt
   104821 ccaccacaag ttttattccc tctttcctgc cctcgcgcct tcctttttct aagccttttt
   104881 ctaagccttc ttcaatacct ttttcaactc cctgcgcccg ccctttttcc atcgcttctt
   104941 tttctgcctc ttgcactcga aacgccagca tcgaaccata atctttttct gctttctctt
   105001 tcatctcata gtattcacgg gattttttat ttaaggaaac aaatcttaac tcttgaatgg
   105061 ctttcttaat ttttggattt ttcttttgaa gtgctttcat agtttctcct tttaaagatg
   105121 atgcttcttt taataagtag acccaatatt ccaatggagt ttttaattct gcaaactctc
   105181 ctatcaactt tggtatctct ataatccgaa tacttaaatc ctctgtcaat agaaattgcg
   105241 gatcatcctt ttcaatgagt tgaaaatgcc aatagaactt agatttattt ttaaacattt
   105301 caaaatttac aaaattgaaa gaatagactt tcggcaacct tatatacagt tcccctttcg
   105361 aaatagattt agaataaagc ttagaccaat aataaagaat cctttttgaa aaatttaatc
   105421 gatctgttgc ttgcatttca attagaaaat gattcccttt tgtatccatt gctttcatgt
   105481 cgagcgttac actcttttca aattttccat ctctaggtat ttctggattc aaaatttgaa
   105541 tcttctctat ccttccttct ccaatgaact caggaaatga attgattaaa ttaagaatta
   105601 catcttcatg ctttccaaaa acggatttga aaatgtaatc atttgttagc ggtaataatt
   105661 ttattctcat acaaaataaa gagcaaaatt ttctaaattt gagcagattt atttttaaaa
   105721 tttacacagt tacgcaaaaa aaatatattg aatcaattga tctaaagtga gttcgatata
   105781 gcaaaaaata taaaagatta ccaccgactt gcactgagcc tgtcgaacgt aaagacggat
   105841 gacaccgata aaaatgaaga atttaaaatc atccgtgtct atccgtggtt aaatattttt
   105901 acatcgaatt cacattaatc taagtatttt caaaatacta aaacaaaggg atttatttat
   105961 gacaaaagaa tttgaaggaa aagtagcaat tgtaacagga acagcttccc cccgcggact
   106021 cggtcgctct atatgcaatc gcatcgcaga agagggtgga aatttagtaa tggtagatct
   106081 taagcaagat gcttgcgatg ctgccgcgaa agaagtttct gataaatatg gagtaaaaac
   106141 aattggcgtt gcgtgtaacg ttacaaaatt agctgattgc gaaaatgtaa tcacaaaaac
   106201 aaaagagact ttcggaaaac tggattttct aatcaacaat gccggcgtat taaaagacaa
   106261 tctactgatg agaatgtcag aggaagattt tgacttcgta gttgatgtaa atttgaaagg
   106321 agtctttcta atgacgaaag ccgcttctaa attaatccta aaagctgaat caggaagaat
   106381 tgtaaatatt tcttctattt ccggtcttgt aggtcaagca gcgcaggcaa actattcgtc
   106441 ttctaaagcg ggtgtcatcg cattaacaaa agtagccgca agagaatttt ccggtagagg
   106501 tgttttagtc aatgcagtct gcccaggata tgtgcaaact gatatgactg gttctcttcc
   106561 agaagacatt caagcaaaac tcaaagaaat ggttccactc aaacgtcctg ctacaccgga
   106621 agacattgcg ggtgccgttc gtttcttttt aagtaaggat tcatcgtata tcactggcac
   106681 aatcctccgc gttgacggcg gcggtgcaat tggaatgtaa aaattttaaa agattaccac
   106741 caataaacac cgagaacatc gatagaaatt acgatagctt aaatagtcga aatttgactt
   106801 catttcaaat tgtttttatc gttcctttat cagtgtccat cggtggtgaa aaatattagg
   106861 agaatttatg agagaagtat caattattgg agcctatcaa acaaagcatg caaaccatgc
   106921 ggacaaacat ttgcgtgaac ttgttacaga agcgggtaac ggtgcaatta aagacgccgg
   106981 aatcgacaga aaagagatac aagcagttta tgttggaaat tacgcaggaa atgagtttaa
   107041 caaccaaaac acaatggcgt cttatgtatc gacaatcctt ggtattggaa atgttccttc
   107101 gtatagacta gaaggtgctt gtgcgtcagg cggtttagct ttgaaccaag gatttcatgc
   107161 agtagcaagt ggaagatttg acactgtgct tgtgcttggt gtagaaaaaa tgaatacaca
   107221 agatccaaca aagactatgg aaattgtaag taaaggtcag gactttgaat ttgaagggaa
   107281 attttgtgta tcaggtcctt ctgggtttgc attaaacgct atgcgtcata tgcatgaatt
   107341 tggaacgaca aaagaaatgc tttctacagt tgcggaaaaa aactattttc atggaagcaa
   107401 aaatccttac gcgcataaac aaaaagttgt atctttaaaa aatatcatga atgctcgcat
   107461 ggtaacaact ccgctcggat ttcatgatat gtcacttgtt acagatgctg ctgcttgtgt
   107521 tgtgatcaca acaagagaaa aggcaaaatc tttaaccaag aaaccagtca gtataaaagc
   107581 ttctgcagta ggtggagatt attttacctt gtctatgaaa aaagattctg ttagttttcc
   107641 tgcgactgta aaggcagcag aagaagcctt taaaatgtct ggtttaaaaa gagaagacat
   107701 tgatgtattg gaatgccatg actgttttac aattactgaa attatcaaca tagaagattt
   107761 aggttttgta gaaaaaggaa aaggtggatt ttatacgtta gacggtcata ctcggcttgg
   107821 aggaaaactt cccgtaaaca catcaggagg gctaaaggca aaaggtcatc cggtaggcgc
   107881 aacaggagtt gggcaggtgg tagaaatgac atttcaactc cgagaagaat ccggagaaag
   107941 acaagtcaag aatgctcgca cagcactcac tcatgtatta ggtggacccg gagcagtagc
   108001 agccattcat attttacaaa aggattttac ataatgaata cactcaaagg aaaaaaatgt
   108061 aactcttgca actttctaat gctagaaccg tcttttgcat gtcctagctg cgggagcgat
   108121 tctctatcgg atatagaatt caatggagag ggaagcatct atacttttac agttgtaaat
   108181 gtaggatttg gacatcttgc agcaaaggct ccttatgttt tagcgatagt cgaactcaaa
   108241 gagggtctaa aagttttaac aatcgtcgaa gatgcaaatc tagaaaatgt taaaattgga
   108301 gacaaagtaa aattcaaaca tatggatgaa agacaagaac caatctttca ggcagcatag
   108361 ttaatttgaa tagcgaacac agcgaacatg tttatataca tttatataac taaggctgtg
   108421 ttccccataa attatattaa agcagtaagc atcatcaaaa ctacgagctg cttcagaata
   108481 cgtccaataa gaatctcctt tttcttccca cgattttgat aatctagaat tgtatgcatt
   108541 tctgaattcc caaattgtag gaagcctcat cccaatactt tcacattttc cagccgcatc
   108601 tctccatgaa tgctttcctt ggtaatcact ccattctaat gtcttatcaa ttgagaacaa
   108661 atctgaattt acaaaagata agaaaagaaa ggggataatt actatgattc gcttcaattt
   108721 ttcaattccg attatttata aaagtatttt gatttattag atcacaagaa agattacttg
   108781 ataccaattc ttttttcgat attcgcttca ctctcttcca tcacagattg cgcttcccca
   108841 ttgatccatc gtttttcaac attccagtaa ttgggtgatt ttttcttttc taaaacagaa
   108901 ttggcagcca tggtaattaa aatagattgc gttttatgag tgaaggcatc ttttttagaa
   108961 acggcaccaa aatcaattat agtatctagt gggttatcag aactatccat ttcatacggc
   109021 tcatagtatt ttaaatcatc tggattcaga gacaatatct tatcagcttt tttcttatcg
   109081 gataccttta tgaaatttaa ctttctctcg atagtcgcat acaagatata atcctcatct
   109141 gtatcgatag cggttaatac gtattggcta taatttttta aaccatattt ttttaatatt
   109201 ttctggaaat gctcggttgc aactttcata tctttaaata cttgtccagt atcctcatca
   109261 gattttgctt cctttaattt tttatcgtaa gcatactcat aagcaacttc cagagagact
   109321 ttaagctttg ttttattgag gatgtcattg attgttgaaa tttttgtttt cgacaaataa
   109381 ggtataaacg aaattccttc gacaaatccc tttgttaatt gagcgggaga tagttcgact
   109441 gttccctttg cacttgccct tgttcctttc ggcttatcag tctttgccgc atctttgctt
   109501 gaacaagaaa atataaaaat aataatgatt aataataaag ctttatttgc tttcacttga
   109561 agactcccaa agtaaagata gatatctacg atttcttcat gctaagccat tacgaatctt
   109621 tggcaatcct atttaacgcc atttcgatat aacagaaaaa ttaaaagatt accaccaatt
   109681 gccgcgaaca agcggtaatg gattttcggc ggaaagacga ttatcaccga taaaagatac
   109741 gataattcaa aattatcaat cgtgtcttca tcgttcagtt atcgctggta attattaaga
   109801 cagcattgag cgaagggtga aatgggcacc gatataaatg aataaattaa aaaccatcgg
   109861 tggtgaaaat tcttatacca aactcacgtt agttaagtga gggatcctta gtatgttctg
   109921 caaagtagcg gtaaattccg cctttgcttc ggagagcatc ccgccataca gtttctggat
   109981 cggggtgaaa tatattaact ggacttgcgt catgcgaaac ccatgactta cgtgcaaact
   110041 ctgattcgag ttgtcctgct ccccaaccag agtaaccatg aaatacatga tatgcagtat
   110101 catctgagcc taaaagtttt aacaaaagat catagcttct acttaaaaac acacctggaa
   110161 tgacttgcaa tccaggatca tggatatttg gattattatg aagaatgcta ataaacccat
   110221 gatcaacagg tcctccccaa tacataaaag tatcttttat tccatcagga agacccatca
   110281 caacttcaga catgagatag gaggattttt tatttataac taaaccaaag gcacctgtcg
   110341 catcatgatc taccataaat acgacagatt tattaaagtc atctgtgacg atgcttgaat
   110401 tggaaattaa aatcttgcct ttgtaagaaa cgtcagtcat ttactttgaa gaaatttctt
   110461 ttaggatctg gtgagcaatt tctaaagtgc gaatgtcttt ctctccttct acttgtggag
   110521 aatcttttcc taagatacat ttcacaaagt gctcatgttc ttgttttaat gggttatctt
   110581 tgtgaacaaa gattttctct acgattgatt cttgcttata gcgaatagat tctgcactga
   110641 gttgtgtatt agaagaagcc tggcgatgca attcaatttc ctgatcacca aaatctaact
   110701 tgatataagc attcttatct gtaatattta atgttctaat ttttgcttgc gtattgcgtg
   110761 aagctgcaat agtcgccacg caaccattgg caaattttaa aacgacagtt gccacatctt
   110821 catgttcaga tacaattttc tgtccaactg catgaacttt tacaacatcc gaatcaacta
   110881 gatttaagac aatgtcaata tcatgaatca tcatatctag aacaactccg acatccttga
   110941 ttcttgcatt gtaaggagca agtcttctag attcaattag cattggagta tctacaattt
   111001 tgcctagttc taagatagct ccattgaatc tttccacatg tccaacttga aatataagtt
   111061 tgttttgttc tgctaatttt acaagttctc tggcttgttc cactgattca gcaattggct
   111121 tttcgactaa cacgtgtttg ccagctgtaa gagccagttt agaaagctta tgatgtaaaa
   111181 aagtaggagc agcaattaca actgcatcta ctttttctat taatgcttct ggtgtagcaa
   111241 aggaaggaac agagtatttt tcagaaataa cttttgctct ctctggatct gcatcataga
   111301 ttccggttaa cgtagaatct tgcacttgtt ttgcaacatt cacatggtat tgtcccatgt
   111361 gcccaacgcc aataatacct atttttaatt tatcactcat ttaaatatct ttcctttgat
   111421 ataacttcca aaagattcat ttgactcttt ggcaaattct tctagtaatt ctctttgttt
   111481 tttggttaac ttctttggga cttccacttt aataatgaca tgttgatccc ccttcccata
   111541 agaacctaag taaggaattc cacttccctt taaacggaaa acttgaccag gatcagttcc
   111601 ttccggaatt ttcatcatga tactctttcc ttcgatagtt ggaacttcca cttctccacc
   111661 aagacaagcc ataacaagtg gaatgacttt tgttactatc aaatcatttc cctgtctttc
   111721 aaatactgga tgctttttga tatgtgtgac tacatacaag tcaccatgag gtccaccatt
   111781 aggtcctgct tccccttccc cactcacttt taaacgacta ccagactcaa ctccgggcgg
   111841 aatcttaata ttgattgttc ttttcttctc aacaagcccc tcaccacggc aagtcttgca
   111901 tggattgcta atgacttgtc cacgaccacc acatctaccg catgtcgttg aaactgagaa
   111961 aaatccttgc gaatgacgca cttgaccgga accagcacag tcaggacaag tagttggact
   112021 acttcccttc gatgctccag ttcctttaca gtccccacaa gtctctgctc taggaatttc
   112081 gattttaaat tcttttccta aagcagcatc ttcgagcgtt atttccagat tgtatcgtaa
   112141 atcgctacca cgcttaggac ctgatcgtct tccccctcta cctccgcctc caaacaggtc
   112201 ttcaaagata ccaccaaagt ctccaaaaat atcagagaaa tcggtatagg ctccttgccc
   112261 aaatccaggt ccgcctgcgc ctacacctgc tttgccgaat tgatcataag ctcttcgctt
   112321 atttgcatcg cgcaaaactt cataggcttc tgttgcttct ttgaattttt cctcggcttc
   112381 tttattaccc ttgtttttat ccgggtgata ttttatagcc aactgacggt aggctttttt
   112441 aatttcctcg tcagtggctg ttttagaaac cccaagtatt tcgtaatagc ttctttcgct
   112501 catagattat ttcttatcat catctacaac ggtataatct gcatctacta ctttttcgcc
   112561 agttccacta tttgcggaat ttccagatgc acctgccgaa ccacctgtag gtccgcctgc
   112621 agaggcagtg ccttcaggac caccttgttg atacatatta gctccaatct tttgagcaat
   112681 ttgagagata gattcgtgtg cagctttgat tctatcgtag tcattgcttg caaccgcttc
   112741 atgggcacgc ttgatttcat cttgcgctaa ttgtttgtca gttgccggaa ccttatcgcc
   112801 tgcttcattc aaagtctttt ctaatgcata agccaagctt tctgtttcat tcttcatctc
   112861 agccaattca cgagcctttt tatccgcagc agcattgatt tcagcttcct tgatcatacg
   112921 cttgatttcg tcttcggata atccagaaga agattcaata cggatctttt gctctttacc
   112981 tgttccaaga tcttttgcag atacatggac aataccattt gcatcaatat caaatgttac
   113041 ttcaatttgt ggaactcctt taggagcaga tggaatacca actaaatcaa aacgaccaag
   113101 agttctgttg tctcttgcca tttctctttc cccttgtaac acatgcacac tcactgcatt
   113161 ttgattgtca gcagctgtag agaagacttg ggatttttta gttgggatgg ttgtattacg
   113221 atcgatgagt cgagtcatca caccacctaa cgtttcaata ccaagagaga gaggagttac
   113281 atcgagtaat aatacgtcag ttacttctcc tgcaagcacc ccaccttgaa tcgcagcacc
   113341 agccgcaact acttcgtcag gatttacaga tttgtttggc tcttttccaa ataattctct
   113401 tactaagtct tgcactgcag gaatacgagt agaaccaccg actaagatca cttcgtcaat
   113461 ttcagaagca gatagaccag catctctcat tgcattgata caaggaatac gagttctctc
   113521 tactaacgac tttgtcaatt gatcaaactt agctctagtt aaagtcatat ctaagtgttt
   113581 tggtccactt gaatctgctg tgataaatgg taaattgatt tgtgtagaag ttgttcctga
   113641 taattcgatt tttgcttttt cagcagcttc ttttaatctt tgggaagtat ttttatcttt
   113701 ggagatatca attccagttt gttttttgaa ttcatctacc aaccaatcca tgattacttg
   113761 gtcaaaatca tctcccccaa ggtgtgtatc accgttagtt gacttaactt caaatactcc
   113821 atctcctaat tcgagaacac tcacatcgaa tgttccacca cctaagtcat aaactgcaat
   113881 tttagagctt gcttttttct tatcaaaacc ataagcaagt gccgcagcag ttggctcatt
   113941 gataatacgc tctacttcta aacctgcaat acgacctgca tctttagtag cttgtctttg
   114001 ctcatcattg aaataagctg gaactgtaat aactgctttg gtaacagttg ttcctaaata
   114061 atcttctgca gtctttttca ttttttgtaa gacttttgca gaaatttcct gtggtgtaaa
   114121 ttctcctgaa gatgtttcaa attttactcc atcatttcca gaacgaatta ctttgtaaga
   114181 aacttttcca gcctcatctg taatttcatt gtatcttctt ccgataaaac gttttgcaga
   114241 tcgaactgtg tttgctccat tggtaattgc ttggttttta gcaaattgtc ccactaacgt
   114301 ttcacctttt gatgtaaatg ctacaatcga tggagttgtt cttgctcctt cagagttttg
   114361 aattacaaca aaatcaccac cttccataac agcaacgcat gagtttgtgg ttcctaaatc
   114421 tattcctata attttttctt tagccatttt aattgctcct tataaatgtt atttaaaatt
   114481 ttggcctagt tactctcact cttgcgggcc ttaacgcaaa agattcatta ttctctgtaa
   114541 aaacataacc tgcttgatat acttctgtta cgatctcttc tttcgcatct tcagattctt
   114601 ccgaagcaat tgcttccatt atggttgggt cgaaaggttg cccaacagga ttcaattttt
   114661 gcacattttc tttttcgaga attccattta actcacggag aatcatacca acgccttcaa
   114721 cgaaaggctt catttcttcg ctcaaattag agcctgatcc aactctttca agattatcca
   114781 gaggattcaa aagcttgata ataaaatttt taacagcttc ctttttaatc ttagtatact
   114841 cttctgctgt tcttttcttg tgattttgaa aatctgctct taatcttaaa agagaatcat
   114901 tcaaatcagc aatttctttt tttgccttct gtaattcttc ctgagcagag gataactccg
   114961 atttgccttc gttcacctct accttatttt cgtctccaat tgcctcattc gaagcatttg
   115021 tctcattttc ttttgttgct acattgtctt cagccattca caacctcctt tattatttgc
   115081 ttatttttgt aatcatctct gatacaattt tagaagtgaa atcgacaagc ggcagagccc
   115141 tcgtgtaatt catcctctgt ggtcctatta ttccaagtga accgatacgt ttgtcgccca
   115201 ttcgataact tgtggcaact atcgacacgc ctcttaaatg ttcactgtta gtctctccga
   115261 taaaagtaga aactccatcg gaggaaatgt attttgtaaa aaattccttt aataatttct
   115321 tatcatctag taaagaaaaa actgactcta gcgtatcagg atcatcatct ttaaaattcg
   115381 cacaaaggtt ttgcaatcca tctacataga gatctacatt atctcctgca tcatacagga
   115441 aggcagattg taaagtatct gctattttat aaaaatcctc aggtgcatca tttcgaatca
   115501 taagagctgg aaatagattt tggatttcat ctaaatcata acctttgata tgatcttgta
   115561 aaaatcttga tattttatag agagactcct gtgagacatg atcctcaaaa aataacttat
   115621 gatgaagaac cacaccggag cgagttacta agatcataag cacttcagca ccatttacat
   115681 gaattagctc aatatgtttg agtgtatcaa aattcttttt aggtgctaaa acaaccccag
   115741 cactgcaaga caaaatagaa agcacccgac aggtaactgc cagaatttgc tctaatttca
   115801 actcattttt taagtattct tcctgaattc tttgcttttc tttcagactt aactcgtata
   115861 aagagatcag attatctaca tagattctgt atcctcgttc ggtaggaaca cgtccaccgg
   115921 aatgatggcg ggagttgata tatcctgcct cttctaaatc acggaataca tttcgaacgg
   115981 tagctgggga tagaccaata tcatattttt ctgatagagt cttagagcca acaggcttat
   116041 tgtcagatac aaaatcatca attaggactt ttaagatcat tctatgtcta ggactcagtt
   116101 ccattcgtat tctcctttta gcactctgaa ggttagagtg ctaatatagc taaaaagttt
   116161 acaaaccccc tctatttacg tcaagttttt ttaaaaaaag aaaagaatgg aaaaacaatc
   116221 tcaattcaag agacataatc gactagatat cagcaccaaa gacaagaaaa attacgaaaa
   116281 ggaatataga aaaaagccct agatagaaaa agagacttcc ttctaagtga gaaaaggtct
   116341 ctatggtatg tattctaaac tcatcctttg atttatttaa aaaattggga gctcttattt
   116401 ccttttaacc acagattttt tttagctaga aaatccgaag caaatatcta aaaccgttag
   116461 ataaatatat agcaataaaa aatttctttt ctacctataa ttatctacta aagaatgttc
   116521 aataaaaaat ggaatgattt catataatac acaaaatata aaaacaaggt aattttgaat
   116581 aaatgaattc gaaattaatt ttctgtttca tactcattac ttagtatttg gagtctttcg
   116641 taggattttt actgtataat tttatttcag aaaaaaataa atttacattt ctttgtattt
   116701 tgaattccta tattaaataa taccctattt ttataaaatt gatttagttt aaaagatgaa
   116761 tactttatat ttttattatt cctattttcc aatttgtaaa aatttaagga aaataatttc
   116821 aaggttgaat cgcattgatt aaagttctct gtatagacga tgatgcaatc acattgatgc
   116881 tttgcacaac cacaatcaaa aaaagtggtc tttcgagtga gattataaca ctgcttaatg
   116941 ggcaagaggg tgttgactac tataataaac taacgtctac aattggatat gaaaatcata
   117001 gcttttatcc tgatcttata tttcttgatt taaacatgcc aatcctaaac ggctgggaat
   117061 ttttagatta ttttatggaa gatcactatc caaaactaca aaagacaaag gttgtaattc
   117121 tatcttctac tgttgatcca agagacaaag agaaagctaa acaatatcca atcgttatcg
   117181 actttttttc aaaaccaata acaaacgaaa tgttaagcaa attgagttat aagctaaact
   117241 aacgttattt caatcatcaa taagcaaaat atatttctag cggctaacgc ctaatttgca
   117301 tctaaatctt taatattgga aaacttgcaa tctctgtgcc tttaacaatt tccattctat
   117361 tattttctct tctctattct tgattctact gattcaattt tctttgcaat ccatatctgg
   117421 tcatcgtcag ttaattgtct aaatgcaaag ccagttccct cttcgtcttt tctaactact
   117481 ccggcagaaa gaaaacgtct atcggaatct gtttctattt caactctaac ttcttcattt
   117541 atttcaaaag gatcatcttt ccaatccaaa taaaaccctg cttcagatat atctcttgtg
   117601 agtttatcta tggagttaat acgaacttgg atttgaattg gatttctttt ttctcctctc
   117661 catccccttg gatacatttt catgtaagga gcagaaatat ctttctttaa aaagtagaaa
   117721 atagcactaa gagctaagag tgtttgaact aacgcgccaa tattgtataa atgggggtct
   117781 ttaaaaagtg taatcgtatt actaagaatt aatgcaatgg aatagaacag aaacaaccac
   117841 caaccccatt tctttaccaa taataaacca atcccgacta acattggaaa aagcaaaaga
   117901 ataatttcaa ttctacccag ttcccgtaaa atagatttaa tttgaaataa tgaatagtga
   117961 aaacgaattg atagaccaat atagttgatt aagggtaacg ttaaaaagag tattccgaaa
   118021 aataataatg ttttcggtct cttcattctc tgtcttcggt tcgtcataaa cacctcataa
   118081 attctaccgc tttggatttt taaatacttc cttggttctc cgtcaagagt tttggaataa
   118141 aatatggttt ctagctttct ttgtacaatt cttttttcct tacaccgaac tctccttatt
   118201 taattaatat aaattttaaa atcctgggtc agcgttacct ttaaagctaa ggataatcgt
   118261 tgttattctt tgtattgtca attcattagt accagttcca atttgactga ataaatttga
   118321 agtgtcttgt agtaaaggat acgagcctgt attgtatgaa tttgtatcag atgaaattcc
   118381 ctgcgcaaca aaatactttg atgagttatt aacatttgaa tttattgtcg aatcaaagta
   118441 gccataattt aaagtatttt gatttgaaca taaagtatct gcgattatat tatcattgta
   118501 tccccatttt gctactgaaa ggcttttccc atttataacg tttgttatag ctgctaaaga
   118561 ataaaagtca gaatttcccc ctgcgcaaac atacccctga tttggatttg agcgagctag
   118621 taattgcata ttgtatgaat ttgaaattag gttaaaattt gtttttatca ttacattatc
   118681 tacgtttcca ctgaccttaa aaactccatc aacccaacta caaatcattg aagtattttg
   118741 tgagtccaat gtaacggtat agcattcaac tttcaaatta gcatttatac ttgagtcaaa
   118801 tgcactcggt tgaaaagtta acaatgaccc tttcccactt gttctttgaa ttgcatcatc
   118861 atagaaaagt tctaaaactt ttagattatt tgttatattc caaatttcaa cactatatcg
   118921 aaatgtttta gttcctgaat atgcgttgga cgaaattact ccgttaaaag ctggattgaa
   118981 ttttatttta aagttgtctg tgctttgcgt tgaaatgctt gtcttgccta atattccatt
   119041 tacatagaat tgagctaata tattatcaat attatccgta acttgtttta gaaacgttgg
   119101 catttgcctt atttgatccc agttatttaa aatagtattg tttccagaac tggagtttga
   119161 actaatatta tttaatccaa tagtgacagg cagttttatt aaaaaaccag atgaactatt
   119221 cactttgttt tgagatgctc tcaggagtaa aatttgcaat ggaatatttt gattcgattt
   119281 atgattggtg caatatgaaa ataatagaat attgaccgaa ataaatgacc agaataaaat
   119341 gtattttttc ataaatacca ttaattaact aatcagaata aattactgaa atagagggac
   119401 tattagaatt aaacgatata gtagaaagta ccaaaaaagt attcgcctga ttaaaagtca
   119461 aagtagcact cgaagttgta gatgaagcgg tgtaagcact tgtaagaata tttgccgtcc
   119521 gagcgttgat tgcacaaggg tctacatttg aaaatactgc aatagaaaat aaaccacctc
   119581 cattacttcc ggtaaatgtt aacttcccat ttacttttgt cgttactctt atcattgagt
   119641 aactttgcaa agatgttcca aatcctaaat cctctgccgc atactttaaa ctttgagtcg
   119701 tcgtcgccgc agtatataga aacacgttag tttgtattcc tcctgatgaa atatatgagc
   119761 aagtaggtgt tgtatctact acaggcttat ttgtgttagc ctgttgatag gataacacag
   119821 aagctagaag taatttattt ttcttctttt cactagaatc attcttgctt gaacaactta
   119881 aaaataaaaa gattaatatg aggtttgcta tttttcttat attttgatta aacataattt
   119941 atttctcttt atttcaaaaa tattttattg aacgcttact gtataacctg ggttgcatcc
   120001 tgcctttatg acttcatgga tatgctggac aaagtccaaa tgattatccg agatggaacc
   120061 acattcaaag ccaacagttt caacagagca ggcaagagcc tcacaaaatt tatcagttac
   120121 cggaaatcct agtttgccag ttggcccccc tagttggtca tatctgactt gatgcaaccc
   120181 tgtaacaaca tgtggtttcc caaattttgt gccagaatca gctgatggca catcataaag
   120241 ctgactcaag taaatagcag aaccatcttt ataacgtctc atatatcctc catattgccc
   120301 atatccattg actgaatctt caagcttgct ttttattctt attgttggag tggcttcttg
   120361 tccaaagata cctacatcct tagatttctt tttaatgatt ttaccaggac cgtcattatc
   120421 cacatttact attggatcaa tataatcgtc tgttctgttc cagcccaatt cttttaactt
   120481 cgttccaatt aacgtactcc acaattcata gccctttggt cctatatgaa tcggatcacc
   120541 aattagaagg ggtctgtctt tgtgtagtaa atcatgaaga agtgtattat atttatcata
   120601 aattccgtct ttaggtaccc aaaaattatt tccaaaatta aaatcccatt cattagaaaa
   120661 ataattatat agcccaaatg cttctacata ccctggataa gcggacttat atgcaggtcc
   120721 tatatcatat gtaactttat catttacaca tgcctggttg atacttgcta gccttaataa
   120781 cgggctagct tgccattcag cctttgcctt tttatctttt gataattcat tttcaattgc
   120841 tgctgctgtt gctacgcccg ctgcagaatt atctgtgccc attgctactg ctgtacctcc
   120901 tacgactgct atgatatgaa caatattatc ttgcgctgct tgctccaagg cttgaccacc
   120961 agcagtgacc gcatctccta aatttccagc aaaattgttt agattcatcg aattaattga
   121021 atcaaaaaaa ttatctgcac ttgtagattt ttttcgcatg acctttttag taaaatccgt
   121081 aaacgctttg gcaacctttt catcatagtc tttagcagcc tttgccatcg cgtaacaaac
   121141 ttgcggtccc aaagcacaag cccaccaagg tatatcttgc ggtttaatga atgactcagc
   121201 taatggtctg cataatgctt gcctatttac aatggtggaa tcataaggat ctatcgcacc
   121261 tgaataaaca ggaattgtac cctctaataa aacctttttt ccattttcta aatgccagtc
   121321 tgtcataaat gtaatatttt ctgttacaaa atctacaagg taagtattta gagtaggaag
   121381 cagacctttt gtttccatat taaacataat atcattacct ccaatcatta atgtagagtg
   121441 caatgctgat ttaccgtttt gaaaatgagg aggttggttt gcaacaggta aaaaccaatc
   121501 agccactttt atttcagaag ggaaaggacc acccattggg catttgtttg ttgttgggtc
   121561 aatatcccac catccgttta atccgtcata ataagtatta aatcccatca gtggcatcac
   121621 acttgtagtt tggtaagttg ctatcccata atttcttgta taaacgggga ctaatggcgg
   121681 ttgaccgtaa atagaaaagt aatcgctcca agaatcaatc caaatagggc tacctccgta
   121741 tatcccacca acgggtggaa taggaacgtg accccaaagc accaggtgtc agcacaccat
   121801 agaaactcac aaagtctgat agactatctc cccattttgt gacttgaaga gggtgtcctt
   121861 caaaattgct actatcatgg ttctctttaa tcttttgctt tatctttgaa ctagaagaat
   121921 tcaaatcagc gttaattgca gctataatac gagcacgatt cgcgagtgca gctggattat
   121981 tcgctaatac cgtctgggat tttcgaagat agtcatctaa tagcttttca tcaatttgac
   122041 tgtggaaggt tcctcctaaa tctgcaacgc taggtgataa agttatatct ataaaattct
   122101 gatcgcaagt aagcgaatta tgatcaccag gaaaggcaat tagggactcg attgtaaaag
   122161 caataaagat taaaattttt aataagatat ttttcatatt ttttccttgt aataagtttc
   122221 taagaagaga tgcatagaat ataattgtat agatttttat taagcggacc cgctggactt
   122281 ccaccagtat tactaatatt gcgggagcca aaatcataca tccaataata gtctgtagag
   122341 cttgatggaa tttcatctga ccgactaaat aaataatctt ttaaattata aatactagtt
   122401 ccaagagtga aatcaaggtt atcccaattt cttcccactg ttttatcatt gacacaactt
   122461 gctcttgcag gactcaaagt tttactctgg tttccacttg aatcatcaca agcagtatta
   122521 tttgtagggc aaccttgaaa cttttgtgca ccatagtaat tagccgcagt acctgtcccc
   122581 ttacagtcat tttgtgcgct gcgatagact tggccctgaa tacatttttt aatgtaatat
   122641 tttattgaaa gtatcgattc ccttttagca aaagggggtg gacatccatc aacacctagt
   122701 gtgctatagt taactaacgc aacagttcca tcattattat caacaatctt atatccactc
   122761 ggtccgccaa gaaggtttgc aaaataacac atttgagtta ccttagaata cagggaatcc
   122821 ttttcagtct tttttccaat gattggtata ttactatcgc atcgattaaa aaagattatc
   122881 caaagtaaaa gtaacaggat tatttttttc atattatttt ccctccaatg catcaatagt
   122941 aagatttctt agtttcagat gcctatctat catggattga ataaagtttg gatccatttt
   123001 atgataccct tttaatgcat cttcttttag cttaactgca cttacgattc cttgcgttag
   123061 caactctctc tggtgttcta aatcttcttt atcaaaggta ggtgtctgca ttttaactaa
   123121 gttataaaac tcatcgtatt ggtcgggtaa cgttcttttg gttcttttaa agttataatc
   123181 agagagacta tctaagaatt gaccacccct ctttcttgca tcttcatctg tattacctgc
   123241 tatggcatta agcgaattaa aatcctgaat acttttcata tccccatagg ttaatgaata
   123301 tttaccgctg gtagggaaag gtaaatttaa tgttccatat aatgtctgta ttccagtaac
   123361 ttcatctgat ccgatttgat cgattcttcc aatttgggga aatttataca ttacacttcc
   123421 ttcttttgga tcactacctg ttcgagcctg acaatctgta actgtgtctc ctgttttgca
   123481 atgaccaaga cctgccatat atccgaatgc cttcaatgct tctgttttta aatttttaac
   123541 atccccatcg cttcctatat gattccaatt aaaatctgaa ttattcagtt gaacatctgc
   123601 atgaacaatg cttccattgc agctccaaag atatacacaa cttgaagcag tgcaatcagg
   123661 atcagagtta ggctctgcaa aaaatactgc atctccttgt tgaacatcag tccttgcagt
   123721 gcaatctata tcttcaactg atgcgggttt atccaatgtt atcccttgaa atttaaaaga
   123781 aaaacgatat ccgccttttt ggaaccattc ccctgctgca tctaaaaatt cttttgcaat
   123841 caatggttga ttcttggaac ttttaaaccc aattatttta ggattaataa aaaatcctac
   123901 tactggattt aaaccactcc acttagcttt actctcagat aaatacatcc aattcattgt
   123961 gaagaggata atcaatatta cacttagctt ttttttcata gttttacgtt tccttgctcg
   124021 ccattataat ccagttgaaa agtttagagt tttaacggct gaattgattt gttggtcacc
   124081 gttttttgaa tcaattatca ttatttgaaa agtccaactg ccttgcgtat tcgtaggacc
   124141 tccaaataat aaacccgtcg gcgtaaggga gaaaccagga gggagattac cgctaatgat
   124201 ctgaaaagta aatggtggct tgccagatgt aggttgcaaa tatactttat tgtgttcaaa
   124261 ccatctactt ccacaagcag ccgatacaca tgtatcaaaa tcgggagggt cattgatttt
   124321 tgcagttggg atatctgtaa ctgctgtgac gacactaacg cctccaccta taatcggagt
   124381 ctcgtctact tgaataaatc ccttttcaaa taaagtaagt ttaaaattac aaaatgcgcc
   124441 aggtccccaa cacattgttc cagtcatggg aaatcctaat tttccttcag gaccaccatt
   124501 acttctataa acagaattta ctttattggg taaatgaatg gcaccagttt gatatttaac
   124561 gttagctgta ttctcaggtg tatttgatgt atataatttt aaatagattc cgcttccgtc
   124621 tgtgtattcc ctataatatc caccataact tttaccatca gaagacacgg taacacctct
   124681 gggagttgcc tccattccta ttacaccaac attacgagcg gttttcttta ttatccccca
   124741 tgcgtcattt aactcaaggg atacttcaac gggaatactg tttgggtctc caggagtatg
   124801 ataaccaaga ccggcaattt tgaattcccc atcgcttata accttccggt cctatatgaa
   124861 tagcatccca aaatcctgat ccctgaaaaa aaattccggt ttgcctaaat acaatatcct
   124921 ctggaaccca aaaattactt gcataaggaa tatcaccagt tttattgaaa ttactataca
   124981 ggggataccc atctacgttt ttaggaaaag actgtttgta taacgggagc agattatttt
   125041 taattctatc attcacacaa gcttgcgcaa tacttgcgct tgtaaataat atccttgtgg
   125101 gagtatcatt atattggtta tctggcgacg ttcttcgttc aaaaatttgt cgcttcagtt
   125161 ctgctttttg agcatctgat aataccaaat tattatttgc agtaaactct aaatactcat
   125221 caagtaattt atccaattga gtatctaacg cttgatttgc tgaattaaca aaaccaccag
   125281 gagaagatgt attcggtttt ggtggtggat tataaatatc tgagagaaat tttgttacct
   125341 cactgattac atgttcattc catttaataa tgcttgtcat catacctgga ataaatgcgg
   125401 cataccaagg aatttcattc aaatcaacta ggaggtcatt cagtggtcga caaagcgtct
   125461 gacgatttat cagaacagta tcatatggat ttcttgcagg atgacttaga acagggagtg
   125521 ttccttctaa gaaaatctgc tttccatttt caaggtgcca atccgtaatt gccatgatat
   125581 tcaatagagt agaatctact tgatacctat ttaagaatgg cagccaaccg ttaaacccta
   125641 gaccagattg aataatatca tttcctcccg ccattaacgt tgagcgaagg gaagattgtt
   125701 cctttgtaat agcttcatag tagtcaacag gcaccatacc tgtcgccatt ggtctcgttc
   125761 ttacacatct aggtgagtct ttattcacag tccaatcttc tcttgtccca tcaatattaa
   125821 taccaagcgt cgtcattacg gaagtagttt gaaatcctga aactccaaag ttttgaaaat
   125881 tcagttttgc attcggatct aaaattccgg cgccagcagt catataatca gtccaagaat
   125941 ccataaaaat tgatattcta ccaaacgttc cagccgcgtc tccgtaattt ggaaatatat
   126001 ttctaggtat ggcaactccg taaaaatcta ctaagtcaga taaactatcg cctaactttg
   126061 ttactcggaa gttcatgtcg tgattattcc gaacaatcgt ttttacccgt tcaatggcat
   126121 ctttttgatt ttgtattttt ttggcttcgt ccgttgttgc aaaagaacct gtagatgaat
   126181 attgagcttt aatatcgtta cgatttctgg gattattata atcttgctca ggaatacaac
   126241 tcaaagaatc ttgatcacct ggccaagaga ttacttccaa aattaaaaaa aaataaacta
   126301 aaataagaaa taaaaatttc attaaatttc tcccgtacat aatatattaa tcgcttcatt
   126361 tttataagct agtgaacttt caaaagcaat tgctgaaaaa actttttcat tatcattaga
   126421 ttctagtgtc caaacattac cacttacaaa acctattttt gtccaatcat tagtaacaga
   126481 agggctaaaa agaactcgta agccttcatc tttactcctt agcctccact tcttaccagc
   126541 aaaagtatcc gttgcacatg cttttgctgc ggggctaaca gtaggatccg ctttgccatc
   126601 tttctcacag cttctatcgt tagtcggaca ccattgtaac tgacttgctc cccatagtgt
   126661 atcttttgtt ccagtccctt ggcagtcatt ctgcgcagac cggtatactt gaccaatcaa
   126721 gcagcggcga attaggattg tcttctggct aatgacttta ttccccaacc aactatttgt
   126781 ttgcagtaat tgatacgtat aatttaaggt gccgtcttta ttatctacta ccgttcctat
   126841 ttcttttgat ccccaagaca aaccaatcaa ttcgagtaat gcagttttat tatcatcatt
   126901 acg
//
LOCUS       DAOQOS010000002        95285 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_69_length_95285_cov_6.747590, whole genome shotgun sequence.
ACCESSION   DAOQOS010000002 DAOQOS010000000
VERSION     DAOQOS010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 95285)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 95285)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..95285
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69_length_95285_cov_6.747590"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1004)
                     /locus_tag="PLF66_00625"
     CDS             complement(<1..1004)
                     /locus_tag="PLF66_00625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62563.1"
                     /translation="MSKPILLWFGILFFFIISILFFNFEGMEIGFDLDPSYMWATSYF
                     AFDDTVIGSIPIFTYGPLGFLIIPTVYSYAILSSFIFESYCNLSIFFFLNKSLNKKPF
                     FPNLLIHIIAFGSILFFPIDHRIMLLCYLVALETYKNLRIVKSLHLFTLLFVLVFIKS
                     MYLFLILSLLPIWLYLFYRKKNRTLLIVTPIVSFFLLALIVRIIFNYSLTDLYNMFRG
                     YLEFSIANSSAMTQADDHTSIKWVYFFSYIFLTSLLWVSFRKKTAYPFFFPSFLIGNF
                     IYFKYAMARSDHAWVFIYLAVIFYIIFFIYSGNYFKTKTYLISHISYLISHISCVSLH
                     "
     gene            1216..1464
                     /locus_tag="PLF66_00630"
     CDS             1216..1464
                     /locus_tag="PLF66_00630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62564.1"
                     /translation="MQNAYVLTGSLKSPTLIELDESLSLSLQKVRITIEPLQSVFNKK
                     SLLLSLKKIQAKQEKRNYTSPRKEDVDLYIAKLREDWN"
     gene            1543..1848
                     /locus_tag="PLF66_00635"
     CDS             1543..1848
                     /locus_tag="PLF66_00635"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HMZ62565.1"
                     /translation="MLPEDAQLFVSDLTRMECRVKPIQDNNALLLEEYDLFFSESVEI
                     VNLTNEVINKATEIRAKYSFKTPDSIHLASAIINESNFFITNDKRLSSFKEIEILKL"
     gene            complement(1976..2329)
                     /locus_tag="PLF66_00640"
     CDS             complement(1976..2329)
                     /locus_tag="PLF66_00640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62566.1"
                     /translation="MLNEREAKKSSDEKSSFSFFSKKTLILHLIFLFLIFLLRCDKTP
                     VSSGAKSDDLKLLFYNVRSVTTSTATIAWSCSSPSQGYLLYGAGNGAENTAFSIGKNK
                     NHFVILLRPLQTISS"
     gene            complement(2424..3059)
                     /locus_tag="PLF66_00645"
     CDS             complement(2424..3059)
                     /locus_tag="PLF66_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017863852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62567.1"
                     /translation="MLQFQCSSIKGYLINRGNDFIDIVNIGFEDNIYGGGIYIDSIMF
                     GLAYGAKDSSGVGLRFGHLGMYQSNDSESNNFLKAGSENGPCRDSRYGTFGQLQFLFF
                     ATSNYHEPRSIIMVRNYRKNIAFMNVLLYGASKRDSSKGLLLCYRGKKYHTIIRDSYV
                     VLPMEVSFGLYYGIRLGFNPHELLDFIVGIFGFDLLDDDTTTVYVELPVPM"
     gene            3969..4724
                     /locus_tag="PLF66_00650"
     CDS             3969..4724
                     /locus_tag="PLF66_00650"
                     /inference="COORDINATES: protein motif:HMM:NF015739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HMZ62568.1"
                     /translation="MNQEHLNRREWLAKNNRLRLGAIFIIFASLLIIIYDFGFTRKSG
                     LSSSVNKLVITGHQKLSKEEIVAILGVQAGVPFEDYSLKSFEEKLEKHPRIKKASVTR
                     RSKEQLLVSIVERGARFIINSNDNLYEIDDDYKLVSVDDIRDTNLIVLSGEFNIGQNQ
                     QVSKKLKEFCESVENLFETYPQLRDRISEVRLDQDGEVTVYTHYPQRIRVNMGNKLES
                     IQIRKLYASLAFFENQAVNVRLLDLRGDDAVYQ"
     gene            4730..5977
                     /gene="ftsA"
                     /locus_tag="PLF66_00655"
     CDS             4730..5977
                     /gene="ftsA"
                     /locus_tag="PLF66_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012387347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HMZ62569.1"
                     /translation="MQELDNSIAAIDLGSSLIKVVVGRAVSESEIEIIGTGTASCSGI
                     KNGAIINIETTTKSINEAISLAELMAGQEISQVIVNITGKTIKADNSKAVVAITNRER
                     IVTDDDIRRVIEAAQPRVPSDQMVLHVLSKEFAVDDQTNIKEPLGMTGVRLEAEVHVV
                     TAGITTVHNLDKCIEGAGLEQIDKVLSSFASSEAVLTSGEKDLGTAVIDIGAGITDIV
                     IYQDGGVCYSGVIPFGGNNITSDISIGLKTPLEQAEIIKKKFGHCMHEAVEPTEKIEV
                     PAVSGRPSRQVLRQDLVRIIEARTREILEHINHELDRSGKKAFLSGGIILTGGTSLLP
                     GIDLLAEEVFGLSVIVARPAGLGGLSERVASPEYSTAVGLIKYVVRSIDLDSKAPSEK
                     WSSSPGEKESPLKRVWRWMETNL"
     gene            5998..7212
                     /gene="ftsZ"
                     /locus_tag="PLF66_00660"
     CDS             5998..7212
                     /gene="ftsZ"
                     /locus_tag="PLF66_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020776008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HMZ62570.1"
                     /translation="MLYMDEQRTSPVVIKVVGVGGGGMNAVSRMTNANFRGVEFIVMN
                     TDEQVLSKSSVENRVQLGNKVTRGMGAGGDPEIGNKAAQEDKDRIASVLKGADMVFVT
                     AGMGGGTGTGAAPVIAEIAKELKCLVVGVVTLPFGFEGRRRNDLAKNGLNMLRQNVDT
                     LITIKNDSIFKVVDKKAPIDYAFRVIDDILLNAVKGISDIINHPGIINVDFADVKTIM
                     KDTGDAIMGVGEGVGENRVAEAVEHAINNVLLEETSIAGASSLLINVTGGNDLTINDW
                     NEVSQIITSQVDTNANIIIGLNEEEGLNEKIRVTVIATGFRARLPHTLTSLKRDATPV
                     ISQQQNTQPQKEQKEAQQQPLVRPKVVNMPDPHEYIEPTRSIKTPARVNSPYNYNEDY
                     DIPAYLRRGNRD"
     gene            7501..8262
                     /locus_tag="PLF66_00665"
     CDS             7501..8262
                     /locus_tag="PLF66_00665"
                     /inference="COORDINATES: protein motif:HMM:NF025160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HXXEE domain-containing protein"
                     /protein_id="HMZ62571.1"
                     /translation="MNQKPILSSILLLFAMLWLPLGQEEFLLMNWMKIGTYVIPFLFI
                     ATTAFRQSSTKNIFQDTKLISVLLLIVYLIHQYEEHWIDLFGNEYSFYYSVNQMLSDI
                     LSPSSPISPLTREAIFVINTSLVWLVGVNAIWMSETRIFPTFAMTAITLVNAITHVIA
                     GIVKWSYNPGLVTSILLFIPFTAFYFHFKLKENSNLKKQIITSILWAVIAHILMVSGM
                     LMANYFHVINQTVYFVVLILWSILPIAIFRTVNLD"
     gene            complement(8266..13932)
                     /locus_tag="PLF66_00670"
     CDS             complement(8266..13932)
                     /locus_tag="PLF66_00670"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HMZ62572.1"
                     /translation="MINLNGYSISEKIHEGEKSILYRGKKKDQPVIIKILNNDYPSTS
                     ELSKFKHEYDILENINSDYIIKTISIEKYKNSIAIVFEDIGGESLATLYNSVSSYSIK
                     EILNIMILSIKALGEVHKLNLVHKDIKPHNIIFNKSSQVLKIIDFGNASLLVKQNSFI
                     SLNSSIEGTLAYISPEQTGRMNRTVDYRTDYYSLGVTFYRILTGGLPFLAKDPIELVH
                     SHIAKNPISPFQKNKTPKPISDIIVKLLQKNPEDRYQSSSGILYDLEKCLKLLEEKGL
                     ASLDTESFGIGEFDFSPKFQIPEKLYGREKEINQIIDTFKKVAEGNTELLLISGRSGI
                     GKSVLINEINKPITKYKGYFASGKYDTFKKTIPYRAISYAVQSLIQQILTESSTSIQS
                     WKKNILDAIGTNGKIIIDVIPELETLIGEQPPVPELGTTESQNRFNIVFQNFIKAICS
                     KEHPIAIFLDDLQWADIPSINLIQALLSNPDLKYLFLMLSFRDNEVLPKDPFSIMLDD
                     LKNGGLKFNKISLEPISLTDISSLVSDTLNSADIIAKELAKVLFEKTKGNPFFVNELF
                     KSFYEKEFIQYKNNNWIWDIKKIQDVKISENVIDLMLEKVKELSDSAIEKLKLAACIG
                     SWFKQEVFFKILDKTESEAKEDLLLLANEGFLRLGEFDVNFVHDKIREATYTLISETE
                     RSRYHYTIGNTYLSMLDKYKLEDHIFTIVNQLNQGLAYITSEEEKVKLKELNRIAGNK
                     AIASNAYDAALGFYQNSINQLPENSWEDNYFYTLDLYTNRARSEYLSKEYLSAEKTFD
                     LILTKAKTPLDKIVIYELKSSMYVSQNKMLEAIVILKQALKSFGVNLPNNPTEISPLP
                     EIIKFKLKFGKRQTEDLLNHTLTTEKKSLAIMGLLNALSAPAFLAQPALFPVIVLKMV
                     NFSLTHGNTPLSPFAYAAFGIIQGSALGDFEAGYKFGKLALDLIKKIGEQTKPIECRT
                     YFLFATMINHWKHHAKENKALYNHAFEKGMEAGDLQYGSYSLNNIFFQGVMIRENLSI
                     LSENFIKYRKSILSLQQHNAYQLYQLNEQFVSNMRDLTENKLMLVGEFYDETKVLPEL
                     IESKNANALFDFYVCKTRLEYFFGDLHQALEYSSLASTHEAAMFGMMFVPEQVFFDSL
                     ICAKLILNTKDKKKSKDYKKRLDKNYKRIKKWGENCQANYGHKLHILQGLIYQIENNQ
                     DKALSSFDQAIELSQQYDYILEEALANEFIASIWLERNKEPFAYIYLNAAHYAYKKWG
                     CEPKVKQLESKYPQLKRQTKHKFNNESSIDDSMTISSSATNNNMTEGNFLDLNTVIKA
                     SQTISGEIQLAKLLEKMMKILFENAGAERGFFILKDKDKFYIEAEGNANTDTIEVLKH
                     ISLEGYNELSPNIVNYVTRTKGIVLLNDAVKTGIFINDSYVKEKKPKSVMCYPIINQG
                     NLLGAVYLENNLTTNAFTPDRVEILKILSSQIAVSVENSILYANLEEKVEERTRDLNQ
                     ALTEVSALKEQQDGDYFLNTLLIEPLSQCKAFSKVVHIESFIKQKKSFIFRKNEYELG
                     GDINITEIIELQGKKYIVFLNGDAMGKSIQGAGGVLVLGTVFKSIIQRTISTASGRNQ
                     YPERWLKNAFIEMHKTFEAFDGSMLMSIAFGLIDEETGTMYFMNVEHPDIVLYRDGIP
                     HFIKPKNQYRKLGTQGQTGHISIDIFPLLPDDIILLGSDGRDDLIIGKDEEYDLINQN
                     EELFLDHVKNGNGDLEKIFESITNAGKLMDDLSLLKVHFKGKLSNVEKLKEDLKLFEK
                     YKTESQFERIAELGKKLISDYPHVSDYLYDTALAYRALGKYQDSIDFGERLRLRDSKN
                     ISNLLTLIESYKSLGQKDKAFPLLEICLKEKPEDIRFHKLKNEFGV"
     gene            complement(13952..14716)
                     /locus_tag="PLF66_00675"
     CDS             complement(13952..14716)
                     /locus_tag="PLF66_00675"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012389586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HMZ62573.1"
                     /translation="MKFKNKVVIVTGSTSGIGRAIAIKFSENGATVIVSGRREVEGKK
                     LENEIIQNGGKCLFVPADVSKEDDIKNLVNQTIHKFGKLDIAVNSAGISGDLFPVTEY
                     PLDVWNQVIAINLTGVFLSMKYEIPEIIKQGGGVVLNVSSALGLRGKELVSAYSSSKH
                     GVIGLTKCAALEYGKYGIRVNAACFGGIETEMDQEFYKKSDNPEQLKKERLKSYALGR
                     MGNVNEVANTSIWLCSDEASFITGAAIPIDGGKTSK"
     gene            complement(14751..15431)
                     /locus_tag="PLF66_00680"
     CDS             complement(14751..15431)
                     /locus_tag="PLF66_00680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62574.1"
                     /translation="MSKTKTIQYIFLHLDGVVLENILAPITRNLIEKLGGEYTAEIEN
                     NVFARTQIHAADYIKEKLNLSHSIEELIQLYYSERKKYESENSIQLKEGIQEVLSLFK
                     SLGYKVVSYGGASKEYFTKNTSNIQKYFDDEKYIQTKDIRPGIKEITKDIYNLQFQEV
                     LFIDEEKNVAEAAMRYNTPFIGVASHKEFSFQRIEMEKIKVKYIVNNLYEINSFLLNK
                     IESDVYRT"
     gene            complement(15431..16720)
                     /locus_tag="PLF66_00685"
     CDS             complement(15431..16720)
                     /locus_tag="PLF66_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020776492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AfsA-related hotdog domain-containing protein"
                     /protein_id="HMZ62575.1"
                     /translation="MIHEFEKLEKFDIPNVIVLDKQYTRTYFQEDSLISNIRRALQRE
                     ISVDLFEKIIHSQISEEEDQFLLNYYEKRKDKDGIEAYLLRTIPQRITRERASQYTEE
                     GSITEEEKEYLFKFYTLDEGQQRYILQNNLTEADEIKILKMFNMRSLHINNVQKAMIS
                     EILEKVEELPKKEVFFANLYTPPDHKFFSPPNLKHISGMQITEAARQFAIACCHKFGK
                     VPFEDVTFLLQSLTSEFFQYAKVNMPIKMRAIAKDLKLNKDKSWSYVDIEISIYQENV
                     EISKVKTVATILPLKVYKRLKTGQEEVYEIDPRFRLSEKFKNNISVRYLDGDKTKKWV
                     CHIENFSKKGFMVHSEGADPPRQMANNDHLEFFMHFDLAGFVHGRCKMIWMKSDTEQE
                     DNYYSGFEILDMTSIDQENLNEAISRYGRLIEEREIF"
     gene            17159..17557
                     /locus_tag="PLF66_00690"
     CDS             17159..17557
                     /locus_tag="PLF66_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002724461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62576.1"
                     /translation="MSPEEKEIFLQMQQNINSILELIQTTHEPNNGYKIVLFLVPIFG
                     IVFGSTLLCLIFLFWHRQRMELIKSGLYKPIAFDFRAYSFFLGLLLTFTGLTLAIVFI
                     IVLGKSLAMLGGLIPLAIGLSLLTYYKFSR"
     gene            17569..18156
                     /locus_tag="PLF66_00695"
     CDS             17569..18156
                     /locus_tag="PLF66_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008589946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HMZ62577.1"
                     /translation="MLCNPADWDCIQNVLAGDFSAFEFLMDKYQGMVYSQALKFTGNQ
                     EEAEDLTQEIFLKAFEALSTFKGESQFSTWIYSIARNQILMKHRKESTNPKISIQDME
                     EVHNKYFDKIKKWKDQLNPEMQILKQEMNSKIQSMLSRLPSNYKKPLILYYFENMSYN
                     EIAESMNIKVNTLKSYIFRGRELLKEWMQKDNEKG"
     gene            18143..18481
                     /locus_tag="PLF66_00700"
     CDS             18143..18481
                     /locus_tag="PLF66_00700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62578.1"
                     /translation="MKKDKKEYFTKPNIYSVFEKSPIDIKKNFFYRVIPYTYIKYSIY
                     MMTFLFFSPILVVLFNSDLRKNDAIFYPILLTLSFSFLLFSLLSGAILFFYKIPYMQE
                     LKDKFGYTER"
     gene            18624..18917
                     /locus_tag="PLF66_00705"
     CDS             18624..18917
                     /locus_tag="PLF66_00705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62579.1"
                     /translation="MNESEAFRVIEKLRKEEASELITQIRSVVRKDYKEIDKFYLLIS
                     HLETIKAIEEEQARLRSLNLVYGLGLFLVIGLLLYVLVSQRKTIQNLNQLLKE"
     gene            18917..19846
                     /locus_tag="PLF66_00710"
     CDS             18917..19846
                     /locus_tag="PLF66_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000834725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62580.1"
                     /translation="MKYFSIEEIQGQDQALQYIKHYVKNPHEIPPLLIFHGPISVGKW
                     ALAERFARHILCLHGSSCEICPSCKAFMNNHHPDYIVFPANQKIAIGEDKDPAEFTVR
                     WLQNRRLNYVPHLSKYRIILFPDASLINNEAESALLKSLEEPPSHSRFIFIVDDLLKL
                     KQTIISRAVCVPFQYLSKAILQDLSANLQLYREDFFGGSLIPYDIPSEAITLIREKVS
                     QNLNDSLLLLQLEMWIREYKEDHPEWPEDFDYIEFLELISTIMIYEFSKANFEENVDK
                     IEILYEFKMELHKNIPGLENFLLSDLFHKLTRD"
     gene            19854..20891
                     /locus_tag="PLF66_00715"
     CDS             19854..20891
                     /locus_tag="PLF66_00715"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HMZ62581.1"
                     /translation="MVQDNFYNLTPDLILDLLEKNGFSPTGHCFALNSLENRVYNVRL
                     ENNEVAIVKFYRPGRWTKEQILEEHSFLFELDKNEIPVCAPKVFSGKSLFEINGIYCA
                     IWDRTGGRAPEELSYEEYQMLGRYLGRLHSVGANQKTKYRNHFTSQLYGIDSFAFLKK
                     NGFIPSHLVNRYEELVNKICILYNELIQDEPSIRIHGDCHKGNILKGENGFFFLDFDD
                     FVSGPAVQDFWMMLPTDKREKDIAREALITGYREFRDFNEESLRLIEVLRALRYVHYS
                     AWIAIRWEDPSFPNAFPHFGTNEYWEKEIKDLEIQLVLINRGESEDVEIVLADNEDIP
                     LTNKDFFWDLE"
     gene            20898..21608
                     /locus_tag="PLF66_00720"
     CDS             20898..21608
                     /locus_tag="PLF66_00720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62582.1"
                     /translation="MKKLFLLLLFNFIHCTTVGFHDDSLRKSIDFGEEQPLYVCVLHE
                     DTVRKEEVLELEKDWAKELALYKINLKFKRIQQVERFAFYTIDVLEGLRYYKVGPECE
                     RIVYMVGRTWGDIAYEVLTLGIFYGIGVKLEVHGVVETYTNTRGVIKAKYVALLQFLF
                     TSPSSTIVHEGYHLMGCPHMLWKDDCYKIIQEAKQNNLKNTRPDKIFSVQSTGNKSNF
                     YSSEQVNYFWSSNKEIPK"
     gene            complement(21615..22901)
                     /locus_tag="PLF66_00725"
     CDS             complement(21615..22901)
                     /locus_tag="PLF66_00725"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HMZ62583.1"
                     /translation="MNPQNLIILLLAFILTQISPISAQANKKKTKADKDKKIITETIK
                     NIDSKKEDDMRDADDDDKPDSSEPQPKEKSFFKVIAKNGTPLRDKPKLNGKKLLVIPE
                     EVIGEILEETKERESIENRIGNWLKVEYNGKTGWIFSGATIVKEDREDFLPQTLIGYF
                     LIKAAEPIFYKKPGRDILGAMPDYPEKGEVVPVYRKKIYFESEYYYFELKSLKPASED
                     IVVGWIGAKGGSFISPEAFSAYTLKNRKKKLKSFEKEMVDEILKGQPDEKQAVNYGKV
                     TTETIHFKDNGKKKKFYIISYPTGTKTKKGFGNYSVTYYLVWRDGETIHSLLAGDKKN
                     LKVIDIDKDEIPEIWAQFETIGEPPLCHIYTYQKGMFEQLFPNYVYCDTLQILPNGNI
                     QIQKEKEKIIYKYSNGSIEKVVTETSVKPNIPPNKK"
     gene            complement(22918..23781)
                     /gene="mtnP"
                     /locus_tag="PLF66_00730"
     CDS             complement(22918..23781)
                     /gene="mtnP"
                     /locus_tag="PLF66_00730"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003004062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HMZ62584.1"
                     /translation="MKDKVRAAIIGGTGLYELEGMEVIENYFPDTAWGKPSDTITIGR
                     YREKLIAFLPRHGRGHFISPSEIPNLANIAALKMIGVEEIVAFSSVGSLREEIAPRDF
                     VLPDQIIDRTKGIRESTFFGNGVVAHASFGDPFSKNLSERIYSLCEKLQIKTHKNKTL
                     ICMEGPLFSTRAESKMYRMIGGDVINMSVLPEAKLAREAEIAYQMVCMSTDYDSWKEH
                     EEPVTVEMVIGHLTHNNATAKRLIAELIPILGNGDDLSLVGTSKFSIFTNPTKRNETQ
                     MKKIRTLFPEI"
     gene            23888..24625
                     /locus_tag="PLF66_00735"
     CDS             23888..24625
                     /locus_tag="PLF66_00735"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HMZ62585.1"
                     /translation="MKIILFFLLWVLSTISVFSEDRKFLFRDQIVQSSDAFSMLGDSR
                     TQYVFELGAEVDWEDKQYLGKYKTSCSESKAGIQNAGVAGSTTEHWLEFLKSENYKPE
                     DFHQNIVLMIGGNDILRYSYKWRGFTFNKTDALNNALDEIHSRVVEIVNILRQSNKKV
                     IIQTHFRVNPAIKEGNSKSLNEGIDGLNLRLLETYGKDLDGDVTVAYIPPEFPAALFL
                     DLVHLNPFGYQLHSYFLARELERRCWW"
     gene            24727..25479
                     /locus_tag="PLF66_00740"
     CDS             24727..25479
                     /locus_tag="PLF66_00740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62586.1"
                     /translation="MIQFEVISRFMLFFRITLVCLFFLQACHSTTEKEHPIEDTHIFY
                     SNYKNSKGVVIHKNIKIQTRLIDVTQELESLEKIEIQEWISKIDKALSTVNQNIEHLN
                     VDGWKYITPKGILILAFTAAVVIPIPGTFETLTTIGFITMGVQRIREKEEDKQNRIRL
                     SDLLSECSEANDKLTHLRKTTLPDYLKKWSKIFYNKDNHVRNANDPADIRVYDVLWNR
                     YKIEKENLKNTIDLELEHANNQCYFVERSKDF"
     gene            complement(25476..25877)
                     /locus_tag="PLF66_00745"
     CDS             complement(25476..25877)
                     /locus_tag="PLF66_00745"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008595760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HMZ62587.1"
                     /translation="MFQKEKGKFYIRIEGRFESSHYLYKYYADGSDEAIHGHSWKVEI
                     YLAGKENIRPDGISFDFLTAKQKLAQLIQSIDHILINDHSDFKGINPTSENIARWFYS
                     GIKEEVKKAKGKVLKIVIHEGPENLAFFEPA"
     gene            26724..26915
                     /locus_tag="PLF66_00750"
     CDS             26724..26915
                     /locus_tag="PLF66_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008593840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide oxidoreductase"
                     /protein_id="HMZ62588.1"
                     /translation="MTVGEAMQVHPEAGLVFSSYHLGGCSHCSINEIETIEQVCQGYG
                     VEVDVLLDSLNNLFAEDEA"
     gene            26973..28016
                     /locus_tag="PLF66_00755"
     CDS             26973..28016
                     /locus_tag="PLF66_00755"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HMZ62589.1"
                     /translation="MLEKNDIQLGISPKPINETEERQLSLLLNLIRNRQGLSFFQIRQ
                     IMKEYYSNPNLESDQKKLHRDIDELRENGFAIKFFKINHDSQEINVYRMDDSQRDARI
                     QMTEEELRKFSFLILKKSEDGASNDLFSAAQKIFSRSLQHFPYANKKIKLDEPLEKEE
                     DQAVSDALYKIMSAIKNKTPLRIQYFKTNPSESYYRDIDPLQIIKRNSEDFYLIVYDR
                     EKKQKRRFLIPKILKVIEQSGEFLFSGKVTEDDLNYHALNFNIHEKEELIVTCNESDS
                     WKLEKFLFPHPFRKVDNRFYFSTTNRAALFNFILRENDCIQEVNSEIFVNELKQYLSE
                     LKNQYLKADSIQI"
     gene            28093..29379
                     /locus_tag="PLF66_00760"
     CDS             28093..29379
                     /locus_tag="PLF66_00760"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HMZ62590.1"
                     /translation="MITTKELIQDREIKGLRFISYFRLFFVFSNVGFTLVVGKSVMER
                     VVVGIISLLSILFLVYFHFLLKEKRKLGFVGATSALFDTIILIITPYIWHQSVGGDLV
                     SRAYILKSPSFIGMCYVILIIHGFFAIRPLHPLIISFGVSLGWVSFLVFALQDPRVVI
                     SSDFVSYMLGDTISMEFFITFLFSFSLSGLFLSFYTYQSRKLIFEAVNLEKAKMQVSR
                     YFSPNVFNKITTTEENLLTALARNQKVAVMFTDIRDFTSISESLSPEEVVQLLKEYHA
                     RMVNIIFQFGGTLDKFIGDGIMATFGTPEAQPDDAYRAVLAGIEMKRALAELNLQRSK
                     KNLPIIRQGIGIHFGNVIAGNIGTENRMEYTVIGDTVNLASRIESKCKELSTDFLISD
                     AVNSELQGRIPTNFKGEHTVKGKSISVSLYEVQVEV"
     gene            29531..29689
                     /locus_tag="PLF66_00765"
     CDS             29531..29689
                     /locus_tag="PLF66_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015682509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62591.1"
                     /translation="MSEENKEKKNKKISKMSSAELEAALKKAKDNNNSDISKYVQHIN
                     QRKAELKK"
     gene            29727..31634
                     /locus_tag="PLF66_00770"
     CDS             29727..31634
                     /locus_tag="PLF66_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004486419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HMZ62592.1"
                     /translation="MIQFINIRHQYGANVLYDGFSWHIKPNTKIGLVGPNGVGKTTLF
                     QMAVGLLKPDEGEIVRAKDTVISLFHQIPDFNGEKSVIETALTSNTLYKKYLERKEEI
                     DKRFETTDHESDAFEKLLSDQAELEEYAHTHDLHNLEVKAKKILSGLGFKDDAYLRQV
                     KNFSPGYHHRIGLAIALLNPYNLLLLDEPTNHLDDAAKEWLRDFLQTIKTTFVIVTHD
                     PEFLNEVTDTIAEISLKGVIEFKGTLEAFLEEKNELHEKLKNQYRKEEAYLKKRMEWI
                     DRFRAQATKARQVQSAIKKLEKRDAVENPEEIFWNKKPAYRFNHIPDGKITFRLENAS
                     FQYDKDGRVIFKNADLEVSAGEKIALVGPNGAGKSTLMRAILGRHTLSSGSIYFGPKT
                     RIAYFSQTHGEDLYPELNMIQSILKVYPDMSDEAVRTILGHFSFSGDTVYKQVSAMSG
                     GEQSRLRLALMVLTPANCLFMDEPTNHLDMVTRNALKHALMEFPGSLFIISHDPDFLK
                     GLCNRTFELSAGVLKNLNCSFEDYLKFHKEGVYGEEDQSVKPVKTKETNASRNNDKNR
                     IKKIQKEINDIESRLELLERNKKHLEEVLSDPAFFKNRSYQNELDNYNDTKKEIAFLT
                     ERWEILSEEIN"
     gene            31640..31975
                     /locus_tag="PLF66_00775"
     CDS             31640..31975
                     /locus_tag="PLF66_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004458900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HMZ62593.1"
                     /translation="MSPTELKKKLDEREKGNDDFILVDVRNPNEQEIAVIPGTDMLIP
                     LPDFDVEISKLEKYKSEGKEIIVYCRSGGRSTTAYGKLKRNGFTNVSNLEGGILSYSD
                     QADPSIPKY"
     gene            32029..32784
                     /locus_tag="PLF66_00780"
     CDS             32029..32784
                     /locus_tag="PLF66_00780"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HMZ62594.1"
                     /translation="MQLVLLIETDTQSLRDYAKYLSGNRFRLVDTDNAKKGLEYAKSA
                     PPDLLLLGLNPQDKDSIETLIYLKKDPITKHIPVLALISFFNESFLEQLKKIGISDHL
                     VKPFKQHTLVPKVNELLNLSEKIRANIVKHTTHHISITLNTDDRVVIVFRSGIKNFVL
                     PEIRNVLNHDFIKSSINKHIAIDIHTVHEISTEELQILEKITKLFGEKKISLITGSHL
                     GNIMKNSELPEFVNLFLSMHDYELFLENPDLDE"
     gene            complement(33263..34561)
                     /locus_tag="PLF66_00785"
     CDS             complement(33263..34561)
                     /locus_tag="PLF66_00785"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HMZ62595.1"
                     /translation="MRRNIVHPGADELIYEIRQIVGVGKQLEKLGVNITWENIGDPIQ
                     KGEAVAPWIKEIVSNLVQKDISWAYTATQGAENTRKFLAQKVNERGGVQITPDDILFF
                     NGLGDAVAKIFGFMRREARILGPSPAYSTYSSAEAAHSGYEHLTYELNPNNNWMPDIS
                     DIENKVKYNDSIAGILLINPDNPTGAVYNKEIMREIVNICEKYDCILICDETYAHVNY
                     SGSGSIHLSEVIGNKVCGMALRSISKELPWPGSRCGWIEVFNRKNDPLFERYIKTLLD
                     AKMLEVCSTTLPQMAIPEIYASPNFIPHLNERNQKYKERAKKAVSILAGTKGIKVVEP
                     KGGFFLTVLFEDGILNDKMKLTITNKSAEEFIQPLLRNIANDRRFVLNLLAATGICVV
                     PISSFCCKKDGFRITLLEEDPAKFEWVFTTIRDSIKAYLG"
     gene            complement(34794..35423)
                     /locus_tag="PLF66_00790"
     CDS             complement(34794..35423)
                     /locus_tag="PLF66_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003008048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HMZ62596.1"
                     /translation="MELLKNMIDFVLHIDRHLDTITTEYGTLTYVILFIIIFAETGLV
                     VTPFLPGDSLLFAIGAIAARGTLDVFLLFFILAIAAILGDTVNYWIGKFLEPKLETIH
                     FINKDHLKKTQDFYDKYGGKTIIIGRFIPIVRTFAPFVAGIGTMNYSKFILYNIVGGL
                     LWISIFLVAGYFVGNHPEVRKNFTYVIFGIIIISIMPAVIEYFRNRKQA"
     gene            35538..36269
                     /locus_tag="PLF66_00795"
     CDS             35538..36269
                     /locus_tag="PLF66_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002761924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HMZ62597.1"
                     /translation="MSKKVIVVGASSGIGAEIAKELLKSGAYVTLIARREKELNQITS
                     EIPNSKYCIIKHDVINFSEAPKLFQDSLKAMEGLDEIYYCAGILEKVAVDEFSTEKDI
                     RMLNINTLGAFAWLNLAADYFQKKNSGKIIGISSVAGDRGRIGSPAYNTSKAALNTYL
                     ESLRNRLARKGIQVLTVKPGFIDTDMTKGMKGLFWLISANEAARIIINAAANGKEDIY
                     VPARWALVGLIIRMIPSFIFKKLNI"
     gene            36389..37813
                     /locus_tag="PLF66_00800"
     CDS             36389..37813
                     /locus_tag="PLF66_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004500385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HMZ62598.1"
                     /translation="MNENTAIYFPISNPVKVETWGMNHFSMSRVYRPKTEREIRDLFA
                     YAQANRTKVVFRGGGCSYGDASVNNAGLVADMRDFNKIISFDEATGILTAQSGVTIKQ
                     LWEYSIERGFWPPVVSGTMMPTLGGALAMNIHGKNNFAVGPIGEHIVEFTFLTPTGEL
                     IRCNKESNKDLFYIFISSLGMLGCFIEIKIKMKKIYSGKMKVTPIPVKNLKEMIDVFV
                     KNIPDSDYLVGWIDAFGSGDGLGRGLIHRADNLKQGEDKDFPENCKLDKQNLPSTFFG
                     VIPKSWMWIFMYPFSNALGMRLVNFAKYLLGFVSKKPYMQGHAEYAFLLDYVPNWKFI
                     YKPGAMIQYQIFIPEENALSGMTEVLSQCQKRGLVSYLAVFKRHRKDDFLFTHSVNGY
                     SLAMDFPLTKGNREKLWKLCYELDEIVIRNKGRFYFAKDSTLRPEVAEKFLSKDAIQK
                     FKKLKEKFDPNFILQSDLYKRVFG"
     gene            37850..38191
                     /locus_tag="PLF66_00805"
     CDS             37850..38191
                     /locus_tag="PLF66_00805"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HMZ62599.1"
                     /translation="MDINTSSYNPAEDEIVVRFLGELKKSIPQYLKKVILYGSRARGD
                     FSKNSDYDFLVILSQKNLQLSDLVYDAGYSILDSYEKLASCIIWDEKEWNQKKDFSFA
                     KNISRDGISLL"
     gene            38571..38873
                     /locus_tag="PLF66_00810"
     CDS             38571..38873
                     /locus_tag="PLF66_00810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62600.1"
                     /translation="MFLCIPTSTALVTLLTAVIPTFRTQEGWKRYSEIECKDDYFVIM
                     YEGWERFEQSGSTFFLMSILNYPFDYDISQNEKFANFQKDFLRNNKIQTDSRGSEY"
     gene            39010..39291
                     /locus_tag="PLF66_00815"
     CDS             39010..39291
                     /locus_tag="PLF66_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010570611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HMZ62601.1"
                     /translation="MIESFRDKETEKVWNQEFSKKLDPQIQSVALRKLFMVARAKNIE
                     DLMIPPSNKLEKLKGDRKEQYSIRINGQYRICFFWKNGNAYNVEIVDYH"
     gene            39300..39602
                     /locus_tag="PLF66_00820"
     CDS             39300..39602
                     /locus_tag="PLF66_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015679763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HMZ62602.1"
                     /translation="MKKIPNIHPGEILSEEFLVPMGITAYRLAKETKINPTRVSEIIN
                     GKRGISADTALRFSKFFGNSVEFWMGIQDEYEIREERVKIAKQLSGIKSYKELVLT"
     gene            39659..39955
                     /locus_tag="PLF66_00825"
     CDS             39659..39955
                     /locus_tag="PLF66_00825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62603.1"
                     /translation="MFQGCISMVAIDQVNTFKNTTEHVQKENEVCNFEEVLNQKPINR
                     KRFYENRIILGTSTLDMGVSAFYYTMLNPKGFMLFVTGFYTYLSFGFLLLQFRR"
     gene            40075..40281
                     /locus_tag="PLF66_00830"
     CDS             40075..40281
                     /locus_tag="PLF66_00830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62604.1"
                     /translation="MNIIKAVLHYPVDYDIRQDRSFNEFKKTFLPNLTIRTYSRENVY
                     YRYIYYTGGKGKFEEDYAKYLYRQ"
     gene            40326..40682
                     /locus_tag="PLF66_00835"
     CDS             40326..40682
                     /locus_tag="PLF66_00835"
                     /inference="COORDINATES: protein motif:HMM:NF017496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HMZ62605.1"
                     /translation="MLAEILEDPLTFIHSEPEPDISIIRRSADDYKSSHPNTAEFVIE
                     VAVSSLRIARLKIEIYAESKIPEYWIVNVEEKNVEVYKTPEGRDYKEKKVYAVADTIH
                     FPQFLAGELPVAELFV"
     gene            complement(40801..41931)
                     /locus_tag="PLF66_00840"
     CDS             complement(40801..41931)
                     /locus_tag="PLF66_00840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL family lipase"
                     /protein_id="HMZ62606.1"
                     /translation="MKKTFFLLAIILFNLGLFEFILGMFDPEQILVKGFDKKLLFRMY
                     PDKTGKVVSEEYAVLVETNQNGFRQKIDSNHKYSTLLIGDSFTEGWGVEADEIYSEIL
                     NQSSKEEKFLNLGLHGSSPILFALQMKYYLETFKPKKVIVQLFDNDLDDNDKIEIFTE
                     LDKEGKVVSPKQRLGASIFGESIYNFFKERTLYRLTTKVVKFFQKQPSPILYYKVGRE
                     PKVKTLSHEESIQKFGKLSPLGKDINVKYGNQFGFYKDAEEELWKSRLRKNEIYLNQI
                     LDICKQNNVELSFLYIPAKEFFAKGGITGALKDFSSEAHSQKNPHYQQIQKVCKENQL
                     KCYFTNEYFFDKDPEKLYFPYDAHLNKEGHRVLAEMILEKGN"
     gene            41990..42919
                     /locus_tag="PLF66_00845"
     CDS             41990..42919
                     /locus_tag="PLF66_00845"
                     /inference="COORDINATES: protein motif:HMM:NF039905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HMZ62607.1"
                     /translation="MKRIVILNPKSRHGASRVAFEKMRPKLEDHLGFFDIYFTTAPKD
                     CTRKVREILHKKEYDQILIAGGDGSINEAVNGYFDHGHLIQSNIPMGVINLGTGGDFV
                     KTLNKRSQHYFNALRDNSYELVDCGVTTLEHGKEPHHFINITSIGMGGDMNRQMKASS
                     FQYGMAAYFYHTLSVLFKYTPAKCKIKMKQPNGNWLEIDSEVVNFFVCNAGFSGGGMV
                     WAPSASIEDGVFDIVLVSNVPKYKLITESHKVYSGKIASMTGVMEFKAVEIHVIPERT
                     LSQEIDGEVREMDFLQTHEFHFKLIPKAIPMVI"
     gene            42924..44408
                     /locus_tag="PLF66_00850"
     CDS             42924..44408
                     /locus_tag="PLF66_00850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62608.1"
                     /translation="MSLKKIFVSILVFYFLIFLILPHTEYQPADPGIKILQIKDFILS
                     GYSNFFASYLGRELDPSLTFFPTKPPFGYIVADNPYYVFPFFVTIAYTPFYLLGGIYA
                     LDLLSLISGLLIVFLLYKISNLLEMSETFQKSMILFLGFGSINSIYSFMFGESIHASL
                     FITFAFYLILKTRSTPNSFYFLIAGLISGLSVLFRIELAFFCFLFLLGIFIFQMYKSK
                     LDLILLCVGFGIPAFVLIIANYKVTGNILGLRGIEFLNYSGASYPIEKRLFNLVKALF
                     VSDKGLGLFTAWPVFLFLIPYFSKFKMWKEKKELNFILFVSIVFSLLIPLIVKNDDGS
                     ILGPRFASSVQPLLIVGTFYSIQLLADQSILSKWISKLSYFIYYSIAVTILGYIILFF
                     FLKASHKMNIEIDKKISGEVIFISKPDYFSLVFPSVYRKPILSVDSEEEFHKFCKNNR
                     TKIPNMISIVTSNPKYDISTELEYKLVDSFSSNGIHVYNFKSAN"
     gene            44417..45685
                     /locus_tag="PLF66_00855"
     CDS             44417..45685
                     /locus_tag="PLF66_00855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62609.1"
                     /translation="MKQFFKKLFQYFLLFLLILVFLRIIDLVFIFMGQEGKAIFYPPN
                     VVAHSDSKDYYYTFKTNSLGIRNPEIGDKKGKRILVLGDSFVFGWGVNEEFTFVRVAE
                     KKIQQVDPNISLINTGIPNTGPENYLPVYRALRDKIKPDIVLTVLYPNDVTDSGPTVF
                     FEAIKTALSKKKLIRFAFFVMPNASDYFLKYFLARLNQGINSDAESTSLSKQDNSQPP
                     PPKVLSPEQVLAKKEEYRKMIYLLAQNMDIKKDRVDLWLTEIGDENLTLAAKGKISPK
                     ILLTGLLSPDFYEENLDLKKKKIDRFARMLELVEKIRVEASERKEKFALVYIPSELQY
                     DPKKTDLHKKLGVTTKEDWLSKTSNLELELQKYTNQNQIAYLNLTETFRKESKQKELN
                     FPFDLHLNEAGNAVVVDPLVEFLKTIDKGN"
     gene            45687..47297
                     /locus_tag="PLF66_00860"
     CDS             45687..47297
                     /locus_tag="PLF66_00860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62610.1"
                     /translation="MNIQDKIKTSPIASIVLVGVVFFYLIFFSSSKGVQYGDFGLMYI
                     QVKDIVESGYSTFAVRYKGEKADPDHKYFPYTKPFIGKVGQKYYIDFPPYFPLLNTPF
                     YQYFGMNGIYILSYLALLGTLYLIYKIGALYELSFEMIHLSIFLYSFGMTATMYNLVF
                     HEYPIAIFLITSSFYFGALYYTRKNNYSLVWFGLLGGLSLFFRLEMIFVILSIGISLV
                     LTNPKDFFKIGFLSFLGFIFPFIALLSLNQYIHGHPLGLRYILTLTEDSSISLLGRID
                     IIRDMLFSSTRGFFFQSSFVIILPFILFLRKLILFQERFLFLLLFFGFGFILLTAPNH
                     GDHRAPRYLFGLYPILVLLVVISFSSYIKATSFEGKSVFQKPIQILFSILVLISFLAT
                     IQNYRWMQKATVNVISLNETISNLADSETAIIFRDYAQPLNTQKIYLERTVFVSEKLE
                     NLSELIHKIRESGFKKILVSQVYSAPLNEEAKEKIFRDYITSLSLSKISESSYEIGNE
                     TEKGFVSPRWIQFGKNQNLFILNLSFLK"
     gene            47539..48642
                     /gene="fliN"
                     /locus_tag="PLF66_00865"
     CDS             47539..48642
                     /gene="fliN"
                     /locus_tag="PLF66_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020783319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HMZ62611.1"
                     /translation="MGDGSLSQDEIDALLQGADETPSYETSGSSAGMGGAGDDLSPLD
                     RDALTEILTAAFSQAGAMLGTLLSKNVKFVNPTPESKSAADLSAEIGAGSVCLFSTLS
                     GPVNGRACLVMSMENGSKIAHLLMGGLSTGAMETAQLETLKQGFTSVIGNVTVQIGTK
                     VSAPITGSPVDVSSSKSSRDLILPDGKTLVRTTLNLNIDGIGVFKVHYIISKSMADEI
                     LALSRRTPNRPNYNDGGGMSVNVPANNFGNPVQGMGMSSPGPQIGIKGVSFPSLSTAG
                     APSGATNLNLLMDVQMSLTVELGRTKMYIKDILGLGEGSIIELDKLAGEPVDLLVNGK
                     LIAKGEVVVIDENFGVRVTDIVSPTDRIKNESK"
     gene            48721..49404
                     /gene="fliO"
                     /locus_tag="PLF66_00870"
     CDS             48721..49404
                     /gene="fliO"
                     /locus_tag="PLF66_00870"
                     /inference="COORDINATES: protein motif:HMM:TIGR03500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HMZ62612.1"
                     /translation="MQTFLIAGERDEMDQLLQKELGQEPTKQLTEDNKKPTDKKNEPI
                     NPVEERYRREDDSSSLLWTLVKVFFVFGILTAAMYYVLKFVSKNRQSMYPVRDAMRVL
                     ASLPLAPNKQIQIVDVSGILLVIGVSDGSVNLIKEIDSSEIKEKIYHSKETVQPPTEN
                     FLEVLLSTFKNLDFKASHNTSHKDTEVRDEEIMNEIKFRQLERLEKLKKERSDLSGGK
                     DKDISDSFS"
     gene            49471..50280
                     /gene="fliP"
                     /locus_tag="PLF66_00875"
     CDS             49471..50280
                     /gene="fliP"
                     /locus_tag="PLF66_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002747827.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HMZ62613.1"
                     /translation="MKQGKSLFTKRLVLRLASISILAVIISISSIEIYAQSTNTRIPI
                     PNLSFNVNEAKNPKETSLSLMILFLVTILSLAPAIIMSVTSFTKIVIVMDFVRRALAV
                     QNLPPNQVMVGLAIFMTFFIMAPTLGTINDKALTPYLDGKIETNTFFERSMVPLREFM
                     MRQIGKSGTKDVALFLKLGKVKQVKSFEDVPSYVLIPAFMLSELKKAFIIGIYLFIPF
                     IIIDIVVASTLLSMGLMMLPPVMISLPFKLILFVLVDGWNLLVFELVRSYK"
     gene            50277..50540
                     /gene="fliQ"
                     /locus_tag="PLF66_00880"
     CDS             50277..50540
                     /gene="fliQ"
                     /locus_tag="PLF66_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012389491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HMZ62614.1"
                     /translation="MTEVDVVSLIREALFITIKISAPILVTALIVGLILGIIQTTTSI
                     QEPTIAFVPRLIAIFIIIIIFASWMVRTMTDYTRNIFMMIERI"
     gene            50540..51322
                     /gene="fliR"
                     /locus_tag="PLF66_00885"
     CDS             50540..51322
                     /gene="fliR"
                     /locus_tag="PLF66_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004464965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HMZ62615.1"
                     /translation="METFVNNFQTFMMIFARIMGLFSLAPIYSSEAVTFQIRVIIAFL
                     LSMILFPVTANYLHAVPTGMGEYALIIASEALIGVLIGFIISIIFAGFQMAGEYFSVQ
                     MGFGYTEVLDPVSQTSLPVISTLKNLFGMLVFLTVGAHRTMLESVAFSFQKIQLLTFT
                     REVNIGLLKAFEYAIGAMFLVAFKISLPVLGILILVTIAEAIMGKAAPQMNIMQLSFP
                     AKIFVGLMVLIIIIPFIDKQMEASFTLTFDRLNTLMSEWPQK"
     gene            51373..52455
                     /locus_tag="PLF66_00890"
                     /pseudo
     CDS             51373..52455
                     /locus_tag="PLF66_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000709366.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
     gene            52455..54569
                     /gene="flhA"
                     /locus_tag="PLF66_00895"
     CDS             52455..54569
                     /gene="flhA"
                     /locus_tag="PLF66_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020779067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HMZ62616.1"
                     /translation="MSEKKWYNQSDVVMGVGVIAIVAMLIIPLPGFILDLLIVVSLAA
                     GLMILLTSLSVKEASDFSIFPNLLLISTLFRLALNVSTTRQILSQGASFNSHIIEAFG
                     TFVVGGGTGLGKYVVGFIIFLILTIVQILVITKGATRISEVAARFTLDALPGKQMAID
                     TELSSGNINEEEARKRRKKIQREVDFYGAMDGASKFVQGDVRAGLIITAINLLGGIII
                     GVSIRGESFVSAIETYGKFTIGDGLVSQIPGLLSTTATGMIVTRAGSESELAEEFKAQ
                     LFNNPKTLYVVSGSLGLASLIPGLPFFSLLLFSLCFAYLAYSIEQNAKESLAAIERKA
                     GESKAEKKPDYYKELRTDPIEVELGLNLVPLVDTNQGGVLLDQISNLRRRFAIDTGLV
                     IPAVRILDNLELHQDSYAIKIHGVVVGESKIRPDRLMALDNAKKVTEPVKGEDFIEPT
                     FGYKAKWIDPADKVDAENKGYTVVDASTVIVTHLKELISNHAATILGREEVKSLLEHQ
                     KTTHPTLISELEDKKVGIGLIQQVLQNLLKEGLGIRNLSTILESIANNESRSRDPFFL
                     SENVRQAISKQIVIDYLSSDGKLHVITLDPRVTDRLSKGIVVDPIEGSLISIPPDYYN
                     KLIEAAAREYNRCHSEGKFPIFVVSRPIRMPLSYMFAKEFPPRNFAVLAIEEIQSSTK
                     TVMDGVINVQSNEGVRVATNRG"
     gene            54638..55915
                     /gene="flhF"
                     /locus_tag="PLF66_00900"
     CDS             54638..55915
                     /gene="flhF"
                     /locus_tag="PLF66_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016547780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HMZ62617.1"
                     /translation="MDFVKIKGKDIQDCFMQMKMKYGPEAHVYNQRVVIEGGIMGTKL
                     LSKKFYEIEIGIPEQQTSKDRVEKKLQDLKELLRQKSEDDTRKKSIHDLKPLLQDEKS
                     YSVFKSLEKEEDAIVDTNQNQSKNLGLSIKDEITKKDSGKNNANESTYLKRLKERLIS
                     EGMTLDFLQELIIKTDKHLSSIDKEKPSAVNDKFAGILEDRISVDSDLFTGTSRGKRK
                     VIFFIGPTGSGKTTTVAKLAAKYFLHMGRMVSLYTTDNYKIAAIEQLKRYADTMDIPF
                     YPVKDVKRLQEQLLRDGSELILVDTPGYNHKNQDFANRMKQYKDAFSEKDQIENILIL
                     PATSSYSNMKSVIAAYESIGFKRIILTKIDEADYVSPVLELADLNNKSFAYFSLGQEV
                     PFDIVAASKKTLSEIVVYPDKIKDLKGDTVATG"
     gene            55934..56809
                     /locus_tag="PLF66_00905"
     CDS             55934..56809
                     /locus_tag="PLF66_00905"
                     /inference="COORDINATES: protein motif:HMM:NF012369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HMZ62618.1"
                     /translation="MQPNQDVLPNDEFPAQESDKRGSKIIAISSGKGGVGKTVFTVNL
                     AVSLAKAGKKVLVIDGDLGLGNVNVLFGFIPKFNLYHVMKGHRSIRDIVFHTPEGVDV
                     IPGSNGYPQFANLDDKSRDQLIQSFSQLDAYDYVLVDTGAGIGPNVIGFTLYADEVIL
                     VVAPDPSSVTDAYGLIKSLISLDKKKKISFIVNKIRSEAEGKKVVKRITEISNKFLGI
                     KPISIGFLYQDEDVDRSVRKQKPFLLFAPRCKAAENLYEITGTIANIPIDPYQTQPKM
                     MVYFKKFLSSLIPNT"
     gene            56903..57298
                     /locus_tag="PLF66_00910"
     CDS             56903..57298
                     /locus_tag="PLF66_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002556459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62619.1"
                     /translation="MHIVFVSGIGSLIFGGLGLGIYMVLENKVPEFLDFLAEVGGGIG
                     FRRAASSDPMGMGGNYSSDNEIKLTKSDESMSSDFGGGDISSGKTKDGKFGDHIIINN
                     IPIKNEPKLMAEAIRTIMSQDDGETPKQA"
     gene            complement(57406..58881)
                     /locus_tag="PLF66_00915"
     CDS             complement(57406..58881)
                     /locus_tag="PLF66_00915"
                     /inference="COORDINATES: protein motif:HMM:NF021778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HMZ62620.1"
                     /translation="MKQDLNKKITSVGICILIFASINCLKAKKSPYDIGNTFRFFPAI
                     NLIQGLINSNLSQNTPRNPPASVNFIDTDILNFGKIGGTISVTKAVDESDIEKYNLYF
                     GTSGTSKYNSTILNSLPKTGADLSFQLSDNTTIPSGVTHILAYSSNSSGENPTPVSVL
                     IKNFEKSGKYLYVYDSSANLKYFSISAAGVLNPITSYVVGSGTVRSVSIDSSGKFLFC
                     AAGNAILPRIINSTDGSLQTATFTNYISIQGATVDSVGSFLYASASNAGSFELSSFSI
                     NPSTAILTLVQNSSFNVGVTVTSSIFLDRTGKFAFVLRSSGDSNLYSYALNSSNGQIL
                     SSVQNTGLPLSQNFMVSHPTLDIVYLATGTSIYAFSVNQTTGALGSLGFFAVAPSGLR
                     GMAIEKSGKWLYAVSSTANTAYKVDLNSSGILTSSSTVVTGLNSPASVISDPSGRFLF
                     IGDSINTRSYQINADGILSLISTIAGSTATGSGMVIASYIE"
     gene            complement(59287..62520)
                     /locus_tag="PLF66_00920"
     CDS             complement(59287..62520)
                     /locus_tag="PLF66_00920"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HMZ62621.1"
                     /translation="MRIALYILMIVSLVSCQKGNTRKSPKAIKGVLDLRSLPDGKKGW
                     NFSEDGVVNLQGEWEFYWMEFLEPGNPEIEKNISPEQKKYINKPGVWNNFQVDGKPIG
                     STGFTTYRLTVLLPSAEESKRNTLAIRLSTASSAFRFYVDGKLLTEVGKIGKSAEEAN
                     PEFRPHIIVLPDLKDSVEFVFHVSNYSHDKGGLWGFTWFGTIQDLQRMRENSISLDLF
                     IFGALFLMGFYHLGLFLLRTKDSTTLLFGLFCLFISIRSLMTGEKIWVQLFPGFSWFL
                     AIKIEYLILYLGVPIIIEFVAKVFPSEIPNWLRKGIILSPLLLSLTIILFEPIVFTKY
                     LPFMHVVIVIALLTSIYTLSLAVYRKRFGAITFVFGFFVFTITIVNDLLFSNGVIRTG
                     HFAPFGFLFFVFSQAFFLSKRFTNAFTQVETLSQSMESQNRKLEEQNHKLQDLDRQKD
                     EFLANTSHELRTPLNGIIGLAESLVDGVAGEQSKEAKANLNLIISSGRRLSNLINDIL
                     DFSKLKHKELQVNLQPVDIYSITNLVIQLSEPLVKKKNLKLINQIPESTTAVLADENR
                     LQQILHNVIGNAIKFTEEGSVTVSANLKENSQVLEIQIADTGIGIPKDKLEKVFESFE
                     QVDASNTRKYGGTGLGLSVTKQLVELQGGNIWVESELGKGSKFYFTIPVTDSMPHKIE
                     KSTMTPIHQSLEEGNAIEENESKTKANAHILVVDDEPVNLQVISNHFSLRGYKVTTMV
                     GGKEALEYLEKEKPDLVLLDVMMPNMTGFEVCEKIREKYDMNQLPVLFLTAKNNASDL
                     LEGFEAEGNDYVVKPFSKVELLSRVETHIKLKDKTNEIALVSKVKDEFLSNLSHEIKT
                     PLSVIYAYAEMLNNGKDFPDKIKKYSNKIYDSAQKLNEYVSDLILISDIESNLQLQNS
                     DLDSVELINRVVNTYKPLMAEKEINLKLNLPNKMNLSGDSILLEKAISAVMKNAIVYN
                     KSGGSIEISIGQSSLRLRSGSESAGIQIQITDTGIGIAKEHQEKVFEKFFRVDSSLTY
                     EVSGVGVGLFIARKIIELHNGKIVLESEIEKGTTVLIQIPISESNEK"
     gene            complement(62511..64646)
                     /locus_tag="PLF66_00925"
     CDS             complement(62511..64646)
                     /locus_tag="PLF66_00925"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HMZ62622.1"
                     /translation="MKIKNQSILKNKTVYGKIFIFLIAWFTFYSSIYAIEPVVLTEDK
                     GEYPLGLYLEILEDKEGKLTIEDVQKPEMEKLWVRSDKEVPSFGFTKSVFWLRFKIEN
                     KLKSKEDWLLDIAFPPIDYIDYYHMEDNGKYTLIQTGDRRLFHSRQYDNKNYLFKVEV
                     NPNNSRFIYLKTWSFDGLHESIPLTLYNKDTYIKTSSINDTFFGIYVGIISVMMFYNF
                     FIFLSVRDKTYLYYILYLFFYLFWVFIYYGYAYKYLWQDNSWWANQSITLTNILFVIM
                     LVLFTRSFLSSRDLIPRLDFILRLILFLYSSYLVLSFVIGYAAIWRIIMLTQFILCII
                     ILFAAIQCLRKGSRTAKYYLLAWSFLISGGMLLLLKISSIIPSNFFTEKSFILGCSFE
                     VILLSLGLADRINELKKKNEIIQIENQKELEKLNNELEKKVLVRTEQLNEMLDQLGKM
                     YVDSEAKRKEIEALAESRKKLSFVGTMAAGIVHDIKNPMATIKALAEMSNSETITPKK
                     REKNLNIIIREMDRLNDLAYEILDFSKDELHLNLEDVNIKDFILEIEQFLSIDFKYSG
                     IDYSSEIEYEGFAQIDKGRMRRVIVNLAKNAIEAMQDGSKKYKFKIRVESIPQNPPLG
                     NAGVSAREGLTISLTDNGSGIPESVQTRIFQAFATEGKANGTGLGLFMCKMIVEAHNG
                     RLTYETKLGEGTSFFITLPEFIGEEIVCG"
     gene            complement(64766..65323)
                     /locus_tag="PLF66_00930"
     CDS             complement(64766..65323)
                     /locus_tag="PLF66_00930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62623.1"
                     /translation="MKNKKLEIVFQPNLEDTIDANLRLTGKLESTKKKLRESKNLGLF
                     SLVTSLVAIIYLIYENNFKSKSLFYLYLFGIFILFLNFLYSFFTPWIWKRRVSNFIKE
                     EFIQDGETRISLNKNGIESSSKSITVNYLWEEIGEVNREQDLIELYFTKKGIIILREK
                     YFPNKESYNEFLNQLKELLPESKKF"
     gene            complement(65612..66847)
                     /locus_tag="PLF66_00935"
     CDS             complement(65612..66847)
                     /locus_tag="PLF66_00935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62624.1"
                     /translation="MIIAGIYQLLSNSNQFTLIQKKFLLLQQIINKIETFLNRISSYN
                     AYKYFFYPSNFTITTILFSLILSFSCSTTPKNIKLPEPVFSKDCTHNKYFIKDLCNDE
                     LKYLDWAENYQDYPSVKDLTNEKLRLVSNKYGIDEAAALLYSRILRDPKNLNLYQYIE
                     KQKPLLEKKIPDLSQKNLSLVFIPGLFYEKNKEFDSEGFALRKLAESLGYKTKMIRIK
                     QTGSVYENADIICTYLNQTTAEKVIFTTLSKGASDLKIAISLCGKEPYFQKVVGWFNI
                     VGLVKGSPLANSVEEHWYSSFFWRMYAKWNNYDYKGLLTIQTGPSTPLDIELNLPTQI
                     KLINVLAIPIRRTVSERAMPYYDEMAVLGPNDGMGVLADMYVPFGYTYASWRNDHYFF
                     QPMSEQLILSLLIFMAEGK"
     gene            complement(66860..67237)
                     /locus_tag="PLF66_00940"
     CDS             complement(66860..67237)
                     /locus_tag="PLF66_00940"
                     /inference="COORDINATES: protein motif:HMM:NF024546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRL domain-containing protein"
                     /protein_id="HMZ62625.1"
                     /translation="MKKNFLVVFVIALAMAISCANNYPTRLDPPGTYGMGGAPGSLFG
                     SSKGNVSCQNSNDSCIGQSVGSKTGEACASIFLYGLIAYGDMSVVAAARHGKVTKIGS
                     VDYSQLNVLGLLFLQKCTIVSGD"
     gene            complement(67805..68926)
                     /locus_tag="PLF66_00945"
     CDS             complement(67805..68926)
                     /locus_tag="PLF66_00945"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain -containing protein"
                     /protein_id="HMZ62626.1"
                     /translation="MEELENLQLTQITDEVKDKWQNIITIMANIINVPTGLIMRRIGA
                     DIEVYLSSKTNHNPYHPGDKEHFDNSGLYCETVIKTRKMLLVPNALSDEHWKDNPDVK
                     LNMISYLGFPIHLPDKTPIGTICVLDNKENQYSELFINLVSSFRDMIEKDLELIWKTH
                     NLHENNLKLNQLLNEKEILLKETQHRIKNNIANIEGLILLQAQSAKSFEVKIALQDVI
                     SRVQSIRILYEKLLLSNDIHEISMKDYIDSLINSLYDVFVDQDSIVIEKDITEFNIKA
                     NHAITIGIIMNELLTNIFKYAFKERNNGRIFISVNKNEKSITLTMQDNGIGFSNDQHQ
                     TKSTGFGLMLVKMLVEQLKGTYSVASKNGTTSIIEFALE"
     gene            complement(69064..69348)
                     /locus_tag="PLF66_00950"
     CDS             complement(69064..69348)
                     /locus_tag="PLF66_00950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62627.1"
                     /translation="MKFIEPSVQRAEVRMDPSQKEANTEYPQKIKNSTEDCFRLDQLT
                     GSKATEISGFFSLSNSQAILQFMNQSALNIIIFFFAKTQLFIYVQMYHFY"
     gene            69886..71916
                     /locus_tag="PLF66_00955"
     CDS             69886..71916
                     /locus_tag="PLF66_00955"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HMZ62628.1"
                     /translation="MLKLCYLILLFFYFPLFADSETPIFELKSSEFTTEPLPKYIYYL
                     EDTSSDLDIEKVQTPEIQEKFQAPKPDLSFGFKKNPYWFKWTVKNHVDRPIPWTLEIG
                     YPMLDHIELYIPEEDGKFTTIVEGDEKPFYERKVYYRNFLFELQEKPNSDRTYYIKII
                     TTSSLSFPIQAWTPRALTEKINTEQTLLGMSYGIIFIMIFYNLFILLSTRDRSYMYYI
                     LFVLFYGSFLTTLNGVAYEYLWPNQVWWANHCLPFFINGGSLFGLMFGRNFLNLKTSH
                     PKYSIWIRFLMILAFIGGILSLLASYSLAIKIATALAGVTVGTLLIAGILGVLKKYRP
                     ARYYLIAWFALILGIASYSLKAFGLLPTNFFTHWGVQIGAVMEMFLLSLALGDKINTL
                     KAEKEKAQKELNIAYGRFVPHEFLNFLEKKSILDVNLGDQVLKSMTILFCDIRSFTSL
                     SESMTPEENFNFINDYLGRVVPVFRKNNGIVDKFIGDGIMALFPGRPEDAVCAALEFL
                     NILRVYNQERISSNLTPIRVGIGIHCGPLMLGTIGHEEFMQGSVISDSVNLASRIEEL
                     TKKVGATVLISEGVLNELGDSREKYNIRYIGNIRVKGKVNNTKVYEIFDGDSEEIRNL
                     KLVTREDFGNGVEFFYKKEYQTAKEFFQKVVRKFPEDKATITYLSRLNEDTL"
     gene            71900..72085
                     /locus_tag="PLF66_00960"
     CDS             71900..72085
                     /locus_tag="PLF66_00960"
                     /inference="COORDINATES: protein motif:HMM:NF013595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62629.1"
                     /translation="MKIRFNFISSAYRIYFGSSGSSDGVGTSATFNNPYGVAVDSSGV
                     VYVADSGNNKIRKISCL"
     gene            complement(72178..73605)
                     /locus_tag="PLF66_00965"
     CDS             complement(72178..73605)
                     /locus_tag="PLF66_00965"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HMZ62630.1"
                     /translation="MTKIFFSILLIFLILQCKSPNQDLLKAVQTGNTSDAENAIQKGA
                     NPNTKYPELDNSSLLHISASVNDVEMLKLLLAKNCDVNSKNDLEMTPLHISAMTGNLE
                     VAKLLLENKANINEVDLRGFNVIHHVAHTENLELLKLFIDKGVNPKTTDNSGTSPLEY
                     SARQGNLILFENLLNYYPELTENEKEKIIRSAFWGDNVNIFKSLLVHKVDLGYRAKEN
                     YSLLHLASFNKNPAFTQFFIENGINVDITNDRQETPLHIAASQGNLEVVKVLIQNKAN
                     PSLKNSDQETALDLALEKQKEEVVEYLKSLNANKNNSESTQNDNNAILEFAKLGKLEK
                     LKNYLKKGSDINTKDENGATLLILSLENRKHEITEFLLSQDADVNPKKNNGENAIYIS
                     IPSAPLKIIQLLVEKGSDINSANLYQDTLVHRAAMYEKLDVLKFLVSKGAKTKVKNKF
                     GKSPLDYALDSKNKDVVAYLKKLEK"
     gene            complement(73851..74303)
                     /locus_tag="PLF66_00970"
     CDS             complement(73851..74303)
                     /locus_tag="PLF66_00970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62631.1"
                     /translation="MIKNNCPVCSILQNGWESGSLLESNFDLSVFKIINKSESGITYL
                     YQCPQCNTYFIRQVSQEWDSYNEKIIEIEKINPLNNDQYETFINPTIPDPPYREISNP
                     GHCSKCHSANIQKLREVKVGNNYLLWCKCLNCENEEFMDLYESSEFDE"
     gene            complement(74751..75950)
                     /locus_tag="PLF66_00975"
     CDS             complement(74751..75950)
                     /locus_tag="PLF66_00975"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HMZ62632.1"
                     /translation="MAFNINKSKEDLIKEIQSLKEENDFLKSVHEANKRQTTDSIKES
                     EDNFKIIFERSLSAMVIADDNGNYIAVNNAASELLGYSIQELLRMNVGELVTISNPSA
                     IERYAEYIRKGEEIGEFDCVSKDGIPKIVKYQAIRLKPNFNLSIMMDITEIKKNEQEQ
                     KINLQKLETLNKTKDKFFSIIAHDLLNPFIGIVRLSELIESNLKQNKQQDSSLMEYTR
                     IIRTSSQSGYELLENLMQWAKSQTDQIEYHPEPLSIHQLISKSVALIKGNAFNKNISI
                     EVIASKDEAVLADETLTSTVLRNLLTNAIKFTNSNGKITITTKQKDSFLEISIKDTGV
                     GIEKENLTKIFTVDSKFSSLGTNNETGTGLGLILCKEFVEMQGGKIYAESNLGIGSEF
                     SFTLPLS"
     gene            complement(76497..77660)
                     /locus_tag="PLF66_00980"
     CDS             complement(76497..77660)
                     /locus_tag="PLF66_00980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62633.1"
                     /translation="MKIDFRNGRVYQFNTLLASVIILTMPLLNCQKSHVDENKSAAAL
                     AAIVSQRNRTNQISTPSSSTANSYTIGGTITGLTASGLVLQNNAGDDLTIASGLTSFT
                     FVTKVTGAYAVTIKTQPVAVVCSVTAGSGIASADVSVAVNCVPSGSTWTAITLLPASS
                     WASVTFGNGIFVAVANATNTAATSTDGINWTTRNLPSSAQWWSVTYGNGVFVAVAMNV
                     TGAASSPDGITWTTRNLPSAASWSTVTYGNGLFVVASQGSSTAAASSSDGITWTARTL
                     PSSQSWTCITYGNGLFVALAFSSTIAATSPDGINWTTRSLPSSSDWKAITYGNGTFVA
                     VPTGPNSVAATSLDGITWTTRTLPSSQNWYSVVYGNSVFVTVAYTASVAATAP"
     gene            complement(78060..82709)
                     /locus_tag="PLF66_00985"
     CDS             complement(78060..82709)
                     /locus_tag="PLF66_00985"
                     /inference="COORDINATES: protein motif:HMM:NF015140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007154 - cell communication [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Calx-beta domain-containing protein"
                     /protein_id="HMZ62634.1"
                     /translation="MIPLPFSPSRDLAKNYVFENANAVFTNEYGRQASFTLKLSKKPE
                     ADVVIGSITSSNPAEGILLTSSSITFTPDNFDQPQTITLQGVADGIADGNQQYRVRFN
                     SVQTNDYSYSVLTIPDVLAINTDKETASIAASPLLGLSTNENGQTASIYVVLSTQPGA
                     DVVIPSFSSDTTSECAVSGSLTFTSKNWDTPQVVTVTGVDDYLLDGAKNCLISSSAST
                     SNDQVYVNKTMPVVTVVNVDNDTAGFTYVPITSAITTEAGGQAQFSIVLNTIPSGNIT
                     VTGITTSDPSEGTISPSNLSFGPANWNIPQIMTLTGQDDFMNDGSINYTVVFPNAVAI
                     NPLDAAYDNLALSSAGSFTNQDDDTRGFNIVALAPTTSISPISVTEGGSSQTFQVSLT
                     SSPCDTPSTPVSCNPASITINLTNNNTSEYTISPSSLTFTSLNWNVGQTVTVTAVDDF
                     VENPNLNFTLVLDPIISTTDYSGLDPADIPIQVINNDISGFTINPVGGVTVNENDPGG
                     VGLETTITVVLNSEPTGNVTLGPIISTDTLEIIIAPTLGGGNSAITNRTLVFTPTSGQ
                     AIVNSDSNIDGINDTSTGGWNVPQTIRVRAVVDGTSDGTQLRAVQFGARTTTDTKYAS
                     SVITPTPDVPATNVDSGVPNILLQSISAVSFNEDGTSTITAQVVLATLPTANVTISNI
                     VSSDITEGIVIPNGGGGSITNRTLVFTPTTNAAVTGTNTTTGGWNMPQTVTIQSVADG
                     FDDGNLTFKITFPGATGAAEYATKIPTSSNAAYNNLLGELTLTNIDNDTIGFTISAAN
                     PFPITEGAGAQTFNIRLNSDPCSTPAALVNCTSQAVSFTLTNNDPTQYTISPTSLTFN
                     AGTWNTAQTITVTPIDDNYDENTMDFTLVLNPITSSSDYGGLDPSDVTVRVNDNDTKA
                     INLTLTTGYSNVTSSAGGYTEYNMNLGSSPFPGNTVTVTVSVPTAAPQEGKILAADNV
                     TQLDSRSFTFTSANFSTNQTFRVRGITGAGTGSANFILTASAAETGTIPPSGFTALTY
                     NNYNGVTATRTITNYHIGAGKKIVLAGNTTTISEAGTTQALFWVLLKQAPTANVTVNF
                     GIDSTYPCTLPVGSPTTQFTISPSSLTITSANWNQITTGNQITISKVDDLVDDGDVSC
                     PLKITSTTSGDSYFNGLVPVTDFTQPSIIVTDNDTRGVLKQSQNPLVSGYLITTRSGA
                     SASLQYSLTSQPMSNVVLTFAATGGYASFSPTTLSFTPANYNIPQPLTVTGTAASPAT
                     DTNYTITATASSSETASGFANGGIYNSYVDNQNATNRELLYNLVPCTGNTVATCTAVN
                     ATGGTVAGTYTTTEAAGKRYFAINLRARPTTTVSIAVSSSNTAEGTVSPATLSFTNAN
                     WNTQQIVTITGVDDSIADGPIAYNINFGAMSGDTAFTATIPSLNATNSDNDTTGFILT
                     NPSNSGVLESVLGDSFTVRLNSQPTANVSIPLSVSVATLPDTGLTITSTNPLLFTPAN
                     WNIPQTVTFTWTGDGVSTPSTRNHSVILGVATSADANYAVNPPDYTISINNLGGL"
     gene            83534..84562
                     /locus_tag="PLF66_00990"
     CDS             83534..84562
                     /locus_tag="PLF66_00990"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HMZ62635.1"
                     /translation="MKNIIILFLFLYQCHKIQTQFNPSTVVLVSVGNVLSEEKKIKVG
                     DKVSFGQNLKVGKNSFCDLQIIDAESIITIRLQPETEFVLRTKITPNQNIIRPRIIKG
                     EAFFNIVKLSKKEEFTVVASAIKATVKGTQFYIEVDSNGKTKLAVNEGYVLLKPGVIE
                     LDDLPLEKIKHLANAQSIIESENQKELNVPTGHSVSVSNEMVKKTNQFIESIASAENP
                     NSSIANEISRQNYFTKEDIQKLNQEEEEEYKKVFLELPPLPTEYSSNPEKLRLAVLSR
                     IHSLSETISIRMESILQRRRETLITYDGRKIHGIVYSEKDFFNVITLNGIERIPVKEA
                     ESIILYYK"
     gene            84684..86807
                     /locus_tag="PLF66_00995"
     CDS             84684..86807
                     /locus_tag="PLF66_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004785672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP repeat protein"
                     /protein_id="HMZ62636.1"
                     /translation="MKSFIVILFSILFLQCEISKNPLAPNGPFGIFMLYYNLSNIKAW
                     KYTDTDLVHYNLNDPILPNSPVIPSSAVLNTFTISEPLPSGLNFNSSNGIISGIPTSS
                     LEEKEFRFTANLKPLPDRSENTSSLKLNVDYFPMTSGVNNPFSAEFPFPDDFTYSNYS
                     IEPNLPTGFTIDSKTGIISGPVQTETKNLGTYKIKATRNDGVNVVGSASLKVTEWVLE
                     AYLKAPNAEAGDNFGYSASIDGDTIVVGAYFEDSNQVTITNGTLAVNGTGATDSGAAY
                     VFRRTDGIWANEAYLKAPNAETSDNFGYSVSISVDTIVVSAHLEDSNQTTITNGTLTV
                     NGAGASNSGAAYVFYRTGNTWANEAYLKAANAGAGDEFGQSVSILGDTIVVGSRMEAS
                     NQTTITNGTTASPDNSASQAGAAYVFRRTGTTWANEAYLKAPNAGVGDNFGYSVSIDG
                     DTIVVGARNESSNQTTITNGTTASSDNSAPQAGAAYVFRRTGTTWVNEAYLKAPNAET
                     NDRFGLSVAISGNTIVVGAGNEDSLQTTITNGTLVQASDVSATDTGAAYVFRRTGTLW
                     SNEAYLKAPNAESTDVFGYSVSISGDTIVVGVLGEDSNQTTITNGLAQIGVNGAINSG
                     AANIFKRTGTTWANEAYLKAPNAEGSPLNGDLFGSSVSISRDTIVVGASYEDSLQTTI
                     TNGTLVHSSDVSAVNSGAAYVFRRK"
     gene            86885..89014
                     /locus_tag="PLF66_01000"
     CDS             86885..89014
                     /locus_tag="PLF66_01000"
                     /inference="COORDINATES: protein motif:HMM:NF025670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative Ig domain-containing protein"
                     /protein_id="HMZ62637.1"
                     /translation="MKSFIVILFSILFLHCEISKNPLAPNGPFGLFTFYYNLSKIGAW
                     SYSEPNPLYSKDLPIDANVPKTSPLAIVKSYSMKTNLPNGLSLNTTNGYITGTPLELG
                     SSEVEIFANNDFFKEQYPTKLKIIITGFSYSPTNSCVFGSEVNITPTFSENFNVNDYQ
                     FSISPNLPIGVTINTSTGVISGRCLEKRNSSYVVTATNQLNTKFIANFQLNVVAWENE
                     AYLKAPNADGVPGNGDEFGTSVAISGDTIVVGAVKEDSLQTTITNGTLVQGSDVSATD
                     TGAAYVFRKVGDVWINEAYLKAPNPDISDNFGNSVSISGDTIVVGASKEDSSETTIVN
                     GTTASADNSEGDSGAAYVFRRTGNTWINEAYLKGPSTDVNDGFGGSVSISGDTIVISA
                     PGDDNSPSGVVNGTYTSNSGGVQDSGGAYVFRRTGTTWINEAYLKAPNRFNDDVFGFS
                     AAIDLNTIVVGALWEDSNQTTITNGTTASADNSAMNSGAVYVYKRNGTTWVTEAYLKA
                     PNTEANDEFGRSVAISGDTIIVGATKEDSLQTTITNGTLDQASDGGAIDSGAVYVFKR
                     NGTTWTNEAYLKAPNAEGGDIFGTSVSIYGDTLVVGAISEDSNQTTITNGTLTSSNNS
                     ASASGAVYIFKRTGTTWINEAYLKTPNMDAGDTFGKSVSISEEICVVGANNEDSNQST
                     ITNGLATGGNNPNTNASQGAAYVFRRK"
     gene            89107..91248
                     /locus_tag="PLF66_01005"
     CDS             89107..91248
                     /locus_tag="PLF66_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015681647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP repeat protein"
                     /protein_id="HMZ62638.1"
                     /translation="MKSFIVILFSILFLQCEISKNPLAPNGPFGIFTLYYNLSNIKAW
                     KYTDTDLVHYNLNDPILPNSPVIPSSAVLNTFTISEPLPSGLNFNTSNGIISGTPLTS
                     FEEKEFRFTANLKPLPDRAENTSSLKMNIDYFAMSSGLNNPFSAEFPFPDDFTYSNYS
                     IEPNLPTGFFFDSKTGIISGPVQTETKNFGIYKVKATRKDGVNVVGSASLKVTEWVLE
                     AYLKAPNAEATDLFGGGGGVSIDRDTIVVGAGGDDSLQTTITNGTLVQASDQVSAINS
                     GAAYVFRRTGNTWVNEAYLKAPNAEGADNFAFIAISGNTIVVGANGEGSNQTTITNGT
                     LTSSNNLAVNSGSVYVFRRIEGIWVNEAYLKAPNTNTNYQFGQSVSISGDTIVVGSRM
                     EASNQTTITNGTTASPDNSASQAGAAYVFRRTGVTWVNEAYLKAPNAGAGDNFALPVA
                     ISGDTIVAGARLEDSNQTTITNGATASANNTAGDSGAAYVFRRTGTLWSNEAYLKAPN
                     SETNDRFGQSVAISGNTIVITAPNEDSLQTTITNGTIVQASDQASNTSVGAVHVFRRT
                     GTTWTNEAYLKAPNAETGDGFGNSVAIHKNTIVVGAPDEDSLQTTITNGTLVQSSDTA
                     SDLRVGAVYVFKRTGNTWINEAYLKAPNAEGGALGTGDNFGGRVSISGDTIVVGANSE
                     DSNQTTITNGSTVIDNNSATESGAVYVFRRK"
     gene            91252..93396
                     /locus_tag="PLF66_01010"
     CDS             91252..93396
                     /locus_tag="PLF66_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015681647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP repeat protein"
                     /protein_id="HMZ62639.1"
                     /translation="MMKTLVAILIVVFLFQCEIKKNPLSPNGPFGVFTFYYNLSNIKA
                     WKYTDTDLVHYNLNDPILPNSPVIPSSAVLNTFTISEPLPSGLNFNSSNGIISGTPTT
                     SLDEKEFRFTANLKPLPDRPDNTSSLKLNVDYFAMTSALNNPFSAEFPFPDDFTYSNY
                     SIEPSLPTGFSFDSKTGIISGPVQTETKNFGTYKVRATRNDGVNVVGSASLKVTEWVL
                     EAYLKAPNAEGVNPDGDSFGIVSINKDTIVIGAAFEDSLQTTITNGTLVQASDVGATN
                     SGAAYVFRRTGTTWVNEAYLKAPNSEGGDQFNNVRISGDIIVVGASFEDSNQTTITNG
                     TLTVNGTGASNSGAAYIFRRTRNTWANEAYLKAPNAGAGDEFGQSVSIDGDTIVVGAR
                     NESSNQTTITNGTTASSDNSAPQAGAAYVFRRTGNTWVNEAYLKAPNAGAGDDFGFPV
                     AISGDTVVVGARLEDSNQTTITNGTTASPDNSAADSGAAYVFRRTGTTWANEAYLKAP
                     NAEAGDRFGHSVSISGNTIVVGALFEDSNQTIITNGVAPIGVNGATDSGAAYVFRRTG
                     TTWVNEAYLKAPNAEANDQFGWSVSISGNTILVGAPAEDSQQTTITNGTLVQASDTSS
                     DLRVGAVYVFKRTGNTWVNEAYLKAPNAEGGAGGTGDNFGFQFTISISEDTIVVGARD
                     EDSNQTTITNGSVVIDNNGAWGSGAAYVFRRK"
     gene            93489..>95285
                     /locus_tag="PLF66_01015"
     CDS             93489..>95285
                     /locus_tag="PLF66_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015681647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP repeat protein"
                     /protein_id="HMZ62640.1"
                     /translation="MKSFIVILFSILFLQCEISKNPLAPNGPFGIFTLYYNLSNIKAW
                     KYTDTDLVHYNLNDPILPNSPVIPSSAVLNTFTISEPLPSGLNFNSSNGIISGTPVTS
                     LEEKEFRFTANLKPLPDRPENTSSLKLNVDYFPMTSGVNNPLSAEFPFPDDFTYSNYS
                     IEPSLPTGFSFDSKTGIISGPVQTETKNFGTYKVKATRNDGVNVVGSASLKVTEWVLE
                     AYLKAPNAEGVNPDGDFFGDVSIDKDTIVVSSRGEDSLQTTITNGTLVQTSDVGATNS
                     GAAYVFRRTGSSWANEAYLKAPNAELGDNFRAIAISGDTIVAGATGEDSLQATITNGT
                     LVQVADQASNTSVGAAYVFRRTGSTWAFEAYLKAPNAEGVAPNGDNFGDSVSISGDTI
                     VVGASLEDSLQTTITNGTLVQASDVSGTNSGAAYVFRRTGTTWVNEAYLKAPNAGGGD
                     NFGGLFNISISGDTIVIGARLEDSNQTTITNGSDVIDNNSASNSGAAYVFRRTGSTWA
                     NEAYLKAPNAETLDQFGRSVSIFKNTIAIGSILEDSLQTTITNGTLVQTSDVGATDSG
                     AAYVFRRTGTTWTNEAYLKAPNTIGDNQFGWVG"
BASE COUNT        30720 a        15553 c        16989 g        32023 t
ORIGIN      
        1 aaatgtaaag aaacgcaaga gatatgagat atgagatatg agatatgaga tatgagatat
       61 gtttttgtct taaaataatt accactataa ataaaaaata ttatatagaa aattacggct
      121 aaataaataa aaacccatgc atggtctgat ctcgccattg catacttgaa ataaataaaa
      181 ttgccaatca aaaaagatgg gaaaaagaat gggtaggcgg tcttctttct aaatgatacc
      241 cacaataaag aagttaaaaa aatataggaa aaaaaataaa cccactttat agatgtatga
      301 tcatccgctt gagtcatggc tgatgaatta gcaattgaaa actccaaata ccccctaaac
      361 atattatata aatctgttaa agaataattg aatattatcc tcactattaa agccaataaa
      421 aagaaagata caataggggt aacaatcaat aaagttcgat ttttttttct ataaaataaa
      481 tacagccaaa ttgggagtaa actcagtatc aaaaagagat acatagattt tataaaaact
      541 aaaacaaaca gtagagtaaa taaatggaga gattttacta ttctaagatt tttataggtt
      601 tctaaggcta ctaagtagca taataacata atacgatgat caataggaaa aaacaaaata
      661 ctcccaaatg cgataatatg gattaacaaa ttgggaaaaa aaggtttctt gttcagagat
      721 ttatttaaga agaaaaatat ggataaatta caatatgact caaagatgaa tgaggaaagg
      781 attgcatagc tatagactgt tggtataatt aaaaaaccaa gaggaccata tgtaaaaata
      841 ggtatacttc caattacagt atcgtcaaat gcaaagtagc tagttgccca catgtaagaa
      901 ggatcaagat caaagccaat ttccattcct tcaaaattga aaaataaaat tgaaataata
      961 aaaaagaata gaataccaaa ccaaagtaaa ataggtttac tcataatatg tgatccaccc
     1021 taaacaaaag taaatgctat tctttctctt ggttagattt agaaaagtaa taattcctgt
     1081 aacaattgca tttaatccta gcagctcgcc gatttttttc atttcagata aatcacttcc
     1141 aatataatga atggaaagat tgaagtttaa aaaagacttg cattgagatg attcctttct
     1201 taagatagaa ccattatgca aaatgcttat gttttaactg gtagcctaaa aagtcctact
     1261 ctaattgagt tagatgaatc tttatcacta tctttgcaga aggtaaggat taccatcgaa
     1321 ccattgcagt cagttttcaa taagaaaagc cttttgcttt ctctcaaaaa aattcaggct
     1381 aaacaagaaa aacgcaatta tacttctcct cgaaaagaag atgttgattt atatattgca
     1441 aaactaagag aagactggaa ttagatgaaa tattatttag atagttgcat tgtaatttat
     1501 atcgttgaaa aaatagcgga ttattgagat actctccttc aattgcttcc agaagatgca
     1561 cagctttttg tatctgattt aactcgaatg gagtgtagag ttaaaccgat tcaggataat
     1621 aacgctttgt tgctagaaga atacgattta ttttttagtg agtctgttga gattgttaat
     1681 ttaacaaatg aagttatcaa taaagcaaca gagattagag ccaagtattc ttttaaaact
     1741 ccagactcaa tccatttagc ttcggcgata ataaatgaat ccaatttctt tatcacaaat
     1801 gataaacgat taagttcatt taaagaaatt gaaattttaa aattataaat gaagtcctag
     1861 caactatgaa tgtgtttatc ttttctttct agcggctatc gctaatttgc atccaacttt
     1921 tcccatttct atattacgag tgaatggttt aaagcaaaaa aggattgcga gtctttcagg
     1981 aagagatggt ttgaagtgga cggagtaaaa ttacaaagtg attcttattc tttcctatac
     2041 taaaagcagt attttccgct ccattgcctg caccatacag taaatagcct tgtgacggac
     2101 tggaacaaga ccaagcgata gtcgctgtcg atgttgtgac acttcgaaca ttgtaaaaaa
     2161 gaagttttaa atcatctgac tttgcacctg acgatacggg ggttttatcg cagcggagga
     2221 ggaaaataag gaaaagaaag attaaatgca agataagagt cttctttgaa aaaaatgaaa
     2281 aactactttt ctcatctgag gattttttgg cttctctttc attgagcatc ctttattctt
     2341 tcttgtttgt ctcgttctcg cctaattcgc atccccattt ccatgtctac ttcttccaag
     2401 tctttttccc aaactttatt ctgttacata ggcacaggca actctacata taccgttgtt
     2461 gtatcatcat ctaataaatc gaacccgaaa atgccgacaa taaagtctaa gagttcgtga
     2521 ggattgaatc caaggcggat accgtagtat aacccaaagg atacttccat tggtagaacg
     2581 acgtaggagt cacgaataat tgtgtgatac ttcttacctc gataacataa caaaagtcct
     2641 ttgctagaat ctcgcttgga tgcaccataa agtaaaacat tcataaaagc aatgttcttt
     2701 ctataatttc gaaccattat gatagatctt ggctcatgat agttactagt agcgaaaaac
     2761 aaaaattgaa gttgaccgaa agttccatat ctactgtctc tgcaaggacc attttccgat
     2821 cctgccttta aaaaattatt tgattcggaa tcgttacttt gatacattcc gagatgtccg
     2881 aaccgtaaac ctactccgct gctgtctttt gctccataag caagaccaaa cataatcgaa
     2941 tctatatata ttccgccacc ataaatatta tcctcgaagc cgatgttaac tatatctata
     3001 aaatcgttcc ctcgatttat taaatatcct ttaatgctag aacactggaa ttgaagcaaa
     3061 cagagagata taatattgcc cagtaattac tatttctcaa atgttctatc atcaatgtta
     3121 cccccacgtg ctcgaaagcc tgcataagtt tcatagccga gtttagcccc aaattgcgaa
     3181 tcaattactg aaggctttct ttctagcggc tatcgcctaa tttgcatcca acttctccca
     3241 tttctatccg acaagtgaat ggcttaaagg tcatgaaggc attcctctag tattgaagaa
     3301 aaacctggct ttaaaaattg tcaataggaa tcaaaattga aagaaataaa caactgttag
     3361 ccgcagcagc ttttgtgcaa gagtgtcgaa attcgtatgt atgtgcgggc tttcaggaaa
     3421 aaactctaca ctgaagtgtt gaagaatgcc ttttacctat cgtatcacca aactgctctt
     3481 acagttaagg gtaaacttat cctagataac atgatttcgg tgtaagacat ttttttcttg
     3541 attacccccg aagttattta tcggggatat ctagaactat agattcccgc tatcgcttgg
     3601 cggttgcagc gatagcgggt ggaatttcgg gaatgacggt ctaaataatt tgtcaaatcc
     3661 tcatatcgaa ttcgctttaa atatatctcg ctgcctatct ttaggagagc tttagtgtat
     3721 cgtgtggcag tcagttctaa catacttgtg gagaaagatg ggattgaaaa tttagggtat
     3781 gagcctttaa tttactcaat tcttctgtct aaccttgttc tcaatcttag aaatatcccc
     3841 gaaaatggct aaaaatcagg gaaatattag gattttcagc aagacataaa taaaaatgtg
     3901 ctagaatatt atgtctatcc gataatttag tatagaaata tacttttttg agcgaatttt
     3961 agcattttat gaaccaagag catttaaata gaagagagtg gttggcaaaa aacaaccgat
     4021 tgagactcgg tgcaattttc attattttcg catcgcttct catcattatc tatgattttg
     4081 gttttactcg taagtctggt ttatcctcct cggtgaataa actagtaatc actggtcatc
     4141 aaaagctcag caaagaggaa atcgttgcga ttcttggagt gcaagcggga gttccttttg
     4201 aagattacag tttaaaaagc tttgaggaaa aactggagaa acatccgcgt attaaaaaag
     4261 caagcgtgac tcgtaggtcc aaagagcaac ttttagtatc tattgtggaa cgcggtgctc
     4321 ggtttattat aaattcaaac gacaatctct atgagattga tgatgattat aagttggttt
     4381 cagtagacga tatacgcgat acaaatctga ttgttttgag tggcgaattt aatatcggac
     4441 agaatcaaca ggtaagtaaa aagctaaaag aattctgcga atctgtagaa aatctatttg
     4501 agacctatcc acaacttaga gatagaatct cagaagttag attggatcaa gatggagaag
     4561 taacggttta tactcattat ccacagagga ttagagtgaa tatgggaaat aaattggaaa
     4621 gtattcaaat tagaaaatta tatgcaagtc tggctttttt tgaaaaccag gcagtgaacg
     4681 ttagattatt agacttaagg ggagatgacg cagtttatca ataaaatcta tgcaggaatt
     4741 agacaatagt atagccgcaa tcgatttagg ttcttctctt attaaggttg tagttggaag
     4801 ggctgtgagt gaatctgaaa ttgaaattat tggaacagga actgcttctt gttctggtat
     4861 taaaaatggc gcgatcataa acattgaaac tacaacaaaa tccattaatg aggcaattag
     4921 tcttgctgaa cttatggcag gacaagaaat ttctcaagtt attgtaaaca ttactggaaa
     4981 aacaattaaa gcagataatt caaaagcagt agttgctata acaaatagag agcgtattgt
     5041 tacagatgat gatattcgtc gtgtaattga agccgcgcaa ccaagagttc caagcgacca
     5101 aatggtttta catgttctct ctaaagaatt tgctgtagat gatcaaacca atattaaaga
     5161 accgttaggt atgactggag ttcgtctcga ggcagaagtt cacgtagtca cagcaggaat
     5221 caccactgtt cacaatctgg acaaatgtat agagggtgct ggactagaac aaatcgacaa
     5281 agtattgtct agctttgctt cctctgaagc agttctaaca agcggagaaa aagatttagg
     5341 gactgctgta attgatatag gggcaggtat tacggatatc gttatctatc aagatggtgg
     5401 agtatgttat tctggagtta ttccatttgg tggaaataat attacgagtg atatatcaat
     5461 cggtctcaaa acacctcttg aacaagcaga gatcatcaag aaaaaatttg gtcattgtat
     5521 gcatgaggct gttgaaccaa cagaaaaaat cgaagttcca gcggtaagcg gtagaccttc
     5581 tagacaagtt cttcgtcagg atttagtaag aattattgaa gcaagaacaa gagaaatttt
     5641 agagcacatc aatcatgaat tagatcgttc aggtaaaaaa gcatttttat ctggtggaat
     5701 tatcttaacc ggtggaacga gcttacttcc tggaattgat ttgcttgcag aagaagtatt
     5761 tggattgtct gtcatagtgg caagaccggc aggtcttgga ggattgtccg agagagttgc
     5821 atctcctgaa tattcaactg cagtaggcct tataaaatat gtagtaagaa gcattgattt
     5881 agatagcaag gcacccagcg aaaaatggag tagctcacca ggggaaaaag aaagccctct
     5941 taaacgagtt tggcgctgga tggaaactaa tttataaaaa taaaaatgga gcaaaatatg
     6001 ctgtatatgg atgaacaaag aacaagtccc gtagtaatta aagtagttgg agttggtggc
     6061 ggtggtatga atgccgttag tcgcatgacg aatgctaact tccgtggagt tgagtttatt
     6121 gtaatgaata cggatgaaca agttctgagc aagtccagcg ttgaaaatcg tgttcaactt
     6181 ggtaacaagg ttacgcgtgg aatgggtgca ggtggagatc cagaaattgg aaacaaagcg
     6241 gcacaagaag acaaagatag aatcgcaagt gttttaaaag gtgctgatat ggttttcgta
     6301 actgctggta tgggtggtgg aactggaaca ggagcagcac ccgtcattgc agaaattgca
     6361 aaagaactta aatgtctcgt tgtgggagtt gtaacacttc catttggatt tgaaggtcgt
     6421 cgtagaaatg acctagctaa aaacggactc aatatgctac gccaaaatgt agatacactc
     6481 attacaatta aaaatgattc tatcttcaaa gttgtagata agaaagcgcc tatcgactat
     6541 gcattcagag taattgacga tattctttta aatgcagtaa aaggaattag tgatattatc
     6601 aatcatccag gtattatcaa tgtagacttt gcagatgtta agactatcat gaaagataca
     6661 ggcgatgcga tcatgggagt gggagaaggt gttggagaga atagagttgc agaagcagtt
     6721 gagcatgcga tcaataacgt tctactcgaa gagacatcaa tcgctggtgc aagctctctt
     6781 ttaatcaatg tgacaggtgg aaacgatcta accattaacg attggaatga agtttctcag
     6841 atcattacaa gtcaagtgga tacaaatgca aatatcatca ttggcttaaa tgaggaagag
     6901 ggactcaatg aaaagattcg tgtaactgtg attgccactg gtttcagagc aaggcttcca
     6961 catacactaa cgtctctcaa aagagatgcg acaccggtta tcagtcagca acaaaataca
     7021 caaccacaaa aagaacaaaa ggaagcacaa cagcaaccat tagttcgccc aaaagttgtg
     7081 aatatgcctg atccgcatga atacattgag ccaactagat ctatcaaaac tcctgctcga
     7141 gtaaattctc cttataatta caatgaggat tatgatattc ctgcatactt aagaagagga
     7201 aatagagatt aattgatggg tgttgcgaaa atctcccctg acccctcttt aggaaagagg
     7261 gggatttccg ccgcgctttc agctactgtc aattatttgc attcgcaaat aattgacacc
     7321 gcctcaatcg ctaacacagg cattcctgtc taaaaatgaa atttcacagc attcgcattc
     7381 aaaactacaa gagcataaac gatattaccc tctacttgag cgatgtaaat ctactgatag
     7441 attcaaacca atttttcaaa aagtaatttt aacgatacta taaagttcaa cttacgatct
     7501 atgaatcaaa aacctatttt gagtagtata cttcttttat ttgcaatgct ttggctacca
     7561 ctcggacaag aagaattttt acttatgaac tggatgaaaa tagggaccta tgtgattcca
     7621 tttttattca ttgctacaac tgcatttaga cagagttcta ccaaaaatat ttttcaagat
     7681 acaaaactga taagtgttct tttactcatt gtatatctca ttcatcagta tgaagaacat
     7741 tggattgatt tatttggaaa tgagtattct ttttattatt cagtcaatca aatgctttca
     7801 gacatactca gtccttcttc tcccattagt cctctcacaa gagaagcaat ctttgtaatc
     7861 aatacatctc tcgtttggtt agtcggagtc aatgcgattt ggatgagtga aacaagaatt
     7921 tttcctacct ttgcgatgac agcaatcacg cttgtaaatg caataacgca tgtaattgca
     7981 ggaatagtaa aatggagtta taatcccggg ctagttacct ctattctttt atttattccc
     8041 tttactgctt tctattttca ttttaagtta aaggaaaatt ctaacttgaa aaagcaaatc
     8101 attacaagta ttctatgggc agtgatcgcc catattctaa tggtatctgg aatgttaatg
     8161 gcaaattatt ttcatgtaat caatcagaca gtttattttg tagttctaat tctatggtcg
     8221 atcttaccta tcgcaatttt tcgaactgta aacctagatt gatctctata caccgaactc
     8281 attttttaat ttatgaaatc ttatatcttc tggtttttct ttcaaacaaa tttctaaaag
     8341 aggaaaggcc ttatcctttt gacctaagct tttataagat tcaattaatg taaggagatt
     8401 tgatatattt tttgaatctc taagacgcag tctttctccg aagtcaatgg agtcttgata
     8461 tttacctaac gctctatacg ctagtgctgt atcatagagg tagtctgaga catgtggata
     8521 gtctgagatt aatttttttc ctaattctgc aattctttca aactgacttt ctgttttgta
     8581 tttttcaaag agtttcaaat cttcttttag tttttccacg ttcgatagtt tacctttgaa
     8641 atgtactttg agcaaagaaa gatcatccat taattttcca gcatttgtta tagattcaaa
     8701 aatcttttct aaatcaccat tcccattttt cacatggtct aaaaataatt cttcattttg
     8761 atttatgaga tcatattcct catctttccc aatgatcaaa tcatcccttc catcagatcc
     8821 aagtaagata atatcatctg gaagaagagg aaatatgtcg atggatatat gacccgtttg
     8881 cccttgggtt cctaatttcc tatattgatt tttaggttta ataaaatgag gaattccatc
     8941 tctatataaa acgatgtcag gatgttcaac attcataaaa tacatggtgc cagtctcttc
     9001 atcgattagt ccaaatgcga tagacataag catcgatcca tcaaaggctt caaatgtttt
     9061 atgcatttca ataaatgcat tttttaacca tcgctctgga tattgatttc ttccagatgc
     9121 agttgaaata gttctttgaa taatagattt aaatacagtc cctaaaacca aaacaccgcc
     9181 tgcaccttga atggattttc ccattgcatc tccatttaag aaaacaatgt attttttccc
     9241 ttgcagttca ataatctctg tgatgttaat atcaccacct aactcatatt cattttttct
     9301 gaatataaaa ctcttctttt gtttgataaa tgattcaatg tgaactactt tactaaatgc
     9361 tttgcattgg ctgagtggtt ctataagtaa agtgtttaag aaataatctc catcttgttg
     9421 ttcttttaag gcacttactt ctgtcagagc ttggtttaaa tctctggttc tttcttctac
     9481 tttctcttct agatttgcat agaggatgga attctctaca ctcactgcaa tttgagagga
     9541 tagaatttta agaatctcta ctctatctgg tgtaaatgca tttgtggtta gattgttttc
     9601 tagatagact gcaccgagca aattaccttg atttattatc ggatagcaca taacggattt
     9661 tggctttttc tcttttacat aagagtcatt tataaatatg cctgttttta ctgcatcatt
     9721 tagtaaaacg attcctttgg ttctagtaac gtagtttact atattggggg ataattcgtt
     9781 gtaaccttct aatgatatat gttttaaaac ttctattgta tcagtatttg cattgccctc
     9841 tgcttcgata taaaacttat ctttatcttt tagaataaag aatcctcttt ctgcacctgc
     9901 attctcaaaa agtattttca tcattttttc gaggagttta gctagttgaa tttctcctga
     9961 gattgtttga gatgctttta tgactgtatt taaatcgaga aaattaccct cagtcatatt
    10021 attattggtt gcacttgagc taatagtcat tgaatcatct atggaagatt cattattaaa
    10081 tttatgtttc gtctgacgtt taagttgagg gtattttgat tctaattgct taacttttgg
    10141 ttcacacccc cattttttat aagcatagtg agctgcattt agataaatat atgcaaaagg
    10201 ttccttattt ctttctaacc aaatagaggc tataaattca tttgccaatg cttcttctaa
    10261 aatatagtca tattgttgcg atagttcgat cgcttgatca aatgatgata atgctttatc
    10321 ctgattattt tcaatttgat aaattaatcc ttgaagtata tgtaatttat gaccataatt
    10381 cgcttggcaa ttttctcccc atttctttat tcgcttgtag tttttatcta aacgcttttt
    10441 ataatctttg ctttttttct tgtcctttgt gttcaatata agctttgcac agattagaga
    10501 atcaaaaaag acttgttcag ggacaaacat catgccaaac atagctgctt catgcgttga
    10561 ggctaatgaa gagtattcca aagcttgatg caaatcaccg aagaagtatt caagtctagt
    10621 tttgcaaaca tagaaatcaa agagagcgtt tgcatttttg gattctatta gttcaggtaa
    10681 tactttcgtt tcgtcataaa actctccaac tagcattaat ttattttcag ttagatctct
    10741 catgttggaa acaaattgct cattcaactg atataactga tatgcattgt gttgttgtaa
    10801 ggataaaata gattttcgat acttaataaa attttctgag agaatactca gattctctct
    10861 tatcataaca ccttggaaaa aaatgttatt gagtgagtag gaaccgtatt gtaaatcacc
    10921 tgcttccatt cctttttcaa aggcatgatt gtataacgct ttgttctctt tggcatgatg
    10981 cttccaatgg ttgatcatag ttgcaaataa gaaatatgtt ctacattcaa taggctttgt
    11041 ttgctcacca attttcttaa ttaaatctaa tgcaagtttg ccgaatttat aaccagcttc
    11101 aaaatctccc aaggcagacc cctgaatgat accgaaggct gcgtatgcga atggacttaa
    11161 aggtgtattt ccatgcgtta aagagaaatt taccatttta agaacgatta ctggaaataa
    11221 tgctggctgt gcaagaaagg ctggagcact taacgcattt aataagccca taatagccag
    11281 agatttcttt tccgttgtta atgtatgatt taataaatct tctgtctgtc tttttccaaa
    11341 ttttaattta aatttaatga tttctgggag gggagaaatt tcagttggat tatttggtaa
    11401 attcactcca aagcttttca aggcttgctt tagtataacg atagcctcta gcattttatt
    11461 ttgacttaca tacattgaag attttaattc atagattaca atcttatcca atggagtttt
    11521 agcttttgtt aagataagat cgaaagtttt ctcggcggat aaatattctt tagaaagata
    11581 ttcagatctt gcacgattag tatatagatc gagagtataa aaataattat cctcccacga
    11641 attttcaggc aattgattga ttgaattttg atagaaacct aacgctgcat cataggcatt
    11701 tgaggctatt gctttgtttc ctgcgattct atttaattct tttagcttta ctttttcttc
    11761 ttcacttgta atgtatgcaa gaccttggtt aagttgattg actattgtaa aaatatgatc
    11821 ttctaattta tatttatcca gcatagataa ataagtattt cctatcgtat aatgatatct
    11881 tgatctttct gtttcggaaa tgagtgtata agttgcttcc ctaattttat catgcacaaa
    11941 attaacgtca aattcaccta atctaagaaa tccttcattt gctagcagta gcaaatcttc
    12001 ttttgcctcc gattctgttt tatctaatat tttaaaaaac acttcttgct taaaccaact
    12061 gccaatacat gcagctaatt ttaatttttc gatagcagaa tcagaaagtt ccttaacttt
    12121 ttctaacatt aagtcaatta cgttttctga gattttaaca tcttggattt ttttgatatc
    12181 ccagatccaa ttgttatttt tgtattgaat aaattctttt tcgtaaaaac ttttaaataa
    12241 ttcatttacg aaaaatggat tcccttttgt tttttcaaag agaacctttg ccaattcttt
    12301 cgcaattata tcggcagagt ttaaggtatc actaactaga cttgagatat cagttagaga
    12361 aattggttct agtgatattt tattgaattt caatccacca ttttttaaat catctagcat
    12421 tattgaaaat ggatctttgg gtaaaacttc attatcccta aacgataaca ttaaaaataa
    12481 atatttcaaa tcaggattgg acaaaagagc ttgtattaaa tttatgcttg gtatatcagc
    12541 ccattgtaaa tcatctaaaa aaatcgcaat aggatgttct ttagagcaaa ttgcttttat
    12601 aaaattttga aaaacgatat taaatctatt ttgcgattct gttgtgccca actctggaac
    12661 tggaggttgt tctccaatta gtgtttctag ttcagggata acatctatga taatttttcc
    12721 attggttcct atcgcatcta gaatattttt tttccaactt tggatagaag tagaactttc
    12781 tgttaggatt tgttgaatta aactttggac tgcataggat atagccctat aaggaatagt
    12841 ctttttaaat gtatcatatt taccgcttgc gaagtagcct ttgtattttg ttataggttt
    12901 gttaatttcg ttgattaata ctgatttgcc gatgccggaa cgtcccgata tcagaagtaa
    12961 ctcagtgtta ccctctgcta cttttttaaa tgtatcaatg atttggttaa tttctttttc
    13021 tcttccgtat aacttttcag gaatttgaaa ttttggagaa aaatcaaatt ctccaattcc
    13081 aaaggactct gtatctagtg aagcaagtcc tttctcttca agtagcttga gacatttttc
    13141 taaatcgtaa agaattccac ttgaactctg atatcgatct tccggatttt tttgcaaaag
    13201 ctttacaatt atatcagaaa ttggtttcgg tgttttgttt ttttgaaaag gggaaatagg
    13261 atttttcgca atatgagaat gaactaattc gattggatcc tttgcaagga agggtaatcc
    13321 tcctgttaaa atcctataaa atgttacacc gagtgagtaa taatctgttc ggtagtcgac
    13381 agttctattc atacgtcctg tttgttcggg tgatatataa gctaacgttc cttcgataga
    13441 actatttaag gaaataaatg agttttgctt tactagcaaa ctagcatttc cgaaatctat
    13501 tatttttaat acttgggatg atttattgaa aataatatta tggggtttta tgtctttgtg
    13561 gactagattt aatttatgaa cttctccaag tgctttgatt gagagaatca taatgtttaa
    13621 aatttctttg attgaataag aagatacact attgtatagg gttgcaagtg attcgccacc
    13681 tatatcctca aagacaatgg caatactatt tttatatttc tcaatactta tcgttttgat
    13741 gatatagtct gaatttatat tttcgagtat atcatattca tgtttgaatt tacttaactc
    13801 gctagtgctt gggtagtcat tatttaaaat tttaattata acaggttggt cttttttctt
    13861 ccctctataa agaatactct tttcaccttc atgaatcttt tctgaaatag aatacccatt
    13921 taaatttatc atataaagtc cttaaataat tttattttga agtctttcca ccatcaattg
    13981 gaattgctgc acctgttata aaggaagcct cgtcactaca taaccatatt gaggtatttg
    14041 caacttcatt gacatttccc attcttccta acgcataaga ctttaatctt tcttttttta
    14101 gctgttctgg attatcagat ttcttataaa actcttgatc catttcagtt tctattccac
    14161 caaagcatgc agcgtttact cttattccat acttgccata ttctagtgcc gcacattttg
    14221 ttaatccgat tacaccatgc ttagaactag aataagcgga tactaattct ttgccgcgga
    14281 gacctagtgc ggaagataca tttaagacaa ctccaccacc ctgtttaatt atctctggaa
    14341 tttcatattt catcgataaa aaaacaccag taagattaat tgcaattact tgattccata
    14401 cgtcaagtgg atattcagtt accggaaata agtcacctga aataccagca ctatttacag
    14461 caatgtccag tttgccaaat ttatgaatcg tttgatttac tagatttttt atatcatctt
    14521 ctttagatac gtcagcagga acaaacaagc atttaccgcc attctgtatg atttcatttt
    14581 ctaatttttt tccttcgact tctcttctac cagatacaat aacagtggct ccattttctg
    14641 aaaatttaat tgcgattgct ctgcctattc ccgaggtgct accagtcact attacaactt
    14701 tatttttgaa tttcaattta agcctctata attattagaa aatttttaat tcaagttcta
    14761 tatacatcac tttcaatctt atttagtaaa aaggaattaa tttcatacaa attattgaca
    14821 atgtatttta ctttgatttt ttccatttca attctttgga aactaaactc tttatgcgaa
    14881 gcaactccaa tgaaaggtgt gttgtatcgc attgccgcct ctgctacatt tttttcttca
    14941 tcgataaata atacttcttg aaactgaaga ttataaatat cctttgtgat ttccttgatg
    15001 cctggtcgaa tatcttttgt ttgaatatat ttctcatcat caaaatactt ctgaatattt
    15061 gatgtatttt ttgtgaaata ctctttagag gcgcctccat aagaaacgac tttgtatcct
    15121 agcgacttaa ataagcttag aacttcttga atgccttcct taagttgaat agaattttca
    15181 ctttcatatt tttttctttc ggagtaatac aattggatta actcctcaat cgaatggctc
    15241 agatttaatt tttcttttat ataatcggca gcatgaattt gggttctcgc gaacacatta
    15301 ttttctattt ctgctgtata ttcgcctccc agtttttcta tcagattccg tgtaattgga
    15361 gctaatatgt tttctaaaac aacaccatcc agatgtaaaa aaatatactg aatggttttt
    15421 gttttagaca ttaaaaaatt tctctctctt caatcaatct gccgtatctg ctaattgctt
    15481 catttagatt ttcttgatcg attgatgtca tatcaaggat ttcaaatcca gaataatagt
    15541 tatcttcctg ttcagtgtca gacttcatcc aaatcatttt acaccttcca tgaacaaatc
    15601 cagctaaatc aaaatgcata aagaattcta aatgatcatt gtttgccatt tgcctcggag
    15661 ggtcagctcc ttctgaatga accataaaac ctttcttgga aaaattttcg atatgacaaa
    15721 cccatttttt agttttgtct ccatctagat agcgaacgga tatattgttc ttaaattttt
    15781 cgctcagccg aaatcttgga tctatttcat agacttcttc ttgtccagtt ttgagtcgct
    15841 tgtatacttt aagtggcaaa atagttgcca ctgtttttac tttactgatt tctacattct
    15901 cttggtagat agaaatctca atatcaacgt aactccagct tttatcttta ttcagcttta
    15961 agtcttttgc aattgccctc attttaattg gcatattgac tttagcatac tgaaagaatt
    16021 cactggttaa agattgtagt aagaatgtga catcttcgaa tggaacttta ccaaatttat
    16081 gacagcaggc aatagcgaat tgccgcgcag cttccgtaat ctgcatccct gagatatgtt
    16141 ttagatttgg tggagagaaa aatttgtggt caggaggagt ataaagattt gcaaagaata
    16201 cttctttctt gggcaactct tctactttct ctaaaatctc cgaaatcatt gccttctgga
    16261 cattattgat atgaaggctt ctcatattaa acatttttaa aattttaatt tcatctgctt
    16321 ctgttagatt attttggagt atataacgtt gttgcccttc atctaaggta taaaatttaa
    16381 acagatactc cttttcttct tctgttatag agccttcttc agtatattga cttgctcgtt
    16441 ctcgtgtaat tctctgagga attgttctaa gtagataagc ttcaattcca tctttgtctt
    16501 ttcttttctc ataataattt aatagaaatt gatcttcttc ctcagagatt tgggagtgta
    16561 ttattttctc aaataaatca acggaaattt ctctttgtaa tgctctgcga atatttgata
    16621 tgagtgagtc ttcttgaaaa taagttcttg tatattgctt atctaaaaca attacatttg
    16681 gtatatcaaa tttttctagt ttttcaaatt catgtatcat tgatactgtt cctagctaga
    16741 gattgtgtca gaagttgaat ctataactta ctcgaaacta atctttaata cttattctgc
    16801 aattattatt ttaaaatacc atgaataata aatttactaa gatagagggt aatcattgtt
    16861 atatgcttta aataagatta tgcactatct gacttgaact ttcaagctca atattctttt
    16921 aatttaattt tcacttcatt cgtattagga taatggtata ctatgagcta ctgaatattt
    16981 aggtgaggtt tgcttttaga ggaggctaag ggatgttatc cgtatatttc attacggtga
    17041 ataagaaaat gcgttatact ttccattcta ttagctaaat gttactacct gagaaatggg
    17101 tagcagcaat gatttaaact tcttgacatt ttagcgaatt gagtataagt ttcaatgaat
    17161 gagtcctgaa gaaaaagaaa tatttttgca aatgcagcaa aatattaatt cgattttaga
    17221 gttaattcaa accacacatg aaccaaacaa tggatataag attgttctat ttctggttcc
    17281 catttttggt attgtctttg ggtctactct actatgtctc attttcctgt tctggcatag
    17341 acagaggatg gagctgatta aatcaggctt atataagcct atagcctttg attttagagc
    17401 ctattccttt tttcttggac ttttgttgac atttacaggt cttactcttg caatagtatt
    17461 catcatagtt ctaggcaagt ctctggctat gttaggaggg ttaatcccat tagcaatcgg
    17521 tctcagtcta ttgacttatt acaaattcag tcgctaacag agcggcaaat actctgtaac
    17581 cccgcagatt gggattgcat ccaaaatgtt ctggctggtg atttctctgc cttcgaattt
    17641 ttaatggata agtatcaggg tatggtatat tcccaagcac ttaaattcac tggcaatcag
    17701 gaagaggcgg aagatttaac gcaagaaatt tttttgaaag cctttgaggc attatctaca
    17761 ttcaaaggag aatctcaatt ttccacatgg atttactcta tagcacgcaa tcaaatcttg
    17821 atgaagcata gaaaagaaag cacgaatccc aaaatatcca ttcaagatat ggaggaagtt
    17881 cacaataagt attttgataa aattaaaaaa tggaaagatc aattaaatcc agaaatgcaa
    17941 attctaaaac aggaaatgaa ttctaaaatt caatccatgc tttctagact tccgtctaac
    18001 tataagaagc ctctcatact ttattatttt gaaaacatgt cttacaatga aattgcagag
    18061 agtatgaata taaaagtaaa tacacttaaa agttatatct ttagaggtag ggaactttta
    18121 aaggaatgga tgcaaaaaga caatgaaaaa ggataaaaaa gaatatttta caaaaccaaa
    18181 tatttactct gtatttgaaa aatctcccat tgatatcaaa aagaattttt tttatagagt
    18241 tattccgtat acatatataa aatattctat ttatatgatg acctttttat ttttttcccc
    18301 gattttagtt gttttgttta actcagatct ccgaaaaaac gatgcaatct tttatcctat
    18361 cttactaaca ttatcctttt cttttttact tttttctctc ttatcaggtg caattctttt
    18421 tttctacaaa ataccttata tgcaagaatt gaaagataaa tttggctaca cagaaaggta
    18481 aattcaattc taatattaaa tttatttatt aaaatcacat gaaaaaaatc tatatattca
    18541 ttctcttcaa tctatttttt cttatatccc tatcaagtca ggaaatgact aaaagcaaag
    18601 tttccgcaga agacattttg aaaatgaacg agtcagaagc atttcgagtt attgaaaaac
    18661 taagaaaaga agaagcatca gaacttataa ctcaaataag aagcgttgtg cgtaaagatt
    18721 ataaagaaat tgataagttt tacttactga tttctcattt ggaaactatt aaggcaatcg
    18781 aagaagaaca agctagactc agaagtttaa atcttgtcta tggtcttggc ttattcttag
    18841 tgataggact tttactttat gttctagttt cacagagaaa gacaattcaa aatctaaatc
    18901 agcttttaaa agaatgatca agtattttag tatcgaagaa attcagggtc aagatcaagc
    18961 cttacaatat attaagcatt atgtaaaaaa tccacatgag attcctcctt tattaatctt
    19021 tcacggacct atcagtgtgg gtaaatgggc gttagctgaa agatttgccc gacatattct
    19081 atgtcttcat ggaagttcct gtgaaatttg tccttcttgt aaggcattta tgaataatca
    19141 tcatcctgat tatattgtat ttcctgctaa tcaaaagata gcaattggag aagataaaga
    19201 cccagctgaa tttactgtgc gttggttaca aaatcgtcgt ttaaattatg ttccacatct
    19261 ttctaaatat agaatcattt tatttccaga tgccagttta atcaataacg aagcagaatc
    19321 cgcactactt aaatcactcg aagagcctcc ttcacattct agatttattt ttatagtcga
    19381 tgatttgtta aagctcaagc aaacaatcat tagccgcgca gtttgtgtcc cctttcaata
    19441 tttaagtaaa gcaattttac aagatttgag cgcaaaccta cagctctata gggaggattt
    19501 ttttggcggc agtttaattc cttatgatat accttctgaa gcaattactc tgattcgaga
    19561 aaaagtctca cagaatttga atgattccct tcttttatta caattagaaa tgtggattcg
    19621 agagtataaa gaagatcatc ccgaatggcc agaggatttt gactatatag aatttttaga
    19681 attaatttct acaatcatga tctatgaatt tagcaaagca aactttgagg aaaatgtaga
    19741 taaaatagaa atcctctatg agtttaaaat ggaattgcat aagaatatac cagggttaga
    19801 aaatttttta ctcagtgatt tgtttcacaa acttacccgt gactgatgta taaatggttc
    19861 aagataattt ttataatctc acaccagatc tcattttaga tttactagag aagaatgggt
    19921 tttcacctac tggtcattgt tttgctttaa atagtttgga aaatagagta tataatgttc
    19981 gtttggaaaa taacgaagtt gcgattgtaa aattctatag accaggacga tggacgaaag
    20041 agcaaatctt agaggaacat tcttttttat ttgaactaga taaaaatgaa atccctgttt
    20101 gtgctccgaa agttttttca ggaaaaagtt tattcgaaat aaatggtatc tattgtgcaa
    20161 tttgggatag aacgggaggt agagcccccg aagaactgag ctatgaagaa tatcaaatgc
    20221 taggacgtta tttaggcagg cttcattctg ttggtgctaa tcaaaagaca aaatatagaa
    20281 atcattttac ctctcagttg tatggaatcg actcatttgc cttcttaaaa aaaaatggtt
    20341 ttattcccag ccatttagta aatcgatatg aagagttagt caataaaatc tgtattttat
    20401 ataatgaatt gattcaagat gaaccctcta ttcgaattca tggagactgt cacaaaggaa
    20461 atattctaaa aggagaaaat ggttttttct ttttagattt tgatgatttt gtctcaggac
    20521 cggcagttca agatttttgg atgatgcttc ctactgataa aagagaaaaa gatattgcta
    20581 gagaggcttt aataacaggc taccgagagt ttagagattt taatgaagaa tctttgcgac
    20641 taatcgaagt attgcgagct cttagatatg ttcattatag cgcatggatt gcaatacgtt
    20701 gggaagatcc ttcttttcca aatgcatttc ctcacttcgg aacaaatgag tattgggaaa
    20761 aagaaataaa agatttggaa attcaattgg ttttaatcaa taggggagaa tccgaggatg
    20821 tagagatagt cttagcggat aacgaagata tacctctaac caataaagat tttttttggg
    20881 atttagaatg atttactatg aagaaactat ttctactttt attatttaat tttattcatt
    20941 gcacaacagt tggatttcac gacgatagcc tacgtaaatc tattgacttt ggagaagagc
    21001 aacctctcta tgtttgtgtt cttcacgaag atacagttag aaaagaagaa gttttggagt
    21061 tggaaaaaga ttgggcaaaa gaacttgctc tgtataaaat taatttgaag tttaaacgga
    21121 ttcaacaggt agaacgattt gctttttata caatcgatgt tttagaggga cttagatatt
    21181 acaaagtagg tcctgaatgt gagagaattg tttatatggt tggtagaacc tggggtgata
    21241 ttgcgtatga agttctaaca ttaggaattt tctatggcat tggtgttaag cttgaagtcc
    21301 atggggttgt tgaaacctat acaaatacta gaggtgtaat aaaagcaaaa tatgttgctc
    21361 tcttacagtt tttatttact agtcctagtt caactatcgt tcatgaggga tatcacctta
    21421 tgggatgtcc acatatgtta tggaaagatg attgctataa aatcattcaa gaagcaaaac
    21481 aaaataattt aaagaacaca agaccagata agatatttag tgttcaatca acgggtaata
    21541 aatctaactt ttattcaagt gaacaagtaa attatttttg gtctagtaat aaagaaatac
    21601 ctaagtaatt aaaactattt cttatttgga ggaatgtttg gtttaacgct agtctctgtt
    21661 acaacttttt caatacttcc atttgaatac ttatatataa ttttctcttt ttctttttga
    21721 atctggatat ttccgtttgg aagaatttgt agagtatcac aataaacata atttggaaat
    21781 aattgttcaa acattccttt ttgatatgta taaatgtgac aaagaggagg ttcgcctatc
    21841 gtttcaaatt gagcccaaat ttctggaatt tcatctttat caatatctat tacttttaaa
    21901 ttcttcttat caccggctaa tagagaatgg attgtctctc catctctcca cactagataa
    21961 taggtaactg aataattacc aaatcctttt ttagttttag ttccggttgg atagctaatt
    22021 atataaaatt tcttcttttt accattgtct ttaaaatgaa tagtctctgt tgtcacctta
    22081 ccataattaa ccgcttgttt ctcgtctggt tgacctttta ggatttcatc taccatttct
    22141 ttttcaaatg atttaagttt ctttttgcga ttcttaagtg tataggcaga aaaagcttct
    22201 ggtgaaataa aactacctcc ctttgcccca atccaaccaa ctacaatgtc ctccgaggca
    22261 ggctttagtg attttaattc aaaatagtaa tattcacttt cgaagtagat tttttttcga
    22321 tagacaggca caacttctcc tttctctgga tagtcaggca ttgctcctaa aatatcccta
    22381 ccaggttttt tataaaaaat tggttctgct gctttaatta gaaaataacc aattagagtt
    22441 tgtggtagaa aatcttctct atcttctttt acgatagttg cccctgaaaa aatccaacct
    22501 gtttttccat tgtattctac ttttagccaa ttcccgattc tattttcaat cgattctctt
    22561 tcttttgttt cttctagtat ttcacctatc acttcttcag gaatcactaa aagttttttc
    22621 ccatttaact ttggtttgtc tctaagaggg gttccatttt ttgcaattac tttaaagaat
    22681 gatttttcct ttggctgtgg ttctgatgaa tctggtttgt catcatcatc tgcatcccgc
    22741 atatcgtctt ctttttttga atcaatattt ttaatagttt cggtgattat ttttttatct
    22801 ttatccgctt tggttttctt cttatttgcc tgcgcactta tgggacttat ttgcgtcagt
    22861 atgaaagcaa gtaaaagaat aattaggttt tgtgggttca tggtgtttct cttaaatcta
    22921 gatttcagga aataaggttc tgattttttt catttgtgtt tcattacgtt tagttggatt
    22981 tgtaaagatg gaaaatttag atgttcctac taaagacaaa tcatctccat ttcctaggat
    23041 tggaattagt tcagcaatta atcgcttcgc agttgcatta ttatgcgtta gatgaccaat
    23101 aaccatctca actgtcactg gttcttcatg ttctttccat gaatcataat ctgtagacat
    23161 acaaaccatt tgataagcaa tctcagcttc tctggcaagt ttagcttctg ggagaacaga
    23221 catgttgatt acatctccac ctatcattct atacattttt gattctgctc gcgtagaaaa
    23281 taatggacct tccatacaga ttaacgtttt gtttttatgc gtttttattt ggagtttttc
    23341 gcataaggaa taaattcttt ctgatagatt tttggaaaag ggatcaccaa aactagcatg
    23401 agccacaact ccatttccaa agaatgtaga ctctctgatt ccctttgttc ggtcaatgat
    23461 ttgatccggc aatacaaagt ctctaggagc gatttcttcg cgtaagcttc caacagaact
    23521 aaacgcaaca atctcctcaa cacctatcat tttaagcgca gctatgtttg ctaagtttgg
    23581 aatttcggat ggggaaataa aatgtcctcg tccatgtctt ggaagaaatg cgattaattt
    23641 ctctctgtat cttccaattg tgattgtatc ggatggtttt ccccatgctg tatctggaaa
    23701 atagttttca ataacttcca ttccttccaa ttcatataaa ccagttccgc caattatagc
    23761 cgctcttact ttgtctttca catctcactc caatctattg tcttttttat gaatttctaa
    23821 aatataacta tagtccagtc ttttttgatt tagcttgact catacaaaga gattttaaaa
    23881 tgaatgcatg aaaatcatcc tctttttttt actttgggtt ctatctacaa tttctgtttt
    23941 ctcggaagat aggaaatttc tgtttagaga tcagatcgtt caatcctctg atgctttttc
    24001 tatgctaggg gatagccgaa cacaatatgt ttttgaactt ggtgccgaag tagattggga
    24061 ggataagcaa tacctaggaa agtataaaac ttcttgtagt gaaagtaaag ccggaatcca
    24121 gaatgcggga gtcgctggat ctactactga gcattggtta gagtttctaa aaagtgaaaa
    24181 ttataaacct gaagattttc atcagaatat agttttaatg attggtggaa atgatatact
    24241 cagatactcc tataaatggc gaggatttac atttaataag acagatgctc ttaataatgc
    24301 gttagatgaa atacattcaa gagtagttga gatcgtaaat atattacgac aatccaataa
    24361 aaaagtgatt atccaaacac actttcgagt aaatcctgct ataaaagagg gaaatagtaa
    24421 atctttaaat gaaggaattg atggacttaa tcttagattg ctagaaacct atgggaaaga
    24481 tttagatggc gatgttactg ttgcttatat acctcctgaa tttccagctg cgttattttt
    24541 agatttggtt catttgaatc cgtttggata tcaattgcac tcttattttc ttgctcgtga
    24601 attggagaga agatgttggt ggtgattctt tggggaatca ttagaagctc tatgatttta
    24661 ttttacttta aactttatta tgaaatgtta ttgattcaaa atctgatata aattcctaat
    24721 agtaaaatga ttcaattcga agttatctcg cgatttatgc ttttctttcg cataacatta
    24781 gtttgccttt tctttttaca ggcttgtcat tcaaccactg aaaaagagca tccaatcgaa
    24841 gatacacata ttttctactc aaattataaa aattctaaag gtgtggtgat acacaaaaat
    24901 ataaaaattc aaacaaggct aatagatgtt acccaagaat tagagtcttt agaaaaaatt
    24961 gagattcaag aatggatttc aaaaattgac aaagctcttt caactgttaa tcagaatatt
    25021 gagcatttaa atgtagatgg atggaaatat attaccccta aaggaatttt aattttagct
    25081 tttacagcag cagtcgtaat tcctattccg ggaacatttg agactttgac aacaattgga
    25141 tttattacaa tgggagtgca aagaatcaga gaaaaagagg aagataagca aaatagaatt
    25201 cgtctatcgg atcttttatc agagtgttct gaggcaaacg ataaattgac tcatttaaga
    25261 aaaacaactc ttccagatta ccttaaaaaa tggagtaaaa ttttttataa taaagataat
    25321 catgttagaa atgcaaatga tccagccgat attcgcgtgt atgatgttct ttggaataga
    25381 tataaaatag aaaaagaaaa tttaaaaaat acaattgatt tagaactaga acatgcgaat
    25441 aatcagtgtt attttgtcga aagatcaaaa gatttttaag caggttcgaa aaatgctaaa
    25501 ttttcaggtc cttcatggat gactatcttt aaaacttttc cctttgcttt cttaacttct
    25561 tctttgattc cagaataaaa ccaacgtgca atgttttcag atgtaggatt aattcctttg
    25621 aagtcggaat gatcgttgat taatatatga tcaatggact gaatgagttg tgctaatttt
    25681 tgttttgcag ttaaaaaatc gaaactgatt ccgtcggggc gtatattttc ttttcctgct
    25741 agataaattt ctactttcca agaatgacca tgtatggctt catcactacc gtctgcatag
    25801 tatttgtata aataatgaga ggattcaaat cttccttcaa ttcgaatata aaatttgcct
    25861 ttttcttttt gaaacatatt gttaatggat aatagaattt gatttttgtc aaatacattg
    25921 aatctaaatt aaatttaaac atggatgata ggaatgtgtt tttactataa cagaagccat
    25981 ttcgattttc cttttttaat gcgtgaaaaa atctctaaaa tagaaataat ttggttttgc
    26041 atatcgccta ccgacccttc ctggaaaaac gaatctataa aaattttcta ttcttgactc
    26101 caaataaata gctcctttat ttaaatcctt gtttgagcca tttgtgctgt gaaggtataa
    26161 ttttataaaa atctaaatga aacactgtat ggcaatgaaa tcgtcgactc ttagatttta
    26221 attgtatcaa taagattgta gatatttcct ctttcaactt ttcgatcgat taatgcgatg
    26281 agacattaca caaagaagtt ctattttatg tgaattgggt gaaattcatc agaggatagt
    26341 cttatcccaa tttttcgttc cgcggatcaa aaaacagagt cgattctacc taggatgatt
    26401 gcatctaaat tcgtttttaa tttttagcca attgaaattt tctaccacaa gtgaagtaat
    26461 tcttttgagc ttttattaca ggataaaaaa agaataggct gaatagtttt gaggcatatc
    26521 gatcaggaaa ggattgtttt tatagagtta ttaagctatt tacactagaa tacttaaatt
    26581 tagatcttgg gttctgtaag aatcaatttg taaaactcaa ttatttcttt gttgctattt
    26641 ttagaattgg tctaaagtat agaaatactt tcaaacattg gaggtttggt gtgattcaat
    26701 ctgcacaaaa gttttataaa gaaatgactg ttggggaagc tatgcaagtg caccctgagg
    26761 ctggacttgt attttctagc taccatttag gtggttgctc tcattgttca atcaatgaga
    26821 ttgaaacaat tgaacaagtc tgtcaaggct atggcgtaga agtagatgtt ctattagata
    26881 gtttgaacaa tctatttgcc gaagacgaag cttaataaac ttaaattcgc gggtttgaga
    26941 ttctcattcc cgcatttctt ccttcctttt tcatgctaga aaaaaatgac attcagctgg
    27001 gtatttcccc aaagcctatc aacgagacag aagagagaca actaagtctt ctattgaatc
    27061 taatccgaaa tagacaagga ctttcctttt ttcaaatcag acagattatg aaggaatatt
    27121 attccaatcc taatttagag tcggatcaaa aaaaactgca tagagatatt gacgaattac
    27181 gcgaaaatgg ttttgctata aagtttttta aaataaacca tgattcacaa gaaatcaatg
    27241 tttatcgaat ggatgattcc caaagagatg ccagaataca gatgaccgaa gaggaactcc
    27301 gtaagttttc ttttttaatc ttaaaaaaat cagaagatgg agcgtctaac gatttatttt
    27361 cagctgcgca aaaaattttc tcaagaagct tacagcattt tccttacgca aataaaaaga
    27421 taaaattaga tgaaccttta gagaaagaag aagaccaagc ggtttcggat gcattgtata
    27481 aaattatgag tgcaataaaa aataaaactc ctttgcgtat tcaatatttt aagacgaatc
    27541 catctgaaag ttattatagg gacatagatc cactacagat aataaaaaga aattcagaag
    27601 atttttattt gatcgtgtat gatcgagaaa aaaaacaaaa gcgccgtttt ttgattccaa
    27661 agattcttaa agttatagaa caaagcggag aatttttatt ttctggaaaa gtcactgaag
    27721 atgatttaaa ttatcatgca cttaatttca acatacatga aaaagaggaa ctaatagtta
    27781 cttgtaatga gagtgactct tggaaattag aaaaatttct atttccacat ccattcagaa
    27841 aagttgacaa tcggttttat ttttcaacaa caaatcgagc tgcactgttt aattttattt
    27901 taagggaaaa tgattgtatc caagaagtaa actcagaaat tttcgtgaat gaattgaagc
    27961 aatatctatc tgaattgaaa aatcaatatc taaaagctga ttcaattcaa atatagaaag
    28021 taaaatagaa aaatgtttct ttttgcattt ttatgcgtta ggcgattatt aaattaatta
    28081 gtagaactca aaatgattac aacaaaggaa ttaatacaag atagggagat taaaggtcta
    28141 agatttatta gctattttcg actttttttc gttttttcga atgtaggctt tacccttgta
    28201 gttggaaaaa gcgtaatgga aagagtcgtt gtaggaatta tttctctatt atctatttta
    28261 tttttagtat actttcattt tttactgaaa gaaaaaagaa aacttggatt tgtaggagcg
    28321 acaagcgctc tattcgatac aattatatta atcattactc cctatatttg gcatcaatct
    28381 gttggaggtg atctcgtatc tagagcctat attctaaaaa gtccttcttt tattggaatg
    28441 tgttatgtta ttttaattat acatggattt tttgcaatca gacctttaca ccctcttatt
    28501 atttcttttg gtgtaagctt gggatgggtt agctttttag tctttgcatt gcaagatcca
    28561 agagtagtta tatcctccga ctttgttagt tatatgttag gagatacaat cagtatggaa
    28621 tttttcataa catttctctt tagcttttcg ttgtctggtt tatttttgag tttctataca
    28681 tatcaatcta gaaaattaat ttttgaagcg gtcaatttag agaaagcaaa aatgcaagta
    28741 agtcgatact tttcacctaa cgtttttaat aaaataacaa ctacggaaga aaacctacta
    28801 accgctttag ccagaaacca aaaggtagca gttatgttta cggacattcg agattttact
    28861 agtattagcg aaagtctttc tccagaagaa gttgtccaac tcttaaaaga gtatcatgct
    28921 agaatggtga atattatttt tcagtttggg ggaacattag ataaattcat tggtgatgga
    28981 attatggcta catttggaac tccagaagcg caaccagatg atgcctatag agcagtgcta
    29041 gctggaattg agatgaaacg ggcattagcg gagttaaact tacagaggtc taaaaagaat
    29101 ttaccaatca tcagacaggg aatagggatt cattttggaa atgtaatcgc cggcaatatt
    29161 ggaacggaaa atcgaatgga atatactgtg attggagata cagtcaatct agcaagtcgt
    29221 atcgaaagta aatgtaaaga gctttctaca gattttttga tttcggatgc agtgaattca
    29281 gaattgcagg gtagaattcc gacaaacttt aagggagagc atactgtgaa aggcaaatcc
    29341 atttctgtga gtttatacga agttcaagtt gaggtttaat tagccgaaga attcattcag
    29401 ctatattttt atcatttcct ctgagggctc attttttaac taaatagaac tgttcacaga
    29461 ccaatctgcc attaaaaagt aaatgactat tcaccctttt tttaaattct ggtaggaagg
    29521 gaggacaaat atgtctgaag aaaacaaaga aaagaaaaat aagaaaattt cgaaaatgag
    29581 ttctgctgaa ttggaagcag ctcttaaaaa agcgaaagac aataacaatt ctgatatttc
    29641 aaaatacgtt caacacatca accaaagaaa agctgagcta aaaaaataaa tttgcaggag
    29701 ttgctccact cctttaggcg gaatgattga ttcaatttat taatattcgt catcaatatg
    29761 gagctaacgt tttatacgat ggcttctcat ggcatataaa gccaaatact aagataggtt
    29821 tagtaggacc aaatggagta ggcaagacaa ctctttttca aatggctgtt ggtttactca
    29881 aaccagatga aggagaaatt gttcgtgcca aagacacagt aatttcttta tttcaccaaa
    29941 tccctgattt taatggcgaa aagtctgtaa ttgaaactgc cctaacatcg aatactcttt
    30001 ataaaaaata tctagaacga aaagaagaaa ttgataagcg ttttgagaca acagatcatg
    30061 agagtgatgc atttgaaaaa ttactttctg accaagcaga attagaagag tatgcccata
    30121 cgcatgactt acataattta gaagttaagg cgaagaaaat tctaagcggt ctaggtttca
    30181 aagatgatgc atatttaaga caagtaaaga atttttcccc tgggtatcat catcgtattg
    30241 gtcttgcaat tgcactttta aatccttaca atttactttt actcgatgaa ccaacaaatc
    30301 atttggatga tgctgcaaaa gaatggctta gagacttttt gcaaacgatt aaaactacat
    30361 ttgttattgt tacccatgat cctgagtttt tgaatgaagt aactgatacg atagctgaaa
    30421 tatctctgaa gggcgtgata gagtttaaag gaacgttaga agctttctta gaagagaaaa
    30481 atgaacttca tgaaaaatta aaaaatcaat atcgcaaaga agaagcctat ttaaaaaaga
    30541 gaatggagtg gattgatcga tttcgtgcac aggcaacaaa agcaagacag gtgcaaagtg
    30601 ctatcaagaa attagaaaaa agagatgcag tagaaaatcc agaagagatc ttttggaata
    30661 agaaacctgc ctatcgattt aatcatattc cagatggtaa aattactttt cgtttagaaa
    30721 atgcaagttt ccaatatgat aaggatggac gtgttatttt taaaaatgcg gatttagaag
    30781 tatctgctgg tgaaaaaatt gcgttagttg gacccaatgg tgcaggaaag tctactttaa
    30841 tgagagcgat attaggtaga cataccttat cttctggctc aatttatttt ggacccaaaa
    30901 ctagaattgc ttatttttct cagactcacg gggaagattt gtatccagaa ttaaatatga
    30961 ttcagtctat tttgaaagta tatccagata tgtcagacga ggcagttcga actatacttg
    31021 gacatttttc tttttctggt gatactgttt acaaacaagt ttccgctatg tctggaggag
    31081 agcagagtag acttaggctt gctcttatgg tattgactcc tgcgaattgt ttatttatgg
    31141 atgaaccgac aaatcatctt gatatggtaa ctcgcaatgc tttaaagcat gcattgatgg
    31201 aatttccagg ctccttgttt ataataagcc atgatcctga ttttctaaaa ggtttgtgca
    31261 atcgaacctt tgaactgtcg gctggagttt tgaaaaatct aaactgctct tttgaggatt
    31321 atttaaaatt tcataaagag ggtgtttatg gtgaggaaga tcaatctgta aaacctgtta
    31381 aaacaaaaga gacaaatgca agtcgtaata atgataaaaa tagaattaaa aaaattcaaa
    31441 aagaaataaa tgatattgaa tctagacttg aacttttgga gagaaacaaa aaacatctag
    31501 aagaagtgtt atctgatcct gccttcttta aaaatcgtag ctaccaaaac gaattagata
    31561 attataatga tactaaaaaa gagattgcat ttttgacgga acgatgggaa atcttaagtg
    31621 aggaaataaa ttaatgaata tcagtcctac tgaattaaag aaaaagttag acgaaagaga
    31681 aaaagggaat gatgatttta ttttggttga tgtaagaaac cccaacgaac aagagatcgc
    31741 tgttattcca ggaactgata tgctgatacc acttccagac ttcgatgtag aaatttctaa
    31801 acttgagaaa tataagtcag aaggaaaaga aattatagtt tattgtagaa gtggcggaag
    31861 atctacaact gcctatggca aactaaaaag aaatggattt accaacgttt ccaatctaga
    31921 gggaggtatc ctatcgtatt cagatcaagc cgatccttcc atcccaaaat actaatggta
    31981 taattttctg atttactatt ctataaacta acggatgatg acgattctat gcagctagtt
    32041 ttgcttatag aaacagatac tcaatcattg agagattatg ctaagtatct ttccggaaat
    32101 cgattccgat tagtggacac agataatgca aaaaaaggat tagaatatgc taaatcagcc
    32161 ccacctgatt tattgcttct tggtttaaat ccacaggaca aagactcaat tgaaacactc
    32221 atttatctaa aaaaagatcc aattacaaag catattccag ttctagcttt aattagtttt
    32281 ttcaatgaat cctttttgga gcaattaaag aaaataggaa tcagtgatca tcttgtgaaa
    32341 ccatttaagc aacatacact tgttccaaaa gtaaatgaac ttttaaacct atcggaaaag
    32401 attcgagcta atattgtaaa gcacacaacg catcatattt ccataacact aaacaccgat
    32461 gacagagttg tcatagtatt tcgttcagga attaaaaatt ttgtgttacc agagattaga
    32521 aatgtcttaa accatgattt tataaaatca tccattaata aacacattgc aatagacatt
    32581 cacacagttc atgaaatttc tacggaagaa ttgcagattc tagagaaaat cacaaagctt
    32641 ttcggtgaaa agaaaatttc tcttatcaca ggaagtcatc ttggaaatat tatgaaaaac
    32701 agtgaacttc ccgaatttgt aaatttattt ctctcaatgc atgattatga gttattctta
    32761 gagaatccgg atttggatga gtagcacttc ctaatttaac gtcactttga tgtgacagaa
    32821 aatttaaaag attaccaccg atgaagacga tgagcaccga taaaagatat tataattcaa
    32881 aatcaatcat ttccgtatcg ttcagttatc ggtgtcttcg gtggtaattc ttacatcgag
    32941 tttatattaa cctaaataac gccagttcgg cgtaacaaaa aatttaatag attaccaccg
    33001 atgaagacga tgggcaccga taaaagatac gaattcaaaa tcaatcattt ccgtatcgtt
    33061 cagttatcgg tgtcttcggt ggtaattctt acatcgagtt tatattaacc taaataacgc
    33121 cagttcggcg taacaaaaaa tttaatagat taccaccgat gaagacgatg ggcaccgata
    33181 aaagatacga attcaaaatc aatcatttcc gtatcgttca gttatcggtg tcttcggtgg
    33241 taattcttac atcgatttca cattaaccta aatacgcttt aatactatct cgaatcgttg
    33301 taaataccca ttcgaatttt gctggatctt cttctaacag tgtgattctg aatccatctt
    33361 ttttacaaca gaaagaagaa atgggaacta cgcaaatacc tgttgcggct aacaggttta
    33421 acacaaaacg tctatcgttt gcaatgtttc tgagaagagg ttgaataaat tcttctgctg
    33481 atttatttgt gatagttaat ttcattttat catttagaat tccatcttcg aaaagaacgg
    33541 ttaagaagaa accacctttg ggctccacaa ctttaattcc ttttgttcca gccaaaatac
    33601 taactgcttt ttttgccctt tctttgtatt tctgatttct ttcatttaaa tgtggaatga
    33661 aattaggaga agcgtagatt tctggaattg ccatctgagg aagggtagtg gaacaaacct
    33721 ctaacatctt tgcatctaga agtgtcttga tatatctttc gaataacgga tcattttttc
    33781 tgttaaatac ttcaatccag ccacatctag aaccaggcca tggtaattct tttgagatgg
    33841 agcgtagtgc cataccacaa actttgtttc caattacttc tgataaatga atggaaccag
    33901 acccagaata gttcacatgt gcataagtct catcacaaat taaaatgcaa tcatacttct
    33961 cacatatgtt tacaatttct ctcataatct ctttgttgta aactgcaccg gttgggttat
    34021 ccggattaat tagaagtata cctgctatag agtcattgta ttttacttta ttttctatat
    34081 cagaaatatc tggcatccag ttgttatttg gattcaattc atacgttagg tgctcataac
    34141 cactgtgtgc agcctctgct gaagaataag tagaataagc tggacttggt cctaaaattc
    34201 tagcttctct tctcataaat ccaaatatct ttgcaacagc atccccaaga ccattgaaaa
    34261 ataaaatgtc atcaggagtg atttgcactc cacctctttc atttactttt tgagcgagga
    34321 acttacgcgt gttttccgct ccttgtgtag ctgtgtaagc ccaacttata tctttttgaa
    34381 ctagattgga aacaatctct ttgatccatg gcgcaacggc ttctcctttt tgaattggat
    34441 cgcctatgtt ttcccatgta atgtttacgc ctaacttctc tagttgtttc ccaactccta
    34501 caatttgacg aatttcataa attaattcat ccgctcccgg atgcacaata tttcttctca
    34561 tataaatata attccttttg ggtgcataat aaagatttcc accgatgagc accgattaca
    34621 ccgatgggtt tttatggatc aaaaaattat gtatacactt ttaaataaaa attctatctg
    34681 tttgcttttg ctcttgaatg atttagaacg atagccgcat ctacttttac tgtagtgaga
    34741 tatagaatcc gttcgtttca atttatttga atccttttat taatgttgaa aatctaagcc
    34801 tgttttctat ttcgaaaata ttcgatcacg gcaggcatga ttgatatgat gataattcca
    34861 aatataacat aagtgaaatt ctttcgaact tctggatggt ttccaacgaa ataaccagca
    34921 actaaaaata tactgatcca aagaagacca cctacgatat tgtataaaat aaatttagaa
    34981 tagttcatcg ttccaatgcc cgctacaaaa ggagcaaagg tgcggacaat tggaataaat
    35041 cttccaatga taattgtttt gccaccatac ttatcataaa agtcctgcgt cttttttaaa
    35101 tgatccttat tgataaaatg aattgtctct agtttaggtt ctagaaattt tccaatccag
    35161 tagtttactg tgtcacctaa aatggctgca attgctaaaa taaaaaataa taagaataca
    35221 tctaatgttc cacgcgcggc aatggcacca atcgcgaaaa gcaaagaatc tccaggtaaa
    35281 aaaggagtta ctacaagtcc agtctctgcg aagataatga taaacaatat gacatacgtc
    35341 agagttccat attcggttgt gattgtatcg agatgtctat ctatgtgcaa gacaaagtcg
    35401 atcatgttct ttaaaagttc cattgtatcc tcttcatttc acataaattc aaatcaatag
    35461 attctgaaaa tagaaaattt ttccttgtca tataatagtt ttattgcctt cctgtatcca
    35521 taaggacaag aaactaaatg tcaaaaaaag taatcgttgt tggtgcatct agtggaattg
    35581 gagctgagat cgcaaaggaa cttctaaaat ctggagcata tgttacactg attgcaagaa
    35641 gagaaaaaga actcaatcaa atcacctctg aaattccaaa ttcaaagtat tgtattatta
    35701 agcatgatgt tattaatttt tccgaagctc ctaaactttt tcaggattca ctgaaagcga
    35761 tggaaggttt ggatgagatt tattactgtg caggtatttt agaaaaagtc gcagtagatg
    35821 aattttcaac agagaaagat attcgaatgc taaatatcaa tactcttggc gcatttgctt
    35881 ggctcaacct tgcagccgat tattttcaaa aaaagaattc tggtaagatt attggaatat
    35941 cttctgttgc gggagatcgt ggtagaattg gtagtccagc gtataacaca agtaaagccg
    36001 cactcaatac ttatttagaa agtttaagga atcgacttgc tcgtaaaggc atacaggtgt
    36061 taaccgtaaa accaggattc atagacactg atatgacaaa aggaatgaag ggtctctttt
    36121 ggcttatttc ggcaaatgaa gcagctcgta ttattattaa cgctgcggct aacggtaaag
    36181 aagatattta tgtgccagca agatgggcat tggtcggatt gattatccga atgataccat
    36241 cttttatatt taaaaagctg aatatctaag tttcaaattt ttataaatat atagaattaa
    36301 aaaaatcgcc tatgatcaat tttcgcagat ttaaaaattt catcactgcg cctctgagac
    36361 tctgtggcaa aggataacgg ttattaccat gaatgaaaac acagcaatct attttccaat
    36421 ctcaaatcca gtaaaagtag aaacttgggg aatgaatcat ttctcaatga gtagagtcta
    36481 tcgtccaaaa acagaacggg agattagaga tttatttgcc tatgctcaag caaatagaac
    36541 aaaggttgtt tttcgcggag gtggatgtag ttacggagat gcatcggtga ataacgcagg
    36601 tcttgttgcc gatatgcgag attttaataa gatcattagc ttcgatgagg ctactggaat
    36661 attgactgct cagtctggtg taacaatcaa acaactctgg gagtattcga ttgaaagagg
    36721 tttttggccg ccggtcgtta gtggaactat gatgccaaca ttaggcggag cacttgccat
    36781 gaatatacat ggcaaaaata attttgcagt tggacctatt ggggagcata tcgttgaatt
    36841 tacatttctt actccaacag gagaactgat tcgttgcaat aaagaatcga ataaagattt
    36901 attttatatt tttatcagca gtcttggaat gcttggatgc ttcatcgaaa tcaaaatcaa
    36961 gatgaaaaaa atctattccg gcaaaatgaa agtaaccccg attccagtga agaatctaaa
    37021 agaaatgatt gatgtttttg taaaaaatat tccagattcg gattatctag ttggatggat
    37081 tgacgcattc ggatcaggag acggattggg aagaggactc attcatagag cagacaacct
    37141 aaagcaaggg gaagataaag actttccaga aaattgtaaa ttagataaac aaaatcttcc
    37201 ttctactttc ttcggagtga ttcctaagtc ttggatgtgg atttttatgt atccattttc
    37261 gaatgcactt ggaatgcgac ttgtaaattt tgctaagtat ttacttggat ttgtttctaa
    37321 aaagccatat atgcagggac atgctgagta tgcattctta ctcgactatg ttccgaattg
    37381 gaaattcatt tataaaccag gggcgatgat tcagtatcaa atttttattc cagaagagaa
    37441 tgcgttatct ggaatgactg aagtattatc tcaatgtcaa aagcgtggac ttgtgtccta
    37501 tcttgctgtt tttaaaagac atagaaaaga tgattttctt tttactcata gtgtgaatgg
    37561 gtattcactt gctatggatt ttcccctcac aaaaggaaac agagagaaac tttggaaact
    37621 ttgctatgaa ttagatgaga tcgttatacg aaataaaggt agattttact tcgcaaaaga
    37681 cagcactctt cgtccagaag tggctgagaa gtttttatcg aaagatgcaa ttcaaaaatt
    37741 caaaaaatta aaagaaaaat ttgatccgaa ctttatttta caatcggatt tgtataaaag
    37801 agtctttggg tgataaagat ttgccaccga ggaacggaga cacagagata tggatataaa
    37861 tactagttca tacaatccag cagaggatga aattgtcgtt cgttttcttg gggaattaaa
    37921 aaaaagtatt ccccagtatc tgaaaaaagt aatactttat ggttcgcgag cacgcggaga
    37981 ttttagtaaa aattctgact atgatttttt agttattctc tctcagaaaa atttacaatt
    38041 aagcgattta gtatatgacg caggttactc tatattagat tcttatgaaa aattagcgtc
    38101 ttgtattatt tgggatgaaa aggaatggaa tcagaaaaaa gatttttctt ttgctaaaaa
    38161 tatttctcgt gatggaattt ctttgttatg aaaaatatca atggcaactg aaattttaaa
    38221 tacaatcagt gattatataa agaatcattc gaatgcaaat taaatatatc actccgatta
    38281 tttacgggtt aattcgtatt atccgtttat gaaaaaaatc tcatttgttc taatcctttg
    38341 ttttttgttt caagggtgtg cttctatttc tttctatatg attggaagtg gaaaatacga
    38401 agcgacgata gtgccacttt cacaaagaaa agattgcaca ttcgaagatt tcatggtttc
    38461 aaaaagcgag gcatatgcgt atcgatttgg agcaataggc gcgattattg atctcggatt
    38521 agtagttaat tattctctta aattgaatcc tgtaggtctg caattattcg gtgtttttat
    38581 gtataccaac cagcactgcg cttgtcactc ttttgaccgc tgttattcct acctttagaa
    38641 cacaagaggg ctggaaaaga tattctgaaa ttgaatgcaa agacgattat tttgttatta
    38701 tgtatgaggg ttgggaacga tttgaacaaa gtgggtctac attcttttta atgtctattc
    38761 taaattatcc gttcgattat gatatttccc aaaatgaaaa atttgcaaat ttccaaaaag
    38821 attttctaag aaacaataaa atccaaactg attctagagg aagtgaatac tagcgttaca
    38881 tctactaccc aggtgggcaa gcaaagttcg aagaagattt tgcaaagtat ctatataaac
    38941 ccaaacaata agctatacat tactactatt accgattgac aggattatcg ctatccgata
    39001 ctatgcatta tgattgaatc attcagggat aaagaaacag agaaggtttg gaaccaagag
    39061 ttttctaaaa aattagatcc acaaattcaa tctgtggctt tacggaaact ttttatggtc
    39121 gcaagagcca aaaacattga agatttaatg atccctcctt ctaataaatt agaaaaatta
    39181 aaaggagata gaaaagaaca gtatagtatt cgtatcaacg gacaatatcg aatttgcttc
    39241 ttttggaaga atgggaatgc ttataatgtg gaaattgttg attatcacta ggagttaata
    39301 tgaaaaaaat accaaatata catccaggcg aaattctctc tgaggaattt ttggttccta
    39361 tggggattac cgcatatcga cttgcaaagg aaacaaaaat caatcctact cgagtcagtg
    39421 aaatcatcaa cggcaaacga ggaatatccg cagatacagc tttgcgattt tccaagttct
    39481 ttggaaactc tgttgaattc tggatgggaa ttcaggatga atatgaaatt cgagaagaaa
    39541 gagtaaaaat agcaaagcag ctgagtggaa ttaagtccta caaagagctt gtattgacgt
    39601 aaataaacaa gaattaacta aattgaaaaa aatctcattt gttctaatcc tttgtttttt
    39661 gtttcaaggt tgtatatcga tggtagctat agaccaagtt aataccttca aaaatacaac
    39721 agaacatgtt cagaaggaga atgaggtctg taatttcgaa gaagtcttaa atcaaaagcc
    39781 aataaataga aagagatttt atgagaatag aattattctt ggaacctcta ctttggatat
    39841 gggagttagc gctttttatt atacaatgct aaatccaaaa gggtttatgc tattcgttac
    39901 aggattctat acctatcttt cttttggttt tttgttattg cagtttcgga gataaataag
    39961 gaatcagaac tagctataca attttcgggt tggaatcaaa tctatgcgga aaaatgttct
    40021 gaagattatt tcattttaaa atatgagaac gaagtcgagt ttaagcaaaa tgaattgaat
    40081 ataataaaag cagttcttca ttatccagtt gattacgata tccgacaaga tcgaagtttt
    40141 aacgaattta aaaaaacttt tctgcccaat ctcacaattc gaacctactc cagagagaat
    40201 gtgtattata gatatattta ttatacgggt ggcaaaggaa aattcgaaga ggattatgca
    40261 aagtatcttt atcgacaata ggaatttcga attgacaggc taataaaaga gttacaatta
    40321 taactatgct ggcagaaatc ctagaagatc cacttacatt tatccactca gagccagaac
    40381 cagatatatc gattatccgt agaagcgcag acgattacaa atcatcccat cctaatacag
    40441 cggagtttgt aattgaagta gcggtttcta gtctacgcat tgctagactc aaaatagaaa
    40501 tttatgcaga atcaaaaatt ccagagtatt ggattgtaaa tgtagaagaa aaaaatgtag
    40561 aggtctataa aacgccggaa ggacgagatt acaaagaaaa aaaagtgtat gcagtagcgg
    40621 atacgattca ttttccgcaa ttcctagcag gggaacttcc tgtagcagaa ttgtttgtat
    40681 aacaagtcta attagcatct ataattaggg tttgttattt tacattcggt gttgaatgaa
    40741 tcccctttca ttatctaaag attccaatat ttatccttta aacatagtta acacctacac
    40801 ctaattccct ttttctaaaa tcatctccgc taatactcta tgaccttctt tatttaaatg
    40861 agcgtcatac ggaaagtaaa gcttttctgg atctttatca aagaaatatt catttgtaaa
    40921 atagcatttt aactgattct ctttgcaaac cttctgaatt tgctgatagt gaggattctt
    40981 ttggctgtgt gcttcagatg agaaatcctt taaagctccg gtaattccgc cttttgcaaa
    41041 aaactctttg gctggaatat agagaaagga aagttctaca ttgttttgtt tgcagatatc
    41101 tagaatttga tttagataga tttcattttt tctaagacga ctcttccata attcttcttc
    41161 tgcatcttta taaaaaccaa attggtttcc gtattttaca ttgatatctt ttccaagagg
    41221 acttaatttt ccgaattttt gaatagattc ttcgtgactt aatgttttta cttttggttc
    41281 tctacctact ttgtaataaa gaataggaga gggttgtttt tggaaaaatt ttacgacttt
    41341 agtcgttagt cgatataaag ttctctcttt gaagaaatta taaatagatt ctccaaagat
    41401 acttgctcca agtctttgct ttggagaaac aacttttcct tctttgtcta gttctgtaaa
    41461 aatttcaatt ttgtcgttat cgtctagatc attgtcaaat aattggacaa ttactttctt
    41521 tggtttgaat gtttccaaat aatatttcat ttgaagagca aataaaattg gagaggaccc
    41581 atgaagacct agattaagga atttttcctc tttagagctt tgatttaaaa tttcagagta
    41641 gatttcatct gcttctactc cccagccttc tgtaaaagaa tctccaatga gaagtgttga
    41701 atatttatga ttcgaatcta ttttttgtct aaacccgttt tgatttgttt cgactaacac
    41761 tgcgtattcc tcagaaacta cttttccagt tttgtctgga tacatacgaa agagtaattt
    41821 tttatcaaag ccctttacta aaatttgttc tggatcaaac attcctagaa tgaactcaaa
    41881 aagacctaga ttgaaaagta tgattgcgag tagaaaaaat gttttcttca tagtaattgc
    41941 aattattttt taattgccat gaattgaata ctgaaaaatc aattgctgta tgaaacgaat
    42001 cgtaattcta aatcccaaaa gtagacatgg agcttcccgc gttgcctttg aaaaaatgcg
    42061 ccctaaacta gaagatcatt taggtttctt tgatatatat tttacaacgg ctccaaaaga
    42121 ttgcactcga aaagtgagag agattttaca taaaaaagaa tacgatcaaa ttttaatagc
    42181 agggggagac ggttctatca atgaggcagt caatggttat tttgatcatg gtcatttgat
    42241 tcaatcgaat atcccaatgg gagtcattaa tttgggaaca ggtggggatt ttgtaaaaac
    42301 tctgaacaag aggtcacagc attattttaa tgcactacga gataactctt atgagttagt
    42361 tgattgtggt gtaactacct tagagcatgg gaaggaacct catcatttta ttaatataac
    42421 atcaattggt atgggaggcg atatgaaccg acagatgaaa gcctcttcct tccaatatgg
    42481 aatggcagct tacttttacc atacactcag tgtcttattc aaatacactc ctgctaaatg
    42541 taaaattaaa atgaaacaac cgaatggaaa ttggctcgaa attgattcag aggttgtcaa
    42601 tttctttgtt tgcaatgccg gcttcagtgg tggcggaatg gtttgggctc caagtgcaag
    42661 cattgaagat ggagtttttg atatagttct agtatctaac gttccaaagt ataaattaat
    42721 tactgaatct cataaagtat attctggcaa gattgcatct atgactggag tgatggaatt
    42781 taaagcagtt gagattcatg ttattcctga gaggacttta tcacaagaga ttgatggaga
    42841 agtaagagaa atggattttt tacaaactca tgaatttcat tttaaattaa ttccaaaagc
    42901 gattcctatg gtaatataat tttatgagtt taaaaaaaat ctttgtttcg attcttgttt
    42961 tttattttct aattttttta attctacctc atacggaata tcaaccggct gatcccggta
    43021 tcaagatatt acagattaaa gattttattt tgtcaggata ttctaatttc tttgctagct
    43081 atcttggtag agaattagat ccatctttga cttttttccc aacgaagcct ccttttggat
    43141 atatagttgc ggataatcct tattatgtgt ttcctttttt tgtaactatt gcttatacac
    43201 ctttttattt acttggtggt atctatgcgc ttgacttact tagtttgatt tcaggactac
    43261 tgattgtatt tcttttgtat aagatatcca atcttttaga aatgagtgag acatttcaaa
    43321 aatcgatgat cctttttcta ggctttggtt ctattaatag tatttattcc tttatgttcg
    43381 gtgagtctat ccatgcttcc ctgtttataa catttgcatt ttatttgatt ttaaaaacta
    43441 gatcaactcc taactcattc tattttttaa ttgcaggatt gatttctggc ttatctgttt
    43501 tatttagaat cgaacttgct ttcttttgtt ttttattttt gctcggaatt tttattttcc
    43561 aaatgtataa aagtaaacta gatttaattt tactatgtgt tggatttggt atacctgcat
    43621 ttgttttgat catcgcaaat tataaagtaa caggtaatat tctaggttta cgtggaattg
    43681 aatttttaaa ttattctgga gcttcttacc caatcgagaa acgattattt aatctagtaa
    43741 aagctttatt tgtttctgat aagggattgg gactatttac tgcttggcct gtttttctgt
    43801 ttttaattcc ctatttttct aaatttaaaa tgtggaaaga aaaaaaagaa cttaacttca
    43861 ttctatttgt gtctatcgta tttagtttat tgattccatt gattgtaaaa aatgatgatg
    43921 gttcaatatt aggacctaga tttgcttcga gtgtccagcc acttttgata gttggaacat
    43981 tttattcgat ccaattacta gcagatcaat ctattttatc taaatggatt tcaaagttat
    44041 cttatttcat ttattattct attgcagtta ccattttagg ttatattatc ttgtttttct
    44101 ttttaaaagc atcccataaa atgaatattg agatagataa gaaaatttct ggagaagtta
    44161 tctttatcag taagccagat tattttagtt tggtttttcc ttctgtttac agaaagccaa
    44221 ttctatctgt tgattcagaa gaggagtttc ataaattctg taaaaataac agaacaaaaa
    44281 ttccaaatat gatatccatt gtgacttcca atccaaaata tgatatatca actgaattgg
    44341 agtataaatt agtagattca ttctcgagca atggaataca tgtttataat ttcaagtcag
    44401 cgaattagga atttctatga aacaattttt taaaaagcta tttcaatatt tcttattatt
    44461 tttactcatt cttgtttttc ttcgaatcat tgatttggtt tttattttta tgggtcaaga
    44521 gggaaaggct attttttatc ctcctaacgt tgtggctcat tctgattcaa aagattacta
    44581 ttatacattt aagacaaatt ctctagggat tcgaaatccc gaaatcggtg ataaaaaagg
    44641 taaacgaatt ttagttctag gtgattcttt tgtttttggc tggggagtaa atgaagaatt
    44701 tacctttgtt cgagttgctg aaaaaaaaat acaacaagtt gatccaaata tttctcttat
    44761 caatacagga attccaaata caggtcctga aaattatcta cctgtttatc gggctcttcg
    44821 agataaaata aaaccagaca tcgtgctcac agttttgtat ccaaatgatg tgaccgatag
    44881 tgggccgact gttttttttg aggcaattaa gacagcactt tcaaagaaaa aattgatccg
    44941 ttttgctttt tttgttatgc ctaacgcatc tgattatttt ttaaaatatt ttctagctag
    45001 attaaatcag ggaattaatt ctgatgcaga atctacttct cttagcaaac aagacaactc
    45061 acagccgcca cctccaaaag ttttgtcgcc ggaacaagtt ttagctaaaa aagaagaata
    45121 tagaaaaatg atctatcttc ttgctcagaa tatggatata aaaaaagatc gagttgattt
    45181 gtggcttaca gaaatagggg atgagaattt gacattagcc gcaaagggaa aaattagtcc
    45241 caaaattcta cttacaggtt tactctctcc tgatttttat gaagaaaatt tagatctaaa
    45301 aaagaagaaa atcgatcgat ttgctagaat gttagaattg gttgagaaaa ttagagtaga
    45361 agcatctgag agaaaagaaa aatttgcctt ggtgtatatt ccctctgaac ttcaatatga
    45421 tcctaaaaag acagatttac ataaaaagtt aggcgtaact acaaaggaag attggctttc
    45481 aaaaacatcg aatcttgaat tagaattaca aaagtataca aatcaaaatc aaatcgcata
    45541 tttaaattta acagaaactt ttagaaaaga atctaaacaa aaagagctaa acttcccatt
    45601 cgatttgcat ttaaatgaag cgggaaatgc agtcgtagta gatcctttgg ttgagtttct
    45661 aaaaacaatt gataaaggta attagtttga atattcagga taaaataaaa acttctccta
    45721 ttgcatctat tgttttggtt ggagttgttt tcttttatct tattttcttc tcatctagta
    45781 agggcgttca atatggtgat tttgggctca tgtatataca ggtgaaggat atagttgaga
    45841 gtggatattc tacatttgct gttagatata aaggagagaa agctgaccct gatcataaat
    45901 attttcccta taccaagccg tttattggca aggttggaca gaaatattat attgatttcc
    45961 ctccttattt ccctttatta aataccccat tttatcaata ctttggtatg aatggaattt
    46021 atattctaag ttatctagcc cttttaggaa cgttatatct aatatataaa ataggtgctt
    46081 tatacgaatt aagctttgag atgattcatc tttctatttt tctttattca tttggaatga
    46141 ctgcgactat gtataattta gtctttcatg aatatccaat tgcgattttt cttataacat
    46201 ctagttttta ttttggagcc ctttactata cgagaaaaaa taattattca ttggtatggt
    46261 ttggtttatt gggagggctc tctttatttt ttaggttaga gatgattttt gtgatacttt
    46321 cgattggaat tagtttggtt ctgactaatc caaaggattt ttttaaaatt ggatttttat
    46381 cattcttagg atttattttt ccatttatag cacttctttc tttgaatcaa tatatacatg
    46441 gtcatcctct tgggctaaga tatatactca cactgacaga agattctagt attagtctat
    46501 tggggcgaat cgatattatc cgtgatatgc ttttttcttc tacacgcggt tttttctttc
    46561 aatcttcctt tgtgatcatt cttcctttta tattattttt gagaaaattg atcttgtttc
    46621 aggaaagatt tttgttttta ttattattct ttggctttgg ttttatttta ctgactgctc
    46681 ccaatcatgg agatcataga gcacctcgct atttattcgg tctctaccct atcttagttt
    46741 tactagtagt catttccttt tcaagttata taaaagctac gtcttttgag ggaaaatcgg
    46801 tttttcaaaa accaattcaa attttgtttt ccattttagt attaatctcc tttctcgcaa
    46861 caattcaaaa ttatagatgg atgcaaaaag ctaccgtaaa tgtgatcagc ttgaatgaaa
    46921 cgatttctaa tttagccgat tctgaaacgg caattatatt tagagattat gcacaaccct
    46981 tgaatactca gaagatctat ttagaaagaa ccgtctttgt gtcggaaaaa ctagaaaatt
    47041 tgtcggaact aatacataaa attagggaaa gtggtttcaa aaagattctg gtgagtcagg
    47101 tctattcggc tcctttaaac gaagaagcca aagaaaaaat ttttcgagat tatattacaa
    47161 gtttatccct ttcaaaaatc agtgaatcta gttacgaaat aggaaatgag acagaaaaag
    47221 gattcgtgtc tcctagatgg attcagtttg gaaaaaatca gaatcttttt atattaaatc
    47281 tgtctttctt gaaatgattt ttctaagcta ttgagagctt cttgtcgtta atctatttag
    47341 aaaactttac gaaaatattt ttttagcaca agaataaaat tttgaatgac agaaatctat
    47401 tgattatgtc acatatacaa ttgccgtaag tcttattcga tcatcttgaa aatgtctctg
    47461 gcaatttatt acccatcata cgggagtttt attagtgcga aagtctttaa aaattattaa
    47521 tagggagaag ctttaattat gggagatggc tcactttcac aagatgaaat agatgcacta
    47581 ttacaaggag cggacgaaac accttcttac gagacaagcg gttcaagtgc gggaatgggt
    47641 ggtgctgggg atgacctttc tcccctcgat agagatgctt taactgagat actaactgct
    47701 gcattttcgc aggcaggcgc tatgcttgga actctattgt ctaaaaatgt taaatttgtt
    47761 aatccaacac cagaatcaaa aagtgcagct gatttaagtg cagaaattgg tgcaggatct
    47821 gtttgtttat tttcaacctt aagtggtccg gtaaatggta gagcttgtct agtaatgtcc
    47881 atggagaacg gatccaaaat tgcccatctt ttaatgggtg gtctttccac tggagcaatg
    47941 gagactgcac agttagaaac tctaaaacaa ggttttacat ctgttattgg aaatgtaaca
    48001 gtccaaattg gaaccaaggt atcagctcct attactggtt ctcccgtaga tgtttctagt
    48061 tcaaaatctt cgagagattt aattcttcca gatggtaaaa ctttagttcg cacaacttta
    48121 aatttaaata tagatgggat cggggttttc aaggttcatt atatcatttc aaagagtatg
    48181 gcagatgaaa ttcttgcatt gagccgtagg actccaaatc gtccaaacta caatgatggg
    48241 ggtggtatga gtgtgaatgt tcctgcaaat aacttcggaa atcctgttca gggaatggga
    48301 atgtcttctc ctggacctca aattggaata aaaggtgtaa gttttccttc tctttctaca
    48361 gcaggagccc cttctggcgc aaccaatctg aacttattaa tggatgttca gatgtctctc
    48421 acagtagaac tgggtagaac taaaatgtat attaaagata ttttagggct tggtgagggt
    48481 tcgatcattg agttggataa acttgccggt gaaccagtcg atttattagt gaacggaaaa
    48541 ttaatagcaa aaggtgaggt agtagtgatt gatgaaaact tcggagtgcg tgtaacagat
    48601 atcgttagtc cgacagatag aatcaaaaac gaaagtaaat aaatgatctc aagatttatt
    48661 tcttttactc ttttagaaat gtttgccaga gttttaaaat ttcttatttt actattagtc
    48721 ttgcagacat ttttaattgc tggtgaaaga gatgaaatgg atcaacttct ccaaaaagaa
    48781 ttgggacaag agccaacaaa gcagctgact gaagacaaca aaaagccaac cgacaaaaaa
    48841 aatgagccta taaatcctgt agaagaaaga tatagaagag aggatgactc ttctagttta
    48901 ctttggacgt tagtgaaagt cttttttgtt tttggtatac ttacagctgc tatgtattat
    48961 gttctgaaat ttgtttcaaa aaatagacag agtatgtatc cggtccgaga tgcgatgcga
    49021 gttcttgcga gtttgccatt agcccctaat aaacaaatac agattgtgga tgtatctggt
    49081 attcttcttg tgattggtgt atcggatggg tcggtaaatt tgattaaaga gattgattct
    49141 tctgaaatta aggaaaaaat ttatcattcg aaagaaacag ttcagccacc aacagaaaat
    49201 ttcttagaag ttctgctctc tacttttaag aatttagatt tcaaagcatc gcataacaca
    49261 tcacataaag atacagaagt gcgtgatgag gaaattatga atgaaattaa atttcgtcag
    49321 ttggaaagat tggaaaaatt aaaaaaagaa agatcagatc tctcaggagg caaggataaa
    49381 gatatatccg attctttttc ctaatctaaa atgcgacata ataaaactat gatatcattt
    49441 cttactctct ggaagaggac tttgaaagga atgaaacaag gcaaatccct atttacaaag
    49501 cgtttggttc taagattagc atccatttcc attttagctg taatcatttc tatttcaagc
    49561 atagagattt acgcgcagag cacgaatact agaattccga ttccaaattt aagttttaat
    49621 gtaaatgaag caaaaaatcc gaaagagaca agcctctctt taatgatttt gtttttagtt
    49681 acaattttat ctctagcacc tgcgatcata atgtctgtta cctcctttac aaaaatcgta
    49741 atagtaatgg attttgtgag aagagcgtta gccgttcaga acttacctcc gaatcaagtt
    49801 atggttggac tggcaatctt tatgactttt tttattatgg ctcccactct gggaaccatc
    49861 aatgacaaag ctcttactcc ttatctagat gggaagattg agacaaatac tttctttgaa
    49921 agatcaatgg tccctcttcg tgagtttatg atgcgtcaga tcggaaagtc tgggacaaaa
    49981 gatgtagctt tatttttgaa attaggtaaa gtaaaacaag ttaaatcttt tgaggatgtc
    50041 ccttcctatg ttctaatacc agcctttatg ctctcagagt taaagaaggc atttataata
    50101 ggtatctatc tctttattcc atttataatt attgatattg tagttgcatc aacgttactc
    50161 tccatgggtt taatgatgtt acccccggta atgattagtt tgccttttaa gttgatttta
    50221 tttgtgttag tagatggttg gaatttatta gtatttgaat tagtaaggag ttataaatga
    50281 cggaagtaga cgtagttagc ttgattcgag aagctttatt tattactata aaaatttcgg
    50341 ctcctattct agttacagca ttgattgttg gtttaatact cggtattatt caaacaacaa
    50401 catcgattca agaaccgact atcgcatttg ttcctaggct catcgcaatt tttataatta
    50461 taattatatt tgcttcttgg atggttcgaa ccatgaccga ttacacaaga aacattttta
    50521 tgatgattga gagaatatga tagaaacatt cgtaaacaac ttccagacgt ttatgatgat
    50581 atttgctcgt attatggggc tatttagtct tgctcctatt tactcttctg aggctgttac
    50641 atttcaaatt agagtgatta ttgcatttct tctctctatg attttgtttc ctgtaaccgc
    50701 taattattta catgcagttc cgactggtat gggagaatat gctcttatta ttgcatcgga
    50761 agctttaatt ggagttctaa ttggttttat tataagtatc atttttgctg gctttcaaat
    50821 ggctggtgag tattttagtg tgcaaatggg gtttggatat acagaggttt tagatcctgt
    50881 cagccaaaca tcgctacctg ttatcagcac attgaaaaac ctatttggaa tgcttgtatt
    50941 tctgactgtt ggggctcatc gaactatgtt agaaagtgtt gccttctcct tccaaaaaat
    51001 tcaattatta accttcacaa gagaagtaaa tattggactt ttaaaggctt ttgaatatgc
    51061 tatcggcgcc atgtttttgg ttgcgtttaa aatttcatta cccgtcttag ggatattgat
    51121 tttggttacg atcgcagagg caattatggg aaaggcagcc cctcaaatga atatcatgca
    51181 attaagcttt cctgctaaga tatttgtagg acttatggta ttaattatta taattccttt
    51241 tattgataaa caaatggaag caagttttac cttgaccttt gacagattaa atacgttaat
    51301 gagtgagtgg ccacagaaat gagttttttg gatagcattc attcaaatca gatagactac
    51361 gatttgtctt tttatcgaat tgatttacaa ttatttgccg cagaagatga aggtagaaca
    51421 gaagagggaa gtgagcgaag acgaagggag gaaagagaca aaggaaatgt tcctaagtca
    51481 aatgaacttc ctgcagcttt agtattggtt gcttctgtca tagctctgta tctacttggg
    51541 aattatattt ttgttcgatc gttagtttta tttaaaaaat actttcaaga ctttgctaag
    51601 agatctgatt ttagcactga agaatttgga attcttttga agtccgcttc agccgatatt
    51661 gcatccttac tttttccaat acttggcata acctttgtag cagctatcgt tggcaacgtt
    51721 gttcaagtag gatttttatt ttctcctgga gcagtgggtt ttaattttaa taaaattgtc
    51781 cctaaatttg gaaaagtttt accaaccaaa aatacaatct ataatttaat taaatcaatt
    51841 ggaaaagtta tcatcattgg ttgggtgagt tatgtaatta tatcaatgga ttttttgcca
    51901 attcttctaa ctggtgatat gggtttaaag agtgctctgc gattgctggc aatttcctcc
    51961 gttaaaatct ttctagttgt aggtgtcatt ttatttgcac tgagtattta tgattatttt
    52021 tatcagaaag ctgaatttga agattctatc aagatgactc cctccgaagc aaagcaggaa
    52081 atgaaagaaa gtgaagggga tcggtcttta ctgaatcgta gaaggcaaat ggttagagat
    52141 tttattcgta ggggtatgtt gcaaagagtt ccaaaggcag atgttgtgat tgtaaatccg
    52201 acccactatt ccgttgcact tgtttatgat gctaaagttc actccgcacc aatggtaact
    52261 gctaaaggaa tagatgaatt agctcttatg attcgtcgtc tagctaaaaa gcataacgtt
    52321 ccaattgtag aagatcgttt acaggcaaga ttgttatatg atgaagtcga tgtggatcaa
    52381 gaaattcctt ctaagttttt tagagcagtt tctattatca tatccagatt agataaattt
    52441 aggagagtcg cttaatgagt gagaaaaaat ggtataacca atccgatgta gttatggggg
    52501 tcggagtcat tgcgattgtt gcaatgttga tcatacctct acctggattc atactggatt
    52561 tattaattgt agtcagtctt gcggctggac ttatgattct tcttacatct ttatctgtaa
    52621 aagaagcatc agacttttca atatttccaa atttgctttt aatctcaaca ttgtttcgtt
    52681 tagcacttaa cgtttctaca acaagacaaa tcttatcaca gggagctagt tttaatagtc
    52741 acatcattga agcatttgga actttcgttg tgggtggtgg aacagggctt gggaagtatg
    52801 tagttggttt tattatcttt ttgattttaa caattgttca gattttagtt attacaaagg
    52861 gtgcgactcg tatttcagaa gtagccgcta ggtttacttt ggatgcgtta cctggtaagc
    52921 agatggcgat tgatactgag ttatcaagtg gaaatatcaa tgaagaggaa gcaagaaaaa
    52981 gaagaaaaaa gattcaaaga gaagtagatt tttatggggc aatggatgga gcgagtaaat
    53041 tcgtccaggg ggatgttagg gcagggctta taattaccgc aatcaacctt ctcggtggaa
    53101 ttattattgg tgtatctatc cgtggagaaa gttttgtttc tgctattgaa acatacggca
    53161 agtttacaat tggagacgga cttgtatctc aaattcctgg tctcttatct acaacagcaa
    53221 ctggtatgat tgtaactcga gctggttcag aatcagaact tgcagaagag ttcaaagcgc
    53281 aattatttaa taatccaaaa acattatacg ttgtttctgg gagtttgggt ctagcatctt
    53341 taatccctgg gttgccattt ttttctcttt tgcttttttc tctatgtttt gcctatttag
    53401 catactcgat tgagcagaat gcaaaagagt cgttagctgc tattgaaaga aaggctggag
    53461 aaagtaaagc tgagaaaaag ccagactatt acaaagagtt acgcaccgat ccaatcgaag
    53521 tagaacttgg tttgaattta gttccattag tggatactaa tcagggagga gtattactcg
    53581 atcaaatttc gaatttacgt agaagatttg cgattgatac aggacttgtt attcctgctg
    53641 tgcgaatttt agacaatctt gaattgcatc aggattctta tgcaattaag atacatggag
    53701 ttgttgttgg agagagtaaa atccgaccag atcgtctgat ggctttggac aatgcaaaga
    53761 aagtgactga acctgttaag ggagaagact ttatcgagcc tacatttggc tataaagcaa
    53821 aatggattga tcctgctgat aaagtggatg cagagaataa gggatataca gtcgttgatg
    53881 cgtcaaccgt tatagtaact catttaaaag aattaatttc taatcatgca gcgactattc
    53941 ttggaagaga agaggttaaa agtttacttg aacatcagaa aacaactcat cctactctca
    54001 tttctgaact cgaagataaa aaagtcggga ttggacttat tcaacaagta ttgcaaaatc
    54061 ttcttaaaga aggtttgggt attcgtaatt taagcacaat tttagaatcg attgcaaaca
    54121 atgagtctag atcaagggat ccattttttc tttcagaaaa tgtaaggcag gctatttcta
    54181 aacagattgt gattgattat ttaagttcag atggtaaact tcatgtaatt acattagatc
    54241 caagagtaac ggatcgattg agtaaaggta tagtggttga tccgattgag ggaagcttaa
    54301 tttcgatccc acctgattat tacaataaac taatagaagc tgctgcgcgg gaatacaatc
    54361 gatgtcacag cgagggtaaa tttccaattt ttgtagtaag cagacctatt agaatgccac
    54421 tatcgtatat gtttgcaaaa gaatttccac ctagaaattt tgcagtgctt gctattgagg
    54481 aaattcagtc ttcaactaag actgttatgg atggagtgat caatgttcaa tccaatgaag
    54541 gtgttagggt tgcaacaaac agaggttaag agagaaagtc aaattatctt ttgattgtaa
    54601 tcggataagt atgcaatgat tgtaaagagg tagaaccatg gattttgtaa aaataaaagg
    54661 aaaagatata caagactgtt ttatgcaaat gaaaatgaag tatggaccag aagctcatgt
    54721 ttataaccaa agagtggtta tagaaggagg catcatggga actaaacttt tatctaaaaa
    54781 attctacgaa attgaaatcg gaattcctga acaacaaaca tcgaaagata gagttgagaa
    54841 aaaactacag gatttaaaag aattattaag acaaaaatct gaagatgaca caagaaaaaa
    54901 aagcattcac gatttaaagc cattactgca agatgagaaa tcttattctg tatttaaatc
    54961 tctagaaaaa gaagaagatg cgatagttga tacaaatcag aaccagagta agaatcttgg
    55021 cttatcaatt aaagacgaga ttacaaaaaa ggattctgga aaaaacaatg caaatgaatc
    55081 aacctattta aaacgtttga aggaaagatt aatttcagaa ggaatgacat tagatttttt
    55141 acaagagttg attattaaaa cagataaaca tttatcatca attgataaag aaaaaccttc
    55201 tgctgtaaac gataagtttg ctggaatact agaagaccgt attagtgtag attcagactt
    55261 attcacagga acatctcgtg gcaagagaaa agtaattttt tttattggac cgacaggaag
    55321 tggaaagact actactgttg cgaaattagc agctaagtat tttttacata tgggaagaat
    55381 ggtttctctc tatacaacag ataattacaa gatagccgct attgaacaat tgaaacgtta
    55441 tgcggataca atggacatcc cgttctatcc agtgaaagat gttaaacgtt tacaggagca
    55501 attgcttcga gatgggtctg aattaattct agtagatacg cctggttata atcataaaaa
    55561 ccaagatttt gcaaatcgaa tgaagcaata taaagatgca ttcagcgaaa aggatcagat
    55621 agaaaatatt ctaatattac cggcaacatc ttcgtatagc aatatgaaat ctgtcattgc
    55681 agcatacgaa agtataggat ttaagagaat aatcttgaca aaaatagatg aagcggatta
    55741 tgtaagtcct gtacttgaac tggcggatct aaacaataaa tcctttgctt actttagttt
    55801 gggacaagaa gttccgtttg atatagtagc agctagtaaa aagacattgt ctgaaatagt
    55861 tgtctatcca gacaagatta aagatttaaa aggggataca gtcgcaacgg gctaatccca
    55921 aaaaaggaaa acaatgcagc caaaccaaga tgttttacct aatgacgaat ttcctgcaca
    55981 ggaatctgac aaaagaggta gtaagattat tgctatttct tctggaaaag gaggcgttgg
    56041 taagactgtg tttacggtca acttggctgt ttctcttgca aaagcaggaa aaaaagttct
    56101 agttatcgat ggagatcttg gactagggaa tgtaaatgta ttgtttggat ttattcctaa
    56161 gtttaattta taccatgtta tgaaagggca tcgttccatt cgggacattg tatttcatac
    56221 tcctgaaggc gttgatgtta ttcctggttc caacggttat cctcaatttg caaatttaga
    56281 tgataaatct agagatcaat tgattcaaag tttttctcag ctagacgctt atgattatgt
    56341 gttagttgat acgggcgcag ggattggtcc caatgtgata ggttttacgt tgtatgcgga
    56401 tgaagtcatt ctagttgtag ccccagatcc atcgtctgta acagatgcct atggattgat
    56461 aaagtctctc atctctctag ataaaaaaaa gaaaattagt ttcattgtta ataaaatccg
    56521 atccgaagcg gaaggcaaaa aggttgtaaa gagaattaca gagattagta ataaattcct
    56581 tgggatcaaa ccaattagta ttggcttttt ataccaagat gaggatgttg atcgtagtgt
    56641 aaggaaacag aaaccatttt tattatttgc tcctcgttgt aaagctgccg aaaatctata
    56701 cgagataaca ggaacaatcg caaatattcc gattgatcca tatcagacac aaccaaagat
    56761 gatggtgtat ttcaaaaagt ttctaagctc tttgattccg aatacctagg aggattctct
    56821 gcaagttaaa tttataatcg tattttttgc catcgcttta ctggttagta tttcttgcgg
    56881 tgtcattact ggaaatagaa tcttgcatat cgtttttgta tcaggtattg gttcattgat
    56941 ttttggaggt ctaggtttag gaatttatat ggttctcgaa aataaagttc ctgaattttt
    57001 agattttctg gcggaagtag gtggtggaat cggatttcgt agagctgctt cgagtgatcc
    57061 tatgggaatg ggcggcaatt actcctcaga caatgaaatt aaattaacca aaagtgatga
    57121 atcaatgagt agtgattttg gtggtggcga tatttcatca ggcaaaacca aagatggaaa
    57181 gtttggagat catattatca tcaataacat tccaattaaa aatgagccaa aactcatggc
    57241 agaagcaata cgaacaatca tgtcgcaaga cgacggagaa actcccaaac aagcctaatc
    57301 caagtgtata cattcgtctg gtttaagagg agttttgtct ttataaaaca cttcttacca
    57361 tttgcatcct tgatatagtt caattcaaaa gtctaaaaaa ttttatcatt ctatataaga
    57421 cgcgattacc ataccacttc cagttgcagt gcttccagcg attgtgctga tcaaagaaag
    57481 aattccatct gcattgattt gataggatct agtattgatt gagtcaccaa taaaaagaaa
    57541 tcttccactt ggatcggaaa tcacagatgc gggactattt aatccagtta caacagttga
    57601 tgaagaagtt aatattcctg aagaatttaa atcaacttta tatgctgtat ttgctgtgga
    57661 agaaacggca tacagccatt tgcctgattt ttcaattgcc attcctctaa gtccagaagg
    57721 ggcaacggca aaaaaaccca aactacctaa ggcgcctgtc gtttgattta cgctgaatgc
    57781 atagatagat gttccagttg ccaaatacac tatatctaat gttggatgag agaccataaa
    57841 gttttgggat aatggcaagc cagtattttg aacagaagaa agaatttgac cattcgatga
    57901 atttaatgca tatgaatata aatttgaatc gcctgaagaa cgaagcacaa acgcaaactt
    57961 tccagttcta tctaaaaaaa tggaagatgt aactgtaaca cctacattaa aagaagaatt
    58021 ttgaacaaga gttagaattg ctgtagatgg attgatacta aaagatgaaa gttcaaagct
    58081 tccagcattg gatgcggaag catataaaaa agaaccaaca ctgtccactg tagctccttg
    58141 aatactaata taattcgtaa atgttgcagt ttgaagcgag ccatctgtgc tattaatgat
    58201 tctaggaagg attgcatttc cagcagcgca aaataggaat ttgccggaag agtcaatgga
    58261 cacagaacgg acagtcccac taccgactac ataggatgtg attggattta aaactccagc
    58321 agctgaaatg ctaaaatact ttaaattagc agaggaatca tagacatata gatacttacc
    58381 acttttttca aaatttttaa ttagcacaga aacaggagtc ggattttctc cactggaatt
    58441 tgaagagtaa gcgagtatat gtgttacgcc tgacggtatg gttgtgttgt cgctcaattg
    58501 aaacgatagg tctgcacctg tttttgggag agagtttaga atcgtcgaat tatatttaga
    58561 tgttcccgat gttccaaaat acaaattata tttttcaatg tcagattcat ctaccgcctt
    58621 agtgacactt attgttcctc cgatttttcc aaaatttaaa atatcggtat ctataaagtt
    58681 taccgacgcc ggtggattgc ggggagtatt ctgagagaga ttggaattta ttaaaccttg
    58741 aatcaaattg atagcaggaa agaatctaaa tgtatttcca atatcataag gtgatttttt
    58801 agcttttaag cagttgatag aagcaaaaat tagaatgcaa attcctacag aagtaatttt
    58861 tttatttaaa tcttgtttca ttttcaaagc tctcctaatt tttcaaataa ctcaattcta
    58921 ttgttctgta agtgacagtc ttgttacttc actctctgtt tttctaaact gtgtaatgat
    58981 ttgacttcga ctcattcgat tgcgaaagtc tgtttctgta ttagtagact taatttttga
    59041 aaaacatagt gtttatcaaa aaagtcaagg gaaatttgta aaacttggtg gctataatta
    59101 aaatctggag ctcttggtta taactgaatt ttatctagaa tatttgggct ctttaaaaat
    59161 tcttcattca aatttttaag agagcctatt ttactttaga ttcaccaaaa aaacaataga
    59221 tagcagatga caaaactaat ttcccgaggg atttcattta cagaaccgaa ttattatctt
    59281 atctatttac ttttcatttg attcactgat tggtatttga attaaaaccg ttgttccctt
    59341 ctctatctca ctctctaaaa caatttttcc attgtgtaat tctataattt ttcgagcgat
    59401 aaataatccc actcctactc cactcacttc ataggtgaga gaagaatcta ctcgaaagaa
    59461 tttttcaaaa actttttctt ggtgctcctt cgctatccct atacctgtat ccgtaatttg
    59521 aatttgtatc cctgctgatt cgctccctga gcggagccga agggagctct gtcctattga
    59581 tatttcaata ctgccacccg atttattata tacgattgca tttttcatta ctgcggatat
    59641 tgctttttct aaaagaatgg aatcaccact taaattcatt ttgttaggta aatttaattt
    59701 tagattaatt tctttttctg ccatgagcgg cttatatgta tttaccacac gatttatcaa
    59761 ttctactgaa tctaaatcag agttttgcaa ctgtaaattg gattctatat cagaaattaa
    59821 aattaaatca ctcacatatt catttaactt ctgtgcacta tcgtatatct tattggaata
    59881 tttttttatc ttgtccggaa aatcttttcc attgtttaac atttctgcgt atgcatagat
    59941 tacggacaat ggtgttttta tctcatgaga tagattagat aaaaactcat cttttacctt
    60001 actcacaagt gcaatttcgt ttgtcttatc ctttagtttt atatgcgttt ctactcgtga
    60061 aagcaattct actttcgaaa atggtttcac aacataatca tttccttccg cttcaaatcc
    60121 ttccagtaaa tcactcgcat tgttttttgc agttaaaaac aagactggaa gttgattcat
    60181 gtcatacttt tcccgaatct tttcgcatac ctcaaatcct gtcatattag gcatcataac
    60241 atctaacaat acaagatctg gtttttcttt ttctaagtat tctagggctt cttttccacc
    60301 aaccattgtt gttactttgt atccacgcaa cgaaaaatga tttgatatca cttgtaaatt
    60361 tacaggctca tcatcaacta cgagtatatg tgcattagcc ttagtttttg attcattttc
    60421 ttctattgca ttcccttctt ctaaagattg atgaattggt gtcatagtgg acttttcaat
    60481 tttatgcggc atactatcag taaccggtat tgtaaagtaa aatttagagc ctttacccaa
    60541 ttctgattct acccaaatat ttccaccttg cagttcaact agttgtttgg taacggataa
    60601 tcctaaacct gttcctccat actttcttgt attggatgca tctacttgct caaaggattc
    60661 aaatactttt tctaatttgt cttttggaat tccaatccca gtatctgcga tttgaatttc
    60721 taaaacttgt gagttttcct ttaaattggc actcactgtc acagatcctt cttcggtgaa
    60781 cttgatagca ttccctatta cattgtgtaa gatttgctgt agtctatttt cgtcagcaag
    60841 cacagcggtc gttgattctg gaatttgatt gatcaattta agatttttct tttttaccaa
    60901 aggctctgat aattgaatca ctagatttgt aatagaatat atatctacag gttgtaaatt
    60961 tacctgtaat tctttgtgtt ttaattttga aaagtctaag atatcattga taagattaga
    61021 tagccgtcta ccgctagata tgattaaatt taaattagct ttagcttctt ttgattgctc
    61081 ccctgcgact ccatctacta acgactcggc taatccgatt ataccattta gaggtgttct
    61141 tagttcgtgt gatgtgtttg ctagaaactc atctttttgg cgatccagat cttgtaattt
    61201 atgattttgc tcttctaact ttcgattctg tgattccata gattgagaaa gagtttctac
    61261 ttgggtaaat gcattcgtaa atctcttaga tagaaaaaag gcctgtgaga atacaaaaaa
    61321 caaaaatcca aagggagcaa aatgccctgt tcgaatcact ccgtttgaaa agagtaaatc
    61381 atttactatg gtaattgtaa atacgaaaaa tccaaataca aaggtaattg caccaaatct
    61441 cttgcggtaa acggctaacg acaaggtata gattgaagtt agtaaagcaa tgacaataac
    61501 cacatgcatg aatgggagat atttagtaaa aacaattggc tcaaaaagga taattgtcaa
    61561 agatagtaag aggggactta gtattatccc tttgcgaagc caatttggaa tttcagaagg
    61621 aaacactttg gcaacaaatt ctataataat cggaacgcct aaataaagaa taagatattc
    61681 tatttttatt gctaagaacc aagaaaaacc aggaaataac tgaacccaaa ttttttctcc
    61741 ggtcattaaa cttcgtatcg aaataaataa acaaaacaat ccaaataaga gagtagtaga
    61801 gtctttggtt ctaagtaaaa acaatcctaa atggtaaaat cccattagaa acaaagcacc
    61861 aaagataaat aaatcgaggc taatactgtt ttcgcgcatc ctttgtaaat cttggattgt
    61921 cccaaaccaa gtaaatcccc aaagtcctcc tttatcatga ctataattcg aaacatgaaa
    61981 tacaaactct actgaatctt ttaaatctgg taatacaata atatgtggtc taaattcggg
    62041 attagcctcc tccgcactct ttccgatttt tccaacctcg gtgagtagtt ttccatctac
    62101 ataaaaccga aaggcgctac tggcagtgga taatctaatc gcaagagtat ttcgtttact
    62161 ttcttctgca ctgggtagta atactgttag ccgataggtt gtaaatcctg tagagccaat
    62221 gggttttcca tctacttgaa aattattcca tactccgggt ttattgatat atttcttttg
    62281 ctcaggcgaa atattttttt cgatttctgg atttcctggt tctaaaaact ccatccaata
    62341 gaactcccat tcaccttgca aattcactac tccatcttca gaaaaattcc atcccttttt
    62401 tccgtctggc aaggaacgta aatctaaaac tccctttatt gcctttggag attttcttgt
    62461 atttcctttt tgacatgata caagcgatac aatcattaat atatataaag ctatccgcat
    62521 actatttcct ctcctataaa ttctggtaat gttataaaaa aacttgttcc ttcccctaac
    62581 tttgtctcat atgtcaaacg accgttatgc gcttctacaa tcatcttaca cataaagagt
    62641 ccaagtcccg ttccatttgc tttcccttcc gtggcaaatg cctgaaaaat tcttgtctgc
    62701 acggattctg gaattcctga accgttgtct gtaagagaaa tagttaagcc ttctcgtgcc
    62761 gagacgcccg cgtttcctaa agggggattc tgtgggattg attctactcg aattttaaat
    62821 ttgtattttt tactaccatc ttgcattgct tcaattgcat tctttgcgag gttaacaatg
    62881 actcgccgca ttcttccttt atcgatttgt gcgaatcctt catattcaat ttctgaacta
    62941 taatcgattc ctgaatattt aaaatctatc gaaagaaatt gctctatttc taaaataaaa
    63001 tcttttatgt ttacatcttc tagattcaaa tgcaattcat cttttgaaaa atccaatatc
    63061 tcgtatgcta gatcattgag acgatccatc tcacgaataa ttatattcaa gttcttttct
    63121 ctttttttgg gcgttattgt ttctgaattc gacatttctg ccaaagcttt tattgtcgcc
    63181 attggatttt ttatatcatg cacaattcct gccgccattg tgcctacaaa tgagagtttc
    63241 tttctcgatt cagctaacgc ttcaatttct tttcgctttg cttcactgtc tacatacatt
    63301 tttcctaact gatctagcat ttcatttaat tgctctgttc tcactaaaac ctttttttct
    63361 aattcgttat ttaatttttc gagttctttt tgattctcta tctggataat ttcatttttc
    63421 ttttttagtt cattaattcg atcagcaaga ccaagtgata ataaaattac ttcaaaggaa
    63481 caaccaagga taaatgattt ctcagtaaaa aaattcgaag gtataatgga agaaattttt
    63541 aaaagcaata acattccacc ggaaattaga aagctccatg caagtaggta gtatttggct
    63601 gttcgagatc cttttcgcaa gcattgaatt gcagcaaata aaattattat acagagaata
    63661 aattgagtta gcattataat cctccatatt gcagcatagc caatcacaaa acttaaaaca
    63721 agataggatg aataaagaaa taagattaat cttaaaataa aatctaaacg tgggattaaa
    63781 tccctgctcg aaagaaatga cctagtaaat aagactaaca tgattacgaa aagaatattt
    63841 gttagagtaa tactctgatt tgcccaccaa gaattatctt gccataaata tttataagcg
    63901 tacccgtagt agataaagac ccagaataaa taaaagaata gatagaggat ataatatagg
    63961 taggttttat cgcgaacact tagaaaaata aaaaaattat aaaacatcat aacagaaata
    64021 attccaacat aaatcccaaa gaaggtatca ttaattgagg atgttttgat atatgtatct
    64081 ttattgtata aagttaaagg tattgattcg tgaagtccat caaaagacca agtttttaaa
    64141 taaataaaac gtgaattatt tggattcact tcaactttaa ataaatagtt tttattatca
    64201 tattgcctcg aatgaaaaag gcgtctgtct ccagtttgaa tgagagtgta tttgccatta
    64261 tcctccatat gataataatc aatataatca atgggaggaa atgctatatc caaaagccaa
    64321 tcttcttttg attttaattt attttctatt ttaaatcgta accagaaaac ggacttcgta
    64381 aagccaaaac tcggaacttc tttgtcgctt ctcacccata atttttccat ctctggtttt
    64441 tgcacatctt ctattgtaag ttttccttct ttgtcttcta aaatttctag gtaaagtcca
    64501 agcggatact cacccttatc ctctgtaaga acaactggct ctatcgcata tatggatgaa
    64561 taaaaagtaa accaggcaat aagaaatatg aaaatcttgc cataaacagt tttatttttt
    64621 aatatacttt gattctttat tttcatttga tttgctcatt aattatattt atttctagcg
    64681 gttatcgtta ttaatttatc ctgactactg taaaaagtaa aaaaagactt agctgtagtt
    64741 aagtagatca aagaataatc tcttattaaa atttctttga ctcaggtaat agttctttta
    64801 attgatttaa aaattcatta tacgattctt tgtttggaaa atacttttct ctgaggatga
    64861 taattccttt tttggtaaag tatagttcaa tcaaatcttg ttccctattt acttcgccta
    64921 tttcttccca taaataattt accgttatag atttagagga agattctatt ccatttttat
    64981 tgaggcttat tctggtttca ccatcttgaa taaattcttc ttttataaaa ttgctcactc
    65041 ttcttttcca aatccatgga gtgaaaaagg aatacaggaa atttaaaaat aaaatgaata
    65101 ttccaaacaa atagaggtag aataaactct tactcttgaa attattttca tatatcaaat
    65161 aaataatggc tactaaagag gtaaccaaag agaaaagtcc caaattttta ctctcacgca
    65221 atttcttttt tgtgctttcg agtttccctg ttaatcgaag atttgcatct atcgtatctt
    65281 ctagattagg ttgaaataca atttctaatt ttttattttt cataattaaa tatacttgag
    65341 aatgttatgg tgttgatatc gtagaatttc atgtgtatat tttctaaatt caaaaaaaaa
    65401 tgtcaagagg agtatttcaa aatccattgc cgctcgttcg tggcagtggg tgaaagttag
    65461 tcttgatata cttgcgggta acgataggat tatctaatag aggttcagaa atcttaggtt
    65521 tatggttttg ctaccaaaga ataaaaactt gtttttactt agatcttttg ctaaatcagg
    65581 aaagaaaaat taagatatct gaattctttt gttatttccc ttctgccata aatatgagta
    65641 atgataaaat taactgttcc gacatcggct gaaaaaagta atgatcgttt ctccaagagg
    65701 cataagtata tccaaatggt acatacatat ctgctagaac tcccattcca tcgttaggtc
    65761 ctaatacagc catttcatca tagtagggca ttgctctttc ggacactgta cgtcttatag
    65821 gaattgccaa tacattgatt agttttattt gagtaggtaa atttaactcg atatcaagtg
    65881 gagttgaggg acctgtctga atagtcaata atcccttata atcataatta ttccacttag
    65941 catacattct ccaaaaaaaa ctagaatacc aatgttcttc cactgagttt gcaagtggcg
    66001 agccttttac taatccaact atattaaacc aaccaaccac tttttgaaaa tagggttcct
    66061 taccgcacaa ggaaatcgct atttttaaat cgctcgctcc cttggacaaa gttgtaaaaa
    66121 tcaccttttc ggctgtcgtt tgatttaaat aggtgcaaat aatatctgca ttctcgtata
    66181 cggaacccgt ttgtttgatt cgaatcattt tagttttata acctaaactt tctgccaatt
    66241 ttcgtaaggc aaatccctct gaatcaaact ctttattctt ttcataaaat aatccaggga
    66301 taaaaactag cgataaattc ttttgagaaa ggtcgggtat ttttttttct aaaagaggtt
    66361 tttgtttttc aatatactga tagaggttta ggtttttagg atctctcaat attctagagt
    66421 ataatagagc agctgcttca tcaatcccgt atttattgga aactaatctt aatttttcat
    66481 ttgttaaatc ttttacagaa ggataatctt ggtaattctc agcccaatct aaatatttta
    66541 attcatcgtt acatagatcc ttaataaaat atttattatg tgtgcaatct tttgaaaata
    66601 ccggttctgg tagttttata tttttaggtg ttgtggagca actgaaagat agaatgagag
    66661 aaaatagaat cgtagttatc gtaaaattgg aaggatagaa aaagtattta taagcattat
    66721 aagaactgat tctatttaaa aaagtttcta ttttatttat gatttgctgt aacaaaagga
    66781 attttttctg aatgagtgtg aattgatttg aatttgataa tagctgatag ataccagcta
    66841 ttatcaaata agggttagat tagtccccag aaactattgt acatttttgg agaaagagta
    66901 gacctaatac gttcaattgg ctgtaatcaa cgctaccaat tttagtaact tttccgtgtc
    66961 tagctgcagc tacaacgctc atatcgccat atgcaatcaa accataaaga aatatagatg
    67021 cacaagcttc tccggtttta gaaccaacac tttgtccgat acatgaatcg ttggagtttt
    67081 ggcaactaac gtttcctttt gaactaccaa aaagagaacc aggagccccg cccataccat
    67141 aagtaccagg tggatctaat cttgttggat agttatttgc acacgaaatc gccattgcca
    67201 atgcaataac gaaaactact aaaaaatttt ttttcatacg tttccctcaa ttaaacttag
    67261 ttttgccact ataagaaaga gtttgtaaaa atttaatttt ttttctagtg tcctcctttt
    67321 ttcgtgcacg attgataaaa aaatattctt tcatcaaaag tctgattgat aaacttgatt
    67381 ttcttgtatc cagtttagat aaaacatttc gtagggtcgg aacaaataac cccaaaaaac
    67441 atttcttcaa tttttgtcct aatgtatgag tgttttatca ccaatctaac aatgttttta
    67501 agcagtctaa atgaatcgat taagaggatg attattctta gtgataatgt tagattgagt
    67561 gcaaactata aacttttatt gtctataaac agaactgata attttgaata taatctgatt
    67621 tctctccgtg attcgccctt cggattttgt taattattga gtgaaaccca ttactgattc
    67681 acttttgttt taagcatatc ctaaaagaaa ttagatggta ttgtatttaa attgtaggag
    67741 ttgtggctaa gagttgttta cctgaaatgg tttgaatttt cagaaattat tgccaaatca
    67801 ttctctactc taatgcaaat tctataatgc ttgttgttcc gtttttactt gctacagagt
    67861 aagtgccttt caattgctct acaagcattt ttacaagcat tagtccaaat ccagtcgatt
    67921 tggtttgatg ttggtcattg ctgaatccaa taccgttatc ttgcatggtc aaggttatgc
    67981 ttttttcatt tttattaaca gaaataaaaa ttctaccatt gttcctttct ttaaaagcat
    68041 atttgaaaat gttagtcaat aattcattca taataattcc gattgtaata gcatgatttg
    68101 ctttaatatt aaattcagta atatccttct caatgacaat tgaatcttgg tcaacaaata
    68161 catcgtaaag ggaattgatt aaactatcga tgtaatcttt catagagatt tcatgtatat
    68221 cgtttgatag tagaagtttt tcataaagaa ttcgaatact ttggactcga gaaataacgt
    68281 cctgtaaagc aatttttact tcaaagcttt tagcagattg agcctgtaaa agaatgagac
    68341 cttcgatatt tgcaatatta ttttttatac gatgctgagt ttcttttaat aaaatttctt
    68401 tttcattcag aagttgattt aattttagat tattttcatg tagattatga gttttccaga
    68461 ttaactccaa atccttttca atcatatctc gaaaactaga aactagattt ataaataatt
    68521 cagaatattg attttcttta ttatcaagaa cacaaatagt tccaatggga gtcttatctg
    68581 gtaaatgaat tggaaatccc aaatatgaaa tcatattgag cttaacgtca ggattatctt
    68641 tccaatgttc atctgaaagt gcattgggaa caagaagcat ttttctagtt ttgattactg
    68701 tttcacaata caaacctgaa ttatcaaaat gctccttatc tcctggatga taaggattgt
    68761 gattcgtttt actagaaaga tatacttcta tatccgcgcc gattcttcgc ataattagac
    68821 cagtaggcac attaattata tttgccataa tggtaataat attttgccat ttatctttta
    68881 cttcatctgt tatctgagtg agttgtaaat tttctaattc ttccattgaa tcaatcctca
    68941 aacctatctt ggtttagaac catagtcaga ataaagggta tttacgagga tttcaactgt
    69001 tttgtgagaa tagattggtt tatttaatat tataatttgc atgttagttc attacagatt
    69061 gcattagtaa aaatgataca tctgaacgta aatgaataac tgagttttcg caaaaaagaa
    69121 aattataata tttaaagcag actgattcat aaattgtaat atagcttggc tgttagagag
    69181 cgagaaaaat ccagagattt ctgttgcctt gcttccggtg agttgatcaa gtctaaaaca
    69241 atcttcggta gagtttttta ttttctgcgg atactctgta ttagcctctt tttgagaagg
    69301 atccattcta acttctgctc tttgcactga tggttctata aacttcattt cgatttcaga
    69361 agcaatatca gtggaactaa ttatttttgc aggatgaatc tttagaaatc atattttttt
    69421 catattctct atttataaaa aaagtgcgta taataaaaaa tgaaaattat ttaacgattc
    69481 ctccctttaa aagtagcgac atacgttttg ctaatcggtt ttatttcttt attttaaaaa
    69541 gagattatac tttcctttaa actcagtttt gcagaaagca aaattcaata ttttattctg
    69601 aagggataag caatctattt tttttggttt attaaaatgg agaattacaa ctaaaaaaac
    69661 ttagagttta aaatgatccc agtaactgac cctctccgat actaaaaatt taaatctata
    69721 aatgaattct gtgttgagga ttgattctaa aataacattt aatactttaa agaatgttgg
    69781 gtcgaatgag tcttacgtaa aaattacccc attttattta tttaagatta agaaaactag
    69841 tatacaaatt tcatcttttc tttatatttg cgaagtttaa ctgatatgct aaagctttgt
    69901 tatttaatac ttttattttt ttattttcct ttgtttgctg attcggaaac tccaatcttt
    69961 gagttaaaaa gctctgaatt tactacagag ccgctgccaa agtatattta ttatttagaa
    70021 gatacgtcta gcgacctcga tatagaaaaa gttcaaacac ccgagataca agaaaagttc
    70081 caagcaccca aaccagatct tagctttggt tttaaaaaaa atccatattg gttcaagtgg
    70141 acagtaaaaa atcatgtaga tagaccaatc ccttggacat tggaaattgg ttatccaatg
    70201 ttagatcata ttgaattata tattccagaa gaggatggaa aatttactac aattgttgag
    70261 ggagatgaaa agccatttta cgaaagaaaa gtttattata gaaattttct atttgagctg
    70321 caagaaaagc caaattccga cagaacctat tatataaaaa ttatcacaac tagctcttta
    70381 agttttccta ttcaagcttg gactcctagg gcattaacag aaaaaatcaa tacggaacag
    70441 actcttttgg ggatgagtta tggaattatt tttatcatga tattctataa tctgtttata
    70501 cttctatcaa cacgcgatag gagctatatg tattatattt tatttgtgct tttctatgga
    70561 agttttctaa ctactttgaa cggtgttgcc tatgaatact tatggccaaa tcaagtatgg
    70621 tgggctaatc attgtttgcc tttctttatt aatggcggtt ccttgtttgg attgatgttt
    70681 ggtagaaatt tcttaaatct taaaaccagt catcctaaat acagtatatg gatacgtttt
    70741 ttaatgatcc ttgcatttat tggaggaatt ctatctcttc ttgctagtta ttcacttgct
    70801 attaaaatcg caacagctct cgcaggagtt acagtaggaa ccttattgat tgctggaatt
    70861 ttaggtgttc tgaaaaaata tcgtccagct agatactatc tgattgcttg gtttgctttg
    70921 attctgggaa ttgcttctta ttctctcaag gcatttggtt tactccctac aaattttttt
    70981 acacactggg gagttcagat cggggcggtt atggaaatgt ttttactctc tttggcgctg
    71041 ggtgacaaaa ttaatactct gaaagctgaa aaggagaaag cacaaaaaga attaaatatt
    71101 gcttatggaa gatttgttcc tcatgaattt ttaaattttt tagaaaagaa aagtatttta
    71161 gatgtgaatc tgggagatca ggttctcaaa agtatgacta ttcttttttg cgatattcgt
    71221 tcctttacat ccctttcaga gagtatgact cctgaggaaa attttaattt tataaatgat
    71281 tatctaggca gagtagttcc agtgtttaga aagaataatg ggatcgttga taaatttatt
    71341 ggagatggta tcatggctct ttttccaggg aggccagaag atgcagtttg cgccgcttta
    71401 gaatttttaa atatactgcg tgtttataat caagaaagaa tttctagtaa tcttacaccc
    71461 attcgagttg gtattggaat tcactgtggg ccattaatgc ttggaactat cggacatgaa
    71521 gaatttatgc aaggttctgt aatttctgat tctgtcaatt tagcttctag aatagaagag
    71581 cttactaaga aagtgggtgc tactgttctt atcagtgagg gagttttgaa tgaacttggc
    71641 gatagtagag aaaaatacaa tatacgatat attggaaata ttcgagtgaa aggaaaagta
    71701 aataatacca aagtatatga aatttttgat ggagactctg aagagattcg aaatttaaag
    71761 ctagttacta gagaagattt tggaaatggg gtagagtttt tctataaaaa agaataccaa
    71821 acagccaaag aattttttca gaaagtagtt cgtaaatttc cagaagacaa agcgactatc
    71881 acttatctgt ccagattgaa tgaagatacg ctttaatttt atttctagtg cctatcgcat
    71941 atatttcgga agttctggat cgagtgatgg agtgggaaca agtgcaacct tcaacaatcc
    72001 gtatggtgtg gcagtagata gcagtggggt agtttacgta gcggactcag ggaacaataa
    72061 gattagaaaa atctcgtgtt tgtagtcatg agctatttat ttttctaatc ttacaaaaga
    72121 tcgcctaact gttgtaagcc actattgaaa tattaaatct atctagatta tagattttta
    72181 cttttctaac tttttaaggt aagccaccac gtctttattt tttgagtcaa gcgcataatc
    72241 taaaggagat ttaccgaatt tattttttac ttttgtcttt gcacctttgg atactaaaaa
    72301 ttttaataca tctaattttt catacatggc agcgcgatga acgagtgtgt cttggtagag
    72361 gtttgcacta tttatatcag aacctttttc aacgagtaat tgaatgattt ttaaaggagc
    72421 agatggtatt gaaatataga ttgcattttc tccattgttt ttcttaggat tgacatctgc
    72481 atcttgcgag agtaagaact cagtgatttc gtgttttcta ttttcaagag ataggataag
    72541 aagtgttgca ccgttttcat cctttgtatt tatatcactt cctttcttta aatagttttt
    72601 tagcttttct aattttccta atttagcaaa ttctaatata gcattgttat cattctgtgt
    72661 tgattcagaa ttatttttgt ttgcatttag actcttgaga tattcaacca cttcctcttt
    72721 ttgtttttct agagccaagt cgagagcggt ttcttggtcg gaatttttta gagaaggatt
    72781 tgctttgttt tgaattaata cttttactac ttctagattc ccctggcttg cagcgatatg
    72841 caaaggtgtt tcttgtctat cattggttat atctacattg attccatttt cgataaagaa
    72901 ctgagtgaaa gctggatttt tattgaaact tgccagatga agtaaactat aattctcttt
    72961 tgctctatat cctaaatcta ctttgtgaac taatagactt ttaaaaatat ttacattgtc
    73021 tccccaaaaa gcagaacgaa tgattttttc tttttcattc tcagttaatt ctggatagta
    73081 gttcagtaaa ttttcgaaga gaattaaatt tccttgtcta gcagaatatt caagcggaga
    73141 tgttccagaa ttatcagttg tctttggatt tacaccttta tcaataaaaa gtttaagcaa
    73201 ttctaaattt tcggtatgag ctacatggtg tataacgtta aatcccctta ggtctacttc
    73261 atttatattt gccttgttct ctaagagtaa tttagctact tccaaatttc ctgtcatagc
    73321 ggagatgtga agaggtgtca tttctaaatc atttttacta tttacatcac aattctttgc
    73381 taaaagaagt tttaacattt caacatcgtt gacgctggcg cttatgtgta gaagtgagct
    73441 attgtctaac tctggatatt ttgtgttcgg attggctcct ttttgaatgg cattttctgc
    73501 atcggaagta tttccggttt ggacagcctt taataagtct tgattcggac ttttgcattg
    73561 taagattagg aaaatcagta gaatgctaaa aaatattttt gtcataaacc ctcttaattt
    73621 tatgaattta aagttgtaac tatttagatt ctgtgtaaat aaaaaaacat ttcaaaaaat
    73681 ccttttctat ttctctagcg gatatcgaca aatgcaaatt ggattctatc tgtttcctat
    73741 ttgtgttatt tgcggatact agagagtatc ctctttatat aatcgttaaa atatattttt
    73801 gtagtttcaa tttatttaat aaatcgaatg attttatcaa tactagattt ttattcatcg
    73861 aattcagaac tttcatatag atccataaat tcttcatttt cgcagtttaa acatttgcac
    73921 cataataaat aattatttcc aacttttact tctctgagtt tttgaatatt tgctgaatga
    73981 cattttgagc aatgtccagg atttgaaatt tctctatatg gaggatctgg tatcgtagga
    74041 tttataaaag tttcatactg atcgttattc aaaggattta tcttttcgat ctcaattatt
    74101 ttctcattat aggaatccca ttcttgtgat acttgtcgaa taaagtaggt attgcattgt
    74161 gggcactgat ataaatatgt aattccgctt tcacttttat ttattatttt gaatacactc
    74221 aaatcaaaat tagattctaa tagggaacca ctttcccaac cgttttgaag tatactacag
    74281 actggacagt tattctttat cataaatgtt cattgatcat atttctctat accaaacgtc
    74341 aaaagaaaaa tcccatatca aaactaaaaa ggcgggttag gtctaaacac ttcttagtat
    74401 tcccgaccca gatcattctg aaacagaaga cagatggatt gcgttttgaa aagttaaatt
    74461 ttttagaaat cattatagta gtatttgttg ataagtcttc tgatagggat tttatataaa
    74521 agacaaacga aaagtttagt atcccatata tctagatgcg gtggtcgaaa aacattttct
    74581 gagattgcca aagtcaaagg gaaagatatt agtattatca taaatcattt tctaaaaaaa
    74641 gaattagata ttcacaaaga cttgactatt taattttgat ttaatcgtct atcggttcga
    74701 ttcttgttat tcaaaaaaaa gcaaagattt actcgtgaaa gaatttaagc ctaagaaagt
    74761 ggtagtgtaa aagagaattc actgcctata cctaaattac tttctgcata aattttcccg
    74821 ccttgcattt ctacaaattc tttgcaaaga ataagtccta gacctgttcc tgtttcattg
    74881 tttgttccta aactactaaa ctttgaatct actgtaaaaa ttttggtaag gttttctttt
    74941 tcaattccaa cgccagtatc tttgatcgag atttctagga atgaatcttt ttgcttcgtt
    75001 gtgattgtaa ttttcccatt tgagtttgtg aacttgatcg catttgttaa aagatttcga
    75061 agaacagtgc tagtcagtgt ttcatcggcg aggacagctt catccttact tgcaattact
    75121 tctatagaaa tattcttatt aaatgcgttt cctttgatta acgcaacaga ttttgaaatg
    75181 agttggtgga tggaaagtgg ctcagggtgg tattcaattt gatctgtttg ggattttgcc
    75241 cattgcatta gattttctaa aagttcataa cctgattgag aagaagtgcg gataattctt
    75301 gtatactcca ttagagatga atcttgttgc ttattttgct ttagattgga ttcgattagc
    75361 tcagagagtc ttacaattcc gataaacgga ttaagtaaat cgtgagcaat gatactaaaa
    75421 aatttgtctt ttgttttatt gagagtttct aatttttgta aatttatctt ttgctcttgt
    75481 tcatttttct taatttcggt aatatccatc attatgctga gattgaaatt tggctttaag
    75541 cgtattgctt ggtattttac aattttagga attccatctt ttgaaacaca atcgaattcc
    75601 ccaatttctt ctccttttcg aatatattcc gcatatcgct ctatggcgct aggattggaa
    75661 attgtaacta gctcccccac attcatacgt aatagttctt ggatcgaata tccgagtagt
    75721 tcagaagcag cattatttac agcaatatag tttccgttat catctgctat taccatagca
    75781 gataaacttc tttcgaaaat gattttaaaa ttatcttcac tttctttgat ggaatcggtg
    75841 gtttgtcttt tattcgcctc atgaactgat tttagaaagt cattttcttc ttttaaagat
    75901 tgaatttctt ttatgaggtc ttcttttgat ttatttatat tgaacgccaa aattaatttc
    75961 ccatttttca atcacaaata agtgttcggt atatacaaac gctaatctaa taaaatccaa
    76021 gtagaaagtt tcttttgacg tttggtatat aaagtccaca tccctatcca aaaaagattt
    76081 taaaagggtt ttcttctata ctcaatagca attgcaattg ttgaaataaa tcatgttccg
    76141 tcaaggaaaa ttcttcataa aaacatatca tctctataac acctaacgca atccttaggt
    76201 atttagatag agcagttctc ttttgattta aaaatataaa aaactagaaa actttgagaa
    76261 actatcgtct cgaaaatggt gactcattac tcacaaaatg gatccggaaa tagaatctag
    76321 aaagtattca ttttttatga attctgtcta ctaggttatt tccaaaagaa tctatttttt
    76381 actgctaaac ttcttaccaa ctgtttcatt gttttcctta ctagtgaaag ctttgaatta
    76441 gaaatagatt gtaaatcttt taagaagaga tgtttaagcc agcagattga atgagtttac
    76501 ggtgcagtag ccgctacaga ggcagtataa gcaacagtta caaaaacact gtttccatat
    76561 acaacagaat accagttttg agaacttggt agagttcgag tcgtccatgt gattccatct
    76621 aaagaggtag cggcaactga atttggacct gtgggcactg ctacaaaagt gccattgcca
    76681 taagtaattg ctttccagtc tgaagagctt ggcaagctgc gagtcgtcca attgattcca
    76741 tcgggagaag tagcagcgat tgtagaagaa aaagccaatg caacaaagag tccatttcca
    76801 tatgtaatac aagtccagct ttgagaactt ggtagagttc tagccgtcca agtaatcccg
    76861 tcggatgagg aagcagctgc agtgctagat ccctgagaag caaccacgaa aagtccattc
    76921 ccataggtca cagtagacca gcttgctgcg ctgggaagat tccttgttgt ccaagtgatt
    76981 ccatctggtg aggatgctgc tcccgtcaca ttcatagcaa cggcaacaaa aactccattc
    77041 ccgtaagtta ctgaccacca ttgtgctgaa ctaggaagat ttcttgtcgt ccaattgatt
    77101 ccgtccgttg aggtagcggc tgtatttgta gcattagcga ctgcaacaaa tattccattt
    77161 ccaaatgtaa cagaagccca agacgaggct ggaagaagag ttatcgcagt ccatgtagaa
    77221 ccagaaggaa cacaattgac agcgactgaa acatcagcac tagcaatacc acttcctgca
    77281 gtcacagagc aaacgacagc cacaggttgc gttttaatcg tcactgcata cgctccggta
    77341 acctttgtaa caaaggtaaa cgacgttaat ccgctggcaa tcgtcaagtc atcgcctgca
    77401 ttgttttgta agactagacc agatgcagta agtcctgtaa ttgttccgcc tattgtataa
    77461 ctgttcgcgg ttgaagagga tggtgttgaa atttgatttg ttctatttct ttgtgaaaca
    77521 atagctgcta gagctgcggc agatttattt tcatctacat gtgatttttg acaatttaag
    77581 agaggcattg ttaatataat gacacttgct agtaaagtat tgaactgata aacccttcca
    77641 tttctaaaat caatcttcat cttcttcctc tcttggattc atttttatct gctaaaaatg
    77701 tttctacaga attagcatga tttagtgtat atatgcgata tacgatatat aacacataaa
    77761 ttattgattg ataagatcga aagcaagaat aaaaaaattc tttttcggtc agtttatttt
    77821 ttgacactca aaattaatgt ttaaggagag aaggatgttt aaataaaata ccactttgtt
    77881 tggaggaatg tattgagatt aagttttatt tttgttttat gaaattttca ttttgatata
    77941 agtatactat cgtatttatt ttctaatttt gagttttttt acgattattt actttctctt
    78001 caaagtgaaa tttttaaatc aaaaatggat ttttcaactc agatgattta caaataagat
    78061 tagagtcctc ccaaattatt aatcgagatt gtgtaatcgg gtggattgac tgcatagttt
    78121 gcatctgcgc tagttgctac tccaagaatg accgaatggt ttcgagtcga aggagtgctt
    78181 actccatcac cagtccatgt aaacgttaca gtttgcggta tattccaatt agccggagtg
    78241 aatagaagag ggtttgtaga tgtaatagta agtcccgtat ctggtaaagt cgcaacactt
    78301 actgacagag gaatactgac gttagccgta ggttggctat ttaaacgaac tgtaaaacta
    78361 tctcccagaa cagactctaa aactcctgaa ttgctaggat ttgtaagaat aaacccagtt
    78421 gtgtcgttat ctgaattcgt tgcatttaaa gaagggattg tagcggtaaa tgctgtgtct
    78481 ccgctcattg ctccaaagtt tatattgtaa gcgatcggtc catctgcaat tgaatcatca
    78541 actccagtaa ttgtaacaat ctgctgtgta ttccaattgg catttgtaaa actcagtgta
    78601 gcaggagata ctgttccttc tgctgtatta gaactagaca cagctattga aaccgtggtt
    78661 gtaggtctag cacgtaaatt gattgcaaag tatcgtttac cggcggcttc cgttgtagtg
    78721 taggttccgg ctaccgttcc acctgttgca ttgactgcag tgcaagttgc aactgtgtta
    78781 ccagtgcagg gaactaaatt atacaataac tcacgattcg tcgcattttg attgtctaca
    78841 taactattgt aaataccacc atttgcaaat ccacttgctg tttcggatga actcgcagtt
    78901 gctgtgattg tgtaatttgt atcagtcgct ggactagctg ctgttcctgt aactgtcaag
    78961 ggttgaggaa tattataatt tgccggcgta aaactgagtg ttgttgggga gaaacttgca
    79021 taaccacccg tagcggcaaa cgttagaacc acatttgaca tcggttgact cgttagactg
    79081 tattgcaagg atgcacttgc tcccgatctt gttgtgatta aatatccaga aacaagcgga
    79141 ttttgcgatt gttttaaaac tcctcttgta tcgttatccg taactatgat ggatggttgc
    79201 gtaaaatcag ttacagggac aagaccatta aaatatgaat caccacttgt agtcgaagta
    79261 attttcaaag gacaagatac gtctccatcg tccaccaaat catccacttt tgaaatcgta
    79321 atttgatttc ctgttgtaat ttgattccaa ttggcggaag tgatcgttaa agatgagggg
    79381 ctaatggtaa attgtgttgt tggagaacca acaggaagag tgcaaggata ggttgagtca
    79441 ataccaaaat taacagttac gtttgctgta ggtgcttgct tgagtaatac ccaaaataag
    79501 gcctgtgtgg ttccggcttc ggatatagtg gtagtatttc cagctaggac aattttcttt
    79561 ccagctccaa tatgataatt ggtgattgtc cttgttgccg ttactccatt gtagttgtta
    79621 taagttaaag cggtaaaacc tgaaggggga attgttccag tctctgctgc agatgcggtt
    79681 aatataaaat tggcgctacc tgttcctgct cctgttatac ctctcactcg aaacgtttga
    79741 tttgtagaga aattggcact cgtaaaagtg aaacttctag aatctaactg ggtgacgtta
    79801 tccgctgcta aaattttacc ttcctgtggt gcggcagttg gaacagaaac agttaccgtt
    79861 actgtatttc ctggaaatgg actcgagcct aaattcatat tgtattcggt ataccctccg
    79921 gcgctagagg ttacgttcga atagccggta gtcaaggtaa gattaatagc ttttgtatca
    79981 ttatcattga ctcttactgt tacatcagat ggatctaatc caccataatc acttgaacta
    80041 gttattggat ttaacacgag agtaaaatcc atagtattct catcataatt gtcatcgatt
    80101 ggagttactg taattgtttg agcagtattc catgtgcctg cattaaaggt aagactagta
    80161 gggctaattg tatattgcgt aggatcattg tttgtgagtg tgaaactaac ggcttgagaa
    80221 gtgcaattta caagtgccgc gggcgtgcta caaggatctg aatttaatct aatattaaat
    80281 gtttgtgctc cagcgccctc tgtaatcgga aacggattgg ctgctgaaat cgtaaatcca
    80341 attgtatcat tgtcaatatt ggttaatgta agttccccaa gaagattgtt atatgcggca
    80401 ttagaagatg taggaatctt cgttgcgtat tcagccgcac ccgttgcgcc tgggaaagta
    80461 attttaaatg ttaaattgcc atcgtcaaat ccatctgcta cactttgtat cgtcaccgtt
    80521 tgcggcatat tccaaccacc agtcgtagta tttgttcctg ttacagcagc attcgtagtt
    80581 ggagtaaaga ctaacgttcg atttgtaata gatcctccac ctccatttgg aattacaatt
    80641 ccttctgtta tatcagatga tacaatattg gaaatagtta catttgcggt aggaagagtt
    80701 gcaagaacta cttgagctgt tatagtagaa gttccatctt cattgaaact aacagcagaa
    80761 atagactgta gaagaatgtt cgggactcca ctgtcgacgt tagtcgctgg gacatcaggt
    80821 gtaggagtaa taacagagct cgcgtatttt gtatcggttg tagttcgtgc gccaaattga
    80881 actgcccgaa gttgtgttcc gtcagaagtt ccgtccacaa ctgctctaac tcgaatcgtt
    80941 tgcgggacat tccaaccgcc agtgcttgta tcattgatac catctatatt ggaatctgag
    81001 tttacaatcg cttgaccgga tgttggggtg aatactaacg ttctattggt tattgcagaa
    81061 tttcctccac ctagagtagg agcgataatg atttccaatg tatctgtgct aataataggt
    81121 ccaagagtca catttccagt tggttctgaa tttaaaacta cagtaatagt agtttcaaga
    81181 cccactcctc cagggtcatt ctcattaacg gtaactcctc ctacggggtt aatggtaaag
    81241 cctgagatat cgttatttat cacttgaata ggaatatcag ctggatcaag accagaataa
    81301 tcggtagtgc taatgattgg atctaataca agtgtaaaat taagatttgg attttcaaca
    81361 aaatcatcaa cagctgttac agttacagtt tgtccaacgt tccaattcaa ggatgtaaac
    81421 gtgagtgagc ttggggaaat ggtatactca cttgtattat tatttgtgag gttaatggta
    81481 atagaagctg gattacaact cacaggagtg cttggagtgt cacagggaga agaggttaaa
    81541 ctgacttgaa atgtttgtga tgatccccct tctgttacag aaataggact aatggaggta
    81601 gttggtgcaa gagcaacaat attaaatcct cttgtatcat catcttgatt tgtgaaagaa
    81661 ccagccgatg agagcgccaa attatcataa gccgcatcga gaggattaat cgcgactgca
    81721 tttggaaaga caaccgtata attaatactt ccatcattca taaagtcatc ttggcctgtt
    81781 aaggtcatta tctgcggaat attccagtta gccggtccaa aggataaatt ggagggggaa
    81841 atggttcctt ccgatgggtc gcttgtcgtt attcctgtta cagttatatt cccagatgga
    81901 attgtgttta atacgatgga aaattgagcc tgtccacctg cctctgtagt aattgcggaa
    81961 gtaattggga cgtaggtaaa gccagctgta tcattgtcta cattgactac agtgactaca
    82021 ggcattgttt tgtttacata aacttgatca ttacttgttg atgcactgct cgaaatcaga
    82081 caattttttg caccatcgag taggtaatcg tcaactccag taacagttac aacttgaggt
    82141 gtatcccaat ttttagaggt aaacgttaag gatccactta ccgcacattc ggacgttgta
    82201 tccgatgaaa aacttggaat cacaacatcc gctccaggtt gtgtggataa tactacatag
    82261 atacttgcag tctgtccgtt ttcattggtt gataaaccta acagaggtga tgctgcgata
    82321 ctcgccgttt ctttgtcggt gttaatagca aggacatccg ggattgtaag aactgaatag
    82381 ctataatcat ttgtttgaac agaattaaat cttactctat attgctgatt tccatctgca
    82441 ataccatcgg ctactccttg taaggtgatc gtttgcggct gatcaaaatt atccggtgta
    82501 aaagtaatac tggaagaagt gagtagaatt ccttctgccg gattagagga agtaatagaa
    82561 cctattacca catctgcttc tggtttttta cttagcttta atgtaaaaga tgcctgtcga
    82621 ccatactcat ttgtaaaaac agcatttgca ttttcaaata cataattttt tgcgagatct
    82681 cttgaaggag aaaatggtaa aggtatcata ccgaaaagtt taaatcctga tttggatgag
    82741 cattgaaaag taaaactaac tccaatacac agtatcaaaa ctttcctaat atttaaaaaa
    82801 ctaataatta gcttctttaa tttcatattt tccaattgag tattgtcgca ataaaaactc
    82861 agtaaataga tgggaaaagg atttctctca tttcaaactg tagacaattt cattgcagta
    82921 aatactactg attgtattcc ttaataaaat taatttacca aaccctaact ataaatcaaa
    82981 gaactaactg aggatgcaaa gaataatagt aagtttacaa tcttatagca ttgaatacta
    83041 caatgcttga attttcaact tctttttaca taacgtaaga ctaaggatag ttacaggaat
    83101 gatttccttt ttatcattcg aatgaaatta tgtcattcgt aagatttgat tttcccgtat
    83161 taacagccga gtcaacgcgt taggaatgga aaatgaacaa aattctactg gtttatagat
    83221 ttcatctcat ttgaaaaaat ctcttatctt cccaagtaaa ttggatttat ccatcatagg
    83281 aaattacaag cgtaattcta aacaaatgaa tataatatta gtttgcctca ctaccaaaga
    83341 attgactgtt tttattctca aaaagaacct tactgattca tttatggcta gttgcaaatc
    83401 ttctctggtc ggtgaattca gtcaaattcc ctcctttctt ttgtttagaa ataagaaagt
    83461 taggtgagag atctagtttt tttatttaca atagaaagaa aaaagtgaaa tcttttctag
    83521 gaaactataa cttatgaaaa acataatcat actattttta tttttatatc aatgtcacaa
    83581 aatacaaact caattcaatc cttcaactgt cgtattagtc tcagttggaa atgtgctctc
    83641 tgaagaaaaa aaaataaaag taggagataa agtatcattt ggtcaaaatt taaaggtagg
    83701 caagaattct ttttgtgatc tacaaatcat agatgctgag agcataatta cgattagatt
    83761 gcaaccagaa acggaatttg tcctaagaac taaaattact cccaatcaaa atatcatccg
    83821 tcctagaatt ataaaaggag aagctttttt taatattgta aaactttcca aaaaagaaga
    83881 attcactgtt gtggcaagtg caatcaaagc aaccgtcaaa gggactcagt tttacataga
    83941 agtagattcg aatggaaaaa caaaactagc ggtaaatgag ggttatgtcc tacttaaacc
    84001 aggagttatc gaacttgatg atttaccctt agaaaaaata aaacatttgg cgaatgctca
    84061 aagcataatt gaaagcgaaa accagaaaga gcttaacgtc cctacaggac atagcgttag
    84121 tgtatccaat gaaatggtta aaaaaacaaa tcaattcatc gaatcaattg cctctgctga
    84181 gaacccaaat tcttctattg caaatgaaat aagcagacaa aactatttca ccaaagagga
    84241 tattcaaaaa ctcaatcagg aagaagaaga ggaatacaag aaagtttttt tggagctacc
    84301 tccacttcca actgaatact catccaatcc agaaaagtta agattggctg tcctttctcg
    84361 cattcattca ttaagtgaaa ccatatcgat aagaatggaa tcgattttac aaagaagaag
    84421 agagactctc attacttatg atggaagaaa aattcatgga atagtttatt cggaaaaaga
    84481 tttttttaat gtaatcaccc taaacggcat cgaaagaatt ccagtaaaag aagcagaaag
    84541 tattatacta tactataagt gatagttgcc aacatattcc atagaaaata aatttataat
    84601 taaacacgta atctagtcta aattaagatt gaaatatata ggatactttc caagttattc
    84661 cttttcaatc ccttttttac agcatgaaat catttattgt aattctattt tctattctgt
    84721 ttttacagtg tgagataagt aaaaatccac tcgcacccaa tggacctttt gggattttta
    84781 tgttatatta taatctctcc aatatcaaag cttggaaata cacggacact gatctggttc
    84841 attataattt aaatgatccg atattgccta actctccagt cattccttcg tctgcagtct
    84901 taaatacatt taccatttca gaaccacttc catctgggct aaattttaat tcatccaatg
    84961 gaatcatttc tggaattcca acatctagtc tcgaagaaaa agaatttagg ttcactgcta
    85021 atttaaaacc gttacccgat agatcagaga atacaagcag tctgaaactc aatgtagatt
    85081 attttccaat gacaagtgga gtgaataatc ctttctcagc tgaatttcca tttccagatg
    85141 atttcaccta ttctaattat tcaatagagc caaatctacc gactggattt actattgatt
    85201 ctaaaacggg aatcatctct ggtccagtcc aaacagaaac aaaaaattta ggaacctaca
    85261 aaatcaaagc aactcgaaat gatggagtga acgtagttgg ctctgcttcc ttaaaagtca
    85321 ccgaatgggt tttggaggct tacctgaaag cgccgaatgc ggaggctggt gataattttg
    85381 gatattcagc ttctattgat ggggatacga ttgtagttgg agcgtatttt gaagattcaa
    85441 accaagtaac aatcacaaat ggaactcttg ctgtaaacgg aactggtgct accgattcgg
    85501 gtgccgccta tgtgtttaga agaactgatg gtatatgggc aaatgaagca tatctaaaag
    85561 ctccgaatgc tgaaaccagt gacaattttg gatattcagt ttctatttct gtagatacaa
    85621 ttgtagtcag tgcacatctg gaggatagca accaaacaac aatcaccaat ggaacattaa
    85681 ctgtaaatgg agctggtgct tcaaactcag gtgctgccta tgttttttat agaacaggga
    85741 acacttgggc gaatgaagct tatttgaaag cagcaaatgc aggagcaggt gatgaatttg
    85801 gtcagtcagt ttccattttg ggtgatacaa ttgtggttgg ttcaagaatg gaagcaagca
    85861 atcaaaccac tatcacaaat ggaactacag ctagtcctga taattcggca tctcaggctg
    85921 gtgctgccta tgtgtttcga agaacgggaa ccacttgggc aaatgaagct tacttaaaag
    85981 ctccaaatgc tggagtgggt gataattttg gatattcagt ttctattgat ggagatacaa
    86041 ttgtagttgg agcaagaaat gagtcaagta atcaaaccac aatcaccaat ggaactacag
    86101 ctagttcaga taattcggca cctcaggctg gtgctgctta tgtatttcga agaacaggaa
    86161 ccacatgggt aaatgaagcc tacttaaaag cacccaatgc agaaacaaat gatagatttg
    86221 gtctgtccgt tgctatttca ggaaatacaa ttgtggttgg agcgggcaat gaggattccc
    86281 ttcaaaccac gattacaaat ggaactttag ttcaagcctc cgatgtaagt gcaactgata
    86341 ctggtgcagc gtatgtgttt cgcagaacag gaaccctatg gtcaaatgag gcttacttga
    86401 aagcacctaa tgctgaatca actgatgttt ttgggtattc agtttctatt tctggagata
    86461 caatagtggt aggtgtacta ggggaggata gcaaccaaac tacaattaca aatggtcttg
    86521 cacaaattgg agtaaatggt gctattaatt ctggtgcagc aaacattttt aaaagaactg
    86581 gaacaacatg ggcaaatgaa gcatacctaa aagcgccgaa tgctgagggt agtccactaa
    86641 atggtgattt atttggaagt agtgtttcga tttcaagaga tacaattgtt gtaggtgcat
    86701 catacgaaga ctcactgcaa accacaatca ctaatggaac tttagttcat tcttcagatg
    86761 tgagtgcagt taattcgggt gcggcttatg tgtttaggcg gaaatgattt ataactagac
    86821 acctaatata gtctaactta aaattgaaat agcaaggata gtttcctaga tatttttata
    86881 tagcatgaaa tcatttattg taattctatt ttctattctg tttttacatt gtgagataag
    86941 taaaaatcca ctcgcgccga atggaccttt tgggcttttt actttctatt ataatctctc
    87001 caaaatagga gcttggagtt attctgaacc aaatcctctt tattcgaaag atcttcccat
    87061 tgatgcgaat gttcccaaaa catctcctct tgctatcgtg aaaagttatt caatgaaaac
    87121 gaatctgcca aatggtctta gcttaaatac aaccaatggt tacattacag gaactccttt
    87181 agaattggga agttctgagg tagaaatttt tgccaataat gatttcttta aagaacagta
    87241 tccgactaaa ctaaaaatta taattacagg tttttcctat tctcccacta attcgtgtgt
    87301 ttttggcagt gaagtcaata taacacccac attcagtgaa aattttaatg tgaacgatta
    87361 tcaattttct atttcaccaa atcttcccat aggggttact atcaatacta gcacaggtgt
    87421 tatttctgga agatgtttag aaaagagaaa ttcttcctat gtagttactg caaccaatca
    87481 attgaataca aaattcatcg ctaattttca actgaacgtt gtagcttggg aaaatgaagc
    87541 ctatctaaag gctcctaacg ctgatggtgt tccaggcaat ggggatgaat ttggaacttc
    87601 cgttgccatt agtggagata caattgtggt cggtgctgta aaagaagatt ccctgcaaac
    87661 tacaatcaca aatggaactt tagttcaagg ctccgatgta agtgcaactg atactggtgc
    87721 agcgtatgta tttcgcaaag taggagatgt ttggattaac gaagcctatc taaaagctcc
    87781 aaaccctgac ataagtgata attttggaaa ctcagtttct atttcaggtg acacaattgt
    87841 tgtaggagca tctaaagagg attcaagtga aactacaatc gtgaatggaa cgactgctag
    87901 tgctgacaat tcagaaggag attcaggtgc ggcttatgtg tttcggcgaa caggaaacac
    87961 ttggataaat gaagcttact taaaaggacc tagtaccgat gtaaatgatg gctttggtgg
    88021 atcagtttcg atttcaggag atacaatagt aatcagtgct ccgggagatg ataacagccc
    88081 ttcaggagtg gtcaatggaa cttatacatc taatagtggt ggtgttcagg attctggtgg
    88141 agcctatgtg tttcggagaa cgggaactac atggataaat gaagcctacc ttaaagctcc
    88201 taatcgattt aatgatgatg tttttggatt ttccgctgct attgatttaa atacaattgt
    88261 ggttggagct ttatgggagg attcaaatca aacaaccatt acaaatggaa caactgcaag
    88321 tgctgataac tctgctatga attcgggggc agtttatgta tataaaagga atggaactac
    88381 ttgggtaact gaagcttatc tcaaagctcc caatacagag gcaaatgatg aatttggtcg
    88441 ttccgtagct atttcaggag atacaattat agtaggagct actaaagaag attcactcca
    88501 aaccacgatt acaaatggaa ctttggatca agcctctgat gggggggcta ttgattctgg
    88561 tgcagtttat gtatttaaaa gaaatggaac cacttggact aacgaagctt acttaaaagc
    88621 acccaacgca gagggagggg atatctttgg aacttccgtt tctatttatg gagatacatt
    88681 agttgttggg gcaattagtg aagattctaa tcaaactacg attacgaatg gaactttgac
    88741 ttcatcgaat aattcagctt ccgcttctgg tgcagtctac atttttaaaa gaacaggaac
    88801 cacttggata aatgaagcct acctcaaaac accgaatatg gatgcgggtg atacatttgg
    88861 taaaagtgtt tctatttcgg aggaaatatg tgtagtgggt gcaaacaatg aagattcaaa
    88921 tcaatcgaca attaccaatg gacttgctac ggggggaaat aatccaaata caaatgcaag
    88981 ccaaggtgct gcttatgtat tccgaaggaa ataattttag aactggacac ctaatatagt
    89041 ctaatttaaa actagagaat aaaggatact ttcctagtta ttccttttta atcccttttt
    89101 tacagcatga aatcatttat tgtaattcta ttttctattc tatttttaca atgtgagata
    89161 agtaaaaatc cactcgcacc caatggacca tttgggattt ttacgttata ctataatctc
    89221 tctaatatca aagcttggaa atacacagac acggatctgg ttcattataa tttaaatgat
    89281 ccgatattac caaactctcc agtcattcct tcgtctgcag tcttaaatac gtttaccatt
    89341 tcagaaccac ttccatctgg tctaaatttt aatacatcca atggaatcat ttcaggaact
    89401 ccgctaacga gtttcgaaga aaaagaattt aggttcacag ccaatctaaa accgttaccc
    89461 gatagagctg aaaatacaag tagcttgaag atgaatatag attactttgc tatgagcagt
    89521 ggattgaata atccgttctc tgctgagttt ccttttccgg atgatttcac ctattctaat
    89581 tattccatag agccaaattt accgactgga tttttttttg attcgaaaac aggaattata
    89641 tctggtcccg tccaaactga aactaaaaat tttggaatct acaaagtaaa agcaactcga
    89701 aaagatggag tgaacgtagt tggttctgct tctttaaaag tcaccgaatg ggttttggag
    89761 gcgtatctaa aggcgccgaa tgcagaagct actgatttgt ttggaggagg gggtggtgta
    89821 tccattgaca gagatactat tgttgttggt gcaggtggtg acgattcact acaaaccaca
    89881 attacaaatg gaactttagt tcaagcatcc gatcaagtta gtgctattaa ttcaggtgct
    89941 gcttatgttt ttcgtagaac aggaaacact tgggtaaatg aagcttattt aaaagctccc
    90001 aatgcagaag gggcagataa ttttgcattt attgcgattt ctggaaatac aattgtagta
    90061 ggagctaatg gagaaggttc aaaccaaact acaattacaa atggcactct tactagttca
    90121 aacaatctag ctgtcaattc gggatctgtt tatgtatttc gaagaataga gggcatttgg
    90181 gtgaatgaag cttacttaaa agctcccaat acaaatacaa attatcaatt tggtcagtca
    90241 gtttctattt cgggtgatac aattgtggta gggtcaagaa tggaagcaag caaccaaact
    90301 acaatcacaa atggaactac agctagtcct gataattcgg cttctcaggc tggtgctgct
    90361 tacgtgtttc gaagaacggg agtcacttgg gtgaatgagg cttatttaaa agctccaaat
    90421 gctggagcgg gtgataattt tgctcttcca gtagcaattt ctggagatac aattgtagcc
    90481 ggagctaggc tagaagattc taaccaaacc acaattacaa atggtgcaac agccagtgct
    90541 aataacacag ctggagattc aggtgctgct tatgtgtttc gtagaacagg aaccttatgg
    90601 tcaaatgagg cttacttgaa agcgcccaat tcagaaacaa atgatagatt tggtcagtcc
    90661 gttgctattt ctggaaatac aatagtgatt actgctccta atgaagattc tctgcaaact
    90721 actattacaa atggaacgat agtgcaagca tccgatcagg caagcaatac ttctgtagga
    90781 gcagtgcatg tctttagaag aactggaacc acttggacaa atgaagccta ccttaaagca
    90841 ccaaatgctg agactggtga tggatttggt aattctgtcg ccatacataa aaatacaatt
    90901 gtagttggtg ctccagatga agattcactg caaactacaa tcacaaatgg aactttggta
    90961 caatcttcgg atacagctag tgatttaaga gtcggtgctg tctatgtttt taaaagaact
    91021 ggtaacacat ggataaatga ggcttattta aaagcaccta atgcagaagg tggcgcattg
    91081 ggaacaggtg ataatttcgg aggaagagtt tctatttcag gtgatacaat tgtagttgga
    91141 gctaattcag aggattctaa tcaaacaacg ataaccaatg gttctactgt tatagacaat
    91201 aatagtgcta ctgaatccgg tgctgtttat gtgtttaggc ggaaatgatt tatgatgaaa
    91261 acgttagtcg ccattttgat tgtagtattt ttatttcagt gtgaaatcaa aaaaaatcca
    91321 ctatcaccca atggaccttt tggagttttt actttttatt ataatctctc caatatcaaa
    91381 gcttggaaat acacagacac ggatctggtt cattataatt taaatgatcc gatattacca
    91441 aactctccag tcattccttc gtctgcagtc ttaaatacat ttaccatttc agaaccactt
    91501 ccatctggtc taaattttaa ttcatccaat ggaatcattt caggaactcc aacaactagt
    91561 cttgatgaaa aagaatttag gtttactgct aatttaaaac cgttacccga tagacctgat
    91621 aatacaagca gtctgaaact caatgtagat tattttgcta tgaccagtgc attgaataat
    91681 cctttctctg ctgagtttcc atttccagat gatttcacct attctaatta ttcaatagag
    91741 ccaagtttac cgactggatt ttcttttgat tcaaagactg gaatcatctc tggaccagtt
    91801 caaacagaaa ctaaaaattt tggaacctac aaagtgagag caactcgaaa tgatggagtg
    91861 aatgtagttg gttcagcttc tttaaaagtc accgaatggg ttttggaagc ttatctgaaa
    91921 gcgccgaatg cggaaggtgt gaatccagat ggggatagct ttggaattgt ttcaataaat
    91981 aaggatacca ttgttattgg tgcagctttt gaagattcac tacaaaccac gattaccaat
    92041 ggaactttgg ttcaggcctc agatgtaggt gcaacaaatt ctggtgcggc gtatgtattt
    92101 cgcagaacgg gaaccacttg ggtaaatgaa gcttacttaa aagcacctaa ctcagaggga
    92161 ggagatcagt ttaataatgt tagaatatca ggagatataa ttgtagtggg agctagtttt
    92221 gaagattcaa accaaacaac gattactaat ggaacattaa ctgtaaatgg aactggtgct
    92281 tcaaattcag gtgctgccta tatatttcgt agaacaagga acacttgggc gaatgaagct
    92341 tatttgaaag caccaaatgc tggagcaggt gatgaatttg gtcagtcagt ttccattgat
    92401 ggtgatacaa ttgtagtcgg agcaagaaat gagtcaagta atcaaaccac aatcaccaat
    92461 ggaactacgg ctagttccga taattcggca cctcaggccg gtgctgctta tgtttttcga
    92521 agaacaggaa acacatgggt taatgaagcc tacttaaaag cacccaatgc gggagcagga
    92581 gatgattttg gttttcccgt tgctatttcg ggagatacag ttgtagtcgg agcaagacta
    92641 gaagattcaa accaaactac aattaccaat ggaacaaccg caagtcctga taactcagct
    92701 gctgattcag gagcagctta tgttttccgc cgaacaggaa ctacttgggc aaatgaagct
    92761 tatttaaaag cacctaatgc tgaagcagga gatcgatttg gtcattcagt ttccatttcg
    92821 ggtaatacaa tcgtagttgg tgccttattt gaagattcca accaaactat tattacaaat
    92881 ggagtggctc caattggagt aaatggcgca acggactctg gtgcagcata tgtgtttcgc
    92941 agaacgggaa ccacttgggt aaatgaagcc tacttaaaag ctcccaatgc ggaagctaac
    93001 gatcaatttg gatggtctgt ttccatttcg ggtaatacaa ttctagttgg tgcaccagct
    93061 gaagattcac aacaaactac aatcacaaat ggaactttgg tgcaagcctc ggatacatct
    93121 agtgatttaa gagtcggtgc tgtctatgta tttaagagaa cggggaatac atgggtaaat
    93181 gaagcttatt taaaagcacc caatgcagaa ggtggcgcag gtggaactgg tgataatttt
    93241 ggttttcaat ttacaatttc aatttcagaa gatacaattg tggttggagc aagagacgaa
    93301 gattctaacc aaaccacaat cacaaacggt tcggttgtga ttgacaataa tggcgcttgg
    93361 ggttcgggtg ctgcttatgt attccgaagg aaataatttt agaactggac acctaatata
    93421 gtctaattta aaactagaga ataaaggata ctttcctagt tattcctttt taatcccttt
    93481 tttacagcat gaaatcattt attgtaattc tattttctat tctattttta caatgtgaga
    93541 taagtaaaaa tccactcgca cccaatggac catttgggat ttttacgtta tactataatc
    93601 tctctaatat caaagcttgg aaatacacag acacggatct ggttcattat aatttaaatg
    93661 atccgatatt accaaactct ccagtcattc cttcgtctgc agtcttaaat acatttacca
    93721 tttcagaacc acttccatct ggtctaaatt ttaattcatc caatggaatc atttcgggga
    93781 ctccggtaac gagtctcgaa gaaaaagaat ttaggtttac tgctaattta aaaccgttac
    93841 ccgatagacc tgaaaataca agcagtctga aactcaatgt agattatttc ccaatgacaa
    93901 gtggagtgaa taatcctctc tctgctgagt ttccctttcc ggatgatttc acctattcta
    93961 attattctat agagccaagt ttaccgactg gattttcttt tgattctaaa actggaatca
    94021 tctccggacc cgttcaaact gaaactaaaa attttggaac ctacaaagtc aaagcaactc
    94081 gaaatgatgg agtgaatgta gttggctctg cttccttaaa agtcaccgaa tgggttttgg
    94141 aagcttacct gaaagcgccg aatgcggaag gtgtgaatcc agatggggat ttttttggag
    94201 atgtttccat agataaggat acaatcgtgg ttagttctag aggtgaagat tcgctacaaa
    94261 ccacgattac caatggaact ttggttcaaa cctcagatgt tggcgcaacg aattctggtg
    94321 ctgcctatgt gtttcgtaga actggctcat cttgggcgaa tgaagcttac ctcaaagcac
    94381 cgaatgccga attgggagat aattttagag ctattgcgat ttcaggagat acaattgtag
    94441 cgggtgcaac tggtgaagat tctctccaag caacgattac aaatggaact ttggttcaag
    94501 tcgcagacca agcaagtaat acaagtgtag gtgcggctta tgtgtttcgc agaacgggga
    94561 gcacttgggc gtttgaagca tatctgaaag cgcctaacgc tgagggtgtg gcaccaaatg
    94621 gcgataattt tggagactca gtttccattt caggagatac aattgtagtt ggtgcaagct
    94681 tagaagactc cctccaaaca acgattacta acggaacttt ggtgcaagcc tcagatgtta
    94741 gtgggactaa ttctggagca gcctatgtat ttcgcagaac gggaactact tgggtaaatg
    94801 aagcatatct gaaagcacca aatgcaggag gaggcgataa ttttggagga ttgtttaaca
    94861 tttccatttc gggtgataca atagtaatcg gagcaagatt agaagattca aaccaaacta
    94921 caattacgaa tggttccgat gttatagata acaatagtgc ctcaaattct ggtgcagcgt
    94981 atgtgtttcg tagaacggga agcacttggg caaatgaggc ctatctaaaa gcccccaatg
    95041 cggaaacact tgatcaattt ggaagatcag tttccatttt taaaaataca atcgctattg
    95101 gttcgatttt ggaagattca ctccaaacaa cgattactaa tgggactttg gtgcaaacct
    95161 cagatgttgg tgctactgat tcaggggcag cctatgtgtt tcgtagaacg gggacgactt
    95221 ggacaaatga agcatactta aaggctccaa atacgatagg tgataatcaa tttggctggg
    95281 ttggt
//
LOCUS       DAOQOS010000003        93769 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_70_length_93769_cov_6.964338, whole genome shotgun sequence.
ACCESSION   DAOQOS010000003 DAOQOS010000000
VERSION     DAOQOS010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 93769)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 93769)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..93769
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70_length_93769_cov_6.964338"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(96..1520)
                     /locus_tag="PLF66_01020"
     CDS             complement(96..1520)
                     /locus_tag="PLF66_01020"
                     /inference="COORDINATES: protein motif:HMM:NF016218.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF459 domain-containing protein"
                     /protein_id="HMZ62641.1"
                     /translation="MNLSKTLPVLLIAIVFFSNCITRQVPIPKERLIVKSFSINSKHH
                     KEIPYLIYLPNSYYTNSKEPMPLVLFLHGRGERGNDLNLLKRQALPKYIAEENADYPF
                     IMIAPQLSESEEEWYTQDLIKLIEDIESDYNIDEDRIYLSGISLGGNGVWKLATEYPN
                     KFAALIPISGWGDTSRICKIRNTNVWAFHGARDTLITPDKTIDVIDRLQYCNQNTWYT
                     IFPNAKHDAWTEAYKNSILIEWMLEQRKLKPKADQVKAKKGKITRSNPLKILVIGDSI
                     GISISWGLNEYVQANYHIVLDNFAKVSTGLSYPKFYNWPERLEEFLKQKKYDLGIVLI
                     GANDPQMMISDLGNVSFRTDEWKKEYLERISTIVNKFYAAKMNLYWIELPPMGPSKFH
                     SETRFINSCFKEASERYNFTYISMTNVLGNENGEYEKFLPYRGKRTLMRADDDIHLTV
                     VAAKKMVREILIKIFSEYEFPDNE"
     gene            complement(1815..3251)
                     /locus_tag="PLF66_01025"
     CDS             complement(1815..3251)
                     /locus_tag="PLF66_01025"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004441325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HMZ62642.1"
                     /translation="MLFNSVNFLIFFILFYIFYKRSNLKTQNWLLLLGGIIFYSFWNY
                     KMTFLLLFCIYFNYWAGILLDKSSDLPKRKKILTFTILLNLFILFIFKYTLFMIQSIN
                     DVFAFSGGKFKLSAIEIILPIGISFYTFHNISYVYDVYKYKIHATKNLLTYGVYDLFF
                     PLLLSGPIERAERLIPQIENERQITKEKFQSGLILVIWGIFKKVFISDNLAPFVNLAL
                     DPNENIPTGIVYFVAAAFAFQVYADFSGYTDAARGMARMIGFELSLNFNLPLFKATNP
                     SEFWKRWHISLSTWLRDYVYIPLGGNRFGLFRQNLNLMIVWILGGLWHGATYGYLIWG
                     IYCGLQVVFYNLFAEMKNKSNLLKNSKVEVFFLNPFIKISFIFITFLMFGLGLLLFRV
                     ENPSHLMRLIDNASGIYINSVFLLKFLFFISPIIVLDGLQVWKKDLEYFSSKNINPIF
                     AYTLLIIFSIQFCLFSVFESKEFFYFQF"
     gene            complement(3267..5444)
                     /locus_tag="PLF66_01030"
     CDS             complement(3267..5444)
                     /locus_tag="PLF66_01030"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HMZ62643.1"
                     /translation="MSFLQKALKLLQDFPNFRNQNFQVEGKKKKSFLKEAENFFKNLN
                     STKDSANDLDEEKPLSDNKDTLVPEDLDSNPDSLSFNEGEVVSAPVIPTEFNEEDNIV
                     VDLDQEEIIVQENNITSNENVEVNSIDDYTNKVESLDEFSGDTQTLELDEDTVIVTDE
                     EQSQELESNDILEDWERDAQEEALPEIEAVNEETKPTEEQPNPSFDSEIVEIEPNAPI
                     DSETIVDVNVEDAFDSAKQFKTSYEEKLESYLVLFEITKELLKSKSFDDFFENLMYSI
                     ISQIAPEIMIIFSSRDGNFSQMKIVAHEGIDIEPDYVIKKGDTLYTLLESETNVLYAR
                     DLLDKNLKDRERVLLENPLTELLIPIRVLDQFSGFIVAGKMISGEDYSEKDLEFLRIL
                     VEVSGNFLSKLFDSENLANEVSELNKIINSSSLITDFTDKVYTTKTLDELYDVISENL
                     VSDFKVSNFTFMILDVKTMDYKVFGSNFLLPETVDRFKLSKDSKIITMIAQVSDVYRL
                     ENFQDNFELKSLITEQEIAAMTEFTVIPLINIGRMVGLFIIHKVLTDWSPGFKKTILS
                     LSNIVAPIIANLVMQNEKESLFRNPFNPILETIDREIKYSESKNKQFTLVVLKILNVT
                     RILNILGINFFSDYSDFISKKIFENIKSSDYVSRIGQGKFAILLKENSKSDSEKFIEK
                     IKSEIVLFHNPSRDFKLSIQIYSLTFPDQARERRKFVEMMEET"
     gene            complement(5447..7765)
                     /locus_tag="PLF66_01035"
     CDS             complement(5447..7765)
                     /locus_tag="PLF66_01035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62644.1"
                     /translation="MAEESKQSELANDDLKTDRSVGSVVDERFADLEESLDFDVYVDD
                     IAYMTDEENEGTNVISSGYTSHFTDPSSDSPKPIPFNLSSLKEVEADELGSKPFSEDE
                     LDDILLRDTSDDTDIALSESELNNIVGGSEDSHEGDQFEDRYDTFIEEDDEDNITLAT
                     DELDQILNPDEDEDSEENEFVTKSFFDTEDSANEDDDIALSPDELNNITSLEETDSTL
                     DETANIISDPPSDLFEYSENEDDIALSGDELNNILESGEVSSEDEISDESEIKSFFDT
                     DESGESEEEIALSPEELSRITDSEYSEEDGIETEDFDPDQQEQNISFDTPDEEESIAL
                     SGDELNNILESGEASVEEEEEDFDIDNAKSFFDTDDGEESIEEDESISLSPDELGNIT
                     AEENFEVEDAESPNDSVNTDDNFDLSEVSDELNIDETSSDLFESSNEDEGEISLSGDE
                     LNNILESGEIESEEVDTSGNSFFEESNEAEDDSISLSPDELGNITADENYEVEEIDNS
                     NEISNESDDTVELTDASDEFAIEESQSDLFESSNEDEGEISLSGDELNNILESGEIES
                     EEVDTSGNSLFEESNEEDDSISLSADELGNITADENYEVEEVDNSDLGTTESEDNFSL
                     SDVPEDLDVESPSSFFEEESVADDDIALSGDELNNILETGNLETEEADYSNPIDSGMV
                     INDDDFVIDENSSLSDLGDVTIDETGLDHDAEATDSSESFHFGNVRKEELRKVISYMD
                     ELLGSLPDSYIKDFASSEYFELYKKIMNELEL"
     gene            complement(7776..7997)
                     /locus_tag="PLF66_01040"
     CDS             complement(7776..7997)
                     /locus_tag="PLF66_01040"
                     /inference="COORDINATES: protein motif:HMM:NF022007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HMZ62645.1"
                     /translation="MRQNRVWLKGIYAPTKIHVKIIGKVQNPGIYEMIQGSQLKDLVD
                     TAGGFTNNVDNSNLNLDTEVKDGQVIRIE"
     gene            complement(8220..8600)
                     /gene="atpC"
                     /locus_tag="PLF66_01045"
     CDS             complement(8220..8600)
                     /gene="atpC"
                     /locus_tag="PLF66_01045"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HMZ62646.1"
                     /translation="MSDSKLTLSVISPEKEIFSGKADYVNLPGAMGYFGVLINHTPIV
                     SELEYGILEVKLDGKIQKIVVDGGFAEVKSNQIKVLTNGGDLKENIDPVKSEADLEKA
                     IESSSKTRDLDIKKAKSRVLIHKS"
     gene            complement(8602..10011)
                     /gene="atpD"
                     /locus_tag="PLF66_01050"
     CDS             complement(8602..10011)
                     /gene="atpD"
                     /locus_tag="PLF66_01050"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020045332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HMZ62647.1"
                     /translation="MSANVGKIKQVIGSVLDISFENGQLPEIFNALEIDVEKNGAKER
                     VIAEVQQHLGDNTVRAIALSSTDGMVRGLAVKDTGASISVPVGEVTLGRIFNVLGETI
                     DEGAPITAKEKRSIHMASPKYEDLKPKTEVFETGIKVIDLLAPYIKGGKTGLFGGAGV
                     GKTVLIQELINNIAKQHGGYSVFAGVGERTREGNDLWREMKESGVIDKTVLVYGQMNE
                     PPGARLRVALSALTMAEHFRDSANADILLFVDNIFRFSQAGSEVSALLGRMPSAVGYQ
                     PTLSTEMGGLQERITSTQHGSITSVQAIYVPADDLTDPAPATAFTHLDATTVLSRNIA
                     SKGIYPAVDPLDSTSRVLNAEVLGEDHYTVAREVQRILQRYKDLQDIIAILGMDELSE
                     DDKILVARARKIEKFLSQPFFVAEAFTGSPGRYVKLADTVRSFKELISGKYDDLPEQA
                     FYMVGSIDEVVEKAKKLKA"
     gene            complement(10037..10900)
                     /gene="atpG"
                     /locus_tag="PLF66_01055"
     CDS             complement(10037..10900)
                     /gene="atpG"
                     /locus_tag="PLF66_01055"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000235141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HMZ62648.1"
                     /translation="MASPREIKKRIVSVSNTKKITRTMEMVATAKSKKMSNRVNASKP
                     FSDKIKELVGSLSSLASKVESPLLRREENPKKIAILVVTANRGLCGGYNGNILRMTRS
                     FVAELKSKGIAYDLYVIGKKGISYFRFAKEPIKQSFINIDDNSGYKEAEFFANLFMEE
                     FSNKRIDGLEVISTVYHSSADQRSEITRVLPIEPPSDAQEVSESIIYEPSPESILNSL
                     LPLVIKTVLYKIILEAVCSEQIARRIAMKSATDAASEMIKVLVRGYNRVRQSKITQEI
                     SEIVAGADSLK"
     gene            complement(10913..12421)
                     /gene="atpA"
                     /locus_tag="PLF66_01060"
     CDS             complement(10913..12421)
                     /gene="atpA"
                     /locus_tag="PLF66_01060"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HMZ62649.1"
                     /translation="MKIKTEEITSLIKKEIQSYKQDLAIDEVGTVIEIGDGIARVFGL
                     KNVMAGELLEFQNGVRGQAFNLEENSVGVVILGEYKHIEEGFTVKRTGSIFSVPVGEG
                     LLGRVVNPLGEPIDGKGPIHTDKVRPVEILAPGISKRYPVSEPLQTGVKAIDSMIPIG
                     RGQRELIIGDRGTGKTTIAIDTIINQKGSGVICVYVAIGQKASTVAGVVQKLQDKGAM
                     AYTIVVAAGASDPAPLQFIAPYAGCTMAEYFMYDQGKACLIVYDDLTKQAVAYRQMSL
                     LLRRPPGREAYPGDVFYLHSRLLERAAKLDQKYGGGSLTALPVIETQEGEVSAYIPTN
                     VISITDGQIYLQSNLFASGIRPAVDVGISVSRVGGAAQIKAMKTVAGTLKLDLAQFRE
                     LEAFASLGTELDATTQAQLDRGNRIVEVLKQPQSNPYPVEDQVLVIFAVTRGLMDTIP
                     VAKVKAFETYLLNHVKTHHPELLNEIRDEKKIKDENVLSERIKEVVADFLKK"
     gene            complement(12411..12977)
                     /gene="atpH"
                     /locus_tag="PLF66_01065"
     CDS             complement(12411..12977)
                     /gene="atpH"
                     /locus_tag="PLF66_01065"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HMZ62650.1"
                     /translation="MDNIHVGKVYAEALLEIAAEKKNSVAIEEELQAAVKAICDDKQV
                     WEFFLSPKISKEQKIKVIESSFKNSLSETVNSLLYLLLRKDRIFFIREIAHQFSLGND
                     RLNGRIRAYVESASKLSDSQVSEIRSALSQKYNGECIIENVIKPELIGGMVIRFNDNL
                     IDGSMKSQLTNIKKNLLQSKLGGAYYEN"
     gene            complement(12981..13496)
                     /locus_tag="PLF66_01070"
     CDS             complement(12981..13496)
                     /locus_tag="PLF66_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HMZ62651.1"
                     /translation="MIYLAAGGLLDANPGLLVWTIVTFLVVVFILRKFAWNPILHALD
                     ERAEKIHGDIDKADKIRKEAEELLATYKAKLDGARDEALAIVNEANADAVKLKSKTVQ
                     ETQDEIKALKEQSLKDIELAKAKAVQELQAQVVDLSVMIAGQILQKKVRSEDHAEFVK
                     EEIAKLKNLKV"
     gene            complement(13513..13800)
                     /gene="atpE"
                     /locus_tag="PLF66_01075"
     CDS             complement(13513..13800)
                     /gene="atpE"
                     /locus_tag="PLF66_01075"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010412179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HMZ62652.1"
                     /translation="MDFGLGYIGVGIGAGLAILGAGLGIGRIGGQAVEGMSRQPEAAG
                     KIQTAMLISAAFIEGAALFALVIAFQAGGVLNKELPNSLKAHTPAAVEQPK"
     gene            complement(13843..14856)
                     /gene="atpB"
                     /locus_tag="PLF66_01080"
     CDS             complement(13843..14856)
                     /gene="atpB"
                     /locus_tag="PLF66_01080"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002117240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HMZ62653.1"
                     /translation="MINKVIFILLSLFLTFSSFASEGHEPEAFDLPSIIDHHLFDHAE
                     FPLNFGGVKVYEGDNLFDKNNPAIFEEHSDGSHGKKYHFVGGFDMHITRRVTMMWIVS
                     FFLLAVFIPVARVIARNPLRIESRMAGAIETVLEFLRKEVIDPNMHGHGHSYYHYLFS
                     LFFFILFCNLFGLIPSVGEILAYATGQDPHHSILTKIWSGITVTGDVSVTVTLAGITL
                     LLIYGTGFSYQGIAFIPNSVPNGVPWPLWPLLWPLEFIISPIAKAFALTVRLLANMTA
                     GHVVILALLGFIFEFRSYAIVPISIGGATAIYLLEIFVAFVQAFIFTLLTSLFVGSSM
                     HRH"
     gene            complement(14849..15247)
                     /locus_tag="PLF66_01085"
     CDS             complement(14849..15247)
                     /locus_tag="PLF66_01085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62654.1"
                     /translation="MMQAFDLSKVDKIKFNLSYLSLSAIYLFISSRIEARYFSNPDLV
                     FTLGVFIGLLIQILLLNLRIVIMQKNLKLAGNLQIFISFFSILINVIILFILVTYLNN
                     FSLVFGFFIAHNLNILNIALITNRSTKKDD"
     gene            complement(15804..16187)
                     /locus_tag="PLF66_01090"
     CDS             complement(15804..16187)
                     /locus_tag="PLF66_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015675792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family membrane protein"
                     /protein_id="HMZ62655.1"
                     /translation="MKIFLLIIRILMGALFLFSSVTFLFKLIVPPEMTGAMKVFNDGL
                     NASVYLMPTVKVVELICGLALLSNRFVPLALVLISPIIVNIFFVHIFLAPEGLPVAIF
                     LIIANSLLAYQYKEIYSPLFKSKVD"
     gene            complement(16184..16666)
                     /locus_tag="PLF66_01095"
     CDS             complement(16184..16666)
                     /locus_tag="PLF66_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016762299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HMZ62656.1"
                     /translation="MSSLDERELVLERIVLAPAEVIWKCWTNSEHLKPWFCPKPWLVT
                     DCRIDLRAGGEFYTLMKSPDGEEHPNHGICLEIIPNQKFVFTDAYTAGWIPSKNPFFT
                     GIIELESLSNGKTKYTARARHWTLENCKQHSEMGFYSGWGIALDQLLDYIKINEGKSL
                     "
     gene            17104..17565
                     /locus_tag="PLF66_01100"
     CDS             17104..17565
                     /locus_tag="PLF66_01100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62657.1"
                     /translation="MESNQEFIYIIFIFLICIASFSGLWLTILFLTSYFGTWSKLYNR
                     YNFPKTLSQPLLIKKFQSAQIGISNYNAVLTLTFYKEGISFEPIFLFKFAHPKFLIPW
                     KDIKLNKRSQSIFSWNKLDIGNPPFAKINVSDNLLETILAYSEKEETDDRW"
     gene            complement(17567..18235)
                     /locus_tag="PLF66_01105"
     CDS             complement(17567..18235)
                     /locus_tag="PLF66_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010573619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HMZ62658.1"
                     /translation="MKEKKYLKEIEKGNFALALTMIDKELIERPEDPELLYNFAICCS
                     RTGNHKKCITILKTLLDKVGKFIERDNVFRLIIFSLIQLQEYKEALDLTDERLRVNIG
                     DLKLLSFRAHIMEKLNRLDEAIQIHRNILKLNPEYTNSLNSAGYLIATKKNPTAEEIA
                     EATDYLKQAVKQNPNNAAYLDSFGVLLHKRGDKQAAIKAFNKAIAANPALSGEILDHL
                     QTLI"
     gene            18393..19331
                     /locus_tag="PLF66_01110"
     CDS             18393..19331
                     /locus_tag="PLF66_01110"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HMZ62659.1"
                     /translation="MNILITGGSGVIGTRFISKVLHKHKLIAIGKNFSKFPEFIRLHK
                     NFKFYEYDLTSEKKFNIPDKINCILHLAGAVSGSTHNTEDYMRTNVLGTNKLIIFAKE
                     NKIPNFILASSVSVYGHQPGKKLVETDLLAGNTDYAISKIDAEKLVQMSGLENYTIFR
                     IASVFGKDTKGFITKLERIIKKRILPFPEDRNSKKSFIHILDLVEFLERAIENPKKGI
                     FNLAHSDSFSFSEISVILKAKTKSNFVIKVFIGKILLSFIHKTNQFLYFLKITKNPKR
                     IDLRPLLEFIEVNPKKAIQQFAYLPKIHLNSDWEHL"
     gene            19328..20743
                     /locus_tag="PLF66_01115"
     CDS             19328..20743
                     /locus_tag="PLF66_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008593996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HMZ62660.1"
                     /translation="MNSTLEHKNKIISILGKEKVFFREDGEMDAGTFDSYGTDRTKVH
                     KPNYDLLTFPKTTEEVAALVKYAYENNIKIVPSGGRTGYAGGAIAKDKELVISLSKMD
                     SVIDFDPFFASLTVGGGMITQNVHKEAEERGYIFPVDFAATGSSHIAGNIATNAGGVR
                     VVHYGLIRNWVLGMTVVTGKGEVLKFNGSILKNNTGYDLKHLFIGSEGTLGIITEVTL
                     KLTKKSIDTSVILLAVPDYQSILNVFKETHSSVLPLLAFEFFSDLCCEKVKHHLSLGD
                     PFTEKSPYYVLLEFEIAEESDNDKLYSFLEQITEKGLINDGSIAQNSRQKQTFWKYRE
                     GISESLSLQHTVHKNDISLPLRNMEQFISDMESLLATKYPGFEIALFGHIGDGNLHLN
                     ILKPANLSDSDFFAACKKVDPDMFSLIQKHNGSISAEHGIGLLKKDFLSFSRSAEEIR
                     LMKEIKNVFDPKGIMNPGKVL"
     gene            20766..21527
                     /locus_tag="PLF66_01120"
     CDS             20766..21527
                     /locus_tag="PLF66_01120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62661.1"
                     /translation="MKYFLFFLLITLSVIGQEKPTDQELIEKLDRTFLIKEGLTKGRL
                     YIKKGNHESHSWNVSIFKEGEDILYIFTVTHFKPVAKLLSIKRGQKLIYSNVLSGKIF
                     QIEQLQRLENILGTSFSYIDLSNYQYESNYKPFKPENSEKENQEKDRLSIHMIPISHY
                     LYKDIEIDMANTDYSLRKLTFTSPNGIIEKTLKVKYGKIKQIDGKAKSEIIDLKFIEI
                     TNNATSEISILEFQEWYKDTKPDKIFYEMKSLYEK"
     gene            complement(21590..22351)
                     /locus_tag="PLF66_01125"
     CDS             complement(21590..22351)
                     /locus_tag="PLF66_01125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62662.1"
                     /translation="MLSRYNIHADVKDFFDRKNQLVTEIENYERKIYSEKDDLDKIDK
                     LFKQLQVLVEKTNSEYFRLRLVTLDKLLKRVHEKRTYRKIEFDRIFKTLEKIKEDDSI
                     EFLNEGIRNRIEKISERIVRSSQKLDIEFGERFFLLTYKSLSFLVKEYEKKVLQDVDA
                     TKTFIRIKNKRYPLFPAYTGLKTDGKETKLDYCNILILKTKNDYKCFRFDQMEIITDL
                     TYDFLQAKLIELPEPVQEIKQYIRLRGKRVYYLDI"
     gene            complement(22364..23881)
                     /gene="rodA"
                     /locus_tag="PLF66_01130"
     CDS             complement(22364..23881)
                     /gene="rodA"
                     /locus_tag="PLF66_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016757024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HMZ62663.1"
                     /translation="MMAERRLEKIDYFLIISVVVVVLCGIITLYFQESNITDGPGKWY
                     KQLFYFIVGLTTMYFMSRLNYQLLGSYALPIYLFSIFLLILTLIPGIGYLPNGRGARS
                     WLKLGPFSFQTSEFAKLSTVILLGQYLVLREREIRNITVLVIPFIIVIIPMVFIIVQP
                     DFGTAVSFIPILLAMLFLGGADLMHISSLVILGGISLSIPMYLEYTRLTLMAPLVELL
                     TKTEKTEVLSIVNQLGGKIWPVLNGETIQAKEVSGLINPKNFKILQDAVDQVIEENTS
                     FLFKIFSNENFMIMFGGTLIFISLVLIALRITRGSENLRKYYIPLGVLGISFLFAFAV
                     SKKIPFRENQVIRLTAFINPDQFKQGAGYQLRASKPAIGSGRFVGKGFGHGEMTEGRI
                     PHVPESSTDFIFASWAEQTGFIGSVLLLFFLLAIPLRGLQISFESKDRFGSLLAAGIV
                     ALIFFHMAINIGIVIGLMPITGLPLSFMSYGGSHLLMSMVAVGIILSIKLRKHAN"
     gene            complement(23878..25827)
                     /gene="mrdA"
                     /locus_tag="PLF66_01135"
     CDS             complement(23878..25827)
                     /gene="mrdA"
                     /locus_tag="PLF66_01135"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015676872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HMZ62664.1"
                     /translation="MARNTTSSTEFKLEQSFRYRLYFFTGLIIFTLIAFILQLFNLQI
                     INGSENSLKAERFVRRSESLPAARGQVYDRNFINPEMSTQHVLISNSASLDVVVNTGL
                     LRNNPERIKEFMLLFYKTLSIPEAYYADEIKEPKFTKKVRSKIPIVLLQGISSEQHER
                     ISVFDNIAKYVILVPSPKRNYIMGPALAHVSGYVGLPNLKDLTNREIKSYQLVGKDGL
                     EIQYDSYLRGTDGFRYQKRNSEGNIEEERIIEHAKMGNHLVLTIDKKIQFAAYNALKN
                     YRGTVIAIKPATGEILALTSNPSFDPNLLSGKNKQLKLQHLNRVKNNGGFLNIALRSK
                     FPPASTFKTLVGLAALESEHKIDFSPSQTFHCNGSFILESTLAGVPDQEFKCWDKKGH
                     GTNDLMHAIEKSCSVYFYNLGHKLGSEAILYYAKLFGLDKKSNIDLPGEIEGFVPSNE
                     WKKRAYGTKWFAGDTVNLSIGQGFISTTPMGMTLFYMALLNNGKIFQPYVVSEIRNPI
                     DNSIVFKNPGKMLRDIPLKSSTLEALKQGLRAVVKTGTASRVLNLPDLPEIAGKTGTA
                     QTRRRGLSKSNHAWFIGYGPYNAPVEQQVLVTVFVEYGVGGSVGAAPIAKEVFKAAFP
                     PGSFRRTDRTDGYRMDDETPIEEIQ"
     gene            complement(25827..26348)
                     /gene="mreD"
                     /locus_tag="PLF66_01140"
     CDS             complement(25827..26348)
                     /gene="mreD"
                     /locus_tag="PLF66_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008594020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HMZ62665.1"
                     /translation="MILERIVIGVGILLAHILNGTNLFDIGTAIKPDFMILLVIFFAL
                     RKGGMSGLWIGFIGGLLTDAALGGEEGLGGKVFYKIGIHSLSFSLVGYLLGKFGRMSY
                     NENFISISIFAFALTLVARIFTYMLFSFFFHSNLNYSFIATSVYNAAIAPLTFFILSW
                     IYKLDPTSSEGLR"
     gene            complement(26528..27532)
                     /gene="mreC"
                     /locus_tag="PLF66_01145"
                     /pseudo
     CDS             complement(26528..27532)
                     /gene="mreC"
                     /locus_tag="PLF66_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002178830.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005618 - cell wall [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
     gene            complement(27545..28567)
                     /locus_tag="PLF66_01150"
     CDS             complement(27545..28567)
                     /locus_tag="PLF66_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010574148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HMZ62666.1"
                     /translation="MIFDNLYALFSNDMGIDLGTANTLVHVKGQGIVLSEPSVVAVQA
                     STGKVLAVGQEAKRMLGRTPGDIVAIRPMKDGVIADFETVEKMIRYFIAKVHNRTTFV
                     KPRIVIGVPSGITEVERRAVRESAEQAGAREIFLIEEALAAAIGANVPIHEPAGNMIV
                     DIGGGTTEIAVISLGGMVIAESIRTGGDEFDEAIIKHLRNQYNLVIGDRTAEDIKLTI
                     GNAYMDKRTDTMEVKGRDAISGLPRTLELDSNEIRKALKEPTDQILDGVKRVLEKTPP
                     ELSADIVERGIILTGGGCLLRGLEYFISRETGVPVFRAENPLTCVVLGTGKYLDELKY
                     IKRGVK"
     gene            complement(28654..30243)
                     /locus_tag="PLF66_01155"
     CDS             complement(28654..30243)
                     /locus_tag="PLF66_01155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62667.1"
                     /translation="MFNKHFIYFSFFLFLFFSFIFHTHIPPIWPDEVLFFNPAFELGI
                     NGVMRTSVLDGLIPGMDTHTLWMPPTFMVLLSLLLKVFPANLLTARLFSTFVSLGSLF
                     LVYQISKSLRFSLKRIGFILFFIATDLVFLKFSHTSRMESLCSFFALSAFYFLLKSKN
                     QTDHNELDPTKSDLFFSAVSLSLSFLSHPFGIVHSLPILFLLYQRNSFTPSRIFLYCI
                     GGLIPLTAWLFYIIPNLDLFVIQFGAQLSRKNELLGKFNWIDKVKIIFSVYKFPLIKL
                     SLYFVTVFMFAFYFIKTKFKDTNKAEYFIKLIKTDNEFFALIWLLTITTFLFLSSEFW
                     YVFHMTVPFVLILSYLLSEIKLSRYFVYLSIGYNVFIIIWIIVSSFFIYKLPEKVDIF
                     FSEIEKVVEAHNNVYLQAIPDPYFYLKEKFPNKNIREFIPGELSGLNKSQTEKKSKSL
                     LVELGVLKDNYKIDESFYYATIEKQDVFIFYNENLMNPYIKKHLEKNSAFFERKIISV
                     ETPKGSDLKLEAVAFVKKQKN"
     gene            complement(30270..30605)
                     /locus_tag="PLF66_01160"
     CDS             complement(30270..30605)
                     /locus_tag="PLF66_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004769168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HMZ62668.1"
                     /translation="MDIKTQNIGKHVLVKLAGRLDITQSDEVESHLAKDVLSGEGDII
                     IHLGSISYISSSGIRIFVGMVRELNRQGRKLKLCNITPPVKKVFDVVELLDLFDVYAT
                     EEEAIASLP"
     gene            complement(30750..31331)
                     /locus_tag="PLF66_01165"
     CDS             complement(30750..31331)
                     /locus_tag="PLF66_01165"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HMZ62669.1"
                     /translation="MAKSDTQSKKYDETVEDDSFFTPDQIIQIVKVLKSNYTHLLWFK
                     LLYTFGMNLKDIVNLKVGAIDFQRSKIKILGGEKSLSRSLDIPFSILCDFRIFCHQKT
                     SDDFVFQGRSGKLHTRTIQKALEKIENKIGLSVSVPKIRRSIAVHLRQNGWSQVEIGL
                     FLGHVNTRSTRNLFGQIRSFPKGDLVSIEQLLK"
     gene            complement(31724..32425)
                     /locus_tag="PLF66_01170"
     CDS             complement(31724..32425)
                     /locus_tag="PLF66_01170"
                     /inference="COORDINATES: protein motif:HMM:NF013640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HMZ62670.1"
                     /translation="MNYKVIVTLGMEELKDYKNPSLQDADILEIRLDLLSQSFIKEEL
                     SPILKSLNKSILFTYRNPKDSSENTNTKIEFKDIEKVLNEFNSESNYIDIELDQEESI
                     FDNISNSVFTVIYSYHNFFGILDSSQIRNWITKKRTQRCIYKFAVMPKSYIDLKIFLE
                     GIKENAEKNSIIGIAMGDIGIYSRLFGDRYGSIATYCCIGKPRAPGQIEIRNFYEKRD
                     FYKDKPVPSPDTFRF"
     gene            complement(32432..34390)
                     /gene="acs"
                     /locus_tag="PLF66_01175"
     CDS             complement(32432..34390)
                     /gene="acs"
                     /locus_tag="PLF66_01175"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012390366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HMZ62671.1"
                     /translation="MAKERILQPGKDFVKSANINLKEYQKLYNESIKSPQSFWKKQAE
                     RLTWFTPPKKILTANFAEAKVKWFEGGKLNVSYNCLDRHLNSSLKNKAAIIWESDSGE
                     ESKTFTYQELHREVCKFANVLKSQGVKKGDRVLVYLPMIPELAIAALACTRIGAIHSV
                     VFGGFSPESLIGRIEDCKPKLIVTSDGGYRGGKAIDIKKNIDLALQKSEVKIKNVIVV
                     KRTGMEADLNWQEGRDHWWHYLMKTASTECEPYKANSEDPLFILYTSGSTGKPKGVMH
                     TTAGYLLGANLTFHYVFDHKPEDTFWCTADIGWITGHSYILYGPLSNAATSIMFEGVP
                     TYPNPGRFWEVIDKYKVNVFYTAPTAIRALAKEGTSHIVKHSLKSLRLLGTVGEPINP
                     EAWEWYNKNIGKGKCPIVDTYWQTETGSIMITPLPGAIATKPGSATLPFFGIKPVLVD
                     NEGKEIKDKGEITGNLCISSPWPSMMRGVYGDPKRFKSTYFSQFKGYYFTGDGARRDK
                     DGYFWITGRVDDVLNVSGHRIGTAEIESAMVSHKAVAEAAVVGYPHDIKGQGIYVYVT
                     VKNGITTNDELKKELIAVVEKMIGKIARPDVIHWAPSLPKTRSGKIMRRILRKIAANE
                     FEGLGDISTLADPSVVATIIEDKKKYHS"
     gene            34620..35720
                     /gene="lepB"
                     /locus_tag="PLF66_01180"
     CDS             34620..35720
                     /gene="lepB"
                     /locus_tag="PLF66_01180"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000764508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HMZ62672.1"
                     /translation="MTQKSKETEKQTNIQQQSFLSSIGSLLFIIILVFGFKSSILDAN
                     NIPSGSMIPTLKIGDFLFVNKMRYSFRMPFTEKELYRFDDPKRGDIVTFAPPNRDKVR
                     ALLIALNLSPDLSVYIPPAIEENYIESNGPQRFFGLANMFSKRFVKRVIGLPGDTIRL
                     TQTQVKTTRGDAVSYTFIEYKEKGSTDFKNYGPQIQPSEKELSDLDNVNAIGKSLFTE
                     NKRDFKHFTIEGSHMGNYTFYEYCSRNGGVCTIPDGYYMVMGDNRDDSSDSRFWGLVK
                     REDILGKALIIYFSINWKDYTCMYKEGAGTESDPYHEEKYENEELFKHCHVSEIDRTM
                     MNESIFGWLDRTLRYRIWRMDIRWKRIGRILQ"
     gene            35672..35917
                     /locus_tag="PLF66_01185"
     CDS             35672..35917
                     /locus_tag="PLF66_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010570545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HMZ62673.1"
                     /translation="MAYGYPLETHWSNFTVNLWELSSLGIEFAFIFVGSVLLGNYIDE
                     KFNFTPWGIMLFAALGFSFGIYYIVYRANKLESDDKK"
     gene            35923..36375
                     /locus_tag="PLF66_01190"
     CDS             35923..36375
                     /locus_tag="PLF66_01190"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HMZ62674.1"
                     /translation="MNIQIISANLEDLNEVLPLFNLYRDFYEMKPDLNKAESFLRERL
                     QNKDSKIFLAKSENISVGFTQLYPSFSSLSMKPVWILNDLYVLESHRKQGIGKMLLDA
                     AKQFAKETGSKGLTLTTAIQNTTAQSLYEKYGFMRNDHFYEYFLFFNE"
     gene            complement(36379..37056)
                     /locus_tag="PLF66_01195"
     CDS             complement(36379..37056)
                     /locus_tag="PLF66_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016548480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMZ62675.1"
                     /translation="MFSGIQCINLNRSFGDPPIEILKNLNFEIPKGQFVSVTGRSGSG
                     KSTLLYAVSGLDKITSGKVLFSGESIHEMKEKDLNQFRNFKMGFVFQFHYLLPEISVL
                     DNVLMPARKTNLHKEKMEEAKHILEDFSLSHCIHKLPGQISGGESQRVSIARALIMRP
                     EYIFADEPTGNLDSVNGEKVLSLFQKVNKDYKTTILMVTHDAGYARLAQRTIRVSDGI
                     IESDTLN"
     gene            complement(37056..38306)
                     /locus_tag="PLF66_01200"
     CDS             complement(37056..38306)
                     /locus_tag="PLF66_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008595338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMZ62676.1"
                     /translation="MIFLALKQLFTRPQQTFLTFLAIVIGSIGYILFTSIMFGFQEYI
                     IEQLVNSDAQIRISPRDETITEETFKDVFFPNKTIFWIKAPSGKTDNSRLSNIQWWYD
                     KLNKEPGVVAFSTQLVRQVSFIQGRLIYPAKIIGINPEEQTKITNTDKYIVQGEKLKS
                     LNNGDSLAIIGANLKNKMGVRLNDIIQVGIPGKQITPLKIIGIIQTGNRLMDDSICYT
                     SITTLQRITESPGEISDIVVRVTDVSRAKEMSEDWSQFTKDKVESWDQANESIMSVFN
                     TQNIIRNTITVVIILIISFGIYNILNMVVNNKKRDIAILRSIGYEESDIVILFLVQGL
                     ILGVTGAIAGSIAGYFLAKFVSTIEVFKGKNLATGTTHLMVSYNIYIYLKGMFLTIIA
                     SLISSMIPARAAAKISPIEIIRGS"
     gene            complement(38306..39073)
                     /locus_tag="PLF66_01205"
     CDS             complement(38306..39073)
                     /locus_tag="PLF66_01205"
                     /inference="COORDINATES: protein motif:HMM:NF024112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HMZ62677.1"
                     /translation="MDKRKLFLGIGFAIIIIAIFSYLFLFKKQNTAKPQVGPIVEAIY
                     ALGTVKSDDIYNLKLGITSGLTKLYVTEGDLVEKGQKLIATESSDFTSPIAGTVTRIY
                     LDKGETIMPGISVLTVMDLNKTFVQVSLDQSSALRIRKDQKAELSFENLRGSKLIGKV
                     KKVYPSDGQFIVRLETDSLPEGILPGMTADVAIEVAKRDNVLLIPANAIHRGQVRIKR
                     KGKVEVVSVKIGAVNGELAEILEGGIFADDEIFLGRE"
     gene            39271..39552
                     /locus_tag="PLF66_01210"
     CDS             39271..39552
                     /locus_tag="PLF66_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010570611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HMZ62678.1"
                     /translation="MILSFKDKESEKIWNSEFTKKFPIEIQKKAKMKLELLNAAKKLE
                     ELKIPPSNRLHILHGDRKGQQSISVNDQFRICFVWENGNACNVEVVDYH"
     gene            39562..39879
                     /locus_tag="PLF66_01215"
     CDS             39562..39879
                     /locus_tag="PLF66_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012389196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HMZ62679.1"
                     /translation="MKKKINAELNIHPGEILKKEFLEELGISARTLCLETGIPESNLS
                     QILKGKRDITAHISLLLGKFFGLNDVFFLTMQNHYDITKEKMIHEIRLSKIRPYHKLK
                     KSA"
     gene            39979..42237
                     /locus_tag="PLF66_01220"
     CDS             39979..42237
                     /locus_tag="PLF66_01220"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HMZ62680.1"
                     /translation="MFSPKKRLLKIKNDGLRLTPIFCKERIGMLLRHCKECIYAFPTC
                     AIIFLFSILPISALDTIDINTCNPNCVIGKNLEILEDKTNTLTINDIRDPRRDKACLV
                     PADTTTEPCWKKSESDKPNFGISASAYWVRFKVANNTDKPVRYFLELYYPQLDYAHFY
                     YYQNGHIKEIVLGDRISFYKRTLPNRNPLFEIKMKAQKEGVYFMKFTSQGTVTFPLSL
                     WEPKAFYDYDHNMQMYYGFYFGILFVMAMYNLFLYFSIHDKVYLFYVFYLVGISSVAS
                     LQAGFAYEYLFPDSPSFINIYFFISIAEAGFGIILFTRSFLNTKIQLPKVDWILKVYF
                     ILFIVLFIGGVFLPYHSVIPFSALLMIITPTTCFIIAFILLRKGYRPARLYFLAWSLQ
                     IISTIMFAFRGIGLLPTHFIIVHGFEVGSVIEVILLSLALADRINILKKEKEESQKAL
                     IAKQEEAIAIQKELISSYARFVPEQLLTFLGKDIITKVGLGDSVQKDMTILFSDIRAF
                     TSISESLSPSENFGFINSYLETMGPIIRKHNGFIDKYIGDAIMALFPQKPSDAIDASI
                     EMLEELHKLNVKRKAKGYSPISIGIGIHTGSQMLGIIGEKERMEGTVISDVVNTASRL
                     EGLTKAYSTALLVSEDVLDGVADKDKYEFRFLDTVKVKGKNKAVRIFEILNGVSPRIR
                     ELKLQTKPDFEAGINFYYSKNFKDSIKKMKSVLKKDSKDKAAELYIERCNFYSKNGVD
                     PNWDGIEKLDFK"
     gene            complement(42230..42706)
                     /locus_tag="PLF66_01225"
     CDS             complement(42230..42706)
                     /locus_tag="PLF66_01225"
                     /inference="COORDINATES: protein motif:HMM:NF024212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HMZ62681.1"
                     /translation="MDKEQKKEQRILEVESWRSFLSFHADVMFQIEKEMSLAGLIPLA
                     WYDVLIVLEQSREKKRTLGELSKKCLLTKSGISKIMNTLEKEKLVTRYKNQDDGRSYY
                     AEITEKGSNLVKKAWVKYKECIYKYFIDNLSKEEQTNLRIIFQRLKENLSKETSFT"
     gene            42806..43246
                     /locus_tag="PLF66_01230"
     CDS             42806..43246
                     /locus_tag="PLF66_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004787521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HMZ62682.1"
                     /translation="MLQKLFTTDNSWIGLICRLTIGIMILPHGAQKLLGWFGGYGFDG
                     TMNFFTDSMKLPYIIALLIILGEFFGGVALILGVGTRFFAAWVGIILLSAMFIVHLEN
                     GFFMNWFGNQKGEGIEFFILVLGLTISLLIEGGGKYSVDSILFK"
     gene            complement(43439..44236)
                     /locus_tag="PLF66_01235"
     CDS             complement(43439..44236)
                     /locus_tag="PLF66_01235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62683.1"
                     /translation="MKNKILVSILFLFGIIRISAEEQSSFAIPLRVEPAAVYTKVRID
                     GQVSEKRDSNIYQREKVGYIEGEYKFLQYFSIKVGTTKSQWERSGSKTITQYDRLNVG
                     LKFAREHQFSFGAFVWGGGVRWFDRQSKQYARDGVAPELYLVRPNLNFGLKIGSFEAI
                     VDLQFQSETNRAFKENSQEEFRRYYQAGLALSYGVTDKLRLFAETEYRKPYNPVVDVN
                     TKFWNVYPGLSYQIYKGGYLAVSGQLPVIPERLMDRGIRISYFHVFE"
     gene            complement(44226..45065)
                     /locus_tag="PLF66_01240"
     CDS             complement(44226..45065)
                     /locus_tag="PLF66_01240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HMZ62684.1"
                     /translation="MKFISILLFIIFLTNCDSKKHDMPKEFRSQFLCYVGAICPTQRG
                     VKYGILGDSWTDLALGVPLIESLRVQLEKNYNYKMVGSTLAGQTMATVYNTALYTKVI
                     DDAGPDIKYMLLSLGGNDIQGNPSAYVGRFAAEKQARFNTVQNLLLNLIRSGNAYKVQ
                     KYGGAPMLWIIHGYDYPNPDVVSAPGSTGCRATLRSAGFTDTEINTLEVDVLSDYNNM
                     LRDLTTREPQLRYIDLRRTLGGPPVAAAGNMWDCIHPNTGGFSLIAKRYVSVLEGYTN
                     YEK"
     gene            complement(45078..45545)
                     /locus_tag="PLF66_01245"
     CDS             complement(45078..45545)
                     /locus_tag="PLF66_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004780314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HMZ62685.1"
                     /translation="MRPFKVLGIQQVAIGNENKDKLSKFWGDIMGLTKIGNYKSEKEN
                     VDEDIYRIGAGHFAVEVDLMMPIDANKSPKVHDPKLNHIGLWIDDLPACVKWLGEQGV
                     RFTPGGIRKGASGFDVCFIHPKGNEEFPLCSEGVLVELVQAPADVIKAFNSIS"
     gene            complement(45804..46679)
                     /locus_tag="PLF66_01250"
     CDS             complement(45804..46679)
                     /locus_tag="PLF66_01250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62686.1"
                     /translation="MFILTFSISLNAEDFRIIYSSSLSGNLYSCICGLKLSVGLAKRD
                     TFLKQKGINSSKDILVDTGNSLDVKSKKNKTEAIFHSFFKMGYVSVGVGINDLQEDLV
                     SIVDSERYPLNSANVFIKKFFGSSKVGKPISIIKKGNKRIGIISLTSPTIQYTLSGEL
                     KRKFIIKGLESTLQNIISIDENKADLYILLLHGSTDEAVRLTSLNKNFIVIYGDESIR
                     KPVSNKTGYLEVNGVKVYSTFDVLGDKIGILNLTETESGYKIKSSEVIEMNVDKLTDS
                     DEILSVMQQYGIKPE"
     gene            complement(46843..50535)
                     /locus_tag="PLF66_01255"
     CDS             complement(46843..50535)
                     /locus_tag="PLF66_01255"
                     /inference="COORDINATES: protein motif:HMM:NF021606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HMZ62687.1"
                     /translation="METLQGDLKHLRAKLDLAVQKYEKNLKEEQTSDTALEPELTSPI
                     TESYEAIQEEKLEPQPIPKETKPTIIRPSLLQNNSTQKEEVTYKPRPVSQVKETYSRK
                     ESSPDFIDDLKSWFQSKVGNIPLEEFLGVNLLNKIGLLLLVVGFSFFLRLAYDWIGPF
                     TKVIGMFVLSIGVYFGGDKIFRTKKYSTFGLGLIASSFALLYFTVYASYNIEATRIFP
                     PDQRLIGFILLILTSSFVIGASLKYNEEILTSFAYFLGFLTISINENSDFNYFSMATV
                     GLLAVSLIVIMTIRKWKYLTGVGIIATYANYWLYASGLPRTENGYLIKISETGHNYYV
                     ESILYLLFFWIIFFISTFTMKVVDKKTERVCSAINIVNAFSFFVMLGYVKPEPSEWGP
                     FIVNMSLGTIYIIGAFAGKKYKRDFLWNSSLVLGITLITLAIPSKFSEYGHVFGWLLE
                     SIVLIGLGFVYREAYLKNLGYTVLVLNLVRFFTLPYGAVYIFESVNFPTLYFDFNRGM
                     LYVLMILSFYGMILFSKKFYSAWSKLDKFAYNGFGLFATFTLVLFSIDLIQKPYQAFL
                     LIPASGLLLYISLLSKNRNFYYSSFGLMIISLLLSIPLFFKSSPEINQIDQAISFSLV
                     LVGSSIQYFLAKENVLLRRYSHILSVNIIDQRQVPFLKVLFFKGETFLWVSLFSILGL
                     VLKDFSPYLHSIVLIGIVCLFFLVNYKYQQGLKQGSLLSGFIFLVGIYRYGLYREDVK
                     LALSDKGLIAFTNLFPFWISGIFVLFNSNKIGITLEIKKILHPIIISATTFSAMLIGD
                     IFIDPPYRLTYAAINSLLLVLAYKKEENLIFIYNSLLITFFTLFLGIADMVFSKKSPV
                     GLYEYMNLVTYTITSFGTAGLIYIFTLDLFSRIFSYIQSILIGFCFILVVVPVEYRSI
                     AFAILHLGHLFFLFRFQMQKYYDYAYLTLLISVLYFLNHTNFPSGSIQEDPTRVALII
                     GYSVLSILQTVFIFAKDTLSRNRIIYYILTISILFWSIYIVSEIHFSLFFFAIFVYIS
                     AFIIQKYHFPEVLHSYYYPLLLTMVYFLYMTLGNNSNVSGGMNVKDFTIGTFTLLLLL
                     ISVKNVETYKSKQTDFIPNKYSFENIVTLSLFLEFILLTMTVVDVRFWSLIWTLEAIV
                     FIGYGVYRNKTLIRYAGIGLIGLAVLKIAFWDLQNLKENVRVLVLISIGGLFVIASFV
                     YARFKDRLFSNSEDNKGLPQDNDPKN"
     gene            complement(50593..51291)
                     /locus_tag="PLF66_01260"
     CDS             complement(50593..51291)
                     /locus_tag="PLF66_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000373608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HMZ62688.1"
                     /translation="MNQSYSMSPAQVMAEQQRFIVKVYSWMSVALGLTGIVSMLVASS
                     ETALRIVVGNSFVFYGLLIGEFILVATLSGLVQKMNATVATFVFLFYAALNGLTFSII
                     FVVYTSASISSTFYITAGTFALTSFYGYVTKSDLTKLGNLFMMALIGLILASVVNLFM
                     KSEAIYWITTYIGVILFVGLTAYDTQKIKNMNIIGNEGTEEDQKEAIMGALTLYLDFI
                     NLFLYLLRIFGRRR"
     gene            complement(51303..52130)
                     /locus_tag="PLF66_01265"
     CDS             complement(51303..52130)
                     /locus_tag="PLF66_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002102649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HMZ62689.1"
                     /translation="MEEITKFLEPFFIPREVPATIIKGIMEMIYKVEVTGSEHVPSKG
                     GAVLVCNHTDSLDIPVIGLYIPRKVVFLGKNEVFNPQEAIIKMMNAKNSPLVSPPFSF
                     LKEGLEKYLNTIGDVYSHQIQKWGGMPIIRNYHGEDAKSAVAYYEELENYMVSILKSG
                     EIVSIFPEGTRTLSGVMGPFKAMAAKIAIRANVPIIPSGISGAWNMSTPQAILSGQTR
                     NTIIKYNIGVPILPDEFPKEAEKKAAKMLTEELEKRVYFLTTHPERRGKPRRFATKL"
     gene            52322..53740
                     /locus_tag="PLF66_01270"
     CDS             52322..53740
                     /locus_tag="PLF66_01270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62690.1"
                     /translation="MSEIKTFGELKKSNYIERSIKIEMAENLSAKLARKENIFPKIHG
                     YEDTVIPQLVNAILSGHNIVMLGEKGQAKSRIMRALINLLDEKIPVIKGTEIPESPFH
                     PITAKGKKIIKEVGDNTELVWLTREERYGERLAPGARIGDIIGDLDPSRIVNGEPLSS
                     EGAVHFGLLPRMNRGIFAVNEMPDLDYLVQVSLFNILEEADIQIRGIPIRFPLDVMVC
                     FTANPDDYSRSGKIISQLKDRIGSEIRTHYPKTRQIGLEITRQEINLPLKTPEVIVPP
                     FIEEIIEEITIQARSSHLVNQKSGVSARLSIANYEVAVSSARKRALHFADTTGYVRLT
                     DLGNIFASCSGKIELDPYRDEAVSEYQVVLKLLEQATRVVFEEYFPPKAYSQNFNDIA
                     KQIYALGKLEISDLMPVNSYRALLKTVPALWDLLHEKGMDIEDHLFVTGVEFILEGLA
                     STQKLSRRRLGEISTFKTIDAY"
     gene            complement(54127..54594)
                     /locus_tag="PLF66_01275"
     CDS             complement(54127..54594)
                     /locus_tag="PLF66_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004787222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF327 family protein"
                     /protein_id="HMZ62691.1"
                     /translation="MQFSINTDKPKTIADKNSKGIKSDSSSKIKDKENTFLSILESIV
                     PSDKEETREINELWQKLPEMEKRFLNLPSHENLTEYKKLVKDITNSILKNNTQLVQAK
                     QRGRNDKKILMTVKIIDENIQILASTMLSPQNSAFSLLKQLEKIRGLLMDLKE"
     gene            complement(54594..54932)
                     /locus_tag="PLF66_01280"
     CDS             complement(54594..54932)
                     /locus_tag="PLF66_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010571539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HMZ62692.1"
                     /translation="MNEEQEEFSVNSIIGEGSEFYGEFKLSGLIRIDGKFRGLIETDG
                     KVIIGKTGIVNTDIKARVVVAGGEIHGNIYASERVVLLSTCRLYGDIITPKLFIEEGV
                     LFEGKCTVNP"
     gene            complement(54942..55928)
                     /locus_tag="PLF66_01285"
     CDS             complement(54942..55928)
                     /locus_tag="PLF66_01285"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012390384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HMZ62693.1"
                     /translation="MKFKIKEYIQSIYKKIQEELNSLKLFLIQSKDRIIKKRKEFLTV
                     MVIPHNEKPSFKISITYKAITIFFLIIGGLIGTSAIIVLGHSGRVHEMEELKISSGDF
                     QKQSLKLKSEMNLLHDISSYYHQRVSRLYIKLGGDPNKVGMTDKSKLPLGVFDSNSDI
                     IPETYTLKSDIYYLKKSAELTKEIIKTIKQRKSIIKNTPSLWPTRGYILFPFGKYFSS
                     ITGREVINNGIDIGTFPGTEVVATAPGEIYETGYTESTGYFIKIIHKYGWKTIYSNLE
                     RLQVKKGEMVSKGDILGYVGKTSGSSIFHLHYEVHVGTNPLNPYSFLNQVQN"
     gene            complement(56027..58354)
                     /locus_tag="PLF66_01290"
     CDS             complement(56027..58354)
                     /locus_tag="PLF66_01290"
                     /inference="COORDINATES: protein motif:HMM:NF017106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P83/100 family protein"
                     /protein_id="HMZ62694.1"
                     /translation="MKNIYLITIVFLLQATLYAQSKVVLGEEEIRKSPYAKFENRSDK
                     FATPELKAIEAEIGKGLHNLVIQTDKPINYKGFTLQRFYSDEKGKFGGDILALNKNAS
                     YGHINSIKRILNAYIQSSFEYTEKNADVLVMEVLYYNAQNRNKLDRIKKRYTKSLVAA
                     LTKNKVGISRKFSEWPGNTQIVIPIKYSEDPNKIPEVSENKPKEEKKEETTTTTTGKN
                     PVVEKEKIKQIKEKIPSSDIPKENITETKKKPDDKKNKTDGTVIKEEKKIIEKKVELV
                     EKAKENTTDSKKTKTDEDPKTIEPKKAENSNKPQEDSKSSKESAKEAVKESKEPAKET
                     AKEEPKTTKDSKDSKTVVINEAKSEPKKAANEDKPKEEKSNSNTTKDAKLVETVKEPT
                     KDSKTANPTKEAAKESKEPAKETAKEEPKTTKDSKDSKTVVINEAKSEPKKAANEDKP
                     KEEKSNSNTTKDAKLVETVKEPTKDSKTANPAKEAAKESKEPAKETAKEESKTTKDSK
                     DAKTVAINEAKSEPKKAANEEKPVAKESKPVEPAKKVEEPKKEAPKTVVAPPAKKADE
                     KLPTTKEEIKKMQKELTELKIKEKDKVENSENVVGEKIVFLKVKEFLAEGHYSSEMWA
                     VDTENDDELFQSPFKKVCGKEFKELGTGVVVMSYKANDVLNSDTHHLALLDKDSLKLI
                     KQTNEVIFWRSFLKEQNNKIYAVEFKDGKYYLAQFKDDLTFEMRSSDPINPDCEIAFK
                     KNKIYLTKRSQDNNEVSIRVLNRDNLKLRDVKVMK"
     gene            58458..59426
                     /locus_tag="PLF66_01295"
     CDS             58458..59426
                     /locus_tag="PLF66_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020769592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HMZ62695.1"
                     /translation="MSDILNKVKKAIAIEVNAIKHFQDTLDPNFESAVRLILDSKGKV
                     IVTGVGKSGDIAKKISSTLSSTGTPAIFLHPTDSAHGDAGLIAEGDIVIAIGKSGESD
                     ELIALIPTIKKIGAKLIGITANPKSKLAAACDITLITPILQEACPLALAPTSSTTIAL
                     IAGDAIAMALMELKNFQADDFALYHPAGRLGKRLSLAIEDVMRSDDQNAFVTPDADLS
                     MVLNEITTKRLGAVAVIDSEQKLIGIITDFDIRKSLTDGNLTKEKKAKEIMNPNPSSF
                     TLGMKAYDVLVAMEDRKHPISVAPITDKQNKLVGMVLIHDLLQKGL"
     gene            complement(59513..61942)
                     /locus_tag="PLF66_01300"
     CDS             complement(59513..61942)
                     /locus_tag="PLF66_01300"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HMZ62696.1"
                     /translation="MRFIRKLISSFHGRFVLREQIIIPFILEILFTLGIVWGFLHWNS
                     EKTAYDLTEKIQEQIQTRTKEYIRTYFFNSRNLIQQNSQAIKFGLMRPGVNFNYPLYF
                     MEQIKLYPDINDIFFAEKNGRLHAIERQKDKYIFRETNEQKIRFFYLLNEKGEKQELI
                     KTQSYDTVNRPWFREAVKNPNMILTNNVFVQANTGLLGITIFKSILDEKGILVGVSGV
                     NILLTNISNFLKKNLATPNSKVFIVNSKGEILASSVQMQTLKIIDDNNFILYKLNELN
                     DGLLVEVAAQIQKLKSNRNFTLDYHGIEYWVQYHDISESGIQWYMITIFPDSDVLGKI
                     KLNNRIITVIVMIAIMLMVTIGIYSSKLILAPIQELNIVAKNLSLNQFNESHIESLSS
                     LKREDEVGELSRSFYKMAKDLRILFENLETKVIERTKELEESRKLSESANRAKSLFLA
                     NMSHEIRTPMNGIIGSLQLISTENFSKENLEYFESIRISAQNLLVIINDILDFSKIEA
                     NKIELEKISVNINQIIKDVISIVKFDADKKGLFINVEQDIDLPVELEGDPVRIRQIFL
                     NLLSNSIKFTFKGGIKIKSKILEKNNDSYLIQFIVEDTGVGIDDNKKSILFNSFSQVD
                     ASTTRKFGGTGLGLAITKRLVEMMSGEIKVDSELGKGAKFIFTLKLDASKKNEVAEEV
                     KSIKSDQIRKDINVLIAEDNDLNQKIVSALLKRLSLKADIAKTGIEVLNKLKEKNYDV
                     ILMDIEMPEMDGIEATSKIKSDQNYKDVYIVALTAHALTGEKERLLKVGFDEYLTKPI
                     HLEDLRRLFSK"
     gene            complement(62004..62459)
                     /locus_tag="PLF66_01305"
     CDS             complement(62004..62459)
                     /locus_tag="PLF66_01305"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HMZ62697.1"
                     /translation="MDPIHKIQLTDLETVIQKIGYNKEILINTVILNQIKENWGDIIG
                     PVFVNQAQPYKVEGDVLFLRVAHSAYKMEIGFLKDSILKAANKLFASPLLSKLTITTG
                     NMQYTPPQKATKQIETLEGKSELVETIQKEDDEFIREKLLNFIKKLKKE"
     gene            complement(62460..63557)
                     /gene="recF"
                     /locus_tag="PLF66_01310"
     CDS             complement(62460..63557)
                     /gene="recF"
                     /locus_tag="PLF66_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020765150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HMZ62698.1"
                     /translation="MFLSKLKLLNFRSHEDIQLQFKSKLIFFIGENGAGKTNIIEAIN
                     LFATLKSFRDNSDEELINWKQSHYFVKGEFSSEETNKVIEIGYSKGEALKRKVKLNGD
                     VIQKKQDIIGEMRAVVFSPVDLKIIDGGPSERRRFIDGFISTVNRNYFANILDYNKIL
                     KHRNTLLKKKDFSQSELLPWDQLLVKKGTDISKERARVIAGLDIIYRENLSKLSGNKD
                     DFHITYKPNVSLEEDYAKKLSERISRDTHLGYTSVGIHRDDIFVGRDNKDIIEFGSQG
                     QKRSTVIALKTSQFQFIQKEIGEAPILLIDDVIRELDVKRREYFVSLLMDCGQAFFTT
                     TDLEGIQDYIGNLQESKEVFLIQDGNISQVE"
     gene            complement(63675..64793)
                     /gene="dnaN"
                     /locus_tag="PLF66_01315"
     CDS             complement(63675..64793)
                     /gene="dnaN"
                     /locus_tag="PLF66_01315"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HMZ62699.1"
                     /translation="MKFKVKTNDFQKAISSVEGVITVREIKSILSNIKVEAEDGKVFL
                     SATDLEISIKTSVNADTIEPGTTSIPAKQLSNAFKTINFPEALLTNDNAEGNVSTTIT
                     DSEKKKDFKMNINGVEGEEIKTISKVDDSLVVDFPCFTISEMIKKTSYSVALEDTRFV
                     FNGLYVISNEGKISFVGTDGRRLAKIDRVIPNALPFSKGVIIPHKAIKEIVKMIDSND
                     SGKIGIVDNQIYLKIGNVELLCKLIDGNYPDYEAVIPSKSKSSVRINKDELQIALRQA
                     LIAAEEPSRQIRMTFSENLLHINSSTPGSTEVNVSLPVDYKGEEMSIAFKGDYLADVI
                     KSIDDAEIIMEFTSSNLPAVFKDPSDPQYTSVIMPMKI"
     gene            65403..67430
                     /locus_tag="PLF66_01320"
     CDS             65403..67430
                     /locus_tag="PLF66_01320"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HMZ62700.1"
                     /translation="MTYISISSQLIDATTKIVNILRQIKDINSTTKLIAINAAIESSR
                     SAQLADNFSSLSEQVRTLSTRSEESFSEIQILIENLRVCCAKAIAVRLADLSIDIIDK
                     IDRNLFERNCDCQAWATFEDNVNACLTGNGQDASSLLNNLVNVYEVYHDIFLLNKDGY
                     VIATGKNPQLLGQNQSDRVWFKEVIRNNSVHVTDMYFSDSVGNHTVSYSAPVRDQNKN
                     IIGVLSTRFNWDYVYDIIEKVKLESHCKIFLISSVGTIISSKQKYDILRDNMLWLGGG
                     ESAIQGKRGYSIETARNGQWKAYGFSRTKGYNAYAGKGWSVIVDEPIHLENPRVIIES
                     FSEISENKIGTRSRAHSEDVNNQLLQITHQLGESIESINKINNVTTTLALNAAIRADR
                     AGDQGRAFSVLAEEVRAFSQKSDQLTEEINTTLDSLNQVVRETVSTRLADAAFDTIDK
                     VDRNLFERNCDVQAWTTFEEVINCAETGINREETSALLKDLHTIYEVYYDIYLLNTKG
                     VICAAAIRQDLIGQDQSDRDWFQKAIQGYVTVTDMYKSDTIGTYTVSYCAPVKNKNGQ
                     IVGVLTTRFNWNFIVDIINATQVYSDCKVYLLNSMGQVIASQDNREILKRDFSKYEAY
                     KLAMEGGNGFIVERDADTNQIYLVGYAKTEGYNKYQGKGWCVLILQQKRFE"
     gene            67439..68428
                     /locus_tag="PLF66_01325"
     CDS             67439..68428
                     /locus_tag="PLF66_01325"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HMZ62701.1"
                     /translation="MSTELTTTNLELESLSENKNFIFLFGAAYFSIQLTSVVEVYDSP
                     EIKNLPWQELGLKGMIEYRGIPVPVLDPLAIANITSDRGNKPIKTVVIFEKENCKIAL
                     AVDKFHNIIPEPENESEDFQEDSFESFIIGFSMLSEKPLVYLNEEKIVKHYKKIFTRQ
                     VSLKSNQKQEIFSTEKKQILEKFIFFTIGEVNFGVPIREVLEVLENLDVTPLFKVVPT
                     LRGLINLRGKVVPCMDISGYLGLPARSLNENTKFVLLQSGGSEIALCVDSVSKMKEID
                     NALIQNNEGILNGPISEYATGVLQLQKQTLLMISAKNLIESNDLKEYFLPEAE"
     gene            68429..71527
                     /locus_tag="PLF66_01330"
     CDS             68429..71527
                     /locus_tag="PLF66_01330"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HMZ62702.1"
                     /translation="MYSEEILSLWKEDKPAENPLIEAQSRKLFKQFIGKSLNDVQTIL
                     NKVAGLEISKNKQSDLDSVYRINHTMLGFSKILRIQKLTHLTSILDFIFDFARKENSV
                     SKYSIDYLIKLILNVEIKILNQLSDSGFIKEDISLLVEEAKVYLSKPLEVWNEKQKQS
                     QIVFAKKSYTIPAPYSSEVKTSEEDNQNKPPAESILESKQNLEVSIGKESQEEPINDE
                     PEDLNIPIEKVSLISDFYEECYENLSKIANRLVDLESTPDSIEIINELFRYVHTVKGG
                     ARLLKIVKIEKLGHSVENLLDLLRNQKMEVTPVFIDYLLEARSAMSEMIEEVASKGPI
                     RTRIAPLVRKLNDACGISSSKPKAAEAKANQDLPPKPQVSLDKGTKEESVSVKQEKTI
                     VAQSEKIPSPVVSKEKEPVVRIKERQVESIRVSIDKLDDVINTASELSISRIQFQEQI
                     ANINRMTRDIKRSLANAEAFDSKRLLDRISKSNQCLIADIKAEILKGDGDINESILTD
                     LVYRFHNEMKSEIARNELSQQEELTLILISFQELKNGMLKNVENLENLTSKLQNEVMN
                     FRMVPISTLFERFPSLIRDMARQAGKKVKMALYGQETELDRVMINTLLDPLLHILRNS
                     IDHGIESTEDRIKVGKNETGSINLSAYYQGSYAVIEIKDDGKGIDTDAVLKRAIERGL
                     ATEDRITTLSQKEILEFIFAPGFSTAEKVTELSGRGVGMDVVMSTIKGLQGSIDIQTK
                     KGQGTTLLLKIPLTLAVVRVLLFETGSQLLAFPMTNVDEILTISRDEIETVGAKMLYH
                     LRSEVISLVPLSDILNTQQPVFFKEEIPVIILSDGVKKVGLVVDALLGRQEIVIKNLG
                     NLLKKVPFIMGCTILSDRRLVIILNPREIIDIASDEKNDLDKKTPSLKILKKEEPQSI
                     LVVDDSPIQRKTIKAILTRAGYAVDEAENGFDAMKMVRVKRYALFCVDLVMPLMDGFD
                     LVSRLKNLPLYKSIPVIVITSKNSVEDKERGMKIGASEFLEKPVDPDILTDLVKRYIG
                     SE"
     gene            71527..73548
                     /locus_tag="PLF66_01335"
     CDS             71527..73548
                     /locus_tag="PLF66_01335"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HMZ62703.1"
                     /translation="MEEINYKNTNYPIYLCVDNILLTEYLENELSYNKFKFEKINFPD
                     LSLKISELRKSLIILQTDEDEYHLIELARRIKMFYGYETRIIFLSADYKTEEDASNVT
                     DKFFQIPVMFKEIENAIQKFLTDTHKILVIDDSKLVHNHLVPPLVNEGYKVYEAFNGR
                     EGLDLTIEYKPDLIICDIEMPNMNGFEVCTEIRKHPDVRDTYIIMSSTLGSASDIQKG
                     FNAGVDEYITKPVVIGELLERINRHFKQSLSGRENIIILEKDEHIIANITKSLRKQGF
                     SVRSTSTIEETIHLIQKYPFDLVISEMELGTETAMDLCLSLKNANTGFQPSILVLTSR
                     DSNADYKMIMNMGVSGIISKPFSMDTLLASVERLLADRRTAAERKQLLKYLSKSSAKI
                     ASEKAILTNGKGGARSEKKYASLLFTDIVNFTARCEKYPPDSVVEQINTIFELITKTI
                     HKHEGDVDKFMGDACMAYWIGGEHKVSALKLIDAVQEIRDKIQEANLKSEILTKDPIR
                     IRYGMNTGEVILCDIGSSEARIDLTVIGDHVNLAARLESASKQYGVESLLSEYTYALV
                     SDEVEVREIDKIKVYGKNIPVVVYELLCKKERLTIEQRELLEIYNNAKSLYFIGKFAE
                     AKKYFIQANKLEEIFPGRQTNPSKVYAGRCDFLLKNPPKEWDGVWALRK"
     gene            73597..74637
                     /locus_tag="PLF66_01340"
     CDS             73597..74637
                     /locus_tag="PLF66_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008597013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HMZ62704.1"
                     /translation="MKFSIRKPVLLFTLFFQNCIPYLYHVSTEQVKIILNRKPIPEVI
                     ESESEEGITRSKLKLIQKVREFAVKEIHLNPKGGFEYFTKLNREEVGWNVSASYPLKF
                     ESYTWWFPFAGTVPYKGYFDLKKAKEEEQTLIEKGFDTRVRITAAYSTLGWFSDPVMS
                     PQLRMSEDELVSLVIHEMSHATVYFAGDSNFNESYASFVEEKGTELFYKTFHSEKAND
                     VLEKRKKAKVQSDFIISQVKQTAFQLKEMYEKEDLSDEQKLEKKKQIIEDYRERILAN
                     SHLLTNFNPEKFKKTPLNNENFIGILRYHSGSKLFQDKFIEVGQDFKKFHQAMEELKA
                     LSVEERAGLLKE"
     gene            74669..75178
                     /locus_tag="PLF66_01345"
     CDS             74669..75178
                     /locus_tag="PLF66_01345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62705.1"
                     /translation="MSRFLKPRDLTTKEFHYLDSIFKKNRKTLKITFYLLILINWILN
                     TLGIFLFVIVFRERSFLLLIPTILLLLFVNLGFNPLFKMCKVGMKDKFQPDKVYFMEG
                     IFRLKWIGSWRPRLKYFIENYEVIVPSKWLSEIKQGEQITAEYSTLHPEAKSKKLYIL
                     SLNDRFRID"
     gene            complement(75175..75603)
                     /locus_tag="PLF66_01350"
     CDS             complement(75175..75603)
                     /locus_tag="PLF66_01350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62706.1"
                     /translation="MKYFLLLLILFNYGYSPLSAKQKSHKNKSNCEIWIGKGRGSEDP
                     NLSLELSICFHKNNVTGTAIWKSELGGTCKTTVSGFKNEKNTLFILKDLKFLEKSEKN
                     GWQICLTDKYRFFANKNKMNGFYESKACQDYGSITLEKKF"
     gene            complement(75647..76996)
                     /locus_tag="PLF66_01355"
     CDS             complement(75647..76996)
                     /locus_tag="PLF66_01355"
                     /inference="COORDINATES: protein motif:HMM:NF017183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HMZ62707.1"
                     /translation="MNSNKKLLGIISLAREILPTSSSISIQYPTNLIFYVNAKVNITP
                     TVQGSISSYSISPSLPSGLSINSSNGAIQGIPTSVASATDYTITAGSLTATVNLQIKN
                     TTSSRVYGQLGDFTTGTSNKGGVSADSLNSPRNCAVDKDDNLYIADTNNNRVLFYLKG
                     STTATRVYGQLGSFTSNTANNGGRSADTLNSPRGVYVDANNFVYIADTTNDRVLAYEN
                     NSTTATKVFGQQDGFTTGTSNLGGLSAQSLNSPRGAIRDKNNLLYLFDTDNHRLLSFP
                     DTSSTANDVYGQDNLFTTNSSGLTSSKFNAPFSIVFDSSNNLYVADLYNNRVLFFPKN
                     TKTASTVYGQQGSFTTSIANNGGLSSISLDSPIGVQIDRSNGLYVADGGNNRVLYFPE
                     NSTTATIVFGQNDSFTSNTANNGGRSALSLNNPRGVCIDSAGGLYVIDDDNNRVLYY"
     gene            78089..78556
                     /locus_tag="PLF66_01360"
     CDS             78089..78556
                     /locus_tag="PLF66_01360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62708.1"
                     /translation="MYKKLFFVSVGLWILALIILGKFFILGNAHPSADGRKYIELSKE
                     EKKFVLSEMRGLLTSFNGVLVGLSTNDKNRMIESARIAGTKGHNTSEGNHKTIMLKLP
                     IEFKKMGMNLHKDFDLLAESIENGAEEKEIIQKLANLSGQCVQCHAGYRFSAE"
     gene            78576..79820
                     /locus_tag="PLF66_01365"
     CDS             78576..79820
                     /locus_tag="PLF66_01365"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003001149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HMZ62709.1"
                     /translation="MSVVKEYWNRVINIGIEPSLHEKDAKYIRLTNAISVITSVWLFS
                     TIPSLLPLYPSSKFLILNSILFPILWPFSLYLNHKRFYILAKLFYSYTALICISLNAL
                     QVGREADNHLFLLLLSIFAFYSYPPKQMKYISLVSLSSLFCFIGIEIYLTSHPPILEA
                     PPEFFQIARLISLVALCVLVYVLTLYNYKTLNNAQDLLEIEHKKSETLLLNILPPVIA
                     ERLKVSNSVIAERTNEATILFADVVGFTVLSQTMEPEKLVALLNDLFSEFDTITKSYH
                     LEKIKTIGDAYMVAGGIPEPRKDHCEAVALCALEMQAIMHKGISRDLQKFRVRIGIHT
                     GPVVAGVIGKSKFIYDLWGDSVNTASRMESHGMEDKIQVSKEVYLKLKDSFEFENKRE
                     IQVKGKGLMETYFLIGKKAGVD"
     gene            complement(80120..82384)
                     /locus_tag="PLF66_01370"
     CDS             complement(80120..82384)
                     /locus_tag="PLF66_01370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62710.1"
                     /translation="MKQIQILLLCFLFTNCLKVKKSAFDFSSPVGLIFSLVWANQSQG
                     YSVGGTITGFTNGTLILQNNGKDDLTLTAPLTQYSFSGLTSGTSYSILPKTHPSGFSC
                     SIDNAKGTVVNNITNVNIICASATPTSLYSTGSYWLDYVKSDGTSPINASGTACTGSE
                     TGFYNACIHAAEFKKISIPNISSCDGIAATDLLSSFNWKCVVSSDNSVYIVSTGLKTD
                     KGLGNLIDFTSSGSWKNNSVTISLNNNVFLITNASQWWNNPIVLNNTGGSLSSAKTIY
                     IINNTSSASTYTFTQNNIGLVAQPEITKLSSGLPSLALGTYSFLWVEGKFDSSAANIV
                     LSISSGSKFNMIRNFRARTSGATTIYSVGSSSLYHNVGGTNTGGGFLILVQSKNILKN
                     IQMYNSSSFNLTVSSSDNIFLDSISLNSGSLGFTINGSYNNNFFANITSVNSTSSAFA
                     WANNFQSTSMNLAAANTGGTTSAIDNSIVNSPKFINTVAAHSGNTSQYSESNTVSSLF
                     YGIVKVSGSGTCTVTAATSPGINASCSKISPSETSPTTVTNITLASSFVGVTSDTKNL
                     SNTSGSQSFASITDWFNFDNQFRGWGKQGTFPTSALTGSCTTGTCSIWDLSLKNTDTI
                     IRNVNSCPNGSIIDTHLWSDSTAPNQAYCDANYKGSKLNGTTCITTFLRNAVEVFADG
                     VGNENGFCESNEECIFTPNIGAYQGHSSDSSNTTKLIKADTTSSTNTINCSDIGTSGT
                     ITNVKLWKYDTNGY"
     gene            complement(82512..83237)
                     /locus_tag="PLF66_01375"
     CDS             complement(82512..83237)
                     /locus_tag="PLF66_01375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62711.1"
                     /translation="MKLLIFLIPFIAFTACNRVNSPNDTLTKEKLTSIHWLENEFSNM
                     GNFLIFDANNKYTIEFYGEGCGQRWIGEYELLDSKRVLLKTGIQQTKDCQPDEPERTC
                     EYKKVPNPVLSLFYLDCGKTVFFNVSEKIQSGAQVQFEGVSVTALSGITGVTTDDAKL
                     REKPDVNSKSFSCISEMAPDWIPYVSKNTNLILLFKTNEKQKVKNWENYWYYVTVNGG
                     WYSRCNSKDNQGWIFGEFIKEGN"
     gene            complement(83527..84666)
                     /locus_tag="PLF66_01380"
     CDS             complement(83527..84666)
                     /locus_tag="PLF66_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004787889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HMZ62712.1"
                     /translation="MKLLRYLIPHMQESLNKKMVFLGGPRQVGKTTLSLQFLKSPSIE
                     NPAYLNWDRNTDRIQILKDQFPLQENKILVLDELHKYSKWRNLIKGLFDKYHTKNQFI
                     ITGSARLDYFRKGGDSLLGRYRYFRLHPFSVMEMNKNPNKNDLDILLKFGGFPEPLFS
                     QNEKEHRIWQNDRMIKVASEDIRDLENIKDISSMLLLAEMLPARVGSPLSLKSISEDL
                     QVSQPTVDRWIEILSTLYYCYTIAPFGSPKIRAVRKLKKLYLWDWSQVEEYGFRFENL
                     VASHLLKYCHFITDTEGYPMEIRYLRDTDGREIDFVVLKNKKPLFAVECKTGEKALSP
                     HISYFQARTEIPEFFQVHMGTKDFGSNKTGRVLPFVKFCKEMGMI"
     gene            85071..86627
                     /locus_tag="PLF66_01385"
     CDS             85071..86627
                     /locus_tag="PLF66_01385"
                     /inference="COORDINATES: protein motif:HMM:NF017106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P83/100 family protein"
                     /protein_id="HMZ62713.1"
                     /translation="MKYILILIAWLFFINIFAEEKTKLDEAEIKDSEKIDFQNKTRKK
                     AASETKLLHESIGKTLGSMISKDPNEMHSQEGVRVVRVSTAEKNKIGADLLFLDANTK
                     FGHINSIQRVLSGYIQTAFSYNNAKADILATYVLYYNGMHRGDVRFFESKYTNALLKK
                     LNSPNIGIALSYKDWPGKTELIIPIEKNILKESQRDVTLDELENEVNKIIQDKKNGSE
                     DRNKFAELRKEKIAEELKEINQKIDELENLENEIEAKQRSIVERLAELKKDPDKNKDE
                     ITKLEKEYENLEKQKNEIRKRKEDLIDREEKLTGVKSKRRIPKEIPQPKEEKKLEPKA
                     IIDPRLLEPEYSSHVVDGKLVFIKPITDIDSHCVNGIHLLDPLKEDFVYTSEYNKICG
                     KTFKVLGDNILVIGFLDKKEQIKLILLKKNDLKVVGNSDVNVYHKTPIEIRGDFLYAI
                     ENLDGKFYLTRFDLNLKSLTRSKEEVNPDSVITFYDNKIYVSGKKENGQIEFKVFKKE
                     DLSFIKKTQT"
     gene            86817..89807
                     /locus_tag="PLF66_01390"
     CDS             86817..89807
                     /locus_tag="PLF66_01390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62714.1"
                     /translation="MLQKILNILILLGLFWILSNCSRLPKDNSNLAFVSFLGKLVNVN
                     DSVISSPDSAPQVVSTDFVASINATAPTSQVVISKTQTLGVTFDKPMSASDCVATMNG
                     VAIQTTNTASSDLKTIFFKPVTGWTVDMINGVTINLSSACKSSTGSAYVPGAGVLIFV
                     AGSTIYVDGTAGSDTNPGTITSPLKTISIGVTTIASSCSVSDPCAILVKGGIYTISSS
                     ITIPSNLAIFGGYDPADWKVRRADKTSLPPYDTVLNDSSTGVSGIISNPYSTLNFSNT
                     TGINIIDGLIINGNITATASSFVSPIAITNLQTGATLSIRNSILKDVSSSPSVTSAGL
                     IAYNNSGIINVINSQVLGSTALAASERHALYYNISNTNSVITVTNSILDAGYSNINSS
                     GFFMAASSNGNINLSNNVINGAICNVCNSIGITANFATPNGMTISQNTITTRTGNNSI
                     GINFTSGTGLSILNNIITTGTATNQSIGINLVSNTSTLTASNNTLSAGSCTAAGCVAA
                     GIYSSTSLATLNVNSNTISAGSCSGSACFTYGIYLGNSATATNTTISGNTLSLGSATT
                     GGSYGMYFTSTSANQSHTISGNTFTGGTASGGQISGIYRANSSAGATVTISNNVVTTG
                     NCSGSCINRAFDIQNVSGTTTISGNTVTAGLGVGQSIGIELNNSSFTVSNNTITAGTC
                     TGGSCISRGINSGFGSPVTITGNTVTAGTCSGATCNQTGINLAIGSNPVITLSNNTVD
                     AGTPSVNTTSRLALNLTNWANGTSIQRNTFINRIGAGIPTTVRLGAMASSLTFCSNVL
                     VGGGTNSAVTATTLEIGLITGVSKFKGNTIIGASATSGTVYPVNFLTTGTYTNLALDQ
                     NIIAGDPASSAYTTGIRESGAVIYSFLSANNLSCVSTLYNENGIIRNFLCTATQIGNF
                     SSADALCSSGNLANPTGVNNTNLLPVFVNPSINDYHLNAATPVGITQAMTAAELSTFT
                     TDCGNSLDRDGNTRTANSSIGAYR"
     gene            90254..91015
                     /locus_tag="PLF66_01395"
     CDS             90254..91015
                     /locus_tag="PLF66_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002748872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HMZ62715.1"
                     /translation="MKVVVLVKQVPDTETNIKLGDKSINEAGIKWIISPYDEFAIEEG
                     IKLREKNGGEVIAVSVGPDRVQEVLRTAYAMGVDKAVHIKVDNYNTFDSLFTSQLIAN
                     FAKAESADIILAGRQSIDTDSSQVPVQIAEELGVGHVVLAIKIEINGSSAKITREVEG
                     GTATVETSLPVVVTATKGLNEPRYPSLKGIMSAKKKPIDVKQAADLGAPASKIEIVGL
                     EPPPPRIAGRKLEAADAAGFASQLVKALREEAKVI"
     gene            91026..91985
                     /locus_tag="PLF66_01400"
     CDS             91026..91985
                     /locus_tag="PLF66_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010576594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HMZ62716.1"
                     /translation="MSNVFVVGEIKDGELKKISKELTSAGRKIADSLGGQVEVLLIGA
                     GAEKFAGDLGAVGADKVLTASAGDFNAEAYANIISEVVKSKKPSVVLMPHTGYGKDYS
                     PRVAVKIGAGIVADVVGLTVDGGKVVAKKPIYSGKAYANIKVTTDIQAFTVRPNSQEV
                     GSKAGAGATEAVSANAGDVKVKITGTELTGGSKIQLTEASIIVSGGRGMKGPENFPML
                     QSLADLLGAALGASRAAVDSGWISHSHQVGQTGKTVSPNCYIACGISGSIQHMAGMGS
                     SKYIVAINKDPEAPIFKVATYGVVADLFEVVPALTSEFKKVLG"
     gene            92200..92454
                     /locus_tag="PLF66_01405"
     CDS             92200..92454
                     /locus_tag="PLF66_01405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62717.1"
                     /translation="MTSITMLDLRKNSNVLLEKLKRKEKILLSFRGKPVATIHPISQE
                     SDWESDSFLQFLNSIPKNPSKKVKTKISNDEIDSLVYGIK"
     gene            92441..92869
                     /locus_tag="PLF66_01410"
     CDS             92441..92869
                     /locus_tag="PLF66_01410"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HMZ62718.1"
                     /translation="MASNKDSKYPFWDTSGWFSLLVASESNHSKVRNISDELTKNKIP
                     FFTSDLVIQETMTLLMARKETEKAKQFWSSLVDSKVVRIERIDETRFQKSGEYFSKHI
                     DQGYSFVDASSFILMKEFKITKAVTTDKHFRKAGFIKLLA"
     gene            93192..93395
                     /locus_tag="PLF66_01415"
     CDS             93192..93395
                     /locus_tag="PLF66_01415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62719.1"
                     /translation="MKDLSYVKTKLIQEKQFIQTNPKIKDLGILGSLTHLAAKWYLTL
                     QILLFTSFVLTECALQGLFSFGS"
BASE COUNT        31235 a        16371 c        14954 g        31209 t
ORIGIN      
        1 accttccggt tgaatcaaag gtcccccgca ccagcggcaa gtctcttcta ttgttttttg
       61 aatttcaaat tcgctcataa ttgatcctct tactcttatt cattgtctgg aaactcatat
      121 tcagaaaaaa tcttaatcag tatttctcta accatcttct tagctgcaac tactgtaaga
      181 tggatatcat catctgccct cattaatgtc cgttttcccc gataaggtaa aaatttttca
      241 tattcaccat tctcatttcc caaaacattt gtcatagaaa tatatgtaaa attatacctc
      301 tcagatgctt ctttaaaaca agaattaata aaacgagttt cagaatgaaa tttagatggt
      361 cccataggtg gaagctcaat ccaatacaaa ttcatttttg ctgcataaaa cttattcact
      421 attgtggaaa ttcgttctaa atattccttt ttccattcat cggttctgaa actaacgttt
      481 cccaaatcac ttatcatcat ctggggatca tttgcaccaa ttaaaacaat acctaaatcg
      541 tatttctttt gttttaagaa ctcttccaat ctttctggcc aattataaaa ttttggataa
      601 cttaatcccg tcgaaacctt cgcaaagtta tctaagacaa tatgataatt cgcctgcaca
      661 tattcattga gaccccaaga gattgagata ccaatggaat cgccgatgac taaaatcttt
      721 aaaggattgg atctagttat ttttcctttt ttagctttta cctgatcggc ttttggtttt
      781 aactttcttt gctctaacat ccactcaatt agaattgaat ttttatatgc ctctgtccaa
      841 gcatcgtgtt tcgcatttgg aaaaatagta taccaagtat tctgattgca atattgtagt
      901 ctatcgatga catctattgt tttatctgga gttatgagag tatcccttgc tccatgaaat
      961 gcccaaacat ttgtatttct aattttacaa attctagatg tatctcccca accggaaatt
     1021 gggattagtg ctgcaaattt gttgggatac tccgttgcta acttccaaac tccatttcca
     1081 ccgagactaa ttcctgatag gtaaattcta tcttcatcta tattataatc agattcgata
     1141 tcttctataa gttttattaa atcttgtgta taccactctt cttccgattc tgatagttgt
     1201 ggtgcaatca ttataaaagg ataatcagca ttttcctctg caatatattt aggtagtgct
     1261 tgacgcttta atagattaag atcatttcct ctctctccac gcccatgaag aaataaaact
     1321 aatggcatag gttctttaga atttgtataa taggaattcg gtaagtaaat caaataaggg
     1381 atttctttgt gatgtttgga attgattgaa aaacttttta caatcaatct ttcttttgga
     1441 attggaacct gacgtgtaat acaattggaa aaaaatacaa ttgcaatgag taatactggt
     1501 aaagttttac taagattcaa actccatctt atagcatata tcaagggagg gtagatagat
     1561 agtatccgca acatcacttt ctgacaacag ccagtagatc aatttcacaa ttataaaact
     1621 catacttaat ggaagatttt ctttcaaaat ctaaatactc tgctgctctc attgtttgtt
     1681 ttgccgcatt ccccttagga acattctcta ttgattctta cgagcttgca atccttttct
     1741 aatccaccgg atcaattttg ataaatacac tcatcgacca gtcatcaaaa attcttattt
     1801 agaagtcgca ttgattaaaa ttgaaagtag aaaaattcct tgctctcaaa aacagaaaac
     1861 aaacaaaact gaatgctaaa aattatgagt agagtatatg caaaaattgg atttatattt
     1921 tttgaactaa aatattcgag atcttttttc catacttgta atccatccaa tacaatgatg
     1981 ggagaaataa aaaaaagaaa cttcaacaga aagacagaat ttatataaat tccgcttgcg
     2041 ttatctatta gccgcattaa atgagatggg ttttctactc taaataataa taatccaagc
     2101 ccaaacataa gaaaagttat aaatataaat gatatcttta tgaaaggatt taagaaaaaa
     2161 acttctactt tagaattttt caacagattg gatttatttt tcatttcagc aaacaaatta
     2221 taaaatacaa cttggagtcc gcaatatatt ccccaaatta aataaccata tgtagctcca
     2281 tgccaaagcc ctcccaaaat ccaaactatc attaagttta aattttgtct aaataaacca
     2341 aatcgatttc ctcctagagg aatataaaca taatctcgaa gccaggttga aagcgatatg
     2401 tgccatctct tccagaactc agacggattg gttgccttaa aaagcggtaa attgaaattc
     2461 aaagaaagtt caaatccaat cattctagcc atgccacgag cagcatcagt gtagccgcta
     2521 aaatctgcat atacctgaaa cgcgaatgca gctgctacaa agtagacaat tccagttgga
     2581 atattctcat ttggatctaa agcaagattt acaaagggag ccaaattgtc tgaaataaaa
     2641 acttttttaa aaattcccca aattacaaga ataagacctg actgaaattt ttcttttgta
     2701 atctgtcttt cgttttcaat ttgtggaatc agcctttctg ctctctcaat aggtccggat
     2761 aagagaagcg gaaaaaataa atcatacaca ccataggtta ataagttctt tgtagcatgt
     2821 attttgtatt tataaacgtc atagacatag ctaatattat gaaaagtata aaacgaaatt
     2881 ccaatcggca gaataatctc aatagcagat aacttgaatt ttcccccaga aaaagcaaaa
     2941 acatcattta tactttgaat cataaacaaa gtatacttaa agataaataa aataaaaaga
     3001 tttagtagaa tagtaaaagt taaaattttc tttctcttag gtaaatcgga acttttatcc
     3061 aataaaattc ccgcccaata attaaaataa atacaaaaca gcaacaggaa tgtcatctta
     3121 taattccaaa acgaatagaa tataattcct cccagaagta gtagccaatt ttgtgttttt
     3181 aaatttgatc tcttataaaa gatataaaac aggataaaga aaattaaaaa gttaacagaa
     3241 ttaaagagca taacgcataa atagagttaa gtttcttcca tcatctctac aaactttctt
     3301 ctctctctag cttgatccgg aaaagtaagc gaatatattt gaattgaaag cttaaaatct
     3361 ctagatggat tatgaaaaag gacaatttca gatttaattt tttctataaa tttttctgaa
     3421 tctgatttac tattttcttt taataaaatt gcaaacttac cctgaccaat tctagaaaca
     3481 taatcagaag attttatatt ttcaaaaatc tttttggaaa taaaatctga ataatcagaa
     3541 aagaagttaa tacctaaaat atttagaatt ctagtcacat ttagaatctt caaaacaact
     3601 aatgtaaact gtttattttt agattcactg tattttattt cacgatcaat agtttcaaga
     3661 attggattaa atggatttcg aaataaactt tctttttcat tctgcattac taagtttgca
     3721 atgataggtg caacaatatt cgacaaagat agaatcgttt tcttaaagcc tggactccaa
     3781 tcagtcaata ctttatgtat aataaataaa cccaccatac gaccaatatt aatcaaagga
     3841 ataacagtaa attctgtcat agcagcgatt tcctgttctg taataagaga ttttaattca
     3901 aagttatctt gaaaattctc tagtcgatag acatcactta cctgtgcaat cattgtgata
     3961 atcttcgaat ctttactcaa tttgaatcta tcaacagtct ctggtaataa aaaatttgag
     4021 ccaaatactt tatagtccat agtttttaca tctaaaatca taaatgtaaa attagaaacc
     4081 ttgaaatcac tcactaggtt ctcagaaata acatcataca attcgtctaa cgtctttgta
     4141 gtatatacct tatcagtaaa atcagttatg agactgcttg agtttataat cttatttaac
     4201 tcggatactt catttgctaa gttttccgaa tcgaacaatt tgcttaagaa attaccagaa
     4261 acttcaacta atattcgtaa gaattctaaa tccttttctg aataatcctc tccactaatc
     4321 atttttcccg ctacaataaa gccagaaaac tgatctaaaa ctcgaatagg tatcaataat
     4381 tcagttagag gattctccaa aaggacccgc tctctatcct ttaaattctt atctaaaaga
     4441 tctcttgcat aaagaacgtt agtctcagac tctaaaagag tatacaaagt atctcctttt
     4501 ttaataacat aatctggttc aatatcaatt ccttcatgcg caactatttt catctggcta
     4561 aaattgccat ctctagatga aaatataatc ataatttcag gagctatttg actaatgatc
     4621 gaatacatta agttttcaaa aaaatcatca aacgatttag attttaagag ctcttttgta
     4681 atctcaaata aaactaaata actttccaat ttctcttcat aagatgtttt aaattgtttt
     4741 gctgaatcaa aggcatcctc aacatttaca tcaactatag tctcagaatc tatcggtgcg
     4801 ttaggctcta tttcaacaat ttcgctatcg aaagaaggat ttggttgttc ttctgtaggc
     4861 tttgtttctt cgttaactgc ctctatttct ggtaacgctt cctcttgtgc atccctttcc
     4921 caatcttcta gaatatcatt tgattctaat tcttgactct gttcttcgtc ggttacaatt
     4981 acagtatctt catctaactc tagtgtttga gtatcaccag aaaattcatc tagcgattct
     5041 actttatttg tataatcatc aattgaatta acttctacat tttcattgga ggtaatatta
     5101 ttctcttgaa caattatctc ttcctgatct aaatcaacta cgatattatc ttcttcatta
     5161 aactctgttg gaataactgg agcagagaca acttctccct cattgaaaga gagggaatct
     5221 ggattagaat ctagatcttc tggaactaat gtatctttat tatcagataa aggtttttct
     5281 tcatctaaat catttgcaga atcttttgtt gaattgagat ttttaaaaaa gttttccgct
     5341 tcttttaaaa aactcttttt ttttttgcct tctacttgga agttttgatt tctgaaattt
     5401 ggaaagtctt gtaatagttt aagcgctttt tgcaggaaac tcattcttaa agttccaatt
     5461 cattcataat cttcttatac aattcaaagt attcagaact tgcgaaatct ttaatataag
     5521 agtcaggcaa gctaccaagt aactcatcca tataagatat tacttttctt aattcttctt
     5581 ttcttacatt tccaaaatga aaagattcag atgaatctgt tgcttctgca tcatggtcaa
     5641 gacctgtttc atctatcgtt acatcaccca gatcagataa agatgaattt tcatcgataa
     5701 caaaatcatc atcattaatg accatacctg agtctatagg attagaataa tcagcctctt
     5761 ctgtctctag attgccagtc tctaaaatat tatttaattc atccccagag agtgcaatat
     5821 catcgtccgc gactgattcc tcttcaaaga aactagatgg ggattcaaca tctaaatctt
     5881 caggaacatc ggacagactg aaattgtctt cactttctgt tgttcccaaa tcagaattgt
     5941 ccacttcttc tacttcatag ttttcatctg ctgttatatt tcctaactca tcagctgata
     6001 aagaaattga gtcgtcttct tcattggatt cttcaaacaa agaatttcca gatgtgtcaa
     6061 cctcttcgga ttcgatttca ccactttcta agatattatt taattcatca cctgataaag
     6121 aaatttcacc ttcatcttca tttgatgatt caaataaatc agattgcgat tcctcgattg
     6181 caaattcatc agaagcatct gtcaattcaa cagtatcatc ggattcatta cttatctcat
     6241 tagagttatc tatttcttct acttcatagt tttcatctgc tgttatattt cctaactcat
     6301 caggtgataa agaaattgag tcgtcttccg cctcgttaga ttcttcaaag aaagaattgc
     6361 cggatgtatc tacctcttca gattcgattt caccgctttc taagatatta tttaattcat
     6421 cacctgataa agaaatttca ccttcatctt catttgatga ttcaaataaa tcagaacttg
     6481 tctcatcaat gtttaattca tcagaaactt cagataaatc aaaattatca tctgtgttta
     6541 cagagtcgtt aggcgattcc gcatcttcaa cttcaaaatt ttcttctgca gtaatatttc
     6601 caagctcatc aggagaaagt gatatagatt catcttcctc tatgctttct tccccgtcat
     6661 ccgtgtcaaa aaaggatttt gcattatcaa tatcaaaatc ctcttcctct tcttcgacag
     6721 aagcctctcc cgattctaag atattattta gttcatcccc agataatgcg atactctcct
     6781 cttcatctgg agtatcaaaa gaaatatttt gttcctgttg atccggatca aaatcttctg
     6841 tttcaatacc atcttcctcg gaatattctg aatctgtaat tctactcaat tcttcaggcg
     6901 atagagcaat ttcctcttcg ctttcaccac tctcatctgt atcaaaaaag gattttattt
     6961 ctgattcatc agagatctca tcttcgctcg atacttcacc tgactctaaa atattattta
     7021 gttcatcgcc agagagtgca atatcatcct cgttttcaga gtattcaaat aaatcggatg
     7081 ggggatcaga aataatattt gctgtttcat ctaacgttga atcagtttct tctaaacttg
     7141 taatgttatt tagttcatct ggagacagag caatatcatc atcttcgttc gcagagtctt
     7201 ctgtatcaaa gaatgatttt gtgacaaatt cattttcttc tgaatcctca tcttcatcag
     7261 gatttaaaat ttgatctaat tcatcggtag ctaaggttat attgtcttca tcatcttctt
     7321 caataaatgt atcatagcga tcttcaaact gatctccttc atgtgaatcc tcagaaccgc
     7381 caacaatatt atttaattca gactctgata atgcgatatc agtatcatcg ctcgtatctc
     7441 taagtaatat atcatctagc tcatcctcag aaaaaggttt agatcctaat tcatcagctt
     7501 caacttcttt taaagaactt aaattaaagg gaattggttt aggggaatcg gaacttggat
     7561 cagtaaaatg agaagtataa cccgaggaga taacgttagt tccctcattc tcctcatctg
     7621 tcatgtaggc aatatcatca acgtaaacat caaaatcaag agactcttct aaatcagcaa
     7681 atctttcatc tacaacacta ccaacagaac gatcagtttt taaatcatcg tttgctaatt
     7741 ctgactgttt actttcttcc gccatctttt attccttact cgattctgat aacctgaccg
     7801 tcttttactt cagtatctag atttaaattg gaattgtcaa cgttattggt aaatccaccc
     7861 gcagtatcaa ctaaatcttt taattggctg ccctgaatca tctcatagat ccctggattt
     7921 tgaactttcc caataatttt aacatgaatt tttgtaggag cataaattcc tttcagccat
     7981 actctatttt gcctcaagaa tattccaaaa actaaaacaa gcaaggcaaa taataggatt
     8041 agaaacttat tttttataaa aactcaccta ttcctaactt caattttcgg ctaatagaaa
     8101 gattctatat accttttatc aaaatttata atttgataaa tttttctata attaattcga
     8161 tttcgctttc tctagccgtt agaaaatgaa atatatttca ctaatctagt tattggcaac
     8221 taacttttat gaattaatac cctagatttc gcttttttta tatcaaggtc tcttgttttg
     8281 gaagaagatt cgattgcctt ttctaagtcg gcttcagatt ttactggatc aatattctct
     8341 tttaaatcac caccatttgt tagaacttta atttggttgg atttaacttc ggcaaaacct
     8401 ccatccacaa ctattttctg gatcttgcca tctagtttaa cttctaatat gccatactct
     8461 aattcagaaa caatcggagt gtgattgata agaactccaa aatagcccat agcaccagga
     8521 agatttacat aatctgcctt tccagagaag atttccttct ctggagagat aacggataga
     8581 gttaattttg aatcggacat cttaagcctt taatttctta gctttctcaa ctacttcatc
     8641 aatagaacca accatataaa acgcttgttc tggtaggtca tcatatttac cactgattaa
     8701 ttctttaaaa gaacgaaccg tatccgctaa tttaacatag cgacctggtg atccggtgaa
     8761 tgcttccgca acgaagaatg gctgtgaaag gaatttctca attttacgtg cacgagcaac
     8821 caagatttta tcatcttcag aaagctcatc cataccaaga attgcaataa tatcttgtaa
     8881 gtccttgtat ctttgtagaa tacgttgcac ttctcgagct actgtataat gatcttcccc
     8941 taatacttca gcatttaata ctctagaagt tgaatccaaa ggatccaccg ccggataaat
     9001 acccttagaa gcgatattac gagaaagaac cgttgtagcg tccaagtgag taaacgcagt
     9061 agcaggagct gggtcagtta aatcatccgc aggaacgtaa atagcctgaa cggaagtaat
     9121 cgatccatgt tgtgttgatg taatacgctc ttgtaatcca cccatttctg tagaaagtgt
     9181 aggttggtaa cccacagcag aaggcatacg acctagaaga gcagatactt cagatccagc
     9241 ttgcgagaat cggaagatat tatctacgaa taataagata tcagcgttag cagaatctct
     9301 aaaatgttct gccattgtta atgcagagag agcaacgcga agacgagctc caggaggctc
     9361 attcatctgg ccatatacta gaacagtttt atctataacg ccagattctt tcatttctcg
     9421 ccaaaggtca tttccttcac gagttctttc tcccacacct gcgaatactg agtaaccacc
     9481 atgctgtttt gcgatattgt tgattaattc ttggattaat acagtctttc ctaccccagc
     9541 accaccaaat agacctgttt ttcctccctt aatgtaagga gcaagaagat cgatcacctt
     9601 aatacctgtt tcaaagactt ctgtcttagg ttttaagtct tcatattttg gagaagccat
     9661 atgaatagaa cgcttctctt ttgcagtgat tggagcacct tcatcaattg tctctcctaa
     9721 cacattgaaa atacgaccta gagttacttc acctactgga acactgattg aagcgcctgt
     9781 atctttcaca gctaaacctc taaccatacc atctgttgag gataatgcaa tcgctcttac
     9841 agtattatct ccaagatgtt gttgcacttc tgcaataact ctctcttttg ctccattttt
     9901 ttctacatca atttctaatg cattgaaaat ttcaggtaat tgaccattct caaaggatat
     9961 atctaaaacc gaaccaataa cttgtttgat ttttcctaca ttcgcactca ttcataaact
    10021 cccttaaact ataactctat tttagtgaat ctgcaccagc aacaatctct gaaatctctt
    10081 gtgtaatttt ggattgtctc actctgttgt atccacgaac taaaactttt atcatttctg
    10141 atgctgcatc ggttgctgat ttcatagcaa tacgtcttgc tatttgttca gagcaaaccg
    10201 cttctagtat tattttatac aatactgttt tgattaccaa tggtaagaga gaatttaata
    10261 tactctctgg actcggctcg taaatgatag attcacttac ttcttgcgcg tcacttggtg
    10321 gctcaattgg taatactctt gtaatctccg atctttgatc cgcagaagaa tgatagactg
    10381 tagaaattac ttctaaccca tcaattctct tgtttgaaaa ctcttccata aataagtttg
    10441 caaaaaattc tgcttcttta taaccagaat tatcatctat attgataaat gattgtttga
    10501 ttggttcttt tgcaaaacga aagtaagaga tccctttctt tccaataaca tataaatcat
    10561 aggcaatgcc tttcgatttt aattctgcaa caaaagaacg agtcatacgc aatatgttcc
    10621 cgttataacc accacagagt ccccgattag cagtcactac caatatagca attttcttag
    10681 gattttcctc tcttcttaag agaggacttt ccactttgct tgccaatgat gatagagaac
    10741 caaccaactc tttaatttta tctgaaaatg gtttcgaagc attcactcga ttactcatct
    10801 ttttggattt ggcagttgca accatttcca tagtccgagt aatctttttt gtgtttgaaa
    10861 cagaaactat tcttttttta atttctcttg gtgaagccaa tttgtagacc ctttattttt
    10921 ttaagaagtc agctactact tctttaattc tttcagaaag aacattttca tctttgattt
    10981 tcttctcatc tcgaatttca tttaataatt caggatgatg tgtcttaaca tgatttaata
    11041 aatatgtttc aaatgctttt acttttgcta ctgggattgt atccattaaa ccgcgtgtta
    11101 ccgcaaagat tactaatact tgatcttcta caggataagg atttgattgt ggttgtttaa
    11161 gtacttctac aattctattt cccctatcta actgcgcttg tgttgtagca tctaattcag
    11221 tccctaaaga agcaaaagct tccaattctc taaactgagc taaatctaat ttcaatgttc
    11281 cggcaacagt tttcatcgcc ttgatttgag ctgccccacc cacgcgagaa actgaaatac
    11341 caacatccac tgcaggacga ataccagatg caaatagatt tgattgtaag tatatctgtc
    11401 catctgtaat cgaaataacg ttagtaggaa tatacgcaga tacttcccct tcctgtgttt
    11461 caattactgg aagcgctgtt aatgaaccac caccatattt ttgatctaac ttggcagctc
    11521 tttctaataa acgagagtgt aagtaaaaca cgtctccagg atatgcctca cgacctggag
    11581 gtctacggag cagtaaactc atttgacggt aagcgacagc ctgttttgtt aaatcatcat
    11641 aaacgattaa acaagctttt ccttgatcat acataaaata ttcagccatt gtacagccag
    11701 catacggagc aataaattgt aatggagctg gatcagaagc acctgcagct actacgattg
    11761 tataagccat tgcaccctta tcttgtaact tttgcacaac gcctgcaacg gtcgaagctt
    11821 tttgaccgat agcaacatat acacaaataa ctccggaacc tttctgatta ataattgtat
    11881 caattgcgat tgtagttttt ccagttccac ggtctccaat gattaattct cgttgacctc
    11941 ttccaattgg gatcatagag tcaattgcct taactcctgt ttgaagaggc tcacttaccg
    12001 gataacgctt agaaatacca ggagctaaaa tctctactgg tcttacttta tcagtatgaa
    12061 taggaccttt tccatcaatt ggctcaccaa gtgggtttac tactctacca agtaatccct
    12121 ctccgacagg cacagagaaa atggatccag ttcttttcac tgtaaatcct tcttcaatat
    12181 gcttgtattc accaagaata acaaccccaa cagaattctc ttccaagttg aatgcttgac
    12241 cgcgaacacc gttctgaaat tcgagtagtt ctcctgccat aacgtttttc aatccgaaaa
    12301 cacgcgcaat accgtccccg atttcaataa ctgtaccaac ttcgtctatc gctaaatctt
    12361 gcttgtagct ttggatttct tttttaatta aggatgtaat ttcctcagtt ttaattttca
    12421 tagtatgccc cgcctagttt gctctggagt aaatttttct ttatattcgt cagttgactc
    12481 ttcattgagc catcaattaa attatcattg aagcggataa ccattccgcc aattaattca
    12541 ggtttaataa cattttcgat aatacattca ccattgtatt tttgagataa agcagagcgt
    12601 atctcactca cttgagaatc tgatagttta gatgcggact ctacataagc acgaatcctt
    12661 ccatttaatc gatcattacc aagggagaat tgatgtgcga tctctcttat aaaaaagata
    12721 cgatcttttc ttaataaaag ataaagcaaa gagttcacag tctcagatag actattttta
    12781 aacgaagatt caattacttt tatcttctgt tcttttgaaa tcttagggga aagaaaaaac
    12841 tcccacacct gcttatcatc gcatatcgct tttactgctg cttgtagttc ttcttctatt
    12901 gctacagaat ttttcttttc cgcagctatt tcaagaagag cttccgcata aactttacca
    12961 acgtgaatat tatccataga ttatactttt aaatttttga gttttgcgat ttcttctttc
    13021 acaaactcag catgatcttc cgatcgaacc tttttctgta gaatttgacc cgcaatcatt
    13081 acagataaat caaccacttg tgcttgcagt tcttgaacgg cttttgcctt tgcaagctca
    13141 atgtctttca gggactgttc ttttaaagct ttaatctcat cctgagtctc ttggacagtc
    13201 ttggatttga gctttacagc atcagcattt gcttcgttta caattgctaa tgcttcatct
    13261 cttgcgccat caagctttgc tttataagtg gctaataatt cttctgcctc tttgcgaatc
    13321 ttatctgcct tgtcaatatc tccgtgaatc ttctcagctc tttcatccaa tgcatgaaga
    13381 attggattcc aagcaaattt tcgaagaata aaaacaacaa ctaagaatgt aacaatggtc
    13441 caaactaata gaccaggatt tgcatcaagt aatccgcctg ccgctagata aatcaaaatg
    13501 atttcctctt acttatttag gttgttcaac tgcagcaggt gtgtgtgctt ttaaagaatt
    13561 tggcaattct ttgtttaaaa caccgccagc ttggaatgca atcacgagag caaataacgc
    13621 agcaccttca atgaaagcag cagagatcaa cattgctgtt tgaattttac ctgcagcttc
    13681 tggttgacgg ctcatacctt caacagcttg accaccgata cgtccaatac ctaaaccagc
    13741 acctagaatt gcaagacctg ctccgattcc aacaccgata tatcctaaac caaaatccat
    13801 aaaaatctcc tcttaaatca aataataaat taaaataaaa acctagtgtc tatgcattga
    13861 cgatccaaca aataaagatg tcaaaagcgt aaaaatgaat gcttgaacaa aggctacaaa
    13921 tatctccaat agataaattg ctgtagctcc accaatcgaa attggaacaa ttgcatacga
    13981 tctaaactca aaaataaacc ctaataatgc caatattaca acgtgtccag ctgtcatgtt
    14041 cgctaaaagt cgcacagtga gagcaaaagc ttttgcgata ggtgaaataa taaactctag
    14101 aggccacaac aaaggccaca atggccaagg aacaccatta ggaacagagt ttggaatgaa
    14161 tgcaattccc tgataagaaa aaccagttcc atatatcaat aatagagtaa tacctgctaa
    14221 agtaacagta acggaaacat ccccagttac agtaattcca ctccatattt ttgttaatat
    14281 agaatgatgt ggatcttgtc cagtcgcata ggctaaaatc tctccaacag atgggattaa
    14341 accgaacaag ttacagaata atataaagaa aaatagagaa aataaataat gataataaga
    14401 atgtccatga ccatgcatgt tagggtctat cacttctttt cttaaaaact ctaatacagt
    14461 ttcaatcgcg cctgccatcc ttgactcaat tcgaagagga tttctcgcaa tcactctagc
    14521 aactggaata aatacagcta ataagaaaaa ggatacaatc cacatcatcg taacacgacg
    14581 tgtaatatgc atatcaaagc cgccaacaaa gtggtatttc tttccatgac taccatcaga
    14641 gtgctcttca aatatcgcag gattgttttt gtcaaaaagg ttatcacctt catagacctt
    14701 gacaccacca aagtttaaag gaaactctgc atgatcaaag aggtggtgat ctatgatact
    14761 aggcaaatca aaagcttccg gctcatgacc ttcactcgca aaggaggaaa atgttagaaa
    14821 aagggaaagt aaaataaata taactttgtt aatcatcttt ttttgtagaa cgattggtga
    14881 taagggcaat atttaaaata tttagattat gcgcaataaa aaaaccaaaa accaaactaa
    14941 aattatttag atacgttact aaaataaata aaataattac atttattaag atggaaaaga
    15001 atgaaataaa tatttgcaaa ttcccagcta acttcaaatt cttttgcata ataacaattc
    15061 tcaaattcag taaaagaatt tgaatcaata aaccaataaa tacccctaga gtaaaaacaa
    15121 gatcaggatt tgaaaagtat cttgcctcaa tacgagaaga gataaaaaga tagattgcgg
    15181 ataacgataa gtaacttaga ttaaacttaa ttttgtctac ttttgacaaa tcaaaggctt
    15241 gcatcattca agtcttttat ctaaataact agaaacaaga ttttttttaa atgaaattag
    15301 atttataaat aacttaaacc aagaatagac cgcaaaataa atagatttca ttttttgtaa
    15361 acctagacac tctaagttta ttagaaaatc ggcttcacgt ctgaaaggaa ctgactttag
    15421 attccaaaaa gtagaagaat acaattttga gaaaaatatc ttctcaatca acaaaaaaga
    15481 tcacaaagac aatcctagag attatcaaaa tcgacctatt tttaattctt atatttttca
    15541 cctaacatta tctttacggg agaaagctca atcactcaac ttaaatgaaa ccgacattta
    15601 aaaaatttta ttcccattat taataaaaat aggaaagggc ttgagtaggt cagaaatgat
    15661 agagttttct tagagaggat aaaatgctaa aatatttttt ctacacactt atcctcaaat
    15721 agaacaggaa gttggtttag tatcaaatta ggttgcctca gaaatctgat cctaaatcaa
    15781 taaattaaac caaccacaaa aaattaatct actttagact taaaaagagg actataaatt
    15841 tctttatatt gataggctaa aagagaatta gcaatgataa gaaaaattgc tacaggcaaa
    15901 ccctctggag ccaaaaatat gtgaacaaag aatatattca cgattatagg tgatatgaga
    15961 actaacgcta agggaacaaa gcgattggat aacaaagcca aaccgcagat taattcaact
    16021 acctttactg taggcattaa atatacagat gcatttaatc catcattaaa gactttcatt
    16081 gcgcctgtca tttcgggcgg cacaataagt ttaaataaaa aagtaacgga tgaaaacaaa
    16141 aataatgctc ccatcaaaat tctaattatc aataaaaaaa ttttcataaa gattttcctt
    16201 catttatttt aatataatcc agcaactgat ctagagcaat tccccaacct gaataaaaac
    16261 ccatttcaga gtgttgttta caattctcta aggtccaatg ccttgctctt gcagtatatt
    16321 ttgtctttcc atttgataaa ctttccagct cgataatacc tgtaaaaaaa ggatttttac
    16381 tcggaatcca accggctgta tacgcatccg taaaaacaaa tttttgattc ggtattattt
    16441 ctaaacagat tccatgatta ggatgctctt ctccgtctgg tgatttcatc agagtataaa
    16501 actcaccgcc ggctctcaga tcaattctac agtcagttac gagccatggc ttaggacaaa
    16561 accatggctt gagatgttct gaatttgtcc aacatttcca aatcacctcc gctggggcta
    16621 atacaattct ctctaaaact aattctcttt catcgaggct actcattgtt aaccaccttt
    16681 ttatttatga atataattta aataactttc cgaaataacc tctattaata atacattaga
    16741 aggattaaat tccttaccat aagtcataat tagaaatcta ctgtggcaat ttcagatcaa
    16801 aagaaagcct tattgctgtt aaaagcgtag tcacaaaaca tctatttgcc ccaatttcat
    16861 ttcgattggg gttaacccta aactatttaa acctaattta ctcaactgtc taaaatataa
    16921 aaactgaatc gcactttgtt tgttaaatag aaaaaaacat cttgtgtgta ggcttgttta
    16981 attttccata taaagaaaat aacaataaca tcactccaaa acatcatcaa tgataagaat
    17041 taaataaacg ggattctcgg aaaataattt gaattttttg aacttaaaaa aagagtatat
    17101 cctatggaat caaatcaaga attcatttat ataatcttta tttttttaat ctgtattgct
    17161 agcttttccg gtctttggct aacaatactt ttcctcacat cttactttgg aacttggagt
    17221 aaactttaca atcgttataa tttcccaaaa acccttagcc agcctctatt aataaaaaaa
    17281 tttcaatctg ctcaaattgg aatctccaat tacaacgccg ttcttactct aacattttac
    17341 aaagagggaa tttcatttga accaatattc ctttttaaat tcgcacatcc caaatttttg
    17401 attccttgga aagatattaa gctaaataaa aggagccaat ctattttttc ttggaataaa
    17461 ctagatatag gaaatccacc atttgcaaaa ataaatgtaa gtgataattt attagaaaca
    17521 attctcgcct attcagaaaa agaagaaact gacgataggt ggtagattaa attagagttt
    17581 gtaaatgatc caatatttcg ccgcttaaag ctggatttgc agctattgct ttattaaatg
    17641 ccttaatcgc cgcttgctta tcacctctct tatgaagaag aactccaaaa gagtctaaat
    17701 aagctgcatt gttaggattt tgtttaaccg cttgttttaa atagtcagta gcctccgcaa
    17761 tctcttctgc tgtaggattt ttttttgtag caattaagta acctgcagag tttaaactat
    17821 ttgtatattc aggatttagc tttaaaatat ttcgatggat ttgaattgct tcatctagcc
    17881 tatttaattt ttccataata tgagctctaa aagagagtaa ctttaaatct ccaatattga
    17941 ctcttaatct ctcatcggtt agatctaaag cctctttgta ttcctgtaac tgaattaaag
    18001 aaaaaataat caatcgaaac acattatctc tttctatgaa ttttccaact ttatctagaa
    18061 gagtttttaa aatagtgata cattttttat gatttcctgt gcgggaacag caaatagcaa
    18121 aattataaag aagttctgga tcctcaggtc tctcaattaa ctctttatca atcatggtca
    18181 atgcaagagc aaaattcccc ttttcaatct cttttaaata cttcttttct ttcatttaaa
    18241 cgcctgctat tcttggtttt ccatccatca aactgggtat attttgtgtt aaatattgct
    18301 ctttttttaa aattcattga cccaatctgt ttctttgatg gaatgctagt atatatttcg
    18361 gataaaagca aaaagttcaa gaattttcta ttatgaatat tttaataaca ggtggatcag
    18421 gtgtaatcgg cactcgcttt attagtaagg tcctgcacaa acataaactg attgcaatcg
    18481 gtaagaattt ttcaaaattt ccagaattta ttcgattaca taaaaatttc aaattttacg
    18541 aatacgattt aacttctgaa aagaaattta atataccaga taagattaac tgcatcctac
    18601 atcttgcagg cgcagtctcc ggatcaactc acaatactga agattatatg agaacaaatg
    18661 tattaggaac aaataaactt ataatctttg caaaagaaaa taaaattcct aattttatac
    18721 ttgcaagttc tgtctctgtc tatggtcatc agccaggcaa aaaacttgtc gagacagatt
    18781 tacttgcggg taatacagat tatgcgatat caaagataga cgcggaaaaa ttagttcaaa
    18841 tgtccggatt ggagaattat acgattttta gaattgcttc cgtattcggg aaagatacaa
    18901 aaggctttat aacaaaacta gaacgaataa taaaaaaaag aatacttcct tttcctgaag
    18961 acaggaactc aaagaaatca ttcattcata ttttagattt agtagagttt ttagaaagag
    19021 ccattgaaaa tcctaaaaag ggaattttta atttagccca ttcagattct ttttcttttt
    19081 cggaaatttc agttatttta aaagctaaaa caaaatcaaa ttttgttatt aaagttttca
    19141 tagggaaaat acttttatcc tttatccata aaacaaatca gtttctttat tttctaaaaa
    19201 ttacgaagaa tccaaaaaga attgatctta gaccacttct agaatttata gaggtgaatc
    19261 caaaaaaagc aattcaacaa tttgcatact tacctaaaat acatttaaat agcgattggg
    19321 agcatttatg aattccacat tagaacataa aaataaaatc atttccatac ttggaaaaga
    19381 aaaagtcttc tttcgagaag atggagaaat ggatgcagga acttttgatt cctatggaac
    19441 tgatagaacc aaagttcata aaccaaatta tgacttacta actttcccaa aaacaacaga
    19501 agaagttgct gctcttgtaa aatatgccta tgaaaataat attaagatcg ttccctccgg
    19561 tggacgaact ggctatgcgg gaggagcaat agcaaaagac aaagaacttg ttatttctct
    19621 ttccaaaatg gatagtgtta ttgattttga tccatttttt gcttctctca cagtgggtgg
    19681 aggcatgata actcaaaatg tccataaaga agccgaagaa agaggttata tttttcctgt
    19741 agattttgca gcaacaggat cttctcatat agctggaaat attgcaacta acgctggtgg
    19801 agttagagtt gttcactacg gcttaatccg aaattgggta ttaggaatga ctgttgtaac
    19861 aggaaaaggc gaagttctaa aattcaatgg gtctatttta aaaaataata caggctacga
    19921 tttaaaacat ttatttattg gatcagaggg aacgttagga attataacag aagttacatt
    19981 aaaattaact aaaaaatcta ttgatacgag cgtaatcctt ttagcagttc cagattatca
    20041 atctatactg aatgttttca aagaaacaca tagctctgtt ctacctctac ttgcatttga
    20101 attcttttct gacttatgtt gtgaaaaagt aaagcatcac ttaagtctag gagatccatt
    20161 tacagaaaaa agtccatatt atgttttgct tgaatttgaa attgcagaag agtcagataa
    20221 cgataaatta tattcatttt tagagcaaat aacagagaaa ggcttaatca atgatggaag
    20281 cattgctcaa aactcccgtc aaaagcaaac attttggaaa tatagagaag ggatatccga
    20341 aagtctttcc ttacaacata cagttcataa aaacgatata tccttaccac ttcgcaatat
    20401 ggaacaattt atttctgata tggaaagtct tttagcaaca aaatacccgg gattcgaaat
    20461 tgcacttttt ggtcatatag gagatggaaa tttacatctc aatattctaa aaccagccaa
    20521 tctttctgat tcagactttt ttgctgcatg taaaaaagta gatccagata tgttttcatt
    20581 gattcaaaaa cacaatggat ccatcagtgc agagcatgga attggtttat taaagaagga
    20641 ctttttgtct ttttcgagat cagcggaaga aataagatta atgaaagaga ttaaaaatgt
    20701 atttgatccg aaaggaatca tgaatcctgg aaaagtttta taaaacaaat ccgatgttta
    20761 aatagatgaa atactttctc ttctttttat taataacact ctcggtcatt ggtcaagaaa
    20821 aaccaacaga ccaagagttg attgaaaaat tagacaggac ctttctaata aaagaaggtc
    20881 tcacaaaagg aagactctac ataaaaaaag gaaaccatga gtcccattct tggaacgtta
    20941 gtatatttaa agaaggggaa gatattttat acatctttac tgtaacgcat tttaaaccag
    21001 ttgcaaaact actttcgatc aaaagaggtc aaaaactaat ttattcgaat gttctctctg
    21061 gtaaaatttt ccaaatagaa caattacaaa gattagaaaa tattttagga acatccttta
    21121 gctatatcga cttaagcaat tatcaatacg aatcaaatta caaaccattt aaaccagaga
    21181 actcagaaaa agaaaatcaa gaaaaagaca gactttcaat tcatatgata ccaatctccc
    21241 attatctata taaagacatt gaaatagata tggctaatac ggattattcc cttcgtaaat
    21301 taacttttac ttctccaaat ggcatcattg aaaaaacgtt aaaagtaaaa tatggaaaaa
    21361 taaaacagat agatggtaaa gctaaatcag aaataatcga tttaaaattc atagaaataa
    21421 ctaataatgc tacaagtgaa atttcgattt tagaatttca agaatggtat aaagatacta
    21481 agccagataa aatattctat gaaatgaaat ctctctatga aaagtaaaat ctctttaaac
    21541 aaaatcagaa tgctataata aatagtattt aaatctattt ttgaaataat caaatatcta
    21601 aataatagac tctcttccct cttaaacgaa tgtattgttt tatttcctga acgggttctg
    21661 gaagttcgat aagttttgcc tgcaagaaat cataagttaa atcagtaata atttccattt
    21721 gatcaaatcg aaaacattta taatcatttt tagtttttag gattagaata ttacaataat
    21781 ctaatttagt ttctttgcca tctgtcttca aaccagtgta agcaggaaat aacggataac
    21841 gtttattttt tattctaata aatgttttgg tagcatctac atcttgtaat actttttttt
    21901 catattcctt tactaaaaaa cttaaagatt tatatgtaag caaaaaaaat cgttctccaa
    21961 attcaatatc taacttttgc gaagagcgaa caattctttc agatattttc tcaattcgat
    22021 ttcgaatacc ttcattcaaa aactcaatgc tatcatcttc tttgattttc tctagagttt
    22081 taaaaattct atcaaactca atctttcggt aagttctctt ctcgtgaact ctctttaata
    22141 atttatctaa agtgactagc cttaatctga aatattcaga attagttttt tctaccaata
    22201 cttgtaattg tttaaacaat ttatcgattt tatctagatc atctttctct gagtaaatct
    22261 ttctctcgta gttttctatc tctgttacaa gttggttttt tctgtcaaaa aaatctttaa
    22321 catctgcatg gatattatat ctactcaaca taaaaaaacc aatttaattc gcatgcttcc
    22381 ttagttttat agataaaata attcctactg caaccattga catgagcaag tgagaaccac
    22441 cataactcat aaatgatagt ggtaaacccg taatcggcat cagaccgata acaattccaa
    22501 tgttaatagc catatgaaaa aatattaacg caacaattcc cgcggccaga agggaaccaa
    22561 accgatcttt actttcaaaa ctaatttgga gacctctcag cggaattgcc aaaagaaaaa
    22621 ataacaataa tacagaacca ataaatccag tctgctccgc ccaagatgca aaaataaaat
    22681 cagtgctact ttctggaacg tgcggaattc ttccttcggt catttctcca tgaccaaatc
    22741 ctttcccaac gaatcgacct gaaccaattg caggttttga tgcacgtaat tgataacctg
    22801 caccttgttt gaattgatca ggatttataa atgcagtcaa acgaatgact tggttttctc
    22861 taaatggaat ttttttagaa actgcaaaag caaataaaaa gctaatccct aaaaccccaa
    22921 gtggaatgta atactttctt agattctcag atccgcgagt aattcgaagt gcaatcaaaa
    22981 ctaaacttat gaaaattaaa gttccaccaa acataatcat aaaattttca tttgagaaaa
    23041 ttttaaataa gaaactagta ttttcttcaa ttacttgatc tacagcatcc tgtaaaattt
    23101 taaaattctt tggattaatc aaaccagaca cttctttggc ttgtatagtt tctccattca
    23161 agacaggcca gatttttcca cctaattgat ttacaatcga taggacttct gttttctccg
    23221 ttttagttag caactcaacc agaggcgcca ttaacgttag acgtgtatat tccaaataca
    23281 taggaatact gagtgatatt ccacctaata taactaagga actaatatgc attaagtcag
    23341 ctccacctag aaacaacata gctaataaga ttggaataaa agatacagca gttccgaaat
    23401 caggctgaac aataataaaa accattggga taataacgat aataaaagga attactagaa
    23461 ctgtaatatt tcgtatttct ctttcacgca aaaccagata ttgacccaac aaaataaccg
    23521 tcgataactt tgcaaactca ctcgtctgaa aactaaaagg accaagcttt aaccaagaac
    23581 gagcacctcg accgttaggc aaataaccga tccccggaat gagcgttagg atcaatagaa
    23641 aaattgaaaa aagataaatt ggaagagcat aagatccaag taattggtaa tttagacggc
    23701 tcataaaata cattgtagtc aaaccaacta taaaatagaa aagttgttta taccatttac
    23761 caggaccatc tgtaatattg gattcttgaa agtataaagt tatgatacca cataaaacca
    23821 caacaacaac ggatataatt agaaaataat caattttctc taatctacgt tctgccatta
    23881 ttgaatctcc tctataggtg tttcatcatc cattctatat ccatcagttc gatctgttct
    23941 cctaaaagag ccaggaggaa aagctgcttt aaaaacttcc ttagcgatag gagcagctcc
    24001 aacggatcca ccaacaccgt attcaacgaa tacagttact aacacctgtt gctctacagg
    24061 tgcgttgtat ggaccatacc caataaacca agcatgatta gacttagaca aacctcttct
    24121 tcgagtttgt gccgttcctg tctttcctgc aatctctggt agatctggca aatttaaaac
    24181 tctagatgca gttcctgttt ttacaacggc tctgagacct tgtttaagcg cttccaaagt
    24241 ggaacttttt aatggaatat ctcttaacat cttccctgga tttttaaaaa caattgaatt
    24301 atcaataggg tttctgattt cagaaactac atagggttga aatattttac cattattaag
    24361 tagtgccata taaaaaagcg tcatgcccat aggtgttgta gaaataaaac cctgtccgat
    24421 agatagattc acagtatcac cagcaaacca tttagttcca taggctcgct ttttccattc
    24481 attactagga acgaatcctt cgatttcacc aggcaaatct atattacttt ttttatctaa
    24541 accaaataac ttcgcataat atagaatagc ctcagatcct aacttatgac ctaaattata
    24601 aaaatataca gaacaagatt tttcaattgc atgcattaga tcatttgttc catgcccctt
    24661 cttgtcccaa catttaaact cttgatctgg gacaccagcg agagttgact ctaaaataaa
    24721 acttccattg caatgaaagg tttgggaagg actaaaatct atcttatgct cactctctaa
    24781 tgcagctagt ccaactaacg ttttaaatgt agatgctgga ggaaatttag aacgtagagc
    24841 aatgttcaaa aaaccaccat tatttttaac tcggtttaaa tgttgtaact taagctgttt
    24901 atttttgcca gaaagtaaat ttggatcaaa gctgggatta gaggtcaaag ctaaaatttc
    24961 accggttgct ggtttaatcg caatcacagt tccacgatag ttttttagtg cgttatatgc
    25021 agcaaattga atttttttat caattgtcaa aactagatgg tttcccattt tagcatgttc
    25081 aataatcctt tcttcttcaa tgtttccttc tgaatttctt ttttgataac ggaatccatc
    25141 tgttccacga agataagagt catactgaat ttctagacca tcctttccaa ctaattgata
    25201 ggatttgatt tctctgtttg ttaaatcttt taaatttgga agaccaacat acccagaaac
    25261 atgagccagg gcaggtccca taatataatt tctttttggg gaaggaacta gtataacata
    25321 tttcgcaata ttatcaaaga cagaaatccg ttcatgttgt tctgatgaaa ttccttgtaa
    25381 taatacaatg ggaatttttg atcttacttt ttttgtaaac ttaggttctt taatttcgtc
    25441 tgcgtaataa gcttctggta tagaaagagt tttataaaat aaaagcataa actctttaat
    25501 ccgctctgga ttatttcgaa gaagtccagt atttacaact acatccaacg aggcggaatt
    25561 cgaaattaat acatgctgag tagacatttc aggattgata aaatttctat cataaacctg
    25621 acctctagcc gccggtaaag actcacttct tctaacaaat cgttctgctt tgagtgagtt
    25681 ttcacttcca tttataattt gtaggttaaa tagttgaagt ataaaagcaa tcagtgtaaa
    25741 tataatgaga cctgtaaaaa aatacaacct atagcgaaaa ctttgttcta atttgaactc
    25801 tgttgatgaa gttgtatttc ttgccattat cgaagaccct ctgaggaagt aggatctaat
    25861 ttataaatcc aagataagat aaaaaacgtt agtggagcaa tagcagcatt ataaactgac
    25921 gttgctataa aagagtagtt taaattagaa tgaaagaaaa acgaaaataa catataagtg
    25981 aaaattcgag ccaccaaagt caaggcaaaa gcgaaaatag atattgatat aaagttttca
    26041 ttataagaca tacgtccaaa ttttcccaac aaataaccta ccagagagaa acttagagaa
    26101 tgtattccta ttttataaaa aactttccca cctaaacctt cttctccgcc taacgctgca
    26161 tccgtcagca atcccccaat aaaaccaatc caaagaccac tcattcctcc ctttcgaagg
    26221 gcaaaaaaaa ttacgagaag aatcatgaaa tcaggcttaa tagcagtgcc tatatcaaat
    26281 aaatttgtcc catttaatat atgagccagt agaataccta cgccaattac tatacgctct
    26341 aatatcatgg gtttgactcc tctaattttt taaccatttc atcatcatca ctatcagatg
    26401 atattttttt cttttccttt ggctgcggat tatttggatt aaccgttttg gcttctattt
    26461 ttttgggtgt tggctgaatt ggagctgggt tggttttttt atcttttaca tcttttcctt
    26521 caataatctc gcccgggaaa tttaactccc caaaatatgg attttctatg gatatactct
    26581 tttcttcagg ccaatcttcc acccatttgt caggagtctt tttaataaca gttacaaact
    26641 gtaattcctc aaaacgaatg taaggcttga tataagcagt tttaaaagaa ccggatctag
    26701 gtccttcttc tgttataatt ccgacaggaa tagccgcagg gaacaagcct ccacctgagg
    26761 aagaaaaaac aggtttgcct aaattcccaa aagattcaat ataacttgca tcggatgtag
    26821 gtggattggg acctatctga cttccagcaa tcagttttgg atcaataata attccgttat
    26881 caatgtaatt taaaacagct tccatgcctc ttccagaatt acccgataaa gttgcccaga
    26941 aatttgtttt tggaatttgc actcccattg taaaattgga attaatgatt ggttgaacta
    27001 cactcgagcc accagttact gcgattacct taccaactaa cgcttgtatc acttgaccat
    27061 ttgattcatc aatagctcta ccaataacag gcatatatgg ctttatacct gcatcttttc
    27121 ctttattgat gatgatcgtt ctataaattg aattcagtct aatagaaaga acttctgcct
    27181 ttatataagg ataatcaact cttggactaa aaccaagttc ttttcttaaa agatcatttt
    27241 ctgcacgtaa ggcatttata tcttgaggta gtattttata ttcctcgatt aacttagcat
    27301 aagaatctct ttctttacga atcgattcgt aagattctaa tttattataa gcgcttttga
    27361 aaaaatttcc aaaggaatct aaagaagagg aaacagcatt gcttatcttt tgagtgctcg
    27421 caacaccttt tacaacaaaa ttaccattcc aaacaagaga ggatattgaa aacagaatac
    27481 aaaaaaggac agataagaat tctttaagct gactcagtct atcccataac atattgaatc
    27541 aatcttattt aactccgcgt ttaatgtatt tcaactcatc tagatatttt ccagtcccta
    27601 aaacaacaca tgtcaaagga ttctctgcac gaaacacggg aacacctgtt tctctagaaa
    27661 taaaatactc aagcccgcgt aagagacaac cgccacctgt taagataatt ccacgctcta
    27721 cgatatcggc tgataattca ggaggagttt tctctagaac tctttttact ccatctaata
    27781 tctggtcagt tggttctttt aatgcttttc taatttcatt actatctagc tctaaggttc
    27841 taggcaatcc ggagatagca tctctacctt ttacttccat agtatcagtt cgcttatcca
    27901 tataagcgtt tccgattgta agcttaatat cttccgctgt tctatcccca atcactagat
    27961 tgtattggtt acgtaaatgt tttatgatgg cttcatcaaa ctcgtcacca ccagttctaa
    28021 tagactcagc aatcaccata ccacccagag aaattacagc aatctcggta gttccaccac
    28081 ctatatctac aatcatattt cctgctggct catgaatagg gacatttgca ccgattgcag
    28141 cagctaaagc ttcttcaatt aagaaaatct ctctagcacc agcttgttca gccgactcac
    28201 gcacagcacg tctctctacc tcagtaattc cagaaggcac tccgatcacg attctaggtt
    28261 taacaaatgt ggtgcggttg tgcaccttag ctataaaata acgtatcatt ttctcgacag
    28321 tttcgaaatc ggcgatcacg ccatctttca ttggtcgaat tgctacaata tcacctggtg
    28381 ttctacctaa cattcgcttt gcttcctgcc ctacggctag aaccttacca gtcgatgctt
    28441 gtactgcaac aacactaggt tcagatagaa caatgccctg acctttgaca tgcactagtg
    28501 tatttgctgt acctaaatca atacccatgt catttgaaaa aagagcatat aaattatcaa
    28561 aaatcattcc ttactccaag tgtactctta aaataacaaa tctctaatat agggcttttt
    28621 gactacaaat tttaatatat agtcgaaaaa aggttaattt ttttgctttt ttacaaaagc
    28681 aactgcctca agctttaaat cactcccttt cggtgtttca actgaaatga tttttcgttc
    28741 aaaaaatgct gagttttttt ctaaatgctt tttgatatat ggattcatta aattttcgtt
    28801 ataaaaaata aatacatcct gtttttctat cgtcgcatag taaaaacttt catcaatttt
    28861 ataattatct tttagaactc ccagttcgac taaaagactc ttcgattttt tctcagtttg
    28921 agatttgttt aaacctgata actcccccgg aataaattcc cgaatattct tatttggaaa
    28981 cttttcttta agataaaaat aaggatcagg tatcgcttgc agataaacat tattatgcgc
    29041 ttcaactact ttctcaattt cagaaaaaaa tatatcaacc ttctctggca gtttataaat
    29101 aaagaaagaa ctaactatta tccatatgat tataaatacg ttatacccaa tggataaata
    29161 tacaaaataa cgactcaact taatttcgct aagtaaatag ctcagaatca aaacaaatgg
    29221 aactgtcata tgaaacacat accaaaattc acttgataaa aataaaaagg ttgtgatcgt
    29281 tagtaaccat attagagcaa aaaactcatt gtctgttttt atgagcttta taaaatattc
    29341 agctttatta gtatccttaa attttgtttt aataaaataa aacgcaaaca tgaaaacagt
    29401 aacaaaataa agactcaact ttatcaaagg gaatttataa acagaaaaaa taatttttac
    29461 tttatctatc caattaaact ttccaagtag ttcatttttt ctagataact gggctccaaa
    29521 ttgaataaca aacaaatcaa gatttggtat aatataaaaa agccaagcgg ttaatgggat
    29581 taaaccaccg atgcaataaa gaaaaattct cgatggtgta aaactgtttc tttgatacag
    29641 taaaaataaa attggcaaag agtgaacaat accaaaagga tgcgacaaaa aggataaaga
    29701 taaactaact gctgaaaaga ataaatcact tttagtagga tctaattcat tatgatctgt
    29761 ctgatttttt gatttgagta aaaaataaaa agcagataga gcaaaaaaag aacacaaact
    29821 ctccatacga gatgtatgag agaatttcaa aaatactaaa tctgtagcaa taaagaataa
    29881 aataaatcca attcttttta atgagaatct caaactctta gaaatctgat agactaaaaa
    29941 taatgagcca aggctaacga aagtgctaaa taaccttgca gttaataaat ttgctggaaa
    30001 gacttttaac aagagtgaca acaaaaccat gaaagttggg ggcatccaaa gtgtatgagt
    30061 atccatgcct ggaatcaacc catctaaaac ggatgttctc ataacaccat ttatccctaa
    30121 ctcaaaagca ggattgaaaa ataaaacttc atctggccag attggaggaa tatgagtatg
    30181 aaaaataaaa gagaaaaaga gaaaaagaaa aaaggaaaag taaataaagt gcttattaaa
    30241 cataatttaa aataagcact tgagggatat taaggtagag atgcgatagc ttcctcttca
    30301 gttgcataca catcaaataa atctaataat tcaacaacat caaaaacttt cttaacaggt
    30361 ggcgttatat tacaaagctt caactttcgc ccctgacggt ttaattctct taccatccca
    30421 acaaaaattc gaatcccaga agaggatata taagaaatcg atcctaaatg aatgataata
    30481 tcaccttctc ccgaaagaac atctttcgca agatgagatt ctacctcgtc agattgagta
    30541 atgtcaagtc tacctgcaag ttttaccaat acatgtttgc ctatattttg tgttttgata
    30601 tccaaaggat tctcctacga aaacaatttg aaaacaccta aatttaccta tattttcaca
    30661 attattctta gtcaaatagt gtttttttcg ctaacgtatt gtcaagatgt ttttttggct
    30721 agtggctcta agtaacagga ttcattacct tatttcaaga gttgctcaat agataccaaa
    30781 tcacctttcg gaaaacttcg aatctgacca aataaatttc gagttgatcg ggtattaaca
    30841 tgcccgagaa aaagtcctat ctctacctga gaccaaccat tttgtctgag atgaacagca
    30901 atacttctac gaatctttgg aacactaaca gaaagtccaa ttttattctc tattttttct
    30961 aaagcctttt gaatggttcg agtatgcagt ttaccggatc taccttgaaa aacaaaatca
    31021 tcagaagtct tttgatgaca aaagattcgg aaatcacaaa gtatagaaaa gggaatatct
    31081 aaagatctag ataaagattt ctccccacct agaattttta tctttgatct ttgaaagtca
    31141 atggcaccga cttttagatt aacaatgtct tttaaattca ttccaaatgt ataaagcaat
    31201 ttaaaccata atagatgagt ataatttgat tttaaaactt ttacaatttg aataatctgg
    31261 tctggagtaa aaaacgaatc atcttctact gtttcatcat atttcttact ttgtgtatct
    31321 gactttgcca tatttgtatc cctttatttt tatttatact aaagaatcct aatccaagta
    31381 taggactagt tggagtataa ttaattgttt ttctaaggtg atttgttttt ttcaaatatg
    31441 atttttattt atcaaagatt tttttctaaa aattacgcac ttcctaccaa ataagacata
    31501 attgtagagg ataatatata ttagccgcaa aataaagaat aaaacgcaaa cagatttcca
    31561 tttttatttt tctagcttga tcttcttata agatatttgt gactttttat ctttagaatc
    31621 ctttgaattg ttttcaaaat aaatttagat tctatagatg ttcgttctga aatcttaaaa
    31681 tagagagaaa ctctttaaaa ggtcctttct aatcagggtc tatttaaaag cgaaaggtat
    31741 cgggtgaagg aacaggctta tctttgtaaa aatctctttt ctcataaaag tttcttattt
    31801 caatctgtcc aggggctcta ggctttccaa tacaacaata agttgcgata gatccatacc
    31861 gatcaccaaa caacctagaa taaattccaa tatcacccat agcaatacca atgattgaat
    31921 tcttctctgc gttctctttt attccttcta agaaaatttt aagatcgata taacttttag
    31981 gcataacggc aaatttatag atacagcgct gagttctctt tttcgtaatc cagtttctaa
    32041 tttgtgaaga atctaagatt ccaaagaaat tatgatatga gtaaatcact gtaaaaaccg
    32101 aattggaaat attatcaaaa attgattcct cttgatccaa ttcaatatca atataattgc
    32161 tttcagaatt aaattcattt aatacttttt caatgtcttt gaattcaatt ttggtatttg
    32221 tattctcact cgaatctttt gggttgcggt aggtaaataa aattgattta tttaagcttt
    32281 ttaaaattgg acttaattct tcttttataa aagattgaga tagtaaatcc aatctaattt
    32341 ctaaaatatc tgcatcttga agggatggat tcttataatc cttcagttcc tccatcccca
    32401 atgttacaat tactttgtaa ttcatatacc attaactatg atattttttc ttatcttcga
    32461 tgattgttgc aacaacagat gggtcggcta aagttgaaat atcacccaaa ccttcaaatt
    32521 catttgctgc aatttttcgg agaattctac gcatgatctt tccggatctt gttttcggaa
    32581 gacttggcgc ccaatgaatt acatccggtc ttgcaatctt tccaatcatt ttttcaacga
    32641 cagcaattaa ttctttcttt aattcatcat ttgtggtaat tccattttta accgtaacat
    32701 aaacataaat accttgccct tttatgtcat gcggataacc tacgacagcc gcttctgcaa
    32761 cagccttatg agaaaccatc gcactttcaa tttcagcagt tccaattcta tgtcctgaaa
    32821 cgttaagcac atcatctact ctacctgtga tccaaaaata tccgtcttta tctcgtctag
    32881 ctccatctcc agtaaagtaa tatcctttga actgagagaa ataggtggat ttaaaacgtt
    32941 taggatctcc gtaaacacct ctcatcatag aaggccaagg agatgaaatg cataagtttc
    33001 cagtgatttc tcctttatct ttaatttctt ttccttcatt gtcgactaac actggcttga
    33061 ttccaaagaa tggcaatgtt gctgagccag gtttcgttgc gattgctcca gggagaggag
    33121 taatcatgat tgatcctgtc tctgtttgcc aatatgtgtc gactatcgga cattttcctt
    33181 ttccaatatt tttattatac cattcccatg cttctggatt gattggctca cctacagttc
    33241 caagaagacg aagggatttg agtgaatgtt ttacaatatg ggaagttcct tcttttgcca
    33301 atgcacgaat ggctgtgggc gccgtataaa aaacatttac tttgtattta tcaatgactt
    33361 cccaaaatct tcccggattt gggtaagtag gaactccttc aaacatgatc gatgtagccg
    33421 cgttagaaag aggtccatac aagatatagc tatgtccagt aatccaacct atgtcagcag
    33481 tgcaccaaaa agtatcttcc ggtttatggt caaaaacata atgaaacgtt agattcgcac
    33541 ctaacaaata accagctgtt gtgtgcataa ctccctttgg ctttcctgtt gatccagatg
    33601 tatagagaat aaaaagcgga tcttcggaat ttgctttata aggttcgcat tcagtagaag
    33661 cagtcttcat aagataatgc caccaatgat ctctaccttc ttgccagttc aaatctgctt
    33721 ccattccagt tcgttttaca acgatcacat ttttaatttt aacttctgac ttttgtaaag
    33781 caagatcaat gttctttttt atatcaattg cttttccacc tcgataacct ccgtctgagg
    33841 ttacaatgag ttttggttta cagtcttcaa ttcttccaat aagtgactca ggagaaaaac
    33901 caccaaaaac tacagaatga atggctccaa tccgagtgca agctagagcg gctatcgcta
    33961 attctggaat cataggaaga taaactaaaa cacgatctcc tttcttaacc ccttgtgatt
    34021 ttaatacatt tgcaaattta caaacttctc tatgaagttc ctgataggtg aatgtcttag
    34081 actcttcccc actatcgctt tcccagataa tcgcagcttt gttttttaga gatgaattta
    34141 aatggcgatc taggcaatta taagagacat tcaatttacc accttcaaac cattttactt
    34201 ttgcctctgc aaagttggct gttagtattt ttttaggtgg agtaaaccac gttagtcgct
    34261 ctgcttgttt tttccagaag gattgaggag atttaataga ttcattatat aacttttgat
    34321 actcttttaa attaatattt gctgatttaa caaaatcttt gcctggttgt aaaattctct
    34381 cttttgccat tgtctcacct ttaattatga aaaattattg ctctaaattc aaaaaaggtt
    34441 ttttatccta atctgtcaag actggttttc tgcaaaagta aatcataata ttttcatttc
    34501 atatttacaa agaattgata aggcattgac ccacaatgat gaatttttac atttctagga
    34561 aaaaagaaat tcacagaata agggcttcga ttttttatac aaccaatggt aattcttata
    34621 tgactcagaa gagtaaagaa acagaaaaac aaacaaatat acaacaacaa tcctttttat
    34681 catctattgg ttcgcttctt tttatcatca ttttagtttt tgggttcaaa tcctctattt
    34741 tagatgcaaa caatattcca tccggctcta tgataccaac tctcaagatt ggagattttc
    34801 tctttgtaaa taaaatgcgc tattcattca gaatgccttt tactgaaaag gagctttatc
    34861 gctttgatga tccaaagcgt ggagatattg taaccttcgc tcccccaaac agagacaaag
    34921 tgagagcatt gttaattgcg ctcaatcttt ctcctgatct ttccgtctac attccgccag
    34981 ctatcgaaga gaattatatc gaatccaatg gacctcagag attttttgga ttagcaaata
    35041 tgttttctaa acgttttgta aaaagagtca ttggtttacc gggtgataca attcgtctaa
    35101 cgcaaacaca agtaaagact acacgcggtg atgctgttag ttacacattt atcgaataca
    35161 aagaaaaagg ctctactgat tttaaaaatt acggtccaca aatacaacct tctgaaaaag
    35221 aactttctga cctagacaat gtaaatgcga tcggcaaatc actgtttact gaaaacaaaa
    35281 gagactttaa acactttaca atcgaaggtt cccacatggg gaattatact ttttatgaat
    35341 attgctctcg taacggtggt gtctgcacga ttccagatgg atattatatg gtaatgggtg
    35401 ataatagaga tgattctagc gactcacgtt tttggggact tgtaaaaaga gaagatattc
    35461 taggaaaagc tctcattatt tatttttcga ttaactggaa agattacacc tgtatgtata
    35521 aggaaggtgc tgggacagag tccgatcctt atcatgaaga aaaatatgaa aatgaagaat
    35581 tgtttaaaca ttgtcatgtt tccgaaattg atcgaacaat gatgaatgaa agtatctttg
    35641 gttggttaga tagaactctt cgttatagaa tctggcgtat ggatatccgc tggaaacgca
    35701 ttggtcgaat tttacagtga atctttggga gctatctagt ctcggaatcg aatttgcatt
    35761 tatatttgta ggctccgtct tactaggaaa ttatatagac gaaaagttta attttactcc
    35821 atggggcata atgttgtttg ccgctcttgg tttttccttt ggaatctatt atattgttta
    35881 cagagcaaat aaattagaat ctgacgataa aaaataacgt ttatgaatat tcaaattata
    35941 tcagccaact tagaggattt aaacgaagtt cttcctttat ttaatttata tagagatttc
    36001 tatgaaatga aaccagattt aaataaagca gaaagttttc ttcgagagag attacaaaac
    36061 aaggactcta aaatcttttt agcaaaaagt gaaaatattt ctgttggctt tactcaactc
    36121 tatccctctt tttcatcttt atcgatgaaa ccggtttgga ttttaaatga tctctatgtc
    36181 ctagagagtc acaggaaaca agggataggt aaaatgcttt tagatgcggc aaaacaattt
    36241 gcgaaagaaa caggtagcaa aggattaaca ctcacaactg cgattcaaaa tactacagct
    36301 caaagtttat atgaaaaata tggattcatg cgaaatgatc atttctatga atacttttta
    36361 ttttttaacg aatagcggct aattgagtgt atccgactct ataattccat cactcactcg
    36421 aattgttctt tgggctaatc gagcataacc ggcatcatga gtcaccatta aaattgtagt
    36481 tttatagtcc ttattcactt tctgaaacag actcaaaacc ttttctccat tgacggaatc
    36541 tagatttcca gttggctcat ctgcaaaaat atattcaggt ctcatgatga gagcacgtgc
    36601 aatcgataca cgttgcgact cacctcctga aatttgtccc gggagtttat gaatacaatg
    36661 tgacaaggaa aaatcttcta aaatatgttt tgcttcttcc attttctctt tgtgtaaatt
    36721 tgttttacga gccggcatca aaacgttatc taatacactg atctctggca atagataatg
    36781 aaattgaaat acgaacccca ttttgaaatt tctaaattgg tttaaatctt tttctttcat
    36841 ttcatgaatg ctttcacctg agaagagaac tttaccagaa gtgattttat caagtccact
    36901 gactgcatag agtaacgtcg atttaccgga accagaccga cccgtaacag atacaaattg
    36961 accttttgga atttcgaaat ttaaattttt taaaatttca ataggcggat caccaaacga
    37021 acgatttaaa tttatacatt gaattcctga aaacatcacg acccccgaat gatttcgata
    37081 ggcgaaatct tagccgctgc acgcgctgga atcatgctgg agataagaga agcaatgata
    37141 gtaagaaaca ttccttttag gtaaatatat atattgtaag ataccattaa gtgagtcgtt
    37201 cctgttgcta aattttttcc tttaaaaact tctatcgtgc tcacaaactt tgctaaaaag
    37261 tatccagcaa tcgatccggc aatagctccc gttaccccca gaatcaatcc ttgcactaaa
    37321 aataaaatta caatatcact ttcttcgtat ccaatcgatc ttaaaatcgc gatgtctctt
    37381 tttttattgt tcacaaccat atttaaaata ttataaattc caaaagagat gatgagtatg
    37441 attacaactg taatcgtatt tcgaatgata ttttgagtat taaaaacaga catgatactc
    37501 tcatttgcct gatcccaact ttctacttta tcttttgtaa actgagacca atcttctgac
    37561 atttcttttg cacgactaac gtctgttact cgcacaacaa tatcagaaat ttctccggga
    37621 gactctgtga ttctctgaag agttgtgatg gacgtgtaac aaatggaatc atccatcaac
    37681 ctatttcctg tttgaatgat tccaataatt tttagaggag taatttgctt tccgggaatt
    37741 ccaacttgaa taatatcatt tagccgcaca cccattttat tttttagatt tgctccaatg
    37801 atagcaaggg aatctccatt gtttaaactt tttaattttt caccttgcac gatgtattta
    37861 tctgtgtttg taatttttgt ctgttcttct gggttgatac caatgatttt agctggataa
    37921 atcaatcgtc cttgaataaa gctgacctgt ctgacaagct gagtagagaa ggcaacgaca
    37981 cctggttctt tgtttaattt atcataccac cattgaatat tactcaagcg actattgtct
    38041 gttttgcctg agggagcttt aatccaaaag attgtcttat ttggaaagaa tacatctttg
    38101 aaagtttctt ctgtaatggt ttcatctctt ggagaaattc ttatttgagc atcactgttt
    38161 acaagttgct caatgatgta ttcctgaaaa ccaaacatga tacttgtaaa tagaatataa
    38221 ccaatcgaac caattacgat cgcaagaaat gttaaaaaag tttgttgtgg tctagtaaat
    38281 aactgcttta gagcaagaaa gatcattatt ctctacctaa gaaaatttca tcatcagcaa
    38341 aaattccgcc ttctagaatt tccgctaact ctccattaac cgctccaatt tttactgata
    38401 cgacttccac ttttcccttt cttttaatcc tcacttgccc tctatgaatt gcatttgcag
    38461 gaatcagtaa aacattatct cttttcgcta cttcaattgc aacgtcagct gtcataccag
    38521 gaagaattcc ttctggtaga gaatctgtct ctaatctaac aataaattgc ccatcggaag
    38581 gatagacttt tttaacttta ccaatcagtt tagatccgcg caaattttca aaactgagtt
    38641 ctgctttttg atctttacgg attcgaagag cagaagattg atcaagagaa acttgcacga
    38701 aagttttgtt taaatccata acggttaaca ccgatatgcc gggcataatt gtttctcctt
    38761 tatccagata aattcgtgtt actgttccgg cgattggtga tgtaaaatca gatgactctg
    38821 ttgcaattag cttttgccct ttttctacaa gatcaccttc tgtaacgtaa agttttgtca
    38881 agccagatgt gatacccaat tttaaattgt aaatgtcatc ggattttaca gttcctaaag
    38941 cgtatatcgc ttctacaatc ggaccaactt gcggtttcgc tgtattttgc tttttaaata
    39001 aaaaaagata actaaaaatt gcgattatta taatagcaaa tccgattcct aaaaaaagtt
    39061 ttcgtttatc cattcgctcc caatgcctct ggtgatattt tattttcact cactagtgag
    39121 gcgacaaatt tacttttaat tttagaagaa caggaatgat ttatgtcata aagattttgt
    39181 ctaaaaagta taatttcctt tgaaaatcat ttttaaataa ttctaaattt ttaagcttta
    39241 catatcttat cgaatggata agattgttac gtgattttat cattcaaaga taaagaatct
    39301 gaaaagatat ggaactctga atttacaaag aaatttccca ttgaaattca aaagaaggca
    39361 aaaatgaaat tagaacttct aaatgccgca aaaaaattag aggagctaaa aataccacca
    39421 tctaaccgtt tacatatttt gcatggagat agaaaggggc aacagagtat ttcagtgaat
    39481 gaccagttta ggatttgctt tgtttgggaa aatggaaatg cttgtaatgt agaagtagtt
    39541 gattatcatt aggttattat tatgaaaaag aaaataaatg cagaattaaa tattcatcca
    39601 ggagaaattc taaagaaaga atttttagaa gagctaggaa tttcagctag aacgttatgc
    39661 ttagaaacgg gaattcctga atccaattta agtcaaattt tgaaaggcaa acgagatatt
    39721 actgctcata tttctctact cctaggaaaa tttttcggat taaacgatgt tttttttctt
    39781 acaatgcaga atcattatga tattacaaaa gaaaaaatga ttcatgagat aagactctct
    39841 aaaattcgtc cctatcataa attgaagaag tctgcttgat tttcttgaaa cgattggctt
    39901 acagaaaaag cagaaagttt aagttaaggt tttcttttgc tcttgacaat ctgaatttac
    39961 ttcagactat aacgggtcat gttctcaccc aagaaaagac tcttaaagat taaaaacgat
    40021 ggactccgat taacaccgat attctgcaaa gaaagaattg gaatgcttct tcgtcattgt
    40081 aaggaatgca tatatgcgtt ccctacttgt gcgattattt ttctcttctc catattaccg
    40141 atatccgcat tagatacaat cgatatcaac acatgtaatc cgaattgtgt aatcggaaag
    40201 aatttggaaa tcctagaaga caagacaaat acattgacga ttaacgatat tcgcgatccc
    40261 cgcagggaca aggcatgcct tgtccctgcg gatacgacaa cagaaccttg ttggaagaaa
    40321 agcgaatcag acaaaccaaa ttttggaata tctgcatctg cctattgggt gcgatttaaa
    40381 gttgcgaata atacggataa gccggtaaga tattttttgg aattgtatta tcctcaatta
    40441 gattacgccc atttttatta ctaccaaaat ggacatataa aagaaattgt tttaggtgat
    40501 agaatttcat tttacaaaag aactcttcct aatagaaatc ctttatttga aattaaaatg
    40561 aaagcccaaa aggaaggagt atactttatg aagtttactt ctcaaggcac agttacattt
    40621 ccactctctt tatgggagcc gaaagctttt tatgactatg accataatat gcaaatgtat
    40681 tatggattct attttgggat tctttttgta atggcaatgt ataatctttt tctttatttt
    40741 tctatccatg ataaagtata tttattctat gttttttact tggttggtat tagctctgtt
    40801 gcatcattac aggcaggttt tgcttatgaa tatttatttc ctgatagtcc aagttttatt
    40861 aatatatact tctttattag tattgcagag gctggttttg gtattatatt atttacgcga
    40921 agtttcttaa atacaaaaat tcagttacct aaagtcgatt ggattttaaa ggtttacttt
    40981 atccttttta tagttctttt cattggcggg gtgtttttac cttatcatag tgttattcct
    41041 ttctcggcat tgctaatgat cataactcca acgacttgtt ttatcattgc cttcatactc
    41101 ttacgaaaag gataccgccc agctcgtttg tattttttag cgtggtcttt gcaaatcata
    41161 tcaacaatta tgtttgcatt tagaggaata ggtcttttgc caacacactt tatcattgtg
    41221 catggctttg aagtaggttc agtaattgaa gtaatcctcc tatcacttgc actcgctgat
    41281 cgtatcaaca ttctcaaaaa agaaaaagag gaatcccaaa aagcgttaat agccaaacaa
    41341 gaagaagcaa ttgcaataca aaaagaatta atttcttcct acgcccgctt tgtccctgaa
    41401 caacttctaa catttctagg aaaagacatc atcacaaaag taggacttgg agattctgtg
    41461 caaaaagata tgacgatttt attttccgac atacgcgcgt ttacctctat ttcggaaagt
    41521 ttgagtccga gtgagaactt tggatttatc aattcttatt tggaaacaat gggaccgatc
    41581 attcgtaaac acaatggatt tatcgataaa tacattggag atgcgatcat ggctttgttt
    41641 ccacaaaagc cttctgatgc aatcgatgct tccattgaaa tgttagaaga attgcataaa
    41701 ctaaacgtta aacgaaaagc aaagggctat tctccgattt cgattggaat tggaattcat
    41761 acaggaagtc aaatgcttgg aatcattgga gaaaaagaga gaatggaagg gacagtgatt
    41821 tcagatgtag tcaataccgc ctctcgttta gaggggctaa caaaggcata ctccacagcc
    41881 cttcttgtaa gtgaagatgt tctagatgga gtagcggata aagacaaata cgaatttcgt
    41941 tttcttgata cagtgaaagt aaaaggcaaa aataaagccg ttcgtatatt tgaaattcta
    42001 aatggggtct ctcctcgaat ccgtgaattg aaacttcaaa ccaagccaga tttcgaagct
    42061 ggaattaatt tttattattc taaaaatttc aaagactcca tcaaaaagat gaaatcagtt
    42121 ttgaaaaaag actccaaaga taaagctgca gagctttaca ttgaaagatg taatttctat
    42181 tctaagaatg gagttgatcc taactgggat ggaattgaaa aattggattt taagtgaaac
    42241 tagtttcctt cgaaagattt tcttttaatc tctgaaaaat gattcgaaga tttgtttgct
    42301 cttctttcga tagattgtca ataaagtatt tatagatgca ttctttatat ttgacccaag
    42361 cttttttaac taagttactt cccttttctg taatttcagc atagtaggat cttccatcgt
    42421 cttggttttt atagcgggtt actagttttt ctttttctaa tgtgttcata atttttgaaa
    42481 ttccgctttt tgttaaaaga cattttttag acagttcccc aagagttctt ttcttttccc
    42541 gactttgctc taaaacaatc aatacatcat accaagcgag cggaattaaa ccagcaagcg
    42601 acatttcctt ttcgatttgg aacatcacat cagcatgaaa ggaaagaaaa cttctccatg
    42661 attcaacttc taaaattctt tgttcttttt tttgctcttt atccatagaa taattttaca
    42721 aaaagtagtt gacttgtcaa ctaatataat ttaaatagtt tttgatagtt gagtagtcaa
    42781 ctaattggag attaaaatga atgagatttt acaaaaactt tttacgactg ataatagctg
    42841 gattggttta atttgtcgac ttacaatagg gatcatgatt ttaccacatg gcgcacagaa
    42901 attactagga tggtttggtg gttatggctt tgatggaaca atgaattttt tcactgattc
    42961 tatgaaactt ccttatatca ttgcactgct tataatttta ggcgagttct ttggtggagt
    43021 agcattaatt ttaggagtag gaactcgttt ctttgcagct tgggttggaa tcatactttt
    43081 aagtgctatg tttattgttc acttagaaaa tggtttcttt atgaattggt ttggaaatca
    43141 aaaaggagaa ggtattgaat tttttattct tgtgctaggt ctgactatct ctcttttgat
    43201 tgaaggcggt ggaaaatatt cagtggattc tatattgttt aaatgataaa atgaaaattc
    43261 ttactctaat gaatcagtcc tcgattcatt agagtaagaa atcttctaaa aagaagattg
    43321 taaaagtctg attcaatcgg caaaatggaa ggtttcttgc ttagtataaa cttttacaat
    43381 tttaaatcta tatttctaaa ctttcttaaa atactctttt ttatttctaa taacgtccct
    43441 attcaaaaac gtgaaagtaa ctgattcgaa ttccgcgatc catcagtctt tcaggaataa
    43501 ctggaagttg accagaaaca gcaagatagc cacctttata aatttgataa ctcaatcccg
    43561 gatatacatt ccaaaatttt gtattcacat ctacaactgg gttgtaaggt tttcgatatt
    43621 ccgtttcagc gaataaccgt aatttatccg ttacaccata cgataatgca agacctgctt
    43681 gatagtatct tctgaattct tcttgcgagt tttctttaaa agctcgattg gtttccgatt
    43741 gaaattgtaa atccacgatt gcttcaaacg atcctatctt caaaccaaag ttaagatttg
    43801 gtcgaactaa atagagctca ggagcaacac catctcgggc atattgttta gattgtctat
    43861 caaaccacct aacaccacca ccccaaacaa aagcaccgaa ggagaattga tgttctctgg
    43921 caaattttaa tcccacattg agtctatcgt attgagttat cgtctttgat ccagatcttt
    43981 cccattggga ctttgtggtt cctactttga ttgaaaagta ttgtaagaat ttatactcgc
    44041 cttcaatgta tcctactttt tctctttgat aaatattgga gtcccttttt tctgatacct
    44101 gtccatcaat tctgactttt gtgtagactg cggctggttc cactcttaga ggaattgcaa
    44161 aagaactctg ttcctctgca gaaattcgaa tgatcccaaa taaaaatagt atgcttacta
    44221 gaattttatt tttcataatt agtatatcct tctaatacgc ttacatatct tttcgcgatc
    44281 aaactaaagc caccggtgtt cggatgaata cagtcccaca tatttcctgc cgctgctaca
    44341 ggaggtccac ctaacgtcct tctcaaatca atatatctca actgtggttc acgagttgtt
    44401 aaatctctca gcatattatt gtaatcagat aaaacatcga cttccaaagt attaatttct
    44461 gtatctgtaa aaccggctga gcgaagcgtt gctctacatc ctgtagatcc tggagcacta
    44521 acaacatctg gatttggata atcataccca tgtataatcc aaagcattgg cgctccccca
    44581 tatttttgaa ctttatatgc gttacccgat cgaattagat ttaaaagaag attttggact
    44641 gtattaaatc tagcctgttt ctcagccgca aatcttccaa catatgcact cggatttccc
    44701 tgaatatcat ttcctcccaa agataaaagc atgtatttta tatctggtcc tgcatcatct
    44761 attactttag tatagagtgc tgtattataa acagttgcca ttgtttgccc cgctagagta
    44821 gaacctacca ttttataatt gtagtttttt tctaactgca cacgtaacga ttcaattaaa
    44881 ggaactccaa gagcaaggtc agtccaacta tcacctagaa ttccatactt aactcctctc
    44941 tgtgtaggac atatcgcacc cacatagcaa agaaattgag atctaaattc cttgggcata
    45001 tcatgcttct ttgagtcaca atttgtaaga aaaataataa acaacaagat tgaaatgaat
    45061 ttcattcgat tcaccaatca ggagatgctg ttaaatgctt ttattacatc tgcaggagct
    45121 tgcactaact caactaaaac tccctcagag caaagaggaa attcttcatt tccttttgga
    45181 tgaataaaac aaacatcaaa accgcttgcc ccttttctaa ttccaccagg agtaaaacgg
    45241 acaccttgtt cgcctaacca cttaacgcat gcaggaagat catcaatcca aagtccaata
    45301 tgatttaatt tcggatcgtg aacttttggg cttttattag catcgatagg catcattaaa
    45361 tctacttcca ctgcaaagtg accagcaccg attctgtaga tgtcctcatc tacgttttct
    45421 ttttcacttt tataatttcc aattttggta agacccatga tgtctcccca aaatttactg
    45481 agtttgtctt tattctcatt tccaatagct acttgttgaa ttcctaaaac tttaaatggt
    45541 ctcattctaa ttctcctctt attaccatat atcaaaatta aaaattgata caataattga
    45601 aactctattt taacatgagt ttgatttaaa aatctcaccc agactgtttt tttagatagt
    45661 gtaaaatgaa tttgtaaatt tccttttgtt aaatcagaag ttaagttaat gaaaaaagtt
    45721 tccttctacg aaaacctttt taaataaatt ctaattcact ccatttcatt ggaggaaaat
    45781 tttaaatttt ttagtttatt tctttattcg ggcttgattc catactgttg cataacagag
    45841 agaatttcgt ctgaatcagt taatttatcc acattcattt caattacttc ggaagattta
    45901 attttatacc cactttctgt ctctgtcaga tttaaaattc ctatcttatc acctaacaca
    45961 tcaaaagtag agtaaacttt gactccattc acttctaaat atccagtttt attacttact
    46021 ggttttcgaa tggattcatc cccataaatt acaataaagt ttttatttaa ggaagttaag
    46081 cgaacagcct catctgtaga accatgtaga agcagaatgt ataagtccgc tttattttca
    46141 tcaatcgaga ttatattttg taaagtagat tctaatcctt tgataatgaa ttttcttttt
    46201 aactctcccg aaagtgtata ttgaatcgtt ggactcgtga gagaaatgat acctattctt
    46261 ttgtttcctt ttttaataat agagataggt tttccaactt tactcgatcc aaaaaatttt
    46321 tttataaata cattagctga atttaacgga taacgttctg aatctactat agaaactaaa
    46381 tcctcttgta aatcgttgat ccccacacct acagaaacat agcccatttt gaaaaaagaa
    46441 tgaaaaattg cttctgtctt attctttttg ctttttacat caagtgaatt tcctgtatct
    46501 actaaaatat ccttggaaga gtttattcct ttctgcttta aaaacgtatc tcgttttgct
    46561 agaccaacag atagtttcaa tccacagata caagaataca aattaccaga aagagaggaa
    46621 ctataaataa ttcgaaaatc ttctgcattt aaggaaatgg aaaaagttaa aatgaacaaa
    46681 aatagaattg tagggaattt gaaaaatttg tcatggagaa gctgttgaat ccatagcttc
    46741 tttggtttag atcgtccatc aacacgttca agagaacaat ttactagcaa gctgaatagt
    46801 tttgctttaa atttaattat atgcttatta tagaaaataa aattaatttt tcggatcatt
    46861 gtcttgtggc aaacctttgt tatcctctga attagagaag agtctatctt tgaaacgagc
    46921 atagacaaaa ctagcaatta caaataaacc accgatagaa atcagaacca aaactcttac
    46981 attttctttt aaattttgta aatcccagaa tgcaattttt aatacagcga gcccaataag
    47041 cccaatgcct gcataacgga tcagagtctt atttctatac actccatagc caataaatac
    47101 aattgcctct aaagtccaaa tcaaactcca gaatctaaca tcaacaacgg tcattgtaag
    47161 taagataaac tccaaaaata aagatagagt tacaatgttt tcaaagctat atttattcgg
    47221 aataaaatcc gtttgtttgg acttgtaagt ttctacattt tttactgaga ttagtaacaa
    47281 aagtagcgta aatgtgccta ttgtaaaatc tttcacattc ataccacccg atacatttga
    47341 attattaccc aaggtcatat atagaaaata aaccatagta agtagaagag gataataata
    47401 agaatgtaat acttccggga aatggtattt ctgtattata aatgcactga tgtaaacaaa
    47461 gatcgcaaaa aagaataaac tgaaatggat ttcagataca atatagatgg accaaaataa
    47521 aatagatata gttaagatat aataaataat tctatttcta gaaagagtat ccttagcaaa
    47581 aatgaaaaca gtttgcagaa tgcttaacac ggaatagcca ataattaagg ctactcgagt
    47641 cggatcctct tgaatcgatc cactcggaaa gtttgtatga tttagaaaat ataaaacact
    47701 gatcaaaagc gttaggtaag cataatcgta gtatttctgc atttgaaaac gaaacagaaa
    47761 aaagagatgt cccaaatgta aaatcgcaaa tgcaatcgat ctgtattcta ctggaaccac
    47821 aactaagata aaacaaaaac caattaatat actttgaata taagaaaaaa tacgagaaaa
    47881 taaatctaaa gtaaaaatat atataagtcc agcagtccca aaagatgtaa tcgtatatgt
    47941 tactaaattc atatattcat agagaccaac tgggcttttc tttgaaaaaa ccatatctgc
    48001 aattcccaga aacaaagtga agaaagttat taaaagtgag ttgtagataa aaattaaatt
    48061 ctcttctttc ttataagcca acactaacag caaactatta atagccgcat acgtaagtct
    48121 ataaggagga tcaatgaata tatctcctat cagcatagca gaaaatgtag tagcagaaat
    48181 tataattgga tgtaaaatct tttttatttc tagagtgatt ccaattttat ttgagttaaa
    48241 caaaacaaaa atccctgaaa tccaaaaagg aaatagattc gtaaaggcaa tcaatccttt
    48301 atcgcttaac gctagcttta catcttccct atataaacca tatcgataga ttcctaccaa
    48361 aaatataaac ccagataaaa gactaccttg ttttaatcct tgttggtatt tgtaatttac
    48421 taaaaaaaat agacaaacga tcccaattaa aacaatcgaa tgcaagtaag gtgaaaaatc
    48481 ttttaatact agaccaagta tagaaaaaag acttacccag agaaaagttt cccctttaaa
    48541 aaataagact tttaggaaag gaacttgtct ttgatcaata atatttacag ataaaatatg
    48601 agagtaacga cgtaataaaa cattttcttt cgcaagaaaa tattgaatcg agctcccaac
    48661 caaaacgaga ctaaaagaga ttgcttgatc tatttgattt atttctggac ttgatttaaa
    48721 aaataatgga atagaaagta aaaggctaat tatcattagc ccaaatgaag agtagtagaa
    48781 atttctattc ttagataaaa gacttatata aagtaataaa cctgaagctg gaatcaaaag
    48841 aaaagcttgg tatggctttt gaattaaatc aatagaaaat aaaacgagtg taaatgttgc
    48901 aaataaacca aaaccattat aggcaaattt atccaattta gaccaagctg agtagaattt
    48961 cttggaaaag agaatcattc cataaaaaga aagtatcatt aaaacatata acattcccct
    49021 attaaaatca aaatagaggg ttgggaaatt gacactttca aagatataca ctgctccata
    49081 tggaagagtg aaaaaacgaa ctaggtttaa aactaaaaca gtatatccaa gattctttaa
    49141 gtatgcttct ctatatacaa aacctaatcc aattagaacg atagactcta aaagccaacc
    49201 aaaaacatgt ccatactcac taaacttaga tggaatagcg agagtaatta acgttatccc
    49261 tagaaccaga cttgagttcc ataaaaaatc tcgtttgtat tttttgcctg cgaatgctcc
    49321 tattatataa atcgttccaa gactcatatt tacaataaaa ggtccccatt ctgaaggctc
    49381 aggcttcaca tatccaagca ttacaaaaaa agaaaatgca ttcacaatat taatggctga
    49441 gcaaactctc tcagtttttt tatccaccac tttcatggta aaagtggaaa taaaaaatat
    49501 tatccagaaa aagagtaagt ataaaatcga ttcaacataa taattgtgac cagtttcaga
    49561 gatctttatt aaatatccat tttctgttct tggaagtcca gaggcataga gccagtaatt
    49621 tgcatatgtc gcgatgattc caactccagt taaatacttc cacttcctga ttgtcataat
    49681 gacaattagg ctaacagcga gtaacccaac agtcgccatt gaaaaataat taaaatccga
    49741 attttcattt atggaaatag tcagaaagcc aagaaaataa gcgaaggaag tcagaatttc
    49801 ttcattgtat tttaaacttg ctcctattac aaaactagaa gttaggatta acaggataaa
    49861 gcctattagc ctctgatcag gagggaaaat acgtgtggct tcaatgttat aagaagcata
    49921 aactgtaaaa taaagtagag caaaagaaga agcgatcagt ccaagtccaa atgtagaata
    49981 ttttttagtt ctaaatattt tatccccacc aaaataaact ccaatcgata aaacgaacat
    50041 accaattact ttcgtaaatg gaccaatcca atcgtaagca agtcgtaaga aaaaactaaa
    50101 accaactaca agtaataaga gaccgatctt atttaaaaga ttaactccta aaaactcttc
    50161 taatggtata tttccaactt tagattgaaa ccagcttttt aaatcatcga taaagtcagg
    50221 actagactct tttctggaat aagtttcttt tacttgagac actggtctcg gtttataagt
    50281 gacttcttct ttttgcgtcg aattgttttg aagaaggctt ggtcgaataa tggttggctt
    50341 agtttctttt ggaataggtt gaggctctag tttttcttct tgaattgcct cataggattc
    50401 agtgattggt gacgttagtt ctggctctaa tgcggtatca ctggtttgtt cttcttttag
    50461 atttttttcg tatttctgaa ctgctaaatc aagctttgct cggagatgtt ttaaatctcc
    50521 ttgcaaagtt tccattttat tttcaatgga cgcaatctcg gaatagagtt tcctaagttc
    50581 gtccgattgc atttatcttc tgcgaccaaa gattcggagt aaataaagaa ataaatttat
    50641 aaagtctaga tacagtgtaa gagctcccat aattgcttct ttctggtctt cttctgttcc
    50701 ttcatttcca atgatattca tgttttttat tttctgagta tcataggccg ttagccccac
    50761 aaataaaata acaccaatat aagttgtaat ccagtaaatt gcctcactct tcataaataa
    50821 atttacaaca gaagcaagaa tcaatccaat taatgccatc ataaataaat taccaagctt
    50881 tgtaaggtcg gatttagtta catatccata aaaacttgta agtgcgaaag ttcctgcagt
    50941 aatataaaat gtagaactaa tagaagctga ggtataaact acaaaaatta tagaaaaggt
    51001 taaaccgtta agtgccgcat aaaaaagaaa aacgaaagtt gccacagttg cattcatctt
    51061 ctgcacaaga ccagataaag tcgcaactaa gatgaattct ccaattaata aaccgtagaa
    51121 tacgaaagag ttgccaacga ctattctaag tgctgtttcg ctagaggcaa ctaacataga
    51181 tacaatccca gttagtccta atgctactga catccagcta taaactttaa cgataaatct
    51241 ctgctgctcc gccataactt gagcgggact cattgaataa ctttgattca taaaaatatc
    51301 ctttataatt ttgttgcaaa tcgacgaggt ttgcctcttc tttctggatg tgttgttaag
    51361 aagtaaactc tcttttctaa ttcttctgtt aacatctttg cggctttctt ttctgcttct
    51421 tttggaaatt catccggtaa gattggaacc ccaatattgt atttgatgat agtatttcta
    51481 gtttgcccag agaggatggc ttgtggggtg gacatattcc atgcaccaga aattccactc
    51541 ggaataatag gaacgtttgc gcgtatcgca atttttgctg ccattgcttt gaatggtccc
    51601 atgactccac ttaaagttct agttccttcc gggaatatgg aaacaatttc acctgatttt
    51661 aaaatagata ccatgtaatt ttctaattct tcgtaataag caacagcgga ctttgcatct
    51721 tctccgtgat agttgcggat aatcggcatt cccccccatt tttgaatttg gtgagaatac
    51781 acatccccga tagtgtttaa atatttttct aatccctctt ttagaaaact gaaaggaggg
    51841 gatactaaag gagagttttt tgcattcatc attttaataa ttgcttcttg tggattgaag
    51901 acttcatttt taccaaggaa tacaactttt ctaggaatat aaagaccaat aacaggaata
    51961 tcaagtgagt ctgtatgatt gcataccaaa actgcaccac ccttgcttgg aacatgctcg
    52021 cttcccgtta cttccacttt ataaatcatt tccatgattc cttttataat cgttgctgga
    52081 acttctcggg gaataaaaaa aggttctaaa aattttgtaa tttcttccat ttataactca
    52141 ctctttaatc acagattaaa ttcaataggg gaaatgataa agtaattttt caagttccca
    52201 aaatggttta ttcgcttttc taatttgcat aaataccaaa tttacgttaa aaaaattgaa
    52261 tctagtaaaa atttctttta ttttaagaac tactttctta tctattaatt gagaggctac
    52321 aatgagcgaa attaaaacct ttggtgaatt aaaaaaaagc aattatatcg aaagatcaat
    52381 taaaatagaa atggcagaga atctttctgc aaaattagct cgaaaagaaa acatctttcc
    52441 aaaaattcat ggctatgaag atacagtgat cccacaactt gtaaatgcaa ttctatccgg
    52501 gcataatatt gtaatgcttg gagaaaaagg gcaagcaaag agtcgaatca tgagagcctt
    52561 aataaacctt ctcgatgaaa aaattccagt catcaaagga acagaaattc cagaatctcc
    52621 ctttcatcct atcactgcaa aaggaaaaaa gataatcaaa gaggtagggg ataacacaga
    52681 gttagtctgg ctcacaaggg aagaacgtta tggagaaaga ctcgcaccag gtgctcgaat
    52741 cggagatatc ataggagatc tagatccttc tcgtatcgta aatggcgaac ctctttcttc
    52801 tgaaggagca gttcattttg gtttacttcc cagaatgaat cgaggaattt ttgcagtgaa
    52861 tgaaatgcct gatcttgatt atcttgtgca agtttcgctt tttaatattt tagaagaggc
    52921 tgatattcaa attcgtggaa ttccaattcg atttccgtta gacgttatgg tttgcttcac
    52981 tgcaaatcca gatgattatt ctagaagtgg aaaaattata tcacagttaa aagatcgaat
    53041 tggttctgaa atcagaacac attatcccaa gacaagacaa atcggacttg agatcacgcg
    53101 tcaagaaatc aatcttcctc ttaaaacacc ggaagtgatt gttccaccat tcatcgaaga
    53161 aattattgaa gaaattacaa ttcaagctag aagctctcat ctagtaaacc aaaagtctgg
    53221 tgtgagtgct agactttcca ttgcaaacta tgaggtagcc gttagctctg caagaaaacg
    53281 tgctcttcat ttcgcagata ccactggata tgtgagactt actgatcttg gaaatatttt
    53341 cgcttcttgt tccggcaaaa tagaattaga cccgtataga gacgaagctg taagtgaata
    53401 ccaagttgta ttaaagctgt tagaacaagc aactagagtt gtatttgaag agtattttcc
    53461 tcctaaagca tattctcaaa attttaacga catagcaaag caaatttatg cgttaggcaa
    53521 attagagatt tcagatttaa tgcccgtaaa tagctacaga gcacttctga aaacagttcc
    53581 agctctctgg gatttactcc atgaaaaagg aatggatata gaagatcatt tatttgtcac
    53641 aggagtagaa tttattttag aagggcttgc ttccacacaa aagctttcaa gaagaagact
    53701 tggtgaaata tctaccttta aaacgataga cgcttattga tcacttcatt tttatttttg
    53761 ccaaacgaca atcattcaaa atatcatttt ttgtagattg tcatttggta tccccaaaaa
    53821 agaacgaaca catttcttag attatatagt ttcttaagat gcagaaaaag cttataaaaa
    53881 gtataaaatc tgcaaatatt taaggaaagg aaaaaggaga agatgctttg acttgttgcc
    53941 tgcatttcct tggttagaag aattttatat ctaaatccaa aaaaactttt tttaagagat
    54001 taacttttac tagcttttgt tatttttata agaataaatt tctttgattg tatcagataa
    54061 attcacaact gattccaatc aacacaacta caatctttac taaaataaga attcttttag
    54121 agccaattac tcttttaaat ccattaataa accacggatt ttttctaact gtttcaaaag
    54181 actaaatgca gaattttgtg ggcttagcat tgtagaggca agtatttgaa tattttcatc
    54241 aataattttt acagtcatta aaatcttttt atcatttcgt ccacgttgct ttgcttgcac
    54301 aagctgagta ttgtttttta aaatagaatt tgtgatatct ttcactaact ttttgtattc
    54361 ggttaaattt tcatggcttg gtaaattcaa aaagcgcttt tccatttcgg gaagtttctg
    54421 ccagagctca ttaatctctc tagtttcctc tttatcagaa ggaacaattg attctaatat
    54481 agataaaaaa gtattttctt tatctttgat ctttgaggaa ctatcagatt taattccttt
    54541 tgaattctta tcggctatcg ttttaggttt atctgtgtta atcgaaaatt gcattatggg
    54601 ttaaccgtgc atttgccctc gaagagaact ccttcttcga taaataattt tggagtaatg
    54661 atatctccat ataatctaca agtagataat agaaccactc tttcggaagc ataaatattt
    54721 ccatgaatct ctccacctgc cacaacaaca cgagctttta tatctgtatt cacaataccc
    54781 gtttttccaa taattacttt tccatctgtt tcaatgagtc ctctaaattt gccatcaata
    54841 cgaattaaac cagataattt aaactctcca taaaattctg atccttcccc gatgatacta
    54901 tttactgaaa actcttcttg ctcttcattc atataaaatt cctaattttg aacctgattt
    54961 aaaaaggagt aaggatttaa aggattagtc ccaacgtgga cttcataatg taagtggaaa
    55021 atagaactac cagatgtttt tccaacatat cctaatatat ctccctttga taccatttct
    55081 cctttcttaa cctgtaatct ctctaaattt gaatagattg tcttccatcc atatttatgt
    55141 ataatcttaa taaaataacc tgtagattct gtataacctg tttcataaat ttctcctggt
    55201 gcagttgcaa ctacttctgt ccctggaaaa gttcctatat caattccatt gtttattact
    55261 tcccgacctg taatagaaga aaaatatttt ccaaatggaa ataatatata acctcttgtc
    55321 ggccaaagag aaggtgtatt tttgataatg gattttcttt gtttaatcgt ctttataatt
    55381 tcttttgtaa gctctgcaga tttttttaaa taataaatat ctgattttaa agtgtatgtc
    55441 tctggtataa tatctgaatt ggaatcaaaa actccaagag gaagtttgga tttgtcagtc
    55501 attccaactt tatttggatc cccacccaat tttatataca atctagaaac tctttgatgg
    55561 tagtaggaac ttatatcatg taataaattc atttcagatt ttaattttag agattgcttt
    55621 tgaaaatctc cacttgatat tttcaattct tccatttcat ggacgcgtcc actgtgaccc
    55681 aaaacaataa ttgccgaggt cccaataagt cctccaatga tcagaaaaaa aattgtaata
    55741 gctttatatg taatactaat tttaaaactt ggtttctcgt tgtgtggtat aaccattaca
    55801 gtgagaaact cttttctttt ttttatgatc cgatctttag attgaatcag gaatagtttc
    55861 aagctattta gttcttcctg aatcttttta taaatagatt ggatatattc tttaatttta
    55921 aatttcacga taaaacctta atctaaaaga ccaaatgaaa gcttaattct aaaatgtaga
    55981 atccagaatt tttctgtatt ctacaagaat atctttactt tgacttttat ttcattacct
    56041 tcacatctct taatttaaga ttgtctctat ttaaaacacg aatcgatact tcattattat
    56101 cctgagatct tttagtgagg taaattttgt ttttcttaaa tgcaatttca caatcaggat
    56161 taattggatc gctacttctc atttcaaatg ttaagtcatc tttaaattga gctagatagt
    56221 atttaccatc cttaaattct acagcatata tcttgttatt ttgttctttt aaaaatgatc
    56281 tccagaaaat gacttcattt gtctgtttaa taagcttcaa actatctttg tctaaaagtg
    56341 ctaagtggtg agtatcagaa tttagaacgt catttgcctt atagctcata accacaactc
    56401 ctgttcccaa ttctttaaac tcttttccac aaactttttt aaaaggactc tgaaagagct
    56461 catcatcatt ttcagtatca actgcccaca tttcagaact ataatgacct tctgctaaaa
    56521 attccttaac cttcaaaaag acaatttttt ctcccactac gttttcactg ttttcgacct
    56581 tgtctttttc tttgattttt agctcagtta gttccttttg catttttttg atttcttcct
    56641 tcgttgtagg aagtttctca tcagccttct tagcaggtgg agctacgacg gttttaggag
    56701 cttctttctt tggctcttct actttcttag caggttcaac tggctttgac tcttttgcta
    56761 ccggtttctc ttcattagcc gctttttttg gttctgattt tgcttcgttg attgctactg
    56821 ttttagcgtc tttgctatcc tttgtagttt tagattcttc ttttgctgtt tctttcgcag
    56881 gctctttact ttcctttgca gcctctttcg ctggatttgc agtcttagaa tctttggtag
    56941 gctcttttac tgtctcaact agtttggcat ctttggttgt attcgaattt gatttttctt
    57001 ctttcggttt atcctcatta gccgctttct ttggttctga ttttgcttcg ttgattacta
    57061 ctgtttttga gtccttgcta tcctttgtag ttttaggttc ttcttttgct gtttccttcg
    57121 caggctcttt actttccttt gcagcctctt tcgttggatt tgcagtctta gaatctttgg
    57181 taggttcttt tactgtttca actagtttgg catctttggt cgtattcgaa tttgattttt
    57241 cttctttcgg tttatcctca ttagctgctt tctttggttc tgattttgct tcattgatta
    57301 ctactgtttt tgagtccttg ctatcctttg tagttttagg ctcttctttt gctgtttcct
    57361 tcgcaggttc cttactttct ttgacagcct ctttagcaga ctccttacta ctctttgagt
    57421 cttcttgcgg tttatttgaa ttttccgctt tcttcggttc tattgttttt ggatcttcat
    57481 cagttttagt tttcttagag tcagttgtat tctccttggc tttttcaacg agctccactt
    57541 ttttctcaat aattttcttc tcttctttta taacagttcc atcagtttta ttttttttat
    57601 cgtctggctt cttttttgtt tctgttatgt tttctttcgg aatatcagag ctagggattt
    57661 tttcttttat ctgtttgatt ttttcttttt ccactactgg attttttcct gttgtggtag
    57721 ttgtagtttc ttctttcttt tcctctttcg gcttattttc tgaaacctct ggaatcttat
    57781 taggatcttc ggaatatttg ataggaatta caatttgagt atttccaggc cactcactga
    57841 attttctact tattccaact ttatttttag tcaacgctgc aactaacgac tttgtataac
    57901 gttttttaat gcgatctaat ttatttctat tttgtgcgtt gtaataaaga acttccatga
    57961 ctaatacatc tgcattcttt tcagtgtatt cgaaggaact ttgaatgtag gcatttaata
    58021 ttcttttaat agaattgata tgcccataac ttgcattttt atttaatgca aggatatcac
    58081 caccaaactt acctttttca tcagagtaaa atcgctgtaa ggtgaaacct ttgtaattaa
    58141 ttggtttgtc tgtttgtata actagattgt gtaagccttt tccaatttct gcttctatcg
    58201 cttttaattc cggggttgca aatttgtcag aacgattttc aaattttgca tagggagatt
    58261 tacggatttc ctcttctcct aaaacaactt tcgactgagc atatagggtg gcttgtaaaa
    58321 gaaagactat cgtgattaga taaatatttt tcataaaaaa attcttttcc taatttaata
    58381 ttcataaatt gcaagtaaag tgcaatcctt ttacatttca gattgacaga tttttttgaa
    58441 aacacgatat ccttcccatg agtgatattt taaacaaagt aaaaaaagca atagctatag
    58501 aagtaaatgc gatcaaacac tttcaagaca cgctagatcc caattttgaa tctgctgttc
    58561 gactcattct agactcaaaa ggaaaagtaa ttgtcacagg tgttggtaaa tcaggtgaca
    58621 tagctaagaa aatatcctca acattgagct ctacaggcac accggcgatc tttttacatc
    58681 caacagactc tgcacatgga gatgctggtc tcatagcaga aggagatatt gtgatcgcaa
    58741 tcgggaaaag tggagaaagc gatgagttaa tagcacttat ccccaccata aaaaaaattg
    58801 gagcaaaatt aattggaatt accgctaatc caaaatcgaa attggcagct gcttgtgaca
    58861 taactttaat cactcctatt ttacaagaag cttgtccttt agcactcgct cccacatcaa
    58921 gcactactat tgccttaatt gcaggggatg caatcgcaat ggcacttatg gaattaaaaa
    58981 attttcaagc agacgatttc gctttgtatc atcctgctgg cagactcgga aaaagactaa
    59041 gtcttgctat cgaggacgtt atgcgatctg atgatcaaaa tgcatttgta acaccagatg
    59101 cagatctatc aatggttttg aatgaaataa caacaaagcg tttaggagct gttgcagtta
    59161 ttgactcaga acaaaaactc attggaatta taacagattt cgatataaga aagtctctca
    59221 cagatggaaa tttgacaaaa gaaaaaaaag caaaagaaat tatgaatcca aatccaagtt
    59281 catttactct tggtatgaag gcatacgatg ttttagtcgc aatggaagat agaaagcatc
    59341 caatatcagt agcgcccata accgataaac aaaataaact ggttggtatg gtcctaatac
    59401 atgatttact ccaaaaagga ttatgatcta gtttctttca tacactcaga ttgtttaatt
    59461 ttttattttc tctaatcggt tattcgtaga tacccaaaaa ggtttatttg gtttattttg
    59521 aaaagagtct acgtaaatcc tctaaatgga ttggtttagt taggtattca tcaaaaccaa
    59581 cttttaacag tctttctttt tcgcctgtta atgcatgtgc tgtcaacgct actatataaa
    59641 catctttata attttgatca gacttaattt tacttgttgc ctcaatacca tccatctcag
    59701 gcatttcaat atccattaaa atgacatcat agtttttttc ttttaattta tttaatactt
    59761 caattccagt tttagcaata tcagctttta acgatagtcg ctttagtaat gcagaaacaa
    59821 ttttttgatt tagatcattg tcttccgcga tcaatacatt aatatctttc cggatttgat
    59881 ctgattttat actttttact tcctcagcta cttcattctt cttgcttgca tctaatttta
    59941 aagtaaaaat aaattttgca cctttgccaa gttcgctgtc tactttgatt tcaccactca
    60001 tcatttcaac taaccttttt gtgatagcaa gccctagacc agtccctcca aattttcgag
    60061 ttgtgcttgc atcgacttgt gaaaaagaat taaacaaaat acttttctta ttatcatcaa
    60121 ttcccactcc agtatcttct acaataaact gaatgagata agaatcatta tttttctcta
    60181 atatcttgga ttttattttt attccacctt tgaaagtaaa ttttatagaa ttactaagca
    60241 aatttaaaaa tatctgtctt attcttactg gatctccttc tagttcaact ggtaagtcaa
    60301 tgtcttgttc tacgtttata aatagacctt ttttatctgc atcaaacttg actatagaaa
    60361 taacgtcctt aatgatttgg ttaatattta cagatatttt ttctagttca attttattcg
    60421 cttcaatctt cgaaaaatct aaaatatcat ttattataac aagtaaattc tgtgcactaa
    60481 ttcgtattga ttcaaaatat tctaaattct cctttgagaa attttctgtt gatatcaact
    60541 gtaaactacc aataatccca ttcataggag ttctaatttc atggctcata tttgccaaaa
    60601 ataaactttt agctctattt gctgattctg ataattttct agattcttcc aattcttttg
    60661 ttctctctat tacttttgtt tctaaatttt caaacagtat gcgtaaatct ttcgccattt
    60721 tataaaaaga tctagataac tcacccacct catcttctct ttttaagctt gatagacttt
    60781 cgatatgact ttcattgaat tgatttaatg ataaattctt tgcaacaata ttaagttctt
    60841 gaattggagc tagaattaat ttagaagagt aaataccgat agtcaccata agcattatag
    60901 caatcataac gatcactgta ataatacgat tgttcaattt aatctttcct aaaacatcag
    60961 aatcaggaaa gatagtaatc atataccact gaattccgct ttcagaaata tcatgatact
    61021 ggacccaata ctctattcca tgataatcaa gggtaaagtt tctattactt tttaatttct
    61081 ggatttgagc tgctacttct acaagaagtc catcattcaa ttcattcaat ttataaagaa
    61141 taaaattgtt atcatcaata atctttaagg tttgcatttg aactgatgaa gccaaaatct
    61201 ccccttttga atttacaata aaaacttttg aatttggggt agctaaattc ttttttaaga
    61261 aattagaaat atttgttagt aatatattaa caccgctaac tcctaccaat attccttttt
    61321 catctagaat ggatttaaaa atagtaattc ctaacaagcc agtattcgct tgcacaaata
    61381 cgttatttgt tagaatcata tttggattct ttactgcttc tctaaaccaa ggtctattta
    61441 ctgtatcata actttgtgtt ttaattaatt cttgcttctc tcctttttca tttaaaagat
    61501 aaaaaaatct aattttttgc tcgtttgttt cacggaagat atatttatct ttctgccttt
    61561 caattgcatg cagtcgtccg tttttttctg caaagaaaat atcattgata tcaggataca
    61621 atttaatttg ttccataaaa tataggggat aattaaaatt aaccccaggt ctcattaagc
    61681 caaatttgat agcttgtgaa ttttgttgga tcaggtttct ggaattaaaa aagtaagttc
    61741 taatatattc tttggttctg gtttgaatct gttcttggat tttttctgtt agatcatatg
    61801 ctgttttttc agaattccaa tgaaggaagc cccaaacaat cccaagcgta aataaaatct
    61861 ccaaaataaa tggaataata atttgttctc ttagaacaaa tcttccatga aagctagaaa
    61921 ttaatttacg tataaatctc aaggtaatta tcctttagca acaactctct aattagttgg
    61981 tttattccta aaagattatt atttcactct ttttttaatt tctttataaa atttaaaagt
    62041 ttttctcgaa tgaattcatc atcctctttt tgaatcgtct ccactaactc tgatttacct
    62101 tctaaagtct caatttgttt tgttgccttc tgggggggcg tatattgcat gtttcctgta
    62161 gtaatagtca attttgaaag gagtggagag gcaaatagtt tgttagctgc ttttaatatg
    62221 gagtctttca aaaagccaat ttccatttta tatgcagagt gagcgaccct taaaaataat
    62281 acatcccctt ccactttata cggttgcgcc tgattcacaa atacaggacc aatgatatct
    62341 ccccaatttt ctttgatttg atttaatata actgtgttta taagtatttc tttattatag
    62401 ccaatttttt gaatgactgt ttctaaatca gttaattgaa ttttgtgaat tggatccatt
    62461 tactctacct gggaaatatt tccatcttgg attaagaaaa cttctttaga ctcttgtaaa
    62521 tttccaatat aatcttgaat tccttccaaa tccgttgtag tgaaaaaagc ttgtccgcag
    62581 tccattaaaa gacttacaaa atactctctt cttttgacat ctagttcgcg aataacgtca
    62641 tctatcaaaa gtataggagc ttccccaatt tctttttgta taaactgaaa ctgagaagtc
    62701 tttaaagcaa tcactgtgct tctcttttgt ccttgagatc caaactctat aatgtcttta
    62761 ttatctcttc ctacaaaaat atcatctcta tgaattccaa cagatgtata acccaaatga
    62821 gtatctctag aaattctttc agataacttt tttgcataat cttcttccaa agatacgtta
    62881 ggcttgtagg ttatatgaaa gtcatcctta ttgccactga gcttggataa attttccctg
    62941 taaataatat ctaagcctgc aatcacgcga gctctctctt tcgaaatatc tgtccctttt
    63001 ttaacaagaa gttggtccca gggaagtaat tctgattgag aaaaatcttt tttcttaagg
    63061 agagtatttc tgtgctttaa gattttatta taatcaagta tatttgcaaa ataattgcgg
    63121 ttaacggtag aaataaatcc gtctataaat cttcttcttt cggaaggacc gccatcaatg
    63181 atttttaaat cgacaggaga gaaaacaaca gctctcattt ctccgatgat atcttgtttc
    63241 ttttgaatga catcaccgtt taactttact tttcttttga gagcttctcc cttgctatat
    63301 ccaatttcaa tcaccttatt ggtttcctcg gaagaaaact cacctttcac aaaataatgg
    63361 ctttgtttcc agttgattaa ctcttcatcg gaattatctc gaaaactttt taatgttgca
    63421 aatagattaa tagcttcaat gatatttgtt ttaccagctc cattttctcc aataaagaaa
    63481 ataagtttag atttgaattg tagctgaatg tcttcatggc ttctaaaatt aagcaatttt
    63541 agttttgata aaaacatttt tcactcagtt aagaattttt agccaacgat gaacacggat
    63601 aaaaactgat gggtttaatt tttattagcc ttttatctgt gttcattttt ttacaggaga
    63661 cgggtcattt aaacttatat cttcattggc attatgactg atgtatattg aggatccgaa
    63721 ggatctttaa agacagcagg taaattggat gaagtgaatt ccataatgat ttctgcatca
    63781 tcaattgatt taattacatc tgctaaataa tcacctttga atgcaataga catctcctct
    63841 cctttatagt caacaggcaa actaacattt acctctgttg atccaggagt tgaggaattt
    63901 atatgtaaga ggttttcaga gaaagtcatt cgaatttgtc tagaaggttc ttctgctgca
    63961 atcaatgctt gtcttaatgc tatttgcaat tcatctttat tgattcgaac agagctcttt
    64021 gacttgcttg gaataactgc ttcataatca ggataatttc catctattaa tttacaaagt
    64081 aattccacat ttcctatttt tagatagatc tgattgtcta cgattcctat ttttccagaa
    64141 tcatttgaat caatcatctt aactatttct ttgatcgctt tgtgaggaat aataacgcct
    64201 ttactaaaag gaagtgcgtt aggtatcact ctatctatct tcgcaagtct tctaccatct
    64261 gttccaacaa aggaaatttt accttcgttg gaaataacat ataaaccatt aaaaacaaat
    64321 cttgtgtctt ctaatgcaac cgagtaggat gtctttttaa tcatttcaga aatagtgaaa
    64381 caaggaaagt caacaacaag agaatcatca accttagaga tagtttttat ttcttctcct
    64441 tcaactccat tgatattcat tttaaaatct ttcttttttt ctgagtcagt tatagtggtg
    64501 ctaacgtttc cttctgcatt atcatttgta agtaaggctt ccggaaaatt aattgtttta
    64561 aaggcattgc ttaattgttt tgctgggata gatgttgttc ctggttcgat agtatctgca
    64621 tttacagatg ttttaattga aatttctaaa tctgttgctg ataaaaaaac ttttccgtct
    64681 tctgcttcca cttttatgtt ggaaagaata gactttattt ctctgacagt aataactcct
    64741 tccacagagc tgatagcttt ttgaaaatca tttgtcttta ctttgaattt catttcttac
    64801 atcctatcct atatttatat tttatattct tttgttgttt tagttgtaca cgtaaataag
    64861 tagataactt aaaatcagta tgtttttcta aggaatatgt cacattgatt tttgaaaata
    64921 actttttttc ttttctgact taattgcatt ttaagaggtt ttcgatttga ttctgtaagt
    64981 ttattatgtc ttatttacag attaagaaac ttaattatgt cttattaaca cttttgtgac
    65041 ataattccta catacttctt cttttctttt accaaggaag gttcttaagt gaaaggattg
    65101 agtgtcagaa aaatgaagtc aggaaactca atgcttgaaa accattgcag ttgagaaaga
    65161 aacctaactc ctttttcaat ttcccaaccc tctttctttt acctagtaac agaaaaatta
    65221 aattagaatc tattctcttt taaatgtttt tttatctctg cttgtgatta ggtaaacttt
    65281 ttgcatatgt atcctctgca attctgttta ttctaaaagt ttcgatgatt ttgtcttaga
    65341 atagaacaag attgtaataa cttttagcag atttattagt ttttatccaa gcaggtaaac
    65401 caatgactta catttctatt agttcccaat tgattgatgc cacaacaaaa atagtgaata
    65461 tactcagaca gattaaagac attaatagca ctaccaaatt aattgctatc aatgctgcga
    65521 ttgaatcttc tcgatctgct caattagctg ataatttctc ttccttgtct gaacaagtaa
    65581 gaactctctc aactcgctcc gaagaatctt ttagtgaaat ccaaatctta atagaaaatc
    65641 ttagagtttg ttgcgcaaaa gccattgcgg ttcgattagc agatttatcg atagacatca
    65701 ttgataaaat agatcgaaat ctttttgaaa gaaattgtga ctgtcaagct tgggcaacct
    65761 tcgaagacaa tgtgaatgct tgtctcactg ggaatgggca agatgcaagt agcttattaa
    65821 ataatctagt gaatgtgtat gaagtgtatc atgatatatt tcttttaaac aaagatggct
    65881 atgttatcgc aacaggaaag aatccgcagc ttttaggaca aaatcaatct gaccgagttt
    65941 ggtttaaaga agtgattcga aacaattctg ttcatgttac tgatatgtat ttctctgatt
    66001 ctgttggaaa ccataccgtt agttactctg caccagtcag agatcaaaat aaaaatatca
    66061 ttggagtttt atcgactcgt tttaattggg attatgttta tgacatcatt gaaaaagtca
    66121 aattagagag tcattgtaaa atctttttaa tctcatctgt aggaacaata atctcttcaa
    66181 aacaaaaata tgatatattg cgagataata tgctttggct cggtggtgga gaaagtgcaa
    66241 ttcaagggaa acgtggatac agtatagaga ctgcgcgcaa tggacaatgg aaggcttatg
    66301 gtttttccag aactaaaggg tataacgcat atgcaggtaa aggttggtct gttatagtgg
    66361 atgagccaat tcatctagaa aatcctagag tgatcataga atcgttttca gaaataagcg
    66421 agaataaaat cggaactaga tctagagcac actcagaaga tgtcaacaat caacttttgc
    66481 aaataacgca ccaactaggt gaatcaattg aatcaatcaa taaaatcaat aatgtaacga
    66541 caactcttgc gctcaacgct gccattcgtg cagatagagc aggtgatcaa ggtagagcat
    66601 tttctgttct ggcggaagaa gtgagagctt tttctcaaaa atcagatcaa ctgactgagg
    66661 aaattaatac aacacttgat tctttaaatc aagttgttcg agaaacagtt tctacacgtc
    66721 tagctgatgc tgcttttgat acgattgata aggtggatcg aaatcttttt gaaagaaact
    66781 gcgatgtgca agcttggaca acctttgaag aagtgattaa ctgtgcggaa actggaatca
    66841 acagagaaga aacttctgct ttattaaaag atctacatac aatttatgaa gtttattatg
    66901 atatctattt attaaataca aaaggtgtga tttgtgctgc tgcaattaga caagatctaa
    66961 ttgggcaaga tcaatcagat agagattggt ttcaaaaagc aattcaaggc tatgtaacag
    67021 tgacagatat gtataaatcg gatacgatag gcacatatac tgtttcttac tgtgcgccgg
    67081 taaaaaataa aaacggtcaa attgtaggag ttctcacaac tagattcaat tggaacttca
    67141 tagtagacat tatcaatgca acgcaagttt acagtgattg caaagtctat cttctaaact
    67201 caatgggaca agtgatcgca tcccaagata atagagaaat tttaaaaaga gattttagta
    67261 aatatgaagc ttataaatta gcaatggaag gaggaaatgg atttattgta gaaagagatg
    67321 ctgataccaa tcaaatttat ctagtcggtt atgcaaaaac agagggatac aataaatacc
    67381 aaggaaaagg ctggtgcgtt ttaattttac agcagaaaag atttgagtag gagttgccat
    67441 gtctactgaa ctaacgacca caaatttaga actagaatct ctttctgaaa ataaaaattt
    67501 tatattttta tttggagctg cttacttttc cattcagttg acaagtgtag tagaagttta
    67561 cgattcaccg gaaataaaaa atctcccttg gcaagagcta ggattaaaag gaatgattga
    67621 gtatagagga ataccggtcc cagtattaga tccattagca attgcgaata taacatctga
    67681 tcgaggaaat aaaccaatta aaacggtagt aatttttgaa aaagaaaatt gtaaaattgc
    67741 tttggctgtt gataaatttc acaatataat tccagaacca gagaacgagt cagaagattt
    67801 tcaagaagat tcctttgaat cttttattat tggtttctcc atgttatctg aaaaaccgtt
    67861 agtctactta aatgaagaga aaattgtaaa acattacaag aaaatattca caagacaagt
    67921 ttcactcaaa tcgaatcaaa aacaggaaat cttttcaaca gagaaaaaac aaattttaga
    67981 aaagtttatc tttttcacaa ttggagaagt aaactttgga gttccaatca gagaagtatt
    68041 agaagtttta gaaaacttag acgtaactcc actctttaaa gtagttccta cacttcgagg
    68101 attgatcaat ctaagaggaa aggttgttcc ttgtatggat atttccggtt acttgggatt
    68161 acccgctcgt tctttaaatg agaatacaaa attcgtctta ttgcaaagtg gtggatccga
    68221 gatagcactt tgtgtagatt cggtttctaa aatgaaagaa atcgataatg ctctcattca
    68281 aaacaatgaa ggtattttaa acggaccgat cagtgagtat gcgacaggcg ttttacaatt
    68341 acaaaagcaa actctactta tgatttctgc aaagaattta atagagtcca atgatttaaa
    68401 agaatatttt ttaccggagg cagaatagat gtattccgaa gaaattctat ccctctggaa
    68461 agaagacaag ccagcagaaa atccattaat agaagcacaa agcagaaagc tttttaaaca
    68521 atttattgga aaatctctca atgatgtgca aactatctta aacaaagtag ctggtttgga
    68581 aattagtaaa aataaacaaa gtgatttaga ttctgtttat aggatcaatc atacaatgct
    68641 tggattttcc aagatactta gaattcaaaa actaacgcat cttacttcga ttctagattt
    68701 tatttttgat tttgcaagaa aggagaattc agtttccaag tattcgattg attatttaat
    68761 aaaattaatt ttgaatgtag agattaaaat tttaaatcaa ttgtctgatt ctggatttat
    68821 taaagaagat atttccttgc ttgtagaaga agcaaaagtg tatttatcca aaccattaga
    68881 agtatggaat gaaaaacaaa aacaatccca gatcgttttt gctaaaaaat cttatacaat
    68941 tccagctccc tattcttcag aagtaaaaac aagtgaagag gataatcaaa ataagccacc
    69001 agctgaatcg attttagaaa gtaaacagaa tttagaagtt agtattggaa aagaatcgca
    69061 agaagagcct attaatgatg agccagaaga tttaaatatt ccgatcgaga aagttagttt
    69121 aatcagcgat ttttatgagg agtgttacga aaatttatcc aaaatcgcaa atcgcttagt
    69181 cgatttagag tctactcctg attccattga aattataaat gaactcttcc gttatgtgca
    69241 taccgtcaaa ggaggagcta ggcttttaaa aattgtaaag attgaaaaat taggtcattc
    69301 cgtcgaaaac cttttagatt tactcagaaa tcaaaagatg gaagtaactc ctgtttttat
    69361 tgattactta ttggaagcta gatctgccat gagtgagatg attgaagaag ttgcctccaa
    69421 aggaccgatt cgaacccgaa ttgcaccact cgttcgaaag ctaaatgacg cttgtggaat
    69481 ttcttcttct aaaccaaaag cagcagaagc aaaggcaaat caagatttac ctccaaaacc
    69541 ccaagtatca ttagataaag gaacgaaaga ggaatccgta agtgttaaac aggaaaaaac
    69601 gatagtagca caatctgaga aaattcctag tccagtggtc agtaaggaaa aagagcctgt
    69661 tgttcgaata aaagaaaggc aagtggaatc gattcgagtg agcattgata agctagatga
    69721 cgtaattaat acagcatcag aattatcgat ctcaagaatt caattccaag aacaaattgc
    69781 aaatatcaat cgaatgacaa gagatataaa aagaagtctt gcaaatgcag aagcctttga
    69841 ttctaaaaga ttactcgata gaatttccaa atcgaatcaa tgtcttattg cagatattaa
    69901 ggcagagatc ctgaaaggcg atggggatat aaatgaaagc atccttactg atttagttta
    69961 tcgatttcac aatgaaatga aatctgagat cgctcgaaat gaattaagtc aacaagaaga
    70021 actaacgtta atactaattt cttttcaaga attaaagaat ggaatgctaa agaatgttga
    70081 gaatttagaa aatcttactt ctaaattaca aaatgaagta atgaatttta gaatggttcc
    70141 tatttcaacg ttatttgaaa gatttccttc tcttattcgg gatatggcaa ggcaagccgg
    70201 aaagaaagtg aaaatggctc tttatggtca agaaacagag ctagatagag ttatgataaa
    70261 tactcttcta gatcctcttt tacatatcct tcgaaactca atcgatcatg gaattgaaag
    70321 cacggaagat agaatcaaag taggaaagaa tgaaacgggt agtattaatc taagtgctta
    70381 ttaccaaggc tcatatgctg tgattgaaat caaagatgat ggaaaaggaa ttgatactga
    70441 tgctgtttta aaaagagcaa tcgaaagagg gcttgcgaca gaagatcgga taacaacact
    70501 tagccagaaa gaaattttag aatttatttt tgctcccggg ttctctactg ccgagaaagt
    70561 gacagagctt agtggaagag gcgttgggat ggatgtagtc atgtctacta taaaaggtct
    70621 acaaggtagt attgacattc aaaccaagaa aggacaaggc acaactctac tattaaaaat
    70681 tccactgaca cttgcggttg taagagtttt actttttgaa acaggctcac agcttttagc
    70741 atttccaatg accaatgtag atgagatatt aactatctct agagatgaaa tcgaaacggt
    70801 aggcgctaag atgctttacc atctgagatc cgaagtgatt tcacttgttc ctctttctga
    70861 tattttaaat acacaacagc cagtcttttt caaagaagag attcctgtga ttatattatc
    70921 tgatggagtt aaaaaagtag gtctcgttgt agatgcactc ttaggaagac aggaaattgt
    70981 gataaagaat ttaggtaatc ttctaaaaaa ggttcctttt attatgggtt gcacaatact
    71041 aagcgataga cggcttgtta taattttaaa tccacgcgag ataattgata ttgcatcgga
    71101 tgaaaagaat gatttagata aaaagactcc ttctcttaaa attcttaaaa aagaagagcc
    71161 tcaatccatt ctagttgtag atgattctcc aatacaaaga aaaacaatta aagcaatctt
    71221 aactagagct ggatatgcag tggatgaagc agaaaatgga tttgatgcaa tgaaaatggt
    71281 tcgggtaaag cgttatgctc tattttgtgt agatttagtc atgcctttga tggatggatt
    71341 tgatttagtg agtcgattga aaaatctacc actttataaa agcattcctg taatcgtgat
    71401 tacatctaaa aactcagtag aagacaagga acgtggaatg aaaatcgggg cgagtgaatt
    71461 tttagagaaa ccagttgatc cggatattct aacagactta gtaaaaagat atataggttc
    71521 agaataatgg aagagattaa ttataaaaat acaaactatc caatttatct ttgtgtggat
    71581 aatatactat taaccgagta tttagaaaat gagttatctt ataataaatt taaatttgaa
    71641 aaaataaatt ttcctgatct ttcattgaaa atttctgaat tgagaaaatc actcattatc
    71701 ttacagacgg atgaagatga ataccactta attgagcttg ctcgtagaat taaaatgttt
    71761 tatgggtatg aaactaggat catttttcta tctgcagatt ataagacaga agaagatgcg
    71821 agtaatgtta cagataaatt ttttcaaatt cctgtaatgt ttaaagaaat cgaaaatgcg
    71881 attcaaaagt ttttaacaga tacacataag attttagtaa tagatgactc taagttagtt
    71941 cataaccact tagttccacc ccttgtgaat gaaggctata aagtctatga agcgtttaac
    72001 ggtagagaag gactagatct tacaatcgaa tacaaacctg atttgattat ttgtgatatt
    72061 gaaatgccaa acatgaatgg atttgaggta tgcacagaaa ttagaaagca tcctgatgtg
    72121 agagatactt atataatcat gtcaagcaca cttggttctg cttctgatat ccaaaaaggg
    72181 ttcaatgcag gtgtagatga atatatcaca aagccagttg tgattggaga attgcttgaa
    72241 agaattaatc gacattttaa gcaatcgtta agcggtagag aaaatatcat tattctagaa
    72301 aaagatgagc atattattgc aaatattact aaatcactcc gcaaacaagg tttctcagtt
    72361 cgatctactt ctacgattga agaaacgatt catttaattc agaaatatcc ttttgatcta
    72421 gtgattagtg aaatggaact gggaacagaa acggcaatgg atctttgtct ctcattaaaa
    72481 aatgcaaata ctgggtttca accatccata ctagttttaa catctagaga tagcaatgct
    72541 gattataaaa tgattatgaa tatgggagtt tctggaatca tcagtaaacc tttttcgatg
    72601 gatacacttc ttgcatcggt tgagcggtta ctcgctgata gacgaactgc cgcagaaaga
    72661 aaacaacttc taaaatattt atctaagtct tctgcaaaaa ttgcttcaga aaaagcaatt
    72721 ctaacgaacg gaaaaggagg agctagatcg gaaaagaagt atgcttctct attatttaca
    72781 gacattgtaa attttacagc acgttgtgag aaatatccac ctgattctgt agttgaacaa
    72841 attaatacaa tttttgaatt gattacaaag acaattcata agcatgaagg ggatgtggat
    72901 aaatttatgg gtgatgcttg tatggcatac tggatcggag gagaacacaa agtatccgct
    72961 ttaaaattaa tcgatgctgt gcaagagatt cgagataaga ttcaggaagc aaacttaaaa
    73021 tcagagattt taacaaagga tccaatccga attcgctatg gaatgaatac aggggaagtg
    73081 attttatgtg atataggtag ttcagaagca agaattgatt taactgtaat tggggatcat
    73141 gtaaatctag cggctcgctt ggagtctgct tctaagcagt atggagttga atcgctgtta
    73201 agtgaatata catacgcact tgtttctgat gaagtagaag taagagaaat cgataagatt
    73261 aaggtctatg gaaagaatat ccctgtggtt gtatatgaat tgctttgcaa aaaagaacgt
    73321 cttacgatag agcaaagaga attgcttgaa atttataata atgcaaaaag cctttatttc
    73381 attggaaaat tcgcagaagc aaaaaaatat tttatacaag caaacaaatt agaagaaata
    73441 tttccaggaa gacaaacaaa tccatctaag gtttatgcag gtcgatgtga tttcctattg
    73501 aagaatcctc cgaaagaatg ggatggagtc tgggcactta gaaaataaaa cgcttctact
    73561 tgacacaatc tagcatatag aaagtatcaa ggttgtatga aattttcaat tcgaaaacct
    73621 gttttacttt ttactttatt ttttcaaaat tgcattccct atctctatca tgtaagcaca
    73681 gaacaagtaa agattatttt aaatcgaaaa ccaattccag aagtgattga gtctgaatca
    73741 gaagagggga taacgaggag taaactcaaa ttaatacaaa aagtaagaga gtttgctgta
    73801 aaagaaattc atttaaatcc caaaggtgga tttgagtatt ttacaaagtt gaaccgtgaa
    73861 gaagtaggtt ggaatgtaag cgcctcttat cctttgaaat ttgaatctta tacttggtgg
    73921 tttccatttg caggaacagt tccttacaag ggatattttg atttaaaaaa agcaaaagaa
    73981 gaagagcaaa ccttaataga gaaagggttt gatacaagag tgcgtataac ggctgcttat
    74041 tctactctcg gttggttctc agatccagtg atgtctcctc agcttagaat gtcagaagac
    74101 gaattggttt cccttgtaat tcatgaaatg tcacatgcaa ctgtttattt cgcgggagat
    74161 tctaatttca atgaatctta tgcaagtttt gttgaagaaa aaggaacaga acttttttac
    74221 aaaacatttc attccgaaaa ggcaaatgat gttttagaaa aaaggaaaaa ggcaaaagtt
    74281 cagtctgact ttataatctc acaagtaaaa caaacagctt ttcaattgaa agaaatgtat
    74341 gagaaagaag atctaagtga tgaacaaaaa ttagaaaaga aaaagcaaat catagaagat
    74401 tatagagagc ggatactggc aaatagccac ttacttacaa actttaatcc agaaaaattc
    74461 aaaaaaactc ccttgaataa tgaaaacttc attggaattc taagatatca ttctggatca
    74521 aaattatttc aggataaatt tatcgaggtg ggacaagatt ttaaaaagtt tcaccaagca
    74581 atggaggaat taaaagcact atcagtggaa gaaagagctg gacttttaaa agagtgacaa
    74641 atgagagtta gccactaata ttacaaagat gagtcgattt ttaaaaccta gggatttaac
    74701 caccaaagaa tttcattatt tagatagtat ttttaagaag aatagaaaaa ccttaaaaat
    74761 aaccttctat ttattaatcc taatcaattg gattttaaat acacttggta tttttttatt
    74821 tgtaatcgtt tttagagaac ggtcttttct tttactcatt cctactattt tacttttact
    74881 ctttgtaaac ttaggattta atcctttatt taaaatgtgt aaagtaggaa tgaaagataa
    74941 gtttcaacct gataaggtct actttatgga agggatcttt cgattgaaat ggattggttc
    75001 ttggagaccg cgtttaaaat attttataga aaattatgag gtgatagttc ctagcaaatg
    75061 gttgtcagaa ataaaacagg gagaacaaat caccgcagag tattctactt tgcatccaga
    75121 agcaaaatcg aaaaaattat atattttatc cttgaatgat cggtttcgaa tcgattaaaa
    75181 ttttttttct aaagtaatac taccataatc ctgacaagcc tttgattcat aaaaaccatt
    75241 cattttattt ttatttgcaa agaagcgata cttgtccgtg agacaaattt gccatccatt
    75301 cttttcactc ttttctagaa attttagatc ttttaatatg aacaaagtat tcttctcgtt
    75361 cttaaaaccg ctcactgttg ttttacaggt tccacctaac tcacttttcc aaatggcagt
    75421 gccggtcaca ttatttttat gaaagcaaat agataattct aaacttaggt ttggatcttc
    75481 actccccctt ccttttccta tccagatttc gcagttggat ttatttttgt gagacttttg
    75541 ctttgcggat aacggtgagt aaccgtaatt gaataaaatt aaaagaagta gaaagtattt
    75601 catataaata atatcggtat tggaaagatt aatttaaagg ttactgttaa taatacaaaa
    75661 ctctattatt atcatcatct attacataaa gtccaccagc tgaatcaata caaactcccc
    75721 ttggattatt tagactcaga gctgatcttc ctccattgtt tgcagtgtta gatgtaaaac
    75781 tatcattctg gccaaagaca atagttgctg tagtagaatt ttcaggaaaa taaagaactc
    75841 gattgttacc gccatcggct acataaagac cattactcct atcgatttgc acaccaatag
    75901 gagaatctaa actaatgctt gatagaccac cgttatttgc aatactcgtt gtaaagcttc
    75961 cttgctgtcc atatacagtc gaagctgttt tggtattctt tggaaaaaat aatacgcgat
    76021 tgttatataa atcagctaca tacaaattat ttgaggaatc aaaaactata gagaaaggag
    76081 cattgaattt agaagaagtt aatcccgatg aattagttgt aaatagatta tcttgtccat
    76141 agacatcatt tgcagtgctt gaagtatcgg ggaatgaaag aagtctatga ttgtctgtgt
    76201 caaaaagata gagtaggtta tttttatcac gtatggctcc tctaggactg tttaaacttt
    76261 gtgcactgag cccgcctaaa ttacttgttc ctgttgtaaa tccatcttgt tgtccaaaaa
    76321 cttttgtagc agttgttgaa ttattttcat aagccaaaac tcgatcattc gttgtatcag
    76381 caatatagac aaagttattt gcgtcaacgt aaacccccct tggactattg agtgtatcgg
    76441 cagatctacc gccattgttc gcagtattgg aagtaaaact tccgagttgt ccataaactc
    76501 ttgtagcagt tgttgagcct ttgagataaa ataaaaccct attattattt gtatctgcaa
    76561 tatagaggtt atcgtcctta tccacagcgc aatttctagg agaatttaaa ctatctgcag
    76621 aaactcctcc cttattggat gtgcctgttg tgaaatcgcc taactgtccg tagactctac
    76681 tagacgttgt attctttatt tgtaaattca ctgtagcagt taaacttcct gcagtgatag
    76741 tataatcggt cgcacttgca acacttgtcg gaattccttg gattgcaccg tttgaactat
    76801 taatagataa accggaaggc agagaaggag atatactata cgaagaaata ctaccttgga
    76861 cagtcggtgt aatgtttact tttgcattta catagaaaat aagattcgtt ggatattgga
    76921 tagagatact tgagctagtt ggtaaaattt cccgagcaag agagataata cctaatagtt
    76981 ttttatttga attcattgaa caagaaagat aaagaaataa aaaaaatagg aataaaggaa
    77041 ttcgagttcg catagtggaa taataggatg aactgtattt cggtaaagtc aatttttttc
    77101 ttaataattc accttacaaa ctttttaaac atggtgagtt cagcctattg taacattggg
    77161 ttaaaaatgt tagcgtttat attatgtgag attagaaggc tggagacgcc aaacttattt
    77221 tttaaataag actctttttc actttttaaa actatatatg atctgtgatt atcttctcta
    77281 attcttggta tgtgttagct ttattagacg ttaagcgtaa atattcaata tctaatttgt
    77341 ctttatgtaa atagtagagt cgctcagccc attcacagta tgagttagaa ttcctacagt
    77401 gctaatcatt ttttcaaaat tagttttatt ttgctgtttt caggaaaata tcgttaccca
    77461 caattttaaa agattaccac cgatgatacc gataacagaa cgataaaaac gatggattca
    77521 tatatcagtt taattatttc ggttaaatga gtttaaagga ggaatatttg aatccttaga
    77581 ggcttaaacg tgattaaatc gacaaataat gtaaaaaggc aaaaacaatt atatatacta
    77641 accatcggtg cccatcgtct ttatcggtgg tagtcagttt taacatgcct gtgggtaatt
    77701 atatgtttca ggaagggtca taataccact aatttttaga gatgtagcga atctttccct
    77761 cttcttctgc tttcttccaa cgtaataagc aaatttgatt taaaactttt atttcttctg
    77821 aatccgaggt cttctgcgaa aagagggaag tatttattct aattatcgta atcaattgcg
    77881 atgtttatac caaacgccaa aagaaaagtc ccattctaaa aacaaaagat cggtttggtc
    77941 taaacacttc ttagtgttcc cgaacatcag tatgttcggt ataccaaatg ctactccaaa
    78001 aatatccttg cgggaaatta cttttgtcat ttggtatata ttctttcacg catccctaga
    78061 ttcaataatt tggtaaaagg agatttgtat gtataagaaa ttattttttg tatctgttgg
    78121 actttggatt cttgctttaa tcattttagg aaaattcttt attctaggaa atgcacatcc
    78181 ttctgcggat ggaaggaaat acattgaatt atcaaaggaa gaaaaaaaat ttgttttatc
    78241 tgaaatgaga ggattgctta cttctttcaa tggagtgctt gtggggcttt caacaaatga
    78301 taaaaataga atgattgagt cagctagaat tgcaggcacg aaaggacata atacctcaga
    78361 aggaaatcat aaaacaatta tgctgaaact tccaatcgaa tttaaaaaga tgggaatgaa
    78421 tcttcataag gattttgatt tattagctga atcaatagaa aacggagcag aggaaaagga
    78481 aatcattcag aagttagcga atctttccgg tcaatgtgtt caatgccatg ccggctatcg
    78541 attttctgca gaataaaaag gaaaaagaaa ttaaaatgag tgtagtaaaa gaatactgga
    78601 atagagttat caatattgga attgagccga gtttacatga aaaggatgcg aaatacattc
    78661 gcttaaccaa tgcgatttca gttataactt ctgtttggtt attttctaca attccttccc
    78721 tccttcctct ttatccttct agtaaatttc taattttgaa ctcaatttta tttccaatcc
    78781 tatggccttt tagtctttat ttgaatcata aacggtttta tattcttgct aaactttttt
    78841 attcatatac tgcattgatt tgtattagtc taaatgcatt gcaagtagga agggaagcag
    78901 ataatcattt gtttttgctt cttttaagta tatttgcatt ttactcttat cctccaaaac
    78961 agatgaagta tattagtctt gtatcccttt cttcgctctt ttgttttatt gggatagaga
    79021 tttacctaac gtctcatcct cctattttgg aagcgcctcc agaatttttt caaatagcaa
    79081 gactcatttc cttagttgca ctttgtgttt tagtttatgt attgactctt tataattata
    79141 agactctgaa caatgcacag gatttattag aaatagagca caaaaagtca gaaacacttt
    79201 tgttaaatat tcttcctcca gtgatagcgg aaagattaaa agtgagtaat tctgtcattg
    79261 cggagagaac aaatgaagct actattttat ttgcggatgt tgttggtttt actgttttat
    79321 ctcagactat ggagccagaa aagttagtgg cactcttaaa tgatttattt tctgaatttg
    79381 atacaatcac caaatcgtat cacctagaaa aaattaagac aattggagat gcgtatatgg
    79441 ttgcaggagg aataccagag ccgagaaagg atcattgcga agctgttgcg ttatgcgctc
    79501 tagaaatgca agccattatg cacaagggaa tttctaggga tttacaaaaa tttagagttc
    79561 gtattggaat tcacacaggt ccggttgtag ccggagtcat tgggaaaagt aaattcatct
    79621 atgatctttg gggtgatagt gtaaatacag cgagtagaat ggaatctcac ggcatggaag
    79681 ataaaatcca agtatcaaaa gaagtttatt taaaactaaa agacagtttt gaatttgaaa
    79741 ataagcgaga gatacaagtc aaaggaaaag gtctaatgga gacttatttt ttaatcggaa
    79801 agaaagctgg cgtagactaa catctatttg ccttgttcaa tttctcaatg tagaattgca
    79861 tttatatcat ttttatagcg gataataatt attaaccgtt tcaggggaaa cttagttatt
    79921 gaaaaatgtt gaatatagag aactgctctt gagataatag taaattctat atagagaatt
    79981 tcattagctt ttatgtttta tagatttagc tgatgagtct gtaaacgctt atatatttag
    80041 aatagagaag aatgttattg ttttgaaatc tttcaaatta cattatactt tttatatctt
    80101 gttttctatc cactctgaat caataaccat ttgtgtcata cttccagagt ttgacgttag
    80161 tgattgttcc actcgttcct atatcagaac aatttatagt attggtagaa cttgtagtat
    80221 ctgctttaat taattttgta gtatttgatg aatcagaact gtgtccttgg taagctccaa
    80281 tgtttggagt aaaaatacac tcttcattgg attcacaaaa accattttca ttgccaactc
    80341 catctgcaaa aacttcaaca gcatttcgta aaaatgtagt aatacaagta gttccattca
    80401 gctttgagcc tttataattt gcatcacaat aggcttggtt aggtgcagtg gagtcagacc
    80461 atagatgagt atcaatgatt gatccattag ggcaggagtt aacgtttcgt ataatagtat
    80521 ctgtattttt tagagataaa tcccaaatag aacatgttcc tgttgtgcat gagcctgtta
    80581 aagctgaggt aggaaatgtt ccttgtttgc cccaacctct aaattgatta tcgaaattaa
    80641 accaatccgt tatacttgca aaagattgag aaccgcttgt atttgataag ttctttgtat
    80701 cacttgtgac tcctacaaaa ctacttgcta aggtaatatt ggttactgtt gttggacttg
    80761 tttcactagg actaatttta gaacaagaag cattgattcc gggtgaggta gcagcagtaa
    80821 ctgtgcaagt tccagaccca ctcactttta caatcccata aaataaagaa cttacagtat
    80881 ttgattcgct gtattgactt gtgtttccac tgtgggcagc aactgtattt ataaatttgg
    80941 gactatttac aattgaatta tctatagcgc ttgtcgttcc tccagtattg gcagcagcta
    81001 aattcattga cgttgattga aaattgtttg cccaagcaaa agctgaactt gtgctgttta
    81061 cgcttgttat atttgcaaaa aaattattat tataagaacc attgatagta aaacctagac
    81121 tgcctgagtt cagtgaaata gaatctaaaa atatattgtc actacttgaa acggttaaat
    81181 tgaaactaga agaattatac atttgtatat ttttaagaat atttttagat tggactaaga
    81241 taagaaatcc acctcctgta tttgttcctc ctacgttatg gtatagactt gaggaaccaa
    81301 cagaataaat ggttgtagcg ccactagttc tagctctaaa attacgaatc atattaaatt
    81361 tacttccact ggaaatcgac aaaactatat tagctgcact ggaatcaaac tttccttcaa
    81421 cccaaagaaa gctatatgtg cctaaggcaa gggaaggaag tccacttgaa agttttgtta
    81481 tttcgggctg cgctactaat ccaatattat tctgcgtaaa cgtgtaggta gaagccgaag
    81541 atgtattatt tattatgtaa attgtttttg ctgaactaag tgaacctcct gtgttgttta
    81601 gaacgatagg attgttccac cactgactag cattcgtaat taaaaatacg ttattgttaa
    81661 gagatatagt aactgaatta tttttccaac tccctgacga agtaaaatca attaaatttc
    81721 ctaaaccttt gtctgttttt aatcctgtag aaactatata aacagaatta tctgaggaaa
    81781 caacgcattt ccagttgaat gaagaaagta aatccgttgc tgcaattcca tcacaggaag
    81841 agatattcgg tatacttatt tttttaaatt ctgcagcatg aatacaggca ttgtaaaaac
    81901 ctgtctcaga gccagtgcag gctgttcccg atgcgttaat tggactagtt ccatctgatt
    81961 ttacataatc taaccaataa gatccagtgc tgtaaagact tgttggggtt gcagaggcac
    82021 agataatatt tacattcgta atattattta ccactgttcc cttggcattg tcgatggaac
    82081 aagaaaatcc ggaaggatgc gttttgggta gaatagaata agatgttcca gaggtaagcc
    82141 cactaaatga atattgcgtt agtggagcag taagtgttaa atcatccttt ccattatttt
    82201 gtaaaattaa ggttccattt gtaaatccag taatggtccc accgactgaa tatccttggg
    82261 attggttagc ccaaactagg ctaaaaatca atcctacagg actactaaag tcaaaggcag
    82321 atttttttac ttttaaacaa ttggtgaaaa gaaagcataa taatagaatt tgaatttgtt
    82381 tcataggcaa tgtaattttt tatgaagttt aattcatagc tcatctataa atcttattat
    82441 attaattact actagttatt aatataacta gtaaagctat acgaattttc tatttgcttg
    82501 tagaaatcgt tttaatttcc ttctttaatg aattcaccga atatccaacc ttggttatcc
    82561 ttagagttac atctactata ccaaccacca tttacagtca cataatacca atagttttcc
    82621 caatttttta ccttttgctt ttcgttcgtc ttaaaaagta aaattaggtt tgtatttttg
    82681 gaaacataag gaatccagtc tggtgccatt tcagagatac aagaaaagga ctttgaattt
    82741 acatctggtt tttctctaag ttttgcatca tctgtggtta ctcctgttat gccactcaga
    82801 gctgtgacag aaactccttc gaattgaact tgtgctcccg attgaatttt ttcggataca
    82861 ttaaagaata cagtttttcc acagtctagg tagaataagg ataatacagg attcggaact
    82921 tttttgtatt cacaagttct ttctggttca tcgggttgac aatctttagt ttgttgaatt
    82981 ccagttttga gtaaaactct tttgctatcg agtaattcat attcaccaat ccatctttga
    83041 ccacagcctt ctccatagaa ttcaattgtg tatttattat ttgcatcgaa tattaagaaa
    83101 tttcccatat tagaaaattc attttctaac caatgaatag acgttagttt ttcttttgta
    83161 agtgtatcat tgggagaatt aaccctgtta catgctgtaa aggcgatgaa tggaattaga
    83221 aagattaaaa gtttcattga attgaatcct agcagaataa tatattttat ttacaactta
    83281 cctaacgcaa acatcatcta aataaatcga tactcaaact caattctttc ttttttagat
    83341 gctaatagta aaagcgtaag gcgaattata ataagagttc atagaaaacg cattttaata
    83401 tttgtataga attattttat aaataaattc gaccagatga aatttataga atttgaatgc
    83461 atcgaatttc cgcagatcaa ttggaaacgt tgccttctca ccaaacatcg cttatacaaa
    83521 ccattctcaa atcattccca tttctttgca aaattttacg aaaggtaaaa ctctccctgt
    83581 tttattggat ccaaaatctt tagtccccat gtggacttgg aagaattctg gaatttctgt
    83641 tcttgcttga aaataagaaa tgtgagggct taaagctttt tcgccggttt tgcattccac
    83701 agcaaagaga ggttttttat tttttaaaac aacaaagtct atttctcttc cgtctgtatc
    83761 tcgtaagtat cgaatctcca tcgggtaacc ttctgtgtct gttataaaat gacaatactt
    83821 aagcaaatga ctcgcgacta agttttcgaa tctaaatcca tattcttcta cttgtgacca
    83881 atcccataga tataattttt ttagttttct aactgctctt attttaggtg agccaaaggg
    83941 agctatcgta taacagtaat ataacgtaga taagatttcg atccatctgt ccactgtcgg
    84001 ttgtgatact tgcaaatctt cactgatact tttcaaagaa agaggagagc caactcttgc
    84061 tggaagcatt tcagcgagta gtaacatgga agaaatatcc tttatatttt ccaaatctcg
    84121 aatatcttct gaggcaactt taatcattcg atcattttgc caaatgcgat gctccttttc
    84181 attttgcgaa aacagcggtt caggaaatcc tccaaacttg agtaaaatat ctaaatcatt
    84241 tttgtttgga ttcttattca tttccatcac agaaaaggga tgaagtctaa agtatcgata
    84301 acgtccgaga agagagtccc cgccttttcg aaagtaatcc agtcttgcag agcctgtgat
    84361 tataaattga ttctttgtat gatatttatc gaataatcct ttgattaaat ttctccactt
    84421 cgaatattta tgcaattcat ccagaactag aatcttattt tcctgaagag gaaactgatc
    84481 tttcaaaatt tgaattctat cagtgttgcg atcccaattc aaataagcag gattttcaat
    84541 tgagggagat ttcaaaaatt gtaatgatag tgttgtttta ccaacttgcc ttggacctcc
    84601 taaaaaaacc attttcttat tgagagactc ttgcatatgc ggaattaggt atctgagtag
    84661 tttcatatgt atgttaaaat actcatattg gagaatttta tttaaactca atgcaatgag
    84721 aactgaaaca aaaatttatt tagaatcata aattttgcat catatttggc aaatgataaa
    84781 agtaatttct cagaatgatt ttatttgggt agcatttgtt atatcgaaca tacagacgtg
    84841 caagaactct gtgaagtgtt taggtgaaac gcacttttta tttttaaaaa gggatctcta
    84901 ttttaatgtt tggtataatt tgatttggat aatacacaaa cttttgggca aactgattgt
    84961 tttacaaaca aataaaagaa ataaatttcc aaaaatttag agtcttatct gaaagggcta
    85021 aagtgtaata cagtttgccg ataagaatag aattattagg aaaggaaaat atgaagtata
    85081 tactcatttt gatcgcttgg ttatttttta taaatatatt tgcagaggaa aaaacaaaac
    85141 tagatgaagc agaaatcaaa gattcagaaa agatagattt ccaaaacaag actagaaaga
    85201 aagctgcatc ggaaacaaaa cttttacacg aatccattgg aaaaacttta ggatctatga
    85261 tttccaaaga tccaaatgaa atgcattccc aagagggagt tcgagtggtt cgtgtgagca
    85321 cagcggagaa aaataaaatc ggggcggatc ttcttttttt agatgcgaat actaagtttg
    85381 gtcacatcaa ctcaattcaa agagtattat ctgggtatat tcaaactgca ttcagttata
    85441 ataatgcaaa agcagatatc ttagcgactt atgttttgta ttacaacgga atgcatagag
    85501 gagacgttcg cttctttgaa tccaagtata ctaacgcttt attaaagaaa ttgaatagtc
    85561 ctaatattgg aattgcatta agttataaag attggccagg taaaacagaa ctcatcatac
    85621 ccattgaaaa aaatatatta aaagaatcgc agagagatgt tacattagat gaattagaaa
    85681 acgaagtgaa caaaatcatt caagataaaa agaatgggag tgaagataga aataaatttg
    85741 cagagctaag aaaagaaaaa attgcagaag agttaaaaga aataaatcaa aaaatagatg
    85801 agcttgaaaa tttagaaaat gaaatcgaag caaaacaaag aagtattgtg gagcgtttag
    85861 cggagctgaa aaaagatcct gacaaaaaca aagacgaaat tactaaattg gaaaaggaat
    85921 atgagaattt agaaaaacaa aaaaatgaaa ttagaaaacg aaaagaggat ctaattgaca
    85981 gagaagagaa gctaacaggt gttaaatcca agagaagaat tccaaaagaa attcctcagc
    86041 caaaagaaga aaaaaaacta gaaccaaaag caattataga tccaagacta ttagagcctg
    86101 aatattcttc tcatgtggta gatggaaaat tagtttttat aaaaccgatt acagatatcg
    86161 acagtcattg tgtaaatgga attcatttac ttgatccatt gaaagaagat tttgtttata
    86221 caagtgagta taataaaatt tgtggaaaaa cgtttaaagt tctaggagat aatattttag
    86281 tgattggatt tttggataag aaagaacaaa tcaaattgat tttattaaaa aagaacgatt
    86341 taaaagtagt tgggaattca gatgtgaatg tatatcataa aactccaatc gaaatccgag
    86401 gagatttttt atatgcaatc gaaaatttag atgggaagtt ttatttgact cgctttgatc
    86461 tcaatttaaa aagtttaact cgttctaaag aagaagtaaa tcctgattca gtcattacct
    86521 tttatgataa caaaatatat gtatcaggca aaaaagaaaa cggacaaata gagtttaaag
    86581 tatttaaaaa ggaagattta agttttataa aaaagaccca aacttaggtt tgggtctttt
    86641 ttggtgcggt aagcaatgga attttttata ttgcgcgatg gaatcaattg gagaacagga
    86701 actatgtcaa gtttgaacaa atgaagtaat ctggtatctt gaaattaaaa tgcccttgac
    86761 agatttgtaa aagtagatca atatattgac ccatgatttc aacttttgcg aaaaacatgt
    86821 tacaaaaaat actaaatata ctgattttat tgggtttatt ctggatactt tctaattgta
    86881 gtcggcttcc gaaggataat tcaaatttag cctttgtttc ttttttggga aagttagtaa
    86941 atgtgaatga ctctgtaatt tcttctccgg attctgctcc acaagttgtc tccactgatt
    87001 ttgtagcaag tattaacgca acagctccta cttcacaagt tgttatttct aagacacaaa
    87061 ctcttggggt tacttttgat aagccgatga gtgcttctga ctgtgttgca acaatgaatg
    87121 gagttgcaat ccaaactacc aatacagctt cttccgattt aaaaacgatt ttttttaaac
    87181 cagttaccgg ttggacagtg gatatgataa atggagttac gattaacttg agcagtgcat
    87241 gtaaaagctc cactggatct gcttatgttc cgggcgctgg tgttctgatt tttgtagcag
    87301 gttctacgat ttatgtagat ggaacagcag gtagtgatac caatcctgga actataacca
    87361 gtccacttaa aacaatttcg ataggcgtta caactatcgc ttctagttgt agtgtatctg
    87421 acccttgtgc aatccttgta aaaggtggaa tttatacgat tagttctagt atcactattc
    87481 caagtaattt agcaattttt gggggctatg atcctgctga ttggaaagtt agaagagcgg
    87541 ataagacgag tttgcctccg tatgatactg tattaaatga ttcctctact ggagtttctg
    87601 ggattataag taatccatat tctactttaa acttttcgaa tacgactgga atcaatatta
    87661 tagatggttt gattataaat ggaaatatta cagcaactgc aagcagtttc gtttctccta
    87721 ttgcaattac gaatttacaa actggagcaa cactttcgat tcgaaatagt atactaaaag
    87781 atgtgagtag ttcaccgagc gttacttctg cgggtcttat tgcttataat aattctggta
    87841 taatcaatgt aatcaatagt caagtgttag gatccacagc acttgcggca agcgaaagac
    87901 atgcgcttta ttataacatt tccaatacga atagtgtgat caccgtgacc aactctatct
    87961 tagatgctgg ctattcaaac ataaactcaa gtggcttttt tatggctgct tctagtaatg
    88021 gaaatattaa tctatcaaat aatgtgataa atggagctat ttgtaatgtc tgcaattcaa
    88081 tcggtattac tgctaatttt gctacaccga atggaatgac aatttctcaa aacacaatta
    88141 ctactcgaac aggcaataat tctattggaa tcaattttac ctctgggacg ggattgtcta
    88201 ttttaaataa tataattaca acaggaactg ccacaaacca atcgattggt attaatttag
    88261 tcagcaatac ttctacacta actgcgtcta ataatacatt atccgctgga agttgcactg
    88321 cagctggatg tgttgctgca ggaatttatt cttctacttc ccttgctaca ttgaatgtaa
    88381 attcgaatac gatatccgct ggatcttgta gtggtagtgc ttgctttacg tatggaatct
    88441 atttagggaa ttctgccact gctacgaaca caactatttc aggaaatact cttagtttgg
    88501 gtagtgcgac gacaggtgga tcgtatggaa tgtatttcac ttctacttca gccaatcaaa
    88561 gtcataccat ttctggaaat acttttactg gaggaactgc gagtggagga caaatcagtg
    88621 gaatatatag agcaaattca tcggctggtg cgacggtaac aatatcgaat aacgttgtga
    88681 caactgggaa ttgttctgga agttgcatca accgagcatt tgacattcaa aatgtaagtg
    88741 gaacaacaac tatttcagga aatacagtaa cggctggttt aggagtagga caatccattg
    88801 gaatcgaatt aaataatagt tcttttactg tatctaacaa cacaatcact gctggcactt
    88861 gcacgggagg tagttgcatt tcgagaggaa tcaattcagg atttggaagt ccggttacga
    88921 taacaggaaa tacagtaaca gccgggacat gttcgggagc tacttgcaat caaacaggga
    88981 ttaatctcgc aataggatct aaccctgtta tcactctttc aaataatacg gttgatgcag
    89041 gtactccgag tgtaaataca acatctagat tagctcttaa tttgactaat tgggcaaatg
    89101 gaacgagtat tcagaggaat acatttatta accgtatagg agcgggaatt cctacgacag
    89161 taaggttagg tgcaatggca tcttctctta ccttttgtag taacgtccta gttggcggtg
    89221 gaacgaatag tgcagtaact gccactactt tggaaattgg tttgattaca ggagtaagta
    89281 aattcaaagg gaatacaatc attggagcga gtgcgacatc tggcactgta tatcctgtaa
    89341 attttctaac aacaggaact tataccaact tagctttaga tcaaaacatt atagcgggtg
    89401 atcctgcttc ctccgcttac acaacaggaa ttagagaaag tggtgcagta atctattcct
    89461 ttttgagtgc aaataatctc tcttgtgttt ctactctata caacgaaaat ggaatcattc
    89521 gaaatttctt atgcacagca acacagatag gaaatttttc aagtgctgat gcactttgta
    89581 gtagtggaaa tttagcaaat ccaactggtg ttaacaatac aaacttactt cctgtatttg
    89641 taaatccatc tattaacgac tatcatttga atgcagcaac acctgtcgga attacacaag
    89701 caatgacagc agcagagctt tctactttca caacggattg tgggaactcc ttggatagag
    89761 atggaaatac tagaacagca aattcttcca ttggggcgta tagatagaac attttggttt
    89821 tggatagatt catctctttc aagcgttagc gatcaactgg atgactgtgg ttctatctat
    89881 tctattgcga ccctaagaag aaacattggg ctttagccag aaaggtgggt cgccgaccaa
    89941 atcagaatag ttcacagtca ggaaggcgag cgcggcgtta cctaaccccg actttctatt
    90001 ttttggttta tctatccgat agaatcccat tccctaaata tcttgtgata gaacactgaa
    90061 tttagtttta aatcattccg gaaggggcaa cgtgtttgaa tcttacaata tggaaaaaaa
    90121 cggggttagg tcacgcaacg cccaaaggaa acgttgtaaa ttagacaaag tgtactaaaa
    90181 aagacttttc taggatacgg ggttcgagat taagttaaat taaagtcaaa aatatttatt
    90241 ttaagaggcg tttatgaaag ttgtcgtgct tgtgaaacag gttcctgaca cagaaaccaa
    90301 tattaaactc ggtgacaaat ctatcaatga agcaggaatc aagtggatca tttctcctta
    90361 tgatgaattt gcaatcgaag aaggcattaa gctgagagaa aaaaacggcg gagaagtgat
    90421 tgctgtttcg gtaggtcctg atagagtgca agaagttcta agaactgctt acgctatggg
    90481 tgttgataaa gcagttcata tcaaagtgga caattacaat acatttgatt ctctttttac
    90541 ttcccaactt atcgcaaatt ttgcaaaagc agaatcagca gatattatct tagctggaag
    90601 acaatcgatc gatacagata gttctcaagt ccctgttcaa attgcggaag aacttggtgt
    90661 tggacacgta gttctcgcta ttaaaattga aatcaacgga agtagtgcaa aaatcactcg
    90721 cgaagtagaa ggtggaacag caactgttga aacttctcta ccagtcgttg tgactgctac
    90781 caaaggatta aatgaaccac gttatccatc tttaaaaggt atcatgtctg caaagaaaaa
    90841 accaatcgat gtgaagcaag ctgctgattt aggagcacct gcaagcaaaa tcgaaatcgt
    90901 tggtcttgag ccacctccac cacgtatcgc tggacgtaaa ttagaagcag cagatgcagc
    90961 aggttttgca tctcagttag tgaaagctct cagagaagaa gcgaaggtta tataaggagt
    91021 ccgatatgtc aaatgtattt gttgttggtg aaattaaaga cggtgaatta aaaaagatct
    91081 ctaaggaact aacgtctgct ggtcgtaaga tcgcagatag ccttggggga caagtagaag
    91141 tattgcttat tggagccggt gcagaaaaat ttgctggtga tttaggtgca gttggagcag
    91201 ataaagtttt aaccgcaagt gctggagatt ttaatgctga ggcatacgca aatatcatca
    91261 gtgaagttgt taaatctaaa aaaccttctg tagtattaat gccgcacact ggttatggaa
    91321 aagattattc tccaagagta gctgttaaaa ttggagcagg aatcgttgct gacgtagttg
    91381 gtctaacggt tgacggtgga aaagtagtag caaagaaacc tatttattct ggaaaagcat
    91441 acgcaaatat caaagttaca actgatatcc aagcatttac agttcgtcca aactcacaag
    91501 aagttggatc taaagctgga gcaggtgcaa cagaagctgt atccgctaac gctggcgatg
    91561 taaaagttaa aattactgga acagaattaa caggtggaag caaaattcaa ctcacagaag
    91621 cttctatcat cgtatccggt ggtcgtggaa tgaaaggtcc agagaatttc ccaatgttac
    91681 aatctctagc agatctttta ggtgcagcgt taggtgcatc tcgtgcggct gttgactctg
    91741 gttggatttc tcatagccac caagttggtc aaactggaaa aactgtttct ccaaattgtt
    91801 atattgcttg tggtatttct ggatctattc agcacatggc tggtatggga tcttctaaat
    91861 acatcgttgc aattaacaaa gatccagaag ctcctatctt taaggttgct acctacggtg
    91921 tagtggctga cctttttgaa gtagttcctg ccttaacctc tgaattcaaa aaagttctag
    91981 gttaattttc tctcgggcgt ttcggcgcaa cctactgtag agacgcacga ccgtgcgtcc
    92041 ctacgaaaaa atcgccgccg cgctctacat tcccgtcaat aaattgctgg ttatttacca
    92101 aagcaatgtt ctctcgaaat cgcaatttat tgacgacatt tcgatcgcta acgcggaaac
    92161 cttcttgaca aatgacactc caagtgtcac attaaacaga tgacctccat aacaatgcta
    92221 gacctgcgaa agaactcaaa tgttctttta gagaaactaa aaagaaaaga aaaaatactt
    92281 ctttcatttc gtggaaaacc tgttgctacg attcatccta tttctcagga aagtgattgg
    92341 gagtctgatt cttttttaca atttctaaat tcgattccta agaatcccag caaaaaagtt
    92401 aagactaaaa tttccaatga tgaaattgat tcattagttt atggcatcaa ataaagattc
    92461 taagtatcct ttttgggaca cgtctgggtg gttttctttg ttagtcgcat cagaatcaaa
    92521 tcattccaaa gtccgaaaca tatcagatga acttacgaaa aataaaatac ccttttttac
    92581 ctctgacctt gtgatacaag aaactatgac tctcctgatg gcacggaaag aaaccgaaaa
    92641 agcaaaacaa ttttggtcaa gtcttgttga ttcgaaagta gttcgtatcg agcgaatcga
    92701 cgaaacacga tttcaaaaat caggagaata tttttctaaa catattgacc aaggctactc
    92761 ctttgtagat gcttcctctt ttattttgat gaaagaattt aaaattacaa aggctgttac
    92821 tacagataaa cattttagga aggctgggtt tataaagttg ttggcttagt atgttttagt
    92881 ttctaacgca aactctcatt ttccgccaga agctctttca gtttttctcc cttcaaaatc
    92941 aaaataggtt gatgattcta aattgttttg tataaatcct tctactaata acgggttgtt
    93001 tttctactta gtaggattgc ggtcaatgtc attaagttaa gactgtcacc cccgaaattt
    93061 tctatcatgg gtctctcgaa atcgcaattt attgacgaca tttcgatcgc tgacgcgctt
    93121 aagttgatag actaagaaga aattgtaatc tctttttgaa aaggaatagt tatctctagg
    93181 aagtaatgct tatgaaagat ttatcttatg taaaaacaaa actgatacaa gaaaaacagt
    93241 ttattcaaac taaccctaaa attaaagacc ttggtatttt gggctcactc acacatcttg
    93301 cggctaaatg gtatttaacg cttcagatat tactctttac tagctttgtt ctaactgaat
    93361 gcgccttaca agggttattc tctttcggct cttgaaattt ttcccgattt tgtaagagat
    93421 tcgattcatt ctttagcaat tcaatagtga ctcgaaaacc atagagtgat tttagatctc
    93481 ttaatattgt aaggaaatct tatttctact ttattcattc ttaagtttcc ttaaaattgt
    93541 gctattatgc tttttgtaaa tggaacatct gcatgagatt gaagaataca ctaagtcgaa
    93601 aggtaaaaag ccaagaggaa atttgtcgta aaggctggaa ttaatctttc gggttagcca
    93661 aatcttattc aactctaaac gcttgacaat ttctacatat atatattagt atatatatac
    93721 agtctgttgc gaaatgttaa agaattaaaa gtcagccttt aaaaataaa
//
LOCUS       DAOQOS010000004        91135 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_74_length_91135_cov_6.780654, whole genome shotgun sequence.
ACCESSION   DAOQOS010000004 DAOQOS010000000
VERSION     DAOQOS010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 91135)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 91135)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..91135
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74_length_91135_cov_6.780654"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1269
                     /locus_tag="PLF66_01420"
     CDS             <1..1269
                     /locus_tag="PLF66_01420"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HMZ62720.1"
                     /translation="KNLPILSSLWIFTIGFIYVSLTVTLMVSSPSLIQADTLTGEKLS
                     LNLVLTFFPSLLFVHAIIPALIGYFLVNDFTLEVRETVHENFNITLPARHRKIGLTLA
                     ILFGLLGLYPSLMIIIDLMSLSNSPNSYTQFMKLNPIETILVDCCIVFISMALLIFLI
                     KRSFTKPIDRLLGKMNQVREGDYTTQAAILSDDEIGIFAGEFNRMVKGLEEREFIRNT
                     FGKYMTHDVANVILKNKINLEGEMRICTILVTDIAGFTSISEKLSPVDNVRMLNEYFS
                     VLVKIIQENKGVVNKFIGDSIFALFNVPLNDPDHAMNAINAAREIRNMTTKHKFGNNY
                     ELSTRIGINTGMVLAGNIGSKERMEYTVIGDDVNIAARLEQLNKELKTYILIGENTYS
                     LIKDKIECAFIKNVQLKGKENSIKVYTIHH"
     gene            1579..2184
                     /locus_tag="PLF66_01425"
     CDS             1579..2184
                     /locus_tag="PLF66_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HMZ62721.1"
                     /translation="MQNRILILFAHPRFENSRVHKALTHSIPKSNSITFHDLYDLYPN
                     FNIDIEIEKKLLLDHNIIIWQHPFYWYSCPPLLKQWIDMVLEFGWAYGPGGNALQGKF
                     IFNTISTGGANEAYQENGRNRFTIKQLLAPFDQTSYLCKLKYLPPFLVQGTHRLSDQE
                     VKDKSDEYATLLNTLTEANLADINFDSFANLNQWILQYKKV"
     gene            2186..4075
                     /locus_tag="PLF66_01430"
     CDS             2186..4075
                     /locus_tag="PLF66_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012389095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation:proton antiporter-2 (CPA2)
                     family protein"
                     /protein_id="HMZ62722.1"
                     /translation="MSHDFLIQAIVYLTAAILCVPIAKKIGMGSVLGYLTAGILIGPF
                     VLGFVGQEGKDVMHIAEFGVVMMLFLIGLELEPEKFWKLRKVIVGMGLSQVVITSLIL
                     FLLGLLAGFTWRVSVAISLSLAMSSTAIVLQSLKEKGMMNTSAGQGAFSILLFQDISV
                     IPILAIIPLLAVSQVTQDSSHNSHSLLENAPAWLQTLMVLGAVGLVALSGRFLIVPLL
                     HQVAKTRLRELFTASSLLIVVAIAYLMGLVGLSPALGTFLAGVVLANSEFRHELESDL
                     EPFKGLLLGLFFIGVGASIDFHIISENPFRILIYVLVILLIKTVVLFGIGKIFQMSLD
                     QNLFYSLALSQVGEFAFVLFSFIGQLGILDAASNGIMMAVTAITMTLTPMILLTYEKL
                     IQPKLVKNKEEEKEADSIEENNSVILVGFAHFGSTIGRFLRANGVNATILDNDSDRVE
                     LLRKMGFKVYFGDATRLDLLESAGAEHAKIFVSAISSPETNYAIGEMLKKHFPNLSFF
                     VRAQDRFAAYELIDMGFTKVYRENLDTAIRVGIDILKEIGFRSYTATRSGQNFLKYDE
                     DALIGLAKDRHDSKKYISSVREQIEQIEKLLSTDISQKANLADHAWDSDPLRTAANEM
                     KPLNQ"
     gene            complement(4065..4907)
                     /locus_tag="PLF66_01435"
     CDS             complement(4065..4907)
                     /locus_tag="PLF66_01435"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HMZ62723.1"
                     /translation="MNQYENIAKVLEYVFESNQEQADLNNLSSFTQLSSDKIQEIFRE
                     WGGVSFYEFINLLSKTSIKQVLNNENTLFENLNLKLDSKNNISHDMVMQIVPMSLDEY
                     KNEGENLTIHYSFQKSVFGEYMLASTDLGICSLNYNENLDKTLNELNEIWKNAKFIKE
                     MDLEQEKMVRFMNGENLSDQIKLHIKGTDFQIKIWETLLKIPKTKLVTYSQLGKDLNL
                     ANPTKAARAIGTAVGSNPIAYAIPCHRVIQNTGIIGEYRWSRIRKLAMIGYEASHCLK
                     SSID"
     gene            5030..5890
                     /locus_tag="PLF66_01440"
     CDS             5030..5890
                     /locus_tag="PLF66_01440"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HMZ62724.1"
                     /translation="MQRKLILSFLIFIGFIGSAYFITYNRVKKEAMKLPDNSPSICST
                     PNNALPRVVLVGDSITHGKVSANYVSILENNFQNQYQFINAGINSNLAYNVLQRLDEI
                     IQCKPTFITILIGTNDVNSTLNTKNQSRYISDMNLPRTPDLNWYEENLRKIIERLQKE
                     TSAKIAVLSLPVIGEDLNSTANQRVKTYSAVVKILANEYNIKYLPLNEKQNEFLAKQD
                     NISQSCESNSDNLMITAIASHFLFKKSWDEISETNKFKLVTDCLHFNHLGASFISDLI
                     ADFLKKSPSK"
     gene            complement(6699..7433)
                     /locus_tag="PLF66_01445"
     CDS             complement(6699..7433)
                     /locus_tag="PLF66_01445"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HMZ62725.1"
                     /translation="MVVAQPYSKFSEIYDHVMGHIDFKRWALFILKSGFPNSSPERAL
                     DLGCGTGSLFQYFPDSTYKVGLDISNEMIEVAKRNYPHIEFTVGDIRKFQFSNSFDLI
                     TCNHDTLNYLTTTNELSTHFKTIYQNLSDNGLYFFDVSSEKNLIQNFHDRIFRETIGD
                     TFFIWENMYDQKNKEITSSLYFGTNDQDGFKEFREVHKQRFYSNEEIISELEKNGFKV
                     LKIGSDYKRWSYDKNCSLVNFKVRKK"
     gene            complement(7442..8188)
                     /gene="surE"
                     /locus_tag="PLF66_01450"
     CDS             complement(7442..8188)
                     /gene="surE"
                     /locus_tag="PLF66_01450"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001023254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0008254 - 3'-nucleotidase activity
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HMZ62726.1"
                     /translation="MNILLTNDDGIESKGIHALRKTLSKEHSVFLISPSRERSATSQA
                     LTIFHRMHVEKIEDSVYTVDGFPTDCVNIGLFGNIFPEIDMVISGINRGVNMGHDVHY
                     SGTVGAARHGAVHGKYSLAVSSGNREDGYEYSKESEYIRRFISNKIDILSKGVVYNIN
                     FPLEYSDDLEEIKLSRLGQRTYSDRYEVAHIKDNIYHYFLALTELGYVNSHGSDFESF
                     YKGYVTLTPITLYTTDKDEFHKLQEHFYKS"
     gene            complement(8203..8784)
                     /locus_tag="PLF66_01455"
     CDS             complement(8203..8784)
                     /locus_tag="PLF66_01455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62727.1"
                     /translation="MNLYIQMISELFLDRDEFLRLYSPMNKTVKFFEFFCILSGSFSL
                     SVATLFASSSINSNYVGTLFLLTIGHYFFLSIFSNYISYSIDIKAKDLLKSGDLPTLI
                     ASARSLNIIYIFSCPIAVVFTFIGMPSVSIFMVVIGVSVSAYIWLFSKYANDVYSIGS
                     FRSFRIALSAYFFVLYFPILLISYFLVNFTAIL"
     gene            complement(8794..9750)
                     /gene="sppA"
                     /locus_tag="PLF66_01460"
     CDS             complement(8794..9750)
                     /gene="sppA"
                     /locus_tag="PLF66_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000440816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HMZ62728.1"
                     /translation="MTTNRTLLLFILIFTFTSTVLAMIHISIQAKSKIARTGNVNVFK
                     NTTDSALLLKIEGEIHSGKSTYQSTGADTVLAKLREIEDSNEIKGVLVEVNSPGGTVG
                     ASQEIFDELIYLKEKKNKKIVVSMKDIAASGGYYIASAADYIFTEAGTITGSIGVITI
                     SPNISGLLKKYSVEMRVYKAGKYKDILSMFKDATEEEESIIDSMLSDTYARFISDVAK
                     GRKKKVSQIEKLAEGKIYSGESAVKNKLVDEIGGRREALAKLSELCGVTNLNLLEDEE
                     NPFDRFFNIMGTKLGVNSNPIQIGSFKSPILLILPAALKMNN"
     gene            9854..10870
                     /locus_tag="PLF66_01465"
     CDS             9854..10870
                     /locus_tag="PLF66_01465"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HMZ62729.1"
                     /translation="MKKVFISGITGQDGAWLAKLLLSKGYKIYGGVRDKDSLIDWRLK
                     QLGILESIEFIKINLLDPASVENCIRELRPDEFYNLAAQSSVAESFHSPLETSLVDGL
                     GVLYILESIQKHSPITKFFQAGSSEIFGNAQESPQTEETPIKPSSPYAIAKAYAHWMN
                     INYRKVFGLYCVNGILFGHESELRGKEFFSRKVSLHVANWYHGNRQILEVGNIDIEKD
                     WGYAEEFVLGMYLSLQLDKPEDFIFATGTKTKVKNFIDSAYGEISVSLQWEGEGDNQK
                     AYDKKTGELLVKINPKFYRPIEVKTACGNPQKAKEILKWEPKIYYHKIAELMVRSDIQ
                     NLKK"
     gene            11214..13094
                     /locus_tag="PLF66_01470"
     CDS             11214..13094
                     /locus_tag="PLF66_01470"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HMZ62730.1"
                     /translation="MYKIPSRKPYYILLISFIVFSILFFYYKDLYKEFIIEKIYTSIN
                     FKIEFKESSLSFFPSPGIQLSDVRINNIEEYNKYNAEINKMGFYFSWKILIGIIELNS
                     IHIKSGKLELSPLLKEPNLNSKNSMDPKYIQKLIGYLKLDLIVLDSIEFTLNKNETRY
                     EFYIKYLAITNDHLREVGLAFDISHNNGRFISDTKVLFINSDYSFQSLQIESKIKFSN
                     FILKPFKDYYSLVRGANFDNTSLSGQLNIYKPLLKNEFNFKTDINISGLQFSGAPIYP
                     IIGVSSELMYSIENQQIYFSNIRVNYEKGAIANASGNLSFTKDIYLNLNINGEYADIY
                     KVVYIIVRSLDIRISSNLNFYSHMKINCYKAVFDLYEFKNANLDLDIINSLVKIKVNH
                     ADTIHGHISGDGSIQASSNTSYNFNVILKDINTEEWIKKYTTNPYIKGALSANLSLTS
                     NGNNLENFLENLSAKGKLEVKKGELLGYANILKPVFSLGKLVNILGPRGKNTEFQSLI
                     CDFSIYRKNISVQNLKMVGVGIDAHGSGNITLDRKIDFRIYAGLGGIAGKALYIPIIY
                     KGIMPDNVSYIDPVWIGSVYVGATLFGGPAGATVGGFAGSAVSEYVNKAWDGLKKIFS
                     RD"
     gene            complement(13105..13578)
                     /locus_tag="PLF66_01475"
     CDS             complement(13105..13578)
                     /locus_tag="PLF66_01475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62731.1"
                     /translation="MKAVFECKSRFNCPVERLFQFHEDRIGFETLVGLDPSVKVIQPP
                     SSISEIGTQAILEVQIFPFIKKIWKAEHIGFEKNHKFIDIQREGPFQYFCHEHRFESD
                     GEYSVLTDHIEFDFFMNSVAKYFIFYKLKFQFLKRHEATAHYLQTDFKNLFCGLI"
     gene            13770..14801
                     /locus_tag="PLF66_01480"
     CDS             13770..14801
                     /locus_tag="PLF66_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020775928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HMZ62732.1"
                     /translation="MKIFHFFLLLLVSCKNLSIPMIADNAPQPHHTENGFQNLDPSAK
                     SQGLGMVLYWKLGNFFKSLPSVEPSDYKIETTLNKGELLQKNSKNLSVTWIGHATVLI
                     QLDGQNILTDPIWSERCSPVSFAGPKRYTAPGISMDHLPPIHIVIISHNHYDHLDLPT
                     LKELETRFKPTFFVGLKNGKLLKEEGIQNVIEMDWWNVQLERDIKITFTPTQHFSGRG
                     LFDREETLWGSYILEGSKETVYFAGDTGYFPQFKEIGKKFPNIDIAILPIGAYEPRWF
                     MKPIHMNPEDSYQAFLDLNAKYLLPMHYNTFVLTDEALDEPLVKTKSFFEANRNKLLD
                     LKIGESKFF"
     gene            14872..15141
                     /locus_tag="PLF66_01485"
     CDS             14872..15141
                     /locus_tag="PLF66_01485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62733.1"
                     /translation="MKKLALVISIVLATAFSTSSLFAKCYGFRDADIKVCVNGDSNAD
                     RKKASETCKKVVGADCGGITGYSGSCTGPKCYDDGGNKKSNIKVD"
     gene            complement(15284..16204)
                     /locus_tag="PLF66_01490"
     CDS             complement(15284..16204)
                     /locus_tag="PLF66_01490"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HMZ62734.1"
                     /translation="MGNYKNLSYFEGKIVPSEKALVSIQTHALQYGTSVFGGIRGYYN
                     SDTDNVYIFRLKDHIKRLLNSAKLVQLQFTIDPAELEKIIIQLVKECGYKENIYLRPF
                     IYTSALQLSPRFHDVKAELAIYILPLNDYLDTKSGLKTMVSSWRRIDDTIIPTMSKAA
                     GGYVNSALAKSEAVQNGCDEAIFLDSRGFVSEGSAENIFIIRDGRLITPSLTSSILEG
                     ITRRSVIDLARKLGIEVIERDVTRSELYVCDEAFFSGTGVQVAWIKEIDRRAIGNGSI
                     GPITKKLQDLFFEMVTAKNSSYKEWLTAVY"
     gene            complement(16365..17501)
                     /locus_tag="PLF66_01495"
     CDS             complement(16365..17501)
                     /locus_tag="PLF66_01495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62735.1"
                     /translation="MKYSLLLSIFILFLNSCIDKENAANDLKDSNQTNKFNLSEKRLE
                     QSNGGSPYFSGIATHPLIEVKEGRIKIILENGYLTLGSKEKVQLVGNENTIQAAVSLV
                     EANNYNSQIEGYIEDVNKNEIIFVPSVSLKHTSVYKMNIRDEKNKKEYSEIIYIAAAN
                     YCDSNSYFSKIPLQQLPKGSEKKIDLSIKENSFKDKEQGQISALAWDHKTSNNFYFSI
                     TNQTQGAKYKILAVYGVYNIPGHECELYLQRETSDLYLTWTDSNYASKVTKITHEDTA
                     IRNYSTQNFYGFSKTYILVKVVSSDNLNITAQDNGILSLQQTDSSYELPVSSNIEEES
                     SLLAYLNKGNNNNYSMVLGGLIVGIFGFFLSRKLIRKEDNSDLD"
     gene            complement(17614..18339)
                     /gene="aat"
                     /locus_tag="PLF66_01500"
     CDS             complement(17614..18339)
                     /gene="aat"
                     /locus_tag="PLF66_01500"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008590160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HMZ62736.1"
                     /translation="MEDFHKFFGDPLRKMDDLVAIGGDFSSDRLLYAYMHGIFPWSQN
                     PIRWYCLHPRAVFEIDGLHVSKTVRKKIRKNSFNITFNKAYSEVMKGCANRVNEITWI
                     TEGFLRGYTDLHKKGYAHSVEAWNQKGELVGGVYGVAIGKFFAGESMFSTESDAGKVA
                     LTYLFEALFRDGFTLFDTQQLNEITWNLGAYEISKEQYLDRLYKAVEIPDKWNPPSTF
                     NPEKFLIYDFEPRYGLWSKDLAE"
     gene            complement(18345..19511)
                     /locus_tag="PLF66_01505"
     CDS             complement(18345..19511)
                     /locus_tag="PLF66_01505"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016546252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HMZ62737.1"
                     /translation="MYSFRKFLISVNTFVLLLLANCATPSFKDFVSPDEKSIQFAAVG
                     DIMVHATQLTSAYNSQCKCYNFHPVFAEVKDTISKADIAFGNLETTLPGDSKLFAGYP
                     QFGAPDELAKALKDTGFDILTTSNNHSVDTGRKGIDHTIETLDQLGILHLGTYRSLDE
                     FEKNRLLTVKINDIKIILLSYSYSTNGIAIPKDKVVNLIEKDRIREDIELARKAKPDL
                     VLVAYHFGTEYARYPDKFQKEMVEFAFQEGADIVLGGHPHVLQQYEVDWIQDKYGEKK
                     QRMVIYSLGNFVSGQIKRYTNGGIIFNFRLTKKSIMGKPQILIHDINYIPVFVYVSNE
                     KNGFQYNVLPVEKYLKNDQPLKLTPTAYRSMLEFYEDTKTHLQKNSLVGVNDFR"
     gene            complement(19874..22771)
                     /locus_tag="PLF66_01510"
     CDS             complement(19874..22771)
                     /locus_tag="PLF66_01510"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HMZ62738.1"
                     /translation="MFFFKFLRKTVVIPLFFFGLLSIVGDGANSNIIYISNFLPYKSK
                     EEATNRDSILENLKNELTAKGFEVRIINGSNSDNLKVAKENNAKFYLSGFYGRKPNGN
                     LILYGHIYNPDKGNIIDALNLSDSLGDVSEVNLPPEEIKIEDNIVIDRFTKKISQKVR
                     FNTARKESPENVDEYVISTSIGKDQGLPVLAVETKSTEDVFKLLRDTEVVTASRTKES
                     LIDVPASTMVITEQDFKNRGYTSLDDIFKDLPGFDYIGQQGTDNAVLYQRGYRTPFTS
                     RTLLMINGVIENDLWAQVATPGRQYPISNIKRVEVIYGPASAVYGPNAFQGIINIITK
                     DGKENNGKAIAGKTSFMYGSGNTWVADGGVTSQIGDLSLALSARKQEGNDANKNQSNY
                     GYNTQYWINNPQIWGPLLFYGSGGKPFGKYSDPVQNWGTIISATYKTLKIGANITDKY
                     EAYGSQYPGDKAQPTAMWGKSGVNVYAENQMDITSKLSSYSLAVYRDSRIYGDWAEAD
                     GYGYGAKSYSSYISFTRWNEISKSILVNQNVEYKISDKVKILAGLKVEFKKLTKSYDI
                     PGYWWGSSYVSSIDFLNPNVNKGIDKLFPNGYAIGQSSDPFILKGPSPKNKMPEENTI
                     GTYDRGGFLLSIIDLGKFRFSPGIRYDENSIYGRALNPRITGIYKITDATAFKLLYGE
                     AFNEPSPLNLFGGYSGRTADLKLRPEKAKTTEAIFMTQGKQTLNEFSVYYSRYEDVIK
                     ESPQNAGRRRIYGFEYKFRWNVQNFLSNSAPLNFYLYYTYTRALADTYYDHNLKKWQE
                     GTTPLGDYEYLYSNEAKFIPRNREYTNLGDIAPHKINMGINFPIKELFIVNFRGNYVS
                     RREYYSRNPLSDSGPLSTLDTDNVSRRIVQQQDKTLDPYTVFDAGLTINFKDYGFFTL
                     KIMNVFNTSYFHPGTGQANAGNYYYARSLGYDSSILPQPGRTYMVNLTLTF"
     gene            complement(23539..24252)
                     /locus_tag="PLF66_01515"
     CDS             complement(23539..24252)
                     /locus_tag="PLF66_01515"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HMZ62739.1"
                     /translation="MKKNTFIVILLVSFFVTSCESIGKRTVIGAVTGCLAGLLAGAAY
                     DEAVKTKMSGGKIDDVVKSAFKKKKSSNKGKAVGLAAGCAIGLGVGLYFDLMKDDIKE
                     DLESKKIGVAEVKGSNGDTEALNLKLGEQAIKFDAGKAEVPASGKATLDKIAESVKAY
                     PDTKINISGHTDGSGDPAFNKALSQKRADSVKDYLKSQGVNDEQIGNSIGVGAEKPAP
                     GVGPNDPGQRRVELAVTAG"
     gene            24351..24815
                     /locus_tag="PLF66_01520"
     CDS             24351..24815
                     /locus_tag="PLF66_01520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62740.1"
                     /translation="MKISIRMSILCSLFGLVTGIFVSNNATGEGYEIFPIFSFVASLI
                     CSFLLWHYLIVKRKSNSILMGISVGLIIVILSHYFTWYFMALYFYICSSFSVKCLSSL
                     GEKTMNPIQSIYLLVPYTVLSLIIAWITLPLGGFIGAIGVKLQNNTKALEKN"
     gene            24876..25283
                     /locus_tag="PLF66_01525"
     CDS             24876..25283
                     /locus_tag="PLF66_01525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62741.1"
                     /translation="MKHFFIVLSLLFAFNLLATGEVEGTYTSKQENAKEIYELNLNIE
                     KDDTGEDAYSFHLTKAFQEESGKNETSIIGKYKIDKKNIILYGESITDNSNLENKQKY
                     KGSPIRVHVRSEDNRTILVLSAFGKILKLAKQS"
     gene            25286..25789
                     /locus_tag="PLF66_01530"
     CDS             25286..25789
                     /locus_tag="PLF66_01530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62742.1"
                     /translation="MRTRTKFKIILLLCLFYVGRVYAEDREILEIRKYYKSVESNLKN
                     LTQSKIDYIHKTSHVDETDLLSETIYFYKDKNQIVKIVGDKVFDCSGSVNELTYKNKD
                     LLFIYTYDWMGCNREAVAKESRYYFKNKKLIRWLEGKEKIDNSKFPAKEKELLKLSNF
                     YYLKSPN"
     gene            complement(25818..26522)
                     /locus_tag="PLF66_01535"
     CDS             complement(25818..26522)
                     /locus_tag="PLF66_01535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HMZ62743.1"
                     /translation="MIRFISILYIIFVVFTFPLFSKPSITYKPGDRLYVLSKSGLNLR
                     DKPSRNGRFLQAVPFGTMALVRQITDKELDVDGIKGFWVKVIADDREGYLFDGYLSLY
                     PPPVATLPSRNTITLKQYAEKFLPKDTKVTQSTDDSAVTTSTLILPLARIEEAYLLGV
                     CFLLNFDRYKFPIKPDKQKSADIEIDFDLSRDKNGKITNVSVTETYVQSGGTVSLIVT
                     DLGNKGVEIKTIFSEP"
     gene            26633..27715
                     /locus_tag="PLF66_01540"
     CDS             26633..27715
                     /locus_tag="PLF66_01540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62744.1"
                     /translation="MHFSKTKLILFVIFFSITSHLIFNSIILYTEKTSFIWNQIGNRK
                     IEPKTYDLAILGDSQLMSGVHPTLLKKYLQTESKDWEILYYPRPSEQPEGIYQLLHQF
                     EKSDVKIKNLIVNISPVTTSKNTIVDAHKSLTQNFQPFSTEIIKDLSLNKFYLKNLSG
                     NVYYLLLQVFPLLKLNGNFSNEVRIIPGSEGIQNDKHIESYLNVGFFGNLNLNKEKNN
                     FLSNHLIDQGFYFEWGNYTTYTGDCILRQESLTLPFGIEAAFLNPRNDSLDMWKKIGE
                     YSKEKGIHIYYLYIPFSPEAESKIGSKKNTSPIQISLNSIKAIFGNKSVLEIDSNLFS
                     NEDFRDYTHLNICGMMKLTKQLAKSF"
     gene            27727..28041
                     /locus_tag="PLF66_01545"
     CDS             27727..28041
                     /locus_tag="PLF66_01545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62745.1"
                     /translation="MYILRKLIFVLLSFFIYCTNVNDLYYSAGEANARILVAFAIKDA
                     ECKYTHRVTAFIPGEARKTEITACTQAILTKLCGEWNVTDPTPLLCKTINYRNNYKLR
                     GI"
     gene            28043..28504
                     /locus_tag="PLF66_01550"
     CDS             28043..28504
                     /locus_tag="PLF66_01550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62746.1"
                     /translation="MKFDLQQTEIMLYISLVIAVIILILFYFRSSRKRKKIFRPITTE
                     DNSPKPQKEQSILEIFDYLGTKIVHENGTYTVNHKGKVSFYKSWELVPKEFQKMVKEL
                     DNRSGLAKKNDDYFLEVINGIYYLTMPDGSIKKYNRLSDVPAYIRKAVGKL"
     gene            complement(28512..30305)
                     /locus_tag="PLF66_01555"
     CDS             complement(28512..30305)
                     /locus_tag="PLF66_01555"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HMZ62747.1"
                     /translation="MLRFIAIILLFIIPIFSEDSDSLILETPKVFFSPNYDDINDTMV
                     FQIKYSGSKTPVDWKIHIADENGKVIKVFSADLRRKRKGNFLRYLFSNDKELEPLKVT
                     IPEKIEWLGNDNVGKMLPDGRYIHQMKIFFSDKSEIKSKPVSIYLDSIAPYAKLSTEN
                     RFFTPNGDKIFDQLIIKQDVSADPSDRWNGVIYNEQNEPIRTFQWETRTIPKILNWDG
                     KDDRGILQDDGVYSYQLTGEDFSQNKMTTQLENLYLSKSSDNIDIQPNLSIFSPNGDK
                     LKETVIFKPIANEKFISKWEMNIYEKDGNKENTIRNFSGEVLPTSIEWDGKNTNGKLA
                     SDGTYFYRLKCVVGSKNLKSPERKVTVNTKDKKIYFKLSEKSFTPDGDETSDYLEIFP
                     ELENFTIKTWKLTILQKYPVGDTVKTQVIKKWKGIGQPAKRIVWDGYTDQGFLIGSLS
                     NLELHFSFRNELNELLFFKVKEFSTGILVTGKEDKLRISIPESTLKKEESKILNEIKK
                     LLSLYPGYRVQIQTHSKQPGEDKVNMKRTEARARDLFKKMFDRDMDFERYTYQAYGEV
                     EPLFYDEDEYKQELNDRFDVLLTFPEQPKKK"
     gene            complement(30309..31868)
                     /locus_tag="PLF66_01560"
     CDS             complement(30309..31868)
                     /locus_tag="PLF66_01560"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HMZ62748.1"
                     /translation="MKKTVKKNKTTFSSKNNSSNSKLNKSKNASFEILNPATLTKIGE
                     LPLYSAEMVEEKILLAKQAQIIWANKTISERAKEINQFKKFLANNKEEMVSCICNETG
                     KTKMDALLEVFTTLEAIDYIARKGSKFLSSEKRSGGLFINKNCYVNYHPYGVVGVISP
                     WNYPLILSLTPIMTALMAGNTVVIKPSEITPYTTLKALEFFQRAGLTNGILQVVTGMK
                     ETGEALVNSENTNMICFTGSTQTGKSIAEACGRMLKPVVLELGGKDPMILFKDADLRR
                     AVRGALWGGFANAGQTCISVERIYVEKAIYNEFISLLKEEYGKIKQGSKEEFPSVGSM
                     TVAKQVEVLESHYRDAKAKGAFFVSGGKRLLSEKGLYHEPAIIIGVNHDMKIMTEETF
                     GPEISIMEFNSEEEVLALANASEFGLSASIWTSDIRKAKTLAHKLQVGSVCINDVVAN
                     YMISDLPFGGFKKSGIGRVHGKEGLRAFSQVQAVSYNRWFWRFSDELWWFPYSKKIYG
                     YVSRAIKFLFG"
     gene            complement(31843..33870)
                     /locus_tag="PLF66_01565"
     CDS             complement(31843..33870)
                     /locus_tag="PLF66_01565"
                     /inference="COORDINATES: protein motif:HMM:NF020855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HMZ62749.1"
                     /translation="MKKNILFYILLCLCGLISLAAQDKEAGEYIQLGEKFLKEKNYRE
                     ALVSYKQALIKNPGSIRANLGFAKSSLNLGSKQDAETGFKRTLELDSKNREAIAGLAE
                     IQADLGKYKEAMDLLESSLKEEPYNPELLLARAYVLLKAGKNKLALIKLEEAKKRVVQ
                     NYEFKLLLAKVYIANKDFKKANQIVDDLIAKHPENPNSFNQKAILNFEMLKDSSDTYR
                     LMKETYTLLHTALSLDGNNHESKRLLIKNLLWLGKFDTTDNLEKYAEAKKITLDLLHD
                     YPNDPYLQYISGYISYKLNHGEDSAENYLRLLELQELNELGRFSAENYAISKLNENHT
                     LRVTLGKYRLDRYRATRNEYLYNEAFFHLKRAEKLIPTNSELKQELLEYYYRNGDLYR
                     LLHYLTKMRDDNVDDMKIHNRLENVYHKYKQTLIYREGFTSESGRMNQGFRTEPEIFI
                     FDPEPDKSLYTYPDASLQIANAIKFAISLHPNLKLISGSEEAQIRNAIKKKKGVEAYT
                     ESVYYSPDSIDILDIDRKEGSRIRYIAYGTFTEDKNNLTVRYKIYDRVSGKIIDTINT
                     TASNRNSIAEISVRVAKRISNSIPLDGRIIKINQDGVIVNLGTKDGIDKDFILEAVIP
                     GQDNIKMKVITVDEYVSKAIPEEKGWGKNLSLRDRIIVVGKKNNEKDSEKK"
     gene            complement(33867..35948)
                     /locus_tag="PLF66_01570"
     CDS             complement(33867..35948)
                     /locus_tag="PLF66_01570"
                     /inference="COORDINATES: protein motif:HMM:NF028479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HMZ62750.1"
                     /translation="MFKLCHKVGIITRQFLKFSPLLILLVLQPLAPTEELPEFRLGKE
                     QARTYFKQGLVYLHNYQYNAARESFIAALSIMGDFKLARKYLSDANYLSGEWQESLNE
                     LEIIEASGKMNQIWKNRAEILRLHIAGVGKNDNLTFYKHLSGDENRGYRFRNPTDILF
                     DEEGNLFILAFDTANIIKLDTNGIPVGNYKGSFGRTFEGPLFFTYHKGLIYVSDFASD
                     RIYVLNDKGYFQERFAGKGSEGGKFYGPTGISVSEKDILYIADSGNNRVQKLSLDGKY
                     ISEFGKDGKGKLKMPSGLTIENNLVYVADKGNKRIAVFDDEGNYIKEYTHPNMTVPRS
                     VKFFKKRMYVADEQNGLMIYNMDSDKWTKIASFRDETGKYVKLLRSFASSYDTTGSLY
                     SVDYDRHRVDVFAPKNTLTSNLNVFIERIELNRFPDVSLFVRVRNRSKQDLTGIGRAA
                     FRVTENENVYPLVGLAKMKPFNENMSVALVYENSKKISEFSKNIDGVLGSFFNSITVK
                     DKIEVIRAGKDAEKIYSFGYSPLDIYAKIRKSEPTDSSINLGKSLYQGIGDLVPELGP
                     RAVILLVSGDVLPSAFNQYTVLRNIQYANAHAIPVIIMSLSDEGEMASVYKDIANRTG
                     GMYMRVPGSQEETNLYKFIQSKQDKRYIISYKSKLDPDLAGRYIDVEISSFYRDIVGR
                     AQSGFFVPEKQ"
     gene            complement(36004..36888)
                     /locus_tag="PLF66_01575"
     CDS             complement(36004..36888)
                     /locus_tag="PLF66_01575"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HMZ62751.1"
                     /translation="MNDRKTVGNNPFATIKSIKPPVKEGAPSGASDSELSNVVYSLSE
                     TIHEFSEMADDLNKNSSKLEKQTSQASNASKQLSHNVIAMGSSLEKLKTGIKKISSNS
                     SNSTKSSITALKTADTTNNVFSNLLESSKELDKIDKIVAGLAQQINLFALNASIEAAR
                     AGEAGRGFAVVANEIKELAKETNKIADDIRYKIENFYQDSKRAVESMNEISSQLLSIK
                     NNQNDIDSSLEEQVNSLNEIAKNSSNVVKSSNSVMDNILNVYKLAKMASEGTEKSLRY
                     TNTLANLTNDLKTLLDNK"
     gene            complement(36898..37668)
                     /locus_tag="PLF66_01580"
     CDS             complement(36898..37668)
                     /locus_tag="PLF66_01580"
                     /inference="COORDINATES: protein motif:HMM:NF018290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HMZ62752.1"
                     /translation="MRKIVINNQKGGSGKTTTAVNLSAALAEKGKRVLLIDLDPQASA
                     SIWLGLSSSVQDKDLFDLHAGPEVILALAQKTKIRNLEMIPFVPLKNKYSKEMKELPN
                     KASILKNKFAGIDPKRWDYVIFDCSPGLNLTTLNAMGAANEIIIPVVAQSLALYGVLS
                     LLETLESVQAKLNPSLAITGILPCRVDQNIKHNMEIVDILIEKFGVLVYNTYIREDIK
                     IAESPSFTQTIFQYDPKSIGAHDYRSFAAEVIAQENKK"
     gene            complement(37687..38127)
                     /locus_tag="PLF66_01585"
     CDS             complement(37687..38127)
                     /locus_tag="PLF66_01585"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HMZ62753.1"
                     /translation="MTVKKFCTFLIDGNFFGVEIGKVFALVKDIPMIRVPMASRFVKG
                     LIQYRGQIITVISIRKVFDLPEIELGENYAHVIVRTKYGLVSFLVDELKDIEEITRNI
                     RIEVPKVIHGVDRYFVSQAYRIDETLLLILDSEKVVEFRSDRIS"
     gene            complement(38124..39800)
                     /locus_tag="PLF66_01590"
     CDS             complement(38124..39800)
                     /locus_tag="PLF66_01590"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HMZ62754.1"
                     /translation="MEKDQIKLFLDESYETLNSLDKQLIQLGKNLDQREHLETIHANY
                     KTFLDSAKLFGFNKLASMVLIGEKLVDRLKSSEYDLTNEIITIILKLNYSIRDIIFSI
                     DETGKEPQIINSSMVSDIEELINKQESGEEFIIASSDDEDEDSSRVSSTDIPIALTPI
                     FTENETMDSLMNTIIPLLQTQVQIKELASNDKNSPLETHARKLEFLVRDLYTKVHGAK
                     TKTLGTLLHNFDKVVSDIAQMQDKQVSFLIEGGEKELDTRMLDVLRNCLIHIIKNSVE
                     HGIEMPEIRTELGKSAMGEIHLNAFHHGELFYIKIKDDGSGIEPARVKRKLIEKNILL
                     TEEAERLNDEEAIDFIFQQGFSSSKSSAGLDIVRSNIEQIAGKFYIYRNIPGKGVEFH
                     ITLPLIDEIVPSVIVSVGKERYIVTRANLYEIMQTESDSFINTLEVINGFPTYKHRGD
                     LIPILFLSQILKYEDDKEVADRQRDAGKKISILIVEVNQFKYGLVADSVEDMNEVVVR
                     PLNFEIKNVYLFSGLTVLKDETPALILDVGEIMRVHLKHLDLKVVEEVME"
     gene            complement(40659..41057)
                     /locus_tag="PLF66_01595"
     CDS             complement(40659..41057)
                     /locus_tag="PLF66_01595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62755.1"
                     /translation="MRNKRMKLTVSIFLILFFHFISCATLSNDENIESSNHWSDYQGE
                     LNWEDAKRKCGSLGLAGYGMRLPSIDELEIAYKKGITKSWKSDWQQTKNSGYWTANHY
                     SEARAYYFLVELGELDYLNKKDIKHVRCKR"
     gene            complement(41061..41939)
                     /locus_tag="PLF66_01600"
     CDS             complement(41061..41939)
                     /locus_tag="PLF66_01600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62756.1"
                     /translation="MKFPRKKSFILLLFLFCFTNLNSFGGSSSQYLEWYRRQVQTEHR
                     VKPCNQKSNTIVERLKHKHSFLYLKIPELSNEYLEYGNEESDQNLESYRRTEKSLPIL
                     IEFGDNILNPRKDDFMNYQVSSKIKERIISENPTIKRRNCTFMALGSEYLFPVPSGAG
                     ILSIILYFNYAQDIARYELLRLLRGSSQMIPENEFDNFIHRSLRTEVNFKPGQIYQIE
                     IKTNPNSAISRKSYTAWANAFDRMNTVPGFTITLKELPPNTKFAFDGKDDVGKDYMEV
                     KKEFIFFETGLEKNIK"
     gene            complement(42042..42575)
                     /locus_tag="PLF66_01605"
     CDS             complement(42042..42575)
                     /locus_tag="PLF66_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008593948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HMZ62757.1"
                     /translation="MKYLFESERHGFRKWRESDVSRFTNMNSDLEVMRYFPQLLSREE
                     SINLINRIQSHFEKWNFGLWAVEEKDSENFIGFIGLYYADFQSSFTPCVEIGWRLDSR
                     FWGRGYATEGAKLCLQKGFEEFNLNEIYSFTSILNTKSENVMIKIGMQKVMDFEHPNL
                     EKDHPLCKHVLYRILYD"
     gene            complement(42831..43130)
                     /locus_tag="PLF66_01610"
     CDS             complement(42831..43130)
                     /locus_tag="PLF66_01610"
                     /inference="COORDINATES: protein motif:HMM:TIGR02607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HMZ62758.1"
                     /translation="MLPKNRKSTHPGIILWKEYLEPSNITQTQFARHVGLSLQTINEI
                     IKGKRNITPETAWLFAKSFGTTPELWMNLQMNYDLTVRKPVDKLKALHLLKLSAA"
     gene            complement(43148..43438)
                     /locus_tag="PLF66_01615"
     CDS             complement(43148..43438)
                     /locus_tag="PLF66_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010410382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HMZ62759.1"
                     /translation="MIANFGDKFTEDLFHGNTSSKTHKFPKELVEIALRKLDMLNAAL
                     NVEDLRFPPGNRLKKLKGDLKNVYSIRINDQYRITFQVLSNKFENVKIEDYH"
     gene            44141..45511
                     /locus_tag="PLF66_01620"
     CDS             44141..45511
                     /locus_tag="PLF66_01620"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HMZ62760.1"
                     /translation="MLAFITLVPLITLYGILYLTGSNLISISFFGNRLDLVYFDQLSQ
                     IMLIYISILSFFIISYAKKNFERERNGSKWLFWFFISLLSVQFLVMNGDLLLTVLAFI
                     SISLSFHNLLLAFPQRQKSKLAATKKFILSRLGDLSFLLGAFFFYKSFGTFNIQDVIS
                     HLNDLDLQTRQDLIFLPSIFIAFACLIKSAQFPFHFWLPETIDSPTPVSALMHAGIIN
                     AGGFILVRLRFLYEPFNLASILILGLGLITIFLGGISMLAQSDVKRKLAFSTIGQMGF
                     MMVEFGLGLYPMVILHLMGHGFYKAYGFLASGSRDIVPSPKLHSKLYRTLLAGFVIIV
                     PQVVFIATNHLELTLVSIFIITLIGFLPLKLIKRGILITITLVLFVILIPEYAMKITA
                     IPYSTNQVPYMISLSVILCLSILSGFTLSLPFLSENSFVQKLYCYAQRGFLSNYLSDK
                     FIYKLF"
     gene            45522..47705
                     /locus_tag="PLF66_01625"
     CDS             45522..47705
                     /locus_tag="PLF66_01625"
                     /inference="COORDINATES: protein motif:HMM:NF021578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na-translocating system protein MpsB"
                     /protein_id="HMZ62761.1"
                     /translation="MNALSTNPNNQKLNTDSIYQLLKKVPPLWDLDSYIAVNPFLGYS
                     NQPFVKTMEKLESILSMNLIPEKDLLDGKEIKHENVGEFDMNYANLSQLIGPFLASYI
                     DDSISYWKNPWKEKSLWQAFQSWSAIDPVWNLTFANFPVLKDLPDAAIQAIQKLGEEN
                     SSIQVESLEKLLFILPGWASYFRKNNWFKELEPNSDLVGLLAILAYIYTYRPELFTVN
                     FKLKHLDNSEKFQNRFDYLKKKEIEYRTKLLQDLKKNYPIPEEKKSDTLPKTKFLFCI
                     DVRSESMRRHLESTSPSIETEGFAGFFGMPIGWKHWTEEEFSHSPALISPAYVFCSNE
                     KSFSLRKNKLQTKSWIQKIKRTFPIGFQYVETAGFLSFFGLIYKTLKIGPLHENPREI
                     SSEEIKFALSSLPQTDKTNIAAGILNHLGLKDNFPSYFIITGHGSLTVNNPHQAGLAC
                     GACSGQSGELSAIFASQLLNDLETRKQLMQMGISLPAETKFIPAMHETVTDEIIFLEK
                     ENIDKSYLQKISELIKKASLENRKEKARIMGLNSESPYVRSKDWAEVFPEAGLAGCAS
                     FIIANRSRSKNANLEGRSFLNSYNWKKDSEFKTLELLMTAPAVVASWINLQYYASTTN
                     PDVYGAGNKLLHNVTGNKGVFEGNHWNLKSGLPLQSVFDGKRFVHIPIRLQIIIEAPQ
                     EEIDKILNKHSSIGDLVKNEWVYLIQWDENGDFYLKNQTWEKIKI"
     gene            complement(47887..50040)
                     /locus_tag="PLF66_01630"
     CDS             complement(47887..50040)
                     /locus_tag="PLF66_01630"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HMZ62762.1"
                     /translation="MSDTSSKMKRILIVDKEIIFANALSKKIQNWNYNVSIATSEIEL
                     VEAFQVEKPDLILFAVDFGLTISREISLRDNIPIVFMFSHIEPSKLAELKSISRYGFH
                     SKNSNDYCLQNSIELALELYDSEKRLSEQISIEPLSILLNNDINSLLDLISDSIVAFN
                     LDWRYTYVNKKTLNDFGLKEEDVIGKVYWELFPEVMGTPFLEEPKEAMRTQKKREFSA
                     IYESYQMTIFIECHPFPNGILLIYRNISEKKKFDKKIKDTTNFLNSIIENIPNMIFMK
                     DAKDLRFILFNRAGEELVGFNRKELIGKNDYDFFPKEQADFFTKKDLDVIHNGQLVDI
                     PEEFINTPNGTRILHTKKLPLFNDRGEPEYLLGISEDITDKKEAEKTIQENIERYHRL
                     TETVPGALYDYVLHPDGTSQMLYVSPKCLEIFELDPVDVMRDMTILWNLVHPDDIEQF
                     KKDSDEAFHSKDHFSTEVRFIMPSGSEKWVQVTSRPNPKKDPDQPVVWSGFAIDITFR
                     KTAEEKIKTLLGEKDLLLREVHHRIKNNMLTITSLLYLQTDSLQDKVAIEVLNDAISR
                     IQSMVLLYDKLYMSSNYQDLSLFEYLIPLIDEIVYNFPNYRIVKVNKNIENLILPSST
                     LFSVGIILNELITNAMKYAFIGRESGLLTVSAYQEKDQIIFKVEDDGVGISSEQESKK
                     GFGLQLVQMLSKQIHGKFRIERSSFSQFILSIPNR"
     gene            complement(50125..51447)
                     /locus_tag="PLF66_01635"
     CDS             complement(50125..51447)
                     /locus_tag="PLF66_01635"
                     /inference="COORDINATES: protein motif:HMM:NF013661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HMZ62763.1"
                     /translation="MKVRISIFILSILLSCVCDTRITRSNSYQVDLEIIPYQDWLSSE
                     LHSKIIGVKRNTYISSLILHHTDSKSKEQYIKDSLDSGFLVHFIVGKDGKYYGWNTIP
                     YLAVKAVPRMDDVSLHISLEGKEDEILNNKTQLNAAGKLIKKLINDLEIPFSNQNINS
                     LSGVFTHTQAKKKFGNFVDLNECGSEKILKEIFSSFGGQYYAEENWFGRYERDWVSRR
                     EKKNKEIPEPDFDRGRGITKPTQVELVELEKNEEGFTPENYRLKYVFKQKIKPECVVL
                     HFTAISSFVVSQETLERRKLAATIMVDKDAKAYQILDYLDDMAQAATGTNQNCIQIEI
                     VGKNMDELMANNRQADKVSRLVKELTDKYNIPLTNYKIEDLKGIYSHTQAKKKYGGSI
                     ALYGKDFDPGEPYMKRIIESIGGKYYPEKEWHNRQSDDWIILDQEFQP"
     gene            complement(51471..52154)
                     /locus_tag="PLF66_01640"
     CDS             complement(51471..52154)
                     /locus_tag="PLF66_01640"
                     /inference="COORDINATES: protein motif:HMM:NF024587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HMZ62764.1"
                     /translation="MARNSILDYIKEKINSDSVNSELSVPRVITISREYGCPGIPIAN
                     EVSKALTATNKAEWSVVDKQIVNQAAEELDIPAKLIDQIAKSKPKGVFEELFLAFSDI
                     HLPSDLKIKKTIARILRTVALHGNVVILGRGGAVLARDLQKAVHVHLHASVSWRIGRV
                     KNLENLKSDAEAIGRINVVDNERIFLRNYFAGESPGLNVFDVSYNCEYLSQEEIVKSI
                     LQLAQMKGI"
     gene            52298..52609
                     /locus_tag="PLF66_01645"
     CDS             52298..52609
                     /locus_tag="PLF66_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016550941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HMZ62765.1"
                     /translation="MVSKEQSLKELRTIPGVGKVVANDLWNLGIKSISDLINKKPENL
                     YHKHNLLKGKVQDRCMLYTFRCAVYFANTEKDFQEPEKLKWWNWMDDDKKTHHKKAGN
                     Q"
     gene            52606..53055
                     /locus_tag="PLF66_01650"
     CDS             52606..53055
                     /locus_tag="PLF66_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012390071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HMZ62766.1"
                     /translation="MKELTPDSYINNLSKERKEVITKLRDLLKKNLPKGFEETISYGM
                     ISYVVPHSLHPEGYHTDPKQPLPFISLASQKNFIALYHMGIYADEKILHWFVTEYPKH
                     SKTKLDMGKSCIRFKKIDQIPFDLIQELVKKMTPKQWIDLYKKSIKK"
     gene            53267..55717
                     /locus_tag="PLF66_01655"
     CDS             53267..55717
                     /locus_tag="PLF66_01655"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HMZ62767.1"
                     /translation="MLKTILKRIFKDKARFYFFTIVFFWTGSVTLSLLWNIFSVKTSM
                     ESIAYVTAKSSFEKDINYRYWNAKHGGVYVRINSDTHPNPYLKIPERDLTTIDGIKLT
                     LMNPSFMVREVFDISKQTSEIQSHITILKPINPNNIADEWEQKALREFETGVKEIKGL
                     SEDKSHFRLMKPLYVETSCMKCHRDQGYQVGNVRGGISISVPMQPYKAEANLQYYSLS
                     IGHFLLWILGFIGSKIGFNRYTQYQFSRKDIELQKKELDSLQLIMNGVAEPIVVIGMD
                     YKIKISNPAFRDFFGATEEMTRQLYCYNVLYNRSEACNSTPDNPCPLLHKYDKNGKLW
                     SIRKHTRNDSEVRTTELLATPYYNTEGNLKGTIQVFRDVTERENAEAKLKHLAHFDTL
                     TQLPNRLLFEELLEDRLLTASIKNEQVALFFVDLDRFKNINDTLGHPVGDKLLVKIAN
                     RLRQMIISPNIVARFGGDEFTIFLSTKNNPQAIKDMAELIKKEIAIPIILDTYEFFVS
                     ASIGVAIFPEDGRTLIKLMKSADTALYRSKSKGRNLIQFYDQSSETNSRKRLQIESEL
                     YSAIEGSDLLTVYQPQVSARTGKIIGFEALCRWQHAELGLISPNDFIDVAEETGLIVP
                     LGKKVLLTAAKQCKIWQMNYGLPLRIAVNISPRQFVRQDLIKDLLEALEISGLNPKDL
                     ELEITETSIIHDLDTTIHTLEKILELGVMVAIDDFGTGYSNLQSIKSLPIHSLKIDKS
                     FVNGVPEKDSDRAVIKAILALAKNLSLKVVAEGVESKEQSEFLASEGCDILQGFYFGK
                     PETAKVVETKFLSSNHLT"
     gene            complement(55718..57169)
                     /locus_tag="PLF66_01660"
     CDS             complement(55718..57169)
                     /locus_tag="PLF66_01660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62768.1"
                     /translation="MIEKANRRIWGLLVLAAYTIYISYIAYHHELWRDEVDVWLFVRD
                     TDFNGFFRYLRNSGHPGLWHLFVLPFAKLQFPNFTFQILHLFLAFSSAYLLLFYSPFP
                     IWIQFSILFSYFFIFEYSVIARNYAIGILFLFSICAFFEKRFEKPFIYAFLILLLANT
                     NLYCSILASGFALMFIWEVLEKRDFTIRNISSIAIMGLGGILLILQVFPSSETQGSAM
                     REFKVFNNLGSVISILYSFTPGISKLDFRILTGFILLITLGILVFTNKKLFFFYLYSL
                     SLLWSFYTFIFINGYRHGGFVFLTFLVTLWIYASEKKETSFSSTNKYFNLRNILLFFS
                     VISLSQSIPFGWKETRKDIRYSFSNAKEMASFIKQNDLDKVENIFSVENADKAKTILF
                     YLQNQKQFYFPVIKSFGSHMLWNKVMVDGEIRPSYSHLDHTIEKFRNQNNIYFISSNP
                     IQDEHLNLLYQTKDRNEFIADENFFLYKINYSK"
     gene            complement(57171..58652)
                     /locus_tag="PLF66_01665"
     CDS             complement(57171..58652)
                     /locus_tag="PLF66_01665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62769.1"
                     /translation="MIYNLIVKKYFLLSLYILITTMIAFHHELWRDEVDVWLMARDVS
                     SPKDFFAYLSNSGHPGLWHLILLPFAKLNFPAFTMQIVHLSIAIASVYLFLSYSPFPD
                     YIKLLFVFSYFPLFEYSIISRNYGVGVLFLFATASFYTKRFEKPILYGSLVLFLCNTN
                     LYCSILASGISFIYFLEIIQERKFEKNILIGFMIMVLGGLLLILQVLPNPETGEHKSI
                     FANMKNFLAIPVSLLYSFSTGVSKFNFLILPSVFILPVAFRVFYKTKKPFFFLIWVMA
                     ILFLFYTFFYMRSYRHAGFVLFAFLFAYWIYYTYSERIISNSLILDIRKKEFYQSENI
                     LQVLFIFSLLISIQFSFQTAKKDFLYSFSNAKEMAEFIKKEKLDDIENKIATHSADSG
                     KTVLFYLDSIHSFYFPASDRFGSFNPWDKKAWKGEEMEGEKAYAITMEKFKNVENVYY
                     LASSALPEFTKGATLLYKTKNRNEFIKEEEFYLYKLDTATKEK"
     gene            complement(58681..59826)
                     /locus_tag="PLF66_01670"
     CDS             complement(58681..59826)
                     /locus_tag="PLF66_01670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HMZ62770.1"
                     /translation="MKDDKMKYKVFSIILILFLFGFISIILEIGPRLFFQGSGLTEIP
                     TPSKIDPYKANPYIINFRPNIYSHIPLAKYTQSRADYAVDYTINSFGFRAPEYNLKSD
                     PSKKRIVFVGDSLVEGHGSEIPDTIPAKLEQELGTKFEVLSCGMQGASPIYYAFNLER
                     YLQFKPDYLIIGLYDNDFYEDRFREANYDNAPYLENPNFLVKNSYYPWLFKSRFVQML
                     YSLYYSKKKQKSELFQIMKKNQKEFTMFSEDKEQRELILSGSYKFTAKYREKVWNMSV
                     PYLEYIIRETKARNIPVYFVSLSFQSSDPNFKKDMAELAAKYLEFETKWASERSLPFF
                     AVNEYAKEYFRKNPNGKLHIVDDGHPTKEAYASFAKEIAEWIRPQLK"
     gene            complement(60055..60480)
                     /locus_tag="PLF66_01675"
     CDS             complement(60055..60480)
                     /locus_tag="PLF66_01675"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HMZ62771.1"
                     /translation="MSDFGLTHIALDVLDIDKSISFYQKYAGMQIVHRRVDANINVDV
                     VWLSDLTRPFVIVLLKSKKVEGRLLPQCHLGVACKTREDVNRLCELAKADGILLDGPN
                     EWAPPVGYWAYIKDPDGNTLELSFGQVVHFTIEQAANSN"
     gene            complement(60489..61199)
                     /locus_tag="PLF66_01680"
     CDS             complement(60489..61199)
                     /locus_tag="PLF66_01680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62772.1"
                     /translation="MKYLISKILLISIFLIIDCGSFYQTKHLVYSTRSSAFYRIPKDE
                     IKGKEFIQGKFIHPFPIQPEKLTDILGNLRFKKHTQIGSLNDYVFHLNELSTLTADLS
                     QVLENLKPEDCVMGISMFDHIESVISNNKRTTFLLWVDAKGLNLVLGEIQSDVARDTA
                     KNFLEWTQVPQISLNIVPDANEIDSEKGQLYTFNKVNGYTNKKWLIFPLTDLNIYQLK
                     DRKIPSVAPNASSGKKSE"
     gene            complement(61213..61560)
                     /locus_tag="PLF66_01685"
     CDS             complement(61213..61560)
                     /locus_tag="PLF66_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016547069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HMZ62773.1"
                     /translation="MILSSIRTNDHLIIKILTDVMMDNSKDFYKEFEGIIQRNSEVKV
                     VSFDFHKVNFMDSSGIGSLIRAASYIKNLHSTVTVFNLNKSLYSVFRLSGLDNIIAIY
                     TYEEFIEIYPDCK"
     gene            complement(61642..61833)
                     /locus_tag="PLF66_01690"
     CDS             complement(61642..61833)
                     /locus_tag="PLF66_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012388040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62774.1"
                     /translation="MKVGDQAKLLKKTFMQNGVFILTNSIVEIVDEESGLYHVIYNDK
                     EGHPHTIKNVKPEELQKID"
     gene            complement(61830..62618)
                     /locus_tag="PLF66_01695"
     CDS             complement(61830..62618)
                     /locus_tag="PLF66_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010569126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HMZ62775.1"
                     /translation="MNFEKFKEINDTRMNYREMEDATVVSNYRNVGCGDGYRIYLKID
                     ENERITDASYTTTGCGFGIAALAMATEVAKNKTVSEAESLSESDIEKLFEFPERRKNY
                     PESAIAALRQALKDYRTGAGIPKEKRITKSKALELLKENGNLSSVNLSSIILEKEKLD
                     GVDFSKSDLHNAYLQNGSFIGANFAGANLRGAFLNNCNLQNADFTGADLRWAKLAGAN
                     IEGAIFTDAIYDIGTRIDSNQIHIFKVMQKAGKDIYMKAEEVSQ"
     gene            complement(62728..62973)
                     /locus_tag="PLF66_01700"
     CDS             complement(62728..62973)
                     /locus_tag="PLF66_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019770973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HMZ62776.1"
                     /translation="MKEGIHPDYRDVIFKDISCDYAFKSKSTAKSNEKMVWEDGKEYP
                     VIKLEISSASHPFFTERAKTVITAGRVDKFKKKYKMK"
     gene            complement(62989..64389)
                     /gene="rho"
                     /locus_tag="PLF66_01705"
     CDS             complement(62989..64389)
                     /gene="rho"
                     /locus_tag="PLF66_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015681801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HMZ62777.1"
                     /translation="MASKKEEQSIEVEEDFEAQQQTTAPTNENGNGSHSRPFKKRKKF
                     EGPTPEPIDLVKLKQKAIGELTELAKSMGVENTNGLKKQNLIFAILQSQSEKDGQVHA
                     AGVMEKLPDGYGFLRSPDYNYVPGPDDIYVSPSQIKLFGLRTGDTIEGQIRPPKESER
                     FFAMLRVESVNGVPPEVANKRALFDNLTPLYPNQRLNMEFDPSHMDTRILDLMIPIGK
                     GQRGLIVAPPRTGKTILMQSIANAITRNHPEVYLIVLLIDERPEEVTDMARNVRGEVV
                     SSTFDEPAQRHVQVAEMVIEKAKRLVEHGRDVVVLLDSITRLARAYNQVIPTSGKILS
                     GGVDSNALHKPKRFFGAARNIEEGGSLTILATALIDTGSKMDEVIFEEFKGTGNMEVH
                     LDRKLSDKRIFPAIDINKSGTRKEELLLRSDILQKVFVLRKVLSPMSITESMELLLDK
                     MRTTKTNEAFLASMNS"
     gene            complement(64471..65655)
                     /locus_tag="PLF66_01710"
     CDS             complement(64471..65655)
                     /locus_tag="PLF66_01710"
                     /inference="COORDINATES: protein motif:HMM:NF014950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HMZ62778.1"
                     /translation="MKIESIYKHFMVTPVSHLPVVDENSDIIGLISKEKVLMEMSDVS
                     TTNEDYEKIPEQFLDFHITEGVIYYFQNNRTIPVLNLFSQKVDSWEKPRFLAEVSKLN
                     HESPSIKTDSNESASEEMTESKAAIFKFMSNVLASFPDALFATDKEGVTTFYNERFEV
                     DILARSIFRDSISYTEKYFRELNQDLFANYLKSHELDVESRKSTVPVLQAYVKDLSLV
                     IRIITLKNDQNISGFLYHFIDPQNRINRLNEEGYTFPSVEEAFHMNLPLETVIEEVES
                     HYIFQKLKKNDDNISHTANELGIPRSTLQNKMKLLKINEKFGREISTPIPRKRKKVVE
                     KKDDSSRKVLASESKRTTEAKSKKVAKVSSKKPSQTSKKSSQAKLNKNKVKVKTKSKK
                     KK"
     gene            complement(65702..66382)
                     /locus_tag="PLF66_01715"
     CDS             complement(65702..66382)
                     /locus_tag="PLF66_01715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62779.1"
                     /translation="MKYFLLICLMISFSLSANPKADLGERKKAFDSKAKTLLSMQEII
                     ISEKLIKDFQGLKDMMNDVDTTYRSGDKDKIKKTLTLAESKLANVQKDYAVFISAETK
                     ELMESYSSQVTAAEEKEGGKKTKPTISQTATKEKTSEYYSIAKADYANATKFERDGNL
                     QYAIQLFKRALNYTVLAFDHAKYSLPAKYANLSKSFSTSPETEAKEVPGGTHTPAKES
                     KPVTPAKK"
     gene            complement(66387..67490)
                     /locus_tag="PLF66_01720"
     CDS             complement(66387..67490)
                     /locus_tag="PLF66_01720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62780.1"
                     /translation="MKLKTLILISLFSAFLSLNAVAPDMENLNVLMNENKMHFEFINV
                     VLSNLIKPQAEPEKLSDYANNKIKPEAAAKRVIDKDDSFYYTKFLDANQRDFEGSLLY
                     FRGDYGDAHKPLKDAQGKLKELYEDALERHTEHTRVLVSYAAQRIIKSNDYSSKGLLK
                     QAFRELKIAEDYYQLGWNQMPYQFRNKLSLYEDGFRASRKARRFTLLAMMNYKTANDD
                     KPIYKKQSLSELHNSVNEGKVNDYEYMKTTLRNFIENKLIEGKISTAVTFPRPKGATD
                     YEFKANTTGSLDLMEILDDCYAIITYNRISILDETNNVIRKDSPGPVTLDSNAKPSPT
                     TGAGSETTQPAKATNTNPPVSPTPPTNPMEKKE"
     gene            complement(67641..68987)
                     /locus_tag="PLF66_01725"
     CDS             complement(67641..68987)
                     /locus_tag="PLF66_01725"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HMZ62781.1"
                     /translation="MKKWVCSIKKILVLLLVIFICITSPHPQEERDIKESSDEGKQDA
                     KETIEVEENKNQEEQIASSDILSIANQFYSDFIKKKKTPLHNENMKRMHRFAISYDSI
                     SKAHFNKGVTKLSSQRDIAELIDYALKSDWSEQVIKMTSDPIYGYSENRALRILITNH
                     TASHYYQIPYPTLFCLLFQESQFDFKVRSKTGAAGLGQLTSVAIRQLQINRKSKSTED
                     RLQSATAHIGRVYKDPKLNEILKEMGFTPDFPPLKSFPKNIVHAKRINETFAREIANQ
                     LQKQGHSFAKDLNLIRKLTDHVSRGNLAPRQYAAIHPVYLQTLAHAKNTLGNVLNIET
                     NILLSAMLLRYYMDYSWKVNGKRVHLTPEVRGIVAVAAYNQGPGGVTRYLNQFKQSFP
                     NKDLNKMTLRDFKDTFTSSRVANALRQTPAQTKEVFEHVWKVKLCAHEGIIRPRSR"
     gene            69171..69398
                     /locus_tag="PLF66_01730"
     CDS             69171..69398
                     /locus_tag="PLF66_01730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62782.1"
                     /translation="MKNFKPTYEELERKILELEKQILTFNKKSSMDDLSTNIESGLKR
                     FEKVKELTEPTYYNALITKNYKSLIVSHLCL"
     gene            69385..71571
                     /locus_tag="PLF66_01735"
     CDS             69385..71571
                     /locus_tag="PLF66_01735"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HMZ62783.1"
                     /translation="MYAFDKDGKCILLNKNLAELFQTNLNEAIGKKREELILDNNTKI
                     HRNNDLQIFESGISMQFQEEISFPDGIHTFLTEKFPLYDEVGEIYAVAGISSDITKQK
                     KVEKEIFEKHQLLEAIFQSAPMAVLCLDREGFVTMWTPSAEKMFGWREKDILGKKNPI
                     IPTEKLEEFEVAHSYIIEGNSLLGREIKRQKSDGSLLDIFISVSPLKDVNGYILGAIA
                     ICNDISNKKILENALKESEKKLRSVFDVLDVGITITDKEGNIIDCNKASEKILGITKE
                     EHLTRNYAGKEWTIVRPDLTPMPTEEFASVRALRENRQVANVEMGVLKPNNDISWILV
                     NATPMENTNLGVAISYIDITDLRKTEKELSKNLIEQNIILDNSNIGIAKTIDRKIIWA
                     NKQMCEMFQYTPSEILNLPTKSLYESEMDFEKLGKIGYDILKTGNTFTTEERMIRKDK
                     SSIIIRLIGKAIHPNDLSKGSIWILYDITEEIKAKLTLVESEKKLKELNTAKDRFFSI
                     VAHDLRSPIASLLTSLDLLTDPESDFSIEETHEFLLKLKESSETTYSLLENLLQWAKS
                     QKGEIKFNPEENSLNDLINENIKLFSNLSKSRGIHLSSQLEKNHFCFFDKNLIDTVLR
                     NLINNALKYTKENDSVLISAIEKDNKVIISVKDTGTGMNENTLESLFRIDVKHHSQEG
                     TRGEKGSGLGLILCKEFIEKHNEKIWAYSELGKGSEFLFTVPIVFR"
     gene            71648..72166
                     /locus_tag="PLF66_01740"
     CDS             71648..72166
                     /locus_tag="PLF66_01740"
                     /inference="COORDINATES: protein motif:HMM:NF024806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HMZ62784.1"
                     /translation="MQPDILHTYYQALMCDSEEQYENLIKDLSDVVKKNKENPFAYNN
                     LGLAYSEIGRLEEAEQNFAHSLKIQKHPTPYKNLGMMMERNEWLDRALTAFNHAIQLN
                     ELDPTLLRCRGSLYGKLGRYEEAIFDYTKAIALDPNFAYSYLERSNLYKAIGKDELAT
                     EDLSKYDVLSSI"
     gene            complement(72190..73902)
                     /locus_tag="PLF66_01745"
     CDS             complement(72190..73902)
                     /locus_tag="PLF66_01745"
                     /inference="COORDINATES: protein motif:HMM:NF017081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HMZ62785.1"
                     /translation="MAKQILKTFLIAISYVLGAKLGFFLAFLNSQVSPVWPPEGIAFT
                     AIVLIGKSAIPGIFIGATVANYMNNPHIPTSLLIGFGNTFGVIINYFLLMRLVGSERI
                     FDSIRNLMKFLYACTLPGSLFSAFTGVTSLLIFGFVPESVYWNVLATWCAGEMQGFII
                     VAPFLLSWSEPPDMRFPFYRYMELLVILVCTFVSSILAFRAKFPLAYLPFPFIVWASL
                     RFKKYAATTSIVFLSGLAVYKTVKGEGPFAVYLNEKVSLNDSLILLDMYIAVVTIIAY
                     FAVTVMRERSIAQNSAIENLKTIEKMKDIANQELERKVIERTLIIEEQKREMEKQIEM
                     AGRIQDSLLPAAIPDIPQLKIAVKYHPMMKLGGDFFDLRYNKHKNSFGFFLCDVSGHG
                     VPAALLAAMVKMSLTFWYEHTHKLMESLTFIYKSIYDKLGKNFITASLIHINLSRGKL
                     RTARAGHFPVMIIKNNGEVIRLNGRGKIIMALKEPDAEEVEYRLTPGDRIVIYTDGVI
                     DAKKADSSELFSEERMIQMILDSAKEDVYAISEKIFSSVTEYSGGKERLDDDLTFVLM
                     EYLG"
     gene            complement(73971..74372)
                     /locus_tag="PLF66_01750"
     CDS             complement(73971..74372)
                     /locus_tag="PLF66_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004458928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HMZ62786.1"
                     /translation="MKILIVDDNDRYANNLKAFFDSKKIETERAYDAKEGWEKFQRGN
                     YHTVISDITMETQTSGLFLVRKIFKSGYKGNIIIATTGFDFPGVMFFSKYILPFFAGV
                     GWMIPKVPLKRGEVIFFPTQLVNNKKFEESL"
     gene            complement(74369..75739)
                     /locus_tag="PLF66_01755"
     CDS             complement(74369..75739)
                     /locus_tag="PLF66_01755"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HMZ62787.1"
                     /translation="MPIKFRLFLILIIVNLCQTDTAIEKYQTAFLKKKGTVDETKVAR
                     IKQSLEGWLSYNYSSKVFPSIAVGVVKGSELVYSYKLGTTDRTNYGLGSITKTFTGTM
                     VMQAIEKGLIELDAPVSKYIPGLKLEKSELKSKPVTIRHLLSHTSGMPDLRYYQNPEV
                     IPSVITGLPFNITGQIYPAGYHYRYSNYGFMTLGVILEKVYNKPLKQIIEEEIFAKIG
                     MDASFASPIVQGAGGIQTNLIDMGRYASMWLNEGKSVTGVQVLKPETVNKMIQVQTPL
                     PAFAKTKKYCGLAWRTERDEEGVTTFFHIGGANYVAAWVQMFPKYDVAVFYLSNPQEY
                     DDNLMTQLVIMQWKLGDLASAIVGSEIPIHKTGDTYPSEEVYQRYVGNYKNPLTGKKA
                     TVFYKDAKLYFQPEGGGQIFLPPSTINVFGGPGGPGSYEFTYHPLTQAILGVSNYAGF
                     YERVYE"
     gene            complement(75729..76832)
                     /locus_tag="PLF66_01760"
     CDS             complement(75729..76832)
                     /locus_tag="PLF66_01760"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HMZ62788.1"
                     /translation="MLFLSPIVGNITPEDEGLSPDLTAKKLSTKKQEMEASEREIRKE
                     LVKLEKAMAALAQSAVSHNYIQGSSVAIYYKQNLVFEVNEVFKNSTPLSLASVTKPFT
                     SMAIIQLSDSGMLKLTDPIEKYIPEYAKQFPPIEGKPITVRHLLNHTSGIPYIGARQI
                     YAAGAKFMYSNYNYRLLANIIEKVSGQSYATYIRENIFFPLDMDDSMVSSNADGASGI
                     AVSARDLANFASVYLRDGKYKDQSIIQSSKIKKIFKAPDFMPKTSNMEYYGLGWRVTR
                     ENNLVKMFYHTGLWNGIFADLRVMPQTKSFIIQLCNPPSYKAAGFSSYQGQMNALATK
                     YLELLDKLPMQPETELATVSFLEDQEDLFPDAD"
     gene            77253..78017
                     /locus_tag="PLF66_01765"
     CDS             77253..78017
                     /locus_tag="PLF66_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008595394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62789.1"
                     /translation="MESSFKPKPILNRNELRQIKQSRTRVEGKKVITDDVRKLKSLKV
                     TPDLAPDEAINYYKEPIWIEYYIPKESRFALETKYLYILLIPPTPRPIDEILIKAQKE
                     DTFINLLELSNSKEKYAEVINKSYRNSLSILETISDNLDQYKESNNAEFLKIAVYLTE
                     TLMLTEPTLATLEVLGEYSVHNLIWLIKRLNKGGVLFSLEDRTISLLIKRRNEFFEEN
                     DYPVDDDFELLSALFFEQAYPHRGAEELQAQDFLLD"
     gene            78124..81318
                     /locus_tag="PLF66_01770"
     CDS             78124..81318
                     /locus_tag="PLF66_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004449462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HMZ62790.1"
                     /translation="MTFLHSIYDSIYFNYLSFGTLLSFIFTILLGIYLITLPGKSKST
                     FHLGLGFVFMGLFNLSYVFAAMIYQPFAAFHRWGSVGFILPGILHIIQWIFYFPEDSF
                     TKVRKTFQYTQWIIAIIVEIIFIYVSLHSNTKFHFTGHYWDFDAEWVSKVVGIVIFGY
                     ILLVPAVGIWKTVVVKTHERWAILQITIAIMVAAIVPAFMNILSRDGILDRGIFLTSY
                     VLFTVFGFFFVVIIFINETNDRTTFMAKIVGVTLVTFLLLMQGMSFASMQDKEVEYDN
                     IRIENIHRYLEGGTKHADMQYILDLRQENPEVQKTDYEDIDNLDLALIQQDFKNSLVY
                     EQIKELPVENFQTSLATLLSTTHEEFSGYKNSILEFVEANKGLSNEDLKANLFPYFEK
                     LNKLAFIATTKIGNFADKDFCSETTKYLKNNKDLIQFRDVIFVHLQDCNWNRSYLEPA
                     KLKREINKYLRYFKPAMTRHYRKSIDGSKHYVAYIHYNSAKKSITEIGFSYIAYRKYL
                     HPAAKSQIIILFIVLVIILGVFPLFFKGSLINPLMSLLRGVAKVNSGNLDVVVKVKVQ
                     DEIGFLSQSFNSMVDSIKQARKELQDYADNLEEKVKERTKEVQEKMEEVQKLKIQQDG
                     DYFLTSLLAKPLFFNANKSKYVTTDFIIHQKKRFEFRNKVADLGGDICITGNLKFGTP
                     EKFKRYTMAMNGDAMGKSMQGAGGSLVMGVVMNSIMARSAANKRILELTPEQWLTDVY
                     HEVNSVFKSFNGTMVISATVLLIDDESGEMLYWNAEHPFTILYRDEKANFIEDGLRLR
                     KLGLDSEYEFAVYKFQLVPGDIVILASDGRDDIDLTPNEPVRTINDDETMILSVVDEA
                     KCSILEIERILQTRGDLTDDLSILRIDYKINMPAEEAQSPMSLTPSFEEKEISNQINV
                     NEIYSQSKKLYQAGEIEKALELLANAYTVEQNNQKLNKLLGLLSLKGKDYDTAVRVLS
                     QYLSQDPDTDEIWYYLSIAQKKMGNYLSSLEAAKKVYELQPNNISNLVNLSDLYRLNG
                     NYIEAKIFSEKVLVLDSENKNAKKILNVLNSMNN"
     gene            81341..84421
                     /locus_tag="PLF66_01775"
     CDS             81341..84421
                     /locus_tag="PLF66_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008589283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HMZ62791.1"
                     /translation="MNLNWLHFSYYSVGVFAAFLFIFILTIFFITKKDKTAPTYWLAG
                     MFIGYDFMLLGYVLAYSISEPWGAYHRFITMCALFGNAGVIGFVYFFPRLDTPREAKI
                     VISLSLIIIFLAYFEFVITGSKLEKIYNFTAHFYTFDYGARQAVILLLTQLLPLSVII
                     RKTIRYSEYDGYFSKWLKKPAAISGYPLHLFSRFIVGWIKFFNPKGKDAHACKSFVKA
                     ISFFIVIASANLLNKAGIINYDLYSAIYVNVTLILIFYFFITYINNSPEPTTFMVKLV
                     GVSLITILLVLGYVGNITLSLSEEDYDNQRRAQIFGAKQNIVEGKYDELPEEIEYILA
                     KPAMADIFDKSYKIEFIKKPDVLTAEMINQGADRFKEGLIRETLTKLMKENKRDIKKD
                     KPTNEEYEEAINRFKKSRQYDNIANENLVDETNQKMNRLYRDAVILYTHYDFIKDNVK
                     YEIGFSYSEYRKHTHHNALKLIYIIFVTTVLVLITFPRFFASSLVKPLNNLLGGVTKV
                     NEGNLDIVVPIKVNDEIGFLSASFNSMVASIKQARRELQDYAENLEDKVAARTKEVQE
                     KMEEVQKLKIQQDGDYFLTSLLAKPLFFNANRSSSIKTDFIIRQKKKFEFRNKSADLG
                     GDICITGNLKFGTTERFKRYTMAMNGDAMGKSMQGAGGSLVMGVVINAIMSRSASNKR
                     VMNTTPEQWLSELYYEVDSVFKSFNGTMVISATILLIDDETGEMYYFNAEHPFTILYR
                     DACASFIETELRLRKLGQESEYPFAVYKFQLMPGDVVILGSDGRDDIDLTPNDPIRTI
                     NEDENAILATVEEAGGNIYEIENILKTKGAITDDLSFVRIEYAPVESQVSSIPETYQP
                     ITEKLEQPEIEIKEDAELQIDVAEVYQQSKKLYQDGQVNKALEVLMNAYAVEQNNQKL
                     NKLLGLISFKGKDYATAVDVLSRYLQMDPDTEEMWYYLSLSQKKMGKYLESLEASKKV
                     FELHPENVNNLVNLSDLYRLTGHFAEAREFSEKALALDPTNQNAKKILNYLGNV"
     gene            84425..85162
                     /locus_tag="PLF66_01780"
     CDS             84425..85162
                     /locus_tag="PLF66_01780"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HMZ62792.1"
                     /translation="MLIDQDSGILLRTIDSTNTYCKKKEVPIGYWVLADEQTAGRGRH
                     GRVWLSQGDEKIFFSAKFTLDKLESSISILSLLIGSAVLKTLQNYFPEQKNQLSLKWP
                     NDIYLGNKKVSGILIESESIEEKTVFIAGIGINLYGKEKLETSDYVSQKPIGKDFKQK
                     LIFSLIAYINEIEWILQNTNLLSNEIQWIYENSLLKNKKIETTDNGRNLSGIVTGYDR
                     NGFLIVKITDGNLVSLMDTGPDFKVGL"
     gene            85171..85944
                     /locus_tag="PLF66_01785"
     CDS             85171..85944
                     /locus_tag="PLF66_01785"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010575955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HMZ62793.1"
                     /translation="MLLVIDVGNTNTVFGVFYGEEEEPRFHKRTVTRRDRTSDELGVF
                     LKGFFKEENIDSNEISAAIYSSVVPGFNPIVSRMLEDWFKVNPISVTVNINLPFTITY
                     PRPFEIGADRLVNAAAVVRLYPGRSIIIDMGTATTFCVVNEHHEYIGGLIAPGLKISM
                     DSLIRNTAQLPPIVFQTPRKVLGDSTIESLQSGFFYGWVGLLEGIIQEIKREYGQDYK
                     VIGTGGLITTIHNSRKGLLDYIDPLLTLKGLKLLYEMNR"
     gene            complement(85957..86868)
                     /locus_tag="PLF66_01790"
     CDS             complement(85957..86868)
                     /locus_tag="PLF66_01790"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HMZ62794.1"
                     /translation="MTQRYTIIHRTRYSYDYEASESYSQVKISPLETLCQSVNSHWIE
                     VSPDVPMFSHRDYFGNLVHEFSVPFRHSYLEITATSDVTLFAPSHEPLNSLMKIKEAK
                     EWFDKYQLEFYDYLNPSIFVPITEEVRNFAKKILDPERRLTDAIIDLNQIFTKEFKYK
                     SGATNINTPIDEVIQKRMGVCQDFSHTMIAALRSAGIAARYVSGYIESYDPDSKQPEM
                     VGAEQSHAWLDVYIPDFSWFGLDPTNNMISSEKHMRVGLGRDFRDVSPVRGTFKGSGR
                     QSLEVDVKMRRAETKEGSRQATLQKPS"
     gene            complement(86865..89480)
                     /locus_tag="PLF66_01795"
     CDS             complement(86865..89480)
                     /locus_tag="PLF66_01795"
                     /inference="COORDINATES: protein motif:HMM:NF025761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HMZ62795.1"
                     /translation="MNWQMTSEQSNSFSVRTNQSKESFSEIKPKNSNGKYRPEYSEIL
                     PMLESQSVDRLRSINKRIEFVLKEQGITFGESRRGNYVERPWYLDLIPHIVNESEFSL
                     VERGVKQRLLALNKFLLDIYSSRKILIDKVIPKEVILADPNYLRDCQSVKVPHDVYLH
                     IGAFDIARSSDGSFQVIDDNVTIPSGISYAMTNRQVLRQQFPNIFEKASIKQIWDTTP
                     VMLSILKECAPTQDNNPMVVLLSPGIYNEAYSEHELLANKMGIPLVLPKDLIVKDNHV
                     FMKTVYGLSRVDVIYRRIQDYYIDPVSFYQDSVLGVPGLFSCVRYGNVTVANAIGSGV
                     ASSKSLLPFTDAIIRYYLSEEPILKSVPTALLSDPKFVEFVFSNIDDFVIKPKQGTGG
                     LGILIGRESTRAQIEDMKQKVLKNPFEYVAQELIPLSTAKVFTPDGNFKERYVETRFY
                     TFLGNSFHLSNCALTRVSKEESSLMVCNAMGGGSKDTWIMGKNDVIHRRSEILFPNRI
                     TRSLILSRVAESLFWLGRYINRGFTTANVLQVAYSSEIDILLGSDDPSYTSLIKTLSR
                     LTGSPVKKLFKSSEPWHVSFFKHAVADSRNPYSVKSNMNYAMNNAREIQNYLSNDMWV
                     SLRKLLEYLSELPGVEDSVNQNMTVDHLSEWLVGVVHYSQSFYGASLDTFSRQDILQF
                     IQLGRYIEHCSYIITVIKSTLQFLVKATQSDTDMPNLQPFIIVILKILNSYEAYQWNY
                     QSIFDPYLAYKMMITDKGFNNSLVSSLDKIKSILNAIGTDSGLTMHDEETPEYICDVL
                     ISRAFSFDLKDNLGKPSVNPPKIKKQDQFLYAKDEVKPGYWASHLKAGIELLGNKIMD
                     RYSNISSPTPFTVQL"
     gene            complement(89477..90271)
                     /gene="thiD"
                     /locus_tag="PLF66_01800"
     CDS             complement(89477..90271)
                     /gene="thiD"
                     /locus_tag="PLF66_01800"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004469955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HMZ62796.1"
                     /translation="MRDFPIALTIAGSDSGGGAGIQADLKTFLAHSIFGTTVITCLTA
                     QNPSGVSGIQEVSAELIEKQISAVTGYFPVAAIKTGMLFSKEIIDIVCELLKGKDYKI
                     VVDPVMVATSGARLLKEDAVDAIQKKLIPISSLFTPNLDEASILIGETITNEKEMDAG
                     VRKLYDKFGVPVLLKGGHLKESEYAVDYFYDGRTIYPLSSRFVPDFNPHGTGCTYSAA
                     ITANLAKGMTLIESISLAKYYIFEAISQSYTLGRTTTLNHNPITIK"
     gene            complement(90268..90621)
                     /locus_tag="PLF66_01805"
     CDS             complement(90268..90621)
                     /locus_tag="PLF66_01805"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020771942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HMZ62797.1"
                     /translation="MVVKPKKQEIKKDSFYERVYKAVKKIPKGKVATYGQIASFLGEP
                     RAARAVGYALNALKKQQEQEIPWQRVINAKGEISFKGDTIRAKLQRKLLEQEGIVFNI
                     VAQIDLTIYGWFQTK"
BASE COUNT        30245 a        16050 c        14255 g        30585 t
ORIGIN      
        1 aaaaacttac caatcctctc aagcctttgg atttttacga taggtttcat ttatgtttct
       61 ctgactgtta ctctaatggt ttcatcccct tcactcattc aagctgacac tctcacagga
      121 gagaaactat cgctcaatct agttttaact ttttttcctt ctcttttatt tgtgcatgcg
      181 attataccag cgctgattgg ttactttctt gtaaatgatt tcactttaga agtaagagaa
      241 actgtgcatg agaatttcaa tattacgtta cccgctagac atagaaaaat cggattaacc
      301 ttggcaatac tttttggtct tctaggacta tacccttctc taatgattat cattgatctt
      361 atgtccttaa gcaattctcc aaattcatat actcaattta tgaaactaaa tccgatcgag
      421 accatactgg tagattgttg tattgtattt atcagtatgg ctcttcttat ttttttaatt
      481 aaaagatcat tcacaaaacc aattgatcgt cttttaggaa aaatgaacca agtaagagag
      541 ggggactata caacacaggc tgcaatatta tcggatgatg aaattggtat ctttgctgga
      601 gagttcaata gaatggtgaa aggtttagaa gaaagagaat ttattcgaaa tacattcgga
      661 aaatatatga ctcacgatgt agctaacgtc attttaaaga acaaaattaa tctagaagga
      721 gaaatgagga tttgcacaat tctagttact gatatcgctg gctttacttc aatttcagaa
      781 aagctaagtc ctgtagataa tgtcagaatg ctaaacgaat atttttctgt tttagttaaa
      841 attatccaag agaataaagg agtcgtaaat aaatttatcg gagattcgat ctttgctcta
      901 ttcaatgtcc ccttaaatga tcctgatcat gcaatgaatg caattaacgc tgcgagggaa
      961 atacgtaata tgactacaaa acacaagttc ggaaataatt atgaactctc tactcggatt
     1021 ggtattaata ctggtatggt tttagccggc aatatcggtt ccaaagaaag gatggagtat
     1081 acagtcattg gagatgatgt aaatattgct gcaagattag aacagctaaa caaagaactc
     1141 aaaacttata ttttaattgg agaaaataca tattctttaa tcaaagataa aattgaatgt
     1201 gcctttatta aaaatgttca attaaaagga aaggaaaact ccataaaagt atatacaatt
     1261 catcattaat tcattgctat tagaaatttt ttttagattc tttagtatat acaataccgt
     1321 atatcgctta tttctgtcga tagtttgtaa atttcacgac ctagaaaata attaaaaaac
     1381 aaacatctaa ttctccctaa cttctctaaa aactgtcatt atacatatca gaataagatt
     1441 aaaatttcga tacatataaa ctcaattctt acttttcgaa aaaggtgcaa gttagattag
     1501 aattaatgaa tttacaagaa atgaatattt ataaatctat gtttgacaaa gtatcgtaaa
     1561 aaacgaaaag gatttttcat gcagaaccgc atactgattt tatttgcaca tcctcgattt
     1621 gaaaattcta gggtgcataa agctctaacg cattccattc ctaaatcaaa ttcaatcact
     1681 tttcatgatc tatatgatct atatccgaac ttcaacatag atattgagat agaaaaaaaa
     1741 ctcttattag atcataatat aattatctgg cagcatccat tttattggta tagttgccct
     1801 cctcttttaa aacaatggat tgatatggtt ttagaatttg gatgggctta tggtcccgga
     1861 ggaaatgcac tacaaggtaa atttattttt aatactatct caacgggtgg ggcgaatgaa
     1921 gcctatcagg aaaatggtag aaatcgtttt acgataaaac aattattagc accttttgat
     1981 caaactagct atctatgcaa gttgaaatac ttacctcctt tcctagttca aggaactcac
     2041 aggctctccg atcaggaggt caaagataaa tcagatgaat atgctacact attaaatact
     2101 ttaacggaag caaatttagc tgatataaat tttgattctt ttgcaaattt aaatcaatgg
     2161 atattacaat acaagaaggt ctaaaatgag tcatgatttt ttgatacaag caatagttta
     2221 tctaactgca gcaattcttt gcgttccgat tgcaaaaaaa ataggaatgg gatccgtttt
     2281 aggatatttg acagctggaa ttttaattgg tccatttgtt ttaggttttg ttggtcaaga
     2341 agggaaggac gtcatgcaca ttgcagaatt cggagtggtg atgatgcttt tcttaattgg
     2401 ccttgaatta gagccagaaa aattttggaa gctcagaaaa gtcattgtgg ggatgggact
     2461 ctctcaagtt gtaattactt cccttatttt gtttttattg ggccttctag ctggttttac
     2521 ttggcgtgta tcggtagcaa ttagtttatc tcttgcaatg tcatctactg ccattgttct
     2581 acaatcactc aaagaaaaag gaatgatgaa tacttcagcc ggacaaggtg ctttttctat
     2641 ccttctattt caagatatat ctgtaattcc aatcttagca atcattccac tactcgctgt
     2701 aagtcaagtc actcaagact cttcgcataa cagtcattcc cttctagaaa atgcaccagc
     2761 gtggctacaa acgttaatgg tcttaggagc ggttggatta gtcgcattgt cgggaagatt
     2821 tttaatagtt cctctcctcc atcaagtagc taaaacacgt ctacgagaat tatttacggc
     2881 atcatcccta cttatagttg tagctattgc atatttaatg ggattggttg gattaagccc
     2941 agcactagga acctttctcg caggcgttgt attggcaaat agtgaattcc gtcatgaatt
     3001 agaaagtgat ctagaacctt ttaaaggtct attgttagga ctttttttta ttggtgttgg
     3061 cgcttcaata gactttcata ttatttcaga gaatccattt cggattctaa tttatgtttt
     3121 agtaatctta ctcatcaaaa cagttgtatt atttggaatt ggaaaaatat ttcaaatgag
     3181 ccttgatcaa aatttatttt attctctagc tctatctcaa gttggagagt ttgcctttgt
     3241 tttattttca tttattggtc agttaggtat tctagatgca gcgtctaacg gcattatgat
     3301 ggctgtcact gcaatcacta tgaccctaac ccctatgatt ctattgactt acgaaaaatt
     3361 aattcaacct aaattggtta aaaataaaga agaagaaaaa gaagctgact caatcgagga
     3421 aaataattct gtcatactgg taggttttgc tcactttgga tcaacaatcg gtagatttct
     3481 aagagcaaat ggagtaaatg ctacgatttt agacaatgat tcggatcgag tagaactttt
     3541 acgcaaaatg gggtttaaag tttattttgg tgatgcaaca agattggatc tattggagtc
     3601 agccggagca gaacatgcta aaatttttgt atcagccata tcttctccag aaacaaacta
     3661 tgcaataggc gaaatgctga agaaacattt tcctaattta tctttctttg taagagcaca
     3721 agaccgtttt gcagcttatg aattgattga tatggggttt acaaaggttt accgagaaaa
     3781 tctagataca gctatcagag ttggaattga tatactgaaa gaaattggat ttagaagtta
     3841 tacagcaact agatcaggac aaaacttttt gaaatatgat gaagatgcat tgattggact
     3901 tgcaaaggat agacatgatt ctaagaaata tataagttct gttagagaac aaattgaaca
     3961 gatcgaaaaa cttttatcta ctgatatttc acagaaggca aatttagcag atcatgcttg
     4021 ggatagtgat ccattaagaa cagcggctaa tgaaatgaaa ccgctcaatc aatagaggat
     4081 ttaaggcaat gactggcttc ataaccaatc attgctagct ttcgaattct actccatcta
     4141 tattcaccga tgattccggt attttgaatt actcgatgac aaggaatagc atatgcaata
     4201 ggattggaac caacggctgt tccaattgct cttgctgctt tggttggatt tgctaagttc
     4261 aaatccttgc caagttgtga ataggtaact aatttagtct taggtatctt taataatgtc
     4321 tcccatattt tgatttgaaa atccgttcct ttgatatgta gcttgatttg atccgataaa
     4381 ttttctccat tcatgaatcg aaccattttc tcttgctcca aatccatctc tttaataaac
     4441 tttgcattct tccaaatttc atttaattca ttcaaagttt tatcaagatt ttcattataa
     4501 ttaagactac agattcctaa atcagtcgag gctaacatat attctccaaa gactgatttt
     4561 tggaaactat aatgaattgt taagttttcc ccttcattct tgtattcatc aagactcatg
     4621 ggaactattt gcattaccat atcgtgactt atattatttt tagaatctaa tttcagatta
     4681 agattttcaa ataacgtatt ttcattattt aaaacttgct ttatactagt tttactcaat
     4741 aaatttataa attcataaaa actaacgcct ccccattctc taaatatttc ttgaatctta
     4801 tcagaactca attgagtaaa agaagataga ttatttaaat cagcttgttc ttggttagac
     4861 tcgaatacat attctagaac tttcgcaata ttttcatatt gattcatagg ctcatatttt
     4921 caatcatcat caaaaattaa acttgtttct aagtttttta gatttttttt aattgagtat
     4981 agactctgat tgcttgacat tcacaaatcc tttaaccata aagtagtcta tgcaaagaaa
     5041 actaatttta tcatttttaa tctttatcgg atttattggc tctgcctatt ttatcactta
     5101 taatcgggtc aaaaaagagg ctatgaaact tccagataac tctccttcaa tttgttcaac
     5161 accgaataac gcattgccaa gagttgtttt agttggagat agtattacac acggtaaagt
     5221 atctgcaaat tatgtaagta tattagaaaa taattttcaa aatcaatatc agtttataaa
     5281 tgcaggtata aattctaatc tagcatacaa cgttctgcaa agactagatg agattattca
     5341 atgtaaacca acatttatca caatcttaat tggaactaat gatgtaaatt caacactcaa
     5401 tactaagaat caatcaagat atatttctga tatgaatctt cctagaaccc cagacttgaa
     5461 ttggtatgag gaaaatcttc gaaaaataat tgaacgatta caaaaagaaa caagcgcaaa
     5521 aattgcagtc ctttcattac cagttattgg tgaagatttg aattctaccg ccaatcaacg
     5581 tgttaaaaca tactcagctg ttgtcaaaat attagccaat gaatataata taaagtatct
     5641 tcctctgaat gaaaaacaaa acgaatttct agcgaaacaa gataatataa gtcagagttg
     5701 cgaatctaat tctgataatc ttatgattac ggctattgca tctcattttt tatttaaaaa
     5761 atcttgggat gaaatctctg agacaaataa atttaaacta gtaacagatt gtcttcattt
     5821 taatcatcta ggtgcttcgt ttatatcaga tttaattgct gacttcttga aaaaatcacc
     5881 tagtaaatag ctttattcat tgaaatgatt tctctatttt atttcgaatg tagaaataaa
     5941 aattaattat acaattatat gttttgtatg aattgaaatt ttttattatt ctttctaatt
     6001 tttttcgaat ttagccgcac atctttccct gttttacacc ctattattta tataccctac
     6061 gctaaaagta atttcggtgt ttggtatagg agttgaattc ttacttactc actttctttc
     6121 taagaaatct acttgtttag agtgaatctc caatattaca ccaaaggttt ttttctatgc
     6181 tcaaaataat ctctaataat aaaattacat tcccaagaaa taatttgaac ctggctgatt
     6241 ttagctcaac aattcttatt ccagaaaaat acgtatcact attcctaaga aaaatccaag
     6301 aacataaaag tatccatagc tatattagct tccttttaaa aaaatataaa ctttcaatta
     6361 aatggtctcg ttccaggata ttctaatatt acaactaaat accaagataa aggtcaaaat
     6421 cttaataaat ttgcatttcg cccaaagcca gaagattggg cagaactgaa actctatcgt
     6481 gtaagtttcg gtatgtcaat ctctgccttt ttagtatact tactaatagc agactctgtc
     6541 gattttacag aagccgtttc gaactactta gagagcgtcg gaatttcgcc gatccctaat
     6601 tttgatctag tcgctaaagt ctatcttttt aataataaga cctactatac aaccattttt
     6661 caatatcggc gaagatatat agaataaatc taaagatact actttttcct aaccttaaaa
     6721 ttcactagag agcaattttt atcgtaggac cagcgtttat agtctgatcc aatttttaga
     6781 actttaaaac cattcttttc taattcagag attatttcct catttgaata aaatctttgt
     6841 ttatgaactt ctctaaattc tttaaatcca tcttgatcgt tagttccaaa gtataagctt
     6901 gaagtaattt ctttattttt ttgatcatac atattttccc aaataaaaaa tgtatcacct
     6961 attgtttctc taaaaattcg atcatgaaaa ttctgaatta aatttttctc actactcaca
     7021 tcaaaaaaat acaatccatt atcagataga ttttgataaa ttgttttaaa atgggtgctg
     7081 agttcatttg ttgtcgttag ataatttaaa gtatcatggt tacaggttat taggtcaaag
     7141 gaattagaaa attggaattt tctaatatct ccaaccgtaa actcaatatg cggataattt
     7201 ctcttagcca cttcgatcat ttcattagat atatccaatc cgaccttgta agtggagtca
     7261 ggaaagtatt gaaatagaga tcctgttccg cagccaaggt ctagggctct ttctggagat
     7321 gaattcggga atcctgattt taaaataaat aaagcccatc ttttaaaatc tatatgcccc
     7381 ataacatgat catatatttc tgaaaattta gaatacggct gtgctactac catgcgaatt
     7441 tttaagattt gtaaaaatgt tcctgcaatt tatgaaattc atctttatct gtagtataca
     7501 aagtgatagg tgttaaggtc acatagccct tatagaagga ttcaaaatca gaaccatgag
     7561 agttaacgta tcctaactca gtgagtgcca agaaataatg ataaatatta tctttaatat
     7621 gagcgacttc atatctatcg ctataagttc tttgtcctag acgagacaat tttatctctt
     7681 ccaaatcatc tgagtattct aaagggaagt ttatattata aacaacacct ttgctcaaga
     7741 tatcaatttt attagaaata aatcttcgaa tatattcgga ttcttttgaa tattcataac
     7801 catcttctct atttccagaa ctcacggcga gtgaatattt accatgaacg gcaccatgcc
     7861 tagctgcacc aacagttcct gaatagtgaa cgtcatgccc catattaacg cccctattta
     7921 tccctgatat taccatatca atttcaggaa aaatgtttcc aaaaagacca atatttacac
     7981 aatcagtagg aaatccatct acagtataaa cagaatcttc tattttctcg acgtgcattc
     8041 tatgaaaaat agttagagct tgtgaagtag ccgatctttc tctcgaaggt gagattaaaa
     8101 agacggaatg ttcttttgag agagttttcc gcaatgcatg aatcccttta ctctctatgc
     8161 catcatcatt tgttaaaagt atattcaaag ttgaccgcct aattatagaa tagctgtaaa
     8221 atttactaaa aaataagaaa taagtaaaat aggaaaatat agaacaaaaa aataagccga
     8281 taaagctatt ctaaaagatc taaatgatcc tatgctataa acatcatttg catattttga
     8341 aaataaccat atatacgcac taacgcttac gccaattacc accataaata ttgagacaga
     8401 tggcatgcct ataaatgtaa acactacagc tataggacaa gaaaaaatat atattatatt
     8461 taatgatcga gctgatgcaa tcagagttgg gagatcgccc gactttagta aatcttttgc
     8521 ttttatatcg atagaatatg aaatataatt tgaaaaaatg gaaagaaaga aataatgtcc
     8581 gatcgtaagt aaaaacaaag ttccaacata atttgaattt atagatgaac tggcaaataa
     8641 cgttgctact gacaaagaaa aagacccaga caaaatacaa aaaaactcaa agaactttac
     8701 agttttattc attggactgt aaagtcttaa aaattcatct cgatctaaaa ataactcaga
     8761 aatcatttga atgtatagat tcatttttta tacctagtta ttcattttta atgcagcagg
     8821 aagtattaat aagattggac ttttaaagga tccaatttga ataggattcg aatttactcc
     8881 tagttttgtt cccattatat taaaaaatcg atcaaaagga ttttcctcat cttctaatag
     8941 atttagattt gtaacaccgc ataactctga tagttttgcc aatgcttcac gtcttccgcc
     9001 gatttcatct actagtttat tcttaacagc agattctcct gaataaattt taccttctgc
     9061 taacttttca atttgagaaa ctttcttttt tcttccttta gcgacatccg aaataaatct
     9121 tgcatatgta tcactcaaca tactgtcaat aattgattcc tcttcctctg ttgcatcttt
     9181 aaacatagat agaatatctt tatacttacc agctttatat actctcattt caactgaata
     9241 tttttttaat agaccagaaa tattggggct aatcgttatt accccaatgg aaccagtgat
     9301 ggttccagct tcggtaaaaa tataatctgc tgcactagca atatagtatc ctcccgaagc
     9361 cgcaatatcc ttcattgaga ctactatctt tttgtttttc ttttctttta ggtatatcaa
     9421 ctcatcgaat atctcttgtg aagctccaac tgttccgcct ggagagttta cctctacaag
     9481 gactccttta atttcattac tatcttctat ctctcgtaac ttcgcaagaa ctgtatctgc
     9541 ccctgtagat tgataagtcg atttccctga atgaatctct ccctctatct tcaaaagtag
     9601 agcggaatct gttgtatttt taaatacatt tacattgcct gttctggcga ttttagattt
     9661 tgcttgaatc gaaatatgta tcattgctaa aacagtagat gtaaacgtga atattaaaat
     9721 aaataaaagt agcgttcgat tagtagtcat tattcttatc ctactcgcca atctattatt
     9781 tatcccaatc gagcaaatta aatattttta tttactctga cattttaatt aatattttag
     9841 gctcttgtaa gttatgaaga aagtttttat ctctggcatc acagggcaag atggtgcatg
     9901 gttagcaaag ctcttgctca gcaaaggcta taagatttat ggtggggtaa gagataaaga
     9961 tagtcttatt gactggagac tcaagcaatt agggattctt gaatcaatag aattcatcaa
    10021 aataaaccta ttagatccag cctctgttga aaattgcatc cgtgaattac gaccagacga
    10081 attctataac cttgcagctc aaagtagtgt agcagaaagt ttccattccc cactcgagac
    10141 aagcctagta gatgggttag gagttctata tattttagaa tccattcaaa agcacagtcc
    10201 aatcacaaaa ttttttcaag caggcagttc cgaaatcttc ggcaatgccc aagaaagtcc
    10261 ccaaactgaa gaaacgccaa tcaaaccaag tagtccttat gcaatcgcta aggcatatgc
    10321 acactggatg aatataaatt atagaaaggt atttggtctt tattgtgtaa atgggatttt
    10381 atttggacat gagtcagaac tacgaggcaa agaatttttc tctagaaagg taagcttaca
    10441 tgtagcaaat tggtatcatg gtaaccgaca aattttggaa gttggaaata tcgacattga
    10501 aaaagactgg ggttatgcgg aagaatttgt attaggtatg tatctatcgt tgcaattaga
    10561 caagccggaa gatttcattt ttgcaactgg aaccaaaaca aaggtcaaaa attttattga
    10621 tagtgcttat ggagaaatca gcgttagtct tcaatgggaa ggagaaggag acaatcaaaa
    10681 agcctacgat aagaagactg gtgaattatt agtaaagatt aacccaaaat tttatagacc
    10741 aatcgaagtt aaaaccgctt gtggaaatcc tcaaaaagca aaagaaatat taaaatggga
    10801 acctaaaata tactaccata aaatcgctga gctgatggta agaagtgata tacaaaacct
    10861 gaagaagtaa cttagttata aaatttctag aatttttata aattattatc gtcctaagat
    10921 tatttgagaa tgcgaatgaa agaataaaat caaagacctg caataactta tgttgtagtt
    10981 cttatgaaac catagtagaa aaagctctca ggtctttcta acttactcta aggctattac
    11041 tatttttcaa tggtaatgga attaatcttt gaatcacccg atcttttata atgcaatgaa
    11101 aatagcgcaa gcgcaaggag agtatgtaaa aaagagaaga attaaaaata cttatctttt
    11161 tttatttaca atgaatattt ttcaaaagag acttgttatt atagatacta gtcatgtata
    11221 aaatccctag tagaaagcca tattacatat tattaatatc ttttattgtt ttttcaattt
    11281 tattctttta ctataaagat ttatacaaag aatttattat agagaaaatt tacacttcta
    11341 ttaattttaa aattgaattt aaagaatcaa gtctttcctt ttttccctcc cctggaatac
    11401 aattaagtga tgttagaata aacaacatag aggaatataa taaatataat gctgaaataa
    11461 ataaaatggg attttatttt tcttggaaaa ttctaatcgg cataattgaa ttaaatagta
    11521 tacacatcaa gtccggcaaa ttagaattat cccctcttct gaaagaaccc aacttaaatt
    11581 ctaaaaattc aatggatcca aaatatattc aaaaattaat tggatactta aaactggatt
    11641 taattgtatt ggattcaatc gaatttacac tcaataagaa tgaaacacgt tatgagttct
    11701 atatcaaata cctagcgata acaaatgatc atctaagaga agttggactc gcctttgata
    11761 taagtcacaa taatggaaga tttatttctg atacaaaagt tttatttatt aatagtgatt
    11821 attcctttca atcccttcaa attgagtcaa aaattaaatt tagtaacttt attctcaaac
    11881 ctttcaaaga ctattatagc ttagtgagag gggctaattt cgacaacaca tctctatctg
    11941 gtcaattaaa catctacaaa cccctactaa agaacgaatt taattttaaa acagatatta
    12001 acatcagcgg attgcaattc tctggagcac caatctatcc aataatcggt gtaagttcag
    12061 aactgatgta ttcaatcgag aaccaacaga tttacttttc aaacattaga gtgaactatg
    12121 aaaagggagc cattgccaat gcttctggaa acttatcctt cacaaaagat atctatctaa
    12181 atttaaatat aaacggagaa tatgctgata tctacaaagt tgtttatatc attgttagat
    12241 ctttggacat acgtatcagt agtaatctta acttttactc tcacatgaaa atcaattgct
    12301 ataaggcagt ttttgatctg tatgaattta aaaatgcaaa tcttgattta gatattatta
    12361 attcgctagt aaaaattaaa gtaaatcatg cagatactat ccatggccat atcagtggag
    12421 acggtagtat ccaagcaagt tctaatacaa gctataattt taatgtcata ttaaaagata
    12481 taaacactga agaatggatt aaaaagtata ccaccaatcc atatataaag ggagcattaa
    12541 gcgcaaactt atcattaacc tcaaatggca ataacttgga aaacttttta gaaaatcttt
    12601 ccgccaaggg aaaattagaa gttaaaaaag gtgagttatt aggatatgca aatattttaa
    12661 aaccagtatt cagtcttggt aaattagtga acatcctagg tccaagggga aagaatacag
    12721 aatttcaatc gcttatctgc gacttttcaa tttatagaaa aaacatttca gttcaaaatc
    12781 tcaaaatggt aggagtcggg attgatgcac atggatcagg aaatatcact ttggacagaa
    12841 aaattgattt cagaatttat gcaggacttg gcggtattgc aggaaaagca ttatatatcc
    12901 caattatata caaggggatt atgccagata acgtttccta tatagaccca gtttggatag
    12961 gatctgttta tgtaggagct acattgttcg gaggaccggc aggcgcaact gtaggaggtt
    13021 tcgctggttc cgccgtttct gaatatgtga acaaagcttg ggatggatta aagaaaattt
    13081 tttcgagaga ttaatttttc aaaattaaat taatccacaa aataaattct taaaatctgt
    13141 ttgtaaataa tgagcagtgg cttcatgtct ttttagaaat tgaaatttca acttgtaaaa
    13201 aataaaatac ttcgcaactg aattcataaa gaaatcaaac tctatatgat ctgtgagaac
    13261 agaatactcg ccatctgatt caaatctatg ttcatgacaa aaatattgaa aaggtccttc
    13321 tctttgaata tctataaatt tatgattctt ttcaaaacca atatgctctg ctttccatat
    13381 ttttttgata aatggaaata tttggacttc taaaattgcc tgtgttccaa tttcagaaat
    13441 agaggatggt ggttgtatta ctttcactga tggatccaat ccaactaaag tttcaaagcc
    13501 aattctatcc tcatgaaatt gaaatagtct ttcaacagga caattaaatc tagatttaca
    13561 ctcaaacact gctttcatat tttttctaca ccaaacaaaa ttttaatact atacgatctt
    13621 aagtttagag ataatcaaag ttccctctta taacaagcaa ttatttagac taaagacttt
    13681 atatcaaaac aagttaagat cagactttag gtttataatt tataagtata gtgtttgcct
    13741 taatacttca aataatattt attggatgaa tgaaaatctt tcattttttt ttattactat
    13801 tagtaagctg caaaaatcta tccatcccta tgatagcgga taacgctccg cagccgcatc
    13861 ataccgagaa tggattccaa aatctagatc caagtgcaaa atcgcaaggt ttaggaatgg
    13921 tattatactg gaaattagga aactttttta aatctcttcc ctctgtagaa ccaagcgatt
    13981 ataaaatcga aactacctta aacaaaggag aacttcttca aaagaattca aaaaacttaa
    14041 gtgtgacttg gattggccat gcaactgtat tgatacaatt agatggacaa aatattctca
    14101 cagatcctat ttggtctgag cgctgttctc cagtgagttt tgctggtccc aaaagatata
    14161 cagctccggg gatttcaatg gatcatttac ctcccataca tattgtcatc atcagtcaca
    14221 atcattatga tcatctagat ttacctacat taaaagaatt ggaaactaga tttaaaccaa
    14281 cattttttgt cggcttaaaa aatgggaagc tcctaaaaga ggagggaatt cagaatgtaa
    14341 ttgaaatgga ttggtggaac gttcaattag agcgagatat aaaaataact tttactccta
    14401 cacaacattt ttccggaaga ggactttttg atagagaaga gactctttgg ggaagttata
    14461 ttttagaagg aagtaaagag acagtttatt ttgcggggga tactggttat tttcctcaat
    14521 ttaaagaaat aggaaagaag tttccgaaca ttgatatagc catcttacca attggcgcat
    14581 acgaacctcg ttggttcatg aaaccaatcc atatgaatcc agaggatagt tatcaggcat
    14641 ttctcgattt aaatgcaaaa taccttttgc cgatgcatta caacactttc gttttaaccg
    14701 atgaagcact cgatgaacca cttgtaaaaa ctaagtcttt ctttgaggct aacagaaata
    14761 aactgcttga tttaaaaatt ggagaatcta aattctttta aattttgatt gcaattatca
    14821 aaatggttaa tagaatgctt ccattcaaaa acactaagga gtttttaaaa aatgaaaaaa
    14881 ctagcgttgg taatatctat tgttttggca actgcgtttt ctacatctag cctttttgca
    14941 aagtgctatg gctttagaga tgcagatata aaagtttgtg taaatgggga ttcaaatgca
    15001 gacagaaaaa aagcatctga aacttgcaaa aaagttgtgg gcgctgattg tgggggaatc
    15061 acaggttatt ctggttcttg cacaggaccg aaatgctatg atgatggtgg aaataaaaaa
    15121 agcaacatta aggtagatta attcgcattt tacaaatcga aatctcttcc aatttgtaaa
    15181 cgaggtattc taattttatt attaaattag aatacttcac tttgaatatt ttttatagtt
    15241 ataaaataga aatagactag attagatgaa tttgcggata atatcaataa accgcagtaa
    15301 gccactcttt ataactgcta ttttttgcag taaccatctc aaaaaacaaa tcttgtagtt
    15361 tttttgtaat tggtccgatg ctaccattac caatagctct tctatcaatt tcttttatcc
    15421 aagcaacttg aactcctgtt ccagaaaaga atgcttcatc acaaacatat agttcacttc
    15481 tagtaacatc tctctcgatc acttctattc caagctttct agccaaatca ataacagatc
    15541 tacgtgtaat tccttctaaa atggaagacg ttagggacgg agtaatcaac ctaccatctc
    15601 gaataataaa aatattttcc gcggatcctt cggatacaaa acctcttgaa tctaaaaata
    15661 ttgcttcatc acaaccattc tggactgctt cagactttgc aagagcagaa tttacatacc
    15721 caccagcagc tttggacata gtaggaatga ttgtatcatc aatccttctc cagctagata
    15781 ccattgtctt taatccactc tttgtatcta aatagtcatt gagaggtaga atataaattg
    15841 ctagctccgc ttttacatca tgaaaacgag gtgaaagttg taaggcagaa gtataaataa
    15901 aaggtcttaa ataaatattt tccttataac cacattcttt tactaattgg attattatct
    15961 tttctagctc agccggatca atggtaaatt gtaattgaac taatttagca gaattcagga
    16021 gtcgcttaat atgatctttc aatcgaaaaa tataaacatt gtctgtgtcc gagttataat
    16081 atcctctaat tccaccgaat acagatgttc catactgtag tgcatgcgtt tgtatactaa
    16141 ctaacgcttt ctcagaggga acaattttac cctcgaaata agataaattt ttataatttc
    16201 ccatgtgacc ctccatcttg tttaaaagta attgctattc cttaaacaaa aaactgcttt
    16261 caaaaatcca acacacaaat taaaaaacta tttaaagtga gttaatattc aaattttagg
    16321 aaaagcttat tttaacaagt aaatttcatt tggaatgatc aggttcaatc taagtctgaa
    16381 ttatcttctt tcctaattaa tttccttgaa agaaagaatc caaagattcc aacaatgagt
    16441 ccacctaata ccatggaata attattatta tttcctttgt ttagatatgc aagcaaggaa
    16501 ctttcttctt caatatttga agatactgga agttcatagg atgaatctgt ttgttgtagc
    16561 gacaaaatac cgttatcctg tgctgtaata tttagattat cagagcttac aacttttact
    16621 agaatatatg ttttactaaa accataaaaa ttctgtgttg aataattacg tatagcagta
    16681 tcttcatgag taatctttgt tacttttgat gcgtagttag aatctgtcca agttaaatat
    16741 aaatcactgg tctctctttg aagatacaat tcacattcgt gtccaggaat attataaacg
    16801 ccatagacag ctaaaatctt gtatttagct ccttgagttt gattcgttat actaaaataa
    16861 aaattattag atgttttatg atcccaagct aatgcactga tttgtccttg ttctttgtct
    16921 ttaaatgaat tttcttttat acttaaatcg atttttttct cactcccttt tggaagttgc
    16981 tgtaatggaa tctttgaaaa ataagaattt gaatcacaat agtttgctgc tgctatataa
    17041 atgatttctg aatattcttt tttatttttt tcatcgcgaa tattcatttt atagactgaa
    17101 gtatgtttta aagaaacact tggaacaaaa ataatttcat ttttattcac atcttcgata
    17161 taaccctcga tttgtgaatt atagttatta gcctcaacca aagagactgc tgcctgaatt
    17221 gtattttcat tgcctaccag ttggactttt tccttacttc ctaatgtaag ataaccattc
    17281 tcaagtatga tttttatcct accttctttc acttcgatca gagggtgggt tgcgatccca
    17341 gagaaatagg gagaccctcc attgctttgt tccaatctct tctcagagag attaaattta
    17401 tttgtctgat tagagtcctt taaatcattt gctgcgtttt ctttatctat acagctattt
    17461 aaaaataaaa tgaagattga taatagtaat gaatatttca tagatcttcc tcttgtctat
    17521 tccatatctt attcttgtaa agttaagata catctaaaaa atagactagg taatttttta
    17581 ttttatttca atccaaaaat aaaataaaag aatctactct gctaaatctt tactccagag
    17641 tccataacga ggttcaaaat cataaattaa gaacttttca ggattaaaag ttgagggagg
    17701 attccattta tcaggtattt ctacagcttt ataaagtcgg tctaaatatt gctctttaga
    17761 aatctcatac gcacctaaat tccaagtaat ctcattcaat tgctgtgtat caaataaggt
    17821 aaatccatct ctaaataaag cttcaaataa ataggtaagt gctacttttc ctgcatcgga
    17881 ttcagtagaa aacatacttt ccccagcaaa gaactttcca attgctactc cataaacacc
    17941 accaaccaat tccccttttt ggttccatgc ttctacacta tgtgcataac cctttttatg
    18001 caagtctgta tatccccgca aaaaaccctc tgtaatccaa gtgatttcat tcactcgatt
    18061 cgcgcaaccc ttcatgactt cagagtaggc tttattgaac gttatattaa aactattttt
    18121 ccgaatcttt ttgcgaacag ttttcgaaac atgcaaacca tcaatttcaa aaacagctct
    18181 cggatgtaaa caataccacc gaatcggatt ttgtgaccaa ggaaagattc catgcatata
    18241 ggcatagagg agtctatcag atgaaaagtc cccgcctatc gcaaccaaat cgtccatttt
    18301 tcggagtggg tctccaaaaa atttatgaaa atcctccata ccactcatct aaaatcattt
    18361 actccaacta aagaattttt ttgtaaatgc gttttcgtat cttcataaaa ctctaacatg
    18421 gatcgatatg cggtaggtgt taacttcaat ggttgatcat tttttaaata tttttcaaca
    18481 ggtaggacgt tatattgaaa tccatttttt tcattggaca catagacaaa aacaggaatg
    18541 taatttatat catgaattag aatctgtggt ttgcccataa tacttttctt tgttaatcta
    18601 aaattgaata tgattccgcc attcgtatat ctttttattt ggccagaaac aaaatttccc
    18661 aatgaataga ttaccattct ttgttttttc tccccatact tatcttgaat ccaatccacc
    18721 tcgtattgtt gtagaacatg tggatgacca cccaatacaa tatctgcccc ttcttggaat
    18781 gcaaactcaa ccatttcttt ctgaaactta tcagggtatc gtgcatattc tgtgccaaag
    18841 tgataagcaa ctaatactag atcaggcttt gccttgcgag ccagttcaat atcctctcta
    18901 attctatctt tttctataag attgaccact ttatcttttg gtattgcgat accattggtg
    18961 ctataagaat aactcaaaag tataattttt atatcattta tcttgactgt aagcaatcga
    19021 ttcttttcaa attcatctaa cgatctatag gttcctaagt gcaagattcc aagctgatct
    19081 aatgtttcaa ttgtatgatc aattcctttc cttcctgtat ccacagaatg attatttgat
    19141 gtagttaata tatcaaaacc tgtatccttt aatgcttttg ctaattcatc tggtgctcca
    19201 aattgcggat aacctgcaaa caattttgaa tcacctggta aagtagtttc taaattacca
    19261 aatgcgatat ctgctttaga aattgtatct tttacttctg caaatacggg atgaaaatta
    19321 taacatttgc attgagaatt ataagctgag gtaagctgag tagcgtgcac cattatgtcc
    19381 ccaactgccg caaactgaat cgatttttca tcgggagata caaaatcttt aaaagaaggt
    19441 gtcgcgcaat tagcaagaag taataataca aatgtattta cacttattaa aaattttcga
    19501 aaactataca tatttttatt ttttattaaa tagaattcct tcaatgcatg atcaaaaatc
    19561 aagacatact cggatatgat agcgtgaagg ctttattgaa aactatgtgt tacagtttta
    19621 attcctaaat agatttgtat attcaaaatt aaaattgttt tcttaaaaag tctctccttc
    19681 actcacctag cacaaggcag taaaggcgaa aaacaaggaa attcgttcga acttccttac
    19741 tcaaattctc agtaaaaatt cgaacaatca aagaatcgct tacgttactc ccaaaccaat
    19801 ggactgattg ttacgaaata gaatactttc tgttatccta tttatctatt cctttcttct
    19861 gtaatgttag ttattaaaaa gttaaggtta aattcaccat ataagtccta cctggttgtg
    19921 gaagtataga agaatcgtat ccaagagatc gtgcataata ataattaccc gcattcgctt
    19981 gtcctgttcc ggggtgaaag tagctcgtat taaaaacgtt cataatctta agagtaaaaa
    20041 atccataatc cttaaaattt atagttaatc ctgcatcaaa tacagtatat ggatccaatg
    20101 tcttatcttg ctgttgcact attcttcttg aaacattgtc cgtatcaaga gtagataaag
    20161 gtccactatc gctcaatgga tttctagaat aatattctct acgactcaca taatttcctc
    20221 taaagttaac aatgaataat tctttaatag gaaagttaat ccccatatta attttatgtg
    20281 gtgcaatatc tcctaaatta gtatattctc tatttcttgg aataaattta gcttcatttg
    20341 aatataagta ttcgtaatcg ccaagaggag ttgttccctc ttgccacttt tttagattgt
    20401 gatcataata tgtatcagct aacgcacgag tataagtata gtataaataa aaattcaaag
    20461 gagcagaatt acttaaaaaa ttttgaacat tccatctaaa tttatattca aagccataaa
    20521 ttctcctcct acccgcattc tgaggagatt cttttatcac gtcttcatat cgagaataat
    20581 aaacggaaaa ttcatttaag gtttgcttcc cctgcgtcat aaatattgct tcagtagtct
    20641 ttgctttttc cggtcttaat tttaaatcag cagtccttcc agaataacct ccaaaaagat
    20701 tcaatggaga tggctcatta aatgcctccc catataacaa tttaaaagct gtagcatctg
    20761 taattttata aataccggta attcgaggat tcaaggctct tccatagatc gagttttcat
    20821 catacctaat tccaggagaa aaacgaaatt tgcccaaatc aataatacta agtaagaatc
    20881 caccacgatc ataggtgcct attgtattct cttctggcat tttatttttg ggagaaggac
    20941 ctttcaaaat aaaaggatca ctactttgac ctatggcata accatttgga aagagtttat
    21001 caattccctt atttacgttt ggatttaaga aatctattga actaacatat gaagagcccc
    21061 accagtaacc aggaatatca taggattttg ttaacttttt aaattctact tttagtcctg
    21121 ctaatatttt aaccttatca cttattttgt attccacatt ctgatttact agaatacttt
    21181 tactaatttc attccaacga gtaaaactaa tataggaaga ataagatttt gccccatacc
    21241 cgtatccatc tgcttccgcc caatcgccat agatacgact atctcgatat acagctagag
    21301 aataggaact aagtttactt gtgatatcca tttgattctc tgcataaacg ttaactccag
    21361 actttcccca catagcagtt ggctgtgctt tgtcacccgg atactgagaa ccataggctt
    21421 catatttatc agtaatatta gctcctattt ttaatgtttt ataagtagca ctaattattg
    21481 ttccccaatt ttgcacggga tctgaatatt taccaaaggg tttaccacca ctaccataga
    21541 acaacaaagg tccccaaatt tgtggattat ttatccaata ttgtgtgtta taaccatagt
    21601 tggactgatt tttatttgca tcatttcctt cctgcttcct tgctgagaga gctagactta
    21661 aatctccaat ttggctcgtc acccctccat cggcgaccca ggtattccca cttccataca
    21721 taaaactagt tttacccgca atggcttttc cgttattttc tttaccatct tttgttatga
    21781 tattgattat accctgaaat gcattgggac catagactgc agaagctgga ccatatatta
    21841 cttccactct cttaatgttt gagattggat attggcgacc tggagtggca acttgtgccc
    21901 ataaatcatt ttcaatgact ccattgatca taagtaaagt tctagaggta aaaggagtgc
    21961 ggtatcctct ctggtataaa acagcattat ccgttccctg ttgaccaata taatcaaatc
    22021 caggaagatc tttaaaaata tcatctaagc tagtgtagcc tctattttta aaatcctgtt
    22081 ctgttataac catcgtggaa gctggaacat ctatcaaact ttcctttgtt cgggatgcag
    22141 ttacaacctc agtgtctcta agaagcttaa aaacatcctc ggttgacttt gtttcaacgg
    22201 ctaacactgg taatccctga tcttttccta tagatgttga gatgacatat tcatcaacat
    22261 tctcaggact ctctttacgg gctgtattaa atctcacttt ttgtgaaatc ttttttgtaa
    22321 atctatcaat tacaatatta tcttcaattt taatttcctc gggaggaaga ttcacctcac
    22381 ttacgtcacc aagagaatct gaaagattca aagcatcaat aatatttccc ttgtcaggat
    22441 tataaatatg cccatataaa attaaatttc catttggttt acgaccataa aatccactta
    22501 aataaaattt tgcgttattt tctttcgcaa cttttaaatt atctgaattg cttccattta
    22561 taattctaac ttcaaagcct ttggctgtta gttcattttt gagattttct aaaatggaat
    22621 cacgatttgt cgcttcctct tttgatttat aaggtaaaaa attacttata taaataatat
    22681 ttgaattagc accgtctcca acgattgaaa gaagaccaaa aaaaaataag gggataacta
    22741 ctgttttccg cagaaactta aaaaaaaaca tatcaattcc tattctagtt ttctaacacc
    22801 tatcgttata cgaaaggatt tacaaagact cgttaaactt atctttaatt acaataaaaa
    22861 aataaataat tggaaatttt tttcggttaa atcgttgtta aatgataatg cttttaataa
    22921 tttgagttca aaataatgaa atagaaaact tctaacaaat aaaaataaac tgaatattaa
    22981 ttgaaaatgt agattttatt gatataaata atttatcact cttaaaatca aattttagaa
    23041 gaggataaca atatctattg attcatgcag ctaactagaa taatccggtg aaaataacta
    23101 atcacatgac aacctcactc aatccttctt tcacaaaagc aataatagat tcgaataact
    23161 cacgagtaaa atatgatttg cttctattca aatcagaaaa agagaataga tgtaaagaga
    23221 atgtatagat agcattccac tcttcccttt tgcaaccaga ttcagccaag ttaggacaga
    23281 tgtatttttt atttgaaagt aattttgaat attcctaatc cgatctcttg ttctacaaat
    23341 ttcctatttt gcaataaact agacagttat agaaattaga tcattcaaga catggtaatc
    23401 caaacaggaa ttactgaagg ataaacgtta ttatcctcaa ggaatttacg aggagaggaa
    23461 ggaaaaattt gggaaggata aagtttagaa aatcatttca tacaaaacgt tgtatgaaat
    23521 gatttcggag gattttaatt aaccagcagt aactgccaat tcaactcttc tttgaccagg
    23581 atcgtttgga ccaacaccag gagctggctt ttcagcacca acgcctattg agtttccaat
    23641 ttgttcatca ttcacacctt ggctctttaa gtaatctttt acagaatcag ctcttttttg
    23701 gctgagagct ttattgaatg caggatctcc agatccatca gtatgtccag aaatgttaat
    23761 ttttgtatca ggataagctt tcacagattc agcaatctta tcaagggttg cttttccaga
    23821 tgcaggaact tcagctttac cagcatcgaa tttaatagct tgttcaccta gttttaaatt
    23881 taaagcttct gtatcaccat tgctaccttt tacttcagca acgccaattt ttttagattc
    23941 taaatcttct ttgatatcat ctttcattag gtcaaaatat agaccaacgc ctaaaccaat
    24001 tgcacaacca gccgctaaac caacagcttt tcctttgttg cttgattttt tctttttaaa
    24061 tgcagattta actacatcat caattttgcc accagacatt tttgtcttaa ctgcttcatc
    24121 ataagcagcc ccagctagaa gaccagctaa acaacctgtc acagctccaa taacagttct
    24181 cttgccaatt gactcacacg atgttacaaa aaaagaaact agtaaaatca caataaatgt
    24241 attttttttc atattttact ccttgcaaca ccaatgatat gaaaagggat ttctggtcaa
    24301 ttaaaaacaa gttgcattct tattgttaaa aacgaactct gattctagtt atgaagatat
    24361 caatacgaat gagtatcctt tgcagtctct ttggattagt aacaggaatt tttgtaagta
    24421 acaatgctac aggagaaggc tatgaaattt tcccaatctt ttcttttgta gcttcactca
    24481 tttgttcctt tttactttgg cattatttaa ttgttaaacg aaaatccaat tccatactaa
    24541 tgggaatctc tgtggggtta atcattgtta tcctctctca ttatttcaca tggtatttta
    24601 tggcgttata tttttatatc tgttcttcct tttcagtaaa atgtctttcc tctctgggcg
    24661 agaagacaat gaacccgatt caatccattt atcttcttgt tccctacact gtgttgagtc
    24721 tcatcatcgc atggataact cttcctctcg gtggatttat tggagcgatt ggagttaagc
    24781 ttcaaaataa taccaaagct ttggaaaaaa attgacttgc aaaaataatt aggtcaaata
    24841 atattttcct ccctatctaa taggaggaga ttactgtgaa acattttttt atcgtattga
    24901 gtttattatt cgctttcaac ttactcgcaa cgggagaggt agaaggcacc tacacatcta
    24961 aacaagagaa tgccaaggaa atctacgagc taaatctaaa tatcgaaaaa gatgacactg
    25021 gtgaagatgc gtattccttt catttaacaa aggcattcca agaagaatcc ggaaagaatg
    25081 aaacatcgat tattggtaaa tataaaattg ataagaaaaa cattattctt tatggagaat
    25141 cgattacaga caattcgaat ctagagaaca aacaaaaata caaaggatca cccatccgag
    25201 ttcatgttag aagcgaagat aatcgaacga ttctagtttt aagtgcattt ggaaaaattt
    25261 taaaattggc aaaacaatcc taacagtgag aacacgaaca aaatttaaaa tcatactcct
    25321 tctctgccta ttttatgtag gcagagttta tgcggaagac agagaaattt tagaaatacg
    25381 aaagtattat aaatcagttg aatcaaattt aaaaaactta actcaatcca aaatagacta
    25441 tattcataaa acatcacatg tagatgaaac agacttgtta agtgaaacca tttactttta
    25501 caaagataag aatcaaatcg tcaaaattgt aggagacaaa gtctttgatt gttccggtag
    25561 tgtaaatgaa ctaacgtata aaaataaaga tttactattt atctacacct atgattggat
    25621 gggttgtaat agagaagcag tcgctaaaga atctagatat tattttaaaa ataaaaagct
    25681 aattcgttgg ttagaaggca aagaaaaaat agataactca aagtttcctg caaaagaaaa
    25741 agaactacta aaactctcaa acttctatta tttaaaatcc ccaaattagg aagattaatt
    25801 atttccgttc aatacgatta tggttcagag aaaatcgtct taatctctac tcctttatta
    25861 cccaaatcgg taacgattaa actgactgtt ccacccgatt gaacatatgt ctctgtcact
    25921 gatacattgg tgattttccc attcttatct cttgaaagat caaaatctat ttcgatatca
    25981 gcagattttt gtttgtctgg tttaattgga aatttatacc gatcaaaatt aagaaggaaa
    26041 caaactccaa gtaaataagc ctcttctatt ctcgcaagag gtaagataag agtcgatgtg
    26101 gtaacagcac tatcgtctgt tgattgtgtt acctttgtat ctttaggtaa aaacttttct
    26161 gcatattgct ttagagtaat cgtgtttcgg gagggaagag ttgcaacagg tggtggatat
    26221 aaagagagat acccatcaaa taaatatcct tctctatcat ctgcgattac ttttacccaa
    26281 aatcctttta taccatctac atccaattct ttatctgtaa tttgtctcac tagagccatt
    26341 gttccaaatg gaacagcctg tagaaatctt ccattcctgg aaggcttatc tcgtagattc
    26401 aatcctgatt tagataaaac atataatcta tctcctggtt tataagttat agaaggttta
    26461 gaaaaaagag gaaaagtaaa cacaacaaaa ataatataca atatggatat aaatcgaatc
    26521 ataagaagac tctatctaaa aggaataaac tgtcactcta aaatatttta actcaaccca
    26581 aaagactcag ttttgatttg ctcaaataat ggctttcata aggatagaat ggatgcattt
    26641 ttcaaaaacc aaactcattt tattcgtaat atttttttct attacttctc atctaatatt
    26701 taactctatt atcctttata cagaaaaaac atcctttatt tggaatcaaa taggaaatag
    26761 aaaaatcgaa cctaaaacgt atgatcttgc aatcttaggc gacagtcaac taatgagtgg
    26821 agtgcaccca accttattaa aaaaatatct acaaacagaa agtaaagatt gggaaattct
    26881 atattatcca agaccttcag agcagccaga aggaatatat caacttcttc atcaatttga
    26941 aaagtctgat gttaaaatca aaaatttaat tgtaaatatt tcccctgtga ccacttcaaa
    27001 aaatacgata gtcgatgcac ataagtcatt aacacaaaat tttcaaccat tttctacaga
    27061 aataataaaa gatctttctt tgaataagtt ttatttaaaa aatttatcag gaaatgtata
    27121 ttatctttta ctacaagttt ttcctttgct aaaactaaac ggaaattttt caaatgaagt
    27181 caggattatc cctggctctg aaggaattca gaacgataaa cacattgaat cctatctaaa
    27241 tgttggcttc tttggtaatt taaatttaaa taaagaaaaa aacaattttt tatccaatca
    27301 tttgatcgat caaggtttct actttgaatg gggaaactat actacttata caggcgattg
    27361 tattctaagg caggaatctc taacgcttcc ctttggaata gaagcagctt ttctaaatcc
    27421 aagaaacgat tctctggaca tgtggaaaaa aattggagaa tattcaaaag aaaaaggaat
    27481 tcatatatat tatctttata ttcccttctc cccagaggca gaatccaaaa ttggatctaa
    27541 aaagaataca tcaccaatcc aaatcagttt aaactctata aaagctatct tcggaaataa
    27601 aagtgtttta gaaatagatt caaatctatt ctcaaatgaa gatttcagag attacactca
    27661 tttaaatatt tgtggaatga tgaagttgac aaaacaatta gccaaatcat tctaatcaat
    27721 agttttatgt atatccttcg taaattgatt tttgttttat tgagcttctt catttattgc
    27781 actaacgtaa atgatttata ttatagcgcc ggtgaggcaa acgcaaggat actggttgct
    27841 tttgcgatta aagatgcaga atgtaaatat actcatcgag taacagcctt tatccccgga
    27901 gaagcaagaa aaacagaaat caccgcatgc acacaagcaa tcctaacaaa actttgtgga
    27961 gaatggaatg taacagatcc aaccccactt ctttgcaaga caatcaatta tagaaacaac
    28021 tataaactca gagggatcta gaatgaaatt tgatttacaa caaacagaga tcatgcttta
    28081 tatttctctt gttatcgctg taattatttt aattctgttc tattttcgat ctagtcggaa
    28141 aagaaagaaa atattccgcc caatcacaac agaggataat agccctaagc cgcaaaaaga
    28201 gcaatccatt ctagaaatct tcgactatct tggaactaag atcgtgcatg aaaatggaac
    28261 ttacacagtt aatcataaag gaaaagttag tttttataaa tcttgggaac ttgttccaaa
    28321 agaattccaa aaaatggtta aggaattgga caaccgatca ggtcttgcta agaaaaatga
    28381 tgattacttt ttagaagtta taaatggaat ctattatcta accatgccag atggttctat
    28441 aaaaaaatac aatcgcttga gtgatgttcc cgcatacata agaaaggctg ttggcaaatt
    28501 ataatttacc gctatttctt tttaggttgt tctggaaaag ttaacaatac atcaaatcga
    28561 tcatttaatt cttgtttata ttcatcctca tcataaaata aaggttctac ttccccatag
    28621 gcttgatatg tataacgttc aaagtccata tctcgatcga acattttttt aaacaagtct
    28681 ctagctctcg cctctgttcg cttcatattg accttatcct caccaggttg tttcgaatgt
    28741 gtttgaatct gaactctgta accaggataa agtgataata attttttgat ttcattcagg
    28801 attttcgatt cctctttttt taaagtagat tcaggaatcg atattctaag tttatcttct
    28861 tttcctgtta ctaaaatacc tgtggaaaat tcttttactt taaaaaatag taattcattc
    28921 aactcatttc taaacgaaaa atgcagttct aaattactaa gcgaacctat taaaaatccc
    28981 tgatctgtat aaccatccca tacgattctt ttagctggtt gcccaatccc tttccatttt
    29041 tttataactt gagtttttac tgtatcgcct actggatatt tttgaaggat tgtcagtttc
    29101 caagttttaa tggtaaaatt ctctaactct ggaaatattt ccaaatagtc tgaagtttca
    29161 tctccatctg gagtaaaact tttttcagac aatttaaagt aaattttttt atcctttgta
    29221 ttcacagtaa ctttgcgctc gggtgatttt agattcttag atccaacaac acatttcaat
    29281 ctatagaaat aagtcccatc agaagctaac ttaccatttg tattttttcc atcccattct
    29341 attgaagtag ggagcacttc acctgaaaaa tttcgaattg tgttttcttt atttccatct
    29401 ttttcatata tattcatttc ccattttgaa ataaattttt catttgcaat gggtttaaat
    29461 ataacagtct ctttcaattt gtcaccgtta ggcgaaaata tggaaagatt tggctgaata
    29521 tctatattat ctgaactttt acttaaatat agattttcca attgtgtagt cattttattc
    29581 tgagaaaaat cttcccctgt caattgataa ctatatacgc catcatcttg taaaattccc
    29641 ctatcgtcct tgccatccca gtttaatatt ttgggaatag tccttgtctc ccattgaaaa
    29701 gttcgaatcg gttcattttg ttcattataa attacaccat tccagcgatc agatgggtcg
    29761 gcggatacat cttgttttat aataagctga tcaaagattt tatctccatt tggagtaaaa
    29821 aaacgattct cggtcgaaag tttcgcatag ggagcaattg aatctaaata aatagaaaca
    29881 ggtttagatt taatttccga tttgtctgaa aagaaaattt tcatctgatg aatataacga
    29941 ccatcgggca gcatttttcc cacgttgtca tttcccagcc attcaatttt ttcaggaatt
    30001 gttaccttta atggttccag ttctttatca tttgaaaaca aatatcttaa aaaatttcct
    30061 tttcttttcc ttcttagatc tgcagaaaat actttaataa ctttgccatt ttcatcagcg
    30121 atatgaatct tccaatcaac aggagttttg gagccactat atttaatctg aaaaaccatc
    30181 gtatcattta tatcatcata atttggagaa aaaaatactt taggtgtttc taaaataaga
    30241 gaatcagaat cttcagaaaa aataggaata ataaataaaa ggataatcgc aataaaccgc
    30301 aacataactt aaccaaacag aaattttatc gctctagata catagccgta aattttttta
    30361 gaataaggaa accaccaaag ttcatcagaa aatctccaga accatcgatt ataactaact
    30421 gcctgaacct gagaaaatgc tctcagacct tcctttccat gaactcttcc tataccactt
    30481 ttcttaaatc caccgaatgg caaatcagat atcatataat ttgcaaccac atcattaata
    30541 caaacagaac caacttgtag tttatgagct aaggttttag cctttcgtat atcgcttgtc
    30601 caaattgaag cactcaaacc aaattcagat gcatttgcta gagccaaaac ttcctcttca
    30661 gaattaaatt ccattataga aatttctggt ccgaatgtct cttctgtcat aattttcata
    30721 tcatggttaa ccccaatgat aatagctggc tcatgataga gtcccttctc ggataataag
    30781 cgtttgcctc ccgaaacaaa aaaagctcct ttcgcttttg catctcgata gtgagactct
    30841 aatacttcaa cttgttttgc aactgtcata gatcctacac tcgggaactc ttcttttgag
    30901 ccttgcttta ttttaccgta ttcttctttt agcaagctta tgaattcatt gtagattgct
    30961 ttttctacat aaatcctttc tacggaaata caagtttgcc ctgcatttgc aaatccaccc
    31021 caaagtgcac cacgaacagc acgacgtaaa tcagcatcct taaataaaat catcggatct
    31081 tttccaccca actcgagaac aactggtttc agcatacgac cgcacgcttc tgcaatggat
    31141 tttccggttt gtgttgaacc agtaaaacaa atcatatttg tattttctga attcacaagt
    31201 gcttcacccg tctctttcat gcctgtcact acctgtaaaa taccatttgt caaaccagct
    31261 ctttgaaaaa actcaagtgc tttgagtgtt gtgtagggtg taatttcaga aggtttgatg
    31321 actaccgtat ttccagccat aagtgctgtc atgatcggag taagacttaa aataagagga
    31381 taattccaag gagaaatgac tccaacaacg ccatatggat gataatttac ataacagttt
    31441 ttgtttataa ataagccgcc agatctcttc tctgaggata ggaatttact gccttttctg
    31501 gcaatatagt caattgcttc taaagtcgta aaaacttcga gcaatgcatc catctttgtt
    31561 tttccagttt cattgcaaat acaggaaacc atttcttctt tattgtttgc taaaaatttt
    31621 ttgaactgat ttatttcctt agctctttcg gatatcgttt tatttgccca aataatctga
    31681 gcttgcttag ctaataatat tttttcttcg accatttcag ccgagtaaag aggcaattca
    31741 ccaattttcg ttaatgttgc tggatttaaa atttcaaaac ttgcattctt ggatttattt
    31801 aattttgaat tggaagaatt atttttagac gagaatgttg ttttattttt tttcactgtc
    31861 tttttcatta ttcttctttc ctacaacaat gattcggtct ctcaagctaa gattttttcc
    31921 ccaccctttt tcttctggga tggcttttga cacatactca tccacagtga taactttcat
    31981 ttttatatta tcttgcccgg gaataacagc ttctagaata aagtctttat caatcccatc
    32041 cttagttcca agattaacta ttacaccgtc ttggttaatc ttgattatcc ttccatctaa
    32101 aggaattgaa tttgaaatcc gcttcgcaac acgaacggag atctcagcaa tagaatttcg
    32161 attcgaagca gttgtgttaa ttgtatctat tatttttcca ctgactcgat catagatctt
    32221 atatctaact gttagattat ttttatcttc tgtaaatgtc ccatatgcta tatatcgaat
    32281 ccgacttcct tcttttctat ctatatctaa aatatcaata ctatcaggag agtagtaaac
    32341 agattctgtg taggcttcaa cacctttctt tttcttaatt gcatttctga tctgagcttc
    32401 ttctgatcca gatattaatt ttaaatttgg gtgtagacta attgcaaact taattgcatt
    32461 tgcaatttgt aaactagcat cagggtatgt atataaactt ttatccggtt caggatcaaa
    32521 tataaatatc tcaggctctg ttctaaatcc ctggttcatt ctaccagact cagaagtaaa
    32581 gccctctcga taaatcaaag tttgcttata tttatgatac acattttcga ggcggttgtg
    32641 aattttcata tcatccacgt tatcatctcg catttttgtt aagtaatgga ggagtcgata
    32701 gagatcacca tttctataat agtattctaa taattcctgc tttaactcag aatttgtagg
    32761 gattaacttc tcagctcttt tcaaatgaaa aaatgcttca ttgtataaat attcatttct
    32821 agtcgctcta taacgatcta gtctatattt tccaagcgtt acccgcaagg tatgattttc
    32881 atttagtttt gaaatagcat aattctcagc ggaaaatctt cctagttcat ttagttcttg
    32941 tagttctaaa agtcttaaat agttttctgc tgaatcctct ccatgattca atttataact
    33001 aatatatcct gatatatact gtaaataggg atcatttgga taatcatgta ataaatctaa
    33061 ggttatcttt tttgcttcag catacttctc taaattatcg gttgtatcaa atttaccaag
    33121 ccagagaaga tttttaatta aaaggcgttt actttcatga ttatttccat ctaaagaaag
    33181 agcagtatgc aataatgtat aagtttcttt catcaaacga tatgtgtcag atgaatcttt
    33241 cagcatttca aaatttaata tagctttttg attaaatgag tttggattct ctggatgttt
    33301 cgcaattaaa tcatccacaa tttgatttgc ttttttaaaa tctttatttg ctatataaac
    33361 ttttgcgagt aatagcttaa actcataatt ctgaacgact ctttttttgg cctcttctag
    33421 cttgatcaga gctaatttat ttttccctgc ttttaataaa acataagctc ttgcaagtaa
    33481 caattcagga ttatacggtt cttcctttaa ggatgattca agcaaatcca ttgcttcttt
    33541 gtattttcct aaatccgctt gtatttcggc taaacctgct atcgcttctc tattttttga
    33601 atcgagttcg agtgttctct taaagccggt ttccgcatcc tgcttggatc ctaagtttag
    33661 ggaactcttt gcaaaaccta aatttgctct gattgatcca ggatttttaa tgagtgcttg
    33721 cttatagctt acaagtgctt ctctgtaatt tttttctttt aggaatttct cacctaattg
    33781 aatatactcg ccggcttcct tgtcctgagc ggctaatgat attaacccac ataaacaaag
    33841 taaaatgtaa aataagatat tctttttcat tgtttctctg gcacaaagaa tccagactgt
    33901 gccctcccaa cgatatcccg ataaaaagaa gatatttcaa catcaatata acgtcccgct
    33961 aaatcaggat ctaacttact tttataagaa ataatatatc ttttatcctg cttggactga
    34021 atgaatttgt ataaattcgt ctcttcttgg ctccctggaa ctctcatata cattccacct
    34081 gttctgtttg caatgtcctt ataaacagat gccatctctc cttcatcaga gagagacatt
    34141 ataatgacag gaatagcatg agcatttgca tattgaatat ttctaagaac agtatattga
    34201 ttaaaagcag aaggaagaac gtcacccgaa accaacaaaa taacagctct aggaccaagt
    34261 tcaggaacta gatctcctat tccttggtag agagattttc ctaaattaat gctagaatcg
    34321 gttggctcag attttcgaat cttggcatat atatcgagag gcgagtaacc aaaagagtaa
    34381 attttttccg cgtcctttcc tgctctaatc acttcaattt tgtctttaac agtgattgaa
    34441 ttaaagaagc ttcctagaac cccatctata tttttagaaa attcacttat cttcttactg
    34501 ttttcataca caagagcaac actcatattt tcgttaaaag gtttcatctt ggctagccca
    34561 acgagagggt aaacgttttc attttcagtc actcggaagg ctgcacgacc gattcctgtc
    34621 agatcttgtt tacttctatt tctaactctg acaaataaag aaacatctgg aaaacgattt
    34681 agctctattc tctcaataaa cacatttaag tttgatgtca gtgtattttt tggagcaaat
    34741 acatctactc tatgcctgtc ataatcaacg gaataaagag aaccagttgt atcataagaa
    34801 cttgcaaagg atctaagtag cttaacgtat tttcctgttt catctctaaa gctagcaatc
    34861 ttagtccatt tatcactatc catattgtag atcatgagac cattttgctc atcagctaca
    34921 tacattcttt ttttgaaaaa tttaacagaa cgaggaactg tcatatttgg atgtgtgtat
    34981 tcttttatgt aatttccttc atcatcaaag acagcaattc tcttatttcc cttgtctgca
    35041 acatacacca gattattttc gatggttaaa ccggatggca ttttcaattt acctttacca
    35101 tctttaccga actcagagat atatttaccg tctaacgata gtttttgaac tcgattattt
    35161 ccagaatctg caatatacag tatgtctttc tcggatactg aaatgcctgt aggtccataa
    35221 aatttcccgc cctctgaacc ttttcctgca aaacgttctt gaaaatatcc tttatcattc
    35281 aaaacgtaaa tacgatcaga ggcaaagtca cttacataaa ttaaaccctt gtgataagtg
    35341 aaaaacaaag gtccctcaaa agttcgacca aaactaccct tataatttcc aacagggatt
    35401 ccatttgtat ctagcttgat tatatttgca gtatcaaaag ctaatataaa taaatttccc
    35461 tcttcatcaa aaagtatatc tgtcggattg cgaaaacgat agccgcgatt ctcatcgcca
    35521 gataaatgct tataaaaagt tagattatca tttttgccta cacctgcaat atgtaaacgt
    35581 aggatttctg ctctgttctt ccagatttga ttcatctttc ctgatgcttc aatgatctcg
    35641 agttcattta aactttcctg ccattcccca cttaaataat tagcatcgga aaggtatttg
    35701 cgagcgagct taaagtctcc catgatagaa agagcggcta taaaactttc tctcgctgca
    35761 ttgtattggt aattgtgtaa atatactaag ccttgcttga aataggttct agcttgttct
    35821 tttcctaaac gaaattcagg cagctcctct gttggggcta acggctgtaa tactaataaa
    35881 atcagtaaag gtgaaaactt taaaaactga cgagtaatga ttcctacttt atgacataat
    35941 ttaaacatga aactttataa gcccctgttg ggttaagatt aggcgaaaaa gtttcattcg
    36001 cctttattta ttatcgagta aagtctttaa atcgttagtt aagtttgcaa gtgtatttgt
    36061 ataacgaaga gacttttctg ttccttcgga agccattttt gcaagcttgt aaacattcaa
    36121 aatattatcc attacactgt tagaagattt aacaacattt gatgagtttt ttgcgatctc
    36181 atttagggaa tttacctgct cttctaagga tgaatctata tcgttttgat tgttcttgat
    36241 agataacaat tggcttgaaa tttcattcat agattccaca gctcttttgg agtcttggta
    36301 gaaattttct attttatagc gaatatcatc tgctatctta tttgtttctt ttgctaattc
    36361 tttgatttcg tttgctacaa ctgcaaatcc tcttcctgcc tctcccgctc ttgcagcttc
    36421 aatacttgca ttgagtgcaa ataaattgat ttgttgtgca agaccagcaa caattttatc
    36481 aattttatcc aactccttgc tactttctaa gaggtttgaa aatacattgt ttgttgtgtc
    36541 agcagtcttt agggctgtga tagaagactt ggtagaatta gaagaattcg aagagatctt
    36601 cttgattcca gtctttaatt tttctagaga actacccatt gcaattacat tgtgactgag
    36661 ttgcttagaa gcattcgaag cttggctagt ttgtttttcg agcttgctag aatttttatt
    36721 taaatcatct gccatctcag aaaactcatg aatcgtctct gatagggagt aaactacatt
    36781 agaaagttcc gaatcactag caccagaagg cgctccttct tttacaggag gtttaataga
    36841 cttaatggtt gcaaatggat tatttccgac tgtttttcta tcattcatat tgagttccta
    36901 ctttttattt tcctgagcta taacttctgc tgcaaatgat ctataatcat gtgcacctat
    36961 acttttaggg tcatattgaa atatcgtctg agtgaagctt ggagattcag cgatttttat
    37021 atcctcacga atataggtgt tatagaccaa tactccaaac ttttcaatta gaatatctac
    37081 tatttccata ttgtgtttaa tattttgatc cactcgacat ggaagaatcc ctgtaatcgc
    37141 taaactcgga tttagttttg cttgaacaga ctctaatgtc tctaagaggg aaagaactcc
    37201 ataaagagca agagattggg caaccacagg gatgatgatt tcgtttgcag cacccattgc
    37261 gttgagtgtt gttagattta aacctggtga gcaatcaaag attacataat cccagcgttt
    37321 tggatcgata cctgcaaatt tatttttcaa gatggatgct ttattaggaa gttccttcat
    37381 ctcttttgaa tatttattct tgagaggaac gaatggaatc atttctaaat tccgaatctt
    37441 tgttttctga gcaagtgcaa gaattacctc tggtcctgca tgcaaatcaa agagatcttt
    37501 atcttgaacc gaactgctga gtcctagcca aatagaagca gaggcttgtg gatctagatc
    37561 gattaatagc acacgcttac ctttttctgc aagagcagca gataaattta cagcggttgt
    37621 agtctttcca ctcccgccct tctgattgtt aatgacaatt tttctcataa ttcatttacc
    37681 aaaaatttaa gaaattctat ccgacctaaa ttctaccacc ttttcagagt ccaaaatcaa
    37741 taggagtgtc tcatcgatac gatacgcttg cgaaacaaaa taccgatcca ctccgtgaat
    37801 tactttagga acttcaatcc taatatttcg agtaatttct tcaatatctt ttagttcatc
    37861 taccagaaaa cttacaagac catatttcgt gcgcacaatt acatgtgcat aattttcgcc
    37921 taattcaatc tctggtaagt cgaaaacttt gcgtatactg atgactgtaa taatttgtcc
    37981 cctgtattga atcaagcctt tcacaaaacg acttgccata ggaacccgta tcatcggtat
    38041 atcttttaca agagcgaaaa cctttccaat ctcaacaccg aagaagtttc catcaattaa
    38101 gaatgtgcag aactttttga ccgtcattcc atcacttcct ctactacttt cagatctagg
    38161 tgttttagat gcactctcat gatttcccct acatctaaaa ttaaagctgg tgtctcatct
    38221 tttaatacag taagacctga gaataaatac acatttttaa tttcaaaatt caaaggtctc
    38281 accacaactt cattcatatc ttcaacggaa tctgctacta atccgtattt gaattggtta
    38341 acttcgacaa ttaaaatgga gatttttttt cctgcgtctc tttgtctatc ggcaacttct
    38401 ttatcatctt catacttaag aatttgagaa agaaataaaa ttgggattaa atctcctcta
    38461 tgcttatacg tagggaaacc gttgatcact tctaatgtat taataaagga atcactctct
    38521 gtttgcataa tctcatatag atttgcgcga gtgacaatgt atctttcctt tccaacagaa
    38581 acgataaccg atggaactat ctcatcgatc aaagggagag tgatatgaaa ctcaactcct
    38641 ttacctggaa tatttctgta tatgtaaaac ttaccagcaa tctgttcaat attagatcga
    38701 acaatatcca aaccagcaga tgatttagaa ctagagaatc cctgttgaaa gataaagtct
    38761 attgcctctt catcattgag cctttctgct tcttctgtta atagaatatt tttttcgatc
    38821 agctttctct taacgcgagc aggttcaata ccagaaccat catctttgat tttaatataa
    38881 aacaattcac catggtgaaa cgcattcaaa tgaatttcac ccatagctga tttgccaagt
    38941 tctgttcgaa tctctggcat ttcaattcca tgctcaacgg aattttttat gatatgaatt
    39001 aaacaatttc gtaatacatc gagcatacga gtatccaatt ccttttctcc accttcaatc
    39061 aaaaaggaaa cctgcttgtc ttgcatttga gcaatgtcag atactacttt atcaaaatta
    39121 tgtaaaagtg ttcctaacgt ttttgttttg gctccgtgca cttttgtata taagtctcga
    39181 actaaaaatt ctagttttcg tgcatgtgtt tcaagaggag aatttttatc attagaagca
    39241 agctctttga tttgaacctg tgtttgtaga agtggaataa tggtattcat taaagaatcc
    39301 attgtttcat tttctgtaaa gatcggtgtg agtgcgattg gaatatctgt agaggataca
    39361 cgagaagagt cttcatcctc atcatcactc gacgcaatga taaattcctc gccggattcc
    39421 tgtttattga tgagctcttc tatatcactc accatactgg aattgataat ttgaggttcc
    39481 tttcctgttt catcaatcga aaagataata tcgcgaatac tataatttaa ttttaaaata
    39541 atcgttataa tttcatttgt aaggtcatat tcagaagatt taagtcgatc tactagcttt
    39601 tcaccaatta aaaccataga agctaacttg ttaaatccga atagcttagc cgaatctaag
    39661 aaagtcttat aattggcatg aatggtttct aaatgttctc gttgatctag attttttcca
    39721 agctgaatca attgcttatc taaagaattg agagtttcat aactttcatc tagaaataac
    39781 tttatttgat ctttttccaa acttttgtaa aggctccctt tatctatttc ggcataggtt
    39841 tatcgaatat tctcataaat acaatccatt attttaagaa agcagttttt tttaacatta
    39901 ggcattttta cttattttca taactctttc agtcaatata catacacaaa caattcgata
    39961 ctatttttac ataccactac ttctcataaa aaatttctgc tatttttcac aaaaatgcta
    40021 ttcccaattt ccaaaacagc gtatttaatt tcaaatcaga agttactttt ggtaattggt
    40081 atcattctgt ctttttacaa gacataatca atatcaagtg tgaagttcct attgacgata
    40141 ccccaaatct cccttaagga tagatatgga gatatagtta ggagaagttt aaccaatgtt
    40201 tacccctaag tttaagaaaa gtttgttcat atgaaaacga aaagataagg tatcggtttt
    40261 ggcttgtgtg atggtctgtt gatttttcat tttcaattgt gaatgacttg gcatcgaagg
    40321 aatctttact caaataacgc aagttcgata taacaaaaat ttaacagatt acctccaccg
    40381 attacgaaca ccgatcgcgc ttctaacacc gataacagaa cgataaaaac gattgcttcc
    40441 tattgcggta aagctccctt aaagatagga ctaaatttcc tttaaagata ggtctagaga
    40501 tatatttagt ggaagtttaa ccattgtttg cccctaagtt taaggaaagt ttggtgataa
    40561 gtaaagcaaa agacttggaa tctatgtcac ctatcggtgc aaattcctag aaagaaaagt
    40621 ttaagcctct ttgtaaattt tttgtttgat gatatctact agcgtttaca tcgaacatgt
    40681 ttaatatctt ttttatttag gtaatctaat tcgcctaatt ctacaaggaa ataataggct
    40741 ctagcctctg aataatgatt agccgtccag taacctgaat ttttagtttg ttgccaatcg
    40801 gatttccatg attttgttat acctttttta tatgcaatct ccaactcatc aatgctaggt
    40861 aatctcattc cataaccagc taatccgaga ctaccgcatt ttcttttagc atcttcccaa
    40921 tttagttctc cttgataatc gctccaatgg tttgatgact ctatattttc atcatttgat
    40981 agtgttgcac agctaataaa atggaaaaat aagattagga atattgatac agttagcttc
    41041 attcttttat ttctcatgat ttattttata tttttttcta aacctgtttc aaaaaaaatg
    41101 aattcttttt ttacttccat gtaatcttta ccaacatcat cttttccatc gaacgcaaac
    41161 ttagtattag gcggtagttc ctttagagta atagtgaagc cggggacagt attcattcta
    41221 tcaaatgcgt ttgcccatgc tgtgtatgat ttgcgtgaaa tggctgaatt tggatttgtt
    41281 ttaatttcaa tttgataaat ttgcccgggc ttaaaattaa cttctgttcg taaacttctg
    41341 tgaataaagt tatcgaattc attttcagga atcatttgac tggagccgcg cagtaacctc
    41401 aaaagttcat agcgtgcaat atcttgtgca taattaaaat aaagaattat agaaaggatt
    41461 cccgctcctg atggaacagg aaataaatac tctgatccta aagccataaa agtgcaattc
    41521 cttcttttaa ttgtaggatt ttcagagatg attcgctctt ttattttaga gcttacttga
    41581 taattcatga aatcatcttt tcgaggattt aaaatgttat ccccaaattc gattagaata
    41641 ggcagagatt tttccgttct tctataggat tctaagtttt ggtctgactc ttcgtttcca
    41701 tattccaaat actcattgct taattcagga attttcaaat aaagaaacga atgcttatgc
    41761 tttaagcgtt ctacaattgt attacttttt tgattgcaag gctttactct atgttcggtt
    41821 tgaacttgac gtctatacca ctctagatat tgagaactac ttcctccaaa agaatttaaa
    41881 ttagtaaaac aaaagaggaa gagtaataat ataaaagatt tctttctagg aaatttcatt
    41941 tcatttatac ctttcaattc ggaaattcaa actaaatact atttatacta ttacctatcg
    42001 tgtaaaggat tatataagga agattttttt aaatcagtcc attagtcgta aagaatccga
    42061 tacaatacat gtttacaaag tgggtgatct ttttccaagt taggatgctc aaaatccatt
    42121 actttttgca ttccaatttt aatcataaca ttttcagatt tagtatttaa tatggaggta
    42181 aaagaataaa tttcatttag attaaattct tcaaagccct tttgtaaaca aagctttgcg
    42241 ccttcggttg cgtagcctct cccccaaaaa cgagaatcta gtctccatcc aatttctaca
    42301 caaggagtaa aggaactttg aaaatcagca taatataatc caatgaatcc aataaaattt
    42361 tcagaatctt tttcttctac agcccaaaga ccaaaattcc atttttcaaa atgagattga
    42421 atgcgattta ttaaattgat tgattcttct cttgaaagaa gttgcggaaa ataccgcata
    42481 acctctaaat cagaattcat attcgtaaaa cgggatacat ctgattctct ccactttcta
    42541 aaaccatgtc tttctgattc gaagagatat ttcataatta gaaaatatcc aaaactttgt
    42601 attctaatcg tattttttct ttctgtcttg ctcgcagtct ctacagatat tttggataga
    42661 aggtggacta tagagaacga taaaaatttt cccccatcta attcaaaaca tctctttcta
    42721 aaacactcgc aatccttttt tgctttaagc cattcacttg taagatagaa atgggagaca
    42781 attatgggag aagttcgatg caaattaggc gttagccgct agagagataa ttaggcggcg
    42841 gataacttca acaaatgcag tgcctttaac ttatcaaccg gttttcgaac tgttaaatca
    42901 tagttcatct gtaaattcat ccatagttca ggggttgtcc cgaaagattt tgcaaacaac
    42961 catgcagtct ctggagttat gtttcttttt cctttgataa tttcatttat agtttgcaga
    43021 ctcaaaccta catgccttgc aaactgagtc tgcgtaatat tagatggctc taaatactct
    43081 ttccataata taattccagg atgtgtggat tttcgatttt taggtagcat aataaattcc
    43141 ttttttatta atgataatct tctattttca cattctcaaa tttattgctc aaaacctgaa
    43201 aagtgattct gtattgatcg ttgattcgaa tgctgtagac attcttcaaa tcacctttta
    43261 attttttcaa tcgattgccc ggcggaaatc gtaaatcttc tacattcaaa gccgcattga
    43321 gcatatcaag ttttcgcaaa gcaatttcaa caagctcttt cggaaattta tgagtcttac
    43381 tcgaagtatt accatgaaat aaatcttctg taaacttatc cccgaaattt gctatcataa
    43441 attcatttta aattatacag gtattcaagt aaagcatgaa aatatggcaa agctgaagta
    43501 ttttgtttga aaatcttact ccgtttagtt caaaacaact tattatgaat gactcgcaat
    43561 ccttttttac tttaggtcat tttcacgtgt aagatagaac gggaggtgat gatgaaaata
    43621 attgaatacg aattatacgt tgtccgctat atttataaat attttttaac atatactaaa
    43681 ttcagaacgt agaggaaatt taaaattact cacatagacg cataaaattt caccttcttc
    43741 catactcggc tgaatccttt gtctaaagtt gccaattttt gtcatcagtt cttctagaga
    43801 tacgaactga gaagctacaa ctctatctaa atcctttttc ttttaatcga cttttctgac
    43861 tctaattact actaggttca gactagtgcg tatcccgtct tgcctccaaa tcaaagtaga
    43921 tctaatccaa gaaagcctac atttccttta aaaataaaga gataccagac ttcaaaagaa
    43981 acttccccaa ggggatttta ttgaaatagt gcctggtgta aatattctag aattctttat
    44041 tgtctttatt tttcaaaaat tctaattgca attaaaccac cgtcttattt catgtaattt
    44101 aatacatgaa tttattttca catattaacg gagaaacaaa atgttagctt ttatcactct
    44161 tgtcccgcta atcacacttt acgggatact ttatctgact ggttctaatc tgatttctat
    44221 ctcatttttt gggaacagat tggatttggt ttatttcgat caactcagtc aaatcatgtt
    44281 aatctacatt tctattcttt ctttttttat tatttcttat gctaaaaaga attttgaaag
    44341 agaacgaaac ggaagcaagt ggctcttttg gttttttatt tctctactca gtgttcaatt
    44401 tcttgttatg aatggggatc tattattgac cgttttagca tttattagca ttagtttaag
    44461 ttttcacaat ttactccttg cctttccaca aagacaaaaa tctaaactag ccgcgacaaa
    44521 aaaatttatt ttatccagat tgggagatct aagtttccta ctcggtgcat tcttttttta
    44581 caaatccttt ggcactttca atattcaaga cgtcatttct catttaaatg atttagatct
    44641 acagacaaga caagatctta tatttctccc ttctatcttc atcgcttttg cttgtttaat
    44701 caaaagtgct cagtttccat ttcatttctg gctaccagaa acgatagact ctcctactcc
    44761 tgtctcagct ctcatgcatg caggcatcat caacgcaggc ggttttatct tagttcgact
    44821 tcgattttta tatgaacctt ttaatttagc atccatactc attcttggat tggggttaat
    44881 cactattttc ctcggaggga tcagcatgct tgcccaatct gatgtaaaaa gaaaattagc
    44941 tttttctact atcggtcaaa tgggatttat gatggttgaa tttggtctag gtctttaccc
    45001 gatggtcatt ctacatttga tgggacatgg gttttataaa gcttatgggt tcttagcttc
    45061 aggctcaaga gatattgttc caagtcctaa actacactct aaactgtatc gaaccctcct
    45121 tgctggtttt gtaattattg ttccacaagt tgtattcatt gcaaccaacc atttagagtt
    45181 gacgcttgtt tctatattca tcatcacact tattggcttt ttaccattaa agctaatcaa
    45241 gagaggaatt ttaattacaa ttacattagt tctttttgta atccttattc ccgaatatgc
    45301 tatgaaaata actgcaatcc cctactcgac aaatcaagtt ccgtatatga tatctttatc
    45361 cgttattctt tgtctctcta ttctttctgg ttttactttg agtctaccgt tccttagcga
    45421 aaactctttt gtgcaaaaac tctactgcta tgcacagcga ggatttttgt ccaattactt
    45481 atctgataaa ttcatttata aactttttta aggagaatct tatgaacgca ttatctacaa
    45541 atccaaacaa ccaaaaactc aatacagata gcatttatca attgttaaaa aaagttcctc
    45601 ccctttggga cttagattct tacattgctg taaatccttt cctaggttat tccaatcaac
    45661 cctttgttaa aaccatggaa aaattggaat ccattttatc tatgaactta attcccgaaa
    45721 aggacttgtt agatgggaaa gagatcaaac atgaaaatgt aggagagttt gatatgaact
    45781 atgccaatct ttcacaattg attggtcctt ttctagcaag ctatatagat gactcgatct
    45841 cttattggaa aaatccttgg aaagaaaaaa gtttatggca agcatttcag tcttggtcag
    45901 caatcgatcc agtttggaac ctaacgtttg caaattttcc agtcctcaaa gatctacccg
    45961 atgctgctat tcaagctatt caaaaattag gggaggaaaa ttcttccatc caagttgaaa
    46021 gtttagaaaa attacttttt attctccctg gttgggcttc ttatttcaga aaaaacaatt
    46081 ggtttaaaga actagaacca aatagcgatt tagtaggatt acttgcaatt ctagcctaca
    46141 tctacaccta tcgtcccgaa ttgttcactg ttaattttaa gctaaaacat ttagataact
    46201 cagaaaaatt tcaaaatcgt tttgattatc taaagaaaaa agaaatcgaa tacagaacaa
    46261 aacttcttca agatttaaag aaaaattatc caatcccaga agaaaagaaa agtgatactc
    46321 tacccaaaac aaaattccta ttttgtattg atgttcgttc tgaatcaatg agacgacacc
    46381 tagaatctac ttccccttct attgaaacag aaggattcgc tggttttttt ggaatgccaa
    46441 ttggttggaa acattggaca gaggaagaat tcagtcacag cccagctctt atttctccag
    46501 cctatgtttt ttgcagcaat gaaaaatctt tctctttacg aaaaaacaaa ttacaaacaa
    46561 agtcttggat tcaaaaaata aaacgaacct ttccaattgg atttcaatat gttgagacag
    46621 caggattttt atcattcttc ggattaattt acaaaactct gaaaattgga ccgttacacg
    46681 aaaatccaag agaaatttct tccgaagaaa tcaaatttgc tctctcatct ttgccgcaaa
    46741 cagataaaac aaatattgcc gcaggaattt taaatcactt aggtttgaaa gataattttc
    46801 cctcttactt tatcatcaca ggccatggta gtctaacagt gaataacccc catcaagctg
    46861 gacttgcttg tggtgcctgc tctggacaat caggagaatt aagtgcaatc tttgcgtccc
    46921 aacttctgaa tgatttggaa acaagaaaac aattaatgca aatgggtatt tctttaccag
    46981 cagaaacaaa atttatccca gcgatgcatg aaactgtaac ggatgaaatt attttcttag
    47041 aaaaagaaaa tatagataaa tcctatctac aaaaaatttc tgaattaatc aaaaaagcta
    47101 gtttagaaaa tcgaaaggaa aaagcacgta tcatgggttt aaattcggaa agtccttacg
    47161 ttcgatcaaa agattgggca gaagtttttc cagaagctgg acttgctggc tgtgcgtctt
    47221 tcattatcgc aaatcgttct agatcaaaaa atgcaaattt agaaggaaga agttttttaa
    47281 attcttacaa ttggaaaaag gattctgaat ttaaaacttt agagctactt atgactgctc
    47341 ccgcagttgt tgcttcttgg atcaacttgc aatactatgc atctactaca aacccagatg
    47401 tatatggagc aggaaacaag ttgttgcata acgttaccgg aaacaaagga gtttttgaag
    47461 ggaatcattg gaatttaaaa tcaggcttac ctctacaatc tgtatttgat gggaaaagat
    47521 ttgtgcatat accaattcgt ttacaaatca tcatcgaagc cccacaagaa gaaatagata
    47581 agattttaaa caagcattct tctattggtg atttagttaa aaacgaatgg gtttatttaa
    47641 tccaatggga tgagaatgga gatttttatc tcaaaaatca aacatgggaa aaaataaaaa
    47701 tctaactcac tttcttagat gagattatat accaaacgcc caaagaatct tccacaaaga
    47761 ttttattgga ataacgtttg gtatatatcg atttgagtct tgccaaaatt aaatcataaa
    47821 ttcagaatag aacgcaatgc atgctcttat tgaaaaatta aaacgattgc agattttcga
    47881 aaacacctat cgattcggaa ttgatagtat aaactgtgaa aaactacttc gttctattct
    47941 aaatttccca tgtatttgtt tagaaagcat ctgaactaat tgcaaaccaa atcctttttt
    48001 agattcttgc tcgctcgaga taccgacccc atcgtcttct accttaaaaa taatctgatc
    48061 tttttcttgg taagcggata cggttaataa accgctttct cttccgataa acgcatactt
    48121 catagcattt gtaattaact catttaagat aattccaaca gaaaataaag ttgaagatgg
    48181 taatattaaa ttttcaatat tcttatttac tttcacaatt cggtaatttg ggaaattata
    48241 tacaatttca tcaatcagtg ggattagata ttcaaataag gaaagatctt gataattaga
    48301 agacatatat aatttatcat aaagtaatac catactttgt attcgactaa ttgcatcgtt
    48361 taacacttct atggctactt tatcctgcaa actatctgtc tgtaggtaga gaagacttgt
    48421 gattgttagc atattgtttt ttatacgatg gtgaacttct cttaataaaa gatctttctc
    48481 tcccaaaaga gtttttattt tctcctctgc agtttttcga aacgttatat caatagcgaa
    48541 gccactccat acaacaggct gatccggatc tttttttgga ttgggtcttg atgtgacctg
    48601 cacccatttt tctgatccag aaggcataat aaatcttacc tcagttgaaa aatgatcttt
    48661 agaatgaaaa gcttcatcgc tatctttttt aaattgctct atatcatctg gatgaactaa
    48721 attccaaaga atcgtcatat cacgcattac atctaccgga tctaattcaa atatttctaa
    48781 gcatttagga cttacataca gcatttggct tgttccatca ggatgcaaaa cataatcata
    48841 aagagcaccg ggaactgttt ccgttaggcg atgatatctt tctatattct cctgaatcgt
    48901 tttttctgcc tcttttttat ctgttatatc ttctgatata ccaagcaaat attcaggctc
    48961 tcctctatca ttaaacaaag gtaatttttt tgtgtgtaag attctagttc catttggagt
    49021 gtttataaac tcctcgggaa tatcaactag ttgaccgtta tgaatcacat ccaaatcttt
    49081 ttttgtaaaa aaatctgctt gctctttagg gaaaaagtca taatcatttt ttccaattaa
    49141 ttctttacga ttaaatccta ccaattcttc tcctgcgcga ttaaataaaa taaatcgcaa
    49201 atcctttgca tccttcataa atatcatatt tggaatattc tcaataatcg aattcaggaa
    49261 atttgtagtg tcttttattt ttttatcaaa tttcttcttt tcagatatat tcctataaat
    49321 gagtagaatt ccattgggga aaggatgaca ttctataaat attgtcattt gataggattc
    49381 atagattgca ctgaattccc ttttcttttg agtgcgcatt gcttcttttg gctcttctaa
    49441 gaaaggtgtt cccatcactt ctgggaataa ctcccagtaa acctttccaa taacgtcttc
    49501 ttcttttaag ccaaaatcat ttaatgtttt cttgttaacg taggtatatc tccaatccaa
    49561 attgaatgca acgatgctat ctgatattaa atccaataaa gaattgatat cattattaag
    49621 taaaatcgat aaaggctcaa tacttatctg ctcagataat cttttttcag aatcatataa
    49681 ttcaagagcc aattcaattg aattttgcag acaataatca tttgagtttt ttgagtgaaa
    49741 tccatagcga ctaatgcttt ttaattcagc caacttgctt ggttcaatat gtgaaaacat
    49801 aaaaacaatt ggtatattat ctcttaatga tatttctcta gaaatcgtta gaccaaaatc
    49861 aactgcaaaa agaattaaat caggcttctc tacttgaaaa gcctctacca attctatttc
    49921 acttgtagca atacttacat tgtaattcca gttttgaatt tttttagaaa gcgcatttgc
    49981 aaaaattatt tctttatcga ctattagaat tcttttcatt ttactagagg tatcactcat
    50041 ttcattcctc cttatatttc attacaaaca gatatccttc taatatcttt ctaaaaaaac
    50101 tgagatataa aaaggagata gcaattaagg ctgaaactct tgatcgagaa taatccaatc
    50161 atcactttga cgattgtgcc attctttctc aggatagtat ttgccgccaa tagattcgat
    50221 gattcttttc atataaggtt cgcctggatc aaaatccttc ccatagagtg caatggaacc
    50281 accatatttt ttctttgctt gcgtatgaga gtagattcct ttcaaatctt ctattttata
    50341 gttggtcaat ggaatattat atttatccgt tagttccttt actagtctac tgactttatc
    50401 tgcctggcga ttatttgcca ttaattcatc catattcttt ccgacaattt cgatctgaat
    50461 acagttttga tttgttcctg tcgctgcttg tgccatatcg tctaaataat ctagaatctg
    50521 ataagcctta gcgtccttat ctaccatgat tgtagcagca agctttcttc tctctagagt
    50581 ctcttgagaa acaacgaagc tagatattgc tgtaaaatgt agaactacac attctggttt
    50641 tattttttgt ttgaaaacat acttcaaacg atagttctca ggcgtaaagc cttcctcgtt
    50701 cttctctaat tctactaatt caacttgggt tggtttagta attccccttc ctctatcaaa
    50761 gtctggttct ggaatctctt tgtttttctt ttctcttcta gaaacccaat ctctttcata
    50821 tctcccaaac caattctcct ctgcataata ttgtcctcca aatgaagaaa atatttcttt
    50881 taaaatcttt tcactcccac attcatttaa atctacaaaa tttccaaatt tcttttttgc
    50941 ctgcgtgtga gtgaaaactc cggagagaga atttatattt tgattggaaa atgggatttc
    51001 taaatcattg atcaattttt taatgagttt tccggcagca tttaattgag ttttattatt
    51061 taagatttcg tcttctttac cttctaaaga tatatgaaga ctaacgtcat ccattctagg
    51121 cacagccttg actgcaaggt atgggattgt attccatcca tagtattttc catccttacc
    51181 tacaataaaa tgaactaaaa atccagaatc gagtgaatct tttatgtatt gttcttttga
    51241 tttggagtca gtatgatgta aaatcagaga gctaatgtat gtatttcgct taactccaat
    51301 tatttttgaa tgcaattcgc tacttaacca atcttgataa ggaattatct ctaaatcaac
    51361 ttgataggaa ttggatcgtg tgattctggt atcacacacg caggaaagta aaatactaag
    51421 aataaaaata gaaattctta ctttcaagcg cgctgaacca tttaaaggta ttagatacct
    51481 ttcatttgtg cgagttgtaa aatagattta acaatttcct cttgagagag atattcacag
    51541 ttgtaactaa cgtcaaatac atttaatcca ggactttcac ccgcaaaata attacgtaag
    51601 aatatacgtt cattatctac tacattgatt ctaccgattg cttccgcatc cgattttaaa
    51661 ttctctaaat ttttaacgcg tccaattctc caagaaacag aagcatgtaa atgaacatgc
    51721 acagcctttt gtaaatcacg agcaagaaca gcaccgcctc gaccaaggat tacaacattc
    51781 ccatgaagtg caacagttct taaaatacgg gcgattgtct ttttaatttt taaatcactt
    51841 ggaagatgaa tatctgagaa agctaaaaat aattcttcaa aaacaccttt tggtttggac
    51901 ttagcaattt gatcgattag tttagccgga atatctaatt cctctgctgc ctgattgacg
    51961 atctgcttgt caacaacaga ccattcggct ttgtttgttg ctgtcaatgc ttttgaaact
    52021 tcgttagcaa ttggaatccc agggcaacca tattcccttg aaattgtaat tacacgtgga
    52081 acactgagtt cactgtttac actgtcagaa ttaatttttt ctttgatata gtctaaaatt
    52141 gaatttcttg ccatttaata cctttcctaa aaaacagttc atctatcacc taatggattt
    52201 ctagtttaca tggcaattac ttttaatata atctagattg acttatctca taaataccta
    52261 catatttata tgaatctaga gagtagaagg atttagaatg gtttcaaaag aacaatcatt
    52321 aaaggaacta agaacgattc caggtgtagg caaagtagtc gctaacgatc tctggaattt
    52381 gggaattaag tcaatatccg acttgatcaa taagaaacct gagaatctct accacaaaca
    52441 caatcttttg aagggaaaag tgcaggatag gtgtatgctc tacacattta ggtgtgccgt
    52501 ttattttgcc aatactgaaa aggacttcca agaaccagaa aaattaaaat ggtggaactg
    52561 gatggatgat gataaaaaaa ctcaccacaa aaaagcagga aatcaatgaa agaactaaca
    52621 ccagatagtt atataaataa tttatccaaa gagagaaaag aggttattac aaaattaaga
    52681 gaccttttaa aaaaaaatct accaaaaggt tttgaagaaa caatcagcta tggaatgatt
    52741 tcgtatgtag ttccccattc acttcaccca gaaggatatc atacagatcc aaaacaaccg
    52801 ttgccattta ttagtttagc atcccagaag aattttatag ctctttatca tatgggaatc
    52861 tatgcagatg aaaaaatatt acattggttt gttactgagt atcctaaaca ttcaaaaaca
    52921 aaactagata tgggaaaaag ttgtatccgt ttcaaaaaaa ttgaccaaat tccctttgat
    52981 ctaatccaag aactagttaa aaaaatgact cctaaacaat ggattgattt atataaaaaa
    53041 agcattaaaa aatagtttca acctatataa aaacttctta tttaagaatg atttctaatt
    53101 gagtaaaaaa aattttaaca atacaaacga gtttaagaat cattacgcct taaaggcaaa
    53161 tcattattta ccgcttcttc ttcggaatcg ttttaaccat tgaccgatta tttttaggtt
    53221 atattctggt atcagaaaca aagaaacctg ttggaagttc aggtatatgc ttaaaacaat
    53281 actaaaaaga atttttaaag ataaggctcg tttttatttt tttaccattg tatttttctg
    53341 gactggatca gtcaccctat ccctactttg gaatattttt agcgtgaaaa caagtatgga
    53401 aagtattgcc tatgtaacag caaagtccag ttttgaaaag gatatcaact atcgttactg
    53461 gaatgcaaaa catggaggag tctatgtaag aattaatagc gatactcacc caaatcccta
    53521 tttaaaaata cctgaaagag atttaactac tattgatggt atcaagttaa cgcttatgaa
    53581 tccctctttc atggttagag aagtatttga tatctcaaaa caaacttcag aaatacaatc
    53641 tcatataacc attttaaaac caattaatcc aaataatatt gcagatgagt gggaacaaaa
    53701 agctctgcgt gaatttgaaa ccggggtaaa agaaataaaa ggtctctctg aagataaaag
    53761 tcattttaga ttaatgaaac ctctttatgt ggaaacatct tgcatgaaat gtcaccgaga
    53821 ccaaggatac caagttggaa atgtaagagg tggaattagt atttcagttc ctatgcagcc
    53881 ctacaaagca gaggcaaatc tgcaatatta ttctctcagc attggacatt ttttattgtg
    53941 gattctagga ttcataggga gcaaaatagg atttaaccgt tatacacaat atcaattttc
    54001 aagaaaggat atcgaactcc aaaagaaaga attagattct ttacaactca taatgaatgg
    54061 agtagcagaa ccgatagtag tcattggaat ggattataaa ataaaaatta gtaatcctgc
    54121 atttcgggac ttctttggtg ctacggagga aatgactcgc caattatatt gttacaatgt
    54181 tctttataat aggtctgaag cctgcaactc aacacccgac aatccatgtc ctcttctgca
    54241 taaatatgat aagaatggaa aattatggag tattcgtaaa catactagaa atgattcaga
    54301 agttagaacg actgaactac ttgcgacgcc ctattataat acagaaggta atttaaaagg
    54361 aacaatacaa gtttttagag atgttacaga aagagaaaat gcggaggcaa aattaaaaca
    54421 cttagcacat tttgatacac tcacccaact acccaataga ctcctttttg aagaattact
    54481 tgaagacaga cttcttacag cttcaataaa aaatgaacaa gtagctttgt tttttgtcga
    54541 tttggataga tttaaaaata tcaacgatac actcggccac cctgtgggag ataaacttct
    54601 tgttaaaata gcaaatagat tgaggcaaat gattattagc cctaatattg tagctagatt
    54661 tggaggagat gagtttacta ttttcttatc tacaaaaaat aatccacaag caattaaaga
    54721 catggcagaa ttaataaaaa aagaaattgc gattcccatt attttggata cctatgaatt
    54781 ctttgtgagt gctagcatag gcgtagccat atttccagag gatggtagaa ccttaatcaa
    54841 attgatgaaa agtgctgata cagccctgta tagatctaaa agcaaaggta gaaatctgat
    54901 tcaattttac gatcaaagtt cagaaacaaa ttctcgaaaa agactgcaaa ttgaaagtga
    54961 attatacagt gcaatagaag ggagtgattt attaactgtt tatcaaccac aagtatctgc
    55021 caggactgga aaaataattg gtttcgaagc actttgtaga tggcaacatg cagaattagg
    55081 acttatttca cctaacgatt ttattgatgt ggcagaggaa actgggctca tcgtgcctct
    55141 tggtaagaaa gtattactga ctgcagccaa gcagtgcaaa atctggcaaa tgaattatgg
    55201 attaccttta cgaattgcag tcaacatatc gcctaggcag tttgtaagac aggatctaat
    55261 aaaagattta ttagaagctc tggaaatatc tggactcaat cctaaagact tagaactaga
    55321 aattactgaa acatcaatca tacatgacct tgatacaaca atacataccc tagaaaaaat
    55381 attagagctt ggagttatgg ttgcgataga cgattttgga accggttatt caaatctaca
    55441 atcgatcaaa tccctaccaa ttcactcact caaaatagat aaaagctttg tcaatggagt
    55501 ccctgagaaa gattcagatc gggctgttat aaaagcaatt ttagcactcg caaaaaattt
    55561 aagtttaaaa gtagttgcgg aaggcgttga atcaaaagaa caaagcgagt ttcttgctag
    55621 cgagggttgc gatatcttac aaggatttta ttttggaaaa ccagaaacag caaaagttgt
    55681 agaaacaaaa tttctgtcct ctaatcattt aacataatta tttagaataa tttatcttat
    55741 agagaaaaaa gttttcatct gcaataaatt catttctatc ttttgtttga tataataaat
    55801 ttaaatgttc atcttgaatt ggatttgaag agataaaata aatattattt tgatttctaa
    55861 atttctcaat tgtgtgatct aaatgagaat acgatggacg aatttcccca tcaaccatta
    55921 ctttattcca aagcatatga gatccaaatg attttattac tggaaaataa aattgttttt
    55981 ggttttgaag atagaataag attgttttgg ctttgtctgc attttctaca gaaaaaatat
    56041 tttcgacttt atctaaatca ttttgtttta tgaaactagc catttctttc gcattggaaa
    56101 aggaataacg aatatctttt ctagtttcct tccaaccaaa aggaattgac tgactcaaac
    56161 ttatcacact aaaaaaaagg aggatatttc ttagattaaa atatttattt gtagaactaa
    56221 aggaagtctc ttttttctca gaagcataaa tccagagtgt gactaaaaag gtaagaaata
    56281 cgaaaccacc atgcctatat ccatttataa aaatgaatgt gtaaaatgac caaagcaaag
    56341 aaagagaata taaataaaaa aagaataact ttttattagt aaaaactagg attccgagag
    56401 ttataagcaa aataaatcct gtcaatattc taaaatctaa tttagaaatt cctggagtga
    56461 atgaatataa aattgaaata acagaaccta aattattaaa aaccttaaac tccctcatgg
    56521 cacttccctg agtttcacta cttggaaaga cctgcaaaat taatagaatt ccacctaatc
    56581 ccattatagc gatagacgat atatttcgaa ttgtaaaatc tcttttttct aatacttccc
    56641 atataaacat aagagcaaag ccagatgcaa gaattgagca atataaattt gtatttgcta
    56701 ggagtagtat taaaaatgca tatataaatg gcttttcaaa tcgtttttca aaaaaagcac
    56761 aaatagaaaa taaaaataat atacctatcg cataattgcg tgcaatgact gaatattcga
    56821 aaataaaaaa atagctaaat aaaatcgaaa actgaatcca gattggaaac ggagaataaa
    56881 aaagtaaaag ataggcagat gaaaatgcta gaaataaatg taagatctgg aaagtaaaat
    56941 taggaaactg caatttggca aaaggcaaaa caaataaatg ccaaagaccc ggatgtcctg
    57001 aattccggag atagcgaaaa aaaccattaa agtcagtgtc tcttacaaat agccaaacat
    57061 caacttcatc tctccagagt tcatgatgat aagcaatgta tgaaatataa atagtataag
    57121 cagctaatac taataaaccc catatccttc gatttgcttt ttcaatcatg ctatttttcc
    57181 tttgttgctg tatcaagttt atataaatag aactcttctt cttttataaa ctcatttcga
    57241 ttcttagttt tatacaaaag tgtagcacct tttgtaaatt ccggtaatgc actacttgct
    57301 aaataataaa cattctctac atttttaaac ttttccattg taatagcata ggctttttct
    57361 ccctccatct cctctccctt ccatgctttc ttatcccaag gattaaaaga accaaatcgg
    57421 tcagaggcag gaaaataaaa actatgaatg gaatctagat agaataaaac tgttttaccg
    57481 ctatcagcgc tatgagtcgc aatcttattt tctatatcat ctaacttttc tttttttatg
    57541 aattctgcca tttcttttgc attgctaaaa gagtagagaa agtctttttt ggcagtttga
    57601 aatgaaaatt gtatagaaat caataagcta aaaataaata gaacttgtaa tatgttttcc
    57661 gattgataaa attctttctt tctgatatcc aaaatgaggc tatttgatat tatcctctct
    57721 gaatatgtat aataaatcca gtatgcaaat agaaatgcaa ataatacaaa accagcatgt
    57781 ctataacttc tcatatagaa aaatgtatag aacaagaata atattgccat aacccaaatt
    57841 aaaaagaaaa aaggtttctt tgttttatag aatactctga aagcaactgg caaaataaac
    57901 acacttggaa gtattaaaaa attaaactta ctaactccag tcgaaaagga atacaataat
    57961 gagactggaa ttgctagaaa atttttcatg tttgcaaaaa tggatttatg ctcacctgtc
    58021 tctggattag gtagaacttg caagatcaat agaagtcctc caagcaccat tatcataaat
    58081 ccaattaaaa tatttttttc aaattttcgc tcttgtataa tctctaaaaa gtaaataaat
    58141 gatatccctg atgctaaaat ggagcagtat aaatttgtat tgcatagaaa cagaacaaga
    58201 gacccatata aaataggttt ttcaaatcgt ttagtataaa atgatgcagt agcaaataag
    58261 aataaaacgc ctacaccata atttctagaa ataattgaat attcgaataa aggaaaataa
    58321 ctaaacacaa acaataattt gatatagtca ggaaatggac tataagacaa aaataaataa
    58381 acactcgcaa ttgcaatcga tagatgaaca atctgcattg taaatgcagg aaagtttaat
    58441 ttggcaaaag gcagtaaaat aagatgccaa agaccaggat gcccagagtt acttaaataa
    58501 gcaaaaaagt ctttcggaga actaacgtca cgagccataa gccagacatc aacttcgtct
    58561 cgccaaagtt catgatgaaa ggctatcatt gtagtaatca gaatataaag agaaagcaga
    58621 aaatattttt ttactattaa attatatatc aatcctagac tatgcatatt ctttgttagc
    58681 ctacttcaat tgaggacgta tccattctgc tatctcttta gcgaaggagg catatgcttc
    58741 tttcgttgga tgcccatcgt ctactatatg taattttccg tttggatttt ttcgaaaata
    58801 ttcttttgca tattcattga ctgcaaaaaa agggagagac ctttcgctcg cccacttggt
    58861 ttcgaactct aaatactttg cggctaactc tgccatatct tttttaaaat ttggatccga
    58921 agattgaaaa gaaagactca caaaataaac tggaatattt cttgccttgg tctcacgaat
    58981 tatatattcc aaataaggca cactcatatt ccaaactttc tctctatatt tagctgtgaa
    59041 tttataacta ccagacaata ttaattctct ttgttctttg tcttctgaga acatagtaaa
    59101 ttctttttga tttttcttca taatttgaaa tagttctgat ttttgctttt tcttggagta
    59161 ataaagggaa tagagcattt gaacaaatct agatttaaat agccaaggat aataagaatt
    59221 ttttactaaa aaatttggat tttccaaata aggggcgtta tcataattag cttctctaaa
    59281 tctatcttca taaaaatcat tatcatacaa accaataatt aaatagtctg gtttaaattg
    59341 cagataacgc tctaaattaa atgcatagta aattgggctt gctccttgca ttccacaact
    59401 taatacttca aactttgttc ccaattcttg ctctaatttg gcgggtatcg tatctggaat
    59461 ttcagaccca tgaccctcta caagagaatc accaacaaat acaatacgtt ttttacttgg
    59521 atccgatttt aaattatact cgggggctct aaatccaaaa gagttaattg tataatcaac
    59581 agcataatcc gctctagact gcgtatactt tgctaatgga atatgtgagt agatatttgg
    59641 acgaaaatta atgatatatg ggtttgcttt ataaggatct atcttagaag gggtaggaat
    59701 ctctgttaag ccactacctt gaaaaaaaag cctcggacca atttctaata taattgatat
    59761 aaaaccaaat aaaaatagaa ttagaataat tgaaaaaacc ttatacttca tcttatcatc
    59821 tttcatattc atatccctgt tccccaaaat tgtatttgat atccaatgtc cgagatttct
    59881 tttttttgaa taatgttttt ttattcattc taattataga gtgaatgact tctataaaaa
    59941 gagagttata ttcaaagcga actataaact ccatacactt atagaaaaat gatcaattgc
    60001 attcggattt tggatcgtta gttttttaga ctctcccaaa tagaaataaa gtagctaatt
    60061 tgaattagcc gcttgctcaa tggtaaagtg gactacctgt ccaaatgata attccaatgt
    60121 atttccatcg gggtctttaa tatatgccca atatccaaca ggcggagccc attcgtttgg
    60181 accatccaat aaaataccat ccgcttttgc caattcacac aatcgattta catcttctcg
    60241 ggtcttgcaa gcaacaccta aatgacattg aggaagtaat ctaccctcta ccttttttga
    60301 tttcaacaag acaatcacaa aaggtcttgt taaatcactt aaccagacaa catctacgtt
    60361 tatatttgca tctactcttc tgtgaacaat ttgcattccc gcatatttct gataaaagga
    60421 aatactctta tcaatatcca aaacgtctag ggcaatatga gttaaaccaa aatcagacat
    60481 agaaagtctt attcggattt tttgcctgag gaagcgttag gtgcaacact gggaatcttt
    60541 ctgtccttta attggtaaat atttaaatcg gttaatggaa agatcagcca tttcttattt
    60601 gtgtatccat ttactttatt aaatgtgtat agttgacctt tttcagaatc gatttcattg
    60661 gcatctggaa caatatttaa gctgatttgt gggacctgtg tccattctaa aaagttttta
    60721 gcagtatctc ttgccacatc ggattgaatt tcacccaaaa ccaaattaag acccttggca
    60781 tcaacccaaa gtaagaaagt agttcttttg ttattggaaa tgactgattc aatatggtca
    60841 aacatagaga tacccataac gcaatcttct ggttttaaat tttccaaaac ttgacttagg
    60901 tcagcggtta aggtagataa ttcatttaaa tgaaaaacat aatcgtttag acttccaatt
    60961 tgtgtatgtt ttttgaaacg aagatttcct agaatatcgg ttaacttctc tggttgaatg
    61021 ggaaagggat ggataaactt accttgaatg aattcttttc ctttaatctc atcttttgga
    61081 atacgataga aagcagaaga tctggttgaa taaaccaaat gctttgtttg gtaaaaagaa
    61141 ccacaatcaa taataagaaa aattgatatg agtaaaattt tagatattaa atatttcatc
    61201 tatccaacct atttatttgc aatcaggata aatctctata aactcttcgt atgtatagat
    61261 agcaattata ttatctaaac ctgataagcg aaaaacggaa taaagggatt tatttaaatt
    61321 aaaaactgta acagtagaat gtagattttt tatataggaa gcagcacgta ttaaagaacc
    61381 aatcccagat gaatccataa aattaacctt atgaaaatca aagcttacca cttttacttc
    61441 tgaatttctc tggataattc cttcaaactc tttgtaaaaa tctttagaat tatccatcat
    61501 tacatctgtc agtatcttaa tgataagatg gtcgttggtt cgaattgagc ttaaaatcat
    61561 gatctaaata aattatctta caatgtagaa tacatagcta tctaagtatt tatttttaaa
    61621 ttgtctagaa taaaaaagga tttaatcgat tttttgaagt tcttctggct ttacattttt
    61681 aatcgtatga ggatgtcctt ctttatcatt gtagataaca tgatataaac cgctctcctc
    61741 atctacaatt tcaacaatcg aattcgttag tataaacacc ccattttgca tgaaagtttt
    61801 ttttagtagt tttgcctgat caccaacttt cattgcgata cttcctcagc tttcatataa
    61861 atatcttttc cagctttctg cattacttta aaaatatgaa tttgattcga atcaattcta
    61921 gtacctatat catagatagc atctgtaaaa attgctcctt ctatattcgc accagccaat
    61981 tttgcccaac gcaaatctgc tccagtgaaa tctgcatttt gaagattgca attattcaaa
    62041 aaagctcctc ttaaatttgc tccagcaaag tttgccccaa taaaacttcc attttgtaaa
    62101 taggcattgt gtaaatccga tttagagaaa tcgacaccgt ctaatttttc tttctctaga
    62161 atgatactgg ataaattaac agaagaaaga tttccatttt ctttcaggag ttctaaggct
    62221 tttgatttag taattcgttt ttcttttgga ataccagcac cagttcgata atctttcaat
    62281 gcttgtctga gagctgctat cgctgactct ggatagtttt ttcttctttc gggaaactca
    62341 aaaagttttt caatatctga ttcgcttaaa ctctctgctt ctgaaacagt tttatttttc
    62401 gcaacttctg tcgccatcgc gagagccgca ataccaaacc cacaacctgt tgttgtgtag
    62461 ctagcatctg ttatccgctc attctcatca atctttaggt agattctata tccatcgcca
    62521 catcccacat ttctatagtt cgagactaca gttgcatctt ccatctcacg atagttcatt
    62581 cgtgtatcat tgatttcttt gaatttttca aagttcattg ccatttgtaa aactcctgaa
    62641 tgttagactg aaggattaga taaataagtt ccccttggca ttgctcgggg aactgacgaa
    62701 cggacatcga gcgaagtcga attgtcctta tttcattttg tatttctttt tgaatttgtc
    62761 tacgcgacct gctgtgataa cagtttttgc tctctcggta aaaaatggat gagaggcact
    62821 agaaatttct agtttgataa ctgggtattc tttgccatct tcccacacca ttttttcgtt
    62881 agattttgct gtagactttg acttgaaagc atagtcacaa gaaatgtcct taaaaataac
    62941 atctctataa tctggatgga ttccttcttt cattttaatc tcctttaatt atgaattcat
    63001 acttgctaag aacgcttcat ttgtcttagt cgttctcatt ttatcgagca ataattccat
    63061 actttcagtg atgctcatag gggaaagtac ctttcttagt acaaagactt tttgtaaaat
    63121 atctgaacgt agtaagagct cttcttttct agttcctgac ttatttatgt ctattgccgg
    63181 gaatatacgc ttatctgaaa gttttctgtc taaatgaacc tccatattac cagttccttt
    63241 aaactcctca aagataacct catccatctt agatcctgta tcaatgagtg cagttgcaag
    63301 aatggttaaa gatccacctt cttcgatatt tctagcagcc ccaaaaaatc tttttggttt
    63361 gtgtagtgca tttgaatcta ccccaccaga aagaatcttt ccagaggtag gaattacttg
    63421 attgtaagca cgagccaaac gagtaataga atcgagaaga actactacat ctcttccatg
    63481 ctctacaagt ctttttgctt tttcgatgac catttcagca acttgaacgt gacgttgtgc
    63541 tggttcatca aatgtagaac tgaccacttc tcccctcacg ttacgcgcca tatcagtcac
    63601 ttcctctgga cgttcatcaa ttaataaaac gatcaaatat acttcaggat gatttcgagt
    63661 aattgcattt gcgatacttt gcatcaagat cgttttacca gttcttggtg gagcaacaat
    63721 caaacctctt tgacctttcc cgattgggat catcaaatct aaaatacgag tatccatatg
    63781 agaaggatca aactccatat ttagtctttg atttggataa agcggtgtta ggttatcaaa
    63841 taacgctctt ttatttgcaa cttctggagg gacaccattt actgactcta cgcgaagcat
    63901 agcaaagaaa cgttccgatt ctttaggagg tcgaatttgc ccttcaatcg tatcgcctgt
    63961 tcttaaacca aaaagtttga tttgagatgg ggaaacgtag atatcatctg gtcctggaac
    64021 atagttataa tcaggtgatc taagaaatcc gtaaccatct ggaagttttt ccataacacc
    64081 ggctgcatgc acttgtccat ccttctcgga ttgactttgt agaattgcaa aaatgagatt
    64141 ttgtttttta agaccgttag tattctcaac acccatgctt ttcgcaagct cggtcagttc
    64201 gcctattgct ttttgtttta acttaactaa gtcaatgggt tctggtgtag gaccttcaaa
    64261 tttctttctt ttcttaaaag gacgtgagtg ggaaccattt ccattttcat tggttggagc
    64321 agttgtctgc tgttgtgctt caaaatcttc ttctacttca attgattgtt cttctttttt
    64381 agatgccata tgggctgcct gttagtggat ttattagatt tctaatgatg aggcttaaaa
    64441 gaatgtttgc ctatttcatt agcaaagaag ttatttcttc ttttttgatt tagtttttac
    64501 ttttaccttg tttttattga gttttgcctg cgaagatttt ttagatgttt gagaaggttt
    64561 cttggaagat acctttgcga ctttcttact ttttgcttca gtagtccgtt tactttcgga
    64621 ggctaaaacc tttctactag aatcatcttt tttttctact actttttttc tttttcttgg
    64681 aattggagta gaaatttctc ttccgaactt ttcattgatc ttaagtagtt tcattttgtt
    64741 ttgcaaagta gatcttggaa tcccaagctc attggcagtg tgagagatat tatcatcatt
    64801 cttttttaat ttctggaaga tataatgact ctcgacttct tcaataacgg tttcaagcgg
    64861 tagattcata tgaaaagctt cttccactga cggaaaggta tatccctctt cattcaatcg
    64921 gttgattcta ttttgcgggt caataaaatg atataaaaaa ccgctgatgt tttgatcgtt
    64981 tttcaaagta ataatacgaa tcactaaact taaatctttc acatatgctt gcaaaactgg
    65041 aacagtcgat tttctagatt caacatctag ctcatgagat tttaaatagt ttgcaaataa
    65101 atcctgattt aactctctaa aatatttctc tgtataacta atcgagtctc taaaaatact
    65161 tctagctaga atatctactt cgaatcgttc attataaaat gtagtcacgc cttctttatc
    65221 tgttgcgaaa agcgcatcag gaaaactagc tagaacattt gacataaact taaaaatggc
    65281 tgccttgctt tcggtcatct cctcagaagc agactcattg gaatcagttt taattgaggg
    65341 agactcatgg ttcaatttgg aaacttctgc tagaaatcga ggcttttccc aactatccac
    65401 tttttgagag aaaaggttta aaacaggtat ggttcgatta ttttgaaaat agtaaataac
    65461 accttcagta atatgaaaat ctagaaactg ctccggaatt ttttcatagt cttcatttgt
    65521 ggtagataca tccgacatct ccatcaaaac tttttctttt gatatgagac ctatgatatc
    65581 agagttttca tctaccacag gtagatgact aacaggtgtc accatgaagt gcttgtaaat
    65641 cgattctatt ttcattgtag gattttatac ttatgtaata gagtgccaag cactttcaag
    65701 cttatttttt agcgggtgtt actggcttag attctttagc tggtgtatga gttcctcccg
    65761 gaacttcttt tgcttctgtt tcaggcgaag tagaaaagga tttcgacaaa tttgcatatt
    65821 tagcgggtaa tgagtatttt gcgtgatcaa aggctaatac agtataattc aatgccctct
    65881 taaaaagttg aatcgcatat tgaagatttc catctctttc aaactttgta gcatttgcat
    65941 aatcagcttt tgctatagaa tagtattcag atgttttttc cttggtagca gtttgagaga
    66001 tggtaggttt ggtttttttt ccgccttctt tttcttctgc tgcagtcact tgggaagagt
    66061 aagattccat taactctttt gtttccgctg atataaaaac tgcataatcc ttttggacat
    66121 tggcaagttt tgactctgcc aatgttaagg tttttttgat tttatcttta tctcctgaac
    66181 gataggtggt atctacatcg ttcatcatat ctttcaatcc ctggaaatct ttaattaact
    66241 tttcggaaat aattatttcc tgcatagaaa gaagtgtttt tgctttacta tcaaaagctt
    66301 tttttctttc tcctaaatct gcttttgggt ttgcagataa actaaaactg atcatcaagc
    66361 atattaataa aaaatatttc atttaattat tccttcttct ccattgggtt tgttggtggc
    66421 gttgggctca cgggaggatt tgtatttgta gctttagctg gttgagtcgt ttcagagcct
    66481 gctcctgttg taggagaagg tttagcgtta ctgtcaagag tcactggacc tggtgaatcc
    66541 tttcgaataa cgttatttgt ttcatctaag atagaaatac gattgtaggt aataatcgca
    66601 tagcaatcat ccaaaatctc cattaggtct aagctgcctg tggtatttgc tttaaactca
    66661 taatccgttg ccccttttgg acgtggaaaa gtcacggctg tcgaaatttt tccttctatt
    66721 aatttattct cgataaaatt tcgaagagtc gttttcatat attcataatc atttactttt
    66781 ccttcgttga ccgaattgtg taattcactc aatgactgtt tcttgtagat cggcttatcg
    66841 tcgttagccg ttttataatt catcattgca agtaatgtaa agcgtctagc ttttctagat
    66901 gcgcggaatc catcttcata taaagaaagt ttatttctaa actgatatgg catttgattc
    66961 catccgagtt ggtaataatc ttctgcaatt tttaattctc taaacgcttg ttttaacaaa
    67021 cctttagaac tatagtcatt ggacttgatg attctttgtg ctgcatacga gactaagacg
    67081 cgtgtatgct cggtatgtct ttcgagtgca tcttcataga gttcttttag ttttccttgt
    67141 gcatccttaa gtggtttatg tgcatcccca taatcgccgc gaaaatagag caggcttccc
    67201 tcaaaatctc tctgattcgc atctaaaaat tttgtataat aaaaagaatc atctttatct
    67261 atcactcttt ttgcggcggc ttcaggcttt attttattgt ttgcataatc tgaaagtttt
    67321 tcaggctctg cctgaggttt gattaaattg ctcaaaacaa cattaatgaa ttcaaaatgc
    67381 attttgtttt cattcatcaa tacattcaga ttttccatat ctggggcaac cgcatttaat
    67441 gataaaaatg cagagaaaag agaaattaga atcaaagttt ttagtttcat tgcttacctg
    67501 tgaaattggt ctcgttttta gtatcggcga aaaaagtcat tctataaaat aatctagtca
    67561 ctctatcgga cagatttaag gggttatcat accttaacaa cccatctttt tatttttatg
    67621 ggaaattgtt ctcataaaaa ttatctactt ctaggtctta tgattccttc atgagcacaa
    67681 agctttactt tccaaacatg ctcaaatact tccttcgttt gtgcaggagt ttgcctaagg
    67741 gcattggcaa cacgagaaga tgtaaatgta tctttgaagt cacgtaaggt cattttattt
    67801 aaatctttat ttggaaaact ttgtttaaat tgattgagat agcgcgtaac tccacctggt
    67861 ccttggttat aagcagccac tgcaactatg cctcttactt caggcgttag gtgaacgcgc
    67921 tttccattta ctttccaaga ataatccata taataacgaa gaagcatagc agataataaa
    67981 atattagttt caatatttaa aacatttcca agagtatttt ttgcatgggc aagtgtttgt
    68041 aagtaaactg gatgtattgc ggcgtattgt cttggtgcta aatttccacg actcacatga
    68101 tctgtgagct ttcgaattaa atttaaatcc tttgcaaaac tgtgcccttg cttttgaagc
    68161 tgattggcaa tttctcttgc aaatgtttca ttaattctct ttgcatggac aatattttta
    68221 ggaaaagatt ttagtggagg aaaatcggga gtaaacccca tctccttcaa tatctcattc
    68281 aacttaggat ctttatagac tcttccaata tgcgcagttg cagactgtaa tcgatcctct
    68341 gtagatttcg attttcgatt tatctgaagc tgtcgaatag ccacactagt taattgcccc
    68401 aaaccagccg cgcccgtttt acttctaact ttaaaatcaa attgagactc ttgaaataaa
    68461 agacaaaaca atgttggata tggaatttga taataatgag aagctgtgtg gtttgtaatc
    68521 aaaatccgaa gtgctctatt ttcagaatat ccatatatag ggtctgacgt catttttatt
    68581 acctgttccg accaatcact tttcaatgca taatcgatta attccgcaat atctctctga
    68641 gaggataatt ttgttactcc tttattgaaa tgggctttac taatcgaatc atagctaatt
    68701 gcaaaacgat gcattctttt catattttca ttatgaagag gagttttttt cttttttata
    68761 aaatcagaat agaattggtt agcaatagat agaatatcag aggatgcgat ttgttcttcc
    68821 tgatttttat tctcctctac ctctatcgtt tcttttgcat cttgcttacc ttcatcagaa
    68881 gattctttta tatccctctc ttcttgtgga tgaggcgatg ttatacaaat aaatattact
    68941 agtaataaaa ctaaaatctt ctttatgctg caaacccatt tcttcatatt cttttactgt
    69001 cctgtattga ttccaatctt caaagtatag gatttatgca gcaattattt tttgacataa
    69061 aaactaacga ataatcatta atttacaagt ccgaactctt tggattatac atacaaaata
    69121 tatcatttac aaattgctct aaaaataaaa tattagaagt agaatcatat atgaaaaact
    69181 tcaaacctac atacgaagaa ctagaaagaa agattttaga attagaaaaa caaattctaa
    69241 cattcaataa gaagtcttct atggatgatt tatcaacgaa tattgagtct ggattaaaac
    69301 gattcgaaaa agtgaaagaa ctaactgaac ctacatacta caacgcactt attacaaaaa
    69361 attataaatc actcatcgtc tctcatttat gcctttgata aagatggtaa atgcatccta
    69421 ttaaacaaga accttgcaga attatttcaa acaaacttaa atgaagcaat tggaaagaaa
    69481 agagaggaac tgatcctaga taacaataca aagattcata gaaataatga tcttcagatt
    69541 tttgaatctg gtatttctat gcaatttcaa gaagaaataa gttttcctga tgggattcat
    69601 acatttttga cagaaaagtt tccactttat gatgaagtag gagaaattta tgcggtagcc
    69661 ggcatatctt ccgatataac aaaacaaaaa aaggtagaaa aagaaatctt tgagaagcat
    69721 caacttttag aagcgatttt tcaatctgcc ccaatggctg ttttatgctt ggatcgtgaa
    69781 ggatttgtaa ctatgtggac accttctgcg gaaaaaatgt ttggctggag agaaaaagat
    69841 attcttggga aaaaaaatcc tatcatacca acagaaaaat tagaagaatt tgaagtcgca
    69901 catagctata tcatcgaggg aaattccctt ttaggacgtg aaatcaaaag acaaaaatca
    69961 gatgggtctt tactcgatat atttatatca gtatcaccat tgaaagacgt taacggatat
    70021 attttaggtg caatcgctat ttgtaatgac atttcaaaca aaaagattct ggaaaacgca
    70081 ttaaaagaaa gtgagaaaaa gctaagatca gtttttgatg ttttggatgt aggaatcaca
    70141 attactgata aagaaggtaa tataatcgac tgcaataaag cttctgaaaa aatcttaggg
    70201 attacaaaag aggagcattt aacaagaaat tatgcaggaa aagaatggac gatcgttcga
    70261 ccagacttaa ctccaatgcc aacagaagaa ttcgccagcg taagagcctt gagagagaat
    70321 agacaagtag ctaatgttga gatgggcgta ttaaagccga ataatgatat atcttggatt
    70381 ttagtaaatg caacaccaat ggagaatact aaccttggag ttgctatatc gtatatcgac
    70441 attacagatc ttaggaaaac agaaaaggaa ttaagtaaga atttaattga gcaaaatatc
    70501 attttagata attcaaatat cggaattgca aaaactattg atagaaaaat catctgggca
    70561 aataaacaaa tgtgtgagat gtttcagtat acaccatccg aaattctaaa ccttccaaca
    70621 aaatctttgt atgaatccga aatggatttc gaaaagctcg gaaaaattgg atatgatata
    70681 ctaaaaactg gaaatacttt tactacggaa gaaaggatga ttcgaaagga caaatcatca
    70741 atcatcattc gcctaattgg aaaggcaata catccaaatg atttatcaaa aggcagtatc
    70801 tggattctat atgatataac tgaggaaata aaagcaaaac ttacgttagt agaaagtgag
    70861 aagaaattaa aggaattaaa tacagcaaag gatcgatttt tctctatcgt cgctcacgac
    70921 ctaagaagtc caattgcaag tcttttaacc tcgttagatc ttttaaccga tccagaaagt
    70981 gatttttcaa tagaggaaac acatgagttt ttacttaaac tgaaagagtc atcagaaacc
    71041 acctattctc ttttagaaaa tttattacaa tgggcaaaat cacagaaggg agagataaaa
    71101 ttcaatccgg aagaaaatag tctgaatgat ttgataaatg aaaacataaa gttattttca
    71161 aatctttcta aatcaagagg aattcatctt tctagtcagc tagaaaagaa tcatttttgt
    71221 ttttttgata aaaatcttat agatacagtg cttagaaatc taatcaataa tgctttaaaa
    71281 tacacaaaag aaaatgattc agtcttaata agtgccatcg aaaaagataa taaggtgatt
    71341 attagtgtta aagacaccgg aacagggatg aatgaaaaca cattagaaag tctttttcga
    71401 atagacgtta aacaccatag ccaagaagga actagaggtg agaaaggctc aggtcttgga
    71461 ctaattcttt gtaaagaatt tattgaaaaa cataatgaga aaatttgggc ttattcggaa
    71521 ctaggaaaag gaagcgaatt tctatttact gttcctattg tttttagata aattgaagct
    71581 ccgatcaaaa aggatttttt gattgtagaa tttagaacct aacgcacaag tgtaagaagg
    71641 gatgaatatg caaccagata ttttacatac atactaccaa gctctcatgt gtgatagcga
    71701 ggaacaatac gaaaatctta ttaaagattt atctgatgtt gtaaaaaaga acaaggaaaa
    71761 cccttttgct tataataatc ttggacttgc ttattctgaa attggaaggc tagaagaggc
    71821 agagcaaaat tttgcacact ctcttaaaat ccaaaaacac ccaactccct ataaaaatct
    71881 aggaatgatg atggaacgga atgaatggct tgatcgagca cttactgcct ttaatcatgc
    71941 gattcaactc aatgaactag atcctactct tctgcgttgt agaggaagtt tgtatgggaa
    72001 actaggtcgt tatgaagaag caatctttga ttacacgaaa gccattgccc ttgatcctaa
    72061 ttttgcctat agctatttag aacgatccaa tctttacaaa gccatcggca aagatgaatt
    72121 agccactgaa gatctatcta aatacgatgt attgagtagt atttgaataa taataataca
    72181 aactacttgt catcccaaat attccattaa aacaaatgta aggtcatcgt ctaatcgctc
    72241 tttacctccg gagtattcgg tgacagagga aaagattttc tccgaaatgg cataaacatc
    72301 ttccttagca gaatcgagaa tcatttgaat cattctttct tcagaaaata actcagaaga
    72361 atctgccttt tttgcgtcaa tcactccatc tgtataaata acaatcctat ccccaggagt
    72421 taatcgatat tcaacctcct ctgcatctgg ctctttaaga gccattatga tttttccgcg
    72481 accatttaaa cgaattactt ctccattatt tttgatgatc attactggaa aatgtccggc
    72541 tctagcagtt ctcaattttc ctctagagag attaatatgg attaggctcg ctgtgataaa
    72601 gttcttacct aatttgtcat agatagattt atatataaaa gtaagtgatt ccatcaattt
    72661 atgtgtatgt tcataccaaa atgttagaga cattttcacc attgctgcaa gtaaagcagc
    72721 aggaacgcca tgcccagaaa catcacagag aaaaaatcca aagctatttt tgtgcttatt
    72781 gtatctcaaa tcaaagaagt caccacctaa cttcatcatt ggatgatact taacggctat
    72841 tttcaactgc ggtatatccg gaattgccgc tggtagtaga gaatcttgaa ttcttccagc
    72901 catttcaatt tgcttttcca tttctctttt ttgctcttca ataatcaaag ttcgctcaat
    72961 gacttttctt tctagttctt gatttgcaat gtctttcatt ttctcaattg tttttaaatt
    73021 ttcaatcgca gaattctgag caatagatct ttctcgcata acggtcactg caaagtaggc
    73081 aattatagta acaacggcga tatacatatc taataaaatc agggaatcat ttaaagagac
    73141 tttttcattt aaatagactg caaagggtcc ttccccctta actgttttat acacagcaag
    73201 acccgataga aatactatag atgttgttgc tgcatatttt ttgaatctaa gacttgccca
    73261 tacaataaat ggaaatggta aataagctaa aggaaattta gctctgaatg ctaagattga
    73321 tgaaacaaat gtgcatacca aaattacaag taactccatg tatcgataaa atgggaacct
    73381 catatcgggt ggttcagacc aactcagtaa aaaaggagct acaataataa aaccttgcat
    73441 ctcacccgca caccaagttg ccaaaacatt ccaatacaca gactcaggga caaacccaaa
    73501 aatgagaaga gaagtcactc cagtgaatgc actaaataaa gaacccggaa gagtgcatgc
    73561 atacaaaaac ttcattagat ttcgaataga gtcaaagatc cgttcagatc ctacaaggcg
    73621 catcagtaaa aaataattta taataacacc aaatgtattt ccaaatccta tgagaagcga
    73681 ggttggaatg tgcggattat tcatgtaatt cgcaactgtt gcacctataa aaattccagg
    73741 tatagcagac tttccaatta atacgatcgc agtaaatgca attccctcag gcggccaaac
    73801 aggagatact tgcgaattta aaaaggctag aaaaaatcca agtttcgcgc ccaatacata
    73861 actgatcgca attaaaaatg tttttaaaat ttgtttagcc atagctccag tttgaaaagc
    73921 tattatatag tcattgagta taaccttgtc tagtcaaaaa tatttttcaa tcaaagactt
    73981 tcttcgaact tcttattatt cacaagctgg gtagggaaaa aaatcacttc gcctcgtttg
    74041 agcggaacct ttggaatcat ccaacccacc cctgcaaaaa aaggcaaaat atatttacta
    74101 aaaaacatta ctccagggaa atcaaatccg gtcgtcgcaa tgataatatt tcctttataa
    74161 cctgatttaa aaatttttcg aactaaaaac aagccagaag tttgtgtctc cattgtaata
    74221 tcactgatta ctgtatggta atttcctctc tgaaatttct cccatccttc cttcgcatca
    74281 taagctcttt cggtttcaat tttttttgaa tcgaaaaaag cctttagatt attcgcataa
    74341 cgatcattat catctacaat taatattttc attcataaac cctttcataa aatcctgcat
    74401 aatttgaaac tccgagtatt gcttgtgtca aaggatggta agtaaattca taagaaccgg
    74461 gtcctcctgg tcctccaaat acattgattg tgcttggagg taaaaatatt tgccctcccc
    74521 cttctggttg aaaataaagc ttagcatctt tataaaagac agttgctttc tttcctgtga
    74581 gtgggttttt ataatttcca acgtatcttt gatacacttc ctcactagga taagtatctc
    74641 ctgttttgtg aatcggtatt tcggatccaa caatagcgga agctaaatcg cctaacttcc
    74701 attgcataat gactagttga gtcatcaaat tatcatcata ctcctgaggg ttcgacaagt
    74761 agaaaactgc tacatcatac ttaggaaaca tttgcaccca tgcagccaca taatttgctc
    74821 cccctatgtg aaaaaaagtt gttacacctt cttcatccct ctctgttctc catgctaatc
    74881 cacaatattt ctttgtttta gcaaatgctg gaagcggcgt ttgaacttga atcattttgt
    74941 taacagtttc aggttttaga acttgaacgc ctgtaacaga tttcccttca tttagccaca
    75001 tagacgcata acgtcccata tcaattaaat ttgtctgaat tccgcccgcg ccttgcacta
    75061 tgggactagc aaaagatgca tccatcccta tttttgcaaa aatttcttcc tcaatgattt
    75121 gctttaatgg cttattataa actttttcca ggattacacc caaagtcata aatccataat
    75181 ttgaataacg atagtgatat cctgctggat aaatttgacc tgtaatatta aaaggaagcc
    75241 ctgtaatgac agatggaatg acttccggat tctggtaata gcgtaaatct ggcatacccg
    75301 aagtatgaga aagcaaatgt cgaatcgtaa ctggctttga ttttaattca gatttttcta
    75361 attttaatcc aggtatatat ttactaacag gtgcatctag ttcgatgaga cctttctcga
    75421 tcgcttgcat caccattgtc ccagtaaaag tttttgtgat agaaccaaga ccgtagttgg
    75481 ttctatctgt agtgcctagt ttgtagctat aaactaattc acttcccttt acgactccaa
    75541 cagcaatgga tgggaagact tttgaagaat aattataact taaccaccct tcaagacttt
    75601 gtttaattct agcgaccttg gtttcgtcta ccgttccttt cttttttaaa aatgccgttt
    75661 ggtatttttc gatagcagtg tctgtctgac ataaatttac aataattaag attagaaata
    75721 atctaaactt aatcggcatc tggaaataaa tcttcttggt cttctagaaa acttacagta
    75781 gccagttctg tttccggttg cattggcaat ttatccaaca gttctaaata ttttgtagcg
    75841 agagcattca tttgtccctg ataggatgag aaacctgctg ctttataaga agggggattg
    75901 cataattgaa tgataaaaga tttagtctgt ggcattacgc gtaaatctgc aaaaattcca
    75961 ttccacaaac cagtatgata aaacatcttt actaaattat tctcacgcgt tacccgccat
    76021 cctagaccat agtattccat gttagatgtc ttaggcataa aatcaggtgc cttaaaaatc
    76081 tttttaattt tagaagactg gataatcgat tgatctttgt attttccatc tcttaaatat
    76141 acggaagcaa aattagcaag gtcccttgca gatacagcaa tacctgatgc tccatctgca
    76201 ttggaactta ccatagaatc atccatatcg agtgggaaga aaatattttc gcggatataa
    76261 gttgcatagc tttgcccaga aactttttct ataatgttcg ctaacaaacg atagttgtaa
    76321 ttggaataca taaactttgc accggctgca taaatctgtc gtgcgccaat gtatggaatt
    76381 ccggaagtat gattcagaag atgacgaact gtaattggtt ttccttctat tggaggaaat
    76441 tgtttggcat attcaggaat atatttttca attgggtctg ttaattttaa cataccggaa
    76501 tcggatagct gaatgattgc catactcgta aatggcttcg taacagaagc tagagagaga
    76561 ggagtggaat ttttaaaaac ttcatttacc tcaaaaacta ggttttgttt gtaataaata
    76621 gcaacggatg atccctgaat ataattatga ctgactgcac tttgtgcaag tgctgccatt
    76681 gctttttcca atttaactag ctctttgcga atttctcgtt cagatgcttc catttcttgc
    76741 tttttagtgg agagcttctt agcagtcaaa tcgggagata agccttcatc ctccggtgta
    76801 atatttccca caatcggaga taaaaatagc attatggata taaatgtgtg tcgtagtttt
    76861 gaatttttca aggttagaat ctgcctatat ttagtagatt atagatattt gagacataat
    76921 tttgatgtag tatagttacc tcttacgtat attatcgaac cttgtacact gtcaacccat
    76981 ttaaatttgc aatgtttagc tcaatccaag ggaaatggcg atattaccct attcctatcg
    77041 aatctcttgc agaggaatct aaaataattt ttatgaattg cttgctttcc aaatagattt
    77101 ccgatactgt aaaagattca agacctattt ttgatctgcc aacccagtta attttaaaac
    77161 aaagattcca tctattctgt ggtttggagg tttaaaaacc tttatgaatt caaagcaggt
    77221 ttgattttta ttattccaga gaggggtaag atatggagtc tagctttaaa ccaaaaccaa
    77281 ttctaaatcg aaatgagcta agacagatca aacaatctag gactcgcgta gaagggaaaa
    77341 aagtaattac agatgatgta agaaaactta aatctttaaa ggtcacacca gatctcgccc
    77401 cggatgaggc tattaattac tacaaagaac caatctggat tgaatactac attcccaaag
    77461 agtctaggtt tgctctcgag acaaagtatt tgtatattct tttaattcca cctaccccac
    77521 gcccaattga tgaaatttta atcaaggctc aaaaagagga cactttcatc aacctactcg
    77581 aactttctaa ttcaaaagaa aaatacgcag aagttataaa taaatcctac cgaaatagtc
    77641 tttcgatttt agagactatc tcagacaatt tagatcaata caaagaatcg aataatgctg
    77701 agtttttaaa aattgcagtc tatctgacag aaacactcat gttaacagaa ccaactctcg
    77761 ctacattaga agtattagga gaatattctg tacacaattt aatctggctc attaaacgct
    77821 taaataaagg aggagttttg ttttcactag aagacagaac gatatctctt ctaatcaaaa
    77881 ggcgaaatga gttttttgaa gaaaatgatt atcctgttga tgatgatttt gaattgcttt
    77941 ctgcactttt ttttgaacaa gcttaccctc atagaggtgc agaagagtta caagctcagg
    78001 acttcctttt agattaatct tatcattttt tttctctttt aaaaaaatct ttggttgatt
    78061 tttaaaaagc gtctgccata atctgcctct gtctttttag gttaaaggct tttggagaaa
    78121 ttaatgacat ttttgcactc gatatacgat tcaatttatt tcaattatct atcgttcgga
    78181 actctactca gctttatttt tactattcta cttggtatct acttaattac tctcccagga
    78241 aagtctaaga gcacatttca tttaggcttg ggctttgttt ttatgggttt atttaatcta
    78301 tcttatgtat ttgcagcaat gatctatcaa ccgtttgcag cgtttcaccg atggggttct
    78361 gttggattca ttttaccagg aattcttcat ataatacaat ggatctttta ttttcctgaa
    78421 gactctttta ctaaagtaag aaaaactttt caatatacac aatggataat tgccatcatt
    78481 gtagaaatta tttttatata cgttagttta cattctaata ccaaatttca tttcacaggt
    78541 cactattggg actttgatgc agaatgggta agtaaagttg taggaatcgt tatctttggt
    78601 tatattttat tagtccctgc agttggtatt tggaaaacgg tagtcgttaa gacccacgaa
    78661 agatgggcta ttctacaaat cacaattgca atcatggttg cagctattgt tccagctttt
    78721 atgaatatac taagtagaga tgggatatta gatagaggta tatttttaac gtcttatgtt
    78781 ttatttacag tatttggatt tttcttcgta gtgattattt ttatcaatga aacaaatgat
    78841 agaaccacat tcatggcaaa gattgttgga gtaactttgg ttactttctt attacttatg
    78901 caaggaatga gtttcgcttc tatgcaagat aaagaagtgg aatacgataa tatccgaatc
    78961 gagaacattc atcgctatct agaaggtgga acaaaacatg cggatatgca gtatatttta
    79021 gatttgaggc aggagaatcc tgaagtgcaa aagactgatt atgaagatat tgacaactta
    79081 gatctagcac tcattcaaca ggatttcaaa aatagtttag tatacgaaca gataaaagaa
    79141 cttcctgttg aaaattttca aaccagttta gcaactcttc tttctacgac tcacgaagag
    79201 tttagtggtt acaaaaattc aattttggaa tttgtagaag cgaataaagg tttgagtaat
    79261 gaagatctta aagcaaatct attcccctat ttcgaaaaac taaacaaact tgcctttata
    79321 gctactacta aaattggaaa ctttgcagat aaagactttt gctcggaaac aacgaagtat
    79381 ttaaaaaaca ataaagacct tatacaattc agagatgtca tttttgttca tctacaagac
    79441 tgtaattgga atcgaagtta cctagagcca gcaaaattaa aaagagaaat caataaatat
    79501 ttacgttact ttaagccggc gatgacaaga cattatcgaa aaagtataga tggaagcaaa
    79561 cactacgtag cctatattca ctacaattca gcaaagaaaa gtataactga aattggattt
    79621 tcttacattg cttacaggaa atatttacat cctgcagcca aatctcaaat tatcatatta
    79681 tttattgttt tagttattat tttaggcgta ttcccactct tctttaaagg aagtttaatc
    79741 aatccactga tgagtttact cagaggagtt gcaaaggtga attcaggaaa tttggatgta
    79801 gttgtaaaag ttaaagtcca agatgagatt ggttttttat cccaatcatt caactctatg
    79861 gttgattcaa tcaagcaagc tcgaaaggaa ttgcaagact atgctgataa ccttgaagaa
    79921 aaagtaaaag aaagaacaaa agaagtgcaa gagaaaatgg aagaagttca gaagttaaag
    79981 atacaacaag atggagacta cttcttaact tcccttctcg caaaaccgct tttcttcaat
    80041 gcgaataaat caaaatatgt aacaacagat tttatcattc accaaaagaa acgatttgaa
    80101 tttagaaaca aagttgcaga tctaggtgga gatatttgta tcactggaaa tcttaaattt
    80161 ggaactcctg aaaaatttaa acgttataca atggcaatga atggtgatgc aatgggaaaa
    80221 tcaatgcagg gagcaggagg atctcttgtg atgggagtcg ttatgaattc aatcatggca
    80281 agatcagccg caaataaaag gattttagaa ctgactcctg aacaatggct aactgatgtc
    80341 taccatgaag taaattcggt atttaagtcc ttcaatggaa caatggttat ttcagcaaca
    80401 gttctattaa ttgatgatga atccggggaa atgctttatt ggaatgcaga gcatcctttt
    80461 acaattctat atagagatga aaaggcaaac tttattgaag atggtcttag actcaggaaa
    80521 ctaggtcttg attctgaata tgaattcgcc gtttataaat ttcaattggt tccaggtgat
    80581 atcgtaatac tcgcgtctga cggtagagat gatattgatc tgactccaaa tgagccggtc
    80641 agaacgatta atgatgatga aacaatgatc ttatctgtcg ttgatgaagc aaaatgttca
    80701 attttagaaa ttgagagaat tctacagacg aggggagatt taacagatga tttatctatc
    80761 ttaagaattg attacaaaat aaatatgcca gctgaggaag cacaaagccc aatgtcactc
    80821 actccatctt ttgaagagaa ggaaattagc aatcaaatca atgtaaatga aatctattca
    80881 cagagtaaaa aattatatca agcgggtgaa atagaaaagg cgttagagtt attggcgaat
    80941 gcatacacag ttgagcaaaa taatcaaaaa ttaaataaac ttttaggtct actcagtcta
    81001 aaaggaaaag attatgatac tgctgttaga gttttaagtc aatatctcag tcaagatcca
    81061 gataccgatg agatttggta ttacctttct atcgcacaga agaaaatggg caactatctt
    81121 tcttctcttg aagcagcgaa gaaggtttat gaattacaac caaataacat aagtaatctt
    81181 gtgaatcttt ctgatttata tcgtttaaat ggaaattata tcgaagcgaa aatcttttct
    81241 gaaaaggttc tagttctaga ttccgaaaat aaaaacgcta agaagatact aaatgtatta
    81301 aacagcatga ataattagta tagatagcag ggaatactac atgaatttaa attggttaca
    81361 tttttcttat tactcagtag gagtatttgc agcatttctt tttatattca tactaacaat
    81421 ctttttcata accaaaaaag acaaaactgc tccaacatat tggcttgctg gaatgtttat
    81481 tggttatgat tttatgctac ttggatatgt tcttgcatat tcaatctcag agccatgggg
    81541 tgcatatcac agattcatta ctatgtgcgc actctttgga aacgctggcg tgataggttt
    81601 tgtttatttc tttcctcgct tagatacacc gagagaggct aagattgtta tatcactttc
    81661 tcttattata atatttttag cctattttga atttgtaata acaggttcca agttggagaa
    81721 gatttataat ttcacagcac atttttatac cttcgattac ggtgcaagac aagctgtaat
    81781 tctactgctc acacaattgt tacctctttc tgtaatcatt cgcaaaacaa ttcggtattc
    81841 tgaatacgat ggatattttt caaaatggct taaaaaacca gcagctatta gcggttatcc
    81901 tctccacctt ttttctagat tcattgttgg ttggattaaa tttttcaatc ccaaaggaaa
    81961 agatgctcat gcatgcaaga gtttcgtaaa ggcaatttcc ttttttatag taatcgcatc
    82021 agccaatcta ctaaataaag ctggaatcat aaattatgat ttatattcag cgatctatgt
    82081 aaatgtaacg ctcatactta ttttctattt ctttattact tatatcaaca attcacctga
    82141 gccaactact ttcatggtaa aattagttgg ggtaagccta attactatct tacttgtctt
    82201 aggttatgtt ggcaatatta ctctatcact tagtgaagag gattatgata atcaaagaag
    82261 agctcaaatt tttggtgcta agcagaatat tgtagaagga aagtatgatg aacttccaga
    82321 agagattgaa tatattttag caaagcctgc aatggctgat atattcgata aaagctataa
    82381 aatcgaattt atcaagaaac ctgatgttct aacggctgag atgattaatc aaggcgcaga
    82441 tcgtttcaaa gagggactta ttcgtgagac gcttacaaaa ctcatgaagg aaaataagag
    82501 agatataaaa aaagataaac caacgaatga agaatatgag gaagcaatca atcgcttcaa
    82561 aaagtcacgt caatatgata acattgccaa tgaaaatcta gtagatgaga cgaatcaaaa
    82621 aatgaatcgc ctttatagag atgctgtaat tctttacaca cattatgatt tcataaagga
    82681 taatgtaaaa tatgaaattg gattttctta ttctgaatac cgaaagcata cgcaccacaa
    82741 tgctctaaaa ttaatctata ttatttttgt aacaactgtt ttagtattaa ttacattccc
    82801 ccgattcttc gcatcgagtt tagtcaaacc tctcaataat ttacttgggg gagtgacaaa
    82861 agtaaatgaa ggcaatctag atatagtagt tccaatcaaa gtaaatgatg agattggatt
    82921 tctatctgcc tcttttaact caatggttgc ctctatcaaa caagctagac gtgaattaca
    82981 ggactatgct gagaatttag aggacaaggt tgctgccaga accaaagaag tgcaagagaa
    83041 aatggaagag gtgcaaaaat taaaaattca acaagatgga gattattttc taacctctct
    83101 tcttgctaaa cctcttttct ttaatgcaaa ccgttcttcc tctattaaaa ccgattttat
    83161 catccgacaa aaaaagaaat ttgaatttag aaataaaagt gccgacttgg gtggagatat
    83221 ttgtatcact ggaaatttaa aatttggaac aacagaaaga tttaaacgtt atacgatggc
    83281 aatgaatggt gacgcaatgg gaaaatccat gcagggtgcc ggtggctctc tagtaatggg
    83341 agttgtaatt aatgctatca tgtctagatc cgcttctaac aaaagagtca tgaatacaac
    83401 tcctgaacaa tggctttcag agttatacta tgaagtagac tcagttttta aatccttcaa
    83461 tggaacaatg gttatatcag cgacaattct attgatagat gatgaaacag gtgaaatgta
    83521 ttacttcaat gccgaacatc cattcacaat tctttataga gatgcctgtg caagctttat
    83581 tgaaacagaa cttcgattaa gaaagttagg acaagaatct gaatatccat ttgcagttta
    83641 taagtttcaa ttaatgccag gagatgttgt gatactagga tcagatggaa gagacgatat
    83701 tgacttaacg cctaacgatc caatcagaac aataaatgag gatgaaaatg caattcttgc
    83761 aactgttgaa gaagctggtg gaaacattta cgaaatagaa aatattctaa aaacaaaagg
    83821 cgcaatcact gatgatcttt cattcgttag aattgaatat gctcctgttg aatctcaggt
    83881 atcatccatc ccagagactt atcaaccgat aacagaaaag ttagagcaac ctgaaattga
    83941 aatcaaagaa gatgccgaac tacaaattga tgtagctgaa gtttatcaac aaagtaaaaa
    84001 actataccaa gatgggcagg taaacaaagc actcgaagtg ttaatgaatg catatgctgt
    84061 agaacaaaat aatcaaaaac tgaataagtt attaggacta attagcttta aaggaaaaga
    84121 ttatgcaaca gcagttgatg tattgagtcg ttatcttcaa atggatccag acacagaaga
    84181 gatgtggtat tatctttccc tctctcaaaa aaagatgggc aagtatttag aatccttgga
    84241 ggcttctaag aaagtgttcg aattacatcc agagaatgtt aataacctag tgaatctttc
    84301 tgacctatat cgtttaacgg gtcattttgc tgaagcaaga gaattctccg aaaaagcact
    84361 tgcactagat cctacaaatc aaaatgcaaa aaaaatctta aactacctag gcaatgtcta
    84421 aaacttgcta atagatcaag attcaggtat tttgttgaga acaatagact caacaaatac
    84481 ctattgcaaa aagaaagaag tcccaatcgg ctactgggta ttagccgatg aacaaacggc
    84541 cggaagagga aggcatggta gagtttggct ttcacagggt gatgaaaaaa tttttttctc
    84601 agctaaattc acattagaca aattagaatc ttccatttct attttatctc tactgattgg
    84661 gagcgcagtt ttaaagactc tccaaaacta ttttccggaa caaaaaaatc aactttcctt
    84721 aaaatggccc aatgatattt accttggaaa caaaaaagtt tctggtatac taatcgagtc
    84781 ggaatcaata gaagaaaaaa cagtttttat cgcaggaatt ggaatcaatc tatatggaaa
    84841 agaaaaatta gaaacgagtg actatgtaag tcaaaaaccc attgggaagg attttaaaca
    84901 gaaacttatc ttttcactca ttgcatatat caatgaaata gaatggatcc ttcagaatac
    84961 aaatctactt agtaatgaaa tccaatggat ctatgaaaat tctctactga aaaataaaaa
    85021 aatcgaaacc actgacaatg gaagaaattt atctggtatt gtgactggtt atgatagaaa
    85081 tggtttctta attgtaaaaa taactgacgg gaacttagtc tctcttatgg atacaggtcc
    85141 ggattttaag gtgggtttat gaacaaggat atgttgctgg taatcgatgt gggaaataca
    85201 aatacggtct tcggagtttt ttatggtgaa gaggaagaac ctagattcca caaaagaacg
    85261 gttactcgaa gagatagaac ttccgatgaa ttaggagtct ttttaaaggg ctttttcaaa
    85321 gaagaaaaca ttgattcaaa tgaaatcagc gctgcaatct actcaagtgt agtccctggc
    85381 tttaatccaa ttgtttctag aatgttagaa gattggttca aagtaaatcc aatcagtgta
    85441 acggttaata tcaatttgcc attcacaatc acataccctc gcccttttga aataggagct
    85501 gatagacttg tgaatgctgc cgcagtagta agactctatc caggaagaag tatcattatt
    85561 gatatgggaa ctgctacaac attttgtgtt gtaaatgaac atcatgaata tattggcgga
    85621 cttattgctc ccggacttaa aatttctatg gactctctca ttcgcaacac tgcccaatta
    85681 ccgcctatcg tttttcaaac acctagaaaa gttttaggag attcaacaat tgaatcttta
    85741 cagtctggct ttttctatgg atgggttggc cttttagaag gcataatcca agaaataaaa
    85801 agagaatacg gtcaagacta caaagttata ggaaccggtg gactgattac aacaattcac
    85861 aactcccgaa aaggtctttt agactatatt gacccgctcc ttactttaaa aggattaaaa
    85921 cttttatacg aaatgaatcg atagagaaat gatttatcag gaaggctttt gcagtgtcgc
    85981 ctgacgactt ccttcctttg tttctgctcg acgcatctta acatctactt cgagagattg
    86041 tctaccagaa cctttgaacg ttcctcttac aggggaaaca tctcggaagt cgcgaccaag
    86101 tccaacacgc atatgcttct cagatgaaat catattattt gttggatcaa gcccaaacca
    86161 tgaaaaatct ggaatgtaaa catctaacca tgcatgactt tgctctgcac caaccatttc
    86221 aggctgttta gaatcagggt cataactttc tatatacccg ctaacgtatc gagcagcgat
    86281 tccagcgctt cttaaagccg caatcatagt atgagaaaaa tcttgacata cacccattct
    86341 tttttgaata acttcatcta ttggagtatt aatattcgtc gcgccagatt tatatttgaa
    86401 ttcttttgta aaaatttgat tcaaatctat aattgcgtca gtgagacgtc tctcgggatc
    86461 taggattttt tttgcgaagt ttctgacttc ttccgtaatt ggaacaaata tagatgggtt
    86521 tagataatca taaaattcta actggtattt atcaaaccat tccttcgctt ctttaatttt
    86581 cataagagaa ttgagaggtt cgtgcgaagg agcaaataat gttacatcac tagttgcagt
    86641 tatttctaaa tagctatgtc gaaatggaac agaaaattca tgcactaaat ttccaaaata
    86701 atctctatga gaaaacattg gaacgtcagg cgaaacttca atccaatgag aatttacact
    86761 ttgacaaagc gtttcgagtg gagaaatttt aacttgagaa taagattcgg aagcttcata
    86821 atcataagaa tagcgagtgc gatgaataat tgtgtatctc tgtgtcatag ttgcaccgta
    86881 aaaggagttg gagaagaaat attactataa cgatccataa ttttatttcc aagtaattca
    86941 attcccgctt tcaaatgaga tgcccaatag ccaggtttga cttcatcttt cgcatataaa
    87001 aactgatcct gcttttttat tttaggggga tttacacttg gtttaccaag attgtctttc
    87061 aaatcaaaag agaaggcgcg gctaattaat acgtcacaaa tatattctgg agtttcctca
    87121 tcatgcatag taagacctga atctgtgcct atcgcattca gaatagattt aattttgtcg
    87181 agtgaactta caagagaatt attaaaacct ttatccgtaa tcatcatttt gtaggctaaa
    87241 taaggatcaa aaatagattg atagttccat tgatatgctt cataggaatt caatatcttt
    87301 aaaattacta taataaacgg ttgtaggttt ggcatatctg tatcactctg tgtcgctttt
    87361 actaaaaact gaagagtgga ttttatgact gtgataatat aactacaatg ctctatatat
    87421 cgcccgagct gtataaattg taaaatatcc tgtctagaaa aagtatccaa ggatgctcca
    87481 taaaaagact gagaataatg aacaacccca accagccact cagagagatg atctacggtc
    87541 atgttttgat ttactgaatc ctcgacacct ggaagttccg ataaatattc caatagcttt
    87601 cgaagagaaa cccacatatc gtttgataaa tagttctgaa tttctctcgc attattcatt
    87661 gcataattca tattagattt aacagaatat ggattgcggc tatcggctac agcatgctta
    87721 aagaaactca catgccaagg ctcactggat ttaaataatt ttttaacagg tgacccagtc
    87781 aaacgtgata aggttttaat aagacttgtg taagatggat catcgcttcc aagaagtatg
    87841 tctatttcag aagaataggc aacttgtaag acattggctg ttgtaaaacc tcgattgata
    87901 taacgtccaa gccaaaataa actttctgca acacgactta aaattaaact tctagtaatt
    87961 cgatttggga aaaggatttc acttcttctg tggataacat catttttccc cataatccaa
    88021 gtatctttac ttccccctcc cattgcattg caaaccatca gcgaagattc ttctttcgaa
    88081 acacgagtga gagcacaatt actcaaatga aatgaatttc caagaaaagt ataaaacctt
    88141 gtttctacat atctctcctt aaaatttcca tctggagtaa aaacttttgc tgttgaaaga
    88201 ggaattaatt cttgtgctac atattcaaaa ggattcttta ataccttttg tttcatatcc
    88261 tctatctgtg ctctcgttga ctctcttcca attaaaattc caagaccacc tgttccctgc
    88321 ttgggcttaa tcacaaagtc atcaatattt gaaaaaacaa attccacaaa ttttggatcc
    88381 gaaagaagag cagtgggaac agattttaaa ataggctctt ccgataagta ataacgtatg
    88441 attgcatctg taaatggcaa caagctctta gaacttgcca ctccacttcc aattgcatta
    88501 gcaaccgtaa catttccata ccgaacacag gaaaaaagac ccggaactcc aagcacgcta
    88561 tcttgataaa aactaacagg atcaatataa tagtcttgaa tccttctgta tataacgtct
    88621 accctagaaa gcccgtaaac ggttttcata aatacatgat tgtcttttac aattaggtct
    88681 tttggcagaa cgagtggaat ccccatttta tttgcaagta gctcatgctc actgtaagct
    88741 tcgttataaa tacctggcga tagtaaaact accattggat tattatcctg tgtaggcgcg
    88801 cactctttta ggatactcaa catgaccggt gtagtatccc aaatttgttt aatggaagcc
    88861 ttctcgaaaa tatttggaaa ctgttgacga aggacttgcc tgtttgtcat ggcgtaacta
    88921 attccacttg gaatcgtaac attatcatct ataacttgaa aactaccatc ggatgaacga
    88981 gcaatatcaa aagcaccaat atgcaaataa acgtcatgcg gcactttgac agattgacaa
    89041 tctcttagat aatttggatc agctaagatt acttctttag gaatgacttt atcaattaga
    89101 atttttcgac tagaataaat atctaatagg aacttattta atgcaagtaa tcgttgttta
    89161 acacctcttt caacaagaga aaactctgac tcatttacaa tatgaggaat taaatctaga
    89221 taccaaggtc tctcaacata gttccctctt ctactttctc caaaagtgat tccttgttct
    89281 tttaaaacaa attcaattct tttattaata gatctaagtc tatctacact ttgggattct
    89341 agcatcggaa gaatttctga atattcagga cgatatttcc catttgaatt tttaggtttt
    89401 atctcgctga aagattcctt cgactgattt gttcgcacag aaaaagagtt agattgttcc
    89461 gatgtcattt gccagttcat ttaatggtta ttgggttgtg gtttaaagtt gtggttcttc
    89521 ccagagtata agactgagaa attgcttcaa atatataata ttttgccaga ctaattgact
    89581 caattaaagt catccctttc gctaaatttg cagtaatcgc agcggaataa gtgcagcctg
    89641 ttccatgtgg attaaaatct ggaacaaatc tagaactgag tggataaata gttctgccat
    89701 cgtaaaaata atcaactgca tactcagatt cttttaaatg accacctttt aataaaacag
    89761 gaactccaaa cttatcgtat aacttcctta cacctgcatc catttctttt tcatttgtaa
    89821 tcgtttcacc aattagaatg ctcgcttcgt ctaaatttgg agtaaataaa gaagaaatag
    89881 gaatcagctt tttttgaata gcgtctaccg catcttcttt taaaagccta gcaccgcttg
    89941 tggcaaccat aacagggtct actacaattt tataatcttt acccttaagt aattcacaga
    90001 caatatcaat aatttctttt gaaaaaagca taccagtttt aatagcggct actgggaagt
    90061 aaccagtcac tgcggaaatt tgcttttcaa tcaactcagc ggatacttct tgtatgccgc
    90121 taacaccaga aggattctga gcagtaagac aagtaattac tgtcgttcca aatattgaat
    90181 gagctaaaaa tgtttttaaa tctgcttgga tgcctgcgcc tccacctgaa tcggaacctg
    90241 cgatagttag tgcaatgggg aaatctctca ttttgtttga aaccaaccgt aaatagttaa
    90301 atcaatttgt gctacaatat taaatacaat cccctcttgt tcaagcagtt ttcgttgaag
    90361 ctttgctcta attgtatccc ctttaaaaga aatttctcct ttagcattga tcactctctg
    90421 ccatggaatc tcctgctcct gctgtttctt taaggcattc aaagcatatc cgacagcacg
    90481 agcagcacga ggttcgccta aaaaacttgc aatttgtcca taggttgcca cttttccctt
    90541 cggaattttt ttcacagctt tataaacacg ctcgtaaaag gagtctttct taatctcttg
    90601 ctttttaggt ttcacaacta taccttatgc aattaataaa ccatacgtca ccaaaaaact
    90661 tatatattta agtaagtttt gcaaggtatt aaatgaaatc attctaattt ataaataaac
    90721 acattcctat aaatctcttg aaaaacattg caagatattt agtaaactaa aacggtccaa
    90781 agtgaaaaac agacatgttt gtttatatag aatttgagat aaaaaacaac cgacttttct
    90841 aagctacatc tattatcagt ttctaaaact ctaacttttt acttacgctt tacacatttc
    90901 agaataaaat atttaaatgg ttttttatga ggcttacaca cattatccac aacttaaaca
    90961 acagcgaata tgatactaac catatacaat tatgcagttc taatagaaca acagattcat
    91021 cttatatact caccttacat tatcactgct taacaaaagc aatgattgag tttgggtgag
    91081 ccaaacgttc aaatgaagtt tgcgcctagc ggcgggcttt cctcccatcg ctttt
//
LOCUS       DAOQOS010000005        82017 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_90_length_82017_cov_6.354933, whole genome shotgun sequence.
ACCESSION   DAOQOS010000005 DAOQOS010000000
VERSION     DAOQOS010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 82017)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 82017)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..82017
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90_length_82017_cov_6.354933"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(273..1091)
                     /locus_tag="PLF66_01810"
     CDS             complement(273..1091)
                     /locus_tag="PLF66_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016545467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HMZ62798.1"
                     /translation="MSLKSKRLGSLADIFQAEVLEGTIRKIKLSKIQPSEKQPRGERK
                     KSVEELANSIMADGLLQPIVVTKSDSEEQYKIIAGERRFHAVSLLGWTEVECKILNKP
                     EKEVYKLAVIENLQRENLSAYDEAEAMKVLKSEYGYTDNEIGIIFGKSRNYISEILSI
                     SNLGTKELEICRNAGIDNKNLLVQAAQSYKKGDFNKFIQSYKNGTFRTIKEAKEFNKN
                     SEILTSPKSNKNKKSNEHVNIEKFDNKLVISIDDPKLLLKIESKVKSILKKYLP"
     gene            complement(1075..1827)
                     /locus_tag="PLF66_01815"
     CDS             complement(1075..1827)
                     /locus_tag="PLF66_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000637633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HMZ62799.1"
                     /translation="MITISVTNQKGGEGKTTTSINLADGLARRGKKTILLDIDPQSNS
                     SSIYTSKADEEKNMFHVFNNKAKLASIIKETKTNNLFIAPTTQRLAEMEAVSANNVDA
                     PFILRDSMEGLTGFDFCIIDCPPSLSIFTINALVASNYVLIPLQAEKFSVDGIVGLQQ
                     TITSIKKRINPQLEIIGALITQLKQHTLLTKTIIPILTKYFKVFDSCISDGVAIGESH
                     LARCSIYDYNKKCRQAKEYEDFVKEFLDELKK"
     gene            2122..3432
                     /locus_tag="PLF66_01820"
     CDS             2122..3432
                     /locus_tag="PLF66_01820"
                     /inference="COORDINATES: protein motif:HMM:NF014950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HMZ62800.1"
                     /translation="MISGLIKKSMQDPKSFYENVEAFFEGFAQILECNCGILVLQDKE
                     DSFLEVSSFGYLDDWYYSFLARGLGNLEKIISTDNFVYFPSENFKLYNPKSKHGFGYR
                     IECQGKVFGFIFLESVQELSDLKKEFVKIICNDIGFIHLSKTNFTEKKPLDRDEISHD
                     LFAFLLRDSLGFTNTLKANRFINIQGGRGVGKKSLVKFIYNKLGLHGKLIYLNALPDS
                     VEKLEKSIRDWILLAENGILIIENVSSLSISQQRVFFEIIKEPILEPRIIFLDDMMKH
                     REEYQPFWNLLSQHTIRLPELLSLDKEVFREIVNILFYDLRQLHSRPDMMLMDEVYEF
                     LFQYPYNENLRELRNLLEMPLINLKENIITKEFYLKLNNSEIKSLDFYNKEDLDLRKS
                     VQALERQKILLANKLFSGNQIKMAKALGVSRGSLQYKMKQLELH"
     gene            3432..3923
                     /locus_tag="PLF66_01825"
     CDS             3432..3923
                     /locus_tag="PLF66_01825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62801.1"
                     /translation="MLSNQETVEERKTPDGFVVRVHRATMTYEVFSRKGVEIPRNSKN
                     NVSIIKVPRIIFMGTAFNLSHFRLADLTYVNARSGKLQIPYQFSDSSDIRILSVPTGS
                     DSDVLPIIPYQYNSVKEIVHAVDQGLEFQTIIVDESSPKNEMVNLKIRFPNVNVLILR
                     KKN"
     gene            4000..5013
                     /locus_tag="PLF66_01830"
     CDS             4000..5013
                     /locus_tag="PLF66_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016545576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62802.1"
                     /translation="MEEDQQYEERKTPGGFLVKVRLAKLTYVVFTPKGPEVPKGAKNN
                     SIIVPVPRVVFLGDDFNLSHFRLGELSFVNVKSGKLVIPYQHGGSRDIKTIYLNSGSE
                     SDILPVIIYHFNFVKDIMSATEQGTEFHHIVADESLLKNDMVTLKIRYPTVNILILKK
                     KINVAYENKVPANATTAENKSEFATPVVDADIARAADMASSGSNLNMYSENPVFLARI
                     HLRNLELDKVKQLLFDFNLKSEDIIFIRTFLDVMIKNVNNKEELVTNKDKVKVLDEIF
                     RLAILIINHNEAEFELELEKGLSKDVAYMIYALLEKYQEQAKNIEEEIMLWEWKYRAR
                     RLM"
     gene            5018..5320
                     /locus_tag="PLF66_01835"
     CDS             5018..5320
                     /locus_tag="PLF66_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004425467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62803.1"
                     /translation="MSKVAFSKLLEAMKSLNNEVNDQEICRRLETLMLTSKDDLNLSV
                     VNHLLDNPLEFDPKEIPEPYTMYVKHFIYMVKRNEKLGIPTKFSSPSSEKGGKKKE"
     gene            complement(5322..5930)
                     /locus_tag="PLF66_01840"
     CDS             complement(5322..5930)
                     /locus_tag="PLF66_01840"
                     /inference="COORDINATES: protein motif:HMM:TIGR00573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HMZ62804.1"
                     /translation="MILKDYEFTAIDLETTGLKPGQNEILEIAAIRFNKDKPLAEFHS
                     FISPRKELKKDAQLVNGITKEMLENAPSLDKKLAEFFHFIENSMLVIQNADFDLSFLL
                     FESKKLEIPFPNLPVLCTLNMTRSLFPNYPKHGLAHLRKYFKIDTIKTSDRTDFHQAL
                     DDSYAAMKVFIYSADKANLWEKDISILNYHPKGFKTSSDFEI"
     gene            6298..6513
                     /locus_tag="PLF66_01845"
     CDS             6298..6513
                     /locus_tag="PLF66_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010572574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62805.1"
                     /translation="MAETLKVRVRCHACTYMIEGTAKYGKGHYVPEGVDFEFIAIGKV
                     ETSKGRTVKAEVLCICPNCGVKCKFSV"
     gene            6718..8001
                     /locus_tag="PLF66_01850"
     CDS             6718..8001
                     /locus_tag="PLF66_01850"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010572571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HMZ62806.1"
                     /translation="MDFSIAKRIEFSSTRKTKFFLLFISLFFNLSILGFFKYSAFFLE
                     SFFSFSALIGYSLDRIVFNIILPAGISFYTFQSLSYTIDVYRGVISAEKSFLQYALYL
                     AFFPQLVAGPIVLAQDFLPQIKTVFKKNFESINLKEGMYYILLGFLKKSVIADNISII
                     TDFIFSENTNLSSISWQYLLIGILSYSIQIYGDFSGYTDIARGVALLLGFHLPENFNL
                     PYFASSLTDFWRRWHISLSIWLRSYLYIPLGGNRLGNLFTYRNLMITMLLGGLWHGAS
                     WNFVIWGGLHGFYLAIERFFQSRPFIMSLVSDIRPSLKTALSIFYSIFTFILVSVFWI
                     FFRSQTISKVFSILWGIVTLQSGITPSYSVFNQFYFLFFLVLIGHSIGYFASDRIKDF
                     LARKDSYFIFSFYAFLLIVFVLYSGELKPFIYFVF"
     gene            8137..9816
                     /locus_tag="PLF66_01855"
     CDS             8137..9816
                     /locus_tag="PLF66_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62807.1"
                     /translation="MIFINLEESKEKRIFCHLSRFKSIVKFNLESLENGMQESLLPET
                     IDKNIKSSKKNWIQILVFVLSLFLFDQIFFGFFIWNLPNESPWGTNHFFNFIYEKKRI
                     EKTQKVNQRILIIGSSIAYYSLDKDSIREYLHKKLNKDFEIEYLSYAGMTPLDSYLFR
                     NQIELLKPDLIVYPINFIDWRLHRAYVLNPNGSNETADDRSLILDALNFGDAPQSKFA
                     FPMEAILEFHSLLSLDRIADYLSSSTFLFYRYREIFSQNLRNIYNHRFGRNISYHGYA
                     GVQIPERVNSLGWTGRNFSFYPKEYMFERGFLIQIVPEILEKGPLILTLSSQGLEEKY
                     SFQDTGWKKVVLPFKFQSKENKITASLSNVWYPFQAKEDRFDHSRDEVGVRLQQTFGL
                     DRPLSGMQYKREERIEDLRYISMSDEEYKEYFEYRLLSDFEHRPGIRYIHALKNAKER
                     LASEKFRPGIHMQYLQKFLESMKQKKIPILLINNPENPLALRWYEDSQWYKDHLDYLV
                     NLSSENEIFFFDLKHSLKMQDFSDFHHITFPGMEKMNFIYGDLIAELFKEK"
     gene            9944..10648
                     /locus_tag="PLF66_01860"
     CDS             9944..10648
                     /locus_tag="PLF66_01860"
                     /inference="COORDINATES: protein motif:HMM:NF013106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic peptidoglycan transglycosylase"
                     /protein_id="HMZ62808.1"
                     /translation="MLFIPWVPVLYFGVLIYFPYDETGKSRYMRITGPLVQYVGAEWV
                     STDRIPPVCKKAIVVAEDSEFYNHSGISIESIKSSFEYNRRQGRIVSGASTITQQFIK
                     NAFLSRQKTYVRKLREAIGALMFNLLFSKDQQITWYLNVVEFGPRIYGLQSASNFYFK
                     KDPFKLNAKECAVLSTFLTRPVLFGKSYKNGLTSVAFQKRFHRIYNGLVPPPAKKEKQ
                     QPEVIEEEDIPEEDSE"
     gene            10814..11869
                     /gene="asd"
                     /locus_tag="PLF66_01865"
     CDS             10814..11869
                     /gene="asd"
                     /locus_tag="PLF66_01865"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012476745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HMZ62809.1"
                     /translation="MSKVKVGVLGATGSVGQRFIQLLENHEYFEVVALGASDKSAGNT
                     YKEVMKSRWKVSSKIPDYVSNMKISRCVPKETPNVDLVFSGLDSDVAGEVETEYAKAG
                     VMVISNSKNHRYDKDVPILSAEVNAEQLKVLDAQKTPGKIITNSNCTIMGVTITLKPL
                     MDAYGIDSVFLVSMQAVSGAGYPGVPSLDILGNVVPHIGGEEEKVEMEPQKCLGKVSG
                     GKIVPANFKITAHCNRVPVVDGHTVCISVKLKKKTTAEKIKKLWADFSGEPQKLKLPS
                     APKQVIDYREEIDRPQPRLDLMSGNGMTTTVGRLRPDPIFDFKYVVLSHNTIRGAAGA
                     AILNAELIYKKGYLRVK"
     gene            11871..13811
                     /locus_tag="PLF66_01870"
     CDS             11871..13811
                     /locus_tag="PLF66_01870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62810.1"
                     /translation="MSEQNSQFFRVVDFNPAAQSVQGMESRGDFQYKGIYRFFTTINE
                     WTEKFLANKILPNAEQLTRDISIEKDKVDFFINELCFKANPPIIKKITTVEFELSDIR
                     KSENLSTGLRRNTVFARPSALDAGSCQRYINGCNERSVAAIKRAIAEVRVPWHGDKFK
                     DYIFTRINANRLSDTYASYDIGNLFNCPYDSTKQLKEMTVNMHLKPVLKKLVDDKVLL
                     FFRNEKALKSANKSVFLYNSKDEIIERIEIYVNYMKKHIIPSLQKIGVIGGVSDEEYA
                     DFASLSTKILAYMDDSYGDQKYMVEELIILGAFYENYREEIKKKEQKDKVIEVVKLVE
                     NAGKLVDLATIRVNGDPIPKELIPSILANENILYCEYDDGSSLFEFLLHKSAINLAAD
                     LARKIFQSTGNDTDIRILSRMNINTALDENIKKDFYSAEMESLFKYLPFLTRLWRMIM
                     GNIYVTKEEAEQIRRQKEMEQKKRITESKSKQIAKEKSRLVEERMKQIDEIEQAGTGA
                     DAKEEPDKKLTPEEEKQVKDVLQAIITALDSAWDAKLYPDREYLMQNVKTGMSEDQFV
                     MFMKKHSQKEVLSFQIKSKSNKYKWPILVSRHYLKRHGRRMLEIAKRESDTERRSPAP
                     DQDKFDLFSHLEEFLERVLPRI"
     gene            13811..15235
                     /gene="pyk"
                     /locus_tag="PLF66_01875"
     CDS             13811..15235
                     /gene="pyk"
                     /locus_tag="PLF66_01875"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004456332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HMZ62811.1"
                     /translation="MHGVERFRKTKIICTIGPATSDINMIRKLAEAGMNIARLNMSHG
                     DHKFHGEIIQKIKKLNKDLKYPIAILIDTQGPEIRTGEVHTELDLKVGEIFTFHVIPG
                     QESEEKSVFVNYTDIINDLKIGDKVTVDNGLINLVVLEKKEKELVCRVLDGGKLGSRK
                     HINLPGIKVNIPSITQKDLKDILFGLEQDIDFIALSFVRSQEDILQLRKIIEENEAHA
                     QIIAKIEDAEAVKNYREIIASADGVMVARGDLGVEVELEELPIIQRRIIKECAFQGKR
                     VIVATHLLESMIHNPFPTRAEVTDVANAVFEEVDAIMLSGETASGKFPVRCVEMLHKI
                     AMRIESSGGGVGYVHNRRPRNKKEELARSAALLADSLKCNAVIVITRRGTTANNIAAY
                     HPEHPIIHAFTNMTSVRRKLWLNRGVLPYRVDFSSDPEKTIALAIETLKKNNMIARGQ
                     QVVILSDIIAGEDRVETIQVRDVK"
     gene            15402..18197
                     /locus_tag="PLF66_01880"
     CDS             15402..18197
                     /locus_tag="PLF66_01880"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HMZ62812.1"
                     /translation="MKKNVFQFFLAFAITLSLASIVQEEFLDNKEIFPHFKEYLPDTL
                     EVLEKEFKKEPPKLEQVKTNLYAGKDYEYLNSFFEKLSSLERSKDRVIRILHYGDSML
                     WSDMITSEIRQRFQKDFGDGGRGLVPITNQLSRKVLSHKNKTDETQFTWRNLDHRQFV
                     NPQIGFLGEAAIPNYSFVSMRHEIDSEIPWSNVKLFFRSPLPNSQFKSKVLYKVKQIS
                     NTVTLEDIPVKDWEISDSKDCKEMEFVLEDGVSFQLDITGMNSTPIFDSIQFETKAGI
                     SYSTVSRQGIEISDLLLIEEKSLTCGFTRYKPDLIVFQFGVNESENLARKAFITPEKY
                     EKDLEDVIRRFQKILPNTNILLISPIERISKNEKGIFQTMPEILFIRDKQKEIAEKNK
                     IAFFDSFTALGGSGQNDVLYENGFMQPDRTHLTRKGGEFFAEIFYSDFFTHYKKFNGI
                     VEETKQASLEKLKKEKNKEVNFVSKAYFYFFLFVWIVSSLLTRYPEYKIGFLAIVSFY
                     FYSTWNFYAVFIILLSTVIDYFCGLQIYKKRQTGKNGSGYLILSLLSNLSLLFFFKYY
                     DFFAGLLNGVLVNGNPIQIPLLHLILPVGISFYTFQTLSYTIDIWRGVLVPETRMFRF
                     AEFVAFFPQLVAGPIVRATEFLPGLKNRGEHFYLNSIQFSNGVFQILVGVLKKIIADR
                     LAAGIVDGVYANPDMYTSPEILAAIYAYGVQIYFDFSGYSDIAIGSARILGYNLTLNF
                     NRPYTAYSINDFWRKWHISLGSWFRDYLYIPLGGNKSFVYRNLLITFILCGIWHGAGI
                     PFLIWGIYHGVLLVIERGLKIDRKENYLMRLITLHLVLIGWIVFRSDSWLTFQKLIQN
                     LFSFHMNIQNLELWIILFLFAYYIYEWIGFNFINRIQSLWESSSFLFQGVLAGILILI
                     AYYLHRVEIKPFIYFQF"
     gene            18274..18915
                     /locus_tag="PLF66_01885"
     CDS             18274..18915
                     /locus_tag="PLF66_01885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62813.1"
                     /translation="MKYIYYIAPIALVLFIGFSFFSDFTKPKKKNASTALERAEEMYS
                     ESANAKRKKRASGKSLFDADSKFLEFPEIGNEEIDLNAPLTPEEREKRRKFIIEKAKK
                     LADMFPNNSVIPRHLSPDALKKLEETNQRMASIQNQLIQNEKVSKEDKLFYYKERLKT
                     SKDRLEVFRYALGLENNGVFDESKLEGFLKERYDIFVENEKAYADEIIKVEKE"
     gene            18931..21252
                     /locus_tag="PLF66_01890"
     CDS             18931..21252
                     /locus_tag="PLF66_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HMZ62814.1"
                     /translation="MKPALLITQCLQNDFTKLIGKFDPFPNLLHIGYDEARRMLSEKI
                     EEGPVTTVMDWAYQTPSEKLTIIHIRDWHNPNDPLQKDHLQQFGAHCIQNTEGAEFVF
                     AKSILPNRPHHIVNASGLNDFVDTNLEEILSSYKDEPIRVGIMGVWTEAKVTFLAYDL
                     KTRYPNFEIVVCSALCASSSRTMHFISLEQLTSILGVKVFSSIGDFTNFLTGTLPKIE
                     HKIQDSKGIQIKFSDPNYSVSDTDMQILLYLFRDAKDVEFLCLDGGFSGNVVLKAKAI
                     DKYGHTQVPTVIKIGKRELIGKERTSFERIQEVLGNNAPSIVDYAELEDRGAIKYRYA
                     AMLDNGVLTFQKYYAKIDDMDRIKHKLEIVFKRQLGRLYDASTNEKLNLLSYYEYSNK
                     YAASIRNKVETIIGTKASGNTLNLHGYSFPNVCNFYEIDLATLQENITPYHFVSYLHG
                     DLNGANIIIDAQENVWLIDFFHTHKGHILKDLIKLENDILYIFMKIDSLEEFKEAVHL
                     IDILLSVPDLGEPLNESISNQFTFPQIIKASRTIAYLRSFYPDLIHTDRDPYQLFVGL
                     MRYSMHTLSFDESNDWQKKLALYAGSICSAKIKNTLQSTKDLRIDFIENPSNQNEGKM
                     GLTILPGRNDRARSIAEDLRVIGENNITAVISLITEPEYEEYGVASLKEAYKNAGLTT
                     CYFPIIDQGVPDFITLDKNLKWMHEILSKKQNVLIHCVGGLGRSGTVAACYLIRYYGL
                     SAKDAIAHVRKYRSERAIESIEQEKFIEEFANK"
     gene            21299..22285
                     /locus_tag="PLF66_01895"
     CDS             21299..22285
                     /locus_tag="PLF66_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010576365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HMZ62815.1"
                     /translation="MEELVKVEEIQKAKEKINGLLEELKKEITGQTAVIRNITTCLVA
                     QGHVLLEGMPGLAKTLLAKSIAQSIDLSFKRIQFTPDLLPADLIGTVIFNPKTANFET
                     RKGPIFTGILLADEINRAPAKVQSALLQCMEERMVTIGETTFQLETPFMVLATENPID
                     QEGTYQLPEAQMDRFLMKVNVGYPEESEEIAILGQHGSVSAKSKEIKKCLTRKEVQEI
                     SSLSDKIYIDEKLKNYIVKLVRNTRPQTSKLDEIKPYVKHGVSPRASLALLRCARANA
                     LLEGRNFVIPEDIRFSIYEVMRHRMILTFEAVSEDVTVDSLIKTVLEVTELP"
     gene            22309..23130
                     /locus_tag="PLF66_01900"
     CDS             22309..23130
                     /locus_tag="PLF66_01900"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HMZ62816.1"
                     /translation="MILKELEKLIQEIELKYKKKIRSNRAGSILVNRSGRGMDFKESR
                     VYMYGDDIRFVDWNVSSRMGELYIKLFHEENDRTINIFLDISKSMSFSGANSYSKFFI
                     GFQFLAFISLLSVMTGDRVNIVLYSDKIEYVAPGIKSRVDAYRILRRINETTPKGKTD
                     HLLPLQYLKNKIPRNSISYIISDFSGINDLSPYRTLLEIHELYGIRISDPIENMNHPA
                     LKSFFVQNIETGNGGDYSSTYNSDSDILSEFYRSNLLNLRTDSDLGKSIVRFLNK"
     gene            23127..23978
                     /locus_tag="PLF66_01905"
     CDS             23127..23978
                     /locus_tag="PLF66_01905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62817.1"
                     /translation="MKLFILLFLFPILLFAEVIESYSKKSVQVGEESILEIKFQTGDV
                     AEWSLPQKGFLFSESDAETPIGELKDITQTPTELKFTYVYFEAGKFSANVSWKTSSGE
                     TFKSKEELEVKSVLSGEKEPIDIAEPFVFSGSYILRLIGIILFGIGIISAFGYALFFF
                     NNKKKVQAKDAIFEKLETEQAADYYKKQLQQLLLNTEISHKEFIFHLSGYIKERIGAK
                     LDTSVFHLTQTEIHQILQDKYFVANIELLTIDNYFNSVKYMPNEEMITRENALSLIKY
                     WEKILAK"
     gene            23975..24922
                     /locus_tag="PLF66_01910"
     CDS             23975..24922
                     /locus_tag="PLF66_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001011135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HMZ62818.1"
                     /translation="MNYFERPELLYLIPFVILYAFLFYRFKFHENKMKLDLGNQSNVP
                     KFPTLETIKIYFPFLRFIILILLILSLAGPGSQSSLLPDEKNGVDMMIAIDVSGSMVR
                     SNDFLPKNRLEVSKDLIKNFIKKRVNDRMGLVVFGGAAYLQSPLTNDIESLVEIVSEV
                     NQNTVDEQGTAIGDAIILATYRLKTSKARSRILIVITDGVSNIGKIDPITAADTADKF
                     DIKIYTIGIGKDLQGVFETDFDSLRQISEKTDGMFFRATDSNEFTEVLNSIDTLEKDN
                     LNVKPKLFVEPYYMYFLLPAILLFIFDIMIRSFYLRYYL"
     gene            24919..25965
                     /locus_tag="PLF66_01915"
     CDS             24919..25965
                     /locus_tag="PLF66_01915"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HMZ62819.1"
                     /translation="MKNLDPSYFILIVFLLASFCVYLFYKLKQRIEIENFLKVYTGLS
                     SKVTLPPRWILILRYSIYSIIVILLCISILNPSFSEESGADEKIISGVDIVFLVDVSL
                     SMNATDVLPNRLSQFKETVLRLLPSLNGNRLGIIAFAGVPFLYCPMTNDIAAFSDYIR
                     GLDVDMIPNTGTNLKKAFAKAEEIFKSGKVLRNKVLILVTDGEDIKGSTPARLNADLV
                     IWGVGTEEGGGIFYKDEQTGIAGYVTRSGNLIADKSNTDLIRTKLDEEYLLELASIQK
                     ADYSNISAQPQSADKLISKIQSMSKNTSSKLSNMFKKDGYQYFLFPALILFLLDISLL
                     EYLFRKYANMHSNN"
     gene            complement(25977..26984)
                     /locus_tag="PLF66_01920"
     CDS             complement(25977..26984)
                     /locus_tag="PLF66_01920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62820.1"
                     /translation="MSQIEKLLAAHIEYEVSAWKGEKLKSNLQIEVESLYDTIVEINF
                     NDLIDKNYILDSAKNYLQSREIQKELENLAQNLSESTYQTIQNSKEPLTSLLTKSIFE
                     ESMNMGLSMTELRKDVVHNIINSPVYTKMISSVLYNAIAEFIGGENSIAKNVPVASSL
                     FKLGQDFLGNIPGMQANIEKNVTAFIQANLQNSLKQSEKLLNQELDAKTSKELTDELW
                     NFISKKEVADLQHYANKNDIKKILSIAKSELEFIKASPLLIQILEGHIDVIQTQYKDK
                     KIKEILSLYKVEKKDITDNLIKILTKYLSLPEVQSHIQKRITARLEGFYASAEARNIL
                     K"
     gene            complement(27065..27640)
                     /locus_tag="PLF66_01925"
     CDS             complement(27065..27640)
                     /locus_tag="PLF66_01925"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HMZ62821.1"
                     /translation="MLFFSSFIHVSAYAALYIATNTDLDSINIHEDIDLNFEDIPSTL
                     NNNASKPTPVKVKDWIETEKGIKEDSIEENIDLNALSGNGIDKDGYQIPQNAQRAPTP
                     IIDFDLKQFFPTAAMTANITNKTVILSVQIDETGTLKSAKIVSSKIGYGFEEAAIKII
                     HKAKFLPGYAKGKPVKMTHRIPIHFTLDESE"
     gene            complement(27692..28270)
                     /locus_tag="PLF66_01930"
     CDS             complement(27692..28270)
                     /locus_tag="PLF66_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010572405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HMZ62822.1"
                     /translation="MLFLSIFIHFSVYAAYYISTIINIEEVDSDNIKQDMDVNFEEIP
                     PELIGGTSSPAPVEKSEWVEGSNKNADDPIDEDISVNAVSGNGTDKDGYLFSFNGDKV
                     PTPIIDFDLKQFFPAAAKAANITDKTVVVLVQIDETGALKSAKIASGKAGYGFDEAAI
                     KIINMARFSPGYVKGKPVKMSHRVPINFTLDE"
     gene            complement(28325..28729)
                     /locus_tag="PLF66_01935"
     CDS             complement(28325..28729)
                     /locus_tag="PLF66_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HMZ62823.1"
                     /translation="MAMAQQSGDDDEIGNINITPMVDVILVLLVIFMVTANFLKKESI
                     NINLPKVQAADPNVAQSVQVAITRDGKILLEGQSTDEEKLFRTLERDSKLRPNMRITL
                     SADEHLAYGTITKLMGLIRKAGVTRIALSVKK"
     gene            complement(28879..29484)
                     /locus_tag="PLF66_01940"
     CDS             complement(28879..29484)
                     /locus_tag="PLF66_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016545755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HMZ62824.1"
                     /translation="MEQLVDLGEQLVFILMGLASILALAVGFERSVVFSKFTKKGDLS
                     MRGIIDLLRKKDIKGVADLSTQSSNVYSRFAEFSVTHYNDGQEALGEMMHGKVISERI
                     MLEKRLPILSTLGNNAPFLGLLGTVLGVIKAFNGLGTLGNSGAEVVMKSISTALLATA
                     AGLFVAIPVVMANNYFSKKLKVITQNLEILSKEFIASYSRK"
     gene            complement(29585..30082)
                     /locus_tag="PLF66_01945"
     CDS             complement(29585..30082)
                     /locus_tag="PLF66_01945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62825.1"
                     /translation="MSRIFLTVFIILSFSFCSKSNEKDYKDLYALRILLVTATTPRAN
                     PQQACIDAMRAEEACIVTASDSGGLVNETNFSFQISSGRFTTYSDLCANNLYMYPLPA
                     NFVTNNSDSLRECYFRCDRSYWQTRKNLNICTGTIAAMTAGIGTDSGTSTCKRNCISV
                     TNNAP"
     gene            complement(30082..32622)
                     /locus_tag="PLF66_01950"
     CDS             complement(30082..32622)
                     /locus_tag="PLF66_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002973381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HMZ62826.1"
                     /translation="MNSKFYNKYKFLLFVGLLSILSSEVSAVAFRGRLFSRAKNQGEA
                     GLTVVVVTPNAKIPAQTDAEGYFDVEVPALGTYTFRVLRPTGIQELEKAVTADGEVIT
                     LYTDKAPKPKGGIDVEGQKDKTILSRYKVRYDEIKRMPGTLGEALNAIQTLPGVFAPP
                     AIGGGNGGCFGGIVIRGADCRMNTYLYDDLPIYYAYHFDTINSVIHNDLIKTIDIYTG
                     AYPANFNNATGGVVEIESNEAAKKATGSFNMSFLLGQAMYQTPILNGKGYLAAGGKIG
                     YLDKTLGSTGLIPDGIRLPQYNSSNVKFIYNFNANHQISFTSLTALDGFVLNAPGGYG
                     ANDPTKQPLSAVAGASVAAGQGFRTMGLRYIWTPSTKFSNRITLINYEPFNNTTVKFG
                     SIEADFRASAPYSGIRQDLVWNPTSYLKIDFGTEGRIMNYNITGYSIRQRDPNNPAPN
                     PYNLTNPDFEKADITQKTQTKYFNAYMTFHFTFGNFKFEPGIRHDYVDYSKQGASGPR
                     FVASYKFEGILKGTTVFGGAGDYNRYPYFDQAISRESGNPNIQFEKARKYGGGIDQQL
                     TEEWSVKGELFKQEFTNLIVNDPYITEAYGLNPDKYQRLQRPIVTNRALNYSNSGTGW
                     AHGYEIYIKKSNRPNSKDWFGWISYTWSQSFRNNNIYNADYDPNRNYTLSAEQQRLRS
                     LVPNSRELIFDYDITHLLSVVYGWRITEEYQIGGRWFYRTSIPYTPVIGDDGGQFRNP
                     YNSQIYWNPRYSNNPYSNDYSNSRRQIPYHRFDIRIDKFLAYEWGYINVYLELINIYM
                     RKNVNGEDFDSTAPYSKRNPGPRNDFFTLQLPSGMLIPFINVGMEARF"
     gene            complement(32627..32977)
                     /locus_tag="PLF66_01955"
     CDS             complement(32627..32977)
                     /locus_tag="PLF66_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012389940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62827.1"
                     /translation="MKDRLLHSSIILTILFFFFHCNEKPIPAWDAQSQINAAADFKAR
                     ECKQAGPPQPPLLVFTDQDSRNLNLCSIAITRTSCPFLGYPIICLGIYTTEKLGDIPW
                     YLNFNNDLAKRQIK"
     gene            complement(32977..33417)
                     /locus_tag="PLF66_01960"
     CDS             complement(32977..33417)
                     /locus_tag="PLF66_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004473164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62828.1"
                     /translation="MLKKLVNIFILSLLLNCIMVVSYPTNLNRRADNQHITPSATLIR
                     SEGYKILQRETGETSTFFLFGLIPLTNPHNMDYAMSQAVQKVEGGDSMVNMTFWHETH
                     YFYPLGTVSVTKVSGDVVSLKTDTQAPLFEEPSNNKKPQNRKSK"
     gene            complement(33398..33769)
                     /locus_tag="PLF66_01965"
     CDS             complement(33398..33769)
                     /locus_tag="PLF66_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004459659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62829.1"
                     /translation="MSKHIYLILIAFLLNCASSSAGISTSNIPIVNKKYKVISPVEGR
                     KSWVTIDIAILAVSFSTPPINELVQEMINSKEADALINIRYWQDRSIFLFLTWNRLGI
                     NAEAVKFTDEPQTEPNAKKTR"
     gene            complement(33795..34322)
                     /locus_tag="PLF66_01970"
     CDS             complement(33795..34322)
                     /locus_tag="PLF66_01970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62830.1"
                     /translation="MKTKKKISKKNDKLSFDPQMMSFVNKMGKYYESFGISRIGGQIV
                     GLMLITDSGITQEEIQRILGVSRSSISTNLKMLIHNSGIEEVRISGERKSYFRISDRA
                     AELSIQKKLNSYDYLKEVVAEAIENLKKKGSSTEKLIGLLHWINLDKEVSQELLEKWK
                     SILNSSAQAGRKQSK"
     gene            35036..35608
                     /locus_tag="PLF66_01975"
     CDS             35036..35608
                     /locus_tag="PLF66_01975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62831.1"
                     /translation="MKYLLILLFSLSLAAQSAKPVTVKTFDTVVDSKTETKKEKEKPK
                     EKEIEPKESSSLDDDELDKLFYTYYYLNLPKHLQKEDKDKKKLHTNLIGVTKKEIAKR
                     DRSAPKEDIDNISPSTVKFKRVTEESKWMKKIKAEMKYINFTEYMYIVRYKHYLVLII
                     FHLDPELHLQSPAQVEFLNVQKTEQAQDLD"
     gene            complement(35618..36103)
                     /locus_tag="PLF66_01980"
     CDS             complement(35618..36103)
                     /locus_tag="PLF66_01980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62832.1"
                     /translation="MYTPLYKIFFLVGIFTLLVFTGFIFLVQDREKELIEAYKVLERE
                     GQKSFSTLTGYHTVIIGNQKRHIYTYMVTDEFGKLHETTEYVDDKTHLRLRVGNTVIT
                     RQKKFKLAGKEKILSRIDGNKQILPNYNFLKRIGIFGIGFSIFINVTGSFFLLFKRKM
                     S"
     gene            complement(36150..38291)
                     /gene="scpA"
                     /locus_tag="PLF66_01985"
     CDS             complement(36150..38291)
                     /gene="scpA"
                     /locus_tag="PLF66_01985"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: protein motif:HMM:NF006944.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HMZ62833.1"
                     /translation="MEKANLGDWEKVAKEADLKGKDVSTLNWVSPEGILIKPLYTYED
                     LENLGNTNTLPGIFPFIRGPRATMYSHQPWTVRQYAGFSTAEESNAFYRKNLAAGQKG
                     LSVAFDLATHRGYDSDHERVKGDVGKAGVAIDSVEDMKILFDQIPLKEMSVSMTMNGA
                     VIPILAMFIVAGEEQGAKISELSGTIQNDILKEFMVRNTYIYPPKPSMRIIGDIIEYT
                     SKNMPRFNSISISGYHIQEAGGNAVQELAYTLADGMEYVRTALARGLAIDDFAPRLSF
                     FFGIGMNFFMEIAKLRAARFFWADLIKQYNPKKPMSAALRTHCQTSGWSLTEQDPYNN
                     VIRTTIEAMAAVLGGTQSLHTNALDEAVALPTEFSARIARNTQLIIQNETHIPRVVDP
                     LGGSYYIESLTHSIIKEAKKLFEEVEAHGGMANAVEAGLPKMKIEESAAKRQALIDQG
                     IDVIVGVNKFKLKSEDKIEILDIDNTAVREKQIARIKEIKAKRDESAVNAALNAITEA
                     SQSGKGNLLDLAVDAARKRATLGEISFAMEKVFGRHQAVIRTISGVYSSLNQDNEKFQ
                     AIIKRVEEFAKKEGRRPRMLVAKMGQDGHDRGAKVISTSFADMGFDIDVAPLFQTPNE
                     VAKQSIENDVHIIGVSSQAAGHKTLIPELIEILKKENAGDILIVAGGVIPPQDYDFLY
                     EHGVAAIFGPGTVIPDAAAKILDILEKRVKV"
     gene            complement(38295..38615)
                     /locus_tag="PLF66_01990"
     CDS             complement(38295..38615)
                     /locus_tag="PLF66_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004788068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62834.1"
                     /translation="MRKKLALVISISLFLNLSISAKEVPSSGEMIAEILVRPLGLVGL
                     ATGASLFIVTLPFGVLNRISTGSWETVTNSGKRLVIAPALFTFARGVGDYPGHMEEIE
                     LVKE"
     gene            complement(38612..39622)
                     /locus_tag="PLF66_01995"
     CDS             complement(38612..39622)
                     /locus_tag="PLF66_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000345462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HMZ62835.1"
                     /translation="MYLQNINHELILFIIFNIIIVLMLAIDLGLLNKSSNHTMSFKRA
                     TVWTLIWVTISFLFAAFIYFYDTDPSDPERSKTKTMEYIAGYLLEYSLSIDNLFVFIM
                     IFQKFKVGPKEQPDILKWGIIGAVVLRAIMILAGATLIAKFSWILYFFGFFLVYTAIK
                     MFVHKEDENETYIPEEHLVFRLVKKIVPLSTRSEEGKFFILENGRKLATPLFVVLVMI
                     ETSDVMFALDSIPAVFSVTQDAFIVYTSNIFAILGLRSLFFMISGIMDLFVYLKHGVA
                     IILAFVGFKMLLPLYGEIMHVEKIHISIQVSLAVIVSLLVGSILVSLPEYLKNKKSGV
                     AK"
     gene            complement(39686..41227)
                     /locus_tag="PLF66_02000"
     CDS             complement(39686..41227)
                     /locus_tag="PLF66_02000"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HMZ62836.1"
                     /translation="MKKVIYYLFAYNLFVTQVFAEDTLKFNLKLDNAFQNKNLLHTIL
                     YFEDQNRIYDINKISESITNSISAWNSTGEKELSFGFTKSVYWIHFSIQNESTKLDRL
                     YLECNLATMDNIELYFSDSSQAFYNKKVLGDIHPFKEREVKYRTNVFLIPVKRGEETK
                     IFLRLENKGPMKFSLFLRSPDEFSAHVVRDQVFLGIYYGIMIVIFFYNLFLYLSIQEK
                     TYLYYIHYVASVALYQFIISGNAAQFLFDNAPEIANRAPNIVANLSFITAMLFTRSFL
                     NSEHYTKRFDKILLLLTGISTLLLIIILILGHEMILMKVSNLLGLAKIISIISTAIQV
                     YRIGYRPARFFLFGWSFLLVTIFIQIMANLGAFNITAEHFGQIGSAIEVILLSLALAD
                     KMKANSQEPVSIPSQTEIQNETESEKKPDTNPKETVRRPLFTRLQVEYGLTYQQSQIC
                     CALVDGKSRTFIAKELDISLNTLKKHLSEIYNKTINKIDTNEIPSQEKLQKLTVFLHS
                     LKPKD"
     gene            41593..42972
                     /locus_tag="PLF66_02005"
     CDS             41593..42972
                     /locus_tag="PLF66_02005"
                     /inference="COORDINATES: protein motif:HMM:NF019237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HMZ62837.1"
                     /translation="MREKVLDTSENIYTGKNTYLFFLVCLVFTQCIHNNPNTKLIAIL
                     NLKSQTVISYSKPTFEWVRGTAILEEKPRIQRLNPINNDINFQFSIDPELPKGLVFDT
                     VTGTISGKPELAQIPTEYKIKISNNSESQTIPLRVTVYGYLPLKTNQTTCSNNVGTAI
                     SCSGTGQDGELQMGREADMNLPTAPSEYPEDYITKDNASGLVWKTCPYGRSGADCNTG
                     STTTVDWSNAQSICRDLNNANNGNGFAGYKSWRMPTREDLISMATTMGLSSVVSNTYF
                     PNSIGQSWTSKELDDLNAIRALGVSLVISTSKSNLTPIRCVTGNYNPKPAYLDNNDGT
                     ISDYSTRLLWQKCAVGLSGDNCDIGSATGMNRGNALTVCNNLSLVGKKWRLPNYNELL
                     TLLKPDEFSNPLDLSYFPGIQLNSYWSSTNHTGSPISGYSVNFTSGTSVGGLKTSTTT
                     SVRCVANMD"
     gene            43105..44064
                     /locus_tag="PLF66_02010"
     CDS             43105..44064
                     /locus_tag="PLF66_02010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62838.1"
                     /translation="MENLKIKESEKIFWNLHGKILSDQYRPNEIKFFLKTVDNQCFVE
                     ILEYSQILNEDGFLELLFYYLWLFRDDRDINLIINQKGFPTKYLIKYIFSSMGRWISQ
                     GNDVDDFFNVVGLQFSSEKSLELLTKERVEQIDISLGLVLICNLKERDLNRFLFEIHE
                     TDYIVNLFIEVFDQLEEDRIKGFFIKNPPLFATLVNILKEFSIKKGLSKFEKFIKRFQ
                     AEVEVIENLISAARTIHCFYDVHSEKKKNLFERDMKRIIQIVMILDDTKFLQEKLELL
                     FSESVILDSEEIHLIENIITNPYLKSILIKKEDLEDFENEPRE"
     gene            44048..44203
                     /locus_tag="PLF66_02015"
     CDS             44048..44203
                     /locus_tag="PLF66_02015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62839.1"
                     /translation="MNQENKKILPMQAEDKTQNEKIKELIEIAEKFRMTKKKNIEEQT
                     VILKKAE"
     gene            44217..44969
                     /locus_tag="PLF66_02020"
     CDS             44217..44969
                     /locus_tag="PLF66_02020"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HMZ62840.1"
                     /translation="MINEHSELTFFQGMGFVEKEIEYTIERDSLYRLILHDLKNPVLG
                     IYSLARHLQEQPNTDVKKMHQYNGMILSASESLLSLIDSFQIFLHAETQDERNTFKYT
                     NLNGLISSAIASVCANAERKNIIIHCNLSSFDTFYGSEFFTSVILRNLLTNAIKFTPK
                     DGSILVTTSVRGAFFEVSIKDSGIGMTQTDIGKLFNLKKIFTRLGTEKEKGTGLGLFF
                     CREFIYKQNGTFSIESVPGLGSNFIFTLPLIS"
     gene            44994..45275
                     /locus_tag="PLF66_02025"
     CDS             44994..45275
                     /locus_tag="PLF66_02025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62841.1"
                     /translation="MNKKKKVVVVALLMTVGNIQSIERYDKKPSKDLKELANELNRSP
                     DPYSEKDKYKSLCDKDGYPLVGNTASKGDIPPRVEKKVYLKVQEFCKTK"
     gene            45278..46042
                     /locus_tag="PLF66_02030"
     CDS             45278..46042
                     /locus_tag="PLF66_02030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62842.1"
                     /translation="MAFQVFNPTQIQNFPWEKRLRKKKAWIYELPFDEEIEPTDSSKL
                     VWLRTAYSEYASAAAFTNISQALLACAAPLDMVSCVNDFAIDELIHTEVAILVANKLG
                     CNYALEIDYNKLVRPASKENASILKACELIVRSCCVGETLTVPILKKSAELAKSELIS
                     KTLTKIRKDEISHAQFGWYFLDWAEPFLNQSDLNFLGNVTRETIDSFQIVLNAECSES
                     GYGVLACQDFDPIFLSAVQKNVILPLKERGIIVEID"
     gene            46195..47898
                     /locus_tag="PLF66_02035"
     CDS             46195..47898
                     /locus_tag="PLF66_02035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62843.1"
                     /translation="MKFKRKVFRKIRKFLFCFLLLSSFHYCNCFLYKSGKLKGSMALS
                     GSSQSGGVLNDTTTYSISGNISGLTSEGLVIVNNTESLTVSANAQLFQFSQKLKKGDL
                     YKVEIKSYPSSPPQSCLLVNASGTVQSADISNIEINCSTGYLVSGVVSGLNSSLVLQL
                     NGGSDLTVMANGSFSPFASPLANLMNYSVTVKTHPTGQVCYVSKGEGAITSSNVSNIE
                     VQCTNGNLPPGPLVGGSIVKELIPQYGNDNPSLGSPFVGGIGVANAIDGASTSARFSG
                     PYQITTDGVYIYVSDRDNNRIRRVRTSDGFVDTFVNDGNNMISQPTGITTDGVDVYVA
                     TQSKCQIVKIKISTGAISIIGGGAAYTCAIFNLPLAMTIDTNNLYITSFGNNKVMRLD
                     LSNNSISTIVGTGVSGNTDNSIGTIATINGPRGIIKVGNYLFISTGGHNIRKVDLTTG
                     TYSTSTLAGSTTGVNGAIDGIGTSSRFFSPFAISSDGTNLYVADSGNKTIRKIVISSG
                     LVTTLAGCDSDPAGTTIGSGNPSISCAGAARYREPSGLTSDGFYLYVSDHLDHIIRRV
                     E"
     gene            47971..48390
                     /locus_tag="PLF66_02040"
     CDS             47971..48390
                     /locus_tag="PLF66_02040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62844.1"
                     /translation="MLLACFVKKVDGIKYFKVNKEDLTFNSITLMQRNTVSSNEEEQN
                     IILEELQIIKDRYSLVMEVKDNIIELYEEECKKINSNVANEIKNITFKTKKGNFPITE
                     FSMIRFSKELKNNFPCKFEKENPEKNCYKIYYEEIRI"
     gene            48387..49712
                     /locus_tag="PLF66_02045"
     CDS             48387..49712
                     /locus_tag="PLF66_02045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62845.1"
                     /translation="MIKPKINLSIILIYLFLSFMYSESFAGDLIPKFEKEDFQVENYN
                     FFNNNRFDAPGNENFFRLYFQENKLRVAMYRDISYNSADLEYDEEYVAYYSIWGELEF
                     AYFRYLKNKNVDDEYCFGTIKKNKTENYCDYRTEDKGVLIKKTQNIKIKIPSTVNKLQ
                     TLVLELKEKSKQRNLFSHITNINTQLRRPKNEEEVNIIHHKVPIKESPSIESKTLKIL
                     YPYQKVKLIEVITDKNEKWAKVFVTDNFKEENGEPLDGYVNLDFLSLIDESKDRFERS
                     ILENKDSYWVKNSCSRSIPYSIMEESNFFSNPKDGIFIESKDIGKDLEIQIEHKGCGE
                     YWLTVNYILKNKNIKMIPNDTFWINQYLNLLVLTDNKILISKESRGLPFEIKEVIKVI
                     RKKQIQKKRIIYGRNYRFSDNELGQYFRFYKPKTKLKGIFLKVDFIINL"
     gene            49844..50026
                     /locus_tag="PLF66_02050"
     CDS             49844..50026
                     /locus_tag="PLF66_02050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62846.1"
                     /translation="MTASGELQRLSFYQSPSHTQRSSSPILFTLSIPFLVVGGIIGFP
                     ALLAAHYEEPIYKFRR"
     gene            50165..50596
                     /locus_tag="PLF66_02055"
     CDS             50165..50596
                     /locus_tag="PLF66_02055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62847.1"
                     /translation="MNHPLKKIICMLLINIIHCKEKIDPHSVDKEEIIQAQSSSKMGQ
                     RKLIEGQSFAVELEDFGKVKFASYIDSEENRMQNLSFVLEDNGKEVYTFPFVMGDSGQ
                     HFFFYSIKAISFLDMDGDFKKDVTYIIKLFTRCRKMFRLCI"
     gene            complement(51132..51632)
                     /locus_tag="PLF66_02060"
     CDS             complement(51132..51632)
                     /locus_tag="PLF66_02060"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002726225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HMZ62848.1"
                     /translation="MRFIFRFFILLTIVSFLIPESRIMPVDGGNANSYNKKSFWFYPW
                     GISGTHKGVDIFAQKGTSIFASTNGLILYAGEIELGGNAVFLIGRKLHIHYYAHLKEI
                     KTSSFSFVRKGTLIGSVGTTGNAKGKPPHLHYAIVTLIPYWWRIDSQREGWKKIFYLN
                     PIDFLP"
     gene            complement(51950..52633)
                     /locus_tag="PLF66_02065"
     CDS             complement(51950..52633)
                     /locus_tag="PLF66_02065"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HMZ62849.1"
                     /translation="MLLIKFIPSGTIFIISFLFLFLNCHILVIDIENKRYYPQPDKEI
                     SQKYGTLIWGKFEASSPREVSCQNGIKPIVIKRTIFDFIVHYLVGGIYTARSIEAECN
                     KEESFEIGKSLTENKKLSLNSIYFNLNSAEILSESYPILNQVANALKKENFTKLTLLG
                     HTDLTGNSENNLILSQRRAESVKKYLIEKGVAQNKIFAKGLGSTKPLVNLSDEEASKK
                     NRRIEFVLE"
     gene            complement(52624..52992)
                     /locus_tag="PLF66_02070"
     CDS             complement(52624..52992)
                     /locus_tag="PLF66_02070"
                     /inference="COORDINATES: protein motif:HMM:NF018045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62850.1"
                     /translation="MKQSKLVVFLSLFFHLIHCQHARLRVAPPLPKSCQMENHKQCEK
                     DKHEFLEKIKTSEKTTKEIHQTYFLGGLLPREYKIQESKYCPDSGIYEILQYYTFEDA
                     LFEQLTAFIYSPRTVRITCY"
     gene            complement(52994..54025)
                     /locus_tag="PLF66_02075"
     CDS             complement(52994..54025)
                     /locus_tag="PLF66_02075"
                     /inference="COORDINATES: protein motif:HMM:NF019224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1554 domain-containing protein"
                     /protein_id="HMZ62851.1"
                     /translation="MKNTKKHLILFILLLYCNCTGWPLLQHEVATSLQTGDNKTNSIY
                     SLLFIRPNQNSRGNSGVTTTSETNTPTSLASTKDILSFGIQTPNATGAISGTNITVTV
                     AGNTNLKNLIANFTHNGSSVKIGAVTQTSGVTSNNFSYTMTYTVYAQDGSSQNYTVNV
                     YATSCSAAAFCRIFAHAPASPAADFGSIPGAFGDGIVGIDTECNNGAIMIGLPPSNYR
                     AILMTTGGTTIRNQTTNWVLKPNKQYRRQDGTTIIGTTNASSVFAVSGGNLTNSVVGA
                     FSNIYTGILVNSDTSWTPSASNCSNWTSSAAVSVYGANGNFTNTLSFSDAGNSQTCNT
                     GVGVYCVEQ"
     gene            complement(54470..55183)
                     /locus_tag="PLF66_02080"
     CDS             complement(54470..55183)
                     /locus_tag="PLF66_02080"
                     /inference="COORDINATES: protein motif:HMM:NF021507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HMZ62852.1"
                     /translation="MRIKTKLIIIFIFLLVSGTYYLHEFNYPEIVYTEIDPNLVDIKF
                     FSKDENGNHFRNIGSLKNHLNNTLIFAMNGGIFKEDLNPLGLYIESGKMETAINLKSG
                     YGNFYMNPNGVFYLTKNKKANIVPSSTFTYDETIEYATQSGPMLIINKKIHKGFHQDS
                     KNYHIRNGVCILDSGKLIFAISKNKINLYQFAKFFEDRECLNALYLDGFVSKAFIPEK
                     NLEELEGDFSIIIAAIKEK"
     gene            complement(55490..56410)
                     /locus_tag="PLF66_02085"
     CDS             complement(55490..56410)
                     /locus_tag="PLF66_02085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62853.1"
                     /translation="MFLFTFIFSFAFFLNSNLYSQHMENKYIKWDLNHLYLSLPDTLI
                     AEHSIFFGTKDSNETEDEKGEIHIEFEDFPESVTPEIFTKTTMKPFTNSPEIKLEETK
                     NFKGSNFEFYTLMISFPKSKDEFFYFASFRLNSSIGKIRYTPKKRNDKLWNQILNTLS
                     RQRDVIIPKSGNKILCGGEICLELPNYYEDYKTHEFYDKHYKMDSKIILSISREEFKK
                     DYKDVYGYGDQYKAKIIPILKKKDTLPNESQVELQLLENNKLIKKIWVSRAKVKWKNG
                     KFISIQLQEEGNPDFSKNHWKPILQSIELK"
     gene            56809..57237
                     /locus_tag="PLF66_02090"
     CDS             56809..57237
                     /locus_tag="PLF66_02090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62854.1"
                     /translation="MKEKKSYENKVRVEERCYLVQNFERLVITLDVADYIHCPKIYNQ
                     LYDVQEILSKNYKAMLFIQEKIAIVSQKCPHKNSATENFNNSVKENLDFLSANVPNYV
                     EKFANRLPDEMEKIAANPTHYPLEPLLDSEQQEYRYSKFL"
     gene            complement(57347..57931)
                     /locus_tag="PLF66_02095"
     CDS             complement(57347..57931)
                     /locus_tag="PLF66_02095"
                     /inference="COORDINATES: protein motif:HMM:NF017496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HMZ62855.1"
                     /translation="MSAILDLPSIKSRTHLISIREYHALGEMGFLSKRTELIEGVVIQ
                     KIPKSPKHATMILRIMNFFSDKISKKLHLRSEQPLTLTRSEPEQNISIISGNLEDYSE
                     SHPTTAELVIEVSLSTLAEDREMANIYAEAKIPEYWLFNLNNNTVEVYQKPKEERYSE
                     IKTYSETETIVPEFDTSIKLELEAMFQVSSSNKK"
     gene            58057..58629
                     /locus_tag="PLF66_02100"
     CDS             58057..58629
                     /locus_tag="PLF66_02100"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HMZ62856.1"
                     /translation="MDYQQKGFEKLKQGLGTSLVFLAILWGIVISDFILPMDFTTLGV
                     YPRSLFGLIGIPLAVFLHSGFSHLASNSLPLLVLMTTILIFYPKVAFPSMFFMVIYTN
                     ILVWIFGRSAYHVGASGLVYSLVAFLIAAGYYKKRPLFALVALVIGFLYGGLVWGVFP
                     GFVGWYVSWESHLFGAIVGVYLAHFYKKDL"
     gene            complement(58649..59050)
                     /locus_tag="PLF66_02105"
     CDS             complement(58649..59050)
                     /locus_tag="PLF66_02105"
                     /inference="COORDINATES: protein motif:HMM:NF018738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein"
                     /protein_id="HMZ62857.1"
                     /translation="MGFIDSLKSMVGSGSPTVEVKLFKNKASMQESVKGIATFTGGEY
                     PVTIEKIILYILTVEELKDQNKTKESDEKVGIVSLNDYVLEPKEVITVPFQLKIPKDN
                     LITSSAIKHYIKVLLDISGQDVWGVQEITVV"
     gene            complement(59061..60887)
                     /locus_tag="PLF66_02110"
     CDS             complement(59061..60887)
                     /locus_tag="PLF66_02110"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HMZ62858.1"
                     /translation="MHYETQSNESFFTFEIEVKANLGKVWQYVSDTDRANQFLSEPAM
                     AYRDFPLPQGGSKKQGSQKNTGFHFEFEEKSSEWVEGKFFQIVREYTKGPFIRFIHRL
                     ELKEIDREHTLIQTTMRVILRFKLLTPIMYLKFNFDVIPKFKNYYRQIEHSKENTYNI
                     GITEIKHNLEFHDLSPLLHKFATISHNDTLSNLVASFILKSQEIDLVKIRPYRLAAIW
                     HEDKKDVLDFFLRGTKEGFFDLNWDLLCPACRGAKSSYKTLAEMEKKVHCSSCNIDYN
                     ADFDKSVELTFTPNPSIRKVVSGIYCASGPGSTPHLKSQIRVAPNQTEEIEYSLLPGI
                     YKIYSLQNNYTLQIQVSETGDKIDTIHYTGAKNEEVKTSLGKKRILLTNPNPKEEITI
                     KLEHAVWLENIVTAAEVTAKQEFRELFSSQVLRPGEEIGIKNLSVLFTDLKGSTEFYN
                     TRGDAFAYKAVSSHFDILIRNISKFDGAIVKTIGDAIMGVFLLPVNAVSYALQIQREL
                     RELNKKEYGENVIVLKVGIHSGPVLAVNQNEKLDYFGKTVNLAARVEGKCKGGDIVIT
                     KVMYEDNDVKSLLVKEKIQTEIFASNLKGFEESSELVRLILD"
     gene            61068..62630
                     /locus_tag="PLF66_02115"
     CDS             61068..62630
                     /locus_tag="PLF66_02115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62859.1"
                     /translation="MKIKIIKMFSLLLIPVLFLMECKSKSNDDNKTNLLVLAALANRS
                     SGSAARDMYTGFADIPASIYKTVASGSASSSIQAESRTLYTADYRFQNSTNTASVSGV
                     YDLVRATAKTTRDIAKSIGDLVGSLETIPVTTTLTGTATWANVASKYRYAPSTILTNG
                     KKLEVWWNNAAAPYSNNKAIELNYTGSTASGNMSGFVFVRFLQTSTSTTLSKAYINFN
                     YNASTGTRVMVVILQDINGGGTGTNFRDRAHFYVQEVNGVTTMDGTYTITGFNPNSTG
                     VNASDRAYVFSAIGNSSRAVVNAAFPQVTDNTTAIYATTTLGNIGQVWTNFILANTSI
                     VSGLNTIGGSCSASNITTTSASGGNPGTLIATNSIANLKTCLDSINPAVNAKDVYFLT
                     NIKNPAYFSVSGNTVSLYGVESLDASDANKSAFDALQNATSFTPISSAVRTTSNTTYA
                     AAFDAITVYSLNLFTGTGVLAGTGVLVGSSATSLTSLNAQWGNGTPGTGTSSATAGTN
                     SVNGSSDNTAPF"
     gene            complement(62862..63389)
                     /locus_tag="PLF66_02120"
     CDS             complement(62862..63389)
                     /locus_tag="PLF66_02120"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012476548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HMZ62860.1"
                     /translation="MNIALIGPRGVGKSKVSRKLSKLLSMPIVSTDMIAVYEAGGISI
                     AEQIKKENGKWNNFRKLEFKILENLSKSRNIILDCGGGILFDVDRDGKEFYSEKKTNL
                     LKSFCTVIGLSNNIDYLVEKVINDTERPQLSEINSYREVLERRIPFYKAASDFYLPVD
                     GLEPKDIAKKISELI"
     gene            complement(63386..64237)
                     /locus_tag="PLF66_02125"
     CDS             complement(63386..64237)
                     /locus_tag="PLF66_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HMZ62861.1"
                     /translation="MKDQIDIILADINKLPPMSNVVMRVMTLIQDPAVSIHDLANEIS
                     KDPAITASIIKLSNSAYYRASKPIRTVQESLMTLGIKTVKEIILLTASKKILNKDLPG
                     YQLEAESMWLHSLVVAELSARIAIQKKTGIPKDLAFTSGLLHDIGKVILAQFFPSKIL
                     EIKQELKTSKLSFTEIERKYFGYDHQEVGMKALQTWNFPEELKEVVAYHHNPDQAKNF
                     QKLVSVVHVANQIAVISGIGIDIGGISHELSPNAIKILGITEGDIESYYITIPELQKS
                     IVDLQAI"
     gene            complement(64242..64748)
                     /locus_tag="PLF66_02130"
     CDS             complement(64242..64748)
                     /locus_tag="PLF66_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HMZ62862.1"
                     /translation="MLSKDSKIINVGIAEIAVGKSPNILRTTLGSCIGVVLYQPDLKI
                     GAISHIMLAKDTMGRDKLKNPGKYGETALPKLIKQFEDEGCKPGTYYARIFGAASMFK
                     NITSAFLNNIGESNIAIVREFFAEKKIPIIAEDVGGHEGRTISLYLDDGRILLKKAGI
                     EKYLYKVR"
     gene            64887..66077
                     /locus_tag="PLF66_02135"
     CDS             64887..66077
                     /locus_tag="PLF66_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004757417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HMZ62863.1"
                     /translation="MDTIKEEAISKEPVRKIKINQSEVTILGTAHISVKSVEAVRNII
                     DQEKPDVVCVELCKSRMTSIQDPEHWKKLDIFKVFKERKMYLLLASLILSSFQKKLGQ
                     GEIKPGDELKAAIDEGNKIGAKVVPVDREIQTTLKRSWGNVGLFSKMYLLSALITSLL
                     VKEEVSPEKIEEMKSDDALKDLFSQLPPRYNSIKEVIIDERDKYLAENIRRQARDSKK
                     VFAVVGAGHLEGIMKHIVEDQTIDHLDEIPKSGIWDKLQLAILPLFILSLMAATFYFS
                     GFEAGTELVKEWIIVKGTLAALGALIALAHPISILLAFIASPIGNFNPIIKPGWLAAL
                     TEIWMKKPLVEDFEKIATDSEHFTGFWKNRVLKIFLVLLLPQIGSSIGTFLVTWKGIK
                     GFLG"
     gene            66085..66765
                     /locus_tag="PLF66_02140"
     CDS             66085..66765
                     /locus_tag="PLF66_02140"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HMZ62864.1"
                     /translation="MLKLATSEQRDFYEKKLYPMQDKIFSLLQSDKFYLTGGTCLSRF
                     YYNHRYSDDLDFFFMGTDFRREDFEAEFREIQKRIHLSFNSKLEISSDYFKRLTVFEN
                     EQTLKVEFIYENFQMVQKPIRNDLIFLDTKENIFGNKLTAIHGRKTTKDYFDLFFLLK
                     ELKLSDGIKWSEFKHAPVDYEGAVLSLVGGQLEGDVFMMQEIKLDDFNEFQNNLITEL
                     LSYAKTVS"
     gene            66746..67039
                     /locus_tag="PLF66_02145"
     CDS             66746..67039
                     /locus_tag="PLF66_02145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62865.1"
                     /translation="MQKRFHKYFWDGLENWSPEFQLRRIIEYASFPDLIQYPFEEVKE
                     NLSKIRLEKLRTGENRKELIIKLSPFVSQASSWEEAIWKMIEYYREQNKERIA"
     gene            67041..67610
                     /locus_tag="PLF66_02150"
     CDS             67041..67610
                     /locus_tag="PLF66_02150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62866.1"
                     /translation="MLDFVDKFKSELLGIQNTLNELLGIKTFIDSLKKTAQFFDLFFS
                     IVPFEVILLLIFCALFLVLVNNISPTTPRINITIAVGLFCVISLYINHIFTGEYKFLH
                     VFYISLYVLVPAYSVELSKFAYKQIYNIRQKSMKIENPEELVKSIREINQRYYEFLNA
                     QADFKNNPLDLKKSAANLKSSIEQLEKRI"
     gene            67607..69130
                     /locus_tag="PLF66_02155"
     CDS             67607..69130
                     /locus_tag="PLF66_02155"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HMZ62867.1"
                     /translation="MSRRRYILYALAYITIPVLTNMVIAAFSYTVLVSFFMTREEFQV
                     VSQVGESGDVTSLIFSYASFVIPTAIGYAYNLPLIFYMFKKERPPMSIKIQSIIVNLP
                     IVNATVSFAGWGLALCGTFTYFYNYDVYISLQASLKLICFNILMANLCFVFVYYLVDI
                     VNKRFILLVLKGQNLSSIPKTVKISIRGRFFIFFLTVAVTPCLLLIGLILSILSENQI
                     SRDYTHVGYIFIGIILTGVILTLFISNSFANPILDLQKATEKLQSGDLDSRVSVYTND
                     EIGFLGENFNHMSSSIKEKEFIKDTFGKLVDPTVRDFLLEGNMTLGGETKDVTVLFVD
                     IRGFTSISEKLPPKLIVNWLNQYFEQMSNSISKEKGLVNKFIGDAILAVFGTPIPLEN
                     HADSALRAALDMRNQVKLLSEKFSKEGLPEIKIGIGIHSGKVLAGNIGSMNRLEYTVI
                     GDTVNLASRLESYCKTANRDLLLTEATKELIQGNFSFEFIDKIKVRGRESEISIFSA"
     gene            69176..71377
                     /locus_tag="PLF66_02160"
     CDS             69176..71377
                     /locus_tag="PLF66_02160"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HMZ62868.1"
                     /translation="MKDKKKILWFFILTLIPLLLMLLFRFSGITERAENMLIDFRHQY
                     FNPNHKFSDKIILIDFDEDTFKKLGNKKEFGRWPWKRSAYTPILEYLSLGAPKMVLFD
                     IMFFERSEESQDIALGDISDRLTQMGIPVSHAASFRYEEKTNAKPEDIPEDMRAALDT
                     ISMKFPSESDSILRKYNIFSYPTDDIMRKIRHLHSVSYKSDVDGVSRRIEPFFKYEKL
                     HLPALPFKGFDAVKPMDQIDISEKSLNLRSGDENTNIPLENGRMRLHYYSPEQMENIP
                     RFPVWSLIESYEKVKKGEADPANLETNFFDFQNRIIIIGTSAAATFDDKVTPYGKMPG
                     FVLHAVVLSNLLEKHFLKLSPLWFNVLLLVITIPTCVFFSFFFQQMALRVFVPAGIFL
                     GYTLLALFAFKFDIHLPLASFLTAYPLAFLGSLGYLSFTEGAERRKYSKVLSNMVDPS
                     IVSEALNDLEALKKGGEREITAFFSDVAGFSTISEQLSSADLAALLNEYLSAMTIILK
                     HNHGTLDKYIGDAVVGIFGAPIHRDEHFIEAAKASLEMIEKLKELKAYWSANNLYCKD
                     AQDMDVRIGLNTGLAKVGFMGTDALASYTMMGDTVNLAARLEAAGKDYGVNILISEFT
                     NDKIKEQMFTRKLDRVRVKGKNEPVQLFELIAIKGQESESRRESAQIYEEAFGLYLKQ
                     EWDKAIELFEKSQKARGSKDKAAEMLMDRCIYYKNSPPGVEWDGVFTRKHK"
     gene            71648..73744
                     /locus_tag="PLF66_02165"
     CDS             71648..73744
                     /locus_tag="PLF66_02165"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62869.1"
                     /translation="MKRKILLFTLITVCVDFLSCNGTADGKSSVGQLVLLAFRAARLA
                     NERVELSGTVKGPGSVKNASVKVFPTPANGQCIKEDGTLNGTPIATTKSDENGKYNLS
                     YKKTGTTVCVVVVPDDGSQIDVFSPASRTNAPKPWSGANNLMAVVNEPSGRANSIGAS
                     KTVNVTPFTRMAARRFGALSTIAQNNGGKVFLGIVPVGRRAPGDDKRVEFGNNNATTI
                     IDKANEDVENAFFPKQNKTGGFSIESTDVNDPSYALKLGSILITADKLGGGVVNGSTT
                     ASDLEKVINFMEEDFSDGRFDGKKIDSNTGKIAPLTSSDLGGVVANAKAADNFLKVDF
                     KAAQQEYDSYDSSVSSTSDDLFFCETDTLDAACNIAVLPGSPPEIWIFNQDEDFIDIG
                     DIYDHGSVGFGTGGTASTRYYAIVNGGGSDLKLTLPFTFSGSQFTVVEQPDAVVAMGD
                     ATYFAVKFVPSSATTFSNTMTVNSNDTVYSPYTVTLTGEGVDLGTNLQLYWKFSGNTN
                     DSSGQNRIGTKVGRTVLASLTYDYWDNIDSAFNFDGTSDEITNTFSFVLGSTGTFSAW
                     VSPADLSNPSYSILKRGTTPEFQLMFLDDVTLRFTVTTSVGGTLDLDIDIDPDDFIDQ
                     WNLVTGVYDGSNMRVYINGEEYDFQAKTGTLTNSTGFSVGNDFFGNFFDGDIDDVRVY
                     NRALTPAQVYALYNQD"
     gene            complement(73741..74517)
                     /locus_tag="PLF66_02170"
     CDS             complement(73741..74517)
                     /locus_tag="PLF66_02170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62870.1"
                     /translation="MTLIDETISEEVKVKLDKVKSIHPFQLLFYGSKSRGDDNELSDY
                     NFYLLANPTDQLRTSFIQEISDALEYIENGSAVSLVAGDLDSLTFRMKIFEPTAVHLC
                     ELAQVAYGKNQLESLRKEWELIRSKEIDRKVILNYLDNRCKFYKSLKSKNTKEDISRI
                     EKVISLNLQMWIFANIEDLTPTEVCHMDIPARLYAMLKYLYKNEATDQVNILIEIYKE
                     IHELKQTIRMTLPYSEDSLSRIKDSIQMIQGLSNNIVNKT"
     gene            complement(74519..74725)
                     /locus_tag="PLF66_02175"
     CDS             complement(74519..74725)
                     /locus_tag="PLF66_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020762606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62871.1"
                     /translation="MWLKLGETEVINLDFVSTIKKNANNPTIEIIYHDLNNIKSLPFK
                     GNDERDRAFKAIIENLARMKLFFE"
     gene            complement(74851..75582)
                     /locus_tag="PLF66_02180"
     CDS             complement(74851..75582)
                     /locus_tag="PLF66_02180"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone/menaquinone biosynthesis
                     methyltransferase"
                     /protein_id="HMZ62872.1"
                     /translation="MSNFKMPEANVKAEFVKSNFDVIASKYDLFNDLNSFFMHRQWKN
                     SIIRKIEQTFPYQKLTCMDLCCGTGDISLRIAELENTKELFSVDFSENMLNIAKQRLT
                     GIPKAKQEVGDATQLKNFANASFDVVTIGFGLRNVDNLEKALSEIYRILKPGGLFINL
                     DVGKVKNPIIRVFADFYFFKIVPLLGYLIWGSKNEMFDYLPVSSLHYPDQENLKKLLE
                     ENKFQNVEYKNFVFGNVAMHWCIKA"
     gene            75668..76528
                     /locus_tag="PLF66_02185"
     CDS             75668..76528
                     /locus_tag="PLF66_02185"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HMZ62873.1"
                     /translation="MESLTTYFVPVFAIFILVENLIAFKKNPERFKIKDSFTSIIMGI
                     GSVLINLVTAGIVYKEYSFVSQYAFFKIDPTWWSWVVLLFAEDFCYYWFHRVGHESRF
                     FWASHVVHHSSESYNLSTAVRQTWTGTMINAVFWFPLLLLGFPPLMVMMQQSISLIYQ
                     FFIHTEQVRKLGPLELILNTPSHHRVHHGSDLKYLDRNYAGIFIIWDKMFGSFQGEEE
                     VPTYGLVKNIHTYNPIKVAYIEWWNMFKQMFQVGSFGNAIKVMFKPPGWSPEGGTTTA
                     ELQKQAGLRS"
     gene            complement(76555..78948)
                     /locus_tag="PLF66_02190"
     CDS             complement(76555..78948)
                     /locus_tag="PLF66_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016559522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HMZ62874.1"
                     /translation="MRLIKIILFLTISFIITISIQSQTFGPKTSGTGLKPDSYDLLSS
                     IKEANPNRIAHRGLPSKVDLSDLMPPVGNQGQQSSCVAWSTAYANKSYQEFIERKDKG
                     TWNYKTGNTPNYKNLFSPAFIYNQINGGRDNGSSISDAMALIVSKGVLPWDAMPYNEK
                     NFSKQPTAEHLSLAAKFKAKEFLRLRYNEPSEIKNHLSQGRPVVVGILINENFYEIGK
                     KIYNEGKGANLGGHAITLVGYDDSTNAFKFQNSWGVEWGDKGFGYIDYKYFAKVCRSA
                     FVMIDLIDPNPEPQIVDNKIKPTPPENTVLPPTDINPPSEINASNGNFSNKIVLTWMQ
                     VPGAIGYEIYRSNPDDDNFQQIGLSQNTQFEDTGVLPEIAYSYKITAVSETDVSDLSQ
                     VTAIGYAKDTKNEIPPKITSITATDGKFSDKIILEWEPLENVTGYQIFKWDSATKTYR
                     AIAKTNSTIYEDRTAKKNGTLETYTVAGLNNSITGILSDAVMGKTSLIAKPQAPDRVV
                     ASMGQFRDKIVVKWNKVSGATGYLVYKFVDNKWETLGETAEDQLEDAPATRGQRYYTV
                     IAKNKDNIWGPFSRYAVGYVDPNLRRGGSKLEPPSGVSATVDKKNGFATITWHKVDGA
                     EEYNIWEKKQGESKWNFKSRVDSNKLFYTFTIPEREKFYLYSVTSKTQLGTDSDYSNV
                     ASVVLSTAKIASKTRSFGGASKFEKFTGTWTAMQWDGNVGTKNVIMEITQTDGNNFTV
                     KIDNKKTFKGSYVQGSPIIDIDGKIKIKLASSEDALMVEMKDKSIVNEKTELSFLKE"
     gene            complement(78968..79402)
                     /locus_tag="PLF66_02195"
     CDS             complement(78968..79402)
                     /locus_tag="PLF66_02195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62875.1"
                     /translation="MKIFLLSFFCILLYAEAAPTPPSFEELFKKSDYIAKTKLVNLKE
                     KQINKNLIAINTTAEVLKQYKAVSPMPEKIDLAFTVLPEVYGKWLKVPPKEGEYVIFY
                     INKEVKDGKGNRHTIISLYEPHVFAFREWTEELEKRLLESGK"
     gene            79650..80048
                     /locus_tag="PLF66_02200"
     CDS             79650..80048
                     /locus_tag="PLF66_02200"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HMZ62876.1"
                     /translation="MRYVKRKTHQVGNNSIDMSSLMDIIFILLIFVMMSVSFAKNFKQ
                     LEIDMPDSKTGNSELSPDIQISIKANDKYFLDSKEISFSELSSIGSKGQFNNKTVSLN
                     VEKKVPYEKFIQMIDILKLGNTKKINLGTE"
     gene            80167..80658
                     /locus_tag="PLF66_02205"
     CDS             80167..80658
                     /locus_tag="PLF66_02205"
                     /inference="COORDINATES: protein motif:HMM:NF016854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HMZ62877.1"
                     /translation="MDSFLDFALERLSIAGQVTAKPMFGGHGIYLEDLIIGMVVESTL
                     YLKVDDSNIKDYIQAGVKPFSYEAKNGKPISMSYWCLPADILESNEDLPKWLNKAVEA
                     ARASKKNKTKKHARVKSKVAAIQKKKQAKTKTPASKKQSKKKPAPAKKKAKKKATAKK
                     KGK"
     gene            complement(80675..>82017)
                     /locus_tag="PLF66_02210"
     CDS             complement(80675..>82017)
                     /locus_tag="PLF66_02210"
                     /inference="COORDINATES: protein motif:HMM:NF023459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="von Willebrand factor type A domain-containing
                     protein"
                     /protein_id="HMZ62878.1"
                     /translation="DTASYSNTRRFLNNGSLPPKDAVRIEELINYFKYNYSEPSGKEP
                     FSVGNEISYAPWNEKNYLMKISIKGKSIERKEMPPRNLVFLLDVSGSMLTADKLPLVK
                     QGLKMLLGELTEKDKIAIVVYAGASGLVLPATSASQKDKIINALDALEAGGSTNGGAG
                     IQLAYKIARENFNKQGINRVILATDGDFNVGIASQGDLIRLIEKERESGVFLTVLGFG
                     TGNIKDSTMENLADKGNGNYAYIDSLQEAKKVLVTQSGGTLVTIAKDVKLQLEFNPKF
                     VKSYRLIGYENRVLRNEDFNDDKKDAGDIGAGHNVTALYELIPIDGESNTGSVDPLKY
                     QEERKRTKSADLNEILTIKIRYKEPDSAKSSLLTVPIQNKILDLGQTSTDYRFAASVA
                     SFGMLLRDSEFKGKANWKDTYDLAQKSIGSDKEGYRKEFLKLIEQARNLSEKRK"
BASE COUNT        26563 a        13104 c        14594 g        27756 t
ORIGIN      
        1 atgtctcatc tgacaatatt ccttcggact ttccgatccc cggttgctgc cggggtattt
       61 ttaataagcg tcctcttgta ctaaacgctt gtttatgtca cattatgtgt actattttta
      121 aaagtcaagt acaagaactg gctgttcttt ttttgctggt acatggtttg tgtatggatc
      181 aatttttact gcccttctca caaggtaaga aaaagccaaa tctgtttata aatgctcgat
      241 ttacgtttta tatgggacgg ttttacaaag tcttaaggta aatatttttt aagaatactt
      301 ttaactttcg attcaatttt gagtagaagt tttgggtcat ctattgatat aactaattta
      361 ttgtcgaatt tctcaatgtt tacatgctca ttagactttt tattcttatt ggattttgga
      421 ctcgtaagga tttcgctgtt tttgttaaat tcctttgctt ctttgattgt tcgaaaggtt
      481 ccgtttttat aactttgtat aaatttatta aaatcacctt ttttgtagga ttgagctgct
      541 tggactagta agtttttatt gtcaatacct gcatttcggc atatctctag ttcttttgtg
      601 cctagatttg atatgctgag tatttctgag atataatttc tgcttttccc gaaaataatt
      661 ccaatttcat tgtccgtata gccatactcg gattttaata ctttcatcgc ttctgcttca
      721 tcataggcag ataaattttc cctctgtagg ttttctataa cggctagttt atatacttcc
      781 ttttcgggct tattgagaat tttgcattcc acctcggtcc atccaagaag tgatacagca
      841 tgaaaacgtc tttccccggc gattatttta tattgttcct cggagtcaga ttttgttaca
      901 acgataggtt gtaagagacc atctgccatt atactgtttg ctagttcctc tacgcttttt
      961 tttctttccc ctctgggttg tttttctgat ggctggattt ttgaaagttt tatctttcga
     1021 atggtccctt ctaggacttc tgcctgaaaa atatcagcaa gagagcctaa tcttttactt
     1081 tttaagctca tctaaaaact ccttcacaaa gtcttcatat tctttagctt gtctacattt
     1141 tttgttgtaa tcatagatgg aacaccttgc caaatgactt tcgccaatgg caactccatc
     1201 cgagatgcag ctatcgaaga ctttgaaata ctttgtcaga attgggataa ttgtttttgt
     1261 gagcaaggta tgttgcttga gttgcgtaat gagagcgcca atgatctcta gttgtggatt
     1321 gatccttttc tttatacttg tgattgtttg ttgcaatccc acaatcccat ccacagagaa
     1381 tttttctgcc tggagaggaa ttaatacgta attagaagcc accaatgcat tgattgtaaa
     1441 aatagataaa ctcggcgggc agtctatgat gcagaagtca aatcctgtta atccctccat
     1501 tgagtctcgt aatataaaag gggcgtctac attatttgcg ctaactgctt ccatctcagc
     1561 tagtctttgt gttgtgggtg caataaataa gttattggtt ttagtttctt ttatgattga
     1621 tgctagtttt gctttattat taaaaacatg aaacatgttt ttctcttcat ctgcttttga
     1681 tgtatatatg ctagatgaat tgctttgagg gtctatatcg agtaaaattg tttttttccc
     1741 tctccttgca agcccatcag ctaaatttat ggaagttgtg gttttcccct ctccaccttt
     1801 ttggtttgtt acggatattg taatcattgg atctgtgttt taacaggatt tgtattcaat
     1861 attagatttt caattagaaa acgatcgtaa gacttgattt tgtgaaataa tagaaaaccc
     1921 tttttagaga acctaatctc aattgattcc ttccttacgg aaatactttt aatctaagtt
     1981 taatcttgcc ttccattcaa attgaaaaga gtatggttat gaagtgtaga agtttattta
     2041 atttttaaat tcacagaagg aaacaactgt cggacgtccg accgatgttt aagatgggga
     2101 atgggagaaa aagaatcaaa tatgatttcg ggtttaataa aaaaatcaat gcaggatccc
     2161 aaaagtttct atgaaaatgt tgaagctttt tttgaagggt ttgctcaaat tctagaatgc
     2221 aattgtggaa tattggttct ccaagataaa gaggattcat ttttagaagt atcctctttt
     2281 ggatatttag atgattggta ttactccttc ttggcaaggg ggttaggtaa tttggagaaa
     2341 atcatttcta cggataattt tgtctacttt ccttctgaga attttaaatt gtacaatcct
     2401 aaatcaaaac acggatttgg atatagaatt gaatgtcaag gtaaggtttt tggttttatc
     2461 tttttggaat ctgttcaaga gctttctgat ctaaagaagg aatttgtaaa aatcatttgc
     2521 aatgatattg gatttataca tttatcaaag accaatttta cagaaaaaaa accattggat
     2581 cgagacgaga tcagtcatga tctttttgca tttctattga gggactcgct tggatttact
     2641 aatactttaa aagcaaatcg atttataaat atacaaggcg ggagaggagt tggtaagaag
     2701 tctttggtga aatttatcta taataagtta ggcttacatg gaaagttaat ttatttaaat
     2761 gccttgcccg actctgttga gaagttagaa aaatcaatcc gagattggat attgcttgct
     2821 gagaatggga ttttgatcat tgaaaatgtt tcttctttgt caatttcgca acaaagagtt
     2881 ttttttgaga tcattaaaga gccgatccta gagccaagga ttattttctt ggatgatatg
     2941 atgaagcatc gagaggaata tcaaccattt tggaatctat tgtcgcaaca tacgataagg
     3001 cttcctgagt tattaagttt agataaagaa gtttttagag aaattgtaaa tatacttttc
     3061 tatgatttga gacaacttca ttctaggcct gatatgatgc taatggatga ggtttatgaa
     3121 tttctttttc agtatcccta taatgagaat ttgcgtgagt tgaggaatct tttagagatg
     3181 ccacttatca acttaaaaga aaatattatt acaaaagaat tttacttgaa attaaataac
     3241 tctgaaataa aaagcttaga tttttataat aaagaagatc tagatttaag aaaaagtgta
     3301 caagccttgg agcggcaaaa gattttattg gcaaataaac tcttttcagg caaccaaatt
     3361 aagatggcta aagcacttgg agtttctagg gggtctttgc aatataaaat gaagcaattg
     3421 gagttacatt gatgttatcg aaccaagaga ctgttgaaga gagaaaaaca ccagatggat
     3481 ttgtggtacg agttcataga gctactatga cctatgaagt attttcacga aagggagtag
     3541 aaattcctag aaactctaaa aataatgtat ccattatcaa agtgcctagg attattttta
     3601 tgggcactgc ttttaattta tcgcatttta ggttggctga tttgacttat gtaaatgcaa
     3661 gatcaggtaa actccaaatt ccatatcaat tctcagatag tagtgatatt cgaattctct
     3721 cggttccaac tggatcggat tctgatgtat tgccaattat tccataccaa tacaattcag
     3781 tgaaagaaat agttcatgca gttgaccaag gtttagagtt tcaaacaatt atagtagatg
     3841 aatcttctcc gaaaaatgaa atggtgaatc ttaagattcg atttccaaat gtaaatgttc
     3901 ttatactacg aaagaaaaat taaatattaa aaaattattt taattttgga agcctttcaa
     3961 ataatcgttt tacgcacttg tgaatttgga gttatttgaa tggaagagga tcaacaatac
     4021 gaagaacgta agaccccagg cgggttctta gtaaaagttc gtttagctaa gctcacatat
     4081 gtcgtattta cacctaaagg cccagaagtg cccaaaggtg caaaaaacaa ttcaattata
     4141 gttcctgtac cgcgagttgt ctttttagga gacgacttta atttatccca ttttagatta
     4201 ggggaactca gttttgtaaa cgttaaatct ggcaaacttg ttattcctta tcaacatggt
     4261 ggttctcgtg atataaaaac gatttattta aactctggaa gtgagagtga tattctaccg
     4321 gttattattt atcattttaa ttttgtaaaa gatatcatga gtgcgacaga gcaggggact
     4381 gaatttcatc acattgtcgc cgatgaaagc ttattgaaaa atgatatggt tacacttaag
     4441 attcgttatc caactgtaaa catattgatc ctaaagaaga aaatcaacgt tgcatacgaa
     4501 aataaagttc ctgcaaatgc aacaacagct gagaataagt ctgaatttgc aactcctgta
     4561 gtagatgcag atattgcaag agcagctgat atggcttcct ctggctccaa tttgaacatg
     4621 tattcggaga atcctgtttt tctggcaagg attcacctta gaaacttgga attggataaa
     4681 gtgaaacaac ttctgtttga ttttaactta aaaagtgaag atataatctt tattcgaact
     4741 ttcttagatg tcatgatcaa gaatgtgaat aataaagaag aactggttac aaataaagat
     4801 aaggtgaaag tgctagatga gatttttaga ctagcaatct tgataattaa tcataacgaa
     4861 gccgaatttg aacttgaact ggaaaagggc ttatcgaaag atgttgcata tatgatatac
     4921 gcgctcttag agaaatatca ggagcaagcc aagaacattg aagaagaaat tatgctctgg
     4981 gaatggaaat atagggctag aaggttaatg taatattatg agtaaagtag catttagtaa
     5041 attgctagag gcaatgaaaa gtttaaataa tgaagtaaac gaccaagaga tttgtagacg
     5101 tttggagact cttatgctga ccagcaaaga tgatctcaat ctatccgtcg taaaccatct
     5161 attggataat cccttagaat ttgatcctaa agaaatacca gagccttata caatgtatgt
     5221 aaaacacttt atatatatgg tcaaaagaaa tgaaaaacta ggtattccaa ctaaattttc
     5281 ttccccaagt tctgaaaagg gtggtaaaaa aaaagagtaa tctaaatttc gaagtcggag
     5341 gaagtcttaa aaccttttgg atgatagttc aatatagaaa tatccttttc ccaaagattg
     5401 gctttatccg cggagtaaat aaagactttc attgcagcat aggaatcatc taatgcttgg
     5461 tgaaaatctg ttcgatctga agtctttatc gtatcaattt taaaatattt tcttaaatgt
     5521 gccagtccat gcttcggata attcgggaat aaagaccgag tcatatttaa tgtacataac
     5581 actggtaggt tagggaaggg aatctccaat ttcttggatt cgaataatag gaaagacaaa
     5641 tcaaagtctg cattttgaat tactaacatt gaattttcta taaaatggaa aaattcggct
     5701 aattttttat ctagagatgg tgcattttcg agcatttctt tagtgattcc attgaccaat
     5761 tgggcatctt ttttaagttc ttttcgagga gaaataaaag aatggaactc tgccaagggt
     5821 ttatctttat taaatcggat agccgcaatt tcaagaattt cattttgtcc cggttttaat
     5881 cccgttgttt ctagatcaat tgctgtaaat tcataatctt tgagaatcat taggaacaga
     5941 aagctatctt atgccttcct tagcaattac tttatagata attgattgaa aacaagatca
     6001 taaagaaagt atccattttt tttaaggata atcctgtgca aagaggggtc catttcttca
     6061 ttttttccct actggtccat ttttaacgaa ttttgacgta aaatagtaaa aatctagaat
     6121 aagtgtcaca tttttggcat ttaaaatccc gtttttgata aaattagatc acgcaatagg
     6181 caaactgtca ttcagtcagg gagatttcgt ggtatccata tgtcagaatg gcagtaaaat
     6241 tattaaatat tgttaaaaat ttattgccca gccaactaag tcgtgcaaac tgatcgaatg
     6301 gctgaaacat taaaggtaag agtgcgctgt catgcatgca cctatatgat tgagggaact
     6361 gcaaaatatg gcaaaggtca ttacgtcccg gagggagttg actttgaatt tattgcaatc
     6421 gggaaggttg aaacatctaa gggaaggacg gtaaaggcgg aagtactttg tatttgccca
     6481 aattgcggag ttaaatgtaa gttctctgtc taaaaatagt caaaaactgc ttaaatttaa
     6541 taaatgatct ttacatctcc tctttttctg tttttctttt gctttgtttt cgtttttaga
     6601 tggatattta atcaaaaatt cacttctatc tccttgcttt tcttactttt aagcagttat
     6661 cttttttatg caggttggaa cttctacttt ttagggttga tttggttaac aacaattgtg
     6721 gatttttcta tcgccaaacg aatcgagttt agttctacca ggaaaacgaa gtttttttta
     6781 ctctttatat ccctgttttt taatctctcc attttaggtt ttttcaaata ttctgccttt
     6841 tttttagagt catttttctc tttttctgct ttaattggat acagcctgga tagaattgta
     6901 tttaacataa tccttccagc agggatttct ttttatacat ttcaatctct cagttataca
     6961 atcgatgtct atcgcggagt tatatcagca gaaaaaagct ttcttcagta tgccctttat
     7021 ctggcatttt ttcctcaatt ggttgccggt ccaatcgtcc ttgcacaaga ttttttgcct
     7081 caaattaaaa ctgtttttaa gaaaaatttt gagagtatta atctcaaaga aggaatgtat
     7141 tacatactcc ttggattttt aaaaaaatct gtcatcgcag ataatatatc tataataacc
     7201 gatttcattt tttcggaaaa taccaattta tcgtccattt cttggcagta tttactgata
     7261 ggaatactct cctactcgat tcaaatttat ggcgacttta gtggctatac tgatatagcg
     7321 cgtggtgtcg ctctgcttct aggctttcat ttgcctgaga atttcaatct tccttacttt
     7381 gcatctagct taacagattt ttggagaagg tggcatatct ctttatcaat ctggcttcgc
     7441 tcatacttgt atattccttt aggaggaaac cgtttaggaa atctttttac ctatcgaaac
     7501 ttaatgataa caatgctact gggaggatta tggcatggtg caagttggaa ttttgttata
     7561 tggggaggtt tgcatggctt ttatttagcc attgagagat tttttcaatc cagacctttt
     7621 attatgtccc ttgtatcgga tatccgacca tctctaaaaa cagcactctc tattttttat
     7681 tccattttta cattcatttt ggtgtcagtg ttttggatat tttttagatc ccaaactatt
     7741 tcaaaagttt tttcaattct ttgggggatt gttacattgc aatcaggaat cactccgagt
     7801 tattctgtat tcaatcagtt ttattttcta ttctttttag ttttaattgg acactcaata
     7861 ggatattttg cttctgatcg aattaaggac tttcttgcaa gaaaggatag ttattttatc
     7921 ttttcttttt atgcattttt attgattgtt tttgtcttat acagtggtga attgaaacct
     7981 tttatctatt ttgtttttta acctaaatca taagcataac tctttataga aaaataaact
     8041 ctgaattaat ttctttttag aatagctgag taggttgcca aaaaatgaaa ctattcatat
     8101 atgtaaaata atgagttaga aagtttacac tgaattttga tatttataaa cttagaagaa
     8161 tcaaaagaga aaagaatctt ttgtcatttg agtcggttta aaagcatagt aaaattcaat
     8221 ctagaatcat tggaaaatgg aatgcaggaa tctttgttgc cggaaactat tgataaaaat
     8281 ataaaaagct caaagaagaa ttggattcaa attttagtct ttgtcttatc tctcttttta
     8341 ttcgatcaga tcttttttgg gtttttcatt tggaatttgc caaatgaatc gccatgggga
     8401 acgaatcatt tttttaattt catctatgaa aaaaagagaa tcgaaaaaac gcaaaaagta
     8461 aatcagagga ttttaattat aggtagtagc attgcctatt actctttaga taaagattcg
     8521 attcgagaat atttgcataa aaagttaaac aaggattttg agatagaata tttatcttat
     8581 gcgggaatga ctccattgga ttcctatctg tttcgaaatc aaattgagtt actgaaaccc
     8641 gatttaattg tgtatccaat taattttata gattggagat tgcatcgagc ctatgtattg
     8701 aatccaaatg gtagcaatga aacagccgat gatcgaagtt tgattttaga tgcattgaat
     8761 tttggagatg ctcctcaatc aaaatttgca tttccaatgg aagcgattct tgaatttcat
     8821 tctcttcttt ctttggatag aattgcagat tacttatcga gttcaacttt cttgttctat
     8881 cgttatagag aaattttctc tcagaatcta aggaatattt acaatcatcg atttggtagg
     8941 aatattagtt atcatgggta tgcaggtgtc caaattccgg aacgtgtgaa ttcacttggt
     9001 tggaccggtc gcaatttttc tttttatccc aaggagtata tgttcgagag aggtttctta
     9061 atacaaattg ttccagagat tttagaaaaa ggtcctttga tcttgacact atcatctcag
     9121 ggattagaag aaaaatactc ttttcaggac acaggatgga agaaggttgt gcttcctttc
     9181 aaatttcaaa gtaaagaaaa taaaattaca gcttctcttt ccaatgtctg gtatcctttt
     9241 caagcaaaag aagatcgctt tgaccattct cgtgatgaag ttggagttcg tttgcagcag
     9301 acctttggat tagaccgccc attatctggc atgcaatata aaagagagga aaggattgaa
     9361 gacctaagat atatttccat gagtgatgag gaatacaaag aatattttga atatagactt
     9421 ctttctgatt ttgagcatcg tcctggtatt cgttatattc atgctttaaa aaatgcgaag
     9481 gagagattgg ctagcgaaaa gtttagacct gggattcata tgcaatattt gcagaaattt
     9541 cttgagtcaa tgaaacaaaa aaaaatacct attctgttga ttaacaatcc tgagaatccg
     9601 cttgctctgc gttggtatga ggattcacaa tggtataaag atcatttaga ttatttagta
     9661 aacctatctt cagaaaacga aattttcttt ttcgatctaa agcatagttt aaagatgcag
     9721 gattttagtg acttccatca tataactttt ccaggaatgg aaaagatgaa ctttatttac
     9781 ggtgatttga ttgcggaatt attcaaagaa aaataaacct gaaattagaa aaaaaacgaa
     9841 aactcaaaat gtgtctaacg tggggcaaat gagcgtttac cgctcggcat tgaaaaagtt
     9901 ttttttcaat aaaaaagttt tctatatttt cttatttctc tttttgcttt ttattccatg
     9961 ggtgcccgtt ttatattttg gggttctaat ttattttcct tatgatgaga ctggaaagag
    10021 taggtatatg cgtatcaccg gacctttagt tcaatatgtg ggtgcagagt gggtgagcac
    10081 agatcgaata ccaccggttt gtaaaaaagc tattgtcgtt gcagaagaca gcgagtttta
    10141 taatcacagt gggatttcga ttgaaagtat aaaatctagt tttgaataca atcggcgaca
    10201 ggggagaatc gtttctggtg ctagcactat cacacaacaa tttataaaga atgctttctt
    10261 gagtaggcag aaaacatatg ttcgaaagct aagagaggcg attggtgctc ttatgttcaa
    10321 tctccttttt tctaaagatc agcagatcac ttggtatttg aatgttgttg aattcggacc
    10381 taggatctat ggtttgcaat ccgcctctaa tttttatttc aaaaaagatc catttaaatt
    10441 aaatgctaaa gaatgtgcgg ttctaagcac cttcttaacg agacctgttc tctttggcaa
    10501 aagttataaa aatggtctaa cttctgtcgc attccaaaaa agattccacc gaatctataa
    10561 cggacttgtt cctcctccag ctaaaaaaga aaaacaacag ccagaagtca ttgaagagga
    10621 agatatcccg gaagaagatt cagagtaaaa aaggtttcaa atcaatctca aaataaagat
    10681 atccgaagag tgaacgggct attaggtgaa gttaattatc cctacctttc acgttagttt
    10741 tggaatcatt ttcaaaaatt tattgcttta ttgtttaagc atttaaaaaa atctatctct
    10801 aaggcggtat aaaatgagta aagttaaggt aggtgttcta ggggcaactg gctcggtagg
    10861 acaaagattt attcaattat tagaaaatca tgaatacttt gaagtagtag cgttaggcgc
    10921 ttcagataag agtgctggca atacttacaa agaagttatg aaatcacgat ggaaagtatc
    10981 ttctaaaatt cctgactatg ttagtaatat gaagatcagc cgctgcgtgc caaaagaaac
    11041 tccgaatgtc gatttggttt tttcaggatt agacagtgat gtagctggtg aagtggaaac
    11101 cgaatatgcg aaagctggtg ttatggtgat ttccaattcc aagaatcacc gttatgataa
    11161 agatgttcca atcctttctg ctgaagtcaa tgcagagcaa ttaaaagttt tagatgcaca
    11221 aaaaactcct ggtaaaatta ttacaaattc aaattgcaca atcatgggag ttacgattac
    11281 tttgaagcct ttaatggatg catatggaat cgattctgta tttttagttt ctatgcaggc
    11341 tgtatctgga gctggttatc caggagtccc ttcccttgat attttaggaa acgttgttcc
    11401 tcatattgga ggagaagaag aaaaagtaga aatggaacca caaaaatgtt tgggcaaagt
    11461 aagtggcgga aaaattgttc ctgctaattt taaaatcact gctcattgca atcgtgttcc
    11521 cgttgtagat ggtcatactg tttgtatctc agttaagcta aaaaagaaaa caactgcaga
    11581 aaaaattaaa aagctttggg ctgatttttc gggtgagcca caaaagttaa agctcccatc
    11641 agcgcctaag caggtgattg attatagaga agaaattgat agaccacaac caagacttga
    11701 tctaatgagt ggaaacggga tgacaactac tgttggtaga ttgagaccag atccaatttt
    11761 tgattttaaa tatgttgtgc taagtcataa tacaattcgt ggagcagccg gtgctgctat
    11821 tttaaatgcg gaattaattt ataaaaaagg ttacttgaga gtaaaataaa ttgtcagaac
    11881 aaaattcaca attctttaga gtagttgatt ttaatccagc tgcgcaaagc gtgcagggaa
    11941 tggaatctcg tggagatttt cagtataaag gcatctatcg tttttttacg accattaatg
    12001 aatggacgga aaaatttcta gcaaataaga ttttgccgaa tgcagaacaa ttaactcgag
    12061 atattagcat agaaaaagac aaagttgatt tttttataaa tgaactttgt tttaaagcaa
    12121 atcctcctat tattaaaaaa attacgactg tagaatttga attaagtgat attcgtaagt
    12181 cagaaaatct ttcaacagga ttgagaagga atacggtatt cgccagacct tcagcattag
    12241 acgcaggatc ctgccagaga tatatcaatg gttgtaatga aagaagtgtt gctgccatta
    12301 aacgggcaat tgcagaagta agagttcctt ggcatggaga caaatttaaa gattatattt
    12361 tcacaaggat caatgcaaat cgtttgagcg atacttatgc atcttatgat attggaaatt
    12421 tatttaattg tccttatgat tcaactaaac agttaaaaga aatgactgta aatatgcact
    12481 taaaaccagt gctcaaaaag ttagtagatg ataaagtcct tctgtttttt agaaatgaaa
    12541 aggctctcaa atcggcaaat aagtctgtat ttctctataa cagtaaagat gaaatcattg
    12601 agcgaatcga aatttatgtg aattatatga aaaagcatat cattccttct ttacaaaaaa
    12661 ttggagtgat agggggagtt tctgacgagg aatatgcaga ctttgcttct ctttccacaa
    12721 aaattttagc ctacatggac gattcttacg gggatcagaa atatatggtt gaagaactga
    12781 taattctagg tgctttctat gaaaactatc gtgaagaaat taaaaagaaa gagcaaaaag
    12841 ataaagtcat agaagttgtc aagttagttg aaaatgcagg taagctagtc gatctagcta
    12901 ccatacgtgt caatggggat ccaattccga aagagttgat tccgtctatc ctcgcaaatg
    12961 aaaatattct ttattgcgaa tatgatgatg gtagttctct ttttgaattt ctattacata
    13021 aatctgctat aaatctcgcg gctgacttgg ctcgtaagat tttccaatcc actggaaatg
    13081 atacagatat tcgaattctc tctcgtatga atatcaatac cgcactagat gaaaatatca
    13141 aaaaagattt ttattcagcg gaaatggaaa gtttgtttaa gtatttacca tttctcactc
    13201 gactttggcg tatgataatg ggtaatatct acgttacaaa agaagaagca gaacagattc
    13261 gccgccaaaa agaaatggag caaaagaaac gaatcactga atctaaatca aaacaaattg
    13321 caaaagaaaa gtctagactt gtagaagaaa gaatgaaaca gatagacgaa attgagcaag
    13381 cgggaacggg tgctgatgcg aaagaagagc cggataaaaa actaacgccc gaagaagaaa
    13441 aacaagtcaa agatgtttta caagctatca ttacagcact ggattctgct tgggatgcaa
    13501 aactttatcc tgacagagag tatctcatgc aaaacgttaa gactggcatg agtgaagacc
    13561 agtttgttat gtttatgaaa aagcattccc agaaggaagt tttatcattt caaataaaat
    13621 ccaaaagtaa taaatataaa tggcctatac ttgtatctag gcattattta aagcgacatg
    13681 gaagacgtat gctcgagatt gcaaagagag aatcggatac ggagagaaga tctccagctc
    13741 ccgatcagga taaatttgac ctattttctc atctggaaga atttctagaa agagtattac
    13801 cgagaatcta atgcacggtg tagagcgatt tagaaaaacc aaaattattt gcacgatcgg
    13861 accagcgaca tccgacatca atatgattcg caagctggct gaagcaggaa tgaatatcgc
    13921 acgacttaac atgtcgcatg gcgatcataa attccatggt gaaattattc agaagatcaa
    13981 aaaactgaat aaggatttaa aatatccaat tgctatttta attgatactc aaggacccga
    14041 gattagaacc ggggaagtgc ataccgaact tgatttaaaa gtgggtgaaa tttttacctt
    14101 tcatgtaatt ccaggtcaag agtcagaaga gaaatcagtt tttgtaaatt atactgatat
    14161 catcaatgat ttaaagatag gcgataaagt taccgttgac aatggattaa tcaaccttgt
    14221 tgttcttgag aaaaaagaga aagaacttgt ttgtagagta ttggatggag ggaagcttgg
    14281 aagccgtaaa catatcaatc ttccagggat taaagtaaat attccatcta tcacacaaaa
    14341 agatttaaaa gatatattat ttggtctaga acaagatatt gattttattg cactatcctt
    14401 tgttcgaagt caggaagata ttttacaact tcgaaaaatt atcgaagaaa acgaagcaca
    14461 tgcacaaatt atcgcaaaga tagaagatgc cgaagcagta aaaaattata gagaaattat
    14521 tgcgtcagcc gacggagtca tggttgctcg tggagattta ggagtagaag tagaactcga
    14581 agaacttcca atcatccaac gtcgtattat aaaagaatgt gcatttcaag ggaagagagt
    14641 aatagttgcg actcacttac tagaaagtat gattcacaac ccatttccta caagagctga
    14701 ggtaactgac gttgcaaacg ctgtgtttga ggaagtcgat gcaatcatgc tttccggtga
    14761 gactgcgtca ggtaaatttc cagttcgttg tgtggaaatg cttcataaaa ttgcaatgcg
    14821 tattgaatcg tcaggcggtg gggtaggtta cgttcacaat cgaagaccta gaaataagaa
    14881 agaagaattg gctagatctg cagctcttct tgctgactca ctcaaatgca atgcagtcat
    14941 tgtgattaca aggcgtggaa ctactgctaa taatatagca gcctaccatc ctgagcatcc
    15001 aattattcat gcatttacaa atatgacctc agttcgtcgt aagctttggc tcaatcgtgg
    15061 agtattacct tatcgtgtgg atttttctag tgatccagaa aagacaattg cgcttgcgat
    15121 tgaaacgctt aagaaaaaca acatgattgc gcgggggcaa caggttgtta tcctctccga
    15181 tatcattgct ggggaagatc gggtagagac gattcaagtt cgagacgtaa aataaccgcc
    15241 tgattttgtt tcgtttttaa agattcccac ggataaacac cgatgaaaaa tgatataaga
    15301 ctccgatttt ctaattttat ttgtgtttat attacgacat tcagggagag tccttttcta
    15361 aagcaaattc aatgagtttt gtgtgggaat taattgctta aatgaaaaaa aatgtatttc
    15421 aattctttct tgcgtttgca attactcttt ctcttgcatc tatcgttcaa gaagaattct
    15481 tagacaataa agaaatcttt cctcacttca aagaatatct cccagataca ttagaagtgt
    15541 tagaaaagga attcaaaaaa gaacctccaa aattagagca agtcaaaaca aatctatatg
    15601 caggaaaaga ttatgagtat ttaaattctt tttttgaaaa gctctcgagt cttgaaagaa
    15661 gcaaagatag agttatccga attctgcatt atggagactc gatgttatgg tcggatatga
    15721 ttacatctga gatccgacaa agatttcaga aagactttgg ggatggaggt agaggtttag
    15781 ttccaattac aaatcaactc tctcgaaaag tattgtcaca taaaaataaa acggatgaaa
    15841 ctcaatttac ttggaggaac ttagatcaca gacaatttgt aaatccacaa attggattct
    15901 tgggagaagc ggctattccg aattattcgt ttgtctcaat gcgacatgaa atagactcag
    15961 agattccatg gtcaaatgta aaattgtttt ttagaagtcc ccttccaaat tctcagttca
    16021 aatcaaaagt attatataaa gtaaaacaaa tttcaaatac ggttacactg gaagatattc
    16081 cagtgaaaga ttgggaaatt agtgattcca aggattgcaa agaaatggag tttgttttag
    16141 aagatggagt ctcctttcag ttagatatta cagggatgaa ttcaactccg atttttgatt
    16201 caattcaatt tgaaacaaaa gctgggattt cgtattcaac tgtttctagg caaggaatag
    16261 aaattagtga tttattgtta atagaagaaa aaagtttaac ttgtggattt acgcgatata
    16321 aaccagattt aattgttttt cagtttggtg taaatgaatc tgaaaattta gcgcgtaaag
    16381 cttttattac gccagaaaaa tatgagaaag atttagaaga cgttatccgg cgctttcaaa
    16441 aaattttacc aaacacaaat attctactta tttctccaat cgagagaata tcaaaaaatg
    16501 agaaaggcat ctttcaaacc atgcctgaaa ttttatttat cagagacaaa caaaaggaaa
    16561 tcgcagagaa aaacaaaata gctttctttg atagttttac tgctttaggc ggatctggtc
    16621 aaaatgatgt gctatatgaa aatggattta tgcagccaga taggactcat ttaactcgta
    16681 aaggagggga gttctttgca gaaattttct attctgattt ttttactcat tataagaaat
    16741 tcaacgggat tgtagaagaa accaaacaag cgagtctaga aaaattaaag aaagaaaaga
    16801 acaaagaagt aaattttgtc tccaaggctt atttttattt tttcctattt gtttggattg
    16861 tctcatccct tcttacaaga tatccggaat ataaaattgg atttctagcg attgtctctt
    16921 tttattttta ttctacatgg aatttctatg cagtctttat tattttactt tcaacggtta
    16981 tagattattt ttgcggtctg caaatatata aaaaaagaca aacgggaaag aatggtagcg
    17041 gctatttaat attatcctta ttgtctaatc tttctcttct tttctttttt aaatactatg
    17101 acttttttgc aggtctcttg aatggagttt tggtaaatgg aaatccaatt caaattcctc
    17161 ttttgcattt gattttgcct gttggaattt ctttttatac attccagact ctttcgtata
    17221 cgatagacat ttggcgagga gtattggttc cagagactcg aatgtttcga tttgcagagt
    17281 ttgttgcttt tttccctcag ttggttgcag gtcccatcgt gcgtgcaact gagttcttgc
    17341 ccggtcttaa aaatagggga gaacattttt atttaaactc aattcaattt tcaaacggtg
    17401 tatttcaaat attagtcgga gtcttaaaaa agattatagc agatcgctta gcagcaggaa
    17461 tagtagatgg agtttatgca aatcctgata tgtatacaag tcctgaaata ttagctgcga
    17521 tctatgccta tggggtgcag atctattttg atttttctgg ctattcagat atcgcaattg
    17581 gttctgctcg tattttaggt tataacctta ccttgaattt caatcgacct tatactgctt
    17641 actccatcaa tgatttttgg aggaaatggc acatatcgct tggaagctgg ttcagggatt
    17701 atctctatat accgcttggt ggaaataaat catttgtata tcgtaattta ctgattacat
    17761 ttatactctg tggaatttgg catggggctg gtataccttt tttaatttgg ggaatctatc
    17821 atggagtttt gctagttatc gagagagggc tcaaaattga ccggaaagag aattatttga
    17881 tgagactcat aactctacat ttggttttaa ttggctggat tgtgtttaga agcgatagtt
    17941 ggcttacctt tcagaaatta attcaaaatt tatttagctt tcatatgaat atacaaaatc
    18001 tagaactatg gattatttta ttcttgtttg cctattatat ttatgaatgg attgggttta
    18061 attttatcaa tcggattcaa tctttatggg agagttcatc ttttcttttt caaggtgtat
    18121 tggctgggat tttgatttta attgcttatt atttgcaccg agtggagata aagccattta
    18181 tctattttca gttttagtgt atttgagtaa aagtggctat tcaccgtctg ccttttgctt
    18241 gacagtaaat acaactggaa tgaaaaatta aatatgaagt atatttatta tatagccccg
    18301 atagcacttg tgttgtttat aggcttttct ttttttagtg attttacaaa acccaagaaa
    18361 aaaaatgcaa gcacagcact cgagagggca gaagaaatgt attcagaaag tgcaaatgca
    18421 aagagaaaaa aaagagcaag cggcaaatct ctgtttgatg ctgattctaa attcttagaa
    18481 tttccggaaa ttggaaatga agaaattgat ttaaatgctc ctctcacacc agaagagcga
    18541 gaaaagagaa gaaaatttat tatcgagaaa gctaaaaagc ttgctgatat gtttccaaat
    18601 aattctgtca ttccaaggca tttatcaccg gatgctttaa aaaagttaga agagacaaat
    18661 caacgaatgg catctataca aaaccaactc atacaaaatg aaaaagtttc taaagaagat
    18721 aagctttttt actataaaga aagacttaaa acatctaaag acagactcga agtatttagg
    18781 tatgctcttg gtctagaaaa taacggtgtt tttgatgaat caaagttaga aggattttta
    18841 aaagaacgtt atgatatatt tgttgagaat gaaaaagcct acgcagatga aatcataaaa
    18901 gtagaaaaag aataaaccga gaggactaaa atgaaaccag ctttattaat tacccaatgt
    18961 ttacaaaatg attttacaaa attaatcggc aaatttgatc cattcccaaa tttacttcat
    19021 ataggatatg atgaagctcg gaggatgctg agcgaaaaaa tagaagaggg acctgttaca
    19081 acggtaatgg attgggcata tcagactcct tctgaaaaac ttacaattat tcatattaga
    19141 gactggcata atccaaatga tcctctgcaa aaagatcatt tgcaacaatt tggtgcacat
    19201 tgtattcaaa acacagaagg agctgagttt gtatttgcaa agtcaatact accaaatcga
    19261 cctcatcata tagttaacgc atccggtcta aatgattttg tagatacaaa tctagaagaa
    19321 atactttctt cctataagga tgagccaatt cgtgtcggga tcatgggtgt ttggacggaa
    19381 gcgaaggtaa cctttcttgc ttatgattta aagactcgct atccgaattt tgaaattgtt
    19441 gtttgttctg ctctctgtgc aagttcctct agaaccatgc attttatttc gctagagcag
    19501 ttaacgtcaa ttcttggggt caaggtattt tcttctattg gagattttac aaattttctg
    19561 acaggaactc ttcctaagat agagcataag atccaagatt caaaaggaat acagattaaa
    19621 ttttcggatc caaattattc tgtgtcggat acagatatgc aaatactact ttatcttttt
    19681 agagatgcaa aagatgtaga gtttctttgt ctagatggag ggttctctgg aaatgttgtt
    19741 ttgaaggcaa aagcaattga taaatatggt catactcaag ttccaacagt tatcaagatt
    19801 gggaaaagag aattgattgg aaaagagaga acttcttttg aaagaattca agaagtatta
    19861 gggaataatg ctcctagtat tgtggattat gcagaattag aagacagagg agctataaag
    19921 tatcgttatg cggcaatgct tgataatgga gtgcttacat ttcaaaagta ttatgcaaag
    19981 atagacgata tggatcgtat taagcataag ctcgaaattg tattcaagag acaattaggt
    20041 agactttatg atgcttctac aaatgaaaaa ttaaacctac tctcctatta tgaatattca
    20101 aataaatatg cagcatcgat tcgaaataaa gtagagacta ttataggaac taaggcatct
    20161 ggcaatacac ttaatttaca tgggtatagt tttccgaatg tttgtaattt ttatgaaata
    20221 gatcttgcta ccttacaaga aaatataact ccgtatcatt ttgtttctta tctacacgga
    20281 gatttaaacg gggcaaatat tataatagat gcacaagaga atgtttggtt gatcgacttt
    20341 tttcatacac acaaaggtca tattctaaag gatttaatta aattagagaa tgatatactt
    20401 tatattttta tgaagattga ctctttggag gaattcaaag aggcagttca tcttattgat
    20461 attttgttaa gtgttccgga tttaggcgaa cctttaaatg aaagtatttc gaatcaattt
    20521 acatttcctc aaattataaa agcatctaga accatcgctt atctcagatc cttttatcca
    20581 gatttgatcc atacggatag ggatccttat caattatttg taggactcat gcgttattcg
    20641 atgcatacat tgtcttttga tgaatccaat gattggcaaa agaaattggc tctctatgca
    20701 ggttcaattt gttctgcaaa aattaaaaat actctgcaat ctaccaaaga tttacgcatt
    20761 gattttattg agaatccatc gaatcaaaat gaaggaaaga tgggtcttac aattttacct
    20821 ggcagaaatg atagagcaag aagtattgca gaagatttaa gagtcattgg tgaaaataat
    20881 attacagcag ttatttctct gattacggaa ccggagtatg aagagtatgg tgtagcttct
    20941 ttaaaagagg catataaaaa cgcggggcta acaacgtgtt atttcccaat catagatcaa
    21001 ggagttcctg attttatcac tctagataaa aatttaaaat ggatgcatga aattctgtct
    21061 aaaaaacaaa acgtattaat acactgtgtg ggaggtcttg gtcgatctgg aacagttgcc
    21121 gcttgctact taatccgtta ttacggactc agtgcaaagg atgctatagc ccatgtgcgg
    21181 aagtatcgct ctgagcgagc aattgagtca atagagcaag aaaaattcat agaggaattt
    21241 gcaaataagt agaattatga catcagcaca gaaaaaaaag gtaggatcaa acgaaggaat
    21301 ggaagaatta gttaaggttg aagaaattca aaaagcaaaa gaaaaaatca atggactact
    21361 agaagaacta aagaaagaaa ttacagggca aacagcagtt attcgcaata taacaacttg
    21421 tctagttgca cagggacatg ttttgctaga aggaatgccc ggacttgcaa aaacacttct
    21481 cgcaaaatcg attgcgcaga gtatcgattt atcttttaaa cgaattcaat ttactcctga
    21541 tttacttcct gccgatttaa taggaactgt catctttaat ccaaaaacgg caaattttga
    21601 aacaagaaaa ggtccaattt ttactggaat tcttctcgcg gatgaaatca atcgtgctcc
    21661 tgcaaaggtt cagtctgcat tgttacaatg tatggaagag agaatggtta ctatcgggga
    21721 aacaacgttt cagttagaga ctccttttat ggttctagca acggaaaatc ctatcgacca
    21781 agaaggaact tatcaattgc cggaagcaca aatggaccga ttcttaatga aggtgaacgt
    21841 tggttatccg gaagagtcag aagaaattgc aattctagga cagcatggaa gtgtttccgc
    21901 gaagagtaaa gaaattaaaa aatgtctcac tcgaaaggaa gtgcaagaaa tttcctcttt
    21961 atcggataaa atctacatcg atgagaaatt aaaaaattac attgtgaaat tagttcgaaa
    22021 cacaagaccg caaacttcta agttagacga aataaagccc tatgttaagc atggagtttc
    22081 tcctagagcc tccttggcac tccttcgctg tgcaagagca aatgctcttt tagaaggtag
    22141 aaattttgta attccagaag atattcgatt ttctatttat gaagttatgc gacatcgtat
    22201 gattttaaca tttgaagctg tttcggaaga tgtaacagtt gactcattaa taaaaacggt
    22261 tttagaagta actgagcttc cttgacttgt ataagtaggt aaagtctttt gatactcaaa
    22321 gagttagaaa agttaattca ggaaattgaa ttaaaataca aaaagaagat tcgtagtaac
    22381 cgtgcgggat caattcttgt gaatcgatca ggtagaggaa tggactttaa agagtctagg
    22441 gtctatatgt atggagacga tattcgcttt gtagattgga atgttagttc tagaatgggt
    22501 gagctatata ttaagctctt tcatgaggaa aatgatcgaa cgattaatat atttttagat
    22561 atatctaaat ctatgtcctt tagcggagcg aattcttatt ccaaattttt tattgggttt
    22621 cagtttttag catttatttc tctgctttct gtcatgactg gagatcgtgt aaatatagtt
    22681 ctctactcag acaagattga gtatgttgcg cctggaataa aatctagagt agatgcctat
    22741 cgtattctcc gtcgtattaa cgaaacaact ccaaaaggca agactgatca tttactccca
    22801 ctccaatatc taaaaaataa aattccgaga aattcgattt cttatattat cagtgacttt
    22861 tctggtataa acgatttgag tccgtatcga acactattgg aaattcatga actatacgga
    22921 attcgaatta gtgatccaat tgaaaatatg aatcatcctg ctctgaaaag tttttttgtg
    22981 caaaatattg aaactggaaa tggaggagat tattcctcta cttataattc agattcagat
    23041 atactaagcg agttttatcg atctaattta ctgaatctta gaacggattc agatttagga
    23101 aaatcaatag tgcggttttt aaataaatga aactttttat tctattattt ttatttccta
    23161 tactattatt cgcagaggtg atagaaagtt attctaaaaa atctgtccag gtaggggagg
    23221 aatccatact agagataaaa ttccaaacag gagatgtggc agaatggagt ttgcctcaaa
    23281 aaggtttttt attctctgaa tctgatgcag agactccaat cggagaatta aaagatatta
    23341 ctcaaacacc aacagaatta aagtttacat atgtatattt tgaagccgga aaatttagtg
    23401 caaatgtcag ttggaaaact tcttcaggtg aaacattcaa aagtaaagag gaattagaag
    23461 tcaaatcagt gttatctggg gagaaagaac caatcgatat tgcggaacct tttgtttttt
    23521 ctggatctta tattttgcgg ttaatcggta ttattttatt tggaatagga ataatttctg
    23581 ccttcggata tgcacttttc ttttttaata ataaaaagaa agttcaggca aaggatgcta
    23641 tatttgaaaa attagaaacc gaacaagcag ctgattacta taaaaagcaa ttacagcaat
    23701 tgctattaaa tacagaaatt tcacataaag agtttatatt tcatttgtca ggttatatta
    23761 aagagagaat cggtgcaaaa ttagatactt ctgtctttca tttaacgcaa acagaaattc
    23821 accaaatatt acaggataaa tatttcgtcg ctaatattga acttcttaca attgataatt
    23881 attttaattc tgtaaaatat atgccgaacg aagaaatgat tactcgtgag aatgcattgt
    23941 cactcattaa atactgggaa aagattttag caaaatgaat tattttgaaa gacctgaact
    24001 attgtatcta ataccattcg tgattttata cgctttttta ttttatagat ttaagtttca
    24061 tgaaaataaa atgaaattag atttaggaaa tcaatcaaat gttcctaaat ttccaaccct
    24121 agaaactata aaaatttatt ttccattctt acgatttatt attctcattt tattaatttt
    24181 atctttggca ggtcccggat ctcaatctag tcttctgcca gatgaaaaaa atggagtaga
    24241 tatgatgatt gcgattgatg tatctggctc tatggttaga agcaatgatt tcctgcccaa
    24301 aaatcgccta gaagtttcga aagatctaat taaaaatttc ataaagaaaa gagtgaatga
    24361 tcgaatggga cttgtcgtat tcggtggagc ggcatatcta cagtctcctc ttacaaatga
    24421 tattgagtcg ttagtagaaa ttgttagcga agtgaatcaa aatacagtgg atgaacaggg
    24481 aactgcaatt ggagatgcaa ttatactcgc tacatacaga ttaaaaacat ctaaagctcg
    24541 ttctagaata ttgatcgtga ttacagatgg ggtttccaat ataggaaaga ttgatccaat
    24601 tactgcagca gatactgcgg acaaatttga tataaaaatc tatacgattg gaattggtaa
    24661 agatttacaa ggtgtgttcg aaacagattt tgatagttta agacagatat cagagaaaac
    24721 agatggaatg ttttttcgtg caaccgattc aaatgaattt acagaagttt tgaattctat
    24781 tgatacactt gagaaagaca acttaaatgt gaagcctaaa ttatttgtgg aaccttatta
    24841 tatgtatttt ttattaccgg caattctact ttttattttt gatattatga ttagaagttt
    24901 ttatcttagg tattatttat gaaaaatttg gatcctagct atttcatact aatcgttttt
    24961 ttattagctt ccttttgtgt ttatctattt tataaattga agcaaagaat tgaaatcgaa
    25021 aattttctga aagtgtatac aggtctatca tctaaggtta ctctgccacc aagatggata
    25081 cttatacttc gatattcaat ctattcaatc attgtaattt tgctttgtat ttctattttg
    25141 aatccttctt tctctgaaga atcaggtgcg gacgaaaaaa ttatctctgg cgttgatata
    25201 gtatttttgg tagatgtaag tctttcaatg aatgcgactg acgttttacc aaatcgactt
    25261 tctcaattta aggaaactgt tttgagacta ttgccttctc taaatggtaa tcgtcttggt
    25321 atcatagcat ttgccggtgt cccatttctt tactgtccaa tgacgaatga tatagcagcc
    25381 ttctcagatt atattagagg tcttgacgtt gatatgattc caaatacagg aaccaattta
    25441 aaaaaggcat ttgcaaaggc ggaggaaata tttaaatctg gaaaggttct cagaaataag
    25501 gtcttgattt tggttacaga tggtgaagac ataaaaggta gcactcctgc aagattaaat
    25561 gctgatttgg taatctgggg agttggaaca gaagagggcg gtggtatttt ctataaagat
    25621 gaacaaactg gaatcgccgg atacgttact cgctctggaa acttaatagc tgataaatcg
    25681 aatacagatt taattcgcac taaattagat gaagaatatt tgctagagct tgcatctatt
    25741 cagaaagcag actatagtaa tatttctgct caaccacaga gtgcagataa attaatttca
    25801 aaaatccaat ctatgtctaa aaatacttct tcaaaattga gcaatatgtt taaaaaagat
    25861 gggtatcaat actttctatt tccagctttg atactttttc ttttagatat tagtttatta
    25921 gaatatttat ttcgtaaata tgcaaacatg cattcaaata actaggttga aacaaactat
    25981 tttaaaatgt tgcgcgcttc ggcagaagca taaaagccct ctagtcttgc tgtgattctt
    26041 ttttgtatat gagattgcac ttctggaaga gataaatact tcgttaaaat ttttattaga
    26101 ttatcagtga tatctttttt ttctacttta tagagtgata agatttcttt tattttctta
    26161 tctttgtatt gagtttgtat aacatcaata tgaccctcta aaatttgaat taacaaggga
    26221 gatgctttga taaattctaa ttctgatttt gcgatagata agattttttt tatatcattt
    26281 ttatttgcgt aatgttgtaa gtccgcaact tccttttttg aaataaaatt ccatagctca
    26341 tctgttaatt ctttcgatgt ctttgcatct aattcttggt tcagtaattt ctcgctttgt
    26401 tttaaagagt tttgtaaatt ggcttgaata aacgcagtaa cattcttttc tatgtttgct
    26461 tgcattccgg gtatgtttcc tagaaaatct tgtcctaatt taaaaagaga ggaggcgacc
    26521 ggaacatttt tagcaattga attttctccg ccgataaatt ctgcaattgc attgtataaa
    26581 acactcgaga tcatctttgt gtaaacaggc gagttgatga tattgtgcac aacgtctttt
    26641 cttagctcgg tcatgctaag acccatattc atgctttctt caaaaatact tttagtgaga
    26701 agggaagtta agggctcttt gctattttga atggtttggt aagtggattc cgatagattc
    26761 tgtgctaaat tttctagttc tttttgaatt tccctagatt gtagataatt tttggcggaa
    26821 tcaagaatgt aatttttgtc aattagatca ttaaaattta tttcgactat cgtatcgtat
    26881 aaactttcta cttcaatctg taaattgctt tttaattttt cgcctttcca tgctgaaact
    26941 tcatactcta tatgcgctgc caataatttt tctatttgtg acatttttcc ttacctattt
    27001 ttagtttgta gttagtcgaa agtgatttat aggttaatca gaataaaata tttagcaagt
    27061 ctttttattc tgattcatct aaagtaaaat ggatcgggat tcgatgtgtc atttttactg
    27121 gttttccttt tgcatagcca ggtaaaaatt ttgctttgtg aatgatttta atagctgctt
    27181 cttcaaaacc atatcctatt ttagaagaaa ctatcttggc gctttttaat gttcctgttt
    27241 catcaatttg aacagaaaga attactgtct tatttgtaat attcgctgtc atagcggctg
    27301 tcgggaaaaa ttgtttcaaa tcaaaatcga taataggagt cggtgctctt tgtgcatttt
    27361 gtgggatttg atacccatct ttatcaattc catttccaga caatgcgttt aaatcaatat
    27421 tttcttctat tgaatcctct ttaattcctt tctctgtttc tatccaatct tttactttca
    27481 ctggggtggg tttagatgca ttgttgttta aagtggaagg aatatcttcg aaatttaaat
    27541 caatgtcttc atgaatattg atagaatcta aatctgtatt tgtcgcaata tataacgcgg
    27601 catatgcact aacgtgtata aagctagaaa aaaataacat gagcccaaat aagttttcct
    27661 taagcaaagc ttttagcctt tgttagataa attattcatc taacgtaaaa ttgataggaa
    27721 cccgatggga catttttact ggcttgcctt ttacataacc tggagagaat cgagccatgt
    27781 tgatgatttt aattgcggct tcatcaaaac cataccccgc tttacccgaa gcgatttttg
    27841 cacttttaag agctcccgtc tcatcaattt gcacaagcac aacgaccgtc ttgtctgtaa
    27901 tatttgcggc tttagcagca gctgggaaaa attgctttaa atcaaaatca ataataggag
    27961 tggggacttt atctccgttg aatgaaaata aatatccatc tttatccgtt ccatttccag
    28021 aaacggcatt tacactaatg tcttcatcaa ttggatcgtc tgcattttta ttgcttcctt
    28081 ctacccattc acttttctca acaggtgcag ggcttgacgt tccacctatt aattcagggg
    28141 gaatctcttc gaagtttaca tccatatcct gcttaatatt atcagaatct acttcttcga
    28201 tattgataat agtagaaata taataagcag cgtaaacact gaaatgaatg aagatagaga
    28261 gaaatagcat tgtgccaaat aaattgtttt ttataaatcg caaaaatttc tctttcatag
    28321 gtacctactt tttaacagaa agcgcaattc tagtcacgcc tgctttacga ataagcccca
    28381 tgagtttggt gattgttcca taggctaaat gttcgtcagc agaaagcgtt atacgcatat
    28441 tcggtctaag tttagaatct ctttctagag ttctaaataa tttttcttca tcagtcgatt
    28501 gtccttctag aagaatttta ccatcccgag taatagcaac ctgaaccgat tgagcaacgt
    28561 ttggatcggc tgcttgaact tttggtaaat tgatattaat agattctttt tttaagaaat
    28621 tggctgtcac catgaatata actaataata ccaagatcac atcgaccatt ggagttatgt
    28681 ttatatttcc aatttcatca tcatcgcctg attgttgtgc cattgccata attgtattct
    28741 ccttaaaaaa acaaaaatta ctatttaaaa tataggctca aatctatggg ttcgaaaatt
    28801 cccatttatc tgcgtccttt cgtgtttaaa ctcaatgttg ctttgcggcg caacataata
    28861 tggttaaaca tattttcttc attttctact gtaacttgca ataaattcct ttgatagaat
    28921 ttctaaattt tgagtaatta cttttagttt tttggaaaag taattatttg ccatgaccac
    28981 aggaattgcc acaaaaagtc cagcggcagt cgcgagaagg gctgtagaaa tacttttcat
    29041 aactacttct gctccggaat ttccaagagt tccaagacca ttaaatgcct tgattacacc
    29101 aagcacagtt ccaagtaatc caagaaaagg agcgttattt cccaatgtag ataagattgg
    29161 aagtcttttc tccagcatga ttctttcaga aattactttg ccatgcatca tttcacccaa
    29221 agcttcttgt ccatcattgt aatgtgttac ggaaaattct gcaaatcgag agtaaacgtt
    29281 agatgattga gtagaaagat ctgctactcc tttgatatct ttctttctaa gaagatcgat
    29341 aattcctctc attgataaat ctcccttttt tgtaaacttt gaaaatacaa cagatctttc
    29401 aaagccaacc gcaagagcca aaatacttgc aagtcccatt aatataaata ctagttgttc
    29461 acctaaatca acaagttgtt ccatatattt ttccttttat attcgttcta aaataatttc
    29521 taagtttttc tactttcatt tactataatt tttcaaattc tttttttcac attctgtttt
    29581 tctattaagg agcattgttt gtaacagaga tacaatttct tttacaagtg ctcgttccgc
    29641 tgtctgttcc aatacccgct gtcattgctg caatcgttcc agtgcagatg tttaaatttt
    29701 ttctagtttg ccagtagctt ctatcacacc taaaatagca ttctcggagt gaatcgctgt
    29761 tattcgtgac gaaatttgca ggcagtggat acatatatag attatttgca cacaagtcac
    29821 tataagttgt aaatcttccg ctgctgattt gaaaagaaaa gttggtctca tttacgagcc
    29881 cacctgaatc tgatgcagtc acaatgcaag cttcctcggc acgcattgca tcaatacaag
    29941 cttgctgagg atttgctctc ggtgtggtgg cagttactag taaaatgcga agtgcgtaaa
    30001 ggtctttata atctttctca tttgatttgg agcaaaaaga aaaagaaaga attataaata
    30061 cggttaaaaa tattctactc attaaaatct tgcctccata ccaacattga taaatggaat
    30121 taacatccca cttggtagct gtagtgtaaa aaaatcattt cgtggaccag gatttctttt
    30181 ggagtaggga gcagtagagt caaaatcttc tccgttcaca tttttacgca tgtagatgtt
    30241 aataagttcg aggtaaacat tgatatatcc ccattcataa gctagaaatt tatcaatacg
    30301 aatatcaaaa cgatgatatg gtatttgacg tctagaattt gaataatcat tggagtaagg
    30361 attgtttgaa tatctaggat tccaatagat ttgactatta tatggatttc tgaattgccc
    30421 accgtcatct ccgatgactg gagtataagg aattgatgtt ctataaaacc atcttcctcc
    30481 tatttggtat tcttccgtta tacgccatcc ataaacaaca cttaataaat gagttatatc
    30541 ataatcaaaa ataagctcgc gagaatttgg gacaagagat cttaatctct gttgttctgc
    30601 acttaatgta tagtttctat tcggatcgta atcagcatta taaatgttat tgtttcggaa
    30661 tgattgagac caagtataag aaatccaacc aaaccaatct tttgaatttg gtcgatttga
    30721 ttttttaata tagatttcat atccatgcgc ccaacctgtt ccactattag aataattgag
    30781 cgctcgattg gtgactatcg gtctttgcaa tctttgatat ttatcaggat ttaaaccata
    30841 agcttctgta atataaggat catttacaat taagtttgta aattcttgtt taaataattc
    30901 tcctttgacc gaccattctt ctgttaattg ttggtctata ccaccaccat actttctggc
    30961 tttttcaaat tgaatatttg gattaccgga ttcacgtgaa atagcttgat caaagtaagg
    31021 ataacggttg taatccccag cacctccaaa tacagttgtg ccttttaaga ttccttcaaa
    31081 tttataagat gcaacaaatc gtggaccact tgctccttgt tttgaatagt ctacataatc
    31141 atgtcgaatt ccaggttcaa atttaaaatt tccaaatgta aagtgaaagg tcatgtatgc
    31201 attgaaatat ttagtctgtg ttttttgcgt aatgtctgct ttttcaaaat ctggatttgt
    31261 taaattgtaa ggatttggag caggattatt aggatctctt tggcgaatac tataaccagt
    31321 tatattatag ttcatgatcc ttccttctgt tccaaaatct atttttagat aactagttgg
    31381 attccaaact agatcttgtc tgattccaga ataaggagca cttgctctaa aatccgcttc
    31441 aatggatccg aatttaacag tggtattatt gaaaggttca tagttaatca atgtaatacg
    31501 attactgaat tttgtgctcg gtgtccaaat gtatcgcagc cccattgttc taaatccctg
    31561 tcctgcggca acacttgcac ctgctacagc ggataacggt tgctttgtag gatcatttgc
    31621 cccataaccc ccaggagcat ttaatacaaa tccatctaat gcagttagag atgtaaaaga
    31681 aatttgatga tttgcattga aattataaat aaatttcaca ttggaactgt tgtattgggg
    31741 gagtctgatc ccatcaggaa tgagtccggt ggatcccaga gttttatcta aatatccaat
    31801 ttttccacct gctgctaaat atcctttacc gtttaagatt ggagtttgat acatggcttg
    31861 tcctaaaaga aaagacatat tgaatgaacc agttgctttt tttgctgcct catttgattc
    31921 aatttctaca acgcccccag tcgcattatt aaaattggct ggatacgctc ctgtataaat
    31981 atctattgtt ttaattaaat cattatggat aacagagttg atagtatcaa aatggtaagc
    32041 gtaataaatg ggtaaatcat catacaggta tgtattcatt ctacaatctg ctcccctgat
    32101 cactatccca ccaaaacaac ctccgttccc acctccaatc gccggaggag cgaatactcc
    32161 gggtagtgtt tgaatagcgt ttaacgcttc tccaagggtc cctggcatac gttttatttc
    32221 atcgtatctt actttgtaac gagataaaat cgttttgtct ttctgtccct ctacatcaat
    32281 tccgcctttt ggttttggcg ctttatctgt ataaagagtg attacttccc catcggctgt
    32341 aactgctttt tctaattctt gtatgcctgt tggtctgaga actctaaatg tataggttcc
    32401 aagggcaggg acttctacgt caaagtaacc ttccgcatct gtctgggctg gtatttttgc
    32461 attcggcgtg actactacca cagtcaatcc cgcttctccc tgatttttag cgcgactaaa
    32521 aagtcttcca cgaaatgcga cagccgatac ttctgagctg agaatagaaa gtaaacctac
    32581 aaagagtaga aatttatatt tattgtaaaa ttttgaattc atagtattac tttatctgcc
    32641 tctttgctaa atcattattg aagttaagat accaaggtat atcacctaat ttttctgttg
    32701 tataaatccc aagacagatg ataggatatc caagaaaagg acaactagtt cgggtaattg
    32761 caatactgca tagatttaga ttcctactgt cttggtcagt gaaaaccaga aggggaggtt
    32821 gtggaggtcc tgcttgttta cattcacgag ctttgaaatc agccgccgca ttgatttgag
    32881 attgagcatc ccatgctgga attggtttct cgttgcaatg aaagaaaaaa aataaaatcg
    32941 ttagaatgat actagaatgt aagagtctat ctttcattat tttgatttcc tattctgcgg
    33001 tttcttgttg ttagacggtt cttcgaatag aggagcttga gtatctgttt ttagcgatac
    33061 cacatctccg ctcactttgg ttacagatac tgttccgagt ggatagaaat aatgtgtctc
    33121 atgccaaaag gtcatattaa ccatagagtc tccgccttct actttttgaa ctgcttgact
    33181 cattgcataa tccatgttat gcggattggt caatgggatg agtccaaata aaaagaaagt
    33241 tgaagtttct cctgtttctc tttgaagaat tttatatcct tctgaacgta ttaaagttgc
    33301 tgatggtgta atatgttgat tatccgctct acgattgaga tttgttggat atgatacaac
    33361 cataatacaa ttgagtagca gtgataatat aaatatgtta acgagttttt ttagcatttg
    33421 gttctgtttg aggttcatct gtaaatttaa ctgcttctgc attgattcca agcctattcc
    33481 aagttaaaaa tagaaaaatg cttctatctt gccaataacg tatattgatg agagcatcag
    33541 cttctttaga attaatcatt tcttgaacta gctcattgat tggaggagtt gaaaaagaaa
    33601 ctgctagaat tgctatatct atagtgaccc atgactttct tccctctaca ggactgatta
    33661 ctttgtattt tttatttact attggaatgt tactagttga aatgcctgca cttgatgatg
    33721 cacagtttaa aagaaatgca attaatatta aatatatgtg tttactcatt gttagccctt
    33781 attcatttat agttttactt gctctgtttc cgtccagcct gtgctgaaga gtttaaaata
    33841 cttttccact tttctaagag ttcttgagac acctctttat ctaaattgat ccaatgcaaa
    33901 agaccgatta acttctctgt agaactccct ttttttttta aattttcaat tgcttccgct
    33961 actacttctt ttaggtagtc atagctgttt aatttctttt gaatagataa ttctgctgct
    34021 ctatcagaaa ttcggaaata ggatttcctt tcccctgaaa tacgaacttc ttcgatacct
    34081 gaattatgta taagcatttt caaattagtt gaaatactgc ttcgactaac tccgagaatg
    34141 cgctgaatct cttcttgtgt aattcctgaa tcggttataa gcattagacc tacaatttgt
    34201 ccacctattc tagaaattcc aaaactttca taatatttgc ccattttatt tacaaatgac
    34261 atcatttgtg ggtcaaaaga aagtttatca ttcttcttag aaattttttt ctttgttttc
    34321 ataatcctct gacgaacata tcacaaattt ctgtaatttc agtcaagact gaaattacag
    34381 aaaatactaa aaatctactc ttttcctttt tgatttttta tgtattgatt agacggagga
    34441 attaagtttt gaaataaaaa gatcttgttt tttcaatcga aaatagcctc ctatctattt
    34501 atcttttaga attaattcga ttgattcaga attgtgaaat cccaaaaaaa agaacttaag
    34561 ggatttggag ctttctaatt tagcaatcta gaaattcaaa aatgaagtgc tgttatctca
    34621 ataaataggt aaaaatttga ttagtcgatt ctaatctaag agagtgaatt cataaaaaag
    34681 tttaagtttc agatctacct atttattcta gaattatgct attgagatga aatactcgaa
    34741 tcgattcgta aaatatcttt gaaatccaaa catcttttta gatagtaaat cgaattccat
    34801 tcttgttatg atttagaact aagcgagaac agaattgatt tctttgaaac aggattagca
    34861 ttgagtctat ccagagctcc aatcagattg tcttggtaga attggtttta ttctttcctg
    34921 tttagaaaga gggtgtaatt taagaatcca tagtttggaa gccgatgatt aaatagaaat
    34981 atttcttttc tgaaaaaaat actcaaatca ggaaaaactg taggaatcca atattatgaa
    35041 atacttacta attttacttt tctcactctc tctcgctgcc caatctgcga aaccagttac
    35101 agtaaagaca tttgatacgg ttgttgactc aaaaacagaa accaaaaaag aaaaagagaa
    35161 accgaaagag aaagagattg aaccaaagga aagtagttca ctagatgatg atgaattaga
    35221 taagttattt tatacctatt actatttaaa tcttccgaag catttgcaaa aagaagacaa
    35281 agataaaaag aaactccaca caaatctaat aggtgttaca aaaaaggaaa ttgcgaagag
    35341 agatcgatct gctcctaaag aagacatcga taatatttct ccttccacag ttaagtttaa
    35401 aagagtgaca gaagaaagca agtggatgaa aaaaataaaa gctgaaatga aatacataaa
    35461 tttcactgag tatatgtata tcgttcgata taagcactat ttagttttga ttatttttca
    35521 tttagatcct gaactccatt tacaaagtcc agcacaagtt gagtttttaa atgttcaaaa
    35581 aacagagcaa gcgcaagatc tagattagtc ttcttggcta actcattttt cttttaaata
    35641 ataggaaaaa tgatcctgta acatttataa aaatggaaaa accaattcca aaaataccaa
    35701 ttctctttaa aaaattataa tttggtagaa tttgcttatt tccatctatt ctagataaga
    35761 ttttttcctt tccggcgagt ttaaattttt tttggcgggt aatgactgta ttcccaactc
    35821 taagtcgcaa atgagtttta tcatctacat attctgtagt ctcatgtagc ttgccaaatt
    35881 catctgttac catataagta taaatatggc gtttttgatt tccaattatg actgtatgat
    35941 atcctgttaa agtggaaaag gatttttgtc cctctcgctc taaaactttg taggcttcaa
    36001 tcagttcctt ttctctatct tggacgagaa atataaaacc tgtgaaaaca aggagagtaa
    36061 aaattccaac tagaaaaaaa atcttgtata atggtgtata caagctgtta gtaatttttt
    36121 tctctcctgt ttcttctacc aagttgacct taaactttta ctcgtttctc caagatgtct
    36181 agaatcttgg cagccgcatc tggaatcaca gtccctggtc caaagatcgc agcaacccca
    36241 tgctcataca agaaatcata atcttgcgga ggaattacac caccagcaac gatcaatata
    36301 tcgcctgcat tttccttttt aagtatctct ataagctctg gaattaacgt tttatgtccg
    36361 gcagcttgtg aagaaacgcc tataatatga acatcattct ctatcgattg ctttgctact
    36421 tcatttggag tttggaagag gggagccaca tcaatatcaa atcccatatc agcaaaagaa
    36481 gttgaaatta cttttgcacc tctatcgtgt ccatcttgac ccatcttggc aactagcatt
    36541 ctaggtcttc taccttcttt cttcgcaaat tcttccactc tcttgatgat cgcttggaat
    36601 ttttcgttgt cctgatttaa gctagaatac actccgctga ttgttctgat aacagcttga
    36661 tgtcttccga aaactttttc cattgcaaaa gaaatttctc caagagttgc tctctttctt
    36721 gctgcatcta ctgccaaatc aagtaagttt ccttttcctg attgacttgc ttccgtgata
    36781 gcgtttaacg ctgcatttac agcagactca tctctctttg ctttgatctc tttgattctt
    36841 gcaatttgtt tttcccgaac agcagtattg tcaatgtcta gaatttcgat tttgtcttct
    36901 gactttaatt taaatttatt cactccaaca atcacatcga ttccttggtc aatgagagct
    36961 tgtctcttgg ctgcagattc ttcgattttc atttttggta gacctgcttc gaccgcattt
    37021 gccattccac catgagcctc tacttcttcg aaaagtttct tagcttcttt tataatggaa
    37081 tgtgtgagag actctatata gtaagagcca ccaagtggat ctacaactct tggaatgtga
    37141 gtttcatttt gaataataag ttgcgtattt ctagcaattc ttgcagaaaa ttcagttgga
    37201 agtgccactg cttcatctaa agcatttgta tgtaagcttt gagttccacc taatacagct
    37261 gccattgctt caatggtagt tcggataacg ttattataag gatcttgctc ggttagactc
    37321 caaccagatg tttggcagtg agttctaagt gctgcactca ttggtttttt tggattgtat
    37381 tgtttaatta aatccgccca gaaaaaacga gctgctctaa gttttgcaat ttccataaag
    37441 aaattcattc caattccaaa aaagaaagaa agacgtggag cgaaatcgtc aatcgcaaga
    37501 cctcttgcta atgcagttct tacatactcc attccatccg ctaaagtata agccaattct
    37561 tgcactgcat ttccgccagc ttcttggata tgatatccac tgatggaaat ggaattgaat
    37621 cttggcatat ttttagaagt gtattcaata atatctccaa tgattctcat tgaaggcttt
    37681 ggtggataaa tataagtatt acgcaccatg aattctttta aaatatcatt ttgaatggtt
    37741 ccggaaagct cagaaatttt agctccctgt tcttctcctg ctacaataaa cattgcaaga
    37801 attggaatta cagctccatt catagtcatt gatacagaca tttcttttaa agggatctga
    37861 tcgaagagaa ttttcatatc ctctactgaa tcaatggcta cacctgcttt accaacatca
    37921 ccttttactc tttcatggtc tgagtcatat cctctgtgtg tagctagatc aaatgcaacg
    37981 gataatcctt tttgaccagc tgctaaattc tttctgtaaa aagcgttaga ctcttcagct
    38041 gttgaaaaac ctgcatattg acgaactgtc caaggctgat gagaatacat tgttgcacga
    38101 ggtccacgta taaatggaaa tataccgggg agtgtgtttg tatttcctaa attttctaaa
    38161 tcttcatatg tatagagagg cttgattaat attccttctg gagaaaccca gtttaaggtt
    38221 gatacatctt tgcctttaag atctgcttct tttgcgacct tttcccaatc acctaagttt
    38281 gctttttcca tgatttactc cttaactaat tcaatttctt ccatatgtcc agggtagtca
    38341 ccaacacctc ttgcaaaggt aaataaagca ggagctatca ctaaacgttt tcctgaattt
    38401 gtaactgttt cccaagaacc agtcgaaatt cgattcaaaa ccccaaatgg aagagtaaca
    38461 ataaataatg acgcgcctgt ggctaatccg actaagccga gaggccttac taaaatttct
    38521 gcaatcatct ctccactgct tggaacttcc tttgcagaga ttgatagatt tagaaataga
    38581 ctaattgaga ttactaatgc tagttttttt ctcatttcgc aactcctgat tttttatttt
    38641 taagatattc tggtagggaa actagaatag aacccacaag taaagagacg ataaccgcaa
    38701 gtgaaacttg tatactaatg tgaatctttt ctacatgcat gatctcgcca taaagaggta
    38761 gtagcatttt gaaacccaca aatgctaaaa taatggcaac tccatgtttt aggtaaacga
    38821 ataaatccat aatcccacta atcataaaaa ataaggatcg tagacctagt attgcaaata
    38881 tattagatgt ataaacgata aaggcatctt gtgtaaccga aaagacggct ggaatagaat
    38941 ctagtgcaaa cataacgtca cttgtctcaa tcattactaa taccacaaaa agaggcgttg
    39001 caagctttct tccattttct aaaataaaga attttccttc ttcagaacga gtggagaggg
    39061 gaacgatttt tttgactaat cgaaagacta gatgctcttc tggaatatag gtttcatttt
    39121 catcttcttt gtgcacaaac atcttgattg cagtgtaaac taaaaagaat ccaaagaaat
    39181 ataaaatcca agaaaactta gcaatgagtg tcgcaccggc taatatcatg attgcacgta
    39241 agactactgc tccaataata ccccatttta gaatatcagg ttgttctttt ggaccaactt
    39301 taaacttttg gaagatcatg ataaaaacaa acaagttatc aatggaaaga gaatattcta
    39361 gtaagtatcc cgctatatat tccattgttt tggttttact tctttcagga tcagagggat
    39421 ctgtatcata gaaataaatg aaagctgcaa atagaaagga aattgtgacc caaatcagag
    39481 tccaaactgt tgctcgtttg aatgacattg tatgattaga acttttattt aataagccaa
    39541 gatcgattgc taacattaag actatgataa tgttaaagat aataaataga atgagttcat
    39601 gattaatatt ctggagatac atacttcctc ttagtttcca aattaaaact tagtaaaagt
    39661 ttttacaata agaaattttt aaacatcagt ctttcggttt aagtgagtgt aaaaagacgg
    39721 taagtttttg taatttttct tgcgacggaa tttcatttgt gtctatctta ttaattgtct
    39781 tgttatagat ttcggataga tgctttttta aagtgtttaa agaaatatcc aattctttag
    39841 cgatgaatgt tcgtgatttc ccatcaacaa gagcacagca aatttgggat tgctgataag
    39901 ttaagccata ttctacttgc aggcgtgtaa ataatggtct acgaacagtt tctttgggat
    39961 ttgtatctgg ctttttttct gactctgttt cattttgaat ttctgtctgg gaaggaatgc
    40021 ttactggttc ttgggagttt gctttcatct tatctgctaa tgctaaagaa agtagaatta
    40081 cttcgatcgc agacccaatt tgtccgaagt gttcagctgt gatattaaaa gctcctaaat
    40141 ttgccatgat ttgaataaaa atagtaacta ataaaaagga ccatccaaaa agaaagaaac
    40201 gagctggacg atagccgata cgatatactt gaatggctgt agaaatgatt gaaataatct
    40261 tggctaaacc aagcaaatta gaaactttca tcaagatcat ttcatgacct agaattagga
    40321 taataatcag cagtagggtg gagattccag ttagtagtag taaaatttta tcgaaacgtt
    40381 ttgtgtaatg ctcactattt aagaaagatc tggtaaaaag catcgcagta ataaaagata
    40441 aattagcaac tatattgggc gctctatttg cgatttctgg agcattatcg aagagaaatt
    40501 gagcagcatt tccactgata ataaattgat acaaagcgac tgatgcaaca taatgaatat
    40561 agtagaggta agttttttct tgaatagata aatataagaa taaattataa aagaagataa
    40621 caatcattat cccataataa attcctagga aaacttgatc tcttacaacg tgagctgaaa
    40681 actcatctgg tgatctcaaa aataaagaga atttcattgg acctttattt tctaaacgaa
    40741 gaaaaatttt tgtttcctca ccccttttca ctggaattaa aaaaacgttt gtcctatatt
    40801 tcacttctct ctctttaaaa gggtggatgt ctcctaatac tttcttattg taaaaagctt
    40861 gtgaggaatc tgagaagtat aactcgatat tgtccattgt ggctaaattg cattccaaat
    40921 agagtctatc taattttgta gattcattct gaattgaaaa atgaatccaa taaacgcttt
    40981 tagtaaaacc aaaactaagt tctttttcgc cggtgctatt ccaagcagaa atagaattcg
    41041 taatactttc gcttatctta tttatatcgt aaattcgatt ttggtcttca aagtagagaa
    41101 tcgtatgcaa taggttttta ttttgaaatg cattatctag ttttagatta aactttaacg
    41161 tatcctctgc gaatacttgt gttacaaaga ggttatatgc aaacagataa taaataactt
    41221 ttttcattgc tgaactactt atttattaaa accatttgca acgtcaaaat ttttttattt
    41281 ttaataatta ttttgttttt ttttggtggt aagatcttct gaacgctaga taatcctatt
    41341 gatcttgtat gatatgaatt ttcattacaa tctctctgtt ttgatcaaaa ttaataaaaa
    41401 tatcaatata gcaactatcg ttttccattg atagagaata tataggctca taagctaatc
    41461 ggatcgaata tggctatact tctatcgaat tagataagct atttttcaaa ccgatccctc
    41521 tagaaccttc acgaaaagtt tttggtgtta aagaaaataa tttttagaat gggataaata
    41581 agagatttag gtatgagaga aaaagtttta gatacttctg aaaatattta cacaggaaaa
    41641 aatacatatt tattttttct agtttgtttg gtttttactc aatgtattca caataatcca
    41701 aacactaaat taatcgcaat tttaaactta aaatctcaaa cagttatttc ttactctaaa
    41761 ccaaccttcg aatgggtgag aggaactgcc attttagagg aaaaaccaag aattcagaga
    41821 ttaaatccaa tcaataatga tattaacttt caattctcca ttgatccaga actaccaaag
    41881 ggattggttt ttgatactgt caccggcact attagcggaa aacctgaatt agcccaaatc
    41941 cccactgaat acaaaattaa aatttctaat aattctgaat ctcaaacgat tcctttgagg
    42001 gttacggttt atggatattt acccctcaaa acgaaccaaa caacttgttc gaacaatgtt
    42061 ggcacagcga tttcatgcag tgggactgga caagatggtg agttgcaaat gggaagagag
    42121 gctgatatga atttaccgac agctcccagt gagtatccag aagattatat aactaaggat
    42181 aatgcaagcg gactcgtttg gaaaacttgt ccttatggaa gatctggagc tgattgtaat
    42241 acaggtagca caacgacagt tgattggagc aatgcacaat ctatttgcag ggatttaaat
    42301 aacgcgaata acggcaatgg tttcgctggt tacaagagct ggagaatgcc tactagagaa
    42361 gatcttattt caatggcaac tacaatgggt ttaagttctg ttgtttcaaa tacatatttc
    42421 ccaaattcga taggtcaatc ttggacttca aaagagttag atgacttgaa tgcaatcaga
    42481 gctcttggag ttagcttggt aatttcaact tccaaaagta atttaactcc aattcgatgc
    42541 gttacaggaa attataaccc aaaacctgct tatttagaca ataacgatgg aactatctcc
    42601 gactattcaa ctcgattgct ttggcagaaa tgtgctgttg gtttgagtgg agataattgt
    42661 gatataggaa gtgcaactgg aatgaatcga ggcaatgcac ttacagtttg taataatttg
    42721 agtttagtgg gaaagaaatg gaggcttcca aactataatg aacttttaac tttattaaaa
    42781 ccagatgaat ttagtaaccc cttggatcta tcgtattttc ctggaatcca attaaatagt
    42841 tattggtctt ctacaaatca tactggctca ccaatttcag gttattcagt taattttact
    42901 tctggaacaa gcgttggtgg attaaaaaca tctacaacaa cttctgtgcg ttgtgtggct
    42961 aatatggatt agacggtgtc tactgaaatg aatagtaaat gtaaagtgga tattaggaat
    43021 acagaaaaaa atgaaaaata ctttcaaaat gataacaaaa acaaattttt gctttgttat
    43081 cttattggaa aactttaaat gaaaatggaa aatttaaaaa ttaaagaaag cgaaaaaatt
    43141 ttttggaact tacatggaaa gatactgagc gatcagtatc gaccaaatga gattaagttt
    43201 tttctgaaaa ctgtggataa tcaatgtttt gtggaaatat tggagtattc acaaattcta
    43261 aatgaagatg gttttttaga gttattattt tactatctct ggctatttag agatgataga
    43321 gatataaatc taatcattaa ccaaaaaggt tttcctacaa aatatttaat taaatatatt
    43381 ttctcatcta tggggcgctg gattagccaa ggcaatgatg tagatgattt tttcaacgtt
    43441 gttggtttac aattttctag tgaaaaaagt ctcgaattac taacaaaaga aagggtggag
    43501 caaatagata ttagccttgg tttagtgttg atatgcaatc ttaaagaaag agacctaaat
    43561 agatttttat tcgagattca tgaaacagat tatattgtaa atttatttat agaagttttt
    43621 gatcaattag aagaagatag aatcaagggt ttctttataa agaacccgcc actatttgct
    43681 acacttgtaa acatattaaa agaattctca ataaaaaagg ggctttctaa gtttgaaaaa
    43741 ttcattaaac gatttcaagc agaagtggaa gtaattgaaa atttaattag tgccgcgaga
    43801 acaattcatt gtttttatga tgttcattca gagaaaaaga aaaatctatt tgaacgcgat
    43861 atgaaaagaa ttattcagat cgttatgatt ctagatgata caaaattttt acaggaaaaa
    43921 ttggaactac tcttctcgga atcagtgatt ttagattcag aagagattca tctcattgaa
    43981 aatattatta cgaatcccta tttaaaaagt atccttataa aaaaagaaga tttggaggat
    44041 tttgaaaatg aaccaagaga ataaaaaaat tctgccaatg caggcagaag ataaaacaca
    44101 aaatgaaaag attaaagaat taattgagat cgcagagaaa tttcgtatga ctaaaaagaa
    44161 aaatatagaa gagcaaactg ttattttaaa aaaagcggaa taagttggaa ttaaaaatga
    44221 taaatgaaca tagtgaatta actttttttc agggaatggg ttttgtagaa aaagaaatag
    44281 aatatacgat agagcgagat tccttgtatc gattgattct acacgattta aaaaatccag
    44341 ttcttggaat ttattcactt gctaggcatt tacaagaaca accaaatacg gatgttaaaa
    44401 aaatgcatca atacaatgga atgattttga gtgcttctga atcattatta tctctgattg
    44461 attcgtttca aatattcctt catgcagaaa cacaagatga acgaaatacc tttaaatata
    44521 ccaatttaaa tggactcatt tcatcagcta ttgcttctgt ttgtgctaac gctgagagga
    44581 aaaacattat tatccactgc aatctttcta gctttgacac tttctacggg agtgagtttt
    44641 tcacttctgt aattctacgc aatctattga ctaacgcgat taaatttacc ccgaaggatg
    44701 gaagtattct agttactact tccgtccgag gggccttttt tgaagtttct ataaaagact
    44761 caggtatagg aatgactcaa acggatattg gaaaattatt taatctaaaa aaaatattca
    44821 ctcgtctcgg cacagaaaaa gaaaaaggaa caggtctggg tttattcttt tgtagagagt
    44881 ttatttataa acaaaatgga acattttcca tcgaaagtgt tccagggctt ggcagtaatt
    44941 tcatttttac tctaccgctt atcagttaaa atattttaaa acggagttct agtatgaaca
    45001 aaaagaaaaa agtagttgta gtagcattgt tgatgacagt gggcaatata caatccattg
    45061 aaagatatga taaaaaacct tcaaaagatt tgaaagaatt agcaaatgag ttgaatcgct
    45121 ctccagatcc gtattcggaa aaagataaat ataaatcgtt gtgcgataaa gatgggtatc
    45181 cgttagttgg aaatactgcg agcaaaggag atattccccc tcgagtagaa aaaaaagttt
    45241 atttgaaagt tcaagaattt tgtaagacaa aataaaaatg gcatttcaag tttttaatcc
    45301 aactcaaatt caaaattttc cttgggaaaa aagacttcgg aagaaaaaag catggattta
    45361 cgaacttcca tttgatgaag aaatagaacc gacagatagt tctaaattgg tttggctccg
    45421 gactgcctat agcgagtatg caagcgcagc agcctttact aatatttcgc aagcgttatt
    45481 agcctgtgct gctccgttag atatggtttc ttgtgtaaat gattttgcga tagacgaatt
    45541 aatacatact gaggttgcaa tattagttgc aaataagctg ggttgtaatt atgcactaga
    45601 gattgattat aataaattag ttagaccggc atcgaaagag aatgcatcaa ttttgaaagc
    45661 atgtgaattg atagttcgaa gttgctgtgt tggagagacc cttactgtcc caattttaaa
    45721 aaaatcagca gaactcgcta agtcagagtt aatttcaaaa acactcacaa aaattcgaaa
    45781 ggacgaaatt tcgcatgctc aatttggttg gtatttttta gattgggcgg agccatttct
    45841 aaatcaatct gatcttaatt ttctgggtaa tgttacaaga gagactatcg attcctttca
    45901 aattgttttg aatgcggaat gctcagagag tggatatgga gttctagctt gtcaagattt
    45961 tgatcctatt tttctatctg ccgttcaaaa gaacgttata ctcccattaa aagaaagagg
    46021 aattatagta gagatagatt agaaaatcaa ataggtttat aaagaataat aatcaaaagc
    46081 aatctgggag tagaaaatta tgcatccata ctcttttgga tttttgaaat ttcgaattca
    46141 taggtatatc tatcaagaaa tataaaccta ttgaaataaa aaagggagaa tcttatgaaa
    46201 tttaaaagaa aagtattcag aaaaataagg aagttccttt tttgtttttt attgttatct
    46261 tcattccatt attgcaattg ctttttatat aaatctggta agttaaaagg aagtatggct
    46321 ctttccggat ctagtcaaag tggtggtgta ttaaacgaca ctacaactta ttcaattagc
    46381 ggtaatattt ctggtttaac cagtgaaggt cttgtgattg taaataatac cgaaagcctt
    46441 actgtatcag ctaacgctca attatttcag tttagtcaaa aactgaaaaa gggagatttg
    46501 tataaagttg aaattaaatc ttatccttcc tctccaccac aatcctgttt attagtaaat
    46561 gcttctggaa cagtgcagtc agcggatata tcaaatattg agattaattg tagcactggt
    46621 tacttagtaa gtggggtagt ttcaggacta aatagtagtc ttgttcttca gttgaatgga
    46681 ggaagtgatt tgacagtgat ggcgaatgga agtttttctc cctttgcaag tcccctagca
    46741 aatctaatga attattccgt cacagtaaaa acacatccga cagggcaggt atgctatgtt
    46801 tccaaggggg agggtgcaat tacatcgagt aatgtttcaa atattgaagt tcaatgcact
    46861 aacggaaatt tgccacccgg tcccttggtc ggtggttcta ttgtaaaaga attaattcca
    46921 caatatggaa atgataatcc atctttggga agtccatttg ttggcggaat tggtgtggca
    46981 aatgcgatag acggagcatc tacatccgca agatttagtg gtccttatca aattacaacg
    47041 gatggagttt atatttatgt ttctgatcga gataataatc gtattcgtag agtaagaaca
    47101 tccgatggtt ttgttgatac ctttgtcaat gatggaaata atatgataag tcagcctact
    47161 ggaattacga cagacggagt ggatgtgtat gtcgccactc aatctaaatg tcagattgtt
    47221 aaaataaaaa tttcgactgg tgcaatttca attataggag gaggggcagc atacacctgt
    47281 gccattttta atctcccatt agcaatgacc attgatacga ataatttata tattactagt
    47341 ttcggaaata ataaagtaat gcggttagat ttaagtaata actccatatc tacaattgtc
    47401 ggaaccgggg tatcaggtaa tacagataat tctataggga ctatcgcaac aatcaatgga
    47461 cctagaggaa tcataaaagt tggaaactat ctatttattt caactggtgg gcataatatt
    47521 cgaaaagttg atttgacaac aggaacctat tctacctcta ctttggcagg ttccactact
    47581 ggagtaaatg gtgcgataga cggtattggg actagcagtc gatttttttc accatttgca
    47641 atctcatctg atggaaccaa tctttatgtt gctgattccg gtaataaaac aattcgtaaa
    47701 attgtaattt cttctggatt agtgacaact ctcgcaggtt gcgattcaga tcctgctgga
    47761 actaccattg gaagtggaaa tccatctatc tcttgtgccg gtgctgcaag atatagagaa
    47821 ccatctggtc ttacttcaga tggtttttac ttatacgtat cagatcattt ggatcatatc
    47881 attcgaagag tagaatgaat gtaataattt tgaaaaagga aaatcaatgc ttggaaatat
    47941 actcctaact aaaaaatatc tgctttttgt ttgttactag cttgtttcgt aaagaaagta
    48001 gatgggatta aatattttaa ggtaaacaaa gaagacctta catttaattc aattacactc
    48061 atgcaaagaa atactgtatc ttcaaacgaa gaagaacaaa atattatttt ggaagagtta
    48121 caaataatta aagatagata ttctttagtt atggaagtaa aagacaatat tatcgaattg
    48181 tatgaagagg aatgcaaaaa aattaattca aatgtcgcaa atgaaattaa aaatattact
    48241 tttaaaacta agaaggggaa ttttccaatt actgaatttt caatgattcg attttcgaaa
    48301 gaattaaaaa ataactttcc atgtaaattt gaaaaagaaa accctgaaaa aaattgttat
    48361 aaaatatact atgaagaaat aagaatatga ttaaaccaaa aataaattta agtataattc
    48421 tgatttacct ctttctctct tttatgtatt ctgaatcttt tgccggagat ttaataccca
    48481 agtttgaaaa ggaagatttt caagtagaga attataattt ctttaataat aatcgctttg
    48541 atgctccagg aaatgaaaac ttttttcgtt tgtatttcca agaaaataag cttcgagttg
    48601 caatgtatcg ggatatatca tacaatagtg ccgatttgga atatgatgaa gagtatgtcg
    48661 cctactactc gatttgggga gaattagaat ttgcctattt ccgttattta aaaaacaaaa
    48721 atgtagatga cgaatattgt tttggaacaa ttaaaaaaaa taaaactgaa aattactgtg
    48781 attacaggac ggaagacaag ggagttttga taaaaaaaac tcagaatata aaaattaaaa
    48841 taccgagcac tgtaaataaa ttacaaactt tagtattaga gttaaaagaa aaatctaaac
    48901 aaagaaattt gttttcacac attacaaata taaatacaca attacgtcga cccaaaaatg
    48961 aggaagaagt aaacataatc caccataaag ttcccattaa agaaagtcct tctattgaaa
    49021 gtaaaactct gaaaattcta tatccatatc aaaaagtaaa attgatcgag gttattacgg
    49081 ataaaaatga aaagtgggca aaggtttttg tcactgacaa ttttaaagag gaaaatggcg
    49141 aacctctaga tgggtatgtg aatttagatt ttttatctct tatagatgag agtaaagata
    49201 gatttgaaag atcgatattg gaaaataaag attcctattg ggtaaaaaat tcatgttcaa
    49261 gaagtattcc atatagtata atggaagaat caaatttttt tagtaatcca aaagatggta
    49321 tattcataga aagtaaagat ataggaaaag atttagaaat acagattgag cataaaggtt
    49381 gtggtgaata ttggttaacc gtaaactata ttttgaagaa caaaaatata aaaatgattc
    49441 ctaatgatac attttggatc aatcaatatt taaatttact agttttaaca gataataaaa
    49501 ttttaattag taaggaaagc agaggattgc cttttgagat aaaggaagta ataaaggtta
    49561 tccgcaaaaa acaaattcaa aagaaaagaa taatttatgg aaggaattat cgtttttcag
    49621 acaatgaatt aggtcaatat tttagattct ataaaccaaa aactaaatta aaaggaatat
    49681 ttctaaaggt tgattttatt attaaccttt gatatgttta tgaaaatttt tttttaaaaa
    49741 ataatagtaa taaatttgac atgccccttt ctgtttcctg tgaattggtc aaaataccgt
    49801 ttggtgttgc ggcgtcccta tttctatttg ctggtgtact atgatgactg ccagcggcga
    49861 attacaacgt ttgtcttttt atcaaagccc tagtcatact cagagaagtt ctagcccaat
    49921 tctatttact ctttcaattc catttcttgt agtaggtgga attataggct ttcccgcttt
    49981 attggctgca cattatgaag agccaattta taagtttaga aggtaagaaa aaaaatggat
    50041 aaatatagat taacctcaac gatgccaaat ctttcaattt ctaaatcaat gtttgttaca
    50101 cctgcaaatg tttctttgcg tgttagagta ggtaatgaaa ttataaaaaa atagaggaga
    50161 taagatgaac catcctttaa aaaaaataat ttgtatgtta ttgattaata taatacattg
    50221 caaagaaaaa atagatcctc atagtgtaga caaagaggaa ataattcagg cacagtcttc
    50281 tagtaaaatg ggtcaaagaa aattaattga gggtcaatct tttgcggtag aattagaaga
    50341 cttcggaaaa gtaaaatttg ccagttacat tgatagcgaa gagaatagaa tgcagaatct
    50401 ttcttttgtg ctagaagata acggcaaaga ggtatataca tttccttttg tgatgggaga
    50461 ttccggacaa catttctttt tttatagtat caaggctatt tcctttctag atatggatgg
    50521 ggattttaaa aaagatgtaa cctatattat caagttattt acccggtgta ggaaaatgtt
    50581 taggctgtgt atttgatttt ggtggaatct atttttcgag agataaatct tttgaaatag
    50641 atttgaaaat caatgagttt ttaaatgctg cggattatag tgaaaaccca cctccatact
    50701 tgaaaattaa ggaagtgaaa aaaagattcg aaaaatttca atttaacagg tttacaggtc
    50761 aaaaagaatc acattaaaaa ataaaactat tattcgtaaa caagaagacg tattaaaaaa
    50821 tccaccgata caaatagttt tgtctccttt tccgatttga ttttttctat atccagttta
    50881 gatgtacaac gcatctttag ggttaatttg ttattattca gtttcaattt ctaaggtgaa
    50941 atttcgaatt gatactttaa attattgaag gttaaaaaaa tgaaatccaa atatttaaat
    51001 gcgtcttttt tataaaaaaa atagagcaat aaacataccc tagcgactaa ttgtcattaa
    51061 acgcaggatg gcaccttcct agtgcttact ttatcaagtc cccaataatc aaatatattt
    51121 cccatcctgc ctcaaggcag aaagtcaatc ggattcagat agaaaatttt cttccatcct
    51181 tctctttggg aatcaattct ccaccagtag gggattaaag taacaatagc ataatgtaaa
    51241 tggggaggtt ttccttttgc gttaccagtt gtgccaacag atccgattaa cgtccctttc
    51301 cttacaaaag aaaaggatga agtttttatt tcttttaaat gcgcataata atgaatatgt
    51361 aattttcttc caattaaaaa aaccgcattg cctccaagtt ctatctcgcc tgcataaagt
    51421 atcaatccat ttgtagatgc aaaaatggaa gttccttttt gtgcaaaaat atctacacct
    51481 ttatgtgttc ctgatattcc ccagggataa aaccaaaacg attttttgtt atagctattt
    51541 gcattccctc catcaactgg catgattctg ctttctggaa ttaaaaagga tacgatagta
    51601 agtagaataa aaaatcgaaa gataaatctc attgtattct ctgtcaatac tgctaatctc
    51661 atcacagtct tttaaattaa gtaataaaat tcgagtagca aagtctcttt catttactta
    51721 gttttagtat aatcccaata gaaatcaaaa tcgaaaagta gagacaagtt ttgaatttga
    51781 ttgaaaaaag gttttgtaaa tgagtagggg aaattctata tagccaggca aaaatttcct
    51841 tcattgcaat ggctagaata atagagaatg gtaatataag tagcaaaaaa agaattgcct
    51901 ctccttttat atgagcaagt aaaattacca gacacatcat aaatattttc tattccagaa
    51961 caaattcaat tcttcgattc tttttacttg cttcttcatc agataaattg actaacggtt
    52021 tggtcgaacc aagtcctttt gcaaatattt tattttgtgc tactcctttt tctattaaat
    52081 attttttaac tgattccgct cgacgttgag ataaaatcaa attattctct gaatttcctg
    52141 ttaaatctgt atgtcctaga agagttagtt tagtgaaatt ttcttttttt aatgcattgg
    52201 ctacttgatt taaaataggg taggattcag aaagaatttc agcagagttt agattaaaat
    52261 agattgaatt taaagatagt tttttatttt cagtcaagct tttcccaatt tcaaaactct
    52321 cttctttatt gcattctgct tcaatcgatc tagctgtgta gatccctcca acaagataat
    52381 gaacaataaa atcgaatatt gttcttttaa ttacaattgg tttgattcca ttttggcagg
    52441 aaacttctct tggactagat gcttcaaatt ttccccaaat cagggttcca tatttctgtg
    52501 atatttcttt gtctggttgc ggataataac gcttattctc tatatcaatc acaagtatat
    52561 ggcaatttaa aaataaaaag agaaagctaa ttataaatat agttccagat ggtataaatt
    52621 taattagtag catgtgattc gaacagtcct tggtgagtag ataaaagctg ttaactgctc
    52681 aaacaacgca tcttcaaatg tataatattg aagtatttca tagatgccag agtctggaca
    52741 gtattttgat tcttggattt tgtattctct tggtagaagt cctcctagaa aataggtttg
    52801 atgaatttcc tttgtagttt tttcggatgt ttttattttc tctaaaaatt cgtgtttgtc
    52861 tttttcgcat tgtttatgat tttccatttg gcaagatttt ggaagaggag gagccactct
    52921 aagtcttgca tgttggcaat ggattaaatg aaaaaacaag gaaagaaata caacaagttt
    52981 cgattgtttc attctattgc tccacacagt agactccaac acctgtatta caggtctgtg
    53041 aattgcctgc atcggaaaag gaaagagtat ttgtgaaatt cccatttgct ccataaaccg
    53101 aaacagccgc ggaacttgtc caattggagc aattactagc acttggtgtc caagatgtat
    53161 cgctatttac taaaatacct gtatatatat tggaaaaagc gcctacaacc gagtttgtta
    53221 aatttccacc cgatactgca aaaacagaag aagcgttagt agttccaatg atagtagttc
    53281 catcttgtct tctatattgt ttatttggtt ttaataccca attggttgtt tgatttcgaa
    53341 tagttgttcc acctgtcgtc ataagaattg ctctataatt ggaaggaggc aagccaatca
    53401 tgattgctcc attgttgcat tctgtatcta ttcccacaat cccgtcgcca aatgcacccg
    53461 ggatagagcc aaaatccgca gctggagaag ctggggcatg ggcgaatata cggcagaagg
    53521 cggccgcact gcaagaagtt gcatacacat ttactgtata gttttgggaa ctcccatctt
    53581 gtgcatagac tgtataggtc attgtgtagg aaaagttgtt tgaggtaaca ccacttgttt
    53641 gcgtgactgc tccaattttt actgaggaac cgttatgtgt aaagtttgcg attagatttt
    53701 tcaaattcgt atttcctgct acagttacag ttatgtttgt tcctgagatt gcaccggttg
    53761 catttggtgt ttgtatgcca aaggataaaa tatctttagt gcttgcgagt gaagttgggg
    53821 tatttgtctc gctcgttgtg gttactcctg agttgcctct agaattttga ttcggtctta
    53881 taaagagtaa tgagtaaatt gaattcgttt tattatctcc tgtttgaagg gatgtagcta
    53941 cttcatgttg taggagaggc catcctgtgc aattgcagta taatagtaag ataaaaagta
    54001 ttaagtgttt ttttgtattt ttcatatgcg ttattctttc ccaaccaata tccagattta
    54061 ttttttcaaa aaaaatgaaa ttgaaattgg tttggttatt ttctaatttc ttaatttgtt
    54121 aaacgcaagt aatttcagtt aaaatgatac tatgctaatt cgaaaataaa acacttctaa
    54181 ttcgatggaa gtattcaatg ctactatcga attagtcttc ataaaatctt tgtattaagt
    54241 tcacttaaag atgtttcaag ggataaaaga agaaagatcg acctagataa aaacctttcg
    54301 aaatttttag aggaaagaaa taaaagagta atccagatac ttgaattagc tagtaaaagt
    54361 cagtaatatt caatacaaat agaatctttt ggaacttgta ggtagagata tcttaaagaa
    54421 ttattaataa cttaccttat attatcattg ctgatgcaag ttacaagaat cacttctctt
    54481 tgatagcagc aataataata ctaaaatctc cctctagttc ttctaagttt ttctctggaa
    54541 tgaatgcttt tgaaacaaat ccatccagat aaagagcatt taaacattct cggtcttcaa
    54601 aaaactttgc aaattggtat aagttgattt tatttttaga aatggcaaat atcaatttgc
    54661 cgctatctaa tatacaaact ccatttcgaa tatgataatt ctttgaatct tgatgaaatc
    54721 ctttgtggat ttttttattt attattagca taggtccaga ttgggttgca tactcaattg
    54781 tttcatcgta ggtaaaggta gaggaaggaa caatatttgc ttttttattt ttagttagat
    54841 agaaaacacc gttagggttc atataaaaat tcccataacc ggattttaga ttgatagctg
    54901 tttccatttt tccagattca atatagagtc caagaggatt taagtcttct ttaaagatac
    54961 ctccattcat tgcaaagatt agagtattgt ttaaatggtt ttttagtgat ccaatatttc
    55021 gaaagtgatt tccgttttca tctttactaa aaaattttat atcaactagg ttgggatcaa
    55081 tctcggtata aacaatctca ggataattga attcatgtaa ataataagtc ccacttacta
    55141 aaagaaaaat aaagataata ataagttttg tttttattct catactatag ttaataaata
    55201 gaatattttt attttgcaaa attttttaaa aaaataaatt tattttaaat cgaatcatga
    55261 acttaaaata ttcatccttc ctcaaaatat acgagtaata aattaatctc atttcttatg
    55321 aaactgttaa acgaatagta aagattcgaa tcattgttaa acatactatc tccattgtga
    55381 tacgttttcc tataaataaa aaccgagtga gtaaaaaaat cataacaaca ctcaaaaata
    55441 attattttag aggacgatag tttctacatt tataaactgc agcaacatat tatttcaatt
    55501 caatactttg cagaattggc ttccaatgat ttttagaaaa atcaggattt ccttcttctt
    55561 gtaattgaat cgatataaat tttccattct tccattttac ttttgcacgg cttacccata
    55621 ttttttttat aagtttatta ttttctaata attgcaactc tacctgactt tcatttggta
    55681 gtgtgtcttt ttttttgaga attgggatga ttttcgcttt atactgatct ccatatccat
    55741 aaacatcttt ataatctttt ttgaattctt cgcgagagat agataagata atttttgaat
    55801 ccattttata atgtttgtca taaaattcat gggttttgta atcttcataa taattaggga
    55861 gttctaaaca tatttcgcca ccacataata ttttatttcc tgatttaggt ataataacgt
    55921 ctctttgtcg gcttaaggtg tttagaattt gattccatag cttatcattc ctttttttgg
    55981 gagtatacct aattttccca attgatgaat tcaatcgaaa agaggcaaaa taaaaaaatt
    56041 catcctttga tttagggaaa gatatcatca gagtataaaa ttcgaaatta gaacccttaa
    56101 agtttttggt ttcttctaat ttaatttctg gtgaatttgt gaatggtttc atagtagttt
    56161 ttgtaaatat ctcaggtgta actgattctg ggaaatcttc aaattctata tgtatttctc
    56221 ctttttcatc ctctgtttca tttgaatcct tggttccaaa aaaaatagaa tgctctgcaa
    56281 ttaacgtatc tggaagggat aggtagagat gatttaaatc ccatttgata tatttattct
    56341 ccatgtgctg agaatataaa ttagaattta aaaaaaatgc aaatgaaaaa atgaaggtaa
    56401 atagaaacat atattcgtta ctcctttttt tatttatata actcacctta cattatcatt
    56461 gcttaacaaa agcaatgatt gagtttgggt gaaccaaacg ttcaaatgaa gtttgcgcct
    56521 agcggcgggc tttcctccca tcgcttttcg ctccccatca gccgaagcta taaagcaggc
    56581 tattgaaatt attagtttat tagctttatc taatattcta atttccttta tcatactgat
    56641 caatttctat cttctgtgta aggtgagtta ataagtcttt ttataaattg tgcaactcaa
    56701 atagttcggg atgctgctgg aacgaaaaga tatgaggata taattcaagt tccacagaag
    56761 gtaatcaaat tcattttagt cgaaaattct gtttacatgg ttgtaaatgt gaaagaaaaa
    56821 aagtcatacg agaataaagt aagagtggaa gagcgatgtt atcttgtgca aaactttgag
    56881 agacttgtta ttactcttga tgttgctgat tatattcatt gtccaaagat ttataatcaa
    56941 ttatatgatg ttcaggaaat cctatcaaag aattacaagg caatgttatt tattcaagaa
    57001 aagattgcta ttgtttctca aaaatgtccg cacaaaaatt ctgcgaccga aaattttaat
    57061 aattccgtaa aagaaaattt agatttttta tctgccaatg ttccaaacta tgtagaaaaa
    57121 tttgcaaata gactaccgga tgaaatggaa aaaattgcag caaatcctac acattatccg
    57181 ctagaacctc tacttgacag tgaacaacaa gaatatcgtt atagcaaatt tttgtagttc
    57241 tggaaaatat ttttccaagt atccaaacga attatatatt ttttggatct ttttcatata
    57301 aaagaagata gcaagaaaat taaaaaactg agaaattaaa aattttttac tttttattag
    57361 aagatgatac ttgaaacatc gcttctaatt ctaattttat actcgtatca aactctggaa
    57421 cgatagtctc tgtctcgcta taagttttta tttcagaata tctttcctct tttggttttt
    57481 gatacacttc aactgtatta ttatttaaat taaaaagcca gtattcagga atttttgctt
    57541 ctgcataaat gttcgccatt tctctatctt ctgcgagagt agaaagggaa acttcaatca
    57601 caagttctgc tgtagtagga tgcgattcag aatagtcctc cagatttccg gatatgatag
    57661 aaatattttg ctcaggttcc gaccgagtga gtgtgagcgg ttgctccgat cgtaaatgca
    57721 actttttgga aattttatca ctgaaaaaat tcattattcg taatatcatt gttgcgtgct
    57781 tgggtgattt aggtatcttc tgtattacaa ctccctcaat taattccgtt cttttagaga
    57841 gaaatcccat ttctccgaga gcatggtatt ctcgaataga gattaggtgc gttctgcttt
    57901 ttatgcttgg taaatccaaa attgcgctca tacgagtagt tttacaaaaa gtttcctaga
    57961 ttgtcaaaac cactttttct aaacgatttc gtttttaaaa aagtcttgca ggactataat
    58021 tgagaattag gattaagagc aggagagggt attacaatgg actatcagca aaaaggtttt
    58081 gaaaaattaa aacaagggct tggaacttcg ctcgtttttc ttgcgattct ttggggaatt
    58141 gtgatttcgg attttattct tccaatggat tttacaacgt taggtgtata tcctcgaagt
    58201 ttgtttggtt taattggaat accacttgct gtatttttac attctggttt ttctcattta
    58261 gcatctaatt cgcttccact cttagttctg atgacaacaa ttctgatttt ttatcccaag
    58321 gttgcatttc cttctatgtt ttttatggta atctatacaa atattttagt ttggatcttt
    58381 gggagatctg cttatcatgt gggggcgagt ggtcttgtct attcactggt tgcattttta
    58441 attgcagcag gatattataa gaagcgacct ctttttgcgt tagttgcact tgtaattgga
    58501 tttttatatg gaggacttgt ttggggagtc ttcccaggct ttgtaggttg gtatgtttcc
    58561 tgggagtctc atttgtttgg agcgatcgtc ggagtttatt tagctcattt ttataaaaaa
    58621 gatttataaa tctaaaggct aatcggtatt agacgacagt gatttcttgc acaccccaaa
    58681 catcttgtcc gctgatatcg agtaaaactt ttatataatg tttgattgca gaagaggtga
    58741 ttaaattgtc ctttggaatt ttcagctgaa aaggaactgt gattacttct ttaggctcta
    58801 gcacataatc atttaaactt acaattccaa ctttttcatc tgattctttg gttttatttt
    58861 gatctttcaa ctcttcaaca gttaaaatat ataaaataat tttctcaatt gtgactggat
    58921 attctccacc tgtaaatgtc gcgattccct ttacactttc ttgcattgaa gccttgtttt
    58981 tgaaaagttt aacttcaact gttggtgaac cggaaccaac catacttttt agtgaatcta
    59041 taaatcccat attcatctcc tcaatctaaa atcaatctaa ctaattctga actttcttca
    59101 aagcctttta aatttgaagc aaaaatttct gtttgaattt tttcttttac tagaaggctt
    59161 ttaacatcat tgtcttcata cattacttta gtaattacaa tatcgccacc cttgcatttt
    59221 ccttccacgc gagcagcaag attgactgtt ttcccaaaat aatctagctt ttcattttga
    59281 tttaccgcga ggactggacc ggaatggatt cctactttca atacaatcac attttccccg
    59341 tattcttttt tgtttaactc tcttaattct ctttggattt ggagagcgta ggaaacagca
    59401 ttgacaggta ataaaaacac acccataata gcgtcgccta tcgtttttac aatcgctcca
    59461 tcaaattttg aaatatttcg aattaaaata tcaaaatgac tactcacagc tttataggca
    59521 aaggcatcgc ctcttgtatt ataaaactct gttgagcctt tcaaatctgt aaataaaaca
    59581 gatagattct taatcccaat ttcctctcct ggtcttaaca cttgagaaga aaataattct
    59641 ctgaattcct gctttgccgt tacttctgct gctgttacaa tattttctaa ccaaactgca
    59701 tgttctaact taatggtaat ttcttcctta ggatttgggt tggtgagtag aattcttttc
    59761 tttccaagag aagtttttac ttcttcattt tttgctccag tataatgaat cgtatctatc
    59821 ttatcacctg tttcactgac ttggatttgg agagtatagt tattttgcaa actatagatt
    59881 ttatagattc ctgggaggag tgaatattca atttcttctg tttgatttgg tgcaactcga
    59941 atttgagatt ttaaatgagg ggtgcttcct ggacctgacg cacagtagat tccggaaact
    60001 acttttcgaa tagaaggatt tggtgtaaac gttagttcta cagatttatc gaaatcggca
    60061 ttgtagtcaa tattacagga agaacagtga actttttttt ccatttccgc tagagtcttg
    60121 taacttgatt ttgctccacg acaagctgga caaagtagat cccagtttaa atcaaaaaat
    60181 ccttcttttg ttcctcgaag gaaaaagtct aacacatcct ttttgtcttc atgccaaatt
    60241 gctgcgagtc tgtaaggtcg aatttttact aaatcgattt cttgtgactt taaaataaag
    60301 gaggcaacta gattggataa agtatcgtta tgcgatattg tagcaaattt atgaagtagg
    60361 ggagataggt catgaaattc tagattgtgt ttgatttctg taattccaat attgtatgta
    60421 ttctctttcg aatgttcaat ttgtctgtag taatttttga atttaggaat tacatcaaag
    60481 ttaaatttta gatacataat aggtgtgagc aatttaaaac gtaaaatcac ccgcatagta
    60541 gtttgaatca atgtatgctc tctatcaatt tcttttaatt ccagtctatg tatgaatcga
    60601 atgaagggac cttttgtata ttctcttacg atttggaaaa atttaccctc tacccattcc
    60661 gatgattttt cttcgaactc aaaatggaat ccggtatttt tttgggaacc ttgtttcttg
    60721 cttccgcctt gtggtagggg aaaatccctg taagccattg ccggctcact tagaaactga
    60781 tttgctctat cagtgtctga tacatattgc caaacttttc caagattcgc tttcacttct
    60841 atttcgaaag taaagaaaga ctcatttgat tgggtttcgt agtgcatgca tacgaactat
    60901 aatcaatgaa attaaaagta aactctttat ttgccctatg tagtattgga gggaatagaa
    60961 ttgtaatatt aaaacaattt tttctcttgc taaaaataaa agagtaaata agatataatt
    61021 ttgtaaattc attgagagtc tacattcaat acattcagga gaaaagtatg aaaataaaaa
    61081 ttataaaaat gttttccctg ctcttaattc cagttttatt tttgatggaa tgtaagagta
    61141 aatcaaatga cgataataag actaacttac ttgtattagc tgctcttgca aatagaagta
    61201 gtggatcggc tgctagagat atgtatacag gctttgctga tatcccagct agtatttata
    61261 agactgttgc atctggttct gcaagtagtt ctattcaagc agaatctaga actctttata
    61321 ctgcagatta tcgttttcaa aatagcacaa atactgcttc cgtatccggg gtttatgatt
    61381 tagtaagagc aactgcaaag accaccagag atattgcgaa gtcgataggc gatttggttg
    61441 gtagtttgga gacaattcca gtaaccacta cattgactgg gacagcaact tgggcaaacg
    61501 tggcttctaa gtatcgttat gcgccaagca caattttaac aaatggtaaa aaactagaag
    61561 tttggtggaa taacgcagca gccccttatt ccaataacaa agcaatcgaa ttaaattaca
    61621 ctgggagcac tgcttctgga aatatgagtg gttttgtttt tgttagattc ttgcaaacat
    61681 ccacttcaac aactctttct aaggcgtata ttaattttaa ctacaacgct tctactggaa
    61741 cgagggtaat ggttgttatc cttcaagata tcaacggagg aggaactgga acaaatttta
    61801 gggatagagc acatttctat gtgcaagaag tgaatggagt tactacaatg gatggaactt
    61861 ataccataac tggattcaat ccgaattcga ctggagtaaa tgcatcggat agagcttatg
    61921 ttttctctgc gattggaaat tctagtagag ctgttgtgaa tgcggctttt ccacaagtaa
    61981 cagataatac aacagctatt tatgcaacca caactctcgg aaatattgga caagtttgga
    62041 ccaattttat tttggcaaat acttccattg tatcaggcct taatacaata ggtggttctt
    62101 gtagcgcaag taatattact acaactagtg cgtcaggcgg aaatccgggc actcttatag
    62161 caaccaactc aattgcaaac ttaaagactt gtttggattc aattaatcca gcagtaaatg
    62221 ctaaagacgt ttatttctta acgaatatca aaaaccctgc ttacttctct gtatcaggaa
    62281 ataccgttag tctctatggg gtagaaagtt tagacgcaag tgatgcaaat aaatcagctt
    62341 ttgatgcctt acaaaatgct acttctttta ctccgatttc ctctgcggtg agaaccacat
    62401 ccaatactac ttatgcagca gcatttgatg cgataactgt ttatagttta aacctattta
    62461 caggaacagg agttctagca ggaacaggag ttctagtcgg atctagtgca actagcttaa
    62521 caagcctaaa tgcacagtgg ggaaatggaa caccaggaac aggaacttct agcgcaactg
    62581 ctggaacaaa ttctgtgaac ggtagctcgg ataacacagc tccgttttaa ggacgacaaa
    62641 aggcgaggca gcgttgccta acactgtcaa cctagggatt tttaagccca tagcttggct
    62701 atggtgagcg tcaagctatg ggcgggaatg cacgaagact cgcgaagtga ataaaaaata
    62761 aaccttctct tgtcttcgtg ttctctatgc tcttcgtggt taaatctttt tgatttctct
    62821 taagttgaaa gcattgggcg tctcgcctac atgccttgct ttcagatcaa ttcagaaatt
    62881 ttctttgcaa tatctttcgg ttctaagcca tcaactggca aataaaaatc agaagctgct
    62941 ttataaaaag gaattcttct ttctaaaact tctctgtaag aattaatttc actgagctgg
    63001 ggtctctctg tatcattaat aactttttct accagataat caatgttatt cgaaagacct
    63061 atgactgtgc agaaggattt tagaagattg gttttctttt cgctgtagaa ttcttttccg
    63121 tcacgatcta catcaaataa aattccaccg ccacaatcta ggatgatatt tcttgattta
    63181 gaaagattct ctaaaatctt aaattctaat tttctaaagt tattccattt tccattttct
    63241 tttttaattt gctctgcaat ggaaattccg cctgcttcat aaactgcaat catatcagtg
    63301 gatacgattg gcatggataa taatttagat aacttgcggg atacttttga tttgcccaca
    63361 cctcgtggac cgatgagtgc tatattcata ttgcttgtaa atcgacaatt gacttttgta
    63421 attctggaat tgtgatgtag taggactcaa tgtcaccttc tgtgattcct aaaattttta
    63481 tagcgttagg ggaaagctca tgagaaattc cacctatatc aattccaatt ccagagataa
    63541 ctgctatttg atttgcaaca tggactacag aaacaagctt ttgaaaattc ttggcttggt
    63601 ctggattatg gtggtaagcc actacttctt ttagttcttc tggaaagttc catgtttgta
    63661 gagctttcat tccaacttct tggtgatcat aaccaaaata ctttctttca atttccgtaa
    63721 agctaagttt ggaagttttt agttcttgtt tgatttctaa aatcttagaa ggaaagaact
    63781 gagctaaaat tactttgcct atgtcatgta ataaaccaga agtaaaagct agatcttttg
    63841 gaattcctgt ctttttttga attgcaatac gtgcagaaag ctctgcaaca actaatgagt
    63901 gaagccacat agactctgct tctagttggt agccaggtaa atctttattt agaatttttt
    63961 tagatgcagt gagtaaaata atttctttaa ccgtttttat accaagagtc ataagagatt
    64021 cttgcacagt gcgaatgggt ttacttgcac gatagtaagc agagttagaa agtttaatga
    64081 tagatgcagt gatggctgga tcttttgaaa tttcattggc taaatcatga atggatacag
    64141 cggggtcttg gataagcgtc attacccgca tgacaacatt ggacatggga ggaagtttat
    64201 ttatatctgc cagaataatg tcaatttgat ctttcataga gttatcttac cttataaaga
    64261 tatttttcaa tgcctgcttt ttttagtagt attcgaccat catccaaata caaactaata
    64321 gtcctgcctt cgtgaccacc tacatcttct gcgatgattg gaattttttt ttcagcaaaa
    64381 aattcacgca caatggcaat gttgctttct ccgatattgt tcaaaaatgc agaggtaata
    64441 ttcttaaaca tagatgcagc tccgaatatc ctagcataat aggttcctgg tttacaacct
    64501 tcgtcttcga attgcttgat taatttaggt aaagcggttt ctccatactt ccctggattc
    64561 tttagtttat cacgacccat tgtatcttta gcgagcatga tatgagaaat ggctccgatt
    64621 tttagatcgg gttgatacaa tactacacca atgcaggatc cgagagtggt tcttaagatg
    64681 ttaggagact tgccaacagc aatttctgca atcccaacat ttatgatttt agaatcttta
    64741 ctcaacatcg tttcaaatat tagaaaaaca atacttgtct ctactgtcaa gtgagtaatt
    64801 cgaattgaat gcaaaatact tgctttactt agaaggaatc ggtaagagag tagattacaa
    64861 ggaatcaatt ataattggag taattcttgg atacaattaa agaagaagca atcagcaaag
    64921 aaccagttcg gaaaattaaa atcaatcaat cagaagtcac tattcttgga acagctcata
    64981 tcagcgtaaa aagcgtagaa gctgttagaa atataatcga tcaagaaaaa ccagatgtgg
    65041 tttgtgttga actttgcaag tcaagaatga cctcgattca ggatccagag cattggaaga
    65101 aattagatat ttttaaagta ttcaaagaaa gaaaaatgta tttgctactt gccagcttaa
    65161 tcttgtcttc tttccaaaaa aaattagggc agggagagat caagccaggg gatgaattaa
    65221 aagctgctat cgatgaggga aataaaattg gagccaaagt agttcctgtc gatcgggaaa
    65281 ttcagactac actcaaacgc tcttggggca atgtaggact tttttcaaag atgtatttac
    65341 tctccgcact tatcacatct cttctagtaa aagaggaagt aagcccagaa aaaatcgaag
    65401 aaatgaaaag tgatgatgca ctaaaagatc tattttcaca actcccacct agatacaact
    65461 ccatcaaaga agtgatcatc gatgagagag acaaatacct agcggaaaat attcgacgac
    65521 aggcgagaga ttccaaaaaa gtatttgctg ttgtaggagc gggacatttg gagggaatca
    65581 tgaagcatat tgtagaagat caaaccattg atcatctaga tgagattcct aaatctggaa
    65641 tttgggataa attacaatta gctattcttc ctctttttat tttatcgtta atggcggcaa
    65701 ctttttactt cagtggattc gaggcaggaa cagaattagt aaaagaatgg attattgtaa
    65761 aaggaacgtt agctgctctt ggagcactca tcgcattagc tcatcctatt tcaattttac
    65821 tcgcatttat tgcttccccc attggaaatt ttaatcctat tattaaacct ggttggcttg
    65881 cggcgttaac cgaaatatgg atgaaaaaac ctctggtaga agattttgaa aagattgcaa
    65941 ctgactctga gcattttact ggtttctgga aaaatcgggt cttgaaaata tttttagttt
    66001 tactcctgcc acaaattgga agtagcattg gaactttcct cgtaacttgg aaaggaatta
    66061 aaggcttttt aggataagtt ttgtatgcta aaacttgcaa cttctgaaca gagagatttc
    66121 tatgagaaaa aactctatcc tatgcaagat aaaattttta gcttattaca atcagataaa
    66181 ttttatctga caggtgggac ttgcctttct agattttatt ataatcatag atattctgat
    66241 gatttagatt tcttctttat gggaacggat tttagaagag aagattttga agctgagttt
    66301 cgtgaaattc aaaaaagaat tcatctaagt tttaactcta aattggaaat cagttcagac
    66361 tattttaaaa ggcttactgt ttttgaaaat gaacagacac tcaaagtaga atttatttat
    66421 gaaaactttc aaatggttca aaaacccatt cgaaatgatt taatatttct tgatacaaag
    66481 gaaaatatat ttgggaataa attaacagca attcatggaa gaaaaacgac aaaagactac
    66541 tttgatcttt tttttctttt gaaagaatta aagctttcag atggaattaa atggtctgaa
    66601 tttaaacatg ctccagtcga ttatgaaggt gcagttctct cattggttgg gggacaattg
    66661 gaaggagatg tattcatgat gcaagaaata aaactagacg atttcaacga attccaaaac
    66721 aatttaatta cagagttact atcgtatgca aaaacggttt cataaatact tctgggatgg
    66781 attagagaat tggagtccag agtttcaact tcgtcgaata attgaatatg cgagtttccc
    66841 agatttaatt caatatccat ttgaagaagt aaaagaaaac ttatcaaaaa ttcgcctaga
    66901 gaaacttaga acaggggaaa atagaaaaga attgattatt aaattatcgc cttttgtttc
    66961 tcaggcatct tcttgggaag aggcaatttg gaaaatgatt gaatactatc gagaacaaaa
    67021 caaagagagg atagcctaag atgttagact ttgtagataa atttaaaagt gaattactcg
    67081 gaatccagaa tacgttaaac gagctacttg gaatcaaaac attcattgat tcgcttaaga
    67141 aaacagccca gttttttgac ttgtttttct cgattgtgcc atttgaagta attttacttc
    67201 tcatattttg tgctttgttt ttggttctag taaataacat ttctccaacc acaccgcgta
    67261 ttaatattac aatcgctgtg gggctattct gtgttatctc cttgtatata aatcatatct
    67321 tcacaggtga atataaattc ttacatgtat tttatatttc tctttatgtg cttgttcctg
    67381 cttattctgt tgagttatct aaatttgctt ataaacagat atataatata agacaaaaat
    67441 ctatgaagat tgaaaatcct gaagaattag taaaatcaat acgagagatc aaccaaagat
    67501 attatgaatt tttaaatgca caagcagatt ttaaaaacaa cccattagat ctaaaaaaat
    67561 ctgcagctaa tttgaaatct tccattgaac aattagaaaa aagaatatga gtcgtagacg
    67621 ttatattcta tatgctctag cttatattac aattcctgtt ctgactaata tggttatagc
    67681 agcgtttagc tataccgttt tagtatcctt ttttatgacg agagaggaat ttcaagttgt
    67741 aagtcaggta ggggagtctg gagatgtaac ttctttgatt ttttcttacg catcttttgt
    67801 gattccaaca gcaattggct atgcttataa tttaccctta atcttttata tgttcaaaaa
    67861 agaaagacca ccaatgtcga taaagattca gtcgattatt gtaaatctgc caattgtaaa
    67921 tgcgacagtt agttttgctg gttggggact tgcgttatgt ggaacattta cttattttta
    67981 taattatgat gtgtatatta gtctccaagc atctcttaaa ctaatctgtt ttaatatttt
    68041 aatggcaaac ctttgctttg tgtttgtata ttatctagtt gatatagtaa ataaacgttt
    68101 tatcttattg gtattaaagg gtcagaatct tagttcgatt ccaaaaacag ttaagatatc
    68161 tatcagagga agatttttta tttttttcct gacagttgcg gttactccgt gtttgctttt
    68221 aattggactg attttatcta ttctttcaga aaatcaaatt tctagagatt atacccatgt
    68281 cggttatatt tttataggga tcattctaac tggggtcata ttgactcttt ttatttccaa
    68341 ctcatttgca aatccaattc tagatttaca aaaagcaacg gaaaagttac aaagtggcga
    68401 cttagattca agagtgtctg tttatacaaa tgacgagatt ggttttttag gtgagaattt
    68461 caatcatatg tcctcttcca tcaaagaaaa agaatttata aaagacactt ttggaaaact
    68521 agtcgatccg acagttcgag attttctact cgaaggaaat atgacgttag gtggtgaaac
    68581 aaaagatgta acagtcttgt ttgttgatat tcgtggattt acttccattt ccgaaaaatt
    68641 gccgccgaag ttaattgtaa actggctcaa tcaatatttt gaacaaatga gcaattcaat
    68701 ttcaaaagaa aaaggtcttg ttaataagtt tataggagat gcaattcttg ctgtttttgg
    68761 aactccaatt cctctcgaaa atcatgcaga ttctgctctt agggctgctt tggatatgcg
    68821 caatcaagta aaacttcttt ctgaaaagtt ttcaaaagaa ggacttcctg aaataaaaat
    68881 tggaattgga attcattccg gaaaagttct cgcgggaaat attggatcga tgaatcgact
    68941 cgagtataca gtaattggag atacagtaaa tttagcttct cgtttagaat cttattgtaa
    69001 aactgcaaat cgagatttgc ttttaacaga ggcaacgaaa gaattgattc aaggaaattt
    69061 ttcttttgaa ttcattgata aaataaaagt ccgaggcaga gaaagcgaga ttagtatttt
    69121 ttctgcttga attaatcatt tctttacata acttgaacgc gattgaggtt tcttcatgaa
    69181 agataaaaag aaaatacttt ggttttttat tctaacttta attccacttc tattgatgtt
    69241 gctttttcgt ttcagtggta taacggaacg agcagagaat atgctaatcg attttaggca
    69301 tcaatacttt aatccaaacc ataaattttc tgataaaatt attttaatcg attttgatga
    69361 agacacattt aaaaaattag gaaacaaaaa agagtttggt agatggcctt ggaaacgatc
    69421 cgcctatact cctattttag aatatttatc tttaggtgcg cctaagatgg ttcttttcga
    69481 tataatgttt tttgaacgct cagaagagtc ccaagatatt gcgttaggtg atatatccga
    69541 tagattaacc caaatgggta ttcctgtttc tcatgctgct agtttcagat atgaagaaaa
    69601 gacaaatgca aaaccagaag atattccaga agatatgaga gcagctctcg atacaatctc
    69661 aatgaaattt ccatccgaat cagattcaat tttgagaaag tataatattt tttcttatcc
    69721 aacagatgat attatgcgta aaattagaca tcttcattcc gtttcctata aatcggatgt
    69781 ggatggagtc tcacgcagaa ttgaaccgtt ttttaaatat gagaaactcc atttaccagc
    69841 acttccattt aaagggtttg atgcggtcaa gccaatggat caaattgaca tcagtgaaaa
    69901 gtcacttaat ttaaggtcag gggatgaaaa tacaaatatt ccattggaaa atggtcgtat
    69961 gcggcttcat tattattcac ctgaacaaat ggagaatatc cctcgttttc ctgtctggtc
    70021 tttaattgaa tcgtatgaga aagtaaaaaa aggggaagcg gatcctgcaa acttagagac
    70081 aaacttcttt gattttcaaa ataggattat aatcatagga acgtcagccg ctgcaacctt
    70141 tgacgataaa gtaacgcctt atggaaaaat gcctggattt gttttgcatg cagtcgtatt
    70201 atctaatcta ttagaaaagc attttttaaa gctatctcct ttatggttta acgttctttt
    70261 attagttatt acaatcccaa cctgtgtatt tttctctttc tttttccaac aaatggctct
    70321 tcgcgtattt gttcctgctg ggatattttt gggctatacg cttcttgcgc tctttgcttt
    70381 caagtttgat attcatttgc cattagcttc ttttttaact gcctatccgc ttgcctttct
    70441 tggttctctc ggatacttat cttttacaga aggagcagag cggagaaaat acagtaaggt
    70501 tctatctaac atggtggatc catctatcgt cagtgaagcg ttaaacgatc tagaagcgct
    70561 taaaaaaggt ggagaaaggg aaatcactgc attcttttcg gatgtggctg gattctccac
    70621 tattagcgag caattgagtt ctgctgattt agctgcactt ctcaatgaat atctttctgc
    70681 tatgacaatc atactaaaac acaatcatgg gacattggat aaatatattg gcgatgctgt
    70741 ggttggaatt tttggtgcgc cgattcatag agacgaacac tttatagaag cggcaaaagc
    70801 ttctcttgaa atgattgaaa agttaaaaga actgaaagct tactggagtg caaataatct
    70861 ctattgtaag gatgctcagg atatggatgt tcgaatagga ttaaataccg gtcttgcaaa
    70921 agttggattc atgggaaccg atgcgttagc ttcctatact atgatggggg atactgttaa
    70981 cttagccgct cgactcgaag cagcaggcaa agattatgga gtgaatattt taatcagtga
    71041 gtttacaaat gataaaatca aagagcaaat gtttacccgt aagctcgata gggtgcgagt
    71101 aaaagggaaa aatgagccag ttcaattatt tgaattgatt gccatcaaag gacaagaatc
    71161 tgaaagtaga agggagtcgg ctcaaattta cgaagaggct tttggactct acttaaaaca
    71221 agagtgggac aaggcaattg agttatttga aaaatcgcaa aaggcacgtg gttcaaaaga
    71281 taaggcagcg gagatgctga tggatcgatg tatttattat aagaactccc caccaggtgt
    71341 agaatgggac ggagtcttta caagaaaaca taaataattg ggtctataaa ttacatttat
    71401 ctttaggata cgagaaaatt ttttagatat acagagtgaa agagccaatg aggaacgcta
    71461 ttctacttga aattttttca tttcatggta aagtagctag tcaattatat agaaagctac
    71521 tttgtatcaa ttatccggca aaaagaaata ggataatctt gcctaaaaaa atgagaggaa
    71581 tttaatatta aattgttaaa tgtttatgaa gcaaagcgct gaatagaatg caatgcggag
    71641 tatcaaaatg aaaagaaaaa tattactatt tacattaatt acagtttgtg ttgatttttt
    71701 atcctgtaat ggcacagcag atggaaaatc gagtgtaggg caacttgttc ttttagcatt
    71761 tagagcagct cgattagcaa acgaaagagt tgaactatct ggaacggtaa aaggaccagg
    71821 ttctgtaaaa aatgcaagtg tgaaagtctt cccgacccca gcgaatggac aatgcatcaa
    71881 agaagatgga actttaaatg gaactcccat cgccactact aaatcagatg agaatgggaa
    71941 atacaatcta tcttacaaaa aaacgggaac tactgtttgt gtagttgttg ttccggatga
    72001 cggttctcag atcgatgtat tttcacctgc aagtagaaca aatgccccga agccatggtc
    72061 aggagcaaac aatttaatgg cagtagtcaa cgaaccttca ggcagagcaa atagcattgg
    72121 tgcgagtaaa actgtaaatg taacaccctt tacacgtatg gcagcaagaa gatttggagc
    72181 gttaagcact atagctcaaa acaatggagg gaaagtattt cttggaatag ttcctgtagg
    72241 acgcagagct cctggtgatg ataaaagagt agagttcgga aacaataatg caaccactat
    72301 aattgataaa gcaaatgaag atgtagagaa tgcctttttc ccaaagcaaa ataagacagg
    72361 agggttttcc atcgaatcaa cggatgtgaa tgatccgagt tatgctttaa aactaggaag
    72421 tatacttatc acagcagata agttaggtgg aggggttgtc aatggcagca ctaccgcttc
    72481 tgatttagaa aaggtaatca actttatgga agaggatttt tctgacggtc gtttcgatgg
    72541 gaaaaagata gatagtaata ctggcaaaat tgctcctcta acttcctcag acttaggggg
    72601 tgttgttgcg aatgcaaaag cagctgataa ttttctaaaa gttgatttta aggctgcaca
    72661 acaagagtat gattcctatg actcaagcgt tagttctaca tctgatgatt tatttttttg
    72721 cgaaacagat actttggatg cagcatgtaa tattgcagtt ttacctggat caccaccaga
    72781 aatttggatt tttaaccaag acgaggattt tattgatatt ggagatattt atgatcatgg
    72841 aagtgttggt tttggaaccg ggggaacggc tagcactcga tactatgcta ttgtcaatgg
    72901 aggaggatct gacctcaagt taacattgcc atttactttt tcgggaagtc aatttacagt
    72961 agtggaacaa ccagatgcgg tagttgccat gggtgatgca acttattttg ctgtaaaatt
    73021 tgttccatct tctgcgacta cttttagtaa cacaatgaca gtaaatagca atgataccgt
    73081 ttattctcct tatactgtga ccttgacagg agaaggagta gatttaggca ccaatttaca
    73141 gctctattgg aaattctcgg ggaatacaaa tgactcaagc ggtcaaaatc gcattggaac
    73201 taaagtggga agaactgttt tagcttcgct aacttatgac tattgggata atatagattc
    73261 tgcctttaat tttgatggaa caagtgatga gattacaaat acattttcgt tcgttcttgg
    73321 atcgactgga actttttctg catgggtatc acctgctgat ctaagcaatc cctcttattc
    73381 tattctaaag agaggaacta caccagaatt tcaattgatg ttccttgatg atgttacatt
    73441 aagatttaca gtcacgacga gtgttggggg aactttggat ttagatatag atattgaccc
    73501 agatgatttt attgatcaat ggaatttagt gacaggtgtt tatgatggat ctaatatgag
    73561 agtttatata aatggggagg aatatgattt ccaagcaaaa acaggaaccc ttacaaacag
    73621 cacaggattt tctgttggta atgatttttt tggaaatttt tttgatggag atattgatga
    73681 tgtaagagtt tacaatcggg cattaactcc tgcacaggtt tatgctttat acaaccaaga
    73741 ctaagtctta ttgacaatat tattactcaa tccttgaatc atctggattg agtctttgat
    73801 tcgactgagg ctatcctcgg agtagggaag cgtcatgcga atggtttgtt tgagttcgtg
    73861 aatttcttta tagatttcga tgagaatatt tacctgatca gttgcttcgt ttttatataa
    73921 atatttgagc attgcataga gacgagcagg tatgtccata tgacagactt ctgttggagt
    73981 tagatcttct atgtttgcaa agatccacat ttgcagattg agagaaatta ctttttcaat
    74041 tctagaaatg tcctctttcg tgtttttcga ttttagactt ttgtaaaatt tacaacgatt
    74101 gtctagatag tttagaatta cttttcggtc gatttctttt gatcgaatga gttcccattc
    74161 ctttcgtaga gactcgagtt gatttttacc gtatgcaact tgagctaatt cacagagatg
    74221 aactgccgtc ggttcaaaaa ttttcatacg aaatgtaagc gaatccaaat cgcctgcaac
    74281 taaactaaca gcagatccat tttctatata ctctaacgca tctgaaattt cttggatgaa
    74341 actggtgcgg agttgatctg taggatttgc taaaagataa aagttataat cagatagctc
    74401 attgtcgtca ccgcgtgatt tggatccgta aaagagtaac tgaaaaggat gaatcgattt
    74461 tactttatcc agttttactt ttacttcttc agagatagtt tcatcaatta atgtcatgtt
    74521 attcaaaaaa taatttcatt ctcgctagat tttcaatgat cgctttaaat gctctatctc
    74581 tttcgtcatt gcctttaaaa ggaagagact taatattatt taagtcatga taaataattt
    74641 cgatcgttgg attatttgca ttctttttaa ttgtagatac aaagtctaga ttaattacct
    74701 ctgtctcacc taattttaac cacattgttt tctcctattc aggttgataa cattaatata
    74761 tttcaaatta tcgtcaacta taagtttgaa atttattgac taaatttcat attgattcgg
    74821 tttattttgt ctgaaataac taaaggtata ttatgctttt atacaccaat gcatggcaac
    74881 atttccaaat acaaaatttt tatactctac attttgaaat ttattttctt ctaataattt
    74941 tttcaagttt tcttggtctg gatagtgaag agaggataca ggtaaatagt caaacatttc
    75001 atttttggat ccccagatta aatatccaag aagtggaacg attttaaaaa aataaaaatc
    75061 agcaaaaact cgaataatag gattttttac ttttcctaca tccaaattaa taaagagtcc
    75121 accaggtttt aaaatgcgat agatttcact cagagctttc tcaagattgt caacatttcg
    75181 cagaccgaag ccaatcgtta caacatcaaa acttgcattt gcaaaatttt ttaattgagt
    75241 agcatcgcca acttcttgtt tagcctttgg aatgccagtt aatctttgtt ttgcgatgtt
    75301 tagcatattc tcagaaaaat cgacagaaaa aagttccttt gtattttcta gttctgcaat
    75361 gcgtagagat atatcaccag tgccacaaca taaatccata caagtcaatt tttgataagg
    75421 aaaagtttgc tctatcttac ggatgataga gtttttccat tgacgatgca taaaaaaact
    75481 atttaagtca ttaaacaaat catacttaga agcgattacg tcaaaatttg attttacaaa
    75541 ttctgccttc acatttgctt ctggcatctt aaaattactc atagttttat cctaaattat
    75601 aaataataag atttggtctt gaaaaaaaac ttcagaattt tgaaaattgt atagagaggt
    75661 aacgcagatg gaatccctaa caacctattt tgttcctgta tttgccattt ttattttagt
    75721 ggaaaaccta attgcattta aaaaaaatcc agaacgcttt aaaataaagg attcttttac
    75781 tagcataatt atgggaatcg gaagtgtatt aatcaaccta gtaactgcag gaattgttta
    75841 caaagaatat tcttttgttt ctcagtatgc tttttttaaa attgatccaa catggtggtc
    75901 ttgggttgtt ttattatttg ctgaagattt ttgctattat tggtttcata gagtaggtca
    75961 tgaaagtaga tttttttggg cttcacatgt tgtgcaccat tcctcagaaa gttataatct
    76021 gtcgactgcc gttcgccaga cttggactgg aaccatgatc aatgcggtat tttggtttcc
    76081 tcttttgtta cttggatttc ctcctctcat ggttatgatg caacaatcca ttagtcttat
    76141 ttatcaattc ttcattcaca ccgaacaagt tcgaaaatta ggacctttag aattaattct
    76201 gaatactcct tctcatcata gagttcatca tggaagtgat ttaaaatatc tagatagaaa
    76261 ctatgcaggg atatttatca tttgggataa aatgtttggt agctttcaag gcgaagaaga
    76321 agttccaacc tatggtcttg tgaaaaatat tcatacttac aatccaatca aagttgccta
    76381 tattgaatgg tggaatatgt ttaaacaaat gtttcaagtc ggttcttttg gaaatgcaat
    76441 taaggtaatg tttaaacctc ctggttggag tccagaaggt ggaactacaa cggctgagtt
    76501 acaaaagcaa gcaggtctta ggagctaatt cacattagct cctaaataga aagtttactc
    76561 ctttaagaag gataattctg ttttttcatt gacgatactt ttatctttca tctctaccat
    76621 cagtgcatct tctgaggagg caagtttgat ttttatcttg ccatcgatgt caataattgg
    76681 ggagccttgc acataacttc ccttaaatgt ttttttattg tcaattttta cagtgaagtt
    76741 gtttccatct gtttgggtaa tctccataat cacattcttg gttcctacat ttccatccca
    76801 ctgcattgca gtccatgtcc ctgtaaattt ttcaaatttg gatgcaccac cgaagcttct
    76861 agttttagag gcaatttttg cagtggacag aactacagat gctacattgg aatagtcact
    76921 gtctgttcca agttgtgtct tagaagttac agaatagaga tagaattttt ctctttcagg
    76981 aatggtaaag gtataaaaca atttattcga atcaactctt gatttaaagt tccacttact
    77041 ttctccttgt tttttctccc agatattgta ttcttctgct ccatctactt tatgccaagt
    77101 gattgtagca aatccatttt tcttatcaac ggtagccgat acaccagaag gcggttctaa
    77161 tttagagcct cctcttctta aatttggatc tacataaccg actgcatatc tagaaaatgg
    77221 tccccaaata ttgtctttat tttttgcaat cacagtatag tatctttgtc cgcgtgttgc
    77281 gggagcgtct tctaattgat cttctgctgt ttcgcctaat gtctcccatt tattatccac
    77341 aaatttatag acaagatagc ccgttgctcc agatacttta ttccatttta caacaatctt
    77401 atctctgaat tgtcccatgg atgcaacaac tcgatctggc gcttgtggtt ttgcaatgag
    77461 tgaagttttg cccattacag catccgataa gattccagta attgaattat ttaaaccagc
    77521 tacagtatag gtttctaacg ttccattttt tttggcagtt ctatcttcat aaattgtgga
    77581 attggttttc gcaattgcgc gataagtctt ggtcgcgcta tcccatttaa aaatttgata
    77641 gccagttaca ttttccagag gctcccattc taagattatc ttatctgaaa attttccatc
    77701 ggtagccgtg atggatgtga tcttaggagg aatttcattc ttagtatctt tcgcataacc
    77761 gatggctgtt acttgactta aatctgaaac gtcagtttct gagactgcag taatcttata
    77821 cgaataagct atttctggca aaactccagt gtcttcgaac tgagtatttt gcgataaccc
    77881 tatttgttga aaattatcat cgtctggatt gcttcgataa atttcatacc caattgctcc
    77941 tgggacttgc atccatgtca ggacgatttt attcgaaaaa ttcccattcg atgcattgat
    78001 ttctgaagga ggatttatat ctgttggcgg taaaacggta ttctccggag gggtaggttt
    78061 aattttattg tcaacgattt gtggctctgg atttggatca attaaatcaa tcattacaaa
    78121 ggcagaccta caaacctttg caaaatattt gtaatcaata taaccaaatc ctttgtctcc
    78181 ccattcaaca ccccaagagt tttggaattt gaatgcgtta gtagagtcat catagccaac
    78241 tagtgtaatc gcatgacctc ctagattagc tcctttccct tcattgtaaa ttttcttacc
    78301 tatctcataa aaattttcat tgattaaaat ccctacaaca actggtcttc cttgagagag
    78361 gtgattcttg atttcacttg gttcattgta tcgaagtctt agaaactctt tcgctttgaa
    78421 ttttgcagct aacgatagat gttctgcagt aggttgtttc gaaaaattct tctcgttata
    78481 tggcattgcg tcccaaggta aaactccctt agacacaatt aatgccattg catccgaaat
    78541 cgatgatcca ttgtctctgc ctccattgat ttgattgtaa ataaaagcag gcgagaaaag
    78601 atttttatag tttggtgtat ttcctgtttt atagttccaa gtccctttgt cctttctttc
    78661 tataaattct tgatatgatt tatttgcgta agctgttgac catgcaacac aactactttg
    78721 ttgaccttgg tttccaacag ggggcattaa atcagataga tcgactttag agggaagacc
    78781 tctatgagcg attctatttg gatttgcttc ttttatagaa gaaagtaaat cataactatc
    78841 tggttttaaa ccagttccag aagtcttggg accaaatgtt tgtgattgga tactaattgt
    78901 aataataaaa cttattgtga ggaagaggat aatcttgatt agtctcatat aatctcctaa
    78961 tggattttta tttaccactt tctagtaatc ttttttctag ctcttctgtc cactctctga
    79021 atgcgaatac atgtggttca tagaggctaa tgattgtatg ccgatttcct tttccatctt
    79081 ttacttcttt gttgatgtaa aaaataacat attcaccttc tttgggtgga acttttaacc
    79141 atttaccata tacttctggt agaactgtaa aagctaaatc gattttttct ggcataggac
    79201 taactgcttt gtattgcttt aaaacttctg cagttgtatt gattgcaatt aagttcttgt
    79261 tgatttgctt ctcttttaga tttactagtt ttgtcttggc tatatagtca gattttttaa
    79321 acagttcttc gaaagaaggt ggggtaggtg ctgcttctgc atagagtaga atgcaaaaaa
    79381 aggaaagtaa gaatattttc atagtttctc catcgtatct tgcttggtat gacctttaaa
    79441 atcaaaatac ttttagaatt ctattttaga taacaagtga aagcaaatca ttttttcatt
    79501 gaaaaatagc caatatgacc taatttgtat cctattcata ctcataaata aaaaaatatc
    79561 actgatattg aatttcgaga ttttggtagt tatggaaaat atttctgatt accaatcaga
    79621 tttatgctat gaacgaatca taaatcaata tgcgttatgt aaaacgaaaa actcaccaag
    79681 ttggaaacaa ttccatagat atgtctagct taatggatat aatattcatt ttgcttattt
    79741 tcgttatgat gagtgtaagt tttgcaaaaa actttaagca attagaaatc gatatgccgg
    79801 atagtaaaac aggaaattca gagttgtccc ctgatattca aatctcaata aaagcaaatg
    79861 ataaatattt tctagattca aaagaaattt ccttttctga attatcctcg attggatcaa
    79921 aggggcaatt taacaataaa accgtcagct taaatgtaga gaaaaaagtt ccctacgaga
    79981 aattcattca aatgatagat attttaaaac ttggtaacac aaagaagata aatcttggaa
    80041 ctgaataatt atgttccttc tcactaaaga aatttattta ccatcattgg aaaaattgat
    80101 ttacaagaat ttgattttcg ggttcacttt gtaataaatt tttggcttca tcgaaggtag
    80161 gctttcatgg attccttttt agattttgca ttagaacgat tatcgatagc gggacaggtt
    80221 accgctaaac caatgtttgg tggtcatgga atttatctag aggacttgat aatcggaatg
    80281 gtcgtggaaa gcaccctcta tttaaaagta gacgactcta atatcaaaga ctacattcaa
    80341 gctggagtta aaccttttag ctatgaagcc aaaaatggga aaccaatctc catgtcgtat
    80401 tggtgtttac ctgctgacat tttagagtca aatgaagacc taccgaaatg gttgaacaaa
    80461 gcagttgaag cggcaagagc atccaaaaag aataaaacca aaaaacatgc aagagtgaaa
    80521 tcaaaagtag cagccattca aaagaagaag caagcaaaaa cgaaaacacc tgcttccaaa
    80581 aagcaatcta agaaaaaacc tgcaccagct aaaaagaaag caaagaaaaa agccaccgca
    80641 aaaaagaagg gtaaatgagc attagcctct caggctattt tcttttttct gatagattcc
    80701 tagcttgctc aattaatttc aaaaattctt ttctgtatcc ttctttgtca gaaccgatag
    80761 acttttgtgc tagatcatag gtatctttcc agtttgcttt tcctttgaat tcagaatccc
    80821 taagaagcat tccaaaagaa gcaacagatg cggcaaaacg atagtcggtt gaagtctgac
    80881 ctaaatctaa aattttattt tgaataggga cggtgagtag tgagctttta gcactatctg
    80941 gttctttgta tctaatttta atcgtcagta tttcatttaa gtcagctgat ttagttctct
    81001 ttctctcttc ttgatacttg agaggatcaa cagaaccagt attgctttca ccatcgattg
    81061 gtattaactc atacaaagca gtaacgttat gtcctgcccc aatatctcca gcatcttttt
    81121 tatcgtcatt aaagtcttca ttccttaaaa ctctattttc atacccaatc aatctatacg
    81181 attttacaaa tttcggattg aattcaagct ggagtttaac gtctttggca atagttacaa
    81241 gggtgcctcc agattgagta actaaaactt tttttgcctc ctgcaaactg tctatatagg
    81301 catagtttcc atttccctta tctgcaagat tttccattgt agagtctttt atatttccag
    81361 ttccaaaacc tagaactgtt agaaagactc ccgattctct ttctttttca atgagtcgaa
    81421 ttaaatctcc ttggcttgca attcctacat taaaatcccc atctgttgcg agtataacac
    81481 ggtttatccc ctgtttatta aaattctctc tcgctatttt atacgcaagt tgtattcctg
    81541 ctccaccatt ggtagatcca cccgcttcta atgcatctaa cgcattgatg attttatctt
    81601 tttgggaggc agaagtagca ggtaatacta gcccgcttgc tcctgcatag actacaattg
    81661 caattttatc tttttcggtc aattctccta gtagcatttt aagcccttgt tttaccaatg
    81721 gcagcttatc ggcagttagc atagaaccag aaacatccaa aaggaatacg agatttcggg
    81781 gaggcatttc ttttctttca atcgatttcc cttttattga aatcttcatt agatagttct
    81841 tctcattcca aggagcgtag gaaatttcat tcccaacgga aaatggttct ttgccgctag
    81901 gttcagagta gttgtattta aaataattga ttaactcttc gatacgaact gcatctttag
    81961 gaggtaaaga accgttgttt agaaatcttc tagtatttga ataagaggcg gtatcta
//
LOCUS       DAOQOS010000006        75321 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_108_length_75321_cov_6.369662, whole genome shotgun sequence.
ACCESSION   DAOQOS010000006 DAOQOS010000000
VERSION     DAOQOS010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 75321)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 75321)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..75321
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108_length_75321_cov_6.369662"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..508
                     /locus_tag="PLF66_02215"
     CDS             <1..508
                     /locus_tag="PLF66_02215"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HMZ62879.1"
                     /translation="RLATVDSLTQIPNRRYFDERLQAEWNRLAREIQPLSLILFDVDF
                     FKQFNDYYGHLAGDMCLIQIAKVASRSISRPADFVARYGGEEFVIVLPNTQEQGAIQI
                     AKKLIRNLQKESIIHEKSSVSKKVTVSIGIASLIPTHEKSCEELIQLADKALYDSKES
                     GRNKYSIS"
     gene            515..1882
                     /locus_tag="PLF66_02220"
     CDS             515..1882
                     /locus_tag="PLF66_02220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62880.1"
                     /translation="MGKIDSIQPPLHFIKPDFDINVYRLAKIGYPFKLYFSSDIRKIE
                     FLNIEILGDLYHQFIKKKIRLMIAFRHPNAYDPLCLVHTFFNLLPKYFQQSKIKIDDV
                     THFHFIWDRGIPMWAGDRIGKLYSKLGGTPIHRGKVDWKGLNSARNLFVNGRFPLAIA
                     PEGATNGLNEIIAPLEPGIGQLGLWCLDDLEKQARQEGVVILPLGIKYSYLKEDWRSL
                     EKLLDSFNQDIGGKEILAPEKNLDKMSPTQVRIYHKLYQAGINLLTLLEDYYRTHYGR
                     YTKCDDPANISMRLNTLMDHILSVPESFFMITPKGSIIDRCRKLEQASWNWIYREDIG
                     SDISLLQKKLYDRMAFLSKEMDWHMRLVESFVSVTGSYVQEKPSYERFTEITFLIFDT
                     ISKIKTGSGKERPDLGKYRVQLTAGSPLTINSFYELHKTGKRGTKEAIDQINTELYSR
                     MQSLL"
     gene            complement(1879..3579)
                     /locus_tag="PLF66_02225"
     CDS             complement(1879..3579)
                     /locus_tag="PLF66_02225"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017858469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HMZ62881.1"
                     /translation="MKKDVLVFNNPDEMLAEFIQKDMHIHISATIGRPNALISSLGRV
                     FKDTNPGFTISTVAFHSTAHVLSLKKLMKKAITGFLGDNYPKPAPNRLYMNVLNGQPF
                     EVELWSLLSLIQRLMAGAQHLPYYVTKSLVDSDLFWDKLNKSVFVVPDPNNPNENLTL
                     ISALNPDYTFVHGVCADENGNIVLPQPMGEGNWGALAAKQGVIATVEKFVPADKIPPE
                     YIHIPGVCVRAVCEAKFGAHPQSLRVPLALKSIPDFTDISSYRDDYNFIIEASTVGDS
                     DIAAQAFEMMWVDIKGGHETYLQLLGEERLESLKKPQKIRNKKLSQQPANESEQMIII
                     ASRAIVEHVVNKHYSTILAGIGAAHMAAWCAAYLLERKNIKVKVMAELGFYGVLPHYG
                     DIFLFSQLHANRSEQLSDIVQILGTLVPKDCLGVLGLAEIDRFGNINSTRLPNGKFMV
                     GSGGANDIASTSDCLVVGKAIKSRFVDNVSYTTSPGSNVFQVVCQFGRFTRNPEKSKY
                     ELTHYVPSPIDGELAPKDAVEKYTAWSAYSGEPIMEEPPSQDELDILRRLDPDKIFLG
                     "
     gene            complement(4214..5761)
                     /locus_tag="PLF66_02230"
     CDS             complement(4214..5761)
                     /locus_tag="PLF66_02230"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HMZ62882.1"
                     /translation="MDHKIELERNGEKAANYLRLFLIFIFSAGTLMGFLVQNFVSKIL
                     GNYVAGVCIYVIATVSSALILRAKKYTTNVKYYTMGLEIFGLGFVLFGFLRFEDPELL
                     TIAINDIVLYGIYFLLIAESILRFSPRFTLFTGISCTLLFTTLGFLIKAKGGDKAAFP
                     VTTLTIILGMMFIFAMTIASYFGTRFVRKVVMNFKDSEDKANIRKKELEELSEKNSVA
                     IQELNQIAQDINDILQESKLIASDQYQFARESLESTENFTKSIHSIASMAKLQDENCV
                     INSESISSLSDMTDQIKRWTLSIEEKGSKSLEFGKKGEKDLNSTLNEMQNISNSSLEA
                     SKILNIINSIANQTNLLALNAAIEAARAGEEGKGFEIVANEVSKLAESSARNSKQIAS
                     IIGAMKQAVTEGEKQMYFSSESLQGIILIIQQITKDVKDISIQITDQLNLILDNKKRT
                     ERIQQLAQEMKNVTEKEAKSSDHLKQSIQNIFQIANKLEEKINVLNYSGEKLKKVTNY
                     LKDGISN"
     gene            6446..8737
                     /locus_tag="PLF66_02235"
     CDS             6446..8737
                     /locus_tag="PLF66_02235"
                     /inference="COORDINATES: protein motif:HMM:NF012580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HMZ62883.1"
                     /translation="MNDRNLEPELDLSNCENEPIHIPNKIQPFGFILVVNPDTFQIVQ
                     CSKNVDRYLGLSPETILTKTLFDLIPTEQVDPIQDVIITKRVIYINPIDLIFLEEKIS
                     FRSTLHLTDGLLIIDNEPIDTSTFLDFYSSVNRLMDDLKRAKTILDMSNLIVTHIRQL
                     TRYDRVMVYEFDKDWNGTVIAESLSGDLYSFLGQHFPASDIPKQARDLYLRNPIRIIP
                     DVNYTPVQIIPEVNPINQKFIDLSKSSLRSVSPIHIEYLQNMGVKSTLVISVIINDTL
                     WGLIACHHYEPKYIDSKMRNMLEHFGNIFSYNVDILQNIRLKDIDIKFNSIQTELIKR
                     LNQEKDFFTGLSKCMPILLQVNSAAGVVLFFGDKSFKEGITPDRDFTHRLIDWLYLTH
                     SEAIVYSDCLSDIYTESESYIPIASGILAMRLSKFDKNYIIWFKPEMIQSVIWGGNPK
                     EKIIAIPNQDGFRLSPRKSFEKWEEVVKRHSTPWEKSEILFAEKLRNTFQELIANQIS
                     ILEAQNIELGIIIQEKVLEIKKTNRELQKVSEQIRQQNEKLKIQNENILTLYQELKNH
                     KNQLKAIFDNTKHIIFLLDTEGRLLFFNQVANEISFKLHGRPLTIGDTLKSFARSQEE
                     IDRIEKRFQKAMSGEGFVLEEEVFHEMFNKSAWFRIEYDPVYEEDSLIGVAILMTDVT
                     EIKMAAQKIENQNSALREIAFLQSHRVRRPLANILGLIHVFNQEDLSDPFNKTVLEKL
                     LFSTHELDEVVRSIVDRTYNFDE"
     gene            8730..9284
                     /locus_tag="PLF66_02240"
     CDS             8730..9284
                     /locus_tag="PLF66_02240"
                     /inference="COORDINATES: protein motif:HMM:NF013306.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biliverdin-producing heme oxygenase"
                     /protein_id="HMZ62884.1"
                     /translation="MNSIHLLLKENTDDLHTRLEQVGFVNKIMNQTLTLQDYKKLIRA
                     NYAFHDSVERNLRGIMELADLDIIWKMPFLEKDRFAVGDFIIDKIENPFEPENFFEAL
                     GSLYVLEGATLGGKTIRRQLQNLPVNMHYYGCYGEDGGFHWRRFLDILKRNVDTEEKA
                     NFALKGAKETFLFGIRCFEKICQL"
     gene            complement(9279..9812)
                     /locus_tag="PLF66_02245"
     CDS             complement(9279..9812)
                     /locus_tag="PLF66_02245"
                     /inference="COORDINATES: protein motif:HMM:NF014689.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HMZ62885.1"
                     /translation="MIASILIGISLSLDCFAISISQGIKNSKDRLKGILTLAFLFTLF
                     QCGMLVIGYFSGNFLSESFEAITKWISFLLLLWIGIRMVRESFEEEEEEDLPDNTKEY
                     LLLSITTSIDALAAGFALPQLQLDWKLTTIFVGLICLLFTLTGGFVGKKIGEKFGKKS
                     EIVGGVILIGLGIKILL"
     gene            10167..11018
                     /locus_tag="PLF66_02250"
     CDS             10167..11018
                     /locus_tag="PLF66_02250"
                     /inference="COORDINATES: protein motif:HMM:NF021501.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2225 domain-containing protein"
                     /protein_id="HMZ62886.1"
                     /translation="MSEHSKGLSNPVMTTIVTCPICFHDDVFFYSLKAKSLASRQNVF
                     SIPIYLDTPKYVYVDFNDYSFSVCPHCYFTSNRKQDFIYTDSIAGTKNKSTLLPKIIE
                     HWTENKKEVEDLLIDNFVDENSFKEPRTDEGIISSSKLGIYKTNLEILYKVPYSHLKR
                     ARAYLKLFYTIRKLYKNEDESILKKALEDLEYVFKESDFPEISYEYEVLYLISAIYMR
                     LGDEANTTQYFKVFDKTKSEVLQKAKANPQINTHEVTHWLNRAKNLWQDRSDNMVWEV
                     LNPWPRK"
     gene            11027..11902
                     /locus_tag="PLF66_02255"
     CDS             11027..11902
                     /locus_tag="PLF66_02255"
                     /inference="COORDINATES: protein motif:HMM:NF017206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NmrA family NAD(P)-binding protein"
                     /protein_id="HMZ62887.1"
                     /translation="MLIKKIGIIGATGMLGKPVVKALLAAGFEVYAMVRDIIKAKIEL
                     PIGVNLVVGDIGNPVEIENFLKQVEAIHLNLSIKQTELEKEFHTEEQGLKLLLEIAKR
                     RGIKRISYLSSLLMNYQGTNGFHWWVFTIKQNAVQQIKNSGIPYTIFYPSTFMENILH
                     RQINGDKLRLAGLSRYKMFFIAGDDYARQVANSYKILRAENREYPVQGLFPYTSDEAA
                     TLFAKFYKKEILKVQKMPMFLLSFLSRFSPKFDYLYKIIYSMNHYHERFQSELTWKEL
                     GKPILTIEEYAANHK"
     gene            complement(11893..12840)
                     /locus_tag="PLF66_02260"
     CDS             complement(11893..12840)
                     /locus_tag="PLF66_02260"
                     /inference="COORDINATES: protein motif:HMM:NF016086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HMZ62888.1"
                     /translation="MLSRIAESLFWLGRFIERAEGISISLQVQHSASLESDIYGFNNW
                     QPILNAVGQLSSFSKIHTEATSDKVIDYLVFSPENPNSILECIARARENARGVRDMIP
                     KESWEIINVTYHELNQFNMDLVRKEGPDKLFNFIRQRSYLFEGATEISMFRGKGYHFM
                     RAGKYLERADQIARILDVKYHIPLPRVEDVGNPLDIYQWKTLLDSTGAYEAYLQHYNI
                     KITPIQIVELLVFNPQLPRSLLFCLERVRQSFRNISSIPDKFYANKAEQKIGKLYYQI
                     AYSSAEEIFFFGLHEYLTNFINDLINAGNEINRTYFGYL"
     gene            complement(12842..14281)
                     /locus_tag="PLF66_02265"
     CDS             complement(12842..14281)
                     /locus_tag="PLF66_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002976013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HMZ62889.1"
                     /translation="MNIKLDYNETNPIYDEMFTTTDKPREHYKDVFEILATLGISEFK
                     QKEVLAKLVSINQGITFTVYSDGKGIERIFPFDLIPRIIRKMEWDTIEQGLQQRIKAL
                     NLFLTDIYSSGKIIKDKIIPGELVYTCPDYIQEMVGVHVPHNIYTHISGIDIIRDADG
                     EYYVLEDNLRTPSGISYVLENRIIMKRIFPEIFKESSVLRVDSYPALLHDMLMQLAPF
                     GKEKPTIVLLTPGVYNSAYYEHIFLASQMGIQLVESYDLIVSDYKVYMKTIEGLKQVD
                     VIYKRVDDSFIDPLVFRKDSVLGVPGLFESYKRGNVVLVNAIGNGVADDKAVYSYVPD
                     MIQYYLNEKPILKNIPTYRMGIEEEREEAFKKMDTLVVKATNQSGGYGMLIGRDASPE
                     EIEKYKKKIMENPRNYIAQPTINLSTAPCFFNGTIEPRHIDLRPFAIYSPNGIKLIPG
                     GLTRVALKKGSLVVNSSQGGGSKDTWVVS"
     gene            14540..15811
                     /locus_tag="PLF66_02270"
     CDS             14540..15811
                     /locus_tag="PLF66_02270"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HMZ62890.1"
                     /translation="MLIEEKLQQSPEDWTKWLAQGNVVPYFQPILSLENKSVLGYESL
                     ARIVFSDGTVKTIGDFFISEFNGSLDKQREFKKFQRLVDHELRKRALYLLTHDPNPNS
                     KLFLNISPAIMLSYLDEGNSENELQAFIRKVNELGISPDRIVLEITEDYIHKNLELLK
                     PLIAFYKDFGFLIAIDDLGSKSSNLDRIGIFQPNIIKVDMQMLRMSLVDRSYKEILYT
                     LSRLGESLGISLLFEGVETEDQMYQAMSFGSRYLQGFLFDKALPNVPDKEKYTKHLNH
                     LVDKFYAMKLDSIKQKVNWESRVEALIQKIADEIRFSEEGAILSYSEIFTVDPSIFRF
                     FVTDLQGNQLSPNYTKKKNSEIIIQRHARNCNWSWRPYFLNHLYESYRQGNPWVISQP
                     YNDIMENLLLRTFSKTFKDRFILFIDVLYEE"
     gene            complement(15879..16211)
                     /locus_tag="PLF66_02275"
     CDS             complement(15879..16211)
                     /locus_tag="PLF66_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010415576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62891.1"
                     /translation="MAPKPVKVTTNDLTTLEKNLKNKSDEAIGNYLFNGYRIQVSRYN
                     QSATERIRAFAKRRREQGLCVACGAKVKKVNPKTGQPYKYCDFHHDLALQKRKQLKTP
                     STKAKKKK"
     gene            17530..18408
                     /locus_tag="PLF66_02280"
     CDS             17530..18408
                     /locus_tag="PLF66_02280"
                     /inference="COORDINATES: protein motif:HMM:NF014543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62892.1"
                     /translation="MRSIIVILLFTIGFTSILANDGKCENGDCDNGRGVFIFSDGRKY
                     DGEFKAKEMNGLGILYFKNQNVYEGDFLKNKIEGKGRYTFANGDIYNGDVKDGKFHGR
                     GIFYTKDGSRYSGEFVENKFQGIGFFQYPEGQLYLGNFKDGRPFGLGIFKNSEDSRLA
                     GDFLDNKAISQGFAISLKKDKSFSNIENGQLLELRKLLYKNHKYYAGELKEGKLEGNG
                     YLISETGNEYFGNFKNGKLHGFGFLISSKGNYYIGNFKDGKFHGKGEFFNFAGELKVA
                     GEWENGKQIPSVNSNR"
     gene            18447..20354
                     /locus_tag="PLF66_02285"
     CDS             18447..20354
                     /locus_tag="PLF66_02285"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HMZ62893.1"
                     /translation="MFRVFILILFFFSFNSGFPQSQDFSFISYGNLPTWTGVGVDWSS
                     SPKVHIYDTKIYQGHAEFVSRGLGRKIRFNDFKKLARQSQNREFLPFFLYDLSSKPFI
                     NKSGSFNWAVRLENYNYDDKPAHLATEILKLVKMISELDKTFNGKGLIVLTEGDNNPL
                     GVSKLGKFILKEGHSFITLNELIKYVGGKKMEVLNPGVAFGLVKFIKNEKELEALLPT
                     DIAILNFIPLHIPPVSGIVSLKPQTPLSHVNLLAKNRGTFNGYVTDIFALQNLKSLIG
                     KYAKLSNNGDKIIITKSSKKEVDENQKLNPRKQIIIPEARQDLTEVISLTKENEKFIT
                     TSHIGAKAANYYLLLQNLPDLTRPAFAIGFKYYFDTIKGEPETMIQNFLKEKDKFSKE
                     EKSKALLKIRSAIEKSNSFPATLVTSIRKILEKYPPKTKIRLRSSTNNEDLPEFNGAG
                     LYESKGFNSKDNDEKLKSKILSVYSSLWSDRAFFERDYFGIDHSKVGMAILIQEAFQK
                     EVANGVIVTTGSDKKVQMIVNTQSGEHSVTSPEENILSESFLLNPESGSIEKIYSESS
                     LSPVFIGTKTNELQLKKIQEATMKLHSILIKQRIDAGDKNKYGIDIEFKIMHEKGKNK
                     LYFKQVRLLKY"
     gene            complement(20351..21121)
                     /locus_tag="PLF66_02290"
     CDS             complement(20351..21121)
                     /locus_tag="PLF66_02290"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF005500.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HMZ62894.1"
                     /translation="MKTKKEIVENWLPRYTGVNLEDFGQYILLTNFDNYVQKFAHWHD
                     VPIQGADRPMRSATAEGITIINFGMGSPNAATIMDCLSAIKPQACLFLGKCGGLKKKN
                     ELGDFILPIAAIRGEGTSNDYYPPEVPSLPAFALQKAISTTIRDYNQDYWTGTVYTTN
                     RRVWEWDEDFKEYLKKIRVMAIDMETATIFSTAFYNEIPAGALLLVSDQPMEPDGIKT
                     DESDKEVTNDYVDLHIRVGVDSLKQLINNGVTVKHLRY"
     gene            complement(21132..21527)
                     /locus_tag="PLF66_02295"
     CDS             complement(21132..21527)
                     /locus_tag="PLF66_02295"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004786505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HMZ62895.1"
                     /translation="MIITPIQIRFSDLDAMRRVNNSVYSSYLELGRMDFCKKYLEIQT
                     LEDIPFVLVRVELDILKSLRTGEDANVKTWVSRIGNTSWDFSAEIINPINNTIYARAK
                     TVQVYYDYRIDSKLTIPESFYKILQSEMQ"
     gene            complement(21524..22651)
                     /locus_tag="PLF66_02300"
     CDS             complement(21524..22651)
                     /locus_tag="PLF66_02300"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HMZ62896.1"
                     /translation="MKYLLNIIRRRLSQLRGIQIDKYILFNAMKNWNRFPRVAGVVFI
                     ASTSFLVQHILYPSVDRNSVFYPIYVITYSFLIITTVLFSFLVYFLTRNHLRIHFIYL
                     LYFHIFIIGLLFLNILNLYHGQDLSLYAFAVMSIPLILRTRIPHYSVLYITILVAFII
                     CLAIWHPTRTNGLMITQIFLYTFVGFMTTLTIEYARRKTFHLEEELRRKNTILETISV
                     KDPLTGVYNRRYMMDLLVHNILLSRKNSHPFCQGIVDIDHFKKINDTLGHLTGDDVLK
                     EIAKIIKSTVRSTDLVFRFGGEEFIILFPETNLESANIVMQRIRTVIESHSFIGVPWR
                     VTASGGFTLLSDKDSPDTLLQRSDELLYQAKKDGRNRIYSK"
     gene            complement(22787..23596)
                     /locus_tag="PLF66_02305"
     CDS             complement(22787..23596)
                     /locus_tag="PLF66_02305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62897.1"
                     /translation="MLKSILPTLYQELFPDSLLDFEITEKKATCDKCALAAGRSPDHR
                     SYEEHLKCCTFHPFQPNYSIGQVLLSTRTPKEIKERIQTKIKNREYVFPIGITAPLPH
                     QFDFAKRKKNEFGNREDWLCPYFDRKKKNCGIWSARNSTCISFHCLSSYGTQGFDFWG
                     ELSDYISYVEMALLEEALLQFKFTPIEISEQIRFLNPEKENRSFQKQEFLSEDFYKSI
                     WKNYFGKEEDFYKECYKKIKSFSKKDFKRILGKQGLEIEKKVMTAKKAVLL"
     gene            complement(23589..24800)
                     /locus_tag="PLF66_02310"
     CDS             complement(23589..24800)
                     /locus_tag="PLF66_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020771000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HMZ62898.1"
                     /translation="MKQIGIIGGGAAGYFGAIQIGNLLRNTAQVTLLEKGEEPLTKVK
                     ISGGGRCNVTHNLFDPVSLSEKYPRGKKELRAAFERFQPQDTIEWFFKRGVELKKEDD
                     GRMFPTTNQSSTIIDCFQKEANRAKVNIQLGSLVNGIYFEENKWKVITKEKEFYFDKL
                     LVATGSSRKVWEILEKLGHTIEKPVPSLFTFHITDERISELQGLSVPNAEVSIFPKGK
                     SQTGPILITHWGLSGPAILKLSAYEAKTFFDLDYKTKIKINWVYPNTNSEIQNKILET
                     KKEHPAKKISSFSLFHLPSRLWESFLKKLNLSDKRWSEISNAEINFLRDELANGIYEV
                     SGKSIFKEEFVTCGGVSRKEVDFRTMESKICKGLYFAGEVLDIDGVTGGFNFQNAWTT
                     SWIAAEAIANA"
     gene            24976..26073
                     /locus_tag="PLF66_02315"
     CDS             24976..26073
                     /locus_tag="PLF66_02315"
                     /inference="COORDINATES: protein motif:HMM:NF033749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohemerythrin"
                     /protein_id="HMZ62899.1"
                     /translation="MKLMYTESQDKMTSLRLFWEENHFQLGIPLIDIQHLWLLYIISN
                     LEAALSYSNTLNETMKDSIVGVIDYVSEHFSLEEEILKEFKYPQFKDHVAHHREFVET
                     LQTKHDLANSNELAALGLLNMLQKWLFNHILKEDKQYVEFFKSKSIDVRPFCQEILIQ
                     KKYNITKKQGDVYKEVCSFRQSEIEEVISENMIEDIMHIWRSHNLSLHIPVIDLQHIW
                     LLKMVIQLDKAVKTMGTTKKNEVFNQVVEEAIDYTVKHFTLEERIMKEFRYPEILNHF
                     HQHESFVDFIKQRRKENESGDTLAAFHLAQDLKTWLLSHIAHEDKKLAIYLKDKMKDV
                     NEFTRKLHHNGEIIIEPTQRNFYKKITKDTL"
     gene            complement(26117..26866)
                     /locus_tag="PLF66_02320"
     CDS             complement(26117..26866)
                     /locus_tag="PLF66_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004437022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HMZ62900.1"
                     /translation="MKIGIVKHLNARPLTYGFEKTGNHTLVFENPSVLKDLLLKKELD
                     LALISSVECIRNESVLSFSTSCGVCAKEKVRSILFFKNKKEIFPSEKIYTDSGSRSSV
                     ALLQTLLRKETNQLISVIPTDPKEILTQIENGFNSHLLFGDNALLAKWNSEFYEVYDL
                     AEWWYKSTGLSFIFAFWAFPKSSSYPDSIFLESLEYGISHIGEIIETEKRFPREMVEE
                     YLKKELHYKTTDLDISGFSLFKKLCTDWGIL"
     gene            27174..28919
                     /locus_tag="PLF66_02325"
     CDS             27174..28919
                     /locus_tag="PLF66_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016543658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HMZ62901.1"
                     /translation="MITGNFFLNNDDLLLHFHTITDWKELVDAYEHGFKDAAEFKKTG
                     NDKLAFAPSTTEEAVEYYKSTLESVGDLIGKQLAPKVQEMDKIGLKYDNGKVTFPKPM
                     VDAMNAVMEAGLLPYAIGRHYGGLGMPVTVQSMAMEMVSRADGAFGIALACFNLAETL
                     EKYGSKEQIEKYVPLMAEGKYCGAMALTEPDYGSDLPNLQTKATKDANGQWRINGAKR
                     FITHGCGFGDKQSIILTLARTGSPTSGARGLSFFIVKTQDVHIASIEKKMGLHCSPTC
                     EVVYENSPAELIGVEGHGLVKYSMGMMNTARLSIAAQSMGIASAAYYEAKKYASERVQ
                     FGKLIQEIPAVKKILTHMDREIVAMRCILLEASRTVDLYLWREEHLKKDGMDEKEIRK
                     DETIRFWEKLANLFTPLSKYYISETANTIADNALQVHGGSGYTEDYDVSKIYRDARIT
                     NIYEGTTQLQIVACIGGVVAGMTPTGFLRAYLDKETSKFSPSEKFLRVRKTFEKIVET
                     FTSIKESSVRDGLAFEVVESAARTINGMLIERSASRLDDTRSKERLLLAKEYHIDSIA
                     ILESNLIRIQERGVG"
     gene            29258..29875
                     /locus_tag="PLF66_02330"
     CDS             29258..29875
                     /locus_tag="PLF66_02330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62902.1"
                     /translation="MQDKIIVFQNKKIRRIWHEEEWYFSVVDVVSVLTESKDDLTARK
                     YWNKLAQRLRAEGSESVTNCHQLKLEANDGKKYATDCANTQTLFRIIQSEKDFAILTS
                     EISKATFGITPSEYKELKGLKKKNQNFRDHMTDLELIFTMLGEKVTTEISKTEKPNTF
                     GKNKKVAKRGGKVAGVARKQTEKELGRSVISKKNYLPKRKDIHHG"
     gene            30585..31424
                     /locus_tag="PLF66_02335"
     CDS             30585..31424
                     /locus_tag="PLF66_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008589497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HMZ62903.1"
                     /translation="MSQKQVSLKEGYVVSRELEEALLVAEVTGRPLLIKGEPGTGKTL
                     LAEHISKTRNLPIYKWHVKSTSLAKDGLYFYDAVSRLNDSRFSEGKDREKVHNIENYI
                     RMGPLGLAFESKQKCIVLIDEIDKADLEFPNDLLLELDQMEFVVVETNRIVKAKERPL
                     TIITSNNERELPDAFLRRCIFHYIEFPSRDLMEQIIRVHLPDIDSTLLQTAMKTFYKV
                     RRLEELKKKPSTSELLDWIQILLYQGAKLNPMDKIPFVGALIKNEEDLFQYSGQLHVP
                     QKE"
     gene            31426..32631
                     /locus_tag="PLF66_02340"
     CDS             31426..32631
                     /locus_tag="PLF66_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016548386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA containing CoxE family protein"
                     /protein_id="HMZ62904.1"
                     /translation="MFTQFFYQLKAKKIPVSTAELIDLLKAIDFFSNKNGSLSINEFY
                     SISRNCLIKDVKYYDDFDQVFAEVFKGQVTSNSEMKSLIEEWLKQAIDRVIPEEQKKQ
                     AKELNYEDVFKELQKRLQEQKERHDGGNKWVGTRGVSPFGNSGFNPNGVRAGGDSSGK
                     TAFDVIDERKFRDYRTDETLNVRQIKVALKKLRNLKKEGRLEFSIQKTIDKTTSNGGE
                     LELVYERSRKNDLKLLLLMDVGGSMTPHANRVSKLFSASHQLNHFKEFHYYYFHNIIY
                     DAVFTDASMSKKVSISKLTKKFRPDTKVIYVGDASMHPYELFNKTGMYDFYGYGYLKN
                     TKPEVKVKTGFDRMRDLTEYFKDSIWLNPDDERYWHHETVEAIWGLVPMFFLSIDGLD
                     RAIKKLLRK"
     gene            32718..33011
                     /locus_tag="PLF66_02345"
     CDS             32718..33011
                     /locus_tag="PLF66_02345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62905.1"
                     /translation="MKNILLIIIFFFSFQLIAFQTVSEAEFILVQKKSNKQERNKSEK
                     EDKDKKDEKDIGNKDSKDTKESKNPESLKEKIKEKLTKEKEKREGGRFGDKRF"
     gene            32992..34713
                     /locus_tag="PLF66_02350"
     CDS             32992..34713
                     /locus_tag="PLF66_02350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62906.1"
                     /translation="MATNVSKFKLIPICFILFFVGINSLIAQTKENPPSVKYAIEYID
                     ALDLSKGDYRMAIRGENPDEMIEILKGKSDHHFRVEIGDTKTQIYPGSVSQERRNLVE
                     FYLNVYDKVPAGKYKVKLYLEETTNSYRLVDQTDFEMPENSEEDKQPVVTGLSPMGGV
                     IGDTITMSGKNFGSDLDKIDIYFYDLDDHTIDMELPEDLEPSDYQPLEYMQMRAKISP
                     FYISSTLDGMQHVNFTIPTTPYLKELADKAFFRKSIKLKVFVNGRPSSFMKVTILPKN
                     WNKKVIALALVVTILGLGLISLMVGKLNFIPYVLLDKNTNTYSLSRFQAFTWTVVLTG
                     SYFYIAIAFGILLQNGRIPDFNPSLVGLMSISYAGFIASHFLNKKNPKNAISDTPPAL
                     SDLFMENGVIDITRLQLLLFTVVAVIVYLYNLYLSNTLNGLPDIPATLHGLLMSSQGG
                     YIGGKIFGEKIAVNRILPRKISSSDKKIDLHLIGAGFIEGMKVVIEGSHAEPVQVEFS
                     SPSTVSCSLPMEKEIGMRGIVLIPPSGSTIVIPNAIEIVNGSVTEAESETDLEVEKSN
                     GVKKKKG"
     gene            complement(34710..35858)
                     /locus_tag="PLF66_02355"
     CDS             complement(34710..35858)
                     /locus_tag="PLF66_02355"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HMZ62907.1"
                     /translation="MLNILFLSIIIFSFQTFANPLEKAIELEDMNQIKLLLKSTKYID
                     IKNAGGDTPLQHVIKTNQFDIAKLLIHSNANLEIKDKEGNTALLLAIQSALSEIVEDL
                     ILAGANIKIKNKEGLNAIEIAEQKLKEGEKYNYDTESISKIIEILESRQNINEKLFQT
                     ILDAKDEKRVRRNPKNILEYLLLLPSIPILEEEVDKKYSLRIKEFQKIRYDKNCKSVN
                     YIIDCIANGFPIFDTKNEYIKFKMEQDDITLTIFKQKNGRHLIGVDVYYGGDSNRHEN
                     HFFYYENQSWIEVTDKVLPELDIKLFLNKKDYPLIKNMEEINFHPRTYIELPQFGTNA
                     FFVLDENCYSDGLNEKKCKSFMKYFYRSEIPMKWDKNIGKFKIKANNR"
     gene            36430..37461
                     /locus_tag="PLF66_02360"
     CDS             36430..37461
                     /locus_tag="PLF66_02360"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM-DISM domain-containing protein"
                     /protein_id="HMZ62908.1"
                     /translation="MQGDKILYDRFSKNQTWEVHSKILKPQNNLLVIKIQGDPRIYMT
                     GLYNSNHYYFGFYKEILDVNRDITGIVLCFMYLMIGSYHLLLYFKRRKEVYNLFFGLD
                     SLGVFGYFIFRSSIPYDLGMDSFTALRLELTILFTLVPFFLFFIETLFFSKISKINLS
                     YAIVSLILILGVIFSKSYIVSNYILKIWQLSALISVIFIIYVFIKAIRKGNKDSKRLF
                     IGTLIFLLAAIIELVDSLFLKMSIGFTHYAFFAFNVGIATVLANRFLTLYQTIEGLNE
                     NLENKILVIEDLNQNLEKKVEDRTKELQQTLNEVRILKEKQDGDYFLTTLIVEPLISN
                     LVQNPKVKV"
     gene            37552..38655
                     /locus_tag="PLF66_02365"
     CDS             37552..38655
                     /locus_tag="PLF66_02365"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HMZ62909.1"
                     /translation="MDREYTVFINADAMGKSIQGAGGAIVLGVVFKSLISRVQMVKGN
                     QNIYPERWLKNSYIELQSIFESFEGSMLISAVFGLIDNETGCMYYINAEHPWISIYRD
                     EKTIFIENALSLHKIGTHLMSQSFSVKVYQLEDGDSIFIGSDGKDDVLLSYDSEGYRV
                     INEEESRFLQKVDDAKGDLELLYDKLVEGVELSDDFSVLKITYQNTNLTEYRRIDSSI
                     KTLLEKASAFEEKNDFTNAKKEYDNAYEIHSKNLAILKKRASLYFKMKDYSKANLLFQ
                     ECAELEPANSILLFNIQVTAKAAGNLDMAINFGERIHIRDPYWIENLYELSDSYYKSG
                     NIDRAIKILQFAEALSSKDPKVKRLYDRLHASN"
     gene            38727..40586
                     /locus_tag="PLF66_02370"
     CDS             38727..40586
                     /locus_tag="PLF66_02370"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HMZ62910.1"
                     /translation="MEWTQSIYLSVYSLGRFPVFLFSIIIAFYLLFHPARSIQSITAG
                     VYFLFASLFHFGFLFAHSVNHPFGAYGYYLTALSPFGIAILIQFAYIFPRDYLIKERK
                     IVLWITAIISLIAILDYSYFAYTSGVGAFTAGYGSYYISKLVPYSSVAFYLWSIFIFI
                     RKTIYHSRTFHAENKKSFSYLFSPGGKEAITSRNFALLNLLELINTSILTSGLLFRMF
                     PYIHFLTLMNISFFAINTFYVILFLRYLSGSTPISFKIINFGLLSSLILTIFAGNIQT
                     IYAEKNFHSIRLKEIETILLLKDLNELPSNDALENVEMILHLRENGSEIIYQKSDLNI
                     PDKLNLWEKTPGLIEYNQKPEYPNFSDLEKKEKEEFYLRLDSKNIQFYSVHYKGEWYG
                     IGFNFLQYRKWIHPQILSLFSLYFLCLVINFLLLPLNLAKNFSQPLEEILSGLYEKTE
                     MLLLPSRKNVKNELEILNETLLHIKEDIEQKEILQKEYNDLKEKLDETEDPKTKEGPL
                     KDNTIQKVKTVEEYLKENYNYELSREGLASMVNLSPGRLGKYFKLSTGLKISEYINQL
                     RVDRAKSLLIETNKTVIEIAFEVGFESLRTFNRVFFSEMQMNPGQFRQKNQTI"
     gene            40664..42391
                     /locus_tag="PLF66_02375"
     CDS             40664..42391
                     /locus_tag="PLF66_02375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HMZ62911.1"
                     /translation="MKAKNQSALKSFLILTVLSVLVNCGPELKNEKKNKTLNNLAILA
                     DAKPARSESNFNEANALNTRSLLGPDQIIPTSSLSQDSSSELLGNIYTNIKNGDSNVE
                     VNLPLEVTLLNGLIANNTGIKNSYFLTLDGSVLYSRVVVEGNKITLIPQIRLQPNRKY
                     IAIVGGIQNAKGESLGNLKINFKSRDLDYGLYWYGKFGVCEKYFPNLKNAFYNPASAT
                     VIFSHGLQTNSVSNDDPYGRKGFSYQMYYYLEDNFAGAKELNGLRKFTNHNWIDKGWN
                     TGIVFWNQFADDALHAAEAKIWNLVEGPNGSSYKTMDEAGTVSSNIWDRNLQFKGQTI
                     QVQSVGELLSLYVIDALKQNTSGNIRMTGHSLGNQMITNISGRVNASGIKIKRIALLD
                     PAWTDGAKSYLPKVTEKEMQVALNHNGAKALELGIVGRNFYVTEYARVILFTILNDQW
                     KNGIVIERYNSTILDLYLQPFMNPNQPLNQQIAVMDMKPWYYSATQLKEKHNSVRQHY
                     FWSMESSSPIECVIQFFNRIKTGDIALSAFTPDSKVREMMLGGKYWSQVEGRYTADPS
                     DDWYEVKKK"
     gene            42650..44239
                     /locus_tag="PLF66_02380"
     CDS             42650..44239
                     /locus_tag="PLF66_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001969726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HMZ62912.1"
                     /translation="MSKSTEEWGSRIGIILVVASGAIGLGNFLRFPGQAAKFGGGAFM
                     IPYIISFLILGIPVCLSEWIMGRMAGKHGHSAPNIFKNYLTGIPLQVTSAIALIIPIM
                     IYTYYVFIEAWCLAYAVDFLTGSINLHPSHTLGTSKTDTLIQTSTEHFNLLTGSLSNG
                     ASFQSSIVYYVLACYVMNFYLVYRGIAKGLEAFAKVAVPVLLICAFIILTKVLSLDNI
                     SAGLGRMWNPDWSALLRGEVWIAAAGQIFFSLSVGFGIVLTLASYLKDKDDVVLSGLS
                     AASLNEFVEVVFGGLITIPVGFLFLGASVVAFGTFGMGFVALPAIFSQMQGGQFFGAI
                     WFFILFIAALTSSVTMVQPGITFLEEGFHFTRRQSVPILFVFTLIITLPIVYFNKNLA
                     ALDHTDFWIGTFMIYILATIQILIYGWKIGTKEAREDGLKGALIGLPPGFDFIIKYIT
                     PTFLIIIFTAFVLSEDGLMNSFNKMSESYMLTKVSATFSAEDAINQARVARGVFVGII
                     SIFLFFVFLVNLSLRRKGELK"
     gene            complement(44358..44876)
                     /locus_tag="PLF66_02385"
     CDS             complement(44358..44876)
                     /locus_tag="PLF66_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HMZ62913.1"
                     /translation="MEIDDQTEELKPIRKNLFSIKKLSYARGERPQVECILCGVTEKN
                     PEVPNLVVHESTHSIVSVNLYPYNPGHIIIFPKRHTLVITDLSDEEVLDMHRMRVKAI
                     QTISKAWRAQGFNIGYNLGKNSGGSIPHIHEHIVPRFINEVGFLDVLDNTRIVIYDPY
                     EMLEEIRRLWKE"
     gene            44970..45830
                     /locus_tag="PLF66_02390"
     CDS             44970..45830
                     /locus_tag="PLF66_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017852667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HMZ62914.1"
                     /translation="MNLIYVVVLAMIIWGVSWASAKMITNYTSLEVILFWRLLVTFLS
                     LIPIMWITKDSFRIPKKGIPYAIAGGIVMLIYNALFFLGLREGLAGAGGVFVTTLNPI
                     INFFLVALFYKYRLHLLEYIGLSLGFIGGLIILKVWNLSLDQVLKGGNAYFILASFTW
                     AILTIITATSKSYVSPITYSLYLYGIGTIVSFFMALPKDWMGALAFDSNFWWNIFYLG
                     SISTSFGTTVYFIASSRLGSAKASSFIFLVPASAMLSSWGLLGETPQWTTIIGGLLAI
                     SAVYLLNRKK"
     gene            46131..46550
                     /locus_tag="PLF66_02395"
     CDS             46131..46550
                     /locus_tag="PLF66_02395"
                     /inference="COORDINATES: protein motif:HMM:NF013401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HMZ62915.1"
                     /translation="MDCKICDIHHTKSIPMLVGETFHWVIRHSEFTKNAPGYLYIEPK
                     RHVESYNDLIHGEFMQLGECMKMATEWIYRHFKPLKLYTITVSEAVPHLHFHLVPRFE
                     DSPKGIEFLQEALSGKLQATPKAQAELERILKLFEEN"
     gene            complement(46800..47126)
                     /locus_tag="PLF66_02400"
     CDS             complement(46800..47126)
                     /locus_tag="PLF66_02400"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HMZ62916.1"
                     /translation="MKYGLSKTDLELIVKYFNNKREIEKAVLFGSRAKGNFKQGSDID
                     IALFGEKITFDTITSINYYLNEESPMIYFFDILDYKSIESKELKDHIDRVGIEIYNKN
                     KELILT"
     gene            complement(47136..47531)
                     /locus_tag="PLF66_02405"
     CDS             complement(47136..47531)
                     /locus_tag="PLF66_02405"
                     /inference="COORDINATES: protein motif:HMM:NF020360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase substrate binding
                     protein"
                     /protein_id="HMZ62917.1"
                     /translation="MNQDIRWKQRYSNFKKAYDQLTEFMEVSELNKFEVQGLIQCFEY
                     TFELSWKTLKDYLEHEGYDVKTPREVIKKAYQVNIIEDGVIWLDALEKRNLMSHTYNE
                     QTTLQTKELITSKYYQMIKKLVEYFSKIE"
     gene            complement(47536..47772)
                     /locus_tag="PLF66_02410"
     CDS             complement(47536..47772)
                     /locus_tag="PLF66_02410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62918.1"
                     /translation="MGEFADLRNSSFEYLIDLLKSSLDNNDYSKEDIEKLLEMSKDEL
                     YSITLPAAYDEVLQNKWLDEAISIIHSNYLILQG"
     gene            complement(48013..50274)
                     /locus_tag="PLF66_02415"
     CDS             complement(48013..50274)
                     /locus_tag="PLF66_02415"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HMZ62919.1"
                     /translation="MGDNNNLDLPDTFNETVEKLRKKLITAVDTGNLADVKAILETGI
                     TPNFQLPDIASPINYAAARSSHDILELLLKHGSNPNFTTSGPVTTLGQACKTGRLDMV
                     NTLLSWGAVVDGYSAEHKKTSLMIAAEMGRKDIVDSLLAAKADPNFKDKTGFTAYDYA
                     QKNNHSSIVSLLAPLTRGVKLDDSLKVTESDLIGQEMAKTALKQVLAMVKVNEERKKN
                     SLPVFKVNLHAVFSGNSGTGKTTFARYYADEIKKIGILPKAHLVEVSRVDLVGEYIGQ
                     SAPRTSAIIEKARGGILFIDEAYSLKVDKNDTLGQESINTLIKYMEDYRDELVIILAG
                     YTDMMRSFLDLNPGLKSRIPNIVPFEDFSDEQIGILFDNLCSKHEMNISKEDRTFAIE
                     QILIKKRGKGFGNAREVRNVFERAVAQHCIRLNNLEPGKITKENLQKFIYSDITADPN
                     DEGGSEIALLPPVDPRQNPKSAIYKLHALRGMEEIKKEVQAMADYIRIRKLRKGASSS
                     RGLQLHLIFTGNPGTGKTTVARLIGDIYRELGVLPSGHLVEVDRSGLIGGYLGQTAIK
                     TKDCIEDARGGILFVDEAYSLFTEASSEDMYAREAVNTLLKYMEDYRDELVVIFAGYQ
                     EPMDKLMASSPGLLGRFSKTLVFPNFTDEELSGICYDICKNDGYELSEAAHNKLISQL
                     LERKEKDSEFANARTVRNILEQAFKNQAQRIISLSPRDLMNRKTLDTIEESDIGNFTL
                     SNFNQVKRVGFSF"
     gene            51466..52056
                     /locus_tag="PLF66_02420"
     CDS             51466..52056
                     /locus_tag="PLF66_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1349 domain-containing protein"
                     /protein_id="HMZ62920.1"
                     /translation="MFKFNTNNLQWFCKPKNFTVTEERIEIITEPNTDLWQKTYYGFQ
                     NDNAHIVYVTAKEKYFTFTVKTEFEYSELFDQCGIAIYQNSNNWVKAGIEFRNKNSSW
                     LGSVVTNNGYSDWATTELETNYKSMWYRISRRENDFCIENSVDGKIFKQLRIFHLFDA
                     TEDIRIGLLACSPKNNSFKAIFTNIEVTECLWEEHK"
     gene            52332..52961
                     /locus_tag="PLF66_02425"
     CDS             52332..52961
                     /locus_tag="PLF66_02425"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HMZ62921.1"
                     /translation="MIRKINPMSFFFGILFGFGLQIFTFSFIWAFAKIEINKINSPEV
                     IIPSVMAALLAAVLEEFIFRRFIYRSVLSRYGWKIAVSASALVFGILHTFNDHVSFFS
                     ILSIMVQAGILLPVLYTYTNSLPFVIGLHFAWNLIQMAIFGTPTSGTKSFGSLFEIKL
                     LGNELITGGNFGVEASIQASFICLVLFLGIINSKKFREHAKHTGKWSLK"
     gene            complement(53025..53453)
                     /locus_tag="PLF66_02430"
     CDS             complement(53025..53453)
                     /locus_tag="PLF66_02430"
                     /inference="COORDINATES: protein motif:HMM:NF020397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HMZ62922.1"
                     /translation="MAEIKTKSITKDVSEFINEIEDEAKRKDALTLLKIFKAVTRKKP
                     KLWSNGTIGFGEYHYKSERSSQEGDWMATGFSSRKTNLTIYIMSGVKKYPALLKKLGK
                     HKVSGGSCIYIKKTDDIDLSILKELIKVSISDLGKTYKIK"
     gene            54047..54754
                     /locus_tag="PLF66_02435"
     CDS             54047..54754
                     /locus_tag="PLF66_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002770558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HMZ62923.1"
                     /translation="MPIEKIQTQLGKLSNPTKAKFLQGYFKTGKGEYGEGDIFIGITV
                     PNLRKVAKEFEFISIEELTTLIRSKIHEERLLALLILGRKFHNKKADEKLKKEIYEFY
                     IKHLQYVNNWDLVDEASRTILGSYLLDRDRSILYKLVKSKNLWERRNSIIATFVFLQN
                     GESEDTIAISKELLEDKEDLMHKAVGWMLREMGKRVDVKLLEEFLNTYKSKMPRTMLR
                     YAIERLPEKKRKMYLSK"
     gene            complement(54915..55772)
                     /locus_tag="PLF66_02440"
     CDS             complement(54915..55772)
                     /locus_tag="PLF66_02440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62924.1"
                     /translation="MLKKEYIAFTSFLLVNTMLAMNCFTISVYDKMNQIRKRNYEEKT
                     GTKYINNKGIFSPTDFTIVPNVSAQWNQRDQIQINFSDETRKNFCIQTKPKEIYYENP
                     EIKECLEIETNDEQAFYKLKSNSLTQKLKKNFYSIEQIALNTNYPNDLVFILKNQSYL
                     IVHNSKEVDINTSIEVLKEKNEWISNFLDNQENTVSQKKINTMLEIKINTSNEQYIAF
                     LYKENPTHKILIIEWEYKEILKKSAVGKFILWTILLPFALAGDILLSPITIPLGLIFL
                     ILWNSRDRE"
     gene            complement(55953..57203)
                     /locus_tag="PLF66_02445"
     CDS             complement(55953..57203)
                     /locus_tag="PLF66_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002974708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HMZ62925.1"
                     /translation="MSTDSFIPPIHNYPFMWFMDAALKVLMKGVNNVDEIVITEEDKE
                     KLKTYKKERLIFISNHPSTKEPPISYIIGNHMYSRFNYMASREVFDWGNGLVGNMIQN
                     IGAFSVIAGASDRESLKTTRAILAKEGGKLVLFPEGEPTGAENDNLLPFQPGVSQLGF
                     WGYEDAIKQDPKADITVLPAFIKYRLSGSIEEIRNNVDKSLEVMEKRLGISKQGKDIV
                     HRLLSIGKRLVERAEKQFAIVPDENQTFDYRIGRLRHRILDTVAETVDLKKYNKEDHA
                     IDKLRKILSTFEMVFVGVPDPKGELPSLDKAKWGRKYCQRAYDFISIKTDYLTSFPSA
                     ERIYEWIYRFESELIGYTYQRPQKAFVHFAPPFKISEYYPEYKKDKKGTVENLTKRLR
                     DEIQKLLDREVERSVRLFPENYTF"
     gene            57338..58099
                     /gene="lmtA"
                     /locus_tag="PLF66_02450"
     CDS             57338..58099
                     /gene="lmtA"
                     /locus_tag="PLF66_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020774340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A Kdo2 1-phosphate O-methyltransferase"
                     /protein_id="HMZ62926.1"
                     /translation="MALIEEFEKQGSFLFRWRSYVPSVIIILSFFFIPDYRYPDGDYN
                     KHLIYVALCFLVSLFGLFIRCFTIGYAPEKTSGRNTKKQVAETVNQTGIYSLIRHPLY
                     VGNFFMFAGITLLTKSLSFIIIFFFIYWFYYERIMFTEEQFLRGKFSTQYLAWANKTP
                     ALVPKFTGYKTPDVSFSFKNILKREYPSLFGIMLMFVFYDVIIVYFNEPELVAKGFPD
                     LLKPIQIYTAIAGITFYVIVRIIVKFTKILHVEGR"
     gene            58361..58495
                     /locus_tag="PLF66_02455"
     CDS             58361..58495
                     /locus_tag="PLF66_02455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62927.1"
                     /translation="MTTLQLQKMNSIWKELPDEKFQLAINFILWLIQSDEVLADMEVK
                     "
     gene            58781..59197
                     /locus_tag="PLF66_02460"
     CDS             58781..59197
                     /locus_tag="PLF66_02460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62928.1"
                     /translation="MFINKIFLFLLTVIALALFGFIYLSHKIQDGMCANEIVSEKISL
                     DLKYKAIVFIRDCGATTNYSLQVSILPKQDSLENSTGNILILDESYIDFENHKTTLEY
                     DWKKDNSLSIQIDPKTKIYYQVDQYKDIRIQLNKIE"
     gene            59239..59964
                     /locus_tag="PLF66_02465"
     CDS             59239..59964
                     /locus_tag="PLF66_02465"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002771002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HMZ62929.1"
                     /translation="MKENIYSSEFVEGLFNRMSNSYERMNYFTSFGFSLRWRRQFLKF
                     LVPSVERVKIIDLMTGMGETWGAAKRRYPNSELTALDISDGMLNRARIKNQKKFEGSV
                     KIEKQDILKNKLPSNYFDIAICAFGLKTFTHSQIEKIAEETYRILKPGGQISFIEVSN
                     PSNRILSLLYSIHLGFVVPVIGFLFLGGPEEYKMLWRYTSAFGSSKEAFSSFQNQGFI
                     LEYKSYFFGCATGFTGKKPKSFS"
     gene            60257..60592
                     /locus_tag="PLF66_02470"
     CDS             60257..60592
                     /locus_tag="PLF66_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012390004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HMZ62930.1"
                     /translation="MEIKKRTSGDATILELTGSLDIYTATDFKTYLQANVNTKGAKVV
                     VDMEKLSYIDSSGIGMLIKSLNYMKELNGNLQLANLKESLQKIFKVAGLTAYFQFLNE
                     KEFKEKYST"
     gene            60589..61425
                     /locus_tag="PLF66_02475"
     CDS             60589..61425
                     /locus_tag="PLF66_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004754929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HMZ62931.1"
                     /translation="MNSLDLSDHVLEEVLSEIQIQSLDCTWNITNEKLIDVMGIRKED
                     FYRYLYNLRRNHTQSISLRSFSEKEADLLCILLEQILKRNDIEEQFIRSGIYFPETAL
                     EELGQILVELIQSSLKKHKLDKELFHLLVSSTKLFDDAFDSYFADKFDMEVLFERSIS
                     IFINSKNIDNSFGAEIFLKKHLQSLLKYNKISLAKITKEYRQRFYYELFGKFKREGKM
                     DSETKRLLEFFELEDGATLKDLKKRFKELLLIYHPDVNKEGHAKTQEIIENYNKLFEL
                     IR"
     gene            complement(61426..62214)
                     /locus_tag="PLF66_02480"
     CDS             complement(61426..62214)
                     /locus_tag="PLF66_02480"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HMZ62932.1"
                     /translation="MNQVYDCIASVVVYDTSLDVLLECVNSFLDTKLRVKIYIIDNSA
                     VKGNYPLPDDPRIYYHFNHGRNVGYGQGNNIAIENAEESKYFLILNPDVFIKEDALEK
                     LIHFMDMNPEIGISIPKVLNLDGSQQFVNKRHPAILPLFIRRFIPKILHPLFKKTLDF
                     YEMKDLNQEENYEVPFMSGAFMLFRRNVLDKVRGFDPRYFLYFEDADISREVQALGYK
                     TTYFARANVTHLWKRAAHKNWKITFIFISNMIRYFNKWGWSFLE"
     gene            complement(62198..63373)
                     /locus_tag="PLF66_02485"
     CDS             complement(62198..63373)
                     /locus_tag="PLF66_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008594868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HMZ62933.1"
                     /translation="MTTKNDLFTPSDAHAELRENVSRFAKQELDDQAKENDEKETFNK
                     DVFQKLAKELGLLGVTVPEKDGGLGLDSVAAVIIHEEMSKYDPGFTLSYLAHEVLFVN
                     NFYYSGNESQKKRYLQKVMSGEWIAGMGMTEPGAGTDVLGMTTIATKSNGKYILNGTK
                     QYITNGSIGQIFLVYAKMHKRDIKKTTAFLVESSFKGFSVGKKEEKMGMRSSPTSQLV
                     FEEMEVPEENLIGEENGAISHMMRNLEIERLTLAAQSLGIAKRCLDIMSEYSILHREA
                     FGKKLSEFGQIQRMIAESYAEYSAARALVYQVAANIHPESRNSLGAASAKLFTTQMAE
                     RVSRNAIQVLGGYGYCREYPVERLHRDSILLSIGGGTNEAMQKNIISDLKKQYESGV"
     gene            complement(63370..63885)
                     /gene="hpt"
                     /locus_tag="PLF66_02490"
     CDS             complement(63370..63885)
                     /gene="hpt"
                     /locus_tag="PLF66_02490"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HMZ62934.1"
                     /translation="MKKLISREKLKKRIKELGKEISNYYKKEEVVLLCILKGGFIFSA
                     DLMREISSPTTIDFLEASSYGDGKESSGTVLIKRDVGIDIKGKRLLIVEDIIDTGISL
                     NFLLKYLKKKKPASIEICSLLVKYKKHKVKSNIRFVGFEIEDDFVVGYGMDYRGKYRN
                     LDYIGIYEDLP"
     gene            complement(63882..64403)
                     /locus_tag="PLF66_02495"
     CDS             complement(63882..64403)
                     /locus_tag="PLF66_02495"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HMZ62935.1"
                     /translation="MDSLKTIYLLRHAKSDWEAEFRFDHERPISERGRKNAKALRNFL
                     QDRNFQVDKAYVSDAKRTIETLEIINKDKDLFKEIEITHLLYEANFGIFFNLITTTED
                     SCNSILFLGHNPDLEELANQLMLRQNAYKEFSFFQKFGTSSIIGLAFPISSWKNISEY
                     PGKLTLFWTPAKK"
     gene            64546..65832
                     /locus_tag="PLF66_02500"
     CDS             64546..65832
                     /locus_tag="PLF66_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011669513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HMZ62936.1"
                     /translation="MSSYNNAELNLDGKKFPLNLITGTDGKQGIDIKDLYKNTGVITV
                     DPGCFNTAIGESSVSRRDPDKGELSYRGYQVGDLAKNSTFVETSYLLIYGELPNKNQL
                     QDYSNRLSKHSMIHEDMINLFDGFPGKAHPLAVLSTMVMALSSYYTDEYEESADRGVD
                     YVTRLLSKIRTIAAFSYKRMIGQPFIYPIDKLPFCTNFLHMLFSVPSESYEVPKDQDK
                     LLNQLWILYGDHEQNVAATTVQLVGSTKANLFASISAGISALWGSREGGQSVAAVELL
                     ENILNSGKDYKTYLSDSNVRAELSRSNFLGHEAYNVKSPRAIVAREIFREYFKTHHSP
                     LVDLAFEVDEYISNEAYFQDKRLYPNLEFYSGILFHSIGIPKNMFTLMQAIGKLPGWL
                     AHWRELRIRSNFKKARPRQIYIGNLNKTYIPVDDRK"
     gene            complement(66008..66988)
                     /locus_tag="PLF66_02505"
     CDS             complement(66008..66988)
                     /locus_tag="PLF66_02505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62937.1"
                     /translation="MKNKLYLFLFCSIGLQFGCMQYCLRDTSSFELKKQELKFLKSKK
                     FALVGFFPYHFANGMRGIQKEEPHFPCSYYDTLKDIDKSQATYNCIVEFINDGKKDAY
                     PDLYKKIFVSVYGGKYQPSYYAPATIPYDTSTEAYFPTENEVRKQKVSIGDNSIPDEN
                     LRKFLLNHLSLFRHAGLPELESILSFEYAKIKDTCSCNLENKQEKLVKIQLKKMEVDY
                     WILANHKQLYSGYGDRSNYENEPTLKVLTFLPAILTYGTIPYWDEAVFESKFFIYDKN
                     LNLVKTFTFTNKYDFIAAWWLMLKKQNYASAYEPDVKELTKELHKMIQTQ"
     gene            complement(67053..68483)
                     /locus_tag="PLF66_02510"
     CDS             complement(67053..68483)
                     /locus_tag="PLF66_02510"
                     /inference="COORDINATES: protein motif:HMM:NF012912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HMZ62938.1"
                     /translation="MQKQKYKNFILVGLGFGDEGKGSITDFLVDAYFTKTVIRYNGGS
                     QAAHTVVTPDKKTHIFSQFGSGTFHKNTKTYLSEQMFIDLYSLLEEEFVLKQKGIKDA
                     LKRIVISPNATLITPYHKMIGQMEEIARGKNKVGTTGKGVGIAAIDKMYFPKEAIYIK
                     DLDSKNLLIKLEKQREKFFNRAISILSNTENKEVEKILLYFKNKYPLRQVFQFFQEFA
                     KSYQNTIDKGNSFKKRLLAGNESYLLEGAQGTLLDLEYGFFPYITKTKTTLESAKNLF
                     LSKKIESIGILRCYSHRHGKGPLPTETKILSKKIKEEHNLENHWQGSFRMGWFDLILT
                     RYAVKLNPDLSSIALTCLDQVSDFPKIKVCVEYIYNGKDSNLLRKFFKTKSENNEIWI
                     LDIIPQRHSLEDRKLLTKLLLECKPSRYLEFTTWKKDLDSLRENKIPKELQFFLDFLE
                     SKNGIHLPISILSFGKTRKEKMILKT"
     gene            complement(68464..69363)
                     /locus_tag="PLF66_02515"
     CDS             complement(68464..69363)
                     /locus_tag="PLF66_02515"
                     /inference="COORDINATES: protein motif:HMM:NF012449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HMZ62939.1"
                     /translation="MKIFATLPPPHREKSLQLVIDHPLVDSLRFNTGVRVSTNPKETL
                     ETLLRFCNGKDFWLDLKGRQLRISQWAVPTFGDIVLNHEIDVSLPAEIYFRNEEKSTI
                     RKIKGNKIYVDPPPPKALGAGQAVNIPDESLKIQGYFTEEDLEYLDAAKELGIVNIML
                     SFVEEESDIVQLTKIIPNANIICKIESKKGIQFVKEVFPNLKNKIRLMAARDDLFIQI
                     GENKSHIFPTLKSIIEADETAILASRLFTSLETSLEVSLSDLSDFILMKTLGYSYFLL
                     SDGICGNEKAFLKAMEAFAKTKI"
     gene            complement(69360..70316)
                     /locus_tag="PLF66_02520"
     CDS             complement(69360..70316)
                     /locus_tag="PLF66_02520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62940.1"
                     /translation="MGGGSYDRDVGSTSSSSDYDFSDYTDTAKKALKNTSASKQVLPL
                     KRNLVCKNKSPIVVAIDGTGSMGNDAYIIYDKMPMFYGQILMQGYLEDPSISFAVVGD
                     AYSDMSPIQVCDFEQGTKLDEWLEKLWIEHNGGGQSRESYDLTAYFYANHCKLEKADL
                     PFFFFIGDEGFYPKTEAEHITKHIGDTEKDVEAKSSFRKLNEMFNVFLIHKRYGTDDD
                     KIVEQWANVVNRERILVLEDSKAIVDVMLGAIAIVSESRSLDDYLTDMQDRDQTTDRI
                     KLVEKTLSGLSESTAIAKVDLKKKLPAKKPTTKKRQSKTKRT"
     gene            complement(70571..71665)
                     /locus_tag="PLF66_02525"
     CDS             complement(70571..71665)
                     /locus_tag="PLF66_02525"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HMZ62941.1"
                     /translation="MDTKELKEFHSKIIQFSSPEELFGEIKDDLPQNKLISLNEIFKD
                     LIKTFHPDKFNKKDAFTKYLAGEISSIINGLYAEAKKRIEKDIYGKTNLSVNTTASFE
                     IKTKLRTYKIFEQFLEADYADLYMGEFINKNSNPEKICVKIMKDKADNDLLRNEIFIL
                     RSIQHKSIPVFIDQFRTTSGELGLIERFIEGSDLITIRDKFTDGIPVEHMCWILDRLL
                     SVLGYLHYNKIIHGNIEPGNIIVRPSDHNVFLIDYLFSIPNANTTSNTYSCFNESYSA
                     PEVALKKKPIPSSDLFSLGKSMLYLMGGDVETDAFPSNVDKRIQDFLLEFLNPNPIKR
                     VRDAWQLWHRLSNLREEVFGARHQFLELIL"
     gene            complement(71674..72147)
                     /locus_tag="PLF66_02530"
     CDS             complement(71674..72147)
                     /locus_tag="PLF66_02530"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016761092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HMZ62942.1"
                     /translation="MKGYRKNVGVVVFNAKGFVLLGNRISHRSSWQFPQGGLDEGEEP
                     IVAAKRELYEEVGIDNAEFIYEVPEWLSYDFPANLDLPHMKDYKGQTQKWFLAYWNHP
                     ASECKLDIHEREFETVQFFPLKNAANTIVDFKKEIYKKLITILGPEIEKYIKQIK"
     gene            complement(72144..74273)
                     /locus_tag="PLF66_02535"
     CDS             complement(72144..74273)
                     /locus_tag="PLF66_02535"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HMZ62943.1"
                     /translation="MKLILLLILFSISIYSQNFEIQTIDLKKEFTDLNSPKSKIHTWW
                     ATSQEINPKANSNDPLSKTNSFDLPDKIWVSTHIPGNPVAENIVNENSKYFWYRKVFI
                     LKEIPEEDQVLYIQRISDSDETYLNGTLIGRTGDWESDKAQAYDKARIYSINKELFRK
                     DAPNILLIHVKKYFKESAGIWGEQNLIGKNKPILREYYLSNIKPLIFLFCYFSVGSYF
                     IFLFLRRRKERENLYFGLFTLGFVAYHALRNQLRFEFGFTYFEMKKVEFQILILLLVF
                     LYLFFRYYFISTENQFLRRLHYLNSIPLLSLVGLFIYMIFTTDIDQWNSLLNAYVIPF
                     LWTPLLISIFLIITYNAFFKGNNDAKIMFAGWSMLISSIVFDILIHLGFFQMERFTEY
                     GFFVFILSLAFILSNRFVRVHNEVEDLNQNLEKKVILRTEELQKALTRVNELKLSQDG
                     DYFLTYLLLEPLTVKAISSPNINIDFLIRQKKKFSFHNENMEIGGDTCIAHTISLQNK
                     SMTLFLNADAMGKSMQGAGGILVLNSIFESIVKRTNMNPFFANQTPENWLINAFNELH
                     NVFIMFDGSMMISLNMGIIEDKTGRLCYINAEHPAIVLYRDEKANFLPLDETITKVGS
                     PLLEETVSLNYYQLQDHDVLIIGSDGRDDLLLPNGDINDNFDIFSTHVEKGKGNLELI
                     LKEIEKIGEIYDDLSLMRIEYRKGETS"
     gene            complement(74283..>75321)
                     /locus_tag="PLF66_02540"
     CDS             complement(74283..>75321)
                     /locus_tag="PLF66_02540"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HMZ62944.1"
                     /translation="YLIEKGSNLESSDANGNTILITSVLSENLKLLQFLIKKKVDLNI
                     LNAQGKSPLYLASEFGKTEAAKILLNYPVEIDLIVEESYNKNYTPLLIASKNGNEKIV
                     SLLLEKKANINYKTKNNQETALILAAANGHLSVVKILLENGADINIKSNGYPYGTALE
                     IAQRKGYNQIADLINLHKKSGSTPRNSTLHKVSLENLIQLNNLISEEIKGRGKDGFTA
                     LHWACSKGNKEIVQLLLNSENINAKTKEGLTPLHYAIKTENLEIVKLLVAAGADKESK
                     DKKGNTPLATARKTIQDSILKIYSTTPVTSQKKETNSNIVLPPSTKNSSRNTKIDSDD
                     EDIEDDDLEEE"
BASE COUNT        24522 a        11986 c        13013 g        25800 t
ORIGIN      
        1 acgattagcc actgttgact ctttgacaca aattccaaat agacgttatt ttgatgaaag
       61 actacaggca gagtggaatc gacttgcaag agaaatccaa cctctttctt taattttatt
      121 tgatgttgat ttttttaaac aattcaatga ttattatggt catttagctg gggatatgtg
      181 cttgatccaa atagcaaaag tagcatctag gtcaattagt cgaccggctg attttgttgc
      241 acgttatggt ggtgaggaat ttgtaattgt tttgccaaac actcaggaac agggagcgat
      301 tcagattgca aaaaaattaa tacgcaatct tcagaaagaa tcaattattc atgaaaaatc
      361 aagcgttagt aaaaaggtta ccgttagtat tggtattgct agtttgattc caacacatga
      421 aaagtcctgt gaagagttga ttcaactcgc tgataaggct ctttatgatt ccaaagaatc
      481 tggacgtaat aaatattcta taagttaaat tcaaatggga aaaattgatt ctattcaacc
      541 accacttcat tttataaaac cagactttga tataaatgtt taccgtttag ctaagatagg
      601 gtatccattt aaattgtatt tttcatccga tatacgaaag attgagtttt taaatattga
      661 aattcttgga gatctttatc atcaatttat aaagaaaaaa attagactca tgattgcatt
      721 tagacatcca aatgcatatg atcctctttg tttagttcat acttttttca atctgctacc
      781 taaatatttt caacaatcta aaatcaaaat agatgatgtt acacattttc attttatttg
      841 ggatagagga attcctatgt gggcaggcga tcgaatcgga aaactttatt ctaaattagg
      901 aggaactcca atccatcgag ggaaggttga ttggaaagga ttaaattccg ctcgaaatct
      961 atttgtaaat ggaagatttc ctcttgcaat tgcaccagag ggagcgacta acggtttaaa
     1021 tgaaattatt gctccactag aacctgggat tggacagttg gggctatggt gtttggatga
     1081 cctagaaaag caagcaaggc aagaaggggt tgttattttg ccccttggaa ttaaatattc
     1141 ttatttaaaa gaagattgga gatcattaga aaaattatta gattcattta accaagatat
     1201 tggaggcaaa gagatattag ccccagaaaa aaatcttgat aaaatgagtc cgacacaagt
     1261 gagaatttat cataagctat atcaagcagg aataaattta cttacattac tggaagatta
     1321 ttatagaact cattacggac gatacactaa atgtgatgat ccagccaata tttcgatgcg
     1381 attaaataca ctgatggatc atattctctc cgttcctgaa agctttttta tgattacacc
     1441 aaaaggatct atcattgatc gatgcagaaa attagaacaa gctagttgga attggatata
     1501 tagggaagat attggatctg atatttctct gttacagaag aaattatatg atcgcatggc
     1561 gtttctgtct aaagaaatgg attggcatat gcgacttgtt gagagctttg tatctgtcac
     1621 tggaagttac gtccaagaaa aaccaagcta tgaaagattt acagaaatta cttttctaat
     1681 ctttgatact atttcaaaaa tcaaaaccgg ttctggaaaa gaaagaccag acttaggaaa
     1741 ataccgtgtt caactaactg cagggagtcc acttactatt aattcttttt atgaactaca
     1801 taaaactggt aagcgaggaa ctaaagaagc aatcgatcaa atcaacacag aactctattc
     1861 taggatgcaa tcgcttcttt aacctaggaa aatcttatct ggatctagtc ttcttaaaat
     1921 atccagttcg tcttgagatg gcggttcttc cataatcggc tctccagaat aagcagacca
     1981 agcagtgtat ttttctacag cgtctttagg agcaagttct ccatcgattg gtgatggaac
     2041 gtaatgagtc agttcatatt ttgatttttc tgggttacgg gtgaagcgac caaattggca
     2101 gactacttga aaaacgttag atcctggcga agtagtataa ctaacgttat ccacaaagcg
     2161 gcttttaata gccttcccga ctacaagaca atctgaggta gaagctatat catttgctcc
     2221 gcctgatccc accatgaatt ttccgtttgg aagtctggta gagttgatat ttccaaaacg
     2281 atcaatttca gcaagaccca atacaccgag acaatctttt ggaaccagag ttcctaagat
     2341 ttgcacaatg tcggaaagtt gttcggatcg atttgcatgc aattggctaa acaagaaaat
     2401 atctccataa tgaggaagga caccataaaa tcctaattct gccattacct ttactttgat
     2461 attttttctt tcgagtaaat acgcagcaca ccaagcagcc atatgtgctg ctccaattcc
     2521 tgcaaggatg gtagagtagt gtttgtttac tacatgttct acaattgctc tagatgcaat
     2581 gatgatcatt tgttctgatt catttgctgg ttgttgggag agttttttat ttcgtatctt
     2641 ttgaggtttc ttgagacttt cgagtctttc ttcccctaga agctgtaaat aggtttcatg
     2701 tccacctttg atatccaccc acatcatttc aaatgcttga gcagcaatat cagagtctcc
     2761 aacggtagat gcttctataa taaagttata atcatctcta taagaggaaa tatcagtgaa
     2821 gtctggaatg ctttttaatg caagagggac tcgaagagat tgtggatgtg ctccaaattt
     2881 tgcttcgcag acagctcgca cgcatactcc tggaatatga atgtattcag gcggaatttt
     2941 gtctgcagga acaaattttt ccacagttgc gatgactccc tgctttgcgg ctaatgctcc
     3001 ccagtttcct tctcccatcg gttgtggaag tacgatattt ccattctcgt cagcacagac
     3061 cccatgaaca aatgtatagt ctgggtttaa tgcactgatc agtgttaaat tctcatttgg
     3121 attgtttgga tctggaacta caaagacgga tttatttaat ttatcccaaa ataaatcact
     3181 gtccacaagg gatttggtta cataataggg aagatgctga gcacctgcca tcaatctttg
     3241 aatcaaactg agcagggacc aaagttccac ttcaaatggt tgtccattta agacattcat
     3301 atagagtcgg tttggtgccg gtttaggata attatcacct aagaagccag tgatcgcttt
     3361 tttcattaat ttcttaagcg acaaaacgtg agcagttgag tggaacgcaa cagtgctgat
     3421 tgtaaaacct ggatttgtat ctttaaatac tcttccgagc gaactaatca atgcatttgg
     3481 tctgccaatt gtcgctgaaa tatgaatatg catatccttt tggataaact cagctagcat
     3541 ctcatcggga ttgttgaaga ccagaacatc ttttttcata tgaattcaaa aataaacttt
     3601 tcaatgggcg tttgaaatga gcaagcattt aaaaacattt tgagaatagg aatgtaaaaa
     3661 aatttctgaa agaatgataa acgatgaatc agtttgtgtt taataagaag ctgaatttaa
     3721 tttgaattga taaaaaatac aaatctactc caattcctta tttgtatttt tggaacagga
     3781 ttgtatagaa tctattccag tttaattttt ttgatttttg gagtttttgg tttttaattt
     3841 ttatgaatcc tttgaaacta tagaaaaata gaataactca ctgaattatt tgataaaaat
     3901 ttagaatcaa ctggatagat aattttactg aaaattctat ttggatttgt ctttatcaaa
     3961 attatttaat ttggtatctc ctttcggaaa acgtttaata tctattggag tcgagtcgat
     4021 ctttctaaaa ttatccattg tttgatttac gatttagaaa atagagatag gttagggaga
     4081 aattggagta ttagtgtttg tagtcctaaa aatcaccaat aaaatttctt ctttttatga
     4141 ttcattgcga ccaacgttgc ttacatggct tgaaacgatt cttgcctaac ccaagttagt
     4201 ttatcttgag agcttagttt gaaattccat cctttaaata attggtgact ttctttaact
     4261 tttctccact ataatttaag acatttattt tttcttctag tttgtttgct atttgaaata
     4321 tattctgaat actttgtttt aaatgatctg aactctttgc ttctttttca gttacattct
     4381 tcatttcttg tgctagttgt tgtattcttt ccgttctttt tttattgtct aatatcagat
     4441 ttaattgatc tgtgatttga atcgatatat cctttacgtc ttttgtgatt tgttggatta
     4501 ttagtatgat tccctgcaag gattctgagg agaaatacat ttgcttttct ccttctgtta
     4561 ctgcctgttt cattgctcca atgatacttg caatctgttt cgaatttctg gcagatgatt
     4621 ctgctagttt acttacttca tttgcaacga tctcgaaccc ctttccttct tctccggctc
     4681 ttgctgcctc aattgctgca ttcaaagcta aaaggtttgt ttggtttgca atcgaattaa
     4741 tgatgtttaa aattttagat gcctcgaggg aagagttaga aatattttgc atttcattta
     4801 gagtgctatt taaatctttt tctccttttt taccaaattc taaagacttg cttccttttt
     4861 cttcaataga tagcgtccaa cgttttattt ggtctgtcat atcagaaaga ctagagattg
     4921 attccgaatt aatcacacaa ttctcatctt gtaactttgc catagatgca atcgaatgaa
     4981 ttgattttgt aaaattttca gttgattcta gcgactcacg tgcaaattga tattgatcac
     5041 ttgcaatcaa tttactctct tgtagtatat cgttaatgtc ttgtgcgatt tggtttaatt
     5101 cttgaattgc gaccgaattt ttttctgata attcttctaa ttcttttttt cggatatttg
     5161 ccttgtcttc tgaatctttg aagttcatga caacttttct gacaaatcta gttccaaaat
     5221 aagaggctat tgtcatagca aatataaaca tcattccaag tataattgta agagtagtca
     5281 ctggaaaagc tgccttgtct cctccttttg cttttattaa aaaacccaaa gtagtaaaga
     5341 gaagagtgca agaaattcct gtaaataaag taaaacgtgg agaaaatctt agaatggatt
     5401 cagctatcag taaaaagtaa atcccatata aaacaatatc gttaatcgca atcgtgagta
     5461 attcaggatc ttcaaatctt aagaatccaa ataagacaaa acccaatcca aagatttcaa
     5521 gtcccattgt gtaatattta acatttgtag tgtatttttt tgctctaagg atgagggctg
     5581 aagagacagt tgcgattaca tagatacaaa cacctgccac ataatttcca agtatcttag
     5641 aaacaaagtt ttgaacgaga aaacccataa gggtccctgc ggaaaaaatg aaaattagaa
     5701 aaagccgcag atagtttgct gccttttccc catttctttc taattctatc ttatgatcca
     5761 taatgaatta aacgactcaa gcactaggat tgtgagttct aattttaaaa caagtttatt
     5821 tccaattttt gaagagtttg aaaaactaga aaaattccta gtattatttt gctctttgtc
     5881 taatacaaat agcggaaaag gtttttattt tgagtggttt tctggaaatt aaaaatgcat
     5941 tcatttttta gatctcaatg gagtagatgg ctctgtctat ttttaatttt gagaatcaaa
     6001 agaaatacaa aggaaggagt aaaatattct gggatagggt aacttaaaga aaaaagatcg
     6061 atggttagaa ctccttccat tttatctatt tcgtcccccc taaattgata gttatttttt
     6121 tctgattatt agaaaaaatc atctctgctt ggtcgactta tactctttgg gtaagcgata
     6181 ggaatcaaga aacaaaaaat aagttggaat ttttaatatt tgatttggtt tttttctgtc
     6241 atggaaatga aatccggtat ctgggttaac tcattggttt gctttactaa aaaaactttg
     6301 ctccttgctt cgattcttct ttctattcgt taacgttagt tcaataaaaa gagttcgttt
     6361 tctaaaattt atagatttca atcaaaatgt ttcgtaatag taaaaatagt ttaccatttt
     6421 caatacttat attattatac atccaatgaa cgatagaaat ctagaaccag aactcgattt
     6481 atcaaattgt gaaaatgaac caattcatat tccaaataaa attcaaccct ttggttttat
     6541 actcgttgta aatccggata cattccaaat cgttcagtgt agtaagaacg tagataggta
     6601 tctcggatta tccccagaga caattctaac aaaaacactt tttgatctaa ttccaaccga
     6661 acaagttgat ccgattcaag atgtaatcat cactaagcgg gtaatctata ttaacccaat
     6721 agatcttatc tttttagaag aaaagatttc ttttcgatct actttgcatt taacagatgg
     6781 attattaatt attgacaatg aaccaattga tacatctact tttttagatt tttatagttc
     6841 tgtcaatcga ctcatggatg atctgaaacg ggcaaaaact attttagata tgagtaatct
     6901 gattgtaact catatacgtc agttgacaag atatgataga gttatggtct atgagtttga
     6961 caaagactgg aatggaacag tcattgcaga aagcctctct ggagatttgt attccttttt
     7021 aggtcagcat tttcctgcat ccgatattcc aaaacaagct agggatttgt atttacgtaa
     7081 tccaattcga atcattcccg atgtcaatta tactcccgtt caaattatac ccgaggtaaa
     7141 tcctataaat caaaagttca ttgatttgtc taagtcttca ctcagaagtg tatcgccaat
     7201 acatatcgaa tatttgcaaa atatgggcgt taaatcaacg ttagttattt ctgtaattat
     7261 aaatgatact ctttggggtt tgattgcctg tcaccattat gaaccaaaat atatagattc
     7321 aaaaatgcgc aatatgcttg aacattttgg aaatatattc tcttataatg ttgatatatt
     7381 acaaaacatt cgattgaaag atatcgatat aaaatttaat tctattcaaa ctgaattaat
     7441 aaagagacta aaccaagaaa aggatttttt tacaggctta tcaaaatgta tgcctatact
     7501 cttacaggta aattctgctg ctggagttgt attgtttttt ggagataagt cttttaaaga
     7561 aggaattact ccggatagag atttcacaca tcgcttaatt gattggcttt atttaactca
     7621 ctcggaggca attgtttatt ctgattgtct cagtgatatt tataccgaat cagaatccta
     7681 tatcccgatc gcaagtggta tacttgctat gaggctttca aaatttgata agaattatat
     7741 aatatggttt aaacctgaaa tgattcaaag tgtgatttgg ggaggaaatc ctaaagagaa
     7801 aattattgcc attccaaatc aagatggttt tagattgagc ccacgaaagt ctttcgaaaa
     7861 atgggaagaa gtcgttaagc gccattcaac tccttgggaa aaatcggaaa ttcttttcgc
     7921 agaaaagctg agaaatacat tccaagaatt aatagcaaac cagattagta ttctagaagc
     7981 gcaaaatatt gagcttggaa ttataattca agaaaaagtc ctagaaatta aaaaaacaaa
     8041 tagagagctc caaaaagtaa gtgaacagat tcggcaacag aatgaaaaat taaaaattca
     8101 aaatgaaaac attctaacct tataccaaga attaaaaaat cataagaatc aattaaaagc
     8161 tatttttgat aatacgaaac acatcatatt tttattggat acagaaggta gacttctttt
     8221 ttttaatcag gtcgcaaatg aaatttcttt taagctacat ggtagaccac ttacgatagg
     8281 agatacatta aagagttttg caagaagcca agaggaaata gaccggattg agaaacgatt
     8341 ccagaaggcg atgagtgggg aagggtttgt tctagaggaa gaggtcttcc atgaaatgtt
     8401 taataaatca gcttggttta ggattgaata tgacccagta tatgaagaag atagtctcat
     8461 tggggttgcg attttaatga cggatgttac agaaattaaa atggctgctc aaaaaataga
     8521 gaaccaaaat tctgcactta gagagattgc ttttttacag tcccatcgtg ttcgtagacc
     8581 cttggcaaat atactcggac tcatacatgt ttttaatcag gaagatctaa gtgatccatt
     8641 taataagact gtattagaaa aattactttt cagtactcat gagttagatg aagttgtaag
     8701 aagtatcgta gataggacat ataattttga tgaatagtat tcatttactt ttgaaagaaa
     8761 acacagatga tcttcacact aggcttgagc aagttggatt tgtaaataaa atcatgaatc
     8821 aaactttaac tcttcaggat tataagaaac taattcgtgc aaattatgca tttcatgatt
     8881 ctgttgagag aaatctacgc ggtataatgg aattagccga tttagatata atctggaaaa
     8941 tgccctttct agagaaagac cgttttgcag taggcgattt tataatagat aaaattgaaa
     9001 atccatttga accggaaaat ttttttgaag ccttagggag tctctatgtt ttagaaggag
     9061 caactcttgg aggaaaaacg attcgacgtc agttgcaaaa tcttcctgtt aacatgcatt
     9121 attatggctg ttatggggaa gatgggggat ttcactggag acgatttcta gatattttaa
     9181 aacgaaatgt cgatacagaa gaaaaagcaa actttgcttt aaagggagcg aaagaaactt
     9241 ttttatttgg gattagatgc ttcgaaaaaa tctgccaatt ataataagat ttttattcca
     9301 agaccaatca ggataactcc accaacgatt tctgatttct tgccaaattt ctcgccaatt
     9361 tttttaccga caaatccacc cgttagagta aaaagcaaac aaatcaatcc tacaaagata
     9421 gttgtcaatt tccaatctaa ttgtaactga gggagtgcaa atccagcggc taacgcatca
     9481 atacttgtag tgatggagag caaaagatat tctttcgtgt tatccggtaa atcttcttcc
     9541 tcttcctctt caaaggattc tctcaccatt ctaattccaa tccaaagaag taataaaaaa
     9601 gaaatccatt ttgtgatagc ctcaaaagat tcagataaaa aattccctga gaaatatcca
     9661 attactaaca ttccacattg aaacaatgta aataaaaatg cgagagttag aatacctttg
     9721 agtctgtctt ttgaattctt gattccctgt gaaatggata tcgcaaaaca atcgagggaa
     9781 agactaattc caattaaaat tgatgcgatc attttttaat actattttga attaagctct
     9841 cttgtaaata aaataaaaaa tggaataagt aatttaaact taaaaaatac gatatccgca
     9901 ttaggattca ttgcttccta tgctaagtat taaaagaaag atttcgattt tatacactgc
     9961 gataggtgtt cgctcaattt ggtttgacaa taatagaatt aatatcgtga tctagtaaag
    10021 ataaatcatt gctaggaaaa tatttttttt aaatagatcc ttcgaatcaa tgggtggaat
    10081 agtttgatta aaattttacc aaatataaat tactaagttt gacaggagag atattttttc
    10141 tgagattgaa aacacgatga ggttttatgt ctgaacattc taaaggttta tctaatcctg
    10201 ttatgacaac tattgtcact tgtcctatct gttttcatga tgacgtattt ttttattctc
    10261 taaaagctaa gtcattagcg agtagacaaa acgtttttag tattccaatc tatttagata
    10321 ctccaaaata tgtatatgta gattttaatg attattcttt tagtgtttgt ccgcattgct
    10381 attttacaag caatcgaaag caggatttta tttatacaga ttccattgct ggaacaaaaa
    10441 ataaatccac tttacttccc aaaatcattg agcattggac cgaaaataag aaggaagttg
    10501 aggatttgct catcgataat tttgtggatg aaaattcttt taaagaacca agaacagatg
    10561 aaggaattat ttcaagtagt aagctcggaa tctataaaac aaatctggag atactttata
    10621 aagttcccta ttctcattta aaaagagctc gcgcttattt aaaattattt tatacaattc
    10681 gtaaactata taaaaatgaa gacgaatcga ttctaaaaaa agcgttagag gatttggagt
    10741 atgtatttaa agaatctgat tttcctgaaa tttcttatga atacgaagta ttgtatttaa
    10801 tctctgcaat ttatatgaga ctcggagatg aagcgaatac aactcaatac tttaaagtat
    10861 ttgataaaac aaaatctgaa gtactacaaa aagccaaagc caatccccaa attaataccc
    10921 atgaagtaac acattggttg aatcgagcaa aaaatctctg gcaagataga agtgataata
    10981 tggtatggga agttttaaat ccatggccac gtaaatagga aaagatatgt taatcaaaaa
    11041 aataggaatt ataggagcta ctggaatgct tggaaaaccg gtggtaaaag ccttattagc
    11101 cgcaggcttt gaagtatatg caatggttcg agatataatc aaggcaaaaa ttgaacttcc
    11161 aattggggta aacctagtag taggagacat cggtaatcct gtcgagattg aaaatttttt
    11221 aaaacaggta gaagcaattc atctaaattt atctataaaa caaacagaat tagagaaaga
    11281 atttcatact gaagaacaag gtttaaaatt acttttggaa attgctaaaa gaagaggaat
    11341 caaaagaatt tcttatttat cctctcttct tatgaattat cagggaacta acggttttca
    11401 ttggtgggtt tttaccataa aacaaaatgc tgttcaacaa attaaaaatt caggtattcc
    11461 ttatactatt ttttatccat ctacatttat ggagaatatt ctccatagac aaatcaatgg
    11521 tgataaattg cgattggcag gtctttctcg ttataaaatg ttttttattg caggcgatga
    11581 ttatgctaga caagtggcaa actcctataa aattttgaga gcggaaaatc gagagtatcc
    11641 ggtgcaagga ttatttcctt atacttccga tgaggcagca actctcttcg cgaaatttta
    11701 taaaaaagag attttgaaag ttcagaaaat gcctatgttt ttattatctt ttttatctag
    11761 atttagtcca aaatttgatt atctatataa aataatatat tctatgaatc actatcacga
    11821 aaggtttcaa tcagaactta cttggaaaga attaggaaaa ccaattctta caatagaaga
    11881 gtatgcggct aatcacaaat agccgaagta agttcgattg atttcatttc ctgcattgat
    11941 taaatcattt ataaaatttg tcagatactc atgtaatcca aaaaagaata tctcttccgc
    12001 agaactatag gctatttgat aatatagttt tccaattttt tgttctgctt tatttgcata
    12061 aaatttatca ggaatagagg aaatatttcg aaaagattgt cttaccctct ccaagcaaaa
    12121 tagtagagat cttggaagct gtggattaaa taccaataat tccactattt gaattggagt
    12181 aatcttaata ttatagtgtt gtaaatatgc ttcataagct cctgtggagt cgagtaacgt
    12241 tttccattgg taaatatcaa gaggatttcc aacatcctct actctcggaa gtgggatatg
    12301 atacttaacg tctaggatac gcgcaatctg atctgctcgt tctagatatt tccccgctcg
    12361 catgaagtga tatcctttcc ctcggaacat actgatttca gttgcacctt cgaatagata
    12421 actcctttga cggataaaat taaataattt atctggtcct tcctttcgaa ctaaatccat
    12481 attaaattga tttagttcgt ggtaagtaac gttgataatt tcccaacttt cctttgggat
    12541 catgtctcgc acacctctgg cattttctct tgcacgtgct atacattcca gaatagagtt
    12601 tggattttca ggagaaaaaa ctaaatagtc aatcaccttg tcggatgtcg cttctgtatg
    12661 aattttagaa aaggaactta gctgcccaac agcgtttaag attggttgcc aattattaaa
    12721 tccatagata tccgattcga gagatgcgga atgttgaact tgtaaactga tggaaatacc
    12781 ttccgcccgc tcaataaatc ttcctaacca aaaaagtgat tctgcaattc tacttaacat
    12841 attaactcac tacccaggtg tctttgcttc ctcccccctg agatgaattt actactagag
    12901 aacctttttt taaggctact cttgttaatc ctccggggat cagttttatg ccatttggag
    12961 aatagattgc aaatggtcta agatcgatat gtctaggctc tatcgttcca ttaaaaaaac
    13021 aaggagcagt agacaaattg atagttggtt gtgcaatata atttctggga ttttccatta
    13081 ttttcttttt atatttttct atctcttctg gagaagcatc tcttccaatt aacattccgt
    13141 agccgcctga ttgatttgtg gcttttacta caagtgtatc cattttctta aatgcttctt
    13201 ctctttcttc ttcaattccc attctgtaag taggtatatt tttgagaatt ggtttttcat
    13261 ttaaataata ttgaatcata tctggcacat aggaatatac ggctttatcg tctgccactc
    13321 catttccaat ggcattgact aatacaacgt ttcctctttt gtaagattca aacaatccag
    13381 gcacacctaa aacggaatct tttctgaata ctagaggatc tataaatgag tcatctactc
    13441 ttttatagat aacatctact tgtttgagac cttcaattgt tttcatataa actttgtaat
    13501 ccgatacaat taaatcataa ctctctacaa gttgtattcc catttgtgaa gcaagaaaaa
    13561 tgtgttcata gtaggcagag ttgtaaactc caggtgtgag taatacaatc gtaggttttt
    13621 cttttccgaa gggagctaat tgcataagca tatcatgcaa taaagctgga taggaatcca
    13681 ctcgaagcac agagctttcc ttaaaaatct caggaaagat tctcttcatg ataattctat
    13741 tttctaatac ataggatatt ccagaaggag ttctgagatt atcctctaac acatagtatt
    13801 ctccatctgc atctcttatg atatcaattc ctgaaatatg tgtgtagata ttatgaggaa
    13861 catgaacacc taccatttct tggatataat caggacaggt gtaaaccaat tctcctggaa
    13921 ttattttatc tttaataatt tttccggagg agtaaatatc tgttaaaaag agatttaagg
    13981 ctttaattct ttgttgtaat ccttgttcga tagtatccca ttccattttt cgaatgattc
    14041 gaggaattaa atcgaaagga aagattcttt caataccttt cccatcactg tagacagtaa
    14101 atgtaattcc ctggttaatc gaaactaatt tagccagtac ttctttttgt ttgaactcgc
    14161 taattccaag ggttgcaaga atttcaaata catctttata gtgttctctt ggcttatcag
    14221 ttgtagtaaa catttcatca tagataggat tggtctcatt gtaatctaat ttgatattca
    14281 taattgtttc ctttttaggt gcttaccgat taagcaaaac gaatcgactt gctagatttt
    14341 taagaataat tcgctagttt ttaaagcttt tagtcttaat ttgccctcaa cgtctaatgt
    14401 gcaaaattaa gaccaaaaaa aatatgaatt gcaaaattct tgcattaaat taccctttct
    14461 cgccaaatta cagaatgaaa tgcttgtgat ttttgttctt tttttttatg gtatatgatt
    14521 tgcaaataga agacctgaga tgttgataga agagaaatta caacaaagcc cggaagattg
    14581 gacgaagtgg ttagcacaag gaaatgtggt tccctatttt caacctatct tatccttaga
    14641 aaataaatct gtcttgggat acgagtctct ggctagaatt gtcttctcag atggaacagt
    14701 aaaaacaata ggagattttt ttatttctga attcaatgga agtttagata agcaaagaga
    14761 atttaaaaaa ttccagagac ttgtagacca tgaattaaga aaacgagcat tgtatttgct
    14821 cacccatgac ccaaatccaa attcaaaatt gttcttaaat atttcccccg cgattatgct
    14881 ttcctatttg gatgaaggta attctgaaaa tgaattacaa gcctttattc gaaaggtaaa
    14941 tgaacttggt atttctccag atcgaattgt attagaaatt acggaagact atatacataa
    15001 aaatttagag ctattaaaac cattgattgc attttataaa gacttcggat tcttaattgc
    15061 aattgatgat ttaggctcta agtcatcaaa tctagataga atcggtatat ttcaaccaaa
    15121 tatcatcaaa gtggatatgc aaatgttgcg tatgtcgctt gtagatagaa gttataaaga
    15181 aattctatat actctttctc gtttgggaga gagtttggga atctcattgt tatttgaagg
    15241 tgttgagaca gaagatcaaa tgtatcaagc aatgagcttt ggttccagat atttacaagg
    15301 ttttttattt gataaagcac ttcctaatgt tcctgataaa gaaaaataca caaaacattt
    15361 aaaccatctt gtggataaat tttatgcgat gaaattggat tccattaaac agaaggtaaa
    15421 ttgggaaagt cgtgtagagg cattgattca aaaaatagca gatgagattc gttttagtga
    15481 agagggagcg attctttctt attcagaaat ttttacagta gatccaagta tctttcgttt
    15541 ttttgtgact gatctacaag gaaaccaatt atctcccaac tatacaaaaa agaaaaattc
    15601 ggaaatcatc attcaacgtc atgcgagaaa ttgcaattgg agttggcgcc catattttct
    15661 aaatcattta tatgaatcct atcgtcaggg aaatccttgg gtgatcagtc agccttataa
    15721 tgatataatg gaaaacctac ttttgcgaac tttttccaaa acttttaaag accgctttat
    15781 tttgtttatt gatgtattgt atgaggagta gaaactaaaa aagcctgaat tgatattgaa
    15841 aaaataccaa acaggcttct ctctgctaaa aagcagactt acttcttttt tttagctttt
    15901 gtagaggggg ttttgagttg ttttctcttt tggagagcta agtcgtgatg gaaatcacag
    15961 tatttataag gttgtcctgt tttaggattc acttttttta cttttgctcc acaagcaaca
    16021 caaagtcctt gttctcttct ccttttagca aatgctctaa ttctctctgt tgcactttgg
    16081 ttgtatctgc ttacctggat gcgatatcca ttaaataggt aattcccaat ggcttcatct
    16141 gatttatttt ttaagttttt ttctaatgtt gttaagtcat tagttgtaac ttttactggt
    16201 ttaggtgcca aattttttat cctcaaggtt aaattagagt ggtttatatt gattagccca
    16261 ctccgaagta gacaatatta aaactcgatg attaggcaag aaatattttt ttactaagtg
    16321 gaatatgggg gttaatagcg ttttgccgat caataatttt attgcaaaaa ataatgtagg
    16381 gtatacaagt cgtaatcctt acttaaaaga aagtagggtt atataagttt cgccatttat
    16441 cgtatttcat tgtttattca atatgatatt tatcatataa aataaaacgt tactctatta
    16501 cataactttg tcggattcaa atcaaaaaaa tcgtagtaga tttttaattt tattccgttg
    16561 ttctaggtag attagaccct tatttttact ggttcatttt acattcttta ttttcctaaa
    16621 tgcttgaatt ttaaaataat ctaaaactag tatagtaaac aatagaaaat tcttactgaa
    16681 tagatttcat atatttaaaa tatatttttc gatgacaaat atcgtattct tttcataaga
    16741 tttaggagca gaatcgatca atcaatatac ttaaaagaga gtagttgatt attaaatata
    16801 caatttttat tttatatata catagttcca tttgtaaaat tgatattatt tatttacaac
    16861 agacacaatt tacaattaat tcgatttcta attacctata tgtttaacta tttagatttg
    16921 aaatattttt aatccatttc tatttctaat tcaagatacg atagtgaaat gtttggtaaa
    16981 aacaagattc atttttatga aaatatttta gagaaagcat tggtgttcta aatttccttt
    17041 tagaaaatct gctacaaatt ttgcgagtgg ttcttgagtc acattaaata atcaatccga
    17101 agaatcgata ccagctgaca taggaaacat gtaaggattt gatttgagta gcatttggga
    17161 tactgcacat tgagagcgtg ctagagcatt aagtagtgtt aagagtgaat aattaattcc
    17221 caatcaaaaa taggaaatta atttttgcat gcagtataaa tagtcaaaag aatcagttta
    17281 gacggaagaa ataaaataag gggactagat ctagaaaatt taaatttttt cttcgattat
    17341 ttattttttt aagtcccaag gtttgtcttc attcttcttc cactttttta tatgatttga
    17401 tttagaaaaa tcttcaaaaa cccactagct gtttgttttt ttggaatcat tttcctatgt
    17461 atttttttgt agcagttttt aaagcatagg ttgagtctgt cagtattcag tattttaaga
    17521 gaggtcgaaa tgaggtctat cattgttatt ttattattta caattggatt tacatctatt
    17581 cttgcgaatg atgggaagtg tgagaatgga gattgtgaca atggacgggg agtttttatt
    17641 ttttcggatg gaagaaaata tgacggtgag ttcaaagcca aggaaatgaa tggtctcgga
    17701 attttatatt ttaaaaacca aaatgtgtat gaaggcgatt tcctaaaaaa taaaatagag
    17761 ggaaagggac gttatacatt tgccaatgga gatatttaca atggggatgt aaaagatggt
    17821 aagtttcatg gcagaggaat tttttataca aaagatggct caagatactc cggtgaattt
    17881 gttgaaaata aatttcaagg gataggtttc tttcaatatc cagaagggca actttatcta
    17941 ggtaacttta aagatggaag acctttcggt ttgggaattt ttaaaaatag tgaagattct
    18001 cgtttagccg gtgatttttt agataataaa gctatttcac aaggatttgc cattagcctc
    18061 aaaaaagata aaagcttcag taatattgaa aatggtcaac ttctagaact tagaaaatta
    18121 ctttataaaa atcataaata ctatgcaggc gaattgaaag aaggaaagtt agaaggaaat
    18181 ggctatttaa tttcagagac aggcaatgag tattttggaa attttaaaaa tggaaaatta
    18241 catggatttg gatttttaat ctcttccaag ggaaattatt atattggaaa tttcaaggat
    18301 ggaaagtttc acgggaaagg agaatttttt aattttgcag gcgaattaaa agttgccgga
    18361 gaatgggaga atggtaagca gattccttct gtgaattcaa atcgttaatc atttgactcg
    18421 cttattttaa gtttcagatt ggttttatgt ttcgagtatt tatcttaatt cttttctttt
    18481 tttcatttaa cagtggattt ccgcaaagtc aggattttag ttttatttcc tatggcaatc
    18541 tccccacttg gacaggagtg ggggtcgatt ggagctcatc tcctaaagtt catatttatg
    18601 atacaaagat ttatcagggt catgcagaat ttgtttctcg tggacttgga agaaaaattc
    18661 gttttaatga ttttaaaaaa cttgcaagac aatctcaaaa tagagaattt ctgcctttct
    18721 ttttatatga tttaagctcc aaacctttta taaataaatc aggtagtttt aattgggcag
    18781 ttcgattaga aaattataac tacgatgata aacctgcaca tcttgctacg gaaattctaa
    18841 aactagtaaa gatgatttct gaattggata aaactttcaa tgggaaaggt ttaattgttt
    18901 taacggaagg tgataataat ccgttaggcg ttagtaagct tggaaaattc attctaaaag
    18961 aaggacattc gtttattaca ctcaatgagt taattaaata cgtgggtgga aagaaaatgg
    19021 aggttttaaa tcctggtgtt gcttttggtt tagtaaagtt tatcaaaaat gaaaaagaac
    19081 tagaagcgct tctaccaact gatattgcaa tcctaaattt tattcccctt catattcctc
    19141 ctgtttcggg aattgtatcc ctaaaaccac aaactcctct ttcccatgtg aatcttttag
    19201 caaagaatcg tgggacattc aatggatatg ttacagatat attcgctcta caaaatttaa
    19261 aatctctcat tggaaaatat gcaaagctat cgaataacgg agataaaatt ataataacaa
    19321 agagctcaaa aaaagaagta gatgaaaatc agaagttaaa tccaagaaaa caaattataa
    19381 ttccagaagc tagacaggat ttaactgaag tcattagcct tacaaaagaa aacgaaaagt
    19441 ttattactac ttctcatatt ggagcgaaag cggcaaatta ctatttgctc ttacagaatc
    19501 ttcctgattt aactagacct gcttttgcta taggatttaa gtactacttt gatacaatca
    19561 aaggagagcc agagactatg attcaaaatt ttctgaaaga gaaagataaa ttttcaaaag
    19621 aagaaaagtc aaaagcatta ttaaaaatta gatctgcaat tgagaaatca aattcatttc
    19681 cagccacact tgttaccagt atccgaaaga ttttagaaaa atatccacca aaaacaaaaa
    19741 tcaggctcag aagttctacg aataacgaag atttgcctga attcaatgga gccggtctct
    19801 atgaatcgaa gggatttaat tcaaaagaca atgatgaaaa gttaaaatct aaaatactat
    19861 ccgtttattc ctctctttgg tcagatcgag ctttttttga aagagattat tttggaattg
    19921 atcatagtaa agtaggaatg gctattttaa ttcaggaagc ctttcaaaaa gaagtggcta
    19981 acggtgtaat tgttacaacg ggtagtgata aaaaagtgca gatgattgta aatactcaat
    20041 cgggggaaca ctctgtcaca agcccagaag aaaatatttt atctgaatcc tttttattaa
    20101 atccagaatc gggaagtatt gaaaaaatat attctgaatc gagtctatct cctgttttta
    20161 ttggaactaa gacaaacgaa ttacaattaa aaaaaatcca agaagcaact atgaaactgc
    20221 attctatctt aataaaacaa aggatagatg caggagataa aaataaatat ggaatcgata
    20281 ttgagtttaa aatcatgcat gaaaaaggaa aaaataaact ctattttaaa caggttagac
    20341 ttttgaagta ttaataccta aggtgcttaa cggttactcc attattaatc aattgtttta
    20401 gagagtctac acccacacgt atatgtaaat cgacgtaatc gttagtcacc tctttatcgc
    20461 tttcatctgt tttgattcca tctggttcca tgggctgatc agagacaagc aagagagcgc
    20521 ctgctgggat ttcattgtag aaagctgtag aaaatatagt agccgtctcc atgtctatcg
    20581 ccataactct aatttttttt agatattctt tgaagtcttc atcccattcc cagactctac
    20641 gatttgttgt gtaaacagtt cctgtccagt agtcttggtt gtaatctcta attgtggtag
    20701 aaattgcttt ttgaagagcg aaagctggaa gagagggaac ttctggagga taatagtcat
    20761 tagacgttcc ttcgcccctt atagcggcta ttggtagaat aaagtcacct aactcatttt
    20821 ttttcttgag accgccgcat ttgcctaaaa ataaacaagc ttgcggtttt attgcactca
    20881 gacaatccat aatcgttgct gcgttagggc tacccatccc aaaattaata atagtgattc
    20941 cttctgctgt agcacttcgc attggtctat ctgctccttg gattggaaca tcatgccaat
    21001 gtgcaaattt ttgaacatag ttatcaaaat tagtaagaag gatatattgt ccaaagtctt
    21061 caaggttaac gcctgtatac cgcggaagcc agttttctac tatttctttt ttggttttca
    21121 tggtttgtcc tttattgcat ttcggattgt agtattttat agaaagactc aggtatagtg
    21181 agttttgaat ctatgcggta gtcgtaatag acctggacag tttttgctct ggcatagatt
    21241 gtattattga ttggatttat tatttcagct gaaaaatccc aagaggtatt cccaattcta
    21301 gagacccatg ttttgacatt tgcatcttct cctgttcgaa gagacttaag tatatcaagc
    21361 tctactcgaa ctaatacaaa cggtatgtct tcaagtgttt gaatctcgag atatttttta
    21421 caaaaatcca ttctacctaa ttcaagatag gaggaataaa ctgagttgtt gactcgcctc
    21481 atcgcgtcta aatcgctaaa tcgaatttga atcggtgtaa ttatcatttt gaataaattc
    21541 tatttcttcc atcttttttt gcttggtata agagttcgtc cgatctttga agcagagtat
    21601 ctggtgaatc tttatcggat aacagcgtga aaccaccaga ggctgtaact ctccaaggaa
    21661 ctccaataaa ggaatgcgac tcaattacag ttcgaattct ttgcattaca atatttgcac
    21721 tctctagatt ggtttcagga aataaaataa taaactcttc tccaccgaag cgaaaaacta
    21781 aatcagttga tcgaaccgtt gatttaatta tctttgcaat ctcttttaat acatcatctc
    21841 ctgtaagatg acctagagtg tcatttattt ttttgaaatg atcgatatct acaattccct
    21901 ggcaaaaagg atgagagttt tttcgagaga gcagtatatt gtggacaagt aaatccatca
    21961 tatagcgtct attgtatact cctgtcagtg gatcttttac agatatggtc tcaaggattg
    22021 tatttttgcg tctaagctct tcttctaaat gaaatgtttt tcttctagca tattcgatag
    22081 taagagttgt catgaatcca acaaaagtat atagaaaaat ctgtgtaatc atgagtccat
    22141 tcgttcttgt aggatgccaa atcgcgaggc aaattataaa tgcaactaga atggtaatat
    22201 aaagaacaga gtaatgagga attctagttc taaggataag tgggattgac atcactgcaa
    22261 atgcatagag agaaagatct tgaccatgat ataaatttaa gatatttaaa aacagtaagc
    22321 caataataaa aatatggaaa tagagaaggt aaataaagtg tatccgcaag tgattccttg
    22381 ttaaaaaata cacaagaaag gaaaatagaa ctgttgtaat tataagaaag ctataagtga
    22441 ttacatagat tgggtaaaat actgaattcc tgtctacact tggataaaga atatgttgca
    22501 ctaaaaaact agtagatgcg atgaaaacaa caccggctac gcggggaaac cgattccagt
    22561 ttttcatagc attaaataaa atatatttat cgatttgaat tcctcttaat tgagaaagtc
    22621 ttcttcgtat tatatttagc aaatacttca tgagattaga accgttatac acagagatct
    22681 attcgatttg aaagaaaaac tagggcgatt cgtccgatgg catttttgaa atcttataca
    22741 ttgcatccta cttctttcct cttctcgcaa attcatctag tttgtcttag agtaaaactg
    22801 ctttttttgc agtcattact tttttttcta tttctaatcc ttgttttcct aaaattcgtt
    22861 tgaaatcttt tttggaaaag gattttattt ttttatagca ttctttatag aaatcttctt
    22921 cttttccaaa ataatttttc catatagact tataaaaatc ttctgatagg aattcttgct
    22981 tctgaaaaga tctgttctcc ttctcaggat ttaagaatcg aatttgttcg gatatttcga
    23041 taggtgtaaa tttgaattgt aaaagcgctt cttctaagag agccatttca acataggaaa
    23101 tataatcaga caactctccc caaaaatcaa aaccttgggt tccataacta cttaaacaat
    23161 gaaaactaat acaagtagaa tttcgcgctg accagattcc acagtttttc ttctttcggt
    23221 cgaaataagg acagagccaa tcttctctat ttccaaactc attttttttt cttttggcaa
    23281 aatcaaattg atgtggaagt ggtgctgtga ttccaattgg aaatacatat tctctatttt
    23341 taattttagt ttgaattctt tcttttattt cttttggagt tcttgtagat aataaaactt
    23401 gtccaatcga ataatttggt tgaaaaggat gaaatgtgca gcattttaaa tgctcttcat
    23461 aacttctatg atcaggactt cttcctgcgg ctaatgcaca tttatcgcaa gtagcttttt
    23521 tttctgtgat ctcaaaatcc aaaagcgaat ctggaaaaag ttcttggtag agggttggaa
    23581 gaatggattt aagcatttgc aatagcctca gctgcaatcc aagaagttgt ccatgcattt
    23641 tgaaaattaa atccgccggt cactccatct atatccaata cttcacctgc aaaataaaga
    23701 cctttacaaa ttttactttc catagttcta aagtctactt cctttcggct aacgccacca
    23761 caggttacaa actcttcttt gaaaatggat ttaccggata cttcatatat accgttagca
    23821 agctcatctc ttaaaaaatt aatttctgca ttcgaaattt cagaccatct tttgtctgag
    23881 aggttcaatt tttttaaaaa agattcccaa agcctcgaag gaaggtgaaa cagggagaag
    23941 gatgatatct tttttgcggg atgttctttc tttgtttcta gaattttatt ttgtatttca
    24001 gaatttgtat ttggatagac ccagttgatt ttgatttttg ttttatagtc taaatcaaaa
    24061 aaagtcttag cttcataagc agaaagtttt aaaattgccg gaccactgag cccccagtgt
    24121 gtgataagaa tgggtcctgt ttgggatttt cctttaggga aaatagaaac ttccgcattt
    24181 ggaacggata atccctgtaa ttcgctgatt ctttcatcgg taatatgaaa ggtaaagaga
    24241 gaaggcactg gtttttcaat ggtatgtcct aatttttcta gaatttccca taccttccta
    24301 cttgagccag tggctacgag tagtttgtcg aaatagaact ctttctcctt tgttataact
    24361 ttccatttat tttcttcaaa gtaaattcca tttacaaggg atccgagttg aatatttact
    24421 ttcgcgcgat ttgcttcctt ttgaaagcaa tcgatgattg tagaagattg atttgtagtc
    24481 ggaaacattc ttccatcatc ttcttttttt aactctacac ctcttttaaa aaaccattct
    24541 attgtgtcct gaggttgaaa tctttcaaaa gctgctcgta attccttttt tcctcttgga
    24601 tatttttcgg aaagggaaac aggatcgaaa agattgtggg ttacgttgca tctaccgcca
    24661 ccggatattt taactttagt taaaggctct tctccttttt ccaagagagt aacctgagct
    24721 gtatttctta agaggttacc gatttggatt gctccaaaat atcctgcggc tcctccccca
    24781 atgattccta tttgtttcat tgattctcaa tatgaaatta gaaacaattt tgtaaagaac
    24841 ttgtagagat tgaattttaa tattacgcat ctttgcttaa gaacaacagg tgtttatctc
    24901 taaatgagaa atatcataaa aattaaactc aaactgaatg gataaaaagc aatagaacga
    24961 aaagatgctc ttattatgaa attaatgtac actgaatcgc aagataaaat gactagcctt
    25021 cgactttttt gggaggaaaa tcattttcaa ttagggattc cccttatcga tatccagcat
    25081 ttatggctgc tttatatcat ctcaaattta gaggcagcac tctcttactc caatacttta
    25141 aatgagacaa tgaaggattc tattgtgggt gtcattgatt atgtatctga gcattttagt
    25201 ttggaagagg aaattttaaa agaatttaaa tatccccaat ttaaagacca tgttgcacat
    25261 cacagggaat ttgtggaaac tcttcaaaca aaacatgatc ttgctaattc aaatgagtta
    25321 gccgctttag gtttgctcaa tatgctacaa aagtggttat ttaatcatat tttgaaagaa
    25381 gataagcagt atgtggaatt ctttaaaagt aagtcgatag atgtaagacc tttttgtcag
    25441 gaaattttaa ttcagaaaaa atataatatc acaaaaaaac aaggagatgt atataaagaa
    25501 gtttgtagct ttagacaatc cgaaattgaa gaagtgatca gtgaaaatat gatcgaagat
    25561 attatgcata tctggagatc acataatctt tccttacata taccggttat cgatttacag
    25621 catatatggc tactgaaaat ggtaattcaa ttagataaag cggtaaagac catgggaaca
    25681 acgaagaaaa acgaagtctt taatcaagtt gtagaagaag cgattgatta taccgtaaaa
    25741 cattttactt tagaagaaag aatcatgaag gagtttcggt atccagaaat actgaatcat
    25801 tttcatcagc acgaatcatt tgtagatttt ataaagcaaa gaagaaagga aaatgaatct
    25861 ggtgatacgt tagctgcttt tcatttagct caggatttaa agacatggct actttcacat
    25921 attgcacatg aagataagaa acttgccatt tatttgaagg ataaaatgaa agatgtaaat
    25981 gagttcacaa gaaagcttca ccacaatgga gaaattataa ttgagccaac acaacgaaat
    26041 ttctacaaga aaattacgaa ggatacttta taactcaatt tagattataa ttgcttaacg
    26101 aaaggtagga ttgagtttat aaaatacccc agtccgtgca aagtttttta aaaagggaaa
    26161 aaccagatat atctagatcg gttgttttgt aatgaagttc tttctttaga tactcttcaa
    26221 ccatttctct cggaaatctt ttttctgttt caataatttc accgatatgc gatattccat
    26281 actctagtga ttctaaaaat atggaatctg gatagctaga agatttcgga aaagcccaga
    26341 aagcgaatat gaaagagagt ccagttgatt tataccacca ttctgccaag tcatagactt
    26401 catagaattc tgaattccat tttgcaagaa gtgcgttgtc tccaaaaagt aaatgggaat
    26461 tgaacccatt ctcgatttgg gttaaaattt cttttggatc tgtggggata acactgatta
    26521 gctgatttgt ttcttttctg agaagtgtct gtaaaagtgc gacactactt cttgatccag
    26581 agtcagtata aattttttcg gaggggaaaa tttctttttt atttttgaaa aataaaatcg
    26641 atcgaacttt ttcttttgca caaactccac aagatgtgga gaaagataaa acagattcgt
    26701 ttcgaataca ttctacagaa gaaatgagtg ctaagtccaa ttcttttttt aaaagtaagt
    26761 cttttaggac agacggattc tcaaaaacga gagtatgatt tcccgttttc tcaaatccat
    26821 aggtaagtgg tcttgcattt aaatgcttca caattccaat tttcataaga ctataattga
    26881 gtggactcat ttaaagaaaa gtaagttttc ttctcaatag aagtcttaag gagtctttct
    26941 aggcgcatat caagatgttt tagtttttaa tactacttat gtgaagtagc atttaggcaa
    27001 agaattttta atcacattat tttgcgaccc cgagaagcaa ctttgagttt aggtataaag
    27061 attcgcattg caataccttt gtatttcttc gtgaacttcg tgctcttcgg ttcatctctt
    27121 ttattcttga cccatgtgat gaatcattgt tagtgtaaag aaaaagagga tttatgataa
    27181 ctggaaattt tttcttaaac aatgatgatt tattattaca ctttcataca ataacagatt
    27241 ggaaagaatt agtcgatgcg tatgagcatg gatttaaaga cgcagcagaa tttaaaaaaa
    27301 caggtaacga caaattggcg tttgctcctt ctacaactga agaagcggtt gaatattaca
    27361 aatcaactct agagtccgtt ggagatttaa ttggaaaaca acttgcacct aaagtccaag
    27421 aaatggataa gataggttta aaatacgata atggaaaagt aacgtttcca aaaccgatgg
    27481 tagatgctat gaatgctgtg atggaagcag gacttcttcc ttatgcgata ggcagacact
    27541 atgggggact tggaatgcca gtcacagttc aatcgatggc aatggaaatg gtttctcgtg
    27601 cagatggagc ttttggaatt gcactcgctt gttttaatct ggcagagacc ctagaaaaat
    27661 atggatcaaa agaacaaata gaaaaatatg ttcctctcat ggcagaagga aaatactgtg
    27721 gggcaatggc tctcactgaa ccagattatg gatctgatct tccaaatctc caaaccaaag
    27781 caacaaaaga tgcaaatgga caatggagaa tcaatggtgc taaacgtttt attacacatg
    27841 gttgcggttt tggagacaaa cagtcgatta tcctcactct ggcacgcaca ggaagtccaa
    27901 cgagtggggc aagaggactt tcttttttca ttgtaaagac tcaagatgtg catatcgcat
    27961 ctattgaaaa gaaaatgggt cttcattgtt ctcctacttg tgaagtagta tatgaaaatt
    28021 caccagcaga actaattggc gttgaagggc atggacttgt taagtattca atgggtatga
    28081 tgaatactgc tagactttcg atcgctgctc aatctatggg gatagcgtct gccgcatact
    28141 atgaagctaa gaaatatgca tctgagagag ttcagtttgg aaaactaatt caagaaatcc
    28201 cggctgttaa aaaaattctt acgcacatgg atcgcgagat tgtagcaatg agatgtattt
    28261 tactagaagc ttctcgcaca gtggatttat atctctggag agaagaacat ctgaaaaaag
    28321 atggaatgga cgaaaaagaa attcgtaaag atgagacaat ccgcttctgg gaaaaattag
    28381 ctaacctatt tactcctctt tctaaatatt atatctctga aactgcaaat acaatagcgg
    28441 ataacgctct ccaagtgcat ggtggatcag gatatacaga agactatgat gtttctaaaa
    28501 tctacagaga tgcgagaatt acaaatatct atgaaggcac aacacaactc caaatcgtag
    28561 cttgtatcgg tggagttgta gctggtatga caccaactgg atttttaaga gcttatttgg
    28621 acaaagaaac aagtaagttt tctccttctg aaaaatttct cagagttaga aagacttttg
    28681 aaaagattgt agaaacattt acgagtatta aagaaagttc tgttcgagat ggacttgctt
    28741 ttgaagtcgt agagtctgct gctagaacta tcaatggaat gttaatcgaa agatctgctt
    28801 ctcgtctaga cgatacgcgc tctaaagaac gactcctcct tgcaaaagaa tatcatatcg
    28861 atagcattgc aattcttgaa tcgaatctga ttcgtattca agagagggga gtaggctaag
    28921 taacgtcagt ttggaatagt agaagattac cacggatgca cacggatgaa cacagataga
    28981 cacatgcgga ggcgttggtc gcgaatgctt ggagcgaagg atgtagtaag cgcatatggg
    29041 atttactctt tcataaactg aagcattgta gaattgcagg tagcgaaaaa ataaatcata
    29101 aaaattatcg ttcccttatc cgtgtccatc cgtgtgtatc tgtggttaaa agccctaccg
    29161 cccatcgttc cgttatcggt gccctcggtg gtaatcagtg ctcatctttt tcaccgaatg
    29221 cttgcaatta caaactactc attgtaagtt gattcctatg caagataaga ttatagtttt
    29281 tcagaataaa aaaattcgta gaatatggca tgaagaggaa tggtattttt ctgttgtaga
    29341 tgtagtctcg gtattgacag aatcgaaaga tgacttaact gctcgaaagt attggaataa
    29401 attagctcaa aggctcagag cagagggaag tgagtcggtg acaaattgtc accaactgaa
    29461 actagaagca aatgatggaa agaaatatgc gacagattgt gcaaatactc agactttatt
    29521 ccgcatcatt caatccgaaa aagattttgc aattctaact tctgagatat cgaaagctac
    29581 atttggaatt accccttctg aatacaaaga actgaaagga ttaaaaaaga aaaatcaaaa
    29641 tttccgtgat cacatgaccg atctagagct tatcttcaca atgcttggag aaaaagttac
    29701 aactgaaatt tctaaaacag aaaaaccaaa cacatttgga aaaaataaaa aagtagcaaa
    29761 gcgcggcggc aaagttgcgg gtgtcgcacg aaagcaaacc gaaaaagaac taggcagatc
    29821 ggttattagt aaaaagaatt atttaccaaa gagaaaagat attcaccacg gatgaacaca
    29881 gataaacacg gataaagatt ttgaattatt taacccgtcc gtgtccatcc gtgtgtatct
    29941 gtggtaatct ttccctcgcc ttcactcgat aatcgccaaa agatattaac cacaaataaa
    30001 atgatttaga attataaatc tcatctgtgt taatccgtgt ccatccttgg tttaaaaaaa
    30061 ttcttaccgt ccatcgttcc cttatcggtg ccctcggtgt ttatcggtgg taatctttcc
    30121 ctcgccttca ctcgataatc accaaaagaa attaatcaca aataaaatga tttagaattt
    30181 ttaagctcat ccgtgtccat ccgtgtgtat ccgtggttaa aaaattccta ccgtccatcg
    30241 ttcccttatc ggtgccctcg gtgtttatcg gtggtaatct ttccctcgcc ttcactcgat
    30301 aatcaccaaa agaaattaat cacaaataaa atgatttaga atttttaagc tcatccgtgt
    30361 ccatccgtgt gtatccgtgg ttaaaaaatt cctaccgtcc atcgttccct tatcggtgcc
    30421 ctcggtgttt atcggtggta atcttttcct caacttcgct cggtaaccgc caaaagatat
    30481 taaccacata tttaataatt tagaattatt caacccatcc gtgtccatcc gtggttaaaa
    30541 aaaccatgtc ttgacaaaca aatccacatc taaacaatgt aggtatgagt caaaaacagg
    30601 taagtttaaa agaagggtat gtagtatcaa gggaattaga agaagcgctc cttgtagctg
    30661 aggtgacagg aagaccgctt ctcatcaaag gagagcctgg aacgggcaaa acactgttag
    30721 ccgaacatat ttctaaaaca agaaatcttc cgatttacaa atggcatgtg aaatcaacga
    30781 gtcttgcaaa agatggactc tatttttatg atgcggtttc tcgtttgaat gattctagat
    30841 ttagtgaagg aaaagacaga gagaaagttc ataacatcga aaattacatt cgaatgggac
    30901 cacttggtct tgcttttgag tccaaacaaa agtgtattgt attaatcgat gagattgaca
    30961 aagccgattt agaatttcca aatgatttac ttttagaatt ggatcaaatg gaatttgtag
    31021 tggtggaaac caatcgaatt gtaaaggcaa aagaaagacc actgacaatt atcacatcta
    31081 ataatgaaag agagttgcct gacgcatttt tacgtagatg tatttttcat tatattgagt
    31141 ttccaagtcg tgatttaatg gagcaaatca ttcgagtcca tctaccggat atcgactcaa
    31201 cacttttaca aacagcgatg aagacttttt acaaagtaag aagactagaa gaactaaaaa
    31261 agaaaccttc gactagtgag ttactcgatt ggattcagat tctactctac caaggagcca
    31321 aattaaatcc aatggataag attccgtttg tgggagcact cattaaaaac gaagaagatt
    31381 tatttcaata ctcaggacaa ttacacgtgc cacaaaaaga ataaaatgtt tactcaattt
    31441 ttctaccaac tcaaagcaaa aaaaattcca gtctctactg cggaattaat cgatcttttg
    31501 aaagcgattg attttttctc caacaaaaat ggatccttat ccattaacga attctattcg
    31561 atatccagaa actgtcttat taaagacgtt aaatactacg atgacttcga tcaagtgttt
    31621 gctgaagttt ttaaggggca ggtcacatct aattcagaaa tgaaatctct gattgaagaa
    31681 tggttaaaac aagccataga tcgagtgatt ccggaagaac aaaagaaaca agcgaaagag
    31741 ctaaattatg aagatgtgtt taaagaatta caaaaaagac tacaagagca gaaagagcgt
    31801 catgatggtg gaaataaatg ggtgggaaca agaggagttt ctccctttgg aaattctgga
    31861 ttcaatccaa atggagtgag agcaggtgga gattctagtg gcaaaacggc gtttgacgtt
    31921 atagatgaga gaaagtttag agactatcgc acagatgaga ctctcaatgt gcggcaaatc
    31981 aaagtagccc taaagaaact ccgaaatcta aaaaaagaag gacgactgga attttccatc
    32041 caaaaaacaa ttgataaaac aacttcgaat ggcggtgaat tagaattagt ctatgaaaga
    32101 tctagaaaaa atgatttgaa actcttactt cttatggatg tgggtgggag tatgacgcct
    32161 cacgcaaatc gagtgagtaa actttttagt gcaagtcatc aactcaatca tttcaaagaa
    32221 tttcattatt attactttca caatattatc tacgatgctg tctttactga tgcaagcatg
    32281 tcaaaaaagg tttctatctc aaagcttact aaaaaattta gacctgatac aaaagtgatt
    32341 tatgtaggag atgcttccat gcatccttat gaacttttta ataaaacagg aatgtatgat
    32401 ttttatggct atggatattt aaaaaataca aagccggaag taaaagtgaa aacaggattt
    32461 gatagaatga gagatcttac cgaatacttt aaagattcta tttggttgaa tccggatgat
    32521 gaaaggtatt ggcatcatga aacagtagag gctatttggg gtttagttcc catgtttttt
    32581 ttaagcattg atggattaga tagagccatt aaaaaacttc tcagaaagta ggggattttg
    32641 ctagtattgg agtcatcgaa aaattattga ctcatttaaa gatagcgata atcttaaaat
    32701 cgctttagga gcttattatg aagaatattt tacttattat tattttcttt tttagttttc
    32761 agttaattgc ctttcagact gtcagcgaag ccgagtttat tttagttcaa aaaaaatcta
    32821 ataaacaaga aagaaacaaa tctgagaaag aagacaagga taaaaaagat gaaaaggata
    32881 ttggaaataa agattccaaa gatactaagg aatctaaaaa tcctgaatct ttaaaagaaa
    32941 agatcaaaga aaaattaaca aaagagaaag aaaaaagaga agggggacga tttggcgaca
    33001 aacgtttcta aatttaaact aataccaatt tgttttattt tattttttgt aggaatcaat
    33061 tctctaattg cacaaaccaa agagaatcct ccttcagtaa agtatgcaat tgaatatatt
    33121 gatgctctcg atctatctaa gggtgactat cgtatggcaa ttcgaggaga aaacccagat
    33181 gagatgatag aaattctgaa agggaaatcg gatcatcatt ttagagtaga gattggagat
    33241 acaaagactc agatctatcc aggttcagtt tcacaggaaa gaagaaactt agtagagttt
    33301 tacttaaatg tgtatgataa agttccagca ggcaaatata aagttaaact ctatttagaa
    33361 gagactacaa attcctatcg cttagtggat caaactgatt ttgaaatgcc agaaaactca
    33421 gaagaggata aacagcctgt agtaacaggt ctttctccaa tgggaggtgt gatcggtgac
    33481 acgatcacga tgtctggaaa aaactttgga tctgatttgg acaaaattga tatttatttt
    33541 tatgatctag atgatcatac aattgatatg gaacttccgg aagatctaga accatctgat
    33601 taccagccat tggaatatat gcagatgcgt gcgaagattt ctccatttta tatatctagc
    33661 acgttagacg gaatgcaaca tgttaatttt acgattccta ctacgcctta tttaaaggaa
    33721 ttagccgaca aggcattctt tagaaagagt attaaactca aagtgtttgt aaatggaaga
    33781 cctagttcct ttatgaaagt aacgatctta ccaaagaact ggaataaaaa ggttattgct
    33841 cttgcgcttg tcgtgacaat tctaggtcta ggtttaattt ctttgatggt agggaaactg
    33901 aattttattc cctatgtgct cttggataaa aacacaaata catatagcct gtctcgcttt
    33961 caagctttta cctggacggt tgtattaact ggtagttatt tttatattgc aattgcattt
    34021 ggaatccttc ttcaaaatgg gagaattcct gacttcaatc cttctttagt ggggttaatg
    34081 agtattagct atgctggttt tatagcatct catttcctaa ataagaaaaa tcccaaaaat
    34141 gcgatatcag acacaccgcc agcgcttagt gatttgttta tggaaaacgg ggtaattgat
    34201 attacccgct tacagctttt acttttcaca gtggttgctg taattgtata tttatataat
    34261 ctctatttaa gtaatacttt aaatggactt cccgatattc cggcaacgct tcacgggctt
    34321 ttaatgtcaa gccaaggtgg ttatattggt ggaaaaatct ttggagaaaa gatagcagtc
    34381 aatcgtatcc ttcctcgaaa aatctcctct tctgataaaa aaatcgatct acatttgatt
    34441 ggagcgggat ttatcgaagg tatgaaagtt gtaattgagg gatctcatgc agaaccagtg
    34501 caagtagaat tttcgagccc ttctaccgtt agctgctctt taccaatgga aaaagaaatt
    34561 ggtatgagag ggattgtatt gattcctcct tccggatcaa ccattgtcat acccaatgca
    34621 atcgagattg ttaatggctc tgtaactgag gcggaaagtg aaaccgattt agaagttgaa
    34681 aagtcaaatg gagtaaaaaa gaaaaaaggc taacggttgt tagcctttat tttaaatttt
    34741 ccaatatttt tatcccattt cattggaatc tcacttcgat aaaagtattt catgaatgat
    34801 ttgcactttt tttcattcaa accatcacta tagcagtttt catccaatac aaaaaatgca
    34861 ttggttccaa attgaggcag ctcaatataa gttcttggat gaaaattgat ttcttccata
    34921 tttttgatta gaggataatc ttttttattt aaaaatagtt ttatatctaa ttcaggtagg
    34981 actttgtctg ttacttctat ccaactttgg ttttcataat aaaagaaatg gttttcatgt
    35041 cgattgctat ctccaccata ataaacatct actccaatca aatgtcttcc atttttttgt
    35101 ttaaagattg ttagagttat atcatcttgt tccattttga actttatata ttcattcttt
    35161 gtatcaaata taggaaatcc gtttgcaata caatctataa tataattaac gcttttgcaa
    35221 tttttatcat atcgaatctt ctgaaattct tttatgcgca gtgagtattt tttatctact
    35281 tcttcttcta aaattgggat actaggaagg agcaaaagat attctaaaat atttttagga
    35341 tttcttctga ctcttttttc atcttttgca tctaggattg tttgaaataa tttttcattt
    35401 atgttttgtc ggctttctag aatttctatg attttggaaa tcgattctgt atcataattg
    35461 tatttctctc cttctttgag tttttgttct gcgatttcta ttgcatttaa tccttctttg
    35521 ttttttattt ttatatttgc tcctgctaga atcaagtctt caactatttc acttaacgca
    35581 gattgaattg cgagcaaaag ggcagtattt ccttctttat cttttatttc taggtttgcg
    35641 ttagaatgaa tgagtagttt tgctatatca aattgattgg ttttaatcac atgctgaagg
    35701 ggcgtgtctc ctccagcatt ttttatatct atatattttg tggatttaag taaaagttta
    35761 atttgattca tatcctctag ttcaatcgct ttttcaagtg ggtttgcaaa ggtttggaaa
    35821 gaaaaaatta taatggatag gaatagaata tttaacatcg attagcctct ttaatattac
    35881 ctaacaaatt gaatttaaaa attctcagag aatctgtata aagcttcaga gtattcaaca
    35941 acgaagtttt gttttttgaa ttaattctca tttttatttt taaatcctag acagaaacaa
    36001 atcctttaag ccattaacta gaattggcat gcgagtatat aacgttttaa aatttaaatc
    36061 attacaattt taattgtaac tgggaattct ctttttgcga ttgagaaaat agatttggtt
    36121 gagacttgtg atataaataa aaattgcaat gctcgaaact ggtatatcac aaatcgtttt
    36181 gaatcatctt ttttaacgac agagagtata gagataaatg aaacatggaa gaaagtagag
    36241 aagtttccaa ttatcgggaa taaatatttt ccttcgaatt aatctttaga aacgtataca
    36301 attcatacct attttgatat ttcagctgaa tggctcaaaa gcaataagca gttaggaata
    36361 tatttttcta gaattggaca agtctataaa atctatatta atggagtaga gatagcgtct
    36421 gacggactag tgcaaggaga taaaattcta tatgatcgtt tttccaagaa tcagacatgg
    36481 gaagttcatt ctaaaatatt aaaaccacag aacaatctac ttgttataaa aattcaagga
    36541 gatccaagga tttatatgac aggattatac aattctaatc actattattt tggtttttac
    36601 aaagaaattt tagacgtgaa tagagatatt actggaattg ttttatgttt catgtatttg
    36661 atgataggaa gttatcatct tcttttatat ttcaaacgaa gaaaagaagt gtataatctt
    36721 ttttttggtc ttgattctct tggagtattt ggctatttta tttttagatc tagcattcct
    36781 tatgatttgg ggatggattc ttttacagca cttcgtttag aattgacgat actatttact
    36841 ttagttcctt tctttttatt ttttatagag actttgtttt tctcaaaaat cagtaaaatc
    36901 aatctttctt atgcaatagt ctctctaatt cttatactag gtgttatttt ttcaaaatcc
    36961 tatattgtga gtaattatat tttaaagata tggcaactca gtgctttaat cagtgtaatt
    37021 tttattatct atgtttttat taaagcaatt cgaaagggaa acaaagattc taaaagactt
    37081 ttcataggaa ctttgatatt tttactagct gccattattg aattagtgga ttctctattt
    37141 ctaaaaatgt caatagggtt tacgcattat gctttctttg cgtttaacgt tggtattgca
    37201 acggttcttg caaatcgatt cctcacactc tatcagacaa ttgaaggatt aaatgaaaat
    37261 ctagaaaata agattttagt aatagaggat ttgaatcaaa acctagaaaa gaaagtagaa
    37321 gatagaacaa aagaattgca acaaacttta aacgaggttc gtatcttaaa agagaaacaa
    37381 gatggagatt attttttaac tacactcatt gtagaacctc tcatttcaaa tttggtccaa
    37441 aatccaaagg taaaagttta atttatcatt gagcagaaaa agactttttc ttttaagaat
    37501 aaagaacatg aaattggagg agacatttgt attgcagata caattactct tatggataga
    37561 gaatatactg tttttattaa tgcagatgct atgggtaaat caattcaagg tgctgggggt
    37621 gcgatagttc tcggagtcgt ttttaaatct ctaatttcgc gtgtgcaaat ggtaaaagga
    37681 aatcaaaata tctatcctga gagatggtta aagaattctt acatagaatt gcaaagtatt
    37741 tttgaatctt ttgaaggctc tatgcttatc tctgctgtat ttgggcttat agataatgag
    37801 actggttgta tgtattatat caatgcagaa catccttgga tttcaatcta tagagatgaa
    37861 aaaactattt ttattgagaa tgctttgtct ctgcataaaa ttggaaccca tcttatgagt
    37921 cagagtttca gtgtaaaagt gtatcaatta gaagatggcg attctatttt tattggttca
    37981 gacggaaaag atgatgttct tttgagttat gattcagagg gatacagagt tatcaatgaa
    38041 gaagaatcta gatttttaca aaaggtggat gatgcgaaag gagacctaga acttttatat
    38101 gataaattag tagaaggtgt ggaactatcg gatgatttta gtgttttaaa aataacatat
    38161 caaaatacaa atcttactga atatcgaagg attgattcta gtataaaaac tttattagaa
    38221 aaagcatctg cattcgaaga aaaaaatgat tttacaaatg ccaaaaaaga atacgacaat
    38281 gcgtatgaaa tacattcaaa aaacttagcg attttaaaaa agagagcttc tctttatttt
    38341 aaaatgaaag actactcaaa agctaattta ctttttcaag aatgcgcaga attggaaccg
    38401 gcaaattcca ttttactttt caatattcaa gtgaccgcaa aggctgctgg taatttagat
    38461 atggcgatta actttgggga aagaattcat attcgtgatc catattggat cgaaaattta
    38521 tatgaattat ctgactctta ttacaaaagt ggaaatatag atcgtgcaat taaaatttta
    38581 caatttgcag aagcacttag ttctaaagac ccaaaggtaa aacgtttata tgatcgattg
    38641 catgcatcaa attaaaatat cctctgtttt tttagagagc tgtttttcag aaatcaattt
    38701 ttaaaacata ggatacccta aactcaatgg aatggacgca atcaatctat ttatctgttt
    38761 attcgcttgg gcgttttccg gtttttttat ttagtatcat tatcgctttc tatttacttt
    38821 ttcatccggc tcgttcgatt caaagtatta ccgctggggt ttattttcta tttgcttctc
    38881 tctttcactt tggattttta tttgctcact cggtgaatca tccttttgga gcttatggat
    38941 attatttaac tgctctttct ccttttggaa ttgccatctt aattcaattt gcctatattt
    39001 tcccaagaga ctatctgatt aaagaaagaa aaattgtttt gtggataaca gctattatct
    39061 ctttaatcgc aattctagat tattcttatt ttgcttacac aagtggagta ggtgcattta
    39121 cagcaggcta tggaagttat tatatttcaa aactcgttcc ttattcaagc gtagcatttt
    39181 atctttggtc tatttttatt ttcattcgaa aaacgattta ccactctagg acatttcatg
    39241 cagaaaataa aaaaagtttt tcttatctat tttcaccagg cggaaaggaa gcaattacga
    39301 gtagaaattt tgctcttctt aatttactcg aattaatcaa tacttctatt ttaacttctg
    39361 ggcttctgtt tcgaatgttt ccctatattc attttcttac gctcatgaat atttcttttt
    39421 ttgcgatcaa tacattctat gtaattttat ttttgcgata tttaagtgga tctactccca
    39481 tttcttttaa gattatcaat tttggattgt tgtcctctct catcctcacg atttttgcag
    39541 gaaatattca aacgatttat gcagaaaaaa atttccattc gatacgttta aaagaaatag
    39601 aaacaattct attgttaaaa gatttgaatg aactcccgag taacgatgct ttagaaaatg
    39661 tagaaatgat tttacattta agagagaatg gttccgaaat tatctatcaa aaatcagatc
    39721 taaacattcc agataaatta aatctttggg aaaaaacgcc cggactcatc gaatacaatc
    39781 aaaaaccaga atatccaaat ttttcggact tagaaaaaaa agaaaaagaa gaattctatc
    39841 tcagacttga ttcgaaaaac attcagttct attccgttca ttacaaggga gagtggtatg
    39901 gaattggttt taatttttta caatatcgaa aatggattca tccacaaata ctcagtttgt
    39961 tttctcttta tttcctttgt cttgtgatta attttttact ccttccctta aatcttgcaa
    40021 agaatttcag tcagcctttg gaggaaattc tctctgggct ttatgaaaaa acagaaatgc
    40081 tccttctacc ttctagaaag aatgttaaaa atgaattaga aattttaaat gaaaccctac
    40141 tgcatatcaa agaagacatt gagcaaaaag aaattttgca aaaagaatac aacgacttaa
    40201 aagaaaagct agatgagaca gaggatccaa agacaaaaga aggaccctta aaagacaata
    40261 cgattcaaaa agtaaaaaca gtagaagagt atttaaaaga aaattataat tatgaattat
    40321 ctagagaagg acttgcctct atggtcaatt tgagcccagg gagattagga aaatatttca
    40381 aattgtccac tggactaaaa atcagtgagt atatcaatca gttgagagtg gacagagcca
    40441 aatcgctgtt aattgaaacc aataaaacag tcatcgaaat tgcttttgaa gtaggttttg
    40501 agtcgcttag aaccttcaat cgagtctttt tctctgaaat gcagatgaat ccgggacaat
    40561 ttagacaaaa aaaccagaca atttgaaaca tgactcagac tgaccagact ttgttttctt
    40621 tttgtgcgac aatgatctca taaaacaaaa cgaggtaatc gccatgaaag caaaaaatca
    40681 atcagctcta aaatcattct taattttaac agttctatct gttttagtaa attgtggtcc
    40741 tgagttaaaa aacgaaaaga aaaacaaaac tttaaacaac ttagctattt tagcagatgc
    40801 aaaaccagca agatctgaat cgaacttcaa tgaagcaaat gcactcaata cgagatctct
    40861 actcggtcct gatcaaatca tacctacttc ctctctcagt caggattctt cttctgaatt
    40921 gcttggaaat atttatacga atataaaaaa tggagattca aatgtagaag tgaatcttcc
    40981 tttggaagta acgcttttga atggactgat tgcaaataac actggaatca agaatagcta
    41041 ctttctcaca ctagatggta gtgttcttta ttctagagtg gttgtagagg gaaataaaat
    41101 cactctcatt ccacagattc gtttgcagcc aaatcgaaaa tacatagcga tagtcggtgg
    41161 aattcaaaat gcaaagggtg aatctctcgg aaatctaaaa atcaatttta aaagtagaga
    41221 tctggactac ggactctact ggtatggaaa attcggagtt tgtgaaaaat attttccaaa
    41281 tctaaaaaat gctttttaca atcccgctag tgcaactgta attttctctc atggactgca
    41341 aacaaattca gtgagtaacg atgatcctta tggaagaaaa ggatttagct atcaaatgta
    41401 ttactatcta gaagataatt tcgctggagc aaaagaatta aatggtctta gaaaatttac
    41461 aaaccacaat tggattgata aaggttggaa tacagggatt gtattttgga atcagtttgc
    41521 ggatgacgca cttcatgcag cagaagctaa gatttggaat ttagtagaag gaccaaatgg
    41581 aagtagttat aaaacaatgg atgaagcagg aactgtttcg agcaatatat gggacagaaa
    41641 tttacagttc aaaggacaaa caatacaagt ccaatcagtc ggagaacttt taagtcttta
    41701 tgtaattgat gcattaaaac aaaatacttc cggtaatatt cgcatgactg gacattcgct
    41761 tggaaaccaa atgattacaa acatatcagg aagagtaaat gcaagtggaa ttaaaatcaa
    41821 acgaattgca ctccttgatc ctgcttggac ggacggtgca aaatcttatt taccaaaggt
    41881 aactgaaaaa gaaatgcaag ttgcgttaaa tcacaacggt gcaaaggcat tagaacttgg
    41941 aatcgtagga agaaattttt atgtaactga atatgcaaga gtgattttat ttacaattct
    42001 aaatgatcag tggaaaaatg gaatcgtaat tgaaagatac aattctacaa ttctggacct
    42061 ttatctacaa ccttttatga atccaaatca accactcaac caacaaattg ctgtgatgga
    42121 tatgaagcct tggtattact ctgcaactca actaaaagaa aaacacaatt cagttcgtca
    42181 gcattatttc tggtctatgg aatcttcttc tccgattgaa tgtgtgatcc aatttttcaa
    42241 tagaattaaa acgggtgaca ttgcactcag cgccttcact cctgactcta aagttagaga
    42301 gatgatgtta ggtggtaagt attggtctca agtagaagga cgatatactg cagatccttc
    42361 cgatgattgg tatgaagtaa aaaagaaata gtgcggtaat tcatagtagc actttgtgca
    42421 ctatgagtca tttggcaggg aagcctgctt acggtttggg gagaagtcgt ccccaaacct
    42481 cttttataat ttattagcac tctctaatat tcttttatca acttgaaatt ctgaaattaa
    42541 aataaagatg gttttctctt tttaacgcaa tctcaagtaa aatacttact tggaaaaaaa
    42601 agcatattct atctgtatgt aaaaaagatt tgttagccaa ggaaatagaa tgagcaaaag
    42661 cacagaagaa tggggaagta gaattgggat aattttggtg gttgcaagtg gagcaatcgg
    42721 gctaggaaac tttttacggt ttccagggca ggctgccaag tttggcggag gggcttttat
    42781 gattccttat attattagct ttttaatttt aggtattcct gtttgtcttt ccgaatggat
    42841 catgggcaga atggctggaa agcatgggca tagtgctcct aacatcttta aaaattatct
    42901 cactggtatt cctcttcaag tcacaagtgc aattgctctt atcattccta ttatgattta
    42961 tacctattat gtttttattg aagcttggtg tcttgcttat gctgtggatt ttttaacagg
    43021 ttcgatcaat ttacatcctt ctcatacgtt aggcacatca aaaactgata cactcataca
    43081 aacctcaact gaacatttca atcttttgac aggttcactt tccaatggag cttcttttca
    43141 aagtagtatt gtatactatg tattagcttg ctatgttatg aatttttatt tagtttatcg
    43201 tgggatagca aaaggtcttg aagcttttgc aaaagtagct gtgccagttt tactcatctg
    43261 cgcttttatt attttaacaa aagttttatc tctcgataat atttctgcag gactgggtag
    43321 aatgtggaat ccagattggt ctgcactact tcgtggagaa gtatggattg cggctgcagg
    43381 tcaaattttc ttttctttat cagttggctt tggaattgtt ttaactctgg cgagttactt
    43441 aaaagacaaa gatgatgtgg ttttatcagg attatccgct gcttctctca atgagtttgt
    43501 agaggttgta tttggagggc ttatcaccat tccggttggg tttttatttt taggtgcaag
    43561 tgtggttgcc tttggaacat ttggaatggg ttttgttgcg ttacccgcaa tcttttctca
    43621 aatgcaggga gggcaatttt ttggggcaat ttggtttttt attttgttta ttgccgctct
    43681 tacatcaagt gtgactatgg ttcagcccgg aattactttt ttagaagaag gttttcattt
    43741 cacaagaaga caatctgttc ctattttatt cgtttttact ctaatcatca cacttcccat
    43801 tgtttatttt aataaaaact tggctgcact cgaccataca gatttttgga ttggaacttt
    43861 tatgatctat attctggcta cgattcaaat cttaatttat ggatggaaga taggcacgaa
    43921 agaagcgaga gaagatggac taaaaggtgc gttaattggt ttaccacccg gatttgattt
    43981 tattattaaa tatattactc caactttttt aattattatt tttacagcct ttgttttgag
    44041 cgaggatggt ttaatgaatt catttaataa aatgagtgag tcctatatgc ttaccaaagt
    44101 gtctgctacc ttttctgctg aagatgcaat caatcaggca agagttgctc gaggagtttt
    44161 tgtggggata attagtattt tcctcttttt tgtattttta gtgaacttaa gtcttagaag
    44221 gaaaggagaa ttaaaatgaa tatacaaggt gcaattataa tgattttatc tattacactt
    44281 gtaactggtc ttgccggatt ttgtgtgatt cgaattttaa aaggcgatgg aaaggtaaat
    44341 aaaagttagt cgcttcctca ttctttccag agtctacgaa tttcttctaa catttcatat
    44401 ggatcataga tcacaattct tgtattgtct aaaacatcta agaagccgac ttcatttata
    44461 aaacggggaa ctatatgttc atgaatatga gggatagatc cgccgctatt tttaccaagg
    44521 ttatacccaa tattaaatcc ctgtgctctc caggcttttg aaatggtttg aattgctttt
    44581 actctcatac ggtgcatgtc taatacttcc tcatcactta aatctgttat aactagcgta
    44641 tgacgctttg gaaagataat gatatgccct ggattatacg gatataaatt tacagacaca
    44701 atcgaatgag tagattcatg cacaacaaga ttgggaactt ctggattttt ttctgtaaca
    44761 ccacataaaa tacattctac ctgcggtctt tctccccgtg cataacttaa tttttttata
    44821 gagaacaaat ttttacgaat tggtttcaat tcttctgttt ggtcatcgat ttccatacta
    44881 gtttgatttt aaaaaacgca acaatcttgg caagaatttt agatttgcaa atcgagttaa
    44941 ttttttagat tggaaagaga aggtttcatt tgaatttaat ctatgttgtt gttcttgcaa
    45001 tgatcatctg gggagtcagt tgggcttccg caaagatgat tacgaattat actagtctag
    45061 aagtaatttt attttggaga ttacttgtta catttctttc tctaatacca atcatgtgga
    45121 ttacgaaaga tagttttcga atccccaaaa aaggaattcc ttatgcgatt gcaggaggaa
    45181 ttgtaatgct catttacaat gcgctgtttt ttttagggct aagagaaggt cttgctggag
    45241 caggtggagt gtttgtgaca acactcaatc cgattataaa tttttttcta gttgctcttt
    45301 tttacaaata tagattgcat ctcttagaat acattggtct tagtcttgga tttatcggtg
    45361 gacttattat tttaaaagtt tggaatttat ctttagatca agttttaaag ggaggcaacg
    45421 catactttat attagcctct tttacttggg caattctcac aatcattacg gctacttcta
    45481 agagctatgt ttctccaatt acttatagtc tttatttata tggaattgga actatcgtct
    45541 ctttttttat ggctcttcca aaagactgga tgggagcttt ggcatttgat tctaatttct
    45601 ggtggaatat tttttactta ggttccattt caacttcttt tggaactacg gtctatttca
    45661 tcgcgtcctc tcgtttaggt tcggcaaaag ctagctcttt tatttttttg gttccagcga
    45721 gtgcaatgct tagtagttgg ggattgttag gtgaaactcc tcagtggact actatcattg
    45781 gaggacttct tgcgatatcc gctgtgtatt tattaaatag aaagaagtaa tttccaccta
    45841 tgaaccgttc ggctccgctc atcgcaccgt tggacacaaa taaaagatag aatttgattt
    45901 ttgatttaga tgagtgtcta taatgtattt tgattttagt ttctaatttt aaatcaaaaa
    45961 aagaaaagat tttaaattgc gacttcaaag aatggataca ttgcatttac attaagcaga
    46021 agtttgcttg ttgtaagaaa aaaatattaa attaaatcag tgcgatacag tgtggattcg
    46081 gtaaaaacta aaaccaagct gttgatacgc ggatcttttg aaggataatt atggactgta
    46141 aaatttgcga tatacatcat actaaatcaa ttccaatgct tgtgggggaa acctttcatt
    46201 gggtcattcg ccattctgaa tttactaaaa atgctcctgg atatttatac attgaaccaa
    46261 agcgacatgt ggaatcttat aatgatttaa ttcatggaga attcatgcaa ttaggcgaat
    46321 gtatgaaaat ggctacagaa tggatttata gacatttcaa accattaaaa ctctatacga
    46381 tcactgtatc cgaagcggtt cctcatttac attttcattt ggttcctcgt tttgaagatt
    46441 caccgaaagg aattgagttt ttacaagagg cactttctgg aaaattgcaa gcaacgccaa
    46501 aagcacaggc ggaactagaa agaatattga agttgtttga ggagaattag tcgatagttg
    46561 gagaatctta acttagatta atgatggtaa ataaaaagat tacaaccgat tcacaccgat
    46621 ggacactgat aacagagcta tgattgaatg atggaagagg gtttattcat gtagaggcaa
    46681 atattgaata gccgcataga ggatgaagat gagatgaaac gatgttaggg ttaataaata
    46741 cgatagagaa taagctatag ccgctagaga gaaaaaacag gactttttgg agattctttt
    46801 taggtaagaa tcaattcttt atttttgtta tatatttcaa taccaactct atctatatgg
    46861 tcttttagtt cttttgactc gatgctttta taatcaagaa tatcaaaaaa ataaatcatc
    46921 gggctttctt catttaaata gtaatttata gaagttattg tatcaaaagt tatcttttct
    46981 ccaaataaag cgatatctat atcagaaccc tgtttaaagt ttcctttagc tcttgaacca
    47041 aacagaacag ctttttctat ctctcgctta ttgttaaaat atttaacaat aagttctaaa
    47101 tcagtttttg ataagccata tttcataata ttaatttatt caattttaga aaaatattca
    47161 acaagctttt taatcatttg ataatatttt gaggtgatta attcttttgt ttgtaaagta
    47221 gtttgttcat tgtatgtatg tgacattaaa tttctttttt ccaacgcatc caaccaaatt
    47281 acaccatctt ctataatatt gacttgatat gcttttttta tgacttctct tggcgtttta
    47341 acatcatatc cttcgtgttc taaatagtct tttaaagtct tccaagaaag ttcaaaagta
    47401 tattcaaaac attgtataag cccttggact tcaaacttat tcaattcaga aacttccata
    47461 aattcggtaa gttgatcgta tgctttttta aaattactat atctttgttt ccatcttata
    47521 tcttggttca ttttattacc cttgtaatat taaataatta ctatgtataa ttgaaatggc
    47581 ttcatcaagc catttatttt gtaaaacttc atcataagct gctggaagtg taattgaata
    47641 taattcatct ttactcattt ctaaaagttt ttcaatatcc tctttagaat aatcattatt
    47701 atctaaagaa ctttttaata aatctatcaa gtattcaaaa gatgaatttc ttaaatcagc
    47761 aaattctccc attaaaaact cctaatatcc acttaattcc ttaattattt atatgtatag
    47821 aaaataatta ttcgactata ccactattta tatcctcaat catttcttgt taatttttaa
    47881 ctatcatcct ggactcaata gtagttggga tattaaaaat atttcaaaat aaaaattatc
    47941 ttttcttata aacggctaat tttaaatatc cgcaaatgta acgaaaaggg tgcagaaact
    48001 aaccgatagt cgctagaagg aaaaaccgac tcttttcact tgattgaagt tcgataaagt
    48061 aaaattccca atatctgatt cttcgattgt atccagggtt tttctgttca tcaaatcacg
    48121 tggactgagt gagattattc tctgtgcttg atttttaaaa gcctgttcta gaatgttacg
    48181 cacggttctc gcattggcaa attcactgtc tttctctttt cgttctagaa gttgtgaaat
    48241 tagtttatta tgagcagcct cacttaattc atagccgtca tttttacaaa tatcataaca
    48301 aataccactt aactcttcgt ctgtaaaatt aggaaagact aacgttttac taaaccgacc
    48361 gagaagtcct ggacttgaag ccattagttt atccatgggc tcttggtaac ccgcaaaaat
    48421 taccacaagt tcgtcgcggt aatcttccat gtatttgagt agggtattga ctgcctctct
    48481 tgcatacata tcttccgagc ttgcctcagt aaaaagagaa taggcttcat ctacaaaaag
    48541 aattcctcca cgagcatctt ctatacaatc ttttgtcttg attgcagttt gaccaagata
    48601 tccacctatg agccccgaac gatctacttc cactagatgt ccacttggca aaactcccag
    48661 ctcccgataa atatctccga tcaatcgcgc aactgtcgtt ttacctgtgc cgggatttcc
    48721 ggtaaatatc aaatgcaact gcaaaccgcg agaagaggaa gcaccttttc ggagtttacg
    48781 aatccggata taatctgcca tagcctgaac ttcttttttt atttcttcca tgccgcgtaa
    48841 cgcatgtaat ttataaattg ccgatttcgg attttgtctt ggatcaacag gaggcaataa
    48901 agcaatctca gaacctccct catcatttgg atcggctgta atatcgctat aaataaattt
    48961 ctgtaagttc tcttttgtaa tcttccctgg ctctaggttg tttagtcgaa tgcaatgttg
    49021 cgctactgct ctttcgaaaa cgtttcttac ttctcttgca tttccaaaac cctttcctcg
    49081 ttttttaatt agaatttgtt caatggcaaa ggttctgtct tctttgctaa tattcatttc
    49141 gtgtttggag caaagattgt cgaataagat tccgatttgt tcatctgaga aatcttcaaa
    49201 tggaacaata ttaggaattc tactttttaa gccgggattc agatctagaa aacttctcat
    49261 catatcagtg tagcctgcaa gaattatcac taattcatct cgataatctt ccatatattt
    49321 tattaacgta ttgatacttt cttgaccaag cgtatcgttt ttatctactt ttaaggaata
    49381 tgcctcatca ataaatagaa tacctccacg agctttttct ataattgcag aagtgcgtgg
    49441 agctgactga ccaatgtatt ctcccacaag gtctactctt gagacttcaa ctagatgagc
    49501 ctttggtagt attccaattt ttttgatttc atccgcatag tatctggcga atgtcgtttt
    49561 tccagttcct gagttgccag aaaaaactgc gtggaggttt actttgaaaa ctggcaaaga
    49621 gttttttttc ctctcctcgt tcacttttac catagcaaga acttgtttca gagcagtctt
    49681 cgccatctct tgacctatca gatcggactc agtgactttt aagctatcgt ctaactttac
    49741 tcctcttgtc agcggagcaa gaagggatac gatagagcta tggttattct tttgtgcata
    49801 gtcgtatgct gtgaatcctg tcttgtcttt aaaattggga tctgctttgg cagcaaggag
    49861 actatcgaca atatccttcc ttcccatttc agcagcaatc atcaaagaag tttttttatg
    49921 ttcagctgaa tatccatcta ccacagctcc ccaactgaga agagtgttta ccatatcgag
    49981 tcttcccgtt ttgcaagcct gtccgagagt agtaacaggt ccggaagttg taaagtttgg
    50041 atttgatcca tgtttgagaa gaagttcgag tatatcatgt gaacttcttg cagctgcata
    50101 atttatggga gatgcaatat cgggaagttg gaaattggga gtaatccctg tttcaagaat
    50161 tgctttaaca tctgcaagat taccggtatc tacagcagtg attaattttt ttcgtagttt
    50221 ttctactgtt tcattaaatg tatctggcag atccagattg ttgttatctc ccatatttcc
    50281 cccaagtcct atttaggaat gaatttttat tctactgaat agttttgcaa tcaaatttaa
    50341 ttagttttat tcaattcgaa accacttcta taaaacatcc taatttgttt gtaaatgcgt
    50401 atgagtaaaa tcagactttt tcttagttgt ttcaataaat ggaatggaaa ttctattttt
    50461 gggaacaagt agaatgcaat cggaaatatg cgatagtcac tattaataaa ttagaatatt
    50521 ctaaattact aatttatttc tggcttggtt tctactaaat tatctctata aaatttatct
    50581 acaattgaaa tttacaagag cataaatgga ttttcttatt atagaaaatt tcgtatcaga
    50641 cttagtatct aaattggatc agaccaaatt tcattcggtt tgaatctgtt gtcttcgaaa
    50701 tctcgaaaga aacgaaaact gctgattttc catatatccc aattccagat ttgtagcctg
    50761 aaaatagact tattatgaaa ataactcaat tttttatgga atggagcata acgtaattag
    50821 aatgggtata ctcaattcca cttgcaagtt tgtcagaata tcatttctat tctggagaat
    50881 taatggaatc ctccaaataa aattttaaat gggattatag aaaaatgaat agagtgagga
    50941 aaagacgaca gtcgctagga ttaaagttta tgattctttg tttatctttg gtattctaca
    51001 atgctgattg caatcatggt tttactaaga ctcgtataga gagatttata aatggtgtgc
    51061 agggtttttc ttttggataa ggacaaaatg gaatcgaaat tagatttgct ccatttatta
    51121 tgttagcata aaagatagtc cagtaacaga actaaagagg aattgaaaac agaaagggat
    51181 gcctatttga tcggttatgc aattgctatc tataaactcg atgagtgtaa gaaagaaact
    51241 aatatttggg gattgtagag actcaaaaga accccacaag ataggaatga ttctatatcc
    51301 attttctttt ggagaaatca atcctttata ttgagtggaa aaaagtattt acgtataaag
    51361 tcatattcga tacaaaagtg gagagttcta gattatacta gatgcttaat ataaaaacga
    51421 taagcgaaat gaatgaattt atagatgagg aggagatttt cttttatgtt taaatttaat
    51481 actaacaatt tacaatggtt ttgtaaacct aagaatttta ctgttacaga agaaagaatc
    51541 gagatcataa ctgaaccaaa tacggattta tggcaaaaga cttattatgg atttcaaaat
    51601 gacaatgctc atatagttta tgtgacagca aaggaaaaat atttcacttt tactgtaaaa
    51661 acagagtttg agtattcaga gctatttgat caatgtggaa tcgcaatcta tcaaaattct
    51721 aataattggg taaaagcagg gattgaattt cgaaataaaa attcttcctg gctcggaagt
    51781 gttgttacga ataacggata ctctgactgg gctactacag aattggaaac aaactacaaa
    51841 tctatgtggt atcgaatcag taggagagaa aatgattttt gtatagagaa ttcagttgat
    51901 ggaaaaatat ttaaacaact aagaattttt catttgtttg atgcgaccga ggatattcgt
    51961 attggtttgc tcgcttgctc cccaaagaat aactcattta aagcaatttt tacaaatatt
    52021 gaagtaactg aatgtttatg ggaagagcat aaataaacaa attgtaaagg atattgatag
    52081 taaatctaga aatgtgcgag tattcatttt acgcaaatag gcagaagtaa agaagtcact
    52141 agcgcttgat agctaacata gaaatatgag agtgaaaaga atttaaatta ggcgatagcc
    52201 gctagagaga tataatctgt ctcagatttt tctaatgata gaactataaa tatccttgac
    52261 taaaaattcg gcatcagata gataaattta taaattactt ttggaaaaat atgtttctct
    52321 tctttaaaaa tatgatccgc aaaattaacc caatgagttt tttctttgga attctttttg
    52381 gttttggttt acagattttt acattttcat ttatctgggc ttttgcaaaa attgaaatta
    52441 ataaaataaa ttcgccagaa gtaattattc cctctgtgat ggcagcattg ttggctgctg
    52501 tattagagga gtttattttt agacgtttca tctatcgttc tgttctatct cggtatggat
    52561 ggaaaattgc cgtttccgcc agtgctttag tctttggaat cctccataca tttaacgatc
    52621 atgtttcctt cttttccatt ttgagtatca tggttcaggc tggtatttta cttcctgttc
    52681 tatatacata tacaaatagc ttaccatttg tgattggctt acattttgct tggaatctta
    52741 tacaaatggc aattttcgga acacctacat ctggaactaa aagctttgga agtttatttg
    52801 aaattaagct tttgggaaat gaactcatca ctggcggaaa ttttggagtg gaagcttcta
    52861 ttcaagcgag ctttatttgt ctggttttat tcctagggat catcaatagt aaaaagttcc
    52921 gtgaacatgc aaagcatact ggaaaatggt ctctaaagta ataatgtttt tgattctaaa
    52981 ttagtcttgg caaattgtaa aaaggaaaac cttcacggat tactttattt aattttataa
    53041 gtttttccta aatcagaaat ggatactttg attaattcct ttaagatgct taagtctata
    53101 tcgtctgtct ttttaatata aatacaggag ccgccgctta ctttgtgttt gcctaatttt
    53161 tttaagagag ctggatattt ttttacacct gacatgatgt agatcgttaa gtttgttttt
    53221 cgagaagaaa agcctgtcgc catccaatct ccttcttgac tgcttcgttc cgatttataa
    53281 tggtattctc cgaatccaat ggttccattt gaccaaagtt tgggtttctt gcgagtaacc
    53341 gctttgaaga tttttaaaag tgtcagagca tcttttcttt ttgcttcatc ttctatttcg
    53401 ttgataaatt ctgatacatc ttttgtgata gattttgttt ttatttcggc catattattt
    53461 cctctctatg acttttttct atttactata tctagaatgc aagggaaaag ttgatgagta
    53521 aaccaaaaaa gagaattata tattcgtaac tctacttacg catgagcgag taagttgaga
    53581 gagggagcct ttcggccaat gctatcaact taagagaaat aaaaaagaat taaccacaaa
    53641 gagcacagag aacacgaaga aaagagaagg tttattattt attcacttag tgagtcttcg
    53701 tgcattcccc ccatcgcttg acgctcacaa taagccaagc tatgtggttg aaaatcctta
    53761 agctgacagc attacctgtc ggcaacactt cgactagctc tcagtgcatc gcttgagcta
    53821 accacgcaaa cctgcttatt tattaatcca tttgagaaat gtaatctaat attctttttt
    53881 attgatttaa taaattttta tcttttgcgt aatatcagtt agtcaatgat ttatacaaaa
    53941 aagataagtt gagaagtaga ataaagaaat aatttatctt ctaaatttgt aatttatttt
    54001 ttaaaaatgg attcgaatct atagcgcttt gaaataattg tgtattatgc cgattgaaaa
    54061 aatacaaact caactaggga aactttccaa tccaactaaa gcaaaatttc tccaaggata
    54121 tttcaaaaca ggaaaaggag aatatgggga aggggatatt tttatcggaa ttacagttcc
    54181 aaatcttaga aaggttgcaa aagagtttga atttatttcg attgaggagc taacaacatt
    54241 aatccgctca aagattcacg aagaaagact gcttgccctt cttatattgg gtagaaaatt
    54301 tcacaataag aaagcagatg aaaaattaaa aaaagaaatt tatgaatttt atattaaaca
    54361 tttacaatat gtaaataatt gggacttagt ggatgaagct tctagaacga tcttaggctc
    54421 ttatctttta gatagggata gaagcatttt atataagctt gtaaaatcta aaaatctttg
    54481 ggagagaaga aattccatca ttgctacttt tgtattttta caaaatggag aatcggaaga
    54541 tacaattgca atttctaaag agcttttaga ggacaaagaa gatttaatgc acaaagctgt
    54601 tggctggatg cttagggaaa tgggaaagag agtagatgtt aagcttttag aagaattttt
    54661 aaatacatat aaaagtaaaa tgccacgcac aatgttacga tatgcgatcg agagacttcc
    54721 tgagaaaaag agaaagatgt atttaagtaa ataatgtgaa ttggatataa cagaaaattt
    54781 aaaagattac caccgataaa caccgaggac accgataaaa gatacgataa ttcaaaatta
    54841 tccatcttgt cttcatcgtt cagttatcgg tggtgaaaaa atcttacatc gaattcactg
    54901 aaaataagta atttttattc ccgatcccga ctattccata aaattaaaaa gattagacca
    54961 agcggaatcg taatggggga gagtaaaata tctcctgcga gagcgaaagg gagtaagatg
    55021 gtccaaagaa taaattttcc tacagctgat ttttttaaga tttctttgta ttcccattct
    55081 attattaaaa ttttatgagt tggattttct ttgtatagaa aggcaatgta ttgttcatta
    55141 ctagtattta tcttgatctc tagcattgtg tttatttttt tttgagagac tgtattttct
    55201 tggttgtcta aaaaattaga aatccattca ttcttttctt ttaatacttc tattgatgta
    55261 tttatatcaa cttcctttga attgtgaaca ataagatagg attgattttt taaaataaaa
    55321 acaagatcat tcggatagtt ggtattcagt gcaatttgct caatggaata aaaatttttt
    55381 tttagttttt gggttaagga atttgatttt aatttataaa aagcttgctc atcatttgtt
    55441 tctatttcca gacattcttt tatctctgga ttctcataat atatttcttt gggttttgtt
    55501 tgaatacaaa agttttttcg ggtttcatct gaaaagttaa tttgaatttg atctctttga
    55561 ttccattgtg cacttacatt gggaactatc gtgaaatctg tgggcgaaaa aattcctttg
    55621 ttattgatat acttagttcc agttttttcc tcatagtttc tctttctaat ttgattcatt
    55681 ttatcataga cactgattgt aaaacaattc attgcgagca ttgtatttac taagagaaac
    55741 gaagtaaatg cgatgtattc tttttttaac atttacagaa tcgatgccaa tggcactctt
    55801 aaatgatcat cgtgaaatac ggaatatagg agataaagac gataaactat aatcgaagaa
    55861 atgtatattc gtaacttgat tcacattttt tcattgacta tcgttttttt atcaatgctc
    55921 atcttttaaa aagatataag taaggataca tcttagaatg tataattttc tggaaataat
    55981 cttacagaac gttctacttc tctatccaaa agtttctgaa tctcatctcg gagtcttttt
    56041 gttagatttt ctacagttcc ttttttgtct tttttatatt caggataata ttctgatatt
    56101 ttaaaaggag gtgcaaaatg aacaaaggct ttttgaggac gttggtaggt atagccaatg
    56161 agttcacttt cgaatcgata aatccactca tagattctct ctgcagatgg aaagcttgta
    56221 aggtaatctg ttttaatcga aataaagtca taagctcttt ggcaatattt tcttccccat
    56281 ttagctttat ctagcgaggg caattctccc ttaggatcag gaactcctac aaataccatc
    56341 tcaaaagtgg aaagaatttt tctgagttta tcgattgcat gatcctcttt gttgtatttt
    56401 tttaagtcta ctgtttcggc taccgtatct aaaattctat gtctaagtcg tccaattcga
    56461 taatcgaatg tttggttttc atccggcacg attgcaaatt gtttttctgc tctttcaact
    56521 aaacgttttc caatagatag aagacggtgc acaatatctt ttccttgttt ggaaattcct
    56581 aatcgttttt ccatcacttc tagtgattta tctacattat ttctaatttc ttctatagaa
    56641 ccggacaacc tgtatttgat aaaagcgggt aaaacagtaa tgtctgcttt tgggtcttgc
    56701 ttgattgcat cttcataacc ccaaaaacca agttgagaaa ctcctggctg aaaaggtaat
    56761 aaattatcat tttctgctcc agtcggttct ccttctggga ataacaccaa tttgccgcct
    56821 tctttagcaa gaatggctct tgtagtcttt aaagattctc gatcggatgc accagcaata
    56881 acagaaaatg caccaatgtt ttgaatcata tttccgacta aaccatttcc ccaatcaaat
    56941 acttcacggg aagccatgta gttgaatcta gaatacatat ggtttccaat gatataggaa
    57001 ataggaggct cttttgtaga ggggtgattg gagataaaaa taagtctttc ctttttgtag
    57061 gtttttaact tttctttgtc ttcttctgtg atgacaattt catcaacatt gttgacgcct
    57121 ttcattagaa cctttaaagc ggcgtccatg aaccacataa aaggataatt gtgtattggt
    57181 ggaataaatg aatcagttga catattgcaa ggatgcagaa tttatcaaat agaacaagaa
    57241 atttttataa tgaattcagt gaatctctga attagtaggc aatactagag agtttttcac
    57301 tggaacgata aatgaataaa ttaaacagaa ggttagtatg gcattaatag aagagtttga
    57361 gaaacaagga tcatttttat ttcgttggag atcgtatgtt ccttcggtga ttattatttt
    57421 atcatttttc tttatacccg attacaggta tccggatgga gattataata aacatttaat
    57481 ctatgtagct ctttgttttt tagtgagtct ctttggactt tttattcgat gttttacgat
    57541 aggttacgca cctgagaaga catctggaag aaatacaaaa aagcaagtag cagaaacagt
    57601 gaatcaaaca ggaatttatt ctttgattcg tcatccccta tatgttggaa acttttttat
    57661 gtttgctggg attactctac tcacaaaaag tctgtcgttt atcattatat tcttttttat
    57721 atattggttt tattatgaaa gaatcatgtt tacagaagag cagtttttac gtgggaagtt
    57781 tagcacccaa tatctagcct gggcaaataa aactcctgcg ttagttccta aatttactgg
    57841 atacaaaaca cctgatgtta gtttttcttt taaaaatata ttaaagagag aatatccaag
    57901 tctttttgga atcatgctca tgtttgtttt ttatgatgtc atcatagtct atttcaatga
    57961 accagaactt gtggcaaaag gttttcctga tttattaaaa ccaattcaaa tctatacagc
    58021 gatagccggc ataactttct acgtcatcgt tcgtattatt gttaagttta cgaagatact
    58081 gcacgttgag gggcgttagg taaaaagact accacagatg gacatgcgaa ggcgtcggtc
    58141 gcgaatactt ggagcgaagg gtggactgaa caccgatggt ttatctttta tcagagcttg
    58201 tgaatggtta tctcgtttca tcttgcagga tggtaaatta gattttaagc tgaagagatt
    58261 ttaggaagcc agagaagaag ttattacctc ttctcgtttc ctcagtagca taagattgaa
    58321 ttccttgaaa gattttttta aatgaggcgt catgaaaact atgacgactt tacaattaca
    58381 aaaaatgaat tcgatctgga aagagttacc agatgaaaag tttcaattag ccataaattt
    58441 tattttatgg ctaatacagt cagatgaagt tttagctgat atggaagtta agtaaaaaaa
    58501 gccagaaagg aaaaatcagg aattacttgg gagaatctaa gagaaaatgt ataacttaat
    58561 tttcaaaaaa gatgcaagta aatattataa gaaatgtgat caaaaagaaa atctagatta
    58621 aataaagttt ttgatacatt agaaaaaaat ggtattacga atttagattg taaagagttg
    58681 aaagatgagc tttctggttt aaaaagaata agagtcggca acactagaat cataatcaaa
    58741 aaaaaagaat ttattatcct tttaatttcc acaagatgca atgtttataa ataaaatatt
    58801 tctctttcta ctcactgtaa ttgctttagc tttatttggt tttatttatc tttcgcataa
    58861 aatccaggat ggtatgtgtg cgaatgaaat agtctctgaa aagatctctc ttgatttgaa
    58921 atataaagct attgtgttta taagggattg cggtgctaca acgaactatt ctttgcaagt
    58981 atcaattctt cctaaacaag atagcttgga aaattccaca ggaaatattt taattttaga
    59041 tgaatcatat atcgattttg aaaaccacaa aacaactcta gaatatgatt ggaaaaaaga
    59101 taatagtcta tcgattcaga tcgatccaaa gactaaaata tattatcaag tagatcaata
    59161 taaagatata cgaatacaat tgaataaaat agaatgagag aaagaagttt gttagaaaga
    59221 gtttcaattt aaaattctat gaaagaaaac atttatagct ccgaatttgt agaaggttta
    59281 ttcaatcgta tgagcaattc ctatgagagg atgaattatt ttacatcctt tggattttct
    59341 cttaggtgga gacgtcaatt tctaaagttt ttagttccga gtgttgaaag agttaagatc
    59401 attgatttaa tgacaggaat gggagaaaca tggggggcgg ctaagaggcg ttatccgaat
    59461 tcagaattaa cagcactcga tatttcagac ggaatgctca atcgtgctcg aattaaaaat
    59521 cagaaaaaat ttgagggcag tgtaaaaatt gaaaaacaag atattttaaa aaacaaatta
    59581 ccttcgaatt attttgatat tgcaatctgt gcctttggct taaaaacctt tacgcattca
    59641 caaattgaga aaattgcaga agagacctat cgaattctaa aaccaggtgg tcagatttcc
    59701 tttatcgaag tatcgaatcc ttcgaatcga attctttctc ttttatattc tattcatctt
    59761 gggtttgttg ttcccgtgat tggtttttta tttttaggag gacccgaaga atataaaatg
    59821 ctttggcggt atacttctgc tttcggaagt tctaaagagg cattttctag ctttcagaat
    59881 cagggattta tactggaata taaatcttat ttttttgggt gcgcaactgg gttcactggg
    59941 aaaaaaccaa aatctttcag ttaagaaaag ttttaaatat ttacctccga taaagaagag
    60001 gaacggattg actacgttct ttgcatcgca acgatggaag acttgattat tcataaccaa
    60061 tcatcgtttt ttatcgttcc gttatcggtg tcctcggtgg taattattaa gtttagagca
    60121 ttgaccattt gcccgacagc gctgcttcat ttttcacctt tttcaaaaag gcggtgagtg
    60181 agattctaaa atcgaactca ctcaaaatta atgattgtaa aaaagataag gttatatagg
    60241 atttgaagtc aaaattatgg aaataaagaa acgaacatct ggtgatgcaa cgattttgga
    60301 actaacaggc agtctagata tttatacagc cactgatttt aagacatatt tacaagccaa
    60361 tgtaaacaca aaaggggcaa aggtagttgt agatatggag aaattaagct acattgattc
    60421 ttctggaatt ggtatgctta tcaaaagttt aaattatatg aaagagttaa acggaaattt
    60481 acagttggct aatctaaaag aatccttaca aaaaattttc aaggtagcgg gactcactgc
    60541 ctattttcaa tttttaaacg agaaggaatt caaagaaaaa tactctacat gaattcactc
    60601 gatttatctg accatgtcct agaagaggtc ttatcagaaa ttcaaatcca atcgctagat
    60661 tgcacttgga atattacaaa cgaaaaactc atcgatgtaa tgggaatccg caaagaggat
    60721 ttttacagat atctttataa tttaagaaga aatcatactc aaagtatcag tcttcgttcc
    60781 tttagcgaaa aggaagcaga tttactttgc atattactag aacaaatact caaacggaat
    60841 gatattgagg agcaattcat tcgctctggc atttattttc cagaaacagc attagaggaa
    60901 ttaggtcaaa tactagtaga gttgattcaa tcctctctaa aaaaacataa attggataaa
    60961 gagttatttc atttgctcgt gagttctaca aaactttttg atgatgcctt tgattcttat
    61021 tttgctgata aatttgatat ggaagttttg ttcgagagaa gtataagcat ttttattaat
    61081 tccaaaaata ttgataactc ttttggggca gaaatatttt tgaagaagca tttacagagc
    61141 ttacttaaat acaataagat atctttggca aagattacaa aagaatatcg tcagagattt
    61201 tactatgaac ttttcggtaa atttaaacgc gagggaaaaa tggattcaga gacgaagcgg
    61261 cttttagagt tttttgaatt agaagatggt gcaaccttaa aagatttaaa aaagagattt
    61321 aaggaattac tcttgatcta ccatccagat gtaaacaaag aagggcatgc caaaacacag
    61381 gaaatcattg aaaattataa taagcttttt gagttaatac gttagctact ctagaaagct
    61441 ccatccccat ttattaaaat aacggatcat attggagata aatataaaag tgatcttcca
    61501 gtttttatgc gctgctcttt tccaaagatg agttacattt gccctcgcaa aataggtagt
    61561 tttataaccc aaagcttgca cttctctcga aatatcagcg tcttcaaaat ataagaaata
    61621 ccttggatca aaacctctca ctttatctaa aacatttctc ctaaaaagca taaatgcccc
    61681 actcataaac gggacttcat agttttcttc ttggtttaaa tctttcattt cataaaagtc
    61741 taacgttttt ttgaagagag gatgtaaaat ttttggaata aatcttctga taaataaggg
    61801 gagaattgcc ggatgacgtt tatttacaaa ttgctgggaa ccatctaggt ttaaaacctt
    61861 cggtatacta attcctattt ctggattcat atccataaag tgaattaatt tttcgagagc
    61921 atcttctttg ataaaaacat caggatttaa aatcaggaag tattttgatt cttctgcatt
    61981 ttcaattgcg atattatttc cttgtccata tccaacattt cttccgtgat tgaaatgata
    62041 gtagattctt ggatcatcag gaagcggata atttccttta accgcactgt tatcaataat
    62101 ataaattttt actctaagtt ttgtatctag aaaagaatta acacattcca ataaaacatc
    62161 taaagacgta tcatagacta caacacttgc aatacaatca tacacctgat tcatattgtt
    62221 tttttagatc cgaaatgata tttttttgca ttgcttcatt tgttccccct ccaatggaca
    62281 gtagaatcga atctctatga agtctttcca ctggatattc cctacaatag ccatagccgc
    62341 ctagaacttg aattgcattt ctagaaactc tttctgccat ttgtgttgta aataatttgg
    62401 cagatgcggc accgagtgaa tttctagact cagggtgtat gtttgccgct acttggtaaa
    62461 caagtgctct tgctgcagaa tattctgcat aggattctgc aatcattctt tggatttgtc
    62521 caaactctga aagttttttt ccaaatgctt ctctatgtaa aatggaatat tcactcatta
    62581 tatctaaaca tctcttcgca atccctagag attgagcggc taacgtgagt ctttctattt
    62641 ctagatttcg catcatatga gaaatggctc cattttcttc gccgattaaa ttttcttctg
    62701 gaacttccat ttcttcaaaa accaattgag aagtaggaga agatctcatt cccatttttt
    62761 cttctttctt gcctacgctg aatcctttga agcttgattc tactaaaaag gcagttgtct
    62821 ttttgatgtc acgtttgtgc atttttgcat agactaaaaa tatttgtccg atgcttccat
    62881 ttgtaatgta ttgctttgtt ccatttaaaa tatatttgcc attggacttt gttgcgatag
    62941 ttgtcatacc aagaacatct gttcctgcac ctggttctgt catgcccatt cctgcaatcc
    63001 attccccact catgactttt tgaagatagc gtttcttttg actttcatta ccactgtaat
    63061 aaaaattatt tacaaacaag acttcgtggg cgaggtaaga gagcgtgaat ccaggatcat
    63121 atttagacat ttcttcgtga atgattacag cagcgactga atctagacca agtccgccat
    63181 ctttttctgg gactgttacc cctaaaagac ctaattcttt tgctaatttt tgaaatacat
    63241 ctttgttgaa tgtttctttt tcatcatttt ctttagcttg atcatctagt tcttgctttg
    63301 caaatcgact aacgttttct cttaattctg catgagcgtc agacggtgta aataaatcat
    63361 ttttagttgt catggtaaat cctcgtaaat tcctatataa tctaggtttc tatattttcc
    63421 tctatagtcc attccgtaac ctactacaaa atcatcctca atttcaaatc caacaaaacg
    63481 tatattcgat tttactttgt gttttttata ttttacaaga agggagcaaa tttcaattga
    63541 agctggtttt ttctttttta aatatttaag taaaaaattt aaactaatac ctgtatcgat
    63601 aatatcttct acaatcagta aacgttttcc ttttatatcg attccaacat ctcgtttgat
    63661 taagacagtt ccagatgatt cttttccatc tccataagaa gatgcttcta agaaatcaat
    63721 agtagtagga gaactaatct ctctcatcag atcggctgag aaaataaatc cgccttttaa
    63781 gatgcagagt aatacaactt cctctttttt gtagtaatta gaaatttctt ttcctaattc
    63841 tttgattctt ttttttaact tttctcttga gattaatttc ttcatttttt ggcaggagtc
    63901 cagaataatg tcaatttgcc tggatactct gagatattct tccaagagga aataggaaag
    63961 gcgagtccta taatggatga ggtgccaaat ttttgaaaga aagaaaattc tttataagcg
    64021 ttttgtctga gcatcaattg atttgcaagt tcttccaaat ctggattgtg tcctaaaaat
    64081 aaaatcgagt tacaactatc ttccgtagtc gtaatcaaat tgaaaaaaat tccaaaattt
    64141 gcttcgtaga gaagatgagt gatttcaatt tctttaaaga ggtctttgtc tttgtttata
    64201 atttcaagtg tttcgattgt tctcttcgca tccgaaacat aagctttatc tacttgaaaa
    64261 tttctatcct gtaaaaagtt tctgagtgct tttgcatttt ttcttccacg ctctgaaata
    64321 ggtctttcgt gatcgaatct aaattcggct tcccagtcag acttagcgtg gcgaagtaga
    64381 taaatagttt taagggaatc catatttcga ttcagctcat ttgaaatcat ttcggtcaag
    64441 cagaatcttc caattcagtg cacttctctt catcaaaatt gcttattgac tgaaaactag
    64501 aatcgagttc tattgtctta gatatataag aaaaggtaga aatgtatgag cagctataac
    64561 aatgcagaat taaatttaga tggtaaaaaa tttcctttga atctaattac aggaacagat
    64621 ggaaaacaag gcatcgacat aaaggattta tataagaata caggagttat cacagttgat
    64681 ccgggatgtt ttaatactgc gattggagaa agcagtgttt cgagaagaga tccggacaaa
    64741 ggagaattga gttatagagg ttatcaagta ggagacttag caaaaaattc aacctttgtt
    64801 gaaacatcct atctactcat ttatggtgaa ttgccaaata aaaaccaact ccaagattac
    64861 tcaaatcgtc tttctaagca ttctatgatt catgaggaca tgatcaattt atttgacgga
    64921 tttccgggca aagctcatcc acttgcagtt ctttccacaa tggtgatggc actttctagt
    64981 tattatacag atgaatatga agaatcagca gatagaggag tcgactacgt aacacgttta
    65041 ctcagtaaaa ttagaacgat agccgctttt tcttataaga gaatgattgg gcagccattt
    65101 atttatccaa tagataaact tccgttttgc acaaattttc tacatatgtt attttctgtt
    65161 ccaagtgaat catatgaagt tcccaaagac caagataaac ttttaaatca actttggatt
    65221 ctatatggag accatgaaca aaatgttgcg gcaactaccg ttcaattggt tggtagcaca
    65281 aaggctaatt tatttgcatc tatcagtgcc ggtatttctg ccctttgggg atctagagag
    65341 ggaggacaga gtgttgcggc tgttgagcta ctcgaaaata tcttaaactc aggtaaggat
    65401 tataagacct atttatctga ttcaaatgtg agagctgaac tttctcgctc taactttctt
    65461 ggacatgaag cgtataacgt taaaagtcca agagcaatag ttgcaagaga aatttttaga
    65521 gaatatttta agactcatca tagtccttta gtagatttag cttttgaagt agacgaatat
    65581 atttcgaatg aagcctattt tcaagataaa agactatacc caaatcttga attttacagt
    65641 ggaattttat ttcatagcat tgggattcct aagaatatgt ttactctcat gcaagcgatt
    65701 ggaaaacttc ctggttggct tgctcattgg agagaactta gaattcgtag taacttcaaa
    65761 aaagcaagac ctagacaaat ctacattgga aatcttaaca aaacgtatat tcctgtggac
    65821 gatcgtaagt agaagaattt agtgatttaa ccacggatgc acacggataa acacggatag
    65881 gtagtagatt aagttgacag aattcattta acggttaata tttatttgcc gtttgcgtcc
    65941 gtccccgatc tgtaggtttt aggcacgcct gagccctaca gaatggatga tataagtaaa
    66001 tattacatta ttgagtttga atcatcttat gcaattcttt cgttaattct tttacatctg
    66061 gttcataagc agaagcataa ttttgttttt ttagcataag ccaccaagcg gcaatgaaat
    66121 catatttatt tgtaaatgtg aatgttttta ctaggtttaa atttttatca taaataaaaa
    66181 acttagattc aaacactgcc tcatcccaat atggaatagt tccgtaggtt agtattgcag
    66241 gtaagaaggt gagcactttt aatgttggtt cattttcata attgcttcta tccccataac
    66301 ctgaatacag ttgtttatga tttgcaagaa tccaataatc tacttccatt tttttcaatt
    66361 ggatcttaac taatttttct tgtttatttt ccaaattaca agaacaggta tcttttattt
    66421 ttgcatattc gaaacttaag atggattcta actctggtaa acctgcatgc ctgaagagag
    66481 ataagtgatt caacagaaat tttctgagat tctcatctgg aatagagtta tcgcctatcg
    66541 atactttttg ctttcgcact tcattttcag ttgggaaata tgcttctgtt gatgtatcgt
    66601 atggaattgt tgcgggagca tagtaacttg gctggtattt tccgccataa acagagacaa
    66661 atattttttt gtatagatct ggataggcat ctttttttcc atcattaata aattctacaa
    66721 tacaattgta agtcgcctga ctcttatcaa tatcttttaa cgtatcataa taggaacagg
    66781 ggaaatgagg ttcttctttt tgtatacctc tcataccgtt tgcaaaatga taagggaaaa
    66841 agcctactaa cgcaaatttt ttagatttta gaaattttag ttcttgtttc tttaattcga
    66901 aactggaagt gtctcgtaaa caatactgca tacaaccaaa ttgtaaacct atggaacaaa
    66961 atagaaatag ataaagtttg tttttcatga taataatatc tcatagagta gaaataaaat
    67021 ctatagggga aagccctttc tacggatatc acttacgttt ttaaaatcat tttctctttt
    67081 ctagttttac caaaagataa aatggaaata ggtaaatgaa ttccattctt tgattctaaa
    67141 aaatctagaa agaattggag ttctttcgga attttgtttt ctcttaagga atctaaatct
    67201 tttttccaag ttgtaaattc taaataacga gagggtttac attctaagag tagctttgtt
    67261 aggagctttc tatcttcaag tgaatgcctc tgggggataa tgtctagtat ccatatttca
    67321 ttattttctg atttggtttt aaaaaacttt ctaagtaaat tagaatcttt tccattataa
    67381 atatactcta cacagacttt tattttagga aaatctgaca cttggtctaa acaggtcaaa
    67441 gcaatagacg aaagatcagg gtttaatttc actgcataac gagttaaaat caaatcaaac
    67501 caacccattc taaaacttcc ttgccaatga ttttctagat tgtgctcttc tttgatcttt
    67561 ttggagagaa tctttgtttc tgtaggaaga ggaccctttc catgtcgatg agaatagcat
    67621 cttaggattc caatggattc tattttttta cttagaaaaa gatttttagc gctctctaga
    67681 gtcgttttgg tttttgtgat ataaggaaaa aatccatatt ctaaatcgag tagggttccc
    67741 tgtgctcctt ctagaagata actttcatta cccgcaagta gccttttttt aaaggaattt
    67801 cctttgtcga tcgtgttttg ataagacttt gcaaactctt gaaagaattg aaatacttgt
    67861 cgcagaggat atttattttt gaagtagaga agaattttct ccacttcttt attttctgta
    67921 ttacttagaa ttgaaattgc tctgttgaaa aatttctccc tctgtttttc taatttaata
    67981 agtaagtttt tcgaatctaa atcttttatg tatatggctt cctttggaaa atacatttta
    68041 tcgatagccg caattccgac accttttcca gtcgttccaa ccttattctt tcctctagcg
    68101 atttcttcca tttgtccaat cattttgtgg tagggtgtaa tcagtgtcgc atttggactg
    68161 atgacaattc ttttcaaagc atcctttatt cctttttgtt ttaatacaaa ttcttcttca
    68221 agtagagagt atagatcaat aaacatttgc tcagaaaggt aggttttagt atttttatga
    68281 aatgtcccag aaccaaattg actgaaaata tgagtttttt tatcaggagt tacaactgta
    68341 tgcgctgcct gcgaacctcc attgtagcgt ataacggttt tagtaaagta agcatctact
    68401 aggaaatctg taatacttcc ttttccttca tctccaaatc caagcccgac taggataaaa
    68461 tttttatatt tttgtttttg caaatgcttc cattgccttt aaaaaagctt tttcatttcc
    68521 acagatccca tcactcagta aaaaataaga gtatccaaga gtcttcatca agataaaatc
    68581 agaaagatca gagagggata cttctaaact cgtttcgaga gaggtgaaaa gacgggaggc
    68641 aagaattgct gtttcatcag cttctattat agatttcaat gtaggaaaaa tatgtgattt
    68701 attttctcct atttggatga ataaatcatc ccgtgctgcc atcagtctaa ttttattttt
    68761 taaatttgga aacacttctt taacaaattg gatgcctttt tttgattcaa ttttgcaaat
    68821 gatatttgca ttcgggataa tctttgttag ttgaactata tctgattctt cctctacaaa
    68881 agaaagcatt atattgacaa ttcctaattc ctttgctgca tctaaatact ccaagtcttc
    68941 ctctgtaaaa taaccttgga ttttcaagga ttcatctgga atatttactg cttgtcctgc
    69001 tcctaaagct tttggcggag gtggatctac atagatttta ttgcctttta ttttacgaat
    69061 tgtagatttc tcttcatttc taaaatagat ttcagcagga agactaacgt caatctcatg
    69121 atttaataca atatcaccaa aagttgggac agcccattgg ctaatgcgga gttgccttcc
    69181 tttaagatcg agccagaaat cttttccatt gcagaaacga agaagagttt ctagtgtctc
    69241 ttttggattt gtagaaactc taacgccagt attaaaacga agagaatcta caagaggatg
    69301 atctatcaca agttgtaaag acttttctct atgaggagga ggtagagttg caaagatttt
    69361 catgtgcgtt ttgtctttga ttgtctcttt ttagtagttg gtttttttgc tggaagtttc
    69421 ttttttagat ccacttttgc aatcgcagta gattcgctca gtccagagag agttttctcc
    69481 actagtttga ttctgtctgt agtttgatct ctatcctgca tatctgtaag ataatcatct
    69541 aggctacgag attctgatac aattgcaatt gctcctagca taacgtctac gattgcttta
    69601 gaatcttcta aaactaatat tctctctctg ttcacaacat ttgcccattg ctctacgatt
    69661 ttatcgtcat ccgttccata tcttttgtga atgaggaata cattaaacat ttcatttagc
    69721 tttctaaatg aagatttggc ttctacatct ttttcggtat cgccaatatg cttggtaata
    69781 tgttctgctt ctgtcttcgg ataaaaacct tcatcaccta taaaaaagaa aaatggaaga
    69841 tctgcctttt ctaatttgca atgattggca tagaaatagg ctgttagatc ataggactct
    69901 cttgattgac caccaccgtt atgctctatc caaagttttt cgagccattc atctaatttg
    69961 gttccttgtt caaagtcgca aacttgaatg ggactcatat cactataggc atctccaaca
    70021 acggcaaaac taatagaagg atcttctaaa tatccctgca ttaaaatctg tccataaaac
    70081 attggcatct tgtcatagat aatatatgcg tcatttccca tagatccagt tccatctatg
    70141 gcaaccacaa tcggagattt atttttacat acaagatttc gtttgagtgg taaaacttgt
    70201 ttacttgcac tagtattttt taatgccttt ttagctgtat ctgtataatc gctaaaatca
    70261 taatctgaac tgctagatgt agaacctaca tccctatcat aacttccacc gcccatcgtt
    70321 ttctcctttt ttaaagctcg tcttaattct aggctataac ttaaaattgt aaagataaat
    70381 ataacatgta gtaaccaaat ttaagaaatt aagatataaa taatgatttt gagaaaattc
    70441 aagaatgtaa atgaattagt aaaatataca acttgggata gcttggcttt gggaaatctt
    70501 atcaagatgg atagataggt gactagtaaa cctcatgtga ttttataatt ccatcttgtg
    70561 taaggcgaga ttatagaatc aattctaaaa actgatgtct tgcaccaaag acttcttctc
    70621 ttaaattgga aagcctatgc caaagttgcc acgcatctct tacccttttt ataggatttg
    70681 gatttagaaa ttcgagcaag aaatcttgga ttcgtttatc tacatttgaa ggaaacgcgt
    70741 cagtctctac atctccaccc attaaataga gcatcgattt accgagagaa aaaagatcgg
    70801 acgaaggaat cggtttcttt ttaagagcta cttcaggagc actgtaggat tcattaaaac
    70861 aagaataagt attggaagtg gtatttgcat ttggaatcga aaatagatag tcaattagaa
    70921 atacattatg atcagagggt ctcacaatta tatttcctgg ttcaatattt ccatgaatga
    70981 ttttattgta atgtaggtat ccaagcacac tgagaagcct atctaagatc cagcacatat
    71041 gttctactgg aattccatcg gtaaatttat ctcgaatggt tatcagatca gatccttcga
    71101 tgaatctttc tatcaatcca agttccccgc tagtagttct gaattgatca ataaaaactg
    71161 gtattgattt atgttggatt gatcttagaa taaaaatttc atttcgtaat aaatcgttat
    71221 ccgctttatc tttcataatt ttaacacaga ttttctctgg atttgagttt ttatttataa
    71281 attcacccat ataaagatct gcataatcgg cttctagaaa ttgttcaaag attttataag
    71341 ttctaagttt tgttttgatt tcaaaagaag cagttgtatt tacacttaga tttgttttgc
    71401 cgtagatatc tttctcaatt cttttttttg cttctgcata aagaccatta ataatagagc
    71461 tgatttctcc tgctaagtat tttgtaaaag cgtctttttt attaaattta tcaggatgaa
    71521 aagttttaat taaatcttta aaaatttcat ttagcgaaat taatttattt tgaggtaaat
    71581 catcctttat ttctccgaac aattcttctg gagagctaaa ttgaattatt ttggaatgaa
    71641 attcctttag ttcttttgtg tccaagaatt aacctatttg atttgtttta tatacttttc
    71701 aatttctggt ccaaggatag tgattagttt tttataaatc tcttttttaa aatctacaat
    71761 tgtattggct gcatttttta agggaaagaa ttggactgtt tcaaattctc tttcatgaat
    71821 atctaattta cattcagacg cagggtgatt ccaataggca agaaaccatt tttgtgtttg
    71881 tcctttgtaa tctttcatat gcggtaaatc tagatttgcc ggaaaatcgt agctcaacca
    71941 ttctgggact tcatagataa attctgcatt atcaattcca acttcttcat agagttctct
    72001 tttagcggcg actatcggtt cctcgccttc atctagtcct ccttgcggaa attgccagga
    72061 gctacggtga gagattctgt ttcctaataa tacaaatcct tttgcattaa atactacaac
    72121 gccaacgttt tttctataac ctttcatgaa gtctctcctt ttctatattc aatccgcatg
    72181 agagataaat catcatagat ctcgcctatt ttttcaatct cttttaatat taattctaaa
    72241 tttccttttc ctttctcgac atgggtagag aaaatatcaa agttatcatt aatatctcca
    72301 ttgggaagta gaaggtcatc tcttccatct gagccaataa tcaatacatc atggtcttgt
    72361 agctgataat aatttaaaga cacagtctct tccaataaag gggaaccgac cttagtaatt
    72421 gtttcatcta acggtagaaa attagctttt tcatctctat acaatacgat agctggatgt
    72481 tctgcattta tataacaaag ccttcccgtt ttatcctcaa tgatacccat attgagagaa
    72541 atcatcatag agccatcaaa cataataaaa acgttatgta attcattaaa agcatttata
    72601 agccagtttt ctggtgtttg gtttgcaaaa aaaggattca tgttagtcct ttttacaata
    72661 ctttcaaaaa ttgaatttaa aactagaatc cctccagcac cttgcattga ttttcccatt
    72721 gcatctgcat ttagaaataa tgtcatactt ttattttgaa gtgaaattgt atgagcaatg
    72781 caggtatctc cgccaatctc catattttca ttgtggaaag aaaacttctt tttctgtcga
    72841 attagaaagt caatgtttat atttggacta gaaatggctt ttacggttaa cggctctaaa
    72901 agtagatatg taaggaagta atcaccatcc tgactgagtt ttaattcatt tactcttgtt
    72961 aatgcttttt gtaattcttc tgttctaaga attacttttt tctctaagtt ttgatttaaa
    73021 tcttctactt cattgtgaac ccttacaaaa cgatttgata gaataaaagc cagacttaaa
    73081 ataaaaacaa agaatccata ttctgtaaat ctttccattt gaaagaatcc aagatggatt
    73141 agtatatcaa agacaatact agaaataagc attgaccaac ctgcaaacat gattttggca
    73201 tcattattcc ctttgaaaaa tgcgttatat gtaataatta aaaaaatgga gattaataga
    73261 ggagtccaca ggaaaggaat tacataagca tttaaaagcg aattccattg atctatatca
    73321 gtagtaaaga tcatataaat aaagagacct accaatgata agagtggaat agaattcaga
    73381 taatgaagtc ttcttaggaa ttgattttcg gtggaaataa aataatatct gaaaaaaaga
    73441 tataaaaata ccagaagaag tattaagatt tgaaattcca cttttttcat ttcaaaatag
    73501 gtgaatccaa actcaaatcg caactgattt ctcagagcgt ggtaagcaac gaaaccaaga
    73561 gtgaacagtc caaagtaaag attttctctt tcctttctcc ttcttaaaaa gagaaaaata
    73621 aaataggaac caactgaaaa atagcaaaat agaaaaataa gtggttttat atttgataaa
    73681 taatactcac gtaggatcgg tttgtttttt cctataaggt tctgttctcc ccagattcct
    73741 gcactttctt taaaatattt ttttacatga atcagtaata tattcggtgc gtcttttcga
    73801 aaaagttctt tattgattga atagattctt gctttatcgt aagcttgagc tttatcactt
    73861 tcccaatctc cggttctacc aattaacgtt ccatttaggt aggtctcatc gctatcggat
    73921 attctctgga tgtaaagaac ttggtcttct tctggaattt cctttaaaat gaaaactttt
    73981 cgataccaaa aatactttga attctcattt acaatatttt ctgctaccgg attgccagga
    74041 atatgagtgg acacccaaat tttatcgggt aaatcaaaag agttcgtctt tgaaagcgga
    74101 tcattactat ttgccttggg atttatttct tgagatgttg cccaccatgt atgtatttta
    74161 ctttttggtg agtttaggtc agtaaattct ttttttaaat ctatcgtttg aatctcaaaa
    74221 ttttgagaat aaatcgatat cgaaaaaaga atgagtagta gaatgagttt catatctaaa
    74281 atctactctt cctcaaggtc atcatcctca atgtcttcat cgtctgaatc gatttttgta
    74341 ttccgagatg aattctttgt ggaaggaggc aatactatat ttgaatttgt ttcttttttt
    74401 tgagaagtga caggagtagt tgaataaatt ttcaaaattg agtcttgaat tgttttcctt
    74461 gcagttgcga gaggagtatt tccttttttg tctttacttt ctttatctgc tccagcggca
    74521 actagtagtt taacgatttc aagattttct gttttgattg cataatggag tggagtaagt
    74581 ccttctttcg ttttagcatt tatattttcg gaattgagta gaagttgaac gatttctttg
    74641 tttccctttg agcatgccca gtgaagagcg gtaaatccat cttttcccct tcctttgatt
    74701 tcctcactga ttaaattgtt taactggatt aggttttcta aacttacttt atgcaaagta
    74761 gaatttctag gagttgatcc agacttttta tgaagattga ttaaatcggc aatttgatta
    74821 tagccttttc tttgtgctat ttcgagtgcg gttccatatg gataaccatt cgattttata
    74881 tttatgtctg ctccattttc tagaagaatt ttaactacag ataaatgtcc attagccgct
    74941 gctagtatga gtgcagtctc ttgattattc tttgtcttgt agttgatgtt ggcttttttt
    75001 tccaggagca aggaaacgat tttttcattt ccattctttg atgcgattag gaggggtgta
    75061 tagtttttat tgtaactctc ttctacgatt agatcgattt ctacagggta gttcagaagg
    75121 atctttgccg cttcagtctt tccaaattca cttgctagat agagtggaga tttcccttgt
    75181 gcattcaaaa tgtttagatc cacttttttc ttgattaaga actgaagtaa ttttaaattt
    75241 tcagaaagaa cagacgttat aagaatcgta tttccatttg catcactgct ttctagatta
    75301 cttccttttt caattagata g
//
LOCUS       DAOQOS010000007        73849 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_115_length_73849_cov_6.856235, whole genome shotgun sequence.
ACCESSION   DAOQOS010000007 DAOQOS010000000
VERSION     DAOQOS010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 73849)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 73849)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..73849
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_115_length_73849_cov_6.856235"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..946)
                     /locus_tag="PLF66_02545"
     CDS             complement(<1..946)
                     /locus_tag="PLF66_02545"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020777167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HMZ62945.1"
                     /translation="MERTVENEVVGKQSLSLSLQTKIYKKLFQNALSKMKLGSMKIVD
                     PDGTAVLYGDTTKQYESFNSAHILVKSYDFYRKSVYYGDIGFAESYLDGDWETENISE
                     VISWFILNVEDSPNLSGTKKSSRRLRLFNFFNRLYHNSRANTVTGSKKNIVDHYDLGN
                     HFYKLFLDETMTYSSAYFKTNKETLEEAQILKYDALCQKIKLNSSHHLLEIGSGWGGF
                     STYAASKYGCKVTTVTISDEQFKYAKDLIHKKGLENQIDIQLCDYRYIQGKYDRIVSI
                     EMLEAVGHNYFETYFAKCNEVLKKNGVIGLQYITCPDSR"
     gene            complement(930..1709)
                     /locus_tag="PLF66_02550"
     CDS             complement(930..1709)
                     /locus_tag="PLF66_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010572523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1365 domain-containing protein"
                     /protein_id="HMZ62946.1"
                     /translation="MEINSSIYECKVAHNRIIPKKHAFSYGLFTFSLDLDEIDILKDK
                     YLLFSNESWNLFSFYKKDHFQYGENDFKSGIIQYAKENGCNANIGRVVLITHLRILGY
                     TFNPVCFYYLYDKENVPVCALVEVHNTFGEMKPFVVLNPNGDYRFYLRTIKHFYVSPF
                     FELDTEFEFKLNPPTEKLNIHIDDYKEGQKVFLSAYTGKKKEMSDKNMILLFLKYPFV
                     TLKVILMIHWQAFLLFLKKIPFIRKMENIHMQKGVYLGKNS"
     gene            complement(1700..2953)
                     /locus_tag="PLF66_02555"
     CDS             complement(1700..2953)
                     /locus_tag="PLF66_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HMZ62947.1"
                     /translation="MTRLAIIGTGVAGMGLGYFIHKDFEITYYDKNDYIGGHTNTVDV
                     AEGNKLLPIDTGFIVYNEVTYPNLTRLFRELSVETKNSSMSFSVQHLPTHLEFCGSGL
                     NGLFAQRTNLFSVKYLKLLYQINRFNNESPQILEDPKYKHYTLKQYILEKKMGEDILY
                     RYLIPMSSAVWSTDERDMLEFPAVTLVRFFYNHGFLGLNTQHQWRTVVNGSRSYIKKI
                     IEPFKDRFVLKNGAKKVIREANRVKLILQDGSVEYYDKLVFACHADTVLALLENPTQK
                     EKSLLQSFRYQKNIATLHTDESVMPHKKLAWSSWNYKVENNGDSSVVYWMNSLQGVSK
                     NKNYFVSINDPNQVNPQKIIRKVEYEHPVFNVSSIDAQVDLPSLNEGNLIYYCGSYFK
                     YGFHEDAFTSALDLARTLLGREIWK"
     gene            3091..6552
                     /locus_tag="PLF66_02560"
     CDS             3091..6552
                     /locus_tag="PLF66_02560"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HMZ62948.1"
                     /translation="MKQKFPCNEMSSECKYNFDLHSNQHVIIEASAGTGKTYTIENII
                     PQFIENNISIESIAVLTFTEKAAAELQDRIRKKLLNSLLSSQTNSKLFENAIESFPNA
                     NIGTIHQFCRSILKKFSSELGLSSNFTEIKDTTTEIETYFKKFWGKIEKDDSEELIQL
                     LQIIDYSVFKDIIIRIAVNTSGQIVAPLKDFFNEDLCKSVFAKIKNLIQKVPFEKKMP
                     DYKVRLKSLLDLNESLPNLEIIVAYEQAFLKDDHNPNTNVKKFLLKETSISESEYDSL
                     MDSLSTHICNFKQKEYLPFLNQILNLTNQFISEYKDYLQSKDKIQLDDLIFQTRRLIL
                     EFPEIQKQLQKTYRYLILDECQDTNPIQIDLILELFKNKNAGIILVGDPKQSIYRFRN
                     ADLDSYQDSIKKLNTQDYIQLDTSFRSSERLIQAFNFIFPHFANLQNIYKPVKASRSL
                     DPIAKELKPPILFLGLDENNIPRTTDKSGEKFSADSLREDSLREIIQAIKTITHNENY
                     KIQIKNTNDYRTIEYSDIAVLAKTNEDLNNILQEFSKHSISANIYKSVFFYSHKIVQA
                     ISHLLHAIENPNDTSSIYKALASDLFLIPERTLFLLSERKELNYLIPTSFPEIEEIYK
                     TLRKAHEYRYTQDIALTLHNLLTENRILEFISMGFDGKRNLANIYHLLEILSYSQLTE
                     NMSFGEIVRNFKKDVEKATDQEIKLDNDKKDFSFNSVQLMTYHASKGLEFPVCILYNL
                     AGGSNPKNKTTIYLRDKTLKQNPIGVELQIKNKHGKKWNSPAFDTFENNDEQSLLEEK
                     DRVLYVAFTRAKDYLILPLHLPAPSDSFRNRISSAFSNENINSLIQLKLAENFHYPLE
                     STKKDVNISSQDSSTETINKQIRKIDLTPNEIYSKSGIRLQSYSSLAKHLESEEESAK
                     EKVASEKKSYLESEESSPLAISSEKRGTTFGLLCHAVMENFDLSLLSDSLATRQAVTE
                     MVNQYYPSTGLMETKDYTSQEAIEYCYSTLTKSYPMNLEKTEFAFIKDWQHLKRERSF
                     YYKLKSAKMDYLIGIGDGLFLWKNKYYLLDWKTNLIHTEDKTIDEVIQEKVEHSYRYQ
                     YMIYSMNLLDNIAYKSLDQEKFWEENFGGMLFIFMRVTEDKKGSLLVKPSFGELQKFK
                     KELEELK"
     gene            6549..8252
                     /locus_tag="PLF66_02565"
     CDS             6549..8252
                     /locus_tag="PLF66_02565"
                     /inference="COORDINATES: protein motif:HMM:NF021375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HMZ62949.1"
                     /translation="MNPSILPGIIRGLGKARIQFDKAYENRKILSLHNKQISILEKID
                     FKNKDFSKSSVFKASKLISDSVFDEVIFWTLLKLIHSEENGGICIPKNDLVSFIKNQN
                     PKLQVEIAWQKYLDQSGDNSLLAFEGEMVYLKSLYQTESFVCDKLKLFFETSFPEKFK
                     LFKDKNLSAEQIQIINSILKNGISFVSGGPGTGKTTIIQSILKAAIENKINPEEIALL
                     APTGKAAKRLQESCQTLIEENPSLEPSTIHRFLKYSPKNGKFRFNSENPIPHKLIILD
                     ESSMIDIFTLKALLEAYTNEDKTKRIVFVGDPDQLLSVNTGSIFSDFISLNRNLYTLT
                     QSFRQSKEGEDIKTFANYLKNLYKANHTPLPEIITIEKNITNSKNGVRALDLTKNAEI
                     LNHALEWYKIHSQTKESIQILTPYNETEIGIKQVNSYLEENLPTQSKIPVIVRQNLYN
                     LGIFNGETGLLEQAKTHSLFYPANKEKIEISESNLQYFNSAYAITVHKSQGSEYDHVC
                     LLLPAELEEPDSLLNIRIIYTAITRAKKSVTIIGSLSLFQKSLLNKGEERHSRISNIL
                     K"
     gene            complement(8407..10137)
                     /locus_tag="PLF66_02570"
     CDS             complement(8407..10137)
                     /locus_tag="PLF66_02570"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HMZ62950.1"
                     /translation="MADLKTLGKYYLLSSLPVLYSLSDEDKSYIAEKAILKDLKRNEY
                     LYKQGSPFDYFYILVNGSIELVNHVRVDNEEKQVSVEVMRKGDFLGVVSMMGGKPHTF
                     SAKALGDVKLLLLSQEDFNDVMNRVPALGVVISRVLARRLKASPDESDRKIIESNVIA
                     VHCDKNPSLGAHFALELSEHIINETGKKSIVLRFNKKKEHLIHASSKVKNYDFDDIDA
                     ILNQYHKYRYSYHFIILDLPEDKPELCKVLLQESDHCFLITEPELKDKSVFLKQYELE
                     NTNDVNFISNVILKESIKSSELNSIARKLARKISGMRLGLALGGGAAFGLAQIGVLKV
                     LERENITVDLVSGTSIGSLVGALWASGVSASEIEKATEEFDSLFNMLKLMDFSIMPTK
                     GLISGNNIRKFLEGFLGHRTFQELPMELRTITCDINTRQEVISKEGSVVDAVMASTAI
                     PGLFNPLVTPKGVYVDGGVVNPLPVSALTIEGVQRIIAVNAMPSPEDVVKSNKKDQSL
                     MDIFINSFYSLQYRLCKYSFQSSDVYMSPILPNSSWYEFYRAKEFIALGEKVCEENLK
                     EIKNLAFKSS"
     gene            10202..10327
                     /locus_tag="PLF66_02575"
     CDS             10202..10327
                     /locus_tag="PLF66_02575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62951.1"
                     /translation="MKENRIQSIESGRIHRKITPIEEIKTGNNSVSKRLFRKHTN"
     gene            10581..11771
                     /locus_tag="PLF66_02580"
     CDS             10581..11771
                     /locus_tag="PLF66_02580"
                     /inference="COORDINATES: protein motif:HMM:NF019245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1577 domain-containing protein"
                     /protein_id="HMZ62952.1"
                     /translation="METKEITDAASIFKMAEGLFKQFPVYTIVQGKPQQVKVMGMKNQ
                     AIVIQTPDENPNPTQRLLIMTNAGNLLQFSFKVNTKDPRGLEVLNPNTLTIKPASRVS
                     NRYQANKAPILITNIISQTMIPHDLADDTMKVESLMKPYIQKLKGKYSEVEFFVHERL
                     DPRTRLLTETGKHIFVPDTKNPESVSDQFIPYSEHVHLMRQGKGLSKFISEICVPLKY
                     RGIFIYGYLQVLHLEKADMNDYNLIVAAAERFCKDIENAGIFNESKLKSSILDISGNG
                     FSFSHPQSKHFGKIFSIGGTILFDMYEDEKSLGTFRAVVRNIKPLEKLFRIGCQFFHI
                     SDEHKIIDDLMQKFFPQSLESQSQEADNAPKTEKPATDPKLPAVNQPVATETTEESAS
                     TETN"
     gene            complement(11768..13807)
                     /locus_tag="PLF66_02585"
     CDS             complement(11768..13807)
                     /locus_tag="PLF66_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016551275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HMZ62953.1"
                     /translation="MKIIYLFILLFSFSFSNLWSQTNTDSKDAKTIETKDTKEVETKD
                     SKTTETKDLEKDKQRLQEIEANLKKQQQELEELKKQYGVKSESLNQNSEDNYVSPLKT
                     KGLTGEYNRSMFLDPDVAKKFNKDQKPWLNDWVRVGAYVRPRYEDRYNLGFDKQNKGY
                     ISRAMQTTQLFFLMDPSPYFTMKITVQDARVLGGSTAAVPGDNRGPTFAGVGNTITPG
                     QGQTIPIQTTIREAWFMAKKLPLDAKLQVGRQILVYGDQRLLGGANWTINGLSYDGAR
                     LMFDQDSFNIHFLAYKLGASQSGPNGVFTSGSSVSYTDPVSGKSSVITPAQPNQYLVG
                     TYDTVKFKNLFLVDLYSLGILTQKTSSFNGAILTNPSLTIPATADSDLYNNSWSKQKN
                     NLWTTGFRISNRTANGNLADESPWGGWDWTFEGAIQSGTTGARVNKNPSLNSAINSIY
                     ATTLGTTTPSYNWLAQNQKYAGRFFVFQTGYTFAKKFRIGGQYLFASGDSNRTDGSNA
                     TFQTLPNPRFGVIPYWNNVTGLSENIDAKNLISKTLNLSYKTDKWGTFMASYFWNDKD
                     KTQDAWYAINGTANTGATTEFNNGQTTLAFAKSSRRLYDELDFTWMYNLNDHVSLWIG
                     GGMLTAGKAIRNQKNAAYSYNTHTGSISQNNAVLLGQSGAASKANMFFFQLNASF"
     gene            complement(13959..14312)
                     /locus_tag="PLF66_02590"
     CDS             complement(13959..14312)
                     /locus_tag="PLF66_02590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62954.1"
                     /translation="MSDCFLIRLLFRILFFGFAFFLFLENPISESEYLPHSNSFTDQK
                     YYSNTAEAFRENYFAELEEEENLLTHCIFFAAIKILISFNDNIQCHKRNIIYQFQIQS
                     HLINLPPPIDMGVVS"
     gene            14492..14890
                     /locus_tag="PLF66_02595"
     CDS             14492..14890
                     /locus_tag="PLF66_02595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62955.1"
                     /translation="MMKILLSLFLFFITSYLFAIEIIPYKKIDETLKTTWDNTYPVEF
                     KKVIKKDLLGKGIMVTKNKQKQLEYVYSFLVFIPRVSMVEGVKTEMEDGKEIIVKLVH
                     NPSDTEKPYRIELGEFSEKYYQGNVVKWIK"
     gene            14878..16290
                     /locus_tag="PLF66_02600"
     CDS             14878..16290
                     /locus_tag="PLF66_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004788150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HMZ62956.1"
                     /translation="MDQMKHEILIKEKMKAEGLSDLLIQDFLTKVEKVRTGETGKVKW
                     ETIGDLDPEKDEIDLEVLRKKYPIKKETLAKLVVIKLNGGLGTSMGLEKAKSLIPIKD
                     GMSFLKIIANQVQYIRSKFGVEIPLILMNSYNTESDSLEELKKAGFKQDITTSFLQNK
                     VPRLNKSDLTPITLSDKKEEWCPPGHGDIYLSLKQTGILDQLLSHGIEYAFISNGDNL
                     GATIEPSILEYLVDQNLEFAMEMTPKTLADTKGGAIYRKMINGKFVGLELLETAQVPK
                     EHEHEFSGMGKFRTFSTNNLWVNLKALSALMKEKTPSLSLIVNPKVVDGKDVLQLETA
                     MGSAIGSFQKTKGIIIPRDRFAPVKKCEDYLIRRSDAYVLNEDYSLTMNPERKKAGLS
                     ENLVSLDDNFYKKIKSFEKLFPAYPSLVYSNSLKVEGEIEFDAPIVLKGDVTFKNKSG
                     SLKKISSLGRKEFKDEVVEL"
     gene            complement(16431..18452)
                     /locus_tag="PLF66_02605"
     CDS             complement(16431..18452)
                     /locus_tag="PLF66_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020775911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HMZ62957.1"
                     /translation="MLLLYLLTFYTCKDTVNKKNQPIAEKGILDLRNWNFENDGIVSL
                     DGEWEFFWEEFLEPSVFNIPVENQIIIRQPNRNKINILKSNLIKVPSAWNGYIINGKE
                     LSGDGYATYRLKLLVNGPVPLAMRIPTIASAYKVFINGLLIAEDGNIGKTKEEYYPYQ
                     NVRIIQITNPSTEYEIVFHISNFDHRLGGLWSKIELGESESIKDRREKYLFRDFILSG
                     SFLIMGLYHFGLYSLRRKEKSVLSFGILSLAVSFYILFSGEFYAYNLFPNLNFKIGTK
                     IEYITVYIGVSVAILFFDQIFSSEKIEKISIVIQRLPLFLTIPTFLFSIKIIDYGLIL
                     FQITTLVGLIYIIYLLSFAIYHKREGAKSYLMGFVIFGTCTVNDILVGQRIIQNDLYS
                     PFGFFIFIFSQAFLLSTRFARAFSQVEDLSANLERKVIERTNQLNLALQKSDKLLLNI
                     LPEEVADELKEKGFVTPVLFESSSIMFTDFKGFTQIAEGLTPRELIKELDACFTQFDK
                     VTERYNLEKLKTIGDSYMCAGGIPKVNTTHAIDSCLAALEIQSFMNRMKSLKQEINAP
                     YWELRLGIHSGPVMAGVVGEKKFAYDIWGDTVNTASRMESSGTAGKINISYTTYELVK
                     EIFDCDYRGEVEAKNKGKVKMYYLNRIKKEFSKDEQGLVPNGKFWNKFV"
     gene            complement(18476..18862)
                     /locus_tag="PLF66_02610"
     CDS             complement(18476..18862)
                     /locus_tag="PLF66_02610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62958.1"
                     /translation="MMVAPNTRAISGIKKNKLKQAILERFANKDVLVHNYLIWNSFNQ
                     KPKVSKSKHELAVLPKNITIKPSQSLFFFNERLDKFYAIKYSEFLKIIRSLEPWEELD
                     AYLFDESMQWIIAHTHDRDILVIGNP"
     gene            complement(18862..19332)
                     /locus_tag="PLF66_02615"
     CDS             complement(18862..19332)
                     /locus_tag="PLF66_02615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62959.1"
                     /translation="MNHFASNLQTKPRKKKTQFVWVIATILFTLGLTVYFTNSVIDIY
                     DAKKQNIPKKTSRKIIKKNSKEWKDAVKRIKDSKGKGNNATVKNKETAKELILEAKPD
                     LAEYPMYAKEPYKSGYEFHPAEPVVGNDLPHIKWKDWSNGKSDGSEGHIFYEEE"
     gene            complement(19557..20597)
                     /gene="queA"
                     /locus_tag="PLF66_02620"
     CDS             complement(19557..20597)
                     /gene="queA"
                     /locus_tag="PLF66_02620"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HMZ62960.1"
                     /translation="MIDLTEYKFDLPEELIARYPVDVRDESRLMIVNRNTNSIQIEPM
                     FKNIINYLDSKDLLVYNQTRVSKRRVYLRSDSGREFETIFLENKNSDKKTWKCLVKNV
                     RKLKQGDTLVSIDPSISFRLERNSEGDIYLFSAIEITEDIFEKIGTIPIPPYLKRKAD
                     AKDNERYQTIFAKSSGSVAAPTAGLHFTDELKSEIQKKGATFTEVELCIGYGTFSPLT
                     EKQIQEKKLHEEEFYIPEKSFHFLNEAKKDHSKRIIAIGTTSLRTLESSYDKSSKSFL
                     RKQGITDIFIQPGDLVESINGLITNFHLPESSLLLLVSAFAGRDLILEAYHTAIKEKM
                     RFFSYGDAMLIL"
     gene            21170..22441
                     /locus_tag="PLF66_02625"
     CDS             21170..22441
                     /locus_tag="PLF66_02625"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HMZ62961.1"
                     /translation="MLERIKKFSPYLLGIILLVLLVQSIGRADNKSDFHDYYTASRLF
                     YDQKDLYNINALESLREEVKLEDLFKAENLKKLEALKGNLGTYIYPPLFAFLLIPLGF
                     LNYELAATIFTLINFSCLLLSLFLITKLIPIKNIFSILFFTIAVNYRYLESHVSNNQV
                     AFILILLILLSLLIKNDALSGILLSLAIIIKITPAIFLFYFLYKRQFARFFYTFLFAG
                     LWLLLPSLYSHEYNLHSLQNWYDLVLNNAMKNPTFRSWKNNQSLIATLAKFFMVGADP
                     LNQALFGMPYLNLSARTLTYLFYFCSLVVGTPFLYKLKEGFKDANLISILFILSVIFS
                     GISWVHSFVFLLFPIAYLSQQFFEENRNESKQKIFYALCGITILTSRGIIGSTLEGVF
                     LMFSILLYISIGFYLLLIKIKTDDTTNEFKV"
     gene            22442..23593
                     /locus_tag="PLF66_02630"
     CDS             22442..23593
                     /locus_tag="PLF66_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016545711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HMZ62962.1"
                     /translation="MSMQLDVKYKPRVAVDARPLAYGLTGNSRYLFEALKLLIRKDSH
                     FEYYLYSNKEIHPMFMDFIKQNEVYVAPIKNVTGVLWLNFILPYMMYNDRIDIFWGTL
                     QLLPFIKLRIPEFVNYHDLNFRSAPETMTKANYIQHKLLSPRSLRNADLVFCLSQNTQ
                     REIAEFKPEYTKKLKVIYPGVSKKVITNNTLPFSGKYLFTIGTLEPRKNIATVIEAYL
                     LIKKDFPDFPYKLVIASRRGWGQQELTQKLISGELEKDGVVFVESPSDDLLNTLYQKC
                     SAFLFPSIHEGFGLPLLEALLEQKVCIASDIPVFREILDLDSDILANPRVTEEWKNAI
                     LKITEQDPIKRKRKWNEKQWTWLATASQIEESFLIEWHKRLSSSVNNAV"
     gene            23583..25067
                     /locus_tag="PLF66_02635"
     CDS             23583..25067
                     /locus_tag="PLF66_02635"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002179700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HMZ62963.1"
                     /translation="MQFNSIHFIIFFFFSLILGNILKNTWQRVFLLLASVYFYTYTNT
                     YFLALLILSTLIDYFAALAIDNPIRTEKQRKIFLGISVFFNLIILAYFKYAYLTTDII
                     NQIFGSSLRTFNIVAYYVNLLHLTPNPQDVFLEKIVLPVGISFYTFQSMSYTIDVYRK
                     QMEARKSFIDFSLYVAFFPQLVAGPIVRATTFFRDLDHRLSVTNEDIQVATTRILIGF
                     MRKLVFADNLGKVVNHTFFHYQTMNAIEIWTGAIAFGWQIYFDFAGYTDIAIGVARLF
                     GFKFDPNFNFPMSTTSITDHWTKWHISFSTWIRDYIYIPLGGSRGGAWLTYRNIFITW
                     LFGGVWHGADYHYIAWGLWQAVMLSIHRFYSQTKLREFLNTSGGFLYKAFSGVFTLFC
                     LAFGFVMFRAQNDPNGTAMHHVYEMCKSMLLLTDHSRVFDSYSNYEFGILLVICYLAS
                     GYFSKRNIEFMATNGNKLLYANVVAIFLILLFGAETQTFMYFQF"
     gene            25075..26250
                     /locus_tag="PLF66_02640"
     CDS             25075..26250
                     /locus_tag="PLF66_02640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HMZ62964.1"
                     /translation="MTEFNKFYKDKRFYVPILILIGFEIFFQLFADFYKPYLKKKSYA
                     ANINQILGHIIDKKKEHDPDILIMGTSVAYQGLSLPVLQEKIKDTGYKIQSVAIPGSE
                     LIVQSLAIEKALREFQNVKLVIYVGEITMPWVSKKDLTPPTLAMINEFDKKDSFPKVI
                     DFGYDAGEGLGFLLHYDNWSYLLFKSIAYRRDLSDFIIDPAKRFKYLARKKANPNLNF
                     YEYENDRSEKMSDYHITSIDDCLQKTNFEFNHDPIPPTSNFDHKKAIFDTCWVGKFST
                     PAQTRTAESDLYFKRLSYIYSHIRSKNIKIINIFAPYSTSVENQLGGAGRVQIWKEEL
                     EKLNPPNPILIDFRSVFNGKNSDDYCYDVIHLNRNGAILFSEALGDYLKDNVKKIIQ"
     gene            26260..27660
                     /locus_tag="PLF66_02645"
     CDS             26260..27660
                     /locus_tag="PLF66_02645"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000578215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HMZ62965.1"
                     /translation="MLFTSLLFAAFFLLVYIVYWSIPNQKGKEIWLLLASIVFYGSWS
                     FGFLFHFLGMIAINYYFIRILYDNQSKKIITLAVIINLINLGIFKYFYFFTDIFASLS
                     GIAALADLKKSTTFKIILPLAISFYTFQMLAYIIDVYRKKVSEKISLFDFTIFILFFP
                     QLIAGPIMRSQDFLPELRNIKAKREYLTPGLSFLALGILKKVLLADNLALLIDPVWSN
                     PAKYEWTTLLLAAHAFSWQIYCDFSGYTDIARGCAFLLGFNIPENFRSPFLADSARDL
                     WQRWHITLSTWLRDYLYIPLGGNRQGEFRSYINLILTFTLGGLWHGANFTYIIWGFYH
                     GVILAIERACERFNIRITSTNFLWKLVCILYAYHIFAIGAAMFRANSVADFITMFSGI
                     FQGASGIAMSKTDFETVLYLCLFSLFIQIVQYFEKLPIWVVKSRQVLIPLSYVILLYL
                     ISLYSKSGKEFVYFQF"
     gene            27664..28740
                     /locus_tag="PLF66_02650"
     CDS             27664..28740
                     /locus_tag="PLF66_02650"
                     /inference="COORDINATES: protein motif:HMM:NF019243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1574 family protein"
                     /protein_id="HMZ62966.1"
                     /translation="MNETFLKHKFLIYPLLLASFIFCFDKLFGTDFVRKYTESRIEYA
                     FYAEKGALLAQLLEFQSKKKPNEKLLVLFGTSHFGEFSHKYIQEKDKRLTTYNFSAPM
                     APPSYLYYNLHILLKAGVKIDYAVLEIIPEIFQDAANEYALKFSYDWKFMFENRDIFS
                     GSELESYTHANLFNVIRFPPRLNIAMQRIKDKSKIDAFEYFNSMVHLATKQNNGGIPN
                     PIIHEVPEEFFEKESNVYFGQAFRKQGNQTFVESNVQRKFFEKFIETCKQNNIKLLVY
                     KPIVSKSLQKLLDESDFYANWWKEKSEFAQKNHIPMLNMVDYTNSIQCQKFVDVHHLS
                     GGCYPEITDILLEKILPQINDEHR"
     gene            28727..29113
                     /locus_tag="PLF66_02655"
     CDS             28727..29113
                     /locus_tag="PLF66_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020772386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HMZ62967.1"
                     /translation="MSTDKNSNPDQDIATNNADLTLNQAQAQVDKWIKTIGVRYFSEL
                     TNLALLVEEVGELSRLIARTYGDQSFKKSDLEKDIPSEMGDVLFVLICLANQMGISLE
                     DALNKTIQKNTSRDKDRHKENKKLIQ"
     gene            complement(29271..29591)
                     /locus_tag="PLF66_02660"
     CDS             complement(29271..29591)
                     /locus_tag="PLF66_02660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62968.1"
                     /translation="MLLKFILFISFFILPLTAEVNNQWGKEFGSLTWADANKKCQENG
                     MRLPTKSELLSLYKTREFKKWNENWYWTKDELDSERAWAVVLNVGTILHIPKQYRNYV
                     RCIY"
     gene            29712..30476
                     /locus_tag="PLF66_02665"
     CDS             29712..30476
                     /locus_tag="PLF66_02665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62969.1"
                     /translation="MSHKHEEKEFELKSIANLLLLPEKEILYLDKLESNEINRLRQQI
                     INSVEEGQKQIWIPMAKVSKFMPNFLNAKVTEDILGPSIAANLSYHLPAKDALGIAAY
                     FSIPFFCDVLEHLVPEKIVDMIKQSPFDLMRRAVNELIKRKNYFLIGSLIDYTPTESV
                     DKIARGLSNLGDLISITYNARDKSRVLGLFKQFDDAKILGVMQAGLNTELANELKTIY
                     EHADEELLDKTLRIIKAKDPNLLEKYNDMLSHFDKD"
     gene            30491..31018
                     /locus_tag="PLF66_02670"
     CDS             30491..31018
                     /locus_tag="PLF66_02670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62970.1"
                     /translation="MSQSTSNKRYTLDSLAELNSDELESLYRIGTVPDDFRSVHGKPK
                     GRMLTVRPTDGTPIFHVLKQISAHGAFPWDGKSFSYSGKQTGQGINRVKLLFSNFDWF
                     PFEIRVEPSVIDDKPCIFLDYEQPENPWFIAKIHDEIREVSPGIYMGPAMWKEDKSSP
                     ILVLWFALDFNNQTV"
     gene            complement(31159..32457)
                     /locus_tag="PLF66_02675"
     CDS             complement(31159..32457)
                     /locus_tag="PLF66_02675"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020777232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HMZ62971.1"
                     /translation="MNTKVDKFGECTIPNPAGYEFYTKENSVVIYQSTFENPESMKNA
                     FNENPIYFEQSGPLEKIYFNPSKVTAGIVTCGGLCPGLNDVIRGLVMELHYRYNVPRI
                     LGFKYGFEGLVKKFGHKPIELKPDLVSNIVRDGGSILASSRGNQDIGEMVDFLGLHGV
                     NILFCIGGDGTLRAANAIYEEVKNRGEKISIIGIPKTIDNDINMIHKTFGFSTAFAEA
                     MTAISCAHVEAKGAPNGIGLVKLMGRHSGFITVNAALASKDVNFVLIPEQDFDLEGKG
                     AFLPALKERIIRRNHAVILVAEGAGQKFFDNNSETDPSGNKKLGDIGIFLKNEISKYF
                     KKENIPINLKYIDPSYIIRSVPANAEDSVFCNFLAQNAVHAGMAGRTGMVVGIWNNVF
                     TNIPITLAISERKVLVPERSSLWRSVVASTGQPNSMKARS"
     gene            32625..33500
                     /locus_tag="PLF66_02680"
     CDS             32625..33500
                     /locus_tag="PLF66_02680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62972.1"
                     /translation="MKKSSLKMIAITSLLLLTEVFSENLKTNPTNKKFDSVDQSDWVI
                     SHNFYDGSYYSQAIVIPFNYPFEASLIPGATKPNNLNSIDFISKFNFSTPSAVSSGKV
                     ILKPSENTELAAYNSNIVTDSNQILRDTNLRMPSPWQRNSTVANTTSGFVFAYLKKNI
                     GFNIDLSLRMIGNEAGQAVLDSVSSGLSFSYRISDRVFQMNPNYRLNLIFQVSSMQYT
                     DERAMPKKLEFSKNLRSQFISSGFTLNTRSVMFEGLIRLPVHQQAMFDLDGLLRPELQ
                     GRLGMKWYLPEYIQP"
     gene            complement(33653..34297)
                     /locus_tag="PLF66_02685"
     CDS             complement(33653..34297)
                     /locus_tag="PLF66_02685"
                     /inference="COORDINATES: protein motif:HMM:NF024852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HMZ62973.1"
                     /translation="MLVLVLGASGLVGFSLVKLLLEDPFIKWVTVFVRKPLELNHSKL
                     KQVIVDFEKLNSYSEYFKVDHVFCCLGTTIKVAGSREAFSKVDLTYPIQSGELAKSVG
                     VKAFSVVSAMGANSKSKIFYNRVKGDMEEGLQKIGFESLTIFRPSLLLGDRKEIRSGE
                     KWGGRISKAFSFVFVRKLKKYKPIQAELVAKSMLENAKNKTKGFTIIESDKMNI"
     gene            complement(34291..34551)
                     /locus_tag="PLF66_02690"
     CDS             complement(34291..34551)
                     /locus_tag="PLF66_02690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62974.1"
                     /translation="MATPQSEQIQILNKMTKIIDEKVTKYKKDMKTMSTNRFYSEKKL
                     LLDTIENAIKLAESIKPPPPGVKDVIADFKKVSKDISGLRAY"
     gene            complement(34570..35025)
                     /locus_tag="PLF66_02695"
     CDS             complement(34570..35025)
                     /locus_tag="PLF66_02695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62975.1"
                     /translation="MADYPFKPLEDHFFGSIIANVWKAIGGDFDKLNLAIHSRLVGDR
                     KHYTCTSKGDKIVLMNIYPSQFRKYIQNKQKDKLFSEFEKLITSKVPNTIARLDVAMD
                     LLEWIYTGFPDDEDISKQLLLLLVNKQISFSEDFWLKLKEEYYNASHLS"
     gene            complement(35032..35343)
                     /locus_tag="PLF66_02700"
     CDS             complement(35032..35343)
                     /locus_tag="PLF66_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002975595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HMZ62976.1"
                     /translation="MAAPAKATPIKKSQIIQELSQSLDISKKDVNAFLDAFVDLAYGE
                     TKKNGAFIIPGIGKLILKKTKKRKGRNPATGQEIEIPAKTVVKFSLFKACKEAIVPSK
                     K"
     gene            complement(35513..36469)
                     /locus_tag="PLF66_02705"
     CDS             complement(35513..36469)
                     /locus_tag="PLF66_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002775763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HMZ62977.1"
                     /translation="MEAANESVEELKKKISKLEFENTNLKDELHAVQNSLYLQHTIAR
                     LNYSVLLNREEIKAKELTAGINEKQGSLFEIKNQIRKYAPILGLDPDGIRIIVTEAIQ
                     NIIEHGYGPYAEVSLEINNLVSNPYFKISFKHEMEPGKSYTLAQIEENAKKGDITSEA
                     FDWENPRGRGEFMMKELSDERQIINGVEYDENGRKSHYFKRVLINYKNPKGDRVETSF
                     DEIREEIDRLDKEEVVCYFHIDHKKSELDSITVITSSEKDASVKQIMQQAGFQLTHKD
                     NYSKTSFCTFKPNRDVTVEEIQQLFASVRKSVAAEMQHKHSD"
     gene            36545..37438
                     /locus_tag="PLF66_02710"
     CDS             36545..37438
                     /locus_tag="PLF66_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001968824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ kinase"
                     /protein_id="HMZ62978.1"
                     /translation="MNQALIVMKKTKYELDTSIYSDLDFYKKICMIQNNSFEKIYQSH
                     LRQLESRRILMEEVFPSGTFIFRDRLETLDLPKYKAIVALGGDNHFTYVAHHCDQNII
                     LGCNSDTETSLGALLAFNPLSLKATIQKDWANTTIENWSLLSVTIEYPDEKKITTVNA
                     VSEISIRNMSPDLTSRYIIKYEDKLEEQKSSGLLLYTGAGSTGWYASCKNDEDGQTSF
                     PKSANFFRAFARELSRKARQNFKLTDFTVTDSCQIISEMNGGISIDALPERIYPFPAG
                     SVATISLSKEKLKVLTHVKEV"
     gene            37478..37639
                     /locus_tag="PLF66_02715"
     CDS             37478..37639
                     /locus_tag="PLF66_02715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62979.1"
                     /translation="MMFLKVLENADELVEQYPTKAWLKKDKQKERNLSVPLFRLVNRG
                     GKMKQGVKL"
     gene            37636..38916
                     /locus_tag="PLF66_02720"
     CDS             37636..38916
                     /locus_tag="PLF66_02720"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008589142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HMZ62980.1"
                     /translation="MTQEELFERAKKVAPGGVHSPVRSFRSVGGNPIFFKRAEGAFLY
                     DTDNKEYIDFCLSFGPLILGHRDPEIEDVVRETIGLAWTFGACEPYSLELAEFIVSEI
                     PWVEKIRFVSSGTEAVMSALRVARAATGRDKILKFDGCYHGHSDALLVKSGSGLAGEA
                     SSDSAGIGQRQMQDTLVLPLDDESAVEKLFSEHGKSISCLVIEPLPANYGLLIQRKEY
                     IEKICSIARSHGALILFDEVISGFRVGMKGMADYFDIKPDLVTYGKIIGGGFPVGAYA
                     GKKELMDLVAPSGPVYQAGTLSGNPIGMRAGLAVLKKMKRDNLYADLTQRTKKFTDEL
                     KSILNSKGDNWDISLFTSIFWAHKKTEKPIRTIEEIPSGHKEGFAKLFHGMLKEGVYL
                     APSGYEVGFFSNAHTDAIITKVLEKAKKSIQALS"
     gene            complement(38888..39553)
                     /locus_tag="PLF66_02725"
     CDS             complement(38888..39553)
                     /locus_tag="PLF66_02725"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HMZ62981.1"
                     /translation="MEKFEDSFKFKSWRDTLLKHNIKIHSIRELHTVNKHNGEMLFTL
                     VHMDAVDPDGHKLLPVVLIRGHFVVVVTCLIEKESGNKYFLLVKQRRVANGDIFYEHP
                     AGMCDSEPDPWKVAVKEVKEETGLEITKENLRLLHPEPLYSSAGLLDEAGYFFACDIT
                     LSKAEIDTYRNRSAGAADENEVIITHVCTLTDVFSLIRNSNGLLATHLYVNDIKKELG
                     SIS"
     gene            complement(39603..40412)
                     /locus_tag="PLF66_02730"
     CDS             complement(39603..40412)
                     /locus_tag="PLF66_02730"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HMZ62982.1"
                     /translation="MIRILFIFFLISFSLWSGEIRVLTWNIQNIGSCKMLGENCSSSS
                     SEERSTLIQAEIARILKGYDLIFIQELSTSNSVSKDDINAFVNSLQAGYKWKVSKDVG
                     NTSGNTERYLVLFNPTKLTFEQESYIEKGCIIRSPYIIKFKEVPIYFATTHITAKKKA
                     IIPEMSCIETGLRDTYPKWVVLGDMNADCQYYPKSRGPITKAVFRDAKWYVHTGTDTT
                     VAKTDCAYDRIISDKNTIGRGVKVIRPISANPDLPEKRVSDHYPVEIIFEY"
     gene            40655..41548
                     /locus_tag="PLF66_02735"
     CDS             40655..41548
                     /locus_tag="PLF66_02735"
                     /inference="COORDINATES: protein motif:HMM:NF025684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4384 domain-containing protein"
                     /protein_id="HMZ62983.1"
                     /translation="MKKFIFLFIYFYYSIILFANGKLTIAVIGQEPNLRANIESTITN
                     SDFLQAVERNRIDTMLTEMKLGQQGVLKEGSYAKAGETLGASYIVLVEKNSNAFRLVH
                     TQSSRILGSWTGWNDKSSQEFLTILEREKSLQELANLKSAGKKDYKIEVTNAIGSKEN
                     GGVKIGDEIKIEFIVKSKKEKYAYVTILVYGQDGSITQLFPNKYQSDNKIETNIDFSF
                     PPPDAPKKYKLIASQPIGEDTMVIIASDSSVALEDGSPLGIYFGNTKSLKGTKGITLQ
                     LDKTGTVRYDVNRIVFSIKEK"
     gene            41781..42416
                     /locus_tag="PLF66_02740"
     CDS             41781..42416
                     /locus_tag="PLF66_02740"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016545517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HMZ62984.1"
                     /translation="MRLDNIDSLQSVLFRIKEISQLANQIETNPSKQFSEKPEFAKEL
                     DKALSDLQSDIQKPNQIAPKERVNLEPKRDEDLAELKNSKKTKSIDQIIETEAKLKGL
                     DPDLVRAVIKAESNFNPKAESPKGAMGLMQLMPGTADELGVENPFDPTQNIKGGTKYL
                     KELLDTFKNKDLAVAAYNAGPGAVRKYKGIPPYSETKNYVKKVNSYLNEYR"
     gene            42433..42774
                     /locus_tag="PLF66_02745"
     CDS             42433..42774
                     /locus_tag="PLF66_02745"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HMZ62985.1"
                     /translation="MENLHFDFEALRDKLEYDDPFIKQFLEMLKEELEISFTKLIEQS
                     KTNNWEGIKNAAHRIKGSALNACFNKLGKISASLEKMEDPTPEKIMSLIKQIEIEIAL
                     AKETIEEKINN"
     gene            complement(42856..43977)
                     /locus_tag="PLF66_02750"
     CDS             complement(42856..43977)
                     /locus_tag="PLF66_02750"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HMZ62986.1"
                     /translation="MEEYSEFEKKVYDALIDRKNNEVSTKVQRLNDLLSKPGEYSVSL
                     PDSKEILDRYYKSNEQNIVKLNRGSFMNKKIIYILGAAAVVLLGIFITVGNGDKPKDP
                     QVAAGELKAKVTFVLGDVKVKNSISEEGVKPQVGNYLTTGQLLFTGEKAAIDLEFSHG
                     SSIRIKANTEVAVKRLIETNGNITEEVSLKKGMLVANVTKQKQTDNFNIVTPTVIAGV
                     RGTRFLVVVDPNAAKDDVTRVSVLDGSVGITKHKEEVPLTAEPFEIIEGKESGQELTK
                     GGNFKRSGISDADIKIIEGRDEQEDQTSTIPTTVTAETEQSLFDKYGRLEVLSLDGGS
                     KVVGVITAMDENTFTVHTVKGFVKVDRKKVISHDAKQLK"
     gene            complement(43999..44496)
                     /locus_tag="PLF66_02755"
     CDS             complement(43999..44496)
                     /locus_tag="PLF66_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020780697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HMZ62987.1"
                     /translation="MGDIYERFNKRIFDFLYKYSNNQEVAADLMQETFLSYFRSYGES
                     NLPPEKAIMVLYTIARNNSINYSKKFSTVKENASNNVDIYQSQKTSFEKREELKDMEI
                     RLRHCLALLPEDQRAALIMKNINDMTLADIAEVMELSISTVSRLVVKATARLLQLAEE
                     NGIAP"
     gene            complement(44618..47830)
                     /locus_tag="PLF66_02760"
     CDS             complement(44618..47830)
                     /locus_tag="PLF66_02760"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: protein motif:HMM:NF016169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HMZ62988.1"
                     /translation="MNESRSNNIEYIICPNNSELYQALLHRLEEFKNRDNVQILKNNF
                     RIFTRENLTTDYIAFKLVEDNIPSLLFEVTPYRTIAREFLKKPISADDLISDLSCILL
                     IENIFKTKKESTHSKTNKRVLRQLATDIFKFWKDSLLSDSISKEKIPSNIKEEYYEVI
                     REFIKKKRPKKIELEHLYDLCRDQKNFLSEKDAKQKFGEEIILYGVSEIDPLHFNILK
                     ILSQYIKISIFLPVPILSVIHKEKDLLTKKDVKLRELLPEWHVLTYAENDKSLKLDEL
                     IQASNRININPDSLRFYESQEAYREIEFIGGEILRLMEKNKSNPDFRLTSIKLVLPTE
                     DTNYALIVNNVFDRMKIPYAFTKDIRKKKTPYIAAVVSLLKISVSDFDKDTIFSLFYN
                     PCFHPEIQESREDINPDVWNQIIARMNLSGFLDKYHKRREGLRETNLMTWESLWKRLD
                     SILVHQDEESSLEPELVEEAYQLLEISSSLLQDLIGFKEDFSSLKDFSRFFRILLDTY
                     LNPSMRNKPSTDPERLIQLNQRGQSKVYSLLSALEAIEEELESIKADNIHFTLEDFVE
                     TLLEQIDSWTEGDSRVLKNGVVVGELLDAVDPIFDFVYLVGLDDRRLGRSSTRLESLM
                     QEENIQLSRKNSSLKVKNYFAHIFHHTAQEYFFSYVSLDTIKDREFYPAVELDWIRTE
                     YGKKKYNKIPLFSYLEYRQSDLNAFDRDSFSLIDLKKKEAELNFLKAAYPNWDGNEVL
                     NQEIDLITRDEFLKQKIKSYFQKSYESTGVYKEMTQSQNMSVAGFMKYIHCPKKFFFD
                     YSIQTEEEEETEGDIDSIDSFRWHLFLKEALYNLLEDPSLSAEQLTKKILSPKRVEFG
                     EVPFGVLGKITEFDLLEYLERVTTIYREIMLKYKIFGKTVFNSKAIKANNSLKFSTLD
                     LPNKQISASADLLLLDGSNLYLTQIITAKDIDDIKKLNVGYTAYLVQNSNGKEELEKF
                     FKMKLNIFPAYLHLNRNIEPKLRIDSIEMFSDKIFDPFWAGLVNDKYPPAPYNLTKNG
                     HCKYCSVKTVCHGYHREFVPFLPDEINQIKNLSKALFKNK"
     gene            complement(47808..48347)
                     /locus_tag="PLF66_02765"
     CDS             complement(47808..48347)
                     /locus_tag="PLF66_02765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62989.1"
                     /translation="MSRAKHIVINLSGNFGQKLGEDVWKKIESIAHTTKAVLFDFTNI
                     DSISPEGLFFLKKIIYHLREFGAEIAFSGLNGNFVDQLKENIDKSLMHIFALREEAKN
                     FLESRSDITQENVSTVSALPEVRSALVKEGVFYTYCPSCNAKLRLKAKGNYACPACKT
                     KFFFNPIQEPLKYERISLE"
     gene            48471..50327
                     /locus_tag="PLF66_02770"
     CDS             48471..50327
                     /locus_tag="PLF66_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004766563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HMZ62990.1"
                     /translation="MFSRRYLIFPIDVVFMTISYITAHLVRYESLEFLEDQERFFISL
                     GIVIVSRSIIFLYSDIYRSVWAYASIHDLMEIIKVTLISSFVSTTALVLYNRFSQHSR
                     MVPILDTLLLLSLLCLRSFSWRIIRDQYITKNITSGKPTLIIGAGKSGLMLVTEIRRQ
                     PELNLKPIGFLDDDPAKLGAHIQGVPVLGPIDQIERFILSYRVEELIITLGALPGKVI
                     SNIIQICEAYKIHAKILPSIGEIFAGKKSSFNQLREIRVEDLLGRDPVNLEIESIKSY
                     LKGQVILISGAGGSIGSEICRQVSFFEPEGLILLDSAETALYHIDYELRNSFPNIRYE
                     SIVGDVKNLSRLGYIFEQYAPTVVFHCAAYKHVPLMEINPSEAVLNNVMGTKNIADIS
                     RLSGVDRFVLISTDKAVNPVNIMGASKRASELYLQHITPNSKTKFITVRFGNVLGSNG
                     SVIPRFKEQIEKGGPVTVTHPDVIRYFMTIPEASQLVLQAGSMGEKGEIFILEMGEPV
                     KILELAEEMIRLSGFQPYKDINIEFTGLRPGEKLYEELLLNLEGIKKTHHPKIKIAAS
                     NTNLNQMVFLSKLNQLFSLAKANKNAEIYDLFKEIIPEYQKHIDYISITKKN"
     gene            50336..50794
                     /locus_tag="PLF66_02775"
     CDS             50336..50794
                     /locus_tag="PLF66_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002103627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor
                     SspB"
                     /protein_id="HMZ62991.1"
                     /translation="MEQDLTETEIRTLRDFKIQLFETYWEKFPTFYLHVLPHPNLEMG
                     KRGLVGAEKESGVVLAFGSTAVRDISSQADYLYAELQFGFNWEKLIIPWDAVFRIYDK
                     GQNSITQLRILTDEIDFSNSESKTKKVEKKKETPSESKVIEVDFTARKKE"
     gene            50796..52403
                     /locus_tag="PLF66_02780"
     CDS             50796..52403
                     /locus_tag="PLF66_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020770140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HMZ62992.1"
                     /translation="MNQFKWIVAGDIDGFFGLMLDNLLQLLVLTALCSFVCGMPPEFV
                     FSVVLPGVGISLLFGNIFYALQARKLAIQENRNDVTALPYGINTISLFAFIFFVIFPV
                     FKATGDYKTAWKIGLLACFISGVIEFAGSFIAHYIRSITPRAALLSALSGIAITFISM
                     EFMIKTYKHPLVAFLPFGIILLQYFGKFKYPFHIPGGFLSIAAGTSIAWLAGYWGEPM
                     MKLDALKNSFSQAGIYFPNLSMTDLFDVFTLQNIKEYSSVILPMGLFNVIGSLQNIES
                     AEASGDKFETRSSLAMNGVGTIIGSFFGSPFPTTIYIGHPGWKALGARAGYSVLNGIF
                     MTILCLFGLMSVVQALVPIEAGMAIILWIGIIIGAQCFETSPTRHAPAIILGLFPALA
                     GWGVLMIQSVFNFANGELAKILAKENITTKYSITMQSVSPGLEFLPYSLAGILSLSQG
                     FLIISMIWASICAFIIDQKFRIASYWCLAGSVLSSTGIIHSYKLADNAILNEYSFPSN
                     MEFIVAYFLLAVLLFSASLVSGNTNEK"
     gene            52393..53235
                     /locus_tag="PLF66_02785"
     CDS             52393..53235
                     /locus_tag="PLF66_02785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62993.1"
                     /translation="MKNDLIKDNHQYKILDEELTKPIPNFRKIEEAISNISPAILSMA
                     ILEDVGFTYLIDYYLRHINIQVVKKILQNPMFSHEALIELFYCQVTAYHKAILQKKPL
                     PEMLTSYWTTLSKAEYAILFKNLVSRTSNIHIAKNLFSKVDLVYLRMMTSSGTLKSDR
                     VLNFFRKLGPDIQKLAAQDMHIYDYAFELAVANSDDDFLAFLDEYTVVFVQLRLVSSF
                     VDEIESEMKKNDGHKLPYFQILQIASHIPLDCLKISLEIFQEKGWISANENKAIQEYC
                     TNAK"
     gene            53268..54164
                     /gene="htpX"
                     /locus_tag="PLF66_02790"
     CDS             53268..54164
                     /gene="htpX"
                     /locus_tag="PLF66_02790"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017852795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HMZ62994.1"
                     /translation="MWFKRIGLFFLINILIMVTISIITSVLGVRGYISNSGLNMTALL
                     TLCLIWGMTGSLISLALSKVMAKWMMGVETIDPRGPYGHLYASVQRLSQAARIPMPEV
                     GIYNSPEVNAFATGPSQSSALVAVSTGLLERMNTTEVDGVLAHEVSHIANGDMVTMTL
                     IQGVVNAFTMFLARIISYGVTMAISRNDDDGPSVFSSGIYFIITLVLDILFSILGSLV
                     VAYFSRQREFRADAGGAKLAGRASMIAALENLQRTYEVVDDSRAPSMASLKISSHKGG
                     WFALFSTHPPLEDRIAALKNAR"
     gene            54260..54973
                     /locus_tag="PLF66_02795"
     CDS             54260..54973
                     /locus_tag="PLF66_02795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HMZ62995.1"
                     /translation="MAFHLERRARARKPRLLISFSLLILLLPVSNYIGMSMKYNFPWY
                     APLHLFSNANLPEWILLISSLLSGIGLFYIKRWGWWLFILSSFTFIVYNSFGFITIPE
                     SATKGPLIQTFIVTAALAYFLQRDIFIPYMKLYKRGWRLQKRYPICINVNINDTIYKT
                     ENISLGGILIKSNECNFGLNSEVTVYFELSKEGFLTKSGIASITPGYGFAIAFRNTDR
                     SFKERLKKALLLFEKKLDP"
     gene            complement(55098..56918)
                     /gene="htpG"
                     /locus_tag="PLF66_02800"
     CDS             complement(55098..56918)
                     /gene="htpG"
                     /locus_tag="PLF66_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016759335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HMZ62996.1"
                     /translation="MSESGKISVQTENIFPIIKKWLYSEKDIFLRELVSNATDAITKL
                     KKIALTEEFEGGTDYKISVTFDKEARTLSIEDNGIGMSEEEIKKYINQIAFSGAEEFA
                     KKYDSADNKAGIIGHFGLGFYSSFMVSSKVVIESKSYKKGETAVKWESESGTDFKLST
                     IDRQERGTKITLHLDSDSGEYLDKWKLKELIKKYCDFLSVPITVEAEEANKRKPLWSE
                     SPSSLKKEDYIEFYNYLFPFSGEPHFWVHLNVDFPFRLQGILYFPKLKHELDVSKNGI
                     KLYCNHVFVSDEANELIPQFLTVLKGTIDIPDLPLNVSRSYLQTDPLVKKISAHIIKK
                     IADRLTEDFKKDRTNFEANWDEIALFVKYGIISDEKFYDSAKSCVLFKSSNGNYTTLD
                     EYLERNKDKNEKKVYYAPEVEASSSIMELFKSSGLEAVLVDTRIDSHFIQYLEGKNPD
                     VRFQRVDAEITDQLIDKSTKAEIVDENNKTESTRIQDFFKEAIGREGLEIKVESLKTG
                     DVPAVVILPEHMRRISEMNLGMNHDKNALLKMHTLLINSSSKLVKNALRMSAGVNPEK
                     AKLIAQHIYDLALISSKVMSEADMGNFLKRSSQIMEDLTA"
     gene            complement(56915..58576)
                     /locus_tag="PLF66_02805"
     CDS             complement(56915..58576)
                     /locus_tag="PLF66_02805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ62997.1"
                     /translation="MIFFGRIVLFLLFTGSLFANPKIALNKGTILKGEPLELSIEIGG
                     SDPEVSLSKNVFSENGVTAEYIGLEDNTTIINSKITNKKIVKFRILTAKTGNLTTPKI
                     ILSINQQPFTAESIAFTVKPEKYTPRPKPMDSIFDQFFSGNFPGFQERAYANPNEDDI
                     KIGFYTSRSRVYIGETVIGSFTLYYKNLERPNFERNEQDPLEFPFFTTELLPNVSINL
                     PANASLDGVQYKIVPYNREIYALTPLRKGKYQLGGAKFSIEGNPLSYFSPMVKDSFKK
                     SVEVLDIPNPRPTNFSGEVGDYQIKLAFTAKEAAVGVPIPFSIQINGQGTGAMFKDPL
                     QNFCPQKKCKADITFLDENKVKKFTKLKTGEYGFESDSTFRYSLFPKEEGILNLGSVE
                     VVFFNPNTESYETISFSFPEIRVKPAPIFTPKEIPTVSSVNFQLWGFYFVVTGLGLFA
                     IYLLRTEILASAKKIFHRIPLDKFRTSPEIHLMDKAIGNKKGALLKVFLQEKGLNKAK
                     VEELVRIKSKAGNAKFSEIYSQSDARQKEIILNLIKDILKENKLI"
     gene            complement(58560..59309)
                     /locus_tag="PLF66_02810"
     CDS             complement(58560..59309)
                     /locus_tag="PLF66_02810"
                     /inference="COORDINATES: protein motif:HMM:NF012725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HMZ62998.1"
                     /translation="MIPLRIKKVYIYMRAFVLLILILITYHSIFAFDVDPVGKNVKEG
                     VERYKSQNYQGALESFNEAESQAKEDDRLFFNKGTGYYKLNDYKLALKYFEKSALSND
                     KDLRVKSLYNKGNTYAKLGDKKNAIRSYLDALSSNPDFLPARKNLEMLSRKQNQEDQK
                     QEQENKESSDDKNKSEQNKKQEQSKNKKENQQNQTSEQNENNQKDEKNKESNQKMSKE
                     EAERILESAKQNKINRKKMNERPQDRNDIFW"
     gene            complement(59325..59846)
                     /locus_tag="PLF66_02815"
     CDS             complement(59325..59846)
                     /locus_tag="PLF66_02815"
                     /inference="COORDINATES: protein motif:HMM:NF036774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgO family outer membrane protein"
                     /protein_id="HMZ62999.1"
                     /translation="MNRKNLSLYIIPLLFFSLQSSCSYFSNNTKNPQPNQTETNSQTP
                     MEKIAETFSKKMPQPNSRLIVLTFTTTEGKEHKLGNLFAEKLTTDLARRGNMVVLDRI
                     LFSKQLLDNKLSLSSANDLAEIRRIGELLGLNAIVTGMVTSFNNGYGLNCRVVDPKTG
                     FILAAEEAFYAGE"
     gene            complement(59863..60825)
                     /gene="epmA"
                     /locus_tag="PLF66_02820"
     CDS             complement(59863..60825)
                     /gene="epmA"
                     /locus_tag="PLF66_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010410050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P lysine aminoacylase EpmA"
                     /protein_id="HMZ63000.1"
                     /translation="MNQLLSNQLLKYRARYLFAIRSFFQENGFLEIDTPILKQVPGME
                     PYLDPMLVKSPHTDREGYLITSPEYSLKQALAIGLDKIYEIAHCFRSGEKGLLHTKEF
                     LMLEFYQVGIDEQGLIDVCISLFEYLQNHFVDFGFKKESCIRVSMNQLFMEKTGRSFS
                     TEDLIITLREKFPKALNNYESMYYDDLFFLVFLNCIEKDLPKGGPVFVYDYPAELAAL
                     ARVENGFAKRFEIYWNGVELGNAFYELTSPETQIHRFKEEQVKRLELGKEAFEMDTNF
                     LAALTSGLPECSGIAMGLDRLLMIILKQENLKFISPYFIDNKSE"
     gene            60825..61172
                     /locus_tag="PLF66_02825"
     CDS             60825..61172
                     /locus_tag="PLF66_02825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63001.1"
                     /translation="MNNRFKHFYIKQKSDTAVIIQCLFETIDESVEEEAGSIFNVLFL
                     STNFDVAISLENPVYITPNFIKLLLTLGQELHINGRQLTVLNAPESMVRYINRFHLDT
                     FIKILNDRDSSPA"
     gene            complement(61143..61556)
                     /locus_tag="PLF66_02830"
     CDS             complement(61143..61556)
                     /locus_tag="PLF66_02830"
                     /inference="COORDINATES: protein motif:HMM:NF025471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4279 domain-containing protein"
                     /protein_id="HMZ63002.1"
                     /translation="MLKNPKSCAYLVLSTSNLEPSEINDTLKIHPDFFGTVDENGTTK
                     TIWQLNSNLSPEEELESHVVALLKKIAPVRNEFKELNEKHIATLYCSIEYSDESKKAL
                     QLSSRSLTLIGNLGVNIEVTQWDNHKLSGAGISII"
     gene            complement(61631..61933)
                     /locus_tag="PLF66_02835"
     CDS             complement(61631..61933)
                     /locus_tag="PLF66_02835"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: protein motif:HMM:NF002017.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HMZ63003.1"
                     /translation="MSKFSQDEILSNIKEQCPDWSYEGLYIVREFLLKNFISSLQFVN
                     SVGFVAEGLDHHPEISIKWDKVRVQVYTHSIGGVGPLDFSLAAKIDQFYKLYKEIG"
     gene            complement(61939..63387)
                     /locus_tag="PLF66_02840"
     CDS             complement(61939..63387)
                     /locus_tag="PLF66_02840"
                     /inference="COORDINATES: protein motif:HMM:NF015316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HMZ63004.1"
                     /translation="MIIRKRIIILFLFCTVSLYADSYHNINGFFGEKAAGLGGAYTAI
                     SDDPSGAYYNPAGLTFAYDNSISLSASNITRTSKTYENVIGPGQGYDRSSQNYIPNFF
                     GIVKEVGGYKVAFSILNTLNETFNRNDQIVNPIYYPSVRSLRSYNIESYNQVQAGFSI
                     AKSITEKLSVGATLYYTHDTANLTNTGLVQFANRNFNGITGSDNRKTTGFMPVLGIMF
                     MPNNTVSLGLSVRHQFVTAGNRLTSGFAYQSNTNADGIYFTEGTHEANGGSIGSRPYF
                     GGKLNGRIPEVTEVRTGIAVFPTKKLMAAFDTIYTSGYQRGMNQNDFYFLGGSANQYT
                     IRDTDVRELRRYATLNFAFGLEYFLTDNFAIRAGLFTNNANSKDISWVRSAIEGANRD
                     LGSDAEVVAQTPNGVINYQIPALRSPTRNEYVNLKGYSLGFSWSTSRASIGLSVVKEV
                     GKGGSQLDETRPSQQMTYDSTAIYVIVSSKNN"
     gene            complement(63410..64192)
                     /locus_tag="PLF66_02845"
     CDS             complement(63410..64192)
                     /locus_tag="PLF66_02845"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HMZ63005.1"
                     /translation="MKSTIIVSLILFLFYCKSANTKNEKPDPPKQVYKELKIGRALAA
                     KFIKKYGLVQDLNQTKYINFIGGKIAELSPRQELNFKFGILNTPEVNAFACPGGYIFI
                     TKGALNLIENEAELVGVLSHEVSHVSLFHSGKYEAKEEIWIDMVASLLSPGGDMVTSV
                     MEAASKELEESMLENGRQKEFEIQADASAAVLISELGYDSSAYIGYLKKMKSANDNQV
                     FVKTHPPVDDRINAMQKFISDQRLPKGSKINVKRYKDNIPKI"
     gene            complement(64217..64684)
                     /locus_tag="PLF66_02850"
     CDS             complement(64217..64684)
                     /locus_tag="PLF66_02850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63006.1"
                     /translation="MNPKKLFILLFLSFTHSLFSNPVYVQSPIAKLLSEPNVASAGKP
                     LQAGQELQQISEQDMFLKVKSGNDTGWVMKLYVSKIPPSNKVSNAGNTDKSGPIQSRA
                     RASTFTETASARGLSESKKIRTRGNSDEYDFESLDWLDHVTLKPEDIQKFKEE"
     gene            64759..65037
                     /locus_tag="PLF66_02855"
     CDS             64759..65037
                     /locus_tag="PLF66_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008589277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63007.1"
                     /translation="MEAQEKLATEFKLLTKESENIKKRARENYLEQVSDISEKIKQFG
                     DEASVKAKQVIDKVGDYITENPQKATLIGVGIGVGIGVLVGLLARRKD"
     gene            65039..65401
                     /locus_tag="PLF66_02860"
     CDS             65039..65401
                     /locus_tag="PLF66_02860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63008.1"
                     /translation="MAKKKKPTSPPIEEKEEKKETLLSQVLLLVEHFVSYFELLFLYI
                     QKKFQQKVQYGFIITIAILYMLFLKVVGSILIILASYFALLKLFNGDHITTALTLGIG
                     IMILSFTLLAILIRKIVQ"
     gene            65402..65659
                     /locus_tag="PLF66_02865"
     CDS             65402..65659
                     /locus_tag="PLF66_02865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63009.1"
                     /translation="MKSKIPFNPFEGSEEFRSKNPKDMSLDELEILLHAKRLNLRLDV
                     ERLHKQINYTKLLFDIVRASGLGEMIEDLITPKEKPDSNQI"
     gene            complement(65650..66366)
                     /locus_tag="PLF66_02870"
     CDS             complement(65650..66366)
                     /locus_tag="PLF66_02870"
                     /inference="COORDINATES: protein motif:HMM:NF012799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HMZ63010.1"
                     /translation="MNLPQYSFEEKTAYLIGAGSGDIRHLTIEAHSILSQADLILYDM
                     YLEALNPYYPQAVWESVGKKKGFHVKKQSEINELLLAEVKTGKKVVRLKAGDVSFFAR
                     SSEEIQILKENGFACKLIMGISSPQLLSQALGESLTHRELTRSISYSSGYWDKTIQKV
                     NLPDTDAHVIFMGLGELEKTVSNLLQSGKSEETSFIAASNLGRQNQKIVKATLGSAVQ
                     AVKDANLQSPTLIAIGLNHI"
     gene            complement(66363..66902)
                     /locus_tag="PLF66_02875"
     CDS             complement(66363..66902)
                     /locus_tag="PLF66_02875"
                     /inference="COORDINATES: protein motif:HMM:TIGR01470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HMZ63011.1"
                     /translation="MYDNRRRPHLKKYLPLMMDVTNRKILLLGGGKASAEKLRTLAQL
                     NLRITAISPQFIEEFHSLDWIDFIYRKYQYGDLEGFDIVYCGINDPIEEKKILQEAKE
                     RKVLINFIDQVEDSDFISASTLIRENFTLFISTYGKSPGGAKMIREEIESKIDLSNLD
                     RQVGVKAKEREMKKKGLSV"
     gene            complement(66872..68137)
                     /locus_tag="PLF66_02880"
     CDS             complement(66872..68137)
                     /locus_tag="PLF66_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012388179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HMZ63012.1"
                     /translation="MDMLRFITAGSVDDGKSTLIGRLLYDSKQIFDDQMAAIEKSSER
                     RGDGYTNLALLTDGLKAEREQGITIDVAYKYFATPKRKFIIADAPGHIQYTRNMVTGA
                     STANLAIILIDSRKGVIEQTYRHTFLVALLDIPYITVCVNKLDLVEYSQEVFEKIKAD
                     YLKFASPLTEKMGIKEINFIPISALNGDNVVENSTNMPWYKGPSLIQYLESIDIDKSH
                     NYDLPRFPVQWVIRPIANEYHDYRAYAGMVNAGVFRKGDLVTALPSGAKSKIKAIDTF
                     EGEVEEAYPPMSVAIRLEDEIDVSRGDMLVKDKSPVETQEFEAYVCWMDTKKMQTGGK
                     YTLRHTTNSVKCIVKEIKYRININTLEKIENIDSLELNDIAKIVLKTAKPIFYDEYSV
                     NRGTGSCILVDEGTNQTSGACMIIEGDLI"
     gene            complement(68219..69124)
                     /gene="cysD"
                     /locus_tag="PLF66_02885"
     CDS             complement(68219..69124)
                     /gene="cysD"
                     /locus_tag="PLF66_02885"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016561004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HMZ63013.1"
                     /translation="MSKYHLNHLKELEAESIYIMREVAAQFERPVLLFSGGKDSITLV
                     RLAEKAFRPAKFPFPLLHIDTGHNFPETITFRDDLVKRIDEKLIVRYVQDSINQGKAV
                     EETGRIPSRNKLQTITLLDALAEFKFDAAFGGARRDEEKARAKERIFSFRDEFGQWEP
                     KKQRPELWNLYNGKIQQGEHIRVFPISNWTEMDVWQYILKEDLAIPSIYFTHKRDCFM
                     YDNMLLSVSDVVRPGPSDEVKEMQVRYRTVGDMTCTGAVISNASNVTEIIQEVASARV
                     TERGGRADDKRSEAAMEERKREGYF"
     gene            complement(69145..69894)
                     /locus_tag="PLF66_02890"
     CDS             complement(69145..69894)
                     /locus_tag="PLF66_02890"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002979672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HMZ63014.1"
                     /translation="MEQLEEFNQKFQISNTKEILQLALAKHKGGVLFSSSLGAEDQVL
                     TDLILSIDKTITIVTLDTGRLPYETYKTIEDTEKKYGIKIQVQFPDYISVEKMVKEKG
                     INLFYESIENRKECCSVRKLEPLSRAMKDKSLWITGLRKEQSVTRSDMKFAEWDEKYS
                     VMKINPLLNWTEKQVWDYIRANNVPYNELHDKNYPSIGCAPCTRAIEEGEDSRAGRWW
                     WEDPATKECGLHWKDGKLVPNKPKSDKSMFV"
     gene            complement(69916..71514)
                     /locus_tag="PLF66_02895"
     CDS             complement(69916..71514)
                     /locus_tag="PLF66_02895"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: protein motif:HMM:NF013259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HMZ63015.1"
                     /translation="MAKEESKITNEERIKKEKHPLKLRPEIDKWAKEGFQAIPEDDHI
                     RFKWFGCYLQKPKTDGYFMMRMRIPSGQYTASQGREIARLTKERARNLIDVTTRQCFQ
                     VHWLTIADIPVMIDTLEKIGLGVLGACGDITRNVTGNPLAGIDPEEYLDATNLINRYV
                     KEIAERPDLGDLPRKFKVSISGAPHNLAQPEINCMGIIGAKLEVNGETKYGYTIRVGG
                     GLSTNPYFSKWLNVFLKDDEEVLKVCAGLTELYRDHGNRENRRKSRMKFLMEDWGPEK
                     MRAELEKKLGYKLTDYPNPQILERNSDPLGPQKMKQLGLNFIGVPIKVGRLSGDQLIT
                     LCDIAEKYGNGLLRNTNKQNILILGVPDEKVKDAVKALEDSGIYSPLQTHSRIVSCTG
                     SEFCNLAIVETKHRTSKLIDYLNEKKINVDNINIHFSGCTNSCSQYSIADIGFIGGKT
                     KGENDQMEEAYMLHLGGSIGNNPSFTRSVLKVKADHCGPIVEKLANHYYAKKEAGQSF
                     RDFYKSYEKEQVAELLGITLAKSE"
     gene            71852..72964
                     /locus_tag="PLF66_02900"
     CDS             71852..72964
                     /locus_tag="PLF66_02900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63016.1"
                     /translation="MQTSSSQINFRSKISAFLLRYFSKFFFKDVKYYRDPNDSNNLTL
                     PYPCTIFINNVEERDILLVGYLSEIFNLQARLYFPMQRNVGKRNFPYPSNSKWKLFCK
                     LIILKLSIIPILLRLIGGKYASYDTFKLLKTIRRNTSFSIYEKFESFKQQISMQKTFV
                     LIGHESENGEKDIENVSGILRIFNNKNPDMSYLQITLTYDYLFSKEGIVHISHSGLFT
                     MKQDKPIASILATLRLKRTLGTTVTPGNLFAFAIYSPEVENGVSRQYLFYKMERLAHV
                     IYKEKFCNIARECLEMSYDDLFAEILYDARTNDFLKYQTDSKQYYGTNKLYYYKKNKY
                     YYTELKTENIYRYHYMQLYLFHKKFRQIWEEIEIYD"
     gene            73020..>73849
                     /locus_tag="PLF66_02905"
     CDS             73020..>73849
                     /locus_tag="PLF66_02905"
                     /inference="COORDINATES: protein motif:HMM:NF016593.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HMZ63017.1"
                     /translation="MNIPKIQLPRKATYTRLEEDIPFFELFQKIERKFENCFLLESLE
                     KESYDSRYSILGFDPQHIVYADGNSLYVDNVEYPCDNPYYALRELIPTDVLSVNYAGG
                     LVGFICYEGMNYFEPSLNLKPHKDFHAFHFGIYLDGIILDKMTGETFYFYYENNRIER
                     IMEIIQEENISEKSYKTNIEFLGYTMSEAEHKGKVLEVLEEVKAGNTFQCQLGLKAEY
                     KITGDTFPIYQELRNVNPSPHMYYLKFGEKKIIGASPELLFRLRNGEMETFPLAGSIK
                     R"
BASE COUNT        24125 a        13406 c        11717 g        24601 t
ORIGIN      
        1 aacgggagtc aggacatgta atgtattgta gtccaatcac tccattcttt tttaaaacct
       61 cattacattt cgcaaaatag gtctcgaaat aattatgacc gacagcttct agcatttcta
      121 tagaaacaat tctatcatat ttcccttgaa tatatcgata gtcgcaaagc tgtatgtcta
      181 tttgattttc tagacctttt ttatgaatca agtctttcgc atatttaaat tgctcatccg
      241 atattgtaac tgttgtaact ttacagccat atttacttgc cgcataggtg ctgaatccac
      301 cccaaccgct tccaatttcc aaaagatggt gagatgagtt tagttttatc ttctgacaaa
      361 gcgcatcata ctttaaaatc tgtgcttctt ctaacgtttc cttatttgtt ttaaaataag
      421 ccgaagaata agtcatagtt tcatctagaa acagtttata aaaatgattt cccaagtcat
      481 aatgatcaac aatgtttttc ttacttcctg tgacagtatt cgctctacta ttgtgataca
      541 atctattaaa aaagttaaag agtctaagtc ttcgacttga tttttttgtt ccagataaat
      601 tagggctatc ctctacattc aatataaacc aagaaatcac ctcggatata ttttctgttt
      661 cccaatctcc atctaaatag ctttccgcaa atccaatatc accgtagtaa acagactttc
      721 tataaaaatc atacgacttc acaagaatgt gcgcactgtt aaatgactca tattgctttg
      781 ttgtatctcc atataaaacc gctgttccat ctggatctac aatcttcatg gatcctagct
      841 tcatttttga aagagcattc tgaaacaatt ttttataaat ttttgtttgc aagcttaggg
      901 ataatgattg ttttccgaca acttcatttt caactgttct ttccaaggta aactcctttt
      961 tgcatgtgta tattttccat tttacgtata aatggaattt tctttaaaaa taataaaaac
     1021 gcctgccagt gaatcataag tattactttc agagtaacaa aaggatactt taagaatagt
     1081 aatatcatat tcttatcact catttctttt ttctttcccg tataagcaga gagaaatact
     1141 ttttgacctt ctttgtaatc atcaatatga atattcaatt tttctgttgg aggattcaac
     1201 ttaaattcaa actcagtatc tagctcaaaa aaaggagata cataaaaatg tttaatagtc
     1261 ctcaaataaa aacgatagtc tccatttggg ttcaaaacaa caaaaggttt catttctcca
     1321 aatgtattat gcacttctac taaagcacaa acaggaacat tctctttgtc atataaatag
     1381 tagaaacata ccggattaaa agtataacct agaattctca ggtgagtgat taataccact
     1441 cgcccaatat tcgcattaca gccattctcc ttggcatatt gtatgatccc tgacttaaaa
     1501 tcattctctc catattgaaa atgatctttc ttataaaatg aaaataaatt ccaactttca
     1561 ttactaaaaa gaagatattt atcttttaaa atatctatct catctaaatc cagactaaaa
     1621 gtaaaaagtc cataggaaaa tgcatgtttt ttgggtataa ttctgttatg cgcaacttta
     1681 cattcataaa tagatgaatt tatttccata tttctctacc tagcaatgtc cgtgccaagt
     1741 caagtgctga ggtaaaagca tcttcgtgaa agccatattt aaaataactt ccacagtaat
     1801 aaattaaatt gccttcattt aaagatggta aatcaacttg tgcatcaatt gagctaacat
     1861 taaaaacagg atgctcgtat tcaaccttac gaataatctt ttgtgggttt acctgatttg
     1921 gatcattgat tgaaacaaag taatttttat ttttagatac tccctgtaat gagttcatcc
     1981 aatatacaac tgatgaatcc ccattattct ccactttata attccaagaa gaccacgcta
     2041 attttttatg tggcataaca gattcatctg tatgtagagt cgcaatattt ttttgatatc
     2101 taaaactttg taagagactt ttttcctttt gtgtaggatt ttccaaaagc gctagaactg
     2161 tgtcggcatg acaggcaaac actaatttgt cgtaatactc aacgcttcca tcctgcaaaa
     2221 ttaatttaac ccgattcgct tcgcgtataa ctttctttgc tccatttttt aaaacaaatc
     2281 tatctttaaa tggctcaatg attttcttaa tataagacct tgaaccatta acgacagttc
     2341 tccactgatg ttgtgtattg agtcctaaaa agccatggtt ataaaaaaaa cgaaccaaag
     2401 tcacagctgg aaattccagc atatctcgct catcggttga ccaaacagca gaactcatag
     2461 ggattaaata tctatataaa atatcctccc ccattttctt ttctaaaata tactgtttca
     2521 atgtataatg tttatatttc ggatcttcta agatttgcgg cgactcattg ttaaatcgat
     2581 tgatttgata taataatttt aaatacttca cactaaacaa attagttcgt tgtgcaaaaa
     2641 gaccgttaag accagatcca caaaactcca aatgagtggg tagatgctgc acgctaaacg
     2701 acatagagga atttttggtt tctactgata attctctgaa taatcgtgtt aaatttggat
     2761 aggtaacctc attgtagacg ataaatcctg tatcaatcgg taatagctta ttaccctccg
     2821 cgacatctac tgtattcgta tgcccaccta tataatcatt tttatcataa tatgtgatct
     2881 caaaatcttt gtggatgaag taaccaagac ccatccctgc tacgccggtt ccgataattg
     2941 ctaatctagt caaaatttcc tctttcttgt tttagacttg aaacaacttc taaaaatgat
     3001 ttagaatgga attgttttca atagcctata tctatttttg accatatcct attttgatac
     3061 aaatttagaa gaaaaaaaca ttgagtaaat ttgaaacaaa agtttccatg caatgaaatg
     3121 agtagcgagt gcaaatacaa tttcgatctt cacagcaatc aacatgtaat cattgaggca
     3181 tccgctggaa ctggtaaaac atatacgatt gagaacataa tcccacaatt tatcgaaaat
     3241 aatatatcga tcgaaagtat agcagttctt acctttacag aaaaagcagc cgctgagctt
     3301 caagatcgaa ttcgcaaaaa actacttaat tctcttcttt cttcccaaac caattctaag
     3361 ttgttcgaga atgcgattga gtcttttcct aacgcaaaca ttggcacgat tcatcagttt
     3421 tgcagatcca tcctaaaaaa gttttcttct gaattaggtt tatcttctaa ctttacagaa
     3481 atcaaagata caacgaccga aattgaaacc tattttaaaa agttctgggg aaaaatcgaa
     3541 aaagatgatt ctgaagaatt aatacaactt ctgcaaatca tagattactc tgtcttcaaa
     3601 gacattatca taagaatagc agtcaacaca tccggtcaaa ttgtggctcc tctgaaagac
     3661 ttttttaatg aagatctctg caaaagcgtt tttgcaaaaa ttaaaaacct tattcaaaag
     3721 gttccctttg aaaagaaaat gcctgattat aaagtgagat taaaatctct tttagattta
     3781 aatgaatcac ttccgaatct tgaaatcata gttgcttacg aacaagcttt cctcaaagac
     3841 gatcataatc caaatacgaa tgtgaaaaaa tttctactca aagagacttc aatcagcgag
     3901 tccgaatacg atagtcttat ggactctctt tctactcaca tttgcaactt caaacaaaaa
     3961 gaatacttac cttttctaaa tcaaatttta aatctcacaa atcaatttat atcagagtat
     4021 aaagactatc tacaatccaa agataagatt cagttagatg atctcatctt tcaaacaaga
     4081 agattgattt tagaattccc tgaaattcaa aagcaattac agaaaacata tcgatattta
     4141 attctagatg aatgccaaga tacaaatcca attcaaatcg atttaatcct agaactcttt
     4201 aaaaataaaa acgctggaat tattctagtt ggagacccaa aacaatctat ctatcgattt
     4261 agaaatgcag acttagactc ttatcaagat tctataaaaa aactgaatac tcaagattat
     4321 attcaattag atacatcctt tagaagttca gaaaggctca tacaagcttt caatttcata
     4381 ttccctcact ttgcaaattt acaaaatatt tataaaccag taaaggcaag tagatcgtta
     4441 gaccctatag ctaaagaact aaaaccacct attctatttc taggattaga tgaaaataat
     4501 attccaagaa ctacagacaa atctggcgaa aaattcagtg cagactcgct tcgagaagac
     4561 tcattgagag aaatcattca agccataaaa acgatcactc acaacgaaaa ttataaaatt
     4621 caaataaaaa acacaaatga ctatcgcacg atagaatact ctgatattgc agtcctcgct
     4681 aaaacaaatg aagacctaaa taatatttta caagaatttt ccaagcattc tatttcggct
     4741 aatatttaca aaagtgtttt cttttacagc cataagatcg tgcaggctat ttctcatttg
     4801 ctccatgcaa ttgaaaatcc caatgatact tcttcaatct ataaagctct cgcatctgat
     4861 ttatttctaa ttccagaaag aactttattc ttattatctg agagaaaaga actcaattat
     4921 ttgattccca cttcttttcc agaaatagaa gaaatataca aaaccttaag aaaggcgcat
     4981 gagtatcgtt atacgcaaga catagcttta acactgcaca atctattgac tgaaaataga
     5041 attttagaat ttatttcaat gggctttgac gggaaaagaa atttagcaaa tatataccat
     5101 ttactagaaa ttttaagcta ttctcagtta acagaaaata tgagctttgg agaaattgtc
     5161 cgcaatttta aaaaagatgt agagaaagct accgaccaag aaatcaaatt agacaatgat
     5221 aaaaaagatt tttcttttaa ctccgttcaa ctgatgactt atcatgcttc taaaggattg
     5281 gagtttcccg tctgtattct ctacaatcta gcaggtggga gcaatccgaa aaacaaaacg
     5341 actatttatc tgcgagataa gaccttaaag caaaatccaa ttggtgttga attgcaaatt
     5401 aaaaacaaac atggaaagaa atggaattct cctgcatttg atacattcga aaacaatgat
     5461 gaacaatccc ttctcgaaga aaaagataga gttttatatg ttgcatttac acgagcaaaa
     5521 gactatctaa tattgccact tcatttacct gccccaagtg attcttttcg aaatcgaatc
     5581 agttctgctt tttctaacga aaacatcaat tcccttatcc aactcaagtt agcggaaaac
     5641 ttccattatc cactcgaatc taccaaaaag gatgtaaata tatcttcgca agattcatca
     5701 acggagacta tcaataaaca aattcgtaaa attgacctaa ctccaaatga aatttatagt
     5761 aaatctggaa ttcgcctcca atcctactct tctctcgcta aacatctaga atccgaagaa
     5821 gaatcagcaa aagaaaaagt agcaagtgaa aaaaagagtt atttagaatc agaagagtct
     5881 tctcctcttg caattagctc cgaaaaaaga ggaacaacct ttggacttct ctgtcatgct
     5941 gttatggaaa acttcgacct atcattactc tccgattctc ttgcaacacg tcaggcggta
     6001 actgaaatgg taaatcagta ttatccatca actggtctaa tggaaacaaa ggattatacc
     6061 agccaagagg caattgaata ctgctactca acattaacaa aatcttatcc aatgaattta
     6121 gaaaaaacag aatttgcatt tatcaaagac tggcaacact taaaacgaga acgttccttt
     6181 tattataaat taaaatctgc taaaatggac tatttaattg gaattggaga tggccttttt
     6241 ttatggaaga ataaatacta tttgctcgat tggaaaacaa atcttatcca cacggaagat
     6301 aaaacgatag acgaagtaat ccaagaaaag gtagaacact cttatcgata tcaatacatg
     6361 atctacagca tgaatctttt ggacaatatc gcatacaaat ctttagacca agaaaaattt
     6421 tgggaagaga attttggagg aatgctcttt atttttatgc gagtgacgga agacaagaaa
     6481 ggctccctac ttgtaaaacc tagttttgga gaactacaaa aattcaaaaa agaattggag
     6541 gaattaaaat gaatccatct atactacccg gaatcattcg cggtctaggc aaagcccgta
     6601 tccaatttga taaagcatat gaaaatcgta aaatcctatc cttacataat aagcaaatct
     6661 ctattttaga aaagatcgat tttaaaaata aagatttttc aaaatcctca gtatttaagg
     6721 caagtaagct aataagcgat tctgtttttg atgaagttat attttggact ttattaaaat
     6781 tgattcattc agaagaaaat ggaggaattt gtatccccaa aaatgatctt gtatctttta
     6841 taaaaaatca aaatccaaag ttacaagttg aaatcgcatg gcaaaaatat ttagatcagt
     6901 caggcgacaa ctcactctta gcctttgaag gagaaatggt ttatctaaaa agtctgtatc
     6961 aaacagaaag ctttgtatgt gataaattaa aactcttttt cgaaacaagt tttcctgaaa
     7021 aattcaaact attcaaagac aaaaacctct ccgccgaaca aatccaaatt ataaattcta
     7081 ttttaaaaaa tggaatttca tttgtatcag gaggacctgg aacggggaaa acaacgatta
     7141 tccaatccat tttgaaagca gcaatcgaaa acaaaataaa tccagaagaa attgccttac
     7201 ttgctcctac aggcaaggca gctaaacgtt tacaagagag ttgccagact ctgatcgaag
     7261 aaaatccttc tttggaaccg agcacaattc atcgctttct taaatacagt cctaaaaatg
     7321 ggaaatttag atttaattca gaaaatccaa ttcctcataa attaatcatt ttagatgaat
     7381 cctctatgat agacattttt actctaaaag cgttattaga ggcttataca aatgaagata
     7441 agaccaaaag aattgtattc gtaggagatc ctgaccagtt gctttcagtc aatacaggtt
     7501 caatattctc tgattttatt tcattaaatc gaaatctata cacactaacg caatcttttc
     7561 gccaatctaa agaaggagag gatattaaaa catttgcaaa ctatctaaaa aatttataca
     7621 aagcaaatca tactcctcta ccagaaatta tcacaataga aaaaaatatc acaaattcaa
     7681 aaaacggagt ccgcgcttta gatctaacta aaaatgcaga aatactgaat catgctctag
     7741 agtggtataa aattcattca caaacaaaag aatccatcca gattctaacg ccctacaacg
     7801 aaacagagat tggaatcaaa caagtaaatt cttatctaga ggagaactta cctactcaat
     7861 ctaaaatccc tgtaatcgtg agacaaaact tatacaacct aggtattttt aatggagaaa
     7921 cgggacttct agaacaagca aaaactcatt ctctttttta cccagcgaac aaggaaaaaa
     7981 ttgaaatatc tgaatcaaat cttcaatact tcaattcagc ctatgcaata acggtccata
     8041 aaagtcaagg ctccgaatac gatcatgtct gcctactcct tccagcagaa ctagaagaac
     8101 cagattctct tttaaatatt cgaattatct acacagctat tactcgcgcg aaaaaatctg
     8161 taactattat aggttcgctt tctttatttc aaaaatcact tctcaacaag ggagaagaaa
     8221 gacattctag aatctcaaat attctaaaat gataatgtca cacagatgac attcagctaa
     8281 gaaagacatt taaagattac caccgataaa agatacgatg atttaaatta tatcgtttct
     8341 ttatcgttta gttatcggtg tcctcgttgg taacctttta aaaatttctg ttagaccgaa
     8401 ttcactttaa ctactcttaa aagccaaatt cttaatttct ttcaggtttt cctcacagac
     8461 cttctcgcct aacgcaataa attcctttgc ccgataaaat tcataccaag aggaatttgg
     8521 caaaatggga ctcatataaa cgtccgaact ttgaaaggaa tatttgcaaa gtctatactg
     8581 caaagaataa aaactattaa taaatatatc catgagagat tgatcttttt tattggactt
     8641 gactacgtct tctggagaag gcattgcatt tacagcaata atccgctgca ctccttcgat
     8701 cgttagtgca cttactggaa gcggatttac tactcctcca tccacataaa ctcctttagg
     8761 agtaacgaga ggattaaata aaccaggtat cgccgtgctt gccattacag catccacaac
     8821 agaaccttcc ttagaaatga cttcttgtct tgtgttgata tcacaggtaa tagttcttaa
     8881 ttccattggt aattcctgaa aggttctatg ccctaaaaat ccttccaaga atttgcgaat
     8941 attatttcca gagattaatc cttttgttgg catgatagaa aaatccatta gctttaacat
     9001 attaaaaagg gaatcaaact cttccgttgc cttctctatc tcagaagcac tgactccact
     9061 agcccataat gcgccgacta acgatccaat actagttcca gaaactaaat caactgtaat
     9121 gttttctctt tctaaaacct tcaaaacgcc aatctgtgca agtccgaagg cagcgcctcc
     9181 acctaaagct aaccccaatc gcattccgga gatttttctt gcgagttttc gagcaatgga
     9241 atttagctct gaacttttta tagattcttt taaaataaca tttgagataa aatttacatc
     9301 attcgtattt tccaattcat actgttttag aaaaactgat ttatctttta attcaggttc
     9361 tgtaattaaa aaacaatgat ccgattcttg gagtaaaact ttgcacaatt ctggcttgtc
     9421 ttccggtaaa tctagaatga taaaatggta agaatatcta tacttgtgat attgatttaa
     9481 tatggcatca atatcatcaa agtcataatt cttcacttta gaagaggcat gaattaaatg
     9541 ttccttcttt ttattaaaac gtaaaacgat cgattttttt cctgtctcat taataatatg
     9601 ttccgaaagt tctaaagcga agtgtgcacc aagtgatgga tttttatcgc agtgaacagc
     9661 aattacattg gattcaatga tctttcgatc tgattcatcg ggtgatgctt ttagacgtct
     9721 agcaagaact ctactaatca ctacgcctaa agcagggacc cgattcatta catcattaaa
     9781 gtcctcctga gaaagtaaaa gtaacttaac atcccctaaa gcctttgcag aaaatgtgtg
     9841 tggtttccca cccatcatag aaacaactcc aaggaaatct ccctttcgca taacttctac
     9901 agaaacttgt ttttcctcat tgtctactct aacatgattt acaagttcta tactgccatt
     9961 tactaaaata tagaagtaat caaaaggaga tccctgttta tagagatatt catttctctt
    10021 taaatctttc aaaatagctt tttctgctat ataacttttg tcttcatcgg aaagagaata
    10081 caaaactgga agggaagaaa gtaaataata tttaccaaga gtctttaaat ctgccatact
    10141 tccaaattag aaaagaatct aaattgggca aactacaata attgaaaaaa tcaaaaaaac
    10201 attgaaagaa aacagaatcc aatcgattga atcaggtaga atccacagaa aaatcacacc
    10261 tattgaagaa ataaaaacag gaaataattc tgtatcaaaa aggctgtttc gaaagcacac
    10321 aaattgaatc tagaagacaa tccaataccg aaaccaattt agatttccac aggaagcttt
    10381 gatccattca cgccagatta aactcagccg ctagttgaaa tgccattttc aaattccaaa
    10441 tcaattttta aatgaaacca aatatcgttt aacatacgat gagttttgta aaaaatattc
    10501 tgtttccatt tatcaaatag gaaacaattt gattttaaaa tactatttaa actaaatgga
    10561 aaaaagaatt ggatttttaa atggaaacaa aagagattac agatgctgcc tccattttca
    10621 aaatggcaga aggactattc aagcaattcc ctgtatatac aattgtgcaa ggaaagcctc
    10681 aacaagtaaa ggttatgggt atgaagaatc aagcgattgt gattcaaacc ccagatgaaa
    10741 atccaaatcc cacacaacgt ttgctgatta tgacaaacgc tggaaactta cttcaattca
    10801 gttttaaagt aaatacaaaa gatccacgcg gtctagaagt attaaatcca aatactctaa
    10861 ccattaagcc agccagtaga gtatccaatc gctatcaagc aaacaaagcc cctattctca
    10921 ttacaaatat tattagtcaa actatgattc cgcatgatct tgcagatgat acaatgaaag
    10981 tggaaagttt gatgaagcca tatatccaga aactaaaagg caaatattct gaagtagaat
    11041 tctttgtgca tgaaaggctg gatccaagaa caagactatt aacagaaacc ggaaaacaca
    11101 tatttgttcc tgataccaaa aatccagaaa gtgtatctga tcaattcatt ccgtatagtg
    11161 aacatgtcca cctcatgcgt caagggaaag gtctgagcaa atttatttct gaaatctgtg
    11221 tccctcttaa atacagggga atctttatct atggatattt acaagttcta cacttagaaa
    11281 aggctgatat gaacgactat aacctgatcg ttgcagctgc agaaagattc tgtaaagata
    11341 ttgaaaatgc aggaatcttc aatgaatcca aattgaagtc ttctatttta gatatcagtg
    11401 gaaatggatt cagcttctca caccctcagt ctaagcattt tggtaaaata ttctcaattg
    11461 gtggaaccat tctattcgat atgtatgagg acgaaaagag cttgggaact tttagagccg
    11521 ttgttcgcaa tatcaaacca ctagaaaaac tatttagaat cggttgtcaa ttcttccaca
    11581 tttcagatga acacaaaatc attgatgatc tcatgcagaa attcttccct caaagtttgg
    11641 aatctcaatc acaagaagcg gataacgctc ctaagacaga aaaacctgca acagatccaa
    11701 agctaccggc ggtaaaccaa ccagtagcaa cagaaactac agaagaaagt gcttctacag
    11761 agactaacta aaaagaagca ttcaactgaa aaaagaacat attagctttc gaagcagccc
    11821 cgctttgtcc taataacact gcattgtttt gagagattga accagtatga gtattataag
    11881 agtaggctgc atttttttga tttcgaattg ctttcccagc agtcaacatc cctcctccaa
    11941 tccacaagga tacatgatca tttaaattat acatccaagt aaaatccaat tcgtcatata
    12001 gtctacgact agattttgca aatgcgagtg tcgtttgacc attattaaat tcagttgttg
    12061 ctcctgtgtt tgctgttcca ttgatcgcat accatgcatc ctgtgtctta tctttatcat
    12121 tccaaaaata agatgccata aaagttcccc acttatccgt tttataagaa agatttaatg
    12181 ttttgctgat tagatttttg gcatcaatgt tttcagacaa accagtcacg ttattccaat
    12241 aaggaatcac tccaaatctc ggatttggaa gtgtttgaaa ggtagcatta cttccatctg
    12301 ttcgattgct gtcgccagag gcaaacaagt attgtcctcc aatcctaaat ttctttgcga
    12361 aagtataacc cgtttgaaat acaaaaaatc ttcccgcata cttttggttt tgagcaagcc
    12421 aattgtaact aggagtcgtt gttccaagtg ttgttgcata gatagaattg atagcagagt
    12481 ttaaagatgg atttttattt actctagctc ctgtcgttcc agattgaatt gctccctcaa
    12541 aggtccaatc ccaaccaccc caagggcttt catccgctaa atttccattt gccgttcgat
    12601 tcgaaatcct gaatccagtc gtccataaat tatttttttg tttagaccaa gaattattat
    12661 ataagtctga atctgcagta gctggtattg taagagatgg atttgtaaga attgctccgt
    12721 taaaagaaga agttttttgt gttaaaattc caagagaata aagatccact aaaaataagt
    12781 ttttaaactt taccgtatca tacgttccaa ccaagtattg atttggttga gctggagtga
    12841 ttacagatga tttgccgcta acaggatcag tataggagac agatgatcca gatgtaaaaa
    12901 ctccattcgg tccactttga cttgctccca atttataggc gagaaagtga atattaaacg
    12961 aatcctgatc aaacataagt cttgctccgt cataggaaag tccattgatt gtccaattag
    13021 ctccacccaa taatctttga tctccataaa caagtatttg tcttcctact tggagtttag
    13081 cgtctaacgg caatttcttt gccataaacc aagcctctcg aattgtagtc tgaattggaa
    13141 tcgtctgccc ctgtccaggt gtaattgtat ttccaactcc tgcaaaggtt ggtccccgat
    13201 tgtcacccgg aacagcagcg gttgatcctc ccaagaccct tgcatcttga acagtaattt
    13261 tcattgtaaa ataagggctt ggatccatta ggaaaaacaa ttgagttgtt tgcattgctc
    13321 ttgagatata acccttgttt tgtttatcaa aaccaagatt atatctgtcc tcatatctag
    13381 gtcgaacata ggctccaact cttacccagt cattgagcca aggcttttga tctttgttga
    13441 atttttttgc tacatctgga tctaaaaaca tagaacgatt gtattcaccc gtcagtcctt
    13501 ttgtcttgag cggagaaaca taattatcct ctgagttttg atttaaactt tcggacttta
    13561 ctccatattg cttttttaat tcttctaatt cttgttgctg tttttttaaa ttggcttcaa
    13621 tttcttgcag tctttgttta tccttttcca aatcttttgt ttctgttgtt ttggaatctt
    13681 ttgtttctac ttccttagta tccttggttt ctattgtctt tgcatccttt gaatccgtgt
    13741 ttgtttgact ccaaagattt gaaaatgaaa aactgaaaag taaaataaat aaatatatga
    13801 tcttcataat ttctcctata atggctttta aatttctatt gagtgaatct gattgtaatg
    13861 cgttagcgat caagccggtg tcaataaatt gctatttcaa atatattcat ctaactggtg
    13921 tgatgaattt attgacagta ggcgcgagcg cgggtctcct aagagacaac gcccatatcg
    13981 atcggtggag gtagattgat aagatgggat tgtatttgaa attggtaaat aatatttctt
    14041 ttatgacatt gaatattgtc attgaacgaa atcaatatct taatggctgc aaagaatata
    14101 caatgcgtta gtagattttc ctcttcttct aattctgcaa aataattttc tcgaaatgct
    14161 tcagctgtat tggaataata tttttgatca gtaaacgagt tagagtgagg taagtattct
    14221 gattctgaga ttggattttc tagaaataaa aaaaaggcaa agccaaaaaa caatattcga
    14281 aaaagtaatc ttattaggaa acagtcagac atattttatc acttcacttt taaaactgga
    14341 aatgccttgt ctagttaatt tatatttatc ttactctaaa aaccttcctg aaatcttaga
    14401 aacaaaaaca agtaaataca agaaataaaa tctagtattt gcaacagtct tttctcgttg
    14461 acgcaatttt ccgattagaa gataagtgtt catgatgaaa atacttctga gtctattttt
    14521 gttttttatt acaagctatc tgtttgcaat agaaatcatt ccatataaaa agatagatga
    14581 aactttgaaa accacttggg acaatacata tccagttgag tttaaaaaag taatcaaaaa
    14641 agatttgctt ggcaaaggga ttatggttac aaaaaataag caaaaacaat tggaatacgt
    14701 ctactcattt cttgttttta tacccagagt aagtatggtc gaaggagtaa aaacagaaat
    14761 ggaagatgga aaagaaatca ttgtaaaatt agttcacaat ccgagcgata cagaaaaacc
    14821 gtatcgaatc gaactcggag aattttcaga aaaatactat caaggaaatg tagtcaaatg
    14881 gatcaaatga aacatgaaat tttaatcaaa gaaaaaatga aagcagaagg tttgagtgat
    14941 ttactcattc aagatttttt aactaaggta gaaaaagtta gaactggaga aactggaaaa
    15001 gtcaaatggg aaacgatagg cgaccttgat ccagaaaaag atgaaattga tttagaagtt
    15061 cttcgcaaaa aatacccaat taaaaaagaa actctagcta aattagtcgt gattaagtta
    15121 aatggtggac ttggaacttc tatggggctt gagaaagcaa agtctttgat tccaatcaaa
    15181 gatggaatgt cttttctaaa aattatagca aaccaagtgc agtatatacg ctccaagttt
    15241 ggagtcgaaa ttccactcat tctaatgaat tcttacaata cagaatctga ttcacttgaa
    15301 gaactaaaaa aagctggatt caaacaagac atcactactt cctttttgca aaataaagtc
    15361 ccccgtctca acaagtctga cctaacaccg attaccctct ctgacaaaaa agaggaatgg
    15421 tgcccgcctg gacatggaga tatttatctt tccttaaaac aaactgggat tttagatcaa
    15481 ctgctaagcc atggaatcga gtatgccttt atttccaatg gcgacaattt aggagcaaca
    15541 attgaacctt ccatcctaga atatctagtt gatcaaaatt tagaattcgc aatggagatg
    15601 actcctaaga cgttagctga tacaaaaggg ggagccattt atcgaaaaat gattaatgga
    15661 aagtttgtag gattagaact actagaaact gcccaagttc caaaagagca tgaacatgaa
    15721 ttctcaggca tgggaaaatt tagaaccttt tctacaaaca atctttgggt gaatttaaaa
    15781 gcattatctg cgctcatgaa agaaaaaact ccttctctat cactcatagt aaatccaaaa
    15841 gttgtagatg gaaaagatgt attgcagttg gaaacggcaa tgggctctgc tattggaagc
    15901 tttcaaaaaa caaaaggaat cattattcca agagatcgat ttgctcctgt taaaaaatgt
    15961 gaggattatt tgattcgtag atcagatgcc tatgttttaa atgaagatta ttctctgacg
    16021 atgaatccag aaagaaagaa agcgggctta agtgaaaacc tagtgagtct agatgataat
    16081 ttttataaga aaatcaaatc ttttgaaaaa ctctttccag cctacccttc tcttgtttat
    16141 tctaactctt tgaaagtaga aggcgagatc gaatttgatg cgcctatcgt cctcaaagga
    16201 gatgttactt ttaaaaacaa aagtggtagc ctaaagaaaa tttcctctct cggtagaaaa
    16261 gaatttaaag atgaggttgt ggagctatag gatatttttt aaattaaaat aaaacctcac
    16321 aatatttctg ctgaagtttt attttaattt attggctact cctcaaaaaa ctttttcctt
    16381 tttctaaaat tcattttaat tcgatacctg tcccttgaaa atgatgcttt tcaaacaaat
    16441 ttattccaga attttccatt tggaacaagt ccttgttcat cttttgaaaa ttcttttttg
    16501 attcggttca agtaatacat cttaaccttt cccttattct tggcttcaac ttccccacga
    16561 tagtcgcaat cgaaaatttc ttttacaagt tcataagtcg tataactaat attaatcttt
    16621 cccgcagttc cactggactc cattcgacta gctgtgttga ctgtatcacc ccagatatcg
    16681 taggcaaatt tcttttcccc tacaacacca gccatcactg gtcctgagtg aatacccaga
    16741 cgtaactccc agtaaggagc gtttatttct tgttttaaag acttcattcg attcataaaa
    16801 gattgtattt ccagagcggc gagacaagaa tctatcgcat gcgttgtgtt tacctttggt
    16861 atgccgccag cgcacatata agaatcacct attgttttta atttttctaa attgtatctt
    16921 tctgttactt tgtcgaattg agtaaagcaa gcatctaatt ctttaataag ttctcgtggg
    16981 gtgagtcctt cggctatttg tgtgaaacct ttaaaatctg taaacataat actagagctt
    17041 tcaaaaagca ctggggtaac aaatcctttt tctttaagct cgtccgctac ttcttctggg
    17101 aggatattca agagtagttt gtcagatttt tgcagggcaa ggtttaactg atttgttcgt
    17161 tcgattacct ttctttctaa gtttgcagat aagtcctcta cttgcgagaa tgcacgagca
    17221 aagcgagtag ataacaagaa tgcttgtgag aaaataaata taaaaaagcc gaagggtgaa
    17281 tataaatcat tttgaataat tcgttgtcca actagtatat cgtttactgt gcatgttccg
    17341 aatatcacaa atcccattaa ataagatttt gctccctctc ttttatgata aatggcgaaa
    17401 ctgagtaagt agattatgta gattaacccc acgagtgttg ttatttggaa aagtattagt
    17461 ccgtaatcta tgatttttat ggaaaaaaga aaagtgggaa ttgttaaaaa taatggaagc
    17521 ctttgtatta ctattgaaat tttttcaatt ttctcactgg agaatatttg atcaaagaaa
    17581 agtatagcca ctgaaacacc aatgtagact gttatgtatt ctatttttgt tccaatttta
    17641 aagtttaaat ttggaaatag gttgtaggca tagaattctc ctgaaaataa aatataaaaa
    17701 gatacagcca aactaagtat cccgaatgaa aggacagatt tctcttttct tcttaaagag
    17761 taaagaccga agtggtaaag acccataatg agaaagctac ccgaaagaat aaaatctcgg
    17821 aatagatact tctctcttct atcttttata ctttcgcttt cgccaagttc tatctttgac
    17881 cagagtccgc caagtctatg gtcaaaattg gaaatatgga aaacgatttc gtattcagtt
    17941 gatggatttg taatttgtat tatacgaacg ttttgatacg gataatattc ttcctttgtt
    18001 tttccaatat tcccatcttc agctataagt aatccattta tgaaaacttt gtaggcagaa
    18061 gcaatcgtgg gtattctcat agctagaggg acaggtccgt ttaccaataa tttcaatcga
    18121 tatgttgcat agccatcacc gctaagttct tttccattta ttatataacc attccatgca
    18181 ctaggaactt taatgagatt tgattttaga atatttattt tatttctatt tggttgtctt
    18241 ataattattt gattttcgac aggtatatta aaaacacttg gctccaaaaa ttcttcccaa
    18301 aaaaattccc attcgccatc gagcgaaact atcccatcgt tttcaaaatt ccaatttcgt
    18361 aggtctaata tacccttttc tgctatcggt tgattttttt tatttactgt atctttgcat
    18421 gtataaaacg ttagaagata taataaaagc aaaattatat ttcttataaa gttcttcacg
    18481 gattcccgat tactaaaata tcacgatcgt gggtatgtgc gattatccac tgcatggatt
    18541 catcgaagag atatgcatcg agttcttccc aaggctccag tgaccgaata atttttagaa
    18601 actcggaata cttgattgca taaaatttat ccaatcgttc attaaagaaa aaaagacttt
    18661 ggctaggctt aatcgtgata tttttaggga gaacggctaa ttcgtgtttg ctttttgaaa
    18721 ccttaggttt ttggttaaag gaattccaaa tcaaataatt atgaacaagt acatctttat
    18781 tcgcgaatct ttctaagatt gcctgtttga gtttattttt cttgataccg gaaattgctc
    18841 ttgtatttgg cgctaccatc attattcctc ctcataaaaa atatgacctt ctgagccatc
    18901 tgatttacca ttggaccaat ccttccactt gatatgagga agatcatttc ctacgactgg
    18961 ttctgctgga tgaaattcat aaccagattt atatggctct ttcgcataca ttggatattc
    19021 tgccaaatct ggttttgctt ctaaaattaa ttctttcgca gtttctttat ttttaacagt
    19081 agcattattt ccttttcctt tactatcttt aattcgtttt actgcatctt tccattcttt
    19141 agaatttttc ttaattattt ttctagaagt cttctttgga atgttttgtt ttttggcatc
    19201 gtaaatatct ataacactat tagtaaaata gaccgttagc cccaaagtga ataaaatcgt
    19261 tgcaataacc caaacaaatt gggttttctt ttttcttggt ttagtttgta gattagaggc
    19321 aaaatgattc acaacaccat gaaataatta taaatatttt gggtaaacta caaatatgta
    19381 gatttttatt ttgaactaat tcactctgat catatatata cattaaaaat tcgtaaaaat
    19441 caatcaattc agcgatggaa gtgattacaa atcaagaatg ttagaattta cattataagt
    19501 tttggagcct ttactaaaaa gattcttctt tttgtaagca tttcaaccct tttattctat
    19561 aaaattaaca ttgcatcgcc gtaagagaaa aagcgcatct tttcttttat ggcagtgtga
    19621 taggcttcga gtatcaaatc ccgacctgca aaagcagaaa ccaaaagaag taaactactt
    19681 tctggcaaat gaaaatttgt aatgagtcca ttgatagatt ctactaaatc tcctggctga
    19741 ataaaaatgt cagtaatccc ttgttttcga agaaagctct tggaagattt atcataagaa
    19801 gactctaaag ttctaagaga tgtggtgcct atcgctataa ttcgctttga atgatccttc
    19861 ttagcttcat ttaaaaaatg aaacgatttt tcagggatgt aaaactcttc ttcatgcagt
    19921 tttttttctt ggatttgctt ttctgtcaga ggagaaaaag ttccataccc aatacaaagt
    19981 tccacttcgg taaaggttgc tccctttttt tgaatctcag attttaattc atctgtaaaa
    20041 tgaagtcctg ctgtaggtgc ggcaacagat ccacttgact tagcgaatat cgtttgatac
    20101 cgttcattgt cttttgcatc tgcttttctt tttagataag gaggaatcgg gatagttcct
    20161 attttttcga atatgtcttc tgtaatttct attgcagaga atagatatat atctccttca
    20221 gaatttcttt ctaatcgaaa agaaatggat ggatctatcg aaactagtgt atcgccttgc
    20281 tttaatttcc tgacattttt aactaaacat ttccaagttt ttttatctga atttttattc
    20341 tctagaaata tagtttcaaa ctctctacca gaatcagatc gcaaataaac tcttcgctta
    20401 ctcactctag tttgattgta aacaagtaaa tctttactat ctaaataatt gataatattc
    20461 ttgaacatag gttcaatttg aatcgaattt gtattacgat tcacaatcat aagcctagac
    20521 tcgtctctta catctaccgg ataacgtgca attaactctt cgggtaaatc aaatttatat
    20581 tctgttaaat caatcatact agcttatttt tctatctttt tagacaagac acaaagcaag
    20641 gttggaaata ttcttagatt tgtcatttcc ttttttcgca actaacgaga ctacttaaaa
    20701 gcaagatatt catcacgaag tgcacgaagg ccacgatgaa aaaaagtaaa tttcctagga
    20761 aattttgacc ttcgcgatct tcgtgctctt cgtggttaga tttttttaag cttttacaca
    20821 ataccgttag ttatttggat gcctggcggc agcatagata tgtttatctt tgcattaggt
    20881 aagttcataa tttaaaccaa tattaaataa cactagttcg atgttacaaa aaatttaaaa
    20941 gattactgcg acccttagga agaccaatat tgagtttgga cactgatgaa catgaataaa
    21001 ataataacta ttaaaaccat atgcgcttat ctgtgttcat ccgtgtctat cctgcccttc
    21061 gctccaagta ttcgcgacct acgcctttgc atgtggttaa atattcttat atcgaattca
    21121 cgttaaatat tgatttttta agaccatatt tcatattgag aaagaaaata tgttagaaag
    21181 aatcaaaaaa ttctcacctt atctgctagg aattatcttg ttggttttac ttgtgcaaag
    21241 tatagggcgt gcagataaca aatctgattt tcatgattac tatactgctt ctcgtctttt
    21301 ttatgaccaa aaggatttat acaatatcaa tgctttagaa agtcttagag aggaagtaaa
    21361 actagaagac ctttttaaag cagaaaattt aaaaaaatta gaagctctaa aaggcaatct
    21421 aggaacctat atttaccctc ctctttttgc ctttctactg attcctcttg gttttctaaa
    21481 ttacgaacta gcagcaacta tttttactct gattaatttt agctgtttac tcctttctct
    21541 ttttttaatc acgaaactaa tcccaattaa aaatattttt agtattcttt tttttacgat
    21601 tgcagtgaat taccgttatc tggaaagcca tgtgtcaaat aatcaagttg cgtttatact
    21661 gattcttttg atccttcttt ctttactcat aaaaaatgac gcgttgtctg gaatactcct
    21721 ctctcttgcg atcatcataa aaatcacacc tgccattttt ttattttatt ttttatacaa
    21781 aagacaattt gcccgttttt tctatacctt tttatttgcg ggactttggc ttctacttcc
    21841 ctctctctat tctcatgaat acaatttaca ctctttacaa aactggtatg atctagtctt
    21901 aaacaacgcg atgaaaaatc caacttttcg atcttggaaa aacaatcaaa gtttgattgc
    21961 cactctcgca aaatttttta tggtgggtgc agatccactc aatcaagcac tctttggaat
    22021 gccttatcta aatttgagcg caagaactct tacttattta ttttattttt gttcactcgt
    22081 agtcggaact ccttttttat ataaattaaa agaaggcttt aaagatgcaa atttgatttc
    22141 aatcttattt atcttgtctg ttatttttag tggaattagt tgggttcatt catttgtctt
    22201 tttattattt ccgattgcct atctatctca gcagtttttc gaggagaata gaaatgaaag
    22261 caaacaaaaa atattttatg ctctttgcgg tataacaata ttaacctctc gaggaataat
    22321 tggttctact ttggaaggag tatttttaat gttttcaatc ctactttata tatcgattgg
    22381 attttattta cttctcatca aaattaaaac tgatgataca accaatgaat ttaaggttta
    22441 aatctctatg caattagatg taaaatacaa acctagagtc gcggtagatg caagaccact
    22501 cgcttacggg cttacgggaa actcacgcta tctatttgaa gctttgaaat tactcattcg
    22561 aaaagactct cattttgaat actatctcta ttcaaataaa gaaattcatc caatgtttat
    22621 ggattttata aaacaaaatg aagtctatgt tgcaccgatt aaaaatgtta caggtgttct
    22681 ttggctgaat tttattttgc cttatatgat gtataacgat cgaatcgata tattttgggg
    22741 cactctacaa cttctcccat ttatcaaact cagaattcca gaatttgtaa attaccacga
    22801 tctaaatttc agatccgcac ccgaaaccat gacaaaagca aattatattc agcataaact
    22861 tttatctcct agaagtttac gcaatgctga tctagtcttt tgtctttctc aaaatacaca
    22921 aagagaaatc gctgaattca aaccagaata cacaaagaag ctaaaagtga tatatcctgg
    22981 ggtaagtaag aaagtcatta caaataacac acttcctttc agcggcaaat acctatttac
    23041 cattggaaca ctagagccaa gaaaaaatat cgcaactgta atcgaagcct atttactcat
    23101 caaaaaagat tttccagatt ttccctacaa acttgtcatt gcaagtcgtc gtggttgggg
    23161 acaacaggaa ttaacgcaaa aactaatttc gggtgaatta gaaaaggatg gagttgtgtt
    23221 tgtagaaagt ccaagtgatg atttactcaa cacactctac cagaaatgta gtgccttttt
    23281 atttccctct atccatgaag gatttggtct tccgctcctt gaggcgcttc tagaacaaaa
    23341 ggtttgtatc gcatcagaca ttccagtctt tagagaaatt ttagacttag actctgatat
    23401 actcgcaaac cctagagtta ctgaagaatg gaaaaatgcg attttaaaaa taaccgaaca
    23461 agatcctatt aaaagaaagc gaaagtggaa tgaaaaacag tggacatggt tagccactgc
    23521 aagccaaata gaagaatcct ttttaattga atggcataaa agattgagtt cgagcgttaa
    23581 caatgcagtt taattctata cactttatta ttttcttttt tttctccctg attctgggaa
    23641 atattttaaa aaacacatgg caaagagtat ttttattatt agcgagtgtt tatttttata
    23701 cttacacaaa tacttatttt ctagcactat tgattttatc tacactcatt gattactttg
    23761 ctgcacttgc cattgacaat ccaatacgaa cagaaaaaca aagaaaaatt ttcttaggaa
    23821 tttctgtatt ttttaaccta atcattctag cttattttaa atatgcgtat cttaccacag
    23881 atattatcaa tcaaatcttt ggttctagtc ttagaacttt taatatagtt gcctattatg
    23941 tgaatctatt gcacctaacg cctaacccac aagatgtatt cttagaaaaa atcgttctac
    24001 ctgttgggat ttctttttat acatttcagt caatgagtta tacaatcgat gtataccgca
    24061 agcaaatgga agctcgtaag tcttttattg acttctcttt gtatgtagct tttttcccac
    24121 aactagtagc aggtccgatt gtgcgagcaa ctacattctt ccgtgactta gatcatagac
    24181 tttctgttac caatgaagac attcaagttg caactactcg gattctaatt ggctttatgc
    24241 gaaaactagt ttttgcagac aacttaggca aagtagtaaa tcatacattc tttcattatc
    24301 aaacaatgaa tgcgattgaa atttggacag gtgccattgc atttggttgg caaatctatt
    24361 ttgactttgc aggctataca gatattgcga taggcgttgc acgtctcttt ggttttaaat
    24421 ttgatccaaa ctttaatttc cctatgagca ctactagcat tacggatcat tggacgaaat
    24481 ggcacatatc gttctcaaca tggattcggg attatatcta tattcctctc ggtggttctc
    24541 gcggtggagc gtggcttaca tacagaaata tattcattac ttggcttttt ggtggagtct
    24601 ggcatggagc cgattatcat tatatcgcct gggggctttg gcaagcggtg atgctttcca
    24661 tccatagatt ttattcacaa actaagctaa gagaattttt aaatacaagt ggtggatttt
    24721 tatacaaagc tttctcggga gtatttacac ttttctgcct tgcctttgga tttgtaatgt
    24781 ttagagcaca aaatgatcca aatggcacag caatgcatca tgtatatgag atgtgtaaat
    24841 ctatgttgct tttaacagat cattccagag tatttgacag ctactccaat tacgagtttg
    24901 ggatactgct tgttatctgc tacttggcaa gtggatattt ttcaaaacga aatattgagt
    24961 ttatggcaac gaatggaaac aaattgcttt atgcgaacgt agttgctatc tttttgattt
    25021 tactttttgg cgctgaaact caaactttta tgtattttca attctaggta gaatatgaca
    25081 gagtttaata aattttataa agacaagcga ttttacgttc ctattttaat tctaattgga
    25141 tttgaaatat tctttcagct ttttgctgat ttttacaaac cctatttaaa aaagaaatcc
    25201 tatgctgcta atattaacca aattttagga catatcatag acaaaaaaaa ggaacatgat
    25261 ccagatattt tgattatggg aacgtcagtt gcctaccaag ggctttctct tccagtcttg
    25321 caagaaaaaa tcaaagacac cggatataaa atccaatccg tcgcaatccc aggctcagaa
    25381 cttattgtgc aaagtctcgc gattgaaaaa gcattaagag aatttcaaaa tgtaaaattg
    25441 gtgatctatg taggggaaat cacaatgccc tgggtcagta aaaaagatct tacaccacct
    25501 actcttgcta tgatcaacga atttgataaa aaagattctt ttcctaaagt gatagatttc
    25561 ggttatgacg caggagaggg tctaggtttt ttactacact acgacaactg gtcctatcta
    25621 ctttttaaaa gtatcgccta tcgtcgagat ctgagtgatt ttattataga tcctgctaaa
    25681 cgattcaaat acttagcgcg taaaaaagca aatccgaacc taaacttcta tgaatatgaa
    25741 aatgatcgtt cagaaaaaat gagtgactat catatcacaa gtatagatga ttgtttacaa
    25801 aaaaccaatt ttgaatttaa tcatgaccca attccgccaa cttctaattt tgatcataaa
    25861 aaagcaattt ttgacacttg ttgggttgga aaattttcaa cgcctgcgca gactagaact
    25921 gctgaatcag atttatattt taaacggctc tcatatatct attctcacat tcgttctaag
    25981 aatattaaaa tcatcaatat atttgcacca tacagcactt ctgtcgaaaa ccaattgggt
    26041 ggagcaggaa gagtgcagat ctggaaggaa gaattagaaa aactaaatcc accaaatcca
    26101 attctaattg attttagatc tgtatttaat gggaaaaata gtgatgatta ttgctacgat
    26161 gtaattcacc taaatagaaa cggcgctatc ttattttcag aagcgttagg agattatcta
    26221 aaagataatg taaagaaaat cattcaatag gatatataaa tgctatttac ttctctactc
    26281 tttgctgcct tctttcttct agtatacatt gtatattggt ctatcccaaa tcaaaaggga
    26341 aaagaaattt ggcttttact tgcatccatc gttttctatg gttcttggag ttttggattc
    26401 ttatttcatt ttcttggaat gattgcaatc aattactact tcattcgaat tctctatgac
    26461 aaccagtcca agaaaatcat cacgcttgca gtcatcatca acttaatcaa tctcggaatc
    26521 tttaaatact tttatttttt cacagatata tttgcaagct tgagcggaat agcagcatta
    26581 gccgatctaa aaaagagcac aaccttcaaa atcattttac ctttagcaat tagcttctac
    26641 acatttcaaa tgctagctta tataatcgat gtgtatagaa aaaaagtttc cgagaaaatt
    26701 tctctctttg attttacaat cttcatctta ttttttcctc agctcattgc aggtccaatc
    26761 atgcgttcgc aagatttttt acctgaactt agaaatatca aagccaaacg agaataccta
    26821 acgcccggac ttagttttct tgcacttggc atattaaaaa aagttttact cgctgacaac
    26881 ctagcccttc tcatagaccc tgtttggtca aatccagcaa agtatgaatg gactacactt
    26941 ctactcgcag cgcacgcatt tagctggcag atctattgtg actttagtgg atatacggat
    27001 attgcgcgag gctgtgcatt cctacttggt tttaatatcc cagaaaactt tcgctcccct
    27061 ttcctggcag actctgcaag agacttatgg cagagatggc atataacgct ttctacatgg
    27121 cttagagatt atctctatat cccactagga ggaaatagac agggagaatt tagatcttat
    27181 atcaatttaa ttttaacttt cactctaggt ggtctttggc atggagctaa ttttacttat
    27241 atcatttggg gattttacca cggtgtaatt ttagcaatag aaagagcatg tgaaaggttc
    27301 aatattcgta taacgtcaac aaactttctc tggaaacttg tttgtattct ttatgcttac
    27361 catatttttg caatcggggc tgctatgttt agagccaata gtgtggcaga ttttataact
    27421 atgttttctg gaatattcca aggtgcatct ggtattgcaa tgagtaaaac cgattttgaa
    27481 acggttcttt atctttgtct atttagttta ttcattcaaa tcgttcaata ctttgagaaa
    27541 cttccaatct gggttgtaaa atctagacag gttttaattc ctctgtctta tgtaatttta
    27601 ctttatttga ttagccttta tagcaaatca ggaaaagaat ttgtatactt tcaattttaa
    27661 actatgaacg aaacattttt aaaacataaa tttctaatct atcccctcct cttagcaagt
    27721 tttatttttt gctttgataa attgtttggg actgactttg ttcgcaaata cactgagtca
    27781 agaattgagt atgcattcta tgcagaaaaa ggagcgttac tcgctcaact gctcgaattt
    27841 caatccaaaa agaaaccaaa tgaaaaactt ttagttttat ttggaacatc tcattttgga
    27901 gagttttctc ataaatacat ccaagaaaaa gataagcgac taacaaccta taacttctct
    27961 gccccaatgg cacctccttc ttatctctat tataacctac acatacttct aaaagcaggg
    28021 gttaagatag attatgcggt gcttgaaatc attccggaaa tctttcaaga tgcagccaat
    28081 gagtatgcat taaaattttc ctatgactgg aaatttatgt ttgagaatcg agatattttt
    28141 tcaggtagcg aattagaaag ctacactcat gcaaatctat ttaacgtcat acgctttcct
    28201 cctagactga atattgcaat gcagagaatc aaagacaaat ctaaaataga tgcatttgaa
    28261 tactttaact caatggttca tctcgccacg aaacaaaaca atgggggaat tccaaacccg
    28321 attattcacg aagtcccaga ggaatttttc gaaaaagaat cgaatgtata ctttggacaa
    28381 gctttccgaa aacaaggaaa ccaaactttt gtagaatcaa atgtgcagag aaaattcttc
    28441 gaaaaattca tagaaacttg taaacaaaat aatattaaat tattggttta taaacctatc
    28501 gtctctaaat cacttcaaaa acttttagat gaatctgatt tttacgcaaa ttggtggaaa
    28561 gaaaaatcgg aatttgcaca aaagaatcat ataccgatgc tcaacatggt agattataca
    28621 aattctattc aatgtcaaaa atttgtagat gtgcatcatc taagcggcgg atgttatcca
    28681 gaaatcacag atatacttct cgaaaagatt ttaccacaga taaacgatga gcacagataa
    28741 gaattcaaat cctgatcaag atatcgctac gaacaatgca gatttaactc taaatcaggc
    28801 tcaggcgcaa gtagataaat ggataaaaac tatcggcgtg cgctatttta gtgaactcac
    28861 gaaccttgcc ctactcgtag aagaagtggg tgaattgtct agactcattg caagaaccta
    28921 tggagaccaa tcttttaaaa aatcagattt agaaaaagat attccttctg aaatgggaga
    28981 cgttttattt gtattgatct gccttgcaaa tcaaatggga atttctttag aagatgcatt
    29041 aaataagacg attcaaaaaa atacgtcacg cgacaaagat cgacataaag aaaataaaaa
    29101 actaatacaa taaaacaaaa gataccacca acttgcactg agccatatcg aatgtgtagg
    29161 aactattatc gccaataaaa gatattataa ttccaaatta tccatcgcat cttcatcgtt
    29221 cagttatcgg tgtgcatcgg tggtgaaaaa attgttacgg cgaattcgcg ctaatatata
    29281 cagcgaacat agttgcggta ttgcttaggt atatgaagaa tagtgccaac atttagaaca
    29341 actgcccaag ctctttctga gtcaagttca tcttttgtcc aataccaatt ctcattccat
    29401 tttttaaact ctcgtgtctt gtatagggaa agcaattcag atttagttgg aagtcgcata
    29461 ccattttctt ggcatttttt atttgcgtct gcccaagtta aactaccaaa ctcttttccc
    29521 cattggttgt ttacttctgc ggttaatggt aaaatgaaaa atgaaataaa tagaataaat
    29581 tttagaagca taaatccaac ttaatataaa aaaacaaaat tgaaactgat tagtttaaat
    29641 tttaacttgc tttacaaaat agcaagtcat ttactacgaa tatcattcat ttatagattg
    29701 ggtaggtaaa tatgtcacac aaacacgaag aaaaagaatt tgaactcaaa tccattgcta
    29761 acctactcct tctgcctgag aaagaaattt tatatctaga taaattagaa tcaaatgaaa
    29821 tcaatcgact tagacaacag attatcaatt cagtagaaga aggacaaaag caaatttgga
    29881 ttccgatggc aaaagttagt aagttcatgc caaactttct aaatgcgaaa gttacagaag
    29941 atattttagg acctagtatt gcggctaacc tctcttatca tctccccgca aaagatgcgc
    30001 ttgggatagc agcttatttt tctattcctt ttttttgtga tgtattagaa cacttagttc
    30061 cagaaaaaat tgtagatatg attaaacagt ctccctttga tttaatgcga agagcagtca
    30121 atgaattgat aaaaagaaaa aactattttt taattggaag cctaattgat tatacaccaa
    30181 ctgaatctgt agacaaaatt gcaagaggac tctctaatct aggagatctt atttctatta
    30241 cttacaatgc aagagacaag tcaagagtct taggattatt caaacaattc gatgatgcaa
    30301 agattctagg agtcatgcaa gcaggtttaa acaccgaatt agcaaacgaa ctgaaaacaa
    30361 tttacgagca tgccgatgag gaattactcg ataagactct aagaattatt aaagcgaaag
    30421 atccaaactt attagaaaaa tacaacgata tgcttagcca ttttgataaa gattaaatag
    30481 gagaattttt atgagtcaat ctacaagtaa taaaagatat acattggata gtctagctga
    30541 gttaaattca gatgaactag aatcactcta tcgcatagga actgttcctg acgattttag
    30601 atctgtccat ggaaaaccaa aaggtagaat gttaactgtt cgtccgacag acggcacacc
    30661 tatctttcat gtattaaaac aaatttctgc acacggagca tttccttggg atggaaaaag
    30721 cttttcctat tcaggaaagc aaacgggtca aggaatcaat cgtgttaaac ttcttttttc
    30781 caatttcgat tggtttccat ttgaaattag agttgaacca tctgttattg atgacaagcc
    30841 ttgtattttt ttagattatg aacagcctga aaatccttgg ttcattgcaa agattcacga
    30901 tgaaatcaga gaagtttctc ctggaattta tatgggacct gctatgtgga aagaagataa
    30961 atctagtcct atactcgttt tgtggtttgc actcgatttc aataatcaaa ctgtttagta
    31021 aaacttttta gaggattacc accgaggaca ccgataaaag atacgataat tcaaaattat
    31081 ccatcgtttc tgcatcgttc agttatcggt ggtgaaaaaa tttttacttc gaatacacgt
    31141 taaatataac gttatactct agcttctagc cttcatagaa ttcggttgtc cagtagacgc
    31201 tacaaccgat ctccaaagag agcttctttc tggaaccaat acctttctct cagaaatcgc
    31261 aagtgtaata ggtatatttg taaatacatt attccaaata cctacaacca ttcctgttct
    31321 accagccata cctgcatgaa ctgcattctg agctaggaag ttacaaaaca ccgaatcctc
    31381 tgcgttagct ggcacacttc gaatgatata acttggatct atgtatttta aattaatagg
    31441 aatattttct ttcttgaagt attttgaaat ttcattcttt aagaaaattc caatatctcc
    31501 tagcttctta tttccagatg gatcagtttc agaattgtta tcaaaaaatt tctgtcctgc
    31561 tccttctgcc acaagtatta ctgcatgatt tcgacgaatg attctttctt taagagctgg
    31621 aagaaaagct cccttccctt ctaagtcaaa gtcttgctct ggaatcaata caaagttaac
    31681 atcttttgaa gcaagtgccg catttactgt aataaaacca gaatgtcttc ccatcaattt
    31741 aactagacca attccatttg gtgcgccttt tgcttccaca tgggcacaag aaattgcagt
    31801 cattgcttct gcaaatgcag ttgaaaaacc aaatgttttg tgaatcatat tgatatcatt
    31861 atctattgtc tttggaattc cgatgattga tattttttct ccacgatttt taacttcttc
    31921 gtatatagca tttgcagcgc gtaacgtccc atccccgcca atacaaaaaa gtatattcac
    31981 tccatgaagt ccaagaaaat caaccatttc tcctatatcc tgatttcctc gagaagaagc
    32041 aagtatagac ccaccatctc taactatatt ggatacaaga tctggtttta gttcaatagg
    32101 cttatgacca aattttttaa cgagtccttc aaagccatat ttaaacccta gaatacgtgg
    32161 aacgttatat ctataatgaa gttccataac taatccgcgg ataacatcat ttagccccgg
    32221 acaaagcccc ccgcaggtca caataccagc cgtaaccttt gacggattaa agtatatttt
    32281 ttcaagaggt ccagattgct cgaaatatat cggattctca ttgaaagcat ttttcatact
    32341 ctcaggattt tcaaacgtgg attggtagat tacaacggaa ttttcttttg tataaaactc
    32401 ataaccggca ggatttggaa ttgtgcattc cccgaattta tcaactttgg tattcatagt
    32461 tatcatatag gtatctctta ataattttta gagatttctg caagtaattt gtttttattt
    32521 ttcttttttt gcaaaaaatc atttgccttt tttatgtgtc tttggaaaat tatcgaagtt
    32581 tatctttatg tactgagtct ttcacaaaat aaggtctaca cttcatgaaa aaatcctctc
    32641 taaaaatgat tgctataact agcttgcttt tattgacaga ggtattttct gaaaacttaa
    32701 aaacaaatcc tacgaataag aaatttgata gtgtagatca atctgattgg gtaatatcgc
    32761 ataattttta cgatggaagt tattactcac aagcaattgt aattcctttt aattatccat
    32821 ttgaagctag tttaatacct ggtgccacaa agcccaacaa tctaaactca attgatttta
    32881 tttctaaatt caacttctct actccaagcg ctgtaagttc tggtaaagta attttaaaac
    32941 catcagaaaa tacagagttg gctgcataca actcaaatat cgtcacggat agcaatcaaa
    33001 ttctaagaga tacaaacctg agaatgccat ctccttggca gagaaattct actgttgcaa
    33061 ataccacttc tgggtttgtt tttgcttatc tgaaaaaaaa catagggttc aatattgatt
    33121 tatccctgcg tatgattgga aatgaggcag gacaagctgt attagattct gtttcttccg
    33181 gtttaagctt ttcctatcgt atctctgatc gagtttttca aatgaatcca aactatcgat
    33241 tgaatttaat attccaagta tcttcaatgc aatacacaga tgaaagagca atgcctaaaa
    33301 aattagaatt ttctaaaaat ttgagatccc agtttatctc atccggattt actttaaata
    33361 cacgatctgt gatgtttgaa ggactcattc gattgcctgt tcaccaacaa gctatgtttg
    33421 acttagatgg tttattacga cctgaattgc aaggtagact tggaatgaaa tggtatttgc
    33481 cagaatatat ccaaccttaa taaatttaaa cgtcccatct gaatcctagc aatatttcca
    33541 tgatctgaga gacgaaaagg ttaatagata tttgcctctc gttattgcga ataaatctct
    33601 ctaaaatgtc tgtttgttca attctcgtat actaatttta acttaatagt tcttaaatat
    33661 tcatcttatc tgattcaatg atagtgaaac cttttgtttt attcttagca ttctccaaca
    33721 tagatttcgc aacaagctct gcttgaatcg gtttgtattt ctttaatttt cgaacaaata
    33781 caaaggaaaa tgcctttgat attctaccgc cccacttttc tccggatcgt atctcttttc
    33841 gatctccaag aagaagagaa ggtctaaata tcgttaaaga ctcaaaacca atcttttgaa
    33901 gtccctcttc catatcacct tttactcgat tgtaaaatat ctttgatttt gaattagcgc
    33961 ccatcgcact tacaacagaa aaagctttta caccaactga ctttgctaat tccccacttt
    34021 ggattggata ggtcaaatct acttttgaga atgcctctct tgacccagct actttaatcg
    34081 tagttcccaa acaacagaat acatgatcta ctttaaaata ttctgaatag gaatttaatt
    34141 tttcaaagtc gacaataact tgttttagtt tggaatgatt gagttctaac ggtttacgga
    34201 caaataccgt tacccatttg ataaaaggat cctcaagtaa aagctttacg agagaaaatc
    34261 ctactaaacc agatgcgcct aatactaaaa ctaatatgct cttaacccag aaatatcctt
    34321 agatactttt ttaaaatcag caattacatc tttaacgccc ggtggtggag gcttaatgga
    34381 ttctgccagt ttaatggcat tttcaatcgt atccaaaaga agttttttct ctgaataaaa
    34441 acgattggta gacattgttt tcatatcttt tttgtatttc gtcactttct catcaatgat
    34501 ctttgtcatt ttgttgagaa tttgaatctg ctctgactgt ggtgttgcca tttctcctgc
    34561 tccgagttgt tagctaaggt gagatgcatt ataatactcc tcttttaatt tcaaccaaaa
    34621 atcttcagag aaagaaattt gtttatttac gagtaaaaga agtaattgtt tggaaatgtc
    34681 ctcatcatct ggaaaccctg tataaatcca ttcaagtaaa tccatcgcga catctaaacg
    34741 agcgatggta ttcggaacct tggatgtgat caatttttca aactcagaaa ataatttatc
    34801 tttttgttta ttctgtatgt attttctaaa ctgacttggg tagatattca tgagcactat
    34861 cttgtcccct tttgaagtgc aagtatagtg cttcctatca ccgaccagac gagaatgaat
    34921 ggctaagttt aatttgtcga aatccccgcc aatcgctttc caaacatttg ctataatact
    34981 accaaagaaa tggtcttcca gcggtttaaa aggataatca gccaaattaa attacttttt
    35041 agatggaacg attgcttctt tacaagcttt gaatagagaa aattttacaa ctgtctttgc
    35101 aggaatttca atttcttgac cagttgcagg gtttcttcct tttctctttt tagttttttt
    35161 caaaatgagt tttcctatac ctggaatgat aaatgcccca ttcttttttg tctctccata
    35221 agcgagatct acaaacgcat ctaaaaatgc atttacatct tttttagaaa tatctaaact
    35281 ttgagataat tcttggatga tttgagattt tttgatcggt gttgctttag caggtgcagc
    35341 catattcttt tgtccttata aacctttttt taagacataa aagtctttgt tttgctaata
    35401 caatcgggct atacagattt ttttttgata aattatttaa aaaaccctta tttttttggc
    35461 tttttttaca ttttttcgaa aataaaccaa taaaaacaag ggttaaacta ggttaatcag
    35521 aatgcttgtg ttgcatttcg gctgctacgg atttacgaac actagcaaat agctgttgaa
    35581 tttcttccac tgtaacatct ctatttggtt taaaagtgca aaaggaagtt ttagaataat
    35641 tatctttatg cgttagttga aatccagctt gttgcattat ttgtttaacg gaggcatctt
    35701 tctcggagct tgttatgaca gttattgaat ctagctcact ttttttatgg tcaatatgga
    35761 aataacaaac gacttcctct ttatccagtc tatcaatctc ttctctaatt tcatcaaagg
    35821 atgtttctac ccgatctcct ttgggattct tatagtttat gagaactcgt ttaaaataat
    35881 ggcttttcct tccattttca tcgtattcta caccattgat aatctgtctc tcatctgata
    35941 gctctttcat cataaattcg ccacgtccac gcggattttc ccaatcaaaa gcctccgacg
    36001 taatatcgcc tttttttgcg ttctcttcta tttgtgcgag ggtataggat tttcctggct
    36061 ccatttcatg cttaaaggaa attttaaaat atgggtttga gaccagatta ttaatttcaa
    36121 gagaaacttc cgcatagggt ccatatccat gctcaattat gttttgtatt gcttctgtta
    36181 cgataatacg aatcccatct ggatctaaac ctagtattgg agcatacttt cgaatttggt
    36241 ttttgatttc aaacaaagac ccctgctttt catttatacc tgctgttaat tcttttgctt
    36301 taatttcctc tctgttgaga agaactgagt aattcaatct agcaattgtg tgttgtaagt
    36361 agagggagtt ttgaactgcg tgtaattcat cttttaggtt agtattttca aattctaatt
    36421 tactaatctt tttttttaat tcctcaacac tttcatttgc tgcttccatt caaaacctct
    36481 ttttcgatct atttttccaa tattattatt ggcaaaacac acacgatatt aacacataat
    36541 tattatgaat caagcattaa ttgtgatgaa aaaaactaaa tacgaattag acaccagtat
    36601 ttactcagac ttagattttt ataaaaaaat ttgcatgatt caaaataaca gcttcgagaa
    36661 aatatatcaa tcacacttgc ggcaactaga aagtagacga attctaatgg aagaagtatt
    36721 tccaagtggg acatttattt ttagagaccg acttgagact cttgatttac cgaaatacaa
    36781 agcaatcgta gcgcttggtg gagacaatca cttcacttat gtagctcacc attgcgatca
    36841 aaatataatc ttgggatgta attctgatac agaaacatca ttaggcgcat tactagcttt
    36901 caatcctcta tcgcttaaag ccacaattca aaaagattgg gcgaatacaa caatagaaaa
    36961 ttggtcatta ctttctgtta cgatcgaata cccagatgaa aaaaaaatca caactgtaaa
    37021 tgcggtaagc gaaatctcaa tccgaaatat gagtccagat ttaacaagca gatatattat
    37081 aaaatatgag gataaactag aagaacaaaa aagttccggt ctattattat acacaggtgc
    37141 aggctcaacc ggttggtatg cttcctgtaa aaatgatgag gatgggcaga ctagttttcc
    37201 aaaatctgca aacttctttc gagcctttgc aagggaattg agtagaaagg ctcgacagaa
    37261 ctttaaactc acagatttca cagtgacaga ttcctgccaa atcatatcag aaatgaatgg
    37321 agggatatcg attgacgcac tccccgagag aatttatcca tttccagctg gtagtgtagc
    37381 tacaatttcc ctttctaaag aaaaattaaa agttctaaca catgtaaaag aagtttaaat
    37441 aactgacatt gcacaaattt aggaaattta ggtataaatg atgtttttaa aagtattaga
    37501 aaatgctgat gaactagtag agcaataccc tactaaggct tggcttaaga aggacaaaca
    37561 aaaagaaaga aatctttctg ttcctctctt tcgccttgta aatagaggcg gcaagatgaa
    37621 acaaggagtt aaactatgac acaagaagaa ttattcgaga gagccaaaaa agtagcacca
    37681 ggaggagttc attctcctgt tagatccttt agaagtgtgg gaggaaatcc cattttcttt
    37741 aaaagagcgg aaggagcctt tttatacgat actgataata aagagtatat tgacttttgt
    37801 ttaagctttg gaccactcat tcttggacat agagatcctg aaattgagga cgttgtgcgc
    37861 gagacgattg gacttgcttg gacatttgga gcctgtgaac cttattcact agaattggct
    37921 gaattcatcg tatcagaaat tccttgggta gaaaaaattc gcttcgtttc ttctgggaca
    37981 gaagcggtta tgtcggcact tcgtgtggca agagcagcaa ctggtagaga taaaatttta
    38041 aagtttgatg gatgttatca tggacattct gacgccctac tcgtaaaatc tggctctggt
    38101 cttgctggag aagctagttc tgacagcgca ggtattggac aaagacaaat gcaggatact
    38161 ctcgttttac cgctagatga tgaatcagca gtagaaaaac tattttcgga gcatggaaaa
    38221 tctatctctt gtcttgttat tgaaccatta ccagctaact acggtttgct catacaaaga
    38281 aaagaataca tagaaaaaat ttgcagtata gcaagatctc atggtgccct aattctattt
    38341 gatgaagtaa tatcgggctt cagagttgga atgaaaggaa tggcagatta ttttgatata
    38401 aaaccagatt tagtaacata tggcaagata attggtggag gatttccagt aggagcttat
    38461 gcaggaaaga aagaattgat ggatttagtt gctccttccg gtcctgtcta tcaggctgga
    38521 acgttaagcg ggaatccaat tggaatgaga gctggactcg cagttctaaa aaaaatgaaa
    38581 cgagataatt tatatgcaga tttaacacaa agaactaaga aatttacaga cgaactaaaa
    38641 agtattttaa attcaaaagg agacaattgg gatatctctc tctttacttc cattttctgg
    38701 gctcataaga aaacggaaaa accaattaga acgatagaag aaattccttc tggtcacaaa
    38761 gaaggattcg ccaaattatt tcatggaatg cttaaagaag gagtctatct tgctccttct
    38821 ggttatgagg taggattttt ttcaaatgcg catacggatg ctattataac aaaagtccta
    38881 gaaaaagcta agaaatcgat ccaagctctt tcttaatatc atttacatag aggtgggttg
    38941 ccaaaagacc attcgaattg cgaatcaaag aaaaaacatc cgttagtgtg catacatgag
    39001 ttattatcac ttcattttca tcggctgcac cagcggatct gtttctataa gtatctatct
    39061 cggctttaga aagtgtgatg tcacaagcaa aaaagtaacc agcttcgtct aaaagaccag
    39121 cagagctata gagaggttct ggatgtaaca atcgtaaatt ttcctttgta atttccagtc
    39181 cagtttcttc ttttacttct ttgacagcca ccttccaagg atcaggttca ctatcacaca
    39241 taccagcagg atgttcataa aaaatgtcac catttgctac tcgtctttgc ttaactagta
    39301 aaaagtattt gttcccagat tctttttcaa taagacaagt tacaacgaca acaaaatgac
    39361 ctcgaatcaa aactacaggt aataacttgt gtccatcagg atcaactgca tccatatgaa
    39421 caagagtaaa aagcatttca ccattgtgct tattcactgt gtgcaattct cgaatagaat
    39481 gaattttaat attgtgcttg agaagtgtat ctcgccatga tttaaactta aaagaatctt
    39541 cgaacttttc cattctaata gagaatccta tcttgtagat attgtcaaag aaattttaaa
    39601 cattaatatt caaaaataat ttccactgga taatgatcgc tcacacgttt ttctggtaaa
    39661 tctggattag ccgaaatagg tcgaatcact ttgacaccac gtcctattgt gtttttatct
    39721 gatataatcc tatcataggc acaatctgtt ttggctacag tagtatcagt tcctgtatgc
    39781 acataccatt tagcatctct aaatacagct ttcgtaattg gtcctctact ctttggataa
    39841 tactgacagt ctgcattcat atcccctaga acaacccatt taggataggt atctctcaat
    39901 ccagtttcaa tacaggacat ctctggaatg attgccttct tctttgctgt aatatgtgtt
    39961 gtagcaaaat aaattggaac ttctttaaat ttaataatgt atggacttcg aatgatacag
    40021 cctttttcaa tatatgattc ttgttcaaac gttagttttg taggattgaa taaaaccaaa
    40081 tagcgttctg tatttcctga tgtatttcca acatccttag agactttcca tttgtaacca
    40141 gcttgcagag aattaacaaa tgcattgata tcatcttttg atacgctatt tgaagtagaa
    40201 agttcttgaa tgaaaataag atcatatcct tttagaatcc tcgctatttc cgcttgaatc
    40261 aatgtagatc tttcttcaga actagaagag gaacaattct cgcccaacat cttacaagaa
    40321 cctatatttt gaatattcca agttaagact cggatttccc ctgaccacaa agagaatgaa
    40381 attaaaaaga aaataaatag aattcgaatc atgaatcaaa gattttcaat tcctaaatga
    40441 agtcaaggtg acattccttt ttcaatagta tatttttttg ttgctattaa attcttttct
    40501 ttaaaatctc aaaatactta ccaaaataag cataaccaaa taaacaaacc tcacaatttt
    40561 tttataggat gtatttaggt aagagtctac ttgtaaactg aattatttat tttaaaaata
    40621 tatttcagtc tttgtcaaaa tcgaggagtt ctttatgaaa aaatttatat ttttatttat
    40681 ttatttctat tattcgatta ttctttttgc caatggcaaa cttacaatag cagtgattgg
    40741 acaagaacca aatcttcgcg caaacattga atcaacaatt accaattcag attttttaca
    40801 ggcagtagaa agaaatcgaa tcgatacaat gctcaccgaa atgaagttgg gacagcaagg
    40861 agttttaaaa gaaggtagct atgccaaagc tggagaaacg ttaggtgcat cctatatagt
    40921 cttagttgaa aaaaactcca acgcttttag attagttcac acccaatctt ctagaatttt
    40981 aggtagctgg actggatgga atgacaaatc ttcacaagag tttttaacaa ttctagaaag
    41041 agagaaatca ttacaagaac tagcaaattt aaaatcagca ggtaagaaag attataaaat
    41101 tgaagtcaca aatgcgatag gatctaaaga aaatggagga gtaaaaattg gagatgagat
    41161 taaaatagaa tttatagtaa aatcaaaaaa agaaaaatat gcttatgtga ctattttagt
    41221 ttatggacaa gacggaagca ttactcaact atttccaaat aaataccaat ctgataataa
    41281 aattgaaaca aatatcgatt tctcttttcc tccacctgac gctccaaaga aatataaact
    41341 aatcgcatct caaccaatag gagaagatac aatggtaatt atagcttctg attcatccgt
    41401 agcgcttgag gatggttctc ctctcggaat ttactttgga aatacaaaaa gcttaaaagg
    41461 aactaaaggg ataacacttc aattagataa aactggaaca gtaagatatg acgtaaatcg
    41521 aatcgttttt tcaattaagg aaaagtaata taatgataga gtgttcaaaa ccaatacaga
    41581 tcaaaaaggt ttctaagaaa ggaaggagaa aatttgacct ttccaaaagc caaaggaatt
    41641 tcaacgtagg aatgattcat tcttttggtt tttggcgaaa aagctgattt gtctgaaaga
    41701 gattttagat tccttctctt tttatcacat caaattaaaa agttcttaag cttaagttat
    41761 ttgaaccgat aagtcatact gtgagattag ataatattga ttccctacaa tccgttctgt
    41821 ttagaattaa ggaaatttct caattagcca atcaaataga aacgaatcct agtaagcagt
    41881 tctctgaaaa accggaattc gcaaaggaat tagacaaggc attatctgat ttacaatctg
    41941 atattcaaaa accaaaccaa atcgctccga aagaaagagt caacctcgaa cccaaacgag
    42001 atgaagattt ggcagaatta aaaaactcca aaaagacaaa gtcaattgat caaatcatcg
    42061 aaacagaagc aaaattaaaa ggtttagatc ctgatttggt cagagcggtt atcaaagcag
    42121 aatccaattt taatcctaaa gcagaatctc caaaaggagc tatgggctta atgcaactta
    42181 tgccaggaac cgcagatgaa ttgggagtag aaaatccttt tgacccaaca caaaatatta
    42241 agggtggaac caagtatcta aaagagttgc tagatacttt caaaaataag gatttagcag
    42301 ttgccgctta taatgctggt ccgggagccg ttagaaagta taaaggaatt cctccctact
    42361 ctgaaactaa aaactacgtc aaaaaagtaa acagctacct aaacgaatac agatagtatc
    42421 ctttattctt ttatggaaaa tttgcatttc gattttgaag ctctccgtga caaattagag
    42481 tatgatgacc cttttataaa gcaattctta gaaatgctaa aagaagagct cgaaatctca
    42541 tttacgaaac tcatagaaca aagcaaaaca aataattggg aaggcataaa aaacgcagca
    42601 catcgaataa aaggttctgc tctaaatgca tgtttcaata agctaggaaa aatatctgcc
    42661 tctcttgaaa aaatggaaga ccctacccct gaaaaaatca tgagtcttat aaagcaaatt
    42721 gaaatagaaa ttgctttagc aaaagaaacg attgaagaaa aaataaataa ttagaaatcg
    42781 aaagtattag gattaaaagt ttttagagag gaaggaaaac tcgatgcagt ttcctacatc
    42841 gagttatttt catttttact tcagctgttt agcgtcgtga gaaatcactt tcttacgatc
    42901 tactttcaca aagcctttta ctgtatgaac ggtaaaagta ttttcatcca ttgcagtgat
    42961 aacacctaca actttacttc ctccgtcgag tgataggact tccaagcgac cgtatttatc
    43021 aaataaactt tgctctgttt ctgctgttac agttgtcgga atagttgaag tttggtcttc
    43081 ctgttcgtct ctgccttcaa tgattttaat atctgcatca ctgattccag aacgtttaaa
    43141 gtttccacct ttggttaact cctgaccaga ctcttttcct tcgatgatct caaagggctc
    43201 agctgttaga gggacttctt ctttgtgttt tgtaatacct accgaaccat ctagaacaga
    43261 tactcttgtc acatcatctt ttgcagcatt tggatccaca accactaaga atcttgttcc
    43321 acgaacaccg gcaataacgg ttggagtaac aatattaaaa ttgtctgttt gcttttgctt
    43381 agtaacgtta gctacaagca tacctttctt caaggaaact tcttctgtaa tgtttccatt
    43441 ggtttcaatc agtcttttta cagcaacttc cgtatttgct ttgattcgaa tggaagatcc
    43501 atgagagaat tccaagtcaa tggcagcttt ctcccctgta aaaagcaatt gtcctgtagt
    43561 tagataattt cctacttgcg gttttacacc ttcttcagaa atactatttt tcactttaac
    43621 atcacccaac acaaaagtta ccttagcttt gagttcacct gctgcaactt gaggatcttt
    43681 cggtttatca ccattcccta cagtgataaa aattcctaac agaaccactg ctgcagctcc
    43741 taaaatataa attatctttt tattcataaa actaccccga tttaatttta ctatattttg
    43801 ttcgtttgat ttgtaatacc tatcaaggat ttcctttgaa tccggaagag atactgaata
    43861 ctctcctggt tttgaaagaa gatcgttaag tcgctgaacc tttgtagaca cttcattgtt
    43921 ttttctatca atcaaagcat cataaacttt tttttcaaat tctgaatatt cttccatatt
    43981 aatacactct ctcttaattt atggtgcaat tccattttcc tcggcaagtt gaagcaaacg
    44041 agctgttgcc tttacaacta acctagaaac agttgaaatt gataattcca tcacttctgc
    44101 aatatcagct aacgtcatat cattgatatt tttcatgata agtgctgctc tctgatcctc
    44161 aggcagtaat gctaaacaat gccgtaatct aatttccata tctttcaatt cttctctctt
    44221 ttcaaatgaa gtcttttgac tttgatatat atccacatta ttggatgcgt tctcttttac
    44281 tgtagaaaac ttcttactat aattgattga gttgtttctg gcaattgtgt agagaaccat
    44341 tatggctttt tcaggtggga ggttactttc tccataactt cgaaaatagc ttaaaaaggt
    44401 ctcttgcatt aagtcagccg caacctcctg attattggaa tatttataca agaagtcaaa
    44461 aattctttta ttaaacctct catatatatc tcccatgtaa tttctattat cttgcatccc
    44521 ttcatcatct ggaatgactt ctagaatttc aagataaaaa agttcacatc cttctgtcta
    44581 aactaacaag ttagaactag aattttttca aaatgaatca tttattttta aatagtgctt
    44641 tagataaatt ttttatctga tttatttcat caggaagaaa aggaacaaat tcccgatgat
    44701 atccatgaca gacagtttta acggaacaat atttacaatg cccatttttt gttaagttat
    44761 agggagctgg cggatattta tcatttacaa gtcctgccca aaatggatca aaaattttat
    44821 cagaaaacat ttcaattgaa tcaatcctta gtttaggttc aatatttcga tttaaatgta
    44881 aataggctgg aaatatattt agtttcatct tgaaaaactt ctccaactct tcttttccat
    44941 ttgaattttg aacaagatat gcagtataac ccacatttaa tttttttata tcatctatgt
    45001 cttttgctgt aattatttga gttaaataga gatttgaccc atctagcagt aataaatctg
    45061 ccgatgcact gatttgcttg tttggtaaat ctaatgttga aaattttaat gaattatttg
    45121 ctttaattgc tttagaatta aaaactgttt tcccaaatat cttatatttt aacattattt
    45181 cgcggtaaat cgtcgttacc ctctcaagat attctaaaag atcaaactct gtaatttttc
    45241 caagaacacc aaatggaact tctccaaatt cgactctctt gggacttaaa atttttttag
    45301 ttaactgttc agcagaaaga cttggatctt ccaataaatt atacagggct tcttttaaaa
    45361 acaaatgcca acgaaaagaa tctatactat caatatcccc ttctgtctct tcctcttctt
    45421 ctgtttgaat cgaataatca aagaaaaact ttttaggaca atgaatgtat ttcataaatc
    45481 cagctacaga catattttga ctttgcgtca tttctttata aacgcccgta gattcatagg
    45541 atttctgaaa gtaggatttg attttttgct ttaagaattc atctcgagta attaaatcga
    45601 tttcttgatt taatacttca tttccatccc agttcggata ggcagctttt aaaaaattta
    45661 actctgcttc ttttttctta agatcaatta ggctaaaaga gtccctatca aacgcattta
    45721 gatcagattg tcgatattct aaataggaaa acaatggaat tttattatat ttcttttttc
    45781 catactcagt tcgaatccaa tccaattcga cagccggata aaactctcta tctttaattg
    45841 tgtcaaggct gacataacta aaaaaatact cctgagcagt atgatgaaat atatgagcaa
    45901 aataattttt gactttcaaa gaagaatttt ttctagataa ttgaatattc tcctcttgca
    45961 taagggattc aagacgagtc gatgatctac ccaacctgcg atcatctaag ccaaccaagt
    46021 aaacaaagtc aaatatagga tcgactgcat ctaaaagttc tcctacaacg actccattct
    46081 ttaaaactct agaatcccct tctgtccatg aatctatttg ctctaataaa gtttcaacaa
    46141 agtcttcgag cgtaaaatga atattatccg ctttaattga ctccaactct tcctcaattg
    46201 cttccagtgc ggataataag ctgtaaacct tactctgacc tctttggttt aactgaatca
    46261 gtctttctgg atcagtagaa ggtttatttc tcattgaagg atttaaatac gtgtcgagta
    46321 gaatccgaaa gaatcgactg aaatctttta aactagaaaa gtcctctttg aatccaatta
    46381 agtcctgtaa gagagaagaa ctaatttcta ataattgata agcctcttct actagctctg
    46441 gctccagact tgactcttca tcttgatgca ctagaataga atccaatcgt ttccagaggc
    46501 tttcccaagt cataagatta gtctctctaa gaccttctct tcttttatga tatttatcca
    46561 aaaatccaga aagattcatt ctagcgataa tttgattcca aacatctgga ttgatatctt
    46621 ctctactctc ttgtatttct ggatgaaaac atggattata aaacaatgaa aagattgtat
    46681 ctttatcaaa gtctgaaacg gaaattttta gtagggatac aacggctgca atataaggag
    46741 tctttttttt tcgaatatct tttgtaaatg catacggaat tttcattcga tcaaaaacat
    46801 tgtttactat tagtgcataa tttgtatctt ctgttggtaa aacgagttta attgatgtta
    46861 aacgaaaatc tggattcgat ttatttttct ccatcaaacg taaaatttca ccgccaataa
    46921 actcaatctc cctataagct tcttgagact catagaatcg gagagagtcg ggattgatat
    46981 tgattcgatt agaagcttga atcaattcat ctaatttcaa agacttatca ttttctgcat
    47041 aagtaaggac atgccattca ggtaaaagtt ctctgagctt aacgtccttt ttagttaata
    47101 aatctttttc tttatgtatg actgaaagaa ttggaacagg aaggaaaatg gatattttaa
    47161 tatactgaga taaaattttt agaatattga agtgtaatgg atcgatttct gaaacaccat
    47221 ataaaataat ttcctcacca aatttttgct ttgcatcttt ttcggataaa aaattctttt
    47281 ggtctctaca taaatcatat agatgttcta actcaatttt cttaggtctc tttttcttga
    47341 taaattcacg aatgacttca tagtattctt cttttatatt agaaggaatt ttttccttac
    47401 tgattgaatc agataaaagt gaatccttcc aaaatttaaa tatatcagtg gctaattgtc
    47461 gtaaaactct tttatttgtt ttggaatgag tagattcttt tttagtttta aatatatttt
    47521 caatgagaag tatacatgac aaatctgaaa tcaaatcatc ggctgatatt ggctttttta
    47581 aaaattctct agcaatggtt ctatatggag tcacctcaaa caatagagaa ggaatattat
    47641 cttccactag tttgaatgca atataatctg ttgtaagatt ttctcgagtg aagatgcgaa
    47701 agttgttttt taatatttga acattgtccc tatttttaaa ctcctctagg cgatgcaata
    47761 atgcctgata tagttcagaa ttattcggac aaattatata ttcaatgtta ttcgagcgag
    47821 attcgttcat acttgagcgg ctcttgtatt ggattaaaga aaaacttagt tttacaagct
    47881 gggcaggcat aattgccttt agctttgagt cttagtttag cgttgcaaga gggacagtat
    47941 gtataaaata caccttcttt tactagtgca gaacgaactt caggtagagc agagacagtg
    48001 ctcacatttt cttgtgtaat atccgatctt gattctaaaa agttttttgc ctcttcccgc
    48061 aaggcaaata tatgcattaa tgatttatca atattttctt ttaactgatc aacaaaattt
    48121 ccatttagac cagaaaatgc aatttcagct ccaaattctc gtaggtgata aatgattttc
    48181 ttaagaaaaa ataacccttc tggagaaatg gaatcaatat ttgtaaaatc aaacaggaca
    48241 gcttttgttg tgtgggcaat cgattcaatt ttcttccata catcctcgcc tagcttttgc
    48301 ccaaagtttc ctgataaatt aataactata tgtttggctc tgctcaatat ttcctttccg
    48361 acaaaattag attctgaaat tctggatatt agtaaataaa attaatagta taaaaaatat
    48421 atacaataaa aaatctattt taatctcttt tttttcgtgg gtatttgtaa atgtttagca
    48481 gaagatattt aatttttccg attgatgttg tttttatgac aatatcctat attacagccc
    48541 atttagttcg ctacgaaagt ttggagtttt tagaagacca agaacgcttt tttatctcac
    48601 taggcattgt aattgtttct agatctataa ttttcctata ttctgatatt tatcgatcag
    48661 tctgggcata tgcatccatt catgacttaa tggaaattat aaaagttact ttaatttctt
    48721 cttttgtctc aactactgct cttgttttat acaatcgttt ttcacagcat tctagaatgg
    48781 ttccaatctt agatactcta ttacttttaa gcctactctg ccttaggagt ttttcatgga
    48841 gaattatcag agaccaatac attactaaaa atataacttc agggaagcca acacttatca
    48901 ttggggctgg gaaatctggg ctgatgcttg tgaccgaaat ccgaagacaa ccagaactaa
    48961 atctaaaacc aattggtttc ttagatgatg atccagccaa attaggagct catattcaag
    49021 gtgtgccagt gctaggacca atagaccaaa tcgaaagatt tatattgagt tatagagtgg
    49081 aagagttgat cataacgctt ggcgcccttc caggaaaagt tattagcaat atcatacaaa
    49141 tttgtgaagc gtataaaatt catgcaaaga ttcttccttc tatcggtgaa atatttgcag
    49201 gtaaaaaatc ttccttcaac cagttaagag aaatcagagt agaagacctg cttggaagag
    49261 atccagtgaa cctagaaata gaatcgatta aatcctactt aaagggacag gttatcttga
    49321 tctctggagc tggtggttcc attggaagtg agatatgtag acaggtttct ttttttgaac
    49381 cagaaggact aatactttta gattcagcgg aaaccgctct ctatcatatt gattacgaat
    49441 taagaaattc ttttcccaat atccgatatg aatcgattgt tggcgatgta aaaaatctat
    49501 ctagattagg ctatatattc gaacaatacg cacctactgt tgtctttcat tgtgctgctt
    49561 acaaacatgt cccgttaatg gaaattaatc cctctgaagc agttttaaat aatgtaatgg
    49621 gaactaaaaa tatagcggat atttctagat tgtcgggtgt tgaccggttt gttttgattt
    49681 ctactgataa agcagtcaat ccagtgaata taatgggagc atctaagcgg gcatccgaat
    49741 tgtatctaca acatataact cctaactcaa aaacaaaatt cattaccgtt cgttttggaa
    49801 acgtgcttgg ttctaatgga agcgttatcc cccgtttcaa agaacaaata gagaaaggtg
    49861 gaccggtgac agttacccat ccagacgtca tacgatattt tatgacaata ccagaagcat
    49921 cgcaattggt tttacaagcg ggtagtatgg gagaaaaggg cgagattttt atactagaaa
    49981 tgggagaacc tgtaaagatt ctagaactag cagaagaaat gattcgacta tccggttttc
    50041 agccatacaa agatattaat attgagttta ctggtttgag accgggagaa aagttatacg
    50101 aagagttact actcaatcta gaagggatca aaaagactca ccatcccaaa atcaaaattg
    50161 cggcctccaa tacaaatcta aaccaaatgg ttttcctaag taagttaaat caactcttca
    50221 gcttagccaa agcaaataaa aatgcagaaa tctacgattt atttaaagaa attattccag
    50281 aataccaaaa acacattgat tatatttcga ttaccaaaaa gaattaaaaa gaattatgga
    50341 acaagattta actgaaacag aaattagaac attaagagat tttaagatac aattatttga
    50401 aacctactgg gagaaatttc caacctttta tcttcatgtc ctaccacatc ccaatttaga
    50461 aatgggaaaa aggggtttag ttggagcaga aaaagaatct ggtgttgttc tggcatttgg
    50521 atctacagct gttcgagata tatcctcaca ggcagactat ttgtatgcag aactccaatt
    50581 tggattcaat tgggaaaaat tgatcatccc gtgggatgca gtttttagaa tctacgataa
    50641 aggacaaaac tctatcaccc aacttcgtat actcacagac gaaattgatt ttagcaattc
    50701 cgaatcaaaa acaaaaaaag tagaaaagaa aaaagagaca ccaagtgaat cgaaagttat
    50761 agaagttgat ttcacagcaa ggaaaaaaga atagaatgaa tcaatttaaa tggatagtcg
    50821 ccggagacat tgatgggttc ttcggattga tgcttgataa tttactacaa ctactagtat
    50881 taactgcgtt atgctcattt gtttgtggga tgcctcccga gtttgtattt tcagttgttt
    50941 tgccaggtgt aggtatatca ctgttattcg gtaatatttt ttatgctttg caagctcgaa
    51001 agctagcgat tcaggaaaat cgaaatgatg taaccgcctt gccttacggc atcaatacca
    51061 tttctctttt tgcttttatt ttttttgtca tatttcctgt ttttaaagca acaggtgatt
    51121 ataaaacagc ttggaaaatt ggtttgcttg catgttttat cagtggggtt attgaatttg
    51181 ctggttcttt tatagcacat tatattcgaa gtattacccc tagagccgct cttttatcag
    51241 cgttaagcgg tatcgcaatc acttttatct cgatggagtt tatgattaaa acttataagc
    51301 atccattagt tgcctttcta ccttttggaa ttattctttt acaatatttt ggaaagttta
    51361 aatatccatt tcatatccct ggtggttttt tatccatcgc cgcgggaacc agtatagcat
    51421 ggctagctgg ttattgggga gaaccaatga tgaaattaga tgcacttaaa aattccttta
    51481 gtcaggctgg aatctatttt ccaaatctat cgatgacgga tttatttgat gtgtttactc
    51541 tccaaaatat aaaagaatat tcttctgtaa ttctgccaat gggattgttt aatgtgatcg
    51601 gttctcttca aaatattgaa tcagcggaag caagcggaga taaatttgaa actagaagtt
    51661 ctctagcaat gaatggagta ggaaccatca tcggttcttt ttttggttct cccttcccta
    51721 ctacaattta tatcggacat cctggatgga aggctcttgg ggcaagagct ggctactccg
    51781 tgttaaatgg gatttttatg acgatccttt gtttgttcgg attaatgagt gtagtgcaag
    51841 cgttagttcc aattgaagct ggtatggcaa ttatactttg gattggaatc atcatagggg
    51901 ctcaatgttt tgaaacttct cctactcgac atgcacctgc aattattctt ggactatttc
    51961 ctgcactcgc tggttgggga gtattgatga tccagagtgt tttcaatttt gcaaatggag
    52021 aactagctaa aatcttggcg aaagaaaaca taactacaaa gtattctatc acaatgcaat
    52081 ctgtttcccc aggattggaa tttttgccct actcacttgc tgggattctt tctctttcac
    52141 aaggattttt aatcatatct atgatttggg catctatttg tgcctttatt atcgatcaaa
    52201 aatttagaat tgcaagctat tggtgcttag caggtagcgt tctttcgtct actggaatca
    52261 tacattctta taagctagcg gataacgcaa tattaaatga atatagtttt ccatcgaata
    52321 tggaatttat tgtagcctat tttctattag cagttcttct gtttagtgcg tctttggttt
    52381 ctggtaatac aaatgaaaaa tgatttaatt aaagataatc atcaatataa aattctagat
    52441 gaagaattaa caaaaccaat tccaaatttc cgaaaaattg aagaagcaat cagtaatatt
    52501 tctcctgcta ttttatcaat ggcaatttta gaagatgttg gatttacata tctaatcgat
    52561 tactatctac gacatataaa tattcaagtc gttaagaaaa tattacaaaa tccaatgttt
    52621 agccatgagg ctcttatcga attattctat tgccaagtaa ctgcttatca taaggcaatt
    52681 cttcaaaaaa agcctcttcc tgaaatgttg acctcttact ggacaacatt aagcaaagct
    52741 gaatatgcaa ttctttttaa gaatttagtt tctagaacga gcaatatcca tattgcgaaa
    52801 aatttatttt caaaagttga tctggtctat ttaagaatga tgacatcatc aggaacactg
    52861 aaatcagata gagttctcaa ctttttcaga aaacttggtc ccgacataca gaaattagcc
    52921 gctcaagaca tgcatattta tgattatgcc tttgaattgg cagttgcaaa ctcggatgat
    52981 gattttctag cctttctaga tgaatataca gtagtatttg ttcaattgcg acttgtttct
    53041 tcttttgtgg atgaaataga gtcagagatg aaaaaaaatg atgggcataa actaccttat
    53101 tttcaaattt tacagatagc atcgcatatt ccgcttgatt gtttaaaaat atcgttggaa
    53161 atttttcaag agaaaggttg gattagcgca aatgaaaata aagcgattca agaatattgc
    53221 actaatgcga aataagtaag tctaattact aggagtttat taaaaatatg tggttcaaac
    53281 gaatcggatt attttttctg attaatattt tgataatggt gactatctcc attatcacta
    53341 gcgttctcgg cgtgagaggt tatatttcaa atagcggtct aaatatgact gctctgctta
    53401 ctttgtgctt gatctggggc atgaccggat ccttaatttc actcgcactc tcaaaagtca
    53461 tggcaaaatg gatgatggga gtagaaacca ttgatccacg cggtccctat ggacatcttt
    53521 atgcttctgt tcagagactc agtcaagcag ctcgtatccc tatgccagaa gtaggtattt
    53581 acaattcacc agaagtaaat gctttcgcaa caggcccaag tcaatctagt gcgttagttg
    53641 cagtatcaac aggacttcta gaaagaatga atacaacgga agtggatgga gttcttgctc
    53701 acgaagtatc acacattgca aacggggata tggtaactat gactttgatt cagggtgttg
    53761 taaatgcgtt tacaatgttt ttagcaagaa tcataagcta tggtgtaact atggcaatca
    53821 gcagaaatga tgatgatgga ccatctgtat ttagttctgg gatttatttc atcatcactt
    53881 tggtattgga tattcttttc agcatattgg gatcattagt tgttgcttac ttctctagac
    53941 agagagaatt ccgagcagat gctggaggtg ctaaattagc gggaagagca agtatgattg
    54001 cagcacttga aaatttacag agaacatacg aagttgtcga tgacagccgt gctccaagta
    54061 tggcatcatt aaagatatct tctcataaag gcggatggtt cgccttattc tctacacacc
    54121 caccactcga agacagaatc gcagcactaa aaaacgctcg ataatcctat ctaattgtcg
    54181 tgtgctaaaa ccacacgaca aaaaaatcct ttcaagccct aaacaaaaaa acatcttggt
    54241 tttagagggg taaaaacttt tggcatttca tttagaaaga agagcaagag ctagaaagcc
    54301 tagattatta atttcattct ctttgttaat attattgcta cccgtttcaa attatatcgg
    54361 gatgagtatg aaatataact tcccttggta tgcccctttg cacttattca gcaatgcaaa
    54421 cctacctgag tggatattac taatcagctc cctactctcc gggataggac ttttctatat
    54481 taaacgttgg ggttggtggt tatttattct atcttccttt acttttattg tgtataactc
    54541 tttcggtttt attacaattc cagaatcagc aacaaaagga ccattgattc aaacttttat
    54601 tgttactgct gcactcgctt attttctaca aagagatatt tttataccat acatgaagtt
    54661 gtataaacga ggatggcgct tacaaaaacg ttatccgatt tgcattaatg taaatataaa
    54721 cgacacaatt tataaaacag aaaatattag tcttggtggt attttaatta aaagcaatga
    54781 gtgtaacttt ggattaaatt ccgaggtaac ggtttatttt gaattaagta aagaaggatt
    54841 tctgacaaaa tcaggtatcg ctagtattac tcctgggtat ggcttcgcaa ttgcttttcg
    54901 aaacacagat agatcattta aagaaagatt aaaaaaagcg ttattgcttt ttgaaaaaaa
    54961 attagatccc tgatactaaa aagagacatc tttaaaactt gtgacttata tagactacaa
    55021 ggaatagaac ttgtcaatcc cttgtagtct aaaggaacta ctcggttctt aaatcttcta
    55081 ctttaatgat aaaaacctta agcagttaaa tcctccatga tttgactaga tctctttaag
    55141 aaatttccca tatcagcctc gctcatcacc ttagaggaaa ttaaagcaag gtcataaata
    55201 tgttgagcaa tcaattttgc tttttcagga tttacaccag cactcattct aagagcattt
    55261 tttactaact tagaacttga gttgatcaac aatgtatgca tctttaacaa tgcattctta
    55321 tcatggttca ttcctaggtt catttcagag atacgacgca tatgctctgg taaaatcaca
    55381 actgcaggaa catctcctgt ctttagagat tcaactttaa tctcaagccc ttcccttcca
    55441 attgcttctt tgaagaaatc ttggatacga gttgattctg tcttattgtt ttcgtcgacg
    55501 atctcagctt ttgttgattt atcaatcaat tggtctgtga tctctgcatc gactctttgg
    55561 aatctaacgt ctggattttt accctctaag tattggatga aatgagaatc gattctagta
    55621 tctactagga ctgcttccaa gccacttgac ttgaacaatt ccataataga agatgatgct
    55681 tctacctcag gagcgtaata aactttcttt tcatttttat ctttatttcg ctctaagtat
    55741 tcatccaacg ttgtgtaatt accattagaa gatttaaata atacacagga ttttgctgag
    55801 tcgtaaaact tttcatcact gataattcca tactttacaa aaagagcaat ttcatcccag
    55861 tttgcttcaa agtttgttct atcttttttg aaatcttctg ttaaacgatc tgctatcttt
    55921 ttaatgatgt gagcggatat tttcttaacg agtggatctg tttgtaggta agatcgagat
    55981 acgttaagtg gtaaatctgg tatatcaatt gttcctttta gaactgttaa gaattgaggg
    56041 attaattcat tcgcttcatc actcacaaaa acgtgattac aatataattt tattccattt
    56101 ttagaaacat ctagttcatg ttttaatttt gggaaatata aaatcccctg caatctaaat
    56161 ggaaaatcta catttaaatg aacccagaaa tgtggctcac cagaaaatgg aaatagataa
    56221 ttgtaaaact caatataatc ttctttttta agggaagatg gagactctga ccaaagtggt
    56281 tttctcttgt tagcctcttc tgcttcaact gtgatcggaa cagataagaa gtcacaatat
    56341 tttttaatta attcttttaa tttccattta tctagatatt ctccagaatc tgaatctaga
    56401 tggagagtaa tctttgttcc acgctcttgc ctgtctattg tagaaagttt aaaatcggtt
    56461 cctgattcac tttcccattt aacagcggtt tctccttttt tataggattt ggattcaatc
    56521 actactttac tagataccat gaaggaggaa taaaacccta aaccaaaatg tccaatgata
    56581 cctgctttgt tatcagctga atcgtatttt tttgcaaatt cctcagcacc actaaatgca
    56641 atttgattta tatatttctt gatttcttcc tcgctcatac caattccatt gtcctctatg
    56701 gaaagtgtgc gagcttcttt atcaaaagta acggaaatct tgtaatccgt tccgccttca
    56761 aactcttccg taagcgctat cttttttaat ttcgtgatag cgtctgtcgc atttgaaacc
    56821 aactctctta ggaaaatatc tttctccgaa taaagccatt tttttatgat gggaaaaata
    56881 ttctccgttt gaactgatat tttacctgac tcactcatat taatttattc tcctttagta
    56941 tatccttaat taaatttaaa atgatttctt tttggcgggc atcgctttga gagtaaattt
    57001 ctgaaaactt tgcattaccc gcttttgatt tgattcgaac taactcttct actttcgctt
    57061 tgtttaaacc tttttcttgt aagaagactt ttagtaaagc acctttttta ttaccgattg
    57121 ctttatccat tagatgtatc tcaggacttg ttctaaactt gtccaagggg attctatgaa
    57181 atattttttt tgcagaagct aaaatttctg ttcttaatag gtaaatggca aataacccga
    57241 gcccagtaac cacaaaataa aatccccaga gttgaaaatt tacactacta acagttggaa
    57301 tttcctttgg tgtaaaaata ggagcaggct ttacccggat ctcaggaaat gaaaaagaaa
    57361 tagtttcata agattctgta tttggattaa agaaaacaac ttccacagaa cctagattta
    57421 agattccctc ttctttcggg aaaagactat atcgaaaggt ggagtctgat tcaaatccat
    57481 attcacctgt ttttaattta gtaaattttt taactttatt ctcatctaag aatgttatat
    57541 ccgctttgca ttttttttga gggcaaaagt tttgcaaagg gtctttaaac attgccccag
    57601 tgccttgacc atttatctgt attgaaaatg gaatgggaac tccgacggct gcctctttag
    57661 ctgtaaatgc cagtttgatt tgataatctc ctacttctcc tgaaaaattc gtaggtctag
    57721 gatttggaat atctaaaacc tcaaccgatt ttttgaaaga atctttaacc ataggagaaa
    57781 agtaagaaag aggatttcct tctatactaa actttgcacc gcctaattgg tatttgcctt
    57841 tcctaagtgg agtcaatgca tagatttcgc gattataagg aacaatttta tactgaactc
    57901 catccaatga agcgttagcc ggtagattta tggaaacatt tggcaataat tctgttgtaa
    57961 aaaagggaaa ttcgagtgga tcttgctcat ttctctcaaa gttgggtctt tctaaatttt
    58021 tataatataa agtaaaactt ccaataacag tttcaccaat ataaaccctt gatctacttg
    58081 tataaaagcc tattttgata tcatcctcgt ttggatttgc ataggctctt tcctgaaacc
    58141 cgggaaagtt ccctgaaaaa aattgatcaa aaattgaatc cattggtttg ggtctcggag
    58201 tatatttttc cggttttaca gtaaaagcga tagactctgc agtaaatggc tgttgattga
    58261 tagaaagaat aatttttgga gtcgtcagat ttccagtctt tgcagttaga atccgaaatt
    58321 taactatttt tttattggtt atttttgaat tgataatcgt tgtgttgtct tccagaccaa
    58381 tgtattcagc agttactcca ttctcagaaa aaacattttt agatagtgaa acctcggggt
    58441 cagatccacc tatctcaatg cttaattcga gtggctctcc ttttaatata gtccctttat
    58501 taagagctat tttgggattt gcaaaaagac ttccagtaaa taaaagaaat aaaacgattc
    58561 taccaaaaaa tatcatttct atcctgtggt ctctcattca ttttttttct gttaatttta
    58621 ttttgtttag cagattctag tattctctct gcctcttcct tgctcatctt ctgattggac
    58681 tctttgttct tttcgtcctt ttgattattt tcattttgct cagaagtttg gttctgttga
    58741 ttctcttttt tatttttaga ctgttcttgt tttttatttt gttcagattt atttttatca
    58801 tctgaactct ctttgttttc ctgttcttgt ttttgatctt cttgattctg ttttcttgac
    58861 agcatttcta aatttttgcg cgcaggcaaa aaatctggat tggatgaaag tgcatctaga
    58921 taggaccgaa ttgcattctt cttatcgcct aacttagcat atgtattacc tttgttgtat
    58981 aaagacttaa ccctcaaatc cttatcattt gaaagagcgg atttttcaaa atacttgagt
    59041 gccaacttat agtcgtttaa cttgtagtat cctgtccctt tattaaaaaa aagtcgatca
    59101 tcctcttttg cttgcgactc tgcttcgttg aaactctcca atgctccctg ataattttga
    59161 gacttgtatc gttccactcc ttcttttaca tttttcccta ctggatctac atcaaacgca
    59221 aaaatagaat gataggtaat taaaatcagt attaataaaa caaaggctct catataaata
    59281 tagacttttt taattcttag gggaatcaat cttatttttc agatttattc tcccgcataa
    59341 aaagcttcct cagctgctaa tataaaacct gttttgggat caaccactct acaattcaat
    59401 ccgtatccat tattaaaaga tgtaaccata cccgtcacta ttgcattcaa acctagtagt
    59461 tcaccgattc ttctaatttc agccagatca ttggcggaac tcaaactcaa cttattatcg
    59521 agtaactgtt tggaaaataa aatacgatct aaaactacca tatttcctct tctcgctaaa
    59581 tctgttgtca gtttttcggc aaataaattt cccagtttat gttctttgcc ctcggtagtg
    59641 gtaaaggtaa gaacaatgag cctagaattt ggttgaggca ttttctttga aaatgtctca
    59701 gctatttttt ccattggagt ctgtgaattt gtttccgttt ggtttggttg cggattctta
    59761 gtgttattcg aaaaataaga acaagaactt tgcagagaaa aaaataaaag aggaattata
    59821 tacaaactta gattttttcg attcattggt ttatgaccta tatcattcag atttattatc
    59881 aataaaataa gggctaatga atttcaaatt ctcttgcttg aggataatca tgagtaaccg
    59941 atccaagccc attgcaatac cagagcattc aggtagacca cttgtcaatg ctgctaaaaa
    60001 attagtatcc atttcgaaag cttcttttcc tagctccaat cttttaactt gttcctcttt
    60061 aaatctatga atttgtgttt ctggagatgt taactcataa aaagcatttc caagttctac
    60121 tccattccaa tagatttcaa agcgtttcgc aaatccattc tctactcgag caagggcggc
    60181 aagttcggca ggataatcat agacaaaaac gggtccacct ttcggtaaat ctttttcgat
    60241 acagttcaga aaaactaaaa aaaacaaatc atcataatac atggactcat aattattaag
    60301 tgctttagga aatttttctc gaagagttat gattaaatct tccgtcgaaa aagaacgccc
    60361 tgtcttctcc ataaaaagtt gattcataga aactcgaata caagattctt ttttaaaacc
    60421 aaagtctaca aaatgatttt gtaaatattc aaatagagag atacaaacat cgattagccc
    60481 ctgctcatca atacctactt gatagaactc taacattaaa aattcttttg tatgaagtaa
    60541 acccttctct ccagaacgaa aacaatgcgc aatctcatag attttatcca agccaattgc
    60601 taaggcttgt tttagagagt attctggtga tgtaatgaga tagccttctc tatcagtatg
    60661 tggggatttt acgagcatgg gatctaaata aggctccata cccggaactt gttttaaaat
    60721 tggtgtatca atttctaaaa aaccattctc ctgaaagaaa gaacggattg caaataagta
    60781 gcgggctcta tactttagta attgattcga taggagttga ttcattgaac aatcgcttca
    60841 aacattttta tataaaacaa aaatctgata cggctgtgat tatacaatgc ctttttgaaa
    60901 ctattgatga gtctgtggaa gaagaagcag ggtcaatatt caatgtttta tttttgagca
    60961 caaattttga tgtagccata tctttagaaa atcccgttta tattacacca aattttatca
    61021 aattactgct aacactcggt caagaactgc atataaatgg aagacaatta acagttttaa
    61081 atgctccgga aagtatggtt cgatatatca accgatttca tctagataca tttatcaaaa
    61141 tactaaatga tagagattcc agccccgctt aacttgtgat tatcccattg cgttacttca
    61201 atattcacac ctaggttgcc gattaacgtt agagatctag aactcaattg gagggctttt
    61261 ttagattcat ccgaatactc aatagaacaa tagagagtcg caatatgttt ttcattcaat
    61321 tccttgaatt catttcgaac aggagcaatt ttctttaaga gagcgactac atgggattcc
    61381 aattcctcct ctggagaaag attggaattt aactgccaaa ttgttttagt agttccattt
    61441 tcatccacag ttccaaaaaa gtctggatgt attttcaagg tatcattgat ttcacttggt
    61501 tctaaattcg aagtagataa gactaaataa gcacaacttt ttggattctt taacataagt
    61561 cgaatagtta tatagaatta taaaaattca gcaagcaatt ttttgactag ataaaaagat
    61621 tttaaaactg ctatcctatc tctttatata atttataaaa ctgatcaatc tttgcagcca
    61681 aggaaaaatc aagaggtccc actcccccaa tcgaatgggt atagacttgc actcttactt
    61741 tatcccattt aatactaatc tcaggatgat gatcgagtcc ctctgcaaca aatccaacag
    61801 aattaacaaa ctgtaaggag gaaataaaat tctttaataa aaactccctt acaatataca
    61861 gcccttcgta actccaatct ggacactgtt cttttatatt cgatagtatt tcatcctgag
    61921 aaaatttgct catgtagatc aattgttttt agaactaaca attacataga tggctgtgct
    61981 atcatacgtc atctgctgag aagggcgggt ttcatctaac tgtgatccac ctttaccaac
    62041 ttcttttact acacttaatc caatagatgc gcgagaagta gaccaagaaa atccaagaga
    62101 gtaacctttc aaatttacat actcatttcg agtaggagat ctgagtgctg gaatttgata
    62161 attaatcaca ccattaggtg tctgtgcaac tacttctgca tcacttccta ggtctctatt
    62221 cgctccttca atggcacttc taacccaact aatatctttt gagttcgcat tgtttgtgaa
    62281 caaaccagct ctaattgcga aattatctgt taaaaaatat tccaaaccaa aagcaaaatt
    62341 taatgttgca taacgtctca attctcggac atcggtgtct ctaatcgtat actgattagc
    62401 acttcctcct aagaaataaa aatcattttg attcatccct ctttggtatc cagaggtgta
    62461 aatcgtatca aaagcagcca taagcttttt agtaggaaaa acagcaattc cagttctaac
    62521 ctctgtcact tctggaattc tgccatttaa ctttcctcca aaatatggtc tgcttccaat
    62581 acttccccca tttgcctcgt gggtgccctc ggtaaaataa attccatctg catttgtatt
    62641 ggattgataa gcaaacccac tcgtcagacg atttcccgca gtcacaaatt gatgacggac
    62701 agaaagacct aaggaaactg tgttatttgg cataaacata ataccaagaa caggcataaa
    62761 acctgtagtc tttcgattat cgcttccagt gataccatta aagtttctat tcgcaaactg
    62821 aactaatcct gtatttgtta gattggcagt atcatgagta taatagagag tagcccccac
    62881 agataatttt tctgtaatag atttggcgat actgaaacca gcttgcactt gattatatga
    62941 ttctatgtta taagatctaa ggcttctaac cgatggataa taaatcggat ttacaatctg
    63001 atcatttcga ttgaaggttt cgttcagagt atttaaaata gaaaaggcaa ctttgtaacc
    63061 ccctacttct ttcacgattc caaagaaatt tggaatatag ttttgagaac ttctatcata
    63121 tccttgtcct ggaccgatta cattttcata cgttttagat gttcgagtaa tattactagc
    63181 agataaggaa atggagttat cgtaagcaaa agttaaacca gctggattgt aatacgctcc
    63241 agatggatca tccgaaatag ctgtataagc tccacctaaa cccgctgcct tttctccaaa
    63301 gaatccattt atattatggt aggagtctgc atataaagaa acagtgcaga ataaaaatag
    63361 gataatgatt cgttttctta taatcaatgg ctctttcctt tatttagttt taaatttttg
    63421 gaatattgtc tttatagcgt ttaacgttaa ttttacttcc ttttggaagt ctctggtctg
    63481 aaataaattt ctgcattgca ttaattctat catctacagg agggtgagtt ttcacaaaca
    63541 cttgattgtc atttgccgat ttcatttttt taagataacc aatgtaagca gaagaatcat
    63601 aaccaagctc tgaaattaaa actgctgcgg atgcatcagc ctgaatttca aattctttct
    63661 ggcgaccatt ttcaagcata ctctcttcta gttctttaga agctgcctcc atgacgctag
    63721 ttaccatatc ccctcctgga gataagagag aagctaccat atctatccaa atctcttctt
    63781 tcgcttcata ctttccagaa tgaaaaagag atacatggga aacttcgtgc gaaagaactc
    63841 cgacaagctc tgcttcattt tcaatcaaat ttaatgcacc ctttgtgata aaaatatatc
    63901 cgccaggaca agcaaatgca tttacttcag gagtatttag aatgccaaac ttgaaattca
    63961 actcttgcct cggggaaagt tctgcaattt taccaccaat aaaattaata tactttgttt
    64021 gatttaagtc ttgcacaagt ccatacttct taataaattt agcagctaac gcacgcccaa
    64081 tttttaattc tttataaact tgtttaggag gatccggctt ttcattttta gtatttgcag
    64141 atttgcaata gaataaaaat aaaatcaaac taacgattat tgtgcttttc ataaaatata
    64201 cagcggttaa ctccgattac tcttctttaa atttttgaat atcttctggt ttcaaagtaa
    64261 cgtgatccag ccaatctaaa gactcaaaat catattcatc actattgccg cgtgttcgta
    64321 ttttttttga ttcagaaagt ccacgagcag aagccgtttc tgtaaaagtg gatgctcttg
    64381 ctcttgattg gataggacct gatttatctg tattacctgc attactcact ttgttagaag
    64441 gaggaatttt ggagacatat aatttcataa cccaaccagt atcgtttcct gattttactt
    64501 taagaaacat atcctgttca ctaatctgtt gcaactcttg tcctgcctgt agtggcttcc
    64561 ctgcacttgc aacgtttggc tctgacaata acttcgctat tggactctgc acataaacag
    64621 gattagaaaa aagagaatga gtaaacgata agaataatag aatgaataat ttctttggat
    64681 tcatattgat tccctgcatt ctcattttaa ttttcaaaaa tttattgcaa gcaattaata
    64741 ggaattttag atagaatcat ggaagcacaa gagaaattag caactgaatt taaattactc
    64801 acaaaagaat cagagaatat taaaaaaaga gcgagggaaa actacttgga acaagtatca
    64861 gatatttcag aaaaaataaa acaatttggt gatgaagcta gtgtaaaagc aaaacaagtg
    64921 attgataaag ttggcgacta cattaccgag aacccacaaa aggctactct tattggtgtt
    64981 ggtatcggcg ttggaatcgg tgttttagtt ggattattag cccgcagaaa agactagttt
    65041 ggcaaaaaaa aagaaaccca cttctcctcc aattgaagag aaagaagaaa aaaaagaaac
    65101 attactgagt caagtgctct tacttgtaga gcacttcgtt tcttatttcg agttactctt
    65161 tttatatatc cagaaaaagt ttcagcaaaa agttcaatat ggttttatta taaccatagc
    65221 gattctttat atgctgtttc taaaagtggt tggaagtatt ctaatcatac tggcttccta
    65281 ttttgccttg ctcaaattat ttaatgggga tcatattact acagcactaa cgcttggcat
    65341 cggtataatg atattatcct ttacactcct tgcaatactc attcgtaaga ttgtccaata
    65401 aatgaaatct aaaattccat tcaatccctt tgaaggaagt gaagaatttc gctccaaaaa
    65461 tcctaaagac atgagtctag acgaacttga gattctttta catgcaaaaa gactaaacct
    65521 aaggttagat gtggaacgac tccataaaca aatcaattat actaagcttt tatttgatat
    65581 tgtaagagcc tctggtcttg gggaaatgat cgaggatcta attacaccaa aagaaaaacc
    65641 agactcaaat caaatatgat tgagtcctat cgcaattaac gtaggacttt gtaaatttgc
    65701 atctttcaca gcttgcacag cacttcccaa agtagctttt actatttttt gattttgtct
    65761 tcctaaatta cttgctgcga taaaagaagt ttcttctgac tttccgcttt gtaatagatt
    65821 ggaaacagtc ttttccaatt cccctagccc cataaaaatt acatgtgcat ctgtatcagg
    65881 taaatttact ttttgaattg tcttatccca ataaccactt gaataagaaa tacttctagt
    65941 tagttctcta tgagtgagtg attctcccaa agcctgagat agcaattgag gactagaaat
    66001 tcccataatc aatttacaag caaaaccatt ttcctttaag atttggatct cttcagaact
    66061 tcgagcaaaa aaactaacgt ctccagcttt tagtcttacg actttcttac cggtctttac
    66121 ttctgctagc aataattcat tgatttcgga ctgttttttt acatggaacc cttttttctt
    66181 tccaacagat tcccatactg cttgcggata ataaggatta agcgcttcta gatacatatc
    66241 atacaaaatg agatctgctt gcgaaagaat agaatgcgcc tctattgtta aatgcctgat
    66301 atcaccagag ccagcaccta tgagataagc agtcttttcc tcaaatgaat attgggggag
    66361 attcatacac tcaaaccttt ctttttcatc tctctttctt ttgctttcac tcctacttgt
    66421 ctatctaaat tagaaagatc aatcttagat tcgatttcct cccgaatcat ctttgctcca
    66481 cccggagatt ttccataagt agaaatgaag agagtaaaat tctctcgaat caaagtagag
    66541 gcagatataa aatcagaatc ctcgacttga tcaataaaat taatgagaac ttttctttct
    66601 tttgcttctt gtaaaatttt cttttcctca atggggtcat tgattccaca gtaaacaata
    66661 tcaaatcctt ctaagtctcc atattgatac tttctataaa taaaatcaat ccaatccaaa
    66721 gaatgaaatt cttcaatgaa ctgcggagaa atcgctgtta tccgcagatt taactgtgca
    66781 agagttctaa gtttctccgc acttgccttt cccccaccca agagtaaaat ttttcgattt
    66841 gtaacatcca tcatgagtgg aagatatttt ttcagatgag gtctccttct attatcatac
    66901 aagcccctga ggtttggttt gttccttcat ctacaaggat gcaagagcca gttcctctat
    66961 tgactgaata ttcatcatag aaaattggtt tagcagtttt taaaacaatc tttgcaatat
    67021 catttaattc gagcgaatct atattttcaa ttttctcaag tgtattgatg ttgattctgt
    67081 attttatctc ttttactata catttcacag aattagtcgt atgacgtaat gtgtatttgc
    67141 cgccagtttg cattttctta gtatccatcc agcaaacata tgcttcaaat tcctgtgtct
    67201 cgactggaga cttatctttt acaagcatat caccccgact aacgtctatc tcatcttcta
    67261 atcggattgc aaccgacata ggaggataag cttcttctac ttctccttca aatgtatcaa
    67321 ttgctttgat tttggatttt gcaccagatg gaagtgctgt caccaaatca ccttttctaa
    67381 atacacctgc atttaccatt cctgcatagg ctctataatc atggtattca tttgcaattg
    67441 gacgaatgac ccattgaaca ggaaatcgag gtaagtcata gttatgcgat ttatcgatat
    67501 caatgctttc caaatattga atgagggagg gtcctttata ccatggcatg tttgttgagt
    67561 tttctaccac attatctcca ttgagcgccg agatcgggat aaagttaatt tctttaattc
    67621 ccattttctc tgttaaagga gaggcaaact ttaaatagtc tgctttgatt ttttcaaata
    67681 cttcttgtga atattccact aaatccaatt tattcacgca tactgtaata taaggaatat
    67741 ccaaaagagc gactaggaag gtatgtctat aagtttgctc aataacccct ttcctagaat
    67801 caattaaaat aatagctaag ttagcagttg acgcacccgt taccatgttt cgagtgtatt
    67861 gaatatgacc tggtgcatct gctatgatga attttctttt tggcgtggca aagtatttat
    67921 acgcaacgtc aatcgtaata ccctgctctc tttctgcctt aaggccatct gttaaaagag
    67981 ccaagtttgt ataaccatct ccccttctct cgcttgattt ttcaatcgca gccatttggt
    68041 catcgaagat ctgtttacta tcgtatagta atcttccgat caaagtgctt tttccatcat
    68101 ccacactacc tgctgtaata aaacgcagca tatccatatt catataagct tctgtattca
    68161 tatttctgcc tttaatttgg ggaaatcata tttatttttc gatttttatg tatgaactct
    68221 aaaaataccc ttctctcttt ctttcttcca tagcagcttc ggatctttta tcatctgccc
    68281 taccaccgcg ctcagtaaca cgagcagagg ctacttcttg aatgatttca gttacattag
    68341 atgcattcga aataactgcc ccggtgcatg tcatatcgcc taccgttcta tatcgaactt
    68401 gcatttcctt tacttcatca ctcggtcccg gtcttactac atccgaaaca ctgagaagca
    68461 tattatcata cataaaacaa tctcttttat gggtaaaata aatgcttgga atcgctaaat
    68521 cttcttttaa aatatattgc caaacatcca tttctgtcca attgcttata ggaaatacac
    68581 gaatgtgttc cccttgttgg atctttccat tatagagatt ccaaagttcg ggacgttgtt
    68641 tctttggttc ccattgacca aattcatctc taaaagaaaa aattctttct ttagctctcg
    68701 ctttttcttc atctcttcta gctccaccga aagcagcatc aaatttaaat tctgcaagtg
    68761 catctaaaag agtaatggtt tgtaatttat ttctacttgg aattcgccct gtttcttcta
    68821 ctgcttttcc ttgattaata gagtcttgca catatcgcac aattagtttt tcatcgattc
    68881 tctttaccag atcatctcta aatgtaattg tttctgggaa attgtgtccg gtatcaatat
    68941 gcagaagtgg aaatggaaat ttagctggtc gaaatgcttt ttctgcaagt cgcacaagtg
    69001 taatcgaatc cttcccgcct gaaaataaaa gaactggtct ttcaaactgg gcggcaactt
    69061 ctctcattat atagatagat tctgcctcta attcctttaa gtgattcaaa tggtatttac
    69121 tcaaactaaa actccttttt catcttagac aaacatggat ttgtcgcttt taggcttatt
    69181 tggaactaac ttcccgtctt tccagtgcaa tccacattct tttgtagcag gatcttccca
    69241 ccaccaacgc cctgccctag aatcttctcc ctcttcgatt gctcttgtgc aaggagcaca
    69301 tccaatacta ggatagttct tatcatgcaa ttcattgtat ggaacgttat tcgcacgaat
    69361 gtagtcccaa acttgttttt ctgtccaatt cagaagagga ttaatcttca taacagaata
    69421 tttttcatcc cattcagcaa acttcatatc ggaacgagta acagattgct cctttcgaag
    69481 tccagtaatc caaagagatt tatctttcat tgcacgagaa agaggttcta atttacgaac
    69541 agagcaacac tcttttctat tttcaatgct ttcatagaaa agattaatcc ctttttcttt
    69601 gaccattttc tcaacagaaa tataatcagg gaactgaact tgaattttga tcccatactt
    69661 tttctctgta tcttctatgg tcttatatgt ttcgtatggc aaacgacccg tatccagtgt
    69721 gactatcgta atggttttat caattgatag aattaggtct gttaaaactt gatcctctgc
    69781 cccgagacta gaagaaaata aaactccccc cttatgctta gccagtgcga gttgtaatat
    69841 ttccttcgta tttgaaattt gaaacttttg attaaactct tctaattgtt ccataaaata
    69901 tttttctcaa ttggattatt cacttttagc aagagtaatc ccaagcaatt ctgcaacctg
    69961 ttctttttcg taagacttat aaaaatcacg gaaggattga cccgcttctt tctttgcgta
    70021 atagtgatta gcgagctttt caacaatcgg tccacaatga tctgctttta cttttaatac
    70081 acttctagta aaagacggat tgtttccaat cgatccacct agatgaagca tataagcttc
    70141 ttccatttga tcgttttccc cttttgtctt acctcctata aaaccgatat ccgcaataga
    70201 gtattgagaa caactatttg tgcatcctga aaaatggata ttgatattat ctacattgat
    70261 tttcttctca ttcaaataat caatgagttt agaagttcta tgctttgtct caacgattgc
    70321 caggttacaa aactctgaac ccgtgcagga gacaattcta ctatgtgttt gcaatgggct
    70381 gtaaattcca gaatcctcta aagccttgac tgcatccttt actttctcgt ctggaacacc
    70441 taaaattaaa atgttttgct tatttgtatt tctgagtagt ccattgccat acttctcggc
    70501 tatgtcacac aacgttatca attggtctcc agagagtctt ccaactttta tcggaactcc
    70561 tataaaattg agtccaagtt gtttcatttt ttgaggaccc agtggatcag aatttctttc
    70621 taaaatttga ggatttggat aatctgtgag tttataacca agtttttttt ctaactcagc
    70681 tctcattttt tctggtcccc aatcttccat taaaaacttc atacgagact tacgacgatt
    70741 ctctctattt ccatggtcgc ggtaaagttc tgttagcccc gcgcatactt tcaatacttc
    70801 ttcatcatct tttaaaaata cattcagcca tttggaaaaa tatggattgg tcgaaagtcc
    70861 tcctccaaca cgaattgtat atccgtattt tgtttctcca ttgacttcta attttgctcc
    70921 tatgattccc atacaattga tttcaggttg tgctaaattg tgaggagcac ctgaaataga
    70981 aactttaaac tttcttggta aatcacctaa atccggtctt tcagcgattt ctttcacata
    71041 tcgattaatt aaattggttg catctaaata ttcttctgga tcgattccag cgagtggatt
    71101 acctgtaacg ttacgtgtaa tatctccaca agctcctaat acccctagcc caattttttc
    71161 gagggtatca atcataacag gtatatctgc tatcgtaagc cagtgaactt gaaagcattg
    71221 tcttgtagtt acatcgatta gatttctagc tctctctttt gtaagacgtg cgatttctct
    71281 accttggctt gcagtatatt gaccagacgg aattcgcata cgcatcataa aatacccatc
    71341 tgtcttcggt ttttgtaaat agcatccaaa ccatttaaag cgaatatggt catcttctgg
    71401 aattgcctga aagccctctt ttgcccattt atcaatttct ggtcttaatt ttaaagggtg
    71461 tttctctttt ttaatacgtt cttcatttgt gattttagat tcttcttttg ccaatttcaa
    71521 aaacctctac tcaaaatata tctttaccat atcaaaagta ttaaacatct tgtcaatata
    71581 ttagaatatt catctagtat aatggaaaag cactatttaa ttggtatata aaaagagtag
    71641 gaatcttgaa tttctctagt atttccaaat caattcttaa aacgtctgct tccttctgtg
    71701 aatgaaatta catttgacaa caagataaat tcccctataa attaaatcaa agagtgcagt
    71761 tctatcatct gaattcgttg aaagccatta tatctcagtt atattttaac agcaaattag
    71821 aattagccgc attcaagaca tcatagatca tatgcaaaca agtagttcac aaattaactt
    71881 tagaagtaag atatccgctt ttctacttag atatttctcc aaatttttct tcaaagatgt
    71941 taaatactac cgcgatccga atgattcaaa taatttaact cttccctatc catgcaccat
    72001 ctttataaac aatgtagaag aaagagatat actacttgtt ggatatctct cggaaatttt
    72061 caacttacaa gcaagacttt attttccaat gcaaagaaat gtaggaaaga gaaactttcc
    72121 ttatccttct aactcaaaat ggaaattatt ttgcaaatta ataattttga aactttctat
    72181 cataccgata ttactcaggc taatcggagg aaagtatgcg tcatacgata cttttaagct
    72241 tttaaaaaca atccgccgca atacatcttt ttctatctat gagaaatttg aatccttcaa
    72301 gcaacaaata tctatgcaaa aaaccttcgt tctcattgga catgaatcag agaatggcga
    72361 aaaggatatt gaaaatgtaa gtggaatact gagaatattt aataataaga atcctgatat
    72421 gtcttattta caaatcacat tgacttatga ctacttattt tctaaagagg ggatagtgca
    72481 tattagccac agtggacttt ttacaatgaa gcaagataag ccaatcgctt ctatccttgc
    72541 tacactccgt ttaaagagaa ctttaggaac tacggttact ccaggaaatt tatttgcatt
    72601 cgcaatctat tctccagaag tagaaaacgg tgtctctaga caatatttgt tttataaaat
    72661 ggaaagactt gcacatgtaa tctataaaga aaaattttgc aacattgcaa gggaatgttt
    72721 agaaatgtcc tatgatgatt tatttgcaga aattttgtat gacgcacgga caaatgactt
    72781 tctaaaatac caaacggact caaaacaata ctatggaaca aataaactat attattacaa
    72841 aaaaaataaa tactattaca ctgagctgaa gacagaaaat atttatagat accattatat
    72901 gcaactttat ctatttcata aaaaatttag acaaatctgg gaagagatcg aaatctacga
    72961 ttaaatttca ttgcctccaa tctttcgagt aagaacatag cttttaaaag tgagttgtta
    73021 tgaatattcc aaagatccaa ttacctagaa aagccaccta tacaagatta gaagaagata
    73081 ttcccttttt tgaactattt caaaagatag aacgcaaatt tgaaaattgt tttctactag
    73141 aatcattaga aaaagaaagc tatgattctc gttattccat tttaggtttt gatccgcaac
    73201 atattgtata tgctgatggc aattctcttt atgtagataa cgttgaatat ccatgcgaca
    73261 atccctacta cgctcttaga gagttaattc caaccgatgt gctatctgta aattatgctg
    73321 gaggactcgt tggatttatt tgttatgagg gaatgaacta ttttgaacct tctctgaatt
    73381 taaaaccaca caaggacttt catgctttcc actttggcat ctatctagat ggaattattt
    73441 tagataagat gactggtgaa actttttact tctattatga aaacaatcga attgagcgga
    73501 taatggagat tatccaggaa gaaaatataa gtgagaaatc ttataaaaca aatattgaat
    73561 tcttaggcta tacaatgtca gaggcagaac acaaaggcaa agtcttagaa gtattagaag
    73621 aagtaaaagc tggaaataca tttcaatgcc aactcggtct caaagcagaa tacaaaatta
    73681 caggagacac ttttccaatc tatcaagagt taagaaatgt aaatccttct ccccatatgt
    73741 attatttgaa attcggagaa aagaaaatca tcggagcaag tccagaatta ctcttccgac
    73801 ttcgaaatgg cgaaatggaa acctttccat tggctggatc aatcaaacg
//
LOCUS       DAOQOS010000008        72862 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_117_length_72862_cov_6.454448, whole genome shotgun sequence.
ACCESSION   DAOQOS010000008 DAOQOS010000000
VERSION     DAOQOS010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 72862)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 72862)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..72862
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117_length_72862_cov_6.454448"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            77..610
                     /locus_tag="PLF66_02910"
     CDS             77..610
                     /locus_tag="PLF66_02910"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HMZ63018.1"
                     /translation="MNLEKYHPTKVFRYCPRCGKESFHPSSERSNECSECGFVLYFNS
                     ASAVCALIFNEEGKILLTRRERDPGKGMLDLPGGFVDLNESAENALSRELKEELNLEI
                     SDPKFIASFPNEYLFKDLLYFTCDLAFVCKANFTGNMTPNDDVSEFLFVDLNQLDLNL
                     IAFESIRKVLRFYLNSN"
     gene            complement(835..2991)
                     /locus_tag="PLF66_02915"
     CDS             complement(835..2991)
                     /locus_tag="PLF66_02915"
                     /EC_number="3.1.4.35"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020772740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP/cGMP-dependent 3',5'-cyclic-AMP/GMP
                     phosphodiesterase"
                     /protein_id="HMZ63019.1"
                     /translation="MSGRDEKNPCLISLPRGGYLVKTDISSIQFGSPPETIKDTMFLE
                     GGVPQVFVLPNEFFSWTKGISVAELEFPIYYNFFFRRKKTFIICSEKQYRRFKEVLRE
                     SIFGPRNLDLTCDYELLNGNTYIPDIKSEHNFFSSTFKFQDMVGFGIFKDNQFTIHGT
                     TIRKKENGDFSVSIGANHVTDVPGKMEYKGFYAIGERLREPFIPPLFGVTCLGPSHGF
                     DPEENTSGFIIWLNHSGIMIDPPVNSTEWLLDSNVNPKFIDSIILTHCHADHDAGTFQ
                     KILEEGKITIYTTETIMKSFLYKYSTFTNVSQEYLKSLFTFQPIKINKPEFIYGGKFQ
                     MFYTLHSIPTIGFKMEYQDQSFVYSSDHNNDPEIHEKFLREGIISQERYEELRNFPWD
                     SNVIFHESGVPPLHTPIKYLDSLPEEVKKKTVVYHIAAKDFPKETSLTLAKFGIENTL
                     YFPMTPPRFEKASQILGVLKNLDLFEGLSISKAMEFINIVEEVTFKKGEIIINKGDSA
                     DKFYIIYFGNVSVVSEGLVSKKVYGTYDYFGEVALITDQKRTAAVIAETDVVVYTISK
                     DKFLSFISGTEFEKTLAKVARMRDAESWNVLSSSSFQALTATQKTLLESMMTPIEIDQ
                     PSILMQEGHIMEQIYIIRKGEVKVEQKGKLKGILKRGDYVGSLIRIRRGHPSEYTYIN
                     EDSVSLYKVSHDDVIRFVTRNPGLMMKLVYDFSNTR"
     gene            complement(3107..3628)
                     /locus_tag="PLF66_02920"
     CDS             complement(3107..3628)
                     /locus_tag="PLF66_02920"
                     /inference="COORDINATES: protein motif:HMM:NF017538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqiA/YcfP family alpha/beta fold hydrolase"
                     /protein_id="HMZ63020.1"
                     /translation="MVNSKNVTIYFSHGKESGPNGSKIQRLSKIAEAFGLEVKSIDYT
                     DTMDPEVRADRLIKILSDRKVDSEFILVGSSMGAYVSLVASNQFKPLGVFLLAPAVYI
                     YGFKEQKYPIIENLEIVHGWEDEVIPYELSIRYARESKSVLHLIQGDHRLNSSIETVE
                     GLFRNFLTRTLNR"
     gene            complement(3632..3964)
                     /locus_tag="PLF66_02925"
     CDS             complement(3632..3964)
                     /locus_tag="PLF66_02925"
                     /inference="COORDINATES: protein motif:HMM:NF025715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4404 family protein"
                     /protein_id="HMZ63021.1"
                     /translation="MIQDTITKIEENIRDSKSLDVAKKEELLGLVVSLKKEVVELSAT
                     NKEDAETVANFTKASTHEVIKSNKNKDLIDLSIKGLSESVSKFEASHPDLVSVVNSIC
                     VSLSNSGF"
     gene            complement(4109..4651)
                     /locus_tag="PLF66_02930"
     CDS             complement(4109..4651)
                     /locus_tag="PLF66_02930"
                     /inference="COORDINATES: protein motif:HMM:NF019235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1564 family protein"
                     /protein_id="HMZ63022.1"
                     /translation="MNHQSSFLKESPKLFSNQKFAFPRKDYIYSEFSSTILIPDQYHQ
                     LFWEKCLAHGGVRQYIAYLLSKYQLYIANGLVPGYANLTTKYQEKGQNLHKIAFRPRP
                     EDWAELKLYRVSFGMSISAFLVYLLIADSVDFAKASSHHLVAVGIPEISNFDLIAKIF
                     LMNNRLNYTVIFQYRKSRYG"
     gene            4728..4904
                     /locus_tag="PLF66_02935"
     CDS             4728..4904
                     /locus_tag="PLF66_02935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63023.1"
                     /translation="MSFVLFHDPLLSSHLVFKDSKVRPFKLFHQSHSVRMGIHVLYRS
                     VIGGDPLKEVGKDI"
     gene            5130..6521
                     /gene="leuC"
                     /locus_tag="PLF66_02940"
     CDS             5130..6521
                     /gene="leuC"
                     /locus_tag="PLF66_02940"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010419888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HMZ63024.1"
                     /translation="MKTMFEKIWNDHLVTENNGSAILYIDRHLIHEVTSPQAFESLRL
                     SNRKVRRPEATFATMDHNVSTRSRDMDFADPISAIQMKTLIKNCKENNITLYDLTNPD
                     QGIIHVIAPELGLTHPGMTIVCGDSHTSTHGAFGALAFGIGTSEVEHVLATQTLVQQR
                     PKTMEIRVEGKLLPHVTPKDIVLAIIGKIGTDGATGYVIEYTGEAIRSLSMEGRMTIC
                     NMSIEAGARAGFIAPDETTFNYIKGKDHAPKENFDLFVKKWKSYATDPGATFDKTVIL
                     KAEEISPMVTWGTSPGQVIGVTETVPNPESATDPTVKKSIEGALAYMGLKAGQKITDI
                     TLNKVFIGSCTNSRIEDLRIAASTVKGKKVSPKVNAIVVPGSGRVKRQAESEGLDKIF
                     IEAGFEWRNPGCSMCLAMNDDVLSPGDRCASTSNRNFEGRQGKGGRTHLVSPAMAAAA
                     AIEGKFVDIRYWS"
     gene            6523..7155
                     /gene="leuD"
                     /locus_tag="PLF66_02945"
     CDS             6523..7155
                     /gene="leuD"
                     /locus_tag="PLF66_02945"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004460150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HMZ63025.1"
                     /translation="MKAFTTLNGLAAILDKANVDTDQIIPKQFLRKIERSGFGVHLFH
                     DWRFLDDAGTKPNPEFILNQPRYQGASILLARDNFGCGSSREHAPWALEDYGFRCIIA
                     PSFADIFYNNCFKNGMLPIVLPSEQVEELFQYVEKNPNAKLEVDLMNQVVKAANGKSY
                     SFTVDAFRKDCLYKGLDDIGLTLVHENSITNFEKARRSKYRKCLRENKKK"
     gene            7291..7683
                     /locus_tag="PLF66_02950"
     CDS             7291..7683
                     /locus_tag="PLF66_02950"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002747878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HMZ63026.1"
                     /translation="MFKILLAISLFAFASVNVNAQEVKDLIVKDVKVGNGKEAKDGNT
                     VTVHYVGTLTNGKKFDSSKDRGQPFSFQLGRGEVIKGWDRGVKGMKVGGTRKLTIPAH
                     LGYGDRGAGSDIPPGATLLFDVELLKVD"
     gene            7836..8672
                     /locus_tag="PLF66_02955"
     CDS             7836..8672
                     /locus_tag="PLF66_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016547237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HMZ63027.1"
                     /translation="MSKEIYTESELNLFKKSQKLAYNSVLEAESKLTEGITEKEAAHL
                     LDEILKEKGVKNFFHTSFAWFGDRSCFRGFTNYFDFQPTNRKLEKGMSVILDTGPSLD
                     GRACDIGYSFSFGENSLVDKAILDLEVFRSFILKEVLAERSLSDIYLEVDKIIKEMGY
                     FNCHKKYPAEVLGHKIGNLPFLQIPPINFFGFHSQTFLYLLGETANALLPNFLLQGNN
                     TYPFWNHSSNTRAESGLWAVEPHIGKEDVGVKWEEILVVTESTAYWLDEELPHVSLWK
                     KK"
     gene            8739..9107
                     /locus_tag="PLF66_02960"
     CDS             8739..9107
                     /locus_tag="PLF66_02960"
                     /inference="COORDINATES: protein motif:HMM:NF013035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine rich repeat-containing protein"
                     /protein_id="HMZ63028.1"
                     /translation="MNKIFLFAILALTLTFSSFAQTAEEDPKPCKEDRQTLCKDVKPG
                     QGRIIKCLKENEAKLSPACKTHLEEKTAEAKENHNKCKEDRKKFCKDVKPGEGRIIKC
                     LKDNEAQLSADCKAVMAEKK"
     gene            complement(9378..10529)
                     /locus_tag="PLF66_02965"
     CDS             complement(9378..10529)
                     /locus_tag="PLF66_02965"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HMZ63029.1"
                     /translation="MKKNKSIFLIILLAVNSILPQASPVNEPAKKTEQAVETKKEVGI
                     SFVNSEWKDVVAKATKEKKFIFIDAFTTWCSPCKWMDENVYNSKKAGDYFSEKFVSIK
                     LNMEKEEGLSFAEKYNVHAYPTYLFFDSTGKIIHRFQGPLEVDDFLKEVGNTFIPEKA
                     FYELKRKYEKGAKDDKTLYELVFASYKAFGEVNGDLLEDYFDTQDDLISKKNWKIIDD
                     FVTDIKSKTFKYLTANREKFYPSHGKDNVEKKIYLTKMAYYSKAEDWKNYATVAIAYA
                     EKYSMNEWKDLDAIAWRISQKLKDPKTLSKAEVLIRKSIQLEPNFLNHETLAQVLYKQ
                     GKYKDAHLVTMNSIRMAKEAGESYENSISLLNLIIQKLLLQIEKDKKGK"
     gene            complement(10539..12434)
                     /locus_tag="PLF66_02970"
     CDS             complement(10539..12434)
                     /locus_tag="PLF66_02970"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HMZ63030.1"
                     /translation="MNKKVGIKERIKYEFENTLSKGTIAIIGWLAFGSLLIVILAGIV
                     LTFGQISQNPEEKLNFFEAFWQSLMHALDAGALGADQGWSFRMVMLLVTIGGIFILSS
                     LIGVLTSGLEAKLEELRKGRSRVLEKNHTLILGWSGKIYPIISEIIIANASLKKASIV
                     ILADKDKVEMEDELNSKIPDRKTTKIICRTGSPMDVNDLEVVSLDDAKSIIVMSPDDE
                     NPDISVIKTILAITNNPHRKQGKYHIVAEMKHQENLAAGALVGGDEATFVLSPDLIAR
                     VTAQTCRQSGLSVVYTELLDFDGVEIYFKEEATLKGKTFKEILFSYETSAVIGIMRSS
                     GEVLINPPMDTVFAEKDSIIVIAEDDDAIDLAEKSNYDIKSNLFQSPKPEGLKQERTL
                     ILGWNEKGYRIINELENYVGNGSEVTIVSELSETEEEVKELELKKQKVTVKQANITDR
                     NVLDSLEVSTYSHIIILCYTNKIDIQEADAKTLICLLHLRDIANKTGKNLSIVSEMLD
                     NKNRALAEVTKADDYIVSDRIISLMLSQLSEKKELKKVFDILFEAEGSEVYLKPVTDY
                     VKTGDPMNFYTILESAAEKNQIAIGYRIHANANDSEKAYGVLINPKKSDLIRFNAEDK
                     IVVLAED"
     gene            complement(12522..13526)
                     /locus_tag="PLF66_02975"
     CDS             complement(12522..13526)
                     /locus_tag="PLF66_02975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63031.1"
                     /translation="MNFIRFLFLIFTLLLVAFCKKENHELFGNETSSKSFWTTSKIVL
                     SPAIREEKFYKLFKGYQKNFHFEGSGVYARGNQLYVIFDNMKAVGVIEASLENNQMNF
                     LKGNIFEKSNYEAITYKDENPFQFYVMIESKEITNGYYPFLVRLDADLNFQEEKIVKF
                     ELEKKNKGIEGISHVSRNGENYILALCEGNFCNSDKEKHRGNGRILVLKEEKDHWEIT
                     AEIKLPSSISFLDYSDMDIENGRVAIVSQMSSALWVGELHSEKWEVLEKGTLYYFPFG
                     DREGNAGLGGEIIYCNIEGVSWLGHHEIVVVSDKRNEDQDKWCKHKESMIHIFEISNH
                     "
     gene            13570..14274
                     /locus_tag="PLF66_02980"
     CDS             13570..14274
                     /locus_tag="PLF66_02980"
                     /inference="COORDINATES: protein motif:HMM:NF027640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63032.1"
                     /translation="MLYKTGIISLYSQMDFDFLKLILQAPEKQDMVPMLELAFRIGVA
                     TLLGFLISGVSYLTYTGKNYDRSVIHSQIIVTIVFSVMINVIGQNLAWAVGIFGSLSF
                     IQFRTTLRDAKDTATFFYSVVTGVACGAGYINLAIFGFFVMSLVQVILKYLPPLEVHY
                     TYIKFNCKNIEAKEMIKSFLKRERVKYELAAISVKAENVIFAVRLPMESSYELAKKAK
                     LKLPEWVDSFSLDREL"
     gene            14472..14813
                     /locus_tag="PLF66_02985"
     CDS             14472..14813
                     /locus_tag="PLF66_02985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63033.1"
                     /translation="MKKTILYIGLSILSLNLFVLFADSANAISLGQVSAVTDKTVNLS
                     ASGTPAPGAPKASSIKKGDILVILNEDNSVGEVKVTKTMQTMGGKKVLIEAEILSKSM
                     PIKTGMGFRIK"
     gene            15110..17014
                     /locus_tag="PLF66_02990"
     CDS             15110..17014
                     /locus_tag="PLF66_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002975269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA synthetase"
                     /protein_id="HMZ63034.1"
                     /translation="MGKYKDLFEESIQNPQKFWKEQLEIVKWFKEPETILSKDKDGLY
                     RWFEGGKLNTSYLALDYHIENGRGNDIALIYDSPVTNQIKKFTYIQLREEVAKFAGVL
                     KHFSLTKGDTAIIYMPMIPEAVIAMLACARLGVVHSVVFGGFAPHELAVRIDDSKPKL
                     MITASYGVEIHKIIPYKPMLDEAIHQAIHKPEHVVLYQRPDMEDLMHTGRDHYWHELM
                     ENVEPSGYEELDATDPLYILYTSGTTGKPKGVVRDNGGHAVAMKLSMEAVYDVKPRDV
                     FWAASDVGWVVGHSYIVYAPLLYGCTTILYEGKPVKTPDAGAFWRVISEHKVNVLFTA
                     PTALRAIRKEDPNGELKSKYDLRSLKYLFLAGERTDPPTYEWAKDLLEIPVIDHWWQT
                     ETGYAICGNLMGVEPVISKAGSATRPMFGFDVKILDEEGSEVKRGQKGNIVIKLPLPP
                     GCLPTLWKDSENFKKSYLSHYEGYYLTGDGGYFDEDGYLFIMGRIDDVINVAGHRLST
                     GEMEEILASHPTVAECAVIGIEDAMKGQVPIGFVVLKDGAKQDLAEIEKELIHLIREE
                     IGAIATFKNVLLVKRLPKTRSGKILRKTMRKIADGEEYTVPSTIDDPTILDEIIEYLR
                     EAKLGKAFQK"
     gene            17041..18210
                     /locus_tag="PLF66_02995"
     CDS             17041..18210
                     /locus_tag="PLF66_02995"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HMZ63035.1"
                     /translation="MNRWFYVLICGLINVCIGNVYAWSVFRKPAEKFFSITSKESGLP
                     FMIFLAFFAVSMPLTGKFLDKYSPRFFALVGGTVFSIGYILSGFMENIQQVVLTYGVI
                     SGLGVGVIYGTTIAVPAKWVPERKGLAVGLSILGFGVSPLITSYLSHSMIESYGLMNT
                     FKILGLVYMVIILILSTFLKFPNDTQQQALAQQLDSSSISPGAIVKGKYFYALWICFF
                     IGAFTGLSTISISSPFLIESTGIAAGEAALYVSFLAIFNGLGRPFFGFLTDKIGFPKS
                     ILVSYFVIILSSIILFLNFNHLIFLYVLALALLWSSLGGWLAIAPTATASLFGTKYYS
                     SNYGLIFTAYGTSALLGVYLSSSIRDLFGQYLSFFYPVIGLCILGILISLFTFKK"
     gene            18207..19730
                     /locus_tag="PLF66_03000"
     CDS             18207..19730
                     /locus_tag="PLF66_03000"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HMZ63036.1"
                     /translation="MKPLLTKILNVSQIRSLDAFTIENEPVASIDLMERASIAFVKWF
                     TAHFDSEFSIRIFVGPGNNGGDGLAIGRLLSQEKYKVEIYIPTYDQDRSKDFLINYNR
                     LPHNVKRVEINSTKEIEPAKNNTIIIDALLGSGLNRKPKGIIEDVISYINQSGFIIIS
                     VDIASGLFADKHTDHSSIVNPNYTISFQIPKLAFFFPDNSKYVGNWQLVDIGLSKKFI
                     EEEKTDSYFLNKNFLKSIYKRRDPFAHKGNYGKALLVSGSYGKMGAAILAARGCLRSG
                     VGLLTVQVPKKGVEILQISVPEAMVVSGKRKKTISEIDWKEFDIDAIAIGPGIGKEDQ
                     TLKTLTSLLTEVKKPMVLDADALNLISENPKLLDLIQPNTILTPHFKEFERLVGKSEN
                     DFHRISLLRNFAKDKKIFIVLKGKHTAIATPEGDVYFNGTGNPGMATGGSGDVLTGVI
                     LSLLAQGYEPLHASLFGVYLHGLAGDIAAKKFGEESLLPSDLINYLGNAFLQINNSY"
     gene            complement(19868..20626)
                     /locus_tag="PLF66_03005"
     CDS             complement(19868..20626)
                     /locus_tag="PLF66_03005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63037.1"
                     /translation="MKWIVPIVLVLALIFVDCKKKEEEKTTEEPVASVDSDPIAEEEG
                     IPRREEIANPTIPSPRGLRNLFEVRGDLDGDKEEELVIIYETSKDSDLGFEREIRIFK
                     KKDEKWELLYKNIDAILPSRAGGMLGDPFEDVKVENNAIVVNHFGGAREKWHYTHRYR
                     FQKDGWFLIGATTSFFAPCEISNTYDYNLSTGKVNVSNEIQACDENGEPKGKPKTKTT
                     TITLPKTDPISMDGFTPGNTEVKIPNTKDQSFYF"
     gene            complement(21069..21656)
                     /locus_tag="PLF66_03010"
     CDS             complement(21069..21656)
                     /locus_tag="PLF66_03010"
                     /inference="COORDINATES: protein motif:HMM:NF024919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="papain-like cysteine protease family protein"
                     /protein_id="HMZ63038.1"
                     /translation="MLRIILSFTLILPLLAFEKQEGDFILENLNPPKEYWEIPEGSCG
                     EACIYSIAKSLKMKITQEEINSLAGSPGRGIHSNEVLKVLKKLKIPFQNISTTVNNHR
                     KYLEEKVIQNVKKGNPILLGVKIYPDENPKWVCDHFILIVGYNEKTGELIFNSNEERG
                     RIQINKLLNKKDGFSILHKSKYVYAVQIVLAKNES"
     gene            21725..22522
                     /locus_tag="PLF66_03015"
     CDS             21725..22522
                     /locus_tag="PLF66_03015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63039.1"
                     /translation="MKFKIFLLVFLCLSNLSAEELPESIPNTLSIEYYQSLGHAGRGF
                     SMELKENNCKYSDKFINTSNDFSFQLSKEDWKELYHILRKNKVHKITTSSEMIHDYDG
                     LSLSIQWENKSIQISQSGSRISSNWEKEWNAITSHLEKIRSNEYQKAVKSVEISLDKS
                     LVGNFIHIQANSQGVVFRKYVEIKNISEDKIQLKFLPGKHRIYAIQYEGYDPKKFEEA
                     KANSMYPDNALNPYLETLKANHFTLTIETIQKGSFIIRMKDKKLVVN"
     gene            complement(22519..22926)
                     /locus_tag="PLF66_03020"
     CDS             complement(22519..22926)
                     /locus_tag="PLF66_03020"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HMZ63040.1"
                     /translation="MSFVYLLDTNILSEQLKPKPDLDVIEFLEKNEDVIATCSPALHE
                     LQFGMESLEDGKKKKVLESYIHTIRDTIPIFDYTPEASVWHAKERAKLIKAGKTPSFV
                     DGMIASIAHTQSLKLVTKNVKDFKMYKVEVVSF"
     gene            complement(22927..23217)
                     /locus_tag="PLF66_03025"
     CDS             complement(22927..23217)
                     /locus_tag="PLF66_03025"
                     /inference="COORDINATES: protein motif:HMM:NF014643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HMZ63041.1"
                     /translation="MVLLTEASYSIAHTKNNLPVLVHSLENQSVISITRRGKVVAYLL
                     STREYELLKGKRKSFLSACKQLRESEEFKKVSFSEKDFANLRDKTTGREFKF"
     gene            23331..24086
                     /locus_tag="PLF66_03030"
     CDS             23331..24086
                     /locus_tag="PLF66_03030"
                     /inference="COORDINATES: protein motif:HMM:NF017551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NRDE family protein"
                     /protein_id="HMZ63042.1"
                     /translation="MCILFIAINEHKNFPLIILANRDEFQNRPTRSAQFWEDHPNLLA
                     GRDEQDKGTWLGITKEKRISALTNYRNIPLHKEGRISRGLLVRDFLISNKTAEEYLEI
                     LESSKENYNPYNILLGNPTELYVYNNVSNQRQKLEKGFHGLSNAFLNSPWPKLSRGIE
                     HLKKAVLTNQLEKDYLFSLMQDETKATDDLLPDTGVGLDKERFLSSIFLKSEVYGTRS
                     TAILLFDRQNTVSFYEKTYNPKGEVVKEFSASL"
     gene            24244..24885
                     /locus_tag="PLF66_03035"
     CDS             24244..24885
                     /locus_tag="PLF66_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002773856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HMZ63043.1"
                     /translation="MKVGILGSGVVGQTLAKGFLKHGYEVMIGSRDVSKLSDFVKENS
                     NIKSGNFSETASFGDIIVLTVKGNVAIDAINLAGVKNLEGKTIIDTMNPIGDKPPVNG
                     VLNFFTDLNQSLMEKLQTSFPNLKFVKAFSCIGAPFMVNPNFSGGKPSMFICGNDENA
                     KKDVSKILDQFGFETEDMGKVEAARAIEPLCILWCIPGMSQNKWAHAFKLLKL"
     gene            24911..25966
                     /locus_tag="PLF66_03040"
     CDS             24911..25966
                     /locus_tag="PLF66_03040"
                     /inference="COORDINATES: protein motif:HMM:NF021285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HMZ63044.1"
                     /translation="MNSLLDTLYSTVVNRDLFFQSGWGDKHGLEYLLSVNPTSIHLRP
                     VKEIDIKWGEIKKEKEYLVQDGEFQSPFEFIYDIKGEKVKVELPEETKTAYIQRILPF
                     DFSLQSPLTIHFAATGDEGYSRRRLMLAIPLAKKGIGSVLLEDPYYGKRRPKGQKGVV
                     IRTFTEFLRMSRAATDEGIALLKYFHRAGHRKLGVTGISMGGYVSLTASARSNLEIAV
                     AACIPSHSGSPVYVEGALSKACDWTSLSSPFQNPIDAKHFMRLILDQSDIRSFPKPKR
                     MDAVIIVGAKKDGYIPNYSTQIIKDHWEKAELRWVKAGHVGSFLFCTPDFRKAIEDSF
                     SRLVSNEFYSNPIVTNL"
     gene            26581..28125
                     /locus_tag="PLF66_03045"
     CDS             26581..28125
                     /locus_tag="PLF66_03045"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012388078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
                     /protein_id="HMZ63045.1"
                     /translation="MIIEHSLDNSKITEDEEKKKVKTKSDVILIGAGIMSATLGVFLK
                     ELEPDLTITVLERLDNAARESSNAWNNAGTGHSAFCELNYTLENEDGSIDIKKALKIA
                     SAFEVSKEFWSYLVKEKKIIEPEQFIHAIPHYSFVWGEEDVDFLKRRYDAMIKEPIFK
                     DLEYSEERKVLFKWMPLVLKGRDPMAKVAGTKMAVGVDVDFGTLTRAMFKYLSTLEGV
                     TVKYHEDVKDLVREKDGLWNLTATNQLTHEIEHHEASYVFIGAGGGALPLLEKSEIPE
                     AKGFGGFPISGQWLRCVNPEVIDTHFAKVYGKASVGSPPMSVPHLDTRIINGKKELLF
                     GPYAGFTTRFLKKGSYWDLAKSLELGNIFPMISAGMHNLPLTKYLITQALQSHRDRMK
                     SLKAYFPKAKSKDWKLEIAGKRVQVIKADEKEGGVLEFGTEVVAANDGTLAALLGASP
                     GASTSVAIMLEVLADCFTRKTATPEWQAKLKKMIPSFGESFIQNEELVKRSRSMTEEI
                     LKLKLD"
     gene            complement(28177..28464)
                     /gene="raiA"
                     /locus_tag="PLF66_03050"
     CDS             complement(28177..28464)
                     /gene="raiA"
                     /locus_tag="PLF66_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004750477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HMZ63046.1"
                     /translation="MKIKFTWKHLDRSEAVESYANDKLSRLSKYVHEVTKAELSFEKV
                     HGNITAVFNMHADKADFNAHNTDKEIHACIDGIEDKLEHQLSHHHDKRAKH"
     gene            complement(28771..30768)
                     /gene="tgt"
                     /locus_tag="PLF66_03055"
     CDS             complement(28771..30768)
                     /gene="tgt"
                     /locus_tag="PLF66_03055"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002770823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HMZ63047.1"
                     /translation="MSRLNFQLEKEAENSKARAARFMTLHGEVLTPIFMPVGTQATVK
                     SQTKDTLETTGSNILLANTYHLLLRPGIEVFQRIGGIHKFMNWTKPVLTDSGGFQIFS
                     LENTRSITEEGAVFRSYIDGRNILLSPELSIETQKAIGSDIMMVLDECVPSTSDYNMA
                     REAMELTHRWAKRSLVARGDSNQALFGIIQGACFTDLRKESAAQITDMPFDGFAIGGL
                     AVGETKEERNDFCELSASLLPKNMPRYLMGVGTPIDLLEAVHRGVDMFDCILPVEQAQ
                     RSVAFTSLGKLQMRRGKYKLDDTVLDPSCDCVCCKNYSRAYLHHLYKANEFLGWHLMA
                     IHNLTYYHRLMASMREAIFKNTFLSFYRTKKVELLQSDSVNPPDIKISSLDKKKQLGD
                     YEIIQNKEGFWSVRQKSSGEVMHSVSDPKEEAKRLYIDQSNLKERLMDISHEDPLVIW
                     DVGLGAGHNAMAVLSCFESIHSARKVELISFEIDLDPLKLALRNSGRFPHLFHKAPKK
                     ILEEKFWEKDLFTWKLIHGDFRKEFSKEKIPDLIFYDPFSFKVDDILWSSEVFSEIFQ
                     YTYKPNQITRLYTYSASTAIRSALLYSGFWVGRGVGTGPKEETTIAYTNLPDLEIERE
                     KLLGRDWLNRREKSYARYPKNITESDKKIYDDKILSHRQFH"
     gene            complement(30777..31124)
                     /locus_tag="PLF66_03060"
     CDS             complement(30777..31124)
                     /locus_tag="PLF66_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010577046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HMZ63048.1"
                     /translation="MKKVITKNKSSREVTASILRGLNQAVAHAKGNKKQSVEHRFSIL
                     PLANYQKEEIKIIRKKINLSQNLFAKALGVSKKTVEAWESGRNIPQGPAQRILYIMKT
                     NPVLFHELKIISR"
     gene            complement(31139..31483)
                     /locus_tag="PLF66_03065"
     CDS             complement(31139..31483)
                     /locus_tag="PLF66_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HMZ63049.1"
                     /translation="MTRVFVSLKEFDTLWKSIGLDDGDLREVETFLITNPKYGKVISG
                     TGGLRKMRWALPGKGKSGGIRILYVDYPLHEVLYFISLLKKNETENISAEDKRTISKI
                     IETIDNVLKKTQ"
     gene            31595..33091
                     /locus_tag="PLF66_03070"
     CDS             31595..33091
                     /locus_tag="PLF66_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002978207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HMZ63050.1"
                     /translation="MIKVSNVYKSFGSQELFEDLSFAVNRGEKVGLVGRNGHGKSTLF
                     QMLLGNLEPDKGVITVPKNYKIGHLQQHLKFTKPTVLEECALGLPEGEEYETWQVERI
                     LSGLGFSEKDMERSPDEFSGGYQIRMNLAKLLVSRPDMLMLDEPNNYLDIVTIRWLEE
                     FLREWEGEIILVTHDRSFMDAVVSHVVAIHRKKAIKTEGDTEKLYNQINQAEEIYEKT
                     RLNEAKKRKQEEVFIARFKAKASFASRTQSRVKKLEKQGEMKALEAIETLELYFNAAP
                     FSASQMLSADEIKFSYTGNPPYLVENFSISVGKQDRICIIGKNGKGKSTLLKLLAGEL
                     EPLSGKINKHPVLKEGYFGQTNKLNMEESNTVVEEIKSADKSCTENLARSIAGGLMFS
                     EDAALKKIKVLSGGEKSRVLLGKILVTPCNLLFLDEPTNHLDMQSCDSLIEAIDQFDG
                     SVIMVTHNEMHLRAVATKLIVFDKDKISVYDGGYDDFLSDVGWSDESL"
     gene            33136..33384
                     /locus_tag="PLF66_03075"
     CDS             33136..33384
                     /locus_tag="PLF66_03075"
                     /inference="COORDINATES: protein motif:HMM:NF013561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HMZ63051.1"
                     /translation="MLALRLPIAIEEKLDEISKAEKKTKSQLLREAIELYFDAYQKKT
                     SAFELGKEFFGKYGSGVKDNSENYKKRLKERWNAKRSS"
     gene            33365..33769
                     /locus_tag="PLF66_03080"
     CDS             33365..33769
                     /locus_tag="PLF66_03080"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HMZ63052.1"
                     /translation="MQSALLDAGPLIALFDNSDKHHKKVYHFFQSYVGNLYTTWPVIT
                     ETMHFLNFNPNVQSDFLQWLQRDPLHIPEHNKAILEKLRKLILKYKNVPMDLADASLV
                     LLSEEMNIKHIITLDSDYAIYKMNRKSFKSLL"
     gene            complement(33766..34896)
                     /locus_tag="PLF66_03085"
     CDS             complement(33766..34896)
                     /locus_tag="PLF66_03085"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HMZ63053.1"
                     /translation="MDSNFKKTLSNNGSNFSNLESINRHFDLDYLKAMATNILDPLDR
                     YYFRSRMINFETIPERNNPEAPAIYICNHSGMGFPWDAIIFAGRVFQQNKFQMKNSIR
                     ALTAPALSASRYMQPYFIENFWKRLGGVDATLDNFDTMMQIKDANVLIFPEGIAGIGK
                     GFDKKYQLQQFSSSFIRMAIKYKTDIIPVSIVNAEYINPYSYKNDDLNKLVNKLGIPF
                     LPMGPLTPLVALQPWVYYFGMPSKLTYYRGKTIKLYEMTDKPLEKLKKKEIHALRDSV
                     QFQMQTELDLAVEIYGKDPYMLEELGDIWRDNLDKILYILPSGWPVLFQELERRYKSE
                     TNFKIPLDNASYLEALSKTPENIFYGMPILGIAGLLKWQGIF"
     gene            complement(34900..35271)
                     /locus_tag="PLF66_03090"
     CDS             complement(34900..35271)
                     /locus_tag="PLF66_03090"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HMZ63054.1"
                     /translation="MPQEFSEKRKAQRLTLEAFCDFKNTKGEQEKDCYIQNLGTGGMM
                     VLSVLPHKMGDQVTIHFQVKDKNFERVGVVKTAKPLSENRKHYLKIGKNLHFSNILNI
                     AFKEEISQNDFEYIKFNYLKD"
     gene            35537..36220
                     /locus_tag="PLF66_03095"
     CDS             35537..36220
                     /locus_tag="PLF66_03095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63055.1"
                     /translation="MKHLLLTVPFLILSLFCSSVQKVDPKADLVNGFTPIKNDELAKR
                     YAPLLKAGSIYGDPFALYYRASKDDAGNTHITYHFAWEKEENTTSGIGPFFSRNIYTG
                     GLKLQKTMFGKGDLEMVSLIIDSNGVIQQVEYEIAENYDPSKFGVKHKHVIRTEPHKE
                     LLAFEVMSWNHLFQYLGDKNESSQNFLQLTPTYFTQSLWEEYTMIKEKEGFFSRNRAH
                     KPYEVEYIE"
     gene            36229..36993
                     /gene="recO"
                     /locus_tag="PLF66_03100"
     CDS             36229..36993
                     /gene="recO"
                     /locus_tag="PLF66_03100"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HMZ63056.1"
                     /translation="MSLLKEKGIVVATRQVGEADAVITILGESGTKAKYKLKGIKKSK
                     NRPILASELGALIAIDYYQHKNEEIHNIKEVSLIERFEKIKETYEGYLLITYFCELMD
                     TILPNGDSHEKCFELLNAAFHAINEENFQPLILPFFKLKLLSVLGLVSRELICHSCEE
                     DMLLKNAASINSIHLDITCGDCMPPSKNQISIIKLMDRIFKSRYATLSKEETPISLIS
                     QLDQILNDYLRAYLNVTLKTFDLFYKSLGGKYEIRN"
     gene            36977..37192
                     /locus_tag="PLF66_03105"
     CDS             36977..37192
                     /locus_tag="PLF66_03105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63057.1"
                     /translation="MKFAIKTAIALAMISLILGFLLRYADRNEYEILKPEVINTEDDR
                     SSWAIIMDVKKDIFKNIEMNWQKKHTK"
     gene            37189..38940
                     /gene="argS"
                     /locus_tag="PLF66_03110"
     CDS             37189..38940
                     /gene="argS"
                     /locus_tag="PLF66_03110"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004476510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HMZ63058.1"
                     /translation="MKENEILKNLVLKELETAVSKFVSESDKDKLKIRVEYSRNEKFG
                     DYSTPFLMENKDLLGDPKANSDKFLNLFKDVKLFSKVDFTPPGFINFKIEDQFLLNYI
                     DSFLTAANGPFAQVEEKEKIIFEFVSANPTGPLNIVSARAAATGDTICNLLSTLGHTV
                     HREFYVNDYGNQVFLLGVSCLSRLRENLSGETLTIQEEGDTTPIQTLLEKNILPSEGY
                     RGEYIKDVALKIYAESDKKSTIDSLLKSKEYEKLAEKFSVWAVEKNLEAQKEDLVSFG
                     VNFDLYYSEKSLHEANKVLAVLEDLKKANDIKEEEGKQVFTSIKYGDDKDRVVVRDDG
                     RPTYLLADIAYHKSKMDRGFTQIINIWGPDHHGYIARLRGAMISLGFAESRFKVLIAQ
                     QVNLISKGEKLKMSKRLGQFQTMKDLLEFLGESSKDVGRYFFVMRSLESPLDFDLDLA
                     KEESDKNPVFYIQYAHARIHSIFRETGNEYSFEKLKSISLTEERRSLLFWVSRFPEEI
                     LDAAKNREPHRVTNYLQNLSKAFTRFYGAKDNRLKDCDESTRLGLAYICQASAKAIKE
                     GLSILGISSPEKMERSA"
     gene            38940..40184
                     /locus_tag="PLF66_03115"
     CDS             38940..40184
                     /locus_tag="PLF66_03115"
                     /inference="COORDINATES: protein motif:HMM:NF013183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidohydrolase family
                     protein"
                     /protein_id="HMZ63059.1"
                     /translation="MLSIHIISTGTEITSGKSVDTNSTWIANELTGLGFSINKFLALP
                     DKPLLIEEEIRSIMARPGENLIIMTGGLGATDDDHTLNVVCKIVEKDAVTDQKALDKL
                     TYFSEQRGKMYQDLLPVSKRQTTIPLGARPLDNDIGLAPGFYLELNTTTRLAAMPGVP
                     KEMKKMFSDYFLPLMKKDYNKEDLQSRSRSIWGVTESIFQATFIKKHQELINEGLEWG
                     VTAKPGHIKVTFKSTSSERLRKIISFLDTEYKEKIGDEIFLDIHSMLISSKRTVSTAE
                     SCTGGLVGKILTDMSGSSAYYLGSVVAYHNDIKQNILGVKKSTLDTVGAVSSETAIEM
                     AEGVQHKFNTNYAISVTGIAGPTGATKDKKVGLVYIGIKANHEETKVFRYEIPLSRDI
                     FRDYVANIALFHLYQKLKQDNL"
     gene            40189..41256
                     /locus_tag="PLF66_03120"
     CDS             40189..41256
                     /locus_tag="PLF66_03120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63060.1"
                     /translation="MLYYFFFTIILSFIIFLNLGTPYTQMSHVQENWAWNKSQEIGMS
                     QDSRFKINPLQTLNGYKTKNGVYFFKAGKFSLEENTYSELPLNGKGHFVYKKIGSSVF
                     YYSNDGEILWEKPYKSYPRVSNNGELLLFISGDGNQVLISDINGNEFGAKQIDGRFLS
                     DISFPALVNGALLVFSGGEIALLNGSGKIVFQKKDEPNSKDFLFCKSGAISPTGKFSL
                     VHYIRNTKDFISILNEKGEIVSSITLESVYPHKIFMAVDDEGNMAVNLPDQVFVINKK
                     GKSVFKKVKSRKEDIYQIAYTSGSFFAVSLENQILFLKSDGSYLGKRSVTMPARMRPS
                     TERDSAFLETEEEIISFRLFK"
     gene            41266..41931
                     /locus_tag="PLF66_03125"
     CDS             41266..41931
                     /locus_tag="PLF66_03125"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HMZ63061.1"
                     /translation="MIVEPKDLVPFACKATGKCCIHNLVLLSSFDIFRLAKSLSLTAK
                     DLFEKKYLTYRINPVTYWMQPILNEKSNSTCPFLVRDIDSTNEKYICDVYEARPLVCR
                     LYPLKFDKDENIFMRFLPAEYRCYECITTENNPTLKEYLDSSNSSELLEEFKRYLALK
                     DKVIASGLNLNQIKKNKPAQSKFFQIQSLLYETYPNEENLHLTLPFQEIEKKIEQILK
                     TED"
     gene            complement(41933..42187)
                     /locus_tag="PLF66_03130"
     CDS             complement(41933..42187)
                     /locus_tag="PLF66_03130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63062.1"
                     /translation="MKQNLPIFILLFSLYCSSSPDTNTQVEPEKASATSNEKSMLEIL
                     KEKADSDEGKKVIETVKEKAQDPEMQEKIKSLFKKDDKKK"
     gene            complement(42197..42529)
                     /locus_tag="PLF66_03135"
     CDS             complement(42197..42529)
                     /locus_tag="PLF66_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001983891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63063.1"
                     /translation="MAGENSHFSKFHDLSYLDNLALFYLCNETPPSTLALAFLHGDPK
                     VVGSMLGVLDGKRREYVHSLMAQQKNVEVEKKESAVSGLLIIAENLISRNLIEKRGNF
                     YYGTERSK"
     gene            complement(42529..44022)
                     /locus_tag="PLF66_03140"
     CDS             complement(42529..44022)
                     /locus_tag="PLF66_03140"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002972937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HMZ63064.1"
                     /translation="MSQTLQSYRNFWKEYTRLGDIISLLHWDSEVMMPDDAREERAEQ
                     IAQLSSLSHKMFTGDEAKQHLENLEALIASNPPEKTILKREMEVFKKDRDRAVTLPIE
                     LVERFSKLTNLAHGIWADAKKNKDFKAFEPTLTEITELSIEMAECYGYTTERYDALLE
                     GYETGARAKDLEELFLHLKNSLIPIVNEGKSYSSPFKKPIPVELQKSFNEKLPALLGL
                     PASASRLDISVHPFSTSLGSKDKRITTRYHVDDPLSSIFGVLHETGHALYEYGVGQMA
                     NYPSPLTSAVSLGVHESQSRLWENQVGRSRAFWEYYYPILLKEFNIYHYDLPFNDFYN
                     YVNSVNKSKIRVEADQVTYNLHIILRFEIERDLISRKIKVSDLPEVWNTKMKESFDLK
                     IENDGEGVLQDVHWSGGAFGYFPTYTLGNIYSAQLFHHFLATHESFWDDLLKRGDVSS
                     LNKWLSKKVYSHGRILDPKDLIKEVTGELPKSDYLVKYLKSKVQEQE"
     gene            44113..44183
                     /locus_tag="PLF66_03145"
     tRNA            44113..44183
                     /locus_tag="PLF66_03145"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:44145..44147,aa:Gly,seq:tcc)
     gene            complement(44315..44632)
                     /locus_tag="PLF66_03150"
     CDS             complement(44315..44632)
                     /locus_tag="PLF66_03150"
                     /inference="COORDINATES: protein motif:HMM:NF016876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HMZ63065.1"
                     /translation="MENIYEIIISLPAQNDIKEIIKYYEEIRTGLGDEFLLSFEETIE
                     FLSNHPNIYPQIYKEFRRALMNTFQYALFYKVSLEEKEVEIIGVFHTSRNPEIWKTRI
                     PME"
     gene            complement(44616..44852)
                     /locus_tag="PLF66_03155"
     CDS             complement(44616..44852)
                     /locus_tag="PLF66_03155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63066.1"
                     /translation="MEPVLALEKIKKLPPFLQDHLEEYIDFLYIRYVATDSNFELSEK
                     GKQILDARWEEHLKNKNKAKTWIEFKKELNGKHI"
     gene            46011..46595
                     /locus_tag="PLF66_03160"
     CDS             46011..46595
                     /locus_tag="PLF66_03160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63067.1"
                     /translation="MQKAKINRFQWIAVFFLSFFFGCEHFLNFVLKDEELVQVPELVG
                     RWSAYEKGKETAVLKIEDFGKNHEYLLTVEKENKGALLRITQIKNIYVGEYHDFDEKN
                     QVKPVGYFFILKKVNHDFQIFIGSDITKKMVDQYKLVAKENQILGKKYITYSLSGTDL
                     QNRYALELLLSDPDFFHLKNSYNMILKRNSIQRL"
     gene            complement(46799..47563)
                     /locus_tag="PLF66_03165"
     CDS             complement(46799..47563)
                     /locus_tag="PLF66_03165"
                     /inference="COORDINATES: protein motif:HMM:NF017050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HMZ63068.1"
                     /translation="MLGKKWCGNGDLEGSVLNTPHNVIPTKGPVITAAIQYKFKPYPD
                     GYTHNAVIQDAGFPIGLGWYVSGKIPSVHFILRAVKVGIRYAKEFFFKLIGSPLQKGQ
                     VNIGDKLIDLLDSEKFVRSAFLLLGMGRDRNNGEIKLREDGEPIIKWEMKESQLHYDR
                     VRSAMKNVAEKMEGDFLDNPLTYLHKIIAVHPIGGCVMGESAETGVVDTKGEVFGHKG
                     LFVVDGSIIPTSLGPNPSLTIAANAERIAEQFPLKK"
     gene            complement(47512..48459)
                     /locus_tag="PLF66_03170"
     CDS             complement(47512..48459)
                     /locus_tag="PLF66_03170"
                     /inference="COORDINATES: protein motif:HMM:NF012935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HMZ63069.1"
                     /translation="MASSFDYDVIVIGSGFGGSVMSCRLSEKGYKVCLLERGKQYGMW
                     EFPRRINEIKEKLIWDPKDGRYGFMEVRDYPESDLMSVTASGLGGGSLIYANVLMRMP
                     EENFTGWPGGINRSTLEPFYQKVIDTMEASPYPIDHPYYNRTPKTHLFKKVGESLDTP
                     SDALEKPKVIFPDLAIRFKGEFAGHQTENSHGAIQSSCTKCGECDIGCNIHAKNTLDL
                     NYIFRAKKQKVAAEVKTHALVESISPLADDTGYEVIYSDPENSEKKTKIIAKQVVLSA
                     GSLGSTAMLLRMQKEGKLKNLSQCLGRSGVETEIWKDQF"
     gene            48718..49419
                     /locus_tag="PLF66_03175"
     CDS             48718..49419
                     /locus_tag="PLF66_03175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63070.1"
                     /translation="MEFFLENKYPKTWESLTLPPLYLNQVSEFGSINIFNDKNECIAS
                     GWFKFIISPKIIVLENLGEERKAFLENTRILDFIYQISSKNKFSVSKSIFLNNGKTLQ
                     LEFLEEEHKNYTLLTLTKPNPDLCKFTILNSYSFPGFWTIDTDGFWGKSFSVSLVVSS
                     AGYEIILENDLHMWKQWGEILPFYEGSTEWIITLLTSLNYKFIQKDPCQFQLESLSQF
                     LQLADLFTERDNTKK"
     gene            complement(49440..50318)
                     /locus_tag="PLF66_03180"
     CDS             complement(49440..50318)
                     /locus_tag="PLF66_03180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63071.1"
                     /translation="MPIQNEQLIGRFKKLLRPNDFLKEESVILKPYLKFLEKSYFEKS
                     LSSIEIISLFVILYQYFRVKDYPFQKNPINRSRIQINKEFPLEFTFIPSLDLETNYIS
                     EFLRLYSFRYLPNSIRDVLFDAFYNDRFVLVNYLPDSKEMLELQSCGKRYITIDFENA
                     FNGSLIEEKRDAFEFVLHDLEHAYCFFKKENDYERQIAFFSSVNKNYHLLEPILKESE
                     QFRKDFDYCVSDMNSHPDHLISYFKAILIKYFLIKNKKSEKDFLSSSELIPMNEILNP
                     IESDLFSFEHNGSRES"
     gene            complement(50378..50971)
                     /locus_tag="PLF66_03185"
     CDS             complement(50378..50971)
                     /locus_tag="PLF66_03185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63072.1"
                     /translation="MLRSVESNTYLGENKMLIQIVLILFFISSCNSNLYEYLNSKGSI
                     HTITNIPSKYCKDCRMEATIVTDDPYPYYHNPNIHAQINLTCNDLSRIKNMDADLLEN
                     KDILKPEKLEYFLEYKSIWKDLGNKNPSSHLKDFIEDCNDTNPIRLSIIKRYKRESLP
                     KKLEIILKAYFMDKDYEHRELLELRLTKDNRAPIRIH"
     gene            complement(51065..51508)
                     /locus_tag="PLF66_03190"
     CDS             complement(51065..51508)
                     /locus_tag="PLF66_03190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63073.1"
                     /translation="MKVKMFILFCFFLTILMSPVSAKISNRKKDSKNQGTKSGLDCTP
                     FYKGKFIEPGELGEKNIVVRDGTFSYFFPEGKSEYKMKSKIIVLENCMYKLVKLEYKD
                     PNLTEDDLKRVLEFQAIEQIYKIEKNKAFYRGVNCNCEGHYEKIE"
     gene            51937..54498
                     /locus_tag="PLF66_03195"
     CDS             51937..54498
                     /locus_tag="PLF66_03195"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HMZ63074.1"
                     /translation="MKYLVIILLLCFNVLNGQETIDNPATKVENKKQSEEEKFFSLEE
                     NIWVSVASKKPKSLRETHGIVNVYTSKQIEDYGFLNLSDTLNTIPGMRSGYALTQMPN
                     NLRSRGIANATLILVDGLPMIQKNEAYGLNNIPITIGAVDKLEVIRGPGSVLWGANAF
                     MGIVNIVTKDADEFIQDGKKSGYKVTMRTGNWNTNNFYFNSGAKLSENLNIYVGGSYE
                     STLGYVQKTNSTFTPNGFSVFQQPGNDILAQSNQDAINSGNTTPADLKIPWDRGTEGT
                     KNRRNLDSLAEFMVKANWKPYEGGNLKISLITNRNKFYFPISGALNQSDRNNNGYYEY
                     PYNIYSIKYSHTFSDTLNTTLTIGKYELTEWEHFLRAGAYYSSPSFSNQNSLSGSSSR
                     RNLDPDVTSQGNYLEKLSNLTIDWNVSYTGIKNNTILIGATYLEQGIPKLYFSRSTSL
                     NNSNQITQDRLPEWYDQGPVKSKIQSLYVQDSIRIHEKLNLQVGARYDKHYLSSSFIN
                     PQSSLIWNITSRLTGRTLYSRGFREPSYNNLFSYNGTVGSITYAGIDPDPKDKDPRAD
                     SPKKLNPEKSESYESQISYYLLSDWKLNINLARSNIRDLITPYYSNSQWTWYFNNGRQ
                     QIDSLEGEISYNLSKKLLFMEKGSVFFYNVTYNKITNFVTETESRDYTASIVNSNNPP
                     SEWAKNDFKLYTQDQRLIVKTDPRYWMNIGGVIRFLENYAFNFTAFYSAERYFGGYKG
                     LNSGTRFYPETDKDYLNIIAACGSNQTSSCPAIVNVNTRKNGVRIGSYYTLNIGLSYD
                     KIMENGSKIRISLQINNLFDRIYNAPYTNFSSTGAQILPNPQPGRQIYLTLTGYF"
     gene            complement(54538..56997)
                     /locus_tag="PLF66_03200"
     CDS             complement(54538..56997)
                     /locus_tag="PLF66_03200"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HMZ63075.1"
                     /translation="MQILVFIFSLLFLLFELHSQESSKKEESQQPKNSESSIQVKGER
                     SFSGITHMKEIEGTSILSGKKNEVLDLTKLNANLTTNNNRQVYGKIPGVSVWENDGSG
                     IQTAISTRGLSPNRSWEFNVRQNGYDIASDVFGYPEAYYTPPLEAIEKIELIRGAGAL
                     QYGPQFGGLLNYRMKKADPRRPFAVEFRQTGGSYNLFNSYNSVGGTTGKFSYFVFFHH
                     RSSDGWRKDSEYKTQTGFVNLSFQATEKLKFGIEFTKSLFTSQQAGGLQDGQMKYDPL
                     MTSATTEVNPRQSNRSRNWLSAPWNLPVFTVDFEQDEKTKASFKIFGLDGEKNSVGNV
                     AAINVPDTSRLTTGRTTGFEMEDAFSPRRVDRDWYRNYGSEFRFLKSYTLFGVNQSSS
                     FGLRYFIGNTLRVRNTNGTRGNDFTLSETNRIGNFVSRNSELNFRTMNYAAFYEHLLQ
                     ITSALSITPGVRYEWIQSEVSGHLSTDNLSVSSTPNSIDSRTTPYVMANSQRIRNKVL
                     LAGIGFQYKITKETNIYANYTQGFRPVLYQELFTPGNSVDYFDPNLKNQNGYNADAGY
                     RGAVSNFLTFDVGAFQLRYNNRVGTISANNRTDLIAALQNPRPNFRTNTGDSLSRGIE
                     AYLEYDPITHFFEKTSWGSISGFISYAQVKAEYIRSTNPIVIQSADFGRILLPDNGNL
                     KDLGIVGNRVENAPDRITRIGLTYTKKGLFSVTIQNSQVASVYTDANNTEHPLQANLN
                     TTGSALTSLTPNSQIGKLSSYKVSDLSFTWNITEILSLRGGVNNIENTIYATRRASGY
                     PGPGILPADGRTAYLGLGAIF"
     gene            57407..60148
                     /locus_tag="PLF66_03205"
     CDS             57407..60148
                     /locus_tag="PLF66_03205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63076.1"
                     /translation="MFKLLFYFLFLFILSCKNDLNQQNTDLGFLSFLIRLSMGAVNTS
                     NPIINPENDFYANDSLAPVLIARDRELGVKFGTEISSCDLEFNSTKITPIVRYASDTQ
                     TNYFSPQPGSYWPIDVVNPFTLKFSNCISKTGSTFDTSAISMKLYVAEKVIYIDGIGG
                     VDTNSGTNTLPVKTIPAGISLASASCSDRCAIALKGGEYILNSGIIVPTNLSIFGGFD
                     PIDWRKRRADKTALPPYDTIITDNSSGITSTGSDPYSTLKFLNYSGLQSKTVIDGIIV
                     NGPTTANSNSYISPIGSVALTSGSGFIIRNTISNDRSTSALSITSAGFSSITSSGSIL
                     IQNSTLQASPIVAATSTRYGIVYASSVTGSSFTLETSIVNGGVSSVGSSGFFATGSPS
                     GNILLSKNTIVSPDCSTICATVGVTMDFTTASGAIISDNTIQVTNGNNSRGISSSMGS
                     GIQIKNNMIQVTGINSGSVFGISFGGTSSNQIVDGNTITTSTAGAGGVNKGIDINGSV
                     SSINVLNNTINTGICPAASCARGIHKQNASNSTIVGNNITIGNCSNACDARVIDSAGG
                     GSNTIQNNILSYGAVTSGNPATGIELNSGNFTIDGNTINGGTCTGSVNCTGINISAAT
                     NITITNNTITAGSTTGTGNQIGVLMGAAATSFTFTGNTISSGVNSGSPANRIALDLVN
                     WPNNSTIHRNTFTNLSGSGNAIGVRFSTLANSLRFCSNLILAGNTTLGESVGINAQRL
                     NGGAKFLGNTIVLGTASVSASGMKFVTNSSYDNMEILYNIVQGNVAQTASTSCFVEQG
                     AITYITHQNNSVSNCSTYYNENGIVRSLFCSGNFGNGGCGAPPQTMQFPTATGNTISI
                     PVFVNPASNDYRLNTSSTPVDILTGMNNTATYTSLCGDILDRDGLARGLNTSIGAYR"
     gene            60295..61167
                     /locus_tag="PLF66_03210"
     CDS             60295..61167
                     /locus_tag="PLF66_03210"
                     /inference="COORDINATES: protein motif:HMM:NF021726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63077.1"
                     /translation="MFTSEIKNITGIHTEILTYRSTSNIQILILPGNPGIGSLYIDLG
                     KILYEKFNGQLNLYLISYAGFTKKKSEKKYTLKEELDHKINLIQELKKEWHPDSKVLI
                     VGHSIGAWLAKEIYKELGNTMNISLYMLFPFLAKSDNPMQVNFSKFLANPNHTGMSIN
                     AYKLITKLPASIFLSLAKLLYSHASENTFQLITDYFLLENHIIENIFYLANNEFETLT
                     EDFDLEFFQAYPKNINLYYCPDDMWAPLSQIETIKQNTKEVNAEILNTHTHDFCVSLY
                     QCEIIAEKIRTSLI"
     gene            61428..67007
                     /locus_tag="PLF66_03215"
     CDS             61428..67007
                     /locus_tag="PLF66_03215"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HMZ63078.1"
                     /translation="MKQSPLLLIGIVVSADGLSGLEDFFKALRDGSDCSFIIIQQQTD
                     SIENNLHKLLVSCTNLPIYKIEDGLHLEANSIYIAPPDQLVSLTEKRFHLEASPASSF
                     APINFFFESIVDWRERAVGVILSLDFEDGAEGIQAISQVGGLTLAVTPHTSQFATIQE
                     IASNIDFCDYKSTPSKMPELILDYAKEFSNKDSLDISETLKTNIFTLIKNTHGIDFNQ
                     YKVGTMNRRLKRRIELANLDSPDSYYAFLQKESSEIDLLVNDLLIGMTDFFRDPEVFN
                     YLETEILPSLFRNAKEDGIRIWVTACAMGQEAYTYAILLYQIAQKINYRGKITIFATD
                     VNKPSLAIASLGIYEPTQLSNLSNEQKENYFFYDINTDLYRIKPEIRQLITFAPHDLL
                     LDPPFTRMDFVSCRNVLIYLKPDAHEHILSLLNFALKMNGFLCLGGSEGLGRLSSTFA
                     SEHSNYRVYRKTKESRFSPEFRNTRSFSIPSIYPYDWNQSSNQTTTIDRDLLQDYDLL
                     LNRFIPPSVILNDQGQVLQYIGNVSRFLKLPSGRTENNILEMVDGDIYIAVSLLLSRA
                     RNKEPNLKAILVKLPDGDRVEVRVEVLIHKNNPSIHYHLFFSTEKFPWNESTVLPLKT
                     NPELQRRLFELETELQVGIVKERALVEQAQITNEELLATNEELQSANEELRSVNEELH
                     TVNSEFENQNFALNQLNLDHEQLLKSLQVGVLFLDESQRIRKFNSAAAEIFQLLPQDI
                     GRPLAHFSFQLEGSGNVPMDAIDAMKSGIPVEREVQSRDGKWWLKRAAPYFHSSGQIR
                     GTVVSYTDITNIKNTEEALRRSEAKFQAAIKHSSLIFAQTDKDLRYTWIYNPNPDFNS
                     ESMIGKSDIDISDNVGTRQLLQLKKEVLETGKGIQREIRFPFLNGYQTYTIIAEPLLD
                     TSGQITGVATCSLDITDRKQSEDALLESEAKYRNLVNNLNDGVVAHSADTSIQVCNEA
                     ACTILGLTMDQMLGKVAIDPTWKFVSEQGTPMDLSEYPVNQVLAKRKPIQNFILGVYR
                     PINSDFAWLLCNGFPVFKNNGEIDQVIINFIDITERKQAEDALRESENRFRVLFENSP
                     IGMAMIDSNTGQFITSNSHYYEMLGYTSEEFQNLSFKDLTHMEDLQMDLDNMERLKRG
                     EIQEFTMEKRYYHKNGSLIWVTLTVVPLWRMGDTPKYHIATVNNITEKKAREGLLQFI
                     ARRGWHSTKENFLDSIAQYLGEILNIDYVLIDRLKDEMTAETVALYAKGRIVPNIQYS
                     LLGTPCENVIGKEFCHYRSEIQRLFPKDSLLKDMKAESYLGVPLWDSDGNPIGLIAVL
                     DSKPMNDFQLAQTLLELVAVRVSGEIQRMEYLDVLKKSEERQRLVADTTLDAYYDWDM
                     ITNQVWRNQGYKNLYGEPTGNNLIWWKTHIHSDDYERLFKSMANAFSEQKSTWSSEYR
                     FLRNSGDFAYVLDRGRIFYNEKGKPIRFIGAIIDLTERLQFEDELKKAKWSAEKANNA
                     KSEFLANISHELRTPLNGILGFAQTLKLIAEHVSPVILDGLRIIENSGNHLLRLINDI
                     LDLSKIEASKIELSLNPVMITDLLEKIIDIVKQNAIEKGLDLKLDIATDLPTIVITDE
                     KYLIQILLNLLGNAIKFTEKGEITLKVSRNGEKILFALIDTGIGIPQDKLEEVFNPFQ
                     QLNNSSKFEGTGLGLSISQRLVQSMGGKIQVESKPGSGTKFWFEFDLPNEKANLEKFE
                     LSEDLINQYDGTNKRILIIDDDEINRMVMRAMLLPLGFEVIEAEDGKIGIEKVQVFKP
                     DLIFMDYRMPGQNGLETTILIRQMILEIQPKIIMCSATALKELREKSLEMGCNDCITK
                     PIKLKDVLNCLQSNLNQ"
     gene            complement(67249..68676)
                     /locus_tag="PLF66_03220"
     CDS             complement(67249..68676)
                     /locus_tag="PLF66_03220"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025033.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HMZ63079.1"
                     /translation="MKKKDRRNTIRIRLHDVEKSYILLLINGRELKGKIYDYSRFGLG
                     ICIPKSESAFIKKTQPIENCIIFAYGDKKELGTGTVVHLDSMNDEIFLGVFLEREFID
                     MDTLMDKQSLFLQEDEVKKIRMQFTIQEDLEPNFILFCSKFVYGLSLYKIALDELDDK
                     FAKEPNRLKEALFQSVLKGVGGEFYEFLTAKIEELKVLTKEFTKLQNEKYGFYLRKSI
                     WNYILESEFIKRTNLKPRGYAGDSAMMEMLYRNEYLGKSSFGKIFHKHPCDTKAADAV
                     RNRRRLINKYMSQRLSTSGKDDFKILSIACGPAWEMQDFLRESPYKHQAKVYLLDQDE
                     EALGEAKSGIDSIPTEKPFQVEFIKESVRTILKTGSPELKFGQYDFIYSMGLYDYLTD
                     SVAKVLTEKLYSMLCPGGILILGNYHVENETRKYMEYIMDWVLYYRDEEAMFELLEDI
                     PKGFTSEVGFDDSGTQMFLRVEKNR"
     gene            complement(68684..70042)
                     /locus_tag="PLF66_03225"
     CDS             complement(68684..70042)
                     /locus_tag="PLF66_03225"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HMZ63080.1"
                     /translation="MTSISNKPRAPEPFDFVDSFSDYLDFILHSWLKILTILGFTLVP
                     IFFILDIFIIPSEAKYLLPKFAAYRVTATAIVILQYVLIRKTKPSKFSLLHGYAFTFF
                     VSLAIILMTVDLGGFNSSYYAGLNLVIIAVNLLLPWRAIHSALNGFITVVFYVIFNAI
                     WGKDFYIYNLVNNLYFMLSTVVIAASINHVKYVLIQKEFESRKSLKEARDALWSEMEV
                     AKRIQTSLTPPDDKLGGYEISAIMLPATEVGGDYYDIIRTNKNPWVMIGDVSGHGVES
                     GLIMMMAQTSVRTIISKNAEEKPSQVLVELNKVIKENIALLKVERYMTITAISLSNEG
                     MTYAGSHQDIIVYRNKTKTIETFPTYGTWVGMLDDVEVFMQNHTLKLESGDLALLFTD
                     GITELDYQGKEMFGQERLKESLLKYNQLSGREIIQKIVEEALSTAKSQDDDISLVLLK
                     KV"
     gene            complement(70048..70410)
                     /locus_tag="PLF66_03230"
     CDS             complement(70048..70410)
                     /locus_tag="PLF66_03230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63081.1"
                     /translation="MSEVKTYEENRLKIEVRYGEADILINWFGESKDREPTKFLTPIF
                     DAIYAKNQTKPLILNFTQFEYMNSSTVTPIVKQLERAKKENRKVTIHYKKGLKWQELS
                     FAALRVFNIPGLIEVVGI"
     gene            complement(70426..70923)
                     /locus_tag="PLF66_03235"
     CDS             complement(70426..70923)
                     /locus_tag="PLF66_03235"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HMZ63082.1"
                     /translation="MSNDSSNLLILQLKADWSELDRVSDSIQTFFANSDLTKDDLDAV
                     KMVTTELVENGIKYGYYPNPETTLELILRKKKKRIVMEVKNQVKDSELVHLRRLDAKI
                     QWIRGYQNPFEAYVERLKEISNKNLAEGESGLGLVRIAYEGQSILDFYVNEDNLLAVS
                     AVYQY"
     gene            71116..72666
                     /locus_tag="PLF66_03240"
     CDS             71116..72666
                     /locus_tag="PLF66_03240"
                     /inference="COORDINATES: protein motif:HMM:NF016158.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cryptochrome/photolyase family protein"
                     /protein_id="HMZ63083.1"
                     /translation="MTSFYKLIKQFRPKQDDLKNLNWNWILYDQLSNKHKLIQNTVQD
                     GLIFIESKAKPECRNYHKQKLVYVISSMRNFAIEKGKEGFKILYFYSEKDYSLALEEL
                     KKKYTLNQIKCLEPSEYEVYRSIQKLTFLKILDNDLYISNLEEFQSIFKNQKNFKLET
                     FYRYMRKKHSILVKNGLPEGGEWNYDRLNREPFKKRDKIPKRIDFSLNAVTKEVIELV
                     NSIYKNHFGNTNLFNYATTRQEALVALEFFCKHFLKEFGKYEDAMSIQEPNLFHSLLS
                     PYLNNSLLSPMECIQKGIEYYEKKEVPLNSIEGFIRQILGWREYMRLVFISNRENYLQ
                     ENFFKHTSKLPSFYWEGNSGMECLDQTIKQVIHTAYSHHITRLMVLSNFANLLGVNPK
                     EINDWFWSMYIDAYEWVVVPNVMGMGTFADGGKISTKPYIASANYIQKMGTEFCKNCK
                     YKPKEIFTEDACPFNSLYWDFIHRNKEYYSKQFRDYSYLNLSKMEKSKLNKILEKAKE
                     YKVKFLDK"
BASE COUNT        24435 a        12744 c        12213 g        23470 t
ORIGIN      
        1 gattcgctgc tgttaaaaaa ggggatgtat acggttttat caaaaaagag gatatagcaa
       61 aggaagtaga gattaaatga atttggaaaa atatcatcca acaaaggttt ttagatattg
      121 tccaagatgt ggaaaagaat cctttcatcc aagttctgaa agatcaaacg aatgcagtga
      181 atgtggattt gttctctact ttaattcagc ttccgctgtt tgcgcactca ttttcaatga
      241 ggaaggaaaa attctactca ctcgtagaga gagagaccca ggcaaaggta tgcttgatct
      301 accgggtgga tttgttgatt taaatgagtc agcagaaaac gctctgagta gagaactaaa
      361 agaagaatta aatctggaaa tttctgatcc caaatttata gcaagctttc ctaatgaata
      421 tttatttaaa gatttattgt attttacatg tgatctggca tttgtctgca aagcaaattt
      481 tacaggtaat atgacaccta acgatgatgt gagcgagttt ttatttgtag acttaaatca
      541 actggacttg aacttaatag cctttgaatc aattcgtaaa gttctaagat tctatttaaa
      601 tagtaactga cattacgcac aagatagaaa tttatatgga aaataataaa gattattata
      661 ttatgtttct caaatggatc aataaataag tgggtttggg cggctagccc aagtgatgca
      721 ctgagcccca gtcgaagtgt tgccgacagg gctccctatc tctcatctta cgcgctgatg
      781 cgtaaggcga gatagtaaaa gataatttaa gaagtttaat taaatgaatt aaagttatct
      841 ggtattgcta aaatcataaa ctagtttcat catgagtcca gggtttcttg ttacgaaacg
      901 aattacatca tcatgcgaaa ctttatagag agatacagag tcttcattaa tatatgtata
      961 ttcagatgga tgcccccttc tgattctaat gagagaacct acataatcac cacgtttaag
     1021 aatgcctttt agctttcctt tttgttcaac ctttacttct cctttgcgga taatgtaaat
     1081 ttgctccatg atgtgacctt cttgcataag gatagatggt tggtcaattt caatgggagt
     1141 catcatagat tctagaaggg ttttttgtgt agcagttaac gcttgaaatg aactactcga
     1201 taaaacattc catgactctg catcgcgcat tctagcaact tttgcaagag tcttttcgaa
     1261 ttctgttcca ctgataaaac ttaaaaactt atctttggaa atagtataca ccactacatc
     1321 agtttctgca atcacagctg ccgttctttt ttgatccgta atgagagcca cttcaccaaa
     1381 atagtcatat gttccataga cctttttgga aaccaatccc tctgacacaa cagatacatt
     1441 tccaaaataa atgatataaa atttatcagc actgtcgcct ttgtttatta taatttcacc
     1501 ttttttaaag gttacctctt ccactatgtt aataaattcc attgctttac ttatggagag
     1561 tccctcaaat aaatctaaat tctttaatac acctaaaatc tgactcgcct tttcgaaacg
     1621 gggtggagtc atcgggaaat acaatgtatt ttcaattcca aattttgcta gagtcaaact
     1681 tgtctctttt ggaaaatctt ttgcagcaat atgataaaca accgtcttct tttttacttc
     1741 ctcaggtaaa gagtctagat atttaatcgg tgtatgcaaa ggcggaacac cagattcatg
     1801 aaaaataaca tttgaatccc atggaaaatt tcgcaattcc tcataacgct cttggctaat
     1861 gattccttct cgtaaaaact tttcatgaat ttctggatca ttattatgat cggaagaata
     1921 aacaaaagac tgatcttgat actccatttt gaaaccgata gttggaatgg aatggagcgt
     1981 atagaacatt tgaaattttc caccatagat aaattctggc ttattaattt taattggttg
     2041 aaaagtaaat agactcttta gatattcttg ggagacattc gtgaatgtag aatacttata
     2101 caaaaaactt ttcatgattg tttcggttgt atagatcgta atcttacctt cttccaaaat
     2161 tttctgaaag gttcctgcat catgatctgc atgacaatgc gttagtatga tagaatcaat
     2221 aaactttgga ttcacatttg aatctaataa ccactcggta gaatttactg gtggatcaat
     2281 catgattcca gaatggttca accaaataat aaaaccagat gtattttcct ctggatcaaa
     2341 tccatgactt ggtccaagac aagtcactcc aaaaagagga ggaataaatg gctctcttaa
     2401 acgctcccct attgcataaa aacctttgta ttccattttg cctggaacgt cagttacatg
     2461 attcgctcca attgagacag aaaagtctcc attttctttc tttcggatag ttgtcccatg
     2521 tattgtaaac tgattgtctt taaatatccc aaacccaacc atatcctgaa acttaaaagt
     2581 gctactaaaa aaattatgct ctgattttat atctggaata tacgtattac catttaacaa
     2641 ctcgtaatca caagtaaggt ctaaattgcg aggaccaaag atagattctc ttaaaacttc
     2701 tttaaatcta cgatattgtt tctcgctgca aataatgaag gttttttttc ttctgaaaaa
     2761 gaaattgtaa tagataggaa attctaattc agcaacgcta attcctttcg tccaactgaa
     2821 aaattcgtta ggtaaaacaa atacctgagg aacacctccc tctaaaaaca tagtgtcctt
     2881 aattgtctca ggaggtgacc caaattgtat ggatgaaata tctgtcttaa ctaaataacc
     2941 cccacgtggt agggaaatta gacagggatt tttttcatca cgaccactca tttttaactc
     3001 ttacagacta ctcgaaaggc tacaatacaa agagtcaact tttttatgat aaaaaattta
     3061 tttcgatgaa aagcgatttt gttttaatta aaatagaatt ggatacttat cgattcaatg
     3121 ttctagttaa aaaatttcta aataatcctt ccactgtctc aatagaacta tttagtctat
     3181 gatcaccttg aattaaatga agcacagatt tactctctcg cgcatagcgg atagataatt
     3241 cataggggat tacttcatcc tcccacccat gcacaatctc taaattttct attattggat
     3301 acttttgctc cttgaatcca taaatataaa cagcaggcgc taataaaaaa acacccagcg
     3361 gcttaaactg atttgatgca accaaagaaa catatgcccc catgctagac cctactaata
     3421 taaattcaga atctactttt ctatcactta gaattttgat aagtctatct gcacgaacct
     3481 ccgggtccat cgtatcagta taatcaatac tttttacctc taacccgaac gcctctgcta
     3541 ttttagaaag tctctgaatc ttgctaccgt ttggtccact ctccttacca tgggaaaaat
     3601 aaatagttac gtttttagaa ttgaccaata tttaaaatcc actattggat agagaaacac
     3661 aaatcgaatt tacaaccgaa accaaatctg gatgagatgc ttcaaattta ctgacacttt
     3721 ccgataagcc cttaattgag agatctatta aatccttatt tttatttgat ttaattactt
     3781 cgtgcgtaga tgcttttgta aaattagcaa ctgtttcagc atcctcctta ttcgttgcag
     3841 aaagctctac cacttccttt tttaaggaga ctacaagccc taaaagctcc tccttttttg
     3901 ccacatctaa acttttagaa tctcgaatat tttcttcaat ttttgtaatt gtatcttgaa
     3961 tcattgcaaa ccctctcgaa tccaagcttt atatccctta ggaaaaggtc aagaatcttg
     4021 aatccatcca aattgtaaaa atagctccgc cccttggttt tgtgcggttt atctgtgtta
     4081 gccgttgtaa gatgggaaca tgcgtctatt agccataacg actctttcga tattgaaaaa
     4141 taactgtgta gtttagccta ttgttcataa gaaagatctt tgcgatcaag tcgaaatttg
     4201 agatctcagg aattccgact gccaccaagt ggtgcgagga ggcttttgca aaatcgacgg
     4261 aatctgctat aagtaggtat actaaaaaag ccgagatgga cataccaaaa cttacacgat
     4321 aaagcttcag ttcagcccaa tcttctggtc taggacgaaa ggctatctta tgtaaatttt
     4381 gccccttttc ttggtatttg gttgtgagat ttgcataacc tggaacaaga ccatttgcaa
     4441 tataaagttg atactttgaa agaagatagg cgatatactg tcgaactcct ccgtgagcaa
     4501 ggcatttctc ccaaaagagt tgatggtatt gatcgggaat cagaatagtg gagctaaatt
     4561 ctgaatagat ataatctttt cttggaaatg caaatttctg atttgaaaaa agctttggac
     4621 tttccttcag gaaactggat tgatgattca taaaatacct ctttataagc ttatccatac
     4681 ccgcaccact gataaagaag atgggcaccg atgagtctta actataggtg agttttgttc
     4741 tgttccatga tccattgctc tcatcacatt tagtttttaa agattcaaag gttcgtcctt
     4801 tcaaattatt tcaccaaagc cattctgtcc gaatgggaat tcatgtttta tatcgttcag
     4861 tgattggtgg agatccatta aaggaagtgg gtaaggatat ttaatataag aggggaaaaa
     4921 aattaacttt gtgagcgcga aaaaagaaaa aaagaaacaa atttaggtaa aaaatataaa
     4981 agtaatcctt ggagtcttcc tctctagatt gaaattggag cttgggtaaa atagatacat
     5041 tcaataattg tattacagaa caaaatttga gccctaatga actcaaaaat tcagatttac
     5101 ctatgataga agagtgaaaa cttggaatta tgaaaacaat gtttgaaaaa atttggaatg
     5161 accatttagt aaccgagaat aacggttccg ctattctcta cattgacagg cacttgattc
     5221 acgaagtaac aagcccacag gcttttgaat cgcttcgttt atccaatcgt aaagttcgcc
     5281 gaccggaagc aacatttgct accatggatc acaatgtgtc cacccgtagc cgagacatgg
     5341 actttgcaga tccaatctct gcaatccaaa tgaaaacctt aattaaaaac tgtaaggaaa
     5401 ataatattac cctctatgat ctgacaaatc cggatcaagg tattatacac gtaattgcac
     5461 ctgaactagg actcacacat ccaggaatga caatcgtttg tggagattcc catacatcta
     5521 cacatggagc ctttggtgca cttgcatttg ggataggaac gagtgaggta gaacacgtat
     5581 tagccactca aacattagtg caacaaagac caaaaacaat ggaaatcaga gtggaaggga
     5641 aattgcttcc tcatgtaaca ccaaaagata tagttcttgc tatcattgga aaaattggaa
     5701 cggatggagc aacaggttat gttattgaat acactggtga ggctattcgt agtttgtcga
     5761 tggaaggtag aatgactatc tgtaatatgt ccattgaagc cggagcaaga gcaggattta
     5821 ttgcgcctga tgagactaca ttcaattata taaaaggcaa agatcatgct ccaaaagaaa
     5881 acttcgattt atttgtaaaa aaatggaagt catacgcaac agatcctggt gcgacctttg
     5941 ataaaacagt tatattaaaa gcagaagaaa tttctccaat ggtaacatgg ggaacctcac
     6001 caggacaagt aatcggcgta acagaaacag ttccaaatcc agaatcggca accgatccaa
     6061 cggttaaaaa atctattgaa ggcgcgctcg cttatatggg actcaaagca ggccagaaga
     6121 taacagacat taccctcaac aaagtattta taggatcttg cacaaattct agaatcgaag
     6181 atttacgaat cgcagcaagc acagtaaaag ggaagaaagt ttctccaaaa gtcaatgcta
     6241 tcgtagttcc aggttctggt cgcgtaaaac gtcaggcgga aagtgaaggc ttagataaaa
     6301 tttttatcga agcaggattc gaatggagaa atccgggatg ttctatgtgt cttgccatga
     6361 acgatgatgt attgtcacca ggagatagat gtgcctctac ttctaatcgt aactttgaag
     6421 gtagacaggg taaaggtgga agaacacatc ttgtgagccc agcgatggca gcagcagcag
     6481 ctatcgaagg taaatttgta gatataagat attggagtta aaatgaaagc atttacaaca
     6541 ttaaacggac tagccgctat tttagataag gcgaatgtcg atacagacca gattattcca
     6601 aaacaatttt taagaaagat tgaacgatct ggatttggag ttcacctctt tcacgattgg
     6661 agatttttag atgatgcggg aacaaagcca aatcctgaat tcatattaaa ccaaccacgt
     6721 tatcaaggag ctagtatttt acttgctcgt gataattttg gttgtggttc ctctagagaa
     6781 catgcacctt gggcactcga agattatgga tttagatgca tcattgctcc atcttttgca
     6841 gatatttttt acaataactg ttttaaaaat ggtatgttgc ctatcgtttt accatctgaa
     6901 caagtagaag aactttttca atatgtagaa aagaatccaa atgcaaaact agaagtagat
     6961 ttaatgaatc aagttgtcaa ggcggctaat ggcaaatcct attccttcac agtagatgct
     7021 ttcagaaaag attgtctcta caaaggttta gatgatatcg gactcacact cgttcatgaa
     7081 aattctatta ctaattttga aaaagcaaga agaagtaaat accgtaaatg tttacgagag
     7141 aataagaaga agtgaaagat tgccaccgat aaacaccgag agcattgata aataatacga
     7201 tatttcttaa tttagatagt tttatttaat ttgatttttg tatcgttctt ttatcggtgg
     7261 tgaaaaatgt tttcataaaa aggagagaaa atgttcaaaa ttttattagc aattagttta
     7321 tttgcattcg caagcgttaa cgtgaatgca caggaagtga aagacctaat cgtaaaagat
     7381 gttaaggtcg gaaacggaaa agaagcaaaa gacggaaata cagttactgt gcattacgtt
     7441 ggaacgctta caaatggaaa aaagttcgat agttctaagg acagaggtca accatttagt
     7501 tttcaacttg gtcgcggcga agtgattaaa ggttgggaca gaggtgtaaa aggaatgaaa
     7561 gttggcggca cacgcaaatt aacaattcct gcacatttag gctatggtga cagaggtgct
     7621 ggatcagata ttcctcctgg agcaactctt ctattcgatg tagaactttt aaaagtagac
     7681 taatccaatt ctaatttagc ccttctctta attcaatcca ttttagtgaa gggcttttct
     7741 ctttctaatc atccctcttt tcaaatatga gaatacaaat tcgtctattt ctataattat
     7801 cttataaaaa acagaaaacg ttagttcata attcgatgtc caaagaaata tatacagaat
     7861 cagaattaaa cctctttaaa aaatcgcaaa aacttgctta caattcagta ttagaagcag
     7921 agagtaaatt aacagaaggc atcacagaaa aagaagccgc gcatttactc gatgaaattt
     7981 taaaagagaa aggcgttaaa aatttctttc atacatcctt tgcttggttc ggagatagat
     8041 cttgcttcag aggatttact aattattttg attttcaacc tacaaatcga aagttagaaa
     8101 aaggaatgtc tgtcatccta gataccggac caagcttaga tggaagagct tgcgatattg
     8161 gatattcttt ttcctttggt gaaaattctt tagttgacaa agcaatctta gacttagaag
     8221 tctttcgaag ttttatttta aaagaagtct tagctgagag atcgttaagc gatatatatc
     8281 tagaagtcga taaaataata aaagagatgg gatactttaa ttgtcataag aaatacccag
     8341 cagaagtatt aggtcataaa attggcaacc tccccttttt acagattcca cccattaact
     8401 tttttggatt tcattctcaa acatttctat atttacttgg tgaaactgct aatgctctcc
     8461 ttccaaattt tcttttgcaa ggcaataata cttatccttt ctggaatcat tcatcaaata
     8521 caagggcaga gtcaggactc tgggcggtag aacctcatat tggaaaagag gatgtcggcg
     8581 ttaaatggga agaaatttta gtagtaacag aatcgacagc ttattggtta gatgaggagt
     8641 taccacatgt tagtctatgg aagaaaaagt aaattttaat tttattttta tattaatcta
     8701 agaatctcct ttgaatgctg tcattatagg agaattttat gaataaaata tttttatttg
     8761 ccattttagc tctaacgcta acattcagtt catttgctca aaccgctgaa gaagatccaa
     8821 aaccatgtaa agaagacaga caaacacttt gcaaagatgt taaacctgga caaggtagaa
     8881 tcattaaatg tctaaaagaa aatgaggcaa aactttcgcc cgcttgtaaa acacaccttg
     8941 aagaaaaaac tgctgaagca aaagaaaacc acaataaatg taaagaagat agaaaaaaat
     9001 tctgcaaaga cgttaagccc ggagaaggta gaatcatcaa atgtcttaaa gacaatgaag
     9061 ctcaattatc agctgactgt aaagctgtaa tggcagaaaa aaaataatct cagcttaaac
     9121 attgtaaaga cttattcaat tatagctttt atgcaaagta gtgattgaat tggatataaa
     9181 aatccaaaaa gtaagtcttt acagaaacaa aatagattaa ttaactcacc ttacacaagg
     9241 tgaacccaag agctccaatg aggtttgccg tcgaaggcta ttgattttaa taattcatta
     9301 gcatttccta atattttcaa aatcatttct ttttcctcaa attatcaatt ttgcgtgatc
     9361 tcagttaatt aaaataatta cttccctttt ttatccttct ctatttgaag caatagcttt
     9421 tgaataatta aatttagaag actaatacta ttttcataac tctcacctgc ttctttcgcc
     9481 atacgaatac tattcatagt cactagatga gcatctttgt atttaccttg tttgtataaa
     9541 acctgtgcca atgtttcatg atttagaaaa ttaggttcta attgaattga ttttcgaatt
     9601 aatacctcag cttttgaaag tgtcttagga tcttttaact tttgcgatat acgccatgca
     9661 attgcatcca aatccttcca ttcattcata gaatattttt ctgcataagc aatcgcaaca
     9721 gttgcatagt ttttccaatc ctctgccttc gagtagtaag ccatctttgt taagtagatt
     9781 ttcttttcta cattgtcttt accgtgacta ggataaaatt tttctctatt tgctgttaga
     9841 tatttaaatg tttttgattt tatatctgtc acaaaatcat caataatctt ccaatttttc
     9901 tttgatatta aatcatcctg tgtatcaaag taatcttcta gtagatcacc atttacttca
     9961 ccaaatgctt tataagaagc aaagactaat tcataaagag tcttatcatc ttttgctcct
    10021 ttttcatatt ttcgttttaa ttcgtaaaaa gctttttcag gtataaaagt attgcccact
    10081 tcttttagaa aatcatctac ttccagtgga ccttggaatc tatgtataat cttacccgtt
    10141 gaatcaaaaa ataaataagt aggatatgca tgaacattgt atttttctgc aaaagaaaga
    10201 ccttcttcct tctccatatt tagtttaata cttacaaact tttccgaaaa ataatcccca
    10261 gcttttttag agttgtatac attttcatcc atccatttac aaggactaca ccaagttgta
    10321 aaggcatcga taaaaataaa cttcttctct tttgtagctt ttgcaacgac atccttccat
    10381 tctgaattta caaaggaaat tccaacttct ttcttggttt caacagcttg ttctgttttt
    10441 ttagctggtt catttacagg acttgcctgc ggcaaaattg aatttaccgc aagtaaaatt
    10501 attaagaaaa tgcttttgtt ttttttcatt tatgatcctt agtcttctgc taagactaca
    10561 attttgtctt ctgcattaaa tcgaattaga tcagattttt taggattaat caagactccg
    10621 taggctttct ctgaatcatt tgcatttgca tgaatacgat aaccgatagc tatctgattt
    10681 ttctctgcag ctgattccag aattgtatag aaattcattg gatctccagt cttaacataa
    10741 tctgtaactg gctttaaata aacttctgat ccctcagcct caaagagtat atcaaatact
    10801 ttctttaatt ctttcttctc tgagagttgt gaaagcatta aactaatgat tcgatcactc
    10861 acgatataat catctgcttt tgtaacttct gcaagtgcac gatttttatt atctaacatc
    10921 tcactcacaa tgcttagatt ctttccagtt ttattcgcaa tatctctaag atgcaataga
    10981 catatcaaag tttttgcatc cgcttcttgg atgtctattt tgttagtgta acatagaata
    11041 ataatatgac tgtaagtaga aacttctaaa gagtccaaaa catttctatc tgtaatattc
    11101 gcttgtttta cagtcacttt ttgttttttt aattctaatt ccttcacttc ttcttcagtt
    11161 tctgaaagct cggaaacgat cgttacttca gaaccattcc caacataatt ttctaattca
    11221 tttataatac gatatccctt ctcattccat ccaagaatca aagttctttc ttgctttaat
    11281 ccctctggtt ttggggattg aaataaatta gatttaatat cataatttga tttttctgca
    11341 agatcaatcg catcatcatc ttcagcaatt acaatgatac tgtctttctc agcaaacaca
    11401 gtatccattg gtggattaat taaaacttca ccactgcttc tcataattcc aatcacagca
    11461 gatgtttcat aggaaaataa tatttcttta aatgttttgc ctttaagagt agcctcttct
    11521 ttaaaataaa tctctactcc atcaaaatca agcagctctg tataaaccac actaagtcct
    11581 gattgtctac aagtttgggc agtcacgcga gcaattaaat caggagatag aacaaatgta
    11641 gcttcatctc ctccaactaa cgcaccagcg gctaaatttt cttgatgctt catttctgct
    11701 acaatgtgat atttaccttg ctttcgatgt gggttatttg taatcgctag aatcgtttta
    11761 ataacactta tatccgggtt ctcatcgtca ggcgacatta ctataattga tttagcatca
    11821 tctagactca caacttctaa gtcgttgaca tccataggac tgccagttct acaaataatt
    11881 tttgttgttt tacgatcagg aatttttgaa tttaattcat cttccatttc aactttatct
    11941 ttatctgcaa gaatcacaat acttgctttt ttcaaacttg catttgcaat aatgatttct
    12001 gaaataattg ggtaaatttt tccactccaa cctagaatca aagtatgatt tttctctaag
    12061 actctagatc gaccttttct tagttcttcc aacttagctt caagacctga agtaagaacc
    12121 ccaatcaaac tacttagaat aaaaatccct cctatcgtca caagtagcat taccattcta
    12181 aatgaccaac cctgatccgc gcctaacgct cctgcatcga gtgcatgcat taaactttgc
    12241 caaaatgctt caaagaaatt taatttctct tccgggttct gtgaaatttg accaaaagta
    12301 agaacaatac cagcaaggat gactatcagt agagaaccga atgctagcca tccaataata
    12361 gcaatcgttc ctttggaaag agtattttca aactcgtatt taattcgttc cttaattcca
    12421 acttttttat tcattgcttt atcccaaaat atgatttcaa tttttacttt tttattttcg
    12481 gcttattttg gcaaggcaag ttaagattta agccaaatat gttaatgatt tgatatttcg
    12541 aaaatgtgaa tcatagattc tttatgctta caccatttgt cctgatcttc atttcttttg
    12601 tcagaaacaa ccacaatctc atggtgacca agccatgaga ctccctcaat attacaatat
    12661 ataatctcgc cacctaaccc agcattaccc tctctatctc caaaagggaa ataatacaag
    12721 gtccctttct ctaatacttc ccatttttct gagtgtaatt cccctaccca tagcgcagaa
    12781 gacatttgag aaacaatggc aactcttcca ttttcaatgt ccatatccga ataatccaaa
    12841 aaagagattg aggatggtaa tttaatttct gccgttattt cccaatgatc tttttcttcc
    12901 ttcaacacta gaatccttcc attccctcga tgtttctctt tatcactatt gcaaaaattt
    12961 ccctcacata aagctaaaat ataattttct ccatttcgac taacatgact tatcccttcg
    13021 attcctttat ttttcttctc caactcaaat ttcacaattt tctcttcttg aaaatttaaa
    13081 tctgcatcca gtcgaacaag aaatggataa tatccattgg taatttcttt ggattctatc
    13141 ataacataaa actgaaaggg attctcatcc ttataagtaa tcgcttcata atttgatttc
    13201 tcaaaaatat ttccctttaa aaaattcatt tggttgttct ctaaggaagc ttcaatcaca
    13261 ccgactgctt tcatattatc aaaaattaca taaagttgat ttcctctagc atacacacca
    13321 ctcccctcaa aatgaaaatt tttttgatag cctttaaata atttataaaa cttctcctct
    13381 cgaattgcag gtgacaggac aatcttacta gtagtccaaa aacttttaga acttgtttca
    13441 ttcccaaaga gttcatgatt ttcttttttg cagaaagcaa ctaatagtag agtgaaaatt
    13501 aaaaagagga atcgaataaa attcataacg caaatctatt taaaatattt tgtatgtcta
    13561 tctaaaactt tgttgtataa aacaggaatc atttcactct attcgcaaat ggattttgac
    13621 tttttaaaac taatcttaca agctcctgaa aaacaagaca tggttcctat gttagaacta
    13681 gcttttcgaa ttggagttgc caccctactt ggatttttga tcagtggagt ttcctattta
    13741 acgtatacag gtaaaaatta cgatcgttct gttattcatt ctcaaattat agtgactatt
    13801 gtcttttctg ttatgatcaa tgtaatcgga caaaatcttg cttgggcagt tgggattttt
    13861 ggttcattga gttttattca atttagaaca acccttcgag atgcaaagga cacagctact
    13921 tttttttatt ccgttgtaac aggagttgct tgcggagcag gttatatcaa tctagcaatt
    13981 tttggcttct ttgttatgag cttagttcaa gtgatcttaa aatatctccc acctctcgaa
    14041 gttcattaca catatataaa atttaactgt aaaaatatag aagccaaaga aatgattaaa
    14101 agttttttaa aaagagaaag agtcaaatac gaattagccg caatatccgt aaaagcagaa
    14161 aatgttattt ttgctgtaag actcccaatg gaatcatcct atgaacttgc taaaaaagcc
    14221 aaacttaaac tcccggaatg ggtcgatagt tttagcctag atagagaatt ataattctac
    14281 agaagatcct atagttaaat aatgaatatt caaatttaac tgtttttatc tttcattata
    14341 attctgagta agtcaattat tctatttaaa atgagattgt aaaaggctaa ttccaattta
    14401 ccgctttttt aaaataagtt gcgaaaacta ccaatccaat tagcttagat attcttaagg
    14461 aaggaaataa catgaaaaaa actattttat atattggact ttctatctta tctctcaatc
    14521 tatttgtttt atttgcagat tctgcaaacg caatctcttt aggacaagta agtgctgtca
    14581 cagataagac tgtaaatcta tccgcaagtg gaactcccgc acccggagca ccgaaagcct
    14641 cttctatcaa gaaaggagat attttagtta ttctcaatga agacaacagt gttggagaag
    14701 taaaagtgac taagacgatg caaacaatgg gcggtaaaaa ggttctcata gaagcagaga
    14761 ttctctctaa atcaatgcca atcaaaacag gtatgggatt tagaataaaa taaatctttt
    14821 tttaaattct gggctctgta aatttctact taaaaattag atctaatttt taattcttga
    14881 aggggattat ttccagagcc aataactcct aaagacttgt tttttcaata tgcaagcaag
    14941 atgaaaaaac aacaaatgat gtctttttct actctcccaa aaaaactctg ataaaaatca
    15001 taagtatgta tttctaaaca caaaatattt tagttgtggc tgatcattgt tacttactta
    15061 aatagatttt gtaagtagaa cacgatcctt tttaagcagg taaaaaagca tgggaaaata
    15121 taaagattta tttgaagagt caattcagaa tccacaaaaa ttttggaaag agcaattaga
    15181 aattgtaaaa tggtttaaag aaccagaaac tattttatcc aaagataaag acggacttta
    15241 tagatggttt gaaggaggta aattaaacac atcttattta gcacttgatt atcatattga
    15301 aaatggtcga ggaaatgata tcgcacttat ttatgattcc cctgttacaa atcaaattaa
    15361 aaaatttact tacattcaac taagagagga agttgcaaaa tttgctggag tcttaaaaca
    15421 tttttcttta acaaagggag atacggctat tatttatatg cctatgattc cagaggctgt
    15481 cattgcaatg ctagcttgtg cgcgactagg tgttgttcac tctgttgtat ttggaggatt
    15541 tgctcctcat gaacttgcag ttcgaatcga cgactccaaa ccaaaattaa tgatcactgc
    15601 ctcctatgga gtagaaatcc ataaaatcat tccttacaaa ccgatgttag atgaagcaat
    15661 tcaccaagcc attcataaac cagaacatgt tgttttatat cagcgtcccg atatggaaga
    15721 tctaatgcac acaggtagag atcattattg gcatgaatta atggagaatg ttgaaccgag
    15781 tggttacgaa gagttagacg ctacagatcc actctacatt ctatatacat ctggcacaac
    15841 tggcaaaccc aaaggagttg tccgagacaa cggaggtcat gcagttgcaa tgaaacttag
    15901 catggaagcg gtttacgacg ttaagccgcg agatgtattt tgggcggcat ccgatgtagg
    15961 ttgggttgta ggacattctt atattgtgta tgcacctcta ctctatggtt gcactacaat
    16021 tttatatgaa ggaaaacccg ttaagactcc tgatgccgga gcattctgga gagtcatttc
    16081 tgaacataaa gtaaatgtat tattcactgc tccaacggcg ttacgcgcaa ttcgaaaaga
    16141 agatccaaat ggagaactaa aaagtaaata cgatctacga agtcttaaat atctatttct
    16201 tgccggagaa agaacagatc ccccaactta tgaatgggca aaagatttac tcgaaatccc
    16261 agtcattgat cattggtggc agactgagac aggctatgct atttgtggaa atctcatggg
    16321 agttgagcct gttatttcta aagctggttc tgcaacaaga cccatgtttg gatttgatgt
    16381 caagatttta gatgaagaag gttccgaagt aaaaaggggt caaaaaggaa atatcgtaat
    16441 caaattgcct cttcccccag gttgtctgcc aactctttgg aaagattctg aaaattttaa
    16501 gaagtcttat ttgagtcatt atgaaggtta ttacctgact ggtgatggtg gatatttcga
    16561 tgaagatggc tatcttttta tcatgggtag aattgacgat gtgatcaatg tagctggtca
    16621 tagactatcg acaggtgaaa tggaggaaat acttgcctcc catccaactg ttgcagaatg
    16681 tgcagtgatt ggaattgaag atgcgatgaa aggacaagtt cctattggct ttgttgttct
    16741 aaaagatgga gccaagcaag atctagcaga aatagaaaag gagctaattc atcttatccg
    16801 cgaagaaatc ggagcgattg caacatttaa aaatgtttta ttagtaaagc gacttcctaa
    16861 aactagatcc ggaaaaattc ttagaaagac aatgagaaag attgcggatg gcgaagaata
    16921 cactgtccct tccaccatcg acgatccaac aatcctagat gaaatcattg aatatttaag
    16981 agaagcaaaa cttggcaagg catttcaaaa ataaatattt tattaaccaa ggatagaagt
    17041 atgaatagat ggttttatgt tttaatttgc ggattaatca atgtttgtat tgggaatgtt
    17101 tatgcatgga gtgtatttag gaaaccagct gaaaaattct ttagtataac ttctaaagaa
    17161 agtggtttac cttttatgat ctttttggcg ttttttgcgg tgagtatgcc gttaaccggt
    17221 aaattcttag ataaatactc tcctcgtttc tttgctctag ttggaggaac tgtattctca
    17281 ataggttata tcctttctgg atttatggaa aatattcaac aagttgtcct aacgtatggt
    17341 gtaatctctg gactcggggt aggagtaatt tatggaacaa caattgcagt tccagctaaa
    17401 tgggttcctg aaagaaaggg acttgctgtg ggtctatcga ttctgggctt cggagtctct
    17461 cctttgataa cttcctattt aagccattct atgatcgaat cttatggttt aatgaatact
    17521 ttcaaaatct taggtttagt ctacatggtt atcattttaa ttctctctac ttttctaaaa
    17581 tttccaaatg atactcagca acaagcgtta gctcaacagc tagattcttc ttcgattagc
    17641 ccgggagcaa ttgtaaaagg taaatatttt tatgcacttt ggatttgctt ttttataggt
    17701 gcattcacag gtctttctac tatctccata tcaagtccct ttctaataga atcgactgga
    17761 attgcagcag gtgaagcagc actttacgtt tctttcctag caattttcaa tggtttaggc
    17821 agaccattct tcggctttct aacagataaa attggttttc caaaatcaat cctagtctcc
    17881 tacttcgtaa ttattttatc tagtataatc ttatttctga atttcaatca tcttatattc
    17941 ctttatgttt tagcattggc tttactttgg tcgtccttgg gtggatggct tgccattgcg
    18001 cctaccgcta ctgcttcttt attcggaacg aaatattata gtagtaatta tggattaata
    18061 tttaccgcct atggaacgag tgcattgctc ggagtttatc tatctagttc cattcgagat
    18121 ctgtttgggc aatatttatc ttttttttac cctgtgatcg gactttgtat tctcggaata
    18181 ctaatatcat tatttacttt taagaaatga aacctttact cacaaaaatt ttaaatgttt
    18241 cacagatcag aagtttagat gcattcacta tagaaaacga acctgtagca tctatcgatt
    18301 taatggaaag agcttctatt gcttttgtaa aatggtttac agctcatttt gattctgaat
    18361 tttcaattcg aatttttgtg ggacctggaa acaatggagg agatggactt gccattggta
    18421 gacttctttc tcaagaaaaa tacaaagtag aaatttatat cccaacctac gatcaggata
    18481 gatctaaaga ttttttgata aactacaacc gcttgcctca taatgtaaaa agagttgaaa
    18541 tcaactctac aaaggaaatt gaaccggcaa aaaataatac gatcataatt gatgcccttc
    18601 tagggtcagg tctaaataga aaacccaaag gcatcatcga agacgttatt tcttatataa
    18661 accaaagcgg attcattata atatcagtag atattgcctc tggacttttt gcagataagc
    18721 acacagatca ttcttctatt gtaaatccta actatacaat cagttttcaa attcccaaat
    18781 tggctttttt ctttccagat aatagcaagt atgtaggaaa ttggcaactc gtagacattg
    18841 gactttccaa aaaattcatc gaagaagaaa aaacagattc ttatttttta aataaaaatt
    18901 tcctcaaatc tatttacaaa aggagggatc cttttgctca taaaggaaat tacggcaaag
    18961 cactgttagt ctctggatct tatggaaaga tgggagcggc aattcttgcg gcgagaggat
    19021 gtttgcgatc tggtgttggt cttctcactg tccaagtccc caaaaaggga gtggaaattt
    19081 tacaaatatc agttccagaa gctatggtag tgagtggtaa aagaaaaaaa actatttctg
    19141 aaatagattg gaaagaattc gatatagatg caattgcaat tggtccggga attggaaaag
    19201 aagaccagac actgaaaaca ttaacctccc ttcttacaga agtaaaaaaa ccaatggttc
    19261 tagatgcaga cgcgctcaat ctaatctctg agaatccgaa attattggat ctaattcaac
    19321 caaatacaat cttaactcct catttcaaag aatttgaaag gctcgttgga aaatcagaaa
    19381 atgactttca tcgaatttct cttctcagaa attttgcaaa ggataaaaag atttttattg
    19441 ttttaaaggg aaaacacact gccattgcca ctccagaggg agatgtttac tttaatggaa
    19501 ctggaaatcc cggaatggca acaggtggga gtggggatgt gctcacaggt gtaattttat
    19561 cattgcttgc ccaaggatat gaaccgttac acgcaagcct tttcggtgtc tatttacacg
    19621 gactagcggg cgacattgct gcaaaaaaat tcggagaaga aagtcttctt ccatccgatt
    19681 taatcaatta tctaggaaat gcatttttac aaataaataa ttcctattaa acattacttt
    19741 acgcgatata aatttttcca tgattattga attttaaata ataggggttt ggggacgata
    19801 gtccccaagc tgccggcagg ctccctgcca aataactcac ctagcacgaa gtgcgttagg
    19861 tgagttatta aaaataaaaa ctttgatctt ttgtgtttgg aatttttact tctgtgttgc
    19921 caggagtaaa cccgtccata gaaattggat ctgtctttgg taatgtgatc gttgtagttt
    19981 ttgtttttgg ttttcctttt ggttctccat tttcatcaca ggcttgtatt tcattactaa
    20041 cgtttacttt tccagtagat aagttatagt catatgtatt cgaaatttca caaggagcaa
    20101 agaaactagt cgttgctcca atcaaaaacc aaccatcctt ctgaaatcta tatcgatgtg
    20161 tataatgcca tttttctctt gcgccgccga agtgatttac tactatcgca ttgttttcca
    20221 ctttcacatc ttcaaacgga tctcccagca ttccacctgc tctgcttgga agaattgcat
    20281 caatattctt atataacaac tcccactttt catctttctt tttaaaaatt cgaatttccc
    20341 tttcaaagcc taaatcggaa tccttacttg tctcataaat gataacaagt tcctcctctt
    20401 tgtctccatc taaatcacct ctcacttcaa aaagatttct tagccctctg ggagaaggaa
    20461 tggttggatt tgcaatttct tctcttctcg gaattccctc ttcttctgca ataggatcgg
    20521 agtcaaccga ggcaactggc tcttctgttg ttttttcttc ttctttcttt ttacaatcca
    20581 caaaaattag agctaatact aaaacaattg gaacgatcca tttcatacaa tttctccgta
    20641 aaataattca agttgtaatt ttctattata caaatcctac tcgattgtaa agattttttc
    20701 tgaaatgaaa atactataaa ttttgttaga attttttagg gatgctattt ttggatttga
    20761 tagaatgggg tatttattgc ggataacgct tatttaaata gagatttgtt aaaaatcttt
    20821 cagcttcttt acaaaataat ggcgattaat aagagttata attttgaacc tcttcttttt
    20881 ttgctttcaa taaaaattat ttaatactct gtagatgctc agtagaagtt gttgggtata
    20941 cagtgcaacc actacataca aagaaagagt aattcgcttt ctccaataaa tcaattagta
    21001 aaaaatcatc gctttgtaat ttgtatcctt gtaaatctgc gtaaggaagt cttcttgcgt
    21061 ttctccacct aactctcatt cttcgctaaa acaatttgaa ccgcatacac atacttggat
    21121 ttatgtaaaa tagaaaatcc atctttttta tttaaaagtt tattgatttg aattctgcct
    21181 ctttcctcgt tgctattaaa aatcaactcg cctgtttttt cattgtatcc tacaattaaa
    21241 ataaaatgat cacataccca ctttggattt tcatccgggt aaattttaac tccaagtaaa
    21301 attggatttc cttttttaac attttgaatt actttttctt ctaaatattt tctatgatta
    21361 tttacggtag ttgaaatgtt ttgaaaggga atttttaatt tctttaaaac ttttaaaact
    21421 tcgttcgagt gaattccacg tccgggtgag cctgcaagag aattgatttc ttcttgtgtg
    21481 attttcatct ttaaactttt cgcaatcgaa taaatacagg cttctccaca gcttccttct
    21541 ggaatttccc aatattcttt gggtggattt aaattttcta gaatgaaatc accttcttgt
    21601 ttttcaaagg ctagtagcgg caaaatgaga gtaaacgaca aaatgattct tagcatgata
    21661 aagataattg tataaaaatt tcttttctat tgacaattca ttttcaattc gcaatgcttg
    21721 gaatatgaaa tttaaaatat tccttttagt ctttttatgc ctttcaaact tatctgcaga
    21781 ggaactacca gaatcaattc cgaatacact atctattgaa tattatcaaa gtcttggtca
    21841 tgcaggacgt ggattttcaa tggagttaaa ggaaaataac tgcaaatact ctgataaatt
    21901 tatcaatact tccaatgatt tttcctttca actttcaaaa gaggattgga aagaactcta
    21961 tcatatttta aggaaaaaca aagttcataa aattactacc agtagtgaaa tgatacatga
    22021 ctacgatggg ttatctcttt ccattcaatg ggaaaataaa tcgattcaaa tttcgcaaag
    22081 tggtagtaga attagttcca attgggaaaa agaatggaat gctattacaa gtcacttaga
    22141 aaaaattcgt tcaaatgaat accaaaaagc agtcaagtca gtagaaataa gtcttgataa
    22201 atcgttagtc gggaacttca ttcacataca agccaattct caaggtgttg tatttagaaa
    22261 atatgtagaa ataaaaaaca tttcagaaga taaaatccaa ttaaaattcc ttccgggcaa
    22321 acatcgtatc tatgcaatcc agtatgaggg atatgatcct aaaaaattcg aagaagctaa
    22381 ggcaaattca atgtatccag acaatgcgtt aaatccatat ttagaaactt taaaggcaaa
    22441 ccattttact ttgacaattg aaacaattca gaaaggttct tttatcattc gaatgaaaga
    22501 taaaaaacta gttgtgaact aaaagcttac tacttctact ttatacatct tgaagtcttt
    22561 tacattttta gtaactaact ttaaggattg agtgtgtgcg atagaagcga tcattccatc
    22621 tacaaagctt ggagtctttc ctgctttaat cagttttgct ctttcttttg catgccagac
    22681 ggaggcttct ggcgtataat caaaaatagg aattgtatcg cgtatcgtat gaatataact
    22741 ttctaaaact tttttctttt tcccatcttc taggctttcc ataccaaatt gcaattcatg
    22801 caaagcagga ctgcaagtag caattacatc ttcgtttttt tctaaaaatt ctattacatc
    22861 taaatcaggc tttggtttta attgttccga tagaatgttt gtatccagta gataaacaaa
    22921 actcatttaa aatttaaact ctcttccagt cgttttgtct cttagatttg caaaatcttt
    22981 ttctgaaaat gaaacttttt taaattcctc agactctctt aattgtttgc atgcactcaa
    23041 aaaactttta cgtttccctt ttagcaattc atattcacga gtagaaagta aatacgcaac
    23101 tactttgcct cttcttgtaa tagaaatcac tgattgattc tcaagagagt gaactaaaac
    23161 aggaagatta tttttagtat gtgctatgct gtaacttgct tccgtgagta ataccatata
    23221 tccatctaga tagctatttc aaggatatgt caaccctttt tcaaaaaaaa tctttttcca
    23281 ataatgagtt ttttaattgc tagtttcatt ctatagcaga aatcagaacc atgtgcattt
    23341 tatttattgc aatcaacgaa cataaaaact ttcctctgat cattctcgca aatagagatg
    23401 aatttcagaa tagacccaca agatcagctc agttttggga agaccatcca aacttacttg
    23461 caggaagaga tgaacaagac aaaggaactt ggcttggaat cacaaaagag aaacgaattt
    23521 ccgcactcac aaattacaga aatattccgc ttcacaaaga aggtagaatt tctcgcggac
    23581 tcttagtgcg agattttctt atatcaaaca aaactgcaga agaataccta gaaattttag
    23641 aatcatcaaa ggaaaattac aatccttaca atatcttact cggaaatcca actgaacttt
    23701 atgtgtataa taacgtcagt aaccaaagac aaaaattaga aaaaggattt catggactca
    23761 gcaatgcatt cctaaattct ccttggccaa aactttctcg tggaattgag catttaaaaa
    23821 aagcagtttt gactaatcaa ttagagaaag actatctttt ttctctcatg caagatgaaa
    23881 caaaggctac tgatgattta cttccagaca caggagttgg actcgataaa gaaagatttc
    23941 tatcatccat tttcctaaag agtgaagtgt atggaactag atcgactgct atccttctct
    24001 ttgatcggca aaatactgtt agcttctacg aaaaaactta taatcctaaa ggagaagtag
    24061 taaaagaatt tagtgcaagt ctataatcat atttgaaaat aaaattaaaa ctcattttat
    24121 ctttgtttca tctttttaga atttgagata gttttctttt tttatttcta atttcatttt
    24181 taattgtaga atcgataaac tttatttttc atggaatcag tttcagatga aatgaggtat
    24241 actatgaaag ttggaatttt aggttctgga gttgtgggac aaacattagc aaaaggtttt
    24301 ttaaaacatg gctatgaagt tatgattgga agtagagatg tgtcaaaact ttctgacttc
    24361 gttaaagaaa atagtaatat aaagtccgga aatttttcag agactgcatc attcggagat
    24421 ataatcgttc tcactgttaa aggaaatgtt gcgatagacg ctataaatct cgctggcgtt
    24481 aaaaacctag aaggaaaaac catcatcgat acaatgaatc caataggaga caaacctcca
    24541 gtaaatgggg ttttaaattt tttcacagac ttaaaccaat ctcttatgga aaaactacaa
    24601 accagtttcc ccaatttaaa atttgtaaaa gcatttagtt gtataggtgc tcccttcatg
    24661 gtaaatccta atttcagtgg aggaaaacca tcgatgttta tttgcggaaa tgatgaaaat
    24721 gccaagaaag atgttagtaa aattttagat caattcggat ttgaaacaga agacatgggc
    24781 aaagtagaag cagcaagagc cattgaacca ctttgtattc tctggtgtat tccggggatg
    24841 tcgcaaaaca aatgggcaca tgcatttaaa cttttgaaac tttagttcaa aattattctt
    24901 ttataaaaag atgaatagcc ttctagatac gttatactct acagttgtaa atcgggattt
    24961 attttttcaa tccggatggg gcgacaaaca tggattagaa tatttattat cagtgaatcc
    25021 tacatctatt catctgcgtc ctgtaaaaga aatcgatata aaatggggag aaataaaaaa
    25081 agagaaagaa tatttagttc aggatggaga atttcaaagt ccatttgaat ttatctatga
    25141 tatcaaggga gaaaaagtaa aagttgaatt gccagaagaa acgaaaactg cttacatcca
    25201 aagaattctt cctttcgatt ttagccttca gtcacctctg actattcatt ttgcagcaac
    25261 aggagacgag ggttattcta gacgtcgtct aatgctcgct atccccttag ccaaaaaagg
    25321 aataggttct gtattactag aagatcctta ttatggcaaa cgacgaccaa aaggacaaaa
    25381 aggcgttgta attcgcacat tcactgaatt tcttagaatg tcgagagctg ccacagatga
    25441 aggcattgcg cttctcaaat attttcatag agccggtcat agaaagttag gcgtaacagg
    25501 aattagtatg ggaggatacg tatcattaac cgcttcggct cgatccaatc tagaaattgc
    25561 agttgcggct tgtattccaa gtcattctgg ttcacctgtt tacgtagaag gtgctttgag
    25621 taaagcttgt gattggacga gcctttcttc tccatttcaa aatccaattg atgcaaaaca
    25681 ttttatgcgt ttaattctag accaatccga tatccggagt tttccaaaac caaaaagaat
    25741 ggatgcagtc atcattgtgg gcgcaaagaa agatggctat attcccaatt actccactca
    25801 aatcatcaaa gatcattggg aaaaagcaga actacgatgg gtaaaagcag ggcatgtagg
    25861 ttccttcctt ttttgcactc ccgattttag aaaagcaata gaagactctt ttagtcgact
    25921 tgtatccaat gaattctatt caaatcctat cgttacaaat ctatagattc taacaggctt
    25981 acagcagcag ggaaaatagg caccgtgatt tttatagaat aaggtttaat ctaattttct
    26041 attcaatcaa tacaaatatt tcgaattaaa tcacaacgat agctgaagtc taaatccagc
    26101 ctttttatct ctcttttaac tgttttatat gcccaagcat ggaatctatc tcttatctaa
    26161 actagattat agaatcagcg agtggcagtc tttttaaaat gcattcaaag taaatgactc
    26221 catttcgaga aatcagtttt tatcaaacgg tttaaaaatc tattgccaaa aatctctttg
    26281 aatttgagac ttatccctaa tttccaaaaa cgaagactct actaaaagat ctgaattttt
    26341 gctgcaaacc tattttctaa aatcatctcg aaaggactga actatcttgt gtataacttg
    26401 tttgaatcta acgttagcaa acaactcttt agcgaaagat ggaattttct gatacccaag
    26461 cgattgctat cgatagagaa gaatagaatt ttgggaaaaa agatttaggg aacagctaac
    26521 ttgatccagt tgagttcaaa atggaaaact aaagaataga aatttaaaaa agagggaaaa
    26581 atgattatag agcattcact agacaattca aaaatcacag aagatgagga aaagaaaaaa
    26641 gttaaaacaa aatcggatgt aatactcatt ggagcaggga ttatgagtgc aaccctcgga
    26701 gtctttctaa aagaactaga acctgatcta acaatcacag tcctagaaag attagacaat
    26761 gctgccagag aaagttcaaa cgcttggaat aatgctggca caggtcactc tgccttttgt
    26821 gaattaaact ataccctcga aaatgaagat ggctcaatcg atattaaaaa agctttgaaa
    26881 atcgcctccg cgtttgaagt atcaaaagag ttttggtcct atcttgtaaa agaaaaaaag
    26941 attatcgagc cagaacagtt tatccatgca attcctcatt atagttttgt atggggtgag
    27001 gaagatgttg attttctaaa aagacgttat gacgcaatga tcaaagaacc aatctttaaa
    27061 gatttggaat actcggaaga aagaaaggtc ctatttaagt ggatgccttt ggtattaaaa
    27121 ggtagagacc ctatggcaaa agtagccgga acaaaaatgg ctgttggagt agacgtagac
    27181 tttggaactt taaccagagc tatgttcaag tatctctcta cattagaagg agttacagtc
    27241 aaataccacg aggacgtaaa agatttagtt cgagagaaag atggtctttg gaatttaacc
    27301 gcaacaaatc agctaacgca tgaaatagag catcacgaag cgagctatgt ttttattggg
    27361 gctggtggtg gcgcattacc cttacttgag aaatctgaaa ttcctgaagc aaaaggtttt
    27421 ggaggatttc caatcagtgg gcaatggctt cgatgtgtaa atccagaagt gatcgatact
    27481 cattttgcaa aagtatatgg aaaagcgtca gtcggttcac ctcctatgtc tgttccacat
    27541 ttagatacaa gaataatcaa tggcaaaaaa gaattactct ttggtcccta tgcaggattc
    27601 actactcgat tcttaaagaa aggctcttat tgggatttgg ccaaatcact cgagcttgga
    27661 aatatttttc caatgatctc tgcgggtatg cacaatttgc ctctcactaa atatttaatt
    27721 acacaagctc tccaatctca tagagataga atgaaatctc taaaagctta ctttccaaaa
    27781 gcaaaaagta aagactggaa attagaaatt gcaggcaaac gagtgcaggt aattaaagcc
    27841 gatgaaaaag aaggaggagt tttagaattt ggaacagaag ttgtagccgc taatgatgga
    27901 acgttagcag ccctactagg agcttctcct ggtgcttcca cctcggttgc aatcatgttg
    27961 gaagttttag cagattgttt tactagaaaa actgcaacgc cagagtggca agccaaacta
    28021 aaaaaaatga tccctagttt cggagaatct ttcattcaaa acgaagaatt agtaaaaaga
    28081 agtagatcta tgacggaaga gattttaaag ttgaaattag attaagagga taataatgat
    28141 aagtcgcatc ccttggaaat ccaagggata ttcactttag tgttttgcgc gtttgtcgtg
    28201 gtggtgactg agttggtgtt ctaacttgtc ttcgattcca tcaatacaag catgaatttc
    28261 cttgtctgtg ttgtgagcat taaagtctgc tttatcagca tgcatgttga aaactgcggt
    28321 aatattgccg tgcactttct caaagctcaa ttcagccttg gttacttcat ggacatattt
    28381 cgaaagtcta cttaatttgt cattggcata gctctccaca gcctcagaac gatctaaatg
    28441 cttccaagta aattttattt tcatttttat atcctcgtta gatatgtttt agagtaaggc
    28501 tagaaggtca acaaaaaagt tcaagtttgg actactgtga tagatactat taacgcgaat
    28561 tcgatgtaaa aatattttca ccaccgactt gcactgagcc atgtcgaatg tgtgcacacc
    28621 gataactgaa cgatgaaaac acgatggata attttgaatt atcgtatctt ttatcagtga
    28681 tattcgtccc tccacccaaa atccattacc gctcattcgc ggtaataggt agtaatcttt
    28741 taatttttct gctacatcga attggcgtta ttagtgaaat tgccgatgag ataaaatttt
    28801 atcgtcatat attttcttgt cactttccgt tatatttttt ggataccgcg cataactttt
    28861 ttctcgacga ttaagccaat ctcttccaag taatttttct ctttctattt ctaaatcagg
    28921 aagatttgta taggcaatcg ttgtttcttc tttaggtcct gttccaactc cccttcctac
    28981 ccaaaatcca ctgtataata aggcagaccg tatagcggta gacgcactat aagtataaag
    29041 tcgagtaatt tgatttggtt tgtatgtgta ttgaaagatt tccgaaaaga cttcacttga
    29101 ccaaagaatg tcatctactt taaaagaaaa aggatcataa aaaataagat ctggaatttt
    29161 ttcttttgaa aactcttttc taaaatcccc atgaatgagt ttccatgtaa ataaatcttt
    29221 ttcccaaaac ttttcttcta aaattttctt tggagcttta tgaaataaat gaggaaatct
    29281 tcctgaattt cttagagcaa gtttcaatgg atctaaatct atctcaaaac taatcaactc
    29341 tactttcctt gcactatgaa tagactcaaa acaagataga accgccatag cattgtgacc
    29401 agctccaagc cctacatccc aaatcacaag aggatcttca tggctaatat ccattagccg
    29461 ctcttttaga ttggattggt ctatgtaaag tctctttgct tcttctttcg gatcagaaac
    29521 agaatgcatg acttccccag aacttttttg ccttacactc caaaatcctt ctttattttg
    29581 aatgatctca taatccccta attgcttttt cttatctaga gaactgattt tgatatcagg
    29641 cggatttaca ctatcgcttt gtaaaagctc tactttttta gttctataga aggataaaaa
    29701 tgtattttta aaaatagctt cgcgcatact agccattagc ctgtgataat aagttaaatt
    29761 atgaattgcc atcagatgcc aacccaagaa ttcatttgcc ttataaagat gatgtaaata
    29821 cgctctagaa taatttttac agcagacaca gtcacaactt ggatctagaa ctgtatcatc
    29881 taacttatat ttacctcttc tcatttgtaa tttaccaagg cttgtaaacg caacagatct
    29941 ctgtgcttgc tccaccggta gaatacaatc aaacatatct acccctctat gcaccgcttc
    30001 aagtagatca attggagttc caacacccat taaataacgc ggcatatttt taggtagaag
    30061 ggaagcagat agttcacaaa agtcatttcg ttcttctttt gtctctccaa ctgcaagtcc
    30121 accaattgca aacccatcga agggcatgtc tgtaatttgc gcggcgctct cttttcttaa
    30181 atcagtaaaa caagcccctt gtataattcc aaataaagct tgattggaat ctcctcttgc
    30241 aactaggctt ctcttcgccc agcgatgcgt tagctccatc gcttcccttg ccatattata
    30301 atcagaagta gaaggaacac attcatctaa aaccatcata atatcagaac cgattgcctt
    30361 ttgtgtttca atacttagct caggagaaag taaaatattt cttccgtcaa tgtaacttct
    30421 aaaaacggct ccctcctcag tgatactcct tgtattttct agactgaaga tttgaaatcc
    30481 acctgaatct gtaagaactg gcttggtcca attcataaac ttatgaatcc caccgattct
    30541 ttgaaatact tcaattccag gtcgaagtag aagatgataa gtatttgcaa gtaaaatatt
    30601 ggaacctgta gtctctaacg tatcttttgt ttgagacttg actgtcgctt gtgttccaac
    30661 aggcataaag ataggagtta aaacttctcc atgtaaagtc ataaatcgag cagctcttgc
    30721 tttgctattt tctgcttctt tctctaattg aaaattgagt cgggacattt aattcatcat
    30781 ctagaaataa tttttaattc atgaaataat acaggatttg ttttcatgat atataaaatt
    30841 ctttgtgcag gaccttgtgg aatatttcta ccagactccc atgcttctac tgttttttta
    30901 gatacaccaa gagctttcgc aaataaattt tgagaaagat ttattttctt tcgtatgatt
    30961 ttaatttctt ccttttgata gtttgctaaa ggtaaaatag aaaatcgatg ttctacactt
    31021 tgttttttat ttccttttgc atgcgcaact gcttgattta aacctcttaa aatacttgca
    31081 gttacttctc tagaagattt gtttttagta attacttttt tcatagaatg aacctccttt
    31141 attgtgtttt cttcaataca ttatctattg tttctataat tttagaaatt gttcttttat
    31201 cttctgcaga gatattttcc gtttcatttt tcttcaataa agaaataaaa tacaagacct
    31261 catgcaatgg ataatctaca tataaaattc gaatgccacc actctttcct ttccctggca
    31321 aagcccatct catttttctg agtccaccag tgccagaaat aacttttcca tatttaggat
    31381 ttgtaattaa aaaagtttct acctctctta aatctccatc atctagccca attgatttcc
    31441 atagagtatc aaattctttt aatgaaacaa agactctggt cacaaaaata tagtatccta
    31501 cctagtaggg taatgtcaaa tgaaaaaatt ttcattagaa tctaaagacc ttttttcact
    31561 agaaattatg cctatcctag aaaacttgga gattatgatt aaagtttcga atgtgtataa
    31621 atcttttgga agtcaggagc tttttgaaga cctgagcttt gccgttaacc gaggagaaaa
    31681 agttggactc gttggtagaa atggtcatgg taaatcgaca ttatttcaaa tgcttcttgg
    31741 aaatctagaa ccagataaag gtgttataac tgttcctaag aattataaaa ttggtcattt
    31801 gcaacaacat ttaaaattca caaaacctac agttctagaa gaatgcgctc tcggtcttcc
    31861 agaaggggaa gaatacgaaa cttggcaggt ggaaagaatt ctatcaggtc ttggattctc
    31921 tgaaaaagat atggaaagaa gcccagatga attttcagga ggatatcaaa ttagaatgaa
    31981 tctagcaaag cttttggttt ctagaccaga catgcttatg ttagatgagc caaataacta
    32041 tctagatatt gttacgattc gttggctcga agaattcttg cgagaatggg aaggagaaat
    32101 aatccttgtt acccacgata gaagttttat ggatgcagtg gtttcgcatg tcgttgccat
    32161 tcaccgaaaa aaagcaatta agacagaagg tgatactgaa aaactttaca atcaaatcaa
    32221 tcaagcagaa gaaatttacg aaaagactcg actcaatgaa gcaaaaaaac gaaaacaaga
    32281 agaagtgttt atcgcacgtt ttaaagcaaa agccagcttt gccagtagaa ctcaatctcg
    32341 tgttaaaaaa ttagaaaaac agggagagat gaaagcactc gaagcaattg aaacacttga
    32401 gctttatttc aatgcagctc ctttttcagc aagtcaaatg ctatccgcag atgaaatcaa
    32461 attttcctat acaggaaatc ctccttactt agtagaaaat ttttctatca gtgtaggcaa
    32521 acaagataga atttgcatca ttggaaaaaa tggaaaaggt aaatctactc tactcaaatt
    32581 acttgctggc gagttagaac cgttatccgg taagatcaat aaacatcctg ttttaaaaga
    32641 aggatatttt ggccaaacga ataaactcaa catggaggaa agtaacactg tagtcgaaga
    32701 aattaaaagt gcagataaat cttgcacgga aaacttagca agaagtatcg ccggaggtct
    32761 tatgttctca gaagatgctg ccttaaaaaa aataaaggta ttatcgggag gagaaaaaag
    32821 tagagtgctt ctcgggaaaa tccttgttac cccctgcaat ttacttttct tagatgagcc
    32881 aacaaaccac ttagatatgc aatcctgtga ttcactcatt gaagccatcg atcaatttga
    32941 tggaagtgta atcatggtaa ctcacaacga gatgcatcta agagcagttg cgactaagct
    33001 tattgttttt gataaagata aaatctctgt ttatgatggc ggttacgatg acttcttatc
    33061 tgatgtaggt tggtcagacg agtctcttta gaaaaatgct ttacaaatca tgacatgtat
    33121 aacacaatgt tatacatgtt agcattacga ctacctattg cgattgaaga gaaattagac
    33181 gaaatctcga aagcagagaa aaaaacaaaa tcccaactgc ttcgagaagc gatcgagcta
    33241 tactttgatg cttaccaaaa aaaaacctct gcctttgaat taggaaaaga attctttggt
    33301 aaatatggaa gtggcgtaaa agacaattct gaaaattaca aaaaaaggtt aaaagagaga
    33361 tggaatgcaa agcgctcttc ttgatgcagg acctctgatt gccctctttg ataattcaga
    33421 caagcaccac aagaaagtgt atcatttttt tcagtcctac gttggaaatt tatatacaac
    33481 ttggcctgtg atcacagaaa caatgcattt cctaaatttt aatccaaatg tgcaatctga
    33541 ttttctacaa tggcttcaga gagacccact tcacattcca gaacacaata aagcaatttt
    33601 agaaaaatta agaaagctaa ttctaaaata caaaaatgtt ccgatggact tagcagatgc
    33661 aagtcttgtt ttactatccg aggaaatgaa tatcaaacat atcatcactc tcgattcaga
    33721 ttatgcaatt tataaaatga atcgaaagtc ttttaaaagt ttgctttaaa aaattccctg
    33781 ccatttgagt agacccgcaa ttcctagtat cggcattcca taaaatatat tctcgggagt
    33841 cttagagaga gcctctaaat aggaagcatt gtctaatgga attttaaaat ttgtctctga
    33901 cttataacgt ctttccaatt cttgaaataa tacaggccaa ccagaaggca gaatatataa
    33961 aattttatct agattgtctc tccatatatc gcctaactct tcgagcatat aaggatcttt
    34021 tccataaatt tccacagcaa gatctaattc tgtctgcatt tgaaattgaa cagagtctct
    34081 taatgcatga atttcttttt tcttgagttt ctctaagggc ttatcggtca tctcataaag
    34141 tttaattgtc tttcctcggt aataagtaag ctttgatggc attccaaagt aataaaccca
    34201 cggttgtaaa gcgactaacg gtgtcagtgg ccccattgga agaaatggaa ttcctagttt
    34261 attaacaagt ttatttagat catcattttt ataactataa ggattgatat attcagcatt
    34321 tacaatcgaa acaggaatga tatcagtttt atatttaatt gccatacgaa taaaagaact
    34381 ggaaaattgt tgcagttgat atttcttatc aaagcccttt ccgatgcctg caattccttc
    34441 tggaaaaatt aaaacattcg catctttgat ttgcatcata gtatcgaagt tatcaagcgt
    34501 tgcatccact ccccccaaac gtttccagaa attttctata aagtaaggtt gcatataacg
    34561 tgaggcagat aaagcaggtg ctgtgagtgc gcgtatcgaa tttttcattt gaaacttatt
    34621 ttgttgaaag actctacctg caaaaataat tgcatcccaa ggaaaaccca ttcctgaatg
    34681 attacatata taaatggctg gagcctctgg attatttctt tccggaatag tttcgaaatt
    34741 tatcattcta gatctaaaat agtatctatc gagaggatct aaaatattag tagccattgc
    34801 ctttaaataa tctagatcaa aatggcgatt aatggattct aggttagaaa aatttgaacc
    34861 gttattcgaa agagtttttt taaaattaga atccatttcc tagtccttga gatagttaaa
    34921 ttttatatat tcaaaatcat tttgagatat ttcttcttta aaggcaatat ttaagatatt
    34981 agaaaaatga agattctttc caatttttaa ataatgcttt ctattttcag agagtggttt
    35041 agctgtttta acaactccta ccctttcgaa atttttgtct ttgacctgaa aatgaattgt
    35101 gacttgatct cccattttgt gaggaagcac agataaaacc atcatcccac cagttccaag
    35161 gttttgtata taacagtctt tttcctgttc cccttttgtg tttttaaaat cacaaaaagc
    35221 ttctaacgta agtctttgtg cttttctttt ttcagagaat tcttgcggca taaattaatc
    35281 ctactttaaa tttggaaata ccaactccaa cctcaattct tgttataaat tttgcaagat
    35341 gaaaagtttt ttatcatgaa tttcatttct tgataaacag atttggaatt gtttgtttga
    35401 ctaataacat tagtttaaaa acaattgtgc ttacttgctc atttcacttt ttaattttat
    35461 atgagtaagc tactattgat gaattagaaa ctatttctca ttctctttag aatactgaat
    35521 tcatttggaa aataatatga aacatttact ccttactgtc ccatttttga ttctaagtct
    35581 attttgctcg agtgttcaaa aagtagaccc aaaagctgat ttagtaaatg gatttactcc
    35641 tattaaaaat gatgaactag caaaacgata tgcgccatta ttaaaggctg gttcaatcta
    35701 tggagatcca tttgccctct attatagagc aagtaaagat gatgcaggaa atacacatat
    35761 aacctaccac ttcgcttggg aaaaagaaga aaatacaact tctggaattg gtccattctt
    35821 tagtcgcaat atttacactg gtgggcttaa attacaaaag acgatgttcg gaaaaggaga
    35881 cttagaaatg gtttctttaa tcatcgattc aaatggagtc attcaacaag ttgaatatga
    35941 aattgcagaa aattatgatc cgagtaaatt tggagtaaaa cacaaacacg tcatacgcac
    36001 agaaccacat aaagaattac tcgcttttga agtaatgtca tggaatcatt tattccaata
    36061 cttaggagat aaaaatgaat cttctcaaaa ctttctgcaa cttaccccta cttatttcac
    36121 acaaagttta tgggaagaat acaccatgat aaaagaaaaa gaaggtttct tcagtagaaa
    36181 cagagctcat aaaccctatg aagttgagta tatagaataa agtttgcgat gtctctgtta
    36241 aaagaaaaag gaattgtagt tgcaacaaga caggtagggg aagcagatgc ggtcatcaca
    36301 attctaggtg agtccggaac gaaagccaag tataaattaa aaggtattaa aaaaagtaaa
    36361 aatagaccta ttcttgcaag tgaattaggt gcgttaatcg cgattgacta ctatcaacat
    36421 aaaaatgagg aaatccataa tatcaaagaa gtaagtttga tagaaagatt cgagaagata
    36481 aaagagactt acgagggtta tctactcata acttattttt gtgaactaat ggatactatt
    36541 ttaccaaatg gagatagcca tgaaaaatgc tttgagttat tgaatgcagc atttcatgca
    36601 atcaatgaag aaaattttca acctctcatc ttgccatttt tcaaattaaa attattatca
    36661 gtgcttggac ttgtctcaag ggaattaatc tgtcattctt gtgaggaaga tatgttgctt
    36721 aaaaatgcag cgagtatcaa ttcaattcat ttagacatta cgtgtggaga ttgtatgccc
    36781 ccatctaaaa atcagatttc aatcattaaa cttatggatc gaatctttaa atctagatat
    36841 gcaactcttt ccaaagagga aacgccgatt tccctcattt cccaattaga tcaaatttta
    36901 aatgattatt taagagccta tttaaatgtt accttgaaga cttttgattt attttataaa
    36961 tccttaggag gcaaatatga aattcgcaat taaaactgct atagcacttg ctatgatttc
    37021 actgattcta ggttttctac ttaggtatgc agatcgaaat gaatacgaaa tattaaaacc
    37081 agaagtgatc aatacggagg atgatcggtc tagctgggct attattatgg atgtcaaaaa
    37141 ggatatattt aaaaacatag aaatgaattg gcagaagaag cacacgaaat gaaagaaaac
    37201 gaaattttaa aaaacttagt attaaaagaa ttagaaactg ctgtttctaa atttgtatct
    37261 gaatctgata aagataaatt aaaaataaga gtagaatact cccgcaatga aaaatttgga
    37321 gattactcga ctccgttttt aatggaaaat aaagatctgt taggtgatcc aaaggctaat
    37381 agtgataaat tcctaaacct tttcaaagat gttaaattat tttcaaaggt tgattttact
    37441 ccacctggat ttatcaattt taaaattgaa gatcaatttt tactcaatta tattgattct
    37501 tttttaacag cggcaaatgg tccttttgcg caagtagagg aaaaagaaaa aatcattttc
    37561 gaatttgtaa gcgcaaaccc aacaggaccc ttaaatattg tgtctgctcg tgccgcagcc
    37621 acaggggata caatttgcaa tctactttcc acgctaggac atacagtcca tcgagagttt
    37681 tatgtaaatg attatgggaa ccaagttttc cttttaggag tttcttgcct ttcaagactg
    37741 agagaaaatt tatccggtga gactcttacc atccaagaag aaggagatac gactccaatt
    37801 caaacactac tcgaaaaaaa tattctaccc tcggaaggat atagaggaga atatataaaa
    37861 gatgtagcac tcaagattta tgcagaatcg gataaaaaga gcacgatcga tagtctcctt
    37921 aaatccaaag aatacgagaa gctagcagaa aaattttctg tttgggctgt tgaaaaaaat
    37981 ctagaagcac aaaaagaaga cttagtatct tttggagtaa actttgattt atactatagc
    38041 gaaaagtcac tccatgaggc aaacaaagta ttagccgtat tagaagattt aaaaaaagca
    38101 aatgatataa aagaagaaga aggcaaacag gtatttacct caatcaaata tggagatgat
    38161 aaagatagag ttgttgtaag agatgatggg cgaccaactt atcttttagc agatatcgct
    38221 tatcataaat ccaaaatgga tcgaggcttt acacaaatta taaatatttg gggaccggat
    38281 catcacggat acattgcgag acttagagga gctatgattt ctctaggctt tgcagaatct
    38341 aggttcaaag ttctaattgc acaacaagtc aatctaattt caaaaggcga gaagttaaag
    38401 atgagtaaaa gacttggaca attccagaca atgaaagatt tactggaatt tttaggagaa
    38461 agctcaaaag acgttggacg ctatttcttt gtaatgagat ccctcgagtc tcctcttgat
    38521 tttgatctag acttggcaaa agaagaatca gataaaaatc cagtctttta tattcaatat
    38581 gctcatgctc gaattcattc tatattcaga gagacaggca atgaatacag ttttgaaaaa
    38641 ttaaaatcaa tctctcttac agaagaaaga cgatctttac ttttctgggt atctcgattt
    38701 cctgaagaga tactagatgc ggcaaaaaac agagaacctc atagagttac aaattactta
    38761 caaaatctaa gtaaagcatt cacacgtttc tatggagcta aagacaatcg tttgaaagat
    38821 tgtgatgagt ctacccgtct tggactcgct tatatctgcc aagcatctgc caaagcaatc
    38881 aaagaaggat taagtattct aggtattagt tcaccggaaa aaatggaaag gagcgcataa
    38941 tgctaagtat tcacattatt tcaactggga cagaaattac atctggaaaa agtgtagata
    39001 caaattctac atggattgca aatgaattaa caggacttgg gttttcaatc aataaatttc
    39061 tagcacttcc agacaaacca ttgttaatcg aagaagaaat acgttcgatc atggcaagac
    39121 ctggagaaaa tctaatcatc atgactggtg gacttggggc aacagatgat gaccatactt
    39181 taaatgttgt ttgtaaaatc gttgaaaaag atgcagtcac agatcaaaag gctctcgaca
    39241 aactaacgta tttttccgag caaagaggaa aaatgtatca agacctactg cctgtttcca
    39301 aaagacaaac aactattcct ctcggtgctc gtcccttaga caatgatata ggactagcac
    39361 ccggatttta tttagaactc aatactacaa ctagacttgc agcgatgccc ggtgttccaa
    39421 aagagatgaa aaaaatgttc agtgattatt ttcttccttt gatgaaaaaa gattataaca
    39481 aagaagattt gcaaagtcga tctcgctcca tctggggagt tacagaaagt atttttcaag
    39541 caacctttat caaaaaacac caggagttaa tcaacgaagg gctcgaatgg ggagttactg
    39601 caaagccagg acatattaaa gttaccttca aatctacttc ttctgaaaga ttaagaaaaa
    39661 tcatttcatt tctagatact gagtataaag aaaaaatcgg agatgagatt tttctagata
    39721 tccactctat gttaatcagt tctaaaagaa cagtctccac tgccgagagt tgcactggtg
    39781 ggcttgttgg aaaaatttta actgatatgt ctggttcttc tgcttactac ctcggctctg
    39841 tcgttgccta tcataatgat attaagcaaa atattctagg cgtaaaaaaa tcaactctag
    39901 atactgttgg agccgttagt tctgagactg ctattgaaat ggctgaagga gttcagcata
    39961 aattcaacac aaattatgca atctctgtaa ccggtatagc tggaccgaca ggcgcaacaa
    40021 aagataaaaa agtaggtctt gtatatattg gaattaaagc aaatcatgaa gaaacaaaag
    40081 ttttcagata tgagattcca ttgagtagag atatatttag agattatgtt gcgaacattg
    40141 cgctctttca tctctaccaa aaattaaaac aggacaattt ataatactat gctttattat
    40201 tttttcttta caatcattct gtcctttatt atctttttga atttaggaac cccctatact
    40261 caaatgtcac atgtgcagga aaactgggct tggaataaat cccaagagat tggaatgtca
    40321 caagactctc gattcaaaat aaatccattg caaactctga atggatataa aactaaaaat
    40381 ggagtctatt tttttaaagc tgggaaattt tcgctagaag aaaacacata cagtgaattg
    40441 cctctaaatg gaaaaggaca ttttgtttat aaaaaaattg gaagcagtgt cttttattat
    40501 tcaaacgatg gagaaattct ctgggaaaaa ccttataaaa gctatccacg tgtatcgaat
    40561 aacggcgaat tattattatt tatttcggga gatggaaatc aagttctaat ctctgatatc
    40621 aatggaaatg aattcggcgc aaaacaaata gatggcagat tcctatcgga tatttctttt
    40681 ccagcattag taaatggagc gttacttgtt ttctcgggtg gtgagattgc tcttttaaat
    40741 ggaagcggta aaatagtatt tcaaaaaaaa gacgaaccga actcaaagga ctttttattt
    40801 tgtaaaagtg gagccatttc tcctaccggt aaattttcac ttgtgcatta tattagaaac
    40861 acaaaggact ttatttctat actcaatgag aaaggagaga ttgtttctag cataacattg
    40921 gaatcggtat atccgcataa aatttttatg gcagtggatg atgaaggaaa tatggcagtt
    40981 aatttgcccg atcaggtatt tgtcataaat aaaaaaggga aatctgtctt taaaaaagtc
    41041 aaatctagaa aagaagatat ttaccaaatt gcatatacga gtggtagttt ctttgcagtg
    41101 agtttagaaa atcaaatttt atttttgaaa agcgatggtt cttatctagg gaaaagatct
    41161 gttacaatgc cagctagaat gcgaccttcc actgagagag attctgcctt tctagaaaca
    41221 gaagaggaaa ttatctcctt tcgcttattt aaataaattc aaaacatgat agttgagcct
    41281 aaggatcttg ttccattcgc atgcaaggca actggaaaat gttgtattca taatttagtt
    41341 ttactctcca gttttgatat ttttagattg gcaaaatctc tttctcttac agcaaaagat
    41401 ctgtttgaaa aaaaatattt aacatataga attaatcctg tgacgtattg gatgcagcca
    41461 attttaaatg aaaaatcaaa ctctacttgt ccatttcttg tgagagatat agatagcaca
    41521 aatgaaaaat acatttgtga tgtatatgaa gctagaccgt tagtgtgtag actctatcca
    41581 ttaaaatttg ataaagatga aaatattttt atgcgatttc tacctgcaga gtatagatgc
    41641 tatgagtgta taacgacaga gaataatcca accctaaaag aatatttgga ttcctcaaat
    41701 tcttcagagc tcttagagga gtttaaacga tatttagcat tgaaagataa agtcatcgca
    41761 agtggtctaa acttaaatca gatcaaaaaa aataaaccag ctcaatctaa attttttcaa
    41821 attcaatccc tactttatga aacctatcca aatgaagaga accttcacct aactctacca
    41881 tttcaagaaa tagaaaaaaa gattgagcaa attttaaaaa cggaagacta agctatttct
    41941 ttttatcatc ctttttaaaa agagatttta tcttttcttg catttcagga tcttgtgctt
    42001 tttctttcac agtctcgatt actttttttc cttcatctga atctgctttt tcttttagta
    42061 tctctaacat agatttttca ttgctagttg cacttgcttt ttcaggttct acttgtgtat
    42121 ttgtatcggg actggaagaa caataaagtg aaaatagtaa aataaatatt ggaagatttt
    42181 gtttcataaa taactcctat ttgcttctct ctgttccgta gtaaaaatta ccacgtttct
    42241 caatgagatt acgagagatt aaattttctg caatgattaa aagaccagat acagcacttt
    42301 cctttttttc tacttctaca tttttttgtt gtgccataag agagtgaaca tactctcgtc
    42361 tttttccatc caagacccca agcatagaac caactacttt gggatctcca tgcaaaaaag
    42421 caagtgcaag tgtagaagga ggagtttcat tgcagagata aaaaagggca agattatcta
    42481 aatagcttaa atcatgaaat ttagaaaaat gagaattttc tcctgccatt attcctgctc
    42541 ctgcactttt gatttaagat acttaactaa ataatcactc tttggaagtt cacctgtcac
    42601 ttctttaatc aaatcttttg gatctagaat tcttccatga gagtaaacct ttttagaaag
    42661 ccatttattt aaactactaa cgtctcctct cttgagtaga tcatcccaaa aactttcatg
    42721 agttgctaga aagtgatgaa agagttgtgc agaataaata ttcccgagtg tgtaggttgg
    42781 aaaataacca aaggcaccac cactccaatg cacatcttgt aaaacaccct ctccatcatt
    42841 ttcaattttt aaatcaaagc tttctttcat ctttgtattc caaacttcag gaaggtcgga
    42901 aacttttatt tttctagaaa tcaaatctct ttcaatttca aatctgagaa tgatatgaag
    42961 gttgtaggta acctgatctg cctctactcg aatcttagat ttattcacag agtttacgta
    43021 attgtagaaa tcgttaaacg gtagatcata gtgataaata ttgaattctt ttaataaaat
    43081 tggataatag tattcccaaa aggctctaga tcttcccact tgattttccc acaaacggct
    43141 ttgggattca tgcactccaa gagatacagc actagtaaga gggcttgggt aattcgccat
    43201 ttgccccaca ccatactcat agagagcatg tccggtctca tgtaaaactc caaagataga
    43261 agagagagga tcatctacat ggtaacgagt tgtgatccgc ttatcttttg aaccaagact
    43321 tgtagagaat ggatggacac tgatatcaag tctagatgca ctagcgggta atccgagtaa
    43381 tgcgggaagc ttttcattaa aagacttttg taattcaaca ggaattggtt tcttaaaagg
    43441 agaagaataa gacttaccct cattgacgat tggaatgagc gaatttttta aatgtaaaaa
    43501 taattcctct aaatctttcg ctctagctcc tgtttcatat ccttctagaa gtgcatcata
    43561 acgttctgtt gtataaccat aacactcagc catttcaata ctaagttctg tgatttctgt
    43621 aagcgttggt tcaaatgctt tgaagtcttt gttctttttt gcatctgccc agattccatg
    43681 tgctagattt gtgagtttag aaaatcgctc tactaattca ataggaagtg taacggctct
    43741 atctctgtct tttttaaaaa cttccatttc tcttttgaga attgtttttt ctggaggatt
    43801 ggatgcaatg agtgcttcca aattttcaag gtgttgtttt gcttcgtctc ctgtaaacat
    43861 cttgtgcgag agagaagaca gttgtgcaat ttgctctgct ctttcttctc tagcatcatc
    43921 gggcatcatg acttcactat cccaatgcag aagactgatt atatccccta aacgagtgta
    43981 ttctttccaa aaatttctat aggattgtaa ggtttggctc atatagatgg catattttta
    44041 tatccttcta gagaaaagta aatattctat tgacaggata aatctattta aaaatataac
    44101 attatacatc ctgcgggcgt ggtgtaacgg tagcacaata gccttccaag ctcttagagg
    44161 gggttcgagt cccctcgccc gcttcgctgc tcggggtcca ccctttctcc taatggagaa
    44221 actttgcatc ggctcctccc gctgatggct ccgaagcctc acacccctcg cccgactttt
    44281 tattctagaa ttatttttac actctttatt tgaatcactc catcggaatt cttgttttcc
    44341 aaatttcagg attgcgactt gtatgaaaca caccgataat ttcaacttcc ttttcttcca
    44401 aagaaacttt atagaaaaga gcatattgaa atgtattcat taatgctcgt ctaaattctt
    44461 tataaatctg cggatatatg tttggatgat tcgataaaaa ctcaatggtt tcctcgaaag
    44521 agagtaaaaa ttcatctccc aatccagttc gaatttcttc ataatacttt ataatttctt
    44581 taatatcatt ttgagctgga agagaaatga ttatctcata tatgttttcc attcaattcc
    44641 tttttaaact caatccacgt ttttgcttta ttcttatttt ttagatgctc ctcccagcgg
    44701 gcatccaaaa tctgttttcc tttctcagaa agttcaaaat tagaatcagt tgctacatat
    44761 cgaatatata aaaaatcaat atactcttct aaatgatctt gtaaaaaggg aggaagtttt
    44821 ttaatttttt caagggctaa tactggttcc atatttctat tatctgcgat tataaaccct
    44881 ctgtcaagac attcaattta tcattatgat tcctaaatcg gttgttattg gaatctatta
    44941 tcgatacact aaacattcct taaagatagg actaaacttc ccctaaagat atctccggag
    45001 ataagtttag gataagttta agggaacttt actcctaagt ttaagggata tttggtggta
    45061 agagaatgcg aagattacca ccgataggga cgatacgcac cgatggagtt gtttagatag
    45121 atttggatat ttgattctat cggttgtttc attggggata tttggaagat tgaaccacga
    45181 agtgcacgaa gagacacgaa gaggcacatg cggaggcgtt ggtcgcgaat acttggtgcg
    45241 aagggtggac tagacacgga tgaggtttga tttttttttc ttcaccacga aggacacgaa
    45301 gactcacgaa gtgaatgaat aataaatctt ctcttttctt cgtgttctct gggctcttcg
    45361 tggttagatt tttttttaag catttacaca atccctatgc tgtttgggta atatatcttt
    45421 cctttaactc ggttacggtt tcagggatgc acttcttaaa ttttgaaatg gtcggaatgc
    45481 aattatctga gtttcgtatt gactacgcat tatctagtga cttggtatac accaagcaat
    45541 ttttatgttt tcccttacgg actttatttg tttaggtttg gtggaaccga acgcgctttc
    45601 tagaaagtga taagctttat atctgttagc gttcagttta gtggttcgta tagtgttggt
    45661 atagtccttg gtatattgga atgggggctt gagtattgga gggagtatca tggctattaa
    45721 ttcaaatgtt ggttgtatgt atgcttgttg tttttgaatt gataactaga actaaagtat
    45781 aagtgggatt ataacctctc ttggtagaag aatctcttct ttcgaaaaaa agcttcagta
    45841 gttcattttc tcggactaaa acatttagat tgaattgaaa taaagtctca taacgttcct
    45901 caccgattca attcttcttt taatggaaat ctgagtttgc cgatataaaa attctcacaa
    45961 gatcgctgat tggattgact gggaaaggaa ttgcatctat tataatactt atgcaaaagg
    46021 ctaaaatcaa tcgtttccaa tggatagctg ttttcttctt aagtttcttc tttggctgtg
    46081 aacactttct caattttgtt ttaaaggacg aagagctagt gcaagttcca gagcttgtgg
    46141 gacgttggtc ggcatatgag aagggaaaag aaacggctgt tttaaagata gaagattttg
    46201 gaaaaaatca tgagtatctc ctaacagtag aaaaagaaaa caaaggagca cttcttcgaa
    46261 ttactcaaat aaaaaatatc tatgtaggag aatatcatga ttttgatgaa aagaatcaag
    46321 taaaacccgt tggttatttt ttcattctaa aaaaagtaaa tcatgatttt caaattttta
    46381 taggcagtga tatcacaaaa aaaatggtag atcaatataa attagtagca aaagaaaatc
    46441 aaatactcgg aaagaaatac atcacttaca gtttatcggg cacagatcta caaaatagat
    46501 atgctctcga attactttta tctgatccag attttttcca tttaaaaaat tcgtataata
    46561 tgattctaaa aagaaattcc attcaaagac tataaatcca aaccaatctt cctttgcata
    46621 ataaactagt ttgatctata tcgattttca aaatcgatac aattgagaag gaatcaatat
    46681 cgatgcttta ctttcactca ccagactgta ataaaacttt atatttagag atagaatcaa
    46741 atttttagat cttctcttta ttgttaaaat tctatgcagt ccagtgagtt ttcctaaatt
    46801 attttttcaa agggaattgt tcagcaattc tttctgcatt ggcagcgatt gtaagagaag
    46861 gatttggacc tagagaagtt ggaataatac tcccatctac tacaaaaaga cctttatgcc
    46921 caaagacttc tccttttgta tccacaactc ctgtctcagc actctcgccc ataacgcatc
    46981 ctccaattgg atgcactgca atgatcttat gtaaataggt aagtggattg tctaaaaaat
    47041 caccttccat tttttctgcg acgtttttca tggcagatcg aacacgatca taatgcaatt
    47101 gactttcttt catttcccat ttgataattg gttctccgtc ctctctcaat ttaatctctc
    47161 cattatttct gtctctcccc atacccaaga gtagaaaagc agatcgaaca aatttttcac
    47221 tgtctagtaa atcgattagt ttatcaccta tattcacttg tcctttttga agaggagatc
    47281 ctattaactt aaaaaagaat tcctttgcat atcgaatccc tactttcaca gctcgtaaaa
    47341 taaaatgaac agaaggaatt tttccagaaa cataccagcc aagtccaatt ggaaacccag
    47401 catcttgaat gactgcatta tgagtgtatc catcaggata cggtttaaac ttatactgaa
    47461 tagcagccgt tatgacagga ccctttgttg gaataacgtt atgcggagta tttaaaactg
    47521 atccttccaa atctccgttt ccacaccact tcttcccaag cattgtgata agttttttag
    47581 tttaccttct ttctgcattc gaaggagcat tgcagtagaa ccaagagaac cagcagacaa
    47641 tacaacttgt tttgcaatta tctttgtttt cttttctgaa ttttctggat cagaataaat
    47701 gacttcgtat ccagtgtcat ccgccaaagg agaaatagat tctacaagag cgtgagtttt
    47761 aacctctgct gctacttttt gtttcttcgc tctaaaaata taatttagat ctaaagtatt
    47821 ttttgcatga atattacagc caatatcaca ctccccacat tttgtgcaac tagactgaat
    47881 tgctccatgg gaattttctg tctgatgtcc tgcaaactct cctttaaatc gaatcgcaag
    47941 gtctgggaaa ataacctttg gtttttctaa agcgtcagac ggtgtatcta aagattcacc
    48001 tactttttta aaaagatgtg tttttggggt tcgattgtaa taaggatgat caattggata
    48061 tggagaggct tccattgtat cgattacttt ttgataaaat ggttctaatg tagatcgatt
    48121 gattccacca ggccaaccag taaaattctc ttctggcatt ctcattagaa catttgcata
    48181 gattaaacta ccacccccta gaccgcttgc agtcacagac ataagatcgc tctcaggata
    48241 gtctcttact tccatgaatc cataacgtcc atcttttgga tcccaaatta atttttcctt
    48301 tatttcattg attcgtcttg gaaattccca catcccatat tgctttcctc tctctagcaa
    48361 acagacttta tatccctttt cagaaaggcg acaactcata acagatccac caaatccaga
    48421 tccaattact ataacatcat aatcaaaact actagccata actcccccaa ataatttact
    48481 ttcgatgcat cttaattaga aagttcaaat taaatagtaa tttattttcc tgtttttatg
    48541 atatttagca gaaccatttc tggaagggga aaatcactgt tatatgtaaa ttcagtttct
    48601 atcccaagaa ttcttgtgtt tagaaaagac atactcaaga acgattcaaa taatctatct
    48661 aaatagctaa atgaaaaaac aatttgacaa atggataaat agtcttaaat actttttgtg
    48721 gagttctttc ttgaaaacaa atatccgaag acatgggaga gtttgactct accaccactt
    48781 tatctaaacc aagtcagtga gtttggaagt attaatatct tcaatgataa aaatgaatgt
    48841 attgcgagtg gatggtttaa gtttatcatt tctccaaaga ttatagttct cgaaaattta
    48901 ggagaagaaa gaaaagcctt tttagaaaat acaagaatcc tagatttcat ctatcaaatc
    48961 tcctctaaaa ataaattctc tgtttcaaaa agtatttttt taaacaacgg gaaaactctt
    49021 caattagagt ttttagaaga agaacataag aattatactt tactcacact aacaaaacca
    49081 aatccagatt tgtgtaaatt tactatccta aattcttact cgttccctgg tttctggacg
    49141 atagacacag atggtttttg gggtaaatcc ttttctgttt ctctagtcgt atcctctgct
    49201 ggatatgaaa taatcctaga aaatgatctg catatgtgga aacagtgggg cgagattctt
    49261 cctttttatg aaggttccac tgaatggata attactctac taacaagttt aaattataaa
    49321 tttattcaga aagatccttg ccaattccaa ttagaatctt tatctcaatt tttacaacta
    49381 gctgatttat ttacagaaag agataataca aaaaaataat tttctttgta ttatttattt
    49441 taactctctc tgctcccatt atgttcaaaa gagaatagat ctgactcgat tggatttagt
    49501 atttcattca taggaattaa ctctgatgag cttaaaaaat ctttttctga ttttttattt
    49561 ttaattaaaa aatattttat aagtattgct ttaaagtaac tgataagatg atctggatga
    49621 gagttcatat cggagacaca ataatcaaaa tcttttctaa attgttcact ttcttttaaa
    49681 attggttcta ataaatgata gtttttatta acggaggaaa aaaacgcaat ttgtctttcg
    49741 taatcatttt cctttttaaa aaaacaatat gcatgttcta aatcatgtag cacaaattca
    49801 aatgcatctc ttttctcttc aattaaactc ccattaaaag cattttcaaa atcaattgtg
    49861 atatatcgct taccgcatga ttgcaattct aacatctctt tggaatcagg aagataattc
    49921 actaaaacaa aacgatcatt gtaaaaagca tcaaacaata cgtcacgtat tgaattaggc
    49981 aaatatcgaa atgaatagag ccttagaaac tcagagatat aatttgtttc aagatctaga
    50041 gatgggataa aagtaaattc aagcggaaac tctttattga tttgaattcg acttctattg
    50101 ataggatttt tttgaaaagg gtaatccttg actctaaagt attgatatag tataacaaat
    50161 agagaaataa tctcgattga gcttaaagat ttttcaaaat aagatttttc tagaaatttc
    50221 aaatagggtt ttaaaattac tgattcttcc tttaaaaaat cgtttggtct gagtaatttt
    50281 ttaaatctac cgattagttg ctcattttga attggcatga tgaataattc taatctttat
    50341 tctttaaaaa agattgcaaa taaaaaaata cagcaattca atgaatgcga attggagccc
    50401 gattgtcttt tgttaaacga agttctagca attctctatg ttcataatcc ttatccataa
    50461 aataagcttt caagataatc tctaatttct ttggtaagga ttctctttta taacgtttta
    50521 taatcgatag gcgaataggg tttgtatcat tgcagtcttc tatgaaatct tttaaatgag
    50581 aacttggatt tttatttccc aaatccttcc agattgattt atactctaag aaatattcta
    50641 gtttttctgg ctttaaaata tccttatttt caagtaaatc tgcatccata ttttttatac
    50701 gagatagatc attacaagtc agattgattt gagcatgtat gtttgggtta tgataatatg
    50761 ggtatggatc atctgtaacg atagttgctt ccattctaca atccttacaa tacttactag
    50821 gaatattggt aatagtatga atagatcctt tagaattcaa atattcatac agattggaat
    50881 tacaactaga tataaagaat aagattaata cgatttgaat cagcatttta ttttctccaa
    50941 gataagtatt ggattcaact gaacgaagca tttctttaaa agaaaggatg atcttttcta
    51001 aatgggtgtt gttatatttt gaatagagat aaatcttcaa attaaatgtt tagtttcatt
    51061 tgtattattc aatcttctca taatgacctt cacagttaca attcactcct ctgtaaaaag
    51121 ctttattttt ctcgatcttg tagatttgtt caattgcttg aaactccaat actctcttta
    51181 aatcgtcctc tgttagattt ggatctttgt attctaactt cactagttta tacatacaat
    51241 tttctagaac tataatttta gatttcattt tatattccga tttcccctcc ggaaaaaaat
    51301 aactaaacgt tccatctctc accacaatat tcttttcgcc taactctcct ggctcaataa
    51361 acttcccttt gtaaaaaggt gtgcaatcta aaccagactt agttccttga ttttttgaat
    51421 cttttttccg attcgaaatt ttagcagaaa ctggactcat gagaattgtt agaaaaaaac
    51481 aaaacaaaat aaacattttt actttcattg aatcaatcct ttgtttaatg aaaaatgaaa
    51541 tcaacctgtt ttcaagttcg atttaataat cctattgaat gttttatcta atcaattgaa
    51601 aaccattttt aataggaaaa attgataaaa tatcagaaaa gagttcccat tcaattcttt
    51661 gattggattt tttccaaaaa tcctatcgac aataaaacca atcaaatcaa ttctcataag
    51721 aaggtatggg tgaaactctc ctttcctact gattcagagc gcttactaaa aattaaacct
    51781 tgcttttatt caaaaaagaa aacagtgcaa agataggatg agttaaaaat ttatacttat
    51841 aataattctt aattttgtat gtataaaaat cacaaaagtt tatacaccat aaaaacttgc
    51901 ttgaattaat ctatttaata ttcttcttat ttttatatga aatacctagt cataatacta
    51961 ttgctatgct ttaacgtttt aaatgggcag gaaactatcg acaatcccgc aacgaaggta
    52021 gaaaataaaa agcagtcaga agaagaaaag ttttttagcc ttgaagaaaa tatttgggta
    52081 agtgtcgctt ccaaaaaacc gaaatcattg cgtgaaacac atggaattgt aaatgtttat
    52141 acaagtaaac aaatagaaga ttatggattt ttaaatttat ctgatacgtt aaatacaatc
    52201 ccgggaatga gaagtggata tgcgttaacc caaatgccta ataaccttag gtctagagga
    52261 atcgcaaatg caactctcat tttggtcgat gggcttccaa tgattcaaaa aaatgaagct
    52321 tatggtttaa ataatattcc aatcacaata ggagctgttg ataaactaga agtaataaga
    52381 ggacctggaa gtgttctttg gggagctaac gcatttatgg gaatagtaaa cattgttact
    52441 aaagacgcag acgaattcat tcaggatgga aaaaagtcgg gttacaaagt tacaatgcga
    52501 actggcaatt ggaatacaaa taatttctat ttcaattcag gtgcaaagct atcagaaaat
    52561 ttaaatattt acgtcggagg ctcttacgaa agcacgctgg gatatgtgca aaaaacaaat
    52621 agcactttta caccaaatgg attttctgtt tttcaacagc caggaaatga tattctagct
    52681 caatccaatc aagatgcaat caatagcgga aacacaactc ctgctgattt aaaaattccc
    52741 tgggatagag gaacggaagg aacaaaaaat cgacgcaacc tagattctct cgccgagttt
    52801 atggttaaag caaattggaa accttacgag ggtggaaatc taaaaatatc tcttatcaca
    52861 aatagaaata aattttattt tccaattagt ggagctctca atcaatcaga tagaaataat
    52921 aacggttatt acgagtatcc ttataatatc tattccatta aatactctca tacttttagt
    52981 gatacactaa acacaacttt gacaattgga aaatatgaat taacagaatg ggagcatttc
    53041 cttcgagcag gtgcttatta ttcctcccct agtttttcaa atcagaactc actcagtggt
    53101 tcctcctctc gtagaaattt ggatccagat gtaacatctc aaggaaatta tttggaaaaa
    53161 ttatcaaatt tgacaatcga ttggaacgtt agctatacag gaataaaaaa caatactata
    53221 ttaatcggag ccacttactt agagcaagga attccaaagt tatattttag tcgaagcaca
    53281 agtctaaata actcaaatca aatcactcaa gacagacttc ccgaatggta tgaccaagga
    53341 cctgttaaat caaaaatcca atctttgtat gtccaggata gtataagaat acatgaaaaa
    53401 ttaaacctac aggttggtgc aagatacgat aagcactatt taagtagcag ttttatcaat
    53461 ccacaatcta gtttaatctg gaatataact tcccgcctaa caggtagaac cctctatagt
    53521 cgtggattta gagaaccttc ctataataac ttattttcat acaatggcac tgtgggttct
    53581 attacctatg caggaattga ccctgaccca aaagacaaag atcccagagc agattctcct
    53641 aaaaaattaa atccagaaaa aagtgaatcc tatgaaagtc aaatttccta ttatctactg
    53701 agtgattgga agttaaatat aaatctagct agatcgaata tcagagattt gattacacct
    53761 tattattcaa attctcaatg gacatggtat tttaataatg ggagacagca aatcgattct
    53821 ttggaaggag agatttccta taacttatca aaaaaacttc tttttatgga gaaagggagt
    53881 gtattttttt acaatgttac ttataataaa attacaaatt tcgttacgga aacagagtct
    53941 agagattata cagcgtctat cgtaaacagc aacaatccac caagtgaatg ggctaaaaac
    54001 gattttaaac tttatacaca ggatcagaga ttaattgtta aaacagatcc aagatactgg
    54061 atgaatattg gaggcgttat acgatttctc gaaaactatg catttaattt taccgctttt
    54121 tattctgcag aacgttactt tggaggatac aaaggattaa actctggaac aagattttat
    54181 ccagaaactg acaaagatta tttaaatata attgctgcat gtggctctaa tcaaacatct
    54241 tcttgtccag caatcgtaaa tgtaaataca agaaagaatg gagttcgaat tggttcttac
    54301 tatactctaa acattggttt gagttacgat aagattatgg agaatggaag taaaatcaga
    54361 atttctctgc aaatcaacaa tctatttgat agaatttata atgctcccta tacaaatttt
    54421 agttcaacag gcgctcagat tcttccaaac ccacaacccg ggcggcaaat ctatcttacc
    54481 ctcactggat atttctaaga atccaatgtg agggaaaaat aaacatttgt cgggaaatta
    54541 aaaaattgca ccaagaccta aataagcggt tcgcccatct gccggaagaa ttccaggtcc
    54601 aggatatcca ctcgctcttc tggttgcata aattgtattt tcaatattat taacgccacc
    54661 gcgcaagctt agaatctctg taatattcca agtaaatgat aaatcagaca ctttataact
    54721 acttaatttt ccaatttgac tattgggagt tagagacgtt agagcagatc ctgttgtgtt
    54781 tagattcgct tgaagaggat gctctgtatt atttgcatct gtgtaaacag aagcaacttg
    54841 ggaattttga atggtaaccg agaagagtcc tttttttgta tatgtgagtc caatccgagt
    54901 aatcctatcc ggtgcattct ccacacggtt tccaacaatt cctaagtctt ttaaatttcc
    54961 attgtctggt aaaagaattc ttccaaagtc tgcactttga attacaatag gatttgtaga
    55021 acgaatgtat tctgctttga cttgtgcata ggaaataaag cccgaaatgc ttccccaaga
    55081 tgtcttttca aaaaaatgag tgatcggatc atattctaaa tacgcctcta ttccacgact
    55141 tagactgtct cctgtattcg ttctaaaatt tggacgcgga ttttgcaaag cggcaattaa
    55201 atcagttcga ttatttgcac tgattgtccc tacacgatta ttgtatctaa gctgaaatgc
    55261 accaacatcg aaagttagaa aattggaaac agctccacga tagccggcat ctgcgttgta
    55321 tccattctga ttttttaagt ttggatcgaa gtaatctaca gaatttcctg gagtaaataa
    55381 ctcttggtaa agaacaggtc taaaaccctg tgtatagttc gcatagatat ttgtctcttt
    55441 tgtaatttta tattgaaatc ctatacctgc caagagaact ttgtttcgaa ttctctgaga
    55501 gttcgccatc acataaggag ttgttctaga atcaatagaa ttcggtgtag aactaacgga
    55561 tagattgtct gtgctcaaat gtcctgaaac ttcactttga atccattcat atctcacacc
    55621 gggcgttatg ctcaaagcag atgtaatttg aagtaaatgc tcatagaatg cagcataatt
    55681 cattgttcta aaattcaatt ctgaatttcg actcacaaaa tttccaattc gattggtttc
    55741 cgaaagggta aagtcatttc ctcttgttcc atttgtattt cgaactcgaa gagtatttcc
    55801 aataaagtat cgtaatccaa acgatgaaga ttgattcact ccaaatagag tataggattt
    55861 taaaaagcga aactcacttc catagttgcg ataccaatct cgatcgactc tacgcggaga
    55921 aaatgcatct tccatttcaa atccagttgt tcttcctgtt gtcaatcgag aagtatccgg
    55981 aacgttaatc gcagccacat tcccaacaga atttttctcg ccgtctaatc caaagatttt
    56041 aaaggatgct tttgtttttt catcctgttc aaaatcgaca gtaaacacgg gtagattcca
    56101 aggagcagaa agccaatttc gagaacggtt ggattgtctt ggattcactt ccgtagtcgc
    56161 agatgtcatg aggggatcat atttcatttg accgtcttgc aaaccacccg cttgttggct
    56221 ggtaaaaaga gatttcgtaa attctatccc aaattttaat ttttcagtcg cttggaagct
    56281 tagatttaca aatcctgttt gggttttata ttcagaatcc ttcctccatc cgtcggaaga
    56341 ccgatggtga aaaaatacaa agtagctgaa ttttcctgtc gttcctccga cagaattgta
    56401 ggaattgaaa agattgtagg atccacccgt ttgtcggaac tccacagcaa aaggacgacg
    56461 agggtctgcc tttttcattc tataatttaa caaacctcca aattggggac cgtattgaag
    56521 ggcacccgca ccacgaatga gttcaatttt ctcaattgcc tcaaggggag gagtgtaata
    56581 agcttctgga tacccaaaga catcggaggc aatgtcatag ccattttgcc tgacattaaa
    56641 ctcccaagat cgattggggc ttaatcccct agttgaaatt gcagtctgaa ttcccgaccc
    56701 gtcattttcc caaacggaaa cacccggtat ttttccataa acttgtcgat tgttgttggt
    56761 tgtaagattt gcatttaact tggttagatc taaaacctca tttttcttcc cagataaaat
    56821 ggaagttcct tcaatttctt tcatatgtgt gattccagaa aaagatcttt ctcctttcac
    56881 ttgaatgctg gattcactgt tttttggttg ttggctttct tcctttttcg atgactcctg
    56941 agaatgcaat tcgaataaaa gaaataaaag actaaaaata aatacgagga tttgcatgaa
    57001 acacctttaa tgatattaat tcttattttc aattacataa atttggaatg gagtcagaag
    57061 taaacctaaa attctattaa gcgaccagct tggcgagtta gggataaaac aaatcctatc
    57121 taagccaata aattgccctt atttaaaaga ttttcaagtt acaagactcc aaactccctt
    57181 gaaagagaga ttatagaaaa aaatcttgat tgcgaattcc tttgtgaggt tcgaaaagat
    57241 caaaagagga ttggataaaa aagagggaat agaagtcttc cgatagaaat gtttttaaaa
    57301 aaatatttga tttcttttta tcttaaataa atgtataagc tttttaaaca tgaatctccc
    57361 acaccaataa tttcagtttt atgtctaaag ttataggaac taagctatgt ttaaactact
    57421 attctacttt ctttttctct ttatcttatc gtgtaaaaat gatttgaacc aacaaaatac
    57481 tgatttggga tttttaagtt tcttaattcg acttagtatg ggagctgtaa atacctccaa
    57541 tccaataata aatccagaaa atgattttta tgccaatgat agtttagctc ctgtgttaat
    57601 tgcaagggat agagaattgg gtgtaaaatt tggaacagaa atttctagct gtgatttaga
    57661 atttaactct acaaagataa ctccaatcgt gcgatatgcg tcagatactc aaacaaatta
    57721 tttttcacca cagccaggat catactggcc tatagatgta gtaaatcctt ttactttaaa
    57781 gtttagtaat tgtatcagta agacaggttc tacttttgat acaagcgcaa tcagtatgaa
    57841 gttatatgta gcagaaaaag taatttacat agatggaatt ggaggagttg atacaaattc
    57901 tggaacaaat actctaccag taaaaacgat accagcaggt attagtctcg ccagtgcttc
    57961 ctgttctgac cgatgtgcga ttgcactaaa gggcggtgaa tacatactaa actctggaat
    58021 tatcgttcct actaatctga gtatatttgg aggatttgac ccaatcgatt ggcgaaaacg
    58081 gcgagcagat aaaactgccc tacctcctta tgatacgatc attaccgata actcatctgg
    58141 tattacaagc acaggatcgg atccttactc gactcttaaa tttttaaatt actctggatt
    58201 acaaagtaaa acagtaatcg atggtatcat tgtaaatggt ccaacaacag caaattcaaa
    58261 tagctatatt tctcctatcg gttctgttgc actaacttct ggtagtggtt ttattataag
    58321 aaatacaatt tcaaatgata gatccacatc cgctttatct atcacttcgg ctggattctc
    58381 ctcaataacc agctcaggat cgatactgat tcaaaactca actctacaag catcaccgat
    58441 tgtcgctgca acttctacaa gatacggaat tgtttacgca agttctgtga ccggtagtag
    58501 ctttacgttg gaaacatcaa tagtcaatgg aggagtgtca tccgttggta gtagtggttt
    58561 ttttgcgaca ggaagccctt ctggaaatat acttctatca aaaaatacga tcgttagtcc
    58621 cgattgtagc acaatttgtg ctactgtggg agttacaatg gattttacaa ctgcttccgg
    58681 tgcaatcatt tcagacaata caattcaggt caccaatgga aataattcta gaggaatctc
    58741 aagtagtatg ggatctggaa ttcaaattaa aaacaatatg attcaagtta cagggataaa
    58801 ctctggatct gtatttggaa tctcatttgg tggaacttcc tcaaatcaaa tcgttgatgg
    58861 aaatacaatt acaacctcta cagcgggtgc tggtggggta aataaaggaa ttgatatcaa
    58921 tggcagtgtt tcctcgatca atgtattaaa taatacaatt aatactggca tttgccctgc
    58981 tgcatcttgt gctagaggaa tacacaagca aaatgcatca aattctacta ttgttggaaa
    59041 caatataacg ataggcaatt gctctaacgc ttgtgatgct cgagtcattg actctgctgg
    59101 cggtggaagc aatacaatac aaaataatat tctaagctat ggagcggtaa catccggaaa
    59161 tcccgccacc ggtatagagc tcaattctgg aaactttacc attgatggaa atacgatcaa
    59221 tggaggaact tgcacaggca gcgtgaactg cactggaatt aatatttcag ctgctacaaa
    59281 tataacaatt acgaataata caattacagc gggaagcaca acaggcactg ggaatcaaat
    59341 tggtgttctc atgggtgctg ctgcaactag tttcactttt acaggaaata ctataagctc
    59401 aggagtcaat agtggaagtc ctgccaatcg aattgctctt gatcttgtaa attggcctaa
    59461 caattccacc attcatagaa atacatttac aaatctaagt ggaagtggaa atgcaattgg
    59521 agtcaggttt tctactctag caaattcctt gcgtttttgc agtaatctaa ttttggctgg
    59581 aaatactacg ttaggggaat cggtaggaat taacgcacag agattaaacg gaggtgcaaa
    59641 atttttagga aatacaatcg tattaggaac ggcgagtgtt tccgcttctg gaatgaaatt
    59701 tgtgacaaac tctagctatg ataatatgga aatcctctac aatattgtgc aaggaaatgt
    59761 agcacaaact gcctccactt cttgctttgt agaacaagga gcgataacct atatcacaca
    59821 tcagaataat tcagtctcta attgtagtac ctattacaac gaaaatggaa tcgtgcgctc
    59881 tctattttgt tcaggaaatt ttggaaatgg tggttgcgga gctcctcctc aaactatgca
    59941 atttccaact gctactggaa atacaatttc aattcccgtt tttgtgaatc ctgcctccaa
    60001 cgattataga ttaaatacat cttcaacacc tgtagatatt ctaacaggga tgaataatac
    60061 agctacctat actagtctgt gtggagatat tttagataga gacggactcg ctagaggact
    60121 caatacatct ataggtgcct atcgttagta tcattttaga tctaatccga gattttttat
    60181 ttatgagcta aatagaattt ttttttactc attgtatgtt ttttataata aagataaaaa
    60241 gttatttata caccaaagga ttccaaatat tttggtaaat tatttaaggg atatatgttc
    60301 acatctgaaa tcaaaaatat tacaggaatc catacagaga ttttaaccta tcgctcgact
    60361 tctaatatac aaattttaat ccttccaggt aatccaggaa tcggctcttt atatatagat
    60421 ttaggaaaaa tcttatacga aaaattcaat gggcagctaa atctttatct aatcagctat
    60481 gctggcttta ccaaaaaaaa atctgaaaag aaatataccc taaaagaaga actagatcat
    60541 aaaataaatc tgattcaaga attaaagaaa gaatggcatc ccgactctaa agttctaatt
    60601 gtaggccata gcataggtgc ttggctcgca aaggaaatat ataaggaatt aggaaataca
    60661 atgaatatat ctctttatat gttatttcca tttcttgcca agtctgacaa tccaatgcaa
    60721 gttaattttt ctaaatttct tgcgaatcca aatcatactg ggatgtcaat taacgcttat
    60781 aaacttatca ctaagcttcc cgcttctatt ttcctttctc tagcaaaact tttatactcg
    60841 cacgcatcag aaaatacatt ccaattgatc actgattatt ttctattaga aaaccatatc
    60901 attgaaaaca ttttttatct agcaaacaac gaattcgaaa ctttaacaga agactttgat
    60961 ctggaatttt ttcaagccta tccaaaaaat atcaatctat actattgccc agacgacatg
    61021 tgggctcctc tctcacaaat agaaacaatc aaacaaaata caaaagaagt aaatgcggaa
    61081 atactcaata cacatacgca tgacttttgt gtaagtttat atcaatgtga aatcatcgca
    61141 gaaaaaatta gaactagttt aatttaaaat gaagaaataa aaaaagtatt cattttgcaa
    61201 ttaatgctca ttctctcaaa ataagaaaca aatcaattga attcactctc taatgtttca
    61261 accagcataa attagatcga tagacgataa gaaaaacaac aaacctatca ctgaaaggaa
    61321 agtttattac gactaatatt ttatttatta gaaggattga tctataattc tcattttttt
    61381 gtagacttaa ttttacatta attctattct attgcataaa ggtaaatatg aagcaatcac
    61441 cgttattatt aatagggatc gtcgtatccg cagatgggct ttcaggattg gaagacttct
    61501 ttaaggcttt acgcgatggg agtgattgtt cctttattat aattcaacaa caaacggatt
    61561 caatagagaa taacttacat aaactcttag ttagttgcac aaatttacca atttacaaaa
    61621 ttgaggatgg attgcatcta gaagcaaatt caatctatat tgccccaccc gatcagttgg
    61681 tctcactcac tgaaaaaaga tttcatttag aagccagccc tgcctcttcg tttgctccaa
    61741 ttaatttttt ttttgaatcc attgtggatt ggagagaaag agcagtcgga gtaattctat
    61801 ccttagattt tgaggatgga gcagaaggaa tacaagccat cagtcaagtc ggtgggctaa
    61861 cgttagcagt aactccacat acctctcaat ttgcaacgat acaagagata gcttctaata
    61921 tagatttttg tgattataaa agcacgcctt ctaaaatgcc tgaattaatt ctggattatg
    61981 caaaagaatt tagcaacaag gattctttgg atatttcaga aactctcaaa acaaatattt
    62041 ttacattgat aaaaaatacg cacggaattg atttcaacca atataaagta ggcacaatga
    62101 ataggagact gaaacgccga atagaattgg caaacctaga ctcacctgat tcttattatg
    62161 cttttttaca aaaagaatct tcagagattg atcttctagt aaatgacctt ttaattggga
    62221 tgacagattt ttttagagac ccagaagttt ttaattattt agaaactgaa attttaccta
    62281 gtttatttag aaacgcaaaa gaggatggga ttcgaatttg ggttaccgct tgtgctatgg
    62341 gacaagaagc atatacctat gcaattctac tctatcaaat tgcacaaaaa ataaattatc
    62401 gtggcaagat cacgatcttt gcaaccgacg tcaacaaacc gtcattagct atagcatcct
    62461 taggtattta cgaacccaca cagctatcaa acctttctaa tgagcaaaaa gaaaattatt
    62521 tcttctatga tataaataca gatctttatc gaataaagcc agaaatcaga caactcatca
    62581 cttttgcccc acatgatctc ttgcttgatc ccccttttac acgcatggat tttgtaagct
    62641 gtcgaaatgt tctaatttat ctaaagccag atgcgcatga acatatttta tcactcctca
    62701 actttgcact taaaatgaat ggttttttat gcctaggcgg gagtgaaggt cttggaagat
    62761 tgagttctac ctttgcgtct gaacattcta attacagagt ttatcgtaaa acgaaagaat
    62821 ctagattctc tcctgaattt agaaacacta gatctttttc tataccttcg atttatcctt
    62881 atgattggaa tcaatcctcg aatcagacaa caacgattga tagagatctt ttacaagatt
    62941 atgatttatt gctcaaccgc tttattccac caagtgtaat cttaaatgat caaggacaag
    63001 tattacaata cattggaaac gtttctcggt ttttaaaatt accatcggga cgaacagaaa
    63061 ataatatttt agaaatggtg gacggtgata tttacattgc tgttagtctc ttactgagtc
    63121 gagcacggaa caaagagcca aaccttaagg cgattcttgt taaattgcca gatggggata
    63181 gagtggaagt aagagttgaa gttttaattc acaaaaataa tccgagtatc cattatcatt
    63241 tatttttttc tactgaaaag tttccatgga acgaatctac agttttgccc ttaaaaacga
    63301 atcctgaact tcaaagacgt ttattcgaat tggaaacaga attgcaagtg ggaattgtaa
    63361 aagagcgagc tttagtagaa caggcacaaa tcacgaatga ggaacttcta gcaacaaacg
    63421 aagaattaca gagtgccaat gaagaattgc gatcagtaaa tgaagaactc catacggtaa
    63481 attctgaatt tgaaaatcaa aactttgcct tgaatcaatt gaatttggat catgaacaat
    63541 tactcaaatc gcttcaggtt ggtgtattat ttttagatga atcgcaaaga attcgcaaat
    63601 ttaattctgc agctgctgaa atttttcagt tattgcccca agacattggt agacctctag
    63661 cacatttttc tttccaatta gaaggctctg gtaatgttcc tatggatgca atagacgcaa
    63721 tgaaatctgg aattccagta gaaagagagg ttcaatctag agacggaaag tggtggctta
    63781 aacgagctgc tccttatttc cattcttccg gacaaattcg cgggaccgtt gtatcttata
    63841 cagatattac aaacataaaa aatacagagg aagcgttaag aagaagtgaa gccaaattcc
    63901 aagcagcgat taagcattct tccctcattt ttgcacagac agataaagat ttgcgctaca
    63961 cctggattta taatcctaac cctgacttta attccgaatc gatgattggt aagtcagata
    64021 ttgatatatc ggataacgtt ggaactcgtc aacttttaca attaaaaaaa gaagtattag
    64081 aaactggaaa gggaatacaa cgtgaaatca gatttccttt tctaaatgga taccaaactt
    64141 atacaataat agcagaacct cttttagata cttctggtca aataacgggc gtagcaacct
    64201 gctctcttga tataaccgac cgcaagcaat cggaggatgc attgctagaa agtgaagcaa
    64261 aatatcgcaa tctagtaaac aatctgaatg atggcgttgt agctcattca gcagacacat
    64321 ccattcaggt ctgcaatgaa gccgcttgca caattcttgg actaacgatg gatcaaatgc
    64381 taggtaaagt agcaatagac ccgacttgga aattcgtctc tgaacaaggc actcctatgg
    64441 atttaagcga atatccggta aatcaagttt tggcaaaaag aaagccaatt caaaatttca
    64501 tactcggagt atatcgccca atcaattcag attttgcatg gttactttgc aatggctttc
    64561 ctgtctttaa aaataatgga gagatagacc aagtgattat aaattttatc gatatcactg
    64621 aaagaaagca agcggaagat gcgttacgcg aaagtgaaaa tagatttcga gttctttttg
    64681 agaattcccc cattggaatg gctatgattg attcgaatac ggggcaattt atcacaagta
    64741 attctcatta ttatgaaatg ctaggttaca catcagaaga atttcaaaac ttatccttca
    64801 aagatttaac tcatatggaa gatttacaaa tggatctaga caatatggag agattgaaga
    64861 gaggagaaat tcaagagttt acaatggaga agcgttatta tcataagaat ggttcattga
    64921 tatgggtaac tcttaccgtt gttccccttt ggcgcatggg agatacacct aaatatcata
    64981 ttgcaactgt aaataacata accgaaaaga aagcacgaga aggtttgctg caattcatag
    65041 ctcgccgcgg ttggcattca acaaaggaaa atttcctgga ttcaattgcc caataccttg
    65101 gagaaatttt aaacattgat tatgttttga ttgataggtt aaaagacgag atgaccgctg
    65161 aaacagtagc cctctatgcg aaaggtagga ttgttccaaa tatacaatac tctctactgg
    65221 gaacaccatg tgaaaatgta attggaaaag aattctgtca ctacagatcc gaaattcaaa
    65281 gactttttcc aaaagactcc cttttaaagg acatgaaggc agaaagttat ttaggggttc
    65341 cactctggga ttcggatgga aatccaattg ggttgatcgc agtcttagat agcaaaccta
    65401 tgaatgattt tcaattagcc caaacgttac tcgaattggt tgccgttcga gtatctggag
    65461 agattcaaag aatggaatat ctggatgtgt taaaaaaaag cgaagaacgc cagcgtttgg
    65521 tagcagacac aactctagat gcttattatg attgggatat gatcacaaat caagtttggc
    65581 gaaaccaagg ctataaaaat ttatacggag agccaacagg aaataatttg atttggtgga
    65641 aaacacatat tcattcggat gattatgaac gtctttttaa atcgatggca aatgcatttt
    65701 cagaacaaaa aagcacatgg tctagcgaat atcgtttttt acgtaactca ggagattttg
    65761 catacgttct tgatcgagga aggatttttt ataatgaaaa aggtaaacca attcgtttca
    65821 ttggtgccat tattgactta acagaacgtc ttcaatttga agatgaatta aaaaaagcaa
    65881 aatggagtgc agaaaaagca aataacgcaa aaagtgaatt tttagcaaat atatcacatg
    65941 aacttcgaac ccctctaaat gggattctgg gttttgctca aacattgaag ctgatagcag
    66001 agcatgtctc accggtaatt ctagatggtc ttaggattat tgaaaatagt ggaaatcatt
    66061 tactaaggtt aattaacgat attttagatt tatctaaaat tgaagctagc aaaatagaac
    66121 ttagtttaaa cccagtaatg ataactgatc ttttagaaaa aataattgat attgttaaac
    66181 aaaatgccat agaaaaggga ttagatttaa aactcgatat agcaactgat ttaccgacta
    66241 tcgttataac ggacgaaaaa tatctgatac aaatattact aaacttactt ggaaatgcga
    66301 ttaaatttac tgaaaaagga gagattacac taaaagtatc tcgtaatggt gaaaaaattc
    66361 tatttgcttt gattgataca ggaattggaa ttccacaaga taaattagaa gaagtattca
    66421 atccttttca gcaactaaac aattctagca aatttgaagg cactggatta ggattatcta
    66481 tctctcaacg tttagtgcaa tcaatgggag gaaaaattca agtagaaagt aaaccaggaa
    66541 gcgggacaaa gttttggttt gaatttgatt taccaaatga gaaagcgaat ctagaaaaat
    66601 ttgaactttc tgaggatcta attaatcagt atgatggaac aaataagcgc atactaatta
    66661 tagatgatga tgaaataaac cgaatggtga tgcgtgcaat gttattaccg ttaggatttg
    66721 aggtaataga agctgaagat ggaaaaatag ggatagaaaa agtccaagtt tttaaaccag
    66781 acttaatttt tatggattat cgaatgcctg gtcaaaatgg attagaaacc acaattctaa
    66841 tcagacaaat gattttagag atacaaccca aaataattat gtgctcagct actgcattaa
    66901 aagagctgag agaaaaaagc ttggaaatgg gctgcaatga ttgcattact aaacctatca
    66961 aattaaaaga tgttttaaat tgcctacaat ctaacttaaa tcaatgattc atttactcca
    67021 ttgattctag cttttgtctt ctcagaaaaa aaagaattta ataaaaatta tttttttaaa
    67081 ttagttcagc cttggtaaca gatctttcca aggagaaaga aaaagcgata gtaggaaggt
    67141 ctctcttctc tcctcattca catcaatcta gtcgatttaa tttttttttg taacaaagag
    67201 ctaaagctag attcatccaa agtcaatgaa atgcttttgt taagtaatct atcgattctt
    67261 ttctactcgt agaaacattt gggttccaga atcatcaaat cctacttcag aagtgaaacc
    67321 ttttggaatg tcttctagta attcaaacat ggcttcctcg tcccgataat ataaaaccca
    67381 gtccatgata tactccatat atttgcgagt ttcgttttca acatggtaat tccctagaat
    67441 taaaattcct ccagggcaaa gcatggagta aagtttttca gtcaaaacct ttgcgacaga
    67501 atcagtaaga taatcataaa gacccataga gtaaataaaa tcatactgac caaacttcaa
    67561 ctcaggactt cctgttttta aaatagttcg aacagattct ttaataaatt caacttggaa
    67621 gggcttttct gttggaatag aatcgattcc agatttcgcc tcacctaagg cttcttcatc
    67681 ttgatccaaa aggtatacct ttgcttggtg cttataagga ctctctctca aaaaatcttg
    67741 catctcccat gcaggtccac aggcaataga tagaatttta aaatcatctt taccagacgt
    67801 agaaagtcgc tgtgacatgt atttattaat aagtcttctt ctatttcgaa ctgcatcagc
    67861 cgcttttgta tcgcatgggt gtttatgaaa aatttttccg aaagaagact tgccaaggta
    67921 ttcatttcga taaagcattt ccatcattgc agaatctcct gcataccctc taggctttaa
    67981 attcgtgcgc ttaatgaatt cagattctaa aatataattc cagatcgatt ttcgtaaata
    68041 aaatccatat ttttcatttt gcaatttcgt aaattctttg gtaagaactt tcagttcttc
    68101 aatcttagca gttaaaaatt catagaattc gccgccgaca ccctttaaga cagattgaaa
    68161 aagagcttct ttcaaacgat ttggttcttt cgcaaattta tcatccaatt catcgagtgc
    68221 aattttataa agggaaagac catatacaaa ctttgaacaa aaaagaataa agttaggctc
    68281 caagtcttct tggatagtaa attgcatacg gattttttta acttcgtctt cttgtaaaaa
    68341 taaagattgt ttatccatga gagtatccat atcgataaac tctctttcga gaaaaacacc
    68401 tagaaaaatt tcatcattca ttgaatctag gtgaactaca gttccagtgc ctaactcttt
    68461 cttatctcca taggcaaata tgatacaatt ttcgattggc tgtgtctttt ttataaaagc
    68521 agactctgac tttggaatac atatacctaa tccaaaacga gagtaatcgt aaatttttcc
    68581 ttttaactca cgtccattta tcagtaatag tatataggat ttttctacat catgcaaacg
    68641 gattcgaatg gtatttcgtc tatctttctt tttcatgagg cgctcatacc tttttcaata
    68701 aaaccaaaga aatatcatca tcctgagact tggctgtaga aagagcttct tccacaattt
    68761 tttgaatgat ttctctaccc gaaagctgat tatactttaa taaactctct ttcaatctct
    68821 cttgtccaaa catctcttta ccttgataat ccaattcagt gattccatct gtaaataata
    68881 atgccaaatc acctgattcc aatttaagag tatgattttg cataaatact tctacatcat
    68941 ctaacattcc aacccaagtt ccataagtag gaaatgtttc aattgttttg gttttatttc
    69001 gatagacaat gatatcttga tgagaacctg cgtaggtcat tccttcattc gatagagaga
    69061 ttgcggttat cgtcatatat ctctccactt ttaagagtgc aatattttct ttaattactt
    69121 tattcaattc tactagaact tggcttggtt tttcttctgc atttttggaa atgattgtgc
    69181 gaacactggt ttgagccatc atcataatca gtccagactc gactccatgc ccagaaacat
    69241 ctccaatcat aacccaagga ttcttatttg tgcgaataat atcgtagtag tctcctccaa
    69301 cctctgtagc gggaagcatg atggctgaga tttcataacc acctagttta tcatcaggag
    69361 gagtaagaga agtttgaatt cgcttagcaa cttccatttc actccaaagt gcatctcgtg
    69421 cttcttttaa gcttttccta ctttcaaatt ctttttgaat taagacatat ttcacatgat
    69481 tgatactcgc agcaatgact acagtagaga gcatgaaata aagattattt accaaattat
    69541 aaatatagaa atcttttccc cagattgcat tgaagattac ataaaataca acggtaataa
    69601 aaccattcaa agctgagtga atagctcgcc aaggtaaaag taaattaact gcaatgataa
    69661 ctaaattcaa tccggcataa taagatgaat tgaatccacc caaatctaca gtcataagaa
    69721 taattgctaa actgacaaaa aaagtaaaag catatccatg taataatgaa aatttagatg
    69781 gtttagtttt acgaattaaa acatattgca ggattacaat ggcagtcgcg gttactctgt
    69841 aggcagcaaa tttaggtaaa agatattttg cttctgaagg aataataaaa atatctaaaa
    69901 tgaagaaaat aggaacaagt gtaaagccaa gaatggttaa aatcttcaac caagaatgaa
    69961 gtataaaatc gagataatca gaaaaactat ctacaaagtc aaaaggttct ggtgctcgag
    70021 gtttgttact aatcgaagtc atctgaatta aattccaact acttcaatta aacctggaat
    70081 attaaaaact cgcagagcgg cgaaacttaa ctcttgccat tttaatcctt ttttgtaatg
    70141 aatggttacc tttctatttt ccttttttgc tctttccagt tgtttaacga taggtgtaac
    70201 cgtagatgag ttcatatatt caaattgagt aaaattcaga atgagtggtt tcgtttgatt
    70261 ctttgcataa attgcatcaa aaataggtgt taaaaacttt gtaggctctc tatctttact
    70321 ttcaccaaac cagttgatta gaatatctgc ttctccatat ctcacttcaa tttttaaacg
    70381 attttcttca tacgttttta cttcactcat ttgcttcctc tgctcttagt attgatagac
    70441 cgcagaaacg gccagtaaat tatcttcatt gacataaaaa tctagaatcg attgtccctc
    70501 ataagcaatt cgaaccaacc ctagaccact ctcaccttct gctaaatttt tattagaaat
    70561 ttccttcaat ctttccacat aggcctcaaa agggttctga taccctcgaa tccattgtat
    70621 ttttgcatct aatcggcgta agtgaactaa ctcagaatcc ttcacctgat tttttacttc
    70681 catcacaata cgtttttttt tcttacgaag tattaattct agtgtagttt ccggattggg
    70741 gtaataccca tatttaattc cattttcaac tagttctgta gtaaccattt tcacagcatc
    70801 tagatcgtcc ttagtcaaat cagagtttgc aaagaaagtt tgaatggaat cggagactct
    70861 atctaattca gaccagtctg ctttgagttg aagtatgagt aaattagatg aatcgttgga
    70921 catatttcat cagtcaacct aaccctaaaa ataaaagtgt caagatgttt ttaataggtg
    70981 caaaagtaga gatactcaat tagaatgaat tgcttttttc aagagaagat tacgaaacta
    71041 gagtccttct gactgaaact catttatgtt gccaattgta aggcattgca ggatcgaaat
    71101 ctttgtctaa atagcatgac ctcattctat aaattaataa agcaatttag accaaagcaa
    71161 gatgatttaa aaaacttaaa ctggaattgg attctctatg accaactctc gaataaacat
    71221 aaactaattc aaaatactgt ccaagatgga cttatcttta ttgaatcaaa agcaaaaccg
    71281 gagtgtagaa attatcataa acaaaaactt gtttatgtta tatcctctat gcgtaatttt
    71341 gcgatcgaaa aaggaaaaga aggatttaaa atactctatt tctattcgga aaaggattat
    71401 agcttagcat tagaagaatt aaaaaaaaaa tatacactca atcaaatcaa atgcttagaa
    71461 ccctccgaat atgaagtcta tcgttctata caaaagctta cttttttaaa gattttagat
    71521 aacgatttat acatttccaa tttagaagaa ttccaaagca tatttaaaaa ccaaaagaat
    71581 tttaaactag aaacgtttta tcgttatatg agaaaaaaac attccatttt agtaaagaat
    71641 gggttaccag aaggaggaga atggaactac gataggctta atagagagcc tttcaaaaaa
    71701 agagataaaa tcccaaagag aatagatttt tcactcaatg cagttacaaa agaagtaatt
    71761 gaattggtaa attctattta taaaaaccat tttggaaata caaatttatt caattatgca
    71821 acaacaagac aagaagcatt ggttgcactc gaatttttct gtaaacattt tttgaaagaa
    71881 tttgggaaat acgaagatgc aatgtcaatc caagaaccaa atttatttca ttctctactt
    71941 tccccttatc taaataattc cctcttaagt ccgatggaat gcattcaaaa aggaattgaa
    72001 tactacgaaa aaaaagaagt tcctttaaat tctatagaag gattcatacg acagattctc
    72061 ggatggagag aatatatgag attagttttt atatcaaaca gggaaaacta tttacaagaa
    72121 aatttcttta agcacacatc aaaacttccc tctttctact gggaaggaaa ttcaggtatg
    72181 gaatgtctag accaaaccat caagcaagtc attcacactg cttattctca tcatattaca
    72241 agactcatgg ttctttctaa ttttgccaat ttacttggag tgaatccaaa agaaatcaac
    72301 gattggtttt ggtctatgta tatcgatgcc tatgaatggg ttgttgtgcc taacgtcatg
    72361 ggaatgggaa ccttcgcaga cggcggaaaa atttcaacca aaccttatat tgcatcagcc
    72421 aactacattc aaaaaatggg aacagaattt tgtaaaaact gtaaatacaa accaaaagaa
    72481 attttcacag aagatgcttg ccctttcaac tctctctact gggattttat tcacagaaac
    72541 aaggaatatt actccaaaca atttagagat tactcttatc taaatctctc taaaatggaa
    72601 aaatccaaat taaataaaat tttagaaaaa gcaaaagagt ataaagtaaa atttttagat
    72661 aaatagaaag tgggagttga attgggaatg catcgagcta acttattgaa ggcgaaggtc
    72721 tgccaggttt cgtccatcag acgaaagggg gggaagtaac tatcttcgcg aaaagttagt
    72781 tactagatcg gtctactttc ctttttaaaa aaaatgatcc agccatatca tcgcgactga
    72841 gcgatgatgg gacattcata gc
//
LOCUS       DAOQOS010000009        71859 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_120_length_71859_cov_6.509568, whole genome shotgun sequence.
ACCESSION   DAOQOS010000009 DAOQOS010000000
VERSION     DAOQOS010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 71859)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 71859)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..71859
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_120_length_71859_cov_6.509568"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            249..1268
                     /locus_tag="PLF66_03245"
     CDS             249..1268
                     /locus_tag="PLF66_03245"
                     /inference="COORDINATES: protein motif:HMM:NF012907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HMZ63084.1"
                     /translation="MIIRYLLIFITQSILWILFLSFSIFAETNKPDSIKTETAKGKWG
                     YILSSSLPKFPIEYLETISKEYEILCINGLELNGKGQLRYTSQFIDKIGKSKLKTKDQ
                     NRAILYPMITVSSIREGIELLNSDKSKRKATDQILEFLIENKFPGVHLDIEYLPSEFD
                     LKLSDFLALLKKNLNDNNLRLSFAVFPQIDFPSSISKLHNVEKLANDVDEIVLMSYDR
                     HNKKTEAGCVTSLDWSKKNITHLLKFFKPEQLWLGMPAYGYEWIIETKKVNIVSSRDG
                     ELYNKTWEGKRESSGCLKILKEDKKKTSLIYFADEETRNKMREVAEEFKIRGTAIWRL
                     GLEDN"
     gene            complement(1271..2014)
                     /locus_tag="PLF66_03250"
     CDS             complement(1271..2014)
                     /locus_tag="PLF66_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016549369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HMZ63085.1"
                     /translation="MSGHSKWATIKRKKEAIDAKRGAVFTRYAKEITVAARMGGSDPE
                     ANPRLRVAVLKAKSANMPKDNIERAIKKGAGELEGVTYEDCLYECYAPGGIAIMVSVT
                     TDKKSRTTPEIKNILTKFGGSLANAGAVSRLFEKKAIIIISADQISEDALVDIVVDAG
                     ADDVVLQEDVFQITAQPEAYEKIQEAINSKGLQVIESEVKFVPSVTVEISDPLVAEKV
                     MKILDAFEAHDDVQNVNSNFELSPDLNMG"
     gene            2225..2413
                     /locus_tag="PLF66_03255"
     CDS             2225..2413
                     /locus_tag="PLF66_03255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63086.1"
                     /translation="MAYWQGRLRVQGFKKQRSKTVQPAFLPNLATKFNPFVVSNWLFR
                     NRYMYALANGCLPLGEII"
     gene            2410..2739
                     /locus_tag="PLF66_03260"
     CDS             2410..2739
                     /locus_tag="PLF66_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016807542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HMZ63087.1"
                     /translation="MKWIYLTIAIIGEVIATSALKSSEGITKPIPSTIVIIGYGLAFY
                     SLSITLQSISVGVAYAIWAGVGTLLITLVGYFFFQQKLDMPAVFGLTLIVAGVIVINL
                     FSKSISH"
     gene            complement(2837..3865)
                     /gene="pheS"
                     /locus_tag="PLF66_03265"
     CDS             complement(2837..3865)
                     /gene="pheS"
                     /locus_tag="PLF66_03265"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001053743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HMZ63088.1"
                     /translation="MSLLDDLNAILKEANDVLPTSKTEIDLDTNKNLFVGKKGKLTGV
                     MKGLGALSPEEKKTIGARANEVSKEIEDLVNKLKQKIKLDFYENELKNEKFDTLRPLQ
                     SRDLGGLHPLSRIQYEVEDIFTSMGFQIMDGPEVETDYNNFEALNFTSDHPARDMQDT
                     FYTKDGNLLRTHTSAIQVRALRNLKPPFRIIAPGRVFRYEEVDASHENTFYQIEGMVI
                     DKDVSTANLIYTMNVLLTEIFKKEVKVRLRPGYFPFVEPGFELDISCMVCGGTGCSVC
                     KHSGWVELLPCGLVHPNVIEFAGLDPKVWRGFAFGLGLDRLVMMRYAINDIRYIHSGN
                     LRFFKQFV"
     gene            complement(3865..5337)
                     /locus_tag="PLF66_03270"
     CDS             complement(3865..5337)
                     /locus_tag="PLF66_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002971896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HMZ63089.1"
                     /translation="MNIYMIGIGGIAMGNLAFMLKQSGHNVFGSDQKLYPPMSDKLEE
                     WGLKTFEGFSADNVGKPDLVIIGNAISRGNPEVEYVLNEGIEYMSMPQAIGHFFLKKK
                     KVIVVAGTHGKTTTTFLIHHILKQAGLKPGLFAGGIRKDGNAGFELGGGEYFVIEGDE
                     YDSAFFDKSSKFLHYRPYYLILTSLDFDHADIFPNLDAIRVMFKRLLNLVPSIGRVFY
                     WEGSKNLTEICENFKHAPVQKYEVGKKDSILRIGFLTRNHVKEEKAFSAKTGRELKSP
                     LIGMHNYRNTEVTANLCKAIGLTEEEIFRGIESFPGVKRRQEILFETESAIVMEDFAH
                     HPVAIEETIKAVKTNYPEHGIISLFEPRSATSHRNIFQKEFAESFKKSNVAIITEVFN
                     VNKVDKKIRLDVKKLVKEVPKKSKIKKAIYAKDTNELLLKLEDELENYESKFVILAMS
                     NGSFGGIYPKIIEIVTDRVREMQKESFKEKTGKSKKKGKK"
     gene            complement(5339..5938)
                     /locus_tag="PLF66_03275"
     CDS             complement(5339..5938)
                     /locus_tag="PLF66_03275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63090.1"
                     /translation="MSQESIDKKEIQLLSDIFASPKDAFSSYIQNVNFSRFSVFRIHL
                     FLFVLAPVFKLLHNLFFTFVGSVHFGWEAPPKLTDGITTAIAVYPLVLFLVYHFDILL
                     LRLRTGKASIDVIEERDLLLISFLPFSASAVFWIFPKPFNFFLILSAFIYCLYLSKTS
                     LEMFFGFTIKQFIVFVSYILILCMTLSAVALTIFNLIRK"
     gene            complement(5990..6451)
                     /locus_tag="PLF66_03280"
     CDS             complement(5990..6451)
                     /locus_tag="PLF66_03280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63091.1"
                     /translation="MSIFRVNLYNTFENIIKRKAFSAPSSTHRVYKDLSDLSKWETLE
                     KIKKGNLILLCRGIRFVWAIAHASDDCRITDVDAFRPAMKFEKNGFRFVSLDIYKEYA
                     SPLDGKKFFNNLVYEHPFEDPNFCVEIKPADGLEDAFNTAINKLLEDAILS"
     gene            complement(6550..9693)
                     /locus_tag="PLF66_03285"
     CDS             complement(6550..9693)
                     /locus_tag="PLF66_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HMZ63092.1"
                     /translation="MIIRFIGIAIQHRIPTLLVGIMTVILGIWAWTDLRKEAYSDIAD
                     TQVRLIAKFPGRAAVEVEERVTLPIERILNAIPKVIVRRSRTINGLVVFQFVFEDGTD
                     DYFARTRLLEKVKEADIPADVTPQLAPMSSPVGEIYRYVLESSENHTAMELRTIQDWI
                     VMPKLLQIPGIADVVTFGGLLKQYHVVTNPDKLIRYQLEVGDVIQAIQDNNLNTGGNI
                     LRQGGQGFAIRSLGAIREIKDIENIVVKQHDGVPIFVRDIATVEIFPSLPTGVLGYVF
                     QNQEDGSPRVESDSSVQGLIAMRRWGDSNEMGEKIRAKVKEINENYLPPGVKLRNTYD
                     RSDLVKYTIGTITKTLIEGVAIVTLVLIFFIGSIRASLVVVTTIPVSMLFAFIMMDLT
                     GIPASLLSLGAIDFGIIVDGAVVMVENIMRRYRDADVEEKQIGINPFTASASAEVGSE
                     IFFSITIIILAYLPIFSFERIEGRLFKPMAFTIAYAILGSMIFSLTVIPVMMTMMYKK
                     YFESANPGPIEWHNPVYHWIEHKYSNLIVELIRRSKQTVTVAFSIVVLIVGIGVWKIG
                     SEFLPELDEGGFNIRAYFPVGIHLQEARKVIPKMREVISKNEQVNVIITQLGRNDDGT
                     DPLGPNRLEILIGLKDYSTWTEKITKQELLIRMKNDLEINCPGVRFSFSQPIMDNLSE
                     AITGTIADLAVFVSGTDLDVMRKIANDILAIVKDMEGASEYGIEQEGNSTQLSVKINR
                     ESAARYGINVSKIQQMIEAAIGAQRISTLYEGPWRFGIVVRFSSEYRSTVKALENVPV
                     ISPSGQRVPLSQLAEIKLIDGPTMIFRQEGRRNVSVRTNVRGRDQGGFVTELRKKVDA
                     HIKLPDGYKIAYGGQYENLNRVGKKLAVVIPVTIAIIFGVLFIMYRNLRYVSVALACI
                     PLSLIGGIIALLIRGYYFNVSAGVGFISLFGIATMAGVLFVSRTNHIMRDNANITIEE
                     AVGRAAIIQLRPMLMTMLLALLGLIPATLASGVGSDVQRPLATVIVGGLTSALIFILT
                     VLPSLYLLLVKKRKEEY"
     gene            complement(9721..10380)
                     /locus_tag="PLF66_03290"
     CDS             complement(9721..10380)
                     /locus_tag="PLF66_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017857798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63093.1"
                     /translation="MQENEKSIEKVRSDKEKEPLTGYDPETNNKKSIRDNNEKYSNFW
                     VVGGTIGSPASANFNVGYYFTNFVLRASGMRYSQHWNGIQGDIGYSFWKTSVIAHSVS
                     LVFGEMNVRPFDPQTQQGGQNKYDRDGIPGYNHQPPTFGDTLIRSYIAEQNPTLATAL
                     EYQYRTRQYANFHQQYVGLTYDILVGGFFLQVGMGYGRGDYRNPQLLLQMGYLFDFGR
                     N"
     gene            complement(10676..11641)
                     /locus_tag="PLF66_03295"
     CDS             complement(10676..11641)
                     /locus_tag="PLF66_03295"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HMZ63094.1"
                     /translation="MKFKNLFIFINLILIPFVFACKKKQVEPQKNYRPVVSEEGQKIK
                     FDPESPGLSQIKVSKFGEGDGFISISAPSRVIASITNSVSGGRIVLFESPDINAIYAS
                     YQVSRTSLTRASKNLNRVQDMFKNQVATQKDIIEAEAAVNSAQAETAEFEGKLRAIGF
                     NPSELQFVGSNLVWLICDIPEANLSVVRKGKEVKVHFASFPDLTLKGKAEAIGDNVDP
                     LTRTVKVRVALPNKEGKFKPGMFAKVEFGDETKDIAVIPFSSVVTVEGQAYVFVQTTP
                     GEFIRRPVVLGNSGSDNVSVVQGLFPGEEVVIGGAMLLKGLSFGY"
     gene            complement(11655..12974)
                     /locus_tag="PLF66_03300"
     CDS             complement(11655..12974)
                     /locus_tag="PLF66_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012387600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HMZ63095.1"
                     /translation="MWIFYKIKNKNAFLSLSFLFFSFSQILLSQESTKNYSLTLKEAE
                     ETFLKNNLRLLAAKFEIDVKKAGIIQAKLYANPNISVDQNIFNDNTQRYFDTTRNGQT
                     TLQIQQLFLLGGKIEKRTKVAEINKAISEQQFFDLLRALKFELRSSFFGIHYLRQSIQ
                     FYDGSIAALTKTVSSLEKAYEKRAVLQSEMLRLKALLFFLENERTEAVVQVKQKEASL
                     RILLNSPELMNVTFIPKIDSKGLEEVRLTDLKLDKVINDAFENRPDLKIAIQALKLEE
                     ANLALQQANAVPDLSFGPMYNRNGTAYPNYWGVTAQLTVPIFDRNQGNIEAAEKAILT
                     RRSELQNQKLQAENEVTIIYNRLLEKDRLYQAFKNRLTEDYTNLAQLMISNYEKRYLT
                     VIEFADFFETYRTSVQQMLKLQIDRVDSIENLNYSVGKSILNIQPNE"
     gene            complement(13055..13396)
                     /locus_tag="PLF66_03305"
     CDS             complement(13055..13396)
                     /locus_tag="PLF66_03305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63096.1"
                     /translation="MKIKLIKNLSIFNILISLFLIISLIPLNLANPEEDYSLQSNTEY
                     SDVISDYSLYSTMRVQSRAGDINDQYFSDRFYLVSIEQFHYDDTLILEEDSIPARLLC
                     YRLISLPPPTV"
     gene            13638..14147
                     /locus_tag="PLF66_03310"
     CDS             13638..14147
                     /locus_tag="PLF66_03310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63097.1"
                     /translation="MNKMNLSLITTLLLFYCVSQDKKKVEIDDTFYKDCMETIKEKEK
                     CDALVKKAEDREKEKEAKQEKLTEEQMAGLEIRDDFKNRMMGKSKLGVISSIGEPDER
                     IYDGSGQEFFHYKKPLTRYSPEHDPDKEIIVVFRREYVTRVLHTPPDSTPKSSFPFIG
                     DKPKKPNSK"
     gene            14444..16036
                     /locus_tag="PLF66_03315"
     CDS             14444..16036
                     /locus_tag="PLF66_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020771634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HMZ63098.1"
                     /translation="MKTKNIILCLLIFSAAGISAQTITPKGSDPKPAEAKWYDKVNFS
                     GYVDVYYNYTANNRQGSTQDTTGTFHTYNKQFAVNAVKLSVEKLPEKETPWGFRLDIQ
                     NGQNIMYQERPYQTSNSIYNMQLLQQAYVSFYFPVAKGLVIDAGKMATHIGNEVLDSK
                     DNLNYTIGYIFFNTIPFIHTGARATITLTDKLSAGLYLYNSAQGTGYTANGQQFGFAG
                     TTPVGPNGATINGAPQAGLLTSPNQSISGNHVYVDGPNPMKSIGTQMKFDAVKDKFKI
                     VWNTLYGNDVTQGRPSDADFYLSQSGMNGRVPGGPLLTQPNQVSKNNRDYWFINHMSF
                     IINPTDKFSILLDWTFGERSGYAITNAAGYTNEGFYIDSNRDGVIDTVNGTDLKYRPS
                     KQDVKRIYNTYGIWAKYTINDKYAIGLRYENIDDSRYGGSLAVNAPLAFTNPRDRYDL
                     QAASGDSGFTNSRYAGLGRPASGLGQARTLTLTPIINFTENLQVKIDLRRDWALGTQF
                     IDPSGRAVKGQNGIIIGVVAKF"
     gene            16053..16574
                     /locus_tag="PLF66_03320"
     CDS             16053..16574
                     /locus_tag="PLF66_03320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63099.1"
                     /translation="MKTRKTLIYVAMGLMLSILANCTESSKAGNDTDKNNALLLAILN
                     RNTANAQARQACANAVYLMNQCIGSGSGFNPAVMCADANLQAGGTYTDSSVTPAATIT
                     YTGTEAYTRLASCVATQIAATNCNFSQNKVASPVQAKAAFFSSCEVDYNSASITAATK
                     ATKAPSGTIITIF"
     gene            complement(16620..17924)
                     /locus_tag="PLF66_03325"
     CDS             complement(16620..17924)
                     /locus_tag="PLF66_03325"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HMZ63100.1"
                     /translation="MENISIASYLYLSFSFGLLSLLTPCVFPLIPITVSYFTKRHELS
                     SSKPFSDALLYSIGIIFSFSILGFVIALIFGASGLNRLAANPYMNLFIAVVFIIFAFN
                     LFGMFEIVVPGGFLTRISNFHSENNLLSIWIMSFTFTLTTFTCTMPFIGTVLVSASKG
                     EWFYPILGMLGYSFAFSIPFFLLALFPSGMKKLPKSGSWMVSFKIILGFLELAAAIKF
                     ISNADLVFGWKILSREFFLSIWGAIFIATSLYLFGFYNFAHEKKEDGKTAFRIASAIF
                     FLALALNFLTATNGRSLGELDAFLPPPSTELKNGVTKSEELIWLDNLEVAKQEASKTG
                     KKIFVDFTGYTCTNCRWMEQNIFPEADVKELLSRYILVRLYTDGEEMVHEENQKFEED
                     NFGTIALPLYVGMNADGKVIDQFLGMTRDKEEYKRFLERMLK"
     gene            18264..18548
                     /locus_tag="PLF66_03330"
     CDS             18264..18548
                     /locus_tag="PLF66_03330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63101.1"
                     /translation="MIIQNKNKGNLIYLLSFLIFLSLFCNTEDRKPKEAIVYLKDNTD
                     GTIEDTRYNYTWQKCSMGQTQNEICAGSAVLTNWLSAMQYCKHLKLANKK"
     gene            complement(18872..19213)
                     /locus_tag="PLF66_03335"
     CDS             complement(18872..19213)
                     /locus_tag="PLF66_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016546707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HMZ63102.1"
                     /translation="MKLIIAIIQPDKLDEVREALLAAEITRISVNRITGHGREEDTEL
                     FRGQRVKPNLLPKVEIKIACNDEFEDIVVNAIMNSAKHGDGVVGDGKIFVVPLEKCYR
                     IRTGESGKTAI"
     gene            complement(19225..20271)
                     /locus_tag="PLF66_03340"
     CDS             complement(19225..20271)
                     /locus_tag="PLF66_03340"
                     /inference="COORDINATES: protein motif:HMM:NF013104.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HMZ63103.1"
                     /translation="MEVVDSVTTAWMLVSTGLVLLMIPGLAMFYGGLVHTKNVLGTMM
                     HSFVPLAVIGVLWVAVGYSLSFGKNALGGFIGWNPDYFFLNGIDESIVNGVPEYVIAM
                     FQGKFAIITPALISGAIAERVSIKGYILFIALWFLAIYCPLAHWIWASDGWLFTSGPG
                     VIDLAGGLVIHVSAASGALTWMIVESIKFRKVTSLGFVSGILAGLVVITPAAGVVTPK
                     GAILLGMASSIICFFALEMKNKLGYDDSLDCFGIHGVGSGFGVLALSFLIRDSWMKQA
                     AAKMGGEWTYFQQFLLQLKGMGVTILLSAVGTLIICFIVQKTVGFRISKSEELAGLDH
                     TEHSESGYGLMNLN"
     gene            complement(20722..21102)
                     /locus_tag="PLF66_03345"
     CDS             complement(20722..21102)
                     /locus_tag="PLF66_03345"
                     /inference="COORDINATES: protein motif:HMM:NF012982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dual specificity protein phosphatase family
                     protein"
                     /protein_id="HMZ63104.1"
                     /translation="MIYKDLPNDIEIVVDLTAELNEDYSIIQNKKYISFPILDASIPD
                     EVDFYLFIKEVSKLRSNIYIHCAEGHGRTALVAIILYLIRNKKQSVEEAFEFIKGKRR
                     KIKLNKEQESFLNKFVKSEYFLNI"
     gene            complement(21461..22390)
                     /locus_tag="PLF66_03350"
     CDS             complement(21461..22390)
                     /locus_tag="PLF66_03350"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HMZ63105.1"
                     /translation="MKPIEDEFGLKMLIGNSSPIKSLNVKIENLSELNVPVYIQGDVG
                     TGKSLIAKLISQKEMIEIDCGSFHSDLNILIHKYSEKTAKTLHIKGESIKINSPVYFD
                     KLELLDTDGQAILFRILENQEIVTDKKETVQFASRIFTSSNKSLPQMVKDGIFRKDLF
                     QKINLICLNTVSLKEVKSDLPLLVNHFVNELKDKYNKNITRISEKLIQFILQYDWPGN
                     VAQLRNMLEGMIILSSEKVLDVSQVPKDFITTSSIITRGKLNIIPGIPMNDYEKEIIR
                     ENLKVVGGNREKCAKVLGISERTLYRKIKEYDL"
     gene            complement(22391..23308)
                     /locus_tag="PLF66_03355"
     CDS             complement(22391..23308)
                     /locus_tag="PLF66_03355"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HMZ63106.1"
                     /translation="MNQYKIILLFSFLFIGCVSNSKYDTLKQTCDETEQKLQEELKAE
                     KEKSIKLEDDKNKEIANLKEAQSKELQAKQDKYNELLKEKIFLRSSIAQMEGSVDDLS
                     QEKDALAKQRDEMTRALEDLKKRKEESEARVTEFKSLLDKFKTFIDTGKLKIKMIDGR
                     MVIVLPSDVLFASGSKDLSSNGKKAITEVTSILTEIENRKFQIEGHTDSDRYRVYGQT
                     NWDLAADRAINVLYTMIKAGLPESKISAASFGDSRPIAPNDTNANKALNRRIEIVVVP
                     DLSLLPGYEELKKMADTNGSQQNKISKQN"
     gene            complement(23365..24114)
                     /gene="map"
                     /locus_tag="PLF66_03360"
     CDS             complement(23365..24114)
                     /gene="map"
                     /locus_tag="PLF66_03360"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HMZ63107.1"
                     /translation="MSINSMRDIQLLKKIGMIVSSVLREMRQAARPGMHTEELDYLGG
                     KLLEKKGARSAPKLTYDFPGNTCISVNDEFAHGIPSSRRKLAPGDLVNIDVSAELDGY
                     FADTGASFQIPPYNAMTQRLIRVTRSALDSAVNIARAGRKINQIGKTVQDLAQNSGYT
                     VIRNLGGHGVGGALHEEPRFIANYYDKSDDRVLEDGMVITIEPFISTRAIHAVTKSDG
                     WTLRTPDSSLVAQFEHTLVIRKGYPVLITQL"
     gene            24430..24759
                     /locus_tag="PLF66_03365"
     CDS             24430..24759
                     /locus_tag="PLF66_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017861432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HMZ63108.1"
                     /translation="MNIYVGNLTYEASEADVRAAFEAFGEVTSVKIITDKFTGQSRGI
                     GFVEMANKTDALAAISDLNGKELKGRMLKVNEALPKKSFDKGSSNNSYGNRPNKGDRP
                     RRNNNWR"
     gene            complement(24952..25707)
                     /locus_tag="PLF66_03370"
     CDS             complement(24952..25707)
                     /locus_tag="PLF66_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HMZ63109.1"
                     /translation="MPKEKTRLDILLVERGICDDLARASSLILSGSIIVNGEKVTKAG
                     FKYPRDVKIEVVDKIPEYVSRGALKLKSSFKAFNVNSNKKICIDLGASTGGFTEILLL
                     NGAEKIYAFDVGYGQMASRLQNNSKVILKDRFNVRNLSWRDLDFNYSDLFIVIDLSFI
                     SLLHIFPVIYQLKQDALDKKIEVISLIKPQFECEPEETEKGIVKNHRVHFRVLKKIIS
                     YLKYQMKAEVIGICNSGIRGASGNREFFIYWKL"
     gene            complement(25688..26593)
                     /locus_tag="PLF66_03375"
     CDS             complement(25688..26593)
                     /locus_tag="PLF66_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015682591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HMZ63110.1"
                     /translation="MEEFLNLFKIRKDRFENFFHEVIFNEIRKKFHPLLAEACIYSLS
                     AGGKRIRPVLVLSAYLSSENQSISNNVLFLSSAVECIHTYSLIHDDLPAMDNDDYRRG
                     VLTCHKKFSESTAILAGDALNSFGFYLPAKMEIEDSNTFSDIQELLHSGAGGEGMVSG
                     QIEDLQLENNPNFFSEDTLNSIHRKKTGALILSSLLIGNRLTKEWKEKEKNFQLYGEK
                     VGLLFQITDDIIDEESSFEELGKTPGKDAANGKLTYPSLYGMEKAKRLRDDLKGELIA
                     ISESLESNKSHFFRDLPVYIAERKN"
     gene            complement(26606..27022)
                     /locus_tag="PLF66_03380"
     CDS             complement(26606..27022)
                     /locus_tag="PLF66_03380"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002771253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HMZ63111.1"
                     /translation="MSRTFIMIKPDAVRNKHVGDIIARIEKEGFKILAMKYVKLAEND
                     AKEFYKVHAARPFYADLCKDMSAGPIVVAALERENAVSHWREVIGATDPKEAKPGTIR
                     AMYAESKGANSVHGSDSDENANTEITFFFKGFELVN"
     gene            complement(27036..27536)
                     /gene="coaD"
                     /locus_tag="PLF66_03385"
     CDS             complement(27036..27536)
                     /gene="coaD"
                     /locus_tag="PLF66_03385"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010573289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HMZ63112.1"
                     /translation="MRKLAVYPGAFDPMTNGHIDIARRALNLFDTVIIAVATNSKKQS
                     LFSIEERIDLIHHVITDLGIDESRLEVTTFNGLTVDFCEERGASAIIRGLRAVTDFDY
                     EYAISLMNKKLAPELETVFFMASGDNSFVSSSIVKEVARHGRSVAGHVHEIINEALLK
                     KFKELK"
     gene            complement(27533..28747)
                     /locus_tag="PLF66_03390"
     CDS             complement(27533..28747)
                     /locus_tag="PLF66_03390"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015682288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HMZ63113.1"
                     /translation="MKKEIAPKKIVLAYSGGLDTSVILAWLKDTYGCEVIAFCADVGQ
                     KEELSGLEEKGLKTGASKVFIKDLRLEFARDFIFPAIQGSAIYEMRYLLGTSLARPII
                     AKAMVDVALAEGADAFAHGATGKGNDQVRFELGFKALAPSLAIIAPWRTWSFKGRTDL
                     IEYAKQKGIPVPVTAAKPYSMDRNLMHLSFEGGILEDPYKEPDPEMFLLTVSPEKAPD
                     SPTYLELDFEEGNCVAVNGKRMNPLEVMESLNKFGGENGIGRVDIVENRLVGIKSRGV
                     YETPGGTILHIAHRDLESITLDRDTQHQKDDLSGEFARLIYNGHWFSNKMKSMRAYIS
                     ETQRFVTGTVKVKLYKGNCSVVGRKSSVSLYSHEMATFEKEELYNQYDAEGFINIYGL
                     PTKETSRLRNKK"
     gene            29335..30912
                     /locus_tag="PLF66_03395"
     CDS             29335..30912
                     /locus_tag="PLF66_03395"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HMZ63114.1"
                     /translation="MNLNTESNKNDSIDRGRLLTLLIISSTCTGAGLGWGGIYAFFGL
                     YKAMWWPFLFSFVVGGALLAYRFFKSYNLLLHTQLFMIFIVPTLLQWTLGGFHESGIV
                     ILWSLMSPFGSLMLQGKKEYLSWGGAYFLLLTVSLIFDGYFKSLGIESVSPNAIVFFY
                     AMNISVVSLLTLLAIFYFVKSFEDERTARTSYNNYLSDRVDTMLKSVELLADGDLTSK
                     IHVSEEDLVIQKLFTGYNRALEVLTKSFDDLDKNIAIVANSADGLIESMDTLSSEISQ
                     QTVGVTQIEDFIKKIKKETEENSKLIQLGAKESEANADIALNGVSIIEKSISKIQSIG
                     KEMENSRFIILELEKESNQIDEIIHSINGIAKQTSLLSLNASIEAARAGENGKGFAVV
                     ASEIGKLADMTTKSTKLISDKLKEINQKAKHAVDIVNKSNENMREELTYSGQVNKSNQ
                     NIVTNSKRVREIISTLEERSKKQSSNLQDISVNIIQLLDSTKFFLNEIKTMSDSFLSM
                     NQKTKTMKDSVKKFKIA"
     gene            complement(30909..31613)
                     /locus_tag="PLF66_03400"
     CDS             complement(30909..31613)
                     /locus_tag="PLF66_03400"
                     /inference="COORDINATES: protein motif:HMM:NF014543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63115.1"
                     /translation="MLSFILLLLLFTSNVFSDSCVSGNCEDGHGIKEFSNGDRYEGEF
                     KKGIFEGEGIFFSNKGYKYIGKFKEGKRNGFGIWTKENPIKPSGERISVSEDLAVNKS
                     IYYEGEWKNNKYEGNGKLIIKKNGKTKIYTGKFVNGKLNGQGKLETLSGKYSGVFKNS
                     KLNGHGVFEKKNGESYIGNFLNTKFDGKGAYTFKNKDLYIGDFKDGKLHGRGRLFNSK
                     HRVIQSGKWENGEFKN"
     gene            32079..32594
                     /locus_tag="PLF66_03405"
     CDS             32079..32594
                     /locus_tag="PLF66_03405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63116.1"
                     /translation="MKKTFLILGLSLSAFLINCSSRQTILSPSKEDRTYDYTSCGISF
                     TIPAGWELQTTPFLELHVVKGNIDIIIDPVAEETDPDKIWTAVKDKLSDKAGKPLKLS
                     RTEKGKKVSGIPSGTIYSMTEDKKISVDGDVLQCPKDKSVIFYSISPVATYRTDRPAV
                     QRLVGSIKPLK"
     gene            complement(32701..33255)
                     /locus_tag="PLF66_03410"
     CDS             complement(32701..33255)
                     /locus_tag="PLF66_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008590582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1569 domain-containing protein"
                     /protein_id="HMZ63117.1"
                     /translation="MNRRKFIQKAGAFTVTTYALSANMNCSNEVDKMDRKLRFSNLEE
                     SINELKKIETAKKITPNGSWSWFQILNHCAQSIEYSLIGYPENKSPLFRMTAGKIASN
                     LFSARGYMTHDLNAPIPKAPEIPKEGDEREAMLRLYKAIEDFRNFTGDLKIHFAYGEL
                     SKSDYEKAHAMHIANHLSFVEVDY"
     gene            complement(33248..35113)
                     /locus_tag="PLF66_03415"
     CDS             complement(33248..35113)
                     /locus_tag="PLF66_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002748203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF115 domain-containing protein"
                     /protein_id="HMZ63118.1"
                     /translation="MNELLLKNISLLSDDIKEKIVKSKDLGSLEKTKSGAFTLLVEGI
                     YFHSKHDPLIEAKRLIENLKKTNEEKVYIFFGAGLGYAIQEALKQPNIQIIWMECYPE
                     ILKQALSTIDFSNFIKSGQLKFLLKPFTEDTLFSTFKGISVLPVTFLPNRPSLSWKES
                     EYIECKYICEKFFQKKDVNIATLTKFEKIWTRNLIQNIPEVSHLTPVSRLFGIAKGLP
                     IVVSGAGPSLYYNLEDLKKYRDKFILIAVDTSLHILDRSNIQPDLIYSVDPQAINTSY
                     LEGYSGTGLIVFDPTSSYHTLRLSKIFKKGFFTSSPFPLLQIYTKHLSCEVGDIPFGG
                     SVSTNAVSLAELMGASNVYFVGQDLAFTDGFAHCKGAILEERLNFKESRYFRREKHNY
                     NQLHALPKLKSIGYNEEEYHSNEKMQIFNKWFSDRSSGKNWINLSAKGARIEGVPKKS
                     FRDCFESINEEVPLKVSNVRAMIRDLSKEGQKSFFIKNEFLSETIGIINELTLFKNTL
                     KKGKYIAEKIYSLIEKGNQAKDINYLLSQMDKIDEEVSSKKNLNEIIGLSIQRTILTI
                     TEGYEANLSLPEKKDPHLGVARKSILLYTGLYHGASMIEKLLKKVLIRMSEINNE"
     gene            35337..35903
                     /locus_tag="PLF66_03420"
     CDS             35337..35903
                     /locus_tag="PLF66_03420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63119.1"
                     /translation="MKKLFNIFQYFHYTLLIYGLVISLTAIYFIGAFHYYTIENNLKV
                     EEVIYSNLLKNEIREKIEKAIKTNRLSNFEKEWEPYMNRLAIMIQDYYNFKMYFDSHE
                     KYKFIQLNESIDNYTITNADNKYEINIYSIEGKSWDSIYIHWLNYFLKSFRPTNKYRY
                     LTNWLLIIYPVSILILIFYRAKRNRLTP"
     gene            35923..36264
                     /locus_tag="PLF66_03425"
     CDS             35923..36264
                     /locus_tag="PLF66_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008592340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF962 domain-containing protein"
                     /protein_id="HMZ63120.1"
                     /translation="MENKTYNTLAEFWPFYLSEHSHKTNRLLHFIGSTFALLFIFMAI
                     QSSDLYFLLVALFSGYGFAWIGHFVIEKNRPATFIYPIKSFISDWRMYFYILTFQIDK
                     ELKKYSIESKG"
     gene            complement(36261..37226)
                     /locus_tag="PLF66_03430"
     CDS             complement(36261..37226)
                     /locus_tag="PLF66_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004763882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HMZ63121.1"
                     /translation="MAKPLKIEVPLLIKKFNKQLDSIIKEDLPILKDIKEKVIESGGK
                     RIRPLTHYFLTQLLGYDGKQWLDVGTIAELIHAASLLHDDVIDGADMRRGKPTIGKLY
                     GNKTAILAGDFLLACGIHHLNSLNNPELMSAFTVVIRDLSVGELVQMEWEKNEKITQK
                     EYNQIIYGKTASLFGAVGVTAGILHDADSKMKKKLHSFGINMGMLFQIKDDYIDYFQS
                     SENSGKMQMKDFVNGLYTYPLILLKEKANKKDFIEIKQILSQKERGQNEISRIQVLLS
                     QYEIQKQIRGNLLNLSQDLIGFLKTFKESSIRKLMIEKIEELVED"
     gene            complement(37318..38301)
                     /locus_tag="PLF66_03435"
     CDS             complement(37318..38301)
                     /locus_tag="PLF66_03435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63122.1"
                     /translation="MRLFVLFSIFIFNSLLADMVILKNGQVLIGSYLNEDDKKIKFQV
                     FKEIKTIAKSDISSFELGYSGVPVCYQLQNKWSQTCGDVIHILDKNKMVLGKGAGLLE
                     KEELQLKDLKKITLKKSKKEERIFFILRPGLNLTVSANGKNYTGEIQSVNFETMSIKT
                     KSETVSFKENDVEEVSWQGSSKASLAFLRYVVPGLFQFQEGKRFKGILLGFLFLGFAG
                     AIGAEFSAAQSALNNDTDYIIINNSLYIGSNIFPNQQYDKHIRGMNYAIAGLTLVYLY
                     HSYEVYSFTTSKGVKASIHMQMPSISSQAFTPDIVQGKRSNGIEFRISYSF"
     gene            38676..40208
                     /locus_tag="PLF66_03440"
     CDS             38676..40208
                     /locus_tag="PLF66_03440"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HMZ63123.1"
                     /translation="MEPVLTKIKSSRSLRLQIGILYSFLALVNILFFSVMIFENQTEL
                     LVKNFKYQSENFVASVMTDLNSKRVIKADDSSIKALKENLFYVEIYQFRIFDITGEVW
                     YNEKGIVSNEKADEDLIKKAKEISDTTSVFASKYKLELNKEDFTIKFLIPLVAEPEEA
                     GPIFLSTQLSIKTMQERLTKLYYQIAIAFIWGLISHILFGIYIYRVIFTRVSILKDAT
                     DKMALGDLSSRAQWKFNREDELDVLGQAFNGMAGKIEETIRTIQNLNDEITKELKIGK
                     EVQEMFLPANKKLKNLKVATSYRPMREVSGDIYHFYKFPAGKMNKKEYQGIFFADASG
                     HGVSAALVTTVLIMSLENILLKSIHPGYIIQKLGDIIGNRFQSSFFATAVFFLIAEDG
                     EILCSNAGHNAPICFRPSTGEIFYFKSVGPPLGMVDEFDYKVIKMPAKSGDKVFIYSD
                     GLVESPGQNEEQFSEKRVLDIIEANMHIPNTDLLKLLAEELDKFAIDYRDDVSMILVE
                     IP"
     gene            40209..41756
                     /locus_tag="PLF66_03445"
     CDS             40209..41756
                     /locus_tag="PLF66_03445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63124.1"
                     /translation="MQRKILILFFLLILILFGMASRAIVDVKIQELRFQLTKEQLLNY
                     ELSSKVLKEKIRQMMLTKDDYTNEIKVNILESSVMNAQLGETTTKLSLIEKFGLGIVN
                     IVRVISTKSPLTLEEDQNDMMKIQFGFYMERTRKFATALKKYQELEKTLRNKESDEYG
                     FIMLHSGFCIAMTGDTKGAIEKLRQTEDMFVGSHFADNARILINVLLEGERKINDINS
                     GTKTESEKANLLYETGQYKETLAQLEKIENRSNDQNYMRARSLEELGQTSKAISEYLS
                     LVEKKEVDKDVAVKANRRLLLIGSIYEKNDDLTDYSKSNAKQLGDTDIVKKVDEGSSL
                     LLKSLIVEKLTTEDAKTADPETMKEMVELKKELETIKLKDKEERKESIEKVEKEVAVL
                     KAEKPPEPPPVPEEKYDSTESLIMKLSLSDGRSIQGKTILFEEDKMSVSINTFSTKMP
                     VTLLSEISLNQNLKLKTPPLAIQLKSGKVMLGYKFRRESGDTFLLNDKRKDFSIDLDD
                     IKKISIQ"
     gene            complement(41758..42087)
                     /locus_tag="PLF66_03450"
     CDS             complement(41758..42087)
                     /locus_tag="PLF66_03450"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HMZ63125.1"
                     /translation="MEYKLVEGIQLIKMDGSILQSDSGRLDRYFSQIFSSESRRVVID
                     LTDANHISSSVLGQIVFLKNKLKALSGDIRLVITDDDLLELFDLTMLNKVFEIFPDVD
                     AAFKSYQ"
     gene            42191..44665
                     /locus_tag="PLF66_03455"
     CDS             42191..44665
                     /locus_tag="PLF66_03455"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HMZ63126.1"
                     /translation="MAVLRILYHSFLNLLSFLNSIKWRVFRLISFVIVFSIIVLCLSA
                     YIVWIKEKPNVKANLDKYRSEVSNYYDSVKTKPIRIYDKNSKLIGEFNRRNFKPIRTD
                     NLQEHANIVWALLSSEDRDFYKHGGINYSAIGRAILVNLTKFRLLQGGSTITQQLSKL
                     TLNLGERNIYNKITEAFCTYYIENQYDKDTILAMYMNQIFLGEGNTGLEEASRYYFNK
                     SATKLTPAESALLVGIIPAPSVYNPIRNLGISLERQKRILNDMAKNINLHMNQKQVEK
                     DFPKKIDESIRQFKNAYKIVEVKVTEKVKYTSDIGKFGIDRDFKLNLAPDFNESIRRY
                     VLEKFTNDELEKTSLNIYTTLDYEKQSIIEASLREGIEDVRKVLEKEKQAYSKKGNDE
                     EAKRESEIIDSMNGSAVSLNPYNGNVEALIGAYKISSIYRLNRAEEIKRQPGSSIKGI
                     IFALALEKKIITPSSIVKDEKINIGGYTPKNWYGSYKGYITARQALAQSVNTVAVKLL
                     QEVGVNYFLEKMSLILSVPVSDLKERFGNNLSLALGSGELSPMELAIVYSTIANGGIR
                     IRPKKIIKITDDDDIDRTPPELYAEEQAEQILDPVACAMAVNLLESVLSPEGTLPARS
                     KDKLPLAGKTGTVQTPKKAAQKWGNRKGIRDSWFAGIFPGNVTTIWVGNDQSAPFPGS
                     GAGISGQVWLRYVSYMKSRIDSGEKLIKPFEGDFVQVDICGEQGILLQDAPECKYPIY
                     AQYYYRGTEPGANSSPPEVKIEPQKANAGAEEGISFEDEPDVNFEYPKEEPKDPEPTI
                     EKVREPEANPTENNVLPEPKKTQTEQ"
     gene            44669..46930
                     /locus_tag="PLF66_03460"
     CDS             44669..46930
                     /locus_tag="PLF66_03460"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HMZ63127.1"
                     /translation="MAFIKNIFQNTGIAFKYSLSLYLICLIFNTSFRVMGFDLSSEIN
                     SILNGDFFVLILLINAKILLIYYIFFSVIQISLDFLINELSKGKDSWIQKRKRSLIYL
                     VFILFTLSLFFHSIIHYPQVYGEFFYIRHTYLQPLLYFLTDYVNPKVFEFFFLGFIAI
                     YLSFLFVGFIYKSDIRYLFYLSFFLLIIFFHVISFVYGIIPIFLSFQIIRQIKTNVNR
                     FLLLPTLFLITLIIGSYYYFTFLFHIPKESSKDFNLLIVSADSLRADRIGAKVNGISI
                     TPNIDKFKEEAFTFEDHHVTIPRTFPSWADLLSGEYSMSHKIRDMFPAPEEKKNLGSP
                     EFPTIGHYLQKKKYKTAVFSNFAGDIFTRADFGFQEVHTPNFNAKILVIQKNLEFQVF
                     LLPILTGSFLRGGNYFEEINGFSNLGDGDRILPDIYSFIRREKSNPFFLNVFFSVTHF
                     PYSPPYPDYKKFTDPKYYGKYKYFKFVDPTSDSKPDEKDIEQIKAIFDASVYSFDESF
                     GKLIKYLKKHSLYEKTLIVITGDHGESLYEDIHGHGHGEHLRGPYITQVPLIIKFPHT
                     FYKERGTHIFKGISSSIDLVPTLIDFFQIDANKNFPGQSLIPILGKENWDEERFVYSE
                     TGIWFSDIGDHFFQSQRIMYPSILKMHRIVPEEEHQIMITDPYFRETIAFAKHRALIT
                     SKYKFIYIPTRDGVIYELFDRVNDPLNQNNIADMNPYIVEQMKRNLYKLAEEKEGANI
                     IGDYILPPPINKD"
     gene            complement(46931..47137)
                     /locus_tag="PLF66_03465"
     CDS             complement(46931..47137)
                     /locus_tag="PLF66_03465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63128.1"
                     /translation="MIRTFYFILTILILTFSIQLIAQKQAPKQEPKKEKAKKMEPKKE
                     GNEEQKERKDNRAMTKEDKEESRR"
     gene            47569..47724
                     /locus_tag="PLF66_03470"
     CDS             47569..47724
                     /locus_tag="PLF66_03470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63129.1"
                     /translation="MLYFANLTSIILFVFGISFVVAEPWTKSERKIVKFEGKSNSKIT
                     LNDHYSK"
     gene            complement(47712..48737)
                     /locus_tag="PLF66_03475"
     CDS             complement(47712..48737)
                     /locus_tag="PLF66_03475"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HMZ63130.1"
                     /translation="MEAPNSEDKEKEIHYLRKIIDAYEKVTDFGNDELLEAYKIISAQ
                     EKVRDLAREELINATANLEELRSRETKITEEIMRILDEDVTNEELILHKLEALSKKSN
                     AGFFADLFYVIANLELAEEDAAKLWQEIYVHRSDMCSKLGRSVSFRVALLDHILENNR
                     VIRNPKIIELNLFDSVVKNSVVDELTTLYNRRYFNLAFKRELKRAKRYNRSICLFVFD
                     VDDFKIFNDKYGHTMGDDVLRLIGFILKESFRQEDILCRCGGEEFAVILPEIDTRSAL
                     VACKRFSESLKLASVKKLQIPVTISGGVSEFPDHGDNPEDLYKAADIALYKVKSNGKD
                     DVISYLE"
     gene            complement(48910..49668)
                     /gene="fabG"
                     /locus_tag="PLF66_03480"
     CDS             complement(48910..49668)
                     /gene="fabG"
                     /locus_tag="PLF66_03480"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017851759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HMZ63131.1"
                     /translation="MIDFKNKTAIITGSARGIGKAIAMKFGGLGANLVIVDMNEEATK
                     ATAEEMKGLGYKAIAVTCNVTKEEDCAAMVDAAVKEFGSVDVLVNNAGITKDTLLMRM
                     KKEQWDAVIAVNLTGTYNATQAVIKTMMKQKSGSIVNLTSVVGIDGNAGQTNYAASKA
                     GVIGFTKACAKELSSRGIRVNAIAPGFIETDMTAAIPDAIREGMKKAIYLGRTGKPDD
                     IAGAAAFLASDAASYITGVVLEVHGGGFKAGSGE"
     gene            complement(49686..50690)
                     /gene="plsX"
                     /locus_tag="PLF66_03485"
     CDS             complement(49686..50690)
                     /gene="plsX"
                     /locus_tag="PLF66_03485"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017215784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HMZ63132.1"
                     /translation="MWVAVDAMSGDFGPAGLVEGSVDAVNQYGYSVVLVGNEEILSEE
                     LLKYEYDTSKIRIVHATEVIGMNDSPSTAVRSMPDSSIVQSVKLVADRECVGMFSPGN
                     TGASMAASLLYLGRITGVHRPPIAVPLPQEEGRPLLLLDGGANVDCKPEYLAQFAVMG
                     EIYAREIFGVAQPKIGILSNGEEDKKGNTLSLKTFDLLKKMPVKFIGNIEGRDLYGSG
                     REVDVVVCDGFVGNIVLKATEGLSKSIFNILKNNIADSSIAKTGALLLKPTLKAMKKR
                     MDYAEYGGAILLGVNGTCIIGHGSSSALAVKNAIRVVIECAKTEVNIRIAENLIKYGI
                     "
     gene            complement(50696..50896)
                     /gene="rpmF"
                     /locus_tag="PLF66_03490"
     CDS             complement(50696..50896)
                     /gene="rpmF"
                     /locus_tag="PLF66_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HMZ63133.1"
                     /translation="MAVPKKRKSKSKVRSHRAHHAIGKPNLVPCSNCGAFILSHRVCP
                     TCGFYNGKLVVAPKIKKSKQEE"
     gene            complement(51005..54823)
                     /locus_tag="PLF66_03495"
     CDS             complement(51005..54823)
                     /locus_tag="PLF66_03495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63134.1"
                     /translation="MAKLTKDELSKIAEILSPLDKDPIKYETLNPMSRVLRKIKGLPE
                     IITEEETKSAVEEEPIDGGIELPEDAISDLSEPPKMKYFDESDVDIDELLNDASSLMA
                     GQPIASKKDKEPEPEQIQETDFDQNSNFEDTTEDPFGSMDTATSEDPFGAINDEAVDT
                     TQEIQDSDPFAFDETPQTTSDSISTNEDDPFAGFGEDETSTPDISSEQIEDPFADAAD
                     AFEKSSAPNAMDTDPFADVVPTNSFDSDDPFADLDTPTATNVTEDDPFAMDDSATIQN
                     DTQPTSDDPFAGFDEDTNTDTNDNDFTSSQPEAADDPFANFDSEPNAADDPFGDLGAD
                     SDSFGDSNDLAGFEDTNSVDTFDTNDSSTTMEPMPMDDYDSSGFESTDDPFADIQPST
                     GIGHDFDAVDSGQDYESSFDDLGAGIGDVGGNTNSSLDVIDEIGPSSETGSYESLDED
                     LDSLAQEEEPEEDLSDEELAVIQQEILKYPPKLKRTVIDVITNEKLHKAEQRELIEII
                     KASQKPEDIAAYLTEKLGYEVELFDKSGLYSERGIPVISTKPIYTKEGELRRRELIKR
                     TVLIAAASILGVIGLISTYKYVIRPFQAARHYKEGIEYIKKAGLAKDKTERTQALLDA
                     KTSFSKGEKIEPNNLEYLNKYGMAYNKIGEYDQAFEKLFGVVEPDFGAEKNGESESWT
                     KRLEVPYIQLSSKTTWDNDKLPLGGRKEVPDNELMVLVSGSPKKQVERKIRRAGAYIV
                     ARLEKNIHDNQTYINLGKFHSNIANLFNKPAITATRNYKNDNLAVNYFKEVFTDGEDE
                     NNVEATAGLAKVYYNQKNFAKSVFYYNKIVEKFPSNAIGHGGLLSNYIEMWKGDQNPQ
                     FVLNHHRQVRNSLNIEDDLSLFVLAKLASFYIDLDASEVRIKYNINPEDQVTNMDLED
                     NVEHLLNLAFFKTEKGEDGEDVKGDEFAEGYYQRGRFLINKGESIRALKQFEFAASYD
                     PAHYLAVMEMAEHYMRVNNYSESIDLLQNAKKRYEAYKDLYGNREEDETLLNGDPGRI
                     FFDEGKIVFMESSLISKDGKISEFPDRKVYPDKSLSQLSEEESNRRRNLNNAMQKFDI
                     ALKLNLKDSKLKRELYYYKGWIEYMRSDYEAALNEWSNLSEEDTYRNSNVMIGRANAF
                     YNLDQLNASLGNYIKLKEDFEEKESLIARPTPDESAHQEIYNILIAVYNNIGAVYERK
                     GNSAQALKHYWKAIEIARKIGLTTEIANYNKDMVFKAKPGGALPLLDDWLSPTIDTVK
                     ELQNTKKAKL"
     gene            55160..56071
                     /locus_tag="PLF66_03500"
     CDS             55160..56071
                     /locus_tag="PLF66_03500"
                     /inference="COORDINATES: protein motif:HMM:NF027875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen-binding domain-containing protein"
                     /protein_id="HMZ63135.1"
                     /translation="MQKTNKKKKITRPKKRFQRKYLILFIILSLFGFSVVGQDTVDWI
                     GSYSSKELEGTEEKASTNKIYFYWQIASLKRAVPPRYIRMIDIDTYAQKGEFLNSGIL
                     FTYNGLKNNEVSICGNFSSWRCIPMTKNRFGVFHIIVPTNFKNRKDEPVLSYEYKFKV
                     DHLFDYDIENSNRHSDGAGSYYSEYTLDTVDFKKQVSAKVIEEEMTEDLDFITVEFQI
                     YKPNASVISLVGNFNQWNPEHDYLQKDRNGIFKLRKKLRPGEYLYNYIVDGEKILDTY
                     NSETRHRIETDELSSYLKVIDNKMSSL"
     gene            complement(56068..56868)
                     /locus_tag="PLF66_03505"
     CDS             complement(56068..56868)
                     /locus_tag="PLF66_03505"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010570479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HMZ63136.1"
                     /translation="MFDSFLDSPSKMLIVWILNFSLLTLRYFIFAGIAYGIFWIWKRD
                     IWQYKRTQMDYPDKSKIIYEVKYSLITMTVFATVGLFIFLMKKNGYTYIYNNISERGT
                     GYFIFSVIIAILIHDTYFYWAHRLMHHKLLFKYVHKVHHNSTNPSPWAAFSFHPTESI
                     LEALIVPMLVFIMPIHPLAILAFLLYMTLMNVLGHLGFELYPKGFTTHWFGKWNNTAT
                     HHNMHHKFFNCNYGLYFNFWDRVMGTNHPKYTDTFEEVKSRLPQVVNN"
     gene            complement(56891..57211)
                     /locus_tag="PLF66_03510"
     CDS             complement(56891..57211)
                     /locus_tag="PLF66_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002622262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HMZ63137.1"
                     /translation="MSQFYEKHIFVCENIREGENARVSCGKSNAKKIREYLKIKIKEV
                     APNSKIRVNMSGCLDRCELGPIQVCYPAGDWFALKTEKDVDDFIKNYIQNENRDGISH
                     LTVI"
     gene            57272..59119
                     /locus_tag="PLF66_03515"
     CDS             57272..59119
                     /locus_tag="PLF66_03515"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HMZ63138.1"
                     /translation="MESLILNQFKKLEDQHKSNIFNKIYESNFTNLYAGLLALCAIHI
                     PIIILDYLKLKKGMWEINLGYKYIFFMHLGLYGFLFFSLSFYLFLKKYNYSFKTKNVF
                     CFVVSLLLQLWSALFSSIDQMIFGDISIFYISIFCLPVFFKFRAELEFFKLALAEIAF
                     ILLIFFFQSIPDKAEGIIVNSGVAIVLAFVASRVSFSFKLRELASHAIILQQKEELYS
                     EKEKSKEAARNKNEFITSLSHELQNQMRGLIGILKLLDETKLNHEQKDYTNLLDSSVK
                     SLLSFISDIFEFTQIESGKTELENNIFYLPDFIKKIISISSSQASLNDNIIKYYIEDN
                     VPKYISGDKIRLSHIILNLLSNAIKFTKSGTIELIVSKVRDNKNLCLLEFQIKDTGSG
                     IEESRLDKIFDLITKEELSNLKKFRPTGLSLAISKKLITMMGGNIAVESVRGAGSNFH
                     FTFPTENHDDSQKKEDTIIEKGTLKEETIRDSDLSRKMNLKILLVDDNEINLKLGKKV
                     FEKLKYEVSTASDGKEAVELCNSKQFDIVFMDLYMPVLNGFEATEVINKSKKDIKDKP
                     LIIALTANATKANKEACMKLGMVDFISKPLDVSRIKKYLIFLAEKFKLD"
     gene            complement(59122..59757)
                     /gene="tmpT"
                     /locus_tag="PLF66_03520"
     CDS             complement(59122..59757)
                     /gene="tmpT"
                     /locus_tag="PLF66_03520"
                     /EC_number="2.1.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016545618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008119 - thiopurine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiopurine S-methyltransferase"
                     /protein_id="HMZ63139.1"
                     /translation="MEKEFWLKRWQDRNIAFHQEEVNLFLVQYFKDLGIQKGDRIFVP
                     LCGKTLDIAWLLAEDFQVVGVELSEQAIKELFSELNENPTITHQKKLIQYKIENLTVF
                     VGDFFDLDQNILGKVDGIYDRAALVALPDSIRIRYVEHLQNITNCSPQLVITYEYDQT
                     LLAGPPFSISDKEINKYYRSNYKIEHIIKSEVSGGFKGQVAFENVWKLRRL"
     gene            complement(59859..60632)
                     /locus_tag="PLF66_03525"
     CDS             complement(59859..60632)
                     /locus_tag="PLF66_03525"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HMZ63140.1"
                     /translation="MLKLEKVSFIRNDRFILKDIDLSMESGQNWVILGKNGSGKTTLI
                     NLLYGYLWPTSGKVSVFEKTYGEYPIKLIQSKIGILQSSHQEERLQRNLSVKDVISSG
                     LLNSIGIYSELTQSQLEKIDQLIESNTWIKDSTQNYSLLSSGEKKKVLLLRALISEPE
                     ILVLDEPCSSLDLAAREDFFSLLESYRRYAKLIILITHRTDEIPIYFNYALLLKEGQI
                     VASGRKNEIFNSNNLTEVYGVNVELIEKDGIFFTNIVKH"
     gene            complement(60634..62106)
                     /locus_tag="PLF66_03530"
     CDS             complement(60634..62106)
                     /locus_tag="PLF66_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016757908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HMZ63141.1"
                     /translation="MLLDINDIVSKLIKGQPVSVRFKYTNDKILQEFHVLFIHILAYF
                     DQLFLLEVSFTVLKEILYNASKANAKRLYFIRESLDISNPDDYQKGMAVFAEEVTMKW
                     NEQQDFLDKSEFYIQFEAKIKDNTLYIKAENNAPVLPEEQQRIHKRIEAAKKYNDLSD
                     AFMDMSDSTESAGLGLILTQILLKNSGIGRENFNIEFQSDKTIAHFTIPGKVIPIVTN
                     SKFNEQILNEIDGLPPLPQSVSKIINLCNKPDTDINILTAEIEKDAVLSADLLKLSNS
                     SLFITRNKANTVLSAVKVVGLKNIKNMLYVSGVRKIMGNRYDKVQKIWDHCAKCSFFA
                     KVIAVDNSKHKISDLAATGGLLHDIGKLIILSLDKKVVEKMTNLQIQEKDSSVLIEEF
                     TFGISHADIGARLLKKWSFPDDLVHIVEFHHRPFLAPAEHRDLLEIVYLANMMLDALD
                     GRANFFTINQEILKNFKLDNEAVFTKYLEKIDTQFKTSHA"
     gene            62251..62481
                     /locus_tag="PLF66_03535"
     CDS             62251..62481
                     /locus_tag="PLF66_03535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63142.1"
                     /translation="MINKVAKQGYKSFEDTPELQEDNFQNLESMVESLLSSMNQKKSD
                     KKKKQTTPKNWKKSKKKKRSEEIGIIELPFTF"
     gene            complement(62571..64196)
                     /locus_tag="PLF66_03540"
     CDS             complement(62571..64196)
                     /locus_tag="PLF66_03540"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HMZ63143.1"
                     /translation="MKKSLSIFILITIAIIAIRSMRFLYQSLDWAIEYCVDMVLMKFF
                     AWSLDKGSWPYTDVLTYNLPMTIYINWFALKIFGNSSFGFRLFDVTWLILFSGTTFVY
                     LNRKIPTLFAFFGATLCLTLEENATNFGALQRETMMLPFWILSLISYDKIREGKSRIK
                     HGFLIGIYILITVLIKPTAIILLGCILLSLFIIDLKERKVSIKENLTFYISIFLGSLI
                     ILFMTLLPFLYKGIMFVAFDQWLSYFRDLSLSLEIISPSQLLLNIFTFSFSHWFIPLN
                     EPIFEFQNNGHLSLFHLSIILFYLFLLFRGKVDIGPLFILITGLLNYLIQAKGFAYHL
                     FPMWFACVLIVTFLINYLFQIALNNESGKKIIASFVIFLVCITLIFQQARSLRLYKGT
                     NLYYEWNQSKKDFPKTETLEAIKSLNQSVKNRPTKIQIFEAYHSVSLSAIMNQDMILA
                     SRYPEAYVFYNEHPAMDKYKKDMMQSLQKNKPDIIVLNREGTFRVKKDLFQTFPELGD
                     FLKNYTLEKEIKEINNVNYDLYVLNESGKTEKK"
     gene            64277..65815
                     /locus_tag="PLF66_03545"
     CDS             64277..65815
                     /locus_tag="PLF66_03545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63144.1"
                     /translation="MKNIETMSPRIYVSFYFSLMIHLSLILLFFFPFSHSLELFCGID
                     EVMIIPEEKNSWETMLSIDFSLEDGDSKNEDKGDAGSIVSNQKPKDNESSDEKEGTNS
                     DFSTESLEKKGWKDLVQRLKDTKGLRANFKEDFNNVIKDGTVADSYIHRERFYEDIIV
                     KEVYPTVHSIEKPFREDIKNAPEDLSKHEERNDIIDKFRNEIVDEPDTIIASINKKSD
                     KVTKSPLKMSEQERNKYFDNSLTLPKEEQLSDFIERYLGYDPDNGDLPLMFRELYYKN
                     LQRLAYTFSSDSSYFSIDYLQENLNKEDYLKNAMNLVSELKGTKTATEILFTLENIYE
                     IQGNALQHFFISQENLKNLTPEQKKLIRFETLRQFVNRYAPVIKDKKFNNHQDALALY
                     FKKKLEIMDYILTTTPNHYRKSDALFEKGRIYWDLGMRTNNIKERKEAVKVWSQIDVT
                     PNTGDFLNKDFFAQISPFIQEWKVSNSAQSQLQIDSLLAGRLIPSLMDKKKREEQLLW
                     KKKN"
     gene            complement(65812..66123)
                     /locus_tag="PLF66_03550"
     CDS             complement(65812..66123)
                     /locus_tag="PLF66_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63145.1"
                     /translation="MKKKIFILVLLIWFPISAGNVDSQCTFNGIKLYGKIQIVNSFPD
                     IKVQVVESFPDLKVQMVNSFPSSCGKWQAVNSFPDFKIQFVNSFPDIKIKYESSFPGL
                     P"
     gene            66216..66725
                     /locus_tag="PLF66_03555"
     CDS             66216..66725
                     /locus_tag="PLF66_03555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63146.1"
                     /translation="MLVSYLFNFGLFSQAASDNYVEVFKGQRVQQGELFYNKALDFYK
                     DSNYKACIDKLNEFLFLFPGHPATLKSLKLLSLTYKKRDLIDESIRTDLLIYKENPTT
                     EDGLTSYLDAGKKLLMIGRAKEGKNILENIKNQLYSSKIAKDAEIELKQNKILEENGF
                     QIKEEMSKD"
     gene            66848..68071
                     /locus_tag="PLF66_03560"
     CDS             66848..68071
                     /locus_tag="PLF66_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003006165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HMZ63147.1"
                     /translation="MSAPSFQIVNYTANSYIIVEGKKEAYNFYIVREGKVSVGRENPV
                     VGEDPNQVVGPGDFFGVVAAMSQHPQIESARALTNVSLISVSYDQFGTLIQRNTPVAM
                     KIIRYFSMKLRQFDQTITRLSFRNTVSEDLNQLYNMGEYYFSQGNIEHAVYAYQSYLK
                     YLPTGQFVPQVKKKLMSLNQPLALPPVDETKFNRSYADNKVIFCEHEPGRELYIIQSG
                     KVKISKIVNNNEVMLAVLQTGDIFGEMAILDNKPRSASAVAFGYTDVLAINKANFESM
                     VKAQPQLASRLITILSERIWIAYKQLANLMIKDPHGRIADTLLTLAEKNRAKIAPKSF
                     YDFDITDRDLLKMVGLSDNRDEKILYSLFEANKFLKIDQGRVKCYDLAELEKLVQYYR
                     KKTIMDSKIAKEKIR"
     gene            68084..69637
                     /locus_tag="PLF66_03565"
     CDS             68084..69637
                     /locus_tag="PLF66_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000249631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HMZ63148.1"
                     /translation="MKNLAAIDLGTNSFHIIIVKVNPNGTFESLGKEKESVRLGSGAG
                     PNEVITPDAIERGIKCLKRFKGLAEIQNAEIRSVATSALREAKNREVFVEKARKELNL
                     NIEVISGFEEARLIYFGILQGLPVFDKKIMLVDIGGGSTEILVGQRGNIEFAQSLKIG
                     AIRLTDKFFAGDPDNESDINQCKLYVEGMIAPYKREIEKLAPEIIIGSSGTIQAIAQM
                     VLAEENEEMPRSINNFSFTNDQLAKVRSILNAANTLKKRTKVPGLDVKRADIIVAGSI
                     ILEQIFKTFNIKSMTVSEYALREGIIYDTIKKREERDHKQDSESALDNIRLKAVNTLF
                     TSFPYEADHVRKVTALSLKIFDDLKSLHKCTEDEREYLEAASLLHEIGFGISHSSHHK
                     HSYYIIRNSELMVGFNFDEIEIIALIARYHRKSPPKPKHIEFNKLNTNNQLIVKKLSA
                     ILRIADALDRSHQGIAEEVTCELQNEVVVFKVKAKKDVDPHIDIWSAHQKKDLFEEIY
                     GKKCAFVVI"
     gene            complement(69638..70387)
                     /locus_tag="PLF66_03570"
     CDS             complement(69638..70387)
                     /locus_tag="PLF66_03570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63149.1"
                     /translation="MIIAIDPESEYLDNTESLLSGAKYNFVKANEFKKGMDYVKTATP
                     DLLMIGIKKIDKELIDTIQFFKKNSNTKNIPILGIYKEQKRSDIENGFKLGINAYLVQ
                     PLDKHRLLDKVKELMEISKENQSDQIMTRKNHILIENPTPGLTKITFKSGIYKYVLPQ
                     VKKTFNMEFLRTVELNQCCIDIRDIPEMTMDEVSMFEKIVGLFGSKKISIIAGKHLGK
                     IIAGSELSDKANLFMSLEDYVVFLKAAKILS"
     gene            complement(70553..71677)
                     /locus_tag="PLF66_03575"
     CDS             complement(70553..71677)
                     /locus_tag="PLF66_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000812250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63150.1"
                     /translation="MKNPKPSTADEIRFYSITIFTALLILFGYFYGVSHYYLGWMAFA
                     VAAFSVAGNDAIQTVGTFIESKRKVHWFPKVAMLGGLLIAVHLYGWMRYDGEIHFKRL
                     NTFSEVNQFNILQLMAPIVLVIITRLKAPISTTFLILGLFGGNSNIEKMLNKSFSCYI
                     IAFLFAMAFWGIHSYYNREEYLIEFEPELAKEKKWASLQWASTAFLWIAWLLQDTANI
                     AIYLPRKLSLSEFLFAVMIICFAITIIIRSNGGTIQEVVSEKSDIAYSKAATLVDFAY
                     GIVLFTFQLQSTIPMSTTWVFLGLLAGREIILHLVTREDRPYLQTFRQVSKDVLLATL
                     GITVSILIYGASSYYYPDKSAKLNILRENEKTNSNRIYSK"
     gene            complement(71777..71858)
                     /locus_tag="PLF66_03580"
     tRNA            complement(71777..71858)
                     /locus_tag="PLF66_03580"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(71822..71824),aa:Leu,seq:cag)
BASE COUNT        24249 a        12841 c        11049 g        23720 t
ORIGIN      
        1 ggaacagagg aagaaggtgg aagcggactt agtttaatca tcgcaaaaga cttagcagaa
       61 aaaatccatg gggaaataaa actcattccc aaagaagttg gcttaattgg tgaagtgaat
      121 attatagcta ataaaaagga atagctaata acgttttcta gtaattgtat ctcattaaaa
      181 ctatcaaact gtattgattt tatatcagtt gtcaaaagga tttgtatatt tttttatgga
      241 aaattaaaat gataatccgc tacttactaa tttttatcac tcaatccatt ctgtggattc
      301 tatttttgtc cttttcaata tttgcagaaa ccaataaacc ggattcaata aaaaccgaaa
      361 ctgcaaaagg gaaatggggt tatatcttat cttcctcctt accaaaattt cctattgagt
      421 atttagaaac catttccaaa gagtatgaga ttctttgtat aaatggttta gaattaaatg
      481 ggaaaggaca gcttcgttat acttctcagt ttattgataa aattggaaaa tctaaattaa
      541 agacaaagga tcagaataga gcaattctct atccaatgat tacagtttcc tctatacgag
      601 aagggataga actcttaaat agcgataaat ctaaacgtaa ggcgacagat caaatattag
      661 aatttctaat tgaaaataaa tttccaggtg ttcatttgga tattgaatat ctaccatctg
      721 aatttgattt aaaactttcg gatttcttag cgttattaaa aaaaaatcta aacgataaca
      781 atttgcgatt aagttttgca gtttttccac aaattgattt tccatcttct ataagtaaat
      841 tacataatgt ggaaaagcta gcaaatgatg ttgacgaaat tgttttaatg agttacgacc
      901 gacacaataa aaagacggaa gctggttgcg taacgtccct tgactggagt aaaaaaaaca
      961 tcactcattt actaaaattt tttaaaccag agcaactatg gctgggaatg ccagcttatg
     1021 gatatgaatg gatcattgaa acaaaaaaag taaatatcgt atcctcacga gatggcgaac
     1081 tatacaataa aacttgggaa ggaaaaagag agtcatcagg ttgtttaaaa attctaaaag
     1141 aagataaaaa gaaaaccagt ttaatctatt ttgcggatga ggaaactaga aataaaatga
     1201 gagaagtagc ggaagagttt aaaatccgag gcaccgcgat ctggcgactc ggattggagg
     1261 ataattagaa ttaacccata tttaaatcag gtgaaagttc aaaatttgaa ttcacatttt
     1321 gaacatcatc atgcgcttca aaagcatcta atattttcat aactttttct gcgacgagtg
     1381 gatcagatat ttcaactgta acagatggaa caaattttac ttccgactcg attacttgta
     1441 aaccttttga gttaattgct tcttggattt tctcgtaagc ttctggttgt gcagtaattt
     1501 gaaaaacatc ttcctgtaaa acaacatcat cggcaccagc atccactact atatcaacca
     1561 acgcatcttc agatatttga tcagctgaaa taattataat cgctttcttc tcaaaaagac
     1621 gactaacggc tcccgcgttt gctaaagaac cgccaaactt agttaaaata tttttaattt
     1681 ctggagtagt tctagatttt ttatctgtag tgacagatac catgattgca ataccgccgg
     1741 gagcgtagca ttcataaaga cagtcctcat acgttacacc ctctaactcg cctgctcctt
     1801 ttttaattgc gcgttcaata ttatccttag gcatattggc agattttgcc ttcaatactg
     1861 ccactctaag tctaggattt gcttctggat cagaaccacc cattctagcc gcaacggtta
     1921 tttcctttgc atatcttgta aaaaccgcgc ctcttttggc atcaattgct tcttttttcc
     1981 ttttgatggt cgcccacttc gaatgtcctg acatataccc tctctgaaac tatttctact
     2041 tacatcgttt tctgaatcca tactttttga aaataatttt ttctattgaa atgcctagat
     2101 atcctttgct tttaatctat ttgacagtgg tggaatacaa tatttcttaa gagagatatc
     2161 taaactattg tgctgaaatc gaagaatcga tagtaaaaat ttaaaaatag agtcctcctt
     2221 tgaaatggct tattggcaag gaagattgcg agtgcaagga ttcaaaaaac aaagaagcaa
     2281 aacagttcaa ccggctttcc ttccaaacct agcaacaaaa tttaatccat ttgttgtgtc
     2341 caactggttg tttagaaatc gatatatgta tgctttggct aatggatgtt tgcctctagg
     2401 agaaattata tgaagtggat ttatctaaca atcgcgatca ttggggaagt aattgcaact
     2461 tctgccttaa aatcatcaga aggtattaca aaaccaattc cttccacaat tgtaataatc
     2521 ggatacggtt tggcttttta ttctttgtca ataacgcttc aatccatttc ggttggtgtt
     2581 gcttatgcaa tctgggcggg agttggaacc ttactcatta cactcgttgg atattttttc
     2641 tttcagcaaa agttagatat gccagcagtt ttcggactta ctttgatagt agccggagta
     2701 attgttataa atttattttc aaaaagtatt tctcattgaa ttagattatg tataaaaaca
     2761 tagccactcg attttaagat aaataaaatt ctattttttg gctaagtttt tatagaacca
     2821 aagaaatatt gactttctaa acaaattgtt taaagaatct taaatttcct gaatgaatat
     2881 agcggatatc attaatcgca taacgcatca tcacaagacg atctaatcca agtccaaacg
     2941 cgaagcctct ccaaactttt ggatcaagac ctgcaaattc aattacattt ggatgcacga
     3001 gtccacaagg aagtaattcc acccagccgg agtgtttaca aacagaacaa ccagttcctc
     3061 cacaaaccat acaagaaata tccaattcaa atcctggttc tacaaaaggg aaatatccag
     3121 gtctaagtct gacttttact tcttttttga aaatttcagt caaaagaaca ttcattgtat
     3181 agattagatt tgcagtggag acatccttat caattaccat accttcaatt tgatagaaag
     3241 tattttcatg agacgcatcc acttcttcat atctaaaaac tcttccaggt gcgataattc
     3301 taaaaggtgg tttcaaattt ctaagcgctc taacttgaat tgcagaagta tgagttctta
     3361 ataaatttcc atctttagta taaaaagtgt cttgcatatc tctagcagga tgatctgaag
     3421 taaaatttaa cgcttcgaaa ttattatagt ccgtttcaac ttcaggtcca tccataattt
     3481 gaaaacccat agaagtaaaa atatcttcta cttcatattg aatacgactg agtggatgaa
     3541 gtccgccaag atctctagat tgtagagggc gtaacgtatc aaacttctca tttttcaatt
     3601 cattttcgta gaaatccagt ttaatttttt gttttagttt atttacaaga tcttcaattt
     3661 cttttgatac ttcgtttgct ctcgcgccta ttgtcttctt ttcttctgga ctcaaggcac
     3721 ctagtccttt cattactcca gttagttttc cttttttacc tacaaataga tttttatttg
     3781 tgtctaggtc tatttcggtt ttagaagtcg gcaaaacatc gtttgcttct tttagaatgg
     3841 catttagatc atctaataaa ctcattattt ttttcctttt ttctttgact ttcctgtttt
     3901 ttctttgaaa gactcttttt gcatttctct aactctatca gtaacaattt caattatctt
     3961 tggataaatt ccaccaaatg atccgttaga catagctaag attacaaatt ttgactcata
     4021 gttttctaac tcatcttcca attttaaaag caattcattt gtatcttttg cataaattgc
     4081 ttttttaatc tttgatttct ttggaacttc tttaactagt ttttttacat ctaatcgtat
     4141 tttcttatct actttattta cattgaatac ttctgtaata atagcaacat ttgatttttt
     4201 aaaactctct gcaaattcct tttgaaaaat atttcgatga gaggttgcgc ttctaggttc
     4261 aaataggctt ataataccat gttccggata atttgtctta accgctttga ttgtttcttc
     4321 tatcgcaaca ggatgatgtg caaagtcttc cattacaata gcgctttctg tctcaaataa
     4381 tatttcttgt cttcttttga ctccaggaaa actttcaatg cctctaaata tttcttcctc
     4441 tgtaagccca atcgccttac aaagattagc cgtaacttct gtatttctat aattatgcat
     4501 cccaatcaaa ggactcttta attcacgtcc agttttagcg gaaaacgctt tttcttcttt
     4561 gacatgattt ctagttaaga accctattct taaaattgaa tctttctttc caacttcata
     4621 tttttgcact ggtgcgtgtt taaaattttc acagatttct gttaagttct ttgatccttc
     4681 ccaatagaaa actctcccaa tggaaggaac aagatttagt aaacgtttaa acataactcg
     4741 aattgcatcc agatttggga aaatatctgc atgatcaaaa tccaatgagg ttaagattaa
     4801 ataatacggt ctataatgta agaatttgga agatttatca aagaaagcag aatcatattc
     4861 gtctccttcg attacaaaat attccccacc tcccaattca aatccagcat taccatcttt
     4921 acgaattcca ccagcaaata gtcctggttt aagacctgct tgtttcaaaa tatgatgaat
     4981 caaaaaagta gtcgttgttt ttccatgtgt tcctgcgact acgattactt tcttcttttt
     5041 tagaaagaaa tgacctatcg cttgcggcat actcatatac tctatgcctt catttaaaac
     5101 atattctact tctggatttc cccgtgatat tgcatttcca attatgacta aatcaggctt
     5161 accaacatta tccgcagaaa atccctcaaa tgttttgagt ccccattcct ctaatttatc
     5221 agacatagga ggataaagtt tttgatctga tccaaaaaca ttatgccctg attgttttaa
     5281 catgaaagca agattaccca tggcaatccc acctattcca atcatataga tattcatttc
     5341 atttcctaat taaattaaaa atagttagag caactgcgga taacgtcata cataaaatta
     5401 aaatgtatga tacaaagact ataaactgct taatcgtaaa accaaagaac atctctaaag
     5461 atgtcttact taaataaaga caataaataa aagcagataa aattaggaaa aaattaaaag
     5521 gctttgggaa aatccaaaag actgctgatg cactaaatgg aagaaaagaa atcaaaagta
     5581 aatctctttc ttcaatcaca tcaatggaag cttttccagt tctaagtctt aaaagtaaaa
     5641 tatcaaaatg atataccaaa aataaaacaa gtggataaac agcaatagcc gttgtaattc
     5701 catctgtcag tttaggagga gcctcccacc caaaatgaac agaaccaaca aaagtaaaaa
     5761 ataaattatg caataattta aaaacagggg ctaaaacaaa taaaaagaga tgaatcctaa
     5821 aaacactgaa tctagaaaaa tttacatttt ggatataact agaaaatgca tcttttggag
     5881 atgcaaagat atcagataag agttgaattt cttttttatc aatggattct tgtgacattt
     5941 gacttggttg gttatatttc tttccagaat tttaccaagt gtcaaatttt caacttagaa
     6001 ttgcatcttc tagtaattta ttaatggctg tattaaaggc atcttctagt ccgtctgccg
     6061 gttttatctc tacacaaaaa tttggatctt caaatggatg ttcatatact aaattgttaa
     6121 aaaatttttt cccatccagt ggacttgcat attctttgta tatatcaaga gaaacaaaac
     6181 gaaatccgtt tttctcaaat ttcatcgctg gacgaaatgc atctacatct gtgatcctac
     6241 agtcatcact tgcatgtgca atcgcccaaa caaatcgtat ccctctacaa agcaatatta
     6301 gattgccttt tttaattttc tctaatgttt cccactttga aagatcgctt aagtctttat
     6361 aaactctatg ggtagaacta ggagccgaaa aggcttttct ttttataata ttttcgaaag
     6421 tattgtaaag gtttactcta aatatactca tacataaatt cctacgttaa tttaatttcc
     6481 tgtctctttc tgacaagttt ttaaaaattt ctaatgctcc aaatcttacg agttactcat
     6541 ctcgcacttt taatattctt cttttctttt ctttacaagt aataaataca aagaaggaag
     6601 aactgttaaa ataaaaatca atgcagaggt tagtcctcca acgatcactg ttgcaagtgg
     6661 tctttgcacg tcagaaccaa cacccgatgc caatgtagcg ggtatcaatc caaggagagc
     6721 aagtagcatt gtcataagca ttggtctcag ctgtataata gccgctcttc ccaccgcttc
     6781 ctcaatcgtt atatttgcat tgtctcgcat aatatgattt gtccgcgata caaacaaaac
     6841 acccgccatt gttgcgattc caaagagaga aataaatcca accccagcag aaacgttgaa
     6901 ataatagcct cgaatcagaa gtgcaatgat tcccccaatc aaagaaagag gaatacaagc
     6961 cagagcaacg gatacatatc gcaagtttct atacataata aataaaactc cgaatatgat
     7021 agcaattgtt actggaatta caaccgcaag tttctttcca actcgattta agttttcgta
     7081 ttgtccacca taagcaattt tatatccatc tgggagcttt atatgtgcat ccactttctt
     7141 tctcagttca gttacaaatc caccttgatc ccttccccga acgttagttc tcacagaaac
     7201 gtttcgtctt ccttcctgtc taaagatcat tgtaggtcca tcaattaact taatctcagc
     7261 caattgagac aatggaactc tctgcccact tggagatata acaggcacat tttccaatgc
     7321 ttttactgta gaacgatatt cagaagaaaa cctaactaca atcccaaatc gccaaggtcc
     7381 ttcataaaga gtgctaattc tttgtgctcc aatagcagcc tcaatcattt gttgaatttt
     7441 acttacatta atcccgtatc tggctgctga ttctcgattg attttcacag agagctgtgt
     7501 agagtttcct tcctgttcaa ttccatactc actcgctccc tccatatctt ttacgattgc
     7561 aagaatatca ttcgctattt ttcgcatgac gtctagatca gttccagaca cgaagacagc
     7621 tagatctgca atagttcctg taatcgcttc cgaaaggtta tccataatcg gttgcgagaa
     7681 actaaatcga acccctggac aattgatctc taaatcattt ttcattcgaa tcaaaagctc
     7741 ttgcttcgta atcttttccg tccaagtcga gtaatctttc aaaccaataa gaatttccaa
     7801 acgatttggt ccaagtgggt ctgttccatc atcatttctt cccaattgtg taatgattac
     7861 gtttacttgt tcatttttac ttatcacctc acgcattttt gggattactt ttcttgcttc
     7921 ctgtaagtga attcccactg gaaaataggc tcgaatattg aatccacctt catctaactc
     7981 aggcaagaac tcacttccga ttttccaaac tccaattcct acaattaaga caacaatgct
     8041 aaaggcaaca gtcactgttt gttttgatct tcgaatcaat tctacaatca gatttgaata
     8101 tttatgctca atccagtggt aaacaggatt atgccactcg atcggtcctg ggtttgcaga
     8161 ctcaaagtat tttttataca tcattgtcat catcacggga atgaccgtta gggaaaatat
     8221 catagaacca agaattgcat aggcaattgt aaatgccatt ggcttaaaaa gtcttccttc
     8281 aattctctca aaagagaaaa ttggtaaata ggctaaaata ataatggtaa tagaaaagaa
     8341 aatctcagaa ccaacttccg cagaagcact tgctgtaaag gggttaatac cgatttgctt
     8401 ctcttctaca tccgcatctc tataacgccg catgatattc tcaaccatga caacagctcc
     8461 atccacaata atcccaaaat caattgcacc cagggatagt agactcgcag gaattcctgt
     8521 taaatccatc atgataaaag caaataacat cgatacagga atcgttgtaa ccactactag
     8581 ggacgctcga atgctcccaa taaagaagat caatactagt gtaacaattg ccaccccttc
     8641 gatcaacgtt ttagtaatcg ttccgattgt atatttaact aaatcactac gatcatatgt
     8701 atttctaagt ttcactccag gaggaagata attttcattg atctctttta cctttgctct
     8761 aatcttctct cccatctcat ttgaatcacc ccaacgacgc attgcaatga gtccttgaac
     8821 actcgaatct gattctactc ttggagatcc atcttcttgg ttttggaaca catatcctag
     8881 cacaccagtt gggagagaag gaaaaatttc tacagtagca atatctctta caaagattgg
     8941 aaccccatcg tgttgtttta ctacaatgtt ttcaatatct ttaatctctc gaatcgcacc
     9001 taacgatcga attgcaaacc cttgtccacc ttgtcttaat atatttccac ctgtgttcaa
     9061 gttgttatct tggattgctt gtattacatc tcctacttcc aactggtagc gaatgagctt
     9121 atctggattt gtaactacat ggtattgttt aagtaatcct ccaaaagtta caacgtccgc
     9181 aatccctgga atctgtaaaa gcttgggcat tacaatccaa tcttgaatgg ttcttaactc
     9241 cattgctgta tgattttcag aagactctag aacataacgg taaatttcgc ccactggaga
     9301 actcattgga gctagttgag gagttacatc tgccggaata tctgcctctt tcactttctc
     9361 taaaagccta gtcctagcaa agtaatcatc agttccatct tcaaatacga actgaaacac
     9421 cacaagtcca ttgatcgttc tagaacgtct aacgattact tttggaatcg catttaaaat
     9481 tctttcaatt ggaagagtta ctctctcttc tacttcaacg gctgccctac caggaaattt
     9541 tgcgatgagc cttacttgag tatccgctat atccgaatat gcctcttttc gtaaatctgt
     9601 ccaagcccaa attccaagga tgactgtcat gatccctaca agtaatgtag gaatcctatg
     9661 ttgaatagca atgccaataa atcgaatgat catatataat tattcctttt taaaagaacg
     9721 ttagttccta ccaaaatcaa aaagataacc catttgtaag agaagttgtg gattgcgata
     9781 atctcctctt ccataaccca taccaacttg tagaaaaaac ccaccaacca agatatcata
     9841 ggtcagacct acatactgtt gatgaaaatt tgcatactgc ctagttctgt attgatattc
     9901 caaagcagtt gctaaagtag gattttgttc tgcgatatag cttctaatca atgtatcacc
     9961 aaaggttgga ggttgatgat tgtatcctgg gataccatct cgatcgtatt tattctgacc
    10021 accttgttgg gtttggggat caaagggtct gacgttcatt tctccaaata caagtgaaac
    10081 actatgagct atgactgagg tcttccagaa agagtaacct atgtctcctt gaattccatt
    10141 ccagtgttgt gaataacgca ttccagatgc acgcaaaaca aaattggtaa aataataacc
    10201 tacattgaaa ttggcacttg ctggagaacc aatcgttccc ccaaccaccc agaaattact
    10261 atatttttca ttattatctc gaattgattt tttattattt gtttccgggt cataaccagt
    10321 taaaggttct ttctctttat ctgatcttac cttttcaatt gacttttcat tctcttgcat
    10381 gatttccaaa tttgattttt ctttcttttc ttcccgcgca ggctcagttg cttgcgagaa
    10441 aatagaattt gtgaagaaaa acaatattaa acttattgcg aaacttaaaa aataatttgg
    10501 tagagaaaac cggatgcgaa cttttctaat gaacctattc cataatgttt ctaatttatt
    10561 ttgtccttca aagtgcacat ttaaaaacgg ttgcctagta taaaaatcca caaacacgcc
    10621 tttcggtgac catcggtgtc catcggtggt attcttgaaa ttgttttcct tcatattaat
    10681 acccaaaact caggcctttt aaaagcattg ccccaccgat gacaacttct tccccgggaa
    10741 ataaaccttg aacaacactt acattatctg atccagaatt tcctaaaaca actggtcttc
    10801 gaataaactc acctggagtt gtctgaacaa acacataggc ttgtccttct acagtaacaa
    10861 cagaagagaa tggaatcact gcgatatctt ttgtttcatc tccaaactca actttagcaa
    10921 acatacctgg tttaaacttt ccttctttgt taggaagagc cactctcacc ttaacagttc
    10981 gagtcaatgg atcaacgtta tcacctatcg cttccgcttt tccttttaga gtcaaatccg
    11041 gaaatgatgc aaagtgaacc ttgacttctt taccctttcg aacaacgctt aaatttgctt
    11101 ctggaatatc acaaattaac caaacaagat tagatccaac aaattgtaat tcgcttggat
    11161 taaaaccaat ggctctgagt ttcccttcaa actcagcggt ttctgcttgc gcactattga
    11221 ccgcagcctc tgcttcaatg atatctttct gagtagccac ttgattttta aacatatcct
    11281 gcactcgatt taagttttta gaagcacgag ttagggaagt tctactaacc tggtaggatg
    11341 catagattgc attgatatca ggagattcaa agagcacaat tctcccaccc gagacggaat
    11401 ttgtaatact tgcaatgact ctggaggggg cagaaataga aatgaaacca tctccttctc
    11461 caaattttga aaccttaatt tgagaaagac caggactctc tggatcgaat ttgatctttt
    11521 gtccttcctc cgaaactaca ggtcgataat ttttttgtgg ctctacttgt tttttcttac
    11581 atgcgaaaac gaatgggatt aggattaaat ttataaatat aaataagttt ttaaatttca
    11641 ttttagattt tcctttattc attaggttga atatttaata tgctttttcc aacagagtag
    11701 tttaaatttt cgatagaatc aactcgatcg atttgtaatt ttaacatctg ctgaacgcta
    11761 gttcgatagg tttcaaaaaa gtctgcaaac tcaatcactg tcaaataacg cttttcatag
    11821 tttgatatca tcagttgcgc taaatttgta taatcttctg ttagccgatt cttaaaggct
    11881 tggtagagtc tgtctttttc aagaagtcga ttgtatataa tagttacttc gttttcagct
    11941 tgtagctttt gattttgtaa ctcagatctt cttgtgagga tagctttttc tgctgcctca
    12001 atattacctt gatttctatc aaaaatcgga accgttagtt gcgctgtcac tccccaataa
    12061 tttggatagg ctgttccatt tctattatac ataggaccga aggatagatc tggaactgca
    12121 tttgcttgtt gcaaagcaag atttgcttcc tctaacttta aagcttggat ggcaattttt
    12181 agatcgggcc tgttttcaaa tgcatcattg attactttat ctagttttag atctgtaagt
    12241 cttacttcct ctaatccctt agaatcaatt tttggaataa aggttacatt cataagttct
    12301 ggactattta gcaagatccg aagtgatgct tccttttgct ttacttgcac aacagcttcc
    12361 gttctttcat tttccagaaa aaacaagaga gcttttagac gaagcatttc agattgtaga
    12421 acggctcttt tttcgtaagc tttctctaaa gaactaacgg tctttgtaag agcagctatg
    12481 cttccatcat aaaactgaat cgattgtctt aaataatgaa tcccaaaaaa actacttcta
    12541 agttcgaatt taagtgctcg caatagatca aagaattgct gttcgctgat tgctttattg
    12601 atctcagcca cttttgttct tttctcaatt ttgccaccaa gcaaaaagag ctgctgaatt
    12661 tggagagtag tttgaccatt tctagttgta tcaaaatatc tttgtgtatt atcattaaaa
    12721 atattttgat caacagagat atttggattc gcatataact tggcttgaat tatacccgct
    12781 tttttaacat ctatctcaaa cttagcagca agtaaacgca ggttattttt tagaaatgtt
    12841 tcttctgctt ctttcaaagt aagagagtag tttttcgtag attcttggct caaaagaatt
    12901 tgcgaaaatg aaaagaataa aaagcttaaa gataaaaaag catttttatt ttttatttta
    12961 tagaatatcc acatgaatcg attagctcct tcagatcact gctattttta ttttaattta
    13021 aaaccaattc cttagttcta aagatattta aaaattaaac agtgggaggt ggtaagctaa
    13081 tcaaacgata acagagaagt cgagcaggta tagagtcttc ttctaagatt agtgtatcat
    13141 cataatgaaa ttgctcgata gaaaccaaat aaaatctatc agaaaaatac tgatcattta
    13201 tatcacccgc tctactttga accctcatgg tagaataaag gctataatct gaaataacat
    13261 cactgtattc agtattcgat tggagagaat agtcttcttc tgggttggct aaattgagtg
    13321 ggattaaact aataattaaa aaaagtgaaa tcaaaatatt aaaaatagat agatttttaa
    13381 ttaatttgat tttcatgtaa agagactctt agccaccgtt ttccatttta tctgaatgtc
    13441 aactttttca aaaaaaataa ttactatcca agagaacagg gcaaaggaaa aaagatttat
    13501 cttgaatgca aataacacct aattttagaa ttatgtatca attcttaaaa gtaattgcaa
    13561 aaaattctta caaatgcccg attgattcat cgaatctgat atataaaaat tagcaaaccc
    13621 gtataaggaa ctctacaatg aataaaatga atttatctct aattacaaca ctcttgttat
    13681 tctactgtgt ttcacaagat aaaaagaaag tagaaatcga tgatacattt tacaaagact
    13741 gcatggaaac aatcaaagaa aaagaaaaat gcgatgcgtt agtaaaaaag gctgaggata
    13801 gagaaaaaga aaaagaagcc aagcaggaaa aactcacaga agagcaaatg gcaggattag
    13861 aaatcagaga tgacttcaaa aatagaatga tgggaaaatc aaaactaggt gttatctctt
    13921 ctataggaga acctgatgaa agaatatatg acggaagtgg acaagagttt tttcattaca
    13981 aaaaaccact cactcgatac agcccagaac atgatccaga caaggaaata atcgtcgtat
    14041 ttagaagaga atatgtaaca agagttctgc ataccccacc agattctact cctaaaagct
    14101 cttttccttt tattggggat aaacctaaaa aaccaaattc taaatagatt tttacattca
    14161 ttccaaccaa ttttttaagt tctgacaaga gcataaaaaa gattagacct aaaatcaaat
    14221 gattcaaaac catttttgct caaattctaa gcatattttc taataaatat aaaccaaaga
    14281 aagtatttca tcaatcaatt gctcaggatt tctccagtta gatacttttt taacaatgct
    14341 tcttttcgga aatttatttc ctgttctaca aataaagcat tttatttata aaaaatataa
    14401 tggcacgcat cttgcaaaag tcttagacag gagatataaa acgatgaaga caaaaaatat
    14461 tattttatgc ctactcatct tttcagccgc tggaatttca gctcagacca ttacaccaaa
    14521 aggctctgac ccaaaacctg cggaagctaa atggtatgac aaggttaatt tttctgggta
    14581 tgtagatgta tattataatt atactgcgaa caatagacaa ggatcaacac aagatacaac
    14641 tggaacattc cacacataca acaaacaatt tgctgtaaac gctgtaaaac tttctgttga
    14701 gaaacttcct gaaaaggaaa caccttgggg atttagatta gatatccaaa acggtcagaa
    14761 tatcatgtat caagagagac cttatcaaac atctaactct atttacaata tgcagttatt
    14821 acaacaagcg tatgtttctt tctactttcc agttgcgaaa ggcttagtaa ttgatgctgg
    14881 taaaatggca acgcatattg gtaatgaggt gttagactca aaagataatt taaactacac
    14941 gattggatac atattcttca atacaatccc tttcatccac acaggggcaa gggcaacgat
    15001 tacattgact gataaattat cagctggttt atacttatat aacagtgcac aaggaactgg
    15061 ttacacagca aacggtcagc aattcggttt tgcaggaact actccagtag gaccaaatgg
    15121 tgctactatc aatggagctc cacaggctgg cttactcaca agtccaaacc aatctatttc
    15181 tggaaaccat gtttatgtag atggaccgaa tccaatgaaa tccattggaa cacagatgaa
    15241 gtttgatgca gtgaaagata aattcaaaat tgtatggaac acattgtatg gaaacgacgt
    15301 aacacaagga agaccatctg atgcagattt ctatttatca caatcaggaa tgaatggtcg
    15361 tgttccaggt ggaccacttc ttactcaacc aaatcaagtt agcaaaaaca atagagacta
    15421 ttggtttatt aaccacatgt cctttatcat caacccaact gataaatttt caatcctctt
    15481 agattggact tttggagaaa gatcaggtta tgcaatcact aacgctgcag gatatacaaa
    15541 cgaaggtttc tatatcgact ctaacagaga cggtgtgatt gatactgtaa atggaacaga
    15601 tcttaaatac agaccaagta aacaagatgt taagagaatt tataacactt atggtatttg
    15661 ggctaaatat acaatcaacg ataagtatgc aatcggtctt cgttatgaaa acattgacga
    15721 tagtagatat ggtggatctc tcgcagttaa cgctcctctc gcgttcacaa acccaagaga
    15781 tcgttatgac ttgcaagctg catctggtga ttcaggattt acaaattcca gatatgcagg
    15841 tctaggaaga ccagcttctg gacttggcca agctagaact cttacactta caccgatcat
    15901 caacttcaca gaaaacttac aagttaaaat tgacttgaga agagactggg cacttggaac
    15961 acagttcata gatccatctg gtcgcgcagt gaaaggtcaa aatggaatca tcattggcgt
    16021 ggttgctaag ttctaaggaa aaggagaata atatgaaaac aagaaaaact cttatttatg
    16081 tggcaatggg attgatgtta tcaatacttg ctaactgtac tgaaagtagc aaagcaggaa
    16141 atgatacaga taaaaacaat gcattgttat tagcgatact caatcgtaac acagcaaatg
    16201 cacaagcacg tcaagcatgc gcaaatgcag tctatttaat gaatcagtgt attggatctg
    16261 ggtctggttt caatccagca gttatgtgtg cggatgcaaa tctacaagca ggtggcactt
    16321 atacagatag ctctgtaaca ccagccgcaa caattaccta tacaggcact gaagcgtata
    16381 caagacttgc aagctgtgtt gctacacaga ttgcagcaac taactgtaac tttagccaaa
    16441 ataaagttgc aagccctgtg caagcaaagg cagcattctt ttcaagttgt gaggtagatt
    16501 ataattctgc atcaatcaca gcagctacaa aagctaccaa agcaccttct ggaacgatta
    16561 taacaatctt ctaagaagtt tacattacac cgcttttctt atcaaaagga aagcggtttt
    16621 cattttaaca tcctttctaa aaatctctta tattcttctt tatctctagt cattccaaga
    16681 aactgatcaa tcacttttcc atctgcattc attccaacat agagtggaag tgcgatagtt
    16741 ccaaagttat cttcttcgaa tttttgattt tcttcatgaa ccatctcttc tccatctgtg
    16801 tagagacgaa ctagaatata tcgggaaagt aattccttta catccgcttc cggaaaaata
    16861 ttttgttcca tccagcgaca gtttgtgcaa gtataaccag taaagtcgac aaatatcttc
    16921 ttacctgttt tactcgcttc ctgttttgca acttctaaat tatcaagcca tatgagttct
    16981 tccgatttag taactccatt tttcaattct gtggaaggag gaggcagaaa tgcatctaac
    17041 tctcccagag atctaccatt ggtagcagtt agaaagttaa gcgccaaggc tagaaagaaa
    17101 atagcacttg cgattctaaa tgcagttttt ccatcttcct ttttctcatg ggcaaaatta
    17161 taaaaaccaa atagataaag agaagtagca ataaatattg ctccccaaat agaaaggaaa
    17221 aattctctag ataaaatctt ccatccaaaa accaaatctg cattgctgat aaatttaatt
    17281 gcagcagcta gttctaagaa acctaaaata attttaaatg ataccatcca acttcccgac
    17341 tttgggagtt ttttcattcc tgatggaaaa agtgctaata ggaaaaatgg aatcgaaaag
    17401 gcaaaactat atccaagcat cccaaggatt gggtaaaacc attctccttt cgatgcggat
    17461 accaataccg ttccaataaa aggcattgtg caggtgaagg tagtcaatgt aaaagtgaat
    17521 gacatgatcc aaatactgag caaattattt tctgagtgaa aatttgaaat tcgtgtgagg
    17581 aagccgcctg gaactacaat ttcaaacatt ccaaatagat taaatgcaaa gattataaac
    17641 acaactgcaa tgaatagatt catgtaggga ttagcggcta atcgatttaa tccgcttgct
    17701 ccaaagatca gcgcaatgac aaaacccaga atcgaaaaag agaaaataat tccaatagaa
    17761 tacaaaagtg catccgaaaa aggttttgag gaggataatt catgtcgttt tgtaaaatac
    17821 gaaactgtta tcggaatcaa tggaaatacg cagggagtca acaaggacaa taaaccaaaa
    17881 ctaaacgata aataaaggta agatgcaatt gaaatatttt ccataataca atagtttgac
    17941 tcttctaatt ccctacaatt ctaaaaatag aagagctttt gaagagttgt atttaaaaat
    18001 aatcctggag aaagacttcg ctaaaattta aatttgtttt gaaaggtaaa gattcatccc
    18061 atgctactct actttaccta ggtatatttt taatgtcata gattcttcta atagatttta
    18121 tagtcaatca cactgagttt ttcagataaa tctatttacg attttctaat aaataaagta
    18181 aattggctat aaataacaaa tctgatttat ctactaattt gtattttaaa ttagattaaa
    18241 atagaatcaa gagtagattg tgtatgataa ttcagaataa aaacaaaggt aatttaattt
    18301 acctgctttc ttttctcatt tttctaagtt tattctgcaa tacagaagat agaaaaccaa
    18361 aagaagcaat cgtttattta aaagacaata cagatgggac aatagaagac actcggtata
    18421 actacacttg gcaaaaatgc agcatgggac aaacacaaaa tgaaatctgt gcgggtagtg
    18481 ctgtattaac aaattggctc tctgccatgc aatattgtaa gcatctgaaa ttagcaaata
    18541 aaaaataggc attacctacc tcaaatgaac ttattagtct tatctcttcc ggctatcgct
    18601 cagcggatag ttcaaatagg tttccaaata aaagcggtag tatctattgg tctgccactt
    18661 cccacgaggc tgtaaattta attggcattg tggtagatct aaaagatata aaggcggaat
    18721 acaaagtgaa gtttgataca aattatgtaa gatgtatttc taagcataag gtaattcatc
    18781 atggaattca caagcatcat gaaaattgga gcgatgaaga ttgaaagcaa cgtcacgtga
    18841 cgtcgctttc cactatgaca tcgaacattt tttatatagc agtcttgccg ctttctcctg
    18901 ttcgaatgcg atagcatttt tctagaggaa ctacaaagat ttttccatcc cctacaactc
    18961 catctccatg cttcgctgag ttcatgattg cattcactac gatgtcttca aactcatcat
    19021 tacacgcaat tttaatttcc acttttggaa gaaggtttgg ttttactctt tgaccacgaa
    19081 agagttctgt atcttcttct cttccatgtc ctgttatgcg attgactgaa atacgagtaa
    19141 tttctgcggc taacagagct tctctcactt catctaattt atctggttga ataattgcta
    19201 taattaattt cataagctct cctattagtt gagattcatt aaaccatagc cactttcgct
    19261 atgctctgta tgatctaatc cagccaattc ttctgattta ctaattctaa atccaacagt
    19321 cttttgcact atgaaacaaa taatcaaagt tccaacagcg gataacaaga tagtaacccc
    19381 cattcctttt aattgtaaaa gaaattgttg aaagtaggtc cactctcctc ccattttagc
    19441 tgctgcttgt ttcatccaag aatcacgaat taaaaaacta agggctaata caccgaaacc
    19501 gcttcccact ccatgaattc caaaacaatc taaactatca tcatatccca atttattttt
    19561 catttcgagt gcgaagaaac aaataataga agatgccatc ccaagtaaaa ttgccccttt
    19621 gggagttaca actccagccg cgggagtaat tacaactagc cctgctaaga ttccagacac
    19681 aaatcctaaa gaagtaactt ttctaaattt tatagattca acaatcatcc aagttaaagc
    19741 gcctgacgca gcagaaacat ggatcacaag accacctgca agatcaatca ctccgggccc
    19801 agaggtaaat agccaaccat cagatgccca aatccaatgg gcaagtgggc aatagattgc
    19861 taaaaaccaa agtgcgataa aaagaatata acctttaatg gaaactcttt cagcaatcgc
    19921 tccactaatc agggcaggag taataattgc aaattttcct tgaaacattg caatcacata
    19981 ttcaggcact ccattcacaa tggattcatc aattccattt aaaaagaaat aatctggatt
    20041 ccaccctata aatccaccta acgcattttt cccaaaggaa agagagtaac caacagctac
    20101 ccacaatact ccaatgactg caagtggaac aaaactatgc atcatagttc ctaaaacatt
    20161 ttttgtatga accagtccgc catagaacat agcaagtcct ggaatcatta acaacacgag
    20221 tcctgtagac acaagcatcc atgcagttgt gaccgaatca acaacttcca tataacctct
    20281 catatacctc aaatgcattt aagcattgtt aggtgaccga tcgaatcggt ttttattttt
    20341 tttaccattc cgtaaaaagt tttcctacaa tctagattta ttttgagaaa ttgaattttg
    20401 aatttacaat tgcatagatc acatacattt tatcgatact ttaaacatag aaaatatttt
    20461 gctgaattca taaggatttt ttattaaaaa tattcaggtg agataaaagt tttactttaa
    20521 attattttac tgtttttttt taaacaggaa tattgttcta aatgtagtat tactaaacgt
    20581 aaaaattatt taaataattc tgaagtgaaa aagtgtattc ggtataaaca tattactagg
    20641 agctttttta agaatatatt tattgttttt gaattcaaag ttttttataa atttaaaatc
    20701 acttctattt taaaaaccaa ttcaaatatt tagaaagtat tctgatttta caaatttatt
    20761 tagaaaactt tcttgctctt tatttaattt tatttttcgc cttttaccct ttataaattc
    20821 aaatgcttcc tcaacacttt gctttttgtt cctaattaaa taaagaatga ttgcaactaa
    20881 tgctgttctt ccatgccctt cagcgcaatg aatatatata tttgaacgaa gcttactgac
    20941 ttcttttatg aataaataaa aatcaacctc atcaggaata gaagcatcta aaattgggaa
    21001 tgatatatac tttttatttt gaataatcga gtaatcttca ttcagttcag ccgttaaatc
    21061 tacaactatt tcaatatcat ttggcaaatc tttgtatatc aatttttttc caatgtaata
    21121 gttttcaaaa aagaaatcat atttattctc ttttgaaaat ttatatttaa aaaaccaaat
    21181 tgaataaatt attaaaaaat agggaagtat tgcaagatta gagattattg aaagcttccc
    21241 tgagttttta cttttataaa ataattttgg tttatttatg aagtaaatca atccaagtat
    21301 gggataactt aaagaaaacc aaaatagaac aatttttaat agttgattat ccatttctaa
    21361 agcatttgcc gaaaatgcaa tcgataccat caaaaaaatt aaagatagct tttgggatga
    21421 tttcatgaat ctatatctga ctacgccaaa caaccattaa tcacaaatca tattctttaa
    21481 tcttccgata caaagttctc tccgatatac caagaacctt tgcacacttc tctctatttc
    21541 caccaacgac tttcaaattt tctcgaatga tttctttttc gtaatcattc attggaattc
    21601 cggggatgat atttaatttg ccgcgagtga taatagaaga ggttgtgata aaatcttttg
    21661 gaacctgtga cacatctaat actttctcgc tagaaagaat aatcattccc tcgagcatat
    21721 ttcttagctg agcaacgttc ccaggccaat catactgcaa gataaattgg atcaactttt
    21781 cagaaattct tgtgatattc ttattgtatt tatctttcaa ttcatttaca aaatgattca
    21841 cgagaagagg caaatcagac ttaacctctt tcaagctaac ggtatttaaa cagattaaat
    21901 ttattttttg aaaaagatct tttcgaaaaa ttccatcttt caccatttga ggaagacttt
    21961 tattagaaga agtaaaaatt ctagaagcaa attgaaccgt ttctttttta tctgtgacta
    22021 tctcttgatt ctctaaaatt ctaaatagaa ttgcttgtcc gtctgtatct aataattcta
    22081 gtttatcaaa ataaacagga gagtttattt tgattgattc tccttttata tgtaaggttt
    22141 ttgctgtctt ctctgaatac ttatgtatta aaatattcaa atcagaatga aaacttccac
    22201 aatcaatctc aatcatttct ttttgagaaa tcaatttagc aattaaactt tttccagttc
    22261 caacatcccc ttgtatataa actggaacat tcaattcaga taagttttca atcttaacat
    22321 ttaaagattt aattggagat gaatttccaa ttaacatctt caatccaaat tcatcctcaa
    22381 ttggtttcat ctagttttgc ttagatattt tattttgctg gctaccgttt gtatcagcca
    22441 ttttttttaa ctcttcataa ccaggtaata aggagagatc tggaactact acaatttcaa
    22501 ttctcctgtt caaagcctta tttgcatttg tatcgttagg agcaatagga cgactatccc
    22561 caaaactagc agcagatatt ttggattccg gtaatccagc ctttatcatt gtatataata
    22621 cattaatagc tctatcagcc gctaaatccc agttcgtttg accgtagact ctatatctat
    22681 cactatctgt atggccttct atttgaaact ttctattttc tatctctgtc aaaattgagg
    22741 ttacttctgt gatcgctttt ttaccgttag acgatagatc ttttgaaccg gatgcaaata
    22801 aaacatctga cggtaaaaca ataaccatcc taccatcaat cattttgatt tttaattttc
    22861 ccgtatcaat aaaagtttta aacttatcaa gtagactttt aaactcagta acacgtgcct
    22921 cggattcttc ttttcgtttt ttgagatcct ctagagctcg agtcatttca tctctttgtt
    22981 ttgctaaggc atctttctct tggcttaaat catcgacgga tccttccatt tgtgcaatgg
    23041 aacttcgtaa gaatattttc tctttgagta attcattgta tttatcttgc tttgcttgca
    23101 gttctttgct ctgtgcttct tttagattag cgatttcttt atttttatcg tcttctaatt
    23161 ttatagattt ttccttttct gcttttagct cttcttgaag cttttgttct gtttcatcac
    23221 aggtttgctt taatgtatca tacttactat tcgaaacgca tccaataaat aaaaaagaaa
    23281 agagtaaaat tattttatat tgattcattc aaaagtttcc ttaattcact cattctatgt
    23341 aaaatataag aatttgtaaa ctacttaaag ttgtgtaatc aatacagggt atcccttgcg
    23401 gataacaagt gtatgctcaa attgagcaac taaactacta tcgggagttc gtaaagtcca
    23461 accatctgat tttgtaactg catgaatggc tctggtggaa ataaatggtt ctattgtgat
    23521 caccattcca tcttctaaca ctctatcatc tgatttatca taataattag caataaatcg
    23581 aggctcctca tgaagtgcac ccccaacccc atgacctcct agattacgga taacggtata
    23641 cccactattc tgagctaagt cttgaactgt tttaccaatc tgatttattt tccttcctgc
    23701 tctagcaatg ttaactgcac tgtccaatgc tgaacgggta acgcgtatta accgctgtgt
    23761 catagcatta taaggaggaa tttgaaatga tgctccagta tcagcaaagt atccatccaa
    23821 ttctgctgat acatctatat taacaagatc tccgggggca agttttcttc tagaagaagg
    23881 gattccatgc gcaaattcat cattgaccga tatacaagta tttcctggaa aatcatacgt
    23941 tagcttagga gccgaacgtg ctcctttttt ttcaagtaac ttacctccca aataatccag
    24001 ttcttctgta tgcataccag gtctcgccgc ctgcctcatt tcgcgaagca cactagacac
    24061 aatcattcct atttttttta ataattgtat atctctcatt gagtttattg acatagtgtg
    24121 gtctccgttt tataaataaa tcctatctag taatatttac tccactttaa aataaagata
    24181 tttcttgtaa agttttgttg acaatttatg aaacaccgtt aaagtcgtat gtgatttgga
    24241 ctttttgaat ttttgtcagt gttttaaagc ttgttttaga aatttactgt tttcttctct
    24301 cgcctctttt ctcacgattt tctctaaaaa aacaaaaaga tattactttt aattatagag
    24361 ttaacttcaa aaattaatca tctatattat ttgttatgcc aactcaatat tgatttaagg
    24421 attttgaata tgaacattta tgtaggaaat ttaacctatg aggctagtga agcagatgta
    24481 agagcagctt ttgaagcatt cggagaagta acttctgtaa aaattattac agataaattt
    24541 accggtcaat cccgtggaat tggctttgtc gaaatggcaa ataaaacaga tgcgttagca
    24601 gcaatcagcg atctaaacgg taaagaatta aaaggtcgta tgttaaaagt gaacgaagct
    24661 cttcctaaaa aaagttttga taaaggaagc agcaataatt cctacggaaa tagacctaat
    24721 aaaggcgatc gtccaagaag aaacaacaac tggagataat cttttacctt tagaggtttt
    24781 agaaaaaagc acgtttcaaa cgtgcttttt ttatacctaa tatcaaaatt catttcccat
    24841 ttttaaattt taaatatgtt tttgatctaa aaacatctta gttttctcgc acgcctgcat
    24901 ttgcaatata acgaataaaa acctttcgga aggtctcgtc tttctttttg tttataactt
    24961 ccaataaata aaaaactctc tatttccaga agcacctcta atccctgagt tacaaatccc
    25021 aatcacttct gctttcattt gatattttag ataagatatt atttttttga gaactcgaaa
    25081 atgaactctg tgattcttaa cgattccctt ttcagtttcc tctggctcac attcaaattg
    25141 aggtttaatt aaactaatta cttctatttt tttatctaac gcgtcttgct tcagttgata
    25201 aatgaccgga aaaatatgta aaagcgaaat aaagctcaga tcaatcacga taaacaaatc
    25261 agaataatta aaatctaaat ctcgccaaga taaattccta acattgaatc gatcctttag
    25321 aattacttta gaattgtttt gaagcctaga tgccatttga ccataaccaa catcaaaggc
    25381 ataaattttt tcagctccat ttaaaagtaa tatttcagtg aacccacctg tggatgcacc
    25441 cagatctata cagatttttt tgtttgaatt aacgttaaac gccttaaatg aagactttaa
    25501 ttttaacgcg cccctactca catattcggg aattttatct acaacttcaa ttttaacgtc
    25561 tcttggatat ttaaatcctg cttttgtaac tttctctcca tttactataa tggatccaga
    25621 aagtatcaga gaacttgcgc gagctaaatc atcacaaatc cctcgttcca cgagaaggat
    25681 atctaatcta gttttttctt tcggcaatgt aaacaggtaa atctctaaaa aaatgagatt
    25741 tgttagattc taaggattcc gaaatagcaa ttaactcgcc tttcagatca tcgcgtaacc
    25801 ttttggcttt ctccatccca tataaagatg ggtaagttaa tttgccatta gccgcatctt
    25861 ttccaggagt tttaccaagt tcttcaaagg aactttcttc gtctataata tcatctgtaa
    25921 tttgaaacaa gagtccaact ttttcaccgt agagttgaaa gtttttttct ttttctttcc
    25981 actctttagt taatcggttt ccgatgagga gtgatgataa aattaaagca cctgttttct
    26041 ttctatgtat agagtttaac gtatcctctg agaaaaaatt aggattattc tctaattgca
    26101 aatcttcaat ttgtccagag accattccct ctccacctgc accggaatgt aacaattcct
    26161 gaatatctga gaatgtatta ctatcctcga tttccatttt tgcaggaaga taaaaaccaa
    26221 atgagttgag agcatctcca gctaaaatgg cagttgattc tgaaaatttc ttgtgacagg
    26281 ttaaaacacc tcttctataa tcatcgttat ccattgcagg aagatcatca tggattaggg
    26341 aataagtatg aatacattct accgctgaac ttaaaaataa aacgttatta gatatactct
    26401 gattttccga acttaagtag gcagacaaga ccagaacagg tcgtatccgt tttcctccag
    26461 cactcaaact gtagatacag gcttctgcta gaagtggatg aaatttcttt cttatttcat
    26521 taaaaataac ttcatggaag aaattctcaa atctatcttt tcttatcttg aataaattta
    26581 agaactcttc catgtaagac tcagattaat ttactaattc aaatcctttg aagaaaaatg
    26641 tgatctcagt atttgcattt tcatcagagt cagaaccgtg aacagaattt gcacctttac
    26701 tctctgcata catagcgcgt attgttcctg gttttgcttc tttcggatct gttgcaccaa
    26761 tcacttctct ccagtgacta acagcatttt ctctctcgag tgcagctaca acgattggac
    26821 ctgctgacat atctttgcaa agatcagcat agaaaggacg agcagcatga actttgtaaa
    26881 attcttttgc atcgttctct gcaagtttaa catatttcat agccagaatt ttaaatcctt
    26941 ctttctctat gcgggcaatg atatcgccaa catgcttatt tcggacagcg tccggtttga
    27001 tcattataaa tgttctagac attttcttcc tctttttact ttaattcttt aaattttttt
    27061 aataaagctt catttatgat ttcatgaaca tgacctgcta cgctacgtcc atgtcttgct
    27121 acttctttta caatgctaga tgatacaaaa gagttatcgc ctgatgccat aaaaaaaaca
    27181 gtctcgagtt cgggagccaa ttttttattc ataagcgata tcgcatactc ataatcaaaa
    27241 tcggttactg cgcgtaaacc tcgaatgata gcactggctc ctctctcttc acaaaaatca
    27301 actgtaagtc cattaaatgt ggtcacctct aatctagatt catctatacc tagatcagtt
    27361 attacatgat gtattaagtc tatcctttcc tctatggaga ataacgattg ttttttagag
    27421 ttagttgcaa cggcaatgat aacagtatca aataagttta acgctctacg ggcaatatca
    27481 atatgcccat ttgtcatcgg atcaaaggct ccaggataaa cagcaagctt tctcattttt
    27541 tatttcgcaa gcgggatgtt tctttggttg gtaaaccata gatattaata aatccttccg
    27601 catcatattg attgtataat tcttcttttt caaaagttgc catttcatga ctatataaag
    27661 atactgatga ctttctacca actacagaac aattgccttt ataaagtttt actttcacag
    27721 ttccagtcac aaatctctgt gtttcagaaa tataggctct catggatttc attttattag
    27781 aaaaccaatg tccattgtag atgagtcttg caaattctcc agacagatca tctttctgat
    27841 gttgagtatc tctatccaat gtaatagatt ctaagtcacg atgtgcgata tggagtatcg
    27901 ttccaccagg agtctcgtaa acgccacgcg acttgattcc aaccagtctg ttttcaacaa
    27961 tgtcaacgcg accaattcca ttctcaccac caaatttatt taaagattcc attacttcta
    28021 gaggattcat gcgtttgcca tttaccgcta cacaatttcc ttcttcaaag tccaactcta
    28081 aataggttgg agaatccggt gctttttcgg gagaaacagt gagtagaaac atttctggat
    28141 ctggttcttt ataaggatct tctaatattc caccttcaaa agaaagatgc attaaatttc
    28201 gatccataga ataaggtttt gcagcagtta cagggacggg aatccctttt tgcttagcat
    28261 attcaattag atcagttcta cctttgaaac tccatgttct ccagggtgct atgattgcaa
    28321 gtgaaggagc taacgcttta aatcctaact caaaccgaac ttgatcattt cctttgcctg
    28381 ttgcaccgtg agcaaatgcg tctgctcctt ccgctagtgc aacatctacc attgcttttg
    28441 ctatgatggg tcgagcaagg gatgttccta gcaagtatct catttcataa attgctgatc
    28501 cctgaattgc aggaaaaata aaatccctcg caaattctaa acgtaaatct tttataaaga
    28561 cttttgaggc tccagtcttt aaaccttttt cttctaagcc agaaagctct tctttctgac
    28621 ctacatcagc gcaaaatgca attacttcac agccatatgt atcttttaac caagccaaaa
    28681 tcacggaagt atctagacca ccggaatacg cgagcactat ttttttggga gctatttcct
    28741 ttttcatttc aaggtcaaat taacggaaaa ggcttttctg acaatgaatt tttagggcat
    28801 accaaaagac agaaattgag tcgaataaag cctgaaacgg caaataatga ttagccgcat
    28861 attttcttag ataaaaagtt ttgaaagata tagatcaaag tataattcca aaaatttcta
    28921 ttttgtattt cttttatacc tattaacccg cactctgtaa atgaaagact ttaatcaatt
    28981 ttgtaaaaat ctaattcaaa tcaatttcgc aaattagttt atacttaaaa agcaaatcta
    29041 aaaatttcta accatttaag cgatccattc aaaattcagg attgaatttc aaactggaaa
    29101 agagaaaacc catttaaatc ctcatgtaac tcgtttgatt caaaaaaaat tttaattcga
    29161 acattgaaag tcagattatg tccatctatc acaaggcgct atgcaatact tatttaataa
    29221 attatgcttg tcttctagct aattatggtt catactaaca tcaaatagag gcgatgacct
    29281 gttatacgat agcagcaaaa agctcttcac taacacaatt aaatcggtaa atgaatgaat
    29341 cttaacactg aatcaaacaa aaatgacagt attgataggg gaagattatt aacccttcta
    29401 ataattagct caacctgcac tggggctggc ctcggttggg gtggaatcta cgcttttttt
    29461 ggattatata aagccatgtg gtggccattt ctgttttctt ttgttgtggg tggtgctcta
    29521 cttgcatatc gcttttttaa atcttacaat ctactattac atactcagct gtttatgatt
    29581 tttatcgttc caacattact acaatggacg ttaggcggat ttcatgaaag tggaatcgtt
    29641 atactatggt ctctcatgtc tccattcgga tccttgatgc tacagggaaa aaaagaatac
    29701 ctttcatggg gtggagccta ttttctacta ctcaccgtat cccttatatt tgatggatat
    29761 tttaaatctc ttggaattga atctgtatca ccaaatgcta ttgtattctt ttatgctatg
    29821 aatattagtg ttgtatcctt actcacttta cttgcaattt tttattttgt taaaagcttt
    29881 gaggatgaac gaactgctag aacttcttac aataattatc tatctgacag agttgataca
    29941 atgctaaaat ccgttgagtt actagcagac ggcgatttaa ccagtaagat acacgtttca
    30001 gaagaagatt tagtgattca aaaattattt acaggttata atcgagcatt agaagtatta
    30061 acgaaatcct ttgatgatct agataaaaat atagcgatag ttgctaattc agcagatgga
    30121 ttgattgaat ccatggatac tctctcatct gagattagcc aacaaactgt cggagtaaca
    30181 caaattgaag attttatcaa aaaaataaaa aaggagacag aagaaaactc aaaattgatt
    30241 caattagggg caaaagaatc agaagcgaat gcagatattg cattaaatgg agtctccatc
    30301 attgaaaaat caatcagcaa aattcaatca atcggtaaag aaatggaaaa ttctagattt
    30361 attattctag agttagaaaa agaaagcaat caaattgatg aaattataca ctctatcaat
    30421 ggaatagcaa agcaaactag tcttttatcg cttaacgcat ctattgaagc agccagagct
    30481 ggagagaatg gcaagggatt tgcggttgta gcaagcgaga tcggaaaatt agcagatatg
    30541 acaacaaaat ctactaaatt aatatcagac aaactaaaag aaatcaacca aaaagcaaag
    30601 catgctgttg atatagtaaa caaaagcaac gaaaatatga gagaggaact tacttattca
    30661 ggacaagtaa ataaatcaaa ccaaaacatt gtaacaaatt caaaacgagt aagggaaatc
    30721 atttcaactc tagaagaaag aagtaaaaag caatcctcta atctccaaga tatatcagtc
    30781 aatataattc aacttctaga tagcactaaa ttctttttaa atgaaattaa gacgatgagc
    30841 gatagcttct tatctatgaa ccaaaaaaca aaaacaatga aagactctgt gaaaaaattt
    30901 aagatcgctt agtttttaaa ctctccattt tcccattttc cggattgaat gacacgatgt
    30961 ttactattaa ataatcttcc ccttccatgc agttttccat ccttaaagtc accaatgtat
    31021 aggtccttat ttttaaacgt atatgctcct ttcccgtcaa actttgtgtt aagaaaatta
    31081 ccaatgtaag attcaccatt ctttttttcg aaaacaccat gtccatttag cttactattc
    31141 ttaaaaacac ctgaatactt tccagaaagg gtttccaatt tgccttgtcc atttaacttt
    31201 ccatttacaa attttccggt atatattttt gtttttccat ttttttttat gattaactta
    31261 ccgttacctt catatttatt atttttccat tcaccttcat agtagattga tttatttaca
    31321 gcaagatctt ctgaaaccga tatacgctct ccagaaggct ttattggatt ttcttttgtc
    31381 caaattccaa acccgtttct ctttccctct ttaaattttc caatatactt atatccctta
    31441 ttgctaaaaa aaataccttc cccctcaaag attcctttct taaactctcc ctcataacga
    31501 tcaccattgg aaaattcttt tatgccatgc ccatcttcac aatttccaga aacacagcta
    31561 tcgctaaaaa cattagatgt aaataacaat aataaaagga tgaaactcaa catagttcta
    31621 atctatttct atctaagtcc tgtgtaaaat caaattttta tttactaaac gacctcaacc
    31681 tttctatctt atcattgcat tgtcaaaata tggattgagt gggatcaaaa agagtgagcg
    31741 agagctagca atgtttttcc gattggttta taagatatca aatggctaag taaactcagg
    31801 acattgattt tattagttta ttagccttgt cttcaatttc caaatttttc tgttcttcct
    31861 tagaagcgct ttcattttct gaatcaattg caaggaatta atcacggttt accacaacaa
    31921 aataatcccc tgtattcaat tgaaaaaatc ttgctttata tattaaatag aaacaaaagg
    31981 gataaatact ccatggaaat atgaattcta tataggagta taaaatgttt atttttgttt
    32041 agtctttcac tatacaaaat aattttatga ggatatttat gaaaaaaact tttttaattc
    32101 ttggactttc tctaagtgct tttctaatca attgctcttc gagacaaaca attctctctc
    32161 catcaaaaga agatagaaca tacgattata ctagctgtgg aatctcgttc acaattccag
    32221 ccggatggga attacaaact acaccgtttc ttgaactcca tgttgtaaaa ggaaacattg
    32281 atattattat tgatcctgtt gcagaagaaa cagatccaga taaaatatgg acagcggtta
    32341 aagataaatt aagtgataaa gcaggtaaac ctttaaaact cagcagaaca gagaaaggta
    32401 agaaagtatc tggaattcct tctggaacaa tttactctat gactgaagac aaaaaaatca
    32461 gtgtagatgg agatgtgcta caatgcccaa aggataaatc cgttattttc tatagcataa
    32521 gccctgttgc aacatacaga acagatagac cagcagttca aagattagta ggatctatca
    32581 aaccattaaa atagtctttt aactcaactt acctaattat ttcataacat aatataggat
    32641 tgaaagtgat cattggtttt aagaatcttc ttaaaaccaa tgaattcctc ttattttcta
    32701 ttagtaatcc acttctacaa acgataggtg attagctata tgcattgcat gagccttttc
    32761 atagtctgat ttagataatt ctccataagc aaaatgtatt tttaaatcac ctgtaaaatt
    32821 tctaaaatct tcaatagctt tatagagtcg aagcattgcc tctctttcat ctccttcttt
    32881 cggaatctcg ggagcctttg gaattggagc atttaaatca tgggtcatat aaccccttgc
    32941 agaaaataga ttagatgcga tttttccagc tgtcattcta aatagtggag atttattttc
    33001 agggtatcca atgagagaat attcaatact ctgtgcacaa tgatttaaaa tttgaaacca
    33061 actccaactt ccatttggag ttattttttt tgcagtttca atttttttta gctcatttat
    33121 tgattcttct agattagaaa atctaagctt tcgatccatt ttgtctacct cattagaaca
    33181 attcatattt gcggataacg cataagtagt aacagtgaaa gctcccgctt tctgaataaa
    33241 ttttcttcta ttcattatta atctccgaca ttctgattaa aactttttta agaagttttt
    33301 caatcatgga tgcaccatga taaagtcctg tgtaaagtaa aatacttttt ctagcgacac
    33361 caagatgcgg atctttcttc tccggcaaac ttaagttagc ctcataacct tctgtaattg
    33421 taaggattgt tctttgaata ctaagtccta taatttcatt taagtttttt ttgctagaaa
    33481 cctcttcatc aattttatcc atttgagaaa gtagatagtt gatatccttt gcctgatttc
    33541 ctttttctat caaagaatag attttctcag ctatatactt cccttttttt aaagtatttt
    33601 taaaaagagt tagctcattg ataattccaa ttgtctctga caaaaactca tttttaataa
    33661 aaaaagattt ttgtccttct ttagataaat cccgaatcat agcgcggaca ttcgaaactt
    33721 tcaatggaac ttcttcgttg attgattcaa aacagtctcg aaatgatttt ttgggaacac
    33781 cttcaattct tgcacctttt gcagaaagat taatccaatt ctttcccgat gaacgatcag
    33841 agaaccattt attgaatatc tgcatctttt cattcgagtg gtattcttcc tcattatacc
    33901 ctatagattt taattttggt agagcatgta attgattata gttatgcttt tctcttctaa
    33961 aatatctaga ttctttaaaa tttaatcttt cttctaatat ggctccttta caatgcgcaa
    34021 atccatctgt aaatgcaaga tcttgtccaa caaaatatac attcgatgct cccatcaatt
    34081 ctgccagact aactgcgtta gtcgatacag aaccaccaaa aggaatatca ccaacctcgc
    34141 aactcagatg ttttgtataa atctgtagaa gaggaaatgg agaggaagta aaaaatcctt
    34201 ttttgaatat ttttgaaagt ctcagagtat gataactaga tgttggatca aatacaatca
    34261 aaccagtccc tgagtatcct tccaagtaag aagtattaat tgcttgggga tctacactgt
    34321 aaattaaatc tggttgaata ttagatctat ctaatatatg taaagatgta tctactgcaa
    34381 ttagaataaa tttatccctg tattttttta agtcttctaa attataataa agactcggtc
    34441 ctgcccctga aactacgatc ggcaatccct tcgcaatccc aaataagcga gaaacaggtg
    34501 ttaaatgaga gacttcagga atattttgaa ttagatttcg tgtccatatt ttttcaaact
    34561 tagtgagagt tgcaatgtta acatctttct tttgaaaaaa cttctcacaa atgtatttac
    34621 attcaatgta ttctgattct ttccaagata agctcggtcg attcggcaaa aaagtaactg
    34681 gtaaaacaga gattcctttg aacgtagaaa aaagagtatc ttctgtaaat ggttttaata
    34741 gaaattttag ttgcccagat tttataaaat tagaaaaatc aatagtcgac aaggcttgct
    34801 ttaaaatttc tggataacat tccatccaaa tgatttgtat attgggctgc ttaagtgcct
    34861 cttgaatcgc ataacccaaa ccagcaccga agaaaatata aactttttct tcatttgttt
    34921 tctttaaatt ttcaatcaga cgttttgctt ctatcaatgg atcatgctta ctatgaaaat
    34981 aaattccttc aacaagtagc gtgaatgctc cagactttgt cttttctaat gatcccagat
    35041 cttttgattt tacaattttc tctttgatat catccgacag aagagatata ttttttagga
    35101 gtagttcatt catactagtt cagtaactca cattagaaaa gctttagcac aagcgactga
    35161 ttttattagt ttattagcat tatctaatat tcccaaaatt ttcgttcact cctaattttt
    35221 ctaacatagc aggctttcag tcattcagtt atttttaata tcgtcctttt taaaaatgag
    35281 aaataggaat tagaaaggat ttgccattat tccaaataaa attaagttga ttaaccatga
    35341 aaaaattatt caatatattt caatattttc attatacttt attaatctat ggattagtca
    35401 tcagtctgac tgcaatttat tttattggtg cttttcacta ttatacaatc gagaataatt
    35461 taaaagtaga agaggtaatc tattccaatc tacttaaaaa tgaaattaga gaaaagattg
    35521 aaaaagcaat taaaacaaat aggctttcaa attttgagaa agaatgggag ccttatatga
    35581 atcgcctagc catcatgata caggattatt ataattttaa aatgtatttt gatagccatg
    35641 aaaaatataa attcattcaa ttaaatgaat ccattgataa ttatacaatc acgaatgctg
    35701 ataataaata tgaaataaat atttattcca tcgaaggaaa aagttgggat tcaatttata
    35761 tacactggtt aaattatttt cttaaatctt ttcgaccaac aaataaatat agatatttaa
    35821 caaattggct tttgataatt tatccagttt ctattcttat cttaattttt tatagggcaa
    35881 aaagaaatag actcacgcct taaaaaaata agaaaggata ctatggaaaa taaaacttat
    35941 aacactcttg ctgaattctg gcctttttat ctgtctgaac attcgcataa aacgaatcgc
    36001 ctcctccact ttattggatc tacttttgct cttcttttta tatttatggc aattcaaagc
    36061 tctgatttgt atttcctact tgttgcactt tttagtggat atggatttgc atggatagga
    36121 catttcgtca tagaaaaaaa tagacctgca acctttattt atccgatcaa gtcatttatt
    36181 tccgattgga gaatgtattt ttatattcta acctttcaaa tagataaaga actaaaaaaa
    36241 tattcaattg aaagcaaggg ttagtcttct actaactcct ctattttttc tatcatcaat
    36301 tttcgaattg agctttcttt aaaagttttt aagaacccga ttaaatcttg ggataaattt
    36361 aaaagattgc ctcgaatctg tttttgaatt tcatattgag ataaaagaac ttgaatacgg
    36421 ctaatctcat tttgcccgcg ttctttttgg ctaagaattt gttttatttc tataaaatct
    36481 tttttatttg ccttttcttt taacagtata agaggatacg tgtaaagacc gtttacaaaa
    36541 tctttcattt gcatcttgcc tgagttttcc gaactctgaa aataatcaat ataatcatct
    36601 tttatttgaa aaagcattcc catattaatt ccaaaactgt gaagcttctt cttcattttc
    36661 gaatcagcat catggagtat tccagctgtt actcccactg caccaaaaag agaagcagtt
    36721 ttaccatata taatttgatt gtattctttt tgggttattt tctcattctt ttcccattcc
    36781 atttgaacca attcaccaac tgataaatcg cgtataacga ctgtaaaagc actcattaat
    36841 tcaggattgt ttaaactatt taaatgatga attccacaag caagtaaaaa atcccccgca
    36901 agtatggctg ttttatttcc atataatttg cctatggttg gtttcccgcg gcgcatatcg
    36961 gctccatcaa tcacatcatc atgtaaaagg cttgctgcat gtatcaattc ggctattgtc
    37021 ccaacgtcta accactgttt gccgtcatag cctaataatt gagtaagaaa ataatgggtt
    37081 aaaggtctaa tccttttacc acctgattca ataacttttt cctttatatc ttttaaaatg
    37141 ggtaaatctt cttttataat actatctaat tgtttattaa attttttgat gaggagtggg
    37201 acttctatct tgagtggttt tgccaatttc tttatccagt ttttattgtg caaccctatc
    37261 tatacgaata cacagcagtc tggcaacttc ttttttatgt taataactag ggatcaatta
    37321 aaagctgtag gaaattctaa attcaattcc gttggatctt ttaccttgaa caatatcagg
    37381 ggtaaatgct tgcgaactaa tacttggcat ttgcatatga atcgaggcct tcacaccttt
    37441 tgaagtagta aaagaataaa cctcatagga gtgataaaga taaaccaagg taagtcctgc
    37501 aatcgcataa ttcatgcccc gaatatgctt atcgtattgc tgatttggga aaatgttgct
    37561 gcctatataa aggctattgt tgattataat ataatctgta tcattgttta aggcactttg
    37621 tgcagcagaa aactcagcac caatcgcgcc ggcaaagcct aaaaataaaa aaccaagtaa
    37681 aattccttta aagcgttttc cctcttgaaa ttgaaataag ccaggcacta catagcgaag
    37741 aaatgctagg gaggctttag atgaaccttg ccacgatact tcttctacat cgttttcctt
    37801 aaaagaaacc gtttcggatt ttgttttgat ggacattgtc tcgaaattta cagattgaat
    37861 ttcacctgtg tagttttttc catttgcaga gacggtaaga ttcaaaccag gtcttagaat
    37921 aaaaaagatt ctttcttcct tcttactctt ttttagggtg attttcttta agtcctttaa
    37981 ctgaagctct tccttctcta atagaccagc tcctttaccg agaaccattt tgtttttatc
    38041 tagaatatga ataacgtccc cacacgtctg actccatttg ttctggagtt gatagcatac
    38101 aggaaccccg ctatagccca attcaaaact agagatatct gatttcgcaa tagtctttat
    38161 ttctttgaaa acttgaaact ttattttttt atcatcctca tttaaatagc tgcctattag
    38221 aacctggcca tttttaagta tgaccatatc agctaataat gagttaaata tgaatattga
    38281 aaataaaaca aaaagtctca tttataaatc caaagattag gaaaaataga gatataatta
    38341 atatctgcta gaattgcaag taatatatta aatttcgatt gaatactgca atgaccctct
    38401 attattttaa atactcctaa aattaataaa taatatttcg aaaaaaaaac gaattttggc
    38461 aataagtgta ggaattttaa aagtattaac cgaaaataaa aagagtataa tttataagta
    38521 tgagtaaaat ttttcttttt ttaaagaaat ttcaaaattc tgcaattttt gaaatttctg
    38581 ctaatcgaaa tttgttatta ttttcaataa aaaacattga aaatagtttt tttgaaaaaa
    38641 aaatgaaaaa ttaaaataga aaaacaaaag tttttatgga acctgtatta acaaaaataa
    38701 aaagttcacg atcactccga ttacaaatcg gtattctata ctcattttta gctcttgtta
    38761 atattttatt tttctctgtt atgatttttg aaaatcagac tgagttatta gttaaaaact
    38821 tcaaatacca atctgaaaac tttgttgctt ctgtaatgac tgacttaaat tctaaacggg
    38881 ttatcaaagc agatgattct agtatcaaag ctttaaaaga aaacctattc tatgtagaaa
    38941 tctatcaatt tcgtattttt gatattacgg gtgaggtttg gtacaacgaa aaaggcatcg
    39001 tatccaatga aaaagcagat gaagacctaa tcaaaaaagc aaaagaaatc tcagacacaa
    39061 cctctgtatt tgcatctaaa tataaattag agttaaataa agaagatttc acaatcaagt
    39121 ttttaatccc attagtcgca gagcctgaag aggctggtcc aatcttttta agcacccaac
    39181 tttctatcaa aactatgcaa gagcgtttaa caaagctcta ttatcaaatt gcaatcgcct
    39241 ttatttgggg tcttatttct catattttgt ttggaattta tatttataga gtaatattta
    39301 ctcgtgttag cattctaaaa gatgcaacgg ataaaatggc attgggagat ttaagttctc
    39361 gtgcacaatg gaagttcaat cgggaagatg agctggatgt attaggtcag gcattcaatg
    39421 gaatggcagg taaaattgaa gaaacaatta gaaccattca aaatctaaac gatgaaatta
    39481 cgaaagaact aaaaatcggg aaagaggtgc aggaaatgtt cctacctgca aataaaaagc
    39541 ttaaaaactt aaaagtggca acctcatacc gcccaatgcg agaagtgagt ggagacattt
    39601 atcatttcta taaatttcca gctggtaaaa tgaataaaaa ggaataccaa ggtattttct
    39661 ttgccgatgc atctggtcat ggcgtttccg cagcgttagt aacaacagtt ctcatcatga
    39721 gtcttgaaaa tattctactc aaatctattc atcctggtta tattattcaa aaattggggg
    39781 atattattgg gaatagattt caatcctcct ttttcgcaac tgcagtattt tttctgatcg
    39841 ctgaagatgg agaaattcta tgcagcaatg caggacataa cgctccaatc tgcttcagac
    39901 cctcaaccgg tgaaattttt tattttaaaa gtgttggacc acctcttggg atggttgatg
    39961 aattcgacta taaagttata aaaatgcctg caaaatcggg agacaaagta ttcatttatt
    40021 ctgacggttt agttgagagt ccaggacaaa acgaagaaca attttccgag aagcgtgttc
    40081 ttgatattat tgaggctaac atgcatatac caaatactga tcttttaaaa ttgttagcag
    40141 aagaattaga taaatttgcg atagattata gagatgacgt ttctatgata ctcgtggaga
    40201 ttccttgaat gcaaagaaag attctgattc ttttctttct actcatacta attttatttg
    40261 ggatggcgtc tagagcaatc gttgatgtca aaattcaaga actaagattt caacttacca
    40321 aagagcaatt actcaactac gaattatcct ccaaagtctt aaaagagaag attcgtcaaa
    40381 tgatgctaac gaaagatgat tatacgaatg agattaaagt taacatctta gaatcaagcg
    40441 ttatgaatgc acaattggga gagaccacga ccaaattatc attaatcgag aaatttggtt
    40501 tgggaattgt aaacattgtt cgtgttattt cgaccaaaag ccctctcacc ctagaagaag
    40561 accaaaatga tatgatgaaa attcaatttg gtttttatat ggaaagaact agaaaatttg
    40621 ctactgcgct taaaaaatac caagagctag aaaagactct aagaaataaa gaaagcgatg
    40681 aatatggctt cataatgctt cacagcggct tctgtattgc aatgacagga gatactaaag
    40741 gagccattga aaagcttcgc cagacagaag atatgtttgt aggaagccat ttcgcggaca
    40801 acgctcgaat tttaattaat gttctcttag aaggagaacg taaaataaat gatattaact
    40861 ctggaaccaa gacagaatca gaaaaagcaa atctattata tgagacaggt caatacaaag
    40921 aaactcttgc tcaattagaa aaaattgaaa atagatcaaa tgatcaaaat tatatgagag
    40981 caagaagtct cgaagaatta ggacaaacaa gtaaagctat ctctgaatat ctcagcctag
    41041 tggaaaaaaa agaagtcgat aaggatgttg cagtaaaagc aaatcgtcgt ctacttctta
    41101 taggcagtat ctacgaaaag aatgatgatt taactgatta ttctaaatca aatgcaaaac
    41161 agttaggcga tacagacatt gtaaagaaag tcgatgaagg atcttcctta ttattaaaat
    41221 cactcattgt tgaaaaactt acaacagaag atgcaaagac tgcagatcct gaaaccatga
    41281 aagaaatggt tgagcttaaa aaagaattag agacaatcaa attaaaagat aaagaagaaa
    41341 gaaaagaatc cattgagaaa gtagaaaaag aagtagctgt tttaaaagca gaaaaacctc
    41401 cagagccacc acctgttcca gaagaaaaat atgactctac agaaagttta attatgaagt
    41461 tgagtctgag tgacggaaga agtattcaag gtaaaactat attatttgaa gaagataaaa
    41521 tgagtgtatc cattaatact ttctccacga aaatgcctgt tacattatta agcgagatct
    41581 cactcaatca aaatttaaag cttaaaactc ctcctctcgc aattcaattg aaaagcggaa
    41641 aagtgatgtt aggttataag tttagaagag aatcaggtga tacattttta ctaaatgata
    41701 aacgtaaaga tttttccatt gatctagatg atattaaaaa aatctcaatc cagtaattta
    41761 ctgataagat ttaaatgcag catctacatc tggaaaaatt tcgaagactt tgttaagcat
    41821 tgttaaatca aaaagctcta aaagatcatc gtctgtaatc acgagtcgga tatctccgga
    41881 caaggctttt aatttattct ttaagaatac aatttgcccg agaacagaag aagaaatatg
    41941 atttgcatct gttaaatcaa taacaactcg tctagactcg gatgaaaaaa tctgactaaa
    42001 atatctatcc agtctgccac tgtctgattg caaaatggaa ccatccattt tgattaattg
    42061 aattccttct actagtttgt attccataag cgttcttttg attaaattta tttataggga
    42121 agtaaattag caagaatatt ttttgaaaaa tgaattccaa gttttggacg aatagtattt
    42181 ttgggtatta atggctgtcc ttcgaatcct gtatcattct tttttaaacc tgctctcctt
    42241 tttaaactca atcaaatgga gagtttttag attaattagc tttgttattg tattctctat
    42301 tatcgtttta tgtctcagtg cctatattgt atggattaaa gaaaaaccaa atgtaaaggc
    42361 aaatctagac aaatacagat ctgaagtttc aaactattac gattcagtca aaacaaagcc
    42421 aattcgtatt tatgataaaa actctaaatt aattggagaa ttcaatcgtc gtaattttaa
    42481 gccgattcga acagataacc tacaggaaca tgccaatata gtttgggcat tactctcatc
    42541 agaagacaga gacttttaca agcatggagg catcaattac tcagcaatcg ggcgagcaat
    42601 ccttgttaat ctcaccaagt ttagactttt acaaggcggt agcacaatca cccagcagct
    42661 ttccaaactt actctaaatt taggcgaaag aaatatttat aataaaataa cagaagcatt
    42721 ctgcacttat tatatcgaga accaatatga taaagataca attctcgcta tgtatatgaa
    42781 tcagattttt ttgggagagg gtaacactgg attagaagaa gcaagtcgtt attattttaa
    42841 taaatctgct acgaaactga ctcccgcaga atctgcactc cttgtaggaa ttattcctgc
    42901 tccttctgtt tacaatccga ttcgcaatct aggcatatcg cttgaaagac aaaaacgaat
    42961 cctaaatgac atggcaaaaa atatcaactt gcatatgaat cagaaacaag tagaaaaaga
    43021 ttttcctaaa aaaatagatg aaagcatccg ccaatttaaa aatgcctata aaattgtaga
    43081 ggtcaaggta acagagaaag taaaatatac aagtgatatt ggtaaattcg gaatagatcg
    43141 tgacttcaaa ttaaatctag ctcctgattt taatgaaagc attagacgtt atgtgctaga
    43201 aaaattcacc aatgatgaat tagagaaaac ctccttaaat atttatacca ctcttgacta
    43261 tgaaaaacaa agtataattg aagcatcttt aagagaaggc attgaagatg ttcgaaaagt
    43321 attggaaaaa gaaaaacaag cttattcaaa aaaaggaaac gatgaagaag caaaaagaga
    43381 atcagaaatc attgatagca tgaatgggag cgctgtctct ttaaatccat acaatggaaa
    43441 tgttgaagcg ctcattggtg cttacaagat ttcttccatt tacagattga atcgagcaga
    43501 agaaataaaa cgtcaaccgg gatcttcgat aaagggaata atatttgcgt tagccctgga
    43561 gaaaaaaatc attactcctt cctccatagt aaaagatgaa aagattaata taggaggtta
    43621 tactcctaaa aattggtatg gctcctataa aggttatatt accgctagac aagcccttgc
    43681 acaatctgta aatacagttg cagtgaagct tctacaagaa gttggtgtta attatttttt
    43741 ggagaaaatg tcactcatac tatcagttcc ggtctctgac ttaaaggaaa gattcggaaa
    43801 taatctatca ctcgctttag gttcagggga actcagtcca atggaattag ctattgtgta
    43861 tagcacgatt gcaaatggcg gaattagaat tcgtcccaaa aaaattatca aaataacaga
    43921 tgatgatgat attgatagaa ctccaccaga actctatgca gaagaacaag cggaacaaat
    43981 cttagatcct gtggcttgtg caatggcagt caatctatta gaatcagtat tatctccaga
    44041 aggaacttta cctgctcgtt ccaaagataa acttccacta gcagggaaga cgggaactgt
    44101 tcaaactcct aaaaaagctg ctcagaaatg gggaaaccga aaaggaattc gagattcttg
    44161 gtttgcggga atttttccag gaaacgttac gacgatatgg gtaggcaatg atcaaagcgc
    44221 tccatttcca ggatcaggag caggaatcag tggacaggtg tggctaagat atgttagcta
    44281 catgaaatca agaatagatt caggcgaaaa actgattaaa ccctttgaag gagactttgt
    44341 tcaagtagat atttgcggag agcagggaat tttattacaa gatgctcctg aatgtaaata
    44401 tccaatttat gcacaatact attacagagg aactgagcca ggagcaaact cttcaccacc
    44461 agaagttaaa attgagccac aaaaagcaaa tgcaggggca gaagaaggga ttagcttcga
    44521 agatgaacca gatgtaaatt ttgaatatcc caaggaagag cctaaggatc cagaaccaac
    44581 aattgaaaag gttcgtgaac cagaagcaaa tcctacggag aataacgttc taccagaacc
    44641 aaaaaagacc caaacggagc aataatcgtt ggcttttata aaaaatattt ttcaaaatac
    44701 tggtattgca tttaaatatt ccttatccct gtatttaatt tgcttaattt tcaatacatc
    44761 ctttagagtg atgggatttg atttgagcag tgagatcaac tctatcctga atggtgactt
    44821 ttttgtatta atattactta taaacgcaaa gattctgctt atctattata tttttttttc
    44881 agtcattcaa atcagtttag actttctaat caatgaactc tcaaaaggaa aagattcttg
    44941 gattcaaaaa agaaagagaa gtcttatcta tttagttttt attctattta cattatccct
    45001 attctttcat tcaatcatac attaccctca ggtgtatgga gaattctttt atataagaca
    45061 tacttactta cagccattac tctattttct aacggattat gtaaatccga aagtatttga
    45121 gtttttcttt ttagggttta tagcaattta tctttccttc ctattcgtag gatttattta
    45181 taaaagcgat atacggtatc tattctattt atcatttttt cttttaatta ttttttttca
    45241 tgtgattagt tttgtttacg ggatcatacc tatatttctc tcatttcaaa tcatccgaca
    45301 aataaaaact aatgtaaatc gcttcctatt acttccgacg ttatttttaa taactttgat
    45361 cattggatct tattactatt ttacttttct atttcatatt cccaaagaga gttctaaaga
    45421 ttttaatctt ttaatagtat cagcagatag cttacgtgca gacagaattg gagcaaaagt
    45481 caatgggatt agcattacac caaatataga caaattcaaa gaagaagctt tcacttttga
    45541 agatcaccat gtaactatac caagaacatt tccaagttgg gcagacttac tctctggaga
    45601 atatagcatg tctcacaaga ttcgagatat gtttccagca cctgaggaaa agaaaaacct
    45661 aggaagtcca gagttcccga ctatcggtca ttatttacaa aaaaagaaat ataagactgc
    45721 agtgttttca aatttcgcag gtgatatttt tacaagagcc gattttggat ttcaggaagt
    45781 tcatactccc aattttaatg caaaaatttt agtgattcaa aaaaatttag aatttcaagt
    45841 gtttttactt cccattctaa ctggatcctt tttaagaggt ggaaattatt ttgaagaaat
    45901 aaatggcttt tcaaatttgg gggatggaga tagaatcctt cccgatattt attcttttat
    45961 tagaagagag aaatcaaatc catttttttt aaatgtattt ttttccgtta ctcattttcc
    46021 ttattctcct ccatatccag attataaaaa atttacagat cccaaatact atggtaaata
    46081 taaatatttc aaatttgtag atcctacatc agactctaaa ccagatgaaa aggatattga
    46141 acaaatcaaa gcaatctttg atgcgtctgt ttattctttt gatgaaagtt ttggaaagct
    46201 aataaaatat ttaaagaagc actctttata tgaaaaaacg ttaattgtta taactggcga
    46261 ccatggggaa tctttatatg aagacattca tggtcatgga catggagaac atttacgtgg
    46321 accttatata actcaggtgc ctctaattat taaatttcct cacacttttt ataaagaaag
    46381 aggaactcat atatttaaag gaatttccag ttctatcgat ttagttccaa cactcattga
    46441 tttttttcaa atcgatgcca acaaaaactt tccaggacaa tcgcttatac cgattttagg
    46501 aaaagaaaat tgggatgaag aaagatttgt atattctgaa acgggtattt ggttttctga
    46561 tataggagat catttttttc aatcacaaag aattatgtat ccaagtattt taaagatgca
    46621 tagaattgtt ccagaagagg aacatcagat tatgattact gatccttatt ttagagagac
    46681 tatcgcattt gcgaaacata gggctttaat tactagcaag tataaattca tttatattcc
    46741 tacaagagat ggagtaatct atgaattatt tgatagagtc aatgatccac tcaatcaaaa
    46801 taatatagca gatatgaatc cttatattgt tgaacaaatg aaaagaaatc tatacaaact
    46861 agcagaggag aaagaagggg caaatataat tggagattat attttacccc cgcctataaa
    46921 caaggattaa ttatctccga gattcttctt tatcctcttt cgtcattgct ctattgtctt
    46981 ttctttcttt ctgctcttca tttccttctt tcttcggttc cattttttta gccttttctt
    47041 ttttaggctc ttgctttggt gcttgcttct gagcaatcaa ttgtattgag aatgtaagaa
    47101 ttaaaatggt taaaataaaa taaaatgttc ttatcactat tttctcctta tagatcataa
    47161 aatactattc tttcaaatta caaaacaaat ataactttgt caagcccacg ttcaagttta
    47221 ctattaacat ttaaaagtat agagattcaa tgaagaaatc ctaaaccact aatctttgca
    47281 acctatcaat gtaataacta tttcactggt tttttatttc atgagttcat tagcatttcc
    47341 taatatttcc aaaatcatca ttcagaatta aatcacctta aattaaattg taacctttga
    47401 aaaaataata ttagatcaaa aaattaaaaa ttagaatctc gttaagatct gttctatgtc
    47461 tcctcatcag atttatgatt tgaaaaaaaa ctgaatacaa aataaaaaaa attcttgcta
    47521 aaaaaaagaa tctctttagc atttagtccc taaatttgag gagactatat gctttatttt
    47581 gcaaacttaa catctatcat tttatttgta tttggaatat cattcgtagt tgcagaacca
    47641 tggactaagt cggaaagaaa aattgttaaa tttgaaggta agtccaattc aaagataacg
    47701 cttaacgatc attattctaa ataagatatg acatcatcct ttccattgct cttgacttta
    47761 tacaaagcaa tatctgctgc tttataaaga tcttctggat tatctccatg atcaggaaat
    47821 tcagatactc ctccactgat tgtaactggt atttgtaatt tttttacgga tgccagtttc
    47881 aaactttctg aaaaacgttt acaagcaaca agtgccgatc gagtgtctat ctcaggcaaa
    47941 atcacagcaa actcctcgcc accgcaacga cataatatgt cttcctgcct aaaagactct
    48001 tttagaataa aacctattaa acgtaaaacg tcatctccca tagtatgacc atatttatca
    48061 ttaaatatct taaaatcatc aacatcgaaa acaaataaac aaatagatcg attgtatctc
    48121 tttgctcttt taagttccct tttaaaagca aggttaaaat atcttctatt gtataaagta
    48181 gtaagttcat ccactactga attttttaca acggaatcaa ataaatttaa ttcaataatt
    48241 ttgggattgc ggatgacacg gttattttcc aaaatatgat ccaaaagggc tactctaaaa
    48301 ctaacgcttc ttccaagttt tgaacacata tcagaacgat gaacatagat ttcttgccat
    48361 aatttagctg catcctcttc tgctagctcc aagttagcta tgacataaaa caaatcggca
    48421 aaaaaaccag catttgattt tttacttaga gcctctaatt tatgaagtat aagctcttca
    48481 ttggttacgt cctcatctaa gattcgcata atttcttctg tgatctttgt ttccctactc
    48541 cgcagctctt ctaaattggc agtggcgttt attaattctt ctctagcaag gtctcgcact
    48601 ttttcctgag cagagataat cttgtatgct tctaacaatt catcattgcc gaaatcagtc
    48661 actttttcat aagcatctat tattttgcga aggtagtgaa tttctttttc tttatcttct
    48721 gaattagggg cttccattga ttaaccgcag aaacatttta tttactctgg agcattagag
    48781 cagataaatt taattaagca acgagaaaaa gaatttttaa aggaagagaa ggaagattta
    48841 ttttaagtaa tttaaaatag agatagggat tgtataaaat ttttacacaa cccctagcct
    48901 agtaaggaat tattctcctg aacctgcttt aaatccacca ccgtgaactt ctaacacaac
    48961 tccagtgata taagatgctg catcggatgc aagaaatgct gctgcaccag cgatatcatc
    49021 tggttttcct gttctaccta aatagatcgc tttcttcatt ccttctctga ttgcatctgg
    49081 aattgctgcg gtcatatcag tttcaataaa gcccggagca attgcattga cacgaattcc
    49141 tctagaggaa agctctttcg cacacgcctt tgtaaaaccg attacacctg ctttagacgc
    49201 tgcataattt gtttgaccag cgtttccatc gattcctact acagatgtta aattgacaat
    49261 gcttccactt ttttgtttca tcattgtttt gataacagct tgagtagcat tataagttcc
    49321 agttaggttt actgcgatca ccgcatccca ctgttccttt ttcattctca ttaaaagagt
    49381 atcttttgta atacccgcat tgtttactaa aacatcaaca gacccaaatt ccttaacagc
    49441 tgcgtccacc atagctgcac aatcttcttc tttggttacg ttacatgtta cagcaatggc
    49501 tttataacca agacccttca tttcttctgc tgttgccttt gttgcttcct cgttcatatc
    49561 cactattact aaattagcac caagtccgcc aaacttcatt gcaattgctt taccaattcc
    49621 acgagcagaa ccagtaataa tcgctgtttt atttttaaaa tcaatcatct taatctccta
    49681 aaattttata ttccatattt aattaaattt tccgcaattc ttatattcac ttctgtctta
    49741 gcacattcaa tcacaacacg aatggcattt tttactgcta atgcagaaga ggaaccatgc
    49801 ccaattatac aagttccatt tacacctaat aaaatggctc caccgtattc tgcatagtcc
    49861 attctttttt tcatagcttt cagtgttggt tttagtaaaa gggctcctgt ttttgctatg
    49921 ctagaatcag caatattatt ttttaatata ttgaatattg atttagacag cccttcagtt
    49981 gcctttaaaa caatattgcc tacaaagcca tcacagacaa ctacatctac ttcacgccct
    50041 gacccataaa ggtcccgacc ttcaatgttt cctatgaatt taacaggcat tttctttaaa
    50101 agatcaaatg ttttaaggga tagtgtattt cctttcttgt cttcctcacc atttgatagg
    50161 attccgattt taggctgggc tacaccaaat atttcccgag catagatttc acccatcaca
    50221 gcaaattgag ccaaatactc tggcttacaa tccacattgg ctcctccatc taatagcaat
    50281 agaggtctac cttcttcctg aggcaaagga accgctatag gcggtcgatg aaccccagtt
    50341 atccgcccaa gatacaataa agaagctgcc attgatgctc ctgtattccc tggagaaaac
    50401 ataccgacac attccctgtc ggctactaat ttaacagatt gaacaataga agagtcaggc
    50461 attgacctga ctgctgtaga aggagaatca ttcatgccaa tcacttctgt agcatgaact
    50521 attcttattt tggatgtatc gtattcgtat ttgaggagct cttcgcttag aatctcctcg
    50581 ttgccaacta aaaccaccga ataaccatat tggttaacgg catctacgga gccctcaact
    50641 aaaccggcgg gtccgaaatc gccgctcatt gcgtctacgg cgacccacat aagaactatt
    50701 cttcttgttt agattttttg atctttggag caactacaag tttgccattg taaaatccac
    50761 aagtaggaca aactctgtgt gaaagtataa atgcgccgca attcgagcaa ggaacaaggt
    50821 ttggcttccc gattgcatga tgcgctctat gtgatcttac tttcgatttt gatttacgtt
    50881 tcttgggaac tgccattttt ttcctctaaa atatatatta atgtactaag atttaccatt
    50941 ttttttgaat ttagaaattt aacaagaata aatattcttt agccctatga agagatttta
    51001 ctcttcatag ttttgctttc ttggtatttt gaagctcttt gactgtatcg attgtaggag
    51061 ataaccaatc atctagaagt ggcaatgctc ctcccggctt tgctttgaat accatatctt
    51121 tattatagtt tgcaatttca gtcgttagac caatttttct ggcaatctca atcgctttcc
    51181 aataatgctt gagtgcctga gcagaatttc cttttctttc atacactgcc ccaatgttat
    51241 tataaacagc tattaaaatg ttgtatatct cttgatgcgc actttcatcg ggagtaggtc
    51301 ttgcgataag agattctttc tcttcaaaat cttcttttag tttaatataa ttccctagac
    51361 ttgcatttag ctgatctaaa ttatagaatg catttgcacg accaatcatt acattactat
    51421 ttctataagt atcctcttca cttagattgg accattcatt gagagctgct tcataatcag
    51481 atcgcatgta ttcaatccaa cctttataat agtataattc tcttttgagt ttagaatcct
    51541 tcaggtttag ttttaatgct atatcaaact tttgcatagc attgttcaga tttcttcttc
    51601 tgttgctttc ttcttctgag agttgggata aacttttatc tggatatact tttctatccg
    51661 gaaattcaga aattttacca tcctttgaaa tgagagagga ctccataaaa acaatctttc
    51721 cctcatcaaa gaaaattcta cctggatctc catttagtaa tgtctcatcc tcttctctat
    51781 ttccatataa atctttatat gcctcataac gttttttagc attttgtaga agatcaatac
    51841 tctctgaata attattgact ctcatataat gctctgccat ttccatgaca gctaaataat
    51901 gagctgggtc ataacttgcc gcgaattcaa attgctttaa ggctctaatg gattcccctt
    51961 tattaattaa gaagcgacct ctttgatagt aaccctcagc aaattcatcc ccttttacat
    52021 cctctccatc ttctcctttt tctgttttga aaaatgctag attcaagaga tgttcaacgt
    52081 tatcttccaa atccatattt gtgacttggt cttctggatt tatattgtat ttaattctaa
    52141 cttcagatgc atccaaatcg atataaaaag atgcaagctt tgcaagaaca aagagagata
    52201 agtcatcttc tatatttaga gaatttctaa cctgtctatg gtggtttaat acaaactgtg
    52261 gattttgatc ccctttccac atttcaatgt aattgctaag aaggccacca tgaccaattg
    52321 cattggaagg aaatttttcc acaatcttat tgtaatagaa aacagacttc gcaaaattct
    52381 tttgattgta ataaactttc gcgagaccag ctgttgcttc tacgttattc tcatcctctc
    52441 catctgtaaa aacttctttg aagtagttta cggcaagatt atcattcttg taatttctag
    52501 tagcagtgat tgctggttta ttaaataagt ttgcgatatt agaatgaaac tttccaagat
    52561 taatataagt ctgattgtcg tggatatttt tttccaaacg ggcaacaata taagcacctg
    52621 ctctacgaat ctttctttct acttgttttt tgggtgatcc ggagactaaa accattaact
    52681 cattgtctgg aacttctttt ctaccaccta gcggcaattt atcattatcc caagtggtct
    52741 ttgatgaaag ctgaatataa ggaacctcta atctcttagt ccaagattca gattctccat
    52801 ttttctcagc tccaaaatct ggttcgacaa ctccgaataa cttttcaaat gcttgatcgt
    52861 attccccgat tttgttatac gccatcccgt atttatttag atactctaag ttatttggtt
    52921 caattttttc acctttacta aatgatgttt tagcatctaa caaggcttgt gttctttcag
    52981 tcttatcttt tgcaagacct gctttcttaa tatattctat accctcttta taatgtcttg
    53041 cagcttgaaa agggcgtata acgtatttgt atgtgctgat taatccaatt acacccaata
    53101 tacttgctgc tgcgattaaa acggtcctct tgattaattc tctacgacgt agctctccct
    53161 cttttgtata aattggctta gtggagataa caggaattcc acgctcagaa taaagtccag
    53221 acttatcaaa taactctacc tcatagccaa gcttctctgt caaataagcc gcaatgtctt
    53281 ctggcttttg ggatgcttta attatctcaa ttaattctct ttgctcagcc ttatgtaatt
    53341 tctcgtttgt gattacatca ataactgttc gctttaactt aggtggatac tttagaattt
    53401 cttgctggat aacagctaac tcttcatcac ttaaatcttc ttcaggctct tcttcttgag
    53461 cgagactatc taggtcttca tctaaacttt cataactacc ggtttctgaa ctaggaccta
    53521 tctcatcaat tacatctaga gagctatttg tatttccacc aacgtctcca attcctgcac
    53581 ctaaatcatc aaaagaagac tcataatctt gacctgaatc tacggcatca aagtcatgac
    53641 ctataccggt tgacggttga atatctgcaa atggatcatc tgttgattca aacccagatg
    53701 aatcataatc atccatcggc attggttcca tcgtcgtact gctatcattc gtatcaaatg
    53761 tatcaactga attagtatct tcaaatccag ctaaatcatt tgaatcacca aagctatctg
    53821 aatctgctcc taaatcaccg aatggatcat ccgctgcgtt tggttctgaa tcaaaattag
    53881 caaatggatc atcagcagct tccggttgag aagaggtaaa atcattatcg ttagtatcag
    53941 tattcgtatc ttcatcaaat ccagcaaatg gatcatccga agttggttgt gtatcatttt
    54001 gaatagtagc ggaatcatcc attgcaaacg gatcatcctc tgtaacgtta gttgcagttg
    54061 gtgtatctag atcagcaaat ggatcatctg aatcaaaact gtttgtaggc actacatctg
    54121 cgaatggatc tgtatccata gcatttgggg cggaactttt ttcgaatgca tctgctgcat
    54181 cggcaaaagg atcttcaatc tgttcagaac taatatctgg agtagatgtt tcatcttctc
    54241 caaatcctgc aaatggatca tcctcatttg ttgaaatcga atcagaagta gtttgaggtg
    54301 tttcgtcaaa agcaaaaggg tcactgtctt gaatctcttg tgttgtatct actgcttcat
    54361 cattgatagc accaaatgga tcttctgatg tagctgtatc cattgatcca aatggatcct
    54421 cagttgtatc ttcaaaatta gaattttgat caaaatccgt ttcttgtatc tgctcaggct
    54481 cgggttcttt gtcttttttg gatgcaatag gttgtccagc cataaggctt gaagcatcat
    54541 ttaataattc gtctatatct acatcgcttt catcgaagta tttcatcttt ggtggttcag
    54601 aaagatctga aatagcatct tctggtaatt caattccacc atctataggt tcttcctcta
    54661 ctgcagactt ggtttcttct tctgttataa tttcaggaag tcccttaatc ttacgtaaaa
    54721 ctcgtgacat tggattcaaa gtctcatatt taataggatc tttgtccaat ggagataaaa
    54781 tctcggcgat tttgcttaat tcatctttag ttaattttgc catattgata caatataagg
    54841 attcttatca attatcggaa aggattcact tttcgctagg attatttctc aaattgataa
    54901 gaaagagaaa gagttttttc tctcatcttt tctccgatca gaacctactc gattcaggat
    54961 aataaaatat gtcacataat tagaatcgga tctttcctga aattaggttg aaattaaaat
    55021 ttattgtacg aattaaaaaa aacttagatg acagggaaat ataaaaaaat tttacttttt
    55081 ttttcagtac acgttcgaag atataaaata gatatacgtg tactctaatt aaaaataaat
    55141 atcacacaat ttaactgaga tgcaaaaaac aaacaaaaag aagaagataa ctcgccccaa
    55201 aaagagattt caaaggaaat acttaatcct atttatcatt ttatctttat ttggattcag
    55261 tgtagtgggt caagatacgg ttgactggat tggctcttat tcgagtaaag aattggaagg
    55321 cactgaagag aaagcatcta caaacaaaat ttatttctac tggcaaatcg caagcctcaa
    55381 acgtgcagtt cctcccagat atattcgaat gatcgatata gatacttatg cacagaaggg
    55441 ggagttttta aactcgggca ttctatttac ttataatggt cttaaaaaca atgaagtatc
    55501 tatttgcgga aacttctctt cttggagatg cattcctatg accaaaaata gatttggtgt
    55561 ttttcatatt atagttccaa caaattttaa aaatagaaaa gatgaaccag tcctctcata
    55621 tgaatataaa tttaaagtag accatctatt tgattatgat attgaaaatt caaatagaca
    55681 ctcagatgga gcaggatcct actactcaga atatacactt gatacagtag attttaaaaa
    55741 acaagtctct gccaaggtaa tcgaagagga aatgacagaa gacctagact ttataactgt
    55801 tgaatttcaa atttataaac caaatgcctc tgtcatctcc cttgttggaa atttcaatca
    55861 atggaatcca gaacatgact atttgcaaaa agatagaaat ggaatcttta aattaagaaa
    55921 aaaacttaga ccgggtgagt atttgtataa ttatattgta gatggagaaa aaattctaga
    55981 tacatataac tcggaaacac gccacagaat tgaaacagat gaactttctt cttatttaaa
    56041 agtcattgat aacaaaatgt catcccttta attatttact acctgcggca aacggctctt
    56101 aacctcctca aatgtatctg tatatttggg gtgatttgtt cccatcactc tatcccaaaa
    56161 attaaaatat aaaccataat tacaattaaa gaatttatgg tgcatattat gatgtgttgc
    56221 cgtattattc catttcccaa accaatgtgt tgtaaaacct tttggataaa gctcaaatcc
    56281 caaatgccca agcacattca ttaatgtcat ataaagaaga aaagcaagta ttgctaaagg
    56341 atgaattggc attataaaca caagcattgg aacaatcaat gcttctaaaa tactctctgt
    56401 gggatgaaat gaaaaggctg cccaaggaga aggatttgta gaattatgat gaaccttgtg
    56461 aacatattta aataaaagtt tatgatgcat gagtctatga gcccaataaa aatatgtgtc
    56521 atgaattaga attgcaatta ttacactaaa tataaaatat ccagttcctc tttcggaaat
    56581 gttattgtat atatatgtat atccattctt cttcataaga aaaataaaaa gtccgacagt
    56641 tgcaaaaacg gtcatagtaa taagagaata ttttacttca taaattattt ttgatttgtc
    56701 tggataatcc atctgtgttc ttttatattg ccagatatct cttttccata tccagaaaat
    56761 tccataggct atacctgcaa aaataaaata acgtaatgtc aataatgaga aattgagaat
    56821 ccaaactatt aacatctttg aaggggaatc taaaaagcta tcaaacatat acttaatcct
    56881 ctaattagaa ttaaatcact gttaaatgag aaattccatc cctattttca ttttgaatgt
    56941 aattttttat aaaatcatct acatctttct ctgtttttaa agcaaaccaa tctcccgccg
    57001 gataacaaac ttgtatagga cccaactcac atcgatctaa gcatccactc atattaacac
    57061 gaattttact gttgggagca acctctttaa tttttatttt taaatattcc cgaattttct
    57121 tagcatttga ttttccacaa gatactcttg cattttcccc ttctcgaata ttttcacaaa
    57181 caaaaatatg cttttcataa aattgactca taatttacat tgaactcgct ttcaaaaaaa
    57241 ataaagtaat tgattactta ctaacaggaa tatggaaagt ctaattctaa atcaatttaa
    57301 aaaactagaa gaccagcata aatcgaatat atttaataaa atatatgaat caaattttac
    57361 taatctatat gctggcttac tagcattatg cgctatacat attcctatta ttatattaga
    57421 ttatttaaaa ctaaaaaaag gcatgtggga aataaattta ggttataaat atattttctt
    57481 tatgcacctt ggactctatg gctttttatt tttttcactc tctttttatc tttttttaaa
    57541 gaaatataac tactcattta aaacaaagaa tgttttttgt tttgttgttt ctctactact
    57601 tcaactttgg tcagcacttt tttcctctat cgatcaaatg atttttggag atatttctat
    57661 tttttatatt tcgattttct gtttacctgt tttttttaag ttcagagcgg aattagagtt
    57721 tttcaaatta gctttggctg agatagcttt tattctacta atatttttct ttcagagtat
    57781 tcctgataaa gcagaaggaa ttattgtcaa ttcaggtgta gcaatagtat tagcattcgt
    57841 tgcatctaga gttagtttct catttaaact acgagaactt gcctcacatg caattattct
    57901 acaacaaaaa gaagaattat actccgaaaa agagaaatct aaagaagccg ctcgaaacaa
    57961 aaatgaattt attaccagct taagtcacga actgcaaaat caaatgcgag gactaattgg
    58021 aatattaaaa ttattagatg aaactaaatt aaatcatgaa cagaaagatt atacaaactt
    58081 actagattca agcgtaaaaa gtttattaag ttttatcagt gatatctttg aatttacaca
    58141 aattgaatca ggaaaaacag aattggaaaa taatatcttt tatcttcctg attttataaa
    58201 aaaaattatt tccatttcat ccagtcaagc ttcattaaat gataatataa tcaaatatta
    58261 tatagaggat aacgttccga aatatatttc tggagacaag ataagacttt ctcatataat
    58321 actaaatctt ctaagtaatg caattaaatt tacaaaatca ggaactattg agctaattgt
    58381 atcaaaagtt cgagataata aaaatctatg ccttctcgag tttcagataa aagatacagg
    58441 ctctggaatt gaagaatcaa gattggataa aattttcgat ttgattacta aagaggaatt
    58501 atcaaattta aaaaagttta gaccaacagg tctcagtctt gctataagca aaaaattgat
    58561 tactatgatg ggaggaaaca ttgcagtcga aagtgttcgt ggtgctggaa gtaattttca
    58621 ttttaccttt cctacagaaa atcatgatga ttcacaaaag aaagaagata ctattattga
    58681 aaaaggaaca ttgaaagagg aaaccataag agatagtgac ctttctagaa aaatgaatct
    58741 aaaaattctt ttggtggatg ataacgaaat caatttaaaa cttggaaaaa aagttttcga
    58801 aaaattaaaa tatgaagtaa gcactgcctc ggatggaaaa gaagctgtag aactttgtaa
    58861 ttcaaaacaa tttgatatag tctttatgga tttgtatatg ccggtcttaa atggattcga
    58921 agcgacagaa gttataaata agtcaaaaaa agatataaaa gataaacctc taatcattgc
    58981 attaaccgcg aatgcaacca aagctaataa agaagcctgt atgaaacttg ggatggttga
    59041 ttttatttca aaaccattag atgtatctag aattaaaaaa tatctaattt tcttagcaga
    59101 gaaattcaaa ctagactaaa tttataatct tcttaatttc caaacatttt caaatgcaac
    59161 ctgcccttta aatcctccag agacctctga tttgataata tgctcaattt tataattaga
    59221 tctataatat ttatttattt ccttatcaga aatagaaaaa ggaggaccag ccaataaagt
    59281 ttgatcatac tcataagtta taactaattg cggagaacaa tttgttatat tttgaagatg
    59341 ttctacatat ctaatcctaa ttgaatcagg taatgcaact aacgcagctc tatcataaat
    59401 tccatctact tttccaagta tgttttgatc taaatcaaaa aaatccccaa caaaaactgt
    59461 gagattttca attttatatt ggattaattt cttttgatga gtaattgtcg gattctcatt
    59521 cagttcagag aaaagttctt taattgcctg ctccgataac tcaacgccaa ccacttgaaa
    59581 gtcttctgcc aaaagccatg caatatcaag agtctttcca cataaaggaa caaatattct
    59641 atctcctttt tgaatcccaa ggtctttgaa atactgaact aaaaataaat tcacctcttc
    59701 ttggtgaaag gcgatattac gatcttgcca tctttttaac caaaactctt tctccaaatc
    59761 actctcctca ttatatgcat ctttaaaaga tatgctcttt ctgtttaatt tagtttcttc
    59821 tctaaactca ttttgattga atctgttcag ttacaagatc aatgttttac tatatttgta
    59881 aaaaatattc catctttctc tattaactct acattcactc catacacttc tgttagatta
    59941 ttagaattaa atatttcatt ctttcttcca gaagccacga tttgtccttc tttcagaaga
    60001 agtgcataat taaaataaat tggaatctca tctgttctat gagtaattaa gattattaat
    60061 ttcgcatatc gtctatatga ttccagtaga gaaaaaaaat cttctcttgc tgctaaatct
    60121 aaagaagaac aaggctcatc caaaaccaat atttctggtt cagaaataag agctcttagt
    60181 aataaaactt tctttttctc tccggaagat aataaactat aattttgagt agagtcttta
    60241 atccaggtat tactctcaat tagttgatca atcttttcca attgactttg agtcaattca
    60301 ctataaattc ctattgagtt tagcaatcca gatgagatca catcttttac tgataaattt
    60361 ctctgaagtc tttcttcttg atgggagctt tgtaaaatac caattttgct ttgaattaat
    60421 tttataggat attcgccata agtcttttca aaaacgctca ccttgcctga tgtaggccaa
    60481 agatagccat ataaaagatt aattaaggta gtctttcctg atccattttt tccaagaata
    60541 acccaattct gaccgctctc cattgataga tctatatctt ttagtataaa tctatcattt
    60601 cttataaaag agactttttc taatttgagc aatttatgcg tgagatgttt taaactgagt
    60661 gtctattttt tctagatact tggtaaatac tgcttcattg tctaacttaa agtttttaag
    60721 tatttcctga ttgatagtaa agaagttcgc tcttccatct aacgcatcaa gcatcatatt
    60781 tgctagatat acaatctcta acaaatctct atgctctgct ggtgccaaaa atggtctatg
    60841 gtgaaattca actatatgaa ctaagtcatc agggaaagac cattttttaa gtaaacgggc
    60901 tccaatatca gcatgactaa ttccaaaagt aaactcctca attagaacag aactatcctt
    60961 ctcttgaatc tgaagatttg tcattttttc aacgactttt ttatcgagtg atagaataat
    61021 taacttacca atatcatgaa gaagaccacc tgtcgctgct aagtctgaaa ttttatgttt
    61081 agaattatct actgcaatta cttttgcaaa gaaactacat ttagcacaat gatcccatat
    61141 cttttgcact ttgtcataac gatttcccat gatctttcga actccagaaa catataacat
    61201 attcttgatg ttcttaagac caacgacctt aactgcagat aaaacagtat tcgccttatt
    61261 tctagtgata aaaagagaag aattggaaag ctttaataga tctgcactta aaacagcatc
    61321 tttttcaatt tcggctgtta aaatatttat atcagtgtct ggtttattac aaagattaat
    61381 aattttgctc acactttgtg gaagcggtgg aagtccatca atttcattta agatttgttc
    61441 attgaatttg ctatttgtaa caataggtat gacttttcct ggaattgtaa aatgtgcgat
    61501 agtcttatca ctttgaaatt caatattaaa gttttctctt ccaatcccag aattctttaa
    61561 aagaatctgt gtaagaatca atcctaaacc cgcactctca gtggagtcag acatatccat
    61621 aaacgcatcc gataggtcat tgtatttttt tgctgcttca atgcgtttgt gaatcctctg
    61681 ctgctcttct ggaagcacag gtgcattatt ttcagcttta atataaagtg tattatcttt
    61741 aatttttgct tcgaactgta tgtaaaattc agatttgtct aggaaatctt gctgttcatt
    61801 ccatttcatg gtcacttctt ctgcgaaaac agccatccct ttttgatagt catctggatt
    61861 ggatatatct agcgattcac gtataaaata gagtcttttt gcatttgcct tgctggcatt
    61921 gtataatatt tccttaagaa cagtaaaaga aacttctaat aagaataatt gatcgaaata
    61981 tgccaatata tggatgaaca aaacatgaaa ttcttgtaat atcttgtcgt tagtatattt
    62041 aaatcgtacg gaaacaggtt gtccttttat tagcttagaa actatatcgt ttatatctaa
    62101 aagcatcgta tgtcccttgt actaagaaca gtaagttatt gcaagatttt ttattgaaac
    62161 taaatgattt caagagattt aatttttaca aattttaatg ctattttgtc ctaccctttg
    62221 cctaaactga attcagtgag gtgaaagaaa atgataaaca aagttgcgaa acaaggctat
    62281 aaatcattcg aagatactcc agaattgcag gaggacaatt ttcaaaattt agaatcaatg
    62341 gtagaatccc ttctttcatc catgaatcaa aagaagagcg ataaaaaaaa gaagcaaaca
    62401 actccaaaaa attggaaaaa atcaaaaaag aaaaaaagat cggaagaaat tgggatcatt
    62461 gaacttccat ttactttcta atatattgaa agcaagttga acacggacaa ttcaacttgc
    62521 ttcttcaaaa tcaccagaaa atggtgctca tttccaaatc aaagtatctc ttattttttt
    62581 tccgtttttc cactctcatt tagcacatat aaatcataat ttacattatt gatttcttta
    62641 atttcttttt ccaaggtata attctttaaa aaatctccta actctgggaa tgtttgaaat
    62701 aaatcttttt taactcgaaa agtcccttct cgatttaaaa caattatatc aggtttattt
    62761 ttttgcagac tttgcatcat atcttttttg tatttatcca tagccggatg ctcattataa
    62821 aaaacatatg cttcaggata tctgctagcg agtatcatat cttgattcat aatcgcactg
    62881 agagaaacgg aatgatatgc ttcgaaaatt tgaatttttg taggtctgtt ttttacactc
    62941 tgattcaaac ttttaatcgc ttctaatgtt tccgttttag gaaaatcttt tttactttga
    63001 ttccattcat aatataaatt tgttccttta taaaggcgta atgatctagc ttgttgaaaa
    63061 ataagcgtta tgcataccaa gaaaattaca aaagaagcaa taattttttt accgctctca
    63121 ttatttagag ctatttgaaa taaataattt attaagaagg ttacaatcaa aacacaagca
    63181 aaccacattg ggaagagatg ataagcaaaa ccttttgctt gtattaaata atttaataaa
    63241 ccagtaatta gtataaaaag tggtccaata tccactttgc ctctaaacag taaaaacaaa
    63301 taaaataaga ttatgcttaa atgaaataat gataaatgac cgttattctg aaattcaaag
    63361 attggttcat ttaaaggaat aaaccaatgg gagaaagaaa aagtaaaaat atttaataaa
    63421 agctgagaag gagaaataat ctctaatgat aaagataaat ctcgaaaata agacaaccat
    63481 tgatcaaacg caacaaacat gattccttta tagagaaatg gcaatagtgt cataaaaagg
    63541 ataattaaag aaccaaggaa aatactgata taaaaagtta agttttcttt gattgaaacc
    63601 tttcgctctt ttaaatcaat gatgaagaga gataacaaaa tacatccaag caatatgatt
    63661 gcagtaggct ttattaaaac ggtaattagt atgtatatcc caattaaaaa accatgtttt
    63721 attctagatt ttccctccct tattttatca taagagatta aacttaaaat ccagaaaggc
    63781 aacatcattg tctctctttg caaggctccg aagttggttg cattttcttc taacgtcaaa
    63841 caaagagtag ctccgaagaa tgcaaatagg gttggaattt ttctatttaa ataaacaaat
    63901 gtagttccag aaaataaaat cagccaagta acatcaaaaa gacgaaatcc aaaggaagaa
    63961 ttaccaaata tttttaacgc gaaccaattt atatatatgg tcattggtaa attataagtt
    64021 aatacatcag tataaggcca tgacccctta tctagagacc atgcgaaaaa cttcattaag
    64081 accatatcaa cacagtattc aattgcccag tctaaacttt gatataagaa tcgcatagat
    64141 ctaattgcga tgattgcgat ggtaatcaaa ataaatattg agaggctttt tttcattatt
    64201 gaataagata attacaattt atattttata tgctagtgtt tatgaatcaa tatttacttg
    64261 acctgattga atgcaaatga aaaatattga aacaatgtct cctcgtattt atgtatcttt
    64321 ttatttttca ttgatgatac atttatctct aatcctacta ttcttttttc cttttagtca
    64381 ctccttagaa cttttttgcg gcattgatga agtaatgatt attccagaag agaaaaatag
    64441 ttgggaaaca atgctttcaa tcgatttttc tctagaggat ggagattcca aaaatgagga
    64501 caaaggtgat gctggtagta ttgtttccaa tcagaaaccc aaagacaatg agtcttccga
    64561 tgaaaaagag gggacgaatt cagactttag cacagagtcc ctagaaaaaa aagggtggaa
    64621 agacctagtt caaagactaa aagacacaaa gggattacgc gcaaatttca aagaagattt
    64681 caataatgta attaaagatg ggactgtagc agatagctac atacacagag aaagatttta
    64741 tgaagatatt atcgtaaaag aggtgtatcc aactgttcat tctatagaga agccatttcg
    64801 ggaggatatt aaaaatgcac ctgaagatct ttcaaaacat gaagaacgaa atgatataat
    64861 agataaattt agaaatgaaa tagttgatga accagataca attattgcat ccataaataa
    64921 aaaatctgac aaagtcacaa aatctccgtt aaaaatgtca gaacaagaaa gaaataaata
    64981 ctttgacaat tctttgactc tgcctaaaga agaacaacta tctgacttta tcgaaagata
    65041 tctgggatat gatccggata atggcgattt gccgcttatg tttagagaat tgtattataa
    65101 aaacctacaa agactcgctt atacatttag ttctgattct agttacttct caatcgatta
    65161 cctccaagag aatctcaaca aagaagatta tctcaagaat gcaatgaatc ttgtttctga
    65221 gctaaaagga acaaagaccg caacagaaat actcttcaca cttgaaaata tttatgagat
    65281 tcaaggaaat gctcttcagc atttttttat tagtcaggaa aatttaaaaa acctaactcc
    65341 cgaacaaaaa aaattaattc gatttgaaac actcagacaa tttgtaaatc gatatgcgcc
    65401 agttataaaa gataagaagt ttaacaacca tcaagatgca ctcgctcttt atttcaaaaa
    65461 gaaattagaa ataatggact acattctcac aacaacacca aatcattata gaaaatcaga
    65521 tgctcttttt gaaaaaggaa gaatttattg ggatttggga atgagaacaa ataatataaa
    65581 agaaaggaag gaagctgtaa aagtgtggag ccaaattgac gtcaccccaa atacaggaga
    65641 cttcttgaat aaagactttt ttgcacaaat atcaccgttt atacaagaat ggaaagtttc
    65701 caactccgca caatctcaat tacaaattga ttctcttcta gcaggtcgtt taatacccag
    65761 cctcatggat aagaaaaaaa gagaagaaca attgctttgg aagaaaaaaa actaaggtag
    65821 acccggaaag gaactttcat attttatttt tatatcagga aaagaattta caaattgaat
    65881 tttaaaatcg ggaaaagagt ttaccgcttg ccactttcca caagaacttg gaaaagaatt
    65941 taccatttga acctttaaat ctgggaaaga ttccacaacc tgaactttta tatcagggaa
    66001 agagtttaca atttgaattt taccatatag tttaatccca ttaaatgtgc attgactatc
    66061 tacatttcca gcactgatag gaaaccatat caataaaact aatataaata ttttcttttt
    66121 catgataaac tcctatttag attctatgat ttttccataa ttagttcgat aattattatg
    66181 tgtcaagagt ctttgtaaat aaacttctat caatattgct agtatcttat ttatttaact
    66241 ttggtttatt ctcccaagcc gcttcagata attatgtcga agtattcaaa ggacagagag
    66301 ttcaacaggg agaacttttt tacaataaag cattggactt ttataaagac tcaaactata
    66361 aagcatgtat tgataaacta aacgaatttc tttttctttt tcctgggcat ccggcaactc
    66421 ttaaatcctt aaaactttta agtcttactt ataaaaaaag agatctaatt gatgaatcaa
    66481 tccgaacaga tttactcatt tataaagaaa acccaactac agaggatggt ttaacatcct
    66541 atttagatgc agggaagaaa cttcttatga taggcagagc gaaagaaggt aaaaatatac
    66601 tagagaatat caaaaatcaa ttatattcta gtaaaatagc aaaagatgcg gaaatagaat
    66661 taaagcaaaa taagatttta gaagagaatg gattccaaat taaagaagaa atgagtaaag
    66721 attgaaaata ttaaatcaaa tttcaaataa aacattgatt cttaaataag ggttataata
    66781 tttgtaaaga aaacatattg tttttttgta gttctatgaa taattttaaa ataagagtaa
    66841 cttattaatg tcagcaccta gctttcaaat tgttaactat accgctaatt catatattat
    66901 tgtcgaaggc aagaaagaag cgtataactt ttacatcgtt agagaaggaa aagttagcgt
    66961 aggacgtgaa aacccggtag taggagaaga tcctaaccaa gttgtgggtc ctggtgactt
    67021 tttcggggta gttgccgcta tgagccaaca tccgcaaatt gagtctgctc gcgcgttgac
    67081 aaacgtttct cttatatctg taagctatga tcaatttggc acactgattc aaagaaatac
    67141 acctgtcgca atgaagatca ttcgttactt ctctatgaag ttacgacaat tcgatcaaac
    67201 tatcacaaga ctatcattca gaaatactgt tagcgaagat ttaaatcagc tctataacat
    67261 gggtgaatac tatttcagcc aaggtaatat tgagcatgcg gtatacgcat atcaaagtta
    67321 tttaaaatat ttaccaactg gacagtttgt tccacaagta aaaaagaaat taatgtctct
    67381 aaatcaaccc ttagctcttc cgcctgtcga tgaaacaaaa ttcaatcgtt cttatgcaga
    67441 caataaagtg attttctgcg agcatgagcc tggtcgtgag ttatatatta ttcaaagcgg
    67501 taaagttaag atttctaaaa tcgtaaataa caacgaagta atgttagcag ttcttcaaac
    67561 aggtgatatc tttggagaaa tggctattct agataacaaa cctagatccg catctgcagt
    67621 agcatttgga tatacggatg tattagcaat taataaagca aactttgagt caatggttaa
    67681 ggcacaacca caattagcaa gtcgccttat cacaattctt tccgaaagaa tttggatcgc
    67741 ttacaaacaa ttagcaaact taatgattaa ggatcctcat gggcgtattg cagatacact
    67801 tttaacgtta gccgaaaaga acagagctaa aattgctcct aaatcattct atgactttga
    67861 tattaccgat agagacttac tcaagatggt tggattgtcg gacaaccgag acgaaaagat
    67921 attatactca ttatttgaag caaataaatt cctaaaaatt gatcaaggta gagtcaaatg
    67981 ctatgatctt gcagaactag aaaaactagt tcaatactat cgcaagaaaa caatcatgga
    68041 ctcaaagata gcaaaagaaa aaattaggta agtcaatatg ctaatgaaga atttagcagc
    68101 aattgactta ggaactaact catttcatat catcatagta aaagtaaatc ccaatggaac
    68161 attcgaatcc cttggtaaag aaaaagaatc tgtgcgattg ggaagtggcg caggtccaaa
    68221 tgaagtaatt actccagatg caatcgaaag aggaataaaa tgtctgaaac gattcaaagg
    68281 acttgctgaa attcaaaatg ctgaaatccg atctgtcgca acaagtgcgc ttagggaagc
    68341 caaaaatcgg gaagtttttg tagaaaaagc tagaaaagaa ctaaatctaa acattgaagt
    68401 cataagtgga ttcgaagagg ctcgattaat ttattttggc attctacaag gtctaccagt
    68461 ctttgataaa aagattatgc ttgtagatat cggtggagga agcactgaaa tcttagtcgg
    68521 tcagagggga aatatcgaat tcgcacaaag ccttaagata ggtgcaattc gcctaacgga
    68581 taaatttttt gccggagatc ctgacaatga gtctgacatc aatcaatgca agctttatgt
    68641 agaaggaatg attgcgcctt ataagaggga aatcgaaaag ttagcgccag aaataattat
    68701 tggttcatca ggaactatcc aagcaattgc tcagatggtt ttagcagaag aaaatgaaga
    68761 gatgccgcgt agcattaata atttctcttt cacaaatgac caacttgcaa aagttcgctc
    68821 cattttaaat gcagcaaaca cattaaaaaa gagaacaaaa gttccaggct tagatgttaa
    68881 aagagctgat atcattgtag ccgggagcat tatcttagaa caaatattca aaacatttaa
    68941 tataaaatca atgacggttt ccgaatatgc tctaagagag ggtatcattt acgatacaat
    69001 caagaagaga gaagaaagag accataaaca agattcagaa agtgcactcg ataatattcg
    69061 attaaaagcg gttaatacct tatttacctc atttccatat gaggcagatc atgtaagaaa
    69121 agtaacagct ctttctttaa aaatatttga tgatctaaaa tctttacata aatgcactga
    69181 agatgaaaga gaatatttag aagctgcttc cctactccat gaaataggtt ttgggatttc
    69241 acatagttct catcataaac atagttatta tattattcga aattctgaat taatggttgg
    69301 ttttaatttt gatgaaatag aaattatcgc gcttatcgca cgatatcata gaaaaagtcc
    69361 accaaaacca aaacatattg aattcaataa attaaacaca aacaatcaat tgattgtaaa
    69421 aaaattatca gctattctta gaattgcaga tgcgttagac agaagtcatc aagggattgc
    69481 cgaagaggtt acttgcgaat tacaaaacga agttgttgta ttcaaagtga aagcaaaaaa
    69541 agacgtagat ccccatatag atatttggtc tgctcatcaa aaaaaggatc tttttgagga
    69601 gatctacggt aagaagtgtg cttttgtagt aatttaacta ggataaaatt ttagcagctt
    69661 ttaagaaaac tacgtagtcc tccaaagaca taaaaaggtt cgctttatct gaaagttcag
    69721 atccagcaat aatctttcca agatgtttac ctgcaatgat tgaaattttt ttagaaccaa
    69781 ataaaccaac aatcttttca aacatagata cttcatccat tgtcatttcg ggaatatcac
    69841 gaatatcaat acaacattga tttaactcaa cagttcgtag aaactccata ttaaatgtct
    69901 ttttaacttg gggcaataca tatttataaa ttccagactt aaaggttatt tttgtaagac
    69961 caggtgttgg gttttctatg agaatatgat ttttgcgtgt catgatttga tcactttgat
    70021 tctcttttga aatctccatg agttctttca ctttatcaag gagtctatgt ttatcaagtg
    70081 gctgcactaa ataagcgtta atacccaact taaaaccatt ttcaatgtcc gatctttttt
    70141 gttctttata gattccgagt atcggaatgt tcttagtgtt tgaattcttt ttaaagaatt
    70201 gaattgtatc aattaattct ttgtcgattt tttttatacc aatcattaat aaatcgggag
    70261 ttgctgtttt tacataatcc attccctttt taaattcatt tgctttgaca aaattatatt
    70321 ttgcaccaga caataaactt tctgtgttat caagatattc tgattcagga tctattgcaa
    70381 ttatcacttc cacgtcctcg tcctaggttt acttcataaa gttgccacta tataattgat
    70441 ttctgagagt attgcaagca gtttttatta aatttttgtg aaaaaacttt tatagaacaa
    70501 acttaattct atatttttgc aatttttact caaaattaac tcggagagag aattattttg
    70561 aataaatacg attagaattt gttttctcat tttctcttag aatatttaat tttgcagatt
    70621 tgtcgggata gtaataagaa gatgctccat aaattagtat actcaccgtg attcctaaag
    70681 tagcaagcaa tacatcttta ctgacttgtc gaaaggtctg taaatagggt ctgtcttctc
    70741 tcgtaactaa atgaagtata atctcacgtc cagcgagtag acctaaaaaa acccaagtcg
    70801 tagacattgg aattgtgctt tgtaattgaa atgtgaacaa aacaattcca taagcaaagt
    70861 ctaccagcgt agcagctttg gaataagcaa tgtcagattt ttcagacacc acttcttgaa
    70921 tagttccacc attggatcga ataataatag taattgcaaa gcaaataatc atcaccgcaa
    70981 aaaggaattc cgacaaagac aattttctgg gaagatagat tgcaatgttt gcagtatctt
    71041 gcaaaagcca agcaatccaa agaaaagcgg tggaagccca ctgaagactt gcccactttt
    71101 tctcctttgc cagttctggc tcaaattcaa ttaaatactc ttctcgattg taataggaat
    71161 ggattcccca aaaagccatc gcaaatagaa atgcaatgat ataacaagag aatgatttat
    71221 taagcatttt ctcaatattg ctatttcctc cgaaaagacc aaggattaga aatgtagtgg
    71281 aaataggagc ttttaggcgg gtaataatga ctagaactat aggagccatc aattgaagaa
    71341 tattgaactg attcacttct gaaaaagtat ttagccgttt aaaatgaatt tctccatcgt
    71401 atcgcatcca accatataga tggactgcta ttaaaagccc ccctagcatt gccactttgg
    71461 gaaaccaatg aacttttctt ttactttcga taaaagttcc aactgtctga attgcatcat
    71521 tgcccgcaac agaaaaggca gctacagcaa atgccatcca acctaaataa taatgagata
    71581 ctccataaaa atatccgaac agaatcagta atgctgtgaa tatagttata gaataaaacc
    71641 gaatttcatc ggcagtggat ggcttgggat ttttcatttt tgttacctta gggatagtat
    71701 tcaggataat tattacaaat taattacaag gaaaatacag ggatatattt cctaaggtat
    71761 aaaaatcaat tggatttgcc gaagaaggga ctcgaacccc catggattgc tccgctggta
    71821 cctgaaacca gtgcgtctac caattccgcc acttcggct
//
LOCUS       DAOQOS010000010        71562 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_121_length_71562_cov_7.030780, whole genome shotgun sequence.
ACCESSION   DAOQOS010000010 DAOQOS010000000
VERSION     DAOQOS010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 71562)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 71562)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..71562
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_121_length_71562_cov_7.030780"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1714
                     /locus_tag="PLF66_03585"
     CDS             <1..1714
                     /locus_tag="PLF66_03585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63151.1"
                     /translation="ERAVNSDEEAFYKNFQWLLYEKRFVVIIPASAKLDSSLTFQEAL
                     FIIAIGNGLKGMMSLKPYCSGSTASTCGGASNGTWQKNTSVSLRDNTASYKDFTASKF
                     SSVAGDSVVYVEGWGYGAAGNLPFQEAFVLQSNAVWPLLIPNPSQVYGLIPPVISFNF
                     PVLERLGFTTSATVIPSSTSTYWEQRNKLLPLIAALAKTLDDQADQTVAGKNPFTLLT
                     DLSKILSRPYVFINTDNVILPNPQYTVKPTIVDIRLTDDNPIRSATATPAEYLPSSII
                     FSPVSFLVESQRKFQDGALVLAAQTDFITNLVQMFVALGDSSKKSGRTKIWAAVQKLL
                     GEIKINSDGATSSIQYNLQQFVIDKVAVLAAYPDTRSTNVTDSSWNGVNDSVAFARDY
                     FSKTSGYSIVKNIDFILGLIIDVTPTSSEVTSLFNMLDSLLTKESNGYYNLTNILTLY
                     VPPVLIQTAPYGRNLFGVFYSLAIPGGFVDYMENKMTFMPYSIANFISDTERLLNSSM
                     ITTTSNTPDSLLYSSGVLIRVFADIYQYGKKLEKQGLSFADNLNVNESTSVFQRLNII
                     FSIK"
     gene            1805..4870
                     /locus_tag="PLF66_03590"
     CDS             1805..4870
                     /locus_tag="PLF66_03590"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HMZ63152.1"
                     /translation="MKTIAKKISLSISILFFLLFLGCNPAAGGKKKNDISNLIPLALT
                     GGGSGYSEPNDGGVAPSNYNSTDTQINLPNGTTVTGNCVPAEGQAASLCLISSDNVDR
                     YKSLEIIFASGMDKKSVKTAFSLVSLDSGALPSPGGADLDDADVSDKGGRFTWLSGRR
                     LIFDPYKELKPNETYSLTISTDAKNTAGTPISSQYTTTFKTEHDFQITNLINGITLGG
                     NNDVTFNKSAPLLLISAFTNPQGAFINIAKITLFRLGTQNGYDVCVSSCSGLLTSFNL
                     NTSAVPPVEGGNTYYYQIQTKDNKTYKRFFAFNWGDVSSASGLIANAATGVLDEGIMM
                     KLLGQVVERFVKTDFKVKEGTQNKTFNDFMGLPTTSTKRTSHCINYGSFSFIRTYGDS
                     GSALGDGYCGGSGENPGAFFGAYNGILSWGSDVFFDMDVYISGASIPGLTNGVQTVEA
                     AMNATSTDNLQIALKGRKAFINLEVVAKNRSGLGCVGICAIPSGSRFHFKTTVELNQS
                     PTPTSLRTAITNNNISVNNGVLSLGIKPFTRGNVNDPNFTMAEWDSNLAVGGMTLVNS
                     DSWVSELLSFITNMIADGLVPQVRPKIVHAMLEDVVEKVAPNVLNAVLGSLQNPGINL
                     NLPNYLPAPLANFPLNVAMKLQNDAQVRVIGANKGLVSSVDLGITYAGLVPVGGYRVQ
                     GGQQGIVSFKPPGALDSTYPFSQISVNPGMLLSLHTDSVTQAAYHMWRARAIDLNMDE
                     TFIDTINQYAGTSDLFKLTKTLLKASPIISILAPGRSTLIGVKNGPFTRLPPIGDQDP
                     ITFALEPIQAPVIKLVPPVGIGIPQVRANLTDLQMAIVGKRTSSAEYPCSAGPITARD
                     TGTSCFYTISTVRVSIDAMASFGFKTFSNPTSNPSLNNINAIYVQIDPNTNFYYSIDV
                     REGNTVNPYGLDPEAIRSVVDPLVKSLVVPLVNNILKEIPLPPKLSFPALVDKNAGAS
                     GGTNCQLNVQTTAINLSTIATPSTESYVLNKVQPLGAYATNPGALVECP"
     gene            4929..6134
                     /locus_tag="PLF66_03595"
     CDS             4929..6134
                     /locus_tag="PLF66_03595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63153.1"
                     /translation="MTELRKYLLAHNYEKIIPKVRELISENKRNFKKHEIKEWCAIRW
                     RNLFINEAAKIPKAVSAASHTKLFGGKDHRSDKQKVAERFLKEEGPNEWRTEMPDVEI
                     DELRHTTLIFCPGFLNGLFPVMAFEDAFPLLEKNYKLRVIQSDSHPMRSCEGNTIDIL
                     NAIEKGIGLNAKAELIDERHAVPPKDIFIIAYSKGMPDLLHLLVKRPDLKKRIKCIFN
                     WAGAPGGSYLANTLYDSIKDINFNIKDEFDNLIKLISPIIHVPHKIRRLSEYNVKAAI
                     LDLTTEFRKEFLRDNLKKIDSLNIPIFNITASTTITEVPYFQLQGVNELNKYDANNDM
                     QVTQKHSKLHIPMATDLAMLHGHHWDVSYNSFVGKLSFGSPNLDHPFPKVAGAIAMVK
                     LAYELGLIE"
     gene            6513..9104
                     /locus_tag="PLF66_03600"
     CDS             6513..9104
                     /locus_tag="PLF66_03600"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HMZ63154.1"
                     /translation="MKKNILILFPILTSLYCLEAKKSPFDATKNVNSLLSWVAVLSGF
                     GNNSTTSSLNVVSTSPADNDTGVTANAQIAITFNTTMDKTTITTNTDNTTCSGTVQIS
                     FDNFATCIRMRSSITTTDNKTFILNANPTMGTYATHKIRVTTGATSQTKEKLSADYSH
                     TNGFTTATPCTVYNAQTCPIPFSTIPTLSFSLGNGGLVIPIESGTAKGKILVLQGGGT
                     SNTNIYDPDLGIFITGPNLPSVIANQDSGTAFSINSGTNVGKVLIVHSVSNISSIYDP
                     VSNTMQVGPNLTSSLAWSNNFLVNSGINSGKTVILSQTVTTNIYDPISNTFSVGNNLN
                     TALSNNGSNLTVINSGSNSGKAFIVSANNLNTSDIYGLTVDGQYGIGPTFSANPFYGS
                     HSFLIPSGIQSGNILIARGNSSTDTFILNSTSLTLSTGPTLPNFIDTLADRGTHSILI
                     SSGINQGNIWLLHAGNNTSPTSFYNPATNTFSTGPTMPGLIRLNSKTVYLETGIHAGK
                     YLTIYGSNVKFTSLYDPIKNALDASSILPSAAGVGVNRIPITTGTNAGKMLVINGGNN
                     TSSLFDFASGTFTAGPNLTGVMGRSPGLFTIYGGTQDGKTLVVHGGGLTTTSLYDPIA
                     NTFSAGPALSTAAHNITNECIFKVPSGAQAGKYLIIHGNVSQNTSLYDPSTNTFAAGP
                     TTGGIIGLGATCFPINSGANAGKFLVIQGFNSTPTRVYDPSTNSFAAGPNLTIATTAG
                     TFSMPITNGSNNGKALVIIGTGNTTNLYDPTTHTFSVGPNLSGSASSIAPSSYLLLSN
                     GANKDKYWIMHGTTSSIYDPNTNAISSGPAIPDGGSLAFNITSGLFNQGVLLITGSGN
                     LTSVLYP"
     gene            complement(9219..9620)
                     /locus_tag="PLF66_03605"
     CDS             complement(9219..9620)
                     /locus_tag="PLF66_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HMZ63155.1"
                     /translation="MIAELEKIQAGWKEVKDYLSIPHSKKEYNKLVSLLDLVIDEVGN
                     KQNHPLAGLLEMLGSLVEAYEEENFKPIDAKPIEVLKYLMQEHNLSQKDMKQLGSQGV
                     VSEILNGKRELNVRQIKELSKRFGVSPAVFI"
     gene            complement(9624..9914)
                     /locus_tag="PLF66_03610"
     CDS             complement(9624..9914)
                     /locus_tag="PLF66_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016761939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HMZ63156.1"
                     /translation="MHIISWKKLSDFIIDYPNSESSLKSWYKIIKESDFFNFNELKHT
                     FRTVDQVGNLTVFNVNGNHFRLIVAIHYDRQKVYIRDILTHSEYDKNKWKEK"
     gene            complement(9981..12728)
                     /gene="polA"
                     /locus_tag="PLF66_03615"
     CDS             complement(9981..12728)
                     /gene="polA"
                     /locus_tag="PLF66_03615"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012387693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HMZ63157.1"
                     /translation="MKLIIIDGHAFVFRAYFAFQSANLTNSLTGKPSGAVFGFFKMLF
                     KIINDFKPTHIALPFDPGTPLERSKLFDAYKAQRKPMPEDLKPQVGEVIEISRELGFP
                     VMQIAGQEADDIIGTLCKKYGNKKNEVLIFSGDKDLYQTLNPNIKMYRGTKGATEFTE
                     IDEKWVFENIGITPDQVTDYMGIVGDSSDNIPGVNGIGEKGAAKLIAEYKTLEGIYKN
                     LSQIKNPSLKEKLEKNKDNAFLSRELATLKIDLTIKETLDDLILPDYLASDKIIKLKE
                     RGYNRLYTDLAKQAGIKPDTTPVKSEIEPNKENTKAANGKYSRIKTESELVKLVKEFQ
                     KAKVICVDTETTSVDPMRAELLGIALSKEEEVGYYIPIAYSQSLFASGCLGLDLVINH
                     IKPLLENSKIEKVGQNIKYDMIVLKNHGIDLENVSFDTMLASYCIRPSIRRHGMDDLA
                     EDYLNYKTITYDELVGTGRKKQELFNIDPDRVTEYAAEDADITFRLYNKLKKELKISE
                     SENTFYQMEMPLMPVLLDMEMQGVRIDEKYFKDLSTKFQKEIEKLEKRIHIHADKNFN
                     IASTKELQKVLFEDLKLPAEKKTQSGYSTDHSVLESLLGMHPIIEDLLEHRKYTKLKS
                     TYIDTLPALINPRTKKIHTNYNQTIAATGRLSSTDPNLQNIPIKDEEGKMIRRGFIAD
                     GKEFQLLSLDYSQIELRIMAHFANDKNMIRAYEKNADIHNETAQALFGVSAKEVTPEM
                     RNKAKIVNFSVIYGVTAYGLSQNLKVSRQDAGIFIDKYFHQYPGVKKYMEDICVSCEE
                     KGYVETITGRRRYIPEIKSTKRQEQEAGKRVAINSPIQGTSADMIKLAMISIHQKLQK
                     KKLKSKLILQVHDELVFEVHKKEKDTVYELAKKEMESAIKLKVPIIAQGKFGDNWEEA
                     H"
     gene            complement(12730..13812)
                     /locus_tag="PLF66_03620"
     CDS             complement(12730..13812)
                     /locus_tag="PLF66_03620"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012387694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HMZ63158.1"
                     /translation="MILHLNNSRTWRGGEQQLYYLVCGLAERKIPQILIGQPNSELES
                     RISEKVKFFPIKMKGEYDLIAAYKIAKIAKENQVKLIHTHTGATHGLALLAKFFFPSP
                     KLVVSRRVDFHINKNPLSLRKYYSKRIDYFLTVSNRIKEVLIEDGVRPEKVVTVYSGI
                     DLNKFKEAGNRNKLLKEFNIPSDTVIIGNVAALVDHKDHETMLRSIALIQTEKKFIFF
                     LVGAGELETKLKNLVMELGISDKVVFTGFRNDIKDFYAFFDVFTLTSKEEGLGTSVLD
                     AMANELPIIATTGGGISEMLEDGNGSLLAPVGDYTRLAAHYKKLIESKTLRKEYGKRN
                     KESVKAFSVENTIQKTIQVYTSLIGN"
     gene            complement(13873..14925)
                     /locus_tag="PLF66_03625"
     CDS             complement(13873..14925)
                     /locus_tag="PLF66_03625"
                     /inference="COORDINATES: protein motif:HMM:NF012421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HMZ63159.1"
                     /translation="MSYIHSVAKSTPSTNVKQEDVREFAKAVFRNSNLDVDKYLPVFE
                     NSKIKTRPVLKDISWYGETKTFKEKNELFVIHARDLAFNSAKLAIEQANLLPKEIDII
                     ILVTSSGFVTPTLDAPLIDLLGLSTDVKRIPIIGLGCAGGANGIARAGELSKLYPDKN
                     ILLITVETCTLTFRPADKRKANLIALSLFSDGSGSMIISGKKKDQSIKIETNASRKWR
                     NSLDVMGWEIEMDGLQVVFDKSIPGLITKNYLDFYNQFLEQNKLAKDAIKHYLFHPGG
                     AKVLEAFSQALDVSEDQFYYSMKVLQEFGNVSSPTILFVIEEFLKEQKFQSGEYGLFS
                     AMGPGFSSDIVLFQTT"
     gene            complement(14964..15542)
                     /locus_tag="PLF66_03630"
     CDS             complement(14964..15542)
                     /locus_tag="PLF66_03630"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004470585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HMZ63160.1"
                     /translation="MSEKDLLIQVADNSFVMFLPPDMESVRYFRKELRKTLMLNSFGE
                     DDISQIILAADEALTNSITANVSNQSEETIICRWRIVDSKFTLFILDYGKGLKISETV
                     NDSYESPKNLDHYLDDIKEHQSSSAGQLPFGGVNKTHKNMGKGLKIIRSLMDTVKILY
                     HNNETITDNPLENKINGSIVELEFNAESKNKN"
     gene            complement(15627..16058)
                     /locus_tag="PLF66_03635"
     CDS             complement(15627..16058)
                     /locus_tag="PLF66_03635"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HMZ63161.1"
                     /translation="MIVVRDIVSKEELDIAFSIRKKVFVEEQKVLLSEEYDEFEDESI
                     HILAYYQDSPVGTARMRKTDKGIKLERFSVLRDYRKLGIGSALVKNLLTKCLNELESN
                     VYLYAQLSALTFYQKHGFIAKGEIFLDAGIEHIEMELNKKA"
     gene            complement(16055..17146)
                     /locus_tag="PLF66_03640"
     CDS             complement(16055..17146)
                     /locus_tag="PLF66_03640"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HMZ63162.1"
                     /translation="MLNPRELIYETTLKQLFFRLNPENAHELSKSLLMLANSLPFVFP
                     IIEKLTTYESKRLQVEIAGIQFNNPLGMAAGFDKTGELYPFLAKLGFGHVEIGTITGE
                     EQPGNPKPRIFRYEKQSALVNRMGFNNPGSEKAFETISKQTKKIPRGINVGKTKLVAI
                     ENAVQDYVKSFKKLSSLGDYGVINISSPNTPGLRSFQEKDSFISLIKGIKEGLGGEFL
                     IPTFIKLAPDLDDKSIEELLDVILDFRLAGVILTNTTIDKTTLSKYSKVEEGGISGLP
                     VQKRSTEVIRLAYKKLKGRLPIIGVGGIDSGEAALEKIQAGANLFQIYTGYIYKGPLL
                     PYSILEYLDRFMIRQGVSKISELVGTSSK"
     gene            complement(17249..19771)
                     /locus_tag="PLF66_03645"
     CDS             complement(17249..19771)
                     /locus_tag="PLF66_03645"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HMZ63163.1"
                     /translation="MFKIFLTFFILPISIYSFPISIKDNWFVKEGFDLNFKNNPSSWQ
                     SFKELPVKSTEVKNLSDKKVIVLSFFKEVNISDSDMNQLTESVSIWLPYLSNAYEIYF
                     NGSKIAEEGIFTEDNVVRHGFHRHLITKIPTPLIHSGVNEIYIIVKGVQGEEIAVYGD
                     SNPSIDLHERNIQKNSERISLMLLFLYSFIGFYHMLLFIKRTNEKYNFYFASFCSFLA
                     IYIYTRSNAVYELGIDPMIITRIEYIFLFYITAFSLAFFENFIKGKTGLATKITFGFI
                     SILAFPMLFVEIKIAYKLLLVWQIFTLISFIGCIYLMIWGIIKKHQDSKRLFFGFIIL
                     VSTGVLDIIGAMGISKNIPNYGLLQYGFFIFVIGIAFVLANRFLRVHSQVEELNLTLE
                     KKVEERTRELQKTLTQVQALKLQQDGDYFLTSLLIKPLMVNEARSNKIKIEFFIKQKK
                     TFEFKNKTYEIGGDMCIANNIKLRGKNYCVFINGDAMGKSIQGAGGALVLGVVFRSII
                     ERTKIFKPNMELFPEQWLKVCFIELQNVFTSFDGSMLVSIVMGLVEENTGALFFLNAE
                     HPWTVLYRDGKASFLEQQLYLHKVGMIGLDGSLRVQIFQMHPGDSILIGSDGRDDIML
                     GMDPDGQRNINEDGEQFLKHVMTGKGELKGITDSILKVGEFTDDYTLIKISYIEEDKP
                     LEEIDLDYYSLVDNGDLLLAQKSIPQAIEQYQKALKIKKDKDVYYKILDTLYSQKNYK
                     EFIPLCEEYLNWNPIDSDYLFKISYAYKFEGNFSLAIDYGECLKLRDPRNVNNLLNLS
                     DLNRKIGNLARANKLLSDVMYLDPGNLKAQELQRMLEEKTMN"
     gene            complement(19844..21250)
                     /locus_tag="PLF66_03650"
     CDS             complement(19844..21250)
                     /locus_tag="PLF66_03650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63164.1"
                     /translation="MNNSVSLKKNKVNLLTFFFLTFGLGIYSPLFSVEERKSSLPSFA
                     IYTGNIPVYSTISGNFTLNPNKEISKTESYYKGLKQVTKALSGSSFLSIKVDEKVFKF
                     HELEDLRSEWNMENESITILGKIPSIPVEIAMILKKKVNREGFFYISYEAKNQSNRII
                     DIGFRSLIDVSTEANDDIPLKISIDNLQEKEVYLNEFKFTPYKSTYWETYEQETDSFH
                     GIRNYLVGNDNTPPDQIAFANWDRAFPTEWKYFTNKNINIANNPAVLLWWNVKPVKAG
                     EKREVITEFEIKKKTQPRIYFDLLDSESGSGRLHLKYINSTNSAKELSYFLDAGSNAV
                     LALNQNNPLKFIIDKNAIFDKILPISIHGKGNVNLSVKEKFGNTEIAHNLQVRLAQHN
                     REQSPKFWKSSKYPIIYNSDKDGLKLKGVVRAKDTGKKLGETDLISVRSIENSYVYLG
                     EVDLKEFSGEVLVEIFKQ"
     gene            complement(21243..22259)
                     /locus_tag="PLF66_03655"
     CDS             complement(21243..22259)
                     /locus_tag="PLF66_03655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63165.1"
                     /translation="MLFHKFKIIFLFLICLNSIFSDELIERKEAYVLSESSWFEVKEQ
                     ERQEFLKTKRERKELRKQTENQIAESEKTSKRYVSFNGDIYVNKHYKPQVVNEFKGKV
                     FIKIDDEPEQLLTPNINIYKQGIHTIVYNLYDESDVLRSTKKESIYLDTTIPELYAQL
                     QGVYYERNQFYYYKPGFKLLLQTKDKESGIEEIFIDLNRKGYLPLSKLDQPIDSPGHK
                     EIRIIAMDKVGNLSQEIHLNFTVDSDPPIVSVELDTIPENHSSKGLLCDRETLIWLNA
                     FDLGSGVHKIEFRKKGTLNWRVYRDYPLVSVKKKKISLEFRSIDNLGNQSAIKYFKCR
                     LKNE"
     gene            complement(22340..22957)
                     /gene="hisG"
                     /locus_tag="PLF66_03660"
     CDS             complement(22340..22957)
                     /gene="hisG"
                     /locus_tag="PLF66_03660"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010570514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HMZ63166.1"
                     /translation="MLTIALPKGRLADESVELLIKKKWLSEKPDESSRELTFTDSLAR
                     LKILLVKAQDVATYVEECAADAGILGWDTLIEGRYDLLSPVDLKIGKCRLSLAASLDF
                     DLKNYSRKIRVATKYPHLTKEFFFSKGLNCEIIKLYGSIELAPLCGLSDCIVDLVSTG
                     ATLKANGLEEKEIILESTARLVFNRNSIYKNRQEILEFVKDLSTK"
     gene            complement(22961..23716)
                     /locus_tag="PLF66_03665"
     CDS             complement(22961..23716)
                     /locus_tag="PLF66_03665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63167.1"
                     /translation="MAKFDRKKYREEQYKKSLVSYDDPLENFEGTKTELLFFKFSRFF
                     ARNRFQFFVGIGGFILLIVGFVAVGEWIQYNTNQATIQIEKLEKAHAKNFSIDSAKKI
                     KDFEDLLKSYSSHDAKLRIYKRISDLYVESFDFGKAADYLEKAADLVNDSKEIKAYYY
                     YVAGSHREKAKDNKLALSDFSKAATLIANNRETISLSAWTHYQSGRLKLMNGEKDAAI
                     KDLTKVLDIESESADISEVKKLATYLLIKANKG"
     gene            complement(23722..25392)
                     /locus_tag="PLF66_03670"
     CDS             complement(23722..25392)
                     /locus_tag="PLF66_03670"
                     /inference="COORDINATES: protein motif:HMM:NF004952.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HMZ63168.1"
                     /translation="MNSQTNNNRTNTSSANRGNTEQRSFKKGQIIEGRIVDVYDQEVY
                     VDLGLKSDCRVARSEFLETPEIGSMVSVVIKSKESDSEIYIASKLEADARKGWETIKE
                     AFSKNLQVQGRIESEVKNIGYNVYVEGVQLFLPASQLGMKASLDELKAKPLDFKIIKL
                     NEKGRTGVISRKKLIEEINKEKWDELVKIVKVGDKVTGTVTKVASFGVFCNVHGIEGL
                     LRQNDISYKKYAPFKQYFQVGQSVELLVLEIDPANNRLSLGIKQLYEDPWVWAGRELE
                     KDMVVRGIVTSLTNFGAFVELKEGLEGLIHCSELTWAKKPPHPKEVLKKGQEVDSMIL
                     DIDLSKKRLSLGLKQLLPNPWDNLSSEVRVGNVLEGKVTGITKYGAFVEVENGIEGLI
                     HVGDITWDEKVKDPTTLLKKGQVVKYQILDINLDTSRISCGLKQLQENPYEVLRKKYP
                     TGVIVEGKIKSIVSFGVFLEIEPGYEGLIHVSQIPDTKTQKLEEIYKVGDVIKAVILK
                     IDPDNKKISLSVKDFDKAMEKEEISKYIKSDNTPSSESFGSFINSNKA"
     gene            complement(25443..26138)
                     /gene="cmk"
                     /locus_tag="PLF66_03675"
     CDS             complement(25443..26138)
                     /gene="cmk"
                     /locus_tag="PLF66_03675"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001005055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HMZ63169.1"
                     /translation="MNENIIAIDGPAGSGKSTVAREIAAQIGYHYLDTGSYYRAFTLF
                     FYRLYKKNKINSSFADWVQAQNLEKEFSNINIETEFLSSGGNKTLLNSEDISEEIRLP
                     EITEEIKHLAPLRNVRDFVNESLHKLAKKYKLIMDGRDIGTEVFPDAKYKFFVTASIE
                     TRADRRYKELIEKGIQVDLEQLKEDISKRDKSDMERKIAPLKIANDAILIDTTQLSKN
                     VVINMILSKILQS"
     gene            complement(26145..26432)
                     /locus_tag="PLF66_03680"
     CDS             complement(26145..26432)
                     /locus_tag="PLF66_03680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63170.1"
                     /translation="MFKKLVIFFLLLIHLNCITLAGIKIGNALDGKSKNAESIIQAAG
                     LIGLFGDVYLGTAIIQPHSIGVGYSVLAALFYIVTDGGISDRIGKDENLQE"
     gene            26623..27327
                     /locus_tag="PLF66_03685"
     CDS             26623..27327
                     /locus_tag="PLF66_03685"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HMZ63171.1"
                     /translation="MENLFILLENPKYAGNIGMICRLIANFDLPPLRLIGEEKELHFE
                     MEWMAYNSKEEIAKIQYFQDKKEARADLDILIGTAMIQGKDRPGFIPIQSLYEKYKSF
                     GEKRIGIVFGREDRGLSNTMIDLCDLLIDFELSSRQPSMNLSQSVSFVLGCLHSPKPK
                     ENIAHTKDIIDKTNLYKYVQHVFELIEMNDFHGRENLAIKRFSKIIDSSSISSNDLNF
                     LFKIFKQIEYKLKKEN"
     gene            27530..28213
                     /locus_tag="PLF66_03690"
     CDS             27530..28213
                     /locus_tag="PLF66_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016546278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ElyC/SanA/YdcF family protein"
                     /protein_id="HMZ63172.1"
                     /translation="MNKLKKISQVIVYTIALFLFFSICIDLSFEINYIRINSANDYKK
                     INEATVAIVLGAAVYGNKPSPVLNDRLKSALILYKKRKVKKILLSGDNGTRSYNELKP
                     MLNYMLKNKVKKEDIFVDNSGFRTLDTLLRAKYIFQIKDAVIVTQRFHQPRAAFIAEK
                     IGIQVSCFESDSGEYKDETKNRLREFFARNLAWIDMNLMSDINYSGKSYPIIGDGTET
                     WKLKDIPLE"
     gene            28299..30329
                     /locus_tag="PLF66_03695"
     CDS             28299..30329
                     /locus_tag="PLF66_03695"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HMZ63173.1"
                     /translation="MRFLVLFFLFISQSFLFAEEAVRLTNKKGVYNVTSYLEMLEDKT
                     RTLTLSTIINSEKELSFKQIDDDTVNLGYTQSAYWFRLKLFNKNRSKPTLLEIPWPHI
                     DNLDIYMLSNKNNGDEEADDVFVSYNSGRLIPYNKRNYEHRNFVFKIPNFPNGDELVI
                     YMRVVSDETIIFPVYLYDEMKFFKKDQSEEFVFGIYYGVVIIMFFYNLFIYLSLRDKN
                     YFIYLFYVIALGLYQLSMNGIIYAYWPNYPMWNKLFLPLSSAILQFILIILFKNLLNI
                     EERYSWERKSLFVLTILSFISIILVITVDYATIIVPMNILGFIYMITSILIVVRAVIN
                     GNRTAIFFLITWITFLAGGIILTLRNFNILPQNFLTTYSLQIGSTIEMLLLSLALTDR
                     INTMKMELAILNTKLEEKVEERTKELSRVLGILQAKELTIDSELDLASDLQKCIFPSN
                     DLPYPHIKFVGYYEYLMKVGGDFYDVVPLPNNAVGILIADVSGHGIPAALLSTMYKIS
                     FMNATRRTSSPVKIFQEVNKNISSIMTTHDYLTALIIVIEPNGRICFSSAAHRPAFLF
                     RKKTKKLEVVSTKGMFIGMLSYASDTFEEKESKMDVGDRILLFTDGVIDAFNEKEERW
                     SSDELQKAFIKSSGLSLEAALESIKADWIKFRGNTKINDDSTLLLIEYTNKK"
     gene            complement(30336..31121)
                     /locus_tag="PLF66_03700"
     CDS             complement(30336..31121)
                     /locus_tag="PLF66_03700"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HMZ63174.1"
                     /translation="MKFIMVIIPAFIVLLASIIYTQQEFLLFRPYPLAANYTYSFPFE
                     FEELNWEVEPGIKINALHFKTKDAKGVVLYFHGNGGNLAGWGGIADDFLNRKQNLIII
                     DYRGYGKSTGTIKSEDDLIKDALYAYEYAKSIYPENQITLYGRSLGSGLAVYLAKDKN
                     PKRLILETPYNNLSDIASYHYPYIPTFLLKYKLDSESMIGSVKCPIYIFHGTEDAVIP
                     LAYARKLESKIKRDYKFFQITNAGHNDVSETKEYFMELTKIFW"
     gene            complement(31133..31858)
                     /locus_tag="PLF66_03705"
     CDS             complement(31133..31858)
                     /locus_tag="PLF66_03705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HMZ63175.1"
                     /translation="MENLNEKDFYFIYEDEKFNEEKEKVNIVEQVNINKGLVIIVYSF
                     SAEIEEIIKFGLTAILDRYGKSSLVNTVYNVLFESILNGLKANAKKLFFLENSLNIDD
                     PNDYAKGILLFKKDINKSNLRQSGERNRERNIFVRVKFDYSDDGLEIEVLNNTQLLPA
                     EESRIREKLALGEKYDDLMSYYLDNSDSTEGEGLGLVLSLILLKAEKLDPSLFRIGTK
                     NGLTFARIEIPFTSEFISKRTNI"
     gene            complement(31879..32217)
                     /locus_tag="PLF66_03710"
     CDS             complement(31879..32217)
                     /locus_tag="PLF66_03710"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HMZ63176.1"
                     /translation="MNVTIRENQSIPVIDIQGEITMYDIEPISEAINKLIQQKKYKVV
                     FNMAEVPFIDSSGVGIILRSLVSLYKFGGIIHLCSCVTAVDNIIKVSVKNTTTKIFAT
                     EEEAVKACLN"
     gene            32455..33294
                     /locus_tag="PLF66_03715"
     CDS             32455..33294
                     /locus_tag="PLF66_03715"
                     /inference="COORDINATES: protein motif:HMM:NF004824.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HMZ63177.1"
                     /translation="MERNKVWFITGCSTGFGRELSKLVLELGYKAIVTARNPKDIQDI
                     TENYPDTSIPIQLDVTNKLEIENSILKAKARFGRIDILVNNAGIGYFGAIEESEEEEV
                     RRMFEINFFGLANVTNAVLPIMRAQRNGMIINIASIGGLVSFPAVGFYNATKFAVDGY
                     SEVLAKETSHLGIKVMIVAPSGFRTDWAGRSANNTKKKILDYEESAGANMQNIRASSG
                     NQAGDPVRAVKAIVKAVESENPPMRLLLGKAALRNARNKLDSLKKEFDEWEETTVSAD
                     YPQ"
     gene            33526..35121
                     /locus_tag="PLF66_03720"
     CDS             33526..35121
                     /locus_tag="PLF66_03720"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="This anti-anti-sigma factor, or anti-sigma factor
                     antagonist, belongs to a family that includes
                     characterized members SpoIIAA, RsbV, RsfA, and RsfB;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0045152 - antisigma factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor antagonist"
                     /protein_id="HMZ63178.1"
                     /translation="MELEAKLEKPAIVTGTRQINNLLIKMKTPPAVHLPQRQPLVIGL
                     AIDKSWSMKGDKMEATIEAACSLVNWLTRHDYVTIIAYSADVQVVQPLIQLKEKSAVL
                     DKIRSIQVATSTNLSGGWLQTLRAVESAGVPNAYKRVILLTDGQATLGIKDPLQFNQI
                     AADHIGRGVSTTTIGFGEDFNETSLRDIAVNGGGNFYYVSSPEQTSEIFFREFGDIGA
                     LYAQAVEVKLKLAPNVKMLEIYNDFPFQSNEDGTISIQSGDVRADDARSIVMSLEIDA
                     EKNIDAPDLLNIDVSYYNLFEKMRLDKISRSLPLVKTAKEPDSDSEVVVERLVTSSAK
                     TVIKAAKLIKENDVGMARTLLQAAMDRVEDNIKLSPDILNPILQRLKNIEIKLRENAA
                     SASKHFMAEGSDLYSRMDIIDTGGVDVHDRIFEYKVIGDIDLYKCPDIKATVQSQMRD
                     GYRFVIFDLSDCKFIDSSAIGAFIQIVGWLRKRGGEFIVTNIVDSVRKVFTITRLENH
                     IRVATSMDEARDIVESIISATSR"
     gene            complement(35544..35744)
                     /locus_tag="PLF66_03725"
     CDS             complement(35544..35744)
                     /locus_tag="PLF66_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002744936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63179.1"
                     /translation="MYYSDMNENLKSSFAKNNLSNANLTSESQLPNFGLKASELLNTR
                     QMQKLAKIRAKRIKNARNFIKD"
     gene            35937..36170
                     /locus_tag="PLF66_03730"
     CDS             35937..36170
                     /locus_tag="PLF66_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020769226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63180.1"
                     /translation="MCIKLVNHTGKREGKFVSYEFGTDLFGYIYLDKFKGNDDKGKLV
                     DRWILNDLGSLVRTLDLEIYRREVENYENVNAI"
     gene            36160..38028
                     /locus_tag="PLF66_03735"
     CDS             36160..38028
                     /locus_tag="PLF66_03735"
                     /inference="COORDINATES: protein motif:HMM:NF012700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63181.1"
                     /translation="MQSKIAFHDTFTSSQFMKNSDEHKTLLSLLKKRINKLSQKIQTE
                     QKKLSKLSLLRLGIFSIIGISIFIYYTSRTDNHYYLLIIGLEIITFVFLIFYYRRIEV
                     YKNRLIAYDRLLKRETNRSQFKLETLYDYDVTINSLELKNHFHRDLGISGKNGLFTYL
                     NTTKTINGEKIFLENLLRIKRESTDEIVKRQSAIKTLSQNKNLCFKILRIFEEAHLEE
                     VNSKINLAPLYSYQSKFFEKQKILSRIYQPYIILGWLVNIYLFLAGSPSISASLFIIN
                     TILFIINRKEISHLFKSFKNIEDEIPSIERLTLYLYGSKIQFNDKNTFSNLDKNRIKN
                     LYKKLSSIIGRVSVQNAPLMHYTLNMLFLWDLWQLTSFSKWQKQNREIIQSITDDIYQ
                     IDSILPFAFLKFHNPGYTFPTLDEKLKTISAQNMGHPLIPERNRINNPLQKIKIGESL
                     IITGSNMSGKTTYLRTVGINAILALCGSPVAADKMDLPPLHILSSIKNEDSLEEGISF
                     FYSEVKRIAHILNQTKEPGIYLILLDELLKGTNTRERLIASRAILKKLASSNSIIFIT
                     THDVDLAIKTTKQKVAHFTETIEENKMSFDYKIKDGVIKSTNALKILEIENLDLDF"
     gene            38260..38913
                     /locus_tag="PLF66_03740"
     CDS             38260..38913
                     /locus_tag="PLF66_03740"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HMZ63182.1"
                     /translation="MLETEGLEHVPKAGRVMIVPNHSGILGWDATVLQNEILREKKRI
                     PRTMAHAFWESQPFLKKVSRKMGLITTDFKLAVKTLKKNKLLVIFPEAEHGNFKPSHK
                     MYQLTDFNPGFVALSLMTETPIVPVAILGAEENYINIGTIDWFEEQIGAKIPIPLNLL
                     PLPSRWKIIFLKPISLKKYSRKDVKNEKFLIEVAQNVRFRIQAAIHKELVNRGIFKF"
     gene            38926..39369
                     /locus_tag="PLF66_03745"
     CDS             38926..39369
                     /locus_tag="PLF66_03745"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HMZ63183.1"
                     /translation="MVDISTLSRTELLKKLKVEDLMTREVLTIDYDDLIGKAARLMIE
                     NHVHSLLVIKYGKPTYMVSTYDLIRVSYEEAFNEGNADMLRTPVESLVKGQNLVSVTT
                     DTSLLDALALFVQYSIHSLPVIDDGNVMGILTLMDLAKWYKKTHE"
     gene            39372..39650
                     /locus_tag="PLF66_03750"
     CDS             39372..39650
                     /locus_tag="PLF66_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HMZ63184.1"
                     /translation="MNNEESFWSDVSDSDIARERKKAKELKQTAWWKQKRSNGICYYC
                     KKKFPVEDLTMDHLIPLIRGGKSIKSNLVPACKECNNKKKHNLPFELG"
     gene            complement(39642..40931)
                     /locus_tag="PLF66_03755"
     CDS             complement(39642..40931)
                     /locus_tag="PLF66_03755"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HMZ63185.1"
                     /translation="MNEDKIPVGINPENLKPFKIITAEDSMIDRSLLRMHLQSEKFDI
                     IYESTTGEDLLFYLQESLNKPDMICVDVNMAAGNGIEIIQKIRSSYKNIKIAVVSAAS
                     DKNIIQSLIQLKIDTFIKKPYNRMQIVQKLSQALGRKEDTSVTKDQIPAVNINFANLV
                     IPPLPAVAVKITTFDIDNPSGGSEELEKIISPDKSITMDIMKIANSSFYGRSGKVHTL
                     KDAITLLGTKTVKNLVLLQSNKQVSKKLKGDIFQKNLQELPILSALIAHDISIPLGMK
                     SFKDDLFLSALLKKIGMTILALTFTERYEQMIKQATSESKDIYALEKEAFSFDHIDVG
                     SKVFKIWNMPKGLQDAVAHQNFTAEDMGKVSHVDRITRLAGLLAYQMVGYELREEEKK
                     LEENIFQAYNATDKVKESFQKEYYDLIKDHPFFEMLA"
     gene            complement(40947..41144)
                     /locus_tag="PLF66_03760"
     CDS             complement(40947..41144)
                     /locus_tag="PLF66_03760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63186.1"
                     /translation="MGLFDKFKQFLKGESVQSSKNNSKTITFENDNGVICYKIDGVKY
                     NSLDEIPDPNDREQARRITFR"
     gene            complement(41158..42114)
                     /locus_tag="PLF66_03765"
     CDS             complement(41158..42114)
                     /locus_tag="PLF66_03765"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HMZ63187.1"
                     /translation="MRNSKIGIILFIAIVSLADCRFSFYENKPIPNLDLQGHRGARGL
                     FPENTLPAFKAAMDLKMTTIELDTVLTKDKKLIIHHDNFINPELCKWKSGKPVLPEPI
                     GNFTALELKELDCGSLKNKNFPEQKTIPGTQLSTLAEFLQFVKKYETDNKITTPFFYN
                     IETKLDESNYTKQDVLEYATTIVHTIEEADVVKYATVQSFVLDVLPEVKKLNPQIQTS
                     ALFAPSKLEFFLLSLGFFAPSQKIIDRAKELKVEIISPFYKYVDKKFINICRQGQIKV
                     IPWTVNDKETMLKLIDDGVDGIISDYPDRLISVVTSNAIIKK"
     gene            complement(42593..43444)
                     /locus_tag="PLF66_03770"
     CDS             complement(42593..43444)
                     /locus_tag="PLF66_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016758692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63188.1"
                     /translation="MPFLVSGNLNRIKQSSLVKFQKNFGLILSANQFEKQQYQMIQSA
                     LRTKCNFVQVFSSLAKHEVISLVFLISQFGDALKTEIPPEYAYFDEIPYILEWNDGAY
                     MIPMEVLDFLSHEKIFKDQNYLFALLPLIPSKEKKAWIKWIGTDYEGDSDRDLNHELY
                     MRSRILQKPFKGKSYIHENEFFLEQLWPVGKNKIVDWYYKGLTTFYFAMQELARVEHD
                     PFFVHVLEEIKSGKYVLKKVPQEFGATEKFKLVSTVEGKSVQLRETLFNWEFEKKKQA
                     DYLFYNI"
     gene            complement(43451..44713)
                     /gene="purD"
                     /locus_tag="PLF66_03775"
     CDS             complement(43451..44713)
                     /gene="purD"
                     /locus_tag="PLF66_03775"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011069773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HMZ63189.1"
                     /translation="MKVLLIGSGGRESALAYKIRQSKLLKELKVFPGNGGFPQSEILP
                     KNSLNLKDKNSTVDFIRKEKFDLVIVGPEEPLVDGIADWLEEVGILCFGPSMYCAQLE
                     GSKDFAKNLMREFNIPTAKYQTFTDYKLATEYVDTHSLPVVIKADGLAAGKGVTVCQN
                     KDEALNALKEIFLDKRFGASGSRVVIEEFMQGEEASIFALSDGEDFIMLPALQDHKRA
                     YDGDLGPNTGGMGAYCPAPIATSRVYDQVRTEVFKPMIEGLKKKGHPYKGLLYAGLMI
                     QDQYAKVVEFNCRFGDPETQALMLMIEDDLLELFYNAAKGSLGKKEIKIRSGSSCVVV
                     LAATGYPNDYIKDIPLNLKTGSQDIQIFHAGTTYKNDILVSSGGRVLGVSSYADNLRN
                     AVDKAYQYLKSNPQQNTFYRSDIGFRAL"
     gene            complement(44716..47367)
                     /locus_tag="PLF66_03780"
     CDS             complement(44716..47367)
                     /locus_tag="PLF66_03780"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008593262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HMZ63190.1"
                     /translation="MKKEISDRYEPQAVEKKWIEYWEKNNSFKVDYSRKENFSIVIPP
                     PNVTGTLHIGHALNHTIQDIIIRIERKKGKAALWVPGMDHAGIATQVVVEKQLAAANK
                     KRTDFTRQEFVDKVWEWKAKSGGQITHQQKMLGESVDWSKERFTFDTGLSAAVTKVFK
                     SLYEEGLIYRGERIINWCPVTKTAISDIEVEFRETKGHLYHLRYPIKGTSDYIVVATT
                     RPETMLGDVAVCAHPDDKRYANKKGVTLILPLMNREIPLLFDTFVDKEFGSGLVKITP
                     AHDANDYEAGKRLGLTPVNIMNPDATLNENAGDYRGLDRFAARKKIIEDLQKLNLVEK
                     IEDYTHSIGHNQRGGAIIEPYLSTQWFVKIKPLAEKAMDVVRNGQIEIIPKMWEKTYF
                     EWMENIKDWCISRQLWWGHRIPAYYSPDGSMVVAESLEDAYQAFKSKNPSLKKEEISQ
                     DEDVLDTWFSSGLWPFSVFGWPEKTEDWKRFYPTSILVTGFDIIFFWVARMIMFGLKF
                     NDGVIPFKKVLIHGLVRDKHGKKFSKSLGNTIDPLDMMSKYGTDSFRFFLAATLPEGK
                     DILFDESRLDGYRSFCNKIWNSSRFILMNLPEDFSLKKISGSDLEASDAWILNRFNQC
                     LADYEKAYSEYHFYEMAASIYDFIWGDLCDWYIELVKPRIYGKSGEKSLEVARQTLVH
                     VLLSALNLLHPFMPFITEEIHSAFAEKDLTNSDWSKPFSDIDSKKVEKLSILIEIVSK
                     VRVVRADQNIPPDKKCKVIVKSSDLLAKEIVEEERLAIMQLSRAEELTYNAEYSVEKS
                     DSVAAFSKGEIILPLSGMIDFKKEQERLTKEKTSLESEMEKVNRKLTNPEFISKAKPE
                     VIAKEKEKIEGLKSKLETVIKSLEKLG"
     gene            complement(47404..47658)
                     /locus_tag="PLF66_03785"
     CDS             complement(47404..47658)
                     /locus_tag="PLF66_03785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63191.1"
                     /translation="MGDELSGFVINTLEANLPIIKKYVVEKVGPAAREVIKNDEQMKV
                     IFRTVYNLMPFAVRMAVTENDFMMFAMKNKHVLLDLTENT"
     gene            complement(47753..49357)
                     /locus_tag="PLF66_03790"
     CDS             complement(47753..49357)
                     /locus_tag="PLF66_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010573110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HMZ63192.1"
                     /translation="MGIPEINNKIITPKKHKDIISENKIKSGKWELSADVIIIGSGAG
                     GSVAAAELSKNGWNVVLIEEGSYFTPAQFNGDEFLSNARLYRDAGFVLSEEQTLNIVQ
                     GRTLGGSTTVNWQTSLYPPIYVTDEWEKRFGLKDYSKEKMNPYIEEIHARLGVHQVPE
                     RLINKNNNVLRRGGEQLGLHPEVLPNNNRGCHGLGRCGLGCPINAKQSMFLTFIPDAL
                     EYGARIITNMRAVKIKDGKVKTVIAEFTPDPYDSYPDDLIREMEIKAPVVILSAGAIE
                     GPALLQRSGLGNNWVGRNFKVHPTSTIFAHFEEEINMFSGPPQSIVIKDGHNQDNSGY
                     GFWLETAPYRPAIATSLMPFYGKQQFEVMQNYKNLQAGIVLVRDGADGETNARIEWKM
                     GKRKVYFEITPTDGRNLLRGLKMLAEVQTAAGAKELIFPFTRMKTPFKVDRKSNFDWI
                     LKEPITTGSINIASAHPHGSIQAADSPEKGAVDPNFELYGHKNIFVMDASVIPTGLSV
                     NPQITTMSLTLRASRTLASEKTKRVG"
     gene            complement(49359..49862)
                     /locus_tag="PLF66_03795"
     CDS             complement(49359..49862)
                     /locus_tag="PLF66_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010573111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63193.1"
                     /translation="MNFLKEDIPRSEFLRLAIKAFAITALSFEGVNCGNSLSTPTLKG
                     ISSEEYFNMRSMQEIFLEGNPIPNFDLGKALDDYIYGHPSPIPTKDLIHELAGAPSSI
                     LAAILLDFSLTPLVKLNKEEREKRLLSWKNSNSQMKRGLFSILRQTCMFLLASNKEYQ
                     KAIGYEG"
     gene            complement(49859..50665)
                     /locus_tag="PLF66_03800"
     CDS             complement(49859..50665)
                     /locus_tag="PLF66_03800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63194.1"
                     /translation="MKKLFLLFVCFFCVSCFDIFHYIEVTDKKFRAEYRISFSKALFA
                     MGGVNSPVANLDIDDILNKPELKKLDGTFSGAKKETDSSLILHLKYIGSESSLTKRVE
                     TDSFPMIPFKDENGQFIFIFRQNKTNPPAGTQENQMAEAIMSGPVYRLLVGGELKPKK
                     AVFKKKNQESSIDLEIIPLGTQYLIEFPISYILKETGAFVVSFTGEMKLETVNKILNE
                     EPKEASEQPKSTKDSKTKPKSKTKPKEKDKEKEKDKDKDKDKDKEQESSL"
     gene            51070..51651
                     /locus_tag="PLF66_03805"
     CDS             51070..51651
                     /locus_tag="PLF66_03805"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002974725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HMZ63195.1"
                     /translation="MNSNKKIFRCNWTGDDSLMIEYHDREWGVPVHDDKKHFEFLILD
                     AFQAGLSWRTILHRREGFRKAFADFEVEKVARFREKKLLKLMQDESIIRNRLKIYGSV
                     QNAKSFIQIQKEFGSFDKYIWQFTNYKTIINHWESMKEIPAKTKESDTMSKDLKKRGF
                     TFVGSTICYAYMQAAGMVNDHLKNCFRHKQVKK"
     gene            complement(51641..52963)
                     /locus_tag="PLF66_03810"
     CDS             complement(51641..52963)
                     /locus_tag="PLF66_03810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63196.1"
                     /translation="MKFLFLFSLVLFFSLNLFSNSILLKSGTKIDVKSIEKETEEDLI
                     VTTIDGFIIKIQKDKIESMNLSNKDSLKEEKPPLDPKNSKEDLVNKENSSVPTAKDYK
                     IFFSQSMNNDANIRGASIFGERLNRRDNTPYNSFAQAWVLATAVQVLAPLKGLRMQLI
                     SANAVSHRNNVDSDGRLFQNGPGGEDVTNKSLNDLQSGKLTFDPNQTKRHPERNGLKD
                     FVIGGIYYDWEMGSFGGFTTGLFYVNNTDPYRKNTFTEINMAWKLPFFRELLNPTLNI
                     FNRISSETSTFNNLNNGSTYLSFGGNHDFRKKEYFRFSISSQLGYQYNNNNTDRRSGF
                     SDLSSKIQFFLGQYFLALNHVYRPDVYLWDTDRNFPKIGTYGDINANDGKTVDPSKVN
                     GPLNQFVADQIKANSPNDIVARALTENYQQQKIVRNIFFISFGYTLIF"
     gene            complement(52982..53689)
                     /locus_tag="PLF66_03815"
     CDS             complement(52982..53689)
                     /locus_tag="PLF66_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HMZ63197.1"
                     /translation="MKKIIHKANTRGVADFGWLHSNHTFSFSSYYNPNRMQFGALRVL
                     NDDIVQPAMGFGTHGHENMEIVSIPITGELAHRDSTGNAKVIYPGEVQIMSAGTGIRH
                     SEFNNSEKDLVNFLQIWILPKEIGIKPRYDQKFFDREKRINSIQTVVSPEPSEDAVWI
                     NQDSYFSLSTLENGKTIEYTNHISDNGVYVFIINGSVKIDGEEFSKRDGIGISDTKSF
                     SITALEDSEILLIEVPI"
     gene            complement(53774..54298)
                     /locus_tag="PLF66_03820"
     CDS             complement(53774..54298)
                     /locus_tag="PLF66_03820"
                     /inference="COORDINATES: protein motif:HMM:NF019542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63198.1"
                     /translation="MAEIINLDEFKKNKKKKNPLEQEKQSKISFSILKVTLSDKLGGG
                     IREKSYRVLSIPNKQTLSMLAQFILTSFDFELDHEYGYFDNIENWIQSQEIYLMDGDA
                     SFRSTGYVTKVTVGEVFNVPDKKMLFLFDYGDEWSFIIELIRTEVPAGNVKFTPKVIE
                     KKGNPPPQYPDFEA"
     gene            54722..56146
                     /locus_tag="PLF66_03825"
     CDS             54722..56146
                     /locus_tag="PLF66_03825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63199.1"
                     /translation="MIRIHLVLVFIICFTALPILGDNFQNINGFFGERATGLAGSYTA
                     ISDDVSGTYYNPAGLAFSQNSYYSINASSYNEVKVNHKNLFGPGQNYNRTSRNYLPNF
                     IGFTRKDGRFTYGFSVLTAVNQSFDQSDRITLPIYRRNLSQLDISYTEEKFRLYAGPS
                     VAFALTEKFSIGLTTYYMYDSNRKIVNQNENQFNENVTTVTEQNRRRTSGLMPVLGFQ
                     YMLTEKLSLGLSIRRIFVTGGNVSYRTNVTTAGRTGGPSSIVIQSTENGGAKVDGFNP
                     VLLSPSSYQVPQTTEVRFGWAYFINRKFTLASDIIYTSGYAIQIADNSYNTRTGTFTI
                     SDPYQNDLKRNHTVNYSIGLEYFIFDNFVIRLGHFTNRSNNDKVNWTEAAWYAAYRQL
                     NQANTDVSIRNYFNYSMPEKREEYINLLGYTLGFGVETARNSFSISIMIQGGKGIGTI
                     DRNQLPSTSTYRESTAYLSGSTRY"
     gene            complement(56150..56770)
                     /locus_tag="PLF66_03830"
     CDS             complement(56150..56770)
                     /locus_tag="PLF66_03830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63200.1"
                     /translation="MFGFIMESFDLNSVLILFVALGGLVYLTAKIFRKDTRLKTPKTI
                     PSTASSTDFSPFESNDTDSSESDDMEIEEEEIILKGDMSKLEEFVDKLILLPKDTLSD
                     IQISKSEDDDLLITIEKDETNGMMIEGYYPEEVTSLSYSDWLNATKQVIEGFQLKIES
                     FNEKDQSIRIILEGKSSHEIKEITERLLTQGYLNKLGNSFLVEYFY"
     gene            complement(56811..57224)
                     /locus_tag="PLF66_03835"
     CDS             complement(56811..57224)
                     /locus_tag="PLF66_03835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63201.1"
                     /translation="MRYIEFYLYSSGSTNYTITATNPFGYSITNLTIASAYCVGGFQF
                     GGVCYRLANVGFDCQSTCSSYGNLGVDSISMSAANCQTVLNTLLGSVPAGGYVQWAGS
                     FPCLYETISPFRGIYSTVGFNPNYSNYRAACPCIN"
     gene            58116..59282
                     /locus_tag="PLF66_03840"
     CDS             58116..59282
                     /locus_tag="PLF66_03840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63202.1"
                     /translation="MNIRILTLIIFLSFGVSLSAQDKKNPFPLPTSLSDLEGLWEVNK
                     IEQPYGSTDTSAVQQWLGKKVSVSQSKIQLLDEFELYSKSNFVGKECDTKGGVVRPIT
                     FQKYSKFDANNKDSLTIKADEKLALGEFYCKTTPFAEFLYVPKQESIALYTPTPGAAN
                     ASLSSGFFVYLKRISKTPITTLSVQSGEIAPASFNFTDKAVDAIKELEAFRDYLYLDG
                     NKNYIFGYGHLVAPNPQLVRDGFEHPQEVKDEIQRFKTKYGTGSGDGTATQQEKNEAV
                     DMYLRKDLDIIIGNMKKFITVPLTQNKIDAIVIYLFWRGSYANDTTTNGKMILEFYQL
                     VNAKNDAAVANFMRTRLKKDVDGKDIPFSPGHPDRHNKTATLYTDGIYANPWLW"
     gene            59384..60055
                     /locus_tag="PLF66_03845"
     CDS             59384..60055
                     /locus_tag="PLF66_03845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63203.1"
                     /translation="MNPLHLTLILFFLSFSLFGQTFPSDENRSIKNLTQAHVVPTTVD
                     LTYSEKKIIALVNGSKQLEYKLTGETINTAKEFGNMVLVITNKNLIAASTNANEFARQ
                     PLYGDTNISFTISNCIVFVVSNKKIYSYTVSSSEWRTIDLTGEIVRTHAAFDKTGALI
                     TNKRIISYSEYIKEFHFYKIDDFTPINSFNVLDNKIVFESNASPRKTIIYRAQTGEYS
                     VVNFN"
     gene            60096..61928
                     /locus_tag="PLF66_03850"
     CDS             60096..61928
                     /locus_tag="PLF66_03850"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HMZ63204.1"
                     /translation="MRFKKTFFILSILIISISAYTLLSQTNSPASNSPESAKPIEAGT
                     KYPEYIWPIFGDKLVTGSFGEFRYYHFHMGQDFATEAKNGLPLLAMTDAKVIKLQSFR
                     YSIGNAIILLHDDGFMSRYGHMSGFAENIWAAIQDKDVLEKRKRRQDFEYTLTASEQV
                     RVKKGDVIGYSGDTGIGPSHLHLEIFKDNIYYNPADFGLNYFAFGGINVYAVDIVPEN
                     NKSFINGKNATYTLRFKLDKDNNLVPSKNEILKIKGDVSLSLYGSESSGRSNKIGFQK
                     ITVGLNGSELQEVNFTKISSFHTFRSCFVLDNYRSRMNGRPFKYFTHTKEGNTLLGFK
                     NQEVGAGIVRSGSFKDGENEISLGVYGISKLPAIIKIKAVKDNNEYPDGEIKKINLSH
                     DSYITVSTPDKKGEAFFPAYSIFTQANVSIEKSKNQKISEKDMTLESEVYTVEPDYKE
                     FNLGYDLYIKLDKDINPEKFGLYQLSNNGKVIKNFPGIYYNTTERFFRARIKVTGNFA
                     ILSDYAKPTLRIYRYKDNHVFKGKDFKIYMVASDHGTGIPESNFQVKVDEKEAYTDLD
                     PETGLREIFYPDSMKEVGKHVITATAKDRANNVADPLVFHYEVK"
     gene            62415..65498
                     /locus_tag="PLF66_03855"
     CDS             62415..65498
                     /locus_tag="PLF66_03855"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HMZ63205.1"
                     /translation="METIFHQLPVLPTNPTAWEYFWNQMSHGFPTGLTVVIGFFIAML
                     GFWSAYNDKEKRDYYISFSLLVLGLTGFVFLYFVRFVFWDTERILFLNLFIYPIFRLV
                     TFGLTYFAMVSTQKTKFMKFLLLITVFIEVKILYHTFLGEGFTGAFYENELGKFPIIT
                     KGRGVLVLLPAIIGLYDIIRISYFIFLKKSKIHISIVIAVIGFFILNINSALLATGDS
                     KLPLVVFSFVPMFLIAYGVFHSDFLNLNELLFKKNLLFYIFSVSISILFLFVSILVAY
                     YLNPEHGFHYRNGYFLISLFSFVTTICLSAYIAGNKPDDKFVMFVALNIFIQGFLAFL
                     VMIRGLYLPLIIEHRLNQILFLFFAFSSSIGFRILYLGYNKPIPKLSYLIDISNLICS
                     LFALSPYFFNGYYEYNWGRYPSAGFFLQISQTIIVLVLLKVLYDWIQFRKSNKNSLVD
                     WIMICSIIGAFLSIGSIPATRGIEFYPLINFQFIVTFLYAFAIIKFNTLPIQGESTAL
                     TNRISFYAIFVIPIILLFYSSNLSKEIQGTQIVYHLSLIGSSALVSFFAFTFILLRPI
                     ARKLDEEKDKIEEARKQADKEKAIAILARLETEKQKKETEELNKLVKSLNEELDLKVI
                     MEKVHKYVKENFGIEYYGLFTVSQNNTYIRPLDMYFADFVMEEDRSKIFNFKIPIKEE
                     RSGHAFPIKSKRPFYLPKMRTLAATKEEIYFSEKCRIQSFILIPLFLNNEPIGTLDLY
                     NSSKRMNLSKDDITRLSILGEQLAGIIYGSNLYKQVEEKTVELNRSLQLIKKDLNFSS
                     KIQKKLLPRESLQISGLNISSFYAPMDEVGGDFFDITVHENKIRILIADATGHGVQAA
                     LITMLIKSEFETVKHSSSSPSNLLNELNSSFCSKYSNLETLFSCFIADIDPIERKLEY
                     ASAGHPAQLLASNHEIIELRNTGRIIGLKKDTNYQNIKIDFPSGSKLFLFTDGLYEEI
                     NLKEEEFGEERLKQNLFQYKENHSDLIIENVISDLKNFLGESEIHDDITFVIAEW"
     gene            complement(66169..66519)
                     /locus_tag="PLF66_03860"
     CDS             complement(66169..66519)
                     /locus_tag="PLF66_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010570704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HMZ63206.1"
                     /translation="MKKLLVSIVLQVLVVMFLFPMIDAGFRVKGDFKDALIIVIVFIV
                     LNFAARKLILIFTLGIGALVYYLTLGVAGLLLNAVIIYAVKGLFPELISVPNFTAAFL
                     GGLFLAIANYFGGR"
     gene            66648..67634
                     /locus_tag="PLF66_03865"
     CDS             66648..67634
                     /locus_tag="PLF66_03865"
                     /inference="COORDINATES: protein motif:HMM:NF017770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF898 family protein"
                     /protein_id="HMZ63207.1"
                     /translation="MTESNIANSEGSSRFSYTAKGGEYFGLAIKTFFLTIFTLGIYWF
                     WGRVKIIKFYYNNTKLQGASFDYHATGKEKFIGFLKGVGILLSFGLINFLISYIITKI
                     ANASIANTISIFVTYVVIFTALPFVIILSLRFRLSRTSFNGIRFQFLGDPKEFLILFL
                     KSLGLTIITLGFYYPWFLVKITTYIAQNSLYGNHAFDLRLKAEELFIIFLKGFFFTLF
                     TFGIYFFWFRANVFNFFWNNYYFQNGKTSSNLTGGKLFLTAIKSIGLMIITFGFGFAW
                     VIIMNQRVFIESISFSAPIDFDAISNIPLKPGSATLDSLGGDALDSFGSIVG"
     gene            complement(67847..68836)
                     /locus_tag="PLF66_03870"
     CDS             complement(67847..68836)
                     /locus_tag="PLF66_03870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63208.1"
                     /translation="MAFRFFLFLIYFILLIHSIGAESKRKDALHNRKNLLSKITNENG
                     KIFYLEKQISLSEYEEKKKKEIATFKEDLKSIYKLDFKVRYTKYYNIAYQCTEKEMDT
                     VEHKLKVFFDDTYARYFIYEPRFTLRIIFFKNKEQFSKKMGYDSYGVYFPESPDYFTS
                     TERTLYTYYNSGPGTLWHEMVHSFVDLNTETDVQQWFNEGFASFYEMGSVQNNKFIEG
                     YTNWRLPELQGVIRNKKMKPLKKFLLNSEMNEDYGYSAARFLFVYLWTENKIIPFVKS
                     YIYDLSPNYQGKELGQKAIEKLEELLGKPIDQIEEDYIKLALKSKTYEKLKQK"
     gene            complement(68839..70269)
                     /locus_tag="PLF66_03875"
     CDS             complement(68839..70269)
                     /locus_tag="PLF66_03875"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012389915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HMZ63209.1"
                     /translation="MKILHTTAEFFPYIKAGGLSDMLSSLSDYQSKENEVFIALPIIQ
                     NLNQAPEFTGREFPCIDESIAYHSDACAILKKSKFREAIDGNKKLYFFDSPIFQNLQT
                     IYANANEHFNFAVFSYACYHLAVELNVDVVHSHDWHTAIVTILCNTQPVRKPTCFTIH
                     NLAYQGDHPVDMCRFLRIDPFYLNLDLFDHLHKSNFMKAALVVSQEITTVSHGYRDEI
                     LKEPMGNSLSWILHQRQDSLTGILNGINDKEWNPALDKKIYKNYTFETIEKGKLANKL
                     ALYQEYKLHVDLHRPLVGLIGRLTHQKGFDTFLQSFYERWRMPFYYFVLGSGDKQIEG
                     ALFHESHHSNARIFFYKGFDEALARKIEAAADFFLMPSLFEPCGLNQMYSHAYGTVPI
                     VSRVGGLKDSVHETWDRNKSTGFVFEPGVEHSLSYALDRALTLYHDKEELNFKRQVIM
                     KLDWSWKKGSSEYQALYNRAIQKVKG"
     gene            70427..71242
                     /locus_tag="PLF66_03880"
     CDS             70427..71242
                     /locus_tag="PLF66_03880"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HMZ63210.1"
                     /translation="MSKTFIVTGASGGIGIQIVKKLLEEGAIVVATDIDLKSLEELKT
                     EFSNYKEKLLTEILDVRKPELWEKVISLAKSQSAPFYCLINSAGVLMPGYIINTTPKD
                     IDFHIDINAKGIMLGSQLAAKYFVERKAGHIINIASLAGLSPVPGIALYSASKFAVRG
                     FTLALAMELEKYNVNVTCICPDAVTTPMLDLQKDYEEAALTFSGSKPLSAEEVADVVL
                     DTIGSKKMEVTIGYSRGFQAKLAGMFPALASKIVNVMRLKGIENQEKFKRDLE"
BASE COUNT        23642 a        12762 c        11199 g        23959 t
ORIGIN      
        1 agaacgtgcc gtgaattcag atgaggaggc cttttataaa aactttcagt ggctattata
       61 cgagaaacgt tttgttgtga tcatcccagc tagcgcaaaa ctagatagct ctttaacgtt
      121 tcaagaagca ttatttatca tagccattgg aaatggtctt aaaggtatga tgagtctgaa
      181 gccatattgc agtggttcga ctgcttctac ctgtggaggc gctagcaatg gaacatggca
      241 aaaaaacaca tcggtcagcc tgagggacaa cactgctagc tataaagact ttactgcatc
      301 caaattttct agtgtagcag gagattctgt tgtttatgtg gaaggatggg gatatggagc
      361 cgctggaaat ttaccatttc aagaagcttt tgttttacag tccaatgctg tatggccatt
      421 actcatacca aatccaagtc aagtttacgg gcttattcct cctgtaattt cgtttaactt
      481 tcctgtttta gaaagacttg gttttactac cagtgcaact gtaataccta gttcgacttc
      541 tacttattgg gaacaaagaa ataagctttt acctctgatc gcagctcttg ctaaaacgct
      601 agatgaccaa gcagaccaaa cagtagctgg taaaaatcct tttaccctcc tgactgattt
      661 aagtaaaatt ctctcaagac catatgtatt tataaacact gacaatgtaa ttttgccgaa
      721 tcctcaatac actgttaaac ctaccatcgt agatatccgc cttactgatg ataatccaat
      781 tcgaagtgct acagcgacac cagccgaata tttgccttct tctattatct tttcaccagt
      841 atcattttta gttgagagcc aaagaaaatt tcaagatggt gcgttagtat tagcagctca
      901 aactgatttt attacaaatt tagtccagat gtttgttgca ttaggagatt ctagtaaaaa
      961 atcaggtaga acaaaaatct gggcagcggt gcaaaagcta cttggagaaa ttaaaatcaa
     1021 ctcagatgga gcaacttctt caatacaata caatttgcaa caatttgtaa tcgataaagt
     1081 tgctgttctt gccgcgtatc ccgatacaag atctaccaat gtaaccgata gttcctggaa
     1141 tggagtaaat gactctgtag catttgcaag agattatttt tctaaaacat ctggctactc
     1201 aattgtaaaa aatatagatt tcattcttgg attgataatc gatgtgactc ctactagctc
     1261 tgaggtgaca tctctattta atatgctgga ttctcttctc acaaaagagt ctaacggtta
     1321 ttacaatctg acgaatatat tgactcttta tgtcccacca gtcttaattc agacagcgcc
     1381 atacggtaga aatttatttg gggtttttta ttcacttgct atccctggtg gctttgttga
     1441 ctatatggaa aataaaatga cttttatgcc atacagcatt gctaatttta tatcggacac
     1501 agaaagactt ttaaactcga gtatgattac aactacctca aatacgccag attctcttct
     1561 ttattctagc ggtgtcttaa ttcgtgtctt tgctgatatt taccaatacg gaaagaaact
     1621 tgaaaaacag ggattgtcat ttgctgataa tttaaatgta aatgaaagca cttctgtgtt
     1681 tcaaagattg aatataatct ttagtataaa ataagggttt acaaaatatt agtatgcttg
     1741 ggataataga ataaactaaa agaataaatt aatttactaa aaataagtta atgaggattt
     1801 gaaaatgaaa accattgcga aaaaaatatc tctctctatc tccatcttat tctttttgct
     1861 cttccttgga tgtaatccag cggcaggcgg taaaaagaaa aatgatatct ctaacttaat
     1921 ccctctcgcc ttgacaggtg gtggaagtgg atacagtgag cctaacgatg gtggtgttgc
     1981 tccttctaat tacaatagca ccgatactca aatcaatctt ccaaatggaa ctactgtaac
     2041 tggaaactgt gttcccgcag aaggacaggc tgctagtcta tgtttaattt cttcggataa
     2101 cgttgatcgc tacaaaagtt tagaaattat tttcgcaagt ggtatggata aaaaatctgt
     2161 aaagacagcc ttttcactcg ttagcttaga ctcaggtgca ttaccatcac ctggtggagc
     2221 agatttagat gacgcagacg tttcagacaa aggtggaaga tttacttggc tgagtggacg
     2281 tagacttata tttgatccat ataaagaatt aaaaccaaac gaaacctatt ctttaacaat
     2341 ttccaccgat gctaaaaaca cagcgggcac tccgatttca tctcaatata ctacaacgtt
     2401 taaaacggaa catgattttc aaattacaaa tctaattaat ggaataacgt taggcggaaa
     2461 taatgatgta acatttaaca aatcagctcc gcttcttttg attagtgcct ttacaaatcc
     2521 tcaaggtgct tttattaaca ttgctaaaat tactcttttc agactaggaa cacagaatgg
     2581 atatgatgtt tgcgtttcta gttgctctgg tcttttaact tcatttaatt tgaatacatc
     2641 tgctgttcca ccggttgaag gtggaaacac atattactac caaatccaaa ccaaagacaa
     2701 taagacctac aaaagatttt ttgcattcaa ctggggagac gttagttctg catcaggact
     2761 gatcgcaaat gcagcaacag gagttctaga tgaaggtatt atgatgaagc ttctagggca
     2821 agtcgttgaa agatttgtaa aaactgattt caaagtaaaa gaaggaactc aaaataaaac
     2881 cttcaacgac tttatgggac ttccgacgac ttccaccaaa agaacgagtc attgtataaa
     2941 ctatggtagc tttagtttca tcagaaccta cggtgattct ggctctgcgc ttggggatgg
     3001 atactgcggt ggatcaggtg aaaatccagg agcatttttt ggagcttaca atggtatttt
     3061 atcttggggg agtgatgtat tttttgatat ggatgtttat atttcaggag ctagtattcc
     3121 aggtttaaca aatggagttc agactgttga agctgctatg aatgccacta gcaccgataa
     3181 cctccaaatt gcattaaaag gtagaaaagc atttattaat ttagaagttg ttgcaaaaaa
     3241 tagatctgga cttggatgtg ttggcatctg tgcaattccg agtggtagca gatttcattt
     3301 taaaacaaca gttgagctga accaatctcc aactccaaca tcccttagaa cagccattac
     3361 caacaataat atctctgtta acaatggagt tttaagtctt ggtataaaac catttactag
     3421 agggaatgta aatgatccta actttacaat ggcagaatgg gactctaatc ttgccgttgg
     3481 tggaatgact ctggtgaatt cagatagttg ggtgtcagaa cttctaagct ttatcacgaa
     3541 tatgatcgca gatggtcttg ttccacaggt tcgtcccaaa attgttcacg caatgttaga
     3601 agacgttgtg gaaaaagtag cacctaacgt tttaaatgca gtcttaggat ctcttcaaaa
     3661 tccaggaatc aatttaaatt taccaaatta cttgcctgct ccccttgcaa atttcccatt
     3721 aaacgttgca atgaaattac aaaatgatgc acaagttaga gtgataggag caaacaaagg
     3781 tttagtctct tctgtagact taggaatcac atatgcggga ttagttcctg ttggtggata
     3841 tagagttcag ggtggacaac aaggtatagt atcttttaaa cctccaggag cgttagactc
     3901 tacctatcca ttctctcaaa tttcagtcaa tccagggatg ctgctttctc ttcatacaga
     3961 ctctgttaca caagcagcct atcatatgtg gagagcaaga gcaatcgatt tgaatatgga
     4021 tgaaacattc atagatacga taaaccaata tgcaggcaca agcgacttat ttaaactgac
     4081 caagacattg ctaaaagctt caccaattat ctcgattcta gctccaggaa gatcaaccct
     4141 gattggggtt aagaatggac cttttactcg tctaccgcct atcggtgacc aagatccaat
     4201 aacgtttgct ttagaaccaa ttcaagcccc tgttataaaa ctagttccac cggttggaat
     4261 tggaattcca caagtgcgtg caaatttaac agatttgcaa atggcgattg ttggaaaaag
     4321 aacaagttct gcagaatatc cttgttcagc aggtccaatt acagctagag atacaggaac
     4381 aagctgcttc tatacaatca gcactgttcg tgtaagtatt gatgcaatgg caagctttgg
     4441 atttaaaaca ttttccaatc caacatcaaa cccatcttta aacaacataa atgctatata
     4501 tgttcagatt gatccaaata caaattttta ctattctatc gatgttagag agggcaatac
     4561 ggtgaaccct tatggactcg atccggaagc gattcgaagt gttgtggatc cattggttaa
     4621 atcccttgtg gttcctttgg taaataatat cctcaaagag atacctcttc ctcctaaatt
     4681 aagctttcct gcactcgttg ataaaaatgc aggtgcaagt ggtggaacca attgtcagtt
     4741 aaatgttcag accacagcta ttaacttaag cactatcgca acaccatcta ctgagtctta
     4801 tgtattaaat aaagtgcaac cacttggagc gtatgctaca aatccaggcg cacttgtcga
     4861 gtgcccataa gattttaaac ttgatatcag tctagaattg caaattttgt aaataaagat
     4921 gcaattctat gactgagctt cgtaaatatt tattagccca caactatgaa aagattattc
     4981 ccaaagtccg ggaactaatt tccgaaaaca aaagaaactt taagaaacat gaaataaagg
     5041 aatggtgcgc cattcgatgg agaaatcttt ttatcaatga agccgcaaaa attccaaaag
     5101 ccgtatccgc agcaagtcat acaaaacttt ttggtggcaa ggatcataga agcgataaac
     5161 aaaaagttgc agaaagattt ttaaaagagg aaggaccaaa cgaatggaga actgaaatgc
     5221 cagacgtgga aatagatgag ttgcggcata caacgcttat cttctgtcct ggttttttaa
     5281 atggtctttt tcctgtaatg gcattcgaag atgcatttcc gcttttagag aaaaattata
     5341 aattacgagt gattcaatcc gactctcatc caatgagaag ttgtgaagga aatacgattg
     5401 atattttaaa tgcaattgaa aaaggcattg gtctaaatgc aaaggctgaa ctgatcgatg
     5461 agagacatgc agtcccacca aaagatattt ttataatagc ttatagcaag ggaatgccag
     5521 atcttttaca tcttttagta aaaagaccag atttaaaaaa aagaattaag tgcattttta
     5581 actgggcagg tgctccgggt ggctcctatc ttgcgaatac tctttatgat tctatcaagg
     5641 atataaattt taatattaaa gatgaattcg ataatttaat taaactgatt tcaccaatca
     5701 ttcatgttcc gcacaaaata agaaggctct ccgaatataa cgttaaagct gcaattctag
     5761 atttaacaac tgaatttaga aaagaatttc tgcgagataa tttaaaaaaa attgatagtc
     5821 tcaatattcc tatctttaat attacagcat ctaccacaat tacagaagtc ccttactttc
     5881 aattacaagg agtaaatgaa ttaaataagt atgatgcaaa caacgatatg caggtgactc
     5941 aaaaacattc taagcttcat ataccaatgg caactgactt ggctatgtta catggtcatc
     6001 actgggatgt atcctacaac tcctttgtag gaaaattaag ctttggttcc ccaaatttag
     6061 atcacccttt tccaaaagta gctggagcga ttgcaatggt aaagttagcc tatgaattgg
     6121 gactaatcga ataagaaatc ttatttgatt caaattgtta agcaatcatt tcgattcagc
     6181 tatttagcga atccttgcga ctctaattca cttcccattt tatatgactc gaaagttatc
     6241 tcttcatttt taaaaagaat cccaaaagaa taaagtttgt gagttctttg aatgaagaga
     6301 gtatactctt cattccttga taaaaatcat caaaacaaat ataatccctt gcaaaaatac
     6361 agtgtctttg cgggtaatat taaatagccc gtagtaaata aatttacggt tcatttggtc
     6421 taatataaaa agtatattga tctattaatc aaaactagaa acattaactc aaaaaaagat
     6481 accaatttta ttgaattaaa aaaggctcaa aaatgaaaaa aaatattctg atcttattcc
     6541 ctatcctgac ctctctttac tgtttagagg ctaagaaatc accttttgat gcaactaaaa
     6601 atgtaaattc tcttttatca tgggttgcag tgctgagtgg atttggaaac aattcaacta
     6661 catcttccct taacgttgtt tccacatctc cggcggataa cgacacagga gtaaccgcga
     6721 acgcgcaaat agcaatcacc tttaatacaa caatggacaa gactacaatc actacgaata
     6781 cagataacac aacatgttca gggactgtcc aaatctcctt tgataatttc gcgacttgta
     6841 taagaatgag atcctccatc actacaacag ataataaaac ttttatttta aatgccaacc
     6901 caactatggg aacttacgca acacataaga ttagggtaac gacaggcgca acgagtcaaa
     6961 caaaagaaaa actctctgcc gactattctc atacaaatgg cttcacaaca gcaactcctt
     7021 gcactgttta caatgcacag acttgtccta taccgtttag cacaatacct actctaagtt
     7081 tttctttagg aaatggtggt ttagtgattc caattgagag cggaactgcg aaggggaaaa
     7141 tacttgtcct gcaaggggga gggacatcga atacgaatat ttatgatccc gatttaggta
     7201 tttttattac cggtccgaat ttaccatctg tgatcgcaaa tcaagattca ggaacagcat
     7261 tttcgataaa ttccggaact aatgttggaa aggttttaat tgtgcatagc gtttcaaata
     7321 tatcatccat ctatgatcct gtttcaaaca cgatgcaagt tggtccaaac cttacctcta
     7381 gtttagcttg gtcgaataac tttcttgtga attcgggaat aaattctgga aaaacggtta
     7441 ttctctcaca aacagtaact acaaatatct atgatccaat ctcaaataca ttctctgttg
     7501 ggaataactt gaacactgca ttaagtaaca atggatcaaa tctcactgta ataaattctg
     7561 gttctaattc aggtaaggca tttatcgtat ccgctaataa tttaaatact tcagatattt
     7621 atggacttac tgttgacgga cagtatggga ttggcccgac ttttagtgca aatccatttt
     7681 atggttcaca tagtttttta attccatcgg gtatacaaag tggcaatata cttatagcaa
     7741 gaggaaatag ctccacagat acatttatcc taaattcaac ttctttaaca ctctcgactg
     7801 gaccgacatt acctaacttt attgatactc tcgctgatag aggcacgcat agtattttaa
     7861 tttcaagtgg aattaaccaa ggtaatattt ggctattgca tgcaggaaat aatacttcac
     7921 caacttcctt ttacaatcct gcaacaaata cgttttcaac tggtccaact atgccgggat
     7981 taatccgatt aaattcaaaa actgtttacc tagaaacagg cattcatgca ggcaaatatc
     8041 ttacaattta cggatcaaac gtaaaattca ctagcctcta cgatccgatc aaaaatgcac
     8101 tcgatgcgag cagcatacta ccgtcagccg ctggagtagg agtaaaccga attcctatta
     8161 cgacagggac gaatgccggt aaaatgctag ttatcaatgg aggcaataat accagttctc
     8221 tatttgattt tgcgagtggg actttcacag ctggtccaaa cttaacgggg gtaatgggaa
     8281 gaagccccgg tctgtttaca atttatggcg gaacccaaga cggaaagacg ttagtcgttc
     8341 atggtggagg gctaacaacg acttccctct acgatccaat tgcaaataca tttagtgcag
     8401 gtcccgcgct atcgactgcc gcacacaata ttacgaatga gtgtatattc aaagttccaa
     8461 gtggagcaca ggctggaaag tatttgatta ttcatggcaa tgtaagtcaa aatacatccc
     8521 tctatgatcc ttctacaaat acgtttgcag caggtccgac taccggtggg attatcggac
     8581 taggagcaac ttgttttcct attaattccg gggctaatgc tggtaagttt cttgtcattc
     8641 aaggattcaa tagcactccg actagagtgt atgatccatc cacgaattca tttgcagcag
     8701 gaccaaacct tacgattgcc acaacggctg gaacattttc tatgcctatt acaaatggat
     8761 ctaataacgg taaggcgctc gttattattg gaacaggcaa tactacaaat ctgtatgacc
     8821 caactacgca tacattttca gtaggaccaa atttaagtgg aagcgcaagc agtatagcac
     8881 ccagttccta tttgcttctc tcgaatggag cgaataaaga caaatactgg attatgcacg
     8941 gcactacaag ctcaatctac gaccctaata caaacgcaat cagctctggt ccagctattc
     9001 ctgatggcgg cagtttagct ttcaacataa catcaggatt attcaaccaa ggcgttcttc
     9061 taattacagg tagtgggaat ttgacttctg ttttgtatcc gtaaggagaa cacaattcga
     9121 ttcttaatgt cactcccgaa atcccctatc ggaactctgc atactattta gtttgagaga
     9181 cccccgataa aagaactcgg ggggtgacac ttttatctct aaataaaaac agcaggcgat
     9241 acaccaaatc tcttgctgag ttcttttatt tggcgaacgt ttaattctcg tttgccgttc
     9301 aatatttcgg atactactcc ttgacttcct agttgtttca tatccttctg agagagatta
     9361 tgctcttgca ttaaatattt aagcacttca ataggtttag catctatcgg tttaaaattt
     9421 tcttcttcat aggcttccac aagacttcct aacatttcaa gcaatccagc aagaggatgg
     9481 ttttgtttgt ttccaacttc atctattact aaatctaaaa gagaaacaag tttattgtat
     9541 tctttcttgg aatgaggaat tgacaaataa tctttcactt ctttccagcc tgcttggatt
     9601 ttttctaatt ctgcaatcat actttatttc tccttccatt tatttttgtc atattcagag
     9661 tgagtgagaa tatctctaat gtaaactttt tgcctatcgt agtgaatcgc tactattaat
     9721 ctaaaatggt ttccatttac attgaaaaca gtcagatttc caacctgatc tactgtgcga
     9781 aacgtgtgct ttaattcatt gaagttaaaa aaatctgatt cttttataat cttataccag
     9841 ctctttagtg agctttctga atttggataa tctattataa aatcagaaag ttttttccaa
     9901 ctaatgatat gcacatctta aaaacatctc attttgagat ttacgcaagc attttttgta
     9961 aaaaaaaaaa aaaaaaaaaa ttaatgcgct tcttcccaat tatcaccgaa ctttccttgt
    10021 gcgataattg gaacttttaa tttaattgca gattccattt ctttttttgc gagttcataa
    10081 acagtgtctt tttccttttt atgaacttca aaaaccaatt catcatgaac ttgtaaaatc
    10141 agtttcgatt ttagtttctt cttctgtagt ttttgatgaa tcgaaatcat cgcaagcttt
    10201 atcatatcag cgctagttcc ttgaattgga ctattgatag cgactctctt tcctgcttct
    10261 tgttcttgtc ttttggtaga ttttatttct ggaatgtatc gtcttcttcc agtgattgtt
    10321 tctacataac ctttctcttc acaactaacg catatatctt ccatatattt ttttacacca
    10381 ggatattgat ggaagtattt atcgataaat attccagcgt cttgtcttga gacttttaaa
    10441 ttttgactga gaccataagc agttactccg taaataacag aaaagtttac tatcttagcc
    10501 ttgtttctca tctcaggtgt gacttctttt gccgaaactc cgaataatgc ctgggctgtt
    10561 tcattgtgaa tatctgcatt tttttcataa gcacgaatca tgtttttatc attcgcaaaa
    10621 tgagccataa tccttaattc aatttgagaa taatctagac tgagaagttg gaactcttta
    10681 ccatcagcaa tgaatccgcg acgaatcatc tttccttctt catctttgat tggaatattt
    10741 tgcaaattcg gatctgtaga agatagtctt cctgttgcag ctatcgtttg attgtagttt
    10801 gtgtgaatct ttttagttct aggattaatt aatgccggca gagtatctat ataggttgat
    10861 ttaagttttg tatactttcg atgctctagt aaatcttcaa taattggatg cattccaagt
    10921 agactttcta gaacactatg atctgttgaa taaccagact gtgttttctt ttcagctgga
    10981 agtttcaaat cttcaaacaa tactttctgt aactctttcg tagaagcaat gttaaagttt
    11041 ttatctgcat gaatgtggat tcgtttttct agcttttcaa tttccttctg aaactttgtt
    11101 gataaatctt tgaaatattt ttcatcaatc cgaacacctt gcatttccat gtctaataag
    11161 actggcatca ggggcatttc catttgataa aaagtatttt ccgactcact tatctttaat
    11221 tcttttttaa gtttattata caaacgaaac gttatatccg catcttctgc tgcatactct
    11281 gtaactcgat ccggatcaat gttaaaaagt tcttgtttct ttctacctgt tccgaccaat
    11341 tcatcataag tgatggtttt gtaatttaaa tagtcctctg ctaaatcatc cattccatgt
    11401 cttcggatac taggtcttat gcagtagctg gctaacattg tatcaaagct tacattttca
    11461 agatcaattc catgattttt taagacaatc atgtcatatt ttatattttg acctactttc
    11521 tctatctttg aattttctag tagcggctta atgtgattta tcactaaatc taaacccaag
    11581 catcccgaag caaataaaga ctgactgtat gcgataggaa tataataacc aacttcttcc
    11641 tctttactta aagcaatccc taataactct gcacgcatag gatctaccga agtcgtctca
    11701 gtatccacgc aaatcacttt tgccttttga aattctttta ctagctttac taactcggac
    11761 tctgttttaa ttcgagaata ctttccgtta gccgctttcg tattttcctt attaggttca
    11821 atttcactct tgactggtgt agtatctggt ttaattcctg cttgttttgc aagatctgta
    11881 tagagtcgat tatagcctct ttctttgagc ttgataattt tatctgaagc aagataatca
    11941 ggaaggataa gatcatctaa agtttctttt atagttaaat caatttttag agtagccagt
    12001 tctctagata gaaatgcatt gtctttattc ttctctaatt tttctttaag agatggattt
    12061 tttatttgag aaagattttt ataaattcct tctaacgttt tatactcagc aattaatttt
    12121 gcagcacctt tttctccaat tccatttaca cctggaatgt tatccgaact atccccaaca
    12181 attcccatat agtctgtgac ttgatcgggt gttataccaa tattttcaaa aacccatttt
    12241 tcatctatct ctgtaaactc ggtagctccc ttcgttcctc gatacatttt tatatttgga
    12301 tttaaagttt ggtataaatc tttatcaccc gaaaagatta agacttcatt tttcttattc
    12361 ccatactttt tgcataacgt cccaataata tcatccgcct cttgcccagc tatttgcata
    12421 acaggaaatc caagctctcg gctaatctcg attacctctc caacttgtgg ctttagatct
    12481 tcaggcatag gctttctctg tgctttataa gcatcaaata acttagaacg ctctagtgga
    12541 gttcctggat caaaaggaag agcaatatgt gttggcttaa agtcattgat aattttaaaa
    12601 agcattttaa aaaaaccaaa tactgcacca cttggttttc ctgtgagaga gtttgttaaa
    12661 tttgcagact gaaaggcaaa ataagcccgg aacacaaatg catgaccatc gataatgatt
    12721 aatttcatat tagttgccta taagtgatgt atatacttga attgttttct gaattgtatt
    12781 ttcaaccgag aaagctttta cactttcttt atttcgcttt ccatattctt tacggagagt
    12841 tttactttca attaatttct tataatgagc ggctaatcta gtgtaatcgc caaccggtgc
    12901 cagcaatgat ccattgccat cttccaacat ttctgaaatt ccaccaccag tcgttgcaat
    12961 aataggcaat tcatttgcca ttgcatccaa tacagaagtt cctaaaccct cttcttttga
    13021 tgttaaagta aatacatcaa aaaaggcata aaaatctttt atatcatttc taaatcctgt
    13081 aaaaactact ttgtccgaaa ttccaagttc cattactaaa tttttaagtt tagtttctaa
    13141 ttctcctgct cctactaaaa agaaaataaa ctttttctct gtttggataa gcgctattga
    13201 acgaagcatt gtttcatgat ctttatgatc aacaagagct gctacatttc caatgataac
    13261 cgtatcagaa ggaatattaa attcttttag cagtttattt cgattacccg cttctttaaa
    13321 tttatttaaa tcaatgccac tgtaaactgt aacaactttt tcgggacgaa ctccatcctc
    13381 aatcaatacc tctttgattc gattcgatac agttaaaaaa taatctattc gtttcgaata
    13441 atacttcctt agactcagag gatttttatt tatatgaaaa tccactcttc tagaaacaac
    13501 taatttggga gaaggaaaaa agaatttcgc aagtagagcc aaaccatgag tagctcctgt
    13561 atgtgtatga attaatttaa cttgattttc ctttgcaatt tttgctattt tatatgcagc
    13621 aatcaaatca tattcgccct tcatttttat aggaaaaaat ttaacttttt cagaaatccg
    13681 agactccaat tcagaatttg gttgtccaat taaaatttgc ggaatctttc tctctgctaa
    13741 accgcaaact aaataataaa gttgttgttc cccgcctctc catgttctcg aattatttaa
    13801 gtgcaatatc ataatagcct atcaaatatt ttatttattt ttattctgct ttattgaaat
    13861 gatgataaga attcacgttg tttgaaataa aactatatca gatgaaaaac ccggacccat
    13921 agccgagaaa agtccatact caccactctg aaacttttgt tcttttaaaa actcttcaat
    13981 tacaaataaa attgtaggag atgaaacatt cccaaattct tgtaaaacct tcatagaata
    14041 atagaattga tcctcgctta cgtccagagc ctgcgaaaat gcttctaaaa cttttgctcc
    14101 accaggatga aacagataat gctttatagc atcctttgct aatttgtttt gctccaaaaa
    14161 ttgattataa aaatctaaat aatttttggt aataagacct ggaatagatt tatcgaaaac
    14221 cacttgcaaa ccatccatct cgatttccca acccataaca tctaaagaat ttctccactt
    14281 tctagatgcg ttagtttcta tcttaataga ctgatccttt ttcttgccag agattatcat
    14341 agaaccagaa ccatccgaga ataatgaaag tgcaattagg tttgcttttc ttttatcagc
    14401 tggacgaaac gttagtgtgc aagtctctac ggttataagt aaaatatttt tatctggata
    14461 aagttttgat aattctccag cccgagcaat tccgtttgca ccacccgcgc aacccaaacc
    14521 aataattgga atccgtttta catccgtaga taagcctaat aaatcaataa gcggagcatc
    14581 gagtgtgggt gttacaaaac cggaacttgt gactaagata atgatatcaa tttctttagg
    14641 taaaagattt gcttgttcaa tagcaagttt tgctgaatta aatgcgagat ctcttgcatg
    14701 aataacaaat aattcatttt tctctttgaa tgttttagtt tctccatacc aagaaatatc
    14761 tttaaggaca ggtctagtct ttattttaga attttcaaat acaggtagat atttatctac
    14821 atctagattc gagtttctaa atacagcttt tgcaaactct ctaacatctt cttgtttcac
    14881 atttgtagaa ggagtggatt ttgcaactga gtgaatgtaa ctcataagaa ttacatagaa
    14941 aattagattt ataaaattgg aaattagttt ttatttttag attctgcgtt gaattctaat
    15001 tcaactatag agccatttat tttattttcg agaggattgt cggtaattgt ttcattgttg
    15061 tggtatagaa tctttacagt atccatcaga ctgcgaataa ttttcaaacc ttttcccata
    15121 ttcttgtgcg tcttattaac tccaccaaaa ggaagctgtc ctgcacttga ggattgatgc
    15181 tctttaatat catctagata atgatctaga ttcttaggag attcatagga atcattcact
    15241 gtttcactta tctttaggcc ttttccataa tcgagtataa aaagtgtaaa cttggaatca
    15301 acaatcctcc agcggcaaat gattgtttct tcactttgat tggaaacatt tgcagtaatt
    15361 gaattagtga gagcttcgtc agctgctaga atgatttgag atatatcatc ttcaccaaaa
    15421 gagttaagca tgagagtttt tctaagttct tttcgaaaat aacgaaccga ctccatgtca
    15481 ggaggaagaa acattacaaa ggaattatcg gcaacttgga tcaataagtc tttttctgac
    15541 ataatttatc ttttcactct caccatagcc gaattttcat ataaatttaa catagtctat
    15601 attataatca atataatttt tattttctaa gcctttttat ttagttccat ctcaatgtgt
    15661 tcaattcctg catctaaaaa aatttccccc tttgcgatga atccatgctt ctgataaaag
    15721 gtcagggcac tcaattgagc atacaaatag acgtttgatt ctaactcatt caaacattta
    15781 gtcaataaat tttttaccaa cgcacttcca atgcctaact ttctgtaatc tcttaacaca
    15841 gaaaatcttt ctaatttaat acctttatct gttttacgca tccttgcagt tcctacagga
    15901 ctatcttgat aatacgctaa aatatgaatc gattcatcct cgaactcatc atactcctca
    15961 gagagaagaa ctttctgctc ctctacaaaa actttcttac gtatagaaaa agcaatatcc
    16021 aattcttctt tggaaactat atcccgaact acaatcattt agaactagtt ccaactaatt
    16081 cagaaatttt tgagactcct tgacgaatca taaacctatc cagatactcc aatattgaat
    16141 aaggcaataa tggtccttta taaatatagc cagtataaat ttgaaaaaga tttgctcctg
    16201 cttgaatttt ctcaagagct gcttcaccag aatcaatacc tccaacacca atgatcggca
    16261 atcgaccttt taatttcttg taggcaaggc gtatcacttc tgtggaacgt ttctgaactg
    16321 gtagtccaga aattcctcct tcttcaactt ttgagtattt agataatgtc gttttatcga
    16381 ttgttgtatt tgtgaggata acaccggcta accggaaatc taatataaca tctagtagct
    16441 cttcaattga tttatcatct aaatctggag ctaatttgat aaatgtagga atcaaaaatt
    16501 caccaccaag tccttcttta attcccttaa ttaaagatat gaaagaatct ttctcttgga
    16561 aacttctaag acctggagta tttggggagc taatattgat taccccgtaa tctcctaaac
    16621 ttgaaagttt tttaaaactt ttcacatagt cttgaactgc attttctatt gcgactaact
    16681 ttgttttacc gacattaata ccacgtggta ttttttttgt ttgttttgaa attgtttcaa
    16741 atgctttttc tgagccggga ttattgaatc ccatacggtt cacaagggct gactgcttct
    16801 catatctaaa tattcttggt tttggatttc caggctgttc ttcaccagtg attgttccaa
    16861 tctcaacatg cccaaatcct aattttgcaa gaaaaggata aagctcacca gtcttatcga
    16921 aaccggctgc cataccaaga ggattattaa actgaattcc cgctatttca acctgaagtc
    16981 ttttgctttc gtatgtagtt aatttttcaa taatagggaa aacaaaaggc aacgaatttg
    17041 caagcatcag taaagacttg gacaattcat gagcattctc cggatttaat cgaaaaaata
    17101 attgttttag cgtagtttca taaataagtt ctctcgggtt aagcatgatt tgctccataa
    17161 cgatttaaag ctaatatttt ggatgcaaat cattgtatca atacattttc tagacgataa
    17221 acgctgagaa aactaagtga aagtgtctct agttcatagt tttttcttct agcatccttt
    17281 gtaattcctg cgcttttaaa ttacctggat ctaaatacat tacatcagaa agcaatttat
    17341 tcgctcgagc taaattgcca atctttcgat tcaagtctga caaattcaat agattattta
    17401 catttcttgg atctcttaat tttaaacatt caccataatc aattgcgaga gaaaaattcc
    17461 cttcgaattt ataagcatag gaaatcttaa atagataatc tgaatcaata ggattccaat
    17521 ttaaatactc ttcacacaga gggataaact ctttataatt tttttgagaa taaagtgtat
    17581 ctaatatttt ataatacacg tctttgtctt ttttaatttt caaagctttt tgatattgct
    17641 caattgcttg tggtattgat ttctgagcaa gaagtaaatc accattatcc acaagtgaat
    17701 aataatctaa atcaatctct tctaatggtt tatcttcttc gatgtaagat attttaatta
    17761 gagtataatc atcagtaaat tctccaacct taagaattga atctgtgatt cctttcaatt
    17821 cacctttacc ggtcattaca tgttttaaaa actgttctcc atcttcattt atatttcttt
    17881 gaccatcagg atccattcca agcattatat catctctacc gtccgatcca attaagatag
    17941 aatctcccgg atgcatctga aaaatttgaa ctcgtaagct tccatcaaga ccaatcatac
    18001 ctactttatg taaatacaat tgttgttcta aaaaacttgc ctttccatct cgatacaaca
    18061 cagtccaagg atgttctgca tttaggaaaa aaagtgctcc ggtattttct tctactaatc
    18121 ccatcacgat agatacaagc atactcccat caaacgaagt aaaaacattt tgcagttcaa
    18181 taaaacaaac tttcaaccat tgttctggaa ataattccat atttggttta aaaattttcg
    18241 ttctttcaat gatagatctg aaaacgactc ctagaactaa cgcaccacca gctccttgaa
    18301 tcgattttcc cattgcgtcc ccattgataa atacacaata attcttacct ctgagtttga
    18361 tattatttgc gatacacata tcgccgccaa tctcatatgt tttattttta aactcaaaag
    18421 tttttttctg tttaataaag aactcaattt taattttatt actacgcgcc tcatttacca
    18481 tgagaggttt tatcaggagg ctagttaaaa aatagtctcc atcttgctgc aatttcaatg
    18541 cttgcacttg tgtaagtgtt ttctgtaatt ctcttgttcg ctcttctact ttcttttcaa
    18601 gtgtaaggtt taactcttcc acttgactat gaactcttaa aaaccgattt gctaaaacaa
    18661 aagcaattcc aatcacaaat ataaagaaac catattgaag tagtccataa ttaggaatat
    18721 tcttacttat ccccatagca ccaataatat ccaatactcc tgtgctcact aatataataa
    18781 atccgaaaaa taatctcttt gaatcctgat gctttttaat aataccccat atcatcaggt
    18841 agatacaacc gataaacgaa atcaaagtaa agatttgcca aaccaataat aacttataag
    18901 ctattttaat ttcaacaaaa agcattggaa aagctaagat agatataaaa ccaaatgtaa
    18961 tttttgtagc taatcctgtc ttgcctttaa tgaaattttc aaaaaatgca agggagaatg
    19021 cagtaatgta aaataaaaat atatattcaa tgcgtgttat gatcattgga tcaatgccga
    19081 gttcataaac agcattgcta cgagtataaa tataaatagc taagaaagag cagaaggaag
    19141 caaaataaaa attatatttt tcatttgttc ttttaataaa aagtaacata tgataaaatc
    19201 caataaacga atagagaaat aatagcatca atgaaatacg ttcggagttt ttttgaatat
    19261 ttctttcatg caaatctatt gatggattcg aatctccata tacagcaatt tcctctcctt
    19321 gcactccctt aacgattata taaatttcat ttacgccgga atgaatcaat ggagtaggaa
    19381 tcttagttat taaatgacga tgaaaaccat gtcgcacaac gttatcttca gtaaaaatac
    19441 cctcttctgc aattttactt ccattaaaat aaatttcata ggcattagat aaataaggca
    19501 gccaaataga aacactttca gttaattgat tcatatcgct atcgctaata ttaacttctt
    19561 tgaaaaaact aaggacaata actttcttat cacttaaatt ttttacttcc gtagatttaa
    19621 ctggaagttc tttaaaactc tgccaggaag aagggttatt tttgaagttt aaatcaaaac
    19681 cttcttttac aaaccaatta tctttaatag aaattggaaa tgaataaata gaaattggaa
    19741 gaataaaaaa tgtaagaaat attttaaaca agaaacgcat cgtgtaagat tagaaatttt
    19801 tttttagcaa ttcaagcatt tttcaaaatg aacttagata attttattgc ttaaatattt
    19861 ctaccaagac ttcaccacta aattccttta ggtctacctc tcctaagtat acataactat
    19921 tctcaataga ccgaacagaa attaagtccg tctccccaag ctttttccct gtatctttag
    19981 ctctcacaac accttttaac tttaaaccat ctttgtcaga attgtagata attggatact
    20041 ttgaagactt ccagaatttt ggactctgct ctctattatg ctgcgcaagt cttacttgta
    20101 aattatgagc gatttcagtg ttaccaaatt tttcttttac ggaaagattt acattccctt
    20161 ttccatgaat cgaaataggt aagattttat caaaaatagc atttttatct attatgaatt
    20221 tcagtggatt attttgattt aacgctaaga ctgcattgct cccagcatcc aaaaaatacg
    20281 atagttcctt ggctgaattt gtgctattaa tgtattttaa gtgtagacgt ccgcttcctg
    20341 attcagaatc taataaatca aagtatattc ttggttgagt ctttttttta atctcaaatt
    20401 ctgtaataac ttcacgtttt tcaccagctt tcactggctt tacattccac cacaaaagca
    20461 ccgcaggatt attcgcaatg ttgatattct tatttgtaaa atacttccac tcggttggaa
    20521 aagctctatc ccaattcgca aaagctattt gatctggcgg agtattgtcg ttacccacaa
    20581 gataattacg aatcccatga aatgaatccg tttcctgttc atatgtttcc caataagtgc
    20641 ttttataggg tgtaaactta aattcattta aataaacttc tttttcttgt aagttatcaa
    20701 tgctaatctt tagaggaata tcatcatttg cctctgtgct aacgtctatt aagcttctaa
    20761 atccgatatc tataatacga ttagattgat tctttgcttc atatgaaata taaaaaaaac
    20821 cttctcgatt tactttcttt ttcaaaatca ttgcaatttc tacaggtata gaaggtattt
    20881 tacccaaaat tgtaatagat tcattttcca tattccattc agatcttaaa tcttctaatt
    20941 catgaaattt aaaaactttt tcatcaacct ttattgataa aaaagatgaa ccagataatg
    21001 cttttgtaac ttgctttaag cctttgtaat aagattctgt tttactaatc tctttatttg
    21061 ggttcaaggt aaaatttcca gatattgttg aataaactgg aatatttcca gtataaattg
    21121 caaaagatgg tagtgaagat tttctctctt ctactgaaaa caatggtgaa taaattccta
    21181 atccaaaagt taaaaaaaag aacgttagca gattgacttt attttttttt aatgaaactg
    21241 aattattcat tttttaacct acacttaaaa tactttatag ctgactgatt acctaaatta
    21301 tcaatagatc taaattcaag agatattttc ttctttttaa cagaaactag aggataatcc
    21361 ctgtataccc tccaattcaa agttcccttt tttctaaatt caattttatg aacaccagaa
    21421 cctaaatcaa aagcattgag ccaaatgagc gtctctctat cacagagtaa acctttacta
    21481 gaatgatttt ctggaattgt atctagttct acactcacaa tcggaggatc agaatctact
    21541 gtaaagttta aatgaatttc ttgcgataga tttccaactt tgtccattgc gataattctg
    21601 atttctttat gacccggact gtcaatcggt tgatctaatt tgctcaatgg caaataacct
    21661 ttgcggttta agtctataaa aatttcttct ataccggact ctttgtcctt tgtttgcaat
    21721 aatagtttga atccaggttt ataataataa aattgatttc tttcatagta aacaccttgt
    21781 aactgggcat acaattctgg aatcgttgta tctagatata tactttcttt ttttgtgctg
    21841 cgtagaacat cactctcatc gtatagatta tatactattg tatgaattcc ttgcttatat
    21901 atatttatgt ttggagtcag caattgttct ggctcatcat ctatctttat aaatactttt
    21961 cctttgaatt catttacaac ttgcggttta taatgtttat ttacataaat atcaccgtta
    22021 aaagaaacat aacgctttga cgtcttctct gattcggcaa tttgattttc agtttgtttt
    22081 cttagctctt tgcgttctct ttttgttttt aaaaattctt gtctctcttg ttcttttact
    22141 tcaaaccaag atgattcaga taaaacatag gcttcttttc gctcaattag ttcatctgaa
    22201 aaaatagaat ttaaacaaat gagaaataga aaaattattt taaatttatg aaataacatt
    22261 aacgtcctat cctcttttag atttgtgaat aggtagtgca atgcaactaa aaaaatgaaa
    22321 ttaaaataac atctctagat tattttgtag atagatcttt tacaaattct aaaatttctt
    22381 gtcgattctt ataaattgaa tttcgattaa aaaccaaacg agcagttgat tctaagatga
    22441 tttccttttc ttccaatcca tttgctttta gagtagcacc tgttgagact aaatccacta
    22501 tacaatccga cagaccacaa agcggggcta gttcaatact accataaagc ttaattattt
    22561 cacagtttaa acccttagaa aaaaagaatt ccttggttag atgtggatac tttgtagcaa
    22621 ctcgaatctt tctagaataa tttttcaaat caaaatctag actagcagca agagaaagac
    22681 gacatttacc aatttttaaa tcaactggag agagcaaatc ataacgtcct tcaatcaatg
    22741 tatcccaacc taaaattcct gcatcggctg cacattcttc aacataggtt gcaacatcct
    22801 gagcttttac tagtaagatt tttaagcgtg ccaatgaatc ggtaaacgtt aactcacgac
    22861 tcgattcatc cggtttttca gaaagccatt tctttttaat aagaagctct acactttcat
    22921 ctgcaagacg acccttgggc agagctatgg ttaacatgaa ttatcccttg ttggctttta
    22981 taagtaggta ggttgccaat tttttaactt cagaaatatc tgcagattca gattctatat
    23041 ccaatacctt ggtaagatct ttaattgcag catccttttc tccattcatc aacttcaatc
    23101 taccagattg atagtgagtc caagcagata aggaaatagt ctctctgttg tttgcgatga
    23161 gtgtagcagc tttagaaaag tcgctgagtg caagtttatt gtctttcgct ttttctctat
    23221 gtgaaccggc tacataatag taataagcct ttatttcttt tgaatcattt actagatcag
    23281 ctgccttttc taaataatct gcagccttac caaaatcaaa agactctaca tacaaatcag
    23341 aaattctttt atagattcgt aacttagcat cgtgggaaga ataagacttc aataaatctt
    23401 cgaagtcttt aattttctta gcactatcga tagaaaaatt ctttgcatgt gctttttcta
    23461 atttttcaat ttgaattgta gcttgatttg tgttgtattg gatccattcc ccaacggcaa
    23521 cgaatccaac aatcaagagt ataaaacctc cgattcccac aaaaaactgg aagcgattac
    23581 gagcaaagaa tcttgagaat ttgaagaaca ataattctgt tttagttcct tcaaaattct
    23641 caaggggatc atcgtaagaa actaacgatt ttttatactg ctcttcccta tatttctttc
    23701 tatcaaattt cgccatatcg attacgcttt atttgaatta ataaagctac caaagctttc
    23761 gctagaagga gtattatcag atttgatata tttagatatc tcttcttttt ccatagcttt
    23821 atcaaaatct ttaacggata gagatatttt cttattatca ggatcgattt taagaattac
    23881 tgctttgata acatcaccta ctttgtagat ttcttctagc ttctgtgtct tcgtatcagg
    23941 aatttgagaa acgtggatca atccttcata tccaggttca atttctaaaa agactccaaa
    24001 agagacaata ctcttgattt ttccttcaac gattacacct gtaggatact tcttgcgcag
    24061 aacttcgtaa ggattttctt gaagttgttt taaaccacaa gatatacggc ttgtatctag
    24121 attgatgtct aatatctgat atttgactac ttggcctttt ttcaaaagtg ttgtaggatc
    24181 ttttactttt tcatcccaag taatatcgcc tacatggatc aaaccttcga taccattttc
    24241 cacttctacg aatgcaccgt attttgttat tccagttact ttaccttcta atacatttcc
    24301 aactcttact tcagaggata gattatccca aggatttgga agaagttgct ttagaccaag
    24361 agaaagtcgt tttttagata aatcaatatc taaaatcata gaatctactt cttgtccttt
    24421 ctttaaaact tcttttggat gaggaggttt ttttgcccat gtcaattcgg agcaatgaat
    24481 caatccctct aaaccttctt tgagctcaac aaaggctcca aaatttgtta gagaagtgac
    24541 gattccgcga acaaccatat ctttttctaa ttctcgtcct gcccaaaccc atggatcttc
    24601 gtataattgt ttaataccta aactcaatcg attgtttgct ggatcaattt caagaactaa
    24661 tagttcaaca gactgaccaa cttggaagta ttgcttaaat ggagcatatt ttttataaga
    24721 aatatcattt tgacgtaaga gtccttcaat gccatgaaca ttgcagaata ctccaaagct
    24781 agcaacttta gtaacagttc ctgttacctt atctccaacc tttacaatct ttactaattc
    24841 atcccatttt tctttgttga tttcttcgat tagtttcttt ctagaaatga ctccagttct
    24901 gcctttctca ttcagtttaa tgattttgaa atcaagcggc ttcgctttta attcatctag
    24961 actggctttc ataccaagct gggaagctgg aaggaataat tgaacacctt ctacgtaaac
    25021 attataacca atgtttttga cttcagactc gatgcgaccc tgcacttgca agttcttgct
    25081 aaatgcttct ttgatggttt cccaaccctt tcgcgcatct gcttctaatt tcgatgcaat
    25141 gtagatttcc gagtcacttt ccttcgattt gataacaacg gataccatgc taccaatttc
    25201 aggtgtttct aaaaactcgc ttctagctac acgacagtct gactttaaac ctaaatcaac
    25261 atatacttct tgatcgtata catcgacgat tcttccttcg ataatttgcc cttttttgaa
    25321 agagcgttgt tctgtattgc ctctgtttgc tgatgaggta tttgttctat tgttgttcgt
    25381 ttgtgaattc aattttaacc aggaatctgt ataaatttga ccggcgaagt tacattccta
    25441 agctaggact gtaaaatctt ggacaggatc atattaataa caacattttt cgatagttga
    25501 gtagtgtcaa taaggattgc atcattcgct attttcaatg gagcgatttt tctttccata
    25561 tcagatttat ccctcttact tatgtcttcc tttaattgct ctaggtcaac ctggataccc
    25621 ttttcgatta gttctttata tcttctgtct gctcttgttt caatggaggc agttacaaaa
    25681 aatttatatt ttgcatctgg aaatacttct gtcccaatat ctcttccatc cataatcaat
    25741 ttatactttt tagcaagttt atgtaaagat tcattcacaa aatcacgcac atttcgtaaa
    25801 ggcgctaagt gcttgatttc ttctgtaatc tctggtaagc gaatttcctc tgaaatgtct
    25861 tctgaattca aaagagtctt gtttccgcca gagcttagaa actcagtttc aatattgata
    25921 ttcgaaaact ctttctctag attctgtgct tgaacccaat ctgcaaaaga tgaatttatc
    25981 ttgtttttct tatataaacg ataaaagaaa agggtaaaag ctctataata ggatcctgtg
    26041 tctagataat gatatcctat ttgtgcggca atttctcttg caacagtgct ttttcctgaa
    26101 cccgccggtc catcaattgc aattatattt tcattcataa aaatttactc ctgcaaattt
    26161 tcatctttac ctatacggtc actaatgcca ccgtcagtca ctatatagaa cagtgcggct
    26221 aatactgagt agcccactcc tatggaatga ggttgaatta tggctgttcc taaataaaca
    26281 tcaccaaaca aaccaatcaa tccagcagcc tgtattatgg actccgcatt ctttgattta
    26341 ccatccaaag cattaccaat tttaatacct gctagagtga tacagttgag atgaatcaat
    26401 agcaaaaaaa agattactaa tttcttaaac attccataca aagattttta ttgtaattaa
    26461 aaattccaac cttttacgaa caaagaaaat aaacatatca caaacaaatc ctaaaaaacc
    26521 ttattttaaa aaatctgatc tgatttcaat ctttagatct ctatctacat ttgaaaatta
    26581 atttgacaca agtagacatt tagagcaata aaagaatagg aaatggaaaa tctttttatt
    26641 ttactagaga atcccaagta tgcgggtaat attggaatga tttgtaggct tatcgcaaat
    26701 tttgacctac ctcctcttcg attgattggg gaagagaaag agttacattt cgaaatggaa
    26761 tggatggctt ataattcaaa ggaagaaatt gcaaaaattc aatattttca agataagaag
    26821 gaggcaagag cagatctaga tatattaatt ggaaccgcaa tgattcaagg gaaagacaga
    26881 cccggattta ttccgatcca atccctttat gaaaaatata aaagtttcgg agaaaaaaga
    26941 ataggtatag tatttggccg tgaggataga ggtttatcaa acacaatgat cgatttatgt
    27001 gatttgttga tcgactttga actttcaagc agacaaccat ctatgaatct ttctcaatcg
    27061 gtaagttttg ttttaggctg cttgcattct cccaaaccca aagaaaatat cgcacatacc
    27121 aaagatataa tagataaaac caatctttat aaatacgttc aacatgtttt tgagttaatt
    27181 gaaatgaatg attttcatgg aagggaaaat cttgcaataa aacgttttag caaaataatt
    27241 gattccagtt ccatttctag caatgatttg aactttttat ttaaaatttt taaacagatt
    27301 gagtataaac taaaaaagga aaattaaaat gatctatacc tatgtaaaat cttcaaattg
    27361 ttgcaatatc ttacaaattt ctatagcaaa gagcgaagaa aatctttaat cagtgaattc
    27421 tgggttcaaa acaattagat atgaaaagat tttgtgcgta acgttcttaa aatgaaactt
    27481 ttttaatatt acctccctaa actaaatgga tataaaacta aatctaacaa tgaataagct
    27541 aaaaaaaatt tcccaagtaa ttgtttatac aatagctttg tttttatttt ttagtatatg
    27601 tatagattta agttttgaaa ttaactacat ccgaatcaac tccgcaaatg actataaaaa
    27661 aataaatgaa gcgacagttg ctatagttct tggagcagca gtctatggca acaaaccttc
    27721 tcctgtctta aatgaccgtc taaaatcagc attaattctc tacaaaaaga gaaaggtaaa
    27781 aaaaattcta ctttctggag ataatggaac aaggtcttac aacgaactga aacccatgct
    27841 aaattacatg ctcaaaaaca aagtaaagaa agaagatatt ttcgtagata actctgggtt
    27901 ccgaactctt gacacattac ttagagcaaa gtatattttc caaataaagg acgcagtgat
    27961 tgtgacccag agattccatc aaccaagagc tgcttttatt gcagaaaaaa ttggtattca
    28021 agtttcctgc ttcgaatctg attccgggga atataaagat gaaaccaaaa acaggttgcg
    28081 agaatttttc gcacgtaatc tagcatggat cgatatgaat ttaatgtcgg atataaatta
    28141 ttcagggaaa agttatccta ttattggtga tgggacagaa acatggaagt taaaagacat
    28201 tccattagaa taagataaaa ctaaaatgga aacacttact ggttgttttg atttttttct
    28261 tgtaaataaa ttaatgcatt ataaaaatac tgtatcctat gaggtttcta gttctattct
    28321 ttctatttat ttcgcaaagt tttctctttg cagaagaagc tgtaagactg acaaacaaaa
    28381 aaggtgtcta caatgtaacc tcttatctag aaatgctaga ggacaaaaca agaaccttaa
    28441 ctcttagcac tattattaac tctgaaaaag aattatcctt taaacaaatt gatgatgata
    28501 cggttaatct tggttatact caatctgctt attggtttcg actaaaattg tttaataaaa
    28561 accgatcaaa accaactcta ctagaaatcc cttggcctca tattgacaac ttagacattt
    28621 acatgctgag caataaaaac aatggggatg aggaagcaga tgatgttttt gtttcataca
    28681 atagcgggag attaatccca tacaataaaa gaaactatga acatagaaat tttgttttta
    28741 aaattccaaa cttcccaaat ggagatgaac ttgtaatcta catgagagta gtttccgatg
    28801 aaacaattat ttttcctgtt tatctttatg atgaaatgaa attcttcaaa aaagaccaaa
    28861 gcgaggagtt tgtttttgga atttactatg gtgtagtcat catcatgttc ttctataatc
    28921 tttttatcta cctctcgctt cgagataaaa actatttcat ttatttattt tatgtgatcg
    28981 ctcttgggct ctatcaacta tcgatgaatg gaatcattta cgcatattgg cctaattatc
    29041 ctatgtggaa taaattgttc ctaccgcttt cctctgcaat acttcaattt attttaatta
    29101 ttttatttaa aaatctatta aacatcgaag aaagatattc ttgggaaaga aaatcccttt
    29161 ttgttttaac cattcttagt tttatttcaa tcattctcgt tattactgtt gactacgcaa
    29221 caattatagt tccgatgaac atccttggtt ttatctacat gattacttcc attttaattg
    29281 tagtccgtgc agttataaat ggcaatagaa cagcgatttt ctttttaatc acttggatca
    29341 cttttcttgc gggtggaatt atattgaccc ttagaaattt taatattctt ccacaaaact
    29401 ttctaacaac ttatagtttg caaataggaa gcactattga aatgctcctt ctctctctcg
    29461 cattgacaga tagaattaat actatgaaaa tggaattagc aattctcaat acaaaattag
    29521 aagaaaaagt cgaggaaaga acaaaagaat taagtcgcgt gttaggaatt ctgcaagcaa
    29581 aagagttaac gattgattcc gagctagatt tagcatctga tttacaaaaa tgtattttcc
    29641 cttcgaatga tttaccttat cctcatataa aattcgttgg atattatgaa tatctcatga
    29701 aagtcggcgg agatttttat gatgttgttc cacttccaaa taacgcagtt ggtattttaa
    29761 ttgctgatgt atctggtcat ggaattcctg ctgctctact ttctacaatg tataagatca
    29821 gttttatgaa tgcgactaga agaacaagtt ctcctgttaa aattttccaa gaagtaaata
    29881 agaatatatc cagtatcatg acgactcatg attatttaac tgcactgatt atagtaatag
    29941 aaccaaatgg aagaatttgc tttagctctg ctgcacatag acctgccttt cttttcagaa
    30001 agaaaacaaa aaaattagaa gtcgtttcca caaaaggaat gtttattgga atgcttagtt
    30061 atgctagtga tacatttgaa gaaaaagaaa gtaaaatgga tgtaggagat agaattcttc
    30121 tttttacgga cggagtaatt gatgcattta atgaaaaaga agagagatgg agttccgacg
    30181 aattacaaaa ggcatttatt aaatcgagcg gattatcact cgaagccgca ttggaatcga
    30241 taaaggctga ttggattaag ttcagaggaa atacaaaaat caacgatgat tcaacattgt
    30301 tactaatcga atacacaaat aagaaataaa actttctacc aaaaaatctt agttagttcc
    30361 atgaaatact ctttggtttc tgagacatca ttgtgtcctg catttgtaat ttgaaagaac
    30421 ttatagtctc ttttgatttt ggattctagt tttcgagcat aggcaagtgg gattacagca
    30481 tcttctgttc catgaaaaat ataaataggg cacttaacgc ttccaatcat actttcagag
    30541 tctaacttat attttaataa aaatgttggt atataaggat aatgataact tgcaatatct
    30601 gacaaattat tatacggagt ttctagaata aggcgtttgg gattcttatc ttttgctaaa
    30661 tacactgcca agccactgcc aagagagcga ccatacaaag taatttgatt ttctggataa
    30721 atagattttg catactcata tgcataaaga gcatctttta ttaagtcatc ctcagattta
    30781 atagttcctg tgcttttacc atatccacgg taatcgataa taatcaaatt ttgctttcga
    30841 tttaaaaaat catcagcaat tcctccccat ccagcaagat ttcctccatt tccatgaaaa
    30901 tacaatacaa ctccttttgc atctttcgtt ttaaaatgaa gtgcatttat tttaatacct
    30961 ggctctactt cccaattcaa ttcctcaaat tcaaaaggaa atgaataggt ataatttgct
    31021 gcgagaggat agggtctaaa taatagaaat tcttgttgag tgtatataat ggaagcaaga
    31081 agaacaataa aagccggtat aataaccatg ataaatttca ttggacaggt ttttatatat
    31141 tggttctttt gcttataaac tcacttgtaa atggaatttc aattctcgca aaagtaagtc
    31201 cattttttgt cccaattcta aacagagagg gatctagttt ttctgctttc aaaagaatta
    31261 aactgagaac aagtcccaaa ccttccccct cagtagaatc agaattatct aaataataac
    31321 tcattagatc atcatacttt tcacctaacg caagtttttc tctgatccta gattcttcag
    31381 ctggtagtag ttgtgtatta tttaaaactt ctatttcgag tccatcgtct gaataatcaa
    31441 atttaacacg aacaaatata tttctttctc gattgcgctc cccagattgt cgtaaatttg
    31501 acttattgat gtctttcttg aatagtaata tacccttcgc ataatcattt gggtcatcta
    31561 tattgaggga attttccaag aaaaaaagtt tctttgcatt tgctttaagt ccattcaaaa
    31621 tcgactcaaa taaaacatta tatacagtat tgactaacga tgatttgccg tatctatcta
    31681 atatggcggt aagtccgaat tttattatct cctcaatttc tgctgagaag gaatatacaa
    31741 taatcacaag acctttattt atattgactt gttctacgat attgactttt tctttttctt
    31801 cattgaattt ttcatcttca tagatgaagt aaaagtcttt ttcgttgaga ttttccattt
    31861 aatatattct ctcgtatact aatttaaaca agccttaact gcttcttctt ctgttgcaaa
    31921 aatttttgtt gtagtgtttt ttacagagac tttaataata ttatcaactg ctgttacaca
    31981 cgaacataaa tgaatgattc ccccaaattt atagaggcta acaagagacc taagaataat
    32041 accaactccg gaagaatcta taaaggggac ttccgccata ttaaaaacaa ctttatattt
    32101 tttttgctga atcagtttat taatagcctc actgattggc tcaatatcat acattgtgat
    32161 ttcaccctga atatcaatta ctggaatcga ttgattttcc ctgattgtta cattcatctt
    32221 aatgaccctt aaaattaaaa ctttgaaatt tattttgtta gtaagacttg aaataaattt
    32281 gccaaaatct tttagttaaa attattaaac tgatttaaag aaaagattct ttggtttggt
    32341 tgcaaatttt attatcttta ggtaatgaaa gcaaatttaa tagattcagt aaataaaaaa
    32401 aaagtcttgc gaataattaa tgtaaaagat agcaaaaaat aggataatac aaatatggaa
    32461 agaaataaag tttggtttat aactggttgc tccacaggtt ttggaaggga actatctaaa
    32521 ctcgttttag agttaggata caaagcaatt gtgacagcaa ggaatccaaa agatattcaa
    32581 gatattacgg agaactatcc tgatacatcg attcctatcc aacttgatgt tacaaataaa
    32641 ttagaaattg aaaactcaat cttaaaagca aaggctagat tcggaagaat cgatatacta
    32701 gtaaataatg ccggtatagg atattttggt gcgatcgaag aaagcgaaga agaggaagtt
    32761 agaagaatgt ttgaaatcaa tttttttggt cttgctaatg tcaccaatgc cgtcttaccg
    32821 attatgagag cgcaaagaaa tggaatgata attaatattg cctctatcgg tgggcttgta
    32881 agttttcccg ccgttggatt ttataatgca actaaatttg cagtcgatgg atattctgaa
    32941 gttcttgcta aagaaacatc gcatttagga atcaaggtaa tgatagtcgc accgagtgga
    33001 tttcgcacag attgggctgg aagatccgca aacaatacaa agaaaaaaat tttagattac
    33061 gaagaaagcg ctggcgcaaa tatgcaaaat attcgagcga gtagtggcaa tcaagctggt
    33121 gatcctgtgc gagcagtaaa agcaattgta aaagcagtag agtcagaaaa tccaccaatg
    33181 cgtttacttc taggaaaagc cgctctcaga aatgctcgaa acaaattaga ttcactcaag
    33241 aaagaattcg atgaatggga agaaacaacg gtttctgctg attatccgca atgaaacttt
    33301 taatttgctt ttaatagctc aaaaattata cgttaatacg attcttaaat tctaaagttc
    33361 ctttcagatt agaatttaag tagttaaaat gagctttttt gtaaaaggaa aaatcgattt
    33421 tcctgagtaa tttaaaatta cactcttatt taaatcctcc tattttttat tgtatttttt
    33481 ctatttatac ttgcataagt tttcatattc attattgttt gtccaatgga attagaggca
    33541 aaattagaga aacctgctat tgtaactgga acccgtcaaa tcaataatct attgattaaa
    33601 atgaaaactc ctcccgctgt ccacctacca cagagacaac ctcttgtaat aggacttgct
    33661 atcgacaaaa gttggtcgat gaaaggcgac aaaatggaag caacaattga agctgcttgc
    33721 tcacttgtta attggttaac tcgacacgat tatgttacta tcatagctta ttcagcagat
    33781 gtgcaagttg ttcaaccact cattcaacta aaagaaaaat cagccgtact tgataagatt
    33841 agatctatac aagtcgcaac ctccacaaat ttaagtggtg gttggcttca aacactaaga
    33901 gcggtcgaat ctgcaggtgt tccaaatgcc tataaaagag taatactact aacagatgga
    33961 caggcaactc tcggaatcaa agatccgtta caattcaatc aaatagcagc agaccatatt
    34021 ggtcgaggcg tatcaactac tactattggc tttggtgaag atttcaatga aacatccctt
    34081 cgcgacatag cagtcaatgg aggagggaat ttttattatg ttagcagtcc cgaacaaaca
    34141 tctgaaattt tctttcgaga atttggagat attggagcgt tatacgcaca agcagttgaa
    34201 gtaaaactaa aactagctcc aaatgtgaaa atgctcgaaa tatataatga ctttccattt
    34261 caatcaaatg aagatggaac aatatctatt caatcaggag atgttcgagc agatgacgca
    34321 cgcagtatcg ttatgtcatt ggagattgat gcagagaaaa atattgatgc tcccgattta
    34381 ttaaatattg atgtttctta ttacaatctc tttgaaaaga tgagattaga taagatcagt
    34441 cgatctctac cattagttaa aacagcaaag gaacctgatt ctgatagtga agttgttgta
    34501 gagagacttg taacatcatc cgctaaaact gtaattaaag ccgcaaaatt aatcaaagaa
    34561 aatgatgtag gtatggctag aacgttactc caagcggcaa tggatcgtgt agaagataat
    34621 attaaactat ctcccgatat cctaaaccca attctacaaa gattaaaaaa tattgaaatc
    34681 aagttaagag aaaacgctgc aagtgcaagt aaacatttta tggcagaggg aagtgatctt
    34741 tacagtagaa tggatatcat cgataccggt ggtgttgatg ttcatgaccg tatttttgaa
    34801 tacaaagtca taggggatat tgacttatac aaatgccctg atatcaaagc aacggttcaa
    34861 tcccaaatgc gcgatggtta tcgatttgta atttttgatt taagtgactg taagttcata
    34921 gattcatctg caatcggagc ttttattcaa atagtaggtt ggctacgaaa acgaggtgga
    34981 gaatttattg ttacaaatat tgtcgatagt gttcgcaaag tatttaccat cactcgtcta
    35041 gaaaatcata tccgagtagc aacctcaatg gatgaagcaa gagacattgt agaatctatc
    35101 atatctgcta catctagata aaaatatatc caaagtgcga tgattagttt tgcaagagta
    35161 gttttccaac tatcataaaa ttaactcatt gcacttccca aactccagca acaaaacaaa
    35221 gaaattcaaa ctcactattc ccaaacaata acagtggtta tttccaaatt gctttcgctt
    35281 taaattacta ttttttctat ctcaaatttt actaatgcca attaccaaaa gaattttccc
    35341 agttttttat agagagtctt atttttgata attgagaata cccaaatttt tcaaaaatgg
    35401 aaaattactt ttggcgcaga gtacacaatc atccaaaaca atttaatcga tcaatctcaa
    35461 gatttccatt acctatcatt ttctaggatg aacaacgaat cccttggtaa tttggttact
    35521 aaaaatttta ctagttttaa actttaatcc tttatgaagt ttcgagcatt cttgattctt
    35581 ttggctctaa ttttagctaa tttctgcatc tggcgcgtat tgagcaattc actagcctta
    35641 agtccaaaat ttggaagttg tgattcactt gttaaatttg cgttagataa attgtttttt
    35701 gcgaaggaac tttttaggtt ctcattcatg tctgaataat acataatccg cctccgtgcg
    35761 tatttgaatt attgtgtgtt ttatatcttt taaagagtag accctcatcc atgaagcgtc
    35821 tatttctcct aatataacta tcgattaaaa taaaaaatac ttgatactaa aaaaaataat
    35881 tgtacaattt ctttcctata ttattatgta caaaaaaaag aataattgtc agacacatgt
    35941 gtattaaact agtaaatcat acaggaaaaa gagaaggtaa gtttgtctct tatgaatttg
    36001 gaacagatct ctttggatac atctatttag ataaattcaa aggaaatgat gataaaggca
    36061 aattagtaga ccgatggatt ttaaacgatc taggaagtct agttcgtaca ttagatttag
    36121 aaatctacag aagagaagtt gaaaattacg aaaatgtaaa tgcaatctaa gattgccttt
    36181 catgatacat tcacttctag tcagtttatg aaaaattcag acgagcacaa aacacttcta
    36241 tcgcttctaa aaaaaagaat taacaaacta tctcaaaaaa tacaaactga gcaaaaaaaa
    36301 ttaagtaaac tcagtcttct aaggctcggc attttttcaa tcattggaat ttccattttc
    36361 atttattata caagcagaac agataatcat tattatctgc tcattatagg attagagatt
    36421 attacttttg tttttttgat tttttattat cgaagaattg aagtttataa gaacagacta
    36481 attgcttatg atagacttct aaagcgagaa acaaatagat ctcaatttaa actagaaacg
    36541 ttatatgact acgatgttac tatcaattct ctagaattga aaaaccattt ccacagagac
    36601 cttggaatat ccggaaaaaa tggccttttt acttatttaa atacaactaa aaccatcaat
    36661 ggagaaaaaa tatttttaga aaatcttctc cgcataaaaa gagaaagcac agatgaaata
    36721 gtcaaacgtc aatcagctat aaaaacactt tctcaaaata aaaatctttg ttttaaaata
    36781 ttgagaatat tcgaggaagc gcatttagaa gaagtaaaca gtaaaattaa tttagctcct
    36841 ctttattcct accaaagcaa gttcttcgag aagcaaaaaa tcttaagcag aatctatcaa
    36901 ccatatataa ttctaggatg gttagtaaat atatatcttt tcttagcagg atcgccatca
    36961 attagtgcaa gcctttttat aataaataca attctattta taattaatag aaaagaaatc
    37021 agtcacctat ttaagtcatt taaaaatatt gaagatgaaa ttccgtccat agaaagacta
    37081 acgttatatc tatatgggtc taaaattcag tttaatgata agaatacatt ttcgaattta
    37141 gacaaaaata gaatcaaaaa tctttataaa aaattgagtt caataatagg aagagtatct
    37201 gttcagaatg cacctcttat gcactacact ttgaatatgc tttttttatg ggatctatgg
    37261 cagttaactt cattttcaaa atggcaaaaa caaaatagag aaatcattca gtcaattaca
    37321 gatgatatct atcaaatcga ttctatactc cccttcgcat ttttgaagtt tcataatcca
    37381 ggctatacat ttcctacctt ggatgagaaa ctaaaaacaa tatcagctca aaatatggga
    37441 cacccattaa ttcctgaaag aaatagaatc aataatccgc tacaaaaaat caagattggt
    37501 gaaagtttaa taatcaccgg ctcgaatatg tctggaaaaa caacctatct cagaacagta
    37561 ggcatcaatg caattcttgc actctgcggt tcacctgttg ctgcagataa aatggatttg
    37621 cctccacttc atattttgtc tagtattaaa aatgaagatt ctttggaaga gggaatctcc
    37681 tttttttact ccgaagtaaa aaggatagca catatattaa atcaaacgaa agagccagga
    37741 atttatttaa ttctacttga tgaactatta aaaggaacga atacccgtga gcgtttaatt
    37801 gcgtctagag caattctaaa aaaacttgct tcatccaatt caattatctt tataactacc
    37861 catgatgtgg atttagctat aaaaaccaca aaacaaaaag tagctcactt taccgaaacc
    37921 attgaggaaa acaaaatgag tttcgattac aaaataaaag atggcgtgat caaatccaca
    37981 aatgcactaa aaattttaga aattgaaaac ctagatttag acttctaatc aatctttaaa
    38041 tataaaattt aaattctaaa atgatttttc attgactaaa tggaaagaga ttcagacaat
    38101 taagagagtc atgtttgatt aggagatact gccatcatag aattttttaa aactcccgat
    38161 ttaaagtctg ttcttataaa agcatttcaa aaaggaccag aaaatatttt atttcatgca
    38221 attcctagat acgtaggcga ttatctgcgt aattttacca tgcttgaaac agaaggcttg
    38281 gaacacgttc caaaggctgg acgtgttatg atagttccca atcatagtgg cattttggga
    38341 tgggatgcaa ctgtactaca aaacgaaatt ttacgagaaa agaaaagaat tcctagaacg
    38401 atggcacatg cattctggga gtcccaacct tttttaaaga aagttagccg caagatggga
    38461 cttatcacga ctgacttcaa gctagctgta aaaacattaa agaaaaataa attactagtc
    38521 attttcccgg aagcagaaca tggcaatttt aaaccttccc ataaaatgta tcagctaacc
    38581 gattttaatc ctggatttgt agccctttct ttaatgacag aaactccaat tgttcctgta
    38641 gcaattttgg gagctgaaga gaattatatt aatattggaa ctattgattg gtttgaagag
    38701 caaattggag caaagattcc tataccatta aatcttttac cacttccatc tagatggaaa
    38761 ataatttttt taaaaccaat cagcttgaaa aagtattctc gtaaagatgt aaaaaatgaa
    38821 aaattcttaa ttgaagttgc acaaaacgtt agattccgca ttcaagctgc aatacataaa
    38881 gaattagtaa accgtggtat tttcaaattt taagtagagg taaatatggt agatatatcc
    38941 actttaagta gaactgaatt attaaaaaaa ttaaaagtag aagatttaat gacaagagaa
    39001 gttttaacaa tagattatga tgatctaatt ggtaaagcag caagactaat gattgaaaac
    39061 catgtccaca gtctgcttgt tattaaatat gggaaaccta cctacatggt atccacctat
    39121 gatctaatac gagtatcata cgaagaggca tttaatgagg gtaatgcaga tatgcttcgc
    39181 actccagtgg aatctctcgt aaaaggacag aacctggtat ctgtcacaac tgatacatca
    39241 cttctagatg cgttagctct atttgttcaa tactcgatcc actccttacc ggttatcgat
    39301 gatgggaatg taatgggaat actaactctt atggatctag caaaatggta taagaagact
    39361 cacgagtaaa tttgaacaac gaagaatcct tttggtctga cgtatcagat tcagacatag
    39421 caagagaaag aaaaaaagcg aaagaattaa aacaaaccgc ttggtggaaa caaaaacgat
    39481 ccaatggaat ttgttattat tgtaaaaaaa agtttccggt agaagattta accatggatc
    39541 atttaattcc tttgatccgt ggaggtaaat ctattaaatc aaatctagtt cctgcctgca
    39601 aagagtgtaa caacaaaaaa aaacacaacc ttcctttcga attaggctag catctcgaaa
    39661 aagggatgat ctttaattaa atcatagtat tctttctgaa aagattcttt aactttgtct
    39721 gtagcattat aagcttgaaa aatgttttct tctagttttt tttcttcctc tcttagttca
    39781 taaccaacca tttgataggc aagcaaacca gctaaacgag taattcgatc tacatgtgaa
    39841 acttttccca tatcctctgc tgtaaaattt tgatgagcga ctgcatcctg taatccttta
    39901 ggcatattcc aaattttaaa aactttagaa cctacatcaa tatgatcgaa ggaaaatgct
    39961 tccttttcta aggcataaat gtctttagac tcgcttgtag cctgctttat catttgctca
    40021 taacgttctg taaatgtaag tgctaaaatg gtcatgccaa tttttttaag taaagcagat
    40081 aaaaatagat catctttaaa acttttcatt ccaagtggaa ttgatatatc atgtgcaatt
    40141 aacgcagaaa gaattggtag ttcctgtaaa ttcttttgaa agatatctcc ttttagtttc
    40201 ttagaaactt gtttgttgga ttgaagtaaa actaggtttt taacagtttt agttcctagc
    40261 aaagtaatcg catcctttag agtatggact ttaccggatc taccatagaa agaagaatta
    40321 gcaattttca taatatccat tgtaatagat ttatctggac taattatttt ttctaattcc
    40381 tcacttcctc cacttgggtt atcaatatca aaagttgtaa tttttacagc gactgccggt
    40441 aatggaggaa ttaccaaatt tgcaaaatta atattaactg ctggtatttg atctttggtg
    40501 actgacgtat cttcctttcg acctaacgct tgtgaaagtt tttgaactat ctgcatccga
    40561 ttataaggct ttttgataaa tgtatcaatt tttaattgaa ttaagctttg aataatattt
    40621 ttgtctgaag ctgcggatac tactgcaatt ttaatatttt tataactact acgaattttc
    40681 tgaataattt caattccatt tcctgcagcc atattcacat cgacacaaat catgtctggt
    40741 ttgtttaatg actcttgtaa gtagaaaagt aaatcctcgc ctgttgttga ctcatatatt
    40801 atatcaaatt tctcagattg tagatgcatt cgcagaagac ttctgtctat catagaatct
    40861 tctgcggtaa ttattttaaa tggttttaaa ttctctgggt ttatcccaac agggattttg
    40921 tcttcattca ttactacaaa ggaaccttaa cggaaagtaa tacgccttgc ctgttctcga
    40981 tcatttggat ctggtatctc atcaagggaa ttatatttca ctccatcaat tttgtaacag
    41041 atcacaccat tatcattttc aaaggtaatc gttttggagt tatttttcga ggattgaaca
    41101 gattcacctt ttaaaaattg tttaaacttg tcaaataaac ccatatgtat agacctctta
    41161 tttttttatt attgcgttac tagttacaac agaaattaaa cgatcgggat aatctgaaat
    41221 aattccatca accccatcat caattagttt taacatagtc tctttatcgt ttaccgtcca
    41281 ggggataact tttatttgcc cctgtctaca aatattaata aattttttat ccacatattt
    41341 ataaaatgga gagattatct caacttttaa ctctttagct ctatcaatga ttttctgtga
    41401 aggagcaaaa aaacctagcg ataaaagaaa aaattctaac ttggatggag caaataaagc
    41461 agatgtttga atttgtggat ttagtttttt tacttcaggc aatacatcta atacaaaaga
    41521 ttgaactgtt gcatacttta ctacatctgc ttcctctatc gtgtgcacaa tagttgttgc
    41581 gtattctaaa acatcttgct tggtataatt agattcatcc agctttgtct caatattgta
    41641 aaagaaaggt gttgttattt tgttatcagt ttcatatttt ttaacaaatt ggagaaattc
    41701 tgcgagtgtt gataattggg ttcctggaat tgttttttgt tccggaaaat ttttattttt
    41761 gagtgaacca caatctaact cttttaactc taaagctgtg aaatttccta ttggttcagg
    41821 aagaacaggc ttgccacttt tccatttaca taattcagga ttaataaaat tatcgtggtg
    41881 aataattaac ttcttatcct ttgttaagac tgtatccaac tcaatggtgg tcatctttaa
    41941 gtccatagca gctttaaagg caggaagtgt gttttcagga aataatcccc tagcccctcg
    42001 atgaccttgc aaatcaagat ttggaatggg tttattttca taaaatgaga atctacaatc
    42061 tgccaatgaa acaatcgcaa taaatagaat gattccgatt ttagaattcc tcataatagc
    42121 aacccgtcgc ataatcgata tttttcttat gttaaaataa cattttaatt cataactcac
    42181 ctaaacgttt aaataagatt tattatgtcg atcaaaaaag atactagaat cgaataacgt
    42241 aaatagacca agcgatagcc ttcggtgatt agaaattaca gtcgctttaa ctatatgtaa
    42301 tcgaatttat taaaacatta gcattctcta atattctcac aaacatcatc tattaaaaaa
    42361 ttctcaattt tacgtattgt aagttattat aactaatttg aatcagaaag gattttaagt
    42421 aaagaatgtt tttagatttg gcagaaattt attgacttga atacaaggtt agatataaat
    42481 tttatacata aatcttcgtt ttaaataaac aaatatcgca agtattccat agtttatttt
    42541 acccagattt tttaatgttt atcctactat taaaaataat tggaaaaaaa tttcagatat
    42601 tatagaataa ataatctgct tgtttctttt tctcaaattc ccaattgaat aatgtttctc
    42661 ttagttgaac actttttcct tctactgtag aaactagttt aaacttctca gttgctccaa
    42721 attcttgtgg aacttttttt aatacatact taccagattt tatttcttcc aatacatgaa
    42781 caaagaaagg atcatgttct acgcgagcaa gctcctgcat ggcaaaataa aatgttgtta
    42841 gccccttata ataccaatct acaattttat tttttccgac aggccacaat tgctctaaaa
    42901 aaaactcatt ttcatgaatg taagattttc ccttaaaagg tttctgtaaa attcgacttc
    42961 tcatgtataa ttcatgattt aagtctctat ctgagtctcc ctcataatca gtcccgatcc
    43021 atttaatcca agctttcttc tcttttgagg gaatcaatgg aagcaatgca aacaggtaat
    43081 tctgatcttt aaagattttt tcgtgactta aaaaatccaa tacttccatt ggaatcatat
    43141 aagcaccatc gttccattct aatatatatg gaatttcatc gaagtaggca tattcaggtg
    43201 gaatttccgt ttttaatgca tctccaaatt gggaaatcaa aaaaaccaaa gagattactt
    43261 catgcttagc taaactagaa aacacttgaa caaaattaca tttcgttcga agtgcagatt
    43321 gtatcatctg atactgttgt ttttcaaatt gatttgcaga aagaattaat ccaaagttct
    43381 tttgaaactt tacaagagaa ctttgtttga ttcgattgag atttccagat actagaaaag
    43441 gcatatgata ttagagtgct ctaaatccta tgtccgatct ataaaatgta ttttgttgag
    43501 gattgctttt aaggtattga taggctttat ctactgcatt tctaagattg tcagcataag
    43561 aactaacgcc taataccctt cctccagagg aaactaatat atcattttta taagtagttc
    43621 ctgcatgaaa aatttgaata tcttgtgaac ctgtttttaa attgagagga atatccttta
    43681 tgtaatcatt tggataaccg gtagccgcaa gcactacaac acaagaagat ccagagcgga
    43741 ttttaatttc ttttttacct aaacttccct tagcggcatt ataaaataac tctaacaaat
    43801 catcctcaat cataagcata agtgcttgtg tttctggatc accaaaccta cagttaaatt
    43861 caactacttt agcatattgg tcttgaatca taagaccagc atagagcaaa cctttatacg
    43921 gatgcccttt cttcttgagt ccttcaatca ttggtttgaa aacttctgtt ctgacttgat
    43981 cgtatactcg acttgtagcg ataggcgcag ggcaataagc tcccatacca cctgtatttg
    44041 gacctaaatc tccgtcataa gctcttttat gatcctgaag cgctggcagc ataataaagt
    44101 cttcaccatc acttaaagca aaaatggaag cttcttcgcc ttgcataaat tcttcaatta
    44161 caactctaga accacttgcg ccgaatctct tatccagaaa tatttctttc agggcattta
    44221 gtgcctcatc cttattttga caaacagtta cacccttacc cgcagctaat ccgtctgcct
    44281 taattacaac aggcaaacta tgagtatcca catattcagt agctaattta taatctgtaa
    44341 atgtttgata ctttgcggta gggatattaa actctctcat tagatttttt gcaaaatctt
    44401 tagacccttc caattgggcg caatacatag aaggaccaaa acataaaata ccaacttcct
    44461 ctaaccagtc agcgatacca tcaacaagtg gctcctccgg tccaacaatg actaaatcaa
    44521 atttttcctt tcggataaaa tctactgtgc tatttttatc ctttaagttt agagaatttt
    44581 taggtaatat ttcggattgc ggaaaaccac cattacccgg aaatactttc aattccttta
    44641 ataatttaga ttgtcttatc ttataagcta atgcagattc tctaccacca ctaccaatca
    44701 ataatacttt cataatcatc ctaacttttc tagactctta atgactgttt ctaattttga
    44761 ttttagccct tcgatttttt ctttttcctt tgcaattact tctggttttg cttttgaaat
    44821 aaattcagga ttcgtgagct tcctattgac cttttccatt tcagattcta aactagtttt
    44881 ctctttcgtc aaacgttctt gctctttctt aaaatcaatc attccagaaa gaggtaaaat
    44941 aatttcacct tttgaaaacg ctgcaacaga gtcagacttt tcaactgaat actctgcatt
    45001 ataggtaagc tcttctgctc tagagagctg cataattgcg agtctctctt cttctacaat
    45061 ttccttagct aataaatcag aagacttcac tatgactttg cactttttat caggtggaat
    45121 gttttgatcc gcacgaacca ctcttacttt cgagacaatt tcaattaaga tagaaagttt
    45181 ttctactttt tttgaatcga tatcagagaa aggcttcgac cagtcagagt ttgttaaatc
    45241 tttttcagca aatgctgaat gaatttcctc tgtaataaat ggcataaatg gatgaagtaa
    45301 attcaaagca gataataaaa catgaactaa cgtctgtctt gctacttcta atgatttttc
    45361 acctgactta ccataaattc taggtttaac caattcaata taccaatcac aaagatcacc
    45421 ccatataaaa tcatagatac tagcagccat ttcatagaaa tgatattcgg aatatgcttt
    45481 ttcataatca gctaaacatt gattgaagcg atttaatatc catgcatcac tcgcttccaa
    45541 gtcagaacca gaaattttct tgagagaaaa atcttcaggc agattcatta aaataaatcg
    45601 actcgaattc catattttat tacaaaatga acgatagccg tcaagtcgac tctcatcgaa
    45661 caatatatct tttccctctg gaagagttgc tgctaaaaag aaccgaaatg aatcagttcc
    45721 atattttgac atcatatcca aaggatcgat tgtatttcca agagacttgc taaacttctt
    45781 tccatgttta tcacgaacca gtccatgaat taaaactttc ttaaatggaa taacaccatc
    45841 gttaaatttt aatccaaaca taatcattct ggcaacccag aagaatataa tatcaaaacc
    45901 tgttacaaga attgatgttg gataaaaacg tttccaatct tccgtttttt caggccagcc
    45961 aaaaacagaa aatggccaaa gcccagaaga aaaccaagta tctaaaacat cttcatcttg
    46021 agaaatttct tcttttttaa gagaaggatt ttttgattta aaagcttggt aagcatcttc
    46081 taaactttct gccacaacca ttgaaccgtc aggcgaataa taagcaggaa ttctatgtcc
    46141 ccaccaaagc tgtctagaga tacaccagtc tttaatattt tccatccatt cgaaataagt
    46201 cttttcccac attttgggga taatctctat ttgcccattt cgaacaacat ccattgcctt
    46261 ttcagccaaa ggttttattt ttacaaacca ttgtgtagat aaataaggct caatgatagc
    46321 accacctcgt tgattgtgac caatagagtg agtatagtct tctattttct caactaggtt
    46381 taatttttgt aaatcttcta taatcttttt tctagcggca aatcgatcta aacctctata
    46441 gtctcctgca ttctcgttta gagttgcatc tggattcata atattaacgg gagtcaaacc
    46501 aagtctttta cccgcttcat agtcatttgc atcgtgagca ggtgtaattt taacaagtcc
    46561 actaccaaat tctttatcaa caaaggtatc aaataataaa ggaatttctc tattcattaa
    46621 aggtaaaata agagtaaccc cttttttatt tgcatatcgc ttatcgtctg gatgagcgca
    46681 aacagcaacg tcccctagca tggtttctgg acgagtcgtt gctacaacga tataatcaga
    46741 ggttcctttt ataggataac gtaagtgata aagatgtcct tttgtttctc taaattcaac
    46801 ttcaatatca gagatagctg tttttgtaac tggacaccag ttaataattc tctctcctct
    46861 ataaataaga ccttcctcat atagagattt aaataccttc gtaacagcag cagataaacc
    46921 tgtatcaaaa gtaaatctct ctttggacca atctacactt tcacctaaca tcttttgttg
    46981 atgtgtgatt tgtccgcctg acttagcttt ccattcccaa actttatcaa cgaattcttg
    47041 tcgtgtaaaa tcagttcttt ttttatttgc tgctgctaat tgtttttcaa caactacttg
    47101 tgtcgcaata ccggcatgat ccattcctgg aacccaaagg gctgcttttc cttttttacg
    47161 ttcaatacgt ataattatat cttggattgt atgatttaag gcatgaccta tatgaagcgt
    47221 tcctgtgacg ttaggtggcg gaattacaat agagaaattt tcttttctgc tataatctac
    47281 tttaaatgaa ttatttttct cccaatattc aatccatttt ttttctactg cttgtggttc
    47341 atatctatca ctaatttctt ttttcattca ttttactctt ttagaggtta ctgctcattt
    47401 ttattatgtg ttctcggtta aatcaagaag gacatgttta ttcttcatcg caaacatcat
    47461 gaaatcattt tccgttacag ccattctaac agcaaaaggc attagattat aaactgttct
    47521 aaaaataact ttcatttgtt catcattttt aatgacttca cgggcagcag gaccaacttt
    47581 ctcaacaaca tattttttta taattggtaa atttgcttcg agagtattaa ttacaaatcc
    47641 tgataattca tcacccatga ttttcctctg ttttctctta tttatggtct ttaattaaac
    47701 agaattttgc aaactcgttt agagacaaga attattaata gcaaatcaaa ggtcatccta
    47761 cccttttcgt cttttccgat gccaaagttc tagaagctcg aagcgtcaaa ctcattgttg
    47821 taatttgagg atttacagaa agacctgtag gaattacaga agcatccatg acgaaaatat
    47881 ttttatgtcc atacaattca aagtttggat caactgcgcc tttctctggg gagtcagctg
    47941 cttgaatcga tccgtgtgga tgagcagaag caatgttgat agatccagtc gtaattggct
    48001 cttttaagat ccaatcaaaa ttagattttc tatccacttt aaaaggagtt ttcattctag
    48061 taaaaggaaa aattaactct tttgcacctg cagctgtttg gacttcagct aacatcttta
    48121 aacccctcaa aagatttcta ccatctgttg gagtaatttc aaaataaact tttctttttc
    48181 ccattttcca ttctattcgg gcatttgttt ccccatctgc tccatctcga acaagcacaa
    48241 tgcctgcttg taaattttta tagttttgca tcacttcaaa ttgttgtttg ccgtaaaatg
    48301 gcataagaga agttgcgata gccggtcgat aaggagctgt ctccaaccaa aatccatatc
    48361 ccgaattatc ctgattgtgt ccatccttga ttacaattga ttgcggtgga ccagagaaca
    48421 tattaatctc ttcttcaaaa tgagcaaaga ttgtgctcgt tggatgaact ttaaaatttc
    48481 gaccaaccca attatttcct agaccagatc tttgcaaaag ggcaggtccc tcgatcgcgc
    48541 ccgcacttaa aattacaaca ggagctttta tctccatttc tcgaatcaaa tcatctggat
    48601 aggaatcata aggatctggt gtgaattctg cgattacagt tttaactttt ccatctttta
    48661 tttttactgc acgcatattt gtgattatcc gcgcaccgta ttccaaagca tctggaataa
    48721 acgttaggaa catagactgc tttgcattaa ttggacaacc aagaccgcat cttccaagtc
    48781 catgacatcc tcggttatta ttaggcaata cttccggatg aagtcctagt tgctctcctc
    48841 cgcgccgtaa tacattattg tttttattaa ttaatctttc aggaacttgg tgcactccta
    48901 gtctagcatg aatctcttct atatacggat tcattttctc cttggaataa tcttttaaac
    48961 caaatcgttt ttcccattca tctgttacat aaatcggggg ataaagagat gtttgccaat
    49021 tgacagtggt tgatccacct aacgttcttc cttggacaat attcaaagtt tgctcttctg
    49081 acagaacaaa tccagcatct ctgtagagtc ttgcattcga taaaaattca tctccattga
    49141 actgagcagg agtaaaataa gatccttctt caatcagaac tacattccaa ccatttttag
    49201 aaagttcagc agcagcgacc gatccacctg ctcctgatcc aattataatg acatctgcgg
    49261 ataattccca ttttccgctt ttaattttat tttctgatat aatatcttta tgttttttgg
    49321 gagtaatgat tttattattt atttctggta tacccatatt aaccttcgta tcctatcgct
    49381 ttttgatatt ctttattgct cgcaagtaaa aacatacagg tctgtctcag aatactaaaa
    49441 agacctcgct tcatttgaga atttgaattt ttccaagaca gtaatctttt ttctctttct
    49501 tctttgttta attttactag gggagtaaga gagaagtcca ataaaattgc agcgagaata
    49561 gaagaaggag cccctgccag ttcatgaatc aaatcctttg tgggaatggg agaagggtgt
    49621 ccataaatat agtcgtctaa cgcttttcct agatcaaaat taggaatagg atttccttct
    49681 agaaatattt cctgcattga cctcatgtta aaatattcct cagaggaaat tcctttgagt
    49741 gttggtgttg agagggaatt cccacaattt accccttcaa aggacaatgc agtaatcgca
    49801 aaagctttga ttgctaagcg taaaaactca cttctaggaa tatcttcttt taagaaattc
    49861 ataaactgct ttcctgttct ttatctttat ctttatcttt atctttatcc ttttcttttt
    49921 ctttatcctt ttcttttggt tttgttttag attttggctt tgtcttacta tcctttgttg
    49981 actttggttg ctcgcttgct tctttcggtt cttcgtttaa aattttattt acagtctcta
    50041 acttcatttc tccagtaaag gatactacaa atgcgcctgt ctcttttaaa atgtaactaa
    50101 ttggaaattc tattaaatac tgtgttccca agggtataat ttctaaatca atcgaacttt
    50161 cttgattctt ctttttaaaa actgcttttt taggtttaag ctctcctcca actaacaacc
    50221 tgtataccgg accactcatg attgcttctg ccatttgatt ctcttgcgtg cctgccggtg
    50281 gatttgtttt gttttgccgg aatataaaaa taaactgacc attttcatct ttaaacggaa
    50341 tcataggaaa agaatctgtt tctactctct tagtaagaga gctttctgat ccaatgtatt
    50401 ttaaatgtaa aattaaggaa gagtcagttt cttttttagc acctgagaaa gttccatcta
    50461 atttttttag ttcaggttta tttagtatat catctatatc taaatttgcc acaggagaat
    50521 tgactccacc cattgcaaac aaagctttag aaaaagagat tctatactct gctctaaatt
    50581 ttttatccgt gacttcaata tagtgaaata tatcaaaaca agagacacaa aaaaaacata
    50641 caaatagtaa aaacaatttc ttcataaaaa cctctgatca aaaaattcaa actgacacaa
    50701 cacaaaattg ataaatttag gaacaaaaga ggattttaaa aatattagaa aatcctaata
    50761 aactaataaa atcaatagcc tagggcatac ttagcagaaa ggcaacaaaa aagcgatggg
    50821 tgtaaagccc tctgttccta ctcatttgct ctgctcaagc tcattgccat tttgcaatga
    50881 caataggagc taacctcttt tgaacgcttg actcactcat attcaatcat tgctttcgtt
    50941 tagcattgat agtgtaaggt gagattctag tataaaatag aattgaagat ttttttccaa
    51001 ttaaaaattc taattctata aaagaaaact gtaaattatt ttacataaaa atttgataga
    51061 tgaaaagata tgaattcaaa caaaaaaatt ttcagatgca attggactgg ggatgactcc
    51121 cttatgatag aatatcatga tagagagtgg ggagtgcccg ttcatgatga taaaaaacat
    51181 tttgagtttc tgattctaga cgcattccaa gccggattaa gttggagaac cattctgcac
    51241 agaagagaag gttttcgaaa agcatttgca gattttgaag tagaaaaagt tgctcgcttc
    51301 agagaaaaaa aattgctcaa gcttatgcaa gatgaatcca ttattcgaaa tcgattaaaa
    51361 atatatggtt cagttcaaaa cgcaaaatca tttattcaaa tacaaaaaga atttggttct
    51421 tttgataaat acatctggca atttacaaat tataaaacga taataaatca ttgggagtct
    51481 atgaaagaaa ttccagccaa gacaaaagaa agtgatacaa tgagtaaaga cttaaagaaa
    51541 agaggattta catttgtagg ctcaacaatc tgttatgcat atatgcaagc ggcaggaatg
    51601 gtaaacgatc atttaaaaaa ttgctttcga cacaaacaag ttaaaaaata agcgtataac
    51661 caaagctaat aaaaaagatg tttcgaacaa tcttttgttg ttggtagttc tcagtgagtg
    51721 cccttgccac aatatcgtta ggcgaatttg cttttatttg atctgctaca aattgattca
    51781 aaggaccatt tactttagat ggatcaacag tctttccatc atttgcattg atatctccgt
    51841 aagttcctat ctttggaaaa ttacgatcag tatcccatag atagacgtca ggacgataga
    51901 catgattgag cgcaagaaag tattgaccga gaaaaaattg tatcttactt gagagatcag
    51961 aaaaaccact tcgtctatcc gtattattgt tattgtattg ataacctagt tgagagctta
    52021 tagaaaaacg gaaatattct ttttttctaa aatcgtgatt ccctccaaag ctcaaataag
    52081 tgcttccatt attcaaatta ttgaaagtgc tagtttcaga actaattcgg ttaaagatat
    52141 ttaaagtagg attcagtaac tctctaaaaa aggggagctt ccaagccata ttgatttcgg
    52201 taaatgtgtt ttttcgataa ggatctgtat tatttacata aaaaagaccg gttgtaaaac
    52261 ccccaaaaga gcccatttcc cagtcataat aaattccacc aattacaaaa tctttaaggc
    52321 cgttgcgttc agggtgtctt ttggtttgat ttggatcaaa tgtcaatttg cccgattgta
    52381 aatcattaag agatttattg gttacatcct ctccaccggg tccgttttga aaaagtctcc
    52441 catcagaatc aacgttattt ctatgagaga ctgcatttgc ggaaattaat tgcatgcgta
    52501 gacctttgag tggagccaaa acctgaaccg ctgttgcaag aacccaagcc tgtgcaaaag
    52561 aattataagg agtgttgtct cttctattca gtctttctcc aaatatactt gctcccctga
    52621 tgtttgcatc attgttcata gattgagaaa agaagatttt ataatcttta gctgttggga
    52681 cagaagaatt ctctttatta actaaatctt ctttagagtt tttaggatca agaggaggct
    52741 tttcttcttt caatgaatct ttatttgaaa gattcattga ttcgattttg tccttttgaa
    52801 ttttaataat aaacccatcg atagtcgtta caattaaatc ttcttctgtc tccttctcta
    52861 tggacttaac gtctatttta gttccacttt ttagcagtat agaatttgaa aacaaattca
    52921 aactaaaaaa tagaactaaa ctaaacaaga aaaggaattt catagtgtat gcacaatgtt
    52981 tctaaatagg cacttcaatc aataaaattt cagaatcttc caatgcggtg atagaaaaac
    53041 tttttgtgtc agaaattcca attccatctc gcttcgaaaa ttcctctcca tcaattttga
    53101 cacttccatt gataataaaa acatagacac cgttatctga aatatgattc gtatattcga
    53161 tagtctttcc attttctaaa gtagacaatg aaaaataaga atcctgatta atccatactg
    53221 catcttcact tggttctgga gaaacgacag tctgaatcga attgattcgc ttttctctat
    53281 caaagaattt ttgatcgtat cttggtttga tccctatttc ctttggtaga atccaaatct
    53341 gtaagaagtt tactaaatcc ttttctgaat tattaaactc tgagtgacga attcctgttc
    53401 ctgcagacat gatttgaact tctccaggat aaatcacttt ggcatttcct gtgctatctc
    53461 tatgtgctag ttcccctgta atcgggattg aaacaatctc catattttcg tgaccatggg
    53521 ttccaaaccc catagccggc tgcactatat catcatttaa aactcttaga gcaccaaatt
    53581 gcattctatt cggattataa taggaggaaa agctgaatgt atgattgcta tgcagccatc
    53641 caaaatcagc aactcctcta gtatttgcct tgtgtataat ttttttcatt ttgccctctc
    53701 tttaataatt cgacatcgaa ctattaaaca ttgttctaat tgattgcttt tttttcaaga
    53761 aaaatatcaa tttttatgct tcaaaatcag ggtattgggg aggaggattt ccttttttct
    53821 ctatcacctt aggggtaaac ttaacattac cagctggaac ttctgttcga atcaattcaa
    53881 tgataaaact ccactcatca ccataatcaa acaagaaaag catcttttta tctggaacgt
    53941 taaacacctc acctaccgtt actttagtaa catatcctgt agagcgaaaa ctagcatccc
    54001 catccattag gtaaatttct tgggattgaa tccaattttc gatattatca aaatagccat
    54061 actcatgatc caattcaaaa tcaaagcttg ttagtataaa ttgagcaagc atacttagag
    54121 tttgtttatt tggaattgaa agcactcgat aggatttttc tctgatacca ccacccaatt
    54181 tatcagaaag agtgactttt aaaattgaaa aagaaatttt tgattgtttc tcttgctcta
    54241 gtggattttt cttttttttg tttttcttaa attcatctag gttaattatt tcagccatat
    54301 taaatatctc ttatatttta ttgaatatct catttatttt cagtaaatca taaaataaaa
    54361 ctaattagac attattaaat taaatttaat agatcaactt atgcaaagct gcataaaaat
    54421 aacaactcca tccacttcca ttgtttgtaa caccgcaaca gtcgttctag tgcatcaatt
    54481 aagctattaa ccaaatccat ttatttacta ttccattaga aaaacgtaat atactaattt
    54541 tatttaaaat agttaataaa aaagaagcaa gtcagcttgt aatttctctt aaacacacta
    54601 aaactatgtg aataaaagaa tcctttctta taatcttctc gatgagtatc tctctaattc
    54661 ttctaaaaaa ctaagaaatt attctttacg cttgaaagct tttttttgta aaaaggattc
    54721 tatgatacgt attcacttgg ttctagtttt tattatttgt tttacagcac ttcccattct
    54781 cggagataat tttcaaaata taaatggatt ctttggagaa agagcaactg gtttagctgg
    54841 ttcctacact gccatatccg atgatgtttc tggaacttac tacaatcctg caggactagc
    54901 cttttcgcaa aactcctatt attcgatcaa tgcaagtagt tataacgaag ttaaggtaaa
    54961 ccataagaac ctttttggtc caggacaaaa ttacaatcga acatctagaa actatttacc
    55021 aaatttcata ggttttacaa gaaaggatgg aagattcaca tatggtttct ccgttttaac
    55081 agcagtgaat caatccttcg atcaatccga tcgcataacg cttccaattt atagaagaaa
    55141 tttatctcaa ttagacatta gttacacaga agaaaaattt agattgtatg ctggtccaag
    55201 tgtagcattc gcactgacgg aaaagttttc aataggtcta acaacttatt atatgtatga
    55261 tagcaatcga aagatagtga atcaaaacga aaaccaattc aacgaaaacg tgaccactgt
    55321 aacagaacaa aatagaagaa gaacgagtgg tttaatgcct gtgttaggat ttcaatacat
    55381 gctcacagaa aaattatctt taggtctttc tattagaaga atttttgtaa cggggggaaa
    55441 cgtttcctat agaactaacg ttactaccgc aggcaggaca ggcggtccat cttccattgt
    55501 aattcaatca acagaaaatg ggggagcaaa agtagatgga tttaatcctg ttttactcag
    55561 cccatcttcc tatcaagttc cacagacaac ggaagttcgc tttggttggg cttattttat
    55621 taatcgtaaa tttactcttg cgtctgatat aatctataca tccggttatg caatccaaat
    55681 tgccgataat agttataaca caagaacagg aacttttaca atttcagatc cgtatcaaaa
    55741 tgatttaaaa agaaatcata ctgtaaatta tagcattggt ctcgaatact ttatatttga
    55801 taatttcgta attagattag gtcactttac aaatcgctca aataatgata aagtgaattg
    55861 gacagaggct gcttggtatg ccgcatatcg ccagttaaac caagcgaata ccgatgtatc
    55921 aatcaggaat tatttcaatt attccatgcc agagaaaaga gaagagtata taaatttact
    55981 tggttatact ttgggttttg gagttgagac tgctagaaat tcttttagca tcagtataat
    56041 gattcaaggt ggcaagggaa ttggaacgat tgacagaaat caattaccat ctacatctac
    56101 atatagagaa agcaccgcct atctttctgg aagcacaaga tattagcggt taatagaaat
    56161 attccactaa aaaggaattt cctagtttat ttaaatagcc ttgtgttaaa agtctttccg
    56221 taatttcttt tatctcgtgt gaagactttc cttctaaaat gattcgaatg gattgatctt
    56281 tttcgttaaa gctttctatc tttaactgaa aaccttctat tacttgcttt gtagcattga
    56341 gccaatctga ataactgaga cttgtaactt cctctggata atagccttct atcatcatcc
    56401 catttgtctc atctttctct atcgtaatta gaagatcatc atcttcgctt ttactaatct
    56461 gtatatcgct taacgtgtct ttcggtaata gaattagctt atcaacaaat tcttctagtt
    56521 tagacatatc acctttaaga ataatttcct cttcctctat ttccatatca tcactttctg
    56581 aagaatctgt atcattcgat tcaaaaggag aaaagtcagt agaagaggca gttgagggaa
    56641 ttgtcttggg agttttcaat ctggtatctt ttcgaaaaat tttagctgtt agatacacta
    56701 aaccaccgag tgcaacaaaa agaattagaa ctgagtttag atcaaaggac tccataataa
    56761 aaccaaacaa aaagacatta aatcaaatcc ttagattgtc aatggttaaa ttaatttatg
    56821 caaggacaag cagctctata attagaataa ttaggattaa atccaactgt gctatagatt
    56881 cctctaaaag gagaaatcgt ttcatacaaa caaggaaaac ttcctgccca ctgaacatag
    56941 cctcctgctg gcacagatcc taataaggtg tttaaaacag tttggcaatt ggctgcactc
    57001 attgaaatcg aatccactcc taagttacca taagaactac aggtcgactg acaatcaaag
    57061 ccaacatttg ctaatcgata gcaaacacct ccaaattgga atccacctac acagtaggca
    57121 gatgctattg ttagattcgt aatggagtat ccaaatggat tagtagcagt gattgtatag
    57181 ttggtgctcc ctgaggaata gagatagaac tcgatatagc gcatgtcgtc gaagatattg
    57241 ttaaaccaag aggtaaatta ggattagctg aacaactttg cactgttcct tgataagtcg
    57301 gagtcaaatt aatattaacc cctgtggaca ataagaaaga attatcagag tattttagat
    57361 tagaaggagg aattggtaaa ttttgactcg tatcagaaaa gcgaccattg tttaaactcg
    57421 atgagaaatt aatattacaa taggtgccac catctctcaa aaaggcttta gttaggagtc
    57481 ccattttaaa atcgttacta gaaggatcac aaggttgaga atagtcttgt tagttacagc
    57541 tcaaaagacc caaataaaaa aaagaaacac ataaaaaaac aaatcttaaa attcttgtaa
    57601 ttcatagaac tcatcctata aaatgaaaat attttcatta gtcctcaatt ttagaatcag
    57661 aggtttggaa caaattattc tactgccttt ttcgatttta aataagaaac attttcctga
    57721 gcattgagca tagtttagga tctgctattc tactgcaaac cactttaggt gtagcacaat
    57781 cgatctgttc tttaaacaat acatttagat tttcctagtt cttttggcat gataggaaga
    57841 acaaagattt tctagttcat catttagtta cttgattcat taagaatcaa agcggtaatt
    57901 attgattttt ttaattcatt agcatattct aatattttca aaactatcat ttgttcttta
    57961 ctgtagcaat tttgtgaaat ctaattgatt gggaatatta aaatcccatc taaaaattgt
    58021 atcgttagac ataagttttt ctaattttat aaaatagagt cttgaatgtt tttttaagga
    58081 ttgtcaaaat tttaaactac ttatttcagt taattatgaa tattagaata ttgaccctta
    58141 taatcttttt atcatttggt gtaagtttat ctgcacaaga taagaaaaat ccatttccac
    58201 taccgacaag tctctcagac ttagaaggtc tttgggaagt taataaaatt gagcaaccct
    58261 acggatccac cgatacaagc gcagtgcagc aatggcttgg aaaaaaagtc tctgtttctc
    58321 aaagtaaaat ccaactactc gatgaatttg aactctacag taaatccaat tttgtaggaa
    58381 aagaatgtga tacaaaaggt ggagtagtaa gacctattac attccaaaaa tattccaaat
    58441 ttgatgcaaa caacaaagac tctcttacaa ttaaagcaga tgaaaaattg gcattaggtg
    58501 aattctattg caagacaact ccatttgccg aattcttata tgttccaaag caggaatcga
    58561 ttgctctcta cacgccaacc cctggagcag ctaacgcttc tttatcgagc ggtttctttg
    58621 tttatctaaa aagaatttct aaaacaccaa tcacaactct cagtgttcag tcaggagaaa
    58681 ttgcaccagc tagttttaat ttcaccgata aggctgttga cgcaataaaa gaattagaag
    58741 cttttagaga ctatctttac ttagatggca ataagaatta catctttgga tatggccacc
    58801 ttgtcgctcc caaccctcaa cttgtccgtg atggctttga acatccacaa gaagtaaaag
    58861 atgaaatcca aagatttaaa acaaaatatg gaactggatc aggagatgga acagcaacac
    58921 aacaagagaa aaatgaagct gtagatatgt atctccgaaa ggatttagat atcatcattg
    58981 ggaacatgaa aaaattcatt acagttccac ttactcaaaa caagattgat gcaatcgtaa
    59041 tttatctctt ctggagaggt tcttatgcaa atgatacaac gactaacggc aagatgattt
    59101 tggaattcta ccaattggta aatgcaaaaa acgatgcggc agttgctaac ttcatgagaa
    59161 ctcgactaaa gaaagatgta gatggaaaag atattccatt tagccctgga catccagatc
    59221 gccataacaa aacagctacc ctttacacag acggaattta cgcaaatcct tggctgtggt
    59281 aaaacgacca tctaggggta aatcctaatt tacccctact cggaaaccac tttttaaacc
    59341 actcactaaa tattcgcttc aaacaaacta ttaaatacta attatgaatc cacttcatct
    59401 aacccttatc ctatttttcc tctccttttc tctcttcgga caaacatttc cctcagacga
    59461 aaatcgtagc attaaaaacc ttacgcaagc gcatgtcgtt ccaactactg tcgacttgac
    59521 atatagtgag aaaaaaatta tcgctctagt taacggttct aaacaattag aatacaaact
    59581 cacaggtgag acaataaaca cagcaaaaga gtttgggaat atggttctag tcattaccaa
    59641 taaaaatttg atcgcggcat ccacaaatgc aaatgagttt gctagacaac ctttatatgg
    59701 tgataccaac attagtttta caatttcaaa ctgtatcgta tttgttgttt ctaataaaaa
    59761 aatctattct tacactgttt catcttcaga atggaggaca atagacttaa caggggaaat
    59821 tgttagaacg catgcggcat tcgataaaac aggagcactt attacaaata agagaattat
    59881 atcatattca gaatatataa aagaatttca tttttacaaa attgatgatt tcacaccgat
    59941 caatagcttt aacgtattag acaataaaat agtttttgaa agtaacgcta gtccgcgtaa
    60001 aacaattatc tacagggcac agacaggaga atattcagtt gtaaatttta attagtcgaa
    60061 ttctttggat attaagtctt tgactaaagg aagaaatgag attcaaaaaa acctttttca
    60121 tactttccat tttaataatt tctattagcg catacaccct gttatcccaa acaaactctc
    60181 cagctagtaa ttcaccagaa tcagcaaagc caatagaagc tggaacaaaa tatcctgaat
    60241 acatctggcc tatttttgga gataaactcg ttacaggtag ttttggagaa ttcagatatt
    60301 accattttca tatgggacaa gattttgcca cagaggctaa gaacggttta cccctacttg
    60361 ctatgacaga tgcaaaagta attaaactac aaagttttcg atattccatt ggaaatgcga
    60421 ttatcctatt acacgatgat ggctttatga gtcggtatgg tcacatgagt ggttttgcgg
    60481 aaaatatttg ggcagcaata caagataaag atgtattaga aaaaagaaaa cgaagacaag
    60541 actttgagta tacgttaacc gcctccgaac aagtaagagt aaaaaaaggt gatgtgatag
    60601 gatattcggg tgatactgga attggtccat cgcatttaca tttagaaata ttcaaagaca
    60661 atatttatta caaccctgct gactttgggc tcaattattt tgcctttgga ggtatcaatg
    60721 tctatgcggt agacattgtt cctgaaaata acaaatcttt tatcaatggt aagaacgcaa
    60781 cttatacact tcgtttcaaa cttgataaag ataataacct tgttccttct aaaaatgaaa
    60841 tcctaaaaat caaaggagat gtaagtctaa gtctctatgg gagtgaatct tccggtcgtt
    60901 ctaataaaat tggatttcaa aaaataactg tagggcttaa cggctccgaa ctacaagaag
    60961 ttaatttcac taaaatatct agctttcata ccttccgttc ttgtttcgtt ctagataatt
    61021 atagaagcag aatgaatggc agaccattta aatattttac acataccaaa gaggggaata
    61081 cacttcttgg atttaaaaat caagaagtag gggctggaat tgttcgttcg ggatctttta
    61141 aagatgggga aaatgaaatt agcctcggag tctatggaat ttccaaatta cctgcaatta
    61201 ttaaaataaa agcagtaaag gataacaatg agtatcctga tggagaaatt aaaaagatta
    61261 acctgagtca tgactcttat atcactgttt ctactcctga taaaaaagga gaagctttct
    61321 ttcctgcata ctctatcttt acacaagcaa atgtatcaat agagaaaagt aaaaaccaaa
    61381 aaatatccga aaaagatatg acacttgaat cagaagtcta tacagttgaa cctgactata
    61441 aagaatttaa tttaggatat gatctgtata taaaattaga taaagacatc aatccagaga
    61501 aatttggtct ttaccagctt tcgaataacg gtaaagttat aaagaatttt ccaggaattt
    61561 attacaatac aacagaaaga ttctttcgag ccagaattaa agtgacagga aattttgcga
    61621 ttttatctga ttatgcaaaa cctactctgc gtatctatcg atacaaggac aatcatgttt
    61681 tcaaaggaaa agattttaaa atctacatgg tcgcatcaga tcatggaaca ggaattccag
    61741 aatctaactt tcaagtcaaa gtagatgaaa aagaagcgta taccgattta gatcctgaga
    61801 ctggtcttag agaaattttc tatcccgact ccatgaaaga agttggcaaa catgtaatta
    61861 cagcaaccgc taaagatcga gcgaataacg ttgcagatcc tttggtgttt cattatgaag
    61921 tgaagtgagg tatttaagat ttaaccacgg ttaagcacgg atggacacgg atggggttta
    61981 atggttggtg gtgtttggat atttcattct atcgtttgtt tttattggga atgatttgta
    62041 agattgaacc acgaagagac acatgcggag gcgttggtcg cgaatgcttg gagagaaggg
    62101 aggatgcaca cggataatat ttgtgataat tatttaaaat tgtagaggta gatcacgatc
    62161 tgtctacgta taaaaaatca ttcaattcat cgttacgatt tcagaaaaaa attgttagtg
    62221 gaacctatta acaatctact aaacatcttt taaagttagg actaaacttc cctgaaagat
    62281 atcttcgaaa aaatatttag gatcagttta agggatattt atccctaagc ataagaaaat
    62341 tttggtgtta ttcgaattag aagtgatgac tagttgaagt tagccgataa tcattattgg
    62401 tattttgaat aaacatggaa acaatctttc accaactgcc agttcttcct acaaacccaa
    62461 ctgcatggga atatttttgg aaccagatgt cgcatggatt tccaacagga cttaccgttg
    62521 tgattggatt ttttattgcc atgcttggat tctggagcgc ttataacgac aaagaaaaaa
    62581 gggattacta catttctttt tctctactag ttttaggact taccggcttt gtgtttttgt
    62641 attttgtgcg gttcgtattt tgggacacag agagaattct atttttaaat ctttttattt
    62701 atccaatttt tagattagtc acttttggac ttacttactt tgcaatggtg agcacccaga
    62761 aaacaaaatt tatgaaattc ctcttattga ttactgtttt tatcgaagta aaaattttat
    62821 atcatacttt tttgggggaa ggatttactg gtgcatttta tgaaaatgaa ttgggaaagt
    62881 ttccaattat tacaaaagga cgtggcgttt tagttctgct tcccgctatc attggcttgt
    62941 atgatataat aagaatttcc tatttcatat ttttgaaaaa atctaaaatt catatatcta
    63001 ttgtcattgc agtgattggt ttttttattt tgaatataaa ttctgctttg ttagcaactg
    63061 gcgattctaa gctacctctt gtagtttttt cttttgtgcc aatgttttta atagcttatg
    63121 gagtttttca ttctgatttt ttaaatctaa atgaactctt atttaaaaag aatttattgt
    63181 tctatatatt ctcggtttca atttccattt tatttctatt tgtttctatt cttgtggctt
    63241 attatctaaa tccagaacat gggtttcatt ataggaatgg atatttttta atttcattat
    63301 tttcttttgt aacaacaata tgtctttctg catatatcgc tggtaacaaa ccagacgata
    63361 aatttgtaat gtttgttgca ttgaatattt tcatacaagg atttcttgca tttttagtaa
    63421 tgatacgagg attgtatcta cctctcatta tagaacatag attaaatcaa atcttgtttt
    63481 tattttttgc attctcatcg tcgattggat ttagaatttt atatttaggt tataacaaac
    63541 caataccaaa actctcctat ttgattgata tttccaattt gatttgttct ctatttgcat
    63601 tgagtcctta tttctttaat ggatattatg aatacaattg ggggagatat ccatctgcag
    63661 gatttttttt acaaatttcc caaactataa tcgttttagt tttactaaaa gttttatatg
    63721 attggattca atttagaaaa tctaataaaa attcacttgt tgattggatc atgatttgtt
    63781 caatcattgg agctttttta agcataggtt caattcctgc cacaagaggc atcgagttct
    63841 atccacttat caactttcaa ttcattgtaa cgtttctcta cgcttttgcg attataaaat
    63901 tcaatactct tccgattcaa ggagagtcaa cggctcttac aaatagaatt tctttttatg
    63961 ccatatttgt gattccaatt atactacttt tttattcgtc caatctctcc aaagaaatcc
    64021 aagggacgca aattgtttat catctttctt tgataggttc ctctgccctt gtttcttttt
    64081 ttgcatttac attcatatta ttacgaccga ttgctagaaa attagatgaa gagaaagata
    64141 aaattgaaga ggcaagaaaa caagctgata aagaaaaagc cattgcaatt cttgctaggt
    64201 tagaaacaga aaaacaaaaa aaagaaacag aagaactcaa caagcttgtt aaaagtttaa
    64261 atgaagagct tgatttgaaa gtgattatgg aaaaggtcca taagtatgta aaagaaaatt
    64321 ttggaattga atactatgga cttttcactg tatcacaaaa caatacttac ataagaccat
    64381 tggatatgta tttcgcagac tttgtaatgg aagaagatag atccaaaatt tttaatttta
    64441 aaattccaat taaagaagag agatcaggtc atgcttttcc aatcaaatca aagagacctt
    64501 tttatcttcc caaaatgaga acgttagccg ctacaaaaga ggaaatatac ttctctgaaa
    64561 aatgtaggat tcaatcattc attttaattc cactcttctt aaacaatgaa cctattggga
    64621 cattggatct ttataattct tctaaaagga tgaatctttc taaagatgat atcactcgtc
    64681 tttctatact tggagaacaa cttgctggaa ttatttatgg ctcaaatctt tataagcaag
    64741 tagaagaaaa aacagtagag ctaaatcgat ctttacaact gataaaaaaa gatctgaatt
    64801 tttctagtaa gatccaaaag aaattacttc ccagagaaag tttgcaaatt tctggtctaa
    64861 acatttcttc tttttatgct cctatggatg aggtaggcgg agattttttt gatataacag
    64921 tccatgaaaa taaaattcgg attttgattg cggatgcgac tggacacgga gtgcaagctg
    64981 ctttaattac aatgttgatt aaaagcgaat ttgaaacagt aaaacattct tcctctagtc
    65041 cgagtaattt attaaacgaa ttaaacagta gtttttgttc taagtattcc aatctggaaa
    65101 cgttgttttc ttgttttatc gcggatatcg atccaatcga aagaaaatta gaatatgcct
    65161 cagcaggtca tccagcacag ctactggctt ccaatcatga aataatagaa cttaggaata
    65221 caggaagaat tattggatta aaaaaagata caaactacca aaatataaaa atcgattttc
    65281 cctctggtag taaattgttt ttattcacag atggtttata tgaagaaata aacctaaaag
    65341 aagaagaatt tggagaggaa agattgaaac aaaatctatt ccaatacaaa gaaaatcatt
    65401 ctgatctaat catcgagaat gtaatctctg atttaaaaaa ttttcttgga gaatctgaaa
    65461 ttcatgatga tataacgttt gtaattgcag aatggtgaaa taatctacga ataccaataa
    65521 cttaacgttt gtatcattgg gaaagattta aaagattacc accgattgca ccgatggtgt
    65581 ttaagattta atcactgatg aacacggata atatacgtta tgatgattca atcacacttc
    65641 gaactcactc ggtataaact tcgagaacga aggggtgtat atataattgg gttttgttct
    65701 attacattcg ttttcgaatg catcacgctt aattttttag agattcaaat atttaccctt
    65761 taaatagagt gaatcgaaat tattaaatcg aaatatggat tcatcgtttc gatcgttctg
    65821 tgatcggtgt taatctaatt gtgggtaacg ctccctttct ctttccaacc tacgctccaa
    65881 ggtttcgcgt ccatcacttt cgctggaaaa tcagaggcat tcgaaataca atctacttct
    65941 tattagtatt gacaatcaac agattcgact taccgaagaa atgtaaatga atttggcgtt
    66001 agaacgatcg aatgctagga attggctgtt gcccaagatc atctgagaaa ggtctctata
    66061 gaacactcac ttctccttct tatttatttg ccattaatct gattacggca taatgctatt
    66121 atccgcacca actcagaata aagagaagtg agtatataaa tttatatatt atcttccgcc
    66181 aaagtagttc gcaatggcaa gaaataatcc tcccagaaac gcagccgtaa agttaggaac
    66241 actgattagc tctggaaaaa gtcctttgac tgcataaata ataaccgcat ttaaaaggag
    66301 tcccgctaca cctaatgtca aatagtaaac taacgcacca ataccaagag taaaaatcaa
    66361 aatcaactta cgtgcggcaa agtttaaaac aataaatact atgacgataa tcaacgcatc
    66421 tttaaaatca ccctttacac gaaagcctgc atcaatcatg ggaaataaaa acattacaac
    66481 taacacttgt aatacgatag aaactaaaag ttttttcatt caatcctcca atactcatac
    66541 tttagcattt ccctaaaaat acaacgaaat aaagaaaaat cttgattgac tgttttgtag
    66601 atcatagatt tgattctgta aattatggaa tgcattagga gcaaaaaatg accgagtcta
    66661 acatagcaaa ttcagagggt tcatcccgat tttcctatac agcgaaaggc ggagaatatt
    66721 ttggactagc gatcaaaaca ttctttttaa caatcttcac tcttggaatt tattggtttt
    66781 ggggaagagt aaaaatcata aaattctatt ataataatac caagcttcaa ggtgcttctt
    66841 ttgactacca cgcaacaggt aaagagaaat tcatcgggtt tttaaaagga gtaggaatcc
    66901 tgctatcctt cggtctaatt aattttctta tcagttatat cataacaaaa attgccaatg
    66961 caagtattgc aaatacgatt agtatttttg taacatatgt agttattttt actgcactgc
    67021 cttttgtaat cattctttct cttcgattca gactaagcag aacatccttt aatggaattc
    67081 gtttccagtt tttaggagat ccaaaagagt ttctaatctt atttctaaaa tcattggggc
    67141 taacaatcat taccctcgga ttctattatc catggttctt ggtaaaaatc acaacctata
    67201 tagctcaaaa ttctctttat ggaaatcatg cattcgatct tagactgaaa gcagaagaat
    67261 tatttattat ttttctaaaa ggattcttct ttacattgtt tacgtttgga atttatttct
    67321 tttggttcag agcaaatgtt tttaatttct tttggaataa ctattacttt caaaacggga
    67381 aaacaagttc caatcttact gggggcaaat tattcttaac cgcaataaaa tcaatcggac
    67441 ttatgattat aacatttggt tttggatttg cttgggtcat tatcatgaat caaagagtct
    67501 ttattgaaag catttctttt agtgctccaa ttgactttga tgcaatttcc aacatccctt
    67561 taaaaccagg ctctgccaca ttagatagcc tcggaggaga tgcgttagat tcatttggtt
    67621 ctatagtagg ataatgtttc gactccgcgt aatagagttt taccaccgat tcacactgat
    67681 cgcttcgata acaccgataa aagataggat aactcaaata tccatctttt ttttatcgtt
    67741 cagttatcgg tggtaatctt aatataaact tagaacgaac tttaaaacat ctgtgtgcat
    67801 ccgtgttaat ccgtggttaa aaaaattttc atcgaactca cgttgtttac ttttgtttta
    67861 atttttcgta agtctttgac ttcagagcta gttttatata atcttcttcg atttggtcga
    67921 ttggttttcc caaaagctct tctaattttt caattgcctt ttgcccaagt tcctttcctt
    67981 ggtaatttgg agacaaatca taaatataag acttcacaaa aggtatgatt ttattttccg
    68041 tccaaagata aacaaataaa aaccgagcag cagaataccc atagtcttca ttcatttctg
    68101 aattgagtaa gaattttttc agtggcttca tctttttatt tcgtataacg ccttgcaatt
    68161 caggtaatct ccaatttgta tagccttcaa taaacttatt gttctgaaca gaacccattt
    68221 cataaaagga tgcaaagcct tcattgaacc attgctgaac atcagtctcc gtattcaaat
    68281 ccacaaagga gtgaaccatt tcatgccaaa gagttccagg acccgaatta taataggtat
    68341 aaagcgttct ttctgtgctt gtaaaataat caggagattc aggaaaataa accccataac
    68401 tatcataacc cattttttta gagaactgct ctttgttttt aaagaaaatg attcgaagag
    68461 taaatctagg ctcatagata aagtatcttg catatgtatc atcaaaaaat acttttaact
    68521 tgtgctccac agtatccatt tccttttccg tgcactgata cgcaatatta taatatttcg
    68581 tatatcgcac tttgaagtca agtttgtaga ttgacttcaa atcttctttg aaagtggcta
    68641 tttccttttt ctttttttct tcatactcag ataaactgat ttgcttttct aaatagaaaa
    68701 tttttccatt ttcattggtg attttactca gcaaattttt tcgattgtgc aaagcatcct
    68761 ttcttttgga ctcagctcct atcgaatgaa ttaaaagaat aaaatagatt aaaaaaagga
    68821 aaaagcgaaa tgccataatt agccttttac tttctgaatt gcccgattgt ataaagcttg
    68881 gtattcacta cttccttttt tccaactcca atctaatttc attattactt gtcttttaaa
    68941 attcaattcc tctttgtcat gatagagtgt tagtgcacga tcaagagcat aactaagaga
    69001 atgttcaaca ccaggttcaa aaacaaatcc tgttgattta tttctatccc atgtctcatg
    69061 aacagaatct tttaatccgc caactcttga aacgataggc actgtgccat aagcatgact
    69121 atacatttga tttaatccac aaggctcaaa aagggagggc attaagaaga aatcagcagc
    69181 cgcttcaatc tttcgggcga gagcttcatc aaaaccttta taaaagaata ttctagcatt
    69241 tgaatgatgc gactcatgaa acaaagcacc ttcgatctgc ttatcaccac tgcctaaaac
    69301 aaaataataa aaaggcattc tccatctttc ataaaaagat tgtaaaaaag tatcgaatcc
    69361 tttttggtgc gttagtcttc ctattaatcc aacaagaggt cgatgtaaat ctacatgtaa
    69421 cttatattct tggtaaagag ctaatttatt tgctaacttg cctttttcaa ttgtttcaaa
    69481 agtatagttc ttataaatct tcttatcgag agcaggattc cattctttat cattgattcc
    69541 attgagtatt ccagttagtg aatcttgtct ttgatgcagt atccaagaca gagaatttcc
    69601 cataggctct tttaaaattt catctctata gccgtgacta acagttgtta tctcctgact
    69661 tacgactaac gcagccttca taaaattaga tttatgtaaa tgatcaaaga gatctagatt
    69721 taaataaaaa ggatcaattc gaagaaaccg gcacatatca actgggtgat ctccctgata
    69781 ggctagatta tgaattgtaa aacaagttgg ctttcgaaca ggttgtgtat tgcatagaat
    69841 ggttacaatg gcagtgtgcc aatcatgcga atgcacaaca tctacattta actctacagc
    69901 taaatggtaa caagcatagg aaaaaacagc aaaattaaaa tgttcgtttg cattcgcata
    69961 gatagtttgt agattttgga agataggcga atcaaaaaag tataattttt tatttccatc
    70021 tatcgcttct ctgaatttag acttttttaa gatcgcacat gcatccgagt gataggcaat
    70081 cgattcatca atacaaggaa attcccttcc tgtaaattct ggagcctgat ttagattttg
    70141 aataattggt aaagcaataa agacttcatt ttctttcgat tgataatcac tgagcgagga
    70201 gagcatatca gacaatcctc ctgctttaat ataaggaaaa aattctgcgg ttgtatgtag
    70261 tattttcaag ataattcctt tttttacttt cttttaaaag aaaatttttt cattttgaaa
    70321 taacgatatt aagagctgaa tctgatgaaa gaaaaaaatt taattttcag aaattctatt
    70381 tcattcagga ataaggcgaa tcaattaacc cccttttgga gctgtgatga gtaaaacatt
    70441 tattgtgacc ggtgcttccg gtggaatcgg aattcaaatt gtaaaaaaac tattggaaga
    70501 aggggcaatc gttgtcgcaa ccgatattga tttaaagtct ctagaggaat taaaaacaga
    70561 attttcaaat tacaaagaaa aattactcac tgaaatttta gatgttcgaa agcccgaact
    70621 ttgggaaaaa gttatctctc tcgcaaagtc acaatctgca cctttttatt gtcttattaa
    70681 ttctgctggc gtcttgatgc ctggttatat tatcaacact acacccaaag acatagactt
    70741 tcatatcgat atcaacgcaa aaggaataat gttaggatct caattagcag caaaatattt
    70801 tgtagaacga aaagcaggac atattataaa tatcgcatca cttgctggtc tttcgcctgt
    70861 tccgggcatc gcactctatt ctgcttccaa atttgccgtt agagggttta ctctagcact
    70921 tgctatggaa ctcgaaaaat acaatgtaaa tgtaacctgt atttgccctg atgccgtaac
    70981 aactcccatg cttgatttac aaaaagatta cgaggaagcc gcactcactt ttagcggatc
    71041 aaaaccgtta tctgccgaag aagttgccga tgtagtccta gacacgattg gaagtaaaaa
    71101 aatggaagta accattggtt attcaagagg atttcaagct aaactcgcag gaatgtttcc
    71161 agcacttgct tctaaaattg taaacgttat gcgattaaaa ggaattgaaa accaagaaaa
    71221 gttcaaaagg gatttggagt aattgaagta agaaaactta ataggtatat tgaaacgtta
    71281 aattacacca gcttcacttt gagtatctcg acaggcttga aataaattcc atctaggaat
    71341 gctaataaaa tcatcctgtt aagaaatttt attttttcaa aaaaaatata cttttcccta
    71401 agaaattttc actaaaaagc ttgcataaaa cagaataccc taggcatatg acatccatga
    71461 gtgaaagcga aaaataaaaa acgtgattat tatgtgaaat gtgccggcta acacctaaca
    71521 cctaacacct aacacctaac acctaacacc taacacctaa ca
//
LOCUS       DAOQOS010000011        70632 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_126_length_70632_cov_6.834932, whole genome shotgun sequence.
ACCESSION   DAOQOS010000011 DAOQOS010000000
VERSION     DAOQOS010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 70632)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 70632)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..70632
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126_length_70632_cov_6.834932"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3029
                     /locus_tag="PLF66_03885"
     CDS             <1..3029
                     /locus_tag="PLF66_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002154852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HMZ63211.1"
                     /translation="MINDLLEKSDFSTIAEKLNYPLSKEILLARSISYLDDSLEVPSH
                     LLDQINLNQNKNIPVIGLTGTGGAGKSSITDELIRRFISDFPDKKIGIISIDPSKKRT
                     GGALLGDRIRMNSIYHPNVFMRSLATREANLSVNKHISQIISLYRSFYFDIVFIETAG
                     IGQSDSMITDVADISIYAMTPEFGAATQLEKIDMLDYADLVVVNKFDRFGAEDALRSV
                     AKQYQRNQKLFDQNLEDMPVFGTIASKFNDEGMNTFYLSLLSIIHKKTNILFESKLYK
                     DSRNKNQRNRSGIIPQSRTRYLSEIVASIEEYNQFAITQSELARKMFCLKSSIELLLP
                     EKQDWTGLDLHFHEKVPFDENHFASFGISELLKLYKSYLNELHPECKVILESWEKKVK
                     NYKSEYFIYHVREKEIKVANYSKSLSDLDIPKITLPRYKDWGELLFWSLQENVPGEFP
                     FTAGVYPFKRAEEEPTRMFAGEGGPERTNRRFHYITKGQNTIRLSTAFDSVTLYGENP
                     DERMDIYGKIGNSGVSIASLDDIKKLYSGFNLCEPSTSVSMTINGPAPMILAFFLNAV
                     IDQQCELYIKQNGLLKEVESKIQQIFKEANVNKPHYSGELPDNHNQLGLLLLGVTGDR
                     VLPKEIYDKIKMDAISKVRGTVQADILKEDQAQNTCIFSTDFALKLMGDIQEYFIQNK
                     VRNFYSVSISGYHIAEAGANPITQLALTLSNGFTYIEYYLSRGMKVDDFAPNLSFFFS
                     NGMDPEYTVLGRVARRVWAKAIRFKYKGSIRSQLLKYHIQTSGRSLHAQEIEFNDIRT
                     TLQALTALYDNCNSLHTNAFDEAITTPTEDSVRRAMAIQLIINREMGLAKCENPTQGS
                     FIVEELTNLVEERVLQEFIQISERGGVLGAMETMYQRNKIQDESLFYENLKHSGKLPI
                     IGVNTFLNRKGSPTIIPDEVIRSTQFEKDIQISNVRQVETANSHFAVHGMDQLKKAAR
                     ENQNLFSYLMEVSKFLSLGQMTNTLYEIGGQYRRNM"
     gene            3030..3890
                     /locus_tag="PLF66_03890"
     CDS             3030..3890
                     /locus_tag="PLF66_03890"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HMZ63212.1"
                     /translation="MSLYEHSLTTRHCEMDSSMHINNANFLRYTEEARVHMMVAQQFP
                     MEEVHKANVEMILYKSVSLYKEQVRYPESLTVKSKQIQTKKVRGILRQEIYKEDGSLC
                     FVSDAYWAYHAKDKSAVDATIQFTKKFGADSDNTILPLEYDNNFKASHNYPHVNVKIE
                     VRPYELDSFQHVNNAVYANYFEIGRWEFRRTVLPDLNYFKDMGLTFVIYKNTIEFIRP
                     AFLFQELNIRTWLIEITPARIIYWQEIEGENGTIHANCRSEGCVIDKAGNPVKLKPEI
                     IRAYNALLVH"
     gene            3996..4523
                     /locus_tag="PLF66_03895"
     CDS             3996..4523
                     /locus_tag="PLF66_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008589274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63213.1"
                     /translation="MKLSIAKISSERAKLLSAKTPEQYIALSINSKLPPGKKATITSE
                     WLAKTNYTYEDISYARNRDPYWRKKKSLGSKQRNLKRISEFNYKKPGSTPKKTWSVKE
                     MEELFELDKKLVDRELAKKFKTSLPAINHIRRKFKMVREIFEIRKQKPNKSKIVAYSM
                     KSEKLLRNELNSLSK"
     gene            complement(4526..4936)
                     /locus_tag="PLF66_03900"
     CDS             complement(4526..4936)
                     /locus_tag="PLF66_03900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63214.1"
                     /translation="MKNFFLLLAIALILSNTNCKNKKEAELNDPEWRKESMEIAQEIC
                     KKLSSCISKEYSILKKGLQTYAMSEIAPEKCADKNKKSRVYLLKGNDPQFIKETTRDC
                     YYQIQKFSCEEIVSGSIRKSKSCQMMKSIQQESL"
     gene            complement(4949..5686)
                     /locus_tag="PLF66_03905"
     CDS             complement(4949..5686)
                     /locus_tag="PLF66_03905"
                     /inference="COORDINATES: protein motif:HMM:NF025057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HMZ63215.1"
                     /translation="MLPNEMNTKSKKRRYPKHKEEKDMSERWLLTYADMITLLLGLFI
                     VMYSISTVDLGKLKSVSSIIRGGFGLDDGGDTLVLDGSDGIIKDQDLVPKSPLYRLWE
                     KFQSSVKRLLISEKIIIDLQSNEELTLTIPASSLGEGNIKLTKDSDELFSKLAEVTKD
                     LNIEIILRVQIPYLDTIENKKDLNNWAYNSYRASVMAKFLSEKYGIPESRIAVQGLSG
                     FRKNSAAETPEEAANQERIEIMIRKRE"
     gene            complement(5689..6336)
                     /locus_tag="PLF66_03910"
     CDS             complement(5689..6336)
                     /locus_tag="PLF66_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008595691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63216.1"
                     /translation="MIHYSKEEIFAAKKQVVKAQVDRFKVTYKNYFERPDTIQMVNYF
                     FDKIYNLDAKAEWVDLAISSFDKIKSMIKDETRESMERLIELNNLTDKLDTQMAYLII
                     EKNWTEGYDLTPDEFKKYFIELGHSEERFKQLEIVLLNLRQFYDLAHRPINAVLIKPA
                     RFMAKILGIYPLFATIEEGYHACLPVNRELFESFFAEVDKTEHEYLRSCFPNFGK"
     gene            complement(6628..7614)
                     /locus_tag="PLF66_03915"
     CDS             complement(6628..7614)
                     /locus_tag="PLF66_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010572956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA photolyase"
                     /protein_id="HMZ63217.1"
                     /translation="MRKFSHIYIESDAYNYPITKDILEKFPDSIQLEIKDYKHIFNRP
                     NQDFLSQKNSIKLILAVKKDNFYYPGSNVVNNYGYKDFYYNTLVLNCLYNCEYCYLQG
                     MFNSGNIVVFVNLEDFFDATRELSHNHPIYLCISYETDLLAFERVVPYTSKWILFARE
                     NPNVLIETRTKSNQYNLIQNLTPVKNVILAWTISPEIVIKNYEKGAAVLNQRLNNIKN
                     ATKDGWNVRICIDPILHLENWKEIYKSFIQKTFSELKESKIYDISLGSFRINADYLKK
                     MKKMRTNSKLLYYPFERQQNLSAYKKEKAQEMISFVASEIETFINKERIFIS"
     gene            complement(7611..8663)
                     /locus_tag="PLF66_03920"
     CDS             complement(7611..8663)
                     /locus_tag="PLF66_03920"
                     /inference="COORDINATES: protein motif:HMM:NF014503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63218.1"
                     /translation="MGLFKFSIFVFLIASFLPSCSNSSKDKNKKLIQQIIAFYFSRTT
                     DNGENAAKIFYINPLVKSGSSFNLDLVAEATSANVTSGKKKIVLVHGWDYTDRDSTSL
                     SLTQQKTRIINDSWADFIKTTTFDQIVATKQYDIYGFDYLTSNSIDINGKRFRARLDA
                     LFAKETGTVVILAHSMGGLITRFALYEATRPAYIARIITTGTPYHGSPWASSQFQSDK
                     GTLGNIASFLTNTSGGKDLAWDNFDSKISGASNSKLTTINQKTDRDDLIYAYYGSVLS
                     SQSSGGSGASTPGLYLSCPVLGSSFSPSDCIVPVSSASLSGNTLKQIRDFGQYDHFDI
                     KFTTSTMQSTLYSDLP"
     gene            complement(8668..9003)
                     /locus_tag="PLF66_03925"
     CDS             complement(8668..9003)
                     /locus_tag="PLF66_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000406877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HMZ63219.1"
                     /translation="MEMIERESNSIQIIDIIGEVDLYNTKEIKDMIDEKIKQGKYQII
                     LNLLKVPFMDSSGIGTLVTGMYRLKKYQGSLKIINIVGSVAKVFKMTGMDTHLEIFDS
                     EEEAIKSFK"
     gene            complement(9304..9648)
                     /locus_tag="PLF66_03930"
     CDS             complement(9304..9648)
                     /locus_tag="PLF66_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016546707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HMZ63220.1"
                     /translation="MKLIVAIIQPHKLEEVKAELTKNEIYRLTVSDVQGYGQQKGKTE
                     VFRGHEYQVNLLRKVRLEIAVNDEFVKPTVDAILKSAKTGEGKIGDGKIFIMPIEEVI
                     RIRNGERGSSAI"
     gene            complement(9660..10976)
                     /locus_tag="PLF66_03935"
     CDS             complement(9660..10976)
                     /locus_tag="PLF66_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010415306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HMZ63221.1"
                     /translation="MLKVNKLKYLVLFLVMVVIPTLLNGQDATTPAPAPAPAPAAPTL
                     DKADTVWLIVSSALVFFMIPGLALFYGGIVKSKNVLSTMMHSFIAIIVLTLQWTIFGY
                     SFAFSGTNPFYGDFSLAFLNGIDVDTLEGSIPKFVHFLFQGMFALITPALISGAIAER
                     IKLSGYVVFILLWATLVYDPVAHWVWAADGWLFKDGALDFAGGTVVHLISGIAGLAAA
                     LVIGKRKGDPSTLTHPNNMTYTLLGSGLLWFGWFGFNAGSGLKIDGLASRAFVVTLVA
                     PAAAGAMWLLIEWIHTKKATALGAASGIVAGLVVITPAAGFVSPVSAIIMGFIVSPIC
                     YGAILLKGKLGYDDTLDAFGIHGVGGAFGAVLTGVFAMSLADGVTRGHQIQVQVVSVV
                     ATGVYSFIISYILAFLIEKSIGFRIDEDKEINGLDQEIHGENGYGI"
     gene            complement(11262..11627)
                     /locus_tag="PLF66_03940"
     CDS             complement(11262..11627)
                     /locus_tag="PLF66_03940"
                     /inference="COORDINATES: protein motif:HMM:NF036460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5329 family protein"
                     /protein_id="HMZ63222.1"
                     /translation="MLKKMYSILFLFLLIPQLSFSQTKHISTESDKIKFLLNELDKPN
                     SNLKFIRNGEEFSGREAKEHMEKKLKVAGERIKTVDDFINQIATKSYLTGNIYYVKLP
                     DGTKVESAKWLRETLKKLN"
     gene            complement(11631..12731)
                     /locus_tag="PLF66_03945"
     CDS             complement(11631..12731)
                     /locus_tag="PLF66_03945"
                     /inference="COORDINATES: protein motif:HMM:TIGR03806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SO2930 family diheme c-type cytochrome"
                     /protein_id="HMZ63223.1"
                     /translation="MAKLRIKMNLNSFLLNLSLFLFLVLFFYCKPSNSVHAYLEEPYP
                     EKLSDWKLFIGNISELKPNDGVLPYDLNMPLFTDYASKSRFIWMPKGKSAQYKEEGTF
                     VFPTGTVISKTFYFEGAKIEDSNFPKQLVETRLLVNSKTGWIALPYIWNKEMTDARLE
                     IVGSKQTLTYKSADGKKLNLNYIIPNTNQCLACHGDQKQFQPIGPKAKNLNRLYTYSD
                     GEENQLLKWKKLGYLTGLTDIPTTPSFPNFESIKKENLREQARAYLDVNCAHCHNPEG
                     VANTSGLMLNYEETNKNKIGFCKTPVASGKGSGNMLYDIYPGKPDQSILLYRMMSNEP
                     DIQMPEIGRSIHHKEGVELIRQWIQSEKGDCI"
     gene            complement(12865..14076)
                     /locus_tag="PLF66_03950"
     CDS             complement(12865..14076)
                     /locus_tag="PLF66_03950"
                     /inference="COORDINATES: protein motif:HMM:TIGR03805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="parallel beta-helix domain-containing protein"
                     /protein_id="HMZ63224.1"
                     /translation="MIRKLLILSLFLLVLFCKKKEIIVADADFQKKTMEALLKAKPDS
                     IIELPEGKFQLDASLSLTVNRVKIKGKGIDKTILSFKGQTNGAEGILVTADDFSIEDL
                     SIEDTVGDAIKIKGANGVTIRRVRTAWTGGPNEKNGAYGIYPVQCKNVLIEDSIASGA
                     SDSGIYVGQSTNIIVRRNKAEYNVAGIEIENSTYADVYENIATNNTGGILVFDLPDLP
                     VQGGKNTRVFKNQVFNNNTDNFAPKGNIVGVVPPGTGILVLANDNIEIFENRIENHDT
                     VNVGIVSYFITDKPINDPKYDPYPEAIYIHDNSITNAGENPRGLIVRLLALKLGKPFP
                     DILYDGIVDTKKANKGDLPQDLRICLERNGDADFANIDAEHTFKNIDRDIQKYSCSHP
                     KLNPVSINGIQ"
     gene            complement(14220..14915)
                     /locus_tag="PLF66_03955"
     CDS             complement(14220..14915)
                     /locus_tag="PLF66_03955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63225.1"
                     /translation="MRKSSRRKKQTNLNSERELPVEDDVVPTEPDRNIPQIDTTSYII
                     NWDSLNTRRKITAELRDEICDLYYAVQKDPKDQIEKLKQMLEEYSDIQIFYNYLENAY
                     RAEEEFEKANEIARITAKKFPDFLFGKISQIELYLEEKELDKVAELLQDKYDFRLLYP
                     EKATFHIQEILCFNYALGKYFALRTNPEKATAYLENIKGIDEDHVFVKQLQKFINKNS
                     GLKFYQKVLNKLK"
     gene            14985..15755
                     /locus_tag="PLF66_03960"
     CDS             14985..15755
                     /locus_tag="PLF66_03960"
                     /inference="COORDINATES: protein motif:HMM:NF015692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HMZ63226.1"
                     /translation="MLGAICGDVIGSVYEGNNIKSKQFPLFSKGTRFTDDTVLTVAIA
                     DALLHQKYFSATIHRYSNQYPNAGFGSRFKVWMKAKHPRPYGSYGNGSAMRVSPVAHA
                     FQSLDEVLKVAEKTSNVSHNHPDAVKGAQAIAASVFLAKKGSSKLEIKTYIENTFNYN
                     LNRSIDQIRPSYKFDVSAIGSVPEAIIAFLESTDYEDSIRNAISIGGDSDTIACMCGA
                     ISEAHYKMIPADIVDRVKALLPTEFIAVIDSFYEKVIK"
     gene            15752..16258
                     /locus_tag="PLF66_03965"
     CDS             15752..16258
                     /locus_tag="PLF66_03965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63227.1"
                     /translation="MNFGKIFVFLSGLSLGYFLTNLKGTVMNDYLYLFVGIVGGYFIF
                     NFIPYYFTAEEPVKAAAADGAAPPGEPLKEITVKKVFKASIFGDPLTPDEVIFDKKGI
                     TFNVKQFFGNTETFVLYSDVSGVEITEGILLASIKIKPKTRNTDIKIDNFAKSDAKLI
                     KKLILEKL"
     gene            complement(16259..16975)
                     /locus_tag="PLF66_03970"
     CDS             complement(16259..16975)
                     /locus_tag="PLF66_03970"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HMZ63228.1"
                     /translation="MKSAIVTGTSSGIGNSIAIKLLKLGYRVYGLSRTDSENLAANSD
                     FSFIECDLRNSKDIKSKIESLLKTEKSIHLLIHNAGFGLIGMHEELNYKNLEELIQVN
                     LTAPILITRLLLRELKANQGTIINISSITAKKNSPLASAYAATKAGLTQFGNSIFEEV
                     RKYNVKVCNIHPDVTKTNFYKNLSVEEEEEDKDSYLLPETIADCVEMILNQNQGTTIT
                     DITLQPQKHKIKRKKNTLVT"
     gene            complement(17016..18239)
                     /locus_tag="PLF66_03975"
     CDS             complement(17016..18239)
                     /locus_tag="PLF66_03975"
                     /inference="COORDINATES: protein motif:HMM:NF022825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3187 family protein"
                     /protein_id="HMZ63229.1"
                     /translation="MTKITYKKPCLKLDLYSRVKKRFASIWSRKPVIAGISHFKACSL
                     LIILLSFQKIAADNLVERPIENRNLYLPFIFFLEPYPATPVTLKKGSLSIDSGMALSN
                     MIDNNNAQTDYAGYTKYKPLFWSNYSDSFFGGRLTPLSYYLSEYFNYGSLEQQSHLKL
                     DAEVERFHTKLSYGLFENFEIGFEITALSYNSGIFDGGINAYHRAVAIPYPLRDIYPN
                     DQFAFSITDNHKPLVQAKPRTGLGDSVIDSKWRFLTQNNWIPTLAWVNTIKIPTGNSN
                     YYMSSGKSDLATGFSAKWNFSDFFHFLNVFFVNTNDPFYSRDVKVKPYAAASYTIGYT
                     LTEKFSAVTQIEYKGSPYHSSIQMINQSPVLWSFGFNWKIINSCSLRGNLTEDPITRT
                     VPDISFQSSILCISE"
     gene            18328..20247
                     /gene="gyrB"
                     /locus_tag="PLF66_03980"
     CDS             18328..20247
                     /gene="gyrB"
                     /locus_tag="PLF66_03980"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004756774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HMZ63230.1"
                     /translation="MSQQEATYGAEKIKILEGLEAVRKRPGMYIGTQDETGLHKMVYE
                     VVDNSVDEAMAGHCTHILIKILPGNIVEVSDNGRGIPAGIHPDKGVSTIEVVLTILHA
                     GGKFENDAYKVSGGLHGVGVSVVNALSEWLEVEVCQGGKKYYQKYFAGVPEKPVEVIG
                     DAASTGTTVRFKPDATIFTTTEFQYDMLSSRFREMAFLNKGLNLKVQDLRKEDGHSAE
                     FNYSGGIVSFVELLNESRHPIHDKVIHFESQKDGLVAEVALQYSDTYSENIFCFTNAI
                     NNSLGGTHLEGFRAALTRTLNDFLKKDTGLAKKMDSGFSGEDVREGLTAVISIKIPQP
                     QFNSQTKEKLVNAEVKGIMQTLVAERLTLYFEENPAIIKKIIEKATLAAKAREAARKA
                     RDLTRRKTVLEGGGLPGKLADCSEKDPALSELYLVEGDSAGGSAKQGRDRNTQAILPL
                     KGKILNVEKSRLDKILANEEIRTLISAMGTGIGGDEFNIDKIRYHKIMIMTDADIDGS
                     HIRTLLLTFFFRYMRPVIEKGYLYVAQPPLYLIKKGQEASYAYSDKEKDEILSKYGKD
                     AKTVIQRYKGLGEMNPEQLWETTMDPKKRVVLQVRLDDLVEAEETFTTLMGDEVGPRR
                     RFIEDNATKVMNLDL"
     gene            20291..22795
                     /gene="gyrA"
                     /locus_tag="PLF66_03985"
     CDS             20291..22795
                     /gene="gyrA"
                     /locus_tag="PLF66_03985"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011669141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HMZ63231.1"
                     /translation="MTNTEEESGSKILKFSTASRPDIAEAVKNGTKVIPVEVEDQMKD
                     AYLGYAMSVIVGRALPDVRDGLKPVHRRILHAMNERAWRSDKAYVKCAKIVGEVLGNY
                     HPHGDSSVYDALVRMVQDFSLRVPLIDGQGNFGSVDGDNAAAYRYTEARLAKMAEELL
                     RDIDKDTVNFSPNFDDTKEQPDVLPANFPNILVNGTSGIAVGMATNIPPHNLQETIAA
                     VVEVIKNPDIGIRDLIKIIKGPDFPTGATVIGGEGLLSAYTTGKGSIRIRSKVEIEET
                     SKGREVIVITEIPYQVNKKVLLEKIGELVNEKLIEGISEILDLSNRNGMRVELHIKKD
                     YNSQVILNQLFKLTQMQVSYGITMLAILNNKPKIFNLKEILHAFSEHRRDVIVRRTRY
                     ELKKAEERAHILEGLKIALDNIDEVIRIIRASKNPAEAKTGLMNAFPLTEVQTDAILD
                     MRLQRLTSLEVQKIIDELEEIRKLIADLKDILDKPARVSEIIINELQTVSDKFGTPRK
                     TEISNESIASTSFEAQDLIDDEDVVIQISEDQFIKRLPIDTFKRQKRGGKGVQGASTK
                     RDDFIKIIRIASTHDTIMLFSNRGKAFMMKVYELPVGTKEARGKSLKAIINLGPDEVI
                     TSICTFKEFTEDAILMATKNGIIKKSPLSAFANTKKNGIIAIGLDEKDQLIYVNLLKP
                     EQDILIASKNGLAVRTNLAKLRTQGRSAGGVRGMRLSEGDFVTGVTIVEKDSDLIVIT
                     EKGYGKRSEYSEFATKGRGGRGMTYLKITEKNGGSIGVASIKPTDEVIIIAQSGMIIR
                     MEAKDISVIGRSTQGVRVVNLKDDDVVVDFAVLESE"
     gene            22802..25051
                     /locus_tag="PLF66_03990"
     CDS             22802..25051
                     /locus_tag="PLF66_03990"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HMZ63232.1"
                     /translation="MHGLLAILFAFFILSCEEKKDIPKVIDPVDLNKSLSQAHIENFK
                     FEDKKDLVSHWKKNPGRFSRLDSKLKWLNTQATFNTDKKIFLNHSKDSILLNPEASVE
                     FNVEPGVYTLRFDAGILSNFYDGNGNLSIEIDGNSKPEFIVKIDKVETWASYERKIRI
                     SRSIRFRWKSENAFVFIGHPILMPDEFKNSKPNIILFVIDALRTDSLGFNGLKFPVSP
                     NIDQLAEESVIFSKAFVNANWTKPSMLSMFYSDYASNLGIGNIGFDVSPNQKQIFYKP
                     TRKNLIQALRENYYYTASIMNNVFLLDYTGVGFDLGFHELIQIGKDIDDTKKIADESI
                     DFLNRNNHFPFFLHINLNTPHGSYEPPSSVMQELQQETDPKVWYKENPTIRRYYGEIR
                     YADQELGRIINELKIRKLYDDTTILLTADHGELFSDHHTSEANGIYGVKYGHGMTHFE
                     EEIHVPYIIKPAKSIRSKILSNKVEKPVSLLSLTPTVLGLSNISYSKKDFKGVDYSHL
                     LFGETFQMEDSIFTEGRLSESVRTEKYKYIRFYPGYTNSQLSGRYLLSDELDELYDLE
                     TDGLEKQKLSKESSFFKKAETDLEKHSITTNSFHILFPAKPESDVYSGTIYIQGAIYK
                     VRNQKEFGVKIQNKRSLSFEFKGNNEQRELVIDTVQPNFEYSLSVYLNQKLERFRVGK
                     WGILNRSAIENYPQLLISNMKPNFSEKADLPWIYNDAKLTGEKEVGASSEMGEEVRQI
                     LKSWGYIHE"
     gene            25132..25617
                     /locus_tag="PLF66_03995"
     CDS             25132..25617
                     /locus_tag="PLF66_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HMZ63233.1"
                     /translation="MRLPRIIGIDPGSHRVGFSVLDRDVKTRKVNVIEYGTIEVASKI
                     QSPESLIIVNHKLNEVIEEYKPEYASVEELFYFKNQKTVAQVYESRGVILFTLAQKNL
                     VIVEPTVTQIKKGVTGSGTADKKQVKDAIQLILGIKDLTGHDDSWDAIAIAFVGLSMI
                     R"
     gene            25707..27245
                     /locus_tag="PLF66_04000"
     CDS             25707..27245
                     /locus_tag="PLF66_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010573256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HMZ63234.1"
                     /translation="MSLYKNSFTVSLLLCSLGSYSLYAGDFGDVYGAHPAANAMGNAV
                     TAIVNNSSAVYYNVAGLGRLSEGDSLAALSDKKKWEKENGIVSKPETKQTFKQSITEF
                     FKEARKDLFLNKPKERPTRNVNEVALHYSYASPQLSVSMLSIGDSAKKAIGGYQAQNT
                     NTPVGQDLSNLSDNYGGLGLTLNLNNIYDFRRTVRFGLNVIAPATGNLLTINDVNPTT
                     TRYLQYGVTNQKPTIMGGTGIEIIKDKLFAGIGFTAIAGGGGAILLKDVPLSPNTVTP
                     NQQIILQVKPIVNPTYGIAFTFGKFSGGISYRRETALAVNSLGARAQTTLLGIQLDFD
                     VSLYDLFSPRKWAYGFAYKPTDKWVFTVDINRELWSQLGYKNKFVGTEFMSRTKASYS
                     EPFHLVDVTVLRAGTEYKWNDSLRIRGGIARRPKATPDIPGNTNWIDFDRMIFTTGLS
                     YIVFPGMRFLENLKNPVVLDAVLEYQDMKSMKIYKYAPTSRNPNYSAGGHVWHFGFSA
                     TMFF"
     gene            27293..27760
                     /locus_tag="PLF66_04005"
     CDS             27293..27760
                     /locus_tag="PLF66_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004785918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HMZ63235.1"
                     /translation="MGTKYKGSSDDILSLNTFICLARAADSINSRLNAHITSYNLTIS
                     QFGILEALYHIGSMCQKELSDKILKSTANITTVIDNLEKRNLVKRIRQEADRRYITVE
                     LTEKGKKLIQEIFPEHVKHINEEMAILSKDEKLKLKNLCKIVGKKERDNITVH"
     gene            complement(27777..28223)
                     /locus_tag="PLF66_04010"
     CDS             complement(27777..28223)
                     /locus_tag="PLF66_04010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63236.1"
                     /translation="MSKNKTKYFQAFLGLLLSINLILPFSNCKTESEKAMEYKKRVVG
                     TIKTITEIAKQIDEKDGPELEKLILKLKKEIKASKTQLEESDSFKDDNSLKEACMEIC
                     DFYQEIVDGKWGLRGQPAIEKKKEDLYKNLNQKEIDFAKKFNFNLD"
     gene            complement(28317..29417)
                     /locus_tag="PLF66_04015"
     CDS             complement(28317..29417)
                     /locus_tag="PLF66_04015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63237.1"
                     /translation="MKKILTLLTVTILFLTYALVSQENTGTFLDLKQLKNNEITTDNK
                     SQVIDRSAYKDKEKFHEMMKTALKNRKSVIFKNEAGVIYTNGEYRFHLQPEENSASVA
                     YIEYQIDGGPFSTYSNPIPLEKEGVYKLTYRSTDVLGIVEKPQTYTINVDTKAPEVKA
                     SLVGEGTKPGDIQYYKPGVKLTAQATDAGAGVNLVIVNVNGEGNLPLTEDLTFTKGGD
                     YEIAVRALDNVYNLSKKTMLTFSIDDKKPTVSVKSDPKPVMINNITFCKKDSFIVADA
                     NDPESGIAKIEYSLNAPGDWKEYKPATLRVTESDVYKIYFRATDVSGNMSDTVEFSCR
                     VDFKPPQSKHNILYPNSDASGKKVEPKSDSAK"
     gene            complement(29497..29826)
                     /locus_tag="PLF66_04020"
     CDS             complement(29497..29826)
                     /locus_tag="PLF66_04020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63238.1"
                     /translation="MGYTEKLSDRQIALIQGSAFILTEHLNFSKEEAFKIISESLREE
                     LEASKVTFEFLEIGTIAYRQDFARKLVRRVERKSYEIRKVSPEQMKRSIDLFVKMLYE
                     SWMNLGK"
     gene            29930..30943
                     /gene="fliG"
                     /locus_tag="PLF66_04025"
     CDS             29930..30943
                     /gene="fliG"
                     /locus_tag="PLF66_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009433 - bacterial-type flagellum basal
                     body, C ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HMZ63239.1"
                     /translation="MLSKKTNLTGRQKAAIFLIAVGSEVSTEIFKHLREDEIEQITFE
                     IARLDKITPEDKEKVLVEFNELMMAQGFITNGGIDFARSLLEKALGNQKAIDIINRLT
                     SSLQVRPFDFVRRTDPAQLLNFIQGEHPQTIALILSYLDPNKAASILSALPHTIQAEV
                     AKRIATMDRVSPDVLREVERVLERKLSTLASEDYTTAGGIDSVVEILNNVDRGTEKTI
                     IEALEEEDPELAEEIKKRMFVFEDIVLLDDRAIQKVMREVDNTELAKALKSVDSEVQE
                     KIFKNMSKRAANLLREDMDFMGPVRLKDVEDAQQKIVNIIRKLEEAGEIVVARSGEDE
                     LIV"
     gene            30949..31797
                     /locus_tag="PLF66_04030"
     CDS             30949..31797
                     /locus_tag="PLF66_04030"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HMZ63240.1"
                     /translation="MSSPTAPELNFPLEEIQARVSFIEEKIGFIVDSVKRIQQELAIS
                     STLTDSEEKQLLGQVERDVHNALMKVITKAFPEDSVSSEEEHHQQGKNNFTWVLDPID
                     GSMNYLRKLPMYAISIGIQHRESNVAGVVIIPELNDVYKAILGQGVTKNLSRIRVSEI
                     QLLDRALLISSFPVNRKVILHEIVSDLSAFLTSGRSIRRTGSIVLDLCWLAEGRIDGL
                     WEKSVGLWDLSAVSVILKEAGATITDYKGNYTISYPSDIVISNGFIHNQIIDVLKKSR
                     QELSLN"
     gene            32167..35364
                     /locus_tag="PLF66_04035"
     CDS             32167..35364
                     /locus_tag="PLF66_04035"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HMZ63241.1"
                     /translation="MVNNEMLRKMKDYNLHLNSNEKEFDDISNLIQSLFSTSIVFIGF
                     YESDGMVFKSKKGFSESKITENNPLYNKLFSTKQPIHINDFSKDDLTKNVSFYLNDFQ
                     IHSFMSVPIFAPESVVIGMIGVMEKNPCQFTNLQLEGLTTISRNLAYLLENRYLKNQN
                     QHHPIRNQDLINVQGQIRDLGKAINETGIVAITDPKGVITYVNDEFCRVSKYNRDELI
                     GKTHKIINSKLHSKEFFQDLWNTIKAGRVWKGEIRNQTKDGEYYWVDTTIVPFIDSSG
                     EIYQYLAIRIDITKSKLLEKIMLDQNEFIQKVTDFIPGMVGYWNSDLRCAFANKAYLE
                     WFGKTRQEMIGLHIRDVLGEAIYLKNEPYIKGAISGTIQKFERAIPRPDGKVGYTWAQ
                     YIPDILDGVVRGFIVLVTDVTELKQAEFSLQAVERKLNEILESMPEGLIEINHLGEIV
                     YANKGAAKILDISREELSRKYLNSRDWKQIDVAGNSYPADQLPLALAMLEKKEVGPIG
                     LGIIDPQGVLKWLLVHAIPMLDKDNLLTGAVASFRDITERHEFQQKLIKAKEAADIAN
                     KAKSEFLANMSHEIRTPMNSILGFAEILNKKIKDEELKEYASSIMTSGKILLRLINDV
                     LDLSKIESGKYELNNSAVNIRRIFEDKKVLFSQKFESKGLELFIDIDKTLPELLVLDE
                     TRLRQVLLNLIGNAVKFTERGFISLSAKVSKWHENENTIDLIITIEDTGIGIPEMELE
                     KIFEAFTQRTGQDHNIYGGTGLGLTISRTLVEMMNGKIQVKSEVDKGTKFFISLDNIE
                     IFSEYSLNDVIEVQEIHTSFSNINFESAKILLVDDITDNRRIVKKYLEEYPELTIIEA
                     VNGKIAFEYTKEYRPDLILMDIKMPVMNGMEAIQILKSSPETNEIPIIALTASAFEHS
                     KAEIRSICNGYLQKPVSGKNLIEKILEFLKHTTSIYLNEEESVEEIKPLDIDKLKDLY
                     DTINRDFAQRIVDMFDCIDISEIRDLSRKIHTLAKEYGYTPFVQWGHRLEKSAQVFDT
                     NKISALINEFAKIKENLKTEIALNNNSNTVSDGKN"
     gene            35351..36454
                     /locus_tag="PLF66_04040"
     CDS             35351..36454
                     /locus_tag="PLF66_04040"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HMZ63242.1"
                     /translation="MEKIKREHQILIVDDNPRNVQILGKILSDQGYNIFIATNGFQAL
                     KSLEKKIPDLILLDINMPEMDGFETCIQIKHNENFAEIPIIFLTARNETDDMLKAFAI
                     GGADYITKPFNTPELLARVNQQLALKEKAEDLKQTNENNKELLRVLLHDLRNPIVAID
                     SINKIFNENPDVYVEMNDILLQATNNCLEILESVRKMYRLQDKKIDLELKDLNLSECV
                     HSSLLIFKNALLGKDIRVEVDIPSEIFVHVDANSFSNSILNNILTNAIKFSYPNSIIK
                     INAKETDLNVILSIKDFGIGIPERILNNIFDVTKTTSRIGTQGEIGTGYGMPLVKKFL
                     EMFGGSIELYSVEQPNKDHGTEAILKLPAAKES"
     gene            complement(36459..37256)
                     /locus_tag="PLF66_04045"
     CDS             complement(36459..37256)
                     /locus_tag="PLF66_04045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63243.1"
                     /translation="MNTNKKMAMAALGTPETIKQPEESTSKEIRISNIISMNEVTIFL
                     GDDHIKEVLKSNYNRLRHLFDRYNVVIQDKQDERMRLMKSFDQPIVILNREAPESTPS
                     ISVPYNEYNRMLRADKNAELFKAEICVPIKYRQFIIIGYVQVQNNKRLDLNSFNLVGL
                     AASSIKKEISDYKNHEESKEICKVIQISESEVTFLHSLNKHFSRIFQMGSIIIVDIFS
                     KETRLTTKLSIKAIKPLDKYFAVTGQYVNMSIEQYEKIESLLIPANP"
     gene            complement(37397..38665)
                     /locus_tag="PLF66_04050"
     CDS             complement(37397..38665)
                     /locus_tag="PLF66_04050"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat-containing protein kinase
                     family protein"
                     /protein_id="HMZ63244.1"
                     /translation="MNLKEFKLSDNLKSIPNELYQLAATLEILDLSGNQLSSLPDDFN
                     KFQNLKILFLSDNQFEKFPKVLAQCKNLDIIGFKNNQISQIEENSFPEKLRWLILTNN
                     KLTQIPKSIGNCSILQKLMLSGNQIYTIPSEISNCQNLELIRLAANQLIQIPEPLMSI
                     SRLSWIAIAANPCLPPITPNKSFKEKSWSDIEILEKLGSGASGDIFKARLRNSSEMIA
                     VKVFKGEITSDGYPIDEMNICLSTGEHPNIVKVIGKINQHPDRREGILLELIPDSFSK
                     VGDPPNFQTCTRDSFKNGLIYSEEEILHILLDISNALNHLHSLQILHGDLYAHNMMFN
                     RNQLRTYIGDFGAATSFSNHSKAFEKFAKLEIRAFGCLVDDLLNLRIAVNSDKPFIQK
                     LERLRNQCLDSNPYNRPNFLEIQKYLISRK"
     gene            39201..40136
                     /locus_tag="PLF66_04055"
     CDS             39201..40136
                     /locus_tag="PLF66_04055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63245.1"
                     /translation="MFIKIPILVLLLSLSLNAQESSESLNPNKKVDEDMESSVEKKVT
                     ERKGRKFFLWGYNRSAYSQSDINFEGPGYKFTLQSVNASDKPEKIDSTYINPYTFDVP
                     QFNWRWGKYITDAIYFSFGHDHMKYVMKRGQDASIYGYISPYALQEKHFYASPDILVY
                     LYLFPQTANSYSGVHLGEGVKVTPDLLKFEHTDGLNYFSADIGITHALWESADGKHAF
                     SINAAVGTGPVVLKSDVRLFGEGKNNKYNIGGYGVSGNVGFRFDFNKRYFIDTTVRGG
                     YFDLMHVQTTGRSQDRANQNFYFMEIIAAVGYNIF"
     gene            40328..41599
                     /locus_tag="PLF66_04060"
     CDS             40328..41599
                     /locus_tag="PLF66_04060"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HMZ63246.1"
                     /translation="MELLVIIILILLNGIFSMSEISLVSAKKNRLEASAKKGSHGAKV
                     ALELSSSPNRFLSTVQIGITLIGILTGIFSGENITKDLEVDIAKFEFFTPYAHPIAVG
                     FVLVSITFFSLVFGELVPKRIGLTYPESIAKLISQPMRILSIVTAPFIWLLIQTTELV
                     LKLLQISPSKENKVTEDEIKAIIREGTEDGEVQKIEQDIVERVFYIGDRRVSSLMIHR
                     SSMIFLESTFSKEQVRRTITEDLHSIYPVYDKHPDNIVGVVSLRSLFLNIDRDDFSIP
                     QLLQEPIYIMENTSAYKALEKFKKTNVHYALVADEYGMLQGIVTLDNILKALIGNVSD
                     FNQYELKFEKREDGSWIADGLYPLHDFLEIFELDELAQNYESNTLSGLILNELTHIPK
                     EGEKLKWKNFEIEILDMDGVKIDKVLIKKIA"
     gene            41801..47554
                     /locus_tag="PLF66_04065"
     CDS             41801..47554
                     /locus_tag="PLF66_04065"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HMZ63247.1"
                     /translation="MTSIPGYQITETLKEDSSFIIWRGLDSQNRPVICKVLKWESISL
                     KEKAALRNEYEILKKLEGKPFVIRAYELIEREDLHAIILEDFGGKSIRALLEERQFTL
                     AEILKIGIACSSALIEIHGSGIIHKDINPANLVLNPQNGDLRVIDFGISETISIKSLG
                     LNNPDRLEGTILYISPEQTGRMNRNIDQRSDLYSLGVTLYELCCGKVPFISDDPLEII
                     HAHIATYPPDPNFVNPNLPKTFSDIIVKLMEKIGEARYQSALGLRFDLERCLNSLIEK
                     GTIDDFAIAQRDFSSNFHIPQKLYGRQEDIISLFDAYTNAFEGARSIVLIDGLSGTGK
                     SALVHELFKILKNFQGVKTYYIAGKFDQFQKSIPYNAFNQAFNSYIAFVLSENKEYIL
                     SKKNDILKAMDGNGSVLLEIIPSLELVTGKLDKMDELDPTQAQNRLYYIFKKFLLTIC
                     NEANPIVLFIDDLQWADSGSLGLIKILLNDPDIKHLLFIGAYRTNEVLKTHPLSIIIE
                     DAIEDGNEITQLTVGNLNIHDIRTLLAETLYNKEEEVADLAELVLAKTSGNAFFVNQF
                     LRSLYEKKLIYFDDKDQTQNPKWKWDLEKIILEKITDNVVSLMTEKIVELSSEVVYLL
                     QIAACIGIRFRLQILSLVIQKNLRETFLILWEAIQKNLLVPVGEYKIFLTSNEVIGSR
                     DIDLEINFIHDRVQQSAYSLIKEDDRKKIHHQIGKIYFQELSLEDRKDRIFEIIHHLN
                     YGSDLIISQNEISDLIHLNYSTGKRAKQSGAYEASLSYFQKGLELMRRIGADSEVLKL
                     RIDFLSEAAETSWLLSQIGGMTAFSEEVLMSGKTPFEKIKVYKVLITYNVSMGNGIIA
                     LEKASEILKEFGVGINIFPNQIEIVKELIRMKFALIGKKISDLANQKLLEDKKQLAII
                     DLLSIASSPAYTASPNLLPILIFKQVYMSAKFGIAEASPFAYMSMALISCGALNDIES
                     GYEFGKLGLKLLEKNPSPEIGPKTNVVFNTFVRFWKEHLRNTLGPLLEAYKKSLDVGD
                     LEFAGIGCYIYTLHCFHTGRDLGTLIREMKNYSEILDSLNQSDRLPYHRMLHQLVAIY
                     YGSSHEPDKFRGDIYDPEEEIPVHLKRKDRTAIFTVYFCKGLAAYNFGKMKLAIDSFN
                     QALEDQEAVVASPNLPVLFFYLSLAYIGYAKETKVEKKALKNIKSNQKKLKFWSKYAP
                     ENFLHKYYLVEAELANLLGNEDKAKQLYDLAILHARKNEYINEEALAWELGGKYYSGK
                     NEILAEMFLQKAYTCYMKWGAFAKTKNLETLYPGYIRSLKGSQPYRDTSISKTHSMRK
                     STMLVTSSNRMDSLDINSILKASQALSSEIELPILITRLMRLMAENAGAEKGYLILEE
                     DGEYWLESKYGADNGDFARVKLSEFENIPSSIVNYVINSKLSIMLENATDQSAHTDDY
                     IKTLKPKSILCMPLLNRGEMTGILYLENNLIAGAFTEQSLNILSLLSSQASISIQNAR
                     FYKHLKRVNQSYERFVPREFLQLLDKKSILDVDLGQFTEREMTILFGDIRDFTQLSEE
                     MTPEENFKFINSFLRRMEPAISKNNGFIDKYIGDAIMALFKKADDALLAAIDMRSNLS
                     IYNMHREKHNYQHIRVGIGINTGDLMLGTIGGKARMDGTVISDAVNLAARIESLTKTF
                     HVPILISEFLVEKLENRNQFYIREIDRLRVKGKQKPVTMYECFNCDKQSVIDRKLESM
                     ELYIKGLNAFREENFYEAIDFFNECQVICPEDPIPVIYINRCSQMIRDRNSKQNKEKF
                     ALDEIGSRKALLIDDNAAFLEITSHVFSKKGYDVLTAESEKTALLKYENFLPDIVFTD
                     LHLSEGTSYTLIQSIRRIANSYNKNLIIVMTSADESEEIEETSRKIGADFFMKKPIAF
                     DELFQKLKNAIAESEKL"
     gene            complement(47549..48337)
                     /locus_tag="PLF66_04070"
     CDS             complement(47549..48337)
                     /locus_tag="PLF66_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012388722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HMZ63248.1"
                     /translation="MKNLKLITLYTILLFGITCATSPTGRSRILLVKDAEMNQAGTDA
                     FQEIKSKTPIETGARQNAYVKCIANAILGVINDDTGVQSWEVVVFRDETPNAFALPGG
                     KIGVHTGILPVAKTSGQLAAVMGHEVAHVLARHGAERVSDSQIVNKGMGAVGAASGNN
                     AAVMGAIGAGAKFGVMLPFSRKHESEADILGLEYMAKAGFDPKESIDLWKNMAAMGGS
                     KPMEIMSTHPSDETRMKQLNEKIPDADKLYKEALEKGKRPNCSL"
     gene            48475..48996
                     /locus_tag="PLF66_04075"
     CDS             48475..48996
                     /locus_tag="PLF66_04075"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HMZ63249.1"
                     /translation="MAKKIVFVLLGLVALILIIPAMMKSTFKVERSIEIARPAEVIFP
                     QISDFNRMKEWSIWAKNDPNQKYVVTGNPGAVGHKQEWDGKINGKGTQEITAIETNKY
                     ITTKLTFLEPNKMESVAKQVLEPTANGTKVIWSNEGNLDYPMGRLFGPFIDGMVGPDF
                     EKGLSNLKQLVEK"
     gene            49135..49587
                     /locus_tag="PLF66_04080"
     CDS             49135..49587
                     /locus_tag="PLF66_04080"
                     /inference="COORDINATES: protein motif:HMM:NF016056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DCC1-like thiol-disulfide oxidoreductase family
                     protein"
                     /protein_id="HMZ63250.1"
                     /translation="MNSVIFFDGVCNLCNASINFIIDHDKKQFFLFSSLQSNFFKVLL
                     SSNLDEYKISNQNGLEKVTPSIQSELQSLNSIVYLENKIFYTKSDAVIRIGSHLGGLF
                     SYSYFFKIFPKSFRDGIYEWIASNRYKWFGKKESCRIPTPDLKKRFLD"
     gene            complement(49759..51540)
                     /locus_tag="PLF66_04085"
     CDS             complement(49759..51540)
                     /locus_tag="PLF66_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004495340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HMZ63251.1"
                     /translation="MRDMFTRFKNSKFFNTFCYFWVGIFSTLSFAPYGISELVFIAPF
                     GLFWIEEKYRGQYKVLLKKGLLFSIVFCFCSYYWINYMLVVFGGFPFFIAIPLFLLYC
                     LIVNWKFGVFMALFSFLKKKIGRHNLILPGLCIILAEFFTWQIFPWYYGNLLAGNVIL
                     SQVVEYTGVYGLSILVFIISYPFFQYPIWIIIKSKSKKYKSLTKLYLVKTVNILALFA
                     LLGGYLYWKWSHVEPIATRQVLIIQPDAPLEFRDGRVRETMEALMNRIEKLVIEGSEN
                     EKPDLVVLPESGVPFYSTRNAPETTTYDLVYWPRFESLIFQLSNRFKTNIYFNEIDAS
                     FVDNKPSVENLRFHNSSTLFDPNGNRGEFYRKSYLLAFGEYIPLGETFPILYKIIPQI
                     GRFLPGEEQNLITYFKNKTDVKFNKPHLRWIDSSFMNMQTQREYYKENATELEPAGKF
                     LPLICYEVILPEFVRKFYKNEMPDFIVNITNDKWYGNTVESYEHLDLARLRSIEFRRW
                     MVRGTNSGTSAFVDHLGRIVNNKFTPLLSYDRYSRPVDVISSGPTFYLLYGNLLVWLF
                     IALSSILFGYKIYIKSNYKRQLTSVRG"
     gene            complement(51546..53189)
                     /gene="gpmI"
                     /locus_tag="PLF66_04090"
     CDS             complement(51546..53189)
                     /gene="gpmI"
                     /locus_tag="PLF66_04090"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HMZ63252.1"
                     /translation="MLRLVKKQNLKTTDKVLLVILDGVGYTKKDANAGNAIAGANLPT
                     LNNLWNNHSTIHIKAHGTAVGMPSDDDMGNSEVGHNVLGCGRIFDQGAKLVSNSIDSG
                     ELFQGKVWKDLVTNVKTKNSTLHFLGLLSDGNVHSHIDHLKALIEAAKKEGVKSVRIH
                     ILLDGRDVPEKSALEYVLPFEKFLSEKRDNNFDIKIASGGGRMTITMDRYEADWSMVE
                     RGWNFHVKGEGRTFKSAEEAIETFRKEDPKVIDQYLPGFVIADEKGPVGKITDGDSVV
                     FFNFRGDRAIEISLAMTAEKFDKFNRGPKPDVFYAGMMQYDGDLKLPDKYLVSPPAID
                     RTLSEYLISEKLHQFAISETQKYGHVTFFWNGNKSGYIDKSLETFQEIPSDVIPFDQA
                     PEMKAKEITDALIKALESKKYNFVRVNYANGDMVGHTGNYSATVRGLEFLDSCISRLK
                     DACDQNGYTLLITADHGNADEMYQLDKKGNVQTVGEKPVPKTSHTLNPVNLVIYDKEN
                     RWKLKDEKDAGLANIAATVLDLLGYEAPEGYRPSLLVRQ"
     gene            53471..53659
                     /locus_tag="PLF66_04095"
     CDS             53471..53659
                     /locus_tag="PLF66_04095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63253.1"
                     /translation="MIKEFRILDEANKLINSIASDSSAAVERKAMGGKPATRRKLHLN
                     VWLTQTHSLLLLSNDNVR"
     gene            complement(53661..54845)
                     /locus_tag="PLF66_04100"
     CDS             complement(53661..54845)
                     /locus_tag="PLF66_04100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63254.1"
                     /translation="MKKYILKEQKTLSTILSRLISKAVNSVHEDKRYVITKVTSEKDL
                     NNSVNITLEPMDGEEMGKPDLLVVIMKENKIEVRIKQENPYRNDLYHVIEVIIEPLER
                     SAARATIKNIKLLNLHYAPQLDLTSVLSLYGKTSMITQTIQKFQVMLETSLNQFGYFI
                     TKVRVGFYYAADTPLLNALADTKNSYFLRDAYKKQFYTERAFFNPITKFKPQNIDAIV
                     QNHLLENVKSVLIVPFFGAGNALLGYVELLSSLPNLGNEYLQNDIESPLGIGQLVNFI
                     ASRCEDFTFELELSYVKEWKMFSPKEDAVDISQDGRGLGLFLSTIKDYSFLESGCKVA
                     FSIPINKKHYLFYAGVKNVKKSKGDVPGTSMGVRIYSCDQPEGIGLMASYATQLITKN
                     IN"
     gene            complement(54994..56136)
                     /locus_tag="PLF66_04105"
     CDS             complement(54994..56136)
                     /locus_tag="PLF66_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002176861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="suppressor of fused domain protein"
                     /protein_id="HMZ63255.1"
                     /translation="MENTNSFKIIYQETNPYGALTAFLEDDGRTVYLYLQSEQNPEWK
                     IRALWIRNRIPAPNKREESDFSNGLAPILLSSEVTDDSDISEIKAEDVHFIWTEEGDG
                     IAVFIKEELHAFMPSWYGIKGVSGYSKFAKVETITAYPLGDSSHGVIVDTVERSRNFW
                     EFRSNKDSWKQIQSSRLSFLESKFGSHTKYWSADGGKFPYVGIAKFESPEYPQVVIYS
                     TVGLSGQNMPTVELFHKDYTKFSRIELIIAVRVLPGDKSESWVPHFLGELVKFPWNMN
                     KWLGAGHTISMNRRDPDALHLNFTSAILLKDLSPVRTSDIEVAKTPDMTGLTSESNQP
                     INFLFIQPINDEEAYFMRAEGSKALMNLIAEKGIGWVHNSEREGLL"
     gene            complement(56139..56381)
                     /locus_tag="PLF66_04110"
     CDS             complement(56139..56381)
                     /locus_tag="PLF66_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016546497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63256.1"
                     /translation="MKYLISPESAIDTFIKCRKENTPIPDNVIESIRNYKRWGQNSLT
                     GLLNASAYFPEILIEENMEENIYKLLAEFKQSEVHK"
     gene            complement(56564..57556)
                     /locus_tag="PLF66_04115"
     CDS             complement(56564..57556)
                     /locus_tag="PLF66_04115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63257.1"
                     /translation="MYLPRHYKQDKKKSINYPLIIFLILGITFIGGIAFKWKRIISYF
                     SGNSVQKLTKLEEKINEGINKKTFPKNLIQEYKAATLNYFDTAPIDSSASYLIAKSYY
                     YQILDRINFDSAEVIDLTSLERRKSYLEYFHAEEDLEAMYRSALRAQSLNGKFNESES
                     NKLFIYLYEVIVNRKKPTMLLGELSLLDSTKLNSDLNKTYVWMVFINSILSGNLEALE
                     NTLNINENLGESKIEFSPRAISYLKGIASYHNNDFVKALNFLRESKLGYDSITIEATK
                     FEAMIFYKQNLHEKAIALLENMYTETGSQDKKIIRLIKIILASKPNLKTKIKID"
     gene            complement(57699..58970)
                     /locus_tag="PLF66_04120"
     CDS             complement(57699..58970)
                     /locus_tag="PLF66_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004472933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HMZ63258.1"
                     /translation="MAKGNQVYILGGAQTDFQRNWSKEGKTFLSIMKEVTDDGLLAAG
                     IDYSEIQKLNKANRVAVFVGNFDAEQYANQGHLGAFLTEVNPAFNGVPGGRFEAACAS
                     GSIALDAAATKIRAEDYDVAIVVGVEIMKTVNSAVGGAFLGTAAFYEREAKGIEFPFP
                     KLFGKLADVILERYKLKEETFMDALAEISRINYANAKRNPNAQTRSWFMNKEHASARG
                     GENNMSVGGRLCITDCSQVTDGGALVVLASKKYATEYAKKRKLKLSNIPQIKGWGHRV
                     APITFTGKVEESKGNKFILPWTKATVDDAYKRAKMSVKNIDVFETHDCFTSSEYAAVS
                     AFGISAPGKEHEAIQKGVIDFNGKKPINPSGGLIGVGHPVGASGVRMMLDLYKQVTGT
                     AGGYQVKGAKNGLMLNIGGSATTNVVFIVGK"
     gene            complement(58982..60292)
                     /locus_tag="PLF66_04125"
     CDS             complement(58982..60292)
                     /locus_tag="PLF66_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HMZ63259.1"
                     /translation="MREIKTVTIVGANGSMGAGSAAIVAAFGEAKVHMLARDVEKAKE
                     GIEKAIKSVRTDTIKSRMIPGTYDKDLAKAVAESDWVFELVAESYSVKEPINAQIAKY
                     RKPGTIVSTVSSGLSIERLSKSFDADGQKHYFGTHFFNPPYKMILCELVSHKGSDPKV
                     KAELGKYLDEKLRRAVVYTNDTPAFAGNRVGFQLMNEIAQYAQKYQDKGGIALMDAIM
                     GGFTGRAMGPLATVDFVGLDVHKAIVDNLYEFTEDSAHATFKLPDYIQKLINKGDLGM
                     KSGQGLFKRTKSADGKREKFVYNIATGNYDPYPKIEIAFAKEASAKIAASDYKGAMEV
                     IKTAKGLEADLCRYFLARYISYSLSLVGEVVDTKENVDAAMGFGFNWVPASAFVDLLG
                     GVVETKKFLSSAKIEIPQVLAKANPKEQFYKLQNKLDARALFRA"
     gene            60530..60715
                     /locus_tag="PLF66_04130"
     CDS             60530..60715
                     /locus_tag="PLF66_04130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63260.1"
                     /translation="MNITQKIEDRENDWVIQLTLSSLILLPIVSWSIQKVIKRRLNAS
                     SLCLFNKGLIKHLKMIY"
     gene            complement(60844..62076)
                     /locus_tag="PLF66_04135"
     CDS             complement(60844..62076)
                     /locus_tag="PLF66_04135"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HMZ63261.1"
                     /translation="MMTNKILAVDDDEIIIEILKGTLSKKYEIISTTSSKEALKLFKE
                     DPALIVILDLNMPEISGKDLMAQIFDAGYKPVVIILTAQTDVNIAVELFKLGAYDYLV
                     KPFNPTEMMNRVDKAFEIAELRILNENIQKEREIRIEHQLNWNLFKENLIKKDSDRTD
                     SGLMTSINSSLIQGAGLGTLAPLVQMIQASSKLEGSYYLVEKDLLEMLFENTKFSNKL
                     IFMIGDIDYIINNELPKESLTISEIYKLIESTVSDLADYQNLRNNIIKLASDTFSKNN
                     RKIRINPEYFQKAIQELLFNALKFSEASSKIYILFEVVREEFHISVLNTPAKDTKEQN
                     GIPSEYQNIIFEPFFRISRLVYDAIPTLDFGLGLCFVEKIIRNHEGKIRVVNLKNSLD
                     NAGRVLVNFDLELPFEKQ"
     gene            complement(62073..64556)
                     /locus_tag="PLF66_04140"
     CDS             complement(62073..64556)
                     /locus_tag="PLF66_04140"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HMZ63262.1"
                     /translation="MQPTDISKIPRKLLINAILLYSITPIVFFLLAAINHGLEKAISY
                     FFSMEFIIVQSIIAIGSISLFVIYNRKLEFHLEQIKSSHGINTNSSISFANAYPIKMT
                     LLMGSFNLFTILLTESIAYYVGILFSFADFFYFFTTGLGLITITCNLFFYFSKIDLYP
                     IIRYAPYKPISIFNRIRIPIVSIIISVLLLTNIGLYKLQITNAFETKKEFMNLKIKST
                     SERLNFILEKMITKMESSAKNEVLRDTNSLAAKQYMSSLHGIKEDFVQLYYMSDMEGI
                     SFSSDKVSFDIKDRIYFKEFIQSGKLTFSKPVKSKVDGKNIMVIVIPILFDKQIKGLL
                     GMAFTLSKLGETLNENDSKYEYILFSKDNTILYDNKTENINKVIGKDILSEDENFSGL
                     ENLLKTESQNRERYTQIHYNGKQKIAFTYNIPINESKLVMLQNKDDFFSETNTLLIQM
                     SLFIFFIAISISFIIQIITSKFSKPILNTIHIFNKISNGDLTEKPKDYVPDEFGEILR
                     YLNKLINILSDTVSLIKISAKELENNSLTLSTGTSKLAISAKNQSVSIEESNVSLEQL
                     SKSVQKVSENALSQSESSKETFHAMEELKWKAEEVKEYAVSATQLATKTTVEAKRGND
                     LMQSAIGGMNQIDSSTKKIAEIIGLIGDISGQVNLLALNAAIEAARAGEYGKGFSVVA
                     EEIGKLADKTAQSARSIKVYIAEGLEEVSKGKEYVDKTAKALETIVENIKKNGIIIEG
                     ISKSTTFQFDSNQKVLSHIEKVMKMAFDISDSTKEQTITNKQINATVREINHQTQDVA
                     KEAEDIALISQKISEQAKSLNKQIDFFKI"
     gene            64707..66032
                     /locus_tag="PLF66_04145"
     CDS             64707..66032
                     /locus_tag="PLF66_04145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase"
                     /protein_id="HMZ63263.1"
                     /translation="MMKIYKIFMLPSLALLMSMFGLQAWTDHTLGTYPALASMPEVKN
                     AKPVQVEAFEDFLTKESQGLAQLIKEQEEFARANIQNYPPKPDSIVFDPNDKKNIRKN
                     FLMAMRLNPEVRLAYFIQELPGTNVSDKFPPENVSVYKNIEFLKRLNFKNLKVKSTVS
                     ALAVLATAADEPDYGHDIGLYEDNEVEPGKGIMTEHGKIYGFGKQPFGDKRFEYGSQA
                     PFHMGFYHEAGIIYKFGGFIKRTLPEYRLHQFLTLARFAFKTGHPYWGYRFTGWGLHF
                     VQDLTQPYHSTLVPGVGPSKLFFANAISMIGLSGAKNGLAKRVADRHTAIEHYQFEWF
                     ESIFRNKETDNKMLIAFSDMSQDSSYGEFDHDYIRNVLTKESNERASELDSRIENWEA
                     ILKFMDHNIKYEDVKDDSPAKQEIQEFLIILMKSFGSHTRNYLRAALKY"
     gene            complement(66081..67304)
                     /locus_tag="PLF66_04150"
     CDS             complement(66081..67304)
                     /locus_tag="PLF66_04150"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HMZ63264.1"
                     /translation="MNIQIMRARQKLADILVKHFVYHLKSELEKSDTLLNFITLEGIS
                     FKDLTNQMIEDVYKEFPQTKDQADPDYNFFKKWHESRSDEGVKPYNETTFKMYNQLYF
                     SRILDIRDNSVESVASLKAKVERVLGVGKSYGRDFDFGLFFSVENSMKENVREMLVDE
                     CKEDYKDLWIIFYKQSEFYTGLGYNYYQSLLANALKRSDELLLSIFPKNIAEELKQKG
                     KSEPVFIKQASVLFTDFTGFTRISEKLAPSVLIQELDYCFSNFDLITEKYNLEKIKTI
                     GDAYLCVGGAPYPNETHAVDACLAAIDFLNFIKERKSEKEKSSIEYWDIKIGIHVGPI
                     VAGVIGEKKLAYDVWGDTVNTASRMESMCEPNKINVSKEVYELTKDIFTFEYRGKFTP
                     KNKSEVDMYFLTGIK"
     gene            67549..68613
                     /locus_tag="PLF66_04155"
     CDS             67549..68613
                     /locus_tag="PLF66_04155"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HMZ63265.1"
                     /translation="MRKILLILILFCFLNADFPNSKNRYVLIQTGTLNLREKPKDGKV
                     IGSLQAGEIVTILEDAKDDITWKKIKTKSGLVGFASNEFLGYFPPEEIQKAKLIGVLG
                     GAGDKTNKMGIRVLAAHFSGQWYGFDDSADFVYLLNLIAKGKKSLDIQYAAKKVNSFQ
                     TDTLGKYGCQEFRGLEGRVKTPVSDTFEKSYIATYNFSDSKLDYTIKEDVSADITSNV
                     LKEAISVFKKNKVTTKELENITEKKIKMLSSGSGKSYLVARYAIKNGFAERHYLSLVA
                     ELGKNNSLKFIHSKFETLEEERGNYGGSYHFSGIVDIDDTGVPALLFFHIGFDSGIYE
                     LFKIGNDKIESVFMGGGDAC"
     gene            complement(68614..69900)
                     /locus_tag="PLF66_04160"
     CDS             complement(68614..69900)
                     /locus_tag="PLF66_04160"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HMZ63266.1"
                     /translation="MPTETHISVGTNPDTLKPYTVMLVDDSMIDRSLLKRFLQSENFD
                     ILHESTEGDDLLYHLKHTSKKPDLICVDLHMPKKNGLEVIKEINQSYPTVKILVISGT
                     EDKSMIQSLLQLKIHGFLKKPYNRNAIIDKMGQVLGRAGIINATDATSKTIHLSDLSI
                     PPLPTVAIKVMSFDTENPAGGSEELEKIISPDKGITTDIMKIANSAFYGRSGKVHTLR
                     DAITLLGMKTVKNLVMLKSNKQFTKNLDSPIYKKVLQELPVLTALIGFDLTNPLGLKR
                     IREEVFLAGLLQKIGMTILGLNFPTRYSELLELAITGVRDLLHVERENFKMDHIEAGA
                     KVFKLWNMPVSLQQSVGHQNFEPNAIDMMTDVVKLTRLAHILASRMLNFTIPDYDLKI
                     EQVIYESYNFSPELKEAFGEDYHDMIKDHPFFEMMA"
     gene            complement(70069..>70632)
                     /locus_tag="PLF66_04165"
     CDS             complement(70069..>70632)
                     /locus_tag="PLF66_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HMZ63267.1"
                     /translation="IEMAMPSEKAVIEKLSKTEYPDLFAKAFPADKEKLTYDNLAKAI
                     AAFERTLKTSDRFDDYLKGDLKALNAAEEKGLATFIEVGCAGCHYGATLGGNSYRKLG
                     ERNPYKTEDLGRYEVTKNEADKYFFKVPSLRNILLTGPYFHDGSIATIEDAVKKMGYH
                     QLNKELTDAEVKSIVTFFDALTDKSRK"
BASE COUNT        23117 a        11327 c        12268 g        23920 t
ORIGIN      
        1 gaatgataaa tgatctccta gaaaaatctg atttttctac aatagcggaa aaactaaatt
       61 accctctctc caaggaaatt ttattagcaa gatctatttc ctatttggat gattccttgg
      121 aagttccgag tcatctattg gatcaaatta atttgaatca aaataaaaat ataccggtca
      181 ttggattaac aggaacaggt ggagctggta aatcctcaat tacagatgaa ttaattcgcc
      241 gatttatctc tgatttccct gataaaaaaa ttggaattat ctcaatagac ccaagtaaaa
      301 aaagaacagg cggagcctta cttggtgata gaattcgaat gaattctatc tatcatccaa
      361 atgtatttat gcgatcgctc gctactagag aagcaaacct atcggttaat aaacatatat
      421 cacaaatcat aagtctgtat cgctcttttt attttgatat tgtttttatt gaaacagcgg
      481 gtatcggtca atctgattca atgattaccg atgttgcgga tattagtata tatgcaatga
      541 ctccagagtt tggtgcagcc actcagcttg agaagattga tatgttggat tatgctgatt
      601 tagttgttgt gaataaattt gatcgatttg gagcagaaga tgcgctcaga tctgtagcaa
      661 aacaatatca aagaaatcaa aaactatttg atcagaattt agaggatatg ccagtctttg
      721 gaacgatagc ttccaaattt aatgatgaag gaatgaatac tttctatctt tccttactct
      781 cgattatcca taaaaaaaca aatatcttat ttgaatcaaa gttatataaa gattctagaa
      841 acaaaaatca aagaaatcgg agtggtatta ttccgcaaag tagaacaaga tatctatctg
      901 aaattgttgc aagcattgaa gaatataatc aatttgctat cactcagtcc gagcttgcta
      961 gaaagatgtt ttgtttaaaa agttcgattg aattgctttt acccgagaag caagattgga
     1021 caggccttga tttacacttc catgagaaag ttccattcga tgaaaatcat tttgcttctt
     1081 tcggaatatc agaattactg aaactttata aatcctattt aaatgaactt catcctgaat
     1141 gcaaagtgat tttggaatca tgggagaaga aagttaaaaa ttataaatca gaatatttta
     1201 tttatcatgt cagagaaaaa gaaataaaag tagcaaacta ctccaaatca ttatccgatc
     1261 tggatattcc caaaataact cttccaagat ataaagactg gggtgaatta ctattttggt
     1321 ctctacagga gaatgttccg ggagaatttc cttttacggc tggtgtttac ccatttaagc
     1381 gagcagaaga agagcctact agaatgtttg caggagaagg tggaccggaa agaacgaata
     1441 gaaggtttca ttatattacg aaagggcaaa atacaattcg cctctctact gccttcgatt
     1501 cagtaacgtt atacggagaa aacccggatg aacgaatgga tatttacggt aaaatcggaa
     1561 actctggtgt ttccattgca tcgttagatg acatcaaaaa gctttattct ggatttaatc
     1621 tatgcgaacc ttctacttca gtttcgatga caatcaatgg acctgctcca atgatattag
     1681 ctttcttttt aaatgccgta attgatcagc aatgtgaatt atatataaaa caaaatgggc
     1741 tattaaaaga ggtagaaagt aaaattcagc aaattttcaa agaggctaat gttaataagc
     1801 cgcattactc tggcgaatta cctgataatc acaatcaatt gggattactt ttgttaggtg
     1861 taacaggtga tagagtcttg cctaaggaga tttacgataa aattaaaatg gatgctatct
     1921 caaaagtgag aggcactgtg caggctgata tattaaaaga agaccaagca cagaacactt
     1981 gtattttttc aacagacttt gcattgaagt taatgggaga tattcaggaa tattttatac
     2041 agaataaagt cagaaatttt tactcggtct caatttcagg ataccatatt gcagaggctg
     2101 gagctaaccc cattactcaa ttagctctta ctttatcaaa tgggtttacg tatattgaat
     2161 attacctttc taggggaatg aaggtagatg actttgcacc taacttatca ttcttctttt
     2221 cgaatgggat ggatccagag tatacagttc ttggaagagt tgctagacgt gtatgggcaa
     2281 aggctattcg atttaaatac aaaggctcta tacggagtca acttttgaaa tatcatatcc
     2341 aaacatctgg caggtctctt catgcacagg aaattgagtt taatgatatt cgaaccacct
     2401 tacaagcatt aaccgctctc tatgataatt gcaattctct gcatacgaat gcatttgatg
     2461 aagcaattac aacaccgaca gaagattctg taagaagagc catggcaatt cagttaatta
     2521 ttaatcgaga gatgggactt gccaaatgtg aaaatccaac ccaaggtagt ttcattgtag
     2581 aggagttaac aaatctagtt gaggagagag tcttgcaaga gtttatccag atatcagagc
     2641 gaggtggcgt tcttggggca atggaaacaa tgtatcaaag aaataaaatt caagatgaat
     2701 ccttgtttta cgaaaaccta aaacattcag gaaaattacc aattataggt gttaatacat
     2761 ttctaaatcg taaaggatct cctacaatca tacctgatga agtaattcga tcgactcaat
     2821 ttgaaaaaga tatacaaatc tcgaatgtaa gacaagtaga aacggctaac agtcattttg
     2881 cggttcatgg tatggatcaa ttgaaaaagg ccgctcgaga gaatcagaat ttgttttctt
     2941 atctaatgga agtgagtaag tttctatcct tagggcaaat gacgaatact ctttatgaga
     3001 ttggtggtca atataggaga aatatgtaga tgagtttgta tgaacatagc ttaacaactc
     3061 gccattgcga aatggactct agtatgcaca taaataatgc aaattttctg cgttatacag
     3121 aagaggctag agttcatatg atggttgctc aacaatttcc tatggaagaa gttcataagg
     3181 caaatgtaga gatgatctta tataaatcag tttcccttta caaggaacag gttcgttatc
     3241 ccgaaagttt gactgtaaaa agtaagcaaa ttcaaacaaa gaaagttaga ggaattcttc
     3301 ggcaagaaat atacaaagaa gatggctcat tgtgttttgt ttcagacgca tattgggcat
     3361 atcatgcaaa agataaatca gcggtagatg ctactattca attcacaaaa aaatttggag
     3421 ctgactcgga taacaccatt ttacctctag aatatgataa taattttaag gctagtcata
     3481 attatccgca tgtaaatgta aaaatagagg ttagacctta tgaattagat tcatttcaac
     3541 atgtgaataa tgctgtatat gctaattatt ttgagatagg aagatgggaa tttaggagga
     3601 ctgtattacc tgatttaaat tattttaaag atatggggct aacattcgtt atttacaaga
     3661 atacaattga attcataaga ccagctttcc tatttcaaga gttaaatata agaacgtggc
     3721 taatagaaat aacgcctgct cgaattattt attggcaaga aatagagggt gaaaatggaa
     3781 cgatacacgc aaattgtcgt tccgagggat gcgttattga taaagcaggt aatcccgtaa
     3841 agctaaagcc cgaaattata cgggcatata acgctttatt agtgcactaa tatcacaaat
     3901 ttgtaaaaaa aatcaagttc aacatttttc ttgtcgaaaa aaaaaacgta atacgatata
     3961 ttaacaaaag aatcgttatg aaaaaggaaa attgaatgaa actatcaata gcaaaaatca
     4021 gttctgagag agctaagcta ctttctgcta aaactccaga gcaatacatt gctctgtcaa
     4081 ttaatagtaa attacctcca ggaaagaaag ctacaataac ttcagagtgg ttggcgaaaa
     4141 ccaattatac atatgaagat atttcttatg caagaaatag agatccatat tggagaaaga
     4201 agaaatcgtt aggtagcaag cagagaaacc ttaaacgtat tagcgagttt aattataaga
     4261 agcccggttc tacaccaaag aaaacttggt ctgttaagga aatggaagag ctttttgaat
     4321 tagataagaa actagtagat agagagcttg ctaaaaaatt taaaacatct ttaccagcaa
     4381 tcaaccacat cagaagaaag tttaagatgg taagagaaat ttttgaaatc agaaagcaaa
     4441 agcctaataa aagcaaaatc gttgcttatt ctatgaaaag tgaaaaactt ttacgcaacg
     4501 aattaaattc tttatctaag taaaattaca aagattcctg ttgaattgat ttcatcattt
     4561 gacaggattt actttttcgg atactaccag atacaatttc ctcacaggaa aatttctgga
     4621 tctggtagta gcaatcccta gtagtctctt taataaattg aggatcgttt ccttttaaca
     4681 agtaaactcg actcttttta ttcttatctg cacatttttc tggagcaatc tctgacattg
     4741 cgtatgtttg taatcctttt tttaggatag aatattcctt actaatacag gaacttaact
     4801 tcttacaaat ttcttgggca atctccatgg attcttttct ccattcggga tcgttcaact
     4861 ctgcttcttt tttattttta caatttgtgt tagatagtat aagtgcaata gctagtagta
     4921 gaaaaaaatt cttcatataa cagtcctttt attctctttt acgtatcata atttcgattc
     4981 tttcttgatt ggctgcttct tctggtgtct cagctgcgga gttttttcta aagccagata
     5041 aaccctgaac ggcaatcctt gattcaggta ttccatattt ctcacttaaa aattttgcca
     5101 taacagaggc acggtaggag ttatatgccc agttgtttag atctttttta ttttcaattg
     5161 tatctagata tggaatctga actcttaaaa taatttcgat gtttagatct ttggttactt
     5221 cagctagttt agaaaataat tcatcggagt ctttggttaa tttaatattg ccttctccaa
     5281 gtgaagaagc cgggatcgtg agagttaatt cttcattgct ttgtaaatca ataataattt
     5341 tttcagatat taacaagcgt ttgacgctac tttgaaactt ttcccaaagt ctatagagag
     5401 gagatttagg aacaagatct tggtctttta ttataccatc agatccatcc aaaactaaag
     5461 tatcaccacc atcatctagt ccaaatccac ctctaataat agaagaaaca cttttcaatt
     5521 tacctaaatc tacagtggat atagaataca taacgataaa taaacccaat aaaagggtga
     5581 tcatatcggc atacgttagc aaccatcgct cgctcatatc cttttcttct ttatgcttag
     5641 ggtatcttct tttttttgat tttgtattca tttcatttgg gagcatagtt actttccaaa
     5701 attagggaaa caggagcgaa gatattcgtg ttctgtttta tctacttccg caaaaaaaga
     5761 ctcaaacagt tctctgttta caggaagaca agcatgatat ccttcttcta ttgttgcaaa
     5821 taacggatat atccctaaaa tttttgccat aaatcgcgca ggttttatta atacagcatt
     5881 tatcggtcta tgtgctaggt catagaattg tcttaaattg agtagaacaa tttctaattg
     5941 tttaaatctt tcttctgaat gtcctagttc tataaaatac tttttaaatt catctggggt
     6001 tagatcgtat ccttccgtcc aattcttctc gataatgaga tatgccattt gagtatctaa
     6061 tttatcagtt aaattattga gctctattaa tcgttccatg ctttctctcg tctcatcttt
     6121 gatcatgctt ttgattttat caaaggagct aatagctagg tcaacccatt cggcttttgc
     6181 atctaagtta tagattttgt caaaaaagta atttaccatt tgaattgtgt cagggcgctc
     6241 aaagtaattt ttatacgtta ctttaaatcg atcgacttgt gcttttacaa cttgtttctt
     6301 tgctgcaaaa atttcttctt tactataatg gatcatatca attagagaga aaccttgtcc
     6361 tctaaattcg ttagtatttt taataattga aacatcctga ttctaaatag gatacaaaca
     6421 ggagaaaaaa tagaagaata catactcggt gagttcatta tctttgaatt tgtatgaatt
     6481 tgtaaacttt tttttaaatt tatacttgag caagatcact tggcttttga ctagaagaga
     6541 tttaaactag tgtttaattg cattcgctcc taagagtttt tcaatatgtt tgaagttaca
     6601 ttttatctga tcaatttttt taattaacta gcttatgaat attctttctt tgtttataaa
     6661 agtttcaatt tcggatgcta caaaggatat catttcttgt gctttttctt ttttatatgc
     6721 acttagattt tgttgtcttt cgaatggata atatagaagt tttgaatttg ttctcatttt
     6781 tttcattttc tttaaatagt cagcattgat cctgaaacta ccaaggctta tatcatagat
     6841 tttactttct tttagttctg aaaaagtttt ttgtataaag cttttgtata tttctttcca
     6901 attttccaaa tggagaattg gatcaataca gattcgaaca ttccaaccat ctttggttgc
     6961 attctttata ttattcaatc tttgatttaa tactgctgcg cctttctcat agttctttat
     7021 aactatttct ggcgagattg tccaagcgag aataacgttc tttacgggag ttaaattctg
     7081 aattagatta tattggttgc ttttagttct ggtctcaatt aacacatttg gattttctct
     7141 tgcgaatagg atccattttg atgtatatgg aactactctt tcaaatgcga gcaggtctgt
     7201 ttcgtaagaa atacaaagat aaataggatg attatgcgat agttcccttg ttgcatcgaa
     7261 aaaatcttct aaatttacga aaacaactat atttccagaa ttaaacattc cttgaagata
     7321 gcaatattca cagttgtaga gacaatttaa aacaagggta ttgtaataaa aatctttata
     7381 cccatagtta ttcactacat tagaaccagg ataataaaag ttatctttct ttactgctag
     7441 aattagttta atagaattct tttgagatag aaaatcttga tttggtctat taaaaatatg
     7501 cttatagtct tttatttcaa gctgtatact gtcaggaaac ttttctagaa tgtcttttgt
     7561 gattgggtag ttgtaagcgt cagattcaat gtatatatgg gaaaactttc tcaaggtaaa
     7621 tcactataaa gagttgattg catggttgaa gtagtgaatt taatatcaaa atgatcgtat
     7681 tgtccgaaat ctctaatttg tttcaatgta tttccgctga gtgatgcgct agaaacaggg
     7741 actatacaat cggatggaga gaagctactt cccaaaaccg gacaacttag atatagtcca
     7801 ggagttgacg ctcctgatcc gccagaggat tgagaagata gaacagaacc atagtaggca
     7861 taaattaaat catctcggtc agttttctga ttgattgtgg ttaacttgct gttagatgca
     7921 cctgaaattt tagaatcaaa attatcccaa gctaaatctt taccaccact ggtatttgta
     7981 agaaaacttg caatatttcc aagcgttcct ttatcagatt gaaattgact acttgcccaa
     8041 ggagatccat gatacggagt tcctgtagta ataattcgtg caatataggc aggtctagta
     8101 gcttcataca atgcaaatct tgtaatcaag ccacccattg aatgcgccaa tataacaacc
     8161 gttcctgttt ctttagcaaa taacgcatct agtcttgctc taaatcgctt tccatttatg
     8221 tcaatggaat ttgaggttaa ataatcaaag ccataaatat catactgctt agttgcaact
     8281 atttgatcga atgttgtagt ctttatgaaa tctgcccaac tatcgtttat aattctagtc
     8341 ttctgttgtg ttaaagataa gctagttgag tctctgtctg tgtaatccca accatgaact
     8401 agaactattt ttttctttcc agatgtaacg ttagccgatg ttgcttctgc cactaaatct
     8461 aagttaaagg aggatccaga tttgacgagc ggatttatgt aaaaaatttt agccgcattt
     8521 tcgccattgt cagttgttct tgaaaagtaa aatgcgataa tttgttgtat tagtttttta
     8581 tttttatctt tggaggaatt tgaacaagaa ggaagaaaag aagcgatcag gaatacaaaa
     8641 atggaaaatt taaataaacc cataggatta tttgaatgac tttattgctt cttcttcact
     8701 atcaaaaatc tctagatgag tatccatacc agtcatctta aaaacttttg caacagaacc
     8761 aacaatgtta ataattttta agctgccttg gtattttttt aaacgataca tacctgtaac
     8821 tagcgttcct attccagatg aatccataaa tggaactttt aataggttga gtataatttg
     8881 gtatttacct tgcttgattt tttcatctat catatctttt atttctttcg tattatatag
     8941 gtcgacttcc cctataatat caattatttg gatagaatta ctttctcttt caatcatttc
     9001 cataggctta ccaattcaaa attcctcttt agtaaaagac cacatttata ttaatctcat
     9061 ctcagattac actgagtcaa attcttctat tagatttaat atcttcctgt agctaggatt
     9121 ttaatcaatt acttttaata gattatttaa gatcacatat aaaatagagg agataacttt
     9181 caaatacatt tgatattagt ttgtaaaata tcaaagattt taggcatacc ctaagtttga
     9241 caattattca ggactttatt gaattcctta ataaataaac tagggtatgc taggattttt
     9301 tacttaaatt gcgctgctac ctctttcgcc atttctgatt cggattactt cctcgatagg
     9361 catgataaat attttaccat cgccaatttt tccctcacca gttttcgcag actttaggat
     9421 tgcatctaca gttggtttta cgaactcatc attcactgca atctctaagc gaacttttct
     9481 aagtaggttt acttgatact catgtcctct gaagacttca gtctttcctt tttgctggcc
     9541 gtaaccttga acatcactta cagttaatct gtaaatctcg tttttagtta attcagcctt
     9601 aacctcttct aatttatgtg gttgaataat agctacaatt aatttcattt gttatctcct
     9661 tatattccat atccattttc gccatgtatt tcttggtcaa gaccatttat ttctttatct
     9721 tcatcaattc taaatccaat cgatttttcg attaagaatg caagaatgta agagatgata
     9781 aatgagtaaa cacctgttgc aactacactc acaacttgaa cctgtatttg gtgtccacga
     9841 gtaactccgt cagcaagact cattgcaaat acacccgtta gaacagctcc aaaagctcct
     9901 cctacaccat ggataccaaa agcatcaagt gtatcatcat aaccaagttt gccttttagt
     9961 aaaatagcac cgtagcaaat tggggaaaca atgaacccca taataatcgc gcttacagga
    10021 ctcacaaaac ccgctgccgg tgtaattaca accagtcctg ctactatacc tgaagcagca
    10081 cctaaagcag ttgccttttt agtatgaatc cattcaatta ataaccacat tgcgcctgcc
    10141 gcagctggag caactaatgt taccacaaaa gctctagagg ctagtccatc aatttttaat
    10201 ccagatccag cgttaaatcc aaaccatccg aaccaaagga gtccagatcc caagagtgta
    10261 taggtcatat tatttggatg agtaagagta gatggatcgc cttttctttt accaatcaca
    10321 agcgcagcgg caagacctgc aataccagag attaaatgca ctactgttcc tccagcaaag
    10381 tccaaagccc catctttaaa taaccaacca tctgctgccc agacccagtg agcaactgga
    10441 tcataaacga gagtagccca aagtaatata aatacaacat atccagatag ttttattctt
    10501 tctgctattg ctccggaaat aagtgcaggt gtaattaatg caaacattcc ctggaataaa
    10561 aagtgaacaa attttggaat gctaccttcc agtgtgtcta catcaatacc atttaaaaag
    10621 gctaaactga aatcgccgta gaaagggttt gttcctgaaa aagcaaaact ataaccaaag
    10681 attgtccact gtaaagttaa aacaatgatc gcaataaaac tatgcatcat ggtagataga
    10741 acgtttttgg attttacgat accaccataa aataaagcaa gacctggtat cataaaaaag
    10801 accagtgcgg aagacactat caaccaaacc gtatcggctt tgtcgagtgt cggtgcagcc
    10861 ggtgccggtg ccggtgccgg tgccggtgta gttgcatcct gtccattgag taaggtcgga
    10921 attacgacca ttactaaaaa caggactaga tattttaatt tatttacttt taacatcaaa
    10981 gtgcccccta aattcaattt gttaaatagt taagactatt cacatcatac attttattct
    11041 gattaaattt ctgcaataac tatgcctgaa ataaatatgt ttaaataaat ataaaaaaag
    11101 tttcatccat tgagattaac aaacatccta aatcctttga ggggtggaga gattgatttg
    11161 ttaattcaga tttgcttttt ctttttgcgc gatttgtatt ttattttctg aaaatgatat
    11221 gtacatattg cctaatcttt ctacatgatt agggagcaag gttaattcag ttttttaaga
    11281 gtttcccgaa gccactttgc ggattcaacc tttgttccat caggcagttt tacataatat
    11341 atattgccag ttagataaga tttggttgcg atctggttta taaaatcgtc caccgttttg
    11401 attctctcac cagcaacttt taattttttc tccatatgct cttttgcttc tcttccagaa
    11461 aattcctctc catttcttat aaactttaga ttagagtttg gcttatctaa ttcattgagt
    11521 aggaatttga tcttgtctga ttctgttgaa atatgtttgg tttgtgaaaa agataattgt
    11581 ggaatcagaa gaaacagaaa caaaattgaa tacatttttt ttaacatggc ttaaatacag
    11641 tctccctttt cagattgaat ccactgccga attaattcaa ctccttcttt atgatgaata
    11701 cttcttccaa tttcaggcat ttgaatatct ggttcatttg acatcattcg atagagtaag
    11761 attgactgat ctggctttcc tggataaata tcatacaaca tattcccaga accctttcct
    11821 gatgcgactg gcgttttgca aaagccaatt ttatttttat ttgtttcttc atagttcaac
    11881 ataagtcctg aagtgtttgc aacaccctct ggattatgac aatgtgcgca atttacatct
    11941 agataggctc ttgcctgctc tcgaagattc tcttttttta tactttcaaa atttggaaaa
    12001 ctaggtgttg tagggatgtc agtaagtcct gttaggtaac ctagcttttt ccattttaat
    12061 aattggtttt cttctccatc cgaataagta tagagacgat ttagattttt tgcctttgga
    12121 ccaataggct gaaactgttt ttgatctcca tggcaagcta aacattgatt tgtatttggt
    12181 atgatataat ttaaatttag tttttttcca tcagcacttt tataagttaa tgtttgtttg
    12241 cttccaacta tttctagacg agcatctgtc atttctttat tccaaatata gggaagtgca
    12301 atccaaccag tctttgaatt tactaaaaga cgagtctcca ctaattgttt tggaaaattt
    12361 gaatcttcaa ttttagctcc ttcaaaataa aacgtctttg atatcacagt tccagtagga
    12421 aaaacaaatg ttccttcttc tttgtattga gcgcttttac ccttaggcat ccaaataaaa
    12481 cgactcttgc ttgcataatc tgtaaagaga ggcatattta aatcatatgg aagaactcca
    12541 tcatttggtt ttagttctga aatgtttcca ataaataatt tccaatcaga tagtttttct
    12601 ggataaggtt cttctaagta agcatgaaca gagttagaag gcttacagta aaaaaacaaa
    12661 acaagaaata aaaaaagcga taggttcaat aaaaaagaat ttagattcat tttaattctt
    12721 aacttagcca ttcaattctc ctaaaaaaga cgatatacac ctagaatggg taatataaaa
    12781 aactattctt ttaaagtaaa taagttttta atttaaacta ggggataatc tatattaacc
    12841 gctaattcaa atagaatttt tatgtcactg aataccatta attgaaactg gatttagttt
    12901 tggatgagaa caagaatatt tttgaatatc tctatcaata ttcttaaatg tatgttctgc
    12961 atcgatattt gcaaaatctg catctccatt tctttccaaa caaatacgca agtcttgtgg
    13021 caaatcccct ttgtttgctt tttttgtatc tacaattcca tcatagagta tgtcaggaaa
    13081 aggtttacct aatttaagcg caagtagcct tacaatcaaa cctcttggat tttctcctgc
    13141 atttgtaatg ctattgtcat gaatatagat tgcttctggg tatggatcat atttcggatc
    13201 attgattggt ttatctgtta taaaataact cacaataccc acattcactg tatcgtgatt
    13261 ttctattcta ttctcaaaaa tttcaatatt atcatttgct aatacaagaa tgcctgttcc
    13321 tggtggaact actcctacaa tatttccttt tggagcaaaa ttatctgtat tgttgttgaa
    13381 tacttggttt ttaaaaacgc gagtgttttt tccgccctga acgggaagat caggtaaatc
    13441 aaatactaga attccgcctg tattattggt tgcgatattt tcataaacat ctgcataagt
    13501 agagttttca atttcgatac ccgctacatt gtattcagct ttatttcgtc taacgattat
    13561 atttgtagat tgtcctacat agattcctga atcagatgca cctgaagcaa tagaatcttc
    13621 tattaagaca tttttacatt gaacaggata aattccatat gctccatttt tctcattcgg
    13681 tccacctgtc caagcagtcc ttactcttcg aatcgttaca ccattcgcac ctttgatttt
    13741 aatagcatct ccgaccgtat cctctatact cagatcttct atggaaaaat catctgctgt
    13801 aaccaaaatt ccctcagctc catttgtttg acctttaaat gatagaatcg ttttatcgat
    13861 tcccttgcct ttgattttta cgcgattgac agtgagagat aaacttgcat ctaattgaaa
    13921 tttaccttct ggtagttcaa tgattgaatc tggttttgcc ttgagtaatg cttccatcgt
    13981 ttttttttga aaatctgcat cggcgacgat gatttctttt ttcttgcaga atagaacgag
    14041 taaaaacaga gataggatga gaagtttacg aatcatataa aatccttttt gataagacat
    14101 tgtgtagaaa ttattactct gagtttaaaa aactcagttg tagcttggag gacatctttc
    14161 caagctactc tgattcagga tgtccgatat ggttgaatcc tgactgaata tttgaaattt
    14221 tattttagct tgtttaagac tttttggtag aactttaagc cagagttttt gttgatgaat
    14281 ttttgtagtt gtttcacaaa tacatggtct tcatcaatac cttttatatt ttctagatat
    14341 gctgttgcct tttctggatt tgttctaagg gcaaagtatt tgcctaacgc atagttgaaa
    14401 caaagaattt cctggatgtg gaaagtagct ttctctggat acaaaagacg aaagtcatat
    14461 ttatcttgga gtagttcagc aactttatcc aattcttttt cttctaaata tagttcgatt
    14521 tggctgattt ttccaaatag aaaatcagga aattttttgg ctgttattct tgcgatttca
    14581 tttgcctttt caaattcttc ttctgctctg tatgcatttt ctaaataatt ataaaaaatt
    14641 tgaatgtccg aatactcttc tagcatttgt tttaattttt cgatttgatc ttttggatct
    14701 ttttgaactg cgtaatacaa atcgcagatt tcatctctaa gctctgcagt tatctttcta
    14761 cgagtgttta atgaatccca gttaataata taggatgttg tatctatttg gggaatattt
    14821 ctatctggct ctgtaggaac tacatcatct tcaacgggta attctctttc cgaattcaaa
    14881 ttggtctgtt tctttcttct acttgacttt ctcataattt cataagcttt ataaaagtat
    14941 ttttatacaa gtgttttttg gaatcataca gtcagaggta gaatatgttg ggtgctattt
    15001 gtggggatgt aattggatct gtttatgaag gcaataatat taaatcaaag caatttccct
    15061 tattttcaaa agggacaaga tttacggatg acacggtctt gactgttgca attgcagatg
    15121 ctcttttgca tcagaagtat tttagtgcaa ctattcatcg ctatagcaac caatatccaa
    15181 atgctggatt tggaagtaga tttaaagtct ggatgaaagc aaagcatcct cgtccctatg
    15241 gtagttatgg aaatggttct gcgatgcgag taagtcctgt tgcgcatgca tttcaatctt
    15301 tggatgaggt tttgaaagtt gcagagaaga cttcaaatgt atcacataac catccagatg
    15361 ctgtaaaagg ggcacaagca attgcagcat ccgttttttt agcaaaaaaa ggaagttcaa
    15421 aactggaaat taagacatac attgaaaata catttaacta caacttaaat cgaagtattg
    15481 atcagatacg tccttcttat aaattcgatg tgagtgctat tggttcagtt ccagaagcaa
    15541 ttatcgcatt tttagaatca acggattatg aagactctat tcgaaatgcg atatcgattg
    15601 gtggtgattc tgatactatc gcttgtatgt gcggtgctat ttctgaggct cattataaaa
    15661 tgattcctgc cgatattgta gatagagtta aagcattatt acccacagaa tttatcgctg
    15721 tgatagattc tttctacgaa aaggttatta aatgaatttt ggaaaaatat ttgtatttct
    15781 atcaggattg tctcttggct attttttgac caatctaaaa gggacagtta tgaatgacta
    15841 cttataccta tttgttggaa tagttggtgg ttattttatt ttcaacttta taccttatta
    15901 ttttacagca gaagagcctg tcaaagcagc agctgctgat ggagctgcgc ctcctgggga
    15961 gcctttaaaa gaaattactg tcaaaaaagt tttcaaagca agtatatttg gagatccctt
    16021 aactcctgac gaagtgattt ttgataaaaa aggaattaca ttcaatgtaa aacaattttt
    16081 tggaaatacg gaaaccttcg ttttgtatag tgatgtatcg ggagtggaaa ttacagaagg
    16141 aattttactt gctagtatta agataaaacc aaagacaaga aatacagaca ttaaaataga
    16201 taattttgca aagtccgatg caaagttaat taagaaatta attctggaga agttataatc
    16261 aagttactaa cgtatttttt ttacgcttga ttttatgttt ttgcggttgg agtgttatat
    16321 cggttatcgt tgtgccttgg ttttgattta aaatcatttc aacacaatcg gctatcgtct
    16381 ccggaagaag ataagaatct ttgtcttcct cctcctcttc tactgagaga tttttataga
    16441 aatttgtttt ggttacgtca ggatgaatat tacaaacttt tacattgtat tttctaactt
    16501 cttcaaagat agaatttcca aattgagtta agccagcttt ggtcgctgca taggcagatg
    16561 ctaaaggaga gttttttttt gcagttattg aagatatgtt gataattgtt ccttggtttg
    16621 ctttgagttc ccttaataga agtcttgtga tcaggattgg tgcggtgaga ttgacttgga
    16681 ttaattcttc tagattttta taattcaatt cttcatgcat gccaatgaga ccaaagccag
    16741 cattatgaat tagaagatgg attgattttt ctgtttttaa aagagattca attttagatt
    16801 taatatcttt cgagtttcgt aaatcacatt caataaaaga aaaatcactg ttagctgcta
    16861 aattctctga atccgttctg gagagtccat aaactctata accaagtttt aaaagtttaa
    16921 ttgcgataga gtttccaatt ccagagcttg ttcctgtaac gatagccgac ttcataaata
    16981 aatttttaat tgtttattcc ttccgtcaac aatctttatt cgctgataca tagaatactg
    17041 ctttgaaaac ttatatcagg aaccgttctc gtaattggat cttctgttag atttcctctg
    17101 agagaacaag agttgattat tttccaatta aatccaaagg accataatac aggagactga
    17161 ttgatcattt gaatgcttga atggtagggg cttcctttat attcaatttg tgttacagca
    17221 gaaaactttt ctgttagtgt ataaccaatg gtataagagg cagcagcgta tggttttact
    17281 tttacgtctc tagaataaaa tggatcatta gtatttacaa aaaaaacatt tagaaaatga
    17341 aagaaatctg aaaaattcca ctttgccgaa aaacctgtgg ctaaatcaga tttgccgcta
    17401 ctcatatagt aatttgaatt gcctgttgga attttaatcg tattgaccca agcaagagtt
    17461 ggaatccaat tgttttgtgt taggaatcgc catttgctat caatcacact atctcctaaa
    17521 ccggttcgag gctttgcttg aactaaaggt ttatgattgt cagttattga aaaagcaaat
    17581 tgatcatttg gataaatatc tctgagcgga taaggaattg ctactgctct atgataagca
    17641 ttgattcctc catcaaaaat tcctgaattg taggagagtg ctgtaatttc aaaaccaatt
    17701 tcaaaatttt caaataatcc atagcttagc ttagtatgga atctttcaac ctcagcatcc
    17761 aatttcaaat gggactgctg ttctaaacta ccataattaa aatattcgga taggtaatag
    17821 cttaaaggtg tcaatcttcc tccaaaaaag gaatcagaat aattggacca aaagagtggt
    17881 ttatattttg tataacctgc ataatctgtt tgtgcattgt tattatcaat catgttggag
    17941 agagccatac cggaatcgat actcagtgag ccttttttga gtgttactgg tgttgctgga
    18001 tagggttcaa gaaagaatat aaaaggcaga tataaatttc tattctcaat agggcgctct
    18061 actagattgt ctgctgctat tttctgaaag gaaaggagta tgattagtaa actacaggct
    18121 ttaaaatggc taattcctgc tataactggt tttcgactcc aaatagaggc aaatctcttt
    18181 ttcacacggg agtaaagatc taattttaaa caaggctttt tatatgttat tttagtcatt
    18241 ggaacttttg ctctgtcaat ggcttcttgt ttaaaataag ttgccttata gcgaaagaat
    18301 ataggattgt tatatgaggg atattttatg agtcaacaag aagcaaccta cggcgcggaa
    18361 aaaatcaaaa tcttagaagg acttgaggct gtccgaaaac gccccggtat gtatatcgga
    18421 acgcaagatg agactggtct tcataagatg gtctatgagg ttgtggataa ctcagttgac
    18481 gaagcaatgg caggtcattg cactcatatt ttaatcaaaa ttcttccagg taatattgta
    18541 gaagtttctg ataatggacg tgggattcca gctggaattc atcctgataa aggtgtttca
    18601 acaattgaag ttgtgcttac cattttacat gcaggtggaa agtttgaaaa cgatgcatac
    18661 aaagtttctg gcggtttaca cggagtaggc gttagcgttg taaacgctct ttctgaatgg
    18721 ctcgaagtag aagtttgcca aggtggaaag aaatactatc aaaagtattt tgcaggcgtt
    18781 ccagaaaagc cagttgaggt gattggggat gcagcctcca caggaactac agttcgtttt
    18841 aagccagatg caactatatt tacaactact gaatttcaat acgatatgct ctcttctcga
    18901 ttcagggaga tggcattttt aaataaagga ctcaatctaa aagtccaaga tcttagaaaa
    18961 gaagacggtc attctgctga atttaattac tcaggaggga ttgtatcttt cgtagaacta
    19021 ctcaatgaga gtcgtcatcc gatacatgat aaagtaattc atttcgaaag ccaaaaagat
    19081 ggtttagttg cagaagtagc tttgcaatac tctgatacct attctgaaaa tattttttgt
    19141 tttacaaacg cgattaacaa tagcttaggt ggaacacacc tagaaggttt tagagccgca
    19201 cttactagaa ccttaaatga cttcttaaaa aaagatactg ggcttgcgaa gaaaatggat
    19261 agtgggtttt cgggagagga tgtccgagaa gggctaaccg ctgttatctc aataaaaatt
    19321 ccacaaccac aattcaactc acaaacaaaa gaaaaacttg tgaacgcaga agtaaaaggg
    19381 atcatgcaaa ccctcgttgc agagcgacta acgctatact tcgaagaaaa tccagctatt
    19441 ataaagaaaa ttatcgagaa agcaacactc gctgcgaaag caagagaagc tgctcgaaaa
    19501 gctagagatt taacaagacg taaaaccgtt cttgaaggcg gcggtcttcc aggaaaatta
    19561 gcggattgtt ctgaaaaaga tccagctctc tcagagttgt atctggtgga gggagattct
    19621 gctggtggtt ctgcaaaaca aggtagggat cgaaatacac aggcgattct tcctctcaag
    19681 ggtaagattt taaacgttga aaaatctaga ttagacaaga tactcgcaaa cgaagaaatt
    19741 agaactttga tctctgctat gggaactggt atcggtggag atgagtttaa tatcgataag
    19801 attcgttatc ataaaatcat gatcatgaca gatgcggata tagatggttc ccatattcga
    19861 actctactcc taacgttctt ttttagatat atgcgccctg tgattgaaaa gggttattta
    19921 tatgttgcgc aacctcctct ctatttaatc aaaaaaggtc aggaagcttc ttacgcttac
    19981 tcagataagg aaaaagatga aattctttct aagtatggga aagatgcaaa gactgtaata
    20041 caacgttaca aaggtcttgg ggaaatgaat ccagagcaac tctgggagac tactatggat
    20101 ccaaagaaga gagttgtttt acaagttaga ttggatgacc ttgtagaagc agaagaaacc
    20161 tttacaactt taatgggcga tgaagtcgga cctcgtagac gcttcattga agacaatgca
    20221 acaaaagtca tgaatttaga tttataatgc cgtattaaac ggctaatagt gattaaccag
    20281 tggaatatat atgacaaata cagaagaaga atcaggttca aagatactaa aatttagcac
    20341 tgcctctaga ccagacattg cagaagcagt taagaatgga acaaaagtca ttccagtaga
    20401 agtagaagat caaatgaaag atgcatatct tggatatgca atgagtgtaa ttgtcggtcg
    20461 tgctcttccc gatgttcggg atggattaaa accggttcat agaagaattc ttcatgcgat
    20521 gaatgaaaga gcttggagat ctgataaggc ttatgtaaaa tgcgcgaaaa ttgtcgggga
    20581 agttttaggt aattatcatc cacacggaga tagttctgtt tatgatgcgt tagttcgtat
    20641 ggttcaggat ttctctcttc gggttccact cattgatggg cagggaaact tcggatctgt
    20701 ggatggagat aatgcagcag cttatcgtta tacggaagct cgtcttgcaa aaatggctga
    20761 ggaattactc agagatattg ataaagatac tgtaaatttt tctccaaact ttgatgatac
    20821 aaaagaacaa ccagatgtat tgccagcaaa ttttccaaat atacttgtaa atggaacttc
    20881 tggaatcgca gtcgggatgg cgacaaatat tcctccccac aatttacaag agacaatcgc
    20941 tgctgttgta gaagtaatta aaaatccaga tataggcatt cgtgacttaa ttaagatcat
    21001 taaaggacct gattttccaa cgggtgcaac tgtaattggc ggtgaaggac tgttatccgc
    21061 ttacaccaca ggaaaaggat caattcgaat tcgctctaag gtagaaattg aagagacatc
    21121 caaaggaaga gaagtcattg ttatcactga aattccttac caagtaaata aaaaagtttt
    21181 acttgaaaag atcggcgaac ttgtgaatga aaaactaata gaaggtattt ctgaaattct
    21241 agatttgtcc aatcgaaatg gaatgagagt tgagcttcat atcaaaaaag attacaattc
    21301 tcaagttatt ctaaatcaac tttttaaatt aacacaaatg caagtaagtt atggtattac
    21361 catgcttgct atcttaaaca ataagccaaa gatttttaat ctaaaagaaa ttttacatgc
    21421 gttttctgag catagaagag atgtaatcgt tagacgaact cgctatgaat taaagaaagc
    21481 agaagaacgt gctcatattt tagaaggatt gaaaattgct ttagataata tcgatgaggt
    21541 aattcgtatt atccgcgctt ctaaaaatcc agcagaagca aaaactggac ttatgaatgc
    21601 attcccattg actgaagtgc aaacggatgc aatcttggat atgagattac agagattaac
    21661 ttcattagaa gttcaaaaga tcattgatga attagaagaa attcgtaaac tcattgcaga
    21721 tctaaaagat attttagata aaccagctcg cgttagtgaa attataatca atgaattaca
    21781 aactgtttca gataaatttg gaactccaag aaaaactgag atcagtaatg aaagcattgc
    21841 aagcacatct tttgaagcac aagatttaat tgatgatgaa gatgtagtga ttcaaatttc
    21901 agaagatcaa tttattaaac gtctaccgat agacacattc aaacgacaaa agcgtggtgg
    21961 taaaggagtg caaggtgctt ccactaagag agatgatttt attaagatca ttcgaattgc
    22021 ttctactcat gacacaatca tgctattttc caatcgaggg aaagcattta tgatgaaggt
    22081 gtatgaacta ccggtcggaa caaaagaagc aagaggaaaa tctctaaagg caattatcaa
    22141 tttaggaccg gatgaggtga ttacttccat ttgcacattc aaagaattta cagaagatgc
    22201 aattctaatg gcaactaaaa acggaatcat aaaaaaatct ccgctgagtg catttgcgaa
    22261 tacaaaaaag aacggtatca ttgcaatcgg tttagatgag aaagatcagc ttatttatgt
    22321 gaacttacta aaaccagaac aagacatttt aattgctagt aaaaatggac tcgcagttcg
    22381 aaccaatctt gcaaaactta gaacacaggg aagatctgcc ggtggcgtta gaggtatgcg
    22441 actatcggaa ggtgattttg taacaggtgt aacgattgta gaaaaagact cagatctcat
    22501 tgtaataact gagaaaggtt atggtaaaag aagcgaatac tccgagtttg ccacaaaggg
    22561 tagaggcgga cgaggtatga cctatttgaa aatcactgag aagaatggag gttctattgg
    22621 tgttgcttcc atcaaaccaa cagatgaagt aatcatcatt gcacaatctg gaatgattat
    22681 tcgaatggaa gcaaaagata tttctgtgat tggtaggtct acccaggggg taagagtggt
    22741 taacctcaaa gacgatgatg tggtagtaga ctttgctgtt ttagaaagtg aatagattct
    22801 aatgcatggt ctccttgcta tcctctttgc attttttata ttgtcctgtg aggaaaagaa
    22861 ggatatacct aaagtcattg atccagttga cttaaataaa agtttatcac aagctcatat
    22921 tgaaaatttt aaatttgagg ataagaaaga tttagttagt cattggaaaa aaaatcccgg
    22981 aagattttct cgtcttgatt ccaagttgaa atggttaaat acacaagcta catttaatac
    23041 agataaaaag atttttctaa atcactcaaa agattctatt ttattaaatc cagaagcaag
    23101 tgttgaattc aatgtggaac ccggagtcta tacacttcgc tttgatgcag gtatcttatc
    23161 taatttctat gatggaaatg gaaatttaag tattgaaata gatggaaatt ctaaacctga
    23221 gttcattgta aaaattgaca aagtagagac atgggctagc tatgagcgga aaatacgtat
    23281 tagccgtagt attcgtttta gatggaaatc ggaaaacgct tttgttttta ttggacatcc
    23341 tattcttatg ccggatgaat ttaaaaattc taaaccaaat attattttat ttgtaattga
    23401 tgcactccga actgattcac ttggattcaa cggattaaaa tttcctgttt cccctaatat
    23461 agatcaatta gcggaagagt ctgtgatttt ttctaaggca tttgtgaatg cgaattggac
    23521 taagccttcg atgctttcta tgttttattc tgattatgca tctaatctag gcattggaaa
    23581 tataggcttt gatgtaagtc caaatcaaaa acaaattttc tataagccta cgcgtaaaaa
    23641 tttaattcaa gcgctaaggg aaaattatta ttatactgct agtattatga ataacgtttt
    23701 cttgttagat tatactggcg ttggttttga tttaggattt cacgagttga ttcaaattgg
    23761 aaaagatatt gatgatacta aaaaaatcgc agatgaatcg attgattttt taaaccgaaa
    23821 taatcatttt ccttttttct tacatattaa tttgaatacc ccacatggtt cttacgagcc
    23881 accttctagc gttatgcaag aactacagca agaaactgat ccgaaggtat ggtataagga
    23941 aaatccaacc attcgaaggt attatggaga gattcgttat gcggatcaag aattaggaag
    24001 aattataaat gaattgaaga ttcgtaaatt gtatgacgac acaacgattc tactcactgc
    24061 agatcatggg gaacttttta gtgatcatca tacatctgaa gcaaatggga tttatggagt
    24121 aaagtatggc catggaatga ctcattttga agaagaaatt catgttccct atattatcaa
    24181 accagcgaaa tcgattcgaa gcaaaatttt atctaataaa gttgaaaagc ctgtttctct
    24241 tctttctttg acaccaactg tattagggtt atctaatata tcctattcca agaaagattt
    24301 taaaggtgtt gattactcac atttactttt tggtgagaca tttcagatgg aggattccat
    24361 ttttacagaa ggaagacttt ctgaatctgt tcgaacagaa aaatacaagt atattcgttt
    24421 ttatcccggg tatacaaatt ctcaattatc aggacgttat ttgctgagtg atgagttaga
    24481 tgaattatac gatttggaaa ctgatggact agaaaagcaa aaattatcta aagaaagttc
    24541 cttttttaaa aaggcagaga cagatttaga aaagcattct attactacaa attcatttca
    24601 tattcttttt ccagcaaagc cagaatcaga tgtttactct ggaacgatat acattcaggg
    24661 agctatttat aaagttcgta atcaaaaaga atttggagtg aaaatccaaa ataaaagaag
    24721 tttatccttt gagttcaagg gaaacaatga acaaagagag cttgtgatag atacagttca
    24781 gccaaacttt gagtattcac tatctgtata tttaaatcaa aaattagaaa gattcagagt
    24841 tgggaaatgg ggaattctaa atcgaagtgc cattgaaaat tatcctcaac ttttaatttc
    24901 gaatatgaag ccgaattttt ctgaaaaagc agatctgcct tggatataca atgatgctaa
    24961 gctaacaggg gaaaaggaag taggagcgtc atccgaaatg ggagaagagg ttaggcaaat
    25021 cttaaaaagt tggggttata tacatgagtg agaaaaaatc actgaatcaa atagaagtat
    25081 tttttaaaaa tgataattcc tctagtgctt atttaatatc ctttggagaa aatgagattg
    25141 cctagaatta ttgggatcga tcctggttca caccgtgttg gattttctgt cttggataga
    25201 gatgtaaaaa ctcgtaaagt gaatgttatt gagtatggga ctattgaagt ggcttcaaaa
    25261 atccaatcac cggagagttt aatcattgtt aaccacaaac taaatgaggt tattgaggaa
    25321 tacaagcccg aatatgcaag tgtagaagaa ttattttatt ttaaaaatca aaaaacagtt
    25381 gctcaagtat atgaatcgag aggcgtcatt ttgtttactc ttgcacagaa aaatctagtc
    25441 attgtagaac caactgttac ccaaattaaa aagggtgtaa caggtagtgg gactgcagat
    25501 aaaaaacaag taaaagatgc aattcaactt atattgggga taaaagatct aacaggacat
    25561 gatgattcct gggatgcaat tgctatagca tttgtaggat tgtctatgat ccgataaaac
    25621 ttacagtttg ctgtaaaaaa aaattgagtg acaaagatat tttaattttg aatttttgaa
    25681 agaatcgttt ttgattgggg agatttatgt cattatataa aaattctttt actgtaagtc
    25741 ttttgttatg ttcactagga agttattcgc tatatgctgg agactttgga gatgtctatg
    25801 gggctcatcc tgctgcaaat gcgatgggaa atgctgtaac agctatcgtt aataattctt
    25861 ctgctgtata ttacaatgta gccggtcttg gtagactttc agaaggtgat tctcttgcgg
    25921 ctctctccga taaaaaaaaa tgggaaaaag aaaatggaat agtttctaaa ccagagacta
    25981 aacaaacatt taaacaaagc attactgaat tttttaaaga agctagaaag gacttatttt
    26041 taaataaacc aaaagaaaga cctactagaa atgtaaatga agttgctctt cattatagtt
    26101 atgcaagtcc gcagctatca gtttcgatgc tttccatcgg tgatagtgcc aaaaaagcga
    26161 taggcggata tcaagctcaa aatacgaata caccggttgg tcaagattta tctaatttat
    26221 ctgataatta tggaggatta ggtcttacat taaatttaaa taatatttat gatttcagaa
    26281 ggacagttcg atttggttta aatgtcatag ctcctgctac tggcaatcta cttactatca
    26341 acgatgtaaa tccaacgact acacgatatt tgcaatacgg agtcacaaat caaaaaccta
    26401 ccattatggg tggaacaggt attgagataa taaaagataa attatttgct ggaatcggtt
    26461 ttactgcaat tgcaggcggt ggtggtgcta ttttattgaa agatgttcct ctgtcaccta
    26521 acactgttac tcccaatcaa caaatcattt tacaggtaaa gccaattgtg aacccaacct
    26581 atggaattgc atttacattt ggaaaatttt caggtggtat atcttataga agagaaacag
    26641 cgttagccgt aaattctctg ggtgctcgtg cgcaaacgac attacttgga attcaacttg
    26701 attttgatgt ttcattgtat gatttgtttt ctcctagaaa atgggcgtat ggtttcgcat
    26761 ataaaccaac agataaatgg gtttttacag tagatataaa tcgtgaactt tggagtcagt
    26821 taggatataa aaataaattt gttggaaccg agtttatgtc tagaacaaaa gcatcctact
    26881 cggaaccgtt tcatcttgtg gatgtaaccg tgcttagagc tggaacagaa tataaatgga
    26941 atgatagtct tagaattcgt ggtggtattg caagaagacc gaaagcgact cctgacattc
    27001 ctggaaatac aaattggata gactttgata ggatgatatt tacaaccggg ctatcatata
    27061 ttgtatttcc tggaatgaga tttctggaaa acctaaaaaa tcctgtagtt ttagatgctg
    27121 tgctcgaata ccaagatatg aaatctatga aaatatataa atacgcacct acttctagaa
    27181 atccaaatta tagtgcgggt ggtcatgttt ggcattttgg tttttctgct acaatgtttt
    27241 tttaaaaaag ttttttcttg cattttaaaa atagggtcga aaatatactg ctatgggaac
    27301 taaatataaa ggatctagtg acgacatttt atcactcaat acttttattt gtttggctcg
    27361 agccgcagat tcaatcaaca gtaggctcaa tgcgcatata actagttata atttaacaat
    27421 cagtcaattt ggaattttag aagctctata tcatattggt tctatgtgtc aaaaggaatt
    27481 gagtgataaa attctaaaat ccactgctaa tatcacaact gtaattgata atttagaaaa
    27541 aagaaactta gttaaaagaa ttcgacaaga ggcggatagg cgttatataa ctgttgaatt
    27601 gaccgaaaaa ggaaaaaagt taatacaaga aatttttcct gaacatgtta agcatatcaa
    27661 tgaagagatg gcaattcttt caaaagatga aaagctaaaa ttaaaaaatc tttgtaaaat
    27721 agtcggaaaa aaagagagag acaatattac cgttcattaa tggctcacag ccatatttaa
    27781 tctaaattaa aattaaattt ttttgcaaaa tcaatttctt tttgatttag atttttgtag
    27841 agatcttctt tttttttctc tatcgctggt tgacctctaa gtccccattt cccatcaaca
    27901 atctcttggt agaagtcaca gatttccatg caagcttctt ttaatgaatt atcatcttta
    27961 aaactatcac tttcttccaa ttgtgtcttt gaagctttta tttctttttt taatttgagt
    28021 attaactttt ctaattcagg tccgtctttt tcatctattt gttttgctat ttcggttatc
    28081 gtttttatcg ttccaactac tctctttttg tattccatcg ctttttcaga ttctgtttta
    28141 cagtttgaaa atggaagaat tagattgatt gataggagta agccaagaaa agcttgaaag
    28201 tattttgttt tattttttga catattgaaa ggtagggaaa gttttttaga ttgttataaa
    28261 acaaaaaaag gaagaggttt agctcttcct tttttgttta gtcaagtagt tttatgttat
    28321 ttagcagaat ctgattttgg ttctactttt ttaccacttg catcactatt tgggtagaga
    28381 atgttatgtt tagattgtgg tggtttaaag tctacacggc aagaaaattc tactgtatcg
    28441 gacatgttac cagaaacgtc agttgctcta aaatagattt tataaacatc tgattctgtt
    28501 actcttaaag tagctggttt gtattctttc caatctcctg gagcatttaa gctatattca
    28561 attttagcaa ttcctgattc tggatcattt gcatccgcaa cgataaaaga atctttctta
    28621 cagaaagtga tattgttaat cataacaggt ttaggatcag acttaacaga aacggtaggt
    28681 tttttatcat caatagaaaa tgttaacatt gttttcttgg ataagttgta aacgttatct
    28741 aaagctctga ctgcaatttc ataatccccg cctttcgtaa atgttaagtc ctctgttaaa
    28801 ggtaaatttc cttctccatt tacattgacg attactaaat ttacaccagc ccctgcatca
    28861 gttgcttgtg cagttagttt aacacctggc ttgtagtatt gaatgtctcc tggttttgtt
    28921 ccttcaccaa caaggcttgc tttcacttca ggtgcttttg tatctacatt gatagtataa
    28981 gtttgtggct tttcgacaat acctaaaaca tctgtgcttc tgtaagttag tttatatacc
    29041 ccttcttttt ctaatggaat cggatttgaa tatgtagaga aaggacctcc atcgatttgg
    29101 tattcgatat acgctacaga agcagaattt tcttctggtt gtaaatgaaa acggtattca
    29161 ccattcgtgt aaattactcc tgcttcattt ttgaaaatta cactctttct atttttaagt
    29221 gcagttttca tcatctcatg aaatttctct ttgtctttat acgcagaacg atcgataacc
    29281 tgacttttgt tatcggtggt aatttcatta tttttgagtt gtttcaaatc gagaaatgtt
    29341 ccagtgttct cttgcgatac cagtgcatag gttaaaaata aaattgttac agtgagcaat
    29401 gttaaaatct ttttcacggt taaaccttcc tattgtgttg tgtgtttaat tgtagttgat
    29461 aaatatagag ataatatgta aagaaaaaaa gacctattat ttccctaagt tcatccaact
    29521 ttcataaagc atttttacaa atagatctat agatcgtttc atttgttctg gtgaaacttt
    29581 tctaatctcg taggactttc tttctactct tcgaacgagt ttcctcgcaa aatcctgtct
    29641 ataagcaatc gtgccaatct ctaggaattc gaacgtaact ttacttgctt ctagttcttc
    29701 tcttagagat tcggaaatga ttttgaaagc ctcttcttta gagaagttta gatgctctgt
    29761 gaggataaaa gctgacccct gaatgagtgc aatttggcga tcactgagtt tttcggtata
    29821 acccattcat caactttttt taattcaaga tagtaaatca attcttttta cgtgcttttt
    29881 ttccgtattg atttattcta agtttaccga tataaaagta gggtctcaaa tgctaagtaa
    29941 aaagacaaac ttaactggtc gtcagaaagc tgctattttc ctaattgccg ttggtagtga
    30001 agtttctact gaaattttta aacatcttag agaagatgaa attgaacaaa tcacatttga
    30061 gattgcccgt ctagataaga ttactccaga agacaaagag aaagttttag tagaattcaa
    30121 tgagcttatg atggctcaag gcttcattac gaatggtggt atagatttcg ctagatccct
    30181 actcgaaaaa gcactcggaa atcaaaaagc aattgatatc attaaccgac taacgtcttc
    30241 tctacaagtt cgaccttttg attttgttcg tagaacagat cccgcacagt tattaaactt
    30301 tatacaaggt gagcatccac aaacgattgc cttaattctt tcttatttgg atccaaacaa
    30361 agcggcaagt atactatccg cattgccaca tacaattcaa gcagaggttg ctaaacgaat
    30421 tgctactatg gaccgtgtaa gtccagatgt tctgcgtgaa gttgaaagag ttttggagcg
    30481 taagctctct acacttgctt ccgaggatta tacaactgct ggtggtattg attcagtggt
    30541 tgagatattg aataatgtgg atcgtggaac agagaaaaca attatcgaag ctttggaaga
    30601 agaagatcca gaattggcgg aagaaatcaa aaagcgtatg ttcgtattcg aggatatcgt
    30661 attactcgac gaccgtgcta ttcaaaaagt tatgcgcgaa gtggataaca cagagttagc
    30721 caaagctttg aaatcggttg attccgaagt tcaggaaaag attttcaaaa acatgtcaaa
    30781 gcgtgctgca aacctactcc gagaagatat ggattttatg ggtcctgttc gattaaaaga
    30841 tgtggaagac gctcagcaaa aaatagtaaa tattatccgc aaactagaag aagcgggaga
    30901 aatagttgtt gctcgttctg gtgaagatga actcatagtt taattactat gagttctccc
    30961 actgcacctg aattaaattt tcctttagaa gaaatccaag ctagagttag tttcatagaa
    31021 gagaaaattg gtttcattgt tgactcagtg aaacgaatac agcaggaact tgcgatttca
    31081 tccaccctaa cagattcaga agaaaagcaa ttactcggac aagtggaaag agatgtccac
    31141 aatgcattga tgaaggtaat tacaaaggca tttcctgagg attctgtttc ctcagaggaa
    31201 gagcatcatc agcaagggaa aaacaatttt acatgggttt tggatccgat tgatggttct
    31261 atgaattatc ttagaaaatt acctatgtat gcaatctcga tcgggattca gcacagagaa
    31321 tcaaatgttg ccggtgttgt cattatacca gaactaaatg atgtttataa ggcaatatta
    31381 ggacagggag tgactaagaa tttatctcga attcgtgttt ctgaaattca attactcgat
    31441 agagcattac tcatatcttc ttttcctgtt aatcgaaaag ttatcttaca cgaaattgtt
    31501 tccgacctat ctgcttttct cacatctggt agaagtattc gtagaactgg ttcgatcgtt
    31561 ctagatttat gctggttggc agaaggaaga attgatgggt tatgggaaaa gtctgttgga
    31621 ctttgggact tatctgcagt atcagttatt ttaaaagaag caggagcgac aattacagac
    31681 tacaaaggaa attatacaat ttcttatcca agtgatattg tcatttccaa tggatttatt
    31741 cataatcaaa tcatagacgt tctaaaaaaa tcaagacaag aactatcgct taattaaaat
    31801 aagttagaaa atcctatcta tatttcaaga cccaactcga attaataata ctcgtgtatt
    31861 cttattttaa aagcatcatg gattcataac taaaacgtta tatttacttc gtaataaaaa
    31921 cataaaattt taaatagaag actaacgata atgttttagt tgattttttg ttttccttcc
    31981 aacaattttt ttaatccgat gtttcttaaa taaaccgttt attgcaattt ctaatgacgc
    32041 actacctatt aaaatacctc taattaaatt tttagctttt aaaaaagaac cattgatttc
    32101 agtttaaatt attctagtaa atttgatact attccataac tttccttttt tggtttggag
    32161 ggatatttgg ttaataatga gatgctaaga aagatgaaag actataatct acatttgaat
    32221 tcaaatgaaa aagaatttga tgatatttca aatctcattc aatccctttt ttcaacttcc
    32281 attgtgttta ttggatttta tgaatctgat gggatggtat tcaaatctaa aaaaggtttt
    32341 tctgaaagta aaataacaga aaacaatccc ttatataata aacttttttc tactaaacag
    32401 ccaatacata taaatgattt ttctaaagat gatctaacaa agaatgtttc attttactta
    32461 aatgattttc aaatacattc ttttatgagc gtccctattt ttgcacctga atcagtcgtt
    32521 attggaatga ttggtgtaat ggaaaaaaac ccttgtcaat ttactaactt gcaacttgaa
    32581 gggctaacaa ctattagtcg taatcttgcc tacctacttg aaaatcgata tttaaaaaat
    32641 caaaatcagc atcatccaat acgaaatcaa gacttaataa atgtccaagg gcaaattaga
    32701 gatttaggaa aagcgataaa tgaaactgga attgtagcga taacagaccc gaaaggtgtt
    32761 atcacttatg taaatgatga attttgtagg gtttcaaaat acaacagaga cgaactaatt
    32821 ggtaagacgc ataaaattat taattccaaa ttgcattcga aagaattttt tcaagactta
    32881 tggaacacta taaaagcagg tcgtgtttgg aagggagaaa ttcgaaatca aactaaagat
    32941 ggagaatatt actgggttga tactacgata gttcctttta ttgattcgag cggtgagatt
    33001 tatcaatatt tagcaattcg aattgatatc acaaaaagta aactattaga aaagataatg
    33061 ttagatcaga atgagtttat tcaaaaggta actgatttta ttccgggtat ggttggttat
    33121 tggaattctg atttacgatg tgcttttgca aataaagcat atctagagtg gtttggtaag
    33181 actcgacagg agatgattgg tctacatata cgagatgttt taggcgaggc aatttatcta
    33241 aaaaatgaac cttatataaa aggtgcgata tccggcacaa ttcaaaaatt tgaacgagca
    33301 atacctcgtc ccgatggaaa agttggctat acttgggcac aatacattcc agatattttg
    33361 gatggagtgg ttagagggtt tatcgttttg gtaacagatg tgaccgagct gaaacaagct
    33421 gaattttctt tgcaagctgt cgagcgaaaa ttaaatgaaa ttcttgagtc tatgccggag
    33481 gggctaatag agattaacca ccttggagag attgtgtatg cgaacaaagg cgccgctaag
    33541 attctagata taagtagaga agaactctcc agaaaatatc ttaactctag agattggaaa
    33601 caaatagatg tagctggaaa ttcttatcca gcagatcagc ttccattggc acttgctatg
    33661 ttggaaaaaa aagaggtggg tcccattgga cttggaatta tcgatcctca aggcgttcta
    33721 aaatggttat tagttcacgc aattccaatg ttagataaag acaacctact cacaggggcg
    33781 gttgcaagtt ttagagatat aacagagaga catgagtttc agcaaaaatt aattaaagcg
    33841 aaagaagcag ctgatattgc gaacaaagca aaaagtgaat ttctagccaa catgagccat
    33901 gaaattagaa caccaatgaa ttcaatttta ggctttgctg aaattttaaa taaaaagatt
    33961 aaagatgaag agctgaaaga atatgcttct tctattatga cgagtggaaa aattttactg
    34021 agactcatta atgacgtatt agatttatca aaaattgaat caggaaaata tgaattaaat
    34081 aattcagctg taaatattag aagaatattc gaagacaaaa aagttctatt ttctcaaaaa
    34141 tttgaaagca aaggtttgga gttatttatc gatatagata agacattacc agaacttctt
    34201 gttttggatg aaacaaggtt aaggcaagtt ttattaaacc taattgggaa tgctgtaaag
    34261 tttacagaaa gaggttttat tagtttatct gccaaggtat caaagtggca tgaaaatgaa
    34321 aatacgatag atctgatcat aactatagag gacacaggaa taggaattcc agaaatggaa
    34381 ttagaaaaga tattcgaagc atttacgcaa agaacaggac aggatcataa tatctatggc
    34441 ggaacaggac ttggtttaac aatctctcga acgttagtag aaatgatgaa tggtaaaatt
    34501 caagttaaaa gtgaagtgga caaaggaaca aaattcttta ttagtttaga taatattgag
    34561 attttctctg aatattcact caatgatgtt attgaggttc aggaaattca tacatcattt
    34621 tcaaatataa actttgaatc tgccaaaata ttgcttgtgg atgatattac cgacaatcga
    34681 agaattgtaa aaaagtatct agaggagtat ccagaattaa caattataga agctgtaaat
    34741 ggaaaaatcg cctttgaata tacaaaagag tatagaccgg atctaattct aatggacata
    34801 aaaatgccag tgatgaatgg aatggaagcg attcagatat taaagtcttc cccggaaaca
    34861 aatgagattc ctattatagc gcttaccgca tctgcttttg agcattctaa ggctgaaatt
    34921 cgttcaattt gcaatggcta tttacaaaag cctgtaagtg gtaaaaattt aattgaaaaa
    34981 atattagaat ttttgaaaca tactactagt atttatctaa atgaagaaga atccgttgaa
    35041 gaaataaagc ctttagatat agataaacta aaggatcttt atgatacgat aaacagagat
    35101 tttgcgcaaa gaattgtcga tatgtttgat tgtatcgata ttagcgagat tagagattta
    35161 tcgagaaaaa ttcatacttt agctaaagaa tatggatata ctcctttcgt tcagtgggga
    35221 catcgattag aaaagagtgc gcaagtattt gatacaaata aaatttcagc cttgataaat
    35281 gagtttgcga aaataaaaga aaatttgaaa acggaaatag ccttaaataa taattcaaat
    35341 actgtcagtg atggaaaaaa ttaaaagaga acatcagata ctaattgtag atgacaatcc
    35401 tagaaatgtt caaattctag gaaagattct ttcggatcaa ggctataata tattcatagc
    35461 aactaacggt tttcaagcgc tcaaatctct tgaaaagaaa ataccggatc ttattttact
    35521 tgatattaat atgcctgaga tggatggatt tgagacctgt attcaaatca agcacaatga
    35581 gaactttgca gaaattccaa ttattttctt aactgcacga aatgaaacag acgacatgtt
    35641 gaaggcattt gctattggtg gtgcagatta tataaccaag ccttttaata cgccagaatt
    35701 attggcacga gtgaatcaac aacttgcgct taaagagaaa gcagaggatc taaagcagac
    35761 aaatgaaaat aataaagaac ttctacgcgt tcttttacat gatctaagaa atccaattgt
    35821 tgcaattgat tcgattaata aaatatttaa tgagaaccct gatgtctatg tggaaatgaa
    35881 tgatatttta ctacaggcga caaacaattg tctagaaata cttgaaagtg ttagaaagat
    35941 gtatcgtttg caggataaaa aaatagacct tgaattaaaa gatctaaatt tatctgaatg
    36001 tgttcattct tcattgctta tttttaaaaa tgcgctatta ggaaaagata tcagggtcga
    36061 ggtagatatc ccttctgaaa tatttgttca tgttgatgct aattcttttt ctaattcaat
    36121 tttgaataat atccttacaa atgcgattaa attttcttat ccaaattcca taattaaaat
    36181 caatgctaag gaaacagatc taaacgttat tctctctatc aaagattttg gaatcgggat
    36241 ccctgaaaga attctgaata atatttttga tgtaacaaag acgacgagta gaattggaac
    36301 acaaggtgag attggaacag gctatggaat gccgcttgtg aaaaaattct tagaaatgtt
    36361 tggtggtagt attgaactct actcagtcga gcaaccaaat aaggatcatg gaacagaagc
    36421 aatactcaaa ctacccgccg caaaagaaag ttaatttact aaggatttgc tggaatcaaa
    36481 agactttcaa ttttttcgta ttgttcaatc gacatattca catattggcc tgtgacggca
    36541 aagtatttgt ctaacggttt gatcgcttta attgataact ttgttgtaag tctagtctct
    36601 ttcgaaaaaa tatctacaat aatgattgag cccatttgga aaattctcga aaaatgtttg
    36661 ttcaaagaat gtaaaaaagt tacttcgctc tcggatattt gaatcacttt acaaatttct
    36721 tttgattctt catgattttt ataatcacta atttcttttt ttatagagga tgctgctagt
    36781 ccgactaagt taaatgaatt taggtctaaa cgtttattgt tttgaacttg aacatagcca
    36841 atgatgataa actgtctata cttaattgga acacaaattt ctgctttaaa taactctgca
    36901 tttttatcgg ctctgagcat tcgattgtat tcattatagg gaacagaaat ggatggcgta
    36961 gattcaggag cttctctatt taaaataaca ataggttgat cgaagctctt cattagacgc
    37021 attctctcat cttgcttatc ctgaattact acattgtatc tatcaaaaag atgtcttagc
    37081 cgattgtaat ttgattttag aacctcttta atatgatcat cacctaaaaa gatagtcact
    37141 tcgttcatgc tgatgatatt actgattcta atttccttac ttgtgctctc ttcgggctgt
    37201 ttaattgtct caggagttcc aagtgcggcc atagccattt tcttatttgt gttcatacgt
    37261 gtttattttt acgaggaaag agttttacaa gtcaagaaaa ttattttttt atataaatta
    37321 tcaaatgtat tttctaatac tttcgagtct tttatcgctt tgattcagga tagggatttt
    37381 ttaatttgct ttcaatctat tttcgactta ttagatattt ctgaatctct aaaaaattag
    37441 gtcgattata tggatttgaa tctaagcatt gatttcttag tctctctaat ttttgtatga
    37501 acggtttatc tgagttaacc gctattctaa gattcaataa gtcatcaact agacatccaa
    37561 atgctcttat ctctagcttt gcaaatttct caaatgcttt agaatgattt gagaaactgg
    37621 ttgcggcacc aaagtctcca atgtatgttc ttaattgatt tctattaaac atcatattgt
    37681 gtgcgtataa atcaccgtgt aaaatttgaa gcgaatgcaa atgatttaaa gcattcgaaa
    37741 tatccaataa aatatggagg atttcttctt cagaataaat gagcccattt ttaaaactat
    37801 cacgagtgca ggtttgaaaa ttaggaggat caccaacctt agagaagcta tcaggaatta
    37861 actctagtag aatcccttct cttctatctg gatgttgatt tattttacca attactttta
    37921 caatattcgg atgttcacct gtggatagac agatattcat ttcgtctatc ggatatccat
    37981 cacttgttat ttctcctttg aatactttga ctgcaatcat ttctgaagaa tttctcagtc
    38041 tagctttaaa tatatcacct gaagcaccag atcctaattt ttctaaaatt tcaatgtcac
    38101 tccatgattt ttctttaaaa gatttatttg gagtgattgg aggtaaacaa ggatttgctg
    38161 caatcgcaat ccatgaaagt ctagaaatgc tcatgagtgg ttctggtatt tggataagtt
    38221 gattcgctgc aagtctaatt aattctaagt tttgacaatt ggatatttcg cttggtattg
    38281 tatagatttg attgccagat aacattaatt tttgtagaat actacaattt ccaatgctct
    38341 ttggaatttg cgttaatttg ttatttgtca gtattaacca gcgtaatttt tcagggaatg
    38401 aattctcttc tatttgagag atttgattat ttttaaaacc gattatatct aagtttttac
    38461 attgtgctaa aacttttggg aacttttcga attgattatc tgataaaaat aaaatcttta
    38521 aattttgaaa cttattaaaa tcatctggta gactagatag ttgatttcca gataagtcta
    38581 aaatttccaa agttgcggct aattggtata attcgttggg tatacttttt aaattatctg
    38641 aaagtttaaa ttcttttaaa ttcattccat cctaatctaa cattcgaaat taaaaacttc
    38701 aatcctctct gttataagaa tacttttata aagtgagcat cctgttttct gaacaagtat
    38761 tttaaaacaa atcctaaagt ccaatgattt ctacattaga atactttcca gaatagagaa
    38821 aggaatctat aaatgaatca tcacattatt tgaatgccag tctttatata tgataaaatt
    38881 tcctctagca acactctctt gcgcaatggt tactaaaccc tttttcgttt tattaggctt
    38941 tgaatacaaa tcgcgtctgc ataaaaaaga ggaataagag aaagaagtag gttggaatgt
    39001 tttctgtatt ttaatgcaag caaattgatg taaaaataca atatataaat atggataaaa
    39061 ctatttatat atatagtaat aatatatttt ccttttgtgg tctaggtcta taggattgga
    39121 tcttagtcgc aaaatagtag tttatctata tttattgaca taaataactc tataaaaata
    39181 tagttgccac ggaaccgaaa atgtttataa aaataccaat tttagttttg ttactatccc
    39241 tatctcttaa cgcacaagaa tcttctgaat ctttgaatcc aaataaaaaa gtagatgagg
    39301 atatggagtc ttctgttgaa aaaaaagtta cagagagaaa agggagaaaa ttttttctct
    39361 ggggatacaa tagatctgcc tattcacaga gtgatattaa ttttgaaggt cctggctata
    39421 aatttacttt acaaagtgtg aatgcatcag acaaaccaga aaaaatcgat agcacctaca
    39481 tcaatccata tacttttgat gttcctcaat tcaattggcg ttggggaaaa tacatcacgg
    39541 atgcaattta tttttcattt ggtcatgatc atatgaaata tgttatgaag agaggacaag
    39601 atgcaagtat ctacggttat atttctcctt atgctttgca agagaaacat ttttatgctt
    39661 ctcctgatat attagtatat ttatatttat ttcctcaaac tgctaattct tattctggtg
    39721 tgcatttagg tgaaggtgta aaggtaactc ccgatttatt aaaatttgaa cacacggatg
    39781 gtttgaatta cttcagtgca gatattggaa ttacacacgc tctatgggaa tctgctgatg
    39841 gaaaacatgc ttttagtatt aacgctgcgg tcggaactgg tcccgttgtc ttaaagagtg
    39901 atgtaagact ctttggagaa ggtaagaata ataaatataa tattggaggg tatggtgtat
    39961 caggtaacgt tggttttcgt tttgatttta ataaaagata ctttattgat acaactgtta
    40021 ggggaggcta cttcgattta atgcatgtgc agacaacagg tagatcacaa gatcgtgcaa
    40081 atcagaattt ttattttatg gaaatcatcg cagcagttgg ttataatatt ttttaatttt
    40141 ctctaaactc taaaacatca aaagcgcatt cccctattat taaactgtta gtagaatgcg
    40201 taaaaataag taattctact cgcattctag agagatagat tgacattcca tccacttaat
    40261 ttgcaattat taaagtgaat cggtatagac aaaaattttc ctttttataa agaatgttaa
    40321 gcaagttatg gaattactag taattataat tttaatccta ctgaatggga tattttcgat
    40381 gtcggaaata tcacttgttt cagctaagaa aaatcgttta gaagcctcag ctaaaaaggg
    40441 cagtcatgga gctaaagtcg ctctagagtt gtctagttct ccaaaccgtt tcttatcaac
    40501 tgttcagatt ggaattactc taattgggat tttaactggt atattcagtg gtgaaaatat
    40561 cacaaaggat ttagaagttg atattgcaaa gtttgaattt tttacacctt atgctcaccc
    40621 tatcgcagtt ggatttgttt tagtatccat tacttttttc tcacttgtgt ttggtgaatt
    40681 ggttccaaag agaattggac taacgtatcc tgaaagcatt gcaaaattga tttcacaacc
    40741 aatgagaatt ttatctatag tgactgcacc ttttatttgg ttattgattc aaacaacgga
    40801 attagtttta aagttactgc aaatttctcc ctctaaagag aataaagtga cagaggatga
    40861 gataaaagca attatccgtg agggaactga ggatggagaa gttcaaaaaa ttgaacagga
    40921 tattgtggaa agagtttttt atattggaga tcgcagagta agttccctga tgatacatag
    40981 aagttcaatg atatttctcg aatcgacttt ctcaaaagaa caagttagaa gaactattac
    41041 agaagatctt cattcaattt atcctgttta tgataagcac ccggataata ttgtaggtgt
    41101 cgttagtctt agaagtctat ttttaaacat tgaccgagat gattttagta tacctcaatt
    41161 attacaagaa cctatttata ttatggaaaa tacttccgct tataaggcac ttgaaaaatt
    41221 taagaaaact aacgtccatt atgcacttgt tgcagatgaa tatggaatgc tacaaggaat
    41281 tgtgactctt gataatattt tgaaagcgtt aataggtaac gtatcagact ttaatcaata
    41341 tgaattaaaa tttgagaaaa gagaggatgg atcttggatt gcagacggtt tatatccgct
    41401 acatgatttt ttagagattt tcgaattaga tgaattagca caaaattatg agagtaatac
    41461 tttaagtgga ttaatattaa atgaactcac tcatatcccc aaggagggag aaaagctaaa
    41521 atggaaaaat tttgaaattg aaatccttga tatggatgga gtaaaaattg ataaagtgct
    41581 gataaaaaag atcgcttaat cccttagttt taattctctt tttatcgaat ctgatccttc
    41641 gtgcttaaat attaaactaa tcttcttatt ttgtagcaga gttgttataa actatagtca
    41701 agactcaagt taatttcttc ttgtctatat agttaattct atttttttat ttacactttc
    41761 gttttatcct cattcaatct tgtatctgaa ttaattagtt atgacttcca tccctggtta
    41821 tcaaatcact gaaacattaa aagaggattc tagctttatt atttggagag gtctagattc
    41881 ccaaaataga cccgttattt gtaaagtctt aaaatgggag tcgatttctt taaaagaaaa
    41941 ggcagctctt cgaaatgaat atgagatttt aaaaaaatta gaggggaaac cctttgttat
    42001 ccgcgcctat gaactaatag agcgagaaga tttacatgca atcattttag aggactttgg
    42061 tggaaaatca attcgtgctc tcttagaaga aaggcaattt acacttgctg aaattttaaa
    42121 aattggaatt gcttgttcct ccgcactcat cgaaattcat ggaagcggaa ttattcataa
    42181 agatattaat cctgcaaatt tagttttgaa tcctcagaat ggcgatctac gtgtaattga
    42241 ttttggaatt tctgaaacta tttctattaa atctcttggt ctgaacaatc ctgatcgctt
    42301 agaaggaacg attctttata tttcacctga acaaacgggt aggatgaatc gaaatattga
    42361 tcaaagatca gatttatatt cacttggagt tacactctat gagttatgtt gcggcaaagt
    42421 gccatttatc tcagatgatc ctctagaaat tattcacgca catatcgcaa cttatccacc
    42481 tgatcctaac tttgtaaacc caaacctacc aaaaactttt tctgatataa tcgtaaaact
    42541 aatggaaaag attggagagg caagatatca atccgctcta gggcttcgct tcgatttaga
    42601 gagatgttta aattctctaa tagaaaaagg aacaattgat gactttgcga ttgcacagag
    42661 agatttttca tctaactttc atattccgca gaaactctat ggcagacaag aggatataat
    42721 aagtctcttt gatgcctata ccaatgcttt cgaaggtgct aggagcatag ttttaattga
    42781 tggtttatcg ggaaccggaa aatctgcttt ggttcatgag ttattcaaaa tattaaagaa
    42841 ttttcaaggc gttaaaacat attatattgc aggaaaattt gatcaatttc aaaaaagtat
    42901 tccgtataac gcttttaatc aagcatttaa ttcttatata gcattcgttt tatcagagaa
    42961 caaggaatat attttatcca aaaaaaatga tatcctgaag gcaatggatg ggaatggaag
    43021 tgtcttactg gaaataatcc cttcacttga attagttact ggaaagttgg ataaaatgga
    43081 tgagctagat ccaacgcaag ctcagaatcg actatattat atttttaaaa aatttcttct
    43141 tacgatttgt aacgaagcca atccaattgt attatttatt gatgacttac aatgggctga
    43201 ttctggctcc ttaggactta ttaaaatttt attaaatgat cctgatataa aacatttact
    43261 ttttattgga gcttacagga caaatgaagt tttaaaaacg cacccacttt caattattat
    43321 tgaggatgcg attgaagatg gaaacgagat aacccaactt acggttggaa atttaaatat
    43381 acatgatatt agaactcttc tcgcagagac tttgtataat aaagaggaag aggttgcaga
    43441 tttggctgag ctagtcttag ctaaaacttc tggaaatgcg ttctttgtga atcagtttct
    43501 tcgttctctt tatgaaaaaa aattaatcta ctttgacgat aaagaccaaa ctcaaaatcc
    43561 aaaatggaaa tgggatttag aaaaaattat tctggaaaag ataacggata acgttgtatc
    43621 tttgatgaca gagaaaattg ttgagctttc gagtgaggtt gtctacttgc ttcagatagc
    43681 tgcttgtatt ggaattcgct ttcggttgca aatactatct cttgttattc aaaaaaactt
    43741 aagagaaaca tttttaattc tttgggaagc tattcagaaa aatctattag ttcctgttgg
    43801 tgagtataag atatttttaa cttcgaatga agttatagga tctagagata tagatttgga
    43861 aattaatttt atacatgatc gagttcaaca atctgcctat agtttaataa aagaagatga
    43921 tcgcaagaag attcaccatc agatcggaaa gatttatttt caggagttaa gtttagaaga
    43981 taggaaggat cgaatttttg aaattatcca tcatttgaat tatggttctg atttaattat
    44041 cagtcagaat gaaatttctg atttgataca tttaaattat tcaaccggta aaagagcaaa
    44101 gcaatcggga gcatatgagg catctctctc ttattttcag aaaggacttg agttaatgag
    44161 aaggattgga gctgactctg aagtcttaaa acttcgaatt gattttttat cagaggctgc
    44221 tgaaacaagt tggttattga gtcaaattgg tggaatgacc gcattttcag aagaagtgtt
    44281 aatgtctggg aaaactccat ttgaaaaaat taaagtctac aaggttttaa taacgtataa
    44341 cgtttcaatg gggaatggta tcattgcttt agaaaaagca agtgaaattc taaaagaatt
    44401 tggtgttggg ataaatatat ttccaaacca gattgaaatt gtaaaagaac taattaggat
    44461 gaaatttgcc ctgatcggaa agaaaatttc agatttagct aatcaaaaac tcttagagga
    44521 taagaagcaa ttagcgatca ttgatttact ttcaatagca agttctcctg cttatactgc
    44581 atcgcctaac cttttaccaa tattaatttt taaacaggtt tatatgtcag caaagtttgg
    44641 aattgcagaa gcttctccat ttgcctatat gtcaatggca ttgattagtt gtggggcgtt
    44701 aaacgatata gaatcaggtt atgaatttgg taaattaggt cttaaacttt tagaaaagaa
    44761 tccatctccc gaaattggac caaagaccaa tgttgttttt aatacgttcg ttcgtttctg
    44821 gaaggaacat ctacgaaata cattgggtcc attattagaa gcatataaaa agagtttgga
    44881 tgtgggtgat ttggaattcg caggaattgg ttgttatata tatacattgc attgttttca
    44941 tactggaagg gacttgggaa cattgattcg agaaatgaag aattatagtg aaatccttga
    45001 tagtctaaat caatctgacc gcctacctta tcatagaatg ttacatcagt tagtcgcaat
    45061 ctattatgga tcatcccatg agccagataa attcagagga gatatatatg atcctgaaga
    45121 ggaaatccct gttcacttaa aaagaaaaga tagaactgca atctttacag tttatttttg
    45181 taaaggatta gcagcctata actttggaaa gatgaagctt gcgatagata gtttcaatca
    45241 agctttggaa gatcaggaag ctgtggttgc ttcgccgaat cttccagtat tgttttttta
    45301 tctttcattg gcttatattg gttatgcgaa agaaacaaaa gttgagaaaa aagctttaaa
    45361 gaatataaaa tcaaatcaga agaaattaaa attttggagt aaatatgccc cagaaaattt
    45421 tcttcataaa tactatttgg tagaggcaga acttgcaaat cttcttggaa atgaagataa
    45481 ggcaaagcaa ctctatgatt tagcaattct tcacgctaga aaaaatgaat atataaatga
    45541 agaagcgtta gcttgggagt tgggcggaaa atactattcg ggaaaaaatg aaatcttggc
    45601 tgaaatgttt ctgcaaaaag cctatacctg ctatatgaaa tggggagcat ttgcaaagac
    45661 taaaaatctt gaaacgttat atccaggtta tatccgaagt ttaaagggtt cacagcccta
    45721 tcgagataca tcaatttcca agactcactc tatgagaaaa tctacgatgc tcgtaacatc
    45781 atcaaatcgt atggactctt tagatataaa ttcaatttta aaagcttcac aagcattatc
    45841 gagtgaaatc gaacttccta ttttaattac tagattaatg cgtcttatgg cagagaatgc
    45901 aggtgcagaa aaaggttatt tgatattaga agaggatgga gaatattggt tagagtctaa
    45961 atacggagcg gataatggtg attttgcgag agttaaactt tctgaattcg aaaatattcc
    46021 tagctcaatt gtaaactatg taattaattc caaattgtct attatgctag agaatgcaac
    46081 agatcagtca gcacatacag atgattacat taaaacccta aagccaaaat caattctttg
    46141 tatgcctctt ctcaatcgag gagaaatgac tggtattttg tatttagaaa ataacttaat
    46201 tgcgggtgca tttacagaac aaagtctgaa tatattatct cttctttctt cacaggcatc
    46261 tatttcaatt cagaatgcaa gattctataa acatttaaag agagttaacc aatcatacga
    46321 gagatttgtt ccgagagaat ttctacaact gttagataag aaaagtatat tagatgttga
    46381 tctcggacaa tttacggaaa gggaaatgac aatcttattc ggtgatatta gagatttcac
    46441 tcaattgtcc gaggaaatga ctccagagga aaattttaaa tttatcaatt catttctaag
    46501 aagaatggaa cctgcaattt ctaaaaataa tggatttata gataaataca ttggtgatgc
    46561 gataatggca ctttttaaaa aagcggatga tgccttgtta gctgcaattg atatgagatc
    46621 aaatttatcc atctacaata tgcatagaga gaaacataac tatcaacata ttcgagttgg
    46681 aattggaatt aatactggag atttaatgct tggaacgata ggtggaaagg ctcgtatgga
    46741 tggaactgta atttccgatg cagtgaacct agctgcgaga atcgaatctc taactaagac
    46801 gtttcacgtg ccaatattaa tctcagaatt tttagtagag aaactagaaa accgaaatca
    46861 gttttatatt agagaaattg atcgcttgcg tgtaaaagga aaacagaaac ctgtaacaat
    46921 gtatgagtgt tttaactgtg ataaacaaag cgtcatcgat cgtaagcttg aatcaatgga
    46981 actttatata aaaggtctaa acgcatttcg ggaagagaat ttttatgaag caatcgattt
    47041 ctttaatgag tgtcaagtaa tttgccctga ggatccaatt ccggtaattt atatcaatag
    47101 atgtagtcaa atgattagag atcgaaatag taagcaaaat aaagaaaaat ttgctttaga
    47161 tgaaattggt tcaagaaaag ccctgttgat tgatgataat gcggctttct tggaaataac
    47221 gtctcatgtt ttttctaaga aaggttatga tgttttaaca gcggaatcag aaaagacggc
    47281 tcttttgaag tatgagaatt ttttacctga tattgttttt acagatttac atctttccga
    47341 gggaactagt tatacattga ttcaatccat tcgtaggatt gcgaatagct acaataagaa
    47401 tttaataatt gttatgacat cggcagatga atcggaagaa attgaggaaa cttctagaaa
    47461 aattggagca gactttttta tgaaaaaacc gattgcattt gatgaacttt ttcaaaagct
    47521 aaaaaatgca atcgctgagt ccgaaaaatt ataaactaca gttaggtctt ttcccttttt
    47581 ctaatgcttc tttataaagt ttatcagcat ctggaatttt ttcatttaac tgtttcattc
    47641 gagtttcatc ggatggatga gtagacatga tttccattgg tttagaacct cccatggcag
    47701 ccatattctt ccaaagatca atactttcct ttggatcaaa tccagctttt gccatgtatt
    47761 cgagtcctaa gatgtctgct tcgctttcat gtttgcgcga gaatggaagc attactccaa
    47821 atttagctcc agcgcctatc gctcccatta ctgcagcgtt atttccagag gctgctccaa
    47881 cggctcccat acctttattt acgatttgag aatcagagac tctttctgct ccgtgtctag
    47941 caagaacatg tgcaacttca tgtcccataa cggcagctag ttggccactt gttttagcaa
    48001 caggaagaat tcctgtatga actccaattt tgcctcctgg caatgcaaac gcatttggtg
    48061 tctcatctcg aaatacaaca acttcccaag attgaacccc agtatcatcg ttgatcactc
    48121 caagaattgc attcgcaata catttaacat atgcattttg acgtgcacct gtttcaatag
    48181 gagttttgga tttgatttct tgaaatgcat cagtgccagc ttgattcatt tcggcgtcct
    48241 ttactaataa gattctactt cttcctgttg gggaagtagc acatgtaatc ccgaataaga
    48301 gaattgtata gagtgtaatt aattttagat ttttcataga tacaatagtt aaaaaaaaca
    48361 aatcactgga aaggaaaaaa tgatttgaaa cttgacaaaa aagaatactt aacatatata
    48421 tagtaatatc taaactgaat atgacctctg ttatattcaa aaaaggaaat ctttatggca
    48481 aaaaaaattg tttttgtcct tttaggactg gtggcgctga ttctgattat acctgcgatg
    48541 atgaaatcta cgtttaaagt tgaacgttcg attgaaattg ctcgacccgc ggaagttatt
    48601 tttcctcaaa tttcagactt taataggatg aaggaatgga gtatttgggc aaaaaatgat
    48661 cccaatcaaa agtatgtagt aactggaaat ccaggagcag ttggtcataa gcaagaatgg
    48721 gatggaaaga tcaatggaaa aggaacacag gagataacag ccattgaaac taataaatac
    48781 attacaacca aacttacatt tctagagcca aataaaatgg aatcagttgc taagcaagtt
    48841 ttagagccta cagccaatgg aacaaaggta atctggagca atgagggtaa tctagattat
    48901 ccgatgggac gactttttgg accttttatt gatggcatgg ttggtccgga ttttgagaaa
    48961 ggactttcta acttaaaaca actagtggaa aaataagagc ctttaaaatt gaggttgtgt
    49021 aaaataagaa aaagattaat ttgaatttat gatattacag gctattgtag atgtattgaa
    49081 aaattcagga atcatttcga aaatttgtat tttacataac ccaatttttt aaaaatgaat
    49141 tcagtaattt tctttgatgg tgtttgtaat ttatgtaatg cttctatcaa ttttataatt
    49201 gatcacgata aaaaacaatt tttcctattt agttccctcc agtctaattt ttttaaagta
    49261 ttgctttctt ccaatttgga tgagtataaa atctctaatc aaaacggatt agaaaaagta
    49321 accccttcta tacaatcaga acttcaatct ttgaattcaa ttgtttatct tgaaaacaaa
    49381 atattctata cgaaatcgga tgcggtgatt cgaattggtt ctcatctagg aggtttattt
    49441 tcctattctt atttttttaa aatatttcca aagtcgtttc gagatggaat ctatgaatgg
    49501 attgcttcaa atagatataa gtggtttgga aaaaaggaat cgtgtaggat tccgactcct
    49561 gatttaaaaa aaagatttct agattaaatc cttatcagat ttggaaaatg cgaataaatc
    49621 aataaaatca agttcggcag aactctcatc gttacatctc cttcgcccca cttacgccaa
    49681 ttgccatttt aatgacaatg ggagaaaatc tcttcagaaa gcttctctct gcttatataa
    49741 agcaagctta aatttaactt agccacgaac tgacgttagt tgccgcttat agttagattt
    49801 aatataaatc ttatatccaa agagaatcga ggataatgca ataaataacc aaactaataa
    49861 atttccatac aataaataaa aagtaggtcc tgatgatatc acgtcgacgg gtcgcgaata
    49921 tcgatcatag ctcaaaaggg gagtgaattt attgttcaca attcttccta aatgatcaac
    49981 aaaagcagaa gtgcctgaat ttgttcctcg aaccatccaa cgtctaaatt caatagaacg
    50041 aagtcttgcc aaatcaagat gttcgtaact ttcaactgtg tttccatacc atttatcgtt
    50101 tgtgatattt acaataaaat caggcatttc atttttataa aactttcgga caaattccgg
    50161 tagaattact tcgtaacaaa tgagaggtaa aaattttcca gcaggctcta gttctgtagc
    50221 attttcttta taatactctc tttgtgtctg catattcata aaactagagt caatccatct
    50281 taaatgaggt ttgttaaatt taacatctgt cttattttta aaatatgtga taagattttg
    50341 ctcttcgccc ggaagaaatc ttccgatttg tggaatgatt ttatataaga ttggaaatgt
    50401 ttcacctaac ggtatatatt ctccgaaggc aagtaaatac gattttctat aaaattcacc
    50461 tctatttcca tttgggtcaa aaagtgtaga actattatga aagcgtaaat tttcaacgct
    50521 aggtttatta tcaacaaagg aagcatcgat ttcattgaaa taaatatttg ttttaaaacg
    50581 gttgcttagt tgaaaaatta atgattcaaa cctaggccaa taaactagat catatgtggt
    50641 tgtctcggga gcattgcgag tggaataaaa aggaactccg gattcaggaa gaacgactaa
    50701 atctggtttt tcattttcag aaccttcgat cactaatttt tcgattcggt tcatcaaagc
    50761 ttccattgtt tctcgaactc taccatctct gaattcaaga ggagcatctg gttggatgat
    50821 caaaacttgt cttgttgcaa ttggctcaac atgactccat ttccaatata aataccctcc
    50881 aagtaaggcg aataacgcta atatatttac tgtctttact aaataaagct ttgtgagtga
    50941 tttatatttt tttgactttg atttgataat gatccaaatt ggatattgaa agaaaggata
    51001 tgatataata aatactaaga ttgatagacc ataaacacca gtgtattcta caacttgcga
    51061 taaaataaca ttacccgcta atagattccc ataataccaa ggaaaaattt gccatgtaaa
    51121 aaattctgcc agtataatac ataagccagg aagaattaag ttgtgccttc caattttctt
    51181 ttttagaaaa ctgaataaag ccataaaaac accaaacttc caatttacaa ttaagcagta
    51241 gagcaagaaa agaggaatag ctatgaaaaa aggaaaacct ccaaagacaa ctaacatata
    51301 gttgatccag taataggagc aaaaacaaaa aacaatggaa aagagtaatc ctttttttaa
    51361 tagaactttg tattgtcccc gatacttttc ttctatccag aagagtccaa agggagcaat
    51421 aaaaactaac tcactgatac cataaggagc aaaagatagc gttgaaaaaa tacctaccca
    51481 aaaataacag aatgtattaa aaaatttgga atttttaaaa cgcgtaaaca tatcgcgcat
    51541 atcgtctatt gccttactag taaagaaggt ctatatcctt ctggagcttc atacccaagt
    51601 aaatctaaaa cagtagcagc aatatttgca agacccgcat ctttctcgtc ttttaatttc
    51661 catctattct ctttatcata gataacgaga tttacaggat ttaaagtatg acttgttttt
    51721 gggacaggct tttctcctac cgtttgaaca ttgccttttt tatccaattg atacatctca
    51781 tctgcatttc catgatctgc tgtaatcaag agtgtatatc cattttggtc acaagcatct
    51841 tttaaacgtg aaatacaact atctaaaaac tctaggcctc ttactgttgc agaataattt
    51901 cctgtatgac cgaccatatc gccgtttgca taatttactc gaacaaaatt atattttttt
    51961 gattctaaag cttttatcaa ggcatctgtt atctcttttg ctttcatttc aggagcttga
    52021 tcaaatggaa taacgtcaga cggtatttct tgaaaagttt ctaagctttt atctatatat
    52081 ccacttttat taccattcca gaaaaatgtg acgtgcccat atttttgtgt ctctgaaatt
    52141 gcaaattgat ggagtttctc actgattaga tattcgctta atgttctatc gattgcaggt
    52201 ggggaaacga gatacttatc gggaagtttt aagtcgccat catattgcat cattcctgca
    52261 taaaaaacat ctggtttagg tcctctgtta aatttgtcaa atttctcagc tgtcatcgcg
    52321 aggcttatct caatggctct gtctcctctg aaattaaaaa agacgactga atctccatca
    52381 gttatttttc cgacaggacc tttttcgtct gctataacaa agcccggaag gtattgatcg
    52441 atgactttgg gatcttcttt tctaaaggtt tcgattgctt cttctgctga tttaaatgtt
    52501 ctaccttcgc cttttacatg gaaattccaa cctctttcaa ccatcgacca gtctgcctca
    52561 taacgatcca tggttattgt catacgacca ccgccggatg caatttttat gtcaaagtta
    52621 ttatctcttt tttcggataa gaatttctcg aaaggaagga catactctag tgcagatttt
    52681 tcaggcacat ctcttccatc taataaaata tgaatacgaa cggatttaac accttctttc
    52741 tttgctgctt caattaaggc ttttaaatga tcaatatggc tatgaacatt tccgtcagat
    52801 aaaagaccta aaaaatgtaa tgttgagttt tttgttttta cgttagttac tagatctttc
    52861 catactttgc cttgaaataa ctcaccagaa tctattgagt tactgacaag cttggctcct
    52921 tgatcaaaaa tgcgaccaca accaagaaca ttgtgaccaa cttcggaatt tcccatgtca
    52981 tcatctgatg gcattccgac tgctgttcca tgtgctttta tatgaattgt agaatgattg
    53041 ttccaaaggt tatttaatgt tggaagattt gcaccagcga tcgcattgcc tgcatttgca
    53101 tcttttttgg tatatcctac accatctaga atgactaata ggactttgtc agttgttttt
    53161 aaattttgct tttttactaa tcgtaacata aatacctcaa ctcattcaag gatgatttta
    53221 tttcgagata tatgtcaacg ggaaaatctg tgatttaatc tagattctga aaccagtttc
    53281 gcattgattt attagagatt gctaatgttt ggcaatagac agtaacaaaa aatgaattct
    53341 aatttgaata tactttttct tattaatgag taagatatat ttcattctac tcttaaactt
    53401 aaaaggtatt atacattcga gtaaagaaaa ttaattactg accttacgca aaagatagaa
    53461 attgataagt atgataaagg aatttagaat attagatgaa gctaataaat taataaattc
    53521 aatagcctcc gatagctcgg ctgctgtaga gcgaaaagcg atgggaggaa agcccgccac
    53581 taggcgcaaa cttcatttga acgtttggct cacccaaact cactcattgc ttttgttaag
    53641 caatgataat gtaaggtgag ttaattaata ttcttcgtta taagttgagt tgcataagag
    53701 gccattaagc caataccttc cggctgatca caactgtaga ttcgaactcc catacttgtt
    53761 ccagggacat cacccttaga ttttttaacg ttttttacac cagcataaaa taagtagtgt
    53821 ttcttgttaa tagggattga gaatgcaacc ttgcaaccag attcaagaaa tgagtaatct
    53881 ttaatagtag aaagaaataa acccaatcct ctaccatctt gactgatatc aactgcatcc
    53941 tctttaggtg agaacatttt ccattctttc acatacgata gttccaattc aaaagtaaaa
    54001 tcttcacatc tagatgcaat aaagtttact aactgtccga tacctagtgg agactcaata
    54061 tcattttgta aatattcatt accaagattt ggtagactgc tgagtaattc tacgtatcct
    54121 aataatgcgt tacccgctcc aaagaatgga acgattaaaa cagatttgac attttccaat
    54181 aaatggtttt gaacaattgc atcaatgttt tgtggtttaa attttgtgat tggattaaaa
    54241 aaagctcttt ctgtgtagaa ttgcttttta taggcatctc ttaagaaata tgaatttttc
    54301 gtatcggcta atgcatttaa cagaggagta tccgctgcat aataaaaacc aactctaact
    54361 tttgtaataa aatatccaaa ttgattgaga gatgtttcca gcataacctg gaatttttga
    54421 atcgtttgtg taatcattga tgtctttcca tacaaagaaa gaacggaagt tagatctaat
    54481 tgtggagcat agtgtagatt gagtaattta atatttttga tggttgctct agctgctgat
    54541 ctttctagag gttctataat aacttcgatt acatggtata aatcatttct atatggattc
    54601 tcttgtttga ttcttacttc gattttgttt tccttcatga tcactactag aaggtctggc
    54661 ttgcccattt cttcgccatc cattggttct agagtgatat tgactgaatt gtttaagtct
    54721 ttttcagatg ttactttagt gattacatat cttttgtctt cgtgaactga atttactgct
    54781 ttgctgataa gtctgcttag gatagtagat aatgtctttt gctcttttaa aatatatttc
    54841 ttcataattt gaacaatcct tgtaattaaa tcgaattttc tgttttaaac ctgtttcgat
    54901 aaagaaagtg aaagagattc taatacattc cttgaaaacg caagcatttt ttctattttt
    54961 ggaaagttta ttgtactagg acagatcata aactcaaagt aacccttctc tttcagaatt
    55021 atgcacccaa ccaatccctt tttctgcgat tagattcata agagccttag agccttctgc
    55081 tctcatgaaa taagcttcct cgtcattaat cggttgaata aataaaaaat tgatcggttg
    55141 attggattca cttgtgagtc cagtcatatc aggtgttttt gctacttcga tgtcggaagt
    55201 cctcactgga gatagatctt ttaataagat tgcagatgta aaatttaaat gcaatgcatc
    55261 gggatctctt ctattcatag aaatggtatg tcctgctcct agccatttat tcatgttcca
    55321 tggaaatttg actaattctc ctaaaaaatg aggaacccaa ctttctgact tatcacctgg
    55381 caatactcga actgcgatga taagttcaat tcttgaaaat ttagtataat ctttatggaa
    55441 tagttccact gttggcatgt tttgaccact tagaccaact gtagaataaa tgacaacctg
    55501 cggatattcc ggtgattcaa attttgcaat accgacatag ggaaattttc cgccatctgc
    55561 agaccaatat tttgtgtgag atccaaactt actttctaag aaactaagcc gagaactttg
    55621 aatttgtttc caagaatctt tattggatct aaactcccaa aagttccgag atcgttccac
    55681 tgtatcaaca attactccgt gactagaatc acccaatgga taggctgtga ttgtttctac
    55741 tttggcaaat ttggaataac cacttacacc tttgattcca taccaagaag gcataaaagc
    55801 atgcagttct tcttttataa atacagcgat tccatcccct tcttcagtcc agataaaatg
    55861 cacatcttcc gctttaattt cagaaatgtc tgaatcgtct gtcacctcgg aagataataa
    55921 aatgggagcc aacccgttgg aaaaatcaga ttcctctctt ttatttggag cgggaatacg
    55981 atttcggatc caaagggctc ttatcttcca ttctggattt tgctcagact gaaggtataa
    56041 ataaactgtt ctcccatcat cctctaagaa ggcagtcaag gctccatatg gatttgtttc
    56101 ttggtaaatg attttgaatg aatttgtatt ttccaaactt atttgtgaac ttcagactgt
    56161 ttaaattcag ctagtaactt gtagatattc tcttccatat tctcttctat caggatttca
    56221 ggaaaatagg cagaagcatt gagcaatcct gttaaagaat tttgtcccca acgcttgtag
    56281 ttgcgaatgg attcgatcac attgtccgga ataggtgtat tttcttttct acatttaata
    56341 aaagtatcga ttgcgctttc aggtgatatt aagtatttca aggtctcctc ctaagttttt
    56401 caaaaatatg aatttaagtt aaattaattt ataccatatt gggtttttta gaatgagcgt
    56461 atagtagaat atcgttcttt ttttttaaat tttttaaata catcttacaa tttatttttg
    56521 caaataaatt tgttgttagg atagcaaaaa ggcatcccta gattcaatct atttttattt
    56581 ttgttttaag attaggtttt gaagctaata taattttaat cagtcttata atttttttat
    56641 cctgtgaccc tgtctcagta tacatatttt ctaaaagtgc aattgctttc tcatgtaaat
    56701 tttgtttata gaaaatcatg gcttcaaatt ttgttgcttc aatcgttata gaatcatagc
    56761 caagctttga ctctcttaaa aaattaaggg ctttcacgaa atcgttatta tgataggaag
    56821 cgattccttt taaataagaa attgctctag gtgaaaattc aattttagat tcacctaaat
    56881 tttcgtttat atttagagtg ttttctaaag cttctaggtt tcctgataaa attgagttta
    56941 tgaataccat ccaaacatag gttttgttta agtcactgtt taatttagtt gagtctagta
    57001 aggaaagttc tcctaagagc atcgttggtt tttttcgatt tactatcact tcgtagagat
    57061 aaataaatag tttatttgat tcagattcat tgaatttgcc attgagtgat tgggcgcgaa
    57121 gagcggatct atacattgct tctagatctt cttctgcatg gaaatattcg agataggact
    57181 tgcgtctttc tagagacgtt agatcaatta cctcagcaga atcaaaattg attctatcta
    57241 aaatttgata gtaataggat tttgcaatta aataacttgc actagaatct attggagctg
    57301 tatcaaagta atttaatgtt gcggctttgt attcttggat taagtttttt ggaaaggttt
    57361 ttttatttat accttcattt attttttctt ctaatttcgt aagtttctga acactatttc
    57421 ctgaaaaata cgaaatgatt cttttccact taaaagcaat tccacctata aaagtaatgc
    57481 ctaaaattag aaaaataatc aatgggtagt tgatggattt ctttttgtct tgtttgtaat
    57541 gtcttggtaa atacatgctg ctgtttaaat tagatactta aatgaacttt gatggcttta
    57601 gttgttttaa aataaaagat tccctatttt aatagggaat cttttactca ttgttcaata
    57661 tacgacttcg aagaggaagc tatactgaat tcaattcttt attttcctac gatgaacaca
    57721 acgttagttg tagcagatcc accaatgtta agcatgagcc cgtttttagc tcccttgact
    57781 tggtaaccac ctgctgttcc tgtaacttgc ttgtagaggt cgagcatcat tctaactcca
    57841 cttgctccta caggatgacc aactccaatg agtccaccgc ttggattaat tggtttcttt
    57901 ccattaaagt caattacacc tttttgaatc gcttcatgtt cttttcctgg agcagaaatt
    57961 ccaaaagcag aaacagctgc atattcacta gatgtaaaac aatcatgtgt ttcaaaaaca
    58021 tcaatgttct taacagacat ttttgctctt ttgtatgcat catctacagt tgcttttgtc
    58081 caagggagaa taaatttatt tcctttagac tcttctactt tacctgtaaa tgtaatcgga
    58141 gcaactctat gtccccatcc tttgatttgt ggaatgttag agagttttaa ttttctcttc
    58201 tttgcatatt cagttgcata ttttttagag gctaaaacta ctaacgcacc accatcggta
    58261 acttgagaac agtcagtaat acagagtctt cctcctactg acatattgtt ttcgccacct
    58321 cgagcacttg cgtgttcttt attcataaac cagcttctgg tttgtgcgtt tggatttctc
    58381 tttgcatttg catagttaat tctggaaatt tcagctaacg catccatgaa agtctcttct
    58441 ttgagtttgt atctttctaa aataacatca gctaattttc caaagagttt agggaaaggg
    58501 aactcgattc cttttgcttc tctttcataa aaagcagcag ttcctaaaaa tgctccaccg
    58561 acagcagagt taactgtctt catgatttca acgccaacta cgattgctac atcataatct
    58621 tccgcacgaa tctttgttgc tgcagcatcc aatgcaatag atccagaagc gcaagcagct
    58681 tcgaatcttc cgccaggaac tccattaaat gcaggattta cttctgttaa gaatgcacct
    58741 aaatggcctt ggtttgcgta ttgctctgca tcgaagtttc caacaaatac tgcaacacga
    58801 tttgctttgt ttaatttttg aatttcactg tagtcgattc cagcagctaa tagaccatca
    58861 tctgtaactt ctttcatgat ggatagaaat gtcttacctt cttttgacca atttctttga
    58921 aagtcggttt gtgctccgcc gagtatgtaa acttgatttc cttttgccat ttgaattctc
    58981 cttaagctct gaaaagcgct cttgcatcta atttattttg taatttataa aattgttctt
    59041 ttggattagc ttttgctaat acttgtggta tttcaatttt agccgatgat aagaattttt
    59101 tcgtttcaac tactccaccc aatagatcaa caaaggcaga cgctggaacc caattaaatc
    59161 cgaatcccat agcagcatct acgttttctt ttgtatcaac aacttctcca acgagagaga
    59221 gtgaataact gatgtaacgc gctaagaaat aacggcataa atctgcttct aaaccttttg
    59281 cagttttaat tacttccatt gctcctttat aatcagatgc agcaattttc gcacttgcct
    59341 cttttgcgaa tgcaatctca atcttcggat atggatcata attaccagta gcaatattat
    59401 aaacaaattt ttctctttta ccatctgcag acttagttct tttgaaaaga ccttgtcctg
    59461 atttcattcc tagatcacct ttatttatta gcttttgaat ataatctggt aatttgaatg
    59521 tagcatgagc agaatcttct gtgaattcgt aaaggttatc tactatcgct ttgtggacat
    59581 caagaccaac aaagtcgaca gtcgcaagag gacccattgc tcttccagta aatccaccca
    59641 tgattgcatc cataagtgca ataccacctt tgtcttgata cttttgtgca tactgagcga
    59701 tttcattcat taattgaaat ccaactctat ttccagcaaa ggctggagta tcatttgtat
    59761 aaacaacggc acggcgaagt ttctcatcta gatattttcc taattctgcc tttacttttg
    59821 gatcagatcc tttatgtgaa actaattcac acaaaatcat tttataaggc gggttgaaaa
    59881 agtgagttcc aaagtaatgt ttttgtccat ctgcatcaaa agatttagat agtctttcga
    59941 ttgatagccc agacgaaact gttgaaacga tagttcctgg tttgcgatat tttgcaatct
    60001 gtgcattgat aggttctttg actgaatagc tttctgcgac taattcaaaa acccaatcgg
    60061 actcagcaac agccttagct aaatctttat cgtaagttcc gggaatcatt cttgatttga
    60121 ttgtatcagt tcttacggat ttgattgcct tttcaattcc ttcttttgct ttttccacat
    60181 ctcttgctag catgtgaacc tttgcttcac caaacgcggc aacaatagct gcactacctg
    60241 cacccataga tccgtttgcc ccaacaattg taacagtttt tatctctctc ataaattctc
    60301 tttattccca aaaataattt aaacttagta tgcagaatcc gctaaaaaat ttatgcaatg
    60361 actctgtaat tacttggtaa aaatagaatc atttactgaa aagatttttt tcttcggatt
    60421 ttagttaaaa agagatagat cactcttggt aattaagaat gtcccttaag aaatcatcta
    60481 aaataattat acttctaaat tgaaagaact gatttatttg gtgtttttta tgaatataac
    60541 gcaaaaaata gaagataggg agaatgattg ggtcattcaa cttactcttt cctctttaat
    60601 tctgcttccg attgtgtctt ggtctatcca gaaagtaata aaaaggagat taaatgcatc
    60661 gagtctctgt ctttttaata aaggattgat taagcatttg aaaatgattt attagcaatt
    60721 tctaatattc tcaaagtctt catatatact gacagtttac caattagcat agtttatggt
    60781 aggtttttga tctcatactt tgctaaattg gatatgtttt aattcttttt ggtttcttaa
    60841 aacttattgt ttttcaaacg gtaattctag atcgaaattt actaatactc tacctgcatt
    60901 gtctagagag ttttttagat ttacaacacg aatctttcct tcgtggtttc gaataatttt
    60961 ttcgacaaag caaagaccta atccaaaatc tagagtagga attgcatcat atactaaccg
    61021 agatattcta aaaaatggct caaagattat gttttgatat tcagatggta ttccattttg
    61081 ttcttttgtg tcttttgctg gagtatttag aaccgatatg tgaaattctt ctctaaccac
    61141 ttcaaataaa atatagattt tagaactggc ttcggaaaat ttaagagcat taaataaaag
    61201 ttcttgtata gccttttgga aatactctgg atttattcta attttccgat tattttttga
    61261 aaatgtatcg ctcgctagct taattatatt atttcttaag ttttgatagt cggcgaggtc
    61321 agaaacagtt gattctatca gtttataaat ttcagaaata gttagtgatt ctttaggtaa
    61381 ttcgttgttg atgatataat ctatatcgcc aatcataaaa attagcttat ttgaaaattt
    61441 tgtattttca aatagcattt ctaataaatc tttttcaact aaataataac ttccttctaa
    61501 tttagaagaa gcttgaatca tttgaactaa aggagctaac gttccaagac ctgctccttg
    61561 tatcaagcta ctattgatac tagtcattaa tccactatct gttctatctg aatctttttt
    61621 aattaaattt tctttgaata agttccaatt gagttgatgc tcaattctaa tttctctttc
    61681 cttctgaatg ttttcgttta aaattcgtaa ctcagcaatc tcaaaggctt tatcaactcg
    61741 gttcatcatt tctgttgggt tgaatggttt tacaagataa tcgtaggcgc ctagtttaaa
    61801 gagttcgact gcgatattta catctgtctg agccgttagg ataattacca caggtttgta
    61861 tccagcatca aaaatttgcg ccattaaatc ttttccagaa atttccggca tatttaagtc
    61921 gaggattacg attaacgcag gatcttcttt gaatagtttt agggcttctt tcgagctggt
    61981 tgtagagatg atttcatatt tttttgaaag agttcctttt agaatttcaa ttataatttc
    62041 atcatcgtcg actgctagta tcttgttcgt catcatattt taaaaaagtc tatttgttta
    62101 tttaaagact ttgcttgttc ggatattttt tgactaatca aagcaatgtc ttcagcttct
    62161 tttgcaacat cttgtgtttg atgattgatt tctctcacgg ttgcattgat ttgcttattg
    62221 gtaattgttt gttcctttgt tgaatcagat atatcaaatg ccattttcat tactttttct
    62281 atatgggaaa gaactttttg gtttgaatcg aactggaagg tagttgattt tgaaatccct
    62341 tctataatga ttccattctt tttgatattc tctacgatag tttctagggc ttttgctgtt
    62401 ttatccacat attccttacc tttcgatact tcttctaatc cttcggcaat ataaactttt
    62461 atacttctcg cactttgggc agttttatca gctagctttc caatttcttc tgcaacaaca
    62521 gaaaatcctt ttccatactc gccagctctt gccgcttcga tagctgcatt taaagcgaga
    62581 agattaacct gtccagatat atccccaatc aaaccaatta tctcagcaat ttttttagtg
    62641 cttgaatcga tttgattcat accgccaatc gcactttgca ttaaatcgtt tcctcttttt
    62701 gcttctacag ttgttttagt tgctagttgg gtagcagata ccgcatactc cttcacttcc
    62761 tccgccttcc attttaattc ttccattgca tggaaagttt ctttggaaga ttctgattga
    62821 gataaagcat tttcggaaac tttctgaact gattttgata attgttctag cgaaacgtta
    62881 gactcttcaa ttgaaacaga ctgattcttt gcactgattg ctagttttga tgtccctgtt
    62941 gaaagtgtta aagaattatt ttctaattct ttagcagaaa ttttgattaa cgatactgta
    63001 tcacttaaaa tattaattaa tttatttaaa tatcttagaa tttctccgaa ttcatccgga
    63061 acatagtctt ttggtttttc tgttaaatct ccatttgata ttttattgaa aatgtgaatt
    63121 gtatttaaga taggttttga aaatttactt gttatgattt gaataataaa cgaaatactt
    63181 attgcgataa agaaaataaa gagagacatc tgaatcaata gagtgttagt ctctgagaag
    63241 aaatcatctt tattttggag catgactaat ttactttcat tgattggaat gttataggta
    63301 aaggctattt tttgtttacc gttatagtga atctgtgtgt atcgttctcg attttgggat
    63361 tctgttttta aaagattttc taagcctgaa aaattttcat cttctgatag tatatctttg
    63421 ccaattactt tgtttatatt ttcggtttta ttatcatata aaattgtatt gtctttagaa
    63481 aataggatat actcatattt ggaatcattt tcgtttaatg tttcgcccaa tttacttaga
    63541 gtaaatgcca taccaagtaa tccttttatt tgcttatcaa atagaattgg aattacgatc
    63601 accattatat tttttccgtc taccttgctt tttacaggtt ttgaaaaagt aagtttaccg
    63661 ctttgaataa attctttaaa ataaattcta tctttaatat caaaagatac tttgtcacta
    63721 gaaaaggata ttccttccat atcggacatg taatacaatt ggacaaaatc ttctttgatc
    63781 ccatgaagac tagacatata ttgtttagcg gctaacgaat tagtatctct taaaacttca
    63841 tttttcgccg aagattccat cttagttatc attttttcta aaataaaatt taatctttcg
    63901 ctcgtagatt ttatttttaa gttcatgaat tctttttttg tttcaaatgc attggtaatt
    63961 tgtaatttat acaatccaat gtttgtaagt aataaaacag aaattattat acttacgata
    64021 ggaattctaa tcctgttaaa aatgctaatt ggtttataag gagcataacg gataattgga
    64081 tataagtcaa ttttactaaa atagaaaaat aaattacacg ttattgtgat taatccaaga
    64141 cctgttgtga aaaaataaaa aaaatcggca aatgaaaata aaatcccaac ataataagca
    64201 atcgattcag taagtaagat agtgaataga ttaaacgatc ccattaataa ggtcatttta
    64261 attggatagg cgtttgcaaa acttatggaa gaattggtat tgattccatg gcttgattta
    64321 atttgttcta aatgaaattc tagttttctg ttatatataa caaacaaaga aatagaacca
    64381 atcgctatta tagattgaac aataataaat tccatggaaa agaaatagga aatcgctttt
    64441 tctaaaccat ggttaatagc agctaagaga aaaaaaacga ttggagttat ggaatacagc
    64501 aggattgcgt tgatgagtag ctttcttggg attttgctta tatcagttgg ttgcataaaa
    64561 agaaattaaa ctttaaagat tcgaaaaatt cagtacatat tttgaaaaac ggaaacgatt
    64621 gtctaattaa atttttgagc tatcaattat tcttaattga ctctattttt tatctatact
    64681 agaacaagta ttttgtagga gaaactatga tgaaaattta caaaatattt atgttaccta
    64741 gtctagcact attaatgtct atgtttgggc ttcaagcttg gacagatcat acactaggaa
    64801 catatcccgc actcgcaagt atgcctgaag taaaaaatgc taagcctgtt caggtggaag
    64861 cattcgaaga ttttttaacg aaagaaagtc aaggacttgc tcaattaatt aaagaacaag
    64921 aagaattcgc aagagcaaat attcaaaatt atccccctaa accagattca atagtatttg
    64981 atccaaatga taaaaaaaat attcgaaaga attttttaat ggcgatgaga ttaaatcctg
    65041 aagttcgcct agcttacttt atccaagaat taccaggaac aaatgtttca gataaatttc
    65101 ccccagagaa cgttagtgta tataaaaaca ttgaatttct taaaagactg aattttaaaa
    65161 atcttaaagt aaaatcaact gtttccgctt tagcagtatt agctacagca gcagatgaac
    65221 ctgattatgg tcatgatatt ggattgtatg aagacaatga agtggaacca ggaaaaggaa
    65281 tcatgaccga gcatgggaaa atatatggtt ttggtaaaca gccatttggc gataaaagat
    65341 ttgaatatgg gtcacaagca ccttttcata tgggctttta tcatgaagct ggtataattt
    65401 ataagtttgg tggttttata aaaagaacac tgccagagta tagactacat caatttttga
    65461 cattggcaag atttgcattc aaaacaggac atccttattg gggatatcgt tttactggat
    65521 ggggacttca tttcgttcaa gatttaaccc agccttatca ctcaacgtta gttccaggcg
    65581 taggtcctag taagcttttc tttgcaaatg ctatttcgat gattggtttg agtggagcaa
    65641 aaaatggact ggcaaaaaga gttgctgata gacataccgc cattgagcat tatcaatttg
    65701 agtggtttga aagtattttt agaaataaag agactgacaa taagatgttg atcgcatttt
    65761 cagatatgag tcaagatagt tcatacggag aatttgatca tgattatatt cgtaatgttc
    65821 taaccaaaga gtcaaatgaa agggcaagtg aattagatag taggattgaa aattgggaag
    65881 caatattgaa gtttatggat cacaatataa aatatgaaga tgtcaaagat gattcccctg
    65941 caaaacagga aattcaagaa tttctaatca tactcatgaa gtctttcgga tcacatacta
    66001 gaaattattt aagggctgca cttaagtatt agtcaaattc aaaccttaca caaaagttta
    66061 cactcctgtg taaggtaata ctattttatt ccagttaaga aatacatatc tacttctgat
    66121 ttgtttttgg gagtgaattt tcctctatat tcaaaagtaa aaatatcttt ggtgagttcg
    66181 tagacttctt tagatacatt gattttattt ggctcacaca tggattccat tcgactggct
    66241 gtattgacag tatcgcccca aacatcatag gcaagtttct tctctcctat aactccagct
    66301 actataggtc cgacatgaat tcctattttg atgtcccaat attctataga tgatttttcc
    66361 ttctccgatt ttctttcttt tatgaaattt aaaaaatcga tagccgcaag acaagcatca
    66421 actgcatgtg tttcatttgg ataaggtgcg cctcccacac agagataggc atccccaatc
    66481 gtttttattt tttctaaatt gtatttttct gttatcaggt caaaattgct gaaacaataa
    66541 tctagttctt ggatcaaaac ggaaggagca agtttttctg aaatacgagt aaaaccagta
    66601 aagtcagtaa atagaactga cgcttgtttg ataaagactg gctctgattt acctttctgc
    66661 tttagttctt ctgcaatatt cttagggaat atactgagca ataattcatc acttctcttt
    66721 agtgcatttg caagtaggct ttgataataa ttatatccta aaccagtgta gaattcactc
    66781 tgtttataaa aaataatcca aagatctttg taatcttctt tgcattcatc cacaagcatt
    66841 tctctaacgt tttctttcat gctgttttca acactaaaga ataaaccaaa gtcaaaatcc
    66901 cttccgtagg attttccaac acctaatact ctttcaactt tagctttgag ggaagcaact
    66961 gactcaacgg aattatctct tatatctagg attctagaaa aatacaattg gttatacatt
    67021 ttaaatgtag tctcattgta aggtttaaca ccttcatctg atctagattc atgccatttt
    67081 ttgaagaaat tataatcagg atccgcttga tctttagttt gcggaaactc tttataaaca
    67141 tcttctatca tttgatttgt taaatcttta aaactgattc cttctaatgt aatgaaattt
    67201 aataaagtat ctgatttttc tagttctgat ttcaaatgat atacaaaatg cttcactagt
    67261 atatctgcta acttttgtct agctctcata atttgaatgt tcattgtttc cgccgtaaca
    67321 gtaaaatgaa actaaaaaat gaatctattc agtaaaatta atctcttaaa ctagattcat
    67381 gaaaaaacag tcatgctttg aactaatcaa gtggttgttt tttaataaaa tccaaaaagc
    67441 aatggaagtg aataagacca tatgaatcta aaatcataaa aatgaatcag tttctagaac
    67501 agaaaacttg acgtgatctt gaaaaaggaa aactatgcaa tcactatgtt gagaaaaatt
    67561 ttattgattt tgattctatt ttgttttttg aatgctgatt tcccaaattc gaaaaaccgt
    67621 tatgttctta tccaaactgg aactttgaac ttgagagaaa aaccaaaaga tggaaaagtg
    67681 attggaagtt tgcaagcagg agaaattgtt acaattttgg aagatgcgaa agacgatatt
    67741 acttggaaaa aaattaaaac taaatctggt ctagtgggat ttgcttctaa tgaatttctt
    67801 ggatattttc ctccggaaga aattcaaaaa gcaaaactca ttggggtttt aggaggagcc
    67861 ggggataaaa caaataaaat gggtatacgt gtgttagccg cacatttttc cggacaatgg
    67921 tatggctttg atgattccgc tgattttgtt tatttactta acttaattgc aaaagggaaa
    67981 aagagtttag atatacaata tgctgcaaaa aaagtaaact catttcaaac agatacactt
    68041 ggaaaatatg gatgccagga atttagggga ttagaagggc gcgtaaagac tcccgtatct
    68101 gacacttttg aaaagagcta tattgctacc tataattttt ctgattcgaa actagattat
    68161 acaatcaagg aagatgtatc agcagatatt acatccaatg tattaaaaga agcaatttct
    68221 gtttttaaga aaaataaagt aactaccaaa gagctagaga atatcactga aaagaaaata
    68281 aaaatgctct ctagtggaag cggcaaatca tacttagtcg ctcgttatgc tattaaaaac
    68341 ggtttcgcgg aaagacacta tttatccttg gttgcagaat taggtaagaa taattctctt
    68401 aaatttattc atagcaaatt tgaaacttta gaagaagaaa gaggaaacta tggaggcagt
    68461 tatcattttt ctggaatcgt tgatattgat gatacaggag ttcctgctct gttatttttt
    68521 catattggat tcgattctgg aatttatgaa ttattcaaaa tcggaaacga caaaatcgaa
    68581 tctgtattta tgggaggtgg ggacgcatgt tgatcatgcc atcatttcaa agaacggatg
    68641 gtctttaatc atatcgtgat agtcctctcc aaacgcttct tttaattctg gactgaagtt
    68701 ataggattca taaatcactt gctctatttt caagtcatag tcaggaattg taaaattcaa
    68761 cattctagat gcgagtatat gagcaagtcg agttaatttc acgacgtcag tcatcatgtc
    68821 aatagcattt ggttcgaagt tctggtgtcc aactgattgt tgtaggctga ctggcatatt
    68881 ccaaagttta aatacctttg cacctgcttc aatatgatcc attttaaaat tttctctttc
    68941 cacatgtaat aaatctctta ctccagtaat agctagttct aataactccg agtatctggt
    69001 tggaaaattt aaacctaaaa ttgtcatacc aattttttgt aaaagtcctg ctaagaatac
    69061 ttcttcgcgg atacgtttta gaccaagagg atttgttaag tcaaaaccga tgagagcagt
    69121 aagaacaggc aattcttgta atactttttt atagattggg ctatctagat ttttagtaaa
    69181 ttgcttattc gactttaaca ttactaaatt cttaactgtt ttcattccaa ggagtgtaat
    69241 tgcatctcta agagtatgaa ctttacctga tctaccataa aaagctgagt tggcaatttt
    69301 cattatatcg gtagtgattc ctttatcagg gcttatgatt ttttctaatt cttcactccc
    69361 gccagcaggg ttttctgtgt caaaactcat cacttttata gctacagtcg gaaggggagg
    69421 tattgataaa tcggaaagat gtattgtttt agaggtggcg tctgtcgcat ttataattcc
    69481 tgctctacca agcacttgtc ccattttatc tatgattgca tttcgattgt aaggtttctt
    69541 taaaaaaccg tggattttta attggagtaa agattgaatc atactcttgt cctcggttcc
    69601 tgatataact aaaatcttca ctgttggata actttgatta atctctttga ttacttccaa
    69661 gccgttcttt ttgggcatat gtaaatccac acagatcaag tcaggctttt tgcttgtgtg
    69721 ctttaagtgg tagagcaggt catcaccttc tgtagattca tgtaagatat caaaattttc
    69781 agattgaaga aaacgtttta acaaacttct atcaatcatg ctatcatcta ctagcataac
    69841 agtgtatggt ttaagcgtgt ctggatttgt tcctactgag atatgagttt ctgttggcat
    69901 ttttttttcc aataaaagat ttggcgccct ttatatgcga attacaaaag ttttcaatca
    69961 aaaattagaa agtaaattat ttattttcaa taaagtttgt agagtttagt agagatttag
    70021 ataagagctc acctaacaga aatatgtcag gtgaggtgaa gaatggtttt attttctaga
    70081 tttgtctgtc aatgcgtcaa aaaaagttac aatagatttt acttctgcat cagttaattc
    70141 tttgtttaat tgatgataac ccattttctt tacagcatct tcaattgtag caatgcttcc
    70201 atcatggaag tatggacctg ttaacagaat atttcgtaag gaaggaactt tgaagaaata
    70261 tttatcagct tcattctttg tgacttcata tcttcctaag tcttctgttt tgtatggatt
    70321 tctttctcct agtttgcgat agctatttcc cccgagagtt gctccataat ggcaaccagc
    70381 acaaccaact tcgataaatg ttgctaatcc tttttcttca gctgcgttta atgcttttaa
    70441 atcacctttt aaataatcat caaaacgatc agaagtctta agggttcttt caaaggcagc
    70501 gattgctttt gctaaattgt cataagtaag tttttcttta tcagctggaa atgcttttgc
    70561 aaaaagatca ggatactcag ttttagaaag tttttcaatt acagcttttt ctgaaggcat
    70621 agccatttca at
//
LOCUS       DAOQOS010000012        64940 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_156_length_64940_cov_7.121292, whole genome shotgun sequence.
ACCESSION   DAOQOS010000012 DAOQOS010000000
VERSION     DAOQOS010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 64940)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 64940)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64940
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_156_length_64940_cov_7.121292"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            196..1188
                     /gene="rsgA"
                     /locus_tag="PLF66_04170"
     CDS             196..1188
                     /gene="rsgA"
                     /locus_tag="PLF66_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020769307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HMZ63268.1"
                     /translation="MNLEKTSSTVKENFIVSKIYGAYYEIYSPKLGFKRAILKGKLRL
                     NKSEERHPFVVGDRIVAENESPERGDWIIESREERVSFLTRQSSSSDKHILCANVDYV
                     AIIASLSGPETKDGFIDRSITACYHSNVKPLIIFNKKDLVTEEVLSERVQKYKDLGYQ
                     IFPITCTDLESIKDLLATIQDKTTYLVGNSGVGKSSLINLITNSTEQRINSISHSTQK
                     GKHTTTNSSVIIVNPTTILIDSPGIKEWGLLHLSKGDILSSFPEINKYKKKCRVGNCC
                     DAGNNCLMIQKLDKLDENRAKSLESMIESLEIPYRIRVGNLKSGKLRKIKSHWD"
     gene            complement(1190..2467)
                     /locus_tag="PLF66_04175"
     CDS             complement(1190..2467)
                     /locus_tag="PLF66_04175"
                     /inference="COORDINATES: protein motif:HMM:NF026253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HMZ63269.1"
                     /translation="MKFHWGFMDIKGNVKIPPKYEALGSMSDNVALAQMNDKWGIINQ
                     KGEFILKPQFDDVREFEDGLAWVIDQCKEWVVGECERGKWGLVDKTGKILIPPKYDGV
                     MSEESPYINRIFGRMSGELFKEKIFFGKDGLAKVYIDAGDTFNRIRKYGVVNKEGIEL
                     IAPEYHKIGFFQNDVAPFAIHNRKGIYFGLIDIAGKIILEPTYRSIESLVSDEEVLWA
                     ASYYDSDEKRVLIQILDRDGKEVTSERFYQVKMFQNGLAYARKTHTSLWGRINRKGEW
                     VLKPFLKSLTEITPQEERKKTSIEINGKQFFGNDTDKLLWFAETKKDNFDGQVRDYWG
                     FADREGNIIYPANSFEKGNFVDGVSWVSLRYRKKNNGHIIYYALVDESGKELTEPIYM
                     YVLPFRRGLGIAQVFGGHWGYINTKGKVVWWLK"
     gene            complement(2754..4169)
                     /locus_tag="PLF66_04180"
     CDS             complement(2754..4169)
                     /locus_tag="PLF66_04180"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HMZ63270.1"
                     /translation="MIKNLISIYKTIYVNVPFRYRREFADKLRMDNLSRFNLVSFFIL
                     LLSPFMVWTHIEIVSHNYCNLYLYYCEVLFGAHYLGVFFALVYLIFYRFLNPAKWNKV
                     LLEGFILISIFSFLGLFALSSLASQRLTGNISFFTTGAVFIAALFVTFKRYATLVYFS
                     ILFLFILGLYNFQTNPDLFFSNAIHSVVSIFFAYSLNLIFFHYKLDDFLKSKTIDQKT
                     KEINDTLDDLKRDISVAKKIQTSLMKKNFAHIKNLNFSVNYIPLDEVGGDIYDISELS
                     HKCIRIFLADATGHGVQAALITMAIKGEYESFKHTTHSPKKLLKNLNNDFVQKFGAIH
                     SFFSCIVVDIYPEKNKIIYASAGHPDQVIQRKDWSLEIISRTGKIMGIMKDAEFEEKE
                     IRFESGDKLLLFTDGLFEEFNSEKEEFGEKQILSSIKEYSDKDLKELFEEIMKNLNEF
                     LENSSRQDDITFIGVEYDRKE"
     gene            4422..4796
                     /locus_tag="PLF66_04185"
     CDS             4422..4796
                     /locus_tag="PLF66_04185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63271.1"
                     /translation="MNLNENIEIKIDFSSLEEIYGENKKDFSLILQKIQQEYTKYLAT
                     LEEEIRTENLDEFRKTRHKISATLLLLKLEKFHEYLTFLKEHFAHGFHDKEESIAILK
                     DCFAKTQSLIASKLEDLSSQIH"
     gene            4821..6068
                     /locus_tag="PLF66_04190"
     CDS             4821..6068
                     /locus_tag="PLF66_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010575800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HMZ63272.1"
                     /translation="MKLKKILILLFLLQIVFTLSMKIFSYIGDDSQELLTRTLQYFTQ
                     EDIDKGIEYGRRGFFARIASLLINFILTGIFVFSPLAIRLEEYLLHKANNRYYPMIFL
                     FFLTYTAIEFIISLPLQYYFGFVLEHKFGFSNMTFMDWLIYTGKSVLVGLVVGSFLSI
                     AAAFILKTFERTWKYILPIGSLVLGLAFSVLAPILITPIFYDYKPIEEGSLRTKIISL
                     CNHAKITVENVYVINESKYSGHTNAYFTGWGENRKIFLYDTLIKNHTEEEVISVLGHE
                     IGHWKYNHELTFHIGNTIELFFICLLLSYIFKKVKEENEFKIKEIYSPSTWPILFLVV
                     SILGTITSPFWNTFSRYQESQADMEALVLTNDKKSFIDTEIKMARDNKSRLNPHPFTV
                     WYSYSHPMTIDRIKMAEDYQPQK"
     gene            6072..7502
                     /locus_tag="PLF66_04195"
     CDS             6072..7502
                     /locus_tag="PLF66_04195"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HMZ63273.1"
                     /translation="MSNIEFLENLNVELNDHTLREMIFKRLMKEYGRVIQRELKKNPK
                     TLSVRMRDGHTYEEFLTQQLEDVIRIIGEFTHGPSFFEKIYVEHLDDGEFENVTAKNL
                     ILTYRYRIESFLYSTNFEMDKKLRTAIEHVVSRLVETPDYIGNFNLQEKHIRTEMIAA
                     VLKDGDDEFKRKASALIEKQSLIVMNTMNEIFVERLTTTLLEEKELKHELIARQKAMD
                     EELKRARKIQRNLLPKSFPSNVGLKFYASYIPMESVGGDFYDIQVFPNPDEIPLDRIG
                     VLIADVSGHGVPAAFIASMAKISWQNNFEQFKTPSAILERLNLQMVENTAGNFLTAFL
                     GVFETKVRHGNLEMETPDTNKRGVFHYASAGHFSPYIIRKDMDSITLKVKGQMLGVFQ
                     NIRIEEYSIDYYQGDRFIFYTDGLLEMKNYNGEILGEERLFQIFSETKNLKGNESCSY
                     ILEKLWNFSEGKTVEDDITLLIIDAD"
     gene            7512..8528
                     /locus_tag="PLF66_04200"
     CDS             7512..8528
                     /locus_tag="PLF66_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020779603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HMZ63274.1"
                     /translation="MKKRRLGKSGMVVSEIGMGTMTFGSSCDEPTSFKILDKAYDSGI
                     DFYDTAEIYPVPPEDKWVHRTEEIVGKWLKTKPRDSVLIATKVCGPGHGWFSPPVRNG
                     KTALDRHHIIRAIEGSLRRLGTDYVDLYQTHWPDPDMMYEETMYALSELVASGKVRYV
                     GCSNETPWGLMKSLWASEKNRLIRYESIQNNFSMLNRRFEDALSEVCKKENISLLPYS
                     PLAGGVLSGKYNGGKVPDNARFTRYAKLGDRQKKMSNRFLNDFTLKATDEFLKIAKEA
                     NMSLVTMAVAWSKQHDFVASTLIGANTVEQLTESLKATDVVLSQEILNKINEVSKNIP
                     YPMG"
     gene            8534..9871
                     /locus_tag="PLF66_04205"
     CDS             8534..9871
                     /locus_tag="PLF66_04205"
                     /inference="COORDINATES: protein motif:HMM:NF017743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63275.1"
                     /translation="MQELTESIELSQTKIISLNSQLQGIGDNTNLSFRYTNIGDEVSY
                     EARGRGKNKYAKIHSVIRNKPYNPKCKHFGDCGGCSGQHLDYSTQIQIKTNPILNQFK
                     KEYNLDLELIEADLKYQYRNRMDFAVFPNFIGLRQAENFRRIIDVSHCSLQSDWANEE
                     LEQLRGLIFEEYPNLAFNRKTGEGYLKYLTLRTSVDLTDTITIFTFTDEFQETELESK
                     FAEDILKFSSSNNIIFCFNRKQSEVSAEGKFKILRGKSYYEETFFPKTIQVPFNSFFQ
                     PNPQGFMPIIRFIETKIDAIKRPTLIDLFCGNGFFGILFGEKFSKLVGYDWVESSIQT
                     ARENLNRIYPEKEILYKKKDLIQFKDVFSDSKLEDSVLILDPPRNGIGIKLCEFILNS
                     SVQEIIYVSCNPYSQLEDLKILSQKFKILSGIITDPYPQTPHLESAIFLKRYE"
     gene            9956..11176
                     /locus_tag="PLF66_04210"
     CDS             9956..11176
                     /locus_tag="PLF66_04210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63276.1"
                     /translation="MKTSQSLLTSTRLQKILLWLMIIYSFEACFVKNSTHSSFWDNFF
                     SNQQLLIDRYADTDLYPILFGSLKPEEEKLLTKKDNKLYLSGYVTKQNNSLIPKIDDK
                     ENPTEQDVYFTKVEYIKSEFKERPTYKIKRKTNFRYPTYTMKDFFHWLEVFSESVNDE
                     EQLTILDRSVVYQFLCSEFKCQSELNTGSATLSFALDERMKVKYKTFYNSLSDTLSKL
                     KFKMKVFSKSEKIGEIYSEGKTIFAKILHLKKGQLHNLENLTLYFEIDLNFFGLRIQV
                     QNLEYHIHIKSSETEEILTGKFPIYPKYKVSGRLFYLLPPGLINIFIPQDIESYMADF
                     FDLLIRTGDSEGANFTTRIKKLENNEVNVTFESFSEIFQKPFRPLASNRKQKREGRDF
                     YLDLRNKIVEDLRP"
     gene            11238..13085
                     /locus_tag="PLF66_04215"
     CDS             11238..13085
                     /locus_tag="PLF66_04215"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HMZ63277.1"
                     /translation="MQDIHTTTRLTALESKSIQFSIRYKLILIVSLIIILGLSAIISL
                     VTIFYKSNSEVLIQEYNLSLARLAGIQVESNLKNTNYRAQAFHSSLIQKNISEKEILE
                     KTNHFFTKNPKFIFLGIIQKTENDSFKFENEFMQEKVFSENKIEKSLMPIIHEVVKDG
                     ISAAFKGTTSVINASSKFNFPVLALVVPSETNPDQVILLYLEPTEIMGAFKSALQTDL
                     FDVFMINQKGEVIAHSEDTKTVAQENKAEVPIVKKMLSSNVDNGSQKYFYNGEEYLGS
                     FQLLDFSGLGIVSAVESDKVFEAIYQIQRRNIVITIIILTLSFIVVYLFANTLTVPLT
                     DLVGASKQIEEGIYKVEITPTTRDEVGLLTKSFIRMAKGLQERENIKNTFGKFVNREI
                     AEKALHGELQLGGVKRNCAIFFSDLRNFTGMSEKMQAEEVVVFLNQYFTEMVECIYKT
                     NGIVDKFIGDAIMAHWGALYTDGNDTGNAIDAALMMREALIQFNKDKSHGDRPKFRFG
                     CGVNTGEVVAGQIGSPKKLEYTVIGDAVNLASRIEYMNKEFGTDILISEDSYKEVQGK
                     YKVVAMAPVEIRGKSKPQQMYAVLGKIDDSNAPKDLKELRQIVGIPFKD"
     gene            13088..14734
                     /locus_tag="PLF66_04220"
     CDS             13088..14734
                     /locus_tag="PLF66_04220"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HMZ63278.1"
                     /translation="MRFRLRDYFVFLICSLVLAACSYYLYLDFNKRPEDRGAVKQGYI
                     TFKYKVAQRKFPSRMIWEDVEQMLPIYNKDSIRTDFLSEATVTLNSGIKIELDPDSMI
                     VLNIHEKLVNVELEKGAVQVSTTKNLSNPNEFVLSHKKTKVNFQESTGQFKIQENGSN
                     LEIVSSKGSITLETETKKENIFEKQKANVNLDSNSVEKVDLKNFEMQPPHNVRYFIEE
                     DTKEIIFIWNEKSSQDTEISISQDRSFKKDTFRANVKDNKFTKSFSEGIYYWKIKSQA
                     GEESETRKFRIIKNPPFLLISPSHEKEIKTKDGNSTVHFIWTKRELALSYHFEISNDE
                     SFKTILYSKNVFKNDISVPLTIGKYFWRVRSFDSIQGAKVFSSINSFSIDKEPITEKS
                     TVSSTEEEVEPIEEVKTAKEANIDKPQESKTVETKTAQPTILKNPTLLFPRANSTVDM
                     NKMDSIPFKWQPVAGAKSYNIKLVQESNGELILQTEVKAANYNFTQLDKLDVGRFVWT
                     IEAIPEQDSIDRSSSTAKFNITLGDQPAAPETISKGKKEE"
     gene            complement(15440..16615)
                     /gene="chrA"
                     /locus_tag="PLF66_04225"
     CDS             complement(15440..16615)
                     /gene="chrA"
                     /locus_tag="PLF66_04225"
                     /inference="COORDINATES: protein motif:HMM:TIGR00937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HMZ63279.1"
                     /translation="MNKALEVLFVFLRLGFISFGGPIAHIGYFQDEFVNKKKWLTEKE
                     YVDIVSLCQFIPGPASSQVGMVIGRLRAGFIGSILAWIGFTLPSALLMISFALGILHY
                     PELFPRKILHGFKIVAVVVVAQALLQMGGKFCYEKKRIFIFLVSLLFLSFMQNAIGQI
                     IVILVGGLLGKILLREESRENAKVQWSWYLSSFFTILFFLLILFLIPILENYFPFAFI
                     QLLGGFYKTGALVFGGGHVVLPLLKEEMVANGFVKNEVFLSGYGFAQTLPGPLFSVAA
                     FLGSASSVGLDSVICGLLSVTAIFLPGFLLVYGFFPLWEQIKNLDSFQSIVSGINSSV
                     YAILFFAFYDPIYRTTILSVKDFTISVLLFSLLQFWKLPIWLIVILSPVLVYLMSFG"
     gene            complement(16622..17398)
                     /locus_tag="PLF66_04230"
     CDS             complement(16622..17398)
                     /locus_tag="PLF66_04230"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HMZ63280.1"
                     /translation="MNKKALITGASRGIGKATAIHFAQKGYDLVLVSRNERDLKSVAE
                     ECHAIGVKVDYVVADLSEIEKTHELVSHLQSRFPDIQTLILNAGISTSVTFEDNTIEN
                     IQKELNLNYISPAFIIKSYIPKLKSLGGGNIITVSSFSAIVPFPGNSSYAATKAALYS
                     LCTSLKIELEQYQIHVGCVLPGTTRTEMTKQFHDTPFVPFDDPSEIAQCIEDSIVKRE
                     SVVIPGILYNAAAFTYKMFPKPVDFLMSLAAKVVLPKLKK"
     gene            complement(17446..18378)
                     /locus_tag="PLF66_04235"
     CDS             complement(17446..18378)
                     /locus_tag="PLF66_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004784707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HMZ63281.1"
                     /translation="MKTALAYREEFLDHETGQQHPENYQRLVSIINKIKSTSYYSELI
                     QPEIREATEDEIATIHSSAYIKDFQKRVKQGVGYFDGDTPYSSGSFKAATLASGSGIA
                     LADVILRGEADNGIALVRPPGHHAEKYHAMGFCMFNNIAITAKYLQSKGIQKIFILDW
                     DVHHGNGTENSFYADDTVFFASTHQYPFYPGSGAETDIGTGKGKGFNLNIPLSRGSSN
                     EDYLNAFTEKIVPAIDRFQPDFILVSAGFDAHKDDPLGGMNLTSSVYERFTDIIRKKA
                     EEHCKGKLISFLEGGYNLSALADSVEAHIAALKS"
     gene            complement(18378..18680)
                     /locus_tag="PLF66_04240"
     CDS             complement(18378..18680)
                     /locus_tag="PLF66_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016546280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63282.1"
                     /translation="MIYSILITLLFILFLHLSKKSLGFSYTYFSITSALRNSILDWKK
                     VSSIQLLSAYFLFMLLPFFWGLTFYLKSDANVVVVILGFTWIYNWTKYIFLNEKSR"
     gene            complement(18757..19737)
                     /locus_tag="PLF66_04245"
     CDS             complement(18757..19737)
                     /locus_tag="PLF66_04245"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016615 - malate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HMZ63283.1"
                     /translation="MSKTVKVAVTGAAGQIGYSLLFRIASGQMFGPDTAVELQLLELE
                     PALPALKGVVMELDDCAFPLLQKVTVTSELDVAFNDVNWALLVGSVPRKAGMERGDLL
                     KINGGIFTKQGKAIEAKANNDVRVLVVGNPCNTNCLIAMNNAKGVPSNRWFALTKLDE
                     NRAKTQLAQKSGQPVSKVTNLAIWGNHSATQYPDFYNAKIDGKPATDLIKDEVWLKGD
                     FISTVQKRGAAIIAARGLSSAASAANAVVDTVVNLTTPTKSGDWFSVAVCSDGSYGVE
                     KGLIFGYPISSDGKDYKIVQGVPLNDFGKEKFKVTHDELVSERNDVKDML"
     gene            complement(19747..20757)
                     /locus_tag="PLF66_04250"
     CDS             complement(19747..20757)
                     /locus_tag="PLF66_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010574887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HMZ63284.1"
                     /translation="MENEKKGLIALSAFTILYVAFSIPAMIDYFKSKSYIEINGKKFT
                     ESDLEKEKPAVIMKLKKDYVEGISRAFEQFASDKILELEAKEQNIKPNEVVSKGVGSY
                     TPSQTEIAAIYEQYKDQFGGAKLEQVQDRIVGYLKNLKEQEYYAELTKKYRIDYHMEK
                     VKQVKQNVAEKGNPSIGPDKAKVTVIEFSDFECPYCKRSQETTRQLREQYKDKIRWVF
                     RDYPLPFHRNAMFAHIAANCAIEQNKYWEYFNLLFDNSDNLVKENVILLAEKAGLDKT
                     KFNACLADSGKISEEIQSDIADGQSVGVNGTPAFFINGIFVEGAQPIGAFQKIIDEEL
                     SK"
     gene            complement(20825..21286)
                     /locus_tag="PLF66_04255"
     CDS             complement(20825..21286)
                     /locus_tag="PLF66_04255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63285.1"
                     /translation="MKFSMIFLVVHFLLIGSILGQVAPKQKRKQILLEYKSPTKTIRI
                     LDTSFVLTEKKDVYDNPVSSIPSKSTFKEKDYTLTKKEVYDLIKFIEDNEFFQLKDAY
                     GAPEAERNYPTSIAVQMHKKEKVVVYRSNPSFESSPEAFKKVEAYILKLRK"
     gene            21563..23842
                     /locus_tag="PLF66_04260"
     CDS             21563..23842
                     /locus_tag="PLF66_04260"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HMZ63286.1"
                     /translation="MKIPMPSNIFWNLSLRLELFTSLIPIPTFVYFIAVVGAFKTEED
                     IKSVAISGLICGTYTVAWGLLYRYFKLRNIIRDISFLKEGKTISDKRKEKIKLNILHY
                     PYQEGRTIIARWLLGATSGLILFGFLSGQWFPTIPTISLYLGLLFVLPISYVIYLFGS
                     ESSLRGLLLLPSIVNIPVPSEKILYMGYFRRILLAILSVAMAPITIFGYIIYAIVAGN
                     LSFKDPILHVTLLSLQCITSLGIVAYVIARSLKDGLAVTNNALLELGNGNFDVSSGRT
                     SADDFGEQAHLLAVIISKLKNLYQEITDLNLNLENKVEERTRELNLSLTEIKKLKIQQ
                     DGDYFLTSLLAQPLFINANKSNEVKTDFIIRQKKEFEFHNKKADLGGDICITGNLKFG
                     TQENYSSFTMAMNGDAMGKSMQGAGGSLIMGVVMNSIMARSASKKKILNSNPVKWLTD
                     VYHELNSVFLAFNGSMLLSATVLLIQDDTGEVFYWNAEHPFTVVYRDKKASFLENGLE
                     LRKLGIESEYEFRVHNYQLLAGDVLILGSDGRDDIIMNINDSREMNYDEKLFLKFVEK
                     ADGKLDQLEKALKESGEITDDLSLLRVGYKENIQYPSSEEFIQLQSEAYDKASNYYKL
                     GNVKEALGILLKAYSPNYYTKKFNRLLGVLSYQNKDYATAVDVLSKYVKEKPDSIDIL
                     YYLSIAFRKLGNYDKSIEIGNQLFKLKQNHINNLINLCDLYRLTAQKEKSIYFLELAN
                     EIDSMNPNVVKLANLIRQS"
     gene            23934..25814
                     /locus_tag="PLF66_04265"
     CDS             23934..25814
                     /locus_tag="PLF66_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016762357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMZ63287.1"
                     /translation="MKIFRRLLTYSFKYKYRLISGLILSLLVSIFNGVSLTSMIPIFD
                     SMGASKNYKFQISITKRDLSAIKIVESGENFSRLDQIEYKLAKLKVRLNAYFNELQPE
                     EIVFFFVILVFPIYLLKLLCLTGAIYFINSTGYMAIRDLRLEIYKKVQYLPLNTFVQE
                     KTGILMSRIINDVDVLAKIISSDLKDAINDFFYVVTHLLLLFFISWKMFLVVFIVIPL
                     IMGPISAFTEKIRKATKNQQERLSALNGLLQEVIAGIRVIRAFSMEKRESGRFYGINK
                     ELADKTFKGHFYHQVGPSIIELFGSVLAAIFLAFGAYLISHENFSKGMFMAFFLTLIF
                     LMRPLKQLSMMYNLIQSGVSAGERVFELVDAKTDIENPPHPLKLGKLQKEIHIKNVSY
                     TYPGSEKSSLKNINIKVKCGETIALVGASGAGKSTLKDLIPRLIDPTEGEVLFDGIDI
                     RKFNFKDVRRKIGIVSQEVFLFNASIRDNIAYGSLNASEEKIKKACDDACATEFINSF
                     EKGLDTVVGEHGVMLSGGQRQRISIARALLLDPEILILDEATSALDAESERLVQAALE
                     SLYKNRTTIIIAHRLSTIQIANTIYYMEDGEVLESGSHNDLIALDGKYKKLYDVQFER
                     VS"
     gene            25819..26997
                     /gene="lpxK"
                     /locus_tag="PLF66_04270"
     CDS             25819..26997
                     /gene="lpxK"
                     /locus_tag="PLF66_04270"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HMZ63288.1"
                     /translation="MRILLYLLYPLSLLYQFLFYLDRLLKKKQEIPDAFTISVGNLTT
                     GGTGKTPLTIYLANLVHSIFPEKEVIVLSRGYRGKKTKEGMLVETYSNPADSGDEPLL
                     IKAKIPFANVIIGRNRFESFQKFALSKKKSFGFSELNPLTDINGRFSPRFRARNRKRF
                     FISQSDDGMEAPVIQGVKRVVLLDDGFQHHAIKRDLDFVLVDANNAFGTGFTIPLGSL
                     RERITAVKRAHYIIFTRYAKENAANVEILKVKFIRINPDLKFYNLTYLPSPLINHKRE
                     IISLESLTTKKVLVFSALGNPFSFESLIESYNPKEIKKVRYPDHFAYDEKTMNQLFQE
                     LKNYDILVCTEKDYIKIHFMRLKNEEVDKIYVLPVDVFINKQSELKSDLQRLISSKND
                     "
     gene            27042..27113
                     /locus_tag="PLF66_04275"
     tRNA            27042..27113
                     /locus_tag="PLF66_04275"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27074..27076,aa:Pro,seq:tgg)
     gene            27121..27194
                     /locus_tag="PLF66_04280"
     tRNA            27121..27194
                     /locus_tag="PLF66_04280"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27155..27157,aa:Arg,seq:tct)
     gene            27215..27287
                     /locus_tag="PLF66_04285"
     tRNA            27215..27287
                     /locus_tag="PLF66_04285"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27248..27250,aa:His,seq:gtg)
     gene            complement(27291..27953)
                     /locus_tag="PLF66_04290"
     CDS             complement(27291..27953)
                     /locus_tag="PLF66_04290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63289.1"
                     /translation="MSLEEAHRLIVERELGIGNLELSEKILLDRIIEDPSARAYGLLA
                     EIQYWKGETALPKDKIAIYEKGIEFGKKGVAIDKDNIESTFWLGVCYGLLGEERGIMS
                     SFFLLDPIEHCFTHSLRLDESYFYGAPLRSIGWFFHKIPSWPISKGDNKKAASYLEKA
                     LIYGPDFYLNHLYIAHVYKALGDHKKYHYHLEWILEAEPTRKFAQENQRHKELAKKLL
                     GR"
     gene            complement(27998..28204)
                     /locus_tag="PLF66_04295"
     CDS             complement(27998..28204)
                     /locus_tag="PLF66_04295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63290.1"
                     /translation="MNFELTPNTGAVFKVQNKPSDRLPDYEGHVNIDGKVLRVVGWKR
                     YGRKSGKQYMSLLFTELTNPLPQT"
     gene            28904..30421
                     /locus_tag="PLF66_04300"
     CDS             28904..30421
                     /locus_tag="PLF66_04300"
                     /inference="COORDINATES: protein motif:HMM:NF012323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWD domain-containing protein"
                     /protein_id="HMZ63291.1"
                     /translation="MKVNNLFLVYLLFSLLLVSCDKKDKKPLLIPFQNISVHGGQTSQ
                     GNPSDGRESVGSNGSQNSESHENESSNSSGGSSKSCSLDNPGHSWGDPHLKTLDGLVY
                     DFQGLGEYWLIRTEDGSGIQVRQEPWKSNKKVTVNTAVAAKLGSNLVSIYLNGKVYVN
                     RTLTTIASGSKIELGGGSVALSGKTFKITLPEGTVFTVALNTSYINVYLTTVRLNGKV
                     SGLLGNCNGNFKDDLTPRDGSNVISPISFNNLYLVFGDSWRLKNGESWFEYGSGESFG
                     SFDGIPEKITTIDPAAIDQAELVCQAQGITNKVVFDACVLDVALTGDPSFAQAIDEAI
                     GSPAAGASSLYYEILAAITDTSSSGNGGTDGSGGSTGGGSGSVDNGGGSGGSGSGGNS
                     CSKEDDDKEHHGKDKDKRGDNGNHYGRDGKENKKGNREESNENRFDNHDGKKNGRDRD
                     DERENNGGKNKCSKDNDDSDDSVSGRCSKNSNASIKNHGRCSIYWIWGQKKKSSD"
     gene            complement(30844..31599)
                     /locus_tag="PLF66_04305"
     CDS             complement(30844..31599)
                     /locus_tag="PLF66_04305"
                     /inference="COORDINATES: protein motif:HMM:NF025736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HMZ63292.1"
                     /translation="MLSNVVLKLKEKPPILRNTIMALVLFLIALPGYLLINAFYSVES
                     PANLKLSIDDQIPFLPWTISIYYWIYILIFFPIFMIRDFELFLAGSKAYGFGIAISFF
                     TFLIYPVVIERPVLTDYKNFFEWWVLLNYTIDKPTALFPSMHVSNAFLSSIVVLPWSK
                     RIGFIANLFSFCICLSTLTVKQHFVLDVVAGFLLGGISYLIFVFPVVRKKLSLPKEKI
                     LMPERLSIYVLLIGLSLTFSLFIVYIFWGKYIL"
     gene            complement(31616..32569)
                     /gene="murB"
                     /locus_tag="PLF66_04310"
     CDS             complement(31616..32569)
                     /gene="murB"
                     /locus_tag="PLF66_04310"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008589606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HMZ63293.1"
                     /translation="MRSLEETQKKEIIECLNKKKIGFRTNVNLSVLSSFKIGGISPLV
                     VEPETKESLLDALLILKKLDLPYKILGGGSNLLISDNPNDFITLRLSGEFKEFEKVEE
                     GKFYIGAAANTTPTFKKISLLGFTGVEFLSTIPGWVGGAVIQNAGCYGGELFNKVEFV
                     DFLKDGVIQRKTQKEVEYGYRFTEFLKNKDSIILGIQIQVEGGNLEEIEASLKDKRDR
                     RNSSQPENKKSAGSVFKNPNLKDENGNPIKSWQLIDRAGLRGLVKGGAQISPEHCNFI
                     VNIGGATAADVSYLVNTIQEKVFGTSGIKLEREIEYFGTIS"
     gene            complement(32882..33514)
                     /locus_tag="PLF66_04315"
     CDS             complement(32882..33514)
                     /locus_tag="PLF66_04315"
                     /inference="COORDINATES: protein motif:HMM:NF025084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RloB family protein"
                     /protein_id="HMZ63294.1"
                     /translation="MNNKILILVEGENTEVHYFQSFKKIEKYRRNLVGLDIEIYKPKD
                     HSPKGLLKEAKKKIDDLKRIKLKYEKIWIVLDRDGHFGVSETFEETRVWNQNKKNTKI
                     EIAFSNICFERWILLHFEKSARPFMSCDEIVSYIHANHDSEYNKKIHYDFTELIEETK
                     LKVAIENAKWLERQKEDDLENMQLYDINPYTTVHYLAEYIFAVATRKSTL"
     gene            complement(33532..34782)
                     /locus_tag="PLF66_04320"
     CDS             complement(33532..34782)
                     /locus_tag="PLF66_04320"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HMZ63295.1"
                     /translation="MLIEFSVTNFRSIRDKVTLSMVADKSISKKNELPDNLIEVDKNL
                     HLLKSAVIYGRNASGKSNVLKALFNLVTLVSESQNYSVGQEINLHNPFSFQKGSSEIP
                     NQFEIIFLLFNKEENSKIKYHYKVDIDFEKIRYESLYFYPKGQRAKLFEREYGKEITY
                     GDSLKGNKKNIEDGLLENQLFLSKSSSYRLEILNEVYSYIVKKIFIYLDLDLYEKMQN
                     ENNMKSIYNLLEIQLAKKNLLELLKAADTGITDILTLNDDKLIFTKHDYANSESDFIN
                     SLLFLNEESSGTRRLITIFLPIFGSLITGTIVIIDELDKSLHPLLTKMLIQIFHSKKN
                     NPNNAQLIFATHDSSLMNGELFRRDQMFITEKDEIGQTSIKSLASIKGVRRDIPFEKW
                     YLNGSFGGIPVIYEPEFEFTDNKS"
     gene            complement(34942..36276)
                     /locus_tag="PLF66_04325"
     CDS             complement(34942..36276)
                     /locus_tag="PLF66_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016548720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HMZ63296.1"
                     /translation="MKLEEAVEFMCAEAKRQTADSFDVIGVESDDAGVEVFEGKVKST
                     EIASSRGIGIRLFKEGRPGIAYTEKFSEDAIKQAVTDASAHSRITDKMDLDLPMGEKL
                     LDLDLESYKEEVDHITFDQMIELGLKLEQIALSRDKRIVNVPYLGVSKSSARSIIQNS
                     KGVNYSRKGNAVSAGLGVVAKENESSKMGVYSNGGRSFSIFNTEFMSSKAVERALELL
                     GAEPVESKQYPVVFSNRVSGGIIGMFLSPYYAELVQKGQSRLADKVGSKISVDEFTMT
                     CNPHIPGFPGSRLFDSEGVVTKVTPIVQNGILKTFLYNLESAKKAGIAPTGNGSRSYA
                     GKAGTGTSNLIVEKGSKTVTELLSAYPECLYITKLEGASGCSAISGEISIGAQGILYK
                     NGKPVRPIDRITLNSNYFDLLHLIRGFSNEYNDAFSSVKVPDILVESMYVAG"
     gene            complement(36277..37662)
                     /locus_tag="PLF66_04330"
     CDS             complement(36277..37662)
                     /locus_tag="PLF66_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008589723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HMZ63297.1"
                     /translation="MDKIKAEKIIQAGLDKRADFVEIFVEETRGSSVSYNNRKVETAS
                     AGTDYGIGIRLIYGTEVFYANTSNDEEEHLLQLIEALSKTKSDLKNINSKEIIHLKEK
                     VFPNIHTITLDPRKVGQDKKLSILKLADETARKISPKIAQVSARAFDSVSNVSIFNSE
                     GLEVNDYRVLSRFSISVTAQDGGEMFSAGESPGAQRGFEFFETLNVPQFSETAARRAL
                     LMLSAGYATGRKMPVVMGNGFGGVIFHEACGHPLETEAIRKKASPFCDKIGQQIAHTA
                     VTAIDEGAIPNSWGSINVDDEGYPVEKTVLIEKGILKNYLSDRVGSMETGVRRTGSAR
                     RESYHYAPVSRMRNTYIDKGNDKFEDMISSIEDGLYAKKMGGGSVNPATGEFNFAVEE
                     GYLIKNGKITSPVRGATLIGKGHEILPRISMVGDDLEITAGMCGAASGSVPVTVGQPS
                     IKVDEILVGGR"
     gene            complement(37728..38156)
                     /locus_tag="PLF66_04335"
     CDS             complement(37728..38156)
                     /locus_tag="PLF66_04335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63298.1"
                     /translation="MEEEFQPTKKSGMPYGLIWGVLFLSVYAYLFFFFGVYFIWDQGM
                     PDTFGGLLLLAGSSFVYLIAGILGFVPVFITSIIFIIRDRNLRQSIKNNWPVLLALIY
                     PILPNLPGPIDESVMSVVMFCIRVYLFFRTGKDVKEAAKK"
     gene            complement(38156..38887)
                     /gene="kdsB"
                     /locus_tag="PLF66_04340"
     CDS             complement(38156..38887)
                     /gene="kdsB"
                     /locus_tag="PLF66_04340"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004460752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HMZ63299.1"
                     /translation="MKVIGIIPARYASTRLPAKPLAKIGSKTMIEWTYFHTKKAKTLS
                     DVIVATDHEEIFNCVKRSGGNVVLTSPDHPTGTDRLIEVVKGLREVDIVVNIQGDEPG
                     IEPDLIDGVVNLKLKYRNWEMTSAACPLSKEEQLDPNRVKVVFDKNQRALYFSRSLIP
                     SNFKKTVNVYRHLGIYSYEKEFLLAYNDLPQSDLEASESLEQLRALEAGHSIGIYLTN
                     QAGLSVDSPEDLEAVIEDFKKKGII"
     gene            complement(39092..39616)
                     /locus_tag="PLF66_04345"
     CDS             complement(39092..39616)
                     /locus_tag="PLF66_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004506879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HMZ63300.1"
                     /translation="MGDAAAELDDEEGKPAAEPKTPSPIIKWLIWIGAGIAGVILVAV
                     ISMFVAKQSAVSVYKQQKNIALVKAPPPLETFNFVDEFRINTADVGEAHFIKLKLTFG
                     YDVGQQALGPELATRLPQMQNIINLIISRKTKEDLRTLNDQLDLREEIKAHINHILTN
                     GKIKEVYFREFIVN"
     gene            complement(39646..40473)
                     /gene="motB"
                     /locus_tag="PLF66_04350"
     CDS             complement(39646..40473)
                     /gene="motB"
                     /locus_tag="PLF66_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004506959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HMZ63301.1"
                     /translation="MAKQKCPDCIQKVAEYMLTYGDMVTLLLCFFIMLYTTGKTNQRE
                     MQIILSVFKSSTGFFDGGQTLAKGSLEELGMNVESLPSQTSGKALSKARNQATQVFKS
                     EIEKGRVRITEDERGLVISLIGADYFNPASAILLPPVKDVLKKASGLIRDLDRFVKVE
                     GHSDEDAVLPGTNVGREERTYINNWDLAGARAINSTVYMINVGKLAPGLFQAVSYGSA
                     RPYAVEDQGSPEAKAYNRRIDIVILAEKSVGRKPNESNYGLPNTKLPNTETTVEGED"
     gene            complement(40483..41268)
                     /locus_tag="PLF66_04355"
     CDS             complement(40483..41268)
                     /locus_tag="PLF66_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000345490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="motility protein A"
                     /protein_id="HMZ63302.1"
                     /translation="MDIATTVGIGLGLFLLTFGTIMAGLGAGDVINVPSVLITFGGAT
                     AGTMISASWESTLGIGKVTRKAFFIDKFDIPGLITTLVSFSEKARREGLLALEDDVNE
                     LPDEFLKKGIQLVVDGTDPELVRNIMETEISNIASRHAAGRAWWDTYGGLAPGFGMLG
                     TLIGLVAMLKNMGTGDSSAIGTGMAAALITTLYGSFAANLLAIPFVKKLAKRSEDELA
                     MKQIMIEGTLSIQSGDNPRIVKEKLSSYLAPSERAVLKDDGKD"
     gene            complement(41274..41489)
                     /locus_tag="PLF66_04360"
     CDS             complement(41274..41489)
                     /locus_tag="PLF66_04360"
                     /inference="COORDINATES: protein motif:HMM:NF018043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FlbD family protein"
                     /protein_id="HMZ63303.1"
                     /translation="MITLHRLNNSEIIINCNMIESIESTPDSVITLQNDKKIIVREKA
                     EEIINSIIEYQRKVFKNAIHISANEEK"
     gene            complement(41509..42597)
                     /locus_tag="PLF66_04365"
     CDS             complement(41509..42597)
                     /locus_tag="PLF66_04365"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008590491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HMZ63304.1"
                     /translation="MKVAIIHDWLTGMRGGELVLDSLLKIYPDAELFTLIYNKGTLNE
                     RIENRKIHTAFTDRLPLKAKKYRSFLPLFPMAIETLDLRGFDLILSSSHCVAKGIIPS
                     PDSIHISYIHSPMRYVWDLYYDYFPAKSGLKFYMIQAIANYLRAWDVASSHRVDLFLS
                     NSKFIGKRIQKFYGRDSVVVHPPCLPEGYKVVESPKEDFYLIVSAFAPYKRIDLAIEA
                     FRENGRKLFIIGGGQDEKKLKQSVPSNIQFLGGLPREQIVEYYKKARAFIFPGLEDFG
                     ITPVESQGYCTPVIAFGQGGALETVIDQKTGVFFPEQTVSSLNDAINRFEKLTFSKAN
                     FQKNINRFTEEKFINEIKNQVDRQIREN"
     gene            42757..43272
                     /locus_tag="PLF66_04370"
     CDS             42757..43272
                     /locus_tag="PLF66_04370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63305.1"
                     /translation="MSNAPNVKYNSDNDFYYFLAVLDDESVKTTLLHFNLTEIINMSN
                     QIKKINFDELEKEEIVEFLLTTFNEKCGTDPAKILKIGQRFQITDNAINTEKKNFINT
                     LLKNLKSRTPNFYKSNIRNLKKKKDLNEEDSRKLGMNEMANKLIHDNKKEVIIILKAL
                     YTFKRFENIQL"
     gene            complement(43553..44998)
                     /locus_tag="PLF66_04375"
     CDS             complement(43553..44998)
                     /locus_tag="PLF66_04375"
                     /inference="COORDINATES: protein motif:HMM:NF022525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2779 domain-containing protein"
                     /protein_id="HMZ63306.1"
                     /translation="MQGLKCDKLLWHQYHSEITPTYSAKNESNLQRVNIQNEFIFKYA
                     KLIYPNGKEVPRNHSMSSSHKMTQNLIAERSIVYNASFLTSDFFSRTNILIPSSIDDS
                     WEIIEVKSSINLKHDNILDICFQVHVARQSGLKISNCSILHVNPDYSFTGELDISQLF
                     ILKNVTDRVNSAKSQFEKQVNYLKSLITRLDSPDLSKEHSCGTPKNCILKTCWNDLGE
                     GNLFNLREGGDLTTKFFNLGVRYVKDIPEDPDLTFTQKVQIESERSNSPFLQKDKLTE
                     FLNSIQFPIYFLDFETINPAIPVYVNSKPFQHIPFLYSLHVMDNKDLPPNHFSYIDDG
                     SVDPREKILDDLSKIISIDGTILCYNAVFEMKCLRESVKLFPQYSDWYSSIANRFVDL
                     SNPFRFFYYYHPKQNGSASLKSVLPALTGLDYKELEINDGNLANLEFLRAKTMNLPEE
                     ELTSIYRLLLDYCKMDTYAMVQILNTLWKLI"
     gene            45179..47365
                     /locus_tag="PLF66_04380"
     CDS             45179..47365
                     /locus_tag="PLF66_04380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63307.1"
                     /translation="MNLVFVLIYLLPLLLFAESKIQKSPKSTKVKTGILFQSNKNIQI
                     TLPTESGFRKDLYDINGNLLESEFIDSNGNLKEEEPKVAIEKRIYNSTGFLETIQTFD
                     KGKELSSETIFTYDKACLVSQKNSECVNEISFLDENKNPIEDENGIHKYKTEFDLKCI
                     SLPNRKPSECKTLEANFNKKQKLVADNNGYTKVIRTFDDRGNLNLLEIYNIKNKIKQK
                     AIYAYDYKCIEITGKAQGCIIFHEYVNELEEKTKNTNYGKKILQYDQNCLMEKKNAEK
                     CIALEENYDLNGKLQDIAHRFTFGCMDYSLELGAYAKKISKFDKKGNEIVREFYNSKN
                     QLIEDSSGGAKYIFAYNQECIDTGNKSHLCRTLTEVYDKNSKLKYKAIFDENCVKSGK
                     AIFETPNYCTAWKEIYENKQTENNKNSIQIIKANFDTNGFKINLEQFNINHKLSLNTK
                     YYFDKEKLIKKENLDINGNLREDEDGIATYVYQYGNGKYTIKEETLGKDGKLKANSDG
                     VARMIFSYDENCLKKKSNRYDCTTLEEFHDANENLIEVKSNKEDNYTYAKLLREFDQS
                     GKLILEAYYDLKHKLKKNSKGISKYKFHYDSNGEMILEEQFGKKNSLKRKIQFEYDPK
                     CKSISNEFTCNTSISHLNKRNELIDKENLDGVPYAKESSSYNHECLKILSEPEDCLQK
                     KEFTNSKNQIIYAEYYKYFKIEKSEIKMIRLQLTQDKIPYSAEFIK"
     gene            47408..48598
                     /locus_tag="PLF66_04385"
     CDS             47408..48598
                     /locus_tag="PLF66_04385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63308.1"
                     /translation="MIDFIKQLFGIQSKYEPHPDAVIISCFFNPQNSYYRLKAFRQFY
                     ESIQHTNHFILECVIGKANPQLPDSKFIKRVYTENLLWHKESLLNKIISDHIEKDFKY
                     IFWVDADVLFTNPNWIVEGVDELERHNIIQPFEYCIHLEKNETKPTFNVDEHRKKIYD
                     INYSNRRLWRSFAANYKTANLNFAQNENYNIHGHVGFAWGAKREIITKVPLYDRALIG
                     GADHIIAHAATGQIPHSCITKSFTDNLEEVLTWSKKFFQQTKGKVGFVKGDLYHLWHG
                     DIDKRQYLKRIQDFTAKTKQIVHKDENGLYITQSGDDEYMKKYFKHREVMSDNDYLSS
                     MGIHYMTNSDRNDSTEDAIIASSFLDNEKEITGGGGEMGGAGASGSWDDNSSSHTAQS
                     DNFS"
     gene            complement(48608..49048)
                     /locus_tag="PLF66_04390"
     CDS             complement(48608..49048)
                     /locus_tag="PLF66_04390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63309.1"
                     /translation="MKQFLILALLFTFNFTSGIFSLECPKNLTVSFVSPKENEAVNGA
                     KQKVTLSISQKDGLFYHLIQSNANDKKKINYLQGSGAINNLNINSNVWLGNDSQGKNS
                     SFVITAIVSTKLIPKEGKVVEWNDSALKPTEICNKTSITVNRAN"
     gene            complement(49231..49449)
                     /locus_tag="PLF66_04395"
     CDS             complement(49231..49449)
                     /locus_tag="PLF66_04395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63310.1"
                     /translation="MSERGKCREELAETSNPWAGDVGRCNLNQPLVQSGRPEDKNIRD
                     AAIINCFAARMRVQNCNSKSETIPSLKN"
     gene            49718..50968
                     /locus_tag="PLF66_04400"
     CDS             49718..50968
                     /locus_tag="PLF66_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010573489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HMZ63311.1"
                     /translation="MMATISGRIITHENDIIGTISYDPTTGLITDIQEGIKENSKVYD
                     TSSCIIFPGFGDIHIHAREDVSGKHNYKEDFISASQAAVNGGVIHVADMPNNPVPPID
                     ETSYEEKSKLTQKSPIHITLYAGIGPNTNPLKKSVPYKVFMGPSIGELFFKTNEELEE
                     VIKRYAGENVSFHCEDPIVLEESKGAATHEDRRPLKAEILATDFALYLIEKYNLTGKL
                     CHYSSGEGLQKIIAAKKRGVKVTCEVTPTHLFFDRSVLTEENHKWFQMNPPLRYETDK
                     NILLAAFKEGWIDYLATDHAPHSIEEKLKGISGLSQLDTYSLFVTHLIVNEKVDLKRI
                     AETCSQKPGDFVNPYLPKEYGKGFGYIKKEYSANFTVLNLKKPTMFRKENIKSKSGWS
                     PFENHTFPGSIEAVYFKGNLLDRG"
     gene            51004..51333
                     /locus_tag="PLF66_04405"
     CDS             51004..51333
                     /locus_tag="PLF66_04405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63312.1"
                     /translation="MDTLKLILMRILISWSWFLSLFIILWLRKKQFFKKSDSAVFTLW
                     ISLFFYSIFVFYFHLNTLLIPDRHGHAYSIIAFAFGIPLILYLIIIPISIFIAMVASF
                     IIGRFKN"
     gene            complement(51330..51638)
                     /locus_tag="PLF66_04410"
     CDS             complement(51330..51638)
                     /locus_tag="PLF66_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016543615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HMZ63313.1"
                     /translation="MLATFPQDIQTSDDTLTITWKDGFVSHYNLLDLRKKCPCAVCCG
                     GHGGTIGAATGHINEIKLISWRKVGRYALNFTWSDHHDSGIYSYDNLRAYSESKAELS
                     "
     gene            complement(51644..51793)
                     /locus_tag="PLF66_04415"
     CDS             complement(51644..51793)
                     /locus_tag="PLF66_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008594055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63314.1"
                     /translation="MNQKTVKLLKKYADLKGIDKKQIKREWLSMNESEKDIKRQEILA
                     VLVKK"
     gene            52013..52372
                     /locus_tag="PLF66_04420"
     CDS             52013..52372
                     /locus_tag="PLF66_04420"
                     /inference="COORDINATES: protein motif:HMM:NF020397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HMZ63315.1"
                     /translation="MNEIDKYIKKFPAEVGKKVAHLREIVKKVAPKAKEVYEHKVIAF
                     TIKDDYLIWYGVYRNRLCIFPKSETIEKFRSELDGIIVTKGTIEFQASKPLPVDLITR
                     ILKYRLKELSKSNQDEK"
     gene            52617..53201
                     /gene="rdgB"
                     /locus_tag="PLF66_04425"
     CDS             52617..53201
                     /gene="rdgB"
                     /locus_tag="PLF66_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004457281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HMZ63316.1"
                     /translation="MNPTQLALASNNQHKISEFKKILSSGFSLLTPKELGIEFEVEET
                     ESTFEGNAKLKSEHLFKISGIPSIADDSGICVSALNGKPGVYSARYGKQGFTDRDRTE
                     FLLSEIKGNPDRNAYYYCCIAFTNKEGTRFFSGKCEGLLADDYDESGNGFGYDPIFIY
                     PPLNKRFSQISAEEKNKVSHRGIALREFAKFLIQ"
     gene            complement(53246..54424)
                     /locus_tag="PLF66_04430"
     CDS             complement(53246..54424)
                     /locus_tag="PLF66_04430"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HMZ63317.1"
                     /translation="MTEESCKVLIVDDNEEYRYALELIVRSLGHESIHAADGFEALKA
                     TLENVPDLILLDINMPNMDGIIALQKIRRNPKLRSVPIIMISTLLENETVLECLEAGA
                     DDYIVKPFDKAILKARISNNLIRRIYQKKEKEILEKTLIGSIHILTEILETLDSKVFS
                     RIARIKKHIKYITEELDLDEDKWVMDMATHFYMIGCIHLPRDFVLKSITGRMLNWDEK
                     KIFEQHVLHGYKILSKIPRLERVADVVRLSFKNYDGSGSPEDIKVSGEAIPVESKILR
                     AAWEYEMISIRNDSPEATIASIKTLSGKVDPNLLPYLEKLYYLEQTGDVIEVSLPSLS
                     VGMILMEDIYTKNAVKVAGMWQEVTESLLDRILSIAITFGLKEPFKIIFPKSNVKQKD
                     "
     gene            54961..56208
                     /locus_tag="PLF66_04435"
     CDS             54961..56208
                     /locus_tag="PLF66_04435"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HMZ63318.1"
                     /translation="MIKEKVLVTDDDISIRTLLINYLTKLNYEVHSAEDGNDALSKIQ
                     TQQPPFNLLITDLDMPGMGGKSLIQQVKDLYPDLIIMVVSSHDESKIIIDIMRMGVFD
                     YIVKPINREELQLKATRAMSAALLKKDNLIYEKEKTLRLENQINWFNYKEQSKSNELF
                     SQEKLKQNLFTNLKTHLGQGMGFGLLTSVSEMILMCPKDEKGNYLISPDIVEILEINA
                     NYARNVISYFGGIEKIFDDGILLQKLPISTVYQLTEQIVKKQANNADSSHLKIILSEG
                     NFSDNVFIRTNSSYYDEVISELLINAIKYSEQYSQISILFSLESDKLKITVLNKPLKY
                     GKKEAEGIKEEYLNLIFEPFFRIEKAITNESMSLNYGLGLTKVKIILERMDATIQVSN
                     FNDYSGKSPSIKVGFTISFPIVS"
     gene            56243..57037
                     /locus_tag="PLF66_04440"
     CDS             56243..57037
                     /locus_tag="PLF66_04440"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HMZ63319.1"
                     /translation="MIIETKHHIQILEPLNPIKTLVFINGFGTEQDIWSEVIKPFKEN
                     YKIILFENIGSSKSDLNYFSPNKYDSLNAYSKDLNIISKKLNLENSILVGHSVGGMIG
                     LLSTIQNPNYYSKLVMIGSSPRYLNDSDYYGGFEKKELENLFESMRANYFAWASGFAQ
                     ITMNTPENPKLSEYFAASLKSIQADVAISVAKTIFYSDHRNDLAKVKIPTLILKTRED
                     IAVPPQVSDYMQSKITGSRKIEVNAKGHFPHLSSPIEVIHAIESFL"
     gene            57143..59407
                     /locus_tag="PLF66_04445"
     CDS             57143..59407
                     /locus_tag="PLF66_04445"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HMZ63320.1"
                     /translation="MNIDSIENLFIKFWRELLDLGVDDDSKLLEKYIEILHKYSNSKG
                     SIFYQRIDHITVRNIISHPKEFNIDLEEEFFYIFDSKNKIEILEAENFGFTGNLSLII
                     LPILSGEKASAFVLLIEKESISKDFLFFLDNANAALAQRFIANRMLEDIALKKHQSNI
                     IMQTIPEAVIYFEENGNFVWLNNLAKNLLKIEGEFLKPDIVSSAMIRLRNAAINSDEI
                     NQVGNRIFQEGNSEIKNWYWIYGEPVDLVYQVSVFKTAYKNSNGQIWVFTDITNRYLA
                     NERLLELNKEIKEKIILAENANNAKSTFLANMSHELRTPLNSILGFSQILLQSNEINN
                     NDKEHIRFIQKSGEHLLSLINDVLDLSKIEAGKFELQFSIFSLHSLIFTIGKMLEIKA
                     IEKGLGFEVIISPNTPSFVIFDERKLKQVLLNVLSNAIKYTDKGEVVLSLNLIRQEGN
                     KARISFSIQDTGRGIAKSDIESIFMPFQQITTNSQYVQGTGLGLSITKSLVEAMGSEI
                     KVESELGKGSQFSFEIEFEIPENNQEEAISFDEVIGYKGNRKKILIVDDNIMNRILFE
                     RMLRGIGFLIEHGESGKDALSKIDIFFPDLIFIDLRMPGISGLDLIKIIKDNPKYKDI
                     KLIATSASAFNETRNKCIEAGADDFIIKPIQFSELITKITPCLKIEWIIIESKHPQVE
                     DNKNIPLSIDKNLLNQLLDSAKQGEIATIETNLKFLKEKYPDQNPIWEKFEKYTNHFE
                     INLLIESLNQLIGE"
     gene            59410..60552
                     /locus_tag="PLF66_04450"
     CDS             59410..60552
                     /locus_tag="PLF66_04450"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HMZ63321.1"
                     /translation="MSLDAFAGAKILIVDDEPANLRVLYNLLKDYPLRLFTLQDSKLV
                     MNTVEKELPDIILLDIMMPGINGFELCKMIKKKDKFSGISIIFISALNDINNMVKGFE
                     LGAVDYISKPFQNEEILIRLKNQLTIQFQKKDLTKLVEDLEQKNQKLIELDSMKNKFF
                     SIIAHDVRNPFAGILGLSEILSSDFDTLSKEEMKKLAEMINSSTNKCYKLLENLLNWA
                     KSQLNNVEIRPIKKNLKHHVEKIFSVYQLQADNKNITLNSSIPEDIFYYADENLVDLV
                     IRNLVNNAIKFTNESGSIEIYCKTQNDLLEISVKDSGIGMPPSISENLFKLGKSVTRK
                     GSNKEDGTGLGLLVCYEFIQKSGGNISVISEEGKGSIFTFTVPCFK"
     gene            60723..61499
                     /locus_tag="PLF66_04455"
     CDS             60723..61499
                     /locus_tag="PLF66_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HMZ63322.1"
                     /translation="MIEIEKNPIKNYRKLKCEECINGNMLDFQFSMAFQPIVDTRTKT
                     VFSQEALVRGVNNEPANFILSKINDENRYSFDQSCRVKAIELASRLGLKSKVNINFFP
                     NAIYQPETCIQTTLEASQKYGLPISSIILEITEGEQVVNHSHIVSIFKEYRKQGLLTA
                     IDDFGAGYSGLNLLADFQPDFLKLDMLLIRNIDKDKTRRIIVNSIVKICQEINISVIA
                     EGVETKSELDCLEDLGIFLFQGYYYSKPSFEALTTPKDFQ"
     gene            complement(61537..62028)
                     /gene="ispF"
                     /locus_tag="PLF66_04460"
     CDS             complement(61537..62028)
                     /gene="ispF"
                     /locus_tag="PLF66_04460"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010573650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HMZ63323.1"
                     /translation="MPPKYRTGNGIDFHQLAVNPDRPLILGGYTIESEWSLIGHSDAD
                     IVLHALSDAILGALGLGDIGQYFPDTDKSLKNMDSSIILKKCLELMRERGYKLLNVDN
                     TIIGEKPKIAPHRINIISSLAKLLEIPVDQVSVKATTTEKMGALGRSEGLAVFSTVML
                     ELI"
     gene            complement(62012..63601)
                     /locus_tag="PLF66_04465"
     CDS             complement(62012..63601)
                     /locus_tag="PLF66_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015680012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HMZ63324.1"
                     /translation="MVSMALSLKEETSRRRTFAIIAHPDAGKTTLTEKLLLYGGAIQL
                     AGAVKARKDRKSATSDWMAMEKERGISITSAALQFEYKNFVLNLLDTPGHEDFSEDTY
                     RTLIAADTAVMVLDAGKGVEAQTRKLFKVCRDRGIPIITFINKMDRPTKDLFALLDEI
                     EEVLGITAVPVVWPIGTGPDFKGVYSRETKQLNLFQKTPGGAYQAPVQLSGITDVSLE
                     EQIDSDILQKFREEIDLIENGISPFTDKDFLGGVITPVFFGSAVNNFGIQLFLDSFLE
                     LAPPPVHIGLRDGGELDPINSPFSCFVFKVQANMNKVHRDRIAFVRICSGKFERGTTV
                     NHERLDKPIKLASSFAFFGQDRNTIDEAYPGDIIGLVNPGTYKIGDVLASGPIPKLKP
                     LPVFAPEIFATVSCPDSMSLKSFKKGIEQLSEEGILHLFTSRTIGSGYPIVGALGKLQ
                     FEVFQRRLLDEYNAKSNITSLPYGACRWVKKEDEPRIPSSTNQIFDRDGRMAVLFETD
                     WELNYFKKNNPDIELLDAPQV"
     gene            complement(63819..64481)
                     /locus_tag="PLF66_04470"
     CDS             complement(63819..64481)
                     /locus_tag="PLF66_04470"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HMZ63325.1"
                     /translation="MIAKFPDYYNTLNLPFGSSPDVIKKYYRELAKIYHPDNKRTGQK
                     EKFQTLLAAYQVLTGEFREEYDKAYLKNLTLISNAEKLKKQFILPQERLIYTTSMTFL
                     ARRGLLRAGYRRKDRAKFTGLNHDLDLLIKKEETVNKIIVKVPLTVRILCPECLGSNI
                     YCECCNGIGTYKGTRILQIGFEPNLLVHNRVYEFELSRFRPDKFIHFKKKKLRVRIDI
                     IQ"
     gene            complement(64508..>64940)
                     /locus_tag="PLF66_04475"
     CDS             complement(64508..>64940)
                     /locus_tag="PLF66_04475"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002971745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HMZ63326.1"
                     /translation="RNRTHPIDACLASLEIQSLMNEIKELQDSLGFEFWELRIGIHSG
                     PVVAGVVGQKKFAYDIWGDAVNTASRLESSGQSGEINISKSVYDNIKDFFVCEYRGKV
                     HAKNKGDVDMYFLKRLKPELSQDERGIIPNENFFEMYRTYL"
BASE COUNT        22524 a        11190 c        10392 g        20834 t
ORIGIN      
        1 atccaacaat atttaataaa aatatgatag aaatttcaat ggtagattgg agaaaggtag
       61 gatgaaagcc caaatagcta gaatagaaat aaagaagcca atactggatc aaagtgatcg
      121 ataccaatgt gatcgatgta atgaatcctg ggcttttaat tttatctttc catttcataa
      181 ccgagagcaa gtggtatgaa tttagaaaaa acaagttcta ctgtcaaaga aaattttatt
      241 gtatcgaaaa tttatggagc ctactacgaa atttattctc caaagcttgg cttcaaacgt
      301 gccatattaa aagggaaact tcgtttaaat aaatcagaag aaagacatcc ttttgtagtc
      361 ggcgatcgta ttgttgccga aaacgagtca ccagagagag gagactggat aattgagtca
      421 cgggaagaac gtgtcagctt tctaactaga cagagtagta gttccgacaa acacatatta
      481 tgcgcaaatg ttgactatgt agcaattata gcatctctat caggacctga aacgaaagat
      541 ggatttattg atagaagtat taccgcttgc tatcattcaa acgttaaacc attgattatt
      601 tttaataaaa aagatttagt aacagaggaa gttttatccg agcgtgtcca aaaatataaa
      661 gaccttggct atcagatttt tccgatcaca tgcacagact tggaaagtat taaagatctg
      721 ctcgcaacaa tacaagacaa aacgacatac ttagtaggaa actcaggcgt aggaaaatcg
      781 agtctaatta atctaatcac aaacagcaca gaacagcgga taaattcaat tagccactca
      841 acccaaaaag gaaaacatac aactacaaat tcaagtgtaa ttatagtaaa tccgacaacg
      901 attttaattg attctcctgg aatcaaagaa tggggattgc ttcatctctc aaaaggagat
      961 atactatcca gttttcccga aattaataaa tacaagaaaa aatgtagagt tggaaattgc
     1021 tgtgatgcag gtaataactg tttaatgatt caaaagctag ataaattaga tgagaataga
     1081 gcaaaaagtt tagagtcaat gattgaaagt ctagaaatcc catatagaat aagagtaggc
     1141 aatcttaaaa gtggaaaatt acgaaaaata aagagtcatt gggactaatc tacttaagcc
     1201 accaaacaac ctttcctttt gtattaatat atccccagtg ccctccaaag acctgagcta
     1261 tacccagacc tcttctaaaa ggtagcacat acatataaat tggttccgtg agttcttttc
     1321 cagactcatc cactaacgca tagtaaatga tatgtccgtt atttttcttt ctatatcgaa
     1381 gacttaccca ggaaacgcca tccacaaaat tacctttttc gaatgaattt gctggataaa
     1441 taatattacc ctctctatcc gcaaatcccc aataatctct cacttgacca tcaaaattat
     1501 ctttcttcgt ctcggcaaac caaagcaact tatctgtatc atttccaaaa aattgttttc
     1561 cattgatttc gatcgaagtc ttttttcttt cttcttgggg ggtaatttct gttagactct
     1621 taagaaatgg ctttaatacc cattcccctt ttctgttaat acgcccccat agagaagtat
     1681 gcgtcttacg agcgtaggca agtccgtttt gaaacatttt tacttggtaa aatctctcac
     1741 ttgtgacttc cttaccatct ctatcaagaa tttgaatcag aactcttttc tcatccgaat
     1801 cataataact cgctgcccat aatacttcct catcagaaac taacgattca atcgagcgat
     1861 aggtgggttc aagtataatt ttacctgcta tgtcaatcaa gccgaagtaa attccttttc
     1921 gattgtgaat ggcaaaagga gctacatcat tttgaaagaa tccaatttta tggtattcgg
     1981 gagcaattaa ctctataccc tctttattta ctactccata ctttcgaatt cgattaaatg
     2041 tatcacctgc atcaatataa accttagcta gaccatcctt tccaaaaaaa attttctctt
     2101 tgaataattc tccagacatc cttccaaaga ttctatttat ataagggctt tcttctgaca
     2161 ttacgccatc atatttggga ggaataagta ttttaccagt tttatcgact aatccccatt
     2221 tgcctctttc acactcaccg acaacccatt ctttgcattg atcaatcacc caagcaagcc
     2281 catcctcaaa ctctcttaca tcatcaaatt gtggttttaa tataaactca cctttttgat
     2341 ttataatgcc ccatttatca ttcatttgag caagagctac gttatccgac attgaaccta
     2401 aggcttcata tttaggagga atttttacat ttccttttat gtccatgaag ccccaatgaa
     2461 atttcatatg tccaacaagc ttattgatcc taaaaaattc gtctccgtaa ataggaaaaa
     2521 ttaaagagat aattataagg gagaaaatta acttaaacat ccatgaagaa atgattccca
     2581 acaatgttca ttccttttcc aagatataac ttatattgag catcactata tagaaccgac
     2641 tattgtgatt gatatattgg tttcaattcg aatatctttt aaaaagatcg tataacgtcg
     2701 aatgtggaca gtatttatca aaattaaata ttttgattct aaagatttta tcttcattcc
     2761 tttctatcat attcaactcc aataaatgtg atatcatctt gtctggaaga attttctaaa
     2821 aattcattta gatttttcat aatttcttca aacaattctt ttaaatcttt atcactatat
     2881 tcctttatac tacttaaaat ttgtttttcc ccgaattcct ctttttctga attaaactct
     2941 tcaaaaagtc catcggtaaa taaaagtaat ttatctcctg attcaaatcg aatttctttt
     3001 tcttcaaact ctgcatcttt cataataccc atgatctttc ctgttctaga aataatttct
     3061 aaactccagt cttttctttg aatcacttga tcaggatgcc cagcagaagc gtaaatgatt
     3121 ttattttttt caggataaat gtctactacg atacaagaaa aaaaactatg tatagctcca
     3181 aatttttgaa caaaatcgtt atttaaattt ttaagcagtt tcttggggct atgagtagta
     3241 tgcttaaaac tttcatattc acctttaata gccattgtaa ttaaggcagc ttgcactccg
     3301 tgacctgtag catctgccaa aaaaatacga atgcatttat ggcttaattc tgaaatatca
     3361 taaatatctc caccgacctc atcaagtgga atataattca cggagaaatt taaatttttt
     3421 atatgtgcaa aattcttttt catcaaacta gtttggattt tttttgcgac tgaaatatcc
     3481 ctttttagat catctagtgt gtcatttatc tcttttgttt tttgatcgat agtttttgat
     3541 tttaaaaaat catctagttt ataatgaaag aaaattagat ttagactgta agcaaaaaag
     3601 atagatacga cagaatgaat tgcatttgag aaaaacaaat caggattagt ctgaaaatta
     3661 tacaatccta aaataaaaag aaaaagaata gaaaaataaa ctaatgtcgc atatcgttta
     3721 aatgtaacaa agagcgcagc aataaaaaca gctcctgttg taaaaaaaga aatatttcca
     3781 gtcaaacgtt gagaagctag agaggataat gcaaataaac ctaaaaaaga aaagatcgat
     3841 attaatataa atccctctaa taaaacttta ttccacttag caggatttaa aaacctataa
     3901 aaaattaaat ataccaatgc aaagaataca cctaaataat gtgctccaaa taaaacttcg
     3961 caataataga gatataagtt acaataatta tgtgaaacga tttctatatg tgtccatacc
     4021 ataaaaggag ataaaagtag aataaaaaag gaaactagat taaatctaga gagattatcc
     4081 attcgtaatt tatcagcgaa ctctcttcta taacgaaatg gaacatttac ataaatggtc
     4141 ttatagattg aaattagatt ctttatcatt ttttctcttg ttgtaatcca aggaataaga
     4201 atgtcgatcg tattcctttg ggttaaattt caatcaaata ttaccttatc tatatattag
     4261 acgaatggaa taagccaatt taaatcatga attatgcaaa gtcaacactg tcattttttt
     4321 tagtttttga ccaaaaaagg tctattttag tattaatcat tgaccaacta tcggaaagga
     4381 tgaagatggt atcattctta aattaccttc ttttaaaaaa aatgaattta aatgaaaaca
     4441 ttgaaataaa aatagatttc tctagtttag aggaaatata cggggaaaac aaaaaagact
     4501 tttccctcat tctacagaaa attcaacagg aatacacaaa gtatttagct actttagaag
     4561 aagaaattag aacagaaaat ctagacgaat ttcgaaaaac tcgacataag atttcagcta
     4621 cccttctttt attaaaactt gagaaatttc atgaatatct tacatttctg aaagagcatt
     4681 ttgctcatgg atttcatgac aaagaagaat caatagctat tttaaaagat tgtttcgcaa
     4741 aaacacaaag tctaatagca agtaaattag aggatttatc ttctcaaatt cactgattct
     4801 aaatgctata aaggcttctc atgaaactaa aaaaaattct cattctactt tttctattac
     4861 agattgtttt tactttaagt atgaaaatct tttcctacat tggagatgat tcacaggaac
     4921 tgcttactag gactcttcag tattttacac aagaagatat cgataaagga attgaatatg
     4981 gacgtagagg attttttgca aggatagctt ctttactcat taactttatt ctgacaggta
     5041 tattcgtgtt ttccccttta gcaattcgcc tagaagagta tctactacat aaagcgaata
     5101 accgctatta tccaatgatt ttcctattct tccttaccta tactgccata gaatttataa
     5161 tttctctccc tcttcaatac tactttggat tcgtattaga acataaattt ggtttttcca
     5221 atatgacttt tatggactgg ttaatctata ctggcaaatc agtattagtc ggactagtcg
     5281 taggcagctt tctttctatt gcagcagcct ttattctaaa gactttcgaa aggacttgga
     5341 aatacatatt accaattggg tccttagttt taggattagc gttctctgtg cttgcaccaa
     5401 tcctaattac accgattttt tatgattata agccaattga agaaggaagt cttagaacta
     5461 aaattatttc tctttgcaat catgccaaaa ttactgttga aaatgtttat gtaatcaacg
     5521 aaagcaaata ttcaggtcat acaaatgctt attttactgg atggggagaa aatagaaaaa
     5581 tttttctata tgatacatta ataaaaaacc atacagaaga agaagtaatt agtgttttag
     5641 gtcatgagat aggacattgg aaatacaatc atgaactaac gtttcacatc ggtaatacaa
     5701 tagagttgtt tttcatatgc ttattactta gttatatttt caaaaaagta aaagaagaaa
     5761 atgaattcaa gataaaggaa atttattctc cctctacatg gccaatttta tttttggtag
     5821 tttctatcct tggcactatt acaagtcctt tctggaatac tttcagtcgt tatcaggaat
     5881 cacaagcaga tatggaagcc ttagttttaa caaatgataa aaaatcattt attgatactg
     5941 agataaaaat ggcgagggat aataaatcta gattaaatcc tcatccattt acagtttggt
     6001 attcttattc tcatccaatg acaattgata gaatcaaaat ggcagaagat taccaaccac
     6061 aaaaataagc catgagcaat atagaatttt tagaaaatct aaacgtagag ttaaacgatc
     6121 atacacttcg agaaatgatc tttaaacgac tcatgaaaga atacggaaga gttatacaaa
     6181 gagaactaaa gaagaatcca aaaacgttat ccgtaagaat gagagatggt catacctacg
     6241 aagaattcct aactcaacaa ttggaagatg ttatcagaat cattggcgaa tttacgcatg
     6301 gtccatcttt ttttgaaaag atttacgtag aacatttaga tgatggcgaa tttgagaatg
     6361 taactgcaaa aaatttaatt cttacttatc gatatcgtat agagagcttt ctatactcta
     6421 ctaattttga aatggataaa aaactcagaa cagctataga gcatgtcgta tctagattag
     6481 tagaaacacc cgattatatt ggtaatttta accttcaaga aaaacatatc cgaacagaaa
     6541 tgatcgcagc cgtcttaaaa gatggagatg atgaattcaa aagaaaagca agcgcattaa
     6601 ttgaaaaaca atctctaatt gtaatgaaca caatgaatga aatctttgtt gagcgattaa
     6661 caactactct tctagaagag aaagagttaa agcacgaatt gattgcaaga cagaaagcta
     6721 tggatgaaga gcttaaacga gctagaaaga tacaaaggaa tctattacca aaaagttttc
     6781 cgtctaacgt aggtttaaaa ttctatgcta gttatatccc tatggaaagt gtcggcgggg
     6841 acttttatga tatacaagtt tttccaaatc cagatgaaat cccattagat agaatcggag
     6901 tattaattgc agatgtatct ggacatggag tccctgcagc ttttatagca tcgatggcaa
     6961 agattagctg gcaaaataat tttgagcaat ttaaaacacc ttctgctatt ttagaaagat
     7021 tgaatttaca gatggtagaa aatacagctg gaaatttttt aacagcattt ctaggagtct
     7081 ttgaaacaaa agtgcgacat ggaaatctcg aaatggaaac tcctgacaca aacaaacgtg
     7141 gtgtttttca ttatgcttct gccggacatt tttcacctta tattattcga aaagatatgg
     7201 attctatcac attgaaagta aaaggccaaa tgttgggcgt ttttcaaaat attcgaattg
     7261 aagaatattc aatagattat tatcaaggtg atagatttat tttctacaca gatggacttc
     7321 ttgaaatgaa gaattataat ggagagattt taggggaaga gagactcttt caaatcttca
     7381 gtgaaacgaa aaacctaaaa ggaaatgaga gttgtagcta tatacttgaa aagctctgga
     7441 atttttcaga aggcaaaaca gtagaagatg atattacatt actaattata gatgcagact
     7501 aaaggaattt tatgaaaaaa agaagacttg gtaaatctgg aatggtagtt tctgaaattg
     7561 gaatgggaac aatgactttt ggttctagtt gtgatgaacc gacaagcttt aaaattttag
     7621 ataaagcata tgattctgga attgattttt atgatactgc tgaaatttat cctgttccac
     7681 cagaagataa atgggtgcat cgcacagaag aaattgttgg aaagtggtta aagacaaaac
     7741 caagagattc cgttttaata gcaacaaaag tttgtggtcc gggtcatgga tggttctcac
     7801 ctcctgttcg taacggcaaa acagcattag accgtcacca tataattcgc gctatcgagg
     7861 gaagtttacg tagacttggc acagattatg ttgatttgta tcaaacacat tggcctgatc
     7921 cagacatgat gtatgaagaa acgatgtatg cactctcaga attagtcgca agtggaaaag
     7981 tgcgttatgt gggatgtagc aacgaaactc catggggact tatgaaaagt ctctgggcat
     8041 cagaaaaaaa tagactcatt cgatatgaaa gcattcaaaa taatttcagt atgttaaatc
     8101 gtcgctttga agatgctctc tctgaggttt gtaagaaaga gaatatcagc ttactaccgt
     8161 attctccatt ggcaggtgga gttttatcag gaaaatacaa tggtggaaag gttcctgata
     8221 atgctcgctt tacacgttat gcaaagttag gcgatagaca aaagaaaatg tcaaatcgat
     8281 tcctaaatga ttttactttg aaagcaacag atgaattttt aaaaattgcg aaagaagcaa
     8341 atatgagcct tgtaacaatg gctgttgctt ggagcaaaca acatgacttt gttgcttcca
     8401 cacttatagg agctaataca gtagaacaat taactgaatc tctaaaagca acagatgtag
     8461 tattgtcaca ggaaatttta aataagatca acgaggtttc caaaaacatt ccatacccaa
     8521 tgggttgata taaatgcaag aactcactga atccattgag ttatctcaga caaagattat
     8581 tagcctcaat tctcaacttc agggaattgg ggataatact aatcttagtt ttagatatac
     8641 aaatattgga gatgaagtat cctatgaagc acggggcaga ggaaaaaata aatacgcgaa
     8701 gatccactct gttataagaa acaaacccta taacccaaaa tgcaaacatt tcggagattg
     8761 cggtgggtgc agtggtcaac acctagacta ctcgactcaa atacaaatta agacaaatcc
     8821 aatactcaat caatttaaaa aagagtataa tctagattta gagttgatag aagcagatct
     8881 taaatatcag tatcgcaatc gaatggattt cgcagtattt ccaaacttca tcggtcttcg
     8941 acaagcagaa aatttcagaa ggataatcga tgttagccac tgttctctcc aatccgattg
     9001 ggcaaatgaa gaactagaac aactaagagg tctcatcttt gaggagtatc ccaatctagc
     9061 tttcaacaga aaaacaggag aaggttattt aaaatatctt actcttagaa cgagtgtgga
     9121 tttaacagat acaattacca tttttacttt tacagatgag tttcaagaaa cagaattaga
     9181 atctaaattt gcagaggata ttttaaaatt ttcctcttcg aataatatta ttttttgctt
     9241 caatcgaaaa caatctgaag tttccgctga gggaaaattt aaaatcctga gaggaaaatc
     9301 ttattacgaa gaaacctttt tcccaaaaac aattcaagtt ccatttaatt ctttctttca
     9361 accgaatcca caggggttta tgcctattat ccgctttatt gaaacgaaaa tagacgcaat
     9421 caaacgacct acactcatag atttattttg tgggaatggt tttttcggaa ttttattcgg
     9481 tgaaaagttt tctaaattgg ttggttatga ttgggtagaa agttcaatcc aaacagcaag
     9541 agagaattta aatcgaatct atccagaaaa agaaattctt tataagaaaa aagatttaat
     9601 tcaatttaaa gatgtcttct ctgactctaa actcgaagat tcagttttga ttttagatcc
     9661 tcctagaaat ggaattggaa tcaagctttg cgaatttata ttaaactcat ccgttcaaga
     9721 aatcatttac gtatcttgta atccctattc tcaactggaa gatcttaaaa ttctttccca
     9781 aaagtttaaa attttatctg gaatcatcac tgatccttat ccacagactc ctcatttgga
     9841 atcagcaatc tttctaaaaa gatatgaatg aagagacgat tttctttggt tcttaaaatt
     9901 tgtcaaatta tgtataaaat caattattct ctaactcaaa atggaatcta caattatgaa
     9961 aacatctcaa tcccttttaa cgtctactcg attacaaaaa atccttttgt ggctaatgat
    10021 catttactcc tttgaggctt gttttgtgaa aaactccaca catagcagtt tctgggataa
    10081 tttcttttca aatcaacagt tgctcattga tcgatatgca gatacagatc tttacccaat
    10141 tctctttgga tctttaaagc cagaagaaga aaaacttctc accaagaaag ataacaaact
    10201 atatctatcc ggctatgtta ccaagcaaaa caattccctg atacctaaga ttgacgacaa
    10261 agaaaatcca acagaacaag atgtatattt taccaaagtg gaatacatta aatctgaatt
    10321 taaagaacgc ccaacgtata aaataaaacg taaaacaaat tttcgttatc ctacctatac
    10381 tatgaaagac ttttttcatt ggttagaagt attttccgaa tctgtcaatg atgaagagca
    10441 acttacaata ctagatcgtt ctgtggtata tcaattttta tgttctgaat ttaaatgtca
    10501 aagtgaacta aatacgggaa gtgcaactct ttccttcgcg ttagacgaaa gaatgaaagt
    10561 taaatacaaa actttttata attccctttc tgatactttg agtaaactaa aatttaaaat
    10621 gaaagttttt tctaaatcag aaaaaatagg agagatttat tcagaaggta aaacaatttt
    10681 cgccaaaatt ctacatttaa aaaaaggtca attgcacaat ctcgaaaatt taactctcta
    10741 ctttgaaatt gatctgaatt tttttggtct aagaatccaa gtgcaaaatt tagaatatca
    10801 tatccatata aaatcatcag agacagaaga aattcttacc ggtaaatttc caatctatcc
    10861 aaaatacaaa gtaagtgggc gattatttta tcttcttcca cctggactta taaatatttt
    10921 tataccccaa gatattgagt cttatatggc tgattttttt gatttattga tacgaacagg
    10981 tgattcagaa ggagctaatt tcactactcg aataaaaaaa ttagaaaaca atgaagtgaa
    11041 tgtgaccttt gaatcttttt ctgaaatttt tcaaaaacct ttccgaccac tcgcatcaaa
    11101 tcggaaacaa aaaagagaag gaagagattt ctatcttgat ttgcggaata aaattgtaga
    11161 agacttgagg ccctaatgct aaaggctttg tttttttgct tgcaagtgat aattctcaat
    11221 ataccttaat caatctaatg caagatatac atacgactac taggctcact gctcttgaat
    11281 caaagtctat acagttttct attcgataca aattaatttt aatagtatca ctcattatta
    11341 ttttaggtct ctccgcaatc atctcgcttg tgactatatt ttataaaagc aatagcgagg
    11401 tcttaatcca agaatacaat ctaagcttag ctagacttgc cggtattcaa gttgaatcca
    11461 atttgaaaaa tacaaattat agagcacaag cttttcattc ttccctcatc caaaaaaata
    11521 tttccgaaaa agaaatttta gagaaaacaa atcacttttt tacaaaaaat ccaaaattta
    11581 tttttctcgg aattattcaa aaaacagaaa atgattcttt caaatttgaa aatgaattta
    11641 tgcaagagaa agtgttttca gagaataaaa tagagaagag tctaatgcca attatccatg
    11701 aagttgtaaa ggatggaatt tctgctgcat ttaagggaac aacttctgtt atcaatgcct
    11761 cttctaaatt taactttcca gttctagcgc ttgtcgttcc ttccgaaaca aatccagatc
    11821 aggttatcct attgtatcta gaaccaacag aaataatggg cgcttttaaa tcggcacttc
    11881 aaactgattt gtttgatgta tttatgatca atcaaaaagg agaagtaatc gctcattcag
    11941 aagatacaaa aacagttgcc caggaaaaca aagcagaagt tccaattgtt aagaaaatgc
    12001 tttcgagtaa tgtcgacaat ggatctcaaa aatattttta caatggagaa gagtatctag
    12061 gttctttcca gcttttagat tttagtggtt taggaatcgt atctgccgta gagtcagaca
    12121 aagtttttga agcgatctat caaatccaaa gaagaaatat agtgattacg attattattt
    12181 taacccttag tttcattgta gtttatcttt ttgcaaatac actaacggtt cctcttactg
    12241 atttggtggg agcaagcaaa caaatagaag aaggaattta caaggtagaa atcacaccca
    12301 caacaagaga tgaagtaggg cttcttacaa aatcatttat tcgaatggca aagggactcc
    12361 aggaaagaga aaatataaaa aatacctttg gtaagttcgt aaatcgtgaa atcgcagaga
    12421 aagctctcca cggcgaatta cagttaggcg gagtgaagag aaactgcgcg atcttctttt
    12481 ctgatttgcg aaattttaca ggtatgtctg aaaaaatgca agcagaagaa gtggttgttt
    12541 ttttgaatca gtatttcact gaaatggtgg aatgcattta taaaacaaat ggaattgtag
    12601 ataaattcat tggtgatgct atcatggcac attggggggc tctttacacg gacggcaacg
    12661 atacaggaaa tgcaatagac gctgctctta tgatgcgaga ggctctaatt caatttaaca
    12721 aagacaaatc acatggggat agacctaagt ttcgttttgg ttgtggagtc aatactggag
    12781 aggtcgttgc aggtcaaatt ggctctccta aaaaattaga atacactgta atcggagatg
    12841 cagtgaacct tgcatctaga attgaatata tgaacaaaga gtttggaaca gatatattga
    12901 tttcggaaga ttcttacaag gaagtgcaag gaaaatacaa agttgtcgct atggctccgg
    12961 tagaaatcag aggaaaatca aagccgcaac aaatgtatgc agtcttgggt aagatagacg
    13021 atagcaacgc acctaaagac ttaaaggagt tgcggcaaat cgttggtatc cccttcaagg
    13081 attaaagttg agatttagat taagagatta tttcgttttc ttgatatgca gtcttgtttt
    13141 agctgcctgt tcttattatc tctatttaga ttttaataag aggccagaag atcgaggggc
    13201 agttaaacaa ggatacatta cgtttaaata taaagtcgct caaagaaaat ttccttctag
    13261 aatgatatgg gaagacgtag aacaaatgct tcctatctac aataaagatt ccattcgaac
    13321 tgatttttta tcagaggcaa cagttacatt aaatagtgga atcaagattg aactagaccc
    13381 agacagcatg atcgttttaa acattcatga aaaattggta aatgttgaat tagaaaaagg
    13441 ggcagttcaa gtctctacca caaaaaatct ttcgaatcca aatgaatttg ttttaagtca
    13501 taaaaaaaca aaagtcaatt ttcaagaatc aacaggtcag tttaaaatcc aagagaatgg
    13561 atcgaatcta gaaatcgttt cgagcaaagg gagcattaca ttagaaaccg aaaccaaaaa
    13621 agaaaatatt tttgagaaac aaaaagcaaa tgtaaatcta gattccaact ctgttgaaaa
    13681 agtagattta aagaatttcg agatgcagcc accacataac gttcgttact ttattgaaga
    13741 agatacaaaa gaaatcatct tcatctggaa tgaaaaatca agtcaagata cagagatttc
    13801 tatttcccaa gatagatcct tcaaaaaaga tacctttaga gcaaacgtca aagacaataa
    13861 gtttacaaaa tctttttcag aaggaattta ttattggaaa atcaagtcgc aagcgggaga
    13921 agaaagtgaa actagaaaat ttagaattat aaaaaatcct ccttttttat tgatttcgcc
    13981 tagtcatgaa aaagaaatta aaacaaaaga tggtaattct actgtccact ttatttggac
    14041 aaagcgagaa ttagctctct cctatcattt tgaaatctca aacgatgaaa gttttaaaac
    14101 cattctttat tctaaaaacg tttttaaaaa tgatatctct gtcccactta caatcggaaa
    14161 gtatttctgg agagttcgtt ccttcgattc aattcaaggg gcaaaggtat ttagtagtat
    14221 caatagcttt tccattgata aagaaccaat tacagagaaa tcaactgtat cttctaccga
    14281 ggaagaagta gaacctatcg aggaagtaaa aacagccaaa gaggctaata tagataagcc
    14341 gcaagaatcc aaaactgttg agacaaagac cgcacagcct acaattctta aaaacccgac
    14401 tttgctcttt ccgcgtgcta attcaaccgt cgatatgaac aaaatggatt ccattccgtt
    14461 taaatggcaa cctgttgctg gagccaaatc ctataacatc aaacttgttc aagagtccaa
    14521 tggcgaactc atcctacaaa cagaagtaaa agcggctaat tataatttta cccaactaga
    14581 caaattagat gtaggcagat tcgtctggac aatcgaagct atccccgaac aagattcaat
    14641 cgaccgctcc tcctccactg ccaaattcaa catcacgtta ggcgaccaac ccgccgcccc
    14701 tgaaacgatt tcgaaaggga agaaagagga gtagaaagaa caagtcactc ccaaaatctt
    14761 ttaaaaacct atttaaattt atcaagtagc gtttgcttga ctgtgactac tattgctgta
    14821 ttttatattt aatatctctt taaagttccc tttattaccc taattgcgat ttggcatgtt
    14881 gcacgactcc ctttttcaaa gaaagagcgc atagaataat cacaatctaa atactctttt
    14941 actttcattg aagataattc aatcattgaa ctaatttccc taaccactct actgctctga
    15001 gcaaagtaaa tacttagata aattaaaatt atcccaagtg ctaaactcaa aaacaatata
    15061 gtctgtatag gcatttgcct catctatcca tgaatacatg ggcggatggt aacaaacaaa
    15121 ccaccatcaa catcctatct tctgattctc ctatcaccaa acatccctta aacttagggg
    15181 taaagttccc ttaaacttat cctaatcgta tctccgaaga tatctttatg ggaagtttag
    15241 tcatatcttt aagggatgtt taatgtatcc ttaatagatt ccaataacaa cctgttcaag
    15301 agccgtaatg atgaattgag tgattcttta tgcgtagaca gatcatgacc tgccctacaa
    15361 ttttaaataa taaaaacata aaccaacaaa gcctatcggt gttaatcggc gctaataggt
    15421 ggtaatcttt taaagctgct caaccaaatg acatcaaata cacaagcacg ggagaaagaa
    15481 ttacgataag ccaaatagga agtttccaga attgtagaag agaaaataaa agaacagaaa
    15541 ttgtaaaatc ttttacagag agaatcgttg tgcggtagat tggatcatag aaagcaaaga
    15601 atagaattgc gtatacagag gaattgatac cagacacaat actttggaag ctatctagat
    15661 ttttgatttg ctcccaaaga ggaaagaagc cataaacgag taaaaagcca ggtagaaaaa
    15721 tagcggttac gctgagtaac ccgcagataa cagaatcaag acctacactc gaagcagaac
    15781 ctaaaaaagc agcaacggaa aataaaggtc cgggtagagt ttgggcaaat ccatagccag
    15841 ataaaaacac ttcgttcttc acaaatccat ttgcgaccat ttcttctttt aagagaggaa
    15901 gaactacatg tcctcctcca aacaccaagg cacctgtttt ataaaaccct cctagcaatt
    15961 gaataaaagc aaaggggaag tagttttcta aaatgggaat tagaaatagg atgagtaaaa
    16021 agaaaaggat agtaaaaaag gaagaaagat accaagacca ttggactttt gcattctctc
    16081 tactctcctc tcgcagtaga atttttccta gtagccctcc tacgaggata acaatgattt
    16141 gccctatcgc attctgcata aaacttaaaa agagaagaga aaccaaaaaa ataaaaattc
    16201 tttttttctc atagcaaaac tttccaccca tttgcaaaag agcttgtgcg acaactacaa
    16261 ccgcgactat cttaaaacca tgtaatattt ttcgtgggaa aagttccggg taatggagaa
    16321 tacccaaagc aaaagaaatc atcaaaaggg cagaaggaag cgtaaatcca atccaagcaa
    16381 gtatagaacc gataaaccca gctctcagtc taccaattac cattccgacc tgactacttg
    16441 ccggtcccgg tataaattgg caaagactta caatatcaac gtattccttt tctgtgagcc
    16501 attttttctt atttacaaac tcgtcttgaa aataaccgat atgtgcaatc ggtccaccaa
    16561 acgaaataaa accaagtcga agaaatacaa atagcacttc taaggcttta ttcattgaat
    16621 tctatttttt tagtttaggc aatacaactt tagcagcaag gctcattaaa aaatcaactg
    16681 gctttggaaa cattttatat gtaaacgcag cagcattgta taaaattcct ggaatcacaa
    16741 ccgattccct ttttacaatc gaatcttcta tacactgtgc aatttcagaa ggatcatcga
    16801 aaggaacaaa gggagtatca tggaactgtt ttgtcatctc agttcgagta gttcctggta
    16861 agacacaacc cacatgaatc tggtattgct ctaactctat ttttaaactg gtgcaaagag
    16921 aatataaagc tgctttcgtt gccgcataac tagaatttcc aggaaatgga actatcgcac
    16981 taaaagaact cacagtaata atatttcctc cacctagact ttttagtttt ggaatataag
    17041 atttaatgat aaaagcagga gagatatagt ttagattcaa ttctttttgt atattttcaa
    17101 tagtgttatc ttcaaaggta acacttgtgg aaatacctgc atttaaaatt aatgtttgaa
    17161 tgtctgggaa tctagattgc aaatgggaaa cgagttcatg tgttttttct atctcagaaa
    17221 gatctgcaac tacatagtca actttcacac ctatcgcatg acattcttct gcaacagact
    17281 taagatctct ttcatttcta gaaactaata ctaagtcgta acctttttgt gcaaagtgaa
    17341 tcgcagtcgc ttttcctatt ccgcgtgatg ctcccgtaat cagtgctttc ttattcatat
    17401 caaatcctat tgattttttt aaatatttgt tggtaaatga gtcaactaac tctttaatgc
    17461 agctatatgt gcttcaacag aatcagccaa agcagaaaga ttataaccac cttctaaaaa
    17521 ggaaatgagt ttacctttgc aatgctcttc tgctttcttg cggataatat ctgtaaacct
    17581 ttcatataca gaagatgtta aattcattcc acctaatgga tcatctttgt gagcatcaaa
    17641 gcccgctgag actaatataa aatctggttg aaatcgatca atcgccggaa ctatcttttc
    17701 agtaaaagca tttagataat cttcattgga agagcctctg ctcagtggta tatttaaatt
    17761 aaaccctttc cccttccctg ttcctatgtc tgtttcggca ccactacctg gataaaatgg
    17821 atattgatga gtcgaagcaa agaaaacagt atcatctgcg tagaaactat tttctgttcc
    17881 atttccgtga tgaacatccc aatctaaaat aaaaatcttt tgaatcccct tagattgtaa
    17941 atacttagcg gttatcgcaa tgttattaaa catacaaaat cccattgcat gatacttttc
    18001 cgcatgatgt ccgggaggcc tcacaagcgc aataccgtta tccgcctctc ctctcaaaat
    18061 aacatctgca agtgcaatgc ccgatccact cgcaagcgtt gctgctttaa atgatcctga
    18121 tgaataagga gtatctccat caaagtagcc cactccttgc ttgactctct tttgaaagtc
    18181 ttttatataa gcagaagaat gtatggttgc aatttcgtct tctgtggctt ctctaatttc
    18241 tggttgtatt aattccgaat aataagaagt agattttatc ttattaataa ttgaaacgag
    18301 cctttgatag ttttcgggat gctgttgtcc tgtttcatga tctaaaaatt cttctctata
    18361 ggcgagtgca gttttcatta tctagatttc tcatttaaaa aaatatactt agtccagtta
    18421 taaatccaag taaagcccag aataacaacc acaacatttg catcagattt taaataaaac
    18481 gttaagcccc agaaaaaagg aagaagcata aatagaaagt atgcactcag taattggatg
    18541 gagctgacct tcttccaatc gagaatagaa tttctaagag cagatgttat agaaaaatag
    18601 gtataagaaa agcctaatga ttttttagaa agatgcaaaa acagaataaa cagaagtgta
    18661 attaggattg aataaatcat gttggagttt ttttatttta aatttcgaac ccatctcttt
    18721 tgaaaaacaa aagagatgga tctatctcta aaaattttag agcatgtctt ttacatcgtt
    18781 tctttcagaa actagttcat cgtgtgtaac tttgaatttt tcctttccaa agtcattgag
    18841 aggaactccc tgaacgattt tataatcttt tccgtcagag gaaattggat atccaaagat
    18901 taatcccttt tccactccat agcttccatc ggaacaaacg gcaacactaa accaatctcc
    18961 tgattttgta ggagttgtta agtttacaac tgtgtcaaca actgcgttag ccgctgaagc
    19021 tgcactagaa agacctctag ctgctatgat agctgcacca cgtttttgaa cagtagaaat
    19081 aaaatctcct ttcaaccata cttcgtcttt aattaaatct gttgctggtt ttccgtcaat
    19141 ttttgcattg taaaaatctg gatattgggt ggcagagtga tttccccaaa ttgctaaatt
    19201 agtaacttta gaaacaggtt gtcctgattt ttgagcaagc tgtgtcttcg cacgattctc
    19261 atctaatttt gtaagagcaa accatctgtt agaaggaact ccttttgcat tattcattgc
    19321 aatgaggcag tttgtattac atggatttcc cacaactaat actcttacat cattgtttgc
    19381 ttttgcttca atcgcttttc cttgtttagt aaaaatacca ccattgattt ttaataggtc
    19441 gcctctttcc attcctgctt ttcttggaac agatccaact agtaacgccc agtttacatc
    19501 gttaaacgca acatctaact cagaagtaac tgttactttc tgtaaaagag gaaatgcaca
    19561 atcatccaac tccattacaa ctccctttaa tgcaggaagt gctggttcca attctaatag
    19621 ctgtaactca accgctgtat ctggtccaaa catttgtcca gatgcgattc taaataacag
    19681 ggaatatcca atttgtcctg ctgctcctgt aacagcaact ttaactgttt tactcatatt
    19741 ttctccttat ttacttaact cttcatctat aattttttgg aaagctccaa ttggttgagc
    19801 tccctcaaca aatataccat ttataaaaaa tgcaggagtt ccattaactc caacagactg
    19861 cccatctgca atatcagact gaatctcttc agaaattttt ccagagtcag caagacatgc
    19921 attaaatttt gttttatcta atccggcttt ttcagctagt agtataacgt tctcttttac
    19981 taaattgtct gaattatcaa agagtaagtt aaaatactcc cagtatttat tttgttcaat
    20041 agcacagtta gccgcaatat gtgcaaacat tgcattacgg tgaaatggaa gtggataatc
    20101 tctaaagacc caacgaattt tgtccttgta ttgttctctt aactgtctag tagtctcttg
    20161 acttcgtttg caataaggac attcaaaatc agaaaattca attactgtaa cctttgcttt
    20221 atcaggacca atagaaggat ttcctttctc agcaacgttt tgcttcacct gtttaacttt
    20281 ttccatatga taatctatgc ggtatttttt tgttaattct gcatagtatt cttgttcttt
    20341 taaattcttt aaataaccaa cgattctatc ctgaacttgt tcaagtttag caccaccaaa
    20401 ttgatctttg tattgttcat agattgctgc tatttctgtt tggcttggtg tataggaacc
    20461 tacacctttt gaaacaactt cgttaggctt gatattttgt tctttagctt ctaattctaa
    20521 aattttatca ctcgcaaatt gttcaaatgc acggcttatt ccttccacat aatccttttt
    20581 taatttcata atgacagctg gtttttcttt ttctaaatca gactcagtga atttctttcc
    20641 attgatttcg atataacttt tggatttaaa gtagtcaatc atggcaggga tactaaaagc
    20701 gacataaaga attgtgaaag cggacagagc gataagccct tttttttcat tttccataaa
    20761 ttagaatatt tctcctattt aaccaaaatc actgtaaatc tttaattagc aaatcaaata
    20821 atggctattt tctcaatttt aaaatgtaag cctccacttt tttaaatgct tcaggggatg
    20881 attcgaatga cggattacta cgatatacca caaccttttc ttttttatgc atctgcactg
    20941 caatagaggt tgggtaattt ctttctgctt ctggagcacc ataagcatcc tttaactgaa
    21001 aaaattcatt gtcttcaata aacttaataa gatcatacac ttcttttttt gtgagagtat
    21061 aatccttttc cttaaatgtg gacttagaag gaatgctact aaccggattg tcatacacat
    21121 cttttttctc cgttagaaca aaagatgtgt ccaagatacg aatcgttttt gttggagatt
    21181 tatattcaag taagatttgc ttcctctttt gctttggagc aacttgacct aaaatacttc
    21241 caattaacag aaaatgaaca actaagaaaa tcattgaaaa cttcatttga ttcctcctat
    21301 tttaaaatct ttaaactaaa cactaaaact ttaaaataca atttaagatt tattctaaat
    21361 gctttgaaaa tatcttcaaa acaaatcgat aattgatata gtatttatga attcagattc
    21421 gtaaataatt tatctgaaat tgtatttttc tctataaaaa tagaatattt gtgaagcatt
    21481 cattggaaat gaattgctat ataaaaacgt tatttgattt tgatgaaatg cgatttgctt
    21541 aaatctactc aaggatactc atatgaaaat acctatgccg agtaatattt tttggaattt
    21601 atcattacgt ttagaattat ttactagctt aataccaatt ccaacttttg tctattttat
    21661 tgctgtagta ggtgctttca aaacagagga agatataaaa tctgtcgcca tctcaggatt
    21721 gatctgtgga acctacacag ttgcatgggg tttgctttat agatatttta aactaagaaa
    21781 cattatcaga gacattagtt ttttaaaaga aggtaaaaca atttccgaca agagaaaaga
    21841 aaaaatcaaa ttaaatatac ttcactatcc atatcaagaa ggaagaacga taatcgcaag
    21901 atggttactt ggagccactt ctggcttaat actttttgga ttcctcagtg gacaatggtt
    21961 tcctactata cctacaattt ctctttactt aggattactc tttgtcttac caatctctta
    22021 tgtaatatat ttatttggat ctgaaagtag tctgcgcgga ttattgttat taccgtctat
    22081 cgtgaacatt cctgttccat cagagaagat tttatacatg ggatatttta gaaggatctt
    22141 acttgccatt ctatccgttg ctatggctcc tattacaata tttggatata tcatttatgc
    22201 aatagttgca ggaaatttaa gtttcaaaga tcctatttta catgtgactc ttctttctct
    22261 tcaatgcatc acatctttag gaattgttgc ctatgtaatt gctagatcat taaaagatgg
    22321 tttagctgtt acaaataacg ctcttctaga attgggaaat ggaaattttg atgtatctag
    22381 cggcagaaca tcagcagacg actttggaga acaagctcat ttactagctg taatcatatc
    22441 caagcttaaa aatctttatc aggaaataac agatctaaat ctaaacttag aaaataaagt
    22501 agaagagaga acaagagagt taaatttaag tttaacagaa ataaaaaaat taaaaattca
    22561 acaagatgga gattactttt taacttctct actagcacaa cctctgttta ttaatgcgaa
    22621 taaatctaac gaagtgaaaa ctgattttat tatccgtcag aagaaagaat ttgaattcca
    22681 caacaagaaa gcagatttag gcggagacat ttgtattact ggaaatttaa aatttggcac
    22741 acaggaaaat tattcgagtt tcacaatggc aatgaatgga gatgcaatgg gcaaatccat
    22801 gcaaggagca ggcggctctt taattatggg tgttgttatg aattctataa tggctagatc
    22861 tgcctccaaa aagaaaattc ttaattctaa tccagttaag tggctaactg acgtttacca
    22921 cgaattaaat tccgtatttc tcgcattcaa tggttccatg cttttatccg caacagtgct
    22981 tttaattcag gatgatacag gagaagtttt ttactggaat gcagaacatc cctttactgt
    23041 tgtttataga gataagaagg ctagtttttt agaaaatgga ttagaacttc gaaaactggg
    23101 aatcgagtct gagtatgaat tcagagttca taattatcag ctcctagcgg gtgacgtatt
    23161 aatactagga tccgatggac gagatgatat aattatgaat ataaatgatt ctagagagat
    23221 gaattatgat gagaaattgt ttttaaaatt tgtagaaaaa gcagatggga aattagacca
    23281 attagaaaaa gctctcaaag aatcgggaga aataacagac gatttaagtt tactcagagt
    23341 tggctacaaa gaaaacattc aatatccatc atcagaagaa tttattcaat tacaaagtga
    23401 agcctatgac aaagcatcaa attactataa actaggaaat gtaaaagaag cgcttggaat
    23461 tttactaaaa gcatattccc caaattatta tacaaaaaaa ttcaatcgcc tacttggagt
    23521 tctaagttat caaaacaaag attatgcaac ggcggtagac gttttatcta aatacgtaaa
    23581 agaaaaacct gacagcattg atattctcta ttatctatcg attgctttta ggaaattagg
    23641 aaactatgat aagtcgattg aaattggtaa tcaactcttt aagttaaagc aaaatcatat
    23701 aaataatttg ataaatcttt gcgacttata ccgattaacc gcacaaaaag aaaaatccat
    23761 ttactttcta gaacttgcaa acgaaataga ttcaatgaat ccaaatgtag tgaaattagc
    23821 aaatttaatt cgacagagtt agtccgctaa cctcgtgttt tcaatgattc tagaattttt
    23881 ttaaaattat ttgcttgtta tcctacttac tttactgaat tagtattgtt ggcgtgaaaa
    23941 tatttcgacg gttattaaca tattcattta agtataaata cagattaatt tctggtttaa
    24001 tattatctct actggtatca atattcaatg gagtctcgct gactagtatg attccaatct
    24061 ttgattcaat gggagctagt aaaaattata aatttcaaat ctctattaca aaaagagatt
    24121 tatctgcaat caaaattgtc gaatctggag aaaatttttc tagattagat caaatcgaat
    24181 ataaattagc aaagctaaaa gtccgtttaa atgcctattt caatgaatta cagccagaag
    24241 agattgtgtt cttttttgta attctagtat ttcctattta tcttttaaaa ttactctgcc
    24301 tgacgggtgc aatttatttc atcaactcga cgggatatat ggcaattcga gatctacgtc
    24361 tagagattta caagaaagtt cagtatttac ctctcaatac gtttgtgcaa gaaaaaacag
    24421 gaatactcat gagcaggatc atcaatgatg tagatgttct agctaaaata attagttctg
    24481 atctcaaaga tgcaatcaat gatttttttt atgttgtcac acacttacta ttactcttct
    24541 ttatcagttg gaaaatgttc ttggtcgttt ttatcgtgat acccttgatc atgggaccaa
    24601 tctcagcctt tacagaaaaa attagaaaag caacgaaaaa tcaacaagaa agactctctg
    24661 cacttaacgg tttattacag gaagtgatag ctggaattcg tgtaattcgt gctttctcca
    24721 tggagaaaag agaatcaggt cgtttttacg gaatcaacaa agaattggca gataaaacgt
    24781 ttaaaggaca tttttatcat caagtaggtc catcaattat tgaattattt ggttcagtgc
    24841 tagcggctat ctttcttgcc tttggtgctt acttgattag tcatgaaaat ttttctaagg
    24901 gaatgtttat ggcatttttc ttaaccttaa tttttttaat gagaccatta aaacaactaa
    24961 gtatgatgta caacttaata caaagtggtg tctctgcagg agagagagtg tttgaattag
    25021 tagatgctaa aacagatata gaaaatccac cacatccatt aaagttagga aaactacaaa
    25081 aagaaattca tattaaaaat gtatcctata cttacccggg atcggaaaaa tcttctctta
    25141 agaatattaa tattaaagtg aaatgtggtg aaacgatcgc tttagttgga gcaagtggag
    25201 cgggaaagtc aactctcaaa gatttaattc ctagattaat tgatccgaca gaaggggaag
    25261 tcctattcga cggaatcgac attcgcaaat ttaattttaa agacgtcaga cgaaaaattg
    25321 gaatcgtatc ccaggaagtt tttttattta acgcaagcat ccgtgataat attgcttatg
    25381 gcagcttgaa tgcttccgaa gagaaaataa aaaaagcatg tgacgatgct tgtgctactg
    25441 agtttattaa ctcatttgaa aagggtttag atacagtggt gggcgaacat ggagtcatgc
    25501 tttcaggcgg gcaaagacaa agaatttcca ttgcgcgagc cctactcctc gatccagaaa
    25561 tattgattct agatgaagcc acatctgctt tagatgcgga atcggagaga ttggttcagg
    25621 ctgcgttaga atctttatac aaaaatagaa cgaccattat tattgcacat agactttcca
    25681 caatccagat agctaatact atctattaca tggaagatgg ggaagtgtta gaatccggtt
    25741 ctcataatga tcttatcgca ttagatggaa aatacaaaaa attatacgat gttcaatttg
    25801 aaagggtaag ttaaaaatat gcgcatctta ctttatttac tctacccact ttccctactt
    25861 taccaattct tattttacct agatcgcctt ttaaagaaga agcaggaaat tcctgatgca
    25921 tttacaatta gcgttggaaa tttaacgaca ggcggaacag gcaaaactcc tctaactatc
    25981 tatttagcca acctagtcca ttccattttt ccagaaaaag aagttattgt tttgagtaga
    26041 ggttacaggg gcaaaaaaac aaaagaagga atgctagttg agacctattc aaatcctgcg
    26101 gattcaggag atgaaccact tctcataaaa gcaaagatcc catttgcaaa tgtaatcatt
    26161 ggaagaaata gattcgaatc ctttcaaaaa tttgcactgt cgaaaaaaaa atcttttggc
    26221 ttttcagaac taaatccatt gacagatatc aacggaagat tctctcctag attcagagca
    26281 agaaatagaa aaagattttt tatttcacaa tcagatgatg ggatggaagc cccagtgatc
    26341 caaggagtca aaagagtagt tttacttgat gatggttttc agcatcatgc cataaaaaga
    26401 gatttggatt ttgttttagt agatgcaaac aacgcatttg gaacaggctt tacaatacca
    26461 ttaggaagcc tgcgagaaag gataacagcc gttaaacgag ctcattatat aatttttact
    26521 cgttatgcaa aagaaaatgc cgcaaacgtt gaaattctaa aagtcaaatt tattcgaatt
    26581 aaccctgatt taaaatttta caatctaact tatttaccat ccccattgat caaccacaaa
    26641 agagaaatta tctctctaga atctctaaca acaaaaaaag tattagtgtt ttcggctcta
    26701 ggaaatccat tttcttttga aagtctcatt gagtcctata acccgaaaga aatcaaaaaa
    26761 gttcgatatc ctgaccattt tgcatatgat gaaaaaacta tgaatcaact cttccaagaa
    26821 ttgaaaaact atgacattct tgtttgcaca gagaaggatt atattaaaat tcattttatg
    26881 agattaaaaa atgaggaagt tgataagatt tatgtcttac cagtggatgt ttttattaac
    26941 aagcaaagcg aactaaaatc tgacctccaa agattgatat cttctaagaa tgattgacga
    27001 aagaagaccc aagtacaaga tggcactgtt ggtttaaaaa acgggatgta gcgcagcggt
    27061 agcgcatctg ccttgggagc agagggtcgc tggttcaatc ccagtcatcc cgaaaattta
    27121 gacccggtag ctcagctgga tagagcatct gccttctaag cagagggtcg ggggttcgag
    27181 tccctctcgg gtcacaacag acccaataaa aacggagaat gtagctcagt tggtagagct
    27241 ccagtttgtg gtactggccg tcgcgggttc gatccccgtc gttctcccaa ttatcttcct
    27301 aataatttct tagcaagttc tttgtgtctt tgattttcct gcgcaaattt tctggttggc
    27361 tcagcttcta aaatccattc taagtgataa tgatattttt tatgatcacc taacgcttta
    27421 taaacatgtg caatgtataa atgattcaga taaaaatcag gtccataaat cagtgctttt
    27481 tctaaatagg aggcagcctt tttattatct cctttcgata taggccagct aggtatttta
    27541 tggaagaacc agccaatcga tcgaagtggc gctccataaa aataagattc atctaatcta
    27601 agagagtgag taaaacaatg ttctatcgga tctaatagaa aaaaactact catgattcct
    27661 cgttcttctc ctaaaagacc gtaacatact ccaagccaaa atgttgattc gatattatct
    27721 ttatctatcg ctacaccttt ttttccaaat tcgatacctt tttcatagat agcaatttta
    27781 tcttttggta acgctgtttc tcctttccag tattgaatct cagccaacaa tccatacgca
    27841 cgtgcagaag gatcttcaat gattctatct aaaagaattt tttcggataa ctccaaattc
    27901 ccaattccca attctctttc cactataaga cggtgtgctt cttctaagga catacacaca
    27961 aaataaaaaa gttttcgttc tatgtaaaat gaaaaaatta tgtttgtggc aaaggatttg
    28021 ttagttctgt aaacaaaaga gacatgtatt gtttacccga ttttcgaccg tatcgcttcc
    28081 aacctactac tcgtaaaacc ttaccatcaa tgttgacatg cccctcatag tctggcaatc
    28141 tatcactcgg tttattctga actttaaaaa cagcacctgt attgggagta agttcgaaat
    28201 tcatatcatt ctccttgtac taaatcactt caaaaaaaag ccagattctt aagtaagatc
    28261 taaaaataga tgctttttcg cacttgcgat gtatcttctt aagaatccgg ctgacctaaa
    28321 cctattgaga tgcttaacat cgagaaagct ggattaactt tcaatgacta tacttatgta
    28381 tagtaagtat aaatatacat atatttcttc agctgtcaaa ctttttttac agaattttca
    28441 acttctttta aaaaaagttt cttattatag ttaaaactaa ataaaataat tcaatgtgat
    28501 tttgcctcaa attcaattca ccaatcactc atatatacgt tggttataga gctaggaatg
    28561 catttcaata cgcaagaggt ttggaaaaaa aagagtgcgg ataaaggatt ctatcagcaa
    28621 ttttagtcag taaaaataga atgaaaggac ataatgatca gaaaaagaaa cgtagttgct
    28681 ttttgtaaaa aaatttttgc caaaaaacaa tatcatttca taaaaaggaa tgtcaatttt
    28741 acaagaattt ttagtctgtt cctgtatatc cttatttgaa agaattatta tttttcagag
    28801 gaagatatat acaagagtca aagatctttc tctatttcaa atacaaataa gtaagttttt
    28861 tgataattgg cataacgaaa aaaaaaagct aaaggagaaa aaaatgaaag taaataacct
    28921 ttttttagta tatctactct tcagtctttt acttgtttct tgtgataaaa aagataaaaa
    28981 gccattactg atcccttttc aaaacataag tgtgcatggg ggacagacat cacaaggaaa
    29041 tccaagtgat ggacgcgaat ctgttggttc taatggaagt caaaattctg aatcacatga
    29101 aaatgaatca agtaattcaa gtggaggttc ctcaaaaagt tgttccttag acaatccagg
    29161 acacagctgg ggcgatccgc atctcaaaac tctcgatggt ctagtgtatg actttcaagg
    29221 tttaggagaa tactggctca tccgaacaga agatgggtct ggaattcaag ttcgacaaga
    29281 accatggaaa tcaaataaaa aagttacagt aaacacagca gttgctgcaa aactcggttc
    29341 aaaccttgta tccatttact taaatggaaa agtatatgta aatagaacac ttaccacaat
    29401 cgcatctggt agtaagattg agcttggtgg cggttctgtt gctttatcag gtaaaacatt
    29461 taaaattaca ttaccggaag gaactgtatt tacagtagca ttgaatactt cctatattaa
    29521 cgtctatctt accactgtaa ggttaaatgg gaaagtatct ggtctattag ggaactgcaa
    29581 tggcaacttc aaagatgatc taactccaag agatggatca aatgttatct ctcccatttc
    29641 tttcaataat ctttacctag tattcggtga tagttggaga ctcaaaaacg gagaaagttg
    29701 gtttgagtat ggatctggtg aatctttcgg ttcatttgat ggaattccag aaaaaattac
    29761 tacaatcgat ccggctgcaa ttgaccaagc cgagttagtg tgccaagcgc aaggaattac
    29821 aaacaaagtt gttttcgatg catgtgtttt agacgttgcc ctaactggtg atccaagctt
    29881 tgcacaagca atcgatgagg cgattggaag tcctgcagca ggtgcatctt ctctttatta
    29941 tgaaatttta gcagcaatta cagatacatc ttcttctgga aatggtggaa cagatggctc
    30001 tggtggttct acaggtggtg gatcaggctc tgttgacaat ggaggcggat ctggtggaag
    30061 tggaagcggt ggaaattctt gctctaaaga agacgatgac aaagagcatc atggcaaaga
    30121 caaagataag cgcggagata acggaaacca ttatggacgc gatggaaaag agaataaaaa
    30181 aggcaaccga gaagaaagca atgaaaaccg ttttgacaac catgatggaa agaaaaatgg
    30241 aagagatcgc gacgatgaaa gagaaaacaa cggtggcaaa aataaatgct caaaagataa
    30301 tgatgattcc gatgacagtg tttctggacg ctgttcaaaa aattcaaatg cctctattaa
    30361 aaatcatggt agatgttcta tctactggat ttggggacaa aagaaaaaat cttcagacta
    30421 atatctgatt tttaaaaatt tagaaaggct cgagggtaaa atcttgagcc ttttcttttt
    30481 aaatgtctca ccttattgaa ggtgcattaa ttagatttaa cgcgagttcg atataacaaa
    30541 aaatttaaaa gattaccacc gataaagacg gggcaccgat aggtgttgtt attttaagtc
    30601 actggttgag ttatttacgc tctagcctct aacgattcac atattcatcc attaaaccaa
    30661 aatatgaatc catcgctatt tatcgttcag ttatcggtgt gtatcggtgg ttaaatattt
    30721 ttacatcgaa ctcacattag atttaataca cctgcttcat cataaaatgg aatctcaagt
    30781 cgtattgcaa tcgcaacaaa tacttttgtt acaaaattta aactcatcct tatatgatat
    30841 aatttaaagt atatattttc cccaaaaaat atatactata aataaactaa aggtaagcga
    30901 taagccaatc aacaaaacat aaatcgaaag tctttccggc attaagattt tttcttttgg
    30961 taagcttagt ttttttctaa ctaccggaaa aacaaaaatt aaataggaga ttcccccaag
    31021 caaaaaacct gcaacaacat ctaaaacaaa atgttgctta acagttaacg ttgaaagaca
    31081 aatacaaaag gaaaatagat ttgcgataaa accaatccgt ttcgaccaag gtaagacaac
    31141 aatagaagat aaaaatgcat tgctaacatg catactcgga aaaagtgctg tcggcttatc
    31201 aattgtataa tttaaaagga cccaccattc gaaaaaattc ttataatccg taagcacggg
    31261 tctctcaatc acaacaggat aaattaaaaa agtaaaaaaa gatattgcga ttccaaaccc
    31321 atacgcttta gaaccagcta aaaacaattc aaagtcccga atcataaaga ttggaaaaaa
    31381 aattaaaata taaatccaat aataaataga gattgtccaa ggcaaaaaag ggatttgatc
    31441 atcaatactc agttttagat ttgctggact ttctacagaa tagaaagcat ttatcaatag
    31501 atagccaggc aaagcaatca agaataaaac gagtgccatg atggtatttc ttagaattgg
    31561 aggtttctct tttagtttta aaactacgtt tgacagcatt cgaactcctc tattcttaac
    31621 tgatggttcc gaagtattcg atctctcttt ctagtttgat tccacttgtt ccaaaaactt
    31681 tttcctgaat tgtatttact aggtaggata cgtcagccgc tgtagcacct cctatattta
    31741 caataaaatt gcaatgctct ggggatattt gagctcctcc tttcacaagt ccgcgtaaac
    31801 cagctctatc tatcaattgc caacttttaa ttggatttcc attttcgtct tttaagtttg
    31861 gatttttgaa aacggagcca gcgctttttt tattttcagg ttgagaggag tttcttcgat
    31921 ctcttttgtc tttcaaggat gcttcaattt cttctaagtt tccaccttcc acttgaattt
    31981 ggattccaag tatgattgaa tctttgtttt ttaaaaattc agtaaatcta tacccatact
    32041 ctacttcttt ttgggttttt ctttgaatga ctccatcctt taaaaaatca acaaactcta
    32101 ccttattaaa cagttcccca ccatagcagc ctgcattttg aatcacagca ccaccaaccc
    32161 aaccaggaat tgtgctcaaa aactccactc ctgtaaaacc caataaagaa attttcttaa
    32221 aagtaggagt agtattcgca gcagctccaa tataaaactt tccctcttct actttttcaa
    32281 actctttaaa ttcacccgat agacgcaagg taatgaagtc attcggatta tctgaaatta
    32341 ataaattaga cccacctcct aaaattttat atggtagatc taatttcttt agaattaata
    32401 atgcatctag taaactttct tttgtttctg gttcaaccac aaggggagaa attcccccaa
    32461 ttttaaaaga ggataatacg gataagttaa cattggttcg aaaaccaatt ttttttttat
    32521 ttagacattc tataatttct tttttttgcg tttcttctaa agatctcatt ttataaatat
    32581 ataccacata tccaaagtaa gactatctac aaggctattc taattagtca gttcgacagt
    32641 accgaattta atttctaata aaaaagtgta aattcttttg gcactcagta taggatttcg
    32701 tctgtataag cttggtcaat tctttttgtc ttcaaatttt atatgtattg gttctaaatt
    32761 gtttaataaa agattcaagt ataaacagta caaaaactag aaattaccag atttcaaaaa
    32821 tagaactcga caaggacatc ttatattcta atcacctaac gtctatgaat taaggggatg
    32881 tttaaagtgt agattttcta gttgctacag cgaaaatata ttctgcaaga taatgaacag
    32941 ttgtgtaggg gttaatatcg tatagctgca tattttctaa atcgtcttct ttttgtcttt
    33001 ctaaccattt tgcattttcg attgcaactt ttaatttggt ttcttcgata agttcagtaa
    33061 aatcgtagtg aatcttttta ttgtattcag aatcatggtt tgcatgaata taactaacga
    33121 tttcatcaca actcataaat ggtctagctg atttttcgaa atgaagtaaa atccaacgct
    33181 caaaacaaat atttgaaaat gcgatttcta tttttgtgtt ctttttattt tgattccata
    33241 ctctcgtttc ttcgaatgtc tcagaaactc cgaaatgccc atctctgtct aagactatcc
    33301 aaattttttc atacttgagt ttaatgcgct ttagatcatc tattttcttt tttgcttctt
    33361 ttaataaacc tttcggtgaa tgatctttgg gcttataaat ttcaatgtca agaccgacaa
    33421 gatttcttct atacttttca atttttttga agctctggaa ataatgaact tccgtatttt
    33481 ctccttccac taagattaga attttattgt tcattcagga taattttaat tttacgattt
    33541 attgtcagta aactcaaact ctggctcata gataacaggg attccaccga agctaccgtt
    33601 caaataccat ttttcgaaag gaatatctct tcgcactcct ttgatagagg cgagactttt
    33661 aatactagtt tgtcctattt catctttttc agtgataaac atttgatctc ttctaaataa
    33721 ttctccgttc atcaaagagc tatcatgagt agcaaatatt aactgcgcat tatttggatt
    33781 atttttcttg ctatgaaaaa tttgaattag catttttgta agaagaggat gaaggctttt
    33841 gtctaattcg tcgatgatga caattgttcc agtaatgaga ctgccaaata ttgggaggaa
    33901 tattgtgatt aatcgtcttg ttccagatga ttcttcattt aaaaaaagta atgaatttat
    33961 aaaatctgat tctgaattcg cataatcatg cttagtaaaa attaatttat cgtcatttaa
    34021 tgttaatata tcagtaattc cagtgtctgc tgcttttaaa agctcgagta ggtttttttt
    34081 ggctaattgt atttctagta aattatagat agatttcata ttattttcat tttgcatttt
    34141 ttcatataaa tctagatcga ggtaaataaa tattttttta acaatataac tatagacttc
    34201 atttaaaatt tctaatctat agctactact tttagaaagg aaaagttgat tttccaaaag
    34261 cccatcttca atattttttt tattaccttt cagggaatct ccataggtaa tttcctttcc
    34321 atattcgcgc tcaaataatt tagctcgctg accttttggg tagaaatata aagattcgta
    34381 tcgaattttt tcaaaatcta tatcaacttt ataatgatat ttaatttttg aattttcctc
    34441 tttattaaaa agtaaaaaaa ttatttcgaa ttgattcggt atttctgaac ttcctttttg
    34501 aaatgaaaat gggttatgca aattaatttc ctgacccact gaataatttt gtgattcaga
    34561 aactagcgtg actaaattaa aaagcgcttt tagaacatta ctcttgcccg aagcatttct
    34621 accataaata actgccgatt tgagaagatg tagattttta tctacttcaa ttagattatc
    34681 cggaagttca ttttttttac tgatagactt atctgccacc atcgaaagag taactttatc
    34741 tctgatcgag cggaagtttg taaccgaaaa ttcaattaac atagacattt atatccaata
    34801 taaatctaat ttcagttgat ttcccacaaa agcaagcaga atttcgaata aataacccct
    34861 cagttctgac aattctctat ggaatgtaag gtgcttaaat acacccctcc ttattagata
    34921 aaatgtcgaa ttgtcccaca atcatccagc aacatacata gattcaacta aaatatcagg
    34981 aactttcact gaactaaatg catcattgta ttcattggag aatccacgaa tcaaatgaag
    35041 taaatcaaag taatttgaat taagcgttat acgatctatt ggcctgacag gttttccgtt
    35101 tttataaaga ataccctgag cccctatgga tatttctcct gaaatagcag aacatccaga
    35161 agccccttct aattttgtaa tataaagaca ttcaggataa gcagataaaa gttctgtaac
    35221 agtcttactt cctttttcta ctatcaagtt tgaagttcca gtccctgctt ttccggcata
    35281 agaacgactt ccatttccag ttggggcaat ccctgctttt ttagctgatt ctaaattata
    35341 cagaaatgtt tttaaaatcc cattttgaac gataggcgtt accttagtaa ctacaccttc
    35401 actatcaaat agccttgaac caggaaagcc aggaatatgt ggattacaag tcattgtaaa
    35461 ctcatccaca gaaattttac ttcctacttt gtcggctaat ctagattgtc ccttctgaac
    35521 tagttcagca taataaggag acaagaacat tccaataata ccaccactca cgcgattcga
    35581 aaaaacaact ggatattgtt tgctttctac tggctcagct ccaagtagtt caagagctct
    35641 ctcgactgct ttgcttgaca tgaactcagt attaaatata gaaaatgatc gtcctccatt
    35701 cgaataaaca cccatcttac tagattcatt ttctttagcc acaactccta gtccggctga
    35761 aactgcattg ccttttctgg aataattgac ccctttcgaa ttttgaatga tagatctggc
    35821 agaagattta gagactccaa gatagggaac atttacaatt cttttatcgc gactaagagc
    35881 aatttgctct agtttaagtc ctagttcaat catttgatca aacgttatgt gatctacttc
    35941 ttctttgtat gactctaaat caaggtccaa aagtttttca cccattggaa gatctaaatc
    36001 cattttatca gtgatcctgg aatgagcaga tgcatctgtg actgcttgtt taattgcatc
    36061 ttctgaaaat ttttccgtat aagcaatacc aggacgccct tctttgaaaa gtcgaattcc
    36121 aatccctcta gaacttgcga tctctgtgct ttttaccttt ccttcaaaga cttcaacacc
    36181 tgcatcgtct gactctacac ctattacatc aaaactatct gccgtttgac gttttgcttc
    36241 tgcgcacata aattctactg cttcttctaa tttcatttat cttccaccta ctaaaatttc
    36301 atctacttta atacttggtt gtccaactgt tacaggaaca gatccagatg cggcaccgca
    36361 cataccagct gtaatttcta agtcatctcc aaccattgaa atacgaggta gtatttcatg
    36421 accttttcca attaacgtcg cccctcgaac aggagatgta atttttccat ttttgattaa
    36481 atatccttct tctaccgcaa aattaaattc tccagtagcg ggatttacgg atccgcctcc
    36541 catttttttg gcatacagcc cgtcttcaat ggaggatatc atatcttcaa atttatcatt
    36601 tcccttatca atgtatgtat ttctcattct cgaaacagga gcatagtgat aggattctct
    36661 tctagcagat ccagttcgtc taacgcctgt ttccattgac ccgactcggt cagacaaata
    36721 atttttcaaa atcccttttt cgatgagaac tgttttttct acaggatatc cttcatcatc
    36781 cacattgatt gatccccaag agtttggaat tgcaccttca tctattgcag tgacagcggt
    36841 gtgagcaatt tgttgtccaa ttttatcgca gaaaggagat gctttttttc gaattgcctc
    36901 tgtctctaaa ggatgaccac atgcttcgtg gaatataact ccaccaaacc catttcccat
    36961 aacgactggc atttttcttc ccgttgcata accagcggat aacatcagta atgctctcct
    37021 tgcagctgtt tcggaaaatt gaggaacatt gagtgtttca aaaaactcaa atcctctttg
    37081 tgctcctggc gattctccgg ctgaaaacat ttctccacca tcttgagccg taacagaaat
    37141 actaaaacgt gataatactc tgtaatcatt tacctcaagt ccctcgctat taaaaatact
    37201 aacgttcgaa acagaatcaa aggcacgagc actgacttgt gctattttcg gagatatctt
    37261 tctcgctgtt tcatcggcta attttagaat cgataatttt ttatcttgtc ctacttttct
    37321 tggatcgagt gtaattgtat ggatgtttgg aaaaactttt tcttttagat gaatgatttc
    37381 ttttgaattt atatttttta aatcggactt tgttttcgaa agtgcttcga tgagttgtaa
    37441 taaatgctct tcttcatcgt ttgaagtatt tgcataaaaa acctctgttc cataaatcaa
    37501 tcgaattccg attccatagt cggtgccagc actcgctgtt tctactttgc gattgttata
    37561 ggacacagat gatcctcttg tttcttccac gaaaatttct acaaagtctg ctcttttatc
    37621 aagacctgct tgaataattt tttctgcttt tattttgtcc atttctcact ccatagcctt
    37681 tagacgtttt tgaaactaaa gagactagtc tttctgtcga gtgaaaatta tttctttgca
    37741 gcctctttca catcttttcc tgttctaaaa aagagataga ctcgaatgca aaacattaca
    37801 acagacatga ctgattcatc gattggtcct ggaagattgg gaagaatagg atagattaaa
    37861 gcaagtaaga ctggccagtt gttcttaatg ctttgtcgta aatttcgatc tcgaatgatg
    37921 aatattatgg aagttataaa tacaggcaca aacccaagaa tcccggcaat taaatagaca
    37981 aaggaactac cggctaataa caataagccg ccaaatgtat ccggcattcc ctgatcccaa
    38041 ataaaataaa ctccaaaaaa gaaaaataaa taggcgtaaa cagaaagaaa caaaacaccc
    38101 cagataagtc cataaggcat tccagatttt ttggttggtt gaaattcttc ttccattaga
    38161 tgatcccttt ctttttaaaa tcttctatga ctgcttctaa atcctctgga gaatcaacag
    38221 ataagccagc ttggtttgtt aaatagattc caatactgtg acccgcttca agtgctctta
    38281 attgttccag agactcagaa gcctccaaat cactctgcgg caaatcattg taagccaata
    38341 aaaactcttt ttcgtaagaa taaattccta gatgtcgata gacatttact gtttttttaa
    38401 aattggaggg aatcaaagac cttgagaaat aaagtgctct ttgattttta tcgaaaacga
    38461 ctttcactcg atttggatct agttgctctt ctttagaaag gggacaggct gcacttgtca
    38521 tttcccaatt tctatactta agttttaaat tcacaactcc atcaatcaag tctggttcta
    38581 ttccaggttc atctccttga atattcacaa cgatatcgac ttctcttaaa cctttcacca
    38641 cttcaataag ccgatctgtt ccggttggat ggtctggact tgttaaaaca acattaccac
    38701 cagagcgctt aacgcaatta aaaatctctt catggtctgt tgctacaatt acatcagata
    38761 gagtctttgc tttctttgta tggaagtaag tccattcgat cattgtttta cttccaattt
    38821 tcgcaagtgg ctttgccgga agtcttgtgc ttgcataacg tgctggaata ataccgatta
    38881 ctttcataga ttacctaata attccatttg agcgattgag ctaaccaaaa tattttcgtt
    38941 agaatctagg gataataaaa agaacacgac gattatattt caagacaatg cgatttggaa
    39001 ttgtgatgat gccctagccg atggcacgaa tcaacagcaa taatcttaag atcatcaatg
    39061 tttatctatg ccaatcggtg gctatctttt attaatttac aataaactct ctgaagtaaa
    39121 cttctttgat tttaccgttt gtcaaaatat ggttgatatg agctttaatt tcttctctta
    39181 aatccaactg atcatttaga gttctcaaat cttctttggt ctttctagaa ataatcaagt
    39241 taatgatatt ttgcatttgt ggtaatctag tagcaagctc tggacctaac gcttgttgac
    39301 caacatcata tccaaaagta agcttcaact taataaagtg agcctctccc acatctgctg
    39361 tgttgatacg aaattcatct acaaagttaa atgtttctaa tggaggagga gcttttacaa
    39421 gtgcaatatt cttttgttgt ttataaacac taaccgcaga ttgttttgca acaaacatag
    39481 aaataacggc aactaatata acacctgcta taccagctcc aatccaaatt aaccatttta
    39541 taattgggga gggagtcttc ggctccgccg ccggcttacc ttcttcgtca tctaattctg
    39601 ctgctgcatc acccataggt tttctccttt cgtataatta aacttttaat cttcgccttc
    39661 tactgtcgtt tccgtattcg gaagtttagt gttaggcaag ccataattac tttcatttgg
    39721 ctttcttcca actgattttt cagctaaaat tacaatatct attcttctgt tataagcttt
    39781 tgcttctgga ctaccttgat cttctactgc atagggacga gcggatccat agctcactgc
    39841 ttgaaataat cctggagcaa gctttccaac attgatcata tagactgtgg aattgattgc
    39901 ccttgcccct gcaagatccc aattgttaat ataagttctt tcttctctac caacgttagt
    39961 tccaggaaga actgcatctt catcgctatg accttccact ttaacaaatc gatccaaatc
    40021 tcgaatgagt ccagatgctt tttttaaaac atcttttact ggcggaagta aaatagcaga
    40081 tgctggatta aaataatcag ctccaatcaa agagataact aatcctcttt catcctctgt
    40141 gattcttaca cgcccttttt caatttcaga tttaaaaacc tgtgtagctt ggtttctagc
    40201 ttttgaaaga gctttgccgc ttgtttgaga aggcaaactc tctacattca ttcctaattc
    40261 ttccagagaa cctttcgcta atgtttgccc accatcaaaa aatccagtag aggacttgaa
    40321 tacagagagg ataatctgca tttccctctg atttgttttt cctgtcgtat agagcataat
    40381 aaaaaagcat aggagtaacg taaccatgtc tccataagtc aacatatact cagcgacttt
    40441 ttgaatacaa tcaggacatt tttgtttagc catattctaa gattaatcct taccgtcgtc
    40501 tttcaagaca gcacgttctg atggagctaa gtaactagat aatttttctt ttacaatacg
    40561 aggattgtca ccggattgaa tggaaagtgt tccttcaatc atgatttgtt tcatagcaag
    40621 ttcgtcttca ctacgcttag ctaatttctt tacaaatgga attgcaagta agttcgcagc
    40681 aaaagatcca tataacgttg taataagagc cgccgccata ccggttccaa tcgctgaaga
    40741 atctcccgtt cccatgtttt taagcatcgc cacaagaccg ataagagttc caagcatccc
    40801 gaaccctgga gcaagaccgc cgtatgtatc ccaccaagca cgtccagccg catgacgact
    40861 tgcgatattg ctgatttcgg tttccataat atttcgaaca agttctggat ccgttccatc
    40921 cacaaccaat tgaattcctt ttttcaagaa ttcatctggt aattcgttca catcatcttc
    40981 aagtgcaagt agaccttctc ttctggcttt ctctgagaaa gaaacaaggg ttgtaatcag
    41041 tccaggaata tcaaacttat ctataaagaa agcttttcta gttactttcc caatgcctaa
    41101 agtggattcc caacttgccg agatcatagt tcccgccgta gcacccccga acgtaattag
    41161 aacggatgga acgttgataa cgtcaccagc accgagaccg gccattatcg ttccaaaggt
    41221 caataaaaat aatcctaagc caattcctac agttgttgct atgtccatct tatttacttc
    41281 tcctcatttg cggaaatatg tatagcgttt ttgaaaactt ttctttggta ttctatgata
    41341 ctatttataa tttcttctgc tttttccctt acaataatct ttttatcatt ttgcaaagtg
    41401 ataacagaat caggagttga ctcgatactt tctatcatgt tgcaattgat tatgatttct
    41461 gaattattta atctgtgtaa ggttatcaaa ggttatccta ttaagagttt agttctctct
    41521 tatctgtcta tcgacctgat tttttatttc atttatgaat ttttcttctg taaatctatt
    41581 tatattcttt tggaagttcg ctttgctaaa agtgagtttt tcaaaccgat ttattgcatc
    41641 attcaaggag gaaactgtct gctccggaaa aaatacacct gttttttgat cgattactgt
    41701 ttcaagtgca ccaccctgcc caaaggcaat tacaggagtg cagtatcctt gtgactcaac
    41761 tggcgtgata ccaaaatctt ctaatccagg aaaaataaac gctcttgctt ttttgtaata
    41821 ctctacaatt tgctctctag gcagtccacc taaaaattgt atattagagg gaacagattg
    41881 ttttaatttt ttttcatcct gacccccacc aattataaaa agttttcttc cattttctct
    41941 gaatgcttct attgctagat caatcctttt gtatggtgca aaggctgata caattaagta
    42001 aaaatcttcc tttgggctct ctaccacttt gtatccttct ggcaaacagg gtggatgcac
    42061 tacgactgaa tctcttccat aaaatttctg aattctcttt cctataaatt tggaattact
    42121 caagaataaa tcaactctat gagaagaagc tacatcccaa gcgcgcaaat aattagcaat
    42181 cgcctgaatc atataaaatt ttaaaccgct tttagcggga aaataatcat agtataaatc
    42241 ccaaacataa cgcataggag aatgaatata agatatatga atactatcag gggaaggaat
    42301 aatccccttc gcaacacaat gagaagatga aagaattaaa tcaaatcctc taagatctaa
    42361 agtttcaatc gccattggaa acaatggtaa aaaggatctg tatttttttg ctttaagcgg
    42421 caaacgatct gtaaatgctg tatgaatttt acgattctca attctttcgt tgagcgttcc
    42481 cttattgtaa atcaaagtaa ataattctgc atcaggataa atttttaaaa gagagtctaa
    42541 cactaattct ccccctctca ttcctgtgag ccaatcatga ataatcgcaa ctttcattca
    42601 acacttccta gacaaacaaa atcccttcca ttaaaaatga ttcatttaaa atagaaaact
    42661 tatttttata ttcaactaag gacagtttaa aaaagaagtt tacaatttta gaaaactgta
    42721 tagtttgaca ggtgaagttg cagactgagt caaattatgt caaatgcgcc caatgtgaaa
    42781 tataattccg ataatgattt ttattacttt ctagctgtgc tagatgatga gtccgtaaaa
    42841 actactcttc ttcattttaa tctcactgaa atcatcaaca tgagtaacca aatcaaaaaa
    42901 ataaattttg atgagttgga aaaagaagaa atcgtagaat ttttacttac taccttcaat
    42961 gagaaatgtg gaacagaccc agctaagatt ttaaaaattg gacaaagatt tcaaatcaca
    43021 gacaacgcaa tcaacacaga aaagaaaaac tttatcaaca cacttctaaa aaacttaaaa
    43081 agtcgcactc ccaatttcta caaaagcaac attcgtaatt taaaaaagaa aaaagactta
    43141 aatgaagaag atagccgcaa acttggcatg aatgaaatgg caaataaatt aattcatgac
    43201 aataaaaaag aagtcattat cattttaaaa gctctctata cattcaagag attcgaaaat
    43261 atccaactct aagtaatagg ctcatctcga ctaacgctcg acttcgcgcc atgctgtcat
    43321 tttaagggag attctaaaag atgaacccct aaacctcgaa caagcggcaa tggattttgg
    43381 gtggaaagac gatgaacggt tcaactaggc ttggttggtg agctcgtcga atccacaccg
    43441 cgtcgcaccg ataaaagata tgataattta aaattctaaa tcctttctgt atcgttcagt
    43501 tatcagtgcc ttcggttgta atcttttaat tttttgttaa atcgaactga cgttaaatca
    43561 atttccacag ggtatttaaa atctgaacca ttgcataggt atccatttta caataatcaa
    43621 gcaacaaacg gtaaatactt gttagctcct cttcagggag attcatcgtc ttcgcgcgta
    43681 aaaattcaag atttgccaaa tttccatcat tgatttctag ttctttataa tccaatccag
    43741 ttaaagcagg caatacactt tttaaagaag cacttccatt ttgctttgga tgataataat
    43801 aaaagaatcg aaatggatta gataaatcta caaaacgatt tgcaatcgaa gaataccaat
    43861 ctgagtattg tgggaacagt tttacagact cacgtaaaca cttcatttca aagactgcat
    43921 tgtagcaaag aattgtccca tctatggaaa ttatcttaga caaatcatct aaaatttttt
    43981 ctctcggatc aacagagcca tcatcgatat agctaaaatg gttcggaggc aaatccttat
    44041 tatccattac atgcaaagaa taaagaaaag gtatatgttg aaatggtttg ctatttacat
    44101 agactggaat tgccggatta atggtttcaa aatcaagaaa gtaaatagga aattgaattg
    44161 aatttaaaaa ttcggttagt ttatcttttt gtaaaaaagg agaattactt ctttctgatt
    44221 caatctgaac tttttgtgta aatgttaagt cgggatcttc aggaatatct ttgacatagc
    44281 gaacacctaa attaaaaaat tttgtcgtta gatctccacc ttctcgtaaa ttaaaaagat
    44341 ttccttcacc tagatcattc caacaagttt ttaatataca attctttgga gttccacaag
    44401 aatgctcttt actaagatca ggagaatcta aacgagtaat taagctcttt agatagttca
    44461 cttgtttctc aaattgtgat ttggctgagt tcactctatc tgtaacattc ttgagtataa
    44521 aaagctgcga aatatccaat tcacctgtga aagaataatc tggatttaca tgcaaaatgg
    44581 aacaattcga tatttttaaa ccactttgcc ttgcaacatg aacttgaaaa catatatcta
    44641 atatattgtc atgtttgagg ttgattgagg atttaacttc aatgatctcc caagaatcat
    44701 ctatactact tggtatcagt atatttgttc tagaaaaaaa atcagaagtt agaaaagatg
    44761 cgttatacac aatacttctc tctgcaatca gattctgagt catcttatga gacgaactca
    44821 tactatggtt ccgaggaact tcttttccat ttgggtaaat taatttggca tacttaaaaa
    44881 taaattcatt ctgaatattt actctttgaa gattgctttc gttctttgct gaataggtag
    44941 gagttatttc agagtgatat tgatgccata aaagtttgtc gcattttagc ccttgcataa
    45001 agcgagactt tgtcaaaacc aattgattgg atctagaagg cagatttatt tcaggtacat
    45061 ttaatttctg atttagaaaa tctttaaaac gaaaaagttt tagcataatc cgaatttcta
    45121 atctaacaaa ggataggttt ttgaatagca ataaaaaaga tttgacgata cttattttat
    45181 gaatttagtt tttgttctaa tttatttact gcctttgctt ttatttgcag agtcaaaaat
    45241 tcaaaaatct cctaagtcga caaaagtcaa aacaggaatc ctatttcaat ctaataaaaa
    45301 tattcagatc actcttccaa ctgaatctgg attcagaaag gacctatatg atatcaacgg
    45361 aaatttactt gaatcagaat ttatagattc aaatggaaat ttaaaagaag aagaaccgaa
    45421 agtagccatt gagaaaagaa tctacaattc aactggattt cttgaaacaa ttcagacatt
    45481 cgataaagga aaagaacttt cgtctgagac aatttttact tatgataaag cttgtttagt
    45541 cagccaaaaa aattcagagt gtgtaaatga aatttccttc ttagatgaga ataaaaatcc
    45601 cattgaggat gaaaatggta tccataaata taaaacagaa tttgatttaa aatgtatttc
    45661 tttgccaaat cgtaaacctt ccgaatgcaa gactttagaa gcaaatttta acaaaaaaca
    45721 aaagttagta gcggataata atggttatac caaagtcatc agaactttcg atgacagagg
    45781 aaacttaaac ttattagaaa tctacaatat taaaaataaa atcaaacaaa aagccattta
    45841 tgcatatgat tataaatgta tagaaataac aggcaaagcc caagggtgta tcattttcca
    45901 cgaatatgtc aatgaattag aagaaaaaac taaaaataca aattatggta aaaaaatttt
    45961 acagtatgat caaaactgtt taatggaaaa gaaaaatgca gagaaatgca tagctcttga
    46021 ggaaaattat gatttaaatg gaaagttaca agatatagca catcgtttta cttttggttg
    46081 tatggattat tcattagagc ttggagctta tgcaaaaaaa atttccaagt ttgataaaaa
    46141 aggaaatgaa atcgtaagag aattttacaa ttcaaaaaat caattaattg aagattcttc
    46201 cggtggagca aagtatattt ttgcctacaa ccaagaatgt atagataccg gaaataaatc
    46261 tcatctatgt agaacgttaa ccgaagtata tgacaaaaat tctaaactta aatataaggc
    46321 aatctttgat gaaaattgtg tcaaatcggg aaaagcaatc tttgaaactc caaactattg
    46381 cactgcatgg aaggaaattt atgaaaacaa gcaaacagag aataataaaa actctattca
    46441 aattataaaa gcaaattttg acacaaacgg atttaaaata aacctagaac aatttaacat
    46501 caatcataaa ttaagtctga atacaaaata ttattttgat aaagagaaat taatcaaaaa
    46561 agaaaactta gacatcaatg gaaatctaag agaagatgag gatggaattg cgacttacgt
    46621 ctatcaatat ggaaatggaa aatacacaat aaaagaggag actttaggaa aagatggaaa
    46681 attaaaggca aattcagatg gagttgccag aatgatattt agttatgatg aaaactgtct
    46741 caaaaaaaaa tcaaatcgtt atgactgcac tacactagaa gaattccatg acgcaaatga
    46801 aaacttaatc gaagttaagt caaacaaaga ggataattac acatatgcaa aattactcag
    46861 agaatttgat cagtcaggga aacttattct tgaagcatac tatgatttaa aacacaaatt
    46921 gaaaaaaaac tcaaaaggta tatcaaagta taaattccat tatgattcaa atggcgaaat
    46981 gattttagaa gaacaatttg gtaaaaagaa ttctttaaag agaaaaatac agtttgaata
    47041 tgatccaaag tgtaagtcaa tttcgaatga atttacctgt aatacatcca tttcccacct
    47101 aaataaaaga aatgaattaa ttgataagga aaacttggat ggcgttccat atgcaaagga
    47161 aagcagtagt tataaccatg aatgcctcaa aattttatca gagccagaag actgtttaca
    47221 gaaaaaagaa tttaccaatt ctaaaaatca aatcatttat gctgaatact ataaatattt
    47281 taaaatagaa aagagtgaaa ttaaaatgat tcgcttgcaa ttaactcagg acaaaatacc
    47341 atatagtgct gaatttataa aataaaaaaa ggtttcattt ttttattata gggagaataa
    47401 aaggaatatg attgatttta taaagcaact ctttggtatt caatccaagt atgaacctca
    47461 tcctgatgct gtgatcatat cttgtttctt taatccacag aattcatatt atcgccttaa
    47521 ggcatttaga cagttttatg aatcaatcca acatacaaat cattttattc tagaatgcgt
    47581 tattggtaaa gccaatccac aattaccaga tagcaaattt attaaaagag tctataccga
    47641 aaacctactt tggcataaag aatctttact caataaaatc atttctgacc atatagaaaa
    47701 agattttaaa tatattttct gggtagacgc agatgtttta tttacaaatc caaattggat
    47761 tgtagaagga gttgatgaac tcgagaggca taacataata caacctttcg aatattgtat
    47821 tcatttagaa aaaaatgaga caaaacccac ctttaatgta gatgaacaca gaaaaaaaat
    47881 atacgatata aattattcca atcgaagact ttggagaagc tttgctgcga attataaaac
    47941 tgctaatctt aattttgcgc aaaatgaaaa ttacaatatc catggtcatg ttggttttgc
    48001 ttggggtgcg aaaagagaga taattacaaa agtgccgctt tatgatagag cgttaatcgg
    48061 tggagcagat catatcattg ctcatgcggc gactggtcaa attcctcaca gttgtatcac
    48121 aaaatcattt acagacaatc tagaagaagt attaacttgg tctaaaaaat ttttccaaca
    48181 aacaaaaggt aaagtaggct ttgtaaaagg agatctttat catttatggc atggggatat
    48241 agacaagaga cagtatttaa aaagaatcca agactttaca gcaaaaacaa agcagattgt
    48301 ccacaaagat gaaaatggtt tatacataac tcagtctggt gatgacgagt atatgaaaaa
    48361 atattttaaa catagagaag taatgtcaga caatgattat ctgagttcta tgggtataca
    48421 ctatatgacg aattctgata ggaatgatag cactgaggat gcaattattg ctagttcttt
    48481 cttagacaat gagaaagaaa taacaggagg tggaggagag atgggtggag ctggtgcgag
    48541 tggcagttgg gatgataatt cctcatccca tactgcgcaa tcagataact tttcttagcg
    48601 gataatatta atttgcccga tttactgtaa tcgaagtttt attgcatatc tccgttggct
    48661 tcaaagctga atcattccat tcaactacct ttccttcctt cggaataagc tttgtgctta
    48721 cgatagccgt aattacaaag ctactgtttt ttccttggga atcatttcct aaccaaacat
    48781 tcgaattgat attcaaatta ttaattgccc ccgaaccttg tagataattg atcttttttt
    48841 tgtcatttgc attactctga atcaaatgat aaaagaggcc gtccttttgg ctgattgata
    48901 aagtaacttt ttgtttggct ccattcaccg cttcattttc ttttggtgat acaaaactaa
    48961 cagttagatt tttagggcat tcaagagaaa aaatcccaga tgtaaaatta aatgtaaata
    49021 ataatgctaa aattaaaaat tgtttcatta atgatctcct ttttatctag tttatttata
    49081 ttcatttttt aatacaatat aaataaattg ataaattgaa tatcacttgc tatcgagttt
    49141 atcttttgat tctaacatta tctctttttg aataagttta gactgaaaaa acctatccct
    49201 ttaatttccg acattcgaga aattatccaa tcagtttttc aaagaaggga tcgtttcaga
    49261 tttactatta caattttgca ctcgcatacg ggcagcaaag cagttgataa ttgccgcatc
    49321 tcgaatattc ttatcctccg gtctaccact ctgcactagg ggttgattca aattacatcg
    49381 tccaacatcc cctgcccaag ggttagaagt ttctgccaat tcttccctac acttacctcg
    49441 ttcgctcatt actttggtgt cacaatataa gagaagtagt aataatatga atgagatata
    49501 ccgtatttag attcctttcc aattttgttt gtgcctattt ttattaatgt tttctaatga
    49561 ttctaaccaa ttacacaaaa ttagacattt tctgattaga gttactctaa tgatttagat
    49621 cgaaaacatg tgttgtcaat acttatattc aggagattct ttcttaaaaa attacttgaa
    49681 gtaaatcaaa accatcagga ggattcttta aggaaacatt atggctacta tttctggaag
    49741 aatcatcaca cacgaaaacg acattattgg aacaataagc tatgatccca caacaggact
    49801 cattacagac attcaagaag gaataaagga aaatagcaag gtctacgaca ctagctcctg
    49861 tattatcttt cctggatttg gagatattca cattcatgca agagaagatg tgagtggtaa
    49921 acataattat aaagaagatt ttatttctgc ttcgcaagct gccgttaatg gtggagtcat
    49981 tcatgttgca gatatgccaa acaaccctgt tccgcctatc gatgagacta gttatgaaga
    50041 aaaatcgaaa ttaactcaga aatctccgat tcatattaca ctctatgcag gaataggacc
    50101 caatacaaat ccattaaaga agtctgttcc ttataaagta ttcatgggac cttcgattgg
    50161 ggaattgttt tttaaaacca acgaggaatt agaggaagtg ataaaacgtt atgcgggaga
    50221 aaatgtaagc tttcactgtg aagatccaat tgttttagaa gaatcaaaag gtgccgcaac
    50281 tcatgaagat agacgacctt taaaagcaga aattttagca actgactttg cactctattt
    50341 aattgagaaa tacaatctaa caggtaaact ttgtcattat tcgagtggag agggattaca
    50401 gaaaattatt gctgcaaaga aaagaggagt caaagtaacc tgtgaagtga caccgacaca
    50461 tttatttttt gatcgctctg tattgacaga agaaaatcac aaatggtttc aaatgaatcc
    50521 acctctacgt tatgagacag acaaaaatat tttactcgcg gcatttaaag aaggttggat
    50581 tgattatcta gcgacagatc atgcacctca ttctattgaa gaaaaattaa aaggaataag
    50641 cggattatcc caattagata catattccct atttgtaaca catctcattg taaatgaaaa
    50701 agtagattta aaacgaattg cagagacttg ttcccaaaaa ccaggagact ttgtgaaccc
    50761 atatctacca aaggaatatg gaaaaggctt tggttatatt aaaaaagaat attccgcaaa
    50821 cttcacagtt ttaaatttaa aaaaaccaac tatgtttaga aaagaaaata ttaaaagtaa
    50881 atcgggatgg tcgccttttg aaaaccatac atttccagga tcaatagagg cagtttactt
    50941 taaaggaaat cttttagata gaggataaat ttactttaga gaaaatttga aaattaaaaa
    51001 aagatggata ctttaaagtt aattctaatg agaattttaa tttcttggtc atggttttta
    51061 agcctattca ttattctttg gcttagaaaa aagcaatttt ttaaaaagtc agattctgct
    51121 gtatttactc tttggatatc attgtttttt tattcgatat tcgtttttta ttttcattta
    51181 aatactttat taatcccaga taggcatggt catgcttatt caattattgc atttgcattc
    51241 ggcataccat taattctata cttaattata atacctatct ccatttttat tgcaatggtt
    51301 gcttctttta taattgggag atttaaaaat taagaaagtt cggctttgct ttcagaataa
    51361 gcacgaagat tgtcgtagga gtaaattcca gagtcatgat gatcactcca agtgaagttg
    51421 agagcataac gtccgacttt gcgccatgat atgagtttta tctcattgat atgtccagta
    51481 gcggctccaa tcgttccccc atgaccacca caacagactg cacaggggca tttctttcgt
    51541 agatctaaaa gattgtaatg agaaacaaag ccatctttcc aagtaattgt aagagtgtca
    51601 tcactcgtct gaatatcttg gggaaaggtg gctagcattc agtttatttt tttactaaaa
    51661 cggctagtat ctcctgacgt ttgatatctt tttcagattc attcatggag agccattctc
    51721 ttttaatttg ttttttatcg atgcctttca aatcagcata tttctttaat aatttaacag
    51781 ttttttgatt catgtggaca ggattgtaca ctgagcagag aagtcaaata ttttgtttgt
    51841 agtttaaaca tcccaaatta gatatttcat gatatctgaa taaaattata aatatcttta
    51901 gtatataaat tcatcctcta tctttccagt tttcaataaa agttaaaaaa aaagtgttcc
    51961 caataatgaa attatacaca gtcagtctaa tctgttaaaa ggattaaacg ttatgaacga
    52021 aattgataaa tatataaaaa aatttccagc agaggtaggt aaaaaagtag cacatcttag
    52081 ggaaatagtt aaaaaagtag cacccaaagc aaaagaggta tatgaacaca aagtcatcgc
    52141 atttacaata aaagatgatt atttaatttg gtatggcgtg tatcgaaaca gactttgtat
    52201 atttccaaag tctgaaacaa ttgaaaaatt tagatccgaa ttagatggga ttatagtcac
    52261 caaaggaaca atagaatttc aagcatcaaa gccattacct gttgatttaa taactcgtat
    52321 tttaaaatac agactgaaag aattatctaa atcaaatcag gatgaaaaat aaagaaacca
    52381 ttcaacttat ttatttttgc aattcatcgg aaaaatcaaa ttagccgcag actgagctgc
    52441 tttaatttat ttcctaattt caaagaatct ataaataagc cctttaaata gttattttga
    52501 taatagaaat ttctgaattg cataaataaa cctttcaaac aatacagaac tcgaaattac
    52561 ttgattcaca aattaattca ctagacaaac gaattagttt ttaaacatta gaatgcatga
    52621 atccaacaca actagcacta gcctcaaaca atcaacacaa gatatcagaa ttcaaaaaaa
    52681 tattgagtag cggcttctct ctgttaaccc ctaaagaact aggtattgag tttgaagtag
    52741 aggaaactga atctacattt gaaggcaatg ctaaattgaa atccgaacac ctttttaaaa
    52801 tttcgggaat tccatccatt gcagatgact cagggatatg cgttagtgca ttaaatggga
    52861 agccaggtgt atattcagct cgctatggaa aacaaggatt cacggataga gataggacag
    52921 aatttttact ttctgagata aaaggaaatc ctgatcgaaa tgcttattat tactgctgta
    52981 ttgcatttac aaataaggaa ggaactagat ttttttcagg taaatgcgaa ggacttttag
    53041 cggatgacta tgacgaatct ggaaatggtt ttggatacga tccaattttt atctatcctc
    53101 ctctcaataa aagattttct caaatttctg cagaagaaaa aaacaaagtt tctcatagag
    53161 gaattgctct tagagaattt gcaaaatttt taattcaata actcatctat cacgcatgta
    53221 tgagttcaag gaaagttaaa taaatttaat ctttctgctt aacgttactt tttggaaaaa
    53281 taatcttaaa aggttctttt aaaccaaagg taattgctat cgataagatc ctatctaata
    53341 aggactctgt aacttcttgc cacattccgg caactttcac tgcattcttt gtatagatat
    53401 cttccattaa aatcattcca acgctaagac taggtagtga tacctcgatc acatcgccag
    53461 tctgttcaag ataataaagc ttttcgagat aaggtaataa attgggatca actttaccag
    53521 aaagtgtttt tatagaagca atagtcgctt ccggtgaatc atttcgaatc gaaatcattt
    53581 catattccca agctgctcta agaatttttg attctactgg tattgcctct ccggaaactt
    53641 taatatcttc tggcgagcca gaaccgtcat agtttttgaa agataatcta actacatcag
    53701 ccactcgctc aagtcttgga attttagata aaattttata cccatgtaaa acatgctgtt
    53761 cgaatatttt tttttcgtcc cagtttaaca ttctacctgt aatagatttt aatacaaaat
    53821 ctctcggtaa atgaatacaa ccaatcatat agaaatgtgt tgccatatcc atcacccatt
    53881 tatcttcatc taaatccaat tcttcagtaa tgtattttat atgttttttt atacgagcaa
    53941 ttcgactaaa aacttttgag tctaacgttt ctaatatttc tgtaagaata tgaatagatc
    54001 cgattaatgt tttttctaat atttcctttt cttttttctg ataaattctc cgaatgagat
    54061 tattggaaat tctagctttt aaaattgctt tatcaaacgg ttttacaata taatcatctg
    54121 cccctgcttc aagacattca agcacagttt cattttctag aagagtagag atcattataa
    54181 taggaacaga gcgtagtttt ggatttcgcc taattttttg taaggcaata attccatcca
    54241 tgttaggcat gttaatatct agcagtatta aatcaggaac attctcgagt gtagccttga
    54301 gagcttcaaa accatctgcc gcatgaatag attcatgtcc tagacttcta acaattaact
    54361 ctaacgcata tctatactct tcattatcat ctacaattaa taccttacaa ctctcttctg
    54421 tcattctatt gtttcctaat ttactactgg gttaaactac cgtttcttgt aatttttaaa
    54481 tttataactt ttttcattca gatttttttt attccttatt ctattttcat ctttatgcgt
    54541 tcttttttaa ttttatcagc ttgtttcatt agagaaaagc tatcgcatgc tttttaaaca
    54601 tcgaaatttt ttcttctaat ttcaatagat ttacttaaac aattctcaag actaatttaa
    54661 ttgaattaat tcttaattat atttttaaaa tagaaatcta ttttttcttg ctttaaaata
    54721 gaagtaaaaa aaaatctttt gcaaaagaat tcacattctt tgtagaaatt ttcaaaaata
    54781 ttaacaccgt agactacatg aaattaccat tgctaaattc agaaaatata cggggctata
    54841 tttaagaata ttttttattt taaataacca aataaacatt tatctactaa cgttttaaaa
    54901 tgaaatactt tatattcaaa ttcaataaac caaatataat atttataata gagataaaca
    54961 atgattaaag aaaaagtttt agttacagat gatgacattt ccataaggac tcttctgata
    55021 aactacctca ctaaattaaa ttacgaagtt cattccgcag aagatggaaa tgacgcttta
    55081 agtaaaatac aaacacaaca acctccattc aatttattga taactgattt agatatgcca
    55141 ggtatgggag gtaaatcatt aatacaacaa gtaaaagacc tgtatcctga tttgattata
    55201 atggtagttt cttcgcatga tgagagtaaa ataattatag atataatgcg tatgggagta
    55261 tttgattata ttgtaaaacc gatcaatcgg gaagaactgc aactgaaagc aactagagca
    55321 atgtccgcgg ctttattaaa aaaagacaat ttaatttatg aaaaagaaaa aacacttaga
    55381 ttagaaaatc aaatcaattg gtttaattac aaagaacaat caaagtcaaa tgaactattt
    55441 agtcaggaaa aattaaagca aaacttattc acaaatctaa aaacacatct agggcaagga
    55501 atgggatttg gtttactaac gtccgtgagc gaaatgattt tgatgtgtcc taaagacgaa
    55561 aaaggaaact acttaatcag tcctgatata gttgaaattc tagaaattaa tgcaaattat
    55621 gcacgaaatg taatcagtta ttttggagga atcgaaaaaa tctttgatga tggaatccta
    55681 ttacaaaaat tacccatatc tacagtttat cagctaacag agcaaattgt taaaaaacaa
    55741 gctaataatg cagattcaag tcatttaaaa atcattctat cagagggaaa tttttcagat
    55801 aacgtattta tacgaaccaa ttctagctat tatgacgagg taatttccga acttctaatc
    55861 aatgcgataa aatattcaga acaatattct cagatttcaa tcctattttc gttagaatca
    55921 gataaattaa aaatcacagt tttaaataag cctctcaaat atggtaaaaa agaagcagaa
    55981 ggaattaagg aagaatattt aaacttaata tttgaacctt tctttagaat cgaaaaagca
    56041 attacaaatg aatctatgtc cttaaattat ggacttggtt taactaaggt aaaaattatt
    56101 ttagaacgaa tggatgcaac tattcaagta tctaatttca atgattactc tggaaaaagt
    56161 ccatccataa aagttgggtt tacgatctct tttcccatcg tctcttgatt gctattttaa
    56221 tattcttaat ggaacagcat acatgataat tgaaactaag catcatattc aaattttaga
    56281 acctttgaat ccaatcaaaa cgttagtctt tataaatgga tttggcacag agcaagacat
    56341 ttggtctgaa gtaattaaac catttaaaga aaattataaa attattttat ttgaaaatat
    56401 aggctcaagt aaatcagacc taaactattt tagtccaaat aaatacgata gtctcaatgc
    56461 ctattcaaaa gatctaaata ttatttcaaa aaaacttaat ttagaaaatt caatactcgt
    56521 gggacactct gtcggtggga tgataggctt gctatccacc attcaaaatc caaattatta
    56581 ttccaaatta gtaatgatag gctcatctcc tcgctattta aatgattccg attactacgg
    56641 tggattcgaa aaaaaagaat tagagaattt atttgaatct atgagagcaa attattttgc
    56701 ctgggcaagt ggctttgcac aaattactat gaatactcct gaaaatccaa aactatctga
    56761 atactttgca gcttctctaa aatcaattca agcagatgta gcaatttcag tagctaagac
    56821 aattttttat tcagaccata gaaatgattt agcaaaagtc aagattccaa ctctaatttt
    56881 aaaaacacga gaagatatcg cagtccctcc acaagtatca gattacatgc aaagtaaaat
    56941 taccggaagt agaaaaattg aggtcaacgc aaaaggacac tttcctcatt taagttctcc
    57001 aatagaagta attcatgcaa ttgagagttt cttatgaaat attgtaaata taaaatgaaa
    57061 gcttcgcaaa agcattttat tcgagtagag cttggtataa atctagagaa tagacagact
    57121 tatatgcgtt atgcgtaatt atatgaatat agattctata gaaaatttat ttataaaatt
    57181 ctggagagaa ctacttgacc ttggggttga tgacgattca aaactcttag aaaaatacat
    57241 tgaaattttg cataaatatt caaattcgaa gggttcgatt ttttatcaaa gaatagatca
    57301 tataacagtt agaaatatta tttctcatcc aaaagaattt aatatcgatt tagaagaaga
    57361 attcttttat atttttgatt ccaagaataa aattgagatt ttggaagcag aaaatttcgg
    57421 atttactggg aatttatcac tcatcatact tccaatcctt tctggagaaa aggcaagtgc
    57481 atttgtttta ttaatagaga aggaaagtat ttctaaggat ttcttatttt ttctagataa
    57541 tgcaaatgct gctctcgcac aacgtttcat agcaaataga atgctagagg atattgcttt
    57601 aaaaaaacac caatccaata tcatcatgca aacaattcct gaggctgtta tctattttga
    57661 agaaaatgga aactttgtct ggctcaataa tttagccaaa aatttactca aaatagaagg
    57721 agaattttta aaacctgata ttgtatcaag cgcaatgata cgtttacgaa atgcagccat
    57781 taacagtgat gaaatcaatc aagtaggaaa tagaatattc caagaaggaa attcagaaat
    57841 taaaaattgg tattggatct atggtgaacc tgtagattta gtttatcaag tttctgtttt
    57901 taaaacagca tataaaaatt caaatggaca aatttgggtt tttactgata taacgaatcg
    57961 ttatttggca aatgagagat tactagaact caacaaagaa atcaaagaaa aaattatcct
    58021 tgcagaaaat gcaaataatg caaaaagcac tttccttgca aacatgagtc acgagctgag
    58081 aactcctctg aattccattt taggtttttc tcagatactt ctgcaatcga atgagatcaa
    58141 taacaatgat aaagaacata tacgattcat ccaaaaaagc ggggagcatt tactcagttt
    58201 aattaatgat gtattagatt tatctaaaat tgaagcagga aaatttgaat tgcagttctc
    58261 aatcttctct ttacacagct tgatttttac aattggtaaa atgttagaaa ttaaggcgat
    58321 agaaaaggga ttaggatttg aagtaatcat ttctcccaac actccatctt ttgtcatttt
    58381 tgatgaaaga aaactaaaac aagtattgct taacgtttta agtaatgcaa ttaaatacac
    58441 agacaaagga gaagtagttc tctctttgaa tctaatacga caagaaggaa ataaagcaag
    58501 gatttcattt tcaattcaag atacaggaag gggaattgcg aaatcagata tagagtccat
    58561 cttcatgcct tttcagcaga taacaacgaa tagccagtat gtccaaggaa ctggtcttgg
    58621 tctctctatt acaaaatcgc ttgtagaggc aatgggaagt gagataaaag ttgaatctga
    58681 attaggaaaa gggagtcaat ttagttttga aattgaattc gaaataccag aaaataatca
    58741 agaagaggca attagttttg atgaggtaat tggatacaaa ggaaatagaa agaaaattct
    58801 tattgtggat gataacatta tgaatagaat tctatttgag agaatgttga gaggaattgg
    58861 tttcctcata gaacatgggg aatctggaaa agatgcgtta agtaaaatag atatattttt
    58921 tcctgatctc atctttatag atctaaggat gccaggaatt tctggcttgg atctaattaa
    58981 gattattaaa gacaatccaa aatataagga cataaaactg attgcaactt ctgcatctgc
    59041 attcaacgaa acgaggaata aatgcataga agccggtgca gatgatttta ttattaaacc
    59101 aattcaattc agtgaattaa tcactaaaat aacaccttgt ttgaaaattg aatggattat
    59161 tattgaaagc aaacatccac aagtagaaga taataaaaat ataccgctat ctatagataa
    59221 aaaccttcta aaccaattgc ttgattcagc aaagcagggt gaaattgcaa ccatagaaac
    59281 aaatctaaaa tttttaaaag agaaatatcc agatcaaaat cctatttggg aaaaatttga
    59341 aaaatataca aatcactttg aaattaattt attaatagag agcttgaatc agttaatagg
    59401 ggaatagaaa tgagtttaga tgcttttgca ggtgccaaaa ttttaatcgt tgatgatgaa
    59461 ccagctaatc taagggtttt atacaattta ctaaaagact atccattacg tttgtttacc
    59521 ctgcaagata gtaaacttgt aatgaatacc gtggaaaagg aattacctga tataatcctt
    59581 ttagacatta tgatgccagg aattaatgga ttcgaacttt gcaaaatgat taagaaaaaa
    59641 gataaatttt ctggtatatc catcatattt attagtgcgt taaacgacat aaacaatatg
    59701 gttaaaggat tcgagttagg tgctgttgat tatatctcca agccatttca aaatgaagaa
    59761 attttaataa gactcaagaa tcagttaact attcaatttc aaaaaaagga ccttactaaa
    59821 ttggttgaag atttggaaca aaaaaaccaa aaattgatcg aattagattc tatgaagaat
    59881 aaatttttct ctattattgc acatgacgta agaaatcctt ttgctggaat tctaggtcta
    59941 tcagaaatcc tatccagtga ttttgatacc ttaagcaaag aggaaatgaa gaaattagct
    60001 gaaatgataa atagttctac aaataaatgt tataagttat tagaaaattt attaaattgg
    60061 gcaaagtctc aattaaataa tgtagaaata cgtcccatca aaaaaaatct aaagcatcat
    60121 gtagagaaaa ttttttcggt atatcaatta caagcggata ataaaaatat aactttaaat
    60181 tcaagcattc ctgaggatat tttttattac gcggatgaaa atttggtaga tcttgtcatt
    60241 cgaaatttag tgaataatgc aatcaaattc acaaatgaat ctggaagtat agagatctat
    60301 tgtaaaactc agaacgattt attagaaata tcagttaaag acagtggaat cggaatgcct
    60361 ccatctatat cagaaaacct attcaaatta ggaaaaagcg ttactcgaaa aggatcaaac
    60421 aaagaagatg gaacaggact aggattgtta gtctgctatg agtttattca aaaatcagga
    60481 ggaaatattt ctgtaataag tgaagaaggt aaaggtagta tatttacatt tactgttcca
    60541 tgcttcaagt agggttttga aaaatcgata agtagattaa acaggtataa gaaattccca
    60601 acggaagagc aaagcttata ctatttttaa agattataga ttgacacctt tccaaataaa
    60661 tctattctaa ttaaataata agaggtgtaa tcgagctttc attgattaca aattcataca
    60721 atatgataga aatagaaaaa aatccgatta aaaattatcg aaaattaaaa tgtgaagaat
    60781 gtataaatgg aaatatgctg gattttcaat tcagtatggc ttttcaacct attgtagata
    60841 cgagaacaaa aacagtattt tcacaggaag cgttagttcg tggagtaaat aatgaacctg
    60901 caaattttat tttatccaaa ataaatgacg aaaaccgtta cagctttgat caatcttgta
    60961 gagttaaagc aattgaactt gcaagtcgtc tagggctcaa atccaaagtt aatataaatt
    61021 tttttccaaa cgccatctat caaccagaaa cttgcattca gacaactctc gaagcctctc
    61081 agaagtatgg acttccaatc agttctatta ttttagaaat aacagaagga gaacaggttg
    61141 taaatcattc gcatatcgta tccattttta aagaatatcg aaaacaaggt cttctcacag
    61201 caatagatga ttttggtgca ggatattccg gattaaatct acttgctgat tttcagccag
    61261 atttcttaaa gctagacatg cttctcattc gaaatattga taaagataaa acaagaagaa
    61321 tcattgtgaa ttctattgta aaaatttgtc aggaaatcaa tatctctgtg attgctgaag
    61381 gagttgaaac aaaatcagaa ttagattgtt tagaagatct cggaattttt cttttccaag
    61441 gatattatta cagtaagcca agttttgaag ctttaacgac tcctaaagac tttcaataag
    61501 ttataaagga gcgcaagcat ttgcttgtct ctacgatcat atcaattcaa gcataacagt
    61561 tgaaaaaaca gctaatcctt cacttctgcc gagcgctccc attttctcgg tagtcgttgc
    61621 tttaacagaa acttgatcaa ctgggatttc taaaagttta gcaagagaag aaataatgtt
    61681 gattctatgc ggtgcaattt ttggcttttc tccaataatt gtattgtcta catttaacag
    61741 cttgtagcct ctttcccgca ttaattctaa acattttttt aaaataatgg aggaatccat
    61801 gttcttcaaa gatttatctg tatcaggaaa gtattgtcca atgtctccaa gacccaaggc
    61861 tccaagtatc gcatcggata acgcatgtaa aacaatatcc gcatctgaat gcccaatgag
    61921 tgaccattca ctttcaatgg tataaccacc tagaatcagt ggacgatcgg gattcaccgc
    61981 caattggtga aagtcaattc catttccagt tctatacttg gggggcatct agtagctcta
    62041 tatcaggatt attttttttg aagtaattga gttcccaatc tgtttcaaat agaactgcca
    62101 tacgtccatc tctatcaaat atttgatttg tagaagaagg aattctaggt tcatcttcct
    62161 tcttcaccca acgacaggcg ccgtaaggga gagaagttat attactcttt gcattgtatt
    62221 catctagtag tctacgttga aaaacttcaa actgcaactt tccaagcgca cccacaatcg
    62281 ggtaaccact gccaatagtt ctagaagtga ataaatgtaa aatcccttct tctgagagtt
    62341 gctcaattcc ttttttaaaa cttttaaggg acatactatc cggacaagaa acagtcgcaa
    62401 atatttcagg agcaaacaca ggtagtggtt ttaattttgg aataggacca gaggcaagca
    62461 cgtctccaat tttataagtt ccaggattta ctagtccgat gatatctcct ggatatgctt
    62521 cgtctatcgt atttcgatct tgcccaaaga aggcaaacga agatgctagc ttaataggct
    62581 tatccaaacg ttcatggtta acagttgttc ctcgctcaaa ctttccggaa cagattcgaa
    62641 caaaagcgat acgatcccga tgcactttgt tcatgtttgc ttgcacttta aaaacaaaac
    62701 aactaaaagg agaattgatt ggatctagct ctcctccatc tcttagtccg atatgaacag
    62761 gtggaggagc gagttccaaa aagctgtcta gaaataattg aattccaaaa ttattgacag
    62821 cagatccaaa aaatacagga gtaatcactc cacctaaaaa atctttatct gtaaaaggag
    62881 atattccatt ttcaatcaaa tcaatttctt ctctaaattt ttgtaaaata tccgaatcaa
    62941 tttgttcttc gagactaacg tctgttattc cactgagttg aacaggggct tgataagctc
    63001 caccgggagt tttttgaaat aagtttaatt gcttagtctc tctactataa actcctttaa
    63061 agtcaggacc tgtcccaata ggccaaacga ctggaacagc cgttataccc aatacctctt
    63121 ctatctcatc gagtagagca aatagatctt ttgtaggacg atccatctta ttaataaaag
    63181 tgataattgg aatccctcta tccctacaaa ccttgaaaag tttccgagtc tgtgcctcta
    63241 cccctttccc cgcatctaga accatgacag cagtatccgc ggcaatcaaa gtgcgatagg
    63301 tgtcttccga aaaatcttcg tgcccgggtg tatctaataa attcaaaaca aaatttttat
    63361 attcaaattg aagagcggct gacgttatag aaatacctct ctccttctcc attgccatcc
    63421 agtcagatgt agcagatttt ctatcttttc gtgcttttac cgcaccggct aactgaatcg
    63481 ctcctccgta taacaataat ttttcagtga gtgtcgtttt tcccgcatcc ggatgcgcaa
    63541 tgattgcaaa ggttcttctt ctagaggttt cttcttttaa gctgagtgcc atagatacca
    63601 ttttttctga atggctcttt tttccaattc tttacaaact taagattggc tctaagtctt
    63661 ttaaaaacga ttggattttt tatttttaaa ccaatccata tctagatatt catagatttg
    63721 aaggacttaa gcatgaaatt agaactactt tttccctttc atctataaga atctttgaga
    63781 aattagataa gacaagcgaa tgacggatta aacacctgtt actgaataat atcaattcga
    63841 actctgagtt ttttcttttt aaaatgaatg aatttatccg gacgaaaacg actgagttca
    63901 aattcataga cacgattgtg aactaaaaga tttggttcaa atccaatttg taaaattcga
    63961 gttcccttat aggttccaat tccattacaa cattcacaat aaatatttga tcctaaacac
    64021 tcaggacata gaattcgaac cgttagtgga actttcacaa tgattttatt tacagtctct
    64081 tcttttttaa ttaataaatc taaatcatga ttcagtccag taaactttgc tcgatctttt
    64141 cttctatatc cagcacgtag taaaccgcgt ctggctaaga atgtcataga agttgtataa
    64201 attaatcttt cctgtggtaa aataaattgc ttttttagtt tttctgcatt ggatatgagc
    64261 gttaaattct ttaagtatgc tttatcatac tcttctctaa attcaccggt taacacctgg
    64321 tatgctgcaa gcagagtttg aaatttttct ttttgtcctg ttcgcttatt gtcgggatga
    64381 tagatttttg ctaattcgcg atagtatttt ttaattacat caggactact tccaaaagga
    64441 agattcagtg tattgtaata atccggaaat ttcgcaatca tatcattata aatatcggct
    64501 tttaaaatta aagataggtt ctatacattt caaaaaaatt ttcattggga ataattcctc
    64561 tttcgtcttg agaaagttct ggttttagac gctttaaaaa atacatatct acatctcctt
    64621 tattttttgc atgcacctta cctcgatatt cgcatacaaa aaaatcttta atattatcat
    64681 aaacagattt ggaaatatta atctcaccag actgaccact tgattctagt cgacttgcag
    64741 tattgactgc atctccccat atatcatagg caaatttttt ctgccctacc actccagcaa
    64801 ccacaggacc cgaatgaatc cctattctca attcccaaaa ttcgaaacca agagagtctt
    64861 gcaattcctt tatttcattc attaaagatt gtatttctaa actagctaaa caagcatcaa
    64921 tcggatgagt tctattccgt
//
LOCUS       DAOQOS010000013        62804 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_161_length_62804_cov_6.381950, whole genome shotgun sequence.
ACCESSION   DAOQOS010000013 DAOQOS010000000
VERSION     DAOQOS010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 62804)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 62804)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62804
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_161_length_62804_cov_6.381950"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(322..630)
                     /locus_tag="PLF66_04480"
     CDS             complement(322..630)
                     /locus_tag="PLF66_04480"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HMZ63327.1"
                     /translation="MLKLYIGKEFRFQYIKTGSNGIITLNVVNPIKGSMDVMKQEFLD
                     KIKKIQNKYQEQGFILLGIFGSVARGEETEKSDIDILYTIDATFPYKGLKKSVSIGIN
                     "
     gene            945..1847
                     /locus_tag="PLF66_04485"
     CDS             945..1847
                     /locus_tag="PLF66_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011069743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HMZ63328.1"
                     /translation="MFSFDNSQEEFNFNGAHIERNFYELEFARPENKIEIISEIAKKI
                     PWQTPIVDVIEKLNDPTLRLSSRNFIDEIHVERVNFCLKQIADKGHANHYLDLEKGIF
                     LLSTIGDRTASYQKFKETLDKLAYRLNELFRLNDIILTDDVKVHLLSRVLYQEEGFNG
                     NQVYYHEPDNSFVTKVLENKLGIPISLSVVYILVGMRLGLPLYGINLPLHFMLYYESN
                     HYSTFVDPFNGGVLIDKDTCVRFLEANGYKDIPEYFSKASTLTILKRMYNNLINIYKK
                     TGPEEMELLLQKQLGILENKTRFT"
     gene            complement(1840..2376)
                     /locus_tag="PLF66_04490"
     CDS             complement(1840..2376)
                     /locus_tag="PLF66_04490"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HMZ63329.1"
                     /translation="MKEVFFETQRLLFRNWIESDRELLIQMNSDLRVMEFFPRTFTSE
                     ESSEWMSRMQLNIKNNGYGFYSVEEKDSSSWIGFIGINEVTFNSDFTPCMEVGWRLKK
                     EFWGKGYATEGGKACISFALNQLSISDIYSFTAKINQRSENVMKKIGLKKVKEFGHPK
                     IEKDHPLHLHVLYKTNVK"
     gene            2515..8835
                     /locus_tag="PLF66_04495"
     CDS             2515..8835
                     /locus_tag="PLF66_04495"
                     /inference="COORDINATES: protein motif:HMM:NF036531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HMZ63330.1"
                     /translation="MKYSTDLYKSDWDKVAEDESKGLPKSALEKVNTIYKKAKDNNNP
                     SQFIKSVLYKVKYIGQVEEDAFAKAIEELQKETNSSTSYAKPLLHSILGEVYWQYYNN
                     NRYYILQRTKTNQFKQTDLRTWDATKLIEATIAEYTESIKDIETTKKIKIEDFRSILN
                     HDPEYVPTGRNLRPTLYDFLAHRAVDFFSGQEHGIAKPAETFILDNPIYLSDIKEFVK
                     IKIETKDQMAYKYYALQILQDLAKYHMEDNDPAALAEIELKRLSFVYNHAVFENKGSL
                     YLKSLETMAARFSNSPISTDVYHAIATYWHSLAHKYYSQPTDENKWVSKKAYDICESA
                     IKKFPNSDGAQNCKALQSQIKSKSVTLTMEKTNIASKPFLSLINYKNLDTLYFRIYKI
                     NRSKINEIKKEFSEYYNKHKAYKYYDEILISRFAKEDPDKIFSIKLPNDKDYMEHRVE
                     VKIPELDFGQYVLFASTDKAMTLNGQSISYAFTNISNLTYIENIRPNQDLEYYVLNRA
                     TGEVVSEAEVNTYYQIYNNKKSRYEINADRSITTDKSGFFTLKAKKEYKYFYMTLRKG
                     NDFLEPIDDYYNGDFYRQMPLYQNPNYKEGNYKTVTAKLFLDRAIYRPGQIIYYKGIL
                     IESNDKKRSIQTNVPVTVTFYNVNYQQVAQQTVTTNEYGTFSGSFTAPTNVLNGQMSI
                     NTSYNGNVYFSVEDYKRPKFAVEFEKVKGVYRLGDKVKIKGFAKAFSGANIDGAEVKY
                     RVVRNARYPYWWGYSRWFNPSPQMEIKQGVLKTDEKGYFEVEFEAIPDPSVPKEPGIS
                     FSYQVNADITDLNGETRSASGFAYVGYTSLIVNVNVPEMVNLTHESPFEISTMNLNGE
                     FEPSQGYITIHKLKSPAHAYRRKMWQKPDKFIISKNDYVKDFPNEVYDKEDDVSTWEK
                     EKEVLNTPFNTEKEKNLTLKNLSKWLTGRYVLEIKSLDKFGSEAKEIKYFTVFSENTK
                     DPIPTPTYNWKLSIKTVGEPGETARFILGSTESVKILYELELDKKIITREWMSFENSQ
                     KQIEIPIKEEYRGNIYAHFVLVKDNQIFTSSEQITVPYTNKELKLSFETFRNKLQPGE
                     KEEWRIKIQGAKKEKVISEMLATLYDASLDMFRKNYWDFSIYPYNYSSSNWQNRNDFN
                     TENFRQFQRDWNVASYGISKSYDYLNWFGLYLYGFRGYYPYQTRSVRTYRNGRRDKEA
                     EVEYDLDDAPMAEERAAPAPAMAQSAPVATGGVNKKSAMRKESFAADAPGEAGPELKE
                     KAESTSSNTNNQSNSGGNGEISGVKARTNFNETAFFLPHLISDKNGEVTVSFTIPEAL
                     TKWRMMGLAHTKDLKYGFIYNELVTQKELMVVPNAPRFFREGDEMEFTAKITNLSDKK
                     MSGKAQIEFFDAITLKPILHGNILKTEKLQSFESEKGQSTVVKWNLKVPEGLQAISYR
                     VLAKSDNFSDGEEMPLPVLTNRMLVTETLPLPMRGKDPKNFKLEKMISNKSDTLVHHK
                     YTLEFTSNPAWYAVQALPYIMEYPYECAEQLFSRFYANSLASHIANANPKIKKIFESW
                     KLAAVSNKEDNSKALLSNLEKNQELKSLMLEETPWVLDAQDETERKKRVGLLFDLAKM
                     SDELERALTKLMKVQSSNGGFAWFNGMPENRYITQHIVTGLGHLDHLGVKRVRENANA
                     WNMTTKAVQYLDWQFKEDYDRLIMLEKRDNLNMSSQQIWYLQIQYLYARSFFKDVPMP
                     NETKEAFEYYKWQAKTYWTSFLGNKMSTGMIALALNRLGEETPDAFTKALSSHKLKDF
                     KSNKTPEQIVKSLREHSLNSDEMGMYWKQGWGYFWYELPIETQSLMIEVFDEVAKDED
                     AVNDLKTWLLKQKQTQDWKTTKATSEAIYALLLKGEDWLQTDRSVEIQLGSKKINPSE
                     DKDINLEAGTGYFKKAWNGSEISNDMGNITVTPKNNTLKTKNAGVSWGAVYWQYFEQL
                     DKITPADTPLKLEKKLFLQENTKTGPVITPIDNKTKLKPGDLIKVRIELRVDRDMEFV
                     HMKDMRAASFEPTNVISRYKWQDGLGYYESTRDAATNFFFDSLPKGTYVFEYPLRVTH
                     KGNYSNGITTIQCMYAPEFSSHSEGVRVKVGE"
     gene            8839..9036
                     /locus_tag="PLF66_04500"
     CDS             8839..9036
                     /locus_tag="PLF66_04500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63331.1"
                     /translation="MSTEEKSSERKSRRLKIKCPICKSEDVERKQAGSFGGEFYATYH
                     CISCDHSDTVNEQKLEEWFGY"
     gene            9065..9580
                     /locus_tag="PLF66_04505"
     CDS             9065..9580
                     /locus_tag="PLF66_04505"
                     /inference="COORDINATES: protein motif:HMM:NF016041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HMZ63332.1"
                     /translation="MLDFFAGLVYANGLEWFAHKYLLHEEGKKKDSFWRFHWADHHKI
                     VRQNDFKDISYEKSLLEWNPQSKEVLALATAGILHLPIALISPAFVAGVFVSGSYYYY
                     VHKKSHLDPDWGKENLPWHYDHHMGPNQDMNFCVTFPLFDHIMGTRVRYIGTEREKED
                     QIRRKQKLAAI"
     gene            9583..10182
                     /locus_tag="PLF66_04510"
     CDS             9583..10182
                     /locus_tag="PLF66_04510"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HMZ63333.1"
                     /translation="MIRKNKNKTKEKILKVAMDLFYERGFTNTSVQDILDRCKLTKPS
                     FYYYFPSKDQLAIEYLTKQEEVNRDFLITLMDSSKTPEDFAVKWVAVIKKWSKVKKYN
                     GCPFINFLSQIEISNNKFNTKLKEIEVNWTSLYKNYLIEQSEKGLIDKTINFDKAARK
                     MALIYEGSVNLWKMSRDVKYLDFMEEELKELMHSIRNKK"
     gene            10179..10940
                     /locus_tag="PLF66_04515"
     CDS             10179..10940
                     /locus_tag="PLF66_04515"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HMZ63334.1"
                     /translation="MKYTIVGLIFFGLLIISFNYTIVEKTNTDLIYPYRMKLYYTKID
                     GLREGTEVSVKGVDFGLVQEIKKVPIEEVPDQRFLEPGKSHAIELTLIMRSPITLWEN
                     YDVKFKNKTSFSGRSIDIDPGYHQTEESKFFHPTYTKEENPPAHSPSAKYYDDFFVAS
                     HNTLEENRSDIRRTMINLRELSVKLKGEKGTLPQLTNTDLAYNGLLDTTADARIVLKE
                     FRRYQEGIRESDTIFIPFSLVLYRQLVGSIYGNQY"
     gene            complement(11044..12723)
                     /locus_tag="PLF66_04520"
     CDS             complement(11044..12723)
                     /locus_tag="PLF66_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015682787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HMZ63335.1"
                     /translation="MHGSEISLLRDIALGIIFATILSHIARLLKQPLILGYILGGVIL
                     GKEMGFGLVTSEASIELISEIGLILLLFIIGLEINLNQLAKMGKSMFALGVFQFLICV
                     FLSIAFFFLLGYRFVSGNFNLLYIGVALSLSSTLIVVKLLQDKVEISTVSGKLTIGVL
                     VLQDIWAILFMGAQPNLLSPEFDKILLSIGIINVILVFAFLVSRFILKPIYESVANRP
                     ELVVLTSISWCFGLCGVADMAGLSKEMGALIAGMSIAAYPYGTDVISKLIGIRDFFVT
                     LFFVSLGLKVPMPTISMIGMAVVVSLFVVLIRFISIAPIVYTAKRGLRNGFVTSLNLS
                     QISEFSLVIIALGQGYGHVTPDVRSLILNSMILSSVLSTYLILFNHEIAALLMKAFYI
                     FGLSDRKKEHHINIENMHDESKRDIILLGYFRIGQSLVDYIHVISPDLAKRILVVDYN
                     PANKNILHDKGFKWEYADLAHPDTLTHIGIDHASIIICSISDVFLKGITNQRLLEQLK
                     KIAPEAKYIMTVDEMEQKKKLMKRGAYRVVVPGEITGEFLFDLLRETMRRV"
     gene            complement(12723..13625)
                     /locus_tag="PLF66_04525"
     CDS             complement(12723..13625)
                     /locus_tag="PLF66_04525"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-like beta-propeller repeat protein"
                     /protein_id="HMZ63336.1"
                     /translation="MKQIIFLLIILNTSKLFSQSGSLPSEFAYNFNCAESKSCNLLLN
                     HTQEDLSHVDGEYKYYGNEQGDFFCEEIYPSKKIKWRRALGAAIYAKPLILDDKVVFG
                     SKDGALYALNKTTGATVWKYNTAGEILTSAAGTKEQIVFSSTDGNIYSISQKYGKKKW
                     LHLANHKMNTNPVVFHDKVFFGDADGMFYCLNADNGSLLWKVHLNGPILPHPTVDKTT
                     LYIATPLDLIAIDLRSGKQNWRVGIPKLAKANVHLSGDNVILYDFVDSITYYSAYSGK
                     KIGTRFQKASGRQKIYNFDGEETN"
     gene            14175..15143
                     /locus_tag="PLF66_04530"
     CDS             14175..15143
                     /locus_tag="PLF66_04530"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HMZ63337.1"
                     /translation="MKKFLSTICLILLVSLHQCNLRFKRIYDPKPIGSIGSQIENKNI
                     TRTKLQFIVIGDWGEKGNFYQKDVASAIEKIMRERKIDFILTTGDNFYPNGVENISDS
                     HFKKSFEEIYSFAQETPWYISLGNHDYYGNIQALIDYQKSNKNWILPSTYYSLIANVA
                     DTDALFLITDTNEFIKIAGFPYRTYIDSNLNQIQIKWMNDTLSQSLYGWKIVAGHHPI
                     FSGGDHGDTEVLKKDFLDIMEANQVDIYFSGHDHDLQYLRHPDYRTHFFISGAGSKVR
                     KLKNNPNTVFAKSEAGFILTTLENKTATIEFVNSSGIEIYKTKIEK"
     gene            complement(15145..16086)
                     /locus_tag="PLF66_04535"
     CDS             complement(15145..16086)
                     /locus_tag="PLF66_04535"
                     /inference="COORDINATES: protein motif:HMM:NF015692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HMZ63338.1"
                     /translation="MNTLEIVKAGIWGVCVADALGLPVQFKSRTYLEKNPVNDMIGYG
                     TFNMPPGSWSDDGSLTLCIAESLSRGYNLDNIAHNFIEWYKNGKFTPFGSAYDVGGST
                     TNSVNRLINGISAYQSGDSKIETNGNGSLMRTLPLALYFYFKNTPDKDQYKIIKEVSA
                     ITHGHSLSVISCLIYIDLALLVLKGFSIEESLNQIKQNKNKYYNYLNQSEQSYFKNIL
                     ENDLKTFSISQIKSSGFVIDSLEASLWCLLNSNNYKDTAIKAVNLGDDTDTIAAIACG
                     IAALHYGYDSIPKTWIQTIVNGKEIDRVCELLANSFQ"
     gene            complement(16094..16624)
                     /locus_tag="PLF66_04540"
     CDS             complement(16094..16624)
                     /locus_tag="PLF66_04540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63339.1"
                     /translation="MLSQIFLFFLSLAIIQNPIHQKKKSCVNPWNLKAKIVESGVKLV
                     EVMSEESSKYTASGVLVNIQEIKILSKSNRVEMKDGYMPYVVFEVSNLPIHLTQIDGF
                     IIKTVHPTMRSKKGVFNSHNADTTITKESGATIAYLEDSFVFQDKFAFEKLPGNWSFQ
                     LYYKECLLAEYKFTTY"
     gene            complement(16635..17474)
                     /locus_tag="PLF66_04545"
     CDS             complement(16635..17474)
                     /locus_tag="PLF66_04545"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HMZ63340.1"
                     /translation="MKKVIWIVLGFLTIALIVLFIKVFPFLKFMNQYEVAEYDKNLTL
                     VMGGGGNSAILIGEKEVLVVESKISSGKDKLYALVKEKAGSKSILLVNTHFHSDHAGG
                     NELYKGNTIIAGAYTREDWIAENGDLGIPTEWIKEKKEIQIGNETVILQNIGQAHTRN
                     DMIVYFKNRKMIFMGDLLMIGLHPFLKEKDGSRIANYLEKQNKILQEYEIEKVIPGHG
                     PIGGKELIQTFQTYFFDTKTAAEGKSDLKQVEEKYKDWVSFLSMASTEATVNYWKKEL
                     AVK"
     gene            complement(17498..18829)
                     /locus_tag="PLF66_04550"
     CDS             complement(17498..18829)
                     /locus_tag="PLF66_04550"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HMZ63341.1"
                     /translation="MFNDIRDVLERRLMAPAVRRILEEEEAYGAYIANRFRYIFLVFI
                     LIGTVINLYTLTDSELFYYGVKIYGIGILIYFLLTLAHSIILAKNVNSLNIFFGYITI
                     IFDYSVLTGMMFAWSQMQSPDNFNYFLKNPGFDYFFLLFILVLIQIRIRLVIFSIILF
                     FTIHFASLGYGLYIGVKLSYDWIDYILGNGIFLSDILIGRPLVFFCIAISVAYSIYRT
                     VYMVRRIAKIESEKVSLSRYFSPMIVEEITKDPDILHTGKRQEVTILFQDIRNFTKMS
                     EKMDPVELVEFLNEFRARMTRVVFANSGTLDKYIGDAIMATFGTPVATPDDPLNAVKA
                     GLQMLASLKEWNLIRKNHGKEEIQIGIGLHSGEVFTGSIGFEGRMEYTVIGDAVNTAS
                     RIESLCKKFQAEFLISEEVYLKVKEQIEVEKLPPVEVKGKEKPLQIYRVIV"
     gene            complement(18833..19576)
                     /locus_tag="PLF66_04555"
     CDS             complement(18833..19576)
                     /locus_tag="PLF66_04555"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HMZ63342.1"
                     /translation="MKGFIVFFLITISLYADLSKAERDNLTKTAEWLALFDIKYSKAW
                     TPPDSNKPVKLDCSGTVQYLYSKALSKTMPRDSYSQFNFFKEKGWLFEPPKTADNKIN
                     TEELKKNLQFGDLLYWQNSYNVPPDRDPGVSHVMIYMGMDETGVMRVAGSNTWGDGLF
                     TKGGGPDIYFFDPNQKIGCVREIEGDKKSKCLKDSAFIGYARFDAQKEIIPDPKTKID
                     KDPDNVFPKEDSPVSQPVVPELDANPAKK"
     gene            complement(19618..20604)
                     /locus_tag="PLF66_04560"
     CDS             complement(19618..20604)
                     /locus_tag="PLF66_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010570419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HMZ63343.1"
                     /translation="MIFILVAMTSGIISLSAKDSILNVSYDPTREFYKAYNELFAKYW
                     KTKTGKEIDVKQSHGGSGKQARSVIDGLEADIVTLALAYDIDSIAEKSKKIPADWQKR
                     LTNSSSPYTSTIVFLVRKGNPKAIKHWSDLIKPGVEIIAPNPKTSGGARWIYLAAWGF
                     ALKANKNDETKAKEFVTALYKNVKVLDSGARGATTTFINRKIGDVLLNWENEAYLSLR
                     EADAKDPVEIITPSVSILAEPPVSIVDANVDKHKTREVSEEYLKYLYSVEAQELAAKN
                     FYRPSLDSVLKKYKSTFKDLKLFTINELGGWKKAQEKHFSDKGLFDQIYSLK"
     gene            20960..21787
                     /gene="cysT"
                     /locus_tag="PLF66_04565"
     CDS             20960..21787
                     /gene="cysT"
                     /locus_tag="PLF66_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002773574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HMZ63344.1"
                     /translation="MFGKKKSILPGFGISLGYTIFYLSIIVLIPIAGLFIRTSELSFN
                     EFWSVITEERVLASYKISFGASFFAAITNLIFGLLIAWVLVRYDFFGKKIFDALIDLP
                     FALPTAVAGITLATIYSKNGWFGRFLEPMGFNIAYTPKGIYVALIFIGLPFIVRSVQP
                     VLEELDSEVEEAAASLGATRLQTFITIILPALFPSLLSGFILAFARGLGEYGSVIFIS
                     GNMPMVSEITPLLILTKLEQYDYAGATALAVSILLMSFFLFFCINVLQWWAANRHKA"
     gene            21791..22621
                     /gene="cysW"
                     /locus_tag="PLF66_04570"
     CDS             21791..22621
                     /gene="cysW"
                     /locus_tag="PLF66_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012388183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysW"
                     /protein_id="HMZ63345.1"
                     /translation="MKRDTRITEGFLTKFVLISIALLFIFIVLIVPLFSVFIYALDKG
                     YEVYIASIQDEEALSAIKLTFLTVLFTVPLNLVFGIAASWAIARFEFTGKSLLTTLID
                     IPFSVSPVISGLIYVLLFGAQGLFGNYLAEHDIKIIFAVPGIIVVTIFVTFPFIAREL
                     IPIMQSIGHEEEEAALILGANGWQMFWHITIPNIKWGILYGVILCNARAMGEFGAVSV
                     VSGHIRGLTNTIPLHVEILYNEYNYVGAFAVASLLTLAALATLVIKEIINYFSHKADR
                     "
     gene            22628..23707
                     /locus_tag="PLF66_04575"
     CDS             22628..23707
                     /locus_tag="PLF66_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000510775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter ATP-binding protein"
                     /protein_id="HMZ63346.1"
                     /translation="MSIEIKNIQKHFGKFHAIKGLNLDIKAGELVALLGPSGCGKTTL
                     LRIIAGLETADSGSILFSGEDTIDKDVGERKVGFVFQHYALFKHMTVFENVAFGLKVR
                     PRKQRPSKQEIKDKVESLLKLVKLELLQDRFPSQLSGGQRQRVALARALAVEPQVLLL
                     DEPFGALDAQVRKELRRWLRKLHDEIHITSVFVTHDQEEALEVADRVVVMNQGQIEQV
                     GTPEEVYTNPATPFVYNFLGNVNLFHGRIENGKLFLGDKEIKHTQETNAKDNKAFIYI
                     RPYHFDIYLTHQSDTIAAARIEHISPAGSNIKVELIDEFSQPVLVEIPMERYRELGIK
                     KGDNVYISPKQTKVFFDGAYQDYSI"
     gene            complement(23933..27235)
                     /gene="carB"
                     /locus_tag="PLF66_04580"
     CDS             complement(23933..27235)
                     /gene="carB"
                     /locus_tag="PLF66_04580"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015678808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HMZ63347.1"
                     /translation="MPQRKDISSVLIIGSGPIVIGQACEFDYSGTQAAKALKEKGLRT
                     ILVNSNPATIMTDPMLADATYIEPLTVSVLTKIIEKEKPDALLPTVGGQTALNLAMSL
                     VKEGILERYKVKLIGAQAEAIKKAEDRDLFKQVMVSIGMNVPKSGLATNMEEARKIKE
                     QVGIPLIIRPAFTLAGTGGGHCTREEDFEEVAMKGLNASPISQILIEESVIGWKEYEL
                     EVMRDNADNVVIICSIENIDPMGVHTGDSITVAPQQTLSDKEYQKMRDMALKIIREIG
                     VETGGSNIQFAVNPKNGEIIVIEMNPRVSRSSALASKATGFPIAKIAALLAIGLSLDE
                     IKNDITRVTPASFEPSIDYVVTKIPRFAFEKFPGADSTLGVQMKAVGESMAIGRTFKE
                     SFQKALRSLENDRFGFGSDGYLKELIEFHRVPDSEKKAFITKAIKVPTDKRIFNLKLA
                     FNAGFTVEEIASITGMEPWFLRQMEDIHKSEAEFIEKGYSVIRKMKTFGFSNRQLAYL
                     SSKKEIESIISSGSVVSKIKSQVNSILAKKEKEIEEYLNQEKTFPVYKRIDTCAGEFE
                     AYTPYFYSSYDTEDESNVTTTKSVMILGGGPNRIGQGIEFDYCCCHASYALQDMGVES
                     IMVNSNPETVSTDYDTSDRLYFEPLSLEDVMNIYRNEKPIGVIVQFGGQTPLKLATEL
                     EKRGVPILGTSPDSIDRAEDRKRFVEVLQKLGLKQPANGMASTREDARRIASEISYPV
                     LVRPSYVLGGRAMAIINDTAGLDKYMDEAEEISEDRPILIDSFLDSAVEVDVDALCDS
                     ETVFIAGIMEHIEEAGVHSGDSACMLPPQNISDKILSEIKAATRALALELKVKGLINI
                     QFAIKDETLYILEVNPRASRTVPFVSKAIGIPIVKYATMIMLGKKLKELIPQEEYSVK
                     LVNVKEAVLPFNKFPGVDTILGPEMRSTGEVMGIASTAGEAYLKAQIMAGEETPKGGT
                     IFVSINDKTKPELLPYIVKLSKLGYNLLATKGTHKLLSDNGVISSVINKLHEGFPNVV
                     DFIKEKKINLIINTPLSRVTRDEAFAIRQAAIKYRVPCLTTAEAAKALVDGLLEMGNK
                     GFTIRSLQEIHK"
     gene            complement(27469..28260)
                     /locus_tag="PLF66_04585"
     CDS             complement(27469..28260)
                     /locus_tag="PLF66_04585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63348.1"
                     /translation="MRFNLVILFFFFIGKSLLAVDHNWGNKYKVQVKPISNFVSEVKI
                     FDTEKNKLLYESLMAIKHVLKTKSKKIDGKDFFFLQSTVGGEGLISSNLYYFFIDEGV
                     VKQGSIEDIIEFKLMNLDNDKNKELVLTDFKYYKFRIQNCYNALKPDPQYTGKLFPRI
                     FKFHTNKFTEEKKNPELLKKYLSKLEKKIKKKKKITNLYWLSHYFSVAKFIHLDSHAL
                     TFIKQNNSNFTYSCENTKQTNPNGRSFSLKTDFYSFFKKYGREIE"
     gene            complement(28257..28928)
                     /locus_tag="PLF66_04590"
     CDS             complement(28257..28928)
                     /locus_tag="PLF66_04590"
                     /inference="COORDINATES: protein motif:HMM:NF020295.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU N-terminal domain-containing protein"
                     /protein_id="HMZ63349.1"
                     /translation="MKKIFSIFKTIFKEKKTLSVNESPLFFTENAIAKINEHIESRPV
                     GIKSAFKIQIVYEKEKIQCQVGFDDYKLIRKTLYEYPVPVILNEKDELFLRGAYIDYH
                     LENDAFFYYPNIHLEVLSRSKNSILVFYLDRNIVSPKSQIQSIAIQQTSLPNKMPLLI
                     RKLFETDLVESIYAERNFISVEKKVSNQTDLEVEEKITDVILSYYETCGYPLWVSDHN
                     IEAYF"
     gene            29133..29675
                     /locus_tag="PLF66_04595"
     CDS             29133..29675
                     /locus_tag="PLF66_04595"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF015020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HMZ63350.1"
                     /translation="MDNTNKPLLILDLDETLLHANVDLVHNNYSYQTQFHYVYNRPYL
                     KEFLIDTNQSFQLSVWSSGEKDYVEELIEKSFPREIILSFVWDRSHCTHKFDRKLKEQ
                     VYIKNLNKITKEGFSLERTLIVDDSPEKFQLHRKNGISIQPFQGDSNDSELLKLKRYL
                     QKIANSTNFQKLDKVNWNQR"
     gene            complement(29906..31534)
                     /locus_tag="PLF66_04600"
     CDS             complement(29906..31534)
                     /locus_tag="PLF66_04600"
                     /inference="COORDINATES: protein motif:HMM:NF015210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing HNH endonuclease family
                     protein"
                     /protein_id="HMZ63351.1"
                     /translation="MSFENIFKPEAKNLKSLFGNVDSFYEIPDYQRPYSWEKEQIEEL
                     WDDIIDAWSKNEDTYFLGSMILIKKENKFLVIDGQQRLTTLTILFCVLRDLLYPKDRT
                     LLNLIKSQTEDEFRLRLVTQIHRQNHFEKEILKSVDFTRMERTNRKELSNYEFACLVL
                     KEKILASKKSQLDKIITYLYEKVVILTIIAYSEENAVKLFQVLNTRGLDINNYDLYKG
                     YLMEKIAKQKKDQFIQNWINIERISEDTEEHVDNLLTYYAYYKLNNVPKTSIYIALSK
                     NKKLSKEKPEAIVYDFLKFSESYKRIYNSEKNMIHSFFYLPYTSFWKSILTTIVHEKY
                     PDEKIYFLIRKLFYLYWISGVSLSKLKHIVMELILMIKGKKKINEIEKFIHTKLKEDN
                     IKDLLKISLTTDVYDTKWIKPLLILLEYSMHDNNVAFIELGRYFYIEHLLPEKWNRKG
                     LHWSKHWNEEDANNCLNKLGNLTLSLGKKDTIELNRDFDDKKKNYKNLGEFAFSLTRE
                     LLDVRQWKSWDQNHFKKRQELLLGRVRNLFDFDF"
     gene            complement(32597..33376)
                     /locus_tag="PLF66_04605"
     CDS             complement(32597..33376)
                     /locus_tag="PLF66_04605"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HMZ63352.1"
                     /translation="MELRESIIRRRTTNKAFKDQPLREEDIREIIFAANRAPSHFNSQ
                     PWDFILVTDPKVRQEIGDIAGESMQHLISKGVFFKRYLKYFRFSKNEMEEKRSGIFID
                     KIPMVLRPFISKIFGEDGVKLLNFLRVPNILGKDAKKIVTNSPLLLGFMLKKEEYQPG
                     EKSGIYSLISMGAALQNMWLTATSLGIGLQFVSTPMEIPENWDKILKILKVPETHELM
                     AVYRLGYIYEDGKRNTIDWVSDERRSFEDLVSWNYYGGKKV"
     gene            33470..34255
                     /gene="lpxA"
                     /locus_tag="PLF66_04610"
     CDS             33470..34255
                     /gene="lpxA"
                     /locus_tag="PLF66_04610"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015680771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HMZ63353.1"
                     /translation="MKIHPTAIIDPKAELHETVEVGPYSIIEGDVQIGEGSWIESCVR
                     IYSGTRMGKFNKVHHTAVIGGLPQDISFKPETKTYTQIGDYNTFREGFTLHRATKEGK
                     ATIIGNKNYFMGQAHIAHDCVFGDENIIVLNGQVAGHCHIGNHVFISGLVGLHQFCSV
                     GDYAMLAACSKIPRDVPPYVTIDGNPATVIGLNSVGLKRGGFSVETRNKIKHAYKVIY
                     HSKMNTTQALEALKAENDPSPEVQYIIRFIETCERGITDHREI"
     gene            34252..35223
                     /gene="galE"
                     /locus_tag="PLF66_04615"
     CDS             34252..35223
                     /gene="galE"
                     /locus_tag="PLF66_04615"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020776625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HMZ63354.1"
                     /translation="MRLIVTGGAGYIGSHIVCELNARTKHEVVVVDTMEKGSEVNLFP
                     QNEFIKGDIADDAILKKIFSKKVDAVFHFAAWKGAGESMLLPEKYSINNLNGTLKLLT
                     AMSENDCKNFIFSSSAAVYGAPVYLPIDEKHPLNQENYYGFTKYSIEENLRWYDKLKG
                     IKYAALRYFNAAGYDTQGRVRGLERTPANLLPIIMETACGMRPSFEIFGEDYDTPDGT
                     CIRDYIHVSDLALAHLLSLDYILEKKESITINLGSENGYSVKEITSMAEQVVGRPIPH
                     KIGPRRPGDPAKLLASSAKARELLNWKPVFSDSKTLIESMWNVYPKG"
     gene            complement(35474..37264)
                     /locus_tag="PLF66_04620"
     CDS             complement(35474..37264)
                     /locus_tag="PLF66_04620"
                     /inference="COORDINATES: protein motif:HMM:NF013955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HMZ63355.1"
                     /translation="MLYKIFLFLFLPSILFAQIGNTPTETNAPPVTTNNQTTEAPVSS
                     SSKYEIKYFSFKDLSSDQYWVSIVTDRPDELIEELNGKKDKKLRIQIGEIPSDIVIDS
                     AEKDADNPNVINFVVAAYYKIEAGKRRIKLFSKYPNASETQESELEFEIPPLNDQPGK
                     QPYIEKISPEGGTRGDTITVYGNNFGSDIDKVSIVFYESIVSNTGEDTYTELDEKRPF
                     FLSPIVNESQELRFTVPLKKDLMKGGIRKSLKIRMVVAGRPSNFVGLTLLPPTWKFYS
                     ALFGIVVIVLFLIFLYTILRGLIKRQQEREIARKGGEKRPTIVHFFELILTDKATNSY
                     SLSKFQAFVWTIAAIGSYSYVIISQGLLLQNGKIPDFNPNLIILMSISYGGLIAAGGL
                     GAKKPKNEIKSKPPELSNLFCEGGSVDLSRLQLFGFTIVGIGAYIYNLALGNPLQGLP
                     DIPPTLLGLMGVSQTGYLGSKAIGKEITINAVTPNNIEINQENVKVSVLGSGFLTGMK
                     VMVEGFSTPIQAECTSASEITFTMPPFTQTGKHSFTLLPPSGVPIPSNEDINIVEVGQ
                     GGYSMTKSEEEVSPNQEELAAYKDEDQASG"
     gene            complement(37325..38947)
                     /locus_tag="PLF66_04625"
     CDS             complement(37325..38947)
                     /locus_tag="PLF66_04625"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HMZ63356.1"
                     /translation="MSRIRNKLFLNFFPKSILLFFILIPSLFGVDDYFNFLDQSLPEK
                     IEYEKSRKLCVFPFRNKNENEKFQYLSNGIPSVVISNLNGFKFVFDPDVLETVIYHGY
                     GENAKSLPAKESKKTFNRKTISDLNSGQKETLPEKDPRYIKLEVKFIELETPPMLEEN
                     LEIGRKNKCFYVLTGEYSTRENDSMSITLEFTNRKNGKVDQIVETVSIRRAYQEMSSM
                     GFKLKKLLFNKEMATILVETGEEVDALVFIDGHYAGKTPLEKTDVASGQHSISINKEG
                     HETLSRVVNLKKDSVSKYSFQLKKIEKKGLISVESEPSGASVYLGMTYLGETPLQNVV
                     VPLGQNRIRIEKEEYVDYFAGVEVDSSKTTTVNAKLKKGKTEDYYKHRLKVFLDYSYF
                     DLSQFSVYSVGLFYASFMYWDYRARVQRDKIRGDPLQGEVAGGLTLFTTYQSFFSGSN
                     SNSITNLLIFYNYQNTIVQSNERAARQFTTYRTASAGGVFAMLALAGLFYYLGVDNDA
                     FEFAFFPPTPVSPSAIQTNTSYDSYVKYNFRF"
     gene            complement(39178..39888)
                     /locus_tag="PLF66_04630"
     CDS             complement(39178..39888)
                     /locus_tag="PLF66_04630"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HMZ63357.1"
                     /translation="MLFFPAPVKVDLTLSGFPPFENWMHPLGCDQLGRDIYALYAYGT
                     FTTFLISIPARIITLFLSVMVSFLSYVSGRYSNLLLESISSVFLSLPSFLVAIIVLYS
                     LGPELVVFYIAIFLSDWAFAFESIQAKVREVKQSGYVIAARIMGGGNFYLYRTHILPE
                     ISSLLYILFITGIPSVIMTVALFSYMGIDFGTDFLGPGLGEQIAFSKDYFLKSPLALF
                     TPIIAILFLVLSFSKKRK"
     gene            complement(39938..40825)
                     /locus_tag="PLF66_04635"
     CDS             complement(39938..40825)
                     /locus_tag="PLF66_04635"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HMZ63358.1"
                     /translation="MVKEFFQFILFTLCLTVFISIITTIRSQDKTFLYAESSLENSDI
                     QELATERSYLNSFLVSMKSLILLDFGKTESGENVDSHILKKVVPTLQLSILASIGGSI
                     LALFLALFCIYLQFDLLEMFILFLCKLILSTPIFVVSILLFLVFFLYLGVLPPGGYKA
                     NNVLYLILPATALGSRVFARIFYISLEEGKKETSSEYFFFLQTRGFSRSRIVFYYILR
                     KILPLLLIFILIDFSSLLSGSILVEEIFFFPGIGKSLYYAVKSLDENLLRALLFYSGI
                     IFYSLTRFAKLLQYKLALD"
     gene            complement(40840..41295)
                     /locus_tag="PLF66_04640"
     CDS             complement(40840..41295)
                     /locus_tag="PLF66_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016755069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HMZ63359.1"
                     /translation="MNHLLELITPNNIIYDLKAKDKESVIASLVEHGVKIGSIANSAS
                     QEIITSLLNREKSMSTGIGSGVAIPHCSVSLVEDLKVVIGLSKEGIAFDAIDKMPVHI
                     FILLIVPKNKFQEHIKTLALIAKTLNLKEERNKMICSNSYDDILAAFSQ"
     gene            complement(41292..42242)
                     /locus_tag="PLF66_04645"
     CDS             complement(41292..42242)
                     /locus_tag="PLF66_04645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63360.1"
                     /translation="MSSHSILLTNRGFKVFSDDFFVDIFSFLVLYGISIFVALQNLSF
                     AFYLIVLLISSSLFYIFYKLRIGYIYYIGFFYQMLLYLLITPLGFLNWFTLSISLGIY
                     FLFSLKGNLFYKFRFPAGLFLSLLTVSTALFLDHFHILKMNLATSNSEIPMLSFYSGI
                     SGLFLTPMSIPGSSFLNLSFSVLESLDLYLLILVGFVFLRQENFLLDLFLISVFVFLF
                     SFYYRYDILLCKNKIINFSSLWYILFSAPGRSHHLSSFISLIVGVLALAAGWFLIQSG
                     LIFPPILFIIIFFILYGFTFYLVTESIFAKNLLYRYIIRK"
     gene            complement(42229..43404)
                     /locus_tag="PLF66_04650"
     CDS             complement(42229..43404)
                     /locus_tag="PLF66_04650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63361.1"
                     /translation="MATYKTSQFFKIKSSTDLRVPVPSLSELNIQGEFYGRKLTPVGE
                     FPRRLSIGDIIFQDESARILSPANGIAHYYPSTSTIRFTIDGELNFKPQAFNGNYSLE
                     SLKQKIDSTGLVSLDFPGQSLSGLFSSLIPDRESFILFVPFTKENLIDYRSKIFEVYK
                     NELEKLKKNIKEIFPNVNVIDFLTDKKMNYIYPEGSPRYFLHKYVGIDIAKDFPKDRF
                     LYLGPETIYHLIRGLYFNIPFHERYVSVNIINQSGTLEGETKAFLLKNGLNLSDFLNS
                     FQTRYKYFTVNSFYDKQPVYEIGTEFIFDIYKHHSVIICDRIHFEKNEKSCTDCNDCS
                     YYCPVAANPRAILDKDKTGFNSSVCLDCGICSLLCPSHIDLSTRIAEIKQGASFVIP"
     gene            complement(43428..43709)
                     /locus_tag="PLF66_04655"
     CDS             complement(43428..43709)
                     /locus_tag="PLF66_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63362.1"
                     /translation="MSEKVYCANCINCVVVRNFEAEQDKYILRVKCTKKKWSKRSGEE
                     KLYKYFTVARRMQADCEFYEEMGEILPYIKNLKKELPIKDEIYMVKTAS"
     gene            complement(43801..45342)
                     /locus_tag="PLF66_04660"
     CDS             complement(43801..45342)
                     /locus_tag="PLF66_04660"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017215409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HMZ63363.1"
                     /translation="MLIIITALFISIYAVDIFVLFLFSLHTLLMVWLYKKNKSYCESD
                     PNLAFKVTDSNVPFVTVQLPIYNEFYVVDRLIDAAVRLQYPANRLEIQVLDDSTDETV
                     EKVRRIVKEYQAKGIAIEHLHRTNRAGHKAGALEAGFAKAKGEFVAIFDADFVPEPDF
                     LLKTVPYFKDPEIGMVQARWGHINQDYNLLTKAQSYGIDGHFMIEQVARNASNLWMNF
                     NGTAGIWRKSCVIDAGGWEHDTLTEDFDLSYRAELKGWKFRYFKDIVCKAEIPAMISA
                     YKSQQFRWCKGSIQTAVKLLPRIWDANLSWKIKGEAIVHLINYSVHPLMIINILFTAP
                     LLLMEFWSGLNLFDLPVVILYATAAFLSIGSIGPIIFYMYSQREIYPDWKSRIFFLPV
                     MIMIGTGVAVVNTRAWLEAVLGIQSSFKRTPKMRLEKNSDNIKERLKYYVPFDLLTLV
                     EFFMGVYCLFCVYVSIVVEKPFIIGFMFIYSLGFFFVAINSIKEALWSLPSMQEKPAV
                     NVEIA"
     gene            45702..45956
                     /locus_tag="PLF66_04665"
     CDS             45702..45956
                     /locus_tag="PLF66_04665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63364.1"
                     /translation="MEQKKSKFMSLIERITTKAEKLDQLCNIIPQELDYKRHERSKWN
                     SWHSEWKKAWIDSSESYLSDEEREAKRLETSITTRYRTVD"
     gene            46024..46305
                     /locus_tag="PLF66_04670"
     CDS             46024..46305
                     /locus_tag="PLF66_04670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63365.1"
                     /translation="METSLNVRNIIAANLPKENIIPQVDRDLENLKQKEEVNQNEDSF
                     TKNFQKDSKTTLEERLSQVISADEMKNLLSMLMRIPNETTMNSRIDLRV"
     gene            46355..46975
                     /locus_tag="PLF66_04675"
     CDS             46355..46975
                     /locus_tag="PLF66_04675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63366.1"
                     /translation="MGILFIICLIGLGAVFYSQLVQPFIDQRISEIEYPPEIGQKFEY
                     IVNEADRVSYFSIGANTGDIPTKCAAISEDHLNFIFKKDPRNEEYSITIKRSGPVLYK
                     HPRMSHFLKMAGTEKLESHELIGKVAEFRLSDKISKERMIDFIEITLSTAFVISKSGR
                     ERMKFIFTFNKVHPGFNLKTKNRKGLFPLGKDSHHKEDVTPTDEDT"
     gene            complement(46978..48108)
                     /locus_tag="PLF66_04680"
     CDS             complement(46978..48108)
                     /locus_tag="PLF66_04680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63367.1"
                     /translation="MKQTLSFRLRVSIVVFILVLSAFLFFFIIEQSLWLSKIDETNPE
                     VFPRNFFWARLLIASSFSILAGLGAYVFTNNFYESIRHLSHLIQYWSKTLVPNEDRGI
                     SIYPDSEVNQIIQFFNQGILLSKQREEDRLKKAIELNNTSIVERLKPFLPESKLGDLE
                     NLDVSIFPNFTNNPRCDFVTVIDFELGYLCVMAGFEEIEILDSIYKYKLQGLFALSSG
                     LYNAKEEEILYLIWNAIRSTEKENLNLSLVFVSNHSNHISFLQNQKTAMMILSDNGLS
                     TNVSSEIYFDFKRKDTTFSKTILVNPSYLILLSDRVHEILNINPSDLVSMIESEVFSK
                     PTFKNSKELMLRLSLFLDGFGKSQGIGNTLEYLACIIIKKKG"
     gene            complement(48105..48857)
                     /locus_tag="PLF66_04685"
     CDS             complement(48105..48857)
                     /locus_tag="PLF66_04685"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HMZ63368.1"
                     /translation="MKLYTELAEYYYEIEKPGRRFNEEVSFLDEIYRKHKVQTVLDIG
                     CGTGEHVSALQGLGYKIIGTDSSKAMIEQAIKRFPQCKFEVGDLQTYKKEKFFDGIYC
                     IFGTINYLLTNQEIESSLDNIRKSLKPLGLLVLEVWNAVPLRLIQRKPLTTVGHAKVG
                     NMVIKRNRGFKLKKSEGIDDLNETLVEVNFVYNLDKKEIKDKHIMRVFTVSEFSSILK
                     NNKFEIIQIYGNYKMEKYKNHGSRMIVVARRA"
     gene            49037..50332
                     /locus_tag="PLF66_04690"
     CDS             49037..50332
                     /locus_tag="PLF66_04690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63369.1"
                     /translation="MTKYFFLIFFFLILTAVDADTAKRASSQTDVYIWGSIFLALSMG
                     SLLLVVNLFPKGAAYKAYEARLQHLRNNPAAKPKSSNEENAPGQNTEANNLQTEIEKT
                     NPQTKPANATAPVMQSTHTSVKVPETKPSIPAINPVPQQECVIINYNKVFESPSAQPE
                     TSCKDFVNKLSNIIKCKSISIYFVHNQVFSCFIEKKADIFTKMEAGKKSDLSDEILKF
                     LKNKLGAFSSNHADAVLPLVTNNQLFGAVKLEFAESLANLDINPIWSEVKAFAKYFDQ
                     SIHYNLSVQDNETSLFTLEHFNNILNYRVSLDLPQNLSLIKILNTTDKVKTFKDISGC
                     VKEVIGKKPEIYKLSDDLLGIFLTIEDRDKVSKSMGTLVNLLKKNIVNIEIAIGSADY
                     NENIKFANKWHERATNALNSAIQAGKNNFRLFVEPEIKS"
     gene            50336..51394
                     /locus_tag="PLF66_04695"
     CDS             50336..51394
                     /locus_tag="PLF66_04695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63370.1"
                     /translation="MLDYKRILLGLPFLVRSGGVKSSRIVKTRENYFPSKLVPGKKFR
                     FKFFYCDEFIQENRPTILLTHGMSGYGIDDIRMTEAATNLTVCGYTVVVPEFEEIKAL
                     KISQESPRNIKDAFLIILDQKDLNTESIGFFSISFSAGFGLIAFTDPEISTKVKSIIA
                     IGGFAEILDTCNFAMLNYEKDDYPTNILFYNYIHLLYDNCEELAKAFYEAALDNGLSR
                     RGKDQVAPRIYENLSKGHREIFDRVKTSATFRKELGLKISQAYEKEAKSISPIYFIEK
                     IKAPVCLLHGKNDDVISENESIKLSKQMNAYNIDHVLELTGLLSHGDKIPFWKQLSSL
                     PGLSHAFGYFFENLREKK"
     gene            51512..53044
                     /locus_tag="PLF66_04700"
     CDS             51512..53044
                     /locus_tag="PLF66_04700"
                     /inference="COORDINATES: protein motif:HMM:NF015894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FapA family protein"
                     /protein_id="HMZ63371.1"
                     /translation="MEERTQNNMADMVQLSETRLDCISIEIAKDAMSAYLIVEINGVS
                     MRDFSPTMLYNAITNSKLSEEVVNFSLIKEVVDEVTKRKKQVLPINQIQDPNELIVRQ
                     QIATGTPVVPGEDGWVKFYHPHNQRVVIKQDGSADFRNLEKFISIKKGEKIATLFAGV
                     PGKPGKDVYGGVLNAAAIKRPKIIIGANIITTNVSSPEEPEKVFIDYTSACDGVLFST
                     DESVTVSPELRIEQSVGIATGNVKYNGTVNVIGSIEDGAKVECEGSLNVAENLESSDI
                     HVGKDLIVKGGIKAKAKGLVKVVGNVRAKFVENSILEVGGDVIIEGYILNSKIYCLGS
                     IILTGATSAILGSEIIVMGGLSTYNLGSNAGVDVSVELGVHFQNERLFDEFQNKVKAA
                     DKEMANLIPQVQQANNMIKQLRGKLDEEKKKKFTELFELFKQKSANHKFVTEKFEELK
                     TSRFNQEHINIVVKGAAFPGAVIKYRRQVEKVTTMQSAFMMSFTPGQESAPMTAINVK
                     KK"
     gene            53148..54608
                     /locus_tag="PLF66_04705"
     CDS             53148..54608
                     /locus_tag="PLF66_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000810532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HMZ63372.1"
                     /translation="MKKKLSVLIGLLLVISLNTSYIFSQDTNGPTTTPTTEQHSNEHN
                     SGGHDEHKGADLALWSVIPFVVILLCIAILPISPHHIAHWWEQNNNKLLISGILGGIA
                     FGILTANGWIGKIYHTLVFEYVPFIILLGSLFYISGGIVLKGDIKATPVNNLLFLLVG
                     AFLASLIGTTGASMLLIRPLLKTNSERKHVVHTVVFFIFMVSNIGGSLTPLGDPPLFL
                     GYLQGVPFTWTFSLFPEMILSIVVLSIVYIIWDNIQYKKETKVDIKKDITKAEPISIE
                     GQVNFLWLLGVVLSVAFLNENYVPAIKHNPYIAFIREGVMILLILASYYTSNPKLREH
                     NKFTLGPIQEVAYLFIGIFVTMIPALILLESHGKELGVTEPWQFFWAAGGFSSVLDNA
                     PTYLTFLSLAKGLTGAMDVHQILANPEWEKLLKAISVGAVFMGANTYIGNAPNFMVKS
                     VAEENKVNMPSFGGYLLYSFGVLIPLFILITFIFFK"
     gene            complement(54664..55575)
                     /locus_tag="PLF66_04710"
     CDS             complement(54664..55575)
                     /locus_tag="PLF66_04710"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HMZ63373.1"
                     /translation="MHDHHHHHQPEELSDLNTRFMFGIILNILFVIFEAILGFYFDSL
                     ALLSDSAHNLSDVASLLLALFAFRLAKIKPDVKYTYGYRKTTILVSLVNAIILVFAMG
                     AIIYESIHRIKVMNSVDGNPIAIVAAIGIIINSLTAWLFLRDKEKDLNVKGAYLHMIA
                     DALVSFGVLIAGILISVTSWYWLDGAISIGIACFVLYSSIDLLRKSFHLSIDAVPEGI
                     DMVILKGEIEKVDGVLEVHHIHVWAISTTLTAFTGHVRVKKNTDIEDLLRIKQSIRHI
                     LAHANIHHSTIEFETFDEYCEEGETNV"
     gene            55798..56355
                     /gene="lepB"
                     /locus_tag="PLF66_04715"
     CDS             55798..56355
                     /gene="lepB"
                     /locus_tag="PLF66_04715"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HMZ63374.1"
                     /translation="MKSFLKKFIFFLFVVITITLIRTFVFQIYIVNGNSMLPTLKTGA
                     VLITNKYLFAQRIPITNQEFNYGSPIINRLDMVLFEGEAGDILVKRAIGLPGDFYNFA
                     DGRILIDSVPLDEKYKVPDSKTLLLPQPIVPDSVFFPMQKEGRIPPDYYLLLGDNREH
                     SFDSRNIGLIPLSRIRGKVFYILKN"
     gene            56371..58185
                     /locus_tag="PLF66_04720"
     CDS             56371..58185
                     /locus_tag="PLF66_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016760925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HMZ63375.1"
                     /translation="MKEFKDRLLFLLSCILFFFGILIIRVIYLTFLNGNMVEVKTDQR
                     VQRGIIYDRRGMELALSQDSSTIGINPAKIYSAEITANKLSKFLNMPAKKIESMILEK
                     SNYFLLKREIENSTASKILEMALPGVRVEKEFKRVYPNGTLASNLIGFTGLDDNQSLS
                     GIELLYHKELQSYVDGESLRGNDIYLTIDSLIQYRLESALGKAFAQTHSKKGIGVFMD
                     VNTGKVLAMASFPNFDPNHYSDFPVENTTNWAIRYEYEPGSTMKIFMATIMLNENLID
                     LNEKFFCPGYVEFGSRRVNCGDRHEMVDLDEILQYSCNVGIIKAIKKVPDEKIYKYLK
                     KLKFGMKTGIASDELKGRLPALKDWTPSTSYYMAIGQGFAVTPIQLVSAAAAIVNGGK
                     VLTPTVVSHIANSYGEVIKQFQYEPEYIGISQSTTQHLMRAMTKVVKQGTGVKAYTEA
                     GGVIGKTGTSQVYIKGKGYEEGMVAASFLGFFPANNPKIVGLIILSEPEGVVHSGGGI
                     AAPVFKEVVENIIPIIDLNETAVSYKLEEANTPKTKIDITRVPDFKGKTLKESLTILK
                     NYDIKYKTYGSGFNKKQSPASGEPVRNGDVWHLYFEGD"
     gene            58194..59492
                     /locus_tag="PLF66_04725"
     CDS             58194..59492
                     /locus_tag="PLF66_04725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63376.1"
                     /translation="MRYLLCLLVCFIFSQCSTTLLQRENYIQTRNALEDKDLEKAIKE
                     FPNGESNSFITSMEKAYLNLIQGKPEINDLIRYAEKIEKRVRYSASREIKTFFYVETP
                     EGYYASEHEIIWLHMLLSWGYSLRGDYEKAYVEAKVSSDLLSNAWSPEGRFDDPLMRI
                     ILASLWTLCGHWEEAQVDFRVAATLDPNLQWAQQLADLPEAPKDFILVLGGTGPEPVW
                     NPQLELNPLRGFRSLDFKSNSIKSKLTLRDSNGKTIDTHITPDSTNWYTRHEIRDNEI
                     QDLIKDSIYGQNIAITAVKEGGRSVLGVTAGVLIATGGIVLGGGIIYASVKYGSGDST
                     VDGVALGLIIMGAGISKGYSIAEEAVSESITNAKKDLDISSKYRFVRFLPDYAWVGYS
                     NQKLKTPIRILNQNQVVSEIKDARNATIVSIDYYPDVIKK"
     gene            complement(59499..59690)
                     /locus_tag="PLF66_04730"
     CDS             complement(59499..59690)
                     /locus_tag="PLF66_04730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63377.1"
                     /translation="MRTFGILKQIQSSVDGGEVKKEGNITFSKIDQYYTVKKGKELIH
                     EGVNFRIAYDYFLKLVISR"
     gene            complement(59991..60818)
                     /locus_tag="PLF66_04735"
     CDS             complement(59991..60818)
                     /locus_tag="PLF66_04735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HMZ63378.1"
                     /translation="MKNLFMKWIYLFSICVFVFSCISFERGLKSKLKYSGSNLAIYEL
                     NQSIFEKYNKEKKNIDLSDLQLKRIFLTLRKERSFIRFLDRDSLWSVETIDEMVPPLL
                     EILSLKNNKTYFVIVKEEDKTSPYARIFRTSFYLNRDDNHIQIVFGEVNDNINFGTQF
                     SFTDWANPTFFKITCRRDRLLSFYGKYEIAFKFKQDPTCSSENKPSYGNDITSLENYS
                     WILIDLNKVLEANTDKPIPVPVGNREQRLRELLKLYKKGLISKEDYELKKAEIIGEI"
     gene            60903..62591
                     /locus_tag="PLF66_04740"
     CDS             60903..62591
                     /locus_tag="PLF66_04740"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HMZ63379.1"
                     /translation="MPTFLRIFLTFTILFQIPLLAGMKEAKKAYAQKQFQKAIKLFTE
                     YTKENPSDGEPYMYMGYIYESQKDFPRSMMMFRKAVELNLNPKHRKTSYLKIILFYHY
                     HQGWDIVAHYANKFLRLDPENKEVARMRDRAYANKGHDPGVISFARPEDIAKAKQKKN
                     SEETEKLAANKEKPQEKEEPSQPKSSETPSRKTSEEKKWELSLKYFQQEDYQKADKIM
                     QELVLQNPNNKNYLYKAGIAKLRLGEYEKALKYFESSKKLTDEDDKMLRYYITLNEGQ
                     ANHKLGNTNTAIALYKKAYSINPSNLILPVLTRLYYENSYFEEVLKTSDQIPETDQNK
                     LEANMYKSLALIQTGKKRSGMKSLLDFAKKIKRIYPDINTIPEKFHEGLLQIGFFYSN
                     RIKYKLSLKYLTLVSSSKSKSSKFNFALGKTYFYTKKFDLALVFLEKVPEISAANYLL
                     AKYYAKENNLIKTKEYLSKATAKKEIYWIKVKIDPYFKDLNSNPEFMTFVETKGGKIS
                     PAKQDLPITNEKKIETNIPKIEPPATKPETVPTKDMNPSTETAPTPNLPQNTEN"
     gene            complement(62588..>62804)
                     /locus_tag="PLF66_04745"
     CDS             complement(62588..>62804)
                     /locus_tag="PLF66_04745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="flavin-nucleotide-binding protein"
                     /protein_id="HMZ63380.1"
                     /translation="YSPFNLEDLEYKKQIKGVNVLRLTPSSISAKFKFGQNRKEEEWN
                     KTVDGLIKRNNPRDSEIVDAMKQYCPF"
BASE COUNT        21587 a        11185 c        10112 g        19920 t
ORIGIN      
        1 attttttatt aaaatctatt ctgtaaaccc tttttcttat ttcgcaacag actgtttatt
       61 caaattttca cctatctgta aaatacacat taataaagca ttttgacctt ataaatcttc
      121 taatgcttga tcgattccac catgattatt tacaataata tcaattgtat ttaccgtttt
      181 taaaataaat tctaacgtgg ctttatcaga ttcacttaac atagaccaca tcctttaaaa
      241 tatacttttt ttccaatttc tccgagggta tccctattta caatgtctac ttcctttccc
      301 agaaactctc ttaattcatt cttaattaat tccaattgat acactttttt ttaatccctt
      361 ataaggaaat gtagcgtcta tcgtatataa aatatcaata tcactttttt ctgtttcttc
      421 cccgcgtgca acggatccaa aaatacctaa aagaataaag ccctgttctt ggtatttgtt
      481 ttgtattttt tttattttat ctaaaaactc ttgtttcatt acgtccattg aaccttttat
      541 cgggtttaca acatttaatg ttattatccc attgcttccg gttttgatat attggaaacg
      601 aaattctttt ccaatatata atttcaacaa taccagactt cttctcagga agaataaatc
      661 ctgttcattt tatatggaaa gggtgacttg cataaaatat acactcatta taaatttgag
      721 catagcaagc cttttttaat atgagacttt atccgaccta cacttgacag cattcttact
      781 caactgcttt caaattccct tctaaagaga caataatttt ttgttatgtc ttgtaaaaat
      841 acttacgaat ttttttaaaa ttcccgataa atcaaaatta aaaaaagtac tctttttttt
      901 caaatatatt gacgtattta tttgtactag attagataaa tcatatgttt tcttttgata
      961 attcccaaga agagttcaat tttaatggtg cacacataga gagaaatttc tatgaattag
     1021 aattcgcacg cccagaaaac aaaatagaaa tcatatccga gatcgcaaaa aaaatccctt
     1081 ggcaaacacc gattgtagat gtaatcgaaa aattaaacga ccctactcta cgtctctcta
     1141 gccgcaactt tattgatgaa attcatgtag aaagagttaa cttttgcctc aagcaaatag
     1201 cagacaaagg gcatgcaaat cattatttag atttagaaaa aggaatcttt cttttatcta
     1261 ctataggaga cagaaccgca tcctatcaaa agttcaaaga aacgttagat aaattagcct
     1321 atcgattgaa cgaactcttc cgtttaaatg atattatcct cacagatgat gtaaaagttc
     1381 atcttctctc tcgagttctc taccaagaag agggtttcaa cggaaaccaa gtctactacc
     1441 acgaaccaga caattccttt gttacaaaag tcttagaaaa taaattggga attcctattt
     1501 ctctttctgt tgtttatatc ttagttggaa tgcgtcttgg tcttcctctt tatggaatca
     1561 atcttccttt acactttatg ctttactatg aatccaatca ttacagcaca tttgtagatc
     1621 cattcaatgg aggagtgctc attgataaag acacttgtgt tcgcttttta gaggcaaatg
     1681 gctacaaaga tattccagaa tatttttcaa aggcaagcac actaaccatt ctaaaaagaa
     1741 tgtataacaa cctgatcaat atttataaaa aaacaggtcc tgaggaaatg gaacttctcc
     1801 tccaaaaaca acttggaatt ttagaaaata agacacgttt tacttaacgt tcgttttata
     1861 aagaacatgc aaatgaaggg gatgatcttt ctctatcttt ggatgaccaa attcttttac
     1921 tttctttaga ccaatttttt tcattacatt ttcagatctt tgattgattt tagcggtaaa
     1981 tgagtatatg tcgcttattg acaattgatt caaagcaaaa gagatacaag ctttccctcc
     2041 ctctgttgca tatccctttc cccaaaattc ttttttgagt ctccatccca cttccataca
     2101 aggagtgaag tcactgttga aggttacctc attgatcccg ataaaaccaa tccaggagga
     2161 tgaatctttc tcttcaacag aataaaaccc atatccattg tttttaatat tcaattgcat
     2221 tcggctcatc cattcacttg attcttcact tgtaaatgtt ctggggaaaa actccattac
     2281 ccgcaaatca gaattcattt gaattagtaa ttcacgatca ctttctatcc aattcctaaa
     2341 caataatctt tgtgtttcaa aaaatacttc tttcatttta ctattgactc actgtataaa
     2401 gaatcttctc atggaacaat gaatagacta actaaaatcc tttttagcaa aacacttttt
     2461 ttatttgtat ttttttttct ttcccttact ctctttagtg aggatagtaa aaacatgaaa
     2521 tattctacag acctatataa atcagactgg gataaagttg ccgaggacga atctaaagga
     2581 ttacccaaat cggctcttga aaaggtaaat acgatctaca agaaggcaaa agacaataat
     2641 aatccttctc aattcataaa atctgtttta tataaagtaa aatacatcgg tcaagtagaa
     2701 gaggatgcat ttgcaaaagc gatagaggaa ttacaaaaag agacaaattc ttccacatct
     2761 tatgcaaaac cattgttaca ttctatatta ggtgaagtct attggcaata ctacaataac
     2821 aatcgttatt atatcctaca acgcaccaaa acaaatcaat ttaagcaaac agatcttcgc
     2881 acttgggatg ctacaaaact cattgaagca actattgctg agtatacaga atcaataaaa
     2941 gatatcgaaa caaccaagaa aatcaaaatc gaagactttc gttcgatcct aaaccatgat
     3001 cctgaatatg ttcctacagg aagaaatcta agaccaactc tttatgactt tctagctcat
     3061 cgtgcagttg atttcttttc tggacaagaa catggaatcg caaagccagc agaaactttt
     3121 atactagata atcctattta tctttctgat ataaaagaat ttgttaaaat aaaaattgaa
     3181 acaaaagatc aaatggcata taagtattat gctctccaaa ttttgcaaga cttagcaaag
     3241 tatcatatgg aagataacga tcctgcggca ctcgctgaaa tagaattaaa gcgacttagt
     3301 tttgtttata atcatgcagt cttcgaaaat aaaggctctc tctatttaaa gtcattggaa
     3361 acaatggcgg ctcgtttctc taactctcct atctctactg acgtttacca tgctatcgca
     3421 acatattggc attctcttgc acataaatac tattcacaac caaccgacga aaataaatgg
     3481 gtctccaaaa aagcctacga tatttgtgaa tcagctatta agaaatttcc aaattcagat
     3541 ggagcgcaaa actgtaaggc attacaatct cagattaaat ctaaatcggt gactcttaca
     3601 atggagaaaa caaacatagc ttcaaaacct tttttaagtt taatcaatta taaaaatctt
     3661 gatacccttt attttagaat ttataaaatc aatcgatcta aaataaatga aatcaaaaaa
     3721 gaattttctg aatactataa taagcataaa gcctacaaat actacgatga aattttaata
     3781 tcccgttttg caaaagaaga tcctgataaa attttctcta ttaaactccc aaatgacaaa
     3841 gattatatgg agcatagagt tgaagtaaaa attccagaat tggattttgg acaatatgtt
     3901 ttatttgcct caaccgataa agctatgacg ttaaatggtc agtctatttc ttatgctttt
     3961 acaaatattt cgaatctaac ctatatcgaa aatatccgac ccaatcaaga tttagaatat
     4021 tatgttttga acagagcaac aggcgaagta gtctctgagg cagaagtgaa tacatactac
     4081 cagatttata ataataaaaa aagtagatat gaaatcaatg cagatcgttc catcacaaca
     4141 gacaaatctg gatttttcac actcaaggca aaaaaagaat ataaatactt ttatatgact
     4201 cttcgcaaag gaaatgattt tctagagcct atcgatgatt attataatgg tgatttctac
     4261 agacaaatgc ccctttatca aaatccaaat tataaagagg gcaactacaa aacagtcaca
     4321 gccaaactat ttctagatcg tgccatctat cgtcctggtc aaatcatcta ctataaaggg
     4381 attttaatag agtccaatga caaaaaaaga tcgattcaaa ccaatgttcc ggttactgta
     4441 actttttaca atgtcaacta ccaacaagta gcacaacaaa cagtaacaac gaatgaatat
     4501 ggaactttca gtggatcatt tactgctcct actaacgttt taaatgggca aatgagtata
     4561 aatacaagtt acaatgggaa tgtatatttt tctgtagaag attataaaag acctaaattt
     4621 gcagtcgaat ttgaaaaagt aaaaggtgta tatcgccttg gagataaagt taaaatcaaa
     4681 ggttttgcaa aagcattttc cggtgctaat attgatgggg cagaagtaaa atatagagtt
     4741 gtgcgtaatg ctcgttatcc gtattggtgg ggatattcaa gatggtttaa tccttctcca
     4801 caaatggaaa tcaaacaagg cgttctcaaa acagatgaaa aaggatattt tgaagtggaa
     4861 tttgaagcta ttccagatcc atccgttccg aaagaacctg gaatttcttt ttcctatcaa
     4921 gtcaatgcag atataacaga tttaaatgga gaaactagat ccgcctcagg atttgcctat
     4981 gtaggttata cttctttaat cgtaaatgta aatgttcccg aaatggtaaa cctaacgcac
     5041 gaatctcctt ttgaaatctc tacaatgaat ttgaatggag aatttgaacc ttcacaaggg
     5101 tatattacaa ttcataaact caaatctcca gctcatgcct atcgtagaaa aatgtggcag
     5161 aagccagaca agtttatcat atctaaaaat gattatgtga aagacttccc aaatgaagta
     5221 tatgataaag aagacgatgt ctctacatgg gagaaggaaa aagaagttct aaacactcca
     5281 tttaacacag aaaaagaaaa aaatctcact ctaaaaaatc tatctaagtg gctaacagga
     5341 cgttatgttc tagagatcaa atcattggat aaattcgggt ctgaagctaa ggaaattaaa
     5401 tactttacag ttttctcaga aaataccaaa gatccaatcc caacaccaac ttacaattgg
     5461 aaattatcca ttaaaacagt tggcgaacct ggcgaaactg ctcgtttcat tttaggttcc
     5521 actgaatcgg taaaaatttt atatgaactt gaactagata aaaaaatcat tacaagagaa
     5581 tggatgtctt ttgaaaattc acaaaaacaa attgaaatcc caataaaaga agaatacaga
     5641 ggcaatattt atgctcattt tgttcttgtg aaagacaatc aaatttttac ttctagcgag
     5701 caaattactg ttccctacac caataaagaa ttaaaactga gtttcgaaac cttccgaaat
     5761 aaactacagc ccggcgaaaa agaagagtgg agaattaaaa ttcaaggtgc aaagaaagaa
     5821 aaagtaatct cagaaatgct ggctacgtta tacgatgcat cccttgatat gtttcgaaaa
     5881 aactattggg atttttccat ttatccttat aattattcct cttctaactg gcaaaatcga
     5941 aatgatttta atactgaaaa ttttagacaa tttcaaagag attggaatgt tgcgtcctat
     6001 ggaattagca agagctatga ttatttaaac tggtttggac tctatctcta cggctttcgt
     6061 ggttactatc cataccaaac tcgatcagtt agaacatacc gaaatggaag aagagataaa
     6121 gaagcagaag tagagtatga tttagatgat gcaccaatgg ctgaagaaag agcagctcct
     6181 gcgcccgcaa tggcacaatc agctccagta gcaacgggtg gagtaaataa aaaatcagct
     6241 atgagaaagg aatctttcgc ggctgacgct ccaggtgaag caggtccgga actaaaagaa
     6301 aaagcagaat caacctcatc taacacaaat aatcaaagca attctggagg gaatggggaa
     6361 atcagtggag taaaagcaag aaccaacttc aatgaaacag cattctttct gccacatctt
     6421 atctctgata aaaatggaga ggttactgtc tcttttacaa ttccagaagc tttaacgaaa
     6481 tggagaatga tgggcttggc tcatacaaaa gacttaaagt atggttttat ttacaatgaa
     6541 cttgtcacgc agaaggaatt aatggtagtt cctaacgctc ctagattctt cagagaggga
     6601 gacgaaatgg aatttacagc caagatcacg aatctttctg ataaaaaaat gtcaggaaaa
     6661 gcgcagattg aattcttcga tgcaataact ttaaaaccaa ttcttcacgg aaatatttta
     6721 aagacagaga agttacaaag ctttgaatct gaaaaaggac aaagcacagt tgtaaaatgg
     6781 aatctaaaag tcccagaagg gttacaggca attagctaca gagtattggc taaatcagat
     6841 aatttttcgg atggagaaga gatgcctctt ccagtcctta ccaatcgaat gctagtaaca
     6901 gaaactcttc cacttccaat gagaggaaaa gatccaaaga attttaaatt agaaaaaatg
     6961 atttctaata aatcagatac gttagttcat cataagtata ctttagaatt tacctctaac
     7021 ccagcttggt atgctgtgca agctcttcct tatattatgg aatatccgta tgaatgtgca
     7081 gaacaactct tcagtagatt ctatgccaat agtcttgctt ctcatattgc aaatgcaaat
     7141 cctaagatta aaaaaatatt tgaatcttgg aaacttgcag ctgttagcaa taaagaagat
     7201 aattcaaagg cacttctttc taatcttgaa aagaatcagg aactaaaaag tctcatgtta
     7261 gaggaaactc cttgggtctt ggatgcacaa gatgagacag aaagaaagaa acgagtagga
     7321 cttctttttg atttagcaaa aatgtcagat gaattagaaa gagctcttac aaaattaatg
     7381 aaagtccaaa gtagcaacgg aggctttgct tggtttaacg gaatgccaga gaaccgttat
     7441 atcacacagc atatcgtaac aggccttggt cacctagacc atttaggagt aaagagagtc
     7501 agagagaatg caaacgcatg gaatatgaca acaaaagcag tgcagtatct agattggcag
     7561 tttaaggaag actatgatcg actcatcatg ctagaaaaaa gagacaatct aaatatgtcc
     7621 tcacaacaaa tctggtatct acagattcaa tatctctatg ctagaagttt cttcaaagat
     7681 gttccaatgc caaatgaaac aaaagaggca tttgaatatt acaaatggca agccaagacc
     7741 tattggacaa gctttctcgg gaataaaatg tctacaggaa tgattgcatt agcactgaat
     7801 cgactaggag aggaaacgcc tgacgcattt acaaaggcac tctcttctca taaactaaaa
     7861 gattttaaga gcaataagac tcctgaacaa attgttaaat ctctcagaga acattcactc
     7921 aattcagatg aaatgggaat gtattggaaa caaggatggg gttatttttg gtatgaactc
     7981 ccaattgaaa cacaaagtct catgattgaa gtctttgatg aagtagcaaa agatgaagat
     8041 gcggtcaatg acctcaaaac ttggctactc aaacaaaaac aaacccaaga ctggaaaaca
     8101 acaaaagcaa caagtgaagc gatatacgct ctactcttaa aaggagagga ttggttacaa
     8161 acagatagaa gcgttgaaat tcaattgggc tctaaaaaaa tcaatccttc cgaggataaa
     8221 gatattaatc ttgaagcagg aacaggctat ttcaaaaaag cttggaatgg ttcagaaatt
     8281 tcaaacgata tgggaaatat cacagtcacc ccaaaaaaca atactctcaa aactaaaaat
     8341 gctggagtta gttggggagc agtttactgg caatattttg aacaactaga caaaatcact
     8401 cctgcggata ctcctctcaa gttagaaaag aaattgttct tgcaagaaaa tacaaaaaca
     8461 ggtccggtta taacgcctat cgacaacaag accaagctaa agccaggaga tttgataaag
     8521 gtaaggattg aacttagagt agatagagac atggaatttg tgcatatgaa agatatgaga
     8581 gccgcaagct ttgagcctac gaatgtgatt tctcgttata aatggcaaga tggattgggt
     8641 tattatgaat cgactagaga tgctgcaact aatttctttt ttgattcgtt accaaaagga
     8701 acttatgttt ttgaatatcc acttcgtgtt acgcataaag gaaattattc gaatggtatc
     8761 actacaattc aatgtatgta tgcacctgag ttctcaagtc actcagaagg tgtaagagtc
     8821 aaagtggggg agtaataaat gtctacagaa gaaaaaagtt cggaaagaaa atcacgcaga
     8881 ctcaagatta aatgtcctat ttgtaaatca gaggatgtag agagaaaaca agctggctcg
     8941 ttcggtggcg aattttatgc aacctaccat tgcattagct gtgatcattc tgatacggta
     9001 aatgagcaga aattagaaga atggtttgga tattaataaa ccatttcgaa atggaggata
     9061 atcgatgtta gactttttcg caggattagt ttacgcaaac ggacttgaat ggtttgctca
     9121 taagtattta ttgcatgaag agggaaagaa aaaagatagc ttctggcgct ttcattgggc
     9181 agaccatcac aaaattgtaa gacaaaatga ttttaaagat atctcctatg aaaaaagtct
     9241 gctagaatgg aatccgcaaa gcaaagaggt tcttgctctt gcaacggcag gtattttaca
     9301 tttgccgatt gctctgattt cacccgcatt tgtagcagga gtttttgttt caggatccta
     9361 ttattactat gtgcataaga aatcacatct agatccagat tggggaaaag aaaatctacc
     9421 ttggcactat gatcatcata tgggacccaa tcaagatatg aatttttgtg tcacatttcc
     9481 actctttgat catattatgg gaacgagggt tagatacatt ggaacagaga gagaaaaaga
     9541 ggatcaaatt agaagaaaac aaaagttagc cgcaatatga taatgatccg aaaaaataaa
     9601 aacaaaacaa aagagaaaat tctaaaagta gcaatggatt tattttacga gagaggtttt
     9661 accaatactt ctgtgcaaga tatacttgat agatgtaaac taacaaagcc atctttctac
     9721 tactatttcc catctaaaga tcaattagcc attgaatatc taactaaaca ggaagaggtg
     9781 aatagagatt ttctaataac tctaatggat tccagtaaaa caccagaaga ctttgcagtt
     9841 aaatgggttg ctgtcattaa aaaatggagt aaggttaaaa aatacaatgg ctgtccgttt
     9901 ataaactttc taagtcaaat cgaaatttct aataataaat ttaatacaaa gctaaaagaa
     9961 attgaagtaa actggacttc tctttataaa aattatttaa tagagcaatc tgaaaaaggt
    10021 cttatagata aaaccattaa cttcgataaa gctgcacgaa aaatggcact catctatgaa
    10081 ggatcggtta atttatggaa aatgagtaga gacgttaagt atttagattt tatggaagaa
    10141 gagcttaaag aactcatgca ttcaattcgg aataaaaaat gaaatacaca attgtaggac
    10201 taatcttttt cggattactc attattagct ttaattacac aattgtagag aaaacaaata
    10261 cagatttaat ctatccttac aggatgaaat tatattatac gaagattgat ggattaagag
    10321 aaggaacaga agtatctgta aaaggtgttg attttggact cgtgcaagaa ataaagaaag
    10381 tcccgataga agaagttcct gatcaaagat ttttagaacc tgggaagtca catgcaatag
    10441 agctaacact cattatgcgc tcacctatta ccctttggga aaattatgat gtgaagttta
    10501 aaaacaaaac aagcttttca ggtagaagca ttgatataga tcctggctac catcaaacag
    10561 aagagagtaa attcttccat ccaacatata caaaagagga aaatcctcca gcgcactccc
    10621 cttctgccaa atactatgat gatttttttg tagcttcaca caatacacta gaagaaaata
    10681 gatccgatat tcgtagaaca atgattaacc tcagagagct ttctgttaaa ctcaaagggg
    10741 aaaaaggcac tttaccacaa cttacaaata cagacctcgc ttataatgga ctattagata
    10801 caactgcaga tgcacgaatt gtcttaaaag agtttcgtag ataccaagag ggaatcagag
    10861 aatcagatac gatttttatt ccattctcac ttgtgcttta tagacaactc gtaggctcca
    10921 tttatggaaa ccaatactaa ataacatcag tttgatgtaa caaagaaaaa tgaataaatt
    10981 taaaaccatc tgtgttcatc cgtgtttatc tgtggttaaa aggatcccta aatcggactc
    11041 gcgttagact cgacgcatcg tttctctaag caaatcaaaa aggaattcgc ctgttatttc
    11101 tccaggaaca accacacgat aggctccccg tttcatcagt ttcttttttt gttccatttc
    11161 gtctaccgtc atgatatact ttgcttcagg agcaatcttc tttagttgtt caagtaatct
    11221 ttgattcgta atacctttta agaacacatc tgaaatggag caaataataa tcgaagcatg
    11281 gtcaatccct atatgcgtaa gcgtatccgg atgcgctaaa tcagcatact cccatttaaa
    11341 acccttatcg tgtaaaatat tcttattcgc tggattataa tctacaacta aaattcgttt
    11401 cgctaaatcg ggagatataa catgaatata atcaaccagt gactgaccaa ttctaaaata
    11461 tccaagtaaa ataatatctc ttttcgattc atcgtgcata ttctctatat taatatgatg
    11521 ctcttttttt ctatcggata aaccaaatat ataaaaagct ttcattaata aagcagcaat
    11581 ctcgtgatta aataagatta aatacgtaga taaaacagag gagagaatca ttgagtttag
    11641 aattagagat ctaacgtcag gcgttacatg accataacct tgccctagag ctatgatcac
    11701 taaggaaaac tcactgattt gagaaagatt taaagaggtt acaaaaccat ttctaagccc
    11761 tcgttttgca gtgtaaacaa tcggagcaat cgaaataaat cgaatcaata caacaaagag
    11821 agatacaacc actgccatcc caatcataga tatagttggc attggcactt ttagtccaag
    11881 agaaacaaag aataatgtta caaaaaaatc tctgattcca atgagtttag aaataacgtc
    11941 agttccatag ggataagcag ctatactcat cccagcaatg agcgcaccca tttctttgga
    12001 caatccagcc atatcagcga caccacacaa tccaaaacac caagaaatgg aagttagaac
    12061 aacaagctca ggtcgattag ctacagattc atagattggt tttaaaataa atcgacttac
    12121 taaaaatgca aatacgagaa taacattgat tatccctatt gagagaagga ttttatcaaa
    12181 ctctggagaa agtaaattcg gttgggcacc cataaataaa atagcccaaa tatcttgtaa
    12241 aacaagaact ccaatcgtta atttaccaga tacggttgaa atttctactt tatcctgtaa
    12301 aagttttact acaattaaag ttgaacttaa tgataaagcg acacctatat acagtaaatt
    12361 aaaattacca cttacgaatc tataaccaag caagaaaaag aaggcaatac ttaaaaaaac
    12421 acaaataaga aattggaata ctccaagggc aaacattgat ttacccatct ttgcaagttg
    12481 gtttaagttg atctctaatc caataataaa gagaagcaaa atcaatccga tctcagaaat
    12541 aagctcaata cttgcttcac tagttacaag tccaaaaccc atttccttcc caagaattac
    12601 ccctcctaga atataaccta atataagagg ctgttttaga agacgagcga tatgcgataa
    12661 gattgtcgca aatataattc caagagcaat gtcgcgtagt aaactaattt cggatccatg
    12721 cattaatttg tctcttcccc atcaaagtta taaatttttt gtcttccgct tgctttttga
    12781 aaccgcgttc caattttctt tccagaatag gcgctgtagt aggttataga atctacaaaa
    12841 tcatataaaa ttacattatc cccggataag tgaacatttg cctttgcgag tttaggaatt
    12901 cccactctcc aattttgctt accagaacga agatcaattg cgattaaatc taaaggtgtt
    12961 gcaatataaa gagttgtctt atctaccgtt ggatgtggta aaattggtcc attcaaatga
    13021 actttccata aaagagaacc gttatccgca tttaagcaat aaaacattcc atccgcatct
    13081 ccaaaaaata ccttatcatg aaatacaacg ggatttgtat tcatcttatg attggctaaa
    13141 tgaagccatt ttttctttcc atatttttga gaaatagaat aaatatttcc gtctgtgcta
    13201 ctaaatacaa tctgttcttt agttcccgcc gcagaagtca atatctcacc agccgtattg
    13261 tatttccaaa cagttgcacc agttgtttta tttaaagcgt ataacgctcc atcctttgaa
    13321 ccaaatacta ctttatcgtc taagataaga ggctttgcat aaatagccgc tccaagtgct
    13381 cttctccatt taattttttt tgagggataa atttcttcgc aaaaaaaatc gccctgttcg
    13441 tttccataat atttatattc tccatccaca tgcgacaaat cttcttgggt atgatttaaa
    13501 agcagattgc aggactttga ctcagcacaa ttaaaattat aggcaaactc agatggtaaa
    13561 ctccctgact gagaaaaaag ttttgatgta tttaaaatga ttagtaaaaa aataatctgt
    13621 ttcaattata agcctcctat tgggtctatc tatcctaaat aaatttaccc ttatctaaaa
    13681 atcaggtaac accgatttac cagatgaatg gcttctaaat ataaatttag aaaatctatg
    13741 aaagactctg tatctagttg gtaaaaggtg atatacaaat taaatctacc aatattatcg
    13801 tccaaatcat aaaaaccctc gatttcaaaa tgactctgta ttttgagatt cagcaaaaat
    13861 actaaagcag taagcaaata aggattattt gaatattata gaataccaat aaaatcaata
    13921 gtctcaaacc aatcggttgt tctaaaaaat atgacaaatc tatctatttg ctctctgcca
    13981 atttactctt tctaattctt tctttaccac tcaatacgac caacaaactg aaactgaaat
    14041 tgattaatct tttaaaaacc atgatattac aaaatcaaag ttaaatttga aactgtctaa
    14101 aaaataaatc ttataattta atttattctg attgagttat tttatttaaa tttcaaatta
    14161 agcattgaga aataatgaaa aaatttttat ctaccatctg tttaattcta ttagtatcac
    14221 ttcatcaatg taatctcaga tttaaaagaa tatatgaccc caagcctatt ggatctatag
    14281 gttctcaaat tgaaaataaa aatataacaa gaacaaaact tcaatttatt gtaattggcg
    14341 attggggaga aaaaggcaat ttctatcaaa aagatgtagc atctgcgata gaaaaaatca
    14401 tgagggagcg caaaatcgat tttatcctca ctaccggtga taatttctat cctaacggtg
    14461 ttgaaaacat atctgattct cactttaaga aatcatttga ggagatttat tcatttgctc
    14521 aggagacacc atggtatata tcgcttggaa atcatgacta ttatggaaat atccaagcac
    14581 tgattgatta tcaaaaatca aataaaaatt ggattttacc ttcaacatat tattcattga
    14641 ttgctaatgt tgcagataca gatgctttat ttctaataac agacacaaat gaatttataa
    14701 aaattgctgg cttcccctat agaacttata tagactctaa tttgaatcaa atacaaatca
    14761 agtggatgaa tgatacatta tcacaaagtc tatatggttg gaaaattgta gcaggacatc
    14821 atccaatttt ttctggtgga gatcatggtg atacagaagt attaaaaaag gactttctag
    14881 atataatgga agcaaatcaa gtagatatct acttttctgg acatgatcat gatctacaat
    14941 atcttagaca tcccgactat cgcactcatt tttttatttc aggagcaggt tccaaagttc
    15001 gcaaattaaa aaataatcca aataccgtgt ttgcaaaaag tgaagcaggc tttattctaa
    15061 ctacccttga aaataaaact gctaccatag agtttgttaa ttcatctgga atagagattt
    15121 ataaaactaa aatagaaaag taatctactg aaaagaattc gccaagagtt cacaaactct
    15181 atctatttct tttccattca caatggtttg aatccaagtc ttaggaatag agtcataacc
    15241 ataatgtaga gcagcaatac cacatgcaat agccgctata gtatcagtat catctcctaa
    15301 atttactgcc ttaattgctg tatctttata attatttgaa ttgagtaaac accaaagaga
    15361 agcttctaat gaatcaatga caaatccact cgacttaatt tgagaaattg aaaaagtttt
    15421 gagatcattt tctaagatat ttttaaaata agactgctct gattgattta agtaattgta
    15481 gtatttattt ttattctgct ttatttgatt aagagattct tcaatggaga atcccttcaa
    15541 taccaataaa gctaggtcta tgtaaataag acaggatata acagatagag aatgtccatg
    15601 tgtaattgca gaaacttcct taattatttt atactgatct ttatcaggag tgttcttaaa
    15661 gtaaaaatac aaagcaagtg gaagtgttcg cataagagaa ccgtttccat tcgtttctat
    15721 tttggaatct ccagattgat aggcagagat accatttatc agcctattta ctgaattcgt
    15781 tgtcgaacct ccaacgtcat acgcactacc aaatggagta aacttcccat ttttatacca
    15841 ctctataaag ttatgcgcta tattatctag attatagcca cgacttaagc tttccgcaat
    15901 acataaagta agagatccat catccgacca acttcctggc ggcatattaa aagtcccata
    15961 tccaatcata tcgttaaccg gatttttttc taggtaagtc cttgatttaa attgaacggg
    16021 aagacctaaa gcatctgcta cacaaactcc ccatatccct gctttaacaa tttcgagtgt
    16081 attcatatat atcctagtaa gttgtaaatt tatattctgc aagtaaacat tctttataat
    16141 aaagttgaaa actccaattc ccaggtaatt tttcaaatgc aaatttatct tgaaacacaa
    16201 agctatcttc taaatatgcg atagtcgctc cagattcctt ggtaatagta gtgtctgcgt
    16261 tgtgagaatt aaaaactccc tttttagatc gcatagtcgg gtgaactgtc ttgattataa
    16321 atccatctat ctgagtgaga tgaattggca aattactaac ttcgaaaacc acatagggca
    16381 tatatccatc tttcatctca actctattac ttttcgagag aatttttatc tcttgtatat
    16441 ttacaaggac tcctgatgca gtatacttag aactttcttc actcattact tctacaagtt
    16501 taactccaga ctctacaatt tttgctttca gattccaagg attcacacaa gattttttct
    16561 tttggtgaat tggattctga ataatagcaa gacttaaaaa gaaaagaaat atctgagata
    16621 gcatagggtt actcttattt taccgctaat tcctttttcc aataattgac tgttgcttcc
    16681 gtgctagcca tcgatagaaa ggaaacccag tctttatact tttcctctac ctgttttaaa
    16741 tctgatttcc cttctgctgc tgtttttgta tcaaagaaat aagtttgaaa cgtctgaatt
    16801 aattctttcc caccaattgg tccatgccca ggaattactt tttcgatctc atattcttgt
    16861 aatattttat tctgcttctc taaataattt gcaatcctag atccatcttt ttcctttaag
    16921 aatggatgaa gacctatcat caaaagatcg cccataaaga tcatttttcg attcttaaaa
    16981 tatacaatca tatcatttct tgtatgcgct tgaccaatgt tctggagaat aacggtctcg
    17041 tttcctatct gaatttcttt tttttcttta atccattcag taggaattcc aagatctcca
    17101 ttttctgcaa tccagtcttc tcttgtatag gctcctgcaa tgatagtatt acctttgtat
    17161 aattcattcc cgcctgcatg atcagaatga aaatgtgtat tcactaatag aattgatttt
    17221 gagcctgctt tttcttttac cagagcgtat aatttatctt tgccgctaga aatttttgat
    17281 tcaacaacta gaacttcttt ttctccaatt aaaattgcag aatttccccc tcctcccata
    17341 actaaggtta gattcttgtc atattccgcg acttcatact gattcatgaa ttttaaaaag
    17401 ggaaatactt ttataaataa aacaattaac gcaatagtta aaaatcccaa tacgatccaa
    17461 ataacttttt tcataactct ctccaactga ctctagacta aacaataacc ctataaatct
    17521 gcaacggttt ttcttttccc tttacttcaa cgggtggaag cttttcgact tcaatttgtt
    17581 ctttcacttt cagatatact tcttctgata ttaaaaactc tgcttgaaat tttttacaaa
    17641 gactttcaat tctagatgca gtattcactg cgtcaccaat aaccgtatat tccattctac
    17701 cttcaaaacc aatgctaccg gtaaaaactt cgcctgaatg caaaccaatt ccaatttgaa
    17761 tttcttcctt gccatgattc ttacgaatta aattccactc cttcagtgag gctaacatct
    17821 gtaagccagc cttcacagca ttgagtggat catcaggtgt agccactgga gttccaaagg
    17881 ttgccatgat agcatctcca atatatttat ctaacgttcc ggaatttgca aaaacaactc
    17941 gagtcattct cgctctaaat tcatttaaaa attctactag ttctacagga tccatttttt
    18001 ctgacatctt cgtaaaattt cgaatatctt gaaataaaat ggtgacctct tgcctttttc
    18061 cggtgtgtaa tatatcagga tcttttgtaa tctcttctac aatcatggga gaaaaatatc
    18121 tactgagaga tactttttca gattcaattt tagctattct acgaaccatg tatacggtgc
    18181 gatagatgga ataggcaacg ctaattgcta tacaaaagaa aacaaggggt cgaccaatta
    18241 aaatatcact tagaaaaatt ccattgccta gtatataatc gatccaatca taagagagtt
    18301 ttactccaat atagagtcca taaccgagcg aggcaaaatg gattgtaaaa aaaagtataa
    18361 ttgaaaaaat tacaagccta attcggattt gaattaatac caagataaat aagagaaaaa
    18421 aataatcaaa cccaggattt ttcaaaaagt aattaaaatt atcaggactt tgcatctgtg
    18481 accaagcaaa catcatccct gtcaaaacag aataatcgaa gattattgta atatagccaa
    18541 agaaaatatt taaactgtta acatttttag ccaagatgat agaatgagct agagtcagca
    18601 aaaaataaat taagattcca atcccatata tcttaacacc gtagtaaaaa agttcggaat
    18661 ccgttagggt gtaaagatta atgactgtcc ctattaaaat gaaaacaaga aaaatgtatc
    18721 tgaaccgatt tgctatatag gcaccatatg cctcttcttc ttctaagatc ctgcgaacgg
    18781 caggcgccat caatcttctt tctagaacat cacgaatgtc attaaacatg gattatttct
    18841 tagctggatt tgcatccagt tctggaacta caggttgaga aacaggagaa tcctcttttg
    18901 gaaatacatt atcaggatct ttgtcgattt ttgtttttgg atccggaatg atttcttttt
    18961 gcgcatcaaa tcgagcataa ccaataaacg cactatcttt taaacattta ctttttttat
    19021 ctccttcaat ttctctcaca cagccaattt tttggtttgg atcaaaaaaa taaatatcag
    19081 gtcccccgcc ttttgtaaaa agaccatcgc cccaagtatt agatcctgcg acacgcataa
    19141 cgcccgtttc atccataccc atataaatca tgacatgact tactcctgga tctctatcag
    19201 gagggacgtt atacgaattt tgccagtaaa gtaaatctcc aaattgtaaa ttttttttca
    19261 attcttccgt attaatcttg ttatccgctg tttttggggg ttcaaaaagc cagccttttt
    19321 ctttgaaaaa attaaactgt gaataggaat ctctaggcat tgtcttactc aatgctttgc
    19381 tatataaata ttggacagtt ccggaacaat ctaacttgac aggtttatta ctatcaggtg
    19441 gagtccaagc ctttgagtat tttatatcga agagtgcaag ccattctgct gtcttcgtta
    19501 aattatctct ctctgctttc gataaatctg catataaaga gattgtgatt aagaaaaata
    19561 cgatgaatcc tttcatagca gtcatcgtat ttaatttagg aagaaagcaa gaaattttta
    19621 ttttaagcta tagatttgat caaaaagtcc cttatcacta aaatgttttt cttgtgcttt
    19681 tttccaacca cctaattcat ttatagtaaa tagttttaga tctttaaacg tagatttata
    19741 ttttttcaaa acagaatcta agctagggcg atagaaattt tttgcagcaa gctcttgggc
    19801 ttctactgaa tagagatatt ttagatactc ttctgagact tctcttgtct tatgcttatc
    19861 aacattcgca tcaacaatcg aaaccggagg ctcagctaaa atgcttacag atggagtaat
    19921 aatttctaca ggatcttttg catctgcttc cctaagagag agatatgctt cattttccca
    19981 attcaaaaga acgtcaccga tttttcgatt gataaatgtt gtagtggcac ctcttgctcc
    20041 agaatctaag accttcacat ttttatacaa agcagtcaca aattcttttg cctttgtttc
    20101 atcattttta tttgccttca atgcaaatcc ccaagcagct aaataaatcc agcgtgctcc
    20161 accgcttgtt tttggatttg gtgcaataat ctccacaccc ggcttaatta aatcactcca
    20221 atgctttatt gctttaggat ttccctttct taccaagaac acaatggttg aagtataagg
    20281 ggagctacta tttgttagcc gcttctgcca atcagcggga atttttttgg atttctctgc
    20341 aatcgaatca atatcatagg caagtgctaa ggttacaata tccgcctcca atccatcaat
    20401 cacagatcgg gcctgctttc cagaaccacc atgtgattgt tttacatcaa tttcttttcc
    20461 cgtcttagtt ttccaatatt tcgcaaagag ctcattgtat gctttgtaaa attcacgagt
    20521 cgggtcatag gatacattta agattgagtc tttagcggat aatgatatta taccgctagt
    20581 cattgcaact aaaataaaaa tcataacatt ttcaaaatta tatttagttt tttgagagtt
    20641 cattttcata ccttttaaat ttgcaaaaat tttctaatct agcataaatt caatataaaa
    20701 actttttcat tactaatgct tacatacaaa gaccaatgct ataagctctt aaagattacc
    20761 atggataaaa tattaagaat tattaaaacc atctgtgttt atccgtgttc atccgtggta
    20821 aaaaatctta catcgaattt gcatttatct atttgaacca gaaagcaaat ttctttttaa
    20881 tacacccaat attcttttat atcatactaa gtcgagtaat atatttgaca aaaagtttaa
    20941 tatattggat atggtattca tgttcggaaa gaaaaaaagc attctacctg gatttggaat
    21001 ctctctaggt tatacgattt tttatctaag tatcattgtt ttaattccaa ttgctggatt
    21061 atttattcga acttccgaac taagcttcaa tgaattttgg tcagtcatta cagaagaaag
    21121 agtattggct tcttataaaa tcagctttgg agcctccttc tttgccgcaa ttacgaatct
    21181 catttttgga cttttaatcg cttgggtttt agttcgatat gatttctttg gaaaaaagat
    21241 ttttgatgcg ttaattgact tacccttcgc ccttccgact gccgttgcgg gcatcacact
    21301 tgctacaatc tattcaaaaa atgggtggtt cggaagattt ctagaaccta tgggatttaa
    21361 tattgcatat accccgaagg gaatttatgt agcacttatt tttattggtc ttccatttat
    21421 tgttcgatct gttcaacctg ttttagaaga attggattca gaagtagaag aagcagctgc
    21481 aagtctagga gccactcgac tgcaaacatt cataaccatt attttaccag ccctattccc
    21541 ctctcttcta agtggattca ttttagcatt tgcaagaggg ttaggagaat atggatctgt
    21601 gatattcatt tcaggaaata tgccaatggt aagtgaaatc acacctttgc ttatacttac
    21661 aaagttagaa caatatgatt atgctggggc gactgctcta gcggtttcca ttttactcat
    21721 gtccttcttt ttattttttt gtatcaatgt attacagtgg tgggcggcta ataggcataa
    21781 ggcgtagtat atgaaaagag atacaagaat cacagagggc tttcttacta agtttgtttt
    21841 aatttcgatt gcacttcttt ttatctttat cgttctaatt gttcctcttt tttctgtatt
    21901 catttatgca cttgataaag gatatgaagt atacattgcg agcattcaag atgaagaagc
    21961 gttatccgct ataaaattaa cttttcttac tgtcctattc acagtcccac tcaatttagt
    22021 ttttggaatt gcagcctcct gggcaattgc acgatttgag tttacaggaa aaagtctatt
    22081 gactactctc atcgatattc ctttttctgt ttctccagtc atttcaggtt tgatctatgt
    22141 tttactcttt ggtgcgcagg gactatttgg aaattatcta gccgagcatg atataaaaat
    22201 tatttttgca gttccaggaa ttatcgtagt tacaatcttt gtaaccttcc catttatcgc
    22261 tcgtgagtta ataccgatta tgcaatcgat cggtcatgag gaagaagaag cagctctgat
    22321 ccttggagca aatggctggc agatgttttg gcatataaca attcctaata taaaatgggg
    22381 tatattatac ggtgtaattt tatgcaatgc aagagcaatg ggtgagtttg gagcagtatc
    22441 tgttgtatca ggtcatatac gaggactaac caatacaatc ccacttcacg tagaaatact
    22501 atacaatgaa tacaattatg ttggagcatt tgcggttgcc tccctactta cgttagccgc
    22561 attagcaacc ttagttataa aagaaatcat aaattatttt agtcataaag cggatagata
    22621 aataacaatg agcattgaaa ttaaaaatat acaaaaacac ttcggaaaat ttcacgcaat
    22681 caaaggattg aatttagata ttaaagctgg cgaattagtt gcacttcttg gtccttctgg
    22741 ttgtggaaaa acaactctac tccgtatcat tgcaggactt gaaactgctg attccgggtc
    22801 cattttattt tcaggagaag atacaattga taaagatgtt ggagaacgaa aagtaggatt
    22861 cgtgtttcaa cactatgctc tcttcaagca tatgactgta tttgaaaatg tagctttcgg
    22921 tttaaaagtt cgcccaagaa aacaaagacc ctccaagcaa gaaataaaag ataaagtaga
    22981 aagcctttta aaacttgtaa aactagaact tttacaagat agattccctt ctcaactctc
    23041 tggtggtcaa agacaaagag ttgcacttgc tagagcactc gctgtcgagc cacaagtatt
    23101 gttactcgat gaaccttttg gtgcgttaga cgcgcaagtt agaaaagaac ttagacgctg
    23161 gctcagaaaa ctacatgatg aaattcatat cacaagcgta tttgtaacgc atgaccaaga
    23221 agaagcatta gaagttgcag atagagtagt tgtaatgaat caaggacaaa tagaacaagt
    23281 aggaactcct gaagaagttt atacaaatcc agcaactcca tttgtttata actttctagg
    23341 aaatgtaaat ctatttcatg gtcgaattga aaatggtaaa ttatttcttg gagacaaaga
    23401 aattaaacac acccaagaga caaacgcaaa ggacaataaa gcatttatct acataagacc
    23461 atatcacttt gacatatacc taacgcatca aagtgataca atcgctgctg ctcgaatcga
    23521 gcatattagt cccgcgggtt caaatataaa agtagagtta atcgatgaat tcagtcaacc
    23581 ggtattagtt gaaattccaa tggaacgtta tagagaactt ggaatcaaga aaggggacaa
    23641 tgtttatata agcccaaaac aaacaaaagt tttctttgat ggggcttacc aggactattc
    23701 gatataaact aacgtcaact cactgttaaa atatttaaaa gattaccacc gatagcaccg
    23761 ataactgaac gagacagaaa cgattgataa ttttggatta tcgtatcatt tatcggtgtt
    23821 catcgtcttc atcggtggta atctttgcaa atttccctta acttgacagc attgactaaa
    23881 gccttaatat gcgatcgctc gtgaagaatt tttacatcga attcacgcta ttttatttat
    23941 gaatctcctg taatgagcgg attgtaaatc ctttattacc catttctaag agtccatcca
    24001 ctagagcttt tgccgcctct gctgttgtaa gacaaggaac tctatattta atagcagcct
    24061 gtctgatcgc aaacgcctca tctctagtaa ctcgactgag tggagtattg attatcaaat
    24121 taattttctt ttctttgata aaatctacaa cgtttggaaa tccttcatgt aatttattaa
    24181 tcacagaaga aatcactcca ttatcagata agagtttatg agttcccttt gtggctaata
    24241 ggttgtaacc cagcttagaa agtttaacaa tataagggag caactcaggt tttgttttat
    24301 cattgatact aacaaagatc gttccaccct taggagtttc ctctcccgcc ataatctgag
    24361 cttttaaata agcctcgcct gcggtagacg caatccccat tacctcacca gtagatctca
    24421 tctccggtcc aagaattgta tcaacgcctg ggaatttatt aaatggaagg acagcctctt
    24481 ttacattcac aagtttgaca gaatattcct cttgtgggat gagttctttt aatttcttac
    24541 caagcataat cattgtggca tacttaacta ttggaatccc aattgcttta gacacaaatg
    24601 gaactgttct cgaagctctt ggatttactt cgagtatata caaggtttca tctttaattg
    24661 caaattgaat attgataaga ccttttactt ttaattcaag tgctagagcg cgagtcgccg
    24721 cttttatttc ggaaaggatt ttatctgaaa tgttttgcgg aggaagcata caagcagaat
    24781 ccccactatg aacaccggct tcttcgatat gttccatgat tcctgcaata aatacagttt
    24841 cactatcgca caacgcatct acgtcgactt caaccgcact atccaagaaa gaatcgatta
    24901 gaattggtct atcctctgaa atctcttctg cttcatccat gtatttgtct agaccggcag
    24961 tatcattgat gatagccatc gctcttccgc ctaatacata actaggtcga acgagaaccg
    25021 gataagaaat ttcagaagca attcttcttg catcttctct tgtagaagcc attccatttg
    25081 caggttgttt taaaccaagt ttttgtaaaa cttccacaaa gcgttttcta tcctctgcac
    25141 gatcaatcga atcagggctt gttccaagaa ttggaactcc tcgtttttct aattctgttg
    25201 caagtttgag aggtgtttgt ccaccaaatt gaacaatcac accaattggc ttttcatttc
    25261 gatagatgtt cattacatct tctaaagaaa gtggctcaaa ataaagacga tcggatgtat
    25321 cataatctgt agatacagtc tctgggtttg aattgaccat aatcgattca acacccatgt
    25381 cttgtaaagc gtatgacgca tggcaacagc aataatcaaa ctcaatccct tgtccaatgc
    25441 gatttggtcc tccccctaaa atcatcacag atttagtagt ggttacattg gattcatctt
    25501 ctgtgtcata actagaatag aagtaaggtg tataggcttc aaattctcca gcacaagtat
    25561 caattctttt gtatactgga aatgtttttt cttgatttaa atattcttca atttcttttt
    25621 ctttcttagc aagaatagaa ttaacttgtg atttaatctt acttacaaca gaaccagaag
    25681 aaataattga ttcgatttct tttttggaag aaagataagc aagttgtcga ttcgagaacc
    25741 caaaggtttt catttttctg ataacggaat aacccttttc aataaactca gcttctgatt
    25801 tatgaatatc ttccatttgt cttaaaaacc atggttccat tccagtgatg gatgcgattt
    25861 cttccactgt aaatccagcg ttaaacgcta gcttcaagtt aaatattctt ttatctgttg
    25921 ggactttgat cgcttttgta ataaatgctt ttttctctga atctggaact cgatgaaatt
    25981 caattaactc ttttaaatat ccatccgaac caaaaccaaa tctatcattt tcaagagagc
    26041 gtaacgcttt ttgaaaactt tctttgaaag ttctaccaat cgccattgac tccccaacgg
    26101 ctttcatttg cactccaagt gtgctatctg ctccaggaaa tttttcgaat gcaaagcgag
    26161 gaatttttgt tacaacataa tcaatagaag gctcaaagct tgctggagta actctagtga
    26221 tatcattttt aatttcatcc aaagaaagtc ctatagcaag taaggctgca atttttgcaa
    26281 tcgggaatcc agttgctttt gaagcaagag cagaagacct agatacgcgt ggattcattt
    26341 cgatcactat aatctctccg ttcttaggat tgactgcgaa ttgaatattg gatccacctg
    26401 tttctactcc aatttcacga atgatcttga gagccatatc tctcattttt tgatattctt
    26461 tatcagataa tgtttgttgc ggagctactg tgatagaatc ccctgtatgc acacccattg
    26521 gatcaatatt ttcgatagaa caaataataa cgacgttatc cgcattgtct ctcatcactt
    26581 ccaattcgta ttctttccaa ccgattacag actcttcaat tagaatctga ctaattggag
    26641 aagcatttaa tcctttcatt gcaacttctt cgaaatcttc ctctctagtg caatgaccac
    26701 cgcctgttcc agccaatgtg aacgctgggc ggataatcag tggtatcccc acttgttctt
    26761 taatttttct agcttcttcc atgttggtag caagacctga tttaggaacg ttcattccta
    26821 tcgaaaccat tacttgttta aacagatccc gatcttctgc tttcttaatc gcttcagctt
    26881 gcgcaccaat taattttacc ttataacgct ctaaaattcc ctctttcaca agactcatgg
    26941 caaggttcag tgcagtctgc ccaccaacag tcggaagaag tgcatcaggt ttttcttttt
    27001 caataatctt agtcagaacc gaaactgtaa gtggttcaat gtaggttgca tccgcaagca
    27061 ttggatcagt catgatggtt gcgggatttg aatttactaa aatggttcta agaccttttt
    27121 cttttagggc ttttgctgct tgtgttccag agtaatcaaa ctcgcacgct tgtccaatta
    27181 cgattggacc tgaaccaata attaaaacgg aagagatatc ttttctttgt ggcataacac
    27241 atgccagttt ttagaaaaac agttgaatct caagtaaata tcagttcaat ttttttaaat
    27301 taatgtgagt tagatttgac tcttgtgttc ggagtagttc gaatcattag gaaaatttgc
    27361 atcctatttc atgaggacta aagaggaatt agaggttttt ctggtattta gagccttttt
    27421 aaaaccaatt ctggaatgat ttaatccttt cttcttaggt ttaaattctt attcaatttc
    27481 tctaccatat tttttaaaaa aactgtagaa gtctgttttt agagaaaaag atcttccatt
    27541 tggatttgtc tgttttgtat tttcgcaact ataagtaaaa tttgaattat tttgttttat
    27601 aaaagttaga gcatgagaat cgagatgaat gaatttagcg accgaaaagt aatgggagag
    27661 ccaataaaga ttggtgattt tctttttctt ctttatcttt ttctctaatt tagataggta
    27721 ttttttgagt aattcaggat tttttttctc ctctgtaaac ttattcgtgt ggaatttaaa
    27781 aatccgcggg aacaacttgc cggtatattg aggatctggc tttaacgcat tataacaatt
    27841 ttgaattcta aatttataat atttaaaatc agttaaaact aactctttat ttttatcatt
    27901 atctagattc atcaatttaa attctataat atcttctatt gaaccctgct tcactacgcc
    27961 ttcatcaata aaaaaataat ataaatttga actaatcagc ccctctccac caacagtgga
    28021 ttgtaaaaaa aagaaatcct ttccatctat ttttttagat tttgtcttta atacatgttt
    28081 tatagccatt aaggattcat atagaagttt atttttttct gtatcgaata tttttacttc
    28141 agatacaaaa ttagaaatag gtttcacctg cactttgtat ttgtttcccc aattatgatc
    28201 aacggctaat aaagatttgc caataaagaa gaaaaataat ataactaaat tgaatctcaa
    28261 aaatacgcct cgatattatg atcacttacc caaagagggt atccacaggt ctcataatag
    28321 gaaagaataa cgtctgttat cttttcttct acttctaaat ccgtttggtt tgagactttt
    28381 ttctcaacgg aaataaaatt tctctctgca taaatggatt ctactagatc agtttcgaat
    28441 agttttctaa tcaaaagagg cattttattt gggagactag tttgctgaat tgcaatactc
    28501 tgaatttgtg atttcggaga gacgatattc cgatccaaat aaaaaaccaa aatggaattt
    28561 ttagagcgac ttaaaacttc taaatggata tttggataat aaaaaaatgc gtcattctct
    28621 aaatgatagt caatataagc gcctctgaga aatagttcat ctttctcatt tagaatcact
    28681 ggaaccggat attcatacaa agtctttcga atgagtttgt aatcatcaaa tccgacctga
    28741 cactgaattt tttctttttc atagacaatt tgaattttaa aggctgattt tatgccgaca
    28801 ggtcttgatt ctatatgttc gttaattttt gcgattgcat tttctgtaaa aaaaagagga
    28861 gactcattca cactaagagt ctttttctct ttaaaaatag ttttaaaaat ggaaaaaatc
    28921 ttcttcattt caattagact cagtctacct atcggctaca aattatcata ctgttataga
    28981 taagaattat tttgcttaag tttattttat tatgtccgat aatacgcgta tgagtaaact
    29041 ttattcacat attggggttg ccctttctcc agtaaaatgt gtggattgaa cacacgctgt
    29101 aagtgagggc aacatgccct acatcttttt caatggataa tacgaataag ccgctactta
    29161 ttttggattt agatgagact ttgctacatg caaatgtgga tttagtgcat aataattatt
    29221 cttatcaaac tcagtttcac tatgtctaca atcgtcccta tttaaaggaa tttctaattg
    29281 atacaaatca atcttttcaa ctctctgttt ggagttccgg ggaaaaagat tatgtagaag
    29341 agttaattga aaaatcgttt ccgagagaaa ttattctttc ctttgtttgg gatagaagtc
    29401 actgcacgca taagttcgat cgtaaactaa aggagcaagt ctatataaaa aatctaaaca
    29461 aaattacgaa agaaggtttt tccttagaac gaaccctaat cgtagatgat tctccagaaa
    29521 aatttcaact tcatcgcaaa aacggaattt ctatacagcc atttcaagga gacagcaatg
    29581 attccgaatt gctaaaactt aaacgttatc tgcaaaagat cgcaaatagc accaattttc
    29641 aaaagctaga taaagtaaat tggaatcaga ggtgagtatg gctgggttta ggaaaaaggt
    29701 attagtcctg cattcatcaa tcggtgataa tttttcaatt ttttctactt gcaatttcag
    29761 aataacaagc ctaaatacaa acatggttac tatcagtaaa cagcaaatgg ctgagtaata
    29821 ataagctcga aatatggaat agactcgttt cccaaccaaa ctacatcttc cagtggctaa
    29881 tctttattat gcgataacct tatatttaaa aatcaaagtc aaacaagttt ctaactcttc
    29941 cgagtagtaa ttcttgtctt tttttaaaat gattttggtc ccagcttttc cattgtcgaa
    30001 catctaacaa ttcccttgtt aaagaaaaag caaattcacc taaattttta tagttttttt
    30061 tcttatcatc aaaatcacga ttcaattcaa ttgtatcttt ctttccaagt gataatgtta
    30121 aattgcctaa tttatttaaa cagttatttg catcttcttc attccagtgt ttactccaat
    30181 gtaatccctt tctattccat ttttctggca ataaatgctc aatataaaaa tatcttccta
    30241 attcaatgaa tgcaacattg ttatcatgca tactgtattc tagaagtatg aggagaggtt
    30301 taatccattt agtatcataa acatctgtcg ttaaagaaat cttaagaaga tcttttatat
    30361 tatcttcttt tagtttagta tgaataaatt tttcgatttc atttattttc ttttttcctt
    30421 taatcattaa aattaattcc attactatat gctttaattt ggaaagggag acaccagaaa
    30481 tccaatagag gtagaataat tttctaatca aaaaatatat tttttcatca gggtattttt
    30541 catgaacgat tgtggttaat atggatttcc aaaagctagt ataaggcaaa taaaaaaatg
    30601 aatgaatcat attcttttca gagttatata ttcttttata tgattcagaa aatttcagaa
    30661 agtcatatac tatcgcttcc ggtttctctt ttgataattt tttgtttttt gataaagcaa
    30721 tgtatatgga agtcttcggg acattattca atttgtaata cgcgtagtat gttaataagt
    30781 tatctacatg ttcttcggta tcttcggaaa tcctttcaat atttatccag ttttgtataa
    30841 actgatcttt tttttgttta gctatttttt ccattaaata gcctttatat agatcataat
    30901 tatttatatc taatccccgt gtatttaaaa cttgaaagag ttttaccgca ttttcttcac
    30961 tataggcgat gattgtaagg atgactactt tttcatataa ataggttatg attttatcta
    31021 attgggattt cttcgaagct agaatttttt cctttagaac gagacaggca aattcataat
    31081 tggaaagttc ctttcgattg gttctctcca ttctagtaaa gtctacagat tttaatattt
    31141 ctttttcaaa atgattttgg cgatggattt gagtaaccaa tcgtaatcga aattcatcct
    31201 ctgtctgact ttttattaag tttaataaag ttctatcctt tggatataat aagtccctca
    31261 gaacacaaaa taaaattgtc agtgttgtta ggcgttgctg accatctatt actaagaatt
    31321 tattttcttt ctttattaat atcattgagc ctaaaaaata cgtatcttca ttttttgacc
    31381 atgcatcaat aatatcatcc cataattctt ctatctgctc tttttcccaa ctatacggtc
    31441 tttgataatc tggtatttcg taaaaactat caacgttgcc aaatagactt tttaaatttt
    31501 ttgcttctgg tttaaatata ttttcaaagg acatttttct ctccatactt caattgtgag
    31561 gtgaaggttt acatgtagct atcgttcgga ttcgaatttg aatttggctt aaccactttt
    31621 tcgttttaaa aatcgattcc taaatcacac cattactagc acgaaatatt ttggtgtcaa
    31681 tttttttaca aaagaaaaag ttatggatcg aagcgggcag aaggaaggca acttcccttg
    31741 cgttaacttt ccaaataaac taaaataaat ttaattaaac aatcagcggt ttggagtatg
    31801 gattcgacta ggctcaccga gcgagttata tcttctcgtg gcttttaagc ggataatctt
    31861 tgttagacgg tcttttcccg agcaggaaac aattacttca agccctattt tagaaatact
    31921 ttttacaatg gcattccaac catttcaaat tttagaaagg ctcatacata aaatgatagc
    31981 cgatttgaat aaaagagatg ttggaaaaag agaaatggat cggtgaggat aatatacaat
    32041 aggcggtatt tttggtatag atactcccta ttaaaaatct cgggagtgac atcttacatc
    32101 acttgcaaaa ttcttaaatc ggatagatgc aaacaaacaa cgcatccaat tatccaataa
    32161 cccattcaat aaaacccttc gtgtttcttc gtgcgcttcg tggtttataa tgaacccaat
    32221 cgaagtatgg ttttgaaaaa ttatatcgaa atacatggca ggtcgcgtga cctgccctac
    32281 attttttata tgaacataat tcacaaacca ccacccatcc gtgtttatcc gtgttcattc
    32341 cacccttcgc tccaagtatt cgcgaccaac gccttcgcat gtgtttaaat cgtttacccc
    32401 catgcctatc ggtgttcatc tgtgtttatc ggtggtaatc ttttaaatct cttcttcttg
    32461 tattgaattc cccatgcgac aaacggaatg gagccagatc gtgatctgcc tctacaattt
    32521 atatatgaac ataacccaaa ccacaaaacc catcggtgca tatcggtgtt catctgtgtt
    32581 tatcggtggt aatcttctaa accttcttcc caccataata attccaactg actaaatctt
    32641 caaaacttct acgttcatcg gaaacccaat caatcgtatt gcgcttaccg tcttcataaa
    32701 tataacctaa acgatagacg gccatgagtt catgtgtttc aggaactttt agaattttca
    32761 aaatcttatc ccagttttca ggaatttcca ttggagtgga tacaaactgt aaaccaattc
    32821 ctaaagaagt agcagtcagc cacatatttt gcaaagcggc gcccatcgaa atcaaagaat
    32881 aaatacctga tttttctcct ggttgatatt cttctttttt caacataaat cctagtagca
    32941 agggagaatt cgttactatc ttttttgcat ctttacctaa aatattggga actctaagaa
    33001 aattgagtag ctttactcca tcctctccaa atatttttga aatgaaaggt cttagaacca
    33061 ttggaatttt atctatgaag attccagagc gtttttcttc catttcattt tttgaaaaac
    33121 gaaaatactt taaataacgt ttaaagaaaa cacccttgga aatcaaatgc tgcatagact
    33181 ctcccgcaat gtctccaatt tcttgtctta ctttcgggtc agttactaaa ataaaatccc
    33241 aaggctgact attaaaatga gaaggcgcac gatttgccgc aaaaataatt tctcgaatgt
    33301 cctcttctcg gagtggttga tctttaaatg ctttatttgt ggttcttctt ctgattatac
    33361 tttctcttaa ttccatgttc cactcatact tctgaaatcc atgcaagtca atgattaaaa
    33421 gtttttaaat tacttgatga tttttcccta ttttagaaat ctattttcta tgaaaataca
    33481 tccaacagca attattgatc ccaaagcgga attacatgaa acagttgagg taggacctta
    33541 ctcgattatt gaaggcgatg tgcaaatcgg agaaggctct tggattgaaa gctgtgttcg
    33601 aatttattcc ggaactagaa tgggtaagtt taacaaagtc caccacactg cagtgatcgg
    33661 tggattgcca caagatatta gctttaagcc tgagactaaa acttatactc aaattggcga
    33721 ttacaacaca tttagagagg gctttactct gcatcgcgca acaaaagaag gaaaggctac
    33781 aatcattggc aataaaaatt atttcatggg tcaagcgcat atcgcacatg attgtgtatt
    33841 tggcgatgag aatatcattg ttttaaatgg acaagttgca ggtcattgcc atatcggaaa
    33901 ccatgttttt atttctggac tggttggact ccatcagttt tgtagtgtcg gggattatgc
    33961 aatgttagcc gcttgttcca aaattccacg cgatgttcct ccctatgtaa cgatagatgg
    34021 aaaccctgct accgtcattg gtttaaactc cgttgggcta aagcgaggtg gattttctgt
    34081 tgaaactaga aataaaatca aacatgccta taaagtaatt tatcattcaa agatgaatac
    34141 aacccaagca ttagaagcac tcaaagcaga aaatgaccca tcacctgaag tgcagtatat
    34201 tattcgtttt atagaaactt gcgaaagagg gatcactgat catagagaaa tatgagatta
    34261 atcgtcaccg ggggagccgg atatattgga agccatatcg tctgcgagtt aaacgctcgc
    34321 acaaaacatg aagtagtcgt tgttgacaca atggaaaaag gttctgaagt aaatcttttt
    34381 ccacaaaatg aatttatcaa aggagatata gcagatgacg ctattctcaa gaaaatattc
    34441 tcaaagaaag tagacgctgt atttcatttt gctgcgtgga agggagccgg agaatccatg
    34501 cttcttcctg agaagtattc gattaacaat ctaaatggaa ctttaaaact tcttactgct
    34561 atgtctgaaa atgattgtaa gaattttatt ttttcttctt ctgcagcagt atacggagca
    34621 cccgtttatt tgccaatcga tgaaaaacat cctttgaacc aagaaaacta ttatggattt
    34681 acaaagtatt ctattgaaga aaatttaaga tggtatgaca aactaaaagg gatcaagtat
    34741 gcggctttga gatattttaa cgctgctggt tatgacacgc aaggtagagt tagaggtctt
    34801 gaaagaactc cagcgaacct tttacccatt atcatggaaa cggcatgcgg catgcgacca
    34861 agttttgaaa tttttggtga agactatgat acaccagatg gaacttgtat tcgagattat
    34921 attcatgtca gtgatctcgc tctcgcgcac cttctgagtt tagattatat tctcgaaaag
    34981 aaagagtcga ttacaattaa tctaggctct gaaaatggat actctgtaaa agagatcact
    35041 tcgatggcgg aacaagttgt aggaagacca attccgcata agattggtcc tcgtagacca
    35101 ggagatccag caaaactttt agcgtcatcc gctaaagcta gagaattact caattggaag
    35161 ccagtcttta gtgattctaa aactttaatt gaatctatgt ggaatgttta cccgaaaggt
    35221 taattaacgt tagtcagttc gatgtaacag aatattttaa agattgccac ctattgccgc
    35281 gaacgagcgg caatggattt tgggtggaaa gataatgggc accgataaaa gatatgataa
    35341 ttcaaaatta tccatcgctt tttatcgttc agttatcggt gtcctcggtg gtaatcgttt
    35401 atattcatct gtgttaatcc gtggtaaaaa aaaagtgagt ctactaaact cacttttctt
    35461 cgacaaacag ttattaaccg cttgcttgat cttcgtcttt ataagctgct agttcttctt
    35521 gatttggact cacttcttct tcagacttag tcattgagta gccgccttga ccaacctcaa
    35581 ctatattaat atcttcatta gaaggaatcg gaacccctga tggaggaagt aatgtaaaag
    35641 aatgctttcc agtttgagtg aacggaggca tagtaaatgt tatttccgaa gcagatgtgc
    35701 attctgcttg aattggagtg ctaaaaccct caaccataac cttcatacca gttaaaaatc
    35761 cagacccaag aacagagacc tttacattct cttggttaat ttcaatgtta tttggagtta
    35821 cagcattgat ggtaatttct ttaccgatag ctttacttcc taaatatcct gtttggctaa
    35881 cacccattaa tcccaataat gtaggaggga tatccggtag tccctgcaat ggattcccaa
    35941 gtgctaggtt ataaatatac gcacctattc caacaatagt aaatccaaag agttgcagac
    36001 gagataaatc aacacttccc ccttcacaaa agagattact taattcaggt ggtttacttt
    36061 ttatttcatt ctttggtttc tttgccccta agccaccagc agcaatcaat ccaccataac
    36121 taatagacat taatataatt aaattaggat taaaatctgg aatctttccg ttttgtagca
    36181 ataatccttg gctgataata acataagagt aactaccgat agcagctatc gtccagacaa
    36241 aagcttgaaa cttagaaaga ctataactat tcgttgcctt atcagtcaga ataagctcaa
    36301 agaaatgaac tatagtaggt cttttctctc ctccttttct ggctatctct ctctcctgtt
    36361 gtctcttaat caatcctctc aatatcgtat ataggaaaat taaaaataga acgatcacaa
    36421 ctataccaaa caaagcagaa tagaatttcc atgtaggagg taataaggtg agacccacaa
    36481 aattagaagg tctaccagca acaaccattc taatttttaa agactttcga attccgccct
    36541 tcattaaatc ttttttaaga ggaactgtaa atctaagttc ctgtgattca ttcacgatag
    36601 gcgacaagaa aaatggcctc ttctcatcta attcagtata agtatcttct cctgtattgc
    36661 taacgattga ttcatagaat acaatgctca ccttgtctat atcagatcca aaattatttc
    36721 cataaacagt gattgtatcc cctcttgtgc ctccttccgg gctgatcttc tctatataag
    36781 gttgttttcc aggctgatca tttaaggggg gaatttcaaa ttcaagttcc gactcttgtg
    36841 tttcacttgc atttggatat ttagagaaaa gttttattct tctttttcct gcttcgattt
    36901 tatagtaagc cgccacaaca aaattaataa cgtttggatt atcagcatcc ttctcagcgc
    36961 tatcaatcac gatatctgat gggatttctc caatctgaat tctaagcttt ttatcttttt
    37021 taccatttaa ctcttcaatc agttcatccg gtctatcagt aacaatagaa acccaatact
    37081 ggtctgaaga taagtcctta aagctaaaat acttgatttc atatttagaa ctagatgaaa
    37141 caggtgcctc agtcgtctga ttgtttgtag ttacaggcgg ggcatttgtt tctgttggag
    37201 tattaccaat ctgtgcaaac agaatggatg gaaggaatag aaataaaaaa atcttgtaga
    37261 gcaaaaggaa tctccctaaa aattaagtca gttttacagt tttaattatc gatcaaaatg
    37321 taaattaaaa tctaaaatta tacttcacat acgagtcgta agacgtgttt gtttgtattg
    37381 cagatggact caccggagtt ggcggaaaaa aagcaaactc aaaggcatca ttatcaaccc
    37441 ctagataata aaataatcca gctaaagcaa gcatcgcaaa aactcctccc gcactggcag
    37501 ttctgtaagt tgtaaactgc ctagcagctc tctcgttact ttgaactatc gtattttgat
    37561 aattataaaa tataagaagg ttggttatag aattcgaatt actaccagag aaaaaacttt
    37621 gataagttgt aaataaagtc aacccccctg cgacttctcc ttgcaatgga tctcctctaa
    37681 ttttatctct ttgcacacga gctcggtaat cccaatacat aaaactcgca taaaataagc
    37741 ctaccgaata aacagaaaat tgggataaat caaaataact ataatcaaga aagactttca
    37801 acctatgttt atagtaatct tctgtttttc cctttttcaa ttttgcattt actgttgtcg
    37861 tttttgaaga atcaacttct actccagcaa aataatctac atactcttcc ttttctatac
    37921 gaattcgatt ctgacctaaa ggaaccacta cgttctgaag aggtgtttct cctagatatg
    37981 tcattcctag atagacactt gcgcctgacg gttcagattc aactgaaatc aatccttttt
    38041 tttcgatttt cttcaattga aaagaatact tagaaacaga atcctttttc aaattcacta
    38101 ctcgggagag agtttcgtgt ccttctttat tgattgaaat tgaatgttga ccagaagcta
    38161 cgtccgtctt ctcgagagga gtctttcctg cataatgtcc atcaataaaa acaagtgcgt
    38221 ctacttcctc acctgtctca actaggatcg ttgccatttc tttgttgaat aaaagctttt
    38281 ttagtttgaa acccatagag ctcatttctt gataagcacg acgaatggaa actgtttcta
    38341 caatttgatc tactttacca ttttttcgat ttgtaaactc taaggtaata gacatactat
    38401 cgttttctct ggttgaatat tctccagtta aaacatagaa acatttattc tttctcccaa
    38461 tctccaaatt ttcttctaac ataggagggg tttccaattc aataaacttc acttccaatt
    38521 ttatatagcg cggatctttt tccggtaaag tttctttttg acctgaattc aagtcactta
    38581 tagttttacg gttaaatgtt tttttacttt ctttcgctgg gagactcttt gcattttctc
    38641 catagccatg gtaaataacg gtttccaata catcaggatc aaagacaaat ttaaagccat
    38701 tcagatttga aataactaca gaagggattc catttgagag atattgaaac ttctcatttt
    38761 cattcttatt tctaaatgga aatacgcaaa gttttctaga tttttcatat tcaatttttt
    38821 caggaagaga ctgatctaaa aaattaaaat aatcatctac tccaaaaagg gatggaatca
    38881 aaataaaaaa taatagaata ctttttggaa aaaaatttag aaaaagctta tttcgaattc
    38941 gactcatttg acttcttctt tgaataatag atgtcataag accaagctac ctacattgat
    39001 aaatttctag ctaccagatt gcgaacaacc atttcatccg cttactgagc gagcgcctat
    39061 agtgaattat accaaacact actccaataa aaccattgag ggaagtttct ttttagggtt
    39121 tggtatatat tgactatctc ttattacata cttttttttc aaagtcgaaa aggtttttta
    39181 cttcctcttt ttactaaaag ataacactaa aaatagaatt gcaatgatag gtgtaaacaa
    39241 agctagagga gattttaaaa aataatcctt tgaaaaagca atttgctctc ccagaccagg
    39301 accaagaaaa tctgttccaa aatctattcc catataacta aaaagggcaa cagtcataat
    39361 tacactcgga atcccagtaa taaatagaat atagagcaaa cttgatattt ctggcaatat
    39421 atgagttcta tataaataaa agtttcctcc acccatgatt ctagcagcaa ttacataacc
    39481 actttgtttt acttctctaa cctttgcttg aatagactca aatgcaaatg cccaatcgga
    39541 taaaaagata gcgatataaa atacaactaa ttcaggacca agagaataaa gaacgataat
    39601 tgccacaaga aaggatggga gagagagaaa aacagatgaa atagactcta acaataaatt
    39661 tgaataccta ccagagacat aggacaaaaa ggagaccatt accgacaaaa aaagagtgat
    39721 gattctagca ggaatcgaaa ttaaaaaagt cgtgaatgtt ccatacgcgt ataacgcata
    39781 aatatctcta cctaattgat cacatcccaa tggatgcatc cagttctcaa agggagggaa
    39841 tcctgaaagc gttaaatcaa ccttaactgg agccggaaaa aataacatac caatcaaaat
    39901 agatacacta aaaataattt ttaagaaaac catattctta atctaaagcc aatttatact
    39961 gtaataactt tgcaaacctt gtaagagaat aaaaaattat acctgaataa aacaaaagag
    40021 cccttaacaa attttcatct aaactcttta ctgcataata aagagatttt ccaatacctg
    40081 gaaaaaagaa aatttcttct actaaaatgg aaccagaaag caaggaacta aaatcaatca
    40141 atataaagat taaaagaagt ggtagtattt ttctaagtat gtaataaaaa acaattctag
    40201 agcgactaaa tcctcttgtc tgcaaaaaga aaaaatactc tgagcttgtt tcttttttac
    40261 cttcctctaa gctaatgtag aaaattcggg caaagaccct agatcctaag gctgtagcag
    40321 gcaggatcaa ataaagaacg ttattcgcct tatatccgcc aggtggtaaa actcctagat
    40381 acaaaaagaa aaccaaaaag agtaaaatgg aaacaacaaa gataggagtt gatagtatca
    40441 atttacacaa aaacaatata aacatttcta ataaatcgaa ctgtaaataa atacaaaaaa
    40501 gtgctaaaaa taaagcaaga atagatccac ctatggatgc caaaatagaa agctgtaaag
    40561 taggcactac tttctttaga atatgagaat ctacattttc accagattct gtttttccaa
    40621 aatccaagag gataagagac ttcatggaaa ctagaaaaga atttagataa ctcctctcag
    40681 tggctaactc ttgtatatcg ctattttcaa gactagactc tgcatataaa aaagtcttat
    40741 cctgagagcg aatggttgta atgattgaga taaaaacagt caggcaaaga gtgaatagaa
    40801 taaattgaaa aaactctttt accaaaaact caaaccttct tattgagaaa aggctgccaa
    40861 aatatcatcg taagagtttg agcaaatcat tttgttgcgt tcttctttta ggtttagagt
    40921 cttagcgatg agcgcgagag ttttgatatg ttcttgaaat ttattcttag ggactattag
    40981 taatataaaa atgtgaactg gcattttatc aattgcatcg aacgcaatgc cttctttaga
    41041 taaaccgatt acaactttca aatcctcaac aaggcttaca gaacaatggg gtatagccac
    41101 tccgcttcct atgcctgtag acattgactt ttctcgattc agaagagatg taataatttc
    41161 ctgactagca ctgttcgcta tgctacctat tttcacaccg tgctctacaa gacttgcaat
    41221 cacactttct ttatctttag cctttagatc ataaatgata ttattaggag ttattagttc
    41281 taataagtga ttcattttct tataatatat ctatagagta agtttttcgc gaaaatactt
    41341 tctgtcacta aataaaaagt gaatccatat aaaatgaaaa aaattattat aaaaagaatc
    41401 ggaggaaata ttaagccgga ctgaatcaag aaccaaccag ccgccaaagc caaaacacca
    41461 acaataagcg aaataaagga agataaatgg tgacttcttc ctggtgcgga aaacaaaata
    41521 taccacaaac tactaaagtt tattatttta tttttacaga gaagtatatc gtatctataa
    41581 taaaaactaa acagaaaaac aaaaaccgaa atcaaaaaaa gatctaataa gaaattttcc
    41641 tgtcttaaga aaacaaaacc cactaaaatt aataggtata aatctagact ttccaagact
    41701 gaaaatgata aatttagaaa cgaacttcca ggaatactca ttggcgtaag aaacaaccca
    41761 ctgataccag aataaaaaga taacataggt atttctgagt ttgatgttgc taaattcatt
    41821 ttgagaatat ggaaatgatc taaaaataaa gcagtggaga cagtaagcaa agataaaaat
    41881 aatccagcag gaaatctaaa cttataaaat aaatttcctt ttaaagagaa taagaaatag
    41941 ataccaagac ttatactcag agtaaaccaa tttaaaaaac ctaaaggggt aatcagaaga
    42001 tagagaagca tctgataaaa aaagcctata taataaatat aaccgattct cagtttataa
    42061 aaaatataaa ataaactgga ggatattagt aaaacaatca aatagaaagc aaatgataaa
    42121 ttctgcaaag ctacaaagat agatattcca tataaaacca aaaatgaaaa tatatccaca
    42181 aagaaatcgt ctgagaaaac tttgaagcca cggtttgtaa gtagtatact atgggatgac
    42241 aaaactagct ccttgtttta tttcggctat acgcgtagat aaatcgatat gcgatggaca
    42301 cagtaaggaa caaattccac aatcaagaca aacacttgaa ttaaaacctg ttttatcttt
    42361 gtctaaaatt gctcttggat ttgcagcaac aggacaataa taagaacaat cattacaatc
    42421 ggtgcaagac ttttcatttt tttcaaaatg aattctatcg caaatgatca cagaatgatg
    42481 tttgtaaata tcaaaaataa attctgttcc aatttcatag actggctgct tatcataaaa
    42541 cgaatttaca gtaaaatatt tatatctagt ttgaaaacta tttaaaaaat cagatagatt
    42601 taatccattt tttaacaaaa atgctttagt ctctccctct aacgttccgc tttgattgat
    42661 aatattgact gaaacataac gttcatgaaa tgggatatta aaataaagtc cacgaatcag
    42721 atgataaatt gtttcaggac ctaaatacaa aaatctatct ttaggaaagt cctttgcaat
    42781 atctatccca acatatttat gtaaaaaata tctaggacta ccttctggat agatataatt
    42841 cattttcttg tctgtaagaa aatcaataac gttcacattt ggaaaaattt cttttatgtt
    42901 cttttttaat ttttctaatt catttttata aacttcaaaa atcttacttc tgtagtcgat
    42961 taggttttct tttgtaaatg gaacgaataa aataaaggac tctctgtctg gaatcaaaga
    43021 ggaaaataat ccagataaag attgtcctgg aaaatctaga gaaacaagac cggtagagtc
    43081 aatcttctgt tttagtgatt ctagggaata attaccgtta aacgcttgag gtttaaaatt
    43141 caactcacca tcaattgtaa atcgaattgt ggaagtactt gggtaataat gtgctatacc
    43201 attagcgggc gaaagaatac gagcagactc atcttgaaaa attatatccc caatagaaag
    43261 acgcctggga aactctccaa caggcgttag cttcctgccg taaaactcac cctgaatgtt
    43321 tagctcagat agagatggga caggaactct aagatctgta gaacttttaa ttttaaagaa
    43381 ttgagaagtt ttgtaggttg ccaagatgaa cttcccagac tacttcttta agaagcagtc
    43441 tttaccatat aaatttcatc tttgataggt agttcttttt ttagattttt gatataaggt
    43501 aagatttcac ccatttcttc atagaattca caatcagctt gcatacgtct tgctacagta
    43561 aaatacttat aaagcttttc ttcgccggat cttttagacc attttttctt agtgcatttt
    43621 actcggagaa tatatttatc ttgttcagct tcaaaatttc ttactacaac acagttaatg
    43681 caattcgcac aatagacttt ctcgctcata ctttgagtgc cacctttctc ttggttttaa
    43741 ttatttcaga gttgatagat tatcaactct gaatcagtaa atttagtaat cagtacactg
    43801 tcaagcaatt tcgacattga ctgcaggctt ctcttgcata gatggaagac tccaaagagc
    43861 ctctttgatt gaattgatcg caacaaagaa aaatccaaga gaatagataa acataaatcc
    43921 aatgataaat ggtttttcta caacgatgga aacatatacg cagaataaac aatatactcc
    43981 cataaaaaac tcaacaagag ttaataaatc aaatggaacg tagtatttta gtctttcttt
    44041 gatattgtca gaattttttt ctaatctcat ctttggagtt cttttaaaac tagattggat
    44101 tccaagaaca gcttctagcc atgctcttgt atttacaaca gcaacgccag ttccaatcat
    44161 aatcataaca ggtaaaaaga aaattctaga tttccaatct gggtagattt ctctttgaga
    44221 atacatataa aatatgatcg gtccaatcga tccaatcgat agaaaggcag ccgtagcata
    44281 caagatcaca acgggtagat caaataaatt aagcccagac caaaactcca taagtaagag
    44341 aggcgctgta aataagatat taatgatcat aagcggatga acggaatagt taatcaagtg
    44401 aacgatcgct tcccctttta tcttccaact taaattagca tcccaaattc taggtaatag
    44461 tttcacagcc gtttggatac tacctttaca ccatctgaat tgttgagatt tataagcgga
    44521 aatcatagct ggaatttcag ctttacaaac gatatctttg aaataacgga acttccaacc
    44581 ttttaactca gcacggtaag aaagatcaaa atcctctgtt aatgtatcat gctcccaacc
    44641 accagcatca attacacaag actttctcca aatcccagca gttccattga agttcatcca
    44701 aaggttacta gcatttctag caacttgttc gatcataaag tgaccgtcga ttccatagct
    44761 ttgtgcttta gtaagaagat tatagtcttg gttgatatga ccccatcttg cttgcaccat
    44821 tccaatttct gggtctttaa aatatggaac tgtttttagt aaaaagtctg gttccggaac
    44881 aaagtctgca tcaaaaatgg ctacgaactc accctttgcc ttagcaaagc cagcttctaa
    44941 agctcctgct ttgtgtcctg cgcggttcgt tctatgtaaa tgctcaatcg caatgccttt
    45001 tgcttggtat tcttttacaa tacgacgaac tttctcaact gtttcatctg ttgagtcatc
    45061 tagaacttga atctctaaac gatttgcggg atattgtaaa cgaactgccg catcgattaa
    45121 acgatccact acataaaatt cgttgtagat aggcaattga actgttacaa agggaacatt
    45181 tgaatctgtg actttaaaag ccagatttgg gtcggattcg cagtaagatt tgtttttctt
    45241 atagagccat accattaaaa gagtatgaag actgaacaaa aatagaacaa aaatgtctac
    45301 tgcataaatg gaaataaaaa gagcggttat aataattagc atacttgtca aaatatagac
    45361 tttaggcgcc ctgtcaagcc aaatcccttt cctagttaga aaccaccctt cgaaccatgc
    45421 tttcaagtca agggagattc tcgaagatta ccacctatgc cggcgaaaat tcgagtatgg
    45481 attttgagtg gaaggacgat gaacgatctg actaagcttt ccgcatcgct cagataaaag
    45541 atacgataat tcgaatttat ccatcacttt catatcgttc agttatcggt gccctcggtg
    45601 gtaatcttct cagggaataa tttcgaaaat tggaaatttt tttcttgcca ataggagaat
    45661 ttatgtattt gtgaaacccc taaaggatgc cgatacacat tatggaacaa aagaaaagta
    45721 aatttatgtc gcttatagag agaattacta caaaagcaga gaagctagat caactttgca
    45781 atatcattcc acaagagctg gactataagc gtcatgagcg cagtaaatgg aatagttggc
    45841 attctgaatg gaaaaaagct tggattgact cctctgaatc atacctctca gacgaagaaa
    45901 gagaagcaaa acgattagaa acttctatta ccactcgata cagaactgta gactagtttt
    45961 tcaatttcct ttgttctaac cttctgatta aaaacacaaa gttgaaggag atttacccag
    46021 acaatggaaa catcattaaa cgtaagaaac atcattgcag caaatttacc aaaggaaaat
    46081 ataattccgc aagttgaccg tgacttggag aatttaaaac aaaaagaaga agtaaatcaa
    46141 aacgaagatt cttttaccaa aaactttcaa aaagacagca aaaccacttt agaggaaagg
    46201 ctaagtcaag ttattagtgc agatgaaatg aaaaatttac tttctatgct tatgagaata
    46261 ccaaatgaaa caactatgaa tagtagaatc gatttaagag tctgaggagt cccttctgtt
    46321 agcgattatc atacaagaca gttccttttg gttattgggg attctattta tcatttgttt
    46381 aattggactt ggtgcggtat tttatagcca acttgttcaa ccctttatcg atcaaagaat
    46441 ttcagaaata gaataccctc ccgaaattgg tcagaaattt gaatacattg taaatgaggc
    46501 agatagagtt tcctattttt caattggagc aaatacagga gacattccaa ctaaatgtgc
    46561 agctatttca gaagatcatc taaattttat attcaagaaa gaccctcgaa acgaagaata
    46621 ttctataaca atcaaaagat ctggacctgt attgtataaa catcctcgta tgtcccactt
    46681 tctcaaaatg gctgggacag aaaagttaga aagtcacgaa ttgattggaa aagtagcaga
    46741 gtttagactt tcagataaaa tttcaaaaga gcgtatgatt gacttcatcg aaattacttt
    46801 gtctacagcc tttgtaataa gtaaatctgg aagagaaaga atgaagttta tttttacctt
    46861 caacaaagtg catccaggat ttaatctaaa aacaaaaaat agaaagggac tctttccttt
    46921 aggaaaagat agtcaccata aagaagatgt tactccaacg gatgaggata cttaagatta
    46981 gcctttcttt tttataatta tacaagctag gtattctaac gtatttccaa ttccttgcga
    47041 ctttccgaat ccatccaaaa aaagacttag acgaagcatc aattccttag agtttttaaa
    47101 agtaggcttc gaaaacactt cactttctat cattgatacc aaatcacttg gattgatatt
    47161 taggatttca tgaacacgat ctgagagtag aattagatac gaaggattaa ccaaaatcgt
    47221 tttagaaaaa gttgtatcct ttcttttaaa atcaaaatat atttcactac tcacgttagt
    47281 agaaagacca ttgtcactta aaatcatcat cgctgttttt tggttttgta aaaagctaat
    47341 atgatttgag tgattcgaaa cgaagactaa acttaagttt aaattttctt tttccgttga
    47401 ccgaatcgca ttccatatca gatataaaat ttcttcctct tttgcattgt atagaccgga
    47461 acttagagca aataatccct gtaatttata tttgtagatt gaatccaata tttcaatttc
    47521 ttcaaagcct gccattacgc ataaatatcc caattcaaaa tcaatcacag tcacaaaatc
    47581 acaccgcgga ttattagtga aatttgggaa aattgaaaca tctagattct ccaaatcacc
    47641 caattttgac tctggcaaaa atggctttaa tcgttctacg atacttgtat tatttaattc
    47701 tattgctttt tttaagcggt cttcttctct ctgtttactt aaaagaattc cctgattaaa
    47761 aaattgaatg atttgattta cttctgaatc tgggtagata gaaatacccc tatcttcatt
    47821 aggaactaac gtcttactcc aatattggat gagatgagaa agatgacgaa tcgattcata
    47881 aaaattattt gtaaaaacat aagcgcccag acctgctaat atcgaaaacg aactagcaat
    47941 caaaagtcta gcccagaaaa aatttcttgg aaaaacttct ggatttgtct catcgatctt
    48001 gctaagccat agagattgtt caattatgaa gaaaaataaa aaagcagata aaactaaaat
    48061 aaaaactacg atagaaactc ttaatctaaa actcagtgtt tgcttcatgc ccttctcgca
    48121 acaactatca ttctagatcc atggttttta tacttttcca ttttataatt tccatagatt
    48181 tgtattattt caaatttatt gttttttaaa atagaggaaa attcagatac agtgaaaact
    48241 cgcattatat gtttgtcttt gatttctttt ttatctaaat tatagacaaa gtttacttcc
    48301 actagagttt cgtttaagtc atctatacct tcggattttt ttaatttaaa accacgattt
    48361 cttttaatta ccatatttcc aacttttgca tgaccaaccg ttgttaatgg ctttctttga
    48421 atgaggcgaa gcggaacagc gttccatact tctaaaacta ataatcctaa gggcttcaga
    48481 ctttttcgga tattatccaa actggattca atttcttgat ttgttagtag atagttgatc
    48541 gttccaaata tgcagtaaat tccatcaaag aatttttctt ttttataggt ttgcaaatct
    48601 ccaacttcaa acttacattg aggaaaccgc ttaattgcct gttcaatcat cgctttagag
    48661 gaatcagttc caattatttt ataccccaat ccttgtaagg cactcacatg ctctcctgtc
    48721 ccacatccta tatctagaac agtctgcact ttgtgttttc tataaatttc atctaaaaag
    48781 gaaacttctt cgttaaatct ccgcccgggt ttttcaattt cgtagtagta ttctgctaat
    48841 tcagtataaa gcttcatttt ttaatcttat tattcatcta agtagtgcga aaatatattg
    48901 aattgggttc ctatttgccg ttaataaaat taactcttaa aagtaattta caattttctt
    48961 ctctcaaggt aatcggtaga agacttttac attctaaacg ttttggaact tacttttatt
    49021 gattggctat aattttatga caaaatattt tttcctaatc ttctttttct taatactcac
    49081 cgctgtcgat gctgataccg caaagagagc atcctcacaa acagacgtat acatatgggg
    49141 cagtatcttt ttagccttat caatgggaag ccttttactt gttgtaaatt tatttccaaa
    49201 aggagctgct tacaaagcat acgaagcacg tcttcaacat cttagaaata atcctgcagc
    49261 caaacctaaa tcttccaatg aggaaaatgc tcctggtcaa aacactgaag caaataattt
    49321 acaaacagag attgaaaaaa caaatccgca gacaaagcca gccaacgcaa ctgctccagt
    49381 catgcaatcg acacatacaa gtgtaaaggt tccagaaaca aaaccaagta ttcctgccat
    49441 caatccagtt ccacaacagg aatgtgtaat cattaactat aataaagtct ttgagtcccc
    49501 ctcagcgcag ccagagactt cctgtaagga tttcgtgaac aagctttcca atataatcaa
    49561 gtgtaaatct atctccatct actttgttca caatcaggta ttcagctgtt ttatagagaa
    49621 gaaagctgat atttttacta aaatggaagc aggaaagaaa tccgatttat cagacgaaat
    49681 cttaaagttt ctaaaaaata aattaggggc attctcttct aatcatgcag atgcagtgtt
    49741 gccactcgta acaaacaatc aattatttgg tgcagtaaaa ttagaatttg cagaatcact
    49801 agctaattta gatataaatc caatttggtc agaagtaaaa gcatttgcga aatactttga
    49861 tcagtctatt cattacaatc tttcagttca ggacaacgaa acttctctct ttacattaga
    49921 acactttaat aacattttaa actacagagt ttcacttgat ctgccacaaa acctatctct
    49981 catcaaaata ttaaatacaa cagacaaagt aaaaacattc aaagatatct ctggttgtgt
    50041 taaagaggta attggcaaga aaccagaaat atataaacta tccgatgact tgcttggaat
    50101 ctttttgacc attgaagata gagacaaagt tagcaaatca atgggaacac ttgtcaattt
    50161 gttaaaaaag aatattgtaa atatagaaat tgcaataggt tccgcagact acaatgaaaa
    50221 tataaaattt gccaacaaat ggcatgagag agctaccaac gcactcaact cagcgattca
    50281 agcaggaaaa aataactttc gactctttgt agaaccagaa attaaatctt aatctatgct
    50341 ggattataaa aggattctac taggactacc tttcctagtt cgttctggcg gagttaaatc
    50401 ttctagaatt gtaaaaacaa gggaaaatta tttccctagt aaattagttc ctggtaaaaa
    50461 gtttagattc aaattttttt attgtgatga atttattcag gaaaatagac ccacaatcct
    50521 tctaacgcat ggaatgagtg gttatggaat agatgatatt agaatgacag aggctgccac
    50581 aaatttaact gtatgcggct atacagtagt ggttcctgaa ttcgaagaaa tcaaagcatt
    50641 aaaaatttca caggaaagtc ctagaaacat caaagatgca tttctaatta ttttggatca
    50701 aaaagattta aatacagaat ctattggttt tttttccatt agtttttctg caggctttgg
    50761 gctgattgct tttacagatc cagaaatttc cacaaaggta aaatcgatta ttgcgattgg
    50821 cggctttgca gaaatcctag acacttgtaa tttcgcaatg ctaaactatg aaaaagacga
    50881 ctatccaaca aatatactct tttataatta tatccattta ctctatgata attgcgagga
    50941 actagccaag gctttttatg aggctgcact tgacaatggt ctatcccgta gaggcaaaga
    51001 ccaagttgcc cctcgaatct atgaaaattt aagtaaggga catagagaaa tttttgatag
    51061 agtaaaaacg agtgcaacct ttcgcaaaga attgggacta aaaataagcc aagcctatga
    51121 aaaagaagca aaatcaattt ctcccattta tttcattgaa aaaatcaaag cacctgtttg
    51181 tcttttacat gggaagaatg atgatgtcat ttcagaaaat gagtctataa aattatcaaa
    51241 gcaaatgaat gcatataata ttgatcatgt tttagagctt acagggttat tatcacatgg
    51301 agataagatt cctttttgga agcaactgag tagtttacct ggtctttctc atgcatttgg
    51361 atattttttt gaaaatttaa gggaaaagaa ataaatttcc tttcaaatcc gataattaaa
    51421 tataagattg tggttaaaga aaatagagtg acaaaaaatg gataaatgat atctattaca
    51481 tcctgcaaat catctattta taaagaaagt tatggaagaa agaactcaaa ataatatggc
    51541 ggatatggtg caattgtctg agactagact tgattgcatt tcaattgaaa tagcaaaaga
    51601 cgcaatgtcg gcttatctca ttgttgagat aaatggtgta agtatgagag atttctcccc
    51661 tactatgctc tacaatgcta tcacaaactc aaagcttagt gaagaggttg taaacttcag
    51721 tttaataaaa gaagtagtcg atgaagttac aaaaagaaaa aaacaagttt taccgattaa
    51781 tcaaattcaa gatccaaatg aattaatagt cagacaacag atcgctacag gaactccagt
    51841 cgtgcctggt gaagatggtt gggttaaatt ctatcatcca cacaatcaaa gagttgtaat
    51901 caaacaagat gggtcagcag actttcgaaa tttagaaaaa tttatttcca tcaaaaaagg
    51961 agaaaaaata gctactctat ttgctggtgt tcctggcaaa cctggcaagg atgtttatgg
    52021 aggagtttta aatgcagcag ccataaaacg cccaaagatt attataggtg caaacatcat
    52081 tacaactaac gtttcctcac ccgaggaacc agaaaaagtt tttatagact atacctctgc
    52141 ctgtgatgga gttttatttt ctacagatga atcagtaacc gtttctccgg aattaagaat
    52201 tgaacagagt gttggtattg caacaggaaa cgttaaatac aacggaactg tcaatgttat
    52261 tggttccata gaggatggag caaaagttga atgcgaaggt agcctgaatg tagctgaaaa
    52321 tctggagtca tccgatattc atgttgggaa agacctaatt gtaaaaggtg gaatcaaagc
    52381 aaaagcgaaa ggattagtaa aagtagtagg caacgtcaga gctaaatttg ttgagaattc
    52441 cattttagaa gtcggagggg atgtaatcat tgagggatat attctgaatt ctaaaatcta
    52501 ctgtttgggt agcattatcc taacaggagc aacaagcgca atcctcggct cagaaatcat
    52561 tgttatgggt ggactatcca cttacaatct tggttcaaat gccggtgtcg atgtcagcgt
    52621 agaactagga gttcattttc aaaatgaaag actttttgat gaatttcaaa acaaagtaaa
    52681 agctgctgac aaagagatgg cgaatctaat cccgcaagtg caacaagcaa ataatatgat
    52741 taagcagttg cgaggaaaac tagatgaaga gaaaaagaaa aagttcactg agcttttcga
    52801 attattcaaa caaaagagtg caaaccataa atttgtgact gaaaaatttg aagaattgaa
    52861 aacatctcgt ttcaaccaag aacatatcaa tatagttgta aaaggagcag cgttcccagg
    52921 ggcagtaatc aaatatagaa ggcaagttga aaaagtaaca acaatgcaat ctgcttttat
    52981 gatgagcttt actcctggac aggaaagcgc tcctatgact gctatcaatg tcaaaaagaa
    53041 gtaatcttta aattttttac aaaaataaaa aaatcttttt gaaagacttg gtacgtcttc
    53101 ataaactggc aataaaattt attcattaat agggattcca tatgcaaata aaaaagaagt
    53161 taagcgtatt gatcggatta cttttagtaa tttccttaaa taccagttac attttttcgc
    53221 aagatacaaa tggtcctaca acaactccta caacggaaca acactccaat gagcacaact
    53281 caggtggaca tgatgaacac aaaggtgctg atttagcgct ttggtctgtt ataccgtttg
    53341 tggttatttt gttatgtatt gccattcttc cgatatctcc tcatcatata gcacattggt
    53401 gggaacaaaa taacaataag ctactcattt ccggaatttt aggcggtata gcttttggta
    53461 ttttaacagc aaatggatgg attggaaaaa tttatcatac gttagtcttt gagtatgtcc
    53521 cttttattat cctacttgga tctttatttt atatttccgg tggaatcgtc ctaaaagggg
    53581 atataaaagc aaccccagta aataacctac ttttcctttt agtgggtgcc tttttagcat
    53641 cgttaattgg aacaactggt gcctctatgc tactcattcg accattactt aaaacaaact
    53701 cagaaagaaa gcatgttgtt cacacagttg tatttttcat ttttatggtt tcaaacattg
    53761 gtggatcgtt aactccactc ggagatcctc ctctctttct aggatattta caaggtgttc
    53821 catttacttg gactttctct ttattccctg aaatgattct ttcaattgta gtactatcaa
    53881 tcgtttatat aatttgggat aatattcagt ataaaaaaga gaccaaagtt gatatcaaaa
    53941 aagatatcac aaaagcagaa ccaatttcta tagaaggaca agtgaatttt ctctggttac
    54001 ttggtgttgt tttatcagtc gcttttttaa atgaaaatta cgttcctgca atcaaacaca
    54061 atccatatat agcattcata agagaaggag tcatgattct tctgatactt gcttcttact
    54121 atacatcaaa tccaaaatta agagagcata ataagtttac gttaggtcca atccaggaag
    54181 ttgcttattt atttattgga atttttgtaa ctatgattcc tgctttaatt cttttagaat
    54241 ctcatggaaa agaattaggt gtaactgaac cttggcaatt tttctgggcg gcaggtggat
    54301 tctcttctgt attagataat gcaccgactt acctaacgtt tttaagtctt gctaaaggac
    54361 tcactggagc gatggatgtg catcaaatac tagcaaatcc agaatgggag aaactactca
    54421 aagcaatctc agttggagca gtatttatgg gagcaaatac ctatatcgga aatgcgccta
    54481 actttatggt aaaatctgtt gccgaagaaa acaaagtaaa catgccaagc tttggtggtt
    54541 acttattgta ttcttttgga gttttaatac ctttatttat cttgattact tttattttct
    54601 ttaagtagtc tatacatcgg aagaagagcg cataataaat attatcctct tcttccacaa
    54661 agttcacaca ttcgtttctc cttcctcaca atattcatca aatgtttcaa attcaattgt
    54721 tgaatgatga atgtttgcat gggctaagat atgtcgaata ctctgtttta ttctcaataa
    54781 gtcttcaatg tcagtattct tttttacacg aacatgacct gtaaatgctg ttaatgttgt
    54841 gctaattgcc cagacatgaa tatgatgaac ctctaaaact ccatccactt tttcgatttc
    54901 tcctttaagg atcaccatat cgattccttc aggaactgca tctatagaca aatgaaagct
    54961 ctttctaagt aggtcaatac tactatacaa aacaaaacaa gctattccaa tactaattgc
    55021 tccatctaac caataccaac tcgtaactga aatcaatatc ccagctatta atacaccgaa
    55081 ggaaactaac gcatctgcaa tcatatgcaa ataagcgcct ttcacattta agtctttctc
    55141 tttatctcgt aaaaataacc aagcagttaa agaatttata ataatcccta tcgcagcaac
    55201 aatcgcaatt ggatttccat ctacggaatt cattaccttg attctatgaa tgctttcata
    55261 aataattgca cccatcgcaa agacgagtat aatagcatta acaagtgaaa ctaaaatagt
    55321 ggtttttcta tacccatatg tgtatttaac gtctggtttt atctttgcca atcgaaatgc
    55381 aaacaaagcc aataatagac tagctacatc gcttagatta tgagcagagt cagaaaggag
    55441 cgcaagcgaa tcaaaataaa atccaagtat tgcctcaaaa atcacaaaga ggatatttag
    55501 tattatccca aacataaagc gagtattcaa atcactcaac tcttctggtt gatggtggtg
    55561 atggtgatcg tgcatagatt ttatttttcc taattaatta tttggcgatt tcaaaagaac
    55621 aagtcaatag tatttctgta aaagaataca ccacaaataa tcttgagaaa tgattcaaaa
    55681 aatttgatta ttccacaaat tcgtttagtt tctatccagt attctcagtt agtgtttcac
    55741 cttgactcta ttgctgtatt cctaagtatg tgaatacagg caaaatcaaa atccaaaatg
    55801 aaatcttttt taaagaaatt tatatttttt ctgtttgtgg tgattacaat cacattgata
    55861 cgaacttttg tttttcaaat ctatatagta aatggaaatt caatgcttcc aacactcaag
    55921 acgggagctg ttttgattac aaataaatat ttatttgcac aaaggattcc aatcacaaac
    55981 caggaattta attatggatc accaattatc aatcgtttgg atatggtttt atttgaaggg
    56041 gaagcaggcg atatattagt aaaaagagca atcggtttac ctggtgattt ttataatttt
    56101 gccgatggca ggatattaat agactcggtt cctcttgatg agaaatacaa agtcccagac
    56161 agtaaaacac tacttctccc gcaaccaatt gtccctgaca gtgttttttt tccaatgcag
    56221 aaagaaggta gaattcctcc tgattattat ctactcttgg gtgataatag agaacattcc
    56281 ttcgattcaa gaaatattgg tcttattcct ttaagccgca ttagaggaaa ggtcttctac
    56341 atcttaaaaa attaaccgat aaataaagcg atgaaagagt tcaaagatag attattattt
    56401 ttattatcct gtattttatt tttttttgga attctaatca tccgagtcat ctacctcact
    56461 tttctaaatg gaaatatggt tgaggtaaaa acggatcaaa gagtgcaacg aggcattatc
    56521 tatgatagac gcggaatgga attagctctt tctcaagatt cttctacaat cggaatcaac
    56581 cctgcaaaaa tctacagcgc agaaatcaca gcaaacaaac tttctaaatt tctaaatatg
    56641 cctgcaaaga aaattgaatc tatgattctg gaaaaatcaa attattttct acttaaaaga
    56701 gaaatcgaaa attcaactgc aagtaaaatt ttagaaatgg ctctaccagg tgtgagagta
    56761 gagaaagaat ttaaacgagt ttatccaaat ggaacgcttg catctaactt aattggattt
    56821 acaggtttag atgacaatca gtcgttatcc ggcatagagt tgctctatca caaagaatta
    56881 cagagttatg tagatggaga aagtctcaga ggaaacgata tttatctcac gattgatagt
    56941 ttgattcaat acagactcga aagcgctcta ggaaaagcgt ttgctcaaac gcattccaag
    57001 aaagggattg gagtttttat ggatgtaaac acaggaaaag ttttagcaat ggcaagcttt
    57061 cccaattttg atccaaacca ttattctgat tttccagtag aaaatacaac caactgggcg
    57121 attcgatatg aatacgagcc aggatctaca atgaaaatct ttatggcaac catcatgtta
    57181 aatgaaaatt taatcgacct aaatgaaaaa ttcttttgcc ctggatatgt tgaatttgga
    57241 agtagaagag tcaattgcgg tgatagacat gaaatggtag atttggatga aattttgcaa
    57301 tattcctgca atgttggaat catcaaagca attaaaaaag ttccagacga gaaaatatat
    57361 aagtatttaa aaaaacttaa atttggaatg aagacaggta tcgcaagtga tgaattaaaa
    57421 ggaagacttc ccgctttaaa agattggact cctagcactt cttactatat ggcaattggt
    57481 caaggttttg cggttactcc aatccaactc gtatcagctg ccgcggctat cgtcaatggc
    57541 ggaaaggttc taactccaac tgttgtatcg catatcgcaa actcgtatgg agaagtaatc
    57601 aagcaatttc aatacgaacc ggaatatatt ggaattagtc aaagcacaac ccaacatctt
    57661 atgagagcca tgaccaaagt ggtaaaacaa ggaactggag taaaagctta tacagaagcc
    57721 gggggagtca ttggaaaaac tggaacaagt caagtttata taaaaggaaa aggatatgaa
    57781 gaaggaatgg tagcagcttc ctttcttgga tttttcccag caaacaatcc aaaaattgta
    57841 gggcttatta ttttatcgga acctgaagga gtagttcatt caggaggagg tattgctgcc
    57901 cctgtattta aagaagttgt agaaaatatt attccaatta tagatttaaa cgaaacggcc
    57961 gtttcatata aattagaaga agcaaacaca cctaaaacta aaatagatat tactcgggtt
    58021 cctgacttca aaggaaaaac attaaaagag tccttaacaa tattaaaaaa ttatgacata
    58081 aaatataaaa cttatgggag cggtttcaat aagaaacaat ctcctgccag tggcgagcct
    58141 gtcagaaatg gggatgtttg gcatctgtat tttgaaggtg attaattcag atcatgcgtt
    58201 atttgctttg cttactcgtt tgttttattt tttctcagtg tagcacaacg cttctacaaa
    58261 gagaaaatta tatacagact agaaatgcgt tagaagataa agatttagaa aaagcgatta
    58321 aagaatttcc aaacggtgaa agcaatagct ttattacttc tatggaaaag gcttatttaa
    58381 atctaattca aggtaagcct gaaataaatg atttaatccg atatgcagag aaaatagaaa
    58441 agagagtgcg atacagtgcg tctagggaaa taaaaacctt tttctatgta gaaactcctg
    58501 agggatacta tgcatccgaa catgaaatca tatggcttca tatgttactc agttgggggt
    58561 attctctaag aggggattat gaaaaagcgt atgtagaagc aaaagttagt tccgacttac
    58621 ttagcaacgc ttggagtcca gaaggcagat ttgatgatcc attgatgaga attattttag
    58681 caagtctatg gacgttatgc ggacattggg aagaggctca agttgatttt agagtagcag
    58741 ccacactaga tccaaattta caatgggctc aacaactagc cgatttgcca gaagcaccaa
    58801 aagattttat attagtccta ggaggaactg gtcctgaacc agtatggaat ccgcaattag
    58861 aattaaatcc actcaggggc tttcgatctt tagacttcaa gtctaattct attaaaagca
    58921 aattgacact gagagactcg aatggaaaga cgatagacac acatattacc cctgattcaa
    58981 ccaattggta tactcgtcat gagataagag ataatgaaat acaggattta atcaaagatt
    59041 caatctatgg acaaaatatt gcaattacag cagtcaaaga aggcgggcga tctgtcttag
    59101 gcgtaaccgc tggagtcttg attgcgacag gcggaattgt tctaggggga gggattattt
    59161 atgcatccgt aaagtatggt tctggtgata gcactgtcga tggagtagcc cttggactaa
    59221 ttattatggg tgcggggatt tcgaagggct attcaattgc agaagaagcc gtttcggaaa
    59281 gtatcacaaa tgcaaaaaaa gatttggata tatctagcaa atatagattc gttagatttc
    59341 ttcctgacta tgcttgggtt ggctatagta atcaaaaatt aaaaacgcca attagaattc
    59401 taaatcaaaa tcaagttgtt tccgagatta aggatgctag gaatgcaact atcgtttcaa
    59461 tagattacta tcctgacgtg ataaagaagt aagtgttttt atcttgaaat aactaacttt
    59521 aaaaagtaat cgtatgcaat tctaaaattc acgccttcgt gaatcagttc ttttcctttt
    59581 tttactgtat aatattggtc tattttggaa aaagtaatat tcccttcctt ttttacttcc
    59641 ccaccatcaa cactgctttg aatttgtttc aatataccaa atgttctcat tggtaaattg
    59701 cctttattcc ttgatccttt tttggtcatg taatcctcaa tctatttcat cggtatcaag
    59761 ttaagataga taatttattg tattcaaaac aatcaaggct aactccctat ctacatttat
    59821 attcagtata tcgtttgaaa acaaatgcgc aatatatcac tgtagttatt atttttttaa
    59881 atgatatatt ttataaatta tcaaatactc atacattaac aagttactaa gaataaaaaa
    59941 caagtataga ttttttcgtc aacggaaaat cgctgtttac cgattattat ttaaatctca
    60001 ccaataatct cagctttttt taattcataa tcttcttttg atattaatcc ctttttatac
    60061 agcttcaaca attctcttaa tctttgctct ctatttccca caggaactgg tatgggctta
    60121 tcagtatttg cctcaagaac tttatttaaa tcaattagaa tccaagaata attttctaga
    60181 gatgtaatat catttccata agatggttta ttttcgctac tacaagtagg atcttgcttg
    60241 aatttaaaag ctatttcata tttcccataa aaagaaagta aacgatcccg acggcaggta
    60301 attttaaaaa aagtaggatt tgcccaatcc gtaaatgaaa attgggttcc aaaattaata
    60361 ttgtcattta cctcaccaaa tactatttga atatgattgt catctctatt tagataaaat
    60421 gaggttctaa aaatacgtgc gtaaggggat gtcttatctt cctctttgac tattacaaaa
    60481 taagtcttat tattttttaa agaaagtatt tctaataacg gaggaaccat ttcatctatc
    60541 gtttcaacac tccacagact atctctatct aagaaacgaa taaaacttct ctctttgcgt
    60601 aaagtaagaa agattctttt aagctgtaag tcagataaat caatattctt tttttcttta
    60661 ttatatttct caaaaatgga ttgattcaat tcataaatag ctagattgct acctgaatat
    60721 tttaatttac tttttagccc tctctcaaat gagatgcaac taaatacaaa tacacaaatt
    60781 gaaaataaat atatccattt cataaaaaga tttttcataa attacgaatc tcattcttta
    60841 attcaggatt acaagaaaga aactgacttt tcaaaaaatg aatctcttca attatagcaa
    60901 acatgcctac ttttttacga atttttctaa cttttacaat tctatttcaa atccctctgc
    60961 ttgcaggaat gaaagaagca aaaaaagcat atgcacaaaa gcaatttcaa aaggctatca
    61021 aactttttac cgagtataca aaagaaaatc catctgatgg tgaaccgtat atgtatatgg
    61081 gatatatata tgaatcgcaa aaagattttc caagatctat gatgatgttt agaaaagcag
    61141 tagaattaaa tttaaatcca aagcaccgca agacaagtta tctaaaaatt attttattct
    61201 atcattatca tcaaggctgg gatattgtcg ctcactatgc taataaattt ttacgattag
    61261 atccagagaa caaagaagta gcaaggatgc gagatagagc atatgcgaat aaggggcatg
    61321 accccggtgt tatctcattt gctagaccag aagacatcgc aaaagcaaag caaaaaaaaa
    61381 actcggaaga gacagagaag ttagcggcaa acaaagagaa accgcaggaa aaagaggaac
    61441 catctcaacc caaatcaagc gagacaccaa gccgcaaaac atcagaagag aaaaagtggg
    61501 aattgtcgct aaaatacttc caacaagagg actaccagaa agccgacaaa atcatgcaag
    61561 agctagtctt gcaaaatcca aacaataaaa actatctata caaagcaggc attgctaaac
    61621 ttagattagg agaatacgaa aaagcgctaa aatactttga atcatccaag aaattaacgg
    61681 atgaagatga caagatgctg cgatattaca ttacattaaa tgaaggacaa gccaaccata
    61741 aactaggaaa tacaaatact gctatagcac tatataaaaa agcatattcc ataaatcctt
    61801 ccaatctaat cttgccagtc cttacacgac tctattatga aaattcatat ttcgaagaag
    61861 ttctaaaaac ttcggatcaa attccagaaa cagatcaaaa taaattagag gctaatatgt
    61921 ataagtctct tgctttgata caaacaggta aaaagagatc aggaatgaaa tctctacttg
    61981 atttcgcaaa aaaaattaaa agaatttatc cagatataaa tacaattcct gaaaaattcc
    62041 atgagggtct tcttcaaatc ggattctttt actcgaatag aattaagtat aaactatctc
    62101 ttaaatattt aacacttgtg tcttctagca aaagcaaatc ttcaaagttt aattttgctc
    62161 tcggaaaaac ctatttctat acaaagaagt ttgacctagc gctagttttt ctagaaaaag
    62221 ttccagaaat atcagcggct aattatctgt tagccaaata ctatgcgaaa gaaaataatc
    62281 taatcaaaac aaaagaatat ctttctaaag caactgcaaa aaaagaaatc tattggatca
    62341 aagtaaaaat tgatccttat tttaaagact taaattcaaa tcccgagttt atgacttttg
    62401 ttgagacaaa gggaggaaag atttcacctg caaagcaaga tcttccgatt acaaatgaaa
    62461 agaagataga gacgaatatt cccaaaattg agcctcctgc cacaaagcca gaaacagttc
    62521 caacaaaaga tatgaatcca tctactgaaa ctgcgccaac tcctaatttg ccgcagaata
    62581 ctgagaatta aaatgggcag tattgtttca tcgcatcgac aatctcactg tccctaggat
    62641 tatttctttt aatcaaacca tcgacggttt tattccactc ctcttctttt cgattctgac
    62701 caaatttgaa tttcgcagag atagaggaag gagttagcct taatacattt actcctttta
    62761 tttgtttttt atattctaga tcttcaagat taaaaggaga atat
//
LOCUS       DAOQOS010000014        59697 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_188_length_59697_cov_6.090255, whole genome shotgun sequence.
ACCESSION   DAOQOS010000014 DAOQOS010000000
VERSION     DAOQOS010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 59697)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 59697)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59697
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_188_length_59697_cov_6.090255"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(123..1094)
                     /locus_tag="PLF66_04750"
     CDS             complement(123..1094)
                     /locus_tag="PLF66_04750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63381.1"
                     /translation="MEFLEILSERLYKENDLSDFVWALFKSNTQFKNYFLNFIKCPDS
                     SNGFEIEREYIFPNDNKRIDFYLYKGNKQKYFIENKIFDIGNYHFEEYSNLIDDPNSI
                     KILISAHTIDNYNYKIGEKYGWTILYWKDFISSLEKEKLDPIILDFINYLKKVCMIKE
                     IELIRFQANALKSIFYFNNLLDVIINCSNQEIYKCESYYKQSRAYENHYLGKYYTLKK
                     HDKIFSYSAIGIIFNTENPICIWLDHDWNKTINDFLMKDKNNILDENYKIDNYAITFY
                     MSQKEYESFVNSDKDNQFKVLQSFFVRVNNIIEKSYEGATSHRTTGA"
     gene            complement(1947..2438)
                     /locus_tag="PLF66_04755"
     CDS             complement(1947..2438)
                     /locus_tag="PLF66_04755"
                     /inference="COORDINATES: protein motif:HMM:NF023276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3368 domain-containing protein"
                     /protein_id="HMZ63382.1"
                     /translation="MKIISNTSPLVALAKINRLNLLPLVFTEITIPKIVEKELLAKAG
                     EEGEFLESAFQSFIKVVKNPVIDNELLEKISHLDKGEQSAIALAYFEKKNLLIDEKLG
                     RNVALNMGINVIGFFTVLLRAKHLKIISSVRSDLDSLRNKNYWIANSLYEKMLDLAQE
                     SSS"
     gene            complement(2435..2686)
                     /locus_tag="PLF66_04760"
     CDS             complement(2435..2686)
                     /locus_tag="PLF66_04760"
                     /inference="COORDINATES: protein motif:HMM:NF015630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0175 family protein"
                     /protein_id="HMZ63383.1"
                     /translation="MLLERNELNVLNSVQMTEKEFVSQILLMAAIKMFELNKLSSGKA
                     AELAGMTRVQFLDVCGSYKVPVIDFTQQELENEIELFKT"
     gene            complement(3496..5250)
                     /locus_tag="PLF66_04765"
     CDS             complement(3496..5250)
                     /locus_tag="PLF66_04765"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HMZ63384.1"
                     /translation="MKTNKIKTLLTISTSLNSNLDIFQLLPIIMLHAKDLLEAEASSL
                     FLFDPSDNHLYCEVALGEKGEVLQKYIRIELGEGIVGWVAKAGHPLLIEDAYKDPRFD
                     SSWDQKTGFKTSSIICVPMYQKHKLIGILEVMNKNNNEAFNSDDLELLEYLADLAAIA
                     IENSALNENLRKRIMELSLLYDFDKEISTNVNIHEIGDWLLEHCLSGLEAKSGSILLW
                     NQKEGCLKMFKSKGLPPDVVKNIKIFPGDGIAGWVAENKEPLLIQNLDTDPRFKNSKR
                     ANYENNSLISVPLLIQNELIGVLNINNKKDGYSFNRNDLKLTCAISERLALAIRNAKY
                     FDEMNLSAEENIRARKLIERIIPPDVPIVHNLTVRSKYIPFREVGGDFYNFFPLDQNR
                     IGVLVGDVSGHGLSSALLTVMVHTVIQSFEKNLFLSPANFLIYLNQCLADKMGGYFLT
                     AFYCVIDLEKESLFYAKGGHPSPILFRKESGNCLQLKSAGKLIGILPNALYEERSIPF
                     LKGDYLLIMTDGLLETMNEDTGKAYNMERLEDFLNENPNLEFEKLHDSIFQDVFQYVE
                     TKQFSDDVTVISIYRNEN"
     gene            complement(5345..7063)
                     /locus_tag="PLF66_04770"
     CDS             complement(5345..7063)
                     /locus_tag="PLF66_04770"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HMZ63385.1"
                     /translation="MFFRFCFLIFISIVSANAENPLSPLKLDSPRETMKMFLSSMNEY
                     KKGVEKNDESKKLAIEKSIKCLDLSEIAPLVREEKGKETAIFLKEAIDRVYVLELEKI
                     PETAVDKWSIPDTEITITKITSGNRSGEYVFTAETVASASEYYRKTSHMPYLAKTGGG
                     AAYSLPWLETIFPKWAREKWGFFFIWQWLGLLVATIISFLLKYVSKFFFRLAIKLTRN
                     TSTRWDDFILTTLARPVAYFFSIAYWFSFLYLSGIEGKIFTTFNFILKVCLGLNFIYF
                     LYKLSDLAVDLLKDNSVRTKNPIDDKLIPLMSKTLRILFVTVGFLIALQNIGVNVVGL
                     IAGLGIGGLALALAAKDTAANLFGSVMIFMDKPFKIDDHIIINGKEGIVEDIGFRSTL
                     IRTWEDSLVTIPNSVVANADIENMGVRRWRRNVINIDVTYDTNPEKMEAFLEGIKEII
                     MKNPLIVADTVHIGFRDFGASSLVIYLQFCLAVDSLPKNLEGRQLILLDVMRLAKEVG
                     ITFAYPTTSLYVESLPDKKQKQEKEKTITTFKNIIANFGKDGKLSKPKGAGIFVSPYH
                     DLPETK"
     gene            complement(7105..7611)
                     /locus_tag="PLF66_04775"
     CDS             complement(7105..7611)
                     /locus_tag="PLF66_04775"
                     /inference="COORDINATES: protein motif:HMM:NF025160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HXXEE domain-containing protein"
                     /protein_id="HMZ63386.1"
                     /translation="MLEFEKNSFYKKLIWLFPIAFFFHIIEESNGFPYWVTYVLEGKM
                     EISLFYINNAMFMVVLIGLTLFAAKKQNRLSTFLLFLWVSGQQFWNFVFHIYTQFKFN
                     AYSPGYFTAIFLYFPIYLYLTYLALRDEHIEWKHWLFCFFAGSFGMAFTIWSGLYHFG
                     AVPWSKWI"
     gene            complement(7871..8878)
                     /locus_tag="PLF66_04780"
     CDS             complement(7871..8878)
                     /locus_tag="PLF66_04780"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002179487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HMZ63387.1"
                     /translation="MLPVKQIGVIGAGSYGNALALHVARKGFPTKIWAYEKDVVEEIN
                     QNHTNSVFLKGYTFPENLTATNDLEECMNFGEAILSVMPTPHVARMMKQATPFIRDGQ
                     TIVSCSKGIENESLEIPSEIMERVLPEKFHHQLAYLSGPSFAKEVASGLPTAVTIASA
                     NLKLAAHVQVFMSDKRFRCYTTSDIIGVELCGALKNVIAIAAGIADGLGFGYNTRAAL
                     ITRGLGEINRIATQKKANPLTMLGLAGMGDLVLTCTGDLSRNRTVGFKIGQGEKLKDI
                     LGDMRMVAEGVLTSKSAYLLSKKLGVEVPIMEQVYRILYEDQDPKQTVENLMSRELKG
                     E"
     gene            8996..9226
                     /locus_tag="PLF66_04785"
     CDS             8996..9226
                     /locus_tag="PLF66_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010514840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HMZ63388.1"
                     /translation="MEKEKLLKRITINPEQCGGRPCIRNMRIRVSDVIDMLSEGMLPT
                     EIIDDFPYLELEDIKASLLYVSRYINHTVIAA"
     gene            9223..9564
                     /locus_tag="PLF66_04790"
     CDS             9223..9564
                     /locus_tag="PLF66_04790"
                     /inference="COORDINATES: protein motif:HMM:NF037832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HMZ63389.1"
                     /translation="MILWIDAQISPKIAKWVEDTFSIKTYHISEFKLTQTTDREIFLK
                     AKQNSTDETICILTKDIDFVLLLEQYKVPPKIIWLTSGNASNEKIKLTLSKALKEALH
                     LLNQEDLIEIR"
     gene            complement(9666..9947)
                     /locus_tag="PLF66_04795"
     CDS             complement(9666..9947)
                     /locus_tag="PLF66_04795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63390.1"
                     /translation="MAFGIKGIVTKNKTYTVLLIKDKFFPEGEECILGNNKEYVTLDC
                     FKGEIKFDGVDGNYEYKFNADGTCINTYNTKEEYGGRTSRNGKIFRHKD"
     gene            complement(10057..10230)
                     /locus_tag="PLF66_04800"
     CDS             complement(10057..10230)
                     /locus_tag="PLF66_04800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63391.1"
                     /translation="MAFEAYLNLFWTNWKGKKPDKSLQVLIRASLRETYYSLGFKKVK
                     ALESIYQMTKETF"
     gene            10424..11602
                     /locus_tag="PLF66_04805"
     CDS             10424..11602
                     /locus_tag="PLF66_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HMZ63392.1"
                     /translation="MTDTTGILLNFTEEQMQLRELVRDVVKKEVIPNRMYYDEHNEYP
                     RKVLDKFKEAGLYPAMFEEEAGGLGLGILAQMILVEEVSYGCLGINVSFATTKLGALP
                     IQIGGTKEQKEKWLAPLVTGDKIAAFGLTEPGAGSDVPNMGTTAVKKGAKYVLNGTKQ
                     WISGAGQADIYTVFAITNKERGTRGISCFIVEKGTPGFSFGKKEDKLGIRCSETRQLI
                     FEDCEIPEENLIGLKENTGFLTALKTLNLSRPAVAISAVGLSQGAFDCAIQYSREREQ
                     FGVKIGSFQAIQHLLADMAMRIEASRLMTYKAALLCEMEHKDMAKFSAMSKCYAADTA
                     MFVATEAVQIYGGYGYTKEYPVEKYFRDAKILQIYEGTREIQKNEIAAGLIKESATRK
                     "
     gene            11605..12348
                     /locus_tag="PLF66_04810"
     CDS             11605..12348
                     /locus_tag="PLF66_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004419847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HMZ63393.1"
                     /translation="MKNLFDVSGKTILITGASRGIGKALAEGFRDAGAIVYGTGTKPE
                     TIAWMKEANIEGRVNNLLAEDKIKDIIGEIKAKHGKLDVLINNAGISLDKPGSHLNEK
                     EMSDLIDINFKAVFRASQAYYKSHKLLGGNIINIASVLGLRGFTLATVYSGTKGAVIQ
                     LTLACAAEWIRSGFRLNAICPGFIDTDMVGNMKSKEALMEGIKSRIPMGRMGKPEELL
                     GTAIFLASDASSYINAQTIVVDGGMTNIV"
     gene            12410..12577
                     /locus_tag="PLF66_04815"
     CDS             12410..12577
                     /locus_tag="PLF66_04815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63394.1"
                     /translation="MNTDIIRVIEKLRYTNISSSDSRELQQRKNFILNLRDYTRIAMI
                     HIRNFPLGISK"
     gene            12588..13148
                     /locus_tag="PLF66_04820"
     CDS             12588..13148
                     /locus_tag="PLF66_04820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63395.1"
                     /translation="MKILGFIAISFYFFYAGYSYSKGSLSNALWICHIGCFLVGLGLL
                     ASINILSSIGFLLLSVGNVGWALYLLAGGELILPSILTHVGGFLIGLFVVYKNGIEAY
                     TYLYSLIAFILLQILSRLSTDAKENVNLAFQIQEGWEKIFPSYPIYLLVIYLLLFFGF
                     FGIEFLIQKLKSQTRIEETKFNSEPQ"
     gene            13145..14206
                     /locus_tag="PLF66_04825"
     CDS             13145..14206
                     /locus_tag="PLF66_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010575787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HMZ63396.1"
                     /translation="MKKFKIFLFFLILLVSYFTFKSTPIDPVAFTPPEPREMNGPLSK
                     NEILQKAELLGLRKIHGPEGLAIDRNGIIYGGTAEGNITRIYPDGKVEPFINTGGRPL
                     GIEFDANGNLIVCDAWKGLLSVSKEGKVTTLSDSADNVPFRFTDDLDIASDGMIYFTD
                     ASSKFHQPEYLYDLLEGKPHGRFLKYNPYTKETTVLLKNLYFANGVALSKNEDFVLIN
                     ETYRYRITRFYLKGEKKGTSDIFLENTPGFPDNITSNRKGSFYLALFTVRNPMMDRIH
                     PHPFFTKLMSKLPKFFWPKPKPYGLVIRLDESGNILESYQDPSGKHLKEITSALEYNG
                     FLYLGSLHNDRIGKLELSK"
     gene            14292..15299
                     /locus_tag="PLF66_04830"
     CDS             14292..15299
                     /locus_tag="PLF66_04830"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002981960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HMZ63397.1"
                     /translation="MRASIIRKYGNNDVIEYVEDFPKPEIATDEVLIEMHAASVNPID
                     WHVMKGDVKLLFNYKMPHILGNDGAGTVVQIGSNVSKFKIGDEVYFRPGKKKITGTFA
                     EFCSVKEDEVALKPKNLSFEEAAGIPLVGLTAWQALFDLGNLQKGQKVLIHAGSGGVG
                     SIAIQLAKTIGAEVATTTSTSNLELVKSFGADTIIDYKKENFKDKLKDYDLVFDTLGG
                     KTREDSYPVLKPNGILVSIAGIPTPETADHYGLNFVIKTLFYLLNFKNSSIAKKHNVI
                     YKYFLMNASGEQLSKITKLIEEKKVKPLVDKVFSLREVKQAFEYQQTGRVKGKVIIKI
                     K"
     gene            15404..16579
                     /locus_tag="PLF66_04835"
     CDS             15404..16579
                     /locus_tag="PLF66_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002743660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HMZ63398.1"
                     /translation="MNFDIPKEVEELRLKTREFVETVVMPHEDDYDYNIGRLPENVAE
                     DLRKKVKSAGLWTPHLPKSEGGLGLDPVGTAIIFSELGRSPIAPFVFNCDAPDEGNMH
                     LLHLAANEEQKEKFYYPLIKGEIRSGFAMTEPAPGAGADPQSLMTNAEKVGDKYILNG
                     RKWFCTGANGAKFLIVMSKVSGSFRRSTLFLVPTDAPGYTMVREVATMGSHGQGGHCE
                     LNFENVEVPQSMILGRVGEGFKWTQERLGPARLTHCMRWIGLARRSMEMAREFAKKRE
                     VFGAKLADHEGIQWMFAESALEIESGFLLTLKAADTLRKGDDTRQIISMAKWHVSEIL
                     CRCIDRAIQICGSYGYSRDSKLELFYRDARAARLADGPSEVHKMVIARNLMSGKFEF"
     gene            16763..17167
                     /locus_tag="PLF66_04840"
     CDS             16763..17167
                     /locus_tag="PLF66_04840"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HMZ63399.1"
                     /translation="MNQIYRLILILFTIFPIVLASKEEKDKCDTYIEKGYQESKVLTK
                     KPIIKINFSLKDFFPKEAKEKNIKTGKSVVQIFLNKKGKLVCTSIRQKSEGYGFDQAA
                     LQILRKAKFRPGEVKGKPVDSYLTLPIEFSSE"
     gene            complement(17363..18196)
                     /locus_tag="PLF66_04845"
     CDS             complement(17363..18196)
                     /locus_tag="PLF66_04845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63400.1"
                     /translation="MTKTQKQISFGTIVMIVFLFAPPLFVNGWKFLVRDYTIYEIWSH
                     LLLDLSVLVLFIFLLAKNPLEMPIVYYSLFIVFCISYLNMNTPTSTLDWFANALVLIA
                     LGIFVFPRLSGLFPYVLVGLFISYYGVIRFMLPEMNYTEAKEVLQKKNPSETAKEFIH
                     ILANYERNMLGREKYRYLLLKDGDFTNRLKKANTLHSDLFPSLQKQGISFLMWQEANI
                     KIENDSITLFGRLPGVANDDSLKGVFAKVEPVQIKITRLEDDTYRVVDFPEKILVQKT
                     E"
     gene            18466..19173
                     /locus_tag="PLF66_04850"
     CDS             18466..19173
                     /locus_tag="PLF66_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016752478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HMZ63401.1"
                     /translation="MRNRVNIHDNGLKVLVVDDEEDISDLIRFNLEEEGFQVSVCQNG
                     LEVLPKVDKDLPDLIILDIMLPGIGGIDLCKRIKEKFTVPIIMVTAKTGETDAVLGLE
                     LGADDYVRKPFSPRELMARVRSVLRRYEDKTEEDKAGNITIGKIHLNKKAHKAFVDGK
                     EIDLTLIEYKILLLFMSNPGVALTRDKLLDKIWGHDIYVTDRAVDVNIKRLRDKLLSE
                     KERLETVRGVGYRFSDA"
     gene            19163..20548
                     /locus_tag="PLF66_04855"
     CDS             19163..20548
                     /locus_tag="PLF66_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017863076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HMZ63402.1"
                     /translation="MMRSLFSRVLLSNWLFLVILLGTALILLYLETLVAPEFKLILLV
                     LYIIYSMLIAFHSSYKIANSVTEQLSVIQKKTMDINAGDFGTLLAASDIRELGDLVSS
                     INSMSNRLQMQFTDLNLEKEKFNSLLQNLREGVFAISPDKKILFQNQSIPKSLIPTNS
                     QFRSIQDVIQNKRLLEFLIEHIDSTQDGKIGIEDNKHYYKVWFYSLKSNNQILMYIGV
                     ISDKTDERETQMLREQFVQNASHELKTPITSIKGYAETLQSKLHVSQDSVEKRFLDAI
                     LRNTDRMIRIVEDMLTISKLESQNYFFQPEKVNLSDIVQNLKFTVEGFIRLKNQTFTA
                     EVPENIYLYADMVLIEHLLLNLIQNASTYSGDNQPIKLIANTNDAFVVIKVIDKGIGI
                     KEDHIERIFERFYRVDEDRSRKGGGTGLGLSIVKHIAKLHSGWVSVASKPGEGTTFTV
                     NLPIDRKKPMS"
     gene            20569..21720
                     /gene="argJ"
                     /locus_tag="PLF66_04860"
     CDS             20569..21720
                     /gene="argJ"
                     /locus_tag="PLF66_04860"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015681773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HMZ63403.1"
                     /translation="MYPLGFYSFGKNIGIKDSTLDFSVIYSEKICDATAVFTQNNFPG
                     SPVIVGREHIQDGKLQAIVINSKNSNVATGQKGIEDARNTCKTLAANLGIRSEDVLPS
                     STGVIGVPLPIEKILTACQTAKENLKPGNLEQVAEAIMTTDKKRKISFKVIESNGVKG
                     VIYGMAKGAGMIEPNMATMLSYILTDLKPTSSNLYSFLKSSVDKSFNCMTIDSDTSTS
                     DTVVLMSNGLAGEIPDSLFQQALDEICIDLTKQIAMDGEGATKLIELDILEAKDDQQA
                     RKIGKSILNSPLIKTAIYGGDPNWGRFVMAIGKVFDEPISFEKLSILIGGISVKGANS
                     ETLKKLSNYLKNNSIIEISVYLGAGSSSKRFWGCDLTEGYVQENAYYTT"
     gene            21720..22967
                     /locus_tag="PLF66_04865"
     CDS             21720..22967
                     /locus_tag="PLF66_04865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63404.1"
                     /translation="MKEKFSFLYSSDYRFKGYVITNLVRLISIILTISIYFSLHQLIP
                     KSLSYSLQIMMIGTIAISFIIIFPIQDYLEKIIRQKFVSQYLDEDLSAGRVVNVELNE
                     LISNLFPDMVKISESKEGRMVLLIREPADYEMYVYLNGMRRKVKSKSFQIKDRLLEFL
                     SDKPEGITAANLLGHNEINEDFISLKVSYVIPFIYRQRIFGFLGVSNIPSEKSISNLY
                     ILASKAAIAVYNHILSSQIVLHKKYKREFEIANRIEGLIFSAKVPIIKSFKFEAYQKD
                     PNVLVEFFRNDDGEWLFILLALNKARIGTGLVSSHLLGKMYSQSLLRRKHSLNSIKSI
                     TEDMLRKLSWEDGYEMVVGSLREDLSKLTFSQKGVNFRITNDVDTSKSLISVGWRYTV
                     DLKENPVIFIYYKSSKIISVQNI"
     gene            22971..25286
                     /locus_tag="PLF66_04870"
     CDS             22971..25286
                     /locus_tag="PLF66_04870"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HMZ63405.1"
                     /translation="MKNSLIITIASICFITFNLFILRGIGLGSNYPKRFLYFYPNGYI
                     ANTDFDNTHMLGINIEEERIPKIRLEKNKESDFTSEFFLQYKTLTDFSPHIILSLILL
                     ITSFWFLIKYSDVYISLLFFDLSIFVYSNFTVLAFDSYYFLFYLSFVFMVFLTIHLGF
                     RLKGKEVSIRWIIPEICASAIVAFTAISQKENSQFFNEFVKLCGNLVCIGAAGCLTVL
                     IYDTIKYKITGNPLIKKIAVIISILQMVIIPYSVFEFNLFRVFNNLHYSLYITFLSFP
                     LLFVYGTYRYTMIREQVYFVSSVVIFTLLSSLIVFYILLMGAVNYLLETFAIQNLEAI
                     NVLFLIISTYSLVLAKNKIRDFTEYWHFKRNEKLTQTLEQMIDLVRSQQPMRITTTKL
                     VKAITETLDIQKVVILIPSDLIPDEIKNENILRIHSKSEVWSFFSKEKDITVTTSVMY
                     GSGIRNNVYTFLRQLNIQLAFPMLGRDEKESVVGLILIGQKNIPGNFTLGELTFIKEC
                     AKLAYLLLNNYKLLLADIEKKKMERKLKEAMILEETIHPPMSSKINNRQMEIKFFSDP
                     AVSISGDYVDFIPINEKQVFIMLGDVSGHGLGSGYLVSAIKAIVHDQIESGVDLGKLF
                     KNINNFLIERYSGTEFMTLIGGLYDSGSGRFEFINAGHLSPLILRATGEIEAKKSGHR
                     ILGVTVGPFTTDSIQLNVNDKLIIYSDGVTETFNPLDEIYGEERLRRFLAANKDMPSE
                     NLLEFLKRELENFRKDLEISDDTSFIILKRM"
     gene            25288..25845
                     /locus_tag="PLF66_04875"
     CDS             25288..25845
                     /locus_tag="PLF66_04875"
                     /inference="COORDINATES: protein motif:HMM:NF013653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HMZ63406.1"
                     /translation="MNTIKDKIIKVEQDIDSKKIVSVQSVAFKSINANELKEKKHLID
                     CDLDSILVSGNYKLIHTKKINSDSDISFSGLLPVVTLDTMGKVLMQAFVNEEALYQSI
                     SSGYATYFSRSRNKVWLKGETSGNKQEMINIEFADSFFIYIVRQELASCHEGFYSCYY
                     RQLLADGSLAQIDFDRKFLPEKVYG"
     gene            25878..26882
                     /gene="mltG"
                     /locus_tag="PLF66_04880"
     CDS             25878..26882
                     /gene="mltG"
                     /locus_tag="PLF66_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004421649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HMZ63407.1"
                     /translation="MLIKRILPAILGILFLGIGGMFLIDSIKGGAPGDGSSKYELIVS
                     AGESSTKITKDLASHGMIQSSSYFAYLLKISGNTNKIKQGVYSIDNGMTARKIMDILV
                     DGKVKMISFTIPEGYNNRQIGDVLTQKNIAKSRDEFLALASNEEILKKYKIPAKSTEG
                     YLFPETYTVPYNYPLDKTVEMMLKRFFLNLQKIDKAKDVSPEDLHKKVILASIVEREA
                     KKKEERPLMAGVFIKRLKKGIALESCATVQYLFEKPKKRLFEKDLLTPSDYNTYLHKG
                     YPPGPISNPGMPALLAAFNPEETDNLFFLLKPDGYHYFSKDHKEHAQAKKKYIDVLYE
                     "
     gene            26875..27135
                     /locus_tag="PLF66_04885"
     CDS             26875..27135
                     /locus_tag="PLF66_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002976132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63408.1"
                     /translation="MSSINFDNLDKEIFGINKELQDVQKALEEVKKRQDSNKSAKKEA
                     LVFIEKAEAVIEKAEKGKIKLSEDQVERIKETLKKILLIFKS"
     gene            complement(27132..27956)
                     /locus_tag="PLF66_04890"
     CDS             complement(27132..27956)
                     /locus_tag="PLF66_04890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HMZ63409.1"
                     /translation="MKNEITLRTNIFDLDWNRHVTSRTYERFSQEGRSQILDSLGFPI
                     RKCMEEGILLIPEFTQVRFLSQQFSDANLRIETQLVAYRENQLEWNHKIYGEDNSLAC
                     ELRLISRLEKASGETFVLTSSSDRFEIPTIHAFKGTCKQLIHDYTFLYSDLNCFWRYS
                     PDVVWKAFEEGRWLFFSKVADTSKIAKIDTTSFFMGGKLRFIRMPNGGEKVKIVTWIE
                     SVEKIRFYLRQDIISETGEIIASMRDEQLFVSLSTSRPKKAPLEFVENIKDFIENF"
     gene            28128..29402
                     /locus_tag="PLF66_04895"
     CDS             28128..29402
                     /locus_tag="PLF66_04895"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HMZ63410.1"
                     /translation="MDTTLGININTGKSYTVLIADDSFSDRQLIKRFLQADQFEVLSD
                     VPSGEGVIDYLKYSKTVPDIIFLDYKMEPKNALETVKEIRPTYPDLMIVIVTSNVNKE
                     AIAELARYKITAYILKPISKSIIDQKLIQILGRKDLVVESKLVKKHALKLEDISIPPL
                     PGIAQKVLLFEGDPVSGSSELEQIILPDKSICADILRIANSAFYGRSKKVQTIKDAIT
                     LLGLKTIKNIVMLQSKRHMAKNLVYTDIFKKYLLELPILNALVALDLSVPMGLKSQRE
                     ELFLCAMMIKIGMTILALNSAKVYNDMLTQYDKVGGDLVKLEKEEFEISHVDVGVHIF
                     KIWQMPAVFQKTMKDQGFLTVEFNKVDDFDRVLRLSEIISKRLTHIPVPDSDIELAKH
                     ILRHYNSPEETLDVFDDEYYENIKSHPFFSSM"
     gene            29582..30004
                     /locus_tag="PLF66_04900"
     CDS             29582..30004
                     /locus_tag="PLF66_04900"
                     /inference="COORDINATES: protein motif:HMM:NF018663.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HMZ63411.1"
                     /translation="MAKVSIYLNFQGNTKEAFDFYKSIFGTDFETIVYHKDTAENGRP
                     NLPENEWNKIMHIDLPILGGIHLMGSDMLESMGHSLKIGNNTTINLQGDTLAETKRLF
                     ESLSNGASEIAPFGETPWGHWGCLLDKFGIRWMFIFPL"
     gene            complement(29999..31390)
                     /gene="mgtE"
                     /locus_tag="PLF66_04905"
     CDS             complement(29999..31390)
                     /gene="mgtE"
                     /locus_tag="PLF66_04905"
                     /inference="COORDINATES: protein motif:HMM:TIGR00400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HMZ63412.1"
                     /translation="MKEQVAFKPERDEEMISQQEKSRHLSVELIHSYIVSKNYTALAE
                     YIEKAHPADIAEIISSAKIEDALYVFRLCDKDKQKLVFVELGEETQAEFVGYLKLEEI
                     AFILEDIESDDATSFIAEIEPEKAEEILNSLDSKDSAIIRNQMSFAENSAGRLMSSDY
                     AVIKDNETASKGISSVRRAAKETENIYLIYVTDQKGIVKGFVQLKDLLLSHHKTKINK
                     IMKPVQTIHYNMDQEEVARFFRKYDVVSAPVVDDEGKIIGRISVDDVLDVVEEEASED
                     ILRLGGLSEDEKLKTSIWDSVKRRIIWLGLNLVTAMLVSTVVSLFEETIQRIVVLASL
                     MPIVAGMGGNAGTQAITIVVRNLATGELTLYNWYIAVRKELIIGILNGFVLGSIIFTI
                     TFIVKRDLTLASVIGSAMLFNMIVAGLIGSVVPLILKFFKIDPAIASSIFVTACTDTF
                     GFFCFLGLASFFL"
     gene            complement(31466..32317)
                     /locus_tag="PLF66_04910"
     CDS             complement(31466..32317)
                     /locus_tag="PLF66_04910"
                     /inference="COORDINATES: protein motif:HMM:NF025446.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4261 domain-containing protein"
                     /protein_id="HMZ63413.1"
                     /translation="MIKLIILFIAIFVLIFSGLILIYRGSRGTYKTTLTGIILLEDDP
                     LFNSSIIQRELETKYKMKISKQTSNDGIVIFTAEKYRIVILGVDAEIPKEEMETVAKM
                     SHIWQDAEKQIAEHLSHLIITVSTNVEDLVEANMQFTQILASVLRNTKSIGVYLGNQC
                     LVSPKEYFLKIADTMTKDDLPLNNWIYFGLRENNGKRNGFTIGMKEFGFREIEFLNSD
                     KTFDEIYKIIYDLAHHVLLSRKPLKRGETFDLSATENLSLDIQAGEFQDTESMHVITN
                     PKISSLQ"
     gene            complement(32310..33581)
                     /locus_tag="PLF66_04915"
     CDS             complement(32310..33581)
                     /locus_tag="PLF66_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004456448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HMZ63414.1"
                     /translation="MSGNLFSSGYEPLASRLRPEQFEDLIGQSETIQILKKLKRPVSM
                     ILYGPPGSGKTTIAKILSKNWKLNYKHLSAISVGVKEVKELITESKKIGTILLFLDEI
                     HRFSTSQQDSLLEAVESGQIILVAATTENPAFRINRPLLSRCQIFKLKPFTELELLQI
                     LDKALQKEGGKLELDAESKKIILNASGGDARRLLGIIEALHTIEPEKPWDKESIESYL
                     SSQVFNYDRNKENHYDFISAFIKSVRGSDPDASLYYLACMLEGGEDPIFIMRRLIILA
                     TEDIGNASLQALPLAVSALTALEKIGMPEGRILLGQVTTYLASCPKSNASYTAIDSAL
                     AFVRENGVNVTIPNHLRNAPTFLHKKEGASDGYKYPHDVEDHFILENYFPKELSDFSP
                     QFYFPTNQGMDKNLKERLKSLWEKTGRKKYD"
     gene            complement(33578..33799)
                     /locus_tag="PLF66_04920"
     CDS             complement(33578..33799)
                     /locus_tag="PLF66_04920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63415.1"
                     /translation="MSDQPTLFPPDRDSVIVDHLERYGLPPNAKFQIIALLEGRLNES
                     SLVCCHSGCVLCSELIYNCLQDVKKELAS"
     gene            complement(33799..34098)
                     /locus_tag="PLF66_04925"
     CDS             complement(33799..34098)
                     /locus_tag="PLF66_04925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63416.1"
                     /translation="MEQFSETDNQNDSEWGIYKGKMNWDKAMEEANKLGMRLPTREEL
                     LDAYQKGKLKSWLDNGYLFWTSDEFSSFYAYGLYVSSGHSYYYNKTNEWHARFVK"
     gene            complement(34167..34889)
                     /locus_tag="PLF66_04930"
     CDS             complement(34167..34889)
                     /locus_tag="PLF66_04930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63417.1"
                     /translation="MILIGLLFFVIASLGPELMSVSKKPKAIQPNSKTQIVRNSPKAF
                     KSKKALFNFRYPEHRSSTEIEKKIRQERRMATSEWKPKLDQSEMEMPTLDEIPEENSI
                     SSFYNTVHTKTTNSIQETREKMETIPIEEESSMIELNGLLFLDYSRTIPFQSKDLKSI
                     QWSENMFQNFKRIGVARVQHKNNSFKFITGNLLTDFAYSDIAEVIFFDQAFSIIPKKR
                     FLPVPLIFSEDVNKLKALIVKN"
     gene            35016..36257
                     /locus_tag="PLF66_04935"
     CDS             35016..36257
                     /locus_tag="PLF66_04935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63418.1"
                     /translation="MILEEEPVSISQKIYRIFAGLFIAVLVTMLIFTLIPGDAEQNLL
                     RMIIGQDQMTAGKVGDETIPIDYFKAARLECYYMYKQRFPTAADDASMLENCAFNQIK
                     SLKIDKVLANAAGYHVSDLSIKEDISNEARKIHSQSGTSAGYSSDEVRSVDDIYRSIL
                     QSVPMHYRQDTAASIELYERFLYTDVKESEEEQKIRSELENVRLSLSYISFSDEELSK
                     VIGEIPQITDAEIQKEYDASVANKTVPMGEDGKPQPLEVRKALIFNKLQSEAKEKKLS
                     ELKGKILSAKGAENANLGALALIAGVKIADLNKVSLSSIGPQAKENKTELPKFLSGSA
                     FLKDITNQAFGKGKVGGPYTDKEKTFYVEFKDLSIESGNSVGKNVAMNESNSNRTRMF
                     LYEIKQSLGGIFPIQRNKSVE"
     gene            36258..36722
                     /locus_tag="PLF66_04940"
     CDS             36258..36722
                     /locus_tag="PLF66_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020774267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheX"
                     /protein_id="HMZ63419.1"
                     /translation="MEIRAEFVNPFIEAASLVFKDSLKVELIRGKLKIKESPAPSHEV
                     AIVIGVVGSYKGEVVYSMNLDAAYKMAKQLVPSLDDESIKDEYKDIIGEIGNMTTGNA
                     LRIFATSGQFIDITTPNVIETNDSSIKFVKTTTLSLNLYSRFGQLEINIAIL"
     gene            complement(36719..38164)
                     /locus_tag="PLF66_04945"
     CDS             complement(36719..38164)
                     /locus_tag="PLF66_04945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63420.1"
                     /translation="MKKNTYLFSNKKSFTATLIVFSTIVFSQDLKKEIQIPFPENITI
                     EDPMDRNIRKDKPAIPTKEEEKKQSIRIPFPKDPVDKRESEVEFKQIEVTETKPELNN
                     PITNSNFPLEKIIVVPETESAKNAPVIESNPKTLNLEKPAKSVSNATELPSSNVSKDT
                     KPNIEPVKPIEKENIKPTTDIGKALLESEKSKSVAKPKSTEVNNLPKSKLDLGEYGAK
                     DKSPKSTEKTASQAKEAKEIKEPSAKKAETTGNLQTTNLPNVNKTSDKKGKDKKKADE
                     SSAPFERSRYYLNREDKTSAVSELNIASSGEGEKSNLAKMDQIRLLAQERKKNEAKSI
                     IESISDQDYKFKGLYELAVGLENSAKGDRKLKEEAIPFHLTIITEAPESNSIVPKSLW
                     ALSHLLFTIGDHTPALDHLSDIILKHSKSEYIDDAIYLSGRIYEESTTIRNLNRAKKY
                     YQMFLENANKPFFKNSIYLPFVKKRLELIEN"
     gene            complement(38165..38944)
                     /locus_tag="PLF66_04950"
     CDS             complement(38165..38944)
                     /locus_tag="PLF66_04950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63421.1"
                     /translation="MDFKKIKPKDVLGFLDDLVAKIPESIASIFRKLMIGVLLIALGY
                     GIYWGYLVGVGSAKQEGQELAKDTKSLFQEDIEREYNRKRKNIRMPSDDTLIGEDLYK
                     TPKQYERYGRQTEPNSIYSADDSLVENDKSIRSLKRKGDSSPLAELNDVPATQEFPIP
                     NRNENSESNKGKKRKLYLKDNFDDPLSGSESRDMEGIFDRVNTDPNSEKKILNRKDSK
                     DKIDSTGNMELPTKKTGSLETEGNPQKKDTKGKLLPLDGNQ"
     gene            complement(39000..40238)
                     /gene="clpX"
                     /locus_tag="PLF66_04955"
     CDS             complement(39000..40238)
                     /gene="clpX"
                     /locus_tag="PLF66_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020770752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HMZ63422.1"
                     /translation="MGKEKLHCSFCGKEQDAVKRLVAGPGVYICDECISLCTEIIADE
                     PAHLERPTHIADVPKPQEIKKILDQYVIGQHHAKRALSVAVYNHYKRIHFNVGKTDVE
                     LEKSNILLIGPTGSGKTLLAQTLARILKVPFAIVDATALTEAGYVGEDVENIILKLIQ
                     NADNDIKKAELGIIYIDEIDKISRKNDSASITRDVSGEGVQQALLKIIEGTIANVPPQ
                     GGRKHPHQDYLSIDTRNILFICGGAFVDLDRIVKSRVGVKTIGFNSDVNANVNDMTKS
                     DALRQVIPEDLLKYGLIPEFVGRLPIIATLEEADINTLKQIFTEPKNSILKQFSKIFE
                     LENVNLKFTDEAIQAVAAQAIKRESGARGLRAIVEDIMMDLMFQIPSREDVVEVVITE
                     ESVLKGEAPLLILKPKEKIA"
     gene            complement(40270..40869)
                     /locus_tag="PLF66_04960"
     CDS             complement(40270..40869)
                     /locus_tag="PLF66_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HMZ63423.1"
                     /translation="MPIIPYITEQTSRGERSSDVFSRLLKDRVIFIGSGIDDEYANIL
                     TAQLLFLEADNPDRDIYLYINSPGGYISSGLAVYDTIQYIKPEVRTLCIGQASSMASL
                     LLAAGAKGKRSALPHARIMMHQPSGGATGQASDIEIQANEILRVKGIINSLYQKHTGK
                     DISVIEKDTERDFYMTAEEAKAYGIIDNVIIDRKTVKAG"
     gene            complement(40873..42207)
                     /gene="tig"
                     /locus_tag="PLF66_04965"
     CDS             complement(40873..42207)
                     /gene="tig"
                     /locus_tag="PLF66_04965"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HMZ63424.1"
                     /translation="MEYKVTINDDATAKLDLTYSPEDIEDAFKKAYQRAAQKVKINGF
                     RQGKAPLDMVIKMLGDSVSEDAVTILLTDSINGLYPSLSFKAFKAPKIEIEKFERHNT
                     LVAKATYETAPEVTLGNYKNIPVEVYQIKITDEDIQSQLKDIQLKLAKHQAKEPEELS
                     QDGDLLDLDIETKNEAGEIIQDTKNNQYYIGLNPDQKDLDEKLIGLKVGEKKSFDYTY
                     GEKANPELAGKTFHFTVTANSISKVIYPELDDNLAKEWDETPSLEELKNRFKDRMKTY
                     VEDRMHAHYSEQLMGEIVRNSSYKIPNSLIEEEMHALYHQFTHDYNLDHMPMEKYAEM
                     LNTDLDSVKSNFQNRALNQIKYYLAIHEISAKENITVAPEELDAVFSKLNINELEKKR
                     HESRERIYRNIYENLISQKVYDFLLSNSNKQGNSEISISRAGEIVNGSKKDN"
     gene            complement(42239..42310)
                     /locus_tag="PLF66_04970"
     tRNA            complement(42239..42310)
                     /locus_tag="PLF66_04970"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(42276..42278),aa:Gly,seq:gcc)
     gene            42663..42983
                     /locus_tag="PLF66_04975"
     CDS             42663..42983
                     /locus_tag="PLF66_04975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63425.1"
                     /translation="MKLFIILNKVRLAGFAIMLIGILGNSYNWYQVLHSGVFFEKACI
                     LFPLFAFLGLSIVIYPMSKEDRISRFGTDQIPISAYPFGQKVLVGLGLLIGILQWLWL
                     KGLV"
     gene            complement(43196..44062)
                     /locus_tag="PLF66_04980"
     CDS             complement(43196..44062)
                     /locus_tag="PLF66_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000142697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase"
                     /protein_id="HMZ63426.1"
                     /translation="MTYSNTQSSFNSSVDNTKIFYQSWTKPNANRLLVIQHGFGEHSD
                     RYKNILEKLKDSDFSIYALDSRGHGRSEGIRGHVDQFQYYIEDLSDLVHIALEKEKKE
                     KFFLLGHSLGGVIALQFTLEVHNQDRMHGLIVSSPGLKVKFDLEKLIKRELAKIFANF
                     LPSFVVDANIDVNLLSHDKSVVEDYKKDPLNHGKISFQMANHLFHLSYAIYEKAKHLQ
                     IPVLMIHGDADGIAEARGSIELDNHIVSDKKTLKIYPGLYHELMNELPESREIVLNDI
                     KKFLDSVVPEKL"
     gene            complement(44340..44954)
                     /locus_tag="PLF66_04985"
     CDS             complement(44340..44954)
                     /locus_tag="PLF66_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002747769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HMZ63427.1"
                     /translation="MVTSCNKQDSNPTAEMKPDNRKKIIFFGDSLTAGMGLLNPDESF
                     VARIAVKLKEDGFDLKVINAGLSGDTTSGGLARLDWSISKGVDYFVLELGANDGMRGI
                     ETELIESNLKKIISKVREKNPNVKILLVRMLTFPNLGPAYVKKFNAIYPKVAKEEKIP
                     LSAFLLEKVAGIKELNQKDGIHPTGEGHQLLAETLYPSVRKLVE"
     gene            45088..45942
                     /locus_tag="PLF66_04990"
     CDS             45088..45942
                     /locus_tag="PLF66_04990"
                     /inference="COORDINATES: protein motif:HMM:NF013589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HMZ63428.1"
                     /translation="MNKSDSYLVGILPEFNFRFLLTDCSNSVQEVITRHEMKYSSAIL
                     VGKTMLASFFLAGLVKEETTISIQLEGEGDIERVMAYSDRIGRMRGMAKHNAIQAEVS
                     DHTLGIGKGIFRVTRWGGVQKLHQSITKLDDSAFETNLLNYINESDQLISFLSIYLDM
                     DTKPIRACGMILQALPFTTSRQIDDLMDKISNMNIEQSKIFAGSIDESLKLMENMLET
                     KADVLETGIPEFYCGCSLDKIKGVIASIGKEEAFSILEERGSIEMICEFCMEKYTLDA
                     EEVKLLFI"
     gene            45947..46375
                     /gene="tsaE"
                     /locus_tag="PLF66_04995"
     CDS             45947..46375
                     /gene="tsaE"
                     /locus_tag="PLF66_04995"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HMZ63429.1"
                     /translation="MQLLYTLSDIDKPIQLVRNYLKEKKIQHPVILLTGEMGAGKTTF
                     TSNFVKSFSPNLNPNSPTFNLMNEYKTSSGSIFHFDLYRLKSPLEIENLGFEEIWGKQ
                     GLSIIEWWQIAKDYFDSSSLEIKIELADNNTRLFSLISKS"
     gene            complement(46493..47014)
                     /locus_tag="PLF66_05000"
     CDS             complement(46493..47014)
                     /locus_tag="PLF66_05000"
                     /inference="COORDINATES: protein motif:HMM:NF019235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1564 family protein"
                     /protein_id="HMZ63430.1"
                     /translation="MNHDIKIFSNPKISFPRGEYIYSEFSSTLLIPNRYYDIFLEKCS
                     VYGGVRAYVNFLLSKYQLFITNGLVPGYSNVTTKYQEKGQNLQKIAFRPMPQDWAELK
                     LYRVSFGMSISAFLVYLLIADSVDFVKASSAHLATVGISEVSKFDLYAKVFLTNNRSD
                     YTIVFQYRKSRFG"
     gene            complement(47211..47750)
                     /locus_tag="PLF66_05005"
     CDS             complement(47211..47750)
                     /locus_tag="PLF66_05005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63431.1"
                     /translation="MILVKYIRDFIFFTPIAIIGWFKVLFHFLIFLFSLWKQFTFFKQ
                     VTIIFFTVQLIFATRPWFEYTINFIGTDEVWAVSSKINLFFIFISLINLFLLFSNSSI
                     SNILILLSEVIMAILFVVGYNSPTSVHIDFIKNSDYRYNTNFYIFGIALIASLLVSIR
                     NFLKERNSNNPREELVDSN"
     gene            complement(47747..48205)
                     /locus_tag="PLF66_05010"
     CDS             complement(47747..48205)
                     /locus_tag="PLF66_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002974326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HMZ63432.1"
                     /translation="MPIEEKIKSLGLTLPPVPKAIASYIPANGSGKLVFTSGQLPMKD
                     GKLLYTGQTGKDVTIEQAREAMKQSCLNALSAIKGYIGDLDQIQKVVKIGAFVSSAES
                     FFEQHIVANAASDLLLEIFGEPGKHARFAIGVNTLPLNACVELEVTVEVK"
     gene            48286..48993
                     /locus_tag="PLF66_05015"
     CDS             48286..48993
                     /locus_tag="PLF66_05015"
                     /inference="COORDINATES: protein motif:HMM:NF014373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HMZ63433.1"
                     /translation="MVATDILDIVTKLFLAAFFGGVIGFERKFQHKPAGLRTNMLICL
                     GSSLVMILSQHLSYTVSESDLLTGRTMQKTVGDPARLAAQVISGIGFLGAGAILHGRA
                     NGTIKGLTTAATIWTNAAIGLAVGAGYYILAGISTLFVLFTLYVIRMIENKTNSKHPR
                     PRTLLIILKKVKKISQLKKEIYRKGIFIDSESIKKRMDEVEYRADLNISPVMEEILLN
                     HLNEDDNILSYNITSDQ"
     gene            48994..49782
                     /locus_tag="PLF66_05020"
     CDS             48994..49782
                     /locus_tag="PLF66_05020"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008593428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HMZ63434.1"
                     /translation="MSIFKITDHPKFPNYYKYCENTGTCFYIPAKERSYSNSYFMEEY
                     KAQYKKTYYEDEQSLRSLAKRRLAVLEKFNRPRKTKLLEVGSAAGFFLDEARKIGYNT
                     KGIEISESEVNYAKDKLGLDVENISFLDFDAKNKYDCIALFFVLEHFPDQETVLNKIF
                     SLLNESGYLFLALPSIYGPSFSTNPEEWFKTHPEDHFADYSPFSLKKVFQYFNAKILY
                     CEPMSYHVSRDKGMRGKFPFRFFYNSLARLTSYGDTIQILAQRR"
     gene            49785..51398
                     /locus_tag="PLF66_05025"
     CDS             49785..51398
                     /locus_tag="PLF66_05025"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004461183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HMZ63435.1"
                     /translation="MRFDELNIHENMLRVFQEIGYVELTPIQEKSIPYALVGRDLTGL
                     AQTGTGKTLAFLIPIVNKILNENPEGPVALILAPTRELVIQICEEAKRLLKYSDKKVA
                     SFIGGTDYKEQLQDIEDKTEIIVATPGRLIDHIKSGKLSLSQIKFFVLDEADRMFDMG
                     FIKDIRYVMKKCPDDKQVMLFSATLSYYVIRLASEYLKDPVEVSIEADKVVTDNIDQK
                     LIHLGRDEKLPYLVNLILKDQEDGLGIIFTNLKIMVPEIVHKLSKYGIPSTGISSLLD
                     QKKRVRLLKDFKFGKYKYMVATDVASRGIDVDNIKVVYNFDLPSDTENYVHRIGRTAR
                     AGRKGRSISFCSEKDYIELEKIEKFLGTKLETISVDEEYLNYPEGEFTPFVGEKLKGA
                     SPNQNKQQRHPNQKGKGNNFQKKGDRYRDDKDRKNKGKNEKFKKDKYKPKQEPMNPLD
                     EAKLYLQKADSMLGTEKSKTSTHPSKKETHPHSKEKVHKIKSEKPYDPKKQPYDKSKR
                     NLFDINEQNNKDGKKSVSIWKKIKSFFGR"
     gene            51365..52474
                     /locus_tag="PLF66_05030"
     CDS             51365..52474
                     /locus_tag="PLF66_05030"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HMZ63436.1"
                     /translation="MEKNKIIFWTIVLISFLVTFVSIKAETINVEKGYVRFNDLRKKL
                     PGLQCKLDTVILVGEIRFKKSTEFKFRVDSAYYIHKNNIESLPKPILYKMGDLLLPKE
                     MVEAIFIFLIDTEVSYKITDDRLTLNLYEKVEIKPEEIPLRHLIIDAGHGGKDPGATA
                     VNGEYEKNYTLKIAKMVRKYIKKKFPNLKIKFTRKKDVYVPLEDRAKIANKKLQTSRD
                     TIFISLHCNSTPTPEKSPHGYEVYYLAQSAPIEINRESSILSNQLIDVRRDPKVQYIQ
                     SSMLSSVIQRRSILLAKSIDNKLKESLDSKIASRGVKKNNYQVLRGSMMPAVLIELGF
                     ISHPQDVKMLEEKAVQLKLAEGIAEGIKNYVMAKN"
     gene            52455..53231
                     /locus_tag="PLF66_05035"
     CDS             52455..53231
                     /locus_tag="PLF66_05035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63437.1"
                     /translation="MLWQKIKSIYRASIDFCKNLLSKIFSEAVGETKSNKFRFFFISS
                     SAGILFFIIFILVGKNPFSLLVPFYFYDLPSIDKRTEITLFISDGKNNLLKSKRKVLL
                     AENDLRRNLNIIIGELSQAPYEDVIETTEENVFPKKLPNIRNPILISWFLESEKKLII
                     DLDEEVIQKEISNIRIRVDANDDDLDEKQKKEEEELEKKRYAELQVAKMKILNTTLQA
                     LEKTIFANFPSIESIEFKINGHSKELAGLEYKLSEIKKRN"
     gene            53233..53943
                     /locus_tag="PLF66_05040"
     CDS             53233..53943
                     /locus_tag="PLF66_05040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63438.1"
                     /translation="MSNLQQFPINLQLYKKRILIRAILIFTVNLALLGLTVWSAPSES
                     RLNYLAITGIVITAIGLFLNKNYERQVSILKDNYLELNNHILQWYTGNGKCTTINLKR
                     VKKIERDKYRSFDRFLIFEGENPEIILNLLEPDEFQNRIEEYTKLKTQIFIIPWRSYL
                     IKGVSFFIPALIAFGFYKFQFLELKLFFLILNMNAIFFLVHFSEKRTRGGFAEATVRR
                     SSIILFLLLAYQLLLLFG"
     gene            complement(53956..54483)
                     /locus_tag="PLF66_05045"
     CDS             complement(53956..54483)
                     /locus_tag="PLF66_05045"
                     /inference="COORDINATES: protein motif:HMM:NF019235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1564 family protein"
                     /protein_id="HMZ63439.1"
                     /translation="MKENKNGKTFSNQKFEFQREDYKYSEFSSTLLIPECYLQMFQLK
                     CKAYGGTREYVTYLLSKYQIFITNGLVPGYSNVTTKYQEAGQNLHKIAFRPNPEDWAE
                     LKLYRISFGMSISAFLVYLLIADFVEFEKAASSYLVTVGIPVISNFDLSTKVILSNNR
                     SYYTIIFRYRKNRYS"
     gene            complement(54647..55738)
                     /locus_tag="PLF66_05050"
     CDS             complement(54647..55738)
                     /locus_tag="PLF66_05050"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015681040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HMZ63440.1"
                     /translation="MNTKSKPLTKEEIEAFGKELESIRTEVLAKVGQEDADHIRRIQA
                     SYRYNEIIGRLLIHFSLDPITFGLGTSLLAISKILNNMEIGHNVMHGQYDWMNDPEFN
                     SNKFEWDIVSDSAQWKFYHNYMHHTYTNVIGKDHDYGYHFTRLSSEQPWKITNLFQTI
                     ANVYLAFTFEWGVGGHGYKVEYLEKPKKQRTKRTFQEYKDVFNKKAELQLFKDFVFFP
                     LLGGFMAPKIFMGNLFANVIRNLWTYSIIFCGHFTENAETFTPKDIENESKAEWYLRQ
                     LKGSSNLEGSKLFYLMSGHLSHQIEHHMFPDIPANRYEEIAPRLQDLCEKYGQHYNTG
                     SFVSQFASVWKRIFAFSLPDPFAEKVMAA"
     gene            complement(55757..56836)
                     /locus_tag="PLF66_05055"
     CDS             complement(55757..56836)
                     /locus_tag="PLF66_05055"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding domain-containing
                     protein"
                     /protein_id="HMZ63441.1"
                     /translation="MKTFPLKYNKMQEYKEYFNPKDWIEFVLREINPMFSTSKVKGRI
                     LSIHLETETARTFTIETNRKISFKAGQYIPVTVEVNGRRLTRYYSISSAPSEGLLQIT
                     IKRQKDGLVSNFMHDHLSLGSVIELGEAKGEFVLTENLPEKLLLIAGGSGITPIYSIL
                     KTLSKMGYTGEATLLYFSRSKEDVIFHEAIQNLEKNNSFLKTHFILTDIESNGFQFGF
                     LSEKMLKTLVPDFDLRTTYLCGPKSLQDSVKDLLPKDKIISENFQPFLKSSAKRHLKD
                     VSVTLTKSHRTILVKGEKNLLEELEDNGIYPQSGCRMGICHTCACTKKTGTVTNLQEG
                     TVSENKDETIQLCLSRAEENLELDL"
     gene            56863..57609
                     /gene="fabR"
                     /locus_tag="PLF66_05060"
     CDS             56863..57609
                     /gene="fabR"
                     /locus_tag="PLF66_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012390030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HMZ63442.1"
                     /translation="MFTNIYILFPSSSQKKLFFSERAKKVKEQMKLNQRYQQKLRTRT
                     TLIQAALKLMGEDKSLGSLSLREIAGEAGIVPAAFYRHFKDIEELGLALVDDMSVKLR
                     VILRDARKKGAYKTALQVSIELFFNYVKNNRLLFRFITRERVGGSRRIREAIRNEMSF
                     IASELASDMKRPGMPFSKLEFISDFIVTTAFTLAGDYLDSDPNDQATGKKLKVKSIKQ
                     LRLIFRGSLITKRRIKKRINPEQVNENDLR"
     gene            57596..58453
                     /locus_tag="PLF66_05065"
     CDS             57596..58453
                     /locus_tag="PLF66_05065"
                     /inference="COORDINATES: protein motif:HMM:NF013246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family ion channel"
                     /protein_id="HMZ63443.1"
                     /translation="MTSDDRKAFLELAKLSIPYLLFSLFYYSGIIFLEEKFGDHIYKI
                     SGQMGTVFGLALAFFLGFRMNTAYDRWWEARKILGELVNTSRSFAAKVVTYFSKDTNA
                     QKVLIGKLLLAYIHQLKDQLKQNSNIKNYSHLLDGKEKDLSQKSNPAVQLLKEISMSI
                     EKNWKTKTDLDKNDLMQHISRFYETQGKAERILNTPFPRIYKSLTNVMVYIYVLLIPF
                     FMGDIDLGGEVSHWEYLSIPIMSLIGSIFLTVNRMTNLYADPFSNHVISLPIDKITST
                     IEKDIIQML"
     gene            58450..59178
                     /locus_tag="PLF66_05070"
     CDS             58450..59178
                     /locus_tag="PLF66_05070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63444.1"
                     /translation="MNTSSKLLIAFVFSISLFVGIYFLKPMINTFLHPQKTVEMVIEK
                     LKEEREKEFLESYPDLYSLHSIRLITVKEKQILEVWSKATEKDEFILRKTYPYTSFSG
                     VLGPKLKEGDRQIPEGVYSIEGLNPNSKFHLSIKVGYPNDLDKKLALEENRESLGGDI
                     FIHGKASTIGCIPIGDSNIEELFYLVATVKPIHSSIISMPVDFRLTENNRYIGNTNVE
                     KEIYKSIRQALHHSYTNSKTFLNR"
BASE COUNT        20224 a        10351 c        10025 g        19097 t
ORIGIN      
        1 caatatgaga agcaatttgc ctaaaatcgt gtccactaaa tatgtagtag ttcaaagttc
       61 gtgactgaac aaaaatgtgg cgaagcccaa gaccccgaat cttctctcgg ggtcgcagct
      121 catcacgctc ctgttgtgcg gtgtgaggta gcgccttcat aagatttctc tataatatta
      181 ttaactctca caaaaaaact ttgtaatact ttaaattgat tatccttatc tgaattcaca
      241 aaactttcat attccttttg actcatataa aatgttatcg cataattatc tattttataa
      301 ttctcatcta aaatattatt tttgtccttc atcaaaaaat cattaatagt tttattccaa
      361 tcgtgatcca accaaatgca tattgggttt tcagtgttaa aaatgattcc aatagctgaa
      421 tatgaaaata ttttatcatg tttctttaga gtataatatt ttcctaagta atgattttca
      481 taagctctac tttgcttgta ataactttca catttatata tttcttgatt tgaacagtta
      541 ataattacat caagcaaatt gttaaaataa aaaatagatt ttaaggcatt agcttgaaat
      601 ctaattaatt ctatttcttt aatcatacat accttcttta gatagttaat aaaatctaat
      661 ataattggat cgagtttttc tttttctaaa gaactaataa aatctttcca atataaaata
      721 gtccaaccat atttttctcc aattttatag ttgtaattat caattgtatg tgctgaaatc
      781 aaaattttta ttgagtttgg gtcatcgatt aaatttgagt attcttcaaa atgataatta
      841 ccaatatcaa atattttatt ttctataaaa tatttttgct tatttccttt atataaataa
      901 aaatcaattc gtttgttatc attcggaaat atatattctc tttcgatttc aaatccattt
      961 gatgaatctg ggcatttaat aaaattcaaa aaataatttt taaactgagt attgctttta
     1021 aataaagccc atacgaaatc cgacaaatca ttctctttat aaagtctttc tgataaaatt
     1081 tctaaaaatt ccataatatc tatttaatta cctcataccg cacaactata cttctccgaa
     1141 tttattatac cctgtcaata ggattatttc gtattaactg attcatatgt agttaatatg
     1201 acaaaaaact gaggatatga tgtttgacta agcaagagcc ttttcaaaaa ctccccacta
     1261 gcaaaaccgc aaaatacaac tcaacagaaa gccaccatac tccttccatt accaccaaag
     1321 ccttttcagt atatcaaaga ctataccaac actatacgaa ccactatacc aagtcaccag
     1381 taagtgcgta gtcaatacaa accatagaaa tccttttttc aattccattt caatgaatat
     1441 aaattttaaa aagctcatga gtgaaatggt agcagagtta aataaaagag atattagcaa
     1501 aacaaatact tatcagtgat aataatatac gataggtgaa atttttatga cagatactcc
     1561 cgattaaaaa tctcgggagt gaaatcttac atcacttgca taattcttaa atcaaataca
     1621 cgcaaacaca caacgcattc aatttaactt cttcgtgtct cttcgtgcgc ttcgtggttt
     1681 ataatgagcg gagtcgaagt atggtagaga aaacataacg atatagatga caggttgcat
     1741 gacctgccct acattttttt atataataac aatccaaatc cgaaaccatc gaaccccatc
     1801 ggtgtttatc gtccctatcg gtggaaatct tcaaaccccc aaccatcggt gcctatcggt
     1861 gttcatccgt gtttaatcca cccttcgctc caagtattcg cgaccaacgc cttcgcaggt
     1921 ggtaaattct aaatacaatc ttatttttac gaactagact cttgtgcaag atcgagcatt
     1981 ttttcataaa gtgaatttgc gatccaataa tttttatttc gtaaagaatc caaatcagaa
     2041 cgaacggaag aaatgatttt taaatgcttg gctcgaagta atacagtaaa gaagccaatc
     2101 acgtttatcc ccatatttaa ggcaacgttt cgtcctaatt tttcatctat caaaagattt
     2161 ttcttttcaa aataggcgag agcaattgca ctctgttctc ctttatctaa atgcgaaatt
     2221 ttttcgagaa gctcgttatc aataacagga ttttttacta ctttaataaa ggattgaaaa
     2281 gcactctcta aaaattctcc ttcctctcca gctttagcta acaattcttt ttctacaatt
     2341 ttcggaatcg taatctcagt aaagaccaag gggagtaagt ttagacgatt aatttttgca
     2401 agagcgacga gtggactcgt attagaaatg attttcatgt tttaaataat tcaatttcat
     2461 tttctaattc ttgttgagta aaatctataa caggaacttt ataagatccg cacacatcta
     2521 aaaactgaac tcgagtcatt ccagctaact ctgccgcttt accagatgaa agtttattta
     2581 actcaaacat tttaattgct gccattagta gtatctggga tacaaactct ttctctgtca
     2641 tctgaactga gtttaaaaca tttaattcgt ttcgttctaa tagcataaat tttctccttt
     2701 gttaaagtaa attgtcaatt atcattcatg tcaagccaga atagatagat tattaataca
     2761 ataaaaactc atattgactt tttaaaactc cttcgtgatt cttcgtgcgc ttcgtggttt
     2821 ataatgagcg gagtcgaagt atggtagaga aaacataacg atatagatga caggttgcat
     2881 gacctgccct acattttttt atataataac aatccaaatc cgaaaccatc gaaccccatc
     2941 ggtgtttatc gtccctatcg gtggtaatct tctcactctc ctatcaccaa acttatccta
     3001 aacttagggg taaagatctc ttaaacttat cctaaattta tctccgaaga tatctttagg
     3061 ggaagtttag tcctaacttt aagggatgtt tagtttatcg ttaatagatt ccattcacga
     3121 tttgttcaag taacatcttt agcagaagag attctcgata gaagatatag agaatgacat
     3181 ttaaatccac ttgcaaaatt tttaaataaa atacacacaa acaaacaacg catccaaatt
     3241 atccaaatat cctttgaata aaaacccttc gtgcgtcttc gtgtccttcg tggtttaaaa
     3301 aaatataacg atatagatgg caggtctcga cctgtcccta caattttaaa caatcataat
     3361 acaaacaacc aaaacccatc ggtgttaatc ggtggtaatc tttaaaccct aaaacccatc
     3421 cgtgtttatc cgtgtccatt ccacccttcg ctccaagtat tcgcgaccaa cgccttcgca
     3481 tgtggttaaa attttttaat tctcatttcg ataaatcgag atcacagtaa cgtcatccga
     3541 aaactgcttc gtttccacat attgaaacac atcttgaaag atggagtcgt ggagtttttc
     3601 aaattctagg tttggatttt catttaaaaa atcttcgagg cgttccatgt tgtaggcttt
     3661 gccagtatct tcattcatgg tttcgagaag accatctgtc atgatgagta gataatctcc
     3721 ttttaagaaa ggaatcgatc tttcttcata gagagcgtta ggaagaatcc caattagttt
     3781 tcctgcagat tttaactgta aacaattacc agattctttt ctaaatagaa ttggacttgg
     3841 atgaccaccc ttcgcataaa aaagagattc tttttctaag tcaattacgc aataaaaagc
     3901 agttaagaaa tatccgccca ttttatctgc taagcattga ttcagataga ttaaaaaatt
     3961 agcaggactt aaaaatagat ttttctcaaa gctttggatc acagtatgca ccatcacagt
     4021 aagaagtgca gacgaaagcc catgaccgga cacatctccc actaaaactc caattctatt
     4081 ttgatcgaga ggaaagaagt tgtaaaaatc tcctcctact tctctaaaag gaatgtattt
     4141 agatcgaaca gtgagattat gaacgatagg cacatctgga ggaataattc tttcgattag
     4201 ttttctagcc cgaatgtttt cttctgcgga aagattcatt tcgtcaaaat acttagcatt
     4261 tcgaatcgct agagcaagac gttccgaaat cgcgcaagtc aatttcaaat catttcgatt
     4321 gaaggaataa ccgtcttttt tattgtttat atttaaaaca ccgatcaatt cattttggat
     4381 tagaagcgga acagagataa gagaattatt ttcataattc gctctttttg aatttttaaa
     4441 tcttggatct gtatctagat tttgaatgag aagtggttct ttattttccg caacccaacc
     4501 agcaattcca tctcccggga agattttaat attttttaca acgtcaggtg gaagcccctt
     4561 agacttaaac attttaaggc aaccttcttt ttgattccac aggagtatag agccagactt
     4621 tgcctcaagt ccactcaaac aatgctcgag taaccaatcc cctatttcat gaatatttac
     4681 atttgtgcta atttccttat cgaagtcata caaaagagag agctccataa ttctctttct
     4741 taaattttca ttgagtgcac tattttcaat agcgatagcc gcaagatctg ccaaatattc
     4801 taatagttcc aagtcatctg aattaaaagc ctcattgttg tttttattca tgacttctaa
     4861 aataccaatg agcttgtgct tttgatacat tggaacgcaa atgattgagc ttgttttaaa
     4921 accagttttc tgatcccaac tagaatcaaa cctaggatcc ttgtatgcat cctctattaa
     4981 cagtggatga cctgcttttg caacccaacc tacaattcct tcccctaatt cgattcgaat
     5041 gtatttctgt aaaacttctc ccttttctcc tagtgccact tcacaataga gatgattatc
     5101 cgatggatca aagagaaata aagaagatgc ttccgcctct agtaaatctt tagcatgaag
     5161 cataataatt ggtaaaagtt gaaatatatc tagattacta ttgagagatg tgctaatagt
     5221 taataaagtt ttaattttat tggtcttcat ttatttcccc ggaatttaaa ataaatagac
     5281 ctagggcaat ggcaaccaaa ataacaatct accaaacgta taaatttcag acaaaactca
     5341 atctttactt tgtttcaggc aaatcatgat aaggggaaac aaagatacct gctccctttg
     5401 gcttagaaag ttttccatct tttccaaagt ttgcaataat gtttttaaag gttgtaattg
     5461 ttttttcttt ttcctgtttt tgttttttat caggaagaga ttctacataa agacttgtag
     5521 ttggatacgc aaaagtaatt cctacttctt ttgccaaacg cataacgtct aataaaatca
     5581 actgtcttcc ttctaaattc tttggaagag aatctaccgc aaggcaaaat tgtagataga
     5641 taacaaggga agatgctccg aaatctctaa aaccaatatg aactgtatct gcaacaatca
     5701 aaggattttt cattatgatc tctttgattc cttctaaaaa tgcttccatc ttttcaggat
     5761 ttgtatcata cgttacatca atattaataa cgtttcttct ccatcgtcta acgcccatat
     5821 tttctatatc agcatttgca acaactgaat ttggaattgt aacaagagag tcttcccaag
     5881 ttcgaatgag agtcgaacga aatccaatgt cttctactat tccttctttt ccatttatta
     5941 tgatatgatc atcaatttta aatggcttgt ccataaatat cattacagaa ccaaataaat
     6001 tggcagctgt atctttagct gcaagtgcaa gtgcaagacc gccaattcca agaccagcaa
     6061 tcagccccac tacattcacg cctatatttt gaagggcaat caaaaatcca acagtaacaa
     6121 ataggattcg gagagtctta ctcattaggg gaattaattt atcatcgatt ggatttttag
     6181 ttcgcacaga attatctttt aaaaggtcaa cagcaagatc tgataattta taaagaaagt
     6241 agataaaatt aagaccaaga catactttta aaataaaatt aaatgtagtg aatatttttc
     6301 cttcaattcc agaaagatat aaaaatgaaa accaataggc aatagaaaag aaataagcaa
     6361 ctggtcttgc gagtgtcgtt agtataaaat catcccaacg agtgcttgta tttcttgtta
     6421 atttgatagc taatctaaaa aagaatttag atacatattt gagtaaaaaa ctaataatag
     6481 tagctaccag aagaccgagc cattgccata taaaaaagaa tccccatttc tctcttgccc
     6541 atttcggaaa aattgtctcg agccaaggaa gagaataagc agctccacca cctgtctttg
     6601 caagataagg catatggctt gtctttcgat agtattcact agcactcgct accgtttctg
     6661 cggtaaatac atattcaccg cttctattcc cgctagtaat ttttgttatt gttatttctg
     6721 tatctgggat actccattta tcaacggctg tctcaggaat cttttctaat tctaagacat
     6781 aaactcgatc aattgcttct tttaaaaaga ttgctgtctc ttttcctttc tcttctctca
     6841 caagaggagc aatttcactt agatctaaac attttataga tttttcaatg gctaattttt
     6901 tactctcatc gtttttctct actccctttt tatattcatt catagagctt agaaacatct
     6961 tcatagtttc acgaggggaa tctaacttca gcggagaaag aggattctct gcgttagccg
     7021 atacaataga aataaaaatt aaaaaacaaa atcgaaaaaa catttgaact ccttaaatta
     7081 aatagaatca tttcattttt tttcttaaat ccatttgctc cagggaacag ctccaaaatg
     7141 ataaagccca gaccaaattg taaatgccat accaaaagaa cctgcaaaaa aacaaaagag
     7201 ccaatgcttc cactcgatat gttcatccct aagagcaaga taggttaagt atagataaat
     7261 aggaaaatat aaaaagatag cagtgaaata accaggagaa tacgcattga atttaaattg
     7321 agtgtaaata tgaaatacga aattccaaaa ctgttgacct gaaacccata aaaacaataa
     7381 aaaagtagaa agtctatttt gcttttttgc agcaaacaat gtgagtccga tcaagactac
     7441 catgaacatt gcattgttga tataaaagag gctaatttcc attttccctt ctagaacata
     7501 tgttacccag taaggaaatc catttgattc ttctatgata tgaaagaaaa atgcaatagg
     7561 aaagagccag attaattttt tatagaagga atttttttca aattctagca attttgttct
     7621 cctcttccat tttcggtgga aataaaaact atttcagaat tttttattta gatacttaaa
     7681 gagacatcac tcaatttagt aaaaaaggat taccaccgat gacaccgata acagaacgat
     7741 aaaaaagaga tgaaaatgaa agtatcgtat cttttatcgg tgtttgcgaa gcgatcggtg
     7801 tcctcggtgg tagtctttac gagttgcacc gcgcttcctg cgactcaatt tttcagaatc
     7861 taatttaaga ttattctccc ttaagctccc ttgacattaa gttttctacg gtttgttttg
     7921 gatcttggtc ttcgtataaa atacgataga cttgctccat aatcggaact tctactccta
     7981 attttttact gagtagatat gcacttttag aagtaagaac tccttctgct accattctca
     8041 tatctcctaa aatatctttt agtttttctc cttgcccgat tttaaaacca accgttcgat
     8101 ttcgagataa gtctcccgtg caagtcaaaa ccaaatcgcc cattccagca agaccaagca
     8161 tcgttagcgg atttgctttt ttctgcgttg caattcgatt gatttcacca agccctcttg
     8221 taatgagtgc cgctcttgta ttatatccaa aaccaagacc atctgcaata ccagctgcaa
     8281 ttgcaatcac gttttttaga gcaccacaaa gctctactcc aataatatca gaagttgtat
     8341 agcatctaaa tcttttgtca gacataaaaa cttgcacatg tgcggctaac tttaagtttg
     8401 cactcgcaat ggtaacggca gtcggaagac ctgaagctac ttctttcgca aaagaaggac
     8461 cagagagata agcgagttga tgatgaaatt tttccggtaa aactctttcc ataatttctg
     8521 atggaatttc gagtgattca ttttcaatcc cctttgagca gctaacaatg gtttgcccat
     8581 ctcgaataaa gggagttgcc tgcttcatca ttctagcgac atgcggcgta ggcatcacag
     8641 aaaggatcgc ttctccaaaa ttcatacatt cttctagatc attggtcgca gttaaatttt
     8701 ctggaaatgt atatcctttt agaaaaactg aatttgtatg attctgattg atctcttcta
     8761 ctacatcttt ttcatacgcc caaatttttg taggaaaccc ttttcttgca acatggagtg
     8821 caagtgcgtt tccatatgat cctgccccaa tcactccgat ttgttttact ggtaacattt
     8881 atttctcctc tcgaatttac attcatggaa gaaatagaaa aaaaaggaaa gaaaaaaaga
     8941 gagatcgatt ggatttcaga ttgacaaaag tagaaattac accatgttaa atcttatgga
     9001 aaaagaaaaa ttattaaagc gcatcacaat aaatccagaa caatgtggcg gaagaccttg
     9061 tattcgaaat atgagaatca gagtttcgga tgttattgat atgctttcgg aaggaatgct
     9121 tccaacagaa ataattgatg attttcctta tttagaacta gaagatatta aggcaagcct
     9181 tctatatgta tccagatata tcaatcatac ggtcattgca gcttgatcct ttggattgac
     9241 gcacaaattt caccaaagat agctaaatgg gtggaagata ccttttcaat caaaacatat
     9301 catatatctg aattcaaatt aacacaaacc acagacagag agattttttt aaaagcaaag
     9361 cagaactcaa cggatgagac aatctgtatt ctaacgaaag atattgattt tgttttgcta
     9421 cttgaacaat acaaagttcc tccgaaaatt atttggctca catccggaaa tgcttcaaat
     9481 gaaaaaatta aattaacttt aagtaaagca ctcaaggaag cattgcattt attgaaccaa
     9541 gaagatttga ttgagattag ataggtaatt taatttatat atctaaattc aggacggtat
     9601 ttcccatctc gataatagaa gaaaattcct tcatgccatt ctcgcttttc ttctaaacac
     9661 cagattcaat ctttatgacg aaagattttg ccgttacgag aagttcttcc cccatattct
     9721 tctttcgtgt tgtatgtatt aatgcaagtt ccatcggcat taaacttata ttcatagtta
     9781 ccatctactc catcgaattt aatttctccc ttaaaacaat ctaacgttac atattcttta
     9841 ttattcccga gaatacattc ttccccctca ggaaaaaatt tatcttttat taaaagaact
     9901 gtataagttt tatttttggt aacaattcct tttataccga acgccaaaat taatttccct
     9961 tttttaaatc gcaaataagt gttcggtcta aacacttcct agtgttcccg cacttcagta
    10021 tgtgcggtat accaaatgct atgaaataaa atccctctag aaagtttctt ttgtcatttg
    10081 gtatatactt tccaatgctt taactttctt aaatccaaga gagtaatacg tttctctgag
    10141 agaggcacgg attaagactt gtaaggattt atcaggcttt ttgcctttcc aatttgtcca
    10201 aaataaattt aagtatgctt cgaaagccat ttttagaatc ctctatttta tttttcaaat
    10261 agaattattt agaagtcaag tagttttgaa tttttaaact gagatttttc ataattttat
    10321 attaccactc agtatataat tcgtataata aaaatatttt tattttgaat atagacttga
    10381 ctcatttact ttcgagtttt atgaaatgtt aagcgaggca acaatgactg atacaactgg
    10441 aatccttctc aatttcacag aagagcaaat gcaactacga gaactagtaa gagacgtagt
    10501 aaaaaaagaa gttataccga atcgaatgta ttatgatgaa cacaacgaat atcctagaaa
    10561 agttttagac aaattcaaag aagcaggtct ttatccagcg atgtttgaag aagaagcagg
    10621 aggtctcggt ttagggattc ttgcgcaaat gattctagtt gaagaagttt cttatggatg
    10681 cttaggaatt aacgtttcct ttgctactac aaaactagga gctctcccaa ttcaaattgg
    10741 gggaaccaaa gaacaaaaag aaaaatggct cgcacctctt gtcacaggag ataaaattgc
    10801 agcctttggt ttaaccgagc ctggggcagg gtctgacgtt ccaaatatgg gaactactgc
    10861 ggttaagaaa ggcgcaaaat acgtgttaaa cggaacaaaa caatggatca gtggcgcagg
    10921 gcaagcagac atctatactg tatttgccat tacaaataaa gagagaggaa ccagaggaat
    10981 ttcttgcttc atcgtagaaa aaggaactcc tggattctca ttcggcaaga aagaagataa
    11041 actcggaatt cgttgttctg aaaccagaca acttattttt gaagattgtg aaattccaga
    11101 agagaatcta attggactca aagaaaatac cggatttctc actgccttaa aaactctgaa
    11161 tttatcaaga ccagcggtgg ctatatcggc tgtcggtctt tcccaaggtg catttgactg
    11221 tgcaatccaa tactctagag aaagagaaca atttggagta aaaattggat ctttccaagc
    11281 aatccaacat ctactcgcag atatggcaat gcgaatcgaa gcaagtagac tcatgactta
    11341 caaggcagca cttctttgcg aaatggaaca caaagacatg gcaaaatttt ctgctatgtc
    11401 caaatgctat gcggcagaca ctgcaatgtt tgttgcaacg gaagcagttc aaatctatgg
    11461 aggctacggc tacacaaaag aatatcctgt cgaaaaatac tttagagatg caaagatttt
    11521 acaaatctat gaaggaacta gagagattca aaaaaatgaa atcgcagcag gactcataaa
    11581 agaaagtgca acaaggaagt aagtatgaaa aatttatttg atgtaagcgg caaaacgata
    11641 ttaatcacag gggcaagccg aggaatcgga aaagctttag cggaaggatt tagagatgca
    11701 ggagctattg tctatggaac tggaacaaaa ccggaaacca ttgcttggat gaaagaggca
    11761 aacattgaag gaagagtaaa caatcttctc gcagaagata aaatcaaaga catcatcgga
    11821 gaaatcaaag ccaagcacgg gaaattagac gtgcttatca ataacgcggg aatttctctt
    11881 gataaacctg gctctcattt aaacgaaaaa gagatgagtg atctaattga tatcaacttc
    11941 aaggcagtct ttcgtgcaag ccaagcttat tataaatcac ataaattact aggaggcaac
    12001 attatcaaca ttgcatctgt attagggctg agaggtttta ctcttgctac agtctattct
    12061 ggaacaaagg gagcagtaat ccaactaacg cttgcttgcg cagcagaatg gattcgaagt
    12121 ggattccggc taaatgctat ttgccccggc tttatcgata ctgatatggt tggaaatatg
    12181 aaatccaaag aagcgctcat ggaaggaatc aaatctcgta tccctatggg ccgcatggga
    12241 aaaccagaag aacttttagg aactgcgatc tttcttgcga gtgacgcatc tagttatatc
    12301 aatgcacaga ccatcgtagt ggatggtggt atgacaaata ttgtataaaa ggattaccac
    12361 agatggacag gcgaaggcgc tggtcgcgaa tacttggagc gaagggtgga tgaacacgga
    12421 tattattaga gtgattgaaa agttaagata tacaaatatc agttcgagcg atagtcgaga
    12481 actgcaacag cgaaaaaact ttattttaaa ccttcgcgat tacactcgaa ttgcgatgat
    12541 tcatatccga aattttccat tgggtatctc taaatagaaa cactctcatg aaaatactcg
    12601 ggttcatagc tatttccttt tactttttct atgcagggta tagctattcc aaaggaagtt
    12661 tatccaatgc gctttggatt tgtcatattg ggtgctttct tgtaggctta ggactattag
    12721 ccagtatcaa catcctgagc tctattgggt ttttgctact atcggttgga aatgtgggat
    12781 gggcacttta tttgttagcc ggtggagaat taattctacc ttctatccta acgcatgtag
    12841 gtggattctt aatcggtctt tttgtagttt ataaaaatgg aattgaagct tatacctatc
    12901 tctattcctt aattgcattt attcttttac aaatcctatc tcgcctatcg acagatgcaa
    12961 aagaaaatgt aaacctagca tttcaaatcc aagaaggatg ggaaaagatt ttcccttcct
    13021 atccaatcta tttacttgtt atctacctac ttcttttctt tggatttttt ggaatcgaat
    13081 ttctaattca aaaattaaaa tcccaaacaa gaatcgaaga aacaaaattt aattcggaac
    13141 cacaatgaaa aaatttaaaa tctttctatt ctttctaatt ctactcgtat cctattttac
    13201 gtttaagtct acgcctatcg atccagttgc ctttactcca cctgagccaa gagaaatgaa
    13261 tggacctctt tctaaaaatg aaattctaca aaaagctgag cttttaggac tcagaaaaat
    13321 acatggacca gaaggacttg caatcgatag aaatggaatt atctatggag gcacagcaga
    13381 aggaaacata acacggattt atcctgatgg aaaagtagaa ccgtttatca atacaggagg
    13441 tcgtccactc ggtatagaat ttgatgcaaa tggaaactta attgtttgtg atgcatggaa
    13501 ggggcttctt tcagtttcta aagaaggaaa ggtaactact ctttccgatt ctgcggataa
    13561 cgttccattt cgattcacag atgatttgga tatagcaagt gatgggatga tctatttcac
    13621 agatgcgagc agtaagtttc accaaccaga atatctatat gatctgttag aaggaaaacc
    13681 tcacggtaga ttcttaaaat acaatcctta tacaaaagaa acaactgttt tgctcaaaaa
    13741 cctatatttt gccaatgggg tagcactgtc taaaaacgaa gactttgttt taatcaacga
    13801 aacctatcga taccggataa caaggtttta cctcaaggga gaaaaaaagg gaacaagcga
    13861 tatattcttg gagaacactc cgggcttccc tgataacatt acttccaatc ggaaaggctc
    13921 cttctatctt gccctcttta ccgttcgaaa tccaatgatg gatcgaattc accctcatcc
    13981 tttttttaca aaactgatgt caaagcttcc aaaatttttc tggcctaaac caaaaccata
    14041 cggactagta attcgattgg atgagagcgg aaatatttta gaaagctatc aggatccttc
    14101 gggaaaacat ttaaaggaaa tcacttcagc cttagaatac aatggatttc tttacttggg
    14161 aagtcttcac aatgatcgca ttggaaaact agaattatca aaataaaatc tttcattcgt
    14221 aaaaattgaa aaatgtgttt atttttaaac aagcagagcg gcaaatagag agtaaccaga
    14281 gaggaaaatg tatgagagct agtataattc gtaaatatgg aaacaatgat gtaatagaat
    14341 atgtggaaga ttttccaaag cctgaaattg caaccgatga agttttaatt gaaatgcatg
    14401 cagcaagtgt aaacccaatc gattggcatg ttatgaaagg agatgtaaaa cttttattca
    14461 actataaaat gccacatata cttggaaacg atggggcagg aacagtggtt caaataggat
    14521 ctaatgtgag taaatttaaa ataggggatg aagtttactt tcgacccgga aagaaaaaaa
    14581 ttacaggaac ctttgctgaa ttttgcagtg taaaagagga cgaggttgct ctaaaaccaa
    14641 agaatctaag tttcgaagaa gcggctggaa ttccactggt agggcttact gcttggcagg
    14701 ctttatttga tctaggaaat ttacaaaaag gacaaaaggt tctcattcat gctggctctg
    14761 ggggagttgg aagtattgcc attcaacttg caaaaacgat aggcgcagaa gttgcaacca
    14821 caactagcac ttccaatctt gaacttgtaa aaagctttgg tgctgataca atcattgatt
    14881 ataaaaaaga gaatttcaaa gataaattaa aagactatga ccttgtattt gatacgttag
    14941 gaggaaagac tagagaagat tcctatccag ttctaaaacc aaatgggatt ttagtttcga
    15001 ttgcgggaat tccaactcct gaaactgcgg atcattacgg attaaacttt gtaataaaga
    15061 ctttgtttta tttgcttaat tttaaaaata gttccattgc gaaaaaacac aatgtgatat
    15121 ataaatattt tctcatgaat gcaagtggag aacaattaag taaaattaca aaactaatag
    15181 aagaaaaaaa agtaaagcct ttagtagata aggttttttc tttgagagaa gtaaaacaag
    15241 cttttgaata ccagcaaaca ggtagagtaa aaggaaaagt gattataaaa ataaaataag
    15301 aatcctcctt tagacacgag gcgctgagat gaaaattaat aattaaatca tcggtgttta
    15361 tccgtgttca tcggtggtaa tcttttttga aaacgaggat aatatgaatt ttgacattcc
    15421 aaaagaagta gaagagctaa gactcaagac aagagaattt gtagaaacag ttgttatgcc
    15481 acatgaagat gactatgact ataatattgg tagacttcca gaaaatgttg cggaagatct
    15541 tagaaaaaaa gtaaaatcag ctggactttg gactccgcat cttcctaaat cagaaggtgg
    15601 actcggactc gatccagtcg gcactgcaat tatattcagt gaattaggta gatctcctat
    15661 cgctcctttt gtattcaatt gtgatgcgcc agatgaagga aatatgcatt tgctgcatct
    15721 agctgcaaac gaagaacaaa aagaaaaatt ttactaccca cttatcaagg gagaaattcg
    15781 ttctgggttt gctatgacag aaccagcacc aggagccggt gcggatcctc agtctctcat
    15841 gactaacgct gaaaaagtcg gagataaata tattctaaat ggtcgcaaat ggttttgcac
    15901 aggagcgaat ggagcaaaat ttttaatcgt catgtcgaaa gtaagtggaa gttttagaag
    15961 atctactttg tttctagttc caaccgatgc gcccggctac acaatggtaa gagaagtagc
    16021 tacgatgggc tctcatggac aaggcggaca ctgtgaatta aactttgaaa acgtagaagt
    16081 tccacagtct atgattttag gaagagtcgg agaaggtttt aagtggactc aggaaagact
    16141 aggtcccgca cgactaacac actgtatgcg ctggattggt cttgcgagaa gatcgatgga
    16201 aatggctaga gaatttgcga agaagagaga agtatttgga gcaaagcttg cagatcatga
    16261 aggaattcaa tggatgtttg cagaatctgc acttgagatc gaatctggat ttttacttac
    16321 attaaaagca gcagatacac tcaggaaagg agacgatacg cgccagatta tctctatggc
    16381 aaaatggcac gtcagtgaaa ttctatgtag atgtatagat cgcgcaattc agatttgtgg
    16441 ctcttatgga tatagtagag actctaaact agaactcttt tatcgagacg caagagcggc
    16501 aagacttgcc gatggaccaa gtgaagtgca taaaatggtc attgctcgaa atcttatgag
    16561 tggtaaattt gaattctaaa ttttaaaaaa tggaaattag tctagggaat cattattttc
    16621 taggaataat tctattttta agaagtaaaa atggcaggat aaaagatatc agaatttgat
    16681 tcgataaaat cctgcttcct tctttctttt tcggatgcaa tgatttaggg atttttattt
    16741 attgaacaat ttgaggataa aaatgaacca aatctataga ttgattctta tattgtttac
    16801 aattttccca atcgttctag caagcaaaga agagaaagat aaatgcgata catatataga
    16861 aaaagggtat caagaaagta aagttcttac taaaaaacct ataatcaaaa ttaactttag
    16921 cctaaaagat tttttcccca aagaagcaaa agaaaagaat atcaaaacag gaaagtcagt
    16981 tgttcaaatt tttttaaaca aaaaaggaaa gctggtctgc acttccatta ggcaaaaaag
    17041 tgaaggatat ggatttgacc aagcagccct ccaaatcctt cgaaaagcca aatttcgtcc
    17101 aggagaagta aaaggaaaac cggtcgactc ctacttgacc ctaccgattg aatttagctc
    17161 agagtaattt cgacgaaatt atccttgaaa attccagctc atcaacaggg ttgttgaaaa
    17221 attcattgtt aatgaatcat gtcacttcga gtgtaatcga gaagtgctaa taaaataaag
    17281 atttttcaca aagtagatct cgactatcgc tcgaactgac atttattttt tcatcatttc
    17341 taacaaaatt tcaaggataa acctactctg ttttctgaac taaaatcttt tcagggaaat
    17401 ctacaacacg ataggtgtca tcttccaagc gagtaatttt aatttgaact ggttctactt
    17461 ttgcaaaaac tccttttaaa ctatcgtcat ttgccacacc tgggagacga ccaaaaagtg
    17521 taatcgaatc attttcaatt tttatattcg cctcttgcca catgagaaaa gaaattccct
    17581 gtttttgcaa agaaggaaat agatcagaat gtagtgtatt tgcctttttt aaacgatttg
    17641 taaaatctcc atcttttaaa agaagatatc tatatttttc tcttcctaac atatttcttt
    17701 catagtttgc gagaatatga ataaattctt ttgcggtttc actcggattc tttttttgta
    17761 aaacttcttt cgcttctgtg taattcatct caggtagcat aaaacgaatc actccataat
    17821 aagaaataaa gagcccaact aaaacataag gaaaaagtcc actgagtcta ggaaatacaa
    17881 aaattcctaa ggcaatgagc actaacgcat ttgcaaacca atccaaagtc gatgtcggtg
    17941 tattcatatt cagataagaa atacaaaata caataaatag agaatagtaa acgatcggca
    18001 tctcaagtgg attttttgcg agaagaaaaa taaataaaac taaaacagat aaatcaagta
    18061 atagatggga ccaaatttca tagatggtat aatctctcac taaaaacttc cagccattca
    18121 caaaaagagg tggagcaaat aaaaatacta tcattacgat cgtgccgaaa ctgatttgtt
    18181 tttgtgtttt tgtcataaac aaaagtctga aagcctaagt aaaaaatgca agagaattcc
    18241 tttttcccaa tttatccgtt agaaggttta aaacttttat acgatgcaaa aattgatttt
    18301 taaaattcta aacagattga actagataac tttatgatct accaaaggaa taaagaattt
    18361 tagctttgaa ctattttttc tggaatctta aaaaagaaat catttgatta aataattgta
    18421 atgaaaatgt aacacaatta taggaatctg ttttatatct cgaatgtgag gaacagagtg
    18481 aatattcatg acaatggttt aaaagttcta gtcgtagatg atgaagaaga tataagtgat
    18541 ttaatccgat tcaatttaga agaagaggga ttccaagtat ctgtttgcca aaatggtcta
    18601 gaggttttac caaaggtaga caaagattta ccagacctaa taatattaga tataatgctt
    18661 ccaggtattg gtggaatcga cctttgtaaa aggatcaagg aaaaatttac tgtccccatt
    18721 attatggtta ctgccaagac aggagaaacc gatgctgtcc tcggactaga acttggagca
    18781 gatgattatg ttcgaaaacc atttagcccc agagaactta tggcaagagt tcgatccgtt
    18841 cttcgtcgtt atgaagataa aacagaagaa gataaagcgg gcaatatcac cattggcaaa
    18901 attcatctga acaaaaaagc acacaaagcc tttgtggacg gaaaagaaat tgatctcact
    18961 ttaattgaat ataaaatcct actcctcttt atgtcaaacc ctggtgttgc actcacgaga
    19021 gacaaacttt tagataaaat ttggggacat gatatttatg taaccgatcg ggcagtggat
    19081 gtaaacatca aacgattaag agataaactt ttaagcgaaa aggaaagatt agagaccgtc
    19141 cgtggagtcg gatatagatt cagtgatgcg tagtcttttc tcacgagtcc ttcttagtaa
    19201 ctggcttttc cttgtaatcc tgcttggaac agctctaatc ttactctacc tagaaacgtt
    19261 agtcgctcca gagttcaaat taatactact tgtattatac attatctatt ctatgctaat
    19321 cgcctttcat tcttcttata aaatcgcaaa cagtgtgaca gagcaactaa gcgtgattca
    19381 aaaaaagaca atggatataa atgcaggaga ttttggaact ctgctcgctg catccgatat
    19441 acgcgaacta ggtgatttag tttcctctat caattccatg tccaataggc ttcaaatgca
    19501 atttacagat ctcaacctag aaaaagaaaa attcaactcc ctcctccaga atttaagaga
    19561 aggagtcttt gctattagtc ctgataaaaa gattttattt cagaatcaaa gcattcccaa
    19621 aagcttaatt ccaacaaatt ctcagtttag aagtatccaa gatgtaattc aaaataaaag
    19681 actactagaa tttctaattg aacatattga ttcaacgcaa gatggaaaaa ttggaatcga
    19741 agataataag cattattaca aagtttggtt ctattctctt aaatcaaaca atcaaatact
    19801 gatgtatata ggcgtaattt cagataaaac agatgaaaga gaaactcaga tgctaaggga
    19861 acaatttgtg caaaatgctt cgcatgaatt aaaaactccc atcacttcta tcaaaggata
    19921 tgcagaaact cttcaaagca aactgcatgt atcacaagac agtgttgaga agagattttt
    19981 agatgcaatc ctaagaaata cagatcgaat gatccgaatt gtagaagata tgcttactat
    20041 atctaagttg gagtctcaaa attatttttt ccaaccagaa aaagtaaatc tatctgatat
    20101 tgtgcaaaat ttaaaattta cagtagaagg gtttatcaga cttaaaaatc aaaccttcac
    20161 tgcagaagtt ccggaaaaca tctacctcta tgcagatatg gttctaattg agcatttact
    20221 tttaaactta attcaaaatg catctaccta ttcaggagat aatcaaccaa tcaaattaat
    20281 tgccaataca aatgatgcat ttgttgtaat caaagtcatc gacaaaggaa ttgggataaa
    20341 agaagatcat attgaaagaa tctttgaaag attctataga gtggatgagg atcgatcacg
    20401 caaaggggga ggaacaggcc ttggattatc cattgtaaag catatcgcga agcttcattc
    20461 aggttgggtt agtgtagctt cgaagccagg agaaggaact acattcacag ttaatttacc
    20521 gattgacaga aagaagccta tgtcttaaat ttcagatata ggaagttcat gtacccactc
    20581 ggcttttatt cattcggaaa aaatataggc atcaaagatt caactcttga cttctctgtc
    20641 atctattcgg aaaaaatttg tgatgctact gcagtattca cacaaaataa ttttccggga
    20701 tctccagtca ttgtaggacg ggagcatatt caagatggga aattacaagc aatcgtaatc
    20761 aattctaaaa attccaatgt agcaacaggt caaaaaggaa tagaagatgc aagaaataca
    20821 tgtaagacac ttgcggctaa tctaggtatc cgctcggaag atgtattgcc ttcttccact
    20881 ggagtcattg gtgttccact tccaatcgaa aaaattctaa cagcttgtca gactgcaaaa
    20941 gaaaacttaa aaccaggaaa tctagaacaa gtagcagaag caattatgac tactgacaaa
    21001 aaaagaaaaa tatcttttaa agtcatcgaa tcaaatggag tcaaaggcgt tatctatggt
    21061 atggcaaaag gagctgggat gattgagcca aatatggcaa ccatgctttc ttatatttta
    21121 actgatctga aaccaactag ttctaatctt tactcatttt taaaatcatc tgttgataaa
    21181 agtttcaatt gtatgacaat tgattcggat acttctacaa gtgacacagt tgtattaatg
    21241 agtaatggtc ttgcaggaga aattcctgat tcactctttc aacaagcgtt agatgaaata
    21301 tgtattgatc taacaaaaca aattgcaatg gatggagaag gagcaactaa gctgattgag
    21361 ctggatattt tagaagccaa agatgatcaa caagctagaa agattgggaa atccatttta
    21421 aactcacctc taattaaaac tgctatctat ggcggtgatc ctaattgggg aagatttgta
    21481 atggctatcg gcaaagtatt cgatgaacca atctcatttg aaaagctatc catattaatt
    21541 ggaggaattt ctgttaaagg ggcaaactca gagacattaa aaaaattatc caactattta
    21601 aaaaataatt caattatcga aatatccgta tatctaggtg caggaagttc ttccaaacga
    21661 ttctggggat gtgacctaac ggaaggatat gttcaagaaa acgcttatta taccacttaa
    21721 tgaaagaaaa attttcgttt ttatattctt cagactatcg gtttaagggc tatgtaatta
    21781 caaaccttgt gcggttaata tcaattatcc tcacaatatc aatctatttt tctcttcacc
    21841 aactgattcc taagagtctt agttattctc tacaaattat gatgatagga acaatagcca
    21901 tatcttttat aatcattttc ccaatccaag attacttgga aaaaattata cgccaaaaat
    21961 ttgtttccca atatttggat gaggatttat cagcaggtag agtagtcaat gttgaattga
    22021 atgaactcat tagtaatctc ttccctgata tggtaaaaat ttcagaaagt aaagaaggac
    22081 gtatggtttt actcatacgt gaacctgctg attatgaaat gtatgtttat ttaaatggaa
    22141 tgagaagaaa agtaaaatca aagagcttcc aaattaaaga cagactttta gaatttttaa
    22201 gtgataaacc tgaggggata acagctgcta acctcctagg tcacaatgaa ataaatgaag
    22261 actttatcag tttaaaagta agctatgtaa taccttttat ctatagacaa agaatttttg
    22321 gttttcttgg tgtaagcaat ataccaagtg aaaaaagtat ttccaatttg tatatacttg
    22381 cgagcaaagc agcaattgct gtttataatc atatcctctc ttcccaaatt gtattacata
    22441 aaaaatacaa aagagaattt gaaatagcaa atagaatcga aggtctaatc ttctctgcta
    22501 aagttccaat cattaaatca tttaaattcg aagcctatca aaaagatcct aacgtcttag
    22561 ttgaattttt cagaaatgat gatggagaat ggctttttat tttactcgct ttaaataaag
    22621 ctagaattgg gacaggactt gtgagcagtc acttactcgg taaaatgtat tcacaaagtc
    22681 tacttagacg caaacatagt ctaaactcca ttaaatctat cacagaagat atgttaagaa
    22741 aactctcctg ggaagatgga tatgaaatgg ttgtaggtag tttaagagaa gatctatcaa
    22801 aactaacctt ctctcagaaa ggtgttaatt ttagaattac aaatgatgtg gatacaagta
    22861 aaagtttgat ttccgtagga tggcgttata cggtcgacct aaaggaaaat cctgtcatat
    22921 ttatctatta taaatcaagt aaaattattt cggtgcaaaa tatttagagt atgaaaaatt
    22981 cattaatcat aacaattgca tcgatttgtt ttattacctt taacttattt attttaagag
    23041 gaattggtct cggatcgaat tatcccaaac gatttctata cttttatcca aatggatata
    23101 ttgcaaatac tgattttgat aatactcata tgctaggaat taatattgaa gaagagagaa
    23161 ttcctaaaat tagattagaa aaaaacaaag aatccgattt tacttcagag ttttttttac
    23221 agtataagac tttaacggac ttttctcctc atattatact ttcattaata ttacttatca
    23281 catcattttg gtttttaatc aagtattcag atgtttatat ctctttgctt ttttttgatt
    23341 tatccatttt cgtttatagt aattttacag ttctggcttt cgactcgtat tactttttat
    23401 tttatttaag ttttgtgttt atggtttttt taaccattca tttgggattt agattaaaag
    23461 gaaaagaggt ttcaatccgt tggatcattc ccgaaatatg cgcatcagcc attgtcgcat
    23521 ttactgccat ctctcaaaaa gagaactctc agttttttaa tgagtttgta aaattgtgcg
    23581 gaaatttagt ctgtattggt gctgctggtt gtttgactgt attaatctat gacacaatta
    23641 agtataaaat taccggcaat cctttgatta agaaaattgc ggtaatcatt tccattttgc
    23701 aaatggtaat tattccttat agtgtatttg aattcaacct atttagagta tttaataatt
    23761 tacactattc tctctatata actttcctaa gcttccctct tctatttgta tatggcacct
    23821 atcgttatac catgattaga gaacaagtat actttgtatc ctctgttgtg attttcactc
    23881 ttttgtctag tttaattgtt ttttatattc tattaatggg agcagtaaat tatttacttg
    23941 aaacttttgc gattcaaaac ctagaagcaa tcaatgttct atttcttatt atcagcacct
    24001 actctcttgt attagcaaaa aataaaatta gagattttac agaatactgg catttcaaga
    24061 gaaatgaaaa actcacccaa actttagaac agatgattga tttagttcgc tctcaacaac
    24121 caatgcgaat cacaactaca aaattagtaa aagcaatcac agaaacctta gacattcaaa
    24181 aagttgttat tttaattcca tctgatttaa ttccagatga aataaaaaat gaaaatattt
    24241 taagaattca tagtaaatca gaagtttgga gttttttctc aaaagaaaag gatattactg
    24301 tgaccacttc ggttatgtat ggatctggaa ttcgaaataa tgtatatacc tttctaagac
    24361 aattaaatat acaattagca tttccaatgt taggtcgtga cgagaaagaa tctgtggttg
    24421 gactcatttt gataggacaa aaaaatattc ctggaaattt cactctagga gaactaacgt
    24481 tcataaagga atgtgcgaag ctagcatatt tacttttaaa taactataaa cttttactgg
    24541 ctgatattga gaaaaagaaa atggagagaa aactaaaaga agcaatgatt ttagaagaaa
    24601 caattcatcc accaatgagt agtaaaatca ataatcgcca gatggaaata aaatttttct
    24661 ctgatccggc agtgagtatt tcaggagact atgtcgattt tattcccatc aatgagaaac
    24721 aggtttttat catgttaggt gatgtttctg gacatggttt aggttctggc tacttagtca
    24781 gtgcaattaa agcaatcgtc cacgatcaaa ttgaatccgg tgtcgactta ggtaaattat
    24841 ttaaaaatat taataatttc ttaatcgaaa gatattctgg aacagaattt atgactctta
    24901 ttggaggtct ttatgactct ggatcaggaa gattcgaatt tataaatgct ggacatttat
    24961 ctcctctcat tcttcgagca acaggagaaa ttgaagcaaa gaaaagtggt catagaattt
    25021 taggtgtaac ggttggtcct tttaccactg actctattca actaaatgta aatgacaaat
    25081 taataatcta ttctgatgga gtaacagaaa ccttcaaccc attagatgaa atatatggag
    25141 aagaaagact cagaaggttt ttagcagcaa acaaagatat gccgtctgaa aacttattag
    25201 agtttctgaa gagagaatta gaaaatttta gaaaagattt ggaaatctca gacgatactt
    25261 ctttcataat tctaaaaagg atgtaatatg aatacaatta aagacaagat aattaaagtt
    25321 gaacaggaca ttgattcaaa gaagattgtc tccgttcaaa gtgttgcttt taaatctata
    25381 aatgcaaatg aactgaaaga aaaaaaacat ttaattgatt gtgaccttga ttctattctt
    25441 gtgagtggaa attataaatt aatccacaca aaaaaaatta attctgattc tgatatttct
    25501 ttttcaggtc ttcttcccgt ggtaacacta gacacgatgg gaaaagtttt aatgcaagcc
    25561 tttgtaaatg aagaagcatt gtatcaaagt atttcttctg gatatgcaac ctactttagt
    25621 cgttctagaa ataaagtatg gttaaaaggg gaaacgagtg ggaataaaca agaaatgata
    25681 aacattgaat ttgctgattc tttctttatt tatattgtaa gacaagaact tgcttcttgc
    25741 cacgaaggat tttattcctg ctattataga caactactcg cagatggaag ccttgctcaa
    25801 attgattttg atagaaaatt cctaccagaa aaagtttatg gttaattgag ggaaaaatta
    25861 ctgtgttatc aaaagaaatg ttaataaaaa gaatattacc tgcaatccta ggcatacttt
    25921 ttttaggaat cggtggaatg tttttaattg attcaatcaa aggcggtgca cccggagatg
    25981 gtagttcaaa atatgaattg attgtctctg cgggagaatc ttcaaccaag attacaaagg
    26041 atttggctag tcatggaatg attcaatcta gttcttattt tgcctatctc ctaaaaattt
    26101 ctggcaatac aaataaaatt aaacaaggtg tttattcaat tgataatgga atgactgcaa
    26161 gaaagattat ggatattctt gtagatggta aagtaaaaat gatttctttt accattccag
    26221 aaggttataa caaccgtcaa atcggtgacg tcctaactca aaaaaacata gcaaagtcta
    26281 gagatgaatt tttagctcta gcgtctaacg aagaaatttt aaagaaatac aaaataccag
    26341 caaaaagcac agaaggttat ttattcccag aaacgtatac agtgccttac aattatcccc
    26401 ttgataaaac agttgagatg atgttaaaaa gatttttttt aaatcttcaa aaaattgata
    26461 aggcgaaaga tgtttcacca gaagatctac acaagaaagt aattttagca tcgatagtag
    26521 aaagagaagc taagaaaaaa gaagaacgtc ctttgatggc tggagttttt attaaaagac
    26581 ttaaaaaggg aattgcatta gaaagttgtg caactgtgca atacttgttt gaaaagccaa
    26641 agaaaagatt atttgaaaaa gatttactta caccttctga ttataatacc tacttacata
    26701 agggttatcc gccaggtcct atttcgaatc cgggaatgcc tgcactctta gctgcattca
    26761 atccagagga aacagacaat ttatttttct tgctgaaacc cgatggatat cattattttt
    26821 caaaagatca caaagaacac gcgcaagcaa agaagaaata catagacgtt ttatatgagt
    26881 agtataaatt ttgacaacct agataaagag atctttggaa tcaataaaga attacaagat
    26941 gtccaaaaag ctctagaaga agtaaaaaaa agacaagatt ctaataaatc tgccaagaaa
    27001 gaagcgttag tatttataga gaaggctgaa gctgtaattg aaaaagcaga gaaaggcaag
    27061 attaaactaa gcgaagatca agtagagcga atcaaagaaa ctctaaaaaa aattctactc
    27121 atttttaaaa gctagaaatt ctctataaaa tctttaatat tttctacaaa ttctaatggt
    27181 gcttttttag gacgagaggt tgagagagac acaaataatt gttcatctct catcgaggca
    27241 ataatttctc cggtttcgga aataatatcc tgccttaaat aaaaacgaat tttttcgact
    27301 gattcaatcc aagtaactat ctttactttc tctcctccat tcggcatgcg aataaaacga
    27361 agtttaccac ccataaaaaa ggaagttgta tctatttttg caatttttga ggtatcggct
    27421 actttggaga aaaaaagcca acgcccctct tcaaatgctt tccatacaac atcaggagag
    27481 tatcgccaaa aacaatttag atcagaatac agaaaagtat aatcatggat caactgttta
    27541 caagttcctt taaaagcatg tatagtcggt atctcaaagc gatcagaact acttgttaaa
    27601 acaaaggttt caccagatgc cttttccaat cgactaatca atcttagttc acaagcaaga
    27661 gaattgtcct ctccgtatat tttatgattc cattccaatt gattctcgcg ataggcgaca
    27721 agttgagtct caatgcgcaa atttgcatct gaaaattgtt gagataaaaa tctaacctga
    27781 gtgaactctg gaatcaataa aattccctct tccatacatt ttcgaattgg aaaacctaaa
    27841 ctatctagaa tttgacttct accctcttga ctaaatcttt cgtaggttct acttgttaca
    27901 tggcgattcc aatctaaatc aaatatattt gttctgagag taatttcatt tttcattgta
    27961 tacattttcc gattttttat ctgattcaac catgctctta ttacttccta ttctgaatat
    28021 ccttttgttt ttgattttga atgagtaggg ttcaacaaac caatgagctt gagtaaataa
    28081 ttttcttttt tctttacata aacataaatt catttttctt tggaagtatg gatactacac
    28141 ttggcataaa tataaatact ggaaaaagtt ataccgttct tatagcagat gattcattct
    28201 cagatagaca attgattaag cgcttcttac aagcggatca attcgaagtt ttatcggatg
    28261 ttcctagcgg ggaaggagtg attgactatt taaaatattc aaaaactgtt ccagatatta
    28321 ttttcttaga ttataaaatg gaacctaaaa atgcattgga aacagtgaaa gaaattcgtc
    28381 ctacttatcc ggatcttatg attgtaattg ttacttccaa tgtaaacaaa gaggcaattg
    28441 ccgaactagc tcgttataaa atcactgcct acatattaaa accaatcagt aaaagtatca
    28501 tcgaccagaa actaatccaa attctaggaa gaaaagactt agttgtagaa tctaaactcg
    28561 tcaaaaagca tgctttaaaa ctagaagaca tcagcatacc acctcttcct ggaattgctc
    28621 aaaaggtatt actatttgaa ggtgatccag tcagcggaag cagtgaactc gaacagatta
    28681 tcttaccaga taaatccatt tgcgcagata tacttcgaat cgcaaattct gccttctatg
    28741 gtagatctaa aaaagtgcaa actataaaag atgcaatcac actactcggg ttgaaaacaa
    28801 ttaaaaatat agttatgctt caatctaaga ggcatatggc aaaaaattta gtttatacag
    28861 acatatttaa aaaatatcta ttagaacttc ctatattaaa tgcgttagta gctctagatc
    28921 tttctgttcc tatgggtcta aaaagccaaa gagaagaatt atttttatgt gccatgatga
    28981 taaaaatcgg aatgacgatt ctagctctaa actctgctaa agtttacaac gatatgctta
    29041 ctcaatatga taaagtaggt ggtgatttag taaaactaga aaaggaggaa tttgaaatca
    29101 gtcatgtgga tgttggtgtc catattttta aaatatggca aatgccagca gtttttcaaa
    29161 aaacaatgaa agaccaagga tttctcaccg ttgaatttaa taaggtagat gatttcgaca
    29221 gagttcttag actatcagag attatttcca aacgtttaac gcatattcca gttccagact
    29281 cagatataga gttagcaaag catattctca ggcattataa ttcaccagaa gaaacacttg
    29341 atgtatttga tgatgaatac tatgaaaata tcaaatctca tccatttttt agttcaatgt
    29401 aatattcaaa atcactggaa actagataca gattcgaatt ttattttttt atttttaaat
    29461 ctcaggatga tttgatttta ctaaaagcaa taactcgcct gtagaaaaca aatacttagt
    29521 aaatagatta atctatttac agtaagtagt gagaatacga ttcttgccaa aggagtcatc
    29581 tatggcaaaa gtatcgattt atttaaattt tcagggaaat acaaaggaag catttgattt
    29641 ttataagtcc attttcggga cagattttga aacaatcgtg tatcataagg acacagcgga
    29701 aaatggtcgc cctaatctgc ctgagaatga gtggaataaa atcatgcata ttgatttacc
    29761 aattcttgga ggaatacatc taatgggttc cgatatgctc gaatcaatgg gtcatagcct
    29821 taagattggg aataatacaa ccatcaatct acaaggagat acgttagccg aaacaaaaag
    29881 actattcgag tctttatcaa atggagcatc tgaaattgcc ccgtttggtg aaacgccctg
    29941 ggggcattgg ggttgcctac tagataaatt cggcataagg tggatgttta tatttccttt
    30001 ataaaaagaa actagcaaga cccaaaaagc aaaagaatcc aaatgtatcc gtgcaggcag
    30061 taacaaagat tgaagaggcg atcgccggat caattttaaa aaattttaaa atcagtggaa
    30121 ccacagatcc aatgagtccg gcaactatca tattaaacaa catagctgac ccaatgacac
    30181 tagccagagt aagatctctt tttacaataa aggttattgt gaatataatg ctacctaaaa
    30241 caaagccatt gaggatacca atgattagct ccttgcgaac agcaatatac caattatata
    30301 atgttaattc acccgttgca agattgcgaa caacgattgt gatagcctga gttcctgcat
    30361 ttccacccat accagcaaca atcggcatca aagaagctag aacaacaatt cgttgaatcg
    30421 tctcttcaaa caaagaaaca actgttgata caagcatagc agtcacaaga tttagtccaa
    30481 gccaaatgat tcttcttttg acagaatccc atattgaggt ttttaatttt tcatcctcac
    30541 tgagtccccc gagtcggagg atatcttcac tcgcttcttc ttcgacaaca tctaatacat
    30601 catcaaccga aatgcgacca ataatctttc cttcatcgtc tacaactgga gcagaaacca
    30661 catcatactt tctaaaaaaa cgagcaacct cttcttgatc catattgtaa tggatggtct
    30721 gaactggttt cataatttta ttgatctttg ttttatgatg agagagaagt aaatctttta
    30781 gttggacaaa tccttttact ataccctttt ggtctgttac atatatcagg taaatattct
    30841 ctgtttcctt ggcagcacgt ctaacactgc ttatcccctt acttgcagtt tcattatcct
    30901 taattactgc ataatccgaa ctcataagtc gacctgcaga attttctgca aagctcattt
    30961 ggtttcgaat gattgcagag tccttactat caagagaatt taaaatttct tctgccttct
    31021 ctggttctat ttctgcgata aaactagttg catcgtcaga ctctatgtct tctaagataa
    31081 aagcaatttc ttctagtttt aaatacccta caaattcagc ttgtgtctct tctcccaatt
    31141 caacaaagac tagtttttgt ttatctttat cacataaacg aaatacatag agagcatctt
    31201 cgatttttgc ggaagaaata atttcggcaa tatcagcagg atgagctttt tcaatatact
    31261 ctgcgagtgc tgtataattt ttactaacga tataagaatg aatcaattca acagagagat
    31321 gtctactttt ctcttgctga gagatcatct cttcatcacg ctctggttta aatgcaactt
    31381 gttctttcat gattctattc cttttctatt aatattttta aaaagaaaat tgagtcaatc
    31441 taagattttt taatgaaaaa caggtttatt gcaaagaaga aatttttggg tttgtgataa
    31501 catgcataga ttctgtgtct tgaaattcac cagcttgaat atctaaggat aggttttctg
    31561 ttgcagataa atcaaaagtc tctccacgct tcaagggttt tcttgataaa agaacatgat
    31621 gtgctaagtc atagataatt ttatagattt cgtcaaaggt tttatctgaa ttcaaaaatt
    31681 caatttccct aaatccaaat tctttcatac ctatcgtgaa accatttctt tttccattgt
    31741 tttctcttaa tccaaagtaa atccaattat taagaggcaa gtcatcctta gtcattgtat
    31801 cagcaatttt taaaaaatat tctttaggag aaacgagaca ttgattccct agatagacgc
    31861 ctatcgattt tgtattccta aggactgatg ctaaaatttg ggtgaattgc atattagcct
    31921 caactaaatc ctcaacatta gttgaaacag tgataataag atgagaaaga tgttctgcta
    31981 tttgtttttc tgcatcttgc caaatatggg acatcttagc tacggtttcc atttcttctt
    32041 ttggaatttc tgcgtcgaca cctagaatta cgatcctata tttttctgct gtaaagatta
    32101 ctattccatc attagaagtt tgcttgctta ttttcatttt atacttagtt tctaattctc
    32161 tttgaatgat ggaggaatta aataatggat catcctctaa taaaataatc ccagtaagag
    32221 tagttttata agttccgcga cttcctcggt aaatgagtat tagccccgaa aaaattaaaa
    32281 caaaaatcgc aataaataaa atgataagct taatcatact tctttcgacc agttttttcc
    32341 cacaaagatt taagtctctc ttttaaattt ttatccattc cttgattggt aggaaaataa
    32401 aattgaggcg aaaaatcact taactcttta ggaaaataat tttctaaaat aaaatgatct
    32461 tccacatcat gtggatattt atatccatcg gaagctcctt cttttttatg aagaaaagta
    32521 ggagcatttc gtaaatgatt tggtattgtt acattgactc cattttctcg aacaaaagca
    32581 agtgcactat caattgctgt ataagatgca tttgattttg gacaactcgc caaataagta
    32641 gtgacctgac caagtaggat acgaccctcc ggcattccaa ttttttcaag agctgtaagt
    32701 gcacttacag ccagaggcaa agcttgaaga gaagcattac caatatcttc agtggctaat
    32761 atgattaatc tcctcataat aaaaatggga tcttccccac cttctaacat gcaagcaaga
    32821 taataaagag atgcatccgg atcagaacct ctaacagatt tgataaaagc agaaataaaa
    32881 tcataatgat tttccttatt tcgatcataa ttaaaaactt gtgatgaaag ataagattcg
    32941 atactctctt tgtcccaagg cttctctggc tcaattgtat gaagagcttc aataattccg
    33001 agaagtctcc ttgcatctcc tccacttgca tttaaaatta tcttcttcga ttctgcgtct
    33061 aactctaatt tgccgccttc cttctgtaat gctttatcta atatttgaag caattctaat
    33121 tctgtgaaag gctttagttt aaatatttga catcgggaaa gcaaaggtcg attgattctg
    33181 aaggcaggat tttcggtagt cgctgcaact agtataattt gaccagattc tacagcctca
    33241 agcaaactat cttgttgaga agttgaaaag cgatgaatct catctaagaa taaaagaatg
    33301 gttccaatct ttttagattc tgtaattaat tcttttactt ccttgacccc aacagatatt
    33361 gcacttagat gcttgtaatt gagtttccaa tttttactta gaatctttgc aatcgttgtt
    33421 ttgcctgatc cgggtggacc atatagaatc attgaaacgg ggcgttttag tttttttaat
    33481 atttgaattg tttcagactg accaattaaa tcttcaaact gctctggtct aagtcgtgag
    33541 gctaatggct cgtatccact cgaaaataag ttaccactca agaagccaat tcctttttaa
    33601 catcttgtag acaattatat ataagttcgg aacagagaac acatccactg tgacagcaaa
    33661 ctagggagct ctcatttaat ctgccctcaa gaagagcaat gatttgaaat tttgcatttg
    33721 gaggtaagcc ataacgttcc aaatgatcta ctattactga atctctatct ggtggaaata
    33781 acgttggttg atcgctcatt actttacaaa tctcgcatgc cattcattgg tcttattgta
    33841 ataataacta tgaccactcg aaacatataa accatacgca tagaagcttg aaaactcatc
    33901 agaggtccaa aataaatatc cattatccaa ccaagatttt aacttgccct tttgataagc
    33961 atctaggagt tcttctctag tgggaagacg cattcctaat ttgtttgctt cctccatcgc
    34021 cttatcccaa ttcatctttc ctttatagat tccccattcg gaatcgtttt gattgtctgt
    34081 ttctgaaaat tgctccataa ttgcaccttg attgtaaggt gaaaattgat tctaggaaag
    34141 tcaatagaaa gataaggtag tcattgttaa ttttttacaa ttaaagcctt cagtttattc
    34201 acatcttcac tgaaaataag cggcacagga agaaatcttt ttttgggaat aatactaaat
    34261 gcctgatcaa agaaaataac ttctgcaata tcagaataag caaaatctgt gagaagattc
    34321 cctgttataa atttaaacga attattttta tgctgcacac gggctacccc aatccgttta
    34381 aaattttgga acatgttttc gctccactgg atcgatttta aatctttcga ttgaaatggg
    34441 atggttctag aataatctag gaacaaaagt ccattgagtt caatcataga agattcttct
    34501 tctataggaa tcgtctccat tttctctctc gtctcttgaa tggaatttgt tgtcttagta
    34561 tgaactgtat tgtaaaaaga agaaatagaa ttttcttctg gaatttcatc gagagtcggc
    34621 atctccattt cggactgatc caattttggt ttccactcag acgttgccat ccttctctct
    34681 tgacggatct tcttctcaat ttcagtggaa cttctgtgtt cagggtatct aaaattaaac
    34741 aatgcttttt tggatttaaa agcttttgga gaatttctga caatttgagt tttagagttg
    34801 ggttgaatgg ctttcggttt tttggagaca ctcattaatt cgggaccgag acttgcgatc
    34861 acaaaaaaaa ggagcccgat taatatcatt atggttgcaa gataaatcat gtgtatatct
    34921 tgaatatcct aacaaaatgg caaaaagtaa agtagttttt atttctttac aagaatccta
    34981 gtttaaaaaa ccctcataaa ataaagagag aaaagatgat tttagaagaa gaaccagtaa
    35041 gcatatcaca aaaaatttac agaatatttg caggactttt tatcgcagtc ctagtaacaa
    35101 tgcttatctt tactttaatt cctggcgatg cagaacaaaa cttactccga atgatcattg
    35161 gacaggatca aatgactgca ggaaaagtgg gagatgaaac aatccctatt gattatttta
    35221 aggctgcacg attagaatgt tattatatgt acaaacaaag gtttccaact gctgctgatg
    35281 atgcttctat gcttgagaac tgtgctttca atcaaatcaa atctttaaaa atagataaag
    35341 tattggcaaa tgcagcagga taccatgtat ctgatctttc tattaaagaa gacatttcga
    35401 atgaagctag aaaaattcac agtcaatctg gaacatccgc tggctactca agtgatgaag
    35461 taagaagtgt ggatgatatt tatagatcaa tcctacaatc tgttccaatg cactatagac
    35521 aagacacagc tgcttccatt gaactatatg aaagattcct ctatacagat gtaaaagaat
    35581 cagaagaaga acaaaaaatc cgatctgaac tggaaaatgt aagactttct ctttcttata
    35641 tttcattctc agatgaagaa ctgtccaagg taattggtga aatcccacaa ataacagatg
    35701 cagaaatcca aaaagaatat gatgcatctg ttgcaaataa aacagttccg atgggagaag
    35761 atggaaagcc acaaccgctg gaagttagaa aagcacttat ctttaacaaa ctccaatcag
    35821 aagcgaaaga aaagaaacta tcagaactca aaggtaagat tctatccgct aaaggggcag
    35881 aaaatgcaaa cttaggtgca cttgcactca ttgcgggagt gaaaatagca gatttaaaca
    35941 aagtatctct ttctagcata ggaccgcaag caaaagagaa taaaacagaa ctacctaagt
    36001 ttttatctgg atcagcattc ttgaaagata ttacgaacca agcatttggc aaaggaaaag
    36061 tcggtggacc ttatacggat aaagagaaaa ctttttatgt tgaatttaaa gacttgtcca
    36121 ttgaatctgg taattcagtc gggaagaatg ttgcgatgaa cgaaagtaat tccaacagaa
    36181 ctagaatgtt tttatatgaa attaaacaaa gcctaggcgg aatattccca atacaaagaa
    36241 ataaatcggt agagtaaatg gaaatcagag cggaatttgt aaatcccttt attgaagcgg
    36301 cttctcttgt ctttaaagat tctttaaagg tagaattgat tcgtggaaaa cttaaaatta
    36361 aagagagtcc agcaccttct catgaagtgg cgatcgttat tggtgttgtt ggttcctaca
    36421 aaggggaagt tgtttatagt atgaatttag atgctgccta taaaatggca aagcaacttg
    36481 ttccctcctt agatgatgaa agcataaaag atgaatacaa agacatcata ggtgagattg
    36541 gaaacatgac aactggtaat gctcttcgta tctttgcaac cagtggacaa tttatagata
    36601 tcactactcc gaatgtcatt gagacaaatg attcctctat caaattcgtg aaaacaacca
    36661 ctttatctct gaatctctat tcacgttttg ggcaattaga gatcaatata gcgattcttt
    36721 aattttctat tagttctagt ctctttttca caaaaggtaa gtagatacta tttttaaaaa
    36781 agggcttatt cgcattttct aaaaacatct gatagtattt cttggctcta tttaaatttc
    36841 gaattgtagt gctttcttca tagattctac cacttagata aattgcatcg tcaatatatt
    36901 cactcttgct atgttttaag atgatatccg acaaatgatc taaagcagga gtatgatcac
    36961 ctatcgtaaa caataaatgc gaaagtgccc aaagggattt tggaacaatt gaatttgatt
    37021 caggagcttc tgtgatgatg gtaagatgaa acggtattgc ttcttctttt agttttcgat
    37081 ctccttttgc tgaattttct aatccaacgg ctaattcata taatccctta aacttatagt
    37141 cttgatcaga aatgctttca ataatagatt ttgcttcatt tttctttctc tcttgtgcaa
    37201 gaagtctaat ttgatccatt ttagctaaat tagacttttc tccttctccg gaagaagcaa
    37261 tatttaattc tgaaaccgca gaggttttat cttctctatt tagataatat ctagatcttt
    37321 caaaaggagc tgatgattcg tctgctttct ttttatcctt ccctttttta tctgaagttt
    37381 tatttacatt tggtaaatta gtagtttgta agtttccagt tgtttctgct ttttttgctg
    37441 aaggctcttt tatttctttt gcttcttttg cttgggaagc tgttttctca gtagactttg
    37501 gacttttatc cttggctcca tattccccta aatctaattt tgattttggt aaattattta
    37561 cttctgtaga ttttggtttt gcaacggatt tagacttttc actttctagt aatgccttgc
    37621 caatatcggt tgtaggttta atgttttctt tctcaattgg tttaactggc tcaatgtttg
    37681 gttttgtatc tttagagaca ttcgaactag gtaactcagt agcgttagac acagacttag
    37741 ctggtttttc caaattaaga gtctttgggt ttgattcaat cactggtgca tttttagcag
    37801 attctgtttc agggactaca atgattttct caagtggaaa attagaatta gtaattggat
    37861 tgtttagttc tggctttgtc tccgtaactt caatctgttt aaattcaact tcactttctc
    37921 ttttatctac tggatctttg ggaaaaggaa tacgaatact ttgttttttt tcttcttcct
    37981 ttgttggtat tgctggtttg tcttttctaa tattacgatc cattggatct tcgattgtaa
    38041 tattttcagg aaatggaatt tgaatttcct tttttagatc ctgagaaaaa acgatagttg
    38101 aaaagacaat gagcgtagcc gtaaaagatt ttttatttga aaatagataa gtatttttct
    38161 tcatttattg attgccatct agaggaagca acttcccttt tgtatctttt ttttgtggat
    38221 ttccttccgt ttctaaacta cctgtttttt tagttggtaa ttccatattc ccagttgagt
    38281 caattttatc cttagagtct tttctattta aaatcttttt ctcagaatta ggatctgtat
    38341 tgactctatc aaaaatacct tccatatcgc gcgactcaga tccagagaga ggatcgtcaa
    38401 agttgtcttt taaatacagc tttctctttt tgcctttatt tgattcagaa ttttcatttc
    38461 gattgggaat tggaaattct tgggttgcag gaacatcgtt taattcagct agaggagaag
    38521 aatccccttt tctttttaag gaacgaatac ttttgtcatt ctccactaaa gaatcatctg
    38581 ctgaatagat tgagtttggc tctgtttgtc tgccataccg ctcatattgt tttggggttt
    38641 tataaagatc ttccccaatg agagtgtcat cacttggcat tcgaatattt tttcgttttc
    38701 ggttgtattc tcgctctata tcttcttgaa ataatgattt tgtgtctttt gcgagttctt
    38761 ggccttcctg tttggcacta ccaacgccaa caagataacc ccaataaatt ccatatccta
    38821 aagcaatcag taatacccct atcattaatt ttctaaaaat agaggcaata ctttcaggga
    38881 tcttagcgac taaatcatct aaaaatccca aaacatcttt tggtttaatt tttttaaaat
    38941 ccattattta atccttcgga cataaaatgg attttttcaa tccttttccg agaatagagt
    39001 tatgctattt tttctttggg ctttaaaatg agtagaggag cttctccttt taatacagat
    39061 tcctctgtaa taacaacctc gactacatct tctctagaag gaatttgaaa cattaaatcc
    39121 atcatgatat cttctacgat ggctctaagt cctctagcac ccgattctcg cttgatagcc
    39181 tgtgcagcta ctgcttgaat cgcttcatcc gtaaacttaa gattcacatt ttctaactca
    39241 aaaatcttgg agaattgctt gagaatagaa ttctttggct ctgtgaaaat ttgctttaaa
    39301 gtgttgatat cagcttcttc caaagttgca atgatcggta gtctacccac aaactcagga
    39361 atcaaaccat attttaataa gtcttcaggg ataacttgtc tgagggcatc agacttagtc
    39421 atatcattca cattcgcgtt cacatcggaa ttgaatccta tcgttttgac accaactctc
    39481 gatttgacga ttctatccaa atcaacaaag gctccaccgc aaataaagag aatgtttctt
    39541 gtatcaatag aaagataatc ctgatgagga tgctttctac caccctgagg agggacattt
    39601 gcaatggttc cctcaatgat ttttaagaga gcttgctgaa ccccttcccc acttacatct
    39661 cgtgtaatgg aggcactatc atttttcctg gaaattttat caatttcatc tatgtagata
    39721 atgccaagtt cagctttctt tatatcattg tctgcatttt gaattagttt taagatgatg
    39781 ttttctacat cttccccaac atagccagct tctgtgagtg cagtagcatc tacaattgca
    39841 aagggaactt ttagaatacg agcaagtgtt tgggctaaaa gagtttttcc gctaccagtg
    39901 ggaccgatta ataagatatt ggacttttct aactcaacat ctgttttacc aacattaaaa
    39961 tggattcttt tgtaatgatt gtagacagca actgataatg ctctctttgc atggtgctgt
    40021 ccaattacat attgatctaa aatcttttta atttcttggg gcttgggaac atccgcaata
    40081 tgagttggtc tttctagatg agctggctca tcagctataa tttctgtgca aagtgaaata
    40141 cactcatcac aaatataaac tccaggacct gcaaccaaac gtttaacagc atcctgctct
    40201 ttaccacaaa aggaacaatg taatttttct ttacccatat tagtcggttt tttggtagat
    40261 gccaaaacct cagcctgcct ttactgtctt tcgatctata atcacgttat caatgattcc
    40321 atatgctttt gcttcttcag cagtcatata gaaatctctt tcggtatctt tttcgataac
    40381 tgaaatatct tttcctgtgt gtttttgata gagcgaattt ataatacctt ttactcgtaa
    40441 aatttcattg gcttggattt cgatatcact tgcctgccct gtagctccac cggaaggctg
    40501 gtgcatcatg attctagcat gaggaagagc cgatctcttt cctttggcac cggcagcaag
    40561 caaaagagat gccatagagg aagcctgtcc aatacagagg gttcgaactt ccggcttgat
    40621 atattggatt gtatcataga cagcaagacc agaagaaata tatccacctg gagaattgat
    40681 gtagagataa atatcgcgat cagggttatc agcttctaaa aataaaagct gagctgtgag
    40741 aatatttgca tattcgtcat caatcccact tccaataaat ataacacgat ccttgagcaa
    40801 gcgagagaat acatctgaac ttctttctcc tctacttgtt tgctctgtaa tgtatggtat
    40861 aataggcata tattagttat cctttttcga gccgttgaca atttctcctg cgcggctaat
    40921 cgaaatttca ctgttccctt gtttattaga attggaaagt aaaaaatcat acactttctg
    40981 agaaatcaga ttttcgtaga tattgcgata aatcctttct ctagactcgt gtcttttttt
    41041 ctccaattca tttatattga gtttagaaaa aactgcatct aactcttcag gcgcaactgt
    41101 tatattctct tttgccgaga tttcatgaat tgccagataa tattttatct gatttaaagc
    41161 tctattttgg aagttagact taacagagtc taaatcggta tttaacattt ctgcatattt
    41221 ttccattggc atatgatcta ggttatagtc atgagtaaat tggtgataga gagcgtgcat
    41281 ctcctcttcg atgagtgagt ttggaatctt gtaggaagaa tttcgaacaa tttcacccat
    41341 aagttgctca gaataatgag catgcattct atcttccaca taggttttca ttctatcttt
    41401 gaatcgattc tttaactctt ctaagcttgg agtctcatcc cactctttag ctagattatc
    41461 atccaattct gggtagatca ctttagaaat agaatttgct gtaacagtaa aatgaaaggt
    41521 tttacctgca agctccggat tggctttttc accataagta tagtcaaaag atttcttttc
    41581 tccaactttc agtccaatga gcttttcatc taagtctttt tgatctggat tgagtcctat
    41641 gtaatattga ttatttttag tatcttgaat gatctcaccg gcttcattct ttgtttcgat
    41701 atctagatca agtaaatctc cgtcttgact tagttcttct ggttcttttg cttggtgttt
    41761 cgcaagttta agctgaatgt cctttaactg agattgaatg tcctcatccg tgattttgat
    41821 ttgatagact tcaactggaa tatttttata atttcctaag gtaacctcag gagccgtttc
    41881 gtaggttgct tttgcaacta gagtattgtg tctttcaaat ttctcaattt ctatctttgg
    41941 tgccttaaac gctttaaaag aaagacttgg atagagacca ttgatagaat ctgttaataa
    42001 aatcgtgact gcatcctcgg aaacagagtc tccaagcatt ttaataacca tatctaaagg
    42061 agcttttcct tgtctaaacc cattgatttt tactttttgg gcggctcttt ggtaggcttt
    42121 tttgaaagca tcttcaatat cttcaggtga ataggttaaa tctaattttg cagttgcgtc
    42181 gtcgttaata gttactttgt attccatgga ttgctcttta tagggagtga tttagtttag
    42241 cgggaaacgg gatttgaacc cgcgaccccc tccttggcaa ggaggtgctc taccactgag
    42301 ctattcccgc agtaatgttg gtgatatttc cataaaaata ttataagggg atttgtaaaa
    42361 taaattttag aaattaaaag aaaaggattt tataatttga aaagatttta tatccattct
    42421 tttcgatttc caatggaaaa agcattcctt gagacttata tacaaaatga taacaaagca
    42481 aatagtattt tccacaagaa cttgttttga atagaaattg atatacctca gataaagtgt
    42541 gaaagcacta agaagcgttc atactaatta caccatttgt ttaatgattt ggataaggat
    42601 aaaaattcat tcttttttcc cttcttttga aaaagaagta agatctgtct gtcctacttt
    42661 ttatgaaact ttttataatt ttgaataaag tcagattagc tggttttgca attatgctca
    42721 tcggcatttt ggggaatagt tacaattggt atcaagttct tcactcagga gtattctttg
    42781 agaaggcatg tattcttttt cctttgtttg cattccttgg tttatctatt gttatctatc
    42841 caatgtcaaa ggaagatagg atttctagat ttggaaccga tcagattccg attagcgctt
    42901 atccatttgg acaaaaagta ttagttggtt taggactctt gataggtatt cttcaatggc
    42961 tttggttaaa aggattggta tgattgccta gccaatctgt cgctaaaaag gcattcaaaa
    43021 atatttgaaa ctaattcttt gaatagaaag agtcaatgct gttcaatgta aaaataaaat
    43081 ccgaaaatta ttattgataa aacttttcca cagttctagc aacaattttc aaaaatatcc
    43141 tacaaacttg acagcattgc caaacgatat ccgctatgga tattaaactt tttaactata
    43201 acttctctgg aacaactgaa tccaaaaact tcttaatatc attcaagaca atttctctgc
    43261 tttcaggaag ctcgttcatc aactcatgat aaagtcctgg ataaatcttt aaagtttttt
    43321 tatcagatac tatgtggtta tccaactcaa tacttcctct tgcctccgca ataccatctg
    43381 cgtccccgtg aatcataaga actggaattt gaagatgctt tgctttctca taaattgcat
    43441 acgataggtg aaataaatgg tttgccattt gaaaagaaat tttcccatga ttgagtggat
    43501 cttttttgta atcctcaaca acggatttgt catggctaag taaatttaca tcaatatttg
    43561 catctacgac aaagcttggt agaaaattag caaatatctt tgcgagttct ctttttatta
    43621 atttttcgag atcaaactta acttttaagc ccggggaact aacgattaat ccatgcattc
    43681 gatcttggtt atgaacttct aaagtaaatt gcaatgcaat cactccacct aaagagtgac
    43741 ctagcaaaaa gaatttttct ttcttctctt tttctaatgc aatgtgtacc aagtcagata
    43801 gatcttcgat ataatactga aactggtcaa catggcctct aattccttca gaccttccat
    43861 gccctctaga atctaatgca tagatagaaa aatcagaatc ttttaatttt tccaatatat
    43921 ttttgtaacg atcactatgc tctccaaatc catgctggat cactaagagc cggtttgcat
    43981 tgggttttgt ccaagattga taaaaaatct ttgtattatc aactgaggaa ttgaaactac
    44041 tttgggtgtt tgaatacgtc atattttcct tctctaaact ttaatcactt tttttaaaat
    44101 tatctatagc gtatacagaa atttaataaa ctcattttaa aaacttttcc agatcaagaa
    44161 tagaattgta tggaacaaat atagccacct tggtaaaaaa cccaaattta gaacttttct
    44221 caaactttta tttgtgaatt ctctttcttt ctctaagtag taggaaattt gaaaaactag
    44281 agaatagaat cgatttctca gtgtggcaaa caattgtatg aaagagtttt gtaaaaaggc
    44341 tactctacca atttcctcac actcggatac aaggtttcag caagaagctg atgcccctcg
    44401 cctgttggat gaattccatc tttttgattc aattctttga ttccggcaac tttctctaat
    44461 aaaaatgctg ataagggtat tttttcttct ttcgcaactt tgggatagat cgcattaaac
    44521 ttttttacat atgctgggcc taagttggga aaagttaaca ttcgcaccaa aagaattttt
    44581 acatttggat ttttctctct gactttggaa ataatttttt tcaaattcga ctcgatgagc
    44641 tctgtctcaa ttcctcgcat tccatcattt gcccctaact ccaagacaaa atagtcaact
    44701 ccttttgaaa tggaccaatc tagtctcgct aatcctcccg atgtggtatc tccactaagc
    44761 cccgcattga ttacctttaa atcaaaacca tcttccttta acttaacagc gatccttgca
    44821 acaaacgact catctgggtt gagtagcccc attccagctg tcaaactatc tccaaaaaaa
    44881 atgatttttt ttcgattgtc tggtttcatt tcagctgttg gattggaatc ctgtttatta
    44941 caggaggtaa ccattgtgag aattccgatg agaagataaa ttattttttt catgcaaggc
    45001 tgacaaaacc aatttaaaat cattttgtca agatagaaaa tacctttttt tattgacgca
    45061 agtcaatcta aacaatttgg tactttcatg aataaatccg actcctactt agtagggatt
    45121 ttacctgaat ttaattttag atttttactt accgattgct ctaactcggt gcaagaggtg
    45181 attactcggc atgaaatgaa atattcttct gcaattctag ttggaaaaac tatgttagct
    45241 tccttcttcc ttgcaggatt agtaaaagaa gaaactacta tcagcattca actagaagga
    45301 gaaggtgata ttgaaagagt gatggcttat tcagatagaa tcggaagaat gcgtgggatg
    45361 gcaaaacaca atgctatcca agcagaagtt tccgatcata cgttaggcat agggaaaggg
    45421 atattccgtg ttacccgttg gggaggagtc caaaaactac accagtcaat cactaaacta
    45481 gatgattccg cttttgaaac aaatctttta aattatataa atgaatccga tcaactcatc
    45541 tcctttttat ctatctatct agatatggat acgaaaccaa ttcgagcttg tggtatgatt
    45601 ttacaggctc taccctttac tacatccaga caaatcgatg atctgatgga taaaatctca
    45661 aacatgaaca ttgaacaatc caagatcttt gcaggaagca tagatgagtc cttaaaactt
    45721 atggaaaata tgttagaaac taaagcagat gttttggaaa ctggcattcc tgaattctat
    45781 tgtggttgta gcttagataa aattaaaggc gttattgcgt ctatcggtaa agaagaggca
    45841 ttttcaatac tcgaggagag aggttcaata gaaatgattt gtgagttttg catggaaaaa
    45901 tataccttag atgccgagga agtaaaactg ctttttattt aatcctatgc aacttttata
    45961 tacgttatcc gatatagaca agcctattca attggttaga aactatttaa aagaaaagaa
    46021 aattcaacat ccagtaattc ttctaacggg cgaaatgggt gctggcaaaa caacttttac
    46081 ctcaaatttt gttaaatcct tctcaccaaa cctgaatcca aattccccaa cattcaatct
    46141 aatgaatgaa tataaaacat ctagtggaag tattttccac ttcgatcttt atcgcctaaa
    46201 atctccccta gaaattgaaa atttaggttt tgaagaaatt tggggcaaac aaggattatc
    46261 cattattgaa tggtggcaaa tagctaaaga ttacttcgac tcttcttctc ttgaaataaa
    46321 aatcgaactc gccgataaca atactcgctt attttcctta atctcaaaat cctaattttt
    46381 tatatgaatt ttctctagat tcctttctct tctttatcta gatatagctt cgccctacta
    46441 cttttatcta aattagaatt gctaagcttt tcttatgaaa aatatatctg ttttatccaa
    46501 aacggctctt tctgtattga aaaactatag tataatcaga tcgattatta gttaaaaaga
    46561 cttttgcgta taagtcaaac ttggagacct ccgaaattcc gactgtcgct aagtgggccg
    46621 aagaggcttt tacaaaatca acagaatcag ctattagtaa gtagaccaaa aaagctgaga
    46681 tcgacatacc aaagctaaca cgataaagtt ttaattctgc ccaatcttgt ggcatgggac
    46741 ggaaggctat tttttgtaga ttttgccctt tctcctggta tttagtagta acatttgaat
    46801 aaccaggcac aagaccattt gtgataaaaa gttggtattt agaaagaaga aaatttacat
    46861 aagcgcgaac accgccatat acagaacatt tttctagaaa aatatcgtaa taacggtttg
    46921 gaatcagaag agtggagcta aactcagaat atatatactc acctctggga aatgaaattt
    46981 tgggattgga aaaaatttta atatcatggt tcataaaaaa gcctccaatg ttaatagaga
    47041 aaaatttcta ttcagctgtg cgggattttt aataaaaaaa agatgttttg aaaaattaat
    47101 ttagaaatcg gtttattgga ttcattcata ttctataaaa aaatgaattt aaaatatgct
    47161 taaagcaaaa cgatagtaaa aatatctaga aagtttttga gaatgtaatc ttagtttgaa
    47221 tcgactaatt cttcccttgg attattagaa tttctttctt ttagaaaatt tcgaatagat
    47281 actaatagag aagcgattaa agcaattcca aaaatataaa agttggtatt ataacgataa
    47341 tctgagttct ttataaaatc tatatgaaca gaagttggac tattgtaacc gacaacaaag
    47401 agtattgcca ttatgacttc agataataat attaaaatat tggagataga agaattacta
    47461 aaaagtaaaa ataaattgat aagcgaaata aaaataaaaa aaagatttat tttagaagag
    47521 acagcccaca cttcatctgt tccaataaag ttaatcgtat attcaaacca gggacgtgta
    47581 gcaaagataa gttggactgt aaaaaaaata attgtaactt gtttaaagaa agtaaattgt
    47641 ttccaaagag agaaaagaaa aattagaaaa tgaaagagaa ctttaaacca accgataatc
    47701 gcaattggtg taaaaaagat aaaatctctt atatatttta ccagaatcat tttacttcaa
    47761 cagtaacttc cagttctaca caggcattta gaggaagagt attcacgcca atggcaaacc
    47821 ttgcatgctt cccaggttct ccgaaaattt ctagaagtag atcgcttgcc gcatttgcta
    47881 ctatatgctg ctcgaaaaat gattcagccg aggagacaaa tgctccaatt ttcacaactt
    47941 tctggatctg atctaaatca ccaatatacc ctttaatcgc agagagagca tttaggcaag
    48001 actgcttcat tgcttctcta gcttgttcaa ttgtcacatc ttttccggtt tgtcctgtat
    48061 agagtaattt tccgtccttc ataggaagtt ggcctgatgt aaaaaccaat tttccagaac
    48121 catttgcagg aatataggag gctatagctt tagggactgg tgggagcgta agtccaagag
    48181 attttatttt ttcttctatc ggcattctgt cagattttga aaaaatactc tctttacaat
    48241 aaaaaattgt ttaatctttt ctgccatttt aggttggatt gttttatggt tgccacagat
    48301 attttagata ttgttacaaa gctttttctt gccgcttttt ttggcggagt catcggattc
    48361 gaaaggaaat ttcaacataa accagcaggt cttaggacaa atatgctaat ttgcctcggg
    48421 tcctctcttg ttatgattct atcccagcac ttgtcttata ccgtttcaga atcagacttg
    48481 ctaacaggaa gaacgatgca aaagacagtt ggagatccag cccgactagc cgcccaagtt
    48541 atcagtggaa tcggcttttt gggtgcagga gctattttac atggaagggc aaatggcaca
    48601 attaaaggtc tcacaacggc agcaacaatt tggacaaatg cagccatagg cttagcggtt
    48661 ggtgcaggat actatatttt ggctggaatc agcactctat ttgtattgtt tacactctat
    48721 gtaattcgaa tgatagaaaa caaaacaaat agtaagcatc ctagaccgag gacattgctc
    48781 attattttga aaaaagtgaa aaaaatatcc caattaaaaa aggaaattta cagaaagggt
    48841 atctttattg attctgaatc cattaaaaaa agaatggatg aagtagaata tagagcagat
    48901 ttaaatatct cccctgtcat ggaggagatt ctactcaacc atttaaacga agacgataat
    48961 atactatctt ataatataac tagcgatcaa taaatgagca tatttaaaat tacagatcat
    49021 ccaaagtttc caaattatta taaatattgt gagaacacag gaacctgttt ttatattcca
    49081 gcaaaagaaa gaagctattc caattcctat tttatggaag aatacaaggc tcaatataaa
    49141 aagacttact acgaagatga acaatctctt cgtagcctag cgaaaagaag attagcagtt
    49201 ttagagaagt tcaatcgtcc tagaaaaaca aaattacttg aagtaggttc agcggctggt
    49261 ttcttcttag atgaagcaag aaaaattggt tataatacga agggcataga gatttctgaa
    49321 tcagaagtaa actatgcaaa agacaaacta ggattagatg tcgaaaatat ttcgttccta
    49381 gattttgatg caaaaaataa atacgattgt atcgcgctct tttttgtttt agaacatttt
    49441 cctgatcaag agacagttct aaataaaatt ttttctcttt tgaatgaaag cggatactta
    49501 ttcttagccc ttccctccat ctatggtcca agtttttcga caaatccaga agaatggttt
    49561 aagacgcatc ctgaagatca cttcgctgac tactctccct tttcccttaa gaaagtgttt
    49621 caatatttta acgctaaaat tttatattgt gagccaatgt cttatcatgt aagtagagat
    49681 aaaggaatga gaggaaaatt tccttttcgt tttttttaca attcgctcgc aagactcact
    49741 tcttatgggg atacaataca aatattagca caaagaagat aactatgaga tttgatgaat
    49801 taaatataca tgaaaatatg ctacgagtct ttcaagaaat aggctacgta gaattaactc
    49861 ctatccaaga gaaatcaatt ccctatgcat tagtaggaag agatttaact ggtctcgcac
    49921 aaacgggaac tggaaaaact ttagctttcc tgattcctat tgtaaataaa atactcaatg
    49981 aaaatccaga gggtcctgtt gccttgattc ttgccccaac tcgggaatta gtaattcaaa
    50041 tttgcgaaga agcaaagcga cttttaaaat actccgacaa aaaagtagca agttttatcg
    50101 ggggaacaga ctacaaagaa caattacaag atatagagga caaaacagag atcattgtag
    50161 caacaccagg aaggctgatc gaccatataa aatctggtaa actttctctc tctcaaataa
    50221 aatttttcgt tttagatgaa gcagatagaa tgttcgatat gggatttata aaggacatac
    50281 gttatgtcat gaagaaatgt ccagatgata agcaagtaat gcttttttct gcgactcttt
    50341 cttattacgt tatccgcctt gcatcggaat atttaaaaga ccctgtagaa gtttctatcg
    50401 aggcggataa agttgtcacc gataatatag atcagaaatt aattcattta ggtagagatg
    50461 agaagcttcc ctatcttgta aatttaatat taaaagatca ggaagatggg cttggaatca
    50521 tttttacaaa tctaaaaatt atggttccag aaattgttca taagctatca aagtatggaa
    50581 tcccttctac tgggatttct tctttgttag atcaaaagaa aagagttcgt cttttaaagg
    50641 actttaaatt tggaaaatat aaatacatgg ttgctactga tgtagcttct agagggattg
    50701 atgtagataa tataaaagta gtatataatt ttgatttgcc ttctgataca gaaaattatg
    50761 tgcatagaat aggaagaaca gcccgtgcgg ggagaaaagg cagatcaatc agtttttgct
    50821 cagaaaagga ttatatagaa ttggaaaaaa tagagaaatt cttaggaaca aaattagaaa
    50881 caatctcagt agatgaggaa tatctaaatt atccggaagg ggaatttacc ccttttgttg
    50941 gagaaaaatt aaaaggtgct tcaccaaatc aaaacaagca acagcgccat ccaaatcaga
    51001 aaggaaaagg aaacaacttt cagaaaaaag gagatcgcta tagagatgat aaagacagaa
    51061 agaacaaagg gaaaaatgaa aaattcaaga aggataaata taaaccaaaa caagaaccta
    51121 tgaatccctt ggatgaagca aaattatatc tacaaaaagc tgattcaatg ctagggacag
    51181 aaaaatctaa aacgtcaact catccgtcta aaaaagagac gcatccacat tcaaaagaaa
    51241 aagttcataa gataaaatca gaaaaaccat atgatccgaa aaaacagcca tatgataaat
    51301 ccaagagaaa tctatttgat atcaatgaac aaaataataa agatgggaaa aaatcggtat
    51361 caatttggaa aaaaataaaa tcattttttg gacgatagtt ctaatatcct ttctagtaac
    51421 atttgtttct attaaagctg aaacaatcaa tgtagagaaa ggatatgttc gttttaatga
    51481 tcttagaaaa aaacttccag gtttgcaatg taagctcgat acggtcatac tagtaggaga
    51541 aattcgtttc aagaaaagca cagaatttaa attcagagtc gattccgctt attacatcca
    51601 taaaaataat atagagagtt taccaaaacc tattttatat aaaatgggag acctcctact
    51661 tcccaaagaa atggtagagg caatttttat atttttaatt gataccgagg tgtcttacaa
    51721 aattactgat gatcgactga ctctaaactt atatgaaaaa gttgagatca aaccagaaga
    51781 aataccactt agacatttaa ttattgatgc aggtcatgga ggtaaggacc ctggagctac
    51841 ggcggttaac ggtgaatatg aaaaaaatta tacattaaaa atcgcaaaga tggttcgcaa
    51901 atacattaag aaaaagtttc caaacttaaa aataaaattt acaagaaaaa aagatgtata
    51961 tgttccgtta gaagacagag caaagatcgc taacaaaaaa ctgcaaacaa gtcgcgatac
    52021 aatatttata agtttacatt gcaattcaac tcctacacca gaaaaaagtc ctcatggtta
    52081 tgaagtttat tatttagcac aaagtgcacc aatcgaaatc aatagagaat cttcaatcct
    52141 ttctaatcag ctaatcgatg ttaggcgtga tcctaaagtt caatacattc agagtagtat
    52201 gctatcatcg gtaattcaga gaagaagtat tctactcgca aaatcaatag acaataaact
    52261 aaaagaatca ctagactcaa aaatagcaag tcggggagtc aaaaaaaata attatcaagt
    52321 tctcagagga agtatgatgc cagcagtttt aatagaactc gggtttatta gccacccaca
    52381 agatgtaaaa atgctcgaag aaaaagcagt tcaattaaaa ttggctgagg gaatcgctga
    52441 aggaataaaa aattatgtta tggcaaaaaa ttaaatcaat ttacagagcc tcaattgatt
    52501 tctgtaaaaa tttactttca aaaatttttt ctgaagcagt tggcgaaacc aaaagcaata
    52561 aattccgatt tttctttatt tcctcttccg ctggtatatt gttttttatc atttttattt
    52621 tggtaggtaa aaatccattt tctttactag ttccttttta cttttatgat ctaccaagca
    52681 ttgacaaaag aaccgaaatc actcttttta tatctgatgg aaaaaataac ctactaaaat
    52741 caaagcgaaa ggttttatta gcagagaatg atttgagaag aaatttaaac attataattg
    52801 gggaactaag tcaagctccc tatgaggatg tcatagaaac aacagaagaa aatgtatttc
    52861 caaaaaaact tcctaatatt cgcaatccta tactaataag ctggtttcta gaaagcgaga
    52921 aaaaattaat catagattta gatgaagaag taattcaaaa agaaatctcc aatattcgta
    52981 ttcgcgtcga tgcaaatgac gatgacctcg atgaaaaaca aaaaaaagag gaagaagagc
    53041 ttgaaaaaaa acgttatgca gaattacaag tcgctaaaat gaaaatttta aatactactc
    53101 tgcaagcttt agaaaaaact atctttgcga attttccaag tattgaatct atagaattta
    53161 aaataaatgg tcattctaaa gaattggctg gtcttgaata taaactatcc gaaataaaaa
    53221 agcgcaatta gtttgagtaa tcttcaacag ttccctatta atctgcaatt atataaaaaa
    53281 agaattctta ttcgagcaat attaatattt acagtaaatt tagcacttct tggtctgacc
    53341 gtttggtcag caccgagtga aagtcgcctt aattatctag cgataaccgg cattgtcatc
    53401 actgcaattg gtttgttttt aaataaaaac tatgaaaggc aagtctccat cctaaaagat
    53461 aattatttag aattaaataa tcatattctt caatggtata ctggcaatgg gaaatgcact
    53521 acgatcaatc tgaaaagagt aaaaaaaatt gaaagagata aatataggag cttcgaccgg
    53581 tttttaattt ttgaaggaga aaatcctgaa ataattctaa atcttctaga accagatgaa
    53641 tttcaaaaca gaatcgaaga gtatacaaaa cttaaaactc aaatatttat tatcccatgg
    53701 cgttcctatt taataaaagg agttagtttc tttattcctg cactcatcgc attcggtttt
    53761 tataaattcc aatttttaga attaaaactt ttcttcttaa ttttgaatat gaatgcaatt
    53821 tttttcctag tccatttttc tgaaaaaaga acccgaggtg gatttgcaga agctaccgtt
    53881 agacgaagca gtataattct ttttttgtta ttagcctacc aattattact tctcttcggc
    53941 tagttttact ctattctatg aataccgatt ctttcgatat cgaaaaataa tcgtatagta
    54001 agatctgttg ttggaaagta tgacttttgt ggacaagtca aaattcgaga tcactggaat
    54061 tccgacagtc actaaatagg acgaggcggc tttttcaaat tctacaaaat ctgcaatcag
    54121 tagatagacc aaaaaagctg agatagacat accaaagctt atacgataaa gcttaagttc
    54181 cgcccaatct tctggattag gacgaaaggc aatcttatga agattttgcc ctgcctcttg
    54241 atatttcgta gtaacattag aatagccggg cacgagacca ttggtaataa aaatctgata
    54301 ttttgaaaga agataggtaa cgtattcccg agtgcctcca taggctttgc atttcaattg
    54361 gaacatttgt aaatagcatt ccggaatcag aagagttgaa ctaaattccg aatatttata
    54421 atcctctctt tgaaactcga atttttgatt tgaaaaagtt ttcccatttt tattttcctt
    54481 cataaattac acctctatga taatagggaa aaaattttaa aatctgtgcg gtacttttgg
    54541 aattttttta tgaactgtaa attttttttg aatattcgtc ggaactccta cgatggttat
    54601 ggggaattcc gacggtggcg actacgattt gcttttagta aaaagtttag gcagccatta
    54661 ctttttcggc aaagggatct ggaagagaga aggcaaaaat ccttttccaa acagaagcaa
    54721 actgactcac aaaactacca gtgttataat gctgaccata cttctcacaa agatcctgta
    54781 gacggggagc aatttcttcg taacgattcg cggggatatc ggggaacata tgatgttcaa
    54841 tttggtgaga gagatgtccg ctcatgaggt agaagagttt actgccttct aggttggagg
    54901 agccttttag ttgcctgaga taccattcag cttttgattc gttctcaatg tccttcggag
    54961 taaaggtttc tgcgttttcc gtaaagtgac cacagaagat gatagagtaa gtccataagt
    55021 tacggataac gtttgcgaaa agattaccca taaaaatttt aggagccatg aaaccgccga
    55081 gtaaagggaa aaaaacaaag tctttaaaaa gttgaagttc agctttttta ttaaaaacat
    55141 ctttgtattc ttggaaggtt cgtttcgttc tttgtttttt aggtttttct aggtattcaa
    55201 ctttatagcc atgcccacca acgccccatt cgaaagtaaa agccaagtaa acgtttgcaa
    55261 tcgtttggaa aaggtttgtg attttccaag gttgttcaga agaaagtctt gtaaagtgat
    55321 acccatagtc atggtcttta ccaataacgt tcgtataggt atgatgcata tagttatggt
    55381 aaaatttcca ctgagcagaa tcagatacaa tatcccattc gaatttgttc gagttaaatt
    55441 caggatcatt catccaatca tattgaccat gcataacgtt atgcccaatt tccatattgt
    55501 ttaaaatttt agaaatcgca agtaagctag ttccaagacc gaaagtgatc gggtctaggc
    55561 taaaatgaat caatagacga ccgatgattt cattgtagcg gtagctagct tgaatgcggc
    55621 ggatgtggtc agcgtcttct tgaccaactt ttgcaagaac ctcagttcga atggattcta
    55681 gttctttacc aaatgcttca atttcttctt ttgttaatgg tttactttta gtattcatag
    55741 tattttcctt tttcaattat agatctaatt ctaaattttc ttctgctcta ctgaggcaga
    55801 gttgaattgt ttcgtctttg ttttcagaga cagttccttc ttgtaaattc gtaacagttc
    55861 cagttttttt tgtgcaagca caagtatgac agattcccat tctacaacca ctttgcggat
    55921 aaattccgtt atcctctaat tcttccagaa gattcttttc tccctttact aaaatcgttc
    55981 tatgcgattt agtgagagta accgaaacat cttttaaatg tctcttagca gatgatttca
    56041 ggaatggttg aaagttttct gaaataattt tatcttttgg taacagatct ttcacgctgt
    56101 cttgtaaaga tttaggacca caaagatacg ttgttcgcaa atcaaaatca ggaactaaag
    56161 tctttagcat tttttccgag agaaaaccaa attggaatcc atttgattca atatcagtta
    56221 agataaaatg agttttcaaa aaggaattat tcttttctag attttgaatt gcctcatgga
    56281 aaataacgtc ttcttttgac ctagaaaaat aaagaagagt tgcttctccc gtataaccca
    56341 ttttagaaag agtctttagg atcgaataga ttggggtaat tccacttcca cctgcaatca
    56401 aaagcaattt ttctggaaga ttttccgtta aaacaaactc accctttgct tctcccagct
    56461 caatcacact gccaagactt aggtggtcat gcataaaatt agaaacaagc ccatcctttt
    56521 gtcgtttaat tgtaatttgt agcaaacctt cgcttggagc agaagaaatc gaatagtaac
    56581 gagtcagcct acgcccgttt acttctacag tcacaggaat gtattgacct gccttaaaac
    56641 tgatctttcg gttcgtttcg attgtgaaag ttctagcagt ttcagtttct aaatgtatac
    56701 ttagaatacg acctttcacc ttagaagtag agaacattgg gtttatctcc ctcaaaacaa
    56761 actcaatcca gtctttagga ttgaaatact ccttatattc ttgcatttta ttgtatttta
    56821 atggaaaagt tttcatttcg cctcgcttaa gtgagttaac acttgttcac taatatttat
    56881 atattatttc cttcgtcaag ccaaaaaaag cttttttttt cagaaagagc caaaaaagta
    56941 aaagaacaga tgaagctaaa tcaaagatac cagcaaaaac tacgcactag aaccacattg
    57001 attcaggcgg ctcttaagtt aatgggtgag gacaaaagcc tcggatcact cagcctcaga
    57061 gaaatcgcag gcgaagcagg tatcgttccc gccgcattct atcgtcattt caaagacatc
    57121 gaagaattag gtcttgccct tgtagacgat atgagcgtta agctcagagt catcctacgc
    57181 gacgcacgaa agaaaggagc atacaaaaca gctctccaag tctccattga actttttttt
    57241 aactatgtaa aaaacaatcg acttctcttt cgcttcatca cacgagagcg cgtcggtgga
    57301 agtagaagaa ttcgtgaagc cattcgaaat gaaatgtcgt ttatcgcatc agaacttgcc
    57361 tccgacatga aaagacccgg aatgcctttt tctaaattgg aattcatctc agacttcatt
    57421 gtcacaactg cttttacttt agcgggcgac tatttagatt cagatccaaa cgatcaagca
    57481 actggaaaaa aactaaaagt aaaaagcatc aaacaacttc gactcatctt tcgaggatct
    57541 ctcatcacca aacgtcgaat caaaaaaaga atcaatccag aacaggtaaa tgaaaatgac
    57601 ctcagatgat agaaaagcat ttttagaact agccaaactt tccattccct atttactttt
    57661 ttctttattc tattactctg gaattatttt cttggaagaa aaattcggag atcatattta
    57721 taaaatctca ggacaaatgg gaactgtctt tggattagca cttgccttct ttcttggatt
    57781 tagaatgaac acagcctatg atcgctggtg ggaagctaga aaaattttag gcgaattagt
    57841 caatacttct agaagctttg ccgcaaaggt tgttacttac ttttcaaaag acactaatgc
    57901 ccaaaaagtc ttaatcggca aactcttatt agcctacatt catcaactca aagaccaatt
    57961 aaaacaaaac tcaaatataa aaaactattc tcacctactc gacggaaaag aaaaagactt
    58021 atctcaaaaa tccaatccag ccgttcaatt gttaaaagag atttctatgt ctatcgaaaa
    58081 aaattggaaa accaaaacag atctagataa aaatgatcta atgcaacaca tctctcgttt
    58141 ctatgaaacc caaggcaaag cagaacgaat cctaaacact ccttttcctc gcatctacaa
    58201 gtctctgaca aacgttatgg tctatatcta tgtattgctc attccctttt ttatgggtga
    58261 tatcgatcta gggggcgaag tcagtcactg ggaatatctc tccattccta tcatgtctct
    58321 cattggttct atttttttga ctgtaaatcg aatgacaaac ctatacgcag atccatttag
    58381 caaccatgtc atctcactcc ccattgataa aatcacatcc acaattgaga aagacattat
    58441 tcaaatgttg tgaacacttc ttcaaaactt ctaatcgctt ttgtattttc aatctcactc
    58501 tttgttggaa tttattttct taaacctatg ataaatactt ttttacatcc gcaaaaaact
    58561 gtagagatgg tcattgaaaa actaaaagaa gaaagagaaa aagaattttt agaatcctat
    58621 ccagatcttt atagtttgca tagcatacgg ctaatcactg ttaaagaaaa acaaatttta
    58681 gaagtatgga gcaaggcaac tgagaaggat gaatttatct taagaaaaac atatccttat
    58741 actagcttca gcggagtttt aggtcccaag ttaaaagaag gcgataggca aataccagaa
    58801 ggcgtttatt ccatagaagg tttaaatcct aacagtaagt ttcatctatc tatcaaagtt
    58861 ggctatccaa atgatttaga taaaaaatta gctttagaag aaaatcgaga atcgttaggc
    58921 ggtgatattt tcattcatgg aaaagcatcc acgattggtt gtattccaat tggagattcc
    58981 aatatagaag aactttttta cttggtcgct actgtaaagc caattcattc ttctataatt
    59041 agtatgcctg tcgactttcg tttaacagaa aataaccgct acatcggaaa tacaaacgta
    59101 gaaaaagaaa tctacaaatc catccgacaa gcgctacacc attcatatac aaattctaaa
    59161 acgtttttga atagataata aagaattatt tataaataaa aagatttgca tagtaaggca
    59221 ttccttttca tcctactaaa ggttagtagt tttctttgca aaataacaaa cgccagttcg
    59281 atgtaacaaa aaatttaaaa gattaccaca ttatgttgcg accgacagta agcaacattg
    59341 agtttgaaca ctgatggaca cggataaaat aataataatt attaaaacca tccgcgctta
    59401 tctgtgttca tccgtgtcta tctgtggtta aatattctta tatcgaattc acattaacga
    59461 gtatccattt gttatttgaa aggaaatcat tttcaatcca acatcaccgt tctacccttt
    59521 ggaggaaaaa caatatgaaa acaaataaat tcttaatacg actgtgccaa gtcacactga
    59581 ttagtttttt aatattccat ttcatccaat gcaatgaccc aaatgcagaa gaaagaaaaa
    59641 aacgtaatga tgataataaa actgcattac tcgaattgat tggtttgtct tggggat
//
LOCUS       DAOQOS010000015        58072 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_200_length_58072_cov_6.592016, whole genome shotgun sequence.
ACCESSION   DAOQOS010000015 DAOQOS010000000
VERSION     DAOQOS010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 58072)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 58072)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58072
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_200_length_58072_cov_6.592016"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1320)
                     /locus_tag="PLF66_05075"
     CDS             complement(<1..1320)
                     /locus_tag="PLF66_05075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63445.1"
                     /translation="MEKFWKTLSSLMNKTVTGARFLTIIGWLLLWYVITTWLVLGTLS
                     SHQFQQRLTNIVDKQTKKNYSVPALLRLSNQYEKNQIKIKEIKTRIYDLLKRLEQSDR
                     KLTLLKAERTTFPSNFIDLANTLKAEFKDVKTQYANLINQTKEQKENIPPVNTPTTAN
                     INNPDKVLQILAEKHEENLKQLDKLITNLEANQKNLAQSEKEISEQLHALLQAVEELE
                     VAEAEEAKMDNDELRDFLVELRYLTKLRFDILATMPAQLLTLILTLSMGSLGSVIFLT
                     RELFREQEKHSINWYIFRPFLGMVTAIAIFVLAKSGQLVISDGGNETNRMYETLNPFF
                     ISFLAIISGVLSEQAYEKIHNTGKSFFRAGAGENARWGVHLKKIMESQNKTVAEAAKY
                     LNESIDVIQDWIDEQDQVPEEAQKIIAAWLGKPSREIFSDQSPVSTKK"
     gene            1544..2167
                     /locus_tag="PLF66_05080"
     CDS             1544..2167
                     /locus_tag="PLF66_05080"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HMZ63446.1"
                     /translation="MNKILLFTLVLGLAYCEKAPEAPKAETSKPAETAKVEGTSVGLD
                     LTKSSLKWIGTKLKGKHNGTVKISEGNIVVKDNKVVGGKFSLDMASIEDLDMKDDPKM
                     KAKLEGHLKSEDFFNVEKFPKATFEITSVTEDASGQATITGNLTIKDVTKGVTFPAKI
                     TYGADKKPVSATANFNINRQLWGISYPGQPDNLIRDEINFDLSLATL"
     gene            2650..5193
                     /locus_tag="PLF66_05085"
     CDS             2650..5193
                     /locus_tag="PLF66_05085"
                     /inference="COORDINATES: protein motif:HMM:NF019935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-PII uridylyltransferase"
                     /protein_id="HMZ63447.1"
                     /translation="MQLTYIVDAEILNLYNILEKEYPNIGERFCIIAIGGYGRMELAP
                     YSDIDILYLHNGLDESILTNIISRINTFLYDSGKEVGHSCRTIDECRDNLDNINTFYA
                     MLDSRFLAGSEKLFSDFENQFLKNLPMDLLSFHNADKINYLTGVVNSDYPLLVAEPDL
                     KNGFLGLRNIQAVYWIEKSTNYIPSLSGLALLPVFTHGEVQQIESAYDFLLRARIALH
                     LIHGRRVDRLDLTEQPTVAEYMGFGAKSELTAVDRLMNTLYTHQNEIHSFLGIYLDYK
                     KADASQMVTLGKSEFYLRQLGSDLYPPKASQLFSNPETLYGDILQIFYIAHKLNLNLS
                     PSLIGQLKFASNFLQEDFKNSNLAIEIFLKILKESKQIGKILTSMHRAGILGNLLPEF
                     GACANFSLFSYHHQYPVDEHTLFILRELDKLLNGNFEDKEVQAVFSECEHVYILALSI
                     LIHDAGKVKLGDHCQYGAELATAIGERLGLNDEETDLFKFLVEVHIHMSELTSKRDIS
                     DPLLLNDFASLIGNENRLKLLYVFTVIDTKSVGPNVLTNWKKSILYTLYKNTLEVLRN
                     RENSILSPNTSKDHELLRNYLTQKEKIEEDLSNKIVEFASSMIPHTYQRYNTPRRILH
                     HFILHTKCKSHPEEIPEIEFEKEPAYVTITVYSLEDRYLLSDLTGTVTSEALNVIGMR
                     SFRNQSGFVISQIQITDSFGGGDIREEKLDRLKRNIKSVIEKKLNVENLLKSPVEWVS
                     YNKIPDGMVAQKIEFNNVLSADYTILEILLPDSLGLLYRILKQILSFDVQLHFVRIST
                     SADYAYDSFYIKSSAGNKIEDVELLKKMEQEIANAASQKVQVDVSYIEY"
     gene            5196..6827
                     /locus_tag="PLF66_05090"
     CDS             5196..6827
                     /locus_tag="PLF66_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004785085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HMZ63448.1"
                     /translation="MWWKEAVIYQIYPRSFYDSNGDGIGDLQGIIDKLDYLNGTTNSL
                     GIDAIWLSPIYPSPMVDFGYDISDYEDIDPVFGDLKTFKKLLKEAHKRNIKIIMDLVI
                     NHTSDQHPWFIESRSSRKSKKRDWYIWHDAIDGKPPNNWMGAFGGLAWEWDEKTQQYY
                     YHGFTKEQPDLNWRNPEVKKAIFKMIAYWLDMGVDGFRLDVVNYYIKDKKLRDNPKDY
                     FKGLRPYDIQHHIYDRDRPKVHSILKSFRKLLDSYEGDRMSVGEVYIEPPGNPKLAAS
                     YYGENSDELHMAFNFAFLYCKWDPNKFKEAIYHLEKELKPDDWPNYTLSNHDQIRHIK
                     RYEKGDETIHRMKIAAMLMLTLRGTPFLYYGEEIGMEDQAIPKDEIQDPLGKMYWPIF
                     NGRDKSRLPMCWNANEYAGFSTAKPWLPINKNYKKINVEAESKKPHSLLNTYKKLIAI
                     RRSSDILKLGSIKLIDDPENKVLGYFRIYKKNKILVLLNFKNEKANLDLHNYTHPKTK
                     ATLIYSTDHKRHEGENLKLPHIHLHNYEGMIIRFE"
     gene            6864..7244
                     /gene="queD"
                     /locus_tag="PLF66_05095"
     CDS             6864..7244
                     /gene="queD"
                     /locus_tag="PLF66_05095"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010571739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HMZ63449.1"
                     /translation="MDEIELIRDFSFDAAHLLPNVPEGHKCKRLHGHTFRFKIHLKGK
                     VDPKTGWLMDFGEIKKVVKPLIENYLDHFYLNDVVGLENPTSENIAIWLWNKLKPELP
                     LLYKITLHETCNSACVYMGQKHES"
     gene            7234..7905
                     /gene="queC"
                     /locus_tag="PLF66_05100"
     CDS             7234..7905
                     /gene="queC"
                     /locus_tag="PLF66_05100"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002631248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HMZ63450.1"
                     /translation="MKAKSNKGALVLFSGGLDSTTCLFFALERHREVTAISFDYSQRH
                     KIEILKAKKITSLLKLPHRVIRIDPNIFKNTSLVNKEIKVPKNFSSNEKIPNTYVPGR
                     NILFLSYATSIAEGLGYESIYIGVNALDYSGYPDCRPNFIKAFQKAITIGTKSGNEKK
                     RIQIKTPLLHLSKKEIVIMAKKLKVPFGLTHSCYDPIKGKPCGKCDSCLLRKKGFMEA
                     GVIDK"
     gene            7902..8603
                     /locus_tag="PLF66_05105"
     CDS             7902..8603
                     /locus_tag="PLF66_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63451.1"
                     /translation="MNFLKILLFPILLFFCIDCATYWNNRRKDLQDVITIGAEKPVYG
                     FSLKLAPVHAGFLFVGGETEPGKKDLGDGAGLRGGSFSSYFSQQLVFGWLGGETFYSG
                     ELELNDEGKIQYENKIPLVKEPRDNIKSHDLKYIDIIRDPPKNRAKRKKEEVRRVIIK
                     KALEDNPNASPTVLSYLPKENPKPFGYPSSYLYQIEITIGLYGGVRFGFNPAELLDFV
                     LGFTTYDLYDDDKKE"
     gene            8611..9261
                     /locus_tag="PLF66_05110"
     CDS             8611..9261
                     /locus_tag="PLF66_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000902962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2461 domain-containing protein"
                     /protein_id="HMZ63452.1"
                     /translation="MIEKTTLDFLKNLKKNNSKEWFEKNRSHFEKAKQNFESFITQMI
                     FEMAKINPDLVGVEPKKCIFRLNRDVRFSKNKEPYKNNFGAAISENGKSMETALFYFH
                     LEPGNKSFIAAGRYMPDSKALRSIREKILEDSSAFKKIIESKKIKEIFTGLSDLKVKT
                     VPRGYSKDHPEIELLKYTSFIVEKPVSDEFILSKEFTKLWIESYKAILPLLRYFNS"
     gene            9403..10380
                     /locus_tag="PLF66_05115"
     CDS             9403..10380
                     /locus_tag="PLF66_05115"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HMZ63453.1"
                     /translation="MQSFLWSSGLLKLYPHLLHVHTPFTPLIGAFLERYLLMMWENKS
                     EPIQKFLLKCILSISPVLLTFPLFFWSTEQKLEYIETCYTLGTPLRSKIVIFFIISVL
                     LFFFLGLLKKFIAFFRSSTLYSSANLKLVLGILALGFVTIVIGGFFAALGSHVGMEMN
                     GYIIGCFLILLYILRLRNPEIFKEVQKIVEEEKKYKNSQLKSVDLNTVSEKLNHLLEI
                     EKIYREDNINLTELATQIGISNHQLSEYLNQELKISFFQLIHRYRIKEAKEKLLAQPN
                     ETILSIAYHVGYQSKSSFNDIFKKETGLTPTEFRKKFKIRPDLSSRTKK"
     gene            10456..11295
                     /locus_tag="PLF66_05120"
     CDS             10456..11295
                     /locus_tag="PLF66_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008594666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HMZ63454.1"
                     /translation="MPFTCEFSLECVLQAGSYHIFMNFVRYYPIAGLAFVIFYLWKKD
                     FFARFKIQDKDPKSEKIWNEIKQSAITLTVFATIGTTVFTLIKLGVLKSQMYKDPSLY
                     GGIPYMIFSFVLLTVWHETYFYWIHRLMHHKSIYKYVHQVHHQSVNPTPIAAYNFHYI
                     EAFFEAIYAVIFTLTVPIYYPVFIFHTFYAMIMNIWWHLGYEFLPKGFASHWFFKWIS
                     TSTHHNLHHQKFNGNYGLYFNFWDRVMGTNFPYYETYFDSLADKRELNKPQPKEAHST
                     ATA"
     gene            11419..11934
                     /locus_tag="PLF66_05125"
     CDS             11419..11934
                     /locus_tag="PLF66_05125"
                     /inference="COORDINATES: protein motif:HMM:NF019235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1564 family protein"
                     /protein_id="HMZ63455.1"
                     /translation="MKNIFSKHKFTFPRKNLTLHDFSSTLLIPEKYSDLFQKKVIQHK
                     GTRAYINYLLRKYQLFIANGMIPGYSNVTTKYQEKGQNLQKIAFRPRPEDWAELKLYR
                     VSFGMSISAFIVYLLIADSVEFVNAVSNHLTGVGISMTSNFDLIAKVCLCNKKTYYTT
                     IFQYRRKYHDY"
     gene            12163..12729
                     /locus_tag="PLF66_05130"
     CDS             12163..12729
                     /locus_tag="PLF66_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002977008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HMZ63456.1"
                     /translation="MIRILLFLLLSLFIGCASGGEMLDENKEIVSDSGGLTRQELENA
                     ADKIAKDIIVNFKKNPNPKGVALAILQTKNDTSEQIPTEVFENKLVATLLSGKITTIR
                     TDKRAEQLKEIKLSQQLGTDLSAANLKSPNYFIRTSIDENMFRSSGDKIVEQVLNVEL
                     ISIETGEVSFSNKKIYRKKAVSNRGVGW"
     gene            12731..14167
                     /locus_tag="PLF66_05135"
     CDS             12731..14167
                     /locus_tag="PLF66_05135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63457.1"
                     /translation="MRLFKYVSLLCLLLFISYCASNYVEKISKAENAFYAEDYDTAIP
                     AVRDLAKDASSKDELLYLMEAGTIFHTKGDYATSNKVFKDAETIADQIQTSISKESLA
                     FLLSDNESNFKGESFERVLIKLYMALNYMMLSDIDSAKRYFKKVNFELTDMKTTDSKY
                     KQNLFARYVDAVVSEQLTAFNDARVSYKNIMDIAPDNKEILGDRYVLAVKEKDTRDQA
                     KYAEGKNYIKAYDKNLQPIPYNPNLAEVLIINQAGKAATKESKGKLVDEPMIQQALYK
                     AVEYTMRSMDTKGASVATVMAMISNAENPIPEYKKRDEQKSNPIDIYVNGINVTNTKI
                     YNDYTDTAIGNFNENYTSLVTKNIASIATKVVAAGIAAYAAGKAAENAAKGRNRNDGG
                     GAEIIGLIIGAAAGAIAGAGVSATIKPDLRCWRTLPSNFQIRRIFLEPGTYDFEFKSN
                     TSRAMGGKNFTKISVDASKLVVINFRSL"
     gene            14258..14791
                     /locus_tag="PLF66_05140"
     CDS             14258..14791
                     /locus_tag="PLF66_05140"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HMZ63458.1"
                     /translation="MFNRLTVFSLMVCLTLGPILPQTKDDTKIELKFSIDTRFGLGKG
                     VFKKTSLMVKDKKAGTGLVEVDVASIDTGLAMRDNHLRGEDFFDVQKYPKAEFEILGI
                     EESGDTVKGKGNLTLKGITKEYSFTANIIRTADVEKYTGSIKINRKDYGMVYQSMMNP
                     IDDIANLEFTVTLTSKK"
     gene            14797..14988
                     /locus_tag="PLF66_05145"
     CDS             14797..14988
                     /locus_tag="PLF66_05145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63459.1"
                     /translation="MKAVLTLIIAIYSGILLSPKISLNKESLDLNSSNFIASNSDDEY
                     DDEEGSEEEQENEEIEENA"
     gene            14995..16551
                     /locus_tag="PLF66_05150"
     CDS             14995..16551
                     /locus_tag="PLF66_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010571054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HMZ63460.1"
                     /translation="MDDSNSFFGKIFSFIKSLSVARAVSFSFGLAIVFGSLILYLVEA
                     GNISYINALYLSASSFCVTGLTPIPISKLSFAGQFVLMIFIQAGGLGIIVSTVLVGLM
                     VIQGLSRNTKLQEFMFEVLDTDLKNEDKQDSIEQPKVLRVIIAIFNIAITIEIIGAII
                     LYFTFPENLPPNVNSRVFLSLFTSISAFNNAGFSISDDISFLINDLKSLYVIIFLIIM
                     GGIGFPVIIFIEKVMLQTLNEITSKFEVWCETHLMIKAISGEEPSPIYFFLTKLSFWT
                     ENQIASYNKSLLGESNRAQTAIILIGSLILLSLGTFSFFLIEYSNPATLGNLSLSSKF
                     ANSVLVSAASRTAGFNTFNMSGILDPSIVIICSLMFIGGGPQGTAGGIKITTFVIFLQ
                     YLRNVINSQGKVEIYGHLVSKRSVAQSIRLYFLATTALVGVVFLLSLIDSSHKRLDLI
                     VFEVISAFSTVGFTLGLTESISDIEKIIYSIVMYVGRIGIFTLLVSITGNSVTSQMGD
                     QDDGLKIQVG"
     gene            complement(16557..18695)
                     /locus_tag="PLF66_05155"
     CDS             complement(16557..18695)
                     /locus_tag="PLF66_05155"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HMZ63461.1"
                     /translation="MNNKLDLTNEQIESFGKEIEEIYQEAKSKIGHEDLDYILNVKRF
                     SDALEIVGRSLIHISQEPLLWTSGSLLLGYHFLIEFSELGHNILHGQYDDIKNNNTVL
                     SSNWKWDITMDEEDWKFEHHRVHHPFTNILGKDNDFGFLVYRVNDNQPWKLYHLFQVP
                     MLLSMPLINSFFFPWYVATSRALAESRDVLTLETYLPSIQKVSKHIVKNYIFFPMLPG
                     GNYFKIAAGNFFAKLFQNIYLEMILAISHLHKDAYVFEEKDQESKGEFYLRQILSTVN
                     FDSPPYYEIVYGAINLHIEHHLYPDLPPNRLREVAPKVKALCEKYGVPYRTGSFWEQF
                     MGVIENALLRSLPIQPEDNGNIWKLLLNPIELTNRILSGFFNIGNTFSTKQSFSISEA
                     EVIDVESEIGGDAKSIIIQTPKEWGSVEWEAGSYISISILINGKRYIRQYSLTNPDFS
                     KKQFHITVKRVVNGKVSNYINDFLKKKDKITILGKPRGDFFLKKSNYKKLFIAGGAGM
                     TPILSMLRNLEKENRLSGCILIYFNRSYEKTLFLKEIYELEKKGLKSKLYFDDYKEGF
                     LTGSISKEIFDDIEELTNKEIYLCAPKGFIEKTKTLLKELEFPESKLFYENFLPDSYV
                     FDPARENVFHTVKFLRSNKEVVVNENTSLLKAIEAVGIKIPSGCLRGMCKSCQVVKTN
                     GTTQSSLSKNQRERITTCNSHPRSDIELDI"
     gene            18737..19417
                     /locus_tag="PLF66_05160"
     CDS             18737..19417
                     /locus_tag="PLF66_05160"
                     /inference="COORDINATES: protein motif:HMM:NF024701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HMZ63462.1"
                     /translation="MSSFKVDNDYIKFIFGYMKKKKNYHHGNLKSACMEEGLRLIQKN
                     GSYDFSMRDISKNLGVSHGAPYKHFETKEALLASLAEAGFHKLTNQLEEVKNENYKNK
                     LELLYKIAVMYIQFGTTNPEYYKLMYGGIIQDAEMYPSLYEASVKCYNTFLETIESLM
                     KSKELIPSSDSNLLTFYSWSLLHGLVSLAQDNRLNIAFREANANNPQQLMEELFSYFL
                     NGVGHNLK"
     gene            complement(19424..21166)
                     /locus_tag="PLF66_05165"
     CDS             complement(19424..21166)
                     /locus_tag="PLF66_05165"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HMZ63463.1"
                     /translation="MKSYITEVIDRICSLGMRNSHTVFEKRQIKTLNQISFFLGIINA
                     TLFLFNLIAQRFIPLGLNISVIFIISVPIIWFNYIDRNKIAQIYSFFASNTLILMYAV
                     FGVFNGRNVNFEVLILGCGFFGAFIFNTGWVFISLFYNLLSYLIIAFLKVKYLSINPD
                     TFFLGSIINSFATTAFLYTIIHIYKKDFKYAETILIQSEDRLNLVLKGTNNGWWDWDF
                     ITDNLYYSPLWWNMLGYEVNEIESDSSLWLRLMHPEDRKRIEVKTQELIQSNITSYEL
                     EFRLLGKSGVYIPILSRGYIQRDDTGTPVRVSGSNMDLTETKQVQAKLESLIKSISEK
                     NEELQKQKSEIQIQSEQLAELNRIKDKLFSIIAHDIRSPLTSLLLTLNSVRDGVLSIE
                     ELSDSIPDITNNANKILELVDSLFGWAKSQMGGAKVHLTTFDISKVLEYEIALVELRA
                     KNKKINLNHNLPKSFKVYADKNMIQLVVRNLLSNAIKFCKEGDRVEVNLLEKEDNVEI
                     SVIDTGFGISSENLKKIFSIEATSTIGTSGELGTGLGLLFSKEFIEKNNGVIGVESEL
                     NRGSRFFFTVPLAK"
     gene            complement(21727..23199)
                     /locus_tag="PLF66_05170"
     CDS             complement(21727..23199)
                     /locus_tag="PLF66_05170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63464.1"
                     /translation="MNTKKKFLFIFIFITFQLFSESYRDYRLDDLNYLNNGRKRIQNE
                     GRIETSAKSKNTDKDTGEEGSDTKFIYNSFITSKFYSQKGGDKEDLARRYATQANFAP
                     EFVGFKRSGNNQFMILASPILSYKENYHLKRSVDRVVDTEAILAWKFDKSNFSINIEG
                     GRGFQRLDAYGLFFNGITNYFESNLFWKPFGIKFSLLALSYNYKQENFTIRANATNDK
                     IFGGNLLFTSLPFINHLQIFNYLVVEPGQIAEKQYYQADKQFKPNGRFIYNGLELKTN
                     PFWKIDTELGLFFVKGYRDYAEYSYQQLNHISKTNAFLSYLAFNWNLNQLNLRLGGLY
                     STKDKSNAVDRAHNGYSSLLSDVRIFGGKSSFLLMENVNAKNGTLFRDFDSSIENRYD
                     TKGMQLFSLGFSYPLSASFQVSTILNHTNSNLGVGNEVIFSGMYRNSNTDPFSGFFYG
                     SLCIAHVNPVTERKIIFDELRVDPTNKEFLRLYFASGIHF"
     gene            complement(23196..24137)
                     /locus_tag="PLF66_05175"
     CDS             complement(23196..24137)
                     /locus_tag="PLF66_05175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63465.1"
                     /translation="MLKTLSNSGKFLSSPVSFLLICLWIWNDHIGKWEFHNTITGKIS
                     DITGLIVFPFLLTALASILIRKKELEKILFFFANLSIILLFFIINWDQEWNNWIYANC
                     FGNQNGFADKSDLLCIPLCSFVNYFFYKKYSREQSEPNRKNKFLHIIAIILASVAFIN
                     TSTGDKKDYVAQDSYKNDDISQEPISSTLEAIYPFKYNYNFFIEEDITFSWYTKGNYK
                     YFSIFIFKNHKVEKDFLLTSSQLKKRITKSYGLIYFYTIESFLSFGNYEYSVLGSNDL
                     SWSKNIVRDQLNTMIKTEQCNRINISCSSINFISIVE"
     gene            24285..24878
                     /locus_tag="PLF66_05180"
     CDS             24285..24878
                     /locus_tag="PLF66_05180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63466.1"
                     /translation="MKKLLLFVILTNMISCYTYVNDELPRIRAIKPKIKIPAISFRIT
                     GIDDKDSLKEYNRIIRKKLENSNLFNVVNVIDVNDKLDKADAHHLEIHLKKQDVFENR
                     WFLGASAFVSLWTVTLVPAFEKNKVAMLVDYYVDGKLKRYDRFYQSSTRLFGIIPAVF
                     RVRNGDSVENPDAIIVNNLADNALYYLNELNFSESVQ"
     gene            25045..25629
                     /gene="hisB"
                     /locus_tag="PLF66_05185"
     CDS             25045..25629
                     /gene="hisB"
                     /locus_tag="PLF66_05185"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004477621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HMZ63467.1"
                     /translation="MSSETRKTSETDIKLKLNIRGKGEYQFDTEIPFFEHMLSHIAKH
                     GHIDMDLWLRGDIGIDCHHSVEDTAILLGATLHKQLGDKKGINRYGHFTIPMDEVLTT
                     VAVDLGGRFFFQYTGPKLEGKFGIYDAELSLEFLQKFALNAKMNLHVIVHYGENRHHI
                     HESIFKGLGRALRQALAFDDDSLGVIPSTKGMLE"
     gene            25626..26261
                     /gene="hisH"
                     /locus_tag="PLF66_05190"
     CDS             25626..26261
                     /gene="hisH"
                     /locus_tag="PLF66_05190"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002975172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HMZ63468.1"
                     /translation="MIAVIDFGMGNIHSCLKAISLFTNNFKLTTDPKDLEKASGIVLP
                     GDGAFEKAMSNLRELKFIDPLHNALERKIPLFGICIGFQILFEDSEESTEKGKLVKGL
                     NLIKGHVKKFSLRNYKVPHMGWNKLIINTKKKSILLNEVQNGEYMYFIHSYRPVDADQ
                     TSITANCNYAEEKFPVIVEKENIFGTQFHPEKSDKEGLKILRNFIRLANSE"
     gene            26273..27004
                     /gene="hisA"
                     /locus_tag="PLF66_05195"
     CDS             26273..27004
                     /gene="hisA"
                     /locus_tag="PLF66_05195"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016549913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HMZ63469.1"
                     /translation="MLIIPAIDILDKEAVRLYKGDYSQKTVYSSRPWELVQGFNKNGA
                     ELIHLVDLNGARSADSTNKDCIIRIRKEAKVRIQLGGGIRDMEKLKYYESLGIDKFII
                     GTSAVTDPKFVDEAIAFLGTDRIIIAVDALDGFVRVSGWEVKTKVRYEELLTRLNSQG
                     VTQIIFTDISHDGTMDGPNLNSYKYILDNFDFQLIASGGIASLKDIIDLTDIDAKRPL
                     YGIITGKAIYEGKLDLKEAIDNTRK"
     gene            27051..28301
                     /locus_tag="PLF66_05200"
     CDS             27051..28301
                     /locus_tag="PLF66_05200"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HMZ63470.1"
                     /translation="MNTDNLKKYNLIGIIVTIIGLSLCIMLIQEFFGSAGAFAESLCS
                     ATGDGDSCSKVAKSTWSGFRGVPILGDIAVAHIGFTFYGLCGFLFFSILKSNEEDKKG
                     FVSVLFILSIMAILIDAILLLISVTQIKTVCILCALTYGVTLSIFALSYIQIKSLSGQ
                     EGIMNQVKANVGKNITGNILNYTIAVLLFYACGIGVSRLSNSGSNKPEVDPESNKQYL
                     QSKIDAFEKRSPVTINLSQAPTIGNPNARVTIVKYADFNCGHCMHTSHILRQLLAEFP
                     DQIKIYYKNFPLDGTCNPLVQRKDPSASSCIAASAALCANKQGKFKEVYNGLYTDTEN
                     GIRHTISSVREIATKSGVNVQQLQTCMGSTEIQNFILQEVKEGEVLNIQSTPSLYVNN
                     KPLDPGTPNPNFLKALVEHLLAKK"
     gene            complement(28642..29721)
                     /gene="mnmH"
                     /locus_tag="PLF66_05205"
     CDS             complement(28642..29721)
                     /gene="mnmH"
                     /locus_tag="PLF66_05205"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF008752.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HMZ63471.1"
                     /translation="MIHLSKTKTIEEILNEYSSNSHILIDVRSESEFAEDHVPGAINL
                     PVLNDKEREIVGTEYKKNPFTARQLGAKLIAQNLKGNLEKVEELISVFNKGSDDKHKP
                     FCHFVVYCWRGGMRSRSLFTVMDLIGYKTTLVDGGYKAYRRRVNFFFHETEQLDTVCI
                     YGPSGSGKSELIERMIKFQLPAVHLEKYAKHKGSLLGGDMINQPSQKMFESLLFHALS
                     KIQSSYVITEGESKKIGRLLIPDRFYSKMTSGKKIWVELPLEVRANRLAEEYNFPVDY
                     ILDKIKFLKQFIDGKIFKAIAEAIVANDRYTAAYLLLKHHYDAYYLKGSPDHNTSREY
                     LEVFKASSFEQLNEYVVQFFKQSSF"
     gene            29785..30936
                     /locus_tag="PLF66_05210"
     CDS             29785..30936
                     /locus_tag="PLF66_05210"
                     /inference="COORDINATES: protein motif:HMM:NF022825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3187 family protein"
                     /protein_id="HMZ63472.1"
                     /translation="MIKFKKYIIFFTILLFLFIPKNIFSFMDYPYGVGFQMIQIPLLP
                     IDNVNPGQGKFSMRVSGRLMNAFSLQTNRFIIDGEEGQIEPSLRYALSDYTQIGFSLP
                     FMARGGGFLDHSIETFHRITGVTQGGRDHYPRNQFNISYEPLAQYYPLFDSTPLQAYL
                     NRNFDQREYPRNSNDQPIQFSKQNDLIHNLVINKYYPYLNEYRTEDYINNFNSVARGN
                     PKLFFQTTVLKGSFWFDKITIGNQVRFATKTSELIGTPGNDVSIFGIYHKDWFDGRVN
                     WKLGVSYTRFEFAKYRNLDLRKDEWTIRPSLTYNMDENWKLHFEYVYFPSTILRFGRL
                     SEPAHQIGIGTSYKYKDYQLQFALFENLITYSITPDIGFLLSIEKTTFN"
     gene            31228..33741
                     /locus_tag="PLF66_05215"
     CDS             31228..33741
                     /locus_tag="PLF66_05215"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HMZ63473.1"
                     /translation="MRAFQFIFLLLFVFPIFGNSFKIDLTQICQSGKCDPRIWYSTDS
                     FENNFVKLETPPADWKQADKFPIWMNKHYTKEGSLATYSLLTFFDLPDNVTLNRQTGI
                     RLGEIGEVFEIYINGNLIIQEGKVNDHEVIFHRTVRGKIYEIDKTILKNKNNVLLIKL
                     SGHPKYDHTGFYLTKGYEIGFFEDLQYDEQDRITLILIGIYITIGVYHLFLYTKRRKD
                     FYNLYYALYTIGLGIYIYTRTSAIFEHPWDTTLIQRVELIVLYPVIFFNFALQEELFF
                     QRIRKVTKYYGYFLFLWSFITIFIPMYIAEYLLRFWQLTFITLGIGIAVNIIYTSVKN
                     KIPNAKRLMTGMIILTFSAVFDVMDSLVLNTGLAFSKYAFFIYVFGFATILANNFIQV
                     HNEIEHLNDTLERKVIERTKELTNSLEKVNQLKKQQDGDYYLTSLLLNPLGVNHAIKK
                     GVDIEFIIQQKKKFTFKGNDSELGGDLCRTETISLKNRQFTVFFNADAMGKSMQGAGG
                     AIVVGAVFDSIIERTKLSKVVQNYHPERWLKNSFVELHKVFESFDCTMLVSIVVGLVD
                     NASGLIYYINAEHPFPVLYRDGKASFIGDDFIYRKLGTPVNETAYISVDTFQLKHGDI
                     FISGSDGRDDIIIKNSLGEKVLNSDEKFFLRVVEDANGDLKTILQILKREDELTDDIS
                     LLRIYITENPYPFDMSHLEDIINQKSIIDIEDNKDLPPKELYIRNLKNKFFINIKTNK
                     YEEAIQAGKEYLEWIPADDEMISKLSETFKNAGRIDESIELGERLRLRRKQNIPNLLN
                     LAECYRMQNQIERANLLLKDVLAFDKENKIAKDMISKLV"
     gene            complement(33733..34320)
                     /gene="tmk"
                     /locus_tag="PLF66_05220"
     CDS             complement(33733..34320)
                     /gene="tmk"
                     /locus_tag="PLF66_05220"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HMZ63474.1"
                     /translation="MMKPKFIVFEGIDGSGKSTLSSMVLSTLLERKIPTIKFNEPTQF
                     ETGIKIRQFLKKEISLSREEQIELFLADRNDSLERNVYPNLRAGTSVILDRYFYSMAA
                     YQSDSEISPEEILLMNTKRNYPKPDLIIYLDINPEISLARTRRRNANEHFENLETLRK
                     VRIAYEKVIPPDALRLDATLPQAKLKELCITHILN"
     gene            34502..34918
                     /locus_tag="PLF66_05225"
     CDS             34502..34918
                     /locus_tag="PLF66_05225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63475.1"
                     /translation="MASVEPNDLISILKEKRVDEYRIIEQKKMISQISEEEIQSKYSN
                     VFTSIQDIDDILLRKGVYFALLGAIYAFHDRELNPLEAQHFPLFQVIKNDILKVYNEI
                     SDSLVDEETWLFETFDYGIKHVYYLDWQLYLSKICY"
     gene            complement(34915..35670)
                     /gene="map"
                     /locus_tag="PLF66_05230"
     CDS             complement(34915..35670)
                     /gene="map"
                     /locus_tag="PLF66_05230"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002176166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HMZ63476.1"
                     /translation="MVHIKNKAEIELMRAAGKLAAEILQYIEPFVKPGVSTLELNDIC
                     HEYTIKRGAVSAPLNYKGFPKSICTSINDVVCHGIPKKTDILKDGDIINIDVTPILKG
                     YHGDTSKTFMVGKVKPEIKKLVSDTEKAMWIGIEQVKPGNRVNDISNAIDEFLTANGY
                     GIVRDLMGHGIGKKFHEDPQIPHFKQTRLLTKLEPGMTFTIEPMVNLGSYHVVVSKSD
                     KWTVRTKDGKWSAQFEHTVLVTDKGYEILTLPD"
     gene            complement(35683..35868)
                     /locus_tag="PLF66_05235"
     CDS             complement(35683..35868)
                     /locus_tag="PLF66_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011670271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63477.1"
                     /translation="MSEEKSILIVSSKVKEYIKSKELMTSSDVIEGLNEKVYRLIDEA
                     MERTKSNKRSTVRASDF"
     gene            36089..36304
                     /locus_tag="PLF66_05240"
     CDS             36089..36304
                     /locus_tag="PLF66_05240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63478.1"
                     /translation="MELTTKFFNFILGLSGQAMEEADRIKRSLEKEYSRILSIGANNN
                     SGPAIALRETTETVIIFFEDLKFKLNL"
     gene            complement(36398..39805)
                     /locus_tag="PLF66_05245"
     CDS             complement(36398..39805)
                     /locus_tag="PLF66_05245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63479.1"
                     /translation="MGSELLFPFIASVIVILLFRKLDRSNYRLSQIKKHSAKMNEDIN
                     QAAMAGIQAVKDTTIDLEIMGKQARKVISDLEAKANETKSLMESLKANKEYLDSISAD
                     LKNVVTLASEIRHEAEYIQEGMQIIQSQRENIKDVEKEVREVREEVNGIVRTFSQTLS
                     TRTQDILESLATKIVELESLLEAKSDKVDESLNSVLNTFKEKLKTEVEIMIEETVGKV
                     EVANAKLDDYNTFVRESEKSLEIKITRYRDSSEAIAEKIEKLDTRFEEKAESVALIVQ
                     DKLGFFEKKFQDRFDSILDQVNQGKEAILGGLRMEVDSIRSEIESMSLETMTRRDELL
                     NDTRRQADSIVSNIQLFQEKYLEADNKLLREADFKKAELLKEIVKFQDEFQRIKETFH
                     EETENKKDSIVENLKNFESEMSRVANQIEHNTRDRFLSLKNELEDSLVTLHGKKKSEI
                     LDDIAAVELKIRELGKDTLQKIKTVDDYFFDLKNAMLESAKDIVKQVETEVNSVVQIL
                     DKEKMKTDGKIEHFVEEWNLELEKIKSRTQRDMDSLIERLKDIHIEGRELADIFKNEF
                     NTGKAQLDALLKTHTEALTAQRDGIITEVQGKVKKSQDEVDSVLGRIQKAGLNLYEKQ
                     ESLLADYGEKLYRDLQNKLEKVRYESEELLEDIQKAGMNLLEKQEEKIDKLTQTIDER
                     ISRQLTVLLDKGQLQLGKLESRITSYVQDVKQNIEQTLKNAKEDSDRQISSFNSQIQK
                     TFRDIEKANNAFLETNRDEFSKTREEFVRIKNSIDTDLDKVADIKKGLSEYVEAETNH
                     LRDTLDKITSKVDEIQSYSGLFDKTRQMIKDSEQTMRDLGSMLEQLKQEGGTANQFLK
                     HAELIKATKKEMEAELRLLETHKLRIDQIENELARATNVCDLINQRTDELHDKISMIT
                     SVDQKLIEIERIQNELEFKLSEVKIVNERINDLTQSLNNSNKSTFEITDRVQKVMRAV
                     EKIESRESELEDGINHMEEKIQSLSARNLDIKSIESKFDKVENLMVDLSARHKQIATM
                     QKRIETLKMETEEMKGGFENLLAEADDKFDKLSDFLVVVDAVTTTSAGKNFSKNFKLD
                     KDTTEILKRKKATVLSLYEKFDWTSDTIAQKLNLEKSLVDAIINNKS"
     gene            complement(39931..41220)
                     /gene="aroA"
                     /locus_tag="PLF66_05250"
     CDS             complement(39931..41220)
                     /gene="aroA"
                     /locus_tag="PLF66_05250"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012389537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HMZ63480.1"
                     /translation="MLVSKFNIPADFTIQVPGDKSISHRSVLFSSLAKGNSEINGFLE
                     AEDPLNTMKSFSKLGVEFTKLGKGSYKVVSPGKLGLTSPKEELDFGNGGTGIRLSAGL
                     LSGLVGIQTQLTGDASLRKRPMKRIIDPLSLMGADIQSLSGDGKAPLKIQGKKLISIF
                     YKSPIASAQVKSCLMLAAISSDVGLEYEEEELSRNHTENMLQFLGGEIEFFTKTHFKM
                     KPPFAFSGAKFQVPGDISSAAFFIVFGLLAKSGSILIQNVGLNPARTGILTVLKNMGG
                     KIEVENERSECGEVIGDLRVYPSNLTKTEISSDLIPSIIDEIPILTIAGLFSKNGFEI
                     RNAEDLRAKESDRIHAMVVNLRKLGIEVDEQKDGYSFEQVKEIQPALIESFMDHRIAM
                     SFLILKTLLENDIKIDDDSWIDTSFPEFKSIVLRLKS"
     gene            complement(41381..41908)
                     /locus_tag="PLF66_05255"
     CDS             complement(41381..41908)
                     /locus_tag="PLF66_05255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein LipL21"
                     /protein_id="HMZ63481.1"
                     /translation="MSKSKAVLLFTMLIVAVSGCGTGDKEAHSVGEGGKQFEGWHGPP
                     EDVNKRPFEFYYMKSVGKASPKAMEKRSGAMMQSTCTDAATTKVKGDLIGKLVGESIQ
                     GASGVSDGESTGAVVVREFQGKLQGVNVKECKALYKDDPEIPYSGWKECECVVFIKVP
                     GGRDAVIAKAQELSK"
     gene            complement(42012..42710)
                     /gene="cysE"
                     /locus_tag="PLF66_05260"
     CDS             complement(42012..42710)
                     /gene="cysE"
                     /locus_tag="PLF66_05260"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004764119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HMZ63482.1"
                     /translation="MFDNIRKIKDNDPAAKSYLEVILCYPGLHALWFHSIAHFLYKCK
                     VPLLPRMLNYIARFLTGIDIHPGAKIASGIMIDHGMGVVIGETAEIGTGCLIYQGVTL
                     GGTGKESGKRHPTLKENVVVGSGAKVLGNIILENNVRVGAGSVVMRDVPANCTVVGIP
                     GRVVKSEEKNSEHMLDHGQMPDPVARVFSILLERIDTLQQEIAEISHEKSNKKDQRIV
                     EDKLLQNFIYGEGI"
     gene            complement(42770..45871)
                     /locus_tag="PLF66_05265"
     CDS             complement(42770..45871)
                     /locus_tag="PLF66_05265"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HMZ63483.1"
                     /translation="MKLSFKIFIVYLSFTLAILIPTSISVYLLMTQSVEEQIQKNLKE
                     TAEHMFDKMDRVLFERYSDIQTISADPIFLQPKVTDEEIAKKLISYRNQRKVYISLAY
                     YDKDCIKIADTNGLSIGEKCKDSLWKEEVYKDNRVSAASDILYLEELGNRVIVFAAPV
                     LSTEGNLIGAVTAYVSVGRLHQIIQGTKDLEKYLLIDLLDHEGNTIYSNHKVQKKNSV
                     LKQTETEILASANETIFAKESENGFLDFQGNRWQLYLQVPKKEAFKAITKIRDTGLTV
                     GLILISFSLAIVYFLSRRVIAPLLVLKKAADRFGQGKLDERVPAFSSDEIGDLSQTFN
                     KMANDLSQSLTELERKNVELEKLDKLKDEFLSNTSHELKTPLNGIIGIADSMIDGATG
                     KLEKEPVKNLKMISDSGKRLTNLVNDILDFSKLKNHEIIIQQKPVDIHSIADMVLLLS
                     RHLLGGKRIQLLNQIPEDVPAIKGDENRLQQILLNLIGNAIKFTESGKVEITTKQKME
                     DKLTIVVTDTGIGIPSDKFDSIFQSFEQVDASISREYGGTGLGLSITKNLIELHGGKI
                     WVESEVGKGSKFFFTLPISDEKPVDEPAKTYSLQKLEANEEYAQNEIIINTTAEGSIK
                     ILVVDDEPVNVQVLENHLTLAKYSVLKASNGEDALAILEKEEVNLILLDIMMPKLSGY
                     EVCKTIRRKRSSGDLPIIMLTAKNLISDLVYGFECGANDFLAKPFQKEELLTRIKTHI
                     QISTLNKSYGRFVPHDYLHFLSKESIIDVNLGDNVAADMAVVFSDIRSFSTLSETMTP
                     QENFNFVNAYFKRVSPKVREYNGIIVKYVGDAIMAVFKEKTEDAIDSAIAQLNELSIY
                     NKKRVANGYLPIHTGYGIHSGFMMLGMIGETNRMQGDAFSDHVNLTSRLEGLTKYYGA
                     SIIISSIVLGKIQDQSKYAIRYLDRVRVKGRKQPIEIYEIMNADDPEIREKKMQTNAD
                     FKKGINFYLYGDMKSAGEVLSELHRSFPEDKAVLQYLERTREYLKHGLPLDWEGVYDM
                     KSK"
     gene            complement(45868..46965)
                     /locus_tag="PLF66_05270"
     CDS             complement(45868..46965)
                     /locus_tag="PLF66_05270"
                     /inference="COORDINATES: protein motif:HMM:NF024772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HMZ63484.1"
                     /translation="MRIINLICLIIIILFLACSKEKEGNAKIRLGVPLRVSYLPVFIA
                     EEKGIFKKNGIDVELHIDENLSELYAERKLDIICTGLTESILSTSEGHFSKIIYRFTY
                     SITNDLIVSNNSIKDMESLKKKKISFEGINSSSHIFVQQLLSKKGIQDGEYFAVNLPM
                     HKVIQELEKGTIDAGHTTGVSLSEINKKGLSVIGKSSDDPDLLADTLVADSAFLEKHK
                     QKVKLLVSSIIEAKSFYDSNPEEGVSVFVNYSKRKKEDIKEELSGIKFLSQVENEAAL
                     EKQDDKLQQTNINESPKFDFSQGPKDGAKPPGGILPDQSISMNQKEKRFGLLTAGQKI
                     IQFLRERGQLYRMPDLQSIIDDQFVKAGGDK"
     gene            complement(46952..47548)
                     /locus_tag="PLF66_05275"
     CDS             complement(46952..47548)
                     /locus_tag="PLF66_05275"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HMZ63485.1"
                     /translation="MKKILIVCLILSGMQIIADPLADSYGQEAKREFAKALETMEGLS
                     KDNPNEYFYQLRTGWVALIKGDFTKSASYYQKAALLAPNAIEPRLGNVRALLAMGKYK
                     DVDVACKTILKLDPKNYFARSTLAYANYVLANYAEAEKQYESLIVDYPADTEMQIGLG
                     WSLLKQGNKAKAKTIFAALTKIIPKEERVSSGAYYANN"
     gene            complement(47548..49404)
                     /locus_tag="PLF66_05280"
     CDS             complement(47548..49404)
                     /locus_tag="PLF66_05280"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HMZ63486.1"
                     /translation="MDIKKVWLIGIILLTLFNTSSVLSQTVQDAIGQMEKLNYKKSIE
                     TLDKVIKKNPQDSVAYAYRGYAYMMINDFSKAYDDYKKADELYSTLETMSGMQWALLA
                     SGKYNESISVGEKILEKDKDNFYAKFRISEAYKAKGDYVKTIELLDQLIQKYPDSSMA
                     YLYRGYAYMMTEKYDLALADYKKADSLYSSLDSLAGIQWALLAMGKNDESIAAGEKVL
                     AIEPNSYIAKQRVADAYMEKKEYSKASERYKQLESQFGKNGDLLWRIGLCEYYMGNKE
                     EATKLFEEGKKMSPDHKGIQYSLSGASVYPYFAVIPEASVYNFNGSDYVGSGSKFGLG
                     VSYSPNAGWNLRANVTHETTQNLNSTNGVFNYIIDPVNIAYLSSYGYLRNPSTIPSYY
                     FGNFTNVNNLYSLISSNDYSTTKYALGVSYQLSDRLALHFTPQGLSSNTSFLNKGAAG
                     QFGVSYTNKYTISFYGAAISAPYSKGGQGTVTFYYPFTENFYSSSTISGQAMTVKESE
                     VVWISVRPLLTSTDSIKVSKNYGFFQQEFGYLWKYFYVAIGGRYGTARTPFMGENWIY
                     TGFDLHSGGYGQIGFKSDRLTAQLMLSHDQWLDFRNDRPTSNTAKLTIVWRF"
     gene            complement(49380..51488)
                     /locus_tag="PLF66_05285"
     CDS             complement(49380..51488)
                     /locus_tag="PLF66_05285"
                     /inference="COORDINATES: protein motif:HMM:NF015226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015204 - urea transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071918 - urea transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea transporter"
                     /protein_id="HMZ63487.1"
                     /translation="MNFFKTLYFSILSSYSSILFSSSPVLGLFIFLATLFNYEAGLLG
                     LLGIIFAVLLAKFLGVHEERIKKGSLGFNALLVGLSINLVHSVDTTLVFILFVSIVLL
                     VFLTIASEYAFSYFLGLPVLSIPFVIVSIIVYFSFYDYNGLKYRVANTPFPLDIYFPE
                     LPSYVSYYLKSLGGIFFQSSPWAGLCFAIILFASSRIAFVLSVIGFAAGISFHLALEG
                     KMGNISTGAVGFNYILTAIAVGGIFIVPSPSTFIVAGLASIASALVSSFASVLFTPYS
                     IPILALPFTSVSLLFVYSVKLLRNRRIVAVDFIPGAPESNLDYYKSRLQRFGVSGLYI
                     RLPFSGKWKVSQGFNGKFTHRDAWKESLDFMAIDAEGKVRKGKENNVEDFYTFGLTVL
                     SPCAGRVIKVVKHLEDNKPGDVDTKNNWGNFVIIEHTPFLFSHLSHFSKNSISVKEGD
                     YVAAGTKLGLAGNSGRSLEPHIHLHFQSTPEIGSSTIPVTFTQYQNESNGRTLIHFNE
                     IPIEDQVIANLHSDFNLKNFYNLSPGEEINISLTKNESKIITEKWKIQLDLLGNRFIE
                     DEKNNRVYFYISADYFSCLDYIGKKNTGLFFFYLANYRIPFINISSLWKDRMSYKYFS
                     NLFIRLAKDLIHPFSDSVGFEWQGEMIEKDTNLNFKIKVIKNINTVLYELDLLPDSTA
                     PGSIQIKNNEGDQWILKKFG"
     gene            complement(51498..52829)
                     /locus_tag="PLF66_05290"
     CDS             complement(51498..52829)
                     /locus_tag="PLF66_05290"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF014803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HMZ63488.1"
                     /translation="MSKRMYVRPVIRKQYMGAANKFGGFNIPETMHSIDGISIDSLVE
                     KFGSPLFVFSEATIKKKIADYKQAFESRYPSFQPTWSYKTNYLNAICKVFHKEGFWAE
                     VVSSFEYQKARKNGIPGNRIIFNGPYKPYDALKLAVSEGARIHADHLDEIRDLLKISE
                     ELGKRVDVAIRINMDTGIYPLWSRFGFNLESGHALEAARRIAHSGGKLALVGVHSHIG
                     TFMMDASAYKRAAKKLTDFYKKVRDELRQPLQYLDMGGGFVSANKLKSTYFSGSSLLP
                     SFDQYAEAICSSIYDSFAPEEPPPLFLESGRALVDESGFLVSSIVGSKTMPNGKRALV
                     LNAGLNLLYTSTWYEFKVSPVKDYSKAYEDVTLYGPLCMNIDVVRESCLLPNMSRGDH
                     LVIHPVGAYNVTQWMQFIEMRPAIVMIEQNGNVKQIRRRETVDDLNSIEME"
     gene            complement(52822..53889)
                     /locus_tag="PLF66_05295"
     CDS             complement(52822..53889)
                     /locus_tag="PLF66_05295"
                     /inference="COORDINATES: protein motif:HMM:NF026968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HMZ63489.1"
                     /translation="MSRLVVAVSGMNAVDSPGPGVPVLRSLRESKLNPELVGFAYGAL
                     EPGNFMSHLLESSFLLPYPSSGPDVLLERIMHINSIKKIDVIIPTLDSELDNYISISN
                     QLNNAGIKLFLPGRKELHTRDKTFLKESLEDAGVLLPETISVQDVSAIRPVTDKIGFP
                     LFVKGVFYEAYLAHNYEEAVGYFYAMAGKWGVPVIFQKYIAGEECNVCALAKEGEMIG
                     AVVMKKLFLTDKGKAWAGVTINNEEAKSTSKKILNYIKWNSGCELEFIFENQTGKMYL
                     LEINPRFPAWVYLATASGQNLPDALLRLSLGETVTPFSDYDVGKVFVRHSWDEIIPMK
                     SVESLTIKGHLLSNDKGDKNE"
     gene            complement(53886..54131)
                     /locus_tag="PLF66_05300"
     CDS             complement(53886..54131)
                     /locus_tag="PLF66_05300"
                     /inference="COORDINATES: protein motif:HMM:NF017239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr-rel-A system PqqD family peptide chaperone"
                     /protein_id="HMZ63490.1"
                     /translation="MASDALKNLALSDTGFVFDPTTGNTYTLNETGLVIVRLLKQDKT
                     KDEIIKSILAEYEVEQDEVERDYSDMIIQLTELGFIR"
     gene            54427..55419
                     /locus_tag="PLF66_05305"
     CDS             54427..55419
                     /locus_tag="PLF66_05305"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HMZ63491.1"
                     /translation="MNTQTDWGKLQNLQKTFKRGSVVIKEGTSPNALYVLMSGRLNVI
                     RDGIKISQIKKRGEYIGEIAVLLNIPASATVKAETECVLIEIESSKIIPFLHHAPEIA
                     IALARKMAERLVELNANFSQLIDKSYRPDFIKSLQNRQKLEITIKPEYEINLEKLRSL
                     FVKYAAGMDVLTQGQYPRALYILVSGTLDIIKNGKVIAVEDKPGYYLGDVAVLRNTFS
                     NATVTTRTESTLIEIGIEKVEAFLNHKPDIAISVAVKLAERTLAINELFLDLQTDAIK
                     EYKKKEEQLEKKNAVNSSNNTAGYKKIDRLSDEIEKLRKEGQEIYRMLGIESIE"
     gene            complement(55490..55981)
                     /locus_tag="PLF66_05310"
     CDS             complement(55490..55981)
                     /locus_tag="PLF66_05310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63492.1"
                     /translation="MNTTSPVTEITFNCPHCGKKSKLANSISHSNVFTVSCYFCHGET
                     DVRCKTDSNGAKSLIPEGPVPTFHKIELSFLPEETPLLKSPSAYASEKTYQPVILNMN
                     DTSSEMESIHLETEQIDNRQQEEKKGFLKGLRDDLVEMLQIQTVETDSDIPIFSVVKQ
                     RKS"
     gene            complement(56076..56258)
                     /locus_tag="PLF66_05315"
     CDS             complement(56076..56258)
                     /locus_tag="PLF66_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002975080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63493.1"
                     /translation="MENKEQDNFWGLPIAREVTTEAFIKDFWDPSTEEIIVPKHFLGV
                     GWSVNLYAVAKKIGVI"
     gene            complement(56579..57664)
                     /gene="carA"
                     /locus_tag="PLF66_05320"
     CDS             complement(56579..57664)
                     /gene="carA"
                     /locus_tag="PLF66_05320"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017857077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HMZ63494.1"
                     /translation="MKAFLVLDNGEVFEGESFGYEGSEVGEVVFNTSMAGYQEILTDP
                     SYCKQIVTLTYPMIGNYGICAEDMESDKIQVSGLIVKEYVPVPSNFRSTETLSAFLIR
                     HKIPAIQGIDTRKLTRFIRTNGSPNGGIFIADKYSDEFLKKVKDFPGIKGMDLAKVVS
                     TKERYEFGSKENKPYRVAVYDYGVKTNILNCLNKAGFAVTVFPAEYPIEKIMEENFDA
                     YFLSNGPGDPEPVSYGIHSAKTIIEKKKPLFGICLGHQIIGLAMGRKTTKLKFGHRGG
                     NQPVKNYSTGRVEITAQNHGFAVVGEDSSAEPITHINLNDQTIEGIRSSSLPVMAVQY
                     HPESSPGPHDAEYLFTEFFELVKKSKS"
     gene            57708..58055
                     /locus_tag="PLF66_05325"
     CDS             57708..58055
                     /locus_tag="PLF66_05325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63495.1"
                     /translation="MLSPKDDSFTLIFGHISDTNLIPILINEKKYLVPKHVNLLRAFH
                     YIAATSEEYDLKLQKHCWAGSCENCKCSFKDSQLGEAEGLACQMDLEVNLKIISLPKT
                     MKRKIKFAAEYQI"
BASE COUNT        19459 a        10225 c         9112 g        19276 t
ORIGIN      
        1 tttctttgtg ctcacaggag attgatcact aaaaatctct ctagaaggtt ttcctaacca
       61 tgctgcaata attttttgtg cttcttccgg aacttggtct tgctcatcaa tccaatcttg
      121 gattacatca atggattcat ttagatattt agctgcttcg gcaacagttt tattttgaga
      181 ttccataatt ttcttgagat gaactcccca tcttgcattc tctccagctc cagctctgaa
      241 aaaagatttt cctgtgttat gaattttttc ataggcttgc tcggataaaa cgccagagat
      301 aatagctaga aatgaaataa aaaatggatt gagtgtttca tacattcgat tggtttcatt
      361 tcctccatct gagattacta actgtcctga ttttgctaaa acaaaaattg caatagcagt
      421 caccattcca agaaacggac ggaatatata ccaattgatg gaatgttttt cttgttctcg
      481 aaataattcc cttgttaaaa aaattacact tccaagagaa cccatagaca atgtaaggat
      541 aagagtaagt agctgcgcag gcatagttgc taaaatatca aaacgaagct ttgtcaaata
      601 acgcagctcc actagaaaat ctctaagctc atcattatcc attttagcct cttccgcttc
      661 tgcaacttct aattcttcta cagcttggag gagtgcatgt aattgttctg aaatttcttt
      721 ttcagactga gctaaattct tttgatttgc ctctaaattc gttatgagtt tatcgagttg
      781 ttttaaattt tcttcatgct tttctgcaag aatttgtaaa actttatcag gattattgat
      841 attagccgtt gtaggagtat ttacaggtgg aatattttct ttttgttctt ttgtttgatt
      901 aattagattt gcatattgag ttttaacgtc cttaaattct gcttttaatg tatttgctaa
      961 atctataaaa tttgaaggaa aagttgttcg ttctgctttt aaaagcgtta gttttcgatc
     1021 tgactgttct aatcttttta gtaagtcgta aattctagtt ttaatttctt tgatcttaat
     1081 ctgatttttt tcatattgat tcgaaagacg aagtagcgca ggaactgaat aatttttttt
     1141 agtttgctta tccactatgt tagtaagtct ctgctgaaat tggtgagaac tcaaggtccc
     1201 taacactagc caagttgtaa ttacatacca aagcagtaac caaccgataa tcgtaagaaa
     1261 acgtgcacct gtcacagttt tattcattaa agaagacaaa gttttccaaa atttttccat
     1321 ttctccaccc cacttaatat aaattcaagt tcagtttctt agagttccct agtgaaacaa
     1381 gtattaaaaa agacaaattt gattcgaaag ctttatttag cagataatgc tgagtttgcg
     1441 taagtagatc gggtctttac gaaaaaggct ggaaaaaaaa atgagtaaaa ataatatggg
     1501 caaaatagtt aaacatcaaa ctatttttca aaggagatta tcaatgaaca aaattctttt
     1561 atttacactc gttttaggat tagcttactg tgaaaaggca ccagaagcac caaaagcaga
     1621 aacatctaaa cctgcagaaa ctgcaaaagt tgaaggaact tccgttggat tagatttaac
     1681 aaaatctagc ttaaaatgga tcggaacaaa attaaaaggc aagcacaatg gaactgtaaa
     1741 aatttctgaa ggcaatatcg ttgtaaaaga caacaaggtt gtaggcggaa aattttcctt
     1801 agatatggca agcatcgaag atttagatat gaaagatgat ccaaaaatga aagctaaatt
     1861 agaaggtcat ttaaagagcg aagatttctt taacgttgaa aaatttccaa aagctacatt
     1921 tgaaattact tctgtaactg aagacgcttc tggacaagcg actattactg gtaacctaac
     1981 aatcaaagac gttacaaaag gagtaacttt tccagcgaaa attacttacg gtgctgacaa
     2041 aaaacctgtg tctgcaacag ccaactttaa catcaatcgt caactctggg gaattagcta
     2101 tccaggacaa cctgataacc taatcagaga tgaaattaat tttgatttaa gtttagcaac
     2161 tttataatat ttcattctaa ctttagggat tgtgttagaa tataacaaaa aggaaaactt
     2221 actccattcc tgtttcaaaa gttatactct atcacaatcc ccttttcttt ccaaaatcaa
     2281 agcttaccaa tttttaattc acaataatta aaaaatctag atcgtttaga ttttaatcag
     2341 accctaatat ctaaaaaaat tctattgtaa aaaaacgaat gtttagaaga catatactga
     2401 ataccaaata ttagatctaa atttctgaat caaagtgtgc ttggtgtaac tgtttttctt
     2461 ataaaatcat tttacgcaaa attctacttc aattaaatcc ttctagaaat ttaatctatg
     2521 tgtttaatat atatgtaaaa ttcatatcta gactttgata caaatcttat gccaatcatc
     2581 ctcgatttaa aaaccaggtt ccaacgtatt cttgaaaaaa gccgacatat atcaggtaga
     2641 tttatctgta tgcagcttac ctatatagta gatgcagaaa ttctaaatct ctataatatt
     2701 ttagagaaag aatatccaaa tattggagaa agattttgca tcattgcgat aggcggctac
     2761 ggtagaatgg aattagctcc ctattcagac attgatattc tttatcttca caatggacta
     2821 gatgaatcta tcctaacaaa tataatttct agaataaata catttcttta tgactcagga
     2881 aaagaagttg gtcactcttg tagaacgatt gatgaatgta gagataacct agataatatc
     2941 aatacattct atgctatgtt agattctaga tttttggctg gatcggaaaa attattttca
     3001 gattttgaaa accaattttt aaaaaatcta ccaatggatt tacttagttt tcataatgcg
     3061 gacaaaatta attatctaac tggagtggtt aactctgatt atcccctttt agttgcggaa
     3121 ccagatttaa agaatggatt tctaggtctt agaaatatac aggctgtata ttggatcgag
     3181 aaatcaacaa actacattcc ttctttgagt ggtctcgcct tattacctgt atttacgcat
     3241 ggagaagtgc aacaaattga aagcgcttat gattttttac ttagagcaag aatagcttta
     3301 cacttgatcc atggaagaag agtcgatcga ttggatttaa ccgaacaacc aactgttgct
     3361 gagtatatgg gctttggcgc aaaatcagaa ttaaccgctg tagacagatt gatgaatacg
     3421 ctttacacac atcaaaatga aattcattct tttttaggta tttatttgga ttataaaaaa
     3481 gctgatgcaa gtcaaatggt tacacttgga aaatcagaat tttatttaag acaattagga
     3541 tctgaccttt atcctcccaa agcgtctcaa cttttttcca atccagaaac tctatacgga
     3601 gacattctac aaatttttta tatagcacat aaacttaatt taaatttatc gcctagtcta
     3661 attggacaat tgaaatttgc ttctaacttt ttacaggaag attttaagaa ctctaatctt
     3721 gctatagaaa tcttcttaaa gattttaaaa gagtcaaagc aaataggaaa gattctaact
     3781 agtatgcaca gagctgggat actaggaaat cttttaccag aatttggagc ctgtgctaat
     3841 ttttctctat ttagttatca ccatcaatat cctgttgacg agcatacttt atttattctg
     3901 agagaattgg ataaactttt aaatggaaat tttgaagata aggaagttca agcagttttc
     3961 tcagaatgcg agcatgttta tattttagct ttatcaatac tcattcatga tgcaggtaaa
     4021 gtaaagttag gagatcattg tcaatacggc gcagagttag ctactgcgat aggcgaaaga
     4081 ttaggtttga atgatgagga aacagatctt tttaaatttt tagtggaagt tcatattcat
     4141 atgtctgaac ttacatctaa aagagacatc tctgatccat tgctattaaa tgattttgca
     4201 agtttgatag gaaacgaaaa tcgtctaaaa ctactctatg tatttacggt catcgataca
     4261 aaatcagtcg gtccaaacgt gcttacaaat tggaaaaaat ctattctata tacactttat
     4321 aaaaatacgt tggaagtgct tcgaaatagg gagaattcaa tactatctcc aaacacaagt
     4381 aaagatcacg aactactccg taattattta actcaaaaag aaaaaatcga agaagactta
     4441 agcaataaaa ttgtagagtt tgcctcttcc atgatcccgc atacctatca aagatacaat
     4501 actcctagaa gaattctaca tcattttatt ctacatacaa aatgcaaatc acatccagaa
     4561 gaaattccag aaatagagtt tgaaaaagaa cccgcttatg ttacaatcac tgtctacagt
     4621 cttgaagata gatatctact ttcggattta actggaacag taacttccga agcgttaaac
     4681 gtaattggta tgcgatcctt tcgaaatcaa agtggatttg taatcagcca aatacaaatt
     4741 acagatagct tcggtggtgg ggatattcga gaagaaaaat tagatcgact caaaagaaat
     4801 attaaaagtg taattgagaa aaaattaaat gtagaaaacc tattaaagtc tcctgttgaa
     4861 tgggtaagct ataacaaaat tccagatggg atggttgctc aaaaaataga gtttaacaat
     4921 gtgttatccg ctgattatac aatattagaa attttactac ccgattcact tggattattg
     4981 tatagaattt taaagcaaat tctatctttt gatgttcaat tgcattttgt tagaatttct
     5041 actagtgctg actatgccta tgattctttt tatataaagt caagcgcagg aaataaaatt
     5101 gaagatgtag aactcttgaa aaagatggaa caagaaatcg caaatgcagc ttcccagaaa
     5161 gtgcaagttg atgtttccta tatagaatac taattatgtg gtggaaagaa gcagttatat
     5221 atcaaattta tcctaggagt ttttatgact ccaatggaga tggaatcggt gatctccaag
     5281 gtatcattga taaactagat tatctaaatg gaacaacaaa ctcgctaggt attgatgcga
     5341 tttggctttc tcctatctat ccatctccaa tggtcgattt tggatacgat atttcggact
     5401 atgaggatat agatcctgtt ttcggcgacc ttaagacttt taaaaaatta ctaaaagaag
     5461 cccacaaaag aaatattaag attataatgg atctcgtgat caatcataca tcagatcaac
     5521 atccttggtt tattgagtct agatcctctc gcaaaagcaa aaaaagagat tggtatatat
     5581 ggcatgatgc gatagacgga aagcctccaa ataattggat gggagcgttt ggaggacttg
     5641 cttgggagtg ggatgaaaaa acccaacaat actactacca tggtttcaca aaagaacaac
     5701 cagatttgaa ttggagaaat ccagaagtta aaaaagcaat ttttaaaatg atagcctatt
     5761 ggctagatat gggagtggat ggatttagac ttgatgtagt aaattattac atcaaagata
     5821 aaaagctcag agataatcca aaagattatt ttaagggact aagaccttat gacattcaac
     5881 atcatatcta tgatagagat agacctaagg ttcatagcat tctaaaaagt tttcgcaaac
     5941 tcttagattc ctatgaaggt gaccgtatgt ctgttgggga agtgtatatt gaacctcctg
     6001 gcaatccaaa attagcagca tcttattatg gagaaaatag tgatgagcta catatggcgt
     6061 ttaactttgc atttctgtat tgtaaatggg atccaaataa gtttaaagaa gctatctatc
     6121 atttagagaa agaattaaag cccgatgatt ggccaaatta tactctgagt aatcatgatc
     6181 aaattcgtca tattaaacgt tatgagaaag gcgatgaaac aattcacaga atgaaaattg
     6241 cggctatgtt aatgctgact ctccgaggaa caccttttct ctattatggg gaagagattg
     6301 gtatggaaga ccaagcaatt cctaaagatg aaattcaaga tccactggga aaaatgtatt
     6361 ggccaatatt caatggaaga gataaatcaa gacttccaat gtgttggaac gcaaatgaat
     6421 atgcaggatt ttcaaccgcg aaaccctggc ttccaataaa taaaaactat aaaaaaataa
     6481 atgtggaagc agaatcaaag aaaccacata gtctgctaaa tacctataag aagctaatcg
     6541 ctattaggcg tagctctgac atattaaaac ttggaagtat aaaattaatc gacgatcccg
     6601 agaataaagt ccttggatat tttagaattt ataagaagaa caaaatacta gttctgctta
     6661 attttaaaaa tgaaaaagca aacttagatt tacataatta tacacatcca aagacaaaag
     6721 ctacactaat ctactcaaca gatcataaaa gacatgaggg tgaaaatctt aaattgcctc
     6781 atattcactt gcataattat gaagggatga ttattcgctt cgaataacta tgatcaaaat
     6841 tcatttaaac aggagatttt tcaatggatg aaatagaact aatccgtgac tttagttttg
     6901 atgccgcaca tctattgcct aacgttccag aaggtcataa atgtaaacgt ttacacggtc
     6961 acacctttag atttaaaatt catctaaaag gaaaagttga tcccaagaca ggttggctta
     7021 tggattttgg tgagatcaaa aaagtagtga aacctctgat tgaaaattac ttagatcatt
     7081 tttatctgaa tgatgttgta ggattagaaa acccaacaag tgaaaacata gctatttggc
     7141 tctggaataa attaaagcca gaacttccac ttctgtacaa aataaccctt catgaaactt
     7201 gtaatagcgc ctgtgtatat atgggtcaaa agcatgaaag ctaaaagtaa caaaggagcg
     7261 ttagttctat tttcaggtgg tcttgactcc actacttgtc tattttttgc actagaaaga
     7321 cacagagaag ttactgccat ttcatttgat tattctcaac gacataaaat tgaaatttta
     7381 aaagctaaaa agattacatc ccttctaaaa cttccacata gagtcatacg aatagatcca
     7441 aatatcttta aaaacacttc tctagtgaat aaagaaatta aagttccaaa gaatttctca
     7501 tccaatgaaa agataccaaa cacatatgtt cctgggagaa atatcttatt tttgtcctat
     7561 gctacatcca ttgcagaagg tcttggttac gaatccattt acattggagt taatgcttta
     7621 gattattcag gatatcccga ctgtcgtcca aattttataa aagcatttca aaaggcgatt
     7681 acaattggaa caaaatcagg aaatgagaag aagagaatcc aaataaaaac ccctttactg
     7741 catttatcta aaaaagagat tgttatcatg gcaaaaaaac taaaagttcc tttcggacta
     7801 acacattcat gctatgatcc aatcaaaggc aaaccttgtg gaaaatgtga ctcctgcctg
     7861 cttagaaaaa aaggatttat ggaagcagga gtaattgaca aatgaacttt ttaaaaatct
     7921 tattgttccc catcctactt tttttttgta ttgattgtgc gacatattgg aacaatagaa
     7981 gaaaagactt acaagacgtt attacaattg gagctgaaaa acctgtgtat ggtttcagtt
     8041 taaaattagc tccagttcat gctggttttt tatttgtagg cggtgaaaca gaacctggaa
     8101 aaaaagattt aggcgatggg gctggcttaa gaggtggaag cttttcaagc tatttctcac
     8161 aacagctcgt atttggttgg cttggcggag aaacatttta tagcggcgaa ttagagttaa
     8221 atgatgaagg taaaattcag tatgaaaata aaataccttt ggtaaaagaa ccaagagata
     8281 atataaagag tcatgattta aaatatatag atataattag agatcctccc aaaaataggg
     8341 caaaacgtaa aaaagaagaa gtcagaagag ttataatcaa aaaagcatta gaggataatc
     8401 caaatgcaag tccaactgtt ctttcctatc ttccgaaaga aaatccaaaa ccgtttggtt
     8461 atccaagttc ctatttatat caaatagaaa tcacaattgg tttgtatggt ggtgttcgct
     8521 ttggattcaa tccggctgag ttgcttgact ttgttttagg tttcactact tatgatttat
     8581 atgatgatga taagaaggaa tgagatttaa atgattgaaa aaacaacact cgacttttta
     8641 aaaaatttaa aaaagaataa ctcaaaagag tggttcgaaa aaaacagatc tcattttgaa
     8701 aaagcaaaac aaaactttga atcctttatc actcaaatga tttttgaaat ggcaaaaatc
     8761 aatccagatt tggttggagt agagccaaag aaatgtatat ttcggctaaa tagagatgtt
     8821 cggttttcta aaaataagga accatataaa aataattttg gcgcagcaat aagcgagaat
     8881 ggcaaatcaa tggaaactgc cctcttttat tttcacttag agcctgggaa taaatctttt
     8941 attgcagcag gacgttatat gccagatagt aaagcacttc ggtctattcg agagaaaatt
     9001 ctggaagatt cctctgcatt taagaaaata attgaaagca aaaaaattaa agaaatattt
     9061 accggacttt cagacctgaa agtcaaaaca gtcccaagag gatattctaa ggatcatcca
     9121 gaaattgaat tactaaaata tacaagcttt atagttgaaa agcctgtatc tgatgaattt
     9181 attctctcta aagaatttac aaagctatgg attgaatctt acaaagcaat tcttcctctc
     9241 cttcgttatt ttaattctta attcatgaat ttttcaaatt tctttcttag ctttagcgca
     9301 ggtctctcat tattatttgc attcggtgaa tttttctcag ctaaagaaaa taaaagaaat
     9361 tattatctag gaggagtatt tgtaatttta ttcttttttc taatgcaatc cttcttatgg
     9421 tcaagcggat tactaaaact ctatcctcat ttactccacg tgcatacgcc ctttaccccc
     9481 ctaatcggtg ccttcctaga aagataccta ttaatgatgt gggaaaataa atcagaacca
     9541 attcagaaat ttttattaaa atgtattcta tctattagcc ctgtcctatt aacctttcct
     9601 ctttttttct ggtcgacaga gcaaaaacta gaatatattg aaacttgcta tacgttaggc
     9661 acaccgctta gatcaaagat agtgatcttt tttattatct ctgttctact gtttttcttt
     9721 ttaggtcttt taaagaaatt cattgcattc tttcgaagct ctactctcta ctcttccgca
     9781 aatcttaaac ttgttttagg aattttagct cttggatttg ttacaatcgt gattggcggt
     9841 ttctttgctg cccttggttc tcacgtgggc atggagatga acggttatat cattggatgt
     9901 tttttaattt tgctttatat tttgagactt aggaatccag aaatttttaa ggaagttcaa
     9961 aaaattgtag aagaagaaaa aaaatacaaa aactcccaat taaaatcagt agacctaaat
    10021 actgtttcag aaaaattaaa tcatctactc gaaatcgaaa aaatctatcg agaggataat
    10081 atcaatttga cggaacttgc cactcaaatt ggaatttcaa atcatcaact ttctgaatat
    10141 ctaaaccaag aactaaaaat ttcttttttt caattaattc atcgctatcg aatcaaagag
    10201 gcaaaggaaa aactactcgc tcaaccgaat gaaacaattc tctccatcgc ctaccatgtt
    10261 ggctaccaat ccaaatcatc cttcaatgat atttttaaaa aggaaacagg tctcactccg
    10321 acagaattta gaaaaaaatt caaaataaga ccagacttat caagtcgaac gaaaaaataa
    10381 gtcgcactcg atcgaggcgg tcgaaagctt tttcattccg tgctatgcta atctcattca
    10441 acggaggaaa ataaaatgcc ttttacatgt gagtttagtt tagaatgtgt cctgcaagca
    10501 ggatcctatc atatttttat gaatttcgtg cgctactacc cgattgctgg tttagctttt
    10561 gtaattttct atttatggaa aaaagatttt tttgcacgtt ttaaaattca ggataaagat
    10621 cctaaatcag agaagatctg gaatgaaatc aaacaatccg ctatcactct tacagtgttt
    10681 gctactatag gaactaccgt cttcaccctt atcaaacttg gagttctaaa atcacaaatg
    10741 tataaagatc caagtctcta tggaggtatt ccatatatga tcttctcatt tgttttactg
    10801 actgtatggc atgaaactta cttctattgg attcacagac ttatgcatca taaatctatc
    10861 tataaatatg ttcatcaagt ccatcaccaa tctgtaaacc caaccccaat cgcagcttat
    10921 aattttcatt atattgaagc tttctttgaa gctatctatg ctgtgatatt tacactaacg
    10981 gttccaatct actatcccgt ttttattttc catactttct atgcgatgat tatgaatatt
    11041 tggtggcact taggatacga atttttacca aaaggttttg ctagccattg gttttttaaa
    11101 tggattagca cttccactca ccataatcta caccatcaaa aattcaatgg aaactatgga
    11161 ctctacttta atttttggga tcgagtcatg ggaaccaact tcccatacta tgaaacgtat
    11221 tttgactcct tagccgataa acgagaacta aataagccac aaccaaaaga agcacattcc
    11281 actgctaccg cttaaaaccc tttctaaaaa atgaagaatt gaaatcaatt cttcattttt
    11341 tttgaaaaac cccgcacaga ttccttcatt ttttccccta ctttattaaa agaattcaat
    11401 tccaaaagtg gggtccctat gaaaaatatt ttttctaaac ataaatttac atttcccaga
    11461 aagaacctta cccttcatga tttcagttct accttactga ttcctgaaaa atattcagac
    11521 ctgtttcaaa aaaaagtcat tcagcataaa ggaacgcgtg cttacatcaa ctatcttctg
    11581 agaaaatacc aactctttat cgcaaatggt atgattcctg gttactccaa tgtaactacc
    11641 aaataccaag aaaaaggtca gaatcttcaa aaaattgcct ttcgtcctag accagaagac
    11701 tgggctgaac tgaaactata tcgtgttagt tttggtatgt ctatctctgc ctttatagtc
    11761 tacctactaa tagcagattc cgtagagttt gtaaacgccg tctcgaacca cctaaccggt
    11821 gtcggaattt caatgacctc gaatttcgat ttgatcgcaa aagtatgtct ttgcaataaa
    11881 aagacttact acacaactat ttttcaatat aggagaaagt atcacgacta ctaataatga
    11941 aaaatatccc aataccgatt gaataagatt ctaaaactca catccaagaa gttaccaaac
    12001 aattagacaa taagcgggaa aagattttag atgtgactga aatagaaaat aaaatgttga
    12061 taaaaacaat tcctataaga agataagctc acttacttta aaaaagatcc gcatcttaga
    12121 ttaattttat aagttagaat aattcttata actggagttt gtatgattcg tattttactt
    12181 tttttacttt tatctctctt cattggttgt gcatctggtg gggaaatgct agatgaaaac
    12241 aaagaaatcg ttagtgatag tggtggttta actcgtcagg agttagagaa tgcagccgat
    12301 aaaatcgcaa aggatattat tgtgaatttt aaaaaaaatc ctaatcctaa gggtgtagcg
    12361 ctcgctatcc tgcaaaccaa gaatgacaca tcggagcaaa ttcctacaga ggtttttgaa
    12421 aacaaattag tggcaacatt attatcagga aagattacaa ccattcgaac agataagaga
    12481 gcagagcagt taaaagagat caaattaagc cagcaattag gaacagatct ttctgcagca
    12541 aacctcaaat caccaaacta cttcatcaga acaagtattg atgaaaacat gtttcgttct
    12601 agtggagaca agatagtaga acaagtttta aacgttgaac taattagcat tgagacggga
    12661 gaggtatctt tcagtaacaa aaaaatctat cgcaaaaagg cagttagcaa tagaggagtt
    12721 ggctggtaaa atgagactct ttaaatacgt tagtttactc tgtttacttc tttttatatc
    12781 ctactgtgct tccaattatg tagaaaaaat ctctaaagca gaaaatgctt tttatgcaga
    12841 ggattacgat acagcgatcc cggctgttcg agatctagca aaagatgcaa gttcgaaaga
    12901 tgaattgctt tacttaatgg aagcagggac aatctttcat acaaaaggag actatgcaac
    12961 aagtaacaaa gtatttaagg atgcagagac gatagcagat caaattcaaa ctagtatttc
    13021 aaaagaaagt ttagcatttc ttttatctga caatgaaagc aatttcaaag gagagagttt
    13081 cgaaagagtt ttgataaaac tttatatggc attgaattat atgatgctaa gtgatattga
    13141 ttccgctaaa cgttatttca agaaggttaa ctttgaattg acggacatga agacaacaga
    13201 ctcaaaatac aagcaaaact tatttgcacg ttatgtagat gctgttgttt ccgaacagct
    13261 aactgcattt aatgacgcaa gagtcagtta caaaaacata atggatatcg cacctgacaa
    13321 taaagaaatt cttggtgata gatatgtgtt agcagtaaaa gagaaggata caagagatca
    13381 agcaaagtat gcagagggta aaaattatat caaagcatat gataagaact tgcagccgat
    13441 tccctacaat cctaatcttg cagaggtttt aattatcaat caagctggga aggctgcgac
    13501 caaagagtct aagggtaaac tagtcgatga gccgatgatt caacaagctc tttataaggc
    13561 tgtcgagtat acaatgcgaa gtatggatac aaagggagcg tcagttgcaa ccgttatggc
    13621 aatgatatca aacgcagaga atccgatccc tgaatataaa aaacgagatg agcaaaaatc
    13681 aaatccaatt gatatttatg ttaatggtat caatgtaacg aatactaaaa tatataatga
    13741 ttatactgat acggcaattg gaaacttcaa cgagaactat acgtctctag ttacaaagaa
    13801 cattgcatcg attgcaacaa aagttgtagc ggcaggtata gcagcgtatg ccgcaggtaa
    13861 agcagctgag aatgcagcaa agggcagaaa cagaaatgat ggaggcggag cagagataat
    13921 tggcttaatt attggggctg ccgctggagc cattgcaggt gcaggagtat cggctacaat
    13981 taaaccagat ttaagatgtt ggcgaacact tccttccaac ttccagattc gaaggatttt
    14041 cttagagccg gggacatatg attttgaatt caaatccaat acaagccgag cgatgggcgg
    14101 caagaatttt accaaaatat cggtggatgc cagtaaatta gtagtgatta acttccgttc
    14161 tctctagttt cttttagcta gcgatacaaa gaaggcataa tttttactat gcacgcaatt
    14221 cgtaaagaaa ttactgttat cctgtacgga gatttaaatg ttcaatagac taacagtatt
    14281 ctctttaatg gtttgtttga ctcttggccc aattttgcct caaacaaaag atgacacaaa
    14341 aatcgaatta aaattttcta tcgatactag atttggttta ggaaagggag tatttaaaaa
    14401 aacttctctc atggtaaaag acaaaaaggc agggacaggt ttagtagagg tggatgtagc
    14461 ttctattgac actggtttag caatgagaga caatcaccta agaggggaag atttctttga
    14521 tgtccaaaaa tatccaaagg cagaatttga aatactaggt attgaagaat caggtgatac
    14581 tgttaaaggg aagggaaatc taaccttaaa agggatcaca aaagaatatt cttttacggc
    14641 aaacatcata cgcacagccg atgtagaaaa atacacaggt tccatcaaaa tcaatcgaaa
    14701 agattatgga atggtttatc aaagtatgat gaatcctatt gatgacattg caaatcttga
    14761 gtttacagtt acactcactt ctaaaaagta gacttcatga aagcggttct aactctaatt
    14821 atagcaatct attcgggcat cctcctaagc ccaaaaatat ctctcaataa agaatctcta
    14881 gatttaaaca gttccaactt cattgcatcc aactccgatg atgaatatga tgacgaagaa
    14941 ggtagcgaag aagaacaaga gaacgaagaa atagaagaaa acgcttaatt atctatggat
    15001 gactccaata gttttttcgg aaaaattttt tcctttataa aatctctttc cgtagctcgt
    15061 gctgtcagct ttagctttgg attagcgatt gtatttggtt ctttaatttt atatttagta
    15121 gaagcaggaa atattagtta tataaatgca ctttatcttt ctgcttcttc tttctgtgta
    15181 accggtctta ctcctattcc aattagtaaa ctttcctttg caggacagtt tgttctcatg
    15241 atttttattc aagcgggagg attaggaatt atcgtatcta cagtccttgt tgggttaatg
    15301 gtaattcagg gtctttctag aaataccaaa ttacaagagt tcatgtttga agtattggat
    15361 acggatttaa aaaatgagga caaacaagat tctatcgaac agccaaaagt attgagggta
    15421 atcatcgcaa tttttaacat tgccattact attgaaatca ttggtgctat aattttatat
    15481 tttacttttc cagaaaattt gccgcctaac gttaattcac gagtattttt aagtttattt
    15541 acttcaattt cagcatttaa caatgcagga ttttctatct cagatgatat tagtttttta
    15601 atcaatgatc taaagagtct ttatgtaatt atctttttaa tcataatggg tggaataggc
    15661 tttccagtaa ttatttttat cgaaaaagta atgttacaaa cattaaatga aattacatca
    15721 aaatttgaag tttggtgtga aactcactta atgattaaag cgatcagtgg agaggaacct
    15781 tctccaattt atttcttctt aaccaaactt tctttttgga cagaaaatca aattgcaagc
    15841 tataataaat ctttacttgg ggaatcaaat cgagcacaaa ctgccattat tctcattggg
    15901 agtttaattt tattatcact tggcactttt tcttttttcc tcatagaata ctcaaatcct
    15961 gcaacgttag gcaatttatc tctttcgtcc aaatttgcta attcagtttt ggtatcggca
    16021 gcctctagaa ctgcgggatt taatacattt aatatgtctg gcatcttaga cccatctatt
    16081 gtaatcattt gcagtttgat gtttatcggg ggaggtcctc aaggaacggc aggcggtata
    16141 aagattacta cattcgtgat ttttttacaa taccttagaa atgtaatcaa ttcgcaaggt
    16201 aaagtagaaa tttacggaca ccttgtttca aaacgttccg ttgcgcaaag catacgactt
    16261 tattttcttg cgaccactgc tttagttgga gttgtatttt tattatcact aattgactct
    16321 agtcataaga ggctagacct aattgttttt gaggtaatct ctgcattttc tactgtggga
    16381 ttcacattag gcttaactga aagtatatct gatattgaaa agattattta ttcgatcgtt
    16441 atgtatgttg gtagaatcgg aatcttcaca ctccttgtta gtatcacagg gaattcagtc
    16501 acaagccaaa tgggagatca ggatgatggt ctcaaaattc aggtaggata aaaaacttaa
    16561 atatccagtt caatatccga tctaggatga gaattacatg tcgttatccg ctctctttga
    16621 ttttttgaga gagagctttg cgttgttcca tttgttttaa caacttggca tgatttacac
    16681 attcctctaa ggcaacctga tggaattttt attccgactg cttcgattgc tttaagtaag
    16741 cttgtgtttt catttaccac aacttctttg tttgatctca aaaacttaac tgtatgaaaa
    16801 acattttccc tagctggatc aaacacataa ctgtctggta gaaagttttc gtaaaataat
    16861 tttgattcag ggaattccaa ttctttcaat aaggtttttg ttttctcaat aaagcccttt
    16921 ggtgcacata aataaatctc tttatttgta agttcttcta tatcatcgaa gatttccttc
    16981 gaaatcgagc cagttaaaaa gccttcctta taatcatcaa aatataattt tgattttaaa
    17041 cctttcttct ccagttcata aatttccttt agaaataacg tcttctcata ggatcgatta
    17101 aaataaatta gaatgcatcc agaaagccta ttttcttttt ctagatttcg aagcatggat
    17161 aatattggag tcataccagc acctccagct ataaataact ttttataatt gctttttttt
    17221 agaaaaaaat cacctcgcgg tttccctaaa atagtaattt tatctttctt ctttagaaaa
    17281 tcatttatat aattcgaaac ttttccattt acaactctct ttaccgttat atgaaattgt
    17341 ttttttgaga aatcaggatt tgttaatgaa tattgacgaa tataacgttt gccatttatc
    17401 aaaatggaaa tagagatata agaacctgct tcccattcca cagatcccca ttcttttgga
    17461 gtttgaatta taatagattt ggcatcccca ccaatttctg attcaacatc aattacttca
    17521 gcctcagaaa tggaaaaaga ttgttttgtg ctaaatgtat tgcctatatt gaaaaatccg
    17581 ctgagtattc gattcgtcaa ttcaattgga ttcaataaga gtttccaaat atttccatta
    17641 tcttctggtt ggataggtag agatctgagt aatgcatttt cgataacccc cataaactgc
    17701 tcccagaatg atccagttcg ataaggaact ccatattttt cgcataacgc tttcacttta
    17761 ggtgctactt ctcttaaacg attcggagga agatctggat aaagatgatg ttcaatgtga
    17821 agatttattg ctccatagac tatttcgtaa taaggtgggg aatcaaaatt aacggtagag
    17881 agtatttgcc ttaggtaaaa ttctcctttc gactcttgat ctttctcctc aaaaacatag
    17941 gcatctttat gaagatgtga aattgcgaga atcatttcta aatatatatt ctgaaacaat
    18001 ttagcaaaga aattacccgc tgcaatttta aaataatttc ctccaggaag catcggaaaa
    18061 aaaatataat tttttacaat atgtttggaa accttctgaa tagaaggtag ataagtttcc
    18121 agtgttaaaa catctctcga ttcggctaac gctctagaag tagcaacata ccaaggaaag
    18181 aaaaatgaat taatcagagg catggacaaa agcattggaa cctgaaataa atggtagagt
    18241 ttccaaggtt gattgtcgtt tacacggtag actaaaaatc cgaagtcatt gtcttttcca
    18301 agaatgttag taaaaggatg atggactcta tgatgttcga acttccaatc ttcttcatcc
    18361 atagtaatat cccatttcca gttgcttgag agaacagtat tgttattttt tatatcatca
    18421 tattgaccat gcaaaatatt gtggcctaac tcactaaact caattagaaa atggtaacct
    18481 aaaagtaacg acccacttgt ccaaagcaaa ggctcttggc ttatatgaat caaacttctt
    18541 cccacaattt ctaaagcatc agaaaaccgt tttacattta ggatgtaatc taagtcctca
    18601 tgaccaattt tcgattttgc ttcttggtat atttcttcaa tttccttacc aaaactctct
    18661 atctgctcat ttgttaaatc tagtttgtta ttcataatga catccaatgt agacaacatc
    18721 tacttcagga ggaatcttgt caagttttaa agtagacaac gactacatta aatttatttt
    18781 tggatatatg aaaaagaaga agaattatca tcatggaaac cttaaaagcg cttgtatgga
    18841 agaaggctta cgtttaatcc agaaaaacgg ttcttatgat ttcagtatga gagatatatc
    18901 caagaactta ggagtttctc atggagctcc ctataaacat tttgaaacaa aagaagctct
    18961 acttgcatct cttgcggagg caggatttca caaactcaca aatcaattgg aagaagtcaa
    19021 aaatgaaaat tataaaaaca aactagagct actttataaa atagcagtaa tgtatattca
    19081 attcggaact acaaaccctg aatactacaa actgatgtat ggcggaatca tacaagatgc
    19141 agaaatgtat ccgagtttat atgaagcaag tgtaaaatgt tataacactt ttttagaaac
    19201 aattgaaagt ttaatgaaat caaaagaatt aattccatcc tctgattcaa accttttaac
    19261 cttctacagt tggtcgctat tacatggatt agtctcactc gcacaagata atagattaaa
    19321 tattgctttt agagaggcta atgcaaataa tccgcaacaa cttatggaag aacttttttc
    19381 ttatttctta aatggggttg gccataatct aaaatagaat aatttatttt gctaatggga
    19441 cagtaaagaa gaatcgactc cctctattta gttcgctctc tactccaata actccattat
    19501 tcttttcaat aaactcttta ctaaatagaa gtccaagtcc agttccaagt tcaccggatg
    19561 tgccgattgt tgaggtagct tcgatgctaa atattttttt caaattttca gaagaaatgc
    19621 caaatccagt atctattaca gaaatttcaa cattgtcctc tttctccagt aaattcactt
    19681 ccactctatc cccttcttta caaaacttta ttgcattcga aagtaaattt cttactacca
    19741 attgaatcat atttttatct gcataaactt tgaagctttt tggtaagtta tgatttaggt
    19801 ttattttttt attcttcgct cgtagttcta ccaaagctat ttcatattcc aacaccttgg
    19861 aaatatcaaa tgtagttaaa tgaactttag ctccccccat ttggcttttt gcccatccaa
    19921 atagactatc aactaattct agaatcttat ttgcgttatt cgttatatca ggaatactat
    19981 ctgataattc ctctatcgat aatactccat ctctaacaga gttcaaggtc aataataagg
    20041 atgtcagcgg acttcgtata tcatgagcaa taattgaaaa taatttatct ttaatacgat
    20101 ttaattctgc taattgttct gattgaattt gtatttctga tttttgtttt tgtaattctt
    20161 cattcttttc agatatgctt ttaataagag actcaagctt cgcttgaacc tgtttcgttt
    20221 ctgtcagatc catattgctt cctgatacac gaacaggtgt ccctgtatca tctctttgaa
    20281 tatatcccct agacaagatt ggtatatata ctccactctt accgagcaaa cgaaattcaa
    20341 gttcatagct tgttatgttc gattgaatta attcctgtgt tttgacctct atacgttttc
    20401 gatcttcagg atgcatcaat cgtagccata aagatgaatc tgattctatt tcattgactt
    20461 cgtaacctag catattccac caaagcggcg aataatacag attatccgta ataaaatccc
    20521 agtcccacca accgttattt gttcctttca aaactaaatt cagtctatcc tcagattgta
    20581 ttaatatagt ttctgcatac ttaaaatctt ttttatagat atgaataatt gtatataaaa
    20641 atgcggtagt agcaaaagag ttaataattg atcctaagaa aaatgtatcg ggatttatac
    20701 ttaaatattt cactttcaaa aaggcaataa tcaaatacga aagtaaatta tagaacaagg
    20761 aaataaaaac ccaaccagta ttaaaaataa aggcaccaaa aaaaccgcag cctaaaatca
    20821 atacttcaaa attaacattt cttccattaa ataccccaaa cactgcatac attaatatta
    20881 gtgtattgga agcaaagaaa gaatagattt gagcaatctt attgcgatcg atgtaattaa
    20941 accaaatgat tggaaccgaa ataataaaaa taactgaaat gttcaatcca agtggaatga
    21001 atctttgtgc aattagatta aatagaaata gagtagcatt aatgatacct aagaaaaacg
    21061 aaatttgatt taaggttttg atttgtcttt tttcaaatac agtatgacta ttccgcatac
    21121 ctaaggaaca tattctatca atcacttctg taatgtaact tttcatttgg atactactct
    21181 gaaccaccat aactcagaaa gattatatta caaataaaaa atcatagtaa tcccactttt
    21241 aacatttgtt atccgctaca ttgtttcgat cctcaattca gaattgagaa aaaaaagatt
    21301 taaccttcta gtaaaggaaa aggatttttt tattttaaag tgcgcttaac acacattctt
    21361 ggtgtatgaa agatatattc attgggttga aacttttttt ataagtctga atagagtcat
    21421 tttattcact caccttacat tatcattgct tcataaaagc aataattgag ttcagttgag
    21481 ccaaacgttc atatgaggtt tgctcctaac agagagcttg ccgatctagg cttttgaatt
    21541 tataaattaa ttggcttatt ttaagtttat aaaaataact ttctttttaa attttccttt
    21601 ttgtctaaaa tcaataatta agtatttact tccctacttt gttctattca aaatccttgc
    21661 cgacaagaag aaacttttaa tagaaactat actaaatttg tttcataaag gaaaacatgt
    21721 gcttttttaa aaatgaatac cactcgcaaa ataaagtcgt aagaattctt tgtttgtagg
    21781 atccactcta agttcatcga aaataatttt tctttctgtt acaggattaa cgtgcgcgat
    21841 acacaaagat ccataaaaaa atcctgaaaa cggatctgta ttggaattgc gatacattcc
    21901 tgaaaaaatg acttcattcc ccactcctaa atttgaattt gtatgattca aaatggtaga
    21961 gacctgaaaa gaagcactca gtggatagct aaaaccaaga ctaaaaagtt gcattccttt
    22021 tgtatcatat cgattttcga tagaagaatc aaaatctcta aacaatgttc catttttcgc
    22081 attcacattt tccattaaaa gaaaagaaga tttcccccca aagattctta catcagatag
    22141 tagcgaagaa taaccgttat gcgctctatc tacagcattt gatttatcct ttgtagaata
    22201 aagacctccc aatcttaaat tgagttgatt tagattccaa ttaaaagcaa gataagacaa
    22261 aaatgcattg gttttactaa tatggttcaa ttgttgatag ctatactcag cataatctct
    22321 ataacctttt acaaaaaata atcccagttc tgtatcaatc ttccagaacg gatttgtttt
    22381 taattctaaa ccattgtaga taaatcgtcc atttggttta aactgcttat ctgcttgata
    22441 atattgtttc tctgcgattt gacctggctc tacaacaaga taattaaata tttgtaaatg
    22501 atttatgaat ggaagagagg taaacagtaa gtttccacca aatattttat cattggttgc
    22561 attggcacga attgtaaaat tttcttgttt gtagttatag gaaagagcca agagagaaaa
    22621 cttaattcca aatggtttcc aaaataaatt agattcaaag taatttgtaa ttccattaaa
    22681 aaaaagtcca taggcatcta gtctttgaaa tcctcgtcct ccttctatgt ttatcgaaaa
    22741 atttgattta tcaaatttcc atgcaagtat tgcctctgta tcgaccactc tatctacact
    22801 tctttttaaa tgataatttt ctttgtagga aagaatagga gaagccaaaa tcataaactg
    22861 gttattacct gagcgtttaa aaccaacaaa ctcaggtgca aaatttgcct gtgtcgcata
    22921 tcgacgcgct aagtcttctt tgtctcctcc tttctgagag tagaacttag aagttataaa
    22981 agaattgtaa ataaacttag tatccgatcc ttcttctcca gtatccttgt ctgtgttttt
    23041 agacttagca cttgtttcga ttcgtccttc gttctgaatt ctctttctcc cattatttaa
    23101 ataatttaaa tcatccaaac gatagtctct gtagctttca ctgaatagct ggaaagttat
    23161 aaatataaaa ataaaaagaa attttttttt tgtattcatt cgactattga tataaaattt
    23221 atactactac acgaaatatt tattcgatta cattgttctg ttttaatcat tgtattcaat
    23281 tgatctctca ctatattttt actccaactc aaatcattac ttcctaaaac tgaatattcg
    23341 taattgccaa atgaaagaaa agattctatg gtataaaaat agattaaccc ataagattta
    23401 gttattcttt tttttagctg tgaggaagtt agtaaaaaat ccttctctac tttatgattc
    23461 ttaaaaataa aaattgaaaa atatttataa tttccctttg tataccagga aaaagttata
    23521 tcctcttcaa taaaaaaatt ataattatat ttaaaaggat aaatagcctc taaggtggaa
    23581 gaaatcggtt cttgtgaaat atcatcgttt ttatacgaat cttgcgcgac gtaatctttc
    23641 ttatccccag ttgatgtatt gataaaagca acacttgcga ggataatcgc gattatgtgc
    23701 aaaaatttat tttttcgatt tggttcactt tgctctctag aatatttttt ataaaaaaaa
    23761 taattcacaa aactacaaag aggaatacag agcaaatcac tcttatccgc aaaaccattt
    23821 tgatttccaa agcagtttgc atagatccaa ttattccatt cttgatccca attgataata
    23881 aaaaaaagaa gaatgatact taggttcgca aagaaaaata aaatcttttc caactctttt
    23941 tttcgaatca ggattgaggc taaagcagtt aataaaaaag gaaaaacaat gagaccagtt
    24001 atgtcgctta ttttaccagt aattgtgtta tgaaattccc atttcccaat atgatcattc
    24061 caaatccata ggcaaatcaa taaaaacgaa actggagagg aaagaaattt acccgaattg
    24121 gaaagtgttt tcaacattca gttgaatctt cttattctgt ttaaaaataa agcaatcaaa
    24181 aaccaatcct tttcaagaaa gaatctgttg ggtattaatt atttcctaga caattctttc
    24241 aggaatttta acctgtgcta gaattctatt ctggagttat atggatgaaa aaactattac
    24301 tttttgtcat tttgacaaat atgatttcct gctacactta cgtaaatgat gagttaccaa
    24361 gaatcagagc aattaaacca aaaattaaaa ttcctgctat ttcttttcga atcactggaa
    24421 ttgatgataa agacagctta aaagaataca acagaatcat aagaaaaaag ctagaaaatt
    24481 caaatctgtt caatgttgta aatgttattg atgtgaatga caaactagat aaagcggatg
    24541 ctcatcacct tgagattcac ttaaagaagc aagatgtatt tgaaaaccga tggttcctag
    24601 gcgcatctgc ctttgtatct ttatggactg ttactcttgt tccagctttt gagaaaaaca
    24661 aagtagctat gttagttgat tattatgtag atggtaaact aaaaagatat gatagatttt
    24721 atcagagctc aactcgactt tttggtatca ttccagcagt gtttagagtt agaaatggag
    24781 attccgttga aaatccagat gctattatcg tgaacaattt agctgataat gcactttatt
    24841 atttaaacga attaaacttc tccgaatccg ttcaatagat ttaatccagt ccatgggatt
    24901 gtgtgaaaca aatctaaagc aaaggatagc tttaacacaa tctcaaattc ctgaatcgct
    24961 gagtgtaatt actcagcgtt ttgcgtgaat aaataaatac tatgattgac aatatagatt
    25021 atagcaaaaa ttctcaatag tggcatgtct tcagaaacac gaaaaacctc agaaacagat
    25081 attaaactaa aactcaatat tcgcggaaaa ggagagtatc aatttgatac agaaatacct
    25141 tttttcgaac atatgctctc tcatattgca aaacacggtc acattgatat ggatctttgg
    25201 cttaggggag acatcgggat tgattgtcat cacagcgttg aggatacggc aatcctactc
    25261 ggagcaacac ttcataagca attaggggac aaaaaaggta tcaatcgata cggacatttt
    25321 accatcccca tggatgaagt tttaacaacg gttgcagtcg acttgggtgg tagattcttc
    25381 tttcaatata caggacctaa attagaaggt aagttcggaa tctatgatgc agaactttca
    25441 cttgagttcc tacaaaaatt cgcgctgaat gcaaaaatga atcttcatgt tatagtccat
    25501 tatggagaaa atagacacca tattcacgaa tctattttca aaggacttgg aagagcattg
    25561 agacaagcat tagcctttga tgatgatagc ctcggggtga ttccatcgac taaaggaatg
    25621 ttagaatgat agcagtaatt gattttggaa tgggtaatat tcattcctgc ctaaaggcta
    25681 ttagtctatt tacaaataat tttaaactaa cgaccgaccc taaagattta gagaaagcaa
    25741 gtggcattgt tttgcctggc gatggagctt ttgagaaggc aatgtctaat ttaagagaac
    25801 ttaagtttat tgatcctttg cataacgccc ttgaaagaaa aattccgctt tttggaattt
    25861 gtattggatt tcaaatttta ttcgaggatt cagaagagtc cacagaaaaa ggcaaactag
    25921 taaaaggctt aaatcttata aaaggtcatg taaagaaatt ctcattaaga aattataaag
    25981 tgccacatat gggatggaat aagttaatta taaatacaaa aaagaaatcc attcttctaa
    26041 atgaagttca aaatggagag tatatgtatt tcatacattc ctatcgtcct gtcgatgcag
    26101 atcaaacatc tataacggct aattgtaatt atgcggaaga gaaatttcca gtcatagtag
    26161 aaaaagaaaa tatctttgga actcaatttc atccggaaaa gtcggataaa gagggactca
    26221 aaattttaag aaatttcatt cgtttagcaa attcagaata aggttttatt atatgcttat
    26281 tatacctgca atagatattt tagataaaga agcagtgagg ctctacaaag gagattactc
    26341 acaaaaaaca gtttatagtt ctaggccatg ggaacttgtc caaggcttca ataagaatgg
    26401 tgctgagtta attcatttag ttgatctcaa tggtgccaga agtgcggatt ctacaaataa
    26461 ggattgtatt atacgaattc gcaaagaagc gaaggtcaga atccaattag gtgggggaat
    26521 tcgagacatg gaaaagttaa aatactacga atctcttgga attgataaat tcatcatagg
    26581 aacgtcagcc gtaacagatc caaaatttgt agatgaagca atagcctttc ttggaacaga
    26641 tcgaattata attgctgtcg atgctttaga tggatttgta agagtcagtg gctgggaagt
    26701 aaaaaccaaa gtaaggtatg aagaattatt aacaagatta aactctcagg gagttactca
    26761 aattatattt accgatattt cgcatgacgg aacaatggat ggaccaaact taaattctta
    26821 taaatatata ttagataact ttgactttca gctaattgca tccggtggga tagcctccct
    26881 aaaagatatt atagatctaa cagatattga tgctaaaaga ccattgtatg gaatcattac
    26941 tggtaaggct atctatgaag gaaaattaga tctcaaagaa gctattgata atactagaaa
    27001 gtaaaaaaga cacacttgaa attatttaaa aaagggtaaa tgaagtaaat atgaatactg
    27061 acaatttaaa aaaatacaat ctaataggaa taattgttac aataatcgga ttgtctctct
    27121 gtataatgct gatccaagaa ttttttggat ctgcgggcgc atttgctgaa tcactttgtt
    27181 cagcaacggg tgatggggac tcatgtagca aagttgcaaa gagcacttgg tcaggtttta
    27241 gaggtgttcc aatcctagga gatattgctg tagcgcatat cggttttact ttctacggtt
    27301 tatgcggttt cctgtttttt tctattttaa aatcaaacga agaagataag aagggttttg
    27361 taagtgtatt atttatattg tctattatgg caattttaat tgatgcaata ctattactga
    27421 tctcagtaac tcaaattaaa actgtttgta tactctgcgc acttacctat ggagtaacgt
    27481 taagtatatt tgcactttct tatatccaaa tcaaatcatt atcgggacag gaaggtatta
    27541 tgaatcaagt taaagcaaat gttggaaaaa acataacagg aaatatctta aactatacaa
    27601 tagcagtttt attgttttat gcttgtggaa ttggcgtatc aagactttca aattctggta
    27661 gtaataaacc agaagttgac cctgagtcaa acaaacaata tttacaaagc aagatagacg
    27721 cttttgaaaa aagaagtcct gttacaatca atctaagcca agcacctaca atcggaaatc
    27781 caaatgctcg agttacaatt gtgaaatatg ctgatttcaa ttgtggacat tgtatgcaca
    27841 cgagccatat cttacgtcaa cttttggcag agtttcccga ccaaattaaa atttattata
    27901 agaattttcc tttagatgga acctgtaatc cactagtgca aagaaaagac ccaagtgcaa
    27961 gttcttgtat cgctgcgagt gccgcacttt gtgccaacaa acagggcaag tttaaagaag
    28021 tatataacgg tctctatact gataccgaaa atggaattcg tcataccatt agctctgttc
    28081 gtgaaattgc tacaaagtct ggagttaacg ttcaacaatt acaaacttgc atgggatcaa
    28141 cagaaattca gaattttatt ctacaagaag tcaaagaagg agaagtttta aatattcaaa
    28201 gcactccttc tctttatgtt aataataaac ccttggatcc tggaacacca aatccaaatt
    28261 tcttaaaagc gttagtagaa cacttattag ctaaaaagta atctatctca tcaaaaagtt
    28321 gcaataagaa aacttgcaac tttttgattc tttaaaaccc cacctatacc aaacatctat
    28381 tgtaatctct cttttttaaa tccaaaagaa cggttcggtc taaacacctc tttgtgttcc
    28441 cgcataccat ggtagagcaa actcgatcgg aagtcattac tgagtattta tttttcacaa
    28501 agatagcttg tagcaataag aattctggtt ggttgcaaaa taaaagcaga atagtgattt
    28561 tgggataaaa ttgataaaac gaatcctcta ccaaactaat attgcaagct aatcaaaaat
    28621 aattagaatt gattactcaa atcagaaaga tgactgttta aaaaactgaa ccacatactc
    28681 attcagttgt tcgaaagaag aagctttgaa gacctctaaa tactctctag atgtgttatg
    28741 atccggtgat ccctttaaat aataagcatc ataatggtgt tttaataata gataagctgc
    28801 cgtatatcga tcatttgcta cgattgcttc agcgattgct ttaaaaatct ttccatctat
    28861 aaattgcttt aaaaacttaa tcttatcaag aatataatca acaggaaaat tatattcttc
    28921 tgccaatcgg tttgctcgaa cttctagagg caattcgacc caaatctttt ttcctgaagt
    28981 catttttgaa taaaatctgt cagggattag aagacgtcca atttttttac tctctccttc
    29041 tgtgattaca taggaggact gtatcttaga taaagcatgg aacaaaagag attcgaacat
    29101 tttttgtgaa ggttgattta tcatatctcc tcccaaaagg gaacctttat gttttgcgta
    29161 tttttccaag tgaactgctg gtagttggaa ttttatcatt ctttcaatta actctgattt
    29221 accgctacca cttggaccat aaatacaaac agtatctaat tgttcagtct catgaaaaaa
    29281 gaaattcaca cgtcttctat aggctttgta tcctccatca actagcgttg ttttatatcc
    29341 tattaaatcc atcactgtaa ataaagacct agaacgcata cctcctctcc aacaatagac
    29401 cacaaaatga cagaagggtt tatgcttatc atcagatccc ttattaaata cagagatcag
    29461 ctcttcaact ttttccaaat ttccttttaa attttgagca atcaactttg cacctaattg
    29521 tcttgcagta aaaggattct ttttgtattc ggttcctacg atttcacgtt ctttatcatt
    29581 taggactggt aaattaattg ctccaggcac atgatcttcg gcaaactctg attcacttct
    29641 cacatcaatt aggatatgag agttactaga atattcattt aatatctctt cgattgtttt
    29701 tgttttagat aaatgtatca tagaagtaac aataaaatta gattgaaaat ctatacgata
    29761 gagttaaagc tatttccatt aattatgatt aagtttaaaa aatacataat tttttttacg
    29821 attcttctct ttttatttat acctaagaat attttctcat ttatggatta tccgtatgga
    29881 gttggatttc agatgataca aatccctctt ctacctatcg acaatgtaaa tcctggacag
    29941 ggtaaatttt caatgagagt aagtggtcgg ttgatgaatg ctttctcttt gcaaacaaac
    30001 cgctttatta tagatgggga agaaggccaa atagagcctt ctttacgtta tgcgttatca
    30061 gattatactc aaataggttt cagtcttcca ttcatggcaa ggggcggtgg ttttttagat
    30121 catagtatcg aaacatttca caggataaca ggcgttactc aaggaggtag agatcactat
    30181 cctagaaatc aattcaatat cagttatgag ccattagcac aatactatcc actttttgac
    30241 tcaactccac tgcaagctta tttaaatcga aattttgatc aaagagaata tcctagaaat
    30301 tccaatgacc aaccaattca attttctaaa caaaatgatc taatacacaa tttagtgatt
    30361 aacaagtatt atccatattt aaatgaatat agaacagaag attatataaa taacttcaac
    30421 tcggttgcta gaggaaatcc aaagttgttt tttcagacta cagtattaaa aggaagcttt
    30481 tggtttgata aaatcacaat cggaaatcaa gtcagatttg cgaccaaaac tagtgaatta
    30541 attggaactc ccggaaatga tgtttcaatc tttggaatct atcacaagga ttggtttgat
    30601 ggtagagtaa attggaagtt aggtgtatcc tataccagat ttgaatttgc aaagtatcga
    30661 aatttagatc ttagaaagga tgaatggaca atacgcccct ctttaaccta taacatggat
    30721 gaaaattgga agcttcattt tgaatatgta tattttcctt ccactatact tcgattcggg
    30781 agactttcag aacctgcaca tcaaatagga attgggacaa gttataaata taaagactac
    30841 caattgcaat tcgccttatt tgaaaatcta ataacatatt caattacacc agatattgga
    30901 tttctgttat caatagaaaa aactactttc aattaagata taagatagac cttaagtaaa
    30961 acgttccaaa aagctccatc cgtccctaga tgtatagatt aaaaataata tccatcttct
    31021 tattcgatat tttttgaaaa aatcagaact tattcattca acccatccat tagaaatttc
    31081 taatatattt tttagccaat agattactag attagaaaac ggaagactat acaaaattat
    31141 ttacatttca aaatgatatt atttttatta gagaaagtat tagaatagga tagacatatt
    31201 ctaatttatt gaaactatta tatttctatg agagcatttc aatttatttt cctattactg
    31261 tttgtatttc caatctttgg aaattcattt aaaattgatc taacacaaat ttgccaatca
    31321 ggaaaatgcg atcctcgtat atggtattct accgattctt tcgaaaataa ctttgtaaaa
    31381 ctagaaacac ctccagcgga ttggaaacaa gcagacaaat ttccgatctg gatgaataag
    31441 cattacacaa aagaaggatc attggcaacg tattcattac taactttttt cgacttaccg
    31501 gataacgtta cacttaaccg tcaaacagga attcgattgg gagaaatagg agaagtcttt
    31561 gaaatttata taaacggaaa tctaatcatc caagaaggta aagtaaatga ccacgaagtt
    31621 atttttcaca gaactgtaag aggaaagatt tacgaaatcg ataaaacaat tttaaagaat
    31681 aaaaacaatg ttctgcttat taaactttca ggtcatccaa agtatgacca tactggtttc
    31741 tatttaacaa agggctatga aattggtttc tttgaagact tgcaatatga tgagcaggat
    31801 cgaattacct taatactgat tggaatttat attacgatag gagtttatca tctcttttta
    31861 tatacaaagc gaagaaaaga tttttacaat ctatactatg ctctttacac tatcggactt
    31921 ggaatatata tttatacacg aacttcagca attttcgaac atccatggga taccacactt
    31981 atacaacgag tagaactgat tgtattatat cctgttatat tttttaattt tgcattacaa
    32041 gaggaattgt tttttcaaag aattagaaag gtaactaaat actatgggta ttttttgttt
    32101 ttatggtcat tcataacaat ttttattcca atgtatatcg ctgaatattt actccgattc
    32161 tggcaactaa cttttattac tttaggtatt ggaatcgcgg ttaatattat atatacctct
    32221 gttaagaata aaataccaaa tgcaaaacgt ttaatgaccg gaatgatcat tctgacattt
    32281 tcagcagtct ttgatgtaat ggactctctc gttttaaata cagggctcgc tttcagtaag
    32341 tatgctttct ttatttatgt ttttggattt gctacaattc tagcaaataa tttcattcaa
    32401 gttcacaatg aaattgaaca tttgaacgac accctcgaga gaaaagtcat tgagagaacg
    32461 aaagagctaa cgaatagctt agaaaaagtg aaccaactaa aaaagcagca agatggtgat
    32521 tactatttaa cgtctctcct tttaaaccca cttggagtaa atcatgcaat taaaaaaggc
    32581 gttgatatag aatttattat acagcaaaaa aagaaattca cattcaaagg aaatgattct
    32641 gagttaggtg gagatttatg cagaacggaa actatttcct taaaaaatcg tcaattcaca
    32701 gtttttttta atgcagatgc aatgggaaag tctatgcaag gagctggtgg agcgattgta
    32761 gttggcgcag tatttgactc aatcattgaa agaactaaac tcagtaaggt tgttcaaaat
    32821 tatcatccag aacgatggct gaaaaactct tttgttgaac tacataaagt ttttgaaagt
    32881 tttgactgca caatgcttgt ctccattgtt gtaggattag tagataacgc atcaggatta
    32941 atctactaca taaatgcaga acatcctttt cctgttttgt atcgggacgg caaagcctca
    33001 tttattggtg atgattttat ttatcgcaag ctgggaacac cggtaaatga aaccgcatat
    33061 atctctgttg atacatttca actaaaacat ggcgatatat ttatctctgg gtcggatgga
    33121 cgtgatgata ttattataaa aaattcatta ggtgaaaaag ttctaaactc tgacgaaaag
    33181 ttctttttaa gagtagtaga agatgcgaat ggagatttaa aaactatcct tcaaattctt
    33241 aagcgggaag atgaattaac agatgatatt tcattattaa gaatatatat aacagaaaat
    33301 ccctatccat tcgacatgtc acatttagaa gatataatta accaaaagag tataatagat
    33361 attgaggata ataaagattt accgcccaaa gagttgtata ttcgaaattt aaagaataaa
    33421 ttctttataa atataaaaac caataaatat gaagaagcga ttcaagctgg aaaagaatat
    33481 ttagaatgga tccctgcaga tgatgaaatg atttctaaat tatccgaaac ttttaagaat
    33541 gcaggaagaa tagatgaatc aatcgaatta ggtgaacgat taagacttag gagaaagcaa
    33601 aatattccaa acttacttaa tttagcagaa tgttacagaa tgcaaaatca aatagaaaga
    33661 gcaaacttac tgcttaaaga tgttctcgca tttgacaaag aaaataaaat tgcgaaagat
    33721 atgattagca agttagttta aaatatgcgt tatacataat tcctttagtt ttgcttgtgg
    33781 aagggttgca tctaaccgca atgcgtcagg cggaataact ttttcataag caattctaac
    33841 ttttcttaat gtttcaagat tctcgaagtg ctcattcgca ttccttcgtc tggtcctagc
    33901 taaagatatc tcaggattaa tatctaagta gataattaga tcaggctttg gataatttct
    33961 ttttgtattc ataagtagaa tttcttcagg actgatttct gaatctgatt gataagcagc
    34021 cattgaataa aaatatcgat caagtattac actggttcct gctctcaggt ttggatatac
    34081 atttctttcc aatgaatcat ttcgatctgc taggaataat tctatctgct cctctcgtga
    34141 taatgaaatt tcctttttga gaaattgtct gatttttatt ccagtttcaa attgggttgg
    34201 ttcgttaaat tttatcgttg gaatttttct ttctaacaga gttgataaga ccatagacga
    34261 aagagtgctc ttgccactcc catctattcc ctcaaatact ataaacttag gtttcatcat
    34321 attttattag cttacattaa aaaaaagatt cgtattctgc caattgaatt tctgatcaac
    34381 tacctagatt ctattcaact cgattcaaat ggataaaaaa taattcaatg atttgtattt
    34441 tttatttttt tgatacacaa ggttaggttc ttgtatagaa tcgatatttg agaaaatata
    34501 tatggcaagt gtagaaccaa acgatttaat ctccatcctg aaagaaaaga gagttgatga
    34561 gtatagaatt attgaacaaa agaaaatgat ttcccaaatc tcagaagaag agattcaatc
    34621 aaagtatagc aatgttttta cttctataca ggatattgac gatattttac tcagaaaagg
    34681 agtctatttt gcactgcttg gtgcgatata cgcttttcac gaccgagagc taaatccact
    34741 agaagcacaa cattttccat tattccaagt gattaaaaat gatattttaa aagtttataa
    34801 cgaaatttcc gattcacttg ttgatgaaga gacttggtta tttgaaacct ttgactacgg
    34861 aatcaagcat gtctattatc tagattggca gttgtatctc tcgaagattt gctattagtc
    34921 aggtaaagtt aaaatctcat accctttatc agttaccaaa acagtatgtt cgaactgtgc
    34981 agaccatttt ccatctttcg ttctgactgt ccatttatct gattttgaaa ctacaacatg
    35041 atagcttcct agattgacca tgggttcaat tgtaaatgtc attcctggtt ctaattttgt
    35101 tagaagtcta gtttgtttaa aatggggaat ctgtggatct tcatgaaatt tttttcctat
    35161 tccatgaccc attaaatctc tcacgattcc ataaccattt gcagttagaa attcgtctat
    35221 cgcattagaa atatcgttta ctcgattacc tggcttgact tgttcaatgc caatccacat
    35281 tgctttttca gtatcagaaa caagtttctt tatttctggt ttaacttttc ctaccataaa
    35341 ggtttttgat gtatctccat ggtatccttt taaaattgga gttacatcaa tgttaataat
    35401 atctccatct tttaatatat ctgttttttt gggaatacca tgacaaacaa catcattaat
    35461 tgatgtgcaa atcgatttag gaaagccttt gtaatttaag ggagcggata ctgctcctcg
    35521 tttgattgta tattcatgac aaatatcatt taactccaat gtgctaacac ctggtttcac
    35581 aaagggctca atgtattgca atatttccgc ggcaagtttt cctgccgctc tcattaattc
    35641 tatttcagct ttatttttta tgtgaaccaa tttctattcc aactaaaaat ctgatgctct
    35701 aactgtagat ctcttattgg atttagttct ttccattgct tcatcgatta atcgataaac
    35761 tttttcattt agaccttcaa tcacatcact agatgtcatg agctccttag acttgatata
    35821 ttcttttact ttactggaaa caattaatat tgatttttct tcagacataa aatcctcttt
    35881 ttttttatct atctatcaaa taaaatgtag actcattttt agtaaattaa gaaaaactcc
    35941 aattctattt tttaaaaaat aattcctata ctctagaaaa atactcaaca ctacataata
    36001 ttttctttac aattttttat tttatgatat aattttactt ccaataaata tatatagaac
    36061 aatctaaaaa gaggagagat aaacaagaat ggaactaact acaaaatttt ttaattttat
    36121 tctaggccta agtggacagg ccatggaaga agcagataga attaaacgtt cattagaaaa
    36181 agaatattca agaattctct caatcggagc aaataataac tctggtccag cgattgccct
    36241 tcgagaaact acagaaacag taattatttt ttttgaagat ttaaaattca aattaaacct
    36301 gtaaaactag caagagtttg aatactactc aaactctaaa caaatcacaa ttaaaaatcc
    36361 tagctaaaaa ctaataagag ttaccattct caagatgtta gctcttatta tttattattg
    36421 cgtctactag tgatttttct aggtttaatt tctgtgcaat tgtatcagaa gtccaatcaa
    36481 acttttcata gagactcaga actgtcgctt tcttacgttt gagaatttca gtcgtatctt
    36541 tgtctaattt aaaattctta gagaagttct ttcccgctga agttgtagta acagcatcga
    36601 cgacaactaa aaagtctgaa agtttatcaa acttatcatc ggcttcagct agaagatttt
    36661 caaagccacc tttcatttct tctgtttcca tctttagagt ttcaattctc ttctgcatgg
    36721 ttgcaatttg tttatggcga gcagataagt ctaccattag attttctact ttatcgaatt
    36781 tagattcaat agatttaata tctaagttcc tagcactaag agattgaatc ttctcttcca
    36841 tatgattgat tccatcttct agttctgatt cacgagactc aattttctct actgctctca
    36901 ttactttttg aactcgatct gtgatttcaa atgtagattt attagaatta ttgagagatt
    36961 gagttaaatc attaattctt tcatttacaa tcttcacttc agaaagctta aattctaatt
    37021 cattttgaat tcgctcgatc tcaattaact tttggtctac agatgtaatc atggatattt
    37081 tatcatgaag ctcatctgtt ctttgattta tcaaatcaca tacgttagta gcacgagcta
    37141 attcattttc tatctgatca attctaagtt tatgagtttc gagtaaacgc aactctgctt
    37201 ccatttcctt tttagtagct ttaattaatt ctgcatgttt taagaattga tttgcagttc
    37261 caccttcttg cttcaattgc tcaagcattg atcctagatc tctcattgtc tgttcagaat
    37321 cttttatcat ttgacgtgtc ttatcaaata gtcccgaata cgattgaatt tcgtctactt
    37381 ttgatgtaat cttatccaat gtgtcacgta aatgatttgt ctccgcttcc acatattcag
    37441 aaagaccttt cttaatatca gctactttgt ctagatcagt atctatgcta tttttaatac
    37501 gcacgaactc ttctctagtt ttagagaatt catctctatt tgtctccaag aatgcgttat
    37561 ttgctttctc gatatctcta aatgttttct gaatttgaga gttgaaagaa gaaatttgtc
    37621 tatcactgtc ttcttttgca tttttaagag tttgttcaat attttgcttc acatcttgaa
    37681 cataacttgt aattctagat tcaagtttgc caagttggag ttgtccttta tctaatagga
    37741 cagtaagttg acgtgaaatt ctctcatcaa ttgtttgagt gagtttatca atcttctctt
    37801 cttgtttttc gagaagattc attcccgctt tctgaatatc ttctaataat tcttctgact
    37861 catagcgaac tttttcgagt ttgttttgta gatctctata gagtttttca ccgtagtcag
    37921 caagaagaga ttcttgtttt tcataaagat tgagtccagc tttttggatt cttccaagca
    37981 cagaatctac ttcatcttgt gattttttca ctttgccttg aacttctgtg ataataccat
    38041 ctctttgagc agttaacgct tctgtatgcg tcttaagcaa tgcatctaac tgagccttac
    38101 ctgtgttaaa ctcattttta aatatatcag ctaattctct accttcaatg tgaatgtctt
    38161 ttaatctttc aatcagacta tccatatctc tctgagttct agatttaatt ttttctagtt
    38221 ctaagttcca ctcttcaaca aagtgctcga tttttccatc ggtcttcatt ttttctttat
    38281 ctaagatttg aactacactg tttacttctg tttctacttg ttttacaatg tcttttgcac
    38341 tttcaagcat cgcgtttttc aaatcaaaga aataatcatc gactgtttta atcttttgaa
    38401 gagtatcttt tcctagttct ctaattttta attcgacagc tgcaatatca tctaagattt
    38461 cacttttctt cttgccatgt aaagttacta agctatcttc caattcattt ttaagagata
    38521 ggaaccgatc tcttgtattg tgttcaatct gatttgcaac acgactcatt tcagattcaa
    38581 aattctttag gttttcaaca attgaatctt ttttattctc tgtttcttcg tggaatgttt
    38641 ctttgattct ttggaattca tcttggaatt tcacaatttc ttttaaaagt tctgcttttt
    38701 taaaatcagc ttctcttaga agcttattgt ctgcttctaa gtatttttct tggaacaact
    38761 gaatattaga tacaatggaa tccgcctgac gtcttgtatc atttaacaat tcatctcggc
    38821 gtgtcattgt ttcaagactc atactttcga tttcagaacg aatcgaatcc acttccattc
    38881 tgagaccacc caagatcgct tcttttcctt ggttgacttg atctaaaata gaatcaaatc
    38941 tatcttgaaa tttcttttca aagaatccaa gtttatcttg aacgataagc gcaacagatt
    39001 cagctttttc ttcaaagcga gtatctagtt tttcaatttt ctctgcgatt gcctcagaag
    39061 aatctctata gcgagtgatt tttatttcta atgatttttc agattctcta acaaaagtat
    39121 tataatcatc aagttttgca ttggcaactt caactttgcc aacagtctcc tctatcatga
    39181 tttcaacttc cgtttttaat ttctctttga aagtatttaa aactgaatta agagactcat
    39241 cgactttatc tgactttgcc tccaagagag attctagttc tacaatcttt gtagctaacg
    39301 actctagaat atcttgcgtg cgagtagaaa gagtctggct gaaagtgcga acgattccat
    39361 ttacttcttc tcttacttct cttacttcct tttcaacatc tttgatattt tctctttggg
    39421 attgaatgat ttgcattcct tcttgaatgt attctgcttc atgtcgaatt tcagaggcaa
    39481 gggttacaac gttttttaaa tcagcagaaa tagaatcgag atattcttta tttgctttta
    39541 aggattccat taagctcttt gtttcatttg cttttgcttc tagatcagag ataactttac
    39601 gagcctgctt gcccataatt tccaagtcga ttgtagtatc cttaacagct tgaataccag
    39661 ccatcgcagc ttggttaata tcttcattca tcttagctga atgttttttg atttggctca
    39721 atcgatagtt cgatcgatcc aattttctaa agagtaatat aacaattaca cttgcaataa
    39781 acggaaataa aagctctgaa cccattgtgt ataaaatatc ccaagctaag atttaagaaa
    39841 tatgtcttat attgcaacaa aaatttatgt ctcattaaaa aatattgatt ttttatacat
    39901 tttgccagca agagaagtaa aaattttatt ctatgacttc aatctgagaa caatcgattt
    39961 gaattcagga aaagatgtat caatccaaga gtcatcatca attttaatat cattctcgag
    40021 caaggttttt agtatcaaga aactcattgc gattcgatga tccataaaag attcgattaa
    40081 agctggttgt atttctttga cttgttcaaa agaataacca tctttttgtt catctacttc
    40141 tatcccgagc tttcttagat tcacaaccat cgcatgaatt cgatccgatt cttttgcacg
    40201 taaatcttct gcatttcgta tttcaaaccc atttttagag aagagtcctg caatcgttag
    40261 aattggaatt tcatcaatga tggagggaat caagtcggaa cttatttctg ttttagttag
    40321 attagatgga tagactcgca aatctccaat gacttcgcca cattcagatc tttcgttctc
    40381 tacttcaatc ttgcccccca tattttttag aactgtgagt ataccagttc gagctggatt
    40441 gagtccaacg ttttgaatta aaatagagcc tgattttgct aataatccaa atacaataaa
    40501 aaaagcagcg gaggaaatat cgccaggaac ttggaactta gcaccagaaa aagcaaaagg
    40561 tggcttcatc ttaaaatgag tctttgtaaa aaactctatc tcaccgccta aaaattggag
    40621 catattttca gtatgattac gagagagttc ctcttcttca tactcaagcc ctacatcaga
    40681 ggagattgcg gctaacatca aacatgattt tacttgtgca ctagcaattg ggcttttgta
    40741 gaaaatggaa attaatttct taccttgaat tttgagagga gcctttccgt ctccagataa
    40801 agattgaata tctgcaccca tcagagaaag gggatctatg atacgcttca taggtctctt
    40861 tcgcaaagaa gcatcaccag tcaactgtgt ctgaattccg acaaggccgg ataaaagccc
    40921 agcagataaa cgaatccctg ttccaccatt gccaaaatcc aactcttcct ttgggcttgt
    40981 gagtcctaat ttgccagggc tgactacttt ataacttcct tttcctagtt ttgtaaactc
    41041 aacacccaat ttagaaaaag atttcattgt gtttaaggga tcctcggctt ctaagaatcc
    41101 attgatttcg gaatttcctt tcgcgaggga agaaaataaa acagaacgat gagagataga
    41161 cttatctcca ggaacttgga ttgtgaaatc agcgggaata ttgaatttag atactagcat
    41221 ccttccattt cttaggaagc actagttctg aaaataatta tttaaaagtt tgcggtttgg
    41281 gtttaaatcc ttttacagcc ggcaaggcaa tcactccctg ctagagctgt aaaaccagat
    41341 ttaaattgtt aataagctta ttcgatactt tagaagggaa ttatttggat agttcttgtg
    41401 cttttgctat gacagcatct ctaccgccag gaactttaat aaacacaaca cattcacatt
    41461 ctttccagcc actgtatgga atttcaggat catctttata gagagcctta cattctttta
    41521 catttacacc ttgtagtttg ccttgaaatt ctcttactac aactgcacct gtagattcac
    41581 catcggaaac cccagaagca ccttgaatgc tttcccctac aagcttacca attagatcgc
    41641 cttttacttt tgttgttgct gcatcagtac aagtactttg catcattgca ccgcttcttt
    41701 tttccatagc ctttggagaa gccttaccga cagatttcat atagtagaac tcaaatggtc
    41761 ttttgtttac atcctcagga ggaccatgcc atccttcaaa ttgtttacca ccctctccca
    41821 cactatgagc ttctttatct ccagtgccac agccagaaac tgctacaatt aacatagtaa
    41881 aaagcaatac tgctttactt ttcgacatcc ttaactcctt gtaataaaaa ttaagattat
    41941 ttctatcatc cttaatactg aaaaaaattt aaagcaaaat attcattttt tagaaattaa
    42001 tttctaaaaa gttagattcc ttccccataa ataaagtttt gcaagagttt atcctctact
    42061 attctttggt cttttttatt acttttctca tgcgaaatct ccgcgatttc ctgctgaagt
    42121 gtatcaattc tctccagtag tatagagaaa actctcgcaa caggatcagg catttgtcca
    42181 tgatccagca tatgctcact atttttctcc tctgacttca caaccctccc tggaattccg
    42241 acaacagtgc aattggcagg aacatctcgc attacaaccg atccagcacc cactcgaaca
    42301 ttattttcta agatgatatt tcctaaaact tttgcccccg aaccaacaac aacattctct
    42361 ttcaaagttg gatgtctttt tcctgattct tttcccgttc cacctagagt tactccttga
    42421 taaatcaaac agcctgttcc tatttcggct gtttctccga tcactactcc catcccatga
    42481 tcgatcatga ttccactggc aatttttgcg cctgggtgta tgtcgattcc tgttaagaac
    42541 cttgcaatat aattcaacat tctaggtaaa agtggaacct tacacttata taagaaatga
    42601 gcaatggagt gaaaccacag agcatgaagt cccggataac agagaattac ttccagatac
    42661 gattttgcag ctggatcatt gtctttaatt tttcttatat tatcaaacat gaatgttgaa
    42721 tattttgagg aaagattttt tgaaagaatt aaaaagagaa actcgcaatt tatttactct
    42781 tcatatcata aacaccctcc caatctaatg ggagtccatg ttttaaatat tccctcgttc
    42841 tttccaaata ttgaagcact gccttatctt ctggaaaact tcgatggagt tccgacaaaa
    42901 cctctcctgc ggatttcata tcaccatata agtaaaaatt aattcctttt ttaaaatctg
    42961 catttgtttg cattttcttt tcgcgaattt ctggatcatc tgcattcatg atctcataaa
    43021 tttcgattgg ttgttttcga cctttcacgc gaactctgtc taaatagcgg atcgcatatt
    43081 tggattgatc ttggatttta cctaaaacaa tagaacttat aatgatggat gcaccatagt
    43141 atttagtgag cccctctaac ctagaagtga ggttcacatg gtcagaaaaa gcatctcctt
    43201 gcatacggtt tgtctcccca atcataccaa gcatcataaa tccagaatga attccataac
    43261 ccgtatgaat cggcaaatac ccattagcca cacgcttttt attataaatg gaaagttcat
    43321 ttaattgggc aatagcagaa tcaattgcat cttctgtctt ctctttaaag acagccatga
    43381 ttgcatcccc aacatatttc acaatgattc cattgtattc acgcactttt ggactcactc
    43441 gcttgaaata ggcatttaca aaattaaaat tttcttgcgg agtcatcgtc tcggacaaag
    43501 tagaaaaaga cctaatatcc gaaaagacta cagccatatc tgctgcaacg ttatctccta
    43561 gattcacatc aatgatactt tctttagata aaaaatgtaa ataatcatgt ggaacaaatc
    43621 taccatagga tttattgaga gtagaaattt ggatatgagt tttgattcga gtgagtaact
    43681 cttccttctg aaatggcttt gctaaaaaat catttgctcc gcactcaaat ccgtatacca
    43741 aatcactgat tagatttttg gctgttagca taataatagg caaatcaccg ctcgatcttt
    43801 tcctacgaat cgttttacaa acctcatagc cggaaagttt aggcatcata atatcaagta
    43861 atattaaatt cacttcctct ttttctagaa tagctaacgc atcctctcca ttgcttgcct
    43921 taagaactga atattttgca agagttaaat gattctctag aacttgaacg ttcacaggtt
    43981 catcatcaac cactaatatt ttaatacttc cttctgcggt tgtatttata attatttcat
    44041 tttgcgcata ctcttcattt gcctctaact tctgaagaga atacgttttg gcaggttcat
    44101 caactggttt ttcatctgat ataggaagag taaaaaagaa tttactacct ttccctactt
    44161 cagattccac ccatatttta ccaccatgta gttctattaa attttttgta atgctgagcc
    44221 caagaccagt tccaccatac tcccttgaaa tagaggcatc tacctgctcg aaggattgaa
    44281 aaatggaatc aaatttatcg gatggaatcc ctatcccagt atcagtaaca actatcgtta
    44341 acttatcttc catcttttgt tttgttgtaa tttcaacttt tcctgattct gtaaacttga
    44401 ttgcatttcc aattaaattt aaaagaatct gctgaagacg attttcatct ccttttatag
    44461 caggaacatc ttctggaatt tggtttaaaa gctgaatcct ctttccgcca agtaaatgtc
    44521 tcgaaagaag taaaaccata tccgcaatag agtgaatatc gactggtttt tgttggataa
    44581 ttatttcatg attttttaat ttagaaaaat ccagaatatc attcactaaa ttagtaagcc
    44641 ttttaccact atcactaatc atttttaaat ttttaactgg ctctttttct aactttcctg
    44701 tagctccatc aatcatagaa tcagcaattc caataattcc attgagcgga gtcttcaatt
    44761 catgcgaagt attagacaaa aactcatcct ttagtttatc caatttctct aactcaacgt
    44821 tttttctctc tagctcagtt aaactttgac taagatcatt tgccatttta ttaaatgtct
    44881 gggacaaatc ccctatttcg tcagatgaaa aagcaggaac tctttcgtct aactttccct
    44941 gtccaaaacg atcagctgct ttttttagaa ctaaaagtgg agcaatcact cttctagata
    45001 aaaaatacac aattgcaagc gaaaagctaa ttagaattag ccctactgta agaccagtat
    45061 ctctaatttt tgtaatcgct ttaaatgctt cttttttagg aacttgtaag taaagttgcc
    45121 atcgatttcc ttgaaaatca agaaatccat tttcactctc tttagcaaaa atagtctcat
    45181 ttgcgcttgc taaaatttct gtttctgttt gttttagaac ggaatttttt ttctgaactt
    45241 tatgattgga ataaattgta tttccctcat gatcgagtaa gtcaatgagc aaatactttt
    45301 ccaaatcttt agtgccttgt ataatttgat gcaatcttcc aactgaaaca taagcagtta
    45361 cagctccaat caaatttccc tctgtgctta aaacaggtgc cgcaaataca atcactctat
    45421 ttcctaattc ctctaaatac aaaatatcac ttgcagcact gactcgatta tctttataaa
    45481 cttcttcttt ccacaaagag tctttacatt tttctcctat cgacagaccg tttgtatctg
    45541 caattttgat acaatcttta tcataatagg caagagaaat ataaaccttt ctctgattac
    45601 ggtaagatat taatttcttt gcaatctctt catccgttac ttttggctgt aaaaatattg
    45661 ggtcagcgct tatcgtttga atatctgaat atctttcaaa aaggactcta tccattttat
    45721 caaacatatg ttctgctgtt tcctttagat ttttttgaat ctgctcttcc accgattgag
    45781 tcattaataa atagaccgaa atactcgttg gaattagaat tgcgagagta aagcttaaat
    45841 acactatgaa tattttaaaa gatagtttca tttatctcca cctgctttca caaattggtc
    45901 atctataatc gactgaagat ccggcatgcg atagagctga cccctttctc gcaaaaattg
    45961 aattattttt tgaccagcag tcaacaaacc aaatctcttt tccttctgat tcatactgat
    46021 actttgatct ggtagaattc ctccgggtgg ctttgctcca tccttgggtc cttgtgaaaa
    46081 atcgaattta ggcgattcat ttatatttgt ttgttgtaat ttatcatctt gcttttcaag
    46141 agcagcttca ttctcaactt gggataaaaa ctttatacca gataattctt ctttgatatc
    46201 ttccttttta cgttttgaat aattaacaaa gacactaaca ccttcctcag gattagagtc
    46261 ataaaaagac tttgcctcaa tgatagaaga aactaaaagt ttaacctttt gtttatgttt
    46321 ttctaaaaaa gccgagtcgg ctaccaaagt atccgcaagt aaatccggat catctgatga
    46381 tttgcctata acagacaatc cttttttgtt tatctctgaa agacttactc cagttgtatg
    46441 ccctgcgtct atcgttcctt tttctaattc ttgaataact ttatgcatcg gtaaatttac
    46501 agcaaaatat tccccatcct gaataccttt tttactgaga agctgttgaa caaaaatatg
    46561 agaagaagag tttatacctt cgaaagaaat ttttttcttt ttcaaagatt ccatatcttt
    46621 aatagaatta ttagaaacaa tcagatcatt tgtgatggag taagtaaacc tatagatgat
    46681 tttcgaaaaa tgtccttcag acgtggaaag aatcgattca gttaaaccag tgcagataat
    46741 atcaagtttg cgttcagcat acaactcact taaattttca tctatatgta actcaacatc
    46801 tattccgttt ttcttaaaga tacctttttc ctcagcaata aaaaccggaa gataactaac
    46861 acgcaaagga actcccaaac gaattttagc attaccctct ttctctttag aacatgcaag
    46921 aaagaggata ataatgatta agcagattaa attaattatt cgcatagtaa gcgccagaac
    46981 ttactctctc ttcctttgga atgatttttg taagcgcagc aaagattgtt tttgcttttg
    47041 ctttgtttcc ttgcttcaaa agagaccaac ctagtccaat ttgcatttca gtgtcagcag
    47101 ggtaatccac aattaaagat tcatattgct tttctgcttc tgcataattt gcaagaacat
    47161 aatttgcata ggctagtgtg cttcgtgcaa aataattttt tggatctagc ttgaggatag
    47221 tcttacaagc aacatccaca tctttgtatt tacccatcgc gagtaacgct cttacatttc
    47281 caagtcttgg ctcaattgca tttggagcaa gaagagctgc cttttgataa taagaggctg
    47341 atttagtaaa atcaccttta atcagtgcta cccatccggt tctcaattga taaaaatact
    47401 cattcggatt gtctttactt aaaccttcca ttgtctccag tgcttttgca aactcacgct
    47461 tagcttcttg tccataggaa tcagctaatg gatcagcgat tatttgcatt ccactcaaaa
    47521 ttaaacatac tattaaaatc tttttcatta gaatctccat acgattgtta gttttgcagt
    47581 atttgatgtt ggtctatcgt ttctaaaatc aagccattgg tcatgactta gcatcagttg
    47641 agccgttagt cgatctgatt taaatccaat ctgtccatat cctccagaat gtaaatcaaa
    47701 gcctgtataa atccagtttt ctcccataaa aggagttctt gctgttccat accgtccgcc
    47761 aatagctaca tagaaatact tccagagata accaaattct tgttgaaaga aaccataatt
    47821 ttttgaaact tttatagaat cagttgatgt tagaagtgga cgaacactga tccaaacaac
    47881 ctctgactct tttactgtca ttgcttgacc actaatagtt gaggatgagt aaaaattttc
    47941 agtaaacgga taatagaatg taaccgttcc ctgaccaccc ttactatatg gagctgatat
    48001 tgccgctcca taaaaagaaa ttgtatattt attcgtgtaa ctaacaccaa attgccccgc
    48061 agctccttta tttaaaaaag atgtattaga ggaaagtccc tgaggagtaa agtgtaatgc
    48121 taaccgatca gataattgat aagatactcc taacgcatat tttgttgtag aataatcatt
    48181 agaggaaatt aaagaatata agttattaac atttgtaaaa ttcccaaaat aataacttgg
    48241 aattgtggag ggatttctta agtaaccata gctagataga taagcaatat taaccggatc
    48301 aatgatatag ttaaatactc cattagtaga atttaaattt tgagttgtct catgtgtaac
    48361 gttagctctt aaattccaac ctgcatttgg actataggaa acccctagac caaattttga
    48421 tccactccca acataatcag aaccattaaa attgtagaca cttgcctcag ggataactgc
    48481 aaaatatgga taaacacttg caccagataa tgaatactga atgcctttgt ggtcgggtga
    48541 catttttttt ccttcttcaa aaagctttgt tgcttcttct ttgttcccca tatagtattc
    48601 acagagacct atcctccaaa gcaaatcccc attttttcca aactgacttt ccaattgctt
    48661 ataacgttcc gaggctttag aatattcctt tttttccatg taagcatcgg ctactctttg
    48721 ttttgcgata tagctatttg gttcaattgc taagactttt tcaccagctg ctatactttc
    48781 atcattctta cccattgcaa gtaacgccca ttgaataccg gcaagcgaat ctagagatga
    48841 ataaagacta tcagcctttt tatagtctgc taaggctaaa tcatattttt ctgtcatcat
    48901 atatgcatag cctcgataga gatatgccat agatgagtct ggatattttt gtataagttg
    48961 atctaataat tcaattgttt taacataatc accttttgcc ttataagctt cagaaattct
    49021 aaactttgca taaaaattat ctttatcctt ttctagaatt ttctcaccaa cggaaatact
    49081 ctcattgtat ttaccactcg cgagaagcgc ccattgcatt cctgacatag tctctaacgt
    49141 tgagtataac tcatcagctt ttttataatc atcatacgct ttactaaaat catttatcat
    49201 catgtatgca taacctctat aggcgtatgc aacagaatcc tgtggatttt tttttatcac
    49261 tttgtctaat gtttcaattg atttcttata attcaatttt tccatttgtc ctatcgcatc
    49321 ttgcactgtc tgcgataaaa cagaactcgt attaaagagt gttaatagta taattccaat
    49381 taaccaaact tttttaatat ccattgatct ccctcattat ttttaatttg aatcgaaccg
    49441 ggtgcagttg aatccggtag taaatctaac tcatataaaa ccgtatttat attcttaatc
    49501 acttttatct tgaaatttag atttgtatct ttttcaatca tttccccttg ccattcaaaa
    49561 ccaacactat cactaaatgg atgaatcaaa tcttttgcta atcgaataaa taaattggaa
    49621 aaatatttat aagacatacg gtctttccat agacttgata tattaataaa aggtattcta
    49681 taatttgcta gataaaagaa aaataaaccg gtattcttct taccaatata atctaagcaa
    49741 gaaaaataat ctgctgaaat gtaaaaataa actctattgt ttttttcatc ttcaataaat
    49801 cgatttccga gtaaatccag ctgaatcttc catttctctg ttataatctt agattcattc
    49861 ttggtgaggc taatatttat ttcctcccca ggagaaagat tgtaaaaatt ttttaaatta
    49921 aagtcagaat gtagattcgc aattacctga tcttcaattg gtatttcatt gaaatgaatt
    49981 aaggttcgtc catttgactc attttgatat tgagtaaagg tgactggtat agtagaactt
    50041 ccaatttcag gggttgattg aaaatgcaaa tgaatatgag gctctaaact tctacctgaa
    50101 tttccagcaa gtcctagttt tgttcccgcc gcaacataat ctccttcttt tactgaaata
    50161 gaatttttcg aaaaatggga tagatgtgaa aataaaaatg gagtatgttc aataattaca
    50221 aaatttcccc aattattttt tgtatctaca tcaccaggtt tgttatcttc taaatgtttt
    50281 actactttga ttacacgacc cgcacaagga gaaagcactg ttagtccgaa tgtataaaaa
    50341 tcttcaacgt tattctcctt accctttctc acttttccct ctgcatcaat tgccatgaaa
    50401 tccaaacttt ccttccatgc atctcggtgt gtaaattttc cattaaatcc ttgtgagact
    50461 ttccatttac cagaaaaagg aagccgaata tataacccac tgactccaaa tctttgaagc
    50521 cttgatttat agtagtctaa atttgattct ggagcacctg gaataaaatc taccgcaaca
    50581 attcttctat tccgcaataa tttcacagaa taaacaaata gaagggaaac acttgtaaat
    50641 ggtaaggcta aaattggaat gctatatggt gtaaataata cacttgcaaa cgatgacact
    50701 aaagcacttg caatagaagc aaggccagct acaataaagg tggatggact aggaactata
    50761 aagataccgc ctaccgcaat tgctgtcaga atataattaa agccgactgc acctgtcgaa
    50821 atatttccca tcttgccttc taaggctaaa tgaaaactaa ttccagctgc aaacccaatc
    50881 acagacagaa caaacgcaat acgactggaa gcaaataaaa taattgcaaa acaaagtcca
    50941 gcccatggac tagactgaaa aaatattcct ccaagactct ttagataata acttacatag
    51001 cttggaagtt cagggaaata tatatctaga ggaaaaggag tattagccac tcgatacttc
    51061 aaaccattat aatcataaaa ggagaaatat acaatgatac tcacaatcac aaaaggaatt
    51121 gataatacag gtaagcctaa aaaataagag aaggcatact cggatgctat tgttaaaaaa
    51181 accaaaagca cgatagatac aaaaagtata aaaactaatg ttgtatctac agagtgaacc
    51241 agattaatag aaagcccaac aagtaatgcg ttaaacccca aagagccttt ttttattctc
    51301 tcttcatgca cacctaaaaa ctttgctaat aagacagcaa aaataattcc aagcaatccc
    51361 aagagcccag cttcataatt aaaaagtgta gccaagaaaa taaaaagccc caaaacgggg
    51421 cttgaactaa ataaaatgga tgaataggaa gaaagaatag aaaaataaag agttttaaaa
    51481 aaattcattc cttattccta ctccatctct attgaattga gatcatccac agtctctctt
    51541 cttctaattt gcttgacatt accattttgc tcaatcataa caattgctgg tctcatttca
    51601 ataaattgca tccattgggt aacattgtat gcgccaacag gatgaataac caaatgatcc
    51661 ccgcgagaca tattaggaag taagcagctt tctcgcacaa cgtctatatt catacacaaa
    51721 ggtccatata aagttacatc ttcatatgct ttagaataat cttttactgg agaaacttta
    51781 aactcatacc aagtagatgt ataaagtaaa ttcagaccgg catttaaaac aagcgccctt
    51841 ttcccattgg gcatagtttt agatcccaca atactgctta ctaaaaatcc cgactcatcg
    51901 actaacgcac gaccagattc taaaaataac ggaggaggtt cctcaggggc aaatgaatca
    51961 tagatggaag aacaaattgc ttctgcatat tgatcaaaag aaggaagtaa ggaactacct
    52021 gaaaaatatg tgctttttaa tttatttgca gatacaaacc ccccacccat atctaaatat
    52081 tgaagtggtt gacgaagttc atctcttact tttttataaa agtcagtcaa ttttttggct
    52141 gctcttttat aagcagatgc atccatcata aaagttccaa tgtgagaatg aactccgact
    52201 aatgcgagtt tgcctccact atgagcaatt cttctggctg cttccaatgc atggccagac
    52261 tctaaattaa aaccaaatcg actccaaagc ggatagatac ctgtatccat attaatacga
    52321 atcgcaacat ccactcgctt tcctaattct tccgaaattt tcaacaaatc tctaatttca
    52381 tctaggtggt cagcatgaat tcttgctcct tcagatactg caagttttaa tgcgtcatac
    52441 ggcttatacg gaccattaaa tataattcta tttcctggaa ttccattctt tcttgctttt
    52501 tgatattcaa aagatgaaac aacttcagcc caaaatcctt ctttatgaaa tactttacaa
    52561 atagcattta ggtaatttgt tttataagac caagtaggtt gaaaagaagg gtaacgagat
    52621 tcaaaagctt gtttatagtc tgcaattttc ttttttatag tggcttctga aaacacaaac
    52681 agcggtgaac caaatttttc cactaaacta tcgatggata taccgtctat cgaatgcatc
    52741 gtttcaggaa tattaaatcc accaaattta tttgcagcgc ccatgtattg cttgcgaatc
    52801 acaggtcgaa catacatacg tttactcatt tttatctcct ttatcgtttg aaagcaaatg
    52861 acctttaatt gttaatgact caactgattt cattggaata atctcatccc aactatgtct
    52921 tacaaaaacc tttccaacat cataatcaga aaatggtgtc acagtttctc ctaaactgag
    52981 tcgaagcaat gcgtcaggta gattctgacc tgatgcggtt gcaagatata cccaagctgg
    53041 aaaccttgga ttgatttcta ataaatacat tttaccagtc tgattttcaa aaatgaattc
    53101 caattcacat ccactattcc atttgatata atttagaatt tttttagagg tagattttgc
    53161 ttcctcgtta ttgattgtta cacctgccca agcctttcct ttatcagtta gaaatagctt
    53221 tttcataaca acagcaccaa tcatttcccc ttcttttgca agagcgcaaa cattacactc
    53281 ttcacccgct atatattttt gaaaaataac aggaactccc catttaccag ccattgcata
    53341 gaaataacct acggcttcct cataattatg agccaaatag gcttcataaa aaacgccttt
    53401 cacaaagaga ggaaaaccaa tcttatccgt aactggtcga attgcagata cgtcttgaac
    53461 tgatattgtc tctggaagta atacacctgc atcttctaaa gattccttca aaaatgtctt
    53521 atctctagtg tgaagttctt ttcttcccgg taaaaataat ttgattcccg cattatttag
    53581 ttggttggat atagagatat aattatctaa ttcagaatca agggtaggaa taataacgtc
    53641 tattttcttg atggaattaa tatgcattat ccgctcaagt aaaacatcag gaccagaact
    53701 tggataagga agcagaaaag aactttccaa taaatgactc ataaagtttc caggctctaa
    53761 agctccatag gcaaagccta ctaattctgg atttaatttg gattctcgta atgatcttaa
    53821 aacaggaact ccaggacctg gactatcgac agcattcatg ccactaacgg ctacaactaa
    53881 cctactcatc taataaaccc caattcggtt aactgtataa tcatatcaga gtaatctctt
    53941 tcaacctcat cttgttccac ttcatattca gcaagtattg attttatgat ttcatctttt
    54001 gttttgtctt gttttaaaag tcgaactatc acaagacccg tttcatttag tgtgtaagtg
    54061 ttaccagttg taggatcaaa tacaaatccc gtatcgctca gtgcaaggtt ctttaatgcg
    54121 tctgatgcca ttttttcctt ccttattgca attctatgaa aacaacagag agaatttttt
    54181 ttaatatatt ggctagaata ttttaacaaa aaaaaatttt tcttcattaa acaccaatta
    54241 ttcgcctcaa aatctttcca attgctctta ctaataagta tacaaaaaaa taaatttagg
    54301 gtcatccaat ccaataagtt tgtttttttt attcattttt atttcttttt ttattaaatt
    54361 gagaataaac aaagttgaat aattcgaacg gtatgcaatc atagaatttg gaattagtaa
    54421 tagataatga atacacaaac cgattgggga aaacttcaaa acctacaaaa gacttttaaa
    54481 agaggatcag ttgttataaa agagggaact agtccgaatg ctctgtatgt cttaatgagt
    54541 ggtagactca acgtcattag agatggaatt aaaatctctc aaattaaaaa acgaggcgaa
    54601 tacattggtg aaattgcagt tctattaaat atccctgcaa gtgcgactgt aaaagcagaa
    54661 acagaatgtg ttttgattga aattgaaagc agcaaaataa taccattcct tcatcatgcc
    54721 ccagaaattg ccattgctct tgcgcgcaag atggcagaac gattagtaga attaaatgca
    54781 aatttctctc aattgattga taagtcttac agaccagact ttataaaatc attgcagaat
    54841 agacaaaaac tggaaataac aatcaaacca gaatatgaaa ttaatttaga aaaattaaga
    54901 tccttgtttg taaaatatgc ggcaggaatg gatgtcttaa cccaaggtca atatccaaga
    54961 gcattgtata tattagtttc tggaacctta gacattataa aaaatggcaa ggtcattgca
    55021 gtagaagata agcctggata ctatttaggt gatgtcgcag ttcttcgaaa tacattttcc
    55081 aatgcaactg taacaactag aacagagtct acactcattg agattggcat agaaaaagtt
    55141 gaagcctttc ttaaccataa accagatatt gcgatctcgg tagctgtcaa actagcagaa
    55201 agaacgttag ctatcaatga actttttcta gacttacaaa cagatgcaat caaagaatat
    55261 aaaaagaaag aagaacaatt agaaaaaaag aacgcagtta acagttcaaa taatacagcc
    55321 ggctacaaaa agatcgatag actctcagat gaaattgaaa agttaagaaa agaggggcaa
    55381 gaaatttata gaatgttagg cattgaatca attgaataga atatctcaaa aggattcaat
    55441 gctatctgaa ttgaaattta ttacgtaagt cagttgataa aattttagtt tatgactttc
    55501 tttgtttcac tacactaaaa ataggaatat cactatctgt ttcaactgtt tggatctgaa
    55561 gcatttcaac tagatcgtca cgcagtcctt ttaaaaatcc ttttttctct tcttgttgtc
    55621 gattatcgat ttgttctgtc tctaaatgaa tcgactccat ttcagaagat gtatcattca
    55681 tatttagaat aaccggttga taggttttct cagaggcata agcgcttggg ctcttcagta
    55741 aaggagtttc ttcaggaaga aaggacaatt caattttatg aaaggtagga acaggaccct
    55801 ctggaatcag agatttagct ccatttgaat ctgttttaca tctaacatct gtttctccat
    55861 gacaaaaata acagctaaca gtaaagacat tagaatgaga aattgaattt gccaatttgg
    55921 actttttacc acaatgaggg caattaaatg taatttctgt gacaggggat gtagtattca
    55981 tattctctat agaaatcggt ctatttttta tatgcgataa taagaaaatt atgtaagatt
    56041 agcttgaatc ttaaaaattt tcttattcaa atcctttata taacgcctat tttctttgcg
    56101 acggcatata aatttacact ccagcccact cctaagaaat gctttggaac aatgatttct
    56161 tctgtgcttg gatcccagaa gtctttaata aaagcctctg ttgtaacttc cctagcaatc
    56221 ggtaatcccc aaaaattatc ttgttctttg ttctccattt gtgtgtcctt ttttttattt
    56281 tgcgtaaata tgttttagta aattcaattt taagtcaata aatttaatca aaaaatttca
    56341 tgattattca ccttactcag ggtgaaccaa acgttcagat gaggtttgct acgagcggag
    56401 gactttcctc cgatcgcttt ttcgctctcc tgcagccgag ctataccgca ggctattgat
    56461 tttattaatt cattagcatt ctctaatttc ttaaaaatca ttgtctattc ctcaaattaa
    56521 aaattttgcg taagcttaat taattattca aactatcaac tttgttgtta acaaaatttt
    56581 atgattttga tttcttaact aactcaaaaa actcagtaaa taaatattct gcatcatgag
    56641 gtcctggtga actttctgga tgatactgaa ctgccattac tgggagagag ctagatctaa
    56701 ttccttcaat cgtttgatca tttaaattta tatgtgtaat tggctctgcg gaagaatcct
    56761 cgccaacgac cgcaaaacca tgattttgcg ctgtaatttc tactctgcct gttgaataat
    56821 ttttcacagg ttggtttcca cctctatgac caaattttag cttagtagtt tttcgtccca
    56881 ttgcaagacc aatgatctga tgaccaaggc aaattccaaa aagtggcttc ttcttttcga
    56941 taattgtctt tgcagaatga attccgtaac tgactggttc tggatctcct ggaccattgg
    57001 aaagaaaata agcatcaaaa ttttcttcca taattttctc tattggatat tccgcaggga
    57061 aaacagtaac cgcaaatcca gctttattta agcaatttaa aatattcgtc ttaacaccat
    57121 agtcataaac agcaacacga tatggtttat tttctttgga accaaactca taacgctctt
    57181 tcgtagaaac tacttttgct aaatccattc ctttaatgcc aggaaagtct tttacttttt
    57241 tcaaaaactc atctgagtat ttgtctgcaa taaaaattcc accattcgga gaaccatttg
    57301 tgcggataaa tcttgttagc ttccgtgtat caattccctg aatagctggt attttatgcc
    57361 gaattaaaaa tgcagaaaga gtttcggtag atctaaaatt agaaggaaca ggaacatatt
    57421 cttttacaat cagtccactc acttgaattt tgtcagactc catatcttct gcacaaattc
    57481 cataattgcc aatcatagga taggtcaggg ttacaatctg tttgcaataa gaaggatctg
    57541 ttagaatctc ctgatagcca gccatagatg tattaaaaac tacttctcct acttcagagc
    57601 cctcataacc aaaggactcc ccctcaaaaa cttcaccgtt gtctaaaact aaaaatgctt
    57661 tcatttgttt ctaacatatt tcatattgtt gcattggggt caaccaaatg ctttcaccaa
    57721 aagacgattc atttacctta atttttgggc acatctctga taccaattta atcccaattc
    57781 taataaatga gaaaaaatac ctagtcccaa agcatgtaaa tctactaaga gcatttcatt
    57841 atattgcggc cacttcagaa gaatatgact taaaactcca aaaacactgt tgggcgggta
    57901 gctgtgaaaa ttgtaaatgt agtttcaaag actctcaatt aggagaggca gaaggacttg
    57961 cctgtcaaat ggatctggaa gtaaacttaa aaatcatctc ccttccgaaa acgatgaaac
    58021 gaaaaattaa atttgctgct gaatatcaaa tttaaaattt atgcgacttc ga
//
LOCUS       DAOQOS010000016        55972 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_212_length_55972_cov_6.968328, whole genome shotgun sequence.
ACCESSION   DAOQOS010000016 DAOQOS010000000
VERSION     DAOQOS010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 55972)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 55972)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55972
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_212_length_55972_cov_6.968328"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            87..599
                     /locus_tag="PLF66_05330"
     CDS             87..599
                     /locus_tag="PLF66_05330"
                     /inference="COORDINATES: protein motif:HMM:NF019235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1564 family protein"
                     /protein_id="HMZ63496.1"
                     /translation="MTNVFSNAKLSFPRMNFSLNDFNSTLLIPEKYQSLFIKKIEKHK
                     GIRSYVAYLLSKYQVLIKNGLIPGYSNVTTKYQEKGQNLRKFAFRPRPEDWAELKLYR
                     VSFGMSISAFFVYLLIADSVDFAETISDYLTTVGISLVSNLNLTAKVHLWNQKTFYTK
                     VLQYRKSKYP"
     gene            complement(655..2460)
                     /locus_tag="PLF66_05335"
     CDS             complement(655..2460)
                     /locus_tag="PLF66_05335"
                     /inference="COORDINATES: protein motif:HMM:TIGR02231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mucoidy inhibitor MuiA family protein"
                     /protein_id="HMZ63497.1"
                     /translation="MYKKSIFVFFILICINLLAEPISKEGKIQDVVLYRNQALVTRVI
                     ELDLKEGSHEITISKLPSELIQNSLYAEAIGAEVRAAKLKITEVKIDSDPKLAALDDK
                     IEKLNQEFIKLSKLSEVNRLKLAFLTKQEDFTVTSEKIELNRGLLNAETIKQLTLFHF
                     EQRRDLALEEIKIQEDIKALNRERDLLARERKQLVKTSLKSMDASIFLDKIGNGKCEI
                     KLFYLVQNAGWSPIYNFYSKLGSKTVKVEFNARIQQITGENWENVNLTLSNATPALSA
                     LAPGLSPFRISLSTLGNSIGLDDLKSASQGISKKMSAAYNKQIGAQNWNETQEGNWAM
                     NSAANEYQNLELVAKDEDLNVLKKDSKQNNASPSVSFNLKSKVSFSSKNEQQLVKVDK
                     SELSAKFYNVAIPLLTSYVYREAEIENEIFENLLEGPVSVYLDEKFVGHGEISNVAKG
                     QSFVMGFGIDTQIRARRELVEKKEKILGGNKELNFKIRMILENFSKKPTDLRVLDRIP
                     AEDEKENVRITLDLKNSALSSDELYQQYERIRGILRWDLGLQADSSATKAKTFEYSYK
                     LEFDKNLQVSFPTPAKADQMKTEFEEIQRMKYNRR"
     gene            complement(2520..3254)
                     /locus_tag="PLF66_05340"
     CDS             complement(2520..3254)
                     /locus_tag="PLF66_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002977545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HMZ63498.1"
                     /translation="MKIITWNCNGGFRKKFKRLLGFNADIYIIQECEEIGKYESFQKE
                     LLENSIENFLWTGENKNKGLGVFSKKNIEIEKLDWNFSYRGRTLKYFLPFQVNKEFRI
                     LAVWNHHADAKVFSYIGQFWLFLQNNKKEFKNIIICGDFNSNSIWDKWDIWWNHSDCV
                     RELEALNIFSAYHKLTYEKQGNEKTKTFFLHKKKEKGYHIDYVFASENFLKKSIDLKI
                     EPFQKWIDMSDHVPLIFTIDTSIYSN"
     gene            complement(3285..4754)
                     /locus_tag="PLF66_05345"
     CDS             complement(3285..4754)
                     /locus_tag="PLF66_05345"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HMZ63499.1"
                     /translation="MNHNYFNQLFIYQLNTRIFCIENGVRVLGAPENFFRSPEVFASD
                     AIWLMGVWSPSQKSIQICSEHEGLNHEFRKTLSDLETKDIIGSPYAIFKYEPNPLIAE
                     HFLELKQFKYKLNSIGKKLILDFVPNHMAVDSPLIDKYPDLFLYKKEENAEVCKNSFQ
                     HKNGRIYFYGRDPYYDGWTDTVQWDFSHPDTIKLHKKILSNISEVCDGVRCDMAMLPL
                     EDVFEKTHGIRALPYWKELIDSVKERYPNFLFIAEVYWNREYDLQQLGFDYTYDKTLY
                     DRLKSREAEGIRLHLEATEEYQSHSLRFIENHDEERAVSTFKSWANSCFALLCFLPGS
                     VLYHQGQRQGKQIKIPVQLARGPKEEINLESENFYDRAFYRIQKRKNKNLQLETFELK
                     AYMDDYSLKNCIVRSVLNLDDNLVEILIFNTYPHQVIGALHLPEQIREKVLQYKQDEI
                     ILKDVLTGETYSREKEMIERGMFIKLESGQAHWFSFHLR"
     gene            4818..6536
                     /locus_tag="PLF66_05350"
                     /pseudo
     CDS             4818..6536
                     /locus_tag="PLF66_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010570485.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
     gene            6735..8036
                     /locus_tag="PLF66_05355"
     CDS             6735..8036
                     /locus_tag="PLF66_05355"
                     /inference="COORDINATES: protein motif:HMM:NF021523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2252 family protein"
                     /protein_id="HMZ63500.1"
                     /translation="MSDIKTIVDTDRTDFIKKEILHWNSFIPEQDQFKKKLQDMKESF
                     FHFFRATNHLFYKDIDSGLIQIPTEWKETPRIQTWIQGDLHTQNLGFFENAKGEIKLD
                     VNDFDESYIAPFYFDLIRFVTSIFLQKESINFNMSHKEAEELAEHFLEEYQETLEEIS
                     DEGRELELIESNLDGFPKDTLEELRDKKSNSTLLDKWTKVTNGVREFEFSNPDLTQLL
                     DEEHREITSFWYTYLESLSEFAQERGSNFFQILKLARRLNSGLGSQGVYRYYALIQAD
                     GNPLLLEIKEQRLPSLFLLKDLSEAEYNTFFPNHAERTKTAFRTMVANPDGFLGTLST
                     SKRSYLVTRISPYKKKLESKDFHSESDFKKYLKHSARAIAYAHSRAYKSILIKSTSLS
                     FANSALSAIKAWAGTKQTIMNLGEKYSEQVKSDYDSFLKSF"
     gene            8040..8681
                     /locus_tag="PLF66_05360"
     CDS             8040..8681
                     /locus_tag="PLF66_05360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63501.1"
                     /translation="MTELKQEIDLFLRRSFLDPFKRRLVINSKLIQYEKKSIPCSEIR
                     QVRYGILQMYINGIKANRIYEIGLTTNNFKPIRITFQSSRIFFKNEKLENTYNDIIEC
                     LWNQVTKRLVTEAIENLEKGKTFSMEKIEVHPKGIQMQVRSWLFKKNIYFVEWKDVRK
                     YSQDGLLCIYSEQNKKAKINLNYQKDWNTPVLSSLLDYLWENGRAYALSEDRF"
     gene            8813..9193
                     /locus_tag="PLF66_05365"
     CDS             8813..9193
                     /locus_tag="PLF66_05365"
                     /inference="COORDINATES: protein motif:HMM:NF019301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family membrane protein"
                     /protein_id="HMZ63502.1"
                     /translation="MSKLFLYTPRILAGIMMLLFGLDKLFPFMPHPPAAEAGILFKSA
                     LVATGYFFKFVGLCEIFVGLAFISGYFVPIALLVMAPLCVNFILYHIFLDPANIAGAL
                     FVTAVCLFLTWQNWEIFKPLVKPR"
     gene            9263..9505
                     /gene="tatA"
                     /locus_tag="PLF66_05370"
     CDS             9263..9505
                     /gene="tatA"
                     /locus_tag="PLF66_05370"
                     /inference="COORDINATES: protein motif:HMM:NF014470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HMZ63503.1"
                     /translation="MLPPLAIFNLGPWEITFILLLALLLFGGKKLPSLAKDLGTGIKE
                     FKKSLSSASTEDIEPPQPPVTPVQEEPTKKKAGKKS"
     gene            9604..10377
                     /gene="tatC"
                     /locus_tag="PLF66_05375"
     CDS             9604..10377
                     /gene="tatC"
                     /locus_tag="PLF66_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003000360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HMZ63504.1"
                     /translation="MTLPDKQTEEVIPNDREKYMSLGDHLEELRQRLIKCLLIVGLFT
                     IVFMIFGSEVHSIFASPYKKVLGEKATFYQVKLMAPMLIYLKTSFILAILFSVPLLLY
                     IIWGFISPAVDIEMERYGKLMIVFATLLFWTGLAVCWFTVFENFLRVFLVMFQPGDID
                     TKLPIDEYYDVFFNLHLIFGISFELPVVLILLGRIGLISSDFLLNKWRETTVILSVFS
                     AVLSPGPDVFSMMMLFAPLLVLFFLSIIIMRIAETRKES"
     gene            10421..11773
                     /locus_tag="PLF66_05380"
     CDS             10421..11773
                     /locus_tag="PLF66_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015679008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMZ63505.1"
                     /translation="MNIISLITFRYIRGSRVFGLLSLKSRLSFIVMMVGVSLLIVVLS
                     IFNGFQKQVKESLWSGGPHITIENTQGSGDIQKYERMIELLLQNPEIKERVISMQGNI
                     TSHGLLQNNNTFVPVMIRAVPVPNEEELVENKVPNFPKLDYYNRDEVKDLNSKNLVVI
                     GKEMSLLYHYNLGRNITLAVPSGSFNVSRGVELSISNFAVTGLFKTGYYNYDSKFIFM
                     SLPTAQKFFKMKNSVNQITVKVKSLDDLQLCKQLILQTISSSDFDSSITEGTYFSVRT
                     IAEEQANFLGALRMEKTIISNIVFLFIVLAALGMVASVYSLVRSKRKSIGTLKALGLP
                     SSSILLIFTLNSMIIGLFASIIGGIIGIYYANNLESIIDGLSEVINTFGRAFSKGEWH
                     EIKLVPKDIYYFDHLPVDIDISFIFMVTTAATILSGLAGYFPARWAANLDPVETIKND
                     "
     gene            12073..12291
                     /locus_tag="PLF66_05385"
     CDS             12073..12291
                     /locus_tag="PLF66_05385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63506.1"
                     /translation="MKGAIHNYLIDYFKRLGLSEDSLSGKTKIRSIRIQISLKGLNLA
                     NYGPDTIDNPVELNKLKSILESLKLGAA"
     gene            12291..13427
                     /locus_tag="PLF66_05390"
     CDS             12291..13427
                     /locus_tag="PLF66_05390"
                     /inference="COORDINATES: protein motif:HMM:NF020084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HMZ63507.1"
                     /translation="MNSLITSSIYKNSIDLFTAIENRNVGLIICFYGSDYNYTELYHN
                     LKKTKIPFIGCMDIARFANASYQFETDSVAVMTISNEILEKVSIIAYDMRKTTSNHSM
                     YQTSKDEYSRALNTLGFDLQSPDMEREFSINLLYGLQSANSVLSAQNEVSLFLQTVGG
                     SSGGKLDFKSSSVICSKGMGSIGATAIVRLKPEYSFMIDRTSSFDSHEGKLVVTKLAN
                     PRYILEFNGKPAAMEYARVIGKNVSDLNPEIYALYTLGVEPGDGERLITSIMKDDGNN
                     GLLTYNDLLEGTELNIYKAKTQFEDRAAKLKMLDQNKLLGYISFDCVLCYLARDANKE
                     IEKIAVLYEETLPGIPKIGFGTFSENFCGANVNQTETYLAITRK"
     gene            complement(13674..16238)
                     /locus_tag="PLF66_05395"
     CDS             complement(13674..16238)
                     /locus_tag="PLF66_05395"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HMZ63508.1"
                     /translation="MKSKFRYFFLLHFLLVCFPIFSVEQVDLLKDCNSENQCKQRPWY
                     IIDNFKEDYIQSDTVPEGSKEIEKFPVSVQKIYPQTESIDFTLRTEFKVSDEYFNEKI
                     TTGIYLDAIGDVYTIYLNGKMIGQEGKVENGKVVLSRYGNHIKYPLNFSFFKKDKNIL
                     ILHIQGNPKFNLTGLFRANGYYLDEYDKILISSRDRTGIILIFLYLFFGAYHLFLYIK
                     RRHETYNLAFSILSLSAFIYLIGRYGLFFEYGLDSTIGYRIELMSVYFIPLSLGIFLE
                     LLFFGKIGRYFKGYILYSALLFIVTPFVPQHFIIYVLRAWQISALVSAIFLIYVFKVA
                     IQKKTDSAKRLMLGLTIMIFSAIFDVLDSIFFKTGLILTRFGFFSFIVGIVGILANRF
                     LNLHETVENLNKTLEDKVIQRTKELQETLSQVKLLKEQQDGDYFLTTLIINPLASNAA
                     NDEKIDLKILIHQKKKFEFKNRMHEIGGDICISDNIFLKGRKFTAFVNGDAMGKSIQG
                     AGGALVLGVVFKSIVSRARFTSQDTVYPERWLKMAFQELQSVFQSFDGSMMISVVMGL
                     IDNDRGFIYYINAEHPFSVLFRDNKASFIEKEMNLRKIGIMTEELSGDFQVNTFKLEH
                     DDVVIMGSDGRDDIKIGMDDFGLRLINEDENLFLKTVEQANADLEKIFYLTQTKGELI
                     DDYSLLRIEYKNTDNYLEEETNDSLHLKELYESGNISEAEKIIEKLLLKRQINRTVLK
                     IRANIAYSKGNFAKAGEYFSEYLDLFPEETNFLFIAANCYKQVGKLESAADYAERLKL
                     REPRNVSNLILLTEIYIALKNIHRATAMLKNAKLVDRNNPRVLELDALIHQIDILV"
     gene            complement(16514..17881)
                     /locus_tag="PLF66_05400"
     CDS             complement(16514..17881)
                     /locus_tag="PLF66_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008589217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate phosphodiesterase"
                     /protein_id="HMZ63509.1"
                     /translation="MKKVELESEDDTYDKVLQDLQDLESFKKKFIDTHRGRPIFMVRM
                     EHIKGISLADFIHFIKHEIQNLPEIKIVDYGFYFVENKRSLLLGIASGGDIADDAFPN
                     LDSHFGRIHQENLRKKKIEFSYGISRTQCNYISSMHEIFDELYKTSEKNLNDNLVRWG
                     WTYFNKANSYISGNAHEAMIQPTVCYDSKEKTFSVKGGEVFVGGGAYKGYKDLISDIP
                     TDQDIRRVELLILEKLILASENAPGLLKFNITPQSLIDTFSNHEKVNRFNSLIRSRNL
                     LPKNVRFELIEKHYEEDEFLLKDVCRCFWDYGYSFAADDFGVKSSSHQIVLDLGIMIQ
                     EFKLDPISFKFKATEDKVKFLDNLAFIDYCKKLADNREAVITAEAVGDLETLKFLMEH
                     QIGQYQTFILFNKIPLTQYKEEFESLKSIPVDVIFEILSHSKLLKEQKKEGHIFHVAK
                     KNKLF"
     gene            complement(18265..18639)
                     /locus_tag="PLF66_05405"
     CDS             complement(18265..18639)
                     /locus_tag="PLF66_05405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63510.1"
                     /translation="MKSNLTIALTIASLTLMVSPIFSISSDLESSLLEKALIEGAVTK
                     EQKTAVAHYLNSVAAEKKKLADSYREMATVNYGGKALTQELRVKKLLNLAESLDKLAA
                     SYENLANNYSPAEYQKLAKLEE"
     gene            complement(18914..20452)
                     /locus_tag="PLF66_05410"
     CDS             complement(18914..20452)
                     /locus_tag="PLF66_05410"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HMZ63511.1"
                     /translation="MTKNKIAPEEKKFYDLINNLQIEIRLGKNIFDILDHILEEILSI
                     FEASTGSISLADPQEKVLTIACAKGIDKEKKIAAKFPFAVGITGASAASKKTIYAPDV
                     TKEKRYVKLIDSVASELAVPMISGDETIGILNLESHYLDHFSKKQIAWAEKIADELSK
                     ALVQNTFYREAMERSKQEQDFLNSILGYDAQIIFLKTRIRTVAPSDASVLINGESGTG
                     KELVAKSLHYLSSRKSNPFVSLNCGALNENLLESELFGHVKGAFTGADRNVIGRFEAA
                     NGGTIFLDEVAEMPPSLQVKLLRVLQEGEIERVGDFKKIKVNVRVISATHKDLAQEVE
                     KGNFRLDLYFRLGVIPLRLPPLRERRGDIPLLVHHFLLEFNKRYGKNKYFDLSTLDLL
                     TQHTWPGNVRELENAIQYAAILCPNDNITPDFLPDNAIRGSDRLKEETSQSKALSSPE
                     EVEIDATDLNLEKALLKVETKLIQEAMRIGKTQEQAAKLLGISRGSLQYKIKNNPNIQ
                     NLID"
     gene            complement(21461..22564)
                     /locus_tag="PLF66_05415"
     CDS             complement(21461..22564)
                     /locus_tag="PLF66_05415"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HMZ63512.1"
                     /translation="MENMKPDRGNHFKKTKSGVPNIFYENTDRISSVRLFCVYYGVTP
                     RVLFLKGLNLSKCLEFFNKMDSNQIEDKREVKYFDRDKNQFELDCLFLLFKTKLLIEI
                     EALSENTAWIRISYGREAEALVNEFVKILIQFKKRKPKLNHIHLIIPGKFNALEKASI
                     QISKPKIKIETHYNSDLELLHKDILKKIKQKNESGLFLFHGQPGTGKSTYIRKLIHLV
                     NKTVIFISPRFASSLDSPDFTKFLIDNPNSIFVIEDAENLIVSRDAEDNSAISMILNL
                     TDGLLGEGLGIQFICTFNTHLSRIDKALLRKGRLKALYEFAALSVEKSNHLLESLSLN
                     YKATKPMTLAEIYNLKEEKFDFKGEKRTIGFAA"
     gene            complement(22612..22836)
                     /locus_tag="PLF66_05420"
     CDS             complement(22612..22836)
                     /locus_tag="PLF66_05420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63513.1"
                     /translation="MKSTIESTTLAPIEVAIANKDTARCKALYNSFLDYSQENKDDLP
                     FMIFLFHSIESRGITVTQEIIDNIRYIASI"
     gene            22984..23982
                     /locus_tag="PLF66_05425"
     CDS             22984..23982
                     /locus_tag="PLF66_05425"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HMZ63514.1"
                     /translation="MAKETTTKQERILIALEYACLLFYEWSNGKKKKLEDFYGFLQSF
                     DSLVHSENKKMPDPQNLERNYRNYLSDLEANFDLTVSLKKKTVQFNSDDSLEEIAFNV
                     LSFYIRHAFLKQTEVHNDSSIKKFIRYRLILFPSKKEESFIMEELYLFVFIRYAIQYN
                     LKLEGSYKKLMSNSISTRRIYPCYLSTDSQYLTLVANDISDNTKKQFILANLHLKPKI
                     SLLNEFIKSQRVKDHFNYKKFKQSKEGRFQREEIFYKIRITSYSLEHLLLNYDFSIKI
                     LEDRGSWKIIELTTSDAIELQKALFAYDVYAILLEPKEEIQNFREKLQRILKENCI"
     gene            24015..25040
                     /locus_tag="PLF66_05430"
     CDS             24015..25040
                     /locus_tag="PLF66_05430"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HMZ63515.1"
                     /translation="MVNSNQSKPIIVAILANSIIAIAKAIGYLFSGSSALLSETIHSI
                     ADVMNQSLLLIGIKRGEQEATNIYHYGLSQERFFWNLVSAMGIFVLGCGVTVYHGVHD
                     LVAGAPHKQGGIEQIIIQIILFISMLVEGYSFYVVIGEIKKQASSRTKSFFEYLREST
                     DPSISAVFWEDFAAILGLLLALVGIALTEFTGNNTFDAFASILIGLLMGWIAIHLAIE
                     NKRFLLDRAIPEYELKQLLEVLDKNPNIKSYKDVRTVVLGPNRLKFKAELELDTEKIF
                     EESVKKAAKKVRTTLKHVKSDEELEAVFFNLGNTLYANKLAELEKIKKQLKDKLPNLQ
                     HVNLVIS"
     gene            25051..26073
                     /gene="gmd"
                     /locus_tag="PLF66_05435"
     CDS             25051..26073
                     /gene="gmd"
                     /locus_tag="PLF66_05435"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004762820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HMZ63516.1"
                     /translation="MKKALITGITGQDGSYLTELLLEKNYEVHGIVRRSSLFNRNRIE
                     HLKDNPNLHLHYGDMTDSSNLNRILEKVQPDEIYNLAAQSHVGVSFEVPEYTAEVDAV
                     GTLRFLDAINETGVKTRFYQASTSELYGLVQEVPQTEKTPFYPRSPYAVAKLYAYWIV
                     VNYREAFGIHASNGILFNHESPRRGETFVTRKITLGVAGIKAKKMKTLTLGNLDARRD
                     WGYAPDYVRMMWMMLQQDQPDDYVVATNEMHSVREFVELSFSIAGIKVEWKGSAEKEI
                     GINAATGETIVAIDPRYYRPTEVEQLLGNPAKAKAKLGWEPKVKFEELVKIMTIADCE
                     AYGIKL"
     gene            26303..27289
                     /locus_tag="PLF66_05440"
     CDS             26303..27289
                     /locus_tag="PLF66_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002770973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HMZ63517.1"
                     /translation="MIEVEHLSKHFKIKKRKPGILGSIVSLVHTDVEYLKAVDDISFT
                     IQPGELVGYIGQNGAGKSTTIKMLTGILTPTSGKIKVHGLDPSKDRRFNARQIGVVFG
                     QRSQLWMDLPVEESFDLLRSIYKIDEKIYKQKLDFFFELLGLDEFFKQQARKLSLGQR
                     MKADLAASLIHSPSVLFLDEPTIGLDLLVKEKVREFIRKINTEDKVTIILTTHDIQDI
                     EFLAQRIIIIEKGKKEYDGDIASFNKLLGNDSIVTIHFVNPVKKLELPSPFTIKKKIS
                     DQQYSLDVPDNVPLSGLLETLQKQGLQIQEINRHKPDLGYAVKKMYAGKGEL"
     gene            27286..28074
                     /locus_tag="PLF66_05445"
     CDS             27286..28074
                     /locus_tag="PLF66_05445"
                     /inference="COORDINATES: protein motif:HMM:NF017946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HMZ63518.1"
                     /translation="MTYLKFISKAFQRSIAYKLEYYTGLMNAFLYIFIFTSVWQTVAK
                     ESPSSLGTWTGETLVSYAILSTLIKVSFGRNESLLTNKIKTGDIIYDILKPYNFVMMY
                     FADSFGVSLFQLFARAIPLLIFSILFFGIVPSVTAISLLQFIPVYILSFILFILFGFM
                     ISSLAFFFTEVFSFMILYSALVTLMSGSVIPLNLFPDYLVEIISWSPFPYLYYYPTTV
                     LIGTPIQMSYSELILRYLLELSIVGSLTFTAYFSGKRKLEFAGG"
     gene            28076..28918
                     /locus_tag="PLF66_05450"
     CDS             28076..28918
                     /locus_tag="PLF66_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002770975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HMZ63519.1"
                     /translation="MNLPHKLIKNENTFIETVYIYIRIATASIKSRMEYRASFLVFLF
                     TLLTFYAAQISTIGIVITRFKKIGGWSMGEMAFLYSLLILSQGIVTFIFSGILDFSTY
                     VRDGTFDRFLVRPLSPLSQVIMGGFEITGFAHIILGFLTFFMANTLITVYWNFTNAFI
                     FLAVVIGGALILGSIRIIIAAIAFYAVNTNSLVHLFVFSSREFLLYPLNIYSSGLKFL
                     LTFIVPLAFINFYPAHYFLNKTGENLFHPYFVFGTLPVGIIMIIFSLWFWRKGQDAYE
                     SAGG"
     gene            28971..29909
                     /gene="pyrB"
                     /locus_tag="PLF66_05455"
     CDS             28971..29909
                     /gene="pyrB"
                     /locus_tag="PLF66_05455"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002973015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HMZ63520.1"
                     /translation="MYQFPHKNILDTDQFSKEDLDFIIQKTRIMKNLLHSGKSFGMLH
                     GKLLASLFFEASTRTRLSFESAMERLGGRVISTVGFQFSSISKGETLYDTMKMVEAYA
                     DIAVIRHPVEGSSRIAAGAVKMPVINAGDGAGQHPTQALLDMYTIKSEKGKLDDITVG
                     FIGDLKYGRTIHSLINLLKHYKVHLYLISPEELALPDNYKKDVAGHSLTFEETRDIKK
                     IWNCDVIYVTRIQEERFPDHKEYDRLKETYKVNKELILASKKNTTVLHPLPRVNELST
                     DVDDLPNAAYFRQAEYGVIVRMTLLCLCLGIDINEA"
     gene            29912..30280
                     /locus_tag="PLF66_05460"
     CDS             29912..30280
                     /locus_tag="PLF66_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2203 domain-containing protein"
                     /protein_id="HMZ63521.1"
                     /translation="MFNPKKIWTLEEAREVFPQIKKITEGFHAKVESLVHLLTENIYP
                     ENEMESMEDEITDLLKAWNFTLQEFEVDVKGIWLVDFDNGNGYYCWKVGEEDLLYEHT
                     YEDGFAGRRLIERNKQNGDY"
     gene            30267..31493
                     /locus_tag="PLF66_05465"
     CDS             30267..31493
                     /locus_tag="PLF66_05465"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002103302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HMZ63522.1"
                     /translation="MAIINENYLKLKAGYLFPEIGKRVKKYSEENPDKKIIRLGIGDV
                     TLPLPPSIVKAMKDASDEMGTSSGFKGYGPEQGYGFLIDAIIQNDFASRGIKLSQDEV
                     FVSDGSKCDTGNIQEIFSIDSKIAVTDPVYPVYVDTNVMAGRTGAAGSDGRYANLIYL
                     PCTKDNNFEPELPKERPDLIYLCYPNNPTGTTITKEKLKTWVDYAHKNKSIILYDAAY
                     EAFITEGHLPKSIFEIDGAKEVAMEFRSFSKTAGFTGTRCAYIVIPKELMGYTGSGEK
                     VSINSLWSRRHTTKFNGVSYPIQKAAAACYSEQGKKEVKANIEYYMSNANLIRTSLQS
                     FGYEVFGGINAPYIWLKTPRNLNSWDFFDELLSKVQVVGTPGSGFGPSGEGFFRLSAF
                     GTRENVQEAMNRIKGL"
     gene            complement(31539..31802)
                     /locus_tag="PLF66_05470"
     CDS             complement(31539..31802)
                     /locus_tag="PLF66_05470"
                     /inference="COORDINATES: protein motif:HMM:NF017496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HMZ63523.1"
                     /translation="MSTLLEHPATARLVIEVSLTTYDLDFQKQFIYADALVMEYWLIN
                     LKEIEVEVYKEPNSGKYLEKKIDRSKDSIEIEGHQIQLKEFLG"
     gene            complement(31847..32938)
                     /locus_tag="PLF66_05475"
     CDS             complement(31847..32938)
                     /locus_tag="PLF66_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004501240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HMZ63524.1"
                     /translation="MKKIGIDVRMIEHSGIGVRILNLVEYLAKQNLSGFELYLFGERE
                     ILSKYGLDQKFPIIEYNTKVYSIKEFLGHPKMKEMDLLDIPHFNIPILYLKKCVVTIH
                     DIIPYKMKEFFPSKAKQIYIRLIFWLIRRFSRKIISVSECTKKDLIQEFHFSPEQIHN
                     IYNGLNQELFQPHKEKEVKEFRRKYNLEKDYLLTVGIGKGHKNLQFVIQSLIPLWLGG
                     NLDLPLILAGTSGKIPDHLQEMIQPVEKFILPFRKLSYEELPLLYQGAKMLIFPSLYE
                     GFGFPLLEAQGVGCPVFSSNASVMPEILGNSAFFFDPRNEKKFQEEFRLLMLNPSLLK
                     SKREQGYQNAKRFTWKKAAEDTVLLYKEV"
     gene            complement(32935..34326)
                     /gene="sthA"
                     /locus_tag="PLF66_05480"
     CDS             complement(32935..34326)
                     /gene="sthA"
                     /locus_tag="PLF66_05480"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (B-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HMZ63525.1"
                     /translation="MQNKKYDVIVIGTGPGGEGAAMKAVKAGKSVAVIEKYDRVGGGC
                     SHWGTIPSKSLRHAAQQLVDFKSNPLFEKYTNTIEYPHLVKTAESVINKQVKMRQSFY
                     ERNDIDIYYGHASYADAHTIEVRNNQHIIARLEADYFVIATGSRPYHPKDIDFTNPNV
                     FDSDTILKLNHTPYSITIYGAGVIGCEYASIFRSIGSKVNLVNTRDKLLAFLDDEIID
                     ALAYHLREQGILIRHNEEYSKVEFAANGVILHMKSGKQIKSDVFLFANGRTGNSKDLG
                     LDQIGIPVNSREQIDVNENYQTVLPHIYAVGDIIGYPSLASAAYDQGRFAATHIIEGK
                     CDFKLVHNIPTGIYTSPEISSIGKTEKELTDAKVPYEVGHALFKSLARAQITGRTVGM
                     LKLLFHRETLEILGIHCFGSEASEIVHIGQAIMTQKGDANNLLYFINTTFNYPTMAEA
                     YRVAALNGYNRLS"
     gene            complement(34375..34770)
                     /locus_tag="PLF66_05485"
     CDS             complement(34375..34770)
                     /locus_tag="PLF66_05485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63526.1"
                     /translation="MKRILFPLLLLLLLVFTYWQDLLYSPLTPKASAIAHFKISPKLL
                     AKQPPKNQSPYSSSASGPSEAKDTPVQNLTEEEIRAKHGRLELVRLIDNSFLKGVVIE
                     QGTDFIRVETNSGIKKIMTENVDTVEIIR"
     gene            35009..35929
                     /locus_tag="PLF66_05490"
     CDS             35009..35929
                     /locus_tag="PLF66_05490"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HMZ63527.1"
                     /translation="MNRKRFLTLATSLILIKKNWELTAEDTMREIKAIPMREIPSSRE
                     KIPVLGFGTWRVFDKDSTKENLITGMEVWEKFKEHGGKLIDSSPMYGRSEEFIGNLFE
                     KKKEENRFLATKVWTSGKKEGSHQINQSFQKMKTSFIDLFQIHNLLDWKTQMDTLREL
                     KAEGKIKYIGVTHYIPTYFKQIEEIIRKEKIDFIQIPYSISLRDAENSILPLAREKGI
                     AVLINRPFEGGELFSNFIKKPIDPRLKEFDIHSWAELFIRFLVANRDLTCILFASFNS
                     KHVEENMNAAKKPLLEKKDLEKVYKIFLEN"
     gene            complement(35932..36282)
                     /locus_tag="PLF66_05495"
     CDS             complement(35932..36282)
                     /locus_tag="PLF66_05495"
                     /inference="COORDINATES: protein motif:HMM:NF012708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HMZ63528.1"
                     /translation="MNLMFKIKLLLVKLFKRNILYIESGTSMIPLKILKRKTYLIGRG
                     VDPTKEIPVNSMELSRVHATIEKKKLDYYLIDNFSKNGTFLNGEKLAPGIPYILIHND
                     NIEMGDVTIKYLRE"
     gene            complement(36340..36858)
                     /locus_tag="PLF66_05500"
     CDS             complement(36340..36858)
                     /locus_tag="PLF66_05500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63529.1"
                     /translation="MSIFIYFYLLWDYTDTKVMNALILEKFFLINQLNLFKGTFVFLW
                     LIIVILINSCIIRLDWDRAGKPPRVYTYEHPHDGNLTRAEIARSGGEQPNGGGMYRIK
                     TEEELNGRPVHNCVSKTAKCLSDCNRNYDVFSTIFAPSVAIMHAKRASCYDECYNEIT
                     PGCSYPLKRIQY"
     gene            36845..37312
                     /locus_tag="PLF66_05505"
     CDS             36845..37312
                     /locus_tag="PLF66_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010413043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dps family protein"
                     /protein_id="HMZ63530.1"
                     /translation="MKIDIGISDAHRKNIAEGLKSLLSDTYMLYLKTHGYHWNVTGPM
                     FDSLHKMFMDQYTELWNAIDPIAERIRSLGFYAPGTYAEMSKLTSVKEDTKVPKAQDM
                     IKNTIIGHETVIRTAREIFDVAEKGKDQATMDLLTQRLDIHEKTAWMLRSLLE"
     gene            complement(37404..38657)
                     /gene="serS"
                     /locus_tag="PLF66_05510"
     CDS             complement(37404..38657)
                     /gene="serS"
                     /locus_tag="PLF66_05510"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008591194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HMZ63531.1"
                     /translation="MLDLNRIQSNPEELREMLKKRCMEDTSLVDRLNVIIQQKKKLQT
                     EVETLRAERNKVSKEIGIIKSKGGDIQELSNSMKDVGNKIKELEEALEKEETNLSDLN
                     LNLPNFLDPEVPFGKSEEDNVEVYKHGVKPEFSFAPKPHFEIGEALGIFDFEKGVKLA
                     GGRAYTYFGKAAKLERALMNFMLNTHGTEHGYEEVWVPMLVNDDSMKTTGQYPKFKDE
                     YYRLDQDGLSLIPTAEVPLTNLYRDEIIPEDKLPISIMAHTSCFRREAGSHGRDTRGL
                     VRVHQFQKVELVKFTKPEDSEAEHRKMLTNAENILKKLGLHYRVLLLCSKDTSNSSSK
                     TFDIEVWMPGLNRYMEISSVSNFKDFQARRGKIRYKSKEGKNQLVHTINGSGLAIGRT
                     LAAIVENYQTESGDFTIPDALQRYI"
     gene            complement(38659..39456)
                     /locus_tag="PLF66_05515"
     CDS             complement(38659..39456)
                     /locus_tag="PLF66_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012476477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HMZ63532.1"
                     /translation="MNYSLIDTHCHLDIIESQGQDIATSLEKSSSAGVKKIVQIGINL
                     DRSIAARKIALETKTNIELSYTIGCHPADNIDLPEIEDISKFIEENKDETSFVGIGEI
                     GLDYYHKTETKESQIKTFHHFMEMSVKYALPVIIHSRDAAEDTYQILKKYKDKAFGVI
                     HCFTYDTEYALKYAELGYYISFSGVVAFKNAADIQEAARKVPLESILIETDAPFLSPP
                     PHRGKRNDSSNLPFILEKMFSLRTEANHKVEEKIYENSVKFIHRKAV"
     gene            complement(39520..39978)
                     /locus_tag="PLF66_05520"
     CDS             complement(39520..39978)
                     /locus_tag="PLF66_05520"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HMZ63533.1"
                     /translation="MSLRNSFYVTIFVYLLTIFMILQKESFQTSEKIEIDKGSSKVFS
                     INFSQAEKGVGSVEEIKNNSSSEGSIAEEINQIRNKIVYPPQALEDGLESECEWSVTI
                     DVNQSATNIKTIHPCKYRIFEEEFYRVIHNWKFHLPPETILFIPVSFKIK"
     gene            40051..41943
                     /locus_tag="PLF66_05525"
     CDS             40051..41943
                     /locus_tag="PLF66_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002980311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMZ63534.1"
                     /translation="MKIFRRLLSYSLKYKYRFIAGIFFAVLTALINASSITVLIPLFD
                     NLGSEKKTNFRLELTAPEYNILIKEKIFGRNSLDGLEKLKRLIIVIKFRVNNTIKDMN
                     STEIVWMICKLIIPLYLLKMIAYLLSVICIATSGYNAVRDLRQELFDKVQTLPLTYFY
                     KEKTGLLMSRIINDAETVSAVISSNLRDAIINFFYVITHLFVLLYLNTELLLIASVTV
                     PIIIYPVTLFTKKISKSTSKFQERMADLNANVQEMISGIRVIRSFSMEDYELSKFNEI
                     NTKLSHRNFKGQFYLQVAPGLVELTSSIVVLGFFAVGAKLIFNGSFTQGEFMAFLLTL
                     LFLLRPLTQLSQMVGKVTQATISGDRIFEIIDKENAEAHDKAVGVHVNPIRNSIVFKD
                     IRFHYPETNTDVLNGINLDVKIGDTIAFVGASGSGKSTLMDLIPRFYNVTGGEITFDG
                     VNINDYNLHDLRRKIGIVTQNIFLFHGTVAENIAYGRPNSNLNEIIHAAKLANAHDFI
                     MEMEHGYSSVLGVRGLNLSGGQRQRLVIARAFLRNPEILILDEATSALDAHTEKLVGE
                     ALDTLFKNRTTFIIAHRLSTIRRIPKIVVLENGQIAEIGNHESLIAQNGLYAKLYESQ
                     FVPTQV"
     gene            41956..43263
                     /locus_tag="PLF66_05530"
     CDS             41956..43263
                     /locus_tag="PLF66_05530"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HMZ63535.1"
                     /translation="MKLINSKIISPWTLEFRDKDKEDAYIDSFIFDYARNSKLVIGIA
                     LVLGFIVDLSRDINILSAFLSNLNLFTFGVFFIFISYTKYFRKLFNPLTFSSNLIFSY
                     LAVTEVIKENGSSLEVLQIIICYTIFPRCYFKYSIIANAFTISFFSYLTWDIPIDSRN
                     TLSEVLFFLSITIYVELAAYLKEYANRNDYLTNQKIIDQEEKTNELLLNILPAKIAAE
                     LKKTGSVKPILYENVSILFTDFKGFTSIAEKMKPEELLKELDGFFFQFDEIAKRYNLE
                     KLKTIGDAYMCAGGIPKQNATHAVDTCLAALEIVELIKQIKEIKSSLNLPSWEIRIGI
                     HSGSVVGGVIGKTKFAFDIWGDTVNLASRMESSGEVARVNISEKTYHIVKDFFDCEYR
                     GELEAKNKGKVKMYFVNRIKPELSVDNNGRVPNDKFNLIYGSL"
     gene            43511..44023
                     /locus_tag="PLF66_05535"
     CDS             43511..44023
                     /locus_tag="PLF66_05535"
                     /inference="COORDINATES: protein motif:HMM:NF019235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1564 family protein"
                     /protein_id="HMZ63536.1"
                     /translation="MLQTFSQSTISFPRSNFQLSDFSSTLLIPEKYLSTLQLKLIQHK
                     GLRSYVAYLLSKYQLFIANGLIPGYSNVTTKYQDKGQNLQKFAFRPRPEDWAELKLYR
                     ISFGMSISAFFIYLLLADSVDLAKATSDYLEGVGISMIPNFDLSAKVFLWNKRTYYTT
                     LFHFRKRKEH"
     gene            44347..45078
                     /locus_tag="PLF66_05540"
     CDS             44347..45078
                     /locus_tag="PLF66_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020775842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HMZ63537.1"
                     /translation="MINPEIIKKIKTAKVVSVLTGAGISAESGIPTFRGAGGYWKNYK
                     AEELATPEAFARDPKLVWEWYAMRMKICAEAKPNAGHEIVAKMETYFPEFLLITQNVD
                     GLHKRAGNNKIAEIHGNIFTARCTACKKQFALNEIPSKFPVHCSFCKNLARPHIVWFG
                     ESYDSKLLQKVISFLERTDLILIIGTSGQVSVPIQLAMQAIDNGAYSIEINPENSTIS
                     NRADLHFSNPSGQILPNFWQEIIEN"
     gene            45146..46072
                     /locus_tag="PLF66_05545"
     CDS             45146..46072
                     /locus_tag="PLF66_05545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63538.1"
                     /translation="MRSYTKSIFIIFLVSFLSAIHAKPSEDKGIKKAIRKGDTEFFKN
                     IKFEDLKEGNKPNSEGKTLITRMEIDGVNALVGAFKNPDVEVRKIIYKELLYPEIFKD
                     LEGVTVAEFMEQDKQLKQYLDKLRVAIYSVDELENPEANESLNALIKQYGYSVKEAIK
                     VKNPKALAIMSPDFFKKSNLEDPKTVIVRFKKNGVKTLVKALEYEKGNPETRKLIIES
                     LFYKTLGGPEKVVTKKKTRESRGENLFLIADKEKNPEVRAQLIQLAEAYYFVEDEATE
                     SSVEKKTENAKTESSKTKEDDEEEEEIKEAKK"
     gene            46114..47496
                     /locus_tag="PLF66_05550"
     CDS             46114..47496
                     /locus_tag="PLF66_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002180473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HMZ63539.1"
                     /translation="MNYPNNFKKGFLSYIGLGELAAQGSRAILAFWLIICMTFFLFGD
                     QNLIAPNLKNIANSFGIIDQKDIDWYIGGLIPIFFFILGGLISISMGYFSQKFSRKNL
                     LIFSVLMGEIPCFLTGFSNSYTEFFIYRVLCGFGLGGVFPILFSIVGDYFSIRSRVTA
                     TAYISLAMGLGVGIGQLVGGILGNSDPINGWRTSFIAMSAPAFLFVLVYAIFCKEPKR
                     GIMEDEFKDINVEELNNHLSWQDVKVILKSKTNLGVFLQGIPGCVPWGVFFVFLVDYY
                     ESTYLLSKAEASGLLTFAAIGIFLGTFLGGVIGQRLFNINHIYQPIFAMTSIFLGVFP
                     CLYLLHADSIARSALFIPMNIFAGMIITFPLSNVRSILINANAPKNRSAAFALYNLTD
                     DLGKGLGPALSAIILNLIPDRTMALSISILFWIPCAIFWLPIVFNFKKDEMNVRETLL
                     KDAQRIKVTK"
     gene            47493..48218
                     /gene="mtnC"
                     /locus_tag="PLF66_05555"
     CDS             47493..48218
                     /gene="mtnC"
                     /locus_tag="PLF66_05555"
                     /EC_number="3.1.3.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017808182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_function: GO:0016862 - intramolecular oxidoreductase
                     activity, interconverting keto- and enol-groups [Evidence
                     IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acireductone synthase"
                     /protein_id="HMZ63540.1"
                     /translation="MKLENYSACLFDIEGTTTPISFVHDKLFPYSRDKMESFLLSQKL
                     EPHIITELIEENKKDFSEGTFSAPILGSDHVVNLQILVSYLHYLIRVDRKSKPLKEIQ
                     GKIWKTGYEKGELKSEIFADVLPFFKKLHERKIPIAIYSSGSIEAQKYIFQYSNYGNL
                     TKYISYYFDTNIGNKRESKSYVEIARQIGINPNRVLFFTDIKEEANACHQVGVSAIIL
                     DRPGNHLQAQHNFPIITDFYEFI"
     gene            48205..49209
                     /locus_tag="PLF66_05560"
     CDS             48205..49209
                     /locus_tag="PLF66_05560"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HMZ63541.1"
                     /translation="MSSYNTPENSTALNQFLNYKGEDFQKLILIPKEASIRNYYRVIY
                     ENQELVLCIDENFKTIPYPFLDIRNFLEENSIPVPKILNYDPNLNAILMNDAGEKDLT
                     SILDDSEYISQLKISLEYILKLQTLNPIPIIADRSFHYEKLMFEVNHTCSGYLRFKEK
                     YKIELDITSEMYAFLQEASGFLAKFEEKVICHRDYHARNILLNIKHEQTIIDFQDMMM
                     GTPQYDLASLVYDAYRPLSIQRREELYSFFKERSQFKNKRFREYYLTQALQRSFKALG
                     TYLVQFNEKQNLKYKESIPKALENLMEICQLGRFPDQLYVFFYLLNKRLTENTLFNES
                     "
     gene            complement(49206..49823)
                     /locus_tag="PLF66_05565"
     CDS             complement(49206..49823)
                     /locus_tag="PLF66_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015682015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HMZ63542.1"
                     /translation="MIVDEKKETPSLEHKPRRSKRAIVHRFIDRNRLNVGLSISTVFQ
                     LLILFFWYTPDLKFSSLDKFVDEVAFVDSVSITENVADTPNDGDVELTDQLKKENKED
                     PRIAGAQDAILSGATAPVDLSPTEKPEYTEEARSAGVEGAMTLEVVIADTGEVLQVRS
                     VGKKFGFGLEEEAIKVYRSKKFSPSFLDGKAITVKVLIPIRFKLN"
     gene            complement(49834..50256)
                     /locus_tag="PLF66_05570"
     CDS             complement(49834..50256)
                     /locus_tag="PLF66_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002972391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HMZ63543.1"
                     /translation="MIKRNKSTPSIPVSSMADIAFLLLVFFMVTSVLDSDPDLPISLP
                     DVPGGEQLNKKVSNIFLSADKDRTVYYNSMRVPLAEAINNVRAKLATTPDLKVLIHAD
                     KDLSYGEVDHVFDMLKEAGALKISLVTQTTQGGGLKGQ"
     gene            complement(50253..50672)
                     /locus_tag="PLF66_05575"
     CDS             complement(50253..50672)
                     /locus_tag="PLF66_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016762370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HMZ63544.1"
                     /translation="MVKLRKKVEMEEISAASMSDIAFLLLVFFMVTAVFFVKEGLNIQ
                     LPRKNVPPTQVLRSNVFEINVSGDTVSMRNVSVGTKYYKSLKEFREELNQLEIQDIST
                     KLAIISTSGETKYGNMLDALSAVQLKGFTQVSVRKKK"
     gene            complement(50679..51518)
                     /locus_tag="PLF66_05580"
     CDS             complement(50679..51518)
                     /locus_tag="PLF66_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015681534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HMZ63545.1"
                     /translation="MKLLIPKTAIKSIAFTTILLTILYLFAPIFAQSTTEQPTTPTQV
                     ESKDSPKTNDPSLGTEEKAKAPTKSPGLVDLFIAGGWSMWPLALSSIIGFGIALERLY
                     FFFTTKLVSKGYNQILSDKIEEKGLEGALSHLKENGEQRISQILSDGVDVSSGDPDLF
                     AKGVEREAVEVITLLERGLTILASVSTIAPLIGFLGTVSGMINAFDAIANADQVNAKV
                     VAGGIKEALITTAAGLIVAIPAMSFYQFLSGKVNGFAAEVEEGANKIYKELLRKKGAL
                     IAK"
     gene            51843..52388
                     /locus_tag="PLF66_05585"
     CDS             51843..52388
                     /locus_tag="PLF66_05585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63546.1"
                     /translation="MEQVRKITETILEERNLNKFFAFASTVIKEFLQNGAKATQKRIF
                     FKNHGLDIDKNHKQGIEKFKESMSNGDIHNHQLIDGIDFLVELRLQELDKGVLISVRN
                     HGEMNENEIKNVNFMIDRGRRTNEVSDLLSDETSNKEGGGLGLSMIVILMKKLGLTPD
                     CLYYEVKNSFTSFNVKIPYNI"
     gene            52407..52733
                     /locus_tag="PLF66_05590"
     CDS             52407..52733
                     /locus_tag="PLF66_05590"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HMZ63547.1"
                     /translation="MELKVKDHNKTKIFQLVGSLDIYTASKVKKEITQIIDDEEAESV
                     VLDMSNVNHMDSSGIALLANIQKKMKSSGNKFGLLAPTNDIMAVLKLSSLDSFFTIYN
                     TEAEIR"
     gene            complement(52751..53605)
                     /locus_tag="PLF66_05595"
     CDS             complement(52751..53605)
                     /locus_tag="PLF66_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016547943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HMZ63548.1"
                     /translation="MKDPKEITHNSSKEERDYVLEYQKIDITQKERISLLGVEIDNIT
                     RDEAIAHILDFHKKKDSFHHILFIDPIKLMLMQPGKKMNRIARKASLVIAEGGGIEWA
                     ANKLGFKLKERISIISLLMDLIRYSEKKELTLFFLGSKEDIIERLFFNLIRHFPEIRI
                     VGRHSGYLNDARELMVKEAIRKTNPDIIIIGMDFPRQEVWIENNTGYFGKSIVIGVWG
                     NLDTLSGQIKKAPDYFQLRGLTWLWRIFVRPYRLDKIYNTIQFYSSILLENWKLKREA
                     NNKIKSVE"
     gene            complement(53602..54156)
                     /locus_tag="PLF66_05600"
     CDS             complement(53602..54156)
                     /locus_tag="PLF66_05600"
                     /inference="COORDINATES: protein motif:HMM:NF014622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HMZ63549.1"
                     /translation="MFFFQLRAKSLNENEYLKLYLEVSRFFPDVRIIVNDFWEIGIKE
                     SSFGIHIGKEDYIRLSDSEKRVLKENKSILKGTSSHNLEDILSLDTEIWDYTGFGPMF
                     KTETKKSHYPTLGIEELKKALFKTRIPLVPIGGIDLSNFFIVYEVGNILPASIGMLSN
                     EKSLVKIVDFIRNSHHPVRESKPV"
     gene            complement(54282..54479)
                     /gene="thiS"
                     /locus_tag="PLF66_05605"
     CDS             complement(54282..54479)
                     /gene="thiS"
                     /locus_tag="PLF66_05605"
                     /inference="COORDINATES: protein motif:HMM:TIGR01683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HMZ63550.1"
                     /translation="MKVNGKQVDISGNSFTISEYLTSIRLNPNTIAIEWNGEILEREK
                     WQSTYIKDSDTLEIIKFVGGG"
     gene            54535..54843
                     /locus_tag="PLF66_05610"
     CDS             54535..54843
                     /locus_tag="PLF66_05610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63551.1"
                     /translation="MNRFVKFLFILLIPFIFIHCPTHPSNPDKTTRNAVTLYYFNCKG
                     GSLAACNSGCNPQCGIADGAAVTTDKFSCLTNCQSTCSSDCNITTTLLLYLSNHPIKK
                     "
     gene            54880..55269
                     /locus_tag="PLF66_05615"
     CDS             54880..55269
                     /locus_tag="PLF66_05615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63552.1"
                     /translation="MSLITCSRSNDSKNNNALIISLLYNNYKNSQSDYNCKTSPSPLS
                     FADFRTAIDTLNTSGYKCSECHGANTAQANFIITNYSSVGERVLPGNPKGSILYFKVQ
                     PGGSMNAYSNANVRQAIYCWIANGANP"
     gene            complement(55276..55737)
                     /locus_tag="PLF66_05620"
     CDS             complement(55276..55737)
                     /locus_tag="PLF66_05620"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011670435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HMZ63553.1"
                     /translation="MTKAIFQTNHGSFTLELAMDKAPITAGNFAKLAKEGFYNGLTFH
                     RIIKNFMIQGGCPKGNGTGGPGYKIKDEFHPDLKNAKFTISMANAGPNTGGSQFFINV
                     KDNYYLDNRHAVFGHVIDGSDLVLKISEVKTGFQDSPVEKVIMETVTIVEE"
BASE COUNT        18920 a         9471 c         8909 g        18672 t
ORIGIN      
        1 atattctttc ttttgtacaa tccttatttt aggcgacttg acaaagtcgc attaatttcc
       61 caaatttaat aataggagcc caaacaatga cgaatgtttt ctcaaatgca aaactctcat
      121 tcccaagaat gaatttttct ctaaatgatt ttaattcaac ccttctcatt ccggaaaaat
      181 atcagtcctt attcataaaa aaaatagaaa aacataaagg cattcgaagt tatgtcgcat
      241 accttctatc caaataccaa gtattaatca agaacggtct gattcccggt tattctaatg
      301 taacaaccaa ataccaggaa aaaggtcaaa atctaagaaa atttgcattt cgccctagac
      361 ccgaagattg ggcagaactc aagctctatc gtgttagttt tggtatgtct atctctgcct
      421 tctttgtata cctactaata gctgattcag tagattttgc agagaccatc tcggactacc
      481 taacgaccgt aggaatttca ttggtctcga atttaaactt gaccgcaaag gtgcatcttt
      541 ggaatcaaaa gaccttctac actaaagttt tacaataccg aaagagcaaa tatccctgaa
      601 gtaatagaaa atttttcaaa ctaaaaacaa agtaagatta atagatcgag gaaactatcg
      661 tctattatac ttcattcttt gaatttcttc aaactctgtt ttcatttgat ctgcctttgc
      721 gggggtagga aaggatactt gtaagttctt atcgaattca agtttatagg aatactcgaa
      781 agtttttgct ttcgttgcag agctatctgc ttgtagacca agatcccagc gcaaaatacc
      841 tcttatccgc tcatactgct gataaagttc gtcggaactg agtgctgagt tttttaggtc
      901 taaggtgatt cgaacatttt ctttttcatc ttctgcgggg atacgatcaa ggacacgaag
      961 gtcggttggt tttttagaaa aattttcgag tatcattctg attttaaaat ttaattcttt
     1021 gtttccaccc aaaattttct ctttcttctc cacgagttct cttctagctc tgatttgagt
     1081 atctatacca aatcccatta cgaaggattg tcctttagca acattagaaa tttcgccatg
     1141 acctacaaat ttttcatcta ggtaaacact gacaggacct tctaaaaggt tttcaaaaat
     1201 ttcattttcg atttcagctt ctctataaac ataggaggtt agaaggggaa tagctacatt
     1261 gtaaaattta gcggataatt ctgatttatc gacttttaca agttgttgtt cattcttact
     1321 gctaaaactt acttttgatt ttaaattaaa actaacgcta ggagaggcat tgttttgttt
     1381 ggaatctttt tttagaacat ttaaatcttc atcttttgcg actaattcca agttttgata
     1441 ttcattagcc gcactattca tcgcccaatt tccctcttgg gtttcattcc aattttgagc
     1501 accaatttgt ttattataag cggcactcat ttttttagag atgccttgtg aagcagactt
     1561 cagatcatct aggcctattg aatttcctaa agtagaaaga ctaatcctaa aaggagagag
     1621 accgggagca agagcgctta acgcaggagt tgcattcgaa agagttaaat ttacattttc
     1681 ccaattttca ccagtgattt gctggatacg agcattaaat tctaccttaa cagttttaga
     1741 tccaagtttt gaatagaaat tataaatagg agaccagcca gcgttttgga ctaaatagaa
     1801 taatttgatt tcgcatttcc cgtttccaat tttatctaag aagatacttg catccatact
     1861 tttaagagaa gttttaacaa gttgttttct ttctctagcc agaagatccc tctctctatt
     1921 taatgcttta atatcctctt gaatttttat ttcttctaat gcaagatctc tcctttgttc
     1981 aaaatgaaat aacgtcagtt gctttatcgt ctctgcattt aaaagacctc tatttaattc
     2041 aattttctct gatgttactg taaaatcttc ctgttttgtc aagaatgcta acttgagtcg
     2101 attcacttca gaaagtttag agagtttaat aaattcttga tttaattttt caattttatc
     2161 atctaaggca gcaagttttg gatcagaatc tatttttact tctgtaatct ttaattttgc
     2221 agctcttact tccgctccaa ttgcttctgc atataaacta ttctgaatca attcagaagg
     2281 aagttttgaa attgtaattt catgtgatcc ctccttcaaa tctaattcaa tgaccctagt
     2341 tacgagtgct tggtttcgat aaagaaccac atcttgaatc tttccttctt tggaaatagg
     2401 ttccgccaat aaattaatgc aaattaaaat aaaaaaaacg aaaatacttt tcttatacat
     2461 gggattctcc taatcagata aatcgcttaa cgcatattta aactaaactt aaataattac
     2521 taatttgaat agatagatgt atcaatggta aaaataagag gcacatgatc actcatatca
     2581 atccattttt gaaatggttc aatttttaaa tcaatagatt tttttaaaaa attttctgag
     2641 gcaaacacat aatcaatatg ataccccttt tctttctttt tatgaagaaa aaaagtctta
     2701 gttttttcat ttccttgctt ttcatatgtt agtttgtgat aggctgaaaa aatatttaat
     2761 gcttctaact ctctaacaca gtcagaatga ttccaccaaa tatcccattt atcccaaatc
     2821 gaattgctat taaagtcacc gcagataatt atattcttaa attctttctt attattctga
     2881 agaaaaagcc aaaattgccc aatgtaggaa aatacttttg catccgcatg atgattccaa
     2941 acagcaagaa ttcggaattc tttgttcact tgaaaaggta gaaaatattt taaggttctc
     3001 cctctataag aaaaattcca atctagtttt tctatttcta tattcttttt agaaaacaca
     3061 cctaaacctt tgtttttatt ttccccagtc caaagaaaat tttctataga attttctaag
     3121 agttcttttt gaaaagattc atattttcct atctcttcac attcttggat aatgtaaatg
     3181 tccgcattga agcctaataa tctcttaaat ttcttacgaa agcccccatt gcaattccaa
     3241 gtgattatct tcatggactc tttttacaaa cgactaatgc ttatttaccg cagatgaaag
     3301 gaaaaccaat gagcttgccc cgattctagt ttgataaaca ttcctctttc tatcatttct
     3361 ttttctctag agtaagtttc tccggtcaga acatctttta aaataatctc atcttgtttg
     3421 tattgaagca ctttttctcg aatctgttct ggtagatgta acgctcctat gacttgatga
     3481 ggatatgtgt taaaaatcag aatctcaact aggttgtcat ctagatttaa aacagatcga
     3541 acaatacaat ttttgagtga atagtcatcc atatatgctt ttagttcaaa tgtttctaat
     3601 tgcaaatttt tattctttct tttttgaatt ctataaaatg ctctatcata gaaattttcg
     3661 ctctcaagat ttatttcttc ctttggtcca cgagcgagtt gcacaggaat ttttatttgt
     3721 ttaccctgtc tttgtccctg gtgatacaat acacttccag gtaaaaaaca aagtagcgca
     3781 aaacaagaat tagcccaact tttaaaagta gagacagctc tttcctcatc atgattttca
     3841 ataaaccgaa gggaatggct ttgatactcc tccgttgctt ccaaatgaag tcgaattcct
     3901 tctgcctctc gactcttcaa cctatcatac aacgtcttat cataggtgta atcaaaacca
     3961 agttgttgca aatcatattc tcgattccaa tatacttctg ctataaataa aaaatttgga
     4021 tatctttctt ttactgaatc gatcaattct ttccaataag gtagtgcacg tatcccatgt
     4081 gttttttcga aaacatcttc gagtggtagc atcgccatgt cgcatcgaac tccatcacaa
     4141 acttccgaaa tattggataa aattttctta tgaagtttga ttgtatctgg atgagagaaa
     4201 tcccattgca ctgtatctgt ccaaccatca taataaggat cccttccata aaaatagatt
     4261 cttccatttt tgtgttgaaa agaattttta caaacctctg cattctcttc ttttttatat
     4321 aaaaataaat ctggatattt atcaatcaaa ggggaatcta cagccatatg gtttggcaca
     4381 aaatctagaa ttaatttttt cccaattgag tttagtttgt atttaaactg tttcaactct
     4441 aaaaaatgtt cagcaatcaa aggatttggt tcatatttga aaattgcata aggtgaacca
     4501 ataatgtcct ttgtctccaa atcagaaaga gtttttctaa attcatgatt gagtccctca
     4561 tgttcactgc aaatttgtat cgacttttga gaaggagacc aaacccccat aagccaaata
     4621 gcatcagacg caaatacttc cggggaacga aagaaatttt cgggggcacc aagcaccctt
     4681 actccattct caatacaaaa aattcttgta tttaattgat aaataaaaag ttgattaaag
     4741 taattatgat tcataaggat actcgattga aaccaaaaaa tattttcatt acaaacataa
     4801 tcattttaag tttattaatt catccattct tttcttgtaa aaaaacagaa aaagtagaag
     4861 aaattcaaag tatcaaagta gaggacattc catggaatgg agatcaaaac tcaatcccag
     4921 aaccgttaag acagcctaat cctgttgctt cgcccaatgc agtgaaagga ggagcattta
     4981 aaatctacag ccatcagttt ccaaagtctc tcaattacta cttagaacaa ttttctacaa
     5041 cggctcgtat ttatacttct atgtttgagc cactcacaat gaatcatcca atcacattgg
     5101 aaaatattcc gcatatcgct aaagaatgga aaatttctaa agataaaaag aaatttacat
     5161 tcatcataga ccaaaacgca aaatggtctg atgataaacc gattactgca cacgatgttc
     5221 tttttacatt caatacgatc atggataaaa aaaataacac tgccgtattt cgtattggtc
     5281 ttgctcgatt tgaggaacca gtggtgatcg atgattatac gatagaattc accgcaaaaa
     5341 atattcattg ggacaatttt aatacaattg caactggaat ttatatttta ccaaagcatt
     5401 attatgaagg aaaagatttt aacaaagaaa acttcgaatt tccagttgta tccggtcctt
     5461 ataaattagc agaatccaag ccaggacgtt atgttagaat ggttcgccgt ggtgactact
     5521 ggcaaagagc ctatcctttt aacaaaggca gatacaactt cgatgatgta ttttacaaag
     5581 tatacaacga agaagcaatt ggatttcaag caatgctcaa aggggatatg gatattttcc
     5641 cttcttacaa agcagctact tgggtaacag aagctgttgg agaaaagttt gataaaaatt
     5701 acattgtaaa gcaaagaatt ttcaatgaga agccactcgg atttcaagga tgggcgatga
     5761 actcacgccg tgatattttt aaagataaaa gagtccgcaa agcaattgct catttagtag
     5821 atcgaaagca aatgattgaa aaacttgctt acaatgaata tgatccgaca aacagctatt
     5881 atccagactt ttatctaaat ggtgaagtaa atccaaatga gccagtcgag tatgatccag
     5941 aaaaagcaag agagctttta aaagaagctg gctggaaagc taacgcaaat ggaattttag
     6001 aaaaagatgg aaaagagttt tcgattacaa tactagatcg tgataaaagc actgaaaaat
     6061 atttcacagt ttttatggaa gcagctaaac aacttggaat caaagcaaat atcgaaacaa
     6121 cagatatggc agcttggagt gcaagaaaag acaattatga ttttgattta acttgggcag
     6181 cttggggtgg tggagttttt aaagatcctg aaccaatgtg gttttcaaaa tatgcagatg
     6241 aaaaaggaca aaacaaccta gctggaatta aaaacaagga agtagatgcg ttaatcgaaa
     6301 aacaaaaatc agaatttagt ttaaaaaaga gaaatgatat cgttaaaaaa attgatacga
     6361 ttatttataa agagtatcct tatgttttac tttggcattt aacgaatgtt cgtcttatgt
     6421 attggaataa atttggaatg cctgaaatgc ctcttggaag atatggaacc gaagattatg
     6481 caactgacta ctggtggttt gatgcaaaga aaaatgagga acttgaaaac gctattaaaa
     6541 ataacactgc tttaccagaa agtccgaaag aagtaaaact tccaaagttt gatcctcctc
     6601 ctggattaga agaagaaaca gaaaccgagt tacctcctaa gaaagtagaa ccagctaaaa
     6661 aagaggtaaa gcaaaccaaa tccaaaaaag cagacacaaa gaagaaaact acagataaaa
     6721 aaaagaaata agacatgtca gatattaaaa cgatagttga tacagataga actgatttca
     6781 taaaaaaaga aattttacat tggaacagtt ttatcccaga acaagatcaa tttaaaaaaa
     6841 aattacaaga tatgaaagaa tcattctttc atttctttag agccacgaat catttatttt
     6901 ataaagacat tgattctggc ttaattcaaa ttccaactga atggaaagaa actccccgta
     6961 tccaaacatg gatacaggga gatttacata cacaaaatct aggcttcttt gaaaatgcaa
     7021 aaggtgaaat aaagttagat gtaaatgact ttgatgaatc ttatatagct cctttttatt
     7081 tcgatttaat tcgctttgta actagtattt ttttacagaa agaaagcata aatttcaata
     7141 tgagtcacaa agaagcagag gaattagccg aacatttctt agaagagtat caagagacct
     7201 tagaagaaat cagtgatgag ggaagagaat tagaactcat cgaaagcaat ctagatggat
     7261 ttccaaaaga tacattagaa gaactacgcg acaaaaaatc taattccact ttactagaca
     7321 aatggactaa agtaacaaat ggagttagag aattcgaatt ttcaaatcca gatttaactc
     7381 aattactaga tgaagagcat agagaaataa cttccttttg gtatacctat ttagaaagct
     7441 taagtgaatt cgcacaagaa agaggtagca atttttttca aatcttgaag ttagcaagac
     7501 gattgaattc aggtcttgga agtcaaggag tttacagata ttatgcgctc attcaagcag
     7561 atggaaatcc tttattacta gaaattaaag aacaaagact accgtcttta ttcctactaa
     7621 aagacttatc tgaagctgaa tacaatacat ttttcccaaa tcatgcagaa agaacaaaga
     7681 ctgcatttag aactatggtt gcaaatcctg atggctttct tggcactctt tctacatcca
     7741 agcgatctta tttagtcact cgtatttcac cttataaaaa gaaactagaa tcgaaagact
     7801 ttcattcaga atctgatttc aaaaaatacc taaaacattc agcaagagcg atcgcatatg
     7861 cacatagtcg cgcttataaa tctattttaa taaaatcaac atccctatct ttcgcaaatt
     7921 ctgcgttatc cgcaataaaa gcttgggcag gaacaaaaca aactataatg aatttagggg
     7981 aaaagtattc tgaacaggtc aaatctgatt atgatagctt tttaaaaagt ttttaattaa
     8041 tgacagagct aaaacaagaa atagatttat ttttacgaag aagctttctc gaccctttta
     8101 aaagaagact agtgatcaat tccaaattga ttcaatatga aaagaaatcc attccttgta
     8161 gtgaaatacg acaagttcgt tatggaatct tacagatgta tataaacggt attaaagcaa
     8221 atcggattta cgaaatagga cttaccacaa ataatttcaa gcctattcga atcacatttc
     8281 aatcttctag aatctttttt aaaaatgaaa agttagaaaa tacatataat gatataatag
     8341 aatgtctttg gaatcaggtc actaaaagac ttgtaacgga agcaattgaa aatctagaaa
     8401 aaggcaaaac ctttagtatg gaaaaaattg aagttcatcc aaagggaatt caaatgcaag
     8461 taagaagttg gctttttaaa aagaatattt actttgtcga atggaaagat gtaagaaaat
     8521 attcacaaga tggacttctt tgtatttatt cagagcaaaa caaaaaagca aagattaact
     8581 taaactatca aaaggattgg aatacgcctg tattatcctc tcttcttgac tacctctggg
     8641 aaaatgggcg cgcatatgca ctctccgaag atcgcttcta aatgaatcga agaaaatagt
     8701 aattatccgc tagagaggaa tcaaaagttt tgaatctaaa cacagctact tgacaaaaat
     8761 agaagcagat aggattttaa tttaatctat aaattaatct ttgaggcaaa caatgagtaa
     8821 actatttcta tatactcccc gaatccttgc aggcattatg atgctattat ttggactaga
     8881 taaactattt cccttcatgc cacatcctcc agctgcagaa gcaggaatac tttttaagag
     8941 tgcgttagta gctacaggat atttttttaa atttgtcgga ctttgcgaaa tctttgtagg
     9001 actcgcattt atttcaggct attttgttcc tattgctctt ttggtaatgg ctcctctttg
     9061 tgtaaatttt attttatatc atatttttct cgatccggca aatatagccg gagcattatt
     9121 tgtgactgcc gtctgtcttt ttttaacatg gcaaaattgg gaaattttta agccattggt
     9181 aaagcctaga taattcattg acttgcggat tggagctaga atcataacat ttgagtttta
     9241 ttttgaaact ctaggagtat ttatgctacc accacttgca atatttaatc taggtccatg
     9301 ggaaattacc tttatactac ttttagcttt attacttttc ggaggaaaaa aactgccttc
     9361 tcttgctaag gatttaggaa ctggaattaa agaatttaaa aaatctcttt caagtgcatc
     9421 aacagaagac atcgaaccac cacaacctcc agtcactccg gttcaagaag aaccaactaa
     9481 aaaaaaggca ggaaagaaat cttaaactag atggcttcca attcaaaaac gccaaagaaa
     9541 aggccgtcta ccaaaaaaaa caaaccaatc cttgaggctc cagtagcact caaggaaatt
     9601 ccaatgactc ttccagacaa acaaacagaa gaagtcattc caaatgatcg cgaaaaatat
     9661 atgagtctcg gcgatcactt agaggaatta agacaacggc taatcaagtg tttgctcatt
     9721 gtaggactat ttacaattgt atttatgatc tttggttctg aagtgcattc aatctttgca
     9781 tctccttaca aaaaagttct cggtgaaaag gcaacatttt accaagtaaa gttaatggct
     9841 ccaatgttga tctatttaaa gacatccttt atattagcca ttttatttag tgttccttta
     9901 ttactttata ttatctgggg atttatttct ccagctgttg atatagaaat ggaacgttat
     9961 gggaaactta tgattgtatt tgcgactcta ctgttctgga ctggacttgc cgtttgctgg
    10021 tttactgttt tcgaaaattt tctcagggtt ttccttgtca tgtttcaacc aggagatatt
    10081 gatacaaaac ttccaattga tgaatactac gatgtttttt tcaatctaca tctaatcttt
    10141 ggaatcagct tcgagttgcc tgtagttctg attctactgg gaagaattgg tcttattagt
    10201 tctgatttct tactcaataa atggagagaa acaacagtta tactttccgt attttccgcg
    10261 gtgctttctc ctggaccaga tgtgttttct atgatgatgc tttttgctcc tcttttagtt
    10321 ttgttttttt tatctatcat aattatgcgt atagctgaaa cccgaaaaga gagttaggtt
    10381 tggtttgaca taggacttaa atcttaaatt ctgttaaaca atgaatatta tttcgcttat
    10441 aaccttccgc tatattcgtg gatcccgcgt ttttggctta ttatcactta aatctagact
    10501 ttcttttatt gttatgatgg tcggagtttc cctcctgatt gttgtattat ctatattcaa
    10561 tggatttcaa aaacaagtaa aagagtcttt atggagtggc ggaccccata tcacaattga
    10621 aaatacacag ggtagtggcg atatccaaaa atatgagaga atgatagaac tcctcttaca
    10681 aaaccctgag ataaaagagc gagttatttc tatgcaaggc aacattacaa gtcatggact
    10741 tttacagaac aataatacat ttgttcctgt tatgattcgc gcagttcctg ttccaaacga
    10801 agaagaattg gtagaaaaca aagttccgaa ctttccaaag ttagattatt acaatagaga
    10861 tgaagtaaaa gatctaaact ctaaaaatct tgtagtgatc gggaaggaaa tgtccctact
    10921 ttatcattat aatctaggac gaaatattac attggcagtt cctagcggaa gttttaacgt
    10981 tagtcgaggt gtcgaattaa gcatttctaa ttttgcggta accggtctat ttaaaacagg
    11041 ttattataac tatgactcta aattcatttt tatgtcttta cctaccgcac agaaattttt
    11101 caaaatgaaa aattcagtaa accaaattac agtaaaagta aaatcactcg atgatttgca
    11161 actatgcaaa caattaattt tgcaaacaat tagctcatct gattttgaca gtagtattac
    11221 agaagggacc tatttttcag taagaacaat tgcagaagag caagctaact tccttggtgc
    11281 acttagaatg gaaaagacta tcatttcgaa tatcgtcttt ttatttatag ttctcgcagc
    11341 acttggtatg gttgccagtg tatattccct tgttcgttcc aaaagaaaat ctatcggaac
    11401 tttaaaagca cttggacttc catcctcttc tatactttta atctttactt taaattccat
    11461 gattattgga ttattcgctt ccatcattgg aggaatcatt ggtatttatt atgcaaataa
    11521 tctggaatca attattgatg ggttaagtga agtaatcaat acattcggta gagcttttag
    11581 taaaggggaa tggcatgaga ttaagcttgt tccgaaagat atttattatt ttgatcatct
    11641 acctgtggat atagacatct cttttatctt tatggtaaca acagcagcaa cgattttatc
    11701 tggtttagct ggttactttc ctgctagatg ggcagcgaat ttagatccag tagaaacaat
    11761 aaaaaatgat taaacagaaa cggctaatac atattagccg ctttttaact aagatataat
    11821 actgtaatta ctctctattg aaatgtaaag ctcaaaccat agtttttttc taagcagttt
    11881 ttttaaaatc actagactat aaagttaaga gaagaatatg attataggga aaacatattc
    11941 tgattctata aaataaaact tatatgagta tagaatgcgt aaaaaaaaac aaacagcagt
    12001 atattttaaa aataaactcc agtttggcat cgagaatggt tagcttaaaa tattaaagct
    12061 ataggaggta gaatgaaagg tgctatacac aattacttaa tagactattt caaaagatta
    12121 ggtctatctg aagattctct ttcaggtaaa acgaagatca gatcaattcg aattcaaatt
    12181 tctttgaaag gattaaattt agcaaattat ggtccagata caattgacaa tccagttgaa
    12241 ttaaataaat taaaatcaat attagaaagt ttaaaattag gagcagctta gtgaattctc
    12301 ttataacgtc atctatatat aaaaattcaa tcgatctatt tacagctata gaaaatagaa
    12361 atgttggcct cattatatgt ttttatggtt ctgattataa ttatacagaa ctttatcata
    12421 acttgaaaaa aaccaaaata ccttttattg gttgtatgga cattgccaga tttgcaaatg
    12481 ctagttatca atttgaaaca gattctgtcg cagtcatgac aatttcaaat gaaattcttg
    12541 aaaaagtatc cataattgct tacgacatgc gcaaaacaac gagtaaccac tcaatgtatc
    12601 aaactagtaa agatgaatac tctagagctc taaacacact tggtttcgac ttacaatcac
    12661 cagatatgga aagagaattt tctattaatc ttttatatgg actgcaatca gctaactctg
    12721 tattatccgc tcaaaatgaa gtcagtcttt ttttacaaac tgtaggtgga agttccggtg
    12781 gaaaattgga tttcaaaagt tcaagcgtaa tctgcagcaa aggaatgggg tctatcgggg
    12841 caacagctat cgttagattg aagcccgagt attcctttat gattgatcga acttccagtt
    12901 ttgattcaca tgaaggaaag ctagtcgtga caaaattagc aaacccaaga tatatccttg
    12961 agttcaatgg aaaacctgcc gcaatggaat atgcacgtgt gattggtaaa aatgtttcag
    13021 atctaaatcc tgagatatat gctctttata cactaggagt tgaaccgggt gatggagaaa
    13081 gacttattac tagcatcatg aaagatgatg gaaataacgg tcttttaaca tacaatgact
    13141 tactagaagg aacagaatta aatatctata aagcaaaaac acaatttgaa gaccgtgcag
    13201 caaaactcaa aatgttagat caaaataagc ttttaggata catctctttt gattgcgtat
    13261 tatgttattt agcgcgtgac gcaaataagg agattgaaaa aattgctgtt ttgtatgaag
    13321 aaaccttgcc tggtattcca aaaatcggtt ttgggacatt cagtgagaac ttctgtggag
    13381 caaatgtaaa tcaaacggaa acctatctag caataactag aaaatagcga ataacgttta
    13441 aaggtgattg acacaataaa gttaaagagg caaaaaaccc tttaacttta ttctcaaacg
    13501 aatcacacta tagaacatcc acactttaat taaatctttc actctcctca agatgcgtag
    13561 taatagaaaa taataagttt tctactctct cccatttata tttttaggga atccttaata
    13621 ttgctgaata ctgaattcat aattaaaagt cctaattttt ataaaaatta ggattaaact
    13681 aaaatatcta tttgatgaat caatgcatcc aactccaaaa ctcttggatt atttctatca
    13741 actaactttg cattttttaa cattgctgta gctctatgaa tattcttcaa tgcaatataa
    13801 atttcagtaa gtagaattaa attggaaaca tttctaggct ctctcaattt caatctctct
    13861 gcataatcag cagcagattc taatttacca acttgtttat aacaattagc cgcaataaat
    13921 aaaaaatttg tttcttcagg aaataaatct agatattcac taaaatattc tcctgcttta
    13981 gcaaagtttc ctttgctata tgcgatattc gctctgatct ttaaaacagt tctattgatt
    14041 tgtctcttca ataataattt ttcgattatt ttctcagctt cgcttatatt tcctgactca
    14101 tacaattctt tcaaatgaag actatcattt gtttcttctt ctaggtaatt atctgtattt
    14161 ttatattcga ttctaagtag gctataatca tcgattaatt cccctttcgt ttgagttaaa
    14221 taaaatattt tctctagatc tgcattggct tgttctacag tttttaaaaa taaattttca
    14281 tcctcgttaa ttaatctaag cccaaaatca tccatgccaa ttttaatatc atctcttcca
    14341 tcagaaccca taatcacaac atcatcatgc tctaacttaa atgtattcac ttgaaaatct
    14401 ccagataact cttccgtcat aattccaatt tttcttaaat tcatttcttt ttctatgaat
    14461 gatgctttat tatctctaaa taatacacta aaaggatgtt ctgcattaat ataataaata
    14521 aatcctctat cattatctat taaccccatt acaacagaaa tcatcattga tccatcaaag
    14581 gattgaaaca cactctgtag ttcttgaaat gccattttca accaacgttc tggataaaca
    14641 gtatcctgac ttgtaaatct tgctctagat actatcgact taaaaacaac tcctaataca
    14701 agagcaccgc cagctccttg aattgatttc cccatagcat ccccatttac aaaggctgta
    14761 aactttcttc cctttaagaa aatattatcc gaaatacaaa tatctccacc gatctcatgc
    14821 attctatttt taaattcaaa tttcttcttt tgatggatta aaattttaag atcaattttt
    14881 tcatcatttg cagcgttaga tgctagagga ttaataataa gagtagttaa gaaatagtca
    14941 ccatcctgtt gttctttgag taattttact tgtgaaagtg tttcctggag ctctttcgtt
    15001 ctttgaataa ctttatcttc taacgtctta tttagatttt caacagtttc atgtaaattt
    15061 aaaaagcgat tagccaaaat ccctacaatt ccaactataa aggagaaaaa accaaaacgt
    15121 gttaaaatta aacccgtctt aaaaaatatt gaatctaaaa catcaaaaat agcagaaaaa
    15181 atcattattg taagaccaag cattaagcgc ttagccgaat cggttttttt ctgaatcgca
    15241 actttaaaaa cataaattaa aaatatagcg gaaactaatg cgctaatttg ccatgctcgt
    15301 aatacataga taataaaatg ctgtgggaca aatggagtca caataaacaa aagagcacta
    15361 tatagaatat atcccttaaa atatcttcca atctttccaa aaaaaagtaa ttctaaaaag
    15421 attcctagag ataaaggaat aaaataaaca gacatcagtt cgattctata accaatcgtt
    15481 gaatcaagac catattcaaa aaataatcca tatcttccaa ttaagtaaat aaaggcactc
    15541 agagataaaa tggaaaaagc taaattatag gtttcatgtc ttcttttaat gtataaaaat
    15601 aaatggtatg caccaaaaaa taaatagagg aaaattaata ttatccccgt tctatctcta
    15661 ctagaaatta gaatcttatc atactcatct aaataataac cgttagcacg gaagagtccg
    15721 gttaaattaa acttaggatt cccttgaata tgcagaatta aaatattttt atctttctta
    15781 aaaaaagaga aattcaatgg atatttgata tgattcccat aacgactcag gacaacttta
    15841 ccattctcca ctttaccttc ttgtccaatc atttttccat taagataaat cgtatataca
    15901 tcccctatcg catctaggta aattccagtt gtgatttttt cattaaagta ttcatctgaa
    15961 actttaaatt ctgtccgcaa agtaaaatct atactttctg tttgaggata aatcttttgc
    16021 acactgactg gaaatttctc tatttctttt gaaccctctg gaactgtgtc cgattgaata
    16081 tagtcttctt taaaattatc aataatatac cagggtctct gcttacattg attttccgag
    16141 ttgcagtctt ttaataaatc gacttgctct actgaaaaaa tgggaaaaca aactagaaga
    16201 aaatgtaata ggaaaaaata tcgaaattta gatttcataa acattttgat ttaaaatcaa
    16261 attcattcgt tggtcaagaa gaaacaattt ttttccaaaa aacaattaga aaaataaatc
    16321 aagtattcaa gattcttaat tttatttaca gatatctatt gcaaatcaat ggtttaataa
    16381 aatgaattgc ggcttagaat aattcagttg tatgattcat gaaaaatgag atttatacta
    16441 tcagaaaagt ttttcctagc taaaccgata gttgatatag aaaaccatct taggagtttt
    16501 tgataatctt ttattagaag agtttatttt tctttgctac atgaaaaata tgaccttctt
    16561 ttttctgttc ttttaataat ttactatgag atagaatctc aaaaataaca tcaacaggta
    16621 tactctttaa actttcaaat tcctctttat attgggtaag aggaatttta ttaaataaaa
    16681 taaaagtttg gtattgtcca atctggtgct ccattaagaa ctttaacgtt tctaaatctc
    16741 ctacggcttc agcggtaatt acagcctctc tattatcagc tagtttttta caatagtcta
    16801 taaatgctag attgtctaaa aatttaacct tgtcttcggt cgctttaaac ttgaaactaa
    16861 ttggatctag tttaaattct tgaatcataa ttccaagatc taaaacgatc tgatggcttg
    16921 aactttttac tccaaaatcg tcagctgcaa aactatagcc ataatcccaa aaacatctgc
    16981 aaacatcctt taataaaaac tcatcttctt cataatgctt ctcgataagt tcaaagcgaa
    17041 cattctttgg aagtaaattt ctagatcgaa tcaaactatt aaaacgattt accttttcat
    17101 ggtttgaaaa tgtatcaatc aatgattgtg gagtgatatt aaatttaagt aatccagggg
    17161 cattttcact ggctagtatc aacttttcaa ggattagcag ttcaacacgt ctaatatctt
    17221 ggtcagtcgg aatatcagat attaaatctt tatatccttt gtatgctccc ccgccaacaa
    17281 agacctctcc tcctttgaca gaaaaagttt tttcctttga atcatagcaa acagtcggtt
    17341 gaatcatcgc ctcatgagca tttcctgaaa tataactgtt tgctttatta aaataggtcc
    17401 atccccaacg gactaagtta tcatttagat ttttttcaga tgttttatac aattcatcaa
    17461 agatttcatg catactagaa atataattgc attgagttct tgaaattcca tagctaaatt
    17521 caattttctt tttgcgaaga ttctcttgat gaatacgacc aaaatgggaa tctaaattcg
    17581 gaaaagcatc atccgcaata tccccacctg atgcaatacc aagaagtaaa cttcgcttat
    17641 tctctacaaa ataaaatcca taatctacaa ttttaatttc aggcaagttt tggatttcat
    17701 gctttataaa atgaataaaa tcagctaagg atattccttt aatatgctcc atccgaacca
    17761 tgaagattgg acgaccacga tgagtatcaa taaacttttt cttaaagctt tctaaatctt
    17821 gtaaatcctg taagacttta tcatatgtat cgtcttctga ctctagttca actttcttca
    17881 tttaaaaatc ctaagctaaa atatctttga tcatttgata atacagatcg caactcaatc
    17941 aaatcattaa atataacatt gataaaacaa agaatgatca taaacacact tactgaatgc
    18001 attcgttaaa tcatctttga attcatatta ctattaggag cgaggtatac caattacttg
    18061 cgaaattttt tacagaaaca aatcaatgtc aagtggattt taaaaattct aaattctgcg
    18121 gcaaactcaa ataagcccgt ctttctattc tatgattttt gtttgaagtt ttactgattg
    18181 agggaggaac ttaaaaaaga taacccttgc tcgacaaggt taagggagag agcaagggtt
    18241 agtaaaatat gtatgagatg ttaattattc ttctagttta gctaattttt gatattctgc
    18301 tggagagtag ttgtttgcta aattttcgta gctagctgct aatttatcaa gagactcagc
    18361 gagatttaaa agttttttaa ctcttaattc ttgagttaat gctttgccac cataattcac
    18421 tgttgccatc tctctgtaag aatcagctag ttttttcttt tcagccgcta cagaatttaa
    18481 gtaatgagca actgcagttt tttgttcttt tgtcactgct ccttcgatga gtgctttttc
    18541 aagtaatgaa ctctctaaat ctgaactgat tgagaaaatt gggcttacca ttagggtaag
    18601 ggaagcgatt gttaaagcga ttgttaaatt tgatttcatg gtgcatatcc tcttgtttat
    18661 cttgtttctt ttcagctttt tgctgattta ctttatatat agcacaaagc gtgccaactc
    18721 aatacaactt aattttaatc ggagcgcaaa acgaatcaat tttcataagt tatgtctaat
    18781 atgctttaaa tagccttata agcttaatat aatgaggctt tttttttttg ccgaagattt
    18841 cattttcatt ttcttctctt ttcaatttca ataaactaga caaattcatt tctggataga
    18901 gaaaagatta attttaatcg attaaatttt gaatatttgg attattttta atcttatatt
    18961 gcaaagatcc ccgacttatc cctaaaagtt tagctgcttg ttcttgggtc ttaccaattc
    19021 tcatagcttc ttggatcaac tttgtttcta cttttaataa agctttctct aaattcaaat
    19081 ctgttgcatc aatctcgact tcttcgggac tacttaaggc tttggattga ctagtctcct
    19141 cttttaatcg atcagaacca cgaattgcat tatcaggcag aaaatctggt gtaatattat
    19201 cgtttggaca aagaatggct gcatattgaa tggcattttc gagttctctc acattgcctg
    19261 gccatgtgtg ctgagttaat aagtctagag tagacaaatc aaaatattta ttcttcccat
    19321 aacgtttatt aaattctagt aaaaagtgat gcacaagcaa gggtatatca cctcgtcttt
    19381 cgcgtaacgg tggtaggcgc agaggaatta cacccaatct aaaatataga tccaatctaa
    19441 aatttccctt ttccacttct tgtgcaagat ccttatgtgt agcacttatg actcgaacat
    19501 tcactttgat tttcttgaaa tctccaaccc tttcgatttc tccctcttgc aaaacacgga
    19561 gcaattttac ctgtaaagaa ggtggcatct cagccacttc atctagaaaa atcgttcctc
    19621 cgtttgcagc ttcaaatcta ccaattacat ttcgatcagc tccagtaaaa gctcccttta
    19681 catgcccaaa taattcggat tcgagtaaat tttcattcaa ggctccacaa tttaaactta
    19741 caaatggatt tgacttgcga gaagatagat aatgtaaact ctttgcaact aattcttttc
    19801 cggttccaga ttctccatta attaaaacag atgcatctga aggagcgaca gttctaatcc
    19861 tagtctttaa aaaaataatt tgtgcgtcat acccgagtat tgagttcaaa aaatcttgct
    19921 cttgtttgct tctctccata gcttctcggt aaaaagtatt ttgaacaagt gccttagaaa
    19981 gctcgtctgc aattttttct gcccaagcta tttgtttttt ggaaaaatga tctaaataat
    20041 gagactctaa atttaatata cctattgttt catcaccaga tatcataggc actgctagtt
    20101 cggaagcgac agaatcaatt aatttcacat atcgcttctc tttagtaaca tccggagcat
    20161 atatcgtttt cttgcttgcg gctgacgctc cagtaattcc aacagcaaat ggaaattttg
    20221 cggcaatttt tttttcttta tcgatccctt tagcacacgc gatagttaaa accttctctt
    20281 gtggatcagc aagagatata cttccagtgc ttgcttcaaa gatacttaga atttcttcca
    20341 gaatatgatc tagaatatca aaaatgtttt ttcctaagcg aatttcgatt tgtaaattat
    20401 taatcaaatc ataaaatttc ttctcttccg gcgcgatttt gtttttggtc atattttatc
    20461 atctcattta catattttca tttccctttt ttatttttat aaagaaatga attgagcttt
    20521 caagctcatc ctcttgttta tttagaattg atttatatac caaatgacaa aaaaaattta
    20581 ctataaggat ttcgtttgag tcgtatttgg aataaaataa aatttacaat tcgttgctta
    20641 caaactaatt ctaaatgagg ataagtaaac gaaaaattta taaagaatca aaagaatatg
    20701 atgtaaaatt caatacaatc gaatgtaata aggaagaagc ataagagtca ttcaaaactc
    20761 attcccatta cttttcgtaa acgaaatggt agaaataaaa atttagtaaa tgctttcctg
    20821 cactattaaa aaacatatag ttaccacaaa tatgttaaaa cctgctaaat ccgataaaga
    20881 cgatggatgc ctatgggttg tagatataat aaggttttat tattctacat aatttgttga
    20941 tttaatcccg tttcagtttt aaatattcat cctttaaaaa tatttaatcg aactgattta
    21001 accaaaataa gcaatccatc ctattctgtg gtagtctgtt aaaatatgtt actatgatgt
    21061 gtaaaaaaag tggatacagt tggcataatg tagtcaaaat agaattcttg agaaacttga
    21121 taatcctctt cttttaagaa ttgtttctac tttaagaact ctcgctcttc ttctgtttag
    21181 ctattttctt taagcgagag aatagaatgt aaaaaaagct tatttatcat ccatactcag
    21241 aaatccgttt cgatgctata ccgttatgcg ctttatctgc acttttctaa ttttatagat
    21301 gtgggttgta ttacttgaat catgatacta gttccgttca gaattatact tgtatttgtt
    21361 atcacaaaga ctttattggg atagtaagaa ataacattga ggtattagaa atatcaggaa
    21421 agcattgaat gccttccttt atttaccgtt tgtttgcaaa ttatgcagca aatccaatcg
    21481 tcctcttttc tcctttaaaa tcaaatttct cttcttttaa attgtaaatt tcagcgagag
    21541 tcattggctt tgtagcttta taatttaacg atagactctc taataaatga ttagattttt
    21601 ctacactcag ggcagcaaat tcatataaag ctttcaatct tcctttgcgt aagagtgctt
    21661 tgtcaattcg agacaaatgg gtgttaaacg tgcaaataaa ttgaatccct agtccttctc
    21721 ccaaaagacc gtcagttaaa tttaaaatca tagaaatagc tgaattatct tcagcatcac
    21781 gagagacaat cagattttct gcatcttcaa taacaaaaat tgaatttgga ttatcgatca
    21841 agaattttgt gaaatcaggt gaatctaaac tactagcaaa tcttggacta ataaagatga
    21901 ctgttttatt tacaagatga atgagtttac gaatataggt cgacttaccg gttccaggtt
    21961 gaccatgaaa taaaaataat ccactttcat ttttttgttt tatctttttt agaatatcct
    22021 tatgcaataa ttccaaatcg ctattgtaat gagtttctat tttaattttg ggtttactga
    22081 tttgaatcga agctttttcc aatgcattaa attttccagg tattataagg tgaatatgat
    22141 ttaatttagg ttttcgtttc ttgaattgaa tcagaatctt tacaaattca ttcactaacg
    22201 cttccgcttc cctcccgtaa ctaattctga tccatgcagt attctcgctt aaggcttcta
    22261 tttctattaa tagttttgtc ttgaatagca aaaaaagaca atcaagttca aattgatttt
    22321 tatcccgatc aaagtatttt acttcccgct tatcctcgat ttgattcgaa tccattttat
    22381 taaaaaattc taagcattta gaaagattta aacctttcaa aaataagact ctaggagtta
    22441 ctccatagta aacgcaaaat aaacgcacag aagaaattct atctgtattt tcgtaaaata
    22501 tattcgggac tcctgatttt gtttttttaa aatgattgcc tctatccggc ttcatatttt
    22561 ccatttttct ttctcctaat cttttcttga aaaaattttc atatagtttc attatataga
    22621 agcaatatat cgaatattat ctattatctc ttgtgtaacc gttattcccc tagactcaat
    22681 cgaatgaaat agaaaaatca taaacggtaa atcatcctta ttctcttgac tgtaatccaa
    22741 gaaactattg tataaagctt tacaccgagc tgtatcttta ttggctatag caacttctat
    22801 tggagctaaa gtcgttgatt caattgtaga tttcatttgg tttcctcttt gttattttta
    22861 ttcattttaa aggagaaacc gattcagatt cggaaaatgt ttttccgatt aacaaagtct
    22921 atttaaaaaa accaatttga cgtatgaaaa gtggtaaatc aagatgaatt gaactaattc
    22981 tttatggcta aagaaaccac aacaaaacaa gaaagaatac tcattgcact tgaatacgcc
    23041 tgccttttgt tttatgagtg gagtaatggt aaaaagaaaa agttagaaga cttctacgga
    23101 tttttacaat catttgattc attagttcat tcagaaaata agaagatgcc agatccacag
    23161 aatttagagc gaaattacag aaattatctt tccgatctag aggcgaattt tgatttaaca
    23221 gtttccttga agaaaaaaac agtccaattt aattcggatg attctttaga agagattgca
    23281 tttaacgttc tctcttttta tataagacat gcatttctaa aacaaactga agttcataac
    23341 gattctagta ttaaaaaatt tatacgttat agattgatac tttttccatc taaaaaagaa
    23401 gaaagtttta taatggaaga gctctatctt tttgtgttta ttcgatatgc aattcaatat
    23461 aatttaaaac tggaaggttc ttacaagaag ttgatgagta attcaatctc tactcgtcgc
    23521 atttaccctt gttatttgtc aacggactcg caatatttaa ctttagtcgc aaatgatatt
    23581 tcagataata cgaaaaaaca attcattcta gccaatctcc atttaaaacc taaaatttct
    23641 ttgctaaatg aatttataaa aagccaaaga gtgaaagatc attttaatta taaaaaattc
    23701 aaacaatcaa aagaaggtcg atttcaaaga gaagaaatct tttataaaat tcgcataaca
    23761 tcgtattcac tcgaacactt attactcaat tatgattttt ccataaaaat tttagaggat
    23821 agaggaagct ggaaaataat cgagctcact acctcagatg caatcgaatt acagaaggct
    23881 ttatttgcct atgacgtcta tgcaatcctt ttagagccaa aagaggaaat ccaaaatttc
    23941 agagagaagt tgcaaagaat tttgaaagaa aattgtattt gaatttaaag gctttctaat
    24001 taatttgcat ccgtatggtc aattcaaatc aatctaaacc aatcattgtg gcaattcttg
    24061 ccaattcaat tattgcaatt gcaaaggcaa ttggatatct attcagtggc tcatccgctc
    24121 tcctatcaga aacaattcac tccattgctg atgtgatgaa ccaatcctta ctcttaatcg
    24181 gaatcaaaag aggagagcag gaagctacaa atatttacca ttatggtcta tctcaggagc
    24241 gatttttttg gaacttagta tctgcaatgg gaattttcgt attaggttgt ggtgttaccg
    24301 tttatcatgg agtgcatgat ttagttgctg gagcgcctca taaacaaggt gggatagaac
    24361 agattatcat ccaaatcatt ttattcattt ctatgttagt ggaaggttat tccttttatg
    24421 ttgtgattgg agaaattaaa aaacaagcaa gtagtagaac aaagagtttt tttgaatatt
    24481 taagagaatc aaccgatcca tctatcagcg ctgttttctg ggaggacttt gctgctattc
    24541 ttggactact attggctctt gttggtattg cgttaacaga gtttactggt aacaatacgt
    24601 ttgatgcatt cgcaagtatt ttaattggtc ttttaatggg atggatcgca attcatctag
    24661 cgatagagaa taaaagattt ttactagaca gagcaattcc tgaatatgaa ttaaaacaac
    24721 tcctagaagt tctagataaa aatccaaata taaaatccta taaagatgta agaaccgtcg
    24781 tgttaggtcc gaaccgatta aaatttaaag ctgagttaga attggataca gagaaaattt
    24841 ttgaggaatc cgtaaaaaag gccgctaaaa aggtgcgcac cacattaaaa cacgtaaaat
    24901 ctgatgaaga actagaggct gtttttttta atttaggaaa tacactgtat gcaaataagc
    24961 ttgccgagtt agaaaaaata aaaaaacagt taaaagataa acttccaaat ttacaacatg
    25021 taaatctggt gattagctaa ggagcaaatt ttgaaaaaag cacttattac aggaataact
    25081 ggtcaagatg gatcatatct tacagaactc ttattagaga aaaattatga agtgcacggt
    25141 attgtcagaa gatcgagtct ttttaatcga aatagaattg agcatctaaa agataatcca
    25201 aatttacatc ttcattatgg ggatatgaca gattcaagta acctgaatcg tattctagag
    25261 aaagttcagc cggatgaaat ttacaatttg gctgcacaat cccatgtagg agtttctttt
    25321 gaagtgcctg aatatacagc cgaagttgac gctgtcggaa cacttcgatt tttggatgcg
    25381 ataaacgaaa caggcgttaa aactcgcttc taccaagcat cgacatcaga attgtatgga
    25441 cttgtgcaag aagttcccca aacagaaaaa accccattct atccaagatc accttatgca
    25501 gtagccaagc tctatgccta ttggattgtt gtaaattata gagaggcttt tggcattcat
    25561 gcttcgaatg gaattttatt taatcatgaa tcacctagaa gaggagaaac attcgttact
    25621 cgaaaaataa cgttaggtgt agcaggaatc aaagctaaaa agatgaagac actcactctt
    25681 ggaaatttag atgctagaag agactggggt tacgctcctg actatgttag aatgatgtgg
    25741 atgatgctac aacaagacca accagatgat tatgtagttg ccacaaatga aatgcactcg
    25801 gttcgagaat ttgtcgagct ttccttttcc attgcaggga ttaaagttga gtggaaagga
    25861 agtgcagaaa aagaaatagg aatcaacgct gctactggag aaacaatagt cgcaatagat
    25921 cctagatatt atcgtcctac tgaagtggaa caattgttag gaaatccagc aaaggcaaag
    25981 gcaaagcttg gttgggaacc taaagtcaaa tttgaagaac tagttaaaat tatgacgatt
    26041 gcagattgtg aggcatacgg aataaaactt taattaaatc agtaataaaa ataaatttga
    26101 aaagaaacag agaagtaaat ttctctgttt tttttatgag taataaacac ttatttcctt
    26161 ttcaaaacag tcttctctat ctctccattt aaatcattat ttataaaagg gaaaagattc
    26221 gtcttttcaa ctcatctctt cttaaattaa tgatttgtaa aaaacaaata caaaaaatag
    26281 tttaacatca agttataata ttatgatcga agtagaacat ttaagcaaac attttaaaat
    26341 caaaaaaaga aaaccaggaa tacttggctc cattgtttcc ttagttcata cagatgtgga
    26401 atatctaaaa gcagtagatg atatttcctt tacgattcaa ccaggcgagt tagtcggata
    26461 cattgggcag aatggagcag gtaaatctac tacgattaaa atgcttactg ggatactcac
    26521 ccccacctca ggaaaaatta aagtccatgg cttagatcca tccaaagaca gaagatttaa
    26581 tgcaagacaa ataggtgttg tcttcggaca aagaagtcaa ctttggatgg atttacccgt
    26641 tgaagaatct ttcgatttac tccgctctat ttataaaata gatgaaaaaa tttataagca
    26701 aaaattagat ttcttctttg agttgcttgg tctagatgaa ttttttaaac aacaagcacg
    26761 taagctctct cttggacaaa gaatgaaagc cgaccttgca gccagtctca ttcactctcc
    26821 atctgtatta tttttagatg agccgactat cggtcttgat ttacttgtaa aagaaaaagt
    26881 aagagagttt attcgaaaga ttaacacaga agacaaagtc acaattatct taactacaca
    26941 tgatatacaa gatatcgaat ttctagcgca aagaattatc atcattgaaa aaggcaaaaa
    27001 agaatatgat ggagacattg ctagttttaa taaattgctt ggaaatgatt caattgttac
    27061 aattcatttt gtaaacccag tcaagaaatt agaactacca agtcctttta ctataaagaa
    27121 gaaaatctca gatcaacagt attctcttga tgttccggac aacgttcccc tgtctggatt
    27181 actcgaaact ttacaaaagc agggattaca aatccaggaa atcaatcgac acaaaccaga
    27241 tcttggttat gcagtaaaaa aaatgtatgc aggaaagggg gaattatgac ttatttgaaa
    27301 ttcatttcaa aagcatttca gagatcaatt gcctacaaac ttgaatacta cactggactc
    27361 atgaatgcgt ttctatatat tttcattttc acatctgtct ggcaaaccgt tgcaaaggaa
    27421 agtccgagtt ccctaggaac atggacagga gaaacgttag ttagttatgc aatcctttct
    27481 acactaataa aagtttcctt tggtagaaat gaatcactac ttacaaataa aattaaaacg
    27541 ggtgatatta tatatgatat tttaaaacct tacaattttg ttatgatgta tttcgcagat
    27601 agttttggag taagtctgtt tcaattattt gcgagagcta ttcctcttct tattttttca
    27661 atattattct ttggtattgt tccaagtgta acagcgatta gcctccttca atttatacca
    27721 gtttatattc tttctttcat cctatttatt ctatttggat ttatgatttc ctctcttgca
    27781 tttttcttca cagaagtttt tagcttcatg attttataca gtgctcttgt gactctcatg
    27841 tctggatcag taatcccact gaatttattt cctgactatt tagtagagat aatttcttgg
    27901 agcccctttc cttatctata ttactatcca acaactgtat tgattggaac tcctattcaa
    27961 atgtcctatt ctgaattaat acttaggtat ttactagaat tatctatcgt tggtagctta
    28021 actttcacag cttatttttc aggaaagagg aagctcgaat ttgcaggagg ttaatatgaa
    28081 tttaccccat aaattaatca aaaacgaaaa tacttttata gagacagttt atatttacat
    28141 tcgcatagca acagcttcta ttaaatccag aatggaatat agagcgagct ttcttgtttt
    28201 tctatttaca cttcttactt tttatgcagc tcaaatttca accataggta ttgtaattac
    28261 aagattcaag aaaattggcg gttggtcgat gggcgaaatg gcattcttat atagtctatt
    28321 gattttatca caaggaatcg ttacatttat tttttcagga atcctagatt ttagcacata
    28381 tgtaagagat ggaacttttg atcgtttttt agtcagacct ctttctccat tatcgcaagt
    28441 tattatggga ggctttgaga ttactggatt tgcacatatc atactaggat tcctaacgtt
    28501 ttttatggca aatacactaa tcactgttta ttggaatttt acgaatgctt ttattttttt
    28561 agcagtagtc attggtggcg ccttaatact tggttcgatt cgaatcatta ttgcggctat
    28621 agctttttat gcagtaaata caaattcatt ggtccatcta tttgtttttt cttctcgaga
    28681 atttttattg tatccactta atatttacag tagtggactc aaatttcttt taacatttat
    28741 tgttcctctt gcatttatta atttctatcc tgcacattat tttttaaata agacaggtga
    28801 aaatttattt catccctatt ttgtatttgg aactttacct gtggggataa tcatgattat
    28861 cttctcactc tggttttgga gaaaaggaca ggatgcttat gaatcagcag gcggctgatt
    28921 ttaaagaata attttcttgc ctcgtaagta tctttaaata gtttgaattt atgtatcagt
    28981 ttccccataa gaatatctta gatacggatc aattttcaaa agaagattta gattttatca
    29041 ttcaaaaaac tagaattatg aaaaaccttc tgcattctgg aaagtcattc ggaatgcttc
    29101 atggaaaatt acttgcttcc ctcttctttg aagcctctac tcgaactcgc ttatcctttg
    29161 aatctgcaat ggaacgacta ggaggaagag taatctcaac cgtaggattt caattttcct
    29221 ccatttcaaa aggtgagacg ctttatgaca caatgaaaat ggtcgaagct tatgcagata
    29281 ttgcagtaat ccgtcatccg gtagaaggct cctctcggat agcagcaggt gctgtgaaaa
    29341 tgcctgtaat caatgcagga gatggggcag gacaacatcc cacacaagca ttgcttgata
    29401 tgtataccat caagtcggaa aaaggaaagt tagacgatat aacagtaggg tttattggtg
    29461 atttaaaata cggaagaaca attcattctc taataaactt actcaagcat tataaagttc
    29521 atttatattt aatttctcct gaagagctag cattgccaga taactacaaa aaagatgtag
    29581 caggtcactc tctgactttc gaagaaacta gagacattaa aaaaatctgg aattgcgacg
    29641 taatttatgt tactcgaatt caagaagaga gatttccaga tcataaagaa tacgatcgac
    29701 tcaaagaaac ttataaggtt aataaagaat taatactggc atctaaaaaa aatacaactg
    29761 tattacatcc gctaccaaga gtaaatgaat tgtctacaga cgtagatgat ttaccaaatg
    29821 cagcttattt tagacaagca gaatatggag taatcgtgcg tatgacgctt ttatgtctat
    29881 gtttgggtat cgatattaat gaggcttaga aatgtttaat cctaaaaaaa tttggacact
    29941 agaagaagct cgtgaagttt ttccacaaat taagaaaatt acagaaggct ttcatgcgaa
    30001 ggtagaaagc cttgttcacc tccttactga aaatatctat cctgaaaacg aaatggaaag
    30061 tatggaggat gaaatcacgg atttactaaa agcatggaac tttactttgc aagaatttga
    30121 agtagacgtg aaaggtatct ggctagtaga ttttgacaat ggaaatggct actattgttg
    30181 gaaagttgga gaagaggatt tattgtatga acatacctac gaagatggtt tcgcaggaag
    30241 aagattaata gaaaggaaca aacaaaatgg cgattattaa cgaaaactat ttaaaattga
    30301 aagctggata tttatttcca gaaattggaa aaagagtaaa aaaatattca gaagaaaatc
    30361 cagataaaaa aattattcga ttaggaattg gagatgttac acttccgctt ccacctagta
    30421 tagttaaggc tatgaaagac gcgtcagacg aaatgggcac ttcatctggg ttcaagggat
    30481 atggtccaga acaaggttac ggatttttaa ttgatgcaat cattcaaaat gattttgcat
    30541 caagaggaat caaactaagt caggatgaag tgtttgtatc agacggctcc aaatgtgata
    30601 ccggaaatat ccaagaaatc ttttctatag atagtaaaat agctgtaaca gatcctgttt
    30661 atcctgttta tgtagatacg aatgtaatgg caggaagaac aggtgcagct ggctcagatg
    30721 gtcgttatgc gaatttgatt tatcttcctt gcacaaaaga taataatttc gaaccagagc
    30781 ttccaaaaga aagaccagat ttaatatatc tttgctatcc aaataatcca actggaacaa
    30841 ccattacaaa agaaaagtta aaaacttggg ttgattatgc tcataaaaat aaaagcatta
    30901 tactctatga tgctgcttat gaagcattta tcacagaagg tcatttacca aagtccattt
    30961 ttgaaatcga tggtgcaaaa gaagttgcta tggagtttag atcgttctct aagactgctg
    31021 gctttactgg aacacgttgt gcttatatag tgattccaaa agagttgatg ggttataccg
    31081 gatccggaga aaaagtaagt attaattctc tttggagtag acgtcatacg acaaaattca
    31141 atggagtatc ttatccaatt cagaaagctg ctgcagcttg ttactcagaa caaggaaaaa
    31201 aagaagtaaa agcaaatata gaatactata tgtctaacgc taatttaatt cgcacttcct
    31261 tacaaagttt tggctacgag gtgttcggtg gaattaatgc tccttatata tggctcaaga
    31321 ctccgagaaa tttaaattca tgggatttct ttgatgaatt gctttctaag gttcaagttg
    31381 taggaactcc tggttctgga tttggaccat ctggagaagg tttctttcga ctcagcgcat
    31441 ttggaacaag agaaaatgtc caagaggcaa tgaatcgaat taagggattg taaaatagaa
    31501 atatttaact gaaagagttt taggacaaag caaaaattct atcctaaaaa ttctttcagt
    31561 tgaatttgat gtccttctat ttctattgaa tctttagaac gatcgatttt cttttcaaga
    31621 tattttcctg aattcggttc tttatatacc tcaacttcta tttcttttaa attgattagc
    31681 cagtattcca tcacaagtgc atctgcataa ataaattgtt tttgaaaatc taaatcgtag
    31741 gtcgttaggc taacttctat gactaatcta gctgttgctg gatgctctaa taatgtggac
    31801 aggatttaaa tctagtattg ctttttattt ttgtcacatg aaaaagttaa acttctttgt
    31861 atagcagaac tgtatcttcg gcagctttct tccaagtaaa tcgtttcgca ttttgatatc
    31921 cttgttctct cttggatttt aaaagagagg gatttaacat aagaagtcgg aattcttctt
    31981 ggaatttttt ttcatttcta ggatcaaaga aaaaagcaga atttccaaga atttctggca
    32041 taacggaagc gttagatgaa aagactggac aaccaacacc ctgtgcctct aaaagaggaa
    32101 acccaaatcc ttcatagaga gatggaaaga taagcatttt agctccctga tagagtaaag
    32161 gcaattcttc ataagataat ttacgaaatg gaagtataaa tttttcaact ggttgaatca
    32221 tttcttgcag atggtcagga atttttccac ttgttcccgc tagaataaga ggtaaatcaa
    32281 gattaccccc aagccataag gggatgaggg attgaattac aaattgtaga ttcttatgac
    32341 cttttccaat tccaacagtt aaaagataat ctttttctaa attatatttt cttctaaact
    32401 ctttgacttc tttctcttta tgaggttgaa ataattcttg gtttaaaccg ttgtaaatgt
    32461 tatgaatctg ttcaggagaa aaatgaaact cttgaattaa atcttttttt gtgcattcag
    32521 acacactgat tatttttcta ctaaatcttc ggatcaacca aaaaataaga cgaatataaa
    32581 tttgctttgc tttacttgga aaaaattctt tcatcttata gggaatgata tcatgtatcg
    32641 taacaacgca tttttttaaa tagagaattg gtatattaaa atgaggaata tctaataaat
    32701 ccatttcttt cattttagga tgacctaaaa attcttttat actataaacc ttcgtattgt
    32761 attcaataat tggaaatttt tgatctaatc catatttact aagaatctct ctttctccaa
    32821 ataaataaag ttcaaatcca gaaaggtttt gttttgctaa atattctact aaattgagaa
    32881 tacgaactcc tataccggag tgctcgatca ttctaacatc gattccaatt tttttcatga
    32941 gagtctgtta tatccattta atgccgcaac acggtaagct tctgccattg tgggataatt
    33001 gaaagtagta ttgataaaat agagtagatt atttgcatct cctttttgtg tcataattgc
    33061 ttgtccaata tgaacaatct cggatgcttc acttccaaaa caatgaattc ctaatatttc
    33121 taatgtttct ctatgaaata aaagttttag cattccaaca gttctacctg taatttgtgc
    33181 acgagctaaa cttttaaaca aggcatgacc aacttcatag ggaactttcg catctgtaag
    33241 ttccttttct gtttttccga ttgaactaat ttctgggctc gtatagattc ctgttggaat
    33301 attatgcact aatttaaaat cgcattttcc ttcaatgata tgagtcgcgg caaatcgacc
    33361 ttggtcataa gcggcactcg caagacttgg atatccaatt atatcgccta ctgcatatat
    33421 atgaggtaag acagtttgat aattttcatt tacatcaatt tgctcccttg aattaacagg
    33481 gattccaatt tgatctaatc ctaaatcttt ggaatttcct gttcgaccat ttgcgaataa
    33541 aaatacatct gatttaattt gtttacctga tttcatatga agtataacac cattagccgc
    33601 aaattccacc ttgctatact cttcattgtg tctgattaaa atgccttgct cccgtaaatg
    33661 ataggcaagc gcatcaataa tttcatcatc taaaaaagct aagagtttat ctcgagtatt
    33721 tactaaattt actttagatc caatacttct gaaaatagaa gcatattcac aaccaatgac
    33781 tccagctcca tagattgtaa tcgaataagg agtatgattc aattttaaaa tagtatcact
    33841 atcaaataca tttggattcg taaaatcaat atccttagga tgataaggtc ttgaacctgt
    33901 agcaattaca aagtaatcag cctctaatct agcaataata tgttggttgt ttctaacttc
    33961 gatagtatga gcatctgcat aagaagcatg accataataa atatcgatat catttctctc
    34021 gtaaaaagac tgacgcattt tgacttgttt atttattaca gattcggcag ttttaacaag
    34081 atggggatat tcaattgtat tggtatattt ttcgaataaa ggatttgatt taaaatcaac
    34141 taactgctgt gccgcatgac gtaatgactt acttggaata gtgccccaat ggctacatcc
    34201 tccaccaacg cggtcatatt tctctataac cgcaacactc ttccctgctt tcactgcttt
    34261 catagcagcc ccttcccctc caggaccagt tcctattaca attacatcat atttcttgtt
    34321 ctgcatatca tctaattgta attattgaaa tagattgtaa atcgttttat gttgttatct
    34381 tatgatttcc acagtgtcga cattttctgt cataattttt ttgattcctg agtttgtctc
    34441 cactcgaata aaatctgttc cttgctcaat aacaactcct tttaaaaatg aattatctat
    34501 aaggcgaact aattctagtc ttccatgctt tgcacgaatt tcttcttctg tcaaattttg
    34561 aacaggagtg tctttagctt cggatggacc ggatgcggag gaactataag gcgattgatt
    34621 cttgggtggc tgtttagcta ataattttgg gctaatcttg aaatgagcaa tagcagaagc
    34681 tttcggggtg agtggagaat agagaagatc ttgccaataa gtaaaaacaa gtaatagcaa
    34741 gagcaataat gggaatagaa ttcttttcat atgagaaatc ctctttgttt tataaaaaat
    34801 actacagatg actattgcat ttttatcgta aagaatttta ttttcttcca atataatatt
    34861 tttttagaaa aattttacga aatatactag aggctaatag ccatttgacg cagtctaaaa
    34921 aggatttcaa atttaagaaa gcctgtttca tttttaaaat gagctaagta gacttgacaa
    34981 tcgctggttc gtaatttcct tttaaaatat gaatcgtaaa agatttctta ctttagctac
    35041 gagcttgatt cttataaaaa agaattggga actaactgcc gaggatacaa tgagagaaat
    35101 aaaagcaatt ccaatgagag aaattccttc ctccagagaa aaaatcccag ttttaggatt
    35161 tggaacttgg agggtttttg ataaggattc tacaaaagag aatctgataa cgggaatgga
    35221 ggtctgggaa aaatttaaag aacatggtgg taaattgatc gatagttccc ctatgtatgg
    35281 aagatccgaa gaattcattg gaaacctttt cgagaaaaag aaagaagaga atagattcct
    35341 tgcaacaaaa gtttggacaa gtgggaaaaa agaaggatca catcagatca atcaatcttt
    35401 ccaaaaaatg aaaacaagtt ttatagattt gtttcaaatt cataacttat tagattggaa
    35461 aacacaaatg gatactcttc gagaattaaa ggcagaagga aaaataaaat atataggagt
    35521 cacccattat attcctacat actttaaaca aatagaagaa attattagaa aagaaaaaat
    35581 tgattttatt caaattccct attcaatcag tttacgagat gcggagaata gtattttacc
    35641 tcttgcaaga gagaaaggca ttgcagtatt aatcaaccgt ccctttgagg gtggagaact
    35701 cttttcaaac tttattaaaa aaccgataga ccccagattg aaagaattcg atatccattc
    35761 ttgggcggag ttatttattc gatttcttgt ggcaaatcga gatttaactt gcatcttatt
    35821 tgcatccttt aattcaaaac atgtggagga gaatatgaat gcggcaaaga aaccactctt
    35881 agaaaaaaag gatttggaaa aagtgtataa aatatttcta gaaaattaat attactctcg
    35941 aaggtatttt atggtaacat ctcccatttc gatgttgtcg ttgtgtataa gtatataagg
    36001 aataccaggg gctaatttct ctccatttag aaaggttccg tttttagaga agttatcaat
    36061 taaataatag tctaattttt tcttttcaat ggtagcatgg actcgactca attccataga
    36121 attcactgga atttcttttg ttggatcaac ccctctgcca attaaatatg tttttctttt
    36181 gagtattttt agagggatca tagaagtccc gctttcaata tagagaatat ttcttttaaa
    36241 gagctttact aaaagtaatt ttattttaaa cattaaattc attttataaa tagtatcggt
    36301 aaaaaataca ttggttagaa acagagtttt cgttaacgct tagtattgaa ttctttttaa
    36361 tggataacta caaccgggtg tgatttcatt gtagcattca tcataacagc ttgctctttt
    36421 cgcatgcata atcgcaacgg aaggagcaaa gatagttgag aatacatcat agttccgatt
    36481 gcaatcactt aaacatttag cagttttact cacacaatta tgaaccggtc tcccatttaa
    36541 ctcctcttct gttttaatgc gatacatccc tcctccattg ggttgctcac ctcctgacct
    36601 agcaatttct gctctagtta aatttccatc atgagggtgt tcataggtat aaacgcgtgg
    36661 aggcttacct gcacgatccc aatctagacg aattatgcaa gaattaatga gaataacaat
    36721 gattagccac agaaaaacga atgttccttt gaacaaattt aactggttga ttagaaaaaa
    36781 cttttctaaa ataagtgcat tcattacttt agtatcagta taatcccaaa ggagataaaa
    36841 gtaaatgaaa attgacatcg gaatttcaga tgctcacaga aaaaatattg ccgaaggact
    36901 aaaatcactt ctgtctgata cgtatatgtt atatctaaaa acacatggat atcactggaa
    36961 tgtaactggt cctatgtttg acagtttaca taaaatgttt atggatcaat atactgaatt
    37021 atggaatgct atcgatccta tagcagaaag aattcgctct ctcggattct atgctccggg
    37081 aacctatgca gaaatgagca aattaacaag tgtgaaagaa gacactaagg ttccaaaagc
    37141 acaggatatg ataaaaaata ctattatcgg acatgaaacc gttatacgaa cagctcgcga
    37201 gattttcgat gtcgcagaga aaggaaaaga ccaagctacc atggatttac ttacacaacg
    37261 gttagatatc catgagaaaa cggcctggat gcttagatcc ttattggagt agaattttag
    37321 aaaaatagaa tccgctagaa ttagaaaatt cggattctat tttaaatttt attcaattca
    37381 aaatttttag agtataaatt gatttaaata tatctctgta atgcgtctgg aattgtaaaa
    37441 tcacctgatt cagtttgata gttttcaacg atagccgcta gagttctacc gatagcaagt
    37501 cctgacccat tgatcgtatg aactaattga ttttttcctt ctttggattt ataacgaatt
    37561 tttcctcttc ttgcttgaaa atccttgaag ttagagacag aagaaatttc catatagcgg
    37621 ttgagcccag gcatccaaac ctcaatgtca aatgttttag aagaagaatt tgatgtatct
    37681 ttactacaaa gaagtagcac tctataatga agacccaatt tttttagaat attttctgcg
    37741 ttagtcaaca tctttctatg ttctgcttct gagtcttctg gttttgtaaa ttttaccaac
    37801 tctacttttt ggaattggtg aactctaact aatcctcttg tatctctccc atgagaacca
    37861 gcttctcttc taaagcaaga agtatgagcc attatggaga taggaagttt gtcttctgga
    37921 ataatttcat ctctatagag attggtcaaa ggaacttcag ctgtaggaat taaactaagt
    37981 ccatcttgat ctaaacgata gtattcatcc ttaaactttg gatactgtcc cgtagttttc
    38041 atagagtcat catttacaag cattgggacc catacttctt catatccatg ttctgttccg
    38101 tgcgtattta acataaaatt cattaaagct ctttctagct tagcagcttt tccgaagtaa
    38161 gtataagcac gaccaccggc aagctttaca cctttttcaa aatcgaagat gccaagtgct
    38221 tcaccaattt caaagtgagg ctttggtgca aaagaaaatt ctggcttaac tccatgtttg
    38281 taaacctcga cattgtcctc ttctgatttt ccaaaaggaa cctcaggatc tagaaagtta
    38341 ggtagattta aattcagatc ggatagattt gtttcctctt tttctaaagc ctcttccagt
    38401 tctttgattt tatttccgac atctttcatg gagttggaaa gctcttgtat atccccgccc
    38461 ttacttttga tgatgccgat ttccttactt actttatttc tttctgcgcg taaagtttca
    38521 acttctgtct gtaatttctt cttttgttga ataataacgt ttaatctgtc tactaacgat
    38581 gtatcttcca tacatctttt ctttaacatt tctctcagtt cttctggatt ggattggatt
    38641 ctatttaaat ctaacatttt ataccgcttt ccgatgaata aattttacac tgttttcata
    38701 tattttttct tctactttat gatttgcttc tgttcttaaa gaaaacattt tctctaagat
    38761 aaatggtaaa ttggaagaat catttctctt tcctctatga ggcggaggag acaaaaaagg
    38821 agcatctgtt tcaatcagga ttgattctaa tggaaccttt ctagctgctt cctgaatatc
    38881 tgccgcattc ttaaaagcaa ctacgcctga aaaagatata taatatccaa gttctgcata
    38941 ttttaaggca tattcagtat cataggtaaa acaatgaatc acgccaaaag ctttgtcttt
    39001 gtattttttt aaaatttgat aagtatcttc cgcagcatct ctagaatgta taataacggg
    39061 taacgcatat ttaaccgaca tttccataaa atggtgaaat gtttttattt gagattcttt
    39121 ggtttctgtt ttatggtagt aatctagacc aatttcaccg atacctacaa aacttgtttc
    39181 gtctttattt tcttctataa acttggaaat gtcttcaatc tcaggtaaat caatattatc
    39241 cgcgggatga catccaatcg tataggataa ttcaatgttt gtttttgttt ccaaagcaat
    39301 ttttctagcg gcaatagagc gatctagatt gatgccaatt tggacaattt tctttacacc
    39361 tgcactactc gatttttcta gggaggttgc aatgtcctgc ccctgtgatt caataatgtc
    39421 taggtgacaa tgagtatcta tcaagctgta gttcatatac aaccataaaa tttaaaatat
    39481 ctctaaacta aagtcaaatt tcaaaagaat ggctttgcgt tatttaattt tgaaactaac
    39541 cggaatgaaa agaattgttt ccggcggtag gtggaatttc caattgtgaa ttactctgta
    39601 aaattcttct tcaaatattc tatacttaca gggatgaatt gttttgatat tagtagcact
    39661 ttgatttacg tctattgtta cactccattc acattcagat tctagtccat cctcgagtgc
    39721 ttgtggagga tagacaatct tattgcgaat ttggtttatt tcttctgcaa tacttccttc
    39781 actggaggaa ttatttttaa tttcctcaac agaacctacc cctttctctg cttgtgaaaa
    39841 attgatagaa aaaactttcg aagatccttt atcgatttct attttttctg atgtttgaaa
    39901 actctctttt tgaagaatca taaaaatggt taatagataa acaaatattg tgacataaaa
    39961 agaatttctt aagctcataa ccgatttgat cgcaaattaa ttattccctt gacagtaaaa
    40021 tttacaatca ctaaatgttt gtagactcaa atgaagatat tccgacgact tctatcttat
    40081 tcactaaaat ataaatatcg ttttatagcg ggaatatttt tcgctgtttt gacagcactt
    40141 atcaatgcat catctataac agtattaatt ccactttttg ataatttagg ttcagaaaaa
    40201 aaaacaaact ttcgattaga gctgactgct cccgaatata atattttaat caaagaaaaa
    40261 atattcggac gaaattcttt agatggttta gaaaaattaa aaagactcat cattgttata
    40321 aaattcagag taaacaatac aattaaggat atgaattcaa cagaaatcgt ttggatgatc
    40381 tgcaagttaa tcatcccact ctatctctta aagatgatcg catatctttt gtcagtaatt
    40441 tgtattgcaa cgagtggcta caatgcagtc cgtgatttaa gacaagaatt gtttgataaa
    40501 gtgcagactc ttcctcttac ttatttttat aaggagaaga ctggtttatt aatgagccgt
    40561 attattaatg atgcggaaac tgtctcagca gttatatcta gtaatctaag agatgcgatt
    40621 ataaactttt tctatgtgat aactcatcta tttgtattac tttatttgaa tacggaatta
    40681 ttactaatag catctgttac agttccaatc attatctatc ctgttactct atttacaaaa
    40741 aagatttcta aatccacttc aaaatttcaa gagagaatgg cagacctgaa tgccaacgtc
    40801 caagaaatga tttctggaat tcgagtgatt cgcagcttct ctatggaaga ttatgagtta
    40861 tctaaattca atgaaattaa tacaaagtta tcccatagaa attttaaagg tcaattctat
    40921 ctgcaagtag ctccaggctt agttgagcta acgtcctcca ttgtcgtttt aggtttcttt
    40981 gctgttgggg caaaattaat ttttaacgga agctttaccc aaggcgaatt catggcattt
    41041 ctattaacgc ttctttttct gttgagacct ttgacacaac tttcacaaat ggtagggaaa
    41101 gtaacgcagg caactatatc aggcgataga atatttgaaa ttattgacaa ggaaaatgca
    41161 gaggcacatg acaaagcagt aggtgtgcat gtaaatccaa ttcgaaattc aatagttttt
    41221 aaagatattc gctttcatta tccagaaaca aatactgatg ttttgaatgg aataaatcta
    41281 gacgttaaga tcggagatac aattgccttt gtaggagcca gtggaagtgg aaaatcaacc
    41341 ttaatggatt taattcctag attttacaat gtaacaggtg gggaaataac gtttgatggt
    41401 gtaaatataa atgattataa tctccacgat cttcgtagaa aaattggaat tgttacacaa
    41461 aatattttcc tgtttcatgg aacagttgct gaaaatattg cctatgggag accaaattca
    41521 aatctaaacg aaattattca tgccgcgaag ttagcgaatg cacatgattt tattatggaa
    41581 atggaacatg gatacagcag tgttttaggt gttcgaggac tcaatctatc tggcggtcag
    41641 agacaaagac ttgtaattgc aagagccttc cttagaaatc ccgaaatttt aattttagat
    41701 gaggcaacaa gtgcgttaga tgctcataca gaaaagcttg taggggaagc actcgataca
    41761 cttttcaaaa atagaactac ttttataata gcacatagac tctcaactat aagacgaatt
    41821 ccaaaaatag ttgtcctaga aaatggacaa attgcagaaa tcggtaatca tgaatctttg
    41881 attgcccaaa atggtcttta tgcaaagctc tatgaaagtc aatttgttcc aacacaagtc
    41941 taaacaaatc tttctatgaa actcatcaat tctaaaatta taagtccatg gacattggaa
    42001 tttagagaca aagacaaaga agatgcttat atagattcat ttattttcga ctatgctaga
    42061 aattctaaac tggtaattgg aattgcactt gtcttgggct tcattgttga tttatcaaga
    42121 gatatcaata tactttcagc cttcctctcc aatttgaatc tttttacttt tggtgttttt
    42181 tttattttta tctcgtatac aaaatacttt agaaaacttt ttaaccctct aacttttagc
    42241 tcgaatctta ttttttcata tttagctgtt actgaagtga taaaagaaaa tggtagttct
    42301 ctagaagtat tgcaaataat tatctgctat acaattttcc caagatgcta ttttaaatat
    42361 tctatcattg caaatgcatt tactatttcg tttttttctt acctaacatg ggatatacca
    42421 atcgatagtc gaaatacttt gtctgaagtt cttttctttc tgagtattac aatctatgta
    42481 gaattagcag cctacttaaa agaatatgca aatagaaatg attatctaac gaatcaaaag
    42541 ataatagacc aagaagaaaa aacaaatgaa ttacttttaa atattctgcc tgcaaagatt
    42601 gcagcggaac taaaaaagac tggctctgtt aaacctattc tctatgaaaa tgtatcaatc
    42661 ctttttactg acttcaaagg ctttacttcc attgcagaaa aaatgaaacc tgaagaactt
    42721 ttaaaagaat tggatggatt ctttttccaa tttgatgaaa ttgcaaaaag atataatctt
    42781 gaaaaattaa aaacgatagg cgatgcttac atgtgtgccg gtggaattcc aaaacaaaac
    42841 gctacccatg ctgttgatac ttgccttgcg gctctagaaa tagtagaact tattaaacaa
    42901 atcaaagaga tcaaatcttc attgaaccta ccttcatggg aaatccgaat cgggattcat
    42961 tctggatccg ttgtcggggg agtcattggc aaaacaaaat ttgcctttga tatttgggga
    43021 gacactgtaa atctagcaag ccgaatggaa tcatctggcg aagttgctcg agtcaatatt
    43081 tcagaaaaaa cataccatat cgtgaaagat ttttttgatt gtgaataccg tggagaattg
    43141 gaagcaaaaa ataaaggaaa ggtaaaaatg tatttcgtaa atcgtattaa accagaactt
    43201 tcagtggata acaatggcag agttccaaac gataaattca acctaatcta tgggagtttg
    43261 taatcgaagc attccctaat tcgattctcg cagattcact ttttttaaaa aaattatttt
    43321 ttttctcctt ttttccgcac aaagatagac ttttaattct cattatatat caaatggcaa
    43381 ctgccatcct taaactataa atcttaactg taattctaat tacgaatttc cattctaacc
    43441 ttttttcttg aatttaatcg atgaaattat tttttcccag caaaactatt tacctaatta
    43501 ggagatttca atgttacaaa ccttttccca atcaacaatt tcattcccta gatcaaattt
    43561 ccaactctct gattttagct ccactcttct tatccctgaa aaatacttat ccactctcca
    43621 attaaaatta attcaacaca aaggtcttcg cagctatgtc gcctatcttc tttccaagta
    43681 tcaacttttt attgcaaatg gcttgatccc tggttactcg aatgtaacaa ctaaatatca
    43741 agacaagggc caaaatcttc aaaagtttgc atttcgacca cgaccggaag attgggcaga
    43801 acttaagctc tatcgtatta gctttggtat gtctatctct gcctttttta tatacctact
    43861 attagcagac tctgtagact tagcaaaagc tacttcggac tacctagagg gtgtcggaat
    43921 ttcaatgatc ccgaattttg acttgagcgc aaaagtattt ctatggaata aaagaactta
    43981 ctatactact ctttttcact ttcggaagag aaaagaacat taaaaagaag ttccactaaa
    44041 caaaaccaat taattccagc tatcgttatt caggccaagg attctaagac aatctgaaag
    44101 gggataaatt tacttacaga aataattaga tatcaatcat agaagattga attaagatca
    44161 tacaatcttt aaatctaaaa tacttttcaa aaatatttag gaaataacgt ttccaatatt
    44221 tcatgtcctt ggtaggaaac attacagtat atttattgat ttaaggagga agttgtatat
    44281 ttatgttctt aagaattagt agagatatta tttaaatgag gttatcgtaa aataaaaagt
    44341 ttaatcatga ttaatcccga aattattaaa aaaattaaaa ctgcaaaggt agtatctgtt
    44401 ctaactggag caggaatttc tgcagagagt ggaataccaa cttttcgagg agcaggtgga
    44461 tattggaaaa attataaagc agaggagtta gctactccag aagcatttgc acgagatcca
    44521 aaattagttt gggagtggta tgcaatgcga atgaaaattt gtgcagaggc aaaaccaaat
    44581 gcagggcatg agattgttgc taaaatggaa acctatttcc ctgaattctt acttattact
    44641 caaaatgttg atggactaca taaaagagct ggcaataata agatagcaga gatacacgga
    44701 aatattttca ctgcacgatg tactgcatgt aaaaagcaat ttgcattgaa tgaaatcccc
    44761 tctaaatttc ctgttcattg tagtttttgt aaaaatttgg caagaccaca cattgtatgg
    44821 tttggagagt catacgactc taaactcttg caaaaagtaa tatcattttt agaaagaacc
    44881 gatttaattc taattattgg aacttctggt caagtgagcg ttcccataca attagcaatg
    44941 caagctatag ataacggtgc ctattcgata gaaataaacc cagaaaattc tacgatttca
    45001 aaccgagcgg atttacattt ttcgaatccc tctgggcaaa ttcttccaaa cttttggcaa
    45061 gaaattattg aaaattaaac ttgatgttta aacataaatt tagtttcatt cactaaagat
    45121 aaatttttcc ctattggagt ttctgatgcg atcctataca aagagtatat ttattatttt
    45181 tttggtttcc tttctctcag caatacatgc caagccttcc gaagataaag gaatcaaaaa
    45241 agcaatccga aagggagata ctgaattttt caaaaatata aaattcgaag atttaaaaga
    45301 aggaaataag cctaactcag aggggaaaac tctaattacc cgcatggaga tagatggtgt
    45361 aaatgcgtta gttggagctt ttaaaaaccc agatgttgaa gtcagaaaaa ttatatataa
    45421 agaattattg tatcctgaaa tttttaaaga tctagagggt gtcactgttg ctgaatttat
    45481 ggagcaagac aaacaactca aacaatactt agacaaactg agagttgcaa tttactctgt
    45541 ggatgaactt gaaaatcctg aagcaaatga atcgttaaat gctcttatta aacagtatgg
    45601 ttacagtgta aaagaagcga ttaaagttaa aaacccaaaa gcgttagcaa tcatgtctcc
    45661 tgattttttt aaaaaatcca atctagaaga ccctaaaact gtaattgtcc gattcaagaa
    45721 aaatggggtt aagactttag tgaaagcttt ggaatacgaa aaaggtaacc ccgaaactag
    45781 aaagttaatt attgaaagtc ttttttataa aacacttggt ggtcctgaaa aagtagtcac
    45841 taagaagaaa acgagagagt ctagggggga aaatttattc ctaatagctg ataaagagaa
    45901 gaatccagaa gttcgagcac aactcattca gcttgcagag gcatattatt ttgtagagga
    45961 cgaagcaaca gaatcatctg ttgaaaagaa aacagaaaat gccaaaacgg aatcttctaa
    46021 aacaaaggaa gacgatgaag aagaggaaga aatcaaagag gcaaaaaaat aaagacaaaa
    46081 gcaaaatcta agaaaaaaaa atcagaggaa taattgaatt atccaaataa tttcaaaaag
    46141 ggttttcttt cctatatagg attaggagag ttagccgctc aaggttcaag agcaatcctc
    46201 gcattctggc tcatcatttg tatgacattc tttctatttg gagatcaaaa cttaattgct
    46261 cccaatttaa aaaatattgc gaattcattc ggtataattg atcagaaaga tattgattgg
    46321 tatattggag gcttaatccc tatcttcttt tttatactag gtggactcat ttcaatctcc
    46381 atggggtatt tttctcaaaa attttcccgc aaaaatctac ttattttttc tgttctcatg
    46441 ggagaaattc cctgcttttt aactggattc tctaattcct acactgagtt ttttatttat
    46501 cgtgtcctat gtggatttgg tttaggtggt gtatttccaa ttctattttc tattgtgggt
    46561 gattatttct ctattagatc aagggtaact gcaaccgctt acatttctct tgctatgggt
    46621 ctgggtgtag gcatcggtca gttagttggt ggaatacttg gaaattcaga tccaatcaat
    46681 ggatggagaa caagttttat tgcaatgtca gcacctgctt tcctttttgt tttggtgtat
    46741 gcgatcttct gtaaagagcc taaacgcgga attatggaag acgaattcaa agacatcaat
    46801 gtagaagaac taaataatca cttatcatgg caagacgtaa aagttatcct aaagagcaag
    46861 acaaacttag gggtatttct gcaaggaatt ccaggctgtg ttccatgggg tgttttcttt
    46921 gtgttcctag ttgattatta tgaatcaacc tatttattaa gtaaagcaga agcttctgga
    46981 ttattaactt tcgcagcgat aggaattttt ttaggaacat ttcttggggg cgtcattgga
    47041 cagaggctat ttaatattaa ccacatttat caacctatct ttgcaatgac atcaatcttt
    47101 ttaggcgttt ttccatgtct ttatctactt catgcagata gtattgcacg atcagcactc
    47161 tttataccaa tgaatatttt tgcaggtatg atcataacat ttccactttc aaatgtgaga
    47221 tctatcttaa tcaatgcaaa tgcccctaaa aatagaagtg ctgcatttgc tctctataat
    47281 ttaacagacg atttaggaaa aggattagga cctgcactca gtgcgattat actaaatctg
    47341 attccagatc gaacgatggc tttatcaata tccatattat tttggattcc atgcgctata
    47401 ttttggttgc ctatcgtatt taattttaag aaagatgaaa tgaatgtaag agaaacatta
    47461 ttgaaggatg ctcagaggat taaggtaaca aaatgaaatt agaaaattat tctgcctgtt
    47521 tatttgatat agaaggaaca actactccaa ttagttttgt tcatgataaa ctatttccgt
    47581 attcaagaga taaaatggaa agttttctat tgtcacaaaa attagaacca catatcatca
    47641 ctgagttaat cgaagaaaac aagaaagact tttccgaggg aacattttca gctcccattt
    47701 taggatctga tcatgttgta aacttacaaa ttctagtctc ctatttgcat tatcttatac
    47761 gtgtagatcg aaagagtaaa cctttgaaag aaatacaggg aaaaatatgg aaaacaggct
    47821 atgaaaaagg agaacttaaa agtgaaatat ttgcagatgt tctcccattc ttcaaaaaat
    47881 tacacgaacg aaaaatacct atagcaattt attcctctgg aagtattgaa gcacaaaagt
    47941 atattttcca atattctaat tatggaaatt taacgaaata tatatcttat tattttgata
    48001 cgaatattgg caataaaaga gaatccaaaa gctatgtaga gatagcgagg caaataggta
    48061 ttaaccctaa tagagttctt tttttcacgg atattaagga agaagcaaat gcctgtcacc
    48121 aagtcggagt atctgcaatc attttagacc gtccaggaaa ccatctacaa gctcaacata
    48181 attttcctat cataactgat ttttatgagt tcatataata cacccgaaaa ttctaccgca
    48241 ttaaatcaat ttttaaacta taaaggtgaa gacttccaaa aactcatttt aattcccaag
    48301 gaagcgtcaa ttcgtaacta ttatcgtgtg atctatgaga accaagagtt agttttatgc
    48361 attgatgaaa attttaaaac aattccttat ccgtttttag atatacgtaa ctttttagaa
    48421 gagaattcta tacctgttcc taaaattctg aattatgatc caaatctcaa tgccatttta
    48481 atgaatgacg caggtgagaa agatttaaca agcattctag acgatagtga atatatctct
    48541 caattaaaaa tttctttaga gtatatttta aaactacaaa ccttaaatcc aatacctatc
    48601 attgctgata gatcctttca ttatgaaaaa ttaatgtttg aagtaaacca tacatgctcg
    48661 ggctatttac gatttaaaga aaaatataaa attgaattag acattacaag cgaaatgtat
    48721 gcatttctgc aagaagcttc aggattcctt gcaaagtttg aggaaaaagt aatttgtcat
    48781 agagattatc atgcaagaaa tatattactc aatataaaac atgaacaaac cattattgac
    48841 ttccaagata tgatgatggg aactcctcaa tacgatttag ctagtcttgt ctatgatgct
    48901 tatagacctc tatcaattca aagaagggaa gaactttatt ctttctttaa agaaagatct
    48961 caattcaaaa ataaacgatt tcgagagtat tatttaaccc aagcacttca aagatcattc
    49021 aaagcattag gcacctattt ggttcaattt aatgaaaaac aaaacttaaa atacaaagaa
    49081 agtataccga aagccttaga aaatttaatg gaaatatgtc aattgggaag gttccctgat
    49141 cagctatatg tatttttcta cttattaaac aagcggctaa cagagaatac cctctttaat
    49201 gaaagttaat taagtttaaa acgaattgga attaaaacct taacagtaat tgcttttcca
    49261 tctaagaatg atggagaaaa cttttttgag cgataaactt ttattgcctc ttcctctaaa
    49321 ccaaacccaa attttttccc aacggatctt acttgtaaaa cttctccagt atctgcaatt
    49381 acaacctcta gggtcattgc tccttctact ccagcagatc ttgcttcttc agtatactcc
    49441 ggtttttctg ttggacttaa gtccaccgga gcagtagctc ctgaaagaat tgcatcttgg
    49501 gctcctgcta ttcttggatc ttctttattt tcttttttca attgatccgt tagttccaca
    49561 tctccatcat tcggggtgtc ggccacattt tcagtaattg aaacagagtc tacaaatgct
    49621 acttcatcca caaatttatc gagtgaacta aattttaaat ccggcgtata ccaaaaaaac
    49681 aatatcaata actgaaacac agtagagatg cttaatccaa cgttcaaacg atttcgatct
    49741 ataaagcgat ggacgatagc ccttttagat cttctcggtt tatgctctag agaaggtgtt
    49801 tctttttttt catctacaat catttgagac atgctattgt cctttgagtc caccaccctg
    49861 ggtggtttga gttacaagag atatcttaag cgcaccagct tcttttaaca tatcaaaaac
    49921 atgatctact tccccatagg acaaatcttt atctgcatga attaaaactt ttaaatcagg
    49981 tgttgttgca agttttgctc tcacattatt gatcgcttcc gcaaggggaa ctcgcataga
    50041 attatagtaa acagttcgat ctttatcagc acttagaaag atgtttgata cttttttgtt
    50101 taactgctct cccccaggaa catcaggaag agagattggt aaatctggat ctgaatccaa
    50161 tacggaagtc accataaaaa acaccaacag taaaaatgca atatccgcca tagaactgac
    50221 tggtatagaa ggcgttgatt tatttcgttt aatcatttct ttttccttac actcacttgt
    50281 gtgaaacctt taagttgaac agcagacaaa gcatctaaca tgtttccata ctttgtttca
    50341 ccactagttg aaataattgc aagtttagta gagatatctt ggatctctaa ttgattcaat
    50401 tcctctctaa actctttaag agatttataa tattttgttc caacggaaac attccgcatt
    50461 gagacagtat ctcctgaaac gttaatctca aacacatttg atctcaaaac ttgtgttgga
    50521 ggaacatttt ttcttggtaa ctgaatattg agtccttctt ttacaaaaaa cactgctgtc
    50581 accatgaaaa atactaataa aagaaaagcg atatccgaca ttgatgccgc tgaaatttct
    50641 tccatttcaa ctttttttct tagtttaacc attttctttt acttggcaat taaagcgcct
    50701 ttctttctta ataactcttt atagatttta tttgctcctt cttcaacttc agcagcaaaa
    50761 ccatttactt ttccgctcaa gaattgataa aatgacatag ctggaatagc tactatcaat
    50821 ccggcagcag tagtaatcaa cgcttcttta ataccacctg caactacttt tgcattcact
    50881 tgatctgcgt tggcaatcgc atcaaacgca ttgatcatac cagacacagt tcccaagaat
    50941 ccaatgagtg gagcaatcgt tgaaacagag gctaatattg ttaaacctct ttctagcagt
    51001 gtaatcacct cgactgcttc cctttctaca cctttagcaa ataaatctgg atcaccgcta
    51061 gaaacgtcta caccatcgga tagaatctga gatattcttt gctcaccatt ttcttttaaa
    51121 tgagaaagag ctccctctaa acccttttct tcaatcttat cagaaagtat ttgattatag
    51181 ccttttgaaa ctagtttggt agtaaaaaag aaatataatc tttctaaagc gattccaaac
    51241 ccaatgatag aagacaaagc taaaggccac attgaccaac caccagctat aaataaatcc
    51301 acaagacctg gactctttgt aggagccttt gccttttctt ccgtgcctag tgatggatca
    51361 ttggtttttg gggaatcttt tgattcaacc tgtgttgggg ttgttggctg ctccgtagtg
    51421 ctttgtgcaa atatcggggc aaatagatat aggattgtaa gtagaattgt tgtaaaagca
    51481 attgatttaa ttgctgtttt tggaattaat agtttcattc aaatctccag ttttttctta
    51541 ttttaacttt ttgtcttaag atttcattac agcattgtta caaggttgtt aataatcttg
    51601 gattcgagga ttgcgaaaaa ctacttcaaa ataggtattt tctaagtagg aattaggcaa
    51661 aaacagaaca atcctgtaat gaaatcaaga agaggaaaaa ttttgcttta caaaaagaat
    51721 atttcatcta aaaagtcata gcacctatga gttctctcaa tattgaatct gttgacttcc
    51781 tcaggttgtt tcgagcaggt caaccagttg taattcagtt ccagaaattg gcaccgtccg
    51841 atatggaaca agtacgtaaa attacagaaa caatcttaga agagagaaat ctaaacaaat
    51901 tctttgcatt tgctagcaca gttatcaaag aattcctaca aaatggtgct aaagcaaccc
    51961 agaaacgaat cttttttaag aaccacggct tagacattga taaaaaccat aaacaaggta
    52021 tcgaaaagtt taaggaatcc atgtcaaatg gagatatcca taaccatcaa ctgattgatg
    52081 gaattgattt cttagttgaa ctaagacttc aagagttaga taaaggagtt ctaattagtg
    52141 ttcgtaatca cggcgaaatg aatgaaaatg aaatcaagaa tgtgaatttt atgattgata
    52201 gaggtaggag aacaaatgag gtatcagatt tactttctga tgagacctca aataaggaag
    52261 gagggggatt agggctcagt atgattgtta ttttaatgaa aaaacttgga cttacccctg
    52321 attgtctgta ttacgaagta aaaaattctt ttacttcttt caatgttaaa attccttaca
    52381 atatatagag attttttgga ggggaaatgg aactcaaagt aaaagatcac aacaaaacga
    52441 aaatattcca actagttgga agcttagata tttataccgc atctaaagtg aaaaaagaga
    52501 tcactcaaat tattgatgat gaagaggcag agtctgtagt tttagatatg tctaacgtaa
    52561 atcatatgga ctcttccgga atagcactcc ttgcaaacat tcaaaagaaa atgaagtcca
    52621 gcggaaataa gtttggattg cttgcgccta ccaatgatat aatggcagtg cttaagcttt
    52681 catctcttga ttcttttttt acaatctaca atactgaagc agaaattagg tagtgagagg
    52741 ctaaaacctt tcactcaact gattttatct tattattggc ttctcttttt aatttccaat
    52801 tttctaataa aatactagaa taaaattgaa tcgtattgta aatcttatca agacgataag
    52861 gtctaacaaa gattctccaa agccaggtta atccacgcaa ttgaaagtag tcaggtgcct
    52921 tcttaatttg cccagataga gtatctaaat taccccaaac accaatgaca atggacttac
    52981 caaaatatcc tgtattgttt tcaatccata cttcttgtcg agggaaatcc atcccgatga
    53041 tgataatatc tggatttgtt ttacgaattg cttcttttac cattaattct cttgcgtcat
    53101 tcaaataacc tgaatgtctt ccgacgattc gaatctctgg aaagtgacgt attaaattaa
    53161 agaacaatct ctctataata tcttccttgc tacctaaaaa gaagagtgtt aattcctttt
    53221 tctcggaata acgtattaaa tccataagta ggctaataat ggatatccgc tcttttaact
    53281 tgaatcccaa cttattagcc gcccactcaa ttccgccccc ttcagctata acaagacttg
    53341 cctttcgtgc aatacgattc atcttcttgc ctggctgcat cagcatgagt ttgattggat
    53401 caataaatag tatatgatga aaagaatcct ttttcttatg aaagtctaaa atgtgagcaa
    53461 tggcttcatc acgagtaata ttgtcaattt ctactcccaa tagagaaatt ctttcttttt
    53521 gtgttatatc tattttttgg tattctaata cataatctct ttcctctttg gaggaattat
    53581 gtgttatttc tttcggatct ttcatacagg tttagattct ctaacagggt gatgactatt
    53641 ccttataaag tcaacaatct ttactagact tttttcattc gatagcattc caatagatgc
    53701 aggcaaaata tttccaactt catagactat aaaaaaatta gataaatcaa taccaccaat
    53761 aggaacaaga ggaattcttg ttttgaaaag ggcttttttt aattcttcga tacccaaagt
    53821 tggatagtga ctttttttcg tttctgtttt aaacattggt ccaaatcctg tataatccca
    53881 aatttcagta tcaagagaaa ggatatcctc aagattatgg gaagaggttc cttttagaat
    53941 tgatttattc tctttcaaaa ctcttttttc ggaatcagac aaacggatgt aatcctcttt
    54001 tccaatatga atcccaaaag aagactcttt gatccctatc tcccaaaaat cattcactat
    54061 aattcttaca tcaggaaaaa aacgagaaac ttctaagtat aattttagat attcattttc
    54121 atttaaagac tttgctctga gctgaaaaaa aaatataata tcatcgaatc tattccaaac
    54181 ttcaattaaa tgaaataaat ctaggttctg ctttgcgcaa aaatcaaaat ctagaatagg
    54241 atataattta gcagatttta aaataatttg tttgttcaga tttaaccacc accaacgaat
    54301 tttattattt ctaaggtatc actgtctttt atataagtag actgccattt ttctctttct
    54361 aatatttctc cattccattc aatcgcaatc gtatttggat ttaatcgtat agaggtaaga
    54421 tattccgaaa tagtaaaact atttccggaa atatcaactt gttttccatt tactttcata
    54481 cactaacctt ttttagataa ttgacatcat gtgtaataat taaaaaatga aaatatgaat
    54541 cgattcgtca aatttttatt tatattgctt atacctttta tatttattca ttgccctact
    54601 catcctagca atccagataa aaccacaaga aatgctgtaa ccttatatta ttttaattgt
    54661 aaaggaggat cattagctgc ttgtaactcg ggatgtaacc ctcagtgcgg gatagctgat
    54721 ggagcagctg ttacaacaga taaattctct tgtcttacaa attgtcaaag cacatgttca
    54781 tctgattgca acataacgac aacgcttctt ttgtatttat caaaccatcc aataaaaaaa
    54841 taaattccat tgaatagact tggttttttt ttaatacttt tgagcctaat cacttgtagc
    54901 cgctcgaatg acagtaagaa taacaatgcc ttaatcatta gtcttctcta taataattac
    54961 aaaaatagtc agtccgatta caattgcaaa acatcaccgt cgccattaag ctttgcggat
    55021 tttagaacag ccattgatac tttaaatact tcaggatata aatgtagtga gtgtcatggt
    55081 gcaaacacag cacaagcaaa ttttattatt acgaattata gttctgtagg tgagagagtt
    55141 ttaccgggta acccgaaagg aagtatactc tatttcaagg ttcaacccgg tggttccatg
    55201 aatgcctatt ccaatgcgaa cgttaggcaa gcgatttatt gttggattgc aaatggtgca
    55261 aatccataaa taaaattatt cttcaactat tgtcacagtt tccattatca ctttttcaac
    55321 tggtgaatct tgaaaaccgg ttttcacttc tgatattttt aaaactagat cactaccatc
    55381 tataacatgc ccaaatacag cgtgtcgatt atctagataa taattatctt ttacattaat
    55441 aaagaattga gatcctcccg tattaggacc tgcatttgcc atagatattg taaactttgc
    55501 attttttaaa tcaggatgaa actcatcttt aattttatat cctggtccac cagtcccatt
    55561 tccttttgga catccacctt ggatcataaa attttttata atacgatgaa acgttagtcc
    55621 attataaaat ccttcttttg caagtttagc aaaattacca gcagttatag gcgctttatc
    55681 cattgctagc tccaaagtaa aagagccatg atttgtttga aaaattgctt ttgtcatttt
    55741 aacctcagtt atccagattt gaatctttga ggttatactc aactaaaata ttgtaggttt
    55801 ctgcgaagga taataactag atttttcaga atgaaacttt ctcttctttt gggttgcctg
    55861 tgatagaaat ttcatttaaa aaaagtttcc tttggtctaa acacttctta aagtttctaa
    55921 tgcgactttg tcaagtcgcg cacgtttgta aaatagaatt gaagtggtca ta
//
LOCUS       DAOQOS010000017        55593 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_213_length_55593_cov_6.422540, whole genome shotgun sequence.
ACCESSION   DAOQOS010000017 DAOQOS010000000
VERSION     DAOQOS010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 55593)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 55593)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55593
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_213_length_55593_cov_6.422540"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(106..963)
                     /locus_tag="PLF66_05625"
     CDS             complement(106..963)
                     /locus_tag="PLF66_05625"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: protein motif:HMM:NF015290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class II"
                     /protein_id="HMZ63554.1"
                     /translation="MKIKNCDELDSIHLNKIMNLNAEYILNTLANITEKTAQKTFPLF
                     GKMDKKKADHIAVEEMRFLLGQLPFQSRVIIGEGEKDEAPMLYEDEILGGSEFQIDIA
                     VDPLECTTNFAKGLPNSMCVIAFTERDGLHRVPGTYMEQWVAPPQMKYPFEPKENIKA
                     NLEKLSDSLHKNISDLIIVVQDRPRHKELIESIRSLGCGISLIESGSLTCIMDICLGK
                     GNYDALIGTYGAPEGLIGAVIAKATGSEFKGIIRAHEEKHREKWIATGREEEEILDKN
                     DSDSKIDIK"
     gene            1221..1769
                     /locus_tag="PLF66_05630"
     CDS             1221..1769
                     /locus_tag="PLF66_05630"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HMZ63555.1"
                     /translation="MITKIAPEYESSKEVMDLWKGFRKEVIDCLRDVPTDELIKSPEG
                     IWSLSEVAEHLYITQWNIARSVPIVMSGRGNSVFNGYTKEPDYRMIRLSLSKPSGVKN
                     PDSVAPLNKYSFAELLPLLNKAESKLEDSTNKYSKADLMKYKMEHPYFGDLHLFDFLW
                     VMSWHEHSHLYAIKKRTKELRG"
     gene            1817..2443
                     /locus_tag="PLF66_05635"
     CDS             1817..2443
                     /locus_tag="PLF66_05635"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020776931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HMZ63556.1"
                     /translation="MTLFLPLFYFVTLTSVPEPTLRAYFYSYPGKLSLVNWILASCFH
                     SSLSHLISNMGFLYFLGRAVEAKVGKGKWLLFYGMAAIISMMADSIVRGYIIVDRNIP
                     TVGASGAISGLAAIAALLSPVTYRVNGVSFPFPVFLVAWIMVYTDIMRAFSSDLIAHW
                     AHLAGFFSVFITTYLLSPADQAKIRNGFLFNFTFFILTVILIYLLENR"
     gene            2448..3851
                     /locus_tag="PLF66_05640"
     CDS             2448..3851
                     /locus_tag="PLF66_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002758443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HMZ63557.1"
                     /translation="MHIKFNSLEYTADDQFIESEYEYTGDKSSGWEIKRNGKAYLKLG
                     QGYELLKTKLCGVCSTDIARRFLPFPLPQITGHELVAETLDGKDHYVVEINDTPAARG
                     DAVQDEFCIAGIPTHSPTRLVLGIDRLPGGFGPYILAPQNAIVPYKKISDKTAVLIEP
                     FAAGLQAVIASPPKKGDRVAVLGPRRLGSLLIAALKAYKESYQIDFSITALARHEELL
                     ILAKKLGADQGINLKEVSLESLYKSFDIVYDTTSTESGFLSALRFAKREVHLKSTNGL
                     EMCGFKKLTELVVDELSILPFSNENLNFKWEKEDRKNERIYSISPISLDSKKFKLYQT
                     NPKQAEEILASEEFAHLVPRFDLGIATSKEEIDLMIRPNSSHENSLIRPRGAILFKGE
                     NQNNPLLSFVNEGGAIRTSRCGDFHLAIRLLEENQALAESLSENMISHVYSADKMNEA
                     FVKAKDSSAIKVIVEHL"
     gene            3848..6277
                     /locus_tag="PLF66_05645"
     CDS             3848..6277
                     /locus_tag="PLF66_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003007768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63558.1"
                     /translation="MRTIDTSLGFPLIEDDKCWKIHLEELRLFTGLSVLARILGDQIL
                     DHMETQFSDISFSYKINPNINPELLQMQIVAIQVYAKSGELDDIIFHIDEFHSHLRTI
                     FGTFQRPKWAKRIHPEFYGEDPREVVSSPLLFPLELPSSKKNLLFVLERVANSRYTGK
                     FFMRLSIETFSDVLSIPPGKGTPVDDLDARVYIAGTTKLSESISDKILTASNKGLVAY
                     SEENTPYSSLFEQLHKTHLEKVDQINFYWNRDFSELVQDITKEDQVLIFKKIFLSLED
                     ESVCRLLQEGETIRLELGLTFVNLYLTRLSRVLNFSLGLEKKDFSLDYYLKRMPLLEK
                     LTDTSQHGKDFSNIRIFLIHHITSEIIAVIDALRKLKVDALDVIFVKYAGVIPSKYLD
                     VLLDISDKNFFMAGLTRHLTETNKEYFTPAKYYTDISSHVNLQKTLQEKKLGFFEAMK
                     YLSCYFFLLFLNKSIQEGKKVLIIEDGGYLSPLLNEYALSEKLTKDVYNSYQITDIPK
                     VESFRDLLKENLVGSVEHTRNGYDKLAFVKEKFGKLNHIAYSIAISKNKVVEESKEVA
                     HSILSAIESILHGQGMVLSSKKTIILGAMGNIGSFLTKYMKEGRLHDTNKELIGVDIR
                     YPQVTKENYKALQEIPDEIFLSLELFIGVIGASIFKREFLSKIILQGTKERLIFASGS
                     TKTVEFTDLSEYLYEISTMESPSIDGIPVRIVFDKIIDPQTDTVQGTKVFISFQKNSL
                     EITKTLYLLGDLSPINFLFYGVPTETMDRIISQLTSVSLGMVDQYKKGILPEPNLYAV
                     DHQIDEWGNTL"
     gene            6274..6696
                     /locus_tag="PLF66_05650"
     CDS             6274..6696
                     /locus_tag="PLF66_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020776015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HMZ63559.1"
                     /translation="MSKHGLFQITQKVFIRKDDLLLVMKDKKSGAGDLPGGRMNQDEF
                     FGDWMDSLKRELEEELGSEFQVDISPKPILVHKHLVNDGNHPCVIVGYSGVYRSGEIS
                     ISDEHDFFKWVDVKTYQPDELFSEYMLDAVKLYLKEFA"
     gene            6693..7814
                     /locus_tag="PLF66_05655"
     CDS             6693..7814
                     /locus_tag="PLF66_05655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63560.1"
                     /translation="MNRLFRYILFFSFAIVSFYFAWSQGGRQINESEYESLSSSIPPL
                     IIRSLGYDRNNGNIVGIQPIINPMDYSSEERFYDKIDSYLHTAKKRGFLNENTIVAFP
                     EHIGTGLVLLGEKSSVYSSSSLTIAFTEMAFSNLLSYIGTSFTKTHDSQTELVFKMKS
                     EEMRNSYVETFKRLSSVYNVTILAGSILLPEPEIDKTKNTIQLKNGKLQNASFIFLPD
                     GKIYDQIGIKKQLAVSEGQISIPRKAEMGNLKYKNILILFSNDSLYNSIYGETGKDTE
                     IVLVIAAILQGDSLDWKLEDINGAKEPDLGLTQAELWRKYNVFDKTRNSLSNKITMQI
                     VLLGKFFDMILEGETSALIRYVATEGIDNKSPAILNVWF"
     gene            7854..8042
                     /locus_tag="PLF66_05660"
     CDS             7854..8042
                     /locus_tag="PLF66_05660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63561.1"
                     /translation="MTEIISQKELNIHCPYYLKNIKSCPSAKKILFPEDMERLEGCCL
                     SETYSSCSIYRELIEKAA"
     gene            complement(8185..9294)
                     /locus_tag="PLF66_05665"
     CDS             complement(8185..9294)
                     /locus_tag="PLF66_05665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63562.1"
                     /translation="MKYLPLILLAILLNCDGKNESAVKDIDTLIEDEKFSQAQDKIKN
                     KLETKRASDEILSQKKPINPRIIEMSNDRNRIVWTEDKQITFRDLANPLSKSLTFPDV
                     PEMVSVSSEAEYAIVLFKLPNGAGCRMVNVSLIEPKPSYLSDTYVSCKNRTAISSDGS
                     SIYYFINENLYEESLKDPKKPKLLVPKEKFDSTYPNIKNRTHIFSIAKTFLIFFGNAG
                     VYNLYWFNPKDNSITKLATEIVTPKLYYGNGKNAFVLSGVVGSLTLREIKYTAYGEPN
                     IGKGFPITYNEINPFPTTKANEFISGKSGSIYRWGPNVSKKLYPILAEKFWVVARDFI
                     LYENNSQELILTSTEFSPEEWKLLELYKQVKQKNK"
     gene            complement(9291..9887)
                     /locus_tag="PLF66_05670"
     CDS             complement(9291..9887)
                     /locus_tag="PLF66_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015676767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63563.1"
                     /translation="MAQDKFTERAFELITGVEAYTSPYAETIGDPAEIAEELTQAAAF
                     KTAAISAGLSLPTGLFSIITILPELFMIYRIQGHLIKDIASLYGKESLVTKELLIYCM
                     FKQTSLHLVERLLEDVGTKIIIRPTTIRFLESLLQKIGMNLSKRMIRSQLLKWLPLLG
                     AALTGGLTYLDTKYVAATAIGLFAKDILIESGELKQSE"
     gene            10184..11251
                     /gene="prfA"
                     /locus_tag="PLF66_05675"
     CDS             10184..11251
                     /gene="prfA"
                     /locus_tag="PLF66_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HMZ63564.1"
                     /translation="MKDRLLKIQQKFIKIDEELSSPNLNSQKLKELSKERSRLTPIYN
                     KIEEYFKTEKNLEDAKALIESETDSEMIGMLKAELEESNSLLSALTQELEVMLLPPDP
                     NSGKNILVEIRAGTGGEEAALFAADLFRMYTKYAEKKRMKYDIIDMTPTGLGGYKEII
                     FSIEDERAYDLFKFEPGAHRVQRIPTTEAGGRIHTSAVTVAVLPEAEEAEIKIQENEI
                     RVDVFRSSGPGGQSVNTTDSAVRITHIPTGIVVSCQDEKSQLKNKDKAMRILRARILE
                     KQEEEAKASSDALKKQMIGSGDRSERIRTYNFPQERLTDHRINYTSHNLSAILNGDME
                     DLVNELIQADRIQRLQESKTV"
     gene            complement(11497..14064)
                     /locus_tag="PLF66_05680"
     CDS             complement(11497..14064)
                     /locus_tag="PLF66_05680"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HMZ63565.1"
                     /translation="MNQKYIRYLGYGLMIALSHDLMIAQETSPQKTPADPKQKEAILF
                     YWKSEVTDLMEQKKALDAKYTELQTKYTKDTEALKAENERLKKLNAELEADKQNRVAS
                     RDTQTQNADQRIASLQQELAETKRQKEQLERDLAAKTFAEQQAQERIKTLERSIAAAN
                     AEKNATVASNQNNSDLQAQVNSLTAQRDQLQKDLAANNAYIDSLKKNQNTTTTTTTST
                     STSSQGYVINSVTPSGASTESLLLDSQAQVKSLEARLIDVIQERDRLQKDLADAKAGQ
                     LQAVRPETPATVPSNYAPAAPKETTKTTVTTSTESSTTTTTSASKSAAAAAATTTPTL
                     APVASTTDQDKRIADLQAQVNSLTNQRDQLQRDIAAIYGYQTPTVGQPANPTTVIENN
                     SKTAAGTTANTTTSTSTPTVISSNQSLDDAMARNKKLEADLAEMTKQRDKLQKELDTN
                     YNNMYNSKTNPLSPANPQTKETTTTTTTTTETSTSTAKSPAGSEAKLADSKDAKSVDA
                     KSADAKTADAKVADAKSSSTTTTTTTTETTSTTGITRIAQLENEVAALTKERDQLKRD
                     LQASKADKTIETRLAEVNSRIEKIQADKTLTEAEKAEKLKAANAEIEKINQEKNANTT
                     SIASNNKELEKKNAAMEDMKKEYESKIEALNKEIAELKEDKETLESDLATSKEQLEEE
                     GSKHKEEIERLTEQAQKLEEALEKEIKKGNKSKSNSGSNTTPASAQVSSKGGKTIISL
                     ASSVNFQTGSRELTPEGKRTLDNIIKVLKKHSTEKILVEGNTDNRPVPTNSKMRDNWH
                     LSFERALTVLKYLKKGQLQKTQFLAGGFADSNPVKPNTSESNRAANRRVDIIVLPKR"
     gene            14219..15553
                     /locus_tag="PLF66_05685"
     CDS             14219..15553
                     /locus_tag="PLF66_05685"
                     /inference="COORDINATES: protein motif:HMM:NF014543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63566.1"
                     /translation="MNKQSDLLNINLSFILLLILLTTYPIYSDECISGNCQNGLSTMF
                     FSNGDKYTGNFKNGKQNGKGMMTFAPRTDASGNFIYASKYSGEFKEGRFHGKGMMYFY
                     NGSQYEGEFKDDRFNGYGTYVFPNGNKYEGEFRDDLKHGKGTLTYAGGGKYVGEFKYD
                     KFDGKGNFVYPNGDVYDGEFQNELKHGRGTYTYTDGRIYSGGFKEDKRHGQGTYIYGG
                     GIKYTGEFVEDKFQGKGTIVYTNGVTYTGEFKDYKFHGKGSITYPTGSKYTGDFRDGK
                     PEGEGTYIFNTGKKYVGGFRDGKFEGEGVLRYANGDEYIGGFKNGKFEGQGKITKKNG
                     ETFVVEFKDGLFNGQGTMKYANGDRYVGEFKDGKKNGQGTMKFKDGAVYEGGFRNDKS
                     HGKGVLQFAEGDKYVGDFENGNFNGIGTMYDKDGNIIYTGKWINGDPEGGMK"
     gene            15550..16110
                     /locus_tag="PLF66_05690"
     CDS             15550..16110
                     /locus_tag="PLF66_05690"
                     /inference="COORDINATES: protein motif:HMM:NF014543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63567.1"
                     /translation="MKKIFLLSSIFALISTSIYAGDCVSGNCVNGQGIMTFANGDKYI
                     GEFKTERKSGYGVYHFSNGDRYEGDFKDDYPDGYGKAYLANGNYYEGEFKNNFPNGKG
                     TMRYHNGNVYIGEWKQGLKDGFGTMTYENGSKYVGEWRNGKKHGQGSYTQATATNGGE
                     IKKPQKTLETRMTGEKKSTPFWSGTK"
     gene            16134..17477
                     /locus_tag="PLF66_05695"
     CDS             16134..17477
                     /locus_tag="PLF66_05695"
                     /inference="COORDINATES: protein motif:HMM:NF014543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63568.1"
                     /translation="MRTKFFHLVLSMFLTTGHLLSSECVSGNCNDGQGTMAYSPTEKY
                     IGEWKGGQKNGKGIYIFGNGDKYDGEWKNGSFFGQGIFTFADGGKYIGQFKGNSFQGY
                     GTFYYPDGSKYAGHYSNGKRNGFGTYTYPDGAEYAGEFKDEKFHGRGKYKYADGDVYV
                     GEFKDDRFHGVGVLTLKNGDRYEGEFKFGLKEGAGTYVFANASKYVGEFHKDKYHGQG
                     TYYYSDGDKYEGSFKDDKFSGDGIYTFVNGTQYIGKFDNDKYSGQGKVNYSDGNKYTG
                     EFKDDKYHGKGSLVYANGDSYVGEFKDGEYYGKATLTYANGDKYVGEFENGRRNGKGT
                     YFYANGDKYIGDFKDDKYQGKGLYRFADGSQYKGDFRDDKYNGYGVYLYTSGDKYYGE
                     FIDDLRNGKGIYILANGTKYEGEYKNNKKDGMGVLYDRNGKVIFSGQWVNGEPSR"
     gene            17537..19381
                     /locus_tag="PLF66_05700"
     CDS             17537..19381
                     /locus_tag="PLF66_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000929340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HMZ63569.1"
                     /translation="MTKFNNQDIGNGLMVKPEDIYIYPVSEPIYKFIEKVWSSFVNKM
                     VGLVFANDKHMFDHAIFEVIDGTELKIVATATHFKMKEMIGKKSSKDIEEFMTKTKPI
                     SIFDEGNDSARIIQKAILAFSHSKRPDVEIINLRDSRIHPNLRVLLTSTMRYSVGIPL
                     IVKENPIGILWGIRKEKLTEAQKHEITMQLHTLFDVIEYVVARDADNNGDPYFARKNI
                     EKSDTTSSIHHLLYTVQKIQKDPVTSIIAHSHKYNVMYRLDASYIVPTAGGYSVSLKH
                     FARENLNDTDKIILMIPGFFCRRSVMDKLAREMALRYGYRVFAMDMRGRSKHTIPKDI
                     TSKVWTLDDYIQDDFPAVLNWLSHNYPNKKIVVMGHSMGGMIPRFYTGAYEKLKKLPG
                     KANLPNPYDHIAGIVSITSPNYMNLESQILGLDVIKSGVKLISNKAIYDTLFNLVSFP
                     VHTTVNSIDLNKFFRFILNFHSSLRQFSFSVSSKIVNLNDFIGYKQITPPEWYFFVED
                     VFCEESIKVLLQFLRSQLSHEKSFWSYDGNINYTQEQNDNFELPIFSVIGNLDKIVPA
                     ETIEDVLNLPKPKNKKVARYDQGHLGIIFHHDTVMDITKKADEWIKDL"
     gene            19390..20010
                     /locus_tag="PLF66_05705"
     CDS             19390..20010
                     /locus_tag="PLF66_05705"
                     /inference="COORDINATES: protein motif:HMM:NF016214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HMZ63570.1"
                     /translation="MALFITHGFFASLFSFLWRDKKTKFSVLLLILLASIIPDIDFIF
                     DDRNSGMFYHRGITHSIFFSAAIGVLFSAFFMSSFKSFGQTLLLMFIFFLAGLSHSAL
                     DLLTDAPNGVCLLCPFSEEKYSSPFTLFHSYTAGIAPKGFRKGVNSAWSSMIPEIFFV
                     WIPTLFVFFFSAYLVNKTTPVKKQTTYKPVQIKPKETKKTNYPKKK"
     gene            complement(20011..21090)
                     /locus_tag="PLF66_05710"
     CDS             complement(20011..21090)
                     /locus_tag="PLF66_05710"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HMZ63571.1"
                     /translation="MLPHEFADFFIQFGTGLCFLLALLEISKKEKSIAIGNLFFIGFF
                     LGLIQLRLGFYSTKLILKYPNLTFSFVPSILAIGPMVLKLGVTVTDFTSDKKVLLRLH
                     LIPAWIALFLDTAFVLLNPEQKISIVEQGLFTKSITPYKLLYIFAMLHILTYFGYMVH
                     MFLRITKQYEINYTSFVWIILNVPIAGVLFISIGFLNQIDSIYKLGVCILTITIILIY
                     VFNTKYPRFFISLQNDIKQKKYETTLLTEVNVDVVCKRLDELMEENKIYRDEELRLNS
                     LAEELSLTPHQLSRILNETYKKNFNEFINGYRIKEAEQILLSDPKRSILSIAYEVGFN
                     SKGTFNSHFLKITGLRPSEYREKNK"
     gene            21249..22097
                     /locus_tag="PLF66_05715"
     CDS             21249..22097
                     /locus_tag="PLF66_05715"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HMZ63572.1"
                     /translation="MANDKTIKVRHVDFGLQKELPNNYFKGNYFITHFVNSFHLIFPD
                     GEKYFIRAVKSHEDYVNDPELKKRIKAFIAQEVTHGNEHKKFWESLEKKGYDINTFLN
                     FYNSSSYGWLEPNLEKIFGTKHALAVTVALEHYTASIAEFAFNNLKELEGVPEDMKKM
                     LLWHASEEIEHKSVAFDVLQEVDNSYLLRMRGLIHATATLSFYTLVGFAFFMLQEMKK
                     GTWISPIEFWDFLVHFLDLAKTLGKEIANYMRPDFHPDQVENNHLAVYFFEKYHVEKA
                     NLKLAV"
     gene            complement(22136..22576)
                     /locus_tag="PLF66_05720"
     CDS             complement(22136..22576)
                     /locus_tag="PLF66_05720"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HMZ63573.1"
                     /translation="MNKKIVDSVLIVDDDSLEFFIAKKLLESTKCFKSIYSAANGKEA
                     LHLFQNYEESTQKHPNAFPPSLILLDINMPAMNGFEFLEEYQSFTTAKSELPIPIIIM
                     FTSSDEEIDKNRATKFPIVRDYLVKPLTFEKALKLADIYGTPTE"
     gene            complement(22573..23649)
                     /locus_tag="PLF66_05725"
     CDS             complement(22573..23649)
                     /locus_tag="PLF66_05725"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HMZ63574.1"
                     /translation="MKPREQDDSFKIMADNAPVMIWIAETNKLCTYFNKPWLTFTGRT
                     LEQEYGNGWAEGVHPDDMDRCLNIYITAFDKRESFRMQYRLRRHDGEYRWILDTGNPW
                     NDNDGNFAGYAGSCIDITEMVESKRLLEKANQELLEFNYRLSHDLVAPIKTVRGLLEL
                     SAISIKDQDWTNLTDYHDKIKQPIQKLEMLIQNILELSKVDALDQNPESLNLQEIISK
                     IQNSFQQELDNKKIEIEIELEANLITTYPSRLKEIIKKILENSIQFYDPNKEKHWIKI
                     HSKYDIKNNLIVTIADNGIGFDSEYSERIFDMFFRAHSLQVNGNGLGLYVVKKHIEKM
                     EGSIKVLSSRNDTIFQVILPNLKI"
     gene            24021..25058
                     /locus_tag="PLF66_05730"
     CDS             24021..25058
                     /locus_tag="PLF66_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002975137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63575.1"
                     /translation="MPRNNVNATGSSIPTATPLSTSKDITAYSIPSLGITGIINGTQI
                     SLASDTLTSFSPLVARFTTTGKTVTVAGINQVSDVTSNSYSSNLTYVVTAEDGSTKNY
                     TVTLTAPRTYGGGSLVIWLKADSLALSDGALVSTWPDMSGYGNHFTQATASKQPTYRT
                     NQVNGLPALQFRQATIQNMVISSGGVNLYIDDNASFFFVFKLIQTNAAGTTILNINGS
                     NGREMALTDPSGQYLQCRNGVSCSQTSGAVIDRSSFVAIGSIQIQGATVNEIWNGDLK
                     GVIAATGGNFNYVGGASPGTSYLPNGNLDADFAEVLFFNTALSQTEIDKIFCYLRAKY
                     NLISTNTSCGI"
     gene            25060..26106
                     /locus_tag="PLF66_05735"
     CDS             25060..26106
                     /locus_tag="PLF66_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002976934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2804 domain-containing protein"
                     /protein_id="HMZ63576.1"
                     /translation="MNNQQIEITSSIPLHDEEGYLSKEGWARSPYWQYDRSNLKASKF
                     RIKEWDYYSVLSPDGEFGITFTMSDLGYAGLFAICFLDFKKKTFQQVDTLSILPLGKT
                     NFPSKNNIGQVSFSDKKLSLKFTMTKGIRTLEFSSPLFKEEEGGKGLEGTIVLTEPED
                     LESMTIATSWKENRRAFYYNTKINCMPASGGFRFGNKTYSFDPKKNFGALDWGRGVWT
                     YKNRWYWSSGSGLIGEKPFGFNLGYGFSDRSPASENVLIYENKIHKLDLVSFQIDTKN
                     YLAPWKFTSNDNRLDLDFTPIVDRSSCLNLGILKSIQHQVFGLFNGSVFLDSGERVEL
                     KNFLGFAEDVLNHY"
     gene            26310..28751
                     /locus_tag="PLF66_05740"
     CDS             26310..28751
                     /locus_tag="PLF66_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004779012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HMZ63577.1"
                     /translation="MNKKSIRTSVIILLTLALLSGGFFGYILSEVDRGEELTKLATYL
                     PTTPTKLYDLNGIPFAELYRHRQELLRYQDIPPHVIHAFISVEDNNFYNHFGIDFKAI
                     IRAGFINLIHLKVKQGGSTITQQLSKAILKNSKKSFTRKFIEALLTLQIEQEYSKEEI
                     LEIYFNLVYLGHGSTGLSTASSVYFSKDVQDLDVAEAAMLARLPKAPVQYSPYKNPQA
                     AKKAHLTVLRLMSENGFLKKESIQAMHEEFWQRYWPIVITQSPSRSTWGTKLDRAPYF
                     TEHVRKQLLADLGEDLVYTGGLKVYTTLDLRKQEIAEDEVKKALTHYDKTSFGLSNSF
                     KAGVDGQLVGLYGLLGSIFPIAPMEISKFDEAANYRNTLENELSDALDILTAMTPIEN
                     EASAIGEFRKRTAVFTKNLHVEGAFITIEPGTGYIQTMVGGSKFTPKNQFNRAMQARR
                     QTGSSFKPYVYGAAINERVISSGTGIMDAPLTTLNDEGHGWAPEDSTGDFKGMVPAYR
                     ALALSLNIVSVQIFFKVGGPAIIDFASKLTKANKRRFPDNPTLALGVAELTPYEMALG
                     YSIISNKGRDVIPFSVRYVKNQTGQIVFNKELIVQKELAAKVANGTIQVIPESTAWIV
                     RKMLQGVADSGTPTTALREAKYNGVSGGKTGSTSSFTNVWYCGFDPKLTSVFWMGYDK
                     NSISLGPGMTAALTAVPVWGRIYARWYENQEYPQFKNADGADPMPEGVAAGGVCAHNG
                     LSPKPGVCPVGGALYLKPAMVDGVMRSVPWNRQCDGDRDHDKAIDFREFLQDEYKISD
                     EEIEKNKKPFKIKAD"
     gene            28751..29998
                     /locus_tag="PLF66_05745"
     CDS             28751..29998
                     /locus_tag="PLF66_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HMZ63578.1"
                     /translation="MKDDNYAVLRILDFRNYVIARFLAVVGIQVQAVVVGWQVFDKTN
                     DPLALGLVGLFEIIPAISVALFAGHLADLKDRKTIVLICYFVLLFCSASLLLLSTSIL
                     NLPANNVYPIYGIIFISGIARGFLNPTLAAFMAQLVPKHYYPNSSAWNSIAWQVAAVC
                     GPALGGILYGFNGPILAYAVDASLLIAAIVFYMRILPKPLPERKQGESIFESLFTGFR
                     FVFSNQVMLAAMSLDMFAVLFGGAVALLPVFAKDILNVGPEGLGILRASPAFGASIMA
                     ILLAHKPPRENAGQILLIAVAGFGVCMLLFGISSNFYLSVFLLGMSGVFDSVSVVLRS
                     TIMQTFTPETMRGRVASVNSIFIGSSNELGAFESGVTAKIMGAIPSVVFGGIMTMLIV
                     MITGAFAPKLRKLNFKEENSDSK"
     gene            29985..30878
                     /locus_tag="PLF66_05750"
     CDS             29985..30878
                     /locus_tag="PLF66_05750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63579.1"
                     /translation="MIPSSDLKEINSFLKGLNGSNAPFDIYVDNLHSPYVLLREEYLL
                     PPISNMDFTKKDILPFLKVISKYIPEAMVGCSVLPIQKPKRETGKISLVREISLRGHN
                     YLYIFKIDAAYLGGSAKDKIISAASQVSHPSFSTDRIYFSTKIVPIKNVIRADGEIID
                     FEVQTFDKGLFITELEETIDDRPKKYSELFDEIDYSPVMEPIKLNLKIMHPNWSLGKI
                     YEPVYIEYLTLSVRFLLASYANTIKHFSHFYEILEIIHINGSISEITRRNFIYWLSKH
                     SFERGTSPSGNMRWKILQDQI"
     gene            30883..31623
                     /locus_tag="PLF66_05755"
     CDS             30883..31623
                     /locus_tag="PLF66_05755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63580.1"
                     /translation="MKKILLGIILSIPLFAQRADFYDTYNYDMRNMKLGDSKDGVYRD
                     YDMPPKFQKPPLVNPKTVQRQPDISSLLQGQTSIRSSNNQSQNPVASQTNNLPIDPVT
                     GKINPDAIARQREKEKQNKEKKRQFLNREIEPYTETRGRRMEIIFLTTFPFAAAFSVG
                     VTVVIGSIMGNNTYIKTTPGFFMAILGATGLSAANMISDINSYDEYMKNKKETNPLPP
                     PPPEETQSQIYKGQPNYNFSFVFGAKNF"
     gene            complement(31959..32468)
                     /locus_tag="PLF66_05760"
     CDS             complement(31959..32468)
                     /locus_tag="PLF66_05760"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HMZ63581.1"
                     /translation="MNDHRVESLDALLIDIGLPHMNGIDLLKLIHSRYPKLKIIIITA
                     FNADDMIFNALKFGASGYIWKNEIKNIKDSVLAILEGGSYMSPSIGIHISQYFRSQVK
                     ENSDSSLSTREKQILELIITGRSVTQISKLLSVSEGTIRKHINNIYSKLEVSNRVQLM
                     QKARGMGYV"
     gene            complement(32543..33484)
                     /locus_tag="PLF66_05765"
     CDS             complement(32543..33484)
                     /locus_tag="PLF66_05765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63582.1"
                     /translation="MEIQNQNQTIEIDSFQVIGLPEKIHGINTTRQFLVSLSTEEFID
                     LVKKNQQFPSFQQWMNYYLWLFRDDSDFQQILSSPLFPTKFLIQFIYYALGRWIAVED
                     NDPDEFFMDIATLFSPEKCLQILIEENIVDLDVNLALAFISNLDENGINVYFEQTGNR
                     EDETNFFYDLFSELEDTMIKSFFIKNPELYNYILQMFKKEKDRSELHQKKYERFVEKF
                     KTDLDIIDLTLSIKEKVHTIFDLEEEKKKPCSQRNLKRIIGIINALKGKDNISDIVQI
                     LYRQKTIIDREEKDLIRNILTDDFLKSIFKLSDNIQL"
     gene            33608..35449
                     /locus_tag="PLF66_05770"
     CDS             33608..35449
                     /locus_tag="PLF66_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010572714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HMZ63583.1"
                     /translation="MFENRNRDKRKLIVLLYRIKNKFLDTRNSIHRFHYTYTRNITRG
                     IFALCGFLSIATLTIEYGFGYNPKWGSTIWLINYLAIQYLLAYEILGLVFTTDQYKNY
                     IKNHKPEIIIVSLVLLQKIFEQDIIYYLKLGEFGTNDTALLFLSINQIFLVFSNLARL
                     VRNTRLYNLKKLNPSLIFFFSFASIGLIGTLLLSLPKAQKIEGSLRLIDIIFTVVSAT
                     CVTGLSTINIFDSFTTMGQVIILILIQVGGLGLITLTTFFSIFLAGQASVNDKLLMKD
                     LLSEEAIGRVKRIIRQIAIQTLIIEAVGAELLYTYLPEDADLKGGNKIFFSIFHSVSA
                     FCNAGFSLFTNGLAENFFLHGKIYLVVIMVLITFGGLGFPVVSEVSKKLFTPDSSHIR
                     LSVSSKLVMVVSFLLFLSGTLAYYFLESKHTLKELDQFDRVFHSIFYSITCRTAGFNT
                     LDIGRMGVPMVFFSFLLMWVGASPNSTGGGIKTSTFAVSLLHIVDLVRGKNKLEIFNR
                     TISPGSISRASATIVLSLFVIFIAIFTMILIEDFPFLDICYEVVSAFGTVGLSRGITS
                     NLSTTSKIVISIVMFMGRVGVLTILIAFTPKSKAYNYTYPVEYVVVG"
     gene            complement(35871..39656)
                     /locus_tag="PLF66_05775"
     CDS             complement(35871..39656)
                     /locus_tag="PLF66_05775"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HMZ63584.1"
                     /translation="MKNDTNKGKVSYTRILFYLLLALAVAFSAGCGKKKKKAFFWMAA
                     LTGGNSNVGTGTGSGSSSQTDSNGVPLPSNSSDPSTAPTQEVATHGPATISGTLNATN
                     CKNASNVAIACSSDSGLDLTQITIQLVDSNGNVIATTTPDSTGKYTFNIADLSNGDYR
                     VLINTGNGLNYAYQDVNFTYNPVNPSANTLTGIDLTSSRLYLTTGPATITGTATTPGF
                     KDQNGNTIVAAGPMPNGTVVQLKDANGNVIATTTTTSGAYTFNIPNLPNGNYSITVIG
                     SGQSSNGQPFSDVSSAVPIQFSFQGNNPATPTQVTIPGLSSAWNPATSATANISNWSI
                     RNVAISGSDLSGFTVKLKDANGNVVGTTTTNASGQYSFSQTLSSGVYSVEISKAGFMT
                     TSNSFSFTANPNGSATSITQASPNNVVPNPSNVVGQVTGPSNTPPRIVGASINFRPDS
                     TQAPSNLLYLATGSDVRLSNLASLWMREACAAVSACATACPTFTPTCIGARSDMQSGS
                     SWTYSTYANKVYEVNGNNVFFTAVAGKWSYYVSAPGYNNSASSTITLNGQEVNATPVV
                     LTPSTHRAQIAGQSVVLDTLSNGTRNAYGAAVPGFNTQSGVPGMFAVMLGNSDNSGNP
                     VAHITVTNASGAYSFDGNSKVVNLPDITVLCSNSALVSSLYPGVTSLSGASCTGVADN
                     LRVAYAISQYASAQTLSTLSATPNNGLVANDVTSSICSGAACPGTGYQFRGGSYNIIV
                     TDPLKHMTSSSTGAIVNSGTVALGGTLAVVSQVPHLPRMQVTGTITDAISTGALSGAT
                     IELGMDTDSDPNTITWGPVRQDPATLSGSRLSASDIQVPAVTTDANGNYTISNIDPGT
                     YVIRVTRPGYVTQFVQVTVPNSGPAVVANAQIVLDGPRGNLSGRIVIAGGAPFTGTYT
                     LEIVNPNTGTRPTSPVAPASLSSGTSAFTNVPNYSVFSINPGTWKVKFASANYVPVEG
                     IVTIQPNATTNFDIVTMIPGAQAPASISGRLLNALNNQAISTPLTVRIRPGIGVTSGP
                     YALDANNQTIGAITSAADGSYVIPNVPAGNYTIEITGPGFVTTYETVISAGANSANQN
                     ILVSPSLAADEVRIVLSWNATPKDVDSHLEYGDGSTTRKNQVVWNDKCRNPGVNNAVI
                     KSPAQCSTEDLTLDIDVTTGYGPETVTMKGTVWTSPVVTRLGYSLFNWSNEASLSVSG
                     STVKVYKSTGLVRTYNVGPAQVSRWWQMFCFDKATKNITDVGQPGCNASDFFNAPQN"
     gene            complement(39809..40456)
                     /locus_tag="PLF66_05780"
     CDS             complement(39809..40456)
                     /locus_tag="PLF66_05780"
                     /inference="COORDINATES: protein motif:HMM:NF013742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HMZ63585.1"
                     /translation="MKKFESIYIDGFESFINDRLIDRPFPIMKEMEVFAEKNRIPILS
                     PAAGSIVKFLCETYKTSKILELGTGLGYSTAWMLSSGNTLEIDTIDRNAKELQSAHMF
                     LDQIKTHTQKINFIHTHCIEYLKKIEQLSYDFIFIDCDKICYPEIFAILIEKSDQTTK
                     LLFDNVLWHGRLDGSIYTKPSDRAMQEFWHLIEKRPCSKTLFPAGDGLLLVSDFR"
     gene            complement(40453..41280)
                     /locus_tag="PLF66_05785"
     CDS             complement(40453..41280)
                     /locus_tag="PLF66_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004788527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HMZ63586.1"
                     /translation="MYLKFAHANIDFEGISEGGIRTSISIPRYSLLFDIGGIPHEKIH
                     MENVLVTHGHLDHAAGIPYYISQRSLQKLKAPNIYVNEAMYENTKKIIELYSEIEGFS
                     YNYNLISTPTNKEFSLTANTFFKAYQTTHRIPSQGYTIFEKVKKIKSEYASLSSAEIV
                     QLKSKGENLTEEKSLPLVSFSGDTQIEYVLEHKDVRESKILFLECTYVDDDRNVERAR
                     AWGHTHLDEIMAHAKEFKNEKIVLIHFSKRYKHSYIKDMIRKKTPESLKGRIHCFLP"
     gene            complement(41285..42727)
                     /locus_tag="PLF66_05790"
     CDS             complement(41285..42727)
                     /locus_tag="PLF66_05790"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HMZ63587.1"
                     /translation="MKLILNFINFKLKFLFLLFLGISLLAAPGDFVKDIKIDELDFHI
                     PKVSKEDLNPNLKFYSIYSDKFPITYLEIAFYAGEADTEGNGIEIPALLSEVLRFGGS
                     KKYPEETMLSKLESLGAGLSVSSDYDKITINVSYLSRDENTILDILQDLIQNPVFSEQ
                     ALTNGKKKMIEMIARRNERTDSLGFRKAKELFYRGLLAGKVSQATSIENIKMEDLKSF
                     WQLAQSRKKRISVTGLFDSNKIKLSFNEILPAANSNKPINRENVSQEILSKSLKEYKY
                     KNLIITKDVNQSMVLMIGSLPRHNDKDFYALQILDYIIGGGGFNSYMMQQIRVDKGLA
                     YSSSSYPIFKKQHGILYAYTLTKNQSLLQVHDLMKTILSEETFAKIKEKEIEDAKRSI
                     NNQFVFLFVNNNRIVSNQVSFDEDEMPENYLENYQKNINSVTLADILRVGKKYFTFDQ
                     LKTVIVTNKDSADKLPLENVKVILPEDSVE"
     gene            complement(42724..44307)
                     /locus_tag="PLF66_05795"
     CDS             complement(42724..44307)
                     /locus_tag="PLF66_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020768978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HMZ63588.1"
                     /translation="MKSIISVFLGILLLFTQTVSAENIFEDVANRLKKSIKKVEFENG
                     LRLIMMQNNTSPTLALYAKFKVGSVDETKEIAGTAHLLEHMLFKGTENVGTTNYAEEK
                     KYHILLKATGSELDKLRLEKKNFEDQEKSIPKELLERIKRLERRLKDVEEIQKKFIIK
                     SEDTYIYEQHGQVGFNAYTSHDVTNYQIRLPANRLEIWAKMESDRLKNPILREYYTER
                     DVIMEERRMRVENRGIGLLRERFLELAFGEHAYGQPVIGYESNIPFLDIYETENFFKK
                     YYTPDNMVIAIVGDLDFDKTEAMIKKYFSDLKPSSEKKLGTRVREKMNTGEKRISFAY
                     PGGSILVMGWHKPAYPNRDSSVLDLVDAILTQGTSSRLYRRLALKERLVTSVDAYASD
                     PGDRYSNLFSIYTQLNSDADPNKVESIIWEEIEKLKKEPVSKEELQRIKNKMTADFLR
                     DIDNNGSLADNLSYYELLTGTWEDLFLSYDKLNNITEEDIQKVVSKYFVKENLTVGHL
                     DSRNYMAPAKSTQTGKNTK"
     gene            44454..45206
                     /locus_tag="PLF66_05800"
     CDS             44454..45206
                     /locus_tag="PLF66_05800"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HMZ63589.1"
                     /translation="MKLMLNLIIFTFFFFVLNCSSVGDMYVNEAAEGNLSELEAISQK
                     HGDQTNAREERNWTALIAASSTGRVDVVKYLVSKKAKLNLRTNLGDTAIYRASANGHT
                     EIVKYLLEAGANPNIRDKKGDSCLMKAASKGHTEIMRLLMDAKMNPNDVKIPGVDGTT
                     ALMTASLEDQVESIKLLIKYKADLNAKNQNGDTALSLAAGAGKLKAVKTLIEAGANVN
                     NASNFGMTPILHAEENGHRNVVAELQKAGAKE"
     gene            45203..46048
                     /locus_tag="PLF66_05805"
     CDS             45203..46048
                     /locus_tag="PLF66_05805"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HMZ63590.1"
                     /translation="MKWKLWFLFFIFIGSIQAGVNEDLLQGAQEGNFALVKRSIELRA
                     DLDFRNSDGETALILAAWYGSPEIVSYLLESGANINLQDNAGYTAIAKASSLGVGRHY
                     EIVEILIQACANLNIKTKEGKSPFLLALLNGHFELAKVLRNAGAKEEPYFLGAEADKE
                     LLAASSVGDLQRVKYVFFFKPNVNTSNEAGQTPVMLASRVGKKEILILLLKSGALVDT
                     KDYSGKTALMYAARRGNKEAVSVLLDYDADVDIEDKDHFTAATWAERFGQIEIAEYLK
                     RLSDK"
     gene            complement(46121..46879)
                     /locus_tag="PLF66_05810"
     CDS             complement(46121..46879)
                     /locus_tag="PLF66_05810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63591.1"
                     /translation="MQTIVIIDANEEEAKDIDSFLISYKYKTVRIHEVKKGVELTKSV
                     LPDLIVIAIESNDRETMAALQAIRKDPITKDIPIIPTIRSNDRSLLESIRKNGVHDYF
                     VKPVNKVKFIEKIKSLLILAREQKKDNIIFRKHHIVIENPTDTITLISFKSGMKYVLP
                     EIRNIFNPDFLKSLIPKSVSLDIRDLPDLIKDELQIIEKIIALFGNKKISIIAGKHMG
                     FIIASSDLEDKTNLFLSLDDYVAFLKKQATTTPK"
     gene            complement(46985..48934)
                     /locus_tag="PLF66_05815"
     CDS             complement(46985..48934)
                     /locus_tag="PLF66_05815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63592.1"
                     /translation="MKIFIHLRNWNFIILLITIGLSSIIAEEEENILFSIKGKSPIYS
                     KVKYDKYGNRKSISFVRVEENSEHLVELEGLAIAKLKYDEKTKTFKVKEYDIDRNLLF
                     TNKVKILKNGFELITFLGKKKNGKPIFKKITYDIDARKLQEEYLDENKKPNTNKNKIA
                     KIKYKYNQSGKLLTTENFDTQGKYKEDANGIAKYVYTYDARGNKLTSSFFGENEKPKE
                     GKDGYASESFTYDYTTSERGRLVLHETFDGKGQPKENTKGIAKTLHLYDKKGNKIATE
                     FRDALGKLKEDGKGFAKYIFEYAENGNLVLEEQYGSDGKLKLDPADGIARITSESSMI
                     GDTNTKTTRFFDTEGKLKVNEVLEAAIVKVSTKLFKEGKEIRTEYFDTEGKAKLCGEG
                     YAVLIQKYDLFGNQILEEYYDINNKPVLSRDGYARYTASFKEKEMISEEYWNLEGKLT
                     EGDDGFAKQVRTFLPNGKLLSIENFDSNNEYIESNLGYAKMIYSYNADGYKSEEIFLN
                     NKLQPANNSEGIGKITYQYNKSCLSKTKKEEDCLTLVQHFDSKGKPIANKKQIFKIIT
                     NFDEQGRIKSIQSFGSDDSIANRHIYSYELGSKREIAWEIYKKSMVSRAIYNYYILIP
                     ELGSKPVVKIILNKLCRPVGIKLMK"
     gene            49071..50354
                     /locus_tag="PLF66_05820"
     CDS             49071..50354
                     /locus_tag="PLF66_05820"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HMZ63593.1"
                     /translation="MSVQTLPLPLTRQISYAIGQLGWSILINIIGFHQVFFYLPPNGE
                     GLPELIVKVAFFGISTIGVVSAIGRLWDGITDPIIANMSDKWASKWGRRIPFLATGGL
                     PAAVFCLLIFTPPEKGITTGNLVWMTVALLLFYLFLTVYVTPFFALIPELGHTPNERL
                     NLSTFISVTYALGIMVAAQVPLIAEKLMNAVPGMDKFTGYQWAIGILCTIAAICMYFP
                     VLAINEKKYTKSEPSAVPFKEALMMTFKNKDFLFFAISDFNYFLSITILTTGLSYYIT
                     VLLFQEASLVSEILPVMLFSSFLCYPIVNFFAKRLGKKRLVIYGFFIFLLLFIFIYFM
                     GMDSIPIPKVIQAYLIVIVAGAPMAILGILPNAILADIAELDALKTGSRREGLFYAAR
                     TLMQKLGQTIGVLIFSSLLLVGGGINKVEKIDPTT"
     gene            50543..50731
                     /locus_tag="PLF66_05825"
     CDS             50543..50731
                     /locus_tag="PLF66_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002975048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63594.1"
                     /translation="MRYKIEISKLKNGYIEAKLIDVATNHPIEFRICDTEQYVQSQIA
                     DWHKRFRMNEPKEDDIKK"
     gene            50715..51479
                     /locus_tag="PLF66_05830"
     CDS             50715..51479
                     /locus_tag="PLF66_05830"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HMZ63595.1"
                     /translation="MTLRNNLFFILFFFLIHCSSFGKVKNDYFDYYDSSFKCLAETGW
                     RDSNLYSRYLDLGWNPIRKKYNDDNQKIKSANVVIVGNSLIQLFDERLIQREFPSLNV
                     VGRGIAGDMSESLRQRISDNVLSLNPKTVIIEIGGNDIIFGKCLSYTENNIRGIIEDI
                     QSYDKRIKIIFLSVPPTKNKVLNSVVPALNASIQSLAKEYNFVYLDLWKAMRNPDLPV
                     IKEDFVRYNEFGKMDKIHFNEKGYEVIGQLVRPLIK"
     gene            51479..52342
                     /gene="serB"
                     /locus_tag="PLF66_05835"
     CDS             51479..52342
                     /gene="serB"
                     /locus_tag="PLF66_05835"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HMZ63596.1"
                     /translation="MLIFISPSGNPISIKNTVIKRFGEAKYLYSSSLHNIFAHYLDFP
                     NKIERDDLIALRESLSSERIDVLWVEELLDRSSKSLFAFDMDSTLIKEEVIDELARRK
                     GVYDLVASVTKEAMEGKLDFDSALRKRCSYLVGIGESVFEEIYQSITPNDGVVELIPW
                     IQKYKGIVAIFSGGFVPVIEKFAKKYSVLHYSANQLEMENGFTTGKVLGTIVNKNRKK
                     ELLIEHRDNNQILNSQTVAVGDGSNDSLMLNEAGIGIGFHAKSGLKKDILNWIDHHSM
                     LSLLFLYDLRD"
     gene            52459..53997
                     /locus_tag="PLF66_05840"
     CDS             52459..53997
                     /locus_tag="PLF66_05840"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HMZ63597.1"
                     /translation="MEKPDLAPEVIPVPEVPLQFDEKDYKSRKVSKKKPHFTFAPILF
                     PAGFNPELIAVFHQENRLLWQNTESKEVKISIDSWKSQDTLRKEFVEQDIDSFVNTDL
                     AFDGDVLKVKQTFIDPVNQKEYGTIDFEIAVILPGHPDSKSKIELYKTENKFKALEEI
                     KSPILKFIRKPDRNLQITLLRSTVSAHINVSSSSSDTVFYLDGKEMGKLPIRRLLIPD
                     GPHQVTYRKTGLSPVNKKILVRAGEEMNLFQEWEDDLSVGSLKIYSYPKGLKVAWNKE
                     VKGETPFFLYGINPGKYRMEFTKIHDTKKINIPLRDFEVEVESKNTAALVFPMELYDS
                     FLPSAIDLWQPVLGGVNVNYAGNLSLENRSGQVIDKPKGVVTQPILPDSMEISSAFFL
                     PSEMGSGNISFSLITKNATYTLEAEREKIFLYKFPSNGVSIGAYTYDPQIKDQHRAFR
                     YVTDMDKGTLQIYLSDTKVFDDSIDFSDTWKISVLLRGNASPKVNALKYLWIQCPKYI
                     QLLK"
     gene            54018..>55593
                     /locus_tag="PLF66_05845"
     CDS             54018..>55593
                     /locus_tag="PLF66_05845"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HMZ63598.1"
                     /translation="MANLFGFLFLILFVCSQFQFCISIEESPSTKAVEITEKEKKEIK
                     ARNLFHEGNDLYKASSFKEAIQKLEEANSILPTEDNFYLLGLCYLKLDDYKNSKESFQ
                     QALEINPKNERVLLAESLLETGQGKEEDSLEILTRLTEFYPEKDIYRFKRAVSLKSLK
                     RYKESLDEFKKINEKEFSQKSQLFTHLGELYLLLKDNSLSEKYFSKAKALNPDLKDID
                     ASKNKLRYDDLIEKGNLYYYKKNYKEAAHYFEEAVFISASASLYFKLGTCYSEWKNFK
                     KGEEYFKKSLSLEKAKSTFVHLANLYLKENLIFESVNILQSATLNYPEDSELFFKLGS
                     AYKKLANPKLAIISFIRAKDLDPNSILTRKNLAFTYLEESQLVEASREFKDLQALSPN
                     ESEFKDILNILDVTDGKEDVLEKIKKWKNKRTLSQGRNLEDLKQFDKAEKYYLSKLSD
                     SILRDAAKYRLSLLYSIISEEELSRNRFTKAEIFFNKAKEYDSKSIQIKKIESILRDS
                     KKREIANKSYAKAEKLS"
BASE COUNT        17391 a         8867 c        10383 g        18952 t
ORIGIN      
        1 cccatatgcc tcagttgtaa taatgaaatc tacatttcgc tttccggata gatctttggg
       61 aggttcatga tattttaagt tttagtccag agtcatgttt gatggctatt taatatcaat
      121 tttactgtca gaatcatttt tatctaagat ttcttcttcc tctctacctg ttgcgatcca
      181 tttttctctg tgtttttctt cgtgtgcccg aatgatccct ttaaactcag aaccagttgc
      241 ctttgcaatc acagctccaa tcaaaccttc tggggctcca taggttccga ttaacgcatc
      301 ataatttcct tttccaagac aaatatccat gatacaggta agtgaaccgg attcaatcag
      361 agatatgcca catcctagag agcggatact ttcgattagc tccttgtgtc ttggtctatc
      421 ttgcactaca ataattaaat ctgaaatatt tttatgaagt gagtcagaca atttttctaa
      481 attcgctttt atattttctt ttggttcaaa tggatacttc atttgtggtg gagcaaccca
      541 ttgttccata taagtccccg gaactcgatg gagtccatct ctttctgtaa atgcaatcac
      601 acacatagag tttggtaaac cctttgcaaa atttgttgtg cattctagtg gatccactgc
      661 aatatcaatt tgaaactctg aaccacctag tatttcatct tcgtaaagca ttggtgcttc
      721 atctttttct ccctctccaa taatcactct ggactgaaag ggcaattgac cgagaagaaa
      781 tctcatttct tccactgcga tatgatctgc tttttttttg tccatttttc caaatagggg
      841 gaatgttttt tgagctgttt tttcggttat attagccagt gtatttaaaa tatattctgc
      901 attcaggttc atgattttat ttaagtgaat tgaatctaat tcatcacagt ttttgatttt
      961 caactaatct ttttatactc ccttcattat gaaaatcttt ttattccgta ttgcaaaaga
     1021 aaaagtgaca aagaaaacct atgttaaata actgatatta aagaacggaa tgtgtaatta
     1081 ggttttttac cacggataaa cacggatgaa cactgataaa aatgagatat tttaaaatca
     1141 ttcttgtcca acggtgggtg ataaaccaat cgatcgggtt gaagataatt taatactgaa
     1201 attttaaaaa gaaggaatct atgataacaa agattgcgcc ggaatatgag tcatctaagg
     1261 aagtaatgga tctatggaaa ggatttagaa aagaggtcat tgattgcttg cgagatgtcc
     1321 ctacagatga gttgattaaa agtcctgagg gtatatggtc tctttcagaa gtcgcagagc
     1381 atctttatat cactcaatgg aatattgcaa gatctgtccc gattgtcatg tcgggtcgag
     1441 gcaattcagt gtttaacggt tacacaaaag aacctgatta tcgaatgatt cgacttagtc
     1501 tttccaaacc atctggtgtc aaaaatccag actcggttgc tcctctaaat aaatattctt
     1561 ttgcggaatt actacctctt ctcaacaaag cagaatctaa attagaagat tccacaaaca
     1621 aatattcaaa agcagatcta atgaaatata aaatggaaca tccctatttt ggagacttac
     1681 atttatttga ttttttatgg gtaatgtctt ggcatgagca ctctcatcta tatgctatca
     1741 aaaaaagaac taaggagctg agaggctaat cgtgttaatc tggaagattt tttgggaata
     1801 tccactaacg gcttcaatga ctttattttt acctttattt tattttgtca ctcttacctc
     1861 tgtccctgaa cccactctaa gggcttattt ttattcttac ccaggaaagt taagtttggt
     1921 aaattggatt ttagcatctt gttttcattc ttccctatcg catttaatct ctaacatggg
     1981 ttttctttat tttttaggta gagctgtaga ggcaaaagta gggaagggaa aatggctttt
     2041 attttatgga atggcagcaa tcatttctat gatggctgat tcgattgtaa gaggatatat
     2101 cattgtagat agaaatatcc caacggttgg tgcaagtggc gccatatctg gattagccgc
     2161 tattgccgca cttctttctc cagtcaccta tcgtgtaaat ggagttagtt ttccctttcc
     2221 tgtttttttg gttgcctgga ttatggttta tacagacatc atgagagcat tctcttctga
     2281 tttgatagct cattgggcac atctagcggg atttttctct gtctttatta cgacctattt
     2341 gctgagtcct gcagatcagg caaagattcg aaatggtttt ctttttaatt ttactttttt
     2401 tatcttaaca gttatactca tatatttatt agagaatagg tgatacaatg cacataaaat
     2461 tcaattcact cgaatataca gctgacgacc aatttataga atcggaatat gaatacacgg
     2521 gagataaatc ttctggttgg gaaattaaac gaaatggaaa ggcttatctc aagctaggac
     2581 aggggtatga attgctcaag acaaaacttt gtggtgtttg ctccactgat atcgctagac
     2641 gtttcctgcc atttccactt ccacagatta ctggtcatga attagtggca gaaactttag
     2701 atggaaaaga tcattatgta gtagaaatca atgacactcc tgcggctcgt ggagatgcag
     2761 tgcaagatga attttgtata gctggaattc caactcacag tccaactaga cttgttttgg
     2821 gaattgatcg actcccaggt ggatttggac cttatattct tgctcctcag aatgcgatag
     2881 ttccttataa aaagatctct gacaaaacag ctgttttaat tgagcctttt gcagctggtc
     2941 ttcaagcagt gattgcgtca cctcctaaga aaggggatag agtagcagtt cttggaccta
     3001 gaagattagg tagtttactg attgcagcac ttaaagccta taaagaaagt tatcaaattg
     3061 atttttctat tactgcgctt gctagacatg aagagctttt aattttagct aaaaaactag
     3121 gagcagatca gggtataaat ctaaaagaag tctctttaga atctctctat aaatcatttg
     3181 atattgtgta tgatacgact agcacagaga gtgggttttt atctgctctt cgttttgcta
     3241 aaagggaagt ccaccttaag tcaactaacg gtcttgaaat gtgtggtttt aaaaaattaa
     3301 cagaacttgt agtagatgaa ctctctatct taccttttag caatgaaaat ctcaatttca
     3361 aatgggaaaa agaagatcga aagaatgaaa gaatttattc aataagtcca atctcgcttg
     3421 attctaaaaa atttaaattg tatcaaacaa atccgaaaca agcagaagag attctagcat
     3481 cagaagaatt tgctcacttg gttccaagat ttgatctagg aattgcaact tctaaagaag
     3541 aaattgattt gatgattcgt cccaattctt ctcatgaaaa ctctctgatt cgacctcgag
     3601 gtgctatttt attcaaaggg gaaaatcaaa ataatccact tcttagtttt gtaaacgaag
     3661 gaggagccat tcgaacgtct cgttgtggag atttccattt ggcgattcga cttttagaag
     3721 aaaatcaagc gttagcagaa agtctttctg agaatatgat tagccatgtt tacagtgcag
     3781 ataaaatgaa tgaagctttt gtaaaggcga aagattctag tgctattaaa gtgatagttg
     3841 agcatctatg agaactatcg atacatcttt aggcttccca ctgattgaag atgataaatg
     3901 ttggaaaata catttagaag aactcaggct ttttaccggg ctttctgttc tagctcgtat
     3961 tctaggcgat caaattctag atcacatgga aactcagttt agtgatatat ctttttcgta
     4021 taagataaat ccaaatatca atcctgaact tcttcaaatg cagattgtag ccattcaggt
     4081 ctatgcgaag tcgggtgagt tagatgacat tatttttcat atagatgaat ttcattccca
     4141 cttaagaact atattcggaa cgtttcaaag accaaaatgg gcaaagagga ttcatcccga
     4201 attttatgga gaagatccga gagaagtggt atcttctccc ttgctttttc ccttggaact
     4261 tccttcttct aaaaagaatt tactttttgt tttagaaaga gtggctaata gtcgttatac
     4321 gggtaagttt tttatgcgtc tttccattga aactttttct gatgtattat ctattccgcc
     4381 aggaaaagga acacccgttg atgatttgga tgcaagggtt tatattgcag gaactactaa
     4441 actttctgaa tctatcagcg ataaaatctt aacagcaagc aataagggtt tagttgctta
     4501 ttcagaagaa aatactcctt atagttcttt atttgaacag cttcataaaa ctcatttgga
     4561 aaaagtagac cagattaatt tttactggaa tagagatttt tctgaattag ttcaggatat
     4621 tacaaaagaa gaccaagttt taatttttaa aaaaatcttt ttaagtcttg aggatgaatc
     4681 agtttgtaga cttttacaag aaggagaaac cattcgtttg gagttaggac tgacgtttgt
     4741 gaatctatat cttacaagac taagtcgtgt tttaaatttt agtttgggtt tagagaaaaa
     4801 agatttctca cttgattatt atttaaaacg aatgccactt ctagaaaaat taacagatac
     4861 aagtcaacac ggaaaagatt tttcaaatat tcgtatcttt ttaattcacc atataacttc
     4921 agaaatcatt gcagtgattg atgcacttcg taaactgaaa gtagatgcgt tagatgttat
     4981 atttgttaaa tacgctggag tgattccttc taagtattta gatgttctat tggatatttc
     5041 agataagaat ttttttatgg caggtttaac tcgtcatctc acagaaacca acaaagaata
     5101 tttcacacca gctaaatatt atacagacat tagttctcat gtaaatttgc agaaaacatt
     5161 gcaagaaaag aaacttggat tctttgaagc tatgaagtat ctttcttgct atttcttttt
     5221 gttattttta aataaatcga ttcaagaggg gaaaaaggtt ttaatcatag aagatggcgg
     5281 atatctttct cctcttttaa atgaatatgc tctatcggaa aaattgacaa aagatgttta
     5341 taattcctat caaattacag acattcccaa agtagaatca tttagagatc ttctgaaaga
     5401 gaatttagtc ggaagtgtag agcatacaag aaacggttat gataaattag catttgtaaa
     5461 agaaaaattt gggaagttaa atcatattgc ttattcgatt gctatttcta aaaacaaagt
     5521 agtggaagag tcaaaagaag tagctcattc tatcttgagt gctatcgaat caattttaca
     5581 tggacagggc atggttttat cctctaaaaa aaccataatt ttgggagcta tgggaaatat
     5641 cggtagtttt ctcactaaat atatgaaaga aggaagacta catgatacca acaaagaact
     5701 catcggggta gacattcgtt atccgcaagt aacaaaggaa aattataaag ctcttcagga
     5761 aattccggat gaaatatttc tatcactaga attatttatt ggagtaatcg gtgcttcgat
     5821 ttttaaaaga gaatttcttt ctaaaatcat tttacaagga acgaaagaaa ggctaatctt
     5881 tgcttcaggt tctacaaaaa cggttgaatt tacggactta tctgaatacc tatatgaaat
     5941 ttctaccatg gaatctcctt ctattgatgg aattccagtt cgaattgtat ttgataaaat
     6001 catcgatcca caaacggata cagtccaagg aacaaaagta tttatttcat ttcaaaaaaa
     6061 ttctttggaa attacaaaga cattgtattt attgggagac ttaagtccta taaattttct
     6121 attttatgga gttcctacag aaactatgga tagaatcatt tcgcagctaa caagcgttag
     6181 tctaggtatg gtagaccaat ataaaaaggg aattcttcct gaaccaaatc tatatgcagt
     6241 ggatcatcaa attgatgaat ggggaaatac attatgagta aacatgggtt gtttcaaatt
     6301 acccaaaagg tttttattag aaaggacgat ttgcttctcg ttatgaaaga taaaaagtca
     6361 ggagcgggag atcttcctgg tggacgaatg aatcaagatg agttttttgg agactggatg
     6421 gattctctta aaagggaact agaggaggaa cttggttctg agtttcaggt cgacatttct
     6481 ccaaagccaa tcttagttca taaacattta gtaaatgatg gaaatcatcc ttgtgtgata
     6541 gtcggatact cgggagtcta tcgttctggt gagatatcta tttcagatga acatgatttt
     6601 tttaagtggg tagatgttaa aacctatcaa ccagatgagt tgttttctga gtatatgtta
     6661 gatgctgtta aattatatct aaaggaattt gcatgaatcg tttgtttcgt tatattttat
     6721 ttttttcatt tgcgattgtt agtttttatt ttgcttggtc acaaggagga agacagataa
     6781 atgaatctga atatgagagc ctatcctctt ccattcctcc actaattatt agaagtttag
     6841 gatatgatcg gaacaacgga aacattgtag gaattcaacc tattataaat ccaatggatt
     6901 attcaagcga agaaagattt tatgataaaa tagactctta tcttcatact gcaaagaaaa
     6961 gaggtttttt aaatgagaat acgatagttg cttttccgga acacatcgga actggacttg
     7021 ttctgctagg cgaaaaatca tcagtatact cttctagtag tttaacaatt gcttttaccg
     7081 aaatggcatt tagcaatcta ctttcttata ttggcacttc ttttactaaa acgcatgact
     7141 ctcaaaccga acttgttttc aaaatgaaat cagaggagat gagaaatagt tatgtagaaa
     7201 cttttaagcg actttcctct gtctataacg ttacaattct tgcaggatct attcttctac
     7261 ctgaaccaga aattgataaa acaaagaaca caattcaatt aaaaaatgga aaattacaaa
     7321 atgcgagttt tattttttta ccggacggaa agatctatga tcaaattgga attaaaaagc
     7381 aacttgcggt atcagaaggt caaatctcta ttccccgtaa agcggaaatg ggtaatctaa
     7441 aatataagaa tatattaatt ttattttcaa atgattcgct ttataattcc atctatggcg
     7501 aaactggaaa ggatacagag atagtgttag taattgccgc gatcttacaa ggagatagcc
     7561 tagactggaa attagaggat atcaacggag caaaagaacc tgatcttggt ttgactcagg
     7621 cggagctttg gagaaaatac aatgtttttg ataaaacgag aaattctctc agtaataaaa
     7681 tcactatgca aattgtattg cttggaaaat tctttgatat gattttagag ggagaaacgt
     7741 ctgctttgat tcgctatgtt gcaactgagg gtatcgacaa taaaagtcct gcaatcctaa
     7801 atgtttggtt ttagatgctt gacaatttta aaaagaaaaa ttttaattta aacatgactg
     7861 aaataatttc ccagaaagaa ttaaacatcc actgtcctta ttatctaaaa aatattaaat
     7921 cctgtccaag tgctaagaaa atattatttc ccgaggatat ggaaaggttg gaaggatgtt
     7981 gtttatctga gacctattcc tcctgttcaa tttatagaga attaattgag aaggcagctt
     8041 aattttattc tgtcatttct cagattaggg ttttgatcca atctaaagaa ttttctactg
     8101 tttttcaaaa agctatttag gtgattagaa aacccaaata gccaaagatc cattctaaat
     8161 cacaagattt tcaatctgta catactattt gtttttctgt ttcacttgtt tatatagttc
     8221 taataacttc cattcctctg gagaaaactc tgtgctagta agtatcagtt cttgcgaatt
     8281 gttttcataa agtataaaat ctcttgctac tacccagaat ttttctgcta agatcggata
     8341 caatttttta gaaacatttg gtccccatct gtagatgcta cctgactttc ctgaaataaa
     8401 ttcgttcgct ttcgtagttg gaaagggatt gatttcattg taagttattg gaaagccttt
     8461 tcctatgttt ggctctccat aggctgtgta tttgatttca cgaagtgtga gagatcctac
     8521 aacgcccgat aagacaaagg catttttccc atttccgtaa taaagctttg gtgtaacaat
     8581 ttctgtggct aattttgtta tgctgttgtc ttttgggttg aaccaatata gattatagac
     8641 acccgcattt ccaaaaaaga ttagaaaggt ttttgcaatg gaaaaaatat gagttctatt
     8701 tttaatattt ggataggttg aatcaaattt ttcctttggg actagtaatt taggtttttt
     8761 gggatccttt aaactttctt catataaatt ttcattgatg aaataataaa tactagaacc
     8821 gtcagatgat atagcagttc tatttttaca tgaaacatat gtatctgata aataagaagg
     8881 ctttggttca attagagaaa cattgaccat acgacatcca gctccatttg ggagtttaaa
     8941 tagaacaatc gcatactcag cctccgagga tacactgacc atctcaggga catctggaaa
     9001 agttaaactt ttactcaggg ggttcgctaa atctctaaat gtaatttgct tgtcttctgt
     9061 ccatacgatt cgatttctat cattggacat ttcgataatt ctaggattaa taggtttttt
     9121 ttgcgatagt atttcgtctg acgctctttt ggtttcaagt ttatttttaa ttttatcttg
     9181 cgcctgtgaa aatttctcat cttcaatgag agtatctatg tctttgacgg cagattcatt
     9241 tttcccatcg cagttgagta gaattgcaag aaggattaag ggtaaatact tcattcgctt
     9301 tgttttaatt caccggattc tattaaaata tcttttgcga agagtcctat ggcagttgct
     9361 gctacatact ttgtatctaa atacgttaac cctcccgtga gtgctgcacc aagaagtgga
     9421 agccatttta aaagttgtga tcgaatcatt cttttagaaa gattcattcc aattttttgt
     9481 aaaagagatt ctaagaatcg aatggtggtt ggacgaatga taattttagt tccaacatct
     9541 tctaaaagtc tttcaaccaa atgtagactt gtttgtttga acatgcaata gattaagagt
     9601 tcctttgtca caagagattc ttttccatag agagaggcaa tgtctttgat gagatgaccc
     9661 tgaattctat agatcataaa gagttcaggc aagattgtta tgatactaaa aagtcctgtt
     9721 ggtaaggaaa gtcctgcact gattgctgcg gttttgaagg cagctgcctg tgttaattcc
     9781 tctgcaattt ctgctggatc tcctattgtc tccgcataag gactagtata ggcttctact
     9841 ccagtgatca attcaaatgc tctttcggta aatttgtcct gtgccattga aatcactttc
     9901 gttcgagtgg aaattttgta aattagattc tcaacttgcg cactcgttat cactccggcg
     9961 aaacagcaga ggaatttaag gattaccacc gataaagacg atggacaccg ataattgaat
    10021 gataaaaatt tatcgattat caggaggatc gttttctgaa tcaatgatag caggtaagca
    10081 agcactaggt tgtagtcttt ctttcgaact gggtagttat taaaatttag cttttcctaa
    10141 gatggctttc aaaaaacatg tagagacagt tgagacattc gtcatgaaag atagattatt
    10201 aaaaattcag cagaaattca taaaaataga tgaagaactt tctagtccta atttaaattc
    10261 acagaaatta aaagaacttt ctaaagagcg ttctcgttta actccaattt ataacaagat
    10321 agaagagtat tttaaaacag agaaaaattt agaagatgca aaagcgctta ttgaaagtga
    10381 aacagatagt gaaatgattg gaatgctcaa agcagagttg gaagagtcta actccttgtt
    10441 atccgctcta acacaagaac tggaagtaat gcttcttcct ccggatccaa attctggtaa
    10501 aaatatttta gtagaaattc gagccggaac aggtggagaa gaggctgcct tatttgctgc
    10561 tgatcttttt agaatgtata ctaagtatgc agaaaagaaa cgaatgaaat atgacattat
    10621 cgatatgact ccaaccggac ttggtggata taaagaaatc attttttcta ttgaagatga
    10681 gagagcttac gatctattta aatttgaacc aggagcacat agagttcaaa gaattccaac
    10741 tacagaagcg ggaggaagaa ttcatacaag tgctgttacc gttgctgttt tacctgaagc
    10801 agaagaagca gaaattaaaa tccaagaaaa tgaaattaga gtagatgtgt ttagatcttc
    10861 tggtcccggt ggacagtctg tgaacacaac ggactctgct gttcgtatca ctcatattcc
    10921 aacgggtatt gtcgttagtt gtcaggatga aaagtcccag ctaaaaaaca aagataaagc
    10981 tatgcgtatt cttcgtgctc gtattttaga gaaacaagaa gaagaagcga aggcaagttc
    11041 agatgcactt aaaaaacaaa tgattggttc aggagatcgc tccgagagaa tcagaaccta
    11101 taactttccg caagagcgtc taacggatca ccgtattaat tatacaagcc acaacctatc
    11161 tgctatttta aatggggata tggaagattt ggtaaatgaa ttgatccaag ccgatagaat
    11221 ccaaagatta caagaaagta aaacagttta atttgagttc gatctaagat tttttcacca
    11281 ccgatgcaca ccgataactg aacgatgaag acacgatgga taattttgaa ttatcgtcac
    11341 ttttatcggt gataatcgtc cctatcggtg gtgaactttt aaatctttag ttagatcgaa
    11401 ctgacgttaa attaaaactt cttgtttgga gttttaaata caaaaaaagc atcgtttccg
    11461 atgcttttct tgattcatag atttgtagga agttttttat ctttttggta atacaattat
    11521 atccacacgg cgattggctg cgcgattgga ttcagaagta tttggtttta ctggattgct
    11581 atcagcaaac cctccagcta agaattgagt cttctgtaat tgacctttct ttagatattt
    11641 caaaacagta agagctcttt caaacgatag atgccagttg tctctcattt tactattggt
    11701 tggaactggt ctattgtctg tatttccttc tactagaatt ttttcagtag aatgtttctt
    11761 taaaactttg attatattat ctagagttct ttttccttct ggagtaagct ctctagatcc
    11821 agtttggaag tttacagaac ttgcgaggct aatgattgtt ttcccgcctt ttgatgaaac
    11881 ttgcgcagat gctggagttg tgttagaacc tgaatttgat ttggatttgt ttcctttttt
    11941 gatttctttt tcaagtgctt cttctaactt ctgagcttgt tctgtaagtc tttcaatttc
    12001 ttctttgtgt ttagaacctt cttcttctag ttgttctttg gaagtagcta gatcagattc
    12061 caaagtttct ttatcttctt tgagttctgc aatttctttg tttaaagctt caattttgga
    12121 ttcgtattct tttttcatat cctccattgc tgcatttttc ttttctagct ctttattgtt
    12181 tgaagcaatc gaagtagtgt ttgcattttt ctcttggttg attttttcaa tctctgcatt
    12241 ggcagctttt aatttctcag ctttttcagc ttcggttaaa gttttatctg cttggatttt
    12301 ttcaatacga gagttgactt ccgctaaacg tgtttcgatt gttttgtctg ctttagatgc
    12361 ttgtaagtct ctttttaatt gatctctttc tttggtaaga gctgccactt cattttcaag
    12421 ctgtgcaatt cttgtgattc cagtggtaga ggttgtttcg gttgttgtag ttgtagtggt
    12481 agtagaagat gatttggcat ctgctacctt agcatcagct gttttcgcat cagcggattt
    12541 agcatctaca gattttgcgt ctttactgtc agctagtttt gcctcggatc cagctggtga
    12601 ttttgcagtg ctagtagatg tttccgtggt tgtagtagtg gtcgtagttg tttcttttgt
    12661 ttgcggattt gcaggactga gtggattcgt cttagagtta tacatattat tgtaatttgt
    12721 atctaactct ttttgtaatt tatctctttg ttttgtcatc tctgctaaat ctgcttcaag
    12781 ctttttatta cgagccattg catcgtctaa cgactgattg gatgaaataa cagttggtgt
    12841 agatgtgcta gttgtagtat ttgctgttgt tccagcagca gttttggaat tattttctat
    12901 aactgtcgtt ggatttgcag gttgaccaac ggttggagtt tgatatccat agattgccgc
    12961 aatatctctt tgtaactggt ctctttggtt tgttaaacta ttcacttgtg cttgcaagtc
    13021 tgctattcgt ttgtcttggt ctgttgtaga tgctactgga gctagtgttg gagtagtagt
    13081 tgctgctgct gcggcggcgg atttagaagc agaggtagtt gtagtagtag aagactctgt
    13141 gcttgtagta acagttgttt ttgttgtttc ttttggagcg gcaggagcat agttacttgg
    13201 aactgttgct ggagtttctg gtcttacagc ttgcaattga cctgcttttg catctgctaa
    13261 atctttttga agtctatctc tttcctggat tacatcaata agtctagctt ccaaactttt
    13321 tacttgcgct tgagaatcta gtaataaaga ttctgtgcta gctccacttg gggttacgct
    13381 gttaataacg tatccttgtg aggaagtaga ggtagaagtt gtagtagtcg ttgttgtatt
    13441 ttgatttttc tttagcgagt cgatatacgc attgtttgcc gctaaatctt tttgaagttg
    13501 gtctctttgc gctgtaaggc tattgacttg tgcttgtagg tcagagttat tttgattgga
    13561 tgctacagtt gcattttttt ctgcattagc cgctgcaatc gatctttcta atgttttaat
    13621 tctttcctgt gcctgctgtt ctgcaaatgt ttttgcagct aagtctcttt ctaattgttc
    13681 tttttgtctt ttggtttcag caagttcttg ttgtaaggat gcaattctct gatctgcatt
    13741 ttgagtttgt gtatcacgag atgcgactct gttttgtttg tctgcttcga gttctgcatt
    13801 tagttttttg aggcgctcat tttctgcttt cagtgcctct gtatcctttg tatatttcgt
    13861 ttgcaattcg gtatacttgg catcgagagc tttcttttgt tccattaaat ctgttacttc
    13921 agacttccaa taaaaaagaa ttgcttcttt ttgttttggg tctgctggag ttttttgtgg
    13981 gctagtttct tgagcgatca tgagatcatg gcttagagca atcatgagcc cgtaacctag
    14041 atagcgaatg tacttttgat tcattgtatt acctttaaat cctgagaatt cttttagagt
    14101 aaaaaaaaat cgaaattgaa gtcaagaaag aagctaaaga agatgcaaat ttttttcatt
    14161 tcctctttac atcttgcttg aatagaataa attgcatgaa aggattgaat atttttgaat
    14221 gaacaagcaa tcggatttac ttaacatcaa tttgagtttt attttactat taattcttct
    14281 aactacctat cctatctatt ctgatgaatg catcagtggg aattgtcaaa atggtcttag
    14341 cacaatgttt ttttccaacg gggataaata cacaggcaat tttaaaaatg gcaaacaaaa
    14401 tggaaaaggc atgatgactt ttgctcctcg cacggatgca tctggtaatt ttatttatgc
    14461 ttctaagtat tcaggtgaat ttaaagaggg tcgtttccac ggaaaaggaa tgatgtattt
    14521 ttacaatggc agtcaatacg aaggagaatt taaagacgat cgattcaatg gatatggaac
    14581 ttatgtattc ccaaatggaa ataaatatga aggtgagttt agagatgatt taaagcatgg
    14641 caaaggtact ttaacctatg ctggtggtgg aaagtatgtg ggcgagttta aatatgataa
    14701 gttcgatggg aagggaaatt ttgtttatcc aaatggcgat gtgtatgacg gcgaatttca
    14761 aaacgagtta aaacacggtc gaggaacata tacttacacg gatggaagaa tctattcagg
    14821 cggcttcaaa gaagataaac gacatggtca aggaacttat atctacggtg gtggaattaa
    14881 gtatacagga gaatttgtag aagataaatt tcaaggtaag ggcacaatag tttatacaaa
    14941 tggagttact tatacaggtg aattcaaaga ttataaattt catggaaagg gctctatcac
    15001 ttatccaact ggttctaaat acactggtga ctttcgagat ggaaaaccag aaggggaagg
    15061 aacttatatt ttcaatactg gtaaaaaata cgtaggtgga tttagggatg gtaagtttga
    15121 aggggaggga gttttacgct atgccaatgg agatgaatac attggtggat ttaaaaatgg
    15181 aaaatttgaa gggcagggga aaattacgaa aaaaaatgga gagacctttg tagtagaatt
    15241 taaagacgga ctcttcaatg ggcaaggaac aatgaaatat gccaatggag accgctatgt
    15301 tggagaattc aaagatggaa agaaaaatgg gcaaggcact atgaaattta aagatggggc
    15361 agtctacgaa ggtgggttta gaaatgataa atcccatgga aaaggtgttt tacaatttgc
    15421 cgaaggtgat aaatatgtag gcgactttga aaacggaaat tttaacggta tcggcactat
    15481 gtatgataaa gacggaaata taatttacac tggaaaatgg atcaatggag atccagaagg
    15541 aggaatgaag tgaagaaaat ttttttactg agttcaattt tcgctctaat ttcaacatct
    15601 atttatgcag gggattgtgt gagcggaaat tgtgtaaatg ggcaaggaat tatgacgttt
    15661 gctaatggag ataaatacat tggtgagttt aaaacagaaa gaaaatctgg gtatggagtc
    15721 tatcatttta gtaatgggga tagatatgaa ggcgatttta aagatgatta tccagatgga
    15781 tacgggaaag cgtatttagc aaatggaaat tattatgaag gcgaattcaa aaataatttc
    15841 ccaaatggaa aagggacaat gcgataccac aatggaaacg tatacattgg ggaatggaag
    15901 caaggactca aagatggttt tggaaccatg acttatgaaa atggaagtaa atatgtaggt
    15961 gagtggagaa acggaaaaaa acatggtcag ggaagttata cgcaagccac tgctacaaat
    16021 ggtggcgaga ttaaaaaacc acagaagact ttggaaacaa gaatgacagg ggaaaagaaa
    16081 tcaaccccat tctggtctgg aacgaaataa taaaacaata ggaggatttg aaattgcgaa
    16141 ccaaattttt tcatttagtt ttatcaatgt ttcttacaac tgggcattta ctatcaagtg
    16201 agtgcgttag tggaaattgt aatgatgggc aaggcactat ggcttattct ccaacagaaa
    16261 aatacattgg agaatggaaa ggcggtcaga aaaacggaaa aggaatctac atttttggca
    16321 atggggataa gtatgatgga gaatggaaga acggaagttt ctttggtcag ggtattttca
    16381 ctttcgcaga tgggggaaaa tacattggtc agtttaaggg aaattccttt cagggatatg
    16441 gcactttcta ttatccagat ggttctaaat atgcaggtca ctattccaat ggaaaacgaa
    16501 atggctttgg aacttataca tatccagatg gagccgaata tgctggtgaa ttcaaagatg
    16561 aaaaatttca tggtcgcggc aaatacaagt atgccgatgg agatgtctat gtcggagaat
    16621 ttaaagacga tagatttcac ggggtaggag ttctcacatt aaaaaatgga gatagatacg
    16681 agggagaatt taaatttggg ctaaaagaag gggcaggaac ctatgtgttt gctaacgcaa
    16741 gtaaatatgt aggagaattt cacaaagaca aataccacgg acaaggaacc tattactatt
    16801 cagatggaga taaatacgaa ggtagtttca aagatgataa atttagtgga gatggaattt
    16861 atacatttgt aaacggaact caatacatcg gaaagtttga caatgataaa tacagcgggc
    16921 agggaaaagt caattatagt gacggaaata aatacacagg ggagttcaaa gacgataaat
    16981 accatggcaa aggaagtcta gtctacgcaa acggagattc ctatgtagga gaattcaaag
    17041 atggggagta ttatggcaaa gcaactctaa cctatgcaaa cggggataaa tatgtgggtg
    17101 agtttgaaaa tggaaggaga aatgggaagg gaacttattt ctatgcaaat ggcgataaat
    17161 atataggtga cttcaaagat gataaatacc aaggcaaagg tctctatcgt tttgcggacg
    17221 gaagccaata taagggagat ttcagagatg acaaatacaa tggatatgga gtatatcttt
    17281 atacatcagg cgataaatac tatggggaat tcatcgatga cctaaggaat ggcaagggaa
    17341 tatatatcct cgcgaatgga acaaaatatg aaggcgaata taaaaacaat aaaaaagatg
    17401 gtatgggagt tctctatgat aggaatggaa aggtaatctt ttctggacaa tgggtcaatg
    17461 gagagcctag cagatagaat tgtttttaga aattatagat atttgaaaag aaagtttagc
    17521 agtacgggtg gtaagaatga ctaagtttaa taatcaagat ataggcaatg ggttaatggt
    17581 aaaaccggaa gatatttata tctatcctgt gagtgagcca atctacaaat tcatcgaaaa
    17641 agtctggtcg tcttttgtaa ataaaatggt cggactggtt tttgcgaatg ataaacacat
    17701 gtttgatcat gcgattttcg aagtgatcga tgggacagaa ttaaagatcg ttgcaacagc
    17761 tactcatttt aaaatgaagg agatgattgg caaaaagtct tcaaaagaca ttgaagagtt
    17821 catgaccaag acaaaaccaa tttctatttt tgatgaagga aacgactccg ctagaatcat
    17881 tcaaaaagca attttagcat ttagtcattc aaaacgtcct gatgtagaaa ttatcaattt
    17941 acgagattcc agaatccatc ctaatttacg cgttttacta acatctacta tgcgttactc
    18001 ggtaggtata cctctcattg taaaagaaaa tccgattgga attctatggg gaattcgaaa
    18061 agagaagcta acagaagccc aaaagcacga gattacaatg caattgcata cgctctttga
    18121 cgtaattgaa tatgtagtag caagggatgc agataataac ggtgatcctt actttgctcg
    18181 aaagaatatt gaaaagtccg atacaacttc ctcaattcat catttacttt atacagttca
    18241 gaaaattcaa aaagatccgg tcacatcgat tatcgcacat tcacataaat acaacgttat
    18301 gtatagactt gatgccagtt atatagttcc gacggcagga ggctattcag ttagtttaaa
    18361 acactttgct cgcgaaaatc taaatgatac agataaaatc atattaatga ttcccggatt
    18421 tttctgtcgt agatctgtca tggataaact agcaagggaa atggcacttc gatatggata
    18481 ccgcgtattt gcgatggata tgcgcggtag atcgaaacat acaattccaa aagatataac
    18541 aagtaaagtt tggaccttag acgactatat tcaagatgat tttcctgctg tattaaattg
    18601 gctctctcat aactatccaa ataaaaagat tgtagtaatg gggcacagta tgggagggat
    18661 gattcctagg ttttatacag gagcctacga aaaattaaaa aaacttcctg gtaaagcgaa
    18721 tttaccaaat ccttatgatc atattgcggg tattgtatct attacatctc ctaattacat
    18781 gaatcttgag tcacagatcc taggattgga tgtgattaag agtggcgtaa agttaatctc
    18841 aaacaaagcg atttatgata cattatttaa tcttgtatct tttccagtgc atacaactgt
    18901 caattctatc gatttaaata aattctttcg atttatatta aattttcatt cctcactcag
    18961 acagttttct ttttctgtga gttctaagat tgtaaatcta aatgacttta ttggatacaa
    19021 gcaaattact ccacctgagt ggtatttctt tgtagaagat gtgttctgtg aagaatcaat
    19081 taaagtattg ttgcagttcc tacggtcaca actcagtcat gagaaaagtt tctggtctta
    19141 tgacggaaat atcaattata cacaagaaca gaatgacaat tttgaattgc caatattttc
    19201 tgtgataggc aatcttgata agattgttcc ggcagagacg attgaggatg ttttaaatct
    19261 tcctaagccg aaaaataaaa aagtggctcg ctatgaccaa ggtcatcttg gaattatttt
    19321 ccatcatgat actgttatgg acatcactaa aaaagcagac gaatggatta aagacttata
    19381 ggagaaaaaa tggctctatt tatcactcat ggcttctttg catccctctt ttctttttta
    19441 tggagagata aaaaaacaaa attttcagta ttgcttttga ttttactggc ttcgatcatt
    19501 ccagatattg attttatatt tgacgataga aattcaggga tgttttacca tagaggaatc
    19561 actcattcca ttttcttctc agcggctata ggtgttttgt tttccgcttt ttttatgtct
    19621 tcttttaagt cttttgggca aactctttta ctgatgttta ttttctttct agcaggtttg
    19681 tctcattctg cgttagattt attgactgat gcgcctaacg gtgtttgcct tttgtgtcca
    19741 tttagcgaag aaaaatattc ttcccctttt actttgtttc attcctatac agccggtatt
    19801 gcaccaaagg ggtttagaaa aggagtcaat agtgcttgga gttctatgat tccagaaatt
    19861 ttctttgtat ggattccaac tttatttgta ttcttttttt ctgcctattt ggtaaacaaa
    19921 actactcctg ttaaaaagca gacaacttac aagccagttc aaattaaacc aaaggaaaca
    19981 aaaaagacaa attatccaaa aaagaaataa ttatttgttc ttctctctgt attcacttgg
    20041 tctgagtcca gttattttaa gaaaatggga attaaaagtt ccctttgaat taaaaccgac
    20101 ttcataggcg attgataaaa ttgatctttt gggatcgctg agtagaattt gttctgcttc
    20161 tttaattcga tatccattta taaactcatt aaaattcttt ttataggtct catttaagat
    20221 gcgagaaagt tgatgcggtg ttaaacttaa ttcttctgcg agagagttga gtctaagttc
    20281 ttcgtctcga tagattttat tttcctccat cagttcatct aacctcttgc aaacaacgtc
    20341 tacgttgact tcagtaagga gggttgtctc atattttttc tgcttaatat cattttgaag
    20401 agaaataaaa aatctaggat acttagtatt aaatacataa attaaaataa tagtgattgt
    20461 taaaatacaa actcccaatt tataaattga gtctatttga tttaaaaatc caatcgagat
    20521 aaataaaact ccagcgattg gaacatttag aataatccat acaaaagaag tgtaattgat
    20581 ctcatattgt ttagtgattc ttaaaaacat atggaccatg tagccgaagt aggtaagaat
    20641 atgaagcata gcaaatatat agagcaattt atagggagtg attgactttg tgaataaacc
    20701 ttgctcaaca attgatattt tttgttctgg atttaataat acaaaagcag tatctaaaaa
    20761 aagagctatc catgctggaa ttaaatgtag tcttagaaga actttcttat cactcgtaaa
    20821 atctgttacc gtcactccaa gttttaaaac cattggacct attgctaaaa tgctcggaac
    20881 gaaactaaag gtaagatttg gatattttag aataagcttt gtagagtaaa aaccgagcct
    20941 gagttgaatc agtcctagaa aaaatccaat aaaaaataag ttcccgattg caatggattt
    21001 ttctttcttt gaaatttcta gtagagcgag taaaaaacaa agtcctgttc caaattgaat
    21061 gaagaaatcg gcgaattcat ggggtaacat ataaattaca aaatgactta gcttatctat
    21121 tgagcaaaca aaaaagaaaa gactgtgttc taaattataa tttgagacaa ttttttttgt
    21181 aagtaattat aattcccgcc gacgaatgaa agaatttaga ctagaataat aaaaacagga
    21241 gattatcaat ggcaaatgat aaaactatta aagtaagaca tgtagatttt ggattacaaa
    21301 aagaattacc aaataattat ttcaaaggga attatttcat tacgcatttt gtgaatagtt
    21361 tccatttgat atttccagat ggagaaaaat attttattcg tgcagttaaa tcacatgaag
    21421 actatgtaaa cgatccagag ttaaaaaaaa gaatcaaagc attcatcgca caggaagtaa
    21481 cacatggaaa tgagcataaa aagttttggg aatcactaga aaagaaagga tatgatataa
    21541 atactttctt aaacttctat aattctagtt cttatggctg gctagaacca aatctagaaa
    21601 aaatatttgg cacaaaacac gcattagccg ttacagttgc tctcgaacat tataccgcaa
    21661 gtattgctga atttgcgttt aataatctaa aagaattaga aggtgttccg gaagacatga
    21721 agaaaatgct tctttggcat gcgtctgagg aaattgaaca taagtctgtc gcatttgatg
    21781 tattgcaaga agttgataac agttatctgc ttcgaatgag agggcttata catgcaacag
    21841 caacacttag cttttatacc ttagtaggat ttgctttttt tatgctccag gaaatgaaaa
    21901 aaggaacttg gatcagtcct attgagtttt gggatttctt agttcatttt ttggatttag
    21961 ctaaaacatt gggaaaagaa atagcaaatt atatgaggcc agattttcat ccagatcagg
    22021 tagagaacaa tcatctagct gtatattttt ttgaaaaata ccatgtcgaa aaagcaaatt
    22081 taaaacttgc ggtctaatta gagaaaacgc attagaatca gattagatgg aagcgttatt
    22141 cggtaggcgt tccgtaaata tcagctagct tgagtgcttt ttcgaatgtt aagggcttta
    22201 ctaaataatc tcttacgata ggaaatttag tagccctatt tttatcaatt tcttcatctg
    22261 agcttgtaaa cataataatg attggaatcg gtagttcaga ttttgcagtc gtaaaacttt
    22321 ggtattcttc taaaaattca aatccattca tagctggcat atttatatcg agcaggatta
    22381 gagaaggagg gaatgcattt ggatgttttt gagtagattc ctcatagttt tgaaataaat
    22441 gcaaagcctc tttcccgttt gctgcggaat agatagattt gaaacacttt gtgctttcta
    22501 ataatttttt tgcaatgaag aactctaaac tgtcatcatc tacaattaat accgagtcta
    22561 caattttttt attcatattt tcagatttgg taagatgact tgaaagatag tatcatttct
    22621 agatgaaaga acctttattg atccttccat tttttcaata tgtttcttaa caacatataa
    22681 acctaaacca tttccattga cttgcaagga atgagcacga aagaacatat caaaaatacg
    22741 ctcactgtat tcagaatcaa atcctattcc attatcggct atcgttacta ttaaattatt
    22801 ttttatatca tatttggaat gtattttaat ccaatgtttt tctttgtttg gatcataaaa
    22861 ttgaatagaa ttttctaata tttttttaat aatttctttt aacctagatg ggtaggttgt
    22921 gattagattt gcttccaatt cgatttctat ttcgattttt ttattatcta attcttgttg
    22981 aaatgaattc tggattttgg aaataatttc ttgtaaatta agactctcag ggttttgatc
    23041 tagagcatcc acttttgata attctaaaat attttgaatt aacatttcta atttctgtat
    23101 tggttgtttg attttatcat gataatcagt taggtttgtc cagtcttggt ctttgatgct
    23161 gattgcggat aattcgagta atcctctaac tgttttgatt ggtgctacta gatcatgaga
    23221 taatctgtaa ttaaattcta atagttcctg attcgctttt tctaagagtc gtttagattc
    23281 taccatttct gtaatatcaa tacaagatcc tgcatatcca gcaaagtttc catcattgtc
    23341 attccaagga tttcctgtat ctagaatcca acgatattct ccatcatgtc ttcttagacg
    23401 atattgcata cgaaaggatt ctcttttatc aaaggctgtt atatagatat ttagacagcg
    23461 atccatatca tcagggtgca caccttctgc ccaaccattt ccatactctt gttcgagggt
    23521 tcttcctgta aatgtaagcc aaggtttatt aaagtaagtg caaagtttat tagtttcagc
    23581 gatccagatc ataacaggtg cattgtctgc catgatttta aaggagtcat cttgctctct
    23641 tggtttcatt aaccctccaa aggtaaagtt tttaatttaa aaataaaggt caagcctatt
    23701 aattgagtaa aaatttcact ataacctaaa tgactatcgt tttcaaattt attgaaatca
    23761 atttgaaggt ttttatagat ttcagcgaac tggtaaattg ctaaatataa atatttgatt
    23821 tagataaacc attttgattt aaattatctg ctttctgagt tataaataaa aacaaaatat
    23881 ttcttagatt caatagataa atagtttaca atttactttt taaggaatat gtttttgctg
    23941 tgcgtttcct tttattcata ctgatcctta tccttaattg taagcctagt ttaaaaagaa
    24001 gtcctcttga atattttgtt ttgcctagaa acaacgtaaa tgcaacagga tcttctattc
    24061 caacagctac tccacttagc acaagtaaag atataactgc ctattcaatt ccatctttgg
    24121 gtattactgg aattatcaat ggaactcaaa ttagtttggc atcagacaca ctcactagtt
    24181 tttccccttt ggtggctcga tttacgacaa cgggaaagac agtaacagtg gcaggaatta
    24241 atcaagttag tgatgtaact tctaattcat atagttctaa tctaacttat gttgttacag
    24301 cagaagatgg aagcacaaaa aattatacag ttacattgac tgcgccacgc acctatggag
    24361 gaggttctct tgtcatttgg ttgaaagcag actctctagc attgagtgat ggagcacttg
    24421 tttctacttg gccggatatg agtggttatg gaaatcattt tacacaggca actgcttcca
    24481 agcaacctac ctataggaca aaccaagtaa atgggcttcc tgcactacag tttcgacaag
    24541 ctacgattca gaatatggtg atctcatctg gtggagtgaa tttatatatc gatgacaatg
    24601 cctctttttt ctttgtcttt aaattaattc aaacaaatgc ggcaggaaca acgatcctaa
    24661 atataaatgg atcgaatgga cgagaaatgg ctttgactga tccgagtggt caatatctac
    24721 aatgtagaaa tggagtatct tgctcacaaa cttctggtgc tgtcattgat aggagttctt
    24781 tcgttgcgat tgggagtatt cagatacaag gtgctactgt gaatgaaatt tggaatggag
    24841 atttaaaggg agtgattgcg gctaccggtg gaaactttaa ttatgtgggg ggtgcctctc
    24901 ctggaacatc ttatttacca aatgggaatt tagacgctga ttttgccgaa gtccttttct
    24961 ttaatacagc attatcgcaa actgagattg ataaaatatt ttgttattta agagcaaagt
    25021 ataatttaat ttctacaaat actagctgcg gaatataaaa tgaacaatca acaaattgaa
    25081 attacatctt ctatccctct acatgatgag gagggatatt tatcaaaaga aggttgggcg
    25141 cgaagtccct attggcaata tgatcgatca aatttaaaag cctcaaaatt tagaatcaaa
    25201 gagtgggatt actactcagt tctgtcgcct gacggtgaat tcggaattac attcacaatg
    25261 agcgatttgg gatatgcagg tctttttgca atttgctttt tagattttaa gaaaaaaaca
    25321 tttcagcaag tggatactct gagtattctt ccattgggaa aaacaaattt tcctagtaaa
    25381 aacaatatag gtcaagtctc tttctctgat aaaaaacttt cccttaaatt tactatgaca
    25441 aaaggaattc gaactttgga gttttcttct cctttattta aagaggagga gggtgggaaa
    25501 ggattagaag ggacgatagt tcttacagag ccagaagact tagaatcaat gacaatcgct
    25561 acatcttgga aagaaaatag aagggcattt tattataaca caaagatcaa ttgtatgcct
    25621 gcttccggtg gttttagatt tggaaataaa acttatagct ttgaccccaa aaaaaacttt
    25681 ggagcacttg attggggaag aggagtttgg acttataaaa accgatggta ttggagttct
    25741 ggatcaggac tgattggaga gaaacccttt ggatttaatc ttggctatgg cttttcagat
    25801 agaagtccag ctagtgaaaa tgttttaatt tatgagaata aaatccataa attggattta
    25861 gtttcttttc agatagatac aaaaaactat ttagctcctt ggaaatttac aagtaatgac
    25921 aatcgtttgg atttagattt tactccgatt gtagaccgat cttcctgttt aaatttaggg
    25981 attttgaaat caattcaaca tcaggtattt ggattattta atgggagtgt ttttttagat
    26041 tcgggagaaa gagtagagct gaaaaacttc ttgggatttg cagaagatgt tttgaatcat
    26101 tattaatttt tactctataa aatcattgat cagaaataaa cttttctcta taaaattgat
    26161 cccatagagt cttccattta ggaaccatcc tactactgct ctactctaga tgctctatgg
    26221 ggggaaatgc cgatttgatt gcttttaacc gctagattca ctagaaaaat ttctagccga
    26281 agcattccct ttttgaaaaa tggagtaata tgaataaaaa gagcattcga acttctgtta
    26341 ttatactact tactttagcc ttactttctg ggggattctt tggttatatt ttatcggaag
    26401 tagatagagg agaggagctt acaaaactgg ctacctatct accaactact cctacaaagc
    26461 tttatgatct aaatggaatt ccatttgcag aattgtatag acatagacaa gagttactta
    26521 gataccaaga tataccgcct cacgttattc atgcctttat ttctgtggaa gataataatt
    26581 tttacaatca ctttggaatt gattttaagg cgattattcg agcgggattt attaatttga
    26641 ttcacttgaa agtgaagcag ggtggttcta caatcacaca acaattatcc aaagcaattt
    26701 taaaaaattc gaaaaaaagt tttactcgta agttcattga agcgttactt actctacaaa
    26761 ttgagcagga atattccaaa gaagaaatct tagaaattta ttttaactta gtttatttag
    26821 gtcatggatc gactggactt tctactgcgt cttctgtcta tttttcaaag gatgtgcaag
    26881 atttagatgt ggcagaagcg gctatgctcg cgagacttcc taaggcgcca gttcaatatt
    26941 cgccttataa aaatccacaa gctgcaaaga aagctcacct aacggttctt cgtttgatgt
    27001 ctgaaaatgg atttcttaaa aaagaaagta tccaagcaat gcatgaagaa ttttggcaaa
    27061 gatattggcc tatcgtcatt actcaatccc catctcgatc tacatgggga accaaattag
    27121 atagagctcc ctattttaca gaacatgttc gtaagcaact tcttgctgat ttaggagaag
    27181 atttagttta tactggtggt ttaaaagttt acacgacact tgaccttcgt aagcaggaga
    27241 ttgcagaaga tgaggtgaaa aaagcattaa cacattatga taagacatct tttggtttaa
    27301 gcaattcttt taaagcaggg gtggatggac aactcgttgg actctatgga cttttgggaa
    27361 gtattttccc tatcgctcca atggagatta gtaaattcga tgaagcggct aattatcgta
    27421 atacgcttga gaatgaactt agtgatgcac ttgatatttt gactgccatg actccaattg
    27481 agaatgaggc ttctgcaatt ggtgaattta gaaaacgcac tgcagtgttt actaaaaatc
    27541 tacacgtaga gggtgcattt attacaatcg aacccggaac aggttacatt caaacaatgg
    27601 taggaggttc aaagtttact cctaagaacc aatttaatcg tgcaatgcaa gcaagacgtc
    27661 agacgggatc ttcctttaaa ccttatgtat atggtgctgc tatcaatgaa agagtaattt
    27721 ctagtggaac cggtataatg gatgctccgc tcactacttt aaatgatgag gggcatggtt
    27781 gggcgccgga agattctact ggagatttta aaggaatggt tcctgcatat agagctcttg
    27841 ctttatctct caatattgtt tctgttcaaa ttttctttaa agttggggga cctgctatca
    27901 ttgactttgc ctctaaattg acaaaagcaa ataagcgtcg tttcccggat aacccaactc
    27961 tagccttagg agttgcagaa cttactccgt atgagatggc tcttggctat tcgatcatat
    28021 caaataaagg cagagatgta attccttttt ctgtgcgtta tgtgaaaaat caaacaggac
    28081 aaatcgtttt taataaagag ttaattgttc aaaaggaact tgctgctaaa gttgcaaatg
    28141 gaacaattca agttattcca gaaagcactg cttggattgt aaggaaaatg ctacaagggg
    28201 ttgccgatag tggaactcct acaacggctc taagagaagc aaaatacaat ggcgtttccg
    28261 gcggtaaaac tggttctaca agttctttta cgaatgtttg gtattgtgga tttgatccaa
    28321 aacttacttc tgtgttctgg atgggctatg ataaaaactc tatttcactt ggtcctggta
    28381 tgacagctgc tttaacagcg gttcctgttt gggggcgaat ttatgcaaga tggtatgaga
    28441 atcaagagta tccacaattt aagaatgcag atggagcaga tccaatgcca gaaggagttg
    28501 ctgctggcgg agtttgtgcg cataacggtt tgtctcctaa gcctggagtt tgtcccgttg
    28561 ggggagcact ctatttaaag cctgctatgg tggatggagt tatgcgatct gttccttgga
    28621 atagacagtg tgatggagat agagatcacg ataaggcaat tgattttaga gaatttttac
    28681 aagatgaata taagatctca gacgaagaga ttgagaaaaa taaaaagcca tttaaaataa
    28741 aagctgatta atgaaagacg ataattacgc agttctaaga atactagact ttagaaatta
    28801 tgtaatcgca cgatttctag cagttgtagg aattcaagtg caagcagtcg tagtcggctg
    28861 gcaggtattt gacaaaacaa atgatccatt agccttagga ttagtggggc tatttgaaat
    28921 tatccccgca atctctgttg cactctttgc aggacattta gcggatttaa aagacagaaa
    28981 gacgattgta ttgatttgtt attttgtttt acttttttgc tctgcctctc ttttgttact
    29041 tagcacttcc attctaaatt taccggcaaa caatgtttat ccgatctatg gaattatttt
    29101 tatcagtggc attgctagag gttttttaaa tccaacgctt gctgctttta tggctcaatt
    29161 agttcccaag cattattatc caaattcatc ggcttggaat agtattgctt ggcaagtagc
    29221 tgctgtgtgc ggacctgctc ttggaggaat tctttatggg ttcaatggac cgattctagc
    29281 ttatgcagta gatgcatctt tattaattgc ggctattgta ttttatatga gaatacttcc
    29341 aaaacctcta cctgagagaa agcaaggtga gtctattttt gaaagtctat ttactggatt
    29401 tagatttgta ttttcaaatc aagtcatgct tgctgctatg agtttagata tgtttgcagt
    29461 attatttgga ggtgcagttg cacttcttcc tgtatttgca aaagacattt taaatgtagg
    29521 acctgaaggt ctaggcatac tcagagcgag tccagctttt ggagcatcta tcatggcgat
    29581 cttacttgct cataaaccac ctcgtgaaaa cgcaggacaa attttattga ttgcagtggc
    29641 tggttttgga gtatgtatgc ttttatttgg tatatcgtct aacttttatc tttctgtttt
    29701 cttattaggt atgagtgggg tttttgatag tgtgagtgtt gttcttagat caactattat
    29761 gcagacattt acaccagaga ctatgagagg tagagttgcc tctgttaata gtattttcat
    29821 tggatcatca aatgagttag gggctttcga gtctggggtg actgcaaaaa taatgggagc
    29881 tatcccgtct gtcgtttttg gtgggattat gactatgttg atcgtaatga taacaggggc
    29941 ttttgcacct aaacttagaa aactaaattt taaagaggaa aacagtgatt ccaagtagtg
    30001 atttaaaaga aataaattct tttttaaagg gactgaatgg ttcaaatgct ccttttgata
    30061 tttacgtaga taatcttcac tcgccctatg tattactcag agaagaatat cttctgcctc
    30121 ctatttcaaa tatggatttt actaaaaaag atattcttcc atttttaaag gtaatatcta
    30181 aatacattcc agaggcgatg gtaggttgta gtgttctacc aatacaaaaa cctaagagag
    30241 aaactgggaa aatatcttta gtaagagaaa tctcgcttcg tggtcataat tatctctata
    30301 tttttaagat tgacgctgct tatttaggag gtagtgcgaa agataaaatc atttcggctg
    30361 cttcgcaggt ctctcatcct tctttttcaa ctgatcgtat ttatttttct acaaagattg
    30421 ttcctatcaa gaatgtaatt cgtgcagatg gggaaattat tgattttgaa gtgcagactt
    30481 ttgataaagg tttatttatt actgaattgg aagaaacgat agatgatcga cctaagaagt
    30541 attcagaatt atttgatgaa attgactact cacctgtgat ggagccaata aaattaaatt
    30601 taaaaattat gcacccaaac tggtcacttg gaaaaatata tgaacctgtt tatatagagt
    30661 atttaacttt atccgttcga tttcttcttg catcctatgc gaatacaata aagcattttt
    30721 ctcatttcta tgaaatctta gagattattc atataaatgg ttctatttca gaaatcacta
    30781 gaagaaattt catttactgg ttgagtaaac attcttttga gagaggaact tcgccctctg
    30841 gaaatatgag atggaaaatc cttcaagatc aaatttaaga atatgaagaa aattttactt
    30901 ggaatcattt tatctatacc gctctttgct caaagagcag acttttatga tacatataat
    30961 tacgatatga gaaatatgaa gttaggcgac tcaaaggatg gagtttaccg agattatgac
    31021 atgcctccaa aatttcagaa acctccgctt gtaaatccaa aaacagttca acgacaacct
    31081 gatattagta gtttgctcca agggcaaaca agcatacgtt cttcaaataa tcaatcccaa
    31141 aatcctgtcg catctcagac taataatctt cctattgatc ctgtcactgg gaaaatcaat
    31201 ccagatgcaa ttgccagaca aagagaaaaa gagaaacaaa acaaagaaaa aaagcgacag
    31261 ttcttaaata gagagataga gccttataca gagactagag gtcgccgaat ggaaatcata
    31321 tttctcacaa cgtttccatt tgctgctgca ttttctgttg gagtcacagt agtcatcgga
    31381 agcatcatgg gtaataacac ttatattaaa acaactcctg gttttttcat ggcaatatta
    31441 ggggcgacag gcctttctgc tgcaaacatg atttcggata taaatagcta tgatgaatat
    31501 atgaaaaata aaaaggaaac aaatccactt cctccaccac cgccggaaga aacacaaagt
    31561 caaatttaca aaggacaacc aaattataat ttttcctttg tatttggggc aaagaatttt
    31621 taatgattcc gaattttcta tgaatctata aaaaaaacgt atgaatcatc atctctgttt
    31681 catacgggta aatgaaacat ttgcatgcat taaaagaaat accgataaat attattccta
    31741 tactacctta tcaggtaact ggttaaatct attttaaata ggaaatgcaa tgagtttagc
    31801 tttggagtta acgtcaaggc tagagtagtt gttttagaaa tgaggtttca agatgtttcc
    31861 aagagatgcc tagaaagtta gaaaactctt tgtttactca tcctgcatta ctaattgtat
    31921 gatttgagct tcacgagaag ttaaatcaaa gttttccatt atacataccc catacctctg
    31981 gctttttgca tgagctgaac acgattggat acttctagtt tagaatatat attgttgata
    32041 tgtttacgaa tggtgccttc tgaaacagag agtaatttag aaatttgagt aacactacga
    32101 cctgtgatga ttaactccaa gatttgtttt tcacgagtag aaagggatga atcagaattt
    32161 tcttttacct gagagcgaaa gtattgagaa atatgaatgc cgatcgaagg agacatatag
    32221 gaaccgcctt ctaagattgc tagaacagag tcttttatat ttttaatttc atttttccaa
    32281 atataaccgg atgccccaaa ttttaatgcg ttaaatatca tatcatctgc attgaaagca
    32341 gtgataatta tgattttaag ttttggatat ctagaatgga taagttttaa aagatcgatt
    32401 ccattcatat gtggcagacc aatatcgatt agaagcgcat ctaaagattc aacgcgatgg
    32461 tcattcaagc attcttctac tgatctttat aaattgttca atttatatgg aaaattgatc
    32521 tactcattat aaatgtattt atctataatt gaatattatc tgatagctta aaaatgcttt
    32581 ttagaaaatc gtctgtgagt atatttcgaa taaggtcttt ttcttctctg tcaatgattg
    32641 tcttttgacg atagagaatt tgaactatat ctgaaatatt gtctttccct ttgagtgcat
    32701 tgataattcc aatgattcgt tttaagtttc gttgactaca tggttttttc ttttcttctt
    32761 ctaaatcaaa tattgtgtgc actttctctt ttattgaaag cgttagatct ataatatcta
    32821 aatctgtttt gaatttttct acaaatctct catatttttt ttgatgcagt tcggagcgat
    32881 ccttttcctt tttaaacatt tgcagaatat aattatacag ttctggattt tttataaaga
    32941 aggattttat cattgtatct tctaattcac taaacaaatc ataaaagaaa tttgtttcgt
    33001 cttctctatt tccagtttgt tcaaagtaaa cattaattcc attttcatct aggttggaaa
    33061 taaaggctag cgcaaggttt acatcaagat ctacaatgtt ttcctcaatt agaatttgta
    33121 aacatttctc aggactaaag agtgttgcaa tgtccataaa gaattcatct ggatcattgt
    33181 cctctactgc aatccaacgc cccagagcat aatagataaa ttgaatcaaa aacttagttg
    33241 gaaaaagtgg actacttaga atttgctgga aatcggaatc atctctaaaa agccaaagat
    33301 aataattcat ccattgttga aaggatggaa actgttgatt tttttttact aaatcaatga
    33361 attcctctgt tgagaggctt actaaaaatt gtctggttgt gttgattcca tgaatttttt
    33421 ccggaagccc aataacctgg aagctatcaa tttctatagt ctgattttga ttttgaattt
    33481 ccattgacgc acaattgtat tccttctatt ggaattgtat attattttta attagaataa
    33541 aaaaataatt tcatttcaat atgaaataaa attcagtgaa agagtaggga taggtcttgt
    33601 ttgcaatatg tttgaaaata gaaatagaga taaaagaaaa ttaattgtat tactttatag
    33661 aataaaaaac aaatttttag acactagaaa ttcaatacat aggtttcatt atacatacac
    33721 aaggaatata acgcgaggca tctttgctct ttgtggattt ttatctattg caacacttac
    33781 cattgaatat ggatttggat acaatcccaa atggggctct acaatatggc taattaatta
    33841 tttagccatc caatatttgc tcgcatatga aatattaggt cttgttttta caactgatca
    33901 atataaaaat tatattaaaa atcataaacc tgagataatt attgttagcc tcgttttatt
    33961 gcagaagata tttgaacaag atattatata ttatttaaaa ttaggcgaat ttggcacgaa
    34021 tgatactgca cttttgtttt tatctataaa tcaaatcttc ttagtttttt caaatctggc
    34081 tagacttgtt cgaaatacaa gactttataa tttgaagaag ttgaatccat ctctaatttt
    34141 cttttttagt tttgcttcta ttggtctaat tggtactctt ttactttctc ttccgaaggc
    34201 acaaaagata gaaggatctt tgcggctaat agatattatc tttacagtag tcagtgccac
    34261 ttgcgtaact ggtttatcga cgattaatat ttttgattcg tttactacaa tggggcaagt
    34321 aattatatta atactcattc aagtcggtgg acttggtctt ataacactta caacattctt
    34381 ttcaattttt cttgcaggac aagcttctgt aaatgataag cttttaatga aagacttatt
    34441 gagcgaagag gcaattggga gggtaaagcg aattatccgt caaattgcaa ttcaaacctt
    34501 gataatagaa gcagttggtg cagaactttt atatacatat ctgcctgagg atgcagattt
    34561 aaaaggtgga aataaaatct ttttttccat ctttcattcg gtatctgctt tttgtaacgc
    34621 tggtttttct ttatttacga atggacttgc ggaaaatttt tttctacatg gaaaaatata
    34681 tttagttgtt ataatggttt taataacatt tgggggactt ggttttcctg ttgtgagtga
    34741 agtgagtaaa aagttattta ctcctgattc tagtcatatt cgtctgtctg tttcttccaa
    34801 acttgttatg gttgttagct ttctactatt tttatccggg actcttgctt attatttttt
    34861 agaatcaaag catactttaa aagaattgga tcagtttgat cgtgtgtttc attcaatttt
    34921 ttattccatt acctgtcgaa cggctggatt taatacgtta gacatcggac gtatgggtgt
    34981 tccaatggta tttttttctt ttcttctaat gtgggtgggt gcatcaccta attcaacagg
    35041 tggagggatc aaaactagca catttgcagt cagcttacta catattgtcg atcttgtgcg
    35101 aggaaaaaat aaattagaaa tatttaatag aacaatttct cctggatcaa tttctagagc
    35161 ctctgcgact attgttcttt ctctttttgt aatattcatt gcaatcttta caatgatttt
    35221 aatagaagat tttccttttc tcgatatttg ttatgaagtg gtttctgctt ttggaactgt
    35281 agggcttagt cgtggcatta ctagtaatct atcgacaaca agtaagattg ttatttcgat
    35341 tgtgatgttt atgggaagag taggggttct tacaattcta attgcattca caccaaaatc
    35401 gaaagcgtat aattatacat atcctgtcga atacgttgta gtcggataat aaaggaacta
    35461 tcgttacata atttgatatt gtattaatga atattttaga aaaaggaaaa tcagatttga
    35521 gtctagtatc tcttgctact tgttattgga tttaaattta gttgaatcaa taatttcaat
    35581 gttaatttag ctttaagatc agaatagcta atcgcaattc taaaatacaa ttatctaata
    35641 ttcacaaata aaatcgattt atatagccgt gacgggatta tatttgagag aatagatttt
    35701 cgctagttct cggtctggtt tttaacagag tgaggttttg aaaaaccgaa ggtgattgca
    35761 taaccaaagt agctttttat aaagttgaga ccaaagatta gaaaaatact ttgaaggatg
    35821 gaaaaacaaa gaaacccctg agagactcag gggtataata attaggtgta ttagttttgt
    35881 ggtgcgttga aaaagtcgct agcgttacag cctggttgac caacgtctgt tatatttttg
    35941 gttgctttat caaagcagaa catttgccac caacggctaa cctgagcagg tccaacattg
    36001 tatgttctta ccagaccagt tgatttataa actttaacag tagaacccga aactgagagt
    36061 gaagcttcat ttgaccaatt aaagagacta taaccaagtc tagttacaac aggagaagtc
    36121 caaacagttc ctttcatagt tacagtctca ggaccatatc cggttgttac gtctatatct
    36181 aatgttaagt cttctgtaga acactgagca ggtgatttaa taactgcatt gtttacacca
    36241 gggtttctac acttgtcatt ccaaacgact tggtttttcc ttgtggtaga tccgtctcca
    36301 tattctaaat gggagtcaac atccttaggt gtagcattcc aagaaagaac gatacgaact
    36361 tcgtcggcag ctaaactagg agaaactaat atgttttggt ttgctgaatt tgcaccagca
    36421 gatataaccg tttcatatgt tgtaacaaat ccaggaccag taatttcgat tgtatagtta
    36481 ccggctggaa cgttaggtat tacataagac ccatcagctg ctgaagtaat agctccaatt
    36541 gtttggttgt ttgcatccaa tgcataaggt ccggatgtaa ctccaatgcc tggtctaatt
    36601 ctaacagtca aaggtgttga aattgcttga ttgttgagag cattcaatag tcttccagag
    36661 attgaagctg gtgcttgagc tcctggtatc atagttacaa tatcaaagtt agttgtagca
    36721 tttggttgaa tcgttacaat gccttctact ggcacataat ttgccgaagc aaatttcact
    36781 ttccatgttc cagggttgat agagaatacc gaataatttg gaacgtttgt aaatgcagaa
    36841 gttccagaag ataaacttgc tggggctact ggacttgttg gtcttgttcc tgtattcgga
    36901 tttacgatct caagtgtata tgttccagta aatggagcgc ctccagcgat tacaatacga
    36961 ccagagagat ttcctcttgg tccatctaga acgatttgcg cgttagcaac aacagctggt
    37021 ccagaatttg gaacggttac ttgaacaaac tgtgttacat aaccgggtct tgttactctg
    37081 ataacgtaag tccctggatc aatattacta attgtatagt ttccatttgc atctgttgtt
    37141 acggctggaa cttgaatatc agatgcagat aatctagaac cagatagtgt tgcaggatct
    37201 tgacgaaccg gtccccaagt aattgtattc gggtcagaat ctgtatccat tcctaactca
    37261 atggttgcac cgcttaacgc accagttgaa attgcatctg tgattgttcc agtcacttgc
    37321 attctaggaa ggtgaggaac ttggcttaca acagcaagtg ttccacccaa agcaacggtt
    37381 ccactgttta ctatcgctcc ggtagaggaa gatgtcatat gttttaaagg atctgtcact
    37441 atgatattgt aagaaccacc tctaaattgg tatcctgttc caggacaagc agctccagag
    37501 caaatagagg aagttacatc atttgcaact aaaccattat ttggtgttgc ggacaacgta
    37561 ctgagtgttt gagcggaggc atattggctg atagcatagg ctacacgtaa attatctgcg
    37621 actcctgtgc aagaagcacc tgataaagaa gtaactccag gatataaact ggaaactaac
    37681 gcagagttag agcaaagaac cgttatgtct ggaagattta ctactttcga gtttccatca
    37741 aatgagtatg ctccagatgc atttgttaca gttatatgtg ctacaggatt tccgctgttg
    37801 tcggaattcc ctaacataac tgcaaacatc cctggcactc cagattgagt attgaaacca
    37861 ggaacagccg caccataggc gttacgtgtt ccattcgata aagtatctaa gactacggat
    37921 tgacctgcga tttgggctct atgtgtagaa ggagttaaaa ccacaggggt agcattgact
    37981 tcttgaccat tgagggtaat tgtgcttgat gcagagttgt tgtagccggg agctgataca
    38041 taataactcc actttccagc tacagctgtg aaaaatacat tatttccatt tacctcataa
    38101 actttatttg cataagtgga ataggtccaa gaactgccag attgcatatc ggatcttgct
    38161 ccaatacagg ttggtgtaaa ggttggacat gcagttgcgc aagcagaaac ggctgcacaa
    38221 gcttctctca tccaaagaga ggcaaggtta cttaatctta cgtcagatcc tgtagccaaa
    38281 tagagtaagt ttgatggagc ttgtgtgcta tctggtctaa agtttataga agcacctaca
    38341 attctaggtg gagtattgct aggtcctgtt acttgaccaa ctacgttgga aggatttgga
    38401 actacattat ttggacttgc ttgggtaatc gatgttgcac ttccgtttgg atttgcagta
    38461 aaagaaaagg agtttgatgt agtcataaat ccggctttcg aaatttcaac ggaataaact
    38521 ccacttgata aagtttgact aaatgaatat tgacctgatg cattcgttgt agtagttccg
    38581 acaacattcc catttgcatc ttttagtttt acggtaaagc cagaaagatc tgaaccactg
    38641 attgcaacat tgcgtatgct ccagttggag atatttgcag ttgcagaggt agcaggattc
    38701 caagcagaac tgagaccagg aattgtcacc tgagtaggtg tcgctggatt gtttccttga
    38761 aaactgaatt gaattggaac agcagaacta acatctgaga aaggctgacc attgctggat
    38821 tgtcctgatc ctataacggt tatactgtaa tttccatttg gtaaattcgg tatgttgaat
    38881 gtataagcac cgcttgttgt agtagtagtg gcaatcacat tcccattcgc atcttttaac
    38941 tgaacaactg ttccatttgg cataggacca gcggcaacaa ttgtattccc attttgatct
    39001 ttgaatcctg gtgttgttgc agttccagtg attgttgctg gacctgttgt aaggtaaagt
    39061 ctagatgaag ttaaatctat accggttaga gtgttagcag atgggttaac tggattgtat
    39121 gtaaaattta catcttggta tgcgtagttt aatccattcc ctgtattaat caatacacga
    39181 taatcgccat tacttaaatc tgcaatgtta aatgtatact ttcctgttga atctggagtt
    39241 gtggttgcta ttacatttcc attgctatca actaattgaa ttgtgatctg agttaaatct
    39301 agaccagagt cactagagca ggcaattgcc acgttgcttg catttttaca attggttgcg
    39361 tttaatgttc cagaaatagt agcaggacca tgggttgcta cttcttgtgt tggtgcagtg
    39421 gatgggtcag atgagttgga aggtagagga acaccgtttg agtcggtttg ggaactgcta
    39481 ccactgcctg tgccagttcc tacattggaa ttcccacctg tgagagctgc catccagaaa
    39541 aaagctttct ttttcttttt gccacagcct gcactgaatg ccaccgctag tgctagaagc
    39601 aaatagaata agattctagt atagctaacc ttgcctttat ttgtatcatt tttcatttta
    39661 gattctccta atttttcgtt taaagttttt gcttatctgt agagaataat tttccctaca
    39721 gaattctaag actatcgaac gaaaaaatat atgtcaattt ttttttactt ttttccattt
    39781 tattaaaaaa aaaagttcat atgcttgttt atctgaagtc tgataccaac aatagcccat
    39841 ctcctgctgg aaaaagggtt ttgctgcaag gtcttttttc tattagatgc caaaattctt
    39901 gcatagctct atcagagggt tttgtgtaaa tagaaccatc taatcttcca tgccagagga
    39961 cattatcaaa aagaagcttt gttgtttgat ctgacttttc tattaatatt gcaaatattt
    40021 cgggataaca aatcttatcg caatcaataa atataaaatc ataagaaagt tgttctattt
    40081 tttttaaata ttcaatacaa tgtgtatgga taaaatttat cttttgagtg tgtgttttta
    40141 tttgatctag aaacatatgt gcactctgga gttcttttgc gtttcgatca attgtatcaa
    40201 tttccaatgt attgcctgaa ctcagcatcc atgcggtgga ataacctagg cctgttccga
    40261 gttccaagat tttagatgtc ttataggttt cacatagaaa ttttacaatc gatcctgcag
    40321 ctggagacaa aattggaatt cgattttttt cagcaaatac ttccatctcc ttcataattg
    40381 gaaatggtct atcaatgagt cgatcattga taaaggattc gaaaccatca atataaatac
    40441 tttcaaattt tttcatggaa gaaaacagtg aatacgaccc tttaagcttt caggtgtttt
    40501 ttttcgtatc atatccttga tataactatg tttataacgt tttgaaaagt gaatcagaac
    40561 tatcttttca tttttaaatt cttttgcatg cgccattatc tcatctagat gagtatgccc
    40621 ccaagctcta gctctttcta catttctatc atcatctaca taagtgcatt ctaaaaatag
    40681 gatcttagat tctctgacgt ctttatgttc taaaacatat tcaatctgcg tgtctccaga
    40741 gaagctaaca agagggagtg atttttcttc tgttagattt tctcctttcg atttcaattg
    40801 cacaatttca gcactcgaaa gacttgcata ttcagattta atttttttaa ctttttcaaa
    40861 gattgtataa ccttgagatg gtattctatg cgttgtttga tatgccttaa aaaaagtatt
    40921 cgctgtaagt gaaaattctt tattcgttgg agttgaaatt aaattgtaat tgtaagagaa
    40981 gccttcaatt tctgaataga gttctattat tttttttgtg ttctcataca ttgcttcatt
    41041 tacataaata tttggagctt ttagtttttg gagggaacgt tgtgagatat aataaggaat
    41101 tcccgctgca tgatccaagt gtccatgagt gactaaaaca ttttccatat gaattttttc
    41161 atgaggaatt ccaccgatat caaatagaag gctatacctt ggaatagaaa tactagttcg
    41221 aattccccct tcggaaatgc cctcaaaatc tatatttgca tgtgcgaatt taagatacaa
    41281 aggattactc tacactatct tctggtaaaa ttacttttac attttcaagt ggtaatttat
    41341 ctgcagaatc tttattggtt acaatgactg tttttagttg atcaaatgta aaatattttt
    41401 tcccaacacg taagatatca gctagagtaa cactgtttat gtttttttga taattctcta
    41461 aataattctc aggcatttca tcttcatcaa aagatacttg gtttgaaaca atgcggttgt
    41521 tatttacaaa taaaaataca aattgattgt ttatagatct ttttgcatct tcaatttcct
    41581 tctctttaat ctttgcaaat gtctcttctg agaggattgt cttcattaga tcatggactt
    41641 gcaataagct ttgattctta gttagtgtat atgcgtataa gattccatgc tgctttttaa
    41701 aaattggata actagatgat gaataagcaa gacctttatc tacccgtatt tgttgcatca
    41761 tataactatt aaagccaccg cctccaatga tataatctaa aatttgcaac gcataaaaat
    41821 ctttatcgtt atgtcgagga agtgagccaa tcattaatac catggattga tttacatctt
    41881 tggtgataat tagattttta tatttatatt cttttagaga ttttgaaagt atttcttgtg
    41941 atacattttc gcgattgatt ggtttattcg aattagccgc tgggaggatt tcgttgaatg
    42001 ataattttat tttgttagag tcaaaaagac ctgtgaccga aattcgtttt tttcttgatt
    42061 gtgctaattg ccaaaagctt tttaggtctt ccatttttat attttcgatc gaggttgctt
    42121 gagacacttt tccagctaga agaccacggt aaaataattc tttagctttt ctaaatccta
    42181 aagaatctgt tctttcattt cttcttgcaa tcatttctat cattttcttt ttcccgtttg
    42241 taagagcttg ttcactgaat acaggatttt gaatcagatc ttgcagaata tctaagatcg
    42301 tattttcatc ccttgaaaga tacgatacat tgattgtaat tttgtcataa tcgcttgaga
    42361 cagataatcc ggcacctaac gattctagtt ttgatagcat tgtttcttca ggatattttt
    42421 tagatccacc aaagcgtaga acttctgaga gaagagctgg aatttctatt ccattccctt
    42481 ctgtatctgc ttctccagca taaaaagcga tttctagata ggtaatagga aatttgtctg
    42541 agtagatgga gtaaaatttt aaatttggat ttagatcttc cttactaact tttggaatat
    42601 gaaaatctaa ttcgtcaatt tttatatcct tcacaaaatc ccctggtgcg gctaatagcg
    42661 atattccaag aaagagtaga aaaagaaatt ttagtttaaa atttataaaa tttagaatta
    42721 gtttcatttt gtgttttttc ctgtttgtgt tgattttgct ggagccatat aatttcttga
    42781 atctaaatgc cctactgtta gattttcttt tacaaagtat ttacttacaa ctttttgaat
    42841 gtcttcttct gtgatattgt taagcttatc ataggataaa aataaatctt cccaagttcc
    42901 tgtgaggagt tcatagtagc ttagattatc tgctagactt ccattattgt ctatatctct
    42961 tagaaaatca gcagtcattt tattcttgat tctctgtaat tcttcttttg aaactggttc
    43021 ttttttgagt ttttcaattt cttcccatat gatagattct actttgttag ggtcggcatc
    43081 tgaattgagt tgtgtataga tgctaaacaa atttgaatat ctatctcctg ggtctgacgc
    43141 ataggcatct acgcttgtaa ctaatctctc tttgagtgca agtcttctat acaatctact
    43201 gcttgtcccc tgtgtgagaa tagcatcaac taaatctaat acactgctgt ctctatttgg
    43261 ataggcaggt ttatgccaac ccattacgag gatggaacca cctggataag caaaggaaat
    43321 tctcttttcc cctgtattca ttttttctct gactctagtg cctaacttct tttctgatga
    43381 tggttttaga tcagagaaat atttttttat catagcttct gttttatcaa agtctaaatc
    43441 tccaacaata gcaattacca tattgtctgg agtgtaatat ttcttaaaaa agttttctgt
    43501 ttcgtaaata tctaaaaaag gaatatttga ttcataccca attacaggct gaccataagc
    43561 atgctctccg aaagcaagct ctaagaatcg ttctcttaat aatccgattc ctctattttc
    43621 tacccgcatt cttctttctt ccatgattac atctcgttct gtataatatt ctcgaagaat
    43681 tggattttta agacgatccg attccatttt tgcccagatc tctagacgat tcgctggaag
    43741 tcgaatttga taatttgtaa cgtcatgcga tgtatatgcg ttaaatccta cttgtccatg
    43801 ttgttcgtat atgtaagtat cttctgattt aataataaat ttcttttgaa tctcttccac
    43861 atctttgagt cttctctcta atcttttaat cctttctaga agttctttgg gaatcgattt
    43921 ttcttgatct tcaaaattct ttttttcaag tcttaactta tctagttcac tgcctgtcgc
    43981 ttttaataga atatgatatt ttttctcttc tgcataattc gtagtgccta cattttctgt
    44041 gcctttaaaa agcatgtgct ctagaagatg agctgttcct gcaatttcct ttgtctcatc
    44101 caccgaacca actttgaact ttgcatagag tgctagagta ggcgaggtat tgttttgcat
    44161 catgataagg cgaagaccat tctcaaattc aactttctta atggattttt ttagacgatt
    44221 ggctacgtcc tcaaagatat tttctgcact tacagtttgt gtgaatagta aaagaattcc
    44281 tagaaagact gatataattg atttcaaaaa tgcctccttg ttgtatgaat tacaatagat
    44341 attggcaaga ctttctattc aatctatttt gacaaaaaag atgctctcaa aattagataa
    44401 atgatttaaa gatttattgg catataaaat cgtggaaagg cttggatagg acaatgaaat
    44461 tgatgttaaa cctaattatt tttacttttt tcttctttgt tttgaattgc agttctgttg
    44521 gggatatgta tgtaaatgaa gcagcagagg gtaatttgtc ggagttggag gcgattagcc
    44581 aaaaacatgg agaccagacg aatgcaagag aagagaggaa ttggactgca ctcattgctg
    44641 cttcgagcac tggtagggtt gatgttgtaa aatatcttgt ttccaagaaa gctaaactaa
    44701 atcttagaac gaatttgggg gatactgcaa tttacagagc ctcagccaat gggcacacag
    44761 aaattgtaaa ataccttcta gaagctggcg cgaatccaaa tattcgtgat aaaaaaggtg
    44821 attcctgttt aatgaaagcc gctagtaagg ggcatacaga gattatgcgc ttactcatgg
    44881 atgctaagat gaatccgaat gatgtaaaaa ttccgggagt ggatggaact accgcactta
    44941 tgacagcctc cttagaagat caagtggaat cgattaaact tttaataaaa tacaaagcag
    45001 atttaaatgc caagaatcaa aatggagata ctgcactaag ccttgccgca ggagctggta
    45061 aattgaaagc ggtgaagact ttgatagaag cgggagcgaa tgtcaataac gcatctaatt
    45121 ttgggatgac tccaattttg catgcagaag agaatggaca tagaaatgtg gtagctgagt
    45181 tacaaaaagc gggagcaaaa gaatgaagtg gaagttgtgg tttttatttt ttatctttat
    45241 aggatctatt caagcaggag taaatgaaga tttgttacaa ggagcgcaag aaggaaattt
    45301 tgcacttgtt aaaagatcaa tagaacttag agcagattta gattttagaa attcagatgg
    45361 agaaacagct ctcatcttag cggcttggta tggatctcct gaaatagttt cctatctttt
    45421 ggagagtggt gctaatatca atctacagga taatgctggg tatactgcca ttgcgaaagc
    45481 ttcttcgtta ggcgttgggc gacactatga aatagttgag atactgattc aagcctgtgc
    45541 aaatttaaat attaaaacaa aagaggggaa gagtcctttc ttgcttgctc ttttaaatgg
    45601 acattttgaa ttagcaaagg ttttaagaaa tgcaggcgca aaagaggaac cgtattttct
    45661 aggtgcagaa gcagataaag agttactggc tgcttcttct gttggggact tgcagcgagt
    45721 aaaatatgta ttttttttca aaccgaatgt gaatacttcg aatgaagcgg ggcaaactcc
    45781 tgttatgctt gcatctcgtg taggaaaaaa ggaaatttta attttacttt taaagtctgg
    45841 tgcgttagta gatacaaagg attattctgg taagactgct ctaatgtatg cagcaaggcg
    45901 tggaaataaa gaagctgtat ctgttttact tgattacgat gccgatgttg acatagagga
    45961 caaggatcat tttactgcgg ctacttgggc agagcgcttt ggacaaatcg aaattgcgga
    46021 atacttaaaa cgactgagtg ataagtaaaa ttagcaaatc caatttgtga tttgtgcgta
    46081 gccgaaaagc gtttttcgac cacgcacgct ataatgttcc ttattttgga gtagtagtag
    46141 cttgtttttt taagaaggca acatagtcat ctaacgataa aaatagattt gttttatctt
    46201 ctaaatcgct tgacgcaatg ataaagccca tatgttttcc tgctattata gaaatctttt
    46261 tgtttccaaa cagggctata attttttcga tgatctgtag ttcgtccttt atcagatctg
    46321 gcaagtctcg aatatctaaa ctaacactct ttggaattaa gctcttcaaa aaatctggat
    46381 tgaaaatatt tctaatttct ggcagaacat atttcattcc tgatttaaat gaaataagag
    46441 taattgtatc agttggattt tcaattacta tatgatgttt tctaaaaatg atattatctt
    46501 tcttttgttc cctagcaaga attaagaggc ttttgatttt ttctatgaat ttgactttgt
    46561 tgactggctt tacaaaataa tcgtgaactc cattctttcg tatactttct agtaaacttc
    46621 tatcattgga tcggattgtt ggaatgattg gaatgtcttt ggtgatagga tcttttcgaa
    46681 ttgcctgtag agcagccatt gtctctctat cattggattc gatcgcaatc acaatcaaat
    46741 cgggtaaaac agattttgtt agttctactc cttttttgac ttcatgaatt cttacagtct
    46801 tatatttata actgattaag aaggaatcta tgtcttttgc ttcttcttcg tttgcgtcaa
    46861 taataacaat tgtttgcatt tgtattttat tacctaaaat cctaacgtga cttaatctag
    46921 aatgtaaatt ctagaattcg tgtaatttat tgctatttaa aataattgca agtctttttt
    46981 gttcctattt cataagtttt attccaacag gtcgacacag tttgtttagt attattttta
    47041 caactggctt agaccccagc tctggaatga gaatataata attgtagatt gctctagaaa
    47101 ccattgattt tttatatatc tcccacgcga tttctctttt acttcctagt tcataagaat
    47161 agatatgtct gtttgcaatg ctatcatccg atccaaagct ttgaatggat ttaattctac
    47221 cttgttcatc aaaatttgta atgattttaa agatttgctt cttattcgcg atcggtttgc
    47281 ctttcgaatc aaagtgttgc actaaagtaa ggcaatcttc ttcttttttt gttttggaca
    47341 gacatgattt attgtattga tatgtgattt ttcctattcc ttcagaatta ttagcaggtt
    47401 gtagtttatt atttaaaaaa atttcctcac tcttataccc atctgcattg tagctataaa
    47461 tcatcttcgc ataacctaga ttactctcta tatattcatt gttagaatca aagttttcga
    47521 tcgaaagcaa tttaccattc ggaagaaaag ttctaacttg ctttgcaaag ccatcatcgc
    47581 cttcagttag ctttccctct aaattccaat attcctctga gatcatttct ttttctttaa
    47641 aggaggcagt gtaccttgcg taaccatccc tagataaaac aggcttatta tttatatcgt
    47701 aatattcttc tagaatttgg tttccgaaga gatcatattt ttgtattaga actgcatagc
    47761 cttctccaca tagctttgct ttgccttcag tatcgaagta ttctgttcta atttcttttc
    47821 cctctttgaa aagtttagta gatactttga cgatagccgc ctccaatact tcattcactt
    47881 ttaatttacc ttcagtatca aaaaaacgtg tagtctttgt atttgtgtct ccaatcatgc
    47941 ttgattcgga agtaatacga gcgatcccat cggcagggtc taattttaat ttgccgtcac
    48001 ttccatattg ctcttctaaa actaggtttc cattctctgc atattcgaag atatacttgg
    48061 caaaaccttt tccatcttct tttaactttc ctaaagcatc tctaaattct gttgcgattt
    48121 tatttccttt tttgtcatag agatggagag ttttggcgat gccttttgtg ttttctttgg
    48181 gttgaccctt tccatcgaaa gtttcgtgca atacaagtct tcctctttcg gatgtggtat
    48241 aatcataggt aaaagattct gaggcataac catcttttcc ttctttaggt ttttcatttt
    48301 ccccaaagaa actagacgtt agtttatttc ctctagcgtc atatgtataa acatattttg
    48361 caataccatt tgcatcttct ttgtattttc cctgtgtatc aaaattttct gtcgtgagta
    48421 gttttccact ctgattgtat ttgtatttga tctttgcgat tttattttta tttgtatttg
    48481 gttttttatt ttcgtctagg tattcttcct gtaatttcct tgcatctatg tcatacgtaa
    48541 tttttttaaa aatgggcttt ccattttttt tcttacctaa aaaagttatg agttcaaatc
    48601 catttttgag aattttaact ttgtttgtaa aaagtaaatt tcgatctata tcatattctt
    48661 ttaccttgaa tgtctttgtt ttctcatcgt atttaagctt tgcaatggca agaccttcta
    48721 gctcaacaag gtgctcacta ttttcctcta cacgaacaaa agaaatactc tttcgatttc
    48781 catatttatc gtatttaact ttggaataga ttgggctttt gccttttatg gaaaagagaa
    48841 tgttctcttc ttcttcggca ataatagagc tgagtccaat ggtgatgagt agaatgataa
    48901 aattccaatt tcgcagatga atgaatattt tcataaaagt aaagtctttc tgtaaaatgc
    48961 agatgtcaag gggatcatag aaaaaaaata aatttttacg gattctactt gcaacattcc
    49021 actaaacttt tttccattat caaaaaagat aataaacagg ataaagattt atgagtgtgc
    49081 aaacccttcc gttaccctta actaggcaga tttcttatgc cattggacag cttggctggt
    49141 cgattttaat taatatcatt ggctttcacc aagtgttttt ttatttacct cccaatgggg
    49201 aagggttacc agaactgatt gtaaaagttg ctttctttgg catttctacc ataggcgttg
    49261 tttctgcaat cggtcgcttg tgggatggta taacggatcc aattattgcc aatatgagtg
    49321 ataaatgggc ttctaaatgg ggacgccgta ttccattcct cgcaaccggt gggctccctg
    49381 ccgctgtctt ctgtctctta atttttacac ctccagaaaa agggatcaca actggaaatt
    49441 tagtttggat gaccgttgca ctacttcttt tttatttatt tttaactgtt tacgtcactc
    49501 cgttttttgc attgattcca gaattgggac atactccgaa tgaaagactc aatctttcta
    49561 cctttatttc tgttacctat gcgttaggta ttatggttgc cgcacaggtt cctctgattg
    49621 cagaaaaatt aatgaacgcg gttcctggaa tggataagtt tactggctat cagtgggcaa
    49681 ttggaattct ctgcaccatt gcggctatct gtatgtattt tcctgttctt gcaatcaatg
    49741 aaaagaaata caccaagtct gaaccttctg ctgttccatt taaagaagct cttatgatga
    49801 cttttaagaa taaagatttc ttattctttg cgatttcaga ttttaattat tttttatcga
    49861 tcaccatttt gacaacaggt cttagttatt atattacagt tcttcttttc caagaagctt
    49921 ctcttgtgag tgagatttta cctgttatgc ttttctcatc ttttctgtgt tatccaattg
    49981 taaacttttt tgcgaaacgt ttaggaaaga agagacttgt gatctatggt ttttttattt
    50041 tcttgctcct atttattttt atttatttta tgggaatgga ttcgatccca attccaaaag
    50101 taatccaagc ttacttgatc gttattgttg caggggctcc gatggcaatc ttaggaattc
    50161 ttccaaatgc gattttagct gacattgcag aactcgatgc actaaagact gggtctcgta
    50221 gggaaggatt gttttatgca gctagaactt tgatgcaaaa attaggtcaa accattggag
    50281 ttttaatttt tagtagtcta ttacttgtgg gtggagggat taataaagta gaaaaaatcg
    50341 atcctaccac ttaaaaaaag gttttagata gagaaaagtc cactgtgatt ggagtgcaaa
    50401 tcacaggtcc ggtcgctgct gctttttgcc tgctcgcaat cgttatgttt tcgagatata
    50461 aggagaaaga aactctagaa gagattgaaa ggattgctaa taaataaaac ctgacaatta
    50521 tacatatatg gaggacttaa ttatgaggta taaaatagag attagtaagt tgaagaacgg
    50581 ttatatcgag gctaaactta ttgatgttgc aacaaaccac ccaattgagt tcagaatctg
    50641 cgatacagag caatacgttc agtcacagat tgcagattgg cataagcgtt ttagaatgaa
    50701 tgagcccaag gaagatgaca ttaagaaata acttattttt tattttattc ttttttctca
    50761 ttcattgttc tagctttgga aaagttaaga acgattattt tgattattat gattctagtt
    50821 ttaaatgctt agcagaaaca ggttggcgag attctaatct ttactctcgc tatttagatc
    50881 tcggttggaa tccaattcga aaaaaataca acgacgacaa tcaaaaaatt aaatcagcaa
    50941 atgttgtaat cgttgggaat agtttgattc aattatttga tgaaaggctg atccaaagag
    51001 aatttccctc tctgaatgta gttgggaggg gaattgctgg tgatatgtca gagagtctgc
    51061 gccaaaggat ttccgacaat gtattatctt taaatccaaa aacagttatt attgagattg
    51121 gtgggaatga tattattttt gggaaatgcc tatcctatac agaaaacaat attcgaggga
    51181 taatagaaga tattcagagt tatgataaaa gaattaaaat tatcttttta tctgttcctc
    51241 ctacaaaaaa taaagtattg aactctgttg ttcctgcact caatgcctcc attcaatccc
    51301 tcgcaaaaga atataatttt gtctatttag atttgtggaa agctatgcga aacccagacc
    51361 tacctgtcat taaagaggat tttgttcgct acaatgaatt tggaaaaatg gataaaattc
    51421 attttaatga aaaaggttat gaggtcattg gtcaattagt taggcctctc attaaataat
    51481 gcttattttt atttcgcctt cagggaatcc aatttctata aaaaatactg taataaagcg
    51541 cttcggagag gcgaagtatc tttattctag ctctcttcat aatatttttg cgcattattt
    51601 agattttcca aacaaaatag agagagatga tttgattgct ttaagagagt ctttatcttc
    51661 tgaaagaatt gatgtccttt gggtagagga acttttagat agaagcagca aatcgctatt
    51721 tgcctttgat atggactcaa ctctaatcaa agaggaagtt attgatgaac ttgctcgtcg
    51781 caaaggtgta tatgatttgg ttgcttccgt tacaaaagag gcaatggaag gtaagttaga
    51841 cttcgattct gctcttagaa aaagatgcag ttatttagtc gggattgggg aatccgtctt
    51901 tgaagagata tatcaatcta ttacacctaa cgatggtgtt gttgaattaa ttccttggat
    51961 tcaaaaatac aaaggtattg ttgccatctt tagtggtggt tttgtgcccg tgattgaaaa
    52021 atttgctaag aaatattcgg ttttacatta ttctgctaat caacttgaga tggaaaacgg
    52081 atttacaact ggaaaggttt taggaaccat tgtaaacaaa aatagaaaaa aggaattatt
    52141 aatcgagcat agagataata atcaaatttt aaattctcaa acagttgcag taggagatgg
    52201 atcgaatgat tctcttatgt taaatgaagc cggtatagga attggctttc acgcaaagtc
    52261 cggtttaaaa aaggatatct taaattggat tgaccatcat tctatgcttt ctttgttatt
    52321 cttgtatgat ttacgcgatt aaattttaaa atggtcagag ttgccaccat gaagtctgct
    52381 gttttcaaaa ttatttttct acttttttta tttttacagt gttcttctgt aaccagttta
    52441 aaaggggtaa cttctaaaat ggaaaaacct gatcttgctc ctgaggttat tcctgttcct
    52501 gaagtgccac ttcaatttga tgaaaaggat tataaatcta gaaaggtttc aaagaagaaa
    52561 ccgcatttta catttgcccc gattcttttc ccagcaggtt ttaatcccga gttgatagct
    52621 gtatttcacc aagagaatcg ccttctttgg caaaatacag aatcaaaaga agtgaaaatt
    52681 tcaattgatt catggaaaag ccaagataca cttcgaaaag aatttgtaga acaagatatc
    52741 gattcatttg taaatactga tctcgctttt gatggagatg ttttaaaagt aaaacaaaca
    52801 ttcattgatc ctgtgaacca gaaggaatat ggaacgatag actttgagat tgccgtcatt
    52861 ttacctggac atcctgattc aaaatcaaaa atagaattat ataaaacaga gaataaattt
    52921 aaagctttag aagaaattaa atctccaatt ttaaagttca ttcgtaaacc agatcgtaat
    52981 ttacaaataa cacttcttcg ttcaacagtc tctgctcata tcaatgtttc ttcttcctca
    53041 agtgatactg ttttttattt agatggaaaa gaaatgggga aactcccgat tcgacgttta
    53101 ttaattcctg atggacctca ccaagttacc tatcgcaaaa ccggtttgtc tcctgtgaat
    53161 aaaaagattt tagttcgcgc gggcgaagag atgaatttat tccaagagtg ggaagatgat
    53221 ctgagtgtcg gatctcttaa aatttacagt tatccgaaag gtttaaaagt tgcttggaat
    53281 aaagaagtaa aaggagagac tccttttttc ttatatggaa ttaatccagg caagtatcgc
    53341 atggagttta caaagattca cgatactaag aaaataaata taccactcag agactttgaa
    53401 gtagaggttg aatctaaaaa taccgcagcg ttagtttttc ctatggagct ttacgattct
    53461 tttttaccat cagccattga tctctggcag ccggtgctag gtggagtgaa tgtcaattat
    53521 gcgggaaatc taagtcttga aaacagaagt ggacaggtca ttgataaacc aaaaggagtt
    53581 gtgacacaac caatacttcc agactcaatg gaaattagct ccgctttctt ccttccttct
    53641 gaaatgggtt ctggaaatat ttctttttct ttaataacaa agaatgcaac ctatacttta
    53701 gaggcagaga gagaaaaaat ttttctatat aaatttccat cgaatggagt ttcgattggg
    53761 gcgtatacat atgatccaca gatcaaagat caacatagag ctttccgtta tgtgacagat
    53821 atggataaag gaactctaca aatctatttg agtgatacaa aagtttttga tgattcaatt
    53881 gatttttcag atacttggaa gatttctgta ttgctccgag gaaatgcttc tccaaaggta
    53941 aatgcactca aatatctctg gattcagtgt cctaaataca ttcagctttt gaaataagat
    54001 attagttttg gattcaaatg gcaaatttgt ttggtttttt atttttaatt ctatttgttt
    54061 gttctcaatt tcaattttgt attagtatag aagagtctcc atctacaaag gcagttgaaa
    54121 ttactgagaa agaaaaaaaa gaaatcaaag cgagaaattt atttcatgag gggaatgatt
    54181 tatataaagc atctagtttt aaagaagcga ttcaaaaatt agaagaggct aattctattt
    54241 tgccgaccga ggataacttt tacctactag gtctttgtta tctaaaacta gatgactata
    54301 aaaattctaa ggagtctttt caacaggcac ttgaaatcaa tcctaaaaat gagagagtgt
    54361 tacttgcaga atctttactc gaaacagggc agggaaaaga agaggatagt ttagaaatac
    54421 tcacgcggct aacagagttt taccctgaaa aagatattta tagattcaaa agagctgtct
    54481 ctcttaaatc tctaaaaaga tacaaagaat ctttagatga atttaaaaaa ataaatgaaa
    54541 aagaattttc acaaaaatct caactcttta ctcatttagg agaactctat cttctcttga
    54601 aagataattc tttatcagaa aagtattttt ctaaagcgaa ggctttgaat ccagatttaa
    54661 aagatattga tgcttcaaaa aataaattaa gatatgatga cctaatagaa aaaggaaatc
    54721 tttactatta taaaaagaat tacaaagaag ccgctcatta ttttgaagag gctgttttta
    54781 tttcagcctc tgcctctctc tactttaagc tcggaacttg ctattctgaa tggaagaatt
    54841 tcaaaaaagg agaagagtat tttaaaaaaa gtttatcact agaaaaagca aaatcaactt
    54901 ttgtccattt agctaattta tatttaaagg aaaatctaat ttttgaaagc gtaaatatcc
    54961 tacagtcagc tactttaaac tatccagaag attctgaatt attttttaaa cttggctctg
    55021 cttacaagaa actagccaat cctaaattag ctatcatttc atttataaga gcaaaagatt
    55081 tggatcctaa ttcaatacta actagaaaaa atctggcatt tacttatttg gaagaaagtc
    55141 aattagtaga agcaagtcga gaatttaaag acttacaagc attatcccca aatgagtcag
    55201 agttcaagga tattctaaat attttagatg tgacagatgg taaagaggat gtcttagaaa
    55261 aaataaagaa atggaaaaat aaaagaacac tttcccaagg aagaaattta gaagatctaa
    55321 aacaatttga taaagctgag aaatattatc tatcaaaatt atcagattca atattaagag
    55381 atgctgcaaa atatcgttta tcgcttttat actcgattat ctctgaagaa gaattgagta
    55441 gaaatcgttt taccaaagca gaaatctttt ttaataaagc gaaagaatat gattcaaaat
    55501 ccattcaaat caaaaaaata gaaagtatac taagagatag caaaaaaaga gaaatcgcaa
    55561 acaagagtta tgcaaaggca gaaaaactta gta
//
LOCUS       DAOQOS010000018        55567 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_215_length_55567_cov_6.247802, whole genome shotgun sequence.
ACCESSION   DAOQOS010000018 DAOQOS010000000
VERSION     DAOQOS010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 55567)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 55567)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55567
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_215_length_55567_cov_6.247802"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..614
                     /locus_tag="PLF66_05850"
     CDS             <1..614
                     /locus_tag="PLF66_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004780492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FliG C-terminal domain-containing protein"
                     /protein_id="HMZ63599.1"
                     /translation="AVTLSYLQPKKAADVLKHFPQEMQSNVALKLAATSKTHPDAILQ
                     IAKVLKKKYDSRDKSELAQAGGAESLANILNFMDKNMEENILKELNEKSPDLAFQVRD
                     KLYTFEDILNLDAREMRTLVNRLGGNELLIVALRGAGDEIKRHFFTAMSQNRAADIVE
                     EMDSRGKTTLREITNARNDILRIARELEEEGIIILKKRKDEFI"
     gene            619..1344
                     /locus_tag="PLF66_05855"
     CDS             619..1344
                     /locus_tag="PLF66_05855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63600.1"
                     /translation="MNKKSNSTIDLLLKISNFSTIRFLFIFLILPFFLSAESIAELNK
                     KGILALQKKDYKKAEEIFKKSLSISPNDAKANYNLACTYALMEEQCEPSENGNPLTLL
                     EKAVLGDVYYKTKMLKDSDLKSLRNNIKFYKLAGLTKKQILTKIIWYGPKPGVANIDK
                     MVFTDSGKFYYSVFNSSGEGNPGYTEYNGRYVWIKEDIQIQFDESPFDSKKKIFNVKL
                     KENSIQIEGFDHELSDFEDRCSA"
     gene            complement(1356..3497)
                     /locus_tag="PLF66_05860"
     CDS             complement(1356..3497)
                     /locus_tag="PLF66_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020776836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HMZ63601.1"
                     /translation="MKPNKFIKSIVEYLKLKAGKEIAKSDLYKQFLETKEDKKHKKHE
                     GKRKRKYNDEKKAEIEELFWLLEKEDLVKLDKKTIQVKKPFSLSGIISISRRGDGFLK
                     LPSGNELFIPSNFLDSSMSGDTVEVTPLGIGKKERLEAEVIRILKRGRTLYRMRVTET
                     EGGYIHGKLLDMVGDDKEGLLSKKALLADIAKSIQINDILIVKLKEHAFQDGNTLEVT
                     FIKLESGDKEDIDFNRILMKYNYNQMYPDSIPLEFPEEVNEKTVIDWNSRVDLRDLYT
                     ITIDGETAKDFDDAISFIEEEKRIRFYVHIADVSYYVKPGSSLDEEAYSRSTSVYLAN
                     KVVPMLPPELSENLCSLVANQNRLAFTVEMEGDYSGKIYSAKFYKSIIKVNERYTYER
                     AEKEILEGDAKNWLFKVMRLANGLKKVRIENGRVDLNIKETYVVTDKDNRKVVEIKTR
                     ERLNSHILIEEMMLSANIKVAEHLRKNKVPTLFRVHESMEEDKIENLNEFLKLYGFKY
                     QLNSTEYDDIKKALSAIANHPAEKIFNYFLLRSFMQAYYSGEALGHWGLGFKDYCHFT
                     SPIRRYPDLVCHRALEALIHKEDNAYSSEEIKTMGYHTSTEERRAADAERDILKLKAC
                     RFVEATGLKEFTGTITGIKPHSIFVELDEILTEGVVSYTEFTNEYELDQPNDFSFYAK
                     RFTKTFFLGEKLKLKLDRIDYDEIKIFLKVV"
     gene            3955..7137
                     /locus_tag="PLF66_05865"
     CDS             3955..7137
                     /locus_tag="PLF66_05865"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HMZ63602.1"
                     /translation="MKTSEVRGRAPLAENGFLNTEQWDFQSEGTIKLDGQWEFYWLSL
                     LTPDDFRNLQTKNQNNLYQLVPSTWNQYNLNQEKLSGNGYATYRLKIKIKDIQRIYSI
                     KIVDSATSYKVWINGDLLATAGETAKTKEEMIPAYKSRVVEFKPTSDIIEIIFQVSNF
                     YHSKGGLWNAIEFGPAPLIEKFRESQVSFEFFMFGSLSIMAFYHFGLYALRRKDKSTL
                     YFGIMCFIVAIRILLTGERYFFKYADNVAQWILLVKLEFITVYLTTPAFIYFLSSIYP
                     AEAKKIISRITLGAGLLFSLFTIMIDTTLATQIIKYFQNLLFLLIVYTGYVLIKINLN
                     KREGAVWITSGLLVFLSALINDLLYLNNLIYTGFLFPIGLFVFVFSQSFILSMRFSKA
                     FTTVETMSDRLLALDKMKDDFLANTSHELRTPLNGIIGIAESILEDGSYNLPYEIQHN
                     LYLIVASGKRLNSLVNDILDFSKLKNREIKLHLRKIDLKQIVDFVIRLSEPLLLNRDL
                     KLINDIPSDFPEIEADENRILQIFHNLVGNAIKFTETGEIRIYAEIRNGVSTIYISDT
                     GIGIPKDKFEVIFQSFEQIDHSTIREYGGTGLGLGITKHLVELHGGKIWVDSTVGKGS
                     TFYFTIPSFTENDVRRDKNVLYTNADFYIPNVNTNLNIPLDRVIDLPEKNKAIIEKQN
                     YTILAVDDEPINLQVIVNHLKDLGYRILTANNGHMALKMVEEVEPDLILLDIMMPKLS
                     GYDVCKILRTKYPIHALPIIILSAKNQVNDISFGLEQGANDYIHKPFQKKELISRIHN
                     QLSIKQAIEDNNKLISIEKELQMAKKIQADILPAKVPKLQGLDIHAIYLPMQTVGGDL
                     YDFHVVNPFEIGILIADVAGHGVSAAIIMGMVKLAFKMHREFVSDPAVLLQKMNETLY
                     GIIGKGYVTAGCCYINLKEKEIVYSSAGHPALLFLDGKTNLLKELQPKGAILGAFPKI
                     VCRNLSSPIFSGDKILLVTDGILEVRRVNEDGLFYGEEKFYKYLEQKRSLRGQEFLHT
                     IIEDVKSYKGIEDETLGFDDDISIVTIDIL"
     gene            complement(7248..7850)
                     /locus_tag="PLF66_05870"
     CDS             complement(7248..7850)
                     /locus_tag="PLF66_05870"
                     /inference="COORDINATES: protein motif:HMM:NF019235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1564 family protein"
                     /protein_id="HMZ63603.1"
                     /translation="MNDENQQNTYYDWKDIYNDLYQNSQPTPYLNGVSISNRKISFPR
                     EDYIYSEFSSTLLIPDRYYQLFRSKCSSHGGTRQYISYLLSKYQIFIANGLVPGYSNL
                     TTKYQEKGQNLHKISFRPNPQDWAELKLYRVSFGMSISAFLVYLVIADSVDYAEATSY
                     YLAVVGIPETPNFDLSTKIFLTKNRSSYTILFQYRKSHYH"
     gene            7967..8590
                     /locus_tag="PLF66_05875"
     CDS             7967..8590
                     /locus_tag="PLF66_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015676291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HMZ63604.1"
                     /translation="MKRGLFVIFFYISLALALTDCFGNDFSQIKEGDLIFQESTSEQA
                     KAIQLATHSRYTHAGIILKVKGDWKVLEAVQPVKYTKLESFINRGVKRHYVVKRLSNY
                     DQLVNESILMKIKVVGNRYLGKKYDIYFEWNDDRIYCTELIWKIYKKTLNIEIGKLEK
                     LSSFDLSHPYVKNLMKKRYGENIPYDELVISPESMFQSNQLVTVISN"
     gene            complement(8670..9404)
                     /locus_tag="PLF66_05880"
     CDS             complement(8670..9404)
                     /locus_tag="PLF66_05880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63605.1"
                     /translation="MKILFLVLFLFQCRAHLLNRTIGYKEFPMNREGKPIVYLDEPTI
                     YSPMVTIEDSPEIQPKYKLILKEAIRVSILNSGRFSEVKWKDSMLENPEDIYLIEILF
                     SVSELARVGSYPYNQNFFFFYIPYSENEKKNEKIFWPTFIPGFWPASGYFPLLAKSGT
                     IRTNIDCKIKLGKKDPIHLSVDKTEDYNLLFYGVYRTREVEDKAVLSIEKALDDLSLQ
                     LADLELESNKTDVEPIKKSKKSKPRR"
     gene            complement(9404..10237)
                     /locus_tag="PLF66_05885"
     CDS             complement(9404..10237)
                     /locus_tag="PLF66_05885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63606.1"
                     /translation="MIKYFLFLLLLTPILSYADNTNQPLPKEIIGNQKLPEEDLIPYG
                     QEKQRLKYKYNIVTGLPSGLSKFALFSLGYNINGYFSVGISQNQNRQTSEDYSYYDGT
                     KTLSKGVYTEAGSVPSQDYGNTQLIPKMGTNIFLHYYPLIKLDLPIYIPVYLGRSQGS
                     TLINGSENGIFNPAYQMDKPSYPLPILHMSVDAKPALYYGIGLGLKYILPSGVIAGVE
                     FGMVNLYQIQYNYNIQVSLLNKNSLSLSDFLFLQEQLKNAIQPSEKVKLNYGIILGVS
                     F"
     gene            complement(10234..11214)
                     /locus_tag="PLF66_05890"
     CDS             complement(10234..11214)
                     /locus_tag="PLF66_05890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63607.1"
                     /translation="MKNFLIYLFILSLFCFSWNLNADGEKKIKVYPLLAGQGISKELA
                     TKESDKILRALQGLGLQIISEQPDKKYPDTIPSFKKLSSETKSLGGDYFIWGSAKAIG
                     DGSYRILYIVDANTSKAVNEIALECLDLSCDLEEMKAFQEKVKIFLESNSISAKETGI
                     PLIQDNLSDAERKERNLDKAKRSFYFPGLGQAAFKKDTKAFLFAVSFLFTLWIGRDQN
                     IYSHQISQQSRDFYAGAAIANYLNLPLPVFLLGIYKGSALENDSREYGYSSHSWYFIA
                     ASIYVFNIVDAFYISSDTRFQFQISPSTSKHPYEKNYSGFSFSFKKEFDL"
     gene            complement(11336..13354)
                     /locus_tag="PLF66_05895"
     CDS             complement(11336..13354)
                     /locus_tag="PLF66_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020766466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HMZ63608.1"
                     /translation="MTEKEASVGRWHKEFFENIHNFVKSGLTEEEARQLLEKFLYLSS
                     TTPMPPVMNIFKNPDELEKVGVYTQGEEKARNFMMQFFSPIMKNFKVEGQENLEIVEK
                     LIGKFPITLISNHLSHLDAPGIFQLFYSSGPLGRQIAEQMVFIAGRLAFEPDFTRLGL
                     YMFGTLLVCSKKDMGDNPSLSDLMTKINMRAFRQSQKLQGDGKIICIFPEGTRSRDGR
                     LMPFVDTVYHYVANKVVLPISLEGTDKILPTSGFLFNPANGKLVIGKPVIVGDLHGKH
                     KLPVELDEIKFPKVGDKKQFLIDNLALMVGSNLSRHKHGTYRNLYNGDGIAKHKNILI
                     QTPKAPEENIVVIGSSNASVAIAAILSNKNVNVTIYHPDKEYCKNSNQERRDVEHYPL
                     YKLPPNIEFSADDSVIKDATLFIQGTNPWDFDKVYPDILDKLKKNKSPIVNVIKGLTN
                     SPRGLILRDLEEVYGIERFRLAVAAGASYPEQIIERKISGLEIAARDPEIIPKLVKLF
                     TTGYIFSRPALISTDVAGVQLGGALKNIYALCMGLVEGYFKEELGGNVDNTLFHLSNR
                     FFKEMVSIGIKLGGQEQTFNGLSGLTDFMLACFGSDSRDRRYGYELVTGNKPSKMSNG
                     YYGLKAIPNLVELNIEKYPILTSAYNVVIGNANINEAIQLLEARLSRV"
     gene            13525..14529
                     /locus_tag="PLF66_05900"
     CDS             13525..14529
                     /locus_tag="PLF66_05900"
                     /inference="COORDINATES: protein motif:HMM:NF024785.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HMZ63609.1"
                     /translation="MRNDRNTSSELKWIPDGFHFLDHEDSLRRKKILDQIYEFFTSRG
                     YLEVTPPSFDFTNSFLSHVANSEEARILKSRDLSGREISPSIDLTLQVVKGLAGFGVK
                     KGNQKVFYTGRIVKDNFKKNADRREILQAGAEIIGHSNANTFKMLLTHISELVPVLGL
                     KEKITLVLGNTAIYSNIVEYLEFSEADKNTLSNLLYHKEEKSIRDFLDLRVKKKEIRD
                     LILKLILSFELKEIKSDLISISKKYKIKLEPLIDETEEIFKYANHFKNLDLCMDYSLI
                     RDLDYYTGFVFHGYYDRISYPVVTGGAYDHLFEKFSGEEKRACGFAVNIDIIEEINRR
                     "
     gene            14545..15816
                     /locus_tag="PLF66_05905"
     CDS             14545..15816
                     /locus_tag="PLF66_05905"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004783637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HMZ63610.1"
                     /translation="MPADLVIGAQWGDEGKAKVIDYLSKEIDIIARYQGGANAGHTVV
                     VHGKKYVFHLVPSGVIYPNTICVIGNGVVLDTDYLMKECDELCKEDFDVYNKLYISDA
                     CHVILPLHGKIDSHRERNSTPGTKIGTTGKGIGICYADKMMRIGIRTGDVLDEETLKD
                     KLKHFLAKKNEELVKLYGEEPVQFEPIFDSLKKFADKFKGKIINTAYFLNEELRRGKR
                     VLLEGAQGVGLDIDFGTYPFVTSSNPTTGGALTGSGIGFQHLGKIYGISKAYATRVGE
                     GPFPTELFGEDAEKMRTIGHEFGSTTGRPRRCGWFDLEMIKHSIRICGINQLVITKID
                     VLSTYSKIPVGVGYELNGKKLDYMPSCGMEKVKVIYEELPGWNTDITGINRFEDLPKN
                     CQSYLKYLNDKIGVPISIISTGPDRKDTIVL"
     gene            16802..19075
                     /locus_tag="PLF66_05910"
     CDS             16802..19075
                     /locus_tag="PLF66_05910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63611.1"
                     /translation="MNVAIKAVNNKKEINPNLILEAVKNNKPITIKTYYLTESTEEYI
                     RSIIQLVMGRVGKTENIETCYNSVRGLVVNATRANIKRILFKEMGLDITNPTEYLIGM
                     EKIEPKLTEIHFPKYKAELTRQKLIVETTFNFNPDMFSVKVKNNFILLPHEYQVYGKS
                     SSNDKSSINGSVVRVREVISNGQVAQTPSKVVSLNQYKIDQQVLSHVMNEDTKETIFK
                     LDIVLKDKNTFNTENFGQELNALFTSYKEKFDKVFKENVEGLDTRDYLTVSKDLAKSL
                     TGLRGRGDFLNLYQRIQNLDSQLYKEQQQLLANYGEHVFTAIQDKLSKAKAESNEYIS
                     QIQRIGKEVFEINKKQIDEFAQQKDFEIEKQIKDSIDRHKKKLEEFNDSLDKRIENQS
                     MIIKSQLSQQMEDMNRSSADLLKSAKNEIAKVKEELKADLESEIHIAELMKSDLFQDI
                     KADKEVLERTLATVSEEIQKIEKFHTNKDVMDNLIAQSEEAFWKMSSNIEVIKAKEEN
                     INEYMNNINLLETAIRNTEREIRELNDEKTEYLNEKGKMVQTIDTRMKQMEKFGEELK
                     QKLVEINSFERKINVISGALSEQVKKTKNVDETLAKFSKEVAAFETKKNELGRFITEV
                     DQKVALINSKTTDIKLLEAKFNHIERLMIDLSARHKQIATMEARLDDVKGNMEKLLSQ
                     AEIKMNQMSSVINNAEQKSSSKPRAKKQASPKFSSVLKDLKESILNLKKKKLSPQEIA
                     SALDIDEEMVSMILAMQ"
     gene            complement(19160..20605)
                     /locus_tag="PLF66_05915"
     CDS             complement(19160..20605)
                     /locus_tag="PLF66_05915"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HMZ63612.1"
                     /translation="MFDWIVIGAGYGGVAAASLLQKRGFATCILEAHSLIGGCASYFK
                     RKDFLFDVGATTFSAILPNQPLGKLFHELEITPDLYKVDPGMIIKMNDQKITRFSDLD
                     KWVNEVNKHFPDLKMNQFWNKINSLEKLAWEFIDQNSNLPPRTVSDLISLIRFKNFNK
                     IVLLKELFRSVLDELKSLQLQRDDFIQFLNEQLLITTQNRVDLSPMLTSAMGLSYPKE
                     TYYPIGGMYKPAQLLLNHFKESKGEIFLKEEVIQISFNKDCYLVRTKKGNIYKARGII
                     SNLTTADMAKVTEGKIQSYFKKESSKLKNASGAFTIYFAVENNEILDSLYYQIHFRKK
                     IPFIEAGAFFVSFSKEGDLEKAPIGYRTVTISTHTNPVEWSDLSKDLYKEKKEFVMKE
                     ILGEFDFHFPKMKQKIFPLGGSPKTFADYTGRHQGFVGGIPHSIQNNLIFMTPNVTPF
                     QNLYMVGDTVFPGQGIPAVVLGAINVVNRIC"
     gene            20685..22154
                     /locus_tag="PLF66_05920"
     CDS             20685..22154
                     /locus_tag="PLF66_05920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63613.1"
                     /translation="MPDIKYICLSDMHFGAQNSLLTKLTPDGLKPNFLEPSPVLTSLI
                     DQVKLLLNENTNKKEKPVLILLGDILELALCETQEAGMVFDRFIDLILPKDKELFSAI
                     YYIPGNHDHHLWEIARESQYIRYIQRKADNSILPTMWHTTNIYMENHSYPVTCDFMNA
                     IIQNHKHLQKFEVQVAYPNFGILDKSGQKSVLFHHGHFLEDIYTLMSSIKTYIFPDQK
                     IPDDIWDIEKENFAWIDFFWSTMGRSGQVGKGVELVYDKMTDENKFSELLHNISVKIT
                     QNIKKSESAEATTFFRRMSKFFLRFIPGRKIEKIIYAFLKITLGTIAKHERTDSGGSL
                     SDEMRDKLVFYLNQTLKRQLNREKKENPPKEIQFVFGHTHKPFSEVMANLDYPQKVQV
                     VNTGGWVIDTVDRKSTYGGAVILIDENLNIAPLQMYRESYESGKPKVAILENTINNEF
                     TKKINEIIEREKNSFDSFSKIVDKEIDLRITNLKAKINR"
     gene            22171..23886
                     /locus_tag="PLF66_05925"
     CDS             22171..23886
                     /locus_tag="PLF66_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008595037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HMZ63614.1"
                     /translation="MYEAVVIGSGFGGAINACRLSKKWPNGKVLVLERGKRYPMYSFA
                     RTPHEMSKNFWNIPSETKGGNPEDEEVHGLFDIRNFHKMDAVISAGLGGGSLIYANVF
                     LEPPDWVFNERWPETCKKEKLLPYYKVSKEVLGARPIPTNDDPRRKIRRTQRFQEFAK
                     EMGHNSQLLDLNVFFGNDFKNPTPIGIQEKNKYGAIQTSCTYCGECDVGCNTHSKNTL
                     DLNYLFVAENVHKAEIRTEQLVKKIVPLDASGKEDPNADGKNGYRIYFVNLTKSRGTD
                     RLESVDSKRIIISAGSLGSTELLLKCRDEYKTLPNLSSKLGEYFSGNGDFLSFVIRGK
                     EESNPNYGPVITQAIDFNLYKEPDKKRAFVLEDASYPAFAAWAANTLPSTSIWKNLVA
                     AIKDIWFKITREGSAYGRSGYIFNEMLANDVSQNSSVLLFMGVDNSNGKMFLKDGELE
                     INWTQEESMPLYDKILDISKKFYEWAKAKFWFPLFNWNAISKDNVTVHALGGCILADS
                     PDKGVTSADRKTFGQVFGYTNLYVADGAIVPTAVGANPIATISALSEMVAEGITGIKP
                     DSSLK"
     gene            23940..25289
                     /locus_tag="PLF66_05930"
     CDS             23940..25289
                     /locus_tag="PLF66_05930"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HMZ63615.1"
                     /translation="MSRRKVPGPPKSHFIFGNLNEFQKNPPEYIRKVTKEYGNISAID
                     LAGFLCIIVNEPEVIKDIMMTNQKQFVKAHAFRIFKKLLGEGLLTSEGEFHKRQKRLA
                     QPAFFKQRIHSYGPVMVDYINRIQSTWKDGETIDLHTQMTDLTLGVVTKTLFNTEVGT
                     AESRAIAQSITEALEIFDEVLASPFADIILKIPFLPITMRLKKAIKKLDKILYQIIDE
                     HRKHGGDQGDFISMMMLAKDEEGTGSMSNKQIRDEAITIFLAGHETTANALCWTFYLL
                     AKNPEKRKKFLAEIDSVLGGRKPTVEDVPKLTYTLMVFSEALRMYPPVWWTGRENLEE
                     YSIGDYKFKKGTIFVISQYAMHHDERYYSDPYTFRPERWLPEEADTRPKFAFFPFGAG
                     IRMCIGNQFAEQEAILALASLSQDWLLHIEEEKQVLSTMLTLRPKDGMKIKLEKRKR"
     gene            complement(25286..25717)
                     /locus_tag="PLF66_05935"
     CDS             complement(25286..25717)
                     /locus_tag="PLF66_05935"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HMZ63616.1"
                     /translation="MSKSIQFTTSVEINANVEKIWNLFMDTSKYSEWNPFIKRVYGEI
                     APEKTILEFAYFYNHLYLPILMKIIEFENKKKITWVGNFPGFSDHHVYEFQEINNSKT
                     VFSHAATFSGFFAISSKKHIETDVKRVHEEMNLALKKRAEN"
     gene            complement(25728..31175)
                     /locus_tag="PLF66_05940"
     CDS             complement(25728..31175)
                     /locus_tag="PLF66_05940"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HMZ63617.1"
                     /translation="MIQIGGFKVEEQIYESVNSRIFRGKRTSDNTPVIIKKISQDFPS
                     PEEIKQFKQEYEVLSKLKTDGVVKAYALERDGNSVVLVLEDRGAESLRKIFSGTKLEL
                     IQFLEIGIQCAQVLGQIHAARIIHKDINPSNITFNVQDGKIQIIDFGIATHLSKENPV
                     IKNPSSLEGTLPYISPEQTGRMNRSLDYRTDYYSLGVSFYELLTNEMPFYSDDPLEFI
                     HAIITQEPKQVSSINPNVPEQVSRIIAKLLAKNAEDRYQSSIGIVKDLEQCLEQVKIN
                     SKIDLFPLGQYDVSQEFNIPQKLYGREKEVEWLLTHFEEYLESFRRKGLSATRQIPIM
                     LVSGYSGIGKSVLVQELFKSITKQRGYYIAGKFDQLQKNVPYFAILKAFQELVRQLLS
                     EPEEALKSWKQKIIRELGNNGAVITDVIKELELVIGEQPEVPELGPSETQNRFFETFQ
                     RFISVFAKPEHPLAIFLDDLQWADTASLKLLESLSLGEQVCIFIIGAYRDNEVNDSHP
                     LKITLKEIKDRFKDEYSDFVVEIPEIKLNPLSKENIEELISDTLRMERANVRDLASLI
                     HEKTGGNPFFTNEFLKSLYNEEYLVFDPNFDSKSGYTPWKWDLQRILGLNISDNVVEL
                     MTSKIQKLSNHVQYYLRIASCIGNRFSLEGIALITNEETKPILTNLTEAIQSGLVQPI
                     NENYKFIDLEIKYDGNAEFKFLHDRIQQAAYTLIPVEEKSKVHWEIGNFLLSKASEEE
                     LDDHLFDIVNHINQGLVHIKEEDKIKIANLNLKATSKAKLSAAYETAISYGKTGLGLL
                     GLPTSKEAAANSDKSFWTKNYDLVFSLYFEIAEASYINTNYEECEELVEIILPHCKDI
                     LATTKISEIRIRSLIAQNKLIEALDTSFPILDQLGFKFPRRPNKLHVAKGLLGAIVKF
                     SDKKLLELAKLPKLDDPKLYAGLNLLATISASAYLAMPDLNPLILFQALDKTFKKGVS
                     DISPILLAGYIIILCAILGKLDVGYKLCQAVLIVYEKNPNPKFYASILVMMNLFGVHW
                     KESMGQTIKNFMEAYKIALQTGDLTYAGYAIFNYIRNSFYSGKELSQLNKEAAVYSKK
                     IKSMRQESALWLSASFCQITDNLLGKVEDPSILSGEFFDREEDLKRLKEQKDNSSIIN
                     FYLQELILSYLFGKYEVGIKSAKEYRKFIDSDISTINVPIFHFYEGLNLSNLIKKPSG
                     LSYWKFKSNLNKMKFYHDKAPLNHEHRYYLLLAEQGRLENNSEKAELNYDKALELAKA
                     KGFPNDEAICAELAGKYYLSRGRRVIATHYLMNAYNAYNIWGATAKLRQMETVYQGIQ
                     LKRVEAKGATTFAHTTISNSSTTVASTSVTKNSNEAFDLASVMKAAQSMSGEIVLDKL
                     LRNLMSTLLQNAGADRGILILESKKKFFAEAIYETGKSPQILESIAIDGSTLICPTSM
                     IYFVVRSKENVVIDDAIVDTKYGTDAYIQEAKPRSVMCTPLMTQGKVVGVLYLENKVS
                     TGAFTKERLRILNLLSSQAAISIENARLYANMSELNSAYQRFVPEEFLKFLNRESIVD
                     VKLGDQIRKEMSVLFSDIRSFTELSEKMTPEENFNFINSYLKRMGPSIRENNGFIDKY
                     IGDAIMALFPRNADDAIKASIDMMKGITLYNQHRKNQGYGPISIGIGIHTGELMLGTV
                     GEDKRMDTTVISDAVNLSSRLESMTKQYGVGIIISEDTLNKMQAKEKYKFRLLDTVVV
                     KGKTLPVVVYEVLDYYPEDILKAKLASKEMYEKAVQLFYAGSFVEAKQLFDQVLLLNK
                     EDKATQVFLERVDGLMKNLDQWKGYSKLTEK"
     gene            31479..33254
                     /locus_tag="PLF66_05945"
     CDS             31479..33254
                     /locus_tag="PLF66_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010570851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HMZ63618.1"
                     /translation="MPASKEPVNLQFTEEMKGYLTFSDKQLDYKESFDSGKKEDNYFM
                     FHLTIIIPDVDFFVNDPKETGVAEGYVESPKMGGRFTVEKGVFNCFVDVDAPVKNTKN
                     MFYRLFFKDNNGKPLTMTGHKVVRNDAGIDIWKDTTTLYTRIYEGHVEEKAEKTSKLV
                     ATGILEIYVKDFAKQMTTIKSNGKTFVERERAVMQFGKLFLGNLWDVYRPYIKKGEPE
                     IWNQREIPMFTFEGVKEGSITNHPITTGDKLGISLTRFKKKECDDVVILSHGLTTSTD
                     MYIMPEHNNLVNYLHENGFSDVWSLDWRGSMRHSYNLFPHRFNLDDVALYDIPAAIAE
                     IRKEIGPKKRIHAIVHCVGSITFMMSLFGKKIDGISSVISNSVSLNPRVATWSKIKLA
                     VAPFAIEYLLRFPNVNPRFSYSPGPALGKGLAKIVSLFHRECNNPACHMLSMMWGTGF
                     PACYEHSQLSPITHDRVGDLFGATSMNYHRHIRRMVSKGNAVKYQIENPRYNDLPNNY
                     LDYASEINTPILFMTGDKNRVFNDSNIVTHDTLKKIKPDNKNELFIAKGYGHQDTLMG
                     KNVDKDIFPRFLEFLNKHKTPKGWF"
     gene            33316..34173
                     /locus_tag="PLF66_05950"
     CDS             33316..34173
                     /locus_tag="PLF66_05950"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HMZ63619.1"
                     /translation="MKIAIIGDVHGFWNEFDVEYFNQSDYDFILFTGDLRSYTEKESV
                     VAERISFLEKQAFVIPGNTDTSNSFQAAGEIFRNQFLIQNFFLGQESRLDKMKAALGK
                     LRLVGYEVHTLSKEKSIDLIVLRPFAMGKELSYAPILKKRYGIRSMEDSKERIKTLID
                     SSLEENLLFLGHNGPKGLGEKETSLWSSDFTETPIDWGDEDYLFGIEYAKSRGKRVLA
                     AIAGHMHHRLKKRNILRNWREEKEGTYFINAARVPRIFKIDSNTVHHHIRLEINKGDV
                     SAREMLIHP"
     gene            complement(34175..34516)
                     /locus_tag="PLF66_05955"
     CDS             complement(34175..34516)
                     /locus_tag="PLF66_05955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63620.1"
                     /translation="MTYFLATTSEPLTILVDIIFITAISSLIAWYFYFYLKKEIIGGF
                     IGAIVISALGSILLLASLQSFIRDIFMWLMSPKLGTIQLSNVNLIVVFIGAYLSLYLL
                     IKINYNRKRKE"
     gene            complement(34581..36860)
                     /locus_tag="PLF66_05960"
     CDS             complement(34581..36860)
                     /locus_tag="PLF66_05960"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HMZ63621.1"
                     /translation="MFKRLYIKLTLRLETFTHIVPLPFAIYFSVVTGTGSYLSKDEMM
                     VVALAGSLNATALFILGCVWRFFYLKKIFKQIKNFYTEKNISKEDKVKIKLKLLNYPF
                     QEAKIIVLRWLTGIIGSHLFVILAAGFRPGLHLTAPFLFLMITPISYTAYYFISENVI
                     GAIFKLKKVKEIEIGINQIPQFNSFQRIILSMISITVMPVIVLGYMLYGMKIGLVKLE
                     DPGLHLTIMSLLFPVPVIIVGYVVAKTFRNGIMGINEHLNQLGKGNFSIVSMPTSSDD
                     FGLQAYNLNEIIKQLNSLYREISDLNINLENKVKERTVELNYSLKNVETMKIQQDGDY
                     YLTSLLLRPLGQNLAESNNFKFSFFVKQKKTFEFRGKENEIGGDICVSHTIELRGRTF
                     HVFLNGDAMGKSIQGAGGSLILGSVFQSIVERTLLSATTSEQSPENWLKQAFIEMHRV
                     FESFSGSMLVSIVVGLVDELNGFVYYINAEHPPSILFRDNKAEYIEGEPLFRKLGTLQ
                     INTGVFIQTFQMYPGDVLLIGSDGRDDLRISNETISGMNSNPLRILKIIEETDCDLPQ
                     IYKKLLTYGEVTDDLSLLKIEYTKEINLDRKTLPDIYSIPRIVEKLLLDKKGEEAIQF
                     VISKIEEYPDEYSLLKILSNVYFRLDNLQMATITGGRYIDFCPGDLDFLHKLSTFYKM
                     IGDFENALEIGERLRMRDPQNIKNLHTLADCHSLINNFKRAEEILQYCMKLEPNQKET
                     MNLKELIQKRKSDYIKSFR"
     gene            complement(36903..37544)
                     /locus_tag="PLF66_05965"
     CDS             complement(36903..37544)
                     /locus_tag="PLF66_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010571760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HMZ63622.1"
                     /translation="MKIVKAEPKKKARRIRNSLNKAEILSAALHIIQKDGVEFLSMRS
                     IAKLLNCSVASPYAHFKSQEEIIQTLLFNGEQKLTSDLKLARASSENPLEQVKAIANT
                     YWQFSVENQGLHKLMFSSGGGLHKKLFPAPSSYRVFLRTIRDGLIKKSGGRPNPHYNS
                     IARTMWAWMYGLLVLQMTEILDRDKLKIDPIEEGISVFSSFFQNTDPLTKKIK"
     gene            37717..38781
                     /locus_tag="PLF66_05970"
     CDS             37717..38781
                     /locus_tag="PLF66_05970"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HMZ63623.1"
                     /translation="MVEALEKEEMVKEPTLSEIKQVVREVCFKPNRRKAGLFLFSGVI
                     QFILSVSFSIYVWEIGVYALFPIAWFVSGFVFTSLFVLGHDCAHESFTGSKMLNSFLG
                     HILFIPTFYPYYAWKYSHAAHHQHTNELYAPSNTVYFDNAWIPMTINEYKVMQKKYPV
                     RAFLYKLTRIVLPIGSFIHNIVYHYFPNKFKPDHRKNVYFSYFVLGFVGVSVAVTLYY
                     ATGSWFGIFHFWIIPALVFQVWMSLYTFLHHTSSEMQFYPKKDWSQYKGQIKSTTNYR
                     MNKLISFLHFHIDCHIPHHLNIKIPSYHLLAAQKDLSNSKYSEDIVEEKFTWKHYFKT
                     IRECNLWDEKNRRYVRFCDV"
     gene            39574..40758
                     /locus_tag="PLF66_05975"
     CDS             39574..40758
                     /locus_tag="PLF66_05975"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HMZ63624.1"
                     /translation="MLKRLEEFLVKRILTYRTGSDHFRVAHNIFSLKFFELNLEKEFL
                     DFNLLNSIFQARIAAFLGLLLYPSFLLVDFILFKREDFEFSVYLRLSITIFPILFSFL
                     YSIFADLENKSTIDWIERIAIFTVLSAQLGHCALLFFTSSSPYYDLAVTTIILFFIFG
                     ITKIRFIFNVYLLLLTDLLFGVSLYVSQKCNNEVFFAILFILTSFSICALLVGYIAEY
                     QNRRKFILLKKLNEEKQNNIETKNELESVNLSEILQFQQTNLDYNSIEGIKDFQYEGE
                     IELDRSRMRRVIGNLIQNSIEVMYDGRKPYYVKVFSREEGDTCVIGVEDNGPGLPESL
                     QKKIFEAFATEGKAKGTGLGLFMSKWIVESHGGSLTYQTTSGEGTTFYIKIPKKNKKE
                     KQ"
     gene            40755..41117
                     /locus_tag="PLF66_05980"
     CDS             40755..41117
                     /locus_tag="PLF66_05980"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HMZ63625.1"
                     /translation="MKKILIVDDQPSIAMVLGDILEEGDFQIVNAVNGEVGIAKAKSE
                     KPDLIIMDIMMPVKDGITAIRELRAMDEFKELPIFILSAKGGAHDENLVQELKISGFI
                     HKPFSPGVILEEIQKALS"
     gene            41174..43138
                     /locus_tag="PLF66_05985"
     CDS             41174..43138
                     /locus_tag="PLF66_05985"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HMZ63626.1"
                     /translation="MKIPKVEKGVLDLSSLESVDAKDFISSTPISIDGEWEFYWKEFY
                     APSDFLDPNKQEEIEKNREYLQSNSRWNGSISNAKKETAKGEGFATYRMRLIHTPKEI
                     THLKLSNVYTSYRIYINGVLLHESGLAGKDKESSKAYVRDKIVKLPDSKTGQFEILFH
                     VSNYFHRLGGLVYPMFLQSESFIYEEQKKEFALALFFTGSFLLMSIYHFGLYLLRTKD
                     LSPLYFGIITLLLGIRPLVTENFILDYFPFLEYGLVKKIEYFTIYLTAPVIGLFFQSV
                     FYKTMNRKIIYGFVFISSLFCFVVLFFPVRIFSETLISFEILVLMCSCFILYSILKAA
                     LQNEIGSKTLLIGFGIFFITVVNDILHQQGIIYTGNLVPFGFYIFIVSQAYLLSVRFS
                     NAYREEERAKEEAERQKKYLADAKKEIENLSKTKDEFLANLSHEIKTPLSIVYAYSQM
                     LPASKENPKKIEKYADQIYSNASKLNDYVSDLLLVTDIESNLELQKRETDFNQILKES
                     MDTYRDLVEQKSIQVEYQENESIYVNCDELLLKKSIQAVYKNAIVYNRHHGKIKIFVE
                     KKDNKLELQIEDTGIGIAKEFQERIFEKFFRIDSSLNYETSGVGIGLFLAKKILEMHL
                     GSIHVESELGVGTKFTIQIPILNTVGVSNV"
     gene            43131..47474
                     /locus_tag="PLF66_05990"
     CDS             43131..47474
                     /locus_tag="PLF66_05990"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HMZ63627.1"
                     /translation="MYKWLFLFFLIFIFDCKNPNAISPPKAIKGELDLSNWDFHSPDT
                     DGIVKLDGEWEFYWNEFLDPTLNASDPIGNDSIVGRHENKSFIQVPGEWNKYGYPEEG
                     FVSYKLKIRLPQGKRKFSLYLRNISSNYNMYINGELVTAVGKVGKSEEDSSPYMAYKK
                     ILIPETISDSNKLEILFHVSNFHQSGSGIWHPLSIGTYENIEEIYKKKIALDLVVIGF
                     LGMMGLYHLGLYSIRKKDRSPFFLGLFCLLIVIRVLTINERLIFELFPSLSFLFVRKI
                     EYLTFYFGSYLFFQFVYSLYSEGFIKSIFYIVNEIFLLATLTVLFFPMKIFMFLLPIL
                     QIIVLIAIIQVLHATIVAIRLKKMGAIISLLGFILFAVAIINDMLKGQGIISTPSTVS
                     YGLLVFTFFQAIVLSRRFADGMEKSEKYSEELQKKTDRLEETTVELKQLSDNLETKVA
                     ERTDALEKTNKEIEKLNRITKKVNSVSDLTDILTVILFYLEKEYKFNEFWMLLKEEEE
                     NKLSTFAYVSSIASSDTITKMKNFQINLSESSLVIEAYNLKKVEKFLIQEKNLSTLDK
                     TWIEINAFTYFLSLPFVVFEEVVGILLVHHPSIKEINIEQIPKLQSFTDQIAGAISNS
                     RLFKKAQLAKEQAEQEKGIALIAQLETEKQKKEMAELSELIKSLNEELDIQKIMNKVY
                     EFLQRNFGIQYYALYTVNPEQTYMLPFNIKFPEFVTEEEQNKILSMVIPINQDLGSHA
                     FALRFKKPFYIKSDQVIKRFDKNVLEEQEIIRITQINSMLIIPLILDNKAVGFLDLSN
                     SKNPLNLSKEDITRLSILGEQLAGIVHGSNLFKQVQEEKEKAIVAQRELVLVNKEIEK
                     LNKIMKKINSVSDLTDILTFILFHLEKDFKFNEFWMLLKQEEKEELYTFSFVSPFATP
                     EKILSLENIKIPMNAPSLVWDAYQSKKINQVFLEEKNLSELDRKLKDIHNFSYYLSLP
                     MVVYDEVTAILMVHNQSISSFTNTQIEKLQNFTNQVAGAISNSKLFKQAQIAKEQAEQ
                     ARGEIEKLNEFTKKINENFNLDEILDLVGKYILENFNIGHYLLWKMDANQSELFPYKG
                     TFHESIQKETINQFMKLKIPMNENSGVHGAACRNKRYFFVRGMGRKKSESEAENKIQE
                     LLQFKSLLIVPLLIQNEVIGTMDFSHYSSEMNLSKEEISKICVFCEQIAGVLHGASIL
                     NQIEAERQKSEKLLLNILPKDVAKELKEKGYAEPILFENVSVMFTDFKGFTQIAEKLS
                     PQELVKDLDACFVQFDKISERFNLEKLKTIGDSYMCAGGIPRRNTTHAIDCVLAALEI
                     QSFMNMMKELKAAQGFPYWELRLGIHSGPLIAGVIGEKKFAYDVWGDTVNSASRMESS
                     GTPGKINISGTTYEIVKEFFDCEYRGEVAAKNKGVVQMYYVNGLKRQYSKDEEGKIPN
                     GKFWELYSGGL"
     gene            47803..53091
                     /locus_tag="PLF66_05995"
     CDS             47803..53091
                     /locus_tag="PLF66_05995"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional serine/threonine-protein
                     kinase/ATP-binding protein/SpoIIE family protein
                     phosphatase"
                     /protein_id="HMZ63628.1"
                     /translation="MDQIEFAFENKEILHKNASIEVSRVKIRGQENSVIVKRIISPSP
                     EAYEYYRLKNEFKILSKFPLSKSVKVIRLVNERTLVLEDTGGISLKTYLVNTNLTIVE
                     FLDLAIRMADALDEVHKKNIIHKDIKPSNIIIHPITKEIRFTDFGIATLFAKEYIPSL
                     GKLEGSIEYVSPEQTGRTGTALDYRTDLYSLGITFYEILTGELPFKNKDQNQILHSHI
                     AQQPKPLSEIYPSIPNSISDIVSKLISKSPEARYQSAEGLKLDLLELASAIGEGRIPS
                     GVEGSGIEGKFFAGKNDFSEKFSIPQKLYGRDREIQSLKNSFLNLFSKEHRTATLQLI
                     NGYSGIGKTSLVQELNQPVVEKNGIFISGKFEQYQKNVPFFAVVQAFRELTRQLLAKD
                     KTELLNWKNELKEALGNIGQVILDVVPEVELFLGKQPPIPDVPPDQAFNRFKYAFSQF
                     VRVFAKEKHPLVLFLDDLQWADMASLSLLKNLLTDTDIIYFFVVGAYRDNEVDSSHSL
                     TFFLHDLEKNGLKIENLQLGNLDKDSVLSLVSDSLLSKKEDSLELTELLLEKTDGNPF
                     FVRQFLQSLYEENLINISERKWKWNISEIRNKSVSDNVLDLMLQKIEKLTEPSQLLLG
                     YASCIGNQFDLKVLSFLLQKTTPDVISILEECIKAGLIIPLSDSYKWIESDSSDNCSF
                     RFLHDRVQQASYRLVKEEEKAKIHFIIGKILLEHTAKEELQEKLFDIVDQLNYGLDLI
                     QTEEEKIQIVELNIQAGKQAKASVAYNGALGYFKNAEKLFSKDAWDTNYSLIYQCVTE
                     LGETEFLCGNFEEADITFEIGLANAKSRLDKAGIYNLKVVLYTGQGKYNQAIETGLLG
                     LELYEFKTPENFGVTNVFKEIKAVKALIGDNPVNRIKTLPELVDKDKLMHLHLLINLV
                     DSTYFTDKILNGYIVLQSIQFSLINGVSLAGYISFVSFGNLLCANSDYQFGFEIGKYI
                     NEITSSNNLTLIKSKSDNIFCAHINHWKRHIKTGFEFQLEGFKAGIETGDLSYAGYNV
                     AWYLWYRFFHGANLEGIQKENLKFLEFALKVKNKNLELLIRIIVQTIQILREEDIKEK
                     DSNLFEECRKSEMKIPLFGYLLLDLELNYHLERFETSPSIIKQSFEHQDSVFGLMFYL
                     EFHFYYALTLIAMHLEAGTEQAQNEKAILQEIKLWKRWAEACPENYLHKSLLIQAEWA
                     RVKGEVVSAMDLYEEAIESAKKNDYILNHALASKLAGKFYLSLNKPKFAYLFFMDAFF
                     AYKNQGADLYARDLEEKYLKKHFQYMGKERRQQKVSDSTSTTLASIDLNSLLKASRVI
                     SSEVKLELLLKNLLYIIIENAGAERGVLLLEEKGDYLIFAESKANSETNLEKRQQDNS
                     HLPLSLIQLVLHTKEYVILHQAKEDKQFSKDVYIAKNPILSVACFPILNQNQLIAILY
                     LENNLLPNAFTPERVEILNVLCSQAAVSIQNAEVYENLEEIVRVRTQELKTLFEEVKE
                     LKVAQDADYYLTSVLLSPLQVNNNTAETVHTEFIVEQKKKVKYRALREECEIGGDLCI
                     TDTIELNGRTYTVFLNGDAMGKSMQGAGGALIAGVVFKSGITRTKNENTNSLTPEEWI
                     QERYWDLQKVFLSFDGIMLVSICMGLIDTEEGTLYFLNAEHQAPVLYKDGVASYVEHP
                     IQLAKIGSILNEKKFFLDVMKLTSGDQIFIGSDGKDDIKIIDPETGEETIQSDPDQFL
                     QIVNQTKGDLKEMVSEIKKSGTLIDDLSILKISYLP"
     gene            53163..53945
                     /locus_tag="PLF66_06000"
     CDS             53163..53945
                     /locus_tag="PLF66_06000"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HMZ63629.1"
                     /translation="MNPFKVENQELKGGKTAFIGIQTSEQNTVTRDQILELQRILTDL
                     DKDSTVRGIVIHSENEKFFCNGMDAKHISTTPKDRLAEEMGEIIKFFVFMSEIYKPLV
                     AEISGYAMGGGAVLSLACDYRMMLTGKARISFTEVFFGLPLPGVLIEKCKMAVLPQHV
                     NDVIYGTNYKAEEAKEIGFIHEIADNKEDLRKLTVRKLENLYKFPLSAFQKTKESLHY
                     HLSQYADAHMKALDANFQNPVIQKNLMEAMNAFQEKRRPNFS"
     gene            complement(54651..54953)
                     /locus_tag="PLF66_06005"
     CDS             complement(54651..54953)
                     /locus_tag="PLF66_06005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63630.1"
                     /translation="MSRMKLVFITLIVLFSNCENPDYPTGSKTKKKKKHCIRETTLYY
                     LLYSNDYRFSCNNPDYVKGINAKSLDECLSKRNESIIFNLGTVYSLCSSNEEGSDG"
     gene            complement(55121..>55567)
                     /locus_tag="PLF66_06010"
     CDS             complement(55121..>55567)
                     /locus_tag="PLF66_06010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63631.1"
                     /translation="GNKVISQKTILIRRCLIGQVYRSAPNDCQGTGTKDTVWGASQLQ
                     WCPTNDRSCEKDGKADPAISPAAKACATDTFAGKKWKLPSKDDSLGTLLSELSGGFYN
                     TGSNYIAVTWLMESISENKALYARALYNFRDSDKNSAGEVLCKEDI"
BASE COUNT        18603 a         8828 c         9835 g        18301 t
ORIGIN      
        1 tagcggtcac tctttcttat ttacaaccaa agaaagcggc tgacgtttta aaacatttcc
       61 cacaggaaat gcagtccaat gttgccttaa aattagcagc aacttctaag actcatccag
      121 atgccattct acaaattgca aaagttctaa agaaaaaata tgactctagg gacaaatcgg
      181 aattagccca agcaggtggg gcagaatcat tagcaaatat tctaaatttc atggataaaa
      241 acatggaaga gaatatcctg aaagagttaa acgaaaaatc cccagatctt gcctttcaag
      301 ttcgcgacaa gctttataca tttgaagata tcttaaatct agatgctaga gaaatgagaa
      361 ctcttgtaaa tcgtctcggc ggcaacgagt tgttaatcgt tgctttaaga ggtgcgggtg
      421 atgaaattaa gagacatttc tttacagcta tgtcccaaaa cagagccgcg gatattgtag
      481 aagaaatgga tagtagaggc aaaacaaccc ttcgtgaaat tacaaatgca agaaatgata
      541 tacttagaat tgctcgtgaa ctagaagaag aaggcattat aattttaaag aaacgaaaag
      601 atgaatttat atgatttaat gaataaaaaa tctaattcaa caatagattt acttctaaaa
      661 atttctaatt tctccacaat cagattctta tttatatttt tgatcctacc tttttttctt
      721 tcagctgaat caatcgcaga attaaataaa aaaggaatac tcgctcttca aaagaaagat
      781 tataaaaaag cagaggagat atttaaaaag tctctttcta tttctcctaa tgatgcaaaa
      841 gcaaactata atttagcctg cacatatgca ctcatggaag aacaatgtga gccaagtgaa
      901 aatggcaatc ctttgacgtt attagaaaaa gccgttctag gagatgttta ctataaaaca
      961 aaaatgctaa aagactctga tttaaaatct cttagaaata atataaagtt ttataaattg
     1021 gcgggtctta caaagaaaca aatattgacc aagattattt ggtatggacc taagccagga
     1081 gtagcaaata ttgataaaat ggtttttact gattcaggaa aattttatta ttcagtgttt
     1141 aattcttctg gagaaggtaa ccctggctat actgaataca atggaagata tgtatggatc
     1201 aaggaagata tccagattca attcgatgag agtccattcg attcaaaaaa gaaaatcttt
     1261 aatgtaaaat taaaagaaaa ctcaattcag atagaaggat ttgaccatga gctatctgat
     1321 tttgaagaca gatgttctgc ctgaaaatat ttcgatcaaa caaccttcag aaatatttta
     1381 atttcatcgt agtcaattcg atctagtttt aattttaact tttcacctag aaaaaatgtt
     1441 ttggtaaatc gtttcgcgta gaacgaaaaa tcatttggct gatcgagttc gtattcattt
     1501 gtaaactctg tgtaagaaac aaccccctcc gtaagtattt catctaactc aacaaaaata
     1561 ctgtgcggtt taattcccgt gatagttcca gtaaattctt taagacctgt tgcctctacg
     1621 aaacgacatg cctttagctt taatatatct ctctcggcat ctgctgcacg tctttcttct
     1681 gtagatgtat ggtatcccat tgttttaatt tcctcagatg agtaggcgtt gtcttctttg
     1741 tgaatgagtg cttcgagtgc tctatgacaa acaaggtctg gataacgacg gatcggagaa
     1801 gtgaaatgac aatagtcttt aaatccgagt ccccagtgcc caagtgcttc tccgctatag
     1861 taagcctgca tgaaacttct aagtaagaaa taattgaaaa ttttctctgc tggatgattt
     1921 gctattgcag ataacgcttt tttgatatca tcatattctg ttgaattgag ctgatattta
     1981 aaaccatata attttaagaa ttcatttagg ttttcaattt tatcctcttc cattgactcg
     2041 tggactcgaa atagagtagg aactttgttt ttacgtaaat gttctgctac ttttatatta
     2101 gcagagagca tcatttcttc gattagaatg tgactattga gtctttctct tgttttgatt
     2161 tctacaactt ttcgattgtc tttatctgtt actacatagg tttcttttat atttaaatca
     2221 acacgtccat tttcaatacg aacttttttg agaccattgg caagtcgcat aactttaaag
     2281 agccaatttt ttgcatcacc ctcaagaatt tctttctctg ctctttcgta tgtataacgt
     2341 tcatttacct tgattatact cttataaaac ttcgcagaat agattttgcc gctgtagtct
     2401 ccttccattt caactgtaaa agcaagtctg ttttgatttg caacgagact acaaagattt
     2461 tcggataact ctggagggag cataggaact actttgtttg caagataaac agaagtggat
     2521 cgactataag cttcttcatc taaactagaa cctggcttaa cataatagga aacatctgca
     2581 atatgaacat agaatcgaat tcttttttct tcttcaatga aactaatagc gtcatcaaaa
     2641 tctttagcag tttccccatc aattgtgatt gtgtataggt ctcttagatc tactcgcgag
     2701 ttccaatcta ttacagtctt ttcatttact tcttctggaa attcaagagg aattgaatct
     2761 ggatacattt gattgtaatt gtatttcatt aagattctat tgaaatcaat atcttcctta
     2821 tcacctgatt ctaacttaat aaaagttacc tcaagagtgt ttccatcttg aaaagcatgt
     2881 tctttaagtt ttactatcaa tatgtcgttt atttggatag attttgcgat atccgcaaga
     2941 agcgcttttt tggatagcaa accttcttta tcgtctccta ccatgtctaa aagttttcca
     3001 tgaatatatc caccttcagt ctcagtgaca cgcatacggt aaagagtgcg acctcttttt
     3061 agaattcgga ttacttcagc ctctagacgt tcctttttac caatgcctag tggagtaact
     3121 tccactgtat ctcctgacat actcgaatct aggaaattgg atggaataaa aagctcattt
     3181 ccggaaggta atttcaaaaa gccatctcct cgacggctaa tagatattat ccctgagaga
     3241 ctaaaaggct ttttaacttg aatcgttttt ttgtctaatt tcactaggtc ttctttttct
     3301 agaagccaaa ataattcttc gatctcagct tttttttcat cattgtattt tcgttttctt
     3361 tttccttcgt gtttcttgtg ttttttatct tctttagttt ctaggaattg tttatagaga
     3421 tctgattttg cgatctcttt tcctgctttt aattttaagt attctacgat actttttata
     3481 aatttatttg gtttcatttt taccttttta taatattata atttaattct tttttgagtt
     3541 taatttagtt tgatatagaa attatttcca aaatctctct attctgatct gagaaattct
     3601 cttagttgtt tcagtgagtt tttaaaataa tttgtatatg ttgaatgttc tacttttctt
     3661 ttgtcttaat aaaaatttat ttttagatct aattaacgcg atttttaatt cataattctt
     3721 ttagagattt caggattctc ttagaaatat gtatcgaaac gagagttgat ttcagttagt
     3781 gactttatgc aaaattagtc aagaagattc taattcgaaa aaaagactgt tcagattgca
     3841 atttatgtga aaatttgtgt aatagctgtg cttagagtca ataaaaccaa actaattaaa
     3901 ctccttattt tttgtatttt tgctattata actctttatt taacttggaa tcaattgaaa
     3961 acttcagaag tgagagggag agcacctctt gcggagaatg gttttctaaa cacagaacaa
     4021 tgggattttc aatctgaagg aactatcaaa ttagatggtc aatgggaatt ctattggctt
     4081 tcccttttaa ccccagatga ttttagaaat ttacaaacta agaatcaaaa caacctctat
     4141 caattagttc cttctacttg gaaccaatat aatttaaatc aagaaaagct ttccggaaat
     4201 ggatatgcca cctatcgttt aaaaataaaa atcaaagata ttcaaagaat ttattcaatt
     4261 aaaatcgtag atagtgcaac ttcctataag gtttggataa atggggactt gctagcaaca
     4321 gctggcgaga ctgcaaaaac aaaagaggaa atgatccccg cttataaatc aagagtagtt
     4381 gagtttaagc ctacttcaga tatcattgag attatttttc aggtatcaaa tttttatcat
     4441 tctaagggtg gtttatggaa tgcgattgaa tttggacctg ctcccttaat tgaaaaattt
     4501 cgagagtcac aagtgagttt tgaatttttt atgtttggaa gtctctccat aatggctttt
     4561 tatcattttg gtttatatgc tcttcgaagg aaagataaat caactttata ttttgggatt
     4621 atgtgtttta ttgttgcgat ccgcatctta ctgactggag aaagatattt ttttaaatat
     4681 gctgacaatg tagctcagtg gatacttctt gttaaactag aatttattac agtttattta
     4741 accactccag catttattta ttttttaagt tctatttatc ctgctgaagc aaagaagatt
     4801 atatcaagaa taacgttagg tgccggatta ttgttctctc tttttacaat aatgattgat
     4861 actacattag caacgcagat aataaaatac tttcaaaatt tactttttct tttaatagtt
     4921 tatacgggat atgttctgat taaaataaat cttaataaaa gagaaggtgc ggtttggata
     4981 acttctggac ttttagtttt tttatctgca ttaattaatg atctactcta cttaaataat
     5041 ttaatctata ctggtttctt attcccaatt ggattgtttg tatttgtttt ttctcagtct
     5101 tttattttgt ctatgcgatt ctcaaaagct tttacaaccg ttgagacaat gagcgataga
     5161 ctacttgcct tagataaaat gaaggatgat tttttagcga atacatctca tgaattaaga
     5221 acccctttga atggaatcat cggaattgct gaatcaattc tagaagatgg gagctataat
     5281 cttccttacg aaattcaaca caatctatac ttgattgtag caagcggcaa aaggcttaat
     5341 agtcttgtta atgatatttt agatttttca aagttaaaaa accgtgagat taaattacat
     5401 cttagaaaga tcgatctaaa gcagattgta gattttgtga ttcgactctc tgaacctttg
     5461 ttgttaaatc gagatttaaa attaatcaat gatatccctt ccgattttcc ggaaattgag
     5521 gcagatgaaa atagaatttt acaaattttc cataacctag taggcaatgc gattaaattt
     5581 acggaaaccg gtgaaatacg tatttatgct gaaattagaa atggtgtatc tacaatttat
     5641 atttctgata ctggtatagg cattccaaaa gacaaatttg aagtcatttt tcaatccttt
     5701 gaacaaattg atcactcaac gattcgagaa tatggtggaa caggtttagg acttggaatc
     5761 acaaagcatt tagtcgaact tcatggtgga aagatatggg tagattcaac tgttgggaaa
     5821 ggatctacct tctattttac tattcctagt tttacagaga atgacgttag acgggataaa
     5881 aatgttctct acacaaatgc agatttttat ataccaaatg taaatacgaa cctaaacatt
     5941 cctcttgatc gggtgattga cctccctgaa aaaaataagg caattataga aaaacaaaat
     6001 tatacaattc ttgcagtcga tgacgaacca attaatttac aggtaattgt aaatcatctc
     6061 aaagatctag gctatagaat cctaacagcg aataacggtc atatggcgct caaaatggta
     6121 gaagaagtgg aacctgatct tattttactg gatataatga tgccaaaact ttcaggctat
     6181 gatgtttgca aaatcctgag aactaaatat ccaattcatg cactgccaat tataatttta
     6241 tctgcaaaga atcaagtgaa tgatattagt tttgggctag aacaaggtgc aaatgattat
     6301 attcataaac catttcaaaa gaaggaacta atcagtcgta ttcacaacca actttctatc
     6361 aaacaggcga ttgaggataa taataaatta atttctattg aaaaagaatt acagatggca
     6421 aaaaaaatac aggcagatat acttcctgca aaagttccaa aattgcaagg actggatatt
     6481 catgctatct atttacctat gcagaccgtt ggaggtgacc tgtatgactt tcatgtagtc
     6541 aatccatttg aaatcggaat tttaatcgct gatgttgcag gtcacggtgt ttctgctgca
     6601 atcattatgg gtatggtgaa actcgcattc aaaatgcaca gagagtttgt aagtgatccc
     6661 gccgtgttat tacaaaaaat gaatgagacg ttatacggta ttattggtaa aggctatgtt
     6721 actgcaggat gctgctatat taatttaaaa gaaaaagaaa ttgtctatag tagtgcaggg
     6781 catcctgctc ttctattttt agatggtaaa acaaatctgc tgaaagaatt acaacccaag
     6841 ggcgcaattt taggagcttt cccaaagatt gtatgtagaa atttatctag tcctattttt
     6901 tccggtgata aaattcttct cgtcacggat ggaattcttg aagtgagacg agtcaatgag
     6961 gatggattat tttacgggga ggaaaaattc tataaatatt tagaacaaaa acgatcacta
     7021 agaggacaag aatttcttca tacaattatt gaagacgtta aatcctataa aggtatagaa
     7081 gatgaaactc ttggctttga tgatgatata tctattgtta caattgatat tctataatta
     7141 aacaattcta tcgttttgta aaagtattat taaaatgcaa agcttcgcct atctaatttt
     7201 aagtggttaa tctgtattag ctcctgattt agaaaaaatt gtttttttta gtggtaatgg
     7261 ctcttccgat attgaaaaag gattgtgtaa ctggatctat tctttgttaa gaagattttt
     7321 gtgctcaagt caaaattcgg ggtctcggga attccgacta cggctaagta gtacgaggta
     7381 gcttctgcat aatctacaga gtctgctatc actaagtaaa ctaaaaaggc tgagatagac
     7441 ataccaaaac taacacgata aagtttcagt tccgcccaat cttgtggatt cggtcgaaag
     7501 gaaatcttat gaagattctg acctttttct tggtatttgg tggtaagatt ggaatagcca
     7561 ggcacaagac catttgcaat gaagatttgg tatttagaga gtaggtagga gatatactgc
     7621 cgagttcccc catgagagga gcatttagat cgaaagagtt gatagtagcg atctggaatg
     7681 agtaaagtag agctaaattc agagtaaatg taatcttctc ttggaaaaga aattttacga
     7741 ttagaaatcg atacaccgtt tagatagggt gtgggttgag agttttgata taggtcatta
     7801 taaatatctt tccaatcata gtaagtattt tgttgattct catcgttcat atagacacct
     7861 ctaatgaata ggggaaaaaa attgagggaa tgtgcgggat attgtgaaat tttttaaagt
     7921 tgtttggaaa aatggaaaag acttagttag ggataggagg tttagaatga aaagaggttt
     7981 atttgtaatc tttttttata taagtttggc tctcgcgctt acagattgtt ttggaaatga
     8041 tttttctcag ataaaagagg gggatttaat ctttcaggaa tcaacatcag agcaagcgaa
     8101 agcaattcag ctagctacac attctagata cacgcatgca ggaatcattt taaaagtcaa
     8161 aggggattgg aaagttctag aggcggttca acctgtaaag tatactaaac tagaatcttt
     8221 tataaataga ggcgttaagc gacattatgt agttaagaga ctttctaatt atgatcaact
     8281 tgtaaatgaa tcgattttaa tgaaaataaa ggtagtagga aatcgatatt taggaaagaa
     8341 atatgatatc tattttgaat ggaatgatga tcgaatctat tgcacagagt tgatttggaa
     8401 aatttacaaa aaaactctga atattgaaat tggaaaatta gagaaactaa gtagctttga
     8461 tttgagtcat ccctatgtaa aaaacctaat gaagaagagg tatggggaga atatacctta
     8521 tgatgaatta gttatatccc ctgaatcaat gtttcaatct aatcagttgg tcacggtgat
     8581 tagtaactaa cattcgagaa aaaagttttc ttgctcaaaa ggacatctgg tcgagcagaa
     8641 ggatctttca gtatcaattt aaagattggt tatcttctcg gttttgattt tttagatttt
     8701 ttaattggtt ccacatctgt cttattagat tctaattcta gatcggcaag ttgtagactt
     8761 aaatcatcta gagccttctc tatagacaaa actgctttgt cttctacttc tcttgtgcga
     8821 tagactccat aaaacaataa attataatcc tctgttttat caacgcttag atgaattggg
     8881 tctttcttcc ctagtttgat tttacagtct atatttgttc tgattgttcc tgactttgca
     8941 agtaatggaa agtaaccgct tgctggccaa aatcccggaa taaaagtagg ccaaaatatt
     9001 ttttcgtttt ttttctcatt ttcactgtag gggatgtaaa aaaagaaaaa attctggtta
     9061 tagggataac tgccaactct cgctaattcg cttacactaa ataaaatttc tattagataa
     9121 atatcttctg gattttccaa cattgaatct ttccacttta cttccgaaaa acgacctgag
     9181 tttagaatgc ttacacgaat tgcttctttt aaaattagtt tatacttagg ttgtatctct
     9241 ggggagtctt ctattgtaac cataggagag tagatggtcg gctcatctaa atagacaatt
     9301 ggttttcctt ctcgattcat cggaaactct ttatagccta tcgttctatt taataagtgt
     9361 gccctgcatt gaaataaaaa tagaactaga aacagtattt tcattagaag ctcactccta
     9421 aaataattcc atagtttaat tttactttct ctgaaggctg aatcgcattt tttaattgct
     9481 cttgtaaaaa taaaaaatcg gaaagactca aagaattttt gttcagaaga gatacttgta
     9541 tattataatt atattgaatc tggtaaagat ttaccatccc gaactctact cctgcaatta
     9601 caccgctggg taaaatatac tttaaaccaa gaccaattcc gtagtataga gctggtttag
     9661 cgtcaaccga catatgtagg ataggcaatg gataactagg cttatccatt tgataggctg
     9721 ggttgaaaat accattttca gaaccattta ttaaagtaga gccctgcgat ctccctagat
     9781 agactgggat ataaatagga agatctaatt tgataagtgg atagtaatgt aaaaaaatat
     9841 tcgttcccat tttgggaatc aattgagtgt taccataatc ttgagaggga acagatcctg
     9901 cttctgtgta gactcctttg ctcaaggttt tagttccatc ataatagcta taatcttcac
     9961 tggtttgtct attttggttt tgggaaattc caacagaaaa gtagccattt atgttataac
    10021 ctaaactgaa taaagcaaat ttagaaagac cgcttggtag accagtcaca atgttatatt
    10081 tatatttgag tctttgtttt tcttgtccat aaggtattag atcctcttca ggtagttttt
    10141 ggtttccaat tatttctttg ggtagaggct gattcgtgtt atccgcgtag ctcaaaatgg
    10201 gtgttaagag tagaaggaaa agaaaatact ttatcataaa tcgaattcct ttttgaatga
    10261 aaaagaaaat ccactatagt ttttttcata cgggtgtttc gaagtagatg gagaaatctg
    10321 aaactggaat ctagtatccg aggagatata gaaagcatct acaatattaa aaacataaat
    10381 cgatgccgct ataaaatacc aagaatgaga gctatatcca tattctcgac tatcgttttc
    10441 taaggcgcta cccttataga ttccaagaag gaatacagga agaggtagat ttaaataatt
    10501 tgcgatagcc gctccagcat agaaatctct actttgttga cttatttgat gcgagtaaat
    10561 attttgatct cgaccaatcc aaagagtaaa tagaaaactt accgcaaata aaaaagcctt
    10621 tgtgtctttt ttaaatgccg cctgtccaag gcctggaaaa tagaaagatc tttttgcctt
    10681 gtctaagttt ctttcttttc tttctgcatc agataaattg tcttgaatca atggaatacc
    10741 tgtttccttt gcggaaatag aatttgattc caaaaatatt tttacttttt cttgaaacgc
    10801 tttcatctct tctagatcac aagaaaggtc tagacattcc aaagcgattt cattgactgc
    10861 tttgcttgta tttgcatcca caatataaag aatacgatag gagccatctc ctattgcttt
    10921 tgcacttccc cagataaaat aatctccccc tagacttttt gtttctgatg agagtttttt
    10981 aaaagaagga attgtgtcag gatatttttt atcaggttgc tccgaaatta tttgaagtcc
    11041 tagtccttgc aaggctctta gaattttatc tgattccttt gttgctagtt cctttgaaat
    11101 tccttgtccc gctaacaaag gatatacttt tatttttttc tctccatctg catttagatt
    11161 ccaactgaaa caaaatagag ataatataaa tagatatatt aaaaaatttt tcattgaaaa
    11221 atcaatctta cttcctgtaa tttaattttt caatcttttc cttgatcttg aggatgcttt
    11281 ttaagcctac tttagggagt atgaaaaaca ctaaccctaa agcggcaaat gggaattata
    11341 ctctagagag tcttgcttct agaagttgaa ttgcttcatt gatgttcgca tttccaatca
    11401 ctacattgta agcacttgta agaattggat acttttcaat attgagttca actagatttg
    11461 gaattgcttt taaaccataa taaccattgg acattttact tggtttatta cctgttacta
    11521 gttcatagcc ataacgtcta tccctagaat ccgatccaaa gcaagcgagc atgaagtcag
    11581 taagaccaga tagaccattg aatgtctgtt cttgcccacc aagctttata ccaatggaaa
    11641 ccatttcttt aaagaagcgg tttgataaat ggaataatgt attgtccaca tttccgccta
    11701 gctcttcttt gaagtaacct tctacgagac ccatacaaag agcataaata ttcttcaatg
    11761 ctcctcctaa ttgaacacct gcaacgtcag ttgaaatgag cgctggtcta gagaaaatat
    11821 atccggttgt aaatagtttt actagttttg gaatgatctc aggatccctt gctgcgattt
    11881 ctaacccaga aatttttctt tcaatgatct gttctggata acttgcccct gctgctactg
    11941 caagtctaaa tctttcgatt ccatatactt cttctaggtc tcttaggatg agacctcttg
    12001 gagaatttgt aagacctttt atcacattga cgataggcga tttatttttc tttaatttat
    12061 ctaaaatatc aggataaact ttgtcgaagt cccaaggatt tgttccttga atgaatagag
    12121 ttgcatcttt tattacacta tcatctgcgg aaaattcaat gttaggtgga agcttataga
    12181 gtggataatg ttctacgtct cttctttctt gatttgaatt tttacaatat tctttgtctg
    12241 ggtgataaat agttacattt acatttttat tggaaagtat agcagcaata gctactgacg
    12301 cattgctaga gccaatgact actatgtttt cttccggtgc tttcggagtt tgaattaata
    12361 tatttttatg tttcgcaatt ccatctccat tataaaggtt tcgataggtt ccatgcttat
    12421 gacggctcaa gttagaaccg accataagcg ctaagttgtc aatcaggaat tgtttcttat
    12481 ctcctacttt tggaaatttg atttcatcca attcaacggg taatttgtgt ttgccgtgaa
    12541 gatctcctac aataaccggt ttacctatca caagctttcc attggcggga ttaaataaaa
    12601 atccagaagt tggtaaaatc ttatctgttc cttctagaga gattggtaat actactttat
    12661 tcgccacata gtgatagact gtatcgacaa atggcataag tcttccatct ctagatctgg
    12721 ttccttccgg aaaaatacaa ataatcttac catctccttg cagcttttgc gactgtcgga
    12781 aggcacgcat gttgattttt gtcatgaggt cggaaagact tggattatct cccatgtctt
    12841 ttttagagca aacaagtaac gtgccaaaca tataaaggcc gagtcttgta aagtctggct
    12901 caaaggctag acgtcctgcg ataaacacca tctgctctgc gatctgtctt ccaagtggac
    12961 cggaagaata aaataactga aaaatgcctg gagcatctag gtgacttaaa tgatttgaga
    13021 tcagggtaat tggaaatttt cctattaatt tttctactat ctcaagattt tcctgacctt
    13081 ccactttgaa gtttttcata ataggggaga aaaactgcat cataaaattg cgagcttttt
    13141 cttctccctg ggtataaact ccaacttttt ccagttcatc tggattttta aagatattca
    13201 tcaccggtgg catcggtgta gtgctagaga gataaagaaa cttttctaag agttgtcttg
    13261 cttcttcttc tgttaaacca gattttacaa aattgtggat attctcaaaa aattccttat
    13321 gccatctacc aacgcttgct tctttttcag tcataaatgc ctctacctgt tcaaatcttt
    13381 ccaaattaga tttgggattt gcaatccagt caactagaaa aaaatagaaa tgaatttttg
    13441 aaggaatctg aatcgattca tccatcaaat cctagttgta atatattgtg ttcttaattt
    13501 attggaagag ggattagaag acagttgaga aacgaccgca atacaagctc agaattaaaa
    13561 tggataccgg acgggtttca ttttttagat catgaagata gcctcagacg aaaaaaaatc
    13621 ttagaccaaa tctatgaatt ttttacttcg agaggttatt tagaagtcac tccacctagt
    13681 ttcgatttta caaattcctt tttaagccat gtagcaaatt cagaagaagc tcgaattcta
    13741 aaatctcgcg atctctctgg tagagagatt tcacctagca tcgatttgac gctacaagta
    13801 gtgaaaggtc tagcgggatt tggagttaaa aaaggaaatc aaaaagtttt ctatactgga
    13861 cgtatcgtta aagacaattt caaaaagaat gcggatagaa gagaaattct gcaagcagga
    13921 gcagagatca ttggacattc caatgccaat acatttaaaa tgttactcac tcatatcagt
    13981 gaactagttc ctgtattagg tctcaaagaa aaaatcactc tcgtattggg aaatactgcc
    14041 atctattcaa atattgttga gtatctagaa ttttcagaag cagataaaaa tactctctcg
    14101 aacttactct atcataaaga agaaaaaagc attcgagatt ttctagatct aagagtaaag
    14161 aaaaaagaaa ttcgagatct aatcttaaaa ctaattctaa gctttgagct aaaagaaata
    14221 aaatcagatt taatttcaat ctcaaaaaaa tataaaatca aattagaacc gttaattgat
    14281 gaaacagaag aaatctttaa atatgcaaat cattttaaaa atttagatct atgtatggat
    14341 tattctctta tacgagattt agattattat actgggttcg tatttcatgg atactatgat
    14401 cgaatttcct atcctgtcgt aacaggtgga gcctatgatc atttatttga aaaattttca
    14461 ggcgaagaaa aaagagcctg cggctttgcc gtaaacattg atatcattga agaaataaat
    14521 agaagataag aggataatag taatatgcca gcagatttag tgattggtgc acaatggggt
    14581 gacgagggaa aagcaaaagt aatagattat ttaagtaaag agattgatat catagctcgc
    14641 taccaaggtg gtgcgaatgc tgggcataca gtagtcgtgc atggaaagaa atatgtgttt
    14701 catttagttc cctctggtgt gatttatcca aatacaatat gtgttatagg aaatggagtt
    14761 gttttagata cagattatct gatgaaagaa tgcgatgagc tttgcaaaga agacttcgat
    14821 gtgtataata aactctatat tagtgatgct tgtcacgtaa tccttccact tcatggaaaa
    14881 attgattctc atagagaaag aaattctact cctggaacga aaatcggaac taccggaaag
    14941 ggaataggta tttgttatgc ggataaaatg atgagaattg gaattcgcac gggagatgtt
    15001 ctagatgaag aaactttaaa agataaacta aaacattttc tagcaaagaa aaatgaagaa
    15061 ctagtaaaac tctatggaga agagccagtt caatttgagc caatctttga tagcttgaag
    15121 aaatttgcag ataaattcaa aggcaaaatc ataaacacag cctacttttt aaatgaagaa
    15181 cttcgtcgcg gcaaacgcgt gttactcgaa ggggcgcagg gcgttggtct tgacattgat
    15241 ttcggaacct atccatttgt aactagctct aacccaacga caggcggcgc actcactgga
    15301 tcgggaattg gatttcaaca tctaggaaaa atttacggaa tcagcaaagc atacgcaact
    15361 agagtaggag aaggaccttt ccctactgaa ctttttggag aagacgcaga gaagatgcgc
    15421 acgataggtc atgagttcgg atctacaact ggtagaccaa gaagatgtgg ctggtttgat
    15481 ttagaaatga ttaaacactc aattcgtatt tgtggaatca atcaacttgt aattacaaag
    15541 attgatgttc tctctaccta ttctaaaatt ccagtgggtg ttggttatga actaaacggt
    15601 aaaaaattag actacatgcc atcctgtgga atggagaaag ttaaagtaat ttatgaagaa
    15661 cttcctggtt ggaatacaga cataacaggt attaaccgct ttgaggacct tcctaaaaat
    15721 tgccagtctt atctcaaata tctaaatgat aaaattggag ttcctatttc tatcatctct
    15781 accggtcctg atagaaaaga tacgatagtt ctctaatatt taaaagcttt ccaccgatga
    15841 gcacagataa acaccgatgt ggtttttatc tgtgtttaat gtttatttta tttggataat
    15901 ttttaagact acgaagatcg cgggaggatc gagaagataa tgagattcga aaatgattcg
    15961 ctctaggtgg tattctagtt tttgattcaa tccaagatga ttacgttgag ttttgcaaat
    16021 tatctaatat ggattgagga aaagaaaaca atgttgctta tcctcctaca aggaacgaga
    16081 agaattcatt caaaccgact ccacaaaatc agccttagga atttaaactc gatgcgttag
    16141 atgaaaagtt aacaaaggat tggagaaagg aaagtgagtg tatctttgtt aacgaaagtt
    16201 ttgtgaaata tcgccataga cgtgcattcg gggactcgcc ccttcagaga aatcgtattc
    16261 aataaaaaat cccaactgga tttttcgagt taccaaccaa tttcaaaatt ctcattccta
    16321 actaactagc tacttggtat agtggttcgt atagtgttgg tatagtccta cctatactga
    16381 tagggatcta ttgctatagc aattcacatt gtaagtgtga gacattcgtg tggcaataca
    16441 aacgcaaggc ttttctcttt tacgacataa tattttaaat ttgttttggt ttatgttaca
    16501 taatcaatag ataattatgt aacattaata agatgattta tgtcgcacat atttctacta
    16561 tcgattcttt gaggattttg aagcggctaa tcattctctg acgctataaa attaaaaaaa
    16621 atattaccta tagcgacata atggattcat atcggcaaaa aaaataaaaa attaacgaga
    16681 aattaaattt tttgctttaa attcttcatg gttcaattta tacatcaagt cgataataaa
    16741 attgtctacc tttcgctggt agctttatta atagcttata taaaattaga ggtttttttc
    16801 gatgaatgta gcaattaagg cagtaaataa caaaaaggaa atcaatccta atctaattct
    16861 agaggcggtt aaaaataaca agccgatcac gatcaaaaca tactatctta cagagtccac
    16921 tgaagaatat atcagaagta ttattcagct cgtaatgggg cgtgtgggta aaacagaaaa
    16981 tattgaaact tgctacaatt cagttcgcgg tcttgttgta aatgcgacac gcgccaacat
    17041 taaacgtatt ttatttaaag agatgggact cgatatcaca aaccctaccg agtatctgat
    17101 aggtatggaa aaaattgaac ctaagttaac tgaaattcat tttccaaaat acaaagcaga
    17161 actcacaaga cagaagctta ttgttgaaac gacctttaat tttaatccag atatgttctc
    17221 cgtaaaagtg aagaataatt ttattctact tccacatgaa tatcaagttt atggaaagtc
    17281 ctcatctaac gacaaatcct ctattaacgg ttctgttgtt cgagtaagag aagttatttc
    17341 caatggtcag gttgctcaaa ctccatctaa agttgtatca ctcaatcaat ataaaatcga
    17401 ccaacaagtt ctaagtcatg taatgaatga agatacaaaa gaaactatct tcaagttaga
    17461 tatcgtatta aaagataaaa acacttttaa cactgaaaac tttggacaag aactcaatgc
    17521 attatttaca agctacaaag agaaattcga taaagtattt aaagaaaatg tagaaggtct
    17581 tgatactaga gactatttaa ctgtttcaaa ggatctagca aagagtctaa ctgggcttcg
    17641 aggcagaggt gactttttaa atttatatca acgtattcaa aatttagatt cccagttgta
    17701 taaagaacaa caacaactac tcgcaaacta cggcgaacat gtatttaccg caattcaaga
    17761 taaactctcc aaagcaaagg ctgaatccaa tgaatacatt tcacaaatcc aacgcattgg
    17821 aaaagaagtc tttgaaatca ataagaaaca aattgatgaa tttgctcagc aaaaagattt
    17881 cgaaatcgaa aaacaaatca aagatagcat tgatcgacat aaaaagaaac tagaagagtt
    17941 taacgactca ttagataagc gtattgaaaa tcagtctatg attatcaaaa gccaattgtc
    18001 gcaacaaatg gaagatatga atcgttctag tgcagatctt ctcaaatcag caaagaatga
    18061 aattgccaaa gttaaagaag aactaaaagc agatttagaa agtgaaattc acattgctga
    18121 actcatgaag agtgatttgt tccaagatat caaagcagac aaagaagttt tagaaagaac
    18181 tctcgcaact gtttctgaag agattcaaaa gattgagaaa tttcatacaa ataaagacgt
    18241 tatggacaat ttgattgctc aatcggaaga agctttctgg aaaatgtcct ctaatatcga
    18301 agtcatcaaa gctaaagaag aaaatatcaa tgaatacatg aacaatatta atcttcttga
    18361 aactgctatt cgcaatactg aaagagaaat tcgagaactc aatgatgaaa aaacagaata
    18421 cctaaatgaa aaaggtaaaa tggttcaaac cattgacact cgtatgaaac agatggaaaa
    18481 atttggagaa gagctcaaac agaaattagt agagatcaat agctttgagc gcaaaattaa
    18541 tgttatctcc ggtgctcttt ctgaacaagt aaagaaaaca aaaaatgtgg atgagacctt
    18601 agcaaagttc tccaaagaag ttgcagcatt tgaaactaag aaaaatgaac taggtagatt
    18661 tatcactgaa gtagatcaaa aggttgctct catcaatagc aaaacaacag atattaaact
    18721 tttagaagct aagttcaatc atatagaaag actcatgatt gacttatctg cacgtcataa
    18781 gcaaatcgct actatggaag ctcgcttaga tgatgtgaag ggcaatatgg aaaaactcct
    18841 ttcacaagct gaaatcaaga tgaatcaaat gtcctccgtt atcaataatg cggaacaaaa
    18901 atctagttct aagccaagag caaagaagca ggcaagtcca aagttttcat ctgtattgaa
    18961 ggatttaaag gaaagcattc taaatttaaa gaaaaagaaa ttaagcccac aagaaattgc
    19021 ttctgcactt gatattgatg aagaaatggt ttctatgatt cttgcaatgc aataggcatt
    19081 gttgaattat tcggatatag aaaaaagatc tgcagagaaa tataatttct ctgctgtatt
    19141 actattaaat ttatttcact cagcaaattc tatttacaac attaatagca cctagcacaa
    19201 cagctgggat tccctgaccc ggaaagactg tatctcctac catatataaa ttttggaaag
    19261 gtgtgacgtt aggtgtcatg aatattaaat tattctgtat agagtgtggt attcctccca
    19321 caaaaccctg atgtcttcct gtgtagtcag caaaggtttt aggcgatcca cctaagggga
    19381 aaattttttg tttcatttta ggaaaatgaa aatcaaattc gccaagaatt tctttcatta
    19441 caaactcttt tttctcttta tataaatctt ttgaaagatc tgaccattct actggatttg
    19501 tatgggttga aattgtaaca gttctatagc cgataggcgc tttttctaaa tctccttctt
    19561 ttgaaaaaga aacaaagaaa gcaccagcct caataaacgg aatctttttt ctaaaatgaa
    19621 tttgatagta aagagaatcc aatatttcat tgttttcaac tgcaaagtaa atggtaaatg
    19681 caccagatgc attttttaac tttgaagact ctttcttaaa gtaggactgg atcttgccct
    19741 cagtgacttt cgccatatct gccgttgtaa ggttagaaat aattcccctc gctttgtaaa
    19801 tatttccttt ttttgttcta actaaataac aatccttgtt gaaactaatc tgaattactt
    19861 cttcttttaa aaaaatttca cctttagatt ctttgaaatg atttagtaaa agttgtgccg
    19921 gtttatacat accgcctatc ggataataag tctctttggg ataagaaagc cccatagcag
    19981 aagttagcat tggagataga tccactctat tctgggttgt gatgagtagt tgttcgttta
    20041 aaaattgaat aaaatcatct ctttgtaatt gaagagattt taattcatcc aatacagatc
    20101 gaaatagttc cttcagcaga actattttat tgaaattttt aaaacgaatt agggaaatta
    20161 ggtcagatac agttctaggt ggaagatttg aattttgatc gataaactcc caagctaatt
    20221 tttctaatga attaatttta ttccaaaatt gattcatctt taagtctgga aaatgcttgt
    20281 taacctcatt cacccatttg tctagatcgg aaaatcttgt aattttctga tcattcattt
    20341 ttattatcat tcccggatct accttgtata gatctggagt aatttctaac tcatgaaata
    20401 atttacctaa aggctgattc ggaagtatgg cgctaaatgt agtagctcca acatcaaata
    20461 aaaaatcctt tcgtttaaaa taagaagcac atccacctat aagggaatgt gcttctagaa
    20521 tacatgtagc gaatcctctt ttctgtaaca gagatgcagc ggctacacct ccataacccg
    20581 caccaattac aatccaatca aacatataca attagacaga aaaaaacttg caatataatg
    20641 atctatttat attcttctga caaccttgga gatactttta gactatgcct gacataaaat
    20701 atatttgtct ttcagatatg cactttggtg cacagaatag cttactcaca aaattgacac
    20761 cggatggatt aaaaccaaat tttttagagc ctagtcccgt tttaacttct ttaatagatc
    20821 aggttaaact cttgttaaat gagaatacca ataaaaaaga gaaacctgta cttattttat
    20881 taggtgatat actagaactt gcactttgtg aaacccaaga agctggtatg gtatttgatc
    20941 gttttattga tcttatactt ccgaaagaca aagagctatt tagtgctatt tattatatac
    21001 cgggaaatca tgatcaccac ctatgggaaa tagctcgtga atcacaatat attagatata
    21061 tccaaaggaa agcggataat agtattttgc ctaccatgtg gcatacaacg aatatatata
    21121 tggaaaacca ttcctatcct gtaacctgtg attttatgaa tgctattatt cagaatcata
    21181 agcatctaca aaaatttgaa gtgcaggttg cctacccaaa ttttggaatc ttagataagt
    21241 ccggtcagaa atctgtatta tttcatcacg gacatttctt agaggatatt tataccctta
    21301 tgagttctat caaaacttat atttttccag accaaaaaat tcctgacgat atttgggata
    21361 ttgaaaaaga aaactttgca tggatagatt ttttttggtc tacaatggga agatcgggac
    21421 aagttggaaa aggcgttgag ctcgtttatg ataaaatgac agacgaaaat aaattttcag
    21481 agttacttca taatatttct gtaaaaatta cacaaaatat aaaaaagagt gaaagtgcag
    21541 aggcaactac cttttttaga agaatgtcta agttcttttt aagatttata ccaggtagga
    21601 aaatagagaa aataatttat gcatttttaa agataacact tggaacgatt gcaaaacatg
    21661 aaagaaccga ttctggagga agtctttcgg atgagatgcg ggataagtta gttttctatc
    21721 ttaaccaaac attaaaacgt caattaaata gagagaaaaa agagaatcct cctaaggaaa
    21781 tacagtttgt atttggtcat actcacaaac cattttcgga agttatggct aatctagatt
    21841 atccgcagaa agtccaagtt gtcaatacag gcggatgggt catcgatacg gtcgatagaa
    21901 aatcaaccta tgggggagct gttatcttaa ttgatgaaaa cttaaacatt gctcccctcc
    21961 aaatgtatag agagagttat gagagtggaa aacctaaagt tgctatacta gaaaatacaa
    22021 tcaataacga atttactaaa aaaattaatg agattattga acgtgagaag aattcttttg
    22081 attctttttc taaaattgtt gataaggaaa tagatctacg aattacgaat ttaaaagcta
    22141 aaatcaatcg ctaaaaatag gagaaatagg atgtatgaag cagttgtaat tgggagtggt
    22201 tttggtggcg ctatcaatgc ttgccgttta agcaaaaagt ggccgaatgg gaaagtattg
    22261 gttttagaaa gaggaaaacg ttatccgatg tattccttcg caagaactcc ccatgaaatg
    22321 tcaaagaact tctggaatat tccatcagaa acaaaaggtg gcaatccaga ggatgaggaa
    22381 gtgcatggct tatttgatat tcgaaacttc cataagatgg atgcagtgat atcagcagga
    22441 ctcggtggag gatctttaat ttatgcaaat gtttttctag aaccaccgga ttgggtcttt
    22501 aatgagagat ggcctgaaac gtgtaaaaaa gagaagttac ttccttatta taaagtttca
    22561 aaagaagttt tgggcgcgag accaattccg actaacgatg acccaagaag aaaaattaga
    22621 cgaacccaga gatttcaaga atttgcaaaa gaaatgggtc ataattctca attattagat
    22681 ctaaacgttt tcttcgggaa tgattttaaa aatccaactc ctattggaat tcaagagaaa
    22741 aataaatacg gagcgattca gacatcctgc acatattgtg gggaatgcga cgttgggtgc
    22801 aatactcatt ccaaaaacac attggatcta aactatcttt ttgtggcaga gaatgttcat
    22861 aaagccgaaa tacgcacaga acaattagta aaaaaaattg tcccacttga cgcaagtggt
    22921 aaagaagatc ctaacgcaga tggaaaaaat ggatatcgaa tttattttgt aaatcttaca
    22981 aagtcacgtg gaaccgatag attagaatcc gtagacagta aaagaattat tatatctgct
    23041 ggatctcttg gatctacaga attgctttta aagtgcaggg atgagtataa aactttacca
    23101 aatctttctt ctaagttagg agaatatttt tctggaaacg gtgacttttt atcctttgta
    23161 attcgaggaa aagaagaatc caacccaaat tatggaccag ttattacaca agcgattgat
    23221 tttaatttat acaaagaacc agataaaaag agagcatttg tcttggaaga tgcgagttat
    23281 cctgcttttg ctgcttgggc agccaatacc ttaccaagca cctcaatttg gaaaaatcta
    23341 gtagctgcaa taaaagatat ttggtttaag attacacgag agggaagtgc ttacggaaga
    23401 tcgggttata tctttaatga gatgcttgcg aatgacgttt ctcaaaattc gagcgtgctt
    23461 ctttttatgg gtgtagataa ttccaatgga aaaatgtttt taaaagatgg tgaattggaa
    23521 atcaattgga ctcaagaaga aagcatgcct ctctatgata aaattttaga tatatctaag
    23581 aaattttatg aatgggcaaa agcaaaattc tggtttcctc tttttaattg gaacgcaatc
    23641 tcaaaggata atgtaaccgt gcatgctcta ggtggttgta ttcttgcaga ttcaccagac
    23701 aagggtgtta caagtgctga tcgaaaaaca tttggacaag tatttggtta tacaaatctt
    23761 tatgttgccg atggagctat tgttccaacc gctgtaggag caaatccaat tgccacaatc
    23821 agcgctcttt ctgagatggt tgctgaaggg ataacgggca ttaaaccaga ctcgtctcta
    23881 aaataaaatt caataaaaat aacttttctt cttactaggg taaattaaag tgaattatca
    23941 tgagtagaag aaaagttccc ggtccaccca aaagccattt tatatttggc aatctaaatg
    24001 agtttcaaaa gaacccacct gaatacattc gtaaggttac aaaggaatat ggaaatattt
    24061 ccgccatcga tttagctgga ttcttatgta taattgtaaa tgaaccagaa gtcatcaaag
    24121 atataatgat gacgaatcaa aaacaatttg taaaagcaca tgcttttcgt atttttaaaa
    24181 aacttcttgg tgaagggcta ttaacaagtg aaggagaatt tcataagaga caaaaacgtt
    24241 tagcacaacc tgcttttttt aaacaacgaa tacactccta tggtccggtt atggtggatt
    24301 atatcaatag aatacaatct acatggaagg atggtgagac gattgatcta catacacaaa
    24361 tgactgatct aacgttaggc gttgtgacca aaactctttt taatacagag gtcggtacag
    24421 ctgaatcacg tgcaatagcc caatctataa ccgaagcatt agaaattttt gatgaagttc
    24481 ttgcaagtcc atttgccgac atcattctaa agatcccatt tcttcccatc accatgcgat
    24541 tgaaaaaagc aatcaagaaa ttggataaga ttttatatca gatcattgat gagcatcgaa
    24601 agcatggagg ggatcaaggc gattttattt caatgatgat gttagcaaaa gacgaagagg
    24661 gaacaggtag catgtcaaac aaacaaatcc gagatgaggc tatcacgatt ttcctcgcag
    24721 gacacgaaac gacagctaac gctctttgct ggacatttta cttactcgca aaaaatcctg
    24781 agaagagaaa aaaatttcta gccgaaattg attccgtttt gggaggaaga aagccaacag
    24841 tcgaagatgt gccaaaactt acctatactc ttatggtttt ttctgaagcg cttcgtatgt
    24901 atccgcctgt ttggtggacc ggtcgggaaa atttagaaga atattcaatt ggggattata
    24961 aatttaagaa aggaactatc ttcgtcatta gtcaatatgc gatgcaccat gatgaaaggt
    25021 attattctga tccttatact ttcagacccg agagatggtt gcctgaagag gcagatacga
    25081 gaccaaagtt tgcattcttt ccgtttggtg ctggaattcg tatgtgcatc ggaaatcaat
    25141 ttgccgagca ggaggctata cttgcattag catctctttc acaggattgg ttactgcaca
    25201 tcgaggaaga aaaacaagta ttaagcacaa tgctaacact aagaccaaaa gatggaatga
    25261 agatcaaact agaaaaaaga aagcgttagt tctcagctct cttttttaaa gcgagattca
    25321 tttcttcatg aactcgcttc acatctgttt caatatgttt ttttgagcta attgcgaaaa
    25381 agccactgaa tgtagcagca tgggaaaata cagttttact gttatttatt tcttgaaatt
    25441 catacacatg atggtcagaa aagcctggga aatttccaac ccaagtgatc ttttttttat
    25501 tctcaaattc tattattttc ataagaattg gtaaatataa atgattataa aaataagcaa
    25561 attcaagaat tgttttctcg ggtgcaattt caccataaac tcttttaata aatggattcc
    25621 attctgaata tttcgaggta tccataaaca aattccaaat cttttcaaca tttgcattga
    25681 tttcaactga cgttgtgaat tgtatacttt tgctcatttt atatatccta tttttctgtc
    25741 agtttagaat aacctttcca ttgatctaag tttttcatta gcccatctac tctttctaaa
    25801 aagacttgtg ttgccttatc ctctttatta agcaatagaa cttgatcgaa gagttgcttt
    25861 gcttcaacga aagatccagc atagaaaagt tggacagctt tttcatacat ttctttagat
    25921 gcaagcttag cttttaatat atcctcagga tagtagtcta acacttcgta aactacaact
    25981 ggtagagtct taccctttac aactaccgta tctaaaagcc taaatttata cttttctttt
    26041 gcttgcatct tatttaatgt atcctcgcta atgataatac ctaccccata ctgtttcgtc
    26101 atactttcta agcgagaaga aagatttact gcgtctgata tcacggtcgt atccattctt
    26161 ttatcttctc caacggttcc gagcatcaat tcaccggtat gaattccaat tccaatagaa
    26221 attggtccat atccttgatt tttacgatgt tggttgtata aagtgattcc cttcatcata
    26281 tcgatagatg ctttgattgc gtcatctgca tttcttggga ataaagccat gattgcatcg
    26341 ccaatgtatt tatctatgaa accgttattt tcccgaatag atggtcccat tctttttaaa
    26401 taagagttta taaaattaaa attctcttct ggagtcattt tttctgaaag ttcggtgaaa
    26461 gagcgtatat cgctaaacaa tacagacatt tcttttcgaa tttgatcccc gagttttaca
    26521 tctacaatac tttctcggtt taagaatttt aagaattctt caggcacaaa tctttggtat
    26581 gccgaattga gttctgacat attcgcataa agtcttgcat tttcaataga aattgctgct
    26641 tgggaagaaa gtagatttaa gattctaagt ctttcttttg taaatgcgcc agttgaaact
    26701 ttgttttcta aatacaatac acctacaacc tttccttgtg tcatgagagg agtgcacata
    26761 acagatctag gcttcgcttc ttgaatgtat gcgtcagttc catactttgt atcaacaata
    26821 gcatcatcta ttacaacatt ctcttttgag cgaactacaa agtaaatcat acttgtagga
    26881 catatgagag ttgaaccatc gatcgcaatc gattctaaaa tttgaggaga tttacctgtt
    26941 tcataaattg cttctgcaaa gaattttttc ttagattcta gaattaaaat tcctctgtcg
    27001 gctcctgcat tttgcaaaag tgtgctcatt aagtttctta gaagtttatc tagaacgatt
    27061 tcaccagaca tagactgagc tgctttcatc acggaagcca aatcaaatgc ctcgttcgaa
    27121 ttttttgtta ccgaagtaga tgctaccgtt gttgaagaat tgctgattgt tgtatgtgca
    27181 aatgttgttg ctccttttgc ctccactcgt ttgagttgga ttccttggta aaccgtttcc
    27241 atctgtctta gttttgcagt agctccccaa atattatatg cgttatacgc attcattaag
    27301 taatgagttg ctattactct tctaccacgt gatagataat attttcctgc aagctctgca
    27361 cagattgctt catcattcgg aaaacctttt gcctttgcga gctctagagc tttgtcataa
    27421 tttaattctg ctttttctga attgttttct aatcttcctt gttctgctag taataaatag
    27481 tatctgtgct catggttaag gggagcttta tcatgataga atttcatttt attaagattt
    27541 gatttgaatt tccagtatga taagccggag ggctttttta ttaaatttga aagatttaat
    27601 ccttcataaa aatgaaaaat aggaacgtta attgtggaga tatcgctatc tataaatttt
    27661 ctatactcct ttgctgattt tatccccact tcatattttc caaataaata agaaagaatt
    27721 aattcttgta gatagaaatt gattatagag ctgttatcct tttgctcttt taagcgtttt
    27781 aaatcttctt ctctatcaaa gaattctcca cttaatatgg atgggtcttc tactttacca
    27841 agcaggttgt ccgttatttg acaaaaagaa gcacttaacc atagagcaga ttcctgacgc
    27901 atacttttga ttttctttga atagactgca gcttctttat ttagttgaga caattctttt
    27961 ccactataaa aggaatttct aatataatta aaaattgcat atccagcata cgttaagtcc
    28021 cctgtctgaa gagcaatttt gtatgcttcc ataaaattct taatggtctg tcccatactt
    28081 tctttccagt gaactccgaa aagattcatc attactagaa tggatgcgta gaattttgga
    28141 ttcgggtttt tctcgtaaac tattaaaacg gcctgacaga gtttgtatcc aacatctaac
    28201 tttccaagga ttgcacataa aataataata tatccggcaa gcagaatagg ggaaatgtcc
    28261 gatacacctt tcttaaatgt tttatctaat gcctgaaata gaatcagcgg gtttagatct
    28321 ggcattgcaa ggtaagccga ggctgaaatt gttgctagaa gattgagtcc tgcataaagt
    28381 tttggatcat ctaattttgg aagtttggct aactctaaaa gttttttatc tgagaattta
    28441 acaatcgctc caaggagtcc cttggcaaca tgtagtttat ttggtcttcg tggaaatttg
    28501 aatccaagtt gatctaatat tggaaatgac gtatcaagag cttctattaa cttgttttga
    28561 gcaatcagag atcgaattct aatttcagat attttagttg tagcaagaat atctttgcag
    28621 tgtggtaaaa taatttctac taactcttca cattcttcat aatttgtatt tatataggat
    28681 gcctctgcta tttcaaaata gagagaaaat actaaatcat aattctttgt ccaaaaagat
    28741 ttatcgctgt tagccgcagc ttcttttgat gtaggcagac ctagtaaacc taaacctgtt
    28801 ttcccataag atattgctgt ttcataggca gcagagagtt ttgctttaga ggtagctttt
    28861 agattgaggt tggcaatttt gattttatct tcttctttga tatgaacaag accttgattg
    28921 atatgattga ctatatcaaa tagatgatcg tccaattcct cttcgcttgc tttggataat
    28981 aaaaagtttc caatttccca atggactttg gatttttctt ctacagggat taacgtgtag
    29041 gcagcttgct gaattctatc atgcaagaat ttaaattctg catttccatc atatttgatt
    29101 tctaaatcaa taaacttata attttcattg attggttgga ctagtccaga ttgaattgct
    29161 tctgttagat tcgttagaat tggttttgtt tcttcatttg taatcagtgc aatcccttcg
    29221 agtgaaaagc gatttccaat acaagaagcg attcttagat aatattgaac atgatttgag
    29281 agcttctgaa tttttgatgt cattagctca acaacgttat ctgatatatt taatcctaaa
    29341 attctttgta aatcccattt ccaaggagta tagcctgatt tagaatcaaa atttggatcg
    29401 aagactagat actcttcatt atagagggac tttaaaaatt catttgtaaa gaaaggattt
    29461 ccgcctgttt tctcatgaat gagtgaagcg agatctctca cattcgccct ttccatacga
    29521 agtgtatctg agattaattc ctcaatgttt tctttcgaga gtggatttag ttttatctct
    29581 ggaatttcaa cgacaaaatc agagtattca tctttgaaac gatctttaat ttcctttaag
    29641 gtaattttca atggatgtga gtcgtttact tcattgtcac gatatgcgcc aatgataaaa
    29701 atacaaacct gttctcctaa acttaaactt tctaaaagtt tgagagaggc tgtatccgcc
    29761 cattgtaaat catctaaaaa gatcgcaagt ggatgttctg gctttgcaaa aacagagata
    29821 aatctttgaa aggtttcaaa gaatcgattc tgtgtttcac tcggaccaag ttctggaact
    29881 tctggctgct ctcctattac taattctaat tctttgatta catctgtgat aacagcaccg
    29941 ttatttccaa gctctcgaat gattttctgt ttccaagact ttagagcctc ttcgggttcg
    30001 ctaagtagtt gtcttactaa ttcctgaaat gcttttagta tagcaaagta gggaacattt
    30061 ttttgcagct gatcaaattt acctgcaata taatagcctc tttgttttgt gatagattta
    30121 aaaagttctt ggactaatac tgatttgccg attcctgaat aaccgcttac aagcataata
    30181 gggatttgtc ttgttgcaga aagtcctttt cttctaaagc tttcgaggta ttcttcaaag
    30241 tgcgttaaga gccattccac ttctttctct ctaccgtata acttttgtgg aatgttaaat
    30301 tcttggctta catcatattg tcccagagga aatagatcaa tttttgaatt tatttttact
    30361 tgttctaagc attgctctag gtcttttaca attccaatcg aactttgata tctatcttct
    30421 gcatttttgg caagtagctt tgcgatgatt ctagatactt gttctggaac gttaggattt
    30481 atacttgaaa cttgtttagg ttcctgtgtt ataattgcat ggataaattc taatgggtca
    30541 tcagaataaa aaggcatttc atttgtaagt agctcataga aactaacgcc taaagaataa
    30601 taatctgtgc gatagtcgag agatcgattc attctacctg tttgctctgg tgaaatgtaa
    30661 ggtaaagttc cctctaaaga gctaggattt ttaattacgg gattttcttt acttaaatga
    30721 gttgctattc caaagtcaat gatttgtatt tttccatctt gaacattgaa tgttatgttg
    30781 gatggattaa tatctttgtg gatgattctt gccgcatgaa tttgtcctag aacttgtgca
    30841 cattgaatcc caatttctaa aaattggatc agttctaatt ttgtgccgga gaaaatttta
    30901 cgaagagatt ctgcacctct gtcttctaaa actaatacga cagagttacc atctctttcg
    30961 agtgcatagg cttttacgac tccatcagtt ttgagttttg aaaggacttc atattcttgt
    31021 ttgaattgtt tgatttcttc tggactagga aaatcttgtg agatcttttt tatgatgact
    31081 ggagtattat cggaagtcct tttgcctcta aaaattcgag agtttacgct ttcatagatt
    31141 tgttcttcaa ctttaaatcc accaatttga atcatgtatt gccttcttat ctaaattgat
    31201 tttattacga aaaaaatata catctcttat atattaaata gaattccgta gttctaacat
    31261 tagggttcag aaaaataatt cagtcaagaa ttatatctat tttccaaaaa aatcagtaaa
    31321 cttttctaaa gattaaaaag aagaatgatt ctgaataaaa aaggagtaag tccagtttag
    31381 aacgataatt catctgtcta aaaattcttt ttctattgac aatacttata ttcaaaatca
    31441 tatatctgcg tatcaaaagg ctactaaaag gataaaacat gccagcttca aaggaaccag
    31501 tcaatttaca attcacagaa gagatgaaag gatatctaac tttttcggat aaacagttgg
    31561 attacaaaga aagttttgat tcaggaaaga aagaagataa ttatttcatg tttcatctaa
    31621 caattattat tcccgatgta gatttttttg taaatgatcc aaaggaaaca ggtgtagccg
    31681 agggttatgt ggaatcccca aagatgggtg gaagatttac agtagaaaaa ggtgtcttta
    31741 attgctttgt agatgtagat gctcctgtaa aaaatactaa gaatatgttt tatcgattat
    31801 tttttaagga taataatggt aagccactta caatgaccgg acacaaagtt gttagaaatg
    31861 acgctggaat tgatatatgg aaagatacaa cgacactcta tacaagaatc tatgaaggtc
    31921 atgtagaaga aaaggcagag aagacttcaa agcttgtcgc gaccggtata ttggaaatct
    31981 atgtgaaaga ttttgcaaag caaatgacta ctattaaatc gaacggtaag acttttgtag
    32041 aaagagagag agccgttatg caatttggta aactttttct tggaaatctt tgggatgtat
    32101 atcgacctta tattaaaaaa ggggaacctg aaatttggaa tcaaagagag attccaatgt
    32161 ttacttttga aggtgtaaaa gaaggaagta ttacaaatca tccaattacg acaggagaca
    32221 aactagggat tagcctcact cgtttcaaaa agaaagaatg tgatgatgtt gtgattttat
    32281 cacatggtct aacaacctct acggatatgt atatcatgcc agaacacaat aatctggtga
    32341 actatctaca tgaaaatggt ttttcggatg tatggtctct cgattggcga ggaagtatgc
    32401 gtcatagtta taatttattt ccacatagat ttaatttaga tgatgttgct ttgtatgata
    32461 ttccagcagc cattgcagag attcgaaaag agattggtcc aaagaaaaga attcatgcaa
    32521 ttgtgcattg tgtgggatct attacattta tgatgagtct ttttggaaag aaaatcgatg
    32581 ggatctcaag tgttatttca aatagtgttt ctcttaatcc aagagtagcg acttggtcaa
    32641 aaattaaact agcagttgct cctttcgcta ttgaatatct acttcgattt ccaaatgtaa
    32701 atccgcgttt ctcttatagt ccaggtcctg ctttagggaa ggggcttgca aagattgtaa
    32761 gtttatttca tcgagagtgc aataatcctg cttgtcatat gctatccatg atgtggggaa
    32821 ctggctttcc tgcttgttat gagcattcac agctctcacc cataacgcat gatcgagtgg
    32881 gtgatttatt tggagcaact tctatgaact atcatagaca tattcgcaga atggtaagta
    32941 aaggaaatgc tgtgaaatat caaattgaaa accctcgtta caatgaccta ccgaataatt
    33001 atttagatta tgcgtctgag atcaatacac caattctgtt tatgacaggg gataaaaata
    33061 gagtgtttaa tgattctaat attgtaaccc atgatacttt aaagaaaata aaaccggata
    33121 ataaaaatga actttttatt gctaagggtt atggtcatca agatactctc atgggtaaga
    33181 atgtagataa ggatattttt ccgcgctttt tagaattttt aaataaacat aaaactccaa
    33241 aaggttggtt ttaaataaaa actcttgtga ttttggaaga atatgagtga aagcttaggg
    33301 tagttcaaaa tcaagatgaa gattgcgatc attggagatg tgcatggttt ttggaatgaa
    33361 tttgatgtag agtatttcaa tcaatccgac tatgatttta ttttattcac aggggattta
    33421 agaagttata ccgaaaaaga atcagttgta gcagagagga tttctttttt agagaaacaa
    33481 gcttttgtaa ttccaggaaa tacagataca agcaattcat ttcaagcggc aggcgaaatc
    33541 tttcgaaatc aatttttaat tcagaatttt tttctagggc aagaatccag actagataag
    33601 atgaaagctg cccttggcaa actacgatta gtcggatacg aagtccatac actctctaag
    33661 gaaaaaagca ttgatctaat tgttcttaga ccttttgcta tggggaaaga actgagctat
    33721 gctcctattc taaaaaaaag atatgggatt cgttctatgg aagattcaaa agagcgcatc
    33781 aaaacactga ttgattcctc cttggaagag aatcttttat ttttaggaca caatggacca
    33841 aaaggcttag gagagaagga aactagtctt tggagttctg attttacgga gactccaatc
    33901 gattggggag atgaggacta tctttttgga attgaatatg cgaaaagtag aggaaaacgt
    33961 gtattagccg ccattgcagg acatatgcat catagattga aaaaaagaaa tattcttaga
    34021 aattggagag aggaaaaaga ggggacttac tttataaatg cggctagagt gccacgcatt
    34081 tttaaaatag attcaaatac cgttcatcat catatccgat tagaaattaa taaaggggat
    34141 gtctctgctc gggaaatgtt aatccacccc taaattattc ttttcgcttt cgattgtagt
    34201 taattttaat aagcaggtat aaggaaaggt aggctccaat aaaaactaca atcaaattaa
    34261 cattggaaag ctgaattgtt cccagttttg gagacatgag ccacataaat atatctcgta
    34321 taaaagactg taggcttgct aaaagaagaa tagaaccgag tgcagagata acaattgctc
    34381 caataaatcc accaatgatt tctttcttga gataaaaata aaaataccaa gctataagcg
    34441 aggatatggc tgtaataaaa ataatatcaa ctagtatcgt gagtggttct gaagtagttg
    34501 ctaaaaaata agtcataggg gaagaaaacc atttgtttaa attttgccaa gtatttcttt
    34561 aaactgcttt gatttagtta ttagcgaaag gatttgatat aatcggattt tcttttctga
    34621 attaattcct ttaggttcat ggtttccttt tggtttggtt ctaatttcat acaatattgc
    34681 agtatttctt ccgctcgctt gaaattgtta atcagggagt gacaatctgc tagagtatgt
    34741 aaatttttaa tgttctgtgg gtctctcatt cgcaatcgtt cgcctatctc tagtgcattt
    34801 tcgaaatcac caatcatttt ataaaaagtt gaaagcttat gtaaaaagtc taggtctcct
    34861 ggacaaaaat ctatataacg tccgcccgtg attgttgcca tttgaagatt atccaatcga
    34921 aaatagacat tagataaaat ttttagaaga ctatactcat ctgggtattc ttcaatttta
    34981 gaaattacaa attgaattgc ctcttcacct tttttatcaa gtaaaagttt ttccactatt
    35041 ctcgggatac tgtatatatc tggcaacgtc ttacgatcta gatttatttc tttggtatac
    35101 tcaattttca gaagagataa atcatccgta acctctccat aggttaataa ttttttataa
    35161 atttgaggca aatcgcaatc agtctcttca ataattttta aaattctaag aggatttgaa
    35221 ttcattcctg aaatagtttc atttgatatt cgtaaatcgt ctcttccatc agaacctatc
    35281 agtaatacat cgcctggata catttggaaa gtttgaataa aaactccagt gttgatttga
    35341 agggttccta attttctaaa gagaggttct ccttcaatgt attctgcttt gttgtctcta
    35401 aagagaatac tgggggggtg ttccgcgttt atataataaa caaaaccatt taattcatca
    35461 actagaccaa ctacgattga gacgagcatg gatccactga agctttcaaa gactctatgc
    35521 atttcaataa atgcttgttt tagccaattt tcaggagact gttcactggt tgttgcagat
    35581 aataacgttc tttctacaat ggattgaaag acagaaccaa gaatcaaaga tcctccagct
    35641 ccttgtatag atttacccat agcgtcgcca tttaaaaaga catgaaatgt tcttcctctt
    35701 agttcaattg tatgactaac gcatatatct ccaccaattt cattttcttt tccacggaac
    35761 tcaaatgtct ttttttgttt aacgaagaag gaaaatttaa aattattact ctcggcaaga
    35821 ttttgtccaa gaggtcttaa gagaaggcta gttagatagt aatctccatc ttgttgaatc
    35881 ttcatggtct ctacattctt gagagaataa tttaattcca ctgttctttc tttcacttta
    35941 ttttctaggt ttatattcaa atccgaaatt tcacgataga gtgaattgag ttgtttgatt
    36001 atctcattta aattataagc ctgtagtcca aaatcatctg aggaggtggg catactcaca
    36061 attgaaaaat tacctttccc taattgattc aagtgttcat tgattcccat gattccattt
    36121 ctaaatgttt ttgctactac atatcctaca atgattacag gaacaggaaa taataagctc
    36181 ataatcgtta gatgaagtcc tgggtcttct aatttaacta aacctatttt cataccgtag
    36241 agcatatatc ctaaaacgat aaccggcata acagtgattg aaatcatgga gagaataatt
    36301 cgttgaaaag aattaaactg tggaatttgg ttaattccaa tttcaatctc cttgactttt
    36361 tttaatttaa aaatagctcc aatcacattt tcactgataa aataataggc tgtataagag
    36421 attggtgtga tcattagaaa cagaaaaggt gccgttaggt gaagacctgg gcgaaatcct
    36481 gcggctagga ttacaaatag gtgagaccct atgataccgg tgagccatct aagcacaatg
    36541 atctttgctt cttgaaatgg ataatttaaa agttttagtt ttattttaac tttgtcttct
    36601 tttgaaatat ttttctcggt gtaaaagttt tttatttgtt taaaaatttt ctttagatag
    36661 aaaaaacgcc atacgcaacc tagaataaat aaggctgtgg cattcagaga tcctgcgaga
    36721 gctacaacca tcatctcatc tttagagaga taagaacctg ttcctgttac aactgaaaaa
    36781 tagatagcaa aaggaagagg cacaatatgg gtaaaggtct caagtcgcag tgttagcttt
    36841 atataaagtc ttttaaacat gcaagcaaca agtaaacatg acttttttga attgtcattt
    36901 cattatttta ttttttttgt aagcggatct gtattttgaa aaaaggaact aaatacggaa
    36961 ataccttcct cgatcgggtc aattttcaac ttatctcggt ctaaaatctc agtcatttgt
    37021 agaactaata gcccatacat ccatgcccac atggttcttg cgatcgagtt ataatgtggg
    37081 tttggtcttc ctcccgattt tttgatgagt ccgtcccgaa tggttcttaa aaagactcga
    37141 taactactgg gggctggaaa taattttttg tgcaaccccc caccagaact aaacatcaat
    37201 ttatgaagtc cttggttttc aactgagaat tgccaatagg tgtttgcaat tgcttttacc
    37261 tgctcaagtg ggttttccga cgaagcacga gccagtttta agtctgaggt taatttctgt
    37321 tctccgttaa atagaagagt ttgaatgatt tcttcctgac ttttgaaatg tgcatatgga
    37381 ctagctacac tgcaatttaa gagcttcgca atggatcgca tacttagaaa ctcaacgcca
    37441 tctttttgaa ttatatgaag ggctgcagaa agaatttcgg ctttgtttag actattcctg
    37501 attcttctag ctttcttttt aggttctgct tttacaattt tcatgggatg aggtgagatt
    37561 aaaaaaggtg tagcttaaag caagaaaaaa cgtttccagt agcttgaaaa cgatcgtttc
    37621 ttttcttttt ttatttataa ctaacagtaa ttaatatgaa ttgactttat ctattgctgt
    37681 tagttatatg gtttaactat ttttaaggag ttcgaaatgg tagaagcgtt agaaaaagag
    37741 gagatggtaa aagaaccaac tctttctgaa ataaaacaag tggtcagaga ggtttgcttt
    37801 aaaccaaatc gaaggaaagc aggtcttttt ttattctctg gagtgattca atttatattg
    37861 tcagtcagtt tttctatcta tgtttgggaa ataggagttt atgcgttatt tcctattgct
    37921 tggtttgtat ctggttttgt ttttacttct ctttttgttt tgggacatga ctgtgcacac
    37981 gaatcgttta ctggaagtaa aatgttaaac tcatttctag ggcacattct ttttattcca
    38041 actttttatc cctactatgc atggaagtat tcgcacgccg cacaccatca acacacaaat
    38101 gaattatatg cgccttccaa tactgtctat tttgacaatg cttggattcc tatgacaatc
    38161 aatgagtata aagttatgca gaaaaaatat cctgtcagag cgtttctcta taaactcact
    38221 cgaattgttt taccaattgg atcttttatt cataatatag tttatcatta ttttccaaat
    38281 aaatttaaac cagatcatag gaaaaatgtt tacttttctt attttgtttt gggatttgtt
    38341 ggagtctctg ttgcggttac tctttattat gcgacaggaa gttggtttgg tatctttcat
    38401 ttttggatta tacctgctct tgtttttcag gtttggatgt ctctttatac tttcttacat
    38461 cacacatcca gtgagatgca gttttaccct aaaaaagatt ggagtcaata caaaggtcaa
    38521 atcaaatcca ctaccaacta tcgtatgaat aaactgatat ccttccttca ttttcatatt
    38581 gattgtcata ttcctcacca tcttaatatt aaaattccaa gttatcattt acttgctgca
    38641 caaaaagatt tgtcgaattc aaaatattca gaagacattg tagaagaaaa atttacatgg
    38701 aagcattatt ttaaaacaat cagagagtgc aatctctggg atgaaaaaaa tagacgttat
    38761 gtgcgatttt gtgatgttta ataggttcac tcatctcttg cttatctaaa ggtttggtaa
    38821 ttcttagaaa aagacttgta ggtattttta ttatcacttg cttctagaat atttataaaa
    38881 atacctattg ttctatttaa aaatattgat ttaacttctg tctggtcagt agataaatat
    38941 tttagcaccg ataggcggaa tcaataaaga aaataggatc aaaatctcat atagaatcct
    39001 tttttattta cttattaaaa tctggtgcag aactaatcct gaataaaaag gagtaaattt
    39061 acttctattc tagttagaaa ataaatcaga ttttacataa ttttacgaaa ttgtgataca
    39121 aattggttta catttcaata taaatacaga tttgtcttat ctctcaattt atagagaatt
    39181 gggattaata aatatttaat ctttagttct gtaattctta gtaaaacact tgccaaaatt
    39241 agagtatttt atgtaaaatg caatggcatt tctcaatttt gacttagatt ttcaatctaa
    39301 attcattcat tgaatagcaa aatgcaatac aagaaagatt tccctacagt gtaattacat
    39361 gataggtatc atttgctctc tagcgtttga tgcctttttg aatagcaagg aagatttatg
    39421 accggtctta aaatactgac tcgacttctt ttctagttat caaagtaaat ttatagctcc
    39481 aatttaataa gtttggaggt ttttacacat tcgggcataa ttcttggctt tgaatccatt
    39541 ttttatctca gagcaatata aggtaaaagg tttatgctta aaagattaga agaattttta
    39601 gtaaagagga tcttaacata ccgcactgga tcggatcatt ttcgagttgc ccataatatt
    39661 ttttctttaa aattctttga gttaaattta gaaaaagaat ttttagattt taatttactc
    39721 aattccattt tccaagcaag aatcgctgcc tttctaggct tgctcctata tccttcattt
    39781 ttattagttg attttatttt atttaaaagg gaagattttg agttttctgt ttatttacgt
    39841 ttatcgatta ctatttttcc aattctattt tcttttttat attcgatttt tgctgattta
    39901 gaaaataaat ctacgattga ttggattgaa agaattgcca tctttacagt tctaagtgca
    39961 caattaggtc attgtgcttt gctttttttt acttcctctt ctccttatta tgatttagct
    40021 gttacaacga ttatattgtt ttttattttt ggtatcacca aaatacgatt tatatttaac
    40081 gtatacctct tactcctgac tgatttactc tttggagttt ctctttatgt ttctcaaaaa
    40141 tgcaataacg aagttttctt tgcgattcta tttatactaa cgtcattttc tatttgtgca
    40201 ctcctagttg gatacatagc agaatatcag aatagaagaa agtttattct tttaaaaaaa
    40261 ttaaatgaag aaaagcaaaa caacatcgaa acaaaaaatg aattagaaag tgtaaatctt
    40321 tctgagatac ttcaatttca acaaacaaat ttagactaca attccattga agggatcaaa
    40381 gactttcaat acgaaggaga gattgaactc gatcgatcta ggatgagacg agtgattggg
    40441 aatctaattc agaattcaat tgaagtgatg tatgacggga gaaaaccata ttatgtaaaa
    40501 gtattcagcc gagaggaagg agacacttgt gtgattggtg tagaggataa tggaccggga
    40561 ctacccgaaa gtttacaaaa gaaaatcttt gaagcctttg caacagaagg caaagcaaaa
    40621 ggaacaggac taggactctt tatgagcaaa tggatcgtag agtcgcatgg agggagtttg
    40681 acttatcaaa caacgtccgg agaaggaacc acgttttata taaaaatacc aaagaaaaat
    40741 aaaaaggaaa aacaatgaag aaaatattaa tagtggatga tcagccgtct atcgcgatgg
    40801 tcttaggaga tatattagaa gagggtgact ttcagattgt caatgcagtc aatggggaag
    40861 taggaattgc aaaagcaaaa tcagaaaagc ctgatttaat tatcatggat atcatgatgc
    40921 cagtaaaaga tggaatcact gcgatacgag aactcagagc aatggatgaa tttaaagagc
    40981 ttcctatttt tatattatct gccaaaggcg gcgcgcatga tgaaaaccta gtgcaagaat
    41041 taaaaatcag tgggtttatt cataaaccat tttctcctgg agtgatttta gaggagatcc
    41101 aaaaggcttt gtcatgaggc aaatcgttct tatcctcttc gcatttcatc ttagcttttg
    41161 tatgaaagaa aagatgaaaa taccaaaagt agaaaaagga gtattagatt tatcctcttt
    41221 agaatcagta gacgcaaagg attttatctc atccactccc atttcaatag atggagagtg
    41281 ggagttttat tggaaagaat tttacgcacc cagtgacttc ctagacccaa ataaacaaga
    41341 agagatagaa aagaaccgag aatacttaca atccaattct agatggaatg gttctatctc
    41401 gaacgcaaaa aaagaaacag caaaaggaga aggatttgcc acctatcgaa tgcgattaat
    41461 ccacactcca aaagagataa ctcatttaaa actttcgaat gtgtatacct cctatcgtat
    41521 ctatataaac ggagttttat tgcatgaatc aggactcgcc ggaaaagaca aagagagttc
    41581 caaggcttac gtgagagata aaattgtaaa gctaccagat tcaaaaacgg gtcagtttga
    41641 aattctcttt catgtttcta attattttca taggctaggc ggattggtat atccaatgtt
    41701 tctacagtct gagtctttta tttatgaaga gcaaaaaaaa gagtttgctc tcgctctttt
    41761 ttttacaggg tcctttttac ttatgagtat ttatcatttt ggtttgtatc tacttagaac
    41821 caaagattta tcccctcttt attttggaat cattacactc ttacttggga ttcgaccgct
    41881 tgtaacagaa aattttattt tggattattt cccttttttg gaatatgggc tagtaaaaaa
    41941 aatagaatat tttacaatct acttaacggc acccgtaatt ggtttgttct ttcaatctgt
    42001 gttttataaa actatgaatc gcaagatcat ctatggattt gtatttattt ctagtttatt
    42061 ttgttttgta gttctttttt tccctgtgcg tatcttctct gaaacactga tatcatttga
    42121 aattttagtt ttaatgtgtt cctgctttat tttgtattcc attctaaaag cagccttaca
    42181 aaatgaaata ggttcaaaga ctctattgat tggatttggt atatttttta tcactgttgt
    42241 aaatgatata ctacaccaac agggaattat ttatacaggc aatctagttc cctttggatt
    42301 ttatattttt attgtatccc aagcctatct actatcggtt cgtttttcaa atgcatatcg
    42361 agaagaagag agagcgaaag aagaagcgga gagacaaaag aaatatttag cagatgcaaa
    42421 aaaagaaatc gaaaatctaa gtaagacgaa agacgaattt ttggcgaacc tatctcacga
    42481 aatcaaaacc cctctctcga ttgtctatgc ctattctcaa atgcttcctg cgagtaagga
    42541 gaatccaaag aaaatagaaa agtatgcaga tcaaatctat tccaatgcaa gtaaattaaa
    42601 tgattatgta tctgatttat tactcgtaac agatatcgaa tccaatttag aattacaaaa
    42661 aagagaaacc gattttaacc aaatcttaaa agaaagtatg gatacctatc gcgatctagt
    42721 agaacaaaaa tcgattcaag ttgagtatca agaaaatgaa tcgatctatg taaattgtga
    42781 tgaattgtta ctcaagaaat caattcaagc agtgtataaa aatgcgatag tatataatag
    42841 gcatcatggc aaaatcaaaa tatttgtaga gaaaaaagac aacaagttag aactccaaat
    42901 cgaagataca ggaatcggaa tcgcaaagga atttcaagag agaatctttg agaagttttt
    42961 tagaatcgat agttctctga attatgagac aagcggtgta ggaattggat tatttttagc
    43021 aaagaaaatt ctagagatgc acttgggatc aattcatgta gaaagtgagt taggagtggg
    43081 cacaaagttt acaattcaaa ttccaatttt aaatacagta ggagtatcca atgtataagt
    43141 ggcttttctt gtttttctta atttttattt tcgattgtaa aaatccaaat gccataagtc
    43201 ctcccaaagc aataaaagga gagttggatt tatccaattg ggattttcat tcgcccgata
    43261 cagatggaat tgtgaagtta gatggggaat gggagtttta ttggaatgaa tttttggatc
    43321 cgactcttaa cgcaagcgat ccaattggaa atgattccat cgtgggtcgt catgaaaata
    43381 aatcttttat tcaagttcct ggagagtgga ataaatatgg ctatcctgag gagggatttg
    43441 tttcctataa attaaaaata cgtttgccgc aaggtaaaag gaaattttca ctctacctta
    43501 gaaatataag ttctaattat aatatgtata tcaatggaga attggttact gcagtaggaa
    43561 aagtgggaaa atcggaagaa gattcaagcc catatatggc atataaaaaa attctgattc
    43621 ccgaaacgat atctgatagt aataaattag aaattctttt tcatgtttcg aattttcatc
    43681 aatctggatc aggaatttgg catcctttat cgataggaac ttatgaaaac attgaagaaa
    43741 tttataagaa aaaaattgca ttagacttag ttgtaattgg ttttttggga atgatgggtt
    43801 tgtatcatct aggcctatat tctattcgta aaaaggatcg atcccccttc tttttaggct
    43861 tattttgttt gctcatcgta attcgtgttc ttactataaa cgaaagattg atctttgaac
    43921 tttttccttc tctgtctttt ctctttgtca gaaaaattga atatcttaca ttttattttg
    43981 gatcgtatct attctttcaa tttgtatatt ctctttattc ggagggtttc ataaagtcaa
    44041 tattttatat tgtcaatgaa atattcctgc ttgcaactct tacggttctt tttttcccaa
    44101 tgaaaatatt tatgtttcta ttgccgattc tccaaataat cgttttaatt gctattattc
    44161 aagttttaca tgcaacgata gttgcaattc gattgaaaaa aatgggagct ataatttcat
    44221 tattaggttt tattcttttt gccgttgcga ttattaatga tatgttaaaa ggacagggaa
    44281 ttatttctac accatctaca gtaagttatg ggttattggt ttttactttt tttcaggcaa
    44341 tcgtgctttc tcgcagattt gcagacggaa tggaaaaatc ggaaaaatat tcagaggaat
    44401 tacagaaaaa aacagatcga ttagaagaaa caactgtaga gctaaagcaa ctatcggata
    44461 atttggaaac taaagtagcg gaaagaacag acgctctgga aaaaacgaat aaagagatag
    44521 aaaaattaaa tcgaatcaca aagaaagtga attccgtttc tgatttaact gatattttaa
    44581 cagtcatctt attctacctg gaaaaggaat acaaattcaa tgaattttgg atgttattaa
    44641 aagaagagga agaaaataaa ctttctacat ttgcctatgt atcttctatc gcttcgagtg
    44701 atacaattac aaaaatgaaa aatttccaaa ttaatttaag tgaatcttct cttgttatag
    44761 aagcatacaa tttaaaaaaa gtagaaaaat ttttaatcca agaaaagaat ctttctactt
    44821 tagataagac atggattgag atcaatgcat ttacttattt tctcagtttg ccttttgttg
    44881 tatttgagga agtggtgggt attttactgg ttcatcatcc tagtataaaa gaaataaata
    44941 tagaacagat tccaaaacta caaagtttta cggatcaaat tgcgggagct atttcaaatt
    45001 ctagactttt caaaaaagca caattagcaa aggagcaagc agaacaggaa aaaggaatag
    45061 cgcttatcgc tcaattagaa actgaaaaac aaaaaaagga aatggctgaa ttaagtgaac
    45121 tgattaaaag tttaaatgaa gagttagata ttcaaaaaat tatgaataaa gtatatgaat
    45181 ttctgcaaag aaattttgga attcaatact atgctctcta tactgtaaat cctgagcaga
    45241 cttatatgct tccttttaat ataaaatttc cagaattcgt tacagaagag gaacagaata
    45301 aaatcctatc tatggttata ccaatcaatc aggatctagg ttctcatgcc tttgccttaa
    45361 gatttaaaaa gccattttat attaaatcag atcaagtgat caaaagattt gataagaatg
    45421 ttttggagga acaagaaatc attcgaataa ctcaaattaa ttcaatgctt ataattccat
    45481 tgattttaga taataaagct gtaggatttt tggatctttc taatagcaag aatccattga
    45541 atctttccaa agaagatatc acacgcttgt ccattttggg agaacaattg gctggaatcg
    45601 tgcacggatc taatttattc aaacaagtgc aagaagaaaa agaaaaagcg atagttgccc
    45661 agagggaact agtgcttgtg aacaaagaaa ttgaaaaact gaataagata atgaaaaaga
    45721 tcaattcagt ttctgacttg accgatatat taacgtttat tctttttcat ttagaaaaag
    45781 attttaaatt caatgaattt tggatgctat tgaaacaaga ggaaaaagag gaactttata
    45841 cattttcctt tgtttcccct tttgcgacac cagaaaaaat tctgtcgctc gaaaatataa
    45901 aaatcccaat gaatgctcca tcacttgttt gggatgcata tcaatccaaa aaaatcaatc
    45961 aagtcttttt agaagaaaag aatctttctg aactagatag aaaactaaaa gacattcata
    46021 acttttctta ctatctaagt ttaccgatgg tggtttatga tgaagttact gcaattctca
    46081 tggttcacaa ccaatctatt tcttctttta caaatacgca gatagaaaaa ttacaaaatt
    46141 ttacaaacca agtagcagga gcaatttcga attctaaact atttaaacaa gcgcaaatag
    46201 caaaagaaca agcagagcaa gcgagaggag aaatcgaaaa gctaaatgaa tttacaaaaa
    46261 agataaatga gaattttaat ttagatgaga ttctagattt agttggaaaa tacattttag
    46321 aaaatttcaa tattggtcat tacctattat ggaaaatgga tgcaaatcaa tctgaattat
    46381 ttccatacaa gggaaccttt catgaaagca ttcagaaaga aacaatcaat cagtttatga
    46441 aactaaaaat accaatgaac gaaaactctg gagtgcatgg agccgcttgt agaaataaaa
    46501 gatacttttt tgtaagagga atgggaagaa aaaaatcaga atcagaagca gaaaataaaa
    46561 ttcaggaatt actccaattt aaatcattac ttatagttcc tcttctgatt caaaacgaag
    46621 tgattggaac tatggatttt tctcattact cctctgaaat gaatttaagc aaagaagaga
    46681 tctctaagat ttgtgtcttt tgtgagcaaa tagcaggggt gctacatgga gctagtattt
    46741 taaatcaaat cgaagcagag cgtcaaaaat cagaaaaatt attattaaat attttaccaa
    46801 aagatgtagc aaaagagtta aaagaaaaag gatatgcaga gccgattcta tttgagaacg
    46861 ttagtgtaat gtttaccgac tttaaagggt ttacacaaat tgcagaaaaa ctttctccac
    46921 aggagctagt gaaagatcta gacgcttgct ttgttcagtt tgataaaatc agtgagcgtt
    46981 ttaatctaga gaaattaaag acaataggcg atagctatat gtgcgctggc ggaatcccta
    47041 ggcggaatac aactcatgct attgattgcg ttttagcagc acttgaaatt caatccttta
    47101 tgaatatgat gaaggaatta aaggctgcac agggttttcc ttattgggaa ttgcgtttgg
    47161 gaattcattc aggaccttta attgctgggg tgattggaga aaaaaagttt gcttatgatg
    47221 tctggggaga tacggtcaat tcagcgagtc gtatggaatc atctggaaca ccaggtaaaa
    47281 taaatatcag tgggactacc tatgaaatag tgaaagaatt ctttgactgt gaatatagag
    47341 gagaagtggc ggcaaagaat aaaggagtgg ttcagatgta ttatgtaaat gggttgaaga
    47401 gacagtattc taaagacgag gaaggaaaaa ttccaaatgg aaaattttgg gaattgtatt
    47461 caggtggatt gtaatggaat cttcattcga ttgtggtgaa taaataattt gcattttgtc
    47521 tttaagaaac tttatattaa tccaattagc atgaggataa gtttgggaaa atctttagag
    47581 taaataatta ttgttaagtg actttttttg attcttaaaa ttattatact acaattgaaa
    47641 ctgtgcaacc taacagggca acgaattcag ttaagaaaga gattcaaaga ttaccaccga
    47701 gaacaccgat aactaaacga taaaaaatcg attctaggtt tcatcggtgc tcatcgtccc
    47761 tatcggtggt attctttaaa aatagaaaaa ggagaagcat cattggatca gatagagttt
    47821 gcttttgaga acaaagaaat acttcacaaa aatgcaagca tagaagtctc gcgagtaaag
    47881 attcggggac aagaaaattc tgttatcgtg aagcgtatca tttctccctc tccagaagcc
    47941 tatgaatact atcgtttaaa aaacgagttt aaaatactga gtaaattccc tctttctaaa
    48001 agtgtaaaag taattcgact tgtcaatgag agaactttgg ttttagaaga tacaggtgga
    48061 atttctctta aaacctattt agtaaataca aatctaacta tagtagagtt tttagatctt
    48121 gcgattcgaa tggcagacgc tttagatgaa gtgcacaaaa aaaatatcat tcataaagat
    48181 atcaaacctt ctaatattat cattcatcct atcacaaaag aaattaggtt cactgatttt
    48241 ggaattgcaa cgttatttgc taaagaatac attccaagtc ttggaaaatt agaaggaagc
    48301 attgagtatg tatctcccga acaaacaggt agaacgggaa cagcgctcga ttataggaca
    48361 gatctttatt ctcttggaat aacattctac gaaatactaa cgggagaatt gccttttaaa
    48421 aataaagatc aaaatcaaat cttacactct catatcgcac aacaaccaaa accgttatct
    48481 gaaatctatc catctattcc aaattccatt tctgatattg tttctaaatt gatttccaaa
    48541 tctccagaag cccgttacca aagtgcagaa ggtttaaaat tagatttact tgaacttgct
    48601 tcggctattg gagagggacg gatcccgagc ggagtcgagg gaagcggtat cgagggaaaa
    48661 ttcttcgcgg gtaaaaatga tttttcagaa aaattttcca ttccccaaaa gttatacgga
    48721 agagatagag agattcaaag tttaaaaaat tcctttttaa atctattttc aaaagaacat
    48781 agaacagcaa ctttacaatt gatcaatgga tattcaggaa tcggaaaaac ttctcttgtg
    48841 caagaattga atcagccggt cgttgaaaaa aatggaattt tcatctcagg aaaatttgaa
    48901 caataccaaa aaaatgttcc tttctttgca gtcgtgcaag cttttcgaga attgactcgt
    48961 cagctactag caaaagataa aacagaatta ctcaattgga aaaatgaatt aaaagaggct
    49021 ctgggaaata tcggtcaagt gattttggat gtggttccag aagtggaatt gttcctaggt
    49081 aaacagccgc ctattccaga tgttccaccg gatcaagcct tcaatcgttt taagtatgcg
    49141 ttcagtcaat ttgtaagagt atttgcaaaa gaaaaacatc ctcttgtgct ttttctagat
    49201 gacttgcaat gggcggatat ggcatctctt tctcttttaa aaaatctttt aacggatacg
    49261 gatattatat atttctttgt agttggcgcc tatcgtgaca atgaagtaga ctcttctcat
    49321 tctctcactt tcttcttaca tgatttagaa aaaaacgggc taaaaataga gaatctgcaa
    49381 ctcggaaatt tagataaaga ttctgtttta tctcttgtgt ccgattctct tctttctaaa
    49441 aaagaggatt ctttagaatt aacagaactc ttattagaaa aaacagatgg gaatcccttt
    49501 tttgtgaggc aatttttaca atctctctat gaagaaaatt taatcaatat ctcagaaaga
    49561 aaatggaaat ggaatatttc tgagattcga aataagtctg tgtcggataa cgtattagat
    49621 ttaatgcttc agaagataga aaaattgaca gaaccttcgc aactcctttt aggctatgcg
    49681 tcttgtattg gaaatcaatt tgatttaaaa gttttaagtt ttctcttaca aaaaactacc
    49741 cccgatgtga tttctatttt agaagaatgt atcaaggcag gactcatcat tccactaagc
    49801 gatagttata aatggataga gtctgattcc agtgataact gctcttttcg atttttgcat
    49861 gatagagtgc aacaggcatc ctacaggctt gtaaaagaag aggaaaaagc aaagattcat
    49921 tttataattg gaaagatttt actcgaacat actgcaaaag aggaactaca agaaaaatta
    49981 tttgatatag tagatcaatt gaattatgga ctcgatttga ttcagacaga agaggaaaaa
    50041 attcagattg tagaattgaa tattcaggct ggtaagcaag caaaggcatc tgtagcgtat
    50101 aacggtgcat tgggttattt taagaacgct gaaaagcttt tcagtaaaga tgcatgggat
    50161 acaaattatt ctcttatcta tcaatgtgtt acagagttag gagaaacaga attcttgtgt
    50221 ggaaactttg aagaggcaga tataactttt gagattggtc ttgcaaatgc aaagagtcga
    50281 ctcgataaag caggaattta caatctaaaa gttgtattgt atacaggaca gggaaaatac
    50341 aatcaggcaa tcgagacagg gcttttaggt cttgagctct atgagtttaa aactccagag
    50401 aattttggag tcacaaatgt ttttaaagag atcaaggcag ttaaagcttt gatcggtgat
    50461 aatcctgtaa atagaattaa gacgttaccc gaattagtag ataaagacaa attgatgcat
    50521 cttcatttat tgatcaatct agtcgattcc acttatttta ctgataaaat tttgaatgga
    50581 tatattgttt tacagtcgat tcagttttct ttaatcaacg gagtctctct tgcgggatat
    50641 atttcctttg taagttttgg aaatttactt tgtgccaata gtgattatca attcggattt
    50701 gagataggaa aatatatcaa tgagataacg tcttctaata atctcacact cattaaaagt
    50761 aaaagtgata atattttttg tgcgcatatc aatcactgga aaagacatat caagactggt
    50821 tttgaatttc aattggaagg atttaaagcg ggaattgaaa cgggagatct tagttacgct
    50881 ggttataatg tggcgtggta tttatggtat cgattttttc atggagcgaa tttagaaggc
    50941 attcaaaaag aaaatttgaa gtttctggaa tttgctttga aagtaaaaaa taaaaatcta
    51001 gaacttctga ttcgaattat cgttcaaacg attcaaattt tacgagaaga agatataaaa
    51061 gaaaaggatt ctaatttgtt tgaagaatgt agaaagagtg agatgaaaat ccctctcttt
    51121 ggatatcttc ttttggactt agaattgaat tatcatttag aaagatttga aacaagtcct
    51181 tctattatta aacaaagttt cgaacatcag gattctgtat ttggtttgat gttctattta
    51241 gagtttcatt tttattatgc acttacactc attgcaatgc atttagaagc aggaacagaa
    51301 caagcacaaa atgagaaagc aattctgcaa gaaattaaat tatggaaacg atgggcggaa
    51361 gcctgtcctg aaaattattt gcataaatct ctactgattc aagcagaatg ggcgagagta
    51421 aaaggggaag tcgtttctgc catggattta tatgaagaag cgattgaatc tgccaagaaa
    51481 aatgattaca ttttaaatca tgcgttagct tctaagcttg ctggaaaatt ctatctctct
    51541 ttgaataaac ccaaatttgc ttatctattt tttatggatg cgtttttcgc ctataagaac
    51601 caaggggcag atttatatgc tagggattta gaagaaaagt atttgaaaaa acactttcag
    51661 tatatgggta aagaaagaag acaacaaaaa gtttcagact ctacttcgac cactcttgca
    51721 agtatcgact taaattctct attaaaagca tctcgtgtga tttcaagtga ggtcaagcta
    51781 gaacttctat tgaaaaattt gctctatatc attattgaaa atgcaggagc agaaagagga
    51841 gtattgcttt tagaagagaa gggggattat cttatttttg cagaatccaa agccaattca
    51901 gaaacaaatc tagaaaagag acaacaggat aactctcatt tgcctctctc tctcattcaa
    51961 ttggtgctac atacaaagga atatgtcatt ttacaccaag caaaagaaga taaacaattt
    52021 tcaaaagatg tttatattgc aaaaaatcca attctatctg tagcttgctt tccgatttta
    52081 aatcaaaacc aactcattgc cattttgtat ctagaaaata atcttcttcc aaatgcattt
    52141 actccagaga gagtagagat actaaatgtg ctttgttctc aagcggcggt ctcgattcaa
    52201 aatgcagagg tctatgaaaa tttagaggaa attgttagag tcagaacaca ggaattaaag
    52261 acgttattcg aagaagtaaa agaattaaaa gtcgcacaag atgcagacta ttatttaact
    52321 tctgttttac tcagtccatt gcaagtaaat aacaacacag cagaaacagt tcatacagaa
    52381 tttatcgtgg agcaaaagaa gaaagttaaa tacagagcgt tacgcgaaga gtgtgaaatt
    52441 ggaggagatc tttgtattac agatacaatt gaattgaatg gaagaaccta tacagttttt
    52501 ttaaacgggg atgcaatggg taaatcgatg caaggagcag gaggcgcatt gatcgcggga
    52561 gtggtcttta agtctggaat cactcgcact aaaaatgaga atacaaatag cctaacgcct
    52621 gaggaatgga ttcaggagag atactgggat ttacaaaaag tatttttatc tttcgatgga
    52681 attatgctcg tttcgatttg tatgggactt attgatacgg aagaaggaac tctctatttt
    52741 ttaaatgcag aacaccaagc gcctgtgctt tataaagatg gggttgccag ttatgttgag
    52801 catccgatcc aacttgcaaa aattggatct atattaaatg aaaagaaatt cttcttagat
    52861 gtaatgaaac ttacaagtgg agatcaaatt tttataggct ccgatggaaa agatgatata
    52921 aagattatag atcctgaaac gggagaagaa actatacaat cagatccaga tcaatttctt
    52981 caaattgtga atcaaaccaa gggtgatttg aaagaaatgg tttcagaaat aaaaaaatca
    53041 ggaactttaa tcgatgatct ttcgattcta aagatttctt atttgccata ggaaataaaa
    53101 tacaaaaata aaattgcatt gttgtagcaa aacaaaaaac tgatttatat acggagtata
    53161 aaatgaatcc ttttaaagta gagaaccaag aactaaaagg cggaaagact gccttcattg
    53221 gaattcaaac gagtgagcaa aacacagtca ctagagatca gattttagaa ttacaaagaa
    53281 tcttaacaga tttagataaa gattctacag ttcgaggaat tgtaattcac tcagagaatg
    53341 aaaagttttt ctgcaatgga atggatgcaa agcatatttc cacaactcca aaagatagac
    53401 ttgcagaaga gatgggcgag attataaaat tttttgtatt tatgagtgag atttataaac
    53461 cgttagtcgc tgagatttct ggttacgcaa tgggaggagg agctgttcta tcccttgctt
    53521 gtgattatag aatgatgcta actggaaaag ctagaatttc ttttacagaa gttttcttcg
    53581 ggcttccact tcctggagtt ttaatagaaa aatgtaaaat ggcagttctt ccgcaacatg
    53641 taaatgacgt aatctatgga accaattaca aagcagaaga agcaaaagaa attggtttta
    53701 ttcatgaaat tgctgataat aaagaagacc tacggaaact aacggttcga aaattggaaa
    53761 atttatacaa gtttcctctc tctgctttcc aaaaaacaaa agagtccctg cattatcatc
    53821 tctctcaata tgcagatgca cacatgaaag cactcgatgc aaatttccaa aatccagtga
    53881 ttcaaaagaa tttaatggaa gcaatgaacg cattccaaga gaaacgaaga cctaattttt
    53941 cttgagaaga gtttttcacc accgatgagc accgatcgct tcgcggcacc gatgggatta
    54001 agtttttttc ctatttattg tgtttgagca tgcgacaggc gctcaatacg gtcaacttaa
    54061 gagaaatgaa aaagaattaa ccacgaaggg cacagagaac acgaagaaaa gagaagattt
    54121 attatttatt cacttcgtga gtcttcgtgg ttaaaaatct ttaagttgac aacattaaca
    54181 ggcgccgtgc aagtcggtgg caatctttgt tatatcgata ttaggattat tttttttcta
    54241 gacttgaaac taactctgct aattcttctt ttaatgattt gcattcctta atctagtttt
    54301 tctttttttt taaagcattc acttgattca cagaatcttt taaatctttg ataaattcgg
    54361 cttttttatc agtgatttta gtcgttttca caaaggaaaa attttgttag acttcaatga
    54421 tgaaatctgc ttttttatct ggcacgtcaa ttaacacttt cataatctca ttttaagtca
    54481 aaagcgattt caatcaagcc ttttctttct agcccgatcg ctcatttgca tccaacttct
    54541 tccataatcg ttttctattt ccatcttact agtgaatggc ttaaagctaa aaaggattgc
    54601 gagtcttttc taaaaagatg ttttcaaact aaacagggga aatttttatt ttaaccatct
    54661 gatccttctt catttgagga acagagacta tatacagtgc ctaaattaaa tattatactt
    54721 tcatttctct ttgacaaaca ttcgtctaaa gattttgcat ttataccctt tacataatca
    54781 ggattattac aactaaagcg atagtcattt gaatataata aataatacag tgtggtttcg
    54841 cgaatgcagt gttttttctt ttttttagtt tttgatccag tcgggtaatc tggattttcg
    54901 caatttgaaa atagaactat aagagtaata aaaactaatt tcattctgct catttacact
    54961 tttgatttat acatgctctt atgaatgaag cactctcaaa gcggataaac aagattatcc
    55021 acagtagtag taatagaatt atttttttca tgttgttatt cctccaattt gttaaaggta
    55081 agatttctta gttttaaatt tcattcgaat agaggttgta ttaaatgtct tctttgcata
    55141 agacttctcc tgctgaattc ttgtcactat ctctaaaatt atataaagct cttgcatata
    55201 acgctttatt ttcacttata ctttccatta gccatgtaac tgcaatataa ttagaccctg
    55261 tattataaaa acctcctgat aattccgaca atagagtgcc taaagaatca tctttactag
    55321 gcaatttcca cttcttccca gcaaaagtat ccgttgcaca agctttcgcg gcggggctaa
    55381 tagcgggatc tgctttgccg tctttttcac agcttcgatc gttagtcggg caccattgca
    55441 attgacttgc tccccaaact gtatcttttg ttccggttcc ttggcaatcg ttcggtgcag
    55501 atcgatacac ttgaccaatc aaacaacggc gaattaggat tgtcttctgg ctaatgactt
    55561 tattccc
//
LOCUS       DAOQOS010000019        55323 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_218_length_55323_cov_5.420569, whole genome shotgun sequence.
ACCESSION   DAOQOS010000019 DAOQOS010000000
VERSION     DAOQOS010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 55323)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 55323)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55323
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_218_length_55323_cov_5.420569"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..209)
                     /locus_tag="PLF66_06015"
     CDS             complement(<1..209)
                     /locus_tag="PLF66_06015"
                     /inference="COORDINATES: protein motif:HMM:NF026420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transmembrane 220 family protein"
                     /protein_id="HMZ63632.1"
                     /translation="MQAKKEEVLIKVRIINFILFILFTLFALVQYNDPDPYIWIMIYS
                     YTAILCLLASFRKYYKYLYIAGAVIS"
     gene            complement(178..1170)
                     /locus_tag="PLF66_06020"
     CDS             complement(178..1170)
                     /locus_tag="PLF66_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016544925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HMZ63633.1"
                     /translation="MGAPSKEWKEEIASDEEVRFNGYAKSIEEIQKKKSAQYGTGRAL
                     HRKIICGLKAKLEILGNIPDYAKVGLFAKAQTFDALIRISNGGVDVKPDSVPDIRGFA
                     LKVLGQNGPNALGSGNTDSQDFTLINQSAFAFPKSAPFIALVIAASESPFSLIKHLFG
                     TYGVIGGFQRVVKTAKTFGKPFSGFMTETFYSAAPIACGDYAGRVRLLPVSNQKITSS
                     NNSDLSIDIKKRLATEDVEFYLQMQFFVNEKITPIEDASVDWLESESPYITLGKLTIP
                     KQNPDSEEGKALNEKIEKSVFDPWNALKVHRPLGDVMRARKVIYYASQKGRGAY"
     gene            complement(1475..2182)
                     /locus_tag="PLF66_06025"
     CDS             complement(1475..2182)
                     /locus_tag="PLF66_06025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63634.1"
                     /translation="MNQIIILIIFLFLFINSCSNLDGIQNKKDTRNRNNIFSLYLIQQ
                     RSLGNCLRKDNTNNIAYCDRRSKGLCNPNDLILTNGEKTYNLSDANDLLKAVPSCNFS
                     FLNSGISSDTITNQTDTDSLIANNTYSTVYSCESLGFSNAANLISELELLFLNSARGR
                     IAIAADKIVNTPDLLLAATLPSTTTASQVKADAQTCLNEGFPQSQKDLVSDIRSVKRV
                     KSVSCNLKTGATNSCTF"
     gene            complement(2182..4833)
                     /locus_tag="PLF66_06030"
     CDS             complement(2182..4833)
                     /locus_tag="PLF66_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012390274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HMZ63635.1"
                     /translation="MLRLFLILLVFTNSIFSQEKPISAKIKIIDKSTNSPKANATIVI
                     KESGGYFITNANGEVDFQVPANGYYTLRIISGTDVEVKRKEIYYDGQELFFFVGEKEK
                     GAIEVAGEKEKTKLSRYTLQQEEIKRLPGAQGDSLKAILTLPGVSPAIPIGLSTTAAL
                     IPTINGSPYSNSDRGDVVLRGAGARANQFYFDGFPVSYPFHLGNQSSVFNNNIIKSFD
                     IYTGAYSTRYGYATGGIINIEGKSDVKKTSAIINMNFFLSDALIETKLSKKSYMIAAA
                     RKNYPNLLLLRFYPDAIPPNAKYADYQDYQFKYGHDFSDKHKITFSLFGVRDRQAYTN
                     AQAEFESAGSGGGGSDNRPPIGLDKSFYTEGFRYIYQPTSKFSNTLSISRNTFKEFFE
                     VKITNPATAENIFGIQNVTTQTLYYVENIQSLELIKNILKFETGQNYRERNITLKGEN
                     ITQQNNQFTKVFNDLLDSSPTFRALIDGDGAKAKEYGAYGEATLDYAGFKLVPGLRWD
                     YYNLANQKALSPRVNTSYTIKKTGTTFIAGAGIHRNSPVGIEQVSSKVGNSKLLMEKA
                     EHMALGVNQEIGQQWLFKVEGFRNIFSDLVVSDGFARNPYAFNNEPRDIIQKPDFVSR
                     NILTDKALNYSNRGDGFSQGVEIYLKKSNKPGENGFFGWISYSNSITKRNNHQTRLAS
                     SEVTNRNLRNNTRKLLYQTEVGDNYVNYYDDNQLELLFDNDRRELYDLDRTHLLNIVF
                     GWKISSEWQLGGRFRYYTNTPITPIVGSTRIAQAASFGINLNNPLYSNDYNSARLASF
                     QQVDIRLDRFMNYEWGLVNGYIEIINLLGRRNISGQNFENTKPYLNGVNPSPVYDTLN
                     SPYVQSPLPGGRLIYLPLINFGIEVRF"
     gene            complement(4836..5099)
                     /locus_tag="PLF66_06035"
     CDS             complement(4836..5099)
                     /locus_tag="PLF66_06035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63636.1"
                     /translation="MAKLIRFSIFILFCLNCYDKNRIDSWEIQKRVALLIESKASQCG
                     NRPKEPLLFINDRPPGEVNQCELDLVNARCPFKTYPWSCVRIF"
     gene            complement(5083..6933)
                     /locus_tag="PLF66_06040"
     CDS             complement(5083..6933)
                     /locus_tag="PLF66_06040"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5939 domain-containing protein"
                     /protein_id="HMZ63637.1"
                     /translation="MSLTLEEFLKLYPWEEKYTANKPNRMDWFWNFELEIEPNSLWPF
                     ISDTSRLNQAMGLSKMTFEEKEGILYGKAKNAGIDLEWIEVPWNWVSGKYLVSIREYS
                     KGFAYTVKAIFHLEKINDKTTKLYVYLGWIPRGFFYKLLLSASKKIIFNKYKTALNEI
                     VKLSKNKKALSLLTSSETYYKIENKTLFERVKKQLLEKNLNIDIIKKLLEFIETGDDQ
                     DLYRIRVLELANKWKVSERELLIICMHATRLGLLTISWDIICPHCRGTRQEAVTLFEV
                     PAKGSCEVCEIDFDNNSENSIEITFHIHPSIRNVPKVFFCSAEPAKKTHIKMQKVLGP
                     KQFIEENLNLNVGSYRLRIKGERNFNILNITDTEEKKLINWFSADNTNFLSGTNPLIR
                     IENPKEKINLYTIEELAWDPFVLKPAYIFTLQEFHDLFSAEAISSELKLELGLQTVFF
                     TDIVGSSSLYDIQGDSKTFVQVKRHFEEIHKIVQENQGAIIKTIGDAVMASFPSPGNG
                     IQAANLLIHKFDGKNLDGIRLRISIHYGQCIAVNLNSGIDYFGKTVNIAAKIQKLAGA
                     SQIVFTKQYKENPDVIKFLESNKIRMEEIQYEIPGMKDQFTIYRIDGKIN"
     gene            7057..7389
                     /locus_tag="PLF66_06045"
     CDS             7057..7389
                     /locus_tag="PLF66_06045"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HMZ63638.1"
                     /translation="MKLEKSITNNRAIVEIEGYLDMGTFRDFEMFVQATSNEEKVANI
                     GLNLSKCRAIDSSGMGCILRIMNNLKGKNKKLFLLDVPSNIKGILKLSQVDKYLKILS
                     SEEFSKET"
     gene            complement(7474..8328)
                     /locus_tag="PLF66_06050"
     CDS             complement(7474..8328)
                     /locus_tag="PLF66_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015680115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HMZ63639.1"
                     /translation="MNNYLNLILRSIIESITEFLPISSTGHLFLFTSIFPFEGLEDGA
                     SFDDLFDIFIQSGAILSVLVLYFRYILDKAKQGIQYASGAIKDSSGLVFFINVAIGSF
                     PVLIIGFLFKSFLDKIKASEVLLLILGFAWLTGGIAIVIVERLLSKNNNEKENEIIGL
                     TFRQSITIGIFQCLALIPGMSRSAMTIITARVLGLPKKTSAEYSFFLAIPVLIAASLY
                     KLYKYRSLLHGDKLILLLLGFFLTFIFCMFIIRWFLAFIQKNSFSSFGYYRIILGITV
                     ISYFFIFK"
     gene            complement(8460..9215)
                     /locus_tag="PLF66_06055"
     CDS             complement(8460..9215)
                     /locus_tag="PLF66_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020781528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein LipL31"
                     /protein_id="HMZ63640.1"
                     /translation="MKSKLILINLVILSLFAFGGCDDDNSSVIETLDGEKLTVKKFET
                     TYETAIESMSRMQNVEKENVIKIISSDIEELDEQLKGINYQFQPKNFYENYRNMVMIK
                     TAADKAGFSTRPDIKNIVKYLEMQTISQLYITEQVEKKIKITDEDANNKCKELREKDK
                     RMQNVTLDRCVQIGRGVLKQQESEKILPKVIERVKEGISIKHNEKFDLEQYLKTGPSF
                     LKKDLKEKDSDKTESKSTPDSKTEEKPANSENK"
     gene            complement(9278..12697)
                     /gene="mfd"
                     /locus_tag="PLF66_06060"
     CDS             complement(9278..12697)
                     /gene="mfd"
                     /locus_tag="PLF66_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016544536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HMZ63641.1"
                     /translation="MKDILSLYVKKISETNFETIYSKKGQFRFHGIPDSASSFLASVL
                     YLSQNKKSAVVISATNSDSEALYREALSFLPSKSIAYLPGPEVIPYDYAKYSREIKID
                     RINTLARILNEERLLLFTSTIGFLKTLPSPKILMGKSINLKKGDDFDQTKLISSLVAL
                     GYSREEICDEYGKFSVKGGVIDVYSPLSSDPVRLDFFGDTLESIKTFDSTTQRSLSEL
                     EDVSILPVDEFLLSKEEWELYQREIEVRGKNLKKPDMEMAHHNALEELVPVVHTPHGI
                     LDYFKTKPLIIYHRQNEVVDKTFQIEREYRSLYEKRKTDIICLEPEKIISLEKEYSLL
                     IAKKEISFHLIKPSELKDTEAILPILETDSFKGKIRDVREKIETMKDGILVITSSFHA
                     QTERLKGLFEKENIQLLNESSEEPEIIPFPKKSGAYLVLSELKNGFILPEENIHIWTE
                     NDIFGRSYKRKSRFKKTSSRAIQSFIDLKEGDYVVHINHGIGKFIKIEKVTADSKIRD
                     FLKLEYAGGDTLFVPLDQISLVQRYVGGSEKPVLDSLGKSTWKKKKERAQGIIDVLAE
                     ELLVMYSNRMKLQGYSFPRDTIWQEEFEAEFEYEETPDQLSAIEAVKADMESPRPMDR
                     LICGDVGYGKTEVAIRAAFKAVMAGKQVLFISPTTILALQHFNTFTNRFENYPVKIGM
                     VSRFRSSSEIKNAIAQFSRGELDLLIGTHALLSGNVKPKNLGLLIIDEEQRFGVNHKE
                     AIKKIKNLVDVLTLSATPIPRTLHMSLTGIRDLSIIETPPKNRQSVETFVLEDNEEIM
                     KLAIEKELERGGQVFYLHNRVETIENEAKELGKILPNISIGVLHGQLNEEEVEETILD
                     FNNRKYDLLVTTTIIESGIDMPNVNTMIVRRADTFGLSQLYQIRGRVGRSGRKAYAYL
                     FYPENRALTEQAEKRLNTISEYQELGAGFKVAMRDLEIRGAGNLLGTEQSGSIMEIGF
                     DLYVQMLNEAVSKLKKEEIPVEVRTVVNLASNFYIPEDYIPDTKQKIEFYKRFEGALH
                     LDEMDEIAKDMEDRFGKFPEVVETFLQLEKIRVLGSGLGFEFITEDNEEIKLKSGQHF
                     KGDTNKIMKLISSREGLSIQPKEPSVLRYKPKNKKEKQRLTEIFQLLQKIS"
     gene            12798..13454
                     /locus_tag="PLF66_06065"
     CDS             12798..13454
                     /locus_tag="PLF66_06065"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HMZ63642.1"
                     /translation="MFIWVTQGIVEPYIPKVRPDTVKKINPTHRGIPDKSIEVGDPED
                     TIVISHNKTGFASDAYIDVADIYSQEQSILKEERKQIFYAKDIMTKKVKCIAPDDLVT
                     KVEEIFQEDRFKHMPVVDSNQFIQGILSERDLLKFTIELLRKTIENNQAKVQDIMQRK
                     VLSAYPETPIREIARIMFEEKVGSVPILDKEKKHLVGIVTRNDILRKVMNNPPLELYT
                     "
     gene            complement(13483..14115)
                     /locus_tag="PLF66_06070"
     CDS             complement(13483..14115)
                     /locus_tag="PLF66_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020777801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63643.1"
                     /translation="MSIDTLSIVNNDQRVFALRQFIEEHPYFELQDLYKWLYYGEFGY
                     EEHISYLKKEKGHQELHKILEDIKYEQNMENLSDLIWKPMGLSQRFVMVFVTQYYKEL
                     CPLNRLVNLIERSPAFQGTRMHFKLDWSFVKDYVIRTQTHRFTKQDFYGFEDRINFHS
                     LPLVSFTGTFMNKNPERYRVVPRKLFFDFFPEFNDNHDILPTRAGDSLID"
     gene            complement(14105..14725)
                     /gene="plsY"
                     /locus_tag="PLF66_06075"
     CDS             complement(14105..14725)
                     /gene="plsY"
                     /locus_tag="PLF66_06075"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HMZ63644.1"
                     /translation="MEFIFLFISFLLGSFPSAHIVSKMQGIDIKKMGSGNPGATNVLR
                     SVGWKSGFIVLIIDVGKGILPFFLFPLFSIESAFLEKEWILLLGGVFSILGHIFSPFL
                     GFQGGKGIATSIGIYLYLLPIPCLMSAVLGLIVVYWKKIASLGSIAGFFFLPIFYRLY
                     EGENYSSKLFILVVFNFFLVLFTHHENIKRIIDGTELKFKNKTNVN"
     gene            complement(14725..16083)
                     /gene="der"
                     /locus_tag="PLF66_06080"
     CDS             complement(14725..16083)
                     /gene="der"
                     /locus_tag="PLF66_06080"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010418219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HMZ63645.1"
                     /translation="MKKLPVVSIVGRQNVGKSTLFNCFLKQKVAITHDFPGVTRDVLS
                     HDIENENFIKPFTLSDTPGLDIENINDLTSSIIQISFEHLLESDIIVYVIDRNEILDY
                     DSKLIKLFLTDKRFVKKNIICCVNKSDNPDNDFDLEYFYRLGLQEVIPISALGRRNLK
                     ILFDKINFYLAKVRMGEKQNTDFSICIVGKPNAGKSSFLNAILGYERAVVSEIPGTTR
                     DTVHSVLKFEDMNILIVDTAGIRKNSKSSEELEFYSYKRTLQSIEDSDIVVHIIDASK
                     GLGEYDKKIFAIIRKSGKPVVLAINKWDLIEDKDSNTFSEYKKDLISRFHPTAALPVI
                     SISAQKKQRIRKVLEECVKIHKKVNTKISTSKINQKLSEWMSEGKMGLQSKKLPRILY
                     ATQVSTTPFKLLLFVNHVDLFKKPLLSFIKNRITEEFQLNGVQVELEIRSDRKKEKDY
                     RE"
     gene            complement(16080..16568)
                     /gene="smpB"
                     /locus_tag="PLF66_06085"
     CDS             complement(16080..16568)
                     /gene="smpB"
                     /locus_tag="PLF66_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016549013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein"
                     /protein_id="HMZ63646.1"
                     /translation="MKQKEKEKETGYKEIVVNKKARFDFELLEFLEVGIVLSGSEVKS
                     LREKRANLTDAFAQVKNGELYLQNFHITPYKNGGYANHPEVRPRKLLAHRKEIIRLEK
                     QIKEKGLSLVGVKIYFNEKQFVKLQLAIAKPKKLYDKRESVQKKEAKIEIDRALKNRN
                     RS"
     gene            complement(16571..19582)
                     /locus_tag="PLF66_06090"
     CDS             complement(16571..19582)
                     /locus_tag="PLF66_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008592278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HMZ63647.1"
                     /translation="MESTKIAGIDVPVYKNTNPSLVYPSNLVETDTTLKNLRNILFPI
                     LEGQPVLLVGDAGVGKNALIYYINSKRNHPTLRYSFNEDTLPEDLIGSYRLLMSGAGF
                     EWIDGPIVNAISNGYTFVADEMNLCSPNVIKRFATVYESAYIDLMEGDGSRISAKEGF
                     GFIGTQNPSEGFEGRKPLPFDITKNFSTVFIDPYPPEEILFILKKLYPELAESLLQSC
                     IRITLSTEKKVLNNEIGKGDLEKYHFNIRTLKKICERLLAFNCNDKSIVYRELYNLFI
                     EPFRKAEDKKSQEELVSQELQYSVKETKQEIKLYVEKDKLYCNDKSIHTDETISKKIL
                     SEIPITEKTLEFIEKVITAIQLNENILIEFGEEDDPNFLMSLITQISGLKLSHVNLCK
                     GIHTSDILGALKPVSGSKVEWIDGPLTRGIREGGTIVITALEAAGAELVEKLNMLTDD
                     AKAITLPPESGEERPLHLKEDSRVFAFKMFRKTKSIPTISRAFRNRFTPILFPNLEDE
                     KTLKEILQFYIVDETLADIMSAFHRKIKTLSEKRVIGSGNLNPYLFGLSNLLKWKDHI
                     LKYNHPENLSEVVVRGAKISYINQIADPKERFDMERIIDTAVKAKKFPEDLYQKIEEK
                     KKTFTEPVNLDRNPWWDPNLHKREANTGKAPLKNSGSPLKKGIDINTPETGGNTKEGA
                     DAWYGKETQGNMGQGEPAGGGGAWGYRTEELYKQFLAKRKILWNYSIAVSLKEFKEVF
                     EKSLEEVEMNLESLFDPEIDITRMYQTQGKRVDTRKYISFKSGRGDSKVFDKTIIDKN
                     EEKLKGVEIVFLVSKARRIFNFEYSVATLSAMLTSAYILNEHNVKFSIYTYSDRLNRK
                     DHIDLICLKDKEEDYDTRKEEEMFNSLTKDWQGDSVYEFSLIEDCERYFTPEAETRII
                     VIISDFRGQRGKTEIDREIESWENKKLKEEVLKNVKKNYVFLAVGLGNRYIAENLFEN
                     SVQITPDNFSNMPNLIGTELTRLIHVHHAVR"
     gene            19678..20079
                     /locus_tag="PLF66_06095"
     CDS             19678..20079
                     /locus_tag="PLF66_06095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63648.1"
                     /translation="MDLMKHSLTKTLFLPIFLFILFCDLENRYPDAGEFCTPMKETPD
                     CVKMDFRKKELELQNPNRILKLNMITRVEYDFLEDTKKLSLDVLSEHRIRIRYINILD
                     SSIQSEEIYIRKKGARISLWARIKKAWNNKD"
     gene            complement(20086..21732)
                     /locus_tag="PLF66_06100"
     CDS             complement(20086..21732)
                     /locus_tag="PLF66_06100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63649.1"
                     /translation="MSDTSKRVLERSQTGEYQFTPYGEFLSYYHAHLQVFNKFIQEKP
                     VTQRDRDQIYQRVKGYMVSNVKKTDHFFEKIGDFASLLQMDVEILKRHLNDNFVDILM
                     KVQDKLREQEMEKLTSKKDFAHITEELVFKLGEIFPPNCRFAFHDGLLVIENISTGEV
                     VKPIGLLGAIKEEQQKAQEKLAARVTNKPKEPEYVPEKSILLEICEKHEDTLTGELLE
                     LKEEFPMNTPEPEPEPEPVKEKGLLDDVEELSFEMDESPAYNPEDTLPREPGLLDDIE
                     GIDFQQETQRTITIPEEEPQGNDDILDFLDSVGPSQSSARRQAVQVSTPEEEEEGVQQ
                     HEEEEDSTQNEYMDSNQSYSDEPEENSILDFEPEPEIEPEPELPDPADLFTFKDYSEL
                     TKKIQSYKSTNDTNGYNQWLASASDLEKCFVSIRANFTKEQTGQPVDWNKYFDTVASK
                     TQLKRSTIEKFKARVKHLDLTKAVLDIAIKELKNQSQEVISIMKSAWPHILTTFGEAP
                     DYDSVESKLKTLLSRLKSDSQRLPIQNIMLKGIQKLRQKL"
     gene            21899..22543
                     /locus_tag="PLF66_06105"
     CDS             21899..22543
                     /locus_tag="PLF66_06105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63650.1"
                     /translation="MSQMVNFVQEKKIVNLDYIHPENIYSHDAFFFKEIEPNESWDFQ
                     SQPDISLTSLGFLFIRSLLFQELQKTKLPVSNLSQSMKIQLAKDGYANYFNQFFETNP
                     IPSILTEQKTPELISDLFLENKSFHMKDKEKNADLLLGGGILKENKTNAHALIEKLFS
                     YFDLSNVYGHLFLRTKSSSYLYLLNPENKSKTFYIQQKNLFSIDFQFYHIKISK"
     gene            22540..23547
                     /gene="rfaE1"
                     /locus_tag="PLF66_06110"
     CDS             22540..23547
                     /gene="rfaE1"
                     /locus_tag="PLF66_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015680896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase"
                     /protein_id="HMZ63651.1"
                     /translation="MKIAKSSFYKTVENLNEIKLLVVGDAVLDEYLFGEVTRISPEAP
                     VPVVLVKEDKITLGGAGNVIKNLSAIGVKSQFYAKCGKDLKADKLKSLLHEEGLGIHD
                     LHLTESDQIPTILKTRVIASHQQICRIDREKVIPLSLTEEKKILASYTKSLEECNGVI
                     LSDYDKGYFNEKLITQLIQLAKKKKKFIAVDPQVRHFFLYKGISIMTPNHHEAGGALG
                     RKLITDEEVAKATIEIAKKLSTESMMITRGEKGMTLYSSKTKKVYHIPTVAKEVYDVT
                     GAGDTVISLYSAFRTAGMSDLESTLVANAGAGVVVEKLGASTVNLDELEKALIDRKLL
                     E"
     gene            23544..24023
                     /gene="rfaE2"
                     /locus_tag="PLF66_06115"
     CDS             23544..24023
                     /gene="rfaE2"
                     /locus_tag="PLF66_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012388629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HMZ63652.1"
                     /translation="MILENIKAKIIPLEKIQSFKSSIQDKPIVFTNGCFDILHIGHLT
                     YLSQARELGKFLWVGINSDLSVKGLKGPNRPINSEEDRSILLASLFFVDAVTIFPEST
                     PISILEKIQPDIHVKGGDYIAEQMPEYKTVINFGGVIKILPFVAGKSSTNIINKIKG"
     gene            24027..24644
                     /locus_tag="PLF66_06120"
     CDS             24027..24644
                     /locus_tag="PLF66_06120"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HMZ63653.1"
                     /translation="MLILKLFWQRILVICLLFICNTFIYSEDGICYQWSEGDALHLNS
                     CSVSTLESCSKGKKSIQKKDTEFQIYKGKLFKDNENNIYEFRKESKEECDKELIKSAR
                     EAELAMCSNWGKAVYASNLKIGDTAYIYGKSVNLRENPNTKAKKIAAPEDRTEVKILE
                     RSSKEDSIQNLYSAYWFKISVKGTIGWVYGQFLHPNPNSKEEFIQ"
     gene            24808..25038
                     /locus_tag="PLF66_06125"
     CDS             24808..25038
                     /locus_tag="PLF66_06125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63654.1"
                     /translation="MIKFLLLGLFIYLLYRMVRGFFQGIVIVTQIKRDQNSGEGFAFR
                     SPRTAEKDISSKARVLDEDKKIEEKSDITDVS"
     gene            25052..25543
                     /locus_tag="PLF66_06130"
     CDS             25052..25543
                     /locus_tag="PLF66_06130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63655.1"
                     /translation="MIRFLLLILVVFIANCGPSKGEFGWATTQDENKNILEREIYTVT
                     DFKMTRVNLIFSPSDTIHYVYTFSRHPGEGSKFIVTLEKESMGFVEIELKEKTIEPET
                     NSLKDKFIRLNPGHYRVQVVFEEEVIDNIEFDVLPEEGYTVESVDLDNDKEEQDDIIR
                     YSR"
     gene            25794..26963
                     /gene="sucC"
                     /locus_tag="PLF66_06135"
     CDS             25794..26963
                     /gene="sucC"
                     /locus_tag="PLF66_06135"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015678275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HMZ63656.1"
                     /translation="MKIHEYQAKEVLRRHKVNVPFGVVIDDKSQGQKAYDEVAKHSPI
                     VVVKAQIHAGGRGKGGGVKVTKTKEDALAAIDKILGMQLITHQTGPEGKKVLKVYLEQ
                     GIDIDKEFYLSILLDRSAKKTIFMASREGGMEIEEVAAKHPEKIIKVEIDTGVGIQPN
                     QAREIAYSLGLSGDAVKSAVPFFLSLYDAYMKEDCSLLEINPLILTKQNQIIAGDCKM
                     TFDDNAEFRHPDNLAFRDKSEEDPLELQASEFHLNYVKLDGNIGCMVNGAGLAMATMD
                     IIKLAGAEPANFLDVGGGANVTTVTNGFKIILGDPNVKAIFVNIFGGIVRCDRVALGI
                     VEAAKAVNIKVPLMVRLKGTNAEEGKKILADSGLTIIVADELSDAAEKIPKLLGK"
     gene            26980..27867
                     /gene="sucD"
                     /locus_tag="PLF66_06140"
     CDS             26980..27867
                     /gene="sucD"
                     /locus_tag="PLF66_06140"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002771466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HMZ63657.1"
                     /translation="MSVLLDKNTKIVVQGITGKEGSFHTSQMIEYNSHFGSKVVAGVT
                     PGKGGTKSEHGVPVFNTIKDAMKQEGANVSAIFVPPAFAADAILEGIFAEIPLIICIT
                     EGIPVHDMTRVYNVLKGSKSKLVGPNCPGVISPGKCKIGIMPGFIHKEGRIGVVSKSG
                     TLTYEAVGQLTNVGLGQSTCIGIGGDPIIGMSHIEALSLLSEDPDTDGIIMIGEIGGT
                     AEEEAAAFVKQNIKKPVVGFIAGQTAPPGKRMGHAGAIISGGMGTAKSKMDAMRDAGI
                     HVCVSPADMGSTMKKVLGK"
     gene            27896..29497
                     /locus_tag="PLF66_06145"
     CDS             27896..29497
                     /locus_tag="PLF66_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002974113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HMZ63658.1"
                     /translation="MKYKSLLLITLLIVATNLNAEDTLSEESIKLAQENAKPVTGEEK
                     PKKVLRLTLKEAVQRSVEFNPTIRNSKYELVKYDSGFLKSESKYSWRLVGGADIVQGV
                     LPYNQANILSGTRTQTNRYNMGIEKVFTTGTYFKIDASTQRFDNNAFEDPLTSPSGFR
                     GLAIPPLYTGAITATLAQDILKNTFGMQDRNNQKILKNQSEITKEELSLRLSNTIVAA
                     LVDYWNYSVSDSSVATFEQLLKNTKNIRDLTKQKTGLGLAENFEINQWNALYTQTENQ
                     LERAKLERDENKRKLIRNLNLTSDSEIGSISDMETNLPANLDYEKDLEYAYQNRGDWK
                     SMKLRREIAELSMKNAKDNALPSLKVSGSYAYQAQTTVSPQENFVNGTNGIPSFRYHN
                     LNANAKLTYPIGDTGVKAELRDAAILQKQVAISEEDLKKEIADEIRARVDAVLIGHKI
                     LQNAIKTRKESESYYNGLLGSFRQGRFTAVAVKNALDTLVQNELQETQAKINFNINIL
                     RYELAKNSLLKKFDIDVDKIVPDSF"
     gene            29746..31140
                     /locus_tag="PLF66_06150"
     CDS             29746..31140
                     /locus_tag="PLF66_06150"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HMZ63659.1"
                     /translation="MDSSDLKSIKAARPTNIARITSAFIFIILYSFYDYSVVLCFALL
                     QFTLSIFWFWLIEKRFAIYKKNPSLWYLPASIDVFFATASVYITGVSYSPVITAYILI
                     TCMSSVDLIRARGLFTTISSSVSFIFILFLTKFNIIPYFNILGDPNIEISTFSMVLSA
                     LLVTMACFTANSVIYQIYYQLNEKNSELSTSLDKINLLKLQQDADYALTARLMEPFGG
                     NFTNSNNIHIEFYLKQKKSFVFKNQDLEIGGDILIAEELYFNGNKYILFANGDAMGKS
                     MQGACGALVLGVISKSIIANAQSEKKENSIIPNLWLANMVSEIHRIFESFDGSMLASL
                     FIGIMEEKTGDLFYINTEHPQAILYRDDIASFLPSSLEYRKLGTQGIQPPFLHVEYTK
                     LVDGDVLFIGSDGKDDLIILNNGVKQMNEDERLFSRIIESAKADLDLILKQIEMKGEL
                     SDDLSIMKLSFHNH"
     gene            31512..32189
                     /locus_tag="PLF66_06155"
     CDS             31512..32189
                     /locus_tag="PLF66_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004479694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b subunit"
                     /protein_id="HMZ63660.1"
                     /translation="MSSTKSYLNTSVGKKVIMAISGLVLYGFVIMHMLGNLQVFSGPE
                     KLNAYGHFLQSLGGLLWIARLVLLGAVGIHIYVAVKLTRENMAARPIPYYNQRTNVAS
                     YASRTMRYSGLIIFAFIVYHLLHFTLGVTDPAGFALKDSIGRHDIYGMVISGFSNPLV
                     SGSYIIAMALLCLHLSHGFFSLFQSLGINQPEYDAKLKLVSNLFALGIFVGNSSMPIA
                     VLLKIVA"
     gene            32203..34116
                     /locus_tag="PLF66_06160"
     CDS             32203..34116
                     /locus_tag="PLF66_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016753085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HMZ63661.1"
                     /translation="MALNSKIPSGPIEKKWDKHKFELKLVNPANKRKYDVIVVGSGLA
                     GGSAAATLAELGYNVSCFCYQDSPRRAHSIAAQGGINAAKNYQNDGDSVYRLFYDTIK
                     GGDFRAREANVYRLAEISRNIIDQCVAQGVPFAREYGGHLANRSFGGAQVSRTFYAKG
                     QTGQQLLLGAYSALSRQIGLGKVKMYSRTEMLDLVLVNGHAKGIIARDMVTGKISSYS
                     ADAVVLATGGYGNVFYLSTNAMGCNVTATYRAYKKGALFGNPCYTQIHPTCIPVSGDY
                     QSKLTLMSESLRNDGRIWVPKKKGDTRSPDQIPEEERDYYLERKYPSFGNLAPRDIAS
                     RSAKEACDNGLGVGPGGLGVYLDFSAAIKRLGEDVIKDRYGNLFDMYERITAENPYKV
                     PMRIYPAVHYTMGGLWVDYNLMSNIPGLHVIGEANFSDHGANRLGASALMQGLADGYF
                     VLPYTIGDYFANNPSKKISTEAPEFKQAEAEVNARINKFLSIKGKKTVDTFHRELGKI
                     MWDYCGMARNKAGLEKAIKLIPELREEFWNNVNVLGTNEELNISLEKAGRVADFLEFG
                     ELMCRDALNREESCGGHFREEYQTEDGEAKRNDDKYCYVSAWEFKGENQKPVEHKEEL
                     VYENIKLAQRSYK"
     gene            34127..34858
                     /locus_tag="PLF66_06165"
     CDS             34127..34858
                     /locus_tag="PLF66_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010574591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HMZ63662.1"
                     /translation="MNLKLKVWRQKNSADKGKFVDYEAKDISTHASFLEMMDIVNENL
                     EKKGEDPIAFDHDCREGICGMCSMMINGIAHGHKKGTTTCQLHMHNFKDGDSIVIEPW
                     RAKAFPVLKDLVVDRGSFDRVMQAGGFISVNTGGAPDANALPIPKVDADLAMDAAACI
                     GCGACVASCKNASAMLFVSAKVSQFALLPQGQVERKERVRKMLNAMDKEGFGNCTNQY
                     ECEAACPKEISVSNIARLNREFLMA"
     gene            35101..36426
                     /locus_tag="PLF66_06170"
     CDS             35101..36426
                     /locus_tag="PLF66_06170"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HMZ63663.1"
                     /translation="MKVKTILLLLLSLFLFIISFLLRDPVLIKFRYSGLILINLIGVI
                     WLIAYLKHPEKKHLISKFSILLIVLSLGISSGKQFSFQFKKNFVENYNKEVLEKYASH
                     IIVGFRSEKELNDLLNLPVLGFFITHHNVKKLDLIKSKELIDSIQTNRKKLNFPKALI
                     ASDQEGGNVSRLSPPLELQPSLGDMISDNALLNEELSKKINDYADLQASELNKIGVNL
                     NFSPILDLKYKKEKNPLDLYSRIYKRAIASDPKIVSFVAEIYSKRLLENKILPTLKHF
                     PGIGRINEDTHFFNADLKTSLNDLEANDLYPFLHIAHNIEYPVIMLSHSTITELDKES
                     PISISEKGIQEYIRTKFPISTVLITDDMNMGPIMYRVGGIGNASVDALNAGVDILLIS
                     YDGEQIYEALYALCKADESKKLNQARILESDKRIKRLLFFSFPEIESFK"
     gene            36479..37285
                     /locus_tag="PLF66_06175"
     CDS             36479..37285
                     /locus_tag="PLF66_06175"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HMZ63664.1"
                     /translation="MKIVEDKLKGKWVLITGASSGFGIDFANIFAESGANLILVARRI
                     EPMNELKQRLEGLYKIKVEVISMDLSKKESPINLYNTIQAKNIQVDALINNAGFGIYG
                     EFVNTSWEQEEMQLMVDVVNLMHLTKLFLKDMVKRNFGYILNISSIGGYQPSPLYASY
                     SAGKSYVLNFTEALNYELRNTNVKVSTLAPGVTRTGFQSTAAKDKPLTLYQKLSMMES
                     RPVAEIGVKGLMKGTPSVLPGIMNKLSIFSLRFTPRRIMAFMVYHLMRID"
     gene            37286..38410
                     /locus_tag="PLF66_06180"
     CDS             37286..38410
                     /locus_tag="PLF66_06180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63665.1"
                     /translation="MISTVNFFYNQVSKNRIIFSFILLSFFLLGCKALSTKKKDSVNQ
                     TVHLVLNSTGYATEFCIFEVQKQENQSYECKAEKPLEYSRCVEYFADNTSYANLTPRE
                     TLDQIYKGLAELELARPSLKNRIKSVGYISSGSFNTAKPNESKQVLKEVEDYFISEKL
                     NVKSKLLTGDEEAKLTLKEILSKENISSAALLQIGTDTIEVITESNRESKVPSAKVGY
                     QTFLEEISTIKPGINACRQPISSFFKSESSSFDRCKQFLLENMSKPNKISSIGKTKLN
                     QYEEVFATGALFNYYYPNKNDLTLDELNSIGREYCSLTIDEIQKKGIKKKNAYNLCYA
                     VSYTSTLIEYFEIKKLKILPKEIYLKKLATSQTLFPEFCK"
     gene            38411..39322
                     /gene="rsmH"
                     /locus_tag="PLF66_06185"
     CDS             38411..39322
                     /gene="rsmH"
                     /locus_tag="PLF66_06185"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010568872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HMZ63666.1"
                     /translation="MTDFQLTHVSVMPEEIISYFTELGRENEIRFIDGTAGEGGHTQI
                     LLEHFPNSKVLLNDRDSIMLERASERLKKYLDRVFLREGNFSEILSKDIEEAFQTQKV
                     NGILLDLGISTFHLIGRERGFSFKTNENLDMRLDESGRLTAFDVVNKYKAEKLSQIFR
                     EYGEENWTKKIVEKIVLVRRKKPIQTTQELAKIVESVIPRKFWPDKTHPSFRIFQAIR
                     IEVNDELGHIKKGLEALLNLLDKDGIFCVISFHSLEDRIVKQVFREKSKDGKFEILTK
                     KPILPKEEEIKQNPSSRSAKLRVIKAL"
     gene            39319..39660
                     /locus_tag="PLF66_06190"
     CDS             39319..39660
                     /locus_tag="PLF66_06190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63667.1"
                     /translation="MIVTKFDKKEIKTTISIIALAVVKISLPASVLFLLVWQSIDHAH
                     LSRQIKKLSLKKEELYKKNYELKVGIATYTSAERMESIYKRNNQAANYVNKKISTLIL
                     PPENKTFEFYK"
     gene            39699..41213
                     /locus_tag="PLF66_06195"
     CDS             39699..41213
                     /locus_tag="PLF66_06195"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HMZ63668.1"
                     /translation="MLISELLKNFKKISLTSGNLSEPINYIQSDSRKLEENDIFCVYD
                     DFSDKTIDYLKDAKSKNIKTILIRKTSPYLDETEQFPNVLICSEDPMQLHGYIASFLK
                     DDPSKKCKIIAVTGTNGKTSMTYILYDIFSRENKNCGIIGTIETKYGNKVIQTGYTTP
                     DASTLNSVLADMVKEEIEYVFMEASSHGLKLGRLNGLHIYGALFTNLTRDHLDFHKTM
                     NDYLKSKFLLFKLLSKSCHENTFAAMSVDSSGSDKMLSLIKKAKLKINLLLLGKGKQF
                     ETKLNELSLKGIDFDFKEKGNQQVNFTTNLLGNFNFLNVSLAIVASRALGLETESIRN
                     TVGNLIPVNGRFQIVYNSKKTRVAIVDYAHTPDALQNVLKSMQEIPHSKIICLFGCGG
                     DRDKTKRPIMGSIAAKLADLVIITSDNPRSEDPELILDEIQAGFPKKFKSFLRITNRR
                     QAIKKGIEILPDDGLLLVAGKGHETVQIIGTKREDFSDARELIEAFSQDEISRR"
     gene            41221..42330
                     /gene="mraY"
                     /locus_tag="PLF66_06200"
     CDS             41221..42330
                     /gene="mraY"
                     /locus_tag="PLF66_06200"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011670912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HMZ63669.1"
                     /translation="MFQLIYDLFGSDIGVLRIFNYVTFRALMAGLTAMVLSFVLGKRI
                     IAFLYKLKFSETVRTDGPQSHAVKAGTPTMGGLMIISTLILSILLWGNLKNLNLILLT
                     VFSFLFSMLGFRDDYEKAILKIKGGMKSRVKFLLSVLIAFAFCFTFYFLTGEAHTAGR
                     GVSFTLTDLFLPFIKGPVINLGILAIPFSILVIIGSSHGVNLTDGLDGLATGTVMIAT
                     ITFGIIAYASGTPIAANYLNIPYLQGAHEYSVFLSALAGALLGFLWFNTHPAEVFMGD
                     TGSLFLGATLGMIAVMLKKEILLFILGGIFVAEAISVMLQVASFKLTGKRIFKMAPLH
                     HHFELMGIKETKIVIRFWIVGVILALFALSTLRIQ"
     gene            42332..43483
                     /locus_tag="PLF66_06205"
     CDS             42332..43483
                     /locus_tag="PLF66_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010679572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative peptidoglycan glycosyltransferase FtsW"
                     /protein_id="HMZ63670.1"
                     /translation="MIRFIREWFKNFTVIGKTPIDLPLIYIIIILLLVGISALFSGST
                     VTAHKLTEDPYFFLKKQAIWMGLSFFFFIVFAIIPYRIFQHYAVYLIVLSIVLLILVF
                     IPGLSKTVKTQAGRNFHRWIGFGPFQLQPSEFCKIAVLIYLSAFFVKLSHIQEKTIKT
                     YLIPGIAIGLILLLIMLEPALGTTLQISGMILALIFLNGFPVKKLVIGFLSTIPLILF
                     LVYRVSYWRKRIEVWLNPYSDKDGDGFQLYSSFRAFMESGWFGNQISTSHSHKSLPYN
                     HTDFIVATFVQDYGFIGFLVLLGLFTFLIVRGFSLVRRVNEPFGFLLGSGILLMISIQ
                     IIINLFVVTGLFPITGISLPFMSYGGSSLLTIMISLGILINITKREHIT"
     gene            43483..44559
                     /locus_tag="PLF66_06210"
     CDS             43483..44559
                     /locus_tag="PLF66_06210"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016547626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:0051991 -
                     UDP-N-acetyl-D-glucosamine:N-acetylmuramoyl-L-alanyl-D-
                     glutamyl-meso-2,
                     6-diaminopimelyl-D-alanyl-D-alanine-diphosphoundecaprenol
                     4-beta-N-acetylglucosaminlytransferase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HMZ63671.1"
                     /translation="MRSIIIAAGGTGGHISPGIALAESLMDQKQELGFESLAIHSLIR
                     NKDNPDLRDSPCPVIWHNTPQLSNFLTFPFLFLINFVKTVFQFRKLNVDCVIAMGGYS
                     SLPALLYAVLFKKKIFLCEQNRIVGKVTRVFHKYADKIAFSFPPVNENGFKNISYKIL
                     GNPLRKKIIPDTKSTSNKSQVVNKKEKLNVLVMGGSQGARQINNIVINSMNNPEIAKN
                     FSFRILTGTSLYDEAKAKTEKSADIISYSENMKTHYEWANLVIARSGAGVISECAVYG
                     LPQILIPYPYAADNHQVANAEYFENQAGAWVLNQTDENNTKLVSILVNIAKNRESLLP
                     ITEKALSAGRVNASSDTIQFFFKE"
     gene            44562..45938
                     /gene="murC"
                     /locus_tag="PLF66_06215"
     CDS             44562..45938
                     /gene="murC"
                     /locus_tag="PLF66_06215"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008593585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HMZ63672.1"
                     /translation="MRDKKPFFIGIGGSGMSSLAHILLDLKIDVSGYDKAQSDTVKKL
                     EMRGARITHDPENVNLDGIDYIVYSSAVKENHPVFQKALSLHKTLIHRSELLHELFSK
                     KFSIAVAGSHGKTTTTAMTGQILLEAGERPSIMLGGEVGYLNDRGGKWDNGKWGVYES
                     DESDGTFLNHKADIKIVTNIDNDHLDFYKTTDALLEAFAKFIHNKKSSTSIVYLGDKG
                     VEDAIDLLEDQKNIIGYIEASKAANLSAKFPELQIEPFEIFDNELSFFRQGEILKIKL
                     PYAGDHYLKNSFAALLAAETAGIEVHASIEILNGYTGVKRRLELLGNQNNIHVYDDYG
                     HHPTEILAVIQSIKRMRPVGAKCCILFQPHRYTRTRDHYEEFAKALDESDLLFLLPIY
                     SAGESPIEGISTELIYDHLVNKERTILLTGDISRDVSILKKYILSGDYLVSLGAGNVR
                     SWAEEFLK"
     gene            45946..46515
                     /locus_tag="PLF66_06220"
     CDS             45946..46515
                     /locus_tag="PLF66_06220"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016548801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HMZ63673.1"
                     /translation="MNEEELHNVGPSLIGQDWHHTNCFGCGPDNSRGLHADFPFDAQA
                     GEVRFKFVFEKFYEGAPGFVHGGILASLLDEAQGVMCFHAGHFVMTDQLFVKYDKACP
                     LEEEVECRAWITIAKNRRLYTKATIHSPTTGEIYAHSKARWYNMKEKVFMKMFHRSPL
                     RMERLKHILDLNKKRGKEIRKKIKSQKHS"
     gene            complement(46662..48410)
                     /locus_tag="PLF66_06225"
     CDS             complement(46662..48410)
                     /locus_tag="PLF66_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HMZ63674.1"
                     /translation="MIKSNYFTTNSDILLHFEKFIPWKQIVPIYENNFHDAKVFQQTG
                     NELLASAPTSVEEAVEYYRTILETAGDIAGNFVSEHVKSMDIQGAKYENGKVIHPKEM
                     IDSVNKAKESGIQAYGFQRKYGGLGLPFTVRALMGEIIYRVDTSFAIAFGCVNLGEIL
                     ERIASEEMKKDWLPRMAAGEFCAAMGLTEPNHGSDLPNIKTKATKQEDGKWVLNGTKR
                     FITQACGLGETPSIILTLARSGGNGARGLSFFLVHSKDIQVAGIEKKLGLHASPTCEV
                     VFENTPALLIGEEGFGLVRYTMGMLNGARMGIAAQGVGMATAAYEEAKIFASTRIQFG
                     KPIEEIPAIKKMLRRMEREIMAMRCLTLEGGRTVDMYYWRMEHLKEAGKSDKEISNDE
                     EVRHWMKLADLLTPVSKYYCSEMCNRIAYDALQIHGGSGFTEDYDIARIYRDARIITI
                     YDGTTQIQVMAAIGGVISGMAPTGHFRKYLLSEMEEFSPSSRLKTLFEKLEEAVSVYK
                     NISSAEVRDSFSLELVEVATRLLAGIQLEKTAKLLESESQTSRLKQADEFNIDSLAIA
                     LGNIEKMKNFANIEIL"
     gene            49063..50118
                     /gene="lpxD"
                     /locus_tag="PLF66_06230"
     CDS             49063..50118
                     /gene="lpxD"
                     /locus_tag="PLF66_06230"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002979015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HMZ63675.1"
                     /translation="MKEFNLKQIQDVIPNSKILNTDNPEAIIFNSLAPLNSDKEKFIS
                     FVSAKGHLSEAKKTKAVALIVNQEIASNFTDIPILIVDNVELQNAKILNLLFPPKTPN
                     GVISTTAIIHPSAKVGSNTQLGHYVVIGENSEIGQNCIIEAGVKIGDNVKIGNNVRIG
                     MNCVFHDGTQIGDNFIVFGNSTFGGDGFKFVASKGIHHKIPQVGKVIIGNDVEIGCNC
                     TVDRGGLTDTIIGNGCKFDNMVHIGHNVVIGDHVIIAGQSGVAGSTTIGNGVIIGGGC
                     CISDHLILPEGTIVAGGTGMRTSPKKPDVYIGWDWGMTFAEFQKARVNIKHLVTFNKW
                     ASRIKELEKKLGIKVEE"
     gene            complement(50126..50578)
                     /locus_tag="PLF66_06235"
     CDS             complement(50126..50578)
                     /locus_tag="PLF66_06235"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HMZ63676.1"
                     /translation="MEDLEKDLKEQIFGKSGGMRDDFGIKLPPNSALELQSKIIEYVP
                     KKSIAVQFPVQEKHTGPLGILQGGILTAFFDDTFGPLSFAVLKKPMVSIDIHVYFLRS
                     VKVGDTVTIRAEVTSRGRKVIFMKAEAYDQKNKLIATATSSALIFKEE"
     gene            51041..52438
                     /locus_tag="PLF66_06240"
     CDS             51041..52438
                     /locus_tag="PLF66_06240"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HMZ63677.1"
                     /translation="MKYIALIFLFLVYSCSKTNLEKINQYLEKGDYERLLEFANKNKS
                     NFKEEELYLSSVAVYQLQNSLRKNKSDENMVNSNYFQKLEKKIGLKIKTVTTDNSVLP
                     VIDDVYEKLIPKNSDYRIKILLDRFKYTYKTNDPTENTFLLLDILEQDPRQFLQEYKF
                     IWIEAMKLQLPKLLPDESKTKFNHFLHYLASKEDTDIKNHFFVTVGTNVNLRSGPGTE
                     NANIGKLNQEEVFQIDSDFNTVNIGNKTGKWVQIYIWKSDLAGWIFSPFLKNISWNQK
                     IADEFERDFIQKNNFSMIDFNNWNPEEIPNGFYGNYIPRNKEIIEANIGFPVYSSKTE
                     EAICKKLKKIPNKLLIHFYNKDTKEELTLFYLKSILDSGEKRKTLVKIIDRKLVLNQR
                     STEIELEKNTEQFIEIKIDNEEENTKLSLISMNNREYFDLLARKGEIIKSCEICIPQS
                     KKNSNVAFLFSFKIY"
     gene            52510..53448
                     /gene="ispH"
                     /locus_tag="PLF66_06245"
     CDS             52510..53448
                     /gene="ispH"
                     /locus_tag="PLF66_06245"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004460626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HMZ63678.1"
                     /translation="MKKIYLANPRGFCAGVKYAISFVENVHNLYQNEQIYVRKEIVHN
                     RRVVNDMEKKGIKFINDLDEAPENSIVIFSAHGVSPTVVQTAKDKNMRIGDATCPLVT
                     RVHRKAKKLKETHQIIYIGHEGHDEAIGTMGEAEMFLVESEEDVEKLLPRIDPNRPLT
                     YLMQTTLSVSDTKKVIDKIAEVFPNVEHPDKDDICYATTERQEAVDSMMDSVDAMLVI
                     GAPNSSNSMRLVQLAQKKKPKSFRVSSADEINKEYLDSLEIETMGITAGASSPQVLID
                     EIINKIKEFYPEIKVENFPTSREDSMNFKLPKELLK"
     gene            complement(53450..53908)
                     /locus_tag="PLF66_06250"
     CDS             complement(53450..53908)
                     /locus_tag="PLF66_06250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63679.1"
                     /translation="MKFKFAILSFIYINLIGSLAAETILLRNGQIIQGKVLGHDSESV
                     TINTEGATKVIPKSTVHKVIFSSNSAELQKVLQEKKKQAKLQKKSESNKPDDEEELAS
                     LDEMEDDKQDITKKVTILEQKIDKYEKRISNLRNKISKLKQKIKTKSTVE"
     gene            complement(54070..55206)
                     /locus_tag="PLF66_06255"
     CDS             complement(54070..55206)
                     /locus_tag="PLF66_06255"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: protein motif:HMM:NF004679.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HMZ63680.1"
                     /translation="MKRTEKLFLPSESKLGVMGSGQLGRMFAQKAKERGYSVSCYSPE
                     KNSPAFKAGCIEYVGDYTDKVSIREFLDNIDALTFEFENIPEAALSVIEEYVQNKNLR
                     VCPSVNSIRISQNRFKEKENFRKIGLNTTDYIYLNSLESLYENKNKIEYPCILKTNQF
                     GYDGKGQHKIKDFSDLEKIIKQSKSIDYIIEKIVDFSCEISIVAGRFDSGKILYYLPS
                     ENSHKNHILDISIHPASINHELTEAAIQAVKNLLSSLNYVGVLALEFFVKETQLICNE
                     FAPRPHNSGHFSMDASNFSQFEMQLHTLTNLEPDMEEIKTNPCVMKNIIGFDFMDLKE
                     TSIERLKKSEYKLHLYQKEEPKKGRKMGHWNYLGKLEAKDAFPE"
BASE COUNT        18413 a         9162 c         9098 g        18650 t
ORIGIN      
        1 gatatgacag caccagcaat atataaatat ttatagtatt ttctgaaaga agccaataaa
       61 caaagaattg ctgtataaga ataaatcatg atccaaatat agggatctgg atcattgtat
      121 tgaactaatg caaacaacgt aaataaaata aagagaataa agttgataat cctcacttta
      181 ataagcacct cttccttttt ggcttgcata gtagataact tttctagctc tcattacatc
      241 tcctaacggt ctatgaactt ttaatgcatt ccaaggatca aaaacagatt tttctatttt
      301 ttcattcagg gctttccctt cttccgagtc tggattttgt tttggaatcg ttagtttacc
      361 gagtgtaata taaggtgatt cggactccag ccaatcaaca gatgcatctt ctatcggagt
      421 aattttttca tttacaaaaa actgcatttg taaataaaac tctacatctt ccgttgctaa
      481 tctctttttt atatcaatac ttaaatctga attatttgat gaagtaattt tttgatttga
      541 aactggaagc aatcttactc ttcctgcata atctccacaa gcgataggcg ctgctgaata
      601 aaatgtttca gtcataaatc cagaaaaagg cttcccaaag gtttttgctg ttttaacaac
      661 tctttgaaaa ccaccaatga caccatatgt tccaaatagg tgtttaatta gtgaaaaagg
      721 actctcagaa gcagcaatta cgagtgctat aaaaggagct gattttggaa atgcaaaagc
      781 agattggtta attagggtaa aatcctgact gtctgtattt cctgatccca atgcgttagg
      841 accattctga cctaaaacct ttaaagcaaa ccctcttata tccggaactg aatcaggttt
      901 aacatctact ccaccatttg aaattcgaat caaagcatcg aaagtttgcg ctttcgcgaa
      961 taaacctact tttgcataat caggtatgtt tcctaaaatt tctagttttg cttttagtcc
     1021 acatataatt tttctatgca atgctctacc tgtgccatat tgtgccgatt ttttcttttg
     1081 aatctcctcg atagatttag catatccatt gaatcttact tcttcatcag aagctatttc
     1141 ttctttccat tctttgcttg gtgcacccat ttttagttcc ttctcaattg aattattttc
     1201 aaagacagtg atcttttcta atttgttttt aagatatgct tggtaaacta atttttaaaa
     1261 aatatgatcc cttgcggcta ataaaagtaa atgaatgaaa ttcgaagaat gaattcaatg
     1321 attcgaattt tctgtatgct ataaaaagta gaaaatcccc tttattcatg ggatgttctc
     1381 ttctcataaa taattaaaaa gctattgtga acaataaaaa ccttacttga tttaaaagaa
     1441 tctgaaaact tagatcagtg atttcgaaag ggaactaaaa agtgcaggaa ttggtagctc
     1501 ctgtctttaa attgcaggat acagatttta ctcgtttaac actgcgtata tcactcacga
     1561 gatctttttg agattgaggg aatccttcat tcaaacaggt ttgggcatct gcttttactt
     1621 gacttgcagt agtggtagaa ggaagagttg ctgctaaaag caaatctgga gtattcacaa
     1681 ttttatccgc tgcgattgca attctacctc tggcagaatt taaaaaaagc aattccaatt
     1741 ctgaaatcag atttgctgca tttgaaaaac ctagagattc gcaactgtaa accgttgaat
     1801 atgtattatt agcgattaat gaatctgtat ctgtttgatt tgtaattgta tcggaggata
     1861 tacctgaatt taaaaaagaa aagttacacg aaggaactgc ttttaataaa tcatttgcat
     1921 cacttagatt gtatgttttt tctccatttg ttagaattag atcatttgga ttacaaagac
     1981 ctttagaacg tcgatcgcaa tacgcaatgt tatttgtatt gtcttttctc aaacaattcc
     2041 ctaaactcct ttgttgtatt agataaagag aaaaaatatt atttcgattc ctagtgtctt
     2101 ttttattttg aattccatct aaattcgaac aagagttaat aaataaaaat aagaagatta
     2161 ttaaaattat aatttgattc attagaatct tacctctatt ccaaaattaa ttaaaggtaa
     2221 ataaatgagt ctccctccag gcaatggaga ttgcacataa ggggagttta atgtatcata
     2281 aacaggagat gggtttacac catttaaata aggtttcgta ttttcaaaat tctgaccgga
     2341 aatatttctt ctgccaagta agtttataat ttcaatatac ccatttacca atccccattc
     2401 ataattcata aatcgatcca atcgtatatc gacctgttga aaagacgcta accttgcaga
     2461 attataatca tttgaataga gtggattatt tagattgatt ccaaagcttg cagcttgagc
     2521 aattctagta gatcctacga taggcgttat tggagtgttt gtataatatc ggaagcgacc
     2581 cccaagttgc cactcacttg aaattttcca gccaaatact atattcaaaa gatgagttct
     2641 gtctaaatca taaagctctc gcctatcgtt atcaaaaagt agttctaatt ggttatcatc
     2701 ataataatta acataattat caccaacttc tgtttgataa agtaacttac gggtgttatt
     2761 tcttaagttt cgattcgtaa cttccgaact agcaagtctg gtttgatgat tatttctttt
     2821 ggtgattgaa ttagaatagg atatccaacc aaaaaaacca ttttcccctg gtttatttga
     2881 tttctttaaa taaatctcta ccccttgaga aaatccatct cctctgtttg agtaattgag
     2941 cgctttatct gttaaaatgt ttctagaaac aaaatcgggt ttttgtatta tatctctcgg
     3001 ttcattatta aatgcgtatg ggtttctcgc aaaaccatcc gatacaacta agtcagaaaa
     3061 aatatttcta aagccttcaa ctttaaatag ccattgttgt ccgatttctt gatttactcc
     3121 taatgccata tgttctgctt tttccataag caactttgag tttcctactt tacttgaaac
     3181 ttgttcaatt ccaacaggag aattcctatg aattcccgca ccagcaatga aggtagttcc
     3241 agttttttta atcgtatagg aagtattaac tctgggactt aacgcttttt gatttgccaa
     3301 attatagtaa tcccagcgta agcctggaac taatttgaat cctgcataat caagcgttgc
     3361 ctccccatac gcgccatatt cttttgcttt cgctccatct ccatcaatca aagctctaaa
     3421 tgtgggcgag ctatctaaaa gatcattgaa cactttggta aattgattat tttgctgagt
     3481 gatattttca ccttttagag taatgtttct ttccctataa ttttgtcctg tttcaaattt
     3541 taaaatattt ttaatcagtt ctagagattg tatgttctca acataatata aggtttgagt
     3601 tgtaacgttt tgtattccaa aaatattctc ggcggttgca ggatttgtaa tttttacttc
     3661 aaaaaactct ttaaaagtat ttctggaaat actcaatgta ttagaaaact tagatgttgg
     3721 ttgataaatg tatctaaatc cctctgtata aaaactctta tctaaaccga taggcggtct
     3781 attgtctgaa cctcctcctc cacttcctgc actttcaaat tcagcttgtg cattggtata
     3841 cgcctgtcta tccctaacac caaaaagaga aaatgtaatt ttatgcttat ctgaaaaatc
     3901 atgtccatat ttaaattgat aatcttgata atcggcatac tttgcatttg gcgggattgc
     3961 atcaggataa aatctaagaa gtaaaagatt tggataattt tttctagccg cagcaatcat
     4021 ataagatttt ttagaaagtt tagtttcaat taatgcgtca gataaaaaga aattcatatt
     4081 gatgatagca gaagttttct ttacatccga cttcccttct atattaataa ttccacctgt
     4141 tgcataacca taacgagttg agtaagctcc agtatatata tcaaaggatt tgattatatt
     4201 attattaaaa acagaagatt ggtttcctag atgaaaagga taactaacag gaaatccatc
     4261 aaaataaaat tgatttgccc ttgctccagc acctcttaac actacatctc ctctatcact
     4321 attactatag gggcttccat tgatagttgg aattaaggct gctgttgtag aaagaccaat
     4381 tggaattgca ggcgatacac ctggcaaagt taaaattgct tttagggaat ctccttgagc
     4441 accaggcaat ctttttattt cttcttgttg taatgtatat ctagagagct tcgttttttc
     4501 tttttcgccc gctacttcga ttgcaccttt ttctttttct cctacaaaaa aaaataattc
     4561 ttgtccatca taataaatct cttttctttt tacctcaaca tcagtaccag atataattcg
     4621 aagcgtataa tatccattag ccggaacttg aaaatcaacc tctccattgg catttgtgat
     4681 aaaatatcct ccggattctt ttattacaat agttgcattc gctttagggg aattagtgga
     4741 tttatcaata attttaattt tagcagagat aggtttttct tgtgaaaaaa tactatttgt
     4801 aaaaaccaat agaattaaaa acagcctgag cactttcaaa atattctcac acaactccat
     4861 ggatatgttt tgaatgggca tctagcattc actaaatcta gttcgcactg atttacttct
     4921 ccaggaggac gatcattaat aaacaagagt ggttcttttg gacgattccc acattggctt
     4981 gcttttgatt cgattaacaa agcaactcgt ttttgtattt cccaagaatc aattctattt
     5041 ttatcatagc agtttagaca aaataaaatg aatatactga atctaattaa ttttgccatc
     5101 tattctataa attgtaaact gatccttcat tccaggaatt tcatattgaa tctcttccat
     5161 tctaattttg ttagactcta gaaattttat cacatcggga ttttctttat attgctttgt
     5221 aaaaactatt tgggatgcac ccgcaagttt ttgaatctta gctgcaatat ttactgtctt
     5281 gccaaagtag tcaatcccgc tatttaaatt tactgcaata cactgtccgt agtgaattga
     5341 tatcctgagt cgaattccat ctaaattctt gccatcaaat ttatgaatga gcaaattggc
     5401 tgcttgaatc ccatttccag gcgaaggaaa agatgccatc actgcatctc caatagtctt
     5461 gataatcgca ccctgatttt cttgaactat cttatgaatt tcttcgaaat gtctttttac
     5521 ttgtacgaag gtttttgaat caccctgaat atcatataaa ctggaagaac caactatatc
     5581 agtaaaaaaa actgtctgta gacccaattc tagttttaat tcactagaaa ttgcttctgc
     5641 cgaaaaaaga tcatggaatt cctgtaatgt aaatatataa gcaggcttta aaacaaaagg
     5701 atcccaagca agttcctcta ttgtatatag atttattttt tctttaggat tttcaatccg
     5761 tatcaaagga tttgttcctg agagaaaatt ggtgttatcc gctgaaaacc aattgattaa
     5821 ctttttttct tctgtatctg ttatatttag gatattaaaa tttctttccc ctttgattct
     5881 gagtcgatag gatccaacat ttaaatttaa attttcttcg ataaactgtt ttggacctaa
     5941 aactttctgc atctttatat gcgtcttctt cgcaggctct gcactacaaa agaacacttt
     6001 tggcacattc cgtattgaag gatgaatatg aaacgttatt tctatagagt tttcagaatt
     6061 attatcgaaa tcaatctcgc agacttcgca gctacctttc gctggaactt caaataatgt
     6121 taccgcttct tgtctagttc ctctgcaatg cggacaaata atatcccagc taatagtaag
     6181 taatcctaat ctagttgcat gcatacaaat aattaagagt tctctttcag aaaccttcca
     6241 tttattagca agttctaata ctcgaattcg atataaatcc tgatcatcac ctgtttcaat
     6301 aaattccaat aatttcttta ttatatctat atttaaattt ttttctaata attgtttctt
     6361 taccctttca aataacgttt tattttctat tttataataa gtttcagaag atgttaaaag
     6421 agatagagct tttttatttt tagatagctt cacaatttca ttcaaagcag ttttatattt
     6481 attaaaaata attttctttg aagcagataa tagtaattta tagaaaaatc cgcgcggtat
     6541 ccagccaaga tatacatata gttttgttgt tttatcattt attttttcta aatggaaaat
     6601 ggcttttacg gtataagcaa aacctttgga atattctcta atactaacta gatattttcc
     6661 actcacccag ttccatggaa cttcaatcca ttctaaatca attccagcat tttttgcctt
     6721 tccgtataag attccttctt tctcttcaaa agtcatttta ctcaaaccca tcgcttggtt
     6781 tagtctagaa gtgtcgctta taaatggcca taaagagttt ggttcaattt ctagttcaaa
     6841 attccaaaac caatccattc tatttggttt gtttgctgta tatttttctt cccacggata
     6901 aagctttaaa aattcttcta atgttaaact catagatgca tatgtttatc aaaatataat
     6961 agattacaag tcgtaaaatt tatttaaaat agaaaaagaa ttgttgttaa tttgtttata
     7021 tatatacata tgtgtctaag aagtcagatc atagaaatga agcttgagaa gtctataacc
     7081 aataacagag caattgtaga aatagaaggg tatcttgaca tgggaacgtt tcgagatttt
     7141 gaaatgtttg tgcaagcaac ctctaatgaa gagaaagtag ccaatattgg actaaatcta
     7201 tctaaatgta gagcaatcga ctcttcgggt atgggttgta ttcttcggat tatgaacaat
     7261 ctaaaaggta aaaataaaaa gctttttctg ctagatgtgc catcgaatat aaaaggcata
     7321 ttaaaattaa gtcaagttga caaatacctt aaaattctat cttctgaaga attttccaaa
     7381 gaaacttaat ctattttcag tatagcccaa gcctgactca tacttggtaa aaaactatac
     7441 aaatcttaaa ttctaaattt taggtttaag attttattta aaaataaaaa aatagctaat
     7501 cactgttatc cccaaaataa ttctataata cccaaaactt gaaaaactat ttttttgtat
     7561 aaaagctaaa aaccaacgga tgataaacat acaaaatatg aatgtaagaa aaaatccaag
     7621 taggagtagt attaatttat ctccatgtag aagcgatcta tacttataaa gcttgtaaag
     7681 acttgctgct attaaaacag gtatcgctag aaaaaaggaa tactctgctg aggttttttt
     7741 agggagacct aaaacccgag cagtaataat ggtcattgca gatctagaca ttccaggtat
     7801 gagtgccaag cattgaaaaa ttccgattgt gatcgactgc ctaaaggtta aaccaattat
     7861 ctcattttcc ttttcattgt tatttttgct taaaagtctt tctacaatta caattgcgat
     7921 tccacctgtt aaccaagcaa aacccaatat cagtaacaat acttcacttg cttttatttt
     7981 atctaaaaaa ctcttaaata aaaatccaat aattaaaaca ggaaaagaac caattgcaac
     8041 atttataaaa aataccaatc cagaagaatc ttttattgca cctgacgcat attgaatacc
     8101 ttgctttgct ttatctagta tgtatctaaa atataaaact aacacagata agatagcacc
     8161 gctttgaatg aatatatcaa ataaatcatc aaagctagct ccatcctcta atccctcaaa
     8221 aggaaaaatg gatgtaaata gaaataaatg tcctgtagat gaaattggta aaaactctgt
     8281 gatagattct ataatacttc taaggattaa atttaaataa ttattcatag ctggatctct
     8341 tttttttgaa tgaagatatt tgcgaatttt aaattgagtt tgttctaatt aaagaaaacg
     8401 tcactcttat acaaaagttt tagagtgacg ttgtttggat taaaaattat aaatgagaat
     8461 tatttatttt ctgaatttgc tggtttttct tctgtctttg aatcaggagt tgacttagat
     8521 tctgttttgt ctgaatcctt ttctttaaga tcttttttca ggaaactagg tcctgtcttt
     8581 aggtattgtt ctaaatcaaa tttttcattg tgtttgattg agataccttc tttgactcgt
     8641 tcaatcactt taggtaaaat cttttctgat tcttgttgtt ttaaaacacc tcttccaatt
     8701 tgcacacaac gatctagtgt aacgttttgc atacgtttgt ctttttctct taattcttta
     8761 cacttattgt ttgcgtcttc gtcagttatc ttaatttttt tctcaacttg ctctgttata
     8821 taaagctgtg atatggtttg catttctaaa tatttgacaa tatttttaat atcaggtcta
     8881 gttgaaaaac cagctttatc agcagctgtt tttatcatta ccatattacg atagttttca
     8941 taaaaatttt taggttggaa ctgatagtta attcctttga gttgctcatc tagctcttct
     9001 atatcgctgg aaattatttt aataacattt tccttttcca cattttgcat acgactcatc
     9061 gactcgattg cagtctcata tgtggtctca aactttttaa ctgttaattt ttctccgtca
     9121 agtgtttcaa taacgctact attatcatca tcgcaaccac caaaagcaaa tagagataag
     9181 attacgagat ttattaaaat tagttttgat ttcattcaat tctttccttg ttaaaaacaa
     9241 attattttca gaaattggca cgtctagcgt ttttttgtca ggaaatcttt tgcaataatt
     9301 ggaaaatctc tgttagtctc tgtttttctt ttttattctt gggtttatat cttagaacac
     9361 ttggttcttt gggttgaatc gataagccct cacgtgaaga aatgagcttc atgattttat
     9421 ttgtatcacc tttgaaatgc tgaccagatt tcaattttat ttcttcattg tcttcagtaa
     9481 taaattcgaa gcctaaacca gatcctaata ccctgatttt ttctaattgt aagaatgtct
     9541 ccaccacttc tggaaatttt ccaaaacgat cttccatatc ctttgctatt tcatccatct
     9601 catctagatg aagtgcaccc tcaaatcgtt tgtaaaattc aattttttgt tttgtatctg
     9661 gaatataatc ttctggaata tagaagttag acgctaagtt aactacagtt cttacctcta
     9721 cagggatttc ctcttttttc aacttgctta ctgcttcatt tagcatttga acatataaat
     9781 caaatccaat ttccataatg gatccacttt gttccgttcc tagtagattc ccagcacctc
     9841 taatttctaa atcgcgcata gcaactttaa atcctgcacc aagttcttgg tattcagaaa
     9901 ttgtatttaa tcttttttct gcttgttcag tgagtgctct attttctgga taaaatagat
     9961 aagcataggc ttttcgacca cttcttccaa ctctaccgcg aatctgataa agttgagaca
    10021 aaccaaaagt atccgctctt ctcacaatca ttgtgtttac attgggcata tcaattccag
    10081 attcaatgat tgtagttgtt accagtagat catattttcg attgttaaaa tctaaaatgg
    10141 tttcttctac ttcttcctca tttaattgtc catgcaatac tccaatggaa atattgggaa
    10201 ggatctttcc taactctttt gcttcgttct caattgtttc cactcggttg tgtaaataga
    10261 agacctgtcc acctctttcc aattcttttt caattgccaa tttcattatt tcttcattgt
    10321 cttctaatac aaaagtttca acactttgcc tatttttagg aggagtttca atgattgaca
    10381 aatctcgaat accagtaagc gacatatgaa gtgttcttgg tataggtgtt gcgcttaacg
    10441 tcaaaacatc tactagattt ttaatttttt taatcgcttc tttatgattt actccaaatc
    10501 tttgttcttc gtctataata agtaatccta gattcttagg tttaacattt ccgcttaaaa
    10561 gtgcatgcgt tccaattagt aaatccaatt ctcccctaga aaattgagca atagcatttt
    10621 taatttcaga actagaccta aatctagaaa ccattccaat ttttacaggg taattttcaa
    10681 aacgatttgt gaatgtatta aaatgttgca gagctaagat ggttgttgga gaaatgaata
    10741 gaacttgttt tcctgccatg acagctttga atgctgcgcg tatcgctact tctgtttttc
    10801 cataaccaac gtctccacaa atcaatcgat ccataggacg aggcgattcc atatctgctt
    10861 taactgcctc aatcgcactg agttgatctg gagtttcttc atattcaaat tcagcttcaa
    10921 attcttcctg ccaaatcgta tctcttggga agctatatcc ctgtaacttc atacgattgg
    10981 aatacattac tagtaattct tctgctaaaa catctatgat tccttgcgct cgttcttttt
    11041 tctttttcca agtgcttttt ccaaggctat ctaagactgg tttttcggaa ccacccacat
    11101 acctttgcac caaggagatt tgatcgagag gaacaaaaag cgtatcaccg cctgcatact
    11161 caagctttaa aaaatctcta atcttagaat cagcagtaac tttttcaatt ttaataaact
    11221 tgccaattcc atgattgata tggactacat aatcgccttc cttaagatcg ataaagcttt
    11281 gtatggcgcg tgacgatgtt tttttgaatc tagattttct cttgtaactt cttccaaaaa
    11341 tatcattttc tgtccatata tgaatatttt cttcaggaag aataaaccca tttttaagtt
    11401 cagataaaac taaataagct cccgattttt taggaaaggg aatgatttct ggttcttccg
    11461 aactttcatt taataattga atattttctt tttcaaagag accttttaat ctttctgttt
    11521 gtgcatgaaa agaagatgtg atgacaagta ttccatcttt cattgtttca attttttctc
    11581 ttacatctct aatctttcct ttgaaggaat ctgtttctag gattggaaga attgcttctg
    11641 tatcttttaa ttcactcggt ttgataagat gaaaactaat ttcttttttt gcgattagta
    11701 atgagtattc tttctctaag gaaattattt tttccggttc taggcaaata atatccgttt
    11761 ttcttttttc atataaggac ctgtattctc tttcaatttg aaaagtttta tccaccactt
    11821 cattttgtct gtgatagatg atgagaggtt tggttttaaa ataatcaaga attccatgtg
    11881 gagtatggac gacaggcaca agctcttcta atgcgttatg atgagccatc tccatatcag
    11941 gtttttttag attttttcct ctgacttcaa tttctctttg gtagagttcc cattcctcct
    12001 ttgataataa aaattcgtct acaggaagaa tggatacatc ctctaattca gataatgatc
    12061 tttgcgttgt tgaatcaaaa gttttaatcg attctaatgt atctccaaag aaatccaatc
    12121 ttactggatc tgaagacaaa ggagagtaaa catcaatcac tcctccttta acagaaaact
    12181 ttccatattc atcacaaatt tcttctcggc tataacctaa agcaacaagg gaggaaatta
    12241 attttgtttg atcaaagtca tcccctttct ttaggttgat tgatttaccc ataagtattt
    12301 tgggagaagg aagagttttt aagaaaccaa tagtggatgt aaataagagt aatcgttctt
    12361 cgtttaagat tctagctaaa gtattgattc tatcaatttt aatttctcta gaatatttgg
    12421 catagtcata tggaatcact tccggaccag gaagataggc tatactttta ctcggtaaga
    12481 aactaagagc ttctctatac agtgcttctg agtctgagtt ggttgcggag attactactg
    12541 ccgatttttt attttgagaa agatatagaa cggacgctaa aaaggaactt gctgaatctg
    12601 gtattccatg aaacctgaat tgcccttttt tcgaatagat tgtttcaaaa tttgtttctg
    12661 aaattttttt tacatagagt gaaagaatgt ctttcatatc caactaccat aattttaaag
    12721 tggattaatc ttaaaatgag attttaaatt taaatagata gaaagacttt accgattttt
    12781 tacttgtgag gtagcaaatg tttatttggg taactcaggg aattgtagaa ccctatattc
    12841 caaaggtcag accagatact gttaaaaaaa tcaacccaac acatagagga attccagata
    12901 aatctataga agtgggtgat ccagaggata ctattgtaat ctctcacaac aaaacaggtt
    12961 ttgcatcaga cgcatatatt gatgttgcag atatttattc ccaagaacag tccatcctta
    13021 aagaagaaag aaaacaaatc ttttatgcaa aagatatcat gacaaaaaaa gtaaaatgca
    13081 ttgcaccgga tgatttagta acaaaggtcg aggaaatttt tcaggaagat agatttaaac
    13141 atatgccagt agttgattca aatcaattta tacaaggaat tttatctgaa agagatttgc
    13201 taaaatttac aatagaattg ctacgcaaga ccattgaaaa caatcaagca aaagtgcaag
    13261 acattatgca aagaaaggtt ctatctgctt atcctgaaac tccgattcga gaaattgcaa
    13321 gaatcatgtt tgaagaaaaa gtgggatctg ttcctatcct agataaagaa aaaaaacatt
    13381 tagtaggaat tgtaactaga aatgatatac tcagaaaagt aatgaacaat ccgccacttg
    13441 agttatatac ataaaagaga ggataattcc aattaacctc ctttaatcga tcaacgaatc
    13501 tcctgctctt gttggaagta tatcatgatt gtcattgaac tcaggaaaga aatcaaaaaa
    13561 taattttctt gggacaactc tgtatctttc tggattttta ttcataaatg tgcctgtgaa
    13621 gctaacaagc ggtaaactat gaaaattaat tctatcttca aatccataaa aatcttgttt
    13681 cgtaaaacga tgcgtttgtg tgcgaatcac ataatctttt acaaatgacc aatctaactt
    13741 gaagtgcata cgggttcctt ggaaagcagg agatctttcg atcaagttta caagtcgatt
    13801 taatggacaa agctccttgt agtattgagt aacaaaaacc ataacaaatc tttgtgacaa
    13861 tcccattggt ttccaaatta aatcagaaag attctccatg ttttgttcat atttaatgtc
    13921 ttctaaaatt ttatgtaatt cttgatgtcc tttttctttt tttaaatagc tgatatgttc
    13981 ttcgtatcca aattcgccat aataaagcca tttatataaa tcttgtaatt caaaataagg
    14041 atgctcttct ataaattgcc tgagagcaaa aacccgctga tcgttattta caatagaaag
    14101 tgtatcaatt gacattggtt ttatttttaa atttgagttc tgttccgtca ataattcttt
    14161 ttatattttc atggtgagta aataaaacta aaaagaaatt aaaaacgact agtataaata
    14221 attttgaaga ataattttct ccttcataga gcctatagaa aataggtaga aagaaaaaac
    14281 cagcgataga gccaagggat gcaatttttt tccaatagac aacgatgagt ccaagcacag
    14341 cactcataag acaaggaatc ggtaatagat aaaggtaaat cccgattgaa gtagcaatcc
    14401 cctttcctcc ttgaaaacca agaaaaggac taaaaatatg acccaaaatg gagaacaccc
    14461 ctcctaaaag aagaatccat tccttttcta aaaaggcaga ttcgatagaa aacaaaggga
    14521 agagaaaaaa aggtaaaatt ccttttccaa catctataat taaaacgata aagcctgact
    14581 tccatcctac agatcttaat acattcgttg cacccggatt cccacttccc attttcttaa
    14641 tatcgattcc ttgcattttt gaaacgatat gcgcacttgg gaaggaaccc aataaaaaac
    14701 taatgaataa aaatatgaat tccattattc ccgataatcc ttttcttttt tccgatcaga
    14761 tcggatttct aattccactt gcaccccatt taattgaaat tcttctgtaa ttcgattttt
    14821 gataaaactt aaaagtggtt ttttaaataa atcaacatga tttacaaata aaagaagttt
    14881 gaaaggtgtg gtggaaactt gagttgcgta taaaattcga ggaagctttt tagattgaag
    14941 tcccatcttt ccttcagaca tccattctga tagtttttga tttattttgg aagtagaaat
    15001 cttagtattt acttttttat gaatcttaac acattcttct agaactttgc gaatcctttg
    15061 cttcttttgc gcgctaatac taatcaccgg taaagcggct gtcggatgaa atcttgaaat
    15121 taaatccttt ttatattcac taaaagtatt tgaatcttta tcttctatta gatcccattt
    15181 gttaatcgca agaactaccg gctttccact ctttcgaatg attgcaaaaa ttttcttatc
    15241 gtattcccct aaccctttag atgcatcaat aatatgcact acaatatcag agtcctcaat
    15301 tgattgtaga gttcttttgt aggaataaaa ctccaattct tctgaacttt tactgttctt
    15361 tcgaattcct gcagtatcaa cgatcagtat gttcatatct tcaaatttta aaacactatg
    15421 aactgtatct cttgtcgttc ccggaatctc actcacgaca gctctttcgt atcctaagat
    15481 tgcatttaaa aagctagact ttcctgcatt tggtttacct actatacaaa tactaaaatc
    15541 tgtattttgc ttttcgccca ttctcacttt tgcaagatag aagttaatct tatcaaatag
    15601 aatttttagg tttcttcgac caagtgctga gattggaatt acttcctgta atcccaatcg
    15661 ataaaaatat tccaaatcaa aatcattgtc tggattgtct gatttattta cacaacagat
    15721 gatatttttt ttaacaaatc ttttgtcggt taaaaataat tttatgagtt ttgaatcata
    15781 atctaaaatc tcatttctat caattacata tacaatgatg tctgattcaa gtaaatgttc
    15841 aaagctgatt tggatgatag aggaagttag atcatttata ttttctatat caagtcctgg
    15901 tgtatctgat aaagtaaatg gtttaatgaa attttcattt tcaatatcat gacttaatac
    15961 atcgcgtgta acccctggaa agtcatgggt aatagcaact ttttgtttta aaaaacaatt
    16021 aaataaggta gactttccca cattctgtct accaactatc gatactactg gtaatttttt
    16081 caagatctgt tcctattttt taatgctcta tctatttcga tttttgcttc tttcttttgc
    16141 acagattctc ttttatcata gagctttttc ggtttggcaa tcgcaagctg taattttaca
    16201 aattgctttt cattgaaata gatttttact ccaactagtg ataaaccctt ttctttgatt
    16261 tgtttttcaa gacgaataat ttcttttctg tgtgctagta attttcttgg gcggacttct
    16321 ggatgatttg catatccacc atttttataa ggagtaatat gaaaattttg aagataaagt
    16381 tctccgtttt ttacttgtgc aaatgcgtca gttaaattgg ctctcttttc tcttaagctt
    16441 tttacttcag atccagataa tacgattcca acttctaaaa attctaaaag ctcaaaatca
    16501 aatctagcct ttttattgac tacaatttcc ttgtagccag tttctttttc tttttccttc
    16561 tgtttcataa ttatcttact gcatgatgaa catgaatgag tcttgttaat tcggttccaa
    16621 ttaaatttgg catatttgaa aaattatccg gtgttatctg caccgaattt tcgaagagat
    16681 tttcagcaat gtatctatta ccaagaccaa cggctagaaa tacataattc ttcttaacat
    16741 tcttcaaaac ttcttctttg agctttttgt tttcccaaga ttctatttca cgatctattt
    16801 ctgtttttcc tctttgtcct ctgaagtcgg agataattac gattatcctc gtttcagcct
    16861 ctggtgtaaa atatctctca caatcctcaa tcagagaaaa ttcataaact gaatctccct
    16921 gccaatcttt cgtgagagaa ttaaacatct cctcttcttt tcttgtatcg taatcttctt
    16981 ctttatcttt tagacatatt aaatcaatat gatctttacg attgagtcta tccgaataag
    17041 tatagattga aaattttaca ttgtgctcat ttaaaatata agcacttgta agcatagccg
    17101 aaagagtcgc taccgaatat tcaaaattga aaattcttct agctttactt actaaaaata
    17161 cgatttcaac gccttttaac ttttcctcat ttttatcaat gatagtctta tcaaatactt
    17221 ttgaatctcc tcttccactt ttaaaagaaa tgtatttgcg cgtatcgact ctttttccct
    17281 gtgtttgata cattctggta atgtctattt ctggatcaaa tagagactct aaattcatct
    17341 ctacttcctc taagcttttt tcaaatacct ctttaaactc tttcagacta acggcgatag
    17401 aatagttcca tagaattttt cttttagcga gaaactgttt ataaagctct tctgtgcgat
    17461 aaccccatgc accaccgcca ccagcaggct caccttgacc catatttcct tgggtttctt
    17521 ttccatacca agcatccgca ccttcttttg tattgccccc tgtctccgga gtattgatat
    17581 caattccttt ttttaaagga ctacctgaat tttttaatgg cgctttaccg gtattagcct
    17641 ctcttttatg aagatttgga tcccaccatg gatttctatc tagatttacc ggttccgtaa
    17701 aagttttttt tttctcctct atcttttgat aaaggtcttc aggaaatttt tttgctttta
    17761 cggcagtatc tataattctt tccatatcaa acctctcctt tggatcggcg atttgattta
    17821 tataggaaat cttagcacct ctcaccacaa cttctgaaag attttctgga tgattgtatt
    17881 ttaaaatatg gtctttccat tttagtaaat ttgacaaacc aaataaatac ggatttaaat
    17941 ttccagatcc aataactctt ttttcagaaa gagttttaat ctttctatga aaagcactca
    18001 tgatgtcagc taacgtttca tctacaatat aaaactgtaa aatctctttt aaggtttttt
    18061 catcctccaa atttgggaac aggattggag taaaacggtt tctaaatgct cttgaaatag
    18121 taggaatcga tttagttttt ctaaacattt taaatgcaaa gactctcgaa tcttccttta
    18181 aatgaagtgg tctttcttct ccactctcag gaggaagagt gattgccttc gcatcgtcag
    18241 tgagcatgtt taatttttca actaactcag ctccagcagc ttcgagggct gtaatgacta
    18301 tcgttccccc ttctctaatt cccctggtta atggaccatc gatccattct acctttgaac
    18361 cagacaccgg ttttaatgca cctaatatgt ctgatgtatg aattccttta cataaattaa
    18421 catgggaaag tttaagacct gaaatttgtg taatgagaga cattaaaaaa tttggatcat
    18481 cttcctctcc aaactcaatc aatatgtttt cattgagttg gattgcagtg attaccttct
    18541 caataaattc caaagttttc tcagtaattg gaatttcaga taagatcttc ttagaaatag
    18601 tttcatctgt atgaatactt ttatcgttac aatacagttt gtctttttct acgtagagtt
    18661 taatttcttg ttttgtttct tttaccgaat actgcaattc ttgtgaaact aattcttctt
    18721 gtgatttttt atcttccgct tttctaaatg gttctataaa taaattatat aactctctgt
    18781 atacgatgga cttatcgtta cagttaaaag ctagaagtct ttcacagatt ttttttaagg
    18841 tgcggatatt gaaatgatat ttttctaaat cgcctttacc aatttcattg tttaaaactt
    18901 ttttttccgt tgaaagagta attcgaatgc aactttgtaa aagtgattct gctagctcgg
    18961 ggtataattt ttttaagata aataaaattt cttctggtgg atatggatcg atgaaaacag
    19021 tagaaaaatt cttagttata tcaaatggaa gtggttttct tccctcaaaa ccttctgatg
    19081 gattttgggt tcctataaat ccaaaacctt cctttgcaga aatcctactt ccatctcctt
    19141 ccattaaatc aatgtaggca gattcataaa ctgtagcaaa gcgcttgatt acattcggag
    19201 agcaaagatt catttcatct gccacgaatg tgtacccatt tgatatggca tttacgatag
    19261 gaccatcaat ccattcaaag cctgcaccac tcattaggag tcgatacgat ccaatcaaat
    19321 cttctggaag tgtgtcctca ttaaaactat accgaagagt aggatgattt cgtttggaat
    19381 taatataata aatcagtgca ttttttccta cacctgcatc cccgactaaa agcacaggtt
    19441 gtccttctaa aataggaaaa agtatatttc tcaaattctt tagagttgta tctgtttcca
    19501 ctagattgga aggataaact aaggaaggat ttgtattttt gtaaacggga acatcaattc
    19561 cggcaatttt tgtagattcc ataaactgag tatttgcagg tataagttgg aagcaagtag
    19621 aaaaaaggac tgttatagct tcaaatccga tttgtaattt aaggattttt tccagaaatg
    19681 gatttaatga agcattctct tactaaaaca ttattccttc ctatttttct atttattctt
    19741 ttttgtgatt tagaaaacag atatccagat gcaggtgagt tctgcactcc aatgaaagaa
    19801 actccagatt gtgtaaaaat ggattttaga aaaaaagaat tagaattaca aaatccaaat
    19861 cgcattttaa aactaaacat gattactcgt gttgaatatg attttttaga agatacaaag
    19921 aaactttctt tagatgtttt aagtgaacac agaattcgaa tccgttatat aaatatttta
    19981 gattcctcaa ttcagtctga agaaatctat attcgaaaaa aaggtgctcg tatttctctc
    20041 tgggcaagaa taaaaaaagc ttggaataac aaagattagc cgcaattaaa gtttttgtct
    20101 taacttttga attcctttta gcattatgtt ttgaattggc aatctttggg aatctgattt
    20161 caatctagat aaaagcgttt ttaatttaga ttctactgaa tcataatctg gagcctctcc
    20221 aaacgttgtt aaaatatgcg gccacgcaga tttcataata gaaatcactt cttgggattg
    20281 attcttcaat tctttgattg caatatccaa aacagctttt gttaaatcta gatgtttaac
    20341 tctggcttta aatttctcaa ttgtagatcg ctttaattgt gttttagaag caactgtatc
    20401 aaagtattta ttccaatcca ctggctgccc agtttgttct tttgtgaagt tagcccgaat
    20461 tgaaacaaag catttttcta aatcacttgc acttgcaagc cattgattgt atccatttgt
    20521 atcatttgta gatttatagg attgaatttt ctttgtaagt tctgagtaat ctttaaaagt
    20581 aaatagatcg gctggatctg gaagctctgg ttctggctct atttcaggtt ctggttcaaa
    20641 atcaaggatc gaattctctt ctggctcatc tgaataagac tgattggaat ccatatattc
    20701 attttgagta gagtcttctt cctcttcatg ttgttgaacc ccttcctcct cttcctcagg
    20761 ggtagaaact tgaactgcct gccttctggc actcgattga gagggaccca ctgaatctag
    20821 gaaatctaga atatcatcat ttccctgtgg ttcctcttct ggaatggtaa ttgttctttg
    20881 tgtttcttgc tgaaaatcga taccttctat gtcatctaat aagcctggtt ctctaggaag
    20941 agtatcttct ggattgtaag ctggagattc atccatttca aaacttagtt cttctacatc
    21001 atcgagtaga cctttttctt taacaggctc cggctctggt tccggttcag gagtgttcat
    21061 tggaaattct tcttttaatt ctaataactc acccgttaat gtatcttcat gtttttcaca
    21121 gatttctagt aaaatagatt tttcaggaac atattcaggt tctttcggtt tgtttgttac
    21181 acgcgctgcc agtttttctt gtgctttttg ctgttcttct ttgatagcac ctagaagtcc
    21241 gatcggtttt actacttcac cggttgatat attttctatc actaaaagac cgtcatgaaa
    21301 tgcaaatcta caatttggag ggaatatttc tcctaattta aaaactaact cttctgttat
    21361 atgagcgaaa tcttttttag aggtaagctt ctccatttct tgctcacgaa gcttatcttg
    21421 cactttcatt agaatgtcta caaagttatc atttagatgt ctttttagaa tctccacatc
    21481 catttggagt aaactagcaa agtccccaat tttttcaaaa aagtggtctg tcttttttac
    21541 attggagacc atataacctt ttacacgttg atagatttga tctctatccc tttgcgtaac
    21601 tggtttttct tgaataaatt tattaaagac ttgtaagtgc gcgtgataat aggataggaa
    21661 ttctccatat ggagtaaatt ggtattcacc tgtttggctt ctttctaaaa ctcttttact
    21721 tgtatcagac atagcaattc ctatttagac tattatcgga atgaaaactt aaatgcagga
    21781 aaaaattcca atgcgtaaag gacagtatat ttaactacaa acattataaa agtcaaaaat
    21841 tatatccttt ggcgttgaag attcaatttt cctacttcta gtttccgaaa ataaatatat
    21901 gagtcagatg gtaaattttg ttcaagaaaa aaagatagta aatttagatt atatacaccc
    21961 agaaaatatc tattcccatg atgcattctt ctttaaagag atagagccaa atgaatcttg
    22021 ggattttcaa tcacaaccag atattagtct tacaagtctt gggttcttgt ttattcgatc
    22081 cttactcttc caagagctac aaaaaacaaa attaccggtt tctaacctaa gtcaatctat
    22141 gaaaattcaa ttggctaagg atggttacgc aaattatttt aatcaatttt tcgaaacaaa
    22201 tccaattcca tccatactaa cagaacaaaa aactccagag ttgatttctg atcttttttt
    22261 agaaaacaaa tcctttcata tgaaagataa agaaaaaaat gctgatttac ttttgggtgg
    22321 tggaatatta aaagaaaata aaaccaatgc gcatgcactc attgaaaaat tattttcgta
    22381 ttttgattta tccaatgtat atggacatct ttttctaaga actaaaagtt cttcgtattt
    22441 gtatttatta aaccctgaaa ataaatcgaa aaccttttac attcaacaaa agaacttatt
    22501 ttctattgac tttcaattct atcatatcaa gatttcgaaa tgaaaatagc gaagagtagt
    22561 ttttataaaa cagtagaaaa tttaaatgag atcaagctcc tagttgtagg cgatgcagtt
    22621 ctagatgaat atctatttgg agaagtaact cgaatttctc ccgaagcccc agttccagtc
    22681 gtacttgtaa aagaagataa aattacactg gggggagctg gaaatgtaat taaaaactta
    22741 agtgctatcg gggtaaaatc ccaattctat gcaaaatgcg ggaaagattt aaaagcagat
    22801 aagttaaaat cccttctcca tgaagaaggt ctcggcatac atgatttaca tctaactgaa
    22861 tctgaccaaa ttcctaccat cttgaaaaca cgtgtgattg cctctcatca acaaatttgt
    22921 agaattgatc gggaaaaagt aattccattg tcgcttacag aagaaaaaaa aattttagcg
    22981 agttatacaa agtctctaga agaatgcaat ggagtcatat tatctgatta cgacaaaggt
    23041 tattttaatg agaaattaat tacccaactc attcaattag ctaagaaaaa gaaaaaattc
    23101 atagctgtag atccacaggt tcgtcatttc tttttgtata aaggaatttc tatcatgaca
    23161 ccaaatcacc atgaagcagg tggtgcactt gggcgaaaac taattaccga tgaagaagtt
    23221 gcaaaagcaa ccattgagat tgcaaagaaa ctttcaactg aatccatgat gataactcgt
    23281 ggggaaaaag gaatgacctt atacagtagt aaaacaaaaa aagtatacca tattccaact
    23341 gtagcaaaag aagtttatga tgtgacaggt gcaggtgata cagtgataag tctctactct
    23401 gctttcagaa cagcaggaat gtctgacctt gaatctacgt tagttgccaa tgctggagct
    23461 ggagtggtgg ttgaaaaact aggagcatct accgttaact tagatgaatt ggaaaaagct
    23521 ttaattgata ggaagctatt agaatgatac tagaaaatat caaagctaaa attatacctc
    23581 tagaaaaaat tcaaagtttc aaatcatcga ttcaggataa acctatcgtt tttacgaatg
    23641 gttgttttga tattttacat attggtcatt tgacctattt gtctcaagca agagaactgg
    23701 gaaaatttct ttgggtagga attaactctg atttatcagt gaaaggatta aaaggaccca
    23761 atcgaccaat taattccgaa gaggacagat cgattcttct tgcttctctt ttttttgtag
    23821 atgcagtaac catatttcca gaatccacgc ctatctctat tttagaaaaa attcagccag
    23881 acattcacgt gaaaggtgga gattatatag cagagcaaat gcctgaatac aaaactgtta
    23941 taaattttgg gggcgttata aaaattcttc cttttgttgc cggaaaatct agcacaaata
    24001 taatcaataa aataaagggt taaagcatgc taattctaaa attattttgg cagaggatac
    24061 tagttatttg cctcctcttt atttgcaata ctttcattta ttctgaagat ggaatttgct
    24121 accaatggtc agagggagat gcactccatt taaattcttg ttctgtctcc actttggagt
    24181 cttgtagcaa agggaaaaaa tcaattcaga aaaaagatac cgaatttcaa atctataaag
    24241 gtaaactttt taaagacaat gaaaataata tttatgagtt tagaaaggaa tcaaaagaag
    24301 aatgcgataa ggaattaatc aaatctgcaa gagaagcgga acttgccatg tgtagcaatt
    24361 ggggcaaagc tgtgtatgcc tctaatttga agataggcga tactgcctac atttatggca
    24421 agtcagtgaa tctaagagaa aatccaaata caaaagcaaa aaaaattgca gcacctgaag
    24481 atagaacaga agtaaaaatt ttggaaagat ctagtaaaga agattcgatt caaaatcttt
    24541 attcagctta ttggtttaaa atctcagtta agggaactat cggctgggtg tatggtcagt
    24601 ttctacatcc aaatccaaac agcaaagaag aatttattca ataacacatc ctttgctgtt
    24661 ctaaaccaaa ggttttaaaa atctccctat gatcgtttca aaatcaaatc cgaaaaaaag
    24721 cataaaaaga agaaaatatc tcattcggta gaaatagagt atcttaaaaa acatttgacc
    24781 ccctagtaaa ataagggagt attaagtatg attaaatttt tactcttagg tctatttata
    24841 tatcttcttt atagaatggt tcgaggtttt tttcaaggca ttgtgattgt tacccaaatc
    24901 aaacgagacc agaattcagg ggaaggcttt gcatttcgct ccccgagaac agcagaaaag
    24961 gatatttctt ccaaagcacg cgttttggat gaggataaaa agatagagga aaagtcagat
    25021 ataaccgatg tttcttagca gggagatacg attgattcgt tttttacttt taatactggt
    25081 cgtttttatt gcaaactgtg gaccttccaa gggagaattt ggttgggcaa ctactcagga
    25141 tgagaataaa aatattctag aaagagaaat ctatactgtt accgatttta aaatgactcg
    25201 cgttaacttg atcttttctc cctcagatac aattcactat gtatatactt tttctagaca
    25261 tccaggagaa ggatcgaagt ttattgttac cttagaaaaa gaatcaatgg gttttgtaga
    25321 aattgaattg aaagagaaaa ccattgaacc tgaaacaaat tcattaaaag ataaattcat
    25381 ccgcctaaac cctggacatt atagggttca agttgtcttc gaggaagagg tcatcgacaa
    25441 tatagaattt gatgttctcc ctgaagaagg ttatacagta gagagtgtag acctagacaa
    25501 cgataaagaa gagcaagatg atatcattcg atactctcgt taatcgaagt atcctgaaag
    25561 tccacttccg attttcttct ttataaaaag caaagttctt tactaacaca attagaataa
    25621 agcacactta gaagggttta ctgctcttcc ctagatgaaa tatgaattct ttatctatgc
    25681 aacaccttat cttggcgtga ttactttttt ttgttggcaa taaagcgatt tataaaagag
    25741 taatatccaa ggcgtagttt ctgcgctctt ctaatttaaa tcagggatat agtatgaaaa
    25801 ttcacgaata ccaggcaaaa gaagtattac gaagacataa ggtaaatgtt ccatttggtg
    25861 ttgtaattga tgataaatca caaggtcaga aggcttatga tgaagttgca aagcattctc
    25921 ctattgttgt tgtgaaagca caaattcatg caggagggcg tggaaaaggt ggcggtgtaa
    25981 aagtcactaa aaccaaagaa gatgcattgg ctgcaattga taaaatttta ggaatgcaac
    26041 tcataaccca ccaaaccggt ccggaaggaa aaaaagttct aaaagtatat ttagaacaag
    26101 gtatagatat tgataaagag ttttatctta gcattttact tgatagatca gctaaaaaaa
    26161 ctatctttat ggcatctcgt gaaggtggaa tggagattga agaagttgcc gcaaagcacc
    26221 ctgaaaaaat tatcaaagta gaaattgaca ccggtgttgg aattcaacca aaccaagcaa
    26281 gagaaatcgc atatagctta ggattatcgg gtgacgcagt aaaatctgcc gttccatttt
    26341 tcctttctct gtacgatgct tatatgaaag aagattgctc tctcttagaa atcaaccctc
    26401 ttatcctcac aaaacaaaat caaatcattg caggcgactg taaaatgact tttgatgata
    26461 atgcagagtt ccgacatcct gataatctcg cgtttagaga taaatctgag gaagatccac
    26521 ttgaactcca agcatctgaa ttccatttga attatgtaaa attagatgga aacattggtt
    26581 gtatggtaaa tggagctggt cttgcgatgg caaccatgga cattataaaa ttggcaggag
    26641 ctgagccagc taacttctta gatgttggtg gtggtgcaaa tgttacaaca gtcacaaatg
    26701 gatttaaaat tattttagga gatccaaacg ttaaggctat ttttgtaaat atttttggtg
    26761 gaatcgttag atgcgataga gttgctctag gtatcgtaga agctgctaaa gctgtgaaca
    26821 taaaagttcc tctaatggtt cgactcaagg gaacaaatgc ggaagaagga aagaaaattc
    26881 tcgcagatag tggattgact atcattgtag cagatgaact ttctgatgct gcggaaaaaa
    26941 tccctaagct tttaggaaaa taatcgaaag gaagaatata tgtctgtatt attagataaa
    27001 aacacgaaaa ttgtagtgca aggaattacc ggaaaagaag gttctttcca tacatctcag
    27061 atgatcgaat acaatagtca ttttggttcg aaagtagtag ccggagtaac tcctggaaag
    27121 ggtggaacta aaagtgaaca tggagttcca gtatttaaca caatcaagga tgcaatgaaa
    27181 caagaaggtg caaatgtaag cgcaattttt gttcctccag cttttgctgc tgatgcaatc
    27241 ttggaaggta tctttgcaga aattcctttg atcatttgta ttacagaagg aattcctgtt
    27301 catgatatga ctcgagtgta taacgtccta aaaggttcta agtctaaatt ggttggacca
    27361 aattgtcctg gagtgattag tcctggtaaa tgtaaaatcg gaattatgcc tggcttcatt
    27421 cacaaagaag gaagaattgg agtagtttca aaatctggaa ccttaaccta tgaagcagtt
    27481 ggtcaattga ccaatgtagg tttaggacaa tctacttgta ttggaattgg aggagatcca
    27541 attattggta tgagtcatat tgaagctctt tctttgttaa gtgaagatcc tgacaccgat
    27601 ggaattatca tgattggtga aattggggga acagctgaag aagaggcagc agcctttgta
    27661 aaacaaaata ttaaaaaacc agtcgttggc tttattgcag gtcagactgc tccaccagga
    27721 aaaagaatgg gtcatgcagg cgccatcatc agtggtggaa tgggaactgc aaaatcaaag
    27781 atggatgcaa tgcgtgatgc aggaattcat gtttgtgtat cccctgctga catgggaagc
    27841 acaatgaaaa aggttttagg aaaataatta actaaactcc tgtaagagga atgatatgaa
    27901 atataaaagt ttgcttttaa taactttact gatagtagcg acaaatctaa atgcagaaga
    27961 tactctctct gaagaatcaa ttaaactagc acaagaaaat gccaaacctg ttacaggaga
    28021 agaaaaacct aaaaaagttt tacgcttaac actgaaagaa gcagttcagc gttcagtgga
    28081 attcaaccct accattcgca attcaaagta tgagttagta aaatatgatt ctggtttttt
    28141 aaaatcagaa tcaaaatatt cttggagact tgtgggtggt gcagatatag tgcagggagt
    28201 tttgccttac aaccaggcga atattttatc tggaacaaga acacaaacaa atcgttacaa
    28261 tatgggtatc gaaaaagttt ttacgacagg aacctatttt aaaatagatg cgagcacgca
    28321 gcgttttgat aataacgcat ttgaagatcc tttaactagc ccttctggat ttagaggact
    28381 tgctatccca cctctctata caggagcaat tacagctact cttgcacagg acatactaaa
    28441 aaatactttt ggtatgcaag atagaaataa ccagaaaata ctaaaaaatc aatctgaaat
    28501 tacaaaagaa gaattatccc ttcgcctttc taatacgata gttgccgccc tcgtagacta
    28561 ttggaattat tctgtttctg attcctcggt tgcaacattt gaacagctac ttaagaatac
    28621 taaaaatatt agagatctta caaagcaaaa gacaggttta ggtcttgcag aaaattttga
    28681 aattaatcag tggaatgcac tttatacaca aacagaaaac caattggaaa gagcaaaact
    28741 ggaaagagat gaaaacaaac gtaaattaat ccgaaatctt aatttaacaa gtgattcaga
    28801 gattggtagt atttccgata tggagactaa tttacctgca aatctagact atgaaaaaga
    28861 tttagaatat gcttatcaga atcgtggtga ttggaaaagc atgaaactca gaagagaaat
    28921 tgctgagtta tctatgaaaa atgcaaaaga taatgcgctg ccttctctaa aagtaagtgg
    28981 gtcttatgct taccaagcac aaactacggt ctcacctcaa gaaaattttg taaatggaac
    29041 aaatggaatt ccatctttcc gttaccataa tttaaatgcc aatgcgaaac ttacttatcc
    29101 aattggagat acaggtgtaa aagcagagct aagagatgca gccattcttc aaaaacaggt
    29161 agctatctca gaagaagatt taaaaaaaga aatcgctgat gaaataagag ctagagtgga
    29221 cgctgtctta attggtcata aaattctcca aaatgcaatt aaaaccagaa aagagtctga
    29281 aagctattac aatggactcc taggaagttt tcgccaaggt agatttactg cagtagcggt
    29341 aaaaaatgca ttggatacct tagtgcaaaa tgaattacaa gaaactcaag ccaaaatcaa
    29401 ttttaatatc aatatactac gttatgagtt agctaagaac tccctcctaa aaaaattcga
    29461 tattgatgta gataagattg tccctgactc tttttaataa tccgaataac aaatagtaaa
    29521 ctcacgtcca aagtaagcac tgagtttact ataggtttaa tttattctta aattattttt
    29581 tgaattcttt ttttgggatt ctttaaaaat ctttccaaga atagaatttt ctttacctaa
    29641 gtctttcctt tttaataagc tgcctaaaga cctaggagga aaattttaaa tatagacttt
    29701 tttgaggcta ataaggataa ccctcttatt gttggaaact tttttatgga ttcttctgat
    29761 ttaaaatcaa tcaaagcagc acgccctaca aatattgcta gaattacaag tgcgtttatt
    29821 tttattattc tttattcttt ttatgattat agtgtagttt tatgttttgc tcttttacaa
    29881 tttacactat ctattttttg gttttggcta attgagaaaa gatttgctat ttataaaaaa
    29941 aatccttctc tttggtattt gccagcatcg attgatgttt tttttgcaac agcatctgtt
    30001 tatattactg gtgtatccta ctcacctgtt attactgcct atatcttaat tacttgtatg
    30061 agctcagttg acttaattcg agctagaggt ttatttacaa ccatttctag tagtgtatct
    30121 tttattttca ttctattctt aactaaattt aatattattc cctattttaa tatattagga
    30181 gatcctaata tagaaatctc cacattctcc atggttctat ctgcattact tgtaactatg
    30241 gcttgtttta ctgcaaacag cgtaatttac cagatatatt atcagttaaa tgaaaagaat
    30301 agtgaattaa gcacatcttt agataaaatc aatttactca agcttcaaca ggacgcagat
    30361 tatgcgttaa ccgcaagatt aatggagcca ttcggaggaa attttacaaa tagtaacaac
    30421 attcacattg aattctatct aaaacagaaa aaatcttttg tgtttaagaa tcaagattta
    30481 gaaattggtg gagatatttt gattgcagaa gaattatatt ttaatggtaa taaatatatt
    30541 ttatttgcga atggagatgc tatgggtaaa tccatgcaag gtgcctgtgg tgcgttagtt
    30601 ctaggtgtta tctccaagtc tatcattgca aatgctcaat cagagaaaaa agaaaattca
    30661 atcattccga acctttggtt agcaaatatg gtttcggaga tccatcgaat ttttgaatcc
    30721 tttgatgggt ctatgttagc ctctctattt attggtatca tggaagaaaa gacgggagat
    30781 ttgttttata tcaatacaga acatccgcag gcaattctat acagagatga tatagcaagt
    30841 tttttaccct cctctctaga atatagaaag ttaggaaccc agggaattca gcctcctttt
    30901 ttacatgtag aatacacaaa attagtggat ggtgatgttt tatttattgg ttcagatgga
    30961 aaagatgatt taatcatatt aaacaatgga gtaaaacaaa tgaatgagga tgagaggttg
    31021 ttctctcgaa tcatagaatc tgcaaaagct gatttggatt taatactgaa acaaatagaa
    31081 atgaaaggag aattatcgga tgatctaagt attatgaaat tatcttttca taaccactaa
    31141 tctatgtctt aagcccaact atccaaataa atgtagaatg gaattaaaac atttttatgt
    31201 attgattcta cttattaatc tcgatccctt tcctaaaaat accaatttct aaaatactta
    31261 accattaaag attgatttta ttgattcatt aggtttctct aatattctca atattgctat
    31321 ttaagcaaaa tacattcaga tcgcttacga ttagcaattt aaacggaaaa aatgtaagaa
    31381 ttttaggaaa aaggatttta cacagaggga ttcgctttaa acagttaaat ttagactttt
    31441 gtttgaaaga tcaaccgata ttaaaacaaa aaattaattt ttaactagaa acttaaggga
    31501 gtatatagga aatgagctct actaagagtt atttaaacac ttcggtcggg aaaaaagtga
    31561 tcatggcaat ctctggttta gtcctgtatg ggttcgtgat tatgcacatg ctaggaaatt
    31621 tgcaagtttt ctctggacca gaaaaattaa acgcatatgg acattttttg caaagtttag
    31681 gcggtcttct ttggattgca agacttgtat tattaggggc agttggtatt cacatctatg
    31741 ttgctgtcaa actcacaaga gaaaatatgg cggctcgccc tattccctat tacaatcaaa
    31801 gaacaaatgt agcatcctat gcatctagaa ccatgcgtta ttcaggatta attatatttg
    31861 cttttatagt atatcaccta ctacacttta cgttaggtgt aacagaccca gctggctttg
    31921 ccttaaaaga cagtattgga agacatgata tttatggaat ggtaatttca ggcttttcta
    31981 atcctttagt ctccggttcc tatattatcg ctatggcact tctgtgttta catttaagcc
    32041 atggtttttt tagtttgttt cagtcgttag gaatcaatca acccgagtat gatgcaaaat
    32101 taaagttagt atcaaacctt tttgctctcg gaatatttgt cggtaattct tcaatgccga
    32161 ttgctgttct tttaaaaatt gtagcctaat aaggagtaac caatggcatt aaattcaaaa
    32221 attccttccg gtcctattga aaaaaaatgg gacaaacaca agtttgaatt aaaacttgta
    32281 aaccctgcaa ataaaagaaa atacgatgta atagtagtag gatctggatt agctggtgga
    32341 tcagctgcag ctactttggc agagcttgga tacaacgtta gttgcttttg ttatcaagat
    32401 agtccacgtc gtgctcatag tattgcagca cagggtggta tcaatgctgc taaaaattac
    32461 caaaatgatg gtgatagtgt ttatcgatta ttttatgata ctatcaaagg tggagatttt
    32521 agagcgagag aagcaaacgt gtatcgttta gcagagatta gccgcaatat cattgaccag
    32581 tgtgttgctc aaggtgttcc ttttgctcgt gaatacggtg gacatcttgc gaaccgatcc
    32641 tttggaggag ctcaagtttc tagaaccttt tatgctaaag gtcaaacagg acaacaatta
    32701 cttcttggag cgtattcagc tctatcaaga caaataggtc ttggtaaagt aaaaatgtat
    32761 agcagaacag aaatgctaga tctcgtttta gttaatgggc atgcaaaggg tattatcgct
    32821 cgagatatgg tgactggaaa aatttcttcc tactctgctg atgctgttgt tttggcaact
    32881 ggtggatacg gaaatgtttt ctatttatca actaacgcaa tgggatgtaa cgtgactgct
    32941 acttatcgtg cttacaaaaa aggagcgctc tttggcaatc cttgttatac tcaaattcac
    33001 ccaacctgta ttccagtttc tggtgactat caatctaaac ttactcttat gtcagagtct
    33061 cttagaaatg atggaagaat ttgggttcca aagaaaaagg gagacacaag atctccagat
    33121 caaattcctg aagaagaaag agattattat ttagaaagaa aatatcctag ttttggaaac
    33181 ctagctccta gagatattgc atcaagaagt gcaaaagaag cttgtgataa tggtcttgga
    33241 gttggtcctg gtggactcgg tgtttatcta gatttctctg ctgcaattaa aagacttgga
    33301 gaagacgtta ttaaagatag atatggaaac ctctttgata tgtatgagcg tataacagca
    33361 gaaaacccat acaaagttcc tatgcgtatc tatcctgctg ttcattatac aatgggtgga
    33421 ctttgggttg attataattt aatgagcaat attccaggac tccatgtaat tggcgaggcg
    33481 aacttttctg atcatggtgc aaacagacta ggtgcatctg ctttaatgca aggtcttgct
    33541 gatggttatt ttgtattacc atatacaatt ggtgattatt ttgcaaataa tccgtctaag
    33601 aagatttcta ctgaggctcc tgagttcaaa caagcagaag ccgaagtaaa tgctagaatt
    33661 aataaattcc tttctatcaa aggcaaaaaa acggtagata cattccacag agaactcgga
    33721 aaaattatgt gggattattg tggaatggct cgaaacaagg ctggactaga aaaagcaatc
    33781 aaactcattc ctgaattgag agaagaattc tggaacaatg tcaacgtatt aggaacaaat
    33841 gaagaactta acatttcctt agaaaaggct ggtagagttg cagatttctt agagttcggt
    33901 gagcttatgt gtagagatgc actcaatcga gaagaatctt gcggagggca ttttagagaa
    33961 gaataccaaa ccgaagatgg agaagcaaag cgaaacgatg ataagtattg ttatgtttcc
    34021 gcttgggaat ttaaaggtga aaatcaaaaa ccagtcgagc acaaagaaga attggtttat
    34081 gaaaatataa aactcgctca aaggagttac aaataatatg agtactatca atttaaaatt
    34141 aaaagtttgg cgtcaaaaaa actctgctga taaaggtaag tttgtagact atgaagcaaa
    34201 ggatataagc actcacgcat cttttttaga gatgatggac attgtgaatg agaaccttga
    34261 aaaaaaggga gaagatccta tcgcttttga tcatgattgc agggaaggaa tatgtggtat
    34321 gtgttcgatg atgatcaatg gaattgcaca tggacacaag aaaggaacta ctacttgcca
    34381 attacatatg cacaatttta aagatggtga ttccattgta attgaaccat ggcgagcaaa
    34441 agcatttcca gttttaaaag atttagttgt agatagagga tcttttgatc gagttatgca
    34501 agctggcggt tttatatcag taaacactgg tggggcgcct gacgcaaatg ctcttccgat
    34561 tcctaaggtc gatgctgact tagcaatgga tgcggctgct tgtattggtt gtggtgcttg
    34621 tgttgcttct tgtaaaaatg cttctgcgat gttgtttgta tctgctaaag tatctcagtt
    34681 cgctcttctc ccgcaaggtc aagtagagag gaaagagaga gttagaaaaa tgctaaatgc
    34741 gatggataag gaaggttttg gaaactgcac aaaccaatat gaatgcgaag cagcttgtcc
    34801 aaaagaaatc agcgttagta atatagctcg actcaatcgt gagtttttaa tggcttaggt
    34861 taagtctaaa aaggttcttt aaaaagatac cattactagc ttgggaggta aagatgcttt
    34921 gttttcttta cctccttttt atataaatca acaaaataaa ggataaatca gagtaagtca
    34981 tttctagtcc tttcgtttta ttttcttaaa aatttacaag agctaaatat actattgatt
    35041 gaatatcgat ttgatttttt tataatctta aatcttcttt actaaacact tctaaaaaaa
    35101 atgaaagtta aaacaatttt acttctactg ctctccttat tcttatttat aattagtttt
    35161 ctactacgtg atccagttct aataaaattt agatactctg gtttaatctt aataaactta
    35221 attggtgtaa tctggttaat agcctattta aaacatcccg aaaaaaaaca tttaatatcc
    35281 aaattttcca tacttctaat tgtcttatct cttggaattt cttctggaaa gcaattttct
    35341 ttccaattca aaaagaattt tgttgaaaat tataacaaag aagttttaga aaagtatgca
    35401 agtcatatca ttgtaggatt tagatccgaa aaagaattaa atgatcttct aaatcttccg
    35461 gttttaggct tttttataac tcatcacaat gtaaagaaat tagatttaat caaatcaaag
    35521 gaattaattg atagtattca aacaaataga aaaaaattaa attttccaaa agccttaata
    35581 gcatctgatc aagaaggagg aaatgtttca cggctttccc ctcctcttga actacaacca
    35641 agcctaggag atatgatatc ggataacgca ttactaaatg aagaattgtc taaaaaaata
    35701 aatgactatg ccgatttaca agcttcagag ttaaataaaa ttggggtaaa tttaaacttt
    35761 agtccaattc tagatttaaa gtataaaaag gaaaaaaacc cactcgattt atatagccga
    35821 atttacaaac gagcaatcgc tagtgatcca aagattgtat cctttgttgc agaaatatac
    35881 tcaaaaagac tattagaaaa taaaatcctt ccgacactca aacattttcc tggaattggc
    35941 agaataaatg aagatactca tttctttaat gcagatttaa aaacttcttt aaatgattta
    36001 gaagcaaatg atttatatcc atttctgcac attgcgcata acattgaata tccagtaatt
    36061 atgctttccc attcaactat tacagaactg gataaagaat cacctatttc aatttcagaa
    36121 aaaggaatac aagagtatat cagaactaaa tttccaatct ctacagtttt aatcacagat
    36181 gatatgaata tgggacctat tatgtataga gttgggggaa ttggcaatgc aagtgtagat
    36241 gctttaaatg caggagtaga tatattactc atatcgtatg atggtgaaca aatttatgaa
    36301 gcgttgtatg ctttatgcaa agcggatgaa tctaaaaaat taaatcaagc aagaattcta
    36361 gagagcgata aaagaataaa aagactttta tttttttctt ttccagaaat agaaagtttc
    36421 aaataaagta attagattca tgagtaaaaa ttcaattcaa ggatattaag aggaaaatat
    36481 gaaaattgta gaagataaat taaaagggaa atgggtttta atcacaggcg catcaagtgg
    36541 atttggaatt gactttgcaa atatttttgc agaaagcgga gccaatctaa ttcttgttgc
    36601 tagacgaatt gagccaatga atgaactcaa gcaaagacta gaaggattat ataaaatcaa
    36661 ggttgaagtt atttctatgg atctaagtaa aaaggaatcc ccaataaatc tctacaatac
    36721 aattcaagcc aaaaatattc aagtagatgc actcataaac aatgcaggat ttggaattta
    36781 tggagaattt gtgaatacgt cttgggagca agaagagatg caattaatgg tagacgttgt
    36841 aaatttaatg cacttaacaa aacttttttt aaaagatatg gtaaaaagaa attttggtta
    36901 tatattaaat atttcgtcta tcggtggata ccaaccttct ccactttacg cttcttactc
    36961 agctggaaaa agttatgttc taaatttcac tgaagcttta aattatgaat taagaaatac
    37021 aaatgtgaaa gtaagcacat tagcacctgg ggttactcgc actggatttc aatcgacagc
    37081 tgcaaaagat aaaccgttaa ccctatatca aaaattaagt atgatggaaa gtagacctgt
    37141 tgctgaaatt ggagtaaaag gacttatgaa aggaacacca agtgttttac caggaattat
    37201 gaataaactt agtattttct cactccgttt tacaccgaga agaatcatgg catttatggt
    37261 ataccaccta atgaggatag attaattgat ttcaactgta aatttttttt acaatcaagt
    37321 aagtaagaat agaattattt tttcgtttat cttattgagt tttttcttac ttggttgtaa
    37381 agctttatcc acaaaaaaaa aggattcggt gaatcaaact gttcatttag ttttaaattc
    37441 aacaggctat gctactgagt tttgcatttt tgaagttcaa aagcaagaaa atcaaagcta
    37501 tgaatgcaaa gcagagaaac cattagaata ttctagatgt gtagaatact tcgcagataa
    37561 tacaagttat gcaaatctaa ctcctagaga aacgttagat caaatataca aaggtctggc
    37621 tgagttggaa ctggcaagac cttctctaaa aaatagaatt aaaagcgtag gatatatttc
    37681 tagtggtagc tttaatacag caaagccaaa tgaatctaaa caagtcctaa aggaagttga
    37741 agattatttt atatctgaga agttaaacgt taaatctaaa ctattaacag gcgatgaaga
    37801 agcaaaactg actctgaaag aaattctttc taaggaaaat atttcttctg ctgcattgct
    37861 gcaaattgga acggatacta tagaagtcat aacggaaagt aatagagagt caaaagttcc
    37921 ctctgcaaaa gtaggatatc aaactttctt agaagaaatt tctactatta agccaggaat
    37981 caatgcatgc agacagccga tttcttcttt ctttaaaagt gaatcgagtt cttttgatag
    38041 atgtaaacag tttctcttgg agaatatgtc gaagccaaat aaaattagtt cgattggaaa
    38101 aacaaaattg aatcaatatg aggaggtgtt cgcaacaggc gcactcttca attattacta
    38161 cccaaacaaa aatgatttaa ctttagatga attaaatagc ataggaaggg agtattgttc
    38221 tctaactatt gatgagatac aaaaaaaagg aattaaaaag aaaaatgcat ataatctttg
    38281 ttatgcggtt tcttacacat ccacactgat tgaatatttt gagattaaga aattaaaaat
    38341 tttaccaaaa gaaatttatt taaaaaaact ggctacatct cagaccctat ttccggagtt
    38401 ttgtaaataa atgacagact tccaattgac acatgtatcc gtaatgccgg aagagattat
    38461 ctcctatttt actgaattgg gcagagaaaa tgagatacgt ttcattgatg gaactgcagg
    38521 tgaaggtggg catacccaga ttttactaga acattttcca aactctaaag ttttactaaa
    38581 tgatagagat agtataatgt tagaaagagc ttcagagaga ttgaaaaaat atttagatag
    38641 agtttttttg agagaaggta atttctctga aattctatcg aaagatattg aagaagcttt
    38701 ccagacacag aaagtaaatg gaattctact cgatttaggt atatctacct ttcatttgat
    38761 tggaagagaa aggggattta gctttaaaac caacgaaaat ctagatatga gactagatga
    38821 atcaggaaga ttgactgctt tcgatgttgt gaataaatat aaggctgaaa aattatcgca
    38881 aatcttccga gaatatggag aggagaactg gacaaagaag attgttgaga aaatcgtttt
    38941 agttagaaga aaaaaaccaa tccaaacaac acaagaattg gctaagatcg tagaatcagt
    39001 gattcctaga aaattttggc cagataaaac gcatccctct tttcgaattt ttcaggcaat
    39061 tcgtatagaa gtaaatgatg agttaggaca tataaaaaaa ggattggaag cactactcaa
    39121 tcttttagat aaagatggga ttttctgcgt gatttctttt cattctttag aagataggat
    39181 agtgaaacag gtttttagag aaaaaagcaa agatggtaag ttcgaaattt taactaaaaa
    39241 accgattctt ccaaaagaag aggaaataaa acaaaatcca tcttcccgtt ctgctaaatt
    39301 acgagtaata aaggcgttat gatagtaaca aagtttgata aaaaagaaat aaagacaacc
    39361 attagcatta tagccttagc tgtggttaaa atatccctgc ctgcaagtgt tttattttta
    39421 cttgtttggc aaagtataga ccatgctcac ttgagcagac aaattaaaaa attatcattg
    39481 aaaaaagaag agctttataa aaagaactat gagttaaaag taggaattgc cacctacaca
    39541 tcagcagaaa gaatggaatc aatctataaa cgaaacaacc aagcagctaa ctatgtgaat
    39601 aaaaaaatta gcacattgat tcttccaccg gaaaacaaaa catttgaatt ttataaataa
    39661 attaaacaat tatagaaaat tattataaca gaagcagcat gttaatttct gaattactta
    39721 aaaattttaa aaagatttcc ttaacaagcg gaaatctttc tgaaccaatc aactatattc
    39781 aatccgatag tcgaaagctt gaagaaaatg atatattttg tgtctatgat gatttctctg
    39841 ataaaacaat cgattattta aaagatgcaa aaagtaaaaa tataaaaacg atattaattc
    39901 gtaaaacgag tccctaccta gatgaaacag agcaatttcc taatgtttta atttgttccg
    39961 aagatccaat gcaattacat ggatacatag catctttttt aaaagacgat ccatctaaaa
    40021 aatgtaaaat catagctgta actggaacga atggaaaaac atccatgact tatattctct
    40081 atgatatctt ctcgagagaa aataaaaact gtggaattat tggaaccatt gaaacaaaat
    40141 atggtaacaa agtgattcaa acaggataca ctaccccaga tgctagcacc ttaaattcgg
    40201 tattagcaga tatggtgaaa gaagagattg aatatgtatt tatggaagca agttctcatg
    40261 gtctaaaact aggaaggttg aatggtctcc acatttatgg ggctttattt acaaatctaa
    40321 cgagagatca tttggatttt cacaaaacaa tgaatgatta tttaaaaagt aaattcttac
    40381 tttttaaact actatccaag tcatgtcacg aaaatacgtt tgctgccatg tctgtagatt
    40441 ccagtggctc agacaaaatg ctttccctaa tcaaaaaagc aaaattaaaa ataaaccttt
    40501 tacttctcgg aaagggaaaa caatttgaaa ccaagctaaa tgaattatcg cttaagggaa
    40561 ttgactttga ttttaaagaa aaaggaaatc agcaagttaa ctttactacg aatctattag
    40621 gtaattttaa ctttttaaat gtttcccttg cgattgtcgc ctcacgcgcg cttggtcttg
    40681 agacagaatc gattcgaaat acagttggaa acttaattcc tgtcaatgga agattccaaa
    40741 ttgtttataa tagcaaaaag actcgtgtgg ctattgtgga ttatgctcat acacctgacg
    40801 cattacaaaa tgtattaaaa agtatgcaag aaattcccca cagcaaaatc atttgtcttt
    40861 ttggatgcgg tggagataga gataaaacca aaagacctat tatgggctcc attgctgcta
    40921 aactagcaga tttagtaatc atcacatctg ataatccaag atcagaagat ccagaattga
    40981 ttttagatga aatccaagcc ggctttccta aaaaattcaa atctttttta agaattacaa
    41041 atcgaagaca ggcaattaaa aagggaattg agattttacc agatgacggt cttttactcg
    41101 tagctggaaa aggtcacgaa acagttcaga ttattggaac aaaacgagaa gatttcagtg
    41161 atgcaagaga actcattgag gctttcagcc aagatgaaat tagtaggaga taaactcttt
    41221 atgtttcaat taatatatga tctatttggt tctgatattg gtgttcttag aatttttaac
    41281 tatgttacat ttagagcact gatggctggt ctaacagcaa tggttttatc ctttgtttta
    41341 ggaaagagaa tcattgcttt tttatataaa ctcaagttta gcgaaacagt tcgaaccgat
    41401 ggtccccagt cccatgcagt caaagcggga actcctacaa tgggtgggtt aatgattatt
    41461 tccaccttga tattatctat tttactttgg ggcaatttaa aaaacttaaa cctaatccta
    41521 ctaactgttt ttagttttct attttctatg ctcggattta gagatgatta cgaaaaggca
    41581 attctcaaaa ttaaaggggg gatgaaaagc agagtaaagt ttttattatc tgttctaatt
    41641 gcttttgctt tttgttttac tttttatttt ttaacaggag aagcgcatac agcgggacgt
    41701 ggtgtttctt ttactttaac agatttattt ttacctttta tcaaaggacc tgtcattaat
    41761 ttaggaatac ttgcgattcc attttctatt ttagtaatca tcggaagttc tcatggagta
    41821 aatcttacag atggattgga tggtcttgcg actggaaccg taatgatcgc aacaattacg
    41881 tttggaatca ttgcctatgc gtcaggcacc ccgattgctg ctaactattt gaatatacca
    41941 tatttacaag gagctcatga atactctgtt ttcctttctg ccttagcagg cgccctcctc
    42001 ggttttttat ggttcaatac tcaccctgcg gaagttttta tgggagacac tggatcttta
    42061 tttctgggag ccactctagg aatgatcgca gttatgttaa aaaaggaaat tttgcttttt
    42121 atcttaggtg gaatctttgt cgcggaagcg ataagcgtta tgctacaagt cgcttctttt
    42181 aaattaactg gaaaacgtat atttaaaatg gcgccgctcc atcatcattt cgaattaatg
    42241 ggaataaagg aaacaaaaat agtaattcgt ttttggatag taggtgttat acttgcttta
    42301 ttcgcgttat ccactttgag aatacaataa gatgattcgt tttattagag aatggtttaa
    42361 aaattttact gtaatcggta aaacacctat agaccttcct cttatttata taattattat
    42421 tttgctatta gttggaattt cagctctttt ttctggttcc actgtaacag cgcacaagct
    42481 aacagaagac ccttattttt ttttaaaaaa acaagcgatt tggatgggac tttctttttt
    42541 cttttttatt gtgtttgcca ttatccccta tcgcattttt caacactatg ctgtttatct
    42601 aatagttctt tctattgtgc ttctcatttt agtatttatt ccaggtctta gcaaaactgt
    42661 aaaaacacaa gcagggagaa atttccatag atggattggt tttggtccgt ttcaattgca
    42721 accaagtgaa ttttgtaaaa tcgctgttct gatttatcta tcagctttct ttgttaagct
    42781 aagtcatatc caagaaaaaa caatcaaaac ctatttgatt ccgggtattg ccattggtct
    42841 tattctattg ttaattatgt tagagccagc gcttgggact acacttcaaa tttcaggaat
    42901 gatacttgct ctcatttttc taaatggatt tcctgtaaag aaattggtga ttgggttttt
    42961 gtccacaatt cctttgatat tatttctagt ttatagagta agctattggc gcaaaagaat
    43021 agaggtttgg ctcaatcctt acagtgataa ggatggtgat ggttttcaac tctattcctc
    43081 atttagagcc tttatggaaa gtggatggtt tggaaatcag atatcaacga gtcactcgca
    43141 caagtccctt ccatataatc atacagattt tattgtagca acctttgtcc aagattatgg
    43201 atttatcgga tttcttgtgt tactaggatt attcactttt ttaattgtaa gaggtttttc
    43261 gcttgtaaga agagtcaatg aaccatttgg attcttatta ggctcaggaa ttcttcttat
    43321 gattagcatt caaataataa taaatttatt tgtagttaca gggctatttc ctataactgg
    43381 aatcagtctt ccatttatga gttatggagg gtcttccctt ttaactatta tgatttcact
    43441 tggaatacta attaatataa caaagaggga acatatcacg tgatgcgttc tataatcatt
    43501 gcagccggtg gaacgggcgg gcatatttct ccgggaattg cactcgccga aagtttaatg
    43561 gatcaaaaac aggaattagg ttttgaaagt cttgcaattc actctttaat tcgaaataaa
    43621 gacaatcctg atttaagaga ctcgccttgt ccagttattt ggcacaatac cccacaattg
    43681 agtaattttt taacctttcc atttttattc ttaataaatt ttgtaaaaac tgtttttcag
    43741 tttagaaaat taaatgtaga ttgcgtgatt gctatgggcg gctattcctc attacccgct
    43801 ctactatatg cagtgctatt taaaaagaaa atatttttgt gtgaacaaaa tagaatcgtt
    43861 gggaaagtca ctcgtgtttt tcataaatac gcggataaaa ttgcatttag ttttccacca
    43921 gtcaatgaaa atggttttaa aaatatttct tacaaaattt tgggaaatcc acttcgaaaa
    43981 aaaataatcc cagacacaaa atccacttca aataaatcac aagtagtcaa taaaaaagaa
    44041 aagttaaatg ttttggttat gggtggttct cagggagcaa gacagattaa caatattgta
    44101 atcaactcca tgaacaatcc ggagatagca aagaatttca gctttagaat cttaacaggc
    44161 acatctcttt acgatgaggc aaaagcgaag acagaaaagt cagctgatat catttcttat
    44221 tctgaaaata tgaaaactca ttatgaatgg gcaaacttag taattgcaag atcaggggca
    44281 ggagttatat ctgaatgtgc agtgtatgga ctcccacaaa tcttaattcc ttatccttat
    44341 gctgcggaca atcaccaagt cgccaatgca gaatattttg aaaatcaagc aggagcttgg
    44401 gttttaaatc aaacggatga aaataataca aagttagttt ctatacttgt gaatattgct
    44461 aagaatagag aatccctttt gcctattaca gaaaaagcac tgagtgctgg gagggtaaat
    44521 gcctcctcag atacaattca attttttttc aaggaataat cttgcgagac aaaaaacctt
    44581 tttttattgg tattggggga tcagggatgt cctcccttgc ccatatatta cttgatttaa
    44641 aaatagacgt tagtggatac gataaagcac aatcagatac agtaaaaaaa ttggaaatgc
    44701 gcggagcgag aatcacacat gatcctgaaa atgtaaatct agatgggatt gattacatcg
    44761 tttattcgag tgcagtaaaa gaaaatcatc ctgttttcca aaaggctcta tctcttcaca
    44821 aaacactcat tcaccgatcc gagttgcttc atgaattatt ttcaaagaaa ttttcaatcg
    44881 ctgtagcagg ttcacatggg aaaactacaa caaccgcaat gactggtcag attctactag
    44941 aagctggaga gagaccatcg attatgcttg gaggcgaggt aggctattta aatgaccgag
    45001 gtggtaaatg ggataacggg aaatggggcg tttatgaatc tgacgaatca gacggaacct
    45061 ttctaaatca caaagcagat ataaaaatag ttacgaatat agacaacgac catctcgatt
    45121 tttataaaac gacagacgca ttactagaag cctttgcaaa atttattcac aataaaaaat
    45181 cttcaacctc cattgtgtat ttaggtgata aaggtgtaga agatgcgatt gatttactag
    45241 aagatcaaaa aaatattatt ggctatatag aagcttctaa agcggcaaac ctttcagcaa
    45301 aatttccaga gttgcaaata gaaccatttg aaatttttga taatgagtta agctttttta
    45361 gacaaggaga aatattaaaa ataaagcttc cgtatgctgg agatcattat cttaaaaatt
    45421 cttttgctgc cttacttgcg gcagaaactg ctgggattga agttcatgca tccattgaaa
    45481 ttctaaatgg atatactggt gttaaaagaa gattagaact tttaggaaat caaaataaca
    45541 ttcacgtcta tgatgactac ggacatcatc caacagaaat tcttgcagtg attcagtcca
    45601 ttaaaaggat gagacccgta ggggcaaaat gctgtattct atttcagcct catcgttata
    45661 ctagaacgag agatcattat gaagaatttg caaaagcctt agatgaaagt gatttgcttt
    45721 ttttgcttcc gatttattcg gcaggagaat ctccaattga gggaatttct acagaactca
    45781 tttacgatca tctagtcaac aaggaaagaa cgattctttt aacaggtgac atatcgcgtg
    45841 acgtctcaat cttaaagaaa tacattttat caggagatta tctagtgagt ctaggtgctg
    45901 gaaatgtaag aagctgggca gaagaatttt taaaatgagg ctagtatgaa cgaggaagaa
    45961 ttacacaatg taggaccaag tcttatcgga caggactggc atcatacaaa ctgttttggc
    46021 tgtggtcctg ataactcgcg tggacttcat gctgattttc ctttcgatgc tcaggcagga
    46081 gaagttcgat ttaaatttgt atttgaaaaa ttctatgaag gtgccccggg ctttgtgcat
    46141 ggtggaattc ttgcttccct tttagatgaa gcacaaggtg ttatgtgttt tcatgcaggt
    46201 cattttgtaa tgacagatca actttttgta aaatacgata aagcatgtcc tcttgaagaa
    46261 gaagtggaat gcagagcttg gatcacaatc gctaaaaata gaaggcttta cacaaaggca
    46321 accattcact ctccaacgac aggcgaaata tatgctcatt caaaagcccg ttggtataat
    46381 atgaaagaaa aagtttttat gaaaatgttt catcgttctc ctcttcgcat ggaaagacta
    46441 aaacacattc tagatttaaa taagaaaaga ggtaaagaaa ttagaaaaaa aattaaatca
    46501 caaaaacatt cttgagaaaa tgaattattt ttactactga ttcgcataca taaaagattc
    46561 gatcattcat caatgcaatc cttcaaaggc atccgtgatc aaaccctttg ttccaagtat
    46621 tcaacactaa tgcctccgca tgtttatcag aggtgaattc tttataaaat ctctatatta
    46681 gcaaagtttt tcatcttttc tatgttgcct aacgcaatag ctagactatc tatattaaat
    46741 tcatctgcct gttttaatct tgaggtttga gactcgcttt ccaaaagttt tgcagttttt
    46801 tctaattgaa ttccagcaag taagcgtgtg gcaacctcta ctagttcgag cgaaaaactg
    46861 tctctcactt ctgcactaga aatattttta tataccgaaa ctgcttcttc taatttttca
    46921 aagagagttt ttaatcttga actcggagaa aactcttcca tctcagaaag tagatatttt
    46981 ctaaaatgcc ctgtgggagc cataccagaa atgactccac cgatagccgc cataacttga
    47041 atctgcgtag ttccatcata gattgtaatt attcttgcat ctctatagat tcttgcaatg
    47101 tcataatctt ctgtaaaccc agaacctccg tggatttgta aggcatcata ggcaattcga
    47161 ttgcacattt cagaacaata gtattttgaa acaggagtaa gtaagtcggc gagcttcatc
    47221 cagtgtcgca cttcctcatc atttgaaatt tctttatcag atttccctgc ttcttttaga
    47281 tgttccattc tccaataata catgtctacg gttcgaccac cttccaatgt gagacatcgc
    47341 atcgccataa tttctctctc catacgacga agcatttttt taattgcggg aatttcctcg
    47401 attggttttc caaattggat tcgagttgag gcaaagattt tagcttcttc atatgcggca
    47461 gtcgccatac ctactccttg agctgcaatt cccattctag ctccattgag cattcccatt
    47521 gtatatcgaa ctaaaccaaa tccttcctcc ccaatgagaa gagccggagt gttttcaaat
    47581 actacttcac aagtaggaga agcatgtaga cctagttttt tctctattcc ggctacttga
    47641 atatctttac tatgaaccaa aaagaaagaa agtcccctag caccgtttcc gccacttcta
    47701 gcaagcgtta gtataataga aggagtttct ccaagtccac aggcttgtgt aataaagcgc
    47761 ttggttccat ttaacaccca tttaccgtct tcttgcttag ttgcttttgt ttttatattg
    47821 ggtaggtcag atccatgatt cggttctgta agccccattg cagcacagaa ctcccctgct
    47881 gccattctag gaagccaatc ttttttcatt tcttccgatg caattctctc taaaatttct
    47941 ccaaggttca cacaaccaaa tgcaatagcg aaacttgtat ctactctata aatgatttct
    48001 cccataagag ctcgaacagt aaaaggcaat ccaagtcctc catattttct ttggaagcca
    48061 taggcttgga ttccagattc cttggcttta ttcactgaat ctatcatttc ttttggatga
    48121 attactttac cattttcata ctttgctcct tggatatcca tggatttcac atgttccgaa
    48181 acaaaattac ctgcaatgtc acctgccgtt tctaaaatag ttctatagta ttcaacggct
    48241 tcttcgacac ttgtaggtgc cgatgctaat aattcatttc cagtttgttg aaacaccttc
    48301 gcatcatgaa aattattttc ataaattgga actatctgtt tccaagggat aaatttttca
    48361 aagtgaagta gtatatctga attagtagta aagtaatttg actttatcat ttctttttcc
    48421 tttcttcaat tattttcaat ttacttttac ttgtaaatac tttttaaaaa taaaataata
    48481 ttttatatat actgttcagt atagtatatg tggataatat ttgttattcg atatttaaat
    48541 aggatagatt ttcttttttt taaactaaac attactcatt tagagttcct gttttttgtc
    48601 ttcacactga acccagaatt agaaatagat aaataattct ttttacttct aaatataaat
    48661 ttataaatta aaaatattcc tttaataata tttttacaca attatatcgt tttctttcta
    48721 caagaaggct aaattctcta ttttccattt ttatcttttt taggtttatt tcttattttc
    48781 agtttagtag atttgctttt agaaagtttt taatactaaa tgtttgaaat tggtgtgatt
    48841 ttttctgatt tagttttcaa tcttagtttg ggttttattg agaaactttt tttaaaaaca
    48901 tctataattt accaaatcag taggtttact cctataaaat aatacttata aaagctattt
    48961 ataattcgtt tctattccaa ttatcgcaat tatttagaaa aatagaatag ataaaattct
    49021 tcttttcttt ttttatggtt gaaactaagg gagatttcaa cattgaaaga atttaattta
    49081 aaacaaatac aagacgttat accaaactca aaaatactaa atacggataa cccagaagcc
    49141 attattttta attcacttgc accattaaat tctgacaagg aaaaattcat ttccttcgta
    49201 tcagcaaaag gccatttatc ggaagcaaaa aagacaaaag cagtagccct gattgtaaac
    49261 caagagattg cttctaattt tacagatatt ccaattctta tagtagacaa tgtagagtta
    49321 caaaatgcta aaattttaaa tttactattt cctcccaaaa ctccgaatgg agtcatctct
    49381 acaacagcaa tcattcatcc gtcagcaaag gtaggttcga atactcaact agggcattat
    49441 gttgttattg gtgaaaattc tgaaattggt caaaattgta ttattgaagc aggtgtaaaa
    49501 ataggcgaca atgttaagat aggaaacaac gtacgaattg gaatgaattg cgtcttccat
    49561 gatggaactc agatcggaga taattttatt gtatttggaa attctacctt tggcggagat
    49621 gggtttaaat ttgtagcttc aaagggtatt catcacaaga ttccacaagt tggaaaagtc
    49681 attattggaa atgatgtaga aattggatgc aactgcactg ttgatagagg tggactcact
    49741 gacactataa ttggaaatgg ttgtaaattt gacaatatgg tgcatatagg gcataacgtt
    49801 gttataggcg atcatgtgat tattgctggt caatctgggg tagcaggaag cactacgata
    49861 ggtaacggag tcatcattgg aggtgggtgt tgtattagtg accatttgat ccttccggaa
    49921 ggaaccattg tagccggagg cactggaatg cgaacttctc caaagaagcc agacgtatat
    49981 attggttggg actggggaat gacgtttgca gaatttcaaa aagcaagagt aaatattaaa
    50041 cacttagtta cttttaataa atgggcatct cgtattaaag aactagaaaa aaaattagga
    50101 attaaagttg aggaatagat aaagactatt cctctttgaa aatcaaagca gaagatgtag
    50161 cggttgctat taatttattt ttctgatcat acgcttctgc tttcataaaa attactttcc
    50221 ttcctctaga cgttacttct gctcgaatgg tgacagtatc cccaacctta acacttctca
    50281 aaaaatatac atgaatgtca atactcacca ttggcttttt taaaacagca aaacttagag
    50341 gtccaaatgt atcatcaaag aaggcagtca atattccacc ttgtaggatg ccaagaggtc
    50401 ctgtatgttt ttcttgcact ggaaactgaa cagctatact ttttttgggc acatattcaa
    50461 tgatcttgga ttgtaactcc aatgcggaat tcggtggtaa tttaattcca aaatcatcac
    50521 gcattccacc actcttacca aatatttgtt cctttaaatc tttttctaaa tcttccatgt
    50581 attcctcaaa aatgattttt tatttcagtg ttatcttgta agttattttt ttctgattaa
    50641 aacattctaa actaaataaa ttagtaaatt tagtttaatg agagaaaaag atgcaattaa
    50701 aaatgtttta ttattagaat ctgataaatt ttgatcttag ttttaaatgc cgatattatt
    50761 tcatgttatt atcattttta acaattcact tagtatctaa aaactgtaac tgtatctgaa
    50821 aattccaata ttatatcaaa ccagctaggt aaaacagctc attctgattg aaaaatcaca
    50881 atcgttggat atcctcaatt agaattcatc taccaaaact cattcactgc ttttctttaa
    50941 cattgatact ttaaggtgtg taaaaaatta aaatgaaaaa tcaaattatt tcttttagat
    51001 aatacttgaa tctattgttt taattccatt tgtgttatat atgaaatata tagctttaat
    51061 ctttttattc ctagtctact cctgttctaa aaccaatcta gaaaaaataa accaatactt
    51121 agaaaaaggg gattatgaga gattactgga attcgcaaat aaaaataaat caaattttaa
    51181 agaagaagaa ttgtatttaa gttcggttgc agtttaccaa ttacaaaact cacttagaaa
    51241 aaataaatct gatgaaaata tggtaaactc aaactacttt caaaaattag aaaaaaaaat
    51301 aggtttaaaa attaaaactg ttacaacaga taacagtgtt ttgcctgtaa tcgatgatgt
    51361 ctatgaaaaa ctgataccaa aaaattcgga ttatcgaatt aaaatccttt tagatcggtt
    51421 taaatataca tataaaacaa atgatcctac tgaaaatact tttctgcttt tagatatatt
    51481 ggagcaggat ccaagacagt ttcttcaaga atataaattt atatggatag aagcaatgaa
    51541 gcttcaactc ccgaagcttt taccagatga atctaaaacc aaattcaatc attttctaca
    51601 ttacttagca agcaaagaag acacagatat aaaaaatcat ttcttcgtta ctgtggggac
    51661 taacgtgaat ttaagatcag gaccagggac agaaaatgca aatataggaa aattaaatca
    51721 agaagaagtc tttcagattg attcagattt taatacggtc aacattggaa acaaaacagg
    51781 caaatgggtg caaatatata tctggaagag tgatttagct ggttggattt tttccccttt
    51841 tcttaaaaat ataagctgga atcagaaaat agcagatgaa tttgaaagag attttattca
    51901 aaaaaataat ttcagtatga ttgattttaa taattggaat ccagaggaaa taccaaatgg
    51961 tttttatggt aattatattc ctagaaataa ggaaattatt gaggctaata ttggttttcc
    52021 agtctactcg tcaaaaacag aagaagcaat ctgtaaaaaa ctaaaaaaaa tcccaaacaa
    52081 attattgatt catttttata acaaagatac gaaagaagaa ttaaccttgt tttacttaaa
    52141 atcaatcctt gattcaggag aaaaaagaaa gaccttagta aagataattg accgaaaatt
    52201 agttttaaat caaagatcaa ctgaaattga gttggaaaaa aatacagaac agttcattga
    52261 aatcaaaatt gataatgagg aagaaaatac aaaactttct ctgatttcca tgaataatag
    52321 agagtatttt gatttattag caagaaaggg agaaatcatt aaatcctgtg aaatctgcat
    52381 tcctcaatca aagaaaaatt caaatgtggc atttttattt tcttttaaaa tatattaaca
    52441 gtgttttgca aattgttttc tccttgcaat aggtctaatt tttaaaatct tagattaaag
    52501 gataaaaacg tgaagaaaat ttatttggca aatcctagag gcttttgtgc tggagttaag
    52561 tatgcaatct cttttgtaga aaatgttcat aatttatatc aaaatgaaca aatctacgtc
    52621 cgcaaagaaa tcgttcataa ccgtagagtt gtaaatgata tggagaaaaa aggaattaaa
    52681 ttcataaacg atctagatga agcaccagaa aattcaattg taattttcag tgcacatgga
    52741 gtatcaccga ctgtagttca gaccgctaaa gataaaaata tgcgcattgg ggatgcaacc
    52801 tgtcctcttg taacacgtgt gcataggaaa gctaaaaaat taaaagaaac ccatcaaatt
    52861 atatatatag gtcatgaggg tcatgatgaa gcgataggca ctatgggtga ggcagaaatg
    52921 tttttagtag aatcagaaga agatgtagaa aaactattac ctagaattga cccaaacaga
    52981 ccactcacat atctaatgca aactacccta tctgtaagtg atacaaagaa agtaatagat
    53041 aaaattgcag aagtatttcc aaatgttgaa catcctgata aagacgatat atgctatgct
    53101 acaacagaaa gacaggaagc agttgactct atgatggatt ccgtagacgc aatgcttgtt
    53161 ataggagcac ccaatagttc caactcgatg agacttgttc aattggcaca gaagaaaaag
    53221 ccaaaatctt ttagagtcag ctctgctgat gaaatcaata aggaatattt agatagttta
    53281 gaaattgaga ctatgggaat tacagcggga gcttcctctc cacaggtgtt aattgatgag
    53341 attattaata aaataaaaga attttatcca gaaattaagg tagaaaactt ccctacttct
    53401 agagaagatt caatgaattt taaattacca aaagagctat tgaaataact tattcaacag
    53461 tagatttagt tttaatcttt tgttttaatt tacttatttt atttcttaga ttagatattc
    53521 tcttttcata cttatcaatc ttttgctcta agattgttac ttttttagtg atatcctgtt
    53581 tatcatcttc catttcatct aaagatgcta gctcttcttc atcgtctggt ttattactct
    53641 cagatttctt ttgtagtttt gcttgctttt ttttctcttg caatactttc tgtagctcgg
    53701 cagaattcga agagaagatt actttgtgaa cagtgctttt tggtattacc tttgtagcac
    53761 cctctgtatt tatagttact gattctgaat catgccccaa aactttccct tgaatgattt
    53821 gtccattacg tagtaaaatg gtttctgctg cgagagatcc aattagattt atataaataa
    53881 aagataagat tgcaaattta aatttcatga tgtgtattcc aaaacgttta attgtacctt
    53941 tgattccatt ttcgacatag aaatatttta attcaagtag ttttattttg aattcatttt
    54001 atttatacta tgtagtagac accaaagtta attagataaa agaattgaaa ctcaatggaa
    54061 tgaaagactc tattcaggaa atgcgtcttt tgcctctaat tttcctaaat aattccagtg
    54121 ccccatcttc cttcctttct tcggttcctc tttctggtat aaatgtaatt tatattcact
    54181 ctttttaagt cgttcgattg atgtctcttt caaatccata aaatcaaaac ctattatatt
    54241 tttcatgaca cacgggtttg tttttatttc ttccatgtca ggttctagat tagtcagagt
    54301 atgcaattgc atttcgaatt gagaaaaatt agaggcatcc attgaaaaat gaccagagtt
    54361 gtgaggtctt ggggcaaact cattacagat gagttgagtt tcttttacaa aaaattctaa
    54421 agccaagact cctacatagt ttaaagaact taataaattc ttaacggctt gaattgccgc
    54481 ttccgttaat tcatgattga ttgaagcagg atggatagag atatctaata tatgattttt
    54541 atgagaattt tcagatggaa gataatacaa aattttacca ctatcaaatc gtcccgcaac
    54601 aatagaaatc tcacaagaaa aatctacaat cttttcaatt atgtaatcaa tacttttaga
    54661 ttgctttatg attttctcta aatccgaaaa gtcttttatt ttatgttgac cttttccatc
    54721 gtatccaaat tggttcgtct ttaaaataca gggatattct attttatttt tattttcata
    54781 tagagattcc aaagaattta gatatatata atcagtagta tttaaaccaa tctttctaaa
    54841 attttctttc tctttaaaac gattctgtga aattcgaatt gaatttacgg aaggacaaac
    54901 ccttagattt ttattttgaa catactcctc tatcacagaa agcgctgctt caggtatatt
    54961 ttcaaattca aatgttagag catctatatt atccaaaaac tctcgaatac taactttatc
    55021 agtataatct ccaacatatt ctatacaacc agctttaaat gcaggggaat ttttctcagg
    55081 agaatagcaa ctaacgctat atcctctctc cttagccttc tgtgcaaaca ttctaccaag
    55141 ttgtccgctc cccattacac caagctttga ctcggatggt aaaaataatt tttctgttcg
    55201 tttcaaatta gatccttttc ttttgcaaga gcagcttctt taatttcctt actatatttc
    55261 tctaattcga ttcttaattt atcattttca ctaatgttct ccattaaatg atatataatt
    55321 aat
//
LOCUS       DAOQOS010000020        55150 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_220_length_55150_cov_5.523296, whole genome shotgun sequence.
ACCESSION   DAOQOS010000020 DAOQOS010000000
VERSION     DAOQOS010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 55150)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 55150)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55150
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_220_length_55150_cov_5.523296"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..640)
                     /locus_tag="PLF66_06260"
     CDS             complement(<1..640)
                     /locus_tag="PLF66_06260"
                     /inference="COORDINATES: protein motif:HMM:NF012509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HMZ63681.1"
                     /translation="MNHKIYIRVPGTSANLGPGFDLLGMALKIYNEFYFTIDPNSNFT
                     TLRIDNTPLPFSDKEDLLLEGYKKYFDLFLHKQKLIAYNVKMDLNLPFKGGLGSSASA
                     LTAGFTLGHYLHKKHFSKKFPVPNLDKILYELALLEGHPDNTTPAMIGGFVFSYFHEG
                     KLISFKEKFPSSISLFLFIPQIEVSTNDSRKKLPDQYPVKDVIFNMSRMASWM"
     gene            complement(637..1173)
                     /gene="pth"
                     /locus_tag="PLF66_06265"
     CDS             complement(637..1173)
                     /gene="pth"
                     /locus_tag="PLF66_06265"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017850237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HMZ63682.1"
                     /translation="MSGKLLIAGLGNPGDKYNGTRHSIGFAVLDKLSDKHGISFNSDK
                     KCPSTDLFLFDKKIILIKPFEFMNLSGNGVSLFMRKNGINPSELLVVHDEIDFEFAKC
                     KMKIGGGHAGHNGLRDIIAKIGSADFHRLRVGVGKPKDGREVADYVLSRFSSEEKDKL
                     ESVYNLCIDKIQDWIKTT"
     gene            complement(1170..2702)
                     /locus_tag="PLF66_06270"
     CDS             complement(1170..2702)
                     /locus_tag="PLF66_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016547841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-isopropylmalate synthase regulatory
                     domain-containing protein"
                     /protein_id="HMZ63683.1"
                     /translation="MSNTKLEILDVTLRDGEQTQGVVFSSQEKLNIAKFLLLNTKVDR
                     VEVASARVSRGEKETVRHIMNWAEKENMTNQIEILGFVDKNASVDWILESGAKTLNLL
                     TKGSEKHLIHQLKKTAEAHLDDIKATIDYALSKGITVNVYMEDWSNGYLNSPDYVAKM
                     AKAISDFPIKRLFLPDTLGVLSPDETTKAIRFMREIVKNKPIEFHGHNDYDLAVANCL
                     AAVNAGVSGLHVSVNGLGERAGNSPLEAVITAIHDKTAFRTNIVESEITALSRLVEVF
                     SGKRISDNRPIVGQDVFTQTAGVHADGDKKGNLYANAILPERFGRKRSYALGKLAGKA
                     SISENLKQLGLVLSSDLEKKILDKVIEMGDQGIIVSTEDLPFLIANLSGEDLNTNFQI
                     LRCEVLSGKGISPTAKLSIKYKAKESYAEGKGDGGYDAFMDALRKIAKENNFTLPELL
                     DYQVRIPPGGKTSALVETIITWSGNVHNSQTNSFRTIGLDSDQISAAILATEKMLNLI
                     LK"
     gene            complement(2699..2974)
                     /locus_tag="PLF66_06275"
     CDS             complement(2699..2974)
                     /locus_tag="PLF66_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002136704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HMZ63684.1"
                     /translation="MIEIYIRKMRYEEAKQKLERELQDAFMRGETLVQVVHGIGEGKL
                     KELTLETIRNYDYLRLVNTEQFITPNPGVTKIEIMGLDKQDLKKYIR"
     gene            3696..4184
                     /locus_tag="PLF66_06280"
     CDS             3696..4184
                     /locus_tag="PLF66_06280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63685.1"
                     /translation="MNENSILEYFQAEKMESLLFMILGGIGILAAIFVFIYSIQYKNI
                     IYPFIVIGLIQIIVGGSVFFRTDKQVESLLIQLSQNKIEFKKSETNRMQIVIKNFEYY
                     KALEIFLIFLGIVLSYLYPARVGIYSIAIGLILESSLMLVLDLFAEKRAIDYLQWIQN
                     LN"
     gene            4204..5826
                     /locus_tag="PLF66_06285"
     CDS             4204..5826
                     /locus_tag="PLF66_06285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HMZ63686.1"
                     /translation="MNLERKYLAFNSIVSSTQNILYIYNAEKKEIEFLNPMAAVQVDI
                     IKKEESKISIENFIEKIHPQDRLKYLSQIDGVLSGREKKEKPIEYRLLRKNGDVFWIS
                     DTLILLKIEDAESKEIVGFASNITNVRRKEEKYQNEEIRLRAILENAREQYYFIGVDY
                     KVQSLNKNAKDFLIKLYNRNIFEGYSILEFLPLENPNTFTEKFNYALNGTAIQYETQI
                     KDEKGETNWFQFSYVPVRNKTNQITNICFIIVDITEIRKATKNLMDLNQELEDRVKER
                     TRELLEEVAQRRITESLLQKSLIKEKELNELKSKFIAMVSHQFKTPMTTISMSTQMLE
                     NYFEKQSEVKRRRHYSKIKEATQSLNRLIEGILSVSRSESSSMPFDPSKVDVPTFLHE
                     LIGNFQSNHPDYRFEIRFEKKNKTEYNIDSNIISHILENLLTNAVKYSPVNTTIYITA
                     VLEEKSLLFIVKDNGLGIPKEDQDRIFETFYRGKNVANISGTGLGLNIVKNLVELHLG
                     KINFKSSESEGTEFLIWIPDIVENVDGNNFNH"
     gene            5804..6487
                     /locus_tag="PLF66_06290"
     CDS             5804..6487
                     /locus_tag="PLF66_06290"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HMZ63687.1"
                     /translation="METILIIEDDDLIRENMVELFINEGYHTIAASNGRLGLEEAKNK
                     ILDLIISDIMMPEMDGIEVYKNIRSEPILSAIPFLFVSALSEISNVRSGMVLGVDDYI
                     TKPFLNSELITVVKNRIEKSRNLRKSLEDLKSNLIRSVPHEFLTPLNAVIGFSQLLMD
                     GCGELHKDEIYEYSHYIFSAGQRLLRITRNYILFTDLTMNEKEYKTRASLENEIYENI
                     EYFLSEYLY"
     gene            6551..6892
                     /locus_tag="PLF66_06295"
     CDS             6551..6892
                     /locus_tag="PLF66_06295"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HMZ63688.1"
                     /translation="MSDKNLRKILEELLDNAIKFSKKNINITGQYVNGEYEIMIQDDG
                     DGISMTNLQNIEAFMQFDRKMLEQQGLGLGLAIVKKLSSISKCRFHIESVVGQGTKAF
                     LYIPLAKNEQG"
     gene            7050..8300
                     /locus_tag="PLF66_06300"
     CDS             7050..8300
                     /locus_tag="PLF66_06300"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HMZ63689.1"
                     /translation="MNDKIKIPRYPVLIVEDKKENQVLIQSICNQIGIQTEIAGNGHI
                     ALNLVKENKFSIFIVDLMLPVLDGKSFIEELKIIEPNAVVIIQSALDSSETIIEVMRL
                     GVFDYVIKPIDIDNFRHTLSKALEFKHLKDLEAELILNESIKLRGQLEWLNYKETLRL
                     TGKDSYQKNSIFNLTTSLSQGGGVGVTISLLDMLKMDMEEQGDRYIVNKEILDTLFEN
                     NEYSRRVIQGLGSVVDLMEQPFQFSLSSASSFIKEFPSFFKHIIPYMQNKNITVAYPS
                     VQSTVQIEIDIEKIKLVLEELLINAYKYSVSSSRIDIFAHITGTYFCITFKNEIDENS
                     YGGIPPEKEKLVLEPFFRIHPPVEDILAVEKFSLGLGLTVVDFIINKHNGMFFIHNAV
                     DHTGKKRTNCVLAEVFLPIKLERS"
     gene            8303..8668
                     /locus_tag="PLF66_06305"
     CDS             8303..8668
                     /locus_tag="PLF66_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002973983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HMZ63690.1"
                     /translation="MSKRILIIDDSAVFRKVVSMNLKHAGYEVIEAVDGLDAFNKLPT
                     IKPNLIVCDVNMPNMDGLTFVTKVRADENNRFIPIIMLTTESQEEKKKKGMEVGAKAW
                     LVKPFAPEQLLSAISKLIA"
     gene            8671..9042
                     /locus_tag="PLF66_06310"
     CDS             8671..9042
                     /locus_tag="PLF66_06310"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HMZ63691.1"
                     /translation="MSVEIEITNHSNHCKVTLVGKCTIYNTIKLKDTLLSVIQSNENI
                     LIDLAKVNDFDTAGMQLFISAKNTAKKYNKKIKFVSHPDCVLAIMDLYGLVGFFGDKI
                     VLSQDGRKKFPFKYGTKKRNA"
     gene            9046..11226
                     /locus_tag="PLF66_06315"
     CDS             9046..11226
                     /locus_tag="PLF66_06315"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HMZ63692.1"
                     /translation="MNANLDEVKELFISESSEILQKMESILLFLEMNPVTDDKIHELF
                     RSIHTIKGSAGIFGYEETVSFTHVVENLLDKVREKEIQLEKPLISLLLDCRDHISDLI
                     EYAANGRSLEEQMRSVQNDLLVKLNSYLSNFKTTTKEKDTPAKESVTPIPLSNPNANW
                     HISLRFKQNTFRDGLEPFSVLKYLNKDGEILNLVTLTNLIPDLENLQAEDCYTGFEIE
                     YKSDKDMSFILQAFEFVEYDCAISILPPDRTCQDIYNFLIDPNYKTKRLLRILLEMGA
                     MTRDELHQMNQWKRNKTSNTTLNESLPTVEDESREIQQIENIETYVENKQVKQTKESN
                     RENKIIRIDSSKLDHLINLVGELVIGGANINQLSQKRGDPELSESAYFMNHLIGEIRE
                     TALKIRMVQIGETFNRFQRTVREIGHELGKEIQLTISGGETELDKTVVEKINDPLVHL
                     IRNAIDHGIEPKEERVAKGKPAVGQLTLNAFHETGSIVIEVKDDGKGLNREKILNKAQ
                     EKGLVSGDKQLSDSEIFRLIFKAGFSTADKITNISGRGVGLDVVEKNIDALRGSVQVF
                     SEQDKGTTFKVRFPLTLAIIDGFLFRVGKSFYVVPLDLVVECLEYKEEESVDGSKDYI
                     NLRGEVLPFIRLSEFFQTEEESNTRKNILVIQYAGKHAGLLIGTLHGEIQTVIKPLGK
                     VFEKLKGISGSTILGSGEVGLIIDIPSLFKKVEEIERKFVSGRN"
     gene            11260..14481
                     /locus_tag="PLF66_06320"
     CDS             11260..14481
                     /locus_tag="PLF66_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HMZ63693.1"
                     /translation="MLIRNISIQAKLVLLYFLAFFCSGLLISFFGIGISDFESKINTF
                     INNQNKTNIEFKDLQIEVNESTFLLSEMILSQSPEKAKSIAQNLELKNDLVITKLETL
                     QKQVQDPSIKEELLNLKNNFDLYHKAANEVKSLAMESKFIEAESLYQKNINSLLIKVK
                     TNEKELNQLFEKSFKENSENTMTGFLHQKSDLTTKSVLFVSVILLLMGFILFGVVFTI
                     KSAHNSFKRIHSGDFKEQLNEIGKDEIAQISKTFNTFYSSYSAINRSQAVIEFSIDGT
                     VLSANDNFLTAMGYTLNEIKGQHHRMFVDSAFSLSEGYSQFWASLARGEFQSGEFKRI
                     GKGGKEVWLQASYNPIFDSKGRPFKVVKFASVVTEQKRKNLEYEGQIAAIAKSQAVIE
                     FTMDGLIVSANDNFLNSMGYTLSEIKGQHHRMFVDKNFSLSEDYRQFWLALGKGEYQS
                     GEFKRIGKGGKEVWLQASYTPILDLSGKPIKVIKFASLITEQKQKNLEYEGQIAAIGK
                     SQAVIEFTMDGLIVSANDNFLNSMGYTLSEIKGQHHRMFVDSAYANSEEYRQFWNSLT
                     RGEYQNGEFKRIGKNGKEVWLQATYNPILDLSGRPFKVVKFALEVTKQKLKNVEYEGQ
                     IRAIDKSQALIEFRMDGTILTANDNFLAAMGYNLNEIKDQHHKLFVDSSYAASEDYRE
                     FWASLNRGEFRAAEYKRLAKGGREVWLQATYSPILDLNGKPYKVIKFATVITEQKILA
                     NETARIVSDLIPSLAALEKGDLTNLIQSEYEGSFAKLKDSFNNTLNTLQGIIEEVSSS
                     TDAVVSAAEEVSSTAQSLSQSASEQAANVEETSASLEQMSANINQNADNAKQTNTIAT
                     KAATDAVQGGASVLETVKAMKQIAQKIGIVEDIAYQTNLLALNAAIEAARAGEHGKGF
                     AVVASEVRKLAERSQFAANEISDLASSSVQIAENAGKLISDIVPSIHKTADLVQEIAA
                     SSAEQASGVGQINKAITQLDSVTQQNASASEELASTSEELSGQAEALRLAMTFFKTGK
                     ESQKEIKPEKVSSVKKTIAPKTNVVNKKSKPIPVESSHDFEKF"
     gene            14493..14990
                     /locus_tag="PLF66_06325"
     CDS             14493..14990
                     /locus_tag="PLF66_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012388567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HMZ63694.1"
                     /translation="MQSKVIEESQYLTFLSGSEVFGIGILKIKEIKEYSSVTTIPMMP
                     EYVKGVINLRGNVVPIIDLLVRFGRGKSTISKRSCVIIVEVEHEEESIDIGILVDAVN
                     EVVDIPPTSIEPAPSFGAKIRIDFIQGIGKLENQFVILLNVNKVLSISELSSIEEKLT
                     SDINN"
     gene            15012..17924
                     /locus_tag="PLF66_06330"
     CDS             15012..17924
                     /locus_tag="PLF66_06330"
                     /inference="COORDINATES: protein motif:HMM:NF024140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HMZ63695.1"
                     /translation="MQNTNNQETKFSIATIGFVVILLLITSIVFYQIDRLGKLNDQLI
                     EQDMQAVSILKDALSTARDNAIATAEYFLDSDKGKHAILSERIKKNRSTIDKKLETLD
                     RLILDSENKDKLNKLVTNRKAYIAISNEIVDLITNKNDIKTATYKYTNELNPKLERYM
                     EIYAELMLGLDKITKQNQEANKRALSLAQTILLSFNLIIIGIVTFGWFWIRGILRNIQ
                     KSSIENLKFRVALDCTSTNLMMADNDRVITYMNKSIKKMFAKAEADLKKQYPNFNLDN
                     MIGTNIDTFHKNPSHQKGLLTRISEEYKSVIEIGERKFTLLANPIIDSNGVRYGTAVE
                     WSDITAELAAKAELEKTMNENLQIRVALDNVTTNVMMADNDRNIVYMNKAIVSMFTNA
                     ESDIRKQFPSFSAANIMGKNIDIFHKNPAHQKQILNTFSSTYNATINIGGRTFTLAAN
                     PVVNEKGERLGSVVEWKDITSELLAKAELEKTMNENLQIRVALDHVTTNVMMADKNRN
                     IVYMNKSIYNMFHNAEMDIRKQFPGFDAKNLIGKSIDSFHRNPMHQQQILGTIATTHN
                     ASIQIAGRSFSLSANPVINDKGERLGSVVEWQDITSQLAIQKEIDDIITGAAKGDFSK
                     RISSVGKEGFYKQLTEGMNRLMEVSSIGLEEVVRVLGALEKGDLNEKITNDYEGTFGK
                     LKDYTNNTVAKLTEIISEVRRSTDALVSASEEVSSTAQSLSQSSSEQAASVEETSASL
                     EQMNANINQNADNAKQTNAIATKAATDAVQGGNSVLETVKAMQQIAQKIGIVEDIAYQ
                     TNLLALNAAIEAARAGEHGKGFAVVASEVRKLAERSQVAANEISELASSSVQIAETAG
                     KLISEIVPSIHKTADLVQEIAASSSEQASGVNQINKAITQLDSVTQQNASASEELAST
                     SEELSGQAEALRLAMTFFKLDELETYAPSQKKTTQIKKMQKTTRLVERDSSDFEKF"
     gene            18013..18825
                     /locus_tag="PLF66_06335"
     CDS             18013..18825
                     /locus_tag="PLF66_06335"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HMZ63696.1"
                     /translation="MNIPPLLDKEFKYFQKLIFELAGLNMTIAKKPLIGNRLATRLRY
                     YELDSYLEYYEIVADSRNKSERQIFVDLLTTNETSFFREPIHFDFMKSQILPKLETQN
                     PFRVWSAACSSGEEPYSIAMLLDEFIKNNSKWEIIASDISSRVLEKSKKGLYPIDKSN
                     MIPERYLKQYCLKGVNSNEGYFKISDYIRNKIEFKSVNLNEHFPKDLGKFNLVFLRNV
                     MIYFDKPTRINLIRKISEILQPGGYLFIGHSETLLEITDALMMVRPTIYKKK"
     gene            18822..19319
                     /locus_tag="PLF66_06340"
     CDS             18822..19319
                     /locus_tag="PLF66_06340"
                     /inference="COORDINATES: protein motif:HMM:NF015908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HMZ63697.1"
                     /translation="MNIPEHILEIFLMPGDFFWGDSSTRIRTVLGSCISICMWHPVKR
                     IGGMCHYMLPTRNVDFLRENIRLNGKYGDEAWEMFAREIRKANTKPSEYIVKLFGGSN
                     MFLSRDADVPDIGVGNRNIELARKIISDFQLNLVSENLGGNKPRRIHFDIWSGNVWLK
                     RQENE"
     gene            19320..20393
                     /locus_tag="PLF66_06345"
     CDS             19320..20393
                     /locus_tag="PLF66_06345"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015682630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HMZ63698.1"
                     /translation="MNKIQIAVIDDSALVRQVFSEIIQEESDFNLLFTASDPLFALDK
                     MKKAWPDVIILDVEMPRMDGITFLRKIMQERPTPIIICSTLTQTNSPTLMQALQNGAV
                     DVITKPSVGLKDFLSDSKRLFIDAIRAASVSNLKKLNIKTLQTGTKTFSDIDITKTKV
                     VPVTQLTTTEKIIAIGTSTGGTIALEFILTQLEANCPGIVIVQHMPEKFTAAFADRLN
                     KLSKIEVREASDRDRVLPGLALIAPGNRHMSLKRSGAQYHVEVSDGPLVSRHRPSVDV
                     LFRSVAKHASSNAVGIIMTGMGDDGSNGLKEMHDQGAFTIAQNEETCVVFGMPKEAIK
                     KNAVDKIVGLEEIPNIIMKYRTV"
     gene            20390..20938
                     /locus_tag="PLF66_06350"
     CDS             20390..20938
                     /locus_tag="PLF66_06350"
                     /inference="COORDINATES: protein motif:HMM:NF025743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4416 family protein"
                     /protein_id="HMZ63699.1"
                     /translation="MNKSIYRKEEIIRPEGSLPFIIVSYTLEDVYYKIKDNLTKIYSD
                     VLFESIQLAGWTPSVSELGFHPPGNKSKILSFKRKVHRDELPEIKEKCIQIANQNLKK
                     DQSIRMTPGYQTLFNTVTASITDDFHKVYLFKGVFGEVVYKYESRQLQYIDTAPLFFK
                     DSDVVYYFTNLREYFNQLNQIK"
     gene            20949..21509
                     /locus_tag="PLF66_06355"
     CDS             20949..21509
                     /locus_tag="PLF66_06355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63700.1"
                     /translation="MKKNIFFKLIVLTYYCLVLYNCKPQELKIENIELCENFASDGVC
                     REKLDNSHTYEIKINKNKKYETWEALSNYMYFHSRQTPGFIVRFNRKFTLTERETLHK
                     TYKAKYEFYGSSGIVEGFEIGDDWIGSFQYLGSIVKDRQRHFKEEKNFPYLETVFPAS
                     IRFSFDSALAKGQAAVEVNVRLNYIE"
     gene            21514..23112
                     /locus_tag="PLF66_06360"
     CDS             21514..23112
                     /locus_tag="PLF66_06360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63701.1"
                     /translation="MKVIPRLNNILLVFFLISSTAFLVWKVIWQNLYIFSNSDLLFIP
                     AFLNDFWEGHYNWREWNLPPSPYLFPDLFFSFLVSLLTQNLFISFLIYSISFSFLIVI
                     GLTMIIHPMDEYFSFQRILRVTTFFIAFWIILVSNYPEDLALFIFPSYHGSSFIFGLI
                     FYRIIFQIDNINKIIAFILLSGLVISSDTQIVYTFYIPFLLTSIFLKSALPEKLYIKV
                     FIYFVVSFVVGSFFLKLLGSINIFSVPSIPVWKEIKMNIFQLKILENIRNITPVVKVY
                     FMELYSDKILFFYLLIVSVPLNLYYAFKKTEVKTELRFFSLYVLLVLFVSIFTQMFFG
                     IWGGARYVWAIYFFPYVSILIYFGNSLSRMLTNGNYRKVEGMRKYVFLLSKDRIRSQL
                     ILFGIVIIFLVLFIFMYLIKGRVELTVSQPYPPFVSCLDELKKKYDLTYGISDYWNAK
                     HVSYLSKEKLFVNQVFVNLEKYNWINNRDWYKRDKENQSIHYHFILPERLDINRIKEK
                     FGSDYKTEICEGREVFVFEKGFYY"
     gene            complement(23113..23194)
                     /locus_tag="PLF66_06365"
     tRNA            complement(23113..23194)
                     /locus_tag="PLF66_06365"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(23158..23160),aa:Leu,seq:gag)
     gene            complement(23626..25635)
                     /locus_tag="PLF66_06370"
     CDS             complement(23626..25635)
                     /locus_tag="PLF66_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000877557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HMZ63702.1"
                     /translation="MHFKEIFEPIKINKTTLPNRIIMGSMHLGLEGLPETADRMIAFY
                     GKRFDGGVGMITTGGISVNHAGKGNIVFFNFQKEEDCKELEKVNKALSGKGVFCAQLF
                     HAGRYAYHRELVGPSAIRAPINRFVPHELTEEECWKTIEDFGQSALRAKQVGFGAVEV
                     MGSEGYLINQFFSPVTNQRKDIFGGSPENRMRFGVEALKKIRSMVGPDFPIVYRMSGI
                     DLIPGNPTFEEVVQMAKQLRDNGADALNIGIGWHESRIPTISMLVPRGAWAKIAQKIK
                     KEVTGVPIIASNRVNIPETILRILKEGEADIVSMARPMLADPDFVNKIKANQPERVNT
                     CIACNQACLDHTFKELMVSCLVNPEANREIEFSQIPKGKKSRVVVVGSGPGGMESARV
                     AASLGHDVTIFERSNKLGGQLNMAASIPGKFEFKETIRYFENELKALGVKINLNTECN
                     LQTLDELNPDAVIFATGVRPRNFNIPGLEKKKVGSYVDYLNGRFTLGEKVVIIGGGGI
                     ACDSAHKLLEDHDPTIDEYFAKYNVGSFTEVKIQPHTSKRKISILRRSGKVGSGLGTT
                     TGWALLQELQSSGVDFYTSLSYKEVREEGLVIDLKSSGEKVIECDSILICAGQEKEDS
                     LANSYKEKYPNKQIFIIGGAKETSGLDAKRAILEGSLAAREIGRS"
     gene            complement(25778..27313)
                     /locus_tag="PLF66_06375"
     CDS             complement(25778..27313)
                     /locus_tag="PLF66_06375"
                     /inference="COORDINATES: protein motif:HMM:TIGR00368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HMZ63703.1"
                     /translation="MFANMGYAYSSNLEGLNTKTIRVEVNLKRGIPRFTIVGLAATSI
                     KESTERVHVAIENSGFDFPLQSILVNLAPADARKDGSWYDLSIAFLILQLSGQIESKI
                     DIDKYLFLGELGLDGSIKPIRGLISIILNAKKENFKAVIVPHANRYEACIVEGIDIYT
                     ISHLSELNSIVTGTKEKEITGKRYDSILPFFPDLELYNDQSLALRAITIAVAGKHHLL
                     MVGHPGGGKTLLARLASDLQPPLSEKEYLDILRIKSNSETLLSEESLFVKRPFRSPHH
                     TSSDVSIVGGGRDSRMGEITLAHNGILFLDELGEFKPQVIQALREPLEERRITISRVN
                     YHITYPASFLLIAATNPCPCGYYGSIIKKCICPDFKADRYLSKLSGPFLDRIDINIRV
                     EAFKKENRRKSIVDLNSIYQFIQQAREMQRERYLNSEYNFNGDVDGRFLKTIFPIHPN
                     AEKYWEHLQNAPEISLRKINKIKKVARTIADLEGSYEIEEAHLYEALQLNQTVHKPFA
                     IAA"
     gene            complement(27862..29361)
                     /locus_tag="PLF66_06380"
     CDS             complement(27862..29361)
                     /locus_tag="PLF66_06380"
                     /inference="COORDINATES: protein motif:HMM:NF019040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HMZ63704.1"
                     /translation="MISRKQFLKYAALATAGLSLGDKLKLTAELEAASLSVPGRTLIH
                     VLLEGGPDFLHLIVPKPSSDTTSYGYKYWNNRTATISRGTSQANFNSWNNAYTKDYQE
                     FTLNGQTFGVLKGDSGSGVSYNGWLISQIQAGKVAILCNVKHSSSRDHSNSLMVAQTG
                     LYQSSPLQTSVQGWGGQVINVLDNSANGNARLLSMTTGLRQFCSTSSAALNKPVSFSD
                     SRSFGLTRTADLGTESNTDLRFKRARALQTYMQARYADATWNANFPLFKKFTAQHYKL
                     LTLTDSVKSVLSTSNPQSTDIANLYAKSGNTGLVLKNTTFGKQIANLYDALQVPALNM
                     RIASLAYGGWDSHKNQKTDIEPQFDDLFGTNKAFDVLFKQTGGSNVFANSVLMFYGEF
                     GRQLKANGDGGTDHGTGNFVLLVGGKVNGGVYGTLFPDKDKDNFDRSNRDINTTTAGV
                     FTSFDPIYDRIASWLNNGAMTGVSLSLNGETSIDTSGGFNLGTQTLINS"
     gene            complement(29361..31418)
                     /locus_tag="PLF66_06385"
     CDS             complement(29361..31418)
                     /locus_tag="PLF66_06385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63705.1"
                     /translation="MFNLKKSFQFLLTPIILLLISIFFLEDCNLVKCGNTSCQENNDF
                     VKAVLWRLATRPGRPGLYIEAVNLSGASDDPANYRGFAGAISKGSGSGQWNEQAVRDV
                     LRVFAYGGAASDADITAWANMEPSNAIVEMLGMWTVHSKLANASSDGNLSINPIEGSL
                     SRLSHIYGTNGPATSRSDFDQKSIPWNNSPARTFTQAVPTRGLNPFRQKIAFFETNYH
                     LALNQDKNVTDVQMFRHYDTVANDLARSFRDNLGYEDVLANMALSAPVATQYNHKKNV
                     CTGSVFSGNEDFAREYHQLYFGILGVGTDRSGLTTKDAIPSGNAESFNSHEQITIPQT
                     ARALSDIQVVQLGNDSFSDIATFGTTKHCSGNLSIYAQTNQGSTAEARIKSLSKLSIA
                     HPESKAALPLIIISNLADENLDSSNPLVGTSSDITTKIANIQALWNSMSKKNLIEFIR
                     KYAISSMFHNTTRVKYLSTFDRTLLNANLTVVSNNELLNGLVRYDWEIASENTNPFRP
                     DHDVFGGQTGLEASNTDDVFRNANNTSVSGQFGATGIWRNGSFVRIKDYRNFLPKQSD
                     ITADEIAKFLWRRIIGDATYSYFGALERAHIVSILSTGRDLNYMLCGNVNVSACSTKD
                     NITAESSITSGNNATLINNAASTTLFRTGSTTTALNDDNDRVGAAIDFILTNPFTFVQ
                     VGK"
     gene            complement(31590..32219)
                     /locus_tag="PLF66_06390"
     CDS             complement(31590..32219)
                     /locus_tag="PLF66_06390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63706.1"
                     /translation="MEFILYLLFFTLSFIVLSLLRHYFILFGDLERDQFDRSELIDFQ
                     KLIPRQMLSILEANGSMQDGLVAFILFSLISYGWSLLGGLIGSPHYTNEFGNYFFQSC
                     FLFIGFFFGYSPVVEIVLGSKKYSEPDSFLVRFLSQETCVLIGVGLACISSNLAVYGM
                     YHQMIFPFTILNILIISGLLIYKLKTTPTPRTEEKYGDEEEYNYEEESN"
     gene            complement(32219..33760)
                     /gene="purH"
                     /locus_tag="PLF66_06395"
     CDS             complement(32219..33760)
                     /gene="purH"
                     /locus_tag="PLF66_06395"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010574689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HMZ63707.1"
                     /translation="MIKIKRALISVSDKTGLIDFAKYLNNKGVEILSTGGTLAALKQN
                     GITAIAVEEYTGSPEILSGRVKTLHPKIHGGLLGNPDMPGHKEDLEKNNIPSIDLIIV
                     NLYPFVETIKKPNVTLEDAIENIDIGGPSMLRSGSKNYKHTVVVTEISDYTIVQEEME
                     KNQGSVSEETSFSLAIKAFSVTAMYDSTISNYFARLKGDRFPEKLTLGFKKKQNLRYG
                     ENPHQKAAFYEPVYAASEFSALQGKELSFNNMLDFDAAFHIAALLPKNTVSIIKHLNP
                     CGIAYADTTLESFKLAHRTDPVSAFGGVIGVSGEVNGDLAKKITENFVEGVIAERFTE
                     EAREVFSKKANIRLIEIESFKEALGEMDLRPVHHGLLLQDRDYDLVTVKDLKVVTKKQ
                     PTSEELQSLLFAWNVVKFIKSNAIVFTDKNSTLGIGAGQMSRVDSVELAATKAKKSGL
                     SLEGSFVGSDAFFPFRDGVDQIAKTGAKAIIQPGGSIRDEEVIKAADEHGLIMVFTGM
                     RHFRH"
     gene            complement(33762..34373)
                     /gene="purN"
                     /locus_tag="PLF66_06400"
     CDS             complement(33762..34373)
                     /gene="purN"
                     /locus_tag="PLF66_06400"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002975881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HMZ63708.1"
                     /translation="MQKKIVFLCSGKGSNFKAVVQHIQNKSLDVQIAGLISDKEGAGA
                     LTLAKEFGIPTHVIPFDSYRNEKDRYHELLEELLLKLSPDLIVTAGYMRIIKGSTISR
                     FENKMINIHPSLLPSFKGLKAIEQAMEYGVRFTGCTTHFVEEGIDSGPIILQSIVEIL
                     PNSTISDLTQNIHAEEHKILPLSVEYFIQDKIQIEGRKVFIRE"
     gene            34545..35696
                     /locus_tag="PLF66_06405"
     CDS             34545..35696
                     /locus_tag="PLF66_06405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63709.1"
                     /translation="MNIQKTLKFILALCLISLFHFSFFGFNFGKTNLIVHEWGTFTAT
                     HSANGKPQSGQRFAGRTLLPKEVYTLVDTTLNPKQKKSLRFRTESGYAVENGSEERAF
                     HGISILNDTVRMETPVLYFYTDKELELDVNVIFPQGSIGEWYPKRNSGEKISFDHVDK
                     KGFLVKSDETSYTSLDLKKYQGSISWKATILSPSENTPFTINTKSNEWISPRMTDSNL
                     VKIGNEIEKYIFYRGIASFEIPIQTQFKKIESENFLEFKNKYSEKIPFVFVLKNPTNN
                     PKTKTVLWKGNLNPNEKKELKLNDTKPIVFSEKDITEFKIALESEGLYPKEAEAMLNT
                     WKESYFETKGITIFWIVPESLIQKLLPIEFSIQPHEFKRVFIGRIKIEN"
     gene            35867..36382
                     /locus_tag="PLF66_06410"
     CDS             35867..36382
                     /locus_tag="PLF66_06410"
                     /inference="COORDINATES: protein motif:HMM:NF020302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADAR family protein"
                     /protein_id="HMZ63710.1"
                     /translation="MIQSNKPPEFYWDIQGEYIFFLKGHLSQWWHSSFRFAGKDFNCA
                     EQFMMYQKSVLMQDEETGKKILETDDPKEQQRLGRLIKNFDSNLWDKVKYQIVYLGNF
                     LKFYQNKDLREVLFQTDNKQLVEVNPKDIVWGIALSMENPDRLDPKKWKGENLLGSIL
                     TNVRRELQIGN"
     gene            36458..37618
                     /locus_tag="PLF66_06415"
     CDS             36458..37618
                     /locus_tag="PLF66_06415"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HMZ63711.1"
                     /translation="MANESTTQTGFELTSNSPDDPRMHKAWGYITALPSEYLIHFQGG
                     KIKEKSSGQGATCFKWPRDTVFIIPTSLKEILFDASQLTSDNVDIRIHCMVVYRIVDP
                     MRIYKLINFSNRQRGEEKLARMIADICRSQIKRFIAKLDLQSCYRKRKEEIADALGLE
                     LSNIVSHSETGWGLEVVTTEIQDIFIQDPEIFKSMQSLFKSSQLKSSRFAELETQKEI
                     EVREIEKESLLAEYRLKQQLEQQKNDAKIKESALLLDKQNEEKKFELEKLRAEKEEEI
                     RNYKLIQELELQRKEAEFELEKSKSQADAIRLTHEEELEYLQKKISVEANLSPQSLEK
                     EFLEKSLPVLAEAFAKNLNGMNYQVIKSDGANLDSPLLFFIQQIKEIFQQKK"
     gene            37950..38402
                     /gene="fliS"
                     /locus_tag="PLF66_06420"
     CDS             37950..38402
                     /gene="fliS"
                     /locus_tag="PLF66_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002723073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HMZ63712.1"
                     /translation="MSLSRKSAYSSGYDAYKSNEISTMSQGKLVVMLYEGAIRFLNTA
                     IENMTPRKYEIVNRNVIKAQDIVTELMTSLNMEEGGKIAEDLLSLYIYMKKRMLEGNI
                     QKDSKYLKEVVRMLTELKGSWEEIAKKENTVPATPVAARPAGGFSIKG"
     gene            38453..38716
                     /locus_tag="PLF66_06425"
     CDS             38453..38716
                     /locus_tag="PLF66_06425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63713.1"
                     /translation="MQTFLSYGDGESAVKITFQNERILDLLEHPKAKKDSEYDELNTL
                     VLDCISIQEKCDSMLVAISLEYKKELNTHMIKRQLKSHLQKNT"
     gene            38747..39217
                     /locus_tag="PLF66_06430"
     CDS             38747..39217
                     /locus_tag="PLF66_06430"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HMZ63714.1"
                     /translation="MSAMETDDAFQFFGLDRNATEKEMKHRFHELAQKYHPDKGEFNS
                     DVIFLELMKHKATLEKFFETRNEHIEAFKEANTDYSLYKEAKQMQNDSILKYFKTREK
                     FNNQTAPNADFEIQLKSDLAVAKTKFLQLLQKYPNSIWVADSKDSIHSIDVWLK"
     gene            complement(39427..40110)
                     /locus_tag="PLF66_06435"
     CDS             complement(39427..40110)
                     /locus_tag="PLF66_06435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63715.1"
                     /translation="MKLFILTPVQCNRKMKRLPPIGKIITDKRYDISKLSIVSSLFNY
                     RYAHIRQAQCKSVVKQFLRGIHVILLFGLISLFGCTNYSVSKSKTVPPILVSIANIVD
                     SNNNKITGAYRIKVRVTNQEPFLAGYKLYVGNTENAARNPADLNSGLSCTTGPSVIPN
                     QPLEYILDVATDVTQLPSASNRICTYQTSLTSGQYISFRTLLLSIQPSNQGGNRINPS
                     LPSNTLVVP"
     gene            complement(40215..40769)
                     /locus_tag="PLF66_06440"
     CDS             complement(40215..40769)
                     /locus_tag="PLF66_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010574695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HMZ63716.1"
                     /translation="MDILSQPVLILNTSYLPISIKSVRDAICMVLLEKAEVIKAAEDT
                     FIRSEKLKIPVPYIILLANYYQVPRKKTKVSRNNILERDNFTCVYCGKQPPHSKLTLD
                     HIVPRSRWESIPKDKKPSEFNSWENIVTACRECNTKKGNRLLSELRWKVPEIKKMDYK
                     LNHIPHINRVSAEKYGWNDYLIPV"
     gene            complement(40770..41375)
                     /locus_tag="PLF66_06445"
     CDS             complement(40770..41375)
                     /locus_tag="PLF66_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010419159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HMZ63717.1"
                     /translation="MDFLNLLVNFFSDYGYFAVFGVLILCGFGLPVPEDVSLVAGGVI
                     SALGNTNEHIMFGVGMAGVLIGDSIVFTAGLTFGEKIIKHPFVARFLTRERYETVQRQ
                     FEKYGKWVVFMARFMPGLRTPIFLTAGVTRRVSFLRFFITDFLAAIISVPVWVYLGYY
                     GASNFDQLMTWVHQGQITIFVILAVVIIGFILKKYLARRKL"
     gene            41428..42573
                     /locus_tag="PLF66_06450"
     CDS             41428..42573
                     /locus_tag="PLF66_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020771619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HMZ63718.1"
                     /translation="MIKTIDELYIAEDSRIADYGVKHYESRERVYEEKGHAFRLPLQR
                     DRDRIYHSRFFKRLQYKTQVFTNSEGDNFRTRLTHTLEVSGIARSIATNLGLNSLLAE
                     CIALAHDLGHAPFGHAGQDILSDLMKGNGGFEHNKQSLRIVQVLENRYPEFPGINLCK
                     VTLSGIMKHGGDYAKSELNSLRLQEGPSLESLITDISDEIAYTCHDIEDGLEMEYITM
                     ESLMYVSLWKEMYLISKEKYKDATKDLLARATIRGILNFLVTDLTTTTEENLKKHQIK
                     NFNDLKLAWRNKFEIADFSKETEPRLRELKKFLLDNLYHHEMVIQMSRRGQGIIEKLF
                     HYYIKHTEEIPENYRRRMEEDGKYRAVCDYISGMTDRYAQIQVDSIS"
     gene            42570..44450
                     /gene="topA"
                     /locus_tag="PLF66_06455"
     CDS             42570..44450
                     /gene="topA"
                     /locus_tag="PLF66_06455"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HMZ63719.1"
                     /translation="MNETLLIVESPSKARTIQKYLGSGYKVLASFGHVMDLPKEDLGV
                     EIENDFTPHFEITKKARKNLKEILAQAKKANKILLAGDPDREGEAICYHLSLLLKQKP
                     NLKNRVELPEITKKVVLYAIENPVPIHKNRVESQYARRILDRIVGYKVSPILWKCLGK
                     STLSAGRVQSVVLRWICEREKEIDLFEKKEYWKVKAFVETKKGESFTLTLEDKEKKIN
                     SIESLKQTLESLLNQKNSEVKEGILDLKSLKKEMEVIEIKKEAKKEYPPPPFITASLQ
                     KEAGRKLKFSTKKTMQIAQKLYEGVFAGSDNGFMGLISYMRTDSTRINSDFKTKAKNF
                     IEKSFGKEFVGNDFIRKQKKEIQDAHEAIRPTDISITPESLKGIINKDDWELYQLIWK
                     RTIASLMIPAEILEESMKAKLNGFVFTGSGKKILTKGYLVLSTDSKVETYLPELKVGD
                     YLDLIQIESEQKFTEPPPRYTEPTLVEKMEKEGVGRPSTYSNTIEVLYKRKYTTQKQK
                     SIYPTELGMEVNEFLTKELAYLFTDHFTKDIEDELDKIAEGSLNRTDFLKKFYQEFSI
                     KTGQISKQKFVPLDKKNQPKQQCPLCQSKVSLRKNPKGREYWICSRFPECEYMAYKET
                     VK"
     gene            44478..45419
                     /locus_tag="PLF66_06460"
     CDS             44478..45419
                     /locus_tag="PLF66_06460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63720.1"
                     /translation="MRHTLVILILFLTFGINLFADSKQEDKVELIISDPKKAEGSWGK
                     EADEVEAFQDVKIFDDLSEENTKHRLEEARDFFNSSLAVYKATEKAIKEKKELHIKEK
                     ESNQQDKFEWQKKARESTLDKEYKRMSLEGRKKAVIELVKAMNALDKIENPDAITSPV
                     FIDLKASIYREYIKHQFRLKNYNQSSEVLEMYIALGEQYEKEAEPHKLLAMCYESQEK
                     QTSRYKKDSVSNEYKMLKNCHLLRFADLAYGVESNQFKRIEKKTVKFNPTVPNAMTGK
                     DYAIACGFLKKDGNKESKEDKKEVLLKKDEIKDTSKK"
     gene            45705..47957
                     /locus_tag="PLF66_06465"
     CDS             45705..47957
                     /locus_tag="PLF66_06465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HMZ63721.1"
                     /translation="MKNRLFFVLLLILLIVGCLKTTKSLFDLSSPSASNLSLIYFALT
                     NCCRVSGNITGFTSGTMDITLNYAPTSQNAQAQVTIKLNAPTSNYFFSEKIPSGYYYN
                     VFVSQHPSGLSCSVSNNTATITDSLTNLDINCVSAAITSFYSGYPDWMDYVKRDTPTS
                     NSLSLTGTACTGTELGWYNSCVHVGEIRSITIPNFTSCTDITATDSLGVFNWTCKAAN
                     NTVQVVSTGLKFDKGLTDLIDFTNVKWKNIYIVVNYKGSMLLTSVSNTWWTNPISVAN
                     SGLAAGVSKTIYIVTSNSTSQYTISQNRVAIVVRSGSSVVSGGGDALTISSRFNWIEG
                     PFIGSGSNGLNFAGTPSFNVIRNSVFSGLFAISNNNSKYNYFHNISIYGSNLGANMAN
                     PANIINGLNVYNFLPGGSFSIGSSDMIFLNSIISGNSSTSVLLGGSNNSNILYNTTLA
                     NSGNTGINLSAALRTRIVNLATISNNGDGITTNGSNNTEFVNTVAASNGTEISDFSST
                     MTFNGIFKTAVASCGISGGSPGITGTCAKAGSSETSPATVTNANISNSFVGKVTSDSS
                     NTIGASGTANLGSISGKDWYSFQNLYRSWGIGNVASFPSSVLRGNCSSTCQIWDWSLK
                     NTDSVLRNVNSCPSGNNVLTHAWNTGYGSQSLCDTNYPSSIFSAGVCSTVFLRNAVEI
                     FGDGIGNDNGLCESNEDCFYTPNIGAYQGHGTLVSASLTTFNTNNCSDIGVGGTISNV
                     KLWKWDTNGY"
     gene            48078..48527
                     /locus_tag="PLF66_06470"
     CDS             48078..48527
                     /locus_tag="PLF66_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010574447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63722.1"
                     /translation="MIKIADTRWLPEHKLIITELRGNVNSEDVEKWISSLYQALDQIE
                     DNGVFKIFINLMDFQAVDLDVHKKYREIIPRTLGEYGWKVGYVDMFERAKELQLSNKR
                     GIQCVAAVHVHQDETKIQKYEDNYSRENEHYFTDIKKAEDWIYSYPV"
     gene            48754..49236
                     /locus_tag="PLF66_06475"
     CDS             48754..49236
                     /locus_tag="PLF66_06475"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HMZ63723.1"
                     /translation="MKNFLLIPFLFLLIHCQKDFNAPYLSTELKDLEGKKHKIIDYKG
                     KVLILDFWASWCEPCKKAVPVIELLRENASSEDFVFIAVNTDNDLTEIEIRKVANQFG
                     IKYQILLDPDLELARQLNVEGLPGLFVFDKAGQNIYHQYGVNPEDAHDLLKKMSEWQK
                     "
     gene            49330..50685
                     /locus_tag="PLF66_06480"
     CDS             49330..50685
                     /locus_tag="PLF66_06480"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002774113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HMZ63724.1"
                     /translation="MKFTTFDNQRIVLASGIRTPIGQAGKSLADIPSAELAYRVTEEI
                     FARSKVSKTEVDGVVAGEISQSVYTPNVARVISVRAGIPLEASAITVANNCVSGSEAI
                     LDAARRIMIGEGKFYLALGLESMSQGPFIVEGAKSVTKLDTVDKLMKNWGEAQALGVK
                     VIDSIDEGLNDPIRKINMAMTGEVVAQRYGLKKAELDDYAYRSYKKAYDSIQAGNYRP
                     YQMPVKLTDGTLLEDDEYIMSKTGMVENPGRFAKAGAIFDSKFMSIKEFYEKYGEWIG
                     RPYVEGETEAAVTLFNACPRSDGAAAIFVTTEKEAARLGLDVQAVITGWGMYGVDPSI
                     MGVGMAWSMNKAIKAAGLDFKDIDNFEIHEAFAATAMGTMREIKKSWGFDLEAVDKEG
                     GRVNPNGGTLALGHPLGATGLRVLLNQIMFFKQNPSAKRSVNCICAGGGVAGSILLER
                     P"
     gene            50846..52468
                     /locus_tag="PLF66_06485"
     CDS             50846..52468
                     /locus_tag="PLF66_06485"
                     /inference="COORDINATES: protein motif:HMM:NF012368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HMZ63725.1"
                     /translation="MTEIHFLEDKIVSEENLNLSLLEISLKNEIPHIHACGGNARCST
                     CRVIILENLENVEPRNEKEKKLAEIKGFEKNIRLACQTHITGNVKLRRLVIDKEDIEI
                     AISEKSETTGKEKKIAILFSDIRGFTAFSEKALPYDAIHILNRYFNRMGAAVLENDGY
                     IDKYIGDGLMALFGLKEEDPDMICLKAIKAALSMIEELNDLNRYLKDHFDLEFKIGIG
                     IHFGNAILGEIGHPGKRQFTAIGDSVNLASRIESTTKKANAEFLVSSDVYSILKTCIN
                     IGRVFETKLKGKTGVYRLYEILSVKEHRLSPIEALQSSLYKFMTLEDAPKFLRLVFHD
                     VSNFNVETKTGGANASIRFELEREENQDLRTVFTKVEIIKKDLEEKGFNISYADIIAI
                     GGAVAVYKTGGPRIQMSLGRIDATEAGNQIHITTPDIDIKELLYRFSLLGLEPKELVV
                     LSGAHTIGKANGKPMTDDLFRFTNSYFKKLLEFCHGARNENLAILNSDIVLLDMQETR
                     KWVELYARNEIQFFEDFSKAYQKITSFGKMGV"
     gene            52627..54549
                     /locus_tag="PLF66_06490"
     CDS             52627..54549
                     /locus_tag="PLF66_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016549372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HMZ63726.1"
                     /translation="MANNLAELYRESAEKYGSMPSFFSKDEKKQYQPVNYKDLYEMGL
                     NLGEALIELGVQARDKVGFIADHRLEWTVTDAGILLTGAANVPRGTDVTEAELEYILN
                     HSEAKVLFVENDKVLEKFKKVKSKTSVKTVIMMDPKAKGESGVLKLYDLIEKGKQLRS
                     GGTKKVEARVADIKPDDLFTLIYTSGTTGQPKGVMLMHSNMMHQVNDVVPTLEIYSSD
                     RVLSILPVWHIFERVFMYAAISAGASTYFTNVRDLREDFTKAKPTFMGSAPRLWESIY
                     LGIYNRLNDPKQTPPARKAIFNLAYFFSKHFHASVRFIQGNEVDYEGRNPIVSLLTGI
                     KSLIVAILTFIPYVLLDAVVLSKIRMATGGSLRATCSGGGALQRHVDAFFNDIGVKVI
                     EGYGMTETSPVISARSFKHLVMGSVGKVAPKTELQIRDFNGAVLTHVKLDGSIEGKRG
                     AKGIVHVRGPQVMKGYYKNPEATAKALKDGWMDTGDLGMINYKDTLTLTGRAKDTVVL
                     LGGENVEPVPIENKLTESVYINQVMVVGQDQKNLGAIIVPDFEKLKAWAKENGISESD
                     EKALIENPKVVELYKKEIKELNSAKNGFKSFEQVTPFFLITKPFEVGDEMTNLMKLKR
                     HVITEKYKDQIKKIYM"
     gene            54764..>55150
                     /locus_tag="PLF66_06495"
     CDS             54764..>55150
                     /locus_tag="PLF66_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010569102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HMZ63727.1"
                     /translation="MKLNPEQEAAVRHFEGPLLIFAGAGSGKTRVITNRIVYLIQKKK
                     VDPKQIVALSFTNKSSKEMKDRVRKLLNKKESRGLTLSTFHALGLNILKKFIDKLGYH
                     NPFILQTPGDLEGIIQDLLKQRKIDTK"
BASE COUNT        19109 a         8726 c        10053 g        17262 t
ORIGIN      
        1 gcatccaaga agccattcga ctcatattga atataacgtc ttttacagga tattgatctg
       61 gtaatttttt tctagaatca tttgtagaaa cctcgatttg aggaataaaa agaaatagtg
      121 atattgaact aggaaatttt tctttaaaag aaattagctt tccctcatga aaataggaaa
      181 aaacaaaacc accaatcatc gcaggagttg tattgtcagg gtgtccctct aagagagcta
      241 attcataaag aattttatct agattaggaa ctggaaattt tttagaaaaa tgttttttgt
      301 gtaaataatg acctaatgta aaacctgcag tgagtgcact cgcacttgat ccaagtccac
      361 ctttgaaagg taaatttaaa tccattttaa cgttatacgc tattaacttt tgtttatgca
      421 agaaaagatc aaaatatttt ttataaccct ctaataataa atcttcttta tctgaaaaag
      481 ggaggggtgt attgtcgatt ctcagtgtag taaaatttga attgggatct atggtaaaat
      541 aaaactcatt gtaaatttta agtgccattc cgagtaaatc aaatccaggt cctaaattgg
      601 cagatgttcc aggaactcgt atatatattt tatgattcat gttgttttta tccagtcttg
      661 tatcttatcg atacagagat tgtagacaga ttctagttta tctttttcct cagaactaaa
      721 gcggcttaat acatagtctg ctacttctct tccatcttta ggttttccaa ctccgactct
      781 caatcgatga aaatcagcag atcctatttt cgcaatgata tctcttaaac cgttatgtcc
      841 tgcatgaccg cctccaattt tcattttaca ttttgcaaat tcaaaatcaa tttcatcatg
      901 cacaactagt aattcagaag gattaatgcc gttttttcgc ataaataaag aaacaccatt
      961 gcctgaaaga ttcataaatt caaacggttt aatcagtata atttttttat cgaataaaaa
     1021 caaatccgtg ctggggcatt ttttgtctga attaaatgaa attccatgtt tatcagatag
     1081 cttatctagc acagcaaagc caatactatg gcgcgtgccg ttgtatttgt ctcccggatt
     1141 tccaagtccg gctattaata atttgccgct cattttagta ttagatttaa cattttctcg
     1201 gttgcaagga tagcagcaga gatttgatct gaatctaaac caatggttct aaacgaattg
     1261 gtctgtgagt tatgaacgtt gcctgaccat gtaataattg tttccacaag tgcagatgtt
     1321 ttaccgccgg gaggaattct aacttggtaa tctaataatt ctggaagtgt aaaattattt
     1381 tccttagcaa tttttctaag ggcatccata aaagcatcat aacctccatc tccttttcct
     1441 tctgcgtagg attctttagc tttatacttt atggaaagct ttgctgttgg ggaaatgccc
     1501 ttaccagata aaacctcgca acgaagaatt tggaaattgg tatttaaatc ttctccggaa
     1561 agatttgcaa tcagaaacgg taggtcttct gtagatacaa taattccttg atcgcccatc
     1621 tcaatgactt tatctaatat tttcttttcg aggtcacttg atagaactag tcctaattgt
     1681 tttaagtttt ctgagatact tgcttttcct gcaagttttc caagtgcata gcttctcttt
     1741 cttccaaagc gttctggcaa aatagcattt gcgtataaat ttcctttctt gtctccatcc
     1801 gcatgcactc ccgcagtttg tgtaaaaaca tcttgtccta cgattggtct attatcagaa
     1861 attctttttc cgctaaatac ttcaactaag cggctaagcg ctgttatctc tgattcgaca
     1921 atattagttc taaacgctgt tttatcatga attgcagtaa tcactgcttc gagtggtgaa
     1981 tttcctgctc tttctcctag gccgttaact gatacatgta gaccggaaac acctgcattc
     2041 actgcagcca aacagttggc tactgctaga tcgtagtcat tgtgcccatg aaattcaatt
     2101 ggtttgtttt ttacaatttc tctcatgaag cgaattgctt ttgttgtttc gtccggagat
     2161 agaacaccca atgtatctgg taaaaataat cttttaatgg gaaaatctga aattgctttt
     2221 gccattttgg caacatagtc tggtgaattt aaatatccat ttgaccagtc ttccatataa
     2281 acattaacag tgattccttt cgagagagca tagtcgatag tcgccttaat atcatctaag
     2341 tgagcttctg ccgttttttt taattggtgt attaggtgtt tttcagaacc ttttgttaat
     2401 agatttaaag tttttgcacc agattctaag atccaatcaa cacttgcatt tttatctaca
     2461 aagccaagga tttcaatctg gttggtcata ttttcctttt ctgcccaatt cataatatgt
     2521 ctaacggtct ctttttcacc tctagagaca cgagcagaag caacttctac tctatctacc
     2581 tttgtgttta gaagcaaaaa tttagctata ttcagttttt cttggctgga aaaaacaaca
     2641 ccctgtgttt gttccccatc tcttaatgtt acatctaata tttctaattt tgtattactc
     2701 atctgatata tttttttaaa tcctgtttat cgagacccat gatttcaatt tttgttacac
     2761 ctggatttgg tgttatgaac tgctccgtat ttacaagtcg aagataatca taattacgaa
     2821 ttgtctctaa ggtcagttct tttagttttc cttctcctat tccatgcaca acttgaacga
     2881 gtgtttctcc tcgcataaaa gcatcttgaa gctcgcgttc caatttttgt tttgcctctt
     2941 catagcgcat ttttcgaatg taaatctcga tcatttcttt aaaaccagtg tttaatatca
     3001 ataatttaca atgtatgcta ggaaaaaaga agtttttatc gataggattt ttagattcaa
     3061 cgaatttgaa gggaatatat ttataagcta caagctaaaa agattttcta tcccatctag
     3121 gctctagtag tttagaacct agtaaatctt aagtgaacat gaattcgaaa taagaagatg
     3181 tttcattttg taacccccga attccacccg atgccattcg catccaatac cgccggggag
     3241 attatcaggg gtctttagag ctacagattc ccgatatcgc ttggcagttg cggcgatagc
     3301 tggtggaatt tcaggaatga cggtctgaac aatttatcaa atccttctgc cgaacacacg
     3361 ttaagttaga attgagaggt tataaccaaa atgaaagggc aggaaaaaaa tttctcccaa
     3421 agactttgtt tcaatagtat ttgaaatacc gatttttgaa aaaaagtttg ataaaactta
     3481 gaaagattta aaccaaccgg aattttttaa ttttgaaaat gggtctttct tttcatgtct
     3541 ggaatcagtg gatatttttt aaaaatagaa cggaaaatct gaattttgac ttggtattgg
     3601 attgaatctt ttaaaataaa atttaggtta tccctataga aattgtcttt ttagagcatt
     3661 ttttttctat gataagaagg aaggggagta gatttatgaa tgaaaattcg attttagaat
     3721 atttccaagc tgaaaaaatg gaaagtttat tgtttatgat tcttggagga atagggatat
     3781 tagctgcaat ctttgttttt atttattcga ttcaatataa aaatataatc tatccattca
     3841 tagtgattgg gctgatccaa atcattgtgg gtggatctgt tttttttaga acagataaac
     3901 aggttgagtc tctgttaatc cagttaagcc aaaataaaat cgagtttaaa aagtccgaaa
     3961 caaacagaat gcaaatcgta attaaaaact ttgaatacta taaggctcta gagatttttt
     4021 tgattttttt aggtatagta ttgtcttatc tttatccagc aagagtcggg atttattcca
     4081 ttgcaattgg tttgatcttg gaatcatctc taatgttagt tctggattta ttcgccgaaa
     4141 agagagcaat agactatctt caatggattc aaaatttaaa ttaggtttga ttcagtttta
     4201 aaaatgaatt tagaaagaaa atatttagct tttaactcga ttgtaagttc gacacaaaat
     4261 atactttata tttacaatgc cgaaaagaaa gagattgaat ttttaaatcc aatggcagca
     4321 gtccaagtag atattataaa gaaagaagag agtaaaatat cgatagagaa ctttatagaa
     4381 aaaatccatc cacaagacag acttaaatat ttaagtcaaa ttgatggagt tctctctggg
     4441 cgcgaaaaga aagaaaaacc aatcgaatac aggttactaa gaaaaaatgg agatgttttt
     4501 tggattagcg acactttaat tctattaaag atcgaggatg ctgaatctaa agagatagtt
     4561 ggatttgcgt ctaatattac aaacgttaga cgtaaagaag aaaaatatca gaatgaagaa
     4621 attcgtttaa gagccattct tgaaaatgca agagagcaat attattttat aggggtggat
     4681 tataaagttc aatctttaaa taaaaatgca aaggactttt taattaaatt atataatcga
     4741 aatatatttg agggttattc aattttagaa tttctcccat tggagaatcc gaatactttt
     4801 acagaaaaat ttaattatgc tttaaatgga actgcaattc aatatgaaac acaaatcaaa
     4861 gatgagaaag gagaaacaaa ctggtttcaa ttcagctatg ttcctgtccg aaataaaaca
     4921 aatcaaatca caaatatttg ctttatcatc gtagatatta ccgagatcag aaaagcgaca
     4981 aagaatctaa tggatttaaa tcaagaattg gaggatagag taaaagaaag aacccgagaa
     5041 cttttggagg aagtagctca aagaagaatc acagaaagcc tactgcaaaa atcactgata
     5101 aaagaaaaag aactgaacga attgaaatca aaatttatcg caatggtatc tcatcaattt
     5161 aaaacaccga tgacaacgat ttcaatgtct acacaaatgt tagaaaatta ttttgaaaaa
     5221 caaagtgaag taaagagaag aagacattat agtaaaatta aagaagcaac ccaatcctta
     5281 aaccgattga tcgaaggaat tctaagtgta agtcgttccg aatcctcatc tatgcccttt
     5341 gatccgagta aggttgatgt cccaactttt ttacatgagc tgataggaaa ctttcaatcc
     5401 aatcatcctg attatagatt tgaaatccga ttcgaaaaaa agaataaaac agagtataat
     5461 atagattcaa atattatttc tcatattctt gaaaatttat taacaaatgc agtcaaatac
     5521 tcccccgtga atactacaat ctatatcact gcagtcttag aggagaaaag cctacttttt
     5581 attgtaaaag acaatggtct aggtattcca aaagaagatc aagatagaat ctttgaaaca
     5641 ttttatcgag gcaaaaatgt agcaaatata tcaggaacag gacttggtct aaacattgtt
     5701 aagaacttag tagaattaca tttaggaaaa ataaatttta agagcagtga atcagaggga
     5761 actgaatttt taatttggat tcctgatata gtggagaatg tagatggaaa caattttaat
     5821 cattgaagat gatgacttga ttcgtgaaaa tatggtagaa ttatttataa acgaaggcta
     5881 tcataccata gctgcgtcta acggtaggct aggattagaa gaagcaaaaa ataaaatact
     5941 agacttaatt atttctgata tcatgatgcc agaaatggat ggaatagaag tttataaaaa
     6001 tatccgatca gagcctatct tatctgcgat tccattttta tttgtctcag ccttatccga
     6061 aataagcaat gttcggtctg gaatggtttt aggagttgat gattatataa caaaaccatt
     6121 tttaaattca gagctaataa ccgttgttaa aaatagaatc gaaaaaagta gaaatcttag
     6181 gaagtcatta gaagatttaa aatcaaatct aattcggtct gttccgcatg aatttttaac
     6241 tccactcaat gcagttatcg gtttttcgca attactaatg gatggttgcg gggaattaca
     6301 caaagatgaa atatatgaat acagtcatta tattttttct gcgggacaaa gactattacg
     6361 cataacgcgc aactatatat tgtttactga tttaacaatg aatgaaaaag aatataaaac
     6421 aagagcaagt ctagaaaatg aaatctatga gaatattgaa tattttttat ctgaatattt
     6481 gtattaaatc gcaaacaatg aaaacagaag acaagaccta aatcttaagt tagaaccagc
     6541 taatttaaga atttcagata aaaatcttag aaagattttg gaagaacttt tggacaatgc
     6601 aatcaaattc tctaaaaaga atataaatat aacaggacaa tatgtaaatg gggaatatga
     6661 gataatgatc caagatgatg gggatgggat ttccatgacg aatctacaaa atattgaagc
     6721 ttttatgcaa tttgatagaa aaatgttgga acaacaagga ttaggtttag gattggctat
     6781 tgtaaagaaa ttatcttcta tttcaaaatg tagatttcat attgaaagtg tagtaggtca
     6841 gggaactaaa gcatttttat atattccgct agccaaaaat gaacagggtt agacatgatc
     6901 taatgtaaag atctataaaa aatttcattg actttcttag taatcatagg atactaccta
     6961 agttgatcga tagtttctct tatgcaaaaa cgtctaagcc aaaagtccta atatgaaatg
     7021 aatcactgat tacagctaag tttggacaga tgaacgataa aattaaaatt ccaagatatc
     7081 cggttctcat tgtagaagat aaaaaagaga atcaagtatt aattcaaagt atttgtaatc
     7141 agattgggat acaaactgag attgctggaa atggacatat tgcactcaat ttagttaagg
     7201 agaataaatt ctccattttt attgtagatt taatgctacc agtcctagat ggtaaatctt
     7261 ttatcgaaga gctaaaaata attgagccga atgcagttgt cattattcaa tcagctcttg
     7321 attcttccga aacaatcatt gaggttatga ggcttggtgt atttgattat gtaattaaac
     7381 caattgatat agataacttc cgtcatacat tatcaaaagc cctcgaattt aaacatttaa
     7441 aagatctaga agccgaactc atcttaaatg agagtataaa attacgcggt caattagaat
     7501 ggctcaatta taaagaaaca ttgcgactaa cgggtaaaga ttcttatcag aagaattcta
     7561 tttttaattt aacaacatct ctatctcaag gtggaggagt tggagtcaca atatcccttc
     7621 ttgatatgct aaaaatggat atggaagagc aaggagatag atacattgtt aataaagaaa
     7681 ttctagatac actatttgaa aacaatgaat acagcagaag agtcattcaa ggtttaggtt
     7741 ctgttgttga tttaatggag caaccctttc agtttagcct ttcttccgcg agtagtttca
     7801 taaaagaatt tcccagtttt tttaagcata taattccata tatgcaaaat aaaaatataa
     7861 cggttgctta tccgtccgtt caatcaacag tgcaaatcga aattgatata gaaaaaatta
     7921 aactagtttt agaagaactc ttgattaacg cgtataaata ctctgtcagc tcaagtagga
     7981 tagatatttt tgctcatata acaggaacat atttttgtat tacattcaaa aatgaaattg
     8041 acgaaaattc ttatggtgga attccacctg agaaggaaaa actagtatta gagccatttt
     8101 ttagaattca tccgccagta gaagatatac tagcagttga aaaatttagt ctaggactcg
     8161 gacttactgt agtagatttt ataatcaata aacataacgg tatgtttttt atacacaatg
     8221 ctgttgacca tactggaaaa aaaagaacaa actgcgtact tgcagaagta tttttaccta
     8281 ttaaattaga aaggagttaa gcttgtccaa gagaatttta ataatagatg actctgctgt
     8341 gtttcgaaaa gttgtttcga tgaatttaaa gcatgcaggg tatgaagtaa tagaagcagt
     8401 agatggattg gatgctttta ataaattacc gacgattaaa ccaaacctaa tcgtatgcga
     8461 tgtcaatatg ccaaatatgg acgggctaac ctttgtgacg aaagtgagag cggatgaaaa
     8521 taatcgattt attccaatca ttatgcttac aactgaatcc caagaagaga aaaagaaaaa
     8581 aggaatggaa gttggagcga aagcatggtt agttaaacca tttgcaccag aacaactttt
     8641 atccgctata tctaaattaa ttgcataaat atgagtgtag aaattgaaat taccaatcat
     8701 agtaaccatt gtaaagttac gttagttggt aaatgcacga tatacaatac aataaaatta
     8761 aaggatacac ttctatcagt aatccaatca aatgaaaata ttctaataga tcttgcgaaa
     8821 gtaaatgatt ttgatacagc gggaatgcag ttgtttatct ctgctaagaa cacagcaaaa
     8881 aaatacaata agaagatcaa atttgtttct catcctgatt gtgtgttagc tatcatggat
     8941 ttgtacggct tagtcggttt tttcggggat aaaattgttt taagtcaaga tggaagaaaa
     9001 aagtttccat ttaaatacgg gacaaagaaa aggaacgcat agcctatgaa tgcaaatctt
     9061 gatgaagtta aggaattatt catttctgag tctagtgaga ttcttcaaaa aatggagagt
     9121 attcttcttt ttttggaaat gaatcctgtt acagatgata agattcatga attatttaga
     9181 tcgattcata cgattaaagg ctctgcaggt attttcggat atgaagagac cgttagcttt
     9241 acccatgttg ttgaaaactt attggataaa gtaagagaaa aagaaataca actcgaaaaa
     9301 cctcttatct cccttttact tgattgccga gatcatattt cagatttaat tgagtatgcg
     9361 gctaacggtc gatcattaga agagcaaatg cggtcagttc aaaatgatct actggtaaaa
     9421 ttaaactctt atttatctaa ttttaaaact actacaaaag agaaagatac tcctgcaaag
     9481 gaaagtgtca ctccaatacc tctttccaat ccgaatgcaa attggcatat ctctcttcgg
     9541 tttaaacaaa acacatttcg agatggttta gagccatttt cagttttaaa atatttaaac
     9601 aaagatggtg agattttaaa cctagttaca ctaacaaacc tgattccaga tttggaaaat
     9661 ctacaagcag aagattgtta tacaggcttt gagatagaat ataaatctga taaagacatg
     9721 agttttattc tgcaagcatt tgaatttgtt gagtatgact gtgcaattag tatcttgcct
     9781 cctgatagaa cttgccaaga tatttataat tttttaattg atccgaatta taagaccaaa
     9841 aggctattaa gaattttatt ggaaatgggt gcaatgacta gagatgagtt gcaccaaatg
     9901 aatcaatgga aacgtaataa aacatctaat acaactctga acgaatcctt accaactgtt
     9961 gaggatgaga gtcgagaaat acaacaaatt gaaaatatag aaacttatgt tgagaataaa
    10021 caagttaagc agaccaaaga aagcaatcga gaaaataaaa tcattcggat tgattccagt
    10081 aagttagatc atttaattaa tttagtaggc gagcttgtta tcggtggtgc aaatattaat
    10141 cagctttctc aaaaaagagg agacccagaa ttgtctgaat ctgcttattt catgaatcac
    10201 ttaattgggg aaatcagaga gactgcatta aaaataagaa tggttcagat tggggaaact
    10261 tttaatcgtt ttcagagaac cgttcgagag attgggcatg agttaggaaa ggaaattcaa
    10321 ttaactattt caggtgggga aaccgaatta gacaaaacag tcgtcgaaaa aatcaatgat
    10381 ccattggtgc acctaattcg aaatgcaatc gatcatggaa ttgaacccaa ggaagaaagg
    10441 gtagcaaaag gaaaaccagc tgttggacaa ttaaccttaa atgcatttca tgaaacggga
    10501 agtattgtta ttgaagtaaa agatgatgga aaaggtttaa accgagaaaa aatcttaaac
    10561 aaagcacaag agaaaggact tgtttctggg gataagcaac tttctgattc agaaattttt
    10621 aggctcatct ttaaagcagg tttttcgaca gcggataaaa taaccaatat atcaggtcgg
    10681 ggagttggct tggatgttgt cgaaaaaaat atagatgcgt taagaggaag tgttcaagtc
    10741 ttttcagaac aagacaaagg aactacattt aaggttagat ttccactaac gcttgctatc
    10801 attgacggat tcctattcag agttggaaaa tctttctatg tagttccatt ggatttagta
    10861 gtggaatgcc ttgaatataa agaagaggaa tctgttgatg gtagcaagga ttatataaac
    10921 ttacgtggtg aagttttacc atttattcga ttaagtgaat tctttcagac cgaagaggaa
    10981 tcaaatacgc gaaaaaatat cttagtgatt caatatgcgg gaaaacatgc tggcttattg
    11041 atcggtactt tacatgggga aatccaaaca gtcattaaac cacttggcaa agtttttgag
    11101 aaattaaaag gtattagtgg atcaaccatt ttaggaagcg gtgaagttgg cttgatcata
    11161 gatataccgt ctctttttaa aaaggtagaa gaaatagagc gaaaatttgt ttccggtcga
    11221 aattaaaatg ttttaataat aatttttggg gagaattcaa tgttgattag aaatatatcc
    11281 attcaggcga aattagtttt actatatttc ttggctttct tttgttccgg tcttctaatt
    11341 tctttttttg gaattggcat ttctgatttt gaaagtaaga taaatacttt tataaacaat
    11401 caaaataaaa caaatattga atttaaggat ctgcaaatag aagtaaatga atcaaccttt
    11461 ctattaagcg aaatgattct ttctcagagc ccagaaaaag caaaatcaat tgcacaaaat
    11521 ttagaattaa aaaatgattt ggtgattacc aaattagaaa cacttcaaaa acaggttcag
    11581 gatccttcta taaaagagga attgttaaat ttaaaaaata attttgattt ataccacaaa
    11641 gctgcaaatg aagtgaagag cttagcaatg gaatctaagt ttatcgaagc tgaaagtctt
    11701 taccaaaaga atataaatag ccttttgata aaggtgaaga caaatgaaaa agaattaaat
    11761 caattatttg aaaaatcatt caaagaaaat tcagaaaata cgatgactgg attcttgcat
    11821 caaaaatcag accttacaac caaatcagtt ctatttgttt ctgtaatcct gcttttaatg
    11881 ggttttatct tattcggtgt tgtttttact attaaatctg ctcacaatag ttttaaaaga
    11941 attcattcag gagatttcaa agaacaatta aatgaaattg gaaaggatga aatcgcccag
    12001 atatctaaaa catttaatac tttttattca agctatagtg caatcaatcg atctcaggct
    12061 gtaatcgaat tttcaattga tgggacagtc ctttcggcta acgataattt tttaactgct
    12121 atgggataca ctttaaatga aattaaagga caacatcata ggatgtttgt ggattcagct
    12181 ttttccctat cggaaggtta tagtcaattt tgggcatcat tagccagagg agaatttcaa
    12241 tctggagaat ttaaaagaat cggaaaaggt ggtaaggaag tatggctaca agcatcttat
    12301 aatccgattt ttgattcgaa aggcagacct tttaaggtcg taaagtttgc atctgttgtc
    12361 actgagcaaa agagaaaaaa tctagagtat gaaggacaga ttgcggctat cgctaaatca
    12421 caagctgtta tcgagtttac tatggatgga ctgattgttt ctgccaatga taatttttta
    12481 aactcaatgg gatatacgct aagtgaaatt aaaggacaac atcatagaat gtttgtggat
    12541 aaaaactttt ctttgtcaga ggattatcga cagttttggc tggcattagg gaaaggggag
    12601 tatcaatcag gagaatttaa acggattgga aaaggtggta aagaggtatg gctacaagca
    12661 tcttatacgc cgatattaga tctgagcggg aaaccaatta aggtaatcaa gtttgcatct
    12721 ctaatcacag aacaaaaaca aaagaattta gaatatgaag gacagattgc ggctatcggt
    12781 aaatcacaag cggttattga atttactatg gatgggctga ttgtttccgc caatgataat
    12841 ttcttaaact caatgggata tacgctaagc gaaatcaaag gacagcatca tagaatgttt
    12901 gtggattcag cgtatgcaaa tagtgaagag tatagacagt tttggaattc actcactaga
    12961 ggtgaatacc aaaatggaga atttaaacga attggaaaaa atggtaaaga agtttggttg
    13021 caagccacct ataatccaat tttagattta agtggaagac cttttaaagt tgtaaaattt
    13081 gctttagagg tgactaaaca gaaattaaaa aatgttgaat atgaaggaca aatcagagct
    13141 atcgataagt ctcaagcact catcgaattc agaatggatg gaacaatttt aactgctaat
    13201 gataattttt tagcagcaat gggatataac ttaaatgaga ttaaagacca gcatcataaa
    13261 ttatttgtag attcttctta tgcagcatcg gaggattata gagaattctg ggcttcttta
    13321 aatagaggtg aatttagggc agctgagtat aaacgtttag caaagggtgg aagagaggtt
    13381 tggttgcaag caacttatag cccaattcta gatctaaatg gaaagcctta caaagtgatt
    13441 aagtttgcaa ctgttattac agagcaaaaa atattagcaa atgagacagc gcgtatcgtt
    13501 tcggatttaa ttccatctct tgctgcactt gaaaaaggag atctaactaa cttaattcaa
    13561 agtgaatacg aaggaagctt tgcaaaatta aaagattcct ttaataatac cttaaatact
    13621 ctacagggaa tcattgaaga agtaagtagt agcacggatg cagttgtaag cgctgcagag
    13681 gaagttagtt ccacagctca gagtttaagt cagtctgcaa gtgaacaggc ggctaatgtg
    13741 gaggaaacga gtgcttctct agaacagatg agcgcgaata tcaaccaaaa tgcagataat
    13801 gcgaaacaaa ctaatacaat tgcgactaag gcagctaccg atgctgtcca agggggggct
    13861 tcggtcctag aaactgttaa agcaatgaaa cagatcgcac agaaaattgg tattgtagaa
    13921 gatatagctt atcaaacaaa tcttttagcg ttaaatgcag caatagaagc cgcaagagca
    13981 ggtgaacatg ggaaaggctt tgccgttgta gcttctgaag taagaaagtt agccgaaaga
    14041 agtcaatttg ctgcgaatga aatttcagat ctagcttctt cgagtgttca gattgcagag
    14101 aatgccggaa aactaatcag tgatattgtt ccatctatcc ataagacagc tgatctagtg
    14161 caagaaattg cagcgagttc tgccgaacaa gcttcagggg ttggacaaat taataaggca
    14221 attactcagt tagattccgt aacacagcaa aatgcttcag cctcggagga gttagcgtca
    14281 acatcggagg aattgtctgg gcaagcagaa gcgttaagac ttgctatgac tttttttaaa
    14341 acaggaaaag aatcacaaaa agaaataaaa ccagagaaag tgagttcggt taaaaaaacg
    14401 atagctccaa agactaatgt agtaaataaa aaaagtaagc cgattccagt tgaatctagt
    14461 cacgactttg aaaaatttta agaggaaaac atatgcaatc taaagtaata gaagaaagtc
    14521 aatatctaac ttttttatct ggtagcgaag ttttcggtat tggtatatta aaaataaaag
    14581 aaattaagga atactcaagt gttacaacga ttccaatgat gcctgagtat gtaaagggtg
    14641 tgataaacct tcgaggaaac gttgtaccta ttatagattt gctggttcga tttggtagag
    14701 gaaagagtac tatatcaaaa agaagttgtg ttataattgt tgaggttgag catgaagaag
    14761 aatctataga tataggtatt ttagttgatg cagtcaatga ggtagtcgat atacctccga
    14821 catccattga accagctcct agttttggag caaaaatacg cattgatttt attcaaggaa
    14881 tagggaagtt agagaatcaa tttgtaattc tactgaatgt gaacaaggtt ctttccattt
    14941 ccgaactatc ctcaatagaa gaaaaattaa catcagatat aaacaattaa tattaggaga
    15001 tttaggtcgt tatgcagaat acaaataatc aagaaactaa gtttagtata gctacaattg
    15061 gatttgtagt tattctgtta ttgattacat caatagtttt ttatcaaatt gatagattag
    15121 gtaaattgaa tgaccaatta atcgaacagg atatgcaggc tgtatcaata ttaaaagatg
    15181 cattgagcac ggctcgtgat aatgcgattg caacggctga atattttcta gactccgata
    15241 aaggtaagca tgcaattctt tctgagagga tcaaaaaaaa tagatccact attgacaaga
    15301 agcttgaaac tctagaccga ctgattcttg attctgaaaa caaagataag ttaaataaat
    15361 tagttacgaa tagaaaagca tatattgcaa tctctaatga aattgttgat ttaatcacga
    15421 ataagaatga tattaaaact gcaacgtata aatacacaaa tgaactaaat cctaaattag
    15481 aacgttatat ggaaatatat gctgagttaa tgctaggttt agataaaatt acaaaacaaa
    15541 atcaagaagc caataaaaga gcattaagct tggctcaaac aatacttttg tctttcaatt
    15601 taattattat tggaatcgta acatttggtt ggttctggat tcgtggaatt ttacgcaata
    15661 tacaaaagtc tagcattgaa aacttaaagt ttagagttgc acttgattgc acaagcacaa
    15721 atcttatgat ggcagataac gatagagtta ttacgtatat gaataagtct attaagaaga
    15781 tgtttgctaa agcagaagca gatttaaaga aacaatatcc taattttaat ttagataata
    15841 tgataggaac aaatatcgat acctttcata aaaatccatc tcatcaaaaa ggactactaa
    15901 caagaatctc cgaagagtat aaatcagtaa tagaaatagg agaaagaaaa ttcactctac
    15961 tggcaaatcc tatcatagat tcaaatggag taagatatgg aaccgctgta gagtggtctg
    16021 atattacagc cgaattagca gcaaaagccg agctagaaaa aacaatgaat gaaaatctcc
    16081 agattcgcgt tgcattggat aatgttacaa caaatgttat gatggcggat aacgatagaa
    16141 atattgtcta tatgaataaa gccattgtat ctatgtttac aaatgccgaa tctgatatac
    16201 gaaagcaatt tccaagcttt agtgcagcca atatcatggg taaaaatatt gatatattcc
    16261 ataaaaatcc tgcacatcaa aagcaaattt tgaatacctt ttcctctact tacaatgcta
    16321 caattaatat tggtggtaga acatttacat tggctgcaaa cccagttgta aatgaaaaag
    16381 gggaaagact tggtagtgtg gtagaatgga aagatattac ttccgagtta ttggcaaaag
    16441 ctgaattaga aaaaaccatg aatgaaaatc tccagatacg tgttgcattg gatcatgtta
    16501 caacaaatgt tatgatggca gacaagaatc gaaacatagt atacatgaat aaatctatct
    16561 ataatatgtt tcataatgca gaaatggata ttagaaaaca attcccagga tttgatgcaa
    16621 agaatttaat aggaaaaagc attgactctt ttcatcgtaa tccaatgcat caacaacaaa
    16681 tcttaggaac tatagcgaca acgcataacg caagtattca gatcgcagga agatcatttt
    16741 ctttatcagc aaaccctgtt atcaatgata aaggagagag acttggaagt gttgtagaat
    16801 ggcaagatat aacaagtcaa cttgctattc aaaaagaaat tgatgatatt attacaggtg
    16861 ctgcaaaggg tgatttttct aagagaatta gttctgttgg gaaagaagga ttttataaac
    16921 aactaaccga aggaatgaac cgactcatgg aagttagttc cataggatta gaagaagtgg
    16981 ttcgagtttt aggtgctcta gagaaaggag atctaaatga aaagatcact aatgattatg
    17041 aaggaacttt tggaaaatta aaagattata cgaataatac ggttgctaaa cttacagaaa
    17101 ttattagtga agtgagaagg agcacagatg ctctagttag tgcttcggaa gaggttagtt
    17161 ctacagcaca aagtttaagt caatcctcta gtgagcaggc tgctagtgta gaagagacaa
    17221 gtgcttcttt agagcaaatg aacgctaata taaatcaaaa tgctgataat gcaaaacaaa
    17281 caaatgcgat agctaccaag gctgcaacag atgcagtcca aggcggaaat tcagttcttg
    17341 aaactgtgaa agcaatgcag cagattgcac agaaaatagg tatagtagaa gatatagcat
    17401 atcagacgaa tctacttgcc ttaaatgcgg cgattgaagc agcgagagca ggagagcacg
    17461 ggaaaggatt tgctgttgta gcgtcagaag ttagaaagtt agccgaaaga agtcaagttg
    17521 ctgcaaatga aatttctgaa ctcgcttctt ctagcgttca aattgctgaa actgctggaa
    17581 agttaattag tgaaatagtt ccatcgattc ataaaacagc agaccttgtc caagaaattg
    17641 ctgcaagttc ttctgaacaa gcatctggtg taaaccaaat caataaagca attacgcaat
    17701 tagattctgt aactcaacaa aatgcttctg cttcagagga acttgcttcc acatcggaag
    17761 aattatccgg acaagcagaa gctctgcgtc ttgctatgac cttctttaag ttggacgaat
    17821 tagaaacata tgctccatca caaaagaaaa caactcagat taagaaaatg caaaaaacta
    17881 caagactagt ggagagagat tcctctgatt ttgaaaagtt ttaagataga attcacttaa
    17941 agaaagaccg taatttagaa ctatttactt gaattctgtc ctagattcaa tacaatagtg
    18001 ctgattcgaa gtatgaatat accgcctcta ttagataagg agtttaaata ttttcaaaag
    18061 cttatatttg aattggctgg tctaaacatg actatagcaa agaagccttt gattggaaac
    18121 cgtttagcca cccgattgag atactacgaa ctagactcct atttagaata ttatgaaatt
    18181 gtagctgata gtagaaacaa atcagaaagg cagatttttg tagatttact cactacaaat
    18241 gaaactagtt tctttcgaga accaatccat tttgatttta tgaaatcaca aattttacct
    18301 aaattagaga ctcaaaatcc atttagagtt tggagtgctg cgtgttctag tggggaagaa
    18361 ccctatagca ttgcaatgct actggatgaa tttattaaga ataatagtaa atgggaaatc
    18421 attgcatctg atattagttc tagagtttta gaaaagtcta aaaaaggtct atatcccata
    18481 gacaaatcaa atatgattcc tgagcgttat ttaaagcaat actgtttgaa aggagttaat
    18541 tccaatgagg gttattttaa aataagtgat tatattcgaa acaaaattga atttaaatct
    18601 gttaatttaa atgagcattt tccaaaggat ttgggtaaat ttaatttagt ttttttgcga
    18661 aatgtaatga tttattttga taaaccaact agaattaatc taataagaaa gatttcagaa
    18721 atattgcagc ctggaggtta tctttttatc ggacattcag aaacgttatt ggaaataaca
    18781 gatgcactta tgatggttcg accaactata tataaaaaga aatgaatata cctgagcata
    18841 tacttgaaat atttttaatg ccaggagatt ttttctgggg agactcatcc actagaatta
    18901 gaactgtatt aggttcatgt atttctatct gtatgtggca tccagtgaaa agaattggtg
    18961 ggatgtgtca ttatatgcta ccaacacgaa atgtagattt tctaagagaa aatatacgat
    19021 taaacggaaa atatggggac gaagcttggg aaatgtttgc tagagaaatt aggaaagcaa
    19081 atactaaacc aagtgaatac attgtgaagt tatttggggg ttctaatatg tttttatcta
    19141 gagatgcaga tgtcccagat ataggagtcg gaaaccgaaa tattgaacta gcgcgaaaga
    19201 ttatcagcga ttttcaattg aatttagttt cagaaaactt aggcggaaat aaaccaagac
    19261 gtatacattt tgatatttgg agtggaaatg tttggctcaa acgacaggag aatgaataat
    19321 tgaacaagat tcagatagcg gtcattgatg attctgcttt agtaaggcaa gtattttctg
    19381 agattattca ggaagaatct gattttaatc tattgtttac agcatccgat cctctctttg
    19441 cgttagacaa aatgaagaaa gcttggcccg atgtaattat actagatgta gaaatgccta
    19501 gaatggatgg aattactttc ttaagaaaaa tcatgcaaga aagaccgact ccaatcatta
    19561 tatgttctac actaacgcaa acaaattcac ctacattaat gcaagctctt caaaatggag
    19621 cggtagatgt aataacaaaa ccttctgtcg gactaaaaga ttttttatct gattcaaaac
    19681 gacttttcat agatgcgata cgcgctgcgt cagtttctaa tcttaaaaaa ctaaatatta
    19741 aaacccttca aactggaaca aaaacttttt cggatattga tattacaaaa acaaaggttg
    19801 ttccagtaac ccagttaacc actactgaga agattattgc aataggaaca tccacaggag
    19861 gaaccattgc tcttgagttt attttaacgc aactagaagc aaattgtcca gggatagtaa
    19921 ttgttcaaca tatgccagag aagttcactg cagcatttgc ggatcgacta aataagctat
    19981 ctaaaattga ggtaagggaa gcttctgata gagacagggt tcttccggga ctagcattga
    20041 tcgcacctgg aaatagacat atgtctttaa aaagaagtgg agcgcaatat catgttgagg
    20101 tttcagacgg tcctctagtt agtaggcaca gaccttcggt ggacgtttta tttcgatccg
    20161 ttgcaaagca tgctagttcg aatgccgttg gaatcatcat gactggaatg ggagatgatg
    20221 gatctaacgg attaaaagag atgcatgatc agggtgcatt tacgattgct caaaatgagg
    20281 aaacctgtgt cgtatttgga atgccaaagg aagcaattaa gaaaaatgca gtagataaaa
    20341 ttgttggact agaagagatt ccgaatatta taatgaaata taggactgta tgaataaaag
    20401 tatatataga aaagaagaga taattcgacc ggaaggaagt ttaccattta taatagtttc
    20461 ctacacgtta gaagatgtgt attataaaat aaaagataat ttaacaaaaa tttattctga
    20521 tgtattgttc gaaagtattc aattggctgg ctggactccc tcagtttcag aacttggttt
    20581 tcatcctccc ggaaataaat ctaaaattct atccttcaaa agaaaagttc atcgggatga
    20641 acttcctgag atcaaggaaa aatgtataca aatagcaaat caaaacttaa aaaaagatca
    20701 atcaattcgt atgactcctg gttatcaaac tttatttaat acggtaactg catccataac
    20761 ggatgatttt cataaagtct atttgttcaa aggtgttttt ggagaagtag tttataaata
    20821 tgaaagtcgt cagttgcaat atatcgatac tgccccatta ttttttaaag atagtgatgt
    20881 tgtatattat tttacaaatt tacgtgagta ttttaaccaa ttgaatcaaa ttaaataaaa
    20941 gagtgatcat gaaaaaaaat atttttttta aactaattgt tttaacttat tattgcttag
    21001 tcctttataa ttgtaaaccc caggaattaa aaattgaaaa cattgaactt tgcgaaaact
    21061 ttgctagtga tggagtttgt agagaaaaat tagataactc tcatacttat gaaataaaaa
    21121 taaataaaaa taaaaaatac gaaacatggg aagcattaag caattatatg tattttcatt
    21181 ctaggcaaac acctggtttt attgttcgtt tcaatcgaaa atttaccctc acagaaagag
    21241 aaacactaca taaaacatac aaagccaagt atgaatttta tggttcaagt ggtatagtag
    21301 aaggattcga aattggtgat gattggattg gttcctttca atatctaggt agcatcgtaa
    21361 aggacaggca aaggcatttc aaggaagaga aaaattttcc ttatttagaa actgtctttc
    21421 cggcaagtat acgttttagt tttgattcag ctttagcaaa ggggcaagca gcagtagaag
    21481 tgaatgttcg attgaattac attgagtaat tctatgaaag taattcctcg attaaacaat
    21541 attttattag tcttttttct aatttcctct acagcctttt tagtctggaa agtgatttgg
    21601 cagaatttat atattttttc aaatagcgat cttcttttta ttccagcatt tttaaatgat
    21661 ttttgggaag gtcattataa ttggagagaa tggaatttgc ctccaagccc ctatttattc
    21721 cctgatttat ttttttcttt cttagtgagt ttattgactc agaatttatt tatttccttt
    21781 ttaatttatt ccattagttt ttcttttctt atagtgatcg gtttaacgat gataattcat
    21841 ccgatggatg agtatttctc ttttcaaaga attttaagag taacaacgtt ctttattgct
    21901 ttttggatta ttttagtatc taattatcct gaggatttgg ctttatttat atttccttct
    21961 tatcatggaa gttcatttat attcggacta atattttata gaattatatt tcaaattgat
    22021 aatattaaca agataatagc atttatttta ttatctggat tagttatttc ttctgatact
    22081 cagattgttt atacttttta tattccattt ctgttaacgt ctatcttctt aaaatcagca
    22141 ttacctgaaa aactatatat aaaagtattt atttattttg tagttagttt tgttgtcgga
    22201 agcttttttt tgaaattact cggctcgata aatatcttta gcgttccaag tattccagtt
    22261 tggaaagaaa tcaaaatgaa tatatttcaa ttaaaaatat tagaaaatat tcgaaatata
    22321 acgcctgtcg ttaaagtata ttttatggaa ttatattcgg ataagattct atttttttat
    22381 ttattaattg tttcagttcc actcaacctg tattatgcct ttaaaaaaac agaagtaaaa
    22441 actgaactca gatttttctc tctctacgta cttttggttt tatttgtatc tatttttaca
    22501 caaatgtttt ttggaatctg gggaggagca agatatgttt gggctatata tttttttcct
    22561 tatgtttcca ttttaattta ttttggaaac tcattgtcta gaatgctcac aaatggaaat
    22621 tatagaaaag tggaagggat gagaaaatat gtatttttac tttctaagga tagaatcaga
    22681 tctcaattga ttttgtttgg catcgtgatt atttttttgg ttttatttat ttttatgtat
    22741 ttaatcaaag gaagagtaga attaacagtg agccaacctt atcctccttt tgtatcttgc
    22801 ctagatgagt taaaaaagaa atatgattta acgtatggta tatcagatta ttggaatgca
    22861 aagcatgtat cttatctatc gaaagaaaaa ttattcgtaa atcaggtatt tgtaaactta
    22921 gaaaaataca attggattaa taatcgagac tggtataaaa gagacaaaga aaaccaaagc
    22981 atacactatc attttatttt gccagaacga ttagatataa atagaatcaa agagaaattt
    23041 gggagtgatt ataaaactga gatctgtgaa gggagagagg tctttgtatt tgaaaaggga
    23101 ttttattact aatgctcgaa gggggacttg aacccccaca ccttgcggca caagcacctc
    23161 aagcttgcgt gtctaccaat tccaccatcc gagcgcggct aatgtcagaa ttatctttat
    23221 aggaaatttg acaacatttt tcagattttt aatctttgta aattttgctt tttaatagaa
    23281 gaaacttagt ctcgaggatt ttgatatttg cttttgttta aaaaccaatg gattaaagat
    23341 aaatccttag cctttattag aattgcattc gaaataaaaa taagtaattt ttacagaaga
    23401 aagattgaat atttggcatt tgtatacata agccgagtag attatactcg cggctaacgg
    23461 tagatggaaa aaaactgagt aagattgtaa aactctatct caaaatgatt caaaaaataa
    23521 tttataggaa gagaataggt ttagaaaaaa gaaaacaaat cggtaaaatg agagaataac
    23581 gatttgtttt cctttttggt caattagttg atagagggcg agaaattaag acctaccaat
    23641 ttctcttgcg gcaaggcttc cttctaggat cgcgcgcttt gcatcaagac cagaagtttc
    23701 tttcgcacca ccaatgataa aaatttgttt atttggatat ttttccttgt aagaatttgc
    23761 gagagagtct tctttttcct gccctgcaca aatcaagata gaatcacact cgattacttt
    23821 ttcaccagaa gattttaaat caatcaccag tccttcttct ctgacttcct tgtaactaag
    23881 tgaagtataa aaatctacac cagaagattg aagttcttgt aagagtgccc aaccagtcgt
    23941 tgtgccaaga ccagatccaa ctttccctga tcggcgcaaa attgatattt ttctcttact
    24001 cgtatgtggt tgaattttta cttctgtaaa acttccaaca ttatacttag caaaatactc
    24061 atcgatcgta ggatcatggt cttctaaaag tttatgtgca gagtcacagg caattccacc
    24121 accaccaatg attacaactt tctccccaag tgtaaatcta ccgtttaaat aatctacata
    24181 acttcctact tttttctttt caagtcctgg gatattgaaa tttcgagggc gaacacctgt
    24241 tgcaaaaata acagcatctg gatttaattc gtctaacgtt tgtaaattac attctgtatt
    24301 taaattaatt tttacaccaa gtgcttttaa ttcattttca aaatatcgaa ttgtttcttt
    24361 gaattcaaat ttccccggaa tagaggctgc catattgagt tgaccaccga gtttatttga
    24421 tctttcaaaa attgtaacat catgtcctaa actcgctgca actctagctg attccatacc
    24481 acctggaccg gaacctacaa cgactactct acttttcttt ccttttggaa tttgggaaaa
    24541 ctcaatttct ctatttgctt ctgggtttac aagacaagat accattaatt ctttaaaagt
    24601 atgatctaag caagcttggt tgcaagcaat gcaagtattg actcgttctg gttggttggc
    24661 tttgatttta tttacaaaat ctggatctgc aagcatcggt cttgccatac taacaatatc
    24721 tgcttctcct tcttttaaaa tccgtagaat tgtttctgga atattgacac ggttagatgc
    24781 gataatcgga acaccagtca cttccttttt tattttttgt gcaatctttg cccaagcccc
    24841 acgagggact agcatactaa tcgttggaat cctggattca tgccaaccga ttccaatatt
    24901 taatgcgtcg gctccattgt ctctgagttg ctttgccatt tgaactactt cttcgaaagt
    24961 aggatttcct gggattaaat caattccaga catacgatag acgattggga agtcaggtcc
    25021 aaccatggat cttatttttt taagagcttc tactccaaat cgcattctat tttcaggact
    25081 tcctccaaaa atatcttttc tttgatttgt tacaggagag aaaaattgat tgatgagata
    25141 gccttcactt cccatcactt ctactgcacc aaatccaact tgctttgcgc gaagtgcaga
    25201 ttgaccaaag tcttcgatgg tcttccagca ttcttcttct gtcaattcat gcggaacaaa
    25261 acgattaatt ggagcacgaa tcgcagaagg tccaactaat tctcggtgat aagcgtatct
    25321 tcccgcatga aagagttgag cgcaaaatac ccctttgccg ctcagtgctt tgtttacctt
    25381 ctctaattct ttacagtctt cttctttttg gaagttaaaa aaaacgatat ttcctttacc
    25441 tgcatggttt acagaaattc caccagtagt aatcattccc acaccaccat caaaacgttt
    25501 tccataaaaa gcgatcattc tatctgctgt ctcaggtaaa ccttcaagcc ctaagtgcat
    25561 agatcccatt ataattcgat ttggtagtgt tgttttatta attttgattg gttcaaatat
    25621 ttctttaaaa tgcatttata taaatccttt gtatacattt ttattcaagg ctaaaaagcg
    25681 taaatcaaaa gatttgaaag attactacag atggatacgg atttaaagat ttgtattttt
    25741 ttatgaatag gtcgaatttg acttagtcca tatatgacta agccgcaatt gcgaaaggtt
    25801 tatgaacagt ttgattgagc tgcaatgctt catataaatg ggcttcctca atttcataac
    25861 taccctctaa gtcagcgata gttcttgcga cttttttaat tttatttatt ttgcgaagtg
    25921 aaatttcagg tgcattttgt aaatgttccc aatacttctc cgcatttgga tgaattggaa
    25981 aaattgtttt taaaaaccta ccatctacat ccccattgaa attatattct gaatttagat
    26041 atctttctct ttgcatttct cttgcttgtt gaataaactg ataaatacta tttagatcaa
    26101 ctatactttt tcggcgattt tcctttttaa atgcttctac tcgaatgtta atatcaattc
    26161 tatctagaaa aggacccgat aatttggata gatagcgatc tgctttaaaa tcgggacaaa
    26221 tacatttttt aataatactt ccataataac cacaaggaca aggatttgta gctgcaatga
    26281 gtaaaaaaga agcagggtag gtgatatgat agtttacacg agaaattgta attcgccttt
    26341 cttcgagtgg ctctctcagt gcctgtatca cctgtggttt aaattcgcct aattcatcca
    26401 aaaataatat accgttatgc gctagtgtaa tttctcccat tctagaatct ctgcctccac
    26461 cgacaatgga aacatcagaa gacgtatgat gaggacttct aaagggtctt tttacaaaca
    26521 aagattcttc cgagagtaaa gtttcgctat ttgatttaat tcttaatata tctagatatt
    26581 ctttctctga gagaggaggt tgtagatcag aggcaagtct tgcaaggaga gtttttcccc
    26641 ctcctggatg accaaccatt agaagatgat gttttcctgc gactgcgatt gtgatagcac
    26701 gtaacgcaag ggattgatca ttgtaaagtt caagatcagg gaaaaaaggg aggatggaat
    26761 catatctttt cccagtgatt tccttttctt ttgttcctgt tacaatagaa tttaattcag
    26821 atagatgcga aatagtatag atatcaatcc cttctacaat acaagcctca tatcggtttg
    26881 catgaggaac aatcactgcc ttaaaatttt ctttttttgc attgagaata atactaatta
    26941 gcccccttat cggttttata gagccatcta gtcccaattc accaaggaaa aggtatttgt
    27001 ctatatcaat ctttgattca atttgaccac tgagttgtaa tataagaaag gcaatagata
    27061 aatcatacca actaccgtct tttcttgcat ctgctggtgc taaattaact aaaatactct
    27121 gtaaaggaaa atcaaagccg ctgttttcaa ttgctacatg cactctctct gttgattctt
    27181 taatgctagt agctgcgagt cctacgatgg taaaacgagg gattcctcgt tttagattta
    27241 cctctactcg aattgttttt gtatttaaac cttctagatt agaactatag gcataaccca
    27301 tatttgcaaa catactttct cctcttctgg cgcatatcgt aatcgaatta tgtaaaaaaa
    27361 cgtgaattcg atataacatg aaagataaaa gaataccacc gatgaacacg gatggtttaa
    27421 ttttcttcct atttgtcggt cataatcttt ttaaattatt ttttaatcga actgatgtta
    27481 atttattgaa tgatttacta ttacacaatt gatatgaacc tttattagca aatgaaccct
    27541 ttacaggtct ttttatatat agggtaaaaa atctggctct gtgtgcgcga aaatgggaaa
    27601 aaaatattat tttaaaaaaa atatttcatt ttgagggatt gtggaaaagc aggactttca
    27661 ccacgaagtg cacgaaggac acgaagaaaa attgtaaagt tcctaggaat ttttcatctt
    27721 cgcgatcttc gtggttagat ttttttaaag tttttacaca gtccctcaag tagttcggtt
    27781 gtcgagcagt aagtttcgac ccttagggag aaacgctcat gctattgagt ttatcgagac
    27841 aaacgtgtaa acaaaatcct ttcaactatt aatcaaggtc tgagtgccta aattaaagcc
    27901 accactggta tcaatggatg tttccccatt gagagagagt gaaactccgg tcatggctcc
    27961 attatttaac caacttgcaa ttctatcata aatcggatca aaggatgtaa aaactccagc
    28021 tgtggtagta ttgatatctc gattagatcg gtcgaaattg tctttatctt tatcgggaaa
    28081 taacgtccca taaactccac cattgacttt accacctact aataatacaa agtttcctgt
    28141 tccgtggtcg gttcctccgt ctccattcgc tttgagttgt cttccaaatt ctccatagaa
    28201 catcaaaaca gaattcgcaa aaacattgga accccctgtt tgtttaaata aaacgtcaaa
    28261 cgctttattc gttccaaata gatcatcaaa ttgtggttct atatctgtct tttgattttt
    28321 atgggaatcc caacctccat aagctaggga agcaattcgc atattgagtg cgggaacttg
    28381 taaagcatca tacagatttg caatttgttt tccaaaggtt gtatttttta aaacaagtcc
    28441 tgtgttaccc gatttcgcat ataaattggc aatatcggta gactgtggat ttgaagtgct
    28501 tagaacagat ttgacactat ccgttaaggt taggagttta taatgctgtg ctgtgaactt
    28561 tttaaataat ggaaagttgg cattccaagt agcgtctgca tatcgcgcct gcatgtaggt
    28621 ttgtaaagca cgggctcttt tgaaacgtag atctgtattc gattctgttc caaggtcagc
    28681 agttctagtt aagccaaaac ttctagaatc agaaaaagag actggtttgt tcaatgcggc
    28741 agaagaggtg ctacagaatt gtctgagtcc agtcgtcatg gaaagtagtc tagcattgcc
    28801 atttgctgag ttatctaata cgttaatcac ctgacctccc cagccttgaa cagaagtttg
    28861 gagcggacta ctttgataaa gccctgtttg tgctaccatt aaggaattgg aatggtctct
    28921 actagaagaa tgttttacat tacaaagaat agctaccttg cctgcttgga tttgagaaat
    28981 cagccaaccg ttgtaactca cacccgatcc agaatctccc tttaaaactc caaaggtttg
    29041 accattcaat gtaaattctt gatagtcttt tgtatacgca ttgttccacg aattgaaatt
    29101 agcctgagaa gttccgcgac ttatcgttgc agttcgattg ttccaatatt tatatccata
    29161 actggttgta tcagagctag gctttggaac gattaagtgt agaaaatcag gtccaccttc
    29221 cagtaaaaca tgaattaagg ttctaccagg aacggaaagg ctcgcagctt ctagttctgc
    29281 ggttaatttt aatttgtcgc caagagagag tcctgctgtt gcaagagccg catattttaa
    29341 aaattgttta cgtgaaatca ttatttacct acctgaacaa atgtaaatgg atttgttaaa
    29401 ataaaatcta tcgcagcacc tacacgatcg ttgtcatcgt ttaaggcagt tgtagtcgaa
    29461 ccggttctaa aaagtgtagt agaggctgca ttgtttatca gagttgcatt gtttcctgaa
    29521 gtaatgctac tttctgctgt aatattgtct ttagttgaac aggcagatac atttacattt
    29581 ccacaaagca tataatttaa atcccgtcct gtagacaaaa tagatacgat atgcgctctc
    29641 tctaaagctc caaaataaga gtaagttgca tctccaataa tacgtcgcca taaaaatttt
    29701 gcaatttcat ctgctgtaat atctgattgt tttggtagaa agtttctata gtctttaatt
    29761 cttacaaaag aaccatttcg ccaaatacct gttgctccaa attgtcctga tacggaagta
    29821 ttatttgcat ttctaaaaac atcatctgta ttagatgctt caagtcctgt ctgtcctcca
    29881 aaaacatcat gatctggtct aaatggattt gtgttttccg atgcaatttc ccaatcataa
    29941 cgcacaagac catttaataa ctcattattg gaaacgactg ttaaattcgc atttaaaagt
    30001 gttctatcaa aggtagaaag atatttcact ctagtagtat tgtgaaacat acttgagatt
    30061 gcatattttc gaatgaactc gattaaattc ttttttgaca tactattcca gagagcttga
    30121 atgtttgcaa tctttgtcgt aatatctgaa cttgtgccta ctaacggatt acttgaatct
    30181 aaattttcat ctgctaaatt tgagattata atcaatggaa gtgctgcctt gctttctgga
    30241 tgagcgatgg agagtttaga gagacttttg attcttgctt ctgcagtgga tccttggttt
    30301 gtttgagcat aaatacttaa attgccagaa cagtgtttcg tggttccaaa ggttgcgata
    30361 tcagaaaaag aatcattacc caattgcact acttgtatat cagataacgc acgagctgtt
    30421 tgcggaattg tgatttgctc atgactatta aaggattctg cgtttccaga aggaattgca
    30481 tctttagttg ttagaccact tctatcggtt ccaactccta ggatgccaaa atagagttgg
    30541 tggtattctc tagcgaagtc ctcattccca gagaaaacac ttcctgtgca aacatttttc
    30601 ttatgattgt attgagtggc tactggtgcg gataatgcca tattagccaa aacatcttca
    30661 tagcctagat tgtctctaaa actcctagct aaatcatttg caactgtatc atagtgtctg
    30721 aacatttgca catctgttac atttttatct tggttgagtg ctagatgata attcgtttcg
    30781 aaaaaagcaa ttttttgtcg aaatggattt agaccacgtg taggaactgc ttgtgtaaaa
    30841 gttcttgcag gagagttgtt ccaaggaata gatttttgat cgaaatcaga gcgactggta
    30901 gcaggaccat ttgttccata aatatgcgat agccgcgaaa gagatccctc tatcggattg
    30961 atacttaaat ttccatcaga agatgcattg gcaagtttcg aatgaacagt ccacatacca
    31021 agcatttcta caattgcatt ggatggttcc atgtttgccc aggctgtaat atcagcgtca
    31081 gacgcagcac caccataagc gaaaacacgt aatacgtctc ttactgcttg ttcattccat
    31141 tgccctgatc ctgatccttt cgaaatagct cctgcaaaac ctctataatt tgcgggatca
    31201 tcacttgcac ctgataaatt gactgcctca atatataaac caggacgacc tggtctagtt
    31261 gcaagtctcc acaaaacagc ttttacaaaa tcattatttt cttggcagga agtgtttccg
    31321 cattttacta aattgcaatc ttctagaaaa aaaatactta tgagtaaaag gattatagga
    31381 gtgagtagga attgaaagga tttctttaga ttaaacatag aacctcttag aatagagcta
    31441 ccatagaaag tgcatttcaa aaatacaaga atttaataga aactaatcta ttaaaattcg
    31501 ttgcattttg aatcaaaaag attagtagct ttgaatataa aaaatattta tagagtagaa
    31561 agaagatgat ttaagatgca aatcattctt taatttgatt cttcttcgta attatattct
    31621 tcctcatctc catatttttc ctccgttcta ggagttggag ttgtttttaa tttatagata
    31681 agcaatccag aaataattaa aatatttaga attgtaaatg gaaaaatcat ttgatgatac
    31741 attccatata ctgctaaatt ggaagagata caagcgagac caacaccaat taaaacacag
    31801 gtttcttgcg aaaggaatcg aactagaaag ctatctggtt ctgaatactt tttactacct
    31861 agaactattt caacaacagg ggaatatccg aaaaaaaatc ctataaacaa aaagcaagat
    31921 tggaaaaagt aattcccaaa ttcgtttgta tagtgagggc taccaatgag accgcctaaa
    31981 agtgaccagc cataggagat caaactaaaa agaataaatg caacaagtcc atcttgcata
    32041 cttccatttg cttctaaaat acttagcatc tgccttggta ttaacttttg aaaatcaatc
    32101 agctctgatc tatcaaattg atctctttcc aaatcaccaa acaaaataaa ataatgccta
    32161 agcagtgaca aaacgataaa cgaaagggta aaaaaaagta aataaagtat aaattccatc
    32221 agtgtctaaa atgcctcatt cctgtaaata ccataataag tccatgttca tcagctgctt
    32281 tgatgacttc ctcatctcga atgctaccac ctggttgaat gatagccttt gctcctgtct
    32341 ttgcgatttg gtctacacca tctctaaatg gaaaaaatgc atcgcttcct acaaatgaac
    32401 cttctaaaga gagtcctgat tttttagctt tggttgctgc aagttctact gaatcgactc
    32461 gagacatctg tcctgcccca attccaagag tagaattctt atctgtaaat acgatcgcgt
    32521 ttgatttgat aaatttaaca acattccaag caaagagtag ggattgtaac tcttcactcg
    32581 ttggttgttt ttttgtaacc acttttaaat cttttaccgt tactaaatca taatctctat
    32641 cttgtaagag taatccatgg tgaacaggtc taagatccat ttcccctaac gcttctttga
    32701 aactttcaat ttcgattaag cgtatgtttg cttttttaga aaagacttct cttgcttcct
    32761 ctgtaaatct ttcagcaatc acaccttcta caaagttttc agtgatcttt ttcgccaaat
    32821 ctccatttac ctcaccactg actccaatga ctccaccaaa ggcagagacg ggatctgttc
    32881 tatgtgcgag tttaaaagat tctagagttg tgtctgcata ggcaatcccg caagggttta
    32941 aatgtttgat aatagaaact gtgtttttag gaagaagggc tgcaatgtga aaggctgcat
    33001 caaagtcgag catattgtta aacgatagtt ccttaccttg tagagccgaa aactcactcg
    33061 cagcatagac tggttcatag aaggctgctt tttgatgtgg attttcccca tatcttaaat
    33121 tttgcttttt cttaaaacca agcgttagtt tctcagggaa acgatctcct tttagtcggg
    33181 caaagtaatt cgagatagtg gaatcataca ttgcggtcac agagaacgct ttaatcgcaa
    33241 ggctaaagga tgtttcttca gaaacacttc cttgattttt ttccatctct tcttggacaa
    33301 ttgtatagtc agatatttct gttactacaa ctgtgtgttt gtaattttta gaaccacttc
    33361 gaagcatcga aggtccacct atatcgatat tttcgattgc atcttctaat gtgacgttag
    33421 gctttttgat tgtctctaca aatgggtata ggtttacgat gattagatca atggagggaa
    33481 tgttattttt ttctagatct tctttgtgtc ctggcatgtc tggatttcca agaagtcctc
    33541 catgaatttt aggatgcagt gttttgactc ttccagagag aatttccggc gagccagtgt
    33601 attcttcaac tgcaattgcc gtgataccat tttgttttag ggcggctaaa gtcccacccg
    33661 tagagagaat ttcgactccc ttgttattca gatattttgc gaaatcaata agtcctgttt
    33721 tatcactaac cgaaataagt gctcttttaa ttttaatcat gttactctct aataaaaacc
    33781 tttctaccct caatttgaat tttatcttga ataaaatact ctacggaaag gggcagaatc
    33841 ttatgttcct ctgcatgtat attttgagtc aaatcactta tcgtactatt cggcaatatt
    33901 tctacaatag attgcaaaat aattggacct gaatcgattc cttcctccac aaaatgagtt
    33961 gtgcagcctg taaatctcac accatattcc atggcttgtt caattgcctt gagtccttta
    34021 aaagaaggaa gaagggaagg gtggatattg atcattttgt tttcaaaacg ggatatggtt
    34081 gaacctttaa tgattctcat ataaccagca gttacaatga gatcggggga gagttttaat
    34141 aataattcct ctaaaagttc atgatatcta tctttttcat ttcgatagga atcaaatggg
    34201 atcacgtgtg taggaattcc aaattccttt gcaagagtaa gtgcaccagc accctcttta
    34261 tcagaaatga gaccagcgat ctgcacatct aaacttttat tttgaatgtg ttgcacaact
    34321 gctttgaaat tagaaccttt tccagaacat aaaaatacaa ttttcttttg catgctttac
    34381 ttattttaaa tgagtaggct ctttgcaatt cagaaattga atttctaaaa atgattttct
    34441 aaaagcctta tcctagagtt aattcaatac acccattttt taaaaaattt agaaaagtgt
    34501 gaaaaatgaa agcaatcaga tgattgtata gtatgaggga tcggatgaac attcaaaaaa
    34561 cacttaaatt tattctagct ctttgtctta tcagcttatt tcatttttca ttttttggat
    34621 ttaattttgg taaaacgaat ttaattgtgc atgagtgggg aacctttact gctactcata
    34681 gtgcgaatgg aaaaccgcaa tcaggacaga gattcgctgg aaggacgtta cttcccaaag
    34741 aagtttatac attagtggat acaacactga atccaaaaca aaaaaaatct ttacgtttta
    34801 gaacagagag tggttatgca gttgagaatg gttcagaaga aagagccttt catgggattt
    34861 caatcttaaa tgatacagtg cgaatggaaa ctcctgtttt atatttttac actgataaag
    34921 agttggagtt agatgtaaat gtgatttttc cgcaaggctc aatcggtgaa tggtatccca
    34981 agaggaattc aggagaaaaa atttcctttg atcatgtgga taagaaagga tttttagtta
    35041 aatccgatga aacatcttat acctccctag atttaaaaaa ataccaagga tccatttctt
    35101 ggaaggctac aatattatca ccgagtgaga atacgccatt cacgatcaat acaaaatcaa
    35161 atgaatggat atctcctaga atgactgatt caaatcttgt gaaaatagga aatgaaatag
    35221 aaaaatatat tttttatcgt gggattgcaa gttttgaaat tcccattcag actcagttta
    35281 aaaaaattga atcagaaaat tttctagaat ttaagaacaa gtattctgaa aaaatacctt
    35341 ttgtgtttgt cttaaagaat ccaacgaaca atcctaagac gaagactgtt ttgtggaaag
    35401 gaaatttgaa tccaaatgaa aaaaaagaat taaaattaaa tgacactaag cctatcgtat
    35461 tttctgagaa ggatataact gaatttaaaa tagcactcga atcagaaggt ctctatccta
    35521 aagaagcaga ggcaatgcta aatacttgga aagagagtta ttttgaaacc aagggaatta
    35581 caatcttttg gattgttcca gaaagtttga ttcaaaaatt acttccaatc gaatttagta
    35641 ttcaaccaca tgagttcaaa agagtattta tcggtagaat caaaattgag aactaaaaga
    35701 gaatgctaaa aaattagata ttctcaattt ataaaaatat ctcaaaaaaa tgattataac
    35761 tatcaggaaa acttagatga aattctaaat cacaaaaaag attctttctt ttctaaagat
    35821 aggaaagaat tattagattt tagaaataaa ctttatgagg attacaatga ttcaatctaa
    35881 taaaccacct gaattctatt gggatataca aggcgagtat attttttttc taaaggggca
    35941 tttgagtcaa tggtggcata gctcgtttcg ctttgcagga aaagatttta actgtgcgga
    36001 acaatttatg atgtatcaaa aatctgtatt gatgcaagat gaggagacag gtaaaaaaat
    36061 attagagacg gatgatccta aagagcaaca aagacttggt cgtctcatta aaaattttga
    36121 ttctaatctt tgggataagg ttaaatacca aatagtttac ctaggaaatt ttttaaaatt
    36181 ctatcagaac aaagatctaa gagaagtatt atttcaaacg gacaataaac aactcgttga
    36241 agtaaatccg aaggatatag tctggggtat agctttgagt atggagaatc cagatcgact
    36301 agatcccaaa aaatggaagg gtgaaaatct tttggggagc atacttacaa atgttcgaag
    36361 agagttacag atagggaatt aatctttttt atcagtcggt gaatacgttt gattctcact
    36421 tgacacaatt acttttaagt agaaaattct aatcattatg gcaaatgaat ctacgactca
    36481 gactggcttt gaacttacat ctaattctcc cgatgaccca aggatgcata aagcttgggg
    36541 atacataaca gctcttccaa gtgaatattt aattcacttc caagggggaa agatcaaaga
    36601 aaagtcaagt ggacaaggcg ctacttgttt taaatggcct agagatacag tttttattat
    36661 tccaacaagt ttaaaagaaa ttttatttga tgcaagtcaa ctaacgtctg ataatgtaga
    36721 cattcgaatt cattgtatgg tagtgtatcg tattgtagat ccaatgcgaa tttacaagtt
    36781 gattaatttt tcaaatcgac agagaggaga agaaaaatta gcaaggatga tagcagacat
    36841 ttgtagatct caaatcaaac ggtttattgc taaactagat ttacaaagtt gttatagaaa
    36901 aagaaaagaa gaaattgcag acgcacttgg attagagctt tctaatattg tttcgcattc
    36961 ggaaacaggc tggggacttg aagttgtaac gaccgagata caggatattt ttatacaaga
    37021 tcctgaaata tttaaatcaa tgcaaagctt atttaaatcc tctcaattga aatcgtctcg
    37081 gtttgctgaa ttagagacac aaaaagaaat tgaagtaagg gaaattgaaa aagaaagttt
    37141 actggcagaa tatagattaa aacaacaatt ggagcaacaa aaaaatgatg caaagataaa
    37201 agaaagtgct cttcttttag ataaacagaa tgaagagaaa aaattcgaat tagaaaaatt
    37261 aagagccgaa aaagaagaag aaattcgaaa ttacaaactt attcaggaat tagaattaca
    37321 gaggaaagaa gctgagtttg aattagaaaa atctaaatcc caagcagatg caattcgatt
    37381 aacgcacgaa gaagaactag aatatttgca aaagaaaatt tctgtcgaag cgaatttaag
    37441 tcctcaaagt ttagaaaaag aatttttaga aaaatctctt cctgttctag cagaagcatt
    37501 tgctaagaat ttgaatggga tgaattatca agtaatcaaa tcggacggtg caaatctaga
    37561 ttctccttta ttatttttta tacagcagat taaagagatt tttcagcaaa aaaagtaatt
    37621 tgcctttctt ggaaaataaa ctatataaaa acattaccac agagtaatta aggcatacat
    37681 ttaatctata actgaagtta tttgggagat aaaatcaaga atttgccaga tacttgaaag
    37741 aaagaaaaat aggattttga tgaatttttt caaactgtct ttcggaattt ccatctactt
    37801 ttaaaagaat ttttgtcata gaatagaaaa atattttttt aattcttttt ttctcttgaa
    37861 aaattataga aaatgggtta ttttctttct agctttagtt caagcagttt ggtattttgg
    37921 tcgaaggtta ttttgaatag gagtaatgaa tgtctttatc aagaaaatct gcatatagtt
    37981 ccggatatga tgcttataaa tcaaatgaaa tctctacaat gagtcaagga aagcttgtag
    38041 taatgctcta tgaaggagcg attcggtttt taaataccgc tattgagaat atgactccca
    38101 gaaaatatga gattgtaaat cgcaatgtta taaaagcaca agatattgtt accgaactaa
    38161 tgacttctct gaatatggaa gagggtggaa agattgcaga ggatttactc agtttatata
    38221 tttatatgaa aaaaagaatg ttagaaggaa acattcagaa agattctaaa tatttaaaag
    38281 aagtcgttcg aatgctgacc gaattaaaag gttcttggga agaaattgct aaaaaagaaa
    38341 atactgttcc tgcaactcca gttgctgcaa ggcctgcggg tggtttttcc attaaaggtt
    38401 agtgaattac tcacatatct tacttaaaat cttaaaatca aatcttcaaa aaatgcagac
    38461 ttttctatct tacggagatg gagagtctgc agtcaaaatt acatttcaaa acgaaagaat
    38521 tctagatctc ttagaacatc caaaagcaaa aaaagactcc gagtatgacg aactgaacac
    38581 acttgtttta gattgtattt ccattcagga aaaatgtgat tctatgcttg ttgcgatttc
    38641 actcgaatat aaaaaagagt taaataccca catgatcaag cgtcaactca aaagccattt
    38701 acaaaaaaat acttgagtct aaacttatat cttctcacat attcttatga gcgcaatgga
    38761 aacagatgat gcatttcaat tctttggatt ggatcgaaat gcaaccgaaa aggaaatgaa
    38821 acatcgattt catgaacttg cccaaaaata tcatccagat aaaggagaat ttaattctga
    38881 tgtaattttc ctagaactga tgaaacacaa agctactcta gagaaatttt tcgaaactag
    38941 aaacgaacac atagaggcat tcaaagaggc aaatacagat tattctctct ataaagaagc
    39001 caaacaaatg caaaatgatt ctattttaaa atactttaag actcgcgaaa aattcaacaa
    39061 ccaaacagcg cctaatgctg attttgaaat ccagctgaaa tcagatctag cagttgcaaa
    39121 aaccaaattc ctacaactat tacaaaaata tccaaactct atctgggtag ctgattccaa
    39181 agattccatt catagtattg atgtttggtt gaaatagtgc ctcgagttag tttgattttt
    39241 tttaaccact gatgaacacc gataactgaa cgatgaagct acgatggata tttaaatttt
    39301 cctatctttt atcggtgcta atcttttaat ttttctgtta catcgaactg gcgttaattt
    39361 atataattcg gttgtcgagc attctgtttc gacccataga gaaacattga gtttatcgag
    39421 aaacccctat ggcacgacta acgtatttga aggaagcgat ggattgattc gattgccacc
    39481 ttggttagaa ggttgaatcg ataatagtaa agtgcgaaaa gagatatatt gtccacttgt
    39541 tagactcgtt tgataagtgc agattcgatt actagcagaa ggaagttgtg tgacatccgt
    39601 tgctacatct aaaatatact ccaatggttg gtttggaatc acgctcggac cggttgtgca
    39661 gcttagacca gaatttaaat cagcaggatt gcgagcagcg ttttccgtat tccctacata
    39721 cagtttatat cctgctagaa acggttcctg gtttgtgact cgaactttaa tacgataggc
    39781 gccagttatt ttattattat tggagtctac aatatttgca atcgaaacta gaatcggcgg
    39841 aacggtttta gatttggaca cagaatagtt tgtgcaacca aacaatgaaa ttaagccaaa
    39901 caataaaata acatgaattc cacgtaaaaa ttgtttcacc accgacttgc actgagcctg
    39961 tcgaatgtgt gcataccgat aattgaacaa tgaagatacg atagataatt ttgaaatatc
    40021 gtatctttta tcggtgataa tcttccctat cggtggtaat cttttcattt ttctattaca
    40081 ttgaactggt gttaaaataa ataacttcat gagtctacca ccatttcgct tgaaattcta
    40141 ttcttgtcat ctagaaaaat tcaaaacttg tttgtaagat ttttatggta atagcttatc
    40201 ttcaaataat agaattaaac aggaattaaa taatcattcc agccatattt ttcagcggat
    40261 actcgattga tatggggaat atgatttagt ttataatcca tttttttgat ttcagggact
    40321 ttccacctga gttctgataa aaggcgattt ccttttttag tattacattc tctacaagct
    40381 gttacaatat tttcccaaga attaaattcg gaaggctttt tatcctttgg gatagattcc
    40441 cagcgactac gtggaacgat atgatctagt gtgagtttgc tatggggagg ttgtttacca
    40501 cagtagacac aggtgaaatt gtctcgctct aaaatattgt ttcgtgaaac cttggttttc
    40561 ttacgaggaa cttggtaata gttggcaaga agtataatat agggaactgg aatttttaat
    40621 ttttccgatc gaataaaggt atcctctgct gctttgatta cttcggcttt ttccaaaaga
    40681 accatacaga ttgcatctcg aacacttttg attgagattg gcaagtaact agtatttaga
    40741 attagaacgg gttgtgataa tatatccatt tacagttttc ttcttgctaa gtattttttt
    40801 aaaatgaatc caatgataac aactgctaaa ataacaaaaa tggtaatctg accttgatga
    40861 acccaagtca tgagctgatc aaaattgctt gcaccataat aacctaaata tacccaaaca
    40921 ggcacactga tgattgctgc taaaaaatcc gtaataaaaa aacgcaagaa ggaaactctt
    40981 ctagtcacgc cggcggttaa aaagataggc gttctaagcc ctggcataaa acgcgccatg
    41041 aacacaaccc actttccgta tttctcaaac tgtctttgca ctgtttcgta tctctctctt
    41101 gtaagaaatc ttgctacaaa tggatgtttg ataatttttt ctccaaatgt tagacctgct
    41161 gtaaatacaa ttgagtcacc aattaataca ccagccattc caactccaaa cataatatgt
    41221 tcgtttgtat ttcccaaagc agaaatgact ccacctgcta caagagaaac atcttccgga
    41281 acaggtaaac caaacccaca taagatcaat actccgaata ctgcgaagta tccgtagtca
    41341 gaaaaaaaat tgactagtag atttaagaaa tccatgacag gaattactat gttgccaata
    41401 aaggcaatac tacaagcaat aaattttatg ataaaaacaa tcgatgagtt atacattgca
    41461 gaagattcta ggatcgcaga ctacggagta aaacactatg agtctagaga aagagtctat
    41521 gaagaaaaag gacatgcctt tcgcctgcct ctacaaagag atcgggatag gatctaccat
    41581 tctcgttttt ttaaaagact tcaatataaa acacaagtct ttacaaattc ggaaggggat
    41641 aattttagaa caagacttac tcatacgtta gaagtctcag gcattgcaag atcgattgca
    41701 accaatctag gtttgaattc tcttttagca gaatgcattg cactcgcaca cgatctaggt
    41761 catgcaccct tcggacatgc gggtcaagat attttatctg atctaatgaa aggcaatgga
    41821 ggatttgaac acaacaaaca atctcttaga attgtgcaag ttctagaaaa taggtatcct
    41881 gaatttccag gaatcaatct ttgcaaagta actctttcgg gaatcatgaa gcatggtgga
    41941 gattacgcaa aatcagagtt gaactcttta cgtttacaag aaggacctag cttagaatct
    42001 ctgattactg atatatccga tgaaatagct tatacttgtc acgatattga agatggtttg
    42061 gaaatggagt atattactat ggagagtcta atgtatgtta gcctctggaa agaaatgtat
    42121 ctaatctcca aagaaaaata caaagatgca acaaaagact tacttgcaag agcaacgatt
    42181 cgaggaatac tcaatttttt agttacagat ctcaccacaa caacagaaga aaatctaaaa
    42241 aagcatcaaa ttaaaaattt taatgattta aaattagcat ggcgaaacaa atttgaaatt
    42301 gcagattttt caaaagagac agaacctcga ctgagagaat taaagaaatt cttactagat
    42361 aacctttatc atcatgaaat ggtgattcaa atgtctagga gaggccaagg tattattgag
    42421 aaattatttc actattatat aaagcataca gaagagattc cagaaaatta tcgcagaaga
    42481 atggaagaag atggaaaata tagagctgta tgtgattata tatccggaat gacagatcga
    42541 tatgcgcaaa ttcaggtaga ttctatttca tgaatgaaac ccttctgatt gtagagtctc
    42601 cctctaaagc cagaaccatt caaaaatatc taggaagcgg atacaaagta ttagcctctt
    42661 ttggtcatgt gatggatttg cccaaagagg atttgggtgt agagattgaa aatgatttta
    42721 ctccccattt tgagatcaca aaaaaagcta ggaaaaacct aaaagaaatt ctagcacaag
    42781 cgaaaaaagc aaataaaatc ttacttgcgg gtgatccaga tagagaaggg gaagctattt
    42841 gctatcacct atcccttcta ttaaaacaaa agccaaatct aaaaaatcgg gtcgaacttc
    42901 ctgaaattac gaagaaagtt gttttatatg caatagaaaa tccagttcca attcataaaa
    42961 atcgtgtaga atctcaatat gcaagacgta ttttagatcg aattgtagga tataaggtaa
    43021 gtcctatttt gtggaagtgt ttgggtaaat ctacgttatc cgctggaaga gttcaatcag
    43081 tagttcttag atggatttgt gaaagagaaa aggaaattga tctctttgaa aaaaaagaat
    43141 attggaaagt aaaagcgttt gtagaaacaa aaaaaggaga atcttttact ctaactctag
    43201 aggataaaga gaaaaagata aacagcatag aaagcttaaa acaaacttta gaatcacttc
    43261 tcaatcaaaa aaattcagaa gttaaagagg gaatcctaga tcttaaatcc ttaaaaaaag
    43321 aaatggaagt catagaaatt aagaaagagg ctaagaaaga atatcctccg ccacctttta
    43381 tcactgcaag tctacaaaaa gaagcaggtc gtaaattaaa attctccact aaaaaaacaa
    43441 tgcagattgc tcaaaaactt tatgaaggag tttttgcagg aagtgataat ggttttatgg
    43501 ggcttatctc ttatatgaga accgattcaa ctcgaatcaa ttctgatttt aaaacgaaag
    43561 cgaaaaattt catagagaag agtttcggaa aggagtttgt aggaaatgat tttattcgga
    43621 aacaaaaaaa agagattcaa gatgctcatg aagcgattcg tccgactgac atttcaatta
    43681 ctcctgaatc tttgaaagga ataataaata aggatgattg ggaactttat caattgatct
    43741 ggaaaagaac gattgcctct ctcatgattc ccgctgagat tctagaagaa agtatgaaag
    43801 caaaattaaa tggattcgtt tttactggtt caggaaaaaa gattttaaca aagggttatt
    43861 tagtattatc cactgattca aaagtggaga cttatcttcc tgaactcaaa gtaggagact
    43921 atctcgacct aatccaaata gaatcggaac aaaaatttac cgaaccacct cctcgttaca
    43981 cagaaccaac gttagtagaa aaaatggaaa aagaaggagt aggaagacca tcgacttatt
    44041 cgaataccat cgaagttttg tataagagaa aatatacaac gcaaaaacaa aaatcaatct
    44101 acccaactga attaggaatg gaagtgaatg aattcttaac gaaagaactt gcctacctat
    44161 ttacagatca tttcacaaaa gacattgaag atgaattgga taagatagcc gaaggttctt
    44221 tgaatcgaac tgattttcta aaaaaattct atcaggaatt ttctattaaa acaggacaaa
    44281 tctcaaaaca aaagtttgtt cctttagaca aaaaaaatca accgaaacaa caatgtccac
    44341 tttgtcagag taaagtttca cttcgaaaaa atcccaaggg tagagaatac tggatttgta
    44401 gtcgctttcc tgaatgtgaa tacatggctt ataaggaaac cgttaagtaa aatcaagatt
    44461 cttccgaaaa atgtaaaatg agacatacat tagttatttt aattttattc ctcacattcg
    44521 gaatcaacct ttttgccgat tcaaagcaag aagacaaagt agagttaatc atctccgatc
    44581 ctaaaaaagc agaaggatct tggggaaaag aggcagatga ggtagaagcc tttcaagatg
    44641 ttaaaatttt cgatgatcta agtgaagaaa atacaaaaca taggttagaa gaagcaaggg
    44701 attttttcaa ttcctcttta gctgtttata aggcaacaga aaaggcaatt aaagaaaaaa
    44761 aagagctcca tataaaagag aaagaaagca atcaacaaga taagtttgaa tggcagaaaa
    44821 aagccagaga aagcacactc gataaagaat ataagcgtat gtctttggaa ggtagaaaaa
    44881 aggcagtaat agagctagtt aaagccatga atgctctgga taaaattgag aatccagatg
    44941 cgattacctc tcccgttttt atcgatttaa aggcaagtat ctacagagaa tatatcaagc
    45001 atcaatttag attaaaaaat tacaatcaat cgagtgaagt cttggagatg tacattgccc
    45061 ttggggagca atatgaaaaa gaagcagaac ctcataaatt actcgcaatg tgttatgagt
    45121 cccaagaaaa acaaacaagc cgttataaaa aagactctgt ttccaatgaa tataaaatgc
    45181 taaaaaattg ccacttgctc cggtttgcag atttggctta tggagtagaa tcgaatcaat
    45241 ttaaaagaat tgaaaagaaa actgtgaagt ttaatcctac tgttccaaat gctatgacag
    45301 gcaaagatta tgcgattgct tgtggttttc taaaaaaaga cggaaataaa gaatcaaaag
    45361 aagataaaaa agaagtctta ctcaaaaaag acgaaattaa agacacaagc aaaaaataaa
    45421 tctataaata gaatcctcga ttgtaggggt aaagaaatgt tatccctaca ttccaatctc
    45481 ataaaatact tcggctaaga caaattaatc ccaatgctca tctaaagttt cctatgagtg
    45541 gaggattttc cattcattgc tatctcaaaa tccctttttt taaaagagcc aggttgaagt
    45601 aaaattccta tcactaggtt aaaatgaatt aatagaattg ataaaaaaat attgtcaatt
    45661 ttgggtaagg gattcttatt aacacttttt gaaatggatt gtatatgaaa aatagattat
    45721 tctttgtttt acttttaatt ttattgatag tgggttgttt gaagactaca aagagcttat
    45781 ttgatttatc aagtccatct gcttccaatc tttcacttat ctatttcgct ttaacaaact
    45841 gttgtagagt tagcggtaat ataactggat tcacaagtgg caccatggat attacattga
    45901 attatgcacc aacaagccag aatgcccagg cacaggttac aataaagctg aatgcaccaa
    45961 catcgaatta tttctttagt gagaaaattc cttctggata ttattacaat gtgtttgtaa
    46021 gccagcatcc ttcgggactt tcttgtagcg ttagtaacaa cactgcaact ataacagact
    46081 cattaacaaa tttagatatt aattgtgtga gtgcagctat aacttccttc tattctgggt
    46141 atcctgattg gatggattac gttaagcgcg atactcctac ctccaattca ctttccttga
    46201 ctggaactgc ctgcactgga acggaacttg gttggtataa ttcctgtgtg catgtaggag
    46261 agattcgctc tatcacaatt ccaaatttta cttcttgcac agacattaca gctaccgatt
    46321 ctctaggggt ttttaattgg acttgtaaag ctgccaataa tacagtgcag gttgtctcca
    46381 ctggcttaaa gtttgataaa ggtcttactg atttaattga ttttacaaac gttaagtgga
    46441 aaaatatcta tattgtagtc aattacaaag gatctatgtt actcacatcc gtctcaaata
    46501 cttggtggac aaatccaatt agtgtggcta actcaggtct agccgctggt gtgagtaaaa
    46561 caatttacat tgtaacatcg aattccacat cacagtatac aatttcacaa aacagagtag
    46621 cgatagttgt tagaagtggt tcttctgtag taagtggagg aggagatgca ctcactataa
    46681 gttctagatt taattggata gaaggtcctt ttattggttc tggatcgaat ggtttgaact
    46741 ttgcaggaac tcctagtttt aatgtaatcc gaaattctgt cttttctggt ctattcgcta
    46801 tctcaaataa taactctaaa tacaattact ttcataatat atctatctac ggttctaatt
    46861 taggtgctaa tatggcaaat cctgcgaata tcataaatgg tttgaatgtc tataattttc
    46921 taccaggtgg atcatttagt attggtagtt ctgatatgat ttttctcaat tcaattattt
    46981 ctggaaatag ttctacttct gttctcttag gtggatcgaa taattcaaac attctatata
    47041 atacaacact tgcaaatagt ggcaatactg gtatcaactt aagtgctgcc ttaaggactc
    47101 gtattgtgaa tcttgcaacg attagcaata atggggatgg tataacaact aacggttcta
    47161 ataatacaga atttgttaat acagtagctg catcgaacgg aacagaaatt agtgattttt
    47221 cttctactat gacttttaat ggaattttta aaactgcggt cgcttcctgt ggtatttctg
    47281 gtgggtctcc tggaattaca ggcacttgtg caaaagctgg ttctagcgaa acgtcacccg
    47341 ctactgttac aaatgcaaac atttcaaatt cttttgttgg aaaagttaca tcagattcct
    47401 caaatacaat tggagcatct ggaacagcaa atttgggaag catttcagga aaagattggt
    47461 atagctttca aaatctttat cgaagttggg gaataggaaa cgttgccagt tttccttcaa
    47521 gtgtgcttag gggaaattgt tcctccactt gccagatttg ggattggtct ttaaaaaata
    47581 cagattctgt tcttcgcaat gtaaattctt gtccttcggg aaataacgta ttgactcatg
    47641 cttggaatac aggatatgga agtcagtctt tatgtgatac aaattatcca agctctattt
    47701 tttctgctgg tgtttgctct acagtattct tacgaaatgc ggtcgaaata tttggagatg
    47761 gaattggaaa tgacaatggg ctttgtgagt caaatgaaga ttgtttttat actccaaata
    47821 ttggagccta ccaaggtcat ggcactttag tttctgcctc gcttacaact ttcaatacta
    47881 ataactgttc tgatattggt gttggtggga ctatatccaa tgtaaagtta tggaaatggg
    47941 atacaaatgg ttattaagac ctagaatatc tactagaaac agtatgaaat aaaattaaac
    48001 attcttttct tgcgatcgct ctaatgagtg ggtgctcgaa attataaaat aagagcctaa
    48061 tctaacaaga ggactttatg ataaaaatag cagatacgag atggttacca gaacataaac
    48121 tcattataac tgaattgaga ggaaatgtaa attcagaaga tgtagagaaa tggatttcct
    48181 ctctctatca ggcacttgac caaatagagg acaatggtgt ttttaaaatt tttataaatt
    48241 taatggattt tcaagcagtt gatttagatg ttcataaaaa gtatagagag attattccaa
    48301 gaacacttgg tgaatacgga tggaaagttg gttacgtaga tatgtttgaa agagccaaag
    48361 agttacagct ttcgaataaa cgcggaattc aatgtgtggc agcagtgcat gttcatcaag
    48421 acgaaacaaa aattcaaaag tatgaagaca attattcaag agagaatgaa cattatttta
    48481 cagatattaa aaaagcagaa gattggattt atagttatcc tgtttaaatt ttaaaagtat
    48541 aaatagtaaa atgagtgtca gttaaaaaag attttgttct aggaattgcg tcttgctctg
    48601 gatagaaaag ttgcaaccaa aggaaacaaa tcttttttaa tgcgaaaaac tttttttgca
    48661 agttttggat cttaagaacg aaggaatcaa aaaactcgaa gtcctagaac ggaaataaat
    48721 ttacagagag gtatagtgta aaaatctttg agaatgaaga attttctatt gattccattt
    48781 ttatttttat tgattcactg ccaaaaagac tttaacgctc cctatctttc tacggaatta
    48841 aaagacttag aaggaaaaaa acataagatc atagattata aaggaaaagt tctcatttta
    48901 gatttttggg caagttggtg tgaaccctgc aaaaaagcag ttcctgtgat tgagctttta
    48961 cgggaaaatg caagctctga agactttgtt tttattgcgg taaatacaga caacgatcta
    49021 accgagatcg agattcgtaa ggttgcaaac caatttggaa tcaaatatca gattttactc
    49081 gatccagact tggaactcgc ccgccaattg aatgtagaag gcctccctgg actgtttgtt
    49141 tttgataaag ccggtcagaa tatctaccac caatacgggg taaatccaga ggatgcgcat
    49201 gatctattaa aaaaaatgtc cgagtggcaa aaatagggaa tttttcactg gacgaaatat
    49261 tcttgccttg aattctcata tcagttgcgc ttgcgcagtc tttaatttaa aagaggatat
    49321 tgttatatca tgaaatttac aacatttgat aatcaaagaa tcgttcttgc ttccggtatt
    49381 cgcactccta tcggtcaagc agggaaatca ctcgcagata ttccttctgc agaactcgct
    49441 taccgagtta cagaagaaat ttttgctcgt agcaaagttt ctaaaacaga agtagatggt
    49501 gtagtagcag gtgagatcag ccaaagcgta tatactccta acgttgctcg cgttatttct
    49561 gttcgcgcag gaattccttt agaagcaagt gcaatcacag ttgccaataa ttgtgtttct
    49621 ggttcagaag ctattttgga tgcagcaaga cgtattatga ttggagaagg aaaattttat
    49681 ttagctcttg gtcttgaatc tatgtcgcag ggtcctttca tagtagaagg tgcaaaatca
    49741 gttacgaaat tagacacagt tgataagtta atgaaaaact ggggcgaagc acaagcttta
    49801 ggtgtgaaag tgattgattc aatcgatgaa ggtttgaatg atccaattcg taaaatcaat
    49861 atggcgatga ctggcgaagt cgtggcacaa cgctatggtc ttaaaaaagc ggagttagat
    49921 gactatgctt atcgttctta taaaaaggcg tatgactcta ttcaagcagg gaattatcgt
    49981 ccataccaaa tgccagttaa actgacagat gggacactac tagaagatga cgaatatatt
    50041 atgagtaaaa ctggtatggt agaaaatcca ggtcgttttg cgaaagcagg agctatcttt
    50101 gattctaaat ttatgagcat caaagaattc tacgaaaaat atggagaatg gattggtagg
    50161 ccttatgtag agggagaaac agaagccgct gttactctat tcaatgcttg tccacgttct
    50221 gatggagcag cagcaatttt tgtaactact gaaaaagaag cagctagact tggtctagat
    50281 gtgcaagctg tgattactgg ttggggaatg tatggtgtag atccatctat catgggagtt
    50341 ggtatggctt ggtctatgaa caaggctatc aaagctgctg gacttgactt caaagatatt
    50401 gataattttg aaatccacga agcatttgct gcaactgcaa tgggaaccat gagagaaatc
    50461 aaaaaatcgt ggggctttga tttagaagca gtagacaaag aaggtggaag agtcaatccg
    50521 aatggaggaa ctcttgctct tggtcatcca ctgggagcaa ctggtcttag agtattactc
    50581 aaccagatca tgttcttcaa acaaaaccca agtgcaaaac gttctgtaaa ctgtatttgt
    50641 gctggtggtg gagttgctgg ttctattctt ttagaaagac cgtaatccct ccttgcagta
    50701 atatgtaaaa gcaagatttt caccacgaag tgcacgaagg acacgaagaa aaattgtaaa
    50761 tttcctagga aattttgacc ttcacaatct tcgtgttctt cgtggttaga ttatttttag
    50821 cttttacaca atcccgtatt ctaccatgac agaaattcat ttcctcgaag acaaaattgt
    50881 atcggaagaa aacctaaatc tctccctcct tgaaatatct ctaaaaaatg aaatcccaca
    50941 tattcatgcc tgtggtggaa acgcaaggtg ttctacttgt agagtaatta ttctagaaaa
    51001 tctggaaaac gtagaaccta gaaatgaaaa agaaaagaaa ctagctgaaa ttaaagggtt
    51061 tgaaaagaac attcgactgg cttgtcaaac tcatatcact ggaaatgtga aactcagaag
    51121 actagtcata gacaaagaag atattgaaat cgccatttct gagaaaagtg aaacaactgg
    51181 aaaagagaaa aagattgcaa ttttatttag cgacatacgc ggatttaccg cattttcaga
    51241 aaaggctttg ccgtatgatg ccattcatat ccttaatcgt tatttcaatc gaatgggtgc
    51301 agcagtttta gaaaacgatg gttatataga taaatacatt ggggatggcc taatggctct
    51361 cttcggatta aaagaagaag atccagatat gatttgtctt aaggcaatca aagcagccct
    51421 ttctatgatt gaggagttaa atgacctaaa tcgttatctg aaagatcatt ttgatttaga
    51481 atttaaaatt gggattggaa tccactttgg aaatgcaatt ctaggggaga ttggtcatcc
    51541 aggaaagcga caatttacag cgattggtga ttctgtgaat cttgctagta ggattgaatc
    51601 tacaactaaa aaggcaaatg cagagtttct tgtatctagt gatgtttata gcattttaaa
    51661 gacctgtatc aacataggaa gagtctttga aactaaatta aagggtaaaa caggtgttta
    51721 tcgtttgtat gagatcttat ctgtaaaaga acatagactg agtccaatag aggcacttca
    51781 gtctagtctt tataaattta tgactctaga agatgcccct aaatttttaa gactggtatt
    51841 tcatgacgtt agtaatttta atgtggaaac gaaaacgggt ggggcaaatg catccatacg
    51901 ctttgagtta gaaagagaag agaatcaaga tcttagaacg gtttttacca aagtggaaat
    51961 cattaaaaaa gatttggaag aaaagggatt taatatttcc tatgctgata tcattgcaat
    52021 tggtggggct gtcgctgttt ataaaacagg tggtcctaga attcagatga gtttgggtag
    52081 aatcgatgca accgaagctg gaaatcaaat tcatattaca actcctgata ttgatataaa
    52141 agaattattg tatcgatttt ctctattagg attggaacct aaagaactag tcgttctttc
    52201 tggtgcccac acaattggaa aagcaaacgg caagcctatg acggatgatt tatttcgttt
    52261 tacaaactcc tattttaaaa agctccttga attttgtcat ggtgcgagaa atgaaaactt
    52321 ggcgatacta aattcagata tcgttctttt ggatatgcag gaaacgagaa agtgggtcga
    52381 gttgtatgca agaaatgaga ttcaattttt tgaagatttt tccaaagctt atcaaaaaat
    52441 tacctctttt ggtaaaatgg gtgtctaatt aaaaaaaagg gtgaataaaa attcaggaaa
    52501 aatttttctt ccagtttcaa tgttttttta aaaaaattaa cttgaacttt taatgaaaaa
    52561 ttattttcat gggttaatct ttttttgatt cgaataaata aacagtaatc aggagtaaat
    52621 agaaatatgg cgaataatct cgcagaattg tacagggaat cggctgaaaa atatggttca
    52681 atgccatcct tcttttctaa agacgaaaag aagcaatacc agccagtaaa ctataaagac
    52741 ctctatgaaa tgggtcttaa tcttggggaa gctttaattg aactgggagt tcaagctaga
    52801 gacaaagttg gttttattgc agaccatagg ttagaatgga cagtaacaga tgcagggatt
    52861 ctgttaactg gtgctgctaa cgtgccaaga ggaactgacg ttactgaggc agaattagaa
    52921 tacattttaa accactcaga agcaaaagtt ctcttcgttg aaaatgataa agttttagaa
    52981 aaatttaaaa aggtaaaatc taaaacctct gttaaaactg taatcatgat ggatcctaaa
    53041 gcaaaaggtg agtcaggagt attaaaactt tatgacttaa tcgagaaggg aaaacaacta
    53101 agatccggag gaacaaagaa agtagaagct agagtagcag atattaagcc agatgatcta
    53161 tttactctta tctatacatc aggtactact ggtcaaccga aaggcgttat gctcatgcac
    53221 tcgaacatga tgcaccaagt aaatgatgta gttccaacat tagaaattta ctcatcagat
    53281 cgcgttttat ccattcttcc tgtttggcat atttttgaga gagtatttat gtatgctgct
    53341 attagtgcag gggcatctac ctactttaca aacgttagag atttaagaga agattttaca
    53401 aaagctaaac caacatttat gggatctgca ccacgtcttt gggaaagtat ttatttaggt
    53461 atttacaacc gattaaacga tcctaaacaa acacctcctg ctagaaaagc aatcttcaat
    53521 cttgcttatt tcttttctaa gcactttcac gcttctgttc gatttattca aggaaatgag
    53581 gtggattatg aaggtagaaa tccaatcgta tccctcctta ccggaataaa atctttaatc
    53641 gttgctatct taacctttat tccatatgta cttttagatg ccgttgttct ttctaaaatt
    53701 cgtatggcaa ctggtggatc tctcagagca acatgttctg gtggtggagc attacaaaga
    53761 cacgtagatg cattttttaa tgatataggt gttaaagtga ttgaaggtta tggtatgaca
    53821 gaaacttctc ctgttatttc tgctagaagt ttcaaacact tggtaatggg ttccgttgga
    53881 aaagtagctc ctaaaacaga acttcaaatt agagacttca atggtgctgt gttaactcac
    53941 gtgaaactcg atggatctat cgaaggtaag cgtggagcaa aagggattgt tcacgttcgc
    54001 ggaccacaag ttatgaaagg atattacaaa aatccagaag caactgcgaa agcattaaaa
    54061 gacggatgga tggataccgg tgacttaggg atgatcaact ataaagatac cttaacctta
    54121 actggtcgtg ctaaggatac tgttgtatta cttggtgggg aaaacgttga accggtccca
    54181 attgagaata aattaacaga atcagtttac atcaaccaag taatggtagt tggtcaagat
    54241 caaaagaact tgggagcaat tatcgttcct gattttgaaa aattaaaagc atgggcaaaa
    54301 gagaatggta tttctgagtc tgatgaaaaa gcactcatag aaaatccaaa ggtagttgag
    54361 ctttataaaa aagaaatcaa agagttaaat agtgctaaaa atggatttaa gtctttcgag
    54421 caagtaactc catttttctt aattactaaa ccatttgaag ttggtgatga aatgacaaat
    54481 ctcatgaagc taaaacgtca tgtgatcact gaaaaataca aagaccaaat caaaaaaata
    54541 tatatgtaag attggtacta taaaaaccga ttttacttat ctaaaaagtc ttcgtctccg
    54601 cttaaaaacc ggagcacgaa gctaaatagg atcctttttc aaaatttgaa tcaaaattct
    54661 ctaagtcttt tctgaattaa aatttatttt tttacgcaat tctagaatat gacataaaac
    54721 tcataagagt ttaataaaca tttctagtta aatcccaaag gcattgaagt taaatccaga
    54781 gcaagaagca gctgtaagac attttgaagg acctctatta atctttgcgg gagctgggtc
    54841 tggcaagact agagttatta caaatcgaat tgtttatctc attcaaaaga aaaaagtaga
    54901 cccaaaacaa atagtagcac tctcttttac aaataaaagt tcaaaggaga tgaaagatcg
    54961 ggttcgaaaa ttattaaata aaaaagaaag tagggggttg actctttcta cattccatgc
    55021 acttggtttg aatattctca aaaaattcat agataaatta ggttaccaca atccatttat
    55081 actccaaaca ccaggtgatt tagaaggaat catacaagac cttctaaaac aaagaaaaat
    55141 agatacaaaa
//
LOCUS       DAOQOS010000021        54750 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_229_length_54750_cov_6.150580, whole genome shotgun sequence.
ACCESSION   DAOQOS010000021 DAOQOS010000000
VERSION     DAOQOS010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 54750)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 54750)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54750
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_229_length_54750_cov_6.150580"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1059
                     /locus_tag="PLF66_06500"
     CDS             <1..1059
                     /locus_tag="PLF66_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002974469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HMZ63728.1"
                     /translation="FILAKTISNPILLLLSATIEIAKGNFRVQIKPSTKDEVGLLTEY
                     FVTMGEGLEEREKVKSILGNMIDPVVVGEAMKDMQALKRGSEKQITAFFSDVAGFSTI
                     SEQLTSVDLASLLNEYLSAMTIILKKYNGVLDKYIGDAIVGIFNAPVDVPNHPLAAGM
                     ASIEMIAKLNELREYWVKNNLYTKEAQEMDVRIGLNVGLAKVGFMGTDALASYTMMGD
                     TVNLAARLEAAGKDYGVNILISESMNKEIETELFTRGLDLVRVKGKNEPVKLFELIGT
                     RSSVTPSLKESAEIYQNAFALYLKQDWDKAIEFFKQAEKAKGKKDKAIELLIDRCSYY
                     KTSPPGDSWDGVFTRKHK"
     gene            1064..4180
                     /locus_tag="PLF66_06505"
     CDS             1064..4180
                     /locus_tag="PLF66_06505"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HMZ63729.1"
                     /translation="MKRRPSKDSVITIGILLLIILIFSILLYFDMHQKLGLGENPIIG
                     EVTFKERSVHRKMDASVVWDEVENKTPVANKDTIRTLDYSDAVLMTKDGTKIRLSDNS
                     MIYIDLSEKNLNINFDYGSISTSREDGSESDTKIQIKSGDKTIQLDNGGAAKLSKNED
                     DHVSIQVEKGKATLALKGKEEILQTNQTAEIRDTKIDVKPIRFLLQFPPDGKYYSTNS
                     DSMNTEFKWNPLDKGSSAELEIAKDKTFKQSVKKQSGIKDGSASIALAPGIYYWRINA
                     KNPLGGKVEFSESRKLTIFAERSVVITTPSDGKVFTYSLKIPSIALRWTKNDFSSSYK
                     IDISQSPDFSKLVKSLDSFENYINTDIPAKGTYYVRVTTRPNIPDLNPVISPVSKFTI
                     EERQVPAAPDLVSPISNTGFGLDFIQRSRITFNWHDNKEFTNYELEISKSSDFSSVFV
                     RQNSKENFVSPEIKEQIGEFFWRVRGILPDGRKSEYSKVNTFRVTEPAKLKLLFPESL
                     SELDMNSDKSILFRWQRPEALGKFILEVSQSPDFSKNILTPEESIVTGFSKKVILASE
                     GKYYWRVRLLTRDNKEISKSEDSSFTILGAPTPKPITPSEGTNIDLSNQQSVDFSWSR
                     NEKATAYNVEIYDTTKGGKRLIGNITTSDTSTSYKDLSKFRDGNFLWAISILYETKDK
                     RTIKSPPAITKFTVQAPKVEPPTPIALQPLNGADLIMGEKEEVMFQWEKNEKAVYYQI
                     DVQEKSRERTKPVFQKQTTEPNALFEIPITVGEGVFYWDLFIFYKTWDGRVVRSNPIR
                     NEFSLKFPYRNPPMTVLTTPTGGQDIKPDDNWNVPLVWEPNERAISYNVQVSEFETNK
                     TVFKETLETTRTTFVVSDTAIKPGKYIWSVSINYKAKDERIVKSPSRKQEFNLVMPSF
                     EVPVSIAISPEADTVINPINVSEVEFKWEKSNSALLYTVELFEITDGRQALILKKDSR
                     ENSFLFKDIPRLTNGVYMWKLTIAYKDRKLKTIYTEPLMTEFYVKIPAEDMIEPPQIL
                     SKPRLYVE"
     gene            4170..5324
                     /locus_tag="PLF66_06510"
     CDS             4170..5324
                     /locus_tag="PLF66_06510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63730.1"
                     /translation="MWNRFLHVIILILSILGYSSLTADEQEIEWAPNKNAKAYKLEVR
                     DFSSKKVKVDKKIKETRYKIDKLDPGLYEYRIGIVNDKDIVAVYSDWSTLSIMQSFEP
                     EGSVEEIYYGGKDDKFQEIYITGNNFFEDTKIEIFSEQGKIPIKNLIRVSDRELRFVL
                     ELSNAKPGTYDLKIINPLKKVYVKKGFYLLGKTKLEAEKVVIEAAKKSQGQETNVDRK
                     YFVRSAFLPGWGQYSSGKDYDLKFRRVKGAILFSATIGLGVFAAYSYKEYILQKRATD
                     DANYLNNQFNYPYNSNITPLGFYFSNQFYANVEDLDNKWHQFTRTSLILIGVYIFNLI
                     DSYFFTGAPSNPTSLNGLLPNHQIRAYVSPDRNGFNQTGNSYSLEYSIRF"
     gene            complement(5321..5590)
                     /locus_tag="PLF66_06515"
     CDS             complement(5321..5590)
                     /locus_tag="PLF66_06515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63731.1"
                     /translation="MIDDISDIVGIVGVVIILISYILLQFEKIKAKSLSYSIINLVGA
                     VLILYSLFFNWNLASVIIEVFWILISLFGIVKALYTKHKHKHSHH"
     gene            complement(5785..6696)
                     /locus_tag="PLF66_06520"
     CDS             complement(5785..6696)
                     /locus_tag="PLF66_06520"
                     /inference="COORDINATES: protein motif:HMM:NF025616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HMZ63732.1"
                     /translation="MKQKFKRYFLYFLLSFIALFILRLIFGYIVYSDDALINESHTVF
                     QTNNQVGFELARKNYASEKSYDKGAKQSLPQGGIKFDQKYEKIGALSSTTNDFDNEEK
                     NTRDLIKKFKALIQYEESSGLEGKRHLNLGIGVNPDKFDEMISEIKKIGKLQSIQVNK
                     TDKTNEYSDLNAQKESLLKSREALISFKGKGGKIDELINLENKILEVEESIQKLGVKL
                     GEFDTENEFCTIKFSLTETGKPNKSFFVSFVKRVKTAFEWTVKYYLLLIALFTFAALG
                     ILIGLAILEKLKIFPDLLKKYLESDEK"
     gene            6797..7966
                     /locus_tag="PLF66_06525"
     CDS             6797..7966
                     /locus_tag="PLF66_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010578303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HMZ63733.1"
                     /translation="MQSHNSQTSVQLTFGSLADEKIKELQAIIDGITEPLILINESFL
                     IKRVNKATLNFIDQAEYADIIDQKCFSKLYNRDNICPYCPLSAKGADNTDFSEIFNIE
                     NTPNPELKREILFKYEGKNQNLNLAFFPMINKDRTVTSFVEKISNITQLKEKEEENLR
                     MRNLASLGIMISGIAHELNNPLTGISLTVQNLVNNLNHFAPEIVLNRLEMIQKDLTRA
                     ALIVSDIISFAKPEKSKLALADLVDVTIKAKETVQRLYPDLAKNVTWEINFEEEHIFY
                     FNPSKIERLFQNLFRNSLQAFDYAKGYIRVDIKKKKNATVITVEDNAGGIPETVLDKI
                     FDPFYSNRKEGGTGLGLSICHSIVKEHNGKINVKSSEDRTKFSISIPTVKSQSRK"
     gene            7963..8952
                     /locus_tag="PLF66_06530"
     CDS             7963..8952
                     /locus_tag="PLF66_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015678252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HMZ63734.1"
                     /translation="MKKILIVEDISSIRTAIKDVLALEYSVFDAKNYLEAVEILKSER
                     MDLVITDIKMPGKSGLDLIRYVKENYPDTLYTLITAYNINDYIKFAKDYGVWNIIPKY
                     SFLDLRFISVMVYKLLTGDIFGVEKYFDKDLIINTKNTNGKFETPPPKTVMFKTVKSD
                     KDREYLSHRIGKFLISKGAPKIINQVIEELTSNAMIRAPRDEQGKSKYQLELPARDLL
                     ISDEKVELSERDYFEIGYGIYNKTFIIVTRDCFGSLKKEEILNRLDRHINVDETTGLP
                     PGVNDSHGRGLYICREISDSIIFNIEKNVRTEVITFLDNSENKSYKSLSIYDK"
     gene            complement(8988..10109)
                     /locus_tag="PLF66_06535"
     CDS             complement(8988..10109)
                     /locus_tag="PLF66_06535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63735.1"
                     /translation="MAKIILTLVIMTLIGISCNRNHNDKDNKNKGAGRLLVYQTILSS
                     RNSSSSSEVGNAINSSTSATSSAMSSLSDGSNAVSFNQKNPLSNPWEEKLMSAIDFIP
                     KLFVNSLEAVTITNQNCGAQGTYSRSTSPGDFGVATAFLATWTYTTCQWRVTFRGISF
                     TPSFTLSGTLENSWTGLSTTSPYIQSGSVLSVAPNMTYTSTERNVSVTETGNGSQTSG
                     GSNNVAHTFTWSAYSGSFSSGNIASSFTLSSNLNRVGKSGNTTLFSHTITTPTSLSIS
                     MSQSNSTRTFVSGSHSVKHNLANFTVTQTYNNMVWDYKTCQPVSGSATITVTGSRNGS
                     GTLTITSNGVGTYSYTATTRSGSDVNETGTITFPGCNMN"
     gene            10274..10786
                     /locus_tag="PLF66_06540"
     CDS             10274..10786
                     /locus_tag="PLF66_06540"
                     /inference="COORDINATES: protein motif:HMM:NF019235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1564 family protein"
                     /protein_id="HMZ63736.1"
                     /translation="MHKTITNNKLSYPRNYLNLAEFSSTLLIPEKYFALFQKKILEHK
                     GIRNYISSLLKKYQLYISNGLIPGYSNVTTKYQDKGQNLQKVSFRPRPEDWAELKLYR
                     VSFGMSISAFLVYLLIADSVEFEKTVSNYLAGVGITVITNFNLTAKVYLWNTKTYYAT
                     VFQYRKNYYP"
     gene            complement(10866..11642)
                     /locus_tag="PLF66_06545"
     CDS             complement(10866..11642)
                     /locus_tag="PLF66_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004494483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HMZ63737.1"
                     /translation="MHSPERIICLTEETTELLYLLGEEDRIVGITAYTVRPEKAKKEK
                     PVVSAFISGSIQKIKNLKPDLIIGFSDIQAQFAHDLIKHGLNVLIFNQRSISEIYNSM
                     LMIGSLVGRQTETLHLIDMWKGKLRKIELERAANPYKPKVFFQEWDEPIITGIHWVSE
                     LIQIAGGIDCFADKKSGVLARDRYVSKEDVQKANPDCIIGSWCGKPVDFDWVYNVSEW
                     QNTPAIQNKKVFEIDSSIILQPGPALFLDGIDKLVDFIQS"
     gene            complement(11646..13904)
                     /locus_tag="PLF66_06550"
     CDS             complement(11646..13904)
                     /locus_tag="PLF66_06550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63738.1"
                     /translation="MRSILILILILVTGCLQVKKSPFDFSKPSFFNIGLFFNAFFGDI
                     TISGTITGFNSGTIVLQNNLGSDMQYPPATTYQFKVARNTAYSISVKTNPVGSICTVS
                     NASGTATSDVNNVNITCNVISVSPVYVTNGRNWNDYINRDFTKDIFSQTDSACSSSNT
                     GGYRTCVHAGEIRKLDIPSKTSCTNLTASDNLGALNWVCKTNSSGGVTFYSSALKKGK
                     YLSTLIDWTTNTWQALTITIKDGTTTHAVSNPLTFWTNAMNTTSPTSGFISGTVYLYS
                     ANVTAGQINTSTTDKVSIVFKPGQTYTIGTSNTGTPAILTSGNFNWIEGTLETKFYSY
                     SIQMGGKFSVLKNFKAQNCYAAGNCVGIYLNSASNNYFEDIVVANMGTGTTNGAIYLA
                     NSFNNFFNTIILANNQSTGVNSSASTGNIYNGIISAGNAANGVSFSGANDNSFMNATL
                     INNTGDGINPSSASSFFLNLASVNNGTAGVSGGPFSNPTMENISCISNQYGFNLSGGG
                     SFYGRGMIRLSNNTANCQMAAGYVGGGISSGGCPLSSPSDSITTSNSGSVSASTFIGV
                     PTSSISDSTNASFSSGSATYSASLDWLNFSNFFRAAMMPAASFPLASAQGNCSSGSCS
                     VWDFSLKSTDTTLRNANITGGGSGCPAANTMFAHIWSASSQAACNSVIGASFNGSTCS
                     STILRNAVELLNDGVGNDNGLCEANEDCLLTPNQGAYQGHGNLVSASTVSGCADPSSV
                     GIKLYQYESNGF"
     gene            14441..14824
                     /locus_tag="PLF66_06555"
     CDS             14441..14824
                     /locus_tag="PLF66_06555"
                     /inference="COORDINATES: protein motif:HMM:NF025887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HMZ63739.1"
                     /translation="MKKLLITKEDIIKIENELFSAQLSSNVEVLDKLLFDSLIAVTPN
                     GQIVTKEMDLSSHRDKTMMIEDASTHIDEIQIIEDIAITVVTMKAKGKMMGSPMEGHF
                     KYLRTWKLLDGQLKIIAASIMILPE"
     gene            15071..15514
                     /locus_tag="PLF66_06560"
     CDS             15071..15514
                     /locus_tag="PLF66_06560"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HMZ63740.1"
                     /translation="MISKKFLISFILMLSLSIYANTDEVLLYAAEKGDLSQVKTALNW
                     DGDVNASDRNGATALIKACENGHKAIVELLLKKGAEVNARDKTGASAILYASIVGDKK
                     IVELLIKYGADVNIKDVGGKSPLSEATQFQNREVIELLKTAGAKP"
     gene            15575..16789
                     /locus_tag="PLF66_06565"
     CDS             15575..16789
                     /locus_tag="PLF66_06565"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002974906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HMZ63741.1"
                     /translation="MLLVAKRLDVVEPSPTLAITARANALKAEGKDVVGFGAGEPDFD
                     TPEHIKNAAKLAMDKGQTKYTPVSGTVSLKDAIINKFKKDNNLVYDRKNIIVGVGGKQ
                     VLYNFFMATMNPGDEVIIPAPYWVSYADIVRLAEGTPVIVQTTPENNFQITPAQLEKA
                     ITPKTKVFIFNSPSNPTGAAYSKKDVEALCEVILKHNIMVISDDIYEKVIYDGLEFVN
                     PAMLSPEMKERTFVVNGVSKAYSMTGWRIGYGAGHPEIIKNMDTMQGQSTSNPTSIAQ
                     AAAEEALKADQACITEMVKAFDDRRKKIVKALNDIPGVNCRMPQGAFYVFPYITGVYE
                     LPGFQALLKANPNVSKSKLFCDVLLEKYEVAVVPGIAFGDDSAIRLSYALGQANIDKG
                     VARIAKMISDLK"
     gene            complement(16855..18231)
                     /locus_tag="PLF66_06570"
     CDS             complement(16855..18231)
                     /locus_tag="PLF66_06570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63742.1"
                     /translation="MEKDLERCKIRLIDSIKELGGKIDVENDEDINYISKLIIHSMTG
                     TWRYFHTPEHIFEVGGDKDPVEMLAALFHDMIYIQVDSGININLSGYLYPFVREDDNK
                     LYIINKPELLKNKTFQLLLLIFGFKLGDILSPYAGQNEFLSAMIAMQCLEPYLEMGAL
                     AEIAACIEATIPFRRLVDNKTPYDILKVRLEAVNSYYSLNWSEDRINETLKKALRMAN
                     RDVENFGNPNSVKFLDNTWNLMPETNHDLRTHTSYTIVGYRTSMLKMVNFLKTLQPEF
                     IFHQYMDEPSDEKLEKLFKDTRKNLELSRIYLESKILTVGILEALSFRIGRNIPLSTL
                     IGEIPVNGIVIPTLDYFLPPVENPYVPQNPIEEEALLILTKGRNKDTAYDLKNSPITA
                     FLVNAIGFPEIDKLFEISKGFYDNKISAEEFLNHCDPFVLESIINGIIKIFEIRTKNL
                     RRLKTELP"
     gene            complement(18322..19053)
                     /locus_tag="PLF66_06575"
     CDS             complement(18322..19053)
                     /locus_tag="PLF66_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010574181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63743.1"
                     /translation="MYKKNLLGGMILCITSILPSSVYALGTYAEGSVVAKIIQFESRG
                     ILFESFEGVMEVTKFDKEEKCDSAKDECFAPQKTKTNFSVRMDKPELINFISKNLNQD
                     LLLGYNIHRITPISLSSDMEILTAAKQESSPAPEQGEKLAVQKSGGKRNFSVTGKVLQ
                     LDYQGTIIGTYEGLYLDETRGKVHPFSVTNEQMAKYALAAMKSGGKYYLGISVAYATG
                     FRKSDYDLFEINYKEPAGGVNVKVN"
     gene            19230..20099
                     /gene="aroE"
                     /locus_tag="PLF66_06580"
     CDS             19230..20099
                     /gene="aroE"
                     /locus_tag="PLF66_06580"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HMZ63744.1"
                     /translation="MKISRDTKFFGILGFPLGHTLSPVIHNGLFEEYNFNGLYIVFEK
                     EKPSRNLFFGKDSIPLEGLSVTIPHKEWAFKQADHRDEASTHMQASNTLVRVQSKLNA
                     YNTDGYGAIRSIEEYKRNFFETGKGDILILGSGGSAKGISYSLVKRGLSAQKIVVAAR
                     NEKTGKALVQKLNSMQKEISEYATLESIQKESKRYSLIINTTPVGMKGKKDSQILSED
                     FFIKSHSLFDIVYNPLETPLVKLAKKKKVKIIPGYEMLLYQAMEQFRLFTGIRVDNKR
                     ISLVRKWLLKNLK"
     gene            20299..20838
                     /locus_tag="PLF66_06585"
     CDS             20299..20838
                     /locus_tag="PLF66_06585"
                     /inference="COORDINATES: protein motif:HMM:NF019235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1564 family protein"
                     /protein_id="HMZ63745.1"
                     /translation="MFKKFHEPITLSNSKLAFPRNDFNLSEFSSTILIPERYLELFFS
                     KAKKHTGVRNYIAYLLSKYQLFIANGLIPGCSNITTKYQDKGQNLQKVSFRPKPEDWA
                     ELKLYRVSFGMSISAFLVYLLIADSVDLVESLSDYLGGVGISIPPHFDLCAKVYLTQK
                     RTNYTIFFQYRKERKVRDN"
     gene            21181..22986
                     /gene="aspS"
                     /locus_tag="PLF66_06590"
     CDS             21181..22986
                     /gene="aspS"
                     /locus_tag="PLF66_06590"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020766875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HMZ63746.1"
                     /translation="MKDWVKESYSIRKWAGELGKEKAGETVFVCGWAFRFRDQGGVIF
                     IDLRDRTGIIQVVARKENIPEDFKLAEHVRSEYVLAVKGKLNNRDAESINPKIPNGKI
                     EVIIEKLEILNTSKTPPFALDEFDESGEDIRLKYRYLEFRKDELKNKMILRHKFIFAI
                     RNYLNEKGFLEIETPILNKSTPEGARDFLVPSRLHQGFFYALPQSPQIFKQILMVGGM
                     ERYFQIVKCFRDEDLRADRQPEFTQLDMEFSYIDQPNIISEMEAMVCKVFKESFNVDL
                     PSKFPQMSYAEAMDSYGSDKPDLRFGMKLYNVSDIVKNCDFQVFKGAIEAGGIVKAIC
                     VKGGSIISRKEIEDLTHWLSKDFRAKGLAYMKHGKDGLESTITKRFTPEELSGIAKAV
                     GSSEGDMVFFGADKISIVNASLGALRLKMAERFEKLDPNQYHISWVVDFPMFDVDDTT
                     KELSAIHHPFTAPFDEDFNINDSIEELKSKASKMRSKAYDLVLNGNEIGGGSIRIHKE
                     EMQEKVFQLLGISKEDAEKKFGFLLEALRFGAPPHGGIALGVDRIMMLMSGSKSIRDV
                     IAFPKTQKGTCMMSDCPTPVDDKQLEELKIRVVKV"
     gene            22986..23945
                     /locus_tag="PLF66_06595"
     CDS             22986..23945
                     /locus_tag="PLF66_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010418550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HMZ63747.1"
                     /translation="MPKEKSQPIQEKFEFNNEALYQKVCGINDSRLKDLEAYLGITII
                     PRGYSLIVSGQKDKVDIAMEFFRNLVDNYKKRPDKEDFDSFDLNYLIRQENHQDGWTP
                     NEKILTTFKGKQIFPRTKNQERFVNSLMKNLITFGIGPAGTGKTFLSIAVACRLLQAG
                     EIDRLVLTRPAVEAGESLGFLPGDLTQKVDPYLRPIYDALYECIGFERVQVLISLNKI
                     EIAPIAFMRGRTLNNSFIILDEAQNCTTSQLKMFLTRIGKNSRMSLSGDVTQIDLDRG
                     RSGLEKVLSILNNTPQIGVMHLEKEDITRHPLVSVIVDKFEGI"
     gene            23978..26380
                     /locus_tag="PLF66_06600"
     CDS             23978..26380
                     /locus_tag="PLF66_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020764488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HMZ63748.1"
                     /translation="MANTTDVLTKVRPIYFVRQLQFYLVLVTLTMVTYFLASPYLGQE
                     KTDMDDENSYAVGNVSPETIISNKEIIYDDLDKTKLKRAEAYKSASLIFERDYSILIN
                     QIQKYIDEDFENLKVIASEGSNNKNIGFLIYKNIRWKFRSKEDLEFLLQYPRKEKLRE
                     LVQKGTNLVFSSYCILKEISPEVMSSLGSGAHIINKGAKENTSTLDSSHVFPRTAIYQ
                     NQKVQEQLSKLLDEKLTGIDTNALTITKRMILSSYIYSFPACTFKPEETEQEKQREVA
                     KVSIVKSKIAKNEVIVKKGELVTPEIRAKLELLNEHNSRANVNSILSILIIQIILVSI
                     IIVFLIKYDPKRLNDVSSNVILFSTIWLLTSYTFIISKFFYHPDNNFESIYYFGIFIP
                     IGMICMLIAFIFDEQLSIALGFYLSFFIFLCSQNNATSFIIAFSTTVVASMYGSKIRK
                     RIDFIKSGIYIAGVQILVATSGYLIDSRQYWVATSTGSLLSDIVRSNIFKIYVACIVN
                     GFACALMTQFLLPVYEYIFNIPTRFKLQELADTGHPLLQALLTKAPSTYTHTFMVAAL
                     SERAAQNLGLDWLLVRVGVYFHDIGKIPNAGFFVENQHLIPKKENIDKNNPGLAAKIV
                     IDHVIDGIEMAKKARLPREVISFIPEHHGTSTMAFFYHKALADLSPNQRKKIHKKDFM
                     YPGPKPQRKETAIVMIADSVEAASRSLEEITPQALDELIQKIINIKLAENQLDESGLT
                     LGDLSIIKSSFKEILLSSLHQRPKYPNSEDTKKLENKEDLKNKPVPKTPSAKKISRTK
                     TK"
     gene            26514..27143
                     /locus_tag="PLF66_06605"
     CDS             26514..27143
                     /locus_tag="PLF66_06605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HMZ63749.1"
                     /translation="MKFIYLIMISLLFSYCSSSPTKDEKPNNVEPTANVQKDDPKQKF
                     LDLAYATSKNIKDLSDKSIASKTKALLNEAYVLLKLKKNEESIAKFEEAFEIGTDAEA
                     YYKYGNALSNTMKLEESIKAYDIALELGYEKEYNAIYNKGCSYSLLKNEEEAFKNILL
                     AVDRGYTAIPYMEKDPDLSYIRSLPEWKSKYKEIKKHAKEREEVVKSKG"
     gene            complement(27150..28349)
                     /locus_tag="PLF66_06610"
     CDS             complement(27150..28349)
                     /locus_tag="PLF66_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000431253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HMZ63750.1"
                     /translation="MQRFFIFITIITILIGLMYYYVGSRFLEGTSISGSLRILFWVAI
                     FFLILLTPVSYFFSLFLEESEWQKSFSFIAFTSLGFLTILFSLVLIHDLSISIVNGFQ
                     AVFAFFRTEIITDQGMESSINRKDFFLHLIKPSLGLISISLTGYGVYKAVHKVDVKRV
                     DIPINDLHPDLENFKIVQISDIHIGPTIKKDFLEEVVRRINVLQPDIIAITGDLVDGS
                     VYKLKHHLEPLKNLKSKYGSFFVTGNHEYYSGVLSWLPEIRNLGIHVLLNQNHIVNSG
                     EAKLLVGGVTDYKAHTILPSHASDPEKAILSKEKTDFKLLLAHQPNSVFEAAIVGFDL
                     QLSGHTHGGQYFPGNLLIHLFQKFVAGLYKHGDIWLYVSSGTGYWGPPIRIGAPAEIT
                     EINLKRG"
     gene            28738..29622
                     /locus_tag="PLF66_06615"
     CDS             28738..29622
                     /locus_tag="PLF66_06615"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HMZ63751.1"
                     /translation="MKQILIVDDSETSILYLKMVLKDYNVVSAIDGNEMWERLRESIP
                     SLILMDVMLPDVNGYELVKQLSTTSQYKDIPIIFQTAKSSSDDLEKGFESGAIDYIRK
                     PVDKKELRARIKSVLKIKELEHQLFIKSTTDYLTEIYNRRYFFEIALKELESTIASNK
                     CLSIILLDIDFFKKINDSFGHDAGDFVLKKFADIIKNHIRPKDVFARYGGEEFILLFP
                     ESDKNTSISILEKIQKIIRENSFFFQDKEISFTFSCGIFSLEETVQNVTLDSLIQIAD
                     TRLYAAKHLGRNRIISSD"
     gene            complement(29746..31164)
                     /locus_tag="PLF66_06620"
     CDS             complement(29746..31164)
                     /locus_tag="PLF66_06620"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HMZ63752.1"
                     /translation="MEELKYEVIISGSEYGKRKTDVIPGLYKAEDGKFYLDAVELENI
                     SQQARNLNVLVDSARSIMAEMSLDSLLDLIIQDVTYVMNADRATLFLVDNERHELFSR
                     VALGSKEEIRIEYGAGIAGYVAESRQTVNIRDAYSDSRFSPENDLKSGYRTKSILCMP
                     VYSSTKEIIGVIQVLNKKYTDHFTEKDEALLGAYASLAGISLANAQAYDELQKERDSL
                     EVRVKERTKDLASALQKSDQLLLNILPAEVAEELKEKGEVTPVHFDHVTIMFTDFKDF
                     TRIVEDMSPQKLIKELDGYFTQFDKTIERFYLEKLKTIGDSYMCAGGIPRKGVTHPYE
                     ACLAALEIQSFMNHMKQVKEKMSDSFWELRIGIHTGPVMAGVVGEKKFAYDVWGDTVN
                     IASRMEVSGTPGNINISHNTYECVKDVFECEFRGEVDAKNKGKIKMYYLKRIKSEYSL
                     DKEGYVPNYNLLELLEKKRKEI"
     gene            complement(31291..31815)
                     /locus_tag="PLF66_06625"
     CDS             complement(31291..31815)
                     /locus_tag="PLF66_06625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63753.1"
                     /translation="MIKLYIAIFLLIITHCSSITKNAIPIGQAYLCITVERKDGEIPL
                     SQKEFQKVAILSGTVLIYSNRQENDCYFFDTLPANKVYSPYLVENTEVVGNWYQWKND
                     GYRSRGLYGKFDKSKTRKYTVSEREKVFLGRYKLIVTFSEDEREASLYRYAGSHATGR
                     LIIKNAEYEIREIE"
     gene            31781..32464
                     /locus_tag="PLF66_06630"
     CDS             31781..32464
                     /locus_tag="PLF66_06630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63754.1"
                     /translation="MSKKIAIYNLIIPTIFLVSCSYDHVNIFSTQFFESVPVSKKIDR
                     TVCINPVEIKNKNIIEYRKESRTNQLHIGLTESEFQAEKKEFVKIFSENFLSYLKSKN
                     IFSSIDLCEREKREYQLSITLQEYTITTHFPFHPVLAGVLLVPMFFITKESNLSIQFD
                     FKKSETTIASVTRKENRESSIFTINYYPPKTFSLDGYLNRQYKGDKIYIDYILDDAID
                     ELSTKIYSN"
     gene            complement(32638..34200)
                     /locus_tag="PLF66_06635"
     CDS             complement(32638..34200)
                     /locus_tag="PLF66_06635"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010575564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HMZ63755.1"
                     /translation="MTTRKIDPTENNKQRILDSIIIGTGFAGICMAIRLKQAGIDSYK
                     ILEQKDTIGGTWRDNTYPGAECDVKSHLYSFSFEPNPNWKKMYGEQKEILAYMNFCVD
                     KYNLRYKISFNTNVKGIYFKEENGYWEVETDSGEVLFARTVISGNGGLSRPSIPDLPG
                     LENFTGKVFHSAKWDHSFDLQGKEVAVIGTGASAIQIVPAIAPVVKTLNLFQRTPPWI
                     ISKQDRAISDEERLLYSILPFTQQIYREFIYWVLEIRILGLVINPEIMKAFEQAAREY
                     LKKSIKDPILREKLTPNYLFGCKRILMSNDYYPTLERENVNVDVSGILEVRENSILSK
                     EGKEYFVDAIIAATGFQGAEALAPFEVIGLNRQNLNEIWKKSGAETYLGTTVSGFPNL
                     FLMTGPNTGLGHSSMIFMIESQANYIVHSILNLKKRNLKYMNVKKEVQDHYNEEIQRR
                     LQKSVWAENCNSWYVTKTGKNTTLWPGFTMEFRLRTLFMNPLDYEFVAANESKPVDNP
                     FRSLQDLVFAFL"
     gene            34566..35195
                     /gene="lexA"
                     /locus_tag="PLF66_06640"
     CDS             34566..35195
                     /gene="lexA"
                     /locus_tag="PLF66_06640"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020774430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HMZ63756.1"
                     /translation="MKELTDKQELVLKYITNIIKEKGFPPTIREIGDEFQITAKGAYD
                     HLKAIEKKGYLKTNKNQSRAIELLRHSAEDGIPVRVMSIPLVGRVAAGLPLFVDENIE
                     EYIPVPDRMAKKGTVFALRVVGESMIGEGINDGDIAIIQQKEVARNGEIVVALVGDAP
                     AGSEATLKTYFKDGDTIRLEPSNPKFKTIRTKKDNISILGKLVGLYRFY"
     gene            35255..35545
                     /locus_tag="PLF66_06645"
     CDS             35255..35545
                     /locus_tag="PLF66_06645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63757.1"
                     /translation="MKSFLLFFSLFLFLIQSCAPKKDQVSDTDLKRLIDRVSMVRLSQ
                     NLDFDPSEGKPLKTDYQIFKEVCELFRLDSTQALEKLKSQHPELYKSIMAKR"
     gene            35542..36936
                     /locus_tag="PLF66_06650"
     CDS             35542..36936
                     /locus_tag="PLF66_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HMZ63758.1"
                     /translation="MKNKERYIWISLVAILSCSIFQPIERVKAISTEGEKYLQILHEV
                     ISFIETDYVESVDEKNLYIGAIKGIVSSLGDPHTRFMGEDEFKHLQEETRGSFGGVGL
                     EVTHSEGSILVISPYDDTPAMKAGILPQDRILEISGKSTEKMSIEDAVKLMRGPAGTN
                     VTIKVKRKTVKDPFLLTLTRELIKIQYLKSAYLENEKIGYVRLTQFMGRENNTGGEFK
                     RIVQEFAAKDAQGIIIDLRSNPGGLLDLAIELSDIFLRPDLDIVSVKGRGEKLVRVFK
                     SQDGQGKVLDVPVVVLLNGGSASASEILAGALQDNRRALIVGNQSFGKGSVQNIYNLP
                     YKTGIALTIQKYYTPSGVSIHKKGITPDVVVNQITPDNEDKVVMEKLSKTTLIQNFVK
                     DNPGYNEKNIKAFQDMLSKKGYKLNKTISRFLLKKENVIGEKSPVYDREFDPQLNKAI
                     ELLLNPTNPRPTSM"
     gene            36936..37943
                     /gene="tsaD"
                     /locus_tag="PLF66_06655"
     CDS             36936..37943
                     /gene="tsaD"
                     /locus_tag="PLF66_06655"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004755206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HMZ63759.1"
                     /translation="MFGIGIETSCDETSIGIVEDGSNLISLKIFSQIETHAEFRGVVP
                     EIASRAHLEKINILLEEAIAESKIDWKDLSYVAVTNRPGLLGSLMIGAQLARSISLVH
                     KIPIVPVDHLEAHLNTVQLEKKVPKFPYLGVLLSGGNSSILLVEDYGKMKLIADTSDD
                     ALGEAFDKVSSLLGLPYPGGPAVEKRANQHKPQENEKNPFPELLKDYPQERIEFSYSG
                     LKTSVLYYLKKNTIDENKINEVCYHFQNTAFQLVEKNILKAVKATRVKQVVASGGVLA
                     NETLRKKLTSLAEKKKLELFYPEKKILCTDNGAMIASLGYYLFKQGKIDSIDFKVSPS
                     R"
     gene            38016..38783
                     /locus_tag="PLF66_06660"
     CDS             38016..38783
                     /locus_tag="PLF66_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HMZ63760.1"
                     /translation="MKLKLNWVPNALSLGNLTLGFVSVLIASEASLSNPNNSQIFLLS
                     GIFIILAALFDGFDGMAARALNATSALGAELDTLADLTTFGIAPGFLTYKMILENFKI
                     DLFGSGELFPYGMLVASIFPICAAYRLARFTVAHDPASFTGLPSPIAGVIVGLFPVAF
                     PNNTISAYLTISSFIAVALLMVSKIKYSKPQVAIRGKFTKGRILIILGGFAVLFFFLG
                     LAKVPFVMYGLLIFYVSSGFISLIIQLIQDYYPDNES"
     gene            complement(38880..39170)
                     /locus_tag="PLF66_06665"
     CDS             complement(38880..39170)
                     /locus_tag="PLF66_06665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63761.1"
                     /translation="MMKMEILYYKKYEDVVLLSKKLIYGKLKFIGKKIELQNVYYGFG
                     KILNEDINTIDCNISGNGNILLIINNKMALYKEIDLPHVEFRTEINFDIRDQ"
     gene            complement(39525..39974)
                     /locus_tag="PLF66_06670"
     CDS             complement(39525..39974)
                     /locus_tag="PLF66_06670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63762.1"
                     /translation="MKKKFILLFFFLQCIYIDNYKLENIRYPETPYHSAFSPAHSEIN
                     EIYFREWCNQFCETLSTIVKRIPEGYFLEIRGKIDATELPENREKISLARAESFRNIL
                     IQNGIPDHKLKAVGDLNLEEYLGKDKFDPENRVIRFRIQKMETLSED"
     gene            complement(39995..41521)
                     /locus_tag="PLF66_06675"
     CDS             complement(39995..41521)
                     /locus_tag="PLF66_06675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63763.1"
                     /translation="MKLFLALCFLFLQALYSDDTEPQKKFNITGVMRFRGFALSRDTL
                     LSRQSSTTPYYNPELEYSNAAATGANIFNQEVAARSVGKQTTLSRRNEKLNYYDSRVL
                     LNFEFFTSKYFDGFAGVQIGDMTFGGSSISNTDRNNPSILGSGTGGELGQQTPVNIRS
                     NFLYINFKLKEYDFTSRYGIQFFSSPLGRLVFAIGSGALMTKGIPGYKLTLEGGWIRT
                     RERTAADLDGNGFNDKRQNVNVFFSKAVFKPVNHYTLEVYQYSSTDNDQTDPFRETGN
                     LYWFGLFNEWRFGVVTIMAHGIYNRGSVKALQSIRNYSEQEIYQHRKQYNISGGLWDF
                     QISYSYNPQIKLNLIGIGTTGRPGFSKDGTPTSYHAGGYRTLFPDFSISNIAIDFTGG
                     YALFSARNMTGLYEVGSFVNIIIPGPFELTLGYYHLYANKSPYIENNRDFNVFYNKDS
                     SNFMGREYNLNLRWNVLSDFQLLFRSGLFQVGDGLRALLDNRGGSYFKEAFLTAEYKF
                     "
     gene            complement(41525..42361)
                     /locus_tag="PLF66_06680"
     CDS             complement(41525..42361)
                     /locus_tag="PLF66_06680"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HMZ63764.1"
                     /translation="MLSRYIYVCIIILFLPFFVFANEDSQLYKILKKKEIVVSVGGDY
                     EPYYIQNPKPGYPGFEVELAKAFADYLGVNLKEVVPLLTFADHAKAIKSGRVDISFGN
                     SSSMKRGQELGFSDSYYISSPGALVNKSILPAESEGQVVTNKIFRNLYDLKSLSGLII
                     GVKNNTSNYDFVKDNFPRFTIKPYQTDEAALKALNDNEINCYVADNLYLEGLLQRNPA
                     LKIKMQAVVSPVVEKQLSISMKKYDLLLMNEANFFIREMKRTREIDRLKEKYFNNNDW
                     VR"
     gene            complement(42376..43662)
                     /locus_tag="PLF66_06685"
     CDS             complement(42376..43662)
                     /locus_tag="PLF66_06685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63765.1"
                     /translation="MNKTNKFIILISFLIFLFALEDIFLNITVDFDTKNVMRLETRIE
                     NEDLEEKPASLEIRKGIVFKIFFLNSLLKEVNDDYAPSDGKLEKTIEKIEGLLIGQKV
                     KLFLNVFLIALCFSTVVSVLNNAWFAIFLQRVLLSVAFLTFSYYLARSIFLSIHLPFI
                     GIPMLALHLPVYVIVCMSILHTFQKFFVTFFTKKALFFTTICLFGIGFFAGFFFLDTT
                     KSSLSNSNSLVLPSMVIGISAIIYGLVYVLYLWLVYRLSLYLKLVDTSDYKFNALLLA
                     SFNDEENNARPTIKALGAAEPKKESSFLKIAGHFLLIIFAGVFVGNVVYIPLFSLQKH
                     YTSQFGILLIVLLFLLCIFYVRNYYKIGKEEGLSVTRNILVSLSFLQYRFLKNLFFIV
                     LSTVGVLVFITCLFLLLTMDTVILKNYNIIDKTINL"
     gene            complement(43670..44620)
                     /locus_tag="PLF66_06690"
     CDS             complement(43670..44620)
                     /locus_tag="PLF66_06690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63766.1"
                     /translation="MASDLYFIKFVARDSIQRTFVVFAGIANKQKNTIERFETLVSHS
                     NELININSDYTYKDVVNRIQKFMFEGNHIQSATLTLINSFKNSFASESYLVEMMYYLE
                     YDNSEKLNELLKQNLSKVLGKGDYLINTIFHPIQRDEIDASSVREDIIDKAKDKDFVD
                     SLIPAGSLVGNFTYVLSPVTGTRVDELRIGDRIMIKFTPDNEVSNNVINLLSLKEEGG
                     GIRPVPATVIDIKKSPAEVDAVVKVTDGVFGRYSEKETTVKVKLAGFETLATTIKKKN
                     DEEYLEDKPKKQFHFKVGTIGVAAVVGFLIVAWILTYIFL"
     gene            44857..45537
                     /locus_tag="PLF66_06695"
     CDS             44857..45537
                     /locus_tag="PLF66_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000052667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMZ63767.1"
                     /translation="MSIVKIENLKKSYYISDKGVPILWDLNLNVGEGEIVSVEGSSGV
                     GKSTLLNILGAMDEFDSGLVEVCGTDLGNISETDKEKFRASKISFIFQHHLLLPDFTA
                     LENVMIPLLISKMNVIKAKQEATAMLERVGLGERLASFPSQLSGGESARVGVARALVS
                     KKQLVLADEPTGNLDRENSKNLMALILELQRDLRFSMILVTHDMELAAMATKRNKLLS
                     GKLQPIPV"
     gene            complement(45742..47340)
                     /locus_tag="PLF66_06700"
     CDS             complement(45742..47340)
                     /locus_tag="PLF66_06700"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HMZ63768.1"
                     /translation="MNIPNLNIKLFLLFCVSILYFVGCSISKGNVDTMFAVYLSKIKD
                     SVNTSSTAATNTQPNVLSVTPANNTVGFIIGTPITISFDKAIDTSTLTMISTDGLCTG
                     NIQLSTNNFTSCLGGILNISTDQMIASISLQTSFCVESSYMLQIKVLSGVKDTLGNAL
                     ASSYSGSTSFQTQQAVLKVGALGGTTVKAMAVSCNILFIGGDFTSVGSSARNRIAAID
                     LVNGFEIPTWNPNADNTVNTIALNGNTVYVGGTFLNIGGQARSRIAALDIVTGQATSW
                     NPNANNTVNTIAISNNLAYVGGSFNGGGSIGGQPRSRIAAIDLNTGIGTAWDPNCNNG
                     VINIIAVSGNVAYVGGTFAAANIGGQTNRNYLVAVDLSTGLTLPGWCATGAVSPVNTI
                     VATSTDVLIAGSFVGGLCGTTFNNFGSIQISTGNATPGFASGVNTGTNNITNAIAISG
                     SNIYVGGAFTGAASFVSLVRNYIGATDLTGNATAWNPNANNVVNTIITLGTAVLIGGN
                     FTSVNGGTVANRIAIIDINTGVKR"
     gene            complement(47741..49531)
                     /locus_tag="PLF66_06705"
     CDS             complement(47741..49531)
                     /locus_tag="PLF66_06705"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP and VCBS repeat-containing protein"
                     /protein_id="HMZ63769.1"
                     /translation="MKIPLRLKIIFSISILSITCIGEATYKNACDPKNRNFINGLIFK
                     SFTGDNTSLCGIKSLNNQSINSSTNVPTVTIQNLKDKSTVYTGFMIGTATDSLGVSKV
                     EVAFDSGNYSTAQGTTSWKMKLPSGSLTWKDGSKHIINVRAINTAGGISPVTSITVYK
                     DKNHDINGDGYSDMVASSIAVGPGVVYIFHSSGINGITATSTTAANTTITGETTTTFG
                     DFIVTNDLNGDGYADLVVSDISFSGSAGKVYFFYSSGTNGITTTLASAAPKSIVGGAL
                     GYLGYGLDTGDVNGDGYADVAINNYIASTGAVYVFHSSASGITANNLAGANTTIVGIG
                     SEDISNFVSMGDYNGDGFTDLSVSSITAPATNKGKVFIFHSSGSGGIIAANTTSAVAS
                     IAGTANQDSFGYSINSGDINGDGYADLIVGADQNGAGSPIGKVFVYLSNGPSGINVTP
                     HASYVGESSGARLGGFVWIMDVNADGFKDIILGSAYINAGDGKMYIFHSSSSGVPSVP
                     LTSANRIIIGEVGGGGRFAAGISGSDINGDGYGDIIATANVLNNTQDGRVYIMYSNGT
                     TGISNTTAASITTKINPVFNTNKRFGYQAY"
     gene            complement(49533..49844)
                     /locus_tag="PLF66_06710"
     CDS             complement(49533..49844)
                     /locus_tag="PLF66_06710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63770.1"
                     /translation="MGDDYGKSWMEIKESARKKMKIEDYKETVNANQNSIRNASGKQT
                     GNPLRVAKAIIQTAESNKPPLRLLLVAASLRGARNKILELQTDFDRWEETTLSDDHLK
                     E"
     gene            complement(50211..50909)
                     /locus_tag="PLF66_06715"
     CDS             complement(50211..50909)
                     /locus_tag="PLF66_06715"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HMZ63771.1"
                     /translation="MNTKKIAVIGLGDFGKELVKCLYDERHEVIAIDKNMELVEEIKD
                     VCTNAVCLDSTDESAMEAQGLEDFDYVILATADDFETLVITSDILRRIGVKEIIARYQ
                     TPLHIRILKMLGITHIFNPEERAAKNMAEMFSHANIKGSIILSEEYRIAELLVPEFFN
                     GKTIMEVKLKEKYNLLLITIKRHKNLSKNKRREDDVVLETLGIPSAETVFRARDIMVV
                     FGNHKDVEKFLSII"
     gene            complement(50915..54208)
                     /locus_tag="PLF66_06720"
     CDS             complement(50915..54208)
                     /locus_tag="PLF66_06720"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HMZ63772.1"
                     /translation="MAIFLRFFILFFYTTYLFSGPTEDLFEAVRKRSITDINRAIDSG
                     ADINGGNEWNTTPLMLATHFGAVSIMKALLDKKADPNAINYRNETALRIACQKNFVEG
                     IHLLIDSGADVNEEDVNGANLLMNYSDKHSGKTNELAIIKILVERGANINHQKEDGES
                     VLMARVKDKEIFDYLISKNANLNLRNKDGNTLLHISIENKDIELVKFLLEKGADVNVR
                     NFTGDTPLMQAIYFDQKEMIQMILEKKPDLNLRDSEGMTAIIHAIDKKQRDVIQPLID
                     RKSDLTVADNKGYTPLMYAILSEEQDIVDMFLEAGADIYAKSQKGFSVFMLACYKGNK
                     KLVQQLLEKGIEIEEPSQAYETALYNAIRNPKNRQYEIAQMLLDRKKKTKRGKYRDYG
                     VLSAAYYNDFKSMQYFIENHFDLNLRDSEGNTAMMIAAERGYADLVNLLLTNKARAKK
                     LNRKKESALTLAVKNNHWAVVQILNPAYKPPPKPIVPTKTVIKKEDRIFKPENQNLIE
                     AIQKEDLSRLISLLNSGEIQINEQYGEKGKSPLMYASFYGSFLCAKYLIQNKADLKLI
                     DSSGSDALLQILHFARLEKDNIIYPSHVFIARLLLANGADVNTKDYAGDSFLYSSAYY
                     NQYELVKTALFYKAKVNQANEKGLTPLHASAWHKNARIVSLLLSYNADVNARDLENRT
                     PLYMVCISKENEGEIIRLLLTKNPDIHIKAKSGLSALDLARANGYGKILSVVMKSVAE
                     EKALEESDKNQYEYLRKEFLDKPLNWKNDKGMSFLELAVRYEKISEVKDLLSKGAEIN
                     TYNLELETPLIIACDRGNLELVQMLLANKAEVNFSTKTGNTALMSAAFFGKPEIVKIL
                     LENGARASAKNEAGDNALFIAARYGHKAIVQMLLEKRVHVNVLNKHFGSSLMASVKGD
                     EFEIAEMILKKRANPHFKFSDGNNALMMAIERGNKEITRILLERKPRLAEENRKHKTA
                     LILAVENGHAEIVRSLLKSNANINEKLFDGSNLLHLSVLRGYRDVMRVLIDYPKKSQV
                     LYSNIDINEKDKNSLTPLMIAAALGDYDSVKYLLEKGADRNMTDIHGLTAMDYAKDLG
                     YKEIVGLLQ"
     gene            54275..54505
                     /locus_tag="PLF66_06725"
     CDS             54275..54505
                     /locus_tag="PLF66_06725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63773.1"
                     /translation="MDTYKPISCEFHDDLEAYSVLKKKLEIVYESESGEVLTEFGKIV
                     DLYTRDKAEFMLLDSGKEIRLDQLIRVDNPIG"
BASE COUNT        18591 a         9887 c         8849 g        17423 t
ORIGIN      
        1 tttattttag caaagacaat ttcaaatcct attttactcc ttctttctgc cactattgaa
       61 atagcaaaag gaaattttag agtccaaatt aaacctagca caaaagatga agtaggactt
      121 ctcacagaat actttgtgac catgggtgaa ggtttagaag agagagaaaa agtaaaaagt
      181 attttaggaa atatgatcga tccagttgtg gttggagaag ccatgaaaga catgcaagct
      241 ctcaagcgtg gttctgaaaa acaaatcact gccttcttct ccgatgttgc cggattctct
      301 acgatcagtg aacagttaac ttctgtagat cttgcatctc ttctcaatga atacctttct
      361 gcaatgacca tcattttaaa aaaatacaat ggtgtgttag ataaatacat tggggatgcg
      421 atagttggta tatttaatgc gcccgtcgat gttccaaatc atccactcgc tgcgggaatg
      481 gcatccattg aaatgatcgc aaagctaaat gaactcagag aatactgggt taaaaataat
      541 ctctacacga aagaagccca agagatggat gttcggattg gtctgaacgt tggtctagcc
      601 aaagttggat ttatgggaac tgacgcactc gcatcgtata caatgatggg agatactgta
      661 aatcttgccg cacgtctaga ggcagccggc aaagactatg gagtaaatat tttaatttct
      721 gaaagtatga ataaagaaat agaaacagag ctctttacaa gaggtcttga tctagttcgt
      781 gtaaaaggaa aaaatgagcc agttaaatta tttgaactca taggaactag atcttctgtt
      841 acaccttcat taaaagaatc agctgaaatc tatcaaaatg cattcgcact ctatctcaaa
      901 caagactggg ataaagcaat tgaattcttt aaacaagcag aaaaagcaaa aggcaaaaag
      961 gataaggcta tagaactctt aatcgataga tgttcttact ataaaacttc tcccccaggt
     1021 gattcttggg acggagtatt cacacgtaaa cacaagtaac tttatgaagc gacgtccttc
     1081 caaagactct gtcattacaa ttggaatttt acttctgatt atattgattt tttctatcct
     1141 actttatttt gatatgcatc aaaagcttgg tctaggagaa aatccgatta taggtgaagt
     1201 aacgtttaaa gaaagatctg ttcatcgtaa aatggatgca agtgtggttt gggatgaagt
     1261 agaaaacaaa actccagtgg caaacaaaga tacaattaga acgttagact actcagatgc
     1321 tgtccttatg acaaaagacg ggaccaagat tcgtctatcc gataatagca tgatctatat
     1381 tgatctttcc gaaaaaaatc tgaatatcaa ttttgactat ggatctatct caacatctag
     1441 agaggatggt tccgaatcag atacaaaaat tcaaatcaaa agtggagaca aaaccattca
     1501 acttgacaat gggggagccg cgaaactttc taaaaacgaa gatgatcacg tcagtattca
     1561 ggttgaaaaa ggaaaagcaa cgctcgctct aaaaggaaaa gaagaaattc tgcaaacaaa
     1621 ccaaacagca gaaataagag atactaaaat cgatgtaaaa ccaattcgtt ttctactcca
     1681 atttcctcca gacgggaaat attactctac aaattcagat tcgatgaata ctgaatttaa
     1741 atggaatcct ttagacaaag gatcatccgc agaattggaa atcgcaaaag ataaaacatt
     1801 taaacaatcc gtaaagaaac aaagtggaat taaagatggt tctgcttcta ttgcactagc
     1861 tcctggtatc tattactgga gaatcaatgc aaagaatccg ttaggtggaa aagtagagtt
     1921 tagcgaaagc agaaaactta caatctttgc ggagcgctca gtagttatta caactccttc
     1981 cgatggtaag gtctttacat acagtttaaa aataccgtct atcgctcttc gctggactaa
     2041 aaatgatttc tcttcttctt ataaaattga tatctcgcaa agtcctgatt tttctaaact
     2101 tgtaaaatct ctagactcat ttgaaaatta tattaataca gatattcccg caaagggaac
     2161 ctattatgta agagtcacta ctcgtccaaa tatcccagat ctaaatccgg ttatttctcc
     2221 tgtaagtaaa tttacaattg aggaaagaca agtcccagca gcacccgacc tagtatcccc
     2281 aatttccaat actggatttg gtttagattt catacaacgt tctcgaatta cttttaactg
     2341 gcatgataat aaagagttta caaattatga attagaaatt tctaaatcga gtgatttctc
     2401 ttccgtattc gtgcgacaaa attcaaagga aaatttcgtt tctccggaaa tcaaagaaca
     2461 gataggagaa tttttttgga gagtaagagg tattttacca gatggtagaa aatctgaata
     2521 ctcaaaggta aataccttta gagtgacaga gcctgccaaa ttaaaactgc tcttccccga
     2581 atctctttca gaattggata tgaactccga caaatcaatt ctatttcggt ggcaaagacc
     2641 tgaagctttg ggcaaattta ttttggaagt atcacaaagt ccagatttct caaaaaatat
     2701 tctaactcca gaggaatcta ttgtaactgg attttcaaaa aaggtcattc tagcatccga
     2761 aggcaaatac tattggagag taagacttct caccagagac aataaagaaa tctctaaaag
     2821 cgaggatagt agttttacaa tactcggagc acccacacca aaaccaatta ctccaagtga
     2881 aggaacaaat attgatttat caaatcaaca atcagtagat ttctcttggt ctagaaatga
     2941 aaaagcgaca gcgtataacg ttgaaattta tgatacaaca aagggcggta agagattaat
     3001 tggaaatata actacatcgg atacatcgac ttcttataaa gacctttcaa aatttagaga
     3061 tggaaatttc ttatgggcta tttccatttt atatgaaact aaggataaaa gaacaattaa
     3121 aagtccacca gctataacaa aattcacagt gcaagccccg aaagttgaac caccgacccc
     3181 aatcgctctg caacctctga atggagcaga cctcataatg ggagaaaaag aagaagtaat
     3241 gtttcaatgg gaaaaaaatg aaaaagctgt ttactatcag atcgatgtgc aagaaaaatc
     3301 aagagaaaga acaaaacctg ttttccaaaa acaaactaca gaaccgaatg cactcttcga
     3361 aattccaatt acagttggtg agggagtatt ctattgggat ttatttattt tttataaaac
     3421 atgggatgga cgagtagtta gaagtaaccc aattcgaaac gaattttctc ttaaatttcc
     3481 gtatcggaat ccacctatga cagttctcac cactccgaca ggaggacaag acattaagcc
     3541 agatgataac tggaatgttc cacttgtttg ggaaccaaat gaaagagcga tatcttataa
     3601 cgttcaggtt tctgaattcg aaaccaacaa aactgttttt aaagaaactt tagaaacaac
     3661 tagaacaacc tttgtagtaa gtgataccgc tattaaacca ggtaaatata tttggtctgt
     3721 ttctattaac tacaaagcta aagatgagag aattgtaaaa agtccatcac gtaagcaaga
     3781 attcaatcta gtcatgccct cttttgaagt tccagtctct attgctataa gtccagaagc
     3841 ggatactgtc attaatccta tcaatgtttc tgaagttgaa ttcaaatggg aaaaaagtaa
     3901 ttctgctctt ctttatacag tggaattatt cgagataaca gatggcagac aagcattgat
     3961 tttaaagaaa gattccagag aaaattcctt tctattcaaa gatattcctc gtttaaccaa
     4021 tggagtatat atgtggaaac taacgatcgc ttataaagat agaaagctta aaacaattta
     4081 caccgaacca cttatgactg aattttatgt aaaaattcca gcagaagata tgatagaacc
     4141 accgcaaatt ctatcgaaac cgaggttata tgtggaatag atttttacat gttattattc
     4201 taattctttc tatccttggt tactcgtcct taaccgcaga tgaacaagaa attgaatggg
     4261 ctcccaataa aaacgctaag gcttataaac tagaagtaag agatttcagc tcaaagaaag
     4321 taaaggtaga caaaaaaata aaagagactc gttataaaat agataagcta gatccaggac
     4381 tctatgagta tagaattgga atagtcaatg ataaagatat tgtagctgtt tattcagact
     4441 ggtctactct ttccatcatg caatcctttg aaccagaagg ctctgtagaa gaaatttatt
     4501 atggcggtaa agatgataaa tttcaggaga tttatataac gggaaacaat ttcttcgaag
     4561 atacaaaaat tgaaatcttt tcagaacaag gtaaaattcc tataaaaaat ttaattcggg
     4621 taagcgatag agaacttaga tttgtattgg aactctccaa tgcaaaacca ggaacttatg
     4681 atctaaaaat catcaatcca cttaaaaaag tctatgtaaa aaaaggattt tatttattag
     4741 gaaaaacaaa actagaagct gaaaaagttg taatcgaagc ggcaaaaaaa agccaaggac
     4801 aggagacaaa cgtagatcga aaatactttg tcagatcagc gtttcttcct ggatggggtc
     4861 aatattcgag tggaaaggat tatgatctta aatttagaag agtgaaaggc gcaatccttt
     4921 tttctgcaac cattggttta ggtgtttttg ctgcctatag ctataaagaa tatattttac
     4981 aaaaacgtgc aacagatgat gcaaattatc taaataacca attcaattat ccttataatt
     5041 caaatataac cccacttgga ttttacttca gcaatcaatt ttatgcaaat gtagaagacc
     5101 tagacaataa atggcatcag tttacaagaa catctctaat tttaattggt gtttatattt
     5161 ttaatttaat tgactcctac ttttttacag gagccccgag caatcctacc tcgcttaacg
     5221 gtttgttgcc caatcatcaa attagagcgt atgtttcccc tgatcgaaat ggttttaacc
     5281 aaacaggaaa ttcttattct ttagaatatt ctattagatt ttaatgatga gaatgtttat
     5341 gcttgtgctt ggtatacaat gcttttacaa ttccaaaaag actgattaaa atccaaaata
     5401 cttctattat cacagaagca agattccaat taaagaataa ggagtataaa attaatacag
     5461 ctcctaccaa attaatgatt gaataactca aagattttgc tttgattttt tcaaattgca
     5521 gtaggatgta ggagattaaa atgataacaa cccctactat cccaacgata tccgatatat
     5581 catcaatcat aataacttac cttactcaaa atctgcgaat catttaaatg aggtttcaga
     5641 tcgtcactga aaaatgataa tagaattaaa caaaaagaaa tagagaaaat ggaggaatta
     5701 caaaagcagg ctattgattt tattagttca ttcgcattct ctaagattct taaaatcatt
     5761 atttccctcc taaaattctt ataattattt ttcatcagat tccaaatact tcttaagcaa
     5821 atctggaaat atttttagtt tttctaatat agcaagacca attaatatgc caagcgcggc
     5881 aaatgtaaat aatgcaatga gtaataaata atatttaaca gtccattcaa atgctgtctt
     5941 aactctttta acaaaactaa caaaaaaaga cttattcggc ttgcctgttt cagtaagtga
     6001 aaacttgata gtgcaaaatt cattttctgt atcaaactca cctaatttaa cacctagttt
     6061 ttggatagat tcttctactt ctaaaatctt attttctaaa ttaataagct catcaatctt
     6121 tccacctttt cccttaaaag aaatgagtgc ttctctagat tttaaaagag attctttttg
     6181 agcatttaaa tcactgtatt catttgtctt atctgtttta ttcacctgaa tagactgtaa
     6241 ttttccgatt ttttttattt cagaaatcat ttcatcgaat ttatccggat ttacaccgat
     6301 cccaagattt aaatgtctct ttccttcaag tcctgaactc tcttcatatt gaatcaatgc
     6361 tttaaatttt ttaattagat cacgagtatt cttttcctcg ttatcaaaat catttgttgt
     6421 agaggataga gcgcctatct tttcatattt ctgatcgaac ttgattccac cttgcggcaa
     6481 actttgcttt gcccccttat catagctctt ttcactagca taatttttcc tagctagttc
     6541 aaaaccaacc tgattattag tttgaaatac ggtatgagat tcgttaatta gggcatcatc
     6601 agaatagaca atatacccaa atattaaacg taaaatgaat aaagcaataa atgagagtag
     6661 aaaatataaa aaatatcttt taaacttttg tttcaaggcc tttctccttg gatagcatac
     6721 tttcaaatga attctagatg gctactagaa tctaaattat cgttgaaaaa ctaaatcaaa
     6781 ataaaatcct tgaagtatgc agtctcacaa ttcacaaaca agcgttcagt tgacatttgg
     6841 ctctttagcc gatgaaaaaa tcaaagaact gcaggcaatc attgatggta tcacagagcc
     6901 actgattcta atcaatgaat cgtttttaat taagagagtt aataaggcga ccctcaattt
     6961 catagaccaa gccgaatacg cagatataat tgatcaaaaa tgtttttcta aactttataa
     7021 tagagacaat atttgtccct attgccccct ttcagctaag ggagcggata acactgattt
     7081 ttcggaaata ttcaatattg aaaatactcc aaatcctgaa ctgaaaagag aaattttatt
     7141 taaatatgaa ggcaaaaatc aaaacttgaa tttggctttc ttcccaatga taaacaaaga
     7201 tagaacagtc acctcctttg tagaaaaaat tagtaatatt actcagctaa aagagaaaga
     7261 agaagaaaac cttcgtatga gaaatctagc ttctctcggg atcatgatct ctggtattgc
     7321 acatgaatta aataatcccc taactggtat tagcctcaca gttcagaatt tggttaataa
     7381 tttaaatcat tttgcgccag aaatcgtatt aaaccgatta gaaatgattc aaaaagatct
     7441 aactcgagca gcactgattg taagtgatat cattagtttt gccaagccag aaaaatctaa
     7501 actagctcta gctgatttag tagatgtaac aattaaagca aaggaaacag tgcagaggct
     7561 ttatcctgat cttgctaaaa atgtaacatg ggaaatcaat tttgaagaag aacatatctt
     7621 ttattttaat ccttctaaaa ttgagagact atttcaaaat ttatttagaa actctttaca
     7681 agcttttgat tatgccaaag gttacataag agttgatata aaaaagaaaa agaatgcgac
     7741 agttatcaca gtagaagaca atgcaggtgg aataccagaa actgttttag ataagatctt
     7801 tgatcctttc tactcaaatc gtaaagaagg aggaactgga ctaggtttat caatttgtca
     7861 ttctatcgta aaagaacaca atggtaaaat caacgttaag tcttcggaag atagaacgaa
     7921 attttcaatc tccataccaa ccgttaagtc acagtcaaga aaatgaaaaa aatattaata
     7981 gtagaagata taagttcaat tcgaaccgca attaaagatg tattggcttt ggaatattcc
     8041 gtcttcgatg caaagaatta tttagaagcc gtagaaatat taaagtcaga aagaatggat
     8101 ttagtaataa ctgatataaa aatgccaggt aaatctggac tagatctaat acgttatgta
     8161 aaagagaatt atccggatac tctctataca ttaatcactg catacaatat aaatgattat
     8221 ataaaatttg caaaagatta tggagtttgg aatattattc ccaagtattc ttttttagac
     8281 ttaagattta tatcagtaat ggtttataaa cttttaacag gagatatatt cggcgtagaa
     8341 aaatattttg ataaagactt aattataaat actaaaaata caaatggaaa atttgaaaca
     8401 cctccgccaa aaacagttat gtttaagact gttaagtctg acaaagatag agagtattta
     8461 tctcatagaa tcgggaaatt tttaatttca aaaggtgccc ccaaaattat aaaccaagta
     8521 attgaagaac taacatcgaa tgcaatgatt cgtgctccaa gagatgagca aggaaaatct
     8581 aaataccaat tagaacttcc agcacgtgat ttactgataa gcgatgaaaa agttgaactg
     8641 tcagaaagag attactttga aattggatat ggtatctaca ataaaacatt tattattgtc
     8701 accagagatt gttttggatc tctaaaaaaa gaggaaattc taaatcgatt agatagacat
     8761 attaacgtcg atgaaacgac tggactacca ccaggagtca atgattcaca tggtagagga
     8821 ctttatattt gtagagaaat atcggacagt attatcttca atattgaaaa aaatgtaaga
     8881 acagaagtca ttacattctt agataatagc gagaataaat cttataaatc tttatctatt
     8941 tacgataaat agaagagcga agttctatgg aacttcgctc taaaacgtta gttcatatta
     9001 caaccaggaa aggtaatcgt tcccgtttca ttcacatcac ttccgcttcg ggttgtggct
     9061 gtataagaat aggtcccaac tccattggat gtaatcgtaa gtgttccact tccatttctt
     9121 gatccagtta cagtaatcgt cgctgagccg gaaaccggct gacaagtttt ataatcccaa
     9181 accatattat tgtaagtctg agtaaccgtg aaatttgcca agttatgttt tactgaatgt
     9241 gatcccgata caaaagttct agttgaattg ctttgactca tactaatcga aagactggta
     9301 ggtgttgtaa tcgtgtgact gaaaagtgtt gtatttccac tttttcctac tctgtttaga
     9361 tttgaactca atgtaaaact agaggcaatg tttccagaac taaaacttcc actgtatgca
     9421 gaccatgtaa atgtatgtgc cacattatta ctaccacctg aggtttgact tccattccct
     9481 gtctctgtca cacttacatt tctctccgta ctcgtataag tcatgtttgg agcaacactt
     9541 aagactgaac ctgattggat ataaggagat gtcgtagata gccctgtcca agaattttcg
     9601 agagtcccac ttagagtaaa actaggagta aaacttattc ctctgaacgt tacccgccat
     9661 tgacaagttg tatatgtcca tgttgctaga aaggctgtag ccactccaaa atcacctgga
     9721 gatgtagatc ttgaatatgt tccctgtgcg ccacaattct gattcgtaat cgttactgcc
     9781 tctaaggaat ttacgaataa tttaggtatg aaatctatcg cactcattaa cttctcttcc
     9841 caaggattac tcaatggatt tttctgattg aaggatactg cattacttcc atcggataac
     9901 gaggacatag cacttgatgt ggctgatgtc gatgaattaa ttgcatttcc cacctcagac
     9961 gatgaacttg aatttcgaga cgaaagtatt gtttgataaa ctaaaagcct ccctgccccc
    10021 ttgtttttat tgtctttatc attgtgattg cggttacaac tgattccgat caatgtcatt
    10081 ataaccaatg taagaataat ttttgccata ttcaaagtct cctatttaac tactctaatc
    10141 cttcctatcc gaaaatctag atgagagtgc catttattta tctttttttt gaactcatta
    10201 ttttttttca aaaacaccgc tcatccgatt cagaattttc ttcattataa atataaacct
    10261 gaaggagact caaatgcata aaacgataac aaataataag ctttcttatc ctagaaacta
    10321 tctaaaccta gcagaattta gctccacact tctcatccca gaaaaatact ttgccctatt
    10381 tcaaaaaaaa attctagaac acaagggtat tcgtaactac atcagctccc ttttaaaaaa
    10441 ataccaactc tatatttcaa atggtctcat ccccggttat tcaaatgtta ccactaaata
    10501 ccaagacaag ggtcaaaacc tacaaaaggt ctcctttcgt ccacgacctg aagattgggc
    10561 agaattaaaa ctctaccgtg taagttttgg tatgtcgatc tctgcctttt tagtttactt
    10621 actgattgca gactctgttg agtttgagaa aaccgtctcg aactacctag cgggtgtcgg
    10681 aattacggtg atcactaatt ttaatttgac ggcaaaagtt tacctttgga atacaaagac
    10741 atactatgca acagttttcc aatatcgaaa gaattattat ccctaataat taaaaattaa
    10801 gtaaaaacta aaatcagaaa aaagaggatt caaaaaaaag aaggcgaagc tatgtgctct
    10861 tacaatcaag attgaataaa atccaccaat ttatctatcc catctaaaaa aagagcagga
    10921 cctggttgta ggataatgct tgagtctatt tcaaaaactt ttttattttg aattgcggga
    10981 gtattttgcc attctgagac gttatacacc caatcaaagt ctacgggttt accgcaccaa
    11041 gagccaataa tacaatcagg attagctttc tgaacatctt ctttagaaac gtatcgatct
    11101 ctcgcaagga cgccggattt tttatcagca aagcaatcta taccacctgc aatttggatg
    11161 agttccgaca cccaatgaat tccagtgata atgggctcat cccattcttg gaaaaaaact
    11221 ttcggtttat agggattagc cgctctttcg agttcaattt ttcttaattt acctttccac
    11281 atatctatga ggtggagagt ttcggtctgt cttccgacaa gggatccgat cataagcata
    11341 gagttataaa tttcagatat agatctttga ttaaaaataa gaacattcaa tccgtgtttg
    11401 attaagtcat gtgcgaattg ggcttggata tcagaaaagc caatgattaa atctggtttt
    11461 aggtttttaa ttttttgaat acttccggaa ataaaagcag aaaccactgg tttttctttt
    11521 ttagccttct caggtctaac tgtgtaagca gtaattccga caatcctatc ttcttctcca
    11581 agaaggtaaa gaagttcagt tgtttcttct gttagacaaa taattctttc gggagaatgc
    11641 atgaattaga atccattcga ctcatattga tagagtttaa ttccgacaga agatgggtca
    11701 gcacaaccgc ttacagtaga ggctgagact aaatttccat gaccttggta ggctccttgg
    11761 tttggggtca ggagacaatc ttcattagcc tcacaaagcc cattatcatt tccaactcca
    11821 tcattgagta attcaactgc atttcgaaga attgtagaag agcaagtgct tccattaaag
    11881 ctcgcaccaa ttacactatt acaagcagct tggctagaag cagaccaaat atgcgcaaac
    11941 atagtattag ccgcaggaca accacttcca cctcctgtga tatttgcatt gcgcaaagtt
    12001 gtatctgttg atttcaaact aaaatcccaa actgagcaag aaccgctgga acaatttcct
    12061 tgggcggatg caagtggaaa cgatgcagca ggcatcatag ctgctctaaa aaaattagaa
    12121 aaattaagcc aatctaaact tgcactatag gtagcagaac cagatgaaaa tgatgcgtta
    12181 gttgaatcag aaattgaaga tgtgggaact cctataaaag tagaagcact taccgaccca
    12241 gaattggaag tagtgataga atctgagggg ctagaaagag gacaccctcc agagctaata
    12301 cctccgccta catatccagc tgccatttga caattagcag tattattaga taaacgaatc
    12361 attcctctac cataaaaact tccccctcct gataagttaa acccatattg gttggaaata
    12421 caagaaatat tctccattgt aggatttgaa aaaggaccac ctgatacacc agctgttcca
    12481 ttattgacag aagcaaggtt taaaaagaac gaactagcac tcgaaggatt aattccatct
    12541 cctgtattat ttataagagt agcattcata aaggagttat cattcgcgcc actaaaggaa
    12601 actccatttg cggcatttcc ggcggaaata attccattgt aaatattacc agttgaagcg
    12661 gatgaattta caccagttga ttggttattt gctagaatga ttgtattgaa gaaattatta
    12721 aatgagtttg ctaaataaat tgctccgttc gttgtccctg ttcccatatt tgccacaacg
    12781 atatcttcaa aataattatt cgatgctgag ttcaaataga taccaacaca attaccagcg
    12841 gcataacaat tctgtgcttt aaaatttttt aatacagaaa attttccacc catctgaatg
    12901 gaatagctat aaaactttgt ttcgagtgtt ccttcgatcc aattaaaatt tccactcgtt
    12961 aaaatggcag gtgttccagt attgcttgtt ccgattgtat aagtttgccc gggtttaaat
    13021 acgatagaca ccttatcagt cgtagaagta ttgatttgtc ctgctgttac attcgcagag
    13081 taaagataaa ccgttccact tataaaacca ctagttggac ttgttgtatt catcgcatta
    13141 gtccaaaaag taagtggatt actaactgca tgagtcgttg ttccatcttt aattgtaatt
    13201 gtgagtgcct gccaagtatt tgtagtccaa tcaatgagtg ttgataaata ttttcctttt
    13261 tttaaagcac tcgaataaaa cgttactcca ccactagaat ttgttttaca aacccaattc
    13321 aatgccccta aattatcaga tgctgtcaag ttggtgcaac tagttttaga tggaatatct
    13381 agctttctaa tttcacccgc atgaacacaa gttctatatc ctcctgtgtt ggaactagaa
    13441 caagcactgt ctgtttgtga aaaaatatcc tttgtaaaat ctcgattgat ataatcattc
    13501 cagtttcttc cgttcgtaac atatacaggc gataccgaga tcacgttaca tgtgatattt
    13561 acattattca catcggaagt agcagtgcct gacgcatttg aaacagtgca aatagaacca
    13621 actggatttg tttttacaga aattgaatac gcggtgttac gcgctacttt aaattgataa
    13681 gtagttgcag gcggatattg catatctgat cctaaattat tttgaagaac tatcgttcct
    13741 gaattaaagc ctgttatagt tcctgaaata gtaatatctc cgaagaatgc attaaaaaac
    13801 aagccaatgt taaaaaagct cggtttacta aaatcaaaag gagatttctt aacttgaaga
    13861 catccagtca ctaatataag tattaaaatt aaaattgatc gcatatcatt acccgaggtt
    13921 attccattaa ataaattatc tctcatttag aataacagct gaaagtcatc catttaccgg
    13981 attacttctt attaaccacg aaattattga taataaaaaa atgaaacctt tttttatttt
    14041 ataatttacc ggattacttc ttattaacca cgaaattatt gtttatttca atgaaaactc
    14101 tcttttattt cgaccagatt tctcaatagc ggtatattat tgttatacga tctttttttc
    14161 tattgattag aactttttgt ttataaaaca atcctccgta taaactacta gtttctctat
    14221 acaaaatgac tcttacaatt gaaattaaaa ggtgatttcc ctaaaacaaa tcctaaacta
    14281 aatcaatcac cattcagatt ttttgagata ggaaattact attgagattg aatttaaaac
    14341 actagacaaa tcattctccc tacaagattc tcttcttaca ccatttcaag gttttatttt
    14401 tctaaataaa agtcgttata cgagaaagga aattaactat atgaagaaat tactgattac
    14461 gaaagaagat ataataaaaa ttgagaatga attgttttca gctcagttat caagtaacgt
    14521 tgaagtttta gataaactat tattcgattc tttaattgct gttaccccaa atggacaaat
    14581 tgtcacgaaa gaaatggatt taagctccca cagagacaag actatgatga ttgaagatgc
    14641 ttctacacat atagatgaaa ttcaaatcat agaggatatt gcaataactg ttgttacaat
    14701 gaaagcaaaa gggaagatga tgggatctcc catggaaggt cattttaaat atctaagaac
    14761 ttggaaactt ttagatggac aactaaaaat aattgcagct tcgattatga tcttaccgga
    14821 ataatacaaa taccctatat actttttttt caaacagaaa aaatatatta cacatatcgc
    14881 atttaatttc acataacaat ctattctttt tttgaagaaa gaggatagaa tataaattta
    14941 aaatcgcttt aagtataatt tattaatttg aaaattatac ttaaaaagag aatataaata
    15001 ttgattcaca tataagctag gtcttattct tttttaaaaa tatccgataa gaaccaagga
    15061 ggaaattttc atgatttcaa aaaagttttt aataagtttc attttaatgc ttagcctttc
    15121 tatctatgca aatacagatg aggtattact ctatgctgcg gaaaaggggg acttatctca
    15181 agtcaaaaca gccctcaact gggatgggga tgtaaatgca agtgatcgta atggtgccac
    15241 tgccttgatt aaagcttgcg aaaatggtca taaagcaatt gttgagctac ttctaaaaaa
    15301 gggagctgaa gtaaatgcca gagataaaac cggtgcatct gctatactat atgcaagcat
    15361 tgttggtgat aaaaaaattg tagaactact tataaaatat ggtgctgatg taaatattaa
    15421 ggatgttggc ggcaaatctc cgttatccga agctactcaa tttcaaaaca gagaagtcat
    15481 cgaattactc aaaaccgcag gcgcaaagcc atagaaaaaa acttgtatta atcctataaa
    15541 gcaaaaatct gactttaaga tttaaggagt ttctatgtta ttagttgcta aaagattaga
    15601 tgttgtggag ccttccccta ctcttgctat tactgcaagg gcaaatgctt taaaagcaga
    15661 aggaaaagat gttgttggtt ttggagcagg tgagcctgac ttcgataccc ctgagcatat
    15721 aaaaaatgca gccaaacttg caatggataa gggacaaacc aaatatactc ctgtttctgg
    15781 aaccgttagt cttaaagatg caattatcaa taaatttaaa aaagataaca acttagtata
    15841 tgatcgtaaa aatattatag ttggagttgg tggtaaacaa gtattatata atttttttat
    15901 ggcaacaatg aatccaggag atgaagtaat tattccagct ccttattggg ttagttatgc
    15961 tgatatagtc agactagcag agggaactcc tgttatcgtt caaacaactc ctgaaaataa
    16021 ttttcaaatc actcctgctc aattagaaaa agcaattact cccaaaacaa aggtttttat
    16081 tttcaactcc ccttccaatc ctactggagc agcttactct aaaaaagatg tggaagcgtt
    16141 atgtgaagtt atactcaagc ataacatcat ggtcatttca gatgatatct atgagaaagt
    16201 tatctatgat ggactcgagt ttgtaaatcc agcaatgctt tcacctgaaa tgaaagaaag
    16261 aacgtttgta gtcaatggag tttcgaaagc ttattcaatg accggttggc gcattggtta
    16321 tggagcaggt catccagaga ttataaaaaa tatggataca atgcaaggac agtcaacttc
    16381 aaatcctaca tctatcgcac aagcagcagc ggaagaggct cttaaagcag accaagcatg
    16441 tattactgaa atggtaaaag cattcgatga tagacgcaaa aaaattgtca aagctttaaa
    16501 tgatattcca ggggtaaatt gccgtatgcc acaaggtgct ttttatgtat tcccttatat
    16561 cactggcgta tatgaattac ctggattcca agcacttttg aaagcaaatc caaatgtatc
    16621 taaaagcaaa ttattttgcg acgtactact tgagaaatac gaagtggctg tagttccagg
    16681 cattgctttc ggggatgata gtgcaatccg cctttcttat gctcttggtc aagccaatat
    16741 tgacaagggt gtagcgcgta ttgctaaaat gatttctgat ttaaaataaa aataacttaa
    16801 ataactcctt tgtaataact acaagggagt tattgaaacc caccacattt tcttctaagg
    16861 taattccgtt tttaatcttc tcaggttttt agttctaatt tcaaagattt ttataattcc
    16921 atttataatc gactcaagaa caaacgggtc acaatgattc aaaaactcct ctgcagaaat
    16981 tttattatca tagaatcctt ttgatatttc aaataactta tctatttcag gaaacccaat
    17041 cgcatttact aaaaaagcag tgatcggaga gttttttaaa tcataggctg tatctttatt
    17101 tcttcctttt gtaagaatta aaagagcctc ttcttcaatt ggattctgtg gcacataagg
    17161 attttctacc ggaggtaaaa aataatcgag cgttgggatc actattccat ttacaggaat
    17221 ttcacctatt aatgtggaaa gtggaatatt tcttcctatt ctaaaagaca atgcttctaa
    17281 aatcccaact gtcagaattt tactttctaa ataaattcta gataattcta aattttttcg
    17341 cgtatcctta aaaagcttct ctagtttttc atctgaaggc tcatccatat attgatgaaa
    17401 aataaactct ggttgaaggg tttttagaaa gttaaccatt ttgagcatag atgttctata
    17461 tccaacaata gtataagatg tatgtgtcct gagatcatga tttgtctctg gcattaaatt
    17521 ccaggtatta tctaaaaact taacagaatt cggatttcca aaattttcta catctctatt
    17581 tgccattctc aatgcttttt ttaaggtttc atttatacga tcttctgacc aattcaaaga
    17641 ataataacta ttaaccgctt ctaggcgaac ctttaagata tcgtatggag ttttattatc
    17701 cactaatctt ctaaatggaa tcgtcgcctc aatacaagca gctatttctg ctaaggctcc
    17761 catctccaaa taaggctcta gacattgcat agcaatcatt gcactcaaga attcattttg
    17821 accagcataa ggagataata tatcgcctaa cttaaatcca aagattaaaa gtaaaagttg
    17881 aaaggtttta tttttaagca gttctggttt attgataata tataatttat tgtcatcttc
    17941 tcgaacaaaa ggataaagat atccgcttaa gttgatattt attcctgaat ctacctggat
    18001 ataaatcata tcatgaaaga gcgcggctaa catttcaaca ggatccttat ctcctcccac
    18061 ttcaaagata tgttcaggag tgtgaaaata tctccaagtg cctgtcatgc tatgaatgat
    18121 taatttggaa atgtaattga tatcctcatc attctctaca tctatttttc cgcctaattc
    18181 tttaatcgaa tcgattaacc gtattttgca tcgctctaaa tctttttcca tttcttatcc
    18241 gcttttgttt tattatggtt taaacttttt tataatgatg gatatttatc ataaaaatcc
    18301 atcattataa attggtactt tttagttcac cttcacattc accccaccgg ctggttcttt
    18361 ataattgatt tcaaataaat cgtagtcgga ttttctaaaa ccagttgcat aagcaactga
    18421 aatacctaaa taatatttac caccagattt cattgcagct aacgcatatt tagccatctg
    18481 ttcatttgtc acggaaaatg gatgcacttt acctcttgtc tcatctagat aaagaccttc
    18541 gtaagttcct ataatagttc cttgataatc gagttgtaaa actttacctg taacagaaaa
    18601 atttcttttt cctcctgatt tttgaacagc tagcttttct ccttgttctg gagctggaga
    18661 agattcttgc tttgctgctg ttaatatttc catatcagaa gaaaggctaa ttggtgttat
    18721 ccgatgaata ttatatccca aaagtaaatc ttgattcaga ttcttgctga tgaaattaat
    18781 caattcaggc ttatccattc ttacactaaa attcgttttt gttttttgag gagcaaaaca
    18841 ctcatctttt gcagaatcac atttttcttc cttgtcaaac tttgtaactt ccatgactcc
    18901 ctcaaaggat tcaaatagaa ttccacgact ttcgaattgt atgatttttg cgacaacgga
    18961 accttctgcg taagtcccta gtgcatatac agaagaagga aggatggaag tgatacataa
    19021 aatcattcca cctaataaat tttttttata catctttatc tccaatgatt cacccaaaaa
    19081 atttttgagt attaatttga aataatatct aaaatttact attcaagaat cttattccaa
    19141 gtaaagcaaa gaatattcaa aataggtttg caagaaaatt ttgttaaggc ttgcatttaa
    19201 aaattgtttt atttaaacgg ttagtcagaa tgaaaatctc acgtgataca aagttttttg
    19261 gaattcttgg attccccctc ggtcatacac tttcccctgt tattcacaac ggcttatttg
    19321 aagaatacaa cttcaatggc ctctacatcg tcttcgaaaa agaaaaaccc tctcgaaatc
    19381 ttttttttgg aaaggactcc atcccgctag aaggactatc tgtaactatt ccacataaag
    19441 aatgggcttt caaacaagct gatcacagag atgaagcctc aactcatatg caagcttcta
    19501 atacgctcgt aagagtccaa tcaaaactga atgcttataa cacagacggt tatggggcaa
    19561 ttcgatccat tgaagaatat aaaagaaact tttttgaaac tggtaaagga gatattctta
    19621 ttctaggtag cggtgggagt gcaaaaggaa tatcttactc gttggttaaa cgcggattat
    19681 ccgctcaaaa aatcgtagtt gctgcacgca atgaaaaaac gggaaaagcc ctcgtgcaaa
    19741 aattaaactc aatgcaaaag gagattagtg aatatgcaac actcgaatcc atccaaaaag
    19801 aatcgaaaag atattctcta atcatcaaca caacacctgt tggcatgaaa ggtaaaaaag
    19861 attcccaaat tttatcagaa gattttttta taaaatcaca ttctcttttt gatattgtct
    19921 ataaccctct cgagactcct ctcgtaaaac tggcaaaaaa gaaaaaagta aaaattattc
    19981 ccggttatga aatgctcctg tatcaagcaa tggaacaatt tcgtctcttt acaggtattc
    20041 gtgtggataa caagcgtata tctcttgtca gaaaatggtt attaaaaaat ttaaaataaa
    20101 gaatttataa attagataat ccctaatcaa gttttaaatt ccttatagat tcatttattc
    20161 taaatactgt ttctattgat tctgtttttt ttgagtatca ttgaaactag ttattttttt
    20221 tatcattcaa gaataaaatt caaaattacc tgcacaaacc attcgatttt tttctcttct
    20281 taaattagag gtattcctat gtttaaaaaa ttccatgaac ccattacgct ttctaactca
    20341 aaattggctt tccccagaaa tgatttcaat ctttccgaat ttagttctac catcttaatt
    20401 cccgaacggt atcttgaact ctttttctca aaagcgaaaa aacatactgg agttcgcaac
    20461 tatatcgcat acctcctttc taaataccaa ctctttattg caaatggtct aatccccggt
    20521 tgctcaaata tcactactaa atatcaggat aaaggtcaaa acctacagaa agtctcattc
    20581 cgacctaagc cagaagattg ggcagaatta aaactctatc gtgtaagctt cggtatgtct
    20641 atctctgctt ttttggtcta cctactaata gctgactctg ttgaccttgt agagtctctc
    20701 tcggactacc tagggggtgt cggaatttcc attccaccgc attttgactt gtgcgccaaa
    20761 gtttatttaa cccaaaaaag aactaactat acgatttttt tccaataccg aaaagagcgg
    20821 aaagtgcgag acaactaaaa aatccagatt tttccaaaaa cgcggctaat caaaataaac
    20881 cacacaaacc aaataaggcg aagctatgct ctaagacaac aaacagctta atctgatagg
    20941 agattgagtt aaatggaaag gtccaattaa aatagagaat ttaaatttta tacaccaaaa
    21001 agttgcacaa aagtcaaatc attctactta aaaataggaa aaacttgaat ggcaagcaag
    21061 tggatagaaa ctaaatggtg tcatacgata gcgaaaatgc tcatattttt atcttgccat
    21121 ccaactcatg caaaactaga ttgataggaa atatcaataa attaggacag attatcaatt
    21181 ttgaaagact gggttaaaga aagttatagt attcgtaaat gggcagggga gctaggaaaa
    21241 gaaaaagcag gcgagacagt atttgtatgt ggttgggctt ttcgatttag agaccaaggg
    21301 ggagtgatct tcatcgattt gagagaccga accggaatta ttcaagttgt tgcgcgcaaa
    21361 gaaaatatac cagaagattt taaattagcg gagcatgttc gaagtgagta tgtgctagca
    21421 gtaaaaggta aattaaacaa ccgcgacgct gaatctatca atcctaagat acctaatggt
    21481 aaaattgaag taattataga aaaattagaa attctaaata catctaagac acctcctttt
    21541 gctttggatg agtttgatga atccggagaa gacattcgtt taaaatatcg ttatttagaa
    21601 tttagaaaag atgaactcaa aaacaaaatg atacttcgtc ataagtttat ctttgccatt
    21661 cgaaactatt taaatgaaaa aggatttctc gaaattgaaa cacctatctt gaacaaatcc
    21721 actcctgaag gagcaaggga cttcttagtt ccatcacgtt tacaccaagg cttcttctat
    21781 gcacttccac aatccccaca aatcttcaaa cagattttaa tggtgggtgg aatggaacgt
    21841 tatttccaaa tagtaaaatg ctttagagat gaagacttgc gtgcagatag acagccagaa
    21901 tttactcagc tcgatatgga attttcttat atagatcaac cgaatatcat ttccgaaatg
    21961 gaagcgatgg tttgtaaagt atttaaagaa tcgtttaacg ttgatttacc aagtaaattt
    22021 ccacagatga gttatgcaga agcaatggat tcttatggaa gtgataaacc ggatttacgt
    22081 tttggaatga aactttataa cgtatcggat atcgtcaaaa attgtgactt ccaggttttc
    22141 aaaggagcga ttgaggcagg cggtattgtc aaagcaattt gtgtcaaagg cggatcgatc
    22201 atctccagaa aagaaattga agacctaacg cattggcttt ctaaagattt tagagcaaag
    22261 ggacttgcct acatgaaaca tggcaaagac ggactcgaat ctacaatcac aaaacgattt
    22321 acaccagaag aactttctgg aatcgcaaaa gcggttggat cttctgaggg agatatggtt
    22381 ttctttggcg cagataaaat ttctattgtc aatgcaagcc ttggagctct aagactcaag
    22441 atggctgagc gtttcgaaaa attagatcca aatcaatatc atatttcttg ggtagttgat
    22501 ttcccaatgt ttgatgtaga tgatacaacc aaagaactca gtgcaattca ccatccattc
    22561 acagctcctt tcgatgaaga cttcaatata aatgattcta tcgaagaact aaaatctaaa
    22621 gcatccaaaa tgcgatcaaa ggcatacgat ttagtattaa atggaaatga aattggagga
    22681 ggaagcattc gtattcacaa agaagagatg caagaaaaag tatttcaact tcttggaatt
    22741 tcaaaagaag atgcagaaaa gaaattcgga tttttactag aagcactacg ttttggtgca
    22801 cctccacatg gagggatcgc tctaggtgta gatcgtatca tgatgcttat gtctggaagt
    22861 aaatccattc gagatgtgat tgcatttcca aaaacacaaa aaggaacttg tatgatgagc
    22921 gattgcccaa ctcccgttga tgacaagcaa ttagaagagc taaaaatcag agtggtaaaa
    22981 gtttaatgcc aaaagaaaaa tcacagccta tccaagaaaa atttgaattc aataacgaag
    23041 ccttatatca aaaagtctgt ggaatcaacg actcaagact taaggatttg gaagcatatt
    23101 taggaatcac aattattccg cgtggatatt ctctgattgt cagtgggcag aaagacaaag
    23161 tagacatcgc aatggagttt tttcgtaacc tagttgataa ttacaaaaaa aggccagaca
    23221 aagaagactt tgatagtttc gatttaaact acctaattcg tcaggaaaat caccaagatg
    23281 gatggactcc caacgaaaaa attctaacta ctttcaaagg taaacaaatc tttcctcgca
    23341 ctaaaaatca ggagagattt gtaaatagcc taatgaaaaa cttaatcacc tttggaatag
    23401 gaccagcggg aacaggaaaa acttttctct ccattgctgt cgcctgtcgt cttttacaag
    23461 caggtgagat agatcgcttg gttttgacca gaccagccgt agaagcgggt gagtcattag
    23521 gatttctccc aggagatctt acacaaaaag tagatcctta tttaagacca atttacgatg
    23581 ctttatacga atgtattgga tttgaaagag tgcaagtttt aatttcactg aataaaatag
    23641 aaattgctcc tatcgcattt atgagaggaa ggacattaaa caattctttt atcattttag
    23701 atgaagcaca aaactgcaca acttcacaac tgaaaatgtt tctgactcgt attggcaaaa
    23761 actctcgtat gagcctttct ggagatgtaa cccaaattga tttagacaga ggaagatctg
    23821 ggctagagaa agtcttatct atattaaaca acactcccca aattggagtc atgcatttag
    23881 agaaagaaga tataacaaga catccgcttg tctcagtcat tgtagataaa ttcgaaggta
    23941 tataaaattg aattttctag aacgtgtaga atctttcatg gcaaatacaa ctgacgtact
    24001 tacaaaagtc cgtcctattt attttgttag acagcttcag ttttatctcg ttctagttac
    24061 attgacaatg gtaacatact ttcttgcttc tccttattta ggacaagaaa agacagatat
    24121 ggacgatgaa aattcctatg ccgttgggaa tgtttctccg gagacaatca tctcaaacaa
    24181 agaaattatc tatgatgatt tagataaaac aaaattaaaa agagcggaag cctataaatc
    24241 cgcttctctt attttcgaaa gagactattc gattttaatt aaccaaatac aaaagtatat
    24301 tgatgaagac tttgaaaatt taaaagttat tgcatcagaa ggcagtaata ataaaaacat
    24361 cggttttcta atctataaaa atatccgctg gaaatttcgc tctaaagagg atttagaatt
    24421 tttattacag tatccaagaa aggaaaaatt aagagaacta gttcaaaaag gaacgaattt
    24481 agtgttctct tcttattgta tattaaagga aatttctcct gaagtaatga gttctcttgg
    24541 ttcaggtgct catataatta ataagggagc caaggaaaat acttccacgt tagactcttc
    24601 gcatgtattt cctagaactg ctatttatca aaatcaaaaa gtccaagaac aactttcaaa
    24661 actcttagat gagaagttaa caggcattga taccaatgct ttaacaatca caaagagaat
    24721 gattctatct agttatattt attccttccc tgcttgcacc tttaaaccgg aagaaacgga
    24781 acaagaaaaa caaagagaag ttgcaaaggt aagcatcgta aaaagtaaaa tagcaaaaaa
    24841 tgaagtaatc gtaaaaaaag gagaacttgt cactcctgaa attcgtgcta aattagagtt
    24901 actcaatgaa cacaattcaa gagccaatgt aaattcaatc ctatccatcc taattattca
    24961 aatcatttta gtaagcatca tcatcgtatt tttaatcaag tatgatccca aaagactcaa
    25021 tgatgtttcg agtaatgtaa tcctattttc aaccatatgg ctcttaacgt cttacacctt
    25081 tatcatttct aaatttttct atcatccaga caataacttt gaatctattt attactttgg
    25141 gatttttatt cctattggaa tgatttgtat gctgattgca tttatttttg atgaacagct
    25201 ttctattgca cttgggtttt atttgtcttt cttcattttc ctttgctctc aaaataatgc
    25261 tacatcattt atcattgcat tttccacgac agttgtcgca tccatgtatg gtagtaagat
    25321 tcgaaagaga attgatttta taaaatcagg aatttatatt gcaggtgttc aaatactcgt
    25381 tgctacaagt ggttatttga tagattcaag acaatattgg gttgctacat ccactggatc
    25441 tctactttct gatattgttc gttccaatat ttttaaaatc tatgttgctt gtattgtaaa
    25501 cggatttgct tgtgctctca tgactcagtt tttacttccc gtatatgaat acatttttaa
    25561 tataccaact cgattcaaat tacaggaatt agcagataca ggacatcctc tattacaagc
    25621 gttactcacc aaggctcctt cgacttatac tcatacattt atggttgctg cactttcaga
    25681 aagagctgcc caaaatctag ggctcgactg gctattagtc agagttggag tttattttca
    25741 tgatatagga aaaatcccaa atgctggttt ttttgtagag aaccaacatt taattccaaa
    25801 aaaagaaaac attgataaaa acaatcccgg acttgctgcc aaaattgtaa tcgatcatgt
    25861 catcgatgga attgaaatgg ccaaaaaagc aagacttcct agagaggtga tcagttttat
    25921 tccagaacat catggaacaa gcacaatggc tttcttctat cataaagcgt tagccgattt
    25981 atcacccaat caaagaaaga aaattcataa gaaagatttt atgtatccgg gaccaaagcc
    26041 acaaagaaaa gaaactgcca ttgttatgat tgcagattct gtcgaagccg caagtcgttc
    26101 cctagaggaa attactccac aagccttaga tgaactgatt caaaaaataa tcaatatcaa
    26161 gcttgctgaa aaccaacttg atgagagcgg actcacactt ggagatttaa gtattataaa
    26221 atcctcattt aaagaaatct tactatctag tttacaccaa agacctaagt atcccaactc
    26281 agaagataca aaaaaactag aaaacaaaga agacctaaaa aataaacctg tccctaaaac
    26341 accgtctgcc aaaaagataa gtagaaccaa aacaaaatag aatatcgaga ataattatca
    26401 attttctcct ttcgaaacac tcaataactt cttttttttg caaaataatt tcttgacagg
    26461 aatttaaaaa tccaatatat tcttccactc tgaaagtatt aaaggataat tctatgaaat
    26521 ttatttacct aataatgatt agcctactct tttcttattg tagtagttct cccacaaagg
    26581 atgaaaaacc aaataatgtg gaaccaacag ccaatgttca aaaggatgac ccaaaacaga
    26641 aatttctcga tctagcctat gccacatcta aaaatattaa agatctctcg gataaaagta
    26701 tagcctctaa gacaaaagca ttgctaaacg aagcttatgt tctcttgaag cttaaaaaaa
    26761 atgaagagtc tatcgcaaaa ttcgaagaag cttttgagat tggaactgac gcagaagcat
    26821 attataaata tggaaatgcc ctttccaata caatgaaact agaagaatca atcaaagcat
    26881 atgatatcgc tttagagtta ggctacgaaa aagaatacaa tgcaatctac aataaaggtt
    26941 gttcttacag ccttttaaaa aatgaagaag aagcttttaa aaatattcta cttgcagtcg
    27001 atagaggtta cacggcaatt ccctacatgg aaaaagatcc tgacctaagc tatatccgat
    27061 ctctacctga gtggaaatca aaatacaaag agattaaaaa acatgcaaaa gaacgagagg
    27121 aggttgttaa atccaaaggc taattgcgat taacctcttt ttaaattaat ttcggtgatt
    27181 tccgcggggg ctccaatacg aattggtggt ccccagtatc ctgttccact gctcacataa
    27241 agccatatat ctccatgttt ataaagccct gctacaaatt tttgaaaaag atgtattaat
    27301 agatttccag ggaaatactg acctccatga gtatgacctg acaactgtaa atcaaaacca
    27361 acgatagccg cttcaaatac agaattcggt tgatgagcaa gtaaaagctt aaaatccgtt
    27421 ttttcttttg acagaattgc tttttctgga tcagaagcat gggaaggtaa aatagtatga
    27481 gctttataat cagtaactcc ccctactaac aattttgctt caccggaatt cacaatatgg
    27541 ttttgattca ataaaacatg gatacccaaa tttctaatct caggtaacca agataaaacg
    27601 cctgagtagt attcatgatt tcccgttaca aaaaaagatc catattttga ttttagattt
    27661 tttaatggtt ctagatgatg cttcaactta tatacagagc catccactaa atctcccgtg
    27721 atagcaatta tatctggttg taatacattg atgcgtctaa cgacttcctc taaaaaatcc
    27781 tttttgattg taggaccaat atgaatatct gatatttgca caattttaaa attttctaaa
    27841 tcaggatgta aatcatttat tggtatatcg acccttttga catcaacttt gtggactgct
    27901 ttgtaaactc cataacctgt aaggctaatg gaaattaacc ccaaagaagg tttgattaga
    27961 tgcaagaaaa aatcttttct atttatgcta gactccattc cttgatctgt gattatttct
    28021 gtcctaaaaa atgcaaatac agcttgaaat ccatttacaa tgctaattga caaatcatga
    28081 atgagcacta aactaaatag aattgttaaa aatccaagac tagtaaatgc gataaaagaa
    28141 aaagatttct gccattctga ctcttcaaga aataaactaa aaaaataaga tactggcgta
    28201 agcaaaataa gaaagaagat tgctacccaa aaaaggattc taagtgatcc agaaatggaa
    28261 gtcccttcca aaaatcgact tcctacataa taatacataa gaccaattaa gattgtaatt
    28321 atagtgataa aaataaaaaa acgctgcaag taaaactcct actataaata ctttgggaga
    28381 cgaaagagga gtcaatctgt taaatgaact cctttaaatt attttaataa atatctaatc
    28441 taatcttact cataaaatag attacttaca atttataatt tcataaataa aggtttaatt
    28501 gttaagttac aaactgagga accaactaag gctcaattct agataacaaa aaagcgatgg
    28561 gtggcaacca ctactccttc cctgttaatt ttacgtagga gtatattcat tttttaatac
    28621 caatataaaa agccatcatt taactgaaaa gcttatagtg gtaaaatgaa atactattta
    28681 tcaaaataaa aacttgccgt tggatagatt ttatatattc tgaacgtagt aacttctatg
    28741 aagcaaatat taatagttga tgattctgaa actagcatat tatatttaaa gatggtttta
    28801 aaagattata atgtagtatc tgcaatcgat gggaatgaga tgtgggagcg gctaagagaa
    28861 agtatcccct ctttaatttt aatggatgta atgcttcccg atgtaaatgg atatgaacta
    28921 gtcaaacaat tatctactac atcccaatac aaagatatac caatcatttt tcaaacagct
    28981 aaaagttcaa gtgatgatct agaaaaaggt tttgaatcag gtgcaattga ttatatacga
    29041 aagccagtag ataaaaaaga actaagagcc agaattaaat ctgttttaaa aattaaagaa
    29101 ctcgaacatc aactcttcat aaaatcaaca acagattatt taacagaaat ttataataga
    29161 agatattttt ttgaaatagc cttaaaagaa cttgagtcta ctatcgcatc aaacaaatgt
    29221 ttaagtatca ttctgctcga cattgatttt tttaaaaaga ttaatgatag ttttgggcac
    29281 gatgcaggag attttgtttt aaaaaaattt gctgatatca taaaaaatca tattcgtccc
    29341 aaagatgttt ttgctcgcta tggtggtgaa gaatttattc tcctcttccc tgaatctgat
    29401 aaaaatacat ctatctcaat tctggaaaaa atccaaaaga taattcgaga aaatagcttc
    29461 ttttttcaag ataaagaaat ttcatttaca ttcagttgtg ggatcttctc tttagaagaa
    29521 actgttcaaa atgtaacact cgactcccta atacaaatcg cagatactag actctatgca
    29581 gccaagcact taggaagaaa tcgaattatt tcttctgatt gataagaatc ccccttaaaa
    29641 acttacgtta gatacatccg agactaaaag cattttctaa tttagtattc ctaaatacta
    29701 tcatatttac ttttctagat ttaggaatta gttagaatat tttgattaaa tctcttttct
    29761 ctttttttct agaagctcta acagattgta atttggaaca tatccctctt tatcaagcga
    29821 gtattctgat ttaattcttt tcaggtaata cattttaatt ttacctttat tttttgcgtc
    29881 aacttcccca cgaaattcac attcaaatac atcctttaca cattcatatg tattgtgaga
    29941 aatatttata tttccaggag tgccagaaac ttccattcga cttgcaatat tgacagtgtc
    30001 tccccaaacg tcatacgcaa actttttctc tcccacaact ccagccatca ctggacctgt
    30061 gtggattccg attctaagtt cccaaaaaga atcactcatt ttttctttta cttgtttcat
    30121 atgattcata aacgattgaa tttcgagagc agcaagacat gcttcatatg ggtgtgtaac
    30181 accttttcgg ggaattccac cggcgcacat ataactatcc ccgattgtct ttaatttttc
    30241 tagataaaat ctttctattg tcttatcaaa ctgggtaaag tatccatcca attccttgat
    30301 caacttttgt ggagacatgt cctctactat ccgtgtaaaa tctttaaaat ctgtaaacat
    30361 aatcgttaca tgatcaaaat gaacgggagt aacttctcct ttttccttta gctcttctgc
    30421 aacctctgca ggtagaatat tcagcaaaag ctgatctgat ttttgtagag cagaagctaa
    30481 atccttagtt ctttctttta ctcttacttc taaactatct ctttcttttt gtaattcatc
    30541 gtatgcttgt gcatttgcaa gggatatacc agctaacgat gcatacgctc caagaagtgc
    30601 ctcatctttt tcagtaaagt gatctgtata ctttttattt agaacctgaa ttactccaat
    30661 gatttctttg gtcgaactat agactggcat acaaagaatc gatttagtgc gatatccact
    30721 ttttaagtca ttttcgggag aaaatctaga atccgaataa gcatcgcgga tattcactgt
    30781 ttgcctcgat tcggcaacat atcctgcaat tcctgcccca tactcgattc gaatctcttc
    30841 cttactaccg agtgccactc ttgaaaatag ttcatgtcgt tcattatcaa caagaaataa
    30901 agtcgcacga tctgcattca tcacataggt tacatcttgt atgattaaat cgaggagtga
    30961 gtctaaactc atttctgcca tgatggatct tgcggaatca actaagacat ttaaatttct
    31021 cgcctgttga ctaatattct ctagttctac tgcatctaga taaaactttc catcttctgc
    31081 tttgtaaaga ccgggtatta catcggtctt cctttttccg tattctgaac cggaaataat
    31141 tacttcatat tttaactctt ccacacaaca atatataata gctagttgag tttagtcaaa
    31201 actaaatttc ggaagtgagc taactttggt aaaaatcccg caaatctata tcttgaaatt
    31261 caaaaaaagt tcttaagagc attgattctt ttactctatt tctcttattt catattctgc
    31321 atttttaata atcagtcgtc cagttgcatg agaacctgcg tatctatata aagatgcttc
    31381 cctttcgtct tctgaaaaag taacaattaa tttatatcta cctaaaaaaa ctttttctct
    31441 ttctgataca gtatactttc tagttttact cttatcaaat ttaccgtata gacctcggct
    31501 acgataaccg tcatttttcc attgatacca atttcctact acttctgtat tttctacaag
    31561 atagggagaa taaactttat ttgcaggaag agtatcaaag aagtagcaat cgttctcctg
    31621 acgattcgag taaatcaaaa ctgttccaga aaggatcgca actttctgaa attctttttg
    31681 tgaaagagga atttcaccat ccttcctttc tacagtaata caaagataag cttgcccaat
    31741 cggaattgca tttttagtaa ttgaagagca atgtgtgatt atgagtaaaa aaatagcaat
    31801 atataattta atcattccaa ccattttcct tgtcagttgc tcctacgacc atgtaaatat
    31861 tttttctact caattttttg aatcagttcc agtttcaaaa aaaatagata gaactgtttg
    31921 cataaatccg gttgaaataa aaaataaaaa tataatagag tataggaaag agtctagaac
    31981 aaatcaattg cacatcgggt taacagaatc tgaatttcaa gcagagaaaa aagaatttgt
    32041 taaaatattt tctgaaaatt ttctatctta cctaaaatca aaaaatattt tttcgagtat
    32101 agacttatgt gaaagagaaa aaagagaata ccagttaagt attacactcc aagaatacac
    32161 tattacaact cattttcctt tccaccccgt tttagcaggt gtccttttag ttcctatgtt
    32221 ttttattaca aaagaatcaa acctttctat tcagtttgat tttaaaaaat cagaaactac
    32281 aatcgcttct gttacaagaa aagagaatcg agaaagtagt atatttacta tcaattatta
    32341 tcctcctaaa accttttcct tagatggata tttaaaccgt caatacaaag gagacaaaat
    32401 ctatattgat tatatactag atgatgcaat cgacgagctt tctacaaaaa tttattctaa
    32461 ttaacatgag tttgatgtaa caaaaaattt aaaagattac cacagatgaa cactgatgga
    32521 cacggataaa ataataatta ttaaaaacat ccgcgtttat ctgttttcat ccgtgtctat
    32581 ccgtggttaa atattcttac atcgaactga tgttaaattt tcgctttatt ctactcttta
    32641 taaaaatgca aaaactagat cttgcaacga acgaaacgga ttatctactg gcttactctc
    32701 attagccgct acaaactcat aatcaagtgg attcataaac aaagttcgta gacgaaattc
    32761 cattgtaaat cctggccaaa gagttgtatt ctttcctgtt tttgtcacat accaactatt
    32821 acaattttct gcccagactg atttctgcag tctcctttgt atctcttcat tgtaatgatc
    32881 ttgcacttct ttttttacat tcatatattt taaatttctt ttctttaaat ttagaataga
    32941 atgcacaatg taatttgcct gtgattcaat cataaaaatc atagaactat gaccgagtcc
    33001 agtattgggt cctgtcatta aaaataaatt tggaaatccg gaaacggtag ttcctagata
    33061 agtttccgct ccagactttt tccaaatctc attcagattt tgcctattga gtcctattac
    33121 ttcaaatgga gctaacgctt ctgctccttg aaaacctgtt gcagcaatga ttgcatctac
    33181 aaaatattct tttccttctt tcgataaaat tgaattctct cggacttcta aaatcccaga
    33241 aacatctaca tttacatttt ctctctcaag cgtaggatag tagtcgtttg acattaaaat
    33301 tcttttacat ccaaacaaat aattaggtgt gagcttttct ctgagaattg gatccttgat
    33361 actctttttt aaatattctc tcgccgcttg ctcaaatgct ttcataattt caggattgat
    33421 cacaagtcct aaaattcgaa tttctaaaac ccaatagata aattctcgat aaatttgctg
    33481 tgtgaaaggg aggatactgt aaagtaaacg ctcttcatca gaaattgctc tatcttgttt
    33541 tgaaatgatc caaggaggcg ttctctgaaa aaggtttaaa gttttgacga caggtgcaat
    33601 agctggcaca atttgaattg cacttgcccc agtcccaatc actgcaactt ctttgccttg
    33661 caaatcaaaa gaatgatccc atttagcaga atgaaatact tttcctgtaa aattctctaa
    33721 ccctggtaaa tcaggaatac taggacgact gagtccacca tttccagaga ttacagttct
    33781 agcaaataac acttctccgg aatctgtttc tacttcccaa tacccgttct cttccttaaa
    33841 ataaatccct tttacatttg tattaaacga aatcttataa cgcagattgt atttatctac
    33901 acagaaattc atataggcta gaatctcttt ttgttcgcca tacatttttt tccaatttgg
    33961 atttggctca aaagagaaag aatagagatg agacttaacg tcacattctg ctcccgggta
    34021 agtattgtct ctccatgttc ccccaattgt atctttttgc tctaagattt tataagaatc
    34081 aattcctgct tgcttcaatc gaattgccat acaaattcca gcgaacccag tcccgataat
    34141 aatagaatct aatattcttt gcttgttatt ttcagttgga tctattttgc gtgttgtcat
    34201 ctagctctcc ccttttcatt tcaggataca cgatttaaaa acattcgtcg cctgaactta
    34261 taatattaaa caaatttttc tgtctgaaat tataatttta tacaaagaaa atttttagaa
    34321 tttagattca aaatctgaga ttacacaaaa tatagaaatc aatccgtata ataaaagata
    34381 ttattatatt aggtttttct aatagaacaa tgaaaaacgg atgggatccg tccaagatac
    34441 taataggctc actcaatttt atgcaattgt gtttaagttg agttaataaa tacaaacaga
    34501 gcccatttcg gatttttttc caaaaagtgt tgacataaca tatattaagt attatgttgt
    34561 ttagtatgaa agagcttaca gacaaacaag aacttgtatt aaaatacata accaacataa
    34621 tcaaagaaaa aggttttcct cctaccattc gcgaaatcgg tgatgaattt caaatcactg
    34681 caaaaggtgc ttacgatcat ctcaaagcga tcgagaaaaa aggttatctc aaaacaaata
    34741 aaaaccaatc aagagcaatt gagttactaa gacacagcgc agaggatgga attccagttc
    34801 gcgtgatgag tatccctctt gtaggtagag ttgccgctgg acttcctctt tttgtagatg
    34861 agaatatcga agaatacatt cctgttcccg atcgtatggc aaaaaaagga actgtttttg
    34921 ctcttcgtgt tgttggagaa tctatgatag gtgagggaat caatgatggc gatattgcaa
    34981 tcattcaaca aaaagaagta gctcgcaatg gagaaattgt cgtggcactt gtcggtgatg
    35041 cgccggctgg gtcagaagcc actctaaaaa cttattttaa agatggagat accattcgcc
    35101 tcgaaccaag caatcccaaa ttcaaaacaa tccgaaccaa aaaagataat atcagtattt
    35161 taggaaaatt agttggtctg tatagatttt attaatctaa gctctcttcg aattcctatt
    35221 gacaaaaaaa ggaaactcaa acactcataa atgaatgaag agtttccttc tatttttttc
    35281 cctattctta tttctaattc aatcttgtgc tcctaagaaa gatcaagtat cagacactga
    35341 cctaaaaaga ctcattgatc gagtctcgat ggttcgtctt tctcaaaatc tagatttcga
    35401 tccatcggag ggaaagcctc tgaaaacaga ttatcaaatc ttcaaagaag tttgtgaact
    35461 tttccgttta gactcaacac aagcattaga aaaattaaaa tcacaacacc ctgaactcta
    35521 taaaagtata atggcaaaac gatgaaaaac aaagaaagat atatatggat tagcctcgtt
    35581 gcaattctaa gttgttcgat attccaacca attgaacgcg taaaagcaat ttcaaccgaa
    35641 ggggagaaat acttacaaat tttacatgaa gtaattagct tcattgaaac agattatgta
    35701 gaatcggtcg atgaaaaaaa tctatacata ggtgcaatca aggggattgt atctagtctc
    35761 ggagatcctc acacacgctt catgggagaa gatgaattta aacatctaca agaagaaacc
    35821 cgtggaagtt tcggcggtgt aggtctagag gtcacacatt cagaaggatc gattttagtg
    35881 atctctcctt atgatgatac tcctgcaatg aaggcaggaa ttttacctca agatagaatt
    35941 ttagaaatat ctggaaaaag cacagagaag atgtcaatcg aagatgctgt gaaacttatg
    36001 agaggaccag caggaaccaa tgtcactatc aaagtaaaac gcaaaactgt aaaagatcca
    36061 tttctactaa cgcttactag agaactaatt aaaatccaat atttaaagtc agcctattta
    36121 gaaaatgaaa agatcgggta tgtaaggctt actcaattta tgggtaggga aaacaataca
    36181 ggtggagaat tcaaacgaat tgtgcaagaa tttgccgcaa aagatgcaca aggaattatt
    36241 attgatttac gctctaatcc aggtggactc ttagatctag caattgagct atcagacatc
    36301 tttttaagac ctgatttaga cattgtatca gtaaaaggta gaggagaaaa actagttcgc
    36361 gttttcaaat ctcaagatgg tcaaggaaaa gttttagatg ttcccgttgt agttttactc
    36421 aatggtggct ctgcaagtgc ttccgagatt ttagcaggag cactccaaga caatcgaaga
    36481 gcattgattg taggaaatca atctttcgga aaaggttccg ttcaaaatat atacaatcta
    36541 ccttataaaa cggggattgc acttactatt caaaaatact acactccaag tggagtctcg
    36601 attcataaga aaggtattac gcctgacgta gtagtgaacc aaattacccc tgacaatgaa
    36661 gacaaagttg taatggagaa attaagtaaa acaactctca ttcaaaattt tgtaaaagac
    36721 aatccaggtt ataatgaaaa aaacattaag gcttttcaag atatgctttc taagaaagga
    36781 tataaattaa ataaaaccat ttctaggttc ttactgaaaa aagaaaatgt aatcggtgaa
    36841 aaaagccctg tctatgatag agaatttgat ccacaattaa acaaagcgat cgaactactc
    36901 ttaaatccta caaacccacg acctacttcg atgtaatgtt tggaattgga atagaaacaa
    36961 gttgtgatga aacttccatc ggaattgtag aagatggaag taatttaatc tcactgaaaa
    37021 tttttagtca aattgaaact catgcagaat ttagaggggt agttccagaa attgcctctc
    37081 gcgcacattt agaaaaaatc aatatactct tagaagaagc aattgcagaa tctaaaatag
    37141 attggaaaga tttatcttat gtagccgtca caaatagacc aggtcttctt ggatctctca
    37201 tgattggtgc tcaacttgcc cgttcgattt ctctggttca taaaattcca attgttccag
    37261 tagatcatct agaagctcat ttaaacactg tgcaattaga aaaaaaagtt cctaaatttc
    37321 cctacctagg tgttttatta tcagggggaa attcttccat cttgttggta gaagattacg
    37381 gtaaaatgaa attaatcgct gatacttcag atgatgcgtt aggagaagca tttgataaag
    37441 tatcctccct actaggacta ccgtatccag gaggacctgc agtcgagaaa agggctaatc
    37501 agcataaacc gcaggaaaat gaaaagaatc catttccaga attactgaaa gactatccac
    37561 aagagagaat cgaattttct tacagcggtt taaaaacaag tgttctctac tatcttaaaa
    37621 aaaatacaat cgatgaaaat aagatcaatg aagtatgcta tcattttcaa aatactgctt
    37681 tccagctcgt agaaaaaaat attctaaaag cagtaaaagc aactagagtc aagcaagtag
    37741 tagcaagtgg tggagtccta gcaaatgaaa ctcttagaaa aaaactaact tctctcgcag
    37801 agaagaaaaa attagaatta ttctatcctg agaaaaaaat actctgcacg gacaacggag
    37861 ccatgattgc ctctctaggt tattatttat ttaaacaagg aaagatagat tctatcgatt
    37921 ttaaagtaag tccgagtcga taggataaaa atgaatttac actgcatgcg aatgctgtag
    37981 attgattttc aaattaaaag ccctgaagga aaactatgaa attaaaactc aactgggttc
    38041 cgaatgctct atcactcgga aatttaacac taggatttgt atcagttcta atcgcatcag
    38101 aggcgagtct tagtaaccca aacaattcac agattttttt actctctgga atttttatta
    38161 tactagcagc cttatttgat ggattcgatg gaatggcggc acgtgccttg aatgcaacat
    38221 ctgctctcgg tgctgaacta gatacactgg cagatctgac aacctttgga attgctccag
    38281 ggtttttaac ttataaaatg attttagaaa attttaaaat tgatttattt ggaagtggag
    38341 aattatttcc ttatggaatg ttagtggctt ctatttttcc aatctgtgcc gcctatcgtt
    38401 tagctagatt tactgtcgct catgatccag cttcttttac agggctccct tctccgattg
    38461 cgggagtgat cgtaggatta tttccggttg cctttccaaa taatacgatc tctgcttact
    38521 taaccatatc tagcttcatc gcagttgcgt tacttatggt ttctaaaatt aaatattcta
    38581 aaccacaagt tgcgattcga ggaaagttta caaaggggag aatcctcatt atcctcggag
    38641 gtttcgctgt attgtttttc tttttaggac tcgcaaaagt tccttttgtc atgtatggat
    38701 tgttaatttt ctatgtgtcc tcaggcttta ttagcttaat catccaactc atccaagact
    38761 actaccccga caacgaatct taaatcaatt cattcatcgt taaaaaagac gcacgccgtg
    38821 cgtctctact ttttttcagg gctcgcccta gatccttttc gcatcggaga agaaggattt
    38881 tattggtctc taatatcaaa atttatttca gttcgaaatt ctacatgtgg taaatcaatt
    38941 tctttataca gtgccatttt attattaata attaataata tatttccatt accagaaata
    39001 ttacaatcaa ttgtattaat atcttcgttt agaatttttc cgaaaccata gtatacattt
    39061 tgtaattcta tcttttttcc gataaatttt aatttgccat aaattaattt cttggacaat
    39121 aaaacaacat cttcatactt tttataatat aaaatttcca ttttcatcaa tatgaactct
    39181 cctagacaca tcgcagtttg ggacaaaatc ccatgtgcca cttagaacct taaataaatt
    39241 cttttcagat ttatttttaa tcaactggtt acaaaactgc agttggtttt ctgggtatat
    39301 acaaaaagaa tgaaatgaaa gtcttaatgg agcatttgca gcgtgtttga gcaatgaagt
    39361 tcgctgctgc gggtttcttt tcttttttgc atactttttt cttttcttta caaaaagaaa
    39421 agaaaaaagt atgaagaata ccccgcggga gcggaaataa aaaaatctta tcttagtaat
    39481 tatagaagtc tgcgcgactt gacaaagtcg ccctaaaaat tagatcaatc ttcactcaga
    39541 gtttccattt tttgaatgcg aaagcggata acgcgatttt ctggatcaaa tttatcttta
    39601 cctaaatatt cctcaagatt caaatcacca actgctttta gtttgtggtc tggaattcca
    39661 ttttgaatta gaatatttcg aaacgattct gctctcgcaa gtgagatctt ttctctattt
    39721 tcagggagtt ctgtcgcgtc tatctttcct ctgatttcta aaaaatatcc ttccggaatt
    39781 cttttgacaa tcgtagagag agtttcgcaa aattgattac accactctct aaaatagatt
    39841 tcattgattt cagaatgtgc aggagaaaaa gcagagtgat aaggagtttc tggataacga
    39901 atgttttcta gtttataatt gtcaatatag atgcattgca aaaaaaagaa tagaagaatg
    39961 aatttttttt tcatacgaac tcctaattat cactctaaaa tttatactcg gcggtgagaa
    40021 atgcttcttt gaaatagctc cctcccctat tatcgagtaa tgcgcgcaga ccatctccta
    40081 cctgaaatag tcctgatcta aataaaagtt ggaagtcact caatacattc catcgtaaat
    40141 tcaaattgta ttctcttccc ataaaattag aactatcctt gttatagaat acattaaagt
    40201 ctcgattatt ttcaatataa ggagatttat ttgcatatag atggtaatat ccaagcgtta
    40261 gttcaaaagg tcctggtatg attatattaa caaaacttcc tacctcgtaa agcccagtca
    40321 tatttcgtgc actaaaaaga gcatagcctc ctgtgaagtc aatggcaata tttgaaattg
    40381 aaaaatcggg aaataaagtg cgatagccgc ccgcatgata tgaagttgga gttccatctt
    40441 tactaaaacc agggcgacct gttgttccaa ttccaattaa attcagtttg atttgaggat
    40501 tgtaactgta ggatatttga aaatcccaaa gtccaccact aatattatat tgttttctat
    40561 gttggtaaat ttcctgttca gaataatttc gaattgattg taaagctttt acacttcctc
    40621 tgttgtaaat cccatgagcc atgatagtca caactccgaa tctccattca ttaaaaagac
    40681 caaaccaata taagtttcca gtctctctaa aaggatctgt ttgatcgtta tctgtagatg
    40741 aatattgata aacttctagg gtataatgat tgactggttt aaatacagct ttagaaaaaa
    40801 atacattcac attttgtcgt ttatcattga atccatttcc atctagatct gccgctgttc
    40861 tctcccttgt tcgaatccaa cctccttcga gcgttagttt atatcctgga atcccttttg
    40921 tcataagagc cccacttcca atagcaaaca caagacgacc taaaggagaa gaaaagaatt
    40981 ggattccgta tctagaagta aaatcatatt cctttaattt aaaattgata tataaaaaat
    41041 tgcttctaat attaacaggt gtttgttgtc caagttcccc ccctgttcca cttcctaaaa
    41101 tagaaggatt atttcgatcc gtgtttgaaa ttgaacttcc tccaaacgtc atatccccga
    41161 tctgaacacc ggcaaatcca tcaaaatact tagatgtaaa aaactcaaag ttgagtaaaa
    41221 ccctagaatc ataataattt aatttttcat ttcttcttga aagagttgtt tgtttaccaa
    41281 cggaacgagc cgcaacttct tggttaaaaa tatttgctcc agtagcggct gcattgctat
    41341 attccagttc aggattataa taaggtgttg tagaactctg acgggaaaga agtgtatccc
    41401 gactaagtgc aaaacctcta aaacgcatta cacctgtaat attaaacttt ttttggggtt
    41461 ctgtatcatc tgaatataac gcttgtagaa ataagaaaca tagagccaga aaaagtttca
    41521 tgccctagcg aacccagtca ttgttattaa aatatttttc ttttaatcga tcaatctctc
    41581 tggttctttt catttcccga ataaaaaaat ttgcttcatt catcaacagt aggtcatact
    41641 ttttcattga gatactgagt tgtttttcaa caactggact tacaacagcc tgcattttga
    41701 tttttaaagc aggatttctc tgtagcaatc cttctaaata taaattatca gcaacataac
    41761 aattgatttc gttgtcgttt aacgctttta gcgcagcttc atctgtttga tacggcttaa
    41821 tcgtgaaacg ggggaaatta tctttcacaa agtcataatt ggaagtattg ttctttactc
    41881 caatgataag accagaaaga gattttagat catatagatt tcgaaaaatt ttattcgtta
    41941 caacctgccc ctctgattcg gctggtagga tagatttatt tactaatgca cctggagagc
    42001 tgatataata agaatcacta aatccaagct cttgtccccg cttcatacta gaagaatttc
    42061 caaatgaaat atctactcgt ccagatttga ttgctttcgc gtggtcagca aaagttaaaa
    42121 ggggaactac ttcttttaaa ttcacaccca aataatctgc aaacgctttt gccagctcta
    42181 cttcgaatcc aggatagcct ggctttggat tttgaatgta atagggttca tagtctcctc
    42241 ctactgagac tacgatctct ttttttttga gtattttata gagctgagaa tcctcatttg
    42301 caaaaacaaa aaacggaaga aatagaataa ttatacagac atagatatat cgtgaaagca
    42361 taatcactca ataaattata aattaattgt tttgtcgata atattataat tttttagaat
    42421 cactgtatcc attgtcaaga gtagaaataa acaagtgata aaaacaagaa ctcctaccgt
    42481 agaaagaaca ataaaaaaca aattctttaa aaagcgatat tgtaaaaaag aaagactaac
    42541 caatatattt cgtgtaacgg aaagaccttc ttctttgcca attttgtagt aattccttac
    42601 atagaaaata cacagtaaaa ataataacac gatcaataag attccaaatt gagaagtata
    42661 atgtttttgc agagaaaaaa gtgggatata aacaacgttt cctacaaata ctccagcaaa
    42721 gattatcaat aaaaaatgac ctgcgatttt taaaaacgag gattctttct ttggttcagc
    42781 cgcacctaat gctttgatag ttggtcttgc attgttttcc tcatcattaa aagatgcaag
    42841 cagcagtgcg ttaaatttat aatcacttgt atccactaac tttagataca aactcaatcg
    42901 ataaacaagc catagatata aaacataaac taacccatat ataatagctg aaataccaat
    42961 caccattgat ggcaatacaa gagagttaga attggaaaga gagctttttg ttgtatctaa
    43021 aaaaaagaat ccagcaaaaa atccaattcc aaataagcag atcgttgtga aaaacaaagc
    43081 tttctttgta aaaaaagtta caaagaattt ttgaaaggta tgtaggatag acatacatac
    43141 aattacataa acaggtaaat gaagtgcaag catcggtatt ccgataaacg gaagatgaat
    43201 cgataaaaaa atcgacctag ccaaataata agaaaatgtt aaaaaggcaa ctgagagcaa
    43261 aactctttgt aaaaaaatag caaaccaagc gttatttaaa acagatacaa cagttgaaaa
    43321 acaaagtgca attaaaaata cattcagaaa tagttttacc ttctgtccta taagtaagcc
    43381 ttcgatcttt tcaattgttt tttctaattt gccatcactt ggcgcataat cgtcatttac
    43441 ctctttcaga agagagttta aaaagaaaat tttgaaaaca attccttttc gaatctcaag
    43501 agaagcaggc ttttcttcca agtcttcatt ttcaattcta gtttccagtc gcattacatt
    43561 cttggtatca aaatcgactg taatattgag aaaaatatcc tctaaagcaa ataagaatat
    43621 aaggaaggaa attaaaataa taaatttgtt agttttgttc atttgaattt tatagaaaaa
    43681 tataggttag tatccaagca acaattaaga atcctacaac agctgctaca ccaatggttc
    43741 ctactttaaa atgaaattgc tttttaggtt tgtcttctag atactcttca tcattttttt
    43801 tctttatcgt tgttgcaagt gtttcaaatc cagctagttt cactttgaca gtcgtttctt
    43861 tttcggaata tctcccaaaa actccatctg ttacttttac taccgcatct acctctgccg
    43921 gacttttttt aatatcgatc actgttgcag gaacaggtcg aattcctcca ccctcttctt
    43981 ttaaagaaag tagattgata acgttattag aaacttcatt gtctggtgtg aacttgatca
    44041 taatacgatc accgattcga agttcgtcta ccctagttcc tgtcacagga gaaagaacgt
    44101 aggtaaagtt tccaactaaa gaaccagcag gaatcaatga atcaacaaag tctttgtcct
    44161 tagctttgtc gattatatct tcccgaacag aagaagcatc aatttcgtct ctttggattg
    44221 gatgaaaaat tgtattaatt aaataatccc cttttcctaa aaccttggaa agattttgtt
    44281 tgagcaattc gtttagcttt tctgaattat catattctag ataatacatc atttctacta
    44341 gatagctctc tgaggcaaaa gagttcttaa aggaattgat cagagtcaag gtagcactct
    44401 gaatatgatt tccctcaaac ataaatttct gaatacgatt cactacatct ttataggtat
    44461 aatctgaatt aatattaatt aattcatttg aatgcgaaac caatgtttca aaacgttcta
    44521 ttgtattctt ctgtttattt gcaataccag caaatacaac aaaggttctc tgtatactat
    44581 cacgtgctac aaatttaata aaatataaat cacttgccat aggggactat ttaacaaata
    44641 aaccaattcc tatttggatg gcaactacta ttataatgcc atatttctaa tctcaatcct
    44701 actcatccat tcatgaattt ttaaacagat taaaaaaata cttcacagaa accatttcta
    44761 gctctcagaa attggatatg atttgccaaa tctatctgtt ttatgaactt ggtaggatat
    44821 tccgagaaag tgaaactttc tactgtagag aaaaaaatgt ccattgtaaa aattgaaaac
    44881 ctgaaaaaat cctattatat atcagataaa ggtgttccta tcctttggga tctaaactta
    44941 aatgtgggag aaggagaaat tgtttcagta gaaggctctt ctggagtcgg caaatcaacc
    45001 cttttaaata ttctaggagc catggatgaa ttcgatagtg gactagtaga ggtatgcggc
    45061 accgatttgg gtaatatatc agaaacagac aaagaaaaat ttagagccag taaaatttcc
    45121 ttcatctttc aacatcattt gcttctccct gattttaccg ctttagagaa tgtgatgatt
    45181 ccacttctaa tcagtaaaat gaatgtgatc aaagctaagc aagaagcaac tgcaatgctg
    45241 gagcgagtag gtctaggaga aagactcgct agttttccat cccagctctc aggaggagaa
    45301 agtgcacgtg taggcgtagc gagagcactt gtttccaaga agcaattagt cttagcagat
    45361 gaaccaactg gaaatttaga ccgagaaaat tctaaaaact taatggcact cattttagaa
    45421 ttacagagag acctcagatt ttcgatgata cttgttacac atgatatgga acttgccgct
    45481 atggcgacca aacggaataa attactctcc ggaaaactac aacctatccc ggtatagcat
    45541 aacgttctca aaactttttg aaagattacc accttttgcc gcgaacaagt ggcaatggat
    45601 tttgggtgga gacaccgata gtcaccgatc aagaaacgat tgcaagtctt ttagaatgag
    45661 acgttttgaa ttagatgagg taaattttat cactaagaaa tattaaccat aattaatata
    45721 actcaaaaaa tagaaaagac gttatctctt aactccggta tttatatcta tgatagctat
    45781 cctattggca acggtccctc cattgacact agtaaaattt cctccgatca atactgctgt
    45841 cccaagtgta ataattgtat tcactacatt gtttgcattt ggattccaag cagtcgcatt
    45901 tccggtcaaa tccgttgcac caatataatt tctaacgaga gaaacaaaag atgcagcgcc
    45961 agtaaaagca ccaccaacat aaatatttga tccagaaatt gcaattgcat ttgtgatatt
    46021 atttgtgcct gtatttactc ctgatgcaaa gccgggagta gcattacctg tcgagatttg
    46081 aatagaacca aaattattaa aagtagtccc acaaagacct cctacaaatg aacccgcaat
    46141 taaaacatct gtagatgtag caactatcgt atttacggga gatacggcgc ctgtagcgca
    46201 ccaaccaggt aatgttaatc ctgtagatag atctacagcg accagataat ttctattagt
    46261 ctgaccgcct atattagcag cagcaaatgt tccaccaaca tacgcaacgt ttccgcttac
    46321 agcgattata ttaattacac cattattaca attaggatcc caagctgttc caattccagt
    46381 atttaagtcg atagccgcaa tcctactcct aggctgccca ccaatagacc ctcctccatt
    46441 aaaacttcct cccacatagg caagattgtt acttattgca attgtattca cagtattatt
    46501 tgcgttcgga ttccaggatg tcgcttgtcc agtgactatg tccaaagctg caatgcggct
    46561 ccttgcttgt cccccaatat ttaaaaatgt tcccccaaca tagacagtgt ttccattcaa
    46621 tgcaattgta ttcacagtat tatccgcatt tggattccaa gtaggaattt caaagccatt
    46681 tactaaatca atagcggcga ttctatttct agccgaagat ccaacagaag taaagtctcc
    46741 accgatgaaa agaatgttgc agctaacagc cattgccttt acagtagtac ctcccaaagc
    46801 acccaccttg agtactgcct gttgtgtctg aaaagaagtt gatccagaat aggaagatgc
    46861 aagtgcattg cctagagtat ccttaactcc gcttaagact tttatttgca gcatgtaact
    46921 agactcaacg caaaatgaag tttggagaga aatacttgca atcatctgat ctgtggaaat
    46981 atttaggata ccacctaggc aagaagtaaa gttatttgtg gataattgta tgttacccgt
    47041 acacaatcca tctgtggaaa tcattgtgag agtggatgta tctatagcct tatcaaatga
    47101 aatagtgatg ggtgttccta ttataaatcc aaccgtatta tttgcagggg taacagaaag
    47161 aacattaggc tgtgtattcg tggcagcagt agaagaggtg ttaacagagt ctttgatctt
    47221 agagagatat actgcaaaca tagtatctac atttcctttt gaaatagaac atcctacaaa
    47281 atagagtata gatacgcaaa ataaaagaaa taactttata tttaaattag gaatgttcat
    47341 attagttaaa acaaactccc tttctttagt atcgaaaaag tgaattaaaa attcaaatca
    47401 ttgaattgag ggctaaattt aaactaaatt ggaaagtagt caaggaataa attaaaacca
    47461 cctatgatta tagcgcctat cgattcaaag tcttctataa tcttctccga ttcaagctta
    47521 tgaatgaaaa gcttagatca tcaacgtctt tctggctcta tcgctaattt gcatccagct
    47581 ttctccataa tcatctcctg tttctatctt acaagtgaat ggcttaaatc aaaaaagaat
    47641 tgcgagtttt tttagaatat ctcatttgag aaaatctcaa aaattaagaa agcctttctt
    47701 taacttgtaa tttgtgtaag ataagttatt aatttaaagc tcaataggct tggtaaccga
    47761 atcgcttgtt cgtgttaaac actggattta ttttagtagt gatagaagcg gcagttgtgt
    47821 tggaaattcc tgtcgttcca ttcgaataca taatgtatac tcgtccgtct tgtgtattgt
    47881 ttaacacgtt agcagtagca attatatccc catacccatc cccgtttata tcagagccag
    47941 aaattccagc agcaaatcta ccaccaccac cgacttcacc aattataatt ctatttgcag
    48001 aagttaatgg gacgctggga acgccagagc tagaagaatg aaatatatac atttttccat
    48061 cgcctgcatt aatataagca gagcccagga ttatatcctt aaaaccatca gcgtttacat
    48121 ccattatcca cacgaagcca ccaagcctag ctccactact ttctccgaca taagaagcat
    48181 gaggggtcac atttattcca cttggtccat tggataaata aacaaatact ttcccaatgg
    48241 ggctgcccgc gccattttga tctgcgccaa caattaagtc tgcgtaacca tctccattta
    48301 tatcccctga attgatcgag taaccgaaac tgtcttgatt ggcagtaccg gctatgcttg
    48361 ctaccgcaga agtagtatta gccgcaataa ttccaccaga gccagaagaa tggaatataa
    48421 agactttccc tttatttgtt gctggcgcgg taatgcttga gacgctcaaa tcagtaaacc
    48481 cgtctccatt ataatcaccc atggaaacga aattactaat atcctcactc cctatgccga
    48541 ctatcgttgt atttgctccg gctaaattat tagcggtaat tccagatgcg ctggaatgga
    48601 aaacataaac ggctccagta gaggcaatat aattattaat agcaacgtca gcatatccat
    48661 ctccatttac atcaccagtg tctaaaccat agcctaagta tcctaatgca cccccaacaa
    48721 ttgattttgg ggcagcactg gcaagcgttg tggttattcc attggtgccg ctagaataaa
    48781 agaaataaac ctttccagcg cttcctgaaa aacttatatc actcactacc aaatcagcat
    48841 acccatctcc gttaagatca ttggttacaa taaagtctcc aaaggtagta gtcgtttctc
    48901 cggtaatcgt agtattagcc gctgtagtag atgtagcggt aattccattt atgccgctag
    48961 aatggaaaat atatacaacg cccggtccta cagctataga ggaagctacc atatcactat
    49021 acccatctcc atttatgtcg tggtttttat ccttatatac cgtgatcgag gttacaggtg
    49081 atatgccgcc ggcagtatta atagctctaa cgttgattat atgtttactt ccatctttcc
    49141 acgtcaagga tcctgaagga agtttcattt tccaactagt agttccctgt gcagttgaat
    49201 agttaccaga atcaaaggca acttctacct tgctcactcc caagctatca gttgcagttc
    49261 cgatcatgaa tccagtatat acagtgctct tgtcttttaa gttttgtatt gtaacagttg
    49321 gaacgttagt agaagagttt atcgattggt tgtttaaaga tttaattcca caaagagaag
    49381 tattatctcc tgtaaaggat ttaaaaatta atccatttat aaaattgcga ttcttcggat
    49441 cgcatgcgtt cttataagtt gcctcaccaa tacaagtaat gcttaaaata gaaatggaaa
    49501 aaataatttt taatcgtagt ggaattttca tattattcct ttagatggtc atcacttaga
    49561 gttgtttctt cccatcggtc aaaatctgtt tgtagttcaa gaatcttatt tcgcgctcct
    49621 cttaaggaag cggcaactag tagcaaacga agtggtggct tatttgattc tgccgtttgg
    49681 atgatagctt ttgctactcg caatggattt cctgtttgtt taccactagc atttcgaata
    49741 ctattttgat ttgcatttac tgtttctttg taatcttcga ttttcatttt ctttctagcg
    49801 ctctctttaa tttccatcca actttttcca taatcgtctc ccatttctat cttacaagtg
    49861 aatcgcttaa ggcaaaaaag gattgcgagt cttttagaaa agatgttttg aaataaatag
    49921 ggtaaaattt attttattcg tatctcgact tcgctcgata aacggtttct tggctttgat
    49981 cgattaaagg ctttagtata gaaatccatt ccactatccg cttgcatctg tggttaaata
    50041 tttttaaatt gagttcatta aataacgtga attcgaaata aaattttttc accgccgata
    50101 actgaaagat acagaaacga tggttactgt tgaactatcg tatcttttat cggtgtcctc
    50161 ggtgtatatc ggtggtaatc ttttaatttc tttgtaagat cgaactcgcg ttaaataata
    50221 ctcaggaact tttctacgtc tttatgattt ccaaaaacaa ccatgatatc tctcgctcga
    50281 aatacagttt ctgcagaagg gataccaaga gtttctaaaa ccacatcatc ttctcttctt
    50341 ttatttttag aaagattttt gtgacgcttg attgtaatca gaagtaagtt gtatttttct
    50401 tttagcttta cttccataat tgttttacca ttgaaaaatt caggaacaag taattctgca
    50461 atccgatatt cttctgataa aataatactt cctttgatat ttgcatggct aaacatctca
    50521 gccatgttct ttgctgctct ctcttcggga ttaaaaatat gtgtgatacc gagcattttt
    50581 aaaatacgaa tatgaagtgg tgtttggtag cgggcaatga tttctttcac accgattcgg
    50641 cgtagtatat cacttgtaat aactaacgtt tcaaaatcat cggcagtggc taatataaca
    50701 tagtcaaagt cttctagtcc ttgcgcttcc atagcacttt catctgtaga atccaagcaa
    50761 actgcattcg tgcaaacgtc tttgatttct tcaaccaatt ccatattttt gtcgatcgca
    50821 attacttcat gcctttcatc atatagacat tttactagtt ctttcccaaa atctccaagt
    50881 ccgatcactg caattttttt tgtattcata aaccctactg taataatccc actatttcct
    50941 tgtaaccaag atctttcgca taatccattg ccgttaaccc atgaatgtca gtcatgtttc
    51001 tatctgctcc tttttctagg agatatttca ctgagtcata atcgcctaac gctgctgcaa
    51061 tcatgagtgg agttaaacta tttttgtcct tttcatttat atcaatgttg gaatataata
    51121 cctgactttt tttaggataa tcaattaaaa cacgcataac atctctatat cctcttaaaa
    51181 cagaaagatg taaaagatta gaaccgtcaa ataatttttc gttaatattt gcattgctct
    51241 tcaaaagact cctaacaatt tccgcatgac cattttctac agctaaaata agggctgtct
    51301 tatgtttacg attctcttct gccaagcgtg gttttctttc tagaagaata cgtgtaattt
    51361 ctttattccc tctttcaatc gccatcatga gtgcgttatt tccatctgag aatttaaaat
    51421 gaggatttgc tcttttcttt aaaatcattt ctgcaatttc aaattcatct ccctttacgg
    51481 aagccataag gcttgatcca aaatgtttat ttaatacatt tacatgcact cttttttcta
    51541 atagcatttg cacaattgct ttatgaccat aacgtgctgc gataaagagt gcattgtccc
    51601 ctgcttcgtt ctttgcactc gcccgtgctc cattttctaa aagtattttc acaatctctg
    51661 gcttaccaaa aaaagcagca ctcataaggg ctgtatttcc tgtcttagtt gaaaaattaa
    51721 cttccgcttt gtttgcgaga agcatttgaa ctaactccaa atttccccta tcacaagcaa
    51781 ttataagtgg tgtttctaac tctaaattat aagtattgat ttcagctcct ttagagagta
    51841 agtcttttac ttctgaaatt ttttcgtatc gaactgctag ttctagaaaa ctcattcctt
    51901 tatcattttt ccaattgagt ggcttatcta aaaattcctt tcttaaatat tcatattgat
    51961 ttttatccga ctcttctaag gctttttctt ctgcgacaga tttcattacc acgcttagta
    52021 tttttccata gccatttgct cttgctaaat cgagagcact aagacctgat ttagctttta
    52081 tatgaatatc cggatttttt gtcagaagaa gtcttatgat ttctccctca ttttctttag
    52141 agatacaaac catatagaga ggagttctgt tctctaagtc tcttgcattc acatcagcgt
    52201 tatacgaaag aagtaggcta acaattcttg cattcttatg ccaagcagac gcatgaaggg
    52261 gtgtaagtcc tttttcattt gcttggttta cttttgcttt ataaaaaaga gctgttttta
    52321 caagttcata ctgattataa taagcagagc tgtataaaaa actatctcct gcatagtctt
    52381 tcgtatttac atctgctcca tttgcaagaa gaagtcttgc tataaataca tgcgagggat
    52441 aaatgatatt atccttttcg agtcttgcaa aatgtaaaat ttgtaaaaga gcatcagatc
    52501 cagaagaatc aattaatttt aaatctgctt tattctgaat tagatactta gcacataaaa
    52561 agcttccata aaaggatgca tacataagag ggcttttgcc cttttcccca tattgctcat
    52621 tgatttgaat ttcacccgaa ttgaggagag aaattagcct agataagtct tctttctgaa
    52681 ttgcttcaat tagattctga ttttctggtt taaatatacg atcttctttt ttaataacag
    52741 tctttgttgg aactatcggt tttggaggag gtttgtaagc aggatttaag atttgcacaa
    52801 cagcccagtg attgtttttt acggcgagag taagagcaga ctctttttta cgattcagtt
    52861 tttttgctct tgctttatta gttaaaagta aatttacaag atcagcataa cctcgttctg
    52921 ctgcaatcat catagcggtg ttgccttcag agtctctcaa gtttaaatca aaatggtttt
    52981 caataaaata ctgcatcgat ttaaaatcat tataataagc agcacttaaa actccataat
    53041 ctctatactt tcctctttta gttttctttt ttcgatccaa tagcatttgt gcaatttcat
    53101 actgcctatt tttaggatta cgaatcgcat tgtaaagagc tgtttcgtag gcttggctag
    53161 gctcttcaat ctcaattcct ttttctaaaa gttgttgaac taatttttta tttcccttat
    53221 agcatgcgag cataaacaca ctaaatccct tttgtgactt tgcataaata tcagctcccg
    53281 cttctaaaaa catatctact atatcttgct cttcagatag aatagcatac ataagcggag
    53341 tataaccttt attatccgca acagttaaat cagatttgcg atcaatcaaa ggttgtatca
    53401 catctctttg ctttttatct atcgcatgta taattgctgt cattccttcg ctatctcgta
    53461 aatttaagtc tggctttttc tctaaaatca tttgaatcat ttctttttga tcaaagtaaa
    53521 ttgcctgcat aagcggtgta tcacctgtaa aatttcgaac attgacatcc gcaccttttt
    53581 ccaataaaaa tttcactaac tcaatgtctt tattttcaat ggatatatgc aataacgtat
    53641 ttccatcttt atttctcaaa tttagattag cattttttga aattaaataa tcaaatattt
    53701 ctttgtcttt gacacgagcc attaaaacag attccccatc ctctttttga tgattgatat
    53761 tggctcctct ttctactaat attttgatga tagcaagctc gtttgttttt ccagaatgct
    53821 tatctgaata attcatgagt aaattcgcac catttacatc ttcctcattc acatccgccc
    53881 ctgagtcgat caagagatga ataccttcta caaaattttt ttgacaagca attcgaagag
    53941 ccgtttcatt tctataatta atcgcatttg ggtctgcttt tttgtccaga agtgctttca
    54001 tgatagagac tgctccgaaa tgagtcgcaa gcatgagtgg agttgtattc cattcatttc
    54061 cgccattgat atctgcacct gagtcgatgg cacggttaat gtccgttatg ctccttttcc
    54121 gaacagcttc aaataaatcc tcagtaggtc ctgaaaagag gtaagtcgta taaaaaaata
    54181 aaataaaaaa tcttaaaaaa attgccatgt atctagactt agaatatttc tatttctgga
    54241 aagttaaatt ttgtattttt agaaggagaa aaatatggat acatacaaac caatctcttg
    54301 tgaatttcat gatgacctag aggcatattc cgtattaaag aaaaaattgg aaattgttta
    54361 tgaatctgaa tcaggagaag ttcttactga attcggtaaa atcgttgatc tatacactcg
    54421 agacaaagca gaatttatgc ttttagattc aggcaaagaa attcgattgg atcaattgat
    54481 ccgagtagat aatcctatcg gataatgaat attagcctct cttttttaaa gcaaccaaga
    54541 tcttaaattt ctggagtatt ttaaaactaa ttcttttttt attcctaaaa gctatgagta
    54601 ttttttgaca gcccctttct ccctttatta cttggattac actccattaa aaagtttagc
    54661 aggaattgtt atttctttaa taatattaga aattacgtaa cctatcaggg tgatgaaata
    54721 aaaaattaaa ggaaggaaac aaagggtacg
//
LOCUS       DAOQOS010000022        53120 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_237_length_53120_cov_6.862565, whole genome shotgun sequence.
ACCESSION   DAOQOS010000022 DAOQOS010000000
VERSION     DAOQOS010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 53120)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 53120)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53120
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_237_length_53120_cov_6.862565"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(430..1245)
                     /locus_tag="PLF66_06730"
     CDS             complement(430..1245)
                     /locus_tag="PLF66_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015682001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HMZ63774.1"
                     /translation="MQTNELKTKAREIREEVIKMVTAAKSGHPGGPLGLADIYSALYF
                     KILKHDPKNPNWEERDRLILSNGHVCAVRYAAMALSGYFPVAELATFRNIKSRLQGHP
                     SITYLDGLETSSGSLGQGLSIATGIALGLRHQKKTNRVYACISDGECGEGMTWEAAQS
                     AVHYKLDNLIAFMDRNFIQIDGNTEDVMRLEPLPQKFRTFGWNVLEADGHNLDEILSS
                     FEKAQSHKGSPTLIVFRTILGKGVSFMENKPEWHGTPPNADQAKQALEELAKT"
     gene            complement(1247..1633)
                     /locus_tag="PLF66_06735"
     CDS             complement(1247..1633)
                     /locus_tag="PLF66_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF420 domain-containing protein"
                     /protein_id="HMZ63775.1"
                     /translation="MNLYLINAGMTLSILCFYIGYYFRRSNNTLHKRINLLGILFNLA
                     TAVFLLIHKYLINGIEAAGIFPSVPITIVNIHRAFAFLSLILMLLMGYTGITKNAKLH
                     KKIHFIFLPLYTIVFISGLFIFETRN"
     gene            complement(1688..3301)
                     /gene="murJ"
                     /locus_tag="PLF66_06740"
     CDS             complement(1688..3301)
                     /gene="murJ"
                     /locus_tag="PLF66_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002101545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HMZ63776.1"
                     /translation="MEVEKKTSSSASKSIALSFYTFLSRILGLIRDHYMAISFGTGFV
                     ASAFSVAYRLPNMYRNLLAEGTLSQSFMPLYSDASKVSEEEASLMSGAVLSFLCFMLL
                     VFVGVFVLFAPYLLPLLVGGTKEYSDLVIELSLILFILIFTASLSSIYIAISNSKQKF
                     FVPSLSPIILNLSYLFVFLVVFRFYEDTLERVKVLSFGIVTGGFLQLAVQAYYVKKLN
                     LFPKINFNWKHPAIKKIITLMIPAVIGGGFYQISLLVDIFLANYIQNQNPGLGAVVSL
                     DYSQRLIQLPTGIIGVALATTTLPGLLASLKEDKKAAIPSELAETLCFALYLTLPAAF
                     GFVFMGETIVDAIFYGGKWDSKATLSTIQALQFYSLAIPVFSMNKILTSSYYAFKDTK
                     TPLQVNMISFSINIGLNLILMNFLKHAGLALSSTIAAHITFSFLFFKMRKHEIRIPEK
                     EILFRLLKILIPLTALTLWLIFTENFLHLESFSFIQEMNLAHENIARLKAVVGILPAI
                     VLYFSFSKIAKLHEFDLIFGKILGKLKRK"
     gene            complement(3292..3918)
                     /locus_tag="PLF66_06745"
     CDS             complement(3292..3918)
                     /locus_tag="PLF66_06745"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020778909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HMZ63777.1"
                     /translation="MVESLKAEKLEELKKITEACVKCKLSTTRTKVVFGEGNPEAELL
                     FIGEGPGKQEDITGRPFVGKAGELLTRLIEKAMGVPRSSVYIANIAKCRPTEDLKMEK
                     DRAPDPEEVAACSPYLLKQIEIIQPKVIVTLGNPSTKFLLNTQEGITKLRGKWAEYKG
                     IPVMPTYHPSYVIRNGGENSKVQQDVWSDLLKVMEKLNWEPKVKIKWK"
     gene            complement(3918..6662)
                     /locus_tag="PLF66_06750"
     CDS             complement(3918..6662)
                     /locus_tag="PLF66_06750"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF012532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HMZ63778.1"
                     /translation="MAEENQSSKVKNDLIFLMNCFKEVLIELKEDEIAKNLPWVDGDR
                     TPHYFEKIDSNFPLRASQALSISFQILNMVEENAATQFRRSIEIEKGIDQIVGSWGQN
                     IRKLKNLGFDSTAISKSLAEIHVEPVLTAHPTEAKRATVLNQHRELYLLLVKLENSMW
                     TPAEKENLRNEIKLCLERLWRTGEIYLQKPDINSERKNIIHYLENVFPHAIALQDKKL
                     KQAWLANHFDPTLINRIEKLPKISFGNWVGGDRDGHPLVTAEVTKETLHELRQTALNI
                     IHKRLTDLTAKLSLSANLHSIPRRLNQFIEDWVEQMGDVGQRCISRNPNEPWRQFVNI
                     LIARIPFPSVEEKEYSYKNHFDLLKDISILRDSLLEVDAFRIVEYDVFTLERLIQSFG
                     FHLAALDIRQNSEFHDKALSGLMKASGSKNIYFADWNEFERLHFLNEELESARPFLAP
                     GMPLEGEAQAVRDCYLVISEYVKKYGRHGIGALIVSMTRKVSDLLVVYLFAREAGLVM
                     NTTEGLVCLLPVVPLFETIEDLENCATIMDSFLAHPMTRVSLAYQSFPDGIPTQQVML
                     GYSDSNKDGGIIASQWYLHKAQLELDEVGRRHGVKIKFFHGRGGTISRGGGKTNHFLD
                     ALPEGTLTGQIRSTIQGESIAQQFANLINATYNLELLVAGVTGVSQSHKKIGKITEEQ
                     EKVVHSLAEKSRRAYENLVRSEGFMIFYSQATPIDALENSRIGSRPARRTGKRSLKDL
                     RAIPWVFSWNQSRFFLPGWFGVGTALSDIQIHQPDVFQFLKKQIVSWPFFRYLMMNVE
                     TNIYNADKEIMQFYGHLVDDDQVRDKYLTQIVNEYDRTKEMLSLLFESKVEDRRPRMF
                     RTLAFRSSGLKSLHLMQINLLKQWRQNLQDQNSGSAEKLLIELLLSINAIASGLRTTG
                     "
     gene            7210..8310
                     /locus_tag="PLF66_06755"
     CDS             7210..8310
                     /locus_tag="PLF66_06755"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HMZ63779.1"
                     /translation="MAKKKSGTSSKNTSPNVEAVEETSSLPDPAAPPPTSTPVTSHHN
                     HLARGLLLILASSLLLSFQNVVTRVILSPKSLFGLFEGIVLNSSLSNSLLILVLRCAI
                     VLPIMAFIVAPWLHKNTWQDIKDLPKPENKTRLYSVLSSGLFLFFSQLCMYIALGTIP
                     TGIATTIFFIYPTITILLMWIFFADKPSLPLIFAMVTIYIGGFMTIPMTAFTPKGEGN
                     FVLGAATAALSGIAFAGYIIMIKNARMHPAPFTIVNFSIILILGGMILPFMDYSIDSS
                     HIMDLVYGTLILATTTLLGYLLQNFGVPLVGPSLASVIGASGPAVTTIMALVLIDETL
                     NLQQVLGVFLVTLWVLGISVENMKKAPVPQKK"
     gene            8533..9786
                     /locus_tag="PLF66_06760"
     CDS             8533..9786
                     /locus_tag="PLF66_06760"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HMZ63780.1"
                     /translation="MKNKILVILLYLIVIGFYAESSTKIDKSEMNRIVLWEGEITGIY
                     KDKGSVRALISHNPEWLGVNFEEIKNGILKKKKYELRQKVTNKKIGYFIADHVDLERK
                     IEKKQKTDYQVLIHGKFDLADPSYQNFVSNDFIIATVRYEDSYLDPSRFYKDNITSPK
                     QSIIHPVDRKEMVLVPASAFIHGQGASGELDSYNPAFQNPNDTNLLEISSFYIDKYEV
                     TNGEYARYLRDTNANPPLYWVDGKIPSGKEDHPVIALSYREVERYAAWAGKRLPTELE
                     WEKAARGQGFTRTLNRDESYTIQLHTVIYPFGNKFDSLLCNTKESKIGDTVSVYELAV
                     KGASPYGAIGMCGNAAEWTSSWYMPYDGHYLKNSSFGKQYKVIRGGSFYEDKNSAKVY
                     TRSFGGNPNLAEDRRAGFRLVKDLN"
     gene            9787..11115
                     /locus_tag="PLF66_06765"
     CDS             9787..11115
                     /locus_tag="PLF66_06765"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HMZ63781.1"
                     /translation="MPIPSITGWKALKKSSQLKEDTLGFFVSQFEEYGDLFHFNLLGL
                     NMFIMRDPILIRYVLQENNGNYTKSIFYKELERLIGKGLLTSEGSEWKKNRRLTQSAF
                     KKSSIEGFSQIFYEESDRLVGEWKDISEVDMSKEMMRLTFRIVGRSLFSAKLDEDTQI
                     VDESLGIALEEITRRIQSPIKIPFILPTPRNRRLKKAVHNLDSVVNKIISNRIKSGER
                     VKDLLDTLIYARDEETQQGLSPKQIRDEVITFLSAGHETTSNALTWTFYLLSEHSEVR
                     KKVIEEIRSNIPSTGQITISQLEKLKLTERVLKESMRLFPPAWIIERMSIKEDTIGET
                     KIGANSLVSVCTYAVHRNPKYWNNPELFDPDRFLPEEEEKRHNFAYIPFGGGPRVCIG
                     NNFAVTEAMTALACILRSYEPTLIAGHPVVKEPLVTLRPKFGMKMHLNKR"
     gene            11198..11326
                     /locus_tag="PLF66_06770"
     CDS             11198..11326
                     /locus_tag="PLF66_06770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63782.1"
                     /translation="MANQNDPSFLYRLVIGVIGGSLLNISSIELMPFKALVVFREY"
     gene            complement(11303..11740)
                     /locus_tag="PLF66_06775"
     CDS             complement(11303..11740)
                     /locus_tag="PLF66_06775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63783.1"
                     /translation="MTTTLKGFIISFSFLLILFLGYILFFGVSGLQELGAVLFILPAF
                     PVVGVLLGFFSKFIQQRRFFKLMGMGLISIYLIALLFSCILVLTDESKSIGFLETISS
                     IFLLGSAGFAIFGSFLLPLLAIGVFLLEKWTRENLNSILPEDN"
     gene            complement(11744..13003)
                     /locus_tag="PLF66_06780"
     CDS             complement(11744..13003)
                     /locus_tag="PLF66_06780"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HMZ63784.1"
                     /translation="MALPDYPLENQIERINIFKFSYNFILGVVVFFAAFDFLFIRFIP
                     SIHVLNALKIFVVILLHPFLKPLNISKAEDVQVKTFLAIVIGIPVNLFLIIGSREWLL
                     EQNVPIVKETYYYLLQYAVYLLLITAGLRLKTLNALVVSAVYILVYSFLLLLHKSVFT
                     SNEDIYFFLFQCSILALVPTVGLGMTYFVDKYIKNQIRLIDRADFWRNYAENLLRQIL
                     PAPKLGQPGLLPVTYYLQNITLLYFDITGIANYFRAKETLIGLDEFLKQLFSNAKDFC
                     IENKIEEHEEDGKYWYLVIDHPAGTNPLYADPLADLALHLQKKFQIICSENKLKLDLR
                     MGMASGKYVDYLTNAQKIDIFYINDSFKDADAMEKHGVNGEIQVTSATFELLKERYRF
                     VKRGPIALNKNKADTAVDLYLLQEKTD"
     gene            complement(13026..13099)
                     /locus_tag="PLF66_06785"
     tRNA            complement(13026..13099)
                     /locus_tag="PLF66_06785"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13063..13065),aa:Arg,seq:acg)
     gene            complement(13107..13520)
                     /locus_tag="PLF66_06790"
     CDS             complement(13107..13520)
                     /locus_tag="PLF66_06790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63785.1"
                     /translation="MESATIHQNKYLKRIKTYIDLGLNYVRSEEGMRDIAMLPLFFNW
                     LPFLIFKSENLVIRKSAIYSMLFSFYFFACFLASMALNLIPFIGSVFANIAHLVGILA
                     YLGLSGFFIYSYRRNKTLDMAVIDKHYEYIVTNLF"
     gene            complement(13540..13626)
                     /locus_tag="PLF66_06795"
     tRNA            complement(13540..13626)
                     /locus_tag="PLF66_06795"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13590..13592),aa:Ser,seq:gct)
     gene            complement(13638..13724)
                     /locus_tag="PLF66_06800"
     tRNA            complement(13638..13724)
                     /locus_tag="PLF66_06800"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13688..13690),aa:Ser,seq:tga)
     gene            complement(14025..15590)
                     /locus_tag="PLF66_06805"
     CDS             complement(14025..15590)
                     /locus_tag="PLF66_06805"
                     /inference="COORDINATES: protein motif:HMM:NF021349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HMZ63786.1"
                     /translation="MKRLPLGIQTFSEMIEDNHYYVDKTMFVHKLASVGKYFFLSRPR
                     RFGKSSFLDTLKEAYEGNEKLFQGLFLEKNWNWQKKHPVIKISFGGGIMQSSKDVLEK
                     IEGILQMNEKHLGISCQNKTISNCFTELIQSSFEKYKQKVVILIDEYDKPILDNIEKP
                     DVPYEVREILKGFYSVIKDSDNYIQFVFITGVSKFSRINLFSGLNNLTDITLDPDFAS
                     ICGYTQKELNQTFADLLTDVPMDQLKEWYNGYNFCGDKVYNPYDVLNYFRTKDFKNYW
                     FETGTPSFLIKLIDKRKFPIPDLENIQLTESSLNSFDIGSIELETLLFQTGYLTIESI
                     QKVGDMTLYEMVYPNKEVRMSLNDVILKYLSTLNTEKELNKVKLYRLLEAGDINGMRD
                     LFHAFFASIPYNWYTNNEIASYEGYYSSIFYTYFTAIGVEVKVEDASNKGRIDMVAIL
                     NGRCYILEFKVNEMTPNGKAIAQLKERKYHEKYIGYVSTHLGSGTVSEIYLIGVEFNK
                     TDRNITAFDWEKI"
     gene            15711..16256
                     /locus_tag="PLF66_06810"
     CDS             15711..16256
                     /locus_tag="PLF66_06810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63787.1"
                     /translation="MSVEVILLAIGCYLFCGINDKIHLMKTGDWREGGNYTNLEETIQ
                     KLTLAPNETSYEWIIEKIGKNFARQRTHRVIRKQKYNGIVYYFNKELRYINRTGHNYE
                     NSVAYVSCTNDLELNLFFRDNLLVFYNIHDSRMDRNCNESFGPLHTIQDKSWLGGGEN
                     DASSPNSCNEKFYRIFTLDKK"
     gene            16825..17769
                     /locus_tag="PLF66_06815"
     CDS             16825..17769
                     /locus_tag="PLF66_06815"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMZ63788.1"
                     /translation="MILRVENLSKTYQPFFLSKKAPLHAVKNVSFEIGKGEIYALLGP
                     NGAGKSTIIKMIAGLINPTAGKIIFNNEPVTKNTVYKSLSAVLEGTRNVYWRLNPLEN
                     LHYFANLRGISSSEVKEKATALLETLEIEGKKKNQSQHLSRGMLQKLALGVALITDPQ
                     ILLLDEPTLGLDVSSSRKIKEKIFQLSKKEGKSVLLTTHQMDLVEELADRVGIIRSGS
                     LVKEGSISDLKSIFKSYIYRIKIKGDFKLPPVWVKSYSAEIKMEQREYTEIEVDCKNK
                     QGMYKVLHELESLKKDIISFHRIEEDLEEVFLRVLSNQ"
     gene            17766..18590
                     /locus_tag="PLF66_06820"
     CDS             17766..18590
                     /locus_tag="PLF66_06820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63789.1"
                     /translation="MIRVITFIYDFLLILKAEVKRDYIFLKRYPLEIVSFIFFMYIIL
                     LAVFFGFSQLTIGSVNLLNREKMIIGYCLMQFVLSTQMGWSGQIQNESQTGTLEQLSI
                     SGHGLGEVLLARGLAQLPRQVISFYTLLFMYKMSIPDLQISFTNNFFILVTLFIMAIG
                     IFGVSYIFAGITLLFKRVGFFFQIINFAFLGLFWQDRSKLVNGSLIAHLYDNFPLTMG
                     MANLQKILINQYVELNISELNLLIFMLNSLLFAAFGYYLFHLMEQKARTLGLLSQY"
     gene            complement(18616..19290)
                     /locus_tag="PLF66_06825"
     CDS             complement(18616..19290)
                     /locus_tag="PLF66_06825"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HMZ63790.1"
                     /translation="MILFRTDEKFTNQITFTKEEIEHFRSLRINDEEKEIEIRDGKGS
                     SYFFQVGAKSKTGNLIRTETHLINSNKIGVASAIPKSNRLDFLLQKSTEIGITHFYFI
                     NFLHSERKEINLDRANKIILEACSQSKRHTIPTIEVYSSLEKFLLAEKNIFLLDPKAK
                     KNLKTNSDWSSIPIIGPEGGFRKEELDLLIQANARSFSIQENILRIETAHVFMTSLLS
                     FQSLSG"
     gene            19677..20330
                     /gene="pdxH"
                     /locus_tag="PLF66_06830"
     CDS             19677..20330
                     /gene="pdxH"
                     /locus_tag="PLF66_06830"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF004231.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HMZ63791.1"
                     /translation="MKKKDSSSISHMRRNYALGGLIETNIEKSPIDQFSIWFKEALNS
                     KILEANAMTLATADKSGLASARIVLLKSFDEKGFIFFTNYESRKGKEIQETKKGTLLF
                     FWDALERQVRIEGKLKKISTKDSDEYFHSRPFESQVGAIASSQSKVLKSREELEKKYS
                     ALLVQYKDKKVPMPKQWGGYILEPSKIEFWQGRPSRLHDRILYSKNKKGWKIERLYP"
     gene            20335..21729
                     /locus_tag="PLF66_06835"
     CDS             20335..21729
                     /locus_tag="PLF66_06835"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HMZ63792.1"
                     /translation="MPTKSYTYYDYTISLCTKCLKRISAKIIFEEEKVFMLKSCPEHG
                     TERVLIATDIEYYKRCRDFLKPGESPLHFNTETLYGCPYDCGLCPDHEQHSCLTVLEI
                     TDRCNLTCPTCYAESSPSHGRHKTLEEINTMLDIIVANEGYADVVQISGGEPTIHPQI
                     FEIIALAKTKKIRHLMLNTNGIKIAKDKEFVKKLSEFKVGFEVYLQFDSFKKESLETL
                     RGEDLREVRQNAITHLNEFNISTNLVVTVQKGVNDDEIGSIIDYALTQKCIRGVTFQP
                     IEIAGRLTQFNPATDRYTMTEIRSAILEQTDVFTEKDLIPVPCNPDALVMGYALKIDN
                     QVYPLTSMLDPEEILQNTGNTIVFERDEKLKSHLLKVFSTANSVDSITPELSTMLCCL
                     PNISAPSLTYENVFRIIIMNFIDAYNFDVRSVKRSCVHIINDTSGKIIPFETMNLFYR
                     GEKIKQLEVLRKRG"
     gene            21731..22042
                     /locus_tag="PLF66_06840"
     CDS             21731..22042
                     /locus_tag="PLF66_06840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63793.1"
                     /translation="MDESVLKKIVILNLKIYGLITLCELILVAGFIGQGDQKYGGVFF
                     MILSMFSIFLQVLTNIILSIYNYKKEDKEKAKAYLLSFIVILLVGFPSCFGFGTVMSI
                     R"
     gene            complement(22273..23070)
                     /locus_tag="PLF66_06845"
     CDS             complement(22273..23070)
                     /locus_tag="PLF66_06845"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HMZ63794.1"
                     /translation="MRILIFLLLFCFYCFTERKEEEKGIKPILKEYNIEGSTMQYAVA
                     PSNQEKLILFIHGSPGSLKAFSDYLEDKILQKKAVLISVDRYGYGGSNKGKAELSLAN
                     QAKSIAPILEKYRLPTLVIGHSYGGPVAVRLAMDYPKYISSVLLLAGSIDPELEKVLW
                     YQKLANWRLVRFILPSFLDVSNQEILPLRFELKKMEHLWKNIKIPMTLIQGDNDSLVL
                     PANVDYAQRMISDKTKLKIIRGDMNHFLPWNEYELVRKEILEMIEQN"
     gene            complement(23072..23491)
                     /locus_tag="PLF66_06850"
     CDS             complement(23072..23491)
                     /locus_tag="PLF66_06850"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HMZ63795.1"
                     /translation="MFTTKVEAVIKAPIEKVYSYVRNLETMVEYNSSLKHAKWTDSEK
                     NSCAITLSLSIVNINSDYRIISEEKGKSFLASCDSTTLKFDDYYEFEDRGENTFLRIT
                     DNIELKGLLRLSEGILSGIFRKEMQENMDRLVRNLEK"
     gene            complement(23495..24481)
                     /locus_tag="PLF66_06855"
     CDS             complement(23495..24481)
                     /locus_tag="PLF66_06855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63796.1"
                     /translation="MKNLFKLSFFIGAIFVFNGCALFQQFLGGGNEPTFTLKSVDINK
                     ITLENISLKLLTNVRNPYPVSIPKSILDMNVLIEGTNLAKFSKVDLGKIEASSNKDLP
                     VDVILKYSDLMDIYKKLPGKDLLNVKLDGVLNVPIPESYQLAGKKSFEFPFTQDKQIP
                     AVLPTIDIRNFKIVKPDTDQISSGVKDGATNTAKTYLDNLLSGKKTNLGSAAQAGLSN
                     VDIDVATEFEIALANQAAAALKFSDLKYDLALNGEKFLSGVPVDIINNGKESIVKVKT
                     SFPLKSLSSGIADAIQKKSSDFKLKGLSGLNVPGLPDGTMSFDYDKSGSFKW"
     gene            complement(24687..26822)
                     /locus_tag="PLF66_06860"
     CDS             complement(24687..26822)
                     /locus_tag="PLF66_06860"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HMZ63797.1"
                     /translation="MFKIIWNRITMYGITGEYSIETIRTMSNINRFLFLIALIHFINS
                     FVDFWNGYNLFAFLDFCISISFSILFILNRIELHKIFIHIILILFNLIIFGLSILKGV
                     HSGIFLFFFPLIIVSIFLIGINEELPLIFYSCLPILLLSISIYYNFPISFYDSDFIQG
                     ERVLFLESVFGALVLTGYILYNIVDANHEVEKRLSAEKANLNAIYNDGLLNIILFDRD
                     LKIRSFNKLANESANLLFGRNLKLDDSFLDLFLPRDQKLVIKNFEKALKGTSLKFERF
                     FKSNKHQSWYEISFSPMYKEGKIIEGVIFSSIDIAKRKEMQKNIALAKERAEAANLSK
                     SFFLSSISEEIRLAANTLLEPVDRLERKNPKKDQIENVNNLRLAIENLMIIINDILDF
                     NLLDSGNFEFDENEFDLVYLLTTIKNLAEANASEKGLEFISIFDENIPSILIGDPIRI
                     SQIAFNFIVNAIHRTNNGKICFEVKLEEVNQSYSTIGFSISDTGVGLSEESVESIFEK
                     VNPNFPIVNDGLYKSWLGLAISYKLLKFLNSAVFIETEIGKGTKINFAIQFKNSENTI
                     IKKIPDTKKDDYSDFFNGIRILLVDDYPINQIVVSEFLGKWDVDLDFASNGLEALEKI
                     KTNHYHLLLMDLQMPELDGFETTRRIRASENEDIRNVPILAITASSEAEIFEEVKEAG
                     MNDIISKPIQVDEMLEKILYQLKNQNHLS"
     gene            complement(26831..28555)
                     /locus_tag="PLF66_06865"
     CDS             complement(26831..28555)
                     /locus_tag="PLF66_06865"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HMZ63798.1"
                     /translation="MKTFRKIWNTVSELGLVKTNPDLNNQSIIYCNKIALIAALIVLL
                     NIIVFSFLNFYFYAFIYFLFFLLILPVFLLNYHFFHLAASFYLVLYSNIGTFLLCSIT
                     GKEAGNYIYFIDVLLLNFFIFSYEHKRFILYNFFITGLLMIILDITNYSLLLDSKLTI
                     FDQSKIFQISLLSTAIFMFFCIYSIIKIRNKMEIAFNEEEDTLKLILNHSPLGIVILD
                     ESRKIISYNINFKNIFIKFGIYEIKIGRELTEYIPDQEKGNLYHYLNLVEQGNIIQIE
                     KNFKTEYTSHWFDLTVSSIKTENINNRIVITLLDITDRKELEIESQIAREKAIAVNHS
                     KSHFLSTISNQIRKVMNDINISLQNLSERNPRTDQILILDKLNKSSLTLNRLIDGILD
                     YTAIESGNLQIGNSKLDLKSELLSIRDLFIDRVQEKGIDFIFIVDEDIPKYVMAEPAW
                     IRQILSHLLRNSLHFTEKGKIIFNASILKNSETKVTIRFSISDTGRGISKKTLRQLVQ
                     NFREPETQYIKNRPSFRLGFTVIKRLLALFDSHIYIESELGKGSNIYFSIEFIKMNHS
                     GIEILGGW"
     gene            28739..29818
                     /locus_tag="PLF66_06870"
     CDS             28739..29818
                     /locus_tag="PLF66_06870"
                     /inference="COORDINATES: protein motif:HMM:NF014298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HMZ63799.1"
                     /translation="MDSINNFKIKIQIQYYKILSLRYWEFWPAWFFYIPIFFYYLVFM
                     IKYRMSATAIAGVNPCFAFGGMAFDSKYDMISKFLAFPDAIAKTVLISKREIVSIIKQ
                     VKEANIDYPFICKPDKGHRGNGFKIIRSQADLENYINICARDFLIQEYIDYPLEYGVF
                     WVKYPGEEKGRITSITRKVLPYLKGDGKSKLYELILSDKRARYLAKTYLEKNQSKIDS
                     VIPKNESIRICSAGNHCQGAIFEDGMGDIKGNTKERLIEICSSISGFDFGRMDIKFKN
                     KDSLENGSGFKILEVNGSEAESTHIYDRKYSIYYAYKTLYQQWDTLFAIARKNKEKGF
                     SRLTIGALLKAYIEFFKESKEIKGD"
     gene            complement(29870..30415)
                     /locus_tag="PLF66_06875"
     CDS             complement(29870..30415)
                     /locus_tag="PLF66_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010515736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HMZ63800.1"
                     /translation="MKKFILILALTSMSMFAADFKLTSSTIKDGGTLINKHVFNSFGC
                     TGENVSPALKWENAPAGTKSFAVTVYDPDAPTGSGWWHWTLVNIPANVTELKEGASSK
                     ALPKGAVEGRTDFGKPGFGGACPPPGDKPHRYIITVYALKTDKLDVNGEASGALVGYN
                     INFATLAKTSITATFSRPAAK"
     gene            30693..31646
                     /locus_tag="PLF66_06880"
     CDS             30693..31646
                     /locus_tag="PLF66_06880"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HMZ63801.1"
                     /translation="MNILLLFLLSVLPGLFLIYRYYKKDIYKKEPWTLIWISFFIGAV
                     TVIPAGLFESWIEVPNKESMEGMILENFLVIALTEEILKLLALYFFAYKNKNFDETMD
                     GIVYGVAVSSGFATFENIFYVMQNGFTVGIIRAILSVPAHIFWGAILGYWLAKAKFHF
                     VSLFKAVLIGLSIVVIGHGFFDFVLTYNNAEYFAFSLLPVIALGLLVKRYVRLALEFD
                     LKNIHTHIELEQVENQTSVLEKKSSEAFMISLQSLISICLYFLGIIFLLTGLFLLIGF
                     IALLQENKAETWTISFALIPILAAIFMIYKAKKRKGNKRGI"
     gene            complement(31663..32004)
                     /locus_tag="PLF66_06885"
     CDS             complement(31663..32004)
                     /locus_tag="PLF66_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002180249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HMZ63802.1"
                     /translation="MVDEFKIHVDYDNGVIPIIHIAGEITSEADEEIVNCYASIPAAK
                     KTRVLIDFSKTAYINSAGIATLISLITKASENQGKIEFSGLNSHFRKVMDIVGLTDFV
                     LIHDSIEQALK"
     gene            complement(32116..32523)
                     /locus_tag="PLF66_06890"
     CDS             complement(32116..32523)
                     /locus_tag="PLF66_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016751396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HMZ63803.1"
                     /translation="MKKNIIKLILLSLVFAVAINCGKKEEKEEAKTTPEATPTATLDP
                     EVAKGKEAFDINCASCHGATGAGDGVAAASLNPKPRNYKAPASEWKNGNNEAGILKTL
                     NEGIKGSPMVAYKHLGDDTLKSITKYVVYLTKN"
     gene            complement(32597..33496)
                     /locus_tag="PLF66_06895"
     CDS             complement(32597..33496)
                     /locus_tag="PLF66_06895"
                     /inference="COORDINATES: protein motif:HMM:TIGR01777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HMZ63804.1"
                     /translation="MKIAIIGGSGLIGKHLAKSYSDHIVYIVSRKNSLPRDLANLSHV
                     KLISSDHPKAADLEGIDVIINLAGESILAGRWTEETKKRIRSSRVDKTKQLIDDLSEL
                     KTKPKLFIQGSAIGYYGMYDSGKPVFNETEKAGSDYLAKLCLDWEKEGSRANEIGIPT
                     ATIRIGIVLAKEGGALAQMLPPFKMFVGGPLGSGKQFMSWIHINDLVSAIRFIIENQQ
                     TGIFNLTAPNPCSNYEFSKTLGKVISRPSLLNVPPLAVTMLYGEGAEVILKGQNVLPE
                     KLNALKFQFQFSNLETALENLLK"
     gene            33538..34161
                     /locus_tag="PLF66_06900"
     CDS             33538..34161
                     /locus_tag="PLF66_06900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63805.1"
                     /translation="MIKYISILIFISLIYLNYSIYTTTEKKMDIYSKNPPFRLDDFTK
                     SYRKNKLSQVKNLRDQDSSTYWIKEQNSFHPEYDLETELTLTHFYKEGIFKKKNLNSI
                     TIHSCNPIADKSFLVKPNNLVIDIYLREAINVDKELRLPKDEKISSILLSFQDKTSEK
                     VDLTNILKLADSKDYPIGIYILTLFIKDNSNYPLDKKVCISEITVEE"
     gene            34158..35291
                     /locus_tag="PLF66_06905"
     CDS             34158..35291
                     /locus_tag="PLF66_06905"
                     /inference="COORDINATES: protein motif:HMM:NF017743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HMZ63806.1"
                     /translation="MSQSSTIILRSERWVNNGYSIGYKDGEAYFILGAIPEELVECKL
                     IHSSSKSREVLVVNVLEPSKDRIQSDCEIFLSCGGCSFRHIEYKLEIDLKRNLLQREL
                     IHKIPEWKDSLESINVVSGDSTGYRNNTQIKIQNEKKGFFRLGSNELVEFPKTGCKNL
                     PDEINSYIQKNNFNNKKEIKLRLTDKVFQYETVESIFTLDSLKIKVAPNGFFQINRHL
                     IVDWLNHISQFIPKEKKSILELFSGSGLISNWIANQCKELVGYEMELDAVRYATDNAR
                     INQKSNLSFIAKNLYKDRISKKHLQFPFWIVNPPRNGLGKLIIEQILEEKPESLLYSS
                     CNYVTLAQDLKKIVSYYHLEKIFLIDFFPRTAYFETILILKRI"
     gene            35368..36141
                     /locus_tag="PLF66_06910"
     CDS             35368..36141
                     /locus_tag="PLF66_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004439116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HMZ63807.1"
                     /translation="MTTNLTIENHIATITLNRPEASNAVSKELLSSFLNHLQEVFNQT
                     SLRALFIEGSGEKVFCAGADLKERASMSEKDVLHFLDQFKFTCSFLENLPIPTIAVLN
                     GDAYGGGLEIALCCDMRLMANEAKIGLTETKLGIIPGAGGTQRLSRLIGVSRAMELIF
                     TARRIEAEKAYQLGIVNSIHPRSEIQIAKSSLAEEIFSSAPIAVRMAKKSIRNGFTQD
                     LALALDFERACYLQTLKTKDRNEALAAFKEKRKPNFIGE"
     gene            36143..36661
                     /gene="dcd"
                     /locus_tag="PLF66_06915"
     CDS             36143..36661
                     /gene="dcd"
                     /locus_tag="PLF66_06915"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002770682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HMZ63808.1"
                     /translation="MILTGKEISKKLGTDIKIEPYEEKLLNPNSYNLRLFNELVVYTN
                     MPLDMKKPNPSEKIIIPEEGYLLKPGILYLGRTLEYTETHNLVPMLEGRSSIGRLGMY
                     VHVTAGFGDVGFKGFWTLEISVIHPLIIYPNVQICQIFYHTVEGEITEYKSGKYQENK
                     GIQASLLYKDFT"
     gene            36658..37629
                     /locus_tag="PLF66_06920"
     CDS             36658..37629
                     /locus_tag="PLF66_06920"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HMZ63809.1"
                     /translation="MKKKILISISVLLGLFILSFPLTRYKMNEKPHNVSIPEISQLDT
                     YLEKEESKFKDIVPGAEKKIFWANDKKEQTDYSIVYIHGFSASRPEMMPLPEMIARDL
                     KANIFFTRLSGHGRGSEPFSHVDANDWLNDAVEATEIGQKIGKKVILIGSSTGCTLAT
                     WIASQDKYKDSLSSLIFISPNFYPNNKMTNLFLYPAGLAFAKLVYGNERVWQPKNAGI
                     AKYWTHEYKWEAITPMMILVNFVQDIPMSKVVTPSLFLYTEQDKVIDFKEIEKKFQQL
                     GATQKKIKNLSQIPDHVMAGDLFSPESTEIVYKEIREFLIENNLIKK"
     gene            37633..38280
                     /locus_tag="PLF66_06925"
     CDS             37633..38280
                     /locus_tag="PLF66_06925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63810.1"
                     /translation="MNFRVVYFSLNILILFTLLYCSSGTGSNYKEVKKVDSFTGENVR
                     DNEIIISSYKEKGLISVTGSREKFIFLLPYSEDWIVKQTDNSIVQLSSNDKNLIASIS
                     TERGSFKVDPEKFLRELKNKTEIRLGMKLYDTRVLSAPTNKILGYYIKLENKGLEIKS
                     DNFWSIRQRPDDVILKLHLSMLNVSEEEIRKAEKILPIFMDNGFRILSEEDLEKL"
     gene            complement(38275..39285)
                     /locus_tag="PLF66_06930"
     CDS             complement(38275..39285)
                     /locus_tag="PLF66_06930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63811.1"
                     /translation="MKSFWKSNINKISLLISILCFQFLFIQYFKYLNYIPDHSFKRNK
                     NAIWIGHKWVGEYLSDEDYSLLCAKLQTGKITDVFAHVGPLASDGSIPQEKYPYAREF
                     LNRMRDCSESIHIQAWIGQLEKKGGGKLDITQSSIRESIVLTAESFLNLGFDGIHYNI
                     EPIYSGDESLLDILKKTHTITKKQDKILSMATDEIEPFPFADKFLRFFSSRAGLWTSD
                     FYIQVANNVDQISVMMYDTGTPLSWVYAYIVKRQVIKITSILEGKVTLFFGVPTYEEE
                     RWSFHSEAENIFSGIRGVSLGIDSLPIDLVQDKFGIAIYAEWTTDDTEWKIFRREWLL
                     SL"
     gene            complement(39282..39926)
                     /locus_tag="PLF66_06935"
     CDS             complement(39282..39926)
                     /locus_tag="PLF66_06935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63812.1"
                     /translation="MKKIRWLFIPLLFSLSNCVSIQNYLSNRAFDLSDIVTIGGEANT
                     YGASAWLWCFGGGLQIAQDGKGLGMRNGYIGSYRTGGNRKLGKLKMGNSFFLMNSQEH
                     IPEAYKTSRSMRKKYTHANFLFILPASLQGRWRSNQYYTNPNNYNNNYDFINPSANLP
                     NLCHAPVNVEFSLGLGIGARIGINFSEALDFILGLTTYDLMEDDALNKEETEEE"
     gene            complement(39936..40514)
                     /locus_tag="PLF66_06940"
     CDS             complement(39936..40514)
                     /locus_tag="PLF66_06940"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HMZ63813.1"
                     /translation="MAKRISSKEDNPKERILETASKLFYLQGYSNTGINQILEESKTF
                     KLSFYNYFSSKENLGIEYIKIRETEFLAFMERLMKNNPTYESFVRTWIRFLRVEIKNR
                     KYHGCPFINITLQTFGKEEVFKPHIKSMIQKWKKSITNFLIRSQAANKAQAEKMAVLI
                     IQIFEGSVQLYMATDNIDYIYELEENLVSILN"
     gene            40785..42113
                     /locus_tag="PLF66_06945"
     CDS             40785..42113
                     /locus_tag="PLF66_06945"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002103311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HMZ63814.1"
                     /translation="MKSPKQIALCSPRRIPFAQIGKSLSNYSAHHLGKIVADDIIEKT
                     KLPLNKIDGVIVGEGFANAPNSARVIANLIGLPNEIPCLTVANNCVSGMEAVVESARR
                     ILLGEGELFLAIGEESQTSMPFVVKNARINKKTASLDKLKKALSESNLPEGVEVRDTL
                     EDGLGDGETSYGMQVTAEIVSQNYALSRAINDKVAFESFKRALEATEAGLYDPYIIPV
                     KDDEGNMMKADEAVLLRKGLVENPDRMGRAMLLFDNPQMKFTEFQTKYAKDLKKTHAP
                     TVSIFNASPRSDGAAGVIVTTVEKAKELGLQIDAVLTGWKMKGVHPNEMGLGQAAATL
                     GLLQDMNVKIQDVDRVEIHEAFAATAVGALEEIKNQTGWDWEKSFDEKKINTYGGSIS
                     IGHPFGATGIRLVANAVMDIKNDSSVNKVLITACAHGGIAGALMIERYKG"
     gene            42241..42732
                     /locus_tag="PLF66_06950"
     CDS             42241..42732
                     /locus_tag="PLF66_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004787528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4442 domain-containing protein"
                     /protein_id="HMZ63815.1"
                     /translation="MKKNPFEQKKRWLPKYFKVTYFRLLLNFYFPYLGAGITVTHISK
                     DFRRFDVKMRLRGYNRNFVQTHFGGSLYAMADPFYMFILMENLGENYLVWDKAASIEF
                     VKPGTGTVYATFEVSKEEIESIKQEVDIQGKLNKIFHAEIKLENGEVISRVEKTLYIR
                     KLK"
     gene            complement(42813..44321)
                     /locus_tag="PLF66_06955"
     CDS             complement(42813..44321)
                     /locus_tag="PLF66_06955"
                     /inference="COORDINATES: protein motif:HMM:NF021778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HMZ63816.1"
                     /translation="MSRIETIVNFFKFLNHLKLSLFISSLLLLFLCANCNRTKYSNPC
                     DFNSAFFQRAFLSKIILGDKTPLCGIPTTTNNSNPNTSITPITSFTYTQSIYTMTQGV
                     SISPIIPSLTGTITRCATTPSLPAGLIIDNATCTITGTPTVGTTLTDYSITATNALGS
                     MSTNLKIRSLFGVGKFIYIPNFSSNNISIFSIDPNTGNLTTGGTVGFGTGTRDVAVDP
                     SGKYLYAVGNSSNNIQLFLINNTTGALTASGSPLSTGTNPNSVTVDPTGKFVYVANGA
                     GTISIYKADTATGLLTSINSIPSGTTTRWIRVDPYGKFAYAVNGGSNDIYIYSIDSAS
                     GNLTQVGSPLATGNDARSVTITPDGKYLYVANFNVNQVTPYSINQVTGTLTVGSFLTF
                     ANSPESVFVHPNGKYLYVAYTATGPLGTVASNPINSLTGAISTGILATNGIVSPNRVF
                     VDPSGKFAYSSSYNSNSITVYTIDPNSGNITLNTSYTPGTNPNSLFVTGANP"
     gene            complement(44635..44719)
                     /locus_tag="PLF66_06960"
     tRNA            complement(44635..44719)
                     /locus_tag="PLF66_06960"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(44683..44685),aa:Tyr,seq:gta)
     gene            complement(44757..44829)
                     /locus_tag="PLF66_06965"
     tRNA            complement(44757..44829)
                     /locus_tag="PLF66_06965"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(44794..44796),aa:Thr,seq:tgt)
     gene            complement(44955..46940)
                     /gene="uvrB"
                     /locus_tag="PLF66_06970"
     CDS             complement(44955..46940)
                     /gene="uvrB"
                     /locus_tag="PLF66_06970"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004431423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HMZ63817.1"
                     /translation="MPDFQLVTPYKAAGDQVKAIQKISDSFLKGKKKVTLVGVTGSGK
                     TFTMAEVIQKVKRPTLVLSHNKTLAAQLYREFKEFFPNNSVEYFVSYYDYYQPEAYVP
                     SSDTFIEKDSSINEDIDKLRLRATSSVLEREDVIIVSSVSCIYGLGSPEDYTESRIIL
                     KRGQTLERDLLIKKLLHIQYQRNDLDFSRGNFRVRGDSLELMPAYHEDGLRIEFFGDE
                     IESLSRFNPLTGKVISKMETMVIYPAKHFITSGPKVEDAIRNIKEEVAIQIKSFKEKG
                     KLLEAERIESRTNYDLEMLAELGYCNGIENYSRHLTGRNEGERPACLIDYFPKDFLLI
                     VDESHVTIPQIGGMYAGDRARKETLVEFGFRLPSALDNRPLNFQEFETMTDKILYVSA
                     TPADYEIKNSEIQVEQIIRPTGLLDPVVEVRPVANQVEDLLIEINARVEKKERILITT
                     LTKKMSEDLTDYLKEVGVKVAYLHSEVETIQRVEIIRDLRKGIYDCIVGINLLREGLD
                     IPEVSLVAILDADKEGFLRNYKSLIQTIGRAARNINGMAILYADKLTDSMKKAIEETN
                     RRRKIQSEHNQKFGIEPKTIIREIHDILPREVAELSAEEETINEIEREFTLKKYKTPE
                     KLREKLKEAMLAAAKELNFEKATMLRDKMLSIQDKNK"
     gene            complement(46945..47613)
                     /locus_tag="PLF66_06975"
     CDS             complement(46945..47613)
                     /locus_tag="PLF66_06975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HMZ63818.1"
                     /translation="MPLEITDILLEKLIRDILAEGLTGTVKDYCSWLSLEGVLKYNEE
                     ISVEESKVKAILDSLKESGFAREGKFDKSEYCITPNAIPKRTDYLSDSYSVYGDAKFS
                     PIQYVRSRQEYFLEYNYVTEEDIMDISIATIEAIENAVKYGDGNIVHIDYMIDSNRVL
                     KLNIINNIKEFDLTNEIERGKYSSNTTLMRGIMVMQKLFNHLELQIIDDKKQAHLYAE
                     KKLS"
     gene            complement(47806..48279)
                     /locus_tag="PLF66_06980"
     CDS             complement(47806..48279)
                     /locus_tag="PLF66_06980"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004753884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HMZ63819.1"
                     /translation="MVLILNIVLFRPEIPPNTGNIARLCVGIKANLHIVGGASFDLSE
                     KAVRRAGLDYWEHLTLYQHKDWESFIQVIPDLSRVFLVTKFGESLYSSKTYQKDDFFV
                     FGRETTGLPIEIRNAFPADNQIYIPMAKECRSLNLSNAVAVIAYEAVRQLNLDFN"
     gene            complement(48283..49422)
                     /locus_tag="PLF66_06985"
     CDS             complement(48283..49422)
                     /locus_tag="PLF66_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002975161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HMZ63820.1"
                     /translation="MANKNDFATLLEESFKKRKSLEPGAKYNAIVSSVKSDYTFIRTE
                     PDSIRGIISNAEFIEGENKLEPGKTYSVYFLKENHGDYYFTTVLTGEDITPENLEIAL
                     EHEIPVYGQVGGETTGGYDVKIGEYNAFCPYSQLDPETKSQALQGKKLKFIVNDLSMK
                     NKKIVLSQKKISDKEKDLKREILKGELKEGSYVTCKVKSIHNFGLIVDMNGIDALVPV
                     SEASFKKNVDLNTEFTVGATVRGKIIGMDWKENKFSISLKDSSNDPWAKAVPFKEGDI
                     VKATVDSIKNFGVFVKLNEHFHALLPTKETGYPPRTSLSNHFKAGDILEVFITEVNPE
                     KKQIAVSLSKARDAKDRIDYENYISEQKSTSESSFGLLLKKSLKK"
     gene            complement(49437..50642)
                     /locus_tag="PLF66_06990"
     CDS             complement(49437..50642)
                     /locus_tag="PLF66_06990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63821.1"
                     /translation="MNKNILYGVIGILVVAVGLFLFLGNGKKSSKGKSGDPMSQSSDP
                     TKSGAENFEDKTTTTSESGPGKISDGVTPAFLLEQYTEWAQYPPHSRPISSLNQDIIH
                     PFKIDINAITMVDSPNDKEGNGYRCHLQPKTWAVIGSNSDMIIKLECRDSSNDLIKVN
                     VVNATVYKEFEDQKTPTHSPDFNDDGRDGDEVAKDGIVTFKWKPMKADWGQMSLEANI
                     TYAKDKKAKLTTSFFSSPNKPAEIGNTFRDTLSDGSLVIHAPVIVYKAGNYRLEANLK
                     EEKEGNYLAYATFDGPLKQGTNDVEFLFFGKILRDKGYDGPYVMTNVRGYRVNLPIDP
                     QWLEQGEEGLKKIQAAKTTEPDKELIVPFMEEYKTKAYTASQFSKKVWESDDKNKRIQ
                     ELQQLAQQK"
     gene            50793..51611
                     /locus_tag="PLF66_06995"
     CDS             50793..51611
                     /locus_tag="PLF66_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010576001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63822.1"
                     /translation="MLKSKINLLVVAVSLAVSASLSAGTITVFVHGKSNSNHNGTGTT
                     DVNNYWGASANTVSGVKYFVGYDGTSDPRTYGTARAQTNLTTVLVNQCKGTNTCKIVC
                     HSAGCYATEYWLSNLGGTASSKGFKISGVTALAAASGGSELANSLNAISFGYVGNAMD
                     KALIVTNARSSFNHNNTAGVTVAHVPGSKGMIGASAILPGEDDYAVAYHSSCGYSTVG
                     GLSKCQSSITQSEGIWPFNSNVTYTQYLGHTRAPSVPVEGLYENHSELTNDGWR"
     gene            51849..52667
                     /locus_tag="PLF66_07000"
     CDS             51849..52667
                     /locus_tag="PLF66_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008595504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63823.1"
                     /translation="MLKSKIKLSLLVASLFLSASLAADTINIFVHGKSSSNHDGTGTT
                     DVNNYWGTSPNSVSGVKYFVGYDGTTDPRTYGPARAQTNLTTVLVNQCKGTNTCKIIC
                     HSAGCYATEYWLSNLGGTASSKGFKISGVTALAAASGGSELANSLNAISFGYVGNAMD
                     KALIVTNARSSFNHNNTAGVTVAHVPGSKGMIGASAILPGEDDYAVAYHSSCGYSTVG
                     GLSKCQSSITQSEGIWPFNSNVTYTQYLGHTRAPSVPVEGLYENHSELTNDGWR"
     gene            complement(52939..>53120)
                     /locus_tag="PLF66_07005"
     CDS             complement(52939..>53120)
                     /locus_tag="PLF66_07005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63824.1"
                     /translation="ATTNKFIFDFNIFLFSYLEFLFEIHTANVKKAISDYQSKNKLEE
                     KKALQYKIEIQFYLC"
BASE COUNT        17227 a         8808 c         9272 g        17813 t
ORIGIN      
        1 catttatttt taaaggctga cttttaattc tttaacattt cgcaacagac tgataaaata
       61 aaagatacga atttaagaat tgttttgcct gtaaaagaat cctattgact cagaaaccta
      121 attgctcatc actatgattc cgttgatatc cctactatat ctgagattat taaatttgat
      181 attcctgatt taagggaaaa attaataaag cttgtaaatg aaaactaaaa aagaacttct
      241 aaatagaatt tctataaatc ccaatatatg tttcggaaaa ctttggatta tgcaacaaaa
      301 tggcattcga gaataccaag aatagtaaat tgcattcatt ggaataaaaa tttaatacta
      361 tgcataacgg ttgcaccctt cgcaacagaa tccaagacta ccgtaggaat gggtgcaaat
      421 gctataatct caagtcttcg ccaattcttc caatgcttgt tttgcttggt ctgcgttagg
      481 cggagttccg tgccattctg gtttgttttc cataaaagaa acaccttttc ctaaaatggt
      541 tctaaatacg atgagagtag gagaaccttt gtgggactgt gctttttcaa aagaagaaag
      601 aatttcatct aggttgtgac catctgcttc taaaacattc cagccgaagg tgcgaaactt
      661 ttgaggaaga ggttcgagac gcataacgtc ttctgtgttt ccatcgatct ggatgaaatt
      721 acgatccata aatgcaatta gattgtctag tttgtagtga acagctgact gtgctgcttc
      781 ccaagtcatt ccctctccac attcaccatc ggaaatacaa gcatagactc gatttgtttt
      841 cttttggtga cgaagtccga gagcaattcc agttgcaatg gaaagacctt gtccgagaga
      901 accactggat gtttcaagac catctaagta ggtaatagaa ggatgacctt gtaatctact
      961 tttgatattt ctgaaagttg caagttctgc tacaggaaaa taaccactga gagccattgc
     1021 cgcataacgc acagcgcaaa catgtccatt ggataaaatg agtctatctc tttcttccca
     1081 gtttggattt ttaggatcgt gttttaaaat tttaaaatat aaagcagagt aaatatctgc
     1141 aagtccaagt ggaccgccag gatgacctga ttttgcggca gtcaccattt tgattacttc
     1201 ttctctgatt tctcttgctt tggtttttaa ctcatttgtt tgcatgttaa ttccttgttt
     1261 caaaaataaa aagtcctgat ataaatacga tagtgtatag aggtaaaaaa ataaaatgaa
     1321 tctttttgtg taacttggca ttctttgtga ttccagtgta tcccataagt agcatcaaaa
     1381 ttaaactaag gaaagcaaag gctctgtgga tatttacaat cgtaataggc acacttggaa
     1441 aaattccagc tgcctcaatt ccattgatta aatatttatg gattaataaa aatactgcag
     1501 tcgcgaggtt aaataaaata ccaagtaaat tgattctttt atgaagagta ttgtttgatc
     1561 ttctaaaata atatccaata taaaaacaga gaatagaaag agtcattcct gcattgatta
     1621 gatataggtt catgtatgcc atgaaatcag atcaattcct agtttcaaat agattatcat
     1681 ggcaatccta ttttcttttt agttttccaa ggatctttcc aaagataaga tcaaattcat
     1741 gtagctttgc aatttttgaa aaagaaaaat agagcacaat agcgggaaga attccaacca
     1801 ctgccttgag tcgcgctata ttttcatgag ctaaattcat ttcttgtata aatgaaaaac
     1861 tttctaaatg taaaaaattc tctgtaaaga ttagccaaag agttaaagcc gttagtggaa
     1921 ttagaatttt taaaagacga aaaagaattt ctttttctgg aattctaatt tcatgctttc
     1981 tcattttgaa aaaaagaaag gaaaaagtaa tgtgtgctgc aatagtagaa gaaagagcaa
     2041 gccctgcatg ctttaaaaaa ttcattaaga ttaaattgag accaatgtta atactaaacg
     2101 aaatcatatt cacttgcaga ggagtttttg tatctttaaa tgcataatag gaagaagtta
     2161 aaatcttatt catgctaaag actggtatgg caagagaata gaactgaagt gcttgaatgg
     2221 tagaaagagt tgctttacta tcccactttc caccataaaa aatagcatct acaattgtct
     2281 ctcccataaa cacaaatcca aacgccgctg gaagcgttag gtagagcgca aaacaaagag
     2341 tttctgccag ttcacttggg atagcggctt ttttatcttc tttgaggctc gcgagaagtc
     2401 ctggtagagt agtggtggca agtgctactc caataattcc tgttgggagt tgaatgagtc
     2461 tttgggaata gtcaagactc acaacggctc caagacctgg gttttgattt tggatgtaat
     2521 tggctaagaa gatatcaacc aaaagactga tttgatagaa tccacctccg atcacagctg
     2581 gaatcatgag agtaataatt tttttaatcg cgggatgttt ccagttaaaa ttgatcttgg
     2641 gaaatagatt tagttttttt acatagtagg cttgaacggc taattgtaag aaaccgccag
     2701 tcacaattcc aaaagataga actttaactc tctccaaagt gtcttcgtaa aatcgaaaga
     2761 ctacaagaaa tacaaagagg taacttaaat ttaaaataat tggagaaaga gaaggaacaa
     2821 aaaatttctg tttagagttt gaaatggcaa tgtaaataga agatagactt gctgtgaaga
     2881 ttaatataaa aagtataagc gataattcta ttactaaatc agaatattct tttgttcctc
     2941 caacgagtaa cggaagtaaa taaggagcaa ataaaacaaa tacccctaca aacaccaaaa
     3001 gcataaaaca aagaaatgaa agaacggctc ctgacatgag agaggcttct tcttcgctta
     3061 ccttacttgc atctgaatac aaaggcataa aagactgaga gagggttcct tccgcaagta
     3121 gatttcgata catattagga agacgatagg cgactgaaaa ggcagaggct acgaaacctg
     3181 ttccaaaact aatcgccata taatgatcgc ggataagacc gagtattcga gacagaaagg
     3241 tataaaaaga taatgcgata cttttacttg ctgagctaga tgtttttttt tctacttcca
     3301 cttgattttt acttttggtt cccaatttag tttttccatt actttcagta agtccgacca
     3361 aacatcctgt tggactttgc tgttttctcc cccatttcga attacataac ttggatgata
     3421 ggtaggcatg acaggaattc ctttatactc tgcccatttc cctctgagtt tagtaatacc
     3481 ttcttgtgtg ttcaaaagaa atttagtaga aggatttccg agagtgacga tgacttttgg
     3541 ttggatgatt tctatttgct tcaatagata aggactacaa gcagctactt cttctggatc
     3601 gggtgctctg tccttttcca tctttaagtc ttccgtaggt ctacattttg cgatattcgc
     3661 tatatataca cttgatctag gaactcccat cgctttttca atgagccgag tcagtaactc
     3721 tcccgctttt cctacaaagg gtcttccggt gatatcttcc tgtttaccgg gaccctctcc
     3781 aataaaaagt aattcagctt ctgggttgcc ttctccaaaa actactttgg tgcgggttgt
     3841 ggataacttg catttgacgc aagcttctgt aattttttta agttcttcta atttttctgc
     3901 tttcaatgat tcaaccatta tccggttgtt ctgagaccac ttgcaattgc attaatagag
     3961 agaagcaatt caatgagtag cttttcggca gagccggaat tttgatcctg taagttttgt
     4021 ctccattgtt tgagaaggtt aatctgcatc aagtgtaaac tctttagtcc agaacttcta
     4081 aaagcaagag tcctaaacat tctaggtctt ctatcttcta ctttagattc aaataataga
     4141 gaaagcattt ctttggttct gtcatattca tttacaattt gtgttaaata tttatctcga
     4201 acctgatcat catccacaag atgaccataa aattgcataa tctctttgtc tgcattgtaa
     4261 atattggttt ctacattcat cattagatag cggaagaaag gccagcttac aatttgcttt
     4321 tttagaaatt gaaatacatc tggttgatgg atttgaatat cactcagtgc agttcctaca
     4381 ccaaaccaac ctggtagaaa gaatctagat tgattccagc taaacaccca tgggatcgct
     4441 cttagatctt ttaaagatct tttccctgtt cttctggcag gtctagaacc aatacgacta
     4501 ttttctaacg cgtcaattgg agttgcttgg ctataaaaaa tcatgaagcc ttcagagcga
     4561 actaagtttt cgtaggctcg acgacttttt tcagcaagac tatgaacaac tttttcttgc
     4621 tcttctgtaa tctttccaat ttttttatgg gactggctaa cacctgttac ccccgcaacg
     4681 agaagttcta gattataggt tgcgttgatt agatttgcaa attgttgcgc aatgctttct
     4741 ccttgaatgg tggatcgaat ttgaccagtg agagtccctt cgggaagtgc atctagaaaa
     4801 tgatttgtct ttccgcctcc gcggctaatg gttccccctc tgccgtgaaa gaattttatt
     4861 ttaacaccat gtcgcctacc gacctcatct agttccaatt gtgctttgtg taaataccat
     4921 tgacttgcta taattccacc gtctttatta ctatcactgt agcctaacat gacttgttgg
     4981 gttggaattc catctggaaa ggattgatag gcaagactta cacgagtcat tggatgagcc
     5041 aaaaatgaat ccataatcgt tgcacaattt tccaaatctt caattgtctc aaagagtgga
     5101 acaactggca ataaacaaac aagcccttct gttgtattca ttacaagtcc cgcttccctt
     5161 gcaaatagat agaccacaag caaatcggaa actttacggg tcatgcttac gattaacgca
     5221 ccgattccat gtcttccata ttttttcaca tattcagaaa tcactaaata acaatctctg
     5281 actgcttgtg cttctccttc gagtggcatc ccgggagcaa gaaagggtct tgcggattct
     5341 aattcctcat ttaaaaaatg aagtctttcg aattcattcc agtctgcaaa gtaaatgttt
     5401 ttacttccag aggctttcat taaaccagat agtgctttat catgaaactc actgttttgt
     5461 ctaatatcta aagccgcgag gtgaaatcca aaactttgta tgagtctttc taaggtaaaa
     5521 acatcgtatt ctacaattct aaatgcatct acttctaata gagaatctct gagtattgaa
     5581 atatctttta gtagatcaaa atggtttttg taactatatt ctttttcttc tacagatgga
     5641 aatggaattc ttgctattaa tatgtttaca aattgtctcc aaggttcatt tgggttacgg
     5701 ctaatacatc tttgccccac atctcccatt tgctctaccc aatcttcaat aaattgattg
     5761 agtcttcttg gaatgctatg aaggttagcc gaaagactca atttggctgt gaggtctgtg
     5821 agtcttttat gaatgatatt taatgcggtt tgtcttagtt cgtgaagtgt ttcctttgta
     5881 acttctgctg taactaaagg atgcccatct ctatctcctc caacccaatt cccaaaacta
     5941 attttgggaa gtttctcgat tcgattgata agagttggat cgaaatgatt tgcaagccaa
     6001 gcttgtttta attttttatc ttgtaaagca attgcatgag gaaaaacatt ttctaggtag
     6061 tggataatat tttttctttc tgaattgatg tctggttttt gcaaatagat ttcaccagtt
     6121 cgccaaagtc gctctagaca aagtttgatt tcatttctca aattttcttt ttcagcaggg
     6181 gtccacattg agttttctaa ttttactaaa agtagataga gttctctgtg ttgatttaat
     6241 acagtcgctc ttttggcttc tgtgggatga gcggttaaca ctggttcaac gtggatttct
     6301 gcaagagatt tggaaatggc agtgctatca aatcctaagt ttttgagttt tcggatattt
     6361 tgtccccacg atccaacaat ttgatcgata cctttttcga tttcaatgga tcttctaaat
     6421 tgagtagccg cattttcttc taccatgttt aaaatttgaa aggaaataga taaagcctgc
     6481 gaggctcgga gcggaaaatt tgaatctatt ttttcgaaat aatggggagt tctatctcca
     6541 tctacccaag gaagattttt tgcaatttca tcttctttta actcgattag aacttcttta
     6601 aagcaattca ttaaaaaaat taaatcattc ttaaccttac tagattgatt ttcctctgcc
     6661 atggcttctc tctactttta aattgattta cttttcaatt acctaaaata ctgatttttg
     6721 taaattagat aatctcaaag ataaaataac taaatgactg atctgactca aaattaaaag
     6781 ttttagggaa gaggatggct ttaagaatat tagagaatcc taatgaaata ataaaatcaa
     6841 taccctgcca taacttggct ctcgtaggct caaaaaggga ggaatacaag cccaatgttt
     6901 tttctcattc gctctaatta agtccaatcc cattttcaat tccaagtaga aaacggttag
     6961 gttcaataaa gctcatttat tgctcttgtt cagaatgact ctttaagttg aggactgatt
     7021 caccttctat ttttataagt cttagattga aaaaagaata ttgtaagcac ttcaggtgag
     7081 ttacttaaaa aaaacttgac atccttgtaa aaaagaagac ttatcatggt ttaatcaaac
     7141 cataggttta ttattagttg aatttttcga ctgacaattg ccgatatttg tggaagggaa
     7201 gaattataaa tggcaaaaaa gaaatctgga acatccagca aaaatacatc tccaaatgta
     7261 gaagcagtag aggaaacttc atctctacct gatccggcgg caccaccacc tacatctaca
     7321 ccagtaacaa gccatcacaa tcacttggca agaggtcttt tattaatact tgcttcctct
     7381 ttattattgt ctttccaaaa tgtggttaca cgcgttatct taagtccaaa gtcactcttt
     7441 ggattgtttg aaggaatcgt tttaaattcg agtttatcta actcactttt gattctagtt
     7501 ttacgatgtg cgattgttct accaatcatg gcttttattg tggctccatg gcttcataaa
     7561 aatacatggc aagatattaa ggatttacct aagcctgaaa ataaaacaag attatacagt
     7621 gttttaagta gtggtttgtt tttatttttc tctcagcttt gtatgtatat tgcccttgga
     7681 acgattccaa ctggaattgc taccacgata ttttttattt atccaacgat taccattctt
     7741 ctcatgtgga tcttttttgc ggataaacca tctttacctc taatcttcgc aatggttacc
     7801 atttatattg gtggatttat gaccattcca atgactgcat tcactccaaa aggagagggt
     7861 aactttgtgt taggtgcggc aacagcagct ctatctggaa ttgcatttgc tggttatatt
     7921 attatgatta aaaatgcaag aatgcatcct gctcctttta ctattgtgaa tttttctatt
     7981 attctcattt tagggggaat gattcttcca tttatggatt acagcattga ttcaagccat
     8041 atcatggatt tggtctatgg aaccttgatt ttagcaacaa ccactctact cggatattta
     8101 ttacaaaatt ttggtgttcc tttggttgga ccctctcttg cctctgtgat tggggcgagt
     8161 ggaccggcag tgactacaat catggcactt gtattgattg atgagacatt gaatctgcaa
     8221 caggttttag gtgtgttcct tgtaactttg tgggttttag gaatcagtgt tgaaaacatg
     8281 aaaaaggcac cagttccgca aaaaaaataa atccagtatt ggttaaaacc atccgccttg
     8341 gctctgcggc tgtttgttta aaaaaaaatt acaagatttt cacagataaa cactgataaa
     8401 agattaaaat ttttaaataa atcagtgtct attcgtgtta atccatgccg aaaagccctt
     8461 atcttaatga caataggggt ggctgtccga taattaattt aacgttaatt tttgagttat
     8521 ttgggaatag agatgaagaa taaaatttta gtaattcttt tgtatttaat agtcattggg
     8581 ttctatgcag agtcctctac aaaaatagat aaatcagaaa tgaatcgaat cgttctttgg
     8641 gaaggggaga ttactggaat ctataaagat aagggttctg ttagagcttt gatctctcat
     8701 aatccagaat ggctaggtgt taattttgaa gagattaaga atgggattct aaagaaaaag
     8761 aaatacgaac tcagacaaaa agttacgaat aaaaagattg gttactttat tgcagatcat
     8821 gtagatctgg aaaggaaaat tgaaaagaaa caaaaaacag actaccaggt tttaattcat
     8881 ggtaagtttg atttggcaga tccttcctat caaaattttg tatccaatga ttttatcatc
     8941 gcaacagttc gatatgaaga tagttactta gatccatctc gtttttataa agataatatc
     9001 acttcaccaa aacaaagtat cattcaccct gtagatagaa aggaaatggt tttggttcct
     9061 gcgagtgcgt ttatacatgg gcaaggggca agtggtgaac tagatagcta caacccagcc
     9121 tttcaaaatc caaatgacac aaatctactc gaaatttctt ctttttatat tgataaatat
     9181 gaagtaacaa atggcgaata tgcacgttat cttcgtgata caaatgcgaa tcctccactc
     9241 tactgggtgg atgggaaaat tccttctgga aaagaagatc atcccgtgat tgcactttct
     9301 tatagagaag tagagcgata tgcggcatgg gcgggaaaga gacttccgac agagcttgaa
     9361 tgggagaaag cggcaagagg acaaggattt actagaactc taaacagaga tgagtcttat
     9421 acaattcaac tccatacagt tatctaccct tttggaaata aatttgactc tctactttgt
     9481 aatacaaaag aaagtaaaat tggcgataca gtttctgttt acgaacttgc agtcaaaggt
     9541 gcaagtcctt atggagcgat tggaatgtgc ggaaatgctg ctgaatggac tagtagttgg
     9601 tatatgccgt atgacggtca ttatcttaag aattcatcat ttggaaaaca atacaaagtg
     9661 attcgcggcg gctcattcta tgaggataaa aattcggcaa aggtatatac gaggtctttt
     9721 ggtggaaatc caaatcttgc tgaagaccgc cgagctggat ttcgattagt aaaggatttg
     9781 aattaaatgc ctataccaag cattacaggc tggaaagctc tcaagaaatc atctcaatta
     9841 aaagaagata cacttggttt ctttgtatct caatttgaag aatacggaga tttatttcat
     9901 tttaatctac ttggattaaa catgtttata atgagagatc caattctcat tcgatatgtg
     9961 ttacaagaga acaatggaaa ttatacaaaa agcatttttt ataaagaatt ggagagactc
    10021 ataggaaaag gtcttttaac aagtgaaggt tctgagtgga aaaagaatcg tagactcact
    10081 caaagtgctt ttaagaaaag ttctatcgaa ggatttagtc aaatctttta tgaagagtct
    10141 gatcggttag ttggggaatg gaaagatata tcagaagtag atatgtcaaa agagatgatg
    10201 cgattaacgt ttcgtatcgt aggtagatct ctttttagtg ccaaattaga tgaagatact
    10261 cagatagtag atgaatctct tggtattgcc ctcgaggaaa taactagaag aattcaatcg
    10321 ccaattaaga ttccatttat actcccaaca cctagaaata gaagattaaa gaaagcagtt
    10381 cataatttgg acagtgtggt aaataagatc atatccaatc gaatcaagtc gggcgagaga
    10441 gtaaaagact tattagatac attgatctat gcaagggatg aagaaacaca gcaaggtcta
    10501 agcccaaaac agattcgtga tgaagtaata acttttctat ctgcaggaca tgaaactaca
    10561 tcgaatgcac tcacatggac tttctattta ttgtccgagc attcggaagt aagaaaaaaa
    10621 gtaattgagg aaattcgatc caatattcct agcacggggc aaattactat tagccagtta
    10681 gagaaattaa aattaactga aagagtctta aaagaatcaa tgcgcctttt tccgcctgcc
    10741 tggattattg aaagaatgag tataaaagag gatacaatcg gggaaacaaa aataggagca
    10801 aactcacttg tttcagtttg cacttatgca gttcatcgca atccaaagta ttggaataat
    10861 cctgaattat ttgatccaga tcgtttttta ccggaagagg aagaaaagcg tcacaatttt
    10921 gcttatattc cttttggtgg gggtcctaga gtttgtattg gaaataattt tgcagtcaca
    10981 gaagcgatga ccgcacttgc ctgtatttta agaagctatg aaccaactct tattgcaggt
    11041 caccctgtag taaaagaacc gctagtaact cttcgcccaa aatttggaat gaagatgcat
    11101 ttaaataaac gctaattcaa aaggaaataa aaagattacc accgacttgc actgagccat
    11161 gtcgaatgtg taaacaccga ggacaccgat aaacagtatg gcaaatcaaa atgatccatc
    11221 gtttctttat cgtttagtta tcggagtcat cggtggtagt cttttaaata tttcttctat
    11281 tgaattgatg ccatttaaag cattagttgt cttcagggag tattgaattt agattctctc
    11341 ttgtccattt ctctaataga aaaacgccaa tggctaagag aggaagaaga aaacttccaa
    11401 aaatagcaaa tccagcagag cctagtagaa aaatagagga gattgtttct aaaaatccaa
    11461 ttgatttaga ttcatctgtt aaaactaaaa tacaagaaaa gagaagggct attaaataaa
    11521 ttgaaattaa tcccattccc atcaatttaa aaaatcttct ctgttggata aacttagaaa
    11581 aaaaaccaag caggactcct acaactggaa aggctggaag gataaagagg actgcgccta
    11641 attcctgtaa gccgctcact ccaaaaaaga gaatatatcc cagaaataga atcagtagaa
    11701 aagaaaaaga aatgataaac ccttttagag tggtagtcat tctttaatcc gttttttctt
    11761 ggagtaaata aagatctact gctgtatcag ctttgttttt attgagtgca attggtccac
    11821 gttttacaaa tctgtatctt tctttgagta gttcgaaggt agcggaagta acttgaattt
    11881 ctccattgac tccatgtttc tccattgcat ccgcatcttt aaaggaatca tttatataga
    11941 aaatatcaat tttttgtgca tttgtgagat agtctacata cttaccactt gccattccca
    12001 ttcgcaaatc tagtttgagt ttattttcac tgcaaatgat ttgaaatttc ttctgtagat
    12061 ggagagctaa atcggctaac ggatctgcat agagtggatt ggttccggct gggtgatcaa
    12121 ttacaagata ccaatacttt ccatcttctt catgttcttc tattttattt tcgatacaga
    12181 aatcttttgc gttggaaaag agttgtttta agaattcatc taatccgatt aaggtttctt
    12241 ttgctctaaa ataatttgca attccagtaa tatcaaaata aagtagggtg atattttgta
    12301 gatagtaagt gacaggcagt agtcctggct gtcccaattt aggagctggt aaaatctgtc
    12361 tgagtaagtt ctccgcataa tttctccaga aatctgcgcg atcgatgagt ctgatttgat
    12421 tcttaatgta tttatctaca aaataagtca ttcctaaacc gactgttgga actaacgcta
    12481 aaatagagca ttgaaataag aaaaaataaa tgtcttcatt gctagtaaaa acagacttgt
    12541 gaagtagaag taggaaggaa tagactaaga tataaacagc cgatacaact aacgcattta
    12601 aggttttcag tcggagcccc gcagtgatta acagtaagta aaccgcatac tgcaatagat
    12661 aataataggt ctcttttaca atcggaacgt tttgttccaa gagccactct ctagatccaa
    12721 tgataaggaa taaattcaca ggaattccaa tcacaatggc tagaaaggtt tttacttgga
    12781 catcttccgc cttagagata tttagcggct ttaaaaaagg atgaagtaga attacgacaa
    12841 agatctttag tgcatttaaa acatgaatac taggaataaa acggatgaat agaaaatcaa
    12901 aagcagcaaa aaaaacgacg acacctaaaa taaaattata agagaacttg aatatattga
    12961 ttctctcaat ttgattttcc agtggatagt caggtagtgc catgtgtttt actttgaatg
    13021 agatttgcgc ttgaagggat ttgaacccct aaccttctga tccgtagtca gatgctctat
    13081 ccagttgagc tacaagcgcg attttactaa aacaggtttg tgacaatata ctcgtagtgt
    13141 ttgtcaatga ctgccatatc aagagtttta tttctacgat aggagtaaat aaaaaaacca
    13201 gaaagtccta agtatgctag aattccgaca agatgggcta tgtttgcaaa aactgaccct
    13261 ataaatggaa ttaggtttaa agccatacta gctaggaaac aggcaaagaa atagaaggaa
    13321 aataacatgg agtaaatagc actctttcga atgactaggt tttctgattt aaaaataaga
    13381 aagggcaacc agttaaaaaa aagaggtaac atcgcaatat ctcgcatccc ttcttcggaa
    13441 cgaacgtaat ttaaacctaa atctatgtag gttttgattc gttttagata tttattttga
    13501 tgaatagtag ctgattccaa agaagtgttc caagtatttc ggagacgccg ggattcgaac
    13561 ccggggtaca gttacccgta cgacagttta gcaaactgct cctttcggcc tctcaggcac
    13621 gtctcctgtt cagaatttgg cggagagggt aggattcgaa cccacggtgc tcgtcgcacg
    13681 acggttttca agaccgcttc attagaccac tctgacacct ctcccaaaat tccaatttct
    13741 ttgtctttcg aagcgccttt cctgtcaagg gaattcatgt aaaaggttgg attttatttg
    13801 taaaaattct tactaactca ccttgtccaa ggtgactcaa gcgttcaaat gagatttgca
    13861 cctattgtca ttgaaaaatg acaatgggct tgagcaaagc gaaagagatg gaacggaggg
    13921 cttgcggccg caggcttttg attttgttag ttcattagca tttcataata ttattaaaat
    13981 catttctttt tactttaagt gtcaatttta tgtaaggtga gttactaaat cttttcccaa
    14041 tcgaatgcag tgatattacg gtctgtttta ttaaactcaa cgccgatgag ataaatttca
    14101 cttacagtgc ctgatcctag atgagtgctt acgtatccga tgtatttctc gtggtatttt
    14161 ctttctttca gttgtgcgat tgctttaccg ttaggtgtca tttcgtttac tttaaattct
    14221 aaaatataac aacgaccatt gagtatagca accatatcaa tacggccttt gttgcttgca
    14281 tcttctactt ttacttccac tccaatggct gtgaagtatg tatagaaaat ggaagaataa
    14341 taaccttcat agcttgcgat ttcattattt gtataccaat tgtagggaat cgaagcaaag
    14401 aaagcgtgaa ataaatctcg cattccattt atatcgccgg cttctaataa acgatataat
    14461 ttcaccttat tcagttcttt ttctgtgttg agagttgata agtatttcaa aataacatca
    14521 tttaaactca ttctaacttc tttgtttggg tataccattt catataacgt catatctccg
    14581 actttttgta tagactctat cgttagatat cctgtctgaa ataaaagtgt ttccagttca
    14641 atggagccaa tgtcaaagct attcagagaa ctttcggtga gttgtatatt ttctaaatct
    14701 ggaattggaa attttctttt atcaattagt ttaattaaaa agctaggagt tccagtctcg
    14761 aaccaatagt ttttaaagtc ttttgttcta aaataattta aaacatcata gggattgtaa
    14821 actttatctc cacagaagtt atatccatta taccattctt ttaattgatc cattggaacg
    14881 tcagtcaata gatcggcaaa tgtttgattt aattcttttt gtgtatatcc acaaatacta
    14941 gcaaaatcag gatcgagtgt aatatcggtt agattattca atccactaaa taaattgatt
    15001 ctagaaaatt tactaacgcc agtaataaaa acaaattgga tataattatc tgagtcttta
    15061 ataacagaat aaaatccttt tagaatttct cttacttcat agggaacgtc aggcttttcg
    15121 atattgtcta agattggttt atcatattca tcaattaaaa ttacaacttt ttgtttatac
    15181 ttttcaaaac tagattgaat taattctgta aaacaattcg agattgtttt gttttggcaa
    15241 gaaattccta gatgcttttc attcatctgt aggatgcctt ctattttctc taaaacatct
    15301 ttactgcttt gcatgatccc accaccaaag ctaattttaa tcacaggatg ttttttctgc
    15361 cagttccaat tcttttctag aaagagtcct tggaaaagtt tttcatttcc ctcgtaagct
    15421 tctttcaaag tatctaaaaa agaacttttc ccaaatcgac gtggacgaga gagaaagaaa
    15481 tatttaccta cgcttgccag cttatggaca aacattgttt tatctacata gtaatgattg
    15541 tcctcaatca tttctgaaaa tgtttgaatt ccaagtggca gtcgcttcat agaattaaac
    15601 tctatgtgaa atgcaagctt gtcaagtttt taaagtgtag aaccagctat gctataactc
    15661 aactacggtt ttcgattgaa aaataattta gaaagaaaga atctagatgc atgagtgtag
    15721 aagtaatttt gttagcgatt ggttgctatt tattttgtgg gattaatgat aaaatacatc
    15781 ttatgaaaac aggcgattgg agagaaggag ggaattatac taatttagaa gaaactattc
    15841 aaaagctaac ccttgctcca aatgaaacta gctatgaatg gataattgaa aaaataggaa
    15901 agaactttgc tcgtcaaaga acacacagag tgattagaaa gcaaaaatat aatggcatag
    15961 tatattattt taataaggag ttacgttata ttaaccgaac tggtcataat tacgaaaata
    16021 gtgtagccta tgtatcatgc actaacgatc tagaactcaa tcttttcttt agagataatt
    16081 tattagtatt ttataatata catgattccc gaatggatag aaattgtaat gagtcatttg
    16141 gacctttaca tacaatacaa gacaaaagtt ggttaggagg tggcgaaaac gatgcatcat
    16201 ctccaaatag ctgtaatgaa aagttttata ggatatttac attagataaa aaataggaat
    16261 gggacaatgc attggattgt gattttaaaa aggattttaa aaactactgg gctgatcccg
    16321 tttatagcga taggcaatat gttacaaagg atactggatg gccatgtggt ggtattttta
    16381 attcatgcta agacctacaa aatccatctt ctactagcga aagcgatcaa ccgaatgccg
    16441 tgcatgaggg cgagcgcggt cggtgtgttg tgaaaaacgt agagacgcat ggcgtgcgtc
    16501 tatttaccga gaggataaca ccgattcgcc ccaatcgact tggatccaaa aagattttca
    16561 ccacgaagag cacgaaggtc acgaagaaat ggaaggaact agattcttca tccgtttgat
    16621 tcaatcagaa tctttctgac cagaataaga ttgcattcac gatttacaac gtatattaaa
    16681 aaaacgtaag aaatgcaaac catctgaata aatatctctc tcccatcggt gttcatctgt
    16741 gctcatcggt ggtaaccttt gaattcgtat ttgcaaagaa ttggtttaca ctcactccta
    16801 tgcaagcaag tctgacaatg taatatgatt ttaagagtag aaaatctttc taaaacctat
    16861 cagcctttct ttttatctaa aaaggctcct ttacacgctg taaaaaacgt aagcttcgag
    16921 attggcaagg gagaaatcta tgcccttctc ggtcccaatg gagcgggaaa atccaccatc
    16981 attaagatga ttgcagggct tatcaatccc acagcaggta aaattatttt taacaacgag
    17041 ccagtcacta aaaacactgt ctacaaaagt ctgagcgcag ttctagaagg aacgcgaaat
    17101 gtttactggc gactgaatcc acttgagaat ctacactact ttgcaaatct acgtggaatt
    17161 tcttcttctg aagtaaaaga aaaagcaacc gctcttttag aaactctcga gatagaggga
    17221 aagaaaaaaa atcaaagcca acatctctct cgtggtatgc ttcaaaaact ggcattaggt
    17281 gttgcgctca tcactgatcc acaaatccta ctgttagatg aacctacttt gggtctagat
    17341 gtatctagtt ctcgcaagat caaagaaaag atttttcaac tttctaagaa agaaggtaaa
    17401 tccgtattac tcacaacgca tcaaatggat ttagtggaag agcttgcgga tcgtgttgga
    17461 attattcgaa gtggttctct tgtcaaagaa ggaagcattt ctgatctcaa atctattttt
    17521 aagagttaca tctatcgcat taaaatcaaa ggggatttta aacttcctcc tgtttgggtt
    17581 aaatcctata gtgctgaaat caaaatggag caaagagaat atacagaaat cgaagtagac
    17641 tgtaaaaaca aacaagggat gtataaagtt ttacatgaac tggaaagtct caagaaagat
    17701 attatttcct ttcatcgtat agaagaagat ttagaagaag tttttttaag ggtgctatca
    17761 aatcaatgat tcgagtaatt acatttattt acgatttttt attaatacta aaagcagaag
    17821 taaaaagaga ttatatattt ttaaagcgtt atccgcttga gattgtatct tttatattct
    17881 ttatgtatat cattttactt gctgtatttt ttggattctc tcagcttacg attggaagtg
    17941 tgaatttact caatcgtgag aaaatgatta ttggttactg tctaatgcaa tttgtattgt
    18001 ctactcaaat gggttggtct ggacaaattc aaaatgaaag tcagaccgga accttagaac
    18061 aactttctat atcaggacat ggtttaggag aagtcttact tgctcgtgga ctcgctcaac
    18121 ttccaagaca agtaattagt ttttatactt tgctttttat gtataaaatg agtatccctg
    18181 atttacaaat tagttttacg aataattttt tcatcttagt cacacttttt atcatggcga
    18241 ttggtatttt tggggtctct tatatttttg cagggattac acttcttttc aaaagagttg
    18301 gattcttttt tcaaatcatc aactttgcat tccttggact tttttggcaa gatagaagca
    18361 agcttgtgaa tgggagtctc attgctcatt tgtatgataa ttttccactc acaatgggaa
    18421 tggcaaatct tcaaaagatt ttaatcaatc aatatgtaga attaaatatt tctgaactca
    18481 atttgttaat ttttatgctc aactctttgt tatttgcggc atttggatac tatctatttc
    18541 atttgatgga acaaaaggca agaacactcg gtcttctttc gcagtattga ttttgggaaa
    18601 taaggcaaat tcatattagc cgcttaggct ttgaaaggat aaaaggctgg tcataaatac
    18661 atgggctgtt tcgattctca gaatattttc ttggattgaa aaagaccttg catttgcttg
    18721 aatgagtaaa tccaattctt cttttctaaa tccaccttcc ggaccaatga ttggtattga
    18781 tgaccagtct gaatttgttt ttagattttt cttggctttt ggatcgagca aaaaaatatt
    18841 tttttcagcc agcagaaatt tttctagaga agaatagact tcgatagttg ggatcgtatg
    18901 acgtttcgat tgggagcatg cttccagaat gattttattt gctcggtcta ggttgatttc
    18961 ctttctttct gaatgtagaa agttgataaa ataaaaatgc gtaattccta tttctgtgct
    19021 tttttgtaaa agaaaatcga gtcgattcga ttttggaata gcagaggcta ctccaatttt
    19081 attgctatta attaagtgag tctctgttcg aattaaatta ccggttttac tttttgctcc
    19141 aacttggaaa aaataagagc tgccctttcc atctcttatt tcaatttctt tttcttcatc
    19201 attgattctc agacttcgaa agtgttcaat ttcttctttt gtaaatgtga tttgatttgt
    19261 gaacttctca tctgtgcgaa atagaatcat atgaataata tgacccccat agaatcgaga
    19321 ttgtgtctac catttaaaat ttttagtaac tactgttaaa taaatttaat aaattgaatg
    19381 ggaaatgatg aatttaaaaa tattagagaa tacaaatcaa cgaataaaat caatagcctc
    19441 tgatagatcg gcttctggag agcttataaa gcgatagata gacaagctct tccttccttc
    19501 tatctcactg tgcgcaagtc tcttgtaagt tttcaatgaa aataggagca aacctctttt
    19561 gaacgtttgg ttcacctaaa ctcaatcatc cttttgttaa gcaatgataa taacgatagt
    19621 gtaagatgag ttataaaact tcttgctctt ctctctagca aataaattct ttccctatga
    19681 agaagaaaga tagttcctct atttcccata tgcgtcgtaa ttatgcactc ggtggattaa
    19741 tagaaacaaa catagaaaaa agtccaatcg atcaattttc gatttggttt aaagaagcgc
    19801 tcaattccaa aattttagag gcaaatgcaa tgactcttgc gactgccgac aaatctggat
    19861 tagcctctgc aagaattgta ttattaaaga gctttgatga gaaaggtttt atttttttta
    19921 caaactatga aagcagaaaa ggaaaagaaa ttcaagaaac gaaaaaagga actctccttt
    19981 ttttctggga tgctcttgag agacaagtga gaattgaagg gaagttgaag aagatttcta
    20041 cgaaagactc cgatgaatat tttcattcta gaccatttga aagtcaagta ggagctatcg
    20101 catcgagtca gagtaaagtt ttgaaatcaa gagaagagct agaaaaaaaa tattcagcac
    20161 tactcgtcca atacaaagat aaaaaagttc ctatgccaaa gcaatgggga ggctatatat
    20221 tagaaccttc taaaatagaa ttctggcaag gaagaccaag tagactccat gatcggattc
    20281 tgtattctaa aaataagaaa ggttggaaaa tagaaagact ctatccataa tcttatgcct
    20341 acgaaatctt atacctatta tgattataca atcagccttt gcacaaaatg tttaaaacga
    20401 atcagtgcaa agattatctt cgaggaagaa aaagtattta tgctaaaatc ctgtccagag
    20461 catgggacag agagggtttt aatcgctaca gatattgaat attacaagcg atgtagagat
    20521 tttttaaagc caggagaatc tcctctccat tttaatacag aaacgttata cggctgtcct
    20581 tatgattgtg gtctctgtcc ggatcatgaa caacattctt gtctaactgt tttagaaata
    20641 accgatagat gtaatcttac ttgtcctacc tgttatgctg agtctagtcc tagccatgga
    20701 cgtcataaaa ctctcgaaga aattaatacc atgcttgata tcattgtggc aaatgaaggt
    20761 tatgcggatg ttgtgcagat cagcggagga gagccaacaa ttcatccaca gatatttgag
    20821 attatagctc tcgcaaaaac aaagaagatt cgacatttga tgttaaatac aaatgggatt
    20881 aaaattgcaa aggataaaga gtttgtaaag aaattgtctg aatttaaagt agggtttgaa
    20941 gtctatttgc aatttgattc ttttaagaag gaatcattag agacgctccg tggagaggat
    21001 ttaagagaag tgaggcaaaa tgccattacc cacttaaatg aatttaatat ttctacaaac
    21061 cttgttgtca cagtgcaaaa gggtgtgaat gatgatgaaa ttggttcgat tatcgattac
    21121 gcacttactc aaaaatgcat tcgaggtgta accttccaac caatcgaaat cgcgggaaga
    21181 ttaacccaat ttaatccagc aacagatcgt tatacgatga ctgaaattag aagtgcaatt
    21241 ctagaacaaa cagatgtatt tacagagaag gatttaattc ccgttccttg taatccggac
    21301 gctttggtga tgggatatgc tcttaagata gataaccaag tctatccact gactagtatg
    21361 ctagatccag aagaaatttt acaaaatact ggaaatacaa tcgtatttga aagagacgaa
    21421 aaattaaaaa gtcatctctt aaaagttttt agcactgcaa actctgttga ttcaattact
    21481 ccagaacttt ctacaatgct ttgctgtctt ccgaatatat ctgctcctag cctaacgtat
    21541 gagaatgtat ttcgaattat catcatgaat tttatagatg catataattt tgacgtgaga
    21601 tcagtaaagc gttcttgtgt gcatatcatc aatgatacaa gtggtaaaat cattcccttt
    21661 gaaacgatga atctttttta tcgtggagaa aaaattaaac aactagaagt attaagaaag
    21721 agaggttagt atggatgaaa gtgttttaaa gaaaatagta attttaaact taaaaatcta
    21781 tggtcttatc accttgtgcg agttaatttt agttgctggt tttattgggc agggagatca
    21841 aaaatacggt ggagtatttt ttatgatact ttctatgttc agtatttttt tacaggtttt
    21901 gaccaatata atcctctcta tttacaatta taagaaagaa gataaagaaa aggcaaaggc
    21961 ttatttatta agtttcattg tgatactttt agttgggttt ccgagttgtt ttggatttgg
    22021 aactgtaatg agtattcgtt aatttatata ctatactgat gctacttgga attttgattg
    22081 tccttgtgag atgttagccc tgagcgcatt aaagggtcga agcatagcta atagaatcag
    22141 tattcatttg acatgctcag gattacacta tcgctatttt ccgaaattca atttttcttg
    22201 tgtatatcga ctagcagtct ctcgaaatta ttaataaaga atctatcgca aaagatttaa
    22261 attcgatcca gttcaattct gctcaatcat ttctagaatt tcttttctta ccaattcata
    22321 ttcattccat ggtaggaaat gattcatatc tcctcgaatg atttttagtt ttgttttgtc
    22381 agaaatcatc ctttgcgcat aatccacatt agccggtaaa accaaactat cattgtcgcc
    22441 ttggatgaga gtcattggaa tttttatatt tttccaaaga tgttccattt ttttgagttc
    22501 aaatcgaagt ggtagaattt cttgattgga tacatctaaa aaacttggta aaatgaaacg
    22561 aaccaatctc caattggcta atttttgata ccaaagaact ttttctaatt caggatcaat
    22621 cgaacctgct aaaagtaata cactcgaaat atattttggg taatccattg caagtctcac
    22681 tgctacaggt cctccataag agtgaccaat tactaaggta ggaagtctat atttttctag
    22741 aataggagcg attgactttg cttgatttgc aagtgataac tctgctttcc ccttgttcga
    22801 accgccatat ccatatcgat caacagaaat taaaactgct tttttctgta agatcttatc
    22861 ttccaaataa tcagagaaag cttttaagga gccaggggaa ccgtggataa ataagattag
    22921 cttctcttga ttagaaggag ctaccgcata ttgcatagta gatccttcta tattgtattc
    22981 ttttaaaatt ggttttattc ctttctcttc ttcttttcgt tcagtaaaac aatagaaaca
    23041 aaaaagtaga agaaaaatta agattcgcat attacttttc caaatttcta acgagcctat
    23101 ccatattttc ttgcatttct tttctgaaaa tcccagaaag aataccttca cttaaacgca
    23161 aaagaccttt tagttcaatg ttgtcagtga ttctaagaaa tgtattttct cctctatctt
    23221 cgaactcata gtagtcatca aattttaaag tagttgaatc acagcttgct aaaaagcttt
    23281 ttcccttttc ttctgagata atacgatagt cggaatttat atttactata gataaactta
    23341 gagtgattgc acatgagttc ttctctgagt cagtccattt tgcatgtttg agcgatgaat
    23401 tatattcaac cattgtttct aaatttcgca cataggaata cactttctca ataggtgctt
    23461 taattactgc ttctacttta gttgtaaaca tagattacca tttaaagcta ccgcttttgt
    23521 cataatcgaa actcatcgtt ccatcaggta aaccagggac attgagccca gaaagaccct
    23581 ttaatttaaa atcagagctt tttttctgaa ttgcatctgc aatcccactg gaaagagatt
    23641 tgagtggaaa acttgttttt acttttacga tggattcttt tccattgtta attatatcga
    23701 ctggaactcc tgataggaat ttttctccgt taagcgctaa gtcatatttt aaatcactga
    23761 actttagagc tgctgccgcc tgatttgcca gtgcaatttc aaattcggtt gcgacatcaa
    23821 tatctacatt gctaagacct gcttgcgctg ctgaacctag atttgttttt tttccactga
    23881 gtaaattatc taaataagtt tttgctgtgt ttgttgctcc atcttttact ccactactga
    23941 tttgatctgt atctggctta acgattttaa aattacgaat gtcaatcgtt ggaagaacgg
    24001 ctggaatttg cttgtcctgt gtaaaaggaa attcaaagct tttttttcca gctaattgat
    24061 aactctcagg aatagggaca tttaacactc catcaagttt gacatttaat aaatcttttc
    24121 caggaagttt tttgtaaata tccataagat cagaatattt cagtataacg tcaaccggaa
    24181 gatccttgtt ggaactagct tcgatttttc ctaaatcaac cttagagaac tttgcgagat
    24241 tcgttccttc tattaataca ttcatgtcta agattgattt tggaatcgat actgggtatg
    24301 gatttctaac atttgttaaa agttttaatg atatattctc aagtgtgatt ttatttatgt
    24361 ctacactttt taatgtgaac gttggctcat tcccaccacc taaaaattgt tgaaacagtg
    24421 cacaaccatt gaaaacaaaa atggctccaa taaaaaagga taatttgaat agatttttca
    24481 tgatctagtc ctttatgtaa ttattttact gagtattaaa agcgaatcta tttaagaata
    24541 aaatactaca agaatttaat ttgattcgga tttaaaaaat ataatttaac tttttaaata
    24601 aaatggaaaa ttatatttca tctctaaata gaattatttt gctttaaaag tcagcataac
    24661 taaaattatg tattgggata gaagggtcaa gataaatgat tttgattttt tagttgataa
    24721 agaattttct ctaacatttc atctacttga attggctttg agattatatc attcattcct
    24781 gcttctttta cttcctcgaa aatttctgct tccgaacttg cagtgattgc aagtattgga
    24841 acattccgta tatcctcgtt ctctgaggct cgaatccgac gagttgtctc aaatccatct
    24901 agctcaggca tttgtaaatc catgagcaat aaatgataat gatttgtttt aattttttct
    24961 aatgcttcaa gaccgttcga ggcgaagtca aggtctacat cccatttccc tagaaattca
    25021 gaaacaacaa tttgattgat agggtaatca tcaactagaa gaattcgaat cccattaaag
    25081 aagtcagaat aatcatcttt tttggtatcg gggattttct taattatcgt gttttcagaa
    25141 tttttaaatt ggatggcaaa attaattttt gttcctttcc caatttctgt ttcgataaaa
    25201 acagcactat ttaggaattt gagtagctta taagatatcg ctaaaccaag ccaagattta
    25261 tataatccat catttacgat tggaaaattt gggtttacct tttcaaaaat tgactcaacg
    25321 ctttcctcag ataagccaac gcctgtatca gatatggaga atccaattgt cgaataactc
    25381 tgatttacct cttctagttt gacctcaaag catattttcc cgttatttgt cctatgaatt
    25441 gcattgacta taaaattaaa agcgatttga gagattcgta ttggatcgcc aattaaaata
    25501 gaaggtatat tttcatcaaa aatacttata aattcgagtc ctttttctga tgcatttgcc
    25561 tctgcaagat ttttaattgt cgttagtaga tagactagat cgaattcatt ttcatcaaat
    25621 tcaaaattac cagaatctaa taaattaaag tctagaatgt cattgataat gatcattaaa
    25681 ttctcaatgg cgagtcgtaa attatttaca ttctcaatct gatctttttt tggatttttt
    25741 ctttctaatc tatcgacagg ttcaagtaat gtgttcgcag ccaatcggat ctcttcactg
    25801 atggaagaaa gaaaaaatga cttacttaga tttgctgctt ccgctctttc ttttgcaaga
    25861 gcaatgtttt tttgcatctc cttgcgttta gctatatcga tactggaaaa tattacgcct
    25921 tctattattt tcccttcttt atacataggt gaaaaactga tttcatacca tgattgatgt
    25981 ttatttgatt taaaaaaacg ttcaaatttt aaacttgttc cctttaaagc tttttcaaaa
    26041 ttctttatta caagtttttg atctctcggt aaaaaaagat ctaaaaagga atcatctaat
    26101 tttaaattcc ttccaaataa taaattggct gactcgtttg cgagtttgtt aaaacttcta
    26161 atctttaaat cacgatcgaa gaggataata ttgagtaatc catcattata aatggcatta
    26221 agatttgctt tttctgctga gagccttttc tctacttcat gattcgcatc tacaatatta
    26281 tacaaaatat atccagtcaa aacaagtgct ccaaatacag attcaagaaa taaaactcgt
    26341 tctccttgaa taaaatcaga atcataaaag ctaattggga aattataata aatacttatc
    26401 gataaaagta ggattggtaa acaggaataa aatatcagtg gcaactcttc attaattcca
    26461 attagaaaaa tacttacgat gataagcgga aagaaaaaaa gaaaaattcc tgaatgaact
    26521 ccttttaaaa tagataaacc aaaaataatt aaattaaaaa gaatcaaaat aatatgaata
    26581 aaaattttat gcagttcaat ccgatttaga ataaataaaa ttgaaaaact gatggaaata
    26641 caaaaatcca aaaaggcaaa tagattgtat ccattccaaa aatctacgaa ggagtttata
    26701 aagtgaatta atgcaatcaa aaatagaaat cgattgatgt tggacattgt tcggattgtc
    26761 tctatcgagt attctcctgt tatcccatac attgtgattc gattccaaat tatcttaaac
    26821 atttgaattt ttaccagcca ccaagaattt ctattccaga atgattcatt tttataaact
    26881 ctatagagaa gtaaatattg gatccttttc caagctcact ttcaatatat atatggctgt
    26941 caaataaggc taataaccgt ttaatgacag tgaagcctag acgaaaagaa gggcgatttt
    27001 ttatgtattg tgtctctggt tctctgaagt tttgaactaa ttggcgtaat gtttttttag
    27061 atatccctcg acctgtatct gaaatggaaa atcgaatcgt aacttttgtt tctgaatttt
    27121 ttaatatcga agcattgaaa attatttttc ctttctctgt aaaatgaaga gagtttctca
    27181 ataggtgaga aagaatctgt cgaatccatg caggctctgc cataacgtat tttggaatgt
    27241 cttcatcgac gataaaaata aaatcaattc ctttttcttg cactcgatct ataaataaat
    27301 ctcgaataga aagtaattcc gattttaaat caagtttgga attcccgatt tgtaaatttc
    27361 ctgattctat agctgtataa tctaatattc catcgattaa cctgtttaga gtaagtgatg
    27421 atttgtttaa tttatcaaga atgagtattt gatctgttct cggatttctt tctgagaggt
    27481 tttgtaagct tatattgata tcattcataa cttttcgaat ttggttgctg attgttgata
    27541 aaaaatggga tttactatga ttgactgcta ttgctttctc tcgtgctatt tgagattcta
    27601 tttccaattc ctttcgatcg gttatatcta gtaatgtaat gactattcta ttatttatat
    27661 tttccgtttt tatagaacta acggttagat caaaccaatg ggatgtatat tctgttttaa
    27721 aattcttttc tatttgaatg atattccctt gttctactaa attgagataa tgatataaat
    27781 tacctttttc ctgatcaggt atatattctg taagttctct acctattttg atttcataga
    27841 ttccaaactt tataaaaatg tttttaaagt ttatgttgta agaaattatt tttctagatt
    27901 cgtctagaat tacaataccg agggggctat gatttaatat taacttgagt gtatcttctt
    27961 cctcattgaa tgctatttcc attttgtttc taattttgat aatactatag atacaaaaaa
    28021 acataaaaat agcagtcgat aaaagactta tttgaaaaat tttactttgg tcaaagatgg
    28081 ttaactttga atccaataaa agagaataat tagttatatc gaggataatc ataagtaacc
    28141 ccgttataaa aaaattatac aaaatgaatc gtttgtgttc gtaactaaat ataaaaaagt
    28201 ttaatagcaa tacatcaata aaatatatat aattaccagc ttctttgcct gtaattgaac
    28261 ataataggaa agttcctatg ttggaataaa gaactagata aaaactagcc gctaaatgaa
    28321 aaaaatgata gtttaataaa aatactggaa gaatcaaaag aaagaaaaga aaatatataa
    28381 aggcatagaa ataaaaattt aaaaatgaaa aaacaatgat atttaaaaga acaatgagcg
    28441 cagctatcaa tgcaatctta ttgcagtaaa taatagattg attgttaaga tcagggttgg
    28501 tttttacaag tcctaactcg cttactgtat tccagatctt tctaaaggtc ttcatctttt
    28561 tgtcaaataa acacaatact tattggtttt ctagtaaata atcttaaatg gaaaatattc
    28621 tcaaattttt atttattcta gattcagatt tcattatatc taaaaataaa tgaattagag
    28681 tttttgtaag ttataattct agtttacagt taaggaatga ctattcacct tttctaaaat
    28741 ggactcaatc aataatttta aaattaaaat tcaaatccaa tactataaaa tcctctcttt
    28801 gcgttattgg gagttctggc cagcttggtt tttttatata ccaatatttt tttactacct
    28861 agtatttatg ataaaatata gaatgagcgc aactgcgatt gcgggtgtaa atccctgttt
    28921 tgcatttggt gggatggcat ttgattctaa gtatgatatg atttctaagt ttctcgcttt
    28981 tcctgatgca atcgcaaaaa ctgttttaat ttctaaaaga gaaattgtaa gtataatcaa
    29041 acaagtcaaa gaggcaaata tagattatcc gtttatatgt aaaccagata aaggacatag
    29101 gggaaatgga tttaaaatca ttcgaagtca agccgactta gaaaattata taaatatttg
    29161 tgcaagagat tttctgattc aagagtatat cgattatcca ttagaatatg gagttttttg
    29221 ggtgaagtat cccggggagg aaaaaggtag gataacatct attacccgta aagttcttcc
    29281 ctatttgaaa ggcgatggta aatcaaagtt atatgaattg attttatctg ataaaagagc
    29341 aaggtattta gcgaagactt acttggaaaa gaatcaatct aaaatagatt ctgtcattcc
    29401 aaagaatgaa tctattagaa tatgcagtgc tgggaatcat tgccaaggag caatttttga
    29461 agatggaatg ggtgatatca aaggtaatac aaaagagagg ctaattgaaa tatgctcctc
    29521 tatttctggc tttgattttg gtagaatgga tattaaattt aaaaataaag attctctcga
    29581 aaatggatct ggattcaaaa ttttagaagt gaatggaagt gaagcagagt ctactcatat
    29641 ctacgatcgt aaatatagta tttattatgc ttataaaaca ctctaccaac aatgggatac
    29701 attatttgca atagcacgta aaaataagga gaaaggattt tctagattaa cgataggagc
    29761 tttattaaag gcatacattg aattttttaa ggaaagtaag gaaataaaag gggattaaaa
    29821 aacaggagaa agacacgaag cgtttaaccg ctccgtgtta tttaattcct tacttagctg
    29881 ctggtctgct aaatgttgct gtaatactag tttttgcaag ggtagcaaaa ttaatattat
    29941 agccaacgag tgctccggaa gcttctccat ttacatctaa cttatctgtt ttgagtgcat
    30001 aaactgtgat gatatatcta tgtggtttat ctcctggtgg aggacaagca ccaccaaagc
    30061 ctggcttacc aaaatcagtt cttccttcaa ctgcaccttt tggaagtgct ttagagcttg
    30121 ctccttcttt taattcagtg acgttagctg gtatatttac taatgtccag tgccaccatc
    30181 cgcttcctgt tggagcatct ggatcataga cagtaacggc aaagcttttt gttcctgctg
    30241 gagcattttc ccatttgagt gctggcgaaa cgttttctcc agtgcagccg aatgagttaa
    30301 aaacatgttt attaattaga gttccaccgt ctttaattgt agaactagtt aatttaaaat
    30361 cagcagcaaa catgctcata gatgtaagcg ctaaaataag tataaatttt ttcatacata
    30421 acctctctaa aatccaatcc tgtaaagatt gggttttaaa tcaataattt acgaaagaga
    30481 cgatctggtt taaatttgtt tgcttttaga aaagcataag gaactagagc agtcatagtg
    30541 gtttaagaga tagaatcgag agagaagagt atgattgaga gattatttta agagagaagc
    30601 gtcgtttaac ggctacaaag tatagaaata ttaatataaa atgagagaat tcttttggtt
    30661 gacgaaaaca gaaaagaatt caaataaaag atatgaacat ccttctcctt tttcttttgt
    30721 ctgttttacc tggtcttttt ttaatctaca gatattacaa aaaagatatt tataaaaaag
    30781 agccatggac attgatttgg atttcttttt tcataggcgc agtcactgtg attcccgctg
    30841 gtttattcga atcatggatt gaggttccaa acaaagaatc gatggaagga atgattcttg
    30901 aaaacttttt agtaattgct cttactgagg aaattttaaa gttattagct ctctattttt
    30961 ttgcttataa aaataagaat tttgatgaaa cgatggacgg tatagtttat ggcgttgcgg
    31021 tgagtagtgg atttgctact tttgaaaaca tattttatgt aatgcaaaat ggttttactg
    31081 ttggaatcat tcgagctatt ttatctgttc cagctcatat tttttgggga gcaattttag
    31141 gatattggct tgcaaaagca aaatttcatt tcgtttcttt attcaaagca gtcctgatcg
    31201 gattgtcgat agtcgttata ggtcatggct ttttcgattt tgttttaacc tataataatg
    31261 cggaatactt tgcttttagt ttacttcctg tcattgcact tgggttatta gtaaaaagat
    31321 atgttcgctt agcattggag tttgatctaa aaaatatcca tacacatatt gaattagagc
    31381 aggtagaaaa tcaaacctca gtattagaaa aaaaatcttc tgaagctttt atgatttctc
    31441 tacagagctt aatctctatt tgtctttatt ttttgggtat tatctttctt ctaacaggtc
    31501 tttttttact gattggtttt atagctctac tccaagaaaa caaagctgaa acttggacta
    31561 tctctttcgc attgattcca atactagcag ctatttttat gatttataaa gcaaaaaaaa
    31621 ggaaagggaa taagagaggc atttaactgc ctcttttgaa atttatttga gggcttgttc
    31681 gattgaatcg tggattaaaa cgaaatctgt caacccaacg atatccataa ctttgcggaa
    31741 atgagagttt agaccagaaa attcaatttt accttgattt tcagatgctt ttgtgattaa
    31801 actgattaaa gttgcaattc ctgctgaatt gatataggct gttttagaaa aatcaattaa
    31861 aactctagtt tttttggcag caggtatgga tgcatagcag ttaacaatct cttcatctgc
    31921 ttctgaggta atttcccctg caatgtgaat aattggtatg accccattgt catagtctac
    31981 atgtatttta aattcatcta ccatagggat tataataaat acattaaaat tatcaagtca
    32041 agacatttta ttatttaatt ttctttaaaa ttgcggaaga gaagtgcttt ttacacttct
    32101 cttggttgta tcagattagt tttttgtgag gtatactaca tatttagtga tagattttaa
    32161 tgtatcatca cctaaatgct tgtaagcaac cattgggctt cctttaattc cttcatttaa
    32221 ggttttaaga attcctgcct cattgtttcc atttttccat tcgctagcag gtgctttgta
    32281 atttcttggt tttggattga gagatgctgc cgcaactccg tcccctgcac cagttgcgcc
    32341 atggcaagaa gcacaattga tatcaaaagc ttctttccct tttgctactt caggatctaa
    32401 ggtagctgtt ggggttgctt ctggggttgt tttagcctct tctttttctt ctttttttcc
    32461 gcaattaata gcaactgcaa aaacaagaga taataggatt aacttaataa tgtttttctt
    32521 cattcaaaaa ctcctttgtt ataattttag tttttaattc attacttcta tgaaaaggaa
    32581 aattttatag atcaatttac tttaatagat tttctaaagc tgtttctaaa ttagaaaact
    32641 gaaattgaaa cttaagtgca tttaattttt cagggagaac attttgtcct tttaaaatca
    32701 cctctgctcc ttccccatag agcatagtaa ctgcaagggg tggaacattc aataatgatg
    32761 gacgacttat aacttttccc aatgtctttg agaactcata attagaacaa ggatttgggg
    32821 ctgttagatt gaatatcccc gtttgttggt tctcaataat aaaacggatt gcacttacta
    32881 aatcatttat atgaatccaa ctcataaatt gttttccact tccaagagga cctcctacaa
    32941 acattttaaa aggtggaagc atttgtgcga gtgcgcctcc ttcttttgct aatacgattc
    33001 caattcgaat tgttgcagtc ggtattccaa tttcatttgc acgcgatcct tctttctccc
    33061 agtccaagca tagctttgca agataatcac ttcctgcttt ctctgtttcg ttaaacactg
    33121 gtttgccgct atcatacatt ccgtaataac caattgctga accttgtatg aagagtttag
    33181 gttttgtttt taattcagat aaatcatcaa ttagctgttt agttttgtcg actctagaac
    33241 tacgaattct ttttttagtt tcctctgtcc atcttcctgc taaaatagat tctccagcca
    33301 agttgataat tacatcaatc ccttctaaat cagctgcttt tgggtgatct gatgagatta
    33361 attttacgtg agatagattt gctaaatctc ttggcaaaga attttttcta gatactatgt
    33421 aaacaatatg gtctgagtat gacttagcca aatgtttacc gattaaacct gaaccgccta
    33481 tgattgcaat tttcattgat ttcaaaactc cctatgaatt aaatgacaac atcaattatg
    33541 attaagtata tttctatttt aatttttata tctttgattt atttgaatta ttctatttat
    33601 actacgacgg aaaagaaaat ggatatctat tccaaaaatc ctccttttcg tctagatgat
    33661 tttacaaaat cttatcgcaa aaacaaactt tcccaagtaa aaaatttacg agatcaggat
    33721 tcttcaactt actggataaa agagcagaat tcctttcatc cagagtatga tttagaaaca
    33781 gaactaacgc tcactcattt ttataaggaa ggaatattta aaaagaaaaa tttaaattca
    33841 attacaattc attcctgtaa tccaattgca gataaatcct ttttagttaa gccaaataat
    33901 ctagtgatag atatttatct gagagaagca atcaatgtcg acaaagagct aagacttccg
    33961 aaagatgaga agatctccag tatattactt tcattccagg acaagacttc tgaaaaagta
    34021 gatcttacta atattttaaa attggcagac tcaaaagatt atccaatcgg tatatatatt
    34081 ttaacacttt ttatcaagga taattctaat tatccgcttg ataaaaaagt ttgtatttct
    34141 gagatcacag tcgaggaatg agtcaaagtt ctacaataat tctccgatca gagaggtggg
    34201 ttaataatgg ttattctatc ggatataaag atggagaagc ctattttatt ttaggggcaa
    34261 taccagaaga gttagttgaa tgtaaattga tacactcttc aagtaaaagt agggaggttc
    34321 tagttgtaaa tgttcttgag ccttctaagg atagaattca atcagactgt gaaatatttt
    34381 tatcttgtgg tggctgtagt tttcgacata tagaatataa actagaaata gacttaaaaa
    34441 gaaatttatt gcaaagagaa ttgattcata agatacctga atggaaagac tctttagaaa
    34501 gtataaatgt cgttagtgga gatagcacag gatatagaaa caatactcaa attaaaattc
    34561 aaaatgaaaa gaaaggcttt ttccgtttgg gatccaatga gttagttgag tttccaaaaa
    34621 ctggatgcaa aaatctaccc gatgaaatta attcttatat acaaaaaaat aactttaaca
    34681 acaaaaaaga aataaaactg agacttacag acaaagtttt tcagtatgaa acagttgaat
    34741 caatttttac tcttgatagt ttaaaaataa aagtagcccc caatggattc tttcagatca
    34801 atcggcattt aatagtagat tggttaaatc atattagtca atttatcccg aaagaaaaaa
    34861 aatctatctt agaattattt tctggatctg gtctcattag taattggatt gcaaatcaat
    34921 gtaaagaatt ggtaggttat gagatggagt tagatgctgt gcgttatgca acagacaatg
    34981 ctcgaatcaa tcaaaaatca aatttatcat ttattgcaaa aaatttatac aaagatcgaa
    35041 tttcaaagaa acatttgcaa tttccttttt ggattgtaaa tccacctaga aatggtttag
    35101 ggaagttaat aatagagcaa atactcgagg agaaaccgga aagcttactc tattctagct
    35161 gcaattatgt tacacttgct caagatttaa agaagattgt tagttattac catctagaga
    35221 aaatattttt gatcgatttt ttcccaagaa cagcatactt tgagacaatt ttaatcctga
    35281 aaaggatcta atctttttct cttgccaaaa aggattgaat tcaaatttaa ttaccattaa
    35341 acagaagaaa tcaaatgagt ctcagaaatg acaacgaatt taacaattga aaatcatata
    35401 gcaacgatta ccctaaatcg accagaagca agcaatgcag taagcaaaga attactttct
    35461 tcctttttaa atcatctaca agaagttttc aaccaaacct cattgcgtgc actatttatt
    35521 gaaggaagtg gagaaaaagt tttttgtgca ggagcagact tgaaagagag agcttctatg
    35581 tctgaaaaag atgtattaca ttttttagat caatttaaat ttacttgttc ttttttggaa
    35641 aatcttccta ttccaacgat tgctgtttta aatggcgatg catatggtgg agggttagaa
    35701 attgcactct gttgtgatat gaggctaatg gcaaatgaag caaaaatagg tcttactgag
    35761 acaaagctag gaattattcc tggtgctggc ggaacacaga ggctttcaag gttaattggc
    35821 gttagtcgag caatggaatt gatttttact gctagaagaa tcgaagcgga aaaagcctac
    35881 caattaggta ttgtaaattc aatccatcct aggagtgaaa ttcaaattgc aaagtcatcc
    35941 ttagcagaag aaatattttc ctctgctccc attgcagttc gtatggcaaa aaaatcgatt
    36001 cgaaatggct ttacacaaga tttagcactg gctcttgatt ttgaaagagc ttgttattta
    36061 caaactttaa aaacgaaaga tagaaatgaa gcacttgcag catttaaaga aaagagaaaa
    36121 cccaatttta taggagaata aattgatatt aactggaaaa gaaatttcaa aaaagctagg
    36181 gacggacatt aaaattgagc cttatgagga aaagttatta aatccaaatt catacaatct
    36241 acgactcttt aatgagttgg tggtatatac aaatatgcca ctagatatga aaaagccaaa
    36301 cccttctgaa aaaattatta ttccagaaga aggatatctt ttaaagcctg gaatcttata
    36361 tttaggaaga acgttagagt atacagaaac gcataatcta gttccaatgc tagaaggcag
    36421 atcctcgatt ggtaggctag gaatgtatgt gcatgtcact gctggtttcg gagacgtcgg
    36481 ttttaaaggt ttttggactc ttgagatttc agttatacat ccattaatca tttatccaaa
    36541 tgttcagatc tgtcagattt tctatcatac agtcgaaggt gaaattactg aatataaatc
    36601 gggtaaatat caagaaaata aaggcatcca agcaagttta ctctacaagg attttacatg
    36661 aaaaagaaga ttttaatttc aatctcagta ttactcggat tatttattct ttcatttcca
    36721 ctaacacgtt ataaaatgaa tgaaaagccg cataacgttt caattccaga aatatctcaa
    36781 ttagatactt atttagaaaa agaagaatct aagtttaaag atattgttcc gggcgccgaa
    36841 aagaaaatat tttgggcaaa tgacaaaaag gaacaaactg actatagcat tgtttatatc
    36901 catggattta gtgcgagtag acctgaaatg atgcctcttc ctgaaatgat tgctcgggac
    36961 ttaaaagcga atattttctt tactcgtttg agtggacatg gaagagggtc tgagcctttt
    37021 tcacacgtag atgcgaatga ttggctaaac gatgccgtag aagcaacaga aattggacaa
    37081 aaaattggta aaaaggttat cttgattgga tcatcgactg gttgcacact tgcgacttgg
    37141 attgcaagcc aagataaata taaagatagt ctatcttctt tgatattcat ttcgcctaac
    37201 ttttatccaa ataataaaat gacaaatctc ttcctttatc ccgctggatt agcatttgca
    37261 aaattagtct atggaaatga acgagtttgg cagcctaaaa atgcaggtat agcaaagtat
    37321 tggacgcatg aatataaatg ggaggcaatc actccaatga tgatcttggt gaattttgtt
    37381 caagatattc caatgagtaa agtggtaaca ccatcgctct ttttatacac tgagcaagat
    37441 aaagtaatag attttaaaga aatcgagaaa aagtttcagc agttaggcgc tacacaaaaa
    37501 aagattaaaa atttaagtca gattccagat catgtaatgg caggggattt attttctcca
    37561 gagtcaactg agattgttta taaagagatt agagaatttt taattgagaa taatttaata
    37621 aaaaagtaat ctatgaactt tcgagtagtc tattttagtt taaatatatt aatactattc
    37681 actcttttgt attgtagttc tgggactggt tctaactaca aagaagtcaa aaaagtagat
    37741 tccttcactg gagaaaatgt tcgagacaat gagatcatta tatcttctta caaggagaaa
    37801 ggtcttatct cagtcacagg cagtagggaa aaatttattt tcttgttacc ttattctgaa
    37861 gattggattg taaagcagac tgataattct attgtgcaat taagttcgaa tgataagaac
    37921 ttgattgcat ctatctctac tgaaagagga agttttaagg tagatcctga aaaattcctt
    37981 agagaactga aaaataaaac tgagattaga ttaggtatga agctgtatga tacaagagtt
    38041 ctatctgctc ctacaaataa aattttaggt tattatatta aactcgaaaa caaaggtcta
    38101 gaaattaaaa gtgataattt ttggagtatt cgacaaagac cagatgatgt aattttaaaa
    38161 ttgcatttat cgatgttaaa tgtatcagag gaagaaattc gcaaagcaga aaagatctta
    38221 cctattttta tggataatgg atttagaatt ttatccgaag aagatttgga aaaactataa
    38281 acttagaagc cattctcttc taaatatttt ccattctgta tcgtctgtcg tccattctgc
    38341 ataaatagcg attccaaatt tgtcttggac caaatcgatt ggtagagaat ctattcctag
    38401 ggaaactcca cgaattccag aaaaaatatt ttctgcttcg gagtgaaaac tccatctctc
    38461 ttcctcatag gttggaactc caaaaaaaag cgttactttt ccttctaaaa tacttgtgat
    38521 tttgattact tgtcttttaa ctatataagc ataaacccaa gaaagagggg ttcccgtatc
    38581 atacatcatt acacttattt gatcaacgtt atttgcgact tgaatgtaaa aatctgaagt
    38641 ccacaagcct gctctacttg aaaagaatct aagaaattta tcagcgaagg gaaatggctc
    38701 aatctcatct gttgccattg ataaaatctt atcttgcttt tttgtaatag tatgagtctt
    38761 ttttaaaata tcaagtaggg attcatctcc actatagata ggttcgatgt tataatggat
    38821 tccatcaaaa cctaaattta aaaatgattc agctgttaga acaatcgatt cccgaatgga
    38881 actttgagtt atatctaatt tgcctccgcc ttttttttct agttgtccaa tccatgcttg
    38941 aatatgaatt gattcactac aatcccgcat tctattcaga aattctctcg catatggata
    39001 tttttcctgt ggaatggaac catcgcttgc taaaggacct acatgagcaa atacatctgt
    39061 tatctttcct gtttgtaatt tagcgcataa cagggagtaa tcttcatctg ataaatattc
    39121 cccgacccat ttatgtccaa tccaaattgc atttttattt cttttaaagg aatgatccgg
    39181 tatataattt aaatatttaa aatattgaat aaacaaaaac tgaaagcata ggattgaaat
    39241 cagaagtgaa attttattta tgttgctttt ccaaaaggat ttcattcttc ctctgtctct
    39301 tctttgttta aagcatcatc ttccatgaga tcataagttg ttaagcccaa gataaaatct
    39361 aacgcttccg aaaagttaat tccaatccga gctccaattc ccaagcccaa tgaaaattcg
    39421 acgttaaccg gcgcatggca aagatttggc aaattagcag atggattaat aaaatcataa
    39481 ttattattat aattgtttgg atttgtataa tattgattgc tcctccatct accttgtagg
    39541 ctggcaggaa gaataaatag aaaatttgca tgagtgtatt tttttctcat agatctagat
    39601 gttttatatg cttccggaat atgttcctga ctattcatca aaaagaaaga attgcccatt
    39661 ttgagttttc ccagttttcg atttcctcca gttcggtaag aacctatata tccatttcgc
    39721 attccaagtc cttttccatc ttgggcaatt tgtaatcctc caccaaagca ccagagccag
    39781 gcacttgcac cgtaagtatt agcctcgccg cctatcgtta ctatatcaga aagatcgaat
    39841 gctctattag aaagataatt ttgaatagat acacaattgg ataatgaaaa tagtaaagga
    39901 ataaatagcc atcgaatctt tttcataaga tttcactagt tcaggataga gactagattt
    39961 tcttctaatt catagatata atcaatatta tccgttgcca tataaagttg aacagatcct
    40021 tcaaaaattt gaatgattaa aacggccatt ttctctgctt gggctttatt tgcagcttga
    40081 gaacgaatta aaaagtttgt gatggacttt ttccattttt gaatcattga cttgatatga
    40141 ggtttaaata cttcctcttt tccgaaagtt tgcaatgtga tattgataaa aggacaccca
    40201 tggtattttc gatttttgat ttctacacgt aagaaacgaa tccacgttct cacaaaagat
    40261 tcataggtcg ggttattttt cattaaccgc tccatgaaag ccaaaaattc tgtttctcga
    40321 atttttatgt attctattcc tagattttct ttcgatgaaa agtaattata gaaacttaac
    40381 ttgaaagttt tagattcttc caagatttga ttgattcctg tattgctata cccctgtaga
    40441 taaaataatt tagaggcggt ctccaggatt cgttctttgg gattatcctc ttttgaacta
    40501 attctctttg ccataccctt tacctatttt aacctccgtt cgatgtaaca aaaaattgaa
    40561 aagaaccaat caacaacgat taattggaag aaatttacaa ccatcggtgt tagagaagtg
    40621 atctctagtt gtcagttctg aaaaactctc acatcgaatc acgaaatttt attggaaatt
    40681 actattctat ccgaaggctc taatttgtct ttaaaaaaat attagacaaa ttagtctaat
    40741 attttaatgt agttttagac caattagtct aatcgaggat ttatatgaaa tcaccaaaac
    40801 aaatagcact ttgctcccca agaaggattc catttgcaca aattgggaaa tccttatcaa
    40861 attattcagc tcatcattta ggaaagattg tagcggatga tataattgaa aaaacaaaac
    40921 taccattgaa taaaattgat ggagtgatag taggggaagg atttgcaaat gctccaaatt
    40981 cagccagagt cattgcaaat ttaatcggac ttccaaatga aattccttgc ttaaccgtag
    41041 ccaataactg cgtttcagga atggaagcgg ttgtagaatc tgctcgtaga attttattag
    41101 gtgagggtga attgttttta gctatcggag aggagtctca aacatcaatg cctttcgtag
    41161 ttaaaaatgc acgtatcaac aaaaagacag ctagtttaga taaactcaaa aaagctctaa
    41221 gcgaatccaa ccttccagaa ggggttgaag tgagagatac attagaagat ggtcttggcg
    41281 atggagagac tagctatggt atgcaagtta cagctgaaat cgtttcccaa aattatgctt
    41341 tatctagagc gataaacgat aaagttgctt ttgaaagttt taaaagagcg ttagaggcta
    41401 cagaagctgg tctttatgat ccttatatca tccctgtaaa agatgatgaa ggaaacatga
    41461 tgaaagcaga tgaggcagtt cttcttagaa aaggtctcgt ggaaaatcct gatagaatgg
    41521 gaagagctat gcttctcttt gataatccac aaatgaaatt cacagaattt caaacaaagt
    41581 atgcaaaaga tttaaagaaa actcatgcac ctacagtgag tatttttaat gcaagcccaa
    41641 gatctgatgg tgctgctggc gtgattgtta ctacagtaga aaaagcaaaa gaactcggtc
    41701 ttcaaattga tgcggtttta acaggctgga aaatgaaagg tgttcatcca aatgagatgg
    41761 ggcttggaca agctgcggct actcttggtt tgctacaaga tatgaatgtt aaaattcagg
    41821 atgtagatcg tgttgaaatt catgaagcat ttgccgcaac agcagtaggt gcgttagaag
    41881 aaatcaaaaa tcaaaccggt tgggattggg aaaaaagttt tgatgagaaa aaaatcaata
    41941 cctacggtgg atccatttca attgggcatc catttggagc gacaggaatt cgtcttgtag
    42001 caaatgccgt gatggatatt aaaaatgatt cttctgtaaa taaagttttg atcactgctt
    42061 gtgctcatgg tggaattgcc ggagccttga tgatcgagag atacaaagga taattagtag
    42121 ttattctact ccatcttaac tttatttgag ttaaggttaa tactatgtta ccccccctat
    42181 ccataaagga agggggtttt ttcttaggaa atgatccaca aataaaaact ggggtgatgc
    42241 atgaaaaaaa atcctttcga acaaaagaaa cgttggttgc caaagtattt caaggtaact
    42301 tactttcgtc tgcttttaaa tttttatttt ccctaccttg gtgctggaat aacagtaacc
    42361 catatttcga aagacttccg aagattcgat gtcaaaatgc gactgagagg atataaccgt
    42421 aatttcgtgc aaactcattt tggcggttct ctttatgcga tggcagatcc tttttatatg
    42481 tttattttaa tggaaaattt aggagaaaac tatttagttt gggacaaggc agcaagcatt
    42541 gaatttgtta agcctggaac cgggacagta tatgcaacct ttgaagtttc taaagaagaa
    42601 attgaatcta taaaacagga agtcgatata caaggaaaat taaataaaat atttcatgca
    42661 gagattaaac tagaaaatgg agaagtcatt tctcgtgtcg aaaaaacttt atatattcgt
    42721 aaattaaaat aacatgaatt ggatgtaaaa tttttttcac caccgacttg cactgaacca
    42781 tgtcgaatgc gtctaccgat aactgatttg gcttaaggat ttgcacctgt tacaaaaaga
    42841 ctatttggat ttgttccggg agtgtaagaa gtatttaaag ttatatttcc agaatttgga
    42901 tcaattgtat atacagtgat actatttgaa ttatagctgg aactataagc gaatttgcca
    42961 gacggatcta caaaaactct gttgggagaa acgattccat tagttgctaa tattcctgta
    43021 gaaatagctc cagtcaatga gtttatagga tttgaggcaa ctgtccctag aggaccagtc
    43081 gctgtgtaag cgacgtataa atatttaccg ttgggatgaa caaagacaga ttcaggagaa
    43141 tttgcaaatg ttaaaaaact tcctacagtt agagttcctg taacctgatt gattgaatag
    43201 ggtgtaacct ggtttacatt aaagttcgca acatataaat atttaccgtc aggcgtgata
    43261 gtgacactac gagcatcatt ccctgtagct aatgggcttc ctacttgtgt caaatttcca
    43321 gaagcagagt caatagaata aatgtaaata tcattagacc ctccattgac tgcataagca
    43381 aacttaccat atgggtctac tcgaatccaa cgagttgtag ttccactcgg aatagaattg
    43441 atagatgtta ataatcctgt ggcagtatca gctttgtaaa tcgagatggt tcctgcgccg
    43501 tttgcaacgt atacaaattt acctgtagga tcaactgtta ctgaatttgg atttgttcct
    43561 gtagataaag gggaaccaga tgcggttaac gcaccagtcg tattgttaat caaaaaaagt
    43621 tggatattat tactcgaatt tccgaccgca tacaaatatt ttcctgaagg atcaactgcc
    43681 acatctctag ttccagttcc gaagcctacc gtcccgcccg ttgtcaaatt tcctgtattt
    43741 ggatctatgc taaaaatgga aatattattc gaagaaaaat ttgggatata gataaactta
    43801 ccgactccaa acaaagatct aatttttagg tttgtgctca tgctccctaa agcgttggtt
    43861 gctgtaattg aatagtctgt aagtgtcgtt ccaactgttg gagttcctgt gattgtgcaa
    43921 gttgcattat caattattaa tccggcaggt aaacttggag ttgtcgcaca acgtgtaatt
    43981 gttcctgtta aagatggaat gattggagaa attgaaactc cctgtgtcat tgtatatata
    44041 ctttgagtat aggtaaatga agtgatagga gtaatggaag tattgggatt tgaattatta
    44101 gtagttgtag gaattccaca taggggcgtt ttatcaccca aaataatctt agatagaaaa
    44161 gctctttgga aaaaagcaga attaaaatca caaggattac tatatttggt tcgattgcag
    44221 ttagcgcata ggaataaaag cagtagcgat gaaatgaata aacttaattt caagtgattt
    44281 aaaaatttga agaaatttac gatagtttca attctactca ttaacatgtg gaatgatatt
    44341 tcaaattcta aatttttcaa ttaaaaaaaa gaccgagaaa tgtgtatttg gaaaaatacg
    44401 ataggtgaat atgaaaattt atagcaacta ttggtctttt tgtttcgaaa ggtttgttat
    44461 gagtttaaat gaggaaaatt agatttttta ggtatcttta ctcaatagtt tacaatgaat
    44521 atatttctta tccgatatca atttctcgca ttatttttaa atgagagttt gctaactttt
    44581 gtaaattgta agttcttgtt cgaattctgt tgactttatt taaaaatgag gaactggtgg
    44641 gcagaggagg attcgaacct ccgaaggcag agccagcaga tttacagtct gatctcgttg
    44701 accgcttgag tatctgccct tattattctt agaagtgggt tacttaaaaa aaaattagct
    44761 gcctaaggga atcgaacccc caaccggctg attacaaatc agctactcta ccaattgagc
    44821 taaggcagca tggttaagac cataatttcg caaaactata tttgttcaag aaaaaatggt
    44881 aataatgaca gaaattctca cttttctctt aaaaaaatat ggttgcttgg gtgggaaaaa
    44941 tgaaacgagt agatttattt gtttttatct tgaatagaaa gcattttatc tcgaagcata
    45001 gttgcttttt caaagttcaa ctctttcgct gctgcaagca ttgcctcttt taatttttct
    45061 cttagttttt ctggtgtttt atatttcttt aaagtaaatt ctctttctat ttcattgata
    45121 gtttcttctt ctgcggatag ttctgctact tctcttggaa ggatgtcgtg aatttctcga
    45181 ataattgttt ttggctcaat tccaaacttt tgattgtgtt ccgactgaat tttcctacga
    45241 cgatttgttt cctcgatcgc tttcttcatt gagtcagtga gtttgtccgc gtaaagaatt
    45301 gccataccat tgatatttct tgcggctcta ccaatcgttt gaatgagtga cttgtaattt
    45361 cttaagaaac cttctttgtc tgcatctaaa atcgcaacaa gcgaaacctc tggaatatct
    45421 aatccctctc ggagtaaatt aattccaaca atacaatcat aaattccctt tcttaaatct
    45481 ctaatgattt caactcgttg aattgtttct acttctgaat gtagataggc aactttcacc
    45541 ccaacttctt ttaaatagtc agttaaatct tcagacattt ttttagttaa agtggtgatc
    45601 aatatccgtt ctttcttttc aacacgagca ttgatttcaa ttagtaaatc ttccacttga
    45661 tttgcaacgg gtcttacttc cacaacagga tcgagaagac ctgttggtcg gatgatttgt
    45721 tctacttgta tctctgaatt tttaatttca taatctgctg gtgtagcaga tacatataga
    45781 atcttgtcgg tcatcgtttc aaattcttgg aagttgagcg ggcgattatc taatgcacta
    45841 ggaagtctaa atccaaattc gactaacgtt tcttttcttg ccctatcccc ggcatacatt
    45901 cctccaattt gaggaatcgt tacatgggat tcatctacta taagaagaaa gtcctttgga
    45961 aaatagtcaa ttaaacaagc agggcgctcg ccctcatttc ttcctgtcaa atgccttgaa
    46021 taattttcta ttccattgca gtatcctaat tctgctagca tttccaaatc ataattcgtt
    46081 ctagattcaa ttctttctgc ctctaaaagt ttacctttct ctttgaaaga tttaatttga
    46141 atagcaactt cttctttgat gtttcgaatc gcatcttcta cttttggacc agatgtaata
    46201 aagtgcttgg ctggataaat caccattgtt tccattttag aaatcacttt tccagtcaat
    46261 ggattgaaac gagataagga ttcgatttca tccccaaaga attcaatacg taatccatcc
    46321 tcatgatagg caggcattaa ttctaaagaa tctccgcgga ctctaaaatt tccgcgagaa
    46381 aaatccaaat catttctttg gtattgaatg tgtaatagtt ttttaattaa tagatctctt
    46441 tctaaagttt gtcctcgttt taagatgatt cttgattcag tgtaatcttc tggcgaacct
    46501 aaaccataga tacaagatac agagcttact atgattacat cttcccgctc taagacagaa
    46561 gacgttgccc gtaatctcag tttgtcaata tcttcgttga tggaggaatc tttttcgata
    46621 aatgtatcag aagagggaac ataggcttct ggttgatagt agtcataata ggatacaaaa
    46681 tattcaacag aattattcgg gaaaaattct ttgaattcac gatataattg agcggctaac
    46741 gttttattgt gagacaaaac aagagttggt cgctttactt tttgaattac ttcagccatt
    46801 gtgaatgtct taccagagcc agtaactcca accaaagtaa cttttttctt tccttttaga
    46861 aaagagtctg aaattttttg aattgcttta acttgatccc cagcagcttt gtagggagta
    46921 actaattgaa aatctggcat tgatttaact tagttttttt tctgcgtata gatgtgcttg
    46981 cttcttgtca tcgatgattt gtaattctaa atgattaaat aatttttgca taaccataat
    47041 acctctcata agagttgtat tagaagaata ctttcctctt tcaatttcat ttgtcagatc
    47101 aaattctttg atattgttaa ttatatttaa ttttaaaact cgattcgaat caatcatgta
    47161 atcaatatga actatattcc catcaccata cttcacagca ttttcaattg cttcaatcgt
    47221 agcaattgat atgtccataa tgtcttcttc cgttacataa ttgtattcta aaaaatattc
    47281 ttgtctactt cgaacatatt gaataggaga aaactttgca tcaccataaa cagaatagct
    47341 gtcagataaa taatcagttc ttttgggtat tgcgttaggc gtaatgcaat attcagattt
    47401 atcgaattta ccttctcttg caaaacctga ttcctttaag gaatctagta tagccttaac
    47461 tttggactct tctacagaaa tctcttcatt gtattttaag acaccttcta gcgaaagcca
    47521 tgaacagtaa tctttgacag tgcctgtaag cccttcggca aggatatctc gtataagttt
    47581 ctctaaaagt atgtcagtaa tttccaaagg catggttggt ttacaaggat gtacgataat
    47641 ttaattgtgt aaagtgttta tttttaagat aattccgtga gtagaaaact aagctcattt
    47701 caaatactct aattgcaaac tcttatctta gggtgactga tgaagcaatt aggaaggaga
    47761 tgtcaagggt gataagtcaa aatagtctct tttagaaaag aaaatctagt taaaatctaa
    47821 atttaactgt cttacggctt cataggcgat aactgcaact gcattgctga ggtttaggct
    47881 tctacattcc tttgccattg ggatataaat ctgattatcc gctggaaatg cattccttat
    47941 ttcaataggc aagccagtcg tctcccgacc gaaaacaaaa aaatcatctt tctgataggt
    48001 tttagatgaa taaagtgact caccaaactt agttacaaga aatactctcg agaggtctgg
    48061 aatcacttgt ataaagcttt cccaatcttt gtgttgatat agtgtcaaat gttcccagta
    48121 atcaagtccc gctcttctaa ctgctttttc ggataagtca aaagaggctc ctcccacaat
    48181 gtgaagattt gcctttatac caacacagag tctggcaata ttccctgtgt tgggtggaat
    48241 ttctggtcga aacaaaacaa tattcaagat taaaaccagt ttttattttt tcagagactt
    48301 cttaagtaag agtccaaaac tagattcact tgtagatttc tgttccgata tgtaattttc
    48361 atagtcgata cgatctttag catctctcgc tttagacaat gaaacggcaa tttgcttttt
    48421 ctctggatta acttctgtta taaatacctc cagtatatct cctgctttaa aatgatttga
    48481 tagagatgtt cttggaggat agcctgtttc cttagttggt aacagtgcat gaaagtgttc
    48541 atttaacttt acaaaaacac caaagttctt aattgaatca acggttgctt ttacaatgtc
    48601 tccttcttta aaaggaacag cttttgccca tggatcgttt gaggaatctt tcaatgaaat
    48661 agaaaattta ttttctttcc agtccattcc tatgatttta cctcgaacgg ttgcacctac
    48721 agtgaattct gtgtttaagt ctacattttt tttaaaactt gcttcactaa caggaactaa
    48781 cgcatctatt ccattcatat caacaattaa accaaaatta tgtattgatt taactttgca
    48841 agtaacgtaa gatccttctt tcaattcgcc tttcagtatt tctcttttta agtccttctc
    48901 tttgtctgaa atcttctttt gagacaaaac gatcttttta tttttcatag aaagatcgtt
    48961 tactatgaac tttaattttt taccttgcaa ggcttgagat tttgtctcgg gatctaattg
    49021 ggaataagga caaaatgcgt tatactctcc gattttaaca tcatatccac cagtagtttc
    49081 tccaccaacc tgaccataaa cggggatttc atgctccaaa gcaatttcaa gattttcagg
    49141 ggttatatct tctcctgtta aaacagttgt aaaataatag tccccatgat tttctttgag
    49201 gaaataaaca gagtatgttt ttccaggctc tagtttattt tcaccttcga taaattctgc
    49261 attagagata attccacgaa tgctatcagg ctcagttcga atgaatgtgt aatcactctt
    49321 aacggatgaa acgattgcat tgtattttgc tcctggttca agactttttc tttttttaaa
    49381 actctcttcc agtaatgtcg caaagtcatt cttattcgcc atattgttaa acctacttat
    49441 ttttgttgtg ctagctgttg tagttcttgg attcgcttgt ttttatcatc actttcccaa
    49501 acttttttac taaattggga tgcggtgtaa gccttagttt tgtattcttc cataaaagga
    49561 acgattaact ctttgtcagg ctctgtagtt tttgctgctt gaattttctt taatccttct
    49621 tcgccttgtt ctaaccactg aggatcaatt ggtaaattga ctctatatcc acgaacgtta
    49681 gtcattacat aaggaccatc ataacctttg tctctgagaa ttttaccgaa gaataaaaat
    49741 tctacatcat ttgttccttg ttttaaagga ccatcaaatg ttgcatatgc gaggtagttt
    49801 ccttcttttt cttctttcag gtttgcttca agacgatagt taccagcttt ataaacaata
    49861 acaggagcat gaattactaa agaaccatca cttagagtat ctctaaatgt gtttccaatt
    49921 tctgcaggct tgtttggaga tgagaaaaag cttgttgtta actttgcttt cttatctttt
    49981 gcgtaagtga tgttagcttc tagtgacatt tgtccccaat cagctttcat tggcttccat
    50041 ttaaatgtga cgattccatc tttggcaact tcatccccat ctcttccgtc atcattaaaa
    50101 tcagggctat gagttggagt tttttggtct tcgaactctt tgtagacagt tgcatttact
    50161 acattcactt taattaaatc atttgaagaa tctctacatt ctagtttgat tatcatatca
    50221 ctgttagatc caatcacagc ccaagtttta ggttgtagat gacatctgta tccattgcct
    50281 tctttgtcat ttggtgaatc taccattgtg attgcattga tatcaatttt aaaagggtga
    50341 atgatatctt gattcaagga agaaattggt cttgaatgtg gaggatattg tgcccattca
    50401 gtatattgtt ctagtaaaaa agcgggagta actccatcac tgatttttcc cggtccactt
    50461 tctgaagtag ttgttgtttt atcttcaaaa ttttcagctc cactctttgt tggatcgctt
    50521 gattgagaca ttggatcgcc ggatttgccc tttgagcttt tttttccatt tcccaaaaat
    50581 agaaatagtc ccaccgctac tactaggata ccaattacac catatagaat attcttattc
    50641 ataaatcctt cctgtttttt taatcatttt gaaaataaaa attctctaat gttaacgttc
    50701 aaatcttttt tattttttta ttgatttctc tgtccaatag cctaatgtca gaaacagatt
    50761 tcgaaaagaa atccaaatag gagaaaagaa aaatgttaaa atcgaaaata aacttattag
    50821 ttgtagctgt atcccttgcg gtatcagctt ctttgtcagc gggaacaatc acagttttcg
    50881 tacatgggaa gtctaattca aatcacaatg gaacaggaac aactgatgta aacaattact
    50941 ggggagcaag tgcgaataca gtaagtggcg taaaatattt cgtaggttat gatggaacat
    51001 ctgatccaag aacttatgga actgcaagag cacaaacaaa ccttacaact gttttagtaa
    51061 accagtgtaa aggaacaaat acttgcaaaa ttgtatgtca cagtgctggt tgctatgcaa
    51121 ctgagtattg gttatctaac ctaggtggaa cagcttctag caaaggtttc aagatctctg
    51181 gtgttacagc gttagctgct gcatctggtg gttctgaact agcaaactca ctcaatgcaa
    51241 tttcatttgg ctatgttgga aatgctatgg ataaagctct tatcgttact aatgcaagat
    51301 cttctttcaa tcataacaac acagctggtg ttacagttgc tcacgttcct ggttctaaag
    51361 gaatgattgg tgcgtctgca attcttccag gagaggatga ctatgctgta gcgtatcact
    51421 cttcttgtgg ttatagcact gtaggtgggt tatcaaaatg ccaaagctca attactcaat
    51481 ctgagggaat ttggccattt aactcaaatg taacttatac acagtatctt ggtcacacga
    51541 gagcaccttc tgttccagta gaaggtctat acgaaaatca ctcagaatta acaaatgatg
    51601 gttggagata gtccctagtt tttaaagaag agacagtctt aagctgtctc ttcgctatat
    51661 ttaaatcttc gaaatttttc ctatataaaa agaatttgca atctgcaaat cctacctact
    51721 aaaactcaag ttatccttta taaaaagtag cggctaataa ttgtaaacct ttgttttctg
    51781 attgatgttt gaatcaaatt caagctattc tggtaattga gaaatataaa agaatatgga
    51841 gaaaaaaaat gcttaaatca aaaatcaaac tatctttact tgttgcttcc ctctttttgt
    51901 ctgcttccct agcagcagat acaattaaca tctttgttca tggaaaatct agttctaatc
    51961 acgatggaac aggaacaaca gatgtgaata actactgggg aacctctcca aattcagtga
    52021 gcggagtaaa atactttgtg ggctatgatg ggacaacaga tccaagaact tatggtcctg
    52081 ctagagctca aacaaacctt acaactgtat tagtaaatca atgcaaagga acaaatactt
    52141 gcaaaattat ttgtcacagt gctggttgct atgcaactga gtattggtta tctaacctag
    52201 gtggaacagc ttctagcaaa ggtttcaaga tctctggtgt tacagcgtta gctgctgcat
    52261 ctggtggttc tgaactagca aactcactca atgcaatttc atttggctat gttggaaatg
    52321 ctatggataa agctcttatc gttactaatg caagatcttc tttcaatcat aacaacacag
    52381 ctggtgttac agttgctcac gttcctggtt ctaaaggaat gattggtgcg tctgcaattc
    52441 ttccaggaga ggatgactat gctgtagcgt atcactcttc ttgtggttat agcactgtag
    52501 gtgggttatc aaaatgccaa agctcaatta ctcaatctga gggaatttgg ccatttaact
    52561 caaatgtaac ttatacacag tatcttggtc acacgagagc accttctgtt ccagtagaag
    52621 gtctatacga aaatcactca gaattaacaa atgatggttg gagatagtcc ctagttttta
    52681 aagaagagac agtctcaagc tgtctcttcc taaatctgct ttttaacttt attcttattg
    52741 tttagctgat tcaatttatt acatacctga gtgaatttga aagttctgac ttaaaaatga
    52801 aacagaactt tcaatacttc caaatattca ttttttaata tttgtatctc agatcatttt
    52861 agatataaaa tttatcattc ccattggttt atgaattttg ttctttaaac tttacagaag
    52921 gaatttctag gactaatttt agcataaata gaattgtatt tcgattttat attgcagtgc
    52981 ttttttttct tctagtttgt tttttgattg atagtctgaa attgctttct taacgttggc
    53041 tgtgtgaatt tcgaaaagaa attctaaata ggagaaaaga aaaatgttaa aatcgaaaat
    53101 aaacttatta gttgtagctg
//
LOCUS       DAOQOS010000023        51937 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_248_length_51937_cov_5.957712, whole genome shotgun sequence.
ACCESSION   DAOQOS010000023 DAOQOS010000000
VERSION     DAOQOS010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 51937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 51937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51937
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_248_length_51937_cov_5.957712"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..218
                     /locus_tag="PLF66_07010"
     CDS             <1..218
                     /locus_tag="PLF66_07010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63825.1"
                     /translation="LPWYAKIKSISLKKREEILPFRNPNTTPTQGVSHYYLVELETPM
                     DMSPIKAIDDLEIGKPIVKKWNEILQI"
     gene            237..446
                     /locus_tag="PLF66_07015"
     CDS             237..446
                     /locus_tag="PLF66_07015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63826.1"
                     /translation="MMKTEILDSNRIIYQLRVNDIASVIKEDEMDLELTEDLVLHLEK
                     RMSNYLDWSEAIRFALADYQNKEEL"
     gene            443..700
                     /locus_tag="PLF66_07020"
     CDS             443..700
                     /locus_tag="PLF66_07020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63827.1"
                     /translation="MKYLLFVNFENNLKHNSVFETIEEVIESAKHIIKDEELNEELEK
                     FPETKDLINHLNTNDDFLFTLSNGTWFPIQSYPVFSRHFQL"
     gene            complement(812..1006)
                     /locus_tag="PLF66_07025"
     CDS             complement(812..1006)
                     /locus_tag="PLF66_07025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63828.1"
                     /translation="MKNTKKEKDRERCNQCGDSVAFGSGKFVNRIPDLNDISTRRSMN
                     RKYIWGDFVCIECDEKNSDS"
     gene            complement(1220..1396)
                     /locus_tag="PLF66_07030"
     CDS             complement(1220..1396)
                     /locus_tag="PLF66_07030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63829.1"
                     /translation="MNSLKNYIDEALRVTGNRIEEMTPLESKFFREKVLNQFSQKKPF
                     KPLWQKLYEIFLFL"
     gene            1614..2129
                     /locus_tag="PLF66_07035"
     CDS             1614..2129
                     /locus_tag="PLF66_07035"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HMZ63830.1"
                     /translation="MKLTVEDGFPYYIELVGEKNWKEIYKAKESINLFEKIDEEKANY
                     RFESGKWSIKQIVGHLTDHERIMTYRILRFSRKDETLLPGYDQETFIQNSNFEKIPFA
                     VVLEDFKNVRNASISFINTLSEDQYKLKGKAWKYELTIEEFLKATIGHEMHHLNVLKE
                     KYLRITTDAHR"
     gene            complement(2285..3025)
                     /gene="eutC"
                     /locus_tag="PLF66_07040"
     CDS             complement(2285..3025)
                     /gene="eutC"
                     /locus_tag="PLF66_07040"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015679158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008851 - ethanolamine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutC"
                     /protein_id="HMZ63831.1"
                     /translation="MPQKEITKNLDPWTKIKQHTAARIGIGRSGVGIPTKELLQFQMA
                     HAKARDAVLTEINFSEIKNWLRKKYKFQELESACESREEYLTRPDKGRILSDSSKKNL
                     SKKKTKSISICIVDGLSSSAIEKNSIPLLKILNPKLEELGFTLSFSLVKNGRVAIGDE
                     ISSLEQTDISLVLIGERPGLSSPDSLGAYLTYKPKKGFTDEKRNCISNIRIGGLDYKL
                     ASDKILYLVTESYSKKLSGVNLKDNLIA"
     gene            3171..3653
                     /locus_tag="PLF66_07045"
     CDS             3171..3653
                     /locus_tag="PLF66_07045"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HMZ63832.1"
                     /translation="MVISKNKVVAIDYTLRNSEGEIIDASAGQEPLVYIQGIGNLIPG
                     LEEMLEGKKVGDTFKAVIPPEKAYGEFDESLVQLIPSKHFAQIPNLEVGMQLQANMDG
                     QIRIVTVTAIEENSVEIDANHPLSGETLDFDVTVKSIREASQEELDHGHVHGEGGHHH
                     "
     gene            complement(3650..5017)
                     /locus_tag="PLF66_07050"
     CDS             complement(3650..5017)
                     /locus_tag="PLF66_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020769577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutB"
                     /protein_id="HMZ63833.1"
                     /translation="MYKTTVSKKTYQFKNLIVLMAKATPHRSGDVLAKVSASTQEERV
                     AAQITLSEVPLKRFLEEELIPYEKDEVTRLILDSHDKKAFQIISGLSVGEFREFLLSE
                     NTTTETLTDLQDGLTPEMVAAVSKIMRNQDLISVAKKCNVVTKFRNTIGLSGRLSTRL
                     QPNHPTDDLKGIAASLIDGLLLGSGDAVLGINPATDNVYNAIELLKMLDEVREKYKIP
                     TQSCVLTHVTNTIEAIQKKAPIDLVFQSIGGTEKTNTSFGINLKLLQEAYEAGLSLKR
                     GTVGQNLMYFETGQGSALSANANHSLDQQTCEVRAYAVAKKFNPLLVNTVVGFIGPEY
                     LYDGKQIIRAGLEDHFCGKLLGLPMGCDICYTNHADADQNDMDVLLTLLGVAGCTFIM
                     GIPGADDIMLNYQSTSFHDALYARQVLGLKPAPEFEDWLIRMQILDEKMIPRSKTPAK
                     LLADY"
     gene            complement(5010..6320)
                     /gene="eat"
                     /locus_tag="PLF66_07055"
     CDS             complement(5010..6320)
                     /gene="eat"
                     /locus_tag="PLF66_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002770707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine permease"
                     /protein_id="HMZ63834.1"
                     /translation="MSKLEKNLGPFLLWGLGVGYVISGMYFGWNLGLPRGGSFGFLMA
                     TLFIIVLYITFTFSYTELACAIPRAGGAFDYANFTLNHHFGYIAGIAQIIEFVFAPPA
                     IAVAIGAYLNLFFPSIPILVAAIGSYFVFTALNIFAVKMAAFFELIVTILAVSALLLF
                     AAIALPHFQFSNLNKNPLPNGISGIFASLPFAIWFFLAIEGIANVAEEVINPQKNILL
                     GFGFALLTLVFLCILTFFSSIGINGWESIVYISGTKEMSDSPLPLALASIIGKNSFYY
                     QIIVFVGLLGLIASFHGILLASGRATYEFARVGYAPKVFGNINARYHTPANALLGNAI
                     LGILALLTGKTAEIITLACFGALTLYIISMVSLFYLRKDHSHIKSSFRVPFFPITPFI
                     AFLFAIIALFSLMIFHFEIGMIYLFTLTLAYSYFYFYLKDKKHV"
     gene            6680..7207
                     /locus_tag="PLF66_07060"
     CDS             6680..7207
                     /locus_tag="PLF66_07060"
                     /inference="COORDINATES: protein motif:HMM:NF019235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1564 family protein"
                     /protein_id="HMZ63835.1"
                     /translation="MSENLNGKTFSKQKILFPRENLKYAEFSSTLLIPIEYLDLLQSK
                     IIKHKGIRSYVAYLLGKYQLHIANGLVPGYSNVTTKYQEKDLSLQKISFRPRPEDWAE
                     LKLYRISFGMSISAFFVYLLIADSVDFAETVSHYLSTVGIPVIANFDLTAKVYLWNQK
                     TFYTKVFLYRKGKYY"
     gene            complement(7239..8177)
                     /locus_tag="PLF66_07065"
     CDS             complement(7239..8177)
                     /locus_tag="PLF66_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002972517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HMZ63836.1"
                     /translation="MSKRILVTGGAGFIGSHLCERLLKEGNEVISVDNYFTGRKKNIA
                     HLLKNPNFEMIRHDIINDLRIEVDQIYNFACPASPVHYQYNAISTVKTNVMGTINMLG
                     LAKRTRARILQASTSEVYGNPLEHPQKESYWGNVNTIGIRSCYDEGKRISETLCFDYN
                     RQHAVDIRVIRIFNTYGPRMLANDGRVVSNFIVQALSGKDITIYGDGSQTRSFCYVDD
                     LVNGIISMMNQDSFKGPVNLGNSDEFTVKELAELVIKLTKSKSKIIYNPLPQDDPARR
                     RPDLTLAKEKLGYKYNVPLIEGLKRTIKYFQGEIKR"
     gene            complement(8170..9024)
                     /locus_tag="PLF66_07070"
     CDS             complement(8170..9024)
                     /locus_tag="PLF66_07070"
                     /inference="COORDINATES: protein motif:HMM:NF024790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HMZ63837.1"
                     /translation="MRSQKLIFFFSIVLTLIAFWDFKIMIHLKAVIVLFHEICHATAA
                     LLTGGVVEKIFIHGNESGETVISGKLQASFLFVVSAGYIGSSLLGGILLNRGFHKKQA
                     KHFLGFVGIFLILFTHFYSSSHDFTYNTGIRFGIIFVILSLTSREVSSLTLIFLGTGV
                     SLYSIYDLADFTNHIHQSDAGILANWMIGKKERFLGLNLNQLGVMIALIWSSISVLLL
                     GTFVKKTFTEPDPFAKMSKLKKQFAQANVEPDVAQWFLSRGLDLDGKPLHKDWATQLK
                     KEGLNEHE"
     gene            complement(9026..9490)
                     /locus_tag="PLF66_07075"
     CDS             complement(9026..9490)
                     /locus_tag="PLF66_07075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63838.1"
                     /translation="MNNFILLFLFCGYLGLSSIVSVEEKNPSPFFYLTAKPIKIKINP
                     KIHQLCRVKLNEVVEYKHVEVLDCYNIDRKLSDSIKTIKEKNPDKNEINMMFVDENKK
                     TLSPDITTGQGNYKVKSLLFFKKTKANHIELEDNSGGYYYFKFEPAVVSIGE"
     gene            complement(9487..10557)
                     /locus_tag="PLF66_07080"
     CDS             complement(9487..10557)
                     /locus_tag="PLF66_07080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63839.1"
                     /translation="MRNKVKKDLKGLVQSFLQINWKLIRLTMRAVLFILLVGIMTSLG
                     SKQIYAKESKSLYNEAYRIEKISPLLAINLYEKVLEQADISKKVQKNTINRLFFLYIK
                     FSRFEEIFILNSKFPPDKVRQKKTDQLVKNISTNLKIDESAFLEMVNISLKNDETSRK
                     DLLSIYNYYPSSYLLHYIFAIKFQTKDLDSLAFLISEIPDINPVLKLAYITKAASVST
                     TATSIEKAILDAASISSLTNEQKSDILFFYGTHLRNKKKYRQSIRYFHMSASYAKKED
                     GFISAEMIEVAKTLFISGGEKESCEILKSGKIKAQKEQDEILNLYCKNKNNFKSVKRS
                     IQELILRNEDDFFYKKMGKLIQ"
     gene            complement(10570..13110)
                     /locus_tag="PLF66_07085"
     CDS             complement(10570..13110)
                     /locus_tag="PLF66_07085"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HMZ63840.1"
                     /translation="MKTLYNERILPDGSFFCESCHSKSKLSTLPNRPGTYKITCYKCK
                     HETLVRVEEENSNQEFILEPEAKAENTPKVDTNKELTEEEYKKYESIFLQDTQFISVG
                     DAVNKPLLEINKVTLPKIKKQETWFEKFLQKFQLPEKTDSNRYLQFAKKYRYFLAAAL
                     LILISISIIIPIRSSYIEVKSEVDELLVELNRHKPSKILDRNGVLVSEIFQKKTGTLK
                     LSDYPPKLIKIILSVEDQNFYKHGGIDYFALLRATYKNIINMGYIQGASTITQQLARI
                     LIKDRRKSIGRKFREAMVAIALESKLSKDQILEAYLNQVYLGHGAFGIENGAKYYFNK
                     TPDKLKTMEMILLSSLASAPNRYSPFKNRELSEGRVRVIVNTLEARNIISERFQNKIN
                     KFYETLETPASMTVFGSRYDKAPFVTEHIREFLKSLDPNINIYDIGGYTIETTLIQEI
                     QEVLSDEVALHLNQMKEKGKIKRVKIKNTPDNTDVSDELQAAVVAVDPSTGAVLFMHG
                     GGEQFNSKNQFNRAVQMRRQTGSSIKPILYASAIDSGIFNAASILLDAPIMFSSPDGK
                     GTWTPDNFGSAYEGEMNLRDALAKSKNTIAVQIGERMGLPNLEKYYSSFFFPDPNEKS
                     KRYRNDLSVTLGSLEISPIEMAAVYGNFSNDGIIKRPYLITRILNQEGKEIYKHFDRD
                     EFQLKIPEERRVIAPDTAEVMVSLMKGSANASGIRKYGYSGEIAGKTGTTNDNIDAWF
                     VGTRPRLSMAIWMGYDDASYGMGKSGMGAELAAPLWARIAKKIKDLNVIPEEKFAFSK
                     HATRQVVCRESGLYASDICPDRVSELFTKEGKPNGTCRLSHDYVPKLR"
     gene            complement(13340..13750)
                     /locus_tag="PLF66_07090"
     CDS             complement(13340..13750)
                     /locus_tag="PLF66_07090"
                     /inference="COORDINATES: protein motif:HMM:NF013440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HMZ63841.1"
                     /translation="MFKNKILITQLDKERILNLLKEGYSLNSNKYLSQVFEKEIKKRQ
                     AVKSDKIPPDVITMRSKFRLKDLGTGQRYEYTLVYPQEVDSESKISILAAYGTAVFGS
                     RKGDVVRWDLETGTKFFQIDEIIYQPEASGHWDL"
     gene            14047..14646
                     /locus_tag="PLF66_07095"
     CDS             14047..14646
                     /locus_tag="PLF66_07095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63842.1"
                     /translation="MEFVNKVRGLYISGRAIRTLVELLGESKQVGVKIMKFHKITDDQ
                     APEKFSINPDEWYDLDRFLLGMRDIQERVGEEKIRQVAYNTIHYAPLPTHIQDIMTSL
                     NHMNKAYLLNLSRDGIQTMLDEPGGKQSEAVGKISVTPVPGIRKVICLSTSCFPTFYE
                     LGLIEGFAKKFQPKATVTLDKTRIQKKQGGDSNTFIVTW"
     gene            complement(14809..15846)
                     /locus_tag="PLF66_07100"
     CDS             complement(14809..15846)
                     /locus_tag="PLF66_07100"
                     /inference="COORDINATES: protein motif:HMM:NF015252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipase secretion chaperone"
                     /protein_id="HMZ63843.1"
                     /translation="MNKKIIIGIVAAALALTLGYLLFGAKDKKKSTSSSQEESNDTVN
                     RSTNDTISPFGDTTSHWEEALSPFQGTEKKGYLELIEDLKTGRVNFVWEVWALRRKCP
                     ADYTALQCDETIYKFIDTKFASPDKEKMKELFKAYFQYEDEARKMEFPANITFQERYE
                     ILKNRRREIVGQEKADLFFGMEESQVAFMQASKNFMDSTKNLSGSERVKKYEELRKNT
                     YGSYLNAVNGREDSFDHYKTEISLREKDLAGLSPEEREKQLTSLQVKYFGKEGAERIA
                     AANKEIAAHDKKIADYEKAEREFLSSNNGISDKDKEQKLRELRVKYLGQEDAEAYTRA
                     KVFEEEAAKIK"
     gene            complement(15991..16914)
                     /locus_tag="PLF66_07105"
     CDS             complement(15991..16914)
                     /locus_tag="PLF66_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015677090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HMZ63844.1"
                     /translation="MKAKFLTLIFTLFFSLPVGLFAGPLDGMCIALVHGILGFDDTQG
                     LAGGLVKYWGGMDTYLRSQGAKVTTPGSSPLNSIQKRSELTRDALIPWMAANGCTKVH
                     LIGHSQGGLVVRYMASNSALGFNTSANKRVATVSTVDAVHKGAPLADIVLGVIPSWLQ
                     PFANTALGLFSQLFYGAKPMDVIAMGKSLAVSTVNSFNAATPNFTGLKYYSYGNKMAW
                     ADPIQHVLMAPTYPITWAGGLFYGLGSANDGVVPLESQKWGNYKGEASAKWFATGIDH
                     LQATNFQWSGQNFFDVTGWYLGIAQTAKAGL"
     gene            17569..18123
                     /locus_tag="PLF66_07110"
     CDS             17569..18123
                     /locus_tag="PLF66_07110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63845.1"
                     /translation="MKKFYLLILALIFVNCAKTEYFTRNVNTQIRMNSFSDEEGYKLK
                     KRFETSHTRINLFWGLVTTINKRDMEWILASELKDPDKEAIGNLTITEYYDFLDGLVD
                     TVLFGLIRPYSVILRGDLYERASNAPIIPEDKQNSEMKEVKDAPLETSDAKSEKDIVK
                     DSDSAKDSSKPSNTKKGEKNVKKK"
     gene            18107..18502
                     /locus_tag="PLF66_07115"
     CDS             18107..18502
                     /locus_tag="PLF66_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004787913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63846.1"
                     /translation="MLKRSKFLLIILMLLFFINCASHTYQINNYKVPVSFANEEGSSK
                     NSRYFRIEKKLTWLIFDLVKIQEFDLTHVLESELPNAKKIYNFRIESEENAIDSVIRI
                     FGTGMQYLFASNRSIVSRRTVIISGTVSE"
     gene            18674..19582
                     /locus_tag="PLF66_07120"
     CDS             18674..19582
                     /locus_tag="PLF66_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002750550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl acyltransferase"
                     /protein_id="HMZ63847.1"
                     /translation="MKQIGYFLSFLFAYFLYLPFKFLPYKACLAYGVGITKVLFPFAK
                     KHRKIAYDNISHAFPEYTHEQRLALVKKHFEHLGRLLAGTLFASRVNKEWLDKYLVYD
                     PESLQIEEDTKKEGVGVVLVSGHLGTWEILVQFMGVRMKGAGIYKQIRNPFVDKWVKS
                     LREKNGIVLVTTEESNKVMKLLKDGYWIGFGSDQNAGKAGIFVNFFNRPASTYQGPAL
                     MAYLTGAKLLLYSVVCGEDGKVIVRVKNMGFIDKKKFPSREAAIRHYTELWTHLLEEE
                     VKLFPDQYFWVHRRWRTKPGDFPGQT"
     gene            complement(19736..20518)
                     /locus_tag="PLF66_07125"
     CDS             complement(19736..20518)
                     /locus_tag="PLF66_07125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63848.1"
                     /translation="MNFKVLLLFLILFNNFIFSQDETPKSNEETSSQSSSSNTDESKK
                     ENDSNQGKNREERKLVVSLSYNASAELRVEYNLNSRFTIGSHFFKDYLRETKENRLVG
                     YTTYQHAEFYNRRGADLYGKFFLFTEGPVYLVLGAGKYLDGGATRNTLLFTRNPTSQV
                     LTTEAISNSTTVNPYWYAKYGVGLQWIFDFGLLLNLEASSLVPIHPSRKSYTYVDERN
                     FISNNYYTPANLYFIDKYLQTKDPNSSGLLQFNLWIGYAFAI"
     gene            complement(20521..21345)
                     /locus_tag="PLF66_07130"
     CDS             complement(20521..21345)
                     /locus_tag="PLF66_07130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63849.1"
                     /translation="MTETIFNLPDANTYFQLMLKTYAKETPCVQATFSVNDDLLLEYG
                     SELEPRSLLLQMTEDLKKFDLLASLLDGQIRFSKIKTIEKENEKYDFYTYSIEKNKNI
                     FRSVFLIPSDKTPNESNFYNLNEFVKNYYMNQPKKIDFQVIDTYSEFLDKVIREISRR
                     FTSKEEVGVVSHFQIQDLKTYHQHMSRQYTEEIHNEIKTGILRHLKKGDLLFRVNQRS
                     YITYSHNCNVDVVKKRFSDIFFQLNTLIIRYNLSFHQSDFNNFKKRDFWDKITSGA"
     gene            22098..26027
                     /locus_tag="PLF66_07135"
     CDS             22098..26027
                     /locus_tag="PLF66_07135"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HMZ63850.1"
                     /translation="MRSAEYPSNEKERLKKLIDYSILDTLEEQEYDELTHLASYICKT
                     PIALISLVDKDRQWFKSRVGLNAKETHRDLAFCAHAILDKEIFVVPDSSQDDRFSDNP
                     LFLNDPHVRFYAGAPLLSPEGLPVGTLCVIDHIPRELSSEQLVALKTLSKNVVNLLEL
                     RLKMKEQEKQIELLSESENQLFQASYKYESLLNSRSVFILRTDLSGKYSFANQYYSDT
                     FQMDPNHILEMDTFSHILEGDHFKVKETIQKCIHSPETPFLVSLRQLQPDGNILYSDW
                     EYVAIKDKNGKLVEIQCLGIDITKPRKAFLDLENSLLKVHNLESAMNEHSIVAITDSE
                     GKILFVNEKFCEISKFTKQDLLGQTHSKVNSKFHPKEFFEELWNTILNGKVWKGEIRN
                     VSKNGGFYWVSCTIVPFLNSEGIPYQYISLQTDITSQKRVEEDLKRSRERYSSSLENM
                     FHGCFLIGYDMRYIYVNQMGAILGRKLKEEFIGRTMSDVYPGIENTYIYQSVLNCMRD
                     KKRTRFETEFHFEDGGIGYFELSVEPSSEGVFIVSTDISNRKILEQQILDQKDFIHKV
                     TNTIPGMIGYWTSDLRCAFANDAYKEWFGMREDIIGKSLEELLGKQIFQKNFVHIENV
                     LKGQKQIFEREPTERAGYTWIQYIPDIVDQKVKGFLVLITDITDLKVTEISLIEARNK
                     LREILEAIPEGLVEINLRGEIIYANQGAGKILDIHRNGVEGKYFNSRAWQQVDQDGNP
                     FPLEKLPLSIAMKEEREVGPIEHGVVDSTGKKKWLSVHAVPLFDKDKKMYGAVASFRD
                     ITERQKFQEMIIRSKEEAELAREMADKANKAKSEFLANMSHEIRTPMNAILGFGELLA
                     SRIMDENLKEFAESITTSGKILLKLINDVLDLSKIEAGKLELNYTPIHIDKILSDMKT
                     IFSQKIEEKNLKLITEIESNVPEYLILDETRLRQILLNLIGNAVKFTDTGLVRVIVST
                     EFKTNQKRKVDLKIQIEDTGIGIPSKDKEKIFEAFSQSDGQDHNRYGGTGLGLTIAKK
                     MVQLMNGEMSMVSEAGKGTMFVLKFLNVSISSIGVEDEDRSIDVKNIFFSKAKILIAD
                     DVKLNRQLLIKFLEDQSELEIIEAENGKIAVEKAVKHKPDIIFMDVKMPVMDGLEAIQ
                     ILKKDPETKLIPILVITASVFKQTRDEVVAISDGFLQKPVSQKKLISKMIDYLDYKRV
                     IREEKQNAVESKTQITIDPKLAMEMYDKIMNDSYHKWLTLKDIMNITEIVKLANEIQK
                     IGEEYKYSPLIFWGKKLEKYANLFDSNKINSTMQQFSDLLKVLKGDR"
     gene            26024..27109
                     /locus_tag="PLF66_07140"
     CDS             26024..27109
                     /locus_tag="PLF66_07140"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HMZ63851.1"
                     /translation="MSNQTILVVDDTPANLQVLGQILSKEKYKIIFAFNGEQALKSLE
                     HQIPDLVLLDVNMPEMDGFETCNRMKQLELLNNVPILFLTARTDIEDIVNAFKIGGAD
                     YITKPFNPTELLARIHFHLSLKEKTEQLKEINDKNKALIRVLLHDLLNPIGAIKSANE
                     LILEGDDESHAMSNLIQRAVENCLTVLDSVRKMYRIDEKQYTPNLTSVNLLDSIQFAL
                     TILTHKLSEKNIKINLDISNDIQVLVDEGPFINSVFNNLLTNAIKFSFPNSEISIKAI
                     CEKDFIVLSIKDTGIGMPEEVMNHLFDIGKSVSRKGTKGEQGTGYGMPLVKKFVEMFG
                     GKIQVDSIDKSKPNHGTEVKLFLKNAN"
     gene            27406..33096
                     /locus_tag="PLF66_07145"
     CDS             27406..33096
                     /locus_tag="PLF66_07145"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HMZ63852.1"
                     /translation="MLKNGIIGKNAGMISLDRTDTIDEIYTSDKTTIFRSTQNNTSII
                     IKTLHKEYPEPEELSEFKNEYEILKKLNGKGLVRALGFEKFNSNLAILFEDIGGTDLS
                     TLLYQEAFSLQDCLDIMIKATKALAHVHNANIVHRDIKTQNIVLNRKTGELQIIDFGS
                     ASFLLKQNSFIPMNSSLEGTLAYISPEQTGRMNRTVDYRTDYYSLGVTFYQLLTKELP
                     FVYSDPMELVHAHLAKVPVSPYTKCRIPKIISDITMKLLQKNPEDRYQSAMGIVYDLE
                     WSLKNIVEAYTIMPPQDRDSIIDRDDFLIAQNDFTTRFQIPEKLYGRSKEIIQILKSF
                     KNVTEGDVELVLISGRSGSGKSILINEINKPIVEYKGYFASGKYDQYKRNIPYYGITL
                     AFKNLLKQILTENQESIQYWKENLQDAVGANGKVIIDVIPELETLLGEQPPVVELGIS
                     ESQNRFNLVFQNFIKAFCMYKHPIALFIDDLQWADNPSIALIQSILTNPEVKFLLLIL
                     SYRDNEVLQTHPFFVMLEHLKEINISYKSIFLDSFSVKDVTHIVQDTLHCEKIYARQL
                     ATILHTKTKGNPYFVMEMFRSFYERDLIEYVGGKWVWNIIKIEEAKVSDNVIDLLVEK
                     VKDLTLSQVETLKLAACIGDSFSSEILYMVSKKTRDVLTEELTKISNDGFLIISKNTI
                     KFVHDKVREATYTLIGDAEKTKNHYLIGKAFIQLIKPDQIEDFIFVIVNQLNQSLGLL
                     ETDEERKDVIRLNTIAGNKSLAASAFDSALVFFNIAVSLLPEDAWQVDYKETIQLYSL
                     KARAESLSANYLMAESTFEAIATNAKGILDKINAYELKSSLYSSQLRLRDSIILAANA
                     LKLLGIKLPMKPTELSVIPELIKAKILIGHRSVSDFYNLLIMENESDLARMRLLSICI
                     PPSYLSLPLLFPVLVMKMVSLSLSKGISMLSPFAFSIYGMILGSALGDYKTGSEWGKL
                     AIDLIDKYDFKSVKAKTYMVYGCSIHHWTHHAKTNEEFILKAIQAGIENGDSEYASYA
                     LIHLEFQAFAMRKPLNEVIDLFNKMRPTFLKIRQEHAYDTASIIEQTSVNFVNLNSNY
                     KKLSGEIFNEEKSVPLWTAANNSSLLNCYYTVKCLIGYFFEDCEIVESYCKLAKKHEK
                     SNLGTMFLPEFTLFETLNLGKLYLNTESFYLRMQYLRRMLQNKKKMGTWAKNCEDNFG
                     HKFHIISGLIYFIKGKLNHAIVEYRKAIDLAKKSDYLLEEAIANELCGSVWKELNADD
                     YEKLHLTEAYYAYKKWNFLVKVKELEERYPFLKRRYAEPSSNVLVTNSKTVTGGNFLD
                     LNTVVKASQTISGEIQLGKLLGKMMKILFENAGAQRGYFILKDKDQLRIEAAGDSETD
                     SIVVLQNIPITGDEISIGIVNYVARTKTIVLLNDAPKSGIFINDTYVQEKNPKSILCY
                     PIINQGILVGLVYLENNLITDAFTPERVEILKVLSSQIAVSVENSLLYARLEEKVEER
                     TRDLNQALVEVNALKEQQDGDYFLNTLLIEPLAKNNAVSENIEIDFFIHQKKQFIFRK
                     EKYELGGDINISENIIIQDKKYIIFLNGDAMGKSIQGAGGVLVLGTVFKSIVERTMST
                     NYGKTVYPEKWLKDAFIEMHKTFVSFDGSMLMSIVFGLIDDRTGVMYFLNAEHPNIVL
                     YRDRQASFIDNGNQYTKLGTQGQTGSISVQVFSLLPQDMVIFGSDGRDDIIIGKEEET
                     GYDILNLDSELFLRHVENAEGDLKKIYENILSTGKPIDDISLGKIYFKNYDNSKSKVK
                     LETAISQLTYYKEQNDQNKLVELYLELISDYPHLTNYILEIAILYKEMNQIENALLFA
                     ERARIRQPKNLNNLLLLVQIYLGKGNMEKAKILLNTCKELNAEDPRVLELSSQL"
     gene            33652..33993
                     /locus_tag="PLF66_07150"
     CDS             33652..33993
                     /locus_tag="PLF66_07150"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HMZ63853.1"
                     /translation="MNYTHSVVNGFQILDLIGSIDLYSTPLIKKFAKTLLLSAETKVI
                     VSLEKVTFLDSSGLGMLANLFFECKQRSIPIVLANLSPAAKRILTLTKMTNSYQIFDS
                     IEEAIQSISNP"
     gene            34160..34495
                     /locus_tag="PLF66_07155"
     CDS             34160..34495
                     /locus_tag="PLF66_07155"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HMZ63854.1"
                     /translation="MPFSHKYISGVDVIDIEDSMDLYTVPEIKKFCRNLTFKENAKIL
                     LTLKKVNFMDSSGLGMLTNIFFECKQKAIPLKLADLSGEVRRMLSLTKLDLSFPIYET
                     QEEAIKSFD"
     gene            complement(34492..36345)
                     /locus_tag="PLF66_07160"
     CDS             complement(34492..36345)
                     /locus_tag="PLF66_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017862669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HMZ63855.1"
                     /translation="MIPAIVVSLCLIVYILGYRFYSRYLAEKVFVLRGSDFKTPAHIH
                     NDGVDYVPTKPSVLFGHHFASIAGLAPILGPAVAVIWGWFPAMVWVVLGGIFMGCVHD
                     MGALIISVRHDGKSIGQVAEDLLGHRARSLFHIIIFFLVSLAMGVFVLVIAGLYSAPP
                     KPIEIKTPTVIEQPVTVKDVKDGHDSGELKKESPKFHLRSNFPESVTPTLALMILALI
                     VGYLHYKKSFSLGWLTVVSFFITLVVIYYSMNDSFLDLIGLNDIERAPSTDSWKLILL
                     AYAFLASVTPVWLLLQSRDYINSFLLYLGIILVYLGYFVGSFSGNFPSFNASAFRTDG
                     IGMDILPFVFITIACGAISGFHSLVSSGTTAKQIAIEKDSRVIGYGGMIGESLLGLTA
                     VIACTIGFSSSEEWNSYYKSWAGLQGLNVQVGAYIYGTSRFLGEIGIPEKFGQGFIAL
                     IVISFALTSLDSATRLLRYNIEEIVHSFGSKTLNKILANRYSASLLACASIAFFAFLK
                     VNVNGQMKPAGLALWKVFGTTNQLLAGLALVVIFIYLVKTKRPMINIFLPMVFVLSVT
                     LWAMVGNFMEFLSGPNPNYLLAAVGGILIILTLWLLIEGFLVWRRIKGNQN"
     gene            37003..38355
                     /locus_tag="PLF66_07165"
     CDS             37003..38355
                     /locus_tag="PLF66_07165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63856.1"
                     /translation="MKTFKYIRILFFFFGLFIVCNQNHSPKITGIFNVTTTTNSNTIN
                     NNYNYSISVIVSGLSGSMTVSLNSAETLTINANGSYAFTTSLNSGTTYTVSIQSYSSG
                     QYCSGQNLNGVVGAANINISLTCQQGNSNGPLVGGTVFNPLNLSYTLTTIASGAMFNG
                     INGVTTDGTLIYFTVDGNHTILKLDPSTNTVNLIAGQTGVAGFTDGSALSATFNFPED
                     LVYLNNALYITDANNGAIRKMDLATNIVSTLATGVTLPYGITTDGTYLYITESGKVTK
                     ISISTGFVTVLAGSGVTGFADGVGATAQFSPSGTINGGIAFDGTDLFVNDRGNCAIRK
                     VIISSGAVSTIAGSPPPTAVCGTLLDGVGTASRINRADGIVSDGTNLYMADTNSCVIR
                     QIQLSNLNVTTVAGLAGSCSLVDGTGSAARLANPALLASDGVNIYISDWGNNAIRKMN
                     "
     gene            complement(38478..39356)
                     /locus_tag="PLF66_07170"
     CDS             complement(38478..39356)
                     /locus_tag="PLF66_07170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63857.1"
                     /translation="MSNRLHFYMNTSAFVTSIRIKIKENQYSTFLLIFAFCLFIASMT
                     LEKIIGNLLVERLEEDRKKEFTHGIQVSFEERWKIHTNQIRSYAYWPEVWDLIKAKKN
                     DKAYLNFQLDPSIDKQFDFFGIYLNDQKEFIIRWSEGSGEKRPPDFKTISHFYTTQSH
                     REGFTNYIIYFNQKYYLTTVTCLADNNGKPMIDGIMVFSYRFDKIISSITALFPIQIE
                     ILEGEDVPQQAYSSVSLDKLTLPGSKKLTLIFKPDISRAVLIRPYIISLLAILSVISF
                     SIYAWAIHKIRHADYD"
     gene            39494..41071
                     /locus_tag="PLF66_07175"
     CDS             39494..41071
                     /locus_tag="PLF66_07175"
                     /inference="COORDINATES: protein motif:HMM:NF024842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HMZ63858.1"
                     /translation="MNRKNFLYLMSAAISSSLLSSCKLFSGLPDNEFQGKFTGDNFSV
                     SHKHLWKIDEKEFAELKPQSEFDLIVVGGGLSGLSSAYLSDAEKVLVLDHASEFGGNA
                     KSQVWGDIRYSIGSAYITELSPSDPIYKFYISLGLDKHWKKIGEEDEIYFHSSGSFRK
                     LNAINDFQLRELPKVMKKYTGDLFPELPYSGNPKLTQSIFQSLDDKSLIDILRNEFRA
                     NLSSDLLSYLQTYARASFGSRAEDISGYAMLNFLSPEFETSIYCYPGGNSFMARTLVS
                     KLQERNCVLKNLNPVLNIKNVSDGVEVVSYDLKNQSYSKFKSKKVIVAAQKFVAKRIV
                     KDISIDQKNAMDALKYKTYFVANILLSKRPKENWYDSYLLTSADSWASDIVLADFPID
                     LDKRKQYSVLTVYMPYTSDTKRSEVLSKSASPELEKKYFAEMKSMVLSEVRLLKDKYG
                     FDEKDIHDINFTRWGHALVFSGKGMFSRNVFEKASSSIGNIYFANQDRIGTPSVESAF
                     TASFLALREAGLLRSGY"
     gene            41077..41616
                     /locus_tag="PLF66_07180"
     CDS             41077..41616
                     /locus_tag="PLF66_07180"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HMZ63859.1"
                     /translation="MTKAEARIYQKNRILKIENRQQKENLICERLTQRLIGAKRVIGY
                     RADQFEVNIDSVWKDKRLDSIDFYFPRVVSKEKRILEFVKPIGWETGAYGLEEPVGMD
                     LILPQEADLILVPSLGFHQNGFRLGRGAGFYDRAFREGSTKKMIGILFSELFSIDFPY
                     SDYDIRVGELVTESTVLFL"
     gene            41681..42133
                     /locus_tag="PLF66_07185"
     CDS             41681..42133
                     /locus_tag="PLF66_07185"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HMZ63860.1"
                     /translation="MEQTEIIQSEEVIQFLSFSVSNEIFGIELVHVHEILRPVFITRI
                     PNVEDHILGVVNLRGEIIPIVDLKKKFDQGFVEINKSTRIVVLENNSKRFGLVVEEVK
                     QVIKILKSTVSGINNHMSSSFNHMIDYVGRSGDTIVLIVELKKLINFE"
     gene            42148..45369
                     /locus_tag="PLF66_07190"
     CDS             42148..45369
                     /locus_tag="PLF66_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020766176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HMZ63861.1"
                     /translation="MSGILGEYTEIFLEESEDQIEELNANLLRLENDHNNPEIINDIF
                     RAAHSLKSSAAFVGLYNLSDLAHKMENLLQKIREGKFTINVNLVNLLFECFDLIKDVI
                     DAVARGEKKDNSYSEMIAKLENYEKSQEGQGGGQVISSAPKQPIQAKEEPTVAIAESA
                     PQSEASTSSSSAEPMIPLPLEEDEIRELEEELKLRNAKAYDVRVTLKKDTPMKGLRFT
                     LILQNLKLNAVIYKSSPDVESLERGIDQNFIVFILMTQMSSDDIYKLIMVDMVDSIDI
                     SLRNIQVAVSAPDKSEKIVQEARKERTAVQDKETGDKGALTKSIKVSSEKLDQLMNNV
                     GELVITNSGFQKIYDDLVRNFGDDTIFSELKSKIDQINRISKDLQSGIMNTRMVPIGS
                     VFNRFSRLVRDLSVETGKKVHLNLMGENTELDKKVVDMIGEPMLHLIRNSIDHGIESP
                     AERVKAGKPEYGTVELNAYQGGNNIMVEIKDDGRGLNKSRILKKAIENNLVSAADAQN
                     LADNEVYQFIFAAGFSTASEVTDISGRGVGMNVVNKLIQDFKGKILINTTEGFGTSFT
                     LCFPQALAIIPSILVSMEEEIYAFPLSEVFETIRIKKDQINTLEGHEIINLRGEVLPI
                     YRLNKIIGLADKLDLEEAPVVIVNYNSRKLGFIVDELIGKHETVIKTLEKNYKNIKGL
                     TGASIMGDGTIILVLDIGGLIDITTSTSLSTSHLVGKEMMRLNTSRSIDVNTSNVVFK
                     TQNSTNLFNNSLLGLQLVDKSKKKKDKTERKRVVSEQETVIRTAELNAEPIPVEPQKT
                     EPVAEAPKEELKKENQYLVVESEPESKPMEQKPVSNSVNIENLGDTSESDHEKARELL
                     KGFSDQTKKRVESLIHDRPINEMLSKEEIRKLESVVNTGMMNAGLVLSQLVGSNVELF
                     MPEIMLTDKDELVNEIRDANEHFFGMKVRMNGDLNGNLLMIFSEQRGKELAGKLLKEN
                     EESDLAKKMSDDSLSVLMEISNIVCSSVMNSLSNKAKAQIMPSVPELVTGNFKEVLDI
                     VKPEKTKFLSMNTEFIYEGDNLIGNLLFLPDFDELVKLISRLS"
     gene            45394..46461
                     /locus_tag="PLF66_07195"
     CDS             45394..46461
                     /locus_tag="PLF66_07195"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008595650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HMZ63862.1"
                     /translation="MTSLEFSRKIRAIVIDDSALVRNILTDLLQSDGMIEVIATGKTG
                     LECVQLAEKFQPDVITLDIEMPVMDGLTALENLSKKNIRVAVIMLSVLTQHGAKATFQ
                     ALELGAIDFVPKPSSAMKMDLQEIGSILKSKILGYFDHEIKRKAPRVKVKVGNFPEKK
                     NVPIRAVGIGTSTGGPNALHSLFKQIPADFQVPIFVVQHMPAGFTKAFAERLDEFSKI
                     KVKEAENGENVVSGTAYIAPGNYHMKVQRKDQNTSIELEQSPAVNGHRPSVDVTFDSL
                     RACYGNGLVGVIMTGMGKDGADSIKRVKEVGGFTLAQDEKTSVIYGMNRQAIEGGGID
                     QVLPLTEIVPNIIRIIKERGN"
     gene            46463..46825
                     /locus_tag="PLF66_07200"
     CDS             46463..46825
                     /locus_tag="PLF66_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002975323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HMZ63863.1"
                     /translation="MAKILVVDDAKFMRTLVKDALTAVGHTIVGEAENGNIAVEQYKL
                     LRPDLVTMDITMREKDGIVATGEIIKMDPKAKVIMVTALGQEDLLAKAIKMGVKDFVV
                     KPFPPERLQQAAAKALGL"
     gene            46837..47610
                     /locus_tag="PLF66_07205"
     CDS             46837..47610
                     /locus_tag="PLF66_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020771316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HMZ63864.1"
                     /translation="MEASDVQEENESLPFTIRWNNPDGGYSEGPLSLLWSLIESYKVD
                     IFDVSLSKITEDFIKFIQLSKNISIELSTDFIKMAANLVYLKSKSLLPNPGFEEIESD
                     AKLPKELVEKLLEHKKFQLAGKRLGEIDEITSGVFRRESNQILIDFPTDENWLDLDLI
                     DLISAFNSILQNKAVNEEVPNLLIANQDYSIDKKIQRIEELLQEKNEINFFDIFDTSE
                     PDTYDVVFSFLALLEIVKLKKVAIKQHKMFGNIKIFLVS"
     gene            47612..48199
                     /gene="scpB"
                     /locus_tag="PLF66_07210"
     CDS             47612..48199
                     /gene="scpB"
                     /locus_tag="PLF66_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017215788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HMZ63865.1"
                     /translation="MDNTQNTREYNKGLVEALIFLSGEPVKLGTLADSIKLEKSYVRD
                     LVEELILDYSERNGGFLLKEIAGGYQFLTNEAYYKVLGGIFKERKRETLSRGTLDTLA
                     IISYKQPITLPEIDEIRGVSSRNMVSTLIAKKLVKAVGQKEVPGRPTLYGTTNEFLIH
                     FGLNKLSDLPMPVEVKELKFEDLDDLKPEKEATDE"
     gene            48192..49271
                     /gene="pheA"
                     /locus_tag="PLF66_07215"
     CDS             48192..49271
                     /gene="pheA"
                     /locus_tag="PLF66_07215"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002632011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HMZ63866.1"
                     /translation="MSEDLSQLRQRIDELDIEIVNKIQERASVVSKIGEIKRSKGEEV
                     FRPDREKDVYKRVTRNNQGPLPDQTLISIYREIMSGSIAIEKALEVGYLGPEGSFSNQ
                     ATRERFGASIKSNSFASIPDVFRAVETGKCDYGVVPIENSTEGLVNSTLDMFLNSDLL
                     IYSEIYMRISLHLLGFETDLSKIKTLYGIRIANLQCKHWISANLPNVEIIETSSTAKA
                     AMMVAEKKEGAAIASKISADIYGLNVIRESIEDLSNNTTRFLIIGKSQCLPTGNDKTS
                     IVFSVADKPGALYLALKPFFENNINLTKVESRPTRRNSWEYNFFIDFHGHEKDEVISK
                     VLSELRDKATFVRILGSYPATEPLV"
     gene            49268..50164
                     /locus_tag="PLF66_07220"
     CDS             49268..50164
                     /locus_tag="PLF66_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016547773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HMZ63867.1"
                     /translation="MNSNFKKILIYGLGLMGASLALALKKKGYACKIVGIVRSEKSKQ
                     EGIALNIADEILIEEEFLKSNAWYHYDFIIFSLPVDLTCDKIKLIPSDYNGFITDLGS
                     TKKAIIETVEEKYTTNHNYYSSHPMAGSEQSGLNFAKPDLYEGRLCILTEPKNVNRDA
                     IEKITMFWREVGAIPLSLGADDHDEVLAYLSHTPHILSSLLVNWAYQNPKVKRNTDCS
                     PIAITGGGFRDMSRIAGSNPEMWNAIIQTNKDSIMNSLLEFRGEIDKMIQQLKSNDQN
                     DFWRDYFLQAKDSRNKILKIQE"
     gene            50214..50287
                     /locus_tag="PLF66_07225"
     tRNA            50214..50287
                     /locus_tag="PLF66_07225"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:50248..50250,aa:Ile,seq:cat)
     gene            51002..>51937
                     /locus_tag="PLF66_07230"
     CDS             51002..>51937
                     /locus_tag="PLF66_07230"
                     /inference="COORDINATES: protein motif:HMM:NF016196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 family protein"
                     /protein_id="HMZ63868.1"
                     /translation="MTEFFSKHADLIGVLFMPLTYGFVGWFTNWVALKMTFYPIKFWG
                     IPPYLGWQGIIPRKAHKMANRAVDVITGRLIKIEEIFDKVDPVMIEKELKPMLKPVIR
                     DLTKDIVDQIRPGLWPAIPDLVKNQIYAETEKQIPGAIKSIIMGIRKNVYQYFDLKGM
                     VLKRLSGDNVVLVVELFQTVGGPEFRFIELSGLYFGFLLGLVQIGIWLAFPVWWTLPI
                     QGVIVGYLTNWLALEMIFRPFYEKDYILFKYQGIFLKRQAAVAEQYSKFVATKILNAR
                     NILEEILYGRAADEVFALIKKAVIRSFENTSQLAHP"
BASE COUNT        17328 a         8198 c         9267 g        17144 t
ORIGIN      
        1 tattaccttg gtatgcaaaa atcaaatcta tttctcttaa aaagcgagag gaaattcttc
       61 catttagaaa tccaaatacg acacctactc aaggggtaag tcattattat ctggtcgagt
      121 tagaaactcc aatggatatg tctccgatca aagcaattga tgatttagaa attggcaaac
      181 caattgtgaa aaaatggaat gaaatattac aaatttaatt gtgaggaatt aataaaatga
      241 tgaaaactga aatacttgat tcaaatagaa ttatatacca attacgtgta aatgacattg
      301 cttcggtgat taaagaagat gagatggatt tggaattaac tgaagattta gtgttacatt
      361 tggaaaagag aatgagtaat tatttagatt ggagtgaagc cattcgcttt gctttagcgg
      421 attatcaaaa taaagaggaa ttatgaaata tctattattt gtaaattttg aaaacaactt
      481 aaaacacaat tctgtatttg aaacgataga ggaagtcata gaaagtgcaa agcatataat
      541 caaggatgaa gaattaaatg aggaattaga aaaatttcct gagacaaaag atttaattaa
      601 ccatttaaat accaatgatg attttttatt tacactttca aatgggactt ggtttcctat
      661 tcaatcttac cctgtttttt ctagacactt tcaactttaa aatactttct cccttcgtgt
      721 gcttcgtgct cttcgtggtt gaatctcttt tcaaaaaata gtaacttaaa aataactaac
      781 ccctgcggct aatatatttc aagggtaaga actatgaatc cgaatttttc tcatcacatt
      841 caatacaaac aaaatcaccc cagatatatt ttctattcat tgaacgacgt gtggaaatat
      901 cattcaaatc gggaattcga tttacaaatt taccggaacc aaaggcaaca gaatcaccgc
      961 attgattgca tcgttctcga tctttttctt ttttggtatt tttcataggt atcctctttg
     1021 gaaattcata attttcttat accgagcgtc aaaattaatt tcccattttt caatcacaaa
     1081 ttgatgtttg gtctatacca aattctattt aaataaagtc cttgtgggaa attacttttg
     1141 tcatttggta tatgagattt gaatctaaga ttaattataa agggagtaag gacaaagtag
     1201 gagagtgtgt tatttaattt tataaaaata aaaaaatttc atataacttt tgccataagg
     1261 gtttaaatgg ttttttttga gaaaattgat ttaaaacttt ttctctaaaa aactttgatt
     1321 ctaatggagt catttcttcg attctattac cagttaccct taacgcttca tcaatgtaat
     1381 tttttaagga attcattatc gtttatctcg aaaataagga acgcaatcta aaaatatatg
     1441 tcctttaatt ctctcatctc ttttaccact tgattcatag tatgcaataa aaacggaaat
     1501 tagttttatt tcaagaaaat attggtgatt tgaaatgcaa aaaaagaaaa tgcaaataaa
     1561 ttgagattta tttgtattgg tttgagaatt ataaaaagca atggagggga aaaatgaaat
     1621 taaccgttga ggatggattt ccttattata tagaattagt aggggagaag aattggaaag
     1681 aaatttacaa agcaaaagaa agtataaatc tatttgagaa aatagacgaa gaaaaagcaa
     1741 actatagatt tgaaagtggg aaatggagta ttaaacaaat tgtaggtcac ttgacagatc
     1801 atgagagaat tatgacttac cggatactgc gctttagccg caaggacgaa acactacttc
     1861 ctggatatga ccaagagact ttcattcaaa atagtaattt tgaaaaaatc ccatttgcgg
     1921 ttgtattaga ggattttaaa aacgtaagaa atgcatcgat tagttttatc aatactcttt
     1981 cagaggatca gtataaacta aaaggaaaag cgtggaagta tgaattgacc atagaggagt
     2041 ttttgaaagc aactatcggt catgaaatgc atcatttgaa tgtgttgaaa gaaaaatatt
     2101 taaggattac cacggatgca caccgatgaa cacagatgga taattattat tttcgagtct
     2161 tttatgaaag gttgcgattg gattcaagtg aattcgatat aagaattatt ttaacacagt
     2221 atgactttaa atttccagtg gtaagataat ttataaatat tttattgcat agaactgaca
     2281 tcgactacgc aattaaatta tcttttagat taacacctga aagttttttt gagtaggatt
     2341 ctgtaactag gtaaagaatt ttatctgagg ctaatttgta atcgagtccg ccaatacgaa
     2401 tgttggaaat gcaatttctt ttttcatcag tgaagccttt tttgggtttg taggtaagat
     2461 aagcgccaag gctatcaggg gaacttagac ccgggcgttc tccaattaag acgaggctaa
     2521 tgtctgtttg ctctaaggag gagatttcat ccccgatggc aacgcgacca tttttcacaa
     2581 gggaaaaaga aagagtaaat ccaagctctt ctaattttgg atttagaatt tttagcaaag
     2641 gaatgctgtt tttctcaatc gcgctactcg ataaaccatc cacgatacaa atcgaaatgg
     2701 attttgtttt tttctttgaa agattttttt tagaagaatc agaaagaatt cttcctttgt
     2761 cggggcgggt gagatattct tctctagact cacaggcgct ttccaattct tggaatttgt
     2821 atttttttct gagccagttt ttgatttcgg agaaattgat ttctgtgaga actgcgtctc
     2881 gtgcttttgc gtgcgccatt tgaaattgta acaattcttt cgtcggaatt ccaactccac
     2941 ttctaccgat tccgattctt gcggcggtgt gttgttttat ctttgtccaa ggatctaaat
     3001 tctttgtaat ttctttttgc ggcatagttg aaagattttg agtttaaagg gaaatgcaat
     3061 caattaaaaa tgaaatgaca gatttagaac cttaaaattt cataaaatta aattcaaaag
     3121 accaccgaag atagattcct ttttcttctt ttgtggtgaa aggatatctt atggttattt
     3181 caaaaaacaa agtagttgca atcgactaca cactgagaaa ttcagaaggg gaaatcattg
     3241 atgcttctgc tggacaagaa cccctcgttt acattcaagg aatcggaaat ctaattcctg
     3301 gtctcgaaga aatgttagaa ggaaaaaaag taggcgatac gttcaaagcc gttattcctc
     3361 cggaaaaagc ttacggcgaa tttgatgaaa gcctagtgca actcatccca agtaaacatt
     3421 ttgcgcaaat cccaaattta gaagtaggaa tgcaattaca agcaaacatg gatggacaaa
     3481 ttcgcattgt aactgtaact gcaatcgaag aaaattctgt tgagatcgat gcaaaccatc
     3541 cactcagcgg agagacgtta gactttgatg ttacagtaaa aagtatccgt gaagcaagcc
     3601 aagaagaatt agatcatggt catgtgcacg gagaaggtgg acaccatcat taataatcgg
     3661 caagtagttt tgccggagtc ttagatctag gaatcatttt ctcatctaag atttgcatac
     3721 gaatcagcca gtcttcaaac tctggagctg gctttaatcc taaaacctgc cttgcataca
     3781 atgcatcatg aaacgaagtg ctttggtaat ttagcatgat atcatcggct cccggaattc
     3841 ccattataaa agtgcaacct gctactccta gaagtgtaag caatacatcc atatcgtttt
     3901 gatccgcatc tgcatgattg gtatagcata tatcgcatcc catcggaagt cctaaaagct
     3961 ttccgcaaaa atgatcttcg agtccagctc gaatgatttg ttttccatca taaagatatt
     4021 ctggtccaat aaaaccaacg actgtattca ctagaagtgg gttgaatttt tttgcaactg
     4081 cataggctcg cacttcacag gtttgttgat cgagggaatg attggcgttc gctgataatg
     4141 cacttccttg ccctgtttca aaatacatta ggttttgtcc gacagttcct ctcttaagag
     4201 aaagtccagc ttcataggct tcctgtagta attttaaatt gattccgaag gatgtatttg
     4261 ttttttctgt tccgcctatc gactggaata ctagatcgat gggagctttt ttttgaatgg
     4321 cttcgattgt atttgtgaca tgcgtgagaa cgcaagactg ggttggtatt ttatattttt
     4381 ccctgacctc atctaacatt tttaaaagtt cgatcgcatt gtagacattg tcagtggcag
     4441 gattgattcc taaaactgca tctccacttc ctaagagaag tccatcgatt aaactggctg
     4501 caattccttt tagatcatcc gttggatgat tgggttgaag tctagttgat agtcttccag
     4561 aaagtccaat tgtatttcta aattttgtta ctacattgca tttttttgca acggaaatta
     4621 agtcttggtt tcgcataatc ttggatacgg ctgcaaccat ttcaggagtg agtccatctt
     4681 gtaaatcggt taacgtctca gtagttgtgt tttcagatag tagaaattct ctaaattctc
     4741 ccacactgag cccggatatt atttgaaaag ctttcttgtc gtggctatct aggattagcc
     4801 gcgtgacttc atctttttca tagggaatta attcttcctc taaaaagcgt tttaagggaa
     4861 cttcagagag tgtaatttgt gcggctactc tttcctcttg cgtgcttgct gaaactttcg
     4921 ctaatacatc gcctgatcta tgaggagttg ctttcgccat taatactatt aaatttttaa
     4981 actgataggt ttttttagaa acagttgttt tatacatgtt ttttatcctt tagataaaaa
     5041 taaaaatagg aatatgctag agttaaagta aataggtaaa tcattccaat ttcaaaatga
     5101 aatatcatca gagaaaaaag tgcaatgatt gcgaaaagaa acgcaatgaa aggagtgatt
     5161 ggaaaaaagg gaaccctaaa agaacttttg atatgagaat gatctttcct taaataaaac
     5221 agtgaaacca tagaaatgat gtagagtgta agtgctccaa agcaggcgag cgtgataatc
     5281 tccgctgttt ttcctgtaag gagtgccaat attcccaaaa ttgcatttcc tagaagtgcg
     5341 ttagctggcg tatgatacct tgcatttata tttccaaaaa ctttcggggc atatcccacc
     5401 cttgcaaatt cataggtggc tctcccagat gctaatagta ttccatgaaa ggaagcaatc
     5461 aaccctaaaa gcccaacaaa gactatgatt tgataataaa aagaattttt tccaattatg
     5521 gaagcaagcg ccaatggcaa aggtgaatca gacatttcct ttgttccact gatatataca
     5581 atactttccc aaccgttaat tcctatagaa gaaaaaaatg ttaatataca caaaaatacc
     5641 agtgtgagta atgcaaatcc aaatccgagt agtatattct tctgtggatt gattacttct
     5701 tctgctacat ttgcaatccc ctctattgca agaaaaaacc atattgcaaa tggaagagaa
     5761 gcaaagattc cagagattcc atttggaagt ggattcttat ttaaattgga aaattgaaaa
     5821 tgaggaagtg caatagcagc aaatagtaat aatgcgctca ctgctagtat ggttactatc
     5881 aattcaaaga atgctgccat tttaactgca aatatattta gagcggtaaa tacaaagtag
     5941 ctcccaattg ccgcaacaag tattggaatc gaaggaaaaa ataaattcaa gtaagcacct
     6001 attgccactg caatcgcagg tggtgcaaat acgaattcaa ttatctgtgc aattcctgct
     6061 atatacccga aatgatgatt caatgtaaag tttgcataat caaacgcacc gcccgccctc
     6121 ggtattgcac aagcaagctc cgtataacta aacgtaaacg ttatgtataa gactatgata
     6181 aatagtgttg ccattagaaa tccaaaactt ccacccctcg gcaatcctaa attccaaccg
     6241 aaatacattc ccgatataac gtatcctacc ccaagtcccc aaagcaaaaa tggtcctaaa
     6301 ttcttctcta atttactcat ctactttttc cttccctcca ttcacagaat cttatcaatt
     6361 taggaaagat ttttaatttt tagaaaaatc ttcgtggcct tatagaatta aggagccttt
     6421 ttacaactac cttagatttc gtcggaactc cgtcactcct tagtgatgca aatttttttt
     6481 tattaagctg gttttttttt tgaaaaatgc cgcacaaaac ttcaattttc tactcctatt
     6541 atagtaatag cactttgaaa tttataagag tttgtaaata aattgaattt aagaagatag
     6601 aagtatggga ttttaactta tttcagaaat ggatcaacat accctcttgc actcactttg
     6661 tatcgaaagg agtttttata tgagtgaaaa tttaaacgga aaaacatttt ctaaacaaaa
     6721 aatcttattt ccaagagaga atcttaaata tgcagagttt agctctacac ttttgattcc
     6781 cattgagtat ctagatttac ttcaaagtaa aattataaag cataaaggta tacgtagcta
     6841 tgtcgcctat cttctaggaa aatatcaact tcatattgca aatgggcttg ttcctggcta
     6901 ttcgaatgtc actacaaaat atcaggaaaa agatctgagt ttacaaaaaa tatcttttcg
     6961 ccctagacca gaagattggg cagaactaaa actttatcgt attagttttg gtatgtctat
     7021 ctccgccttt ttcgtatacc tactgattgc agattctgtt gattttgctg agaccgtttc
     7081 ccactaccta tccactgtcg gaattcctgt gatcgcaaat tttgacttga ccgcaaaagt
     7141 ctatctttgg aatcaaaaga cgttttatac gaaagttttc ttatatagga agggtaagta
     7201 ttattaatat aatactatat ttctaaaaat aaaacaattt atcttttaat ttcaccctgg
     7261 aaatacttga ttgttctttt tagaccttca atcaatggaa cgttgtactt atatcctaac
     7321 ttttctttcg ctaaagttaa atctggtctt cttctagctg gatcatcctg tgggagtgga
     7381 ttgtaaataa tttttgattt agattttgtt agttttataa ctagttcagc caattctttg
     7441 actgtaaatt cgtcggaatt cccaagattc acaggacctt tgaatgagtc ttggttcatc
     7501 atagagataa ttccatttac tagatcatct acatagcaaa aagaacgagt ttgactccca
     7561 tcaccataga tagtaatatc ttttccggaa agagcttgca ctataaaatt actcacaact
     7621 cgaccgtcat tcgcaagcat acgaggacca taagtgttga aaattcggat tacacggata
     7681 tcaacggcat gctgtctatt ataatcaaag catagagtct cagaaattct tttaccttca
     7741 tcatagcagc ttctaattcc aatagtattt acattacccc aataggattc tttttgagga
     7801 tgttcgagag gatttccgta aacttcactt gtggatgctt gtaagattct agccctagtc
     7861 ctttttgcaa gaccaagcat attaatagtt cccataacgt ttgttttaac tgttgagatg
     7921 gcattatatt gataatgaac tggggaagca gggcatgcga aattatagat ttgatctact
     7981 tcgattcgaa ggtcatttat gatatcatga cgaatcattt cgaaatttgg attttttaat
     8041 agatgtgcta tatttttttt tctaccggta aaataattat ctaccgatat aacttcattc
     8101 ccttctttta aaagtcgttc acataaatgt gatccaataa aaccagctcc gccagtgact
     8161 aatatgcgtt tactcatgct cattcaaccc ttcttttttt aattgtgttg cccagtcttt
     8221 atgaagtggt ttgccatcta ggtcaagccc tcttgatagg aaccattgtg caacatctgg
     8281 ttcaacattt gcttgtgcaa attgtttctt caatttactc atctttgcaa atgggtctgg
     8341 ttctgtaaaa gtttttttta caaaagttcc cagaagcaga acactaatac tagaccaaat
     8401 taaagcaatc ataactccaa gttgattcag atttagaccg agaaatcttt cttttttgcc
     8461 tatcatccag ttcgcaagaa ttcctgcatc cgattggtgg atatggtttg taaaatcagc
     8521 tagatcataa atactataaa gagaaacacc tgttccaaga aaaataagag ttagggagga
     8581 aacttctcga ctggttagtg agaggataac gaagattatc ccgaaacgaa ttccagtgtt
     8641 gtaagtaaag tcatgactcg atgagtaaaa atgagtaaat aaaattaaaa aaataccaac
     8701 aaagccgaga aaatgtttag cttgcttttt atgaaaacct ctatttagta gaattcctcc
     8761 taaaagagaa gaaccaatat aacctgctga gactacaaat agaaaagagg cttgtagttt
     8821 acctgaaatg acagtttctc ctgattcatt tccgtgaata aaaatcttct caacaacacc
     8881 gccggtaagt agagcagctg tcgcatggca aatttcatgg aataagacga taacagcttt
     8941 caggtggatc ataattttaa aatcccaaaa agctataaga gtgagaacga ttgaaaaaaa
     9001 gaatattaat ttttgtgatc tcacatcatt ctccaatact tacgaccgct ggctcaaatt
     9061 taaaataata ataaccacca gagttatctt caagttcaat atgatttgct tttgtcttct
     9121 taaaaaaaag tagggacttt actttataat taccctgacc agttgttata tctgggctta
     9181 atgttttttt attttcatcg acaaacatca tattgatttc gtttttatca gggtttttct
     9241 cttttattgt ttttatagaa tctgatagtt ttctatcaat attataacaa tctaaaactt
     9301 caacatgctt gtattctacc acttcattta atttaactct acacaattgg tgaattttgg
     9361 ggtttatttt tattttaatt ggttttgcag ttagataaaa aaatggactt ggattttttt
     9421 cttcaacaga aacaatagag gataatccta aatagccaca gaacaaaaat aaaagtataa
     9481 aattattcat tgaattaatt ttcccatttt tttataaaaa aaatcatctt catttcttag
     9541 aatcaattct tggatagatc tttttactga tttaaaatta tttttatttt tacagtatag
     9601 gtttaaaatt tcatcctgtt ccttttgagc ttttatcttg ccggatttaa gaatttcaca
     9661 ggattctttc tcgcctccac taataaaaag agttttggct acttctatca tttctgcgct
     9721 aataaatcca tcttctttct tagcatagct agcactcata tgaaaatacc gaatactttg
     9781 tctgtatttt tttttattcc ttaaatgcgt tccataaaag aaaaggatat ctgatttttg
     9841 ttcatttgtt aaagagctaa tcgacgcagc atctagaatt gctttctcta tacttgtagc
     9901 cgttgttgaa acgctagccg ccttagttat atacgctaat tttaaaacag gattaatatc
     9961 agggatttct gaaattagaa atgcaagaga atccaaatct ttagtttgaa atttaattgc
    10021 aaaaatataa tgaagcaaat aagaactagg gtagtagttg taaatactca gaagatcttt
    10081 tcttgaagtc tcatcatttt ttaaggaaat attaaccatt tccagaaaag ccgattcatc
    10141 tatttttaaa tttgtagaaa tattttttac gagttgatct gtttttttct gtcttacttt
    10201 gtctggagga aattttgaat ttaaaataaa tatctcttca aatctgctaa attttatata
    10261 taaaaaaaac aatctattga ttgtgttttt ttgaactttt tttgaaatat cggcttgttc
    10321 caaaactttt tcataaagat tgattgcaag taaaggagaa atcttttcga ttcgatatgc
    10381 ttcattgtat aagctttttg attcttttgc ataaatttgt tttgaaccca aagaagtcat
    10441 tatccctact aaaaggataa ataaaactgc tctcatcgta agacgaatta acttccaatt
    10501 tatttgcaag aatgattgaa ccaaaccctt taagtctttt ttcactttat ttctcatttc
    10561 tttttactac taacgaagtt ttggaacata gtcatgagat aggcgacatg ttccgttagg
    10621 ttttccttct tttgtgaata attccgaaac tctatctggg caaatatcag atgcataaag
    10681 acctgattct ctacatacaa cttgtcttgt tgcatgtttg ctgaatgcaa atttctcttc
    10741 tggaattaca ttcaaatctt taattttttt agcgattctt gcccagagcg gtgctgctaa
    10801 ttccgcaccc attccactct ttcccatgcc gtatgacgca tcatcataac ccatccagat
    10861 tgccattgat aatcttggtc tagttcccac aaaccaagca tcaatattat catttgtagt
    10921 tcctgttttt cctgctatct ctccgctata accatattta cgaattccgc tcgcatttgc
    10981 actgcctttc attaagctca ccataacttc ggctgtatct ggggcaatga cacgtctttc
    11041 ttctggaatc tttaattgaa attcatctct atcaaagtgt ttataaattt cctttccttc
    11101 ctgatttaaa atacgagtaa tcaaatacgg tctttttata attccgtcat ttgaaaaatt
    11161 tccatataca gctgccatct caattggtga gatttccagt gagcctagtg taacagaaag
    11221 atcattccta taacgtttac ttttttcatt agggtctgga aaaaagaagg aagaataata
    11281 tttttccaaa ttaggaagcc ccattctttc tccaatttga actgcaattg tgttttttga
    11341 ttttgctagg gcatctctta gattcatttc tccctcatag gctgaaccaa aattatcagg
    11401 tgtccaagtt ccttttccat ctggtgatga aaacataata ggagcatcta acaatatact
    11461 agccgcatta aaaattcctg aatctatagc ggatgcatat aaaattggtt tgatagaact
    11521 acccgtctga cgtctcattt gcactgcacg attgaattga tttttggagt taaattgctc
    11581 tccccctccg tgcataaata aaacagcacc agtcgaggga tctaccgcaa ctacagcagc
    11641 ctgtaattca tctgagacat ctgtgttgtc tggggtattt tttattttta ctcttttaat
    11701 tttccctttt tctttcatct ggttgaggtg aagtgctacc tcatcagaga gaacttcttg
    11761 aatttcttga atcaaagtag tttcgatcgt atatccaccg atatcataaa tattaatatt
    11821 tggatcgagt gattttagaa attctcgaat atgttctgta acgaatggag ccttatcata
    11881 cctacttcca aatacagtca tagaggctgg tgtttccaga gtttcataga acttgtttat
    11941 tttgttttga aaacgttcac tgattatatt tcttgcttcc agagtattta caattaccct
    12001 cactctgcct tcggacaatt ccctgttttt aaatggagaa taacggtttg gagcagacgc
    12061 aagagaggaa agtaaaatca tctccatagt ttttaattta tctggagttt tattaaaata
    12121 atatttcgct ccattttcaa ttccaaacgc cccatgacct agatacactt ggtttaaata
    12181 cgcttctaaa atttggtctt tcgaaagttt tgattctaaa gcgattgcaa ccattgcctc
    12241 tctgaatttt cttccaatgc tctttcttct gtcctttatt agaatacgag ccaattgctg
    12301 agtgattgta gaggcgcctt gaatataacc catattgatt atgtttttat aagtggctct
    12361 aagaagagca aagtaatcta tcccaccatg tttgtagaaa ttttggtcct ctactgataa
    12421 gataattttg attaacttcg gtgggtagtc tgagagtttt aaggttcccg tttttttctg
    12481 aaaaatttcg gataccaaaa caccatttct atctagtatc ttacttggtt tatgtcgatt
    12541 taattcgact agcaattcat ccacttctga ttttacttct atataactac tacgaattgg
    12601 aatgattata ctaattgata ttaagatcaa tagagcggct gctaaaaagt agcgatattt
    12661 tttagcgaac tgaagatatc gatttgaatc cgttttttca ggtaattgaa acttctgtaa
    12721 gaatttttca aaccaagtct cttgcttttt tattttaggg agagttactt tgtttatttc
    12781 caaaagaggt ttatttacag catccccaac agaaataaac tgagtatcct gtaaaaaaat
    12841 actttcgtat tttttatatt cttcttctgt taattcttta tttgtatcta cctttggggt
    12901 attttctgct tttgcctctg gttctagaat gaattcttga ttcgaattct cctcctccac
    12961 tctaacaaga gtctcatgct tacatttata acatgtaatt ttataggtcc ctggtctatt
    13021 tggtagagta gaaagtttag acttactatg gcaactctcg cagaaaaaac taccatcggg
    13081 cagtattcgc tcattatata gagttttcac gcatacatta tcggaacgat ttaaaaatcc
    13141 cgttagaata tactaaagta cttcgtaaaa ccattgatag agattactgt gaattagatt
    13201 caatcaactc actcaacacc aaaatggagt ttctcttatt gtgtcatcta aaacggaagt
    13261 ctttttctat taaattgatg agactaaagg atttttgaaa gtgtcaaact tttcaatcct
    13321 tgtgtattct tctacttctt tataagtccc agtgaccaga agcttctggc tggtaaataa
    13381 tttcatcgat ttgaaaaaac ttagtcccag tttccaaatc ccagcgaaca acatcccctt
    13441 tgcgggatcc aaagaccgca gttccgtatg ctgctaaaat ggaaattttg gattctgaat
    13501 caacttcttg cggataaaca agtgtgtatt cgtatctttg tcctgtgcct aaatctttca
    13561 gtctgaactt ggatcgcatt gtaattacat cgggaggtat tttatctgat ttaactgctt
    13621 gccttttttt tatttctttt tcaaaaactt ggctaagata tttatttgaa ttgaggctat
    13681 aaccctcttt taaaagattt aaaattcgtt ctttgtcaag ttgagtgatt aaaattttat
    13741 tcttaaacat acaaactcca agataattta ggcaagatga tatttgttct tattaaaact
    13801 caaaaaagaa tcactgaagt caagtttttt tctatttaat tttctaaact agaagtgtat
    13861 taaaatcaag cccttctgat tgattcatta gagtaactta atataggctc tcttttttca
    13921 tactgatccc ttttttattt tcatgatttt agttgttaac ttctagatat cgctctagtt
    13981 ctagaattca ggtttttaaa gcttgtctca atattaggtt ctagaaaata tatctaagag
    14041 gaaaatatgg agtttgttaa taaggttcgg ggtttataca tttccggaag ggcaattcgc
    14101 actctagttg agttgcttgg tgaatccaaa caagtcggtg taaaaataat gaagtttcat
    14161 aaaattaccg atgaccaagc ccctgaaaaa ttttcaatca atcccgatga gtggtatgat
    14221 ttagatcgtt tcctcttggg aatgcgtgat attcaggaaa gagtaggaga agaaaaaatt
    14281 cgtcaagttg cttacaacac gattcattat gctccactgc caactcacat ccaagatata
    14341 atgacatctc ttaatcacat gaataaagct tatttactaa atctctctcg ggatggaatt
    14401 caaactatgt tggatgagcc cggcggaaaa cagagtgaag cggttggaaa aatttcagta
    14461 actcccgtgc ctggaattcg aaaagtgatt tgtctaagca ccagttgttt tccaactttt
    14521 tatgagttag gactcatcga aggctttgcg aaaaaatttc agccaaaggc aacggtgact
    14581 ttagataaaa caagaattca aaaaaaacaa ggtggggatt caaatacttt tattgtgact
    14641 tggtgaacta acgtctattg ggaagaatca tttaacgtga attcgaagta agaggttttc
    14701 gcaaccgatg acaccgatag gctttttatt cctttttatc agtgtcttcg atttttataa
    14761 atttgacagg tttagcacat gtcggtgatt cgctttaaat gattcctttt atttaatctt
    14821 tgctgcttct tcttcaaata ctttagctct cgtataagct tctgcatctt cttgacctaa
    14881 atattttact ctgagttctc ttaatttttg ttctttgtct ttatctgaaa ttccattgtt
    14941 ggaagataga aattctcttt ctgctttttc gtaatctgca atttttttat catgtgcagc
    15001 aatttcttta tttgcagctg caattctctc tgccccttcc tttccaaagt attttacttg
    15061 taaagacgtt agttgttttt ctctctcttc tgggctaaga cctgctaagt ccttctcgcg
    15121 taggcttatt tcagtcttgt aatggtcaaa agagtcttca cgaccattga cagcatttag
    15181 ataggaaccg taggtgttct ttctgagttc ttcgtatttc tttactctct ctgaaccact
    15241 taagtttttt gtagagtcca tgaaattttt tgaagcttgc ataaacgcaa cttgtgattc
    15301 ttccattcca aaaaataaat cagctttttc ttgacctaca atttctcttc ttctattttt
    15361 taagatttca taacgttcct gaaaggtgat gtttgcagga aattccattt ttctggcttc
    15421 atcttcatat tgaaaatagg ctttaaatag ttctttcatt ttttctttat ctggactagc
    15481 aaactttgta tctataaatt tataaattgt ttcatcgcat tgaagtgctg tataatctgc
    15541 tgggcatttt cttctaagtg cccaaacttc ccaaacgaaa tttactctac ctgtttttag
    15601 gtcttcaatt agttctaaat atcccttttt ttctgttccc tgaaaaggag aaagtgcctc
    15661 ttcccaatgg ctcgttgtat cgccaaatgg tgaaatcgtg tcgttagtgg atcgatttac
    15721 tgtgtcattt gattcttcct gagaggaaga agttgatttt tttttatctt ttgctccaaa
    15781 tagaaggtat cctaaagtga gtgcaagcgc tgcagctaca atcccaatga taattttttt
    15841 attcatattt atatcctgaa aagtatttat gttagatttg gaagttttaa gtttttgttt
    15901 ttggaatctg aaatttcgaa tggaaaagag ttcaactgag agattgcctc tcagttgagt
    15961 tttgtatcgt cgcccgaatt cttctgagga ttataatcct gcttttgcag tttgagcgat
    16021 acctaagtac caacctgtta catcaaagaa gttttgtccg ctccattgaa aattagtagc
    16081 ttgcaagtga tcaatacctg tagcaaacca ttttgcagaa gcttctcctt tgtaatttcc
    16141 ccatttttgt gactcgagtg gaaccactcc atcgtttgca ctaccaagac cgtagaacaa
    16201 accacctgcc caagtaattg gataggttgg tgccattaat acgtgttgaa ttggatctgc
    16261 ccaagccatt ttatttccat aggaataata ttttaatcca gtaaaattag gtgttgcagc
    16321 attaaaagaa ttcactgtac taaccgcgag tgatttaccc attgcgataa catccattgg
    16381 ttttgctcca tagaaaagtt gagagaataa cccaagcgca gtatttgcga aaggttgtaa
    16441 ccaacttgga ataaccccaa gaacgatatc tgctaaagga gcccctttgt gaacagcatc
    16501 cacagtacta acagtagcaa ctcttttatt agcgctagtg ttaaaaccta aagctgaatt
    16561 tgatgccatg tagcgaacta ctagaccacc ttgtgagtgt ccaattaaat gcactttagt
    16621 acatccattt gcagccatcc aagggataag agcatctctt gttaattcag atcttttttg
    16681 aatactatta agcggagaac ttcctggtgt tgttactttt gctccttgtg aacggagata
    16741 agtatccata ccgccccagt attttacgag accacctgca agaccttgtg tatcatcaaa
    16801 tccaagaata ccatggacaa gtgcgatgca cattccgtca agtggaccag caaagagacc
    16861 tactgggagt gagaagaaga gggtgaagat gagtgttaaa aatttagctt tcatgttatt
    16921 ttcctttttc aatttatttg tatttgcata cacttaatgt ctataaatgc tttcaggcat
    16981 tgtcaagaaa aaattttttt tctgtatctt ttttcatttt tttgtaaaat aatttaagaa
    17041 aactagagag ccactcttat tttattagta attaagttat tgtggataat aattgttagc
    17101 cgttactctg tgcagaatgc aattcggtaa gtatttagaa aatatctaat tcgattttta
    17161 aaataaatct gattcttgtt atccgtgtaa cactgatttg ctgcaaaatc tttccttttt
    17221 cctccttaga ttgtatttcg tttggagtgt ttatttaata aatctatttc atttaagaaa
    17281 gggagatatt tatctcttta aactgattct tggggtaagg tattctcttg gtgtgcctgt
    17341 aaattctact gttaaaactt ccttgaataa tttaattttc attctgcatt gagtgattcg
    17401 tataactagg ttttgtcgct ctccctccca tttaaaagca gattcccttc aatttttctt
    17461 aaattttgta tataagatac cctacattcc atgagtgaaa taaaaatggt tgcggaaatt
    17521 cagtctatct cgtttgaata acctaaactt aaaaacaagg agattacttt gaaaaaattt
    17581 tatttgctaa ttttagctct catttttgtt aactgtgcaa agactgaata ttttactcga
    17641 aatgtaaata cacagattag aatgaattct ttttcagatg aagagggtta taaattaaaa
    17701 aaacgatttg agacttccca tacgcgaatc aatctttttt ggggtcttgt gactactatt
    17761 aacaagcgag atatggaatg gattttggct tccgaattaa aagatcctga taaagaagcg
    17821 attggaaatc taacaatcac cgaatactat gactttcttg atgggttagt ggatacagtt
    17881 ttgtttggac taataagacc ttactctgtt atcttaagag gcgatttata tgaaagagct
    17941 tctaatgctc caataattcc agaagataaa caaaactcag aaatgaaaga ggtaaaagat
    18001 gcgcccctcg aaacgtcaga tgcaaagtct gagaaagata ttgtaaaaga ttcagactct
    18061 gcgaaagact cctcaaaacc ttctaataca aaaaaaggag aaaaaaatgt taaaaagaag
    18121 taaatttttg cttattatcc taatgttatt gttctttatc aattgtgcaa gtcataccta
    18181 tcaaataaac aattacaaag ttcctgtatc ctttgcaaat gaagagggct cttctaaaaa
    18241 ctctagatac tttagaatcg aaaagaaatt aacttggctt atatttgatt tagtaaagat
    18301 acaagaattt gatttaacac atgtcttgga atcagaactt ccgaatgcta agaaaatata
    18361 caattttcgc attgaatcag aggaaaatgc tattgattcc gtgatccgta tctttggcac
    18421 cggaatgcaa tatttatttg cttccaatcg ttccattgtt tctagacgaa cggtgattat
    18481 ctctggaacc gtctctgaat aaaaattttt gaagtagtat ttctaatttt atccaatcta
    18541 aaaatatttt ttttgattgg atatttgttc ttatcaaaga gcaagctcat tctaaaaatc
    18601 ctatctgcct ttaataatac gtttagatca gtaaaatgct tttcaagttt ttttaaattt
    18661 caaatcttgt atcatgaaac aaatcggtta ttttttatct ttcctctttg cctattttct
    18721 atatttacct tttaaatttt taccatacaa agcttgcctt gcctatggag ttggaattac
    18781 caaagtccta tttccatttg caaaaaaaca cagaaagatt gcatacgata atatctctca
    18841 tgcttttcca gaatataccc atgaacaaag actcgcatta gttaaaaaac attttgaaca
    18901 tttaggtaga cttctcgctg gaacgttatt tgcctcacgt gtcaataaag aatggttaga
    18961 taaatattta gtctatgatc cagaatcact ccaaatcgaa gaagatacaa aaaaagaagg
    19021 agtgggcgtg gttctcgtct caggtcatct tggaacttgg gagatcttgg ttcaatttat
    19081 gggagttaga atgaaaggag ctggtatcta caaacagatt cgaaatccat ttgtagataa
    19141 atgggtgaaa tcactccgtg agaaaaatgg aatcgtcctt gtcacaacag aagaatcaaa
    19201 caaagtcatg aaacttttaa aagatggtta ttggattgga tttggctctg atcaaaatgc
    19261 aggcaaggct ggaatttttg tcaacttctt caatcgtcca gcctctactt atcaaggtcc
    19321 tgcacttatg gcttacctaa ctggcgcaaa attattatta tactctgtcg tctgtggtga
    19381 agatggaaaa gtgattgtgc gtgttaaaaa tatgggattt atcgataaga aaaaatttcc
    19441 aagccgagaa gcggctattc gccattatac ggaactttgg actcatcttt tagaagaaga
    19501 agtgaagcta tttcccgatc aatatttctg ggtgcataga agatggagaa caaagcccgg
    19561 agactttcct ggacaaacct aaatagtgtc ggttcgatgt aacagaaaaa ttaaaagatt
    19621 accaccgata gtgtctccga taaaagatac gataattcaa aaatatgtac cgtatcttca
    19681 tcgttcagtt atcggtgttc atcggtggtg aaaaaattgt tacatcgaat tcgctttaaa
    19741 tagcaaatgc atatccaatc caaaggttaa attgcaataa ccccgaagaa tttggatctt
    19801 tcgtttgtag atatttatca ataaaataga gattcgccgg cgtgtaataa ttattggaaa
    19861 taaaatttct ctcatctaca taggtataac tttttcgact gggatggatg ggaacgagtg
    19921 aacttgcttc taaatttaaa agtaaaccaa agtcaaatat ccattgcaat ccaactccat
    19981 actttgcata ccaatagggg ttgacggttg tagaattcga tattgcttct gtcgttagaa
    20041 cttggctagt tggatttctt gtaaataaaa gagtatttct agttgcccct ccatccaaat
    20101 attttccagc acctaacact agataaacag gtccttctgt gaagagaaaa aactttccat
    20161 ataaatcagc tcctcttcgg ttgtaaaact ctgcatgttg gtatgttgta taaccgacta
    20221 gtcggttttc ttttgtttct cttaaataat ctttgaaaaa atgagagcca attgtaaatc
    20281 tagaatttag attgtattct actcgtagct ctgcacttgc attgtaggaa aggctaacaa
    20341 cgagtttgcg ttcctctcta tttttacctt ggtttgaatc attttccttt tttgattcat
    20401 ctgtattaga gctagaagat tgcgaacttg tttcttcatt tgattttggt gtttcatcct
    20461 gagaaaaaat gaaattgtta aaaagaatta ggaaaagtag taaaacttta aaattcatat
    20521 ttatgcaccg gatgtaatct tgtcccaaaa atctcttttt ttgaaattat taaaatcact
    20581 ttgatgaaac gataggttgt atcgaataat taaagtattg agttgaaaga atatatcact
    20641 gaatcttttt ttgacaacat ctacattgca gttatgtgag taagtaatgt aagatctttg
    20701 attgactcga aagagtaaat ctcctttttt taaatgtctg agtatacctg ttttaatttc
    20761 gttatgaatc tcttcggtat attgtctgct catatgttgg tgatatgttt ttaagtcttg
    20821 tatttgaaaa tgtgatacga ctcctacttc ttcttttgat gtgaaacgac gacttatttc
    20881 tctaattacc ttatctaaaa attctgagta tgtatctatc acctgaaaat caattttctt
    20941 tggttgattc atataatagt ttttcacgaa ttcatttaga ttataaaaat tagattcgtt
    21001 aggagttttg tctgacggta tcaaaaagac agatcgaaaa atatttttat ttttttcaat
    21061 ggaataggta tagaaatcat acttttcatt ttctttttca atcgtcttga tcttactaaa
    21121 tcggatctgc ccatctaaaa gacttgctag taaatcaaat tttttaagat cctctgtcat
    21181 ttgtaataaa agtgaccttg gttctaattc agatccatat tctaaaagta aatcatcatt
    21241 taccgagaag gttgcttgca cacatggagt ctctttcgcg taagttttca gcattaactg
    21301 aaaatatgta tttgcgtcag gaagattaaa aattgtttcc gtcattctat aacccttggg
    21361 ttcagattcg agaatcgatt cgatacaagc aagcttattt ttgtattgat tctttgaaaa
    21421 attattttgc ggtaaatcag gttgaatttc gtaagcctct ttcgaaaaag gactgattta
    21481 taaaacttat atcatatctt actttttaga atggtctttt gtttatttta aaataaactt
    21541 ttcctattgg aagtccctcg acagttttca taaaggatca aaaggacaaa tgccaattta
    21601 atgaattagt tcttcgtttt tatttttatt taaaaagaga tagaaatggt ctgaatcaaa
    21661 tgaattatcc tctttgtaaa tcttttctaa aaacaaccct ttatcctatt tatcgatttt
    21721 atttaggata tatagatttg gtttcgaatt gagggcaatt ggattttgaa atgcaaagaa
    21781 agaaatatat ttcatggatt tgtttactgc aatcagacct agtaaaagtt ttcttaaaag
    21841 cgatagttgt tatagtagga taattatttc tagaaaatat ttagaatctt cgagaaaaaa
    21901 tctaatctaa aaactcttat ttagagaata tatgaaactt tccgagattc ttttattcaa
    21961 ataacactca agaatcagtg gaaaataata gaaccttact tttctaaaaa ggaacatgct
    22021 tactagattg cagattttta gtttgagata gtaaataaag aattgtcaaa gactgtatcg
    22081 gatggttttt tatatgtatg agatctgctg aatatccatc caacgagaaa gaaaggttaa
    22141 aaaaattaat tgattatagt attttagata cattagaaga gcaggaatat gatgagttga
    22201 ctcatttagc ttcttatata tgtaaaactc cgatcgcgtt aatcagtctc gttgataaag
    22261 ataggcaatg gtttaagtct agagttggtt tgaatgcgaa agaaactcac agagatcttg
    22321 ccttttgcgc ccatgccatt ttagacaagg aaatttttgt agttcccgat tcttctcaag
    22381 atgatagatt ttctgataat ccactttttt taaatgatcc tcatgttcga ttctatgctg
    22441 gtgctccact tttatctcca gaaggattgc ctgttggaac tctttgtgtc atcgatcata
    22501 ttcccagaga gctttcttca gaacaattag tcgccttaaa gactttaagt aaaaatgttg
    22561 tcaacctact tgaacttcgt ttaaaaatga aagaacaaga gaaacaaata gaacttttat
    22621 ctgaatcaga aaatcaactt tttcaagcat cttacaaata tgaatctttg ttaaatagta
    22681 gatcggtatt tattttaaga acggatttga gcggtaaata ctcatttgcc aatcagtatt
    22741 attctgatac atttcaaatg gatcctaatc atattttaga aatggatact ttttctcata
    22801 tattagaagg agatcatttc aaagtaaaag agacaattca aaaatgtata cattccccag
    22861 aaactccttt tcttgtttct ttgagacaat tgcaaccaga cgggaatatt ttatattctg
    22921 attgggagta tgtagcaatc aaagataaaa atggaaagtt agttgaaata caatgccttg
    22981 gaattgatat tacaaaacca agaaaagcat ttttggattt agaaaactct ctattaaaag
    23041 ttcacaatct agaatctgca atgaatgagc actcaatcgt tgctatcaca gattcagaag
    23101 ggaaaatttt atttgtgaat gaaaagttct gtgaaatttc aaaatttacc aagcaagatc
    23161 ttttaggtca aactcattct aaagtaaatt ctaaatttca tccaaaggaa ttttttgaag
    23221 agctttggaa tactatttta aatggaaagg tatggaaagg tgagattcga aatgtatcta
    23281 agaacggagg attttactgg gtcagttgca ctatcgttcc ctttttaaat tcagaaggaa
    23341 ttccctatca atatatttct ctccaaacag atataacttc ccagaaaagg gtagaagaag
    23401 atttaaagag aagtagagaa cgatatagta gttctcttga aaatatgttt catggttgtt
    23461 ttttaattgg atacgatatg agatatatct atgtgaatca aatgggagct atattaggta
    23521 gaaaattaaa ggaagaattt ataggtagaa caatgtccga tgtttacccc ggcatagaaa
    23581 atacctatat ctatcaatct gttctaaatt gtatgcgaga taaaaaaaga actcgatttg
    23641 aaacagaatt tcattttgaa gatggtggca ttggatattt tgaactaagt gtagaacctt
    23701 cttctgaagg agtttttata gtatctacag acatatccaa tcggaagata ttagaacaac
    23761 aaatcttgga tcaaaaagat tttattcata aagtaacgaa tacaattcct ggaatgattg
    23821 gatactggac gagtgactta cgttgtgcct ttgccaatga tgcttataaa gaatggttcg
    23881 gaatgagaga ggatattatt ggaaaatctt tagaggagct tcttggaaaa caaatttttc
    23941 aaaagaattt tgttcatata gaaaacgttc tgaaagggca aaagcaaatc tttgaaagag
    24001 aaccaacaga gagagcaggc tatacatgga ttcaatatat accggatatc gttgatcaaa
    24061 aagtaaaagg attccttgta ttaattacag atataactga tttaaaagta acggagatct
    24121 ctctgattga agcaagaaat aaattaagag agattttgga ggcaattcca gagggcttgg
    24181 ttgaaattaa tctccgcggt gaaatcatat atgcaaatca aggtgccgga aagattttgg
    24241 atattcatcg aaatggtgtt gagggtaagt attttaattc aagagcttgg caacaagttg
    24301 atcaagatgg aaatccattt cccttggaaa aattacctct atccattgcc atgaaagaag
    24361 agagagaagt gggaccgatt gagcatggag ttgtagattc taccggaaag aagaaatggt
    24421 tatccgttca tgcagttccc ctttttgata aagataagaa aatgtatggg gcagttgcaa
    24481 gttttcggga tataacagaa agacaaaagt ttcaagaaat gattattcga tcaaaagaag
    24541 aagcggaact tgcaagagaa atggcagata aagcaaacaa agcaaaaagt gagtttcttg
    24601 caaatatgag tcatgagatc agaacaccta tgaatgcaat ccttggattt ggtgagctgc
    24661 ttgcttctag aataatggat gaaaatttaa aagaatttgc agagtctatc actacaagtg
    24721 gaaaaatatt gctcaaactc ataaatgatg tgttagattt atctaagata gaagcaggta
    24781 aattagaact caattatact ccgatccata tcgataaaat tttatcggat atgaaaacaa
    24841 ttttctcgca aaaaattgag gagaaaaatt taaaactcat taccgagata gaatctaacg
    24901 ttccagaata tttaatttta gatgaaacaa ggctcagaca aatacttttg aacttaattg
    24961 gaaacgcagt caagtttaca gatactggtc tagttcgagt aattgtttcc acagagttta
    25021 aaacaaatca aaaacgaaaa gttgatttaa agattcaaat agaggataca ggtataggta
    25081 ttccctctaa agataaggaa aaaatctttg aagctttctc gcaaagtgat ggacaagatc
    25141 acaatcgtta tggcggcaca ggtcttggac tcacgattgc caaaaaaatg gttcagttga
    25201 tgaatggaga aatgtctatg gtgagcgaag cgggaaaggg aaccatgttt gttttgaaat
    25261 tcttaaatgt ttctatttcg tctatcggtg ttgaggatga ggatagaagc attgatgtaa
    25321 aaaatatatt tttttctaaa gcgaaaatat taattgctga cgatgtgaaa ttaaatcgtc
    25381 aactcctcat aaaattttta gaagatcagt cagagttgga aatcattgaa gctgagaatg
    25441 gcaagattgc tgttgaaaaa gctgtcaaac ataaacccga tattattttt atggatgtaa
    25501 aaatgccagt aatggatgga ttggaagcaa ttcaaattct aaaaaaagat cctgaaacaa
    25561 aattaatacc tattttggtt ataactgcat ccgtatttaa acaaacgaga gatgaagttg
    25621 ttgcgatctc tgatggattt ttgcaaaagc cagtgagtca aaaaaaatta atcagtaaaa
    25681 tgattgacta tcttgattat aagcgagtga ttcgggaaga aaaacaaaat gccgtagaat
    25741 ccaaaactca aattaccatt gatcctaaac tagctatgga aatgtatgat aagattatga
    25801 atgactctta tcataagtgg cttacattga aagatattat gaatattaca gagattgtta
    25861 aacttgcaaa tgaaatccaa aaaataggag aagagtataa atactctcct ctaatttttt
    25921 ggggaaagaa attggagaag tatgcaaatc tatttgactc taataaaatc aattcaacaa
    25981 tgcagcaatt ttctgattta ttaaaagttt taaagggtga tagatgagca atcaaacgat
    26041 tttagtggta gatgatacac ctgcaaatct ccaagtatta ggacaaattc tctctaaaga
    26101 aaaatataaa attatttttg cattcaatgg tgagcaggct cttaaatcat tagaacatca
    26161 aattccagac ttggtattac ttgatgtaaa tatgcctgag atggatggat ttgaaacttg
    26221 taatcgtatg aaacaattgg aattattaaa taatgttcca attctttttt taactgccag
    26281 aacagatatc gaagacattg ttaatgcatt taaaatagga ggggcagatt atattactaa
    26341 accttttaat cctactgaac ttttagctag aattcatttt catctttctt taaaagaaaa
    26401 gactgaacag ctaaaagaaa ttaatgataa gaataaagca ttaatacgag ttttacttca
    26461 tgatcttttg aatccgatag gcgcaattaa atctgcaaat gaattgatct tggaaggaga
    26521 tgatgaaagt catgcaatga gtaatttgat tcaaagagct gtcgaaaatt gtctcactgt
    26581 tttagatagt gtccgtaaaa tgtatcgaat tgacgaaaaa caatataccc caaatttaac
    26641 gtctgtcaat ttattggatt caattcaatt tgctttgact atacttactc ataaactttc
    26701 cgagaaaaac ataaaaatca atttggacat atcaaatgat attcaagttc tagtggatga
    26761 aggtccgttt attaattcag tgtttaacaa tcttctgaca aatgctatta aattttcttt
    26821 tcccaactca gagatttcca ttaaagcaat ctgtgaaaag gactttattg ttctctctat
    26881 aaaagatacg ggaattggaa tgcctgaaga agtaatgaat cacttgtttg atatcggaaa
    26941 atccgttagc cgaaaaggaa ctaaaggaga gcaaggaaca ggatacggta tgcctcttgt
    27001 taaaaagttt gtggaaatgt ttggaggcaa aattcaagtt gattctattg ataaatccaa
    27061 accaaatcac ggaacagaag tgaagctttt tttaaaaaat gcaaactaaa cttttacata
    27121 caatgaattt ctagatccac tgcagattct tttttgttag gagttcgaag ccttttttca
    27181 tagaagtagg aaaattccat tatctatacc tgtttgaaat tacttatatc gagcgagttt
    27241 ttttgataga attagagttg aatccttttt gcaagcatat cgaaaacgct ctttcaattt
    27301 acttttaagt tttatttaat tctaacttat tctatttaga atcaatataa aaaatcttat
    27361 atattctaag gtattttgta gtaaattata gacaaagaat cttccatgct aaagaatggg
    27421 ataataggaa aaaacgcagg tatgattagt ttagatcgca cagataccat tgacgaaatt
    27481 tatacaagcg ataagactac tatatttcgt tctacacaga ataatacttc tattataata
    27541 aaaactctcc ataaagaata tcccgagcct gaagagttaa gcgaatttaa aaatgaatat
    27601 gaaattttga agaagttaaa tggtaaaggc ttagttcgtg ctcttggatt tgaaaagttt
    27661 aattccaatc ttgccatcct ttttgaagat atcggcggaa ctgatttatc cactctcctt
    27721 tatcaagaag ccttttcctt acaagattgt ttggatataa tgataaaagc aacaaaagcg
    27781 ttagctcatg ttcacaatgc caatattgtt catagggata taaaaacaca gaatatcgta
    27841 ttaaatcgaa aaactggtga attacagatt attgattttg gttctgctag ttttctatta
    27901 aagcaaaatt cctttattcc aatgaatagt tctttagaag gaacacttgc ttacatttct
    27961 ccagagcaga caggtagaat gaatagaact gtcgattatc gcactgatta ctattctctt
    28021 ggtgtaacct tttatcaact tttgacaaaa gagttaccct ttgtctattc tgatcctatg
    28081 gaattagtcc atgctcatct tgctaaagtt ccagtttctc cttatacaaa atgtcgtatt
    28141 ccaaaaataa tttccgatat cacgatgaaa cttcttcaaa agaatccaga ggatcgatat
    28201 cagagcgcga tgggtatcgt ttacgattta gaatggtctc taaaaaatat tgttgaggct
    28261 tatactatta tgcccccaca agatagagac tcgataattg atcgagacga ttttttaatt
    28321 gcgcaaaatg attttactac cagatttcaa attccagaga agttatacgg tagatctaaa
    28381 gaaataattc aaatattaaa atcatttaag aatgtaactg aaggggatgt cgagctagtt
    28441 ttgatatcag gaaggtcggg ttcgggtaag tctattctca ttaatgaaat taataaacca
    28501 atcgtggaat acaaaggcta ttttgcttct ggaaaatatg atcaatacaa acggaatatc
    28561 ccttattatg gaattacact tgcatttaaa aatttattaa agcaaatcct aacagaaaac
    28621 caagagtcta ttcaatactg gaaagaaaat ttgcaagatg ctgttggtgc aaatggtaag
    28681 gtaatcattg atgtaatccc tgaactagag accctactgg gagaacagcc acctgtagtt
    28741 gaacttggga tatctgaatc ccaaaacaga tttaaccttg tatttcagaa ctttataaaa
    28801 gcattttgta tgtataaaca tccgattgct ttatttattg atgatctgca atgggcggac
    28861 aatccaagca ttgctttgat tcagagtatt ttaacaaatc cagaagttaa atttctactc
    28921 cttatcttat cctatagaga caatgaagtt ttgcaaactc atcctttttt tgtcatgtta
    28981 gaacatttaa aggaaataaa tataagttat aaatcaattt ttttagattc cttttctgtt
    29041 aaggacgtta cgcatatagt gcaagatact ttgcattgtg aaaaaatcta tgcaagacaa
    29101 ttggcaacta tcctacatac aaaaacaaaa ggaaatccct attttgtaat ggagatgttt
    29161 cgtagtttct atgaaaggga cttaatcgaa tacgttggtg gtaagtgggt ttggaatatc
    29221 ataaaaattg aagaagcaaa agtctctgat aatgtgattg atttacttgt tgaaaaggta
    29281 aaggacttaa cgctttcgca agtcgaaaca ttaaagttgg cagcctgtat cggggattca
    29341 tttagttctg aaattttgta tatggtaagc aagaaaacac gagatgtgct gactgaggaa
    29401 ttgacaaaaa tttcgaatga tggattctta attatatcaa agaatactat aaagtttgtt
    29461 cacgataaag taagagaagc aacctatact ctaatcggag atgcagaaaa gacaaagaat
    29521 cattatttga ttggcaaagc ttttatacaa cttatcaaac cagatcaaat tgaagatttt
    29581 atctttgtaa ttgtaaatca attaaaccag agtcttgggt tgttagaaac agatgaagaa
    29641 agaaaagacg ttattcgtct gaatacaatc gctggaaata aatccttggc tgcttctgct
    29701 tttgattctg ctttagtttt cttcaatatt gctgtttccc tgctgcctga agatgcttgg
    29761 caggtagatt ataaagaaac gatacaatta tactctttaa aggctagagc agaatcatta
    29821 tcagcaaatt atttaatggc tgaatcaact tttgaagcta tagctacaaa tgcaaaagga
    29881 attttagata aaataaatgc ttacgaatta aaatcttcat tatattctag tcaactaaga
    29941 ttgcgagatt caattatttt agcggctaac gctctaaaac ttcttggtat aaaattgcct
    30001 atgaagccta ctgagctttc cgttatacca gaattaatta aagcgaaaat attaattggg
    30061 catcgaagcg tatctgattt ttataattta cttataatgg aaaatgaatc agatttggct
    30121 agaatgcgtc ttttgagtat ttgtattcct ccttcttatt tatcacttcc attgttattt
    30181 ccggttttag taatgaagat ggtttctctc tctctctcga aaggcatatc aatgttatcc
    30241 ccatttgcat tctccatata cggaatgatt cttggatcag cactaggtga ttacaagact
    30301 ggatctgagt ggggtaagtt ggcaattgat cttatcgata aatatgattt taagtcagta
    30361 aaagccaaaa catatatggt ctatggatgt tctattcatc actggacaca tcatgcaaaa
    30421 acaaatgaag aatttattct aaaagcaatt caggcaggaa tagaaaatgg tgattctgaa
    30481 tatgcaagtt atgcattgat tcatttagag tttcaagcat ttgcaatgcg taaacctcta
    30541 aatgaagtga tcgatttatt taataaaatg cgaccaacgt ttcttaaaat aagacaagaa
    30601 catgcgtatg acactgcttc cattattgag caaacatcgg taaattttgt aaatttgaat
    30661 tctaactaca aaaaactctc gggtgaaatc tttaatgaag aaaagtctgt tccactttgg
    30721 acagctgcaa acaattccag tcttttaaat tgttattata ccgttaagtg tttaatcgga
    30781 tatttttttg aagactgtga aatcgtagag tcttattgca aattagcaaa gaagcatgag
    30841 aagagtaatc ttggaacgat gtttctaccc gagtttaccc tttttgaaac attaaattta
    30901 ggaaaattat atttaaacac agaaagtttt tatttgagga tgcaatattt gagacgtatg
    30961 cttcaaaata aaaagaagat gggaacttgg gctaaaaatt gtgaagataa ttttggtcat
    31021 aagtttcata ttatttctgg tctgatttat tttataaaag gaaaattaaa ccatgcgata
    31081 gttgaatacc gcaaagcaat tgatcttgct aaaaaatctg attatttact agaagaagcg
    31141 attgcaaatg aactttgtgg atctgtttgg aaagaactaa atgcagatga ttatgaaaaa
    31201 ttgcatctaa ctgaagctta ttatgcctat aaaaaatgga attttttggt caaggtaaaa
    31261 gagttagaag aaaggtatcc ttttttaaaa agacgatatg cagagcctag ttctaatgtt
    31321 ttagtgacaa atagtaaaac agtgactggt ggtaattttt tagatttaaa tactgtagta
    31381 aaagcttctc aaactatatc tggagaaatc caacttggaa aacttttagg aaaaatgatg
    31441 aaaatacttt ttgagaatgc gggcgctcaa agaggatatt ttattctaaa agataaagat
    31501 cagttgagga ttgaggctgc aggtgattcc gaaacggatt cgattgttgt tttacaaaat
    31561 attccaataa caggcgatga aatatcaatt gggattgtaa attatgtagc cagaacaaaa
    31621 acgatagttc tattaaatga tgctccaaaa tctggtattt ttataaatga tacttatgtt
    31681 caggagaaaa atccaaagtc tatcctatgc tatccaatta tcaatcaagg catattagtt
    31741 ggtcttgtat atttagaaaa taatctaata acagatgcat tcactccgga aagagttgag
    31801 atactaaaag ttctttcttc tcagattgct gtgagtgtag agaattctct actttatgct
    31861 cggctagaag agaaagtaga agaaagaact cgtgatttaa atcaagcgtt agttgaagta
    31921 aatgcattga aagaacagca agatggtgac tactttttaa atacactttt aattgagcca
    31981 ttagcaaaga ataatgcagt gagtgaaaac atcgaaatag actttttcat acatcagaaa
    32041 aaacaattca tatttagaaa ggaaaaatat gaattaggtg gagatataaa tatttctgag
    32101 aatataataa ttcaggataa aaagtatatc atattcctaa atggcgatgc aatgggaaaa
    32161 tcgattcaag gagctggtgg tgttttagtg cttggaacag tttttaaatc gatagttgag
    32221 agaactatgt ctaccaatta tggcaaaact gtttatcctg agaagtggtt aaaagatgct
    32281 tttattgaaa tgcataagac atttgtaagt tttgatggat ctatgcttat gtcgatagtc
    32341 ttcgggctta ttgatgatag aactggagtt atgtattttt taaatgccga gcatccgaat
    32401 attgttctgt atagagatag gcaagcatcc tttatagata atggaaatca gtatacaaaa
    32461 ttaggaacac aaggtcagac aggtagtatt tcagttcagg tattttctct tttacctcaa
    32521 gatatggtaa tctttggttc ggacggtaga gatgatataa ttataggaaa ggaagaggag
    32581 actggatatg atatattaaa tttagattct gaattatttc taagacatgt cgaaaatgca
    32641 gagggagatt taaagaaaat atatgaaaat attttatcta cgggaaaacc aattgatgat
    32701 atttctctcg gaaaaatata ttttaaaaat tatgacaatt ccaaatctaa agtaaaatta
    32761 gaaacagcta taagtcaact aacgtattat aaagagcaaa atgatcaaaa taaacttgtt
    32821 gaattatatc tagagttaat atccgattat cctcatctta caaattatat ccttgaaatc
    32881 gcaattttat ataaagaaat gaatcaaatt gaaaatgctt tactattcgc cgagcgtgct
    32941 cggattcgcc aaccaaagaa tctaaataat ttgctcttac tagttcaaat ctatcttgga
    33001 aaaggaaata tggagaaggc taaaatttta ctaaatacct gtaaggagtt gaatgcagag
    33061 gacccaagag ttttagaatt gagctcccaa ctttaatatt atctattttt atttgctaat
    33121 ctcatttttt aatacaagag atgtttcttg ctaatttcat ttgtaaaaat aattttgaga
    33181 aagaccgaag gaaagggtgg gagaataaac aaatacagtg tagccaaact ctcaacttgg
    33241 aattggtttc gttttatttt aaatttatct tttctaagtc aatgtagaat tggaatttta
    33301 aaaattaggt ttaaccgtta gtagaattaa tttcaggttc gctgactttt agtattagaa
    33361 agactgggat tttgagtgat aattttgttc ttttgaaaga aaactctcag aagtatgaat
    33421 tatgtatgtt ttaaaaaagg atttttattt gtatcaagat ataaatctat ttacagaatc
    33481 ataatttaag gaatagattt gtttacagaa ccaaagtaat atgaataggt ttatcattgt
    33541 tagtcggtgt atttctttgg tcaggttgaa atagaattga aaatacaatt atagtatatc
    33601 ttttggtata gaagattgag atatacaatg agtctataaa ggaagttaaa aatgaattat
    33661 acacatagcg tagtgaatgg gtttcagata ttagatttaa ttggatctat tgatttatat
    33721 tcaacccctc ttatcaagaa atttgcaaaa accttattgc tttcggcaga aactaaggtt
    33781 attgtttctt tggaaaaagt tacatttcta gactcatctg gactcggtat gcttgctaat
    33841 ctattttttg agtgtaagca aagaagtatc cctattgttc tcgcaaattt aagtcctgct
    33901 gccaaacgaa ttttgactct tacaaagatg acaaatagct atcaaatctt cgattctatc
    33961 gaggaagcaa tccaatctat ttcaaatcca tgagatatgt tttgaaattt ttattgcagt
    34021 cttttcctat ccattcaatc ttttactaaa ccaatccatt tcatagaatg gtttgtggat
    34081 ttttcagtaa aaagaaaaga tcgaaacgaa aactgagttt ttatattcta ttaaaatcaa
    34141 aactcgctgt gaggttttta tgccattttc tcataaatat atttctggag tagacgtcat
    34201 tgatatagaa gactccatgg atttgtatac agttccagaa attaaaaagt tttgcagaaa
    34261 tcttactttc aaagagaatg cgaaaatttt gctcactcta aaaaaagtaa actttatgga
    34321 ttcatctgga cttggtatgc ttacgaatat tttttttgag tgtaagcaaa aagcaattcc
    34381 tcttaaactg gctgatttaa gtggtgaagt tagaagaatg ctctctctta caaaactaga
    34441 tctcagtttt ccgatttatg aaactcagga agaagcgatt aagagttttg attaattttg
    34501 atttccctta attcttctcc agaccaaaaa gccttcgata agtaaccata acgttagaat
    34561 aattaatata ccgcctaccg ctgctaataa gtaatttgga tttggacctg ataaaaattc
    34621 cataaagttt ccaaccatcg cccatagagt cacagataaa acaaaaacca ttggtagaaa
    34681 aatatttatc ataggtctct ttgtttttac aagataaata aaaattacaa ctagagccaa
    34741 acctgctaat aattgatttg ttgttccaaa gactttccat aaagcaagac cagcaggttt
    34801 catttgacca tttacattta cttttaagaa tgcaaaaaaa gcgatagacg cacaagcaag
    34861 taagcttgca ctataacgat ttgccaatat tttatttaga gttttagatc caaaagaatg
    34921 aactatttct tcaatattat agcgtaaaag tctagtagca gagtcaagag atgtgagtgc
    34981 aaaactgatg acgataagcg caatgaatcc ttgtccgaat ttttctggaa ttccaatttc
    35041 acctaaaaag cggcttgttc cataaatata agcaccgact tgcacattca gaccttgtaa
    35101 tccagcccaa gatttataat aagagttcca ttcctctgag gatgagaaac cgatcgtgca
    35161 agcgatcacc gcggtcagcc caagaagcga ttccccgatc attcccccat aaccaatcac
    35221 tcttgagtct ttctcaattg caatttgctt tgctgttgtt ccggaactta ccagggaatg
    35281 gaagcctgaa attgctccac atgcgattgt aataaacacg aaaggtaaaa tatccattcc
    35341 tattccatct gttctaaacg cacttgcatt aaagcttgga aaatttccgg aaaagcttcc
    35401 tacgaaataa cctaaatata caagtataat tcctaaatac aatagaaatg aattaatata
    35461 atctctactt tgaagtaata gccaaacagg tgtaacagat gctaagaaag cgtaggctaa
    35521 taaaattagt ttccaactat ctgttgaagg tgctctttct atgtcattta agcctatcaa
    35581 gtccagaaag gaatcattca tactgtaata tattacaact aacgttataa aaaagctaac
    35641 aactgttaac caacctaaag aaaaagattt tttataatgt aaatatccga caataagcgc
    35701 aagtatcatt agagctaagg ttggagtgac tgattctggg aaattgcttc ttaaatgaaa
    35761 tttaggtgat tcttttttta gttctccgct atcatgccca tcttttacat ccttaacggt
    35821 tacaggttgt tcaatgacag ttggggtttt gatttcaatc ggttttggag gtgctgaata
    35881 gagaccagca atcacaagaa caaagactcc catagcaaga gaaactagga aaaaaatgat
    35941 aatatgaaat agagatcttg ccctgtggcc gagtaaatct tccgcaactt gtccaatgga
    36001 ttttccatca tgacgaaccg aaataatgag tgcacccata tcgtgaacac agcccataaa
    36061 aattccacca agaacaaccc ataccatagc aggaaaccaa ccccaaatga cagcaactgc
    36121 aggacctaag ataggagcaa gccccgcaat cgatgcaaaa tgatgcccga ataaaacgct
    36181 tggttttgtt ggcacataat caactccatc attgtgtatg tgagcgggtg ttttaaaatc
    36241 gcttcctcgt aatacaaata ctttctcagc taaatatctg gagtaaaatc tatatcctaa
    36301 tatatataca atcaaacaaa gtgaaacaac gatggcaggt atcatgtttt actaaggagt
    36361 tagaaatcag tcttgttgca agtagttttt aagatcgata ctcggttata tcaaatcctg
    36421 ttcaaatcag tgcttgtgga aaattactat cgcttttcta ccctctctaa tctattcgaa
    36481 taactagaaa tattaactaa atccaatcgg atcgttttcg tcttgatgat ctttttttta
    36541 cgagaaaata aaatctaccg aatgagagcc cattcctttt ttatatagag tttatactta
    36601 gttatgaaat gaaattatga atcagtttat gaaagtttat atctttaaat ctatttagtt
    36661 cgagtcttat ctctaaaaac catctagagt tttatcattt ctctccaatt ctacagaaag
    36721 tattttgttt tgtggtaaat taaaatatcg gattacatgg attagccatt tattttctgg
    36781 acaaaatttt ttatgatact aaaatgaagt cagtttttta atcgttctgg gttttattct
    36841 gaaaattttt taaattgcaa tgcaatcaga agaatgaaaa gacttagctt agtagaattc
    36901 atttcttaga tttatttgga aatgaaactt tattttggct ctattgtttt tattctcagc
    36961 atttttgaga atagagatat gaattgataa aaagtgaata caatgaagac ttttaaatac
    37021 atacgtatat tatttttctt ttttggatta tttattgtct gcaatcaaaa ccattctccc
    37081 aagattacag ggatttttaa tgtaacgact accacaaact ctaatacgat aaacaataat
    37141 tataattatt ccatttcagt tattgtttcg ggtttgtctg gaagtatgac ggtaagttta
    37201 aattcagctg agactttaac aatcaacgca aatggtagtt atgcttttac cacttctcta
    37261 aattctggga cgacttatac tgtttcgatt caatcctatt caagtggtca atactgttct
    37321 ggtcaaaatt taaacggagt tgttggcgct gcaaatataa atatttctct gacatgtcaa
    37381 cagggtaatt caaatggacc attagttgga ggaactgttt ttaatcctct caatctaagt
    37441 tatactttaa caactatcgc aagtggtgct atgtttaatg gtatcaatgg agtaacaacc
    37501 gatgggacgt taatttattt tactgtagat ggaaatcaca ctatattaaa attggatcca
    37561 agcacaaaca cagttaatct gattgctgga caaacagggg ttgctggttt tacagatggt
    37621 tctgctctat ctgctacatt taattttcca gaagacctag tttatttaaa taacgctttg
    37681 tatatcacag atgcaaacaa tggagcaatt cgaaaaatgg atttagcaac aaatattgta
    37741 tccactcttg ctacaggcgt tacattaccc tatggaatta caacagatgg aacttattta
    37801 tatataacag aatctggaaa agtaactaaa atttctattt ctactggatt tgttacggtt
    37861 cttgctggta gcggagtaac aggatttgcc gatggtgttg gagctactgc ccaattctct
    37921 cctagcggaa caatcaatgg cggtattgct tttgatggaa ctgatctctt tgtaaatgat
    37981 cgcggaaatt gtgcaatacg aaaagttatt atttcatctg gagctgtttc gactatcgca
    38041 ggatctcctc ccccaacggc tgtttgtgga actctcttgg atggagttgg cactgcttct
    38101 agaattaacc gcgcagatgg aattgtgagt gatggaacca atctgtatat ggcagataca
    38161 aatagctgcg tgattcgtca gatacaactt agtaatttaa atgtaactac agtggcagga
    38221 ttggcgggtt cttgttcttt ggtcgatgga actggaagtg cagctcgtct tgcaaacccg
    38281 gctcttttgg cttccgatgg agtaaatatt tatatctcag attggggaaa taatgctatt
    38341 cgcaagatga attgatcgaa tgcttcaggt tttaaagctt gatcgaataa cgcatcaaac
    38401 gattgaagaa tgatacttct gatttagagc tctgtgacag taagacaaaa ttttttttgt
    38461 cttatataga aattatttta atcgtaatct gcgtgtctta tcttatgtat agcccaagca
    38521 taaatagaaa agctaatgac cgaaagaatt gctagaaggg atataatata aggacgaatt
    38581 aagactgctc tagatatatc cggcttaaag atgagagtta attttttgga accaggaaga
    38641 gttaatttat ccagtgaaac agaagagtaa gcttgttgtg ggacatcttc cccttctagt
    38701 atttcaattt gaattggaaa taaagcagtg atagaagaga ttattttatc aaatctataa
    38761 gaaaaaacca taataccatc aatcataggt ttaccgttgt tatccgctag gcaagtaacg
    38821 gttgttagat agtatttctg attaaaataa ataatataat ttgtaaaacc ttccctgtga
    38881 ctttgggtcg tataaaaatg ggagatcgtt ttaaaatcag gaggtctttt ttcgcctgac
    38941 ccttcactcc atctgattat gaattctttt tggtcattta aataaattcc aaaaaaatca
    39001 aattgcttat cgatgctcgg atctagctga aagttaagat aggctttgtc atttttcttt
    39061 gctttgataa gatcccaaac ctctggccaa tatgcataac ttcgaatttg atttgtatga
    39121 atcttccatc tttcttcaaa ggagacctga attccatgag taaattcttt tttgcgatct
    39181 tcctctaggc gctctactaa tagatttcca ataattttct ctagcgtcat agatgctata
    39241 aacaaacaaa atgcgaatat taacaggaat gtggaatatt gattctcttt gattttgata
    39301 cgaatagatg taacaaaggc gcttgtgttc atataaaaat gtaacctatt actcaaaaac
    39361 tttattgtca ataaaactta gtaggagtgg cagaattttg acttttgaat cagtcaaatt
    39421 tccttagcag actagtatta agctgaccgt tggaagtgtc tgtataaatt ggtctctgtc
    39481 aagggataat tctatgaatc gtaaaaattt tttgtattta atgagtgctg ctatttcatc
    39541 ctctcttctt tctagttgta aacttttttc tggtttgcct gataatgaat ttcagggtaa
    39601 atttacaggc gataatttct ccgtctctca taaacattta tggaaaatag atgagaaaga
    39661 atttgctgag ctaaaaccgc agtctgaatt tgatttaatt gtagtaggtg ggggattatc
    39721 gggattatcc tcagcttatc tttccgatgc tgagaaagta ttggtattag atcatgcatc
    39781 agaatttggt ggcaatgcca agtcccaagt ttggggcgat atacgttatt cgattggatc
    39841 tgcctatatt actgaattgt ctccatccga tcctatttat aaattttata tttcactagg
    39901 gttagataaa cactggaaga aaattggaga ggaggatgaa atctattttc attctagcgg
    39961 atcttttcga aagttaaatg ccatcaatga ttttcaattg agagaacttc ctaaagtaat
    40021 gaagaaatat actggagatt tatttccgga attgccttat agcggaaatc ccaaattaac
    40081 tcaatcgatt tttcaatcct tagatgataa atctctcatc gatattctga gaaatgagtt
    40141 cagggcaaat ctatcctccg atttactgag ttatttacaa acatatgcaa gagcttcgtt
    40201 tggttcgaga gcagaagata tatccggtta tgcaatgctc aatttcttaa gtccagaatt
    40261 tgaaactagt atttactgtt atccgggggg aaatagtttt atggctcgca ctttagtttc
    40321 aaaattacag gaaagaaact gtgttttaaa aaatttaaac cctgttctaa atataaagaa
    40381 tgtttcggat ggcgtagaag ttgtttctta tgatttaaaa aatcaatcct attcaaagtt
    40441 taaatcaaaa aaggtaatcg ttgctgcaca aaaatttgta gcaaagcgaa ttgtgaaaga
    40501 tatatcaatt gaccaaaaaa atgcaatgga tgcacttaaa tacaaaacct atttcgttgc
    40561 gaacatatta ttatctaaaa gacctaagga aaattggtat gactcttatc tactaacgtc
    40621 agctgatagc tgggcgagtg atattgttct agctgacttt ccgattgatt tagataagcg
    40681 aaaacaatat tctgtgttaa cagtttacat gccgtatacc tcggatacaa agagatccga
    40741 agttttatct aaatccgcta gtcccgaatt agagaaaaaa tactttgctg aaatgaaatc
    40801 catggttctt tctgaagtga gactcttaaa ggataaatat ggatttgatg aaaaagatat
    40861 acatgatatt aatttcactc gttgggggca tgcacttgtg ttttcaggaa agggaatgtt
    40921 ttctagaaat gttttcgaaa aggcgtctag ctccattggc aacatttatt ttgcaaatca
    40981 agatcgaatt ggaactcctt ctgtagaatc agcctttaca gcatcgtttc tcgcattgag
    41041 ggaagcgggg cttttacgat ccggctatta aactctatga ccaaagcaga agctagaatt
    41101 tatcaaaaaa atagaatctt aaaaatagag aatcgtcaac aaaaagagaa tttgatttgt
    41161 gagcggttga cacagcgctt aatcggtgca aagcgcgtta ttggatatag agcagatcaa
    41221 tttgaggtga atatagattc ggtatggaaa gataaacgtt tagactccat tgatttttat
    41281 ttcccaagag ttgtctctaa ggaaaaaaga attctagaat tcgtaaaacc aattggatgg
    41341 gaaacaggag cctatggatt ggaggagcca gtaggtatgg atttaatact tcctcaggag
    41401 gcagacttaa ttctagttcc ttcgctgggg tttcaccaaa atggttttcg cttaggtaga
    41461 ggggcaggct tctacgatcg agcttttaga gaaggttcaa ctaaaaaaat gatcggaatt
    41521 ctgttttcag aacttttttc gattgatttt ccttatagtg attatgatat acgagtgggc
    41581 gagcttgtaa cagagtctac tgttttattt ttatagaata attcgataaa attgaccgat
    41641 aatctaacaa gcctaggatt tagaaggaat tgcggtttca atggaacaaa cagaaataat
    41701 ccaatcagaa gaagttatac aatttttatc tttttctgtt tcaaatgaaa tttttggaat
    41761 agagctagtt catgtgcatg agattttgcg tccggtcttt attactcgta ttcccaatgt
    41821 ggaagatcat attctaggag tcgtaaactt acgtggtgag atcataccaa tcgtcgatct
    41881 gaagaagaaa ttcgatcaag gatttgtaga aataaataaa agcactcgta ttgtcgtttt
    41941 agaaaataat tccaaaagat ttggtttggt cgttgaagag gtcaaacaag taatcaaaat
    42001 attaaagagc acagtcagcg gaattaacaa tcacatgtcg tcttctttta atcatatgat
    42061 agattatgtt ggtagaagtg gtgacacaat tgtattgatt gtggaattaa aaaaattaat
    42121 caactttgaa taatcaggag taataacttg tccggcattt taggtgaata cacagaaatt
    42181 tttcttgaag aatcggaaga ccagattgaa gagttaaatg caaatctcct acgactcgaa
    42241 aacgatcaca acaatcctga aatcatcaat gatatttttc gggcagcaca ttcattaaaa
    42301 agttcggctg cctttgttgg tttatacaac ctttctgatt tagctcacaa aatggaaaac
    42361 cttctccaaa aaattagaga aggtaaattt acaatcaatg taaatttagt gaatctatta
    42421 tttgagtgtt ttgatttaat taaagatgta attgatgcag ttgctcgcgg cgaaaagaaa
    42481 gacaattctt actccgaaat gattgcaaaa cttgagaatt atgaaaaatc ccaagaaggt
    42541 cagggtggtg gacaggttat atcctctgct ccaaagcaac ctatacaagc aaaagaggaa
    42601 ccgacagtcg ctatagctga atctgcacct cagtcagaag catctactag ttcgagtagt
    42661 gctgagccta tgattccact tcctttagag gaagatgaaa ttagagaatt agaagaagaa
    42721 ttaaaactca gaaatgcaaa agcctatgat gtgagagtta ctttaaaaaa agacactcct
    42781 atgaagggtt tacgttttac tctcattctt caaaatttaa aattaaatgc agtcatttat
    42841 aaatcttctc ctgatgtaga gtctcttgaa agagggatcg atcagaattt tattgttttt
    42901 atattgatga ctcaaatgtc aagtgatgat atctacaaac taatcatggt agacatggtg
    42961 gatagcatcg acatatcgct tcgaaatatt caggtagcag tatctgctcc agataaatca
    43021 gaaaaaatcg tgcaggaagc gagaaaagaa agaactgccg ttcaagataa agaaactggt
    43081 gataaaggtg ctttaacaaa aagtataaaa gtatcttctg aaaagctaga tcaactcatg
    43141 aataatgtgg gtgagttggt gattacaaat tcaggatttc aaaaaatcta cgatgattta
    43201 gtaagaaatt ttggggatga tacaattttt tctgaattaa aaagtaaaat agatcaaatc
    43261 aatcgcattt cgaaagatct gcaatctggt atcatgaata ctagaatggt tcctatcggt
    43321 tctgttttta atcgtttttc tagattggtt cgtgatttat cagttgagac tggtaagaaa
    43381 gtccatctca atctaatggg cgaaaacact gagctggata agaaagttgt ggatatgatt
    43441 ggagaaccaa tgcttcactt gattcgaaat tcgattgacc atggaattga atctcctgct
    43501 gaaagagtga aagctggtaa gcctgaatat ggaactgtag agcttaacgc atatcaaggt
    43561 ggaaataata tcatggttga gatcaaggat gatggacgtg gtctcaataa aagtcgtatc
    43621 ctgaaaaaag ccatagaaaa taatttagta tcagcagccg acgctcaaaa tttagctgat
    43681 aacgaagttt accaatttat atttgcagct ggattttcaa ccgcatctga agtaactgat
    43741 atctctgggc gcggagttgg aatgaatgtg gtaaataaac tcattcagga ttttaaggga
    43801 aaaattttaa ttaacacaac tgagggtttt ggaacatctt ttactctctg tttcccgcaa
    43861 gctcttgcta ttattccttc aatacttgta tccatggaag aagaaatcta tgcatttcca
    43921 ttgtctgaag tttttgaaac gattcgtatt aagaaagatc aaatcaatac tttagaagga
    43981 catgagatta taaatctcag aggcgaagta ttgccgattt atcgtttgaa taaaatcatt
    44041 ggtcttgctg ataaattaga tttagaagaa gctcctgttg taattgtaaa ttacaattcc
    44101 agaaaattag gttttattgt agatgagtta attggtaaac atgaaacagt tatcaaaaca
    44161 ttagaaaaga attataaaaa tatcaaagga ctaacaggtg cctcaatcat gggagatgga
    44221 accattattt tggtcctaga tattggtgga ttaattgata taacaacttc tacaagctta
    44281 tccacttctc atttagtcgg taaagaaatg atgcgtttga atacaagtag atcaatagat
    44341 gtgaatacgt ctaacgttgt atttaaaact cagaactcaa ccaatttatt taataatagt
    44401 cttttaggat tacagttggt tgataaatct aaaaagaaga aagataaaac cgaacgtaaa
    44461 agagtagtat cagaacaaga gactgttatt cgaacggctg aactaaatgc tgagcctatt
    44521 cctgttgaac cacaaaagac tgagcctgtt gcagaagcac cgaaagaaga attgaaaaaa
    44581 gaaaatcaat acctagttgt cgaatcagaa ccagagagta aaccaatgga acaaaagcca
    44641 gtatcgaata gtgtaaacat tgagaattta ggcgacacat ctgagtcaga tcatgaaaag
    44701 gccagagagt tattgaaggg attttctgat caaacaaaga aacgggtaga atcattgatt
    44761 cacgatagac ctatcaatga aatgctcagt aaagaagaaa ttcgtaaatt agaatcagtt
    44821 gtaaatacag gtatgatgaa tgccggttta gttttatctc agttggtagg atcgaacgtt
    44881 gagttgttta tgccagaaat aatgcttacc gataaggatg aactcgtaaa cgaaattcgt
    44941 gatgcaaatg aacatttctt tggaatgaaa gtaagaatga atggagattt gaacggtaac
    45001 ttactcatga tattttcaga acaaagagga aaggaattag cgggaaaact tctgaaagaa
    45061 aatgaagaat ccgatttagc taaaaaaatg tcagatgatt ctctctctgt attaatggaa
    45121 atatcaaata ttgtatgttc tagtgtaatg aattctcttt ccaataaagc aaaagcacag
    45181 attatgcctt ctgttccaga gttggttact ggtaatttca aagaagtatt agatattgtg
    45241 aagccagaga agactaagtt cttaagtatg aatactgagt ttatttatga gggtgataat
    45301 ctgattggaa acttattgtt tctcccagat ttcgatgagt tagtaaaatt aatttctaga
    45361 ttgtcatagt ttggttttaa aaaggaagta aagttgacca gtttagaatt tagcagaaag
    45421 attagggcaa ttgtgattga tgattctgct ctggttagaa atatattgac tgatttactt
    45481 caatcagatg gaatgattga ggtaattgca actggtaaaa caggtcttga gtgtgttcaa
    45541 ttagcagaga agtttcagcc cgatgtgatt acacttgata tagaaatgcc tgttatggat
    45601 ggtttaactg ctcttgagaa tttaagtaaa aaaaatatta gagttgcagt tataatgctt
    45661 tcagttttaa ctcaacatgg agcgaaagca acctttcaag cattggagtt aggagcaatt
    45721 gattttgtcc ctaaaccatc tagtgcaatg aaaatggact tacaagaaat tggatcgatt
    45781 cttaagtcta aaatacttgg ttattttgat catgaaatca agcgaaaagc accaagggta
    45841 aaagtaaaag taggaaactt tcccgaaaaa aagaatgtgc caattcgtgc agttgggatc
    45901 ggaacttcta ctggcggacc aaatgcttta catagtttgt ttaagcaaat accggcagat
    45961 tttcaagttc caatctttgt tgtgcagcat atgccagcgg gttttactaa ggcatttgcc
    46021 gaaaggttgg atgagtttag taagatcaaa gtgaaagaag cggaaaatgg tgaaaatgtt
    46081 gtctctggca ctgcttatat agcacctgga aactaccaca tgaaggttca aaggaaggac
    46141 caaaatactt cgattgagct agagcaaagc ccggcagtaa atgggcatag accctctgtt
    46201 gatgtaacat ttgacagttt aagggcttgc tatggtaatg gtttagttgg ggttatcatg
    46261 accggtatgg gcaaggacgg tgcggactca attaaaagag tcaaagaagt agggggattt
    46321 acattggcac aagatgaaaa gacgtcagta atctatggga tgaacagaca ggcaattgag
    46381 ggtggtggaa ttgatcaagt tcttccatta acagaaattg ttcctaatat tatacgtatt
    46441 attaaagaaa gaggaaatta aaatggccaa gattttggtt gtggatgacg ctaaatttat
    46501 gcgaacctta gtaaaggatg ctttgactgc agtcggtcat accattgttg gagaagcgga
    46561 aaatgggaat attgcagttg agcagtataa gctgttacgt cctgatttag ttactatgga
    46621 tattacaatg agggaaaaag atggaatcgt tgcaacaggt gagattatta aaatggatcc
    46681 taaagcaaag gttatcatgg taactgcttt agggcaagaa gacctacttg caaaagcgat
    46741 caaaatggga gttaaggatt ttgttgtaaa accattccct cctgaaaggc ttcaacaagc
    46801 agcagcaaaa gcactaggac tgtaattatt gactcaatgg aagcttccga tgtacaagaa
    46861 gaaaatgaat ctcttccatt tacgatccgt tggaataatc ctgatggggg ttattccgaa
    46921 ggtcctctct cgctcctttg gagcttgata gaaagttata aagttgatat ctttgatgta
    46981 tccctatcaa agataacaga agattttatc aagttcattc agttatctaa aaatatatct
    47041 atagaactaa gcactgactt tatcaaaatg gctgccaatt tggtttattt gaaatcaaag
    47101 tctttacttc ccaatccagg ttttgaagaa attgaatcag atgcgaagtt gccaaaggaa
    47161 ctcgtagaaa aacttttaga acacaaaaaa tttcaattgg ctggtaaaag gttaggcgaa
    47221 atagatgaaa ttacttctgg agtattcaga agagagtcaa accagattct aattgatttc
    47281 ccaaccgatg agaattggct agatttagat ttaattgacc taatatctgc atttaattca
    47341 atattacaaa ataaagctgt caatgaagaa gttcccaatt tactcatagc aaatcaagat
    47401 tattccattg ataaaaaaat tcaaagaata gaagaactat tacaggaaaa aaatgaaatt
    47461 aatttttttg atatattcga tacgagtgag cctgatacat atgatgtagt tttttctttt
    47521 ctagcgttat tggaaatagt taagttgaaa aaagtggcaa ttaaacagca taaaatgttt
    47581 ggtaatatca aaattttttt ggtaagttga tttggataat actcaaaata caagagaata
    47641 caataaaggt ttagtagaag cattgatatt tctatctggg gaacctgtca aattagggac
    47701 tctggcagat tctattaagc tagagaaaag ctatgtcagg gatcttgtag aagaattgat
    47761 tttagattat tccgaacgaa atggtggttt tttgctaaaa gaaattgcag gcgggtatca
    47821 gtttttaaca aacgaagcct attataaagt tctaggtgga atctttaaag aaagaaaaag
    47881 agagaccctt tctagaggaa cgttagatac tcttgctatt atttcttaca aacaacctat
    47941 tacactccct gaaattgatg agatacgcgg cgtatcttca agaaatatgg tttctacttt
    48001 aattgcaaaa aagttagtaa aggcggttgg tcaaaaagaa gttccaggca gacctacact
    48061 ttatggaacc actaatgaat ttttaataca tttcggatta aataaacttt ctgacttacc
    48121 tatgcctgtt gaagtgaaag aattaaaatt tgaagattta gatgatttaa agccagaaaa
    48181 ggaagcaaca gatgagtgaa gatttatctc agttacgtca gaggattgat gaacttgata
    48241 tcgaaattgt aaataaaatt caagagagag cttctgtagt aagtaaaatc ggagaaatca
    48301 aaagaagtaa gggagaggaa gtttttcgtc ctgatagaga aaaggatgtt tataaaagag
    48361 ttactagaaa caaccaagga cctcttccag atcaaaccct aatctccata tatagagaaa
    48421 tcatgtcagg ttctattgca atcgaaaaag cattggaagt tggatatcta ggaccagagg
    48481 gctctttctc gaatcaagcg acacgcgaaa gatttggagc atctattaaa tctaactcct
    48541 ttgcatctat tcctgatgta tttagagccg ttgagactgg aaaatgtgat tacggtgttg
    48601 ttccaataga aaattctact gaaggtttgg taaattccac tctagacatg ttcctaaatt
    48661 cagatttact catatactca gaaatataca tgaggatttc tttgcatctc ttgggatttg
    48721 agaccgattt atcaaaaata aaaacgttat acggtattag aatagcaaat ttacaatgca
    48781 agcattggat tagcgcaaat cttccaaatg tagaaatcat tgaaacatct tcgaccgcga
    48841 aagcagcgat gatggtggct gaaaaaaaag agggagcggc aattgcttct aaaatttcag
    48901 cagacatata tggtttaaat gtgattcggg agtcaataga agatctatct aataacacga
    48961 ctcgtttttt gatcattggc aagtcacaat gtttaccaac tggaaatgat aaaacatcta
    49021 ttgtattttc tgtcgctgat aaaccaggtg ctctctattt agcacttaag cccttctttg
    49081 aaaataatat caatttaacg aaagtagagt caagacctac tagaagaaat tcctgggagt
    49141 ataatttctt tatcgatttt catggacatg aaaaagatga agtgattagt aaagtcttat
    49201 ctgagttaag agataaagca accttcgttc gaatccttgg ctcttatcct gcaacagagc
    49261 ctttagtttg aactccaatt ttaaaaaaat tttaatttat ggtcttgggc ttatgggtgc
    49321 ttccctagct ctcgctttaa agaaaaaagg atatgcttgt aaaattgttg gaatcgtaag
    49381 atcagagaaa agcaagcaag aaggtattgc tctcaatatt gcagatgaga ttttaattga
    49441 ggaggaattt ttaaaatcaa atgcttggta tcattatgat tttattattt tctctcttcc
    49501 cgtcgatttg acttgtgaca agattaaatt aatcccatcc gattacaatg gtttcataac
    49561 agatttaggt tcaaccaaaa aggcaattat cgagactgta gaagagaaat atacaacaaa
    49621 tcacaattat tactcatctc atccaatggc aggttctgaa cagtcaggtt taaatttcgc
    49681 caaacctgat ttatatgaag gtagactttg tatattaaca gaacctaaaa atgtaaatag
    49741 agatgcaatt gagaaaatca caatgttttg gagagaagta ggtgcgattc cactcagctt
    49801 gggagctgat gatcatgatg aggtcctcgc atatttatct catacacctc atattctatc
    49861 ctccctgttg gtgaattggg cttatcaaaa tcctaaagtt aaaaggaata cagattgttc
    49921 tcctattgcg atcacgggtg gtggttttcg agatatgtca cggatcgctg gctcgaatcc
    49981 agagatgtgg aatgcaatca ttcaaaccaa taaggattcg attatgaatt cattattaga
    50041 gttcagagga gagatcgata aaatgatcca gcaactgaag tcaaacgatc aaaatgattt
    50101 ctggagagat tatttccttc aagcaaaaga ctctaggaac aagatattaa agatacaaga
    50161 ataaaaaaac ttgcgaatcc cattctttat aaaaacatga caaaggtatt caggggccta
    50221 tagctcagtc ggttagagcg gcagactcat aatctgcggg tcgaaggttc gagtccttct
    50281 gggcccaatc acatccatcc ttttttataa tcaaatctat tctgaaagta aggactcaaa
    50341 tagagtcttg tgttttttga aatcttggaa taccccatat ttttaaaata cgaaacagga
    50401 tgttgggcga acacgttcag ggggcaacgg ataatgcttt caacttaagg attttcaacc
    50461 acatagcttg gctatggtga gcgtcaagcg atggggggaa tacacgaaga ttcacgaagt
    50521 gaataaataa caaaccttct cttttctttg tgttctctgt gctcttcgtg gttaattctt
    50581 ttacatttct cttaagtaga cagcattggg cgacggaacg aggcacgaag ttgtgatctc
    50641 taaactaaaa gtaattttcc attttagaaa aaaaaagata ttcccaaata gaaaagataa
    50701 agctcttttt ttgaatgagt tgctatttcc tttcggcaat tcgtatattg cctttttgtc
    50761 ttctttttta aaggaagcag agaggatgtt tcctttaaag ttttggagga gagtaaaaag
    50821 acagaatctg aatttaaaaa ccggtagagt attttgaatt taggtgtaag gcatttattg
    50881 attttcaatt gctcatacgt gttctttcat ctatcgcatt taattcggaa tcaaagaatc
    50941 attttttaaa ataaaacaaa aaaaacttta ttttaaatag aggaattaaa ctagtgaaca
    51001 catgacagaa tttttttcaa aacatgctga tttaattggt gttttattta tgccattaac
    51061 ctatggattt gttggttggt ttacaaattg ggtcgcgctt aaaatgactt tttatcctat
    51121 caaattttgg ggaatccctc cttatctagg ttggcaggga atcattccaa gaaaagcaca
    51181 caaaatggca aaccgtgctg tggacgtaat cacaggtcgt ttgatcaaga tcgaagaaat
    51241 ctttgataag gtcgacccag tcatgataga aaaggaatta aaaccaatgc tcaagcctgt
    51301 cattcgcgat ttaacaaaag atatcgtaga tcaaataaga cctggtcttt ggcctgctat
    51361 accagactta gtaaaaaatc aaatttatgc agaaacagaa aagcagattc cgggtgcgat
    51421 taaaagtatc attatgggga ttcgaaagaa tgtgtatcaa tatttcgact taaaaggaat
    51481 ggttctaaaa agactttcag gggataatgt tgtgttagtc gttgaattat ttcagacagt
    51541 tggtggacct gaatttagat tcatagaatt gtctggtctt tattttggct ttcttttagg
    51601 tttagttcag attggaatct ggttggcatt tcctgtttgg tggactcttc ccattcaagg
    51661 agttattgta ggatatttaa caaactggct tgctttagaa atgatctttc gaccatttta
    51721 tgagaaggat tatattctat ttaaatacca aggaattttt ctaaaacgac aagctgccgt
    51781 tgcagaacag tattcaaaat ttgttgctac aaagatactc aatgcaagaa atatattaga
    51841 agaaattctc tatggtcggg cagctgatga ggtttttgct ctgataaaaa aagcagttat
    51901 acgatctttt gaaaatacat cccagttagc tcatcca
//
LOCUS       DAOQOS010000024        51869 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_250_length_51869_cov_6.450149, whole genome shotgun sequence.
ACCESSION   DAOQOS010000024 DAOQOS010000000
VERSION     DAOQOS010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 51869)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 51869)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51869
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_250_length_51869_cov_6.450149"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..279
                     /locus_tag="PLF66_07235"
     CDS             <1..279
                     /locus_tag="PLF66_07235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63869.1"
                     /translation="LHYDSFTDMKEFIIKNSELMVMDDTGPPVRDLAKDYNIKVFGRY
                     TRPIGLWPEKVQKELQKLHEEQKPEKVSFKYGYGTADKQQHIMVVTRK"
     gene            complement(282..623)
                     /locus_tag="PLF66_07240"
     CDS             complement(282..623)
                     /locus_tag="PLF66_07240"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HMZ63870.1"
                     /translation="MLVNWVRIEAFIDKSSEEDLLWLKTSIADLIQNLDGKRKQINSL
                     MANSDYSLIQPILHQIKGVAANFGLEDFCNVAREAETLLKENQIKEFLSIMDRLSTLW
                     ESTKSELETRL"
     gene            complement(727..1233)
                     /locus_tag="PLF66_07245"
     CDS             complement(727..1233)
                     /locus_tag="PLF66_07245"
                     /inference="COORDINATES: protein motif:HMM:NF016382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease V"
                     /protein_id="HMZ63871.1"
                     /translation="MKPITIAVFDVYYFEAYANGCCVVFKKEKEEKVLSEYSKKIEET
                     SEYIPGEFYKRELPCILRTFDLIEEEIDIIILDSFVWLEEGKKGLGGYLYEALEKKIP
                     VIGVAKTYFRSVSSYKELYRGVSKKPLYISSIGIDLNYAFDLIQSLEGEYRIPNVLKR
                     VDQLSRQI"
     gene            1512..2027
                     /locus_tag="PLF66_07250"
     CDS             1512..2027
                     /locus_tag="PLF66_07250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63872.1"
                     /translation="MNVALGTLISSLDNLVKNKNAANKKTSDAAQNAGVESPIFSASI
                     QARYQVLQSRLSKLQNDLSREQARLGILEKENIQPEEISNILFGATPLFTESLDEVIQ
                     EKQQLVEKINAQKRFLTEQIRSVEIQSENIHSIGLGNGDNVEEKLGMLKKSQPPVINI
                     SQKSVERLIKD"
     gene            complement(2017..3144)
                     /locus_tag="PLF66_07255"
     CDS             complement(2017..3144)
                     /locus_tag="PLF66_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020766333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HMZ63873.1"
                     /translation="MIAYIERIKSEFFPIRILDKYLFLEFFNTFIGTLIMLVGILIVS
                     QVMDNMKTFIASKESSVHIYLFLFYNLPKMATLVIPMSLMFSVCFVVGQFSTNKELVA
                     SMAAGVSFYRIVTPIFIFGGFMWIFMLFLSEVVVRPFNSLAAYEYSLVQKGVGTKTDL
                     VYQLHIKGKEGFYYVYWYDAPTKSVRGGFNYIKLNEKNLADYVISAQNARYNESEKNW
                     KLEKIEEIYFDEHMKVKSYTRYGEKIYTFPESADYFSKPRKKVEEMNIFELSEEIVVR
                     KNKGVPYADLEVERHAIFAVPIMCLIVVIIGAIAGAYTTKSAGVMSLGISVGVVFLYY
                     IMYSTGKSLGENGGIPPWVAVWSTSIFFFGVSFYLYKRFNL"
     gene            complement(3176..4045)
                     /locus_tag="PLF66_07260"
     CDS             complement(3176..4045)
                     /locus_tag="PLF66_07260"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016549906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HMZ63874.1"
                     /translation="MKLIPCNSCQNESFKTIFEKESSQKEIFQIVKCKNCGLIQVNPQ
                     PTFEEVAKYYSDEYFTKRTDRGYDNYYSEKVRTEIERVFRLNLEDLGFFEWEKSLPSS
                     KRTIDIGCAAGYFVNYMKSLNWQAEGIEIAEGPVRFAKEVLKLSITKDDFLNWDIERN
                     EKFDLITLWASIEHLHKPKETLEKIFSHIKPGGRMILSTCRYGILARLTGIRWRYLNV
                     PEHLYYYSLKGLISECEKIGFKKISHITYGSGLTAKKDASIFYKVAKKFLDRFVKWTD
                     QGDMMAIQFIKKE"
     gene            complement(4424..4723)
                     /locus_tag="PLF66_07265"
     CDS             complement(4424..4723)
                     /locus_tag="PLF66_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004424768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HMZ63875.1"
                     /translation="MKHFIIRLDYKVPIEKIDTVLVEHRAFLDSGYEKKILLSSGPQN
                     PRDGGILIARAESLEAIREFCSKDPFAKNNFADYHFTEFIPVKFQKEFESWFLAE"
     gene            5346..6359
                     /locus_tag="PLF66_07270"
     CDS             5346..6359
                     /locus_tag="PLF66_07270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63876.1"
                     /translation="MKLADYFKISLKKPEITESIAQVLDKKNKLKSQIETCFSRLESI
                     EICNSNSKSADAMILSNYLIVDLTNLILIYLNQPTLLSSDKWQDKTKTFPEELSSIFS
                     EHNSILNSSYETDEERSEKIESSLSDILLASEKFIFKKNKADFQNPIDDYKKRSAVQF
                     ILSVIILIVAINTGIKLFNKYKAVKEDTAKIYFMNTANPAPNPNNTITAKVIPSENWV
                     EANFVLPNPVDIKEIKVELIHQVFTRFQLKDLKFLDENKKVLRERNFQLNNLGMVENS
                     EKNEICCTEELKPGKLIPGKYMELETTSSSPSFYVKMEESKSVKEIVLTYRYIKNTNK
                     FPD"
     gene            6385..7269
                     /locus_tag="PLF66_07275"
     CDS             6385..7269
                     /locus_tag="PLF66_07275"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004430253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HMZ63877.1"
                     /translation="MFKQYIILLRIHQWIKNTILFAGVIFGHKLSDPEAILKSFFAFL
                     LFSVIASCQYVINDYLDRKEDSLHPEKKHRPLASGKINPSLALLITAIILPLSLTLCY
                     MLHPYFFGLCSFYFGFNLVYSKYLKHIVILDVMSISIGFVVRAISGAVVINVSFSSWL
                     LLCTFMLALFWGFSKRRGELILLEKSASSHRKILEEYSPQFLDLMMAIASTMTLISYV
                     MYTISPDTVQKMGTDKLVYTIPIVVYAIFRSLYIIYIKNMGHNPSKAILTDLSVLASG
                     LIWVVMVTIILYFKIPSV"
     gene            7269..7775
                     /locus_tag="PLF66_07280"
     CDS             7269..7775
                     /locus_tag="PLF66_07280"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HMZ63878.1"
                     /translation="MKSIRKIIEYAFYIIIFLAITFSIAFFRSGSRKLILDLSKYKPI
                     NTELAKDFESKNKVIYFWATWCGVCETNLPMFKTSYRLFNGKWNTEFISIEEGSSTPE
                     YVKKYLDKNEILYPTIIADSNLLQSNQISGYPTIFFVNSQNEVKLIDTGLMSPVSMFI
                     RILFLSLG"
     gene            7779..9005
                     /locus_tag="PLF66_07285"
     CDS             7779..9005
                     /locus_tag="PLF66_07285"
                     /inference="COORDINATES: protein motif:HMM:TIGR04267.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEXXH motif-containing putative peptide
                     modification protein"
                     /protein_id="HMZ63879.1"
                     /translation="MFSIPELKKLKKELLHSLIKEANQYLPQIPKKYKKDSEKVIKAY
                     IDSESIAGLEIWVNSIAEFLYFEEAKRDLKEIRNNEADLKEFHKAFINSLKQDFGVED
                     FRFIDWERAKPNYKNLSNLFENFSEIALKPCYTIDHNVYLKEESNYSYYMRANGDPSQ
                     KGVKIKMTRTKTPKEKMDQIQKAHEYLETFWEEGFQLYRLLTDKVHIVQSNGLVSYSH
                     FHEQGISYINFIDRDILESIDDLIHENSHHHLNLILKKYKIVKKEFKDDIFYSPWRKT
                     LRPLYGILHATFTFSYAALLFYNIAKSSQWTFLDIDQKFLEKAYFRFIEETLYVEYSL
                     YDLRFAIGKDLFTAKGIELIESLDKYNRDCLLYLPDVLKKIKSKELKKEIKNIQKVLH
                     KNRTQYKINSTQQLSK"
     gene            9261..9610
                     /gene="ssrA"
                     /locus_tag="PLF66_07290"
     tmRNA           9261..9610
                     /gene="ssrA"
                     /locus_tag="PLF66_07290"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(9685..10836)
                     /locus_tag="PLF66_07295"
     CDS             complement(9685..10836)
                     /locus_tag="PLF66_07295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63880.1"
                     /translation="MEKELSFQYNTIDSVIVKIILSIIGFIFFIVGLILVFLGLASIG
                     TEGNLLDVLKFIIPGITIFLFISTAYLYSMISKKRKIVSAYFDLESNRFYMLTKSGES
                     GYIPFGEISSFDMRREVVSGSESSTTYYVVFFIKKDGAWWDIVHFDTELAARKKLNLL
                     QDIIKLDSAEDYNLLDEEKKSKAFLFSETSNGSLFKWKENVSMLYRVIGLLAVISFLF
                     TFGIIVSITNEEGSIGRYVVAVFISIFGIAVLFGLYKSIFGYSEYELAIGLEKVSFFG
                     VKKDKRKLLAEMFIKDIQYSQYTFNMLRNDLFNGQEIYILNSEFAEKIEKYKKGDLGI
                     GEVIGFLKDVMQMQKNIIKLPFPGFMSLDIILFEKKLDEELRKRNPDLK"
     gene            complement(10987..12186)
                     /locus_tag="PLF66_07300"
     CDS             complement(10987..12186)
                     /locus_tag="PLF66_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020771234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63881.1"
                     /translation="MNAILTRIKRAFPFLISTLFLVCDDIKRPPLSNLFINSLLVIPS
                     AGVERVGVVTSDFGTSGKFAVINPDTQLSITTIATIHSDAVAKMIDGKIFVINRLNRD
                     SILILNPANGFLPESEYSVGKGNNPQDIIKVNENKAYITLYNSRNLLVVNPYSGAFIR
                     NIDLGIYAEQTSLGAAGSDGIPEMSQMFLEGNLLYIQLQRLDRNDPTGIPSPNSDSLL
                     LELDVNTDLVNRAYTFKSRNPTSRIQKITIGGEAFLAMCAPNRLGFISKLDGGIVAFS
                     LVTKTFRSNFLFSETEAGGDILAMQIKDENEGYASVLDSSFNKFIFQFNPMTGQRIKT
                     ILNFPASAGNISGLLLSSSGKLYMGDGSFSKPGVLIFDTTKPDKPRLTATSIDVGLRP
                     FDLIEIR"
     gene            complement(12196..13128)
                     /locus_tag="PLF66_07305"
     CDS             complement(12196..13128)
                     /locus_tag="PLF66_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015681663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HMZ63882.1"
                     /translation="MQLRLVGVKSKVGFHILLIVLTFLTLTFKEGVWAILFAFEENKE
                     ILTKIFWTEYPYAVSLLVILFCHEMGHYIPARIYNLKTTLPYFIPMPASPVGTMGAVI
                     QIQEPIQDKIKLFDIGVGGPVASLVLSAICWVIGLYYSNLVDLAEFANQKNILNFGDS
                     LFTYYTGQWILGPYDPVNTTILIHPLAKAGWVGLLITAINLIPFGQLDGGHIVYALFG
                     EKYRSWIHYLFLFFLLLGLVSFTWVIWGFIIYYFIKIEHPYVPDSSTELGLGRKFLGY
                     FMLASFVLIFVPIPLSSGETMLIQDIFNYIQQSF"
     gene            13195..14994
                     /locus_tag="PLF66_07310"
     CDS             13195..14994
                     /locus_tag="PLF66_07310"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HMZ63883.1"
                     /translation="MLNLKDLSIKSKLILSVGIFQVLSLIIAILGTMGLSRMEKELLI
                     VSDIFVNIEVNLNDLKFTVDSRRLALLRALNTDSQAEFDKEMTLVKKYESEFKDNIPK
                     IRKFLQENASRFETIKKKPEDSDLKDFFNSLDEVEKLLETFASATLIYEQKMSEAFRY
                     DTPDWRKLQLKKESMEMYSERSLNHTKFIDKIKQLNQFTANGLGQSTQGVIGTSYQLK
                     LVLWIGYAISLVIVVIVTYMMIRRIAYPIISAEKMANRFADGDLTIYIENNSNDEIGR
                     LVRGLNKASENLKNVVKQIGNTSDHLASSAEELSATSRNLADGATNQASSLEETASAV
                     TEITESISYVSESARDQEKEVIETNKSMNELSKSIIQITETARAVNEGSQSALAEAKE
                     GQVKVNETVKRMAAIEESSEQISDIINVINDISEQTNLLALNAAIEAARAGESGRGFA
                     VVAEEISKLAARSQKATREIAELITDSITKVNDGKLIVGQVVESLNKILHKSMEAAQL
                     SDSISNATKLQSSGNQKVLKSVTTLSQMAQSISRATNEMKISSKEISNAIEQVNSVAQ
                     NTASSSEEMAASTSELAAQSEKLSQLIGVFKTN"
     gene            15102..16208
                     /gene="tgt"
                     /locus_tag="PLF66_07315"
     CDS             15102..16208
                     /gene="tgt"
                     /locus_tag="PLF66_07315"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020775491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HMZ63884.1"
                     /translation="MIYTLKKQSNDSFARAGELNLNGIKIETPVFMPVGTRGTIKAIT
                     SEDIRELGLELILGNTYHLYLRPGTEVLDSFGGLKKFMSYEKALLTDSGGYQIFSLNG
                     LFKYHKDGVKFSSHIDGSKHEFTPEKVIDIQRSIGSDIMMVLDDCPPYGADIERLEEA
                     LTRTHYWAEKSIRHFEKNRNNQNLFGISQGGVNLDLRIKSLEFIQSLPFDGIAVGGLS
                     VGEPRPEFVRILEGISSKFDPQRPHYLMGVGTVTDILEGVKNGIDMFDCVLPTRNARN
                     GQVFTSYGKVNLRNEAHRMSDSPIDPKCNCKVCKNYSIGYIRHLHKVKELLAFSLSTY
                     HNLFFMKEFMNGIRQSIYNSNFLEYYLYWKKLYD"
     gene            16312..16647
                     /locus_tag="PLF66_07320"
     CDS             16312..16647
                     /locus_tag="PLF66_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000406877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HMZ63885.1"
                     /translation="MEITRRENKNIIILDINGEIDLYNAPEIKDIIAKLIEEKRYQIV
                     INLEKVSYIDSSGIGALISSLSNLKKYQGGLKIINVAGSVRKVFELTKLTSFFEIFDA
                     EADAIGAFK"
     gene            16672..18030
                     /locus_tag="PLF66_07325"
     CDS             16672..18030
                     /locus_tag="PLF66_07325"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HMZ63886.1"
                     /translation="MRENSSNIKIFFLPYLVFCVVSCGATLPLQEITTAKQEISKAKL
                     FSADKYSKKEYDESKTNLYEAHESVSSDKPDLKKASQLAENSAEKARLAIQKSLPEYI
                     KDLRKESISSLDAANQALADSLAADLYEQANLLNIEADELTKNADSRLKNTNNSSVNS
                     NVYDDYEKGIKRYKESISLSEKARDLSLAQTQAIIDSTTDIETNLDLVEKYAKNDKSV
                     LSNVKNLRGEYKSSIDTMESGRLREGFKKTEEIRNRSNELIASVILPYAKDRIKLATT
                     KIEEAEKNINSEEKEENSPSINKDNLSAAKEAFNSSVDLLGKERYYDSIQQSDESIRL
                     AEEILKSKTNTLANKSRATEEKSDKAIEDADARDSFPRKNKAKTTDPKADIKDGWKKY
                     IVKKKVPPDTLWRIAANKEYLGNKDKWKEIYKANSKTISNPNKIYPNQVILIPPIQKK
                     KK"
     gene            18306..19178
                     /gene="nadC"
                     /locus_tag="PLF66_07330"
     CDS             18306..19178
                     /gene="nadC"
                     /locus_tag="PLF66_07330"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016755639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HMZ63887.1"
                     /translation="MDRFYTTPVEKLEATDFKNLVRLALEEDKPEQDITSEAIFEKDQ
                     MATATLIAKESGILAGSIVLEELKDQTGHLFSYETFKKDGEEFKPKDKILQIKGNLIV
                     ILRIERILLNFLQYLSGIATEANKISSKYKNRLMIFDTRKTLPAYRKLAKYAVYIGGA
                     CNHRQNLSDMGLIKDNHVAMSGSIEEAVKKIRTKFPNKKIELEIDGLFQLDDAINSSP
                     DIIMLDNFTVEDTKIAVEKILKTKPQIRIECSGGITPEKLNSLSEIGSIGVSMGYLTH
                     TTRFLDLSLEIEQN"
     gene            19178..21211
                     /locus_tag="PLF66_07335"
     CDS             19178..21211
                     /locus_tag="PLF66_07335"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016760136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HMZ63888.1"
                     /translation="MGFLKKEVSVEDLFEAVKERFSPENLDLIKRAYDVSNRTHADQK
                     RYSGEPYVIHPVNVAYILNDLKQEPAVVAAGLLHDVIEDTSYTREDMTNDFGKDITKL
                     VEGVTKMSKVKNKTRMNFESIAAENLRRMLMATAKDPRIIVIKLADKTHNMRTLKFHK
                     PEKQKIIAQETLSIYAPLAGRLGVYKIKSELEDLAFQILEPEKYQEIKEKVASKKAER
                     DSFIETAKIIIKQRLAEINLDVQIEGRSKHFYSIYKKMKDKDKTFDEINDLRAIRIIT
                     KEVRDCYGVFGVVHSIWTPVPGKFKDYIATPKTNQYQSLHTAVIGQDGRPFEIQIRTA
                     EMNHIAEHGIAAHWAYKEGTKSSKDVAMTAKWIEKIRAWSESSSDSKEFLEDLTHELH
                     EDEVFVFTPKGDIYNFPKGSTILDYAFRIHTDVGIHAKGAKIGGRMVPLRTELKSGDQ
                     IEVITDKKVKPSPIWLRIVKTSSAKQKLRQYFRKVQDETASETANAYAAKTVVADQPL
                     PEIDLTQLKKLKPDKKKNKEKREISIVVAGIKDILVRLSSCCSPLPGDEIVGFITRGR
                     GVSVHKANCDVALHYSDKKRIVAVRWDGVNNPVPVRIEVKAYDRQGIYLEMVESISKT
                     ETNILEAGASSSGESTLLARFLIEIEHLDQLQEILDNIRSIKNVLYAERVMEK"
     gene            21282..22160
                     /gene="rfbA"
                     /locus_tag="PLF66_07340"
     CDS             21282..22160
                     /gene="rfbA"
                     /locus_tag="PLF66_07340"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HMZ63889.1"
                     /translation="MKGIILAGGSGTRLYPVTRVVSKQLMPIYDKPMIYYPLSTLMLA
                     GIREILIISTPNDSPLFRALLGDGSDFGIKISYEIQPSPDGLAQAFIIGEKFINGDNS
                     CLILGDNIFYGHGMIENLVDATKLETGAVIFGYYVNDPERYGVVEFDKTGKVLGIEEK
                     PQKPKSNYSIPGLYFYDKHVCEYARNLKPSPRGELEITDLNKVYLQKGQLTVNLLGRG
                     IAWFDTGTHNSLLEASQFIEVIENRQGLKVGCLEEIAFRKKYITKEHLLKLAEPMKKN
                     GYGQYLIRLANEHSID"
     gene            22141..22689
                     /gene="rfbC"
                     /locus_tag="PLF66_07345"
     CDS             22141..22689
                     /gene="rfbC"
                     /locus_tag="PLF66_07345"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HMZ63890.1"
                     /translation="MNIQSTEFDGLYIIEPKVFKDDRGFFYESYSEKHFADKGLKFNF
                     VQDNHAKSQDPGVIRGLHYQKPPFDQAKLVRVTKGAVYDVVVDLRQSSKTYKKWFGIE
                     LSGGNFKQLLVPRGFAHGYLVLKPDTEFLYKVDNIYSKESEGGILWSDPDLQIDWMVN
                     KPILSEKDTKLPLLSESLGIFP"
     gene            23250..26018
                     /gene="ileS"
                     /locus_tag="PLF66_07350"
     CDS             23250..26018
                     /gene="ileS"
                     /locus_tag="PLF66_07350"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020768967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HMZ63891.1"
                     /translation="MKEDKKQENPYSKTVILPTTEFPMKADLSKREPQTISKWKKEKV
                     FTQMRNLRKSEKTFVLHDGPPYANGNFHVGHALNKILKDIIVKSKHLAGFHTDIIPGW
                     DCHGLPIEVQVLKNLGKDAANTSSTDLRKKCRDYANEFVGKQGEDLSRFLCFFDEDRK
                     YLTMSPEYEAKIIEVFGDLFSKGYVYKGKKPVYWCIALGTAHAEAEIEYANHTSPSIY
                     VKFPVIGESDTSCLIWTTTPWTLPANLAICFNKEIEYSIYSSDAHGKMILADGLKESV
                     EAKCEIKLTKVKSISSDELAKLKFQHPFIDRISIPLFGNHVTLEAGTGCVHTAPGHGM
                     DDYKVGLAADLEAFSPVDDRGKYTHEFPLMEGVQVFEANPKIVELLKEKNALVHYSTF
                     EHQYPHSWRSKKPLIFRATPQWFFKIDEKDLRKKSLEAIDKVKWIPDWGITRIRSMVE
                     NRPDWCLSRQRNWGVPIPAFRLANGETFMTPETVQYFVDIVKKEGIEVWYTREPKDLL
                     PPNIKLPFGDVKDVIKENDILDVWFDSGVSNFAVLEYKGDSPADLYLEGTDQHRGWFQ
                     SSLWPSMALRDKPPFKSVLTHGYVLDEKGHAMSKSLGNVINPTTDIINVYGADILRLW
                     VSSQDFRDDVKIGKESIKNVSEQYRKIRNTFRYLLGNLAGHTAADNLSYPEMQEIDQY
                     YLALLYNLSVELKNSYETYQFHQIYQKVLKFCVSELSQDYFEIIRDRMYCDRRDSKTR
                     RSSCTALSIILETLVVLLAPVLSFTAEEVWENYGKKTSVFLEKFPDLSQFKNEVLIAK
                     FEKVFETKELVQKALENARQNGKIGKSLEAKLFISYKNTSILEKLFSREDFELFYVVS
                     QVEFEPIGNSVTDVGDAYMPPLLSSAENEDFLVEVTLPTNPECPRCWRHTRDIHKGGL
                     CERCKAAV"
     gene            26151..26510
                     /locus_tag="PLF66_07355"
     CDS             26151..26510
                     /locus_tag="PLF66_07355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63892.1"
                     /translation="MPQYIKPKKNEIYLDTFQELYPKFNSEIKTKEFNLIFEYLVDNI
                     DKLVWAIEDLDSPPLEGVGKALVSLNPKIFNNPDTRRMTGALIAAILHHHGYMPATSG
                     VRVSWKPFITASKYKKK"
     gene            26608..26898
                     /locus_tag="PLF66_07360"
     CDS             26608..26898
                     /locus_tag="PLF66_07360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63893.1"
                     /translation="MEYLNKYQLMLKLKDFIEIHEKTKISDLNEGVLKMYILHSKNQN
                     NTPKEKLINYKLLRIDERLLNNSPEEISERDSIIVEFLIEELMKYYARSKKK"
     gene            26913..27734
                     /locus_tag="PLF66_07365"
     CDS             26913..27734
                     /locus_tag="PLF66_07365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63894.1"
                     /translation="MTEILSQEEIDALLDAIGMDPEPQIDTLQDLSNYLIQKRIEKKK
                     NSFEEQYVNLFQGPHIQVTKSLINNVYTQAAVKEPKAIVKIGNIEIIPINICPTCNNY
                     HYDEEVQEIYSESKPDKSFISKNKKLKEYDLIKKWKMENRTICCKKCKTYFQPTIVYS
                     KTHNSIPYFCKLQTVKTLDDWFLTERKSKIQLFSLKTKTIKINKNRTSFLWNVFLNKN
                     INGMETCPQALLTNIVRYTPIEHLEKMLRNEKNIPVFNSFDFTPYYKPTQIFNEI"
     gene            27886..28623
                     /locus_tag="PLF66_07370"
     CDS             27886..28623
                     /locus_tag="PLF66_07370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63895.1"
                     /translation="MNFELYYGKPGKIDYDTIINMFPKNSINSVRTSSLPLLVYWKDT
                     DKKLKKILSFLKLNSSDKYSLCFEYPTSSGAKGKASMTDLMILSDSKKIAIEAKFTEV
                     TGKYEVIENWLQNPNKDNRKNVLENWIQMIQPFSSSELKIENIKTIPYQFLHRTASAC
                     AYYESKEVYVIYQIFYDGETKAELEKFKKEIIKAKEIINPNEKLKFYLWELEVKQCIE
                     EADKEYAFEQIKTKNIYEFLRDEIVEI"
     gene            28686..28901
                     /locus_tag="PLF66_07375"
     CDS             28686..28901
                     /locus_tag="PLF66_07375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63896.1"
                     /translation="MTKLTLKIIYLFIAILLISCGNKMAGKWNCTGGMVSGLEFLSDD
                     QVILGIMGAKTTTTYVKNKDMINIKTD"
     gene            complement(29004..29393)
                     /locus_tag="PLF66_07380"
     CDS             complement(29004..29393)
                     /locus_tag="PLF66_07380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63897.1"
                     /translation="MEIIFYTKNGKQYVLEVSDSFYKWLALSEFSKIQTSNVYKVEIE
                     GDETEEIQAIELTELNRNLYISFFQKCILSESYDLVLKLESSSKPGLLKASSLDTSIE
                     DLQYRIKKFYEFLKLLEDRDWVYMEYV"
     gene            complement(29396..29599)
                     /locus_tag="PLF66_07385"
     CDS             complement(29396..29599)
                     /locus_tag="PLF66_07385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63898.1"
                     /translation="MPYIEIKTREKINRGLALTLIEKGTISAVVTTGTISPNAKKLFD
                     EAGIAWAENIPTSEFANVKSMEI"
     gene            complement(29599..29826)
                     /locus_tag="PLF66_07390"
     CDS             complement(29599..29826)
                     /locus_tag="PLF66_07390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63899.1"
                     /translation="MMDQNKPTDEDKMIQKLTVHKNLISWMLTLFQKENIESERTYKN
                     DPNGDIYYPNEKNTEKVKEIVRDLNRKYNKK"
     gene            complement(30045..30377)
                     /locus_tag="PLF66_07395"
     CDS             complement(30045..30377)
                     /locus_tag="PLF66_07395"
                     /inference="COORDINATES: protein motif:HMM:NF014043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HMZ63900.1"
                     /translation="MNESDEIRLQHILDAIDDIESFTKGISLTQFKEHRLIHNATVRC
                     FEIIGEAVAALSDEIRNTDTNFPWQDWKDFRNVLIHQYFGIDYELVYNTILQDLPDLK
                     KEVSRLIG"
     gene            complement(30364..30657)
                     /locus_tag="PLF66_07400"
     CDS             complement(30364..30657)
                     /locus_tag="PLF66_07400"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HMZ63901.1"
                     /translation="MLIEDSTIEIVCAFLAKTETKKASVFGSYARGDAEESSDLDLLV
                     EFEPTISLLTFVRYKRELEKLLGKKVDLLTPDSISPRIFPSIKKDLKLIYERK"
     gene            complement(30661..31755)
                     /gene="mtnA"
                     /locus_tag="PLF66_07405"
     CDS             complement(30661..31755)
                     /gene="mtnA"
                     /locus_tag="PLF66_07405"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HMZ63902.1"
                     /translation="MKTQRLILKSIDWKGDRLSLLDQRKIPLAKEYIDCMSLEDVIFC
                     IKSMVVRGAPAIAMSGIFGLVLELKNAREKLEYEKFIRRTRLLLESRPTAVNLRLAVE
                     DYLKLINEKTYSTNSISDLIKLSEKYAISLLEQDLHWNLEIGKNGLKLFSKRQKEISI
                     LTHCNTGALATAGHGTAIGIIRSLRDAGYKITVYADETRPYQQGSRLTAWEMQEEKIN
                     CFIIAEGMSAWLFQNRKIDAVIVGADRIAPNGDTANKIGTYALSIVAKENKIPFYVAA
                     TKTSFDFTLKDGSEIPIEMREEEELTQNSFLKDLRGNSYLPKGILAPLKARALNPAFD
                     VTPAKYISAIVTEHGIIQPVNKKEIKRIVG"
     gene            complement(31758..32039)
                     /locus_tag="PLF66_07410"
     CDS             complement(31758..32039)
                     /locus_tag="PLF66_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HMZ63903.1"
                     /translation="MFKVNEYFEGKVKSIAFKTSEAPATVGVMAPGDYEFGTSQKEIM
                     TVISGQLTVKLPGASDWKDFGKNQSFTVEANQKFQLKVKEDTSYLCLYL"
     gene            32417..33988
                     /locus_tag="PLF66_07415"
     CDS             32417..33988
                     /locus_tag="PLF66_07415"
                     /inference="COORDINATES: protein motif:HMM:NF024921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HMZ63904.1"
                     /translation="MNKNLLNFLLVFTLTIPFLQNCHDKEKDLVILSGSENESIEPIL
                     KNFESETGIRIEMKYKGSVDIMMELGNETSNFDAIWPASTLWISLGDKSRKVKHIKSI
                     MTSPVAFGIRKSLAEELGFAKRDVRVKDILAAIRTKKLKFIMTSATQSNSGTSAYLGF
                     LYALLGNPDVITEEDLSKESLRKDMKELLNGINRSSGSSGWLKELFLSGKYDAMVNYE
                     SLLIETNQTLVSQGKEPLYIVYPKDGIVISDSPLGFVNSGNPKKEESFKKLQDYLLSE
                     KIQKQILNTGRRVGFGGTIEDGDKSVFNPNWGIDSKRILSPIQLPSADMIRKALSMYQ
                     TEFRKSSYTVFCLDFSDSMLGTGERQLKEAMDLLLDKEKATRYFLDFSKDDKIVVIIF
                     SHITLAMWTANGNNPDEIKELNAKIQGTLPNGATDIYSPIIAALKELKDVDNDEYSPA
                     VIVMTDGISNSGKNFKDVSRAFKKIDKDIPVFPIMFGDASEDQLKEIAELTRAKVFDG
                     RSNLIQAFKTAKGYN"
     gene            34152..34784
                     /locus_tag="PLF66_07420"
     CDS             34152..34784
                     /locus_tag="PLF66_07420"
                     /inference="COORDINATES: protein motif:HMM:NF021617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HMZ63905.1"
                     /translation="MKDFIKDKITVKGNLFAGFTGGGVFSLFFLFLNIPLGASITASI
                     VSYLAGLLIFAKANQQFDIKLSDVKIDDKRSGEILQEFRRKTNQLRIHSNSIRNSKIK
                     AKVEDILSVLQKLDDNFIKDPSDIARARQFLVYYMDSTVKIVQFYIDLSAQNSNSKEV
                     KDALTKGESILDLILHSFEVQHAKLLSNDVMDFNAEIEVLEKTLKMENME"
     gene            34784..35860
                     /locus_tag="PLF66_07425"
     CDS             34784..35860
                     /locus_tag="PLF66_07425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63906.1"
                     /translation="MDDLNKIIGIIVLAVMIGGGVAYKMYDSGSDAVVIRGFIGGEKA
                     NFLENEKVKKILKNKYKIILEYSKSGSIEMVQNTPSDNTDFLWPSSQVALELFKINHP
                     QKIVKSEIILNSPIVFYSWDLVTDALIKDEIVSKKGNSYYITDFKKLIQNISNGKKWS
                     EIGLKELYGKILILSTDPTKSNSGTMFAGLLANLINGDTLDETSFAEVSSSVKDYFAR
                     LGYMETSSSDLFEQYLKTGMGAKPIIAGYENQIVEFSIEHSDFWPKVKEKIRILYPVP
                     TVWSSQPLIILNPKAVNLIQALQDKEIQQIAWEKHGFRTGLIGQQNDPKILEVVGIPE
                     SITQVIPMPSPTIMEKLIKVIQSN"
     gene            35968..38448
                     /gene="hrpB"
                     /locus_tag="PLF66_07430"
     CDS             35968..38448
                     /gene="hrpB"
                     /locus_tag="PLF66_07430"
                     /inference="COORDINATES: protein motif:HMM:TIGR01970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HMZ63907.1"
                     /translation="MNLPIFEVLDELKANLAKTNLVILQAPPGAGKTTVIPLALLEES
                     WLKDKSILVLEPRRLAAINAANRMSKSIGEVAGGVVGYRIRFDSKVSNKTKIEVVTEG
                     IFTRRIQSDPELKNIGLVIFDEFHERNLQTDLGLAFALEVQGAYREDLKILIMSATLD
                     GNHLSNFFSAPLIESQGKAFPVEVKHLGLSKEKLNSSILTAIQKALKEKGDILVFLPG
                     SGEIKKIQREIEENLNLTDTLIYPLYGDLSQKEQELAIFPDKSGKRKIILSTNIAESS
                     LTIEGVSIVIDSGLARVPRFDPSTGMTELVTIQIAKDSMEQRTGRAGRLGPGISYRIW
                     SKEEERNFSEKTKPEILETELSSFLLEILNWGSRLKDLKFLDMPSESSVSRSKSLLLS
                     LNLIDKQEKITDLGKEINRIPLHPRLGYMLYQTKDTKEFYLACLIASILTEKSLFTAN
                     HFSFKQSDIALEVEEFLKNQNSYLTQKIKLSTEELMRTLQTESKSKTENIDISQIGKI
                     LSFAYIDRIAKQREKNSNRFKLSSGKGAFLRENDSLIGNEFLVVASLDGDKKEAAIFK
                     AAKISLEEILSTHSNKILKQERAFLEEKSGKILVQEEVVLGELVLSAKEKDSKITEEL
                     LSNLWIDYIKERGLSVLAYTEESKNFKERSEFLRSNGEDLPDLSDEYLLLHLEDWLSP
                     YLISIRKLSELRNLDLLSIFKSLFSYDQIQLIEKEAPERWTVPSGSKIKLEYRKEEVI
                     LSVKLQELFGLKETPTIAKGKVKLTLELLSPSQRPIQITKDLAGFWERTYLEVKKELA
                     GRYPKHPWPDNPLEAQATRFTKNRINQT"
     gene            complement(38435..39742)
                     /locus_tag="PLF66_07435"
     CDS             complement(38435..39742)
                     /locus_tag="PLF66_07435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63908.1"
                     /translation="MAKLLDDLEGQIILNQSDENTVANALKMYLNFDRGSRYDKRILE
                     ETRKVFLDKLSNKSYFFAPPKQNKLKAIGYNQIIHILVKRLWDEEDFFKITRNPSQIS
                     FISTDTADIHTANIKITDVQKFIANQNIFPFISSGDISFFSKSGPDQVRKCLTWIKNG
                     NNMPKFLDRIRLVGGYVQTVFFAAQNLLTVAAEYAANGSSESTESVVAEENSDMSDSD
                     AKLVALTESQGPGAELLNTKLDYPYIVDILLFEIRSLFKNPTSNVNIQEAIKSITLRL
                     KKDIPDVDEKHITLIYLIMKEVAKDPKKTLEDLAFFFQNLEEKLRIPLREIHTIYYLI
                     LSEHDNTLQEMPLSEFVDKCIAEDEKGILTETHIKILKSLFGTLPKEIKANTFYTVKE
                     KIKARIEDMNKKKMVDTLSELIHLRVREFRGRFALNKLKSKSD"
     gene            40095..40454
                     /locus_tag="PLF66_07440"
     CDS             40095..40454
                     /locus_tag="PLF66_07440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63909.1"
                     /translation="MENIPQEIIDRINTLKQDQKEKVIKYIKREIEVGRIPSVDELSS
                     IIRYLNESEKKGTLYKIEGIAIEPDKPKKTLQVTEKSNCLACRFQESGICKNKKSELF
                     ESKVFASIICVHFATKT"
     gene            40456..41226
                     /locus_tag="PLF66_07445"
     CDS             40456..41226
                     /locus_tag="PLF66_07445"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HMZ63910.1"
                     /translation="MSLKTYKDGNKQNPTIIFLHGFPLDHRMWKSQIEFLKDKYYCIS
                     YDILQKLEKSKNPEPIPFEFLVDDFLNLLEKKKIQKPIVCGLSMGGYILLRAIEKKPD
                     AFSKLILCNTKTEADTNEGKLKRVDGIRKINSEGLKDFIKEFTTNALCEFTCKANPEI
                     YKSALKIAKDRTPNATKAILLAMQGRTDTTHVLKTISIPTLVIGGEYDSLTPPESMEK
                     LANQIPNSSFVKIPDAGHFAPFENPKFTNQKILEFLEK"
     gene            41223..43013
                     /locus_tag="PLF66_07450"
     CDS             41223..43013
                     /locus_tag="PLF66_07450"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HMZ63911.1"
                     /translation="MKFILFLFFIILTPIFSEDNLGFKKKQNIDTNELEVFSEKFFRE
                     KKLEGKHVLVAIVKDNKSFFKVYPEHSKAFTELTQFNSVVFDEIFLTLHTLHLEEIGK
                     LNLRSDIYNYKWKYNRNFKQSILIENLLTHTTGIQENLIYSNLEKERPKDFWKNQLPA
                     RFAEPNELISYNPDHSVILKSILEEVNSKSLDEVMKETILSKLELEKTNYENQKLNIN
                     AKDIEKLLFLFLNPDLLAQNDILKKETWNSMFQEKIRLEDKLPGSVIGFFEHYENKIW
                     GYSRIAASNLSTEEFIFFPDKNTSFYIYTDSFDPDLRIEWKSEFLDHFFQNKKVKAKK
                     DKNPGYEEYLKNFEGEYASVSISSQNISKFRNYSSAIQIKQENRLLVLESGEIEPYGD
                     LHGRLEFIEIDPFLFRCLDRESYISFKMNEAGDVEYLLSGSGQHGAYRKLPFHEKKSF
                     QESILYFFIGFYSIYLILLIIEVPYSFAKKIAFVQNRMRRNTMILLWIETLLAISTFV
                     GFYYFIQENKLSQVSEYILTGNSFDYALFTLPLLFIFVLSFLAYYSLKSFLEKGLHLY
                     RQIQTILFLVVSLGFIYWMSYWNLLVFLFN"
     gene            complement(43210..44370)
                     /locus_tag="PLF66_07455"
     CDS             complement(43210..44370)
                     /locus_tag="PLF66_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HMZ63912.1"
                     /translation="MYQAFTEDQIQIRDLVRDFTRKEITPVAHIYDEKNEHPKELIDR
                     MRRELGINGLTIPVEYGGMGYGSVEQCLITEEMSRGCLGIALCFGYTGLGQLPILKGG
                     TDAQKKKWLEPVIAGEHGISFCLSEPGAGSDVPGMMTKAEKKGDKYVINGAKQWITGG
                     GSADAYTVFAYTDRNRGTRGVSCFYVPRNTPGLIVGKKEDKLGIRASDTRQIIFEDCA
                     VPAENLIGRENMGFVYALHTLNASRPFVAAMGVGVGQAALDYASKYAREREQFGQKIG
                     TFQAVQHMLADMSITVEVGREITYRAARMSDAGDPNLAKYSAIAKAFTSEGAMKCALD
                     AVQIYGGYGYTKEYPVEKLMRDAKILCIFEGTTQIQKNEIAAYVTKEAASGK"
     gene            44728..45381
                     /locus_tag="PLF66_07460"
     CDS             44728..45381
                     /locus_tag="PLF66_07460"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HMZ63913.1"
                     /translation="MIRILIFLFLIGLFFCCLQNSKHLNPPTKTIQTYFINSDSGLKM
                     RIEPDQKSKTIHTIFNGEEIIILEEKTEKVIIDNIENSWVKIQNKEKIGWVFKGYLSE
                     KNPYIGKYKMKIVNYAQCKYPEFFLIEENNLWEGTFFIGGDEGDCGYTDINGTWYLKQ
                     NQICFKIKEASSSHDHFYKGIDPCYYLQKNTLYAVNDDYAFKENFGSTIVSGIKKTE"
     gene            45392..45895
                     /locus_tag="PLF66_07465"
     CDS             45392..45895
                     /locus_tag="PLF66_07465"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020768847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HMZ63914.1"
                     /translation="MITIRPANKNDLSLLQKWDEEPHVIESDPNDDWEWEKELDRNPE
                     WREQLIAELDGRPIGFIQIIDPALEDSHYWGDVSSHLRAIDIWIGDKNDLGKGYGSVM
                     MELAIEKCFNDKEVKSILIDPLESNVLAHKFYEKIGFKFIRKQKFGEDNCFVYSLDRF
                     DWENKKK"
     gene            46387..47004
                     /locus_tag="PLF66_07470"
     CDS             46387..47004
                     /locus_tag="PLF66_07470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63915.1"
                     /translation="MNLHKYSILQSSPIFTTHEFALQTNTSMSSAARLLTRIEERKEI
                     NRVTRGIWTYTKEQKVHPFSVTPYLLGKEIGYISLLSALHIHGILSQIPTRIQVVTIA
                     HSRLVKTKLGVFEFFQMKSSMMTEGVTWSDTKLPFRIATPEKALLDVFYLSTRKGKRF
                     YSLPELELKESGFKKKVFPNLLTNQNFSKPIHNAILQRFKNLQNH"
     gene            complement(47167..48303)
                     /locus_tag="PLF66_07475"
     CDS             complement(47167..48303)
                     /locus_tag="PLF66_07475"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HMZ63916.1"
                     /translation="MDRNIYAKDILKALRDFPVVALIGARQVGKTTIARLVKSKYHFD
                     LENPRDLAKFENPQLLLENLSGVVVIDEIQRKPELFTLLRFLVDRPKNKTRFLVSGSA
                     SRELIRQASETLAGRIYYTPVYGFDLSEIKKTDWKRLWERGSFPMSFLAKAANKSYAW
                     REQYITTFLEKDIPQLGITIPAYTLRRFWIMISHYHGGLLNYAELAHSFGISDMTVRK
                     YIDILKNTFMLNVLQPWHENTGKRLTKQPKIYLSDTGIFHTLQFIEKFSQLQAHPKIG
                     ASFEGFVINQIISMQRKELVNYFFYRTHQGTELDLFWMKGGKRWGVEVKYSDAPTLTK
                     SMQIAMNDLKLEKIWVVYPGEDRYMIHKKIEVLPVSQLNELMQK"
     gene            complement(48573..49052)
                     /locus_tag="PLF66_07480"
     CDS             complement(48573..49052)
                     /locus_tag="PLF66_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002762166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HMZ63917.1"
                     /translation="MQTEFKIINIDSKFRTQAIELVNQFFKQINAMKLDGVFKVKSKA
                     ASKMVDIYLKLQGTGKIVFIGAVLDDELVSLLIARIEERPHLEEDKILYIDLAVTKRG
                     QMKKGYMHSLLDFTESWAKKKKIKVIELRALTENRDAVRFWSNRGYSDFYIRFRKLV"
     gene            49100..50275
                     /locus_tag="PLF66_07485"
     CDS             49100..50275
                     /locus_tag="PLF66_07485"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HMZ63918.1"
                     /translation="MKVNQFLSTLTNLEKTRNFNVFKEYSLEEFKSVMNHFQLLESSR
                     NKLRISIVGTNGKGSTAFLLSQVFSRLEGNSPVGLYTSPHLIVQNERIQVDGIMISDE
                     WIENFLNSFHADDLAKLRNLSYFEFFTLLSVFYFKSKECKTEIYEAGLGGRLDATKLI
                     NPEIVVLTKIGLDHTEILGSTEEKILHEKLLISGTNTRFLFTFKQSNKLMEIIKDFCN
                     EKNIGLYVYDKTIQTDYLTFNRDFALFIANEILKTKNKPPLGDTFFNSMHDVPGRMQV
                     LRKNPPLIFDVGHNPSAIPYVLQSLQFLYPNEKNWSVCFGALKDKDVIKILEILTASP
                     IIKKVFQIVGQSWNITTISHKKIKLIQEEDFLDSIPSEAGLILGSFRLYPPVKQNEF"
     gene            50265..50561
                     /locus_tag="PLF66_07490"
     CDS             50265..50561
                     /locus_tag="PLF66_07490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63919.1"
                     /translation="MNFSLSLFFIHLASWFFLYIFPLLLVSIVMITMGLLLRHLIFTA
                     KGNRIRHRKVLTYEEKRAEIEDFSIKITFIFRAFFIGLTLAAIILSVYLWLYEI"
     gene            50605..51072
                     /locus_tag="PLF66_07495"
     CDS             50605..51072
                     /locus_tag="PLF66_07495"
                     /inference="COORDINATES: protein motif:HMM:NF019416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HMZ63920.1"
                     /translation="MGEKGNKTLVCIGINPSIAEPEKLDNTLKVVKRFSKDLGYDSWL
                     MLNVYPQRATNPNELDFEINMDYHRENLFQINKILKHGNYDIWAAWGTIIKKRKYLVS
                     CLKDIYMITRKYSLKWNKIGRLSKEGHPHHPLYLNRNLKLEQFDIDNYLNRLV"
     gene            51199..>51869
                     /locus_tag="PLF66_07500"
     CDS             51199..>51869
                     /locus_tag="PLF66_07500"
                     /inference="COORDINATES: protein motif:HMM:NF013691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HMZ63921.1"
                     /translation="MNFKKTIRIFLLDGEINGRLTCELSNWTGKAYKIPRNKIIASSD
                     RSELLKAGVYMLFGKSEDDSYEGEAYIGESENIFERLKQHLKEKEFWYDSMAIISKDE
                     NLNKAHIKYLENKIFSIAENTKRFKIHNENIPAKPSISESESAEMDEFLSNIKILVNT
                     LGYKIFDETASVSKEKKKSEIFYISATRGAKASGKSVSDGFLVFKNSEIAFSLTNSYP
                     ESMKR"
BASE COUNT        18515 a         8696 c         8460 g        16198 t
ORIGIN      
        1 cttcattatg attcctttac agatatgaaa gaatttatca taaaaaattc cgaactcatg
       61 gtaatggatg atacaggacc accagttcgc gacttagcaa aagattataa tattaaagtt
      121 tttggacgtt atacgagacc gataggactc tggcctgaaa aagtgcagaa agaattacaa
      181 aaactacacg aagaacaaaa acccgaaaag gttagtttta aatatggtta cggaacagca
      241 gataagcaac aacatataat ggttgttact cgcaaataat attaaagcct agtctccaat
      301 tctgattttg tagattccca aagagttgag agacggtcca ttatagacaa aaactctttt
      361 atttgatttt cttttaaaag agtttctgct tcgcgagcca cattacagaa atcttctaat
      421 ccaaaatttg cagcaactcc tttaatttga tggagaattg gttgtatgag tgaataatcc
      481 gaattagcca ttagagaatt aatttgcttt ctttttccat ctaaattttg aattaaatcg
      541 gcaatagaag ttttaagcca aagtaagtct tcctcacttg acttgtcaat aaaagcttct
      601 attcgaaccc aattcactag catatattca tctcaatatc tacagacact atattatttt
      661 caattcgaaa gaaaagtatt ttattttact caaattgtgt tgtaagagta atttaaaatc
      721 tattgattaa atttgcctac tcagttgatc cactctcttt aaaacatttg gaatccgata
      781 ctctccttct aaactctgaa ttaaatcaaa agcatagttt aaatcaatac caatcgaact
      841 gatatacagt ggtttcttgc tcacccctcg atatagttct ttatagctag aaacagatcg
      901 aaaataagtc tttgcaaccc caatcaccgg aatcttcttt tccaatgcct catataaata
      961 cccaccaagt cctttttttc cttcctcaag ccaaacgaag ctatccaaaa taataatatc
     1021 gatttcctct tctattaaat cgaatgttct aagaatacat ggcaattctc ttttataaaa
     1081 ctcacctggt atgtattccg aagtctcttc tattttttta ctatattcag atagaacttt
     1141 ttcttctttc tctttcttaa atacaacaca acagccattc gcgtaggctt caaaataata
     1201 tacgtcgaaa acagctatcg ttattggttt catttttgat ttaaaagtta aaccttaacg
     1261 cacaacgtta aagtatttta gaataatatc actatcgact cattccatat gtttagcgac
     1321 atatttgatt agcaaacaaa tttcgtaacc caacattaaa ccagtatttt actgtgtttt
     1381 ttacataaca ggataagatc aattagccac agaataactt tttttaaata ctttcaaaaa
     1441 aatattgcgg attctcggag tccttgtcga tgtgtatgat aggtgtatct ctaaattttt
     1501 aggaggcaaa tatgaatgta gcacttggaa ctttaatttc ttcactggac aatctagtta
     1561 aaaataagaa tgccgcgaac aaaaaaacaa gcgatgctgc acaaaatgca ggcgttgagt
     1621 cgccaatatt tagtgcatcc atacaagcta gataccaagt tttacaaagc cgactgagta
     1681 aattgcaaaa tgatctttct agagagcaag caaggcttgg aattctagaa aaagagaaca
     1741 ttcaaccaga agagatttcc aatattctat ttggtgcaac acctcttttt actgaatctc
     1801 tagatgaagt catccaagaa aagcaacagc tagtagagaa aatcaatgca caaaaaagat
     1861 ttttaaccga acaaattcga agtgtagaaa tccagtctga aaatattcac tctatcggac
     1921 ttggaaatgg agataatgta gaagagaaac ttggtatgct aaaaaaatcg caacctccag
     1981 ttatcaacat ttcccaaaaa agtgtagaaa gactcatcaa agattaaatc ttttatacaa
     2041 ataaaaagaa accccaaaaa agaatatgga ggttgaccac actgcgaccc atggtggaat
     2101 ccctccattt tcccctaatg acttacccgt tgaatacata atataatata aaaaaacaac
     2161 tccaacactt atacccagag acatgacacc agctgatttt gttgtgtatg cacctgcaat
     2221 agctccaata ataacaacaa tcaaacacat gataggaact gcaaagatcg catgacgttc
     2281 tacttctaag tcggcatagg gaacaccttt gttctttcgc acaacgattt cctctgacaa
     2341 ttcaaaaata ttcatctctt ccactttctt acgaggcttt gagaagtaat ctgcagattc
     2401 aggaaatgta taaatctttt ctccataacg agtatatgat ttgactttca tatgttcatc
     2461 aaagtatatc tcttcgatct tttccagttt ccaatttttc tctgactcat tataacgtgc
     2521 attttgtgct gaaataacat aatcggctag attcttctca ttgagcttta tataattaaa
     2581 cccaccacga acggatttag taggagcatc ataccaatag acatagtaga atccttcttt
     2641 ccctttaata tgtagttgat aaaccaagtc tgtctttgtt ccgacacctt tttgcacgag
     2701 agaatactca taagcagcca aactattaaa aggacgaaca actacttcgc ttaaaaataa
     2761 cataaagatc cacatgaatc ctccaaaaat aaaaattgga gttacgattc tgtaaaaaga
     2821 tacgcctgca gccatagatg caacaagttc tttatttgtg ctaaattgcc ccacaacaaa
     2881 gcaaacagag aacataagag acatgggaat cacaagcgtc gccattttag gaagattata
     2941 aaagagaaaa agatagatgt gaacggaaga ttctttagat gcaataaagg tcttcatatt
     3001 gtccataact tgggaaacaa ttaaaattcc cacaagcatg attaaggttc caatgaatgt
     3061 attaaaaaat tctaagaata agtatttatc taaaattcga atagggaaaa attcagattt
     3121 aattctttct atataagcaa tcacaattac aaggaactag aatttaaact aaagtctatt
     3181 ccttttttat aaattgaatt gccatcatat caccctgatc tgtccatttt acaaagcgat
     3241 ctaaaaattt cttagcaact ttataaaaaa tcgaggcatc ttttttagct gtaagaccgc
     3301 ttccgtaggt tatatgagaa atttttttaa atccaatctt ttcgcattca gaaataagac
     3361 ctttaaggga ataataataa agatgctcag gaacgtttaa atacctccat cgaatcccag
     3421 tcagacgtgc aaggattcca tatctacaag tggataatat cattctacca ccgggtttaa
     3481 tatgagaaaa tattttctcc aatgtctctt tgggtttatg caaatgctcg atggaagccc
     3541 aaagagtaat caaatcaaat ttttcgtttc gttcgatatc ccaattcaaa aaatcatctt
     3601 ttgtaatgga aagttttaaa acttcttttg caaagcgaac aggaccttct gcgatctcaa
     3661 tcccttctgc ttgccagttt aaacttttca tataattaac aaaatagcca gcggcacaac
     3721 cgatatcgat agttctctta gaactaggaa gactcttttc ccattcaaag aaacctaaat
     3781 cttccagatt taatcgaaag accctttcaa tttcagttct aactttttcg gaataataat
     3841 tatcgtaacc cctatctgtt cgttttgtaa aatattcatc tgaataatat tttgccactt
     3901 cctcaaaagt aggttgagga tttacctgta tgagcccaca atttttacat tttacaattt
     3961 ggaatatttc cttttgactg ctttcttttt cgaaaatagt tttaaaagat tcattttgac
     4021 aggaattaca gggaattaat ttcatattaa taatttacct tacacacaag aatgttaatg
     4081 cgatagacgg gatactctct aggttttgcc aactacaaga gctcggttca cactcaaata
     4141 gctcttgttc ctacctgaat ttttgttacc agtcgttgaa ttgatctatt agaaaaatgc
     4201 gatgcaatca tttcttttat catacttaat aaatcctatt tctctgtatt gccaattagt
     4261 aaatagaacg aacttattta ttcttataca tttaaaaaaa agaatctttt tcgctttatt
     4321 ttcatcacca atacactttt ttcatttgaa aagccaaatg caaaagaagc taacaaaaat
     4381 tagccacata ttcgcagatt caatctattt ttgacaatag tgtttattct gctaaaaacc
     4441 aagattcaaa ttctttttga aatttaacag ggatgaattc agtaaaatga taatcggcaa
     4501 aattattttt tgcaaacggg tctttactgc aaaattctct tatcgcttct aggctttccg
     4561 ctctcgcaat taaaattccg ccatccctag gattttgtgg accagaacta agtaatattt
     4621 ttttttcgta tccagaatct aaaaacgctc gatgctcaac caaaactgta tcaattttct
     4681 cgataggaac tttataatct aatcttataa taaaatgttt catacatatt tcaagatttt
     4741 tgattctaag ttcaaaacaa gtgaaaaaaa atatttaaac tattttagat aaatcaagaa
     4801 ggaaattttg aatttacttt cttgctattt ttaaagaaag atttccaccc agtcctcctg
     4861 acacactcaa caaattctta tcagaatatc gaaaacttgg tattttataa caatctccaa
     4921 atcattttgt ctaatctttt gaataaaaaa aacggcattc atcaatccaa actctactgt
     4981 ctttgataaa agctctagtt cctacacatt gttcacacct ttgtagatct aagattaaga
     5041 tatcactaaa cagaaatgtc actacgtaga aaaccagctg ctcaaaattt ccaaacctca
     5101 taaatacgat gtgcttatac ccccaacccc tatttttagt aatttattag cattcgctaa
     5161 tattcttttt tcatttctat atgaatcgaa aacttccttc tatccaatgt gaatcattct
     5221 aagaaacttg aattatgact gataagaaac tcctcgcaaa caaataagcc ttaattggag
     5281 aaagataaaa aacttgtata taaaacatga aaagtcaatt tggttaagaa taaagggaaa
     5341 acagaatgaa attagcagat tattttaaaa tcagtttaaa aaaaccagaa ataacagaat
     5401 ccattgcaca ggttttagat aaaaaaaata aattaaaatc tcaaattgaa acttgctttt
     5461 ctcgtttaga atccatagag atttgtaaca gtaactccaa gtcagccgat gcaatgatac
     5521 tctcaaatta tttaattgta gatcttacta atttaattct tatatattta aatcaaccaa
     5581 cgcttctcag ctctgataaa tggcaagaca aaacgaaaac atttccagaa gaattaagtt
     5641 cgattttttc tgagcacaat tccattttaa attcttccta tgaaactgat gaagaaagaa
     5701 gtgaaaaaat tgaatcttct ctctctgaca tacttcttgc ctcggaaaaa tttatattca
     5761 aaaagaacaa agctgatttt caaaatccaa tcgatgatta taaaaagaga tctgccgttc
     5821 agtttatcct aagtgtaatc attttgattg ttgctatcaa tacaggtatt aaactattta
     5881 ataaatacaa agcagtaaaa gaagacaccg ctaaaatcta tttcatgaac acagcaaatc
     5941 ctgctccaaa tccaaataat actattactg ctaaagttat cccatcggaa aattgggtag
     6001 aggctaattt tgttttgccg aatcctgtag atataaagga aatcaaagtt gaattaattc
     6061 accaagtatt tacaagattc caactaaaag atttaaagtt tttagatgag aataaaaagg
     6121 ttctccgaga gagaaatttt caattaaaca atttaggaat ggtggaaaac tcagaaaaaa
     6181 atgaaatctg ttgcactgaa gaattaaaac caggaaagtt aatcccaggc aaatatatgg
     6241 aactagaaac aaccagttca tccccttctt tctatgtaaa aatggaagaa tctaaatctg
     6301 taaaagaaat agttctaacc tatcgttaca taaaaaatac aaacaaattt cctgattaga
     6361 taactcaacg gattttatct tagcatgttc aaacaataca taatcctgct acgaatccac
     6421 caatggataa aaaatacgat tttatttgct ggggtaatat tcggacataa attatctgat
     6481 ccagaagcaa ttctaaaaag cttctttgct tttttattat tttctgtgat tgcaagttgt
     6541 caatatgtga tcaatgacta tctagataga aaagaagatt cactacatcc tgaaaaaaaa
     6601 cataggccac ttgcatctgg caaaataaat ccaagtttgg cacttttaat aactgcaatt
     6661 atacttcctc tctcacttac tctatgctac atgttacatc cttatttctt tggcttatgt
     6721 agtttctact ttggattcaa cctagtctat agtaaatatt tgaaacatat tgtaatatta
     6781 gacgttatga gtatcagcat tggttttgtt gtgcgagcaa tctctggagc tgttgtaata
     6841 aacgttagct tctcatcttg gctattactt tgcacattta tgcttgccct cttttgggga
     6901 tttagcaagc gaagagggga attaatttta ctcgaaaaat cagcttcatc tcaccgaaaa
     6961 attctagaag agtattctcc tcagttttta gatctaatga tggcaattgc ttctacaatg
     7021 acgttaatca gttatgtgat gtatacgatt agcccagata cagttcaaaa gatgggaacc
     7081 gataaattgg tatatacaat tcccattgtt gtctatgcaa tttttagaag tctatacatt
     7141 atttatataa aaaatatggg acacaatcca agcaaggcaa tcttaaccga tctaagtgtt
     7201 ctagcatctg gactcatctg ggtagttatg gttacaatta ttctctattt caagattcca
     7261 agcgtataat gaaaagtatc cgcaaaataa ttgaatatgc tttttatata atcatctttc
     7321 tagctataac tttttcgatt gccttttttc gaagcggtag tcgaaagcta atattggatt
     7381 tatctaaata caaaccaatt aatacagaat tagcaaagga ttttgaatca aaaaacaaag
     7441 tgatttactt ttgggcaact tggtgtgggg tctgtgaaac aaacctgcct atgttcaaaa
     7501 caagctatcg tttattcaat ggcaaatgga atacagaatt tatttcgatc gaagaaggct
     7561 catccactcc tgaatatgta aaaaaatatt tagataaaaa tgaaatcttg tatccaacaa
     7621 tcattgcaga ctcgaatctt ctacaatcaa atcagattag tggttatcca accattttct
     7681 ttgtaaactc tcaaaatgaa gtaaaactca ttgataccgg gttaatgagt ccagtcagta
     7741 tgtttatacg tatcttattc ttaagtttag gttaaaaaat gttttcaatt ccagaattaa
     7801 aaaaattaaa aaaagagcta ctacatagtt taataaaaga agcaaatcaa tatttgccac
     7861 agattccaaa aaaatacaaa aaagattctg aaaaagtgat aaaagcttat attgactctg
     7921 aatctatcgc tgggctagaa atttgggtca attcgattgc tgagttttta tattttgaag
     7981 aagcaaagag ggatttaaaa gaaattcgaa acaatgaagc tgacctcaaa gaattccata
     8041 aagcctttat aaatagtctg aaacaagact ttggagttga agattttaga ttcatcgatt
     8101 gggagcgagc taaaccaaat tataaaaatc tctcaaacct gttcgagaat ttttctgaaa
     8161 tagcattgaa gccatgctat actatagacc ataacgttta tttaaaagaa gaatcaaatt
     8221 attcctacta tatgagagcg aatggagatc cttcgcaaaa gggtgtcaaa ataaaaatga
     8281 cacgaacaaa aactcctaaa gaaaaaatgg atcaaataca aaaagctcat gaatatctag
     8341 aaacattttg ggaggaagga tttcaactct atcgcttact aaccgacaaa gttcatatag
     8401 ttcaatccaa tggacttgtt agttacagtc attttcatga acaaggcata tcttatatca
     8461 attttataga tagagatata ctagagtcca ttgatgattt aattcatgaa aattcgcacc
     8521 accacttaaa tcttattcta aaaaaatata aaatagttaa aaaagaattc aaagacgata
     8581 ttttttattc tccttggaga aaaacacttc gacctctcta tgggatacta catgcaactt
     8641 tcactttttc ctatgcagct cttttgtttt acaatatcgc aaaatcaagt cagtggactt
     8701 ttttagacat tgatcaaaaa ttcttagaaa aagcatattt tcgatttatt gaagaaactt
     8761 tgtatgtaga atattctctt tatgatctac gttttgctat cggcaaagac ctgtttaccg
     8821 ctaaaggaat tgaattaata gaaagtttag ataaatacaa tagagattgt ttactctact
     8881 tgcccgatgt tctaaaaaaa ataaaatcaa aagaattaaa aaaagaaata aaaaatattc
     8941 agaaagtttt acataagaat cggactcaat ataaaataaa tagcacacaa caactatcaa
     9001 aataaaagag actctcaaag attactaact aacacagcaa acgagtggat ataggtggac
     9061 aatgaaaggc tctacgaggt tcaccacatc actccgataa gaaatgcgat aattcaaatc
     9121 tctttatctt tgagtgattg gtttgcaacg ttgcaaatca tttcatttct tccttatctt
     9181 aaaaactatc caattataaa aaaataaaaa atttacaaac taagtcttat ttaaatatag
     9241 tctaattctt acaaaaccaa ggggacgatt tggtttcgac tgttgtagac ctgggtattg
     9301 tggcatgtag aggtgagaga ccgcctttaa aaactctcaa aaaacaaacg ctaacaacga
     9361 attagcttta gcagcttaat ttaagctact acgagaaact gctttaccct tccggtgggc
     9421 agcgtatcga cactaaacgg aaggttgcac aagtcgttct ccaacttgga tttgtgcaga
     9481 accaaagttg gataggagtg attcccctgt ttgtaggtta aggcacttcg agatttattt
     9541 acaaactaaa catgtagagg ctttacttat gtgcaatagg acccgggttc gacccccggc
     9601 gtctccaatt tggttcaaaa agccttaaag gcttccgatc gtaatgggtc attctagttt
     9661 gtatctttat gatcgcaaaa ccgattattt taaatcagga tttctttttc taagttcttc
     9721 atccaatttc ttttcaaata aaataatatc taaagacata aatccaggaa agggaagttt
     9781 aattatattc ttttgcattt gcataacgtc tttcaaaaaa ccaatcacct ctcctatccc
     9841 taaatcccct tttttatatt tttctatttt ttcggcaaac tcagaattta aaatataaat
     9901 ctcctgccca ttaaacaaat catttcggag catattaaaa gtatactgag aatattgaat
     9961 gtcttttata aacatctctg ctagcaattt acgtttgtct ttttttacac cgaaaaaact
    10021 tactttttca agaccgattg caagctcata ttcactataa ccaaaaatag atttataaag
    10081 cccaaataaa actgcgatcc caaaaataga tataaacacc gctacgacat atcgaccaat
    10141 agaaccttct tcatttgtga ttgaaacaat gattccaaag gtaaacaaga aagaaataac
    10201 agctaacaaa ccaataactc gatatagcat actaacgttt tctttccatt tgaataaact
    10261 accattcgat gtttctgaaa acaaaaaagc tttggacttt ttctcttcgt cgagcaaatt
    10321 atagtcttct gcactatcta gttttattat atcttgaagt aggtttaatt tctttcgagc
    10381 agctagctcg gtatcaaagt gaacaatatc ccaccaagcc ccatcttttt ttataaaaaa
    10441 tacaacataa taagtagttg aggattcgct tccacttaca acttctcttc gcatatcgaa
    10501 agaagaaatt tctccaaagg gaatataacc actttctccc gacttagtaa gcatataaaa
    10561 tcgattactc tctagatcaa aataagcact cactattttt ctctttttac ttatcattga
    10621 atataaataa gcagtggata taaataaaaa aatagtgata ccagggatta taaattttaa
    10681 tacatctaac aaattgcctt ctgttcctat acttgccaat ccaaggaaaa caagtattag
    10741 ccccacaata aaaaagataa aaccgattat gcttaagata atttttacta taactgaatc
    10801 tatagtattg tattgaaatg ataattcctt ttccattaac gtttctccgt cagttcttat
    10861 attcactcat ttatagattc ttattgcatt tttaaacaga aaatcacagg taaaaaaata
    10921 actttgaatc acaataaaac gaaaacagat taattctgaa attatatttg tcatccttat
    10981 tttttactaa cggatttcta tgagatcaaa aggtctaagt ccaacatcaa tactcgttgc
    11041 agttaaacga ggtttatctg gttttgtagt atcaaaaata agaacacctg gtttagaaaa
    11101 acttccgtct cccatataca attttcccga actagaaagt agtaatccag aaatatttcc
    11161 agcactcgcc gggaaattga gaatagtttt aattctttgt ccagtcattg gattaaactg
    11221 gaagataaat ttattaaaac tagaatctaa aacagaagca tagccttcat tttcatcttt
    11281 gatctgcatt gcaagtatgt cccctcctgc ctctgtttcc gaaaataaga aattgcttct
    11341 aaatgttttt gtcactaaac taaaagcaac aataccgcca tctaacttgc taataaatcc
    11401 cagcctattt ggagcacaca ttgcaagaaa agcttctcca ccaattgtaa ttttttgaat
    11461 acgactagtt ggatttctag atttaaaagt ataagctcga ttgactagat ccgtattcac
    11521 atccaactca agaagaagag aatcactgtt aggtgatggt attccagttg gatcatttcg
    11581 atctaatctc tgcaattgaa tgtatagtaa atttccttcc aaaaacattt ggctcatttc
    11641 aggaattcca tccgatccag ctgcgccaag tgacgtctgt tcagcataaa taccaagatc
    11701 aatatttcgg ataaaggctc cagagtaagg atttacaact aataaatttc tactattata
    11761 aagtgtgata taagccttat tctcattgac cttaataata tcttgcgggt tatttccctt
    11821 gcccacagaa tattctgatt ctggtaaaaa tccatttgct ggatttaaga tcagaatact
    11881 atcacggttt aacctattga ttacaaatat ttttccatct atcattttcg caactgcgtc
    11941 cgagtgaatt gttgcgatag tagtaatact taattgtgtg tccggattta taacagcaaa
    12001 ttttccactt gttccgaaat cactggtaac tacacctacc ctttctactc ctgcagatgg
    12061 aattacaagt agactattaa taaataaatt agaaagaggg ggacgcttga tatcatcaca
    12121 aaccaaaaat aaagtcgaga tcaaaaatgg aaaagctcgc tttattctag ttagaattgc
    12181 attcatttca aaaaatcaaa aactctgctg aatataattg aatatatctt gaatgagcat
    12241 agtctctcct gaactcagag gaatcggaac aaaaatgaga acaaaggagg ctaacataaa
    12301 atagcctaaa aactttcgac cgagtcctaa ttctgtagag gagtctggca catacggatg
    12361 ttcaatcttg ataaaataat aaataataaa accccatatg acccatgtaa aactcacaag
    12421 acctaaaagt aagaaaaata aaaaaagata atgaatccaa cttctatatt tttctccaaa
    12481 caaagcatat actatgtgtc caccatctaa ctgaccaaat ggaattagat taattgctgt
    12541 aattaataat ccgacccatc ctgccttcgc caatggatgt attaaaatgg ttgtattcac
    12601 aggatcgtag ggtcctagaa tccattgacc tgtgtaatag gtaaagagac tatccccaaa
    12661 atttaaaata ttcttttgat tggcaaattc tgctagatcc acaagattcg aataataaag
    12721 ccctattacc caacatatag cagataaaac taaactagct acaggacctc ccacaccaat
    12781 atcaaagagt ttgattttat cttgaattgg ttcttggatt tgaatcacag cacccatcgt
    12841 tccaacagga cttgctggca tcggtataaa ataaggtaaa gttgttttta gattataaat
    12901 tcgagcaggg atataatgac ccatttcatg acaaaataag atcacaagca aggatactgc
    12961 ataaggatac tcagtccaga aaatcttagt tagtatttcc ttattttcct caaaggcaaa
    13021 caaaatagcc cacacacctt ctttgaaggt tagtgttaaa aacgtaagaa caattaataa
    13081 aatatgaaat ccgactttac ttttcactcc aactaatctt aattgcatag agggttcgtc
    13141 aagaccctaa attgtttttt aataaaaata atatcaggtt acgataataa gtatatgctc
    13201 aatcttaaag atctaagtat caaatccaag ctcattcttt ccgtgggaat tttccaagtt
    13261 ctatcactaa tcattgccat tcttggcact atgggattat cccgtatgga aaaagaactt
    13321 ttgattgttt cggatatttt tgtaaatatc gaggtcaatt taaatgatct caaatttacc
    13381 gtagatagca gaagacttgc cctgctacgt gcgttaaaca cagattcgca ggcagaattt
    13441 gataaagaaa tgactttagt taaaaaatac gaatccgaat tcaaagacaa tatcccaaaa
    13501 atcagaaaat tcctccaaga aaatgcaagc cgctttgaaa caatcaagaa aaagccagaa
    13561 gactctgatt taaaagattt ttttaattcc ttagatgagg tagagaaatt acttgagacc
    13621 tttgcaagcg ctacacttat ttatgagcaa aaaatgtccg aagcatttcg atatgatact
    13681 ccggattgga gaaagttaca gcttaaaaaa gaaagtatgg agatgtattc ggaaagatct
    13741 ttgaatcaca caaaatttat tgataagata aaacaactaa atcaattcac tgctaatggc
    13801 cttggacaaa gtactcaagg tgtcattgga acatcctatc agctaaaatt ggtactctgg
    13861 attggatatg ctatatcttt agttattgta gtaatcgtaa cttacatgat gattcgtaga
    13921 attgcatatc ctattatctc tgcagaaaaa atggcaaatc gatttgcaga tggggatcta
    13981 actatctaca ttgaaaacaa ttcgaatgat gaaatcggta gactcgtcag aggcttaaat
    14041 aaagcatccg agaatttgaa gaatgttgtt aaacaaattg gaaatacatc agatcattta
    14101 gcatcatcag cggaagaatt atccgcaaca tctagaaatt tagcggatgg agcaaccaac
    14161 caagcttctt ctctagaaga aactgcttct gctgtaactg aaatcacaga atccatttct
    14221 tatgtttccg aaagtgcccg tgaccaagag aaagaagtga tcgaaaccaa taaatcgatg
    14281 aatgaacttt ctaagtccat tattcaaatt acagaaactg cacgtgctgt aaatgagggt
    14341 tcacagtctg cccttgcgga ggcgaaagaa ggacaagtaa aagtcaatga aactgtaaaa
    14401 agaatggcag caatcgaaga aagttcggaa caaatctcgg atattatcaa tgtgatcaat
    14461 gatatttcag agcaaacaaa tcttcttgct ttaaatgctg cgatagaagc ggctagagcc
    14521 ggagagtctg gtagaggatt tgcggttgta gctgaggaaa tttctaaact ggcagcacgt
    14581 tcgcaaaagg cgaccagaga gattgcagag ttaattacgg atagtattac caaagtaaat
    14641 gatggaaaat taattgtggg acaagtggtt gaatcgctga ataagatttt acacaaatca
    14701 atggaagctg cccaactttc ggactccatt tcaaatgcta ctaaactcca aagttctgga
    14761 aaccaaaaag ttttaaaatc tgtcacaact ttatcgcaaa tggctcagtc catttctcgt
    14821 gcaacaaacg aaatgaagat ttcctcaaaa gagatttcga atgcaatcga gcaagtaaat
    14881 tcggttgcac agaatacagc ttcctcatcg gaagaaatgg ctgcttctac ttccgagtta
    14941 gctgcacagt cagaaaaatt aagtcagttg attggtgtat tcaaaacgaa ctaatcgaac
    15001 ctttctctga gagattgtat aaaatttaaa tttccaagtg actctattaa aaaagggttc
    15061 acttggaaaa ctttctataa aaacaccttc ctcaccctaa aatgatttat acattaaaaa
    15121 aacaatcaaa tgattctttt gcaagagcag gtgaattgaa tctgaatggg atcaaaattg
    15181 aaactcctgt ctttatgcct gtaggaactc gtgggacaat caaagcgatc acttcagagg
    15241 atattagaga actaggtctg gaactgatct tagggaacac ttaccatctc tatctaagac
    15301 ctggaacaga ggtcttggat tcttttggtg gtctaaaaaa attcatgtcc tatgaaaaag
    15361 cgttattaac tgatagcggt ggatatcaga tattcagctt aaatggactt tttaagtatc
    15421 ataaagatgg agttaaattt agttctcata tagatggttc caaacatgaa ttcactcctg
    15481 aaaaagtaat cgatattcaa agatccattg gatctgatat tatgatggtt ctagatgatt
    15541 gtcctcccta tggagcagat atagaaagat tggaagaggc tcttacaaga actcattact
    15601 gggcagaaaa atcaattcgt cactttgaaa aaaatagaaa caatcaaaat ctatttggga
    15661 ttagtcaagg cggagtcaat ctagacctta gaataaaatc actcgaattc attcaatcgc
    15721 ttccatttga tgggattgca gttggcggtt tgtctgtagg agaacctagg ccagaatttg
    15781 ttcgaattct agaaggtatt tcatccaaat ttgatcccca aagacctcat tatttaatgg
    15841 gtgtcggaac tgttacagac atattagagg gagttaagaa tggaatcgat atgtttgatt
    15901 gtgttctccc aacaagaaac gctcgaaatg ggcaagtatt cacatcatat ggaaaagtaa
    15961 atttaagaaa tgaagcacat cgtatgtcgg atagtcctat tgatccaaaa tgcaattgta
    16021 aagtttgtaa aaattatagt attggctaca ttcgacatct acataaggta aaagaattgt
    16081 tagctttttc tttgagcaca tatcataatt tattttttat gaaagaattt atgaatggaa
    16141 ttcgacaatc tatttataat tcaaattttt tagaatatta tttatattgg aaaaaattat
    16201 acgattaatc taaattttat cgttgactta ttttattata gtggatttat atattagttg
    16261 ccaaaaatac ctcaaactga ttattaataa ccaaggaatg gattaacaag aatggaaatc
    16321 acaagaagag agaacaaaaa catcataatt cttgacatta atggtgaaat cgatttatac
    16381 aatgcaccag agattaagga tatcattgct aaattgattg aagagaagcg ttatcaaatt
    16441 gtcatcaatt tagaaaaagt atcttacatt gactcttcgg gaattggtgc tcttatttca
    16501 agtttatcaa acttaaagaa ataccagggt ggactcaaaa tcattaacgt tgctggttct
    16561 gtgagaaaag ttttcgaatt aacaaaactt acctctttct ttgaaatctt tgatgctgag
    16621 gcagatgcta ttggcgcttt taaataatca aatgattatt taaataaagg catgagagag
    16681 aactcctcaa acattaaaat ttttttctta ccttatttag ttttctgtgt tgtttcttgc
    16741 ggagctaccc ttcctctgca agaaatcact acagcgaaac aagaaattag caaagctaag
    16801 ctttttagcg cagataaata ttccaaaaaa gaatacgatg agtccaagac aaatctctat
    16861 gaagctcatg agagcgttag tagtgataaa cccgatctaa aaaaagcaag ccagcttgct
    16921 gaaaactctg ccgagaaagc aagacttgcc atacagaaat cacttcctga atacataaaa
    16981 gacttacgaa aagaaagtat atcctcctta gatgcagcaa atcaagcgtt agccgactca
    17041 ttagcagcgg acctttatga acaagctaac cttctcaata ttgaagcaga tgaactcaca
    17101 aaaaatgctg actctagact taagaataca aataattcat cagtaaacag caatgtttat
    17161 gatgattatg agaaaggaat caaaagatat aaagaatcta tttcgctttc agagaaagca
    17221 agagatctaa gcttggcaca aacacaggcg attattgatt caactacaga tattgaaaca
    17281 aatttagact tggtagaaaa atatgcaaag aatgataaat ctgttttaag taatgttaaa
    17341 aatttaagag gcgaatacaa atcttctatc gatacaatgg aatcaggtag actcagagaa
    17401 ggcttcaaaa aaacagaaga aatccgcaat agatcaaatg aactgattgc atccgtaatc
    17461 ttaccttatg caaaagatag aattaaacta gcaacaacca agatagaaga agcagagaaa
    17521 aatattaact ctgaagaaaa agaagaaaac tctccctcga taaataagga caatctatct
    17581 gccgccaaag aggcatttaa ttcctctgtt gatttacttg gaaaagaaag atactatgat
    17641 tcaatccaac aaagtgatga gtctattcga ctggcagaag aaattttgaa atcgaaaaca
    17701 aatacactcg caaataaatc tcgcgcaacc gaggagaaga gtgataaagc gatagaggat
    17761 gcagacgcaa gagactcatt tccaagaaaa aacaaagcaa agactactga ccctaaagca
    17821 gatatcaaag atggctggaa gaaatacatt gtaaaaaaga aagttccccc tgatacactt
    17881 tggagaattg cagcaaataa agaatatcta gggaacaagg ataagtggaa ggaaatctac
    17941 aaagccaata gtaaaacgat ttcaaatccc aataaaattt atccgaatca ggtaattcta
    18001 attcctccaa tccagaaaaa gaaaaaatag ttttttattt tttacttaaa caccatatat
    18061 aaaaaataag aatctcttct aatccgcaag gatactgtaa ataataagca aggaatgatt
    18121 cgctacttta tacgcatgta tgacttaact gttctctaag gtaaaggtcc ttcttatatt
    18181 aaaaagactc taaccatttt gaaaaaagtc caaacaagga acaggattga aataaaaatg
    18241 atgaaatact tttgtgaatc ggttaaaata cttccatcca atcatttagg aataaagttt
    18301 gatttatgga tagattttat actacacctg tagaaaaatt agaagcaaca gattttaaaa
    18361 atttagttcg cctggctctt gaagaagaca aaccagaaca ggatattact tcagaagcaa
    18421 tttttgaaaa agatcaaatg gcgactgcaa ctttaattgc aaaagaatcg ggaatactcg
    18481 caggcagcat tgtattagaa gaattaaaag accaaacagg tcatctgttt tcttacgaaa
    18541 cattcaaaaa agatggagag gagtttaaac caaaagataa aatactccaa ataaaaggca
    18601 atttaattgt aatcctacgc attgaaagaa tactcttaaa cttcttgcaa tacctttcag
    18661 gaatcgcaac agaagcaaat aagatttcaa gcaaatataa aaataggtta atgatatttg
    18721 atacaagaaa gactcttccc gcctatcgca aattagcaaa gtatgcagtc tatattggag
    18781 gagcttgtaa tcatagacaa aatctttccg acatgggtct aatcaaagat aatcatgtcg
    18841 caatgtctgg ctcaattgaa gaggctgtaa aaaaaataag aacaaaattt cccaacaaga
    18901 agattgaatt agaaattgat ggattgtttc agttagatga tgcaatcaat tcttctccag
    18961 atataattat gctagataat tttactgttg aagatacaaa aatagcagtt gagaaaattt
    19021 taaaaacaaa gcctcagatt cgaattgaat gttctggggg tattactcct gaaaaactca
    19081 attcactttc agaaattgga tctatcggtg taagcatggg atatttaaca catacaactc
    19141 gctttttaga tttaagttta gagatagagc aaaactaatg ggttttttaa aaaaagaagt
    19201 ttccgtagaa gatttatttg aagcagtaaa ggaaagattc agtccagaga atctagattt
    19261 aattaaaaga gcgtatgacg tttcgaatag aacacacgct gaccaaaaaa gatattcagg
    19321 ggaaccttat gtaatccacc ctgtaaacgt tgcgtatata ttgaatgact taaaacaaga
    19381 gcctgctgta gtagctgctg gactattaca tgatgtaatc gaagatacaa gttacacacg
    19441 cgaggatatg actaacgact ttggcaaaga cattactaaa cttgttgaag gtgtaacaaa
    19501 aatgtccaaa gtcaaaaata aaactagaat gaattttgag tctattgcag cggaaaattt
    19561 aagaagaatg ctaatggcta cagcaaaaga tcctcgcata atcgtaataa aactagctga
    19621 caaaactcac aatatgcgaa ctcttaaatt tcataaaccg gaaaaacaga aaatcatcgc
    19681 acaagaaaca ctttcgatct atgcgccgtt agctggaaga cttggagttt ataaaataaa
    19741 atcagaatta gaagatcttg cctttcaaat tttagagcct gagaaatatc aagagataaa
    19801 agaaaaagta gcatctaaaa aagcagaacg tgatagcttc attgaaacag cgaagattat
    19861 catcaaacaa agattagctg agatcaactt agatgttcaa attgaaggaa gatcaaaaca
    19921 cttctattct atttataaaa aaatgaaaga caaagataaa acctttgatg aaataaatga
    19981 ccttagagcg attcgaatta taacaaaaga agttagagat tgttacgggg tcttcggtgt
    20041 agtgcatagc atttggactc ccgttcctgg gaaattcaaa gactacatag caactccaaa
    20101 aacaaatcaa taccaatcct tgcatactgc tgtaattgga caggatggaa gaccttttga
    20161 aattcagatc cgaacagcgg aaatgaatca tatcgccgaa catggaatag cggcacactg
    20221 ggcttacaaa gaaggaacca aatcctctaa agatgttgca atgacagcaa agtggatcga
    20281 aaagattcga gcttggagcg aatctagttc tgactcgaaa gagtttctag aagacctaac
    20341 gcatgaatta catgaagatg aagtatttgt ttttacaccg aaaggcgata tttataactt
    20401 tccaaagggc tcaacgatac tcgattatgc ttttagaatt catacagatg tgggaattca
    20461 tgcaaaaggt gcaaagattg gaggaagaat ggttcctctc agaacagaat taaagtcagg
    20521 tgatcaaatt gaagtaatta cagacaaaaa agtaaaaccg tctcctattt ggcttcgaat
    20581 tgtaaaaacg tcatccgcaa aacaaaagct tagacaatac ttccgtaagg ttcaagatga
    20641 aacagcgagt gagacagcta acgcatatgc agcaaagaca gttgtggcgg atcaaccttt
    20701 gcccgaaatt gatttaaccc aactaaaaaa gctaaaacct gataaaaaga aaaataaaga
    20761 aaaaagagag atcagtattg tagtagcagg cataaaagat atactcgttc gactctcttc
    20821 ttgttgttct cctttacctg gcgatgaaat tgtggggttt attactcgtg gtcgaggagt
    20881 cagtgttcat aaagcaaatt gtgatgtagc acttcattat tcagataaaa aaagaattgt
    20941 agctgtcaga tgggatggag tgaacaaccc tgttccggtt cgcatagagg taaaagcata
    21001 cgataggcag gggatttatt tagaaatggt tgagtctatt tccaaaacag aaactaatat
    21061 tttggaagca ggcgccagct catcaggtga aagcacctta ctagctcgat ttttaattga
    21121 aatagaacat ctagaccagc tccaggaaat attagataat atccgatcta ttaagaatgt
    21181 tttatatgct gaaagagtaa tggaaaaata atttttcaaa ataaccttga cgcatttaca
    21241 tattttgaaa caggattgga tattccatta gaggattttt tatgaaagga attatacttg
    21301 ccggtggttc aggaacaaga ctctatcctg taactcgtgt tgtcagtaaa caattgatgc
    21361 ctatttacga taagccaatg atttactatc cattatccac attaatgctt gcaggcattc
    21421 gcgaaatact gattatatca accccaaacg atagcccact ctttagagca cttttaggtg
    21481 atggctctga ttttggaatc aaaatttcgt atgaaattca accatctcca gatggcttgg
    21541 cacaagcatt tatcattggt gaaaaattta tcaatgggga taacagctgt ttaattcttg
    21601 gtgataatat tttctatgga catggcatga ttgagaattt agtagatgca acaaaacttg
    21661 aaacaggtgc tgtgatcttt ggttattatg taaatgaccc agagcgttat ggagttgtag
    21721 aatttgataa aacaggaaaa gttttgggaa tcgaagagaa accacaaaaa ccaaaatcaa
    21781 attattctat ccctggatta tatttttatg ataagcatgt atgtgagtat gctagaaatt
    21841 taaaaccatc ccctcgtggt gaattagaga ttactgattt aaacaaagtt tatttacaga
    21901 aggggcaact cactgttaac ctcctaggga gagggattgc ttggtttgat acaggaacac
    21961 acaactcact tttggaagca tcacaattta ttgaagtaat tgaaaacaga caaggcttaa
    22021 aagttggttg cttagaggaa attgcatttc gaaaaaagta tataaccaaa gaacatctgt
    22081 taaaattagc agaaccaatg aaaaaaaatg gatatggaca atatttaata aggctagcca
    22141 atgaacattc aatcgactga atttgatgga ctttatatta tagaacctaa agtttttaaa
    22201 gacgatagag gtttctttta cgaaagctat tcggaaaaac attttgctga taaaggctta
    22261 aaattcaatt ttgttcaaga caatcatgca aaatcacagg atccaggcgt tatacgcgga
    22321 ctgcattatc aaaaacctcc ttttgatcaa gcaaaattgg ttcgagtaac aaagggtgca
    22381 gtgtatgacg ttgttgtaga tttacgtcaa agctcaaaga cttataaaaa atggtttgga
    22441 atagaactca gcggaggaaa tttcaaacag cttctagtcc ctcgtggctt tgcacatgga
    22501 tatttagtct taaaacctga tacagagttt ttatataaag tggataatat ctatagcaaa
    22561 gaaagcgaag gcggtattct ttggtctgat ccagatttac aaatcgattg gatggtaaat
    22621 aaaccaattc tttcagaaaa ggatacaaag cttccacttc tttcagaatc tcttggaatt
    22681 tttccttagc taacgatagg cgacagtcga atttcaagtt gcaaaggtgg attaaaacca
    22741 tttaggatgc atcatgcata agtcgagaga aattggtctt agcacaagcg atagcgattg
    22801 agccgtggta aagaaatttt atctgtttga aatttcttta ccgaagggga aagcgcggtc
    22861 ggaatctccc ctcttttgca aatataacgt tacccacaag tatgttaaaa ctgactacca
    22921 ccgataaaga cgatggacac cgatagttag tatatataat tagctttgcc attttacatt
    22981 atttgtcgct ttgatcacgt ttaagcatat aaagattcaa atattcgtcc tttaaaggca
    23041 tttaaccgaa gtaattatat cggtatatga atccatcgtt tttatcgttc tgctatcggt
    23101 gtcatcggtg gtaatttgtt aaaattgtgg gtaaggatac gttttgcaaa gagggggcgg
    23161 gggagatttc gaatcgccca gaaaatcagc ctttctcgag ataattttac aatagaaata
    23221 aaaggcattt aaaaaaaaat gacaggtgta tgaaagaaga taagaaacag gaaaacccat
    23281 attcaaaaac agttatactg ccgactacag aatttccaat gaaggcagat ctatccaaga
    23341 gagaaccaca aaccatctcc aaatggaaaa aagaaaaggt ttttacgcaa atgaggaatc
    23401 tgagaaaatc agaaaaaact tttgtgttgc atgatggtcc tccttatgcc aacggaaatt
    23461 tccacgtagg acatgctctc aataaaattc taaaagatat tatcgttaaa tccaaacacc
    23521 tagccggctt tcatacagat attattccgg gctgggattg ccatggtctt ccgattgaag
    23581 tgcaagtttt aaaaaattta ggcaaagatg cggctaatac gagttcaacg gatctcagaa
    23641 aaaaatgcag agactatgca aatgaattcg ttggaaagca aggcgaagac ctaagtcgct
    23701 ttctctgctt ctttgatgaa gatagaaagt atcttactat gagtccagaa tatgaagcaa
    23761 agatcataga agtattcgga gatttattta gcaagggtta tgtttataaa ggtaagaagc
    23821 cagtctattg gtgtatcgca ttaggcaccg cacatgcgga agcagaaatt gaatatgcaa
    23881 accatacctc tccttctatc tatgtaaaat ttccagtgat tggagaatcg gatacaagtt
    23941 gtctgatctg gacaactact ccttggactc ttccggctaa cttagcaatt tgttttaaca
    24001 aggaaattga atattctatc tattcatcgg atgctcacgg caaaatgata ttagccgatg
    24061 gactcaaaga atctgtagaa gcaaaatgtg aaattaaact tacaaaagta aaatccattt
    24121 cttcggatga attagcaaaa ttaaaatttc agcatccttt tatcgatcgc atatcgattc
    24181 ctctttttgg gaaccatgtt accttagaag cgggaactgg ttgtgttcac actgctcctg
    24241 gtcacgggat ggacgattat aaagtaggac ttgcggcaga tcttgaagca tttagccctg
    24301 tagatgatcg aggtaaatac actcacgagt ttcctttgat ggaaggagtt caagtctttg
    24361 aagcaaatcc aaagattgta gaattactca aagaaaaaaa tgcgttagtg cattattcca
    24421 cctttgaaca tcaatatcca catagctgga gaagtaaaaa gcctcttatc tttagagcta
    24481 ctccacagtg gttttttaaa atcgatgaaa aagatttaag aaaaaaatct ctagaagcaa
    24541 tcgataaagt aaaatggatt ccagattggg gaatcactcg tattcgctcc atggtagaga
    24601 atcgtccaga ttggtgtcta tcgcgtcaaa gaaattgggg agttccgatt ccagcctttc
    24661 gtcttgcgaa tggagaaact ttcatgactc cagaaacagt tcaatacttt gtagatatcg
    24721 tcaaaaaaga aggtatcgaa gtttggtata cgagagaacc taaagactta ctaccaccta
    24781 acatcaaatt gccgtttgga gacgtaaagg atgttatcaa agaaaatgac attctagatg
    24841 tttggtttga ttcaggtgtt tctaattttg ccgtcttaga atacaaagga gattctcctg
    24901 cggatcttta tttagaagga acagatcagc acagaggttg gtttcaatct agtctctggc
    24961 cttctatggc gttacgcgat aagccccctt ttaaatcagt gcttactcat gggtatgtgt
    25021 tagatgagaa aggtcatgca atgtctaagt cactcggaaa tgtgatcaat cctactacag
    25081 atattatcaa tgtatatgga gccgatattt taagactttg ggtgtcttcc caagacttta
    25141 gagatgatgt aaaaatcgga aaagaaagta ttaagaatgt aagcgagcag tatcgtaaga
    25201 ttcgaaatac ttttcgttat ctacttggaa atttagcagg acatacagca gcggataatc
    25261 tcagttatcc tgagatgcag gaaatcgatc aatactatct tgctcttcta tacaatctat
    25321 ctgttgaatt gaaaaattcc tacgaaacct atcaatttca ccaaatctac caaaaggttt
    25381 taaaattttg tgtaagcgaa ctttcacagg attattttga aatcatccga gatagaatgt
    25441 attgtgatcg aagagattct aaaacaagac gctcttcttg cactgctctt tcaatcattt
    25501 tagagacgtt agtagtatta cttgctcctg ttttaagttt tacagcagaa gaagtttggg
    25561 aaaattacgg gaagaaaact tctgtgtttt tagaaaaatt tccagactta agccaattca
    25621 aaaacgaagt tttaattgct aaattcgaaa aagttttcga aaccaaagaa ctcgttcaaa
    25681 aagcactcga aaacgctcgt caaaatggta agattggtaa atcactcgaa gcaaaactat
    25741 ttatcagcta taaaaatact tctattttag aaaaactatt ttcaagagaa gactttgaat
    25801 tgttctatgt tgtctcccaa gttgaatttg aaccgattgg aaattcagtc accgatgtag
    25861 gggacgcata tatgcctccc ctactgagtt ccgcagagaa tgaagatttc cttgtagaag
    25921 taacgcttcc tacaaatcca gaatgtcctc gctgctggcg gcatacacgc gatatccaca
    25981 aaggtggatt gtgtgagaga tgtaaggcgg cggtttagga ttttaccacc gatgaacacg
    26041 gatgaacacc gatgggattt ttttcttgtc ggtgttgatt gtggtgaatt ttaatatgaa
    26101 ttttttgtaa ctctcgaaaa gtaagaactt attattaatt gtattgagtt atgccgcaat
    26161 atattaaacc taaaaaaaat gaaatctact tagatacatt tcaagaatta tatccaaaat
    26221 ttaacagtga gatcaagaca aaggaattca atttaatttt tgaatattta gttgataata
    26281 tagataaatt agtttgggca attgaggatc ttgactcacc ccctttagag ggagtaggaa
    26341 aagcgttagt ttcgttaaat cctaaaattt ttaacaaccc tgatactcgc agaatgacag
    26401 gtgctctcat tgcagcaatt ttacatcatc atggttacat gcctgcaaca tctggagttc
    26461 gtgtctcttg gaaacctttt attactgcat ctaaatataa aaagaaatag aaaatactta
    26521 ataaatttac caataaggat aatttattag ttgcattcta tatgactatt tgcaatatta
    26581 cttattatat tattgaaaga gatagacatg gaatatttaa ataaatatca attaatgctc
    26641 aaattaaaag actttataga gattcatgaa aaaacaaaga tttccgatct caatgaaggt
    26701 gttttaaaaa tgtatatcct gcattcgaaa aatcaaaata atactcctaa agaaaaatta
    26761 attaattata aattactaag aatagacgaa agacttttga ataatagccc tgaagaaatt
    26821 agcgaacggg attctatcat cgttgaattt ttgattgaag agctaatgaa gtattacgca
    26881 cgaagtaaga agaaataaaa aagaggtaaa ctatgacaga aattctaagt caagaagaaa
    26941 tagatgctct tctagatgcg attggaatgg atcccgaacc tcagatagat acattacaag
    27001 atttatcaaa ctatttaatt caaaaaagaa tagaaaaaaa gaaaaattcg tttgaagaac
    27061 aatatgtaaa tttatttcag ggaccacata ttcaggttac taaaagtctt atcaacaatg
    27121 tttatacaca ggcagctgtt aaagagccaa aagcaattgt taaaattgga aatatagaaa
    27181 ttattccaat caacatttgt cctacgtgta acaactatca ttatgatgaa gaagtccaag
    27241 aaatctattc tgaatcaaag ccggataaat cctttattag taaaaataaa aaattaaaag
    27301 aatatgattt aattaaaaaa tggaaaatgg aaaatagaac aatatgttgt aagaaatgta
    27361 aaacttactt tcagccgacg atagtttata gcaaaaccca taattcaatt ccctatttct
    27421 gtaagctgca aacagttaaa actttagatg actggttttt aacggaaaga aaatcaaaaa
    27481 ttcaattatt ttctttaaag acaaagacta ttaaaataaa taaaaataga acttcttttc
    27541 tttggaatgt attcttaaat aaaaatataa atggaatgga aacttgtccg caagcgttat
    27601 tgactaatat agtaagatat acaccaatag agcatttaga gaaaatgcta agaaatgaaa
    27661 agaatattcc tgttttcaac tcatttgatt ttacacctta ctataaacct actcaaatat
    27721 ttaatgagat ataaaaatga attctattta taattttttc caagatgcaa tttagaaagt
    27781 atagcgttaa atgttatttg aaattaaata tctttcttgt ttcttttgaa acaccattat
    27841 gattacttga tatctagtag tattatactt atgaggaata gaaacatgaa ttttgaatta
    27901 tattatggga aacctggaaa gattgattat gatacaataa tcaatatgtt tccaaagaat
    27961 agtatcaata gtgttcgcac ttcaagccta ccactactgg tatattggaa agatacagat
    28021 aagaaattaa aaaaaatatt gagcttttta aaacttaata gtagcgacaa atactcatta
    28081 tgctttgaat atcctacttc tagcggggcg aaagggaaag cttctatgac agatctaatg
    28141 atactttctg attctaaaaa aattgcgatt gaagctaaat ttacagaagt tacgggaaaa
    28201 tatgaagtaa tagaaaattg gttacagaat ccaaataaag ataacagaaa aaatgttttg
    28261 gagaactgga ttcagatgat tcagccattt tcttcatcgg aattaaaaat tgaaaacata
    28321 aaaacaattc cctaccaatt tttacatcga acagcttcag cttgcgctta ctatgaatct
    28381 aaagaagttt atgttattta tcaaatcttc tatgatggag aaaccaaagc tgaattagaa
    28441 aaatttaaaa aagaaattat taaagctaaa gagattatta atccaaatga aaaattaaaa
    28501 ttttatcttt gggaactaga agtgaagcaa tgtattgaag aggcggacaa agagtatgct
    28561 tttgaacaaa taaaaaccaa aaacatttat gaatttcttc gagatgagat tgttgaaata
    28621 taaagtctac tgctattagc cgtaaaatgt cttgctttaa taattaaaaa taaaagaggt
    28681 ttacaatgac aaaactaact ttaaaaataa tttatctatt cattgcaatt ctacttatct
    28741 catgtggaaa taagatggct ggcaaatgga attgcacagg gggaatggtg agtggattag
    28801 agtttttaag cgacgaccaa gtaatcttgg gaattatggg agcaaaaaca actactacat
    28861 atgtgaaaaa taaagacatg atcaatatca aaactgatta agcggatgtc gtatttaaaa
    28921 tcatcggcga tgaactaaaa ggcgaaacgt atgttggttt tatggaagta ggaaattgta
    28981 aaaagtaata ttcttacaaa tcactacaca tactccatat aaacccaatc tctatcttct
    29041 agtaatttta gaaattcata gaacttttta attctgtatt gcaagtcctc tatacttgta
    29101 tctaaggagc tagctttgag taacccgggt ttagaactag attccaattt taaaactaaa
    29161 tcatagcttt cagagagaat acatttttga aaaaaagaaa tatataaatt tcggttaagc
    29221 tctgtaagtt cgattgcttg aatttcttcc gtttcgtctc cttcaatttc tactttataa
    29281 acattagaag tttgtatttt agaaaattca gaaagtgcta accatttata aaaggaatca
    29341 gatacctcta aaacatattg ttttccattc tttgtataaa aaataatttc cattcttaaa
    29401 tctccatact ctttacattt gcaaattcgc tcgtcggaat attctctgcc caggcaatcc
    29461 cagcttcatc aaatagtttt tttgcgttgg gagaaattgt tccagttgta actacagcgg
    29521 aaatagttcc cttttcaatt aacgttaacg ctaatcctct attgattttt tctctagttt
    29581 taatttctat ataaggcatt attttttatt atactttcta tttaaatcac gaacgatttc
    29641 ctttactttt tctgtatttt tctcattcgg ataataaata tcaccattgg gatcattttt
    29701 ataagttctt tctgattcaa tattttcttt ttgaaaaaga gtaagcatcc aactaatcag
    29761 atttttatga actgttagtt tttgaatcat cttatcttca tctgttggtt tattttgatc
    29821 catcataatc agtgaattga aaaaatattt ctaaagaact agagaactgt aattggtaaa
    29881 gatagtcaaa tattatttaa tttttataaa taaccataca tcaaatttag aatctgcatt
    29941 cgatcccgca ttttaaattg tcggcgatga actaaaagcc caaacgtatg ttggttttat
    30001 ggaagtagga aattgtaaga aagaggcaaa gtgattctag aatttcaccc aatcaatcta
    30061 ctaacttcct ttttcaaatc aggaagatcc tgcaagattg tattataaac aagttcataa
    30121 tcaattccga agtattgatg aattaaaaca ttccgaaagt ctttccagtc ttgccatggg
    30181 aaatttgtat ctgtatttct aatttcatct gataaagcgg cgactgcttc tccgatgatt
    30241 tcaaagcatc taacggtggc attatggatt aaacggtgct ctttaaattg agttaaagaa
    30301 ataccttttg taaaagattc tatatcatct atcgcatcta aaatatgttg caatcttatt
    30361 tcatcacttt cgttcataga tgagttttaa atccttttta atagagggaa atatacgagg
    30421 agatatggaa tccggcgtca aaagatctac cttctttcct aaaagttttt cgagttctct
    30481 tttgtagcga acgaaagtaa gtaagctaat agtaggttca aattctacga gtaaatctaa
    30541 atcgctggat tcttccgcgt ctcctcttgc atacgagcca aacacactcg cttttttcgt
    30601 ttctgttttt gcaagaaagg cacaaacgat ttctattgtt gaatcttcaa ttaacacaaa
    30661 ctaccctact attcgtttga tttctttttt attgactggt tggatgatac catgctctgt
    30721 aacgatagcc gaaatgtatt tggctggcgt tacgtcaaag gcaggattta gagcacgggc
    30781 tttgagtgga gctaaaattc cttttggcaa atacgaattt ccgcgtagat cttttagaaa
    30841 agaattttga gtgagttctt cttcttctct catttcaatt ggaatttcag aaccatcttt
    30901 taaagtaaaa tcaaaactcg tcttggttgc agctacataa aaaggaattt tattttcctt
    30961 tgctacaatg gagagcgcat aggttccgat tttattggct gtatctccgt taggtgcaat
    31021 tcgatccgct cctacaatca ctgcatctat cttcctgttt tgaaaaagcc acgcactcat
    31081 tccttctgct atgatgaagc aattgatctt ttcttcctgc atttcccaag ccgtcagtct
    31141 agatccttgt tgatacggtc ttgtctcatc tgcatagact gtgattttat aaccagcatc
    31201 tctcagagat cgaataatcc cgattgcagt tccatgccct gctgtcgcaa gagctccagt
    31261 attgcaatga gttagaatcg aaatttcttt ttgtcgcttt gagaaaagtt ttaatccatt
    31321 ctttccaatt tctaaattcc aatgcaagtc ttgttctaga agagaaattg cgtatttctc
    31381 agaaagtttt atcaaatctg aaattgaatt ggtgctatat gttttctcgt tgatgagttt
    31441 taaataatcc tctaccgcca aacgaagatt aaccgcagtt ggacgggatt ctaaaagaag
    31501 tctggttctt cgaatgaatt tttcatattc tagtttttca cgagcgtttt ttaattctag
    31561 gactaatccg aatattccgc tcattgcaat ggctggcgct ccccgcacaa ccattgactt
    31621 aatacagaaa ataacgtctt caagactcat acaatcaata tattcctttg cgagcggaat
    31681 ctttctttga tctagaagag agagtctatc tcctttccaa tcaattgatt ttagtatgag
    31741 tctttgcgtt ttcatggcta gaggtagaga cagagatacg aagtatcttc tttgactttg
    31801 agttgaaact tttggtttgc ttcgactgta aacgattggt ttttaccaaa atctttccaa
    31861 tcagaagctc ctgggagttt aacagtgagt tgcccggata taacggtcat aatttctttt
    31921 tgggaagttc caaactcata gtcaccggga gccattacac ctaccgttgc aggagcttca
    31981 ctcgttttaa aagcaatcga ttttactttt ccttcaaaat attcatttac cttaaacata
    32041 atctacctat cttaacgcca gttcgatgta acaaaaaatt taaaagttta ccactgatga
    32101 acactgatgg acacggataa aataataata attaaaacca tccgcgttta tctgtgttca
    32161 tcctgccctt cgctccaagt attcgcgacc aatgccttcg catgtgatta aatattcttg
    32221 tatcgaattc acgttatctt aatttcattg gtttcatttt taaaaatttt tccaatgatt
    32281 gtatatacaa ttcttttctc gaaatgattt tatgacaact aaaataaccg cagggtagtt
    32341 atttctggaa ctcaccgagt tcagtttctc ttttctactt gcaattatca aatgcaatct
    32401 ttctatatat ctaatcatga ataaaaacct cttaaatttt ctactcgtct ttactctcac
    32461 aattccattt ctgcaaaatt gtcacgataa agaaaaagat ttagtaattt tatctggctc
    32521 tgagaatgaa tcgatagaac caattttgaa aaatttcgag tcggaaacgg gcattcgaat
    32581 tgaaatgaaa tataaaggct ctgtagatat tatgatggag cttggtaatg aaacatcaaa
    32641 ctttgatgca atctggcctg ccagcactct ctggattagt ctgggtgata aaagtaggaa
    32701 agttaaacat ataaaatcaa ttatgacgtc ccctgtcgct tttggaattc gaaagagtct
    32761 tgctgaagag ttaggttttg ccaaaagaga cgtcagagtc aaagacatat tagccgctat
    32821 tcgaactaaa aaattaaaat ttatcatgac ctcagcaacc caatccaatt caggaacctc
    32881 agcctattta ggctttttat atgcgttact cggcaatcca gatgttataa cagaagaaga
    32941 cctgtctaaa gaaagtctac gaaaagacat gaaagaactt ttaaatggga ttaatcgatc
    33001 ctctgggagt tcaggttggt taaaagaact tttcctttct ggaaaatatg atgcaatggt
    33061 aaactatgaa tctcttctca ttgaaaccaa tcagacgtta gtatctcaag gaaaagaacc
    33121 actttatatt gtttatccaa aagatggaat tgtaatttct gattccccac tcggttttgt
    33181 gaattcagga aatcccaaaa aagaagagtc ttttaaaaaa ctacaagact acttactttc
    33241 agaaaaaatc caaaaacaaa ttttaaatac aggtagaaga gttggatttg gaggaaccat
    33301 tgaggatgga gacaaatctg tatttaatcc taactgggga attgattcaa aacgaattct
    33361 ctcccctatt cagctcccct ctgcagatat gattcgaaaa gctctctcta tgtatcaaac
    33421 tgaatttaga aagagttctt atacagtttt ttgtttagat ttctccgata gtatgctagg
    33481 aacaggagaa agacaattaa aagaagcaat ggatttgctt ttagataaag aaaaagctac
    33541 ccgttatttc ctagactttt ctaaagacga caagattgta gtaattattt ttagtcatat
    33601 cacacttgct atgtggacgg ctaacggcaa caatccagat gaaataaaag aactgaatgc
    33661 aaagattcaa ggcacactac ccaatggagc tactgatatc tactcaccta ttatcgctgc
    33721 tttaaaagaa ttaaaagatg tggataatga tgagtattct cccgctgtaa ttgtgatgac
    33781 agatggaatt tctaatagcg gtaaaaattt taaagacgta agccgagctt ttaagaaaat
    33841 agacaaagat attcctgttt tcccaataat gtttggagat gcttccgaag accaattaaa
    33901 agaaattgcc gaattaacaa gagcaaaagt tttcgatgga agatccaatt taattcaagc
    33961 atttaaaaca gcaaaaggtt acaattaaag aagagatttt aaataaaaat ttgatctaag
    34021 gacaatagaa tgattttaag attattgcag aactcaaatg agataagaaa aacaagagcc
    34081 tgcggcagca agccctccgc taggagcaat catcatgtaa ccgaatggtt cacctttggc
    34141 aaggtaaatt gatgaaggac ttcatcaagg acaaaatcac tgtaaaagga aatcttttcg
    34201 ctgggtttac tggtggggga gtattctctc ttttctttct atttttaaat ataccgttag
    34261 gcgcttccat tacagcatcg attgtatctt atttagctgg actcttgata ttcgcaaagg
    34321 caaaccaaca atttgatata aagctaagcg acgtaaaaat agacgataag cgctctggag
    34381 agattttaca ggaatttaga agaaaaacga atcaactcag aattcattcg aattcaattc
    34441 gaaactctaa aatcaaagca aaggtcgaag acattttatc tgttcttcaa aaactagatg
    34501 ataactttat caaagatcct tctgatattg ctcgcgcgag acaatttctc gtatattata
    34561 tggactcaac agttaaaatc gttcagtttt acattgactt gagtgcacaa aattcgaatt
    34621 caaaagaagt aaaggatgct cttacaaaag gagaatccat tctggattta attttacatt
    34681 cctttgaagt gcaacatgcg aagcttctca gcaatgatgt aatggatttt aatgcagaaa
    34741 tagaagtatt ggaaaaaact ttaaaaatgg agaatatgga ataatggatg atttaaataa
    34801 aattataggc ataatagtgt tagccgttat gatcggtggt ggagttgcat ataaaatgta
    34861 tgactccggt tccgatgcag tggttattcg gggatttatt ggaggagaaa aagcaaactt
    34921 tttagaaaat gaaaaagtaa aaaaaattct taagaataag tataaaatca ttttagaata
    34981 ttcaaaatct ggctccattg aaatggttca aaatactcct tctgacaata cagactttct
    35041 ctggccttct agccaggttg ctcttgaact attcaaaatc aatcatccac aaaagattgt
    35101 aaaaagcgaa atcattctaa attcgcctat cgtattttat agctgggatc tggtgacaga
    35161 cgctctcata aaagacgaaa ttgtttctaa aaaaggaaac tcctactaca tcactgattt
    35221 caaaaaacta attcaaaata tttccaatgg gaagaaatgg tctgaaattg gattaaaaga
    35281 actttatggg aaaatattaa tattatctac tgacccaacc aaatccaatt caggaactat
    35341 gtttgcaggt cttctagcaa acctaatcaa tggggatacg ttagatgaaa cttcttttgc
    35401 agaagtaagt agctctgtca aagattactt tgctcgtctt ggttatatgg aaacttcttc
    35461 ttccgattta tttgagcaat atctaaaaac tggaatggga gcaaagccaa tcattgcagg
    35521 atatgaaaat caaattgtag aattttccat tgagcactcc gacttctggc ccaaagtaaa
    35581 agaaaaaatt agaattttat atcctgttcc aacagtttgg agttcacagc ctctaatcat
    35641 cttaaatcca aaagcggtga atctaatcca agcgctgcag gacaaagaga ttcaacaaat
    35701 tgcttgggaa aaacatggat ttagaactgg actcattgga caacaaaacg atcctaaaat
    35761 tctagaagtg gtaggaattc cagaaagtat cacgcaagta attccaatgc caagtccgac
    35821 tattatggag aaactaatca aagtaattca atcaaattaa tggtaactac tctgcctatc
    35881 ggtttcgtaa ttactttaag attgacagat gcctagtgcg acttccttca atacaggtgt
    35941 cggcaacaga atgaatagtt aaaataaatg aatcttccta tttttgaagt attagacgag
    36001 ctaaaagcaa atctagccaa aactaatctt gtaattttac aagcgccacc tggtgcgggg
    36061 aaaacaactg ttatcccctt agctctttta gaagaatcgt ggctcaaaga caaatcaatt
    36121 ctagttcttg aaccaagaag actcgcagct ataaatgctg ctaatagaat gtcaaagtct
    36181 atcggagaag tagctggagg ggtagtcggc tatcgcattc gttttgattc aaaggtaagt
    36241 aataagacaa aaattgaagt tgttacagag ggaattttta caagaaggat tcagtctgat
    36301 ccagaactta agaacattgg ccttgttatc tttgatgagt ttcacgaaag aaatttacaa
    36361 acagatttag gtcttgcctt tgctttagaa gtgcaagggg cttatagaga agatttaaaa
    36421 attttaatca tgtctgctac tttagatgga aatcaccttt ctaatttttt ttcagcacca
    36481 ctgatagaga gccaaggtaa agcctttccg gttgaggtaa aacatcttgg actttcaaaa
    36541 gaaaaattaa attcatcaat tcttacagca attcaaaaag ctttaaaaga aaaaggagat
    36601 attctggtct ttctcccggg atcaggcgaa attaaaaaaa ttcaaagaga aatagaagaa
    36661 aatttgaatc ttactgatac tcttatttat ccgctgtatg gagatttatc acagaaagag
    36721 caagaacttg caatcttccc agacaaatca ggaaaaagaa aaatcatact ctctacaaac
    36781 atcgcagaat ccagtcttac cattgaagga gtaagcatag tcattgactc aggtctcgca
    36841 agagttcctc gcttcgatcc ttctactgga atgacagaac ttgtaaccat tcaaattgca
    36901 aaagattcaa tggaacaaag aacaggaaga gccggaagac ttggacccgg aatctcctat
    36961 cgcatttggt caaaagagga agaaagaaat ttctctgaaa aaacaaagcc agaaatttta
    37021 gaaacagaac tttcctcttt tctattagaa atactcaatt ggggatcaag actaaaagac
    37081 ttaaaatttt tagacatgcc atcggaaagt tccgttagtc gctctaaatc cctactccta
    37141 agtcttaatc taatagataa acaagaaaag ataactgatc tgggcaagga aatcaatcga
    37201 attccacttc atccccgtct tggctatatg ctctatcaaa caaaagatac caaagaattt
    37261 tatttggctt gtcttattgc ttcaatttta acagagaaga gtttatttac agcaaatcat
    37321 ttttcattta aacaatcaga tatagctctt gaggtagaag aatttctaaa aaatcagaat
    37381 agctatttaa ctcaaaaaat taaactctcc actgaagaac taatgcgaac gctccagaca
    37441 gaatctaaat ctaagactga aaacatagat atctctcaaa ttggaaagat ccttagcttt
    37501 gcgtatatcg atcgaattgc aaaacagcga gaaaaaaata gcaatcgctt taaactctca
    37561 tcagggaaag gtgccttttt aagagaaaat gattcactca ttggaaatga atttttggtt
    37621 gtcgcatctc tcgatggaga taaaaaagaa gcagctattt ttaaagcagc aaagatttct
    37681 ctagaagaaa ttctttctac tcactctaat aaaatcttaa aacaagaaag ggctttctta
    37741 gaggaaaagt caggaaagat cttagtgcaa gaagaagttg ttctaggaga acttgtatta
    37801 tcagcaaaag aaaaggattc taaaatcaca gaagaattgc tttcaaatct ttggattgat
    37861 tacataaaag agagaggttt atcggtatta gcctacacag aagaatctaa aaatttcaaa
    37921 gaaagatctg aatttttgcg atctaacgga gaagatttac ctgatttatc ggatgaatat
    37981 ttacttttac atttagaaga ttggttaagt ccttatctaa tttctattcg taaactctcc
    38041 gaactaagaa atttggactt actttccatt tttaaatctt tgttttccta cgaccagata
    38101 caattaatcg aaaaagaggc accagaaaga tggactgttc caagtggatc aaaaattaaa
    38161 cttgaatatc gaaaagagga agtaatttta tctgtaaaat tgcaagagct atttggatta
    38221 aaagaaactc ccactatagc aaagggtaag gttaaattga ctctcgaatt actttccccc
    38281 tcacagcgtc ctattcaaat tacaaaagac ttagcaggat tttgggaaag gacttattta
    38341 gaagtaaaaa aggaattagc gggacgatat ccgaaacatc catggccgga caatccgctc
    38401 gaagcccaag caactcgttt tactaagaat agaattaatc agacttagac tttaatttat
    38461 ttaaggcaaa tcgtcctcta aattcacgca cacgtaaatg aatgagttcg cttagggtat
    38521 ctaccatttt ctttttgttc atatcttcga tacgagcttt tattttttct tttactgtgt
    38581 agaaagtgtt tgctttgatt tcttttggta atgtcccaaa aagagatttt aaaattttaa
    38641 tatgagtttc agttaaaatg cctttctcat cttctgcaat acatttatct acaaactcag
    38701 acaaaggcat ttcttgtaat gtattatcat gctcagataa aatcagatag tagatagtat
    38761 gaatttcacg aagaggaatt ctgagtttct cttccagatt ttgaaagaag aatgctaaat
    38821 cttctaatgt ttttttagga tcctttgcta cttctttcat gataaggtaa atgagcgtta
    38881 tgtgcttttc atctacatca ggaatatctt tctttagtct cagtgtaata ctttttatgg
    38941 cttcctgtat atttacatta gaagttgggt tcttaaataa agatctaatt tcaaataata
    39001 aaatatcaac gatataagga taatccagtt tcgtatttaa taattccgct ccaggtcctt
    39061 gtgattctgt aagagcaact aattttgcat cggaatcact catgtccgaa ttttcttctg
    39121 cgactacact ttctgttgat tcggaactac cgttagccgc atactctgcc gcaaccgtta
    39181 atagattttg tgccgcaaaa aaaacagttt gcacataccc accaactaag cgaatgcgat
    39241 ctaagaattt aggcatatta tttccattct taatccaagt taaacattta cgaacttgat
    39301 caggtcctga cttggaaaag aaactaatat cacctgaaga aataaacgga aatatatttt
    39361 gatttgctat aaatttttga acatcagtta ttttgatatt tgctgtgtgt atgtctgccg
    39421 tgtccgtgct aataaaggag atttgggaag gattgcgagt aattttaaaa aaatcctctt
    39481 catcccagag ccgtttgaca aggatatgaa taatttgatt ataaccaatg gcttttaatt
    39541 tattttgttt tggtggcgca aaaaaataag atttgttaga tagcttgtcg agaaaaacct
    39601 ttctagtttc ttctaagatt cttttatcat aacgggaacc tctatcaaaa tttaaataca
    39661 ttttgagtgc atttgcaaca gtattctcat cactttgatt gagtataatt tgcccttcta
    39721 aatcatctaa caattttgcc atataaaccg tctaattcct ttctaacaat cctacttaaa
    39781 ttcttcaaga aattatttca ttactccatg taagaatcaa aacagaaact aaatatgaaa
    39841 aagattacaa aggagagcat cgttaagaga acgattttaa acaaagattt tgaaagaaat
    39901 atcgtttttt ttatcgatgt aagtggtgat tgcttgtaat ctttgcaagt ccctctattt
    39961 tacagcattg aataggaaga ttcgtctaca ctcatagtat ttcttagtta aaaatggttc
    40021 taaacctaaa aagtaattgc tatccatatc agaatattta taaaataccg gaactttatt
    40081 gaaggaaaaa gcgaatggaa aacatacctc aggaaatcat tgatagaatc aacactctca
    40141 aacaagatca aaaggaaaaa gttatcaaat atatcaaaag ggaaattgaa gtgggtcgaa
    40201 tcccctctgt agatgaactt tctagtatca ttcgctattt aaatgaatcc gaaaaaaaag
    40261 gaacacttta taagatcgaa ggaattgcaa tcgagccaga taaacctaag aaaactctgc
    40321 aagttactga aaaatcgaat tgccttgcct gtcgattcca agaatctgga atctgtaaaa
    40381 ataaaaaatc ggagttattt gaatccaaag tgtttgcttc cataatttgc gttcactttg
    40441 ctactaaaac ctagcatgag cttaaaaacc tataaagatg gaaataagca aaaccctact
    40501 ataatttttt tacatggctt tcctctcgat catagaatgt ggaaatccca aatcgaattt
    40561 ttaaaagata aatattactg tattagctac gatattcttc aaaaattaga aaaatctaaa
    40621 aatccagagc caattccctt tgaattcctt gtagatgatt ttctcaattt attagagaag
    40681 aaaaagattc aaaagcctat cgtatgtggc ttatcgatgg gaggctatat tctattgcgc
    40741 gcaattgaaa aaaagccaga tgcattttcc aaactaattc tttgtaatac aaagacggaa
    40801 gcggatacga atgaaggtaa attaaaaaga gtcgatggta ttcgaaaaat caattcagaa
    40861 ggattaaagg attttataaa agaatttacc acaaacgcac tttgtgaatt tacctgtaag
    40921 gcaaaccctg agatttacaa gtcagcatta aaaattgcga aagatagaac tcctaacgca
    40981 acaaaagcaa tcctactcgc tatgcaaggt agaaccgata ccactcatgt tctaaaaaca
    41041 atttcgattc caactttagt aattggggga gaatacgatt cacttactcc acctgaatct
    41101 atggaaaaat tagcaaacca aattccaaat tctagttttg taaaaattcc tgatgcagga
    41161 cactttgctc catttgaaaa tcctaaattt acgaatcaaa aaattttgga atttctggaa
    41221 aaatgaaatt tatcttattt ttatttttta tcatacttac tccgattttc tccgaagaca
    41281 atctaggttt taaaaagaaa caaaacattg atactaatga attggaagtt ttctctgaaa
    41341 aattttttag agaaaagaaa ttagaaggca aacatgtatt agtcgcaatc gtaaaagata
    41401 ataagtcatt ctttaaggta tatcccgaac attcaaaagc ttttacagaa ttaacacaat
    41461 ttaactctgt tgtatttgat gagatttttt taacattgca tactttacat ttagaggaaa
    41521 ttggaaaatt aaatctccga tcagatatat ataattataa atggaaatac aatcgaaact
    41581 ttaagcaaag cattctgatt gaaaatcttt taactcacac aactggaatt caagaaaatt
    41641 taatctattc caatttggaa aaagaaagac caaaagattt ttggaagaat cagttacctg
    41701 caagatttgc tgaaccaaat gagttaatca gttacaatcc agatcattca gtaattttaa
    41761 aatcaatttt agaagaggta aattctaaaa gtttagatga agttatgaaa gaaaccattc
    41821 tctctaagct agaattagaa aaaacaaatt atgaaaacca aaagctcaat ataaatgcaa
    41881 aggacattga gaaactttta tttctttttc taaatccaga ccttcttgct caaaatgata
    41941 ttttaaaaaa agaaacatgg aattcgatgt ttcaagagaa aattcgttta gaagataaat
    42001 taccaggttc cgtgatagga ttttttgaac attatgaaaa taaaatttgg ggctattcta
    42061 gaatagcggc ttccaattta tcaacagaag aatttatttt tttcccagat aagaatacta
    42121 gcttttatat atatacagac tcgtttgatc cagatttacg aatagaatgg aaaagtgaat
    42181 ttttagacca tttctttcag aataaaaaag taaaagcaaa gaaagataag aatccaggat
    42241 atgaagaata ccttaagaat tttgaaggtg aatatgcgtc agtttccatt tcttctcaaa
    42301 atatttccaa attcagaaac tactcttccg caatacaaat caaacaagaa aatagacttc
    42361 ttgttctaga atcaggtgaa attgaaccct atggagattt acacggtagg cttgaattta
    42421 tagagattga tcctttttta tttagatgtt tagatagaga aagctatatt tctttcaaaa
    42481 tgaatgaagc aggagatgta gaatatctac tttcaggatc agggcaacat ggggcttacc
    42541 gcaaattacc tttccatgaa aaaaaatcat ttcaagaatc tattctctat tttttcattg
    42601 ggttttattc catctactta attcttctta taatagaagt tccttattct tttgctaaaa
    42661 agatcgcatt tgttcagaat agaatgagaa gaaatacaat gattttactt tggatcgaaa
    42721 cgttacttgc aatctctact tttgttggat tttattactt tattcaagaa aacaaattat
    42781 ctcaagttag tgagtatatt ttaactggta atagttttga ttatgccctc tttactctac
    42841 ctcttttgtt tatatttgta ttgagtttct tggcatacta ttccttaaag tcttttctag
    42901 aaaaaggtct tcatctctac aggcaaattc aaaccatttt gttcttagtt gtttcccttg
    42961 gatttatcta ctggatgagc tactggaacc tacttgtttt tttatttaac tgatcagcgg
    43021 ataaatagta tttacctcag taatttattt ttccgacata ggaacctgaa tctgaaagat
    43081 tgatcgattg aaagatttgg ttagaactaa tctattcaga aatcagtgtt ttagaattta
    43141 aaaagggaag gattaaagag attgagaagt agaaatcggg gacatatgtc cccgatatta
    43201 aaattagttt tattttccag aagcagcttc tttagtaacg taagcagcaa tttcattctt
    43261 ttggatttga gttgttccct caaagataca aagaatttta gcatcgcgca ttaatttttc
    43321 tactggatat tcttttgtat aaccatatcc accatagatt tgaactgcgt ctaacgcaca
    43381 tttcatagca ccttctgaag tgaatgcttt tgcgatagca gagtattttg ctaggttagg
    43441 atcacccgca tcagacatac gagctgcacg ataagtaatc tcacgaccaa cttctacagt
    43501 aatagacatg tctgctaaca tatgttgaac agcttggaaa gttccaattt tttgaccaaa
    43561 ttgttctctt tctctagcat atttagaagc ataatcaaga gcagcttgcc ccactccaac
    43621 tcccattgca gcaacgaatg gacgagatgc gtttaaggta tgtaatgcat acacaaatcc
    43681 catattttcg cgaccaatta aattttcagc aggaactgca caatcttcga aaatgatttg
    43741 tcttgtatca gaagcacgaa ttcctaattt gtcttctttt tttccaacga taagtcctgg
    43801 agtatttctt ggaacataaa agcaactaac cccacgagtt ccacgatttc tatcagtgta
    43861 ggcaaacaca gtgtatgcgt cagcgcttcc accaccagtg atccattgtt ttgctccatt
    43921 gatcacatac ttatcaccct tcttttctgc tttggtcatc atcccaggaa cgtcagaacc
    43981 agctcctggc tcagagagac aaaaagaaat tccatgttct ccagcaatca caggctcgag
    44041 ccattttttc ttttgtgcat cggttccacc ttttaaaatt ggaagttgtc cgagaccagt
    44101 gtatccaaaa cataatgcga tacctagaca accacggctc atctcttctg tgatcagaca
    44161 ttgttctacg gaaccatatc ccataccacc atactcaacg ggaatggtca aaccattgat
    44221 acctaactct cttctcattc tatcaatgag ttctttagga tgttcattct tctcgtcgta
    44281 gatgtgagca actggcgtaa tttcttttct tgtaaaatct cttactaagt ctctgatttg
    44341 aatttgatct tctgtaaagg cttgatacat aattttattc ccttgttatt ggtagtttta
    44401 ttatagtttt tttcttctga cattggtttg tgtcagttaa caaatcctat tattttgtat
    44461 tatgatttta aatcaaactt aatatttgta gaattagacc gattggttca aaaatgaact
    44521 gattaatgtc aatgtttaca ctgctaactt ttatgttgaa ttctgcaatg tagctgattt
    44581 ttgaatctat aatcttgaat ttacattttt cttaggaatg aaaaccattt cagaaagata
    44641 aatatagcct ctcaatacag aatttacaat gcattcaaat cattatttat gttgcttttc
    44701 atctgtaatt aaaaaggatt taaacctatg attcgaattt tgatttttct ttttttaatt
    44761 ggtttatttt tctgctgtct acaaaattca aaacatttga atccacctac aaaaacaata
    44821 caaacctatt ttataaactc tgattcaggt ttaaaaatgc gaatagagcc agatcaaaaa
    44881 tccaagacaa tccatactat tttcaatgga gaagaaatta taatactcga agaaaaaaca
    44941 gaaaaagtta ttattgataa tatagaaaac agctgggtca aaattcaaaa caaagaaaag
    45001 ataggctggg tttttaaagg atacctctcg gaaaaaaatc cgtatattgg taaatataaa
    45061 atgaaaattg tgaattatgc tcaatgcaaa tatccagaat ttttcttgat tgaggaaaat
    45121 aatttatggg agggaacttt ttttatcggt ggagatgaag gagattgtgg ttatacagat
    45181 atcaatggga cttggtatct aaaacaaaac caaatttgtt ttaaaattaa agaagccagt
    45241 tcctctcatg atcattttta taaaggtatt gatccctgtt attacttaca aaaaaatact
    45301 ttgtatgcgg ttaatgatga ttatgcgttt aaggaaaatt ttggcagcac aatagtatcc
    45361 ggtattaaga aaactgaata ataaaattcc tatgattaca atacgacctg caaataaaaa
    45421 tgatctttcc cttttacaaa aatgggatga agaaccccat gtgattgaat ctgatccaaa
    45481 cgatgactgg gaatgggaaa aagaattaga tcgcaatccc gaatggaggg aacaactcat
    45541 tgcggaacta gatggcagac ctattggatt tatacaaatc attgatcccg cactcgaaga
    45601 cagtcattac tggggagatg tttccagcca cttgagagcc attgacattt ggattgggga
    45661 caaaaacgac cttggaaaag gttatggatc agtcatgatg gaattagcaa tagagaaatg
    45721 ttttaatgac aaagaagtga aatcaatttt aattgaccct ttagaatcta acgttctcgc
    45781 ccataaattc tatgaaaaaa taggattcaa atttatccga aaacaaaaat tcggagaaga
    45841 caattgtttt gtatatagcc tagatagatt cgattgggaa aataaaaaga aataaaatca
    45901 tttttccctt ttccaaattt tccctgtttt cttttctctg caaaaggata aggaagtaaa
    45961 gattaagaat agtaaatata aacgtaaaac aagtttcgga taatgcataa ttgcctctag
    46021 caatagaatg agatttgcaa ttagaaatta aattgtcact ggatttttat attactgaaa
    46081 ctgtcatttc gaacatcgct aggcaactag atttgtggct aagtaaatga gtggatgatg
    46141 aagagggtca aggtgacttt gtaactcgca tccctttatc ggtattattc tttataaatt
    46201 cattatgtat tttaattaac attttcatca attgaaatac ataatggtag tatgtatacc
    46261 gatatgcaaa gacgagagtt ttttcacttc gctttcctag tgcgattttt gaaattgaca
    46321 gacccaaatc tttatctgga aaaaaaaatt gggacggttt acgaagtcga gtattagtaa
    46381 aactttatga acttacataa gtattccatt ttacaatcct cacccatttt tacgacgcat
    46441 gaatttgcct tacaaacaaa tacttcaatg agttcagcag cgcgacttct aacacgaata
    46501 gaagaaagaa aagaaatcaa tcgagtcact cgtggtattt ggacttatac caaagaacaa
    46561 aaagttcatc cattttcagt cacgccttat cttttgggaa aagaaattgg gtatatatca
    46621 ctattatccg ctctgcacat tcatggaatt ctttcacaaa ttccaacccg catccaagtt
    46681 gtaacgatag cacattctag acttgtaaaa acaaaattag gagtatttga attttttcag
    46741 atgaaatcaa gtatgatgac tgaaggagtt acttggtctg atacaaaact tccctttcga
    46801 atagcgacac ccgagaaagc tcttttagat gttttctatt tgagcactcg aaaaggaaaa
    46861 cgcttttact ctctacctga attagaatta aaggaatcag gatttaagaa aaaagtattt
    46921 ccaaatcttt taaccaatca gaatttttcc aaaccaattc acaatgcaat tttacagaga
    46981 tttaaaaact tacagaatca ttaaaacaat atgagttcga tttaaatatc ttactcaggg
    47041 tgtttttaca aaaaaagtga ttagacaaaa gaattttctc actatcgaca gttttaagat
    47101 tctttattta tcggtgtcct cgtttcgtat cggtgcaatc tttgcaaaat caaactaaag
    47161 ttaaaattat ttctgcataa gctcatttaa ttgtgataca ggaagaactt ctattttttt
    47221 atgtatcata taacgatctt ctccaggata tacaacccag atcttttcta attttaaatc
    47281 attcatagca atctgcatgg atttagtcag agttggagcg tcagaatatt ttacttcgac
    47341 tccccacctc ttccctccct tcatccagaa taagtccaat tcagttcctt gatgagttct
    47401 atagaaaaaa taatttacta actcttttct ctgcatcgaa ataatttgat taattacaaa
    47461 cccctcaaag gaagcgccta tctttggatg agcttgaagt tgggaaaatt tttctataaa
    47521 ttgcaaagta tgaaaaattc cagtatcact aagataaatt ttgggctgct ttgtaagtct
    47581 tttacctgta ttttcatgcc aaggttgcaa aacgtttaac ataaatgtat tttttaaaat
    47641 atcaatgtat tttcgaacag tcatatcaga gattccaaaa gaatgggcta actctgcata
    47701 atttaataag cctccatgat aatgactaat cataatccaa aatcttctaa gtgtataagc
    47761 tggtatcgta attcccaatt gaggaatatc tttctctagg aatgttgtta tgtattgctc
    47821 tcgccaagca taactcttat tagccgcttt tgccagaaat gacattggaa aagaacctct
    47881 ttcccacaat cgtttccaat ccgttttttt aatttcactt aaatcaaaac catatacagg
    47941 agtataatag attcttccag caagggtctc agacgcctgt cgtatcaatt ctcgagaagc
    48001 gcttcccgaa acaagaaacc tagttttatt ttttggtcta tcaacgagaa atcttaataa
    48061 tgtaaatagt tcaggctttc tctgaatttc atcgatcaca acaacaccag ataaattttc
    48121 taatagtagc tgaggatttt caaatttagc taaatctctc ggattttcca aatcaaaatg
    48181 atatttactt ttaacaagtc gagcaattgt agtttttcct acttgcctag ctcctatgag
    48241 agctactact ggaaagtcac ggagggcttt caagatgtcc tttgcgtaaa tatttctatc
    48301 catgaaatat catatatcat atatgatatt tcatgtaaac aaataatagc tattttctta
    48361 ttttttgaat gattcgatac agaatcatag atagtattcc accaatgaaa aaggatatag
    48421 gtgcaaaaat aaatagaaca aatccaaaaa ttattccggt gctgagcttc cgtatacaga
    48481 ttcagtcaaa tctagcaaaa tataaaaaca ggaagcgatt gttgatagaa tccctccaat
    48541 aaaaaaaaca aaatgtgatt taggttcacc acttacacta attttctaaa acgtatataa
    48601 aagtccgaat aaccacgatt agaccaaaag cgaactgcat ctctattttc tgtgagagct
    48661 cttagctcaa tgactttaat cttttttttc tttgcccagc tttctgtgaa atcaagaagg
    48721 gaatgcatat atcctttttt catttgtcct cgcttcgtta cggcaagatc tatatataaa
    48781 attttatctt cctccaaatg aggtctttct tctattcttg caatcaaaag tgagacaagt
    48841 tcatcgtcta gaactgctcc aataaaaaca attttacctg tcccttgtaa ttttaagtaa
    48901 atatccacca ttttagatgc ggctttcgat tttaccttaa acactccatc taatttcatt
    48961 gcattgattt gtttaaagaa ttgattcact aactcgattg cctgtgttct aaatttagaa
    49021 tcaatgttga ttattttaaa ttctgtttgc ataatattcc atttatttgt ctaatcatta
    49081 aaaagaaaag gaatcctaat tgaaagtaaa ccaattttta tcaaccctca ccaaccttga
    49141 aaaaactcgc aatttcaatg tattcaaaga atactcactt gaagaattta aatctgttat
    49201 gaatcatttt caattacttg aatcaagtag aaataaactt aggatttcta ttgttggaac
    49261 aaatggaaag ggatctactg cttttttact aagtcaagtt ttttcaagat tggagggcaa
    49321 ttcgcctgtc ggtttataca cttctcctca tttaatcgtt caaaatgaga gaatccaagt
    49381 tgatggaatt atgatatcgg atgaatggat tgaaaatttt ctaaattctt tccatgcaga
    49441 tgatttagca aaacttagaa atctttccta ttttgaattc tttactcttt tatctgtatt
    49501 ctatttcaaa tcaaaagaat gtaaaacaga aatctatgaa gcagggttag gcggtaggct
    49561 tgatgcaact aaattaatta atccagaaat tgtagttctt acaaaaattg gtcttgatca
    49621 cacagaaata cttggaagca cagaggaaaa gattctccat gaaaaattat tgatttcagg
    49681 aacaaacact cgtttcctat ttacattcaa acaatccaat aaactcatgg aaattataaa
    49741 agacttttgt aatgagaaaa atattggttt atacgtatac gataaaacga tccaaacaga
    49801 ttaccttaca tttaatcgtg acttcgcact ttttatagca aatgaaattc taaaaacaaa
    49861 aaataaacca ccactcggag atactttttt taattcaatg catgacgttc caggaagaat
    49921 gcaagtctta cgaaagaatc caccacttat ttttgatgta gggcataatc cttctgcgat
    49981 tccttatgtt ctgcaatctc tacaatttct ctatccaaat gaaaaaaact ggtctgtttg
    50041 ttttggagct ttgaaagata aagatgttat aaaaatacta gaaatactta cagcaagtcc
    50101 tatcatcaaa aaagtttttc aaattgtggg acagagctgg aatataacaa ccattagcca
    50161 caaaaaaatt aaattgattc aagaagagga ttttttagat tcgattccga gtgaggctgg
    50221 attaatacta ggcagttttc ggctttatcc tccagtcaaa caaaatgaat tttagtcttt
    50281 ctttattttt cattcattta gcaagttggt tttttcttta tatatttcca cttcttcttg
    50341 tatcgattgt catgattaca atgggattgt tactcagaca tttaattttc actgcaaaag
    50401 gaaatcgaat tcgtcataga aaggttctaa cctatgaaga aaaacgcgca gaaattgagg
    50461 attttagtat aaaaattact tttatattcc gagcattttt tataggatta acactggctg
    50521 caatcattct ttctgtttat ttgtggcttt atgaaatcta attggattta ttctaatact
    50581 cagggcaata aagcaagatt tctcttggga gagaaaggaa ataaaacgtt agtatgcatt
    50641 ggaataaatc ctagcattgc tgagccagag aaattagata atactttaaa agttgtaaag
    50701 aggttttcta aggatttagg atacgattct tggttgatgt tgaatgttta tccgcagcgt
    50761 gcaacaaatc caaatgaact tgatttcgaa attaatatgg attatcatcg ggaaaattta
    50821 tttcaaataa ataaaatttt aaagcatggc aactatgata tctgggctgc atggggaact
    50881 ataatcaaaa aaagaaaata tcttgtttct tgtttaaagg atatttatat gataacaaga
    50941 aaatattctt tgaaatggaa taagattgga aggttaagca aagaaggaca tccacatcat
    51001 cctttatatt tgaatcgaaa tttaaagctt gaacaatttg atattgataa ctatttaaat
    51061 agattagtgt aatagtaaaa tgtatcgact aaggtatgta gttgatagtc aatttaattt
    51121 ttaaagtaat atatgcgaag tatatttaat acacaggaag acatatttat tgaattggaa
    51181 tattggagaa attgatttat gaattttaaa aaaacaataa gaatattctt actcgatgga
    51241 gaaattaatg gaagattaac ttgtgaactt tccaattgga cgggaaaggc atataaaatt
    51301 ccgagaaaca aaataattgc atctagtgat cgatccgaat tattaaaagc aggagtatac
    51361 atgcttttcg gaaaatctga ggacgatagt tacgaagggg aagcgtatat tggagaatca
    51421 gaaaatattt ttgagagatt aaaacaacat ttaaaagaaa aagaattttg gtatgattca
    51481 atggcaatca taagtaaaga tgaaaattta aataaagctc acattaaata cttagaaaat
    51541 aaaatattct ctattgcaga aaatacaaaa agatttaaaa ttcataatga aaatattcca
    51601 gctaagccat ctatatcaga atcagaaagc gcagagatgg acgaattttt atctaatatc
    51661 aaaattttag taaatacact tggctataaa atatttgatg agactgcttc tgtaagtaaa
    51721 gaaaagaaaa aatcagaaat attttatata tcagcaacga gaggagcaaa ggcaagtgga
    51781 aaatctgttt ccgatggatt tctagtattt aagaattctg aaattgcttt ttcattaaca
    51841 aattcctatc ctgaatcaat gaaaagatt
//
LOCUS       DAOQOS010000025        50734 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_259_length_50734_cov_5.836836, whole genome shotgun sequence.
ACCESSION   DAOQOS010000025 DAOQOS010000000
VERSION     DAOQOS010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 50734)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 50734)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50734
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_259_length_50734_cov_5.836836"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(137..385)
                     /locus_tag="PLF66_07505"
     CDS             complement(137..385)
                     /locus_tag="PLF66_07505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63922.1"
                     /translation="MSGNLIINVILLLFLIDSCDNSEKSICESKSRINKQFFCKDNFI
                     LSADVFDKLKDLILLGCLAAIEEAKKCKNKSPIKPDAL"
     gene            complement(1332..2336)
                     /locus_tag="PLF66_07510"
     CDS             complement(1332..2336)
                     /locus_tag="PLF66_07510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63923.1"
                     /translation="MASLLKNFILLLLLGFTVFLRIENYIITSAKPRLNLYETQKPNP
                     IQSSSKVGSFLSADNVSFRFKENIILLLKNLQAEIIPVEPLKVVNLDDVNAFKIQVVK
                     ADVTIDSSTLKVIMRDNIFNYTDSPLKLKELEFPEKNVDKIKLTGTLHFFIWLDFELN
                     GKISLDEKKEKIVITAEEIHALGNSYAKSLLGAVGLNLEKLLPIPEGRGLVVKGNQIF
                     VEPFAIFPPPKLSGTISNLQILDHKLFLSLDNGKSLLVPQMPNPNSKNFLYFFTGDLK
                     FAKLFMVESSLQIYDKDESDPFDFFMERYLTVLAKAGIVTVGQDRSLKVVMPDYNDAF
                     "
     gene            complement(2339..4552)
                     /locus_tag="PLF66_07515"
     CDS             complement(2339..4552)
                     /locus_tag="PLF66_07515"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HMZ63924.1"
                     /translation="MKKYEPIAYILMGVVFALVMTLVFIKGDRYKSHKEITDPKKLEQ
                     MKKNDAETAASNDSILESFHKEIKKNLKDPLSVVLIQVVVVLLFARFFSMIFKKLGQP
                     SVIGEIFAGIALGPSFIGFLFPEFSQILFPKESFGYLKILSQIGLLIFMFIIGMELDV
                     SVLKKKASSAIFISHTSIIFPYTLGVLLSLYLFDEFAPQGVSFLSFGLFMGIAMSITA
                     FPVLARIIQERGLTKSPLGIMAITCAAVDDISAWCILAIIIAIVNAGTLYTAIITLVI
                     SFFFIAVMIFLIQPLLNRMSKVYITKEIIGKGVLTALLLIVIASAIFAEGIGIHALFG
                     AFLAGVVMPANTKLRNVLTEKFEDFSTIFLLPLFFAFTGIRTKIGLLNDLHLWAVCLL
                     IIIVAIIGKLVGSMFASRFTGMSWRDSFAIGVLMNTRGLMELIVLNIGYDLGIISPTI
                     FVMMVLMALVTTFITGPLLKLSVKTEESEKKEVIPTGSSSPILITFGPTSAGISLLKL
                     ASGISGKNGTVAALHLSPVPENISITSETSIDANFFAPLESIAKEKKLKLKPIFKVSD
                     EIPKEISATVRKEQSTLVLMGAAKRVFGDNVLGGKVESVLSELDCTVGVFLDRNINDI
                     KSVAVFYSDPIEVNSLLHVASQIYEHTTEKVYLINDTDSEPDRRLIKEHFGKIIPIIP
                     SWELNIKKMASYSLVIVGYKHWNDIQKNPEKKLLFAGERQDFLPDINVPILILRG"
     gene            4725..6335
                     /locus_tag="PLF66_07520"
     CDS             4725..6335
                     /locus_tag="PLF66_07520"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012388630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HMZ63925.1"
                     /translation="MVKSKYIFITGGVSSSLGKGVTVAGLGCLLEARGYKVALQKMDP
                     YINIDPGTMSPYQHGEVFVTNDGAETDLDLGYYERFTTSQFSRKNSVTTGQIYHSVIE
                     RERKGDYLGRTVQVVPHITNEIRNRIQLLAKEQEPDFVIVEIGGTVGDIESVPFLEAI
                     RQMRYEHGQGHVLFIHLTLVPTISAAGEAKTKPTQHSVKELLALGIQPDILVCRIGNP
                     MTKEMKSKISLFCNVKEENVISASDITSSIYEVPKMYKDEKLDEVVLKMFNMEIGKSN
                     FGEWEKMVKRILNATKKVNIAIVGKYISLQDAYRSIYESLSHGGIANDAIVEFTKINP
                     EDLNKDNYKDLLKDVHGILVPGGFGERGIEGKVLAINYARTKKIPFFGICLGMQCAVI
                     EFGRNVVGLKNAHSTEFNPSTEHPVISLIEEQMEIEQMGGTMRLGAYPCLVKKGTLAY
                     SEYKSERISERHRHRFEFTLRYKEMFTKAGMDFSGYSPDEKLMEIVEIKDHPWFIGVQ
                     YHPEFQSKPINPHPLFAGFIRAAVKLLK"
     gene            6345..7199
                     /gene="kdsA"
                     /locus_tag="PLF66_07525"
     CDS             6345..7199
                     /gene="kdsA"
                     /locus_tag="PLF66_07525"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016548137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HMZ63926.1"
                     /translation="MSKRPTEREFLNGNTIGGNKPFFLIAGPCVMESRDLLDRVAGEM
                     IEICKKLGIFYIFKSSFDKANRSSVHSYRGPGLHEGIKNLEYIKSKYGIPVLTDIHEV
                     EQVEPLKDVIDVYQIPAFLSRQTDLLEAAAKTGKWVNVKKGQFLAPADCKHIKEKIRE
                     CGSEKYLVTERGASFGYNNLVFDLRVIPILHSLDIPIIFDGTHSAQLPGGAGNITGGQ
                     REFIPDLMTGAVSLGVEGLFMEVHPDPPSAKSDSTTQFYLNKAEKLITNLYKLDRLVK
                     TELVELES"
     gene            7259..8536
                     /locus_tag="PLF66_07530"
     CDS             7259..8536
                     /locus_tag="PLF66_07530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63927.1"
                     /translation="MQEDSALFESWEEPEYPKYSGYYSPTFANLDSLWYRTTTDSKYA
                     DGRDYLDSLWNRECDKIIFKYINKYGPYFSAFEIDILNEIEKVMNQKIDEKRINFFYY
                     IATKAHEIKEIENIWISKFEDECKKKVVCKLCKYEEFRIHQEPKIVRGSNANFIYCSN
                     CYKRTLMYKSFWNDEIEQIYIQLVNLVNNKVLVNCNLCGVNFVHSPGIFRNVMHDFVY
                     PNLFTTICGECFQKAYSDNWKSKKEILYHTLYKLFLYVGRIPTYEFYDYQYLFKDQES
                     ILQLFSFFIDLRSPEGFKKLDGSFYKTLINAGILKSNSRNKILGKVILSSDGHICFSN
                     LEKEIDDFLFERGLNHLKDTQYPESNLLTNWKIEIDEKIIFIEYILQTDNDYFSDLIY
                     KKKWIAKEYNIHLLCIYPKDDWKRKISECIDSI"
     gene            complement(8566..8742)
                     /locus_tag="PLF66_07535"
     CDS             complement(8566..8742)
                     /locus_tag="PLF66_07535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HMZ63928.1"
                     /translation="MKQIEMDGFSESAISRLESRTDFKISTLIDYISQLNLELEITVR
                     TRTPKKKEFVLVKA"
     gene            9090..9533
                     /locus_tag="PLF66_07540"
     CDS             9090..9533
                     /locus_tag="PLF66_07540"
                     /inference="COORDINATES: protein motif:HMM:NF026878.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin type 47 domain-containing
                     protein"
                     /protein_id="HMZ63929.1"
                     /translation="MKNGERLKDWEKDRFVELLWKVKGIRLFTCKSNYITKIGSLTGY
                     KVTENDLDWRGTGKTLDDALKEAFAQTGIKPDDFQPSGIEGLTETGKTVPVEWKVVGI
                     AEVNIDAPHTRSGPDVFHVGYKVYGSKKKVGHIFMDCLPYFREVK"
     gene            9536..9976
                     /locus_tag="PLF66_07545"
     CDS             9536..9976
                     /locus_tag="PLF66_07545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63930.1"
                     /translation="MVKNFLFKIANEHNFNLKTIPLEQSNFLRNDLYRKYKVENRGLF
                     LWDSYIPNSYLSNREDSWLGLDDFIQSKETIIFFDQKVEANFFELLSGQSIVSLLENY
                     KHDEYYLTNRNLDFLICCTDLGHLVAAGTARDWLEKQDLAVSSG"
     gene            10093..10296
                     /locus_tag="PLF66_07550"
     CDS             10093..10296
                     /locus_tag="PLF66_07550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63931.1"
                     /translation="MKTAIEYFDFNSGKISDCGDPLEIELSSRHLGWKGVLLEKGWSP
                     YFYPNNIYTNSFYFALALDSPLH"
     gene            10435..11007
                     /locus_tag="PLF66_07555"
     CDS             10435..11007
                     /locus_tag="PLF66_07555"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HMZ63932.1"
                     /translation="MLQCFDEKLPTTKLNFLNQYNVKDNTIMNIINLFWEEVKTNGSN
                     GKQYVEMLKKLLANYFIKNYSDYKDILNQKESVKIKAEDIEIVKSHILSNLGSEISID
                     TLAFHLSMSKFYFLKEFKKATGITPYQYILKIKLEEAKRFLQETEKSLVEISLELGFS
                     DQSHFTRTFTKQFGITPGVYKSNYLQINTN"
     gene            11073..11942
                     /locus_tag="PLF66_07560"
     CDS             11073..11942
                     /locus_tag="PLF66_07560"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HMZ63933.1"
                     /translation="MSKNVLITGVGGGFGKLIARVLTEDGYSIFGTMRDVNGRNKENK
                     IELEKLGIKVFEMDVTNTKSVDNVIDHIMSKVNLDVLINNAGVGVLGNQETFTPEDFQ
                     KLFDINVFGVQRVNRAMIPYFRKNGNGLIIYISSLLGRISVPFYGPYNASKWALEALA
                     ENYRIELSGFGIESCIVEPGGYPTTFIDNLIRPSDVDRKKQLGEFADFPEEFLKGFEH
                     GLASNPEQDPRNVAIAILELIKKPKGEKPFRTIVDKMGMGTYIEEYNQHLSKVTEGIY
                     AAFGISHLLTVKK"
     gene            12099..12488
                     /locus_tag="PLF66_07565"
     CDS             12099..12488
                     /locus_tag="PLF66_07565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63934.1"
                     /translation="MIKKITILVFILHSIFAYAQKTKISNPFDTFLSGVYSHDTDKLI
                     SSFSEDAIVIDVGRKIVGKKEIQKWAKSEIIDAGLVYKILSQNSKKENLQEYIIHISS
                     KSFTFKARYLIQTENEKIIKAVLEYAD"
     gene            complement(12634..14097)
                     /locus_tag="PLF66_07570"
     CDS             complement(12634..14097)
                     /locus_tag="PLF66_07570"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HMZ63935.1"
                     /translation="MKLYDVAIIGGGISGFTAALRLQKKGFNTLVIESHSQIGGCAGY
                     FTKKNFSFDVGATTLVDFIEGGVGGNFFQEIGLPIPEGEYLDYIAWLPDRKINLYRDN
                     TRWKEERLLKLGNSKNHIAFYELLDRITNVFWEATRKNIQIPIHRFSELKKAISAIGF
                     KNLGLLKYYNHTMLDVLKFFHLEKEKPLIGLLSMLIEDTVHSKIEEAPFINATLGTTI
                     RGAGLMRAKGGMKALWKILENHYNSLGGEIYKSNQVISFVKQNNLWEITTKKGNLQAK
                     KIISSLPLDNTFTISPEFIQKKLKPYIQKNEVYKGGAIVVFLGIPEKEIETESITHHQ
                     ILLDYDRELGNGNNMFISVSGKEDALSAPIGFRSVMISTHCKNLEWENLSKEEYESKK
                     KEIGDRLIDYARRVYPSLATNPVVYEIGTPRTYQKFTKRTSVGGFKQTLSNSNWKSVP
                     QDIGIENFCITGDNTWPGLGTVAGLVSGRIAAELSSH"
     gene            14402..15919
                     /locus_tag="PLF66_07575"
     CDS             14402..15919
                     /locus_tag="PLF66_07575"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HMZ63936.1"
                     /translation="MQKQIFTKMSFGKIISFFRQIVSFLKNPEDMHKLILKLAEESEE
                     EFVPILLNPFDPMLLVKDASILHLILKDVEFQNRSSFPIIDYIYFQFIKNLNSLENKN
                     PKIHTNMGGITFTNGEEWKRNRKIGLTVMNDPIFLEKSIEPILNSIDAMLLNLKEKKT
                     QFAISDELSLLGIDVVGKLVFNIDLELFSKEKRNKNQIDSYFLTSSVRVMLDAIQKYI
                     YYPFPAFLFSFFKTKDIRNLDRAVSNLALAGNTLLSKVKEDLQLGNSRSESSIAGILL
                     QKYPDLEDTEIQTLLMDLLGAGHDTTANLVIFTLSSILESNVLETSKELQKEIASIVE
                     ILPSFQKANENIDSKLILEKIEKNTPILSSILNESLRLFPLGAVFSRITFSKKEIHFN
                     IKDKEYILKPKSKLLVSPYVTGRLKKDWGRSANEFDPFRFLDKEKGNAFIPFGSGKRS
                     CIGARFGILEAKLILFRLFQIYKIEKGESFQKTDSILAFTLRAKNPIWVLAKANT"
     gene            complement(16040..16855)
                     /gene="ygiD"
                     /locus_tag="PLF66_07580"
     CDS             complement(16040..16855)
                     /gene="ygiD"
                     /locus_tag="PLF66_07580"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010570640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HMZ63937.1"
                     /translation="MNLKEFGNLLESKTEKKMPVAFIGHGNPMFAITENPYKTLWATF
                     ANTIPKPTAILCISAHWLTRGTGVTMATNPETIHDFGGFPDELFQVQYPAPGSPTYAR
                     MAADAIKSAEVQEDYKWGLDHGTWAVLRNMYPKADIPVFQMSIDYYKSLEFHFKLGEE
                     LRSLRSKGVLILGSGNVVHNLRQVRWQENAKPFDWALEFDQFVKNNIEEKNDKALLEY
                     KNLGEIASLAHPTNDHYLPLLYILGLREANDSYSFFNDSIDMGSMSMRSVVFG"
     gene            17099..17797
                     /locus_tag="PLF66_07585"
     CDS             17099..17797
                     /locus_tag="PLF66_07585"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HMZ63938.1"
                     /translation="MIRLLDTLKLKYTKRLFLSSGAISVRLPLSLNIAGKIYHLETNM
                     ISREYIFIKIYEEDFEFLPSVYSHIILFMNLDLFNIGKYELEGKLVSIDKIAGSRCGL
                     WIEYKTETESDRKTISQFVQNHYTPRYSVRFSVEIKTDHKTLRGEAINLSEKGIFIEA
                     ELGHLEENKMYSLILYLESDIIHVNAQVSWINKGKMYDKPNGYGLKFLPDKNAEKKIT
                     QYLNTLKKRSAILR"
     gene            complement(17799..18950)
                     /locus_tag="PLF66_07590"
     CDS             complement(17799..18950)
                     /locus_tag="PLF66_07590"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HMZ63939.1"
                     /translation="MIKKNTFSIYKMTLSNQNPKARSCYKTKQFKSRLFKKTYLVLIL
                     LLLNILQTPIFSDVQKKTRTLKVLVAGDVMFNWGLRETRQKRGEFAPVEELIPLFLEA
                     DIRMVNLETPVANSTDDMDESKSYVFNAKPEDISLLKKIKIDLVFLGNNHSMDYGKKG
                     LEETIENLNKEKILFAGIGKNIREAIKPNQISIRNKSVQIISVSNIGESRLFAGEGKP
                     GVAPLQINRLKTVSSDKNNQGKIQLLSVHWGVEYSPEPTKEQIKQGHELINSGFTAII
                     GHHPHIPQGIEKYKNGIIIYSLGNFLFGSRNQYLNHNIAVMLHFENNELIFCEVIPIF
                     GKFQDAEHIVRPLPYSEAEDFLHEYSILCQNLNTKLEIKNGRGYIYIKK"
     gene            19040..19426
                     /locus_tag="PLF66_07595"
     CDS             19040..19426
                     /locus_tag="PLF66_07595"
                     /inference="COORDINATES: protein motif:HMM:TIGR04452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04452 family lipoprotein"
                     /protein_id="HMZ63940.1"
                     /translation="MKKIIFSLATLLSISNCIVLDKLGLNLVKETVEGQAAINQILTG
                     ALIGAAASGSTTTFATSIATYNTKGLKHSKFYDKDDVDACADKVIIVANAVSNNQYTR
                     GLAVGDIACSPIREHKTLIDWPVPLF"
     gene            19594..19974
                     /locus_tag="PLF66_07600"
     CDS             19594..19974
                     /locus_tag="PLF66_07600"
                     /inference="COORDINATES: protein motif:HMM:TIGR04452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04452 family lipoprotein"
                     /protein_id="HMZ63941.1"
                     /translation="MKKIIFSLAVLVFVTNCVLIDSLGLNVTHATIKGSEAKNLILTN
                     ALAGAALSGAAGTSVATAIVTYNTKGIKDSKFYDKDDVDACANNVLAVNYAAVRSTGV
                     APGDLGCGSIREHKMIIDWPVPLL"
     gene            complement(20053..20898)
                     /locus_tag="PLF66_07605"
     CDS             complement(20053..20898)
                     /locus_tag="PLF66_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002764028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HMZ63942.1"
                     /translation="MIINHNLSAINSHRVLKFQNEEVSKNMEVLSSGMRINRASDDAS
                     GLAVSEKMRSQVSGLRQAERNTEDGISMIQTTEGYLTETNDILQRIRVLAIQASNGIY
                     TPQDRQMIQVEVSQLIDEIDRIASQAEFNKMNLLQGDFARGSRVASMWYHVGANMHQR
                     ERVYIATMTVRALELKRPDGTMLSLSTADLANDAIGTIDDALYKVNKQRANLGAYQNR
                     LEHASKGLMVAYENIQASESRIRDADMAEESVAFTKNQILVQSGTAMMAQANVRPQSV
                     LQLLR"
     gene            complement(21266..24283)
                     /locus_tag="PLF66_07610"
     CDS             complement(21266..24283)
                     /locus_tag="PLF66_07610"
                     /inference="COORDINATES: protein motif:HMM:NF015859.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE domain-containing protein"
                     /protein_id="HMZ63943.1"
                     /translation="MKQNLHRSDQIPWILLTIFFSLTCIVSYFLRESSISTNALEFSF
                     LCNEIKSKIITRLESQAQILRDGAAYLQSRDSISRKDWKTYIDESEAIQSLPGILGVG
                     VTILIPKTKLQEHIESVRREGFPNYKVFPEGEREIYSSIIYLEPFVGRNLRAFGYDMY
                     SENIRRKALEFSRDYHTPGLTGKVILVQETNEDVQPGTLLMYPFYKKNMPIATIEERR
                     KAIQGWVYSPYRMKDLLEGILSNQNSGENLRLEIFESEEIKQGNLLYDSGNIPQQNFN
                     LKISQKVIPISIFGSTWSLRFTDLRPKANFFYFNRSYIVFISGVIVSFLLFFLTKISI
                     NTEKKAIALADSMTLALKESREWFRMILNSTADPIFGLDLNGNCSFCNFACVEILGYT
                     SIDDLLGKNMHHFLKHSDSSGKPIQYENCKILTAHNKGEKVHSDSELFKKADNTFFPV
                     EYWSYPILINGKIIGSVVNFLDITERKKLNKELQEKIQQAELANRAKSEFLANMSHEI
                     RTPLNGVIGFIDLLSKTEMNSNQRQYMKIISQSSNSLLDLINDVLDFSKIEAGKIDLN
                     IEPVDIYELTAIAIDVIKFKAHQKNIELIYNINPDIPKYIWTDMVRLRQILINLLSNA
                     IKFIDKGEIELKIDFNFPETRKDIIILHFSVRDTGIGIDPKNSKRIFQAFTQEDSSTT
                     RKYGGTGLGLTISNRILNTMNSQLELESELGKGSRFYFSIECRFQKDKLIEPMETNVK
                     QAIILETNRSNAGVIQKFLNYFQIESEVTFNLEDAKILLSRKKYDLAIVDTNLPGESS
                     IEFIKKYIEPIPSILLYDALEEGDFASMPEDKHILSKRMKPLKPYDLFSEIINLTTKD
                     KKVKETNKIETESSNQELSFSKVLVVDDDSINLLLAKTILKSILPQSIIMQAENGKDA
                     IEIFKTENPTLIFMDIQMPEMSGLEATREIRKLEQERPCTIIALTAGAQKGDFEKCIQ
                     AGMNDYITKPVVKGTIEKAIQKWSKILQATTPK"
     gene            complement(24448..27618)
                     /locus_tag="PLF66_07615"
     CDS             complement(24448..27618)
                     /locus_tag="PLF66_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020775930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HMZ63944.1"
                     /translation="MSKLEKGFAGKLAEQFINSRLTPVIAIVSILLGLMSVYLTPKEE
                     EPQISVPMIDIQIFAPGFEAKEVERKVTEKVERAVWGLDGVEYVYSATKAHGSIITVR
                     FKVGEPTEPSLVKVHHKLMEIRQDLPSNVLPPKISSFTIDDVPFLTLSFSSSERDDYD
                     LRKKIAPLARELSSTPDLSRVELLGGRRESIRIILDPVKASNQGVSVLDVANTLKMSD
                     AYFPMGKTYSKEKEFQVEVGGRLSSSEEVGNLAIGQRGGSIVRLKDIAKIQLAAEERN
                     RLSILYDKDNGTEPKNAVSIVFAKRKGTNVVVLAKDLMERAETFTKDLPADIKMSIVR
                     NYGTTAEEKSFELIEHLLIATISVTVLIALVMGLRSAIVVSIAIPVTLALTLAIYYFM
                     GYTLNRVTLFALIFSIGILVDDAIVVVENVERHLHLDPESGILKATINAVSEVGNPTI
                     LATFTVIAAILPMAFVQGLMGPYMKPIPVGASLAMILSLFVAFIVTPWASVRLLKQTV
                     THHSEETPKESFLDKLYISFSEMLLGTKKAAFLFGIISIILLLSSFSFIYFKWVKVKM
                     LPFDNKNEFQITIDYPPDTPLEKSVEWSKELSAKLLQEKEVKKIQIYAGESAPFSFSG
                     MVKHTFLRKEDYMNDLQILLSDKGKRKESSHKIIERLRPIIQEFGKEKNAITKVLEIP
                     PGPPVMATMVAEVYGPTEEIRRQVAKEIYSIFEKEKSVVDLDYSWRESKTRKVYDYDQ
                     IKGGILGAKSMQVVGVGSYIFTESNLASLAEADAPEETNVVLSVDNKVRSSQIPFQGS
                     KIASFESGVIPVDSLLKNPKLISNEVLHRKNLKPVSYVLSELSGAEEAPVYGMLKLAP
                     NINYPTQTADVPWNTKSPVIKWDGEWFITYEVFRDLGGAFGVVMILIYILVLGWFKSY
                     TVPLIIMTPIPISLIGILPGHAMLGAYFTATSMIGFIAGAGIIVRNSIILVDFIEEQL
                     KEGQPLKEAVISAGVIRFRPMLLTAAAVVVGSFIMLFDPIFEGLAISLMFGEVAATAL
                     SRFAVPVLYYWFVGKSRVKLFSEANS"
     gene            complement(27611..29038)
                     /locus_tag="PLF66_07620"
     CDS             complement(27611..29038)
                     /locus_tag="PLF66_07620"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HMZ63945.1"
                     /translation="MKSNATKLILIGLFFSSYFGLLAEDSNVIEFNSIWEKIKQNSHS
                     QKALSLESRAAKISSNKASKHWLPRVYADARNFTTNDPALNFFSTLGQRSAAASDFST
                     KSVRTQPSNFIDTNNQLYTTPNYNTLNLFAPDTLNYPGTNTYQRGTLGIDFSIYEGGS
                     KKAVAKAYEKQSEGKQLENKFVTLNEYANFAAMYGTLIALNEQQEKINSLEKVLTSIL
                     NRYQLGNRGNPVGYSGGIALKSLKNRIDGIKEETTARIDSIKSSIETAAQTLPEKWTL
                     KSQSAMSFANEYLKLQAPDKSYMAKAMKSYAESATEQAEAEKARVLPKVGLYGESYLY
                     RGDRATANSFNAGFYIQMNLYSPGDLDSIEQAKLNSKAMQERAEETIRNEEAKSKSLV
                     QIEKALNKNLELIQENTKLMEEQITVSQQLFSSGAINAIQLAEVLSRRADIILAKSTL
                     ESEYLKTRAGILTLSETNIEGLKYE"
     gene            29145..29366
                     /locus_tag="PLF66_07625"
     CDS             29145..29366
                     /locus_tag="PLF66_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002975163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HMZ63946.1"
                     /translation="MYLAKTDKWYLERVIWLLAGLFTLASLGLGYFVSPYWFILTLLV
                     GINQIVLSLTGFCPMTVILDKAGCHSRIS"
     gene            29580..31634
                     /locus_tag="PLF66_07630"
     CDS             29580..31634
                     /locus_tag="PLF66_07630"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HMZ63947.1"
                     /translation="MVVFIRNILYFSSIFGFQIVFWEFLKRFDYSIAILILFFAFDLF
                     IIIFTINYFKIFLISNDAPLLPNLLLRDEIIWEINSKGDQFLNICARDERLLNYTKEE
                     WLKPNFLEDFIYPLDKEITFKNFQDMIQIKSECNFSFRFYTKNRNIVWFEAKLEQIKN
                     QNGIEHFYGTLTDITEQKKKESLLQFHSDILNKMSEGVFLLRTSDAKIIYTNPSFDTL
                     FGYKNGELIGKHVSILNSSDTKPSREVADEINANLYKDGFWRGEVHNQKKNGESFWSE
                     VNVSEYIHPEYGLVWISVHNDITQRKKMEEDLVKAKQDADKANQAKTDFLVRMSHEIR
                     TPMNGILGITSLLVESDLSPEKKGYAELAVRGVEVLESLVLNIMDFSKIESGTIHIEK
                     TECNLSEMIHSIFSLFELQAKEKGLDFNLEMDIKFPQIVICDMHKIRQILINLISNAI
                     KFTNKGYVKIKLRVIEDLGDKKNLEILVQDSGIGIKEEQKIHVFESFFQADSSMTRKY
                     GGSGLGLSISKKLVNILGGNLSFESKEGIGSTFCLSMIVEIPKERVKNSTIEKVSLHK
                     ENPKILIADDDDLNAMVAIQILKSKNIHNVTHVYSGLDALKEIETSDYDLIFMDLRMP
                     SMDGIETTVLIRRISDIPIVAITGEVIEGEREKCLAAGMNDFYTKPMSASDFMKIIHE
                     WT"
     gene            31849..33048
                     /locus_tag="PLF66_07635"
     CDS             31849..33048
                     /locus_tag="PLF66_07635"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002763082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006102 - isocitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HMZ63948.1"
                     /translation="MSKIKVKTPLVEMDGDEMTRVIWKEIKDRFIHPFLDIELEYYDL
                     SVTYRDKTDDKVTTDSAQATLKHGVAVKCATITPNADRVKEYSLKKEWPSPNGTIRSI
                     LDGTVFRKPIIINNIPAAVRSWKKPISIGRHAYGDIYRDVELQIPSAGKVELVYSDTS
                     GAEKQRVLVHEYKGPGVVMGMHNLDKSIISFAKSCFNYALSEKIDIWFATKDTISKKY
                     HARFRAIFDEMAAEKEAELKAAGISYSYFLIDDAVAQIMKHQGGMLWALMNYDGDVMS
                     DMVASGFGSLGLMTSVLVSPEGKYEYEAAHGTVTRHYRKYQKGETTSTNSVASIFAWT
                     GALAKRGELDGTPEVTAFAQRLEKAVIDTIEAGEMTKDLISLSTAPVKKELDTFQFME
                     AVAKRLK"
     gene            complement(33237..34088)
                     /locus_tag="PLF66_07640"
     CDS             complement(33237..34088)
                     /locus_tag="PLF66_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010569068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HMZ63949.1"
                     /translation="MKVFVYGATGSIGSNVVNGLLERGHEVFAGTRHPEKGKKATNLN
                     WVIADAEKPKEGVEVLEKVDAAFFISPPGLTNQYDILNPWLEKAKSSKIKKFVLMTAM
                     GVEHAPPEAPFRKLEIALEQSGLSYNIIRPNWFMQNFQTYWISGILSDKKIYFPGGDA
                     KVSFIHAKDIASTAITLLLTDEFKNQAFALTGKEAITHSDVASKLSKATGLSISYVDI
                     TPEDFKKALLGAGLKEDYADFMVFIAGNLKQGHSSPIIDSIKKITGKDPISFDEYAAE
                     NKKVWLG"
     gene            34301..35203
                     /locus_tag="PLF66_07645"
     CDS             34301..35203
                     /locus_tag="PLF66_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008589311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HMZ63950.1"
                     /translation="MDILSEIINNANWKGDLLSRNSFSKPWGLIFPCEKSAGFHIVTQ
                     GKCYIRYKNKSILMSKGDIAFIARGFTHELASDPNQKAMDIKKFRQLIQAQMETQKII
                     TSILSVRYEIKNTPMHPFFLELPDVLFIKSEEIPTHHPLQTILILISQELDRGIGSDV
                     LLEKLSDILLYYVIRHWMETNPSHSPGWMSALKDEKILSALEAIHLNPSHAWTLDLLA
                     NSVGVSRATLANKFRDTLGSTPMDYLAKLRIEKGKNMLIEKKATIEEVARSVGYSSAF
                     AFSKAYKRITGYSPKTSRDTAAVV"
     gene            complement(35205..36080)
                     /locus_tag="PLF66_07650"
     CDS             complement(35205..36080)
                     /locus_tag="PLF66_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016546969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HMZ63951.1"
                     /translation="MAIQSKRKKENILGNFYVDSTCIDCETCRILAPEIFKKVGNGSI
                     VYFQPTTNDSIERGLQALVACPTYSIGLETMLPELKEIRLSFPKNIADTVYYNGYHSE
                     KSYGAASYFIKREKGNILIDSPRFAMTLVENFKKLGGIDYIFLTHQDDIADQQKYADY
                     FGAKRILHKDDFTKQLSSVEIPIEGEEIFHLDEDIKIIPVPGHTEGHCVLLYKDSFLF
                     TGDHLAYSPTKGHLHAFRNACWYSWEKQIDSMKRLNEFTFESILPGHGRSFSSDKTTM
                     KSQLSKCIEWMESVK"
     gene            36788..37384
                     /locus_tag="PLF66_07655"
     CDS             36788..37384
                     /locus_tag="PLF66_07655"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020779569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HMZ63952.1"
                     /translation="MPQVTSQAPDFTAEAVIGSEFKNLKLSDYKGKWVVLFFYPLDFT
                     FVCPTEIIEYDTKLDEFKKLGAEVIGVSIDSKFSHLAWKQKPRKEGGIGDIKYPLVAD
                     ITKQISKDFGVLIDSGDDAGVALRGTFIIDPKGVIRQATVNDLPVGRNIDEAIRLIKA
                     FQHNEKYGEVCPANWNEGAKTMTPDVNKSKEYFSAVNK"
     gene            37502..38923
                     /gene="sufB"
                     /locus_tag="PLF66_07660"
     CDS             37502..38923
                     /gene="sufB"
                     /locus_tag="PLF66_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015678897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HMZ63953.1"
                     /translation="MTTITTSIQEENERYYRADNFPKGLTRKVVESISHIKNEPGWLT
                     EFRLQAFEVYEQKPMPTWGFFPEFNVNIDSYTHYVGSNQKKKKSWEDVDPEVLRSFEK
                     LGIPEHERKYLAGIEAMNDSETVYANVKKELTDLGIIFCDIDTAIKEYPELVRKYLGT
                     VVTIGDNKFSALNSCVFSGGSFAYCPKGVKTPMPLQAYFKVTAASSGQYERTLLIADE
                     GAEIEYSEGCSSVQDKGTNFHTAVVELIAHKNAKINYTTIQNWKKNMYNWTVKRGLCH
                     ESAHITWTDCNIGANTIKYPGIVLLGDNSTGSVLSLAFAGHGQVQDTGARIIHVGKNT
                     RSNVLAKGVSLDGGVNSYRGLVKFEPSSKDAFSHIKCDGLMMDDHSSSHAYPYNDISG
                     EHGSLNYEATVSKIDEDQLFYLQSRGLSEDDAKLLIVNGFCESIVKELNVEYSVEMTR
                     LIRMILEDGKVIAEAPKVEKIAQ"
     gene            38920..39942
                     /gene="rlmN"
                     /locus_tag="PLF66_07665"
     CDS             38920..39942
                     /gene="rlmN"
                     /locus_tag="PLF66_07665"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012388354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HMZ63954.1"
                     /translation="MKTILKGMTPAQLEDFVSSLGEPKFRGKQIFQGIYKDRLSSFEE
                     FTTLPKKLIAKLNEVAELPNLKVRRHLTSKDGTHKMTFEVEEGKEVESVWIPSGDDGR
                     KTICISSQVGCTLACKFCATGLLDFKGNLKTWQIIDQVLQVEKIVGDRATNIVFMGMG
                     EPMHNYFSVINAAHLLHHPEGFNLGAKRITISTAGVITGIDRFVNNQEPFNFAISLNH
                     PNPEARKTVMGIDEKYPLEKLLIAARNFTKKLNRKITFEYVMIPGVNMGKENARRLVK
                     IARSVNCKINLIPLNTEFNGWSRPTEEEVEHFKELIKPAGVPIMNRRSPGKDIHGACG
                     MLALRG"
     gene            complement(39931..40671)
                     /locus_tag="PLF66_07670"
     CDS             complement(39931..40671)
                     /locus_tag="PLF66_07670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HMZ63955.1"
                     /translation="MNIIKITIFQKNINTDITRDQKQKLISQKSDFLILPRFFPTLAK
                     VSEKSSLHNQKIYIDKLLEISEYYKGIIIGGTILREVNGKIYESCPIVQDINVIDWYD
                     ARIPEQSGDIPISAGESESIFILGGVRFAILIGNESKNEAYLSHIQEEKVELLLNTSM
                     IYESETEPSEYQKDLDLFAEYSKTYSLNIVRSSGIGRLFNNKTLSGRSFYSSNTGIKW
                     KVASFENQNEIIKTININVLETMHSLTS"
     gene            complement(40699..42525)
                     /locus_tag="PLF66_07675"
     CDS             complement(40699..42525)
                     /locus_tag="PLF66_07675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63956.1"
                     /translation="MAEFDKTKKAIGVGGMAENERNEMFNKFKSAGGKVVKDKEDKPQ
                     QQPKNTQRMKAVGGKDDKSKSSSGDKKFSPGMYRGIGASDDDDDSSEVSISKAQIGAM
                     DAEMSNFMNRLIIKFKCWANNVTTFGNKDLLPKFMSELRVEFQQALLEFKIAGSDILG
                     NPQLGPKVTKELDRISPIYVELIGRAHKIYDTVEMNDLFADYSSNPDIPVPINRVAKP
                     VYSIFKKLYYLYPYQATYRKAVLTAYEVLQRIENKPALIYATKKKKISSEISVVFDKI
                     FEKLYYIIIRNENKNIPMVSLYMENLLNILESEKPGKRKAGENLPLDAGGEQSTEEAQ
                     AEEKKENPNEKKGENQELAYGRKLMSEVSIEQLRRKYDPRNELIEIPDTDKALLTYLF
                     FKEFDYEYSFVLTTKKITIKPSNVNGTKVDNKQKLLNVYELTRSCMEQFKIYIDVFKE
                     QAMHKANPGTNYIEASKKTTGLEQKRSQQSRNVRVTIKDYIEKSYDSFNMLIEDMKGP
                     AEIVGNMEEVIVFDSIESKKRLHKKQVKQCIMEAYCYSYAFATRLTEGGDLYGGVIEL
                     TPEEMLASFGKDLSGEDLSINNLMEGGAKKTESSGPKDMGDI"
     gene            42798..43955
                     /locus_tag="PLF66_07680"
     CDS             42798..43955
                     /locus_tag="PLF66_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011669869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HMZ63957.1"
                     /translation="MSIYSQAKKNLNQISVGEFWTAKQRQSHPIHYAVSYRAAFKPEL
                     PSFFIREYLGNKKRVILDPFGGRGTTAIQANLDGHYAIHNDINPLSVFLAKSRQIVPT
                     LSEIQEKLSSLDLKKKVSDSEMDEKLEPFFHKDTLREIKNLKLIGKEDKSHTMSYILL
                     TALSRLHGHSKGFFSVYTFPQFSISPESQRRNNLKRNQKPEYRDIKSRILAKAKRDLS
                     KSLPPFYHEFSKFNQYTNHNSEQMTNIGTSTVDFVITSPPFLDKVNYKEDNWMKCWFM
                     DIDSECMDQISILHSVDGWSKFIKASLKECSRVMKKNAHLVMEVGDIQNGKSVVNLDD
                     VVIQASAGTGLSWEKTYINSQKFTKLSNCWNVSNNEKGTNSNRCVVFRNVK"
     gene            complement(43956..45758)
                     /locus_tag="PLF66_07685"
     CDS             complement(43956..45758)
                     /locus_tag="PLF66_07685"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HMZ63958.1"
                     /translation="MDLPVCKVRTSLYLFFLSAFFIMESCNLNSKTQLKFMQSLTGLS
                     SGSSATTGTTTSTVAAPSFTNLLNKGLLETGFVIGAATGTISKVEISIDSGTYSSASG
                     TNTWSYQLPTGSSIWKEGSSHTISARSVDAAGNTSSITSIIVRKGKNKDINGDGYSDV
                     VVGATGYSGGLNKGAIYIFYSSGNSGVTATSATSASKTISGQTNGDKFGYSIALGDVN
                     GDGYADIAVGAIAAVGNKGVTYIFLSNGSNGISANSAAAADASITGANSNDTFGSAVS
                     IGDINGDGYGDLVIAANYYPSSSNNGRVYIFHSNGSSGITATGVGSANSNITGPSNFD
                     NFGTFISTGDVNGDGYADLAAGANGYSGGSLLGAAFVFHSSGNSGITITNPNSASTKI
                     IGGASGDYIGTSICLGDIDGDGYADLIVGAIGTTGNKGMVYIFKSTGASGITATNVSS
                     ANTSILGNSPNDSFGFSVSIADFNGDGYGDLAASANGYSGGANKGAVYLFTSSSSGIS
                     VTNVNSANTTISGNASSDIFGYFTSVGDLTGDGYADLVIAAKGYNAGTNTGAIYIYHS
                     SGNSGITATTSANASTSILGSAANDLFGTALSVF"
     gene            46539..47333
                     /gene="flgG"
                     /locus_tag="PLF66_07690"
     CDS             46539..47333
                     /gene="flgG"
                     /locus_tag="PLF66_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017851095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HMZ63959.1"
                     /translation="MRSLWTAATGMNAQQFHIDTISNNLANVNTTGFKKNRADFEDLV
                     YQHQVLAGTPATAVSEIPTGVNVGHGVRAAASQKLFEIGSFQATGNKLDMAITGEMGF
                     FKIQMPDGTFAYTRDGSFKIDSNQQVVTSNGYLFEPPIVLPENAILNTLQVAEGGEVT
                     VKIGNDIRPTVVGQIELYRFVNPAGLQAIGKNLFKETVASGPEIAGVPGTEGMGEILQ
                     GFLEMSNVKIVEEMVAMIVAQRAYESNSKAIQTSDNMLATAISLKR"
     gene            47347..48264
                     /gene="flgA"
                     /locus_tag="PLF66_07695"
     CDS             47347..48264
                     /gene="flgA"
                     /locus_tag="PLF66_07695"
                     /inference="COORDINATES: protein motif:HMM:NF024544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HMZ63960.1"
                     /translation="MFKGHRIKVSIQTFLVPFLFLFISSIQANGLYLKPRTIIEKYPI
                     KLSDVARLHKDIKDRVLFDSLEDIKILTIDELKDSINDPNISFYGKECLIIPLNKKYS
                     KEEILESLEKEFFSKLGIQSTEAKIHYLGEEETFPSSGVELRWNNLPKRLTPGQKIFS
                     LDFYVEREKIYSKKLKFLVETPLTALVASHDIARNAKLTKSDFMTKTFFSADAIADGF
                     NHDITGYTAIVAIAEGSVIRKKQVREIHLVEKGMEVDIVFMKGSLFVRGKGVAKNSAN
                     EGELVKVVNSSSNHTVSGRAMDKGVVVIE"
     gene            48261..49010
                     /locus_tag="PLF66_07700"
     CDS             48261..49010
                     /locus_tag="PLF66_07700"
                     /inference="COORDINATES: protein motif:HMM:NF014195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HMZ63961.1"
                     /translation="MKNRYNHFKKQNATGFENYKRAAIHRFAMVKNLCLLFIIFLLSV
                     VIPLGSQSLWVDKNPYANGQDIRLGSVIRVLIKDGMKGDYVYEGGKDDSFVIRSHPDK
                     KIVDEMKGFTADRSFAHRQNGKSKSTAKIIGAMSVLVTQVDPVSGLLTLAGTREVGFD
                     NAKNSLKLSGIISPLDVRDDKTVTSDMVANLKIEYATAPNKESLNEPDIQLKTKKNAN
                     GTETIQKAELSNEEKQKIILKNMKRLLGESQ"
     gene            49007..50179
                     /locus_tag="PLF66_07705"
     CDS             49007..50179
                     /locus_tag="PLF66_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000838053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HMZ63962.1"
                     /translation="MKANKSLKNTWLVRLRELGYFPFNSILVLSLFLFQIQSIFSVEV
                     RLKDISRIQGMRDNQLTGYGIVTGLSGTGDTKSAVTNEAIKNYLKNQGLGNAKGANIT
                     RNIASVLITAIIPSHARVGDKIDVVVSSIGDAKTLEGGVLMQSPLKGANNEIVAVASG
                     VLSFGGKDNNRSAQPYNATSYLYGNTANVFTGTSHAKNSPKTVGVILGGAIVEKEIKG
                     DFFQRTNKEDQENRYRIILENQDFATLNSVRQQISDKIKVETTAVSPTEIEIAIPKEQ
                     DPVSILAQLENLTVTPDGKAKVVINERTGTIVMGANVTVDEVAISKQGINVIISNKKK
                     DSDDTKVELISMIGEGTRVSDIVDALNKIGASTKDIIAILEGMKKAGALHAEVIVQ"
     gene            50176..50640
                     /locus_tag="PLF66_07710"
     CDS             50176..50640
                     /locus_tag="PLF66_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017214432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod-binding protein"
                     /protein_id="HMZ63963.1"
                     /translation="MNDIQNTIQFNRLNFGQKTMDAVQRIDANQPKSSLSFEETLRAE
                     INPNIQGKVSSSSIRLPHNIKKEIESDEYRKKLFDASVEFESIFVKMMLSQMKKSVQK
                     SGMIDGGYAEEIFEDMLYDEYSKKISQNESLGIAEEIYRTLSQSLPKVDIKG"
BASE COUNT        16639 a         8282 c         9034 g        16779 t
ORIGIN      
        1 ccgggctgta ttctattcga ttcttcatag cattgaaaac gtgcatcaca agaccaatag
       61 aacacggcta cagagaatat acaaaatata attcgattca tatatttaga attctcattt
      121 tattttgtat cctcgattac aatgcatcag gtttaatagg tgatttattt ttacacttct
      181 tagcttcttc aatggcagcg agacatccta ataaaatcag gtctttcaat ttatcaaata
      241 catctgcgct gagtataaaa ttatctttac aaaaaaattg tttattgatt ctagattttg
      301 attcacaaat agatttctcc gagttatcac aactatcgat taaaaataaa agtaaaataa
      361 cattaattat taaattgcct gacatatata tatatgctcc gactttccat tgataaataa
      421 aatttgataa tatttaagtg tttaactcag aaactgatag gtcaatatct taaaatccaa
      481 cgagcgaatt tctactagga aatatgtcat agcatagctt tgcgcctctg catcattgct
      541 tgaaactcgt attcatgata cactaaagct cccctaaagt taggactaaa cttcccttaa
      601 agatagatat ggagataaat ttaggataag tttaatagat gtttacccct aagtttaaga
      661 gatgtttggt gaaagcaaag ccaaacctct ctgtgcgctt cgtggtttta aaaaaagctg
      721 atagtaaaag ctattaaacc acgaagacca cgaaggaaca cgaagttagg ttaggagtaa
      781 aaaggaacac cctctgtgct cttcgtgcac ttcgtggtta aaagaggact gatagcaaaa
      841 gagattcaac cacgaagacc acgaagaatc acgaagttag gttaggagta aaaaggaaca
      901 ccctctgtgc tcttcgtgct cttcgtgcac ttcgtggtta aaagaggact gatagcaaaa
      961 gctattcaac cacgaagacc acgaaggaac acgaagttag gttagaagta aaaaggaaca
     1021 ccctctgtgc ttattccacc cttcgctcca agtattcaca accagcgctt tcgcatgtgc
     1081 ctcttcgtgg ttttgaaaaa agctgatagt aaaagctatt caactatgaa ggacacgaag
     1141 aatcacgaag ttaggttagg agtaaaaagg aacaccctct gtgctcttcg tgctcttcgt
     1201 gcacttcgtg gttaaaagag gactgatagc aaaagctatt caaccacgaa gaccacgaag
     1261 gaacacgaag ttaggttaga agtaaaaagg aaaaccattc gtgtcgatcc gtgtttatct
     1321 taaaaggtct tttaaaacgc atcgttatag tcaggcataa cgacttttaa ggatctatcc
     1381 tgaccgacag ttacaatgcc agcctttgct aaaacagtta agtagcgttc catgaagaaa
     1441 tcaaatggat cggattcatc tttatcatag atttgcaaag agctttctac cataaagagt
     1501 tttgcaaatt ttaaatctcc tgtgaagaag tagagaaagt ttttagaatt gggatttggc
     1561 atttgaggga ctaacaaaga tttgccgttg tctagggaga gaaacaattt atgatctaga
     1621 atttgtaagt tactaattgt tccactgagc ttgggaggag gaaagattgc aaaaggttct
     1681 acaaagattt gatttccttt tacaacaaga cctcttcctt ctggaatcgg aagaagcttt
     1741 tctagattta atcccacagc gcctaagagt gattttgcgt aggagtttcc aagtgcatga
     1801 atttcttctg ctgtgattac aattttttct tttttctcat cgagtgaaat ttttccattt
     1861 agttcaaagt cgagccagat gaagaaatga agagtgcctg taagtttaat tttatctaca
     1921 tttttttctg gaaattctaa ttcctttagt ttaagaggag agtcagtata attaaaaata
     1981 ttatctctca taattacttt taaagtagag ctatcgattg ttacatctgc ttttactact
     2041 tggattttaa acgcatttac atcgtctaag tttacaactt tcagaggttc aacgggaatg
     2101 atttctgctt gtaggttttt taaaagtaaa atgatatttt ctttgaaacg aaagcttaca
     2161 ttgtcggctg atagaaaact tcctacttta gaagaagatt gaataggatt tggtttttga
     2221 gtttcgtata aattgagtct tggtttagca gaagtgatga tataattttc aattctcaga
     2281 aaaacagtaa atccaagtaa aagtagcaga ataaaatttt ttaagaggct agccaagatt
     2341 aacctcttaa aattaagata ggaacattga tatcaggaag gaaatcttgt ctttctcccg
     2401 caaaaagtaa tttcttctct ggatttttct gaatatcatt ccagtgttta taaccaacaa
     2461 tcacaagaga ataggaagcc atttttttta tgtttaattc ccatgagggg ataatcggga
     2521 ttatcttccc gaaatgttct ttgataagtc ttctatctgg ttcagaatct gtgtcattga
     2581 ttaaataaac cttttctgtt gtatgttcat aaatctggct tgcaacatgt agaagtgaat
     2641 ttacttcaat aggatctgaa taaaaaactg ctactgattt aatatcattt atatttcgat
     2701 ctaggaaaac accaactgtg cagtccagtt cagatagaac actttctact tttccgccta
     2761 gaacgttatc tccaaagact ctcttagcag cacccattaa aacaagtgta gattgttctt
     2821 ttcgaacggt tgcagatatt tcctttggaa tttcgtctga tactttaaat ataggtttta
     2881 attttagttt tttttctttt gctatagact caagtggagc aaagaaattt gcatctatac
     2941 ttgtttcgct agtgatagaa atattttctg gaacaggaga gaggtgcagg gcagctacag
     3001 ttccattttt accgctgatt ccactcgcca attttaaaag agaaataccc gcactcgttg
     3061 gaccaaaagt aattaaaatt ggagacgaac ttccagttgg aattacttcc ttcttctctg
     3121 actcttctgt ttttacggaa agttttaaaa gaggtcctgt gataaatgta gtgactaacg
     3181 ccattaaaac catcattaca aatatagtag gggagataat tcctagatca tagccaatat
     3241 ttaaaacaat tagctccatg agtccacggg tattcattaa aacaccaatt gcaaaagaat
     3301 ctctccatga catacctgta aaacgggatg caaacatact ccctacaagc tttccaataa
     3361 ttgcaactat aatgattaaa agacagacgg cccacagatg tagatcattt aataatccaa
     3421 tttttgttct aattccagtg aaggcaaaga agagaggtag taaaaagatt gtgctaaaat
     3481 cttcaaattt ttcagtgaga acatttctga gtttggtatt agctggcatt acaactccag
     3541 ctaaaaaggc tccaaacaaa gcatgaattc caattccttc tgcaaaaata gcagatgcaa
     3601 ttacaattaa aagaagtgcg gttaagactc ccttgcctat gatttctttt gtgatgtaga
     3661 ctttgctcat tcgattgaga agtggctgaa tcaaaaaaat cataaccgca ataaagaaaa
     3721 atgaaataac cagagtaatg atggcagtgt ataatgttcc tgcatttaca atcgcgatga
     3781 tgatggcaag aatacaccaa gcgctaatat catccacagc cgcgcaggta atcgccatta
     3841 tccccaaggg agatttggtg agtcctcttt cttggatgat tctagcaaga acaggaaagg
     3901 cagtaatact cattgcaatt cccataaaaa gtccaaaaga aagaaagctc actccttgtg
     3961 gggcgaattc atcaaataga taaagtgaaa gtaagacacc tagagtatag ggaaaaataa
     4021 tacttgtatg actgatgaat atagcggatg acgctttctt ttttaggacg gacacatcta
     4081 attccattcc gattataaac ataaaaatca aaagaccaat ttgacttaaa atttttagat
     4141 agccgaaaga ttcttttggg aaaaggattt gtgaaaattc aggaaataag aaaccaataa
     4201 aggagggacc gagtgcaata ccggcaaaga tttcaccaat aacggaaggt tgtcctagtt
     4261 ttttaaatat cattgaaaaa aatcgcgcaa agagtagaac tactacaact tgtatcaaaa
     4321 ctacgctgag tggatctttt aaatttttct taatttcttt atgaaatgat tcaagaatgg
     4381 aatcattcga agcagcagtt tccgcatcgt tttttttcat ctgctctaat tttttgggat
     4441 cagttatttc tttgtgtgat ttgtatctgt ctcccttgat aaaaacaagt gtcattacaa
     4501 gagcaaaaac tactcccatc agaatgtatg ctattggttc atattttttc ataataacta
     4561 tgtaaaaaaa ctgcttttct ttataaaatt gactttatta attcctacct tatatagtca
     4621 tcattaaaaa atagtttcaa agctccttta cttaaagatt ttaattgaag aggaacagaa
     4681 attgaatgta agattaaaaa ctaaaaaaat aaaaggtgtg agatttggtt aagtcaaaat
     4741 atatatttat aacgggtgga gtttcttctt ccttgggaaa aggtgttaca gtagcaggtc
     4801 ttggttgttt gctagaggct cgtggttata aagtggcact gcaaaaaatg gatccgtata
     4861 tcaatattga tcccggaaca atgagtcctt atcaacatgg tgaggtcttt gttacaaatg
     4921 atggagccga aacagattta gatttaggct attatgaaag atttacaact tcccagtttt
     4981 ctagaaaaaa ctcagttaca acgggtcaaa tttaccactc agtcattgag agagaaagaa
     5041 agggagatta tctaggtaga actgttcaag tagtacctca tattacaaat gaaatcagaa
     5101 atagaattca attactcgcg aaagaacaag aacctgattt tgtaattgta gaaattggtg
     5161 gaacagttgg ggatatagag tcagttccgt ttctagaagc aatccgacaa atgagatatg
     5221 agcatggaca aggacatgtt ttatttattc acttgacgtt agttcctaca atctcagcag
     5281 caggtgaggc aaagacaaag ccaacacagc attctgtaaa agaattactc gcacttggaa
     5341 ttcagcccga tattttagtg tgtagaattg gaaatcctat gacaaaagaa atgaaatcta
     5401 agatttctct gttttgtaat gtcaaagaag aaaatgtaat ttctgcaagt gacattacat
     5461 cctccatcta cgaagttcca aaaatgtata aagatgaaaa gttagacgaa gtagttttaa
     5521 aaatgtttaa catggaaatc ggcaaatcta attttggcga atgggaaaag atggtgaagc
     5581 gtatcctaaa cgctactaaa aaagtgaaca ttgccattgt tggaaaatac attagcttac
     5641 aagacgccta tcgctctatt tatgaatctt tatctcatgg tggaattgca aatgacgcga
     5701 ttgtggagtt tacaaagatt aaccctgaag atttaaacaa agacaattac aaagatcttc
     5761 taaaagatgt gcatggaatt ttagttccgg gtggatttgg agaaagaggt atcgaaggaa
     5821 aagttttagc aatcaattat gctagaacga aaaaaattcc attcttcgga atctgtctag
     5881 gaatgcagtg tgcagtcatt gaatttggta gaaatgtagt aggcttaaaa aatgcacatt
     5941 ctactgagtt taatccatct acagagcatc ctgttatttc tctcattgaa gagcaaatgg
     6001 agatagaaca aatgggtgga acgatgcgac tcggtgctta tccttgtctt gtcaaaaaag
     6061 gaaccttagc ttattctgaa tacaagtcag agagaatttc agaaagacat agacatcgct
     6121 ttgagtttac acttcgttat aaagagatgt ttacaaaggc aggaatggat ttttcaggct
     6181 attctcccga tgaaaaatta atggagattg tagaaatcaa agatcatcct tggttcattg
     6241 gggttcagta tcatcctgag tttcaatcca aaccaatcaa tccgcatccg ctttttgcag
     6301 gattcattcg agcagctgtt aaattattaa agtagaggta aatcatgagt aaacgcccga
     6361 ccgaaagaga atttctaaat ggaaatacga taggaggaaa taaacctttc ttcttgatcg
     6421 caggaccttg tgtaatggag tctagagatc ttctagatag agttgcaggg gagatgattg
     6481 agatttgtaa aaaacttgga attttttata ttttcaaaag tagttttgat aaagcaaatc
     6541 ggtcttctgt tcattcttac cgcggaccgg gcttacacga aggaattaag aatttagaat
     6601 atatcaaatc taaatacggc ataccagtgt taaccgatat tcacgaagta gagcaagtag
     6661 agcctttaaa agatgtgatc gatgtctatc aaattcctgc ttttctctca agacaaacag
     6721 acttactaga agctgctgca aagacaggga aatgggtcaa tgtgaagaaa ggacaatttc
     6781 tagctccagc tgattgcaag catatcaaag aaaaaattag agaatgtggc tctgaaaaat
     6841 atcttgtgac agagcgtggt gcaagttttg gttacaacaa tttagtcttt gacttgcggg
     6901 taataccgat tctccacagc ctagacattc ctattatctt tgatggaacg cattctgccc
     6961 aacttccggg tggcgctgga aatatcacag gtggtcaaag agaatttatt cctgatctca
     7021 tgacaggtgc agtctctctt ggagtcgaag gactttttat ggaagtccat cccgatcctc
     7081 cttctgctaa atccgattct actactcagt tctatttgaa caaagcagag aaactcatta
     7141 caaatttata taaacttgac cgacttgtta aaacagagct tgtggagtta gaaagttagg
     7201 cgggattgtt acaaattaga atcaaatact aaagggaaat catattataa attatagaat
     7261 gcaagaagat agtgcgttat ttgaaagttg ggaagagcca gaatatccga aatattctgg
     7321 gtattacagc ccaacctttg ctaacttaga tagtctttgg tatagaacta caacagattc
     7381 aaaatatgcc gatggaagag attatctaga ctctctttgg aatagagaat gcgataaaat
     7441 tatatttaaa tacatcaata aatacggacc ttatttttca gcctttgaaa tagatatatt
     7501 aaatgaaatt gaaaaagtaa tgaatcaaaa aattgatgaa aaaagaatta atttttttta
     7561 ctatatcgct accaaggctc atgaaataaa agaaattgaa aatatctgga tttctaaatt
     7621 tgaagatgaa tgtaaaaaga aagttgtatg caaactatgc aaatatgaag agtttaggat
     7681 tcatcaagag ccaaagattg taagagggag taacgcaaat tttatatact gctctaattg
     7741 ctacaaaaga actttaatgt ataaaagctt ttggaatgat gaaattgaac aaatttatat
     7801 tcaactagtc aatctagtaa ataataaagt tttagtgaat tgtaaccttt gcggtgtaaa
     7861 ttttgttcac tcgcccggca tttttcgaaa tgtcatgcat gactttgttt atccaaatct
     7921 atttacaact atttgtgggg aatgctttca aaaagcttat agtgataatt ggaaaagtaa
     7981 gaaagaaatt ttatatcata ctttgtataa attatttctt tatgtcggaa gaattcctac
     8041 ctacgaattt tatgattatc aatatctatt taaagaccaa gaatcaattt tacaattatt
     8101 tagttttttc atagatttga ggagtccgga aggatttaaa aaattagatg gaagtttcta
     8161 taaaacatta attaatgcag gtattttgaa atcaaattct cgaaataaaa ttttaggaaa
     8221 agtaatatta tcatcagatg gacatatatg cttttcaaat ctagaaaaag aaatagatga
     8281 ctttttattt gaaagaggac ttaaccacct aaaagatacc caatatcctg aaagtaattt
     8341 attaactaat tggaagattg aaatcgatga aaaaataatt tttatcgaat atatattaca
     8401 gacagataat gattatttta gtgatttaat ttataaaaaa aagtggatag ccaaagaata
     8461 taacatacat cttctttgca tttatccgaa agatgattgg aaaagaaaaa ttagtgaatg
     8521 tatcgatagt atataatgaa gactaataaa aaaaagcttc tacagctacg ctttcactaa
     8581 aacgaattcc tttttctttg gagttctagt tcgaaccgtt atttctaatt ctaaatttag
     8641 ttgtgagatg tagtcaatca gagtagagat tttaaaatca gttctagatt cgagtctaga
     8701 aatagcagac tcactaaatc catccatttc aatttgtttc acacctagtt cttttcttaa
     8761 aacataaata tttgcatata aaaatccata tattgaaacg ttaggaaaga tttattgtat
     8821 ccgtatgact tttcaaaaaa aactttttta tagacgcgta tgcatcaaaa aatatttttc
     8881 taaaaagact atacaaaagt ttgcttttct tttcatttgt aggaatgttc tcattacaaa
     8941 atagaaattt aaaaagtgaa gttggacaaa gtaagagtag cttgtcattt ggtataattt
     9001 tgacgttcct cataataata gttgcagcct tgtatattga cagtccatcc gatgctgaaa
     9061 agcaagaact ccaaaccata gaagagaaaa tgaagaatgg tgaacgactt aaagattggg
     9121 aaaaggatcg atttgttgag ttactttgga aggtaaaagg aattcggtta tttacttgca
     9181 aatccaatta tattactaaa ataggaagcc taactggata taaagtaact gaaaacgatt
     9241 tggattggcg agggactgga aaaactttgg atgatgctct taaagaagcc tttgctcaga
     9301 caggtattaa accagatgat tttcagcctt ctggtataga ggggttaacg gagacaggta
     9361 aaacagtgcc tgttgaatgg aaggtcgttg gaatagcaga agtaaatatc gatgctcctc
     9421 atacacgtag tggtccggat gtgtttcatg ttggatacaa agtttatggc agtaagaaaa
     9481 aagtaggaca tatcttcatg gactgtcttc cttattttcg tgaggttaaa taaatatggt
     9541 aaagaatttc ctttttaaaa tagcaaacga acataatttt aatttgaaga cgattccttt
     9601 ggagcaaagt aattttttac gcaatgatct ttatagaaaa tacaaagttg aaaatcgagg
     9661 gctattccta tgggatagtt atatccctaa ttcgtattta tcgaatcgag aagattcttg
     9721 gctcggatta gatgatttta tacaatctaa agaaacgata attttttttg atcaaaaagt
     9781 agaagcgaat ttctttgaac tattaagcgg acaatcgatt gtaagcctgc ttgaaaatta
     9841 caagcatgat gaatattatt taacgaaccg taatctagat tttctaattt gctgcactga
     9901 tctaggacat cttgtagccg caggcaccgc tcgcgattgg cttgaaaaac aggatttagc
     9961 ggtaagttct ggttagccgc aaataggaat tgcgtagatt ctttactgtc tcagtgattc
    10021 tacgacaatg attttaccca atcactgata tagagaaggc aagataagaa aaaactggtt
    10081 ataaaacttt caatgaaaac tgcgattgaa tattttgatt ttaattctgg aaaaatttcg
    10141 gattgtggag atccacttga gatagaactt tcttctcgcc atttaggttg gaaaggagtt
    10201 cttcttgaga aaggttggtc tccttatttt tatccaaata atatttatac aaactctttt
    10261 tattttgcac ttgcgttaga ctctcctctt cactgacatg ccaaaagcga agaggaatta
    10321 ctcctactca aaaccgatcc gggtgaagta tggatcaatc ctcccggaac accatttact
    10381 cacgaaataa aagaagcctg ttattttatt attttgctag tagaggaaaa agaaatgctt
    10441 caatgttttg atgagaagtt gcctactact aagttaaatt tcttaaacca atacaatgta
    10501 aaagacaata caattatgaa tattataaac cttttttggg aagaggtgaa gacgaacgga
    10561 agcaatggca aacaatatgt tgagatgcta aaaaaactct tagcaaatta ttttattaaa
    10621 aattattctg attataaaga tattctaaat caaaaagaat ctgtaaaaat taaagcagag
    10681 gacattgaaa ttgtaaagtc acatatttta tccaatcttg gttctgaaat atcaatcgat
    10741 actttggcat ttcatttgag tatgagtaaa ttttatttcc taaaagaatt taaaaaagcg
    10801 actggaatca ctccttatca atacatcttg aaaataaaat tggaagaagc caaacgattc
    10861 ttgcaagaaa cagaaaaaag tcttgtagaa atttctctag agcttgggtt ctcggatcaa
    10921 agtcatttca ctagaacctt tacaaaacag ttcggaatca caccaggtgt ttacaaaagc
    10981 aattatcttc aaataaatac caattaaata caatctatac ttttgaaacc attctaaaat
    11041 gaaactatct taggatttag gaaaggttta ttatgagtaa aaatgtgctg atcacaggag
    11101 tcggaggcgg ctttggaaag ttaatcgcga gagttttaac tgaggatgga tattctattt
    11161 ttggaactat gcgagatgtg aatggtcgaa acaaagagaa taaaattgaa ttagaaaaac
    11221 tgggcataaa agtatttgaa atggatgtga caaatacaaa aagcgtcgat aacgttatag
    11281 atcatattat gagcaaagtc aatctggatg tgttaattaa taatgcagga gtaggtgttt
    11341 tgggaaatca ggagactttt acaccagagg attttcaaaa attatttgat atcaatgttt
    11401 ttggtgtcca gagagtcaat cgtgctatga ttccttattt tagaaaaaat ggaaatgggc
    11461 tgatcattta tatttctagt ttgcttggta gaatttcagt gcctttttac ggaccttata
    11521 atgcaagcaa atgggcttta gaagcgttag ctgaaaatta tagaatagaa ttatccggat
    11581 ttggaattga atcctgcatt gtagaaccgg gtggatatcc aacaactttt attgataatt
    11641 taattcgtcc aagtgatgtg gatagaaaaa aacaattagg agagttcgct gatttcccag
    11701 aagagttttt aaaaggattt gaacatggac ttgcaagtaa tcctgaacaa gatcctagaa
    11761 acgttgcaat cgctattttg gagctaatca aaaaaccgaa aggcgagaaa ccatttagaa
    11821 cgatagtaga taagatggga atgggaactt atatagaaga atataatcag cacctttcaa
    11881 aagttacaga aggaatttac gcagcctttg ggatctctca tttacttact gtaaaaaaat
    11941 aaaatttata gattttaggt taattcgatt ttcatgttgt aaaacaaaca acatgaaaga
    12001 attctgataa gaattaatac taacgtttct ctgaattgat aatttagaaa gaagtctatt
    12061 ttattttgaa ttttacaggc aaattttaaa ggaaaagaat gataaaaaaa attactattt
    12121 tagtttttat tcttcattca atttttgcat atgctcagaa aacaaagata tccaaccctt
    12181 ttgatacttt tctatctggt gtttacagtc atgatactga taaactaatt tcttcctttt
    12241 ctgaggatgc aattgtaatt gatgtaggaa gaaagatagt tggaaaaaaa gaaattcaaa
    12301 aatgggcaaa gtcagagatc attgatgcag gactcgttta taaaatccta agccaaaatt
    12361 cgaaaaaaga aaatttgcaa gaatatatca ttcatatttc ttctaaaagt tttacattta
    12421 aagcaagata tctaatccaa accgaaaatg aaaaaatcat aaaagctgtt ttagaatatg
    12481 ctgactgaac agcagattaa acaagcaatt aaaactcttt ctgagaagca ccaagcggat
    12541 ggagctgaca cagcctatga actaataaca gaagcgcaga taaaataatt taagaaaagc
    12601 ctgatagtat agaactgatt acagtaacta acgttagtgg gatgaaagtt cggctgcgat
    12661 tctaccagaa acaagtccag caacagttcc aagaccaggc caagtattgt ctcctgtaat
    12721 acaaaaattt tcaatcccga tgtcttgtgg gactgatttc caattagaat tcgagagagt
    12781 ctgtttaaaa ccacctacag aagttcgctt tgtaaatttt tgataagtcc taggagttcc
    12841 tatttcatat acaactgggt ttgtggcaag actcggataa accctccttg cataatcgat
    12901 tagacgatca ccgatttctt ttttcttcga ttcgtattcc tcttttgaga gattttccca
    12961 ttctaagttt ttgcaatgag tagaaatcat aacacttctg aagccaattg gagcagataa
    13021 cgcatcttct tttcctgaga cagagataaa catattattt ccattcccaa gttctctatc
    13081 atagtcgagt aagatttgat ggtgggtaat tgattcagtt tcaatttctt tttctggaat
    13141 tccgagaaaa actacaatcg ctcccccttt ataaacttca tttttttgaa tatagggttt
    13201 taatttcttt tgaatgaatt ctggagagat tgtaaatgta ttatctaacg gaagagagga
    13261 aataatcttt tttgcttgta agttcccctt cttagtcgtt atttcccata ggttattttg
    13321 ttttacaaag gagatgactt ggtttgattt atagatttct cctccgagag aattgtagtg
    13381 attttctaga attttccaaa gtgctttcat tcctcctttt gcacgcatga gcccagcacc
    13441 acgaatcgtt gtgccaagag tcgcattgat aaaaggagcc tcttctattt tactatgcac
    13501 tgtatcttca atcagcattg aaagaagtcc aatgagaggt ttttcttttt ctaaatgaaa
    13561 aaattttaga acatctaaca ttgtatgatt ataatatttc aataatccca agttcttaaa
    13621 tcctatcgca gaaattgctt ttttaagttc agaaaatcta tgaattggga tttgtatatt
    13681 cttacgtgtg gcttcccaaa aaacgttagt gatccgatcg agtagttcat agaatgcaat
    13741 atggttcttt gaatttccaa gttttagaag tctttcctct ttccatctag tattgtctct
    13801 gtataaattt atttttcgat cgggaagcca tgctatataa tctaaatatt ctccttccgg
    13861 aattggtaga ccgatttctt gaaagaaatt tcctccaaca ccaccctcta taaaatcaac
    13921 cagagtagta gctcccacat caaaggaaaa atttttcttg gtaaaatagc cagcgcatcc
    13981 tccgatctgg gaatgagatt ctattactag agtattgaat cctttttttt gaagccttag
    14041 agccgcggta aatcctgata tacctccgcc aatgattgct acgtcataga gtttcataat
    14101 attttaaata aatacagaat tgaatgtttc gtcaatactt ccatggaaga agagattaaa
    14161 acccttatat aaaagattac caccgatagc atcgataact gaacgataaa aaacgatgga
    14221 ttcggttgaa taagataaca gaacaaaacc taactgttta gaaatacatc ggtgacaatc
    14281 gtctttccat ccaaaatcca ttgccgctcg ttcgtggcaa taggtgatag tcagttttcg
    14341 catacttgtt cgtaaggtta tgacacttga caggggagaa tacaaataat acttttattg
    14401 aatgcaaaag caaattttta caaaaatgag ttttggaaaa atcatttctt tctttcgaca
    14461 gatagtttct tttttgaaaa atcctgaaga tatgcataag ttgattttaa aattagcaga
    14521 agaatcagag gaggagtttg ttccaatttt actcaatcca tttgatccta tgctacttgt
    14581 aaaagatgca tcgattctac atttaatttt aaaagatgta gaatttcaaa atcggtcaag
    14641 ctttccaatt attgattata tttattttca gtttatcaaa aatctaaatt cattagaaaa
    14701 taagaatcca aagattcaca ccaacatggg gggaataacg tttacaaatg gagaggaatg
    14761 gaaacgaaat aggaagatag gacttactgt catgaatgat ccaatctttt tggagaagag
    14821 tattgaacca attctaaatt ctattgatgc aatgctttta aatttaaaag aaaagaaaac
    14881 tcagtttgca atcagtgatg aattgagttt acttggaatt gatgtagtag ggaaattagt
    14941 ttttaatatc gacttagaat tattttcgaa agagaaaaga aacaagaatc aaatcgattc
    15001 ttattttcta acaagttctg ttcgagtgat gcttgatgca attcagaaat acatttatta
    15061 tccatttcct gctttcctat tttctttttt caaaacgaaa gacattcgga atttagatag
    15121 agcagtttcg aatctggcat tggctggaaa tactcttctt tcaaaagtaa aagaagattt
    15181 acaattagga aattctagaa gcgaatcttc gattgctgga attttattac agaaatatcc
    15241 agacttagaa gatacggaaa ttcaaactct tctcatggat ttacttggag ctggacatga
    15301 tacaacagcg aatcttgtga tttttactct ttctagtatt ttagaatcaa atgttttaga
    15361 aacttcaaag gaattgcaga aagaaatagc gtctatcgtt gagattttac cttccttcca
    15421 gaaagcgaat gagaatatag attctaaatt gattttagaa aaaatagaaa agaatactcc
    15481 gatcttaagt tcaattctaa atgaatcctt acgacttttt ccacttggtg ctgtttttag
    15541 tcggattaca ttttctaaaa aagaaattca ttttaatatt aaagataagg aatatatttt
    15601 aaaaccaaaa tcgaaattac ttgtatctcc ctatgtaaca ggtcgtttga aaaaggattg
    15661 ggggaggagt gcaaatgaat ttgatccatt tcgtttttta gacaaagaaa aaggaaatgc
    15721 ttttattcca tttggatctg ggaagagaag ttgcattgga gctaggtttg gaattttaga
    15781 agcaaagctg attttatttc gactctttca aatttacaaa atagaaaaag gagaatcctt
    15841 tcaaaaaaca gattctattc ttgcttttac tcttagagca aaaaatccaa tctgggtttt
    15901 agcgaaagct aacacttaaa aaagaatata aaagattacc accgataatt aaacgataaa
    15961 aaacgatgga tctttgtgag cttattgtat ctgtatcgat actctttggt tgtatagaga
    16021 ggatcttatt tctttggttt tagccgaaaa ctacgcttcg catactcatt gagcccatat
    16081 ctatagagtc attaaaaaaa gaataactgt cgtttgcctc tctgagtcca agaatataaa
    16141 ggagaggaag ataatggtcg ttagtcggat gagcaagact tgcaatctct cctagatttt
    16201 tatattccaa aagagcttta tcatttttct cttctatatt attttttaca aactgatcaa
    16261 actcaagtgc ccagtcgaaa ggtttcgcat tttcttgcca tctcacctgt cgtaaattat
    16321 gaaccacatt tccactccct aaaattagaa ctcctttaga tcgaaggctt ctaagctctt
    16381 ctcctaattt gaaatgaaat tcgagggact tgtaataatc aatgctcatt tgaaaaacgg
    16441 ggatgtctgc ttttggatac atatttctca aaacagccca tgttccatga tcgagccccc
    16501 atttataatc ttcctgcact tctgctgatt tgatagcgtc agccgccata cgtgcatagg
    16561 ttggggaacc aggagctgga tactgaacct gaaaaagttc atctggaaaa ccaccaaaat
    16621 cgtgaatggt ttctgggttt gtagccattg taactcctgt tccgcgagtt agccaatgag
    16681 cggaaataca aagaattgca gttggctttg gaatggtatt tgcgaaagta gcccacagtg
    16741 ttttgtacgg attttctgta attgcaaaca ttggatttcc atgtccaata aatgcgactg
    16801 gcattttctt ttctgtttta ctttctaata aatttccgaa ctcttttaaa ttcatatcgc
    16861 aacctgctat tgtgaagatt gacacatatt tcaaaaactc ttttctattc atttttaata
    16921 cttatctttc tagagtgaat caaaacatac acatcttatt tttacaggat tgcgtgcttc
    16981 taaatgcagt caaaacttaa tttgagtgtt tttatagttc taaaattctc tatcaatctc
    17041 aaggttcttt attatagttt acaaaataga aagaatggtt atacaggaat agtaagacat
    17101 gattcgttta ttagatactc ttaaactaaa atatacaaag agacttttcc tttcctcagg
    17161 agcgatttct gtgcgtttgc ctcttagctt aaatattgcg ggcaagattt atcatttaga
    17221 gacaaatatg atttcgagag agtatatttt tattaaaatc tatgaagaag attttgagtt
    17281 tttgccttcg gtttattctc atataattct atttatgaat ttagatctgt ttaatattgg
    17341 taaatatgaa ttagaaggaa agcttgtgtc gattgataag attgcgggga gtaggtgtgg
    17401 tctttggatc gagtataaaa cagagacaga atcagatcgc aaaacaattt ctcaatttgt
    17461 tcaaaaccat tatactcccc gttacagtgt gcgattctct gtggaaatta aaacagacca
    17521 taaaacgtta cgcggagagg caattaattt atcagaaaaa ggaatcttta ttgaagctga
    17581 gttagggcat ttggaagaaa ataaaatgta tagtttgatt ttatatttag aatcagatat
    17641 aattcatgtg aatgcacaag tgagctggat caataaaggg aaaatgtacg acaaaccaaa
    17701 tggatatgga cttaaatttc taccagataa aaacgctgaa aaaaaaataa cgcaatattt
    17761 aaatactctt aaaaaaagat ctgctatact acgctaaatt atttcttaat ataaatatat
    17821 cctcttccgt ttttgatttc caattttgtg tttaaatttt gacaaagtat agaatattca
    17881 tgtaaaaaat cttctgcttc cgagtaggga agagggcgaa cgatatgttc tgcatcctga
    17941 aattttccaa agataggaat tacttcacaa aatattaatt cattattttc aaaatgtaac
    18001 ataacggcta tattgtgatt taggtattga tttctacttc cgaataaaaa gttacccaag
    18061 gagtaaataa taattccgtt tttatatttt tcaattcctt gtgggatatg aggatgatgg
    18121 cctatgatcg ctgtgaatcc agaattaatt aattcatgac cctgtttgat ttgctcttta
    18181 gttggttcag gactgtattc tactccccaa tgaacagata gaagttgtat tttgccttgg
    18241 ttgtttttat cagaggatac agtttttaat cggtttattt gtagtggggc tacgcctggt
    18301 ttaccttcac ctgcaaacaa cctagattct ccaatattgc tgactgaaat gatttgaacc
    18361 gatttgtttc gaatcgagat ttgatttggt tttatagctt cccgaatgtt ttttccaatg
    18421 cctgcaaaca agatcttttc tttgttcaga ttttcaatgg tttcttcgag accttttttc
    18481 ccataatcca tggagtgatt gttgcccaga aacacaagat caatcttaat cttttttaag
    18541 agacttatat cttctggttt tgcattgaaa acatatgact tagactcgtc catatcatct
    18601 gttgaatttg caacaggagt ttctagattt accattctta tatctgcttc taaaaaaaga
    18661 ggaatcaatt cttcaactgg cgcgaattca cctctttttt gcctagtttc tctaagcccc
    18721 cagttaaaca ttacatctcc ggcaaccaag acttttagtg ttctggtttt tttctgaaca
    18781 tccgaaaata taggagtttg cagaatattt aggagcaata gaatcaatac caaataggtt
    18841 ttcttaaaaa gtctcgattt gaactgcttg gttttgtaac aagatctggc ttttgggttt
    18901 tggttggaaa gagtcatttt ataaatcgag aatgtatttt tttttatcat atgaaccttt
    18961 ggaatactgt aaaaaagtaa ttgtctgtat actcctagta aagcaaatag ttagtaaaat
    19021 ataaaaaagg ataagaatta tgaaaaaaat cattttctct ctggcaacat tactttcaat
    19081 ttccaattgc attgtattag ataaacttgg tctcaattta gtcaaggaaa cagtggaagg
    19141 acaagccgca attaatcaga tattaactgg tgcattgatt ggtgcagctg ccagcggatc
    19201 tacaacgact tttgcaactt caatcgcaac atataataca aaaggactga aacatagtaa
    19261 gttctatgac aaagatgatg tagatgcttg tgcagataaa gttattattg ttgcaaatgc
    19321 tgtgagtaat aatcaataca caagaggatt agccgtgggt gatattgctt gttctccgat
    19381 tcgtgagcat aaaaccttga tcgattggcc agttccttta ttctaattac atacctatga
    19441 agaagagcat atcatctaac gtagataggc tctgttttct attggctttg aggctttata
    19501 ataagcctag tcttacagtg ctgtaaaaaa gtaattgtct gtatactcct agtaaagcaa
    19561 atagttagta aaatatataa aaggataaga attatgaaaa aaataatttt ctctctcgca
    19621 gtattagttt ttgtaacaaa ttgcgtatta attgatagct taggtttaaa cgtaacacac
    19681 gctactatca aaggttctga ggcaaaaaat cttatattaa ccaatgcttt agcaggtgca
    19741 gctctttctg gagcagcagg aacttctgtt gcgactgcga ttgtaactta caatactaag
    19801 ggcataaaag atagtaaatt ttatgataaa gatgatgtag atgcttgtgc aaataatgta
    19861 ttagctgtta attatgctgc tgttcgaagc acaggagtcg ctcctggtga tttaggttgt
    19921 ggaagcattc gcgaacacaa aatgatcatt gattggcctg ttcctctttt ataatccaca
    19981 ctctagaaac aaaaagagca gattcttatt gctaagaaat ctgctctaat acgggcttaa
    20041 ataattttta tattatctaa gaagttgtag cacagactga ggtcttacgt tagcttgagc
    20101 catcatagca gtaccagact gaactaggat ttgattctta gtaaacgcta cagactcttc
    20161 agccatgtct gcgtctctaa tccttgattc agaagcttgg atattttcat atgctaccat
    20221 gagtccttta gacgcatgtt ccaatctgtt ttggtatgca cctaaatttg ctctttgctt
    20281 gtttactttg taaagtgcat cgtcaatagt tccgattgca tcatttgcta agtcagctgt
    20341 agagagagat aacattgttc catctggtct ctttaattca agagctctaa cagtcatagt
    20401 agcaatgtaa actctttctc tttgatgcat gtttgcacct acgtgatacc acatagaagc
    20461 aactctagaa ccacgagcaa agtcaccttg taaaaggttc attttgttga actctgcctg
    20521 agaagctatt ctatcgattt catcgattaa ttgagaaact tcaacttgta tcatttgtct
    20581 gtcttgagga gtgtagatac cgttagaagc ttgaatagct aaaacacgaa ttctttgtag
    20641 aatgtcgttt gtttcagtca gataaccttc agttgtttga atcatagaga ttccatcttc
    20701 agtgttacgt tctgcttgtc tgaggccaga aacttggctt ctcatttttt cagaaactgc
    20761 aagaccagaa gcatcatcag aagctctgtt gattctcatg ccggaagaaa gtacttccat
    20821 attttttgaa acttcttcgt tttgaaattt caaaaccctg tgcgagttaa tcgctgataa
    20881 attgtggttg atgatcataa gtttcctcct tgaaactgat cacaagcaac tgggagcatc
    20941 cttgcccaca gtattttgtt tttctttgtg tgaatttttg tgtctgattt tatttatttt
    21001 tgtaagatat gtatcggaaa ccaaggattc agtattaatt tctgaaaatg ggggttaaat
    21061 tggggtttta tgggtttttt tagcgctaaa catgtaaaat tagcgttttt tgagttttta
    21121 ctttttttgc aaaatttaaa aattttttcc gctaacaata atttcggaag aatttcgaaa
    21181 aatgcattag cacttttttt atgatttttt tttaatttgt tccaaaaatg agaaatttct
    21241 ttagactcat aggattcgat attctttatt ttggtgtggt tgcttgtaaa atcttactcc
    21301 acttctgaat tgctttttca atcgttcctt ttacaacggg cttagtaata taatcattca
    21361 tacctgcttg gatgcatttt tcaaaatcac ccttttgggc tcctgccgta agtgcaatga
    21421 tcgtacacgg tctttcttgt tccaattttc tgatttctct agtcgcttct aaaccactca
    21481 tttccggcat ttgtatatcc ataaagatta aggtaggatt ttctgtttta aagatttcaa
    21541 tcgcatcttt tccattctct gcttgcatga taatcgattg aggaagaata ctttttaaaa
    21601 tcgtcttggc taaaagtaga ttaatggaat catcatctac aactaaaacc ttggagaaac
    21661 ttagctcttg gttagatgat tcagtttcta ttttattagt ctcttttact tttttgtctt
    21721 tggttgttaa gttgatgatt tcactgaata aatcatatgg ctttagaggt ttcattcttt
    21781 tagatagaat atgtttgtct tccggcattg aggcaaaatc gccttcttca agagcatcat
    21841 agagtagaat acttggaatt ggttctatat atttttttat aaactcaatg cttgattcac
    21901 ctggtaaatt tgtatcaacg atagcaagat cgtatttttt tctagatagt aaaatcttcg
    21961 catcctcaag gttaaatgtt acttctgatt caatttggaa gtagtttaaa aatttttgaa
    22021 ttacacctgc atttgatcga ttcgtttcta aaatgattgc ctgcttaaca ttcgtctcca
    22081 ttggctcgat gagtttgtct ttttgaaatc tacattcaat tgaaaaataa aatcggcttc
    22141 cttttcctaa ttcactttcc agttctaatt gactattcat tgtattgagt attctgttag
    22201 agattgttaa acctaatcca gtacccccat attttcttgt ggtcgaacta tcttcttgtg
    22261 taaatgcttg gaaaatacgt tttgaatttt taggatcgat tccaattcct gtatcgcgaa
    22321 cagagaaatg taaaataatt atatcctttc ttgtttctgg aaaattaaaa tctatcttga
    22381 gttcaatttc acctttatcg ataaacttga tcgcatttga taaaaggtta atcaatattt
    22441 gccttaaacg gaccatgtcc gtccaaatat actttggaat atcaggattt atattataaa
    22501 tcaattcaat attcttttgg tgggctttga atttaattac atctattgct attgcggtta
    22561 actcgtatat atcaactggt tcaatgttta aatcaatttt ccctgcttca atttttgaaa
    22621 agtccaatac atcgttaatt agatctaaaa gcgaattgga agattgtgaa ataatcttca
    22681 tatattgtct ctgattggag ttcatttccg tttttgagag tagatcaata aaaccaatca
    22741 ctccattgag tggtgttcta atctcatgac tcatgtttgc aagaaattcc gacttggcac
    22801 ggtttgcgag ttctgcttgt tgaatttttt cttgtaattc tttgtttaat ttttttcttt
    22861 ctgttatatc taaaaagtta accactgaac caataatttt tccatttatt agaattggat
    22921 acgaccaata ttctacagga aagaaagtgt tatctgcctt tttaaaaagt tcggaatcgg
    22981 aatgaacttt ctccccttta ttatgtgcag ttaaaatttt acaattttca tattgaattg
    23041 gctttccgga ggagtccgag tgctttaaaa aatggtgcat attcttacca agtaaatcat
    23101 caatacttgt gtaacctaaa atttctacac aggcaaaatt gcaaaaacta caattgccat
    23161 ttagatctaa gccgaaaatt gggtcggcag tggagtttag tatcatccta aaccactctc
    23221 tgctttcctt taaggcaagt gtcatactat cagcaagtgc aattgccttt ttttcagtat
    23281 ttatactgat tttggtaaga aagaataaaa gaaagctcac aatgacacca gaaatgaaaa
    23341 cgatatagga acgattaaaa tagaaaaagt tagccttagg tcttagatca gtgaatctta
    23401 aactccatgt ggaaccaaaa atgcttatag gaattacttt ttgagatatc tttagattaa
    23461 aattctgctg aggaatattt ccactatcgt aaagtaggtt tccttgttta atttcttctg
    23521 attcaaatat ttctaaacgt aaattttcac ctgaattctg gttgctaaga attccttcta
    23581 aaagatcttt catacgatag ggactgtaga cccaaccttg aattgctttt cttctttctt
    23641 cgattgtagc aatcggcata ttctttttgt agaatggata cataagaaga gtgccaggct
    23701 gaacatcttc gtttgtttcc tgaacgagta taacttttcc ggtaagtcca ggagtatgat
    23761 agtctctcga aaattctaag gctttacgtc ttatattctc tgaatacata tcataaccaa
    23821 aagctcttaa atttctaccc acaaaaggct ctaaataaat aatagaggaa tagatttccc
    23881 tctccccctc ggggaatact ttataatttg gaaatccttc tctccgaacg ctttctatat
    23941 gttcttggag tttagttttg ggaattagga tcgttacacc gactccaagg attccaggga
    24001 ggctttggat tgcttctgat tcgtctatat aggttttcca gtcttttcgg gagatggaat
    24061 ctctactttg taaataagcg gctccgtctc ttaaaatttg tgcctgtgat tctagtcttg
    24121 taatgatttt agatttgatt tcattgcaca gaaaactaaa ttctaaggcg ttggtggaaa
    24181 tactgctttc tcttagaaaa taacttacaa tgcaagttag actaaaaaaa atcgttaata
    24241 atatccatgg aatctggtca gagcgatgta agttttgctt catttggttt tattataact
    24301 aaaaacaata tagatactga ttttatattc aattagaatc tgaaatgagc ttaatttgaa
    24361 aacatttttt tagatgaaag aaatagaacc cttctcaaaa attgagaagg gtaggagtta
    24421 aattttgaaa ttatatttct gataatctca actgtttgct tctgaaaata attttactct
    24481 actttttcct acaaaccagt aatatagaac agggacagca aaacgactga gtgctgtagc
    24541 tgcaacttca ccgaacatta ggcttatcgc cagaccttca aagataggat caaagagcat
    24601 gataaagctt cccacaacaa ctgcagcagc cgttagtagc attggtctaa atcgtatgac
    24661 acctgcacta attaccgctt cttttagtgg ttgtccctct tttaattgtt cttctataaa
    24721 gtctactagt atgatcgagt tacgaacaat gatccctgca cccgcaataa aaccgatcat
    24781 tgaggttgca gtaaaatatg ctccgagcat tgcatggcca ggaaggattc cgattaaact
    24841 aattggaatc ggtgtcatta taataagagg cacagtgtag cttttaaacc atcctagaac
    24901 taatatgtag attaggatca taacgactcc aaaggctcct cctaaatctc taaagacttc
    24961 ataggttata aaccattctc catcccactt gattacgggt gattttgtat tccaaggaac
    25021 atctgctgtt tgtgtaggat aattgatgtt aggcgcaagt ttcaacatac cgtaaacagg
    25081 agcttcttct gctccagaga gttcacttaa aacataggag acaggcttta agttttttct
    25141 atgcaatact tcatttgaaa ttagtttagg attttttaga agagaatcaa caggaatcac
    25201 accagattca aaagaagcaa ttttacttcc ttggaatgga atctgacttg agcgaacttt
    25261 attgtcaacg gatagaacaa cgttagtttc ctcgggtgca tctgcttctg caagagaggc
    25321 aagattggat tcggtaaaaa tataggatcc aactccaaca acctgcattg attttgctcc
    25381 caatattcca cctttgattt gatcatagtc ataaactttt cttgttttac tttctctcca
    25441 agaataatct aagtctacaa ctgatttttc tttttcaaag atggagtaaa tttcttttgc
    25501 tacctgacgt ctgatttctt cagtcggacc ataaacctca gcaaccattg tagccatgac
    25561 tggaggacca ggtggaattt ctaaaacttt tgtgatcgca ttcttttcct ttccaaattc
    25621 ttggataata ggtcggagtc tctcaatgat tttatgacta gattcttttc tttttccttt
    25681 atctgagaga agaatttgta gatcattcat ataatcttct tttcgtaaga atgtatgctt
    25741 taccattcca gagaaggaaa atggcgcact ttctccagca tagatttgaa ttttcttaac
    25801 ttctttttct tggagtaatt ttgcagataa ttccttcgac cactctacac ttttttccaa
    25861 aggtgtatct ggtggataat caatggtaat ctgaaattca tttttgttat caaatggaag
    25921 catcttcacc ttaacccatt taaagtagat aaaactaaaa gatgagagta ggaggataat
    25981 gctgattatc ccaaataaga aggctgcttt ttttgttcct agtaacattt ctgagaaaga
    26041 aatgtatagt ttatctaaaa agctttcttt aggtgtttct tcactatggt gagtaacggt
    26101 ttgctttaaa agtcgaacgg atgcccaagg agtgacaata aaagctacaa ataaggataa
    26161 aatcatagct aaacttgctc caacaggaat cggtttcata taaggaccca tgagtccttg
    26221 cacaaaagcc attggtaaaa tggcagcaat tacagtaaat gttgctagaa ttgttggatt
    26281 cccaacttca gaaactgcat tgatagttgc ttttagaatt ccagattcag gatcgagatg
    26341 taaatgcctt tctacgtttt ctactacaac tatcgcatca tctactaaaa tcccaatcga
    26401 gaaaattagc gcgaataaag tcacacgatt taaggtataa cccatgaaat agtaaatcgc
    26461 gagtgttaag gcaagagtaa cgggtattgc gatagataca acgattgcag atcttagtcc
    26521 cataacaagg gcaattagca cagtcactga aatcgttgca atcagtaaat gttcaattaa
    26581 ctcaaaagat ttttcttctg ctgttgttcc ataatttcta acaatagaca ttttaatatc
    26641 agctggtaaa tcttttgtaa aagtttctgc tctttccatt aagtcttttg ctaaaactac
    26701 aacgttagtt ccttttcgtt tagcaaatac aatggagact gcatttttag gttcagttcc
    26761 attatcttta tcatacaata ttgataatct atttctttct tctgcggcta attggatttt
    26821 tgctatatcc ttaaggcgaa caatgcttcc tcctctttga ccgattgcaa gatttccaac
    26881 ttcttcactc gaagataatc tccctcctac ttcaacttga aattcttttt ctttagaata
    26941 tgtttttccc attggaaaat aagcatcact cattttaaga gtatttgcca catctaaaac
    27001 agaaacccct tggtttgatg ctttgactgg atctaaaatg attctgatac tttctcttct
    27061 tcctcctagt aattctacac gggaaagatc tggagtgcta gaaagctcac gagcgagtgg
    27121 tgcaattttt tttcttagat catagtcatc tctttccgat gagctgaaac ttaaagtaag
    27181 aaaaggaaca tcatcgattg taaaagaact tatcttaggt ggaagaacgt tagaaggaag
    27241 atcttgtcta atttccatca atttatgatg aacttttaca agggatggtt ctgtaggttc
    27301 tcctacttta aaacgaactg tgatgatact tccatgggct ttcgttgcag aataaacata
    27361 ttctactcca tctaatcccc aaacagccct ttctactttt tctgttacct ttctttctac
    27421 ttccttcgct tcaaatccag gtgcaaagat ttgaatatca atcataggaa ctgaaatttg
    27481 tggctcctct tctttgggag taaggtaaac ggacattagc cccaaaagaa tacttacgat
    27541 tgcgataact ggagtaagcc ttgaatttat aaactgctca gcaagttttc ccgcaaaacc
    27601 tttttctaat ttactcatat tttaatcctt ctatatttgt ttcagataaa gttaaaattc
    27661 cagctctagt tttaagatat tcagattcca aagttgattt ggctaagata atatcagctc
    27721 gcctagaaag aacttcagct aattgaattg cattaattgc tccacttgaa aagagctgtt
    27781 gggatacagt gatttgctct tccattaatt ttgtattttc ttgaatcaat tccaagtttt
    27841 tattaagagc tttttctatt tgaactaatg atttagattt tgcttcttcg tttcgaatgg
    27901 tttcttctgc tctttcttgc attgctttac tatttaattt tgcttgttcg attgagtcca
    27961 agtctcccgg agagtataaa ttcatttgaa tataaaaacc tgcattaaat gaattggcag
    28021 ttgctctatc tcctctatac agataagatt ctccatagag acctactttt ggtagaaccc
    28081 ttgctttctc agcttcagct tgttctgttg cactttctgc atacgatttc attgcttttg
    28141 ccatgtaaga tttatctggt gcttgtagtt ttaaatattc attcgcaaat gacatagcag
    28201 attgagactt taatgtccat ttttcgggta acgtttgggc agccgtttca atcgaacttt
    28261 ttattgaatc gattcttgct gtagtctctt ccttaattcc atcaattcga ttttttaaac
    28321 tcttaagtgc aattccacct gaatatccga ctggattgcc tctatttcct aattgataac
    28381 gatttaaaat ggaagttaag actttttcta atgaatttat tttctcttgc tgctcgttta
    28441 aagcaatgag tgttccatac atagcagcaa aatttgcata ttcatttaga gtaacaaatt
    28501 tattttctaa ttgcttaccc tctgattgct tttcatatgc tttcgcaacc gcttttttgg
    28561 atccaccttc gtaaattgaa aaatcgatac cgagagttcc tctttggtaa gtatttgtgc
    28621 ctgggtagtt taatgtatca ggtgcaaata aattaagtgt attgtaattg ggagttgtat
    28681 agagttgatt gtttgtatct ataaaattgc tcggttgtgt tcgaacactt ttagtagaga
    28741 aatcagatgc ggctgcagat ctctgaccta gtgttgagaa aaagtttaat gccgggtcat
    28801 ttgttgtaaa gttcctagca tctgcataca ctcttggaag ccaatgcttt gaggctttgt
    28861 tgctagaaat tttagccgcc ctcgattcta gtgataatgc tttttgtgaa tgggagtttt
    28921 gttttatttt ttcccagata gagttgaatt ctataacatt ggaatcttct gccaatagcc
    28981 caaaatacga gctaaagaaa agtccaatta aaattaactt tgttgcattt gatttcattt
    29041 taatctccta aatataccat ggggggtata gtatctataa attcaatctt tttttgcttg
    29101 tcaaataaaa tattataccc cgtatggtat atggaggcta tgaaatgtat ttagcaaaaa
    29161 cagataaatg gtatttagaa agggtgattt ggcttcttgc tggtttgttt acactggcta
    29221 gtttagggtt aggttatttt gtaagccctt attggtttat attgacatta cttgtaggaa
    29281 tcaaccaaat cgttttatct ctcactggtt tttgtcctat gactgtaatc ttggacaaag
    29341 cagggtgtca ttctaggatc agttaaattt tccaaaaata atgcgggtta gattttatat
    29401 ctaactcgca atttttcctc aaaaactcta ttctgctttt cgggtaagta tcgcatctaa
    29461 ttaaaaatct atatttagtc taagttactt tagatacttc tattgttatt aaattttatt
    29521 tcaaatgctt tttgattgcg tggattgaat taaaatattt atatatcatg aataattcga
    29581 tggtagtttt tataagaaat attttatatt tttcatctat ctttggtttt cagatagtat
    29641 tctgggaatt tttgaaacga tttgattatt ctattgcgat cctaattctc ttttttgcat
    29701 ttgatctctt tattattatt ttcactataa attattttaa aatatttcta atttcgaatg
    29761 acgctcctct actaccaaat ctactattac gtgatgaaat tatttgggaa ataaactcaa
    29821 aaggagatca atttctaaat atctgtgcga gggatgaaag acttttaaat tatacaaagg
    29881 aagaatggct aaaaccaaat tttttagaag attttatcta tccactcgat aaagagatta
    29941 cctttaaaaa ctttcaagat atgattcaaa tcaaatctga gtgtaatttc tcgtttcggt
    30001 tttatactaa gaatagaaac attgtttggt ttgaagcaaa attagaacaa ataaaaaatc
    30061 aaaatgggat tgaacatttc tatggaacgt taacagatat aacagagcaa aaaaagaagg
    30121 aatctttact tcaatttcac agtgatattt taaataaaat gtctgaaggt gtttttttgc
    30181 ttagaacaag cgatgccaaa attatttata caaatcctag ttttgatacc ttatttggtt
    30241 ataagaatgg tgaattaatt ggaaagcatg tgagtatttt gaactcatcg gatactaaac
    30301 caagtagaga agtagctgat gaaatcaatg caaatttata taaagatggt ttttggagag
    30361 gggaagttca taatcaaaag aaaaatggcg aatctttttg gtctgaagta aatgtatctg
    30421 aatatattca tcccgaatat ggactggttt ggatatcggt tcacaatgat attacacaaa
    30481 gaaagaaaat ggaagaggat ttagtaaaag caaagcaaga tgctgataag gcgaatcagg
    30541 ctaaaactga ttttttagtt cgaatgagtc atgaaattag aacaccaatg aatggaatct
    30601 taggaataac gtctctcctt gttgaatctg atttatctcc tgaaaaaaag ggctacgcag
    30661 aacttgcagt aagaggagtt gaagtattag aaagccttgt tctgaatata atggattttt
    30721 ctaaaataga atcaggaaca attcatattg aaaaaactga atgtaattta tcggaaatga
    30781 ttcattcaat tttttccctc tttgaattac aagcaaagga aaaaggttta gattttaatt
    30841 tagaaatgga tataaaattc cctcagattg tcatttgtga tatgcataag attcgacaaa
    30901 ttttgattaa tttaatttca aatgcgatta aatttacaaa taagggatat gtaaaaatta
    30961 aacttcgagt catagaagat ttgggagata aaaaaaattt agaaatttta gtccaagatt
    31021 caggaattgg aatcaaagaa gaacaaaaga ttcatgtatt tgaaagcttt tttcaagcag
    31081 actcttctat gactcgaaaa tatggtggtt ctggtttagg tctttccata tctaaaaaat
    31141 tagtcaatat tttaggcggc aatctttctt ttgaatcgaa ggaggggatt ggctcaactt
    31201 tttgtctatc catgattgtt gaaattccaa aggaaagagt aaaaaattct acaattgaaa
    31261 aagtatccct tcataaagaa aatccgaaaa tattgatcgc agatgatgat gatttaaatg
    31321 cgatggttgc gattcagata ctaaaaagca aaaatattca taatgttacc catgtctata
    31381 gcggtttgga tgcattgaaa gaaattgaaa catcggatta tgatttaata ttcatggact
    31441 tacgaatgcc ttctatggat ggaattgaaa ccacagtttt aatccgtaga ataagtgata
    31501 ttccaattgt tgcgatcact ggtgaagtga ttgaaggcga gcgagaaaaa tgtttagcgg
    31561 caggtatgaa tgacttctat acaaagccaa tgtcggcctc tgattttatg aaaattattc
    31621 atgagtggac ttaataaatg gaagagaaat tcaggaaacc atccaatcct aaagaaaaag
    31681 taaattgttt tccccagtta tacctaaaag ttgaatttaa attaaattta aaacggatca
    31741 acaatacccc aatttaggtt ttctatctaa ttgtaaaatt tactcttgaa ggaattgatt
    31801 ccctgtggga acttgtaaag tattgaaatc atttaggaga gaatttgaat gagtaaaatt
    31861 aaggttaaaa caccattagt cgaaatggat ggggatgaaa tgacccgtgt gatttggaaa
    31921 gaaatcaaag atagatttat acatccattt ttggatattg aattagaata ctatgatctt
    31981 tctgtaacat acagagataa gaccgacgac aaggtgacta cagattctgc acaggctaca
    32041 ttaaagcatg gtgttgctgt aaagtgtgcg actattacac ctaacgcaga tagagtaaaa
    32101 gaatacagcc ttaaaaaaga atggccttct ccaaatggaa caattcgatc catattagat
    32161 ggaacagtat ttagaaagcc aattattata aataacattc cagctgctgt tcgttcttgg
    32221 aaaaaaccaa ttagcattgg acgtcatgct tatggagata tctacagaga tgtggaattg
    32281 caaattccta gtgctggcaa agtagagcta gtttattctg atacaagcgg tgcagaaaag
    32341 caaagagttc ttgttcatga atacaaaggt cctggtgttg taatgggtat gcataattta
    32401 gataaatcaa ttatcagttt tgcaaaatct tgctttaact atgcgttatc tgaaaagatt
    32461 gatatctggt ttgctacaaa agatacgatt tctaaaaaat accatgctag atttcgtgct
    32521 atatttgatg aaatggcagc cgaaaaagaa gcagaattga aagcggcagg tatatcttat
    32581 agctatttct taattgatga tgcagttgct cagattatga aacaccaagg cggaatgctc
    32641 tgggctctta tgaactatga tggagacgtt atgagcgata tggttgcttc tggttttggt
    32701 tcgcttggtc ttatgacttc tgttctcgtt tctccagaag gaaaatatga atatgaggct
    32761 gcacatggaa cagttacaag acactatcgt aaatatcaaa aaggggaaac aacttctact
    32821 aactctgtag cgtctatctt tgcttggact ggtgctcttg cgaaaagagg ggaattagat
    32881 ggaactcctg aagtaactgc atttgctcaa cgattagaaa aagcagtgat tgatacaatt
    32941 gaagcagggg agatgactaa agatttaatt tctttaagta ctgcacctgt aaaaaaagaa
    33001 ttagatactt tccaatttat ggaagcagta gctaaaagat taaagtaaaa aagtttagag
    33061 agactgtgta atagcttaaa aaatctaacc acggagatcg cgaaggttaa aatttactag
    33121 gaaatttaca atttttcttc gtggccttcg tgcaattcgt ggtgaaaatc ttgcttttgc
    33181 acagtcgctt caattcttaa atatccaaaa agtcttttga cttcaaataa ctaaaactat
    33241 cctaaccaaa cttttttatt ttccgcagca tactcatcaa aagaaatagg atcttttcct
    33301 gtgatttttt taatcgaatc aatgattgga gaagaatggc cttgttttaa atttcccgca
    33361 ataaacacca tgaaatcggc atagtcttct tttaagccag cacctaaaag tgctttcttg
    33421 aaatcttccg gggtaatgtc tacataggat atagatagac cggtagcctt tgataattta
    33481 gatgcaacgt ccgagtgtgt gatcgcctct tttccagtta aagcaaacgc ttggttttta
    33541 aattcatctg taagtaagag agtaattgcg gtagacgcaa tatcttttgc atgaataaaa
    33601 cttactttcg catctcctcc ggggaagtaa atttttttat cacttaatat tccactaatc
    33661 cagtaggttt ggaagttttg cataaaccaa tttgggcgaa taatgttata acttaagcct
    33721 gattgctcta acgcaatttc taattttcta aatggtgctt caggaggagc atgctctaca
    33781 cccattgcag tcattagaac aaattttttt attttagatg attttgcttt ttctagccaa
    33841 ggatttaaaa tatcatattg atttgtaaga ccaggcggag agatgaaaaa agctgcatct
    33901 actttttcta aaacttcaac accttccttc ggtttttctg catcagcaat tacccaattt
    33961 agattggttg ccttttttcc tttttctgga tgtctagttc ctgcaaagac ttcatggcct
    34021 ctttctaata gtccatttac tacattactt ccaatacttc ctgtcgctcc gtatacaaat
    34081 actttcattt tgtctcctaa tttatataaa aatattttct tattagaaaa aattcttcct
    34141 ttctctaaaa aaatattcat ttgaaattct taagtataga attaatctaa actatatttt
    34201 gaatatctaa ataatatact tattatatct aaaaataaaa aattcatatc cattcgtata
    34261 aaatattaga cttatagata taattaaata ttctaagaat atggatatac tttctgagat
    34321 aattaataat gcaaattgga agggagactt actctctaga aattcttttt ccaaaccatg
    34381 gggattaatc tttccttgtg aaaaaagtgc aggatttcac attgtaaccc aaggaaagtg
    34441 ctatattcga tataaaaaca aatctatttt aatgagtaaa ggcgacattg cgtttatcgc
    34501 aagaggattt acacatgagt tggcgtctga tccaaatcaa aaagcaatgg atatcaagaa
    34561 atttagacaa ctcattcaag cacaaatgga aacacaaaag attattacta gtattctttc
    34621 tgtgcgatat gaaattaaaa atacacctat gcatccattt tttttagaac tgcctgatgt
    34681 tctttttatt aaatcagagg aaattcctac ccatcatcct cttcagacta tattgatttt
    34741 aatttcacag gaattggaca ggggaattgg atcagatgta ttattagaaa aactttctga
    34801 tattttatta tactatgtga ttcgacattg gatggaaacg aatccaagtc attctcctgg
    34861 ctggatgtca gccttgaaag atgaaaaaat tttatctgct ttggaggcaa ttcatctcaa
    34921 tccatctcat gcatggacgt tagatctatt agcaaattcg gttggcgtat cgagagcgac
    34981 acttgcaaat aagttcaggg atactctcgg atcaacacca atggattatc ttgcgaagct
    35041 aagaattgaa aaagggaaaa atatgcttat agaaaaaaaa gctacaattg aggaagtcgc
    35101 acgcagcgtt ggctactcct cggcatttgc attttctaaa gcttataaaa gaataacagg
    35161 atattctcct aagacgtcta gagatacggc ggcagttgtc tagttcactt aacgctttcc
    35221 atccattcaa tacacttact taattgtgat ttcatcgtag ttttatcgga agaaaaactt
    35281 ctaccatgtc caggcaaaat agattcaaaa gtgaattcat tcaaacgttt catggaatca
    35341 atttgttttt cccacgaata ccaacaagca tttcgaaagg catgtaaatg accttttgtg
    35401 ggagagtagg ctaggtgatc cccggtaaat aaaaaactat ctttatataa gagaacacaa
    35461 tgtccctctg tgtgaccagg gactggaatg atttttatgt cctcatctaa atgaaaaatt
    35521 tcttctcctt ctattggaat ttctactgac gatagttgct ttgtaaaatc gtctttgtgt
    35581 aaaattcgtt ttgctccaaa gtaatctgca tacttttgtt gatctgcaat atcatcttga
    35641 tgtgtgagaa atatataatc tatccctcct agctttttaa agttttctac taaggtcatg
    35701 gcaaatcgag gcgaatcaat tagtatgttt cctttttctc ttttgataaa ataacttgca
    35761 gccccatacg atttttcaga atgatatcca ttataataaa cagtatctgc aatattttta
    35821 ggaaaactaa gtcgtatttc ctttagttct ggaagcattg tctctaaccc aattgagtag
    35881 gtaggacatg cgactaatgc ttgtaatcct ctttcaatgg aatcgtttgt agttggttga
    35941 aagtaaacga tagaaccatt tcctactttc ttaaaaattt ccggagccag aattcgacag
    36001 gtctcacaat ctatacaggt tgaatctaca taaaaatttc ccagaatatt ttcttttttg
    36061 cgtttacttt gaattgccat atagattaga catcattctt gaaaaaatct ataatcaccc
    36121 tgaaaggata caatggaaaa tcgattgatt ggatgaagtt tttagaagct agtaaaaaaa
    36181 agtatttgcg agacttttaa gaaaaaaaaa attattcgaa tcttaagcta aaaatcaaaa
    36241 gaataaggat ttgcatatga gattagttat tatgtttcga atttgatcta attttgtctc
    36301 tatctataaa aattcttttt tgatcagtca gtgtttgttt agcttcgtta tgttttaaga
    36361 aaataaaagg aatggaactt gcctgatttg catttagaat tcgattgcag gtatgagatt
    36421 acagaatgga cagtaaaatc caatcccaat acaaaggcta agacttgcat ctaggatata
    36481 tttataacta gaaatttggt tccaattttc tagatgaaat gatctagagt tggttcttca
    36541 ggataatgaa aatgagagag tggatttgct cttcttttca atttttggtt ttgaatcaag
    36601 attcaaatta tagaagcggt caaagtttta tctaagagaa aacttggaaa cttaaatact
    36661 tcagttttct cttatccaaa ataaaatgaa tccaaaattt tagaatgatt ataaaaaact
    36721 ttgtttcttt ttctatttgg aatcttaggt tggtctaaaa gtataacaaa aaggagatta
    36781 tattagaatg ccacaagtta catcccaagc ccctgacttt acagcagaag cggtcattgg
    36841 ctcagaattt aagaatttaa aattatctga ttacaaagga aaatgggtag tattattctt
    36901 ttatccattg gatttcacat ttgtttgccc aactgaaatt atcgagtatg atacaaagct
    36961 agatgagttt aagaaactag gtgcagaagt gatcggagtt tccatcgaca gtaaattttc
    37021 ccacttagca tggaagcaaa agcctagaaa ggaaggtgga attggcgata tcaaatatcc
    37081 actcgtagct gatattacaa agcaaatctc aaaagatttt ggagtattga ttgattctgg
    37141 agatgatgca ggtgttgcac ttcgtggaac ttttatcatt gatccaaaag gtgttattcg
    37201 ccaagctact gtaaatgatt tacctgttgg aagaaacatt gacgaagcaa ttcgtttaat
    37261 caaagcattt caacacaatg aaaaatatgg agaagtatgt cctgcaaact ggaatgaggg
    37321 cgcaaaaacc atgactccag atgtaaacaa atcaaaagaa tatttttctg cggtaaataa
    37381 ataataacac tgccaccgtt ttttggctta ggttgctaac accggtaaaa atacgatgac
    37441 tatcgttttc ttatcggtgt tcatcggtgg tatttctttt ttaaagcact aggagataat
    37501 aatgacaaca atcacaacga gtatacaaga agaaaatgaa agatactata gagcggataa
    37561 ctttccaaaa ggtttaactc gtaaagtagt tgagtctatt tcccatatta aaaatgaacc
    37621 tggttggcta acagagtttc gacttcaagc atttgaagta tatgaacaaa aaccaatgcc
    37681 cacatgggga tttttccccg aattcaatgt caatatagat tcttatactc actatgttgg
    37741 aagtaatcag aaaaaaaaga aatcttggga agatgttgat cctgaagttt taagaagttt
    37801 tgaaaaacta ggaattccag aacacgaaag gaaataccta gcgggtatag aggcaatgaa
    37861 tgattcagaa accgtctatg caaatgtgaa aaaagaatta actgatcttg gaattatttt
    37921 ttgtgatatt gatacagcta ttaaagaata cccagaactt gttcgtaaat atctaggcac
    37981 tgttgttacc attggagaca ataaattttc cgctttaaat tcttgtgttt ttagtggtgg
    38041 ctctttcgcc tattgtccta aaggagtgaa gactccaatg cctctccaag cttattttaa
    38101 agttacagca gcaagtagtg gtcagtatga aagaactcta ctcattgctg acgagggagc
    38161 agaaattgaa tacagtgaag gttgcagttc tgtgcaagat aaaggaacaa attttcacac
    38221 ggcagttgta gaactcattg cgcataaaaa cgctaaaatc aattacacta caattcagaa
    38281 ttggaaaaaa aatatgtata actggactgt gaagcgtgga ctctgtcatg aatctgctca
    38341 tatcacttgg acagattgta atattggtgc taatacaata aagtatcctg gaattgtttt
    38401 actcggagat aattcaactg gaagtgtatt gtctcttgct tttgctggtc atggtcaggt
    38461 gcaagataca ggagcacgta ttatccatgt tggtaaaaat acaagaagca atgtacttgc
    38521 gaaaggtgtg tcgcttgacg gtggagtaaa ctcatacaga ggtcttgtta aatttgaacc
    38581 ttcttctaag gatgcgttta gtcatattaa atgcgatggg ctcatgatgg acgatcattc
    38641 ttcttcccat gcttatcctt ataatgatat cagtggagag catggttctt taaattatga
    38701 agcgacagtt tctaaaattg atgaagacca gttgttctat ttgcaatctc gcggtttaag
    38761 tgaagatgat gcaaagctat taattgtaaa tggattttgt gaaagcatcg taaaagaatt
    38821 aaatgtagaa tactctgtgg agatgactcg tctgattcgt atgattctag aagatggtaa
    38881 agtcattgca gaagcaccta aggttgaaaa aatagcacag tgaaaacaat tcttaaggga
    38941 atgactcccg cccaattgga agattttgtt tcttctttag gagaaccaaa gttcagagga
    39001 aaacaaatct ttcagggaat atacaaagat aggctttctt cgtttgagga gtttaccact
    39061 ctacccaaga agctaattgc aaagctaaat gaagtcgcag aattgcccaa tttaaaagtg
    39121 agacgtcatc taacatcgaa agacggcact cacaaaatga ccttcgaagt agaagaggga
    39181 aaggaagtcg aatctgtttg gattccgtct ggagacgatg gtagaaaaac aatctgtatt
    39241 tcttctcaag ttggttgcac tcttgcctgt aagttctgtg caacgggact tctcgatttc
    39301 aagggaaatc taaaaacatg gcagatcata gaccaagttc tgcaagtaga aaagatagta
    39361 ggggatagag caacgaatat tgtatttatg ggtatgggag agccaatgca caattacttt
    39421 tccgtaatca atgcagctca tctgcttcat catccagaag gttttaattt gggagcaaaa
    39481 agaattacta tttctacggc aggagtgata acaggtattg atcgctttgt aaataatcaa
    39541 gaacctttta actttgctat ctccctaaat catccgaatc cagaagccag aaaaactgtg
    39601 atgggaattg atgaaaaata tcccttagaa aagctactca tcgcagcccg aaattttaca
    39661 aaaaaactaa atcgtaaaat tacattcgaa tatgtgatga ttcccggtgt gaatatggga
    39721 aaagaaaatg cgagacgatt agttaaaatt gctcgctctg taaattgtaa aatcaatttg
    39781 attccactca atactgagtt taacggttgg tctagaccaa cagaagagga agtagagcat
    39841 tttaaagaat taattaaacc agcaggagtt ccgattatga atcgaaggtc tcctggtaaa
    39901 gatattcatg gtgcttgcgg aatgcttgct ctaagaggtt aaagagtgca tcgtctctag
    39961 aacattaata ttgattgttt tgataatttc attttgattt tcgaaagagg ctaccttcca
    40021 ttttattcct gtattgctag aatagaaact cctaccgctt aacgtcttat tattaaataa
    40081 tctaccgatt ccagaggatc taacaatgtt tagggaataa gtttttgaat actcagcaaa
    40141 taggtctaaa tctttttgat attcggaagg ttctgtctct gattcataga tcatagaagt
    40201 attcagaagt aattctacct tttcttcctg tatatgggaa aggtatgctt catttttact
    40261 ttcatttccg attaaaattg caaatcgaac tccacctaaa atgaagatgg attcactttc
    40321 tcctgcggaa ataggaatgt cccctgattg ttctggtatt cttgcatcat accaatcaat
    40381 cacattaatg tcttggacaa taggacagga ttcataaatc tttccgttta cttctcggag
    40441 gattgttcct ccgatgatta ttcccttgta gtattcagag atttcaagaa gtttatctat
    40501 atagattttt tgattgtgca gactgctttt ttcactcact ttagccagtg taggaaagaa
    40561 tcttggtaaa atcaaaaaat cagatttctg agaaattaat ttttgcttct ggtctcttgt
    40621 tatatctgta tttatgtttt tctggaatat agtaatttta attatattca tttgctctct
    40681 ctttttctgg ctattaaatt agatatctcc catgtcctta ggaccggatg attctgtttt
    40741 tttcgctcca ccttccatta aattattaat acttaagtct tctcctgata aatctttacc
    40801 aaaagaggct aacatttctt ctggggttaa ttcgattact cccccataca aatctccacc
    40861 ttctgtcagc cttgttgcaa atgcataaga ataacagtaa gcttccataa tacattgctt
    40921 aacttgtttt ttgtggagtc tttttttgga ttcaattgaa tcaaatacaa ttacttcctc
    40981 catgttgcct acaatttctg caggtccttt catgtcttca attagcatgt tgaaggagtc
    41041 ataggatttc tcaatataat cttttattgt tactctaacg tttctagatt gttgacttct
    41101 cttctgttct agtcccgttg tctttttaga ggcttcgatg taattcgttc ctgggttagc
    41161 tttgtgcatt gcctgttctt taaatacatc aatgtagatt ttgaattgct ccatacaaga
    41221 acgagtcaat tcataaacat ttaaaagctt ttgtttatta tctacttttg ttccatttac
    41281 atttgatggt ttgatcgtta ttttcttggt tgttaataca aatgaatatt cataatcaaa
    41341 ctctttaaaa aataaatagg tgagaagtgc tttgtctgta tccggaattt cgatgagctc
    41401 atttctagga tcatactttc ttctaagttg ctcaatagaa acttcactca tcagttttct
    41461 accataagca agctcttggt tttccccttt tttctcattt gggttttcct ttttttcttc
    41521 tgcttgcgct tcttctgtgg attgttctcc tccagcatct aacggtaaat tttctcctgc
    41581 ttttcttttt cctggcttct cactttctaa tatattgaga agattctcca tataaagaga
    41641 taccatagga atatttttgt tctcgtttcg aatgatgata taatagagtt tttcaaaaat
    41701 tttatcaaat acgacagaaa tttcactcga tatttttttc ttcttagttg cataaatcaa
    41761 agccggtttg ttttcaattc tttgtaatac ttcgtaggca gtaaggacag cctttctgta
    41821 tgtggcttgg taagggtaga ggtagtaaag ttttttgaat atggaataga ctggttttgc
    41881 tactcgattg ataggaacag gtatatctgg attagatgag taatctgcaa atagatcatt
    41941 catttcaact gtatcataaa ttttatgggc tcttccgatg agctctacat agattggaga
    42001 gattcgatct aattctttag ttacttttgg tcctagttgt gggtttccta aaatatcgct
    42061 tccagcaatt ttaaattcta atagagcctg ttgaaattct actcttaact ctgacatgaa
    42121 ttttggaaga agatctttgt ttccgaatgt tgttacattg tttgcccaac atttaaattt
    42181 gataatgagt cgattcataa aattactcat ttccgcatcc attgcaccta tttgtgcttt
    42241 gctgatgctt acttcggaag agtcatcgtc atcatcactt gcgccaattc cacgatacat
    42301 accaggcgag aattttttat caccactaga ggattttgat ttatcatctt ttcctccgac
    42361 agctttcatc ctttgtgtat tttttggctg ttgttgtggt ttgtcttctt tatcttttac
    42421 aactttcccg ccggcagatt taaatttatt gaacatctca ttccgttcat tctctgccat
    42481 tccaccaaca ccgattgctt ttttagtttt atcaaattca gccattctag tttctattta
    42541 tccttaataa aataattccg acagaatcgg tttatttgct attactcgtc tatcccttct
    42601 aatgagacaa aaacaggaaa aacgagagtg tagcttataa taaaatttaa atcttattaa
    42661 aatcaatcta tttttatcaa aaaatttcca aatgctatta ttgattactg tattctgtct
    42721 cattgcaata tagatttttt ttatactaac gcaattttta taatctctcc gatggaataa
    42781 gagagggttt atatttcatg tcaatttata gtcaagcaaa aaaaaatctg aatcagattt
    42841 ctgttggcga gttctggact gcaaagcaaa gacaatctca tccaatccat tatgcggtaa
    42901 gttatagagc agcatttaag ccagaacttc cttccttttt tattcgagaa tatctaggga
    42961 ataaaaaaag agttatttta gatccatttg gaggaagagg gacaactgca attcaagcca
    43021 acttagatgg acattatgct atacacaatg atataaatcc tttatctgtt tttttggcga
    43081 agtctagaca aatagttcca actctttctg agatacagga aaaattatct tctctagatt
    43141 taaaaaagaa agtatctgat tcagagatgg atgaaaaatt agaacccttc tttcataaag
    43201 acacacttag agaaataaaa aatttaaaat taattgggaa agaagataaa tcacatacta
    43261 tgagttatat cttattgacc gctctctcca gattgcatgg acattccaaa ggattttttt
    43321 ctgtttatac atttcctcag ttttcgattt cacctgagtc acaaagaaga aataatttaa
    43381 aacgaaatca aaagccagaa tatagagata ttaaatctag aattttagca aaagcaaaaa
    43441 gagatttaag taaaagtctt cccccctttt atcatgaatt ttcaaagttt aatcaatata
    43501 caaatcataa ttcagagcag atgacaaata tcggaacttc aactgtagac tttgtaatca
    43561 cctctccacc ttttttagac aaagtaaatt acaaagaaga caactggatg aaatgttggt
    43621 ttatggacat cgattcagag tgtatggatc aaatttctat cctacactca gttgacggtt
    43681 ggtctaaatt tatcaaagca tcgttaaaag aatgttcgag ggtgatgaaa aaaaatgctc
    43741 atttagttat ggaagttgga gacattcaga atggtaaatc ggttgtaaat ttagatgatg
    43801 ttgtaataca agcgtcagct ggcactggtc tttcttggga gaagacttat atcaatagtc
    43861 agaaatttac aaagctttcc aattgttgga atgtatcaaa taatgagaaa ggaacaaatt
    43921 caaatcgttg tgttgtgttc cgaaatgtta agtaactaaa atactgataa tgctgtccca
    43981 aataaatcat ttgcggcgct tccaagtatg gaagtgcttg catttgcaga agtcgtagcg
    44041 gttattccag aattgccgct tgagtgatat atataaattg cgcctgtatt ggtgcctgca
    44101 ttgtatcctt ttgctgcaat cactaaatca gcataaccat cccctgttaa atctccaaca
    44161 gaggtaaaat atccaaatat atcagaggaa gcattcccag atatcgttgt atttgcgcta
    44221 ttcacatttg ttacactaat accactacta ctggaagtaa atagatagac tgctcctttg
    44281 tttgcccctc cagagtatcc atttgcagaa gcggctaaat caccgtatcc atctccgtta
    44341 aaatcagcta tgctcactga gaagccaaag gagtcatttg gagaattgcc aaggatgctt
    44401 gtatttgcgc tagatacatt tgtggctgtt attccacttg cgcctgtcga tttaaaaata
    44461 tacaccattc ctttgtttcc agtcgttcca atggctccta cgatcaaatc cgcatatcca
    44521 tctccgtcaa tatctcctaa acaaatcgat gttccaatgt aatcacccga agcacctcca
    44581 atgatttttg ttgaagccga attgggattg gttatggtaa ttcctgaatt accgctcgag
    44641 tgaaatacaa aagctgctcc tagtaagctt ccacctgaat agccattggc tccggctgct
    44701 aaatccgcat agccatctcc attaacgtct ccggttgaaa taaatgttcc aaagttatca
    44761 aagttacttg gtccggtaat atttgaattt gctgaaccga ctcctgtagc agttattcca
    44821 gaggagccat tggaatgaaa tatatagact cgaccgttat tgctcgagga agggtaataa
    44881 ttagcggcga ttactaagtc tccgtatcca tctccattta tatctccaat ggaaacggct
    44941 gatccgaaag tatcattcga atttgctcct gtaatggagg catcggctgc tgcagcagag
    45001 tttgctgaaa ttccattcga gccattggat agaaaaatat aggtaactcc tttatttcca
    45061 acagcagcta ttgctcccac tgcaatatct gcataaccgt ctccatttac atcacctaat
    45121 gcaatcgagt atccaaattt atctccattg gtttgacctg aaatagtttt tgatgcactc
    45181 gtcgcacttg tcgcggttac tcctgaattg ccgctcgaat agaaaatata gatagctcct
    45241 ttatttaatc ctccgctgta gccggttgca ccaactacaa catccgaata tccatctcca
    45301 ttaatatctt tattttttcc tttccgaaca atgatagagg taatagaaga ggtatttcct
    45361 gcggcatcta cagatcgagc tgatattgta tggctgcttc cttctttcca gatagaagaa
    45421 cccgttggaa gttgatagga ccaagtgttt gttccagaag cggatgaata agttcctgaa
    45481 tcaattgaaa tttctacttt tgaaattgtt cctgttgctg ctccaattac aaatccagtc
    45541 tctaaaagac ctttgtttag caaatttgta aaactaggtg ctgctactgt agaagttgta
    45601 gtgccagtgg ttgcactcga accagaactt aagccagtga gagattgcat gaattttaat
    45661 tgagtctttg agttcaagtt acagctttcc attataaaaa atgcagataa aaaaaataaa
    45721 tacaagcttg ttctgacttt acagactggc aaatccatat taccctcaaa aagacctttt
    45781 gaataggact gtcaataatt tatgatttaa gtattccttt ttaatcgata gaagttaata
    45841 aagctttctg taacttgtgt attcgtagag ttattcggct tatgtgtatt atccgcaaaa
    45901 tttttctact ttgtattttt caatttgatt taacttaatt atgattcctg cctatgactt
    45961 acgatttaat tttaactatt tgctttagtg aatcaattag gggaatttca ctttctctta
    46021 ttttgaagtc ttggaattta agattgggtt tttgtagttt gtcaattcta ggaatattct
    46081 ctaggtaaag attgtcataa accgcctcag taaaattcat aaactgagaa aagtaagata
    46141 gttctgaagg cattttaaaa ggttaatcaa ttcataaaat caagtttctt gctagtatgt
    46201 aaaaatcaga cagatttttt aaacctgatt tatatttctt tgtttacctc atcagaatca
    46261 aaaaacaaaa attcctatgg tttaaggttt aggaaggtgg gtttaggagt cttttaaatt
    46321 cctttaaggg cgatcattta gctaaaaatg agatctgttt acaaatccaa cttaaaacca
    46381 ttctccaatt ggtgctaatt cctttcccgc tagttaagtt aggttaagat ctatctcgaa
    46441 gtgcgtttag tgccttaaat tcaattttac tagaaaatag ttctactgtt tttttcagaa
    46501 tcgccgacag taaaaagaaa agaggtatac tatttatgat gcgatctttg tggacagccg
    46561 caacagggat gaatgcccaa caatttcata tagacacaat ttcaaacaac cttgccaacg
    46621 taaatacgac tggattcaag aaaaatcgag ctgattttga ggatttagtt taccaacacc
    46681 aagtattagc tggaactcca gcaactgctg tgagtgaaat tccaactgga gtaaacgtag
    46741 ggcatggggt aagagctgct gcttcccaaa aattatttga gatcggatct tttcaagcta
    46801 ctggcaacaa gctagatatg gcaatcactg gggaaatggg attttttaaa atccaaatgc
    46861 cagacggaac ctttgcttac actagagacg gttcttttaa aattgattcc aaccaacaag
    46921 tagttacctc aaatggttat ttatttgaac ctccaatcgt tcttccggaa aatgcgattc
    46981 taaatactct ccaagtcgca gaaggcggag aagttactgt taaaattgga aatgatatac
    47041 gcccgactgt agtgggacaa atcgagcttt atcgcttcgt aaatcctgct ggtctgcaag
    47101 caattggtaa aaacttattt aaagaaacag ttgcttctgg tcctgagatt gctggtgttc
    47161 ctggaacaga aggaatggga gagattctac aaggcttttt agaaatgagc aacgttaaaa
    47221 tcgtagaaga aatggttgct atgattgttg cgcaaagagc ttatgaatct aactcaaagg
    47281 caattcaaac ttctgacaat atgctagcta ctgcaatatc tctgaagaga taatttgcgc
    47341 tgatccatgt tcaaggggca caggattaaa gtttctatcc aaacttttct tgtgcctttt
    47401 ttatttctat ttatctcctc tattcaagcg aacggtttat atttaaagcc tagaactatt
    47461 attgaaaaat atccaattaa actaagcgat gttgcccgtt tacacaaaga tataaaagat
    47521 agggttctct ttgattcttt agaagatata aaaatcctaa cgattgacga attgaaagat
    47581 agtataaacg atcctaatat ttccttttac ggaaaagaat gtttgatcat tcctttaaat
    47641 aaaaaatatt ccaaggaaga aatattagaa tctttagaaa aggaattttt ttcaaaactt
    47701 ggaattcaat ctacagaggc aaagattcat tatctaggag aagaggaaac ctttccttcg
    47761 agcggagtag agcttcgttg gaataactta ccaaaaagac taacgcctgg acaaaagata
    47821 ttttctcttg atttttatgt agaaagagag aaaatttatt ctaagaagtt gaaattttta
    47881 gttgaaacgc ctttgactgc acttgtggca tcacatgata ttgcacgcaa tgcaaagttg
    47941 acaaaaagtg attttatgac aaaaacattt ttttccgcag atgcaattgc ggatggtttt
    48001 aatcatgata ttacaggata cactgcgata gttgcaatag ctgaaggttc tgtgattcga
    48061 aaaaaacagg taagagaaat ccatttagta gaaaaaggga tggaagtaga tattgttttt
    48121 atgaaaggtt ctttatttgt aaggggaaaa ggggttgcta agaattcagc caatgaggga
    48181 gagcttgtaa aagttgtaaa ttcatcatct aaccatacag tctcaggaag agcaatggac
    48241 aaaggagtag tagtaatcga atgaaaaata gatacaatca ttttaaaaaa caaaatgcca
    48301 cggggtttga aaattacaaa agagcagcga tccatcgatt cgccatggtt aaaaatctgt
    48361 gcttattgtt cattatcttt cttttatcgg tggtaattcc tttaggttca caatctcttt
    48421 gggtggataa aaacccgtat gcaaatggac aagatattcg gttgggttct gtgattcgtg
    48481 tattaatcaa agatggaatg aaaggcgatt atgtttacga aggtggaaaa gacgattcct
    48541 ttgtgataag atctcatcct gataaaaaga tagtagatga aatgaaagga tttacagcag
    48601 acagaagttt tgcgcacaga caaaatggaa aatcaaaatc cactgcaaag atcattggag
    48661 caatgtctgt gcttgttacg caagtagatc ctgtgagcgg cttactcaca ttagccggaa
    48721 cgagggaagt tggttttgac aatgcaaaaa atagtctaaa actttctgga atcatttctc
    48781 ctttagatgt gagagacgat aagaccgtta caagtgatat ggttgcgaac ttaaaaattg
    48841 aatatgcaac agcaccgaat aaagaaagtt taaacgaacc ggatattcaa ttaaaaacaa
    48901 agaaaaatgc aaatggaacc gagaccattc aaaaagcaga attatccaat gaagaaaaac
    48961 aaaagattat acttaaaaat atgaaacgtc ttttaggaga gagccaatga aagcaaataa
    49021 aagtttaaag aatacttggt tggttcgttt gagggagttg ggttatttcc cttttaattc
    49081 tatcttagtt ctaagtttat ttttatttca aatacaaagt attttttctg ttgaagttag
    49141 attgaaagat atttctagaa ttcaaggaat gagagacaac caattgactg gctatggaat
    49201 tgtaactggt ctttctggaa caggggatac aaagagcgct gtaacaaatg aagcaattaa
    49261 aaattatcta aaaaaccaag gacttggaaa tgcaaaaggt gcgaacatta caagaaatat
    49321 tgcatccgta ttaattacag caataattcc ttctcatgca agagtagggg ataagattga
    49381 tgttgttgtt tcgtctatcg gtgatgcaaa aacactagaa ggtggagttc taatgcaatc
    49441 tccactaaaa ggtgcaaaca atgaaatcgt agcagtggca agtggtgttc tttcttttgg
    49501 tggaaaggat aacaatcgtt ctgcgcagcc ttataatgct acttcttatc tctacggtaa
    49561 tactgcaaat gttttcactg gaacaagtca tgctaaaaat tctcctaaaa cagtcggagt
    49621 gattttaggt ggtgccattg tagaaaaaga aatcaagggt gatttctttc aaagaaccaa
    49681 taaagaggac caagaaaata gatatcgaat catacttgag aaccaagatt ttgctacttt
    49741 aaatagtgta agacagcaaa tttcagacaa aataaaagta gaaactactg ctgtttctcc
    49801 gacagagatt gagattgcaa ttccaaagga gcaagatcct gtttctatcc tagcgcaact
    49861 ggaaaatcta accgtaacac cagatggaaa ggcaaaagtg gtaatcaatg agagaacagg
    49921 caccattgtt atgggggcaa atgtgaccgt tgacgaggtg gctatctcaa aacagggaat
    49981 taatgtaatt atttctaata aaaaaaagga ttctgacgat actaaagttg agttgatctc
    50041 tatgatcggg gaaggaactc gtgtatctga tatagtggat gctttaaata aaattggggc
    50101 ttctacaaag gatataatag ctattttaga aggaatgaag aaagcaggtg cccttcatgc
    50161 agaggtgatt gtgcaatgaa cgatatacaa aatacaattc aattcaatcg tttgaacttt
    50221 ggacagaaaa ctatggacgc tgttcagaga atcgatgcaa atcaacctaa atcttcactt
    50281 agttttgaag aaacgctacg agcagaaatc aatccgaaca ttcaaggaaa agtttctagc
    50341 tcttctattc gccttcctca taatatcaaa aaagaaatcg aatcagatga atatcgaaaa
    50401 aaacttttcg atgcaagcgt cgaattcgaa tctatcttcg taaagatgat gctttctcaa
    50461 atgaagaaat ccgttcaaaa atcgggaatg attgatggcg gttacgcaga agaaatcttt
    50521 gaagacatgc tctatgatga gtattctaaa aaaatctcgc aaaatgaatc tctcggaatt
    50581 gcagaagaaa tctatcgcac tctctcccaa tctcttccca aagtagatat aaaaggttaa
    50641 tccctttttc ccccctttgc gaaacatatc cttacccata agtatgttaa aactgactac
    50701 cacctattgc cgcgaacgag cggcaatgga gttt
//
LOCUS       DAOQOS010000026        50419 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_261_length_50419_cov_6.188786, whole genome shotgun sequence.
ACCESSION   DAOQOS010000026 DAOQOS010000000
VERSION     DAOQOS010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 50419)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 50419)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50419
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_261_length_50419_cov_6.188786"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..350
                     /locus_tag="PLF66_07715"
     CDS             <1..350
                     /locus_tag="PLF66_07715"
                     /inference="COORDINATES: protein motif:HMM:NF014207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HMZ63964.1"
                     /translation="KELGKMTLNIDVVQERVEGRILVDSEMVKNRLLGDVSQLKAELK
                     ANGLELQNISIEVRNESTQAFEFANASSDKDSSRENGSSSSNGSLSQNQTEEMEVELG
                     YTRRSNNLVDIKV"
     gene            359..1078
                     /locus_tag="PLF66_07720"
     CDS             359..1078
                     /locus_tag="PLF66_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011670697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook capping FlgD N-terminal
                     domain-containing protein"
                     /protein_id="HMZ63965.1"
                     /translation="MPTPPISGIKPKSIDPSTESTKTKYLDADKKVDIKSYLDKLSKE
                     EKSGLKGIEVRETPKQLGKDDFLKLLITQLSQQDPTNPMKDQDFIAQMAQFSSLEQMK
                     NISAGITRMEAKQSYSLVGKVVSGPDMVTGETVVGIAAALFFDADGKSYVRVNGRSVE
                     VEKITLISDPSVLNQEKEISTNNIKENQIEKKTSIQENQNLSDIGTAKQSVHETIQSL
                     DKPVDWNYPGAKTNKGTNYEK"
     gene            1106..2497
                     /gene="flgE"
                     /locus_tag="PLF66_07725"
     CDS             1106..2497
                     /gene="flgE"
                     /locus_tag="PLF66_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010575599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HMZ63966.1"
                     /translation="MMRSLYSGVSGLKNHQVRMDVIGNNISNVNTHGFKTERVTFQDM
                     ISQELSAAMEPKDNIGGVNPKQVGLGSLIAAIDKIMTQGSFQTTGKNTDIAISGEGFF
                     VVKDGDKEFYTRAGAFNVDKNGYYVNPANGLKVQGWNARVDENGNKYVNTASSVEDIV
                     LPLYSKEPAKATKEVIFQSNLNASVLAVDEGSKPEDIKRYINDPDPKKRRGHQTSIIV
                     YDDQGIEREIKMEMYKIRDNVWRASVSMTDATQVSIDVATKDQNTTVPGNTEIELSFS
                     PDGRLVGVSDGVDSLSSGKLNAEVSFRIAGNPEKQSFSLNLGESGMVTGITQFASDFT
                     TKAIKQDGYPMGYMESFSIDNSGRIVGVYSNGIQQPLAQVAMALFTNPAGLNKAGDTM
                     YSYSINSGEPNIGESGIAGRGKVNAGILEMSNVDLSDQFTDMIVTQRGFQANSRTITT
                     SDQMIQEVLGLKR"
     gene            2683..4653
                     /locus_tag="PLF66_07730"
     CDS             2683..4653
                     /locus_tag="PLF66_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002979665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HMZ63967.1"
                     /translation="MQLTQFVISDLPDLESKVSSFAKQINAVYFQLSQAHSFPEHSER
                     FIKVVFYLKKETLQNSQKEIRSLLKYNPNILALLVVNAPPEENIFETTDLENDLFFSN
                     IQDSVPAVFLSKTLVNAFSFLQLRLESFELQYKVNLSTSHISKLTRIGVSLSNEKDFT
                     KLLRDILYSAREISVADSGSLYLIDKDEKGNPKNLRFKISAMDLNSSEFILPINKQSI
                     AGYVAFTGKVLNLPDVYNLPKDVEYSFNRDFDKAKNYHSKSMLVVPMKNYLGEVIGVI
                     QLINRKKNFNSKLSVEQMRGNDVMPFDKYSEELVMSVAGQAAVAIQNNNLIQDIEALF
                     EGFVTASVSAIEARDPTTSGHSFRVALLTVGLAETVDRISVGKFSNIKFTNEQLKEIR
                     YASLLHDFGKVGVREKVLVKSKKLEPYELDLIKWRFHYMVKDLQERFTARKLDYVRKN
                     GNTGFLEFEKALEMEYAIEKKKLDDMLQVIITSNEPTILEQSNSQVLEDISKMNFSFL
                     DGSERPVLTSAELNYLSIRKGSLDFEERKEIESHVEHTFQFLSKIPWTSGLKMVPSIA
                     HAHHEKLNGEGYPRGLIANDIPIQSKMMTIADIFDALTDKDRPYKKAVPLERALDILK
                     MEVKDHHVDGDLLNIFIESRVFERLNTNKQIS"
     gene            4663..5502
                     /locus_tag="PLF66_07735"
     CDS             4663..5502
                     /locus_tag="PLF66_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015677899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HMZ63968.1"
                     /translation="MSNQPLQNCNILITGITDLGSLALAAARQVKKEGANLICTGLGL
                     TPYHSNLSDKAKAYLDKTYSDFKETIAKEFGDDTLTLPLDVTLDGSLEDVANTLKAKG
                     IKLNGLLHSIAMDKTIRAGAVKPLIEVSREEFMGAMDVSAYSLIALTRSLLQKEVLQK
                     NSSILSISYLGAEKIVRHPYKNVGVAKAALERITRELAFELGKSHQIKVNSIRFSPFT
                     GSKAGGAIQGLQEAFDFAEENAPLGNAIPEDLGYEVAYLLRPYGRITGEIRHVDGGYN
                     IRG"
     gene            complement(5646..6242)
                     /locus_tag="PLF66_07740"
     CDS             complement(5646..6242)
                     /locus_tag="PLF66_07740"
                     /inference="COORDINATES: protein motif:HMM:NF024769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HMZ63969.1"
                     /translation="MPTKNKEGKENSFHAKSKKEWRQWLEKNHKKEENIWLIIYKKES
                     GVKSVYYDEAVNEALCYGWIDSKINKKDEISFYQFFSKRNPKSNWSKVNKEKVERLIK
                     EGLMTEAGFNSIELAKKKGTWNALDEVENLTIPKDLEKALKSKKLAFENFQAFPRSVK
                     RSILEWILNAKKEETRANRIEETATLATKNIRANQYRQ"
     gene            complement(6226..6918)
                     /gene="tsaB"
                     /locus_tag="PLF66_07745"
     CDS             complement(6226..6918)
                     /gene="tsaB"
                     /locus_tag="PLF66_07745"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HMZ63970.1"
                     /translation="MNRPRDIKIQNESVRILFFDTSCDFILVGLYSLQNKEIKEEYFY
                     AGNHPRESSYRLLSDIQLGLTSVSWDKPDVVSCAIGPGSFTGIRISVATARNLSQLWK
                     IPNLGFDTIEMYSNFYSDFYKSPVIAVLDGKQKKVFAGSFRNETYKGTFDISPSEFEN
                     QFKEELDTENRIIIADTNLVKHDFTIESLLPNPISILKRNISKLEGLTLADNSYLTLL
                     PKYMRNTYAHKE"
     gene            7548..7751
                     /locus_tag="PLF66_07750"
     CDS             7548..7751
                     /locus_tag="PLF66_07750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63971.1"
                     /translation="MPQSPLVSEGCINNRVSSGLIFDVDVNQNISVDTLTSGKRFKCL
                     CNGNIGKYGYSWKRSKELFDSMP"
     gene            complement(8020..8511)
                     /locus_tag="PLF66_07755"
     CDS             complement(8020..8511)
                     /locus_tag="PLF66_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63972.1"
                     /translation="MNKNLNRILLALTIFTLSIGCIQTKKQNRSGLTALVLRNWASGT
                     ATDRYTNGSLVIMQRADLSNLSFTGQCFDTFTLKGIPVSPSNYYNTPAGGGGGLLNND
                     YKKYALSTSNCTSLGFTGTTNTNLGSSSQRPDPDQNFIFKMYTCDPNNNPCSRSAITA
                     SGF"
     gene            complement(8522..10231)
                     /locus_tag="PLF66_07760"
     CDS             complement(8522..10231)
                     /locus_tag="PLF66_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020776282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63973.1"
                     /translation="MKQKNLILGVLLLFSFTIYAQNETKTTEPNTATETDHAKDHAPK
                     PASKGLKEVYEDNETGQLFTKPGPGRTKVEPQKLSTTLPDAFAHRPDDTSKEKLTVYG
                     RIQFRGVTGSEQSPYSNGNNDFSALDWNFRRLRLGAMYENDWWGMNIQLRMENMVNRP
                     NTVTTTTNVVTGVSNNGGVITTTTTPVVNTVRMTDNRGYVHEAFVYAKHPYAGLRLTF
                     GQINTQFTREYLQSSANFITLERSIITNALPQFDLGVMLSANPLKELGHKWERYLQVS
                     FMIGNGKGAGGDYGTGRRQDLTTSNRWGTTLISPTYYGRIQYNVFGGLKRAVDGKEVN
                     WQEGEEVFQSDMKWSIGAAVMQTANFQPNTLAVPEYTPGTTNAFNLLNGQQSNPDRGT
                     TFSSTVNNIAMTLPNYNVQNNVTTPGRPSFGLVGHTYDTTFTYQGIYLTGAFTKFTGA
                     ASNNLHAWHTTVGYNLKFWDKYYIMPVLKYEQIMGDFHRDGNSHNQNDILRVYWAGLN
                     LFGDKHHFKAQLYYQVLGNKFDVNPNTGNYAAIDDRRIYFQLQGNFWTGTVSPEAYSY
                     RPN"
     gene            complement(10635..11540)
                     /locus_tag="PLF66_07765"
     CDS             complement(10635..11540)
                     /locus_tag="PLF66_07765"
                     /inference="COORDINATES: protein motif:HMM:TIGR02122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HMZ63974.1"
                     /translation="MIKKILGLTLITLVMLHCKSKKEQTLFAGGFQGGTYDTMAKSLE
                     ANQSLNVKVIPSNGSLDNINLLLDKKADFCLTQIDMFYSAKLGNPAIQKGINVLLPVL
                     QDEIHLIVNPSIKTIKDLKGKKIAIGHSESGIKATSISVLRISGIDFSEVDVKEIGPE
                     EALPLLLKNEIDGLFIVSGLPVKILSALKEDAASKLKILSLDESVTGQIKDDNKVYQK
                     STIPALTYSWQKEAVSTLQVQTVLLARKDLSQKEISDFIENLFKNIDSLSAAHPEWNG
                     IRKETLKEKIKTLPEFFHPIVKEKL"
     gene            complement(11728..13716)
                     /locus_tag="PLF66_07770"
     CDS             complement(11728..13716)
                     /locus_tag="PLF66_07770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63975.1"
                     /translation="MKKFLLTSFSLLALSFCIDVKKSSTNEATLLLLAQPATYQVSAN
                     ISTTSATSNAMVYFSPATTTTSSSVREASSTTTTSTNSTTSTTTGPVFTTSAQTDTNG
                     NVVFTLAVGEYQAVVTTTDNKGMAFKIKIESGSSLGLNKDGKATITYSDGSIKVVIIR
                     VSGNILNAIPNAPISFVCGYNPKGDTAAPELTSVSIDSSTVDVSSGSGKLRISAKVSD
                     GYEGTSSDKRATGVKSVTAKLFSPKRITGSGYTAHATLTLNANTGFYEGDATLTNFVD
                     NGTWNVGLITARDVTGNERQYLYDSSKSTTNYTFTGCGQKIDSKITIPTFTVKGSSPD
                     TAAPTLGTVTLKSDINSGATVQNPIASNVDISVSSASPKEVVITVTVTGATDTGGTGN
                     ASGIAYMDARLNSYSWWSANSNYLGNAIYIRLDLTSGTNTNGTYSGSATIRQFAEGNT
                     APDGQWFLGGIWVTDKAGNSLWTSRTTLNKSFTIIGASTTATADFYPPELKSISINKT
                     DVNFDQSFTISADVADGTTETTSADQNATTSSTTLTTNNSSTNASGVKAVNIVLYSPL
                     KLLDNTLGTTKTLSTTYNTSTLKYEATGTFPTASNEEGGTWKVGWVEVVDKAGNYRVY
                     RLSEGYTYYTYVKLTRVDGDVSGSFIVTNISPVAITRN"
     gene            14236..14880
                     /locus_tag="PLF66_07775"
     CDS             14236..14880
                     /locus_tag="PLF66_07775"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HMZ63976.1"
                     /translation="MVHFEKTQKNSTPWRIVVADDHSVAHFGIEAVLAANPEFTIVSK
                     ISDGRQIKITLQQEEADILILDVDLPELSGIDFLREERSNFPNLKILLFTVHEGEGFF
                     REALRLGANGYFLKSDHILNMPKALSCLVKNEFYCSEKIKSYLLKTEKSDELSSKEIE
                     ILKMVVAGFSAREIGERMEISRRTIEYYITRLKEKLDAENIVELVVTAKEKYFL"
     gene            14882..17194
                     /locus_tag="PLF66_07780"
     CDS             14882..17194
                     /locus_tag="PLF66_07780"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HMZ63977.1"
                     /translation="MKIVQVLILLLFSYSSQYAFDRVDLPTKGSSHLTEKIHYFESDS
                     EDLIPEIIKESKSFPWQKSNLSILNFGFTKKNIYIALNVRILGNENQYLVINTPRIDR
                     ILFYSPAKGVYRESRAGILDPLSVREFSHRNPIFLLEKTQEEETIVFFHIKSETSLQF
                     ELISYDHKSLLETTASEQIYFGIFIGVAILSVLYGLMLFHLMKDFANLYFSIYSLFSL
                     ITILIYTEFGFPYLWANYPSFNHFNLFSILLSAIFGVLFYRNVLNIDKNSPVLNRIFN
                     FKLIVLLITFPLLFFIKFSLSLKIVLFEIVLVMPIILVSGFKISLLRYKPGVYVFLSS
                     TLWYIEFLVRVLEYNQFLAFDFSFLDTLLFRILERVILCIALMERVRAIKNEKEQIQK
                     SKLEYEISVQKELSLLNQNMESLVKERTLELEKEKEAALHANLLMNSFVTTVSHDLKS
                     PILNIRNLLDIMIVKEEMDWEERIYYLKNCRNALTSSYQLVNNLLNVERFESGNLEIT
                     YQYLDINTILKETIQELAFILNAKNIKIEFQEKEDMIIIGDRQLVLQLFLNILSNAIK
                     FSHENGKIQILSQFNQNYYEIIISDEGIGMSQADVSRLQGNSIYSEKGTKGEVGSGFG
                     FGIIKKIVSLHDGKIQLKSKKNIGTEIQISLPCQDKVAICIWDLPLDYSILREKAECN
                     SIRLLRIFDLNKVTKLLETISPDFFVIGLSFRGFESISKELLNLLKFLENKSMQVYLV
                     SSRDVFEKIKEQKNLLFVISENKNLENIFV"
     gene            complement(17191..17979)
                     /locus_tag="PLF66_07785"
                     /pseudo
     CDS             complement(17191..17979)
                     /locus_tag="PLF66_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004755177.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YHYH protein"
     gene            18841..20397
                     /locus_tag="PLF66_07790"
     CDS             18841..20397
                     /locus_tag="PLF66_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020768094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HMZ63978.1"
                     /translation="MGEELIKKPLAGGGTDRILVQHSKDRMTVWERIKVLTDKEPTIF
                     YQNWGANLDGASLVTGILNIGGRDVAIYGHDFTLRAGSMDATNGSKLARLIYMAGEHG
                     IPLIGMNDSAGAFVPAGVGGLDGYSEAFTALRKISGKVPSVMLMFGFNAGGGSYLPRQ
                     GSFMIQCDNTFFGLTGPGVVKSVLGEDISAEDLGGPKVHGQSGVVDLVTPDEYGSLKT
                     ALRLLSYLPDNNGVNAPFFPTSDPLDRFTYEEDILFKKTFNSPTGMNTPFDITLYMQQ
                     ICDHGSYFEVQPQRARNLITAFGRIGGHVVAFLANNSAVSSGQIDIGAARKGTRFVRF
                     ANLYNIPMIFLEDTTGFLPGREQEAGGIVLEGRKLLDSIIDLRTPRITLIIRNAFGGA
                     YATFNSYFTGADVVYTLPTARIAVMGPAGKDYVYKDEISKINKAYAEAIKKGGAEDEA
                     KKVRDAELAKLSQLYEKELMNPKEALSLGSVSSVVIPGTSRKVLGNSLNYLMSRYKAS
                     PMGGVQREFE"
     gene            20397..23156
                     /locus_tag="PLF66_07795"
     CDS             20397..23156
                     /locus_tag="PLF66_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004472501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HMZ63979.1"
                     /translation="MFDVTGNKMKFHESSSEWIRSFDLSGIKCLIVCRGPVRKEAMDV
                     FDEIGIKEYGILLSEKDSVVYPMAYAPELRNFRFINNIHRISDYMGAGKEEKDRIIKQ
                     IINIAVTNNYTHIFAGYGFMAEDAEFIEAIENSGVKFMGPASSVAKQAGAKDAAKIIA
                     RKLGVSVTPGIDNITALTLLRKVGGKDGLAKIAKENYLNFSYDNNLSESENADKLLQL
                     SYDSHIDIITIEDLQKEAEIESEVIWKQYPNNRIRFKYIGGGGGKGQRVIKGKEEVKA
                     AVMEILAESKVTGVGSNRNFLIELNIENTRHNEIQLIGNGEWSLALGGRDCSLQMHEQ
                     KLLEISNTREMLEQEIASSKDPKRAETLKGDLKVLTEMEEQSERFGAAVKLDSVSTFE
                     SIVEGTNHFFMEMNTRIQVEHRVTEMAYKLKFTNPSNPNEFFIVESLIEAMAMLSVHG
                     KRVPKPQRILRNVSGAEVRINATNQALQPHAGGLIQNWSKPSEFEIRDDQGIGIRNPD
                     TGAFIHYRLAGAYDSNVALLVSYGNSRKENLERLRHIIKITEMRGNDLQTNMPVHYGL
                     INWILGHDVMFKPNTKFMLSYLAAVGSLASFLRDIDLEILWTETVKKTSKEDPALGKA
                     LAKKVTLITRPIKLLFNSPHLLAGCIGFNRHRSWKIVDGKPEFTRNPLEILKELYEYL
                     DLERSEGKAPCDEIWDHDEEILETGLAFYRDLQKISGKNEPYSFFDDALRNNKNPAPD
                     KIPADVWEKCMGSHAAFQVGLDLIKIIPKVGRASGFFEIRMNESTLEAEIPEKFTNAE
                     SVTKLVKFLAPPPKAKSDEIIAPMGGMFYAREAPNMPPMIKVGDHFNAGQPLFIIEVM
                     KMFNKIVAPFSGTITKSFMEDADGKIIQKGQPIFKVQPDEVIVEETEEQIYNRKKKAT
                     LELMA"
     gene            23342..24397
                     /locus_tag="PLF66_07800"
     CDS             23342..24397
                     /locus_tag="PLF66_07800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63980.1"
                     /translation="MKKIISIFLFAIAYSQLAAQAVKKDYLSYNWVKYFYSKQWNAYG
                     EEVTKQKQEAEKLNAEGFKLYKAKKDAEAIKKYEGAIDLFPSGEIYYNYGNSLSNVKR
                     LEDSVKAYEIALKLDTPRPELALYNIACSYSRLDKADNAYKYLGSAVQRGYNAFEYIK
                     KDPDMANLRKQADWETKLKAIKANANYDESALNGKITEEGPRQPEYFYLCKSGIALTV
                     GGDQLNCEGKGFDRGKWKLVNGDVKITFSETCNPEYVATEAERKKRETEPQYEGQCPV
                     GKPKFAACKKVDSKEYYSNRSFTREDIRPMLLPTSDDPAGMVGMPLLFSKFKNGQEPK
                     QCDPNFKVKELKDYKVE"
     gene            complement(24598..24969)
                     /locus_tag="PLF66_07805"
     CDS             complement(24598..24969)
                     /locus_tag="PLF66_07805"
                     /inference="COORDINATES: protein motif:HMM:NF037832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HMZ63981.1"
                     /translation="MKILIDMNLSPAWVEFFKEFEIESVHWTSVGNINATDKEFFNWA
                     KENNYIILTQDLDFSTILALSNSEKPSVVLIRAKSSLPEDLGNQLLKVLNLHSIALKD
                     GAHVILDTTTARVRILPLYEI"
     gene            complement(24966..25196)
                     /locus_tag="PLF66_07810"
     CDS             complement(24966..25196)
                     /locus_tag="PLF66_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016756384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HMZ63982.1"
                     /translation="MKQFSRITLNPSVMGGKPCVRGMRVTVGMILGLLASNKTNDEIL
                     KAFPYLELEDIQESLKYAAWRLMEFETELPAA"
     gene            25625..27673
                     /locus_tag="PLF66_07815"
     CDS             25625..27673
                     /locus_tag="PLF66_07815"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HMZ63983.1"
                     /translation="MDSKQLFRLGFMQSIRYILIPFIFGLLFLNCNKKNQSPDKIISN
                     IATIDSNFKTTNLTPILEYIEDKEGNLSFEEIQKREKDWLKLKNDEFTNFGFNPSFFW
                     IRFTVENITKDKQRPIFEISFPRQDYIEFYSETSGRYETEITGDIFPFHSRKINHLGF
                     AFELEIEANTKKTYFLRTRSIYNRSILNPHLHTQIEFHQRASERNLVNGILYGLMGIM
                     LFYNFFLYLTIREKVHLYSSVSIVFYLLITLGIHGTSYQYLYPDFPWIQNRDYTIFTP
                     LVTLFIVLYTNEYLDLKVTLPKVYKILKLFNAFTLGMAFTFPFLVEQFSKLIFQFILN
                     FCFFFVVFAFLLGAYLSVKRYRPAYFFMSSFSVFFVSIVIAFLTVNTFLPASFLGYWS
                     FQIGFSSMLILLALGTADKINYLKNNLKEANEFLEYKVDKRTQELKEAIAKIKIDLKT
                     ASKIQKNILPKNKTSIQKLKISTEYLPLEEVGGDFYDITELQNGVIRIFLADATGHGI
                     QAALMTMSIYSEYQNIKEYQLDAGEILNILNKQYYYRYYYLNAYFTCILVDIDLEKKK
                     IYYSSAGHPDQILFLENKTTLLKSSGKLMGVLKDVLYKTCEEDFREGSRLLLFTDGIF
                     EELNKEGEEFGEERFLEGATANRYLPNEEFPQKTIQSVFQFMNGSSLQDDITLLTIDY
                     "
     gene            complement(27706..27924)
                     /locus_tag="PLF66_07820"
     CDS             complement(27706..27924)
                     /locus_tag="PLF66_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002774254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2191 domain-containing protein"
                     /protein_id="HMZ63984.1"
                     /translation="MKVTAILPDQLINDVKEFSGGKNITESIEKALSEWIKLAKLKRL
                     NKTIQEKPLSFRDGFSAEKVRKLNRSNK"
     gene            28268..29395
                     /locus_tag="PLF66_07825"
     CDS             28268..29395
                     /locus_tag="PLF66_07825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63985.1"
                     /translation="MSELQQLDFKSVESILQSHSIEHLDMREISKLFINETSITNYRP
                     ESVFQIDPRNGELIVYNSSRATRPNVGPDGLEEKKKEAEHCPICEGKTTGIMDVTHMS
                     EGFSFINKNMFPIFYPHDTDVPGNMGSVSFGLHFLQWTSSYHHRDWHNISHKDAVIVL
                     SRLAALEKKLLVESSDFMPGSIASSQEKPTYGYVSIIKNYGKAAGGSLQHGHQQIVSS
                     NIMPKQFFNNWSFRERHGVHFSRFLMQENPEELTVKDYGKVVLVVPYFMRRPFDMFLI
                     VKDTSKKYIHELSQEELEGVAKGWQDATRIMLKILKRLRKEEAYNVTVNNGPGAGLYV
                     EFLPFTQLIGGYEKIGLWVCQESPFKAAETLRNFINELEPL"
     gene            complement(29396..30604)
                     /locus_tag="PLF66_07830"
     CDS             complement(29396..30604)
                     /locus_tag="PLF66_07830"
                     /inference="COORDINATES: protein motif:HMM:NF012927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase/NADH oxidase family
                     protein"
                     /protein_id="HMZ63986.1"
                     /translation="MPNLNRPLKLKNDLIIPNRLAKAAMSENIADRSLLPDKAIYRLY
                     ERFGSSGAGLLITGNIIIDKTALGEPNNVVLENATNLDPFKEWVRIAKQHGSILFGQI
                     NHPGRQSPVLLSKQPVAPSAIAVNAPFGIFATPRELREEEILDLIYRFTATALKLKEA
                     GFDGVEIHAAHGYLINQFLSPLTNQRKDKWGGSLENRMRFLMEVYRSIRAAVGENFAI
                     AVKLNSKDFQHGGFSEDESTEVAKTLDHEGIDLLEVSGGTYEKAAMTGVHEKDTTRLR
                     EAYFLEFAHLLRRTVSCPMMLTGGFRTRAGMDQATEEGIDVLGLARPIAMDPKFPSKI
                     ISGESMGMVLKRVTAGIGVVDEAGALEIAWYQQQLLRMGDGKNPDPNLSNWEAVSSLI
                     FKSGKKFFFF"
     gene            complement(30607..31491)
                     /locus_tag="PLF66_07835"
     CDS             complement(30607..31491)
                     /locus_tag="PLF66_07835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ63987.1"
                     /translation="MVQVDVFWAYGIGSTMAAAASKKLEEEKEPLSTSFYAKALVVLS
                     CIWAPTGMLLLLKHPSWETMQVAETLYSIPVWLIIGFGITNVTQGILGFWVTSELIKR
                     KKYYLSHLNWIFGYFGMFFILLYGWDGLGYDRFLYDRDLFGGEAWKPGAGYKGIGSVA
                     AMFTSTVAMTLYIDGVFLVPAILYYFSKWTKESVSTFSKKIVSSGALAANYLVGVFLV
                     GFGSAAISAITVYYTGLLIGAGDPFSRYAGTGGSTNMHILSYFLGLPISFAILYYGLF
                     KEGRPFHKLIKKMYHIEN"
     gene            complement(31506..32108)
                     /locus_tag="PLF66_07840"
     CDS             complement(31506..32108)
                     /locus_tag="PLF66_07840"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HMZ63988.1"
                     /translation="MTDIDKKKKDILAASVQVFAEKGFHAAGIADIARKLGMGHGTFY
                     RYFKNKEDIFHSAVNEILKEIVSIAINENPNGATNLEEYKEQLHRIGEKLFDLFSEDD
                     SMARILFFESQGVASSQKKISDSMEIFDEYTAQYLKNGINKGFLRKDLDIKITARAIN
                     AVIFEGIKEVSPIENKKQRIKASKEWITVIISIILQGIQK"
     gene            complement(32150..33517)
                     /locus_tag="PLF66_07845"
     CDS             complement(32150..33517)
                     /locus_tag="PLF66_07845"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HMZ63989.1"
                     /translation="MEKYYPDLRKRGILKISYTYFAINISRLLLASAGCIIAKFYSYT
                     AIFDISLLLVFTSLTYLLVETFFDLTNRFSNFSFFVSAIDLTIIYFFVYLTGMNSFFL
                     GGTIYATALCSLNPKIRQGLFCVLYCLFLHLLLLVLLFTNSIEYYNFLQEGEKINRFS
                     LILGFMLTHIVNILIYSIIIVLQRKLQKQNEELENERNKLQQSYNALNTDLNMARKIQ
                     NKLIPKANPYPFISFLYHPMMHVGGDFFDFVNFRDPNKLGIFLSDVSGHGVPAAFITS
                     MLKTAILQSGIRKENPAEFLFYLNEVLFNQIGGFFLTGFYCVYDIKDNSLLFSNAGHN
                     WPIIVEGENIRYLSGGRSSAIGIFQNHELIDRNKKYQNHEIKLNANSKLLLYTDGFTE
                     TRSISGDGIFFEENEMEKTLRNLSSLKGQTFLNELFLALQRFRGSNQFEDDVCIISLE
                     INNVL"
     gene            complement(33751..34395)
                     /locus_tag="PLF66_07850"
     CDS             complement(33751..34395)
                     /locus_tag="PLF66_07850"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HMZ63990.1"
                     /translation="MNAKLKVTIIEDKKYFCNHIMDLLSKHSRIEKVLCYHSVEECSE
                     KDLKESDIFLIDILLPKASGIDFLKTISQINPSSKKIMLTALDSEDTIFQCLKLGCIG
                     YILKSEAEKVSSIIDIIIEGGAYMTPSIAVRVMHYFQKQKKESKPENMEELSNRELQI
                     LKELAQGSSPSEIAALLLVSISTIRFHIRGIYQKLEVNNRLQMLRKAEDLGILE"
     gene            34992..36818
                     /locus_tag="PLF66_07855"
     CDS             34992..36818
                     /locus_tag="PLF66_07855"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HMZ63991.1"
                     /translation="MKLSIKNTIILSFATLFILILFMALLIIFLYASQNDLFESQQRK
                     FNSYLLADELRQSSDDLTRFMRTYALTGDPKYKEYFFSVLDIRNGKKPRPEKYDRIYW
                     DFYTVDGTKPRQDGETISLLNLMRQNGFTEEEFKKLDEAKKNSDALVHTEEIAMKAME
                     GKLEEEIRKMIQPNESLSEFAKRILHSDQYHKDKYRIMKPIDDFYVLLEQRTSREVLE
                     NKEKQTLLLRIILGTVVFLIIILISVSYTIHIKVTVPILNFTDEMGRLADSKDLTYSL
                     PASEKNEIGLLAKGFNGFLESILLLFRAFSTNINKVNSLSKTLDETIQETKRSIQEVS
                     VATDNVSSETGQLMVYTETIVNMVNQSKDKISFSSSISKDNTKNTNFLLEEITQTHRE
                     IEIANKELKEIDRELEQTAKVTEALSDNSKEINSVLRSVKEISKQTSLLALNAAIESA
                     RAGEHGKGFAIVADEVGKLAMQTNEATEKIGRVISEITNEIAGTVQKIRNSHASSQSL
                     IQIIQRIEKVISNNFEAVKESRTQTEKVSNAILVLEKNIFEVGEMTNEIFRANQHLTA
                     SGEEVSVAIKTRLTSLETIQEKMIALTKETANLQRDLNLFKI"
     gene            complement(37054..38685)
                     /locus_tag="PLF66_07860"
     CDS             complement(37054..38685)
                     /locus_tag="PLF66_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008596200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HMZ63992.1"
                     /translation="MNGLMMDFPLSLTSLLRRTIKNYPEKEIISYQSNNQVHRYKNKD
                     FYKRVLRLMNVLRNLGIKEGDRVATFAWNSYRHYELYFAIPCMGAVLHTLNIRLFPEQ
                     LEYIVNHADDKVIFIDHSIAKLIAPLLSKFPNVKHLVLMDDNTPTGVEIPNALDYEKL
                     LNEANEDIPLIDFPENQAAGLCYTSGTTGDPKGVLYSHRSFFLHSMGICFADSLALTE
                     RDRALPVVPMFHANAWGIPYGAAMTGSSLIFPGPNLGGPILAEIIEKEKVTLAAGVPT
                     IWKLLYQHLKSKKHDLSSLNRMVIGGSAVPRSMIENFQMEFGIPILHAWGMTELSPVG
                     TVSRLTSEMDSWDLEKQITWRAKQGMNVPCVEIKVEDDEGKEVDWDGKTVGELLVRGP
                     WIAKSYFNNENANTNFTTDGWFRTGDVVKIDEYGFMEITDRKKDLIKSRGEWISSVDM
                     ENLAMAIPGVLEAAAIGKPDPIREEVPVVFIVLTQEEIQKPENKRVNSKTVHDFLSKN
                     FAHWQLPKLSDILFVDVIPKTSVGKFDKKELRKRI"
     gene            complement(38682..39605)
                     /locus_tag="PLF66_07865"
     CDS             complement(38682..39605)
                     /locus_tag="PLF66_07865"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HMZ63993.1"
                     /translation="MIRFILICIFTLSSCTLFYKTIKPNYFPAPQKINSPEKLEFYWI
                     GHATVLIRFFDKWVITDPNFSSHTGIVVKRFIEPGIDLEKLEKVDFVLISHTHFDHLD
                     QPSLRKLIGSKHILAPSGGITYIPDALFENVISVENMKEFNLDGVKITAIPVKHFGGR
                     WLLDNFWDGEPYTGYIIEYKGVTVFFGGDTGYDDSIFKLIGKAYNIDIALIPVGPASW
                     ITGGGFGNSVHVNPFGAVQIFQDLNAKYMIPIHHSTFYRRGGSEREMILEALEKSGKK
                     ERIRLLKIGEKAEFKKDNANINFIKNEEIKK"
     gene            39702..40982
                     /locus_tag="PLF66_07870"
     CDS             39702..40982
                     /locus_tag="PLF66_07870"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HMZ63994.1"
                     /translation="MSEKHKAAISTYKKIYKTFIVTVILLIVSSQAITQFFIYSQKFD
                     SNRINIAGRQRMLSQNIAKTILKIHLDQNNPDANDLSELENLKNLFMTSDYVIQNGSK
                     SLNIDANQNDLAQQLFKSNKPHFDQIILVANTLLQDKKIDKDKIKTLFESEKEFLKNM
                     DLIVTAFNEESIKKSTRLQIIESLLAIFAVIIILIEIGVLYRPLIQTLIKDNEELSKQ
                     KKNLEEFSFVLSHKLRKHVANLLGLMNSINPNNLLEIREYFQYVRQSVYELDTVSREL
                     GDKATPYDLKIDMNSITIDSTKRFETIETLKTILLIDDDKITNILTKRILVQYNPQIK
                     VDTFTNPYEGLRFLEKLKENKGEFPIVFLDITMPEMTGWEFLDKLAGLNINPPVYILT
                     SSIDRRDIERARVYKNVKSFLTKPLTSEKMPTIL"
     gene            40979..41668
                     /locus_tag="PLF66_07875"
     CDS             40979..41668
                     /locus_tag="PLF66_07875"
                     /inference="COORDINATES: protein motif:HMM:NF016202.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HMZ63995.1"
                     /translation="MKYFLILLAWGIFGLAADEEILIQRFSTLVEKQNSDVKKNIALA
                     VEKLNGYKIEPNSTFSFSDIVGEASIQNGFVAGRVFYWNEAIYEPGGGLCQVSSTVFN
                     LLLLSGFVIKERHKHSQPVSYVPMGLDATIFYGKKNLRMQNPHSQSFRLVAEITDSTL
                     TFSLYGNQELLEKYELETEEEEHELPILKKEKSYRNAYTVSVFRKKYRGENLLETSLL
                     YKDFIPAVYFK"
     gene            41703..42068
                     /locus_tag="PLF66_07880"
     CDS             41703..42068
                     /locus_tag="PLF66_07880"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HMZ63996.1"
                     /translation="MQIDDEDRLTLKEEEDVTFIQIKGNIDLYTTPELKDAFDKLNLS
                     QKNKIIIDLLNVNFIDSSGLGILVTQALYLQKRKLVLKFINVNSTISHVFSLGGFTRS
                     FKRFSDKEEAMNSTWEFGV"
     gene            42050..42634
                     /locus_tag="PLF66_07885"
     CDS             42050..42634
                     /locus_tag="PLF66_07885"
                     /inference="COORDINATES: protein motif:HMM:NF013988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMMECR1 domain-containing protein"
                     /protein_id="HMZ63997.1"
                     /translation="MGIWSLIVSFFLTLTLWGEETALSEWKKFSKTKASEELMEFIRC
                     SATEELQLKNCDHPIPETPPFFGELGLFITIVNKGKVRGCFGAFRHKNTQITNVLQDY
                     IRGALRQDPRYPPLEIEELKTAYFILTIADSPAPVADTESIPLGRYGVLLTKENGQGV
                     VYVPGEIKSHSSLKRIMQKEKIQDVSIFKAILIR"
     gene            42648..48320
                     /locus_tag="PLF66_07890"
     CDS             42648..48320
                     /locus_tag="PLF66_07890"
                     /inference="COORDINATES: protein motif:HMM:NF019323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HMZ63998.1"
                     /translation="MFGRLRFYLRFMARFKSSNAANQNNVSMSNDKKLGSFDKPKRGK
                     FKFAPHVSKLFTGFFGHSFSRGSKRSNQQNLFRNILVGSNSNPMSVYWSLIHSKLCAQ
                     RLILSKILLVLLTYSIYPQENKLNLEENFTSKNYYDGYVLDTKTKLGIENATVEMKNQ
                     NLGVGYYSVKTDKKGYFQIKDFIPEINYQITIQADGFVNFSASNLLFVTKDKPKYFLQ
                     KEGILYGVVKDSRGKNLEGVEVKIQSENYSLPRRSNTVITNAKGFYKFEKLSEGPYSV
                     SFEKSGRIGETAKIKKLKSGEKFHLPMRLFNNASVSGTVLIDGLNVPAIGMNVVLSSL
                     RATHSASTFSNGEFRIEDIKPGTYKLKLSHRGFHEIEKENIVITEGKDFEKLSFIAKP
                     KEPEITVSTNRYVFSPADKIQFDLRTFRIEKVNIKIYKVPEKEQAEMLLLQDANKLNP
                     VDRKFQLITEWEENMRNFQLYDWNYMQLNLKDKLPTATYLISVVGEDKKYYANKFFSV
                     TSVGIVVKRSLDSVFVYASDLISNLPMGGAKFYFYEEIPPVNEKKKTEEGAVSTESES
                     YSSEGGTEDYYTYEVDKPSSITGYNFKLIYEGLADESGTYQYKAKNEKNILVVASGLD
                     GSNAFSETGKPKSYLSEKNKFFIYTERPVYRVGDSIFFKIIGKKSDGKFLPDARVKLN
                     YKITSPDGETIETKDIELDDWGTYSGEFISKNQDALGEYGINVSKDKSELSAGSAYFY
                     IEQYRKPEYKVEVTPAENAYVNGETAEFKVEAKYFFGAPLANANMSFRIYENKLRDTN
                     TTYWWEEEYEANTTYNQIRMEGDKELNENGLAVLKYKLGNYPYDREITLEATVTDKSN
                     VSITSRKTITVGRGKFYIKINPVNSFFYTKDKKEFEIKTLDYEGKPVSANVTLQFFPF
                     IWKPWQRVYMHADKPVFIQKISTDEKGIYKLELNNELATAGEFDIVATSQDSMQNQIT
                     ASRVVWLYSGTNDRLESKFKNIELTLDKNAIDKPGEITVLLKSKFVDNFVCVTLEGRD
                     IYDRKVVKMEGNLQAVKLNIKPEYAPNLFITATMQKNRALYTTSQSLEFPHVDTRVMI
                     KVKTDKEKYLPRETIKVEITAQNEEGKPIEGDFSLGAVDEAIYQIRPDHTRAMNPFFY
                     SKISNWVSTNYSYPMTLLAGTTKDGKDLIREVFKDTAFWKPDLRTDKNGKAFIQFEVP
                     DNLTEWRLTLRGHDKEGRVGETVSSVLVTQDLVARIAKPRFFTESDRISVIGIVNNNS
                     KEGMPEIKTEMLVNEKEIKPEEKDKISLPPFGASKQHYPLVVPETDSMKLQFRASANE
                     KAKDAILHKVPIYKNGIAFKISEQGDMTENTVVQLRPAKPDKNISAHAEELSISLNPS
                     PVEKICAARDFLADYPYGCVEQTLNRFLPAVEIWNLNQAKKLPSIKDDPNLEKKVIEG
                     IKRLSEMQNDDGSWGWWSGDVGNEFLTAYVMYGMKRVSVLHIDQNAVQKGKTALTRML
                     SNPQSMEDDARAYLLFVGSLYDIWSPSSYEKLIKKDDVSAYAKSFLIRALVNLSPKAF
                     TKDGEKIVSELKKQIPILVERLKSSHKNDKRGIYYDHSEETYYSWQGGSTEVSAHVLS
                     ALLEADDKSAITANLMSSIQKRGSGGYWNSTKETATVISAITSYLDKKGANFSSKGKV
                     DFSLNGKPLTSIEYDTSKSDRELKKMIPIKESKDEYTIAATGTKSSDLSFDLSLKGFL
                     KFKDNDTNSQATQTSIDSLRNGLKVKRGFYSIKRIRDLNNQEYFVPHPLVQRKLQVGE
                     ELLVKVQFKADKDYEYLMLEDFLPSGFEVTKTDAYEGRVYYTHSERRDEKMVFFFNKL
                     KKGKVYELAYMIRAELPGVFNVKPARMECMYAPEIQGFSLPEKLTVEK"
     gene            complement(48885..49646)
                     /locus_tag="PLF66_07895"
     CDS             complement(48885..49646)
                     /locus_tag="PLF66_07895"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004441842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HMZ63999.1"
                     /translation="MTLPISVCIITLNEEDNIGRCLASLDFADEIIIVDSGSTDNTTV
                     IARDYPKTSLYYRKFDTYINQKNYCKSLAKNQWVLALDADEEISLDLKKEILSVSKEK
                     MNEVVGFFIPRMTLYMGKWIRHGGWYPNYQMRFFQKDKGEFSGILVHETVSIQGKVEY
                     FKNPLSHYSYRNISDHLKFIDRYSELAAVEKFKKGKKSGLSLAIAEAIWKFISMYIIR
                     FGFLDGKVGLIIAILGSYYNFLKYIKLYELNQKKT"
     gene            complement(49657..50331)
                     /locus_tag="PLF66_07900"
     CDS             complement(49657..50331)
                     /locus_tag="PLF66_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004459014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HMZ64000.1"
                     /translation="MQINQLDSQYDMEFIQQNANEIQIKTYRTSRHLEERVQETLQGI
                     LTKFEKERLIPILYTVIKELIINATKANQKRVFFEENHFDIKNPEHYAIGIKEYKKIF
                     SENMGQAYAPKCKAKDYYCLIIFDYDASGLKIEIRNNTLIAEQEEKSIREKLATAMSY
                     DDLAQFYMDNADNTEGAGLGLALIIIMMKGEGIDPNYFRISITNEYTSARLEIPFTNE
                     YKSARN"
BASE COUNT        16781 a         8007 c         9202 g        16429 t
ORIGIN      
        1 caaaagaatt gggaaagatg actttaaaca ttgatgttgt gcaagagaga gtagaaggaa
       61 gaattttggt tgattctgaa atggttaaaa atcgattgct aggcgatgtt tctcaattaa
      121 aagcagaact aaaagcgaac ggattggaat tgcaaaatat ttcgattgaa gttagaaatg
      181 aaagcacaca agcttttgaa ttcgcaaatg cttctagtga caaagattcc tcgagagaga
      241 atggatctag ttcgtctaac ggatctttat cacagaatca aacagaagaa atggaagttg
      301 aactaggata cactagacgc tctaataatc ttgtagatat aaaggtttga tataaactat
      361 gccaacacca ccgatatcag gtattaaacc aaaatcaata gatccttcta cggaatctac
      421 taaaacaaag tatttagatg ctgataaaaa agtagatatc aaatcttatt tggataaatt
      481 atctaaagaa gaaaaatcag ggctcaaagg aattgaagtt agggaaactc ctaagcaatt
      541 gggaaaagat gattttttga aattattgat tactcaatta tcccaacaag atcctaccaa
      601 tccaatgaaa gatcaggatt ttattgctca aatggcgcaa ttctctagct tagagcaaat
      661 gaaaaatatt tcagctggta ttactcgaat ggaggcaaaa caaagttatt cgctcgttgg
      721 aaaagtcgta tccggtcctg atatggtgac tggtgagacg gtagttggaa tagctgctgc
      781 attattcttt gatgcagatg gaaagtctta tgtaagagta aatggtcgtt ctgttgaggt
      841 agaaaaaatt accctcatct ccgatccgtc tgttttaaac caagaaaaag aaatttctac
      901 aaataatata aaagaaaatc aaatcgaaaa aaaaactagt attcaagaaa atcagaatct
      961 ctccgatatt ggtacagcaa aacaatcagt acacgaaacg atccaatctt tggataagcc
     1021 agtggactgg aactatcccg gagctaaaac aaataaaggc acaaattatg agaaataaaa
     1081 aaaataaccc agaggtaatt gcaatatgat gcgatctttg tattccggtg tttccggatt
     1141 aaaaaatcat caggttcgta tggatgtaat tggaaataat atttccaatg tgaacacaca
     1201 cggttttaaa acagagcgtg ttacatttca agatatgatt tcacaggagc tttcggctgc
     1261 tatggaacca aaggacaata ttgggggagt aaacccaaaa caagttggtc taggttcttt
     1321 gatcgctgca atagacaaaa tcatgactca gggatcgttc caaacaactg gaaagaatac
     1381 tgacattgca atttctggtg aaggtttttt tgtcgttaaa gatggggata aagaatttta
     1441 tactcgtgcg ggagcattta acgtagataa aaacggatac tatgtaaacc ctgcaaatgg
     1501 tctcaaagta caaggttgga atgctagagt ggatgaaaac ggaaataagt acgtaaatac
     1561 tgcttctagt gtagaagaca ttgttttacc tctctattct aaagagccag caaaggctac
     1621 aaaagaagtt atttttcaat ccaatttaaa tgcctctgtt ttagcagtgg atgaaggatc
     1681 aaagccagaa gatattaagc gttatatcaa cgatccagat ccaaagaaaa gaagaggaca
     1741 tcaaaccagt atcatcgttt acgatgacca aggcattgaa cgtgaaatta aaatggaaat
     1801 gtataaaatc agagacaatg tttggagagc ttccgtttct atgacagatg caactcaagt
     1861 ttcgattgat gtagcaacta aagatcaaaa cacaacagtt cctggaaaca cagaaatcga
     1921 actcagtttt tcaccggatg gaagacttgt aggtgtatca gatggagtag atagcttaag
     1981 ttcaggtaaa ttaaatgcag aagtttcttt tagaatcgca ggaaatcctg aaaagcaatc
     2041 cttttcttta aatctaggtg aatcaggaat ggttactgga ataacacaat ttgcttctga
     2101 tttcacaaca aaggcaatca aacaagatgg ttatccaatg ggatatatgg agtccttttc
     2161 gatcgacaat tctggtagaa ttgtaggagt ttattcgaat ggaattcaac aaccacttgc
     2221 acaagtggca atggcactct ttacgaaccc agctggttta aataaggcgg gagacaccat
     2281 gtattcgtac tcaattaact ctggagaacc aaatattggt gagtctggga ttgctggtcg
     2341 cggtaaagta aatgctggga tattagagat gtcaaacgta gacctctctg atcaatttac
     2401 agatatgatc gttacccaaa gagggtttca ggcaaattct agaacaatca ctacatcgga
     2461 tcaaatgatc caagaagtct taggtctcaa gcgataattt ttaagagacg attgagatag
     2521 agatacaagc cactggctaa ctcaggttag ttagtggctt tttaattttc tactctaaaa
     2581 attaaaaaag tatttgtaat ttaaacattt atggtaaaac taaattgtcc tttgaatttt
     2641 agaaccaaat gacaaagatt caatattaga aagaaggaaa atttgcagtt aacgcagttt
     2701 gtaatttcag acttaccaga tttggaatcg aaagtttcct ctttcgctaa gcaaatcaat
     2761 gcagtctatt ttcagttatc tcaggcgcat tcttttcctg aacattcgga aagatttata
     2821 aaagtcgtat tctatctaaa gaaagagact ctacaaaatt cccaaaaaga aattagaagt
     2881 ttattaaaat acaatcctaa tatattggct ctcttagttg taaatgctcc tcccgaagaa
     2941 aatatttttg aaacgaccga cttggagaat gatttatttt tttctaatat tcaggattct
     3001 gttccggctg tttttttatc caagactctt gtaaatgcat ttagtttctt acagctccga
     3061 ttagaaagtt ttgagttaca atacaaagta aatttaagca ctagtcatat ttctaaacta
     3121 actcgaatag gcgttagtct ctcaaatgaa aaggatttta ctaaactctt aagagatatt
     3181 ctatacagtg ctcgagagat ttccgtggcg gattctggat ctctctattt aatagataaa
     3241 gatgaaaaag gaaatcctaa aaatcttcga tttaagattt ctgccatgga cttaaatagt
     3301 agtgaattta ttttacccat aaataagcaa agtatcgcag gctatgttgc attcaccgga
     3361 aaagttttaa atcttccaga tgtatacaat ttaccaaaag atgtagaata cagttttaat
     3421 agagattttg ataaagcaaa gaactaccat tctaaaagta tgctagttgt ccccatgaaa
     3481 aattatttag gggaagtgat cggagtcatt caattaatca atcgcaagaa aaattttaat
     3541 agtaaactat cagtcgaaca aatgaggggc aatgacgtta tgccctttga taagtattcg
     3601 gaagaacttg tgatgagtgt ggcaggacag gctgctgtcg ccattcaaaa caataattta
     3661 attcaggata ttgaagctct ttttgaaggg tttgtgactg cttctgtaag tgcaattgaa
     3721 gcccgagatc ctaccaccag tggtcattcc tttcgtgttg ctttacttac agtcggtctt
     3781 gccgaaacag ttgatagaat ttctgtcggt aagttttcta atataaaatt tacaaatgag
     3841 caattaaaag aaatacgtta tgcctcactt ctacatgatt ttggtaaagt aggggtaaga
     3901 gagaaagttt tagtcaaatc taaaaaatta gaaccctatg aattagattt aatcaaatgg
     3961 cgttttcatt atatggttaa ggatttacag gaacgcttta ctgctcgaaa attagattat
     4021 gttcgtaaga atggaaatac tggtttcctt gaatttgaaa aagctcttga gatggaatat
     4081 gcaatcgaga agaaaaagtt agatgatatg ttgcaagtta ttattacttc gaatgaacct
     4141 acaattctag aacaatctaa ttctcaggtc ttagaagata tttccaaaat gaatttttct
     4201 tttttggatg gttctgagcg acctgttcta acatctgcgg aattgaatta tttatcaatc
     4261 agaaaaggct ctctcgattt cgaagaaaga aaagaaatag aatctcacgt tgaacatacc
     4321 tttcaatttt taagtaaaat tccttggact tccggtctta agatggttcc tagtattgcg
     4381 catgctcacc atgaaaagtt aaatggagaa ggctatccaa ggggtttaat tgcaaatgat
     4441 attccgatcc aatcgaaaat gatgactatc gctgatattt ttgatgcgct tacggataaa
     4501 gatagaccct ataaaaaagc agttccactc gagcgtgctt tagatatttt gaaaatggaa
     4561 gtaaaagatc accatgtaga tggagattta ttaaatattt ttatcgaatc gagggttttc
     4621 gaaagactaa atactaacaa acaaatttct taaggatagt acatgtcaaa tcaacctcta
     4681 caaaactgta atatactcat tacaggtatt actgatttag gttcccttgc tctagcagca
     4741 gcaagacaag taaaaaaaga aggtgcaaat ctgatttgca ctgggctagg tttaacacct
     4801 tatcattcca atttatctga taaagcaaaa gcttatttag acaaaacata ttctgatttt
     4861 aaagaaacaa tcgcaaaaga gtttggtgat gatactctta ctctgccgtt agatgttaca
     4921 ttagatggta gcctagaaga tgtggcaaat acactcaaag caaaggggat caaattaaat
     4981 ggtctgcttc attccattgc gatggataag accattcgtg ctggggctgt aaaaccactg
     5041 atcgaagttt caagagaaga gtttatggga gcaatggatg tttctgctta ttcgctgatt
     5101 gctttaacta gaagcttact ccaaaaagaa gttctacaaa aaaactcatc tattttatct
     5161 atcagttatc taggtgctga aaagattgtg cgtcaccctt acaaaaatgt tggcgttgca
     5221 aaagcagcac tcgagagaat cacaagagag cttgctttcg agttaggtaa gtctcatcaa
     5281 atcaaagtaa attccattcg tttctctcca tttactggaa gcaaagcagg gggagcgatt
     5341 caaggacttc aggaagcgtt tgattttgct gaagaaaatg caccgcttgg aaatgcaatt
     5401 ccagaagacc ttggttatga agtcgcttat ctactcagac cttatggaag aatcactggc
     5461 gagattcgtc acgtagacgg cggatacaat atacgaggat aatagacgtc agttctatag
     5521 cagaaaatta aaagattacc accgataaac accgaggaca ccgataaaag atagaaaatt
     5581 taagaccatc ggtgttcatc tgtgtttatc ggtggtgaaa aattcttgta atgagtttgt
     5641 tttggtcact gtctgtattg gttggctcga atatttttag tggctaacgt agcagtttct
     5701 tcgattctat ttgctcttgt ttcttctttt tttgcattta gaatccactc tagaatgcta
     5761 cgttttacag atctaggaaa agcttgaaaa ttttcaaaag ctaacttttt acttttcaaa
     5821 gctttttcta aatccttcgg aatagtaaga ttttctactt cgtctaacgc attccaagtt
     5881 ccctttttct ttgcaagctc aatagaatta aaacctgctt cagtcatgag tccttctttt
     5941 ataagtcttt ctactttttc tttatttacc ttactccaat tactcttggg attccgttta
     6001 gagaagaatt ggtaaaaact aatctcatcc tttttattta ttttgctatc aatccatcca
     6061 taacagaggg cttcattaac agcttcatca tagtatacgg atttaactcc actttctttt
     6121 ttgtagataa tgagccagat gttttcttct ttcttatggt ttttttctaa ccattgcctc
     6181 cattcttttt tagatttagc gtgaaaacta ttttccttcc cttctttatt ctttgtgggc
     6241 ataggtattt ctcatatatt taggaagtag ggtcaaatag gaattatccg caagagtaag
     6301 accttctagt ttagaaatat ttctttttaa aatggaaatt ggatttggca gtagggattc
     6361 gattgtaaaa tcgtgtttta ctaaatttgt atctgctatg attattctat tttctgtgtc
     6421 tagttcttct ttgaattggt tttcgaattc actaggagat atgtcaaagg ttcctttgta
     6481 agtttcgttt cggaaagagc ctgcaaaaac tttcttttgt tttccatcta agactgcaat
     6541 tacgggagat ttataaaaat cagaatagaa attagaatac atttcaattg tatcaaatcc
     6601 gagatttgga attttccaga gctggcttaa attccgagct gtagcgactg aaattcgaat
     6661 tcctgtaaat gaccctggtc caatcgcaca gcttacaaca tctggtttat cccagctaac
     6721 gcttgttagc cccaactgaa tatcagaaag tagtctataa gaagattctc ttgggtggtt
     6781 gcctgcataa aaatattctt cttttatttc cttgttttgt aaggaataaa gaccaactaa
     6841 aataaaatca caacttgtat caaaaaataa aatccgcaca gattcattct ggatttttat
     6901 atctctaggt ctattcaaaa aaagggattt aagtaaggga tttccaccga ggagaccgat
     6961 aactgaacga tacagaaacg atggataatt ttgaattatc gtatctttta tcggtgcgat
     7021 gcggtgagcc tagtcgaacc gttcatcgtc tttccaccca aaatccaggg attgtgtaaa
     7081 aacaagagtt tcaccacgaa gggcacgaag gatcacgaag agtagcgact ctcaatgaag
     7141 aagagtttac gttaaggcgc aacgtcgcga agtgtctttg tttgtataaa tctatgtgct
     7201 taaataaagt ctgtttttag ataagaattt taatagattc tataagtctt aattgttaag
     7261 gttgtaatag aaaacactta gaagagtcta gtggaattcg aaaaaaagga ttctgatatt
     7321 tatacattta tctaaatcag gattaaaatt ttaaattcaa ctcagggaca aaatgcgagt
     7381 tcataaattc gttctgcttt ttattttatt gttttagctt agttgaatgc caaaagcggg
     7441 aaggaaaaat taatgctacg gctttttggt attatgcggc tttgatttat ctttctattg
     7501 aggaaataaa aaggcaatgc tctcagcaaa atgctttacc atcatatatg ccacaatcgc
     7561 cccttgtttc tgaaggttgt attaataata gagtttcaag tggcttaata tttgatgttg
     7621 atgtaaatca aaatatttcg gttgatactc taacgtccgg aaagagattc aaatgtttat
     7681 gcaatggaaa tataggtaaa tatggttatt catggaaaag aagcaaagaa ttgtttgatt
     7741 cgatgccgta gcctcctcct gatttagata taattttttt ttattcaatc tcctcttcta
     7801 tttgaaaaga tgagatttta acgaatgcaa ggtataaagc caatgataat ttattttgta
     7861 tcacaacctg caatattaat ttgcatactt accagatcca atatcaattt ttaaaaataa
     7921 gataaggttc tagatgttta ttgtagtggc ttatattaaa aaaatgaaaa cacttcactt
     7981 aaataaaaag tgaaatgttt tctttggtag gatactctat taaaacccgc ttgcagtgat
     8041 agcagatcta gagcatggat tgttgtttgg atcacaggta tacattttaa atataaaatt
     8101 ttgatctgga tctggacgtt gtgaggaact tcctaggttt gtatttgttg ttccggtaaa
     8161 gcccaaggaa gtgcagttag aagtagagag tgcatatttt ttataatcat tgtttaataa
     8221 gcctccgcct ccacctgctg gtgtattgta ataattggat ggactcacag gaattccttt
     8281 tagggtaaat gtatcaaagc attgacctgt aaaagaaaga ttcgatagat ctgctctttg
     8341 catgatgact aacgatccat ttgtataacg atctgtggca gttccagatg cccaatttct
     8401 taagacgagt gcggttaaac cgcttctatt ttgttttttt gtttgaatac aaccaatcga
     8461 tagagtaaag attgtaagtg ctaataaaat tctatttaga ttcttgttca ttttccttct
     8521 cctagtttgg tctgtagcta tatgcttcag gggaaacagt tcctgtccag aagttacctt
     8581 gtaattgaaa atagattctt ctatcatcaa ttgcagcata atttccagta tttggattca
     8641 catcaaattt atttcctaat acttgatagt agagttgtgc tttgaaatga tgtttgtcac
     8701 caaacaagtt taaacctgcc caataaactc ttaagatatc attttggttg tggctatttc
     8761 catctctatg aaagtcaccc atgatttgtt catattttaa aactggcatt atataatatt
     8821 tatcccagaa tttaaggttg tatccaaccg ttgtgtgcca tgcatgcaga ttattggaag
     8881 cagcacctgt aaatttggta aaggctcctg ttagataaat tccttggtat gtaaaagtag
     8941 tatcatacgt gtgacccaca agaccgaagc ttggacgacc cggagttgta acgttgttct
     9001 gaacattgta atttggaagt gtcattgcga tattatttac agttgagctg aatgttgttc
     9061 cacgatcagg gttggattgt tgtccattga gtaggttaaa tgcatttgtc gttccaggtg
     9121 tatactcagg aacggcaagt gtgtttggtt ggaaatttgc tgtttgcata acagctgccc
     9181 caattgacca tttcatatca ctttggaaaa cttcttctcc ttcttgccag tttacttctt
     9241 ttccatccac tgctcttttc agtccgccaa agacattgta ttggatacga ccataataag
     9301 taggtgaaat caaagtagtg ccccaacgat tagaagtagt aaggtcttgt cgtctaccgg
     9361 ttccataatc cccaccagca cctttaccat ttccaatcat gaaagatact tgtaagtatc
     9421 tttcccattt atgacctaat tcttttaaag gatttgctga aagcataact cccaaatcaa
     9481 attgtggtag tgcatttgtt atgatacttc gttctaatgt aatgaagttt gcagaagatt
     9541 gtaaatactc tcttgtgaat tgagtattga tttgtccaaa tgtcaagcga agtccagcat
     9601 aagggtgttt tgcataaaca aaagcttcat gcacataacc tctattatct gtcatacgca
     9661 cagtgttaac aactggagtt gtcgtggttg tgatgactcc cccattatta gaaactcccg
     9721 ttacaacgtt agttgttgta gtaactgtat ttggtctatt taccatattt tccatacgga
     9781 gttggatatt cattccccac caatcgtttt catacatagc accgagtcgg agtcgtctaa
     9841 aattccaatc tagagcgcta aaatcattat taccattgct atacggtgat tgttcactac
     9901 ctgttactcc tctaaattga attcttccgt aaactgttag tttttcttta gaggtatcat
     9961 cgggacgatg tgcgaaagca tctgggagtg tagtagagag tttttgtggt tctacttttg
    10021 ttctgcctgg acctggtttt gtaaagagtt gtcctgtttc attgtcttca taaacttctt
    10081 ttagaccttt tgacgctggc ttcggagcat gatcttttgc atgatcagtt tctgttgctg
    10141 tattgggctc tgttgttttc gtttcatttt gtgcataaat tgtaaaactg aaaagcaata
    10201 agactcctag tattaaattc ttctgtttca tagttttcct tgtttatagt gattttttat
    10261 caaaagttca ttttttctga attattagga gtaactcagg attgaccatt tgttttctat
    10321 atgtttcaaa attaaaacat tcgcatttct aatgaattac tagaatatta caaatttatt
    10381 acaatgcaaa cctattgaaa aatagaataa ttactattgc aataaaatgc gttagtaggg
    10441 ataaggaatt gatggtttag ttggttgtaa ttagagagga atttgtaagt ttgttgtcaa
    10501 tcatggtgta gaaaaaaact tttacgcaca cttcgttgag atcctaggaa ggaatatagg
    10561 taggtaagaa gtaagattct attgcaatta aaatctaagg ctgtcttttg gagactagcc
    10621 ttagaagaag ggagttataa tttctcttta acgattggat gaaagaactc aggaagagtt
    10681 ttgatttttt ccttgagtgt ttctttacga atgccattcc attctggatg ggcagctgag
    10741 agagaatcta tgtttttaaa aagattttct ataaaatctg aaatttcttt ttgcgataaa
    10801 tcctttctag caagtaaaac tgtttgcact tgtagagtag acactgcttc tttttgccag
    10861 ctgtatgtga gggctggaat ggttgatttt tgataaactt tattgtcatc tttgatttgt
    10921 cctgtgacag attcatctaa acttaagatc tttagtttag aagctgcatc ctcttttaaa
    10981 gcggaaagaa tttttacagg aagaccagaa acgataaaga gtccatcgat ttcatttttt
    11041 aagagcaaag gaagtgcttc ctccgggccg atttctttta cgtctacttc actaaaatca
    11101 attccactga ttcgtaaaac agaaatcgaa gttgccttga ttccagattc agagtgaccg
    11161 attgcaattt ttttcccttt taggtctttg attgttttga tagaaggatt cacaatcaaa
    11221 tgaatttcat cctgaagaac gggaagtaaa acgttaattc ctttttgaat cgccggattt
    11281 cctaatttcg cactgtaaaa catgtcgatc tgtgttaaac agaaatctgc ttttttatct
    11341 aaaagtagat tgatattatc tagacttccg tttgacggaa tgacttttac atttagagat
    11401 tgatttgctt ctagagactt tgccattgta tcataggttc caccttggaa tccgcctgca
    11461 aaaagagttt gctctttttt cgatttacaa tggagcatga cgagggtaat cagtgtaagc
    11521 cccaatattt tttttatcat ataactatcc ttgaatgaat gtttgaaacc atatttagta
    11581 aaaggtttac ttttacaatt aaaacaaatt gtaaaaccga ctgaaatgta atagaaatgt
    11641 aaaggaattg aaaaattaaa tttaaatagg cataaaaaaa gactgccttt tgaagggcag
    11701 tctttaaaag agataagatt cttatactta gtttcgtgtt atcgctacgg gagatatatt
    11761 tgttacgata aaagaaccag atacgtctcc atctactctt gttaatttta cataggtata
    11821 ataggtgtag ccttccgaga gtcgatagac gcgatagttt cctgccttgt ctactacttc
    11881 cacccaacca actttccaag ttccaccttc ttcgttgctc gcagttggaa aggttccagt
    11941 tgcttcatat tttagagtcg aagtattgta ggtggtgctt agagtctttg tggttccgag
    12001 tgtattatca agtagtttca aaggactata caatacaata ttgaccgctt tcacaccaga
    12061 cgcattggtg ctactgttat ttgttgttaa agtagttgag cttgttgttg cattttgatc
    12121 tgcagatgtt gtttctgtag ttccatctgc tacatcagca ctgattgtaa agctttggtc
    12181 aaagtttaca tctgtcttgt taatggagat agattttaac tcaggaggat aaaagtctgc
    12241 agttgcagtt gttgatgctc caatgattgt aaaacttttg tttaaagttg tgcgagaagt
    12301 ccaaagcgag tttcctgctt tgtctgtaac ccagattcca cctaagaacc actgcccatc
    12361 aggagcagta tttccttctg caaactgacg aatggttgca gagccagaat aagttccatt
    12421 tgtgttcgtt ccagatgtta gatctagtct tatgtagatt gcatttccta agtagttgct
    12481 attcgctgac caccaagaat aactattcaa acgagcatcc atataagcga ttccactcgc
    12541 atttcccgtt ccacctgtat cagttgctcc tgttactgta acagtgatca caacttcttt
    12601 tggactagcg ctagaaactg aaatatctac gtttgatgca attgggtttt gaactgttgc
    12661 acccgaattg atatctgatt ttagagtgac agttcctaga gttggagctg ctgtatcagg
    12721 gcttgagcct ttgactgtga aagtaggaat tgtgatttta ctatcgatct tttgaccaca
    12781 accagtaaag gtataattcg ttgtgctctt gctactatcg tatagatatt gtctttcatt
    12841 ccctgtgacg tctcttgctg ttattaaacc tacattccaa gttccattgt ctacaaaatt
    12901 agtaagagtt gcatctcctt catagaaacc tgtgtttgca tttagagtaa gagttgcatg
    12961 agctgtgtat ccacttcctg taattctttt aggactaaat aattttgcag taacggactt
    13021 cactcctgtt gctcttttgt cactagaagt tccttcgtat ccatctgata cttttgctga
    13081 aatacgaagc ttaccagatc cagaagatac atctactgta gaactatcaa tgctcacaga
    13141 agtgagttca ggtgccgcag tatcgccttt tggattgtaa ccgcatacaa aagaaatcgg
    13201 tgcatttgga attgcattta aaatatttcc ggatactctg atgataacta ctttgataga
    13261 gccatctgag taagtgatcg ttgccttccc atctttattg agtccaagag aagaaccact
    13321 ttcaatttta attttaaatg ccataccttt gttgtctgtc gttgtaacaa ctgcttggta
    13381 ttctcctact gctaatgtaa atacaacgtt cccatttgta tcggtttgtg cagaagtagt
    13441 aaacactgga cctgttgttg tgctggttgt gctattcgtg ctcgttgtag tagtggaaga
    13501 agcttccctc acgctagatg aagtggtggt tgttgcggga gaaaaataaa ccatagcatt
    13561 tgaggttgca gatgttgtgg atatattggc actgacttgg taggtagcag gttgagccaa
    13621 taaaagaaga gttgcctcgt tggttgaact tttctttaca tcaatacaaa aggaaagtgc
    13681 taagagagag aaagatgtga gtagaaattt tttcatatga tttcctcaaa attttaagtt
    13741 gtataaaaga taactagacc ctttatattt ataacaaacc agttttccaa atttttcagg
    13801 gataaacctg tttttgctac agagaaatct agaacctgat atttggattt gtcaaccaat
    13861 ttttgaatcg gttctcaaga aaagatttta gttcaatttg gatttagaaa ataaggtttc
    13921 atctacatcg caaaaagcaa tcaatctgtt ataaaataaa atggaaccgc gaataaatgc
    13981 cggtcttatc gtcgtaaatc tttggaattt aatcttgaat acgaaagaaa agtaacgtta
    14041 ggtattaatt aaaaaatgaa gacaaataat aaaattttag gaagagctta aaaaagattt
    14101 taggagcaaa ggatgattcc tagaaaggtt ggatgtctct attcatacat aagatagatt
    14161 tctaaataca gaattccgaa tggacctcag taaaaataaa ttgaatgatt tagaaatctt
    14221 tggattctat cctagatggt tcattttgaa aagactcaga agaattcaac accttggcgt
    14281 attgtagtag cagatgatca ttctgtcgct cattttggaa ttgaggctgt tcttgcggct
    14341 aatcctgagt ttactatcgt ttctaaaatt tcagatggga ggcaaataaa aattaccctt
    14401 caacaagaag aggctgatat tttaatatta gatgtcgatt tgcctgaact atcaggaatt
    14461 gattttttaa gagaagaaag aagtaatttc ccaaatttga aaatactatt atttactgtt
    14521 catgaggggg agggcttctt tcgagaagct cttaggcttg gggctaatgg ttatttttta
    14581 aagtcagatc atattttaaa tatgccaaag gcgctttcct gcttagtaaa aaatgaattt
    14641 tattgttctg aaaaaataaa atcctatctt ttaaaaacag aaaaatcgga tgaattgagt
    14701 agtaaagaaa tcgaaatatt aaaaatggta gtagcgggtt tctcggctcg tgaaattgga
    14761 gaaagaatgg aaatttcgag acgaaccatt gaatactata ttacccgcct aaaagaaaaa
    14821 ttagatgcag aaaatatagt agaacttgta gtcactgcca aagaaaaata ttttttataa
    14881 aatgaaaata gttcaagttt taattctgct tctatttagt tattctagtc aatatgcctt
    14941 tgatcgagtg gacttaccaa ctaaagggtc tagtcattta acagaaaaaa tccattattt
    15001 tgaatctgat tcagaggatc taattcctga aataataaaa gagagtaaat ctttcccttg
    15061 gcagaaaagc aatctttcca ttttaaactt tggatttaca aaaaaaaata tttatatagc
    15121 gctaaacgtt cgtattctag ggaatgaaaa ccaatatttg gtgattaata ctcctaggat
    15181 tgatcgaatt cttttttatt ccccagccaa aggagtttat cgagaaagta gggcagggat
    15241 actcgatcct ttatccgtaa gagagttttc tcatcgaaat ccaatttttt tattggagaa
    15301 aacacaagaa gaggagacta tcgtattttt tcatatcaaa tcagagacat ctctccaatt
    15361 tgaattaatt tcctatgatc ataaaagtct attagaaaca actgcaagtg aacaaattta
    15421 ttttggtatt tttattgggg ttgcgattct atctgtttta tatggtctta tgctctttca
    15481 tttgatgaaa gattttgcga atctgtattt ctctatttat agtttattta gtttgattac
    15541 tattttgatt tatacggaat ttggttttcc atatctatgg gcgaattatc catctttcaa
    15601 tcattttaat ttattttcaa ttttgctatc tgccattttt ggagtcttgt tttataggaa
    15661 tgttttaaat attgataaaa atagtcctgt tttaaatcgg atttttaatt ttaagctgat
    15721 tgtattgcta ataacgtttc ctcttctgtt ttttataaag ttctctcttt ctttaaagat
    15781 agttttattt gaaattgttc tagtcatgcc aattatatta gtttctggat ttaaaatttc
    15841 ccttttgaga tataagcctg gggtttatgt atttctaagt tctacattat ggtatattga
    15901 atttctagtt cgagttcttg aatacaatca gttcttagct tttgatttta gttttttaga
    15961 tacactttta tttcgtattc tagaaagagt aattctttgt attgcgctca tggagagagt
    16021 gagagcaatt aaaaatgaaa aggagcaaat ccaaaaaagc aagttagaat atgaaatcag
    16081 cgttcaaaaa gagctgagtc ttctcaatca aaatatggaa tctctcgtaa aagaaagaac
    16141 cttggaattg gaaaaagaga aagaagcggc tttacatgca aatttactta tgaatagttt
    16201 tgtcactact gtttcgcatg atttaaagtc tcctatttta aatattcgta atttattaga
    16261 tatcatgatt gtaaaagaag aaatggattg ggaagagaga atttattatt taaaaaattg
    16321 tagaaatgct cttacatctt cttatcaact agttaataac ttactcaatg tggagagatt
    16381 tgagtctgga aatttagaaa ttacctatca atatttagat atcaacacaa tcttaaaaga
    16441 aacaatccaa gagcttgcct ttattttaaa tgcaaagaat ataaaaatcg aattccaaga
    16501 aaaagaagat atgattatca ttggagatag acaattagtc ttgcagctct tcttaaatat
    16561 tctttcaaat gcaataaaat tttctcatga aaatggtaag attcaaatcc tttctcagtt
    16621 caatcaaaac tattacgaga taattatttc agatgaagga attgggatga gtcaagcaga
    16681 tgtaagtagg cttcaaggta attcgatcta cagtgaaaaa ggaacaaaag gagaggtagg
    16741 ttctggattt ggatttggaa tcataaaaaa gatcgttagt ctccatgatg gtaaaattca
    16801 attgaaatca aaaaaaaaca ttggaacaga gattcaaatt tctcttcctt gtcaggacaa
    16861 agttgcgatt tgtatttggg atctaccgct tgattattct attttaagag agaaggcaga
    16921 atgtaattcg attcgactct tgagaatatt tgatttaaat aaagtaacta agcttttaga
    16981 aacaatttct ccagactttt ttgtaattgg tctttctttt cgtggatttg aatctatatc
    17041 caaagaactt ttgaatttac tgaaattttt agaaaataag tcgatgcagg tttatctcgt
    17101 cagttcgaga gatgtatttg aaaaaatcaa agagcagaaa aatctactct ttgttataag
    17161 tgaaaataaa aatttagaaa acatatttgt ttaattagag acggttcctt tgttcccata
    17221 aaaataggaa cccattaaag tataaatggt agcagtgctg tcataggcat aatgataatg
    17281 atatgtagca gtgctaaaat gagttgtctt tgctgtatgg ccatgtaaag aatctaagtc
    17341 tgttggagta aaatcatcag acgttgagct agttgcatta tcgcattttt gcccatagat
    17401 tgcataacca tctaatgaaa ttcctatgag agtagagtta ttacaagaag cactgttact
    17461 gactaacgtt gtattatcac aaactttagg aacagccgca tgatggtgat acattccagt
    17521 tccttctggg tgacctgaga aagaatcaaa tgacacagct tcttttgcaa gtgtatctgg
    17581 tggagcagca gcattattat aaattgcaag tccattgact gtcattccaa tggaaactaa
    17641 accaccttgt gtgctatctg ttcctgttcc tttcgttgga gtggatggaa tggtgtaaac
    17701 tagcttttga cttgcaatgg tgcttggatt cccagccgta gcagaatcat acaaaggaga
    17761 agtagaacca aaataatagg atttgtggtt tggtatgttt tgtgatttga aaacgtattt
    17821 accatttgaa tcgatatagc caacagcgca tttaaagtta tttttgatcc aagaaggaag
    17881 agaactatcc atacaagttg caactccgct tacacaccca gtcgagttat cgatagtcgc
    17941 ggtgctattt gtgacggagg ttccagttga tgtggaggaa gaacagcttg tagatccagt
    18001 tgttctggtt gctagatagg ctgcggatgc gtattgtgct gatttattat tatcattatt
    18061 tccacctact agattgttgc aggtttgtaa aaatggaatt agtaagctaa ttcctatcag
    18121 tgtatatttc atgaaatact ccttataccg aatatgattt tgagaaaatt tttcatattg
    18181 gattccttac tataagagga tagaagcagt aatacaattc attttgtaaa aaggaagtgc
    18241 ttggacgttt agtactttta cgaacttttg tttccaatct agaatctagg gagtatatct
    18301 gagtagattc ctcgattcaa atgggtaaaa cacaaactct ccgcagatga aaccttagga
    18361 ggcttcttct tatttagagg attggatttg aaaagacatg agaattcttt gcagatacaa
    18421 gatttcgtct gactcattag gactgatttg ttttgaaata aaccagaaat caaaaaatgg
    18481 atcaactgat aaattccatt ctgtatttct atccattcac aatagaaata aagagataaa
    18541 aaaaccaaga aggctttcat cgaaatctct ttttttagat ctgtttttat agccttggtc
    18601 ttggttgatg cacaacccca ttctaagttc tgctaaaaat catagtatta aaaagggaat
    18661 cgagtagaca aatagatgct ttacctgaaa tgttcctatc atagttattt ttttaaattc
    18721 ctaaataaag agagacccta aatggacagt aaacacatat cctttgacaa tccattggca
    18781 catttgaacg aaccagctcc aattccaacg aagactggta tatacgaaga agccctgaaa
    18841 atgggagaag aattaatcaa aaagccactc gctggtggtg gaaccgatcg aatccttgtg
    18901 caacattcca aagacagaat gacagtctgg gaaagaatta aagttctaac tgataaagag
    18961 ccaaccatct tttatcaaaa ctggggagcc aatttagatg gtgcttctct tgttactgga
    19021 attttaaaca ttggtggaag agatgttgct atctatggac atgatttcac attacgcgct
    19081 ggatcaatgg atgcgactaa cggtagtaag ttagcaagac tcatctatat ggcaggcgag
    19141 catggaattc cgcttatcgg tatgaatgat tctgctggtg ctttcgttcc tgctggagtg
    19201 ggtggactag acggttatag tgaagccttc acagcacttc gaaaaatttc tggtaaagtt
    19261 ccaagtgtga tgctcatgtt tgggtttaat gcgggtggtg gttcttatct tccacgtcag
    19321 ggatctttta tgattcagtg tgataacaca ttctttggac tcacaggtcc tggagttgtg
    19381 aaatcagtgt taggcgaaga tatttctgca gaagatttag gtggaccaaa agtgcatggt
    19441 caaagtggag ttgtggattt agtaactcca gatgaatatg gttctttgaa aactgcactt
    19501 cgtctacttt cttatctacc ggataacaat ggagtaaacg ctcctttctt tccaacctct
    19561 gatccacttg atcgttttac ttatgaagaa gatatcttat ttaaaaagac attcaattcc
    19621 ccgactggaa tgaacactcc atttgatatc actttatata tgcaacaaat ctgtgaccat
    19681 ggttcttact ttgaagtgca accgcaaaga gctagaaact taatcactgc atttggaaga
    19741 attggtggtc atgtggtagc attccttgcc aataactcag ccgttagttc cggacagatt
    19801 gatattggag ccgcaagaaa aggaactcgt tttgttcgtt ttgcaaatct ttacaatatt
    19861 ccaatgatct ttttagaaga tacaactgga ttcttacctg gtagagagca agaagcaggt
    19921 ggtattgtgc tcgaaggtag aaagctactc gattctatca ttgatcttcg cactccacgg
    19981 ataacattaa ttatccgcaa tgcgtttggt ggtgcgtatg ctactttcaa ttcttatttc
    20041 acaggagcag acgttgtgta tacattgccg actgctcgta ttgctgtgat gggtcctgcg
    20101 ggtaaagact atgtttacaa agatgaaatt tctaaaataa acaaggctta tgcagaggca
    20161 atcaagaagg gtggggcaga agatgaagct aagaaagtga gagatgcaga acttgctaag
    20221 ctttctcagc tctatgaaaa agaattaatg aatccaaaag aagctctttc tcttggttct
    20281 gtttcgagtg tcgttattcc tggaacaagt agaaaagttt taggaaattc tttgaattat
    20341 ttaatgagtc gttataaagc atctccaatg ggtggtgtgc aaagggagtt tgaataatgt
    20401 ttgatgtaac aggaaataaa atgaaatttc acgaatcctc ttccgagtgg attcgttctt
    20461 ttgatctaag tgggatcaaa tgtcttatcg tatgtcgtgg tccggttcgt aaagaagcaa
    20521 tggatgtatt tgatgaaatc ggaattaaag aatatggaat tctactttca gaaaaagata
    20581 gcgttgttta tccaatggca tatgcacccg agcttagaaa ctttcgattt atcaacaaca
    20641 ttcatagaat ctctgattat atgggagctg ggaaagaaga gaaagataga attatcaagc
    20701 aaatcataaa cattgcggtt accaacaatt atactcacat ctttgctgga tatgggttca
    20761 tggcagaaga cgctgagttt atcgaagcaa ttgaaaattc aggcgttaaa tttatgggtc
    20821 ctgcttctag cgttgcaaag caagcaggag caaaggatgc tgcaaagatc atcgctcgta
    20881 aattaggagt atctgtaaca cctgggattg ataatatcac agctcttaca cttcttagaa
    20941 aagtgggtgg aaaagatgga cttgcaaaga ttgcaaaaga gaactattta aatttttctt
    21001 atgacaataa tctttctgag tctgaaaatg cagataaact cttgcaacta tcgtatgatt
    21061 ctcatattga tatcatcaca attgaagacc tacaaaaaga agcagaaatc gaatcagaag
    21121 taatttggaa acaatatcca aacaaccgta ttcgttttaa atacatcggc ggtggtggtg
    21181 gaaagggaca gcgtgttatc aaaggcaaag aggaagtaaa agcagctgtt atggaaattc
    21241 tagcagaatc caaagtaaca ggtgttggtt ctaacagaaa cttcttgatt gagttaaaca
    21301 ttgaaaacac tcgtcacaat gagatccaat tgattggaaa cggtgaatgg tcgttagccc
    21361 ttggaggaag agactgttct ttacaaatgc acgagcaaaa acttttagag atttctaata
    21421 caagagaaat gctagaacaa gaaattgctt cttctaaaga tccaaaaaga gctgaaactc
    21481 taaaaggaga tctaaaagtt ctcacagaaa tggaagagca atcggaaaga tttggagccg
    21541 cagtaaaact agacagtgtt tctacttttg aatcgattgt agaaggaacc aatcacttct
    21601 ttatggaaat gaatactcgt attcaagtag agcatagagt aacagagatg gcttataaat
    21661 taaaatttac aaatccatcc aatccaaatg aatttttcat tgtagagagt ttaatcgaag
    21721 caatggcaat gctttctgtg catggcaaaa gagttcctaa gccacaaaga attttaagaa
    21781 atgtttctgg ggcagaagtg cgaatcaatg caactaacca agcactccaa ccacacgcag
    21841 gtggtctcat tcaaaactgg tctaaaccca gtgagtttga aattagagat gaccaaggga
    21901 ttggaattcg aaacccagat acaggagcct ttatccacta tcgtcttgca ggagcttatg
    21961 attcaaacgt agcccttctt gtgagctatg gaaatagtcg taaagaaaat ttagagagac
    22021 tccgccatat cattaaaatt actgagatga gaggaaatga cttacaaacc aatatgccag
    22081 ttcactatgg actgatcaat tggattttag gtcatgatgt gatgtttaag ccaaacacaa
    22141 aattcatgct ctcatatctt gcggctgtcg gttctcttgc ttctttctta agagacattg
    22201 acctagaaat tctctggaca gaaacagtga agaaaacttc caaagaagac ccagctcttg
    22261 gtaaagctct tgctaagaag gtaacactga ttacccgccc gattaaacta ttatttaata
    22321 gtccacattt actcgcggga tgtattggtt tcaatcgcca tagatcttgg aagattgtag
    22381 atggaaaacc agaatttact cgaaatccac ttgaaattct aaaagaactt tatgaatact
    22441 tagatctaga aagatcagaa ggcaaagctc cttgcgatga aatttgggat catgatgaag
    22501 aaattttaga aactggtctt gcattttata gagatttaca aaagatttct ggcaaaaacg
    22561 agccgtattc tttctttgat gacgcactta gaaacaataa aaatcctgca ccggataaga
    22621 taccggctga cgtttgggaa aagtgtatgg gatctcatgc agcattccaa gttggtctcg
    22681 atctaattaa gatcattcca aaagtaggaa gagcatctgg attctttgaa attagaatga
    22741 atgaatctac actggaagca gagattcctg aaaaattcac aaacgcagaa agtgtaacta
    22801 aacttgtgaa gtttttagct ccacctccaa aagcgaagtc agacgagatc atagctccaa
    22861 tgggtggaat gttctatgcg agagaggctc caaacatgcc tcctatgatt aaagtaggag
    22921 atcatttcaa tgcaggtcag ccactcttta tcatcgaggt aatgaagatg tttaataaga
    22981 ttgttgcgcc attcagtgga acaatcacaa aaagcttcat ggaagacgca gatggaaaga
    23041 tcattcaaaa aggacagcca atctttaagg tgcagccaga tgaagtgatt gtagaagaaa
    23101 cagaagagca aatctacaat cgcaagaaaa aagcaacttt agagctaatg gcttagggta
    23161 gttaagaacg aagaaaaaag attaccacag ataaacaccg ataaaggatc gatacagtct
    23221 atcgtttttt tatcggtcag ttatcggtga cctcggtggt aatttctgtc tgatttattg
    23281 acatcgcctc tatcgctaac tcttgtaaca atcaaatcaa atcccaaaaa ggaaaattac
    23341 tatgaaaaaa attatctcta tttttttatt tgcaatcgca tattcacaat tagccgctca
    23401 agcggtaaag aaagactatc tttcttataa ttgggtaaag tatttttatt ctaaacaatg
    23461 gaatgcgtat ggggaagaag tgaccaagca aaaacaagaa gcagagaagt taaatgcaga
    23521 aggttttaaa ttgtataaag ctaaaaaaga tgcagaggca atcaagaaat acgaaggagc
    23581 aattgatctt tttccaagtg gagagattta ttataattat ggaaatagtc tttcgaatgt
    23641 aaaacgctta gaagattctg ttaaagccta tgagattgct ctcaaattag atactcctcg
    23701 tcctgagctt gctctttaca atattgcttg tagttatagt agattagata aagcggataa
    23761 cgcttataaa tacttaggaa gtgcagtcca gagaggatac aatgcatttg aatacatcaa
    23821 aaaagatcct gacatggcga acttaagaaa acaagctgac tgggaaacaa aactaaaagc
    23881 aatcaaagcg aatgcaaatt atgatgaatc agccctcaat ggaaaaatta cagaagaagg
    23941 tccaaggcag cctgaatatt tttatctatg taaatctgga attgctttga ctgtcggcgg
    24001 ggatcaactc aattgtgagg gaaaaggatt tgatcgagga aagtggaaac tagtaaacgg
    24061 agatgttaaa atcactttct ctgaaacatg taatcctgaa tatgtagcca cagaagcaga
    24121 gagaaaaaaa agagaaacag aaccacaata cgaaggtcaa tgccctgtgg gcaaaccgaa
    24181 gtttgccgct tgcaagaaag ttgattccaa agaatactac tcaaaccgca gttttacaag
    24241 agaggacatt cgaccaatgc ttcttcctac atctgatgat cctgctggta tggttgggat
    24301 gccactttta ttttctaaat ttaaaaatgg acaagaacca aagcaatgcg atccaaattt
    24361 taaagtaaaa gaattaaagg attataaagt agagtaggct tcggagattt gaaaagattt
    24421 atttaatgcc agttcgacgt aacagaaaaa ttaaaagatt accgccgata ctaaatagga
    24481 taatttaaaa ttatccatcg tttcttcatc gtttatttat cggtgccctc ggtggtaatc
    24541 agttgaaaat ggaagtgaat ctagaatcgg gtatatattt gttctgtaga ttagtcttta
    24601 aatttcgtat agaggtaata ttctaactct tgcggttgtc gtatctaaga ttacatgtgc
    24661 tccatctttt aatgctattg aatgtaaatt taaaaccttc aatagttgat ttcctaaatc
    24721 ttcaggtaaa gagctttttg ctctaattag aactacactt ggtttttctg aatttgatag
    24781 agctaaaatt gtgctgaaat ctaaatcttg tgttaaaatt atgtaattgt tttcttttgc
    24841 ccaattaaaa aattccttat cagtcgcatt tatgtttcct acgcttgtcc aatgaacgga
    24901 ttcaatttca aattctttaa aaaattctac ccaagcgggt gagagattca tatctatgag
    24961 tattttcatg cagccggaag ttctgtttca aattccatta atctccaagc agcatatttt
    25021 aaagactctt gaatatcttc taattccaaa tacggaaaag ctttcagtat ttcgtcattc
    25081 gttttattcg atgcaagtaa tccgagaatc attccaacag tgacccgcat accgcgcacg
    25141 caaggcttac cgcccattac ggatgggttt aatgttattc ttgagaattg tttcatactt
    25201 ttctttatat aatggaaata ttcaatttgc aagaaaaatt taaaattcga tattctattc
    25261 atttatatac caaaggacaa aagtaatttc ccacaaggat tttatttgag tagcatttgg
    25321 tataccgaac ctactgatgt tcgggaacac taagaagtgt ttagaccaaa tgcatatttt
    25381 tatttttaaa atcggacttt tcttttagcg tttggtataa aataaaagaa aatcaacaag
    25441 atgattgcga aggttactaa taaagttact taattttaaa attatcaatc ggttttggtt
    25501 cgttcattta tcggtgccct cggtggtaat cagttgaaaa aggatgcttt gaatagtgat
    25561 ctactcccac aacattcgtc ttcatttatc aaataaaatc tgcttacaga tatatttgta
    25621 gtagatggat tcaaaacaac tctttagatt gggattcatg caatcgatac gatacatact
    25681 gattccattc atttttggtt tactctttct aaactgtaat aaaaaaaatc aatcaccgga
    25741 taaaataata tctaatatag caactatcga ttctaacttc aaaacaacca atctgacacc
    25801 tatattagaa tatatagaag ataaagaggg gaatctttcc tttgaagaaa ttcaaaaaag
    25861 agagaaagac tggttaaaat taaaaaatga tgagtttaca aattttggat ttaatccttc
    25921 cttcttctgg attcgtttta ctgttgaaaa tattacaaaa gacaagcaaa gaccgatttt
    25981 tgaaattagt tttcctcgcc aggattatat cgaattttat tctgaaacct ctggaagata
    26041 tgaaacagaa attacaggag atatatttcc atttcactct cgtaagatca atcatcttgg
    26101 ctttgcattt gaattagaaa tagaagcaaa tacgaaaaag acttactttc tccgaacaag
    26161 aagtatttac aatcgatcta ttttaaatcc acatttacat acccagatag aatttcatca
    26221 aagggcatcc gagagaaatt tggtaaatgg aattttgtat gggctaatgg gaattatgct
    26281 cttttataac ttttttcttt atcttacgat tcgagaaaaa gtgcatttgt attcttctgt
    26341 ctcgattgta ttttatctac tcatcacact tgggattcat gggacatcgt atcagtattt
    26401 gtatcctgat tttccttgga ttcaaaacag agactataca atctttactc cgcttgtaac
    26461 cctctttatt gttttatata cgaatgagta tttagattta aaagtaactt tacctaaagt
    26521 ttataaaata ctaaaacttt ttaatgcctt tacattgggt atggcattta catttccatt
    26581 tttagtagag caattttcaa aattgatttt ccaatttatc ttaaactttt gctttttctt
    26641 tgttgtattt gcatttttat taggagctta tttaagcgtt aaaagatata gacctgctta
    26701 tttctttatg tcttcttttt ctgtattttt tgttagtatt gtaattgctt ttctcactgt
    26761 gaatacattt ttacctgcta gttttttggg gtattggtct tttcaaattg gtttttcttc
    26821 tatgttaatt ctacttgctc ttggaacagc ggataagatc aattatctga agaataatct
    26881 aaaagaagca aatgaatttt tagaatataa agtagataag agaacccaag aactaaaaga
    26941 agcaattgca aaaattaaaa ttgatttgaa aacagcgagt aagattcaaa agaatatttt
    27001 acctaaaaat aaaacttcca ttcaaaaact aaaaatctca acggaatact taccgttaga
    27061 ggaagtaggt ggggattttt atgatattac agaattgcaa aatggagtga ttcgaatctt
    27121 tttagcagat gctacagggc atgggattca agcagcactt atgaccatgt ctatttattc
    27181 tgaataccaa aatataaaag agtatcaact cgatgcagga gaaattttaa atattttaaa
    27241 taaacaatac tactatcgtt attattattt aaatgcctat tttacttgta ttttagtgga
    27301 tattgattta gaaaaaaaga agatttatta ttcgtcggct ggtcacccgg atcagatttt
    27361 atttttggaa aacaaaacta cattacttaa aagttccggt aagttgatgg gagtcttaaa
    27421 agatgtattg tataaaacct gtgaagaaga ttttcgggaa ggaagtagac ttcttttatt
    27481 tacggatgga attttcgaag aattaaataa agaaggagaa gaatttggag aagaaagatt
    27541 tttagaaggt gcaacggcta atcgatattt gcccaatgaa gaatttcccc aaaagacaat
    27601 tcaatccgtc tttcaattta tgaatggttc tagtttacaa gatgatataa cgcttcttac
    27661 gattgattat tgaagataac cacggatggc taaaaaattt catttttatt tattgcttct
    27721 atttagttta cgaacttttt cagcagaaaa tccatctcta aaggataaag gtttttcttg
    27781 aatggtttta tttaatcttt tcagtttagc tagtttaatc cattcagaaa gagctttttc
    27841 gatagattct gtaatatttt taccaccgct aaattctttt acatcattga ttaattgatc
    27901 tggtagaatt gctgttactt tcatcgtaat ataaatacga tactgtatcg tatttgtaaa
    27961 gaaatatttt accacgaagg gcacgaagat cgagaaggtg taagtttttc atacggttga
    28021 aacttctgtt taaaattatt gtgagtcttt tagaaaaaga tgttttgaat taaacggagt
    28081 aaaatttgag atattatatt ctgattcggg aaaccaaggt tatgtaacct cgccttcgca
    28141 taatcataca aagtatcacg ttaagaatgt ttttgggtaa aattataaag caaataattt
    28201 tctttacaaa cttttcgaat gctgattcaa ttagaagaat gattaccaag aagggggaac
    28261 tattgaaatg agcgagttac aacagctaga ttttaaatca gtagagtcaa ttttacaatc
    28321 ccattcgatt gagcatttag atatgagaga aatatctaaa ctttttatca atgaaacatc
    28381 tattacaaac tatcgtcctg aatctgtatt tcaaattgat cctagaaatg gagaattgat
    28441 cgtttacaat tcttctcgtg ctacaagacc gaacgttgga cctgatggtc ttgaagaaaa
    28501 gaaaaaagaa gctgagcatt gtcctatttg cgaaggaaag actacgggga taatggatgt
    28561 tacccacatg agtgagggtt ttagttttat caataagaat atgtttccaa tattttatcc
    28621 tcatgatact gatgtgcccg gaaatatggg ttctgtttct tttggacttc attttctaca
    28681 gtggacttct tcttaccacc atagagattg gcataatatt tctcataaag atgctgtgat
    28741 cgtattatct agattagccg cacttgagaa aaagttactt gttgaatcga gtgacttcat
    28801 gcctggatct attgcatcgt cacaagagaa gccaacctat ggttatgttt ctattataaa
    28861 aaattatggg aaagctgccg gtggttcctt acagcatggg catcagcaaa ttgtatcttc
    28921 gaatattatg ccgaaacaat ttttcaataa ttggagtttt agagaaagac atggagttca
    28981 tttttccaga tttttaatgc aagagaatcc agaggaatta actgtaaaag attatggcaa
    29041 agtagtgttg gttgttcctt attttatgag aagacccttt gatatgtttt taattgtaaa
    29101 agatacttct aaaaaataca tccatgaatt aagccaagaa gaattagagg gagtcgctaa
    29161 aggctggcag gatgcaacaa gaatcatgct taaaattctg aagcgactta ggaaagaaga
    29221 agcgtataac gttactgtga ataatggacc gggagcaggg ctttatgttg agtttttacc
    29281 atttacacaa ctcattggtg gctatgaaaa aattgggctt tgggtatgcc aagaaagccc
    29341 attcaaagca gccgaaactc ttcggaattt tataaatgaa ttggaaccac tctgattaaa
    29401 agaagaaaaa cttttttcca cttttaaaga taaggctact aacggcttcc cagttagata
    29461 gatttggatc tggatttttt ccatctccca ttcggagtag ttgttgctga taccaagcaa
    29521 tttctaaggc acctgcttca tctactacac cgataccagc cgttacgcgc tttagaacca
    29581 ttcccatgga ttctcctgat ataattttgg atggaaactt tggatccatt gcaattggtc
    29641 tagcgagtcc caatacatca attccttctt ctgttgcctg atccattcct gctcttgttc
    29701 taaaaccgcc tgttagcatc ataggacagc ttacagttct tctaagtaaa tgcgcaaatt
    29761 ctaaaaagta tgcttctcta agtcgggttg tgtctttttc atgcactccg gtcatagctg
    29821 ctttttcgta agttccaccc gagacttcta aaagatcaat cccttcgtgg tctaacgtct
    29881 ttgctacctc tgtgctttcg tcctctgaaa aaccgccatg ttgaaaatct ttcgaattta
    29941 attttactgc aattgcaaaa ttttctccta cagctgctcg aatgcttcta tatacttcca
    30001 ttaaaaatcg cattctattt tctaaacttc ctccccattt atcttttctt tgatttgtaa
    30061 gaggagatag aaattgattg atgagatatc cgtgtgctgc atgaatttct acaccatcaa
    30121 agcctgcttc ttttagtttc aaggctgttg cggtaaatcg atagattaaa tctaaaattt
    30181 cctcttctct taattctctc ggagtagcaa aaattccaaa tggagcgtta actgctattg
    30241 cagatggtgc aacgggttgt ttagataaaa gaacaggaga ttgtcttcct ggatgattga
    30301 tttgtccaaa tagaatggaa ccatgctgtt tggcaattct tacccattct ttaaaagggt
    30361 ctaaattggt agcgttttcc aatactacat tgtttggttc ccctagagcg gtcttatcta
    30421 ttatgatatt gcctgttatc aatagacctg ctccactaga gccgaatctt tcatacaatc
    30481 gatagatggc tttatctgga agtaggcttc tatctgctat attttcgctc atcgcagctt
    30541 ttgcgaggcg atttggaatg attaggtcat ttttaagttt gagaggtcta tttaagtttg
    30601 gcataattaa ttttctatat gatacatttt tttaattagt ttgtgaaatg gtcttccttc
    30661 tttaaacaat ccatagtata gaatcgcaaa ggatatagga agtcctagaa agtaacttag
    30721 aatgtgcatg tttgtagagc caccggttcc cgcataacgt gaaaatggat cacctgcacc
    30781 tatcaataga cctgtataat aaactgtaat tgcactgatt gcggcagaac caaagccaac
    30841 taagaatact cctactagat aatttgcagc aagtgcgcct gaggaaacta ttttcttgga
    30901 gaaagtagat acactttcct ttgtccactt tgaaaagtaa taaagaattg ctggaactaa
    30961 aaacactcca tcaatataaa gagtcattgc tacagtagaa gtaaacatag cagcaacgct
    31021 tccgattcct ttatagcctg caccgggttt ccatgcttct ccgccaaata aatctctgtc
    31081 gtaaagaaat ctatcatatc ctaaaccatc ccatccataa agaagaataa agaacattcc
    31141 gaagtatcca aaaatccaat ttaaatggga aagataatat ttttttcttt taattaattc
    31201 agaagttacc caaaaaccta aaattccttg cgtaacgttt gtaattccaa agccaatgat
    31261 gagccagacg ggtatagaat acaaggtctc tgcgacttgc atcgtctccc agcttgggtg
    31321 ttttaataat aataacattc ctgttggtgc ccatatacag gaaagaacaa ctaacgcttt
    31381 cgcgtagaag ctggtagata gaggttcctt ctcttcttct aattttttag aagcggctgc
    31441 agccatagtg ctaccgattc catatgccca aaatacatct acttgaacca tttcattctc
    31501 cttatttatt tttgtatgcc ttgtaagatg atggagataa tcactgttat ccactcttta
    31561 cttgctttaa ttctttgttt tttattttcg attggtgaaa cttcttttat tccttcgaat
    31621 ataactgcat tgattgctct tgccgtaatt tttatatcta agtcttttcg caaaaatcct
    31681 ttatttatac cgtttttcaa atattgagct gtgtattcat cgaaaatctc catggagtca
    31741 gagatctttt tttgactgct tgcaactcct tgggattcaa agaatagaat ccgagccatc
    31801 gagtcatctt cagagaaaag atcaaagagt ttttccccaa ttctatgaag ttgttcttta
    31861 tactcctcta aattggttgc gccatttggg ttttcattga ttgcgatgct tacaatttct
    31921 tttagtattt cgtttactgc ggaatgaaat atatcttcct tgtttttaaa ataacgataa
    31981 aaagttccat gtcccattcc tagttttctg gcaatatctg caatccctgc ggcatgaaaa
    32041 cctttctcag caaagacttg aacagatgca gctagaatgt cttttttctt tttatcgata
    32101 tctgtcattt gtccagtaat gacatgtcat tctgttaagt caatagaaat taaagaacat
    32161 tgttaatttc aagagaaatg atacatacat catcttcaaa ttgattgctt cctctaaatc
    32221 tttgtagcgc taaaaatagt tcatttagaa aggtttgccc ttttagagag gagagatttc
    32281 ttaacgtctt ttccatttca ttttcttcaa agaaaattcc atctccagaa attgatctgg
    32341 tttctgtgaa tccatctgta tagagcaata gtttactatt tgcatttaat tttatttcat
    32401 gattctggta ttttttattt cggtctatta attcatgatt ttgaaatatt ccaatggctg
    32461 agcttcttcc acctgagaga taacgtatat tttctccctc tactatgatt ggccaattat
    32521 gacctgcatt cgaaaagaga agggagttat cttttatatc ataaacacaa taaaatccag
    32581 taaggaaaaa accacctatt tgattaaata aaacttcatt taaataaaat aaaaattctg
    32641 ccggattttc tttcctgatt ccagactgta aaattgcagt ctttagcata ctagttataa
    32701 atgctgctgg aactccatga ccagatacat cacttaaaaa aattccaagt ttatttggat
    32761 ctctgaaatt gacaaaatca aagaaatctc caccaacatg catcataggg tgatataaaa
    32821 aactaataaa gggataggga tttgcctttg gaattagttt attttgtatt tttcgagcca
    32881 tatttagatc agtgtttaac gcattgtaac tctgttgtaa tttatttctt tcattttcta
    32941 actcctcatt ttgtttttgt aattttcttt ggaggacaat tatgattgaa taaatcaaaa
    33001 tattcactat atgtgtaagc ataaaaccaa ggataagact aaaccgattg atcttttctc
    33061 cttcttgcaa aaaattatag tattcaattg aattggtaaa gagtaaaact agaagtaaga
    33121 gatgtaggaa taaacaatat aaaacacaaa ataatccctg tcgtattttg gggtttaagg
    33181 agcagagagc tgtagcgtat attgtccctc ccaaaaagaa ggaattcatt ccagtcaaat
    33241 aaacaaaaaa atatataatc gttaaatcaa tagctgaaac aaaaaaacta aaattactaa
    33301 atcggtttgt taaatcaaaa aaagtttcta caagtaaata ggttaaactt gtaaaaacta
    33361 gaagtaagct tatatcaaaa atcgctgtat aggaataaaa tttcgcaata atacaaccag
    33421 ccgatgctag aagcaatcta gaaatgttaa ttgcaaaata ggtataggaa atttttagga
    33481 ttcctctttt tctgaggtct ggataatact tttccataaa taatgtttta caactttaaa
    33541 taaggactac tttttacgac aagttaaatt ttctgaatag aaatcattca atcaattcta
    33601 gaagaaaatt ttgattgaac tcacataact agataagaca agcattctat tattttataa
    33661 aatggatcaa tagagaaagg attggaatct ggtaagatga gtagttttgt gaaatcatta
    33721 acccttgtag attagaattg aatcaaaaaa ctattctaaa attcccagat cttctgcttt
    33781 tcgtaacatt tgcaatcgat tgtttacttc taatttctga taaatccctc taatatgaaa
    33841 acgaattgtt gagatactta ctaaaagcaa cgcagctatt tcagatgggc ttgagccttg
    33901 tgctaattct tttaatattt gtaattcacg attggataac tcttccatgt tttctggttt
    33961 ggattctttt ttttgctttt gaaaataatg cataacacgg actgcaatgg atggagtcat
    34021 ataggctcca ccttctataa taatatctat aatagaggaa actttttcag cttcactttt
    34081 taagatgtat ccaatacaac ctaactttaa acattgaaaa attgtatctt ctgagtcaag
    34141 tgctgtcagc ataatttttt tactagatgg attgatttgt gaaattgttt ttagaaaatc
    34201 gatcccactt gctttcggga gtaatatatc gatcaaaaaa atatctgatt cttttaaatc
    34261 tttttctgaa cattcctcaa cggagtgata acatagaact ttttctattc tagaatgttt
    34321 agataataaa tccattatat gattacaaaa atatttctta tcttcaataa tagttacttt
    34381 cagtttagca ttcatgaagc caatactttt gattcttcta aaaaaagaaa catttttaga
    34441 gaaaaaacga tacttcctat tctagattaa aaatgatttc aaatcggaat aaagaatgat
    34501 ttgcttgaat cgttagtatt ctaacctaca gaaatcggga aggatttgat agaagtaaat
    34561 tttttaattt agatttagtt ctttttatcc tcctattaat ttgcataagt cggttgattg
    34621 gaaatcttgc attttcttag gtgctctttt aaatatttga ttttaagata gcagtcggtt
    34681 tctaaaatcg aaatgttttt attttataaa aatatacttt caatgaaaag ttaagatagc
    34741 aaatcaatga attggaatat aaaaaatttc ttattttatc ataaaatggt gaaaaaatgg
    34801 ctttattcac tttactttga ttctttggtc tagaatcctg caacataaga gtctatgatt
    34861 ttcttaggac gagaaaatct taccgcacca aacaataaaa gcgaatcatc ttaatcatag
    34921 gatttagatc atttaccata tacttctttc ataccaaagg gtgaaaggat gagtttttag
    34981 aggagatatc tatgaaatta tcaattaaaa atactattat acttagcttt gcaactttat
    35041 ttatactgat tctatttatg gcattattga ttatttttct ctatgcaagt cagaatgatt
    35101 tatttgaaag ccagcaaagg aaatttaatt cttatctttt ggcagatgaa ttaaggcaga
    35161 gttcagatga tctgacaagg tttatgcgaa cctatgcttt aacaggcgat ccaaaatata
    35221 aagaatattt ttttagtgtt ttagatataa gaaatggaaa aaagccaaga cccgaaaaat
    35281 acgataggat ctattgggat ttttatacgg tagacggaac aaagccaagg caagatggag
    35341 aaacaatttc tcttctaaat ctaatgaggc aaaatggatt tacagaagaa gaatttaaga
    35401 aattggatga agcaaagaaa aattcggatg ctttggttca tacagaagaa attgctatga
    35461 aagctatgga gggtaaacta gaagaggaaa ttagaaaaat gattcaacca aatgaatcac
    35521 tttctgaatt cgcaaagaga attttacata gtgatcaata ccataaagat aaatatagga
    35581 ttatgaaacc aatagatgat ttttatgttt tattagaaca aagaacatca agagaagttt
    35641 tagaaaacaa agaaaagcaa actcttcttt tgcgaataat tctgggaact gtcgtatttt
    35701 taataatcat actaatttct gtttcctaca caattcatat aaaggtaaca gtcccaatcc
    35761 tgaactttac agatgaaatg gggagactgg ctgacagcaa agacttaacg tattctcttc
    35821 ctgcttcaga aaaaaatgaa ataggtttac tagcaaaagg atttaatgga tttctagaaa
    35881 gcatacttct actctttaga gctttttcta caaacatcaa taaagtaaat agtttgtcga
    35941 agacgttaga tgaaactatt caagaaacaa aaagatcaat tcaagaagtc tcggttgcaa
    36001 cagataatgt ttcttcagag actggacaac ttatggttta tacagaaaca atcgtcaata
    36061 tggtaaatca atcaaaagat aaaatttctt tcagttcgtc tatttctaaa gacaatacaa
    36121 agaatacaaa ttttttatta gaagaaatta ctcaaaccca cagagaaatt gaaattgcaa
    36181 ataaggagtt aaaggaaata gatcgagaat tagaacagac tgcaaaggta acagaggctc
    36241 tttctgataa ttcaaaagaa attaattctg ttttgagatc agtaaaagaa atatcaaagc
    36301 agactagttt acttgcgctc aatgcagcga ttgaatcggc tagggcaggg gagcatggaa
    36361 agggatttgc cattgttgcc gatgaggttg ggaagcttgc aatgcaaaca aatgaagcaa
    36421 cagaaaaaat aggaagagtc atttctgaaa ttacaaatga aattgcagga actgttcaaa
    36481 aaattagaaa ttctcatgcg agtagtcaaa gtttgattca aattattcaa agaatagaaa
    36541 aggttatttc taataatttt gaagcggtaa aagaatcaag gacgcaaaca gaaaaagttt
    36601 caaatgctat tttggtttta gaaaagaata tatttgaagt aggtgaaatg acaaatgaga
    36661 ttttcagggc taatcaacat ttaactgcat ccggtgaaga ggtgagtgta gccattaaga
    36721 ctagactaac gtctctagaa acgattcaag aaaaaatgat tgctcttaca aaagaaacag
    36781 ctaatctaca aagggattta aatttgttta agatttaaaa ctgatcatag ctaaaatttt
    36841 ataatcgact gatgcggaga atctttttac aagagttcaa ctagtaatct gttttagcct
    36901 atctgcggga acgataggca ttcaatagag gttgtttcat ttcaatgaat gcagcaatga
    36961 aaacactgct aaaacaaaag tttctcactt ttgatcatta cgatttagat aatataagaa
    37021 ctgttcaata aatgtaaata aagcaataac tctctagatt ctttttctta gttccttttt
    37081 gtcaaatttt ccaacgctcg ttttgggtat aacgtcaaca aagagaatat ctgagagttt
    37141 tggaagttgc caatgggcaa agtttttact taaaaaatca tgaactgttt tagaattcac
    37201 tcttttattt tcaggttttt ggatctcctc ttgtgtaaga actataaata caacaggcac
    37261 ttcttctcga ataggatctg gtttaccgat agccgccgct tctaaaactc ctggaatagc
    37321 cattgctaaa ttttccatat ctacactaga aatccattct ccacgacttt ttattaaatc
    37381 ttttttgcga tctgtgatct ccataaaacc atactcatca atttttacga catccccggt
    37441 tctaaaccaa ccatctgttg taaaattagt attcgcattt tcgttattaa aataggattt
    37501 tgctatccaa ggacctctga ctaaaagttc gccaaccgtt tttccatccc aatctacttc
    37561 ttttccttcg tcgtcttcta ctttaatttc gacacaaggc acattcatac cttgtttggc
    37621 tctccaagta atttgttttt ccaaatccca agagtccatc tctgacgtta agcgagagac
    37681 tgttccgaca ggagagagtt ctgtcattcc ccatgcatgt aaaattggaa ttccaaactc
    37741 catttgaaaa ttttcaatca tagatctagg gaccgccgac ccaccgataa ccattcgatt
    37801 gagtgaacta agatcatgct ttttagattt tagatgctga tagagaagtt tccaaatagt
    37861 aggaactcct gctgcaagtg ttactttttc tttttcgata atttcagcga gaattggtcc
    37921 gcctagattg ggtcctggaa aaattaagct agaaccagtc atggcagctc catatggaat
    37981 tccccaagca tttgcatgaa acatcggaac tactggtaaa gctcgatctc tttcggttaa
    38041 cgcaagacta tcggcaaagc atattcccat agaatgcaaa aagaaagaac gatgactata
    38101 tagaactcct tttggatcgc ccgttgttcc ggaagtataa cataaacctg ctgcttgatt
    38161 ttcaggaaaa tcaatgagag gaatatcttc atttgcctca tttaaaagtt tttcataatc
    38221 aagtgcatta ggaatttcta cgccggtagg agtgttatca tccattaaaa caagatgctt
    38281 tacatttgga aatttcgaca ggagaggtgc aattaatttt gcaatagaat gatcaatgaa
    38341 aattacttta tcatccgcat gatttacaat gtattctaat tgctctggaa agaggcgaat
    38401 gttgagagtg tgcaaaacag ctcccatgca aggaatggca aaatatagtt cataatgcct
    38461 gtagctattc caagcaaatg tggcaactct atccccttct ttgattccaa ggtttcgcaa
    38521 aacattcatt agccgtagaa ctcttttgta gaagtcttta tttttgtatc tatggacttg
    38581 attgttcgat tggtaagata taatttcttt ttctggataa tttttaattg ttcttctaag
    38641 aagagaagta agagagagtg gaaaatccat cattaaaccg ttcatttttt tatttcctcg
    38701 tttttaataa aattgatatt tgcattgtct tttttaaatt ctgccttttc tccaattttt
    38761 aaaagcctta ttctttcttt tttgccagat ttttccaagg cttctaaaat catttctctt
    38821 tcgcttccac ctcttctgta aaaagtgctg tggtgaattg gaatcatgta ttttgcattt
    38881 aagtcctgaa agatttgcac tgctccgaat ggatttacat gaacagaatt tccaaatcca
    38941 ccacctgtga tccagcttgc aggaccaact ggtatgagcg caatatcaat gttatatgct
    39001 tttccaatta atttaaagat tgagtcatca tatcctgtat ccccaccaaa gaaaactgtt
    39061 acgcctttat attctataat atatccagta taaggttccc catcccagaa attgtccaaa
    39121 agccatcgtc caccaaaatg ttttactggt attgcggtaa ttttaactcc atctaaattg
    39181 aattctttca tgttttcaac actgataaca ttctcaaata acgcatctgg tatgtatgta
    39241 attccaccac ttggggctaa tatgtgtttg ctcccaatta gttttcgtag agaaggttga
    39301 tctagatggt caaaatgtgt atgtgaaatt aaaacaaagt caactttttc aagtttttct
    39361 agatcaattc ctggttcaat aaaacgctta acgacaattc ctgtatgaga actaaagttt
    39421 ggatctgtga tgacccattt atcaaagaat ctgattaaaa cagtcgcatg tcctatccaa
    39481 taaaattcca gtttttcagg actattgatt ttttgtggtg cgggaaagta atttggttta
    39541 atagttttat aaaagagagt gcatgaagat aaagtgaaaa tacatataag aatgaatcga
    39601 atcatataaa ccaattcaat gaattaaaaa aagcaacgca atcaattaat aaataaaaac
    39661 aaaattcttg ctattttttg tggtttaatt tttctatttt tatgtccgaa aaacacaaag
    39721 cagcaatatc tacttataag aaaatatata aaaccttcat agtaacagtt attttattaa
    39781 tcgtttcgag tcaggcaatt actcagtttt ttatctattc ccaaaaattt gattcgaatc
    39841 gaatcaatat tgctggtcgt cagagaatgc tttcacaaaa cattgctaaa acaattttaa
    39901 aaattcattt agatcaaaat aatccggacg caaatgattt atctgagcta gaaaatttaa
    39961 aaaatctttt tatgacatct gattatgtga ttcaaaatgg aagtaaaagt ttgaatatcg
    40021 atgcgaatca gaatgattta gcacaacaat tatttaaatc aaataaacct cattttgatc
    40081 aaattatatt ggttgctaat acgctcctac aagacaaaaa aatcgataaa gataagataa
    40141 agactttgtt tgaatcagag aaagaatttt taaaaaatat ggatttgatt gtaactgctt
    40201 ttaatgaaga aagtattaaa aagtcaactc ggcttcaaat cattgagtct ctactcgcca
    40261 tttttgctgt aattattatt ttgatcgaga ttggtgtatt atatcgacct ttgattcaaa
    40321 cattaattaa ggacaatgag gaactaagta aacaaaagaa aaatttagaa gaattttctt
    40381 ttgtgttatc tcacaaatta agaaagcatg tagcaaactt attgggtttg atgaattcga
    40441 taaatccaaa taatttatta gagattcgag aatactttca atatgtaaga cagtctgttt
    40501 atgaacttga taccgttagt cgcgaattag gtgataaagc aactccgtat gatttaaaaa
    40561 tagatatgaa ctcgataacg attgattcga caaagagatt tgaaactatt gaaacactta
    40621 aaactattct gctgatcgat gatgataaaa taacaaatat tttaacaaag agaattttag
    40681 ttcaatacaa tcctcaaatc aaggtagata catttacaaa tccatacgag ggtcttcgtt
    40741 ttcttgaaaa gttaaaagaa aacaaaggag agtttccaat tgtatttctt gatataacta
    40801 tgcccgaaat gactggttgg gaatttttag ataaactggc tggtttaaat attaatcctc
    40861 ctgtttatat actaacgtct tctattgata ggagagatat tgaaagagct cgtgtttata
    40921 agaatgtaaa gtcattctta accaaacctc ttacatctga gaaaatgcct acaatactat
    40981 gaaatatttt ttgattcttc tagcttgggg aatatttgga ttagccgcag atgaagagat
    41041 attgatccag aggttttcaa ctttagtaga aaagcaaaat tcggatgtaa aaaaaaatat
    41101 agctttagca gttgagaaat taaatggcta taaaattgag cctaattcta cgttttcttt
    41161 tagtgatata gttggagaag catcgattca aaatggcttc gtagctggaa gagtttttta
    41221 ttggaatgag gctatctacg agcctggagg agggctttgt caagtttctt ctaccgtatt
    41281 taatttactc ttattgagtg gatttgtaat caaagaaagg cataagcata gtcaaccggt
    41341 aagctatgtt ccgatgggtc tagatgctac tattttctac ggtaagaaaa atttaagaat
    41401 gcaaaatcct cactcacagt cctttcggtt ggttgccgag atcacagatt caactctcac
    41461 cttctcttta tatgggaatc aagagttatt ggagaaatat gagttggaaa cagaagaaga
    41521 ggagcatgaa ttaccaattt taaaaaaaga aaaatcttat cgaaatgcat acactgtatc
    41581 tgtttttaga aaaaaataca gaggtgaaaa tttgcttgaa acttctttac tctataagga
    41641 ttttatacct gctgtctatt tcaaataatg aagtggctgt aaatctaaat tttgagtttt
    41701 tcatgcaaat agatgatgaa gatcgattaa cactaaaaga agaggaggat gttaccttta
    41761 ttcaaatcaa gggtaatatt gatctttata caactcccga gttaaaagat gcatttgata
    41821 aattaaattt atcgcagaaa aataaaatca tcattgattt gctcaatgtc aatttcatag
    41881 attcttctgg gcttggtatt ttagtaacac aagcactcta cctacaaaaa agaaaattag
    41941 tcttaaaatt tatcaatgtc aattccacta tcagtcatgt tttttcttta ggtgggttta
    42001 ctcgttcttt caaacgtttt tcagataaag aagaagcaat gaatagcaca tgggaatttg
    42061 gagtttaata gtttcatttt ttctcacact cactctttgg ggagaggaaa cagctctctc
    42121 agaatggaaa aaattttcta aaacgaaagc ctctgaggaa ctaatggagt ttatccgctg
    42181 ttccgccact gaagaactac aattaaagaa ttgcgatcat ccaattccag agactccccc
    42241 gttttttggg gaattaggtc tttttattac gattgtaaat aaaggaaaag tgcgaggctg
    42301 ctttggcgca tttcgacata aaaatacaca gatcacaaat gttttacagg actatatcag
    42361 aggtgcactg agacaggatc caagatatcc tcctttggaa atagaagaat taaaaacagc
    42421 ttattttatt ttaacgatag cggactcgcc cgcaccagta gcggatacgg agagtatacc
    42481 gcttggacga tatggtgttt tattaacaaa ggaaaatggg cagggcgttg tttatgtgcc
    42541 tggagaaatc aaatctcatt ctagtttgaa aagaattatg caaaaagaaa aaattcaaga
    42601 tgttagtata tttaaagcaa ttttaattcg ttaagaggga gaatagtatg ttcggaagac
    42661 taagatttta tctaagattt atggctcgat tcaaaagttc gaatgccgct aatcagaata
    42721 acgtatccat gagtaatgac aaaaaattag gatcttttga taagccaaag agaggtaaat
    42781 tcaaatttgc cccacatgtc tcgaaactat ttactggctt tttcgggcat tctttctcta
    42841 gaggatccaa gagatctaat cagcagaatc tatttcgtaa tattttggtt ggtagtaatt
    42901 caaatccaat gagtgtttat tggtctttaa tccactctaa actttgcgca caacgtctta
    42961 tcttgagtaa gatattatta gtattattga catacagcat ttatccgcaa gagaataaac
    43021 tgaatttaga agagaatttt acttctaaga attattatga tggttatgta ttagatacaa
    43081 aaacaaagtt agggattgaa aatgctactg ttgaaatgaa aaatcagaat ttaggagttg
    43141 gatattattc cgttaagact gataaaaaag gatattttca aattaaagac tttatcccag
    43201 aaattaatta tcaaatcaca attcaagcag atgggtttgt aaatttcagt gcaagcaatc
    43261 tccttttcgt aacaaaagac aagcctaagt attttttgca aaaggaggga attttatatg
    43321 gagttgtaaa agactctcgt ggtaaaaatt tagaaggagt agaagtaaag attcaatctg
    43381 aaaattattc tcttcctaga agatcaaaca cagtaataac gaacgcaaag ggtttttata
    43441 aatttgaaaa attaagtgaa gggccttatt ctgtaagttt tgaaaagtca ggaaggattg
    43501 gagaaactgc taaaataaaa aaactgaaat caggcgaaaa gtttcatttg cccatgaggc
    43561 tttttaacaa tgcgtctgtt tcggggacag ttttaattga tgggcttaat gttcctgcga
    43621 ttggaatgaa tgtggtctta tcctctctac gtgctactca tagtgcatct actttttcca
    43681 atggagaatt tagaatcgaa gacattaagc caggaaccta taagttgaaa ttatcccatc
    43741 gtggatttca tgaaatagaa aaggaaaata tcgtaatcac agaaggaaag gattttgaaa
    43801 aattaagttt tattgcaaag ccgaaagagc ctgagattac agtcagcact aaccgttatg
    43861 tgttttctcc cgccgataaa atccaatttg atctcagaac atttcgtatt gagaaagtga
    43921 atattaaaat ttataaagtt ccagaaaaag agcaggcgga aatgcttctt ttacaggatg
    43981 caaataaatt aaatccggtt gatcgaaagt ttcaattgat aactgaatgg gaagaaaaca
    44041 tgcgaaactt tcaactctat gactggaatt atatgcaatt aaatcttaag gataagctac
    44101 caacggctac ctatttaatt tctgtagtgg gggaagataa gaaatactat gcgaataaat
    44161 tttttagtgt aacgtcagtt ggtattgtag tcaagagatc tcttgattct gtatttgttt
    44221 atgccagtga tttaatttct aatctaccaa tgggtggcgc gaaattctat ttttatgaag
    44281 aaataccacc agtgaatgaa aagaaaaaga cagaagaagg ggcagtaagc accgaatctg
    44341 agtcttacag ttcagaagga ggcactgagg attattacac ttatgaagta gataaaccat
    44401 cctctatcac aggttataat tttaaattga tttatgaagg tcttgctgat gaatctggaa
    44461 cctatcaata caaggcaaag aacgagaaga atatcttagt agttgcatct ggtttagatg
    44521 gaagtaatgc attctcggaa actggaaagc ctaaatcgta tttaagtgag aaaaataaat
    44581 tctttattta tacagaaaga cctgtttatc gagtaggtga ttctatattt tttaaaatca
    44641 ttggtaaaaa aagcgacggt aagtttttac ccgatgcaag agtaaaatta aattataaaa
    44701 ttacaagtcc cgatggcgag acaattgaaa caaaggatat tgagctagat gattggggga
    44761 cttatagcgg cgagtttatt tctaaaaacc aagatgctct cggtgagtat ggaattaatg
    44821 ttagcaagga caaatcagag ttatccgctg ggtcagctta tttttatata gaacaatatc
    44881 gaaagccaga atacaaagta gaagtaacac cggcggaaaa tgcctatgta aatggagaga
    44941 cagcagagtt taaagtagag gcaaagtatt tttttggagc accacttgcg aatgcaaata
    45001 tgagctttcg aatctatgaa aataaactaa gagatacgaa tacaacttac tggtgggaag
    45061 aagaatatga agcaaataca acatacaatc agattcgaat ggagggagat aaggaactaa
    45121 atgaaaacgg tcttgctgtt ttaaaatata aactaggaaa ttacccctat gatagagaaa
    45181 taactttaga agcaactgta accgataagt ctaacgtttc tatcacttct agaaaaacaa
    45241 ttactgtggg aagaggaaag ttttatatta agatcaatcc agtaaatagc ttcttttata
    45301 caaaggataa aaaagaattt gaaataaaaa ctcttgatta tgaaggaaag cctgtcagtg
    45361 caaatgtaac tttacaattt ttccctttta tttggaagcc ttggcagaga gtctatatgc
    45421 atgcagataa gcctgtcttt attcaaaaaa tcagcaccga tgagaaagga atttataagc
    45481 tagaattgaa taatgaatta gcgactgcag gagaatttga tattgttgct acttctcaag
    45541 acagcatgca aaatcaaatc acagcatctc gtgttgtttg gctttattca ggaactaacg
    45601 atcgattgga gtcaaagttt aagaatattg aattgacatt agataagaat gcaattgata
    45661 aacctggaga aatcacagtt ttactaaaaa gtaaatttgt agataatttt gtttgtgtta
    45721 ctttagaagg aagagatatc tatgacagaa aagtagtaaa gatggaagga aatttacagg
    45781 cagtcaaatt aaatataaaa cctgaatatg ctccaaatct atttatcact gctaccatgc
    45841 aaaaaaatag agcgttatac actacaagtc aaagtcttga atttcctcat gtagatactc
    45901 gtgtgatgat aaaagtaaaa acagacaaag aaaaatattt acctagagag accataaaag
    45961 tagaaataac agctcaaaat gaggaaggaa aacctataga gggagatttt tcccttggtg
    46021 cagtggatga agcaatctat caaattcgtc cagatcatac gagagcaatg aatccatttt
    46081 tctattcaaa aatttcaaat tgggtttcta caaattattc ctatccgatg acacttctag
    46141 cagggactac aaaagatggt aaagatttga ttcgggaagt ttttaaagac actgcgtttt
    46201 ggaagccaga tttaagaaca gataaaaatg gaaaggcgtt tattcaattt gaagtgccag
    46261 ataatttaac agaatggaga ctaacgctta gaggtcatga taaagaaggt agggttggag
    46321 agactgtaag ttcagttcta gtgactcagg atttggttgc tcgaatcgca aagcctcgtt
    46381 tttttactga atcagaccgt atcagtgtga ttggaattgt aaataataat tcgaaagagg
    46441 gaatgcccga gattaaaact gaaatgctag ttaatgagaa agagataaag ccagaggaga
    46501 aagacaaaat aagtctacct ccttttggag catctaaaca acattatccg cttgtagttc
    46561 ccgaaacgga ttctatgaaa ttgcagttta gagcaagtgc aaatgaaaaa gcaaaggatg
    46621 cgattttaca taaagttcca atttataaga atggaattgc atttaaaatt tcagagcagg
    46681 gtgatatgac agagaacaca gttgttcagt taaggcctgc aaaacctgac aaaaatattt
    46741 ctgctcatgc agaggaacta tcgatttctt tgaatcctag tccagtagag aaaatctgtg
    46801 cagcaagaga ctttttagca gattatcctt atggatgtgt cgaacaaact ttgaatcgat
    46861 ttttgcctgc tgtggaaatt tggaatctta accaagctaa aaaacttcct tccataaaag
    46921 atgatccaaa tctagaaaag aaagtaatag aggggatcaa acgactatct gaaatgcaaa
    46981 acgatgatgg aagttggggt tggtggagtg gtgatgttgg caatgaattt ttaactgcct
    47041 atgttatgta tgggatgaag cgtgtatcgg ttttacacat cgatcaaaat gcagttcaaa
    47101 aaggtaagac tgcattgact agaatgctct ctaatccgca gagtatggag gatgatgcaa
    47161 gagcttatct attatttgta ggaagtcttt acgatatttg gagtccttct tcttatgaaa
    47221 aattgataaa gaaggatgac gttagtgctt atgcaaaatc atttttaatt cgagcacttg
    47281 taaatctatc tccaaaagca tttacaaaag atggagaaaa aattgtaagt gaactaaaga
    47341 agcaaattcc aattcttgta gagagactga aatcctctca taagaatgac aaaagaggaa
    47401 tctattatga tcattcagag gaaacgtatt atagttggca gggtggttct acagaagtat
    47461 cggctcatgt gctatctgcc ttgttagaag cagacgataa gtctgctata actgccaatc
    47521 ttatgagttc aattcaaaaa agaggaagtg gtggatactg gaattctaca aaggagacag
    47581 cgactgtaat ttctgccatt acatcttatc tagataaaaa aggagcgaat ttctcttcga
    47641 aaggaaaagt ggatttttca ctcaatggga aacctcttac ttctatagaa tatgatacga
    47701 gtaagagtga tcgggagttg aagaaaatga ttccaataaa ggaatctaaa gatgaatata
    47761 caatagccgc aacaggaact aagtcttctg atttaagttt tgatttatct ttgaaaggat
    47821 ttttgaaatt taaagacaat gatacaaata gtcaggctac gcagacaagt atagactcat
    47881 tgcgaaatgg attaaaagtt aaaagaggat tttattcgat caaaagaatt cgggatttga
    47941 ataaccaaga atattttgtt ccacatccat tagtgcaacg taaattgcaa gtcggggaag
    48001 agcttttagt aaaagtccaa tttaaagcgg ataaggatta tgagtatctc atgttagagg
    48061 actttttacc ttctggattt gaagtaacaa agacagatgc ttatgaggga agagtttatt
    48121 atacacattc agaaagaaga gatgaaaaaa tggttttctt ttttaataaa ttaaagaagg
    48181 gcaaggttta tgaattggct tatatgattc gtgctgagtt gcctggtgtg ttcaatgtaa
    48241 aaccagctcg aatggaatgt atgtatgcac ccgaaataca aggttttagt cttcctgaaa
    48301 aactcactgt agaaaaatga cgttgtgcag tatagcccaa gttgccgaca ggtgattctg
    48361 tcaaattaag gattttcagg caggaagggc acgaagactc acgaagtgaa taaagaataa
    48421 gccttctctt ttcttcgtgt tctctgtgct ctttctggtt aattcttttt catttctctt
    48481 cacttgacag cattagccga caggatccct gtctcctatc ttacgcgctc gtgcgtaaga
    48541 tgagtagcat gcgattcttg cgtaaaggtt aaattaaaaa aacgtgaatt cgaagtaaaa
    48601 aattttttaa cacggatgaa caccgataag ggaacgataa agacacgatg gataatttag
    48661 aattatcgta tcttttttcg gtgatcatct tttgattttt ctgttacctc gaactgatat
    48721 gaaaaaaact aaaattattt acattctcct tcttttaaac aaataatttc aatgtagaag
    48781 agtgtttgaa atttactatt cgaataaaat taaacctatt tattcaaccg atgaattgtg
    48841 tttagattgt gaataatgat ctaaaggaat gcttgcaaca tcaattatgt tttcttttga
    48901 tttagttcat agagtttgat gtatttaaga aagttgtaat acgatccaag aattgcgatg
    48961 atgagcccaa ctttcccatc taaaaaccca aatcgaataa tatacattga aataaatttc
    49021 caaatcgctt ctgcgatggc aagacttaaa cctgacttct ttcctttctt gaatttttct
    49081 acagcagcta attcagagta tctatctata aattttaaat gatcagaaat atttcgatag
    49141 gagtaatggg agagaggatt cttgaaatat tctactttcc cttgaatcga tacagtttca
    49201 tgaactaaga ttccagagaa ttcaccttta tctttttgaa aaaatcgcat ttgataattg
    49261 ggataccaac ctccatgacg aatccatttt cccatataaa gagtcattct aggaataaaa
    49321 aaaccaacta cttcattcat tttttctttc gatacagaaa gaatttcttt ttttaaatca
    49381 agggagattt cctcatcagc gtcaagcgct aatacccatt gatttttcgc caagctttta
    49441 caataattct tttgattaat ataagtatca aattttctat aataaagaga agttttagga
    49501 tagtcacgag cgattactgt agtgttatcc gttgatcctg aatcaacaat gataatctcg
    49561 tctgcaaagt ctagacttgc gagacaacgt ccaatattat cttcctcgtt taaggtgatg
    49621 atgcaaactg aaataggaag tgtcattcaa ataattttaa tttcgggctg atttatattc
    49681 attcgtaaaa ggaatctcta atcttgctga tgtgtattca tttgtaatcg atatgcgaaa
    49741 gtaattggga tctattcctt ctcctttcat catgataatg atgagtgcaa gaccaagtcc
    49801 tgcaccttct gtattatcag cattatccat atagaattgg gcaaggtcgt catacgacat
    49861 agctgttgct agtttttcgc gaattgattt ctcttcttgt tcagcaatga gagtgttatt
    49921 tctaatttct atctttaggc cacttgcatc gtaatcaaaa atgattaaac aatagtaatc
    49981 ttttgctttg catttgggtg catatgcttg tcccatattt tcactgaata tttttttata
    50041 ctctttgatg cctatcgcat aatgttcggg attttttata tcaaaatgat tctcttcaaa
    50101 gaacacacgc ttttgatttg ccttggttgc attaataatt aactctttta taacagtgta
    50161 aagaattgga attagacgct ctttttcgaa ttttgttaaa ataccttgca gtgtttcctg
    50221 cactctttct tctaaatgcc tggatgtcct gtaggtttta atctggattt catttgcatt
    50281 ttgctgtatg aattccatat catactgaga atctaactgg tttatttgca taatttaata
    50341 tatatatcgt ttgttatctt tgtcaaaatt tttatttttt tgttaaaaaa aagggaaaaa
    50401 aagtataaaa agagtttga
//
LOCUS       DAOQOS010000027        50226 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_262_length_50226_cov_6.484344, whole genome shotgun sequence.
ACCESSION   DAOQOS010000027 DAOQOS010000000
VERSION     DAOQOS010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 50226)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 50226)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50226
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_262_length_50226_cov_6.484344"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..345)
                     /locus_tag="PLF66_07905"
     CDS             complement(<1..345)
                     /locus_tag="PLF66_07905"
                     /inference="COORDINATES: protein motif:HMM:NF023866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="von Willebrand factor type A domain-containing
                     protein"
                     /protein_id="HMZ64001.1"
                     /translation="MKIIKYLAILSSLSLIYCSASEDKTYSIAQSEPAREMNKPTASY
                     KNKQQSTKIYTKDQKPSSPKTKPEMNTASGVSDSEEYNTEQYDKITDNPFLDVKENPL
                     STFSIDVDTASYS"
     gene            complement(342..1313)
                     /gene="meaB"
                     /locus_tag="PLF66_07910"
     CDS             complement(342..1313)
                     /gene="meaB"
                     /locus_tag="PLF66_07910"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF006958.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HMZ64002.1"
                     /translation="MKYTDELIKGILEGNRRALAKSITLVESSRFEDAKLSDEVLSGV
                     AGHSTKSIRIGISGVPGAGKSTFIEAFGMHVISQGKKVAVLAVDPTSPITGGSILGDK
                     TRMPELTQSENAFIRPSPTSGNLGGVARKTKDTILLCEAAGYEVIIVETVGVGQSEVA
                     VFDMTDIFLLLLLAGAGDELQGIKKGIMEMADIILINKADGSNTINAENAKHEFENAL
                     RLFKPKNSFWKSPVLTVSSLLKTGIPTVWETIQNFIDASGEQMSINRKSQYDKWLWSY
                     LQNQIYYELQTLKEKNPKIKKIEEDVRDNRLSIRTATHTILEEYKKS"
     gene            1433..1708
                     /locus_tag="PLF66_07915"
     CDS             1433..1708
                     /locus_tag="PLF66_07915"
                     /inference="COORDINATES: protein motif:HMM:NF014643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HMZ64003.1"
                     /translation="MNNYFNVAQAKSRFSEVLHRAEISPQFISSRGKDVGVVLSKKSY
                     EKLLQIENDNLPKTKLKNFLALSKSLRSNFPQKLPSSARKSRQAPQF"
     gene            1721..2125
                     /locus_tag="PLF66_07920"
     CDS             1721..2125
                     /locus_tag="PLF66_07920"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HMZ64004.1"
                     /translation="MSNYLLDTNVISELARKSPNPSVLDFFETIDDITMSVVTLEEIE
                     FGIERLPASQRKTLLKWWSALLSLPPIIIPVEEKIAKLAGQLRARQESKGRSKSQADM
                     LIAATAISNGLILVTRNTKDFYELGVSVLNPF"
     gene            2135..2563
                     /locus_tag="PLF66_07925"
     CDS             2135..2563
                     /locus_tag="PLF66_07925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64005.1"
                     /translation="MIQRAIEIALDAHRDQTDKNGQPYILHVMRVGLRGRTDEEKILG
                     ILHDVVEDSSWTFEMLAKEGFSERILSALKCLTKKSEDEPYEEFIGRVKENPLAISVK
                     LNDLEDNMDVRRMEIVQEKDSKRLNKYLKAYKELAELKNQ"
     gene            complement(2557..3090)
                     /locus_tag="PLF66_07930"
     CDS             complement(2557..3090)
                     /locus_tag="PLF66_07930"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004425963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HMZ64006.1"
                     /translation="MSIVKSKIRTIPDWPKPGIMFRDITSLILDPEGLALTIGTFVAR
                     YEKQGITKVAAIEARGFITGAALAFQLGVGLVPIRKKGKLPGETIKQEYNLEYGTDIV
                     EIHKDSIVPGDKVLIMDDLIATGGTLGASIQLVRALGGEVYEAGVIVDLPDLNGSKII
                     KEKYGVNVYAICEFEGH"
     gene            3289..4638
                     /locus_tag="PLF66_07935"
     CDS             3289..4638
                     /locus_tag="PLF66_07935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64007.1"
                     /translation="MSEEKLWYSKKNAANCSQCLAPDSIEIYYYQPTRQPVITGDEFV
                     ITYQVIQKTKKVCVNCNFTEEFFEEWKEYKYPKKEDIDFLKRRAIYFQTNFKYKAEEA
                     FADYLERNPSDFEMLFEFGRYLLANREIIKAYSNFKKSIEKNPNFLPAMEAITDLLFQ
                     LLNAQNDYWFECLPYYGFYSHIHLTYQNFLNELNKYLRLLIEHHKNNLQLLPAYYLKT
                     APLLEEKFLFPLALEYYEYVMSQSPFKVEQADASLRAGLLYAQLQDYPKAISLLDNSN
                     LLLPNLMIHYHKAKVYRKTEDSMKSNAEVSIFLKSLDSLILKNENDPRLYYFKGLGLE
                     LIQDSKELIEYYQSVIEKGKFDIPIRTIFQRKINNLLDPPRREIISSQNFNCPVCKSN
                     EFIFYTYRDQVVGSIKPKESLNTVYYYLQVLRKICNHCQFEFKEYKSWEDIDFDTQI"
     gene            4687..5436
                     /locus_tag="PLF66_07940"
     CDS             4687..5436
                     /locus_tag="PLF66_07940"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HMZ64008.1"
                     /translation="MNAMIITGASSGIGRHLAFEYASRGFALGLTARRFELLQKLRNE
                     IYEKFGSNIKVEIRTLDVTDYKKIYSVLKDLAHSLGNITHVVANAGLAGSSPIGSGKF
                     LHDKFVIETNLIGAMATVDAAAEIFKSQKSPGHIIGISSIAGFRGFPGNASYCASKAG
                     FTVFLESARRDLKDFNILVSTIHPGFIATDINAHIPSKPFVVSVEKGASELAKLIDQK
                     VIVSSVPKFPWGLIGFLLKWIPNVIWQRLKF"
     gene            5433..5948
                     /locus_tag="PLF66_07945"
     CDS             5433..5948
                     /locus_tag="PLF66_07945"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002103739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HMZ64009.1"
                     /translation="MSNKIKVLFVCLGNICRSPAAEGAFKSLVEKKGISELFEIDSAG
                     TAGYHVGERPHETTTKVARDRGIILNHYCRQFEPMDFQRFDYILAMDQSNFRSILSMA
                     KTESDKQRVMKFRRFDSTVKGEPDVPDPYYGGIAGFEHVQDIVERASEGFLNWLLENH
                     SLPTELEENED"
     gene            5953..7203
                     /locus_tag="PLF66_07950"
     CDS             5953..7203
                     /locus_tag="PLF66_07950"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017858295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HMZ64010.1"
                     /translation="MVEEKHIVIVGAGFGGLQAAKKLGNKKGIKVTVVDKNNHHLFQP
                     LLYQVASSVLSPADIAIPTRSITTSYANISIIMSELKEINKEHRKLHLEHTTIQYDYL
                     ILAMGARTSYFGNDHWKKFAPGLKNLNDALKVRKSILVSFERAELETNPAKVAELLNY
                     VVIGGGPTGVELSGAIAELSHQIIRNDFRFIDSSMSKITLIEGGPRLLPSFSEKSSDV
                     AYKELVKRGVDIRLNTRVVNIDEEGVHTGKEVIKSQNIIWAAGVAANPFTQKLDTPLD
                     KSGRVIVNSHCAIPSHPEIFCIGDMANFSEGLERPLPGVSPVAMQQGRYVADLIMDEI
                     KGKSRKPFHYFDKGSMATIGRMDAVAEVGNIKLSGIIGWLGWLFVHLFYQVGFKNKVS
                     ILITWIWSYISFKAGARLIQNEED"
     gene            complement(7200..7580)
                     /locus_tag="PLF66_07955"
     CDS             complement(7200..7580)
                     /locus_tag="PLF66_07955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64011.1"
                     /translation="MSEYLGQILNFHCVEQNSFEVKILIDTKDNFHKIMSYYRGENLS
                     DYTTYNINIIYSENDEILMTPDENANECMAIYADNLLEDVLGTESESLWQKGSIIDNL
                     VYDLINSSLLRPLSTKDIEIEEDD"
     gene            complement(7628..8566)
                     /locus_tag="PLF66_07960"
     CDS             complement(7628..8566)
                     /locus_tag="PLF66_07960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64012.1"
                     /translation="MRKIVFTLLFILILSSNYAETILLKDGEVIQGKIRNQNINSIKV
                     EVNGNGELREILKEKIYKIIYRDPNQSVEDKSKSEEPILPSNQLEEDKKKRQDESSDA
                     KDKAPLLKEEEPKPITIEADPPEEKQNVNLEPSKGEEPKRSIPSRLAVTGCSIILPGC
                     GQFMERRYMAGILYPLLLIAGGAAYSSEKQKHANAVNNFNSLSNPILLGGFLLNFSNP
                     PPPIENTFLIYYAQKSSVENSHVSSARNIGIGIALIYLFNIFDAYYFYNENAFTDNRT
                     RGFFLDYQLVSQSGITSVSHISKYDPYYIAGYSFRF"
     gene            complement(8838..12896)
                     /locus_tag="PLF66_07965"
     CDS             complement(8838..12896)
                     /locus_tag="PLF66_07965"
                     /inference="COORDINATES: protein motif:HMM:NF024487.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HMZ64013.1"
                     /translation="MNYSQTLLDKACEQWIQDLTDLSRRNNLLYYKDQKVGTLDISKL
                     DEKYLIEFFGGSKVSINDLLSLKDNNADVYRKIKIINKRAKQNLEERGIDTLLLAYGF
                     ATWKEDDDASPPRCPLILLPISIESKSKAFLRRSGDAFINPVLLAYLEKRHNLKITSD
                     QLLQDENGSLNEDSVPDFETVVSRLRTLSAKRIQSIQVDKQFIISNFSFQKMSMVEDL
                     KFQKENPNNQTELVLAMAGDEFAIRKIKTSTVEIDPKKFDQIQPEDDFIVFDCDSSQQ
                     SVIQAVLKGENCTIQGPPGTGKSQTIANLIATLVANGKKVLFVAEKRAALEVVKRRLD
                     QKNMGHLLLDLHGTGISRKMVLDQFKESLDLIKSFRSIDVGETHKQYVKKRRELNYHV
                     EQFHKKRSPANKSVYELLSALIQFSEAEKSQTKFTGKDLEKLTPDVVDSLRAHLEELQ
                     KPSLASLFVNPRASIWQNTNISTVEDGTELSNSVNTIYKQLLFLEQEFSKLGAYGLSD
                     NKNFKEMENMIQLMERVNLMYTLYSDNHDIFHLDTKNFLTIKKAFANDTDLNQGFDYL
                     KQIHRTPDTTPLLVNEKINLLTDISRISDKYSDEIYNIDFDKYIQILSNAESKKWFSN
                     LFDSELKTTVETLKKIRFSEEVSREELLGEIKKISVNLNKWKKLFPQYEFPDMKIIKL
                     LRDNEFFLKVANSDFWKAYESIFNSRNPIAETSSVFFTDIENIANLLKEWKSQHRSTI
                     YPEPVRDWETISSNFAQLQQEIQKIKSRINQNQIEKLGIGEIISIFKELNNDSQALSL
                     GSVKEIEREFNKHNASVFLIEIKQRGIESEFWARQFEYAWLYSCLEEAYKQDGKLKTF
                     HGKTQTELVKEFQELDRERIELTKDRLNYRHARWVMQQMSQFGDEENLVRGETNKNSA
                     RLSLRKLLPETPNVLTSLRPCWMASPLSISQLIDAKKQYFDVVIFDEASQIIPADSIP
                     AIMRSKQIVVAGDTKQLPPTDFFRESDDDDDESEEVSHTKGFESLLNRTASFLNERHL
                     VWHYRSRSEELIAFSNENIYANKLITFPSPKNQRVLFHDLIPFEIGQTPEESEEMEVK
                     RVIDLILNHATERPNETLGVITMGIAHAQRIESALEDALKSSMDLEPFFNEEIEDHFF
                     IKNIEQVQGDERDAIILSIGYVKEANGNLPHNFGPLTHQGGERRLNVAITRARKRMTI
                     VSSFRDSDIDLDRSNSEGVRLLKSYLNYAGYGGKPQASYGQEKLNPIEKDIMETLEKN
                     GYTVIPKFGFSQYKIDLVVRHPKKDEFVLAIECDGEYYASSPTVKERDRLREQQLASL
                     GWKFYRVWLLDWINEKESELERIHQAFQEALK"
     gene            complement(12908..14386)
                     /locus_tag="PLF66_07970"
     CDS             complement(12908..14386)
                     /locus_tag="PLF66_07970"
                     /inference="COORDINATES: protein motif:HMM:NF036494.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HMZ64014.1"
                     /translation="MKKAIFLFLFLFGYSSLFSLETIEDKFKNSIVQVKITTQSPNFL
                     FPWQTKKPQTTGAVGIVIANKRIIVFTSLIDYYTSIEVKKFSSYTSFPAKVIKMDYES
                     NLSVLEVSDKDFFNDLVPIQFESKIDPFKAVSILQLDNSGSIQNAKGRLIGIEMENYP
                     MGYTELPYLNINSNEKLDGDGELILDKDKAIGVLFRFTASKNSGKAIPGFLINTFIKQ
                     KFDKPTGTIFPFKGFRFRPLQDEATKEYFGLDKSMQGVLVAEILPYSTADNSLQLNDV
                     ILEYGGMKIDSQGYFKHPIYGKQYISFLAHCGEEFGFKMGKNIPVKILRNKKEETISM
                     RLKPFPYKAIKIPYMNNEGKEPEYLIRGGFIFVELSEFLMKEFGSSWRSRIDKKLLYL
                     NDYYRYHKKGEQGRYVMLVQVLPDDSNNGYHSLSFDIIKTVNESSLNSIRDLDKAISS
                     DKERYVSLLTENGTEIVLDKTTLKEIDERIQKKFGIERLKNF"
     gene            complement(14383..15843)
                     /locus_tag="PLF66_07975"
     CDS             complement(14383..15843)
                     /locus_tag="PLF66_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016545644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HMZ64015.1"
                     /translation="MKYILKLLLILLPLNSIFAEQISDFDKVRKGVVQIRTYSQGFDA
                     FSPWLTTGVRTSGGTGFIIDKNKIMTNAHVISNAKYIQVQRYNQTTWYEVEILFVAHD
                     CDLALLKAKDDSFYEDSTSFELGAIPDLNSSITVVGYPIGGDKISVSRGIVSRKEQST
                     YAHSEVDSHLVIQVDAAINPGNSGGPAIQDNKVVGVAFQVASRGENIGYLIPTTVIRY
                     FLNDIKDGKYDGYVELGVRTLNSFNNTLREFKKIPKDLNGVFVTKVLKGGSAEGYLKK
                     NDLLLEIDGLPIGRNGTIVLDKDARIDFVEIVDNKHSGETISFRVFRDGQMMTVNFPA
                     KRMDEFTYMRHKYDEPFDYFVLGGLVFQPISRDLLQEWNKNNETQGGSQFLYRFFYFI
                     DDDLCKEVDSDVVLYRKLAHPINTNSDYFLNMIVESVNDTKIQNIKHFKEVVAKKDYS
                     FLKIKFRDIDIPLILKKEDIEKADREVQAIYKVGNK"
     gene            15979..17121
                     /locus_tag="PLF66_07980"
     CDS             15979..17121
                     /locus_tag="PLF66_07980"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HMZ64016.1"
                     /translation="MNLIVEIIFSSLTILIVSILYLVSVFRVPITSTKITFGIFGFLL
                     SLWNLFEILQKLQLVQIDSYYLQEIIFSNLTIYALVIFALHFPFYRRREVMITVYTAF
                     VGGVGYLITIQTISIPYVSQYFPLESRFYESINFFLTRAFSIFAALSFLVIIIYKLTH
                     ASKGLRKFFYRALSYSIILTIFILTLNYILTLDYKVLKANINVYLIDLLFLSIVILSL
                     IQFKFIAFYPGFLSIIIYRELPRLVIQGIAPSNLDGAQSLKEELWKIYEVENWKAFLN
                     EFWFGIIIDETLDNAVEHGGRRSDDEITVQVFETSRFLDIYVIDSGKGFDPELVPNPA
                     SPERINIPTGRGIHIMKKLFHVTWNFLGNEIRVRVSKNPEDNPEEA"
     gene            17229..17507
                     /locus_tag="PLF66_07985"
     CDS             17229..17507
                     /locus_tag="PLF66_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010409878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04454 family lipoprotein"
                     /protein_id="HMZ64017.1"
                     /translation="MKKLLLVAMLLATSIAFSNCSKPAVSAAECEPVVNQMFENLAKE
                     LKPEEAEKVKAMEATLKAGVVKECTSGKYNLDCLKSATNIAALQTCKK"
     gene            complement(17744..17980)
                     /locus_tag="PLF66_07990"
                     /pseudo
     CDS             complement(17744..17980)
                     /locus_tag="PLF66_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491582.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(18188..20209)
                     /locus_tag="PLF66_07995"
     CDS             complement(18188..20209)
                     /locus_tag="PLF66_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010572250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein meaA"
                     /protein_id="HMZ64018.1"
                     /translation="MENKKAHHLYDENGNITKDKPWIFRTYAGHTNARASNELYRKNL
                     AKGQTGLSIAFDLPTQCGYSSDHPIAKPEIGKVGVPINSLDDFKVLFDQIPIGEMNTS
                     MTINGTSMWLLSLYMALADETGVPYSKLEGTTQNDIIKEYLARGTYIFPPEQSIRIIV
                     DMYEYSLRASPKWNPANICSYHLQEAGATPVQELAFALITAMAILDAVKERNCFNEEE
                     FEKCVGRISFFVNAGIRFIEELCKMRAFSEMWDEITRTRYNVKNPKYRIFRYGVQVNS
                     LGLTEEQPENNAWRILTETLGVLLSKNARCRALQLPAWNEALSLPRPWDQQWSLRLQQ
                     ILAYETDLLEYPDIFDGSKVIDSKVKSLKEEAYAEIEKILAMGGAIKAIENGYMKSQL
                     VKSQSERMAKINSGEITIVGKNKWTDGIKSPLMTDTDGGIFKVDPASAQLTLNSLSET
                     KTKRDPERAKKALEALEHAARNNINMMPASIECAKAGVTTGEWADTLRKVFGQYQPAT
                     GVEGQKLKIESDLVNVIRTKVDEFMKTTGHRPRIVVGKPGLDGHSNGAEMIAIAARHA
                     GFDVIYSGIRLTPQEIVQSAVEENADVIGISILSGSHKEIAEQIKEELIHYKAFEHIP
                     VIFGGIIPESDFDALRAIGIKDIFTPKDFDLMQIMDKIIDLISKHKKAA"
     gene            20283..21599
                     /locus_tag="PLF66_08000"
     CDS             20283..21599
                     /locus_tag="PLF66_08000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64019.1"
                     /translation="MLVYRENEPPVNYMGFLLSLIFLFISLFIFYSFKGEPKNQDSFL
                     RLANLKSKVETGVIRTYTEPVTPFLIFIWKKVTGLSYSNAYQTFICFTLSLFLHMIML
                     VYRKNEWRLNHYFLVYLVAFLPFTIYLPLEYLEEMVCLIVILLLQVDFKMENPGDLFK
                     LITMTILAFMTHLIMFMVGYTVFAILNAIDFMNRQKAKPTVFYKKKNVAGQLLLVYLI
                     TLIVFVIIASWFKFFGLDSFHFLWTNFWDSFFKFFPIFVVLKILEYLLRKEKELNTLG
                     TTGIVILLIVVSGYFMYKAKNENIDYLETQKNELLSLKARGKILQGQAIYGYPLISDY
                     MYFYAGETVLTAEVSRMKKDDFLITRGMVVSDKQILERSIRSRNPLFFPLDKDTLLIG
                     KELIDFIMLEKANTETKTKLFETLAQIPPITPYTRMNKAFSSLFGL"
     gene            21596..22216
                     /locus_tag="PLF66_08005"
     CDS             21596..22216
                     /locus_tag="PLF66_08005"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025033.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HMZ64020.1"
                     /translation="MSFEKNYWSEVYSGRLIDGTFNAAKHADYIKSIFSLSEFRIRKI
                     IDIGFGKGKLLEEVSKKLEPESLIALDPSKLMVSSLLKKHWISKYNIAIVNKKLEEFN
                     IDYLQKYPFDLAIANSVFQYIEDLEGQVKRLASIARFSYFSVPTDKDYVRMEEEFNFK
                     DKYAFARSKKTYLDCISKYFTRISFNLLESKIVKEHSYFQAELYRE"
     gene            complement(22252..23190)
                     /locus_tag="PLF66_08010"
     CDS             complement(22252..23190)
                     /locus_tag="PLF66_08010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64021.1"
                     /translation="MKPNIKLIAKILFIGIAFVFTIKWLGIEKRVFLSSDLVCPSFGN
                     LSTIAIPKQSIYIDPIHSIKKIGIRNKLGEKKFYPASFLNGALFIKSQNELNEYLSIY
                     ADICGGKPPFYQSIDFVKIARIKNIENQRLENTKESRKDWRNFYAKEKRLISDKSFLE
                     NKIASLQSKLDRITPVSFNIIGKVAETTKDSLIVFGSAVPDSAKAKDNMGFVYNGVII
                     LKNAKKSDIASPDYYSTKHVLAKDYYFVKRIDGRDTFGHFMPVSVYARNVKVTSPKVL
                     SELRKEIANYESKLSKIELELKSMEHIEEHYLATVM"
     gene            23558..23860
                     /locus_tag="PLF66_08015"
     CDS             23558..23860
                     /locus_tag="PLF66_08015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64022.1"
                     /translation="MKRRNLTQQPGFGNFKNKCSDLVRRYDTRIVRMIYEVVYENVYW
                     KSGTKDSIQKEILAEVYSKLKEQRISILHLQMIVSEMYEYFGKFLVLLDFGKRRQN"
     gene            complement(23857..24651)
                     /locus_tag="PLF66_08020"
     CDS             complement(23857..24651)
                     /locus_tag="PLF66_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004440636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HMZ64023.1"
                     /translation="MKIKFWQNKNIVITGTSSGIGESLLMELKDIPCRIFALDRSPKK
                     IEFFHNAEIIPIQCDISLDSDIIEAQKQILSKTDTVDVLFNNAGITTHGRFDETDISV
                     FRKTFDINFFGTVHLTSKLIESIKKAKGVIVVTSTVSGLYGVPGRAAYSSSKSALHAV
                     FESIRIELSEFGVRSILFCPPYTKTNLRTSGLTGAGEKLNDKQYKGNLLTPNQVAKAM
                     IDAAEDPNARLVTMDPKGFFVKILRLFAPALLEKQMFKKLYADFHH"
     gene            complement(24719..26524)
                     /locus_tag="PLF66_08025"
     CDS             complement(24719..26524)
                     /locus_tag="PLF66_08025"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HMZ64024.1"
                     /translation="MKLSSKINQYILIKDLCSFLAFGIGVVAFIGWILNSKLLLSLNP
                     SFKPMPPNGALASVLLGICLFFYDSSFHWVKKFLTIASIFLILISFFRMFEFVTEIDL
                     GVDYLIFHFKNYGKWYNYAARTSFFGALCIFLGSIGILLLSANQRYKLSEYVSLFSGN
                     IVAFIGTIFSLGYVYGVPLFYSHNLIPMSLNSAVSFCFVGIGISLCSLIPELKENLKT
                     EVQLDEAKKQIESAFHYSASGMALIGINDSWIRVNEPFGNILGYTKDELISRQFYSLL
                     EKDVVIDYILKFDDLLSGKVESLQMESKLEHKSGALVWALLSISLLRDKNNLPEYYII
                     QMQDITRLKKVETDLIHAKNILEDALQAKSHFFAAMSHELKTPLQSILGYSELIEEEA
                     NQLNATQIIDDSRKIHKSGKSLLKLINDILNIAKLDASKVEVNYHLFDLKTLLEELID
                     TIKPLLVANHNRFDLSVAEEIQYIYQDMEKLKHILLNLLSNACKFTTEGKISLSVQTK
                     DIDSKKWLQIEVEDTGIGIPEEDLTRIFNPFEQSFAENTRKYSGTGLGLTIAKQFANL
                     LGGDLSAESKLKEGSRFIVMLPLEESVNEGILDTI"
     gene            complement(26537..27295)
                     /locus_tag="PLF66_08030"
     CDS             complement(26537..27295)
                     /locus_tag="PLF66_08030"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HMZ64025.1"
                     /translation="MEPKQKDKYRTYRILLLEDDANSAEIVTRSLEKYNIHIDHVANA
                     RLAVSKLKNKYDLIISDVMMPIMDGFTFIEKHREEIQNTPIIILTALQEKEDILKAAA
                     LRITHYLVKPFEQAKLVNKVIEAMGIDPSFLILKKDYPFSISHDVIDESSIKLIIKGF
                     ATPSQFRTALPNYIMQLKTYSNNIKDFTIFVTGDFNYTSSAIELLDGMIQELIDSFST
                     RENHILLKGDFFQKVAPEDIVKTKWIRVLLSQNH"
     gene            complement(27298..28746)
                     /locus_tag="PLF66_08035"
     CDS             complement(27298..28746)
                     /locus_tag="PLF66_08035"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HMZ64026.1"
                     /translation="MRYKLWINGDWEETGDTKVISSPYTQTTVALCEQANEKLIDKAI
                     LSAYETFPFFKKSSRYARSLLLKYIAAGIESRKKEFIETIVNEAGKPIQFAEGEVNRG
                     ITSFQIASEEAKRYNGEMISLDGDKSGVGFDPAFSYFVPKGVVLAITPFNFPLNLVAH
                     KVAPALAIGATVILKPSPEAPGAANLLTEVFLESVKLVSNEFEKIPSTALQLVHCSND
                     LASKMVKDKRVAVVSFTGSDKVGWELQKLAAGKKISLELGGNAAVIVHKDADIDLAVR
                     RCVLGAFSYAGQICISVQRIFIHHDIYKEFKRKILRETEKMIAGDPFQKETSIGPLIN
                     KKARDRIFAWVEEAKEEGAVFLNSVNAVDNIIYPIVLESVPTHIKVYESEVFGPIVML
                     DSYFDFAEAIERVNESRFGLQTGVFTDSDKIMRQATEEIESGTIIFNEVPTFRVDHMP
                     YGGIKESGLGREGIYFAMEEMSERKTIIKKRI"
     gene            complement(28794..29576)
                     /locus_tag="PLF66_08040"
     CDS             complement(28794..29576)
                     /locus_tag="PLF66_08040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64027.1"
                     /translation="MVLLINFQKRNESKSNSIEMKFRNRWVFCISCLIFIYCQNHSER
                     NPKKKDLILFAALYTYQKTQSTDCKDQYKYLNSLYATYASATFSSYSTQCDAIILGDS
                     TMDLARATSFYDATKTLNYAISGNTACDYFYQIENIRCNPKNVLIATGDGNGVLRKVS
                     SANSIETMRKVIAKIKGKWNANIVVIGIHPILLPSENSLKNPVNQGVSQLGVCYINPL
                     PIFGVGENDPPDSSLMIDSIHYNPTIYPKYKTQITSQCGVSF"
     gene            complement(29542..30681)
                     /locus_tag="PLF66_08045"
     CDS             complement(29542..30681)
                     /locus_tag="PLF66_08045"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HMZ64028.1"
                     /translation="MIQTTNLYIEFFGLILLLILLVSFQSFRNLFTFSNSPPEKKGER
                     SRVVDFVRGVAMCAIVVIHVDSYFTFFHPKDSELILSKLMANFSRFCVPAFIFSSGFF
                     LSWKGFSAFWNSKIKNLLIPYLIIACIGFFTKYPAESFLSDIIPKLLLGQVFQPYYYV
                     PLLFSFYLLYSLFFRNIQNWSANLFYLVLILSLLINVWSNHSYPKSLPYIGGMEAISF
                     TNYLFFFVSGFSAKRLLTNKNLFLDSLSANRYYFQGLVITILIYLSVVTYYTVDLNLE
                     ISNHFLFYPIACFIVFTLIGIRLESTNQTFLKKIYSVFVFIGENSLALFLLHPMTIHL
                     MHMLDPYYMGGFYLGWIFTFAINIILPLLVWKMAYGIIDKFSEKK"
     gene            30790..31179
                     /locus_tag="PLF66_08050"
     CDS             30790..31179
                     /locus_tag="PLF66_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008589074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HMZ64029.1"
                     /translation="MASIVSLKAGKTIFKEGDFGKSMYIVLEGSVEIYVVLNKQETHL
                     ASMNQGDFFGEMALFRDKPRSACARVISDVRLAVIESRAQLEKFLSDNPLFAAKMVNI
                     MAARLANTNDLLFQKINELSAKKMEFQ"
     gene            31190..31720
                     /locus_tag="PLF66_08055"
     CDS             31190..31720
                     /locus_tag="PLF66_08055"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HMZ64030.1"
                     /translation="MNFISFFLSGGFLMYPLAAIAIINSTLILHLLTTFFIYLRDLRQ
                     IDKKNPKSSLIKSLVDISQKPGKRKFWIQFSDKLILYERRVFWLSNLSGVATLSGLLG
                     TVIGIYISFQNMKSSGTASAEVFADGISVALITTIAGLLIAIPSMISYYLLKHFLEVI
                     EEESYAFLFHSKANKD"
     gene            complement(31723..32160)
                     /locus_tag="PLF66_08060"
     CDS             complement(31723..32160)
                     /locus_tag="PLF66_08060"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HMZ64031.1"
                     /translation="MKNILVTVTFEEKETQILLEKARSIAEKFGSKLWLVHIAAPNPD
                     FIGYDVGPQYIRDHRAGELKKEHNLLKKYADEFKEKNIEAEGLLVQGATVDMILKESA
                     KLKIDLVIIGHHKHNLLHKIFIQDIDETIAHESNVPVLMVPFS"
     gene            complement(32187..32606)
                     /locus_tag="PLF66_08065"
     CDS             complement(32187..32606)
                     /locus_tag="PLF66_08065"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HMZ64032.1"
                     /translation="MKSAFNGLKVKDVMTKNVFTVNTHHTWQEVARKLSSKNIHHIVV
                     VDSNHKPVSVMSVSDFLRFAINSDKSILEKTLEEVKPHHRLISLKAEDHAYDAVNEMN
                     NHLVESVVVLDENGRLEGIVTSKDLMNVIFFDEKFQE"
     gene            33469..33828
                     /locus_tag="PLF66_08070"
     CDS             33469..33828
                     /locus_tag="PLF66_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008589019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLU-1-like domain protein"
                     /protein_id="HMZ64033.1"
                     /translation="MDFPDLESYFQVLTDTTDNIAIINTHYEADHDRDFKQLEDIFSD
                     IISRPWEHSPDDYYVLFTSYFTFHVKIIEEIIKEAREILNPEKRDYLKKLVNYKKEAD
                     EWFQALKKKRKSVMVAA"
     gene            33867..35297
                     /locus_tag="PLF66_08075"
     CDS             33867..35297
                     /locus_tag="PLF66_08075"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HMZ64034.1"
                     /translation="MVFNSIDFLLFFPVVYLLYLRINHKKQNILLLVASYFFYGYWDY
                     RFLSLLFLSTIIDYYASISIEANWDNPQKKKYFLYLSIFSNLTILGFFKYFKFFVKSA
                     VAGFSSIGIQVNEPVLNIILPLGISFYTFQTMSYTIDVYRGDLKPTRNFLDFALFVTF
                     FPQLVAGPIERATNLLPQILNPRTVTFEKVSQGSFLILFGLYKKVYIADNLATIVDPI
                     FLQNPESIAARTGAEAIIGCFAFLLQIYCDFAGYSDIARGIAKFMGVELIRNFNHPFF
                     ARDFNDFWRRWHTSFMNWLRDYVYVPLSKTNNSEFRNHVNMMIVFLLSGFWHGANWTF
                     IMWGFSLGVIASVYRIARRLNPNKEVGERKNPGALTWIFHAILADALVIFTCVFFRAE
                     ELSTGFIFWKKMFVEFGTIDPKILGKFFKLIIPLFLIELYQFKKDNEFAFFELKTVYR
                     VLFYLFLFYSIIIMGNNKNAFIYFVF"
     gene            35272..36351
                     /locus_tag="PLF66_08080"
     CDS             35272..36351
                     /locus_tag="PLF66_08080"
                     /inference="COORDINATES: protein motif:HMM:NF019243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1574 family protein"
                     /protein_id="HMZ64035.1"
                     /translation="MHLFISYSKKVPAFITTVILILGFEVSLYFVPNLHYLEGGGSFF
                     TYYKKNIAENEKQTFDILLFGDSRSLSINGIKEKEADYTMYNFSLPAAGPRYFKFFLK
                     KYLNHHPNPKLIIWAADPEQFNHTKSKTFNTDKNLWQQYKHRLLNLFTFLESFEQYSG
                     NELLFISREYLPLVMYSYKYRQGLDDLLSGLKLETITTRETYHTKQNRMIEAITSSHN
                     GQINLGDYFMADQKEVEKSYLKYIEALNAQKDFDLSPLADFISYAQNKNIPVLVLNIP
                     RAEGLNDTLYFKLITPEIKKIVSAYANARYLEFPQMDYPVSLFSESIHYNGAGEKQLN
                     SDFKKSIYPEIVSIIKKNEEASHEK"
     gene            36341..37399
                     /locus_tag="PLF66_08085"
     CDS             36341..37399
                     /locus_tag="PLF66_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015679227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1574 domain-containing protein"
                     /protein_id="HMZ64036.1"
                     /translation="MKNKKFLLYPVFLFLFLFAFDKIFLIDKVRSYVKSDFTYIYYET
                     REDLFESLKTRKSDKKLMIILGSSRLLYFDSKDLEQFYPNWDIYNFSSAVTTPAYYYY
                     YLEKILDAGIKPDFVLLESDPNQFNSNTPVFKNSNLTYSFNLPFILRHANLFGKEYVS
                     FYLGKFLFASSKNKPHLDTAYKRLTDPRYEVIAKEGHKIKEMLLESKGHSKSLVENFV
                     ERDFGILQATSQRTIDWLFSSYKESEMQYSFYEKILSKLSENKIKTVIVWPQSSLPMQ
                     DLMRKSETVGKKVSLWFTKVNSISGKYGYTVRDMDATEDYYCNTFVDGGHIAKDCYHP
                     FMRYVMSEYFKIAKEERQ"
     gene            37564..37971
                     /locus_tag="PLF66_08090"
     CDS             37564..37971
                     /locus_tag="PLF66_08090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64037.1"
                     /translation="MEKLYEKEGVVAIYWDPAARALCPEWKSFSVMDKEVKEGLEKLL
                     TFIIEKKCDRQIINTGKTLGAFSENIQDWLGNDWIPRAVKAGMKYVATVTPKNAMAQL
                     SNDYWQEVSTDIGFITINVTSHEDGVQWLKKFP"
     gene            38420..39937
                     /locus_tag="PLF66_08095"
     CDS             38420..39937
                     /locus_tag="PLF66_08095"
                     /inference="COORDINATES: protein motif:HMM:NF033566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LIC20035 family adhesin"
                     /protein_id="HMZ64038.1"
                     /translation="MYFLKTFLSLLPFLFLSCASLMQKDAKTEFSLETPNIRIEKFPG
                     TFNLKGKGPVKTNCAANNCSAEQISKLDAGSIKGFVKDGLWEEYTELTDKEDEKKKYS
                     VLWMKGSYKDGKKEGMWEEYEEKYDPTTKTKKLVKKQIGAFKENKKDGVWTLLYETGE
                     KLKETPFVDGKKHGTEKKYNQKGAQIEETNYVEGEREGVYWKKNSSELTECEGSYTKE
                     QKTGKWTEYNTDDKKPGKLKAVNLFVKDKKDGISTSYHADGSTKLAEGVYKEDFKTGP
                     WKQYYDNGKIESEGVKKPMPSDDEKDLKDQESSCPFPNKSGKSINFGEWKKYYKSGDL
                     FSIGSYDEKGLPTKEWKFYYKGNKLRCKGVMANALMMQSGELYDKTGNLEGKGNMMLS
                     MFNINEKTDEMEDKMIPALPFTFFKDGKKYMELLPATSGENNESGKTTEVRKQTTAIE
                     YDASGKKVGEGPYMFIPTQPYGGKKNGCWAENGKKVPYLMGKVQTGKLAEMSDCK"
     gene            40150..40311
                     /gene="rpmG"
                     /locus_tag="PLF66_08100"
     CDS             40150..40311
                     /gene="rpmG"
                     /locus_tag="PLF66_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004459639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HMZ64039.1"
                     /translation="MREIIKLACTGCETPGKSAYFQTKNKKLKSDKLVTKKFCKFCRK
                     HTDHKESKA"
     gene            40314..40769
                     /locus_tag="PLF66_08105"
     CDS             40314..40769
                     /locus_tag="PLF66_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002980616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HMZ64040.1"
                     /translation="MAAKSSQSKSSSSSKGFEELKEALLEKKNELLVKLNTWEDKSSP
                     SGLKEVGDIADIASEINEEALSSVLTENEIDLLNQIEIALEKIEKGTYGICEGTKKKI
                     PYARLKAIPWTPYTVEYAEIAAKQKAKISTRTSDTASYPAQPMDTELLD"
     gene            complement(40766..41506)
                     /locus_tag="PLF66_08110"
     CDS             complement(40766..41506)
                     /locus_tag="PLF66_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016751544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HMZ64041.1"
                     /translation="MKCIPAGNFIRGSNVYEKDEMPEQVIYVSEFYIDTYEVTNEDFN
                     KCLEAGKCKECLKNKTCNYIGPRYGRPYMGAKQPIAGVSWYTAKEYCEFLGKRLPTEA
                     EWEKAARGTNGDIYPWGNEPADCTRAVIEENGKKGCGKDKDLPTSDVGTRASGKYGLF
                     DMAGNSWEWVNDYYTKSFALCGADCSKKDPKGPCDGKEPCPGYNTRVLKGGSWWWDKS
                     YARGSKRRHHDPKNYPEYHHFGFRCAKD"
     gene            41810..43240
                     /locus_tag="PLF66_08115"
     CDS             41810..43240
                     /locus_tag="PLF66_08115"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HMZ64042.1"
                     /translation="MKTHTFNRTIGLTNAIILMMGNMIGIGIFVYPALISSLLPHSLW
                     FLFFWFLGGLIAVCGALSSAELASVYPELGGDYAYLRNSFGKRWAFLYGFFTFFITFP
                     GSIALGLSLAVHYQGSIIFGDWVNSVYYTIPAIGFELHFYQLIAIAILFILTIVNHFG
                     IEASIHFQKVVTFLPVIFLVGVGLLTIFSILYYLVLGNTSILRITDNLNMPLEFPNLL
                     PSGTALVAIYWTYAGWNSPLAMGEEIQNPEKVIPRTMILGPIVVMLIYLLLAFIFICL
                     LPYANIQSGTDPFFAVGQYFLKNLLKLHSETAIEWIPKIMTYIIFFIILGNTNSTIIT
                     GSRIQVAMSRDKLFFEKLGHLHEKTNTPVLGIWLQSIWALAMILLVNKESNLLNFSFI
                     CIIALSILTIYSVFLVKKKHQKPHLIPWISFPIAPIVYILATGLILILILGNYFREGN
                     FSIPVFALLTALIGLSLYEIWRKFKF"
     gene            43212..44780
                     /locus_tag="PLF66_08120"
     CDS             43212..44780
                     /locus_tag="PLF66_08120"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HMZ64043.1"
                     /translation="MKSGENLNFNSMCSLVTRCNPLYKLLILLAIFISFSCSIFSQEI
                     RYPNTPIYERKDLVPYKIYNKHQKKFRKLVKNFFHSMPPITHKGKDFTIYLLDKQEMN
                     ALEKSNLPYESFTKSAPFKYYDTSYDSRLKRKFHNLNDLNSGYKDQRLNKIYLQAIAK
                     EFPDKVKYFEIGKTRLGRAIPAVQITSPKKAEYKVPILFTGAHHANELISTEHCYDII
                     YHLLDDYDKYEPFLETISIWVVPIVNPDGSNFFWNYSIDMGRKNGYLPDDMRESNRTR
                     GVDLNRNYPFKWNTGHRFASSGDNTHHFYRGPSPGSEPETQAMMRLADLERFVFSMSF
                     HSFAAAILFPYTIENTFNPEPDYAKTLGQRLLRSAKSVRTGKEFALRKNLYPVDGTDQ
                     DFYYHEYGTIAYIAESSHQNVDYEIVQHILKGFKGVWETLLYEFFHGHKIVLRILDKE
                     GIPIEAKVEFPEIKYFEDEKFSSHPQTGIYIKMLPEEKEYKIHISKEGYEKQELVLKA
                     GKSMTPILVKMNPQ"
     gene            44777..46720
                     /locus_tag="PLF66_08125"
     CDS             44777..46720
                     /locus_tag="PLF66_08125"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HMZ64044.1"
                     /translation="MKKISYITALIIASYYLLLIFRFCHPYPKSEYYNVLGIQFLVVF
                     IAYLTKERYGRIMTVIAGFSLVRIGPILQISSLITITGVFFGASCGILFRELVLYFSL
                     KKDRELEKHSSSFLNPFLILIFFLYLLTLERFMIYFGSPFLNGFGILETLYLKILSSK
                     LAYSLSMETITNLFFPILYLFLEFTRNEKNKVIQDLKDGVKIVIALQFLIMTLQIVWN
                     RNFLADNTNLSLEANRVMGLFRDSGSSTWILPIICFLFYKDFLEKKKYIFFTLILVFQ
                     VYIASFQGRGFWLLFFLGFILQFLQYFKNVSIDSKKNFFYVFSISLLTIGFIALIPKD
                     KNGTLYRLLSIPNHFLEMVLSGKNPFLEFDPHRYYFNLAAWNLFQSHPLFGTGIGSYI
                     VNLKDSSLGLYIPENKIDNPSFYLGILSELGIFGLILIILPFWERILNKENISILILL
                     SVGLSFGYHIVQPDSAFALLLILIIGCKENSTELNHNFKIISMTILLSLYILYLSFQI
                     LSQDKMPEFRKFATGQFQLLAYELNKETENKKYHVFKGKTIWSIAKGIGGIEIDPFLD
                     NSTSKKTLKQRWSILDAKRNSLASFELTLEKEKEVLKLIRIPDNAYYLQVEELDEKGK
                     VQLYGDIPFCIPIIHFSEMNEFL"
     gene            46783..47151
                     /locus_tag="PLF66_08130"
     CDS             46783..47151
                     /locus_tag="PLF66_08130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64045.1"
                     /translation="MKNILLKNWKSNLIVYTKIYAILISTLFITCEIDFERNWCQEKL
                     ENREYAADPVFTLYTFCSIREKKNTESQEEYDAYVRNRTISCKESANLQILNYYLENQ
                     KKEECKKINRLLPTIHELTM"
     gene            47330..48352
                     /locus_tag="PLF66_08135"
     CDS             47330..48352
                     /locus_tag="PLF66_08135"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004779773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HMZ64046.1"
                     /translation="MFNLLEKDILPDFLIRLGIRNLLKARLEEEKKENLEEQQKHLNQ
                     YIEDLKKSPIAINTADANEQHYEVPTEFYKFVLGKRMKYSGGYWPLGAYTLDESEESM
                     LKLSCERAQLKDGLSLLDLGCGWGSVSLYVAEHFPKCKITAVSNSKTQKEYIDSVAKE
                     KGFKNLTVITRDMNEFTIDEKFDRIISVEMLEHMKNYQKLFAKLSSFLKPDGLFFIHI
                     FTHKQYAYHFEVRDETDWMAKYFFTGGMMPSHNLFLYFQENLNIVNHWVVNGTHYGKT
                     SEAWLANMDKHKSSILPILEKTYGKENVTKWWSYWRIFFLACAELFSYRKGEEWIVSH
                     YLFRKK"
     gene            48349..48885
                     /locus_tag="PLF66_08140"
     CDS             48349..48885
                     /locus_tag="PLF66_08140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64047.1"
                     /translation="MKYLIWSILLFTLQCSINSADIRKGNHLEKAQETSVEEFFERRM
                     KNRTKKVIGANWEIFFESEDFVYYGYPLVSFFREGTFVDEFFKVNRTKLQNDFPAFHE
                     FDGIKLRNLCYDSLAEYSRSTNDSLFQSFNCKAFLEQESIEIFADLTFKKENQYKIEK
                     YKIIFDKKKLKRESILKL"
     gene            48947..49837
                     /locus_tag="PLF66_08145"
     CDS             48947..49837
                     /locus_tag="PLF66_08145"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HMZ64048.1"
                     /translation="MKKMLVAFVVSLIFTTTANSEVIDNAGMFSTSTLTSANQKLDEV
                     KRKSGKELVIETIEEVGQADANALALEKAKARKVNGVYVLISKKDRKLKVEIGNKTKL
                     VFGESERNILKAKFAEEFKRQNFDQGLLSSVDYFSSVMDSAVASQTNNYSYPHRTDAP
                     IASRSSSGGFSWLSIIIFAVFAFIIFKLISTFLSNRNSGNINQPYGGGYGYGNSGGPG
                     FMGTFLTGLLGAAAGSWLYDKLTGHDSGLYGNDNSSYDNYRSNDSGWSQSDSDYSSDS
                     SDSGSDWGGSDGGDFGGGDW"
BASE COUNT        16519 a         8470 c         8371 g        16866 t
ORIGIN      
        1 tgaataagag gcggtatcta catcaataga aaatgtagaa agtggatttt cttttacatc
       61 taaaaaagga ttgtctgtaa ttttatcata ctgttcagta ttatactctt cgctatcaga
      121 aactccggat gcagtattca tctctggctt ggtttttggg ctagaaggct tttgatcttt
      181 tgtgtatatt ttagtgctct gttgcttatt tttataagat gcggtaggct tattcatttc
      241 tcttgcgggt tctgattgag caatggagta agtcttatct tcactcgcag aacaataaat
      301 tagcgagagg gaggaaagga ttgctaggta ttttattatt ttcatgattt tttatattcc
      361 tctaaaattg tatgagtagc agtgcgaatt gagagtctat tatccctaac gtcttcttca
      421 attttcttta tttttggatt tttttctttt aaggtttgta attcataata gatttgattc
      481 tgtaaataag accagagcca tttatcatat tgagatttgc ggttaatact catttgctca
      541 ccagaggcat ctatgaaatt ttgaatggtc tcccaaacag taggaattcc agttttaaga
      601 agagaactta ctgttaggac tggtgacttc caaaaactat tttttggctt aaataaacga
      661 agtgcatttt cgaattcatg ttttgcattt tctgcattga ttgtattcga tccatctgct
      721 ttattgatta gaattatatc cgccatttcc ataattcctt tcttgattcc ctgcaattca
      781 tcacccgctc ctgcgagtaa aaggagtaga aaaatatctg tcatatcaaa aaccgccact
      841 tcggattgtc caacacctac agtctccact atgattactt catagccagc agcttcacaa
      901 agtaatatag tatcttttgt tttgcgagca acacctccta ggtttccact tgtaggagaa
      961 gggcggataa aggcgttttc actttgagtg agttccggca tacgcgtctt atcccctaaa
     1021 atacttccac ctgtaatggg agaagttgga tcaaccgcta aaaccgcaac tttttttcct
     1081 tgcgagataa catgcatccc aaaggcttcg ataaacgttg atttgccagc accagggaca
     1141 ccacttattc caatccgaat cgatttagtt gaatgtcctg cgacacctga taagacctca
     1201 tctgataatt ttgcatcttc aaaacgactg ctttctacta gggtaattga ttttgcgagt
     1261 gctcttcgat tcccttctaa aattcccttt attaattcgt ctgtatactt catttctctt
     1321 caattctctt tcagtaaagg catatgtcaa actaactttt ataataaaaa atgacttgac
     1381 caagattcaa tatagtcata ttagtagtgt tatatgacta tggaggctga ctatgaacaa
     1441 ttatttcaat gtagcgcagg cgaaatccag attttcggag gttttgcaca gagccgagat
     1501 aagtccgcaa tttatttcaa gccgagggaa agatgtgggc gtagtattaa gtaagaaatc
     1561 ttatgaaaaa ctgctccaga tagaaaacga caatcttccc aagacaaagc taaaaaactt
     1621 ccttgctctt tccaaatctc ttcgatccaa tttcccgcaa aaactacctt cttctgctcg
     1681 aaaatcaaga caagcgccac aattttaaaa tttatttacc ttgagtaatt atctactaga
     1741 tacaaacgtt atttcggaac tcgcaagaaa aagtcccaat ccttctgttt tagatttttt
     1801 tgaaacaata gacgatatta cgatgagtgt agtcactctt gaagagattg aattcgggat
     1861 tgaaagactt cctgcatccc agcgaaaaac cttactcaaa tggtggtctg cacttctttc
     1921 tttgccgcct atcattattc ctgtagaaga gaaaattgca aaattagcag gacaacttcg
     1981 agctagacaa gaaagtaaag gtagaagtaa atcacaagcg gatatgttga ttgctgctac
     2041 tgccatatct aatgggctaa ttcttgttac ccgcaataca aaagattttt atgaattagg
     2101 tgttagtgtt ttaaatccat tttaggagac aaatatgatt caacgtgcta ttgaaattgc
     2161 actcgatgct cacagagatc aaacagataa aaatgggcaa ccttatattt tgcacgttat
     2221 gcgagtggga ttacgtggtc gaaccgatga agaaaaaatt ttaggtatat tacatgatgt
     2281 agtagaggat tcctcttgga catttgaaat gcttgcaaaa gaaggattct cggaacggat
     2341 tctgtctgct ctgaaatgtc ttacaaaaaa atcagaagat gaaccatatg aggaatttat
     2401 tggaagagta aaggaaaacc ctcttgcaat ttctgttaaa ttaaatgacc tagaagataa
     2461 tatggacgtt aggcgaatgg aaatcgttca agaaaaagat tccaagcgcc taaacaaata
     2521 tttaaaggcg tataaagagt tagctgaatt aaaaaatcag tgaccttcga attcgcatat
     2581 ggcatataca tttacgccat atttttcttt tatgatttta ctaccgttaa ggtcgggtag
     2641 atcaactatc acacctgctt catatacttc accaccaagg gctcttacaa gttgaatcga
     2701 agctcctaat gttccgcctg ttgcgatcaa atcatccatg attagaactt tgtctcctgg
     2761 cacgattgaa tctttgtgaa tttctacaat gtctgtgcca tattcaagat tgtattcttg
     2821 tttgattgtt tctccgggga gtttcccttt ttttcggatt ggaacaagac ctacacctaa
     2881 ttgaaaggca agcgctgcac cagtaataaa gcctcttgct tctattgcag ctacttttgt
     2941 aattccttgt ttttcgtatc ttgctacgaa agtgcctatc gttagagcga gtccttcggg
     3001 atctaaaata agcgaagtga tatctctaaa catgattcca ggtttaggcc agtctgggat
     3061 ggttctaatt tttgatttaa cgatagacat tgtattttcc ttgtatattt ccagattgaa
     3121 aaagattttc atttcgtaaa tcacaaatta aaagaataga cgagttcatt tttattggct
     3181 tggaattgca tctagtttac acaatacaca aattcttaaa aataaattgt caatctactc
     3241 gcattgtgta gtgtgagtga ggtttctttt taaaaagaat tatcgtttat gtctgaagaa
     3301 aaactttggt attcaaaaaa aaatgctgcg aattgtagtc aatgcttagc tcccgatagt
     3361 attgagattt attattacca acctactagg caaccagtga ttacaggaga tgaatttgtg
     3421 atcacatatc aagtaataca aaagacgaaa aaagtttgtg taaattgtaa ttttacagaa
     3481 gaattttttg aagaatggaa agagtataag tatccaaaga aagaagatat tgatttttta
     3541 aaacggagag caatttactt tcaaactaat tttaaataca aagcagagga ggcttttgca
     3601 gattatttag aaagaaatcc gtccgatttt gaaatgctct ttgagtttgg aagatatctt
     3661 ttggcaaata gagagattat aaaggcttat tcaaatttca aaaaatcaat cgagaaaaat
     3721 cctaattttc tacctgcaat ggaagcgatt acagatttac tttttcaact tttgaatgct
     3781 caaaatgact attggtttga atgccttcct tattatggat tttacagtca tatacatcta
     3841 acgtatcaaa attttttaaa tgaactaaat aaatatcttc gacttttaat agaacatcat
     3901 aagaataatc ttcaattact acctgcatat tatctgaaaa cagcaccttt attagaagaa
     3961 aaatttttat tccctctcgc ccttgagtat tatgaatacg ttatgagcca atctcctttt
     4021 aaagtagaac aagcggatgc aagccttaga gctggtctct tatacgccca gcttcaagat
     4081 tatccaaaag caatttctct tttggataat agcaatttgc tcctccctaa cctaatgata
     4141 cattatcata aagcgaaagt atatcgaaaa actgaggatt caatgaagag taatgcagaa
     4201 gtttctattt ttctaaaatc cttggattcc cttattttaa aaaatgaaaa tgatcctaga
     4261 ttgtattact ttaagggttt gggtttagaa ttgatccaag actctaaaga gttaattgaa
     4321 tactatcagt cagttattga aaaaggaaaa tttgatattc ctattcgaac tatctttcaa
     4381 agaaaaataa ataatctatt agatcctcct cgaagagaaa ttatttcttc gcaaaatttt
     4441 aactgtccag tttgtaaatc gaatgagttt attttttata cctatagaga tcaagttgta
     4501 ggatctatca agccaaaaga atctttgaat acagtttatt attacttaca ggttcttaga
     4561 aaaatatgca atcattgtca atttgaattt aaagaataca aatcttggga agatatagat
     4621 tttgatacac aaatttaaga ttgaagttgc ttgactccga ttcattctcc cttgaatgat
     4681 acatctatga atgcaatgat tattacaggg gcgagttctg gaattggaag acatttggct
     4741 tttgagtatg caagtcgtgg atttgctctt ggtttaacag cgagaagatt tgagcttctg
     4801 caaaaattga gaaatgaaat ctatgagaag tttggttcta atataaaagt agaaattaga
     4861 actttagatg taacagatta taaaaaaata tattcagttt tgaaggattt agctcattcg
     4921 ttaggaaata taactcatgt tgtggctaat gcagggttag ccggttctag tcctattggg
     4981 agtgggaaat ttctccatga taagtttgta atcgagacaa atttaattgg agccatggca
     5041 acagtagatg cagcagcaga aatattcaaa tcacaaaaat cacctggtca tattatcgga
     5101 atatcgtcta tcgctggatt cagaggattt ccagggaatg cttcctactg tgcatcaaag
     5161 gcaggtttta cagtattttt agaatcagct agacgagact taaaagactt taatatactt
     5221 gtatctacca ttcatccagg ttttattgca actgatataa atgcgcatat tccttctaaa
     5281 ccatttgtag tctcagtaga aaaaggtgct tcagaattag caaagctgat cgatcaaaag
     5341 gttatagttt catcagttcc taagtttcct tggggactta ttggattttt gttgaagtgg
     5401 attcctaacg ttatttggca gaggttaaag ttttgagcaa taaaataaaa gtgttatttg
     5461 tatgtcttgg taatatttgt agatcccccg cagcggaagg agcctttaaa agcttggtcg
     5521 agaaaaaagg aatctctgaa ttatttgaaa tagattcagc tgggactgca gggtatcatg
     5581 ttggagaaag accacatgaa acaacaacca aggttgcaag agatagagga attattttaa
     5641 atcattattg tcgccaattt gaaccaatgg attttcaaag attcgactat attctggcaa
     5701 tggatcaatc caacttcaga tcaattcttt ccatggcgaa aacagaatct gacaaacagc
     5761 gggtaatgaa gtttagacgc ttcgattcaa cagtcaaggg agagccagat gtgcccgatc
     5821 cttattatgg tggaatagct ggctttgagc atgttcaaga tattgtagag cgagcatctg
     5881 aaggtttttt aaattggtta ttagaaaatc atagcttacc aacagaatta gaggaaaatg
     5941 aggattaaac ccatggttga agaaaaacat atagtaattg tcggtgctgg ctttggagga
     6001 ttacaagcag caaagaaact aggaaataag aaggggataa aggttacagt ggttgataaa
     6061 aacaatcatc atttatttca accattgctt tatcaagtag caagttctgt tctaagtcct
     6121 gctgacattg cgattcccac tcgatcgatt actactagtt atgcgaatat atccataatc
     6181 atgtctgagt taaaagaaat taataaagaa catagaaagt tacacctaga gcacacaacg
     6241 atccaatatg attatttaat tctcgccatg ggagcaagaa caagttattt cggaaatgat
     6301 cactggaaaa aatttgctcc aggtcttaaa aatctaaatg atgccttaaa agtaagaaag
     6361 agtattctag tttctttcga gagagccgaa ctcgaaacaa atcctgcaaa agtagcagaa
     6421 cttctgaatt acgttgtaat tggaggaggt cctactggtg tagaattgtc cggtgcaata
     6481 gcagaactat cgcatcaaat cattcgaaat gattttagat tcattgattc atccatgagt
     6541 aagatcactt tgattgaagg tggtccaaga cttcttccta gcttctctga aaaatcatct
     6601 gatgttgcat ataaagaatt ggttaagagg ggagtcgata ttcgtttaaa cacaagggtt
     6661 gtgaacatag atgaagaggg tgttcatact ggtaaagagg taatcaaatc tcaaaatatt
     6721 atctgggctg caggtgttgc tgcaaatcca tttacacaaa agttagatac tcctttggat
     6781 aaatcaggaa gagtaattgt aaatagtcac tgtgcgattc catcacatcc agagatcttc
     6841 tgtattggag atatggcaaa ttttagtgag gggctagaaa gacctctccc tggagtctcg
     6901 ccagttgcaa tgcaacaagg aagatatgtg gctgatttaa ttatggatga aatcaagggt
     6961 aaatctagga aaccttttca ttacttcgat aaaggaagta tggcgactat cggtagaatg
     7021 gatgctgttg cagaagttgg aaatattaaa ctttcaggaa ttattggttg gctgggatgg
     7081 ctatttgtcc atttatttta tcaggttgga tttaaaaata aagtttccat cttgattact
     7141 tggatctgga gttatatttc ttttaaagca ggagcaagac ttatacaaaa tgaagaagat
     7201 taatcatctt cttctatttc tatatctttt gtgcttagag gacgaagtag ggaactattg
     7261 ataagatcat aaaccaaatt gtcaatgata gaaccttttt gccataaaga ctcagactca
     7321 gttcccaaaa cgtcttctag tagattatct gcatagattg ccatacattc atttgcattt
     7381 tcgtctggtg tcattagaat ctcatcattc tctgaataga taatattgat attatacgtt
     7441 gtgtaatcgg aaagattttc ccctcgatag taggacatta ttttatggaa attatccttt
     7501 gtatcgatca aaatttttac ttcaaatgaa ttttgttcta cacaatgaaa attgagaatc
     7561 tggcctaaat attcagacat agaaatagtt tttcaaataa agagacattg tcaaatgctt
     7621 ttttaagtta aaatcgaaag gaataaccgg ctatgtaata cgggtcatat ttgctgatgt
     7681 ggctaacgct tgttatgccc gactgggata caagttgata atccaggaaa aatcctctag
     7741 ttctgttatc tgtaaatgca ttttcattat aaaaataata tgcatcaaaa atattaaata
     7801 aatagatgag tgcaatcccg attccaatat ttcttgcaga gcttacatgg ctgttttcaa
     7861 cggatgattt ctgagcgtag tagattaaaa aagtgttttc aattggtggc ggtggattcg
     7921 aaaaatttaa caagaaccca cccagtaaaa ttggatttga aagtgaattg aaattattta
     7981 ctgcatttgc atgtttttgc ttctcactcg aataagctgc tccaccagca ataaggagga
     8041 gaggatacaa tatgcctgcc atataacgtc tctccataaa ctgaccgcat cctgggagaa
     8101 taatagaaca tcctgtaaca gcaagcctac tcggaatcga tcttttgggt tcttcccctt
     8161 tgctcggttc caaatttaca ttttgctttt cttctggtgg atctgcctct attgtaatag
     8221 gtttgggttc ctcttctttt aaaagaggtg ctttgtcctt agcatcagaa ctctcatctt
     8281 gtcttttttt tttatcttcc tctaattgat tgcttggtaa gatcggctct tctgacttgg
     8341 atttatcctc tactgattga ttaggatctc ggtaaatgat tttataaatc ttctctttca
     8401 gaatttctct taactcaccg ttaccgttaa cttcaacctt gattgaattt atattttggt
     8461 tcctgatttt accttgtatg acttctccat ctttcagcag aatcgtctct gcataatttg
     8521 agctcaaaat taatataaaa agtaaagtaa agacaatttt tctcataact ctattccttt
     8581 tttacaccct atgtaagcta ctcttaaaaa cgttaatctt ttaaaaatga gattggtaca
     8641 tacactatat atttagaaaa gtcgagtaaa atttcatttc caataaaaaa agtcacccac
     8701 tacaagcaaa aaagaccatt aaagccttag aaatgaaaaa ggttggtttg atttggatgc
     8761 aaaatcaaaa cggtttgtaa atttttatac ttagattttt agaaatgaat cagttaggca
     8821 ataaccaaat actttgatta tttcaatgct tcctgaaatg cctgatgaat tctctccaat
     8881 tcagattctt tttcattgat ccaatctaaa agccaaactc tataaaattt ccaaccgagg
     8941 gaggctaatt gttgttctct gagacgatcc ctttctttta cggtggggct tgacgcatag
     9001 tattcgccat cgcactcaat cgccaaaaca aactcatctt tttttggatg tctgacaact
     9061 aaatcaattt tgtattgaga aaaaccaaat tttggaatca cagtgtatcc atttttttcg
     9121 agtgtttcca taatatcttt ttcaatagga ttcaattttt cctgaccata ggatgcttgt
     9181 ggttttcccc cataaccagc ataattcaaa taagatttta aaagcctaac tccttctgaa
     9241 ttagaacgat ccaagtcaat atccgaatct ctaaatgagg aaacgatagt cattctcttt
     9301 cgggcacgtg ttatggcgac attgagtctt ctttccccac cttgatgagt aagtggtccg
     9361 aaattatggg gcaaattacc gttagcttct tttacatagc cgatcgataa tataattgca
     9421 tctctttcat ctccctgaac ctgttcaata tttttaatga aaaaatgatc ctcgatttct
     9481 tcattgaaaa atggctctaa atccattgaa ctttttaaag catcctctag agcagactca
     9541 attcgttgtg catgtgctat ccccatggta ataacaccaa gcgtttcatt tgggcgttct
     9601 gttgcatgat tcagaataag atcgattacc cgcttaactt ccatttcttc gctttcttct
     9661 ggcgtttgac ctatttcgaa ggggattaaa tcatgaaata aaactctctg atttttggga
     9721 ctagggaagg tgattaattt atttgcatag atattttcat tcgaaaatgc aatcaactcc
     9781 tcagatcggc tgcgatagtg ccaaactaaa tgtctttcat ttaaaaaact agccgttcga
     9841 ttgagtaaac tttcaaatcc tttagtatga gatacttcct ctgattcgtc atcgtcgtca
     9901 tcactttctc taaaaaaatc tgttggaggt aattgcttag tatcccctgc gacaacaatt
     9961 tgtttacttc gcataatagc tggaattgaa tctgctggaa taatctgact tgcttcgtca
    10021 aaaattacta catcaaaata ctgtttcttc gcatcaatta actgactgat agaaagagga
    10081 ctagccatcc agcaaggtct taaacttgtt aaaacgttag gcgtttcggg tagtagtttt
    10141 ctaagagata gcctcgcaga atttttattt gtttcgcctc gaactagatt ttcttcatca
    10201 ccgaattgac tcatttgttg catgacccat cttgcatgtc tatagttaag cctatctttt
    10261 gttaactcga ttctttctct gtctaattct tgaaattctt ttactaattc tgtttgtgtc
    10321 tttccatgaa aagtttttaa tttcccatcc tgtttatagg cttcttctaa acaagaatag
    10381 agccaagcgt attcaaattg cctcgcccaa aattctgatt caattcctct ttgttttatt
    10441 tcaattaaga atacggaagc attgtgttta ttaaactccc tttctatttc tttcacactg
    10501 cccaaagaca aagcttgtga atcattgttc aattctttga atatggaaat aatttctcca
    10561 atacccaatt tttcaatttg attctgattg attctacttt tgattttttg aatttcttgt
    10621 tgtaactggg caaaattaga actaatcgtt tcccaatctc ttactggctc aggataaata
    10681 gtcgacctgt gttgcgattt ccattctttc aatagatttg ctatattttc aatatcagta
    10741 aaaaagactg aactcgtttc ggctatcgga tttctagaat taaaaatgga ttcgtatgct
    10801 ttccagaaat ctgaatttgc aacttttaga aagaattcgt tatctctcag taattttata
    10861 attttcatat ctggaaattc atactgaggg aataattttt tccatttatt cagattcacg
    10921 gaaatttttt taatttcacc cagcaattct tctcgtgata cttcctcaga gaatctaatt
    10981 tttttcaaag tctctacagt ggtttttaat tctgaatcaa ataaattgga aaaccatttt
    11041 ttagattcgg cattgcttaa tatttgaatg tatttatcaa aatcaatatt atagatctca
    11101 tcagagtatt tatcagagat gcgactaata tctgttagta gattgatttt ttcatttact
    11161 aagaggggag tagtatccgg agttcgatgg atctgtttta aataatcaaa gccttgattt
    11221 aagtcagtgt cattcgcaaa ggcttttttt atagttaaga agttctttgt atctaaatga
    11281 aagatgtcat gattatctga atagagtgta tacattaagt ttactctttc cattaactga
    11341 atcatgtttt ccatttcttt aaaattttta ttatcactta atccataggc tcctaatttt
    11401 gaaaactcct gttccaagaa taaaagttgc ttatagattg tattgactga attggaaagt
    11461 tctgttccat cctcaacagt tgaaatatta gtattttgcc aaatggaagc ccttggattt
    11521 acaaataagg aagctagact tggcttttgt aactcttcaa gatgtgctct caaactatct
    11581 actacatcag gtgttaattt ctctagatct ttccctgtaa atttggtttg gcttttttct
    11641 gcctcggaga attgaatcaa tgcggataat aactcgtaaa cgcttttatt tgcaggactt
    11701 cttttcttgt gaaattgctc aacatgataa ttgagttccc ttcgtttctt tacatattgt
    11761 ttgtgtgtct caccaacatc aatgcttcta aaacttttta ttagatctag actttctttg
    11821 aattgatcta agaccatttt tctagaaatc cctgtcccat gtaaatctaa caaaagatgt
    11881 cccatgtttt tttggtcgag acggcgctta acgacttcga gagcagctct cttctctgca
    11941 acaaataaaa ctttctttcc gtttgcgaca agagttgcga ttaaatttgc gattgtttga
    12001 ctctttcctg ttcctggtgg tccttggatt gtgcaatttt ctccttttaa aacagcttgg
    12061 attacactct gttgactaga gtcacaatca aagacaataa aatcatcttc tggttgaatt
    12121 tgatcaaatt tttttggatc gatttctaca gtagatgttt taatttttcg aattgcaaac
    12181 tcatctcctg ccatagcaag aacaagttca gtctgattgt taggattttc tttttgaaat
    12241 tttaaatcct ccaccattga cattttctga aatgagaaat tgcttataat aaattgctta
    12301 tcaacttgaa tagattgaat tctttttgca gatagagtcc tcagtctaga gactaccgtt
    12361 tcaaaatcag gaacagaatc ttcatttaga ctgccattct catcttgcaa aagttgatcg
    12421 gaagttattt ttaaattgtg tcttttttct aaataagcaa gtagaactgg atttataaaa
    12481 gcatctccac tgcgtctgag gaatgctttg ctcttgctct ctatgctaat aggtagtagt
    12541 ataagtgggc accgaggagg acttgcatca tcatcttctt tccaagtagc aaagccgtaa
    12601 gcaagaagta atgtatctat ccctcgctct tctaggttct gcttcgctcg cttatttatg
    12661 attttaattt ttcggtatac gtctgcgtta ttgtctttga gggagagaag atcgttaatg
    12721 gaaacctttg atccaccaaa aaactctata aggtatttct cgtctaattt cgaaatatct
    12781 aacgttccga ctttttggtc tttgtaataa agtaagttat tacgacgcga taagtcagtt
    12841 aaatcctgaa tccattgttc acaagcttta tcaagtaatg tttgagagta gttcataatc
    12901 aggactatta aaaatttttc agtctttcga ttccaaattt tttctgtatt ctttcatcaa
    12961 tttctttcag agttgtttta tccaatacaa tctctgttcc attttctgtc aagaggctca
    13021 catacctttc tttatcagat gagatagctt tatctaaatc tcgaatagaa tttagagaag
    13081 attcattgac cgttttaatg atatcaaaac tcagagagtg atagccatta ttggaatcat
    13141 ctggtaaaac ttgaaccagc atcacataac gaccctgttc tccttttttg tgatatctat
    13201 aatagtcatt caagtagagc aactttttat caattctaga tctccagcta gaaccaaatt
    13261 ctttcatcaa aaactctgat aattccacaa aaataaagcc tccccgaatt agatattcag
    13321 gttctttgcc ttcattgttc atatatggta tcttgattgc cttgtaagga aatggcttca
    13381 atcgcatcga aattgtttct tcttttttat ttctaagaat ttttacagga atattttttc
    13441 ccattttaaa accaaattcc tctccgcaat gagcgaggaa ggatatatat tgtttaccat
    13501 aaattgggtg tttgaaatat ccttgggaat caattttcat cccaccatac tcgaggataa
    13561 catcatttag ctgcaaagaa ttatcggctg tagaataggg gagaatctct gcgaccaaga
    13621 ctccttgcat cgatttatct aagccaaaat actccttcgt tgcttcatct tgtaagggtc
    13681 taaatctaaa tcctttaaac ggaaagattg ttccagtggg tttgtcgaat ttttgcttta
    13741 taaaagtatt aatcaaaaat ccagggattg cctttcctga gtttttagat gcggtaaatc
    13801 taaataaaac tccaatcgct ttatccttat ctaaaattaa ctccccatct ccatctaact
    13861 tttcatttga attgatgttt aaatagggta attctgtata acccattggg tagttttcca
    13921 tttcaattcc aatcagtctt ccttttgcat tttgtataga tccagagttg tccaattgta
    13981 aaatggaaac tgctttgaaa ggatctatct tagattcaaa ttgaattggg actaaatcat
    14041 taaagaaatc tttatcgcta acttctaaaa cacttaaatt tgattcataa tccatcttaa
    14101 ttacttttgc aggaaaagat gtgtaagaag aaaacttttt tacttcgata gaagtgtaat
    14161 aatctattaa agatgtaaat acgattattc gcttatttgc aattacgatt cctacggcac
    14221 cagtagtttg tggctttttt gtttgccaag ggaaaagaaa gttaggcgat tgagttgtga
    14281 ttttaacctg cacaatgcta tttttaaatt tatcctctat tgtctctaag gagaataaag
    14341 atgaataacc aaataagaaa agaaaaagga aaatagcttt tttcatttgt tgcctacctt
    14401 ataaattgcc tgcacttctc gatctgcttt ttcaatatcc tcttttttta agataagtgg
    14461 aatatctata tctctaaatt ttatttttag aaatgaataa tcctttttag ctacaacttc
    14521 tttaaagtgt ttgatgtttt gaatctttgt atcgttgact gactctacaa tcatgttcaa
    14581 aaaataatct gagttcgtat taatcggatg cgctaatttt ctatacaata caacatcaga
    14641 atctacttct ttgcataagt catcgtctat gaaataaaaa aagcgataga gaaattgact
    14701 tcccccttgt gtttcattat tcttattcca ttcttggaga aggtctctcg aaattggctg
    14761 aaaaacaagt ccgcctaaca caaagtagtc gaatggctca tcgtatttgt gtcgcatata
    14821 agtaaattca tccattcgct tggcggggaa gttaacagtc atcatctgcc catctctaaa
    14881 aacacgaaaa gatatcgttt cgcctgaatg tttattgtct actatttcaa caaagtcaat
    14941 gcgggcatct ttatctagaa ctatcgttcc atttcgacca attggaagtc catcaatttc
    15001 gagtagtaaa tcatttttct taagatatcc ttctgcgctt ccgcctttta aaactttcgt
    15061 tacaaagact ccatttaaat cttttggaat ttttttaaat tcgcgtagag tgttattaaa
    15121 agaatttaaa gtccgaactc ctaattctac atacccatca tactttccat ctttgatatc
    15181 atttaaaaaa tagcgtataa cggtcgtagg aattaaatat ccaatattct cacctctact
    15241 ggcaacttga aatgcaactc caacaacttt attatcctgt attgccggtc cgccagagtt
    15301 gcctggatta attgcagcat caacttggat tactaagtga ctatcgactt ccgagtgtgc
    15361 gtaagtagat tgctcctttc tggatacaat tccgcgacta acggatattt tatctcctcc
    15421 aattggatac ccgacaacag taatagatga attcaaatcc ggaatggctc ctaattcaaa
    15481 agaagtagaa tcttcataaa aggaatcatc ttttgctttt aataaagcca aatcgcaatc
    15541 atgagctaca aataaaattt ctacctcata ccaagttgtt tgattgtatc tttgcacttg
    15601 aatgtattta gcatttgaaa tcacatgagc attggtcatt attttgtttt tatcaatgat
    15661 aaaaccagtt ccaccgctag ttctcacacc tgttgtcagc caaggagaaa aagcgtcaaa
    15721 accttgcgag taggttctaa tttggacaac gccttttctg actttatcaa aatcagagat
    15781 ttgttctgca aaaatcgaat tcaaaggaag taagatcaaa agtagtttca ggatatattt
    15841 cataaaattt aagttttctg agtcttctaa aattaagtta ttgctagaaa aatagatagg
    15901 attagaatat gtgtcataat ttactagctg aaagttaatt aacttgtgca tagtttagag
    15961 taaagattat ttttaaaaat gaatctaatt gttgaaataa tattctcaag cttgactatt
    16021 ctaattgtat ccattctcta tttagtttct gtatttagag ttccaattac atctacaaag
    16081 atcacatttg gaatatttgg ttttcttctg agtctctgga atttatttga gattctgcaa
    16141 aaacttcagc tggttcaaat tgattcttat tatttacaag aaatcatttt ttcaaatcta
    16201 actatttatg cgctcgttat ctttgcattg cattttcctt tttatagaag aagggaagtg
    16261 atgattacag tctatacagc atttgtaggt ggagttggct atctcataac aattcaaact
    16321 atatcgatcc cctatgtgag ccaatatttc ccactcgaga gtagatttta tgagagtatt
    16381 aatttttttc taaccagagc tttttctata tttgccgctt tatctttttt ggtgattatt
    16441 atttacaaac tcactcacgc aagtaaaggt ctcagaaaat ttttctatcg tgccttgtct
    16501 tattcaatca tactgaccat ttttattctt actctaaatt atattcttac ccttgattac
    16561 aaagttctaa aagcaaatat aaatgtttat ctaattgatt tattattttt atccatagtg
    16621 atcctttctc tgattcaatt taaattcata gcattttatc ctggatttct ttcgattatt
    16681 atttacagag agttgcctcg acttgtgatt caaggaatcg ctccttccaa tttagatggg
    16741 gcacaatctc taaaagaaga gctatggaaa atctacgaag tagagaattg gaaagctttt
    16801 ttaaatgaat tttggtttgg gattattatc gacgagaccc ttgataatgc ggtggaacat
    16861 ggaggaagga gatcggacga tgaaattaca gtgcaagttt ttgaaacaag cagattccta
    16921 gatatttatg ttattgatag tggaaaaggt tttgatccag agctagttcc aaatcctgca
    16981 agtccggaga gaatcaatat tcctacaggt agaggaattc atattatgaa aaaattattt
    17041 catgtaacct ggaatttttt gggcaatgaa attcgagtta gagtttccaa aaatccagaa
    17101 gacaatcccg aagaagctta aactaagaag ctgatagctt gagtagttac aattttttac
    17161 aaaaaaacag ttttcacaaa gcctagagaa gttattttaa gtaaaaatcc attagaggaa
    17221 taggacgaat gaaaaaatta ctattagttg caatgttact tgcaacatct atcgctttct
    17281 caaattgcag caaaccagct gtaagtgctg ctgagtgtga accagttgta aatcaaatgt
    17341 ttgaaaattt agcaaaagaa ctcaagccag aagaagctga aaaagtaaaa gctatggaag
    17401 caacattaaa agcaggtgtt gtaaaagaat gcacatctgg aaaatacaac ctagattgtt
    17461 taaaatctgc tacaaacatc gcagctctcc aaacttgtaa aaaataattt ctgattgaaa
    17521 cgagcaaaca aataacgtca attcgatgta ataatttttc accacggatg aacacggatg
    17581 gttttaaaat tctttgtttt ttatcggtgt cctcggtggt tacacattcg acaggctcag
    17641 tgcaagtcgg taattttatt gcatcgaact agcgttgctt aatatgcttg agttattaaa
    17701 tcccattggc ggtgaaactc actgccgatg gaatccattt cggctatcga tccgacaaaa
    17761 cataaaatcc ttttttatct gtatagcaac catgctctct ataaggtccg ctttctttta
    17821 gaaaatatat tttttgagca taacgctctc ttttctgcat acacctgaga caaatatcaa
    17881 atcgaattgc ccactcgcat tcttctggtc taaaaatagg aaagactctc agaagtggtt
    17941 ttgattcttc attttcggga gagttctggg aaaaaagggg agagaggaca agcaataaaa
    18001 agccgcaaag ggtggtagtt tttataactt ggatgaattt aaacaaatgt atcctctttt
    18061 tcgaatcaat aaggactgga aagaagtcta tttttcaatt tttaaatata ttttaaatct
    18121 ttttaactcc ttgactctca agccaatgag agccaaggat gaagtatagc caaaacatca
    18181 aaatcgttta ggctgctttt ttatgttttg aaatcaagtc gatgatctta tccataattt
    18241 gcattagatc aaagtctttc ggagtaaaga tatccttgat tccaatcgcg cgtaacgcat
    18301 caaaatcact ttcaggaata attcctccaa aaatcactgg aatatgctca aatgccttat
    18361 aatgaatcaa ttcctctttg atctgttctg cgatctcctt atgtgaacca gatagaatgg
    18421 aaattccaat tacatctgca ttttcttcga ctgcgctttg cacgatttct tgcggtgtaa
    18481 gtctgattcc ggaatagatt acatcaaacc ctgcatgtct agcggctatc gcaatcattt
    18541 ctgctccatt cgaatgccca tccaaacctg gctttccaac tacaatcctt ggacggtggc
    18601 cagtagtttt cataaactca tctactttag ttcggattac atttacaaga tcactttcaa
    18661 ttttcaattt ttgtccctct acaccagtag ccggttggta ttgaccaaat acttttctga
    18721 gagtatctgc ccattctcct gtcgtcactc ctgcctttgc gcattcaata gatgctggca
    18781 tcatattgat gttgtttctt gccgcatgtt ctaatgcctc gagtgctttt ttggctcttt
    18841 ctggatcccg ttttgttttt gtttcactta gagaatttag tgtcaattga gcagaagctg
    18901 gatctacttt gaatataccc ccgtcggtat cagtcataag tggcgatttg atcccatccg
    18961 tccatttgtt ttttccaacg attgtaattt cacccgaatt gatttttgcc attctttcgg
    19021 attgagattt tactaactgg gatttcatgt atccattttc aattgcttta attgctccac
    19081 ccatagctaa aatcttttca atttctgcat aggcttcttc tttgagtgac ttcactttgc
    19141 tatcaattac ttttgatcca tcaaatatat ctggatattc taaaaggtca gtctcataag
    19201 cgagtatctg ctggagtctc agagaccatt gttgatccca tggacgagga agggagagag
    19261 cctcattcca agcaggaagt tgaagtgccc tacaacgggc atttttagaa agaagaacac
    19321 ccaatgtttc agtcaaaatt ctccatgcgt tgttttctgg ttgctcttct gtgagcccta
    19381 gagagttgac ttgaactccg tagcggaata ttctatactt tggatttttt acattgtaac
    19441 gagtgcgggt aatttcatcc cacatttcac taaaggctct cattttacaa agttcttcaa
    19501 taaatcgaat ccctgcgttt acgaagaaag aaattcgtcc aacgcatttt tcgaactctt
    19561 cttcattaaa acaattcctt tcttttactg catctaggat tgccattgcc gtaatcaaag
    19621 caaatgccaa ttcttggaca ggagtagcgc ctgcttcttg taaatgatag gagcaaatat
    19681 tggcaggatt ccattttgga gaagccctga gagaatattc atacatatct actatgattc
    19741 gaatggattg ctctggtgga aaaatatagg ttccacgcgc tagatattct ttgataatat
    19801 cgttttgtgt tgttccctct aattttgaat aaggaacacc tgtctcgtct gcaagcgcca
    19861 tataaagcga cagtagccac atagaggttc cgttgatcgt catggatgta ttcatttctc
    19921 caatcggaat ttgatcgaat aacaccttga agtcatctaa ggagtttatc ggaactccta
    19981 cttttccaat ttctggtttt gcaattggat ggtcggaact ataaccacat tgagtaggta
    20041 ggtcaaaggc aatggataaa ccagtctgtc ctttggctaa attctttctg taaagttcat
    20101 tcgaggcacg agcattggta tgaccggcat aggttctaaa aatccaaggt ttatctttag
    20161 tgatatttcc attttcatca tagaggtgat gtgctttttt attttccata agtttccttc
    20221 attcgctatg aatttttgat attattaaat aagccatatt tttatatagt cctagagaaa
    20281 ttatgttagt ttacagagaa aatgagcctc cagtcaatta catgggattt ttgctctccc
    20341 tcatctttct ttttatttcc ctatttatat tttattcttt taaaggtgag ccaaaaaatc
    20401 aagactcatt tcttagactt gcaaatttaa aatcaaaagt tgaaaccggc gtgattcgaa
    20461 cctataccga gccggttacc ccatttctaa tcttcatttg gaagaaagtc acaggcttaa
    20521 gctactcaaa tgcatatcaa actttcatct gttttacatt gagtttattt ctccatatga
    20581 ttatgcttgt ttatcgaaag aatgagtggc ggttaaatca ttatttttta gtatatttag
    20641 tagcttttct accttttaca atctatttac ctttagaata tctagaagag atggtctgtc
    20701 tcattgtaat tcttctttta caagtagatt tcaaaatgga aaatcctggt gatctattta
    20761 aattaatcac aatgacaata ctcgcattta tgactcatct tatcatgttt atggtgggat
    20821 atactgtatt tgcaattcta aatgcaatcg attttatgaa tcgtcaaaaa gcgaagccaa
    20881 ccgtttttta caaaaagaaa aatgttgcgg gccagttgct attggtctac ctgattacac
    20941 tcatagtttt tgtgattatc gcaagttggt ttaagttttt tggtttagat tcttttcatt
    21001 tcctttggac aaatttctgg gattcgtttt ttaaattttt cccaattttc gttgttctta
    21061 aaattttaga gtatttactc agaaaagaaa aagagctaaa tacgttaggc actacaggaa
    21121 tcgtaatttt gctaattgtt gtttctggat actttatgta taaagcaaaa aatgaaaaca
    21181 ttgattattt agaaacgcaa aaaaatgagc tcttatctct aaaggcaaga ggtaaaattt
    21241 tacaaggtca agctatttat ggctatccat taattagcga ttatatgtat ttctatgcag
    21301 gagagactgt tttaacagca gaagtttctc gtatgaaaaa agacgatttt ttaataacgc
    21361 gtgggatggt tgtaagcgat aaacaaatac ttgaaagaag tattcgatct aggaatccac
    21421 tttttttccc acttgataaa gatacactgc tgatcggaaa agagttaatc gattttataa
    21481 tgttagaaaa agccaataca gaaacaaaga ccaagctctt tgaaacactc gcacaaattc
    21541 ctcccatcac accttataca agaatgaata aagcatttag tagcctattt ggattatgag
    21601 ttttgaaaaa aactactggt ctgaagttta ttcgggaaga ttgatagatg gaacattcaa
    21661 tgcagcaaaa catgcagatt atataaaaag catattttct ctctctgaat tccgcattcg
    21721 gaagataatt gatattggtt ttggaaaagg caaactatta gaagaagtat ctaagaagtt
    21781 ggagccagaa tcattgatag cattagatcc atcaaaactg atggtttcct ctttactgaa
    21841 aaaacattgg atttcaaaat ataatattgc catagtaaat aaaaaactag aagaatttaa
    21901 catagattat ctacagaagt atccatttga tctagcgatt gcgaattctg tctttcaata
    21961 tatagaagat ttagagggtc aagtcaaacg tttggcgtct attgctcgtt tttcgtattt
    22021 tagcgttcct actgataaag actatgttag aatggaagag gagttcaact ttaaagataa
    22081 atatgcattt gctcgttcca aaaaaacgta tttagattgt atttcaaaat actttactag
    22141 aatttccttt aatcttttag aaagtaagat agtaaaagaa cattcctatt ttcaagctga
    22201 gctctataga gagtaaagcc cctgatagac gcaggggcta atcgatgtta tttacataac
    22261 ggttgcgaga taatgttcct caatatgctc catacttttg agttctagtt ctatctttga
    22321 aagtttgctt tcatagtttg caatttcttt tcgtaactca gataagactt ttggactagt
    22381 cacttttaca tttctagcat agacacttac tggcataaaa tgaccaaatg tatcacgacc
    22441 atctatgcgt ttaacaaaat aataatcttt agctaaaaca tgcttagtag agtagtaatc
    22501 aggtgaagct atatctgact tttttgcatt ctttagaatg atcacaccat tgtaaacaaa
    22561 gcccatgttg tcttttgctt ttgcactatc tggaactgca cttccaaaga cgataagtga
    22621 atccttagtg gtttctgcga cttttccaat aatattaaag ctaacaggtg ttattcgatc
    22681 taatttagac tgaaggcttg caattttatt ttccaaaaag gatttatccg aaattaaacg
    22741 tttttccttt gcataaaaat ttctccaatc ttttctagat tctttcgtat tttccagtct
    22801 ttgattttct atgtttttga tgcgagcgat ttttacaaaa tcaatcgatt gataaaatgg
    22861 aggtttcccg ccacagatat ctgcatatat ggaaagatat tcatttaact cattttgtga
    22921 tttaataaac aaagccccat ttaaaaaaga agcaggataa aatttttttt cccctagctt
    22981 gtttcgtatt cctatttttt ttatggaatg aatcggatct atgtaaatgg attgctttgg
    23041 aatcgcaatg gtagataaat ttccaaaact tgggcaaact aggtcagagc ttaagaagac
    23101 tcttttttct atgcccaacc attttatagt gaatacaaat gcgattccta taaataggat
    23161 ttttgcgatc aatttgatat ttggtttcat gctcttctcc ctatgtattt aaaagtatat
    23221 cagtgagtag gacaagttag aggctttctg gaaaaaattc ctaaaaatat ttatagtagt
    23281 aaatatttgt ttatgctaca taatttaaga taaatgccgc tcagaagggg tttgagaatt
    23341 tctattctca taagctctat cttttttagt agaaaatctt catttactct aaaatttatt
    23401 ttaatttctc tctaacctgt cctacttaat cactactatt tatatatagt aaaattttgt
    23461 atagtaaaaa ttaaatgcta tacaaaattt tatattttta cttaacttca aaattatgta
    23521 agattaagaa ataaataaac ggagtcagat aactactatg aaaagaagga accttaccca
    23581 acaaccaggt tttggaaatt ttaaaaataa atgttcagat ttagtgagac gttatgatac
    23641 aaggattgtg cgaatgattt atgaagttgt atatgaaaat gtatattgga aatccggcac
    23701 aaaagattcg attcaaaaag aaatacttgc agaagtttat tctaaattaa aagaacaaag
    23761 aatctctatc ttacatttac aaatgattgt ttcagaaatg tatgaatatt ttggaaaatt
    23821 tttagtattg ctagattttg gaaaacgtag acaaaattaa tgatgaaagt cggcatataa
    23881 ttttttaaac atttgttttt ccaatagagc aggagcaaag agtcttaaaa ttttcacaaa
    23941 gaagcccttt ggatccattg taactaatct tgcatttgga tcctcggctg catctatcat
    24001 tgcctttgcc acctgattgg gagtgagtag attaccctta tattgtttat catttaattt
    24061 ttcaccagca cccgttaatc cagatgttct cagatttgtc tttgtatagg gtggacagaa
    24121 caaaatagaa cgaactccaa attcagaaag ttcaatccgt attgactcaa acacagcatg
    24181 caaagcagat ttagaggagg aataagcagc ccttccagga actccataga gacccgagac
    24241 tgtcgatgta actactataa cccctttggc tttttttatc gactcaatta attttgacgt
    24301 taggtgaact gttccaaaaa aatttatatc aaaggtcttt ctgaaaacac tgatatctgt
    24361 ttcgtcgaat ctaccatgtg tggtaatccc tgcattatta aataaaacat caacagtatc
    24421 tgtttttgac agtatctgct tttgtgcctc tattatatca gaatctaaac taatatcaca
    24481 ttgaatagga atgatttctg cgttgtgaaa gaattcaatt ttttttggcg atctgtctaa
    24541 ggcaaatatc ctacacggaa tatcttttag ttccattaat aaggattctc cgataccact
    24601 ggaagttccc gtaatcacaa tgtttttgtt ttgccaaaat ttaatcttca tcctaacctc
    24661 tactcacaat agagaatgca gaaataattc tcaaaagcaa tgatttttta tagaagtttt
    24721 aaattgtatc aagaatacct tcatttactg attcttctaa aggcagcatg acaataaaac
    24781 gactcccttc ttttaactta ctttctgcac ttaagtctcc accaagaagg tttgcaaatt
    24841 gctttgcaat tgttaaccca agaccagttc cagaatattt tcgtgtattt tcagcaaagg
    24901 attgctcgaa tggattaaaa attcgagtta aatcttcttc tgggattccg atgccggtat
    24961 cctctacctc aatttgtaac catttctttg aatcaatatc tttcgtttgg acggaaagag
    25021 aaatttttcc ttccgtggta aatttacatg cattagaaag tagatttaga agtatatgtt
    25081 ttagcttttc catatcttga tagatatatt gaatctcttc tgcaacagat agatcgaatc
    25141 tattatggtt tgcaaccaga agaggtttga ttgtatcaat taactcttct agaagtgtct
    25201 ttagatcaaa taaatgatag tttacttcta ctttacttgc atccaatttc gctatgttta
    25261 aaatatcatt gataagttta agcaaactct ttcctgattt atgtatcttg cgactatcgt
    25321 cgataatctg agttgcattt aattgatttg cttcttcttc aattaattca ctgtatccta
    25381 agatagattg caaaggtgtc tttagctcat ggctcattgc agcaaaaaag tgacttttgg
    25441 cttgcaatgc atcttctagt atatttttcg catggattaa atctgtttca acttttttta
    25501 atcgagtaat atcttgcatc tgtataatat aatactcggg taaattgttt ttatctcgta
    25561 acaatgaaat gctgagtaat gcccagacta acgcacctga tttatgttcc agcttacttt
    25621 ccatttgaag actctctact ttaccagaaa gcaaatcatc aaattttaga atataatcga
    25681 tcacaacatc tttttctaga aggctgtaaa attgacgaga gatcaattca tcctttgtgt
    25741 agccaagaat atttccaaaa ggctcattga ctctgatcca actatcatta atccctatta
    25801 aagccattcc tgatgcggaa tagtgaaaag cactttcaat ctgtttttta gcttcgtcta
    25861 gttgcacttc agtttttaga ttttctttta attcaggaat taaactacaa agagagattc
    25921 cgattccgac aaaacaaaaa gagactgcac tattcaaaga catcgggatg agattatgag
    25981 aatagaagag ggggacacca tatacataac ccaaagaaaa tattgttcca ataaatgcta
    26041 caatatttcc agagaaaaga gaaacatatt ctgataattt atatctttga ttagcggata
    26101 acagtaatat tccaatggaa cctaaaaaga tgcaaagtgc accaaaaaaa gaagttctag
    26161 ctgcataatt ataccacttc ccataatttt taaaatggaa aatgagataa tctacaccta
    26221 aatcaatttc tgtcacaaac tcaaacattc taaaaaaaga aattaggatt aaaaatatag
    26281 aagctatagt tagaaatttt ttaacccaat gaaaagaact gtcataaaaa aacaaacata
    26341 tacccaaaag aacggatgca agagctccgt taggcggcat aggtttgaaa ctaggattta
    26401 gacttaataa taattttgaa tttaaaatcc agccaatgaa agcaacaaca ccaattccaa
    26461 aggcaagaaa agagcataaa tcctttataa gtatatactg attaattttt gaacttaact
    26521 tcattcaaga atcctattaa tgattttgag acaataggac acgaatccat tttgttttta
    26581 ctatatcttc gggtgctact ttctgaaaga aatccccttt taaaagtata tgattttctc
    26641 ttgtgctaaa tgaatcgatt aactcttgaa tcatcccatc aagcaattca atcgcggatg
    26701 atgtataatt aaagtcacct gtcacaaaaa tcgtaaaatc tttaatatta ttagaataag
    26761 tttttagctg cataatataa tttggaagag cagttctaaa ttgggaaggc gttgcaaaac
    26821 ctttaataat taatttaata gatgattcat caattacatc atgagaaatt gaaaatggat
    26881 agtccttttt taaaatcaaa aaggaaggat caattcccat tgcctcaatg accttattca
    26941 caagctttgc ttgttcaaaa ggttttacta aataatgcgt aatgcgtaat gcagctgctt
    27001 ttagaatgtc ttctttttct tgcaatgcag ttagaatgat gattggggta ttttgaattt
    27061 cctctcgatg tttttcaata aatgtaaatc catccataat aggcatcatt acatctgaaa
    27121 taatcaaatc atatttattt ttgagtttac ttactgctaa acgagcattt gcaacatggt
    27181 ctatatggat attatatttt tcgagggatc ttgttacaat ttcagctgaa tttgcatcat
    27241 cctctaaaag taaaatacga tatgttctat atttatcttt ctgctttggc tccattatta
    27301 tatccttttt ttgataattg tcttacgttc actcatctct tccattgcaa aataaattcc
    27361 ttctctccct agaccagatt ctttaatccc cccataaggc atatgatcta ctctaaatgt
    27421 aggaacttca ttgaatatga ttgttcctga ttcaatttcc tctgtcgctt gtcgcattat
    27481 cttatcactg tcagtaaata ctcctgtttg aagtccaaac ctagattcat ttactctttc
    27541 aattgcttcg gcaaaatcga aataactatc taacattaca ataggaccaa aaacttcaga
    27601 ttcatagact ttgatatgag taggaactga ctctaaaaca attggataga ttatattatc
    27661 aaccgcattt actgagttca aaaaaacagc accttcttct tttgcttcct ccacccaagc
    27721 aaaaattcga tctcttgctt ttttatttat caaaggtcca atcgaagtct ccttttgaaa
    27781 aggatcaccg gcaatcattt tttccgtctc tcttaaaatt ttccgtttaa attctttata
    27841 tatatcatgg tgaataaata tcctttgaac agagatgcaa atttgcccag cataagagaa
    27901 tgctcctaat acacagcgtc tgacggctaa atctatatca gcgtctttat gcactattac
    27961 agctgcattt cctccaagtt cgagagatat tttcttacct gcggctaatt tctgtaattc
    28021 ccaaccaact ttgtcgcttc ctgtaaaact tactacagca actcttttgt cttttaccat
    28081 cttcgaagca agatcatttg aacaatgaac taattgtaag gcagtggatg gaattttctc
    28141 aaattcattt gaaactagtt ttaccgactc tagaaatact tccgtcaata ggttagctgc
    28201 tccaggtgct tcgggagatg gctttaatat tacagtagcg cctatcgcta atgcaggagc
    28261 aactttatga gctacaagat tcaatggaaa attgaatgga gtaattgcta aaacgacacc
    28321 tttaggaaca aaataactaa atgccggatc aaatccaact ccgcttttat caccatcaag
    28381 agaaatcatt tccccattat aacgctttgc ttcttcacta gcaatttgaa aggaggtgat
    28441 tcctctattg acttctcctt ctgcaaattg gataggttta ccggcttcat tgacgattgt
    28501 ctctatgaat tctttttttc tagattcaat tcctgctgct atgtatttga gtaataatga
    28561 tctagcgtaa cgagaagatt ttttaaaaaa aggaaatgtt tcataggcag ataaaattgc
    28621 tttatctatt aatttctcat ttgcctgctc acagagtgct acagtggttt gagtgtatgg
    28681 agagctaatc actttagtgt ctcccgtttc ctcccaatct ccattgatcc ataatttata
    28741 gcgcatactc actcatggtt taaaattaaa atcagatagc aagaagaatt ccattaaaaa
    28801 ctcacaccac attgtgaggt gatttgtgtt ttatactttg gataaatagt aggattataa
    28861 tgaatggaat cgatcatcaa agaagaatct ggtgggtcat tttccccaac tccaaaaata
    28921 ggaagtggat tgatataaca aactcctaat tgacttactc cttggtttac tggattcttt
    28981 aaactatttt cactcggcag taaaatcgga tgaatcccaa tcactacaat gtttgcattc
    29041 catttccctt ttatctttgc aattacctta cgcattgttt caatactatt cgcactggaa
    29101 acttttctca aaacaccatt tccatcacca gttgcgatta aaacattctt ggggttacag
    29161 cgaatatttt cgatttggta aaagtaatca cacgctgtat ttcctgatat cgcataattc
    29221 aaagttttag tggcatcata gaagctagtt gcacgagcca agtccatcgt tgaatctcct
    29281 agaattatcg catcacattg agtagagtag gatgaaaatg ttgcagatgc ataagtcgcg
    29341 taaagagagt ttaaatattt gtattgatct ttacaatctg ttgattgagt tttttgatat
    29401 gtgtaaagag ctgcaaatag gattagatct ttcttttttg gatttctctc tgaatgattt
    29461 tggcaataaa taaaaatcaa acaagaaata cagaataccc atctgtttct aaatttcatc
    29521 tcaatcgaat tggatttact ctcatttctt ttctgaaaat ttatcaataa taccataagc
    29581 catcttccaa acaaggagag gcaaaataat attaatcgca aatgtaaaga tccatcctaa
    29641 ataaaaacca cccatataat aaggatccaa catatgcatt aaatgaattg tcattggatg
    29701 taataaaaaa agtgccaagc tattttcacc gataaataca aataccgaat agattttttt
    29761 taggaaagtc tgattcgtag attccagtcg tattccaatc agggtaaaaa caatgaaaca
    29821 agcaatcgga taaaaaagaa aatgattcga tatctctaga tttaaatcaa ctgtataata
    29881 agttacaaca gatagataaa ttaaaatagt aattacaaga ccctggaagt aatatctatt
    29941 agctgataaa ctatctaaga atagattttt atttgtaaga agtcttttgg cgctaaatcc
    30001 agaaacaaaa aagaatagat agtttgtaaa actaatcgcc tccattcctc caatataagg
    30061 tagagatttt ggataagagt gatttgacca cacattgatc aataaagaaa gaattaaaac
    30121 tagatagaat aaattcgcac tccagttttg tatgtttcga aaaaataatg agtagagtaa
    30181 gtaaaacgaa aatagcaaag gaacgtaata atagggttgg aacacttgac ctagcaataa
    30241 tttaggaatt atgtctgata ggaaagattc cgctggatat ttagtaaaaa aaccaataca
    30301 agcaatgatg agatagggaa taagtagatt ttttatcttg gaattccaaa atgcagagaa
    30361 acccttccat gataagaaaa aaccagatga aaagataaag gctggaacac aaaagcgtga
    30421 gaaattagcc attaatttag atagaattaa ctcgctatcc tttgggtgaa aaaaagtaaa
    30481 ataagagtca acatgtatga ctacaatcgc gcacattgcg acacctctca caaagtccac
    30541 aactctactt ctttctcctt tcttttcagg aggggagttg gaaaaagtaa ataaatttct
    30601 gaatgactgg aaagaaacta gtaggattaa taaaagaatg agtccaaaaa attcgatata
    30661 taaattagtt gtttgtatca tagtaatata agaagttgtt catattgggc tgaattgagc
    30721 ctattgtata aaacaaatat gttcaagaat ttttttttag ctcaaatgtt ttccgaatcg
    30781 aggaactaaa tggcatctat agttagttta aaagctggta aaactatatt caaagaaggg
    30841 gactttggaa agtcgatgta cattgttcta gaaggatctg tagaaatcta cgttgttctg
    30901 aataaacaag aaacacacct tgctagtatg aatcagggag atttttttgg agaaatggct
    30961 ctttttagag ataagcctcg cagtgcttgc gcaagagtaa tttcagatgt caggttagcc
    31021 gtgatagaaa gtcgtgccca attagaaaaa tttctttcgg ataacccctt atttgccgct
    31081 aagatggtaa atattatggc agctagactt gccaatacaa atgatttatt attccaaaaa
    31141 ataaacgaat tatctgcaaa gaagatggaa tttcagtgag tctctctttg tgaattttat
    31201 ttcttttttt ctctcaggcg gttttctgat gtatccatta gccgctattg caatcatcaa
    31261 ctcaactctt attctacact tacttacaac gttttttatt tatttaagag atttacgaca
    31321 aatagataaa aaaaacccaa aaagttcact cataaaatct cttgtagata tttctcaaaa
    31381 acctggaaaa cgaaaattct ggatacaatt tagtgataaa ttaatcttgt atgaaagaag
    31441 agtcttttgg ttaagcaatt tatccggtgt agcaacgtta tccggtctac taggcactgt
    31501 aattggaatt tacatctctt ttcaaaatat gaaatcaagt ggaactgcat ctgcagaagt
    31561 atttgctgat gggattagcg tggctttaat tacaacgatt gcaggattat taatagcgat
    31621 tccttctatg atttcctatt atctattaaa acatttttta gaagtcatag aagaagaatc
    31681 gtatgctttt ttatttcact ctaaggcaaa taaagattag cctcaggaaa atggaaccat
    31741 taaaacagga acattggatt catgcgcaat ggtttcatct atatcttgaa taaaaatttt
    31801 atgaagtaaa ttgtgtttgt gatgcccaat gattactaaa tcaatcttta atttggcaga
    31861 ttcctttaaa atcatatcaa cggtagcacc ttgaacgagt agtccttctg cttcgatatt
    31921 tttttctttg aactcatctg catatttttt gagaagattg tgctcttttt tcaattcacc
    31981 cgcccgatga tctcgaatgt attgtggacc aacatcatat ccaataaaat ccggatttgg
    32041 tgcggctata tgcacaagcc ataacttaga tccaaacttc tcagcaatgg atcttgcttt
    32101 ttctaaaagt atttgcgttt ctttttcttc aaaagtaaca gttacaagta tattcttcat
    32161 gtttctaatg cctttcaggt atctttttat tcctgaaatt tttcgtcaaa gaaaattaca
    32221 ttcatcaagt ctttactcgt aacaataccc tctaaacgac cattctcatc taaaaccact
    32281 acagattcta ccaaatggtt gttcatttca ttcactgcat cgtaagcatg atcctccgct
    32341 ttcaacgaaa tcaagcgatg atgtggcttt acttcttcta aagttttttc caaaatagat
    32401 ttatcactgt taatagcaaa acgtaaaaag tccgaaacgg acataacaga aacaggttta
    32461 tgatttgaat cgacgacaac aatgtggtga atgttttttg agcttaactt tcgagcaacc
    32521 tcttgccaag tatgatgagt attgaccgta aaaacattct tggtcataac atctttgact
    32581 tttaatccat taaatgcact tttcataact tactcctctt agcggtagac tattttatca
    32641 gggaatatgg atattgcaat gatttttgaa attaaaattg atctcaatga aaaaagaatt
    32701 gatgcgatag atggtattta tcacaaaaaa ctatgacgat ggaaaaatcc ttgatactac
    32761 tttggatttt aacataacta aatccaatat ttcccgaggg aaatgaataa atcaaaatca
    32821 ctttttcttt aaaaatgcaa cacttacgtt taacggtgaa cggcgcacgt aaatctcctg
    32881 cattagttga ggaaattcca ttgaacacta aaattcaaac agacgatatg ggatgtctcg
    32941 aattcaaata caacgaaact acctatccta aaagcaagaa taccacagca ctcgcattta
    33001 attaaatcaa gctatcctag gctaaatact acaaaccaaa gtctgaaaat ctaggttcct
    33061 agatttaaca agaaaggaaa gaggaacgag gacagaaaag agaggataaa tattattatc
    33121 cgcaagttat taaaaattgt atttcaaaaa ttaaaattgg atttgaacgt tattcactaa
    33181 aaaatgatcg ctttatcatc aatacgattt tttcagagaa tgatacaatg caaatgaaat
    33241 taacgatagt gtaaacacat tagactggaa ataaattata attactgcta tagatttttt
    33301 ctgattatgt ctttccattt aaaatttttt ttaataaata tacattttta agtagacggg
    33361 taacaggtat taaccccata attgtcttat aaataaaaat aaaattgatt gtagtacaag
    33421 aggcttctaa aaatatggta aaaaggcagt aaaaggaagg tacgaaagtt ggattttcca
    33481 gatttagaat cttattttca agttttgacg gacactactg ataacatagc gatcatcaac
    33541 actcactatg aggcagatca tgatcgagac tttaaacaat tagaagatat ctttagtgat
    33601 atcatttcac gcccatggga gcattctcca gatgactact atgtgttatt cactagctac
    33661 tttacatttc atgttaaaat aattgaagaa atcataaaag aagctagaga aattctcaat
    33721 cctgagaagc gcgactatct caaaaagctt gtaaattaca aaaaagaagc tgatgagtgg
    33781 tttcaggctc tcaagaaaaa aagaaaatcg gtcatggtag ctgcataaaa tttgattttt
    33841 aagacatacc atagagtatt gactctatgg tatttaattc aattgatttt ctcctctttt
    33901 ttccggtagt ttacttactc tacctgagga taaatcataa aaagcaaaat atcttactcc
    33961 ttgtagctag ttattttttt tatggctatt gggactatag atttttgtcg cttttgtttc
    34021 tttccactat tattgattac tatgcttcta taagcataga ggctaattgg gataatccgc
    34081 aaaagaaaaa atattttcta tatcttagca tcttttccaa tttaactatt ctaggctttt
    34141 ttaaatactt taagttcttt gtaaaaagtg cagtcgcggg tttttcatca attgggattc
    34201 aggtgaatga acctgtcttg aatatcattc tacctctagg aatttccttt tatacatttc
    34261 aaaccatgtc atatacgata gatgtatata gaggtgatct aaaacccact cggaactttt
    34321 tagattttgc cctttttgtt acctttttcc cacagctagt cgcaggtccg atagagcgtg
    34381 ctaccaatct acttccacaa atactcaatc ctcgcacagt tacttttgaa aaggtttcac
    34441 agggttcttt tttaatttta ttcggtcttt ataaaaaagt ctatattgca gataatcttg
    34501 caactattgt agacccgatt tttttacaaa acccagaatc cattgcagcc cgaacgggag
    34561 ctgaagcaat cattgggtgt tttgctttcc tattacaaat ctattgtgat tttgcaggct
    34621 attcagacat agcacgtggt atagccaaat ttatgggagt agagctgatt cgaaatttta
    34681 accatccttt ctttgctcgg gacttcaatg atttttggcg tcgttggcat acatccttta
    34741 tgaattggtt acgcgactat gtctatgttc ctctctccaa aacgaataat agtgaattta
    34801 gaaatcatgt taatatgatg atagtttttt tattatccgg tttttggcat ggagccaact
    34861 ggacatttat catgtggggt ttttctttag gagttatagc aagcgtctat cgtatagcgc
    34921 gtagactcaa tccgaataag gaagtagggg agagaaaaaa ccctggagca cttacatgga
    34981 tttttcatgc gatattggca gatgctcttg tgatttttac atgtgtattc ttccgagcag
    35041 aagaactctc tactggattt attttttgga agaaaatgtt tgtagagttt ggaactatcg
    35101 atccaaaaat tttaggaaaa tttttcaaac taatcattcc acttttccta atagaacttt
    35161 accagtttaa aaaagacaat gaatttgcct tctttgaatt aaaaacagtt tatagagttc
    35221 tgttttacct gtttcttttc tactcaatta taatcatggg gaacaataaa aatgcattta
    35281 tttatttcgt attctaaaaa agtcccggca tttatcacta cagttattct aattttagga
    35341 tttgaagtta gtttgtattt tgtcccgaat ctgcattatc tagagggagg gggatctttt
    35401 tttacatatt ataaaaagaa catcgctgaa aatgaaaaac aaacctttga tatactttta
    35461 tttggcgata gtcgctcttt atcaataaat ggaatcaaag aaaaagaggc agattataca
    35521 atgtataatt ttagccttcc agcagcaggt cctcgctatt ttaaattctt tttaaagaaa
    35581 tacctaaacc atcatccaaa tcctaaactt atcatctggg ctgcagatcc agagcaattc
    35641 aatcacacaa agtcgaaaac ctttaacaca gataaaaatc tatggcagca atacaaacat
    35701 agactcctga atctttttac atttttagaa agttttgaac aatatagtgg aaatgaactt
    35761 ttatttatct caagagaata tttacctctt gttatgtatt cttacaaata tagacaaggc
    35821 ttagatgatt tactcagtgg tttaaaacta gaaactataa ctactagaga gacttaccat
    35881 acaaaacaaa atagaatgat tgaagcaatt acctcaagtc acaatggtca aattaattta
    35941 ggtgattatt ttatggcaga tcagaaagaa gtagaaaaaa gctatctaaa atacattgaa
    36001 gcgttaaatg cacagaagga ttttgatcta agtcctttag ctgattttat ttcttatgct
    36061 cagaataaaa acattccagt tcttgttttg aatattccta gggcagaagg attaaacgat
    36121 accctgtatt ttaaattaat cacaccggaa attaaaaaaa ttgtttctgc ttatgcaaat
    36181 gcaaggtatt tagaatttcc gcaaatggac tatcctgttt ctcttttctc ggaatcaatt
    36241 cattacaatg gagctggaga aaaacagcta aattctgatt ttaaaaaatc catctatcct
    36301 gaaattgtat caatcataaa aaaaaatgaa gaggcgtctc atgaaaaata aaaaattttt
    36361 actctatcct gttttcttat ttttattttt attcgccttt gataagattt ttctaatcga
    36421 taaggtgcga tcttatgtaa aatcagattt cacctatatt tattatgaga caagagaaga
    36481 tctttttgaa tccctaaaaa ctcgcaagtc tgataaaaag ttaatgatta ttcttggatc
    36541 ctctcggctc ctttattttg attcaaaaga tttagagcaa ttttatccca attgggatat
    36601 ttataacttt tcttctgcag taacaacgcc tgcatattac tactactatc tcgaaaaaat
    36661 tttagatgct ggaattaagc ctgactttgt tcttttagaa tccgatccaa accaatttaa
    36721 ctcaaacaca ccggtattca aaaactcaaa tttgacttat agctttaatc taccatttat
    36781 tctaagacat gcaaatttat tcggaaaaga atacgttagt ttttatttag gtaaattttt
    36841 atttgcttca agcaaaaaca aaccccattt agatactgcc tataaaagac tcaccgaccc
    36901 cagatatgaa gtcattgcga aagaaggtca caaaataaag gaaatgctct tagaaagcaa
    36961 aggacattct aaatctctag tggaaaactt tgttgagaga gattttggaa ttttgcaagc
    37021 aaccagtcaa agaacaattg attggctttt ttcttcctat aaagaatcgg aaatgcaata
    37081 ttcattttat gaaaagattc tttcaaaact atcagagaat aaaattaaga cagtgattgt
    37141 atggcctcag agttctctac caatgcaaga tctaatgcgc aaatcagaaa cggttggaaa
    37201 aaaggtaagt ctttggttta caaaagtaaa ttcgatcagc ggcaaatatg gatataccgt
    37261 tcgagatatg gacgcaacag aagattatta ttgtaatact tttgtggatg gtggacatat
    37321 tgcaaaagac tgctatcatc cttttatgcg atatgttatg agtgaatatt tcaagattgc
    37381 caaagaagaa agacaatgac aaaaaatgac tctttcaaat atatctattt agcctaatta
    37441 aaaattgaga aaaactcaaa attaattttg gtatgtaatt tataaaaaat ttattgaact
    37501 tacttatatt tttaaaaata tagattccat ttttaaaaat gaattttgac aatgaggatt
    37561 taaatggaaa aattatatga gaaagagggt gtagtagcaa tctattggga tccagctgct
    37621 agggctttat gccctgaatg gaaaagtttt tctgtaatgg ataaagaagt aaaagaagga
    37681 ttagaaaaat tgctcacatt tatcattgaa aagaaatgtg atcggcaaat tataaatact
    37741 ggaaaaactt taggagcatt tagcgaaaac attcaagact ggttgggaaa tgattggatt
    37801 cctcgcgctg taaaagcagg aatgaaatat gtggcaactg ttactcccaa aaatgcaatg
    37861 gctcagctat caaacgatta ttggcaagaa gttagcactg acatcggatt tataacaatc
    37921 aatgtaacct ctcatgaaga tggagttcag tggttaaaga aattccctta acccctaatt
    37981 tacactgaat gaataatgat catagacata aagtccgtct atgaatcgaa tcaagccaag
    38041 catcctgtta cctatatcgc atttagttct aaatacattc atactatcta taactgaacg
    38101 ttctcaaagg atgtgcatca aagaaatatc gtatcttttc tcaataaatg gagctactct
    38161 tctgctccat attttttata ctaatgaaag cgcaatagga atcattaaaa agttattgaa
    38221 tcattttata taccgaatga caaaaataat tttccataag ggctttattt gagtagtatt
    38281 tggtataccg cacatacaga cgtgtgggaa cactaagaag tgtttagacc aaacccacct
    38341 ttttattttt gaaatgggat ttttcttttg acgtttggta taaatagaaa atgataggag
    38401 taacaaaaat gaggtgttca tgtatttttt aaaaactttc ttatctcttc ttccattttt
    38461 atttttatcc tgtgcatcgc taatgcaaaa agatgcaaag acggaatttt cattagaaac
    38521 tcctaatata cgaatagaaa aattcccagg gactttcaat ttaaaaggaa agggacctgt
    38581 aaaaacaaat tgtgcggcta ataattgttc agcggagcaa atttcaaagt tagatgctgg
    38641 ctctatcaaa ggatttgtga aagacgggct ctgggaggaa tataccgaat taactgataa
    38701 agaggatgaa aagaagaaat acagcgtact ttggatgaaa ggtagttaca aagacggcaa
    38761 aaaagaggga atgtgggaag agtatgagga aaaatatgat ccaactacca aaactaagaa
    38821 attagtaaaa aaacaaattg gtgcctttaa agaaaataag aaagatggag tttggacact
    38881 tctttatgaa acaggggaga aattaaaaga aactcctttt gtagatggaa agaaacatgg
    38941 cacagaaaaa aaatacaacc aaaaaggtgc gcaaatagaa gaaaccaatt atgtagaagg
    39001 ggaaagagaa ggagtttatt ggaaaaagaa ttcttctgaa cttacagagt gcgagggaag
    39061 ttatacgaaa gagcaaaaga caggtaaatg gactgaatac aatactgacg ataaaaaacc
    39121 tggaaaatta aaagctgtga acctgtttgt aaaagataaa aaagatggaa tatccacttc
    39181 ctaccatgct gatgggtcaa caaagttagc agagggtgtt tacaaagaag atttcaaaac
    39241 aggtccctgg aagcaatact atgacaatgg caagatcgaa tcagaaggtg taaaaaaacc
    39301 tatgccgtct gacgatgaaa aagatttgaa agaccaagaa tcgagctgtc catttccaaa
    39361 taaatcaggt aaatcaataa actttggtga atggaagaag tattacaaat ccggagattt
    39421 attttcaata ggatcttatg atgaaaaagg tcttcctaca aaagaatgga aattctatta
    39481 taaaggaaat aaactgcgtt gcaaaggagt aatggctaat gcacttatga tgcaaagcgg
    39541 cgaactttat gataaaacag gaaacttaga aggaaaagga aatatgatgc tttctatgtt
    39601 taatataaat gaaaagacag atgaaatgga agataaaatg attcctgctt taccatttac
    39661 cttttttaaa gatggtaaaa aatacatgga attgctacct gccacatccg gagaaaacaa
    39721 tgaaagcggg aaaacaaccg aggttagaaa acaaactaca gcgattgaat atgatgcctc
    39781 tgggaaaaaa gtaggggaag gtccgtatat gtttataccc actcagccat acggtgggaa
    39841 gaaaaatggc tgttgggctg agaatggcaa aaaagttcca tatttaatgg gaaaagttca
    39901 gacaggaaaa ctcgctgaaa tgagtgattg taaataaagg cactgaaatc tatccaaaaa
    39961 acaatctacc aagtatgact aggaagcgtt ttctagaaat gcctttttgg actctatttg
    40021 gtagactcac tatttggaaa tggaaaagat tgttctaaat tcaggctatt tctgaattta
    40081 gaatccggat tagtatattt atgataagta ccttgacgaa atatttttat ccttttttat
    40141 ggctaaacta tgagagaaat tattaaatta gcttgtactg gttgcgagac acctggaaaa
    40201 tcagcttatt ttcagacaaa aaataagaaa ttaaaatctg ataaattagt cacaaagaag
    40261 ttttgtaagt tctgtagaaa gcatacagac cacaaagaat ctaaagcata gagatggcag
    40321 ctaaatcatc acaatccaag tcttcctctt cttcaaaagg ctttgaagaa ttgaaagaag
    40381 cccttctaga gaagaaaaac gaattactcg ttaaattaaa cacttgggaa gacaaaagct
    40441 cgccttcagg tctaaaagaa gttggagaca ttgcagacat agcttctgaa atcaatgaag
    40501 aggctttaag ttcagtttta actgaaaacg aaatcgatct actgaatcaa attgaaattg
    40561 ctttagaaaa aattgagaaa ggaacatatg gaatctgcga aggaactaag aagaaaattc
    40621 cttatgcacg tttaaaagca attccttgga ctccttatac tgttgagtat gcagagattg
    40681 cagcaaaaca aaaagcaaaa atcagcacaa gaacatctga tactgcttct tatcctgctc
    40741 aaccaatgga cactgaactt ttagattaat cttttgcaca tctaaaccca aaatgatgat
    40801 attctgggta atttttagga tcgtggtgcc tgcgcttcga tcctctagca tagcttttat
    40861 cccaccacca agaacctccc tttaacactc tagtattata tccagggcat ggctcttttc
    40921 catcacaggg acccttcggg tctttctttg agcaatcagc tccacaaaga gcaaaagatt
    40981 tggtataata gtcatttacc cattcccatg agtttccagc catatcaaat aacccatact
    41041 ttccactcgc tctagttcct acatcagagg taggcaaatc tttatctttc ccgcaaccct
    41101 tctttccgtt ttcctcgatg accgcgcgtg tgcaatctgc cggttcattt ccccatggat
    41161 agatatcccc atttgttccg cgagctgctt tttcccactc cgcttctgta ggaagtcttt
    41221 tacctaagaa ctcacaatat tctttggctg tataccaact cactcctgca atcggctgct
    41281 ttgcacccat ataaggtcga ccgtatcttg gacctatata attacatgtt ttgtttttta
    41341 aacattcctt gcatttccca gcttcaaggc atttattaaa atcctcattt gtaacctcgt
    41401 aggtatcgat gtaaaactca gaaacataga ttacctgttc tggcatttca tctttttcat
    41461 aaacgttact tccccgaata aaattcccag caggaataca tttcatattg gagtaggact
    41521 tagtgcactc ttgtgttgtt tggttttcta cacgttgaca taaactaaaa aataaaatac
    41581 tactaagaaa taaaagaatt ggtttcataa atccatgatt caaagaaagg attttctaga
    41641 cagaatgaat ttttctgaaa tgattcaaaa aggtttttaa tgattctact ctgactttaa
    41701 atcctgttat tggattgact aggatttgat tcaattgaat ctgacttaat gcttaaatgg
    41761 aaatttgttc accgaaactt tttccataaa tgaaataact cactgaatct tgaaaacaca
    41821 taccttcaat cgcacaattg gtctaacaaa cgcaatcatt ctcatgatgg ggaatatgat
    41881 tggaatcgga atttttgttt atccagctct tatttcatct cttttaccac attctctttg
    41941 gtttttattt ttctggtttc taggtggctt gatcgcagta tgcggggctc tgagttccgc
    42001 ggaacttgca agtgtttatc cagaattggg aggcgattat gcctatctta gaaattcatt
    42061 tgggaaaagg tgggcatttc tgtatggttt ttttactttt tttataacct ttcctggatc
    42121 tatcgccttg ggattatcac ttgcagtcca ctaccaagga tctattatat ttggagattg
    42181 ggtaaattca gtttattata cgatacccgc cataggattt gaactgcatt tctatcaact
    42241 catagcaatt gcgattctct tcatcttaac tattgtaaat cattttggaa tagaggcttc
    42301 aattcatttc caaaaggtgg tcacttttct tcctgtcatt tttctggtag gagtagggtt
    42361 attaaccatt ttttcgattt tatattattt ggttttagga aatacttcta ttttgagaat
    42421 aacggataat ttgaatatgc cgctagaatt tccaaatcta cttccttctg gaactgcact
    42481 tgttgccatt tattggacgt atgctggttg gaattcccct ttggcaatgg gtgaagaaat
    42541 tcaaaatcct gagaaagtaa tcccaagaac catgatactc ggaccgattg tagtcatgct
    42601 tatttacttg ttgcttgctt tcatatttat ttgtctttta ccatatgcaa atattcaaag
    42661 tggaacagat ccattttttg cagttgggca atatttttta aagaatctat tgaaattaca
    42721 ttcagaaaca gcgatcgaat ggattccgaa gattatgact tatataatat tttttataat
    42781 tctaggaaat acaaactcaa caatcataac aggcagtaga attcaagtag caatgtcgag
    42841 agataaactt ttctttgaaa agttaggaca tttacatgag aagacaaata cacctgtatt
    42901 aggaatttgg cttcagagta tatgggcgct tgctatgatt ttacttgtga ataaagagag
    42961 caacctatta aacttttctt ttatatgtat tatagcttta tctatactaa caatatattc
    43021 cgttttttta gttaaaaaga aacatcaaaa gcctcattta attccttgga ttagctttcc
    43081 tattgcacct attgtttata ttctagcaac aggattaata ttaatattaa ttctagggaa
    43141 ttattttaga gaaggaaatt tttcaatccc agtctttgca cttctgaccg cgcttatagg
    43201 tctttcttta tatgaaatct ggagaaaatt taaattttaa ttcaatgtgc tcattggtta
    43261 ctcgctgtaa tccgctctat aaattattaa ttttattagc catatttatt tctttttcat
    43321 gcagtatttt ttcacaagaa attagatatc caaatactcc gatctatgaa cgaaaggatt
    43381 tagttcctta taaaatttat aataagcatc aaaagaaatt tagaaaatta gtaaaaaatt
    43441 ttttccattc aatgcctccc atcacacata agggcaaaga ctttactata tatctacttg
    43501 ataagcaaga gatgaatgct cttgaaaaat caaatctacc ttatgaaagt tttacgaaat
    43561 cagctccttt taaatactat gatacaagtt atgactctcg tcttaaaaga aagtttcata
    43621 atttgaatga tttaaattct ggatacaaag atcagcggct aaataagatt tacctacaag
    43681 cgattgcaaa agaatttccg gataaggtaa aatatttcga gataggcaag acaagactag
    43741 gaagagcaat acctgcagtg caaattactt cgcccaagaa agcagaatat aaagtcccaa
    43801 ttttatttac aggagcacat cacgcgaatg aattgatttc tactgaacat tgttatgaca
    43861 ttatttatca tttactagat gattacgata aatatgaacc ttttctagaa actatttcga
    43921 tttgggttgt tccgattgta aatcccgatg ggagtaattt tttttggaat tattccattg
    43981 atatgggtag aaagaatggt tatttaccag acgatatgcg tgaaagtaac cgaactagag
    44041 gtgtcgatct aaaccgcaat tatcctttta aatggaatac aggtcaccga tttgcatcct
    44101 caggagataa tacacatcat ttctatagag gtccgagccc aggttcagaa cccgaaacac
    44161 aagcaatgat gagactcgca gatctagaga gatttgtatt ttctatgagt tttcattcat
    44221 ttgcagccgc gattcttttt ccttacacaa ttgaaaacac tttcaacccg gaaccagact
    44281 atgctaaaac tttgggtcaa cgcttgctaa ggtctgccaa gtcagttaga acaggaaaag
    44341 aatttgcact cagaaaaaat ctttatccag tagatggaac agatcaagac ttctactatc
    44401 atgagtatgg aactatcgct tacatcgctg aatcatcaca tcaaaatgtt gactatgaaa
    44461 ttgtccagca tatcctaaaa ggatttaaag gtgtatggga gaccttactc tatgaatttt
    44521 ttcatggaca taagattgtt ttgaggatat tggataaaga aggaattcca atagaagcaa
    44581 aagtagaatt tcctgagata aaatattttg aagacgagaa gttttcaagt catccacaaa
    44641 ctggaatcta cataaaaatg ttacctgaag aaaaggaata taaaattcat atttcaaaag
    44701 aaggatatga gaaacaagaa ctcgtattaa aagcgggtaa gtcaatgact ccaattcttg
    44761 taaaaatgaa tcctcaatga aaaaaatatc ttatattact gccctcataa tagctagcta
    44821 ctatctgtta ttgattttta gattctgtca tccctatcct aagtcagaat attataatgt
    44881 gcttggaatt caatttttag tagtattcat tgcatactta actaaagaac gttatggaag
    44941 aattatgact gttattgcgg gattttctct agttcgaatc ggaccaatct tgcaaataag
    45001 ttcattaatt acaatcactg gagtcttctt tggagcgtca tgcggtatac tttttagaga
    45061 attagtccta tatttttcat taaaaaaaga tcgtgaatta gaaaagcatt catcctcttt
    45121 tttaaatcct ttcttaatac ttatcttttt tctttatcta ctaacactag aaagatttat
    45181 gatttatttt ggatcaccat ttttaaatgg atttggaatt ttagaaacat tgtatctaaa
    45241 aattctatcc tctaaattag cttactctct tagtatggaa acaataacga atttattttt
    45301 tccgatttta tatttatttt tagaattcac ccgaaacgaa aaaaacaaag taattcaaga
    45361 cttaaaagat ggtgtaaaaa tagttatagc tcttcaattt ttgattatga ctctccagat
    45421 agtttggaat cgtaattttc tagcggataa tacaaatttg tccctagaag caaatagagt
    45481 catgggatta ttcagagatt cgggaagttc tacttggatt ttaccaataa tctgcttttt
    45541 attttacaaa gattttttag aaaagaaaaa atatatattt ttcacgttaa ttctagtatt
    45601 ccaagtatat atcgcatcct ttcagggaag aggattttgg cttttatttt tcctagggtt
    45661 catcttgcaa tttcttcaat acttcaaaaa tgtaagtatc gattctaaga aaaatttctt
    45721 ttatgtattt tcgatctctt tattaacaat tggttttata gcgttaattc ctaaggataa
    45781 aaacgggact ctttatcggt tactttccat tccaaatcat tttttagaaa tggttctatc
    45841 cggtaaaaat ccttttttgg aatttgatcc tcatagatat tacttcaatc ttgccgcttg
    45901 gaatctattt caatcacatc cattatttgg aacaggaata ggtagctata tagtaaattt
    45961 aaaagattca tctctaggtc tatacattcc agaaaataaa attgataatc cttcctttta
    46021 tttaggaatt ctatctgaac tgggcatatt tgggctaatc cttattatcc tacctttctg
    46081 ggaaagaatt cttaataagg aaaatatttc tattttaata ttactaagtg ttggtctaag
    46141 tttcggatat catattgttc agcccgatag cgcttttgct ctcctactta tactaatcat
    46201 agggtgcaaa gaaaattcta cagagttgaa tcataatttt aaaattatct ctatgacaat
    46261 acttctctct ctatacattc tatatttatc ttttcagatt ttatctcaag ataaaatgcc
    46321 tgaatttaga aaatttgcaa ctggacaatt tcaactttta gcatatgaat taaacaaaga
    46381 aacagaaaac aagaaatacc atgtattcaa gggcaaaaca atatggtcaa tcgcaaaggg
    46441 aataggtggg attgagatcg atcccttctt agataactct acatccaaaa aaactttaaa
    46501 acaaagatgg agtattttag atgcaaagcg aaattctcta gcttcttttg agttgacttt
    46561 agagaaagaa aaagaagtgc taaaattaat acgtattccg gataacgcct attatctaca
    46621 agtcgaagag ctagatgaga aaggaaaagt tcaattgtat ggagatattc ctttttgtat
    46681 tccaatcatc catttttcag agatgaatga attcttataa attagattct agtgaaataa
    46741 atatttagga ttgcttttcc ttccggaaaa atgtattcaa ccatgaaaaa tattctactt
    46801 aaaaattgga aaagcaattt gattgtatat accaaaatat atgcaatctt aattagcact
    46861 ttatttatca catgcgaaat agactttgaa agaaattggt gccaagaaaa attagaaaat
    46921 agagagtatg cagcagatcc cgtttttact ctatatacat tttgctctat tcgcgagaag
    46981 aagaacacag agtctcaaga agaatacgat gcatatgtgc gcaataggac aatatcatgc
    47041 aaagaatcag caaatttaca aattttaaat tattacttgg aaaaccaaaa aaaggaagag
    47101 tgtaaaaaaa taaatcgctt acttcctacc attcacgaac ttactatgtg acattatttt
    47161 cgattcctaa cttgtaaaaa acgttatata aaagctttta attaagaatc tttatctttt
    47221 ccttttgcca cagaatctat ccttatctca aaaaaagttt ttctcctaga aagtttttcc
    47281 ttgctaaact aagatacttt gcattcttat tagtaaggag ctaacattga tttttaattt
    47341 attagaaaaa gatatattac ctgatttttt aattcggtta ggaattcgca atttactaaa
    47401 agctagacta gaagaagaga aaaaagaaaa tctagaagag caacaaaaac atttaaacca
    47461 atacatcgaa gatttaaaga aaagtcccat cgcaattaat actgcggacg caaatgaaca
    47521 acactatgaa gttccaacag aattttataa atttgtttta ggcaagagaa tgaaatacag
    47581 tgggggctac tggccattag gcgcatatac tctagatgaa tcagaagaaa gtatgttaaa
    47641 actttcctgc gaaagagcac aattaaaaga tggtctctct cttcttgacc taggttgtgg
    47701 ctggggttct gtatcgctct atgtagcgga acattttcca aaatgtaaaa taacagctgt
    47761 atcaaattct aaaacacaaa aagaatacat cgactctgtc gcaaaagaaa agggctttaa
    47821 aaacctaaca gtgattaccc gcgacatgaa tgaattcaca attgatgaaa aatttgatcg
    47881 tattatatct gtagaaatgc ttgaacatat gaaaaattat caaaagcttt ttgcaaaact
    47941 atcgagtttt ttaaaaccag atggtctttt ctttattcac attttcacac acaaacaata
    48001 tgcatatcat tttgaagtga gagatgaaac agattggatg gcaaagtatt tctttacagg
    48061 tggtatgatg ccatcacaca atttgttttt atactttcag gaaaatttga atatagtaaa
    48121 tcactgggtt gttaatggaa cacattatgg caaaacaagt gaagcatggc ttgctaatat
    48181 ggataaacat aaatcttcca ttcttcctat tttagaaaaa acatatggaa aagaaaatgt
    48241 aactaagtgg tggtcctatt ggagaatttt tttcttggct tgtgcagaac ttttttccta
    48301 tcgaaaaggg gaagaatgga ttgtatctca ttatttattt agaaaaaaat gaaatatcta
    48361 atctggtcta ttctactatt tactctacaa tgcagcataa atagtgcaga tattcgaaaa
    48421 ggcaatcatc tagagaaagc acaagaaaca tctgtagagg aattctttga gcgaagaatg
    48481 aagaatagaa ctaagaaggt aataggagca aactgggaaa ttttttttga atcagaagat
    48541 ttcgtttact atggatatcc acttgtatct ttttttagag aaggaacttt tgtagacgaa
    48601 ttcttcaaag taaatcggac taaactccaa aatgattttc cagctttcca cgaatttgat
    48661 ggaatcaaac ttagaaatct ttgctacgat tctttagcgg aatacagccg ttcaacgaat
    48721 gattcccttt tccaaagttt taattgtaaa gcatttctgg aacaagaatc catagagata
    48781 tttgcagact tgacttttaa gaaagagaat caatataaaa tagaaaaata taaaatcatt
    48841 tttgataaga aaaaactgaa aagagaatca attctaaaat tatagaactc agtctaatat
    48901 tatagatgag caaagctcat ctagtttaaa ggagattatt aaatctatga aaaaaatgct
    48961 cgttgctttt gttgttagtt taatttttac gactacagca aactctgaag taattgataa
    49021 tgcgggtatg ttttcaacat ctacactaac gtccgcaaac caaaaattgg acgaagtaaa
    49081 aagaaaatct ggtaaagaat tagtgattga aacaatcgaa gaagtaggac aagcagatgc
    49141 aaatgcactt gccctagaaa aagcaaaagc tcgcaaggtc aatggagttt atgttttaat
    49201 ttcaaagaaa gatcgtaaat taaaagttga gattggaaat aaaacaaagc ttgtatttgg
    49261 cgaatccgaa agaaatattt taaaagcaaa atttgcagaa gaatttaaac gccaaaattt
    49321 cgaccaagga cttttatcat ccgtcgatta cttttcttct gtaatggatt cggcagttgc
    49381 aagccaaaca aataattata gttatccaca ccggacagac gcgcctatcg cttctagatc
    49441 tagtagcgga ggattctctt ggctctcaat aattatattt gccgtttttg catttattat
    49501 tttcaaacta atttctacat ttctctccaa tagaaatagt gggaatataa atcaacctta
    49561 tggcggtggg tatggttatg gaaattctgg tggtcccgga tttatgggaa cattcttaac
    49621 cggcttatta ggtgcagcag ctggtagctg gctctatgat aaactaactg gccatgatag
    49681 tggtctttat ggcaatgaca attctagcta tgacaactat cgcagtaatg actcaggttg
    49741 gtctcaatcc gatagcgact actcaagtga ctcctcagac tcaggctcag attggggtgg
    49801 aagtgatgga ggagattttg gtgggggaga ctggtaattt aataaaatat tttcttattt
    49861 aaaagtgtaa tttaaaacat acagaaagga ctaataatcc tttctgtata gcttcgcctt
    49921 atcgcatttg tgtgttttta ttttattagg ttagatactc ggaaaattga ttacttttaa
    49981 ctataatggc tttttcgata ctgaaaaaca attgtatagt tcgatctttt ctgggtaaga
    50041 tagaccttag tgatcaaatc aaaatttggg atctctgaaa ttccgactgt cgttaagtgg
    50101 agggtggaag cctccgcaaa atcaacagaa tcggctatta gtaagtatac caaaaaagct
    50161 gagatagaca tactgaacta ctacatattt agtggacacg attttaggca aattgcttct
    50221 catatt
//
LOCUS       DAOQOS010000028        50192 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_264_length_50192_cov_5.850749, whole genome shotgun sequence.
ACCESSION   DAOQOS010000028 DAOQOS010000000
VERSION     DAOQOS010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 50192)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 50192)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50192
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_264_length_50192_cov_5.850749"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(62..2602)
                     /locus_tag="PLF66_08150"
     CDS             complement(62..2602)
                     /locus_tag="PLF66_08150"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HMZ64049.1"
                     /translation="MAQIDYTELKESFVNSSLSILSKLESYVLVLETSIDKNAINELF
                     RGIHTIKGNSGIFQLEKIPALSHAFENVLNLLRNDRLIPNPDLIDLMLLAIDRLKEMI
                     TTLAVSDLVEVSDLVDKFNSYLTTGSDRLITKDAEIIERPNLEEKSEWMEDIKLSEKV
                     ITFAKSKKKYLSIVVVNLSVQSFNFLSEFIEKLKSIQGKVRILRRGLVEKKILPLDTH
                     EHPKVPYYLIILSDNKVENFLKEEGLILEFVSTVVVPENMENVYVRKETEANKPEPEK
                     TPPKQSASPSVDSDVNTSINVNLNLLNNLINLTGEIVLARNVLALKIQQSGNPELEII
                     SKRFEKLLSELESGILKTRLQSLSLFFQKIPRLIRDISKTTGKQIEVAIEGNDVELDK
                     ALIDQLGDPITHIIRNAIDHGIESKEERESKGKTPWGNLRVAARLNGGNVEIIISDDG
                     KGLNTEKIKEKVIQKNLANYETVSKSSNEELVEYLFLPGFSTSNAVTNLSGRGVGLDV
                     VRSNLKKVGGTVSIEFEVEKGTTFILSIPQTLSIVTCLIVKSANRKFAIPQHYLSELI
                     RINPTKIKSIDNKKIYNLRNQFLPFANIGSIFHLEEKSDEKAHYIVSLKSEKFYFGIA
                     IEEVLDREDIVVKPLGEYFEELSFFSGATILGDGDIALILDVSGLARLAGMQAQAKLI
                     ESIPLKEITNQSDSYLLFSIGKSRIAMSIQTNPYIVKVSQKEILNSIGLDRFLYNKTA
                     IPILWLNEILNIEIDIKSIQTIPIILYKHKSGYIGIAATHVEGVVTDLENIQSESIVG
                     EGILGTGYRDGESILLLDTDFILDEFYHKKLKNLKLKIEENKEIRSIV"
     gene            complement(2607..2924)
                     /locus_tag="PLF66_08155"
     CDS             complement(2607..2924)
                     /locus_tag="PLF66_08155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64050.1"
                     /translation="MNFFRKKSKFYIHIESNDMRNSPEDLIGLFVELNSEINGDQQLI
                     IRFIEVEDISMNLLNVVIPFLILMQKTHTYPVSIQAEPSLVRKFKEANLTTIVSRLEN
                     TRE"
     gene            complement(2969..5044)
                     /gene="recG"
                     /locus_tag="PLF66_08160"
     CDS             complement(2969..5044)
                     /gene="recG"
                     /locus_tag="PLF66_08160"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020769638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HMZ64051.1"
                     /translation="MNLKDSIDTLKGIGPKKLGVLKSIGIHTLFDLVYYFPRRYLDRN
                     FSSNILLKNGEIVTLIATVIDSYLAHGRKSRLIVGVKTANNERISLIFFSGVNYFSHV
                     FKPGMNLVVSGKLEYFRGLQIAHPDYEIISGDQDEEGDFTHAGRIIPLYPTTETLKKE
                     GLDSRGFRRLIKQIIDKMEGKSFKIEEVLPEKAVKKRKLLKRQTAFQEIHFPSTDETL
                     VEAKRRFAYEEFYYFNLLMEYKRTERNKIKRVLWPLRKSEGVKKILERLPFELTEDQK
                     ASLEKIKELNGKDVPMAVLLQGDVGSGKTVTALLTAMYYMDNNIQVCMLAPTEVLARQ
                     HFHTIQGLLQFTPFSRMDLFVGKEKEKTRREKLARLKEGETLLVIGTHSLLQEDVVFS
                     DLGLVVIDEQHKFGVEQRETIRSKGKNPDLLAMTATPIPRTLCLTLYGDLQLITIKTK
                     PKGRKPIVTKHFTEDKRQAVYNSIKKYLNQGRQCFIIYPLIEESEKVDLESCIAAYER
                     LRLFEFKDYKIGLLHGKMNAQEKESVMHSFKKNEIQLLVTTTVVEVGIDVPNATVLVI
                     EHSDRFGISQLHQLRGRVGRGEHESFCILLTPDKISEDAVTRIEAMVSTNDGFELAEV
                     DLKLRGPGELLGLRQSGLPDFKVGDLEKDEKLINESREDVLEFGSIGDVEKLEMRSRF
                     TEGRILFPN"
     gene            complement(5098..5448)
                     /locus_tag="PLF66_08165"
     CDS             complement(5098..5448)
                     /locus_tag="PLF66_08165"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HMZ64052.1"
                     /translation="MKAELTEKTNDKIEELKSSLSPIFKKYNLTKVTLFGSFSNGTNS
                     KRSDLDLLAICDTQLSFFERLDGILKEIQLAVPVWGVDLLIYTPEEIEKIKHRTFIRN
                     ILTSGIVIYEPSQN"
     gene            complement(6102..7358)
                     /locus_tag="PLF66_08170"
     CDS             complement(6102..7358)
                     /locus_tag="PLF66_08170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HMZ64053.1"
                     /translation="MKHPNTDLTIFGKQILNITGNVTNSILSGVKDALDKSMNWTAST
                     LENLSDEKFLQIEDLPVYLQEASNKLKQSADVTNKGMEIAIDSTENAFGVATHSLDVA
                     EDYMIRTLYENKVISSILGSSHDDLISFSKIRLLFRYNGEDVNAETIVDLYRKSGFKE
                     AILFVPGLFCDEHLWMKMADEDTGLGDYFLNKGYFPIYIRYNQGAHISVNGKDLHELL
                     KKLFAIEPNLPLHVITYSQGGLVLRSTLYYAKEANASYLPNIKKVFIISSPNGGSYIE
                     KIGFWAGIAMQASPFWFLKLAGFIGNLRSDAIKDLSHGIIREEDWKLGNQLFRYRTEH
                     YFGELDDIDAYEIYSFAGENDNLFQTFFGDGIIEKWSIQALTEKVFQKKSNPESRTLK
                     LLGRNHFQILNAPELIPFIEKGLAAS"
     gene            complement(7360..8064)
                     /locus_tag="PLF66_08175"
     CDS             complement(7360..8064)
                     /locus_tag="PLF66_08175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64054.1"
                     /translation="MEKIKGFVITVMVVITVILIYSLAKTWFYFEKGVELNEEPQTSS
                     EILVRESPKDTLSENSKIIWSNYLYYPTDLVREAGGFGPEEVMNHARNITFVNLNTGK
                     TKKLFNKKVYILDYFPADFIKKIKKNDEVIKESLDLGNRMLVFAMTVDTNKDGFLNNK
                     DLIQIFLYHPHDEKLDEVLPEGYFFERLLLNSHKNQLVCIIKQKPDPKVKNQNLESMI
                     YTYDVITKKGTIIHGE"
     gene            complement(8051..9172)
                     /locus_tag="PLF66_08180"
     CDS             complement(8051..9172)
                     /locus_tag="PLF66_08180"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000538751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HMZ64055.1"
                     /translation="MGVPFIDIKRFEDGFLDSWNRKVSEMSKNAQFIGGAEVATLEKR
                     LAEYTQTGFAVSCANGTDALQLALRAVGVGLGDNVLLPDSTFWATFEAVVNVGANPYT
                     VDTNLTDLQMDFAAFEKAIDEVKPKAAMIVHLYGWGSSRLSDFRELCKKKGVVLIEDG
                     AQSFGVKYKGEPVYKGCTISTTSFYPAKVLGAAGDGGAVFVNDQALADKVRMLANHGR
                     TTHYGHGAVGWNSRMDSLQAAFVNLSLDHFEARLKSRQEIAARYHKDLKALGVNDITP
                     PSDYVENGYCNVTLFENAKRTKLEAILKEKGIGFGNIYPGAMSDQPGAKEFLKKRFGG
                     ENARQVSSTVLNFPLFPYMTDAEYKEIISIVESFLSGKN"
     gene            complement(9253..9675)
                     /locus_tag="PLF66_08185"
     CDS             complement(9253..9675)
                     /locus_tag="PLF66_08185"
                     /inference="COORDINATES: protein motif:HMM:TIGR04256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HMZ64056.1"
                     /translation="MNQRRLPPMSTEDTDKRYDRVLEKDLTDRIIGACMEVSNTLGVG
                     FLESVYDRALLIELDRMNINYKSQAKLSVVYKGINVGDFIADVFVEEKVILELKVTNS
                     IVKEMQAQLMNYLKASGIKIGYLINFGNPKLEWKRIVL"
     gene            complement(9726..12098)
                     /gene="lon"
                     /locus_tag="PLF66_08190"
     CDS             complement(9726..12098)
                     /gene="lon"
                     /locus_tag="PLF66_08190"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002999831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HMZ64057.1"
                     /translation="MKKAVSKKNIRTKKTSDPDTIEEILPDELHIIPIKNRPIFPGII
                     TPLIVPVGKFSNSVDEVYKNNGFIGLSLLIKEESEKNPVNNIYTMGVIAKVLKKMNLP
                     DGGTHILINTIHRFKITKILKEDPYLVAKVDYPVDKVNTGSKIDLKAMMRNLLVLTKE
                     LAQNNPLFTEDMKLSLVNMHEPGRMADFVASILNLDKEEFQFILEEEDVFQRLEKVII
                     YLKKELELIAVQRKINDQINSKMDKQQRQFFLREQLKAIQGELGMGEEKGEKKYDLLL
                     DRLKKAEVSEEIYAEVKREIERIQVTDYNSPEYNVTRNYLEIIDSLPWEAPPKRNIDI
                     QYAKKILDRDHYRLTDVKDRILEFLAVKKLNPDNKSGTILCLVGPPGVGKTSIAKSVA
                     EALGRKFYRFSLGGVRDEAEVKGHRRTYVGALPGKILSALRILKEKDPVILLDEIDKI
                     KTGYSGDPAAALLEVLDPEQNVNFRDHYLDLPFDLSKVLFIATANTLDTIPRVLVDRM
                     DVIRLSGYITEEKIEIFKKYLWKKVLKRNGLGKEKVTLTSPAIQSLINSYSREAGLRN
                     LERMSDKIARKIAYKIVSKTKVNHSITPDQLEEYLGVPLYVDERMTKADKPGMALGLA
                     WTAVGGATLLVEAIFLKGKEGLMLTGKLGKTMNESTNIAYSYVRNLIDPDGKIFSDKR
                     IHIHVPDGATPKDGPSAGITMAAAIYSLAKDKVVRDGFGMTGELTLTGEVLAIGGLKE
                     KIVAAKRVGVTKIIYPKDNDAHLKEIPDYVKKGVTFYPVSHFKEVEKLLF"
     gene            12145..13302
                     /locus_tag="PLF66_08195"
     CDS             12145..13302
                     /locus_tag="PLF66_08195"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HMZ64058.1"
                     /translation="MKNKHKFLLILSILVNLLLVLFLFPANQVRVKFFFYKLFGQQVP
                     DKIPLFSKLVLIQDYKPISVLPSPDTRSSKLPSMPIIETHGHIGNFFQTKPDDVSRDL
                     TALGIKKFINLSFTTGQEFVQLKKDYADPRIIHFSTFNWKRLSEDNFAEKMLSDLKQD
                     IANGSRGIKLWKNYGLTLKKKNGERLKIDDPELDPLFEECAKSGLIISIHTVDPIAFF
                     SPIDEKNERYEELLRHPEWSFSSSEFPSIETVLQERNNRFKRHPNLKFVALHFAEHAN
                     ELEKAEKFLLEHPNVMVDIAARIDELGRQPIHAKAFLEKYADRILFGVDGPPDRGKLE
                     IYSRFLETTDEYFDYFPENKPRKGFWKIYGLGLSREALEKIYFANAEKLYK"
     gene            complement(13945..14316)
                     /locus_tag="PLF66_08200"
     CDS             complement(13945..14316)
                     /locus_tag="PLF66_08200"
                     /inference="COORDINATES: protein motif:HMM:TIGR04256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HMZ64059.1"
                     /translation="MEFENISFQVIKCAIEVHKNLGPGLLESTYKQCLIYELLENNII
                     FESEKDLPILYKGNRIEAAYRIDLLIEGSLIVELKSISEIKPIHEAQIMTYMKLSQIN
                     KGLLINFNVKKLKFGLRRYVL"
     assembly_gap    14781..14880
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            15955..16857
                     /locus_tag="PLF66_08205"
     CDS             15955..16857
                     /locus_tag="PLF66_08205"
                     /inference="COORDINATES: protein motif:HMM:NF022831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpL1"
                     /protein_id="HMZ64060.1"
                     /translation="MIKMISKLLVVLSLCFFVTSSIFADDQDFKNKGKSRIIVGLGIQ
                     FDSNSLGGTIVKDGLDSGQIKTDSNGNYAGQQKAVIAENKLQTLENLSGGLISYKSTG
                     PMTAGSLTLGYEKDVGDNFFWRAGINFSTKISGGRTSSTASGYDWYDVTWYYKSLVIP
                     VYFGIKLNVGSRSAIYVAPGLHYYQAEWQLKGRNDGNGLDAVTGGLARSLPVASDAAR
                     PGVINEDTKFSGSGFGMSWLTGVQTKVTEKGYAFIEVENHFSYKQGNGGTKSSGGIAA
                     IAPQPAYPVTVGGTIYRVGYKHEL"
     gene            16876..18576
                     /locus_tag="PLF66_08210"
     CDS             16876..18576
                     /locus_tag="PLF66_08210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64061.1"
                     /translation="MKKTFKFLSSVLFVSILAVTNCHYSTGGSQTNGAVVANYVNSLV
                     RSAVTGNCAISVNLGSLYAGAIVQGAAGSATKFPQAKYEAVTGSTISAQNYASYAAVP
                     YNKKYDAFLTNSTDGTSWTNALRGTAIANQKASIDLGAYLGRAAFLCNANAATTGATL
                     AAALTAYRTASTTAGADNFTAAENTAVTTLLTALGTNFATIEGAFTGFGCAAITGALA
                     ATYTNFGGSAQTVSLYNARLAYTSGLALMACARIPRSSCSTSALVTTTRDADVASLVA
                     TYQTINATPECRKPSTGLLASVLSTGATGLPKETTFLGANTTIDLGGGRIYQNTAAVG
                     GSEGSLTGLSSTGLLAARAYPKSGALLGIASSFTSAHPMIAGTTAYPTATDGSVATPY
                     YGGSNINATTVTSCDALGFGNYGPTPIQTTDSLATVNSKKTLTDVKEIVYAFSANNTV
                     AKAYETAIASVSPTTATNDAIACNRQMRKTTTISSALGVGTTLPDINAGSGDGGATSL
                     VSACVYGSNVTNRTVAAAVFSSLLPGLTSCPQAAVSGASSFGVTGLTTFSAASFPDGQ
                     "
     gene            18728..18904
                     /locus_tag="PLF66_08215"
     CDS             18728..18904
                     /locus_tag="PLF66_08215"
                     /inference="COORDINATES: protein motif:HMM:NF025088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4160 domain-containing protein"
                     /protein_id="HMZ64062.1"
                     /translation="MPLVSEFYGIKIYMYWDDHAPPHFHAEYDEYKILVSIKDSTVIK
                     GAFPSKQLKLVSLV"
     gene            19000..19281
                     /locus_tag="PLF66_08220"
     CDS             19000..19281
                     /locus_tag="PLF66_08220"
                     /inference="COORDINATES: protein motif:HMM:NF021873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
                     /protein_id="HMZ64063.1"
                     /translation="MIHEIVQVIPKPNFKVYLYFSDGKIKLFNAAEIIKKGIFKQLND
                     LDIFIGKCTVLNHTLAWDIKGNYDPYNCLDLDPEVLYQNSDDVKDPLTK"
     gene            19329..19853
                     /locus_tag="PLF66_08225"
     CDS             19329..19853
                     /locus_tag="PLF66_08225"
                     /inference="COORDINATES: protein motif:HMM:NF017496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HMZ64064.1"
                     /translation="MTLLAEKKILEYEDIDSLPDGFYEIIDGERKDMSPSGFLHGLIE
                     GSLSEILKENLSKTGSVATGEIGILIQKNPLRVRGADIVYITKESYPKPPIGILEKAP
                     ELAVEILSPANTTTEMNGKLKDYLAIGIPHIVYVDPENEMTTVYENNSFQVFSFEEKF
                     RLFDFEIKMSDLLV"
     gene            19857..21050
                     /locus_tag="PLF66_08230"
     CDS             19857..21050
                     /locus_tag="PLF66_08230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64065.1"
                     /translation="MDKKTIFLLPLDLKGIKQTRITPKFIREIYDPDKANKLPEHIEK
                     KKKNIFTAELNRLIGKISEKNKAEKLQELETKIDPNPTLEEMEEAVALIENEEKEKEE
                     VEVLPQEMETLPKSESLDLLNQIYLGQNAKENALKYLEAMFQTLGIKAYCVFFYEPHS
                     YTYFPVFSNGLTEKAQTNLLFYANDRYIQNRTEGYTHLFFQPVLVNDIFFKKKLSPIE
                     FNQFSSIILKFLDKQNLNGLIALFVDKYKAISEEEIDTLCNSIDKNIEPLVPFLNSYF
                     ESEKKERLDSFDFMRQVIHGIQIQSKNLGDEFYFTKLKITNFLSVEDAVNKKKQLIHL
                     LKKHLLEKEGIIDFSHNEILLLTKENEGGKIIQYISENAQTDFQFEIFPLRYPDNGKN
                     LYLYF"
     gene            21022..21627
                     /locus_tag="PLF66_08235"
     CDS             21022..21627
                     /locus_tag="PLF66_08235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64066.1"
                     /translation="MVKIYIFIFSLSLVASIQSESISRKELSILRENCLKTYKEKKYS
                     EAIPLLEKYLSFREEIKFKTLLAKSILFRTDLIAPKEEDEIFIRDEKIHTAIENYKKS
                     ARVFSEVIPYLEKVTPNDKSIGELYFLWALSEQFSNEREKAIPLYKKSALLKKDLAAL
                     AFYNIGSIYEFLGQKKDSEIYFSKYNQASVDKKEEIREESR"
     gene            21611..21808
                     /locus_tag="PLF66_08240"
     CDS             21611..21808
                     /locus_tag="PLF66_08240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HMZ64067.1"
                     /translation="MKKADKKFQMLFTEDELSNLKEEAAKRKLTMSEYIRMCIKNDLT
                     KKTSYSKIMALHTLKNLNSTT"
     gene            21805..22212
                     /locus_tag="PLF66_08245"
     CDS             21805..22212
                     /locus_tag="PLF66_08245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64068.1"
                     /translation="MKKILITEKVLEELLYGSKKNKTLIANQFEDHLKKNDSFFVSIL
                     TVEEILKKEESIENRKIIFKNINELCDEIIEIKLDDSSSALRIETEYSTDHRTAMELT
                     VASRKGIDCIYDTTDRFDFQKMISVIRLILKGE"
     gene            complement(22180..23811)
                     /locus_tag="PLF66_08250"
     CDS             complement(22180..23811)
                     /locus_tag="PLF66_08250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64069.1"
                     /translation="MNSKFLNITFRIFLFIFSYSLFAGEFIFDDKEDFSKLKKKGIRI
                     VKASAKNFKLELSPKKSDANAELLFNFENKKASELKDATGNYTVNFSSYDVVENKTLV
                     GRRYASFAASNSYISVGTNNGRLLNSTHFTEPFYFSFLVMPGESEQYSVVFTKNLITG
                     GKKFGIECDIVNNKIEVQFHNMFSYSKTESKSLFLKSPDKLKAEEWTHVLIAIDPMSG
                     KAILYEDGKAKSEIEAIMSPENPTSLPFGFHPNDTTPLLIGKGFFGKLDQFLIGLGSP
                     DIERLTEPYRGVQYDDMIKFASQAKGVAYSPVLQTKNSFSRLLSLDFDSNKPAGTHLE
                     IHFRVSNTFFTEDDTDIKWMDSSSFKDAMEIDFKYIQWKLIMRSDYSGKHSPALNKLQ
                     IKYAESLPPSSPVGLKVAAMENSLGVCLSWISNHEENVRTGGKYLIHYGVSPDRMVGT
                     ILINEKSESITGLEDGKELVSSYKTLKQCVDNDLITLNSLNRKDKNLLFFKPGLTYYF
                     KLTACNKNYNDTTGRDQKSIPSNPVSFTFKNQPNH"
     gene            complement(23808..24242)
                     /gene="rpiB"
                     /locus_tag="PLF66_08255"
     CDS             complement(23808..24242)
                     /gene="rpiB"
                     /locus_tag="PLF66_08255"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HMZ64070.1"
                     /translation="MKEKIGIVSDHGGFELKEFLRKQFDSDLEIIDCGVHSEASVDYP
                     VVIREACSRLKKGEFEKLIALCGTGIGASIAANRIKGIRAALCHDEFTAEMSKRHNNA
                     NVIVLGGRVLGKDLSVRIVKKWLESKFEAGRHENRINLLDLE"
     gene            complement(24306..25790)
                     /locus_tag="PLF66_08260"
     CDS             complement(24306..25790)
                     /locus_tag="PLF66_08260"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HMZ64071.1"
                     /translation="MMRLSLKDAVKRVIDTNASVQNAKFEILKADTPIYKNESKFNWV
                     LIAEASKQEFVLPFNLNNFFGGTKGQADRLSAGIEKQFKTGTYFSTEISTLRYDTNAL
                     ENPITTPSSFAFLGVKPLYTGAITFKLSQELLKYSFGRTEKTKEQALRTQAAIQQDSL
                     IFQLTQLVTQTLVEYWSLSVADSAVTTFEKLLANSKNIRNLTARKQAIGIAERFEESQ
                     WNAIVASAESQVERSKLERNDAKRRLQRILGVDPSVEISGVTDLVDTMPPIDLEKDIE
                     YAYENRIDLKNLKRQKDLARLGLSVAEDEDMPSLKISAAASSRAQSLYSPQENFNYMN
                     QNGIRYFKYPEYRAELTMSYPLWDKGVKTSIRDAQVSVKQITDQEEAFRKEVEDELRT
                     RYDAIKSSYSVLLTAKQTEEETKKFYDGLVSKFLQGRYSALAVKNALDAYTQAELAQF
                     QAKVNFNINLIRYDLAKNYLFEKYEIDVYKIVDELKKAAAERMN"
     gene            complement(25986..26981)
                     /locus_tag="PLF66_08265"
     CDS             complement(25986..26981)
                     /locus_tag="PLF66_08265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64072.1"
                     /translation="MENKFSSKKKFIFLLSAFFLTLQLASQDVANKIHPPPKLQTDLF
                     GRSYFLKWDGAIEFSVDDSYKEAMPDSSQLEIHMGEAKALIEKRKVFPAIRLLKGLAL
                     CERMRNQSKKVFNPEAQKTLNKLLSQEQDKREELEILTEPYGCYTKNQKGIRKLFIES
                     KDFGFFYELDERFKYVFPTDLYRFARKEKEYNWKVSYFRIFLPQAKKEESFETEYFRY
                     ENQIFYESPAKIVLSIGQTYHIHSQNKEMYPFVWDARRGLSESVKKGTNFIRKEFQSG
                     YETSFYLTENHEIKSFVGFEKYRFHKNKGLGIFLVCPSEWKEDCNKIWREVESQK"
     gene            complement(26993..28549)
                     /locus_tag="PLF66_08270"
     CDS             complement(26993..28549)
                     /locus_tag="PLF66_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010419320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64073.1"
                     /translation="MEEIESQEENSEREVRAKDKKRIAICSSSLGREGRALVRGQVVD
                     VGITDIMEADDLWNLMTGLFQGQENEITPFLDFSLAPVRKPVLHLEIYSEKGKKVYET
                     PDFVGTEDGFFSYELTHKLKAGNYTFHVIFKGSDSYRQYTKDIAYLNLKEHSKITKVT
                     IVGMGKLRILSEKYNSFITTSDIDQTYLATELGSKKGMFSTLFETPDQKIPLPAMPLF
                     YKKLREDTKGSPLAFISASPHYFRRALHATIRNHGIEMESLHLKYLDGTIKGVVDKVV
                     NSVLNLNDLLKEGVGPAVDRAKKFFGSTYQSLFDQLTYKLTILLQDRLYQPTKAKEIL
                     LGDNTESDYLIFTLYQLILLGVISGKELEDYLYHINFLGRDAVTRDNAIKIRKLGDEC
                     HAIHGKVNSVHAVLINMTNIGPMAIDMEENVVKALPKGVDLKKIKSFKMYIPTEGALG
                     FATNLVGLGVMEFQSVIDILMDMTGKWLNGKVLDDKFLISLSRHLSVIPKAEEHRQML
                     YEIVTEALAE"
     gene            complement(28838..30133)
                     /locus_tag="PLF66_08275"
     CDS             complement(28838..30133)
                     /locus_tag="PLF66_08275"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HMZ64074.1"
                     /translation="MLGSNLAKLPLGVNLTTREPYNVFIVEDSVLFRTALKRTLAKLS
                     FSVIGEAGDGLHALNQLKEMHIKPDIICVDQEMPVMNGMETIKEVKNLYPKMKIMLIT
                     SHNEGAFVKQVLQVGVHGYIVKPFETDTIIRKFSVILGRRDILGSFDEKIEKIDLSKI
                     RLPNLPLVFASVVNFDVDDPKNGVAELEKIISPDIAMTSTIIRSANSAYYGRSGSIRN
                     LKDAITLIGTKITRRMVINEFDKTLTNPLKDPVFVKYLRELPVLTSLISYDLLTPLNL
                     KTLAADIFVVSLLRKIGMNILALNFVEKYLKVLRLYEFGVKSLYDLERDEIGIDSIEI
                     GARAFEIWKMPSHFVKVIAHQGFNKEELEVVSDIDRVSRLAEILAKQMRKIAVTDREK
                     ELAPLILQYHKAPANTIDLFGEDYLNMIEDHPFMRIANG"
     gene            complement(30183..32135)
                     /locus_tag="PLF66_08280"
     CDS             complement(30183..32135)
                     /locus_tag="PLF66_08280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64075.1"
                     /translation="MKNIINKFPKKNISKIILVLIFFLCSAALFSQAANRKKPYLVKN
                     TAPSSSLMIEVSGPKILKEYIGYKDITEDITSEISVQVKYDDTYRAKSIEVQRYTITG
                     FSQKEYSAFRIMAHFGENLKENINNYDEFLKVLYKPAVNGDYSLYVIDDHKFKLMKSF
                     KSKDLVGGKFHLDSALRFTKEGGAGVTVDKGIIALIPDSEILSIEKHSTPENLPIYTI
                     SVTGEPFDSWFEEQNISPDSHFEMTGTPPHIVNYTDGKKLTVAWMESNGTKVHIQEFN
                     ESFEPLDEIVYNTNFPIFGGFTKDEKGNYFLVVAKNNLDGIFSPNIKLVKLDGRGKEV
                     KSFSPLINREDFDVMKPIDAGTSRVVYGNGKVAVHLGKTQHKNKRDGLNHQSGILFVV
                     NSETMEFLSGESQTWTASHSFDQRMIFDGKDFVNLDLADAFPRGIQITKKKTGRLIFT
                     YKAGKMYQKTFTELGGLVSVSDGYLVLGASEKDFNPSNAEIYLNDSRNLFLLKVAKNF
                     NEVKVDSKSQYLISKDIVISDGENSPEISFVDYAGNKYPQKRVGVQWLTNFKKKESEN
                     VLRPKLIQLEEDRFLAVYEKWSANSYIRTEYIIFNSSGKILQEPVDLGSARLNRKDDP
                     VYINGNVVWTAGKKDRRELKVFVLKP"
     gene            32293..32553
                     /locus_tag="PLF66_08285"
     CDS             32293..32553
                     /locus_tag="PLF66_08285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64076.1"
                     /translation="MNVRDLYNIYESINRDIAKLKTTRSSIPVDREYTTILQSTITSE
                     IEKFESLKKLIQELEVEIPDDFKTIEKQSVDYSETTFFPPML"
     gene            32569..32901
                     /locus_tag="PLF66_08290"
     CDS             32569..32901
                     /locus_tag="PLF66_08290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64077.1"
                     /translation="MKKQLPDNLKKKLKNEEDKNWSDQQINFILETHNAKIVEITGYK
                     PHADYINKTWEFGKARPEYREICESCQFPYAFYAEPRNWSMGSDSDVWNLMKCANVDC
                     GKYFESCL"
     gene            33951..34112
                     /locus_tag="PLF66_08295"
     CDS             33951..34112
                     /locus_tag="PLF66_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64078.1"
                     /translation="MKKESYKKKKLPNKNDKVVNLNLFRIKKSLTEEGFEVIENEKGE
                     IKLILRLAK"
     gene            34303..34375
                     /locus_tag="PLF66_08300"
     tRNA            34303..34375
                     /locus_tag="PLF66_08300"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:34336..34338,aa:Thr,seq:cgt)
     gene            34390..34854
                     /gene="ribE"
                     /locus_tag="PLF66_08305"
     CDS             34390..34854
                     /gene="ribE"
                     /locus_tag="PLF66_08305"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: protein motif:HMM:NF013081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HMZ64079.1"
                     /translation="MPYKEMIGSLNGSGQKHAIVVSRFNEFITESLLKGALDSLKRHG
                     VTEEDITVIRVPGAYEIPFTANALAKTKKHDAVICIGAVIRGSTSHYDFVAGESAKVG
                     NIAIENSVPVIFGIITTENIEQAIERAGTKAGNKGFEAATTAIEMVNLLKQI"
     gene            34854..35276
                     /gene="nusB"
                     /locus_tag="PLF66_08310"
     CDS             34854..35276
                     /gene="nusB"
                     /locus_tag="PLF66_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010576239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HMZ64080.1"
                     /translation="MASRHKSRSIALQALYQIELTGVSIGDALSFSWYDKVLEEEEKE
                     LATQLIKGVVKNWDLLDTIIKSYSKNWDFERISVVNRCILRLSIYSLINQRDIPPKVV
                     INEAIELTREFESETAVSFINGILDAVYKDELAKLSGD"
     gene            35279..37348
                     /locus_tag="PLF66_08315"
     CDS             35279..37348
                     /locus_tag="PLF66_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004783862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HMZ64081.1"
                     /translation="MEPIKKNRLKFESVEPETNPGITQYETDEYSTMQLRQRTSTPLP
                     WILLFLVLIIALGGASWYYFFGRNGAEGFSKGDLADDKTGSLDRILERPYMPASSTNP
                     RLAECINLYLEKYQKKAETKCEEFLNRPESDQDKSIALTVLGVIHDEKGRYAQAIEYL
                     RKAIAYDSKNIYAYYDLAIAYKHDGRMSDARTTIQKAKDLAPRDEKVSLLAGNLLYDS
                     SDPKAAIEAYKDGLSSSPDDPSLVYNLALAQYKQGMIPEAIENFRKAIQSGGSGQIAE
                     LAHGHLGSIFYYREDLNGAEHHFRQAVTLRPNDARYLYNLGIVLIKKKNTEEAVSFLQ
                     KALDAGATDPSIYRYIAESFEDLKMHDNAIASLEKAMRLRPDDIDTLVQLADLYYNRG
                     NLSQAEETFRKVIRITHGDSNTESARVNLGIILDDMERYGEAIFEFEKAIELNPKNEN
                     AYYNLGIAYKHAGQPTKALESWRKAYAINPNENKYKEAIGDYYLESNYLDEAAREYED
                     IAKANQYNYKVRLKLADAYFRKQQYDLAEKNLIFVLNQSKNAEEIKLAHRKLALVYSA
                     GDSKNKTKAKDEAYRGSHIDPDDMEGRLVLAKVLIDSNSLMDREKAIDELTAIIRSDV
                     KPKLAAKAYNYLGVCLYKNKEYKRALQEFQNAIDLDPNLTEAYDNKRAARAEYEANLQ
                     SKQSSIY"
     gene            37401..38843
                     /locus_tag="PLF66_08320"
     CDS             37401..38843
                     /locus_tag="PLF66_08320"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HMZ64082.1"
                     /translation="MDELNLDSMKEQTTVKKTSFWAPKQSWIFILLTLSLSFGIFATS
                     EKDELKENLLGSEKKQKQALLTIKNANREDLFPIVSDVLRKTSSEGEIQGLILDLYFS
                     YGPEIEKYNPKIIEDLEWVFENTKNETNLIKVLHYAATYKEKRLMYHILDFIRYRDAT
                     VREFTFKAIDSFKDDRALPFILELGSSDQPILRYYYLESLNYINDERASMHVAKLLTD
                     PSPAIRSESIVVIEKLGLKEKLNSVLAMATNDSNYEVRKTAVVSLKNQRSKFPTTVYQ
                     KTIFDSNREVRDVTVDAITTIKDPSYAKFISAAMEKENLSPLRLKMVDSLLVLNNHGG
                     GFGLSAALKNDKDSEVRAKAALAIGKLKATPVVRDLVSSLDQEKVVNVKIEITRSLGS
                     LKEKSAVPVILKKLQTNEANELRSEFLTALNQIDDPKVMPVIFDLIDLENTEIIKTGM
                     KSLLRTMLYRYHGGGKEYRTLILSDKISMN"
     gene            complement(38840..40546)
                     /locus_tag="PLF66_08325"
     CDS             complement(38840..40546)
                     /locus_tag="PLF66_08325"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HMZ64083.1"
                     /translation="MLNKLFTNEFLQDTDVQDFKKGEKLIEEGAPSNETMYFILNGSC
                     AVYKQQGDTPVLINQLKTGDFFGEIALISNQPRTATVIVDSDSAKLLLFSKKKFIQQT
                     VSNPVLMFSILKAAIARFLRADANFENTIKAIPNMKPDLIIKLNQNHVKLVNIKALNY
                     IHSLPILNLGKNLKIFTEGKDANPYMYFILRGSISLLKEHHGKNVELATLELGHFFGE
                     TSFISDTHYSLSAITKSDDVKLVQIDKVAFLKIVNLHPEFLFNNLKNTIWKLINTEKA
                     TFNLKSKMIKEAPASTDVKVYQNKEFIIKEGDPSNETMYFILEGSFSVLKNRGDSVEE
                     VNVLKSGDFFGELSLISSQPRTASIAVKSETGKLLLFSKEKFIQKTKESPGLMFSILK
                     ATIARLIRAESAIDKIIKALPELDPALASRLNDSRVDNINVFNYVHNLTSRVLIRGDK
                     VYSEGEKSDGAMYFIYEGTIAVIKKYHKKNVKITSYEKGDFFGETSIVSEAPRSQTMI
                     TVSEKAKVVRIDKDILMKIVHISPGFLFSLLRTVIWKLIIAEKALFNLNMKVDDIYEK
                     KS"
     gene            40707..42200
                     /locus_tag="PLF66_08330"
     CDS             40707..42200
                     /locus_tag="PLF66_08330"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HMZ64084.1"
                     /translation="MIQAIKNLELKSFFESSFNKLKNRDLAIQLADVQKRFQDLTLFQ
                     SFLQFFSNFTLMLKLVSRSSASVNQNMKQIVSETASLNKELSAIRNIMDDYLEKIHQF
                     SEEMGNITSEVSEIHFQTRNMAEKNNNILESSNDIQRKVQNGAKTMDAGVYLISELVE
                     QNKELNETIRDLWSKYTFLIAHSKELVKISESTKMLALNAEIESAHAGEAGKGFAIVA
                     QEMGRLAKKNSDYSREIAKGIQDMQEQAKITEINVSSSVELAQNSEKEIQEAHKTYKN
                     VSDSITGVLKDSTAFLNSFTHLETSMKLIKENFNSANLLLSESNKKVTEISKAIDSQI
                     NTIEKIDSRTISTFTASRVLNSLISQFTIPGFKDNNPKVKTIEKLIESILNIRGILIT
                     LLFDETKSFTDDLKKEKIEMDSIINEEIKMLQSLLSSELDKNKFSELSLWWKEFQRQT
                     DRILEKLSQFESGSAKEIYTSIKDQISPTLTILVSFIVQDIIDLNDK"
     gene            42206..42664
                     /locus_tag="PLF66_08335"
     CDS             42206..42664
                     /locus_tag="PLF66_08335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64085.1"
                     /translation="MLITRLCKIFNENSLKYAIAGGFAVALHGAVRGTIDVDIVLDLN
                     EKNLTLCESILKSMGFVSKIPIDPAILLKNREMLMQEKNLIAWSFYNPKDPSEVIDIL
                     IHKDLADIDSTVMRYADIELSVIAKHDLIAMKKESGRPQDLEDVKALESI"
     gene            42661..42843
                     /locus_tag="PLF66_08340"
     CDS             42661..42843
                     /locus_tag="PLF66_08340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64086.1"
                     /translation="MRRPVQYFSEEYLDLCKKLSVEQIVQFVEGFRLLFGETANQPKL
                     KQQEKIYLELDITDKK"
     gene            42913..44202
                     /locus_tag="PLF66_08345"
     CDS             42913..44202
                     /locus_tag="PLF66_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010571593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64087.1"
                     /translation="MSVKSFFILFIVCFFSELIAVTIETNTGKVFESVQIMEETESII
                     EFSDSHGVITRIRRSSIKKISEDKPQETLEENADESLPTPMPIQPKKSEVPEHSFLLN
                     QSISNDGAFQGASLFGERQSRRNNQSYKSFSQAWFLTSSVELLGLPKQFQISFTTMNP
                     LVDRTNTDSDLYFQSTPSGANQNKLVSNSINSGTLQYDPNQVKPRKEKNALTDYLFTR
                     LNYEHTTKLGSFGVGFIFINVNDPAYVMRGYFTVSWKPPVLQFLNPVLSINNKMLSEY
                     GGIFQGDHNYRLSIGHEFFKERLIHVTPSLVVGYQDVNNNQNMKKGISDISPRLQFNI
                     SNFYVALNYMYRATPALADTKNYTPDIGVYPDSNQNDGKTIDPSKAYGYKNNFITDSI
                     AANSPNDLVRHVLTDRYQQQHIVRGIFFFNIGYTVRI"
     gene            complement(44233..44799)
                     /locus_tag="PLF66_08350"
     CDS             complement(44233..44799)
                     /locus_tag="PLF66_08350"
                     /inference="COORDINATES: protein motif:HMM:NF019339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HMZ64088.1"
                     /translation="MFRIFFSALLYVFQLFLFQLPLHAQDTTNSHIKQLIQTRQLAKA
                     REELNIAMQGNENDPTLNLYQTELWIIEGETYYNSGQYKKAMEIYQKAVDRYPSNPMV
                     KMKYQEMIKKVKIAEERPKENTPSTETVNEQTINQQSTQQATSEYISLSEMISSKSDT
                     SNLLLGLLCLENFVLIVFVFLKFPKKKE"
     gene            complement(44809..46167)
                     /locus_tag="PLF66_08355"
     CDS             complement(44809..46167)
                     /locus_tag="PLF66_08355"
                     /inference="COORDINATES: protein motif:HMM:NF013507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64089.1"
                     /translation="MKLFLIIILIFSCSENPIQKGNESDKLKVNYSFVRSATETTVDL
                     VWECSSASYGILTYGNIGFENTQILFFKQKIQYYKLVNLASGKTHNYQVGCFDERKAV
                     YIPQKFTTKSVPIPQVVMNRGIWILGGVGSTNTPVSQIDLYDPVTDTWYSSVTIVPTP
                     RSYSGIATLSNKIYVIGGMLIDQSGNTTISNVVEEYSPYTNSWKTLSSIPVAIQGALA
                     SPSGNSIYLIGGSISNSSASAIPNIVYKFTPDSGNGSWATITSISSIAARSDLSGCAI
                     DGTIFFNSGRDTTGTTQFTSDAYIPSANSTTGITEGNFSLARFGTSSTCYRPKSTDAY
                     PSDSPGIFLAGGTTLSNTVQPPTALLASNRFDYYLTPSALNTVQTGPSLPVSVYAPAA
                     EVSYDLRRLYVFGGATIVNVPTSTVYWIDLSNPSTGPWNTVTTTTMPVPRFGHTAILI
                     NR"
     gene            complement(46164..47033)
                     /locus_tag="PLF66_08360"
     CDS             complement(46164..47033)
                     /locus_tag="PLF66_08360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64090.1"
                     /translation="MIKRILFLLLFVQPMYSLELILKSGDAFICDTVEENDRIIKIRF
                     KDKLYEIPKSEIQMRDNSKTGTHKGYTLATITMLDATVVKGNIAEESDTILTVQTILG
                     YLSLDKAKIADVKKPDPKIEVTIPKDYLEQSYRLTPIRFGVNTYFASNGQPLSAISPT
                     IYGGSVFLEPSNLSWKDTWNIGFKSEYLTSSGEGSRYGFYNQIVYLQYRKSFSKWLDF
                     YANIGFGGAYIRYRKDEAYIEGFQGLSATSFGYQGIKFGKLTLRIGPRVLQYFQKPYY
                     HNVGFEIGIVYSL"
     gene            complement(47039..48229)
                     /locus_tag="PLF66_08365"
     CDS             complement(47039..48229)
                     /locus_tag="PLF66_08365"
                     /inference="COORDINATES: protein motif:HMM:NF013595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HMZ64091.1"
                     /translation="MNPFYKTTNILIILTSLAACTKSSNSKDKNILVAGLLFLQSKTT
                     SSTGIGGPTATRVYGQAGSFTTATSNKGGISADSLYNPEGITLDSSGGVYIADRFNNR
                     VLYYPTGSTTATRVYGQNGSFTSDSNGTGNVTASTLSAPRAILLDSTGGVYISCGSRV
                     LYYTSGSTTASRVYGQLGVFTTQTANTGGVTANSLGGPTGLALDSSNGLYISDSGNDR
                     VLYYPSGSTTATRVYGTLGSFTSSIDYRFTISADSLYNPTGLLVDSSNGLYIADSGNN
                     RILYFPSGSIVPSKVYGQSDFTSRTSIYPAATTSSVNGANYLVIDKSGGLYVSGSSRV
                     IYFPSGSTIATKVYGQGGSFTTTTVNNGGISANSLSGAAGLALDASERLYVVDTSNNR
                     VLYY"
     gene            complement(48233..49102)
                     /locus_tag="PLF66_08370"
     CDS             complement(48233..49102)
                     /locus_tag="PLF66_08370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64092.1"
                     /translation="MKCKLSNPRTSVLLLVLFIHSNLYATSGRVSCDSYERNTYETNR
                     AKSLENCMARSNSIPIRLNDKHAFLYLKVPSLGNEYLDYDLGTYDRKVKMLPVLIQFG
                     NSLLNPRKDENKFYQVRSRLKEQIISEHLAIKEKNCIFMAMDSEYLFPIAAKEGLLTV
                     ILYFDYTEDEVMYYTRKRNKNLEYKVPKLYFDQFIHRGLQVYVNFNPGKIYQIEIAIN
                     PNSALPRGSYNATSLDTLETMNTVPGFTITLKELPPNTKFAFDGKDDVGKTYEQVKKE
                     FILYDHTEEAKKE"
     gene            complement(49102..50187)
                     /locus_tag="PLF66_08375"
     CDS             complement(49102..50187)
                     /locus_tag="PLF66_08375"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HMZ64093.1"
                     /translation="MNSYGPDIFRYKYTSQQDDQDTGLYYYNARYYDPVLGSFTSADN
                     VTNATSTFGLNHYMYTEGNPVRYGDASGNFLVPLLMAAYAVAVTAIAITAAVTIAAGL
                     AVYTAMAAVANYAMTAGAMAQTATIATAAFAASAVVAAVRFAVFVDALGVVGAGVGFV
                     HGYYATGTLKGALAGAELGFKIGTAITIAAWVVVGAFVIGAYVGGAIEAGIAFLGLHP
                     AIAWILTYTLNFTIGRLFGELAKSLGRNIGGHWVPINTSNNPYQRQIQQKHGYNERGA
                     YRGQAAGDFLSSSSSLAGGLWDATTFGNDCILNGPTYHSFDRMGAEIVGSGSFYFTEK
                     IMGTIFQNTFDSTAWAALRKPQTLGSY"
BASE COUNT        16049 a         9369 c         8215 g        16459 t
ORIGIN      
        1 aaggcaaatc ctatcttaat ttttaaattc cattgtttat agttaatcaa atctttaaaa
       61 ttcacactat actcctaatc tctttgtttt cttcgatttt tagttttaga tttttcaatt
      121 ttttatgata gaattcatct agaataaagt ctgtatctag tagtaaaata ctttctccgt
      181 ctctgtatcc tgtccctaat attccttccc caacgataga ctctgactga atattttcca
      241 aatctgtcac aactccttct acatgagttg cggctattcc aatgtaacca ctcttatgct
      301 tatacaaaat aattggaatg gtttgaattg actttatatc gatctcaata tttaaaattt
      361 catttagcca taatattgga atggcagttt tgttgtataa aaatctatcc aacccaatcg
      421 agtttaaaat ttccttctgc gatactttta cgatatatgg gtttgtttga atagacatcg
      481 caattctaga tttaccaatt gagaacaaaa ggtaactatc gctttgattt gtgatttctt
      541 tgagaggaat agattcgatt agctttgctt gtgcctgcat tccagcaagt ctcgcaagac
      601 cagagacatc tagtatcaag gctatatctc catctccaag aattgtggca cctgagaaaa
      661 aggaaagctc ttcgaaatat tcacccagag gttttacaac tatatcttct ctatctaaaa
      721 cttcttctat tgctattcca aaataaaatt tttctgattt taaagaaact atgtagtgtg
      781 ctttttcatc tgacttttct tctagatgga atatagagcc tatattggca aagggtaaaa
      841 actgatttct taaattataa atctttttat tgtctatgga ttttattttt gtaggattaa
      901 ttcggatcaa ttcgctcaaa taatgttgtg ggattgcaaa ttttcgattg gcagacttta
      961 caataagaca ggtaacgata gaaagagttt gaggaataga aagtataaaa gttgttcctt
     1021 tttctacttc aaattcgata gagacggttc cccccacttt ctttagattc gagcgcacaa
     1081 cgtctaatcc tacccctctt cctgagagat ttgtaactgc atttgatgtg ctaaagccgg
     1141 gtagaaatag atattcaacc aattcttcat tggaagactt gctcactgtt tcataattag
     1201 ccagattttt ttgaattact ttctctttga ttttctcagt attcaatcct tttccatcat
     1261 cgctgatgat gatttctaca tttcccccat ttaaacgagc ggctactcgt aagtttcccc
     1321 atggtgtttt acctttactt tccctctctt ccttagattc tattccatga tcgatcgcat
     1381 ttcgaatgat atgggtaatt ggatctccta gctggtcgat aagcgcttta tctaattcga
     1441 catcatttcc ttcaatggca acttctattt gttttccagt agtttttgaa atatctctta
     1501 ttaatctagg tattttttga aaaaacaaac ttagactttg cagtcttgtt tttaagattc
     1561 cagattccaa ttcagataat aatttttcaa atcttttaga aataatttct aactccggat
     1621 ttccgctctg ttggattttt aaagctagaa cattacgcgc tagaacaatt tctcccgtta
     1681 gatttattaa attgtttagt aggtttagat ttacatttat acttgtattt acatctgaat
     1741 ctacggaagg cgaggctgat tgcttgggag gagttttctc tggttctggt ttattagctt
     1801 cagtctcttt cctaacgtat acattttcca tattctctgg aactacaacc gtagaaacaa
     1861 attctaaaat caaaccttct tctttcaaaa aattttctac tttattatct gataggatga
     1921 ttaaataata tggaacttta ggatgttcat gtgtatcaag gggaagaatt tttttttcga
     1981 caagacctct tctgagaatt ctaacctttc cttgaatgga ttttagcttt tcaataaact
     2041 cagataaaaa attaaaactt tgaaccgata gatttacaac tacaatagaa agatacttct
     2101 tctttgactt tgcaaaggta attacttttt cggaaagttt aatatcctcc atccattctg
     2161 atttttcttc tagattgggt ctctcaatta tttctgcatc tttagtaatt aaacgatctg
     2221 agcctgtcgt tagataagag ttaaacttat cgactaaatc actcacttcc actaaatcag
     2281 agactgcaag agttgtaatc atttctttca atcgatcgat tgcaagtaac atcaaatcaa
     2341 tcaaatctgg atttggtatg agcctatcgt ttctaagtaa gtttaaaaca ttttcaaagg
     2401 catgagagag tgctggaatc ttttctaatt ggaaaatccc tgagtttccc ttgatggtgt
     2461 gaatccctct aaagagttcg ttgattgcat ttttatcaat agatgtttca agaactaata
     2521 cataagattc caatttcgaa agaatagaga gagaagaatt tacaaagctt tcttttaatt
     2581 ctgtataatc tatctgcgcc aagattctac tcccgagtat tttctaatct tgatacgata
     2641 gtagttagat ttgcttcttt aaattttctg acaagactag gctctgcttg aattgagaca
     2701 ggatatgtat gagttttttg cataagaatc aggaaaggaa tcactacgtt taataaattc
     2761 atggaaatat cttctacttc tataaagcgt atgattaatt gctgatcacc attgatttct
     2821 gagtttaatt ctacaaaaag tccgattaaa tcctctggac tatttctcat atcatttgat
     2881 tctatatgta tataaaactt agatttcttt ctaaaaaaat tcattccaaa gtattttgga
     2941 tagaaagcat tttctgtcga atagtttttt aatttggaaa aagaattcta ccttctgtga
     3001 agcgagatct catttccaat ttttcaacgt cgccaatact tccaaattct agaacatctt
     3061 ctctagactc atttatcaat ttttcatctt tttctaaatc gccgactttg aaatcaggaa
     3121 gcccactctg tctgagtcct aaaagttctc ccggaccacg caattttagg tctacttctg
     3181 ccaattcaaa accgtcattg gttgaaacca tcgcctcaat tcgagtgact gcatcttcac
     3241 tgattttatc tggtgtgagt aaaatacaaa aactttcatg ctctccccgt cccactcgac
     3301 cacgcaactg atggagttga gaaattccaa aacgatccga atgctcgatg actagaactg
     3361 tcgcatttgg aacgtcaatt cctacttcga ctactgtcgt tgtaacaaga agttgaattt
     3421 catttttctt aaaagaatgc atgacggatt ctttttcctg tgcattcatc tttccatgta
     3481 aaagtccaat cttataatct ttgaactcaa aaagtcggag tctttcgtag gcagcaatac
     3541 aagattctaa atctactttt tcggactctt caatcaaagg atagataata aaacactgtc
     3601 ttccttggtt taaatatttt ttaatcgaat tatataccgc ttgtcgcttg tcttctgtaa
     3661 aatgctttgt gacaataggt ttacgtcctt tcggttttgt tttaatggta atgagttgca
     3721 agtctccata gagagtcagg caaagagttc tagggattgg agttgcagtc attgcaagta
     3781 aatctggatt ttttcccttt gaacgaatgg tttccctttg ttctactcca aacttatgtt
     3841 gctcatcaat tacaacaaga cccaaatcgc taaagaccac atcttcttgt aaaagactat
     3901 gcgttccaat tacgagtaac gtctctcctt ctttgagtct agctagtttt tctcttctag
     3961 ttttctcttt ttcttttcct acaaataaat ccattctaga aaaaggagta aattgaagta
     4021 atccttgaat tgtatggaag tgttgccttg ctaaaacttc tgtgggagca agcatacaaa
     4081 cttgaatatt attatccata taatacatcg cagtgagaag cgctgttacc gttttccctg
     4141 agccaacatc tccttgtagt aacactgcca taggaacatc ttttccgttt aactctttta
     4201 ttttttctaa acttgctttt tgatcttctg tgagttcaaa aggaagtctt tctaaaattt
     4261 ttttgactcc ttccgatttg cgaagaggcc agagaactcg tttgatttta tttctctcgg
     4321 ttcttttata ttccatgagc aaattaaaat aataaaactc ttcgtaggca aatcttcttt
     4381 ttgcttctac taacgtctca tctgtggatg gaaaatgtat ttcttgaaag gcagtttgtc
     4441 tttttaaaag ttttcttttt tttaccgctt tttcaggaag gacttcttca atcttaaaag
     4501 atttcccttc cattttatct ataatttgtt tgatgagtct tctaaatccc ctggaatcaa
     4561 gtccttcttt tttaagtgtt tccgttgtgg ggtaaagagg aattatccgc cctgcatgcg
     4621 taaagtcccc ttcttcgtct tgatcgcctg aaataatttc ataatcggga tgagcaatct
     4681 gaagtcctcg aaagtattct aattttccac tgacaactag attcatcccg ggtttaaata
     4741 catgactgaa ataattgact ccactaaaaa aaatcagaga gatcctttca ttgtttgcag
     4801 tcttgactcc tacaatcagt ctactttttc taccatgcgc aagataggaa tcaatcacag
     4861 ttgcaatcag tgtaacaatc tctccatttt ttaaaagaat attcgaagaa aagttacgat
     4921 ctaaatatct tctagggaaa taataaacca agtcgaataa agtatgaatt cctatcgact
     4981 ttaggactcc taatttttta ggaccaatcc ctttgagtgt atcaattgaa tcttttaagt
     5041 tcaaacttct gcctttttac ttaccgataa gacgttagcc gatgtagtgc ttcttcttta
     5101 attttgggat ggctcataaa taacaatacc agatgtaaga atatttcgaa taaatgttct
     5161 atgtttaatt ttttcaattt cctctggagt atatatcaat aaatccacac cccaaacagg
     5221 aacagctaac tgaatctctt ttaatattcc atctaaacgt tcaaaaaacg aaagctgtgt
     5281 atcgcaaatg gcaagcaaat ctaagtcgct tcttttacta tttgttccat tgctgaaaga
     5341 accaaaaaga gtaaccttag ttagattgta ttttttaaaa atcggagata gagaactttt
     5401 taactcttca attttgtcat tcgtcttctc tgtcaattca gctttcataa acacaaacat
     5461 aggttgggaa atagctttgt caatcaataa tgaactactt aaggggttgt gtaaaaacct
     5521 taaaaaaatc taaccacgaa gagcacgaag atcgcgaagg tcaaaatttc cgaggaaatt
     5581 tacaattttt cttcgtggcc ttcgtgcatt ccacccatcg cttgacgctc accataagcc
     5641 aagctatgtg gtgaaaatct tgttcttaca cagtccctta taagtagttc aaatgaggtt
     5701 tgctcctatt gtcattgaaa aatggcaatg ggcttgagag aatcgaaaga gatggaacgg
     5761 agggcttgct ggcgcaggct attagataat tagaattcat ttctatgcac aaaatcaaag
     5821 tttgttcaga aaattatcaa ttttgtatag tagcaaaacc ttaaagtatc tttcaaaaca
     5881 tgtagaactg tttagtatag agaatgttta aaaatctttt catttaactt gactcaccaa
     5941 atttaatttt agtaactgaa tcgtgtgagg agaagacaat gcaaaatcag aatgaaaacg
     6001 tagagagagt aacattgaat tctaatttaa aaagaaagag tatttttcta aaaacaaaaa
     6061 taaaccatcg gtgccaatcg gtgaatctcg gtggtaatct tctaactagc cgctaaacct
     6121 ttttctataa acggaatcaa ctctggcgca tttagaattt gaaaatgatt tcttcccaga
     6181 agctttaaag ttctagattc aggatttgat tttttttgaa atactttttc agttaaagcc
     6241 tggatactcc atttttcaat gatcccatct ccaaaaaaag tctgaaataa attatcattc
     6301 tctcctgcaa agctatatat ttcataggca tctatgtcgt ctaactctcc aaaataatgc
     6361 tctgttctat agcgaaagag ttgatttcct agtttccagt cttcctctcg gatgattcca
     6421 tgcgatagat ctttaattgc atcacttctt aaatttccaa tgaatcctgc tagttttaaa
     6481 aaccaaaaag gagaagcttg cattgcaatc cccgcccaaa atccaatttt ttctatataa
     6541 gatccaccat tgggagaact aatgataaag acttttttta tatttgggag ataactcgca
     6601 ttcgcctctt ttgcataata aagagtgctt cttagaacaa gccctccctg actataggta
     6661 ataacatgaa gtggtaaatt tggttctatc gcgaataatt tttttagaag ttcgtgtaag
     6721 tcttttccat ttacggaaat atgtgctcct tgattgtaac gaatgtagat tggaaaatag
     6781 cctttattta gaaaataatc tccaagccct gtgtcctcgt ctgccatttt catccacaaa
     6841 tgctcatcac aaaaaagtcc tggaacaaat agaatcgctt ctttaaatcc ggattttcta
     6901 tacaaatcca ctatcgtctc agcatttaca tcttccccat tgtaacgaaa caaaagtctt
     6961 attttagaaa aactaattaa atcatcatga gagcttccta aaatactgct aattacttta
     7021 ttttcgtaaa gagttcgaat catatagtct tctgctacat caagggaatg ggttgctact
     7081 ccaaatgcat tttctgtgga gtctatcgct atctccatcc ctttgtttgt cacatccgct
     7141 gattgtttta atttgttact tgcttcttgt agatagacag gtaaatcttc aatttgtaaa
     7201 aacttttcat ctgaaagatt ttccaatgta gaggcagtcc aattcatcga cttatctaat
     7261 gcatctttta ctccactcag aatactgttt gtgacattgc ctgttatatt taaaatttgc
     7321 tttccgaaaa tagttaaatc tgtgtttgga tgcttcatgc tattctccat ggataatcgt
     7381 tcccttcttt gttattacat cgtatgtata gatcattgat tctaaatttt gatttttgac
     7441 ttttggatct ggtttttgtt ttataataca aaccaattga ttcttgtggc tattcaaaag
     7501 tagcctctca aaaaaataac cttctggtaa tacttcatct agtttctcat catgaggatg
     7561 gtatagaaag atctgaatta gatctttatt gtttaaaaag ccgtctttat tggtatcaac
     7621 tgtcattgca aagacgagca ttcgattccc aagatctaga ctttctttta ttacttcatc
     7681 atttttctta atttttttaa taaagtcggc tgggaaataa tctaggatat aaactttttt
     7741 attaaatagt tttttagttt tacctgtatt tagatttaca aaggttatat ttcttgcatg
     7801 attcataacc tcttcaggac cgaagccacc cgcttctctc actaagtcag taggataata
     7861 taaataatta gaccaaataa ttttagaatt ttcgcttaac gtatcttttg gtgactctct
     7921 caccaatatt tcacttgagg tctgtggttc ttcatttagt tccacgcctt tttcaaagta
     7981 aaaccaagtc ttggcaagag agtaaattaa aatcacagtg atgactacca tcactgtgat
     8041 tacaaatcct ttaatttttt ccactgagga aactctctac aatagaaata atttctttat
     8101 actctgcatc tgtcatataa ggaaagagtg ggaaatttaa aacagtgcta cttacttgtc
     8161 ttgcattttc tccaccaaat cttttcttta agaactcttt cgcacctggt tgatctgaca
     8221 ttgcacccgg atagatattt ccaaaaccaa ttcctttttc ttttaggatt gcttctagtt
     8281 ttgttctctt tgcattttca aaaagagtta cattgcagta tccattttca acataatcac
     8341 tcggaggagt aatgtcatta acccctagag cttttagatc tttgtgatat cttgcagcaa
     8401 tttcttgtct agatttgagt cttgcctcaa aatgatctaa acttaaattt acaaaagcag
     8461 cttggagtga atccattcta gaattccaac ccacagcacc atgtccataa tgagtcgttc
     8521 ttccgtgatt cgcaagcatt cttactttat cggctaacgc ttggtcattt acaaaaacgg
     8581 ctcctccatc tcctgcagct cctaaaactt tggcaggata aaaagaagta gtagagattg
     8641 tgcatccttt gtaaacaggc tcgcctttgt attttactcc aaagctttgt gctccgtctt
     8701 caattaagac tacacctttt ttcttgcaga gctctctaaa atcagaaagt ctagaagagc
     8761 cccaaccgta taaatgcacg atcattgctg cctttggttt tacttcatcg attgcttttt
     8821 caaacgctgc aaagtccatt tgtaaatcag ttagatttgt atcaactgtg tatggatttg
     8881 ctccaacgtt tacaactgct tcgaaggttg cccagaaagt agaatcaggt aaaagcacgt
     8941 tatcccctag acccactccc actgcacgaa gagctaattg caatgcgtca gttccgtttg
     9001 cacagctaac agcaaatcca gtttgagtat attcagcgag tcttttctcg agagtagcca
     9061 cttcagcgcc tccgatgaac tgagcatttt tactcatttc actcaccttt ctattccacg
     9121 aatctaaaaa tccgtcctca aatctcttta tatcaataaa tggtactccc atattttcct
     9181 ctcaatattt aaaaaaattt accaccgatg gacaccgatc gcttcgctgc accgatatgc
     9241 caccgatttt ccttataata ctatccgctt ccattcaagc ttagggtttc caaaattaat
     9301 taaataacca attttaattc cactagcctt taaataattc ataagttgcg cttgcatttc
     9361 ttttacaatt gaatttgtaa cttttaattc aagaataact ttttcctcta caaaaacatc
     9421 tgcaataaaa tcaccaacgt ttataccttt ataaacaaca gacaatttag cctgtgattt
     9481 ataatttata ttcattcgat ccaactcaat taataatgct cgatcataaa cactttctaa
     9541 aaacccaacg ccaagcgtat ttgaaacttc catacaagct ccaataattc gatccgttaa
     9601 gtccttttcc aaaaccctat cgtatctctt atcggtgtct tcggtgctca tcggtggtaa
     9661 tcttctctga ttcattttca tccgtggtta aaatcttttc tcctttcggg gttaaatcct
     9721 aaattctaaa acaacaactt ctccacttcc ttaaaatgac tcactgggta gaaggtgaca
     9781 ccttttttca catagtctgg aatttctttt aaatgagcat cattgtcctt tggataaatg
     9841 attttagtta cacctactct cttagctgcc acaatctttt ctttgagacc accaatcgct
     9901 aaaacctcac ctgttaacgt tagttcccct gtcataccaa atccatcgcg aactacttta
     9961 tcttttgcga gtgaataaat ggcagctgcc atcgtaattc ccgcactcgg tccatctttt
    10021 ggagttgccc catctggaac atgaatatga attctcttat ccgaaaaaat ctttccatct
    10081 ggatcgatta aatttcttac atagctataa gcaatgtttg tagattcatt catcgtcttg
    10141 ccaagttttc ctgtaagcat gagaccttct tttcctttta agaaaatcgc ttctactaaa
    10201 agtgtagcac cacccacagc agtccatgca agacccagag ccatacctgg cttatctgcc
    10261 ttggtcattc tctcatctac atagagagga actccaagat attcttctaa ttgatctgga
    10321 gtgatagaat gattcacttt tgttttactt acaatcttat aggcaatctt tcttgcgatc
    10381 ttatcagaca ttctctctaa atttcgaagt cctgcttctc tagaatagga attgatcaga
    10441 gattgaatgg caggacttgt caaagtcacc ttttcttttc cgagtccatt tctttttaaa
    10501 acttttttcc aaagatattt tttgaaaatt tcaatctttt cttctgtgat atatccagaa
    10561 aggcgtataa cgtccattcg atcgactaaa actcttggaa ttgtatcaag tgtatttgca
    10621 gtcgcaataa ataaaacttt agacaaatca aatggcaaat ccaaataatg atctctaaaa
    10681 tttacattct gctctggatc taaaacctca agtagtgcag ccgctgggtc tcccgaatat
    10741 cccgttttaa ttttatcaat ttcatcgagt aaaatcactg gatccttttc ttttaaaatc
    10801 cgaagggcac ttaaaatctt tccggggagt gctcctacat aggttcttct atgacccttt
    10861 acctctgctt catctctcac tccacctaga gaaaaacgat agaactttct acctaaagct
    10921 tctgccactg attttgcaat cgatgtcttt cctactccgg gtggaccaac caaacaaaga
    10981 attgtccctg atttattatc cggatttaat ttcttcacag ctagaaattc taaaatccga
    11041 tcttttacat ccgttagtcg atagtgatcc ctgtccaaaa tcttttttgc atattggata
    11101 tcaatatttc ttttgggtgg ggcttcccat ggcagtgaat ctataatctc tagataatta
    11161 cgggtaacgt tgtattcagg agaattataa tcggttactt ggattctttc gatttctctt
    11221 tttacttctg catagatttc ttctgagacc tcagcctttt ttaagcgatc taagagtaaa
    11281 tcgtatttct tttctccttt ttcttcgccc attccaagtt ctccttggat tgcctttaat
    11341 tgttcgcgaa gaaaaaattg cctttgctgt ttgtccattt tgctattgat ctgatcattg
    11401 atctttcgtt gaacagcgat tagctccaac tctttcttta aatagataat tactttttcg
    11461 agtctttgaa aaacatcttc ttcttctaaa ataaattgaa attcctcttt gtccaaattt
    11521 aaaatggatg caacaaagtc agccattcgt cctggctcat gcatattcac aagagaaagc
    11581 ttcatatctt ctgtaaaaag cggattgttt tgtgcgagct ctttggttag aacaagtaga
    11641 ttgcgcatca tggctttaag atcaatctta cttcctgtgt tcactttatc caccggataa
    11701 tcgaccttag ccactagata tggatcttct tttagaatct ttgtgatttt gaagcgatga
    11761 attgtattga ttaatatatg agttccgcca tcgggtagat tcattttttt tagaaccttt
    11821 gcaataaccc ccatggtata aatattattg accggatttt tctcgctctc ttctttgatt
    11881 aagaggctaa gtccgataaa cccgttgttt ttataaactt catccacaga attcgaaaat
    11941 tttccaacag gaacaatcag tggtgtaatg attccaggaa aaataggacg atttttgatt
    12001 ggaataatgt gtaactcgtc aggtagtatt tcctcaattg tgtctggatc ggaagttttc
    12061 ttggttcgta tgtttttttt ggatacggct tttttcatag aaaatgtttt tacgtcaata
    12121 tttttggtat gagagagtaa aacaatgaaa aataaacata aatttctatt aatcctatct
    12181 attctagtca atcttcttct agttcttttc ctgtttcctg caaatcaagt tagagtaaaa
    12241 tttttctttt acaaactctt tgggcaacaa gtccccgata aaatccctct tttttctaag
    12301 ctagttctca tccaagacta caaaccgatt tctgttttgc cctctcccga taccagatcc
    12361 tctaaacttc cttctatgcc aatcatcgaa acccatggtc atattggaaa ttttttccaa
    12421 acaaaaccag atgatgtttc aagagattta actgctcttg gaatcaaaaa atttatcaac
    12481 ttgagtttca caactggtca agaatttgtg caattgaaaa aagattatgc tgatcctaga
    12541 atcattcact ttagcacgtt caattggaaa cgtctttccg aggacaattt tgctgagaaa
    12601 atgctctccg atttaaaaca agacatcgca aatggaagtc gaggaattaa actttggaaa
    12661 aactatggtc tcactttaaa aaagaaaaat ggtgaaagac ttaaaattga tgatcctgaa
    12721 ttagatcctc tctttgaaga atgtgctaaa tctgggctga ttatttccat tcacactgta
    12781 gatccgatcg cttttttttc tccaattgat gagaaaaatg aacgttatga agaattgctc
    12841 cgccatccag aatggtcttt ttcctccagt gagtttccct cgatcgaaac ggttctccaa
    12901 gaaagaaaca atcgttttaa gcgtcatccc aatctaaaat ttgttgccct tcactttgca
    12961 gaacacgcaa atgaattgga aaaagcagaa aaatttctac tagaacatcc aaatgtcatg
    13021 gttgatattg ccgcccgtat tgacgagcta ggaagacaac ccattcacgc aaaagcattc
    13081 ttagaaaaat atgcagaccg cattctattt ggtgtcgacg gacctcccga tcgaggcaag
    13141 ttagaaatct attctcgctt tttagaaacg accgatgaat actttgatta tttcccagaa
    13201 aacaaacccc gtaaaggttt ctggaaaatc tatggactcg gtctctcgcg cgaggctctt
    13261 gaaaaaatct atttcgcaaa cgctgagaaa ttgtataaat aaagggaaaa agattaccac
    13321 cgatgaggac gatgagcacc gataaaagat acgataattc aaaaatatcc atcgtttctg
    13381 tatctttccg ttatcggtgt cctcggtggt aaattcttaa tttgacaaac cccaacttcg
    13441 tgttccttcg tgtccttcgt ggtgatgaaa tctttttact ataagttttt ttaaccacga
    13501 agaacacgaa gaggcacgaa gggtttttcc ttttaacttc taacccaact tcgtgttcct
    13561 tcgtgtcctt cgtggaagaa tatcttttac taccagtttt tttttaacca cgaagagcac
    13621 gaagaggcac gaagggtgga ataagcacga tgggtttttc ctttttactt ctaacccaac
    13681 ttcgtgttcc ttcgtgtcct tcgtggaaga atatctttta ctaccagttt ttttttaacc
    13741 acgaagagca cgaagaggca cgaagggtgg aataagcacg aagggttttt tctttttact
    13801 tctaacccaa cttcgttttc cttcgtggac ttcgtggtag aatatctttt actaccagtt
    13861 ttttttaaac cacgaagagc acatgcgaag gcgctggtcg tgaatacttg gagcgaaggg
    13921 tggaataagc acgaagggtt ttatttataa aacataacgt cttaagccaa attttaattt
    13981 tttcacatta aaatttatta aaagcccttt gttaatttga gaaagtttca tatatgtcat
    14041 gatttgagcc tcatggatag gcttaatttc agaaatactt ttcagctcta caatcagact
    14101 tccctctata agcaaatcaa ttctatatgc agcctctatc cgattacctt tgtaaagaat
    14161 tggtaagtct ttttcacttt caaatataat gttattttcg agtagttcat aaattaaaca
    14221 ttgcttgtat gtgctttcaa gaagtccagg tcccaaattt ttatgcacct ctatcgcgca
    14281 tttaataacc tgaaaagata tattttcaaa ctccattcat cccctccaaa gaatacttca
    14341 ttattagaca atctgcaaat ctattaacaa ttacctaact tcgtgttcct tcgtggactt
    14401 cgtggtgaaa tatcttttac tatcagcttt ttttaaccac gaagtgcacg gaggggtttc
    14461 ttttttacca ctaatctaac ttcgtgttcc ttcgtggact tcgtggtaga atatctttta
    14521 ctaccagttt ttttttaacc acgaagtgca tgaagaggca cgaaggctgg aataggcacg
    14581 atgggttttt cctttttatt tctaacccaa cttcgtgtcc ttcgtggtaa tgaaatcctt
    14641 ttactaccag ttttttttaa ccacgaagag cacgaagagg cacgaagggt ggaataagca
    14701 cgaagggttt tttcttttta cttctaaccc aacttcgtgt tccttcgtgt ccttcgtggt
    14761 aatgaaatcc ttttactata nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14881 aaaccacgaa tagcacaaat aggcacgaag ggtggaataa gcacgatggg tttttccttt
    14941 ttacttccaa cccaacttcg tgttccttcg tgtccttcgt ggtaatgaaa tccttttact
    15001 ataagctttt tttaaaccac gaatagcaca aataggcacg aagggtggaa taagcacgat
    15061 gggttttttc tttttacttc taagccaact tcgtgttcct tcgtgtcctt cgtggtgatg
    15121 aaatcttttt actaccagtt tttttttaac cacggataaa cacggataga cacggatggt
    15181 tttccttttt acttccaacc caacttcgtg ttccttcgtg gtcttcgtgg taatgaaatc
    15241 cttttactat aagttttttt aaccacgaag gacacgaaga ggcacgaagg gtttttcctt
    15301 tttacttcta acccaacttc gtgttccttc gtggtcttcg tggtgatgaa atctttttac
    15361 taccagtttt tttttaacca cgaagagcac atgcgaaggc gctggtcgtg aatacttgga
    15421 gcgaagggtg gaatgttttt tctttttact tctaacccaa cttcgtgttc cttcgtgtcc
    15481 ttcgtggttg aatctctttt actaccagtt ttttttaacc acgaagtgca tgaagagcac
    15541 gaaagggttt tgttttgctt acactaaaga tctattaaac ttaggagtaa agattgatta
    15601 aacttatcct aaatttatcc tactacctat ctttaaggga agtttacccc tatctttacg
    15661 ctatatatag tctttcttta ctgtttaact aacgccttcc atctacgcct atctgtgttc
    15721 atctgtgtgc atccgtggta aaaaaaaaaa tttctatcac ttctaattga actgaaatct
    15781 caaaattaca aaaataaatt ttttttaaaa aaaattttaa aatttttcag taaaggttga
    15841 cacaaagtaa agaaacgtag cagtattgca attcgaggtc gccatcgctt tgggattttc
    15901 cagatcatct tcgctatgct cgaccccaaa aaatcaataa aggggaataa acgaatgatc
    15961 aaaatgatca gtaaattatt agtcgtgctt tctctttgtt tttttgttac ttcatctatt
    16021 ttcgctgatg atcaagactt caaaaacaaa ggaaaatcaa gaatcatagt tgggctcggt
    16081 atccagttcg actcaaatag cctcggagga actatcgtca aagacggatt ggattccgga
    16141 caaatcaaaa cagactctaa tggtaactat gccggtcagc aaaaagccgt tatcgctgaa
    16201 aataaactac aaactttaga aaacctttct ggcggactta tttcttataa atccacaggt
    16261 cctatgactg cgggtagttt aactctcgga tacgaaaaag atgtcggcga caatttcttc
    16321 tggagagctg gaattaactt ttcaacaaaa atttctggtg gtcgtacttc ttctacagct
    16381 tctggctatg attggtatga tgtaacttgg tattataaat ctcttgtaat tccagtttat
    16441 tttggtatta aattaaacgt tggttcaaga tctgcaattt atgttgctcc aggtttacat
    16501 tactaccaag cagaatggca attaaaagga agaaatgatg gaaacggact cgatgcagta
    16561 accggtggtc tcgcaagaag cctaccagtt gcaagtgatg cagcaagacc aggcgttatc
    16621 aatgaagata caaaattcag cggtagcgga tttggtatga gctggttaac tggtgttcaa
    16681 acaaaagtta ccgaaaaagg atatgcattt atcgaagttg aaaaccactt ttcttacaaa
    16741 caaggaaatg gtggtacaaa gtcttctggt ggtatcgcag caattgctcc tcaacctgct
    16801 tatcctgtaa ccgttggtgg aaccatttat agagtcggct ataagcatga gttataataa
    16861 ggagaaatga atcacatgaa aaaaactttt aaattcttat cttccgtttt atttgtttct
    16921 atactagcag ttaccaactg tcactatagt acaggtggaa gccaaactaa tggagcagta
    16981 gttgcaaact atgtaaatag tttagttcgt tctgctgtta ctggaaactg tgcaattagc
    17041 gttaacctag gatctctcta tgcaggagca atcgtgcaag gtgctgctgg tagtgctaca
    17101 aaatttccac aagcaaaata tgaagcagta actggatcta ccatttctgc tcaaaactat
    17161 gcatcttatg cagctgttcc atacaacaaa aagtatgatg cttttttaac gaactcaaca
    17221 gatggaacct cttggacaaa cgcacttcgt ggaactgcga ttgcaaacca aaaagcatca
    17281 atagatttag gagcatacct agggagagca gcgtttttat gtaatgcaaa cgcagcaaca
    17341 actggagcta ctcttgcggc agctttgact gcttatagaa ctgcttctac tacagcagga
    17401 gccgataact tcacggctgc agagaatact gctgtgacca ctctgttgac tgctcttgga
    17461 acaaactttg caacaattga aggtgcattt actggctttg gttgtgcagc tataactggt
    17521 gctctagcag caacatatac aaactttggt ggatccgctc aaactgtttc tctttacaac
    17581 gctagattag cttacacaag tggtcttgct ttaatggcat gtgctagaat tccaagaagt
    17641 agctgttcta catctgctct tgttacaaca actagagatg cagatgttgc tagcttagta
    17701 gctacttacc aaacaatcaa tgctactcct gaatgtagaa agccaagtac tggtctttta
    17761 gcttctgttc tctctaccgg agcaacagga cttccaaaag aaactacttt cttaggagca
    17821 aatacaacga ttgatttagg tggtggtaga atttaccaaa acactgctgc tgttggtggt
    17881 tctgaaggaa gtttaactgg actttcttct actggattac tcgctgctag agcttatcct
    17941 aaatcaggag ctctcttagg gattgcttct tcttttactt ctgctcatcc tatgatcgca
    18001 ggaacaaccg catatcctac agcaactgac ggatctgttg caactcctta ttacggtggt
    18061 tctaacatca atgctacaac tgttacttct tgtgatgctt taggctttgg taactatgga
    18121 ccaactccaa tccaaacaac tgattcttta gcaactgtaa actctaaaaa gactttaaca
    18181 gatgtaaaag agatcgttta tgcattctct gcaaacaata ccgttgcaaa agcatatgaa
    18241 acagcaattg cttctgtttc accaactact gcaaccaacg atgcaattgc atgtaaccgt
    18301 caaatgagaa aaactactac catttcttct gcacttggtg taggaacaac attacctgat
    18361 atcaacgctg gatctggtga tggtggtgca acttctttag tatctgcttg tgtatacggt
    18421 tcaaacgtta caaacagaac agttgcagct gcagttttct cttcgctttt acctggttta
    18481 acatcttgcc cacaagcagc tgtttccggt gcttctagct ttggtgtaac tggtttaaca
    18541 actttctcgg cagcttcttt cccagatggt caatagtctt tagattgatt caatcagtct
    18601 gaaacttgta gatcaagttt gccttgaaaa ccccgccctc aaaagcgggg ttttttttta
    18661 cttgcattgc aatgattctc taaaaaaaag tatttgaaag attctataaa atttgcattc
    18721 tggagacatg cctttagtga gtgagtttta tggaataaaa atttatatgt attgggatga
    18781 tcatgcacca ccccattttc atgcagaata tgatgaatac aaaatattag ttagtattaa
    18841 agattcgact gttatcaaag gagcatttcc atcaaaacaa ttgaaacttg ttagcttggt
    18901 gtgaactaca ctctggtgat ttaatgaaaa actggcaaat ggcagcagag cacagagaat
    18961 taaaaagaat cgacccttta caataggaaa tcaatttcta tgatacatga aatagtccaa
    19021 gtaattccaa aaccaaattt taaagtatat ttgtatttct cggatggaaa aattaaactt
    19081 tttaatgcag cagaaattat taaaaaaggt atattcaagc aattgaatga tttagatatt
    19141 ttcattggta aatgcacagt gctgaatcac acactcgctt gggatataaa aggaaattac
    19201 gatccctaca actgcctcga tttagatccc gaagtcttgt atcaaaattc agatgatgta
    19261 aaagatccac tcacgaaata actcttttta tacttgcatt ttaaaaatga aaactcgata
    19321 gtaactctat gactctgctt gcagaaaaaa aaatcttaga atatgaagac attgactctc
    19381 tacccgatgg attttatgaa atcattgacg gagaaagaaa agatatgtcc ccctcaggat
    19441 ttttacatgg attaatcgaa ggtagccttt ctgaaatttt aaaagagaat ttgtctaaaa
    19501 caggatctgt tgcaacaggg gaaataggaa ttctaattca aaaaaatcca ctacgtgtga
    19561 gaggtgctga tatagtttat atcacaaaag aatcgtatcc aaaaccacca attggaattt
    19621 tagaaaaagc acctgagctt gctgtagaaa tattatctcc tgcaaacaca acgacagaga
    19681 tgaatgggaa gctaaaagat tatcttgcca ttggtatacc gcatattgta tatgttgatc
    19741 cagaaaatga aatgacaact gtctatgaaa acaattcctt tcaagttttt agctttgagg
    19801 aaaaatttag actctttgat tttgaaatca aaatgagtga tttactagtt taacttatgg
    19861 ataaaaaaac aattttttta ctgccactgg atttgaaagg aatcaaacag acgcgaatca
    19921 cgccaaagtt tatacgcgag atttatgatc cagacaaagc aaataaatta ccagaacaca
    19981 ttgagaagaa aaagaaaaat atttttacag cagaattgaa tcgactgatc ggaaagattt
    20041 ccgaaaaaaa caaagcagag aaattgcaag aattagaaac aaagatcgat ccgaacccaa
    20101 cgttagaaga aatggaagaa gcagttgcgt tgatcgagaa cgaagaaaaa gaaaaagagg
    20161 aagttgaggt tttgccacaa gaaatggaaa ccctacccaa atcagaatca ctcgacttac
    20221 tcaaccaaat ctatttaggg caaaatgcaa aagaaaatgc actaaaatac ttagaggcaa
    20281 tgtttcaaac acttggaata aaagcatatt gtgttttctt ctatgaacca cattcttata
    20341 cctactttcc tgttttttca aatggtctca cagaaaaagc acagaccaac ctactctttt
    20401 atgcaaacga tagatatatc caaaatagaa cagaaggata cactcatttg ttttttcagc
    20461 cagtgctagt gaatgatata tttttcaaaa agaaactgtc ccctattgag tttaatcaat
    20521 tctccagtat cattctaaaa ttcttagata aacaaaatct aaatggtctg attgctctct
    20581 ttgtagataa atacaaagca atctcagaag aagagataga cacactttgt aattcgattg
    20641 ataaaaatat tgaaccctta gttccatttt tgaattccta ttttgaatca gaaaaaaaag
    20701 aaagactgga ttcttttgat ttcatgagac aggtgattca tggaattcaa atccaatcaa
    20761 aaaatttagg agatgagttc tattttacaa aattaaaaat cacgaacttt ttatcggtag
    20821 aggacgcagt aaacaaaaag aaacaattga ttcacctact aaaaaaacat cttctcgaaa
    20881 aagaggggat aatcgatttt agccacaatg aaattttatt gctaacaaaa gaaaatgaag
    20941 gcggaaaaat cattcaatac atttctgaaa atgcacaaac tgattttcaa tttgagatct
    21001 ttccacttag atacccagac aatggtaaaa atttatatct ttatttttag tctttctctt
    21061 gtagctagca ttcaatcaga aagcatatct agaaaagaac tctccatact tcgagaaaat
    21121 tgtttaaaaa cctacaaaga aaaaaaatat tcagaagcaa ttccactcct agaaaagtat
    21181 ctttctttta gagaagaaat taaatttaaa actctacttg ccaaatcgat tttatttaga
    21241 acagatctaa tcgcacccaa agaagaggat gaaatattta tccgagacga aaaaattcac
    21301 actgcaatcg aaaattacaa aaagtccgct agagtttttt cagaggtcat tccctattta
    21361 gaaaaagtca caccaaatga caagtcgata ggcgaattgt attttctctg ggctctgtca
    21421 gaacaattta gtaacgaaag agaaaaagca attccactct acaaaaaatc agctctccta
    21481 aaaaaagatt tagccgccct agctttttat aatataggtt ctatctatga atttctaggt
    21541 caaaaaaaag acagtgaaat ctatttttca aaatacaacc aagcatccgt ggataagaaa
    21601 gaggaaattc gtgaagaaag cagataagaa atttcaaatg ttgtttactg aagatgaact
    21661 ctccaattta aaagaggaag cagcaaaacg aaaattaaca atgtccgaat acattcgcat
    21721 gtgtatcaaa aatgatctca caaaaaagac ctcctactct aaaatcatgg cactacatac
    21781 tcttaaaaat ttgaattcca caacatgaaa aaaattttaa taacagaaaa ggtcttagaa
    21841 gaacttctct atggatctaa aaaaaataaa actctcattg cgaatcaatt tgaagaccat
    21901 ttaaaaaaaa atgattcctt tttcgtatcg attcttactg tagaagaaat tctaaaaaaa
    21961 gaagagtcga ttgagaacag aaaaattatt tttaaaaata taaatgaact ctgcgatgag
    22021 attattgaaa taaaattaga tgatagttct tctgcacttc gaatagaaac agaatactca
    22081 accgatcata gaaccgcaat ggagctaaca gttgctagcc gcaaaggaat cgattgtatc
    22141 tatgacacaa ccgatcgatt tgattttcaa aaaatgatct cagtgattcg gctgattctt
    22201 aaaggtgaat gaaactggat tagatggaat acttttttgg tctcttccgg ttgtatcatt
    22261 gtaattttta ttgcaagctg ttaacttaaa atagtaagta agaccgggtt taaaaaagag
    22321 taaattttta tcttttcgat tcaatgaatt taatgtaatc aaatcgttgt ctacacattg
    22381 ctttaaagtt ttataggagg aaaccaattc ttttccgtcc tccaaaccag tgatgctttc
    22441 ggatttttca ttgatgagaa ttgttccaac cattcgatct ggactcactc cataatggat
    22501 caaatacttt ccgcctgttc taacattttc ttcgtgattg gaaatccaag ataagcacac
    22561 accaagagaa ttttccattg cggctacttt tagacccaca ggagaactcg gaggaagaga
    22621 ttctgcatat ttaatttgca gtttattgag tgcgggtgaa tgctttcctg aataatcaga
    22681 gcgcatgatt aatttccact gtatgtattt aaaatcaatt tccatcgcat ctttaaaaga
    22741 ggaagaatcc atccatttta tatctgtatc atcttctgta aaaaaagtat tggaaacacg
    22801 aaaatgaatt tctagatgag tgcctgctgg tttattcgaa tcaaagtcca aggataaaag
    22861 tctggaaaaa gaattttttg tttgtaatac aggagaatag gcaacacctt ttgcttggct
    22921 tgcaaattta atcatatcat catattgaac accacgatat ggttctgtta ggcgctctat
    22981 atcaggactt cccaaaccaa ttaaaaactg atctaatttt ccaaagaatc cttttccaat
    23041 gaggagcgga gtagtatcat ttggatgaaa cccaaacgga agagaagttg gattttctgg
    23101 gctcatgatg gcttcgattt cagattttgc ttttccatct tcatacaaaa ttgcttttcc
    23161 gctcattggg tctatggcta ttagaacatg ggtccactct tctgctttga gtttatctgg
    23221 gctttttaaa aataaggatt tggattctgt tttggaataa gagaacatat tgtgaaactg
    23281 aacttcgatt ttgttattta caatgtcaca ttcaatccca aacttctttc ctccggtgat
    23341 taaattttta gtaaacacga cagagtattg ctctgattca cccggcatta ctaaaaatga
    23401 aaaatagaaa ggttctgtaa aatgtgtaga atttagaagt cgtccattgt ttgttcctac
    23461 agagatgtag ctattgctag ctgcaaaaga agcataacgt cgccctacta acgttttatt
    23521 ttcaaccaca tcataggaag aaaaattaac tgtataattt cctgttgcat cttttaactc
    23581 tgaggctttt ttattctcaa aattaaaaag aagctctgca tttgcatctg acttttttgg
    23641 acttaactcg agtttgaaat tctttgcaga tgcttttaca attcgaatcc ccttcttttt
    23701 gagttttgaa aagtcttctt tgtcatcaaa aataaactct cctgcaaaaa gagaataact
    23761 aaaaataaat aagaatattc gaaaggtaat atttaagaat tttgaattca ttctaagtcc
    23821 aataggttaa tacgattttc gtgacgtcct gcttcaaact tggattctag ccacttcttt
    23881 actattcgga cagaaaggtc ttttcctaaa actcttcctc ctagaacaat cacgtttgca
    23941 ttattgtgtc gtttggacat ttctgctgta aattcatcat gacataaagc agctctaatt
    24001 cctttgattc gattggctgc aatagacgct ccgatcccag ttccacataa agcgataagt
    24061 ttttcgaact ctcccttttt gagacggcta caagcctccc gaataacgac aggatagtct
    24121 acggacgctt cagagtgaac cccgcaatct atgatctcaa ggtcagaatc aaactgtttt
    24181 cgtaaaaatt cttttagctc aaaaccaccg tgatcggata caattccaat tttttctttc
    24241 aaactttcct cctaaatcac ctcaaaggtg taacaaataa tgatctaaaa tctaaaaaag
    24301 ggattttaat tcatacgctc ggcagcagct ttttttagtt catccactat tttataaaca
    24361 tcaatttcat atttctcaaa aagataattt tttgctaaat catagcgaat taaattaata
    24421 ttaaaattga ccttagcttg aaattgagcg agttccgctt gtgtgtatgc atcgagtgca
    24481 ttttttacag ctaacgctga atatcttccc tgtaaaaatt tagaaacaag tccgtcataa
    24541 aatttcttag tttcttcttc tgtttgcttt gcagtgagta aaacagaata ggaagattta
    24601 attgcatcat atcttgttct caattcatct tctacttctt ttcgaaatgc ttcttcttga
    24661 tctgtaatct gttttacact tacttgtgca tctcgtatcg aagttttcac ccctttgtcc
    24721 caaagtggat aagacattgt aagttcagct ctatactcag ggtatttaaa ataacgaatt
    24781 ccattttgat tcatgtaatt gaaattttct tgtggagagt aaagagactg cgctctagaa
    24841 cttgcagcag cacttatttt tagagagggc atatcttcat cttccgcaac actcaaaccc
    24901 aatcgagcta gatctttttg cctttttagg ttttttagat cgattcggtt ttcataggca
    24961 tattcaatgt ctttttcaag atcgataggc ggcatggtgt ccactaaatc agttactccg
    25021 ctgatttcaa cactaggatc aacacctagt attctttgaa gtcttctttt agcatcgttt
    25081 ctttctagtt tagaacgttc tacttgagac tctgcacttg ctacaatcgc attccactga
    25141 ctttcttcaa atcgttctgc aattcctatc gcttgttttc gagcagtgag atttcgaatg
    25201 ttttttgagt ttgcaagtaa tttttcaaaa gttgttacag cagaatctgc aacagataaa
    25261 gaccagtatt caactagtgt ttgtgtcact aattgcgtta gttgaaatat caaagaatcc
    25321 tgttgaatcg cagcttgggt tcttagagcc tgctcttttg ttttttctgt tcttccaaat
    25381 gaatatttta atagttcctg gctcaattta aaagtaattg ctccagtata aagtggcttg
    25441 actcctaaaa atgcgaagga agaaggagtt gtgattggat tttcgagtgc atttgtatca
    25501 tatcgaagtg tgctgatttc tgttgaaaaa taagttcctg ttttaaattg cttttcaatc
    25561 ccagcgctca gtcgatctgc ctgccctttg gttccaccaa agaagttatt taagttaaaa
    25621 ggaagaacaa actcttgctt gctcgcttca gcaattaata cccaattgaa tttggattca
    25681 tttttataaa ttggagtgtc tgcctttaaa atttcaaact tagcattctg cacagaagca
    25741 tttgtatcaa tcactctctt gactgcatcc ttcaaagaaa gacgcatcat ttttggattt
    25801 gactcctgcg attgtatttg tgtggtgagg aggataagaa caaaaataaa aagggctctt
    25861 attgttttat tattagaatc tatcatattc agttgaactt ttgtgagtat aataactgat
    25921 tagctagcgt atatgttttt gtaaatcttt ttcaaaccta tagaaaaagg gataagggtg
    25981 attttctatt tttgtgattc cacctctctc cagattttgt tgcaatcctc tttccattcg
    26041 gaaggacaaa ccagaaaaat accgagtcct ttattcttgt gaaatctgta tttttcaaag
    26101 cccacaaaag atttaatttc atgattttct gttaaataaa aggatgtttc atatccagat
    26161 tgaaattctt tccgaataaa attagttcct tttttaaccg attcgctcag tcctctccta
    26221 gcatcccaaa caaatggata catttctttg ttctgtgaat ggatatggta ggtttgtcct
    26281 atactcaata caatcttggc tggtgattcg taaaaaattt gattttcata acgaaagtat
    26341 tctgtttcga aactctcctc tttttttgct tgtggcaaaa atattctaaa ataagaaact
    26401 ttccaattgt attctttttc ttttcgggca aatcgataaa gatcggtggg gaaaacatat
    26461 ttaaaccgct catccaattc ataaaagaaa ccaaagtctt tgctttcaat aaatagcttt
    26521 cgaattcctt tttgattttt cgtataacat ccatatggct ctgttaatat ttctaattct
    26581 tctcttttgt cttgttcttg ggagagaagc ttattcaagg ttttttgggc ttcgggatta
    26641 aacacttttt tagattggtt gcgcattcgc tcacaaagag ctaaaccctt tagaagcctg
    26701 attgcaggaa agacttttct tttttcgata agtgcctttg cctctcccat atgaatctct
    26761 agttgggaag aatccggcat tgcctctttg taggaatcat ccactgagaa ttcaatcgct
    26821 ccatcccatt ttaaaaaata gcttctacca aataaatcag tttgaagctt tggcgggggg
    26881 tgaatcttgt tagccacatc ttgtgaggca agttgcaaag taagaaaaaa agcactcagt
    26941 aaaaatataa attttttctt tgaactaaat ttattttcca attgtaaatt aattactctg
    27001 ccaatgcttc tgtaacaatc tcatagagca tttgtctatg ttcttctgct ttgggtataa
    27061 cgcttaaatg tcttgaaagt gaaattaaaa acttatcatc taaaactttt ccattgagcc
    27121 attttccagt catatccatt agaatatcaa tcactgactg aaattccata accccaagac
    27181 caactagatt tgtagcaaac ccaagggctc cttctgtcgg gatatacatc ttgaaacttt
    27241 tgattttttt tagatcaaca ccttttggta aagcttttac aacattttct tccatatcga
    27301 tcgccatagg accaatgttt gtcatattga tcagcacagc atgaacagaa tttaccttac
    27361 catgaattgc atgacattca tctccaagct ttcggatctt gatcgcatta tcccgagtaa
    27421 ccgcatctct tccgaggaaa ttgatatgat ataaataatc ttctagttct ttacccgaaa
    27481 tgacaccgag taagattaat tgatacaaag taaaaatcaa ataatcactt tctgtattat
    27541 cacctaatag aatttctttt gccttcgttg gttgataaag tctatcctgt aataaaatcg
    27601 ttagtttgta tgtaagctga tcaaaaagac tttggtaagt agaaccaaaa aacttttttg
    27661 cgcgatcaac tgcaggtcca actccttctt ttaatagatc attcaaattg agtacagaat
    27721 tgacaacttt gtctacaaca cctttaattg ttccatctaa atacttgaga tggagtgatt
    27781 ccatttctat cccatgattt cgaattgttg catgaagagc tcttctaaaa tagtgaggac
    27841 tcgcggaaat aaatgcaagt ggagaacctt ttgtatcctc tcttagtttt ttataaaata
    27901 atggcatagc aggaagagga attttttgat caggtgtttc aaataaagta gaaaacattc
    27961 ctttttttga gcctaactcg gttgctagat aggtttgatc aatatccgag gttgtgatga
    28021 aagaattata cttttcagaa agtattctga gcttacccat accgactatc gttacttttg
    28081 taatcttact atgctctttt aaatttaaat aagcaatgtc tttcgtatat tgtctatagg
    28141 aatcgcttcc tttgaaaatt acatgaaatg tataattacc cgcttttagt ttatgcgtta
    28201 gttcataaga aaaaaatcca tcctctgttc caacaaagtc aggagtctca taaacctttt
    28261 ttcctttttc tgaatagatt tccaaatgca aaacaggctt tcttacaggc gcaagactaa
    28321 aatctaaaaa gggagttatt tcattttctt gtccttgaaa aagtccagtc atcaaattcc
    28381 ataaatcatc tgcttccatg atatcggtaa ttccgacatc aacgacctgt cctcgcacaa
    28441 gtgccctgcc ttctcttcct agtgaactac tacagatagc aattcttttt ttgtctttag
    28501 cgcgaacttc tctttcggag ttctcttctt gtgattctat ctcttccaac gcaatgactc
    28561 cctttcaatt tcaaagtaaa aatctgtatt taaaaagtaa accatttcgt aattctttct
    28621 agaatatgat atttgggaca gagccttcgt gcaatgctgt caagttaagg tgattttcaa
    28681 agattatccc gataaaagat acgataatcc aaaattatca atcgtttcta tatcgctctg
    28741 tgatcggtgt cctcggtggt aattcttaag ttgtcagcac agtcctttgc cacagcttta
    28801 ctgccgatca caaaacgatg aaagacgatg ttttctttca accgtttgcg attcgcataa
    28861 atggatgatc ttctatcata tttagataat cttctccaaa tagatctatc gtgtttgcag
    28921 gtgctttgtg gtattgcaga atgagaggag ccaattcttt ttctctatct gtgacagcaa
    28981 tttttctcat ttgctttgca agtatttcag caagacgaga tactcgatcg atatcggaca
    29041 caacttccaa ttcttctttg ttaaagcctt ggtgtgcaat cacttttaca aaatgactgg
    29101 gcattttcca aatttcaaag gctctagcgc caatctcgat tgaatcaatt cctatttcat
    29161 ctctctctaa atcatacaga gatttaactc caaactcata aagtcttaat acttttaaat
    29221 acttttcaac aaaatttaaa gcgagtatat tcattccgat ctttcgaaga agagaaacta
    29281 caaagatgtc agcggctaac gtctttaaat tgagaggagt taataaatca taagaaataa
    29341 gtgaggttaa gactggtaat tctcttaggt attttacaaa aacaggatcc ttcaatggat
    29401 tcgtaagagt cttatcaaat tcattgatca ccattctacg agtgattttt gttccaatga
    29461 gagtaattgc atcttttaga tttcgaatgc ttcctgatct accgtaataa gctgaattgg
    29521 cagatcgaat gattgtgctt gtcatcgcaa tgtctgggct gattattttt tctagttcgg
    29581 ctacgccgtt ttttggatca tctacatcaa agtttacaac actcgcaaat acaaggggta
    29641 gattgggaag tcgtatttta cttaggtcga tcttttcaat tttttcatcg aaggaaccta
    29701 aaatatctct tcgccctagt atcactgaaa acttacgaat gattgtgtct gtttcaaatg
    29761 gttttacaat ataaccatga actccaactt gcaaaacttg ttttacaaat gccccttcat
    29821 tatggcttgt gatcaacatg attttcattt ttggatagag atttttaact tctttaattg
    29881 tctccattcc attcataaca ggcatttcct gatctacaca gataatatca ggcttgatat
    29941 gcatttcttt gagttgattg agtgcgtgaa gtccatcccc ggcttctcca atcacactaa
    30001 agctaagctt tgctaaagtt ctttttaatg cggtgcgaaa taaaacactg tcttctacga
    30061 taaaaacatt gtaaggttct cttgtagtta agtttacacc taacggcagt tttgctaaat
    30121 tagagccaag cacggatata ctattctaga tcactccgtt cttggcaact atttttaagt
    30181 gattaaggct ttaaaacaaa aaccttaagt tcgcgtctat ctttcttgcc cgcagtccaa
    30241 acaacatttc catttatata tactgggtca tctttgcgat ttaaccgtgc acttcctagg
    30301 tcgactggtt cttgcaaaat ttttcctgag gaattaaaaa taatgtattc tgttcttata
    30361 taggaattgg ctgaccattt ttcataaact gctaaaaatc gatcctcttc taattggatc
    30421 aatttaggtc ttaaaacatt ttcagattct ttctttttga aattggtgag ccactgaaca
    30481 cccactctct tttgaggata tttatttcct gcataatcaa caaatgaaat ctctggggag
    30541 ttttctccat ccgaaataac aatatcttta ctaattaaat attgagattt tgaatctact
    30601 tttacttcat taaaattttt cgcaactttt agcaaaaata aatttctaga atcattcaga
    30661 taaatttcag cattggaggg attaaaatcc ttttcagaag cacctaacac taagtaacca
    30721 tcggaaaccg aaacaagtcc gcctaattct gtaaatgtct tctggtacat tttacctgct
    30781 ttataggtaa aaataagtct tcctgttttc tttttagtaa tttgaattcc gcgaggaaat
    30841 gcatcagcca aatctaaatt cacaaagtct tttccatcaa aaatcattct ttggtcaaag
    30901 gaatgagagg cagtccatgt ctggctttct ccacttaaaa actccattgt ctccgagttt
    30961 acgacaaaca aaataccact ttgatgattt agtccgtctc ttttgttttt atgttgggtc
    31021 tttcccagat ggacggcaac ttttccattt ccatatacaa ccctagacgt tcctgcatca
    31081 attggtttca tcacatcaaa gtcttctcta ttgatcaaag gactaaatga tttaacttcc
    31141 tttcctctcc cgtctaactt tactaatttg atatttggtg aaaagattcc atctagattg
    31201 ttttttgcca caactaaaaa ataatttcct ttttcgtctt ttgtgaatcc gccaaaaatg
    31261 ggaaaattgg tattgtaaac aatttcatcc aatggctcaa aactctcatt aaactcttga
    31321 atatgaacct ttgttccatt ggattccatc caagctacag tgagtttttt accatctgta
    31381 taatttacta tatgaggggg agttcccgtc atttcaaaat gggaatcagg agaaatattc
    31441 tgttcttcaa accaactatc aaatggttcc cctgttacag agattgtgta gattggaaga
    31501 ttttctggtg tagaatgttt ttcgatactt aaaatttcag agtccggtat aagagcgatg
    31561 atacctttat caacggttac acctgcccca ccctcctttg taaatcgaag tgcagaatct
    31621 aaatgaaatt taccacctac taaatctttc gatttaaaac ttttcattaa tttaaattta
    31681 tgatcatcaa ttacataaag agaataatcg ccattaaccg caggcttata taatactttt
    31741 agaaactcat cataattatt tatattttcc tttaaattct ctccaaaatg agccataatt
    31801 cggaaagctg agtattcttt ttgcgaaaat ccagttatag tataacgttg aacttcaatc
    31861 gatttggctc tgtaggtatc atcatatttt acttgaacag aaatttctga agtaatatct
    31921 tcagtaatat ctttgtatcc aatgtattct tttaaaattt taggtccaga aacctcaatc
    31981 atcaaggaag aagatggtgc tgtatttttt actaaataag gtttctttct gtttgccgct
    32041 tgcgaaaaca aagcagcgga acataaaaag aaaatcaaca ctagaattat ttttgaaata
    32101 ttttttttag gaaatttatt gataatattt ttcattttaa cctcgaaggc gttagattat
    32161 agaaattatt tttttactca agtcattttc tgaaaaagat ttaaaagaat aaaaatttga
    32221 gatgacaaag aactatttct agtaaaaact gatgaaacta tcctagtaaa aaaacttgaa
    32281 agaaaatgaa ttatgaatgt aagagattta tataatatct acgagtctat caaccgtgac
    32341 attgcgaaac tgaaaacaac tcgcagtagc atacctgttg acagggaata cactacaatt
    32401 ctacaaagta caattacttc agaaattgag aaatttgaga gtttaaaaaa attgattcaa
    32461 gagttggaag tagaaattcc tgatgatttc aaaaccattg aaaaacaaag tgtagattat
    32521 tcagagacaa ctttcttccc accgatgctc taatacaaaa tcattccaat gaaaaagcaa
    32581 ctgcctgata atctcaaaaa gaaactaaaa aatgaagaag ataaaaattg gagtgatcaa
    32641 caaatcaatt ttattttaga aactcacaat gcaaaaattg tagaaatcac aggttataaa
    32701 ccacatgcag attatataaa taaaacctgg gaatttggca aagcgagacc tgaatacaga
    32761 gagatatgtg aatcttgcca atttccctat gccttttatg cagagccaag aaattggtcg
    32821 atgggttctg actcagatgt ttggaatctt atgaaatgcg caaatgtaga ttgtggtaaa
    32881 tatttcgaat cctgcctttg atactttcct aattcaaatt taaaaagata aaaaacttta
    32941 ctgtgttttc ttttgaatcg aacaatgaag ttattttctg attattaaat tcaataacat
    33001 actatttcat cgaaactaga aaacacagaa aaattcaaga gtataaaaat ttaaaaaaca
    33061 aaaataagag acaaagaaat tttctatcat acaataaatc gtttctctca aatgctaaaa
    33121 tagaattgtc tattaaaaag gtttggttga tttacatact tctgagtgtt ctcgctctta
    33181 cagattctcg atttactaaa attgattcca atcaaatctt tacaaaaaaa tactttcgcc
    33241 tcaatctcat ttcgcagata acactacata attcaatttc gaaaataaat attcttcaaa
    33301 taatttagaa cgaatgaata acatgaaaaa tatttttcaa aaaaaaacat gaatactaaa
    33361 atcaaaacat aagattctat tttccacttc gtaaataaaa tgatttttat ccatatcaat
    33421 ttcaaagcat tctcattttt tctaatcaat tcaaatcaat gattgaggtt atgtaaattt
    33481 aaaattttta agagaaaagg atgttcttaa gaatattaga gaatcctaat gaaataataa
    33541 aatcaatagt ctggggtagc tcaactgcaa gaaaaaaaat tttacaagcc ccactcgctg
    33601 actgcaatcc tattctgatt tttcaatgat gatagaaaag tttccccaaa acaaaatcat
    33661 ttcttttatt atgaaatgat aatctaaact gagtcaataa aataaagggt ataatcatat
    33721 actctactat ctagactatt ttatataaag aaaacttatg acattttcaa agctcaaata
    33781 aattgcggct aatcataatt aaccacaaaa ataaaaataa gaaatatttt tattctgtca
    33841 cataacgacc aatgagacat tattccaatt tagtctcatt gtacttttta ttttgaattc
    33901 ttattaaaaa ctaaatccta aatcttgtac cgtcgaagat aaaattaccg atgaaaaaag
    33961 aatcttacaa aaaaaagaaa cttcctaaca aaaacgataa agttgtaaat ctcaacttgt
    34021 ttagaatcaa aaaatcactc acagaagaag gattcgaagt gattgaaaat gaaaaagggg
    34081 aaatcaaact gatcctaaga cttgcaaaat aatgttttac aaagagagtg aaaatctctc
    34141 tttctctcag cttgcacccc ttcttcaatt ttcaaaaaac aaacctttct atcaaatgaa
    34201 ttctatttct cttttggaac tgtatatgac tttggaatgt gccaattaga attttccact
    34261 tgccatatcc tgcccattta aaactctaat cctagttgca ttgcccgctt agctcagttg
    34321 gtagagcgtt tccctcgtaa cgaaaaggtc gccagttcga ttctggcagt gggctttaca
    34381 ggaaggaaaa tgccgtataa agaaatgatt ggaagtttaa atggatctgg acagaaacat
    34441 gcgattgtag tttccagatt caatgagttt attacagaaa gtttactaaa aggtgctcta
    34501 gactccttga aaagacatgg agttacagaa gaggacataa cagttataag agttccgggt
    34561 gcttacgaaa tcccctttac tgcaaacgct ttagcaaaaa caaaaaagca tgatgcagtg
    34621 atttgtattg gggcagtgat tcgcgggtct acctctcatt atgactttgt agcgggcgaa
    34681 tcagcaaaag tgggaaatat cgccattgaa aattcggttc ctgtgatctt tggtattatc
    34741 acaacggaaa acattgaaca agccattgaa agagcaggta caaaagcggg aaataagggc
    34801 tttgaagctg ccacaactgc aatcgaaatg gtcaacctac taaagcaaat ctaatggcat
    34861 ctagacataa aagcagatca atcgcattac aggcattgta tcaaattgaa cttacaggtg
    34921 tatcgatcgg ggatgcactc agtttcagtt ggtatgataa ggttttggaa gaagaagaaa
    34981 aagaacttgc gacacaatta attaaaggtg ttgtgaaaaa ttgggattta ctcgatacaa
    35041 taataaagtc ctattcaaaa aactgggatt tcgaacggat ttcggtggtt aatagatgta
    35101 tattgcgatt gtctatctat agcttgatca atcaaagaga cattccaccg aaggtagtaa
    35161 tcaatgaagc cattgaacta acgagagaat ttgaatcgga gaccgccgtc tccttcataa
    35221 acggaatttt agatgcagta tataaggatg aattagccaa actaagcgga gattagctat
    35281 ggaaccaatt aaaaaaaata gactcaaatt cgaatctgta gaaccagaaa caaatcctgg
    35341 tatcacacaa tacgaaacag atgaatattc cacaatgcaa ctacgacagc gcacttctac
    35401 tccgctccct tggattctac tttttttagt tctaatcatt gcactcggtg gagcttcttg
    35461 gtattacttc tttggtcgaa atggcgctga aggcttctct aaaggagatc tagcagatga
    35521 taaaacagga agtctagacc gaatcttaga aagaccttat atgccagcaa gttcaacaaa
    35581 tcctagactt gcagaatgta ttaatttgta tctagaaaag tatcaaaaaa aagcagaaac
    35641 aaagtgtgaa gaatttttaa atcgccctga atcagaccaa gacaagtcta tcgcattaac
    35701 agttctagga gttattcatg atgaaaaagg acgttatgca caagctattg agtatctaag
    35761 aaaagcaatt gcttatgatt ccaaaaacat ctacgcttac tatgatctag caatcgccta
    35821 taaacatgat ggtagaatgt ctgatgctag aaccacaatt caaaaagcaa aggatttggc
    35881 tccaagagat gagaaggtat ctttgcttgc aggcaatcta ctctatgatt cgagtgatcc
    35941 aaaagcagca attgaagcct ataaagatgg actctctagt tcgcctgacg acccttctct
    36001 tgtttataat ctagcacttg ctcaatacaa gcaaggtatg atcccagagg caattgaaaa
    36061 ttttagaaaa gcaattcagt ctggtggttc tggtcaaatt gcagaattgg ctcatggaca
    36121 tttaggatct attttttatt acagagaaga tttgaatgga gctgaacatc acttcagaca
    36181 ggctgttact ctcagaccca atgatgctcg ttatctttac aatctaggaa ttgtattaat
    36241 caaaaagaaa aacacagaag aagctgtttc ttttttacaa aaagcgttag atgcaggagc
    36301 aaccgatcca tctatctatc gttatatcgc agaatctttt gaagatttga aaatgcatga
    36361 caatgcgatt gcctctctag aaaaagcaat gcgtttaaga ccagacgaca ttgatacatt
    36421 ggttcagcta gcagacctct actacaacag aggcaatcta agtcaggcag aagaaacgtt
    36481 tcgcaaagta attcgaatta ctcatggtga ttctaacact gaatctgccc gtgtcaactt
    36541 aggtatcata ctcgatgata tggaaagata tggagaagcc atttttgaat ttgaaaaggc
    36601 aattgaatta aatccaaaaa atgaaaatgc atactataat ttagggattg cctataaaca
    36661 tgcagggcag cctactaagg cattggagag ctggcgaaaa gcatatgcaa tcaatccaaa
    36721 cgaaaataaa tataaagaag ccatcggtga ttattaccta gaaagcaact atttagatga
    36781 agctgctaga gaatatgaag atattgccaa ggcaaatcaa tacaattaca aagtccgatt
    36841 gaaactagcc gatgcttatt ttagaaagca acaatacgat cttgccgaga agaatctaat
    36901 ttttgtttta aaccaatcta agaatgcaga ggaaataaaa ttagctcata gaaagctagc
    36961 tcttgtttat tctgccggag attctaaaaa taagacaaaa gccaaagatg aagcctatag
    37021 aggctctcat attgatccag atgatatgga aggcagactt gttcttgcga aagtcctgat
    37081 agactccaat tcccttatgg atagagaaaa agcaattgat gaactaactg ccatcattcg
    37141 ttccgatgta aagccaaagc tagcagcaaa ggcttacaac tacctcgggg tttgtctata
    37201 caaaaacaaa gaatacaaga gagccttgca ggaatttcag aatgccattg atttagatcc
    37261 aaatctaaca gaagcatatg ataataaaag ggctgcaagg gctgagtatg aagcaaacct
    37321 acagagtaag caatcttcta tctattaatc ccttgactat ttttgagagc ctaaaaaact
    37381 gatagtgagc tgatttatct atggatgaat taaacttaga ttcaatgaaa gaacaaacta
    37441 cagttaaaaa aactagcttt tgggctccaa aacaaagctg gatatttatt ctcttaactc
    37501 tatccctgag ttttggaata ttcgcaactt cggagaaaga tgaactcaaa gagaatttat
    37561 tgggttctga aaaaaaacaa aaacaagcac tcctaaccat taaaaatgca aacagagaag
    37621 atttatttcc gattgtttcg gatgtacttc gaaaaacaag ttctgaaggt gagattcaag
    37681 gtttaatctt agatttatac ttttcgtatg gacctgaaat agaaaaatac aatcctaaaa
    37741 taatagaaga cttagagtgg gttttcgaga atacaaaaaa tgaaaccaat ctaattaaag
    37801 ttcttcatta cgcagctact tacaaagaaa aaaggctcat gtatcacata ctcgatttta
    37861 ttcgatatag agatgctacc gtgcgagaat tcacatttaa agcaatcgat agtttcaaag
    37921 atgatcgagc cctccccttt atactagaat taggaagttc cgaccaacct atcctacgtt
    37981 attactatct agaatctctg aattatatca atgacgagag agctagtatg cacgttgcca
    38041 aacttctcac ggatccaagc cccgcaattc gaagtgagtc cattgtagta attgaaaaac
    38101 taggtctcaa agaaaaatta aattctgttt tggcaatggc gactaatgat tcaaactacg
    38161 aagtcagaaa gacagctgtt gtgtctttaa aaaaccaacg cagtaaattc cctacaactg
    38221 tttatcaaaa gaccattttt gattcgaatc gagaagtgag agatgtaaca gtagatgcaa
    38281 ttactaccat caaagatcct tcttatgcaa agttcatatc agctgccatg gaaaaagaaa
    38341 atctatcccc gcttagattg aaaatggtag atagtctttt ggttttaaac aatcatggag
    38401 gtggttttgg tttatctgct gcacttaaaa atgataaaga tagtgaagtc agagcgaaag
    38461 cagcactagc aattggtaaa ctaaaagcaa caccagtcgt aagagattta gtgtcctctc
    38521 ttgaccaaga aaaagttgta aatgtaaaaa ttgaaattac tagatctcta ggatcgctga
    38581 aagaaaaatc agcagttcca gttatcctaa agaaattaca aaccaatgaa gcaaatgagc
    38641 ttagatctga attcctaaca gctttgaatc aaatagatga tccgaaagta atgccagtta
    38701 tatttgattt gatcgatcta gagaatacag agattattaa aacaggtatg aaatctctac
    38761 taagaacaat gctttataga tatcatggcg gtggaaaaga atatagaact ttgattctat
    38821 ctgataagat ttctatgaat taactctttt tttcgtagat atcatccact ttcatattca
    38881 agttaaaaag tgctttctct gcaataatca gtttccagat aacagttcta agtaagctaa
    38941 ataaaaaccc agggctgatg tgcacaatct tcattagaat gtccttatca attctaacca
    39001 cttttgcctt ttctgaaaca gtaatcatgg tttgagatct tggtgcttca cttacgatag
    39061 aggtttctcc aaaaaagtca cctttttcgt aacttgtaat cttcacattt ttcttatggt
    39121 attttttgat aacagcgatc gtgccttcat aaataaaata catagcacca tcggactttt
    39181 ccccttctga ataaacctta tcccctcgaa tgagaacacg actcgttaga ttgtgcacgt
    39241 aattaaatac attgatattg tctaccctgc tgtcattcaa acgactagca agagctggat
    39301 ctaattctgg taatgcctta atgattttat caatcgcgga ctcggcacga atcaaccgtg
    39361 cgatagtcgc ctttagtata gaaaacatca agccgggact ttcttttgtt ttttgaataa
    39421 atttttcttt gctgaagaga agtaatttac cagtttctga tttaactgcg atacttgcag
    39481 ttctcggttg acttgatata agagatagtt ctccaaagaa atctccggat tttaaaacat
    39541 tgacttcttc aacactatct cccctatttt ttaaaacaga aaaagatcct tctaaaataa
    39601 aatacatggt ttcattggaa ggatctcctt ccttgatgat aaattcttta ttttgataaa
    39661 ctttaacgtc agtagaagca ggtgcttctt tgatcatttt agattttaaa ttaaatgttg
    39721 ccttttcagt attgatcaat ttccaaatag tatttttgag attattaaat aaaaactcag
    39781 ggtgtaaatt tacaattttt aaaaatgcta ctttatcaat ttgaactaac tttacatcat
    39841 ctgatttagt aatcgcagag agactataat gagtatcact aataaaggat gtttctccaa
    39901 aaaaatgacc aagttctaat gttgcaagtt ctacattttt accatggtgc tcttttaaaa
    39961 gagatatgct ccctcgcaaa ataaaataca tataaggatt tgcatccttg ccttctgtaa
    40021 aaatttttaa attttttcct aaattcaaaa taggtagaga atgaatgtaa tttaacgctt
    40081 taatattgac tagctttaca tgattctgat tgagcttgat aattaaatca ggcttcatat
    40141 tgggaattgc tttgattgtg ttttcaaaat ttgcatctgc acgcaagaag cgagcaatcg
    40201 ctgcttttaa tatagaaaac attaaaactg gattcgaaac agtctgttga ataaatttct
    40261 ttttactgaa taaaaggagt ttagcagaat cagagtctac aatgacagtg gcagttctag
    40321 gttgattgga tatcagtgca atttcaccaa agaagtctcc agtttttaat tgattgatta
    40381 aaacgggagt atctccttgt tgtttgtata ccgcacaact tccatttagg ataaaataca
    40441 ttgtttcgtt agaaggcgca ccttcctcaa tgagtttttc ccctttttta aaatcttgaa
    40501 cgtcagtgtc ctgtaaaaat tcgtttgtaa aaagtttatt taacatttta ttagagttaa
    40561 tctagaatag catctaccaa aatcaatcaa ttatttgagg aatactaaga tttatctatt
    40621 tttacatggt tctaagggat aatccttgac aattctattt atatgtaaat catctctgaa
    40681 agtctggtaa tttttcggag tacggattga tacaagcaat caaaaaccta gagctaaaat
    40741 ccttttttga atctagtttc aataagctaa aaaaccgaga ccttgcaatt caactcgcgg
    40801 atgtccaaaa gagatttcag gacttaacgc tttttcaatc ttttctacaa ttcttctcta
    40861 acttcacact catgttaaag ctagtaagcc gctcctctgc tagtgtaaat caaaatatga
    40921 aacaaattgt ttcagaaaca gcaagtttaa acaaagagct aagcgcaatc cgaaacataa
    40981 tggatgatta tttagaaaaa atacatcaat tttcagaaga aatgggaaat atcaccagtg
    41041 aagtttcaga aattcatttt caaactagaa acatggcaga gaaaaataac aacattttag
    41101 aatcctcgaa tgatatccaa agaaaagttc aaaatggtgc gaagacgatg gatgcaggtg
    41161 tttatttaat ttctgaatta gtagaacaaa acaaagaact aaatgaaacc attcgggatt
    41221 tatggtctaa atatactttc ttaatcgctc attctaaaga attagtaaag atttcagaaa
    41281 gcacaaaaat gctagcactc aatgcagaga tcgaatcagc tcatgcaggg gaagcaggga
    41341 aaggatttgc gatagtcgct caagagatgg gtagacttgc caaaaaaaat tcagattatt
    41401 ctagagagat tgcgaaaggg attcaggata tgcaagaaca agcaaagata actgaaatca
    41461 atgtttcctc ctctgtagaa ttagcacaaa attcagaaaa ggaaatacaa gaggcacata
    41521 aaacatataa aaatgtgagt gattcaatca cgggcgtttt aaaggactca actgcatttt
    41581 taaattcttt tactcattta gaaactagca tgaaattgat taaggaaaat ttcaattctg
    41641 caaatttact actgagtgag tcgaataaaa aagtaacaga aatatcaaaa gctatagact
    41701 cgcaaattaa tactatcgaa aaaatcgact ctaggactat ctcaactttc acagctagca
    41761 gagttttaaa ttcattaatt tcccaattta ctattcctgg atttaaagat aataatccaa
    41821 aagtaaaaac cattgaaaaa ttaattgaat ctattttaaa tataagagga attttaatta
    41881 cattactgtt tgatgaaact aaatccttta ccgatgattt aaaaaaagaa aaaattgaaa
    41941 tggattcaat catcaatgaa gaaatcaaaa tgctgcaatc attactctca tcagaactag
    42001 acaaaaacaa attcagtgag ttaagtcttt ggtggaaaga atttcaaaga caaacagatc
    42061 gaattttaga aaaactcagt caatttgaat ctggctccgc aaaagaaatc tatacttcta
    42121 taaaagacca aatcagccct acccttacaa ttctcgttag ttttattgta caagatataa
    42181 tcgatttaaa tgataagtaa tagaaatgtt aatcacaaga ctctgtaaaa tattcaatga
    42241 aaactcactg aagtatgcaa tcgctggagg ttttgctgtc gccttgcatg gagctgtccg
    42301 aggaactatt gacgttgata tagtcttaga tctaaatgaa aaaaatctaa ccctatgtga
    42361 atcgatttta aaatctatgg gatttgtaag taaaattcca attgatccag ctattctttt
    42421 aaaaaataga gaaatgctca tgcaagaaaa aaacctaatt gcatggtcat tctacaaccc
    42481 taaagatcct agtgaagtca ttgatatatt aatacataaa gatttagcag atatagattc
    42541 taccgttatg cgatatgcag atatcgaact tagtgtaatt gcaaagcatg atttaattgc
    42601 aatgaaaaaa gaaagtggta gaccgcagga cctagaagat gtcaaagcac ttgaaagtat
    42661 atgagaaggc ctgtccaata cttttcagaa gaatatttag atctatgtaa aaaattgagt
    42721 gtagaacaaa tcgttcaatt tgtagaaggt tttaggctcc tattcggaga aacagcaaac
    42781 cagccaaaat taaagcaaca agagaaaatt tatctggaac tagatattac tgataaaaaa
    42841 tagaaaagag tcaatggaaa catttttatt attttttgtt tcctctattc gttcttaaaa
    42901 tattctaaca gaatgagtgt aaaatctttt tttattcttt ttattgtttg ctttttctct
    42961 gaattgattg cggttacaat cgaaacaaat actggtaagg ttttcgaatc agttcaaatt
    43021 atggaagaaa cagagtcaat catagaattt tctgactcgc acggggtaat cacacgtatc
    43081 cgcaggagta gtattaagaa aattagcgag gacaaaccac aagagacatt agaagaaaat
    43141 gcagatgaaa gcctacctac ccccatgccg attcaaccta aaaaatcaga agttccagaa
    43201 cattcttttt tactcaatca atccatttct aatgacggtg cattccaagg tgctagttta
    43261 tttggagaaa gacaatctag gcgaaacaat cagagctata aaagtttttc acaggcttgg
    43321 tttctaactt caagtgtaga actactcggg ttacccaaac aatttcaaat atcttttact
    43381 accatgaacc cacttgtaga tagaaccaat acagattcag atttgtattt tcaatctaca
    43441 ccgagcggag ccaatcaaaa caaactagtt tcaaactcta tcaattctgg aacattgcaa
    43501 tatgatccca atcaagtaaa acctaggaaa gaaaaaaatg ccttaacaga ttatctattt
    43561 actaggctaa actatgagca tactacaaag cttggatcct ttggagttgg ttttattttt
    43621 atcaatgtaa acgatcccgc ttatgtaatg cgcggttatt ttacagtgtc ttggaaacca
    43681 cctgttcttc aatttttaaa tcctgttctt tcaataaaca ataaaatgtt atctgaatac
    43741 ggtggtattt ttcaaggaga tcacaactat cgactttcca ttggacatga atttttcaaa
    43801 gagcggctaa ttcatgttac tcccagtctt gttgttggat accaagatgt aaacaacaat
    43861 caaaatatga aaaaaggaat cagcgatata agccctagat tgcagtttaa tataagtaat
    43921 ttttatgtag cgttaaatta tatgtataga gcaactcctg ctcttgcgga tactaaaaat
    43981 tatactccgg acattggagt ctatccagac tctaaccaaa acgatggaaa gacaattgat
    44041 ccatccaaag cctatgggta taaaaacaat tttataacag attccattgc tgctaactct
    44101 cccaatgatt tagtccgcca tgtattaaca gatcgatatc aacaacaaca tattgtaaga
    44161 ggtattttct tttttaatat tgggtatacg gtgaggattt aagctaataa taattattag
    44221 caagagcttt cgttattctt tctttttagg aaactttaag aatacgaaaa ctatcaaaac
    44281 gaaattctcc agacaaagca gaccaagtaa caaattactt gtatcacttt tagaactaat
    44341 catttctgat aaagaaatat attcgctagt ggcttgctga gttgattgct ggtttatcgt
    44401 ttgctcattt acagtttctg tagaaggtgt attctcttta ggtctctctt ccgcaatctt
    44461 taccttttta atcatttctt ggtatttcat ttttaccatt ggatttgacg gataacgatc
    44521 tacggctttt tgataaattt ccatcgcttt tttatactgc ccggaattgt aataagtttc
    44581 tccttctatt atccataact cagtctggta taagttcaac gtcgggtcat tctcatttcc
    44641 ctgcatcgca atgtttaatt cttccctggc tttggcaagt tgtctagtct gaattaattg
    44701 tttaatatga gaattagttg tatcttgcgc gtgaagcggt agttgaaata aaaataactg
    44761 gaaaacatat aataatgcac taaaaaatat tctaaacata attcatcctt acctatttat
    44821 taaaattgca gtatgaccaa atcttggtac gggcatcgta gtagttgtta cagtattcca
    44881 aggacccgtt gacgggttcg acaagtctat ccaataaact gttgacgttg gaacattaac
    44941 gattgtagcc ccaccaaaaa catataatct tcttaaatca taagaaactt cagccgcagg
    45001 agcatataca cttacaggta gactcggtcc tgtttgaacg gtgtttaacg cagaaggagt
    45061 taaataatag tcaaatctat ttgatgcaag tagtgcagtg ggtggttgaa ccgtattgct
    45121 tagtgttgtt ccgcctgcca gaaaaatacc gggtgaatca gaaggatagg catccgttga
    45181 tttaggacga tagcaagtag aggatgttcc aaatcgcgcc aaactaaaat taccctctgt
    45241 aatgcccgtt gttgaattgg cagatggaat gtatgcatcg ctcgtaaatt gagttgttcc
    45301 agttgtatct ctacctgaat taaaaaagat agttccatct atcgcacagc cagacaagtc
    45361 agaacgggct gctattgatg aaattgaagt tatagtcgcc catgatccat ttccgctatc
    45421 aggtgtaaat ttatatacta tattaggtat agctgatgcg gaagaattcg atatactccc
    45481 accaattaaa taaatagaat ttccagaagg cgatgcaagc gctccctgga ttgctaccgg
    45541 aatggatgac aaagttttcc aggaattggt atatgggctg tattcctcta ctacattaga
    45601 aatagtagta ttcccactct ggtctattaa cattccgcct atcacgtata ttttattact
    45661 aagagtggct attcctgaat aacttcttgg agtaggaacg atagttacgg aggaatacca
    45721 agtatcagta accggatcgt ataaatcaat ttgagaaaca ggtgtattag tgctaccaac
    45781 tccgcctaat atccatatac cacgattcat cacaacttgt gggattggta cagattttgt
    45841 agtaaatttc tgagggatat aaactgcttt tctttcatca aagcatccaa cctgataatt
    45901 atgggttttc cccgatgcaa gatttaccag cttgtaatac tgaatctttt gcttaaagaa
    45961 aagaatttgc gtattttcaa atccaatatt tccataggta agtattccgt aagaagcaga
    46021 agagcattcc caaactaagt ctaccgtcgt ttctgtagca gaccgaacga aactgtaatt
    46081 gacttttagt ttatcgctct catttccctt ttggattgga ttttctgaac aggaaaaaat
    46141 caaaattata atcaaaaaaa gtttcataat gaataaacta tccctatctc aaatccaaca
    46201 ttatgataat atggcttttg aaaatattga agaacacgag gacctattcg aagcgtaagt
    46261 ttcccaaact taattccctg gtaaccaaag ctggttgcag ataatccttg aaacccttct
    46321 atatacgcct cgtctttgcg atagcgaatg tatgctccac cgaaaccaat atttgcatag
    46381 aaatctaacc atttggaaaa ggattttcta tattgcaaat atactatttg gttataaaat
    46441 ccatatcttg aaccttctcc agaagatgta agatattcag atttaaatcc aatattccaa
    46501 gtatctttcc aactcaaatt agaaggctcc aaaaaaacac ttcctccata aattgtggga
    46561 ctaatagcgg ataacggttg cccatttgag gcaaaatatg tattaacccc aaatcgaatg
    46621 ggagtaagcc tatacgactg ctctagataa tcttttggaa ttgtaacttc tattttagga
    46681 tcaggctttt tcacatcagc aatctttgct ttatctaaac tcaaataacc taaaatagtt
    46741 tgaacagtta aaattgtatc tgattcttct gcgatatttc ctttcaccac tgtagcatct
    46801 aacatcgtga ttgtcgctaa cgtatatcct ttatgtgttc ctgttttgct attatcccgc
    46861 atttggattt ctgatttagg aatttcgtat agtttatcct tgaacctaat cttgattatc
    46921 cggtcattct cctctactgt gtcacagatg aatgcatcgc cggattttag gatgagttcg
    46981 agggaataca ttggttgaac aaagagtaac aggaaaagga ttctttttat catttgaatt
    47041 agtaatataa tactctattg ttactagtat ctacaacata taagcgttcg cttgcgtcta
    47101 acgcaagacc tgccgcacca cttaagctat tagctgaaat acctccgtta tttacagtcg
    47161 ttgtagtaaa acttccgcct tgaccgtaga cttttgttgc gatggtactg ccactcggaa
    47221 agtaaataac gcgactactg ccggatacgt agagacctcc gctcttatca atcaccaaat
    47281 aattggctcc atttacgcta cttgttgtag cagctggata tattgaagtt ctggaagtaa
    47341 aatcagactg tccatatact ttagaaggaa ctatcgaacc cgaaggaaaa tataatattc
    47401 tattattgcc gctatctgca atatagagtc cgttgcttga atctaccagt agtccggtag
    47461 gattataaag agaatccgct gatattgtaa atctatagtc tatagagcta gtaaaacttc
    47521 ccaatgtccc gtagacacgc gtggctgtag tgcttccgga tggataataa agaactcgat
    47581 cattacctga atctgaaata tacagtccat tgcttgaatc taatgccaaa ccagtaggtc
    47641 ctccgagact attcgctgtt acccctccag tatttgctgt ctgagttgta aaaactccta
    47701 attgcccgta gactcgtgat gcggtagtac taccggaagt ataatacaaa accctgctgc
    47761 cacaggaaat gtaaacacct ccagtcgaat caagtagtat cgcccttgga gcagataacg
    47821 tcgatgccgt aacattacct gtgccattag aatcggaagt gaaacttcca ttttgtccat
    47881 acactcttgt cgctgttgtg cttccggtag gataatataa tactcgatta ttaaacctat
    47941 ctgctatata tactccccca ctagagtcaa gcgtaatccc ttctgggtta tataaactat
    48001 ccgcagagat tcctccttta ttggaagtag ctgttgtaaa actccccgct tgcccataga
    48061 ctctcgttgc agtaggtcca cctatgcctg tactcgatgt ggtcttactt tgcaaaaata
    48121 aaagccctgc gactaatatg tttttgtctt tgctattgga tgactttgtg caagcagcta
    48181 ggcttgttag aatgattaga atgtttgtcg ttttgtaaaa tgggttcatg ggttactcct
    48241 tctttgcttc ttctgtatga tcgtagagaa taaattcttt ttttacttgt tcgtaggtct
    48301 tgcctacatc atccttccca tcaaacgcaa acttggtatt aggcggtagt tctttgaggg
    48361 taatggtaaa accggggaca gtgttcatag tttcaagagt atctaaactt gttgcattat
    48421 aggaaccacg cggaagagcg gaattaggat taatcgctat ctctatttgg taaattttgc
    48481 ctgggttaaa gttaacatat acttgcaaac ctctatgaat gaattgatcg aaataaagtt
    48541 ttgggacttt atattctaag tttttatttc gctttcttgt ataatacatt acttcgtctt
    48601 ccgtataatc aaaatagaga ataacagtca atagaccttc ctttgcagca ataggaaata
    48661 aatactctga atccattgcc ataaaaatac aatttttttc tttaattgcc aaatgttcac
    48721 ttattatttg ttcttttaac ctagatctta cttgataaaa tttattttca tctttgcgag
    48781 gatttagcag agaattccca aattgtatga gtaccggtag catctttact tttcgatcat
    48841 aggtgcctaa atcatagtca aggtattcgt tgcctagact aggtactttt aaataaagaa
    48901 aagcatgctt atcatttaac cggattggta tcgaattgct tctcgccata caattttcta
    48961 gactttttgc tcgattagtc tcataagtat ttctttcgta tgaatcgcaa cttaccctac
    49021 cactagttgc atataaatta ctatgtataa aaagaacaag tagtagcact gaagttctag
    49081 gattggataa tttacatttc attaatatga cccaagtgtc tgaggctttc gcaaagcagc
    49141 ccaagcagta ctatcgaatg tattctgaaa aatagttccc attatttttt ctgtgaaata
    49201 aaaggaacca ctccctacta tttctgcccc cattctatca aaagaatggt atgttggtcc
    49261 attgagtatg caatcattac caaatgtggt tgcatcccaa agcccgccgg ctaatgaact
    49321 ggaggaactc aaaaaatctc cagccgcttg tccgcgatat gctcccctct cattataacc
    49381 atgcttctgc tgaatttgtc gttgataagg attattgcta gtatttatag gaacccaatg
    49441 tcctccaata ttgcgaccta aggattttgc aagttctccg aacaatcttc caatagtaaa
    49501 atttagagta tatgtcaaaa tccatgcgat agcagggtgt aatcctagaa aagcgatacc
    49561 tgcttcaatg gctcctccta catatgcacc aatcacaaat gctccaacaa ctacccaagc
    49621 tgctatagta attgccgttc ctatcttaaa acctaactcc gcgccggcca gtgcaccttt
    49681 taatgtccct gtggcataat acccatgaac aaaccccact ccagcaccta ctacgcctaa
    49741 cgcatctaca aagactgcaa aacgaacggc tgccaccact gcactcgctg caaatgcagc
    49801 cgtagcaata gttgctgttt gtgccattgc tccagccgtc atagcgtaat tagctacagc
    49861 agccattgcg gtatatactg ctaatcctgc cgctattgtt acagcagcgg ttatagcgat
    49921 tgctgttacc gctacagcat aagctgccat cagtaacggc actaaaaaat tcccactcgc
    49981 atctccatat cttactggat tcccttccgt atacatataa tgattcaatc caaaagttga
    50041 tgtcgcattc gtcacattat ccgcactcgt gaatgaacct agcactggat cataatatct
    50101 agcgttataa taatataacc ccgtgtcctg gtcatcctgt tgactcgtat atttgtatcg
    50161 gaatatatca ggaccatagc tattcatacg at
//
LOCUS       DAOQOS010000029        50101 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_266_length_50101_cov_6.474104, whole genome shotgun sequence.
ACCESSION   DAOQOS010000029 DAOQOS010000000
VERSION     DAOQOS010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 50101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 50101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50101
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_266_length_50101_cov_6.474104"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            190..1326
                     /locus_tag="PLF66_08380"
     CDS             190..1326
                     /locus_tag="PLF66_08380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64094.1"
                     /translation="MIPEEFETKSVLSDDDMQIILSAFRILEFPNYNINPRDVDYAFL
                     YQVEKLERIFYREPIELQRRKDHLLEQCRICVSAAKRPKLFEEIIEYKKKEIKADSHF
                     IPFKHYKEFFLNLWSDLKIFQRRLVDKPLKFINHILVFLWSLGKNLIILSLKNGWEFL
                     YSFFTSPRFLHWVNQNWKTRLITNLFATIISFIMLFIPIYIIKLAYNNVREFWNDQLT
                     NVENLPSEDLIGYIPKNYIIVKDKLGGEELLAIPFGTEIKYRKVNISMDEFAYYIPSI
                     DSYIFNEELANSVPSIVASKVFTLIEEENQYIPLQNYTKKLPAKKIELFSGNFRIFDS
                     TGKREYEGTYSINKGYISLSSQSLNGKLKYNHDGSFYFSTTVFK"
     gene            1494..1859
                     /locus_tag="PLF66_08385"
     CDS             1494..1859
                     /locus_tag="PLF66_08385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64095.1"
                     /translation="MENFDPNQLDMRWYATSKEDRLDLLYDLVKQVFRKITELEDEMN
                     EQQQKVLDAISNLQTSQDENFKKLKETIPILELLKIRTRLQKNILASLNSGFNLTGTN
                     VEQALVALNELDEALKQWT"
     gene            complement(2368..2865)
                     /locus_tag="PLF66_08390"
     CDS             complement(2368..2865)
                     /locus_tag="PLF66_08390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64096.1"
                     /translation="MAFLPILVIFGIVGYLVYMGAFLPVPIEERNVGPFHFIYREING
                     KDFSLVGNTTREIGELLKQYNFSNVKSFQIFYPEEEKKLSEVGFIVLETVQDLDGVKI
                     KSIPENLCMTTTFPFRSSFSFVLGFMKVDPALKFYRQKNSYKKTEAMVCLDQDTIQYM
                     QPIVK"
     gene            complement(2876..4291)
                     /locus_tag="PLF66_08395"
     CDS             complement(2876..4291)
                     /locus_tag="PLF66_08395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64097.1"
                     /translation="MNQYTALIPLLYPLNLDGNAWFPNLYKNVILSIFLDNPGRAFGS
                     DELIKYLSTNYSLKVQNVPEFEEILSMLSKEDQLILPQGEKYLINPSKQQEVEDYYTK
                     VIGIDEQEDGNTKLEFESILKEITAVDESGLLWNSFYNELFLPLVYHFYIRLTNPTYK
                     TEKELLQSYKSYINRFLQNKDEVHELTTNFLIKTSTSFFKKHLIACIFYESVNGEMKN
                     YDSILKLLDHPDNLDLFLERNMIYFMEIMLTMYSIEERLKSSTASIASLLFEDSFNRN
                     LHYTDITNKQNDFFKKNLRAESSTILENVQNENAALKTNLNTVQNDLSNVNSQIENLK
                     ENFNKQSKYIFKIQSTIEEKFKEHTENSINNLKNRNVEIINKITELSMIFVPATIILL
                     SAVFYLLPAYILPAAFGIGIVYLVRVAFHLFEFQLDKKLIEYFKNELKHFEKNVPTSS
                     QEVVNTQPQTTSELQAVEVTK"
     gene            complement(4368..6122)
                     /locus_tag="PLF66_08400"
     CDS             complement(4368..6122)
                     /locus_tag="PLF66_08400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64098.1"
                     /translation="MILKRDKIQNRFIGLGWLFIAGFIFWVNAFSIFSNHRENKARHP
                     SGIDYVTEIPARTSFGDDLITEEEFHDPLYPHRRIPIIGRGIFASPENDGQVDEDNIT
                     SPRARLEKDFLNQGFEEDLSMLAGEAHFGNCLNGIFTMNYDGFNCCCAYATESCQGLA
                     RISFGFPALPIDENGQRGKLPGYVRAVIDARWFRTTYPNTQNKNRSLNKSVDFNSGPF
                     QSGIKFLPNYLQWRYIINKDFDPDSEQPEGLKNLISYCKGNVAFDPSVGGGEDNIRKS
                     LIPNPSDPLSKDFRAFLPKDTPNGRPDWTPNPRTPYDTCVRPYDTKDVAGRRVYSSDL
                     HVAYEMAEDYCYNKIPPVPHMSSNGLFKDKLGRHARWNNWADRYETHGVDSKTNEAFD
                     HRFKIKHSNTFFHYRASLPYANRYGYEFNCVLEVARPFELEVRHVDPCPPMVKYSDLG
                     KGYDPKNCVQSEGYEYQDTHSAFGPVYIVNDAKAPNSLRLATNSEYPPGKHPSFFGEK
                     GMPNMNCVDAKRKCLRHDRWTKKCNDDGTPSGDSSVIFPPEHEAKANAWENHNIPAHD
                     HGKCKPLEWDFFAKQYIR"
     gene            complement(6610..7086)
                     /locus_tag="PLF66_08405"
     CDS             complement(6610..7086)
                     /locus_tag="PLF66_08405"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HMZ64099.1"
                     /translation="MEKIDIKIDIKALIEKVFDVLSDHEKYSKFNGILSSKLIQWGEY
                     EKNGKGAIRQLDSLGGTILEEILAFKRNDFFEYKVIQCYVNLGLIRIDIPLVHLLGRV
                     NFKETEEGTEINWVSVFDLDIPFLREEITKIISYTSGNAFYFLLQQLKFNLELNRY"
     gene            7394..8656
                     /locus_tag="PLF66_08410"
     CDS             7394..8656
                     /locus_tag="PLF66_08410"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HMZ64100.1"
                     /translation="MSSIGINPKTSSEYKVVIADSSNIERKLLARFLTLNSFKITNEA
                     DSTESILHNLESLDPKPDIIFIDTTILGKEGGQFLKILNSNFPEIKVIFIISSKVQEA
                     QMLKNKINYYIMKPFLKKNIEEKLYEILSDKPMEINSEDKGDVHLKKVFIPPIPNAVH
                     KILLFEANTTSGSNELEELLYPDKSLCADLLRVANSSLYGRNGSIKALHDAITLLGIK
                     TIKNIVIVQARRHMTGNLLKESIFKKFLYEFSIFNSLISHDLISLFGKAREYKEIFLL
                     SSFRKMGMNILALNFPDKYAKILKAFEKGEGDLIALEKSELSLDHVQVGVSVMEEWKM
                     PIIFTETVRDQTFKPSNFKDVGDIDRVTRMAEVLTKHLMKITLSELDNQILEEADHFY
                     NPKDHFIALFGEDYLANITSHPFFEIPI"
     gene            complement(9097..9900)
                     /locus_tag="PLF66_08415"
     CDS             complement(9097..9900)
                     /locus_tag="PLF66_08415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64101.1"
                     /translation="MNSEFSGSNASTQIGLTNKNWLFSYTYAKMLMNLSPEGNNDLPL
                     IYFNGKNLQNIYNSRIDYNWFITSKYAINFSGRQFVRSGTYTWEKVIDSPEYRTEFIA
                     FPRVEVNDKMRINMFSLGPKIPITETLEIEPYFQYRSVNYYLNVFPSLGATTVGNGFI
                     PTNPDDILKAWTYQNLGTAYNFNLYSPKVLNFGNVGLKILYKPVPFIILRGDIRRNYV
                     LAAWEIRGLLTFMFSKYFGITIGGVYAQKEVNPMIMKAWEIGPMIVYSF"
     gene            complement(10495..13026)
                     /locus_tag="PLF66_08420"
     CDS             complement(10495..13026)
                     /locus_tag="PLF66_08420"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HMZ64102.1"
                     /translation="MNTINKKTILLVEDELLIAFMEKQQLENEGYEVLHVSSGEQALS
                     TINEKGQSIDLILMDIDLGVGLDGTQTATEILKEHDIPILFLSSRVEKEIVQKTEKIS
                     SYGYVVKNSGITVLDASIKMAFKLYEANKTTQRQKEQIGAILYSIGDAVISTDKHGNI
                     TSMNPIAEQLTGWLFSEANGKHLDDIFKIINAHSREPVQSPVNVVLSTGKIVGLANHT
                     ILVSKTGKEFQIADSGSPIRNQNGSIIGVVLVFRDVTKEYAMLTQIEESEKKYSILFN
                     KSGIPTVLTKLPENQFVDVNESFENIFGYTKNEVVGKTSVEMGIVKKIDQVETEYHVN
                     SPQKSYTGEREITTKSGESKFVTIHVSKVQLKNNDYAITTIYDITDRKKYEKLISNQA
                     NMLANVNDAIIATDSEFRITYWNASAVKIYGWKEEEALGQYAPSLLKTIYIGKERSDV
                     VKSVLDKGKFFDVFIQSRKDGTQFYMEGNIVLLRDESLNVIGYISVNRDVTNRIKLEE
                     KQKASEARLNGIVESAMDAIISLNQDSKVLLFNTAAEKMFGYKSEEVLNKNLDVLIPQ
                     RYKEKYPDSVEIFLRLGEVKRKKGQVIEMVGLRSNGEEFFIEVSISKINLGEEKIITA
                     ILRDITVRKNSEQKIINLLNEKEIILKEVHHRIKNNMLTILGLLTIQSSRLENQTIKN
                     ILLDAAGRVKSMMILYDKLYNTEDNGNISLKTYLPSFIQEIVNIFYVINPVQIETKID
                     DIILTPTILSPLGIIFNEWITNTIKYNSTKGQISIRVSAERLDNHIRITYEDNGSGLP
                     EGISYENSTGFGLQLIHMLAHQINGSVFIDQKNRSKNILEIPLSK"
     gene            complement(13010..13216)
                     /locus_tag="PLF66_08425"
     CDS             complement(13010..13216)
                     /locus_tag="PLF66_08425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64103.1"
                     /translation="MIYLVFGITNELRKSKERFFARLISNNACLTLFLFKLYHFFYKV
                     FLTPKESDESVNILWGFTFYEHNQ"
     gene            13940..15826
                     /locus_tag="PLF66_08430"
     CDS             13940..15826
                     /locus_tag="PLF66_08430"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HMZ64104.1"
                     /translation="MNFFKYLQKSIKNLINTLFIFVVGLLTVFGLLLIRSYVMSYLSS
                     YRILENARNVGQLNLGTIELSLERSVSQVTLNIPSPITVNFQSLIRKQRDLGTPKIES
                     VIKHLSESGNNPKAADRLKSSLSELNDLRKTVDKNLMLPIEEREKEFIQKFHILFPNV
                     IEEIQSNKFALQSEDSVLPTKVQLLEAITQYAWEVREFGGRERTYFAIALFNKQPISS
                     ELLERMTMLSNRAEYAWKNIQIMKQHPDLNQEVLNEIVHLERAYFSDYYLIRERLKQE
                     AIKGNYTMEFGDFFEKSSAALGNAESLAKLSVDTVILHAEKEKQKAVVYLITISFFTI
                     FALVLCIYLLHYLNQKVVGNIIRVTNILDSLSKGNKDINYEGFHDLENEIGSMVESSK
                     VFRENLLRLEKLMVEQSSAVNETTQVVEMLDKSSKMSAEQAGQVSEISQLVLKAANEG
                     EQMASQMKSLQAEIQDKAITINDCIKELEIQTNQIGSITDVVSELAKQTNMLALNAAV
                     EASRAGEFGRGFSVVSIEIRKLSDESKNSAHRIQGLIDEIKKSVRKTVSLTENGKQAA
                     ITGSKYAEDTSLKFKDVASSIGKLSDNMSSIAINLRQQVRAHEEVLVAMNSLNNKTRQ
                     LVQI"
     gene            complement(16050..17081)
                     /locus_tag="PLF66_08435"
     CDS             complement(16050..17081)
                     /locus_tag="PLF66_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020768827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HMZ64105.1"
                     /translation="MKRLIFRKRNVLEWEEVEEPKITEQDQAIVKPITIARCDLDLAI
                     FRGQTFFRPPFPVGHEFVGEILEVSENLKEKFKKGDRVIIPFQISCGKCESCLASDSK
                     SCDTMHSHPSDFGMGSTAKKMGGAVAEQLLIPYASHMLMPIKKEINPISIASISDNIV
                     EAWKMVGIHTANSDINSILVQGGYAPSIGLYSALLGKAMGKDVTYSDFSQERLSIANN
                     AGIKTLEINEYSGSLGKFDLVADASGVKEGFYSAIKSVRSYGKVTSASVFFTNSFPIP
                     FLEIYDKGVEFTIGRVNSFEWIPKVLEKIEQGNFPVEKVVTQIADFSDAKEAMLEETT
                     KLVIKNINT"
     gene            17206..17778
                     /locus_tag="PLF66_08440"
     CDS             17206..17778
                     /locus_tag="PLF66_08440"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HMZ64106.1"
                     /translation="MENPKERIINTSIKLFYSQGYDFTSINQIIDESGTHKASFYRYF
                     SDKSDIGLEYLYLKGEEIRTLIFKLTEKSSSIEEFVKSWAALLKKQSKSGSYFGCPLV
                     RFISGSSIQDEKLREKIRETLKSWMLEIELFCEKLKNRKELKANFQSKQFSKLVVKLF
                     QGNSSVFVITNDLSTWDDFKNEVTASIHNF"
     gene            18126..18665
                     /locus_tag="PLF66_08445"
     CDS             18126..18665
                     /locus_tag="PLF66_08445"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HMZ64107.1"
                     /translation="MIATMEKINEKEKRPRSEKLVRFQDCDPLGHLNNAKYIHYFMNA
                     REDHMVEYYGENLYEIGGKSNGGWVVAKNEISYLYPAIANERLIIETALISLGKKNMV
                     NEAVIYDKDRTHIKVVSWVHFTFVNKLGRPISHSEELQNFLESILDRDALFGIEDFDT
                     RIATLRKKVKEEKRNRYAI"
     gene            18655..19107
                     /locus_tag="PLF66_08450"
     CDS             18655..19107
                     /locus_tag="PLF66_08450"
                     /inference="COORDINATES: protein motif:HMM:NF013231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HMZ64108.1"
                     /translation="MPSKPNEKKINNQVRTIAKECLLMRTRTLSRAITSIYEKKLLKH
                     GLKASQLNILVTVANFSSIQQKRIGKFFHMEKSSLSRTLVPLEKNGWIRTEKIGVSQK
                     VFITPNGLKLLEKVFIDWQQAQKEVQLLIGPSMAMEMKDKFKLLNSIS"
     gene            19366..19725
                     /locus_tag="PLF66_08455"
     CDS             19366..19725
                     /locus_tag="PLF66_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020770344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HMZ64109.1"
                     /translation="MEKPKIFKTSFASVYPLYVQKAERKGRTKEEVNTIIYWLTGYNQ
                     KSLEKQMKKGVNFEQFFEEAPHLNENVSLIKGMICGYRVEEIEDKLMQKIRYLDKLID
                     ELAKGKSMEKILRAPQK"
     gene            20271..20456
                     /locus_tag="PLF66_08460"
     CDS             20271..20456
                     /locus_tag="PLF66_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002176753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64110.1"
                     /translation="MLSRYVNYRGIDIVLYLKDGTVVELDKNRKLDGDVVIKNGKEGI
                     VARIEISDIRKADFFAA"
     gene            20571..20780
                     /gene="rpsU"
                     /locus_tag="PLF66_08465"
     CDS             20571..20780
                     /gene="rpsU"
                     /locus_tag="PLF66_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002104827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HMZ64111.1"
                     /translation="MVGIVIKEGESIEGALRRFKRDCANAGILSEIKKREHFEKPSVI
                     RKKAIDTAKRKRDKKKRIFAKKEKI"
     gene            20850..21305
                     /locus_tag="PLF66_08470"
     CDS             20850..21305
                     /locus_tag="PLF66_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HMZ64112.1"
                     /translation="MSLQVRINEDLKSALKSKEEPKLSTLRLLKSDIQYELTKTGAST
                     LSDDQVIAVIRANSKKRRETAAEYRKANREDLALKEESEDAVLVSYLPASMPEAEIRE
                     VIAKVIQELSPKPSEVGKVIGKVMQEVKGKNADGSLVSNLVKSMMAEAS"
     gene            21314..23128
                     /gene="dnaG"
                     /locus_tag="PLF66_08475"
     CDS             21314..23128
                     /gene="dnaG"
                     /locus_tag="PLF66_08475"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HMZ64113.1"
                     /translation="MQTGTDFVSQVKQQINIDAYISRFVKLKKQGKRFSGLCPFHGEK
                     TPSFYVSPDLGVYHCFGCGKSGDLFKFVMDFERVDFNRAKEILSEYSGIPLKVSGHSD
                     LNEKNAMYALNQKFLEFYIQNLKSDQGRQAREYLKARKISESEIEYFKLGYSLPGFDN
                     WTKLSLSQPEIKIALKLGLLKENQNTPNRFYDFYRDRIMFPIFETSGKVCGFGGRRIQ
                     DSEEAKYINSPASLIYDKGKMFYNLSHAQNSIRKTRTAILVEGYLDVLGLFSREFDNV
                     VAPLGTALTDKQVRTIKNYADKVLILFDGDNAGKKAALRATEICIRENLPTEILLLEN
                     GKDPFDLSREKTRVEILEVFSKTITASDFVIQETIQNTNSQSKPEQKKKAVENLFQFI
                     KTLEKETDKQSYLAEGAKHLGLSFSALLNDFKKESIPNFEPQKDDNKYKPVAKKDLSP
                     AVKCERKLISMLILNDSLLHHIKDLQDLEFLDSESSILRDLIYNRYLDNQEISYESIF
                     QSEEIDEEILSVITPYMMEEVESKELSEEEKELIFKEALLQQRKFVIETEINKLSLSQ
                     NYIEDAKTNLEKLMILRKEKQAIVESIGSLSLAKKEVN"
     gene            23131..24882
                     /gene="rpoD"
                     /locus_tag="PLF66_08480"
     CDS             23131..24882
                     /gene="rpoD"
                     /locus_tag="PLF66_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010574820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HMZ64114.1"
                     /translation="MENLQSLPEVQRIIAIGKANSEVSYDEINEILPDKILNSEKIDD
                     VFTLLHELGIEVVEEYSKKAVEQPIVPPKEEKPTKKRKESSSISATSEDPIRLYLKEI
                     GKVSLISGETEVFLAKRIEKGEKIIEETILSSSILRANFAKLMPKIKSKKIKVYELVK
                     VDKLYALNQNEADKLEKVFFSNMDIIQNEEKVYNESVNRIKKYSENSKKYKELREKIE
                     ITSSKIDNATRTIGVSQREIQKISQKIKSMVFRIKEIDRHFLKIKARYGHDVKEIKTF
                     NRFIEKNENIEEIEKMMGIDIDEVREVIKDIRNNERKLRRMEQEAGSSTAEIKEWGEK
                     IMKGEREISQAKKELVKANLRLVVSIAKRYANRGMHFFDLIQEGNIGLIKAVDKFEYK
                     KGYKFSTYATWWIRQAITRAISDQARTIRVPVHMIEQVNKVIREARLFVQEFGRDPTN
                     EEIAERLGWPVQKVKSVKNVGREPISLEIPVGSEEDSELGDFIEDKEVESPLNSAAGS
                     ILAEQIRQVLHTLPAREQKVIRMRFGLDDGYAQTLEEVGYQFKVTRERIRQIEAKALR
                     RLRHPTRSKKLKDYILD"
     gene            25077..25784
                     /locus_tag="PLF66_08485"
     CDS             25077..25784
                     /locus_tag="PLF66_08485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64115.1"
                     /translation="MKNIFKNFKKSLVLLTKLPKAIFLFSILFLLNACFEYEETINFK
                     KGFSGFVEITYTVPLNHKSDNSVIKFLPIYEEDINRRINKGIFSKNLKIKDYSLKYLE
                     KNEKETNPLFQKKARVNYKIEFNDLSTLDGVLLGSLFVKKKSSNSISIKREFKSVLKP
                     IDQTSTTGEKKIISESTRLLGDGYISFRVNYPLASECRSNKGELSLGSLFYKIPLVDT
                     IEKPGPQSWDYTITTLY"
     gene            complement(25792..26988)
                     /locus_tag="PLF66_08490"
     CDS             complement(25792..26988)
                     /locus_tag="PLF66_08490"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HMZ64116.1"
                     /translation="MPTVVFAEDSAVQGVMLKRILLDEGYTVFWGKNGAQAYALVEKE
                     KPNLIITDVEMPDMNGFELCRKIKSNEALKDIPVVICSSLSRPEDIMTGIECGADGYV
                     TKPYDKKYLMYRVEALLSNPISTNEEVTPIHINYAGKTFEIVADRLHILNMLLSTYEN
                     SLKQYNELVNAQIELKKLNKSLDLSKKETEEVLLNILPSRVAKELKETGKTKPIFYET
                     ASILFTDFKGFTKLSEKFTTEELIHQLDQFFSYFDSIMQIYKIEKIKTIGDAYMCVSG
                     IPEANENHAINIVKAGLELADYMNIVNTLNSQKGIPPWEIRIGIHSGPLVAGVVGTKK
                     FCYDVWGDSVNIASRMESSGSPGRVNISGSTYELIKDNFNCEYRGKVAAKNKGEIDMY
                     FVDSQK"
     gene            complement(26990..28033)
                     /gene="cheB"
                     /locus_tag="PLF66_08495"
     CDS             complement(26990..28033)
                     /gene="cheB"
                     /locus_tag="PLF66_08495"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: protein motif:HMM:NF001965.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis-specific protein-glutamate
                     methyltransferase CheB"
                     /protein_id="HMZ64117.1"
                     /translation="MTDNKIKVLLVDDSPIVLNILKRILEKSPEVLVIGSAKNGAEGL
                     EQVNFLNPDVVCTDLMMPVMDGLEFTKKLMETNPKPILVISAALQGADETNVFQLLEA
                     GALDVFPKPAGGTDKEYDLIRDKLVSKIRIIKSIPVYRKIKKNPGETTASHPVVASQI
                     KSKINLLAIGASTGGPNAILSILEKLPRDISVPILCVQHISDGFIDSLVGWLGQNTKL
                     KVKIMQDGETPLPGTVYFPQNMKHMIIGPDGKLRVSNEPPFNGHCPSVNVMFKSVAKY
                     SGDKALAVLLTGMGDDGATGLLTIKHTGGKTIGEAESSCVVYGMPKVAKEIGAVDIQL
                     PVDKIAAHILGYM"
     gene            complement(28030..30495)
                     /locus_tag="PLF66_08500"
     CDS             complement(28030..30495)
                     /locus_tag="PLF66_08500"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HMZ64118.1"
                     /translation="MGTMIEDQELRDLFQIESEEHIQVIEKCLLSLEKNPQDYDMLHL
                     IFREAHSMKGASRMLGISDIESIAHILEEVLGKASRGEVQITSNHIDIYYEGLDAIKK
                     LVNEAVTGESSGIDVVTVLDRLLESKNNFAPVTDSKGGKPQVITTIEKKETQESKPKE
                     EIIQTPEKEIKSEEKIEEKPKLETPPNSVIKPNPPKEEPINSDIDKIIESKKASVVDT
                     PSAKKEIETDKNKQQVDSKKTDTMRVDSSKLDALMIQTGELIVTKNRILNRVKEVGDA
                     YYSYEELYRTVQETRNILSNFINQNDLSPKHKFFLNQLQEINTKQTEKLEILEENISQ
                     LRKNLSNDTTRLSMTSMKIERRIYNLRMLSLSNVFSLFHRTIRDLSRETGKNVQLEII
                     GGETTIDKQILEEIKDPLMHILRNAVDHGIEAKEERIKNGKPELATITLFGKSHSDTV
                     VIEIKDDGKGLDVEKIKNKALEKGLYSQEELNQMDQKRIFSIIFQHGFSTQSAVSNLS
                     GRGVGMDVVKNFVEKFKGDIETESELGKGTTFRIKLPIKFSTTHILIISVGNQKFGFP
                     TENIVLSKTIHPKDIFILEGRSTVIIEKQPVHLVQLSQYLEISQIKPKKLNAPTPCLF
                     IQSDGMRLALAVDAVIEKNEVIIKSFDGILKKVRNVSGATILDSGEICIVLNPKELMK
                     TITKTNMTVAVETLEIESKKIHALVVDDSLTTRVQIKRILESEGYIVEVGIDGEDGWT
                     KLKSGNFDLVVTDMEMPKLNGLEFTKRIKNSNTYAETPVIILTSLGSEENIKQGMDAG
                     ADAYLVKTKFDRKDLLQIVKRLK"
     gene            complement(30507..32465)
                     /locus_tag="PLF66_08505"
     CDS             complement(30507..32465)
                     /locus_tag="PLF66_08505"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HMZ64119.1"
                     /translation="MLLAFIHRMDFTARLIFFTVISTLTVALSISIPGHFIGKSIIYS
                     SANQQIELIRDVKKNEFLEYFEFKIRDLFIFRKSVAATKRFIEMRTAVERLGGLDRAK
                     DIYKSLYITNNPNPPGSKQNLLEADGSEYSRAHGQAVSFFSAIIKERNYYDAFLIDAK
                     TGNILFTVAKEDDYMTSLVYGPYKDSNLGKLYRKILNSPDGGQQAQFVDYEPYAASNN
                     QVNSFIGTGIFIDGRLEGILVFQLPLDEISNKIRRSLIKDRKEISYLVGGDYLLRTDY
                     EENTKDSVILKKKYDTDHVKKAFNGESGITVTKGITGIKSFSAYTPIELLGVRFALLI
                     DLDYDETLTSLSSFQTQMILVTLIVLIVMAGASFFLSRVLSRPIQESVNILSTSIREI
                     SSVIDNHERTANMQSASVNETTTTISNIGNSSRLSAEESQVVTMKAKDAQDLAVNGHN
                     AVMEMIHSIDSLKLKVSGIAEQILRLSEKTSQIGNIIGLVSELANETNMLALNAAVEA
                     ARAGENGKGFEVVAVEIRKLADESKKSALKIQEIISEIKKATDSTVMATEEGVKKADE
                     SSKLGSKVLDSLSGINHSVSAVFDSIEKISINIRQQSISMNEIVSAMNSINRGSQDSA
                     SGIAQTKVGVSQIGDATKKLKDLVEGEK"
     gene            complement(32476..33501)
                     /locus_tag="PLF66_08510"
     CDS             complement(32476..33501)
                     /locus_tag="PLF66_08510"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HMZ64120.1"
                     /translation="MAIEDKKLTNYLVFSVQANLYAISSACVVELIWLPELSRIESSS
                     FFVVGYFNFRSEFVPVIDLNLRQGIKTKRYSVQDRILVISTGNQKIGIHINDVHNVIH
                     LPFEKPDSGEDSDPERLTDGVIQTEFGITQIINIDALSNLNIPNADGKKIKSFTDIHL
                     AKLFGGLSEVDRQILISRRKNYSQSAEIKEYANLLSVVVIRLKDEFLALELKYIIEFA
                     HGEGITRIPCVPEHISGCINLRGDMLVLIDLLYLLKSEKTESIENKKIVVIRFNEVTL
                     GLLVDQLIDVIYLSKDQILSTPPDKNSLGENLMKGLCLYDTFTVGLVDTEKIFAYEKL
                     YVEEYIN"
     gene            complement(33492..34829)
                     /locus_tag="PLF66_08515"
     CDS             complement(33492..34829)
                     /locus_tag="PLF66_08515"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HMZ64121.1"
                     /translation="MNQFAIDISQIIQSRLGIHVTADKIDHYLDTLRKKSNLLKKISP
                     EKYIEYLKESSTLGNEEWKIIVDSLNISETYFLRDSGQFEILEQNILPAIIKEKQASR
                     KIRIWSAGCSTGEEPYSLAITLAEIPELMMGWDISILATDINGDSIRQAIQGDYLEWS
                     FRNVSNDKKDKYFTKKKNGYHINDSIKNKVQFIENNLFHSNYYNEFDLILCRNVFIYF
                     DDHSKKMILEKFEKALLNHGYLLIGHSEAAHLISNAFKIIPMQKSMVYQKKDHSPIPT
                     LNFSSKQGLSATIPTKPATTYTKIEKTFIDTKPILSIDHKETLYKARELADLGKVIEA
                     EKLVQELLNINPSNYEALFLQGQIIEAAGNWNQAIDIYKKVIYLEPNFLESYLTLSNL
                     YCLLHNIPESIKVRKAGLFCLEHDPRLRDVYIEKGYQIESLKAFFSEESSIWL"
     gene            35191..36318
                     /locus_tag="PLF66_08520"
     CDS             35191..36318
                     /locus_tag="PLF66_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000878132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HMZ64122.1"
                     /translation="MAVCILIGTVLSPIVFFGKKESGPIYLEAQSGTPVKDSPAKAQA
                     VALENAFQDVFDTVSQSVVSISTEKTVKIKNHPLAADPFFEHFFGGPKQGQELKQKQR
                     GLGSGIILNEEGYILTNEHVAGDMDKLTVKLKNKKTYEAKLIGSDKTIDLALLKIKAA
                     PGDLKPVVLGDSSKVKVGNWAIAIGAPHGLEHSFTVGVVSAIARSGIDASGLGYIQTD
                     AAINHGNSGGPLLNIHGEVIGINRMIVSPSGGSIGIGLTIPINDAKRVFEELKTNGKV
                     KHAWLGVGLETVNEEDIRELKLSDPKGAVVREVKKDSPADQAGIKIHDVIVKVGEKDV
                     ETREDVIEAVLNTRVGRKVEVKLIRKGSPMKVMVTTAERPF"
     gene            36330..37349
                     /gene="ruvB"
                     /locus_tag="PLF66_08525"
     CDS             36330..37349
                     /gene="ruvB"
                     /locus_tag="PLF66_08525"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020771302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HMZ64123.1"
                     /translation="MLANRILNPEDKSDDDNSLRPENLNDFIGQKEILENLHIFIQAA
                     KKRDSALDHVLLSGPPGLGKTTLAGIIAKEIGSNLVVTSAQVISKGADLARFLTMLNE
                     KDILFIDEIHSLNRNLEEILYPAMEDYKIDLVVGEGITAQTVQIPLKKFTLVGATTRN
                     GMISEPLRNRFGIQFRLDYYSDEEMAQIVSRSARILNINIEKEACLEIGKRSRKTPRI
                     SNHLLKRIRDFAEVKNDGRIDLEICKSSLQKLGIDHLGLDNMDRQILTCMIERYKGGP
                     VGLKAIAVVVGEEERTIEDSYESYLVRSGLVLRTPAGRVASELAYKHLNLDRKTKDEQ
                     RNLFF"
     gene            37324..38250
                     /locus_tag="PLF66_08530"
     CDS             37324..38250
                     /locus_tag="PLF66_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004474960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HMZ64124.1"
                     /translation="MNKEIFSFDLNSLDEDERRLLLSASLVFFFLSFTIAHLFTKNML
                     WRLLGTESDLVEMKEGKENQKVYEVLLEQEFVNKTKKDEIKALSNEDSAGTGGLTEKE
                     GFHTNSPFYEFIFGGLPSSSPQMVQKNNESKKTEDEIYEVGIYQNDPMMKQTPQTTSV
                     SNPATGQETKIPFNYRFQKDFEFRWDGSSQLSIPRKKMAGYEYFKHMIKQIEEAFAAP
                     GGGNFAYRDMAGTVIREGITPGETKVSFMLNDAGQVIDVKQMSSQGQEIVDRACIDSI
                     RGQNFGPVPPEVKEQGMIFGINFVFPGYVRYR"
     gene            38314..39282
                     /locus_tag="PLF66_08535"
     CDS             38314..39282
                     /locus_tag="PLF66_08535"
                     /inference="COORDINATES: protein motif:HMM:NF013989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HMZ64125.1"
                     /translation="MGKLIVFNFITLNGNYKDSIGDTSWHRHGEEESEYAMENMKSDN
                     ILLFGRVTYEMMAGYWASPAAKQNSPQMAKSMNDAEKYVFSKKLKKTTWKNSKILKGN
                     LEKEILALKQKSKKDIVILGSGSLAASCASLGLIDEYQIMIDPVLLDGAQIFKGISNK
                     IDLRLTHSRVFQKSGVVLLCYEVIPSRIQKIKIEKDINVFYVKAKSFPEGIHDAIDTL
                     QTLTPKSKKRRFFGISNPDKKGVIQYKACAEELKKQEGKRLGLETFRIKKGMYLSILV
                     KDFQKDPMQIGSAFQEMLFRPEIDPKGYCLEWYQNETDVLCLVPIG"
     gene            complement(39279..39713)
                     /locus_tag="PLF66_08540"
     CDS             complement(39279..39713)
                     /locus_tag="PLF66_08540"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: protein motif:HMM:NF025793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HMZ64126.1"
                     /translation="MIQTFLEKFISITRNHPEEIPSFSQIYLEGSLVSEAFNQVEENK
                     TTYKHSEILCIESAQKKLQTKFLNQSILLTTIEPCIMCAGAIIHARIEEVIYFLEATK
                     TAGITSLSFESIYSKNHFPKLTFLYSEDVEKIVQVFFKDKRN"
     gene            complement(39710..41848)
                     /locus_tag="PLF66_08545"
     CDS             complement(39710..41848)
                     /locus_tag="PLF66_08545"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HMZ64127.1"
                     /translation="MKNHRIIGIILFIILFFNSCDLSNLTGNKNKTLAKKGILDLREW
                     DFNKYGPIELDGEWEFYWEKLYSLEEIHSIIFNKEYIHLPHSWNNKIINGETIGSDGY
                     ATFRLKILLPPNSPALAIRSKQQATAFHIYVNGVSTLGSGVVGKNEKESIPHTVPAIG
                     YVPDLKMEDMEIVMHISNFHHRKGGVWHSLYLGNYYQISGLEKSARETDSFLAGLLLF
                     IFIYHLGFYQLRKNDKSTILFSFFFLLLFLRIITSGEKLITNYVLFQSYDVYLRFEYL
                     ILFWAPALAIHVLHSLFPKEIHRLLFLCYYGIGLLASFSLFLKTKHITYMFLILQPFI
                     LVALILIPYYLFLAVLNKREYALSIFVSTIAIMLTNIHDLFAANEIITTNLFLVPYGI
                     AIMAVTQSYALLLKFSSAFKRIEQLSGDLKRTNFAYARFVPYELILYLKKESILEVEL
                     GNQIQKEMTILFSDIRDFTTLSETMSPEENFNFLNAYLKRMNPCVQFHNGFIDKYIGD
                     GLMALYPVSPDDAIESAIEMQKAIRHYNDHRKVKNYKPIEVGIGIHTGTIMLGTIGTE
                     DRMEGTVISDAVNLTSRIEGLTKIYGASILMSIETLFSLTDADKYLYRILDRVKVKGK
                     SVNLTVVEILDGHSEEVFGLFKATKTQFEMGVSLYLTKDFHDAISCFKEISDINPHDN
                     AALLYLKRCTYYEKHGVPLDWEGVSSFEDK"
     gene            complement(41916..43097)
                     /locus_tag="PLF66_08550"
     CDS             complement(41916..43097)
                     /locus_tag="PLF66_08550"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HMZ64128.1"
                     /translation="MSRLILVLVGFLFVAAGVLTGVYVMVNHAEEKTVENNAYPFEER
                     LKIAEELLNQSSKKSNQKALLLFTELSSKSIPEKFQFRVRFGHAGALQKNKDRLRALD
                     IYKELNQMSNLTKEERESLSYALGNVLLLLNQEEEGKIHLNSVLQTSKDSQLRSKALL
                     SIADFYMRKGRYENASKNYFLAVQEYPNNVQARIGWTRALRKLGRDFASYDIFENYSE
                     EIANIDKEEVKTPPPAEDKQTSFDRAKTLYNKAQYDKAIKAFTRLLRITPAGKQKEKI
                     LYYIGESNLKLGRYPEAIKYAEEVLVNEPSSLDHYALYTKGMALFSSKKYDRAAAAFN
                     AIVDKYEPSPLTERAKKYSKECLQLLKEEANAPAKEEKKPADANEEPNVEEMDADDDT
                     P"
     gene            complement(43094..44692)
                     /locus_tag="PLF66_08555"
     CDS             complement(43094..44692)
                     /locus_tag="PLF66_08555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64129.1"
                     /translation="MKHSINKINPFLNLGWISILFLIIVFLFINPLSPQTEDTSSQDK
                     KKVERDIVSDLTATLSPDSISIELKWTPPQEEGDVVIARSNSVIDTVEKLGISDSLGK
                     YKSDKVNVFNSYKDINLRAGEYYYAIVLVSQVKKKRVKLYPGINYTVNPVIVPARPEN
                     PIPVSAIAEVKHEIDSISNLKVRKIDNTVRLTWTPPIDADKNSIKYTVYRSPDPMSNE
                     GLMEKATKLGETTHPETTFLDTTIDSTQTVYYGVTVTIGEKESIPLTEDKSFKKFYYI
                     KTDEPVKIVEEKKDPIQVPANKKPLSITKLSAKLRKDGILFTWIPQEGVVNNTTKYFL
                     YESSSKIANDTDSKLIEGAKKIGTIVHPDLTFLHPRLDRKGIFYYAVTLQNGSEEENL
                     VLKEDQSFIKLDLSKRKKKKKKSDEVKTEEAKVVEEKKTPTPQPQIVTDDSNPDFETI
                     MTQYYKKEKYTEALVKFKSLADRVSNNSLRGKSLFFAALCNYNLKDYSAALKILLSED
                     VQMNYDRERVDFYVKRCLENRGNK"
     gene            44841..45476
                     /locus_tag="PLF66_08560"
     CDS             44841..45476
                     /locus_tag="PLF66_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020769297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HMZ64130.1"
                     /translation="MISQEIDYYEDMKYQDFLMSSRRKVICPPEKIMYYLEIKDAMHV
                     ADFGMGKGFFIPYLKKKMNKEAHLWGIDYQQEILDLVLKRKVEENIQNFSVVHIDKTE
                     HPLLPNWIPLPDVVFSAMCLSTFPDPGLAMDGLIRSMKPEGRLFVIDWAKVEFAEGPA
                     IKDKISFDKMKYLAEFYKLKVVNAFTINEFVYGMEIVAGPEFKTQFYDYRE"
     gene            45481..45873
                     /locus_tag="PLF66_08565"
     CDS             45481..45873
                     /locus_tag="PLF66_08565"
                     /inference="COORDINATES: protein motif:HMM:NF024773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HMZ64131.1"
                     /translation="MKENKVLELLNKKDCTIAIVGATNDSSKYGNIIYKDLKGKNIKV
                     YGINPKATTIDGDKAYHTLDDLGFKPDIIDMVIPPKLGLALIQDAVSKGYDNFWLQPG
                     AESDEIIQFLEDKGKNYLAYSCVMVESR"
     gene            45873..47543
                     /locus_tag="PLF66_08570"
     CDS             45873..47543
                     /locus_tag="PLF66_08570"
                     /inference="COORDINATES: protein motif:HMM:NF015894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FapA family protein"
                     /protein_id="HMZ64132.1"
                     /translation="MNNNYTDSILKDLENKENGSFQIKNENNHAVLTVFKPGKNGRAV
                     TRQEVFQRLKLFSIEGYDRAQVEVIVANADGKDHPIAIWTGGNPVDSRVELEVEPDKM
                     SAYVIIYPALHGGKILKKADVIDILKANGIVFGLKEDVINHILDNNKFFQKTLVAEGL
                     KPTPSVNGYIKVLFEPHSKPNLHEDAKGRIDFKDIQVIKNTKQGDVLAEKVDPKIGTP
                     GKNIFSEEVTLDKPLEGEWKIGINCTLSDDKKKLHASIAGRPILERDGTIRVDEVCYL
                     ENVDYSTGNVDFPGTIIVDGTVADNFSLRTKGSLMVKKSVGRVFLYAERDIVLSGGVM
                     GRNGGVIESKADVYARFVEQGNIKAGKNIIIEEASLHSELVAGDSIVIKGGRGELIGG
                     EAIAGKSISVSKLGAVVETKTEVVVGLPPEILDELRKMKDEIASFEEVLKKVKQTMAR
                     LSDPKKELTQEEKQMLTKLYEVERKYNDLLVNSKTQYETLVVSYEPAPSAFVEVEKTL
                     FPKVSVNLGKGKIYHSELKIYEGKCYIYNGDDGIPMRSPVPPKKKDAE"
     gene            47546..47944
                     /locus_tag="PLF66_08575"
     CDS             47546..47944
                     /locus_tag="PLF66_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012390055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HMZ64133.1"
                     /translation="MCKTIKQKVKFKASPEVIYALLANSKKHSAFTGEKAMISNKIGG
                     NFSAYDGYITGVNVDLLPSKRIVQAWRGSDFPEGIFSMAAFVLVSTKEGGTELTLTHR
                     GVPKNLIPSIEKGWKEFYWDKMKDYLAQKK"
     gene            49241..49600
                     /locus_tag="PLF66_08580"
     CDS             49241..49600
                     /locus_tag="PLF66_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HMZ64134.1"
                     /translation="MWKIYRTDEFKDWFDSLEEISITAIVTDLLILEKYGPSLGRPYV
                     DTLKGSKISNLKELRTRSKSGVFRILFVLDPERDIILLLGGDKSNDKKFYQKMITKAE
                     KLYQEHLRNLGRIKNED"
     gene            49590..49913
                     /locus_tag="PLF66_08585"
     CDS             49590..49913
                     /locus_tag="PLF66_08585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HMZ64135.1"
                     /translation="MKINKKKDFISELRDSIPKDVFIKAEKKAQREIFLIELRELRKH
                     FGVTQQKFKNFTQSGISKLESRNDMKLSTLIDYMDDIGLGMEIKIYPKKNKKKEEKEF
                     VLLKT"
BASE COUNT        16622 a         7974 c         8686 g        16819 t
ORIGIN      
        1 atatgagaag caatttgcct aaaatcgtgt ccactaaata tgtagtagtt cagtttggag
       61 aagagagcac taaagtgctt agtgtggggt gaatttgggt tatggggaat gcttcagaca
      121 aattatggca ctttgggttt gaaaggtttg ttgatttaaa attattccag aacaggaatg
      181 gataagaaaa tgataccaga agaatttgag actaagagtg tattatctga tgatgatatg
      241 caaataattc taagtgcttt tagaatattg gaatttccga attataatat taatcctaga
      301 gatgtagatt atgcattttt ataccaagta gaaaaattag aaaggatatt ctatagagag
      361 ccaatagaac ttcagagaag gaaggatcat ttattggagc aatgcagaat ttgcgtatcg
      421 gcggcaaagc gcccaaagtt atttgaggag attattgaat ataagaagaa agagattaaa
      481 gcggatagtc atttcatacc ctttaagcac tataaagaat tttttttaaa cttatggtcg
      541 gatttaaaaa tttttcaacg ccgtctagtt gataaacctt taaaatttat aaatcacata
      601 ttagtatttc tgtggtcttt gggaaaaaat ctaattatcc tctcattaaa gaatggttgg
      661 gaatttctat attctttctt tacatcccct agatttttac attgggttaa tcaaaattgg
      721 aaaacgagat taataacaaa tctgtttgcc actatcattt cattcattat gcttttcata
      781 cctatataca ttattaagct tgcatacaat aatgttagag aattttggaa tgaccaactt
      841 acaaacgttg aaaatcttcc tagcgaagat ttaatcggtt atattcctaa aaattatata
      901 attgtaaaag ataagctagg tggtgaagaa ttattagcca ttccttttgg aacggaaatc
      961 aaatatagaa aagtgaatat tagtatggat gaatttgctt attatattcc tagcattgat
     1021 tcctatattt ttaatgagga actcgcaaac tctgtccctt ctatcgttgc ttctaaagtg
     1081 ttcactttaa tagaagaaga aaatcaatat attcctctgc aaaattatac aaagaaattg
     1141 cctgcaaaaa agattgaact cttttcaggt aactttagaa ttttcgattc tacaggcaaa
     1201 agagaatacg agggaacata ttcaattaat aagggttata tttctctttc ctctcaaagt
     1261 ttaaacggaa aattaaaata caatcatgat ggttcttttt atttctctac aacagtattt
     1321 aaataaacaa taaaggttta taggcaagta atcgttgcaa ccaatcaata tgagaaacta
     1381 ggttatatat ttggttcatt agagaatgtt tctataagag atcagatttg caatatacta
     1441 gaacgttgta aaactgcatt tataaaatat gaaaagaaat aaggatataa cgcatggaaa
     1501 attttgatcc gaatcaatta gacatgcgtt ggtatgcgac atcaaaagag gatcgtcttg
     1561 atttgctcta tgatttagta aaacaagtct ttcgaaaaat aacagaattg gaagatgaga
     1621 tgaatgaaca gcaacaaaaa gttttagatg caatatctaa cttgcaaact tcccaagacg
     1681 agaattttaa aaaattaaag gaaactattc caattcttga attattgaaa attagaacac
     1741 gcttacaaaa aaatatattg gctagtttaa attcaggttt taatttaaca ggaacaaatg
     1801 tagagcaagc acttgttgct ttaaatgaat tggatgaagc attgaagcaa tggacttgaa
     1861 tgttttcaat tcaggtaaaa aaatcgattc attgtataac aaaattgata tagtgattct
     1921 gaataccgaa ttacaataag cccgaatgga gatttcgctt cgattggaag cgggaataag
     1981 agaaaaatgt ttttagtatt aacatcaact ttttctattt ccaatccccc tcgatccccc
     2041 tttgcaaaag gggtgttcca gtatgagaat cttttcgtaa gaagacgtta gctatagagt
     2101 aacactaaat tgaaaagaaa taaaaaatct ccaaatccta aaaatcatcc ttctccttaa
     2161 acaaccgaaa tacaaaaagc ccaaatggag aatcctgcgt gttagtagta gaatgccgca
     2221 gtttcgggtt ttttctttgt ttgttacttt atttcttttt tatacaaaaa agaaataaag
     2281 taagaatatg ctgggaagcg ggaataagta aaaataaatc ttaaagatat taatagacca
     2341 gcgcgaattg gcgaattcgc aacaccgtta cttcacaatt ggttgcatgt attgaattgt
     2401 atcttgatct aagcaaacca tcgcttctgt ttttttatag gaattttttt ggcgatagaa
     2461 tttgagtgcg gggtctactt tcataaatcc taatacaaaa gaaaaggagc ttcggaaagg
     2521 aaaggttgtt gtcatacaaa gattttctgg aatggattta atttttactc catccaaatc
     2581 ttgaaccgtt tctaagacaa tgaaaccaac ttctgaaagt tttttttctt cttcggggta
     2641 gaaaatttga aacgatttaa cattggaaaa attatattgt tttaatagtt ctccaatttc
     2701 acgtgttgta tttcccacaa gagaaaagtc ttttccgttt atttctctat aaataaaatg
     2761 aaaaggtcca acgtttcttt cctcgatagg aacaggaaga aatgctccca tataaactag
     2821 atatcctaca attccaaata taacaagaat agggagaaat gccaaaaaga acatattact
     2881 tagttacctc aactgcttgc aattcagaag ttgtttgggg ttgtgtattt acaacctctt
     2941 gtgaggaagt gggaacattc ttttcaaaat gttttaattc atttttaaag tattcgatta
     3001 actttttatc caattgaaat tcaaacaaat gaaatgcaac tctaactaaa tacacgattc
     3061 caattccaaa tgcagcaggg agaatataag ctggcagtaa atagaataca gctgagagta
     3121 gaataatcgt tgctggaaca aagatcatag agagttcagt gattttattg atgatttcaa
     3181 catttctatt ttttagattg tttattgaat tctctgtatg ctctttaaac ttctcttcga
     3241 tagtagattg aattttaaaa atgtatttag attgtttatt gaaattttct tttagatttt
     3301 caatctgaga attaacgttt gagagatcgt tctgcactgt gtttagattt gtttttagcg
     3361 ctgcattttc attttgaaca ttttccagga ttgtagaaga ttctgctcgt aagttctttt
     3421 tgaaaaaatc attttgttta tttgtaatat ccgtgtagtg aagatttcga ttaaaagaat
     3481 cttcgaaaag taaagatgct attgacgctg ttgacgattt taatctttct tcaatggaat
     3541 acatcgttag cataatttcc atgaagtaaa tcatatttct ctctaagaat aaatccaaat
     3601 tatcagggtg atcgagtagt tttagaattg aatcataatt tttcatttct ccattgactg
     3661 attcatagaa gatacaagca attaaatgct tcttaaagaa agaggtagaa gttttaatta
     3721 aaaaattcgt tgtgagttca tgcacttcat ctttattttg caagaatcga tttatatagg
     3781 acttgtaaga ttgaagtaat tccttctctg ttttataagt tggatttgta agtcttatat
     3841 aaaaatgata aaccaatgga aggaataatt cattatagaa agaattccaa agaagtccac
     3901 tttcatccac tgctgtaatt tcttttaaga tgctttcaaa ttcaagtttt gtatttccat
     3961 cttcttgttc atcaatccca attacttttg tgtaatagtc ttcaacttct tgttgtttac
     4021 ttggattgat taaatacttt tccccttgag gtagaatcaa ttgatcttcc tttgaaagca
     4081 tggaaaggat ttcttcgaac tctggaacat tttgcacttt cagagaataa tttgtagata
     4141 aatacttaat caattcatcc gagccaaagg cacgtcctgg attatctaaa aatatagata
     4201 ggattacatt tttatacaaa ttaggaaacc aagcgtttcc atccaaattt aaaggatata
     4261 ataaaggtat cagagctgtg tattgattca ttctatttat attctcgctc tggaaagcag
     4321 attcctcaag caatttcaga atctgcacag cctagagaat gaaaatacta gcgtatgtat
     4381 tgtttagcga aaaaatccca ctctaaaggc ttacatttac catgatcatg tgctgggatg
     4441 ttatgatttt cccaagcatt tgcttttgct tcgtgttccg gaggaaaaat gacactagaa
     4501 tctccagaag gagttccatc atcattgcat ttctttgtcc atctgtcatg tcggagacat
     4561 tttcgttttg catctacaca attcatattt ggcattccct tttccccaaa aaaagaaggg
     4621 tgtttacccg gcggatactc actgtttgtt gctaatcgaa gtgagttggg agcctttgca
     4681 tcatttacaa tataaacagg accaaaggca ctatgtgtat cttggtattc ataaccctct
     4741 gactgaacac aatttttagg gtcatagcct ttgcctaaat ctgaatattt caccattgga
     4801 ggacaagggt ccacatgtct tacttctaat tcaaagggtc ttgctacttc tagaacgcaa
     4861 ttaaattcat aaccataccg atttgcatag ggaagacttg ctctatagtg aaaaaaggta
     4921 ttactgtgtt tgatcttaaa tctatgatca aacgcttcat ttgtcttaga atctactcca
     4981 tgtgtttcat agcgatcagc ccaattattc catcttgcat gccgtcctag tttatcttta
     5041 aaaagaccgt tacttgacat atgtggaact ggtggtatct tattgtagca gtagtcttct
     5101 gccatttcat atgctacatg taaatccgaa gaataaactc tacgaccagc aacatctttt
     5161 gtgtcataag gtctaacgca agtatcatac ggggttcttg gattaggagt ccaatcaggt
     5221 cttccatttg gagtatcttt cggtagaaat gctctaaagt cttttgaaag aggatcactt
     5281 ggattcggaa ttaagctttt tcgtatattg tcttctcccc ctccaacgct aggatcaaaa
     5341 gcaacatttc ctttgcagta tgagattaaa ttctttagac cttctggttg ttcactatca
     5401 ggatcaaagt ccttattgat aatataacgc cattgtagat agtttggtag aaatttaatc
     5461 ccactctgaa aaggtccact gttaaaatcg accgatttat ttagggagcg atttttattt
     5521 tgggtatttg gatacgttgt gcgaaaccat cttgcatcaa tcacagctct cacatatcca
     5581 ggcaatttcc cacgctgtcc gttctcatcg attggtaatg cgggaaatcc aaaggaaatt
     5641 ctagcaagcc cttgacagct ctctgttgca taggcacaac aacaattaaa accatcatag
     5701 ttcattgtaa aaattccatt taaacaattt ccaaaatgag cttcacccgc aagcattgaa
     5761 aggtcttctt cgaaaccttg gtttaaaaaa tctttttcta atctggctct aggagatgta
     5821 atattatctt catctacttg cccgtcattt tctggagaag caaagatacc tcttccgatg
     5881 attggaattc ttctatgagg ataaagtgga tcatgaaatt cttcttcggt aattaaatca
     5941 tccccgaagg aggttctggc tggaatttct gtaacgtaat caattccaga tggatgcctt
     6001 gctttgtttt cgcgatgatt cgaaaaaatg gaaaatgcat ttacccaaaa aatgaaaccg
     6061 gcaataaaga gccaacctaa gcctataaat ctattttgta ttttgtctct ttttaatatc
     6121 acttttaacc tttcctattt tacatttttg cgttatggat ggtgagatta gaaaaatcca
     6181 gcagatttga tgtggattct caccatgcga attcaagtta cctttatttt taaaaatcgg
     6241 ttacaatata aatttttatg acttagattt ctactataaa agctacaaga aatttggaaa
     6301 aagtgtagta atataaaatt ctagaaggat tgattcggga aaaataagca ccattagcgg
     6361 ttttaatttg aatggattgc aagctagtag ggcgttttca atcgaaacaa gaatttccga
     6421 gtgcctttct tatttttaaa caagggattt ttgggagttt acacttttat ttggttttga
     6481 aatcattcta gacctgagtt tttaaagaat ttggaaactt aaggataatt agtcttcaca
     6541 taatattcta gaattctaat aaactcataa aatgtcttgg gttcaaattt gattcaattt
     6601 acaaagaggc taatatcgat ttaactctaa attgaatttc agttgctgca ataaaaaata
     6661 gaatgcattt ccactcgtgt aagaaatgat ctttgttatt tcttctctca ggaaaggtat
     6721 atccaaatca aaaacagaaa cccaatttat ctcagttcct tcttctgttt ctttgaaatt
     6781 aactctacca agtaaatgaa caagtggtat atctattctg atcaagccaa ggttcacata
     6841 acactgaatc actttatatt caaaaaaatc attcctttta aaagctagaa tttcctctag
     6901 gattgttcct cccaaactat ccaactggcg aattgcaccc ttcccatttt tttcatactc
     6961 cccccattga attaacttag aagagagaat tccattgaat ttagaatatt tttcatgatc
     7021 agaaagaaca tcgaaaactt tttctattag agccttaata tcaattttta tatctatctt
     7081 ttccatagtt ctttctcctt atttgttgcg gttaatagag atttaactca atctatcagt
     7141 tcatttctga ttgaaaaaaa tccaaactaa attaatttag aaaagggtct catttaacaa
     7201 tatgtatgcc actaatagtt aaaacgattc taatttattt atattataat attaataaag
     7261 tttcttctaa aaagcgaaaa tatttttttc aaaaagcaac tgaatttgaa aaatatttct
     7321 tgtataactg ttaaaaataa gaaagattca ataaagcaca atgtaaaaaa aacaaatgaa
     7381 aagagagtaa attatgagtt cgattggtat aaatcctaaa actagttctg aatacaaggt
     7441 ggtaattgca gattcatcta atattgaacg aaaactctta gcaagatttc taactttaaa
     7501 ttcattcaag ataacaaatg aagcagatag cacagaatct attcttcata atctagagtc
     7561 tcttgatcca aaacctgata ttatctttat agatacaacg attcttggta aagaaggagg
     7621 acagttttta aaaatattaa atagtaattt tcctgaaatt aaagtaatat ttattatttc
     7681 ttcaaaagtg caagaggcgc agatgttgaa aaataaaatt aattactaca tcatgaagcc
     7741 ttttctaaaa aagaatatcg aagaaaagtt atacgaaata ctctctgaca aaccaatgga
     7801 aatcaactcc gaagataagg gagatgtgca tctcaagaag gtttttattc ctccaattcc
     7861 gaatgcagtt cacaaaatat tgctctttga agcaaataca acaagtggta gtaatgagtt
     7921 agaagaatta ttatacccag ataaatctct atgtgctgat ttattacgtg tagcaaactc
     7981 aagtttatat ggtagaaatg gaagtataaa agcattacat gatgccatta ctttgcttgg
     8041 tataaaaaca attaaaaata tagtgattgt tcaggcaaga agacacatga caggaaatct
     8101 tttaaaagaa tctattttta aaaagttttt atatgagttt tctattttca attctctcat
     8161 atcgcatgat ctaatttccc tttttggtaa ggcaagagag tataaagaaa tatttctact
     8221 atcgtccttt cgaaaaatgg gcatgaatat acttgctctt aacttccctg ataaatatgc
     8281 aaaaatctta aaagcatttg agaagggaga aggtgatttg attgctttag aaaaatcaga
     8341 actctcattg gatcatgttc aggtgggtgt atccgtgatg gaagagtgga aaatgcctat
     8401 tatttttaca gagactgttc gagatcaaac ctttaagcct tccaatttca aagatgtagg
     8461 agatattgat agagttacta gaatggcgga ggtcttaacg aagcatttaa tgaagattac
     8521 tctctccgaa ctagacaatc aaattttaga agaggcagat catttttata accctaaaga
     8581 tcattttatt gctctttttg gtgaggatta tcttgcgaat ataacgtctc atccattttt
     8641 tgaaattcct atctaaacat tataaaaatc taactgattc aaatattcat ttttaattca
     8701 taactgacat taggcaaaag ataaaaattt ataggataaa taacaaaaat tattctatta
     8761 cgtttcttta atgaatcaat aaataagtgc gttagggcgg ttagcccaag ttaccgacag
     8821 gcatgccgtc aacttaagag aaatgaaaaa gaattaaccg cgaagagcac agagaacacg
     8881 aagaaaagag aaggtttatt atttattcac ttcgtgagtc ttcgtgcatt cccgcccatc
     8941 gcttgacgct caccctagcc aagctatgtg gtttaaaatc cttaagttga cggcattgac
     9001 cgacaggctc actgtctctc attttacgcg ttcgtctgta agatgattta ataatatttt
     9061 agaatttaac aattttaaat tcgattggaa tttaaattag aaactgtata caatcattgg
     9121 accgatttcc caagctttca tgatcattgg atttacttct ttttgagcat atacgccgcc
     9181 tatcgttatc ccaaagtatt tagaaaacat aaatgttaag agtccacgaa tttcccatgc
     9241 agctagaaca tagtttctgc gaatatctcc tcgaagaata ataaacggca ccggcttata
     9301 aagtattttt aaaccaacat taccaaagtt taatacttta ggactataaa gattaaaatt
     9361 gtaagcagtt ccaagatttt ggtaagtcca agcttttaga atatcatctg gatttgtagg
     9421 aataaatccg tttcctactg tagttgctcc caaagagggg aaaacattta gataataatt
     9481 tacagatcta tattgaaaat aaggttctat ttctagagtc tctgtaatcg ggattttagg
     9541 tcccaatgaa aacatattaa ttctcatttt atcatttact tcaactcttg gaaaagcaat
     9601 aaattcagtt ctatattcag ggctatcaat taccttctcc caggtatagg tcccacttcg
     9661 aacaaattgt ctgcccgaaa aattaatcgc atatttagat gtaataaacc aattgtagtc
     9721 aattctggaa ttatatatat tttgcaaatt tttaccatta aaatagatta gcggtaaatc
     9781 attattaccc tctggtgaaa gattcattag catctttgcg tatgtataac taaaaagcca
     9841 atttttattt gttaatccaa tttgagtcga tgcatttgat cctgaaaatt cagaattcat
     9901 aggagttaat ccactattag tcccaattct tttgctaata gagggattat taaaacgata
     9961 tgtagtttga tctttaaata cgaatgggac cgttggatag aattctccag ttgtgcacct
    10021 gcataataaa ttaaacgcca tttatcttga tcagttgaat tatttccact ggcttcggga
    10081 tagatttgaa ttgcatttat tataagaaga aataagaata gatacttttt cattcgatta
    10141 cttagaatat tattttctcc aatatctaac gcattgtttt tgttacaagt tcaaaaagag
    10201 tagtttacct tttgcctttt tctaagtttt ttaatgaatg atttacaatt ctttttagtg
    10261 actcacatta cgcaaaattt atcctttgag aaataaatga tgatttttaa gatattagag
    10321 aatgctaatg aattaataaa atcaatagct gtgatagctc ggctgctgga gtgtttataa
    10381 agcgttagtt tcttatgaga aatgttccta ttttacgaat agggtttcat tgaatctaag
    10441 attaagatct agataaattt agtgattttt tacttattgg aaacttgaaa gaatttattt
    10501 tgataaaggt atttctaaaa tatttttact tcgattcttt tgatcaataa aaacagatcc
    10561 attaatttga tgggcaagca tatggatcag ttgtaatcca aaaccagtag aattttcata
    10621 gctaatacct tctggtaaac cactcccatt gtcttcatag gtgatcctta tgtgattatc
    10681 tagtctttct gcacttactc gaatagatat ttgaccctta gtcgaattgt atttgatagt
    10741 attagtaatc cattcattaa aaattatacc aagaggagat aggattgtag gtgtgaggat
    10801 aatatcatcg atttttgttt caatctgaac aggatttatt acataaaaaa tatttactat
    10861 ttcctgaata aaacttggaa ggtatgtctt taacgatata tttccattat cctcagtatt
    10921 gtataatttg tcatatagga tcatcatact ttttactcta cctgccgcat cgagtaatat
    10981 attcttaatt gtctgatttt ccagtcgact ggattgaatt gttaagagtc ctaaaattgt
    11041 tagcatatta ttttttattc tatggtgaac ttcttttaag ataatctctt tttcatttaa
    11101 tagatttatg attttctgct ctgaattttt acgaactgtg atatctctta atatagccgt
    11161 tattattttc tcttccccaa gatttatttt tgatattgaa acttcgataa aaaactcttc
    11221 accatttgat ctaagtccaa ccatttctat gacttgtccc tttttgcgct taacttcccc
    11281 taatcttaaa aaaatttcga cactatcagg atatttctct ttgtatcttt gaggtattaa
    11341 cacatctaga tttttgttca agacttcttc ggatttataa ccaaacattt tttcggcagc
    11401 agtattaaat agtaaaactt tagaatcttg atttagactt atgattgcat ccattgctga
    11461 ttctacaatt ccatttaaac gagcttcgct agctttttgt ttttcctcca attttattct
    11521 gtttgttaca tctctattca cagatatata gccaataacg ttaagagact catctcgtaa
    11581 aaggacaata ttaccctcca tataaaactg agttccatcc tttctgcttt gaataaatac
    11641 atcgaagaat tttcccttat ctaaaacact cttaaccaca tcgcttcttt cttttcctat
    11701 gtagattgtt tttaataggc tgggtgcata ctgaccaaga gcttcctctt ctttccatcc
    11761 ataaattttt actgctgaag cattccaata tgtgattcgg aattcagaat ctgttgcgat
    11821 tatagcgtca tttacatttg cgagcatatt agcctgattc gagattaatt tctcatattt
    11881 ctttcgatcc gttatatcgt aaatagtagt aattgcataa tcattatttt ttaattgaac
    11941 tttggaaaca tgtatagtta caaactttga ttcacctgac ttagtcgtaa tttctctctc
    12001 tcctgtgtaa cttttttggg gactattgac atgatactct gtttctacct gatcaatttt
    12061 ttttacaatt cccatttcga ctgacgtttt ccctacgact tcattcttgg tataaccaaa
    12121 tatattttca aaagattcat ttacatcaac gaattggttt tctggtaatt ttgtaagcac
    12181 agttggaatt ccagatttat taaaaagtat ggagtatttt ttctcgcttt cttcaatttg
    12241 tgttaacatt gcatattctt ttgtaacgtc tctgaaaaca agaacaacac ctataatgct
    12301 accattctga tttctgatag gagagcctga gtctgctatt tgaaactctt tacctgtttt
    12361 agaaaccaag attgtatgat tggcaagtcc aacaattttt ccagtggaaa gaactacatt
    12421 gactggactt tggacaggtt ctcttgaatg tgcgttgata attttaaaga tatcatctaa
    12481 gtgtttaccg tttgcttccg aaaagagcca acctgtcaac tgttctgcaa ttggattcat
    12541 acttgtgatg ttgccatgtt tatcagttga aattactgca tctccgatag agtaaaggat
    12601 tgcgcctatc tgttcttttt gtctttgagt cgttttgttt gcctcatata atttaaatgc
    12661 catttttatt gaagcatcta aaacggttat cccagaattc tttacaacat aaccataaga
    12721 agagattttt tctgtcttct ggactatctc cttttctaca cgagaggaga ggaataaaat
    12781 aggaatatca tgttccttta aaatctctgt agctgtttga gttccatcta atcccacacc
    12841 tagatcaata tccattaaaa ttaaatcgat agactgtcct ttctcattaa tagtggataa
    12901 tgcctgttct ccgctactga catgtaaaac ttcataacct tcgttctcta attgttgctt
    12961 ttccataaat gcgataagca attcatcttc aactaatagt atggtttttt tattgattgt
    13021 gttcatagaa agtaaaaccc catagtatat ttaccgactc atctgattct ttaggtgtta
    13081 aaaatacttt ataaaagaaa tgatatagtt taaataagaa aagcgttaag catgcattat
    13141 tcgatatcaa tcgagcaaaa aagcgttctt ttgattttcg taattcattc gtaatcccga
    13201 ataccaagta aatcatcttt tattcctgaa cttatcaaac ttattttata agatactata
    13261 taaatagttg aaatgaattt caggaattgc aagtgaataa tagaaatcct tctaaaaaaa
    13321 ataaataagg gaaaactctt gcaaattatt ttttaattat agttatttgg atagggtctt
    13381 ttgcggataa tacgaatata tcactgattt aattagatac ctagcttttt aaagcgatac
    13441 tagcagatga tcagagattt ttaagatttc atcttgcatg gaccttgcct caaatttgag
    13501 gatttagttt aagagagcca aacattctag ttgttgtcag cggacttttt gtaggctctc
    13561 tttcgactgg gttctattat aggcgctgga atatcttaag agttagagac ttataagtct
    13621 ttcgaatatt gggctcattt tgcctccgag ttcagaacag tctggtgact tgacaaattc
    13681 gacctaatat gtaacttttt tttagggatt tatttttcct tttatatttc taaaagtcta
    13741 acgattgata gttttgaatc aatttgcaat atttctgaaa tgagtcaaat actccatttt
    13801 atattcaatc taatcaaaaa ttcaatccaa tagaacgata gtttttagat agaatttttt
    13861 atgaaaaata ttcttttaaa ttgacataga actgaaaatt tctttttatt tcctttaaaa
    13921 ttttaactaa cgagggttta tgaatttttt caaatactta caaaagagca ttaaaaatct
    13981 gataaatact ttattcattt ttgtagtcgg tttattaact gtttttggac tccttttgat
    14041 tcgttcttat gtaatgagtt atttgtctag ttatagaatt ttagaaaacg ccagaaatgt
    14101 aggtcagttg aatttaggaa caattgagct atctttagaa agaagcgtat ctcaggtcac
    14161 gctcaatata ccctctccta tcactgtgaa ttttcaatct ttgatcagaa aacaaagaga
    14221 tctaggaact ccaaaaattg aatcagttat aaaacattta tctgaatcag gaaataatcc
    14281 aaaggcagca gatcggttaa aatcctccct atcagagctt aacgatctaa gaaagaccgt
    14341 agataaaaat ttaatgcttc cgatagaaga aagagaaaaa gaatttatac agaaattcca
    14401 tattcttttt ccaaatgtta tagaagagat tcaatccaat aaatttgctc ttcaatcaga
    14461 ggattcagtt cttccaacaa aagttcagct attagaagca attacacagt atgcatggga
    14521 agtaagagaa tttggaggaa gagagagaac ttattttgct atcgctttgt ttaacaagca
    14581 gccgatttcg agtgagttgt tagagagaat gacaatgtta agtaatcgtg cggagtatgc
    14641 ttggaaaaac attcaaatca tgaaacaaca tccagacctg aatcaagagg tcttaaatga
    14701 gatagtccat ttagaaagag catatttttc agactattac ttgattcgtg aaaggttaaa
    14761 acaagaagca atcaaaggaa attatactat ggagtttgga gacttttttg aaaagtcttc
    14821 tgctgcatta ggaaatgctg aatcgttagc gaaactaagc gtggatacag ttattcttca
    14881 tgctgaaaaa gaaaaacaaa aagcagttgt atatctcata acgatttctt ttttcacgat
    14941 atttgcattg gttttgtgca tatatcttct gcattactta aatcaaaaag ttgtaggtaa
    15001 catcattcgt gtaacaaata ttttagattc tttatcaaag ggaaataaag acataaatta
    15061 tgagggcttt catgatttag aaaatgaaat aggctcgatg gttgagtcct ctaaggtttt
    15121 ccgtgaaaac ttacttcgcc tagaaaaatt gatggtagag caatcatctg ctgtgaacga
    15181 aactacacaa gttgtagaaa tgctagataa atcttctaaa atgtccgctg agcaagcagg
    15241 ccaagtttcc gagattagtc aattggtgct caaagcggcg aatgagggag agcagatggc
    15301 aagtcagatg aaaagtctgc aagctgagat tcaagataaa gcaattacga ttaatgattg
    15361 tataaaagaa ttagaaattc agacaaacca gattggaagt atcactgatg ttgtgagtga
    15421 attagccaaa caaacaaata tgctcgcctt aaatgcggca gtggaagctt ctcgagctgg
    15481 agaatttgga agagggtttt cggttgtttc catcgaaatt agaaagcttt cggatgagag
    15541 taagaattct gcacatagga ttcaaggcct aattgatgaa atcaaaaaat ctgtaaggaa
    15601 gactgtctcg ttgacagaga atggtaagca ggctgcgatc acaggtagta aatatgcaga
    15661 agatacctct ttaaaattta aagatgttgc ttcctcaatt ggaaaattaa gcgataatat
    15721 gagctcaatt gcaattaatt tacgccaaca agtcagagca catgaagaag ttttagtagc
    15781 catgaattcg ttgaataaca aaacaaggca attggttcaa atctaaattt tgttttaaag
    15841 gctcatttaa aacaatcatt gtttttctct agtctgagtt ttgatgaaag aagaaaaaaa
    15901 tttgagagga taataagcta ttatcctctc tgtcggattt catttgaaaa ttagaaaatc
    15961 aagcttctaa tcgtatcgat acgcagacat caatttagaa tcaacttcta tcctttctca
    16021 ttttagagag cacaaagatt tacacaacct tatgtgttga tattttttat aactaattta
    16081 gtggtctctt ctaacattgc ttctttcgca tctgagaaat ctgctatttg cgtaacgact
    16141 ttttcaactg gaaaatttcc ttgttctatt ttctctaaaa cttttggaat ccattcaaat
    16201 gaatttactc taccaattgt aaattcaact cccttatcat atatttctaa aaatggaatt
    16261 ggaaaagaat ttgtaaaaaa tacagaagca ctcgtaactt ttccatagga tcgaaccgat
    16321 ttaatggctg aatagaaacc ttctttcact ccgcttgcat ctgcgactag atcaaatttt
    16381 ccaagagagc ctgaatattc gtttatttcg agtgttttga ttccagcgtt atttgcaata
    16441 gagagtcttt cttgagagaa atcgctatag gtgacatctt tacccattgc cttgccaagc
    16501 aaagctgaat ataagccaat actaggagca tatccacctt gcactaatat agaatttata
    16561 tctgaatttg cagtatgaat tccaaccatt ttccatgctt caacaatgtt atcgctaata
    16621 cttgcaatag aaattggatt gatttccttt ttaattggca tgagcatatg agaagcatag
    16681 ggaattaata attgttccgc caccgctcct cccatttttt tagcggtaga tcccatacca
    16741 aagtcagaag gatgtgaatg cattgtatcg caggattttg aatcagaagc taggcaactt
    16801 tcacatttac cacaggagat ttgaaatgga ataatgactc tgtccccttt tttaaatttc
    16861 tcctttagat tttctgatac ttctaagatc tctcccacaa attcatgtcc aaccggaaag
    16921 ggtggtctaa aaaaagtctg tcctctaaaa atggcaagat ctaaatcgca tcgtgcgata
    16981 gtaattggtt ttacaattgc ttgatcttgt tctgtaattt ttggttcttc tacttcttcc
    17041 cactccaata catttcgttt tcgaaaaatt agtcttttca tagattatcc tttactggat
    17101 agaccagtta gtctatatat ttgctttaag tagactaaat agtctactct ttttttaaaa
    17161 aaagattcga tttttcagag atatgtttca gtgtagagga aatttatgga aaatccgaaa
    17221 gaacgtatta ttaatacctc tataaaatta ttttactccc agggatatga tttcacatct
    17281 atcaatcaaa ttattgatga gtcagggaca cacaaagcta gtttttacag atatttttca
    17341 gataagtctg atattggttt ggaatatctc tatttaaagg gagaagagat aagaacactc
    17401 attttcaagt taacggaaaa atcatcatcg attgaagagt ttgtaaaatc ttgggctgct
    17461 cttttaaaga aacaatcaaa gagtggttcc tattttggct gtccattggt gcggtttatc
    17521 tcgggtagtt ccattcaaga tgaaaagtta agagagaaaa ttagagaaac tttaaaatct
    17581 tggatgcttg agatcgaatt gttttgcgaa aaacttaaaa atagaaaaga gttaaaagct
    17641 aattttcaat ctaaacaatt ttctaaatta gttgttaaac tctttcaagg aaattcatct
    17701 gtatttgtga ttacaaatga tttgtctact tgggatgatt ttaaaaatga agtaacagca
    17761 tcaattcata atttttagag gatgtgagat aaaactttca atagattatt ttaactactt
    17821 ccaaaatttc atcgaaataa taaatcattc gactgatttc tttcatccaa ataaagaatc
    17881 agattttttt ttaaaataaa ttaaatgctc ctcttatatt atcctctttt gacaaaggca
    17941 aggattgagt attgctctac tgcgaaggaa aaatagaaat gcgctttggc taaacaaaag
    18001 agagttttct cgtccctgat catttcattt agtattggat catggcatag gtatttaatt
    18061 ttttttgttg aaaaaaatat ccaaatctat atagttgtat atacaactat taaaggagtt
    18121 tatctatgat tgctacaatg gaaaaaataa atgagaaaga aaagaggcct agatcggaaa
    18181 agttggttcg ttttcaagat tgtgatccac ttggtcattt aaataatgca aagtatattc
    18241 attattttat gaatgcaaga gaggatcata tggttgaata ctatggagaa aatctttatg
    18301 agattggtgg aaaatcgaat ggaggttggg tagttgcaaa aaatgaaatc agctatttat
    18361 atcctgcgat tgcaaatgaa cgtttaatca ttgaaactgc attgatttct ttaggcaaga
    18421 aaaacatggt aaatgaagct gtcatttatg acaaagatag aactcatatt aaagtagttt
    18481 catgggtgca tttcaccttt gtaaataagc ttgggcgacc aatatctcac tcagaagaat
    18541 tgcaaaattt tttagagtcg atactcgatc gagatgcgtt gttcggcata gaagactttg
    18601 acactaggat agcaactctt agaaagaagg ttaaagaaga aaagcgaaat agatatgcca
    18661 tctaaaccaa atgaaaaaaa aattaataat caggtaagaa caatcgctaa agaatgttta
    18721 ttgatgcgaa ctagaacttt gagtagagcc atcacatcta tttatgagaa aaaattgctt
    18781 aaacatggat tgaaagcttc gcagttaaat attttagtaa ctgttgcaaa ttttagttct
    18841 attcaacaaa agcgaattgg aaagtttttt catatggaga aatcttctct ctctaggact
    18901 cttgtccctt tagaaaaaaa tggatggatt cggactgaga aaattggtgt aagtcaaaag
    18961 gtttttataa ctccaaatgg tttgaaactt ttagaaaaag tttttataga ttggcaacaa
    19021 gcacaaaaag aagttcagtt attaattgga ccatctatgg cgatggaaat gaaagataag
    19081 tttaaattat taaacagtat cagttaaaca aagagagaag ttgtggatgt ctccaaatcc
    19141 tcgactcctc ctttggtttt ttccaataag agcaggtttc attctataca tcaacacagg
    19201 taggatatgt cttaggtgag ttttttcatt tagcgtgttc tttcataaac aaagtttacg
    19261 aattgataca tgtcagaaat cttatgattt acaattttaa acgatgctca aaaagttttt
    19321 tttaagtgaa tgttttttct agggaattag tattaggagg cttatatgga aaaaccaaaa
    19381 atatttaaaa ccagttttgc gagtgtatat ccactttatg ttcagaaggc agaaagaaaa
    19441 gggcgaacaa aagaagaagt aaatacaatt atctattggc tgacaggata caatcagaag
    19501 agtcttgaga agcagatgaa aaaaggtgta aactttgaac aattttttga agaagctcct
    19561 catttaaatg aaaatgtctc tttaatcaaa ggtatgattt gtggctacag agtagaggaa
    19621 attgaagata agcttatgca gaagattcgt tatttagata aactaatcga tgagttggca
    19681 aagggaaaga gcatggagaa aatcttgaga gcacctcaga aataaaacct aaagatcaga
    19741 atccaaattg atcctttatt acttttcatt gacttgcaaa tgattgttag attgtaagcg
    19801 acttttttta taaaaccatc tccaattgaa ttttattaac tttaataatc ctaaccaaga
    19861 gaatattttg aatcaaatag ctccactcag taaaaaagaa aattgggtat tttttaataa
    19921 aaaatcgcag atttctagga ttcaataggc gtccaattct gtcaaaaatc tttatgtctc
    19981 tattttaaag ccctgcggat aatagtgatt agagtactaa acatacgtta tgcgtaaatt
    20041 atgtttagta caatcaataa aaataagctt ttttttattt ttttttcgaa aaaatccttc
    20101 agtaattttt taaacgtacc gtttatttta aggaaatcta tctgacataa ataaattaag
    20161 caggcacaat cttggaaaag aaacaatcta aatcacaatc attaaatgta gttccgacag
    20221 aaaataaaaa tgaacaacct tctacaggac catcaccgaa ctttaagaag atcttatcca
    20281 gatatgtaaa ttatagagga atagacatcg ttttgtatct caaagatgga acagttgtcg
    20341 agttggataa aaacagaaaa ctcgatgggg atgtagtgat taaaaacgga aaagagggca
    20401 tcgttgctcg aattgaaatt tcggacatac gcaaagctga tttttttgct gcttaattcg
    20461 attgtcttga ttgaatgttc caaaataatt cttgctatcc gtcctacttt ttaaaatctt
    20521 gttatattgg gctttctatc tgattttatg aaagtcaaca aggagtttag atggtaggaa
    20581 tcgttattaa agaaggagag tctattgaag gagctctcag aagatttaaa agagattgcg
    20641 caaatgctgg tatcttaagc gagatcaaaa aaagagaaca ttttgaaaaa cctagcgtaa
    20701 tcagaaaaaa agctattgat actgctaaaa gaaagcgtga taaaaagaaa cgcattttcg
    20761 ctaaaaaaga aaaaatctaa ttcatatttt ctctaaaaca gagaatattt tgattcactc
    20821 ttataaattc aaaaacatct ggtataaata tgtctttaca agttcgcatt aacgaagatt
    20881 taaaatcagc actcaaatca aaagaagagc caaagctgtc cactcttcgt ttactcaagt
    20941 cagatataca atatgaatta acgaagacag gtgcttcaac cctcagtgat gaccaagtaa
    21001 ttgctgttat ccgtgccaat tcaaaaaaaa gaagagaaac agcagccgaa tatagaaagg
    21061 ctaatcgcga agatttagca ttaaaagaag aatcagagga tgcagtcctt gtatcctatc
    21121 ttcctgctag catgccagaa gcagagattc gtgaagtcat tgcaaaggtt atacaggagt
    21181 tatctcctaa gccatcggaa gttggcaaag taattggaaa agtaatgcaa gaagtgaaag
    21241 gtaaaaatgc ggatgggtct ctggtttcta atcttgttaa atccatgatg gctgaggcat
    21301 cttaattttt tctgtgcaaa ctggcaccga ctttgtatcc caagtcaaac aacaaatcaa
    21361 cattgacgct tatatctctc gctttgttaa attaaaaaag caaggtaagc gtttcagtgg
    21421 gctatgccct tttcatggag aaaaaactcc ttccttttat gtatctcctg atctgggagt
    21481 gtatcactgt tttggctgcg gaaagtcagg ggatttattt aagtttgtta tggactttga
    21541 acgagttgat ttcaatcgtg caaaggaaat tttatctgaa tattcaggca ttcccttaaa
    21601 agtttctggg catagcgatt taaatgaaaa aaacgctatg tatgctttga atcaaaaatt
    21661 tttggaattc tacattcaaa atctaaaatc agaccaaggc agacaggcta gggagtattt
    21721 aaaagctcgt aaaatcagtg aatcggaaat agaatatttt aaattggggt atagtcttcc
    21781 gggctttgac aattggacaa aattatctct ttctcagccg gaaatcaaaa tcgcattaaa
    21841 gcttggactt ttaaaggaaa atcagaatac tccaaatcgc ttttatgatt tctatcgaga
    21901 tcggattatg tttcccattt ttgaaacaag tggaaaggta tgtggctttg gaggacgtag
    21961 gattcaagac agtgaagaag ccaaatatat aaatagtccc gcctctctga tttatgacaa
    22021 aggtaagatg ttttataatc tctctcatgc tcaaaactcc attcgaaaaa caagaactgc
    22081 catattggtg gaaggatatt tagatgtatt gggacttttt tctagagagt ttgacaatgt
    22141 ggtcgcaccc ttaggaactg ccttaacaga caaacaggtg agaaccataa agaattatgc
    22201 ggataaagtt ctcatactat ttgacgggga caatgcgggt aaaaaagcag cccttagggc
    22261 aaccgaaatt tgcattcggg aaaatttacc cacagagatt ttactcctag aaaatgggaa
    22321 agacccattt gatctttctc gagaaaagac aagggtagaa atcctagagg tcttttcaaa
    22381 aacaatcaca gcatctgatt ttgtgatcca agaaaccatt caaaatacaa actctcagtc
    22441 taaaccagag caaaaaaaga aagcggttga aaacttattc caatttatca aaaccttgga
    22501 aaaagaaacg gacaagcaat cctatttggc tgaaggagcc aagcatttgg gtttaagttt
    22561 ttctgctcta ttaaatgatt ttaaaaaaga atcaatccct aattttgagc ctcagaaaga
    22621 cgataataaa tataaacctg ttgctaaaaa agacttatcg ccagcagtaa agtgtgagag
    22681 aaaactgatt tccatgttaa ttttaaatga ttctcttttg catcatataa aggatttgca
    22741 agacttagaa ttcttggatt cagaaagttc tattctaaga gatttgatat acaatcgata
    22801 tctagacaat caagagatca gctatgaatc tatttttcaa tcagaagaaa tagatgagga
    22861 aattttgagt gtaattactc cttacatgat ggaagaagtt gagtccaaag agttatcaga
    22921 agaagaaaaa gaattaattt ttaaagaagc attgttgcaa caaagaaagt ttgtcatcga
    22981 aacagagatt aataagctct cactcagtca aaattatata gaggatgcca aaacaaattt
    23041 ggaaaagctg atgattctaa gaaaagagaa gcaagcaatt gttgaatcaa ttggctcgct
    23101 cagtttagcc aagaaagagg taaattaaaa atggaaaatt tacaaagtct cccagaagta
    23161 caaagaatta tcgcaattgg aaaagccaat agtgaagttt cttatgatga aattaacgag
    23221 atacttcccg ataaaatttt aaactccgag aaaatagacg atgtatttac tctcttacat
    23281 gaattaggta tcgaagttgt agaggagtat agtaaaaaag cggttgagca acctatcgtt
    23341 cccccaaagg aagaaaagcc aactaagaaa cggaaggaaa gttcctccat ttctgcaact
    23401 tcagaagatc caattcgttt gtatttaaaa gaaattggaa aggtaagctt aatatccggc
    23461 gaaacagaag tattcttagc taagcggatt gagaaagggg aaaaaataat cgaagagact
    23521 attttatctt ctagcatttt aagagcaaac tttgctaagt taatgccaaa gatcaaaagt
    23581 aaaaaaatca aagtttacga acttgtaaaa gtcgataagc tctatgcact caaccaaaac
    23641 gaggcagaca aattagaaaa agtatttttt tctaatatgg atatcattca gaatgaagag
    23701 aaggtctaca acgagtctgt caatcgtatc aagaaatatt cagaaaatag taaaaaatat
    23761 aaagagttgc gagagaagat tgagattaca tcttccaaaa ttgacaatgc aactagaaca
    23821 attggtgttt cacaaagaga gattcaaaaa atttctcaaa agatcaagtc gatggtattc
    23881 cgtatcaaag aaattgatcg ccacttttta aaaatcaaag ctcgttatgg gcatgatgta
    23941 aaagaaatta aaacatttaa ccgcttcatt gaaaaaaatg aaaacatcga agaaatcgaa
    24001 aaaatgatgg gtatcgatat tgatgaagtg agagaagtaa tcaaagatat tcgaaacaat
    24061 gaacgtaagc ttcgtagaat ggaacaagaa gctggttctt ccactgcaga aatcaaagag
    24121 tggggcgaga agattatgaa aggcgagaga gagatttctc aagccaagaa agaattagta
    24181 aaagcaaact tacgtttagt tgtatctata gcgaaacgtt atgcgaaccg tggaatgcac
    24241 ttctttgact taattcaaga aggtaatatt ggtttgatta aagcagtaga taaattcgaa
    24301 tataagaaag gttataaatt ttctacttac gctacttggt ggattcgtca agcaatcact
    24361 agagcaatct ctgatcaagc gagaacgatt cgtgttccag ttcatatgat cgagcaggta
    24421 aacaaagtta tccgcgaggc aagactcttt gtgcaagaat ttggtcgtga tccgactaat
    24481 gaagaaattg cagaacgttt aggttggcct gttcaaaaag taaagtctgt taagaatgtg
    24541 ggtagagagc ctatatcact tgagatccca gttggttcag aagaagattc tgaattagga
    24601 gattttatcg aagataagga agtagaatcg ccactcaatt ctgctgcagg aagtatttta
    24661 gccgaacaga ttcgtcaggt tttgcataca ttacctgcac gggagcaaaa agtaattcgt
    24721 atgcgatttg gtcttgatga tggatatgcg caaactctag aagaggttgg gtatcaattc
    24781 aaagtaacaa gagaaaggat tcgtcaaatt gaggcgaagg ctcttagaag gttacgtcat
    24841 ccgactagat caaagaaact taaggattat attctagatt aaaaaaatac gggctagtga
    24901 aaaaattgca ggagtctacc tatattgctt acgcaataga tattttttac tagccctaaa
    24961 atcagacaca caatttatct atataaatta tcgtctggta ggaaaattta gaacgaaact
    25021 ttttttatca gggaagtgat tttatggttc atggttttag gaattgtgtg tatcaaatga
    25081 aaaatatttt taaaaatttt aaaaaaagct tagtgctttt aacaaaacta ccgaaagcca
    25141 tttttctttt ctcaatttta tttttgctga atgcctgttt tgaatatgag gaaactatta
    25201 attttaaaaa aggattttcc ggttttgtag aaataacata cacagttcca ctcaatcata
    25261 aatcagataa ttcagttatt aaatttctac caatttatga ggaagatatt aatcgcagaa
    25321 ttaacaaagg aatattttca aaaaatttaa agataaaaga ttattccctc aaatatttag
    25381 agaaaaacga aaaagaaaca aatcctcttt ttcagaaaaa agctagagtg aattataaaa
    25441 ttgaatttaa tgatctatca acattagatg gagtattgct aggttcttta tttgtaaaaa
    25501 agaaatcctc taatagtatc agcataaaaa gagaatttaa atccgttcta aagccaatcg
    25561 atcaaacttc cacaacaggt gaaaagaaaa ttatttccga gtcgactcgt cttctcggcg
    25621 atggttatat ttcttttcga gtaaattatc cattagcttc tgaatgtaga tccaataaag
    25681 gagaattaag ccttggttct cttttttata aaattcctct tgttgataca attgaaaaac
    25741 caggaccaca atcttgggat tatacaatta caactttata ctaaagcctg attatttttg
    25801 tgagtccaca aaatacatat caatctcacc tttatttttt gccgctactt tgccacggta
    25861 ttcacaatta aaattatctt taattagttc ataggtgctt ccagaaatat ttaccctgcc
    25921 aggtgagcca gaagattcca ttctgcttgc gatattaact gagtctcccc atacatcgta
    25981 acagaatttt ttagttccca cgacccctgc aaccaaagga cctgaatgaa ttcctattct
    26041 tatttcccaa ggaggaattc ctttttgaga gttgagtgta tttacaatat tcatatagtc
    26101 tgctaattca agacctgctt ttacgatatt gatagcatga ttttcatttg cttccggaat
    26161 accggatacg cacatatagg catcgcctat cgttttgatt ttttcaattt tataaatttg
    26221 catgatagaa tcaaaatagg aaaaaaattg atctaattgg tgaattaatt cttctgttgt
    26281 aaatttttca gaaagctttg taaaaccttt aaaatcggta aataaaatac ttgctgtttc
    26341 ataaaatatt ggtttcgttt tcccagtttc ttttaactct ttcgcaacac gggatggtaa
    26401 aatatttaat aaaacttctt ctgtttcttt tttactcaag tcgagagact tgtttagttt
    26461 tttcaactca atttgtgcgt ttactaactc attgtattgt ttaagagaat tttcataagt
    26521 agaaagaagc atattcaaga tatgtaatct gtctgcaaca atttcaaatg ttttccctgc
    26581 ataatttata tgaataggcg ttacttcttc attcgtagag ataggattgc tgagaagtgc
    26641 ttccactcta tacattaaat atttcttgtc ataaggtttt gtgacgtaac catcagctcc
    26701 acattcaatc ccagtcataa tgtcttcagg tctagataaa gaagaacaaa taacaacagg
    26761 tatatctttt aacgcttcgt tacttttaat ttttcggcag agttcaaatc cattcatatc
    26821 aggcatttct acatcggtta tgattagatt cggtttttct ttttcgacta acgcatatgc
    26881 ttgggctcca tttttgcccc aaaaaacagt atatccctca tctagtaata tacgttttag
    26941 catgactcct tgcactgcgc tgtcttcagc gaatacaact gttggcatat tacatatatc
    27001 ctaaaatatg tgctgcgatt ttatcgactg gtagctgaat atcgactgca ccaatttcct
    27061 tggcaacctt tggcattcca tacactacac aactcgattc agcttctccg atagtcttac
    27121 cacccgtatg tttaattgta agtaagccag tagctccatc atcacccatt cctgtgagta
    27181 aaacggcaag agctttatct ccagaatact ttgcaacaga tttaaacatg acatttacag
    27241 aaggacaatg accgttaaac ggtggttcat ttgatacacg aagttttcca tcaggtccaa
    27301 tgatcatatg cttcatgttt tgcggaaaat acacggttcc aggtagaggt gtttctccgt
    27361 cttgcataat ttttactttt agttttgtat tttgtcccaa ccaaccgacg aggctatcaa
    27421 taaagccatc actgatatgt tggacgcaaa gaattggaac agatatatct ctagggagtt
    27481 tttccagtat acttaaaatt gcatttggtc cgccggttga cgctccaatg gcaagcaaat
    27541 tgatttttga ttttatttgg cttgctacaa ctggatggct tgctgttgtc tctcctggat
    27601 tttttttgat ttttctataa acagggatgc ttttaatgat acgtatttta gaaacaagtt
    27661 tatctcggat taaatcatat tctttgtctg ttcctcctgc tggctttgga aaaacatcca
    27721 aggcacctgc ttctaaaagt tgaaatacgt ttgtttcatc tgctccttgc agagctgcac
    27781 taattacaag aattggtttg ggattagtct ccattaattt tttagtaaat tctaatccat
    27841 ccattacagg catcattaag tctgtgcaaa caacgtccgg atttagaaag tttacttgct
    27901 caagcccctc cgctccgttt ttagcagagc caattactaa aacttcagga cttttttcca
    27961 atattctctt tagtatattt aatacgatag gcgaatcatc gactagtaaa acttttattt
    28021 tattatctgt cacttgagcc tctttacgat ttgtaataaa tcctttctat caaattttgt
    28081 tttaacaaga tatgcatctg ctccagcatc catcccttgt tttatatttt cttcacttcc
    28141 aagggaagta aggatgataa caggcgtctc tgcatacgta tttgaatttt ttattctctt
    28201 ggtaaattct aatccattta attttggcat ttccatatcc gtgactacta aatcaaaatt
    28261 accagatttt aatttagtcc aaccatcttc tccatcgatc ccaacttcta ctatataacc
    28321 ctctgactct aatatacgct ttatttggac tcttgtggta agggagtcgt ctacaactaa
    28381 agcatggatt tttttacttt ctatttctag tgtttctaca gccactgtca tatttgtttt
    28441 tgtgatggtc ttcatcaatt cttttggatt taatacaata catatttctc ctgaatctag
    28501 aatggtagca ccagaaacat ttctaacttt ttttagaatt ccatcgaaag acttgatgat
    28561 cacttcattt ttctctatga ctgcatccac ggcaagagct agtctcatac catctgattg
    28621 aataaataaa catggtgttg gggcattcaa tttttttggt ttgatttgag agatttccaa
    28681 atattgagaa agttgaacta aatgcactgg ctgtttttca atgattacag tgctacgtcc
    28741 ttctaaaata aagatatcct ttgggtgaat ggttttactt aatacaatgt tctcagttgg
    28801 aaatccaaat ttttgattac caactgagat gattaaaata tgtgttgttg aaaatttgat
    28861 tggcagtttg attcgaaacg ttgtgccctt acctaattct gattctgttt caatatctcc
    28921 tttaaatttt tcaacgaaat ttttaacaac atccattccg acaccacgac cagaaagatt
    28981 tgaaacagcg ctctgggtag aaaaaccatg ctgaaaaata atggaaaaaa ttcttttttg
    29041 atccatttgg tttaattctt cttgggaata taaacctttt tcaagtgctt tgttttttat
    29101 tttttctaca tctagacctt tgccgtcatc ttttatttca ataacgactg tgtcactatg
    29161 actttttcca aaaagcgtaa tagtggctaa ttcaggttta ccgtttttaa ttctttcttc
    29221 tttagcttca atcccatgat caactgcatt tctaagtata tgcatgagag gatcttttat
    29281 ctcctcaaga atttgtttgt cgatcgtcgt ctctccgcca atgatttcta gttgaacatt
    29341 ttttcctgtt tccctactta aatcgcgtat cgttctatga aataggctaa acacattaga
    29401 aagagataac attctaagat tatagattcg tctttctatt ttcatagaag tcatacttaa
    29461 gcgcgttgta tcattgctaa gatttttcct tagctgactt atattctctt ctaatatttc
    29521 gagtttttct gtttgttttg tattgatttc ttgtagttga tttaagaaaa atttatgttt
    29581 aggagaaaga tcattttgat taataaaatt actgagtata tttctagtct cttgaacagt
    29641 tctgtatagt tcttcgtaag aatagtaagc atctcctact tcttttactc gatttagaat
    29701 tcgattctta gtaacaatta actctccagt ttgaatcatg agcgcatcta atttcgaaga
    29761 gtccacacgc atagtatctg tttttttaga atctacttgt tgtttatttt tatctgtttc
    29821 aatttccttt ttagcggaag gagtatccac taccgatgct tttttacttt caattatttt
    29881 atctatatca ctattaattg gttcttcttt gggagggttt ggttttataa cagaatttgg
    29941 cggagtttct agtttgggtt tttcttcgat tttttcttct gacttaattt ctttctcagg
    30001 agtttgaatg atctcttctt ttggttttga ttcttgagtt tccttttttt ctattgttgt
    30061 aattacttgt ggctttcctc cttttgagtc agtcacagga gcaaaattat ttttactttc
    30121 cagtaatcta tctagaaccg ttactacatc aatacctgaa ctctctcctg ttacagcttc
    30181 atttacaagt ttctttattg catcgagtcc ctcataatag atatctatat gattcgatgt
    30241 gatttgaact tcccctcgac ttgcttttcc taaaacttct tctagtatgt gtgcgataga
    30301 ctctatatca gaaataccca gcatgcgact cgctcctttc atgctgtgtg cttctctgaa
    30361 aattaggtga agcatatcat agtcttgtgg atttttttcc aatgacaaaa gacatttttc
    30421 aataacttga atatgctctt cactttctat ttgaaagaga tctcttaatt cttggtcttc
    30481 tatcatagtt cccataaaat tctagattat ttctcacctt ctactagatc ttttaatttt
    30541 ttagtagcgt ctccaatttg actgacgcct acctttgttt gtgcgattcc acttgcagaa
    30601 tcctgagaac ctcgattgat tgagttcatt gcacttacta tttcattcat agaaatagat
    30661 tgctgtctaa tgttaataga gattttttca atactatcga acactgcgct cacagaatga
    30721 ttaataccag agagtgaatc caaaactttt gatccaagtt tagaactttc atctgccttt
    30781 ttgactcctt cttcggtagc catcactgta gaatccgttg cttttttgat ttcagaaatg
    30841 atttcctgaa tctttaaggc ggatttttta ctttcatctg ctagttttct tatttcaact
    30901 gctaccactt caaaaccttt tccattttct ccggcacgag ctgcttctac ggctgcattt
    30961 aatgcaagca tattggtttc atttgcgagt tcactgacta atccaatgat attaccaatt
    31021 tgacttgttt tttcagacaa acgtaagatt tgttcagcta taccagagac ttttaacttt
    31081 aaggaatcaa tagaatggat catttccatt acagcattgt gaccgttaac tgctagatct
    31141 tgtgcatctt ttgctttcat tgtaacaact tgcgattctt ctgctgatag acgagaagag
    31201 tttccaatat tactaatcgt tgttgtagtt tcattaactg atgcagactg catattcgct
    31261 gttctttcat gattatcaat gaccgaactt atctctcgga tagaagtaga tagaatgttt
    31321 acagattctt gaattggtct acttagaact ctggaaagga aaaaacttgc tcctgccata
    31381 acaatcaata cgattagtgt aactagaatc atttgtgtct gaaaactact cagtgaagtc
    31441 aaagtttcat cgtaatctaa atcgattaga agagcaaatc taacacccaa caattcaatt
    31501 ggagtatagg ctgaaaatga tttgattcca gtaattcctt tcgtgacagt aattcctgat
    31561 tctccattaa aagctttttt tacatggtct gtatcatatt tcttttttaa tatgactgag
    31621 tcttttgtgt tctcttcata atcagttcta agtaaataat ctccaccaac caagtaggat
    31681 atttcttttc tgtctttgat aagagatctt cttatcttat tactaatttc gtctaacgga
    31741 agttgaaaaa ctaaaattcc ttccagtcta ccatcgataa agataccggt tccaataaaa
    31801 gaattaactt gattattcga agcagcgtaa ggctcatagt ccacaaattg tgcctgttgt
    31861 cctccatccg gtgagttcaa aatttttcta taaagcttac ctagattact atctttgtat
    31921 ggaccgtaaa ccaggcttgt catatagtca tcttcttttg caacagtaaa aagaatattc
    31981 cctgtctttg catcaattag aaaagcatcg taataatttc tttcttttat aatggctgaa
    32041 aagaaagaaa ctgcttgtcc atgtgctcgg ctgtattcgg aaccatcagc ttctaataga
    32101 ttttgtttgc ttcccggtgg gtttggatta ttggtaatat aaagggactt atagatatct
    32161 tttgctctat ctaaaccgcc tagtctttcg actgccgttc tcatttctat aaatctttta
    32221 gtagctgcta cactttttct aaagataaat aagtctctta ttttaaattc aaaatattct
    32281 aaaaattcat ttttcttcac atctcgaatc agttcgattt gttgattcgc acttgaatat
    32341 ataatacttt ttcctataaa gtgacctggt atcgatattg ataaggcaac agtaagcgtt
    32401 gatataactg taaaaaagat cagccttgct gtaaaatcca ttctatgtat aaaagctagt
    32461 aacattcttt tctccctaat ttatatattc ttccacataa agtttttcgt aagcgaagat
    32521 tttttcggta tcaactaagc ctaccgtaaa agtgtcatat agacaaagtc ctttcattag
    32581 attttctcct aaactatttt tatctggagg agttgaaaga atttgatctt tagataagta
    32641 tatgacgtct attaactgat ctactaatag tccgagtgtg acttcgttga aacgaataac
    32701 gacgattttt ttattttcaa tagattctgt tttttctgat ttgagtagat acaaaaggtc
    32761 aattaaaacc aacatatctc ctcttaaatt tatacaaccg gaaatatgtt ctggaacgca
    32821 aggaattctg gtaattcctt ctccatgagc aaattcaata atgtatttta attcgagtgc
    32881 taaaaactca tcttttaatc gaatcactac aacggagagt aaattagcat attctttgat
    32941 ttctgctgat tgtgaataat tctttctgcg gctaattaag atttgcctat ctacttcgct
    33001 gagtcctcca aatagctttg cgaggtgaat gtcagtgaag gacttaattt tttttccatc
    33061 cgcatttgga atgtttaaat tactcaaggc atcaatgtta atgatctgag tgattccaaa
    33121 ctctgtttga atcaccccat ccgttagtcg ttcgggatca gaatcttctc ctgaatctgg
    33181 tttttcaaat ggaaggtgga ttacattgtg cacatcattg atgtgaattc caattttttg
    33241 gtttccggtg cttattacaa gaattcgatc ctgaaccgaa taacgttttg ttttaattcc
    33301 ttgtcgtaaa tttaaatcta tgactggaac aaattcactt ctaaagttaa aatagccaac
    33361 gacaaaaaaa gagctagatt caattctaga aagttcaggt aaccaaatca attctacaac
    33421 acaagcggag gaaattgcat aaagatttgc ttgaacggaa aaaacaagat agtttgtgag
    33481 ttttttatct tctatagcca tatactagat tcttcgctga agaaagcttt taaactttca
    33541 atttgatatc ctttctcgat gtaaacatct cttagtcttg gatcatgttc caaacaaaaa
    33601 agtccagctt ttcgaacttt gatagactct ggaatattgt gtaacaagca gtataaattg
    33661 gataatgtca agtaagattc taaaaaattt ggctctaaat aaattacctt tttatatata
    33721 tcaattgctt gattccaatt tccggctgct tcaataattt gaccctgtag gaataaagct
    33781 tcgtaattag agggatttat atttaataat tcttgaacta atttttctgc ttctatcact
    33841 ttacctagat ctgcaagctc tcgcgcttta tacaaagttt ctttatgatc aatcgaaaga
    33901 atcggtttag tatcgataaa tgttttttct attttcgtat aggtcgtggc tggtttagtt
    33961 ggaatagttg cggataatcc ttgtttgcta ctaaaattta gagttggaat tgggctatgg
    34021 tctttttttt gataaaccat agatttctgc attgggataa ttttaaatgc atttgaaatt
    34081 aaatgagcag cttctgaatg accaatgagt aagtatccat ggttcaaaag tgctttttcg
    34141 aatttctcta aaatcatttt ttttgaatgg tcatcaaaat aaataaatac attcctacaa
    34201 agaattagat caaactcatt gtagtaattt gagtggaata aattgttttc aatgaactga
    34261 actttatttt taatagagtc atttatatga tatccatttt tctttttggt aaagtatttg
    34321 tcctttttat cattggatac atttctaaat gaccactcaa gataatctcc ttggattgct
    34381 tgtcgaattg agtcaccatt tatgtctgtc gctagaatcg atatatccca acccatcatt
    34441 aattcaggaa tctcggctaa cgtaattgcg agtgagtatg gttcttctcc cgttgagcat
    34501 cctgcactcc aaattctaat ttttcttgaa gcttgttttt ccttaataat tgctggtaga
    34561 atattttgtt ccaggatttc aaactgtcca gaatctctta aaaagtaggt ctcggagata
    34621 tttaaggagt cgacaataat tttccattcc tcgttaccta aggttgaact ttccttcaaa
    34681 tactcgatgt atttttcagg tgagatcttt tttagtaaat tggatttctt tcgaagtgta
    34741 tctaagtaat ggtctatttt atccgcagta acatgaattc ctaatctaga ttgaattatt
    34801 tgggaaatat ctatagcaaa ttgattcata ttaaagctca aacgttattt tgctaaattt
    34861 tttttgcatt ttgtttaaaa gtgagaaaag aattgaagca atgaatacaa tgtgaatctt
    34921 tttttgacaa gaaaattatt tttcaatcaa gagaaaatac attgcaaatc aagtaaattc
    34981 aatggagtta atctagcttg ttgcaaaaca aatcttttgt cattttttaa tcctttatcc
    35041 tataaaaagt agtggtaaga ttgcaaaaaa ccttaattaa acaacaatct aattctcttg
    35101 acaggctcta tatgtcccaa aacaattcat tagaagctat ttcaatttga ggagacaata
    35161 gtgcgtagca aaacgaaaat ttatgtctta atggcagtct gtatattaat tggcactgtt
    35221 ttatcaccta tcgttttttt tggaaaaaaa gagtctggtc ctatctattt agaagctcaa
    35281 tccggaactc cagtcaagga ttcaccagca aaggctcaag cagttgcatt ggaaaatgca
    35341 tttcaagatg tttttgacac agtttcacaa agtgtcgttt cgatttctac tgaaaaaacc
    35401 gttaaaatta aaaatcaccc tcttgcagcg gatccatttt ttgagcattt ctttggagga
    35461 cctaaacagg gacaagaact caaacaaaaa caaagaggtc ttggatcggg catcattctg
    35521 aacgaagagg gttatatcct aacgaacgaa catgtggcgg gcgatatgga caagttaact
    35581 gtgaaactta aaaataaaaa gacctatgag gcaaaattaa ttggttcaga taagacaatc
    35641 gatttagcat tactcaaaat caaagccgct cctggagatt taaaaccggt cgtgctagga
    35701 gattcatcca aagttaaggt tggaaactgg gcgattgcaa ttggggctcc tcatggttta
    35761 gagcattctt ttactgtagg cgttgtaagt gcaattgcta gaagtggaat tgatgcatcc
    35821 ggacttggat acattcaaac agatgcagcg attaatcatg gaaatagtgg gggacctctt
    35881 ttaaatattc atggagaggt aatcggtatt aaccgcatga tcgtttctcc aagtggcggt
    35941 tctattggta tcggtcttac aattccaatc aatgatgcga agagagtttt cgaggaatta
    36001 aagacaaatg gtaaggtaaa acatgcttgg cttggagtcg gactcgaaac tgtaaatgag
    36061 gaagatataa gagagttgaa actttccgat cctaaaggag ctgtcgttcg agaagtaaag
    36121 aaagattctc ctgccgatca agcaggaatt aaaattcatg atgtgattgt gaaagttgga
    36181 gagaaagatg tggagacaag agaagacgtt attgaagcag ttctcaatac tcgagtagga
    36241 aggaaagtag aagtgaaatt gatacgaaaa ggttccccta tgaaagtgat ggttacaact
    36301 gcagaaagac cattctgagc gaataatcct tgttagccaa tagaatttta aatcctgaag
    36361 ataaaagtga tgacgataac tccctgcgtc cggagaatct aaatgatttt atcggacaaa
    36421 aagaaatact agaaaattta catatcttca ttcaagcggc taaaaaaaga gattcggcat
    36481 tagatcatgt attgctttct ggacctccag gattaggaaa aacgacgtta gctggaatca
    36541 ttgcgaaaga aattggatcc aatttagttg taacatctgc tcaggtgatt tccaagggag
    36601 cagacctagc tcgatttctt acaatgttaa atgaaaaaga tattttattt attgatgaaa
    36661 ttcatagctt gaatcggaac ttagaagaaa ttttatatcc tgccatggaa gactacaaaa
    36721 tagatttagt agtcggggaa ggaattactg ctcagactgt tcagatacca ttgaagaaat
    36781 ttaccttggt tggagccact acacgaaatg gtatgatcag tgaaccgctt cgaaatcgat
    36841 ttggaattca atttaggcta gattattatt cagatgaaga gatggcgcaa attgtttcta
    36901 gatctgctcg cattctaaat attaatattg aaaaagaggc ttgtctagaa atcggcaaga
    36961 gaagtagaaa gaccccgcgt atttcaaatc atttactcaa aagaattcgt gattttgcag
    37021 aagtcaaaaa tgatggacgg attgatttgg aaatttgcaa atcttccctt caaaaattag
    37081 gaatagatca tttgggtcta gataatatgg atagacaaat cctaacttgc atgatagaaa
    37141 gatacaaagg tggacctgta ggtcttaagg caatcgcggt agttgtagga gaagaagaaa
    37201 gaacaataga agattcctat gaatcctatt tagttcgatc tggtttggtt ctcagaactc
    37261 cagcgggaag agttgctagc gagcttgctt acaagcattt aaatctggac aggaaaacaa
    37321 aggatgaaca aagaaatctt ttcttttgat ctaaactcac tcgatgaaga tgaaaggcgt
    37381 cttctattat ccgcgagttt agtattcttt tttctgtctt ttacaatcgc tcatttattt
    37441 acaaaaaata tgctttggag actacttggc actgaaagtg atcttgtaga aatgaaagag
    37501 ggtaaggaaa atcaaaaggt ctatgaagta ttactcgaac aagaatttgt aaataaaaca
    37561 aaaaaagacg aaatcaaggc tctttcgaat gaggactctg ccggaactgg gggactcaca
    37621 gaaaaagaag gatttcacac aaattcacct ttctacgaat ttatattcgg aggattgcct
    37681 agttcctctc ctcaaatggt tcaaaaaaac aatgaatcta aaaaaacaga agatgaaatt
    37741 tatgaagttg gaatctatca aaatgatcca atgatgaaac agactcctca aacaacttct
    37801 gtttccaatc ctgcaacagg acaagaaacg aaaattcctt tcaactatcg gtttcaaaaa
    37861 gatttcgaat ttagatggga tggttccagt caactttcga tccctcgaaa aaaaatggct
    37921 ggctatgaat actttaaaca tatgatcaaa caaattgagg aagcatttgc tgctccaggt
    37981 ggtgggaatt tcgcctatcg tgatatggca ggaaccgtaa ttcgagaagg aattactcct
    38041 ggggaaacaa aagtgagctt tatgttaaat gatgccggac aagtcattga tgtaaaacaa
    38101 atgtcttctc agggacaaga gattgtagat agagcgtgta tcgactcaat tcgaggacaa
    38161 aactttggtc ctgttccccc tgaagtaaaa gaacaaggta tgatctttgg aattaacttt
    38221 gtatttcctg gatatgttcg ctatagataa ttgcgacatt ctaaattaga ataaaatttt
    38281 ttaaattaaa ttataaacct cgaaggatgc gaaatgggaa agctaatcgt ttttaatttt
    38341 attactttaa atggtaatta caaagattcc ataggggata caagctggca ccgacatgga
    38401 gaagaagaat ctgaatatgc aatggagaat atgaagtcag ataatatttt actctttgga
    38461 agagtaacgt atgagatgat ggccgggtat tgggcaagtc cagcggcaaa acagaattca
    38521 cctcaaatgg caaagagtat gaatgatgcg gagaaatatg ttttctcaaa gaaacttaaa
    38581 aagacaactt ggaaaaattc aaaaatacta aaaggaaatt tggagaaaga aattctagct
    38641 ctcaaacaga aatccaaaaa agatatagta attttaggaa gtggaagcct tgctgctagc
    38701 tgtgcatcgc ttggactcat cgatgagtat cagattatga ttgatccagt tcttttagat
    38761 ggagcccaaa ttttcaaagg catttccaat aaaatcgatc ttcgcttaac gcattctaga
    38821 gtttttcaga aaagtggagt ggttttactt tgttatgaag ttattccaag tagaattcaa
    38881 aaaataaaga tagaaaaaga tatcaatgtt ttttatgtaa aagcaaaatc ctttccagag
    38941 ggaattcatg atgcaattga tacattgcaa acattgactc caaaatctaa aaagcgtcgg
    39001 tttttcggaa tatcgaatcc agataaaaaa ggagtgattc aatataaagc ttgcgcagaa
    39061 gaattaaaaa agcaggaagg aaaacgctta ggtctagaaa cttttagaat taaaaaaggg
    39121 atgtatttaa gtattctagt taaagacttt caaaaagatc caatgcaaat tggaagcgca
    39181 tttcaagaaa tgctttttag gcctgagatt gatccgaaag gatattgcct tgaatggtat
    39241 caaaatgaaa ccgatgtttt gtgtttggtt ccgataggct aatttcgttt atctttaaaa
    39301 aaaacttgca caattttttc tacatcctct gaatacagaa acgttagttt aggaaaatga
    39361 tttttagaat agatcgattc aaaacttaaa gatgtaatgc ctgctgtttt agttgcttct
    39421 agaaaataaa tgacttcttc aatccttgca tgaatgatag cacctgcaca catgatacat
    39481 ggctcgattg tagttagcaa aatcgattga ttcaaaaatt tagtttgtaa ttttttttgt
    39541 gcagattcta tacataagat ttcactgtgt ttatacgttg ttttattttc ctctacttgg
    39601 ttaaatgctt ccgataccaa gcttccttct agataaattt gagagaaaga tggaatttct
    39661 tctggatgat ttcgggtaat ggaaataaat ttttctaaga aagtttggat catttgtctt
    39721 cgaaggaaga tacaccttcc caatccagtg gaactccgtg tttttcataa taagtgcatc
    39781 tttttaaata taaaagagct gcattatcat gtgggttaat atcggaaatt tctttaaaac
    39841 agctaatcgc gtcatgaaaa tcttttgtta aataaagact tactcccatt tcaaattgag
    39901 tttttgttgc tttaaataac ccaaaaactt cttctgaatg accatctagt atttcgacta
    39961 ccgttagatt cacggatttg ccttttacct taactctatc taaaattctg tataagtatt
    40021 tatccgcatc ggtcaaagaa aaaagagttt cgatactcat taaaatagat gctccataaa
    40081 ttttggtaag accttcaatt ctggaagtta agttgacagc atcggaaata acggttcctt
    40141 ccattctatc ttcagttcca atcgttccta gcatgatagt tcctgtatga attccaattc
    40201 ctacctcgat tggtttatag ttctttactt ttctatgatc attgtaatgc ctgattgctt
    40261 tttgcatctc gatggctgat tcgattgcat catctggact tacaggatag agagccatta
    40321 gaccatcacc aatgtattta tcaataaatc cattgtgaaa ttgcacacaa ggattcattc
    40381 tttttaagta agcatttaaa aaattgaagt tctcttcagg gctcattgtt tcggagagtg
    40441 ttgtgaaatc acggatatca gaaaagagaa ttgtcatctc tttttgaatt tggtttccta
    40501 actcaacttc caaaatactt tcttttttta aatataaaat aagttcatag ggaacaaatc
    40561 tagcataggc aaaattggtt cgttttaaat cgcctgataa ttgttctatt cgtttgaaag
    40621 cagaagaaaa ttttaagagt agcgcataag actgagtaac tgccatgatt gcaattccat
    40681 aaggaactaa gaaaagattt gttgtgatga tttcatttgc ggcaaataga tcatgaatat
    40741 ttgtaagcat aatcgcaatt gtgcttacaa atatggagag tgcatattct cgtttattta
    40801 gaactgctag gaataaataa tacggaatta aaatgagtgc tacaagtata aatggttgta
    40861 gaattaaaaa catataggta atatgttttg tttttagaaa tagactgaaa cttgctaaaa
    40921 gaccaatacc atagtagcaa agaaaaagta atctgtgaat ttcttttgga aatagagaat
    40981 gtaaaacatg aattgctaaa gcgggcgccc aaaatagaat taaatattca aaacgtagat
    41041 acacatcgta actttggaac aatacataat ttgttatgag tttttccccg cttgtaataa
    41101 tccgaagaaa taagagtaag aaaaagaagg aaaatagaat cgttgatttg tcattctttc
    41161 taagttgata aaaccctaaa tggtagatga agataaataa aagtagtcca gcaagaaatg
    41221 aatctgtttc tcttgcactt ttttctagtc cactgatctg atagtaattt cccaaataaa
    41281 gactatgcca tacaccgcct ttgcgatgat gaaaattaga aatatgcatg actatttcca
    41341 tgtcttccat ttttaaatcg ggaacatatc cgatggcagg gacggtatgc ggaatagatt
    41401 ctttttcatt tttaccaacc actccagaac caagggtgga tactccattt acataaatgt
    41461 gaaatgcagt tgcttgttgt ttagaacgaa tggctagggc tggactattt ggaggtaata
    41521 aaatttttaa tcgaaaagtg gcatatccat ctgatccaat agtttctcca tttataattt
    41581 tattgttcca ggagtgtgga aggtgaatgt attctttatt gaaaatgata ctatgaatct
    41641 cttcgagaga gtataatttt tcccaataaa attcccattc cccatctagc tcaattggac
    41701 cgtatttatt aaaatcccat tctcttagat ctagaattcc tttttttgcc agagttttat
    41761 ttttattgcc agttagattt gataaatcac aggaattaaa aaatagaatt atgaagagta
    41821 taatccctat tatcctatga tttttcattt tattttaaac tcatatcctt aaaagttaaa
    41881 tttgagtgtg aatcaaatta tatagagctt tgtttttatg gagtatcgtc atctgcatcc
    41941 atttcctcta cgttaggctc ctcatttgca tcagcaggtt tcttttcttc ttttgcggga
    42001 gcattggctt cttcctttaa aagttgcaaa cattcttttg agtatttctt agctctctct
    42061 gtcaatggac taggttcata tttatctaca atcgcattaa atgcggctgc tgctctatca
    42121 tatttctttg aggaaaaaag agccatacct tttgtatata aagcatagtg atcgagtgag
    42181 cttggttcat ttaccaaaac ttcttctgca tatttaattg cttctggata acgtcctaat
    42241 tttaaattgg attctcctat atagtagagt attttctctt tttgtttacc tgctggtgta
    42301 attctaagta atcgagtaaa tgcttttatt gctttgtcat attgagcttt gttgtaaaga
    42361 gtttttgctc tatcaaaact agtttgcttg tcctctgctg gaggaggagt ttttacttct
    42421 tctttgtcga tatttgcaat ctcttctgag taattttcaa aaatatcata agaggcaaag
    42481 tctctgccta gttttcttaa agctctcgtc catccaattc tagcttggac attgttaggg
    42541 tattcctgaa ctgctaaaaa ataattttta ctcgcatttt catatctgcc ttttctcata
    42601 taaaaatcag caatcgaaag aagagccttg gatctaagct gactgtcttt gctcgtttgc
    42661 agaactgaat ttaaatgaat ttttccttct tcttcttgat tgagtaaaag cagaacattt
    42721 ccaagtgcgt aacttaagct ttctctttct tctttggtta gattagacat ttgatttaat
    42781 tctttataaa tgtctaacgc acgaagtctg tctttatttt tttgtagagc tccggcgtga
    42841 ccaaagcgaa ctctaaattg aaatttctct ggaatggatt ttgaagatag ctctgtaaat
    42901 aataatagag ccttttgatt gcttttctta cttgattgat tcaagagttc ttctgcaatt
    42961 tttaatctct cttcaaatgg ataggcgtta ttttccactg tcttttcttc tgcatgattt
    43021 accattacat aaacgccggt caatactcct gcagctacaa atagaaatcc aactaaaact
    43081 aaaattaatc tactcattta tttccccgat tttccaaaca tctttttaca taaaaatcaa
    43141 cacgttctct atcataattc atctgcacat cttcacttag aagaatcttt aatgcagcac
    43201 tataatcttt taaattgtaa ttacacaaag cagcgaaaaa cagcgatttg ccgcgcagtg
    43261 agttgtttga aactctatcc gcaagtgatt taaattttac aagcgcttct gtgtattttt
    43321 cttttttata atattgcgtc ataattgttt cgaaatctgg attcgaatca tcagttacta
    43381 tttgtggttg tggagttggc gtttttttct cttcaactac tttagcctct tctgttttca
    43441 cttcatcgga tttctttttt ttctttttac gtttactcaa atctaattta ataaaggatt
    43501 gatcttcttt taaaactaga ttttcttctt ccgagccatt ctgaagagtt actgcataat
    43561 aaaaaattcc ttttcgatcg agccttggat gaagaaaggt aagatcagga tgaacgatag
    43621 ttcctatttt ctttgcgcct tcaatgagtt tactgtcagt atcatttgca attttggaac
    43681 tagattcata taaaaaatac tttgtagtat tattcaccac tccttcttgg ggaatccaag
    43741 taaataaaat tccatcttta cgaagtttag cactgagttt agtaatcgat agtggttttt
    43801 tattcgcagg aacttgaatt ggatcttttt tctcttccac aattttaaca ggttcatctg
    43861 ttttgatata atagaatttt ttaaaggatt tgtcttctgt cagagggatc gattctttct
    43921 cgcctattgt aactgttacc ccataataaa cagtctgtgt agaatcaatt gtcgtatcta
    43981 gaaaagtagt ttcaggatgc gttgtttctc ccaatttggt tgccttttcc attaagcctt
    44041 catttgacat tggatcagga gatcgataga cggtatactt aattgaattt ttatcagcat
    44101 caatcggagg tgtccatgtt aggcgaactg tattgtctat cttcctgact tttaaattac
    44161 taatcgaatc aatttcatgt ttaacttctg caatcgctga aactggaatc ggattttctg
    44221 gtctagctgg aacgataact ggattcactg tgtaattgat ccctggatag agttttactc
    44281 ttttcttttt tacttgtgag accaatacga ttgcataata atattcaccg gctcttaggt
    44341 ttatatcttt gtaagaatta aaaacattta ctttatcact tttatatttt ccaagagaat
    44401 ctgagattcc tagtttttct actgtatcta ttacagagtt ggatctggca attactacat
    44461 cgccctcttc ttggggaggc gtccatttga gttctattga aatcgaatcg ggactgagtg
    44521 ttgcggtaag atcggagaca atatctctct ccactttttt cttgtcttgg ctagaagtat
    44581 cctctgtttg tggggatagt ggatttataa ataagaaaac aataataaga aaaaggatag
    44641 aaatccatcc tagattgaga aaagggttta ttttgtttat agaatgtttc atagttttta
    44701 ttgttggaaa gaaaaacaaa aatagaacat taggaaataa atttcaacca tatttattca
    44761 tttcatcatt ctaattttat tgtcgacatt taagagatta gattgtaggg tcgactagac
    44821 ataacaagga aaataatact atgattagcc aggaaattga ttattacgag gatatgaagt
    44881 atcaggactt tcttatgtcc tctcgaagaa aagtaatttg tccgccagag aaaattatgt
    44941 attatttaga aattaaggac gctatgcatg tggcggattt tggtatggga aaagggtttt
    45001 ttattcccta tttaaaaaag aaaatgaata aagaagccca tctttgggga attgattacc
    45061 aacaagaaat attagattta gtcctcaaaa gaaaagtaga agaaaatatt cagaattttt
    45121 cagtggttca cattgataaa acagagcacc cacttttacc aaattggatt ccacttccag
    45181 atgttgtatt tagtgcaatg tgcctttcta cttttccaga tccgggtctt gcaatggacg
    45241 gactcattcg ttctatgaaa ccagaaggaa gattatttgt aatcgattgg gcaaaggtgg
    45301 agtttgcgga aggaccagcc attaaagata aaatctcctt tgataaaatg aaatacctag
    45361 ctgagtttta taaactaaaa gtggtaaatg catttacaat taatgagttt gtgtatggaa
    45421 tggagatagt tgcgggacca gaatttaaga ctcaatttta cgattatagg gaatagaaca
    45481 atgaaagaaa ataaagtttt agaactactc aataagaaag attgcacaat tgcgattgtg
    45541 ggagcgacta acgattcaag taaatatgga aatattatct ataaagactt gaaaggtaag
    45601 aatataaaag tctatggaat caatccgaag gcaactacaa ttgatggaga taaggcttat
    45661 catacgttag atgatctcgg gtttaaacca gatattattg acatggtgat tcctcctaaa
    45721 cttggtcttg ctttaattca agatgcagtc tctaaaggtt acgataattt ctggctacaa
    45781 cctggtgcgg agagtgatga gatcattcag ttcttagagg ataaaggtaa gaattatcta
    45841 gcttattctt gtgtaatggt tgagagtaga taatgaataa taattacacc gattcgattt
    45901 taaaagattt agaaaataaa gaaaatggtt cctttcagat aaaaaatgaa aacaatcatg
    45961 cagtgcttac tgtatttaag cctggaaaaa atgggcgagc tgttactaga caagaagtct
    46021 tccaaagact aaaacttttt agcatcgaag gatacgatag ggcacaggta gaagtaattg
    46081 ttgcaaatgc agatggaaaa gatcatccaa ttgcaatttg gacgggtgga aatccagttg
    46141 attcaagagt ggaattggaa gtggaacctg ataaaatgag tgcctatgta attatctatc
    46201 ctgctcttca cggtggaaaa attcttaaga aagcagatgt aatcgatatt cttaaggcaa
    46261 atggaattgt atttggttta aaagaagacg ttattaacca tatattagat aataataaat
    46321 tttttcagaa aactcttgtg gcagaaggat taaagccaac tcctagtgta aatggatata
    46381 taaaggtttt atttgagcca catagcaaac cgaatttaca cgaagatgca aagggtcgga
    46441 tcgactttaa agacatacaa gtaattaaga atacaaaaca gggagatgtt cttgctgaga
    46501 aagttgatcc aaagattgga actcccggaa aaaatatttt cagtgaagag gttactcttg
    46561 ataagcccct cgaaggggaa tggaagattg gaattaattg cactttatca gatgacaaaa
    46621 agaaattgca tgcatcgatt gcaggaagac ctattttaga aagagatgga acaatccgtg
    46681 tagatgaggt ctgttattta gaaaatgtag attactctac tgggaatgta gattttccag
    46741 gaacgattat cgtagatggg actgttgcgg ataacttttc tcttagaacg aaaggatctc
    46801 tcatggttaa gaaaagtgta gggagagttt ttctctatgc agagagagat attgttctat
    46861 caggtggggt gatgggaaga aatgggggcg tgatcgaatc caaagcagat gtctatgcta
    46921 ggtttgtaga acaaggcaat atcaaagcag gaaaaaacat catcattgaa gaggcatccc
    46981 ttcactctga attagttgca ggcgattcca tcgtgatcaa aggtgggcgg ggagaattga
    47041 taggcgggga agcgatcgca ggtaaatcaa tctctgttag taaactagga gccgttgtag
    47101 aaactaaaac agaagtagtt gttggccttc ctcctgagat tttagatgaa cttagaaaaa
    47161 tgaaagatga gattgcttct ttcgaggaag tgttaaagaa agtaaagcaa actatggcaa
    47221 ggctttcgga tccgaagaaa gaattaaccc aagaagagaa gcaaatgctt actaagcttt
    47281 atgaagtaga aagaaaatac aatgatttac ttgtaaattc caagactcaa tatgaaactc
    47341 tcgttgtaag ttatgaacct gcaccttccg cttttgtaga agtagaaaag acgttatttc
    47401 ctaaagtttc tgtcaattta ggaaaaggaa aaatctatca ttctgaactt aagatttacg
    47461 aaggcaaatg ctatatctac aatggagatg atggaattcc tatgcgtagt cctgtgcctc
    47521 ctaagaagaa agatgcagaa taggaatgtg taaaacaatc aaacaaaaag taaaattcaa
    47581 agcttctcct gaagtaatct atgcactttt agcaaactca aaaaagcatt cagcctttac
    47641 gggtgaaaaa gcaatgatta gcaataagat tggcggaaac ttttcagcat acgatggata
    47701 tattacaggg gttaatgttg atttgctccc atccaaaaga atcgtccaag cttggagggg
    47761 aagtgatttc ccagaaggaa ttttttccat ggcagccttt gttttggttt ctacaaagga
    47821 gggaggaaca gaacttacac tcacacatcg aggagttcca aagaatttaa ttccatccat
    47881 agagaaaggc tggaaagaat tttattggga taaaatgaaa gattatctag cacagaagaa
    47941 ataggaatta accttagttt tttaccaccg ataacaccga taggcaccga tgagttgatg
    48001 atcgaggtta tgattattaa ataatcgtag aggcagatca ggatctaact acgttacgtt
    48061 acgataattt aattcatcgt tccaattttt caaccatact tcgactcctc tcagtataaa
    48121 ctccgcacac gaatagacac gaagggttga ggtagggtta tgatcctgta taaattatgt
    48181 agggcagttc aggtgacttg atgtttcctg taatgtgaat catcaatacg attcttgaag
    48241 caatcgttat ggaaatctat taacgataca ctaaacatcc cttaaactta gggtaaactt
    48301 ctcctaaaga taccctcgga gataagttta ggggaacttt acccaatctt taccgctaag
    48361 tttaggagat gtttggtgat atgagaatta aaagattacc accgatagac accgattaac
    48421 accgagaggc accgatgagt tctggttgtt ttaattctgt tcatatataa aacgtagagg
    48481 cagatcagga tctggttctg ttacgtttgc caaacgagaa atccaattta aaaagaaaaa
    48541 tatttaaaag attttaacca catgcgaagg cgtaggtcgc gaatacttgg agcgaagggt
    48601 ggaataaaca ccgagagaca cgaagggttt taaaaaagat tacagtgggg agtatgtttg
    48661 aattttgttc ttccaataac gtttcagtat taacaaatat ttgaattcct tttatttctt
    48721 taacgtttca aagtgattct ctgcaaattt tcgttaaagc tattttgaaa atttatactt
    48781 tcaggtttaa gtagaatcta gagttttttt gattgaaatt aatgataagc gagaaattac
    48841 ttcgtataac atgcgaagta attggtgtta tatgaaataa tcctgttgac atgttattag
    48901 aaattcagag aagtaacgtt atgtgccatg ccactggctt tctattttta ggacaatttt
    48961 ttagaaaaga atttaaaaga ggattggatt tttgcatttg ctcaataagt tttgattgat
    49021 ttacgaaagc tgataattat ggatttagaa aaaaattaga attaagagaa ctttgaaaaa
    49081 agaaattgaa aaataaagcg ttctaagact ggaattttaa cgactaagaa gaaaagtaaa
    49141 aaaagagatt aggagtaaat agaataagta gttctaaaaa ttttcttata atgtaattat
    49201 tcacttgact gcatatttag aaaagtgata atgtaaagac gtgtggaaaa tatatagaac
    49261 agatgagttt aaagattggt tcgattcgct agaagaaatt tcaattactg cgattgtgac
    49321 ggatttgttg atactagaaa aatatggacc aagcctcggc agaccgtatg ttgatacact
    49381 gaaaggttct aaaatttcta atttaaaaga attaagaact cgttcaaaga gcggagtatt
    49441 tagaattctt tttgttctcg accccgaaag agatattatc cttctcttag gtggagataa
    49501 aagtaatgat aaaaagtttt accagaaaat gattactaaa gcagaaaagt tatatcaaga
    49561 acatttacga aacttaggga gaataaaaaa tgaagattaa caaaaagaaa gactttattt
    49621 cagaacttcg agattcaatt ccaaaagatg tttttatcaa agctgaaaaa aaagctcaaa
    49681 gagaaatctt cttgattgaa cttcgagaat tgagaaaaca ttttggagtt acccaacaga
    49741 aatttaaaaa ctttactcaa tccggtattt ctaaactgga aagtagaaat gatatgaaat
    49801 tatcaacctt aatcgactat atggatgata ttggattagg tatggaaatt aaaatttatc
    49861 ctaagaaaaa taaaaagaaa gaagaaaagg aatttgtctt actgaaaacc taattgtgta
    49921 agaattactt ttaaagagta gaataagtag agaagttaag ctttattgta tttgcggacg
    49981 tggcacggac acataacaaa gagtagccgc taagagagtg aactaagttt ggatgctgtt
    50041 cactcccttg ctccgagccg tctggttgtt taagtgaaat tttttagttt atttggaaag
    50101 c
//
LOCUS       DAOQOS010000030        49774 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_269_length_49774_cov_5.530602, whole genome shotgun sequence.
ACCESSION   DAOQOS010000030 DAOQOS010000000
VERSION     DAOQOS010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 49774)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 49774)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49774
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_269_length_49774_cov_5.530602"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            493..2829
                     /locus_tag="PLF66_08590"
     CDS             493..2829
                     /locus_tag="PLF66_08590"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HMZ64136.1"
                     /translation="MKNQFGYATYRLKIILPENSKNHQLSLRLVNLLTASNVYVNDIL
                     IYKSGKISKTPENSEAQILPASVNLPQLEKEFLLTIQISNFHQNKGGIYSAPELGSQL
                     AISGRREKQVAIDMLLFGSIIIMGIYHIVLLVIRRKDTSNLFFGIFCILLSTRIAIMG
                     ERIFIHWFPKPGYDFYMRLELISYFLAIPSFTLYVKSLYPNLIGKWIAVVSIFFGVLY
                     SLISILTHASIFTNLIFQSSLILIFIGIYIIIAITRAWFKKLQGAKSFLFGFFILFLS
                     SLNDIVNSLTNEFNTGIYIVPVGLLIFIFSQSYLLSVRYSNAFQKIEELSSNLFELNT
                     TLEQKVINRTKELHSTLTEVQNLKEKQDADYFLTSLLIDPLKTHDITGDKVKIEFKIQ
                     EKKQFTYQNNTKGIGGDLCLAKTIILKNRSYSLFVNADAMGKSLMGAGGVIVLGSVMK
                     ACIERTIQTPTEKLLYPERWLKNIFLELHKIFESFNGYMLTSIVIGLVDEKTGLVYFI
                     NAEHPYVILYRSQKAKFIESQIHFSKLGIGNEDSTIWIQTFQMKPNDVILVGSDGKDE
                     LLKYDKHGNTSIVSDYKYFLKIVEEANGNIDSIFEILNSKLEQLIDDLSLLRLSYLEN
                     NKPQVQTTIIDKIKDDISKGIILFKNKDYINALNVFQDILKHQPENEIVIRYANACAI
                     KMEDFKLALEYGLQLIEIRPDKSFPIFQTSILARRNNQLLLAADLGERYRLRNPKNIK
                     NLFSLIETHSKMGNHSRIEKLLKEIRTINPNHPLLHKF"
     gene            complement(3204..4694)
                     /locus_tag="PLF66_08595"
     CDS             complement(3204..4694)
                     /locus_tag="PLF66_08595"
                     /inference="COORDINATES: protein motif:HMM:NF024686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FN3 associated domain-containing protein"
                     /protein_id="HMZ64137.1"
                     /translation="MKQLSTKQNTFLITKTYFIILVLFLFGCLQAKKSRFDINSPSGL
                     LFGLVNGNSYSQFISNGTVNSSTPQSNTKDITSYSFLGIAATSSISSNVINLEVPLGT
                     NLSSLVATFTSNGKSVKIGNIDQISGVTANDFSGGIVYTVIAEDSSTKEYQVLVTIQG
                     QVSAPSFNVATGIYSKDQSISLSSATLDANIYFNRGLNPTDPNCSGNGTLYTSPILID
                     ITGTIVKAIACKVGLADSNVSTATYTLKVAQTLVYSNNTFNLLVIPFSFSATTSSVPS
                     ITKCLRANGTPACNSDGSCATGSSTTLSYNTNADVTVSAIGCRANYTPSDIDSKTFKV
                     SAIRSADNSYLTIVDASANTNIMNKKVYICSEGETYNTSTGNCSGTPTDFQFCATNDN
                     SCNGGTDTGQAANGSPIFNYCNIRTYGGFAAGTWRAPTLNELTGFFPIYAKNPNGWPS
                     SLGLNYWSNQSVLSGFANEVSNTGPFQTNIYQAQKTTLKNLRCIRN"
     gene            5570..6853
                     /locus_tag="PLF66_08600"
     CDS             5570..6853
                     /locus_tag="PLF66_08600"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010573487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HMZ64138.1"
                     /translation="MKQINIGLIGAGTVGSGLIEILHSHRKQILDRTGIDLNLVKICD
                     TDTSRIPSYFKGEAVTDFKKITEDPKIQVVVELVGGTGIAYEIVKSALSNGKAVVTAN
                     KALLSEKGPELFQLAEEKKVEIGYEASVGGAIPVIRSIKTGLVANDFQSIFGILNGTT
                     NYILTKMEEDNIGYADALKQAQDLGFAEKDPTFDVEGIDAAHKLSILIGIAFNKKIKI
                     SDIHIEGISKLSEAEIHAAKLLGLRIKLLAVCKQVGDHLEARVEPTMIPLTHPIASVR
                     NEMNAIFFETSFSGPSMLLGKGAGSLPTASAIISDLVFYGSRLNELGEIKEKNLFEEG
                     KLTPPGEIKARYYLRFKTVDRPGVLGELTSIIGRNLVSISSMRQNESNSEPVELIIIT
                     HEALEKDVRNSLMEIDKRSDIIKGKSIMIRLEDLI"
     gene            6850..7173
                     /locus_tag="PLF66_08605"
     CDS             6850..7173
                     /locus_tag="PLF66_08605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64139.1"
                     /translation="MNRCTISEHRLEKEKHLDIFFETEPHLETYEAEVDQFSLLLSQK
                     KIFCRKKQAHRKIYLEYEGEVSANRGNIKILWKGFYENSLSFFLEEIDLFLLKENLLR
                     IDARS"
     gene            7189..7557
                     /locus_tag="PLF66_08610"
     CDS             7189..7557
                     /locus_tag="PLF66_08610"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HMZ64140.1"
                     /translation="MREINFDALVVKERIEVINDTKVQILYFVGQIDQNNSYTISDNI
                     NAFFESGTFNTILDLSNLVYVNSIGLAAILAIIKKTQEHSAKLSIGGINPKIHIIIQL
                     LDLTNQVEVFSSVEKALEAW"
     gene            7638..8771
                     /locus_tag="PLF66_08615"
     CDS             7638..8771
                     /locus_tag="PLF66_08615"
                     /inference="COORDINATES: protein motif:HMM:NF020084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HMZ64141.1"
                     /translation="MKIEQYIFENQQIDYSQLKENRETQVLFLFGAREIFEKKEIFQK
                     LKGFFPNAIFFGCTTSGEIFKNGVYDLTLTVTALQFEKTKVKFANTKLVEWKDSYTAG
                     KFLANSLDHDNLTHVLVLSDGIHVNGSDLTKGISDHLPTGVGLTGGLSGDAARFEKTF
                     VLVDDTPEEGAISVLGLYGKEIQIGSASMGGWDIFGPERRITKSKGNVLFELDGEPVL
                     TLYKKYLGDAAKDLPASALRFPLNLKIDNLETGVVRTILGMNEEEGSLTFAGDMPEGA
                     YTNLMKANYDRLIDGASGAAEFTSKKNKDKNPELALLISCVGRKLVLDQRVEEEIEAV
                     RDVYGENTIITGFYSYGEISPFTPDAKCSLHNQTMTITTFSEK"
     gene            8774..10291
                     /locus_tag="PLF66_08620"
     CDS             8774..10291
                     /locus_tag="PLF66_08620"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HMZ64142.1"
                     /translation="MDWHSLLKRQIKKHCTDNYNQFIEQNEKFLLAIDEAYNRFDADR
                     LMIERSLELTSQELMEKNKSLLNYIEKLNFIAYHDTLTHLYNRAYLIDKLEEKIKAFH
                     KNPDKTFALLFLEVDRFKIINDAFGHSMGDKLLIKIASRINQFSSENITIARLGGDEF
                     VLLIENYKSEEETLIFANQIISILSPAFKIDYHEVFVTVSIGIAFSNLSYESPADILR
                     DADTAMFYAKKMGRGRVQIFQGSMHNSAAFLIELESNLRQAIDKNEFILYYQPILNAS
                     GTQVNGLEALIRWNHPSKGFISPVEFIPIAEETGMINAIGEWVIRNAVSKCKSLQSLH
                     YGIYISVNLSFVQFKEKNITEIISKILKEEDLDPKLFYIELTESSIMENVFEGIEILN
                     DLRNIGVKLSLDDFGTGYSSLSYLKKFPIHNLKIDQSFVRDIEKGMQDQEIVKAIISV
                     AHSLGLSVTAEGIENRNQLTFLQSCGCDRLQGYFFSKPFPPEEIEEFFNKIKNYY"
     gene            10384..10728
                     /locus_tag="PLF66_08625"
     CDS             10384..10728
                     /locus_tag="PLF66_08625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64143.1"
                     /translation="MAKILSLFLFILFIHCFTDKIQLKGSVEEDRKTLLKHIPIGSSI
                     PEAIRILKQNDFECSIIEKGEFLEERNINFIYCNHYEMVGLFISRRWQIAILLKKEKV
                     DDIHLTTGLLGP"
     gene            10911..13469
                     /locus_tag="PLF66_08630"
     CDS             10911..13469
                     /locus_tag="PLF66_08630"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HMZ64144.1"
                     /translation="MIDLPKPFLLLFILPFLFIFPEEQKIDSSNKKDQIQKEIDELTE
                     KKDKLKKELIEIEEEEKNTKSNASEKKDSKFKEEDFFKLEDNIVVTASRKSQKVSDAP
                     AAVYVITQKTIRERGYRTLVDALHDVPGFDFQHTYGVYPELIHQRGLVGENNRTLVYV
                     DGVPDNNINENAILAGTIRFPLSNVDRIEIVSGPASALYGANAFNGIVNIITKDGKGN
                     PGNHVDFTYGAWEKNFTNPGYSASFSARGSTGSESSDMRYSIGGYYYKTDGPDLSKAN
                     RLDRNGIQNRLGQNDINYYAETKACGGVCNPNSSSVGNWWSDGYSNSKEDTYNLTAKF
                     MKNGLRFQTVNWQYQQGQGAFANGTQQIDTNQRGLDTGKLDGRNIARLLGILRDPQNI
                     GTSGFLGSHRDFKNNSATIGYLHKFSDKLNLDSELVTRSTQVLSSSNEFYPNKTDIYA
                     YYRPNDVVQASGNAIRGASAYSRPDNNYQLEEKLQYNQSEKMTTTVGVVIRQFITPKD
                     YNSYERFVYNTYSGYIQQTYKPQDKVAITAGVRHDFSTTYGNTTNPRLSLIYKPTNDL
                     TLKFLFGSGFREPSAKELFTQTAQRKPNPALRPELMQAHEIGVGYRFLKKYYISTQGY
                     YNQVTNLILEFSTNDTTPINGVNPKNPWQQYQNLGIARIYGFELESNLQISDQFQASF
                     NYTFTKGEYDRLPPTLQTSPATTGRVGDDPRNDLEMAIYRQITVSPQSPNGFNTIPIS
                     GGIPNIAPHKVNAGLTYSPVEDFSIYLGINYVDIRRTVSTDPVRTASGYKMVKLNLRK
                     ENLFYRGLFVQLHINNLLNQQFFDPGIRSATGAYYPTLQPLETRNIWFTVGYQF"
     gene            13542..13922
                     /locus_tag="PLF66_08635"
     CDS             13542..13922
                     /locus_tag="PLF66_08635"
                     /inference="COORDINATES: protein motif:HMM:NF014677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF190 domain-containing protein"
                     /protein_id="HMZ64145.1"
                     /translation="MKIRDKKKELSIYITSKETKGNVPLYKALIDQFISMGITGCTVL
                     KSTSGYGTDLKVKYPDESIADLWSKDSTIVLQVIESASRIEEIVKMLDTMMPQGVVTI
                     KDVDFIRYTRSVVTEEDIRLADKA"
     gene            13972..15777
                     /gene="lepA"
                     /locus_tag="PLF66_08640"
     CDS             13972..15777
                     /gene="lepA"
                     /locus_tag="PLF66_08640"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020764101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HMZ64146.1"
                     /translation="MSDKQKFTRNFSIIAHIDHGKSTLADRLLELTLVTDERTKKNQI
                     LDTMDIERERGITIKANNASFEFLSQDGNKYYLNLIDTPGHVDFNYEVSRSLKACEGV
                     LLIVDASQGVEAQTLANLYLAMEADLKIIPVINKIDLPSADIPKCMRLIEDSLGLESS
                     EAIAISAKTGQNVKEVLESICRLIPPPQGDDNKPLKALIYDSYFDTYMGVVIKVRIMD
                     GTVRRGDRIYLMSGGGDFIVTEVGINRINLIPQDYISAGDVGYIVAGIKKVADARTGD
                     TVTHFDRRAEEAVQGYKEAKPMVFSGLFPIYGEQFDELVEAIEKLKLNDAALVYEREN
                     SLALGFGFRVGYLGLLHMEIVQERLEREFNLELITTAPSVKYRITTTKGEVLEIDNPS
                     KFPEPQSIESMEEPFVKATIFTPNEYVGNIMTLVIEKRGVHLDTIYLDQDKVQMIYEI
                     PLAELIFEFYDKLKSYTKGYASLDYEPAAYRESNLVKLDILVNAEPVDALSTIVHRTR
                     AESRGRAVISKLKELIPRQQFLIPLQASIGARIVARENISAVRKNVTAKCYGGDISRK
                     RKLLEKQKEGKKRMKQFGSVEIPQEAFLAVLKTGD"
     gene            complement(15774..16568)
                     /locus_tag="PLF66_08645"
     CDS             complement(15774..16568)
                     /locus_tag="PLF66_08645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64147.1"
                     /translation="MKNKLTTDTILDGIKEGKAIGMNCFTLTDNFVKNLELVLRTILE
                     NYDRIELLPAIFGTAQELVNWTCLSNMRYIYYKQNDLDLNDEKVFLETEKRFLSSVNK
                     INSANYREYLKSNNMYVHIAFEHEVTGLNIKVHNVSENTLNQEKYLRSYLKQAMHYSD
                     VLEYFNDHPEDPQGKNMGLAFSIIILKESGLRPDLMRISKAGKGSYSRIEIPFVNEYE
                     SIRDKILRDESIVPFERKSLVPPEFQQELERRIKSFDIDSYGFIQN"
     gene            16985..18706
                     /gene="sppA"
                     /locus_tag="PLF66_08650"
     CDS             16985..18706
                     /gene="sppA"
                     /locus_tag="PLF66_08650"
                     /inference="COORDINATES: protein motif:HMM:TIGR00706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HMZ64148.1"
                     /translation="MITTILSILLLPFRLLKSVYFVLFTPFQKGDHILIRIPESFASY
                     QKTSLVEWVTGKDSEILLIDFLNDLELISKSNRIKKVSFYIPSEPEFGMAELSNVTSQ
                     IEKIKAKGIETFGYAASGDIKTLYLLSFLNKRYTSKSSEFMVLLPAVESFFFGNLLKK
                     LGVKVDSFASGPYKSFAEMFNRNSFSIPAKENITMLIRSIKTQILDTFFDNAKLESKI
                     LKRPILTAQYLNEIGFIHGFLEEENFLENYCNKDYEEINEDGSNVLDKEASLRNVHFF
                     HKKKKFEIFPKKKKKLVILPLKGNIGMGKKEEEEIKSDAIQAYSVIPVLRSIRKDKSI
                     IGVILEIDSSGGSAFASELIYQEILKLKKEKKVYAYFQNVAASGGYYIAAACDKIISN
                     PLSITGSIGTIMIRPNLKKLYNEYGVSKQRIEFYPLREIFSEYGQISNEGKEFLTEEV
                     KRVNGQFYERVMLSRNKTLKDLETLAEGRVFTGKSFLASSMVDSNQSLMDTVEELKNE
                     LKEKRILVQYLPSTYSLKSMVREFRLGVRLLLNPKLILKRITRSTDVQLKSDLADVVA
                     ENLFRRN"
     gene            19587..21173
                     /locus_tag="PLF66_08655"
     CDS             19587..21173
                     /locus_tag="PLF66_08655"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002770843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HMZ64149.1"
                     /translation="MDSKKTKNKKETKEKAIEVNLWDAANKLRGSVEPAEYKHVVLGL
                     IFLKFASDKFEEQRAKLISEGKEKYIEKPEFYNMNNVFFLDETSRWSFLISKSKQNNI
                     ALLIDTALHTIEKNNKSLKGALPDNYFSRLGLDTTKLSSLLDTINEIETTSDPKQDVV
                     GKVYEYFLSKFALAEGKGKGEFYTPKSIVNLITEMIEPYKGIIYDPACGSGGMFVQSI
                     KFIEAHNGNKKEVSIYGQEYTNTTYKLAKMNLAIRGISGNLGEKAANTFLNDQHKDLK
                     ADFIMANPPFNQKDWRAENELTDDPRWQGYEVPPTGNANYAWILNMVSKLSENGVAGF
                     ILANGALSGGGTELEIRKKLITNQIVEAILVLPQDMFYTTNISVTLWVLNKNKKAKSK
                     KVGDETRNYRNRENEILFMDLREMGIPFEKKYIQFSEEEIQKIADTYHNWQQENYKKK
                     YKDIPEFCYSATMEEVANKDYSLVPSKYIEFVNRDENIDFDEKMQSLQKEIGELLKEE
                     SKSRKELLNVFKELGYEIKL"
     gene            21157..22296
                     /locus_tag="PLF66_08660"
     CDS             21157..22296
                     /locus_tag="PLF66_08660"
                     /inference="COORDINATES: protein motif:HMM:NF013579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HMZ64150.1"
                     /translation="MKSNYKRIGDFIRLVDERNTGLKVQELIGLTISKQFIPSVANTI
                     GSDMENYKIIRQNQFACSLMQVRRDKKIPVALLKDKEVAIISQAYPIFEIINTKHILP
                     EYLMMWFSRKEFDREACFYAVGGVRGSLEWEDFSNMKLPVPSIEKQKEIVKEYNVILN
                     RIELNNQLIQKLEETAQAIYKQWFVDGVDKDNLPEGWRVGKLRELCEYSNKRIQIEKL
                     NLNNYISTENMIQNRGGITKANNLPNTNNFTNFEIGNILISNIRPYFKKIWLADFNGG
                     CSNDVLCLVPFNNIPSLYLYQILEKDNFFDYVMAGSKGTKMPRGDKKWIMNYPAIIPD
                     KKILEKFNLILTNIQKKIFLMKQEEIKLLELEDFFVSKLATEGRG"
     gene            22381..23301
                     /locus_tag="PLF66_08665"
     CDS             22381..23301
                     /locus_tag="PLF66_08665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64151.1"
                     /translation="MNLFEIEKKEIESVTIFCDERKNINGQWNYLILLIIPTIQLETA
                     LAILTEKRNRIGYNFELKFSDIKKDHGDKFLLASEWIDLIKKDSQKIFHFKILGIDIS
                     KFKNKNFGGDVKKGEYSANMYNRFFRTNLLGLKYFFNPLNIDNIFHDTEGNLENHSYF
                     NWHSIWKVNTTEDNITINSKRIYFINSNHNLEAEYSNYSQLIQLSDIVLGSVSNCFDT
                     NMNNIGQQKLSLKIKDLVERIINNPLNKNSSYNYYKRCDISFFPSEPIHEPESSQAKL
                     EGWIYRKRNLVINSSEIKTKIDNQLKFKME"
     gene            23652..24701
                     /gene="rhuM"
                     /locus_tag="PLF66_08670"
     CDS             23652..24701
                     /gene="rhuM"
                     /locus_tag="PLF66_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RhuM family protein"
                     /protein_id="HMZ64152.1"
                     /translation="MTNSPHNSEILIYQNPEGKIKIDVRLEDETVWLTQAQICELFQK
                     SKSTISEHIKNIFEEEELDEKLVVRNFRTTSRHGAIPEKKQEIEVKGYNLDVIISVGY
                     RVKSTQGTKFRIWATQRLKDHIIKGFTLNEDRFKSGNSMNYFSELQERIREIRLSERF
                     FYQKIKDIYTTSIDYDPKDEKTIEFFKVVQNKLLWAISSQTAAELIYKRVDANLPLLG
                     MQSFDKKSNVSIKKSDVGIAKNFLNEDEIKLLGLLVEQYLAFAEAMAQQKIPMYMKDW
                     ISRLDSILQLNGRELLTHAGRISHEMAIQRSQEEFEKYKQAEREKQKEDSLKELEEDI
                     QKLNKKSIQNESKGK"
     gene            24725..27862
                     /locus_tag="PLF66_08675"
     CDS             24725..27862
                     /locus_tag="PLF66_08675"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HMZ64153.1"
                     /translation="MKFTEEKLEQTFIELLKNEGYSHILGNNVTRKMDEVILEDDLRK
                     YLFTHYLSEGITLNEVDSILLKLKSLPSSDLYESNKLFIQYITDGFILKREDQTQKDI
                     LIHLIDYTEADKNIYKFVNQLEIVGTEKRIPDGILFLNGLPLVVFEFKSAIREDATIH
                     DAYVQLTTRYRRDIPELFKYNAFCVISDGANNKAGSFFAPYEFYYGWRRVAGLAKDVD
                     GINSMFTLIQGMFDRSRLRDIIHNFIFIPDSSKKDEKIVCRYPQYYAARALYENILKA
                     LKPEGNGKGGTYFGATGCGKSFTMLFLTRLLMKSEHLESPTIVLITDRTDLDDQLSTQ
                     FINAKKFIGDNTVESVESRADLREKLQGRKSGGVFLTTIHKFTEDIELLTTRTNVICI
                     SDEAHRSQINLDQKIKITSTGVTKTFGFAKYLHDSLPNATYVGFTGTPIDATLDVFGK
                     VIDAYTMTESVRDEVTVRIVYEGRAAKVILKNSELEKIERYYEEAAELGANEYQIDKS
                     KEETANMYAILGDPKRLQEVAQDFVKHYEKRTSEGSTVNGKAMFVSSNREIAYQLYKN
                     IISIRPEWAEIREADIGVELTEKDKKELKPIEKIKLVMTRNQDDSKELYGLLGTKDFR
                     KELDRQFKNPKSNFKIAIVVDMWLTGFDVPFLDSIYIDKPIQQHNLIQTISRVNRRYA
                     GKNKGLVIDYIGIKKQMNLALAKYNKGDEDNFEDIEQSIVVVKDHLDLLGKLMHTFDN
                     SKYFKGSNLEQLTTLNLAAEFVQQTKDVENRFMGLVKRLKAAYDICVGSEKLSQTQRD
                     YTHFYLAIRSIIIKLTKGNAPDVAQMNARVREMIKDALQSDGVEEIFKLGEEESEQDI
                     FNEDYLAKINKIKLPNTKIKLLQMLLARVIGEIKKINKVKGIDFTKKMQAIVERYNNR
                     NQNDIFKSEVYEEMAEALTNLIWDVHKEFKAGHELGIDFEEKAFYDILKELCIKYDFK
                     YPEDKMIALSKAVKVIVDNQSKFPDWNKRDDIKSALKVELILILDEFGYPPVERDEVY
                     IEIFEQAENFKKNKK"
     gene            complement(28319..28522)
                     /locus_tag="PLF66_08680"
     CDS             complement(28319..28522)
                     /locus_tag="PLF66_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002151096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HMZ64154.1"
                     /translation="MFKVNEGNIDRIIRAILGAGLIIGGFVATGTIAIVLWIAGAVLA
                     GTAATGICLLYIPLGINTCSTKK"
     gene            28731..29735
                     /locus_tag="PLF66_08685"
     CDS             28731..29735
                     /locus_tag="PLF66_08685"
                     /inference="COORDINATES: protein motif:HMM:NF014068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HMZ64155.1"
                     /translation="MFKITFLGLEDSISSFITGPLDLFYHTGRIWNVINGDKEDPFFE
                     VELVSLDGKKITCQNGITLQPKCSIFDSVEQDLIVISSSSFDIGWTLEKYKPVLPYLR
                     SQFEKGIPVASLCIGAFLIAEAGLLNGRKASTHWGFCNLFEEMYPQVKLCREELISED
                     TGVFTSGGANSGLDLTLYLIEKFSGHSQAMNVAKSFVLELGRHGQAPFSKFIGRRSHS
                     DKEMLIAQEWIENNCNRLLSFNEISEKLVMSQRTLNRRFKEATGLSPVDYLQNVRIES
                     AKIFLETGNDTVQEIAYLVGYEDISFFRKVFKKITGITPNQYRQMYRVRNQIQNGFHP
                     "
     gene            29776..30225
                     /locus_tag="PLF66_08690"
     CDS             29776..30225
                     /locus_tag="PLF66_08690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64156.1"
                     /translation="MKRVIAILFLFAIFNCSIPKNHIQSFALENRKDRLETLQKYLIF
                     KSEVLDAEYDIYDVNLNAEYTIPGATSRDYRIVLFLKKEDISKWLTDVTLTSFPESDL
                     WREDLIQKSNRLKSLQLEKDYMTYKNSNKTIFYNQKHSILFIRINQN"
     gene            30347..30619
                     /locus_tag="PLF66_08695"
     CDS             30347..30619
                     /locus_tag="PLF66_08695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64157.1"
                     /translation="MKTVILLLTFLLLTTSSCWDVGYGPYGSEKGYCEYNKYNDSPSS
                     FCLAMVLGSNSESSKKSQALIDACLAVLILEENSCDTKSTIKRYWF"
     gene            complement(30673..32547)
                     /locus_tag="PLF66_08700"
     CDS             complement(30673..32547)
                     /locus_tag="PLF66_08700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HMZ64158.1"
                     /translation="MNLLSIDKISKSAGEKELFRDLTFGLNEGEKVAIIGVNGSGKST
                     FLKIIKGLEEVDGGAVYKNRNLKISSLEQNPSFNPEDTILEHIFKGKSKALSLIQKYE
                     EISERITLESTKELESELHSIMNEIEKANAWEYENQIKSILNELGIKGLNRKMSELSG
                     GMLKKVELVQTLIDESNLLILDEPTNHLDIDTILWLEEYLIKMNKSLLLVTHDRYFLD
                     LVVDKILEISLPQSTLFEGNYNYYLEKKVEMDEANARKEAKAEGFLRVELEWLKRQPK
                     ARGTKQKARTDKIISVINREKFEEQEKFTFKVDPKKVGKKILEIENISKSFGPRNLIQ
                     KFSYTFKHYERLGIVGPNGSGKSTLLNILTGKLNPDSGFVNPGMNTRFGYFDQNSIEL
                     KQSMRVLEYIQKTAGEYIPMENGERISAGLLLERFQFPSKMQSSPVEKLSGGEKRRLY
                     LVQILMHNPNFLILDEPTNDFDIKTLSILEEFLLEFPGCILTVSHDRYFMDRVANHLL
                     VFDGMGNISGFAGTYSEYLSKPKKDLKEKSEPIQEKKQVEDNTNKPQKKKLSYKEEKE
                     LKTIESDIDSLEKEKTQIQSDLTTFHSDFVKVKSLSDRLAEIESLLESKLLRWEELSS
                     "
     gene            32640..33593
                     /locus_tag="PLF66_08705"
     CDS             32640..33593
                     /locus_tag="PLF66_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012390178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HMZ64159.1"
                     /translation="MKKKSKNETLKTISKKAHPAKKKPTYEVSLAIAGGGCKALYGMA
                     VGYKLRKWGVKIKEISGVSAGAAMALGIISETEDESIEYFSELTKRNKSNFQVMSLFQ
                     GKSPFPHENIYRRLIRHGMNLEKVRKSKVKIYIGTVQAIPKKSGLNHYWDIAKLVTET
                     TRAYLLDENDKEEGIPCNRVSTIMERWNMIPIVYTNKDLKSPEVVENIILNSSSIPPV
                     VSFQNNNKVYFLDGGLTNNLLLERFSKKSKKIGVYYEDTTIFGKDRALLESTFLIKPS
                     RKLPIASFDYTDPVAVKECYELGKEDAEKLKPEILNFCRKD"
     gene            33595..34338
                     /locus_tag="PLF66_08710"
     CDS             33595..34338
                     /locus_tag="PLF66_08710"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008591962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HMZ64160.1"
                     /translation="MAKLSVNVNKIATLRNSRGGNIPDLLKYTRVIMDAGAHGITVHP
                     RSDERHITKKDTYDLRKFILEYNKEYNKKIEYNIEGAPDERYIDIILETKPEQATLVP
                     VREGEITSDHGFDLAREGEMLYPIIKKIKSAGIRVSLFLDTNLDNLSYAKQIEADRIE
                     LYTGPFAHAFESGKGVESFMEYEKMAIAALKLGLGINAGHDLDEKNLAIFKKLNGLQE
                     VSIGHRLMSYALLYGLENAVKTYLQALVR"
     gene            complement(34467..36248)
                     /locus_tag="PLF66_08715"
     CDS             complement(34467..36248)
                     /locus_tag="PLF66_08715"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HMZ64161.1"
                     /translation="MFSRIELPQMNLRIKILIGSLILSLVVSVLLAYTSFKILQNELY
                     EEYRDRLIHTSDLGSQLIDQDSLQFLISNLSENLSEEKVNEIQKSKEFLDVYNALNSL
                     RNSKPDLIQYVYIWVSTDNPEEVIYLADADVLRLLEKKAKGETLEDISTYGSRYSISA
                     FPEAKNAFKQNISFVEKDYRYDAQFHTYSISGYSPIKEKKTGKQIAVLGVDMTDTNIR
                     TALKKSTLVSAIIGIITVILTSISSLLLGNLFVKPILELNKVVLKFGEKDFSARANIE
                     TKDEVGLLSDNFNNMAETIVDYDQKLKQLLDSMKRFVPFEFLNFLDKQSIIEISLGDQ
                     VYKEMTIFFSDIRSFTKLSESMTPKETFNFINSYLKRMGPLVRENKGFIDKYIGDSIM
                     AIFPTTPDYALLAGIAIRQELKTYNAQRANNGYAPIHIGVGIHTGNLMLGTIGEVQRM
                     DTTVIADSVNLASRIEGLTKYYGVTTLITETTKSKLINPERFHMRFIGLVQVVGKEES
                     VPIYEVFDGDDEKTFLFKIRITPDFEQGMRYYIEGNLREAHRIFKSIYLENNSDLPSE
                     VYLRKIKHFTKYGLPSDWNGVDKMSSK"
     gene            complement(36525..37466)
                     /locus_tag="PLF66_08720"
     CDS             complement(36525..37466)
                     /locus_tag="PLF66_08720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64162.1"
                     /translation="MEKYISSFFLILQCLLLTACSGCSSTENKKSVTPGLEAEYLVLN
                     EKILMNLYGSPDEKDWAVKTLEKRCEEDGDPHACYNLASHLFAIKNFSGAVRYSERAV
                     IKSPKDALYVEMYRQSLLENGKMEIANKDSLPTNDPSYLITKLELECRNQNKEVAIQI
                     AKDLVGKEVLTAESIRNGFISECLPEKSINELTSVSKKNKVNFASFYYSEKNKSNLFY
                     SIWDTSYFTKNQPIEKEEDLKHQLTMSWREFRKAVQSKNDKLVRKNFHEFLKTLKDEK
                     SQAKQEANLYLAIERAAKLLLEQDEFFAKYRSLADEI"
     gene            complement(37481..39712)
                     /gene="purL"
                     /locus_tag="PLF66_08725"
     CDS             complement(37481..39712)
                     /gene="purL"
                     /locus_tag="PLF66_08725"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF002290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HMZ64163.1"
                     /translation="MEKEKVTLEDAIEHGLKENEFEDICKILGRIPNSTELGIFSGMW
                     SEHCSYKNSILKLKTLPTESSLMLTTTGEENAGALDIGDGLGVVFKIESHNHPTAVEP
                     YQGAATGVGGIMRDIFTMGARPFISLNSLRFGLPDVKRNEYLLKRAVKGIADYGNSLG
                     IAVSGGELFFDSSFSKNPLVNAMTVGVVKKNGMAKATTGGKVGHSVFIVGATTGRDGI
                     HGASFASKDLSKESEEKKSAVQVGDPFMEKLLMEATLEAIEKDILVGIQDMGAAGISC
                     STSEMSAKGKTGMEIDLDKVPFRETGMNAYEAMLSESQERMLVVPKKGKEEELVAIFK
                     KWDLNAVEIGVVTEDGMLRVKKDGKVKAEIPSDTLVLGGGAPRYVREEKRPNYLDEVT
                     KLDISGIQDITAANIGKTLDQMLSNLNICSRKPLYEQYDTDVGLVEVIGPGLDGGLAR
                     VPGTNKGIAVSTDCNSRYTYLDPYKGAIWAVCESARNVFVTGAKPIGITNNLNFANPY
                     IPENYYVFSECVKGIGDACRFLKLPVTGGNVSFYNESPEGPVFPTPTIGMVGLIPDIT
                     HHIKNYFQKPNETIAIVGRFKPTLGGSEFLKEFHNLTKGNIPELSLEDEMNLQNLILS
                     LNQDRLISSAKDISLGGILVALSKMAFPKKVGANIDLSSIKKTRLDETLFGETAASVV
                     ITFPTQNFDQIESICQKNKLEIYNIGKTKATPSIELGELYYEGDLGHLIEKYESRLVS
                     VFE"
     gene            complement(39718..40116)
                     /locus_tag="PLF66_08730"
     CDS             complement(39718..40116)
                     /locus_tag="PLF66_08730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HMZ64164.1"
                     /translation="MSISFPVSPEKQTQLLSRMQKLNIKESDLEETFTKSGGKGGQNV
                     NKVSTAVHLKHKVSGIEIKCSIHRTQGLNRYKARAILCDKLEEKENPKESKAFIQREK
                     IRKTKTKKEKRRQKRLEELKIQDLETEGDF"
     gene            complement(40118..40516)
                     /locus_tag="PLF66_08735"
     CDS             complement(40118..40516)
                     /locus_tag="PLF66_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000451642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog domain-containing protein"
                     /protein_id="HMZ64165.1"
                     /translation="MEEPNIKGMDLVTQHIVMSKDLNSHGNLFGGIMLAWIDEAAALY
                     AMEKIAYTNIVTVNMDDVNFKYPGKNGDIIQIHAAIEKTGKSSLTVRTIALSINEKSR
                     IRNEVINCRVTFVCLDASGKPFPYFEMFGN"
     gene            complement(40522..41115)
                     /gene="nth"
                     /locus_tag="PLF66_08740"
                     /pseudo
     CDS             complement(40522..41115)
                     /gene="nth"
                     /locus_tag="PLF66_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001982194.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
     gene            complement(41229..41516)
                     /gene="rpmB"
                     /locus_tag="PLF66_08745"
     CDS             complement(41229..41516)
                     /gene="rpmB"
                     /locus_tag="PLF66_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HMZ64166.1"
                     /translation="MARKCVVTGRGTAYGNAVSHSHKKTRRIWKVNIVKKNIFLEDEN
                     RWVSVKISTRALRTLKKKGLKSAIKDNGGSLAAIAPKKYVGAKAKTAAKSA"
     gene            complement(42069..42974)
                     /locus_tag="PLF66_08750"
     CDS             complement(42069..42974)
                     /locus_tag="PLF66_08750"
                     /inference="COORDINATES: protein motif:HMM:NF013431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64167.1"
                     /translation="MQKVTLLNASSELELNLDQGAQINKLSLRHPKTGKKIPVVSPFD
                     PNKSFFLSGNFLMFPWVNRLESDKLKIVGKTISIEPLEKDTHSQPIHGLYFNKKRRLI
                     EVRKNSAHIELTEVNPNFPQLSETYTLEDNYLSIQTEIHNPSSQIQEFAYGYHPYLRL
                     NANLDECIIHTNLTHYIPLDGNSLPIKSLEKRDLSYLFTKGEPIGSLKLDHLLTGNFR
                     ELYFGIYDPKDRLLIQVRVPETSQMPMPYFQVYIPEDRKSIAIEPMTSTGNAFFTPKS
                     GLCKIAPGERAFGEFLIELKILPIV"
     gene            complement(42982..44676)
                     /locus_tag="PLF66_08755"
     CDS             complement(42982..44676)
                     /locus_tag="PLF66_08755"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HMZ64168.1"
                     /translation="MKDLRILLIDESDKEEELLKQYLINSDYKPEITKLSDLTQIKET
                     ATKKTFDLIITEHTLSHFNTLDLIKLTQDNNIDIPILIVSGAMNEEIAIEAMKAGAKD
                     YIMKDNMARLIPVIERELRENDSRNARKMAEEAVYYMAFHDPLTGLSNRYEFESRLIK
                     ILEAVKSDSDLQYSLLYIDLDQFKVVNDTCGHPAGDDLLRQVSVILTQSLKKDGFAAR
                     IGGDEFVIILENHSLEQANSFAENLLKNLSEFRFNWMDKIFVIGGSIGIIKISPRVET
                     INEILSMADMACYAAKEAGRNRAHIYIEDDRQMTFRMQEMQWFSRLNESIELDQFTLF
                     QQPIVSVPYQKESSVLFYEFLLRLKTNNNTVIMPDVFMRAAERYNMMPSLDRWVVENA
                     FKYISKNFGDSSSRNRETRFFINLSGASMNDHNFFSFTFRKMKEYGIEPDLICFEITE
                     TVAISDFKKALDFIKEVRKRGSYFALDDFGSGMSSFSYLKNFTVDYVKIDGSFVRDMK
                     DDPLDYAIVESINKIAHIVGLKTIAEFVEDLETMEILEKLGIDYAQGFGIAKPYST"
     gene            complement(44748..45629)
                     /locus_tag="PLF66_08760"
     CDS             complement(44748..45629)
                     /locus_tag="PLF66_08760"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HMZ64169.1"
                     /translation="MPRISVVIPALNEEKYLPILLESLKNQTFKDFEIIVADAGSKDN
                     TIEIAKSYGAKVVPGGMPGPGRNKGAAVATGEFLFFFDSDVLLPTDFLEKAITEMDDQ
                     YIDLATCEFLPLSELTLDKVVFQLSNLFVKINQNLNPRAAGFCIFINRRLFNRIGGFD
                     ESVVIAEDHDLVERASKFRPLKFLKSTSLSVSVRRLDKEGRIALLQKYVKVEMHLLTK
                     GSVKDDIIEYEFGNFDEAEKSKRKKLLNDIEEGIIKLDRQFTKTSNEWNEKTAEVEEK
                     LKSTFDNLNDSIKNFFK"
     gene            complement(45992..46144)
                     /locus_tag="PLF66_08765"
     CDS             complement(45992..46144)
                     /locus_tag="PLF66_08765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64170.1"
                     /translation="MLSSIFSNISLQVSIPRELKPKSQTNDMRIEIYKTLFTPVTDIR
                     KAKKKT"
     gene            46565..47872
                     /locus_tag="PLF66_08770"
     CDS             46565..47872
                     /locus_tag="PLF66_08770"
                     /inference="COORDINATES: protein motif:HMM:NF014803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III PLP-dependent enzyme"
                     /protein_id="HMZ64171.1"
                     /translation="MANKTIKVENEVVKELKEGAEDLDIVKIGVDVPGLVSMINENNI
                     RMYDDEEYTMTDVIKSVIESRASLSSFFLLDIGAIFRKYKLWMRHLPNVKMFYAVKCN
                     SDKLLLHTLVDLGVGFDVASKVEISTVREFDVDPDKLIFANPVKEINHITFAEGEGIK
                     KMTFDNVDELKKISVFHPNAELVLRILVDDSKSRMPFGTKFGCPTSNLPEVFDLAKTL
                     GLNIIGVSFHVGSECESPTAYSDAIALAKEVFNTAKTYGFSLRLLDIGGGFPGHDSPE
                     MENKFIEIAGAINAQLATSFSDVPDLEVIAEPGRFFATSCGTLVTNVIGRKIISNSDS
                     EKIIHYYINSNLYGVFNNIVFDKARPDFELRTPVGENEPLYKSVIFGQTCDSMDKIAD
                     GIMLPELVCGNWLIIRNHGAYTIASASKFNGFELEDVNYVFTF"
     gene            complement(48112..>49774)
                     /locus_tag="PLF66_08775"
     CDS             complement(48112..>49774)
                     /locus_tag="PLF66_08775"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HMZ64172.1"
                     /translation="LSAGKVGTSPENSIPDYKNTQAFLSNPEEDVEIIYWISNYEHRN
                     GGAWYSLILGTHEQIISETNFFRDMDFFLTGIIFIMILYHLFLYFYRRSDISNILFSV
                     FCFVILLRSLSIGERVFNSYIQVPFGLLIRVEYFTWYATLPLGYHFLYSQFNRPYLKR
                     LTIICYCISILLSIGLFLPPYFFTNSVPLYQPFYFGFILLGFSFMIYYVYQNLPGAKL
                     ILFGFVFMILTTVNDLLYANQWINSFFLSHYGLACLIFSQSLGISIRFSKAFRSIENL
                     TTDLKEVNKAYSRFVPIEFLNFLDKQNITEIRLGEQVQRDMSILFSDIRSFTELSEKM
                     SPSDNFNFLNSYLSRMGPIVRSHNGFIDKYIGDAIMALFPGNVEDALNAAIEMQEKIR
                     IYNQHRMNYNYDSIRVGIGVHTGPMILGTIGEEERLETTVISDAVNLASRIEGLTKVY
                     GAYILISEYTFKNLKNPEKYKYRILDRVKVKGKSNTIAVYEIFNGMPDYLLDLHLKTK
                     DDFEKGLDYFWNKDFQKAIECFSRVISIFPADQASVHFLSLSQDQ"
BASE COUNT        16991 a         7599 c         8855 g        16329 t
ORIGIN      
        1 gaaaataaat tctgactgct tgagaaaaag ttttcctatt catttgttgg aactataaag
       61 aaagaagcag tgttagttaa caaagctttc aatattccgg aggatagtta gttcaactga
      121 aatcaaatta acgatagccg ctagaaagtt aaaatttagt tttctaatta aaaaaagatt
      181 ggaaatagtt cctagtcata ctaaaaaagt taataattca tttattaata tgcgtaaatt
      241 gattcgaaat cttatatttt taaacttttc cgttcttatc attttatttt caaatttttc
      301 tttgtattct gactcagtct tcgcggatgg agcctagacc taaaagataa ctgggatata
      361 gaaaaagggg aaattatttc tttaaaaggg aattgggaat tttttcctga ggaatttgta
      421 ttagactctt cacgaaaatc taacattttc attccttttc cctctggttg ggatgaaatt
      481 ccaagttcta aaatgaaaaa tcaatttgga tatgcaacct atcgcttaaa gataattctt
      541 cctgagaatt ccaaaaacca tcaactttct ttgagattgg ttaatttact cacagcgtcc
      601 aacgtatatg tgaatgatat acttatttat aagagtggca aaatttcaaa aacacctgaa
      661 aattcagagg cacaaatact tccagcatcg gtaaatttac ctcagctaga aaaagaattt
      721 ttattaacaa ttcagatttc caattttcat caaaacaaag gcgggattta ctctgcaccg
      781 gaactaggtt ctcagttagc aatctcaggt cgaagggaaa agcaagtcgc aattgatatg
      841 ttactctttg gctccattat tattatgggg atttatcata tcgtattatt agtgattagg
      901 agaaaggata catcaaattt attttttgga atattttgta ttttgctaag cacaagaatt
      961 gctattatgg gggaaaggat ttttatccat tggtttccga agccgggtta cgatttttat
     1021 atgcgattgg aattaattag ctactttctt gccattccct catttacttt gtatgtaaaa
     1081 agcttgtatc caaatttaat aggtaaatgg attgcagttg tgtctatttt ctttggtgta
     1141 ttatattctt taataagtat actaacgcat gcatctatat ttacgaattt gatttttcag
     1201 tcttccttaa ttctgatttt tattggtatt tatattataa ttgcaatcac tagggcatgg
     1261 tttaaaaagt tacagggagc caaaagtttt ttatttggat tctttatttt gtttttgagt
     1321 tcgcttaacg atatagtaaa ttcgttaaca aatgaattta atacagggat ttacatcgtt
     1381 cctgttggtc ttttgatttt tattttctca cagtcctatc ttttatcggt tcgttattca
     1441 aatgcatttc agaaaattga agaattatct tctaaccttt tcgaattaaa tactactttg
     1501 gagcaaaagg taatcaatcg aacaaaagaa ttacattcaa cattaacaga agtgcaaaac
     1561 ctaaaagaaa agcaagacgc agactatttc ttaacgtctc ttctcatcga cccattaaaa
     1621 acccatgata taacaggtga taaagtcaaa attgaattta aaatacaaga aaagaaacaa
     1681 tttacatatc agaataatac gaaaggaatt ggaggagact tatgcctagc taaaacaatt
     1741 attttaaaaa acagatctta tagtctattt gtaaatgcgg atgcaatggg caaatctctt
     1801 atgggtgctg gtggagttat tgtattaggg tcggtaatga aagcatgtat cgaaagaaca
     1861 attcaaactc ccactgaaaa attattatat ccggaacgtt ggttaaaaaa tattttctta
     1921 gagcttcata aaatctttga atcttttaat ggctatatgt taacttcgat tgtgattggt
     1981 cttgttgatg agaaaacagg tttagtgtat tttataaatg cagaacatcc ttatgtaatt
     2041 ttatatagat cccaaaaagc aaaatttata gaaagccaaa tccatttttc gaaacttggg
     2101 atcgggaatg aagattctac aatttggatt cagacctttc aaatgaagcc aaatgatgta
     2161 atactagttg gctcagatgg aaaagatgaa ttattgaaat atgataaaca tggtaatact
     2221 tctattgttt ctgattataa gtatttttta aaaatagtag aagaggcgaa tggaaatatt
     2281 gatagtattt tcgaaatttt gaattcgaag ctagaacaat taatagatga cctttctttg
     2341 cttcgtttat cttatttaga aaataataaa cctcaagtgc aaactacaat tatagataaa
     2401 ataaaagatg atatttctaa aggcataatt ctttttaaaa ataaagacta tataaatgca
     2461 ttgaacgttt ttcaagatat tttgaagcat cagccagaaa atgagattgt gatccgttat
     2521 gcgaatgcct gtgcaataaa aatggaagat tttaaattag cattggagta tggattacag
     2581 ttaatcgaaa ttcgacctga taaaagcttt cctatatttc aaacatcgat cctagcacgt
     2641 aggaataatc agctattact tgcagctgat ttaggagaaa gatatagact tagaaatcca
     2701 aagaatataa aaaatctgtt ttcattgatc gaaactcatt ccaagatggg gaatcattct
     2761 agaatcgaaa aactcttaaa agaaataaga actataaatc caaatcatcc actattacat
     2821 aaattttaaa gatttatact ctccttcatt gaatgaatct cacctcaaat catctggagt
     2881 tggaaaatat cccttatttc ttttcagaca ttttattctg aaatcacttt gaaaatttca
     2941 cacaagattt ttaattctct ctaacaccga taaagaggct ggggaaacgt gagtgcttat
     3001 tcaaatacat aaagaaaagt cccatttgaa aaaaatgcga taggtctaac actactcaac
     3061 atttccccaa ttggatctgt gcggtttagt aagtgcaatc gagtaaatta atttataaaa
     3121 ttaccttcct ttcgctgtca tcggtcttaa tcgttgatag atttggagaa agctatcaag
     3181 ataaaaaggg gcttagcccc ttattagttt ctaatgcatc taagattttt taaggttgtt
     3241 ttttgcgctt ggtaaatatt tgtttggaat ggacctgtat ttgaaacctc attcgcaaat
     3301 cctgataaca cagattgatt tgaccaataa tttaacccta aagagctagg ccaaccattt
     3361 ggattttttg catagatagg aaagaatcca gttaattcat tgagtgtggg agctctccaa
     3421 gttccagcgg caaatccacc gtatgttcta atattgcaat agttaaagat aggacttcca
     3481 ttcgctgctt gtcctgtgtc agttccaccg ttacaactat tgtcattcgt tgcacaaaat
     3541 tgaaaatctg ttggtgttcc agaacaattt cctgtagaag tattataagt ttctccttca
     3601 ctgcaaatat aaaccttttt attcattata tttgtatttg cagaggcatc tactatggtt
     3661 aaataagagt tatccgctga ccgaatcgca gaaactttaa aggtttttga atcgatatca
     3721 gaaggcgtat aatttgctct acatcctatc gcacttacag tgacatctgc atttgtatta
     3781 taagaaagtg tggtagaaga accagttgca cagcttccat ccgagttaca agcaggagtt
     3841 ccattggctc tgaggcattt agtaatgctt ggtacagaag aagtggtggc tgaaaaagaa
     3901 aacggtatta cgagcaagtt aaatgtattg ttggaataaa caagtgtttg tgctactttt
     3961 aaagtatagg tagcggttga cacatttgaa tctgctagtc caactttaca agcaattgct
     4021 ttaacgatag ttcctgtaat atcaatcaga atgggtgaag tataaagagt tccatttcca
     4081 gaacaatttg gatccgttgg atttaaccca cgattaaaat agatatttgc gtctaacgtt
     4141 gcactcgata gactgatact ttggtctttt gaataaattc cagttgctac gttaaaggag
     4201 ggtgcgctca cttgaccttg aatggtaaca agaacttggt attcttttgt tgaactgtct
     4261 tcagcaatca cggtatatac gattcctccg ctaaaatcat ttgcagtcac tcctgaaatt
     4321 tgatctatat taccgatctt aaccgatttt ccattggatg taaaagtagc aactagacta
     4381 gatagattgg ttccgagggg aacttctaga ttaatcacat tgctagaaat ggaagaggtt
     4441 gctgcaattc ccaaaaaaga ataggaagta atgtcttttg tgtttgattg aggagtggaa
     4501 gaatttacag ttccattcga aatgaattgg gaataggaat ttccatttac aagtccgaat
     4561 aggagtccgg aaggactatt gatatcaaaa cgagattttt ttgcttgtag gcatccaaat
     4621 aggaaaagga ctaaaatgat aaagtaagtt ttcgtaatta gaaatgtatt ttgttttgtg
     4681 ctaagttgtt tcattgtttt tatttttgat tctagtttgt taaatattat taatctattc
     4741 taatgataat aacagggcag acaaattaga tttgcaaaat ttaaaaaact tttagtaaaa
     4801 aatatattga ctaggaaacc ttctttttgc tgcaagtgta atcacttgga aaaaggattc
     4861 ccgtttgttt taaaaatctc tctattggaa agaatcgaga tttatttctt ctcttttaaa
     4921 ctttcaaatt cttgtttcag gtgaacactg tggaagatgt tcaatgaaag cgagccgata
     4981 ggtgttaggt agacaaaact aaatcaatag aacgtttttc tgagatttgt aattttctaa
     5041 aatcatttta agaggataga tataaaaaaa taatcacact aataatatta acaatgattc
     5101 gaatcagatg cacaggggta gacacggaac acgatccgaa atgctcgtga aggatgggca
     5161 ttggataagg aaacatgtaa tgcactacta cccacgtttt atggcaaaac ggatcctgat
     5221 cttcaaagga tatacaatgg catgctagcg agttgtctgt tgtataacgt agaaaagtat
     5281 caatgtgaaa aaaaactaat gtttttccct tcatcggaaa aagtttataa aatggaggga
     5341 ttcgctggat acaggataag gtcaaaaaaa gccaacatca gaactcatcc tgaatctata
     5401 gtggttgcga ctctcgatca aagagatcga aaatgacact gtcgcggttc caagctagta
     5461 aattgggaaa aagtgtctca ttgtttttat aaaaccacca ttgaatcaaa tggaactcac
     5521 gttgacttaa ttgaaactct ataaactctg tccttagaag tatatagata tgaaacaaat
     5581 taacattgga ctcattggag ctggaacagt aggctctgga ttaatagaaa tattacactc
     5641 tcatagaaag caaatcttag atagaacggg tattgatcta aatctagtaa aaatatgtga
     5701 tacggacaca tctcgaattc cttcctattt caaaggggaa gctgttaccg attttaaaaa
     5761 aataacagag gaccctaaaa tccaagttgt agttgaatta gtcggtggaa cagggattgc
     5821 ctacgaaatt gtgaaatcag ctctttcgaa tgggaaagca gttgtgactg caaacaaagc
     5881 attgctctcc gaaaaaggac ctgagttatt ccaattagcg gaagaaaaaa aagtagaaat
     5941 aggatatgag gcaagtgtcg ggggggcaat ccctgttatt cgctctatca agacaggtct
     6001 tgtagccaat gattttcaat ctatctttgg gattttaaat ggaactacaa attatattct
     6061 cactaaaatg gaagaggata atatcggtta tgcggatgct cttaaacaag cacaagactt
     6121 gggatttgcg gaaaaagatc ctactttcga tgtggaagga attgatgcag cgcataaact
     6181 ttctatttta atcggcatag catttaataa aaaaataaaa atcagtgata ttcatataga
     6241 aggaatttca aaattatctg aggcagaaat ccatgctgca aaactacttg gtcttagaat
     6301 taaattatta gctgtatgta aacaagtagg ggatcattta gaagcgagag ttgaacctac
     6361 aatgattcca ctaacgcatc caattgcgag tgttcgaaat gaaatgaatg caattttttt
     6421 cgaaactagt ttttctggtc caagtatgct acttggtaag ggagcaggtt ctcttcctac
     6481 tgcttctgcc atcatatccg atcttgtatt ttatggatct aggttaaatg agttgggaga
     6541 aataaaagaa aaaaatctat ttgaagaagg aaaactgaca cctccaggag agataaaagc
     6601 tagatactat ttaagattta aaacagttga tagacctggc gttttgggag agttaacgag
     6661 tataattgga agaaatttag tttccatttc ctctatgcga caaaacgaat caaattcaga
     6721 acctgttgaa ctgattatca ttacacatga agcgttagaa aaagatgtta gaaattcact
     6781 tatggagatt gataagcgtt cggatatcat taaaggcaaa tcgattatga ttcgcctaga
     6841 ggatctaatt tgaatcgatg cacgatatca gagcatagat tagaaaaaga gaaacatcta
     6901 gatatttttt tcgaaactga gccacattta gagacgtatg aagcagaagt ggatcagttt
     6961 tctttacttt tgtcacaaaa aaaaattttt tgtagaaaga aacaagcgca cagaaaaatc
     7021 tatttagagt atgaaggtga agtatcagca aatagaggaa atataaagat tttgtggaaa
     7081 ggtttttatg aaaatagtct atccttcttc ttagaagaga ttgacttatt tttattaaag
     7141 gaaaatcttt tacggataga tgcaagatct tgattgatgg aaatggaaat gagagaaatt
     7201 aatttcgatg cattagttgt aaaagaaaga attgaagtaa tcaatgatac taaggttcag
     7261 attttatatt ttgttggtca aatcgaccaa aataattcct acacaatttc tgataatata
     7321 aatgcttttt ttgaatcagg aactttcaat acaatactag atctttccaa tttagtttat
     7381 gttaatagca ttggtcttgc tgcaatactt gctattataa aaaagaccca agaacactcc
     7441 gctaaattat ctattggcgg aattaatcca aaaattcata ttatcatcca attactagat
     7501 ttaacaaacc aagtagaagt tttttcttct gttgaaaaag cattggaagc ttggtaaaaa
     7561 ataaaaaaaa cttctagtaa attattctaa attcataaac ctgttgtttt aaagaatgaa
     7621 cataggttac cttttagatg aagatagaac aatatatttt tgaaaaccaa caaatcgact
     7681 actcccaatt aaaagaaaat agagaaacac aagtcttatt tttattcgga gcaagggaaa
     7741 tctttgagaa aaaggaaatt tttcagaaac taaaaggttt ttttccaaat gctattttct
     7801 ttgggtgcac aacttctggt gagatattca aaaatggagt ctatgattta acgttaactg
     7861 ttactgctct tcagtttgaa aaaactaaag taaaatttgc aaatactaaa ctagttgagt
     7921 ggaaagatag ttatacagcg ggtaagtttt tagcaaattc attggatcat gataatttga
     7981 cacatgttct tgtcttgtct gatggaattc atgtaaatgg tagtgatctt acaaaaggaa
     8041 tcagtgatca tttaccgacg ggtgtaggtt taacaggagg tttgtctgga gacgccgccc
     8101 gttttgaaaa aacatttgtt cttgtggatg atacaccaga agaaggagca atcagtgttt
     8161 tgggtcttta tggaaaagag attcaaatcg ggtctgcttc tatgggagga tgggatattt
     8221 ttggaccaga aagaagaatt acgaaatcaa aaggtaatgt tcttttcgaa ttagatggag
     8281 agccagtatt aaccctttat aaaaaatatc taggtgatgc agcaaaagat ttaccagcct
     8341 cagcccttcg attcccgctc aatttaaaaa ttgataattt agaaacaggt gtagttagaa
     8401 cgattttagg tatgaatgaa gaggaaggta gtctaacgtt tgcaggcgat atgcctgagg
     8461 gagcgtatac gaatttaatg aaggcgaatt atgatcggtt aattgatgga gcttctggtg
     8521 cggcagaatt taccagcaag aaaaataaag ataagaatcc agaacttgca ttactcatta
     8581 gctgtgttgg tcgaaagctt gttttagacc agagagtcga agaagaaatt gaggcagtta
     8641 gagatgtata tggagagaat actattataa caggctttta ctcttacgga gaaatttctc
     8701 cttttactcc tgatgctaaa tgttctttgc ataaccaaac tatgacgatt actacattct
     8761 cagaaaaata aaaatggatt ggcatagttt acttaagagg cagatcaaaa agcactgcac
     8821 tgataattat aatcaattta tagagcagaa tgaaaaattt ctgttagcta tagatgaggc
     8881 ttataatcga tttgatgcag atcgtttaat gattgaaaga tctctagagt taacgtcaca
     8941 ggagttgatg gagaaaaata aatctcttct aaactacatt gaaaaattaa attttattgc
     9001 ctatcatgat acattgactc atctctataa ccgtgcttat ttaatagaca agctagaaga
     9061 gaagattaag gcctttcata aaaatccaga taagacattt gctttattgt ttttagaagt
     9121 agatcgtttt aaaattatca atgatgcttt tgggcatagt atgggcgata agctcttaat
     9181 taaaattgcc tctagaatca atcagttttc atctgagaat ataacaatcg ctcgacttgg
     9241 tggagatgag tttgttttat taatcgaaaa ttataaatct gaggaagaaa ccttaatatt
     9301 tgcaaatcaa atcatttcta tattaagtcc cgcttttaaa atagactatc atgaagtatt
     9361 tgtaactgta agtattggaa ttgctttttc aaatttatct tatgaatctc ccgctgatat
     9421 tttgcgagat gcagatacag ctatgtttta tgctaagaaa atgggaaggg gtagggttca
     9481 aatttttcaa ggttcgatgc ataattcggc tgcttttttg attgaattag aatccaattt
     9541 aaggcaggca attgataaaa atgagtttat actttactat caacctattt taaatgcgtc
     9601 aggcactcaa gttaatggtc ttgaagcttt aattcgatgg aatcatccaa gtaaaggatt
     9661 tatttctcct gttgaattta tcccaattgc agaagaaaca gggatgatta atgcgatagg
     9721 agaatgggtt atacgaaatg cagtatctaa atgcaaatca ctccaatccc tgcattatgg
     9781 gatttatatc tctgtaaatt tatcttttgt tcaatttaag gaaaaaaata ttacagaaat
     9841 tatttctaag atattaaaag aagaagattt agatcccaag ttattttata ttgaattaac
     9901 agaaagtagt atcatggaga atgtttttga gggaattgaa attttaaacg atctaaggaa
     9961 cataggtgtt aaactttcac tagatgattt cggcacagga tattcttcgt taagctattt
    10021 aaaaaaattc cctattcaca atttgaaaat tgatcaaagc tttgtgagag atattgaaaa
    10081 gggaatgcaa gaccaagaaa tagtaaaagc aattatctct gtagcgcata gtcttggact
    10141 cagtgtaacg gcagaaggaa tcgaaaatcg aaatcaactt acatttttac aatcctgtgg
    10201 ttgtgatcgg ctgcaagggt atttttttag taaaccattt ccacctgaag agattgaaga
    10261 attttttaat aagataaaaa actattactg aactttaccg acaatacgat agatcctagt
    10321 aattgattgc gttttctagt ctttctaaaa gaaaataaaa ctcttaattt aagttaatga
    10381 actatggcaa aaattttatc cctatttcta ttcattcttt ttattcattg ttttactgat
    10441 aaaattcaat taaaaggttc agtagaagag gatcgcaaga ctctattaaa acacattccg
    10501 attggaagtt ctattcctga ggcaattcgc atattaaagc aaaatgattt tgagtgttct
    10561 attatagaaa aaggagaatt tttagaagag agaaatataa attttatcta ttgtaatcat
    10621 tatgaaatgg ttggcttatt tatatcaaga cgttggcaga ttgctattct tttaaagaag
    10681 gaaaaggtgg acgatattca cctaacaact ggtttacttg gaccctaaat tgtatttcta
    10741 tagattaaac taatttcgtt ttaatggatc aggattcctt cttgacttta ttttcctatg
    10801 agtctaattt ttaggattta tttataaaga ctgattgata agaattttga tttgaaagtt
    10861 gtaagttcat tcaaagactt tttttgagtg aactatagaa aggatttagc atgatcgatt
    10921 taccaaagcc attcctacta ttatttattt tgccattttt gtttatattc ccagaagaac
    10981 aaaaaataga ttcttcaaat aaaaaagatc agatacaaaa agaaatagat gagttaacag
    11041 agaaaaagga caaattaaaa aaagagctaa tagagattga ggaagaggaa aaaaatacaa
    11101 aaagcaatgc ttcagagaaa aaagattcta agtttaaaga ggaagacttt ttcaaattag
    11161 aagataatat tgtagtaact gctagtagaa agtcccagaa agtctcagat gctccagcag
    11221 ctgtgtatgt gattactcaa aaaacaattc gagaaagagg gtatagaacg ttagtagatg
    11281 cattacatga tgtccctggt tttgattttc aacacacata tggagtttat ccagaattga
    11341 ttcatcaaag agggcttgtt ggtgaaaaca atagaaccct tgtttatgta gatggagttc
    11401 ctgataataa tataaatgaa aacgcaatat tagctggcac gattcgattt cctctttcga
    11461 atgtagatcg aatcgaaatt gtctccggtc cagcttcggc tttgtatgga gccaatgctt
    11521 ttaatggaat tgtgaatatt attacaaaag atggaaaagg gaatcctgga aatcatgtag
    11581 attttacata tggagcctgg gaaaagaatt ttacaaatcc tggttattca gctagttttt
    11641 cggcgcgtgg tagcacagga tctgaatcta gcgatatgcg atatagtata ggcggatatt
    11701 attataaaac agatggacca gatttaagta aggcaaatcg cctagatcgg aatggaatac
    11761 aaaatcgatt ggggcaaaac gatattaact actatgcaga aacaaaagcc tgcggtggtg
    11821 tttgcaatcc aaattctagt tctgttggaa attggtggtc agatggatat agcaattcga
    11881 aagaagacac atacaattta actgctaaat ttatgaaaaa tggtcttaga tttcaaacag
    11941 taaattggca ataccaacaa ggacagggag catttgcaaa tgggacccaa caaattgata
    12001 caaatcaaag aggacttgat acgggaaaat tagacggtag aaatattgca agactacttg
    12061 gaatcttacg cgatccacaa aatattggaa cgagtggttt tttaggatcc cacagggatt
    12121 ttaaaaataa tagtgcaacc attggatact tgcataaatt ttccgataaa ttaaatctag
    12181 atagtgaact tgtgacaagg tcaacacagg tgctaagttc gagtaacgag ttttatccaa
    12241 ataagacaga tatctatgcc tactatcgtc ctaatgatgt tgtgcaggca tctggaaatg
    12301 caattcgtgg agcgtctgct tattccagac cggacaataa ttatcaatta gaagagaaac
    12361 ttcaatacaa tcagagtgaa aaaatgacaa ctacagtcgg tgttgtaatc aggcaattta
    12421 taactccaaa ggattacaat agttacgaaa gatttgttta taatacttat tcagggtata
    12481 tacaacagac ttataaacca caagacaagg ttgcgatcac agctggagta agacatgatt
    12541 tttcaactac ttatggaaat acaaccaatc ctagattgag tttaatttat aagccgacga
    12601 atgatttaac tttgaaattt ttatttggta gtggttttag agaaccatct gcaaaagaat
    12661 tatttacaca aactgctcaa agaaagccta acccagcact tcgtcctgaa ctcatgcaag
    12721 ctcatgaaat cggagtcgga taccgttttc ttaaaaagta ttatatttca acgcagggtt
    12781 actataacca agtgaccaac ttgattttag agttttcaac gaatgatacc actcctatca
    12841 atggagttaa tcccaaaaat ccatggcaac aatatcaaaa tttaggtatt gctcgaattt
    12901 atggatttga attagaaagt aatcttcaaa tttcagatca atttcaagca tcctttaatt
    12961 atacatttac aaaaggagaa tatgatagat tgcctccaac actacaaact tcaccagcta
    13021 caacaggaag agtgggggac gatcctagaa atgatttgga aatggcaata tacaggcaaa
    13081 tcaccgttag cccacagtct cccaatggat tcaatacaat tcctatttcg ggaggaattc
    13141 caaacatagc tcctcataaa gtgaatgcgg ggcttactta ttctccagtc gaagactttt
    13201 caatttatct gggaattaat tatgtagaca tccgaagaac tgtttcgact gatccagttc
    13261 gaactgcgag tggatataaa atggtaaaat taaacttacg aaaagaaaat ttattttaca
    13321 gagggctatt tgttcaattg catattaata atttattgaa tcagcaattt tttgatccag
    13381 gtatccgatc tgcaacagga gcgtattatc ctactttgca acctctcgaa acaagaaata
    13441 tttggtttac cgtcggctac cagttttagc tgaaaaagaa tattttatag tttacataca
    13501 aaacactggt aatattatct gaaaatcaag aagaggcgac tttgaaaatt agagacaaaa
    13561 aaaaagaact ttccatctat ataacaagta aagaaacaaa agggaatgtt cccctatata
    13621 aagcgctgat tgatcaattt atttccatgg gaattactgg ctgcacagtt ttaaaatcta
    13681 catccggata tggaaccgat ttaaaagtca aatatcctga tgaatccatt gcagacttat
    13741 ggagcaagga ttctacgatt gttttacagg tcatagaatc agcttctaga atcgaagaaa
    13801 ttgtaaaaat gttagataca atgatgccgc aaggagtcgt tacaatcaag gacgtagatt
    13861 ttattcgata cacaagaagc gttgtcacag aagaggatat tcgccttgcc gacaaagcct
    13921 aatacaaaat gacttgctaa atagcgcacc tctgtaaatt taatttacag aatgtctgac
    13981 aaacaaaaat ttacacgaaa tttctcaatc atagcacata ttgatcatgg caagtctact
    14041 cttgcagatc gtttactcga attaactctg gtaacagatg agcgcactaa aaaaaatcaa
    14101 atcctagata caatggatat tgaaagagaa agaggaatta caatcaaggc aaacaatgct
    14161 tcttttgaat tcctttctca agatggaaat aaatactatt taaatttaat tgatacaccc
    14221 gggcatgtgg attttaacta tgaagtttct agatccttaa aagcatgtga gggagttttg
    14281 ctgattgtag acgcaagcca aggggtagag gcacagactc tagccaattt gtatttagca
    14341 atggaagcag atttaaaaat cattccagtg attaataaaa tcgacttacc aagtgcagat
    14401 attcctaaat gtatgcggct aatcgaagat agcctcggtt tggaatcatc agaggcaatt
    14461 gcaatttctg caaagacagg acagaatgta aaagaagtat tagaatctat ttgtagactc
    14521 attcctccac cacaaggaga cgataacaaa ccactcaaag cattgattta tgattcttat
    14581 tttgatacct atatgggtgt agtcatcaag gttcgaatta tggatggaac cgttaggcga
    14641 ggggatcgca tttatttaat gagtggtggt ggcgatttta tcgtgacaga ggtggggata
    14701 aatcgtatta acctcattcc acaggattat atatctgcgg gagatgtagg ttatattgta
    14761 gctggaatta aaaaagtagc agatgcaaga acaggtgata cagtcactca tttcgataga
    14821 agagcagagg aggctgtcca aggctataaa gaagcaaagc caatggtatt ctccggactc
    14881 tttccaattt atggggaaca gtttgatgaa ttagtcgaag cgattgaaaa attaaagtta
    14941 aacgatgcag ctcttgtata tgaaagagaa aattcgctcg cactcggttt tggatttaga
    15001 gttgggtatt taggtcttct ccatatggaa attgtgcaag aaagactaga gagagaattc
    15061 aacttagagc tcatcacaac agctccttct gtaaaatata gaattactac aacgaaagga
    15121 gaagttttag aaatagacaa tccttctaaa tttccggaac cacaatcaat tgaaagtatg
    15181 gaagaaccat ttgttaaggc aacgatcttt actccaaatg aatatgttgg aaatataatg
    15241 actctagtga ttgaaaagag aggagttcat ctagatacaa tttatttgga tcaagataag
    15301 gtgcagatga tttatgaaat tcctcttgct gagcttattt ttgaattcta tgataaatta
    15361 aaatcataca caaaaggata tgcatcttta gattatgagc cggctgccta tagagaatcg
    15421 aacttagtaa aacttgatat cttagtaaat gctgaaccag tagatgctct atctactatc
    15481 gttcatagaa ctcgcgcaga atcaagaggt cgggctgtaa ttagtaagtt gaaagaacta
    15541 attcctagac aacaattttt aattccactc caagccagta tcggagcaag gattgtggcg
    15601 agagaaaata tctctgcggt tcgtaagaac gtaactgcta agtgttacgg gggagatatt
    15661 tctagaaaga gaaaactcct agagaaacaa aaggaaggaa agaagagaat gaaacagttt
    15721 ggttctgtgg agattccaca agaagcattt ctagcagttc tcaaaacagg agattaattc
    15781 tgaataaatc catacgaatc aatatcgaaa gatttgattc gtcgctctag ttcttgctga
    15841 aattcaggag gaactagact ctttcgttca aatggaacga tagattcatc tcgtaaaatt
    15901 ttatctcgaa tactttcata ttcatttaca aacgggattt caatacgaga ataggagcct
    15961 tttcccgctt tgctgatacg cattaaatca ggtctaagac cagattcttt tagaatgata
    16021 atagagaaag caagccccat gtttttgcct tgagggtctt ccggatgatc attgaaatac
    16081 tctaaaacat ctgaataatg cattgcttgc tttaaataag agcgaagata tttttcttga
    16141 tttagagtgt tttctgaaac gttatgcact ttgatattga gtcctgtaac ttcgtgttcg
    16201 aaggcaatgt gaacatacat attattcgat ttaagatatt ctctataatt ggcagaattg
    16261 attttattta cactggatag aaaacgcttt tcggtttcta aaaaaacttt ctcatcgttt
    16321 aggtctagat cattctgctt atagtaaatg tatcgcatat tcgaaagaca agtccagttt
    16381 acaagttctt gcgcggttcc gaagattgca ggcaaaagtt ctattctatc ataattttct
    16441 aatatggttc ttaggactag ttctaggttt ttaacaaaat tatctgttag agtaaagcaa
    16501 ttcattccaa tcgctttacc ttccttgatt ccatctaaaa tcgtatcagt tgttaatttg
    16561 tttttcatac atacctagcc caatagtgta agtatccatt taaagccaaa atcattcaat
    16621 aaattttttt tgttccgata gatattgaag gttgtagtgc aaagatctct tttttaaaca
    16681 tgatgcacag ctatggattt aatatttatg aatattcctt actatttgaa tgagttgtgt
    16741 ttatttaaac caatttggct tctcaatgat tgtaacagaa tatggtaatc tttcattcat
    16801 atatgaatta tagcatttgg tagaccgaac acacttaagt tcgggaacac taagaagtgt
    16861 ttagactaaa cccaaccatt tatttttgaa acgggacttt ttcttgacgt ttagtataag
    16921 tatggaaggt agaataaata aaggctttaa aatctaatag gcttttaaat tctaacttac
    16981 aagcatgata actacaatac tttctatttt acttcttcct tttaggcttt tgaaatctgt
    17041 ttattttgtt ttatttacac cctttcaaaa aggagatcat atactaattc ggattccaga
    17101 atcctttgcc tcctatcaaa agacctctct tgtggagtgg gtaacaggta aagattccga
    17161 gattcttctc atcgattttt taaatgatct agaattaatc tcgaagagca atcgaattaa
    17221 aaaagtttct ttttacattc cctctgaacc agagtttgga atggctgaat tatcaaatgt
    17281 tacctctcag atagagaaaa tcaaagcaaa gggaattgaa acctttggtt atgcggcaag
    17341 tggagatata aaaactcttt atcttctttc ttttctaaat aaaagatata cttcaaaatc
    17401 aagtgaattc atggttttac ttccagcagt agagagtttc ttttttggaa acttgctaaa
    17461 aaagttaggc gtgaaagttg atagttttgc ttccggtcct tacaaatcct ttgcagaaat
    17521 gttcaatcga aattcttttt caatacctgc aaaagaaaat ataacaatgc ttatccgctc
    17581 aataaaaaca caaatcctag atacattttt tgataatgca aaactagaaa gtaaaatctt
    17641 gaagcgtccg atcttaacag ctcagtatct aaatgaaatt ggttttattc atggattttt
    17701 agaagaggaa aatttcttag agaattattg caacaaagac tatgaagaaa taaatgaaga
    17761 tggaagtaac gttctagaca aagaagcaag tcttagaaac gtgcatttct ttcataaaaa
    17821 gaaaaaattt gaaatctttc caaagaaaaa gaaaaaacta gtgatacttc ctctgaaggg
    17881 taatattgga atgggtaaaa aagaagagga agaaataaaa tcggatgcaa tccaagccta
    17941 ttctgtgatt ccagttcttc gttccattcg aaaggataaa tcgataatag gtgtgatttt
    18001 agaaattgat tcttctggtg gttctgcctt tgcttctgaa ttgatctacc aagaaatttt
    18061 aaaactcaag aaagaaaaaa aagtatatgc ctattttcaa aatgtagctg cgtcaggcgg
    18121 ttattatatt gcagctgctt gtgataaaat tatatcgaat ccgctttcga ttactggctc
    18181 gattggaaca atcatgattc gtcccaatct aaaaaaatta tacaatgaat atggagtttc
    18241 taaacaaaga attgaattct atccactcag agaaattttt tctgaatacg gacaaatttc
    18301 caatgaagga aaagaatttt taacagaaga agtaaaaaga gtcaatggtc aattctatga
    18361 aagagtcatg ctttctagaa ataagacttt aaaagattta gagaccttag cagaaggaag
    18421 agtttttact ggaaaaagtt ttctggcaag tagcatggtc gattccaatc agtctcttat
    18481 ggatactgtc gaagaactaa aaaatgagtt aaaggaaaaa agaattttag ttcagtatct
    18541 tccttctact tacagtttaa aatcaatggt gagagaattt agactgggag ttcgactttt
    18601 attaaaccca aaactcattc taaaaagaat cactcgttcg actgacgttc aattaaaaag
    18661 cgatctcgcg gatgtagttg cagaaaattt atttcgaaga aattgaaatc aatcggtagg
    18721 cgtggccttg cgcctttatt tccaccacga agagcacgaa gaaacacgaa gggctttagt
    18781 ttagaaggtt ggatttaagg agattttgga attcgttcaa agtgtcattc tcgaaatcct
    18841 ctataggaaa taactatttg aataaaatat tcttgaacta atcgatagtg gaatagtaaa
    18901 tcaatgtttt attcctaaaa atgtcgagca gttcacgcta cttgcggcta tttcgtttaa
    18961 atatttctac cacgaagagc acgaagaaac acgaagggct ttagtttaga aggttggatt
    19021 taaggagatt ttggaattcg ttcaaagtgt cattctcgaa atcctctata ggaaataact
    19081 atttgaataa aatattcttg aactaatcga tagtggaata gtaaatcaag aattcatttc
    19141 tcagtttgta gtttcacttc cttaaaatgt agttagcgaa atataaaatc aagatttcat
    19201 ttctcaattt gtagtttcag ttccttaaaa tgtagttagc gaaacaggaa atcaagattt
    19261 catattaact acgcatgaac tggtgacttg gtatagtggt tcgtatagtg ttgctatagt
    19321 ccttggtata gtgaaacgat gttttgagta tgggaggaag tattgtggat aatattctat
    19381 atgataaatt gatttattcc aattgcctga atattcctat tgacttttgg ctaggaataa
    19441 agggaattag agttagatag tgtagctagg tttaagttta gaagcccatt gactctgttt
    19501 agcaaagaaa atacagagca ttctaatgcg tttttagaat taatgtctga gggatgatag
    19561 aatagcaaag aagggaaaaa agcaagttgg atagtaaaaa aactaaaaat aaaaaagaaa
    19621 caaaagaaaa agcaatagaa gtaaatcttt gggatgcagc aaacaaactt cggggaagtg
    19681 tagagcctgc cgaatacaaa catgttgtgt tgggattgat ttttttaaag tttgctagcg
    19741 ataaatttga agagcagaga gctaaattaa tttctgaagg gaaagaaaag tatatagaga
    19801 aaccagaatt ttataatatg aacaatgtat ttttcttaga cgaaactagc aggtggagtt
    19861 ttttaatcag taaatccaag caaaataata tcgctcttct cattgataca gcactccata
    19921 caattgaaaa aaataataaa tcactcaagg gagcgcttcc tgataattat ttttctcgtc
    19981 tgggattaga cacgaccaaa ctttcgtcgt tattagatac aatcaatgag attgaaacaa
    20041 cgagtgatcc aaagcaagat gttgtaggaa aagtgtatga atattttctt tctaaatttg
    20101 cattggcaga ggggaaagga aaaggagaat tctatacgcc caagagtata gtaaatctaa
    20161 tcacagagat gattgaacca tacaaaggaa tcatctatga tcctgcctgt ggatcaggcg
    20221 gtatgtttgt tcaaagtata aagtttattg aagcgcataa cggtaataaa aaagaagttt
    20281 ctatttacgg acaggaatat acaaacacta cttataaact agcaaagatg aacctagcga
    20341 ttagaggtat cagtggaaac ctaggagaaa aagcagcgaa tacatttcta aacgaccaac
    20401 acaaagatct taaagcagat tttataatgg caaatccacc cttcaatcaa aaagattgga
    20461 gagcagaaaa tgaactaact gatgatccgc gttggcaagg ctatgaagtg ccgcctacag
    20521 gcaatgcaaa ctatgcttgg attttaaata tggtcagtaa actttctgaa aatggagtag
    20581 ctggttttat tcttgcaaac ggtgctttaa gcggtggggg aacagaatta gaaatcagaa
    20641 agaaattgat tacaaatcaa attgtagagg cgattttagt tttgccgcag gatatgtttt
    20701 atacaactaa catcagtgtt actctctggg ttttaaataa aaataaaaaa gctaaatcaa
    20761 aaaaagtggg tgatgaaacg cgcaattata gaaatagaga aaatgaaatt ctattcatgg
    20821 atttgaggga aatgggaatt ccttttgaaa agaaatacat tcaattttcg gaggaagaaa
    20881 ttcaaaagat tgcagacacc taccacaact ggcaacagga aaattacaaa aaaaagtata
    20941 aagacattcc tgaattttgt tacagtgcca ccatggaaga agtcgcaaac aaagactatt
    21001 cacttgtccc cagcaaatat atcgaatttg taaacagaga tgaaaacatt gatttcgatg
    21061 aaaaaatgca atctctccaa aaagaaatag gagagttact caaagaagaa tccaagtccc
    21121 gaaaagaact tttgaatgta ttcaaggaat tggggtatga aatcaaatta taaaagaatc
    21181 ggggatttta taagattagt agatgaaaga aatactggtc ttaaagttca agaactgatt
    21241 ggtcttacaa tttcaaaaca attcattccc tcggttgcta atacgattgg ttctgatatg
    21301 gaaaattata aaattataag acaaaatcaa tttgcttgta gtttaatgca ggttcgtaga
    21361 gataaaaaaa tccctgtagc acttttgaaa gataaagaag ttgctattat atcgcaagca
    21421 tacccaattt ttgaaattat taatacaaaa catatactac ctgaatattt gatgatgtgg
    21481 ttttctagaa aagagtttga tcgcgaagcg tgtttttatg ctgttggtgg agttcggggt
    21541 agtttggagt gggaagattt ttccaatatg aaactccccg ttccttctat cgaaaaacaa
    21601 aaagagatcg taaaagaata taatgtaatt ctaaacagaa tcgaactcaa caatcaattg
    21661 attcaaaaac tagaagaaac cgcacaggca atttataaac agtggtttgt ggatggagtt
    21721 gataaggata atttgcctga ggggtggcga gttggaaagt taagggaact atgtgaatac
    21781 tcaaacaaac gaattcaaat agaaaaactt aatttaaaca attatatttc aactgagaat
    21841 atgatacaaa atagaggagg aataacaaaa gctaataatc tacctaatac aaacaatttt
    21901 acaaattttg aaattggtaa tattttaatt tcaaatattc gaccatattt taagaaaatt
    21961 tggttagctg attttaatgg tgggtgttca aatgatgttc tctgccttgt tccttttaat
    22021 aatatacctt ctttgtatct atatcagata ctcgaaaaag ataatttttt tgattatgta
    22081 atggcaggaa gtaaaggaac caaaatgcca cgaggtgata aaaagtggat catgaattat
    22141 ccagcgatca tcccagataa aaagatatta gagaagttca acttaatatt aaccaatatt
    22201 caaaaaaaga tttttctcat gaaacaagaa gaaataaaat tattagaact agaagatttt
    22261 tttgtgtcca aactagcaac tgaaggcagg ggatgaaaat gtttatcaat cgaaagaaca
    22321 gatgggaaaa tgaaagaagg atttttattg tcccgggatt gaatggtaaa taataattat
    22381 atgaatttat ttgaaataga aaaaaaagaa atcgaatctg ttactatctt ttgtgatgaa
    22441 agaaagaata tcaatggtca atggaattat ttaattcttc tgattatacc aaccattcaa
    22501 ttagaaacag cactggcaat acttacggaa aagcggaatc gaattggtta caattttgag
    22561 ctgaaatttt cagatataaa aaaagatcat ggagataaat ttttattagc gagtgaatgg
    22621 attgatttaa taaagaagga ttctcagaag atttttcatt ttaaaatatt aggaatagat
    22681 attagtaaat tcaaaaataa aaattttgga ggagatgtaa agaaaggaga atattctgcg
    22741 aatatgtaca atcgtttttt cagaacaaat ttattaggat taaagtattt ttttaatcca
    22801 ttaaatattg ataatatttt tcatgataca gaaggcaatt tagaaaacca ttcctatttc
    22861 aattggcatt cgatatggaa ggtaaatact accgaggaca atataactat aaattctaaa
    22921 agaatttatt ttataaactc caatcataat ttagaagcag aatattcgaa ttattctcag
    22981 ttgatccaac tttcagatat agttttggga agtgtttcaa attgctttga taccaatatg
    23041 aataatattg gacaacaaaa actatccttg aaaataaaag atttagttga aagaataata
    23101 aacaatccac tcaataaaaa cagtagctat aactattata aaagatgtga tatatctttt
    23161 ttcccaagtg aacctattca tgagcctgaa tcttcgcaag ctaaattaga gggttggata
    23221 tatagaaaaa gaaatctagt gattaattcc tctgaaataa aaactaagat agacaatcaa
    23281 cttaaattta aaatggaata attgaatatg tgttcggata tattattgga atttaaaaat
    23341 tttatcaact taaaaaacat attcgaaaat tggtcaatag acgttatgcg atttaaaaag
    23401 aaggttccag taaatgtgcg gtatctccag tcaattggat caatgcaaaa tttagaggaa
    23461 atgatggcag ttatgattct aatcataaat tttgaactgt atatggggag tgttttgata
    23521 tcaatgaact acactttctg gaaaacaaaa ttcgaggaat cattagattt tagtaacaaa
    23581 atagatcatc agttttgttt ccattacata acaaaaatac atgagtattg ttaaaaaggc
    23641 tttaatttaa tatgaccaac tctccccaca acagtgaaat tctaatctat caaaatccag
    23701 aaggcaaaat caaaatcgat gttcgtctgg aagatgaaac tgtttggtta acacaagctc
    23761 aaatctgtga actctttcaa aaatcaaaat ctacaattag tgagcatata aaaaatattt
    23821 tcgaagaaga ggaactagat gagaaactgg ttgttcggaa tttccgaaca accagtcggc
    23881 atggagctat tccagaaaaa aagcaggaaa tagaagtaaa aggttacaac ttagatgtaa
    23941 ttatttctgt tgggtatcga gtaaaatcta cgcaaggaac taagtttcga atttgggcaa
    24001 cccaaagatt gaaagatcac attattaagg gatttaccct caatgaggat cgattcaaat
    24061 ctgggaattc gatgaattat ttttctgaat tacaagaacg aattcgggag attagacttt
    24121 cggaaagatt tttctatcag aagatcaagg atatttatac aacgagcatt gactatgatc
    24181 caaaagatga gaagactatt gaatttttta aagtagttca aaataaattg ctgtgggcaa
    24241 taagcagtca gacggctgca gaattaattt ataaaagggt agatgcaaat ttaccgttac
    24301 taggtatgca gtcctttgat aagaaatcta atgtatctat caaaaaatct gatgttggta
    24361 tagctaagaa ttttttgaat gaagacgaaa ttaaattact tggattattg gtggagcaat
    24421 atttggcatt tgcggaagct atggcacaac aaaaaattcc tatgtatatg aaagattgga
    24481 tttccagact cgattctatt ctacaattaa atggacgaga actattaaca catgcgggaa
    24541 gaatcagtca tgagatggca atccaaagat cgcaagaaga gtttgaaaaa tataaacaag
    24601 cagaaagaga aaaacaaaaa gaagatagct taaaagaatt agaagaggat attcagaaac
    24661 tgaataagaa atctattcaa aatgagagta aaggaaaata agttctgaag tagggctaaa
    24721 cacaatgaaa ttcactgaag aaaaattaga acaaaccttt attgagttgc ttaagaacga
    24781 agggtattca catattctag gaaataacgt tactcgaaaa atggatgaag ttattctaga
    24841 agatgattta cgaaaatatt tattcactca ctatttatct gaaggaatta ctctaaacga
    24901 agtagattca attttactca aactcaaatc tcttccttct tctgatcttt acgaaagcaa
    24961 taaacttttt atacagtata ttacagatgg ttttattcta aagagagaag atcaaaccca
    25021 aaaagatatt ttgatacatc taattgatta tacggaagca gataagaata tatataaatt
    25081 tgtgaatcaa ttagaaattg taggcactga aaaaagaatc ccggatggaa ttttatttct
    25141 gaatggttta cctttggttg tatttgaatt caaatctgca atccgagaag atgcaacgat
    25201 tcatgatgcc tatgttcaat tgacaactcg gtatcgaaga gatattcctg aattatttaa
    25261 atacaacgca ttttgtgtaa tcagcgatgg tgcaaataat aaagcaggct ctttctttgc
    25321 tccctatgag ttctattatg gatggagaag agtggctggt ttagctaaag acgtagatgg
    25381 aataaatagt atgttcactc tcattcaagg aatgtttgat agaagtagac ttcgtgatat
    25441 cattcacaat tttatcttta ttccagattc aagtaagaaa gatgaaaaga ttgtttgccg
    25501 ttatcctcaa tactatgcag ctagagcatt gtatgagaat attttaaaag cattaaagcc
    25561 cgagggcaat ggaaaagggg gaacttattt tggagccact ggctgtggta agagtttcac
    25621 aatgcttttt ctaactagat tattaatgaa aagtgaacat cttgaaagtc caaccattgt
    25681 tttaataaca gatcgtactg atctagatga tcaactttca actcaattta tcaacgctaa
    25741 aaaatttatt ggagacaata cagtagaaag tgttgaaagt cgtgcagatt taagagagaa
    25801 attacagggt agaaaaagtg ggggagtatt tttaactaca attcataaat ttacagagga
    25861 catagaatta cttacaacta gaacaaatgt aatttgtata tcagatgaag ctcacagaag
    25921 tcagataaat ctggatcaaa agataaaaat aacttctact ggagttacta aaacttttgg
    25981 atttgcaaaa tacttacatg attctttacc gaatgcaacg tatgttggtt ttaccggaac
    26041 tcctatagac gcaacgttag atgtctttgg aaaagtaata gatgcatata caatgacaga
    26101 atctgtaaga gatgaagtaa cagttcgaat cgtttatgaa ggtagagctg ctaaagtaat
    26161 tttgaaaaat agtgaattag aaaaaattga acgttattat gaagaagccg ctgaactagg
    26221 agcaaatgaa taccagatag ataaaagcaa agaagagaca gcaaacatgt atgcaatttt
    26281 aggagatcct aaacgattac aagaggttgc tcaggatttt gtaaaacatt atgaaaaaag
    26341 aacttcggaa ggctcaacag taaatggtaa agctatgttt gtaagcagta atagagaaat
    26401 tgcttatcaa ctctataaaa atattatctc aatcagacct gaatgggctg aaatacgaga
    26461 agcagatata ggagtggaac taacggaaaa agataagaaa gaattaaaac caattgaaaa
    26521 aattaaatta gttatgactc gaaatcaaga tgattctaaa gaattgtatg gtttattagg
    26581 aacaaaagat tttagaaaag aattagatcg acaatttaaa aatcccaaat caaattttaa
    26641 aatcgcaata gtagttgata tgtggctcac tggctttgac gttccttttt tagatagtat
    26701 ctacattgat aaacccatac aacagcataa tttgattcag actatttcaa gagtgaatcg
    26761 tagatatgca ggtaaaaata aaggactagt tatagattat ataggaatca aaaaacaaat
    26821 gaatttggct cttgctaaat ataataaagg agatgaggat aattttgaag atatagaaca
    26881 gtctattgta gttgtcaaag atcacttaga tttattaggc aaattaatgc atacgtttga
    26941 taattcaaag tattttaaag ggagtaatct tgaacaactc actacattaa acttagcggc
    27001 tgagttcgtt cagcaaacaa aagacgttga aaatagattt atggggttag taaaacgttt
    27061 gaaagctgca tatgatatat gtgttggaag tgaaaagtta agtcaaacac aaagggatta
    27121 tacacatttc tacttagcaa ttcgctccat tattattaaa ttaactaaag gcaatgcgcc
    27181 tgatgttgcc caaatgaatg ctagagttcg agaaatgatc aaagatgcat tacaaagtga
    27241 tggtgttgag gaaattttca aattaggaga agaagaatca gaacaagata tattcaatga
    27301 agattattta gcaaaaatta ataaaattaa attaccaaat acaaaaatca aattacttca
    27361 aatgctatta gcaagagtca taggtgaaat aaaaaaaatc aataaagtaa aaggtattga
    27421 tttcacaaaa aaaatgcagg ctattgtgga acgttataat aatagaaatc agaatgatat
    27481 ttttaaaagt gaagtatatg aagaaatggc tgaagcttta acaaatctaa tctgggatgt
    27541 tcataaagag tttaaagcag gtcatgaact tggaattgat tttgaagaaa aggctttcta
    27601 cgatattctc aaagaattat gtataaagta tgactttaaa tatcctgaag acaaaatgat
    27661 agctttgtct aaagcagtaa aagtgattgt agacaatcaa tcaaaatttc cagattggaa
    27721 caaaagagat gatattaaat ctgcattgaa agttgaattg attttgatac tagatgagtt
    27781 tggttatcca cctgtagaaa gagatgaagt atatatagaa atttttgaac aagcagagaa
    27841 ctttaaaaag aataaaaaat aaagtggata gacctgctta ctacttatta aagatatttg
    27901 aagtataagg ggacttttgc aaatgggata agagcatatc tcaatccgaa attattcaag
    27961 aatttcaaat cacttggaag tcttttggat ctaatgttta agaactttcc aaatagacca
    28021 aactctttct tctcttcgcg attggttgtt acctatgctc atgaagagat aggtgagtat
    28081 agaaaaaacg aattgaaagg tttttagaaa taaatttcga attataaaga aaggaaatga
    28141 aaaatccatt aatttaacat taagttttag attgagggca gttttcttag tttagcacgg
    28201 agcgattaaa agaagacgag attaattcca ttcaatcatt cataaaaccg aaaccccgta
    28261 gggataatag gtgtttgtct ctacggggga tttcatttta actaaaatat tttttcagtt
    28321 atttctttgt ggaacaggta ttgattccaa gaggaatgta gagaaggcaa attcctgtgg
    28381 cagcagttcc agcaagaaca gctcctgcaa tccaaagcac aatagcgatt gttccagttg
    28441 caacaaatcc accaataatt agaccagctc ctagtatagc tcttatgatt ctatctatgt
    28501 ttccttcatt tactttaaac attgtattct ccttctatca gtaaacagat tcgaatttca
    28561 aatttgtttt tttaaattgt ctgatatatc tcaaatataa acgatgattt taaaatctgc
    28621 aagagttgaa tcggacaaat atgaaggggt atctggaaag attcaaattt gtgaaatgac
    28681 attgcaatga tttttatttt aattctactc aataaaatac aggtatttat atgtttaaaa
    28741 ttacattttt aggtttggaa gattccatta gttctttcat aacaggtcca ctggatttat
    28801 tttatcatac aggaagaatt tggaatgtta tcaatggaga caaggaagac ccattttttg
    28861 aagtagagtt ggtttctttg gatggaaaaa aaattacctg tcaaaatgga attacccttc
    28921 agcctaaatg ctctattttt gattcagtgg agcaagactt aattgtgatt tctagtagtt
    28981 cctttgatat tgggtggact ctagagaaat ataaacctgt tctaccatat ttaagatccc
    29041 aatttgaaaa aggaattcca gttgctagtt tatgtattgg agctttttta attgcggaag
    29101 ctggattgtt gaatggaaga aaagcatcca ctcattgggg attttgcaat ttatttgaag
    29161 agatgtatcc acaagtaaaa ctttgtagag aagaactgat ttcggaagat actggagttt
    29221 ttacttcagg tggagcaaat tctggattag atcttacact ttatctaatc gaaaaatttt
    29281 ctggtcatag tcaggcaatg aatgttgcta agtcttttgt tttagaatta ggacgacatg
    29341 gacaagcacc tttctccaag tttattggtc gtagaagtca tagcgacaaa gagatgttaa
    29401 ttgcacaaga atggattgaa aataattgta atcggttgct ttcctttaat gagatttctg
    29461 aaaaacttgt gatgagtcag agaactttaa atagacgctt caaagaagcg acaggtctct
    29521 ctcctgtaga ctatttacaa aatgttagaa ttgagtcggc aaaaatcttt ctcgaaaccg
    29581 gaaatgatac agtccaagaa attgcttatc tagtcggtta tgaagatatt agttttttta
    29641 gaaaggtttt caaaaaaatc actggaatca caccgaatca atatagacaa atgtatcgag
    29701 ttcgaaatca aatacagaat gggtttcatc cctaggataa ggaatgacta agaaatgatt
    29761 cagaaaggac ttaaaatgaa aagggtaatt gctatactct ttttatttgc tatttttaat
    29821 tgttctatcc ctaaaaatca tattcaaagt tttgctttgg aaaacaggaa agatagatta
    29881 gaaactttgc agaaatattt aattttcaag tcagaagttc tagatgcaga atatgatatt
    29941 tatgatgtga atctaaatgc agagtataca attcctggag caacatctag agactatcgc
    30001 attgtattat ttctaaaaaa agaggatatt tccaaatggc tcacagatgt aacactcaca
    30061 tcctttcctg agagtgatct ttggagagaa gacttgattc aaaaaagcaa tagacttaag
    30121 tcattgcaat tagagaaaga ttatatgact tataaaaatt caaataaaac tattttttac
    30181 aatcaaaaac attccatttt atttatacgt atcaatcaaa attaaaattc tctaagccgt
    30241 gcttgtgctt ttaagataga agcatacaat gttgattcga gatctaaaat cgaacactaa
    30301 agatttgatt gactaaagtc agaagcaaac cagtaaatat aattttatga aaactgtaat
    30361 cctattgttg acttttcttt tgttaactac cagttcttgt tgggatgtag gttacggacc
    30421 ttacggaagt gaaaaaggtt actgtgaata caataagtat aatgatagtc cttctagctt
    30481 ttgcttggca atggtcttag gatctaatag tgagagttct aagaaatcac aggcattgat
    30541 agatgcctgt ttggctgttt tgattttaga agaaaattca tgtgatacca aatccactat
    30601 taaacggtat tggttttaaa aatccattca aaaactatta atttcttact agctaaggca
    30661 gcgagaaatg cgttagctac ttaattcttc ccagcggagg agtttggatt cgagtaaaga
    30721 ttcaatttcc gcgagtctat cactgagtga ttttacttta acaaaatcag agtggaacgt
    30781 tgtaagatca gattgaatct gagttttttc tttttcaaga gagtcgatat ctgattcgat
    30841 tgtttttaat tctttctctt ctttgtaaga taattttttc ttttgtggtt tatttgtatt
    30901 atcctctact tgcttttttt cttgaattgg ttctgatttt tcttttaggt cttttttggg
    30961 tttactcaaa tattctgaat aggttcccgc aaatccagaa atattaccca ttccatcaaa
    31021 aactaaaaga tgattggcaa ctctatccat gaaataacga tcatgggaaa cagttagaat
    31081 acaacccgga aattctaaaa ggaattcttc tagaatggaa agagttttta tatcaaagtc
    31141 gtttgtaggt tcatctaaga ttaaaaaatt tggattgtgc attagaattt gcacaagata
    31201 aagtctacgc ttttccccac cggataattt ttctacagga ctggattgca ttttagatgg
    31261 aaattgaaat ctttctagta gcaaaccagc actgattctt tctccatttt ccattggaat
    31321 atattcacct gctgtttttt gaatgtattc tagaactcgc atcgattgtt ttagttcaat
    31381 actgttttga tcaaagtatc caaaccgagt attcattcca ggatttacaa aaccactatc
    31441 aggatttaat tttcctgtta gtatattgag taatgttgat ttgccgcttc cattgggacc
    31501 tacgattcca agtctctcat agtgtttaaa tgtataactg aatttctgaa ttaaatttct
    31561 tggtccgaaa gatttagaga tattttcaat ttctagaatt ttctttccta cctttttggg
    31621 gtcgacttta aatgtaaatt tctcttgctc ctcaaacttt tctcgattga ttacgcttat
    31681 gattttatcc gttcttgctt tttgttttgt ccctcttgct ttcggctgac gttttaacca
    31741 ttctaattct actcttaaaa agccttctgc ttttgcttct ttcctagcat ttgcctcatc
    31801 catctctact tttttttcta agtagtaatt ataatttcct tcgaataaag tagactgagg
    31861 aagtgaaatt tctagaattt tatctaccac taaatctaga aaatatctat catgtgtaac
    31921 aaggagtagt gatttattca ttttgattag atactcttct agccaaagaa tcgtatcaat
    31981 atctagatgg ttggttggct catctagaat gagtagattg gattcatcga ttaaagtctg
    32041 gacgagttct acttttttta gcattcctcc cgaaagttct gacatcttcc gatttaagcc
    32101 tttaattcct aattcattta gaatcgattt aatttgattt tcatattccc aagcatttgc
    32161 tttttcgatt tcattcataa tcgaatgcaa ttcgctttcc aattctttgg tagactctaa
    32221 tgtaattctt tctgatatct cttcgtattt ttgaatgagt gataatgctt tgcttttgcc
    32281 tttaaaaatg tgttctagaa ttgtatcctc tgggttaaag ctaggatttt gctcaagcga
    32341 actaattttt aaatttctgt ttttatagac tgcaccacca tcgacttcct ctagaccttt
    32401 tataattttt aaaaaggttg atttgcctga gccatttact ccgatgattg caactttttc
    32461 tccttcattt agaccaaagg ttaaatctcg gaacaattct ttttcgccag cacttttaga
    32521 tattttatca atggatagta gattcatttt ctgtcatagt gctattgcaa gtataggaga
    32581 caatcttaat tcatttttca tttgcctgaa aatattcacc tgtcaatttg gtgaatggta
    32641 tgaaaaaaaa atctaagaat gaaactttga aaactatttc taaaaaagcg catccagcta
    32701 agaaaaaacc gacctatgaa gtaagtcttg caattgcagg cggcggatgc aaagcgttat
    32761 acggaatggc tgttggatac aaacttagaa aatggggagt taaaataaaa gaaatttctg
    32821 gagtaagtgc aggcgctgct atggctcttg gaataatttc agaaacagaa gatgaaagta
    32881 tcgagtattt cagtgaactt accaagcgga ataaatcgaa ttttcaagtt atgagtttgt
    32941 ttcaaggaaa aagtcctttc ccgcatgaaa atatatatag aagactcatt cgacatggca
    33001 tgaatctgga gaaagttcga aaatctaaag tgaaaatata tattggaacc gtgcaagcaa
    33061 ttcccaaaaa atcaggtctc aatcattatt gggatatagc aaaattagta acagaaacta
    33121 ccagagctta tctactcgat gaaaatgata aagaggaagg cattccatgc aatcgagtca
    33181 gtactattat ggaaagatgg aatatgattc caatcgttta tacaaataaa gatttaaaat
    33241 ctcctgaagt tgtggaaaac attatattga actcatcttc tattccgcct gtcgtatctt
    33301 ttcaaaacaa taacaaagtt tattttctag atggaggact tacaaataac ttactcctag
    33361 aaagattttc taaaaaatca aaaaagattg gagtttatta tgaagataca acaatctttg
    33421 gaaaagacag ggctttacta gagagcactt ttttaataaa gccttctcgt aaattaccaa
    33481 ttgcttcctt tgactatact gatccggtag cagtaaaaga atgctatgaa ttaggaaaag
    33541 aagacgcaga aaaattaaaa cctgaaattc taaatttttg taggaaggac taatatggca
    33601 aagctaagtg ttaatgttaa taaaatcgca acgcttcgaa attctcgtgg gggaaatatt
    33661 ccagatttac taaaatatac aagagttata atggatgcgg gtgctcatgg aattacagtg
    33721 catcccagat ctgatgaaag acacattacc aaaaaagata catatgattt aagaaaattc
    33781 attctagagt ataataaaga atacaataaa aaaatagaat ataatattga aggtgctcca
    33841 gatgaaaggt atattgacat tatcctcgaa acgaagcctg agcaagcaac gttagttcct
    33901 gttagagaag gggaaattac atccgatcat ggatttgact tagctagaga aggggagatg
    33961 ctctatccga taattaaaaa aattaaatct gctggaatcc gagtctctct atttttagat
    34021 acaaacctag acaatctaag ttatgccaaa caaattgaag cagatcggat tgaactttat
    34081 acaggtcctt ttgcccatgc atttgaatct gggaagggcg tagaaagttt tatggaatat
    34141 gaaaaaatgg caatagctgc tttaaaactt gggttaggaa tcaatgcagg ccatgattta
    34201 gatgagaaaa atttagctat atttaaaaaa ctaaacggtt tacaagaagt atccattgga
    34261 catagactca tgtcttatgc tcttttatat ggtttagaga atgcagtaaa aacatatcta
    34321 caagcactcg ttagataacg cgagttcaat gtaactaaga attaaaagac taccactgat
    34381 aaacacagat aagagatacg ataattcaaa attatccatc gtttattgat ctttcactta
    34441 acggttgcat cggtggtgaa aaaaactcac ttactcgaca ttttatctac accattccaa
    34501 tcagaaggaa gtccatattt agtgaaatgt tttatttttc ttaaatagac ttcagatggt
    34561 agatcagagt tattttcaag atagatggat ttaaatattc tgtgagcttc tctcagattt
    34621 ccctctatgt aataacgcat tccttgttca aaatcaggag ttatccttat tttaaacaaa
    34681 aaggtttttt catcatcacc atcaaagact tcataaatgg gaactgattc ttcttttccc
    34741 actacctgca ctagtcctat aaaacgcata tgaaatcttt ctggattgat taatttagat
    34801 tttgtggttt ctgtgattag agttgtgaca ccatagtatt tagttaaacc ctcaatacga
    34861 gaggcaagat tcactgaatc tgcaattact gttgtatcca ttctttgcac ttctccaatg
    34921 gtgccaagca ttaagtttcc agtatggatt cctactccta tatgaatcgg agcataaccg
    34981 ttgttagccc tttgtgcatt gtatgttttt aattcttgtc gaattgcaat tcctgcgagg
    35041 agcgcataat caggagtagt cggaaaaatt gccatgatag aatcgccaat gtatttatct
    35101 ataaaccctt tgttctctct gactaatggt cccattctct ttaagtaaga attgataaaa
    35161 ttaaaagttt cttttggggt catagattca cttaatttgg taaatgatct gatatcggag
    35221 aagaaaatag tcatctcttt atatacttgg tctccaagag agatttcaat tatactttgt
    35281 ttatctaaga aattgagaaa ttcaaaaggg acaaatctct tcatagaatc gagtagttgt
    35341 tttaattttt gatcataatc aacgatcgtt tctgccatat tattaaagtt atcagaaaga
    35401 agaccaactt catcttttgt ttcaatattg gcacgagcag aaaaatcttt ttcaccaaat
    35461 ttcaaaacga ctttgtttaa ttctaggatt ggtttcacaa ataaattacc aagtagaaga
    35521 gacgagatgg atgtgagtat tacagtaatg attccaatga tagcagatac taaagtggat
    35581 tttttgagtg cagttctaat atttgtatct gtcatatcta cacccagaac tgcaatttgc
    35641 tttcctgtct ttttctcttt tattggagaa tagcctgaga tggagtaggt atgaaactga
    35701 gcatcatacc tataatcttt ttcaacgaaa gatatatttt gtttaaatgc attttttgct
    35761 tctggaaatg cagaaatact gtatctacta ccataagtgg atatatcttc taacgtttcg
    35821 ccctttgctt ttttttcgag taatcgtaaa acatctgcat ccgctaaata aatcacctct
    35881 tcaggattat cagtgcttac ccagatatag acatattgaa tcaaatctgg ttttgaattt
    35941 ctgagtgaat ttaaagcatt gtagacatct aaaaattctt ttgatttttg aatctcgttt
    36001 actttttctt ctgataagtt ttcagataaa tttgaaatta gaaattgaag agagtcttgg
    36061 tcaatgagtt gagatccaag atcggaagta tggatcaatc tatctctgta ttcttcatag
    36121 agttcgtttt gtagaatttt aaaagatgtg tatgcgagta atacactaac aactaaactg
    36181 aggattagag aaccaattaa tatttttatt ctaagattca tttgtggaag ttcaattcta
    36241 gaaaacattt actaactcac ctttaacaag gtgaaccaag cgttcaagta tggtttattc
    36301 ctttcaccaa tgaaaaatga caatggaaat gagaagagta aaaaaaaagg aacagaggat
    36361 ttttcacaca tttctttttc actcatcgtc agccgagcga agttataagc gattgatttt
    36421 cttatttcat tagcatttaa tatcatcatt tatctctaaa gttttttaat ttgcacaatg
    36481 cctgttaata gttctcagaa tcaaagaata gcgtcaagaa ttaactaaat ttcatctgct
    36541 aggcttctgt attttgcaaa gaattcgtct tgctccagta agagcttggc agccctttca
    36601 atggcaagat atagatttgc ttcctgtttt gcttgggatt tttcgtcttt tagtgtttta
    36661 agaaattcat gaaagttttt tcttacaagt ttatcatttt tggattgaac tgcttttcga
    36721 aactcgcgcc aactcatagt taattggtgt ttcaaatctt cttctttttc gattggttga
    36781 ttttttgtaa aataggatgt atcccagatt gaataaaata aattcgattt attcttttct
    36841 gagtaataga agcttgcgaa attgacttta ttcttttttg aaacactcgt tagttcattt
    36901 attgattttt caggtaagca ttcagaaata aacccatttc gaattgattc agcggttaat
    36961 acttctttgc ctactaaatc cttagcaatt tggatggcta cttctttgtt ttgatttctg
    37021 cattccagtt ctaatttagt aattaagtaa ctgggatcat ttgttggaag agaatctttg
    37081 tttgcaattt ccattttccc attttccaag agagattgtc gatacatctc tacatacaaa
    37141 gcatctttgg ggcttttaat cactgctctt tctgaatacc gaacagctcc tgagaaattt
    37201 ttgattgcaa agaggtgaga tgctagattg taacatgcat gtggatcacc atcttcttcg
    37261 catcgctttt ctaacgtttt gactgcccag tctttttcat caggactgcc gtataaattc
    37321 attagaattt tttcatttaa tactaaatac tctgcttcaa gcccgggtgt aacagatttt
    37381 ttattttctg tggaactaca accggagcag gctgtcagga gtaaacattg taaaatcaaa
    37441 aaaaaactag aaatatactt ttccattttt tatatctcgt ctattcaaaa acagatacta
    37501 atctagactc atatttttct attaaatgtc ctaaatcgcc ttcatagtat aattctccca
    37561 attcgatcga aggagttgcc tttgttttcc caatgttata aatctccaat ttatttttct
    37621 gacaaatgga ttctatttga tcgaaatttt gggttgggaa agtgataaca acagaggctg
    37681 ctgtttctcc gaaaagagtt tcatctagtc gtgttttttt aatggaagac aaatcaatgt
    37741 tagccccgac tttctttgga aaagccattt tagagagggc aaccaatata ccaccaaggg
    37801 aaatatcttt tgcactcgaa atgagtctat cttgatttaa gcttaaaatc aaattttgta
    37861 aattcatctc atcttctagg gataattcgg gaatgtttcc ctttgtaaga ttgtgaaatt
    37921 cttttaagaa ttcacttcca cctaaagtag gtttaaatct accgactatc gcaatggttt
    37981 catttggttt ttgaaagtaa tttttgatat ggtgagtaat atctggaatt aaaccaacca
    38041 ttccgattgt aggagtagga aatactggac cttcaggaga ctcattgtaa aatgaaacat
    38101 ttcctcctgt aacaggaagc tttagaaaac gacaggcgtc accgattcct ttcacacatt
    38161 cagagaatac atagtaattt tctgggatgt atggatttgc aaaattgaga ttgtttgtga
    38221 taccgattgg ttttgctcct gttacaaaaa cgtttcgagc cgattcgcag actgcccaga
    38281 tagctccttt gtatggatct aaataggtat agcgagaatt gcaatcagta gagacagcga
    38341 ttcctttatt tgttccagga actcttgcaa gacctccatc aagtccagga ccaattactt
    38401 ctacaagtcc tacatcggta tcatattgtt catagagtgg ctttctagaa catatgttta
    38461 aattggaaag catttgatct agagtcttac cgatattagc cgctgtgata tcctgaatac
    38521 cagatatgtc taacttggtt acttcatcta aataattcgg tcttttttct tctctgacat
    38581 agcggggagc gcctccacct aagacaagag tatcggaagg aatttctgct tttactttgc
    38641 catctttctt gactctaagc atcccatcct ctgttacgac tccaatttcg actgcattta
    38701 aatcccattt cttaaagatg gcgactaact cttcttcttt tccttttttt ggaacgacta
    38761 gcattctttc ctggctttcg gaaagcattg cttcataggc attcattcct gtttctctga
    38821 atggaacctt atctagatct atctccattc cagtttttcc tttcgctgac atttcagaag
    38881 tagagcagga aataccagct gcacccatat cttggattcc aactaggata tctttctcaa
    38941 tggcttcgag tgttgcttcc atcaaaagtt tttccataaa tggatcgccc acttgaacgg
    39001 ctgatttttt ttcttcgctt tctttactta aatctttaga ggcaaaagaa gctccgtgta
    39061 ttccatctct acctgttgta gcacctacga taaatacaga atgtcctact ttaccgcctg
    39121 tcgtagcttt tgccattcca ttttttttca caacaccaac tgtcattgca tttacgagtg
    39181 gattttttga gaaagaagaa tcaaaaaata attcgccacc gctgactgca atacctagag
    39241 agtttccata gtctgcaatt cccttaacag ctcttttgag taaatattca tttcgtttaa
    39301 cgtctggtaa accgaagcga agagagttta atgagataaa aggtcttgca cccattgtaa
    39361 aaatatctct catgataccg ccaactccag ttgcagctcc ctggtaaggt tcgactgctg
    39421 ttgggtggtt atggctttca attttaaata caacacctag accatcaccg atgtctaacg
    39481 cacctgcgtt ttcttcgcct gttgtagtca acataaggct agattctgta ggaagtgttt
    39541 ttaattttaa tatagagttt ttataagagc agtgttctga ccacatgcca gaaaaaattc
    39601 caagttctgt agaatttgga attctaccta gaattttaca aatatcttca aattcatttt
    39661 cttttaaacc atgctcaatc gcgtcttcta atgttacttt ttctttttcc atctgtgtta
    39721 aaaatctccc tcagtttcta gatcttgaat tttcaattct tctaatcgct tttgtcttct
    39781 tttttctttt ttggtttttg tttttctaat tttttctctt tgtataaatg ctttgctttc
    39841 ttttggattt tctttctctt ctaatttatc gcataaaatg gcgcgggctt tgtatcgatt
    39901 taaaccctgt gttctatgaa tcgaacattt gatttcgatt ccacttactt tatgttttaa
    39961 atgaacggca gtcgatacct tattgacatt ttgcccacct tttcctccag acttagtaaa
    40021 tgtttcttct agatcagatt cttttatatt caacttctgc attctagaaa gaagttgagt
    40081 ttgtttttcg ggtgatactg gaaaggaaat ggacatttta atttccgaac atttcaaagt
    40141 aggggaatgg ttttccgcta gcatctaggc aaacgaatgt tacgcgacag ttgatcactt
    40201 cgtttctgat tctagatttt tcattgattg aaagtgcaat ggttctaacg gttaatgagg
    40261 atttacctgt cttttcaatg gctgcatgaa tttgaatgat gtctccattc tttcctggat
    40321 atttaaaatt tacatcatcc atatttactg taacaatatt tgtataggca atcttttcca
    40381 tggcgtatag agctgctgct tcatcaatcc aggcaagcat gattccacca aaaagatttc
    40441 catgtgaatt taaatctttc gacatgacaa tgtgttgcgt tactaaatcc atgcctttta
    40501 tatttggttc ttccatactt attataccaa tgaagaaggg cagatgtcgt tcagaggaca
    40561 gccttcacat tcagttttat gtgccttgca aaactttcta cctaaaaaaa ttaaatagag
    40621 ggaaaaatcc atccagtatt taggatccac aacttgcatt aagtcttttt caatttgcac
    40681 aggatcttca gattttgtga gttttaaaac ttttgagatt cgttttacat gtgtatcaac
    40741 aacaattcct tctgaaattt tatgaatctc atttagaatc acatttgcag tctttcttcc
    40801 aacgcctgga aacagaatca gttctttgat tgttttagga agcttgccgt tgtattcaaa
    40861 taataattta tttgcaaagc cgcttatgga tttggcttta tttttataaa atcctgttga
    40921 gaaaataagt tcctcaattt ctttcagatt tgcttttgca aaggattcaa gtgaattgaa
    40981 tttttgaaag agagcagggg taaccgtatt tacgcgctca tccgtgcact gcgcactgag
    41041 aattacagca atgcataatt catagtcctt agtataatgg agaggacaac ttacaggacc
    41101 aaaatatttt ttgagacgct tgtttgtttc tgttgtgaaa tttaatttat ctggtttgtt
    41161 ttttaaattt gaaaccaaag tagatcagga gagaaattta aatttctctc tcctttttaa
    41221 attagcgatt aagcagattt agctgctgtt tttgcttttg ctccaacata ttttttagga
    41281 gcgatagctg ctaacgaacc accattgtct ttgatagcag atttgagtcc ttttttcttg
    41341 agagttctaa gagctcttgt agaaattttt acagaaaccc atctgttttc atcttctaag
    41401 aagatatttt tcttaacgat atttactttc cagattcttc tggttttttt atgagagtga
    41461 gatacagcgt ttccgtaagc tgttcctctg cctgtaacaa cacattttct tgccatataa
    41521 gatacctttt tgtttattct ttttggatgg gctctattgt caatgaaaag agagtaaaaa
    41581 ggacttttta gaaaaaaaga attggggagg atttctatcc attccccaat ttgagataaa
    41641 caagaggatt tattaaaaat tgaaaaaaag ttttaataat tgattataat aaagcaataa
    41701 ctgtgccaca tacaaaatag ttctgatttt atttaatcaa ctagtttaaa ttggcagaaa
    41761 gggcaaaact gcatagatag gatccgaaat gaattgtttt gaatcttcat ctcaagaagg
    41821 agtcaaagta ttcttataaa tgtgcgtata aaataagcaa tctgaaaaga atttagtaat
    41881 tctgacaaaa gaatttaatc ccaaaaaggg gtgaatcatt tccctaaatc taaacttatt
    41941 tagagactag aagggatttc ggaatttcct ttggttatga atcctctggc tattagcaaa
    42001 aaatggaaga aaatcctagt aaaaaaaatt caagagaatc taggaagatt ctccctattt
    42061 aattcaattt acacaatcgg tagaattttt aattcaatta agaattcacc aaaggctctc
    42121 tcaccaggag caattttaca gagtccagat tttggagtaa aaaatgcatt tcctgtggaa
    42181 gtcattggct caattgcaat ggattttcta tcttccggaa tataaacttg gaagtagggc
    42241 attggcattt gtgaggtttc tggaactctc acttgaatga gaagtctatc ctttggatca
    42301 taaattccaa aatataattc tctaaaattt cccgttaaaa gatgatccaa tttcaaactt
    42361 ccgattggct ctcctttcgt aaataaataa gaaagatctc ttttttcgag agacttgatt
    42421 ggcagagaat ttccatctaa aggaatataa tgcgttaggt ttgtatgaat gatacattca
    42481 tctaaatttg catttaacct taaataggga tgatagccat aggcaaattc ttgtatctga
    42541 gaagatggat tgtgaatttc tgtttgaatg gatagataat tgtcttctag agtataggtt
    42601 tcgctgagtt gagggaaatt tggatttacc tcagtgagtt caatatgggc tgaatttttt
    42661 cttacttcga taagtcttct ttttttattg aaataaagac catgaatcgg ctgactatga
    42721 gtgtcttttt ctaacggctc aatgctaata gtctttccta caatttttag tttgtctgat
    42781 tcaagacgat tcacccaagg aaacattaaa aagtttcccg ataaaaagaa tgatttgttt
    42841 ggatcaaaag gtgagactac cggaattttt tttccagttt tgggatgtct caaactaagc
    42901 ttattgattt gcgcaccttg gtctaagttt aattctaatt ctgaacttgc atttaatagt
    42961 gtaacttttt gcatgattta attaagtaga atatggtttt gcgattccga atccttgcgc
    43021 atagtctatg cctaattttt ctagaatctc catcgtttct aagtcctcca caaattctgc
    43081 gattgtctta agtccaacga tatgtgcaat cttattaatg gactccacaa ttgcataatc
    43141 aagtggatca tctttcatat ctcttacaaa acttccatca atttttacat aatcgactgt
    43201 aaaatttttc aaataggaga aagaggacat accagatcca aaatcgtcta gagcaaagta
    43261 agaacctcgc ttcctaactt cttttataaa atcgagtgct tttttaaaat cggaaatagc
    43321 aactgtttct gtaatttcaa agcaaattag atcaggctca attccgtatt ctttcatttt
    43381 acgaaaagta aaagaaaaga agttatgatc attcatactt gcaccagata agttaataaa
    43441 aaatcgagtt tctcgatttc gagaactaga atctccgaaa tttttagata tatatttgaa
    43501 tgcattttct acaacccatc gatcgagtga tggcatcata ttataacgtt ctgctgctct
    43561 cataaaaaca tcaggcatga ttacagtgtt gttatttgtt ttgagtctga gtagaaattc
    43621 atagaataat actgagcttt ctttttgata gggaacagac acaattggtt gctgaaacaa
    43681 tgtaaattga tctagttcaa tgctctcatt gagcctagag aaccattgca tttcctgcat
    43741 tctaaatgtc atttgcctat catcttctat ataaatatga gccctgtttc taccggcttc
    43801 ctttgcggca taacaagcca tatctgccat actcagaatt tcattgattg tttctactct
    43861 ggggctaatc ttgattatcc caatacttcc cccaataacg aagattttat ccatccaatt
    43921 gaatctaaat tcggagagat tttttagaag attttctgca aagctatttg cttgctctaa
    43981 actatgattt tctaatataa ttacgaattc atctccccca atacgagctg cgaatccatc
    44041 tttttttaga ctttgggtga ggataacaga gacttgcctg agtagatcat ctcctgccgg
    44101 atggccacag gtatcattta ctactttgaa ttgatctagg tcgatataga gtaggctgta
    44161 ttgtagatct gaatctgatt ttactgcttc tagaatttta atcagtctac tttcaaattc
    44221 atagcggttc gataaaccag ttaaaggatc atgaaacgcc atatagtaaa cagcttcttc
    44281 tgccattttt cttgcatttc tagaatcatt ttctctcaat tccctttcga tcactggaat
    44341 gaggcgagcc atattatctt tcatgatata atctttggct cctgctttca ttgcctcaat
    44401 tgcgatttct tcgttcattg caccagaaac aattaagata ggaatatcga tattattgtc
    44461 ttgtgtgagt ttaattaagt ctagtgtgtt gaaatgagag agtgtgtgtt ctgtgatgat
    44521 caaatcaaag gtctttttgg ttgcagtctc tttgatttgt gttaaatcag atagtttcgt
    44581 aatctcaggt ttgtagtcac tatttattaa atattgtttg agaagttctt cctctttatc
    44641 tgattcatcg atgagtaaga ttcgcaggtc tttcatagtg atttatatct gtggtttaaa
    44701 attgacaatt gaatttaatt tttaactctc ttagcgatta caaaaattta cttaaagaag
    44761 ttttttattg aatcatttaa attgtcaaaa gtagatttta gtttttcctc tacttctgct
    44821 gttttctcgt tccattcatt tgatgtctta gtgaattgac gatctagttt tatgatacct
    44881 tcttcgatat catttagaag ttttttacgt tttgattttt ctgcttcatc aaaattccca
    44941 aattcatact ctattatatc gtcttttaca ctgcccttag ttagaagatg catttctact
    45001 tttacatatt tctgtaagag tgcaattctt ccttctttat ctagtcttct aacgctcaca
    45061 ctgagagaag ttgattttag aaatttgagt ggtctaaatt tacttgctct ttccactaga
    45121 tcatgatctt ccgcaattac tacactctca tcaaatccac caattctatt aaatagtctg
    45181 cgattgataa aaatacaaaa gccggcagca cgtgggttta aattttgatt tatttttaca
    45241 aataaattgg aaagttgaaa tactacttta tctagagtaa gttcggataa tggtaggaat
    45301 tcgcaggtcg ccaaatctat gtattgatcg tccatttctg taattgcttt ttctagaaag
    45361 tctgtcggta gtaaaacatc tgagtcgaag aaaaataaga attcacccgt tgcaactgct
    45421 gctcctttat ttctacccgg acctggcatc ccaccaggaa ctacctttgc gccatagctt
    45481 ttagcaatct ctatcgtatt gtcttttgag cctgcatctg ctactataat ttcgaaatcc
    45541 ttaaaagtct gattcttgag agattccagt aggataggta aatatttttc ttcatttaaa
    45601 gctggtataa caacactaat tcttggcata tccacaaaat attttataaa ttaaaaaggg
    45661 caagaacctt ttttaaaaaa gagtagaaaa tcattgggcg aatccttgca taaggtttga
    45721 ttttatttct ctaggttttt caaatattta tttgatttct tgccgttcta gtaaagtttc
    45781 ggataacagc tatttaccta acgattcctt tctgagtgag ttcgaatcca attcaaaaga
    45841 attctactcg ccataaaatg gtgtataaaa tggatttagg atatattcca tttttttatt
    45901 ctaagcaggt ataaaaacgg gaagtttacc cctttaaatt cttactattt ttacttttct
    45961 tccagtttgg gaatgcgttc tgtaagaata attaggtttt ctttttagct tttcgtatat
    46021 cggtaacagg agtaaaaaga gttttataga tttcaattct catatcattt gtttgagact
    46081 ttggttttaa ttctctaggg atagaaactt gcagagaaat gttagagaat atagaggata
    46141 acaatcatta tccgcatttg tgtttcaaat tttctatcag ttagaatggt aagaattgga
    46201 tctttttttt catatacttc tttgttcttt tgagtccaat gattgctttt ggaaattggt
    46261 aaaatacaag atgaatttta aattagaatg acaaatattt tacaaaaaaa tgaatttcca
    46321 aagataaatt ctttttcgga cgagaaagaa taatttaaaa aagaggaatt agaagataag
    46381 aatctctcag acaaatctgg tttttagact ttgatgggtg gaatagttag tttttttgat
    46441 aaactagaga ttttgcaaaa tctctttttg taatcatggg catacggttt taatttattc
    46501 ttttcaaaaa aaggttttct atgataagtg attttatcaa ttcttttcag aggtaatgaa
    46561 ttaagtggca aataaaacaa tcaaagtaga aaacgaagtt gtaaaagagt taaaagaagg
    46621 ggcagaagat ttagatattg taaagattgg agtcgatgtt ccgggtcttg tttcgatgat
    46681 caatgaaaac aatattcgta tgtatgatga tgaagaatat acgatgacag atgtcattaa
    46741 aagtgtcata gaaagtaggg catcactcag tagttttttc cttttagata taggagccat
    46801 atttagaaaa tataaactat ggatgcgaca tttgccaaat gtaaaaatgt tttatgcggt
    46861 aaaatgcaat tctgataaat tattactgca tactctagtt gacttaggag ttggatttga
    46921 tgttgcttct aaggtagaaa tttctactgt tcgtgagttc gatgtagatc cagacaaatt
    46981 aatttttgca aatcctgtga aagaaatcaa tcatattacc ttcgcggaag gggaagggat
    47041 taagaagatg acttttgata atgtggatga actaaaaaag atatctgttt ttcatccgaa
    47101 tgcggagctt gttcttagaa tccttgtaga tgattcaaaa tcaagaatgc catttggcac
    47161 aaaatttggg tgtcctacat ccaatctacc ggaagtattt gatttggcta agactctcgg
    47221 tctaaatatt attggcgtta gtttccatgt tggatctgaa tgtgaaagtc caactgctta
    47281 ttcagatgca attgctcttg caaaagaagt ttttaacaca gcaaagacct atggattttc
    47341 tcttagactt ttagatattg gtggagggtt tcctgggcat gattcccctg agatggaaaa
    47401 taaatttatc gagattgcgg gcgcgattaa cgcacaattg gcaacttctt tttcagatgt
    47461 gccagatcta gaagtaattg cagagccggg acgattcttt gctacaagct gcggaacact
    47521 tgttaccaat gtgattggtc gaaagattat cagcaattct gattcagaaa aaatcattca
    47581 ttactatatt aactccaatc tatacggagt ttttaataat attgtatttg ataaagcaag
    47641 acctgatttt gaactgagaa cccctgtggg ggaaaatgaa ccgctttata aatcagttat
    47701 ctttggacag acttgtgata gtatggataa gattgctgac ggaattatgt taccggaact
    47761 tgtgtgtgga aactggctga tcattcgcaa ccatggagcc tatacaattg cttccgcttc
    47821 taaattcaat ggattcgaat tagaagatgt aaattacgta tttacgtttt aattaaacgt
    47881 gagttcaatg gaaaaatctt ttcaccactg atgcacggtt cgactacgct caccgcattg
    47941 caccgataaa tcaatgagta agaagtgatg gataatttta aattatcgta tcttttatcg
    48001 gtgataattg tccctccacc caaaatccat taccgctcat tcgcggtaat aggtggtaat
    48061 cctttatttt ttctgttacg ctgaactgga gttaattgaa ttataggatt tttactgatc
    48121 ttgggaaagg cttagaaaat gaaccgatgc ttgatccgcg gggaaaatac tgattacccg
    48181 cgaaaaacat tcgattgctt tttgaaaatc tttgttccaa aaataatcta agcctttttc
    48241 gaaatcatct ttcgttttaa gatgtaaatc caatagatag tctggcattc cattgaaaat
    48301 ttcgtaaact gcaatcgtat ttgattttcc ttttactttc accctgtcca ggattctata
    48361 tttatatttt tcgggatttt ttaagttttt aaaagtatac tcactgatta aaatataagc
    48421 gccatagacc tttgtaagtc cttcaattct agaggcaaga ttgacagcat ccgaaatcac
    48481 tgtagtctct agtctttcct cttcgcctat cgtcccgaga atcataggac ctgtgtgaac
    48541 tccaattccc actctaatag aatcataatt ataattcata cgatgttgat tgtagattcg
    48601 aatcttttcc tgcatttcta ttgcagcatt taaagcatct tccacattcc ccggaaaaag
    48661 agccatgatg gcatctccaa tatatttatc tataaaacca ttgtgacttc gaacgatagg
    48721 acccatacga ctgagataag aatttaaaaa attaaaatta tcacttggac tcatcttctc
    48781 ggaaagttct gtaaaagagc ggatatcact aaataaaata gacatatctc tttgcacttg
    48841 ctcgcctaag cgaatttcag ttatgttttg tttatctaaa aagtttaaga attcgatggg
    48901 aacaaatcta ctataggctt tatttacttc ttttagatcg gttgttaaat tttcgataga
    48961 tcgaaatgct tttgaaaatc gaatggagat tcctagagac tgactaaaaa ttaaacaggc
    49021 tagtccataa tgggataaaa agaagctatt gatccactga tttgcataaa gtaaatcatt
    49081 gactgtcgta agaatcataa atacaaagcc gaagagtata agctttgctc ctggtaagtt
    49141 ttgatataca tagtaaatca taaaactaaa tcccaataat ataaatccaa agtaaaaagg
    49201 ctgatacaag ggaacagaat ttgtaaaaaa atagggtggt aagaatagac cgattgataa
    49261 taaaattgag atacagtaac aaatgatcgt taggcgtttt agatagggtc tgttaaactg
    49321 gctgtataaa aaatgatatc ctaatggtag agtcgcatac caagtaaaat actctactcg
    49381 aatcaataaa ccaaacggca cttgaatata ggaattaaaa actcgctcac caatggatag
    49441 agatcgaagt aaaattacaa aacagaagac agaaaataaa atatttgaga tatcggatct
    49501 tctgtaaaaa tagagaaata aatgatagag tatcatgata aaaataatac cagttagaaa
    49561 aaaatccata tctctaaaaa aatttgtttc actgataatc tgttcatggg ttccaagtat
    49621 gaggctatac caagctccac catttctatg ttcataattt gaaatccaat aaataatctc
    49681 tacatcctct tctggattcg aaagaaaagc ctgggtattt ttataatctg gaatcgaatt
    49741 ttcaggagaa gtgcctactt ttccggcaga gagt
//
LOCUS       DAOQOS010000031        49458 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_274_length_49458_cov_6.079449, whole genome shotgun sequence.
ACCESSION   DAOQOS010000031 DAOQOS010000000
VERSION     DAOQOS010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 49458)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 49458)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49458
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_274_length_49458_cov_6.079449"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            288..470
                     /locus_tag="PLF66_08780"
     CDS             288..470
                     /locus_tag="PLF66_08780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64173.1"
                     /translation="MSLQAYKFEAIVFQDGIIHIQQMLELANQDIEFFILVKHKPSNQ
                     NKPQEVDLFYKNGQAP"
     gene            complement(586..1155)
                     /locus_tag="PLF66_08785"
     CDS             complement(586..1155)
                     /locus_tag="PLF66_08785"
                     /inference="COORDINATES: protein motif:HMM:NF020052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification enzyme R subunit
                     C-terminal domain-containing protein"
                     /protein_id="HMZ64174.1"
                     /translation="MYQIAPSRVREIKHTVTTSFWSADGKPITAEEFLKHLLGELPNF
                     FTSEEELRILWSNPITRQTLLENLEDAGFGIQELTTLQQLIEVEKSDLFDVLEYVFNS
                     NTKPITREERVLNAQKNIYASLTKEQIEFVDFVLSRYIETGVEELSQEKLPILLKNKY
                     QSLEDAKSILGEVKEISSLFINFQKFLYT"
     gene            1393..2565
                     /locus_tag="PLF66_08790"
     CDS             1393..2565
                     /locus_tag="PLF66_08790"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HMZ64175.1"
                     /translation="MWIERDISQTINKSKDVIQVIRGPRQIGKTSLFLKLDPDFKELS
                     LDDPILRELAKNDPDLFLKQFSDKKIFIDEAQYAPSLFPSLKRKADLYKRENKGRLHT
                     IIRLSGSNQILMDKNVKESLSGRAGFFDMNTLSVSEILAFKKYKIQDILFRGGWPEIY
                     AEKNIDPKKYLDDYINSYVEKDIVLSAGIQKSNQFLKFIKLLAGRVGNLLEYASISQE
                     VGVDSKTIKDWVSVLERMQIISLLMPYSTNLTNRLVKSPKIYFLDTGLACRLQGWTSA
                     EPILTSPQQGGLFENLVFSEIYKLNLNYQLGWQFFHWRSRDGEEIDFLIQKDPIHFLF
                     IESKVSPQNLPPLTDFREVKKVFKEKIPKIILCHQEGNYILNEKVPISLLKEFLMK"
     gene            complement(2608..3567)
                     /locus_tag="PLF66_08795"
     CDS             complement(2608..3567)
                     /locus_tag="PLF66_08795"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HMZ64176.1"
                     /translation="MRYIGIDVAKDTFEVAFPSSNSKYKVVQFVNNEEGVKKFIKKLK
                     KDDRSVLEATGSYSTRLVKRIMQSGFEVSVVNPLRIKHFSKMNLSRTKTDSSDAVLIA
                     EFGLKMTPEPEKPSPEYMEDLSQRRSVLKQLKETVSRFKNQLHALKSRVRPDLTSIKF
                     IEETIKNLEKQIDELEKNMSEIVKEKASETYKCLKSIPGIGSLSAIELIIVTKNFENF
                     TSYKQLSAYIGLCPRITESGTSVKGKSSIAKMGMSYTRQLLYMCSLSAIKRNYVCREM
                     YDRLRKNGKHTMVALMAVANKLLKIAFTIVKNGDLYDEKVCLA"
     gene            complement(3894..4988)
                     /locus_tag="PLF66_08800"
     CDS             complement(3894..4988)
                     /locus_tag="PLF66_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004769978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 N-terminal domain-containing protein"
                     /protein_id="HMZ64177.1"
                     /translation="MIQMATESSGISQTQYHNVLKDISKIYEQSLEEGNEDWNKAVLS
                     SNWKIGQRIIEITQKKKARANYGDQVLKQLSKDLSRKYQKGFSERNLGYMRKFYLYYK
                     AKKFHSELSWSHYVSLLLIENQNTRLSLEKKAIQRKLSNRDLLSLVKQELADKKNKSK
                     PEPKTKEEAKTLTRPYLKLYTYKVVRKYKAEDADSIPHIDLGFSILLEEKEKLSKFKL
                     GSIVESIKDNKDYSFKLKTNHKERFTYKAYLERVIDGDTLLVTIDLGFSISIKQRLRL
                     RGLDAPEISTSPQNPLTSSKKGLASKRFIESCLKDCKFLIIKTHGKDKYDRYLVDVFY
                     LKNELDEEKVLKDGLFLNNELLAGKYAVRF"
     gene            complement(5707..8403)
                     /locus_tag="PLF66_08805"
     CDS             complement(5707..8403)
                     /locus_tag="PLF66_08805"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HMZ64178.1"
                     /translation="MIFASDLLQDYPLGYALLQNELNQSRSEFTFLEANIFFLRVFEL
                     DIKEILGKKIREIESTIVNANENWKQFLIFLSENKNQQTQIYQKNLNKWYDVCVQVST
                     KNQLIILTQDITIQKELELKVEKLNQENQQIKKLEKNFRTNEDKLQSLYELSPLGIAL
                     TDMNGKYIEFNSAFRDICGYTESELNQLDYWQLTPKKYESEEEKQLESLTKTGRYGPY
                     EKEYIQKDGSLIPINLNGILIKDAAGNSYIWSIVEDISEKKKTMEELHKNTALITSLL
                     DSIPDLIFFKDIDGIYLGCNPEFAKFVGKKREEIIGKTDYDLFSKELADFFREHDNKM
                     MQQLNARRNEEWVTYPDGKKILLDTLKTPYLGPEGELIGILGISRDITERKLAEDAIK
                     MSEAKWKSYIQMAPYAIFITDLQGNYLEVNPAASESTGYTQTEFLSMNITNLVSAEKR
                     EQEIFRFQKLISEGKLEAELELITKQGETKFFLLSAILIEGGKVLGVTIDITDMKKVE
                     RELIESKRLADTANRAKSEFLANMSHEIRTPLNGVIGFSELLAQTTLDETQNNFISNI
                     ISSGKSLLGVISDILDLSKIESGKLELDNTESNLVELMEEAVNIIKLQANEKNLELIV
                     DIPFDIPTVVKTDAIRLKQIFINLLNNAVKFTEKGKVEFKIVFEDLGEKKGAYTFSVI
                     DTGIGIKPEQMAKLFKAFTQADSSTTRKYGGTGLGLRISHLLVEKMGGQIEVRSEIGK
                     GSTFQFTLEIDFIPKPIKNQSSNIGHESKELSFVEYSILIAEDVEVNLLLIKALLKMY
                     FPNAKIYEAENGTEAIEAVEKHDFDLILMDIQMPGLDGNDATKRIREMEKTKAKRVPI
                     LALTAEAFKEEKEKALASGMDGFLTKPLDKNKLLSILNHFLK"
     gene            8958..10826
                     /locus_tag="PLF66_08810"
     CDS             8958..10826
                     /locus_tag="PLF66_08810"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HMZ64179.1"
                     /translation="MPKRLVPKSLTSEKRENHLTTYLRDFQKRGDFKKYVFVSLILLV
                     LFEILIGIGFNLIFSETLEENRKENYTRAWNNYLNQKWKSDHSKITGYVWWSDIWKSM
                     YKGDLKQLDTAFQADASFKENYDLFLVFTNPNSDPAFKIVGKNLKSFDSVDNNLIQAF
                     FREKNLKPGNIHTIEKLKDNRYFLISVSALCDDLGNPIFPGIAFFASDLEKFLRLAEE
                     VIPVKMEVKSGLPPKDIYHFFNMGNTLTNSGNFYIEVKPEFEIKTLVIKTLIFFISIQ
                     LILTISLFVLIVPRYTRRKTEKLKEIINASEALNYELMNKVEELKIAKEEIEKSEAKY
                     SHLIESSKDIIFSFDHNGVILTANKALVEFLGFKKEDLLGKYFLDLAYRPDKKLDSLE
                     KQLLMEKFEELKENKSSVSFDMTFGTKNNEPLQLGVKWEYVKIGENFVVFGKAFTVSE
                     DSMLKYVESENRQYVFNNYITLAEQVSQRITANLIKHLDSNDVFNIRICVREIIINSI
                     EHGNLNIDYNTKTELRKVKGRYFQFLQERQNDPLYKDRKVTVFYSLKSNRVSFLVKDE
                     GKGFDHKKMLADNADTANANFLDHGRGIYMTKNTFDKIRYNQAGNKVLLVKYFKKD"
     gene            complement(10880..12337)
                     /locus_tag="PLF66_08815"
     CDS             complement(10880..12337)
                     /locus_tag="PLF66_08815"
                     /inference="COORDINATES: protein motif:HMM:NF013595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64180.1"
                     /translation="MQVHIIKLFYFFLISILYSIYFLLFLNCLKAKKSPFDMRNSSTT
                     SIVASLSNRPTVSNTTTSSPQSGNISLGARTYTQVTINLTPPSGMNGTINYRIYASYP
                     DEWTTIDQVEANAVLKLDYTTNISAYTISGLEPYQFYRVDIIAKDSNGNKLLYTPFCI
                     QMDPNISGTVTCSTGPKGIVTTLTGTGGLGSADGSFSTSTFNQPQGIVALGNSLYVVD
                     TSNNRIRKLDMNNQTVSTIAGSTLGYTNAIGLSAQFNSPTGITTDGTNLFIVDRNNYS
                     IRLLNLSNLTVTTFAGPATATLGYTDGTGSTARFNAANYIIYNGSDFYLSDEDNCRIR
                     KITTSALVTTHAGSTCPGTNNGLLLSASFSQDIVGITFYQSYIFLTDKGNNNIRKIDT
                     SLSGSVSTFTNMTDPHATVVYGKYLYVSENTGHRIYKYDIDTGIGSVFVGSGSSGSSD
                     GTGTNASFNLPRAMTFIGNVLYVADTNNHKIRKIE"
     gene            complement(12746..15916)
                     /locus_tag="PLF66_08820"
     CDS             complement(12746..15916)
                     /locus_tag="PLF66_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004496544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HMZ64181.1"
                     /translation="MNIAKLSINRPIFISSIVILIIFTGWFALNRLGVDLFPDVNIPV
                     ISVSTVYRGAGPEEVETLISRPIEEEIGSISGLKRISSRNQEGISIVIAEFFLTADIK
                     DSEQQVRNKIARIRRELPSDIDEPVIQRFDPADQPIVRLSLFGEIPPVELYDIAKEEL
                     KPGIEQVNNVGSVRIIGGAKRQIQIEVDRKKLNEYSLSNVFMGNQLKSFGLNVPVGKF
                     ESGKSEVSYRTIGRFEKLSEIEEAQVFFGGELGSSVPVSKIARVVDTIEDLKTMCFLY
                     APTKEDPPTHSLIQGFINSIRKGDPPQIERLTKPSLFIDVYKQSGANTVAVVDSVLQK
                     VEKLNETVLKDRKGNPHIIVVRDSSIWIRANVEDVSVSLILGILLTVIVVYFFLGNVR
                     STIITGLALPNSLLGAFIIMYAMGFTINVMTLLALSLTVGLLVDDAIVVRENIFRKLE
                     EGHPPVIAAEIGTTEVTLAVVATSLTVIAVFLPIGFLSGIVGQFFKQFGLTVVFAMIV
                     SLFDALTIAPMLSAYFAGKAHGNPNFLVKIFDNFQTWLEKVYGIILDFVIKRPIIVLI
                     AATFVFVFSIYSLKFVKKTFLPPNDQGEFLVSIDMPPGTSLQGTYDVIQKIEEKLKTI
                     PELNKLATVVGSPDGFPTIGNIGIALVPSHQRRRSTAVVKSEVREMMKEFNYARPSVN
                     DYSAVGGGVNYPFNLNIMGDDLEAIEKYSKELIEKLKVIPDLTDIERDYQAGKPEFQI
                     EFDFKKMQSVGVAPITAGAELRYHIEGGVVGKLHDKGLEYDILMRLKPEQRNLRSAYL
                     DTKVPNVNFKMIPLNFVSSGKEKLGPAKISRQDRSRVIPIRANLTSTGAIGNATDMAK
                     KILAETFPPPPGIRYAFYGQSEDFRELMQNIIIAFGFALLFIYLVLASLYESFITPIS
                     VLIAIPPAFSGAFLALALFGEMLNLFSMIGIILLMGLVTKNSILIVDYAMRAIHSGVD
                     RKEAIKTAGVLRLRPILMTSLAMIAGTLPIALGLGEAAKSRTAMGIAIIGGLILSTLL
                     TLIVVPALFGYVDRLRESIESKFRHS"
     gene            complement(16096..16596)
                     /locus_tag="PLF66_08825"
     CDS             complement(16096..16596)
                     /locus_tag="PLF66_08825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64182.1"
                     /translation="MKQIIILLLLFMSLGILSKESTSSDTGKKLGVGISLFSPTGITG
                     KYWLDDKKTLEGTLGIGYQEKWGQYLHFVFLYNFAEIKPSLYVYAGGGISFSERYEKL
                     NPGRGRFVERDHYFFSPGLRIPIGITYMNREKNFEIYGELAINIYLSDRSGFGPGIAI
                     GARYYF"
     gene            complement(16596..>17721)
                     /locus_tag="PLF66_08830"
     CDS             complement(16596..>17721)
                     /locus_tag="PLF66_08830"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HMZ64183.1"
                     /translation="IKDTIIQNYQSFLYRSVIGALGGNSYANSIVPTALRGKKSFKKI
                     RKQFFYFRECLFEKDGFMFYRFLKIYLLTLLFYIPAFAESAPNELGKFLIITYHVIGE
                     KDSAYTRTIQGFKDDLALLKKGNYYPLKISDLEKRNLNIPKGKRPVFITLDDSSQSQF
                     DMDEKGSISPNCAVGIMEDFKKKNPDFPLTATFFILPGAKHPNNLFGQEKLTSKKLEF
                     LTKNNYEIQNHTLWHANLKKYKDKIGEQIVESQKLLNKYLPDYKMTSLAMPFGIYPPK
                     DYESKLVSGSYKGIEYKHILVFDYSNRPSYSPYDKEFDSIHVRRVQAFKDNIEKMIEK
                     YSESPDSYVSDGDPNTITFPRKLEDKLNLKFKEKLKVVTY"
     assembly_gap    17722..17821
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<17822..18516)
                     /locus_tag="PLF66_08835"
     CDS             complement(<17822..18516)
                     /locus_tag="PLF66_08835"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HMZ64184.1"
                     /translation="MKKISILFLLLPLCFLLSQDKQKETLPAGFIWPVGGENLEVKLS
                     SLFGESRGDHFHNGVDVSSDNEKVSSIEKGTVIYSHYNSDNPFENESGSGNSVWVLHD
                     NDILSAYYHLKDGRSLEVLEKKVLEKGDSVGKTGNTGHSSGSHLHFVVATDKGRKIID
                     PLRILPKVKDTRPPSIGSLILTIGKNMTYINDGDSFNSSSNFPITVDISDAGERKGQR
                     RGVKTLRFSLNNK"
     gene            18584..19486
                     /locus_tag="PLF66_08840"
     CDS             18584..19486
                     /locus_tag="PLF66_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011672080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HMZ64185.1"
                     /translation="MKLSQEEVFSLVQSCASGNEASLKKFFEEFAEDIYNFPIRVFHL
                     DEDDASEFFIYAYERLKSGKRFHTFVGKSGFKTWLYTVLRNMLIDWRRNKRELKIVSN
                     VKVNSDGVEYYSIENEPDLKSNLQKEADSVSSRFQDILSEIKVESRIIFKLAYIYYLN
                     LNDEEIDFLVTRTGKTKEQIQKDILNIRENLSEKEIQNLQSEDKIVSIYMNILELKEQ
                     QEKEKPIVFEDNLPNYDKVQIAINKKYEQRRRLIVKKSKGNFITRTPYRVIAELLKIP
                     EGGVSISLQRVLEKIQKKFPKHEI"
     gene            19525..20265
                     /locus_tag="PLF66_08845"
     CDS             19525..20265
                     /locus_tag="PLF66_08845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64186.1"
                     /translation="MNSKNNQNDQPNSKKYLDLFLNEGQLHESALENDTDFQELIQLE
                     ESIVNTIQLENEKHLDHILEKDSSFYEDLDIAKANLNITPDRNRPLPLYLKNYMEADF
                     AAQKKEEDRLVIRFGKAGVKLLGSFFGQTEISPLELSIPSVRNSINTTPQSISMLEKT
                     DQGNLVYQVIQENENEAYLSVKFDPSFVAIYNQVNLRKDNRFIYSSNINSEGIVSFSG
                     LKEGVYNIEFTGKNVSKRFDLSLFADKL"
     gene            20269..22080
                     /locus_tag="PLF66_08850"
     CDS             20269..22080
                     /locus_tag="PLF66_08850"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HMZ64187.1"
                     /translation="MIDLLIDRVGNVNIFNFLNAGQFHSESHIQSSIDTDLISEYVKE
                     VENISKLSNNFIANHESNFRVKPNVLKDLKVLGETFYEQFFPSSISDNLRSTSEKYLH
                     FNIDSSLAEIPWELLYDGECFLSDRFLIGRTIKGSKTFPQEENNEKLKMLIIADPTED
                     LEWAQKEGEVLFQVLKEKVPASVLDLEFIAGSQITKLKLLSMIRGKNIIHYAGHLFFS
                     NDPMENGWLLSGDKILKAREIKNSAFSTNLVFSNSCNSSRSVGIKNSKSIINDFASSF
                     LMSGIMSFIGTKWEVADNSTTLDFTIRFYLALFNDKSLGESLHIAREYARKNSDPLDL
                     IWGNYSLYGHPDRIILNKNNNRRNKIIEPMMIRKTYPTPIAISYSNFISIERMDSTEN
                     KMKAFVNSFEEFTRLITIIVISDHKYKALNQNFENKEDLSLEYLWDIIFKSMLDFKKL
                     QISMVVESFLVVLFSNKDLIYKIISWIQKFRAGGLDTESLEGYLITSQYYYENLLVEL
                     NEFEKCYIFYISNDSDRHCLFYGYNKKETEIDATQFGFFLNRLEEFRGRVILIHKTKK
                     SILPLIGIQIKYKNDERDKDLSLDFMNFRDNIVPRLQ"
     gene            22167..22772
                     /locus_tag="PLF66_08855"
     CDS             22167..22772
                     /locus_tag="PLF66_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016758843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64188.1"
                     /translation="MKERTEYFEDTVDYSRRETVISFREVFEKKESTYPKKDHTIYNC
                     PFLGYINKDFTRIGCMIHPIRTGDPKSQNFSFYGSSICQTYDCRNKDRKNGMDWEDYF
                     NSLKLDFYDYSHLASDHILITRLESFFDSLQIPLKDIFLKHSPLLKRIFQYKVNQMNH
                     LTSFEIDMNSNTGTPFERLIEILSLEPDHPLYLELKLFCKE"
     gene            complement(22834..23106)
                     /locus_tag="PLF66_08860"
     CDS             complement(22834..23106)
                     /locus_tag="PLF66_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008595105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64189.1"
                     /translation="MEKEIKDALNFVIGAATTVKSETEKLVAGLETEFKKLADKGAAD
                     NSEAAMNVRKYTEEAFREVEKVLNDAKAKFEEAKTQVKNLVPEKKA"
     gene            complement(23192..25441)
                     /locus_tag="PLF66_08865"
     CDS             complement(23192..25441)
                     /locus_tag="PLF66_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004433066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HMZ64190.1"
                     /translation="MKMSKIKGKTILIIGGGLLQVPLIETAKSMKLRVVVADMNPESL
                     GFKLADEKIIMSTKDIEGMVRESKRFSEKEPVHAVITAGTDASMTVAAVANALGLPGI
                     RYVDAEAASNKVKMRKRLKEFNVPIPNFAPIWTIQDARDALDYLKFPLVIKPSDNMGA
                     RGVIKVNNRDELHSAFKHAKKYSPTGEVILEEFMVGPEVSVDALTWNGNIKITGIADR
                     IIEREPYFIEIGHNMPSKFSKEVLDEVEDVMKKGMRALGITLGAGKGDIKITPDGVKI
                     GEIAARLSGGFMSAYTYPLSTGINLYRSAILIAFGEEPDDLEPKLNLVSIERSLLAAP
                     GKIISISGIEEAKEIEGVNEIFLQSKVGDIITDPTNNIEKSGHIIITAETLEKAEKIF
                     EEAKQIIHFESDSYYSLTEKSINQTARNKFGKEICWVCKVCDGTDCASGVPGMGGVGR
                     METFKDNSIALSEYKILPRYIRESVSPRIEWNFLGRSFQSPLMAAPMTGATTNMNNAM
                     DEYQYSVAVLKGCNESGTIAWLGDGATPDKYKKMLDAIRQVNGNGILICKPRADESLL
                     EERFKEAEAQNIFALGMDIDAFNFKTMILKNQQSPSRGLKELTRIRSMTKLPFILKGI
                     MTVEDAILAIDAGSDAIVVSNHGGRVLDEMPGTARVLEKIAEAVQGRIPVLVDGGIRS
                     GMDIFKMIALGAQAVLVGRPIAISAVGGDFAGVKYLIHQYTTDLKNTMNIAGAGSLSS
                     ISKTMLLKK"
     gene            complement(25445..26587)
                     /gene="mnmA"
                     /locus_tag="PLF66_08870"
     CDS             complement(25445..26587)
                     /gene="mnmA"
                     /locus_tag="PLF66_08870"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017854419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HMZ64191.1"
                     /translation="MSQKEKIIVAMSGGVDSAVTAGLLLEKGYDVIGVNIKTWEYEAP
                     NCDTTKKSCCSPEDIRDARDVGLSFNIPFYVIKMEKIFGDKVIDRFISDYQNGMTPNP
                     CVECNTFVKFGSLFEKGKVLGIDKIATGHYANIHVAENGRYAIANGVDTKKNQAYYLY
                     GLSQENLKNTVFPLGNMTKPEVREHARRFGLSIAEKAESQEICFIPENDYRSFLKKKN
                     VDFTPGFFKLSNGNIVGKHSGKEGFTVGQRKGLGVSWKNPLYVLSIEEDGTVILGEEE
                     ETYCEEFIVTDVNYQGLTPLNEGEELTCKVQIRYRHRPVQCEVKKEKNKIIVKPLELV
                     QSVTPGQSSVFYPTNGDYLLMGGIIEKGSIKLKKIKTSIVNDLNLV"
     gene            complement(26587..27507)
                     /locus_tag="PLF66_08875"
     CDS             complement(26587..27507)
                     /locus_tag="PLF66_08875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64192.1"
                     /translation="MIFKIKLLYRPNLFGRIIAIFLFLACRTTNSEFIKHLPHHEFQA
                     NQSNLLQPRIEIFTKFQQEPIKKLTFILDTGSNVSLIRNSLYKAVGDSNKFKLKSISG
                     EIEGDFGEVKLNLFDSSERIIGENISFHTFEFDSRFAFDGLIGNDVLSKFSLFLELPE
                     NVYIIQPNIKTKKFEGFTKIPFHFADGHILVHALIENRLCKLLLDTGAGISYLNQSKA
                     AELNLKRGGKASFIDLRGEEQNTFYHIGENLCVAENLCQKKIELLSGNSIQDFLSKEG
                     NLDGILGMNWVENYSILIDYQNLELYIKKR"
     gene            complement(27512..28579)
                     /locus_tag="PLF66_08880"
     CDS             complement(27512..28579)
                     /locus_tag="PLF66_08880"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002726390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HMZ64193.1"
                     /translation="MSKKKEDSGSFEQLEHYEKKIYDQKQLLEISKALNSTLDYNYLI
                     DAILNICLAQLQTLNAAIYLAPDVDADHFTLDSSHKGFDIADRDMKMKIMIDSPLVQF
                     FEEKPKAMTIRQLTDNGFADDELVKYFKALGAEMIIPLNAKGKVNGLLMLGEKMTMSD
                     FLESEKEFLTILASLAGVAVENSRLYELSTVDMMTQLKIHHYFQSKLREEMDRCRKKG
                     TKLALLFTDIDKFKVFNDTHGHQAGDVVLIEVAQKLITCARKNDIPARYGGEEFCVVM
                     PGADLQEGYEMGEKIRKAVEAQVVENPNGGDPLRVTISVGVTEFYPTDKNNKELIERA
                     DKALYQAKHGGRNQTVCFKVQ"
     gene            28734..28806
                     /locus_tag="PLF66_08885"
     tRNA            28734..28806
                     /locus_tag="PLF66_08885"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:28767..28769,aa:Met,seq:cat)
     gene            complement(28984..29760)
                     /gene="proC"
                     /locus_tag="PLF66_08890"
     CDS             complement(28984..29760)
                     /gene="proC"
                     /locus_tag="PLF66_08890"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002189049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HMZ64194.1"
                     /translation="MKKVGIIGLGNMGYPIYKAIESDFSVLCYDPYSKRNDIKFESDF
                     SSLNDSCDRLILCVKPDKIVDTLKQIKEPKKIFSIAAGINLDTLKKHAPSNSYVVRIM
                     PNLPLKIQEGCMGYIGDKEAYPDIKEIFNKLGLLVELSSELAIDAVTGLSGSGPAFVF
                     SFIQALAEGGVKSGLTYADALRLSIQTVKGSAMLLEKELEKENTHPSVLRNRVTSPGG
                     TTIFGLEKLEENGMHYSVMNAVYSAFKRAQELSQPHQNLK"
     gene            complement(29757..30428)
                     /locus_tag="PLF66_08895"
     CDS             complement(29757..30428)
                     /locus_tag="PLF66_08895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000538447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HMZ64195.1"
                     /translation="MLIRENYDFILDQIQKVRPENTPSLIAVSKKQPIEKITNALEKG
                     IRIFGENQIKEGIDKFQGLTGIYPDLELHHIGPVQTGTIRKLLGFFSYTHGVGTKNSL
                     QELIKQANQRKAKIYYFLQANLTLEDTKSGFIRKDLIEILRDLSALESEFAHFHGLMT
                     MGPSSGELITTRKVFQELNQIRKDYAPNAKLSMGMSGDFLIAAEEGSDYIRVGSAIFG
                     ERILK"
     gene            complement(30428..31087)
                     /locus_tag="PLF66_08900"
     CDS             complement(30428..31087)
                     /locus_tag="PLF66_08900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64196.1"
                     /translation="MSKQNSLKIILTTIIVLIISAIAVFTVVNWAEVVKKFQGAANKD
                     GFEKHERNTVPPVQNSTTSNTVDESKNSSLVPDETKTTPHPTETAKTTSKFDEPLSPP
                     VKDTTPIGLDEPPIIKPKDSAKTKINEDNFATNVLIDSPKTNFNSEKPPKISPKKHKH
                     GRKHHHKKNKARNSVKTSGLEKRVSYLEKKFGIKKPKSKQASLANRVYRLEKMITKSK
                     K"
     gene            31460..32200
                     /locus_tag="PLF66_08905"
     CDS             31460..32200
                     /locus_tag="PLF66_08905"
                     /inference="COORDINATES: protein motif:HMM:NF016501.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament outer layer protein FlaA"
                     /protein_id="HMZ64197.1"
                     /translation="MRKFTMKSLILFKIIFLLGFIFQNSIDSESEDMNRIKLIQDAVA
                     KGDRYKIHPVELFDGEMPWRVVKGTSFLDKTSFVSKSPNTEEFALESKFYYPLKTPEK
                     LKSLQIYSNIEIPGRDKYFISPEIETDFPVGIPSRIFLWVYSNNYDMSLKVILSKKRA
                     KDIIVDFGSLRFNGWRRLDAKVNLDRKMERLNLSKKSVYSIKGILVETSPSQSKGSFF
                     LFIDQMGVLLETPELYPGSEVPDGWELY"
     gene            32271..32915
                     /locus_tag="PLF66_08910"
     CDS             32271..32915
                     /locus_tag="PLF66_08910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64198.1"
                     /translation="MSKLFSEEELEQIKSAVKDAEKTTSAEIVPVFYESCGLYPDTYW
                     KSGIFFAAAYSFCYLFYTKLTNTWGDSLSKFFVMQMTSGLIGVAAVYLIPAWKRLLTD
                     KMFVKQRVRDVAYRVFLEESVFNTKTRTGMLLFISFFEKEALILGDEGINQKVTPDVW
                     EGILSQLIQGMKSGEKTNSIIAAIRSMGNLLKQYPIDAKDTNELRDDLRLGDTK"
     gene            32912..33889
                     /locus_tag="PLF66_08915"
     CDS             32912..33889
                     /locus_tag="PLF66_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015676197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HMZ64199.1"
                     /translation="MKKFSIIIFLLLQTASIFALDVPYLSGRVVDEAGILSEETKSSL
                     EKKLIEQEKKTSNQLVVLTIPSLEGEVLEDYSIKVATTWKLGQKGKDNGVLLLIAKDD
                     RKLRIEVGYGLEGTLTDVMCSRIIRNEITPSFKRGDFSGGVEKGVDSILAVIDGSYVN
                     TSSDSTTSNDSGKDTNIEGVYSKFIHSVGDSDIPFLFRIPFGLIFFTVITPFTILTAV
                     TPYVGWFLYFFLMPFYGTFPVVIFGKWGGLFLPSYIVIMFFLKLYFGFTKDGKKLAEK
                     IEQKYGGSGGSGGSSSGSSWSSRSSGGFSSGGFSGGGGSFGGGGSSGSW"
     gene            complement(34030..35730)
                     /locus_tag="PLF66_08920"
     CDS             complement(34030..35730)
                     /locus_tag="PLF66_08920"
                     /inference="COORDINATES: protein motif:HMM:NF024909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HMZ64200.1"
                     /translation="MITYEYSEYFPDDDKNFDKEKLMSILSDLIMKYDISLEEALRLM
                     IEKGIPANLFLKEGGMEDLIADFQKKIQAMIDQILKTFNLNPASEQLEKDLKSLRSDL
                     EKKFKKEENILQKLKAAIDGKNPDLLRRLKWDLAAQKKIEPRLDELIQKLQDIDKISN
                     GIKQYNFTGKKVPTPKEAGNILDNLDELTQLNRALEEAIQSGDLYNFNLEQLAKFLGP
                     ESYNEFIERRERIFEALKKLLQESGDIQENAEGGIELSPKSIRKIGKTALIEIFSKLK
                     TDSSSGSHVTNEFGDTENLTSSTKKLEFGDNVSNIDFPGSIINSILRGNGTIPSLGDL
                     DVFQARGNAKSSTVILLDMSGSMARSMRFYNAKKVTMAMDSLIRNEYKDEKLTVVGFG
                     STAKIFPITKVPTLQPYPVTIFNPYIKLKFDFAKMKKEDIENRVPQYFTNLQKGLQIA
                     RQALSSKQTKNKQIFLITDGAPTAHFKGSILHINYPPAPSDFEEALSEVKQCTEDGII
                     INTFLLTSEWDMNYFGEKSFIQQFAKMSQGRIFYPHPNELNQMIIYDFVSNKKKKFSY
                     "
     gene            complement(35727..36107)
                     /locus_tag="PLF66_08925"
     CDS             complement(35727..36107)
                     /locus_tag="PLF66_08925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64201.1"
                     /translation="MKKIILFLFISIQVMAMDHPIKLQLDKKSGDSYIMRVSVPKGYA
                     IQKDAPNKIKLSSEDKIKINPTELTFKGNTILGKPEYFEKVEDNTLTLNGKGKLQVSA
                     KIFYCDLNKNVCYPASIKQEETIQ"
     gene            36718..38127
                     /locus_tag="PLF66_08930"
     CDS             36718..38127
                     /locus_tag="PLF66_08930"
                     /inference="COORDINATES: protein motif:HMM:NF019237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HMZ64202.1"
                     /translation="MLLIRNILYLVLSILLFHCSESEYKNPCDPTSQNFLKLLLFKRF
                     TSDTASLCGLNTSNLFGTNSLSNFSYAPDLYYANGASISLSPTVSSSGLTFSISPSLP
                     GGISLNTSTGKITGSYVGYGGSSFTYTVQASNSSGSISTSFKLSIFGKNPIQTMQSQC
                     WNVSGTLDASCTAGTSIGQDGQLKKGTTPSFTGPSLVGSTDYITTDNNTGLIWKSCFE
                     GRSGATCIGTLSSLNATTAASTCTSLNSGLGYANRTDWRLASFGETNTILNHIGTSPA
                     TFTSNFPSASGGGTWSSTGDVSNSANAWYISFTDGTIGSTVKTNTNDVHCVSGSSLPA
                     AQFVDNGDDTIVDINTGLIWQKCTNGLSGSSCQTGTATQMNWASALTVCNGLSLNGKT
                     WRLPNINELKTIIDVTTSSPSTNTTFFPVTQLGNYWTSSTYTTTSAAWVVPFGASTFY
                     ATNLSKSSNAYTRCVTTGP"
     gene            complement(38124..39047)
                     /locus_tag="PLF66_08935"
     CDS             complement(38124..39047)
                     /locus_tag="PLF66_08935"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HMZ64203.1"
                     /translation="MSLIALFKAKGKNGFGYDTTAEEVTKNISLSGKTILVTGCNSGL
                     GNETIRVLALRGAKIIGTARTKEKAIEALKEISGNSHIGLECELSNPTSVRNCISEIK
                     KQKLKLDVIICNAGIMALPSLEKAYGYELQFFTNHIGHFLLVTGLVGELTDEGRVVML
                     SSEAHRNAPKEGVDFENLAGEKYYSAWTFYGQSKFANLLFAKELAKRFVGTKKTAYAV
                     HPGVIKTNLARNMNPVLQAIFSLGEHLFLKSIPEGAATQCYAATNPNVISESGNYFAD
                     CNIATPRKDANNEDLAKKLWDVSEKIVAGLT"
     gene            39613..39741
                     /locus_tag="PLF66_08940"
     CDS             39613..39741
                     /locus_tag="PLF66_08940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64204.1"
                     /translation="MIDGKYTDKTPDEINTKEAELYAALKQAQKDFAERFKIEIQK"
     gene            complement(39790..40875)
                     /locus_tag="PLF66_08945"
     CDS             complement(39790..40875)
                     /locus_tag="PLF66_08945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64205.1"
                     /translation="MKEKNWIYNQSFDLVFILSPPFLSIGFIYLFSDLFLDTVKMPLW
                     IWVSFILCIDVSHVYSTLFRTYFNPKEFQENKTLLTLLPIGLFFLGVILHLIDSKLFW
                     RVLAYSAVFHFIRQQYGFLRFYSKDSFGFSKKLEAVLLYSSTLYPVIYWHANLPRNFH
                     WFVDGDFLIGLPLWVERISFYFYLGIIGIYLLKELKEIITKKYFSIGKNLLLLGTALS
                     WYFGIVYFNQDIVFTITNVVTHGIPYIALIWVYGNKEAHNTNSPLLLNRFSYKVFFTK
                     FTFPMFILTLFLFAYIEEGFWAGFIWREHLEFFKPFISLPTIRSKETLSILVTLLTLP
                     QATHYVLDGFIWKLRDRDSYWYTVLFK"
     gene            complement(40872..42485)
                     /locus_tag="PLF66_08950"
     CDS             complement(40872..42485)
                     /locus_tag="PLF66_08950"
                     /inference="COORDINATES: protein motif:HMM:NF024842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HMZ64206.1"
                     /translation="MHTRRKFLQYLGLGSIAVFSSFFGLKNFLQRRQYNFKIQISGAN
                     FKAGHLLRENIKSIPENEIETETVIVGGGISGLSAGWYFKKNKYEDFLILEMDKSVGG
                     NSKSGKNDVSEYPYGAHYVPIPSSEAKYVRELFTELGIIEGFKKDLPIYNEFYLCADP
                     HERLYFQGIWQEGLVPAHGIEEEDKKQYKEFFSLVEKLKNTKGKDGKPAFAIPMEESS
                     QDKEFLDLDKTSMFDFMQRRGLTSRYLNWYVNYSCRDDYGVSHKKVSAWAGLHYFASR
                     AGKAANADSQTVLTWPAGNGFLVQKLSEICKDKIRTNTLTYRIEKYENFYYVDTIHPE
                     TNSLIRYKSKNIIYASPRYTAKKVIKNYKDDVANTLDFTPWLIANITLKEKPTGLGSN
                     LSWDNVSFYSKSLGYIVANHQDLTLHRKETVVTYYLPLDEEEPKAERLKAYLRSKEDW
                     VDIILEDLEKMHAGITDTILEIDLWIWGHGMVSPGINFLWSEKREKLLKSFMGIEFAN
                     SDMSGISIFEEAQYRGCEAAKKILKQISI"
     gene            complement(42475..43971)
                     /locus_tag="PLF66_08955"
     CDS             complement(42475..43971)
                     /locus_tag="PLF66_08955"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011669475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HMZ64207.1"
                     /translation="MSYVLLFSVFIIATCGLVYELIAGSLASYLLGDSITQFSTIIGA
                     YLFSMGIGSFLSKYIRKNLIGIFVQVELLIGIVGGSSAALLFLAFEHVASFRILLYTD
                     VSITGMLVGLEIPLMMRILKTHFEFSDLVSKIFTFDYIGALFASILFPLLLVPHLGLV
                     RTAFLFGIFNVLTGIWTLYVFEKEIPFLKTLKLAMFTGLILLFIGFVYSNQIMSFAET
                     ASYPDKVIYSKYSKYQRIVLTRSKQDLRLFLNGNLQFSSKDEYRYHEALVHIGLASLD
                     KPKKVLVLGGGDGLAVREILKYDSVEEIVLVDLDPEITGLFSKQKALTALNENSLLSP
                     KVKIYNEDAFIWLKTNETKFDFIAVDFPDPSNYSVGKLYTNSFYKILKNALATNGVGV
                     IQSTSPYVAKKSFWCVNNTLVSVGLNTLPYHVYVPAFGDWGYILFSKDKISIPEKYPT
                     GLKYITPSIAKTLTIFSDDMIVESNEVNRLNNQILVRLFEEEWEEYAH"
     gene            complement(43977..44198)
                     /locus_tag="PLF66_08960"
     CDS             complement(43977..44198)
                     /locus_tag="PLF66_08960"
                     /inference="COORDINATES: protein motif:HMM:NF015927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HMZ64208.1"
                     /translation="MLTDLISIKNIINAFLYSGIGIIVLAISFIIFDRITPGNLWEEI
                     AQKNNMPLAVTTSAVTIAMAIIIAAAIHS"
     gene            complement(44214..44354)
                     /locus_tag="PLF66_08965"
     CDS             complement(44214..44354)
                     /locus_tag="PLF66_08965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64209.1"
                     /translation="MNSRNFLGYGIILCILLILGGYTQWNVSDLFSPGKWGPRGPNSY
                     HK"
     gene            complement(44351..45688)
                     /locus_tag="PLF66_08970"
     CDS             complement(44351..45688)
                     /locus_tag="PLF66_08970"
                     /inference="COORDINATES: protein motif:HMM:NF025158.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4178 domain-containing protein"
                     /protein_id="HMZ64210.1"
                     /translation="MEGTNTKSTRVKTFSCPSCGGSVNIRAIGATINVVCAYCSSIID
                     TSDENYKVVDAFTKKIQRRTIIPLGQRGTLFGTLWETIGYMERTDQEGYTWSEYLLFN
                     PLKGFRFLSEFNGHWSFVTMIKDHLKIRRSADPVYVPYNQRDYTLFNKGTATVTYVIG
                     EFYWQVRMGETVNLEEFISPPETLGVESTEEELVWSVGTYVNSEEIKTAFQLTEPMPL
                     LIGIAPNQPGPINAEEKKKIQKYWKWIFNAMILLQLGICIGFGTETVYKGEMDFTIED
                     KGKTKVTPQFEIKDRVTSIDINLSSPVSNSWVEISGDLVNDETGASVDFEKGIEYYSG
                     VDSDGSWSEGSTSTTADISGVKRGKYHINIEMTTPAMQTGAANPSNPLEATKLSIEVK
                     TGSTIWANFFLAEFLLCLYPFYVWFRSRSFENKRWANSNFSQDSSDDSEEEDE"
     gene            complement(45690..46316)
                     /locus_tag="PLF66_08975"
     CDS             complement(45690..46316)
                     /locus_tag="PLF66_08975"
                     /inference="COORDINATES: protein motif:HMM:NF025158.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4178 domain-containing protein"
                     /protein_id="HMZ64211.1"
                     /translation="MKASCPSCGAEVPFQSRFAFMAVCKYCKSTIVRHDKDLELLGKM
                     SDMPEDMSPIQIGTTGIVGKDPFEVIGRQKIGYSNGVWNEWYLSFKSGKDGWLSDAQG
                     FYMISFEERDFKDFPDKSELKVGNILEIKKTEYSVDDVHEVTCRGSEGELPFKCLVGR
                     KSLSVDLRSERKGFANFDYAEEEQHLFVGRYFEVEDLKLENLRQIEGW"
     gene            46896..48314
                     /locus_tag="PLF66_08980"
     CDS             46896..48314
                     /locus_tag="PLF66_08980"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HMZ64212.1"
                     /translation="MQEQNTTTEIKTPNTEMEILRKHFPHCFDKNGNFDLEKFRRGLS
                     DKEISFSTESYAMDWLGKSYARLLASDPATTLLKADETHNAKPENAKSENLLIKGDNL
                     EVLKHLVGAYYERIKMIYIDPPYNTGSDGFVYADDRKFTVPELARLAGIEEEKAKRIL
                     EFTQSKSNSHSAWLTFMYPRLYIAKQLLREDGVIFISIDDNEVAQLRLLMDEIFGEEN
                     FVANICWKKRSSPDARTVIGAIHDWLVCYKKIGENSRANIGKMPLNQERLDSYQNPDN
                     DSRGPWASVDMTGMTARAPKSQFYKITLPSGRVIGPPSGRSWGVAESTFYELISDNRI
                     WFGKSGDNVPRIKKFLSEGEGQVVPTFWDMSEVGSNDEAKKELNDILNTPEVFDTPKP
                     IRLLKRMIQIASTPNSIILDFFAGSGTTGDAVMQLNAADGGNRKYILVQIPEPIDPKK
                     NKIAYDFVKDLTSRGGGGTKYF"
     gene            48559..48918
                     /locus_tag="PLF66_08985"
     CDS             48559..48918
                     /locus_tag="PLF66_08985"
                     /inference="COORDINATES: protein motif:HMM:NF036754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64213.1"
                     /translation="MQDQKLFDESKLTNEDLQTLLITWKTYDGIPLTQNLEKISLGTY
                     TAYYGKGRLYLMHKGFQTDHLKVLLEKIDTDKTFEPRSIICFGYHFESKNLRELSENG
                     KSYANKKRLDIDFIIRY"
     gene            48927..>49458
                     /gene="rhuM"
                     /locus_tag="PLF66_08990"
     CDS             48927..>49458
                     /gene="rhuM"
                     /locus_tag="PLF66_08990"
                     /inference="COORDINATES: protein motif:HMM:NF024706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RhuM family protein"
                     /protein_id="HMZ64214.1"
                     /translation="MSTPSEIILFKTADEKVSIQVIMEDETVWLTQEQISQLFERDRS
                     VITKHIGKIFREGELDERVVCANFALTTPHGAIEGKTQEQEVKHYNLDVIISVGYRVK
                     SLRGTEFRKWATQRLKEYIIKGFTMDDERLKGAGGGNYWKELLERIRDIRSSEKVIYR
                     QVLDLYATSIDYNSKSK"
BASE COUNT        15404 a         8612 c         8449 g        16893 t
ORIGIN      
        1 atcaaaagcc tgatatcgtt agatgtcggg cttttttgtt tttgtgctgg tgtgagtaaa
       61 gcttgagaga ttacttgtaa aaaaaattcc agactgaatt tcgaaagacc tcatcgcctt
      121 tgttaaaaaa agctcgttgc atgatcttct gttgaactat cattacagga gacggaaggt
      181 ttaggactta gaaatattga ttccaaagtg catgcctgaa aactgcgatt gcagtaaaaa
      241 tattttttca ttgatttttt taaaaaaaga actattaatt aatatctatg agtttacaag
      301 cctataaatt cgaagcaatt gtattccaag atggtataat tcatatccaa cagatgctag
      361 aattggctaa tcaggatatt gaatttttta ttctagtaaa acacaaacca tcgaatcaaa
      421 ataaaccgca ggaagtcgat ttattctaca aaaatggaca ggctccttaa caacattgaa
      481 ataattctta ccagaaatga agaagattta aaagattctg aattaaaaat ttattcgcct
      541 gtgaattttt tgaataaggt aagagaatcg gaatcgtatt tatcgctacg tatacaaaaa
      601 cttttgaaaa tttataaata gcgaactgat ttcttttact tcccctaaaa tagatttagc
      661 atcttctaaa gattgatatt tattttttag tagtataggt aatttttcct gtgaaagttc
      721 ttctactcct gtttcgatgt atctactaag aacaaagtcc acaaattcaa tctgttcttt
      781 tgtgagagag gcataaatgt ttttctgtgc gttaagaacc ctttcttctc tagtgatagg
      841 ttttgtattg ctattaaaaa catattccaa aacatcaaat agatcagatt tctctacttc
      901 gattaattgt tgaagtgttg ttaactcttg aattccaaat ccagcatctt ccaaattttc
      961 caaaagagtt tgtcttgtga ttgggttact ccaaagtatt cgcagctctt cttcacttgt
     1021 aaaaaaatta ggaagctctc cgagtaaatg ttttaaaaat tcttctgccg taattggttt
     1081 tccatctgca ctccaaaaag aagtggtaac tgtgtgcttg atttctctta cccgagaagg
     1141 tgcaatttga tacatagagt ctttcgattc aagtcgaacc ggttctacgg gcgaaccgat
     1201 agattggata aatagattta aaatttcagc atgatccgtg atcttctttt tttaccaccg
     1261 agtttcagcg ataaaaattt taaaagccat ttgcgagtat ttgtgccatt cgtggctaaa
     1321 catttttttg aaccatgaat tacacgaaaa gttcttggca aaaatggact atgactccat
     1381 aatagccaga gaatgtggat tgaaagagat atttctcaaa cgataaataa gagtaaagat
     1441 gtgattcaag tgatacgagg accaaggcaa attgggaaaa ctagtttgtt tttaaagttg
     1501 gatccagact ttaaagaatt atctctagac gatccaatcc taagagaact tgccaaaaac
     1561 gatcctgatt tatttttaaa acaattttcc gataaaaaga tttttattga tgaagcacag
     1621 tatgctcctt ctctttttcc ttccctcaaa agaaaagctg atttatacaa aagagagaac
     1681 aaaggtaggt tacatacaat tatccgctta tctggttcga atcagatttt aatggataaa
     1741 aatgtaaaag aaagtttatc tgggagagct gggttttttg atatgaatac attgagtgtt
     1801 tctgaaatct tagcatttaa aaaatacaaa atccaagata tattatttag aggtggctgg
     1861 cctgaaatct acgctgaaaa aaatattgat cccaaaaaat atttagatga ttatataaat
     1921 agttatgtag aaaaagatat agtattgagt gcaggaattc aaaagagtaa ccagttttta
     1981 aaatttataa aactgcttgc aggtcgtgtt ggaaatttat tagaatatgc atctatcagt
     2041 caggaagtgg gagtagatag caaaacgatt aaagattggg tttctgtttt ggaaagaatg
     2101 caaatcattt ctttactcat gccttattct acaaacctca ccaatagact ggttaagtct
     2161 cccaaaattt attttctaga tactgggctt gcgtgtcgtt tacaaggttg gacgtcagca
     2221 gagcctattc tcacttcgcc tcagcaggga ggtctatttg aaaatttagt tttctcagag
     2281 atttataaat tgaatttgaa ttaccaacta ggctggcaat tttttcactg gagaagtaga
     2341 gacggagaag aaattgattt tttaatccaa aaagatccca tccattttct atttattgaa
     2401 tcgaaggttt ctcctcaaaa ccttccacca ctgacagatt ttagagaagt aaaaaaagta
     2461 ttcaaagaaa aaatcccgaa aataattcta tgccaccaag aaggaaatta tattttaaat
     2521 gagaaagtcc ctatttctct gctaaaagag tttttgatga aataaaacca ttcgtttttt
     2581 ttccaccgat gaactgtgat gaaaaagtca agccaaacag actttttcgt catagagatc
     2641 accattttta acaattgtaa aagctatctt caataacttg tttgcgacag ccattaaagc
     2701 taccattgta tgttttccat tttttcttaa tctatcatac atttctctgc acacataatt
     2761 acgtttgata gcagacaatg agcacatata caaaagttgt cttgtataac tcattcccat
     2821 ttttgctatt gaacttttac ctttgacact agtgccggat tcagtaatac gcggacagag
     2881 cccgatatat gcagataatt gtttatagct tgtaaagttt tcaaagtttt ttgtgacaat
     2941 tattagttca atagcggata acgatcctat tccaggtata ctttttaaac acttatatgt
     3001 ctcactcgct ttctctttaa ctatttcact catattcttt tctaattcat caatttgctt
     3061 ctctaaattt ttaattgttt cttcaataaa tttgatagac gtcagatctg gacgaaccct
     3121 tgactttaaa gcatgcaatt gatttttaaa tcttgaaacg gtttctttaa gttgttttaa
     3181 aacagaacgt ctttgtgata aatcctccat gtattctgga ctaggttttt caggctccgg
     3241 agtcattttt aatccaaact ctgcaatcaa cactgcatct gaagaatctg tttttgttct
     3301 gcttaagttc atcttagaaa aatgttttat ccttagcgga ttaacgactg aaacctcaaa
     3361 accagattgc attattcttt tcactaatcg cgtcgaataa gaacctgtag cctctaatac
     3421 gctcctatcg tctttcttta atttctttat aaattttttc acaccttctt cgttatttac
     3481 aaactgaaca actttgtatt tagaattaga agatggaaat gcaacttcaa aagtatcctt
     3541 agccacatca atgcctatgt atctcatctt tattacctct attaaaattt tacttgtaaa
     3601 aaactaaatt tttttgagta attaaaatta cccaaaaaaa ggcttagcag gagagaaaaa
     3661 aattttactc tatcatcaca catacaagct cgttgcctac tgaactgtcc agagttgaaa
     3721 tttaggaaag ggaacctgca tcggtgacgg tcttctgacc atacacactt ctcggtttgt
     3781 ttctcctttc ctctctcttg ttaagttatt ttatttttct actttattta aaaaatctct
     3841 ttttttaaca tatgatgaac acggatatgc acggagtctc acggataaaa attttaaaac
     3901 ctcaccgcat actttcctgc aagtaattca ttattcaaaa acaatccatc ctttagaacc
     3961 ttctcttcgt ccaattcatt ctttagataa aacacatcca cgagatagcg atcatattta
     4021 tctttcccat gagttttgat aattagaaac ttgcaatctt tcaaacaaga ctcgataaat
     4081 cttttagagg caagtccctt tttggaagac gttaggggat tttgaggaga ggttgaaatc
     4141 tcgggagcgt ctaatccgcg tagccgcagg cgttgtttta tggagatcga aaagcctagg
     4201 tctattgtta ctagtaatgt gtctccgtct atgactcttt ctagataggc tttgtaggtg
     4261 aatcgttctt tgtggtttgt cttgagctta aaggaataat ccttgttatc cttaatggat
     4321 tcgacgattg agcctagttt gaattttgag agtttttctt tttcttctaa gagaatcgaa
     4381 aaacctaagt cgatatgcgg tatgctatct gcatcctctg ctttgtattt tctgactacc
     4441 ttgtaagtgt agagttttaa gtagggtcga gtaagagttt tcgcttcttc tttggttttg
     4501 ggttctggtt tggatttatt ttttttgtcg gctaattctt gtttgaccaa gcttaataaa
     4561 tcgcggttgg atagtttcct ttggattgct tttttttcta ggcttagtct tgtgttttgg
     4621 ttttctatga ggagtagact tacgtaatga ctccaagata attcggagtg gaatttcttt
     4681 gctttgtagt atagatagaa ttttcgcata tacccaagat ttctttcgga gaaacctttt
     4741 tggtattttc tgctaagatc tttcgatagt tgttttaaaa cttggtcgcc gtaattggct
     4801 cgtgcttttt tcttttgtgt gatttctatg attctttgtc ctatcttcca attggaggat
     4861 aatacagcct tgttccaatc ttcgtttcct tcttctagtg attgttcgta aattttggat
     4921 atgtctttta ggacgttgtg gtattgagtt tgggagattc cagaagattc ggtagccatt
     4981 tgtatgatgt cgtatattga aaactgtagg tagtcaagct gaaattggtt gtctggagtt
     5041 ttatggaaac tgagttgagt caataactca ccatacgcaa aagcattaaa tttgaggtta
     5101 ctggaatgaa aagcttaata ttaggaaatt ctaatgaatc aataaattca atagcctgcg
     5161 gcagcaaacc caccgttagg tgtaacccat ccctcagcgt tgggcacgct ggtgactcac
     5221 cttgcgtaag gtgagtcaat aaaatcaagg tagaatcagg tttttggaat ttgaagtagg
     5281 gtggggattg taaaatattt tctagatttc gcagacagtc tgcaaaatta tttaaagttt
     5341 agcggataat acagaatagc cgctattaga atcaaagtgt tttgacggtg aatcttttcc
     5401 agtcaggaaa aacaaaaacg cgatcccttt cgcaaaagga gttttaactt tgtgatcttc
     5461 ttgcccttct tgggaaaaag tctttttatt tctagcgtta gggatcgaat ggggtcgagt
     5521 tttttcatct gtttgaaaaa acttgaccag aatgagagcc cgacggcagt agtggaaaaa
     5581 agtcaatagc aaataagaac gagttgccgg aacgcccacc caatgaatca attgtttgga
     5641 agctagtagt taaaaacctt ccctatgatt tttaaattcc acttctctct ctggaagcgg
     5701 agaattttat tttaaaaaat ggtttagaat agaaagtaat ttgtttttat cgagaggctt
     5761 tgttaaaaaa ccatccattc cgcttgctag tgctttctct ttttcttcct tgaatgcttc
     5821 ggcggttaac gctaatattg gaactctctt tgcctttgtt ttttccattt ctcgtattct
     5881 tttcgttgca tcgtttccat cgagtccagg catttgtata tccatgagga tcaggtcaaa
     5941 atcatgtttc tccaccgcct caattgcctc tgttccattt tctgcctcat agatttttgc
     6001 atttggaaaa tacattttga gtaatgcttt tattaagagt agatttactt caacatcttc
     6061 tgcaatcaag attgaatact caacaaacga tagttctttg gattcgtgcc cgatattgga
     6121 tgattgattc ttaattggtt ttggtataaa gtctatctca agtgtgaatt gaaatgtaga
     6181 gccctttcca atttcgctcc tcacttcgat ttgtcctccc attttttcaa caagcaaatg
     6241 agaaattcta agcccgagtc ctgtgccacc atattttcga gtagtagagg agtctgcttg
     6301 tgtgaatgct ttaaatagtt ttgccatctg ttctggcttg attccaattc cagtatctat
     6361 tacactgaat gtatacgctc ctttcttttc tcctaaatct tcaaaaacga ttttaaattc
     6421 gactttccct ttctcagtga atttaaccgc attgtttaat aaattgataa atatttgctt
     6481 taagcgaatt gcatctgttt ttacgacagt aggtatatcg aaaggaatat ctacaattaa
     6541 ttctaaattt ttctcgtttg cttgcagttt aattatattc actgcttctt ccataagttc
     6601 aacaagattg gattcagtat tatctagttc taattttcca gactcaattt ttgacaaatc
     6661 taagatgtca gaaataacac ctagaagcga tttcccagaa gaaataatat tggaaataaa
     6721 attattctgt gtttcatcga gagttgtttg tgcaagtagt tcggaaaagc caataactcc
     6781 atttagagga gttctaattt catgactcat attggctagg aattctgatt ttgctcgatt
     6841 cgccgtgtct gctaatcgtt tcgattcgat tagctctctt tccacttttt tcatatccgt
     6901 gatatcaatt gtaacgccta acacttttcc gccttcaatt aagatggcag agagtagaaa
     6961 aaatttagtt tctccttgtt ttgtgattag ttctaattcc gcttccagtt ttccttcaga
     7021 aattagtttt tgaaagcgaa atatttcttg ttctcttttt tcagcggata ctaagttagt
     7081 aatattcatt gagagaaatt ctgtctgtgt gtaacccgtg ctttctgatg cagctggatt
     7141 gacttctaaa taatttcctt gtagatctgt gataaagatt gcgtacggag ccatttgaat
     7201 gtagctcttc catttcgctt cactcatttt tattgcatct tcagctaatt tcctttctgt
     7261 aatatcacgg ctgattccta agattccaat taattctcct tccggtccta ggtaaggagt
     7321 ctttaatgta tcgagtagta tcttttttcc gtctggataa gtaacccact cttcatttcg
     7381 tctagcgttt aattgctgca tcattttgtt atcatgttct ctaaaaaaat cagccaattc
     7441 tttagaaaac agatcgtagt cagtcttgcc tatgatctct tctctttttt tgcctacgaa
     7501 cttagcaaat tctggattgc atcccaaata aattccatct atgtctttga aaaaaattaa
     7561 atctgggatc gaatctaaaa gggaagtgat tagagccgtg tttttatgca gttcttccat
     7621 tgtctttttt ttctcggaaa tgtcttctac gatcgaccaa atataagagt ttcctgccgc
     7681 atccttgatc aagattccat tcaaattgat tggaattaaa cttccgtctt tttgaatgta
     7741 ttctttttcg taaggaccat atctaccggt tttagttaat gactctaatt gtttttcttc
     7801 ttctgattca tatttttttg gagtcagttg ccaataatcc aattgattga gttctgattc
     7861 tgtgtatccg catatatccc gaaaggcaga attgaattca atgtattttc cattcatatc
     7921 cgtaagtgca atacctaagg gagaaagttc ataaagactt tggagtttgt cttcatttgt
     7981 tctaaaattt ttttctagtt ttttgatttg ttggttttct tggtttaact tttctacttt
     8041 taattctaat tctttttgaa tcgttatatc ctgagtcaat atgatcagct gatttttagt
     8101 tgagacttga acgcaaacgt cataccactt atttaagttc ttttggtaaa tctgtgtttg
     8161 ttgattttta ttttcagaaa gaaaaattag gaattgtttc cagttctcat ttgcattaac
     8221 gatagttgat tcaatctctc tgatcttctt acctaggatt tctttaatat ctaactcaaa
     8281 tacccttaga aaaaaaatgt tagcttctag gaatgtaaat tctgatctac tctgattcaa
     8341 ttcattttgg agaagagcat agccaagagg ataatcttgc agtaaatcag atgcaaaaat
     8401 cataatacaa ttataaatga gaatcaatat tttgaaaata aaaaatattt ctaaaatgga
     8461 aaacttattc tttcaagatg ctaactgtta ggttttgttt tatttggatg ctacttgtag
     8521 tgtataaaaa tttgatagat gaatgactca ttttttataa gtaaaaaaat tctaagatag
     8581 tcatactcca aaagagaaat gttacaaatt tatttctctc tcacgaggaa gtaaagtgca
     8641 tccttctgta aataaggaat ctcacgaatc agatcttaga tttagaatga gcaaagtttc
     8701 aattttatgt gtttctactt ctaaatagtt agtcgatata cgttgtgcgg aattgtattg
     8761 aaaaagattg tctgatgtgt atttgaatgg gttttatatt ttacaataaa ctttagactt
     8821 accattcatc ctttttttat agactagatt tctaaaaact tttacaactt tagtagggtg
     8881 caatttcatt caatataaaa tgtcttcttc ttgatttttc attttcaaaa acagggtagt
     8941 ttcattagtg tggaactatg cccaagagat tagtcccaaa aagtcttaca tctgaaaaaa
     9001 gggaaaatca tttaactact tacctgaggg attttcagaa aagaggagat tttaagaaat
     9061 atgtttttgt atctcttatt cttttagttt tatttgaaat cttaattggg attggcttta
     9121 atcttatttt ctcagaaact ctagaggaga atcgcaaaga aaattatact agagcatgga
     9181 ataattatct aaatcaaaaa tggaagtcag atcatagtaa aatcactgga tatgtatggt
     9241 ggagtgatat ttggaaaagc atgtataaag gcgatttaaa acaattagat actgcctttc
     9301 aagctgatgc ttctttcaaa gaaaactatg atttgttttt agtttttacc aatccaaatt
     9361 cagatcctgc ttttaagata gtcggtaaaa atcttaaaag ttttgattct gtagataata
     9421 atttaattca agcattcttt cgggagaaaa atctaaagcc tggcaatatt cacacaattg
     9481 aaaaattaaa agacaatcga tactttctca tttctgtttc tgcgttatgc gacgacttag
     9541 gaaaccctat ttttccaggt attgccttct ttgcttccga cttagagaaa tttctaaggc
     9601 ttgctgagga ggtaattcct gttaagatgg aggttaagtc tggtttacct ccgaaagata
     9661 tttatcattt ttttaatatg ggcaatacat taacaaattc aggaaatttt tatatcgaag
     9721 tgaagcctga atttgaaatt aaaactttag tcataaaaac actcatcttt tttatttcca
     9781 ttcaacttat cctgactatt tctttgtttg tattaattgt tcctcggtat acccgaagaa
     9841 aaacagaaaa gttaaaagaa attatcaatg cttctgaggc tttaaattat gaactgatga
     9901 ataaagtaga agaattaaaa attgccaaag aagaaattga aaaatcggaa gcgaaataca
     9961 gtcatttaat agaaagttcg aaagatatta ttttttcttt tgatcataat ggagtaattt
    10021 taacagcaaa taaagcgtta gtagaatttc taggatttaa aaaagaagat ttattaggaa
    10081 aatatttctt agatcttgct tatagaccag ataaaaaact cgattcactt gaaaaacaat
    10141 tgttaatgga aaagttcgaa gaactaaaag aaaacaaatc ttccgtatct ttcgatatga
    10201 cattcggaac aaaaaataat gagcctttgc aattaggcgt aaaatgggaa tatgttaaaa
    10261 ttggtgaaaa ttttgtcgtc tttggaaaag catttactgt ctcagaagat tcaatgctca
    10321 agtatgtaga atctgagaat agacagtatg tttttaataa ttacataacg cttgcagaac
    10381 aagtatctca gcgaatcaca gcaaatctaa taaagcatct agattcaaat gatgttttta
    10441 atattaggat ttgtgttcga gagattatta tcaattcaat cgaacatgga aatctaaata
    10501 ttgattataa tacaaagact gagcttagaa aagtgaaagg aagatacttt caatttttac
    10561 aagaaagaca aaatgatcct ctttataaag atcgaaaagt tactgtcttc tattccctaa
    10621 aatccaatcg agtctctttc ttggtaaaag acgaaggaaa gggttttgac cataaaaaga
    10681 tgcttgcgga taacgctgat accgctaatg caaatttttt agatcatggt aggggaattt
    10741 atatgactaa aaacaccttt gataaaatca gatacaatca agctggaaac aaagttttat
    10801 tagtaaagta ttttaaaaaa gattaactct tcttttttat tagaatagaa aattctctgg
    10861 atgaattcac agaaaaaatt tattcgattt ttcgaatctt atgattattt gtatccgcga
    10921 catataaaac atttccaata aatgtcattg ctcttggaag attaaaagat gcatttgttc
    10981 cagttccgtc acttgatcca gaacttccag agcctacaaa taccgaacca atgcctgtat
    11041 cgatatcata tttataaatc ctatgccctg tattttcact aacgtataaa tatttaccat
    11101 aaacaacagt ggcatgagga tccgtcatgt ttgtaaaggt tgagacactg cctgatagac
    11161 tagtatcaat ttttcgaatg ttattatttc ctttatctgt cagaaaaata taactttgat
    11221 agaaagtaat gcctacaata tcttggctaa aactagccga aagaagaaga ccattgtttg
    11281 ttccaggaca agtagaaccc gcgtgtgttg tgacaagagc agatgtggtt atctttctaa
    11341 ttctacaatt atcttcatct gataagtaaa aatcagaccc gttgtatatt atgtaattag
    11401 ctgcattgaa tctagctgta gaacctgttc catccgtgta tcctagcgtt gcagtagcag
    11461 gtccggcaaa tgtggttacc gtgagatttg ataaatttaa taaacgaatt gaataattat
    11521 ttctatctac aataaataga tttgttccat ccgttgtaat gccagtagga ctattgaatt
    11581 gagctgataa gcctatcgca tttgtgtatc ctaatgtgga gcctgcaatt gtgctaactg
    11641 tttggttgtt catatctagt ttacgaattc tattattgct cgtatctaca acatagagac
    11701 tattgcctaa cgctacaata ccctgtggtt gattgaatgt gctcgttgaa aaagaaccat
    11761 cagcactccc taagcctcct gttccagtaa gagttgttac gattcccttt ggacctgttg
    11821 agcaagtaac tgttcctgaa atatttggat ccatttgaat acaaaaggga gtatacagaa
    11881 gtttattccc attgctatct tttgcaataa tatcgactcg gtaaaattga tatggctcta
    11941 acccagagat tgtataagca gaaatatttg ttgtatagtc tagttttaaa actgcatttg
    12001 cctctacctg atcgatagtc gtccactcgt ctggatagga tgcataaatt ctataattaa
    12061 ttgttccgtt cattccgctt ggtggcgtta agttaattgt aacttgtgta taggtccttg
    12121 cacctaatga aatattacct gattgtggag aggaagtcgt tgtgtttgaa actgtcggcc
    12181 tattacttag ggaagctaca atagatgtag tggaggaatt tctcatatca aatggactct
    12241 tttttgcttt taaacaattc aaaaagagta agaaataaat ggagtataaa attgagatta
    12301 gaaagaaata gaataacttt ataatgtgaa cttgcatgct tctctattaa ctcctttttt
    12361 taaaactgcc accacttatc ttggaactaa atatttagat cctttgaaat cattttcatt
    12421 cctatttcta gaaatagatt tcaatgtttt cgataaaaac taaggtatgg atttaagatc
    12481 ttcatcacga ggagtctctt aattaatact cacttgtttt caaatcaaat tcaaaaggag
    12541 tctttacgaa aaaggttttt cttctaaaac tttaatagtt taatctaacc aagtttcctc
    12601 tagtgaatcg ttttgatcga tgcaagaaat tctgtcctgt ggcgcatttc tgccattcta
    12661 ttcacacaat tggtagaatg caagggatat taaaagcgaa tagttaagat atttctaggg
    12721 gctaattgag tttagcccct tagaactagg aatgtcgaaa cttactttcg attgattctc
    12781 tcaacctgtc tacatacccg aagagtgctg gcacaacaat caaggttaga agcgttgaaa
    12841 gaataaggcc tccgataatt gcaattccca ttgctgttct agatttggct gcttctccta
    12901 gtcctagtgc aatcggtaaa gttcctgcga tcatcgcaag agaagtcatt agaataggtc
    12961 ttaagcgaag aacacctgct gttttgatcg cttcctttct atcaactcct gaatgtatgg
    13021 cgcgcatcgc ataatctaca attaaaattg agttctttgt tacaagtccc ataagaagaa
    13081 tgatcccgat catagaaaat agatttaaca tttctccaaa gagtgcaagt gctaaaaaag
    13141 ctcccgagaa agcaggcgga attgcaatca aaactgaaat aggtgttata aaagattcat
    13201 aaagagaagc aagcactaga tagataaaaa gaagtgcaaa tccaaatgca ataataatgt
    13261 tttgcatgag ttctctaaaa tcttcagact gtccataaaa cgcataacgt attcccggag
    13321 gaggggggaa tgtctccgct aatattttct tcgccatatc ggttgcattt ccaatggctc
    13381 ctgtggaagt gagattcgct cgaataggta tgacacgaga ccgatcctgt ctactgatct
    13441 tagctggacc aagtttctct tttccactgc ttacaaaatt aagaggtatc attttgaaat
    13501 tgacgttagg aacttttgtg tctaagtagg cggaacgcaa atttcgttgc tctggcttta
    13561 aacgcatcag tatatcgtat tcgagtccct tatcatgcag tttcccaaca acgccaccct
    13621 cgatatgata cctaagttct gcacctgctg tgataggagc aacacctaca ctttgcattt
    13681 ttttaaaatc aaattcgatt tgaaattcag gctttccagc ttggtaatct ctttcaatat
    13741 ctgttagatc cggtattact tttagttttt cgattaattc ttttgagtat ttttctattg
    13801 cctctaggtc atcacccatg atatttaagt taaatggata attgactcct ccacccacag
    13861 cgctataatc attcaccgag ggtctagcat aattaaactc tttcatcatt tctcgaactt
    13921 cagattttac aactgctgtg cttcttcgtc tttgatgcga tggaactaac gctattccga
    13981 tattcccaat ggtaggaaat ccgtctgggc ttcctacaac agttgccagt ttgtttaatt
    14041 ctggaatagt ttttaatttt tcttcgatct tttgaattac atcataagtt ccttgtaaag
    14101 aagttccagg tggcatatct atagaaacta aaaattcacc ctgatcgtta ggaggaagaa
    14161 aggtcttttt tacaaacttt aatgagtaaa tactaaaaac aaaaacaaat gtagctgcga
    14221 ttaatacaat gattggtctt tttatgacaa agtctagaat gattccatat actttctcta
    14281 accatgtttg gaaattatca aagattttta ctaagaagtt tggattccca tgtgcctttc
    14341 ctgcaaaata agcgctaagc ataggagcaa ttgtgagagc atcaaacaaa cttacaatca
    14401 ttgcgaacac aactgttaaa ccaaattgtt tgaaaaactg acccacaatt ccacttaaaa
    14461 atccaatcgg taaaaacaca gcaatcaccg ttagacttgt agcaacgact gcaagagtta
    14521 cttcggttgt cccaatctca gcggcaataa caggaggatg tccttcttct aattttctaa
    14581 aaatattctc tctaacaaca atggcatcat cgaccaagag ccctaccgtc agagataagg
    14641 cgagtagggt cattacattg attgtaaatc ccatcgcata cataatgata aatgctccga
    14701 gaagactatt ggggagagca agacctgtaa taatcgtgga tctaacgttt ccaagaaaga
    14761 aatatacaac aatgactgtt agtaagatcc ctaatataag cgagacagat acatcttcca
    14821 cattggctcg tatccaaatc gagctatctc ttacaacaat aatatgtgga tttccttttc
    14881 tatcttttaa aacagtttcg tttaactttt ctactttttg taagactgaa tcaacaacgg
    14941 caactgtatt tgccccagat tgtttatata catcaataaa aagagaaggt tttgttagtc
    15001 tttcgatttg tggaggatca ccttttcgga tactattgat aaacccttgg atgagactgt
    15061 gtgttggcgg atcttctttg gtaggtgcat acaaaaaaca catggttttt aagtcttcaa
    15121 tcgtatctac cactcttgca attttggaca ccgggacaga acttccaagt tctcctccga
    15181 agaaaacctg cgcttcttct atttcagata atttttcgaa tcgaccaatt gttctgtagg
    15241 atacttcaga tttgccgctt tcaaattttc caaccggaac attgagtcca aagcttttta
    15301 attggttccc cataaaaaca tttgatagag aatattcatt tagttttttt ctatccacct
    15361 cgatttggat ttgtctcttt gctcctccaa tgattcgaac ggatccaaca ttgttgactt
    15421 gttcaattcc tggctttaat tcttctttcg cgatatcata tagttctact ggaggaattt
    15481 cgccaaagag agaaagccta acgattggct gatcggctgg atcgaatctt tgtataactg
    15541 gctcatcaat atcgcttgga agttctcttc taattctagc tattttattt ctgacttgtt
    15601 gttctgaatc ttttatatct gctgttagaa aaaattctgc tattacaatt gatatccctt
    15661 cttggtttcg agacgatata cgcttgagtc ctgatatcga tccgatttct tcttcgattg
    15721 gtctagaaat taaagtttca acttcttctg gtcctgctcc tcggtaaaca gtcgaaactg
    15781 agatgactgg tatatttaca tcagggaata aatctactcc gagtcgattg agagcaaacc
    15841 aaccagtaaa aataattaaa attacaatac ttgagataaa tatgggtcta tttatagata
    15901 gctttgctat gttcatggat acattgtttt gaatcccatt tcacatacaa taaaaataat
    15961 tagaaagaga tctattcttt gtgtttcaac gatgtaattc taagaactaa aaaataggta
    16021 aggaggatca aaatccgtaa gaaacaaata aaacaaatat tgtttactta tttcttacca
    16081 ttttgttggt gtttattaaa aataatatct agctccaatt gctataccgg gaccgaatcc
    16141 acttctatca ctgagataaa tattgatcgc caattctccg taaatttcaa agtttttttc
    16201 gcgattcata taagtaattc ctatcggaat acgaagtcct ggggagaaaa aataatgatc
    16261 tctctccaca aaacgccctc tccctggatt taacttttca tatctttcag aaaaagagat
    16321 tcctccacct gcgtaaacat acaaagaagg cttaatttct gcgaaattat ataaaaaaac
    16381 aaaatgaaga tattgccccc atttttcttg atagccaatt cccagtgttc cttctaacgt
    16441 ttttttatca tctaaccaat atttacctgt aataccagtt ggagaaaaaa gagaaatacc
    16501 aacaccaagt ttttttccag tatcggaact ggtagattcc tttgataaaa tacctagact
    16561 cataaataac aataggagaa ttattatttg tttcattagt atgtaactac ctttagtttt
    16621 tctttgaact ttaaatttag tttgtcctcc aattttctag gaaatgtgat tgtgtttgga
    16681 tcaccatcac ttacatacga atcgggtgat tctgaatatt tttctatcat tttttcgata
    16741 ttatctttga aagcttgcac ccttcttaca tggattgaat caaattcttt atcataaggc
    16801 gaatagctag gacggttgga ataatcaaaa acaagaatat gtttatactc aattcctttg
    16861 tagcttcctg aaacaagttt tgattcgtaa tcctttggag gataaattcc aaatggcatt
    16921 gcgagagatg tcattttata atcaggtaga tatttattta aaagtttttg actctctaca
    16981 atttgttctc caatcttatc tttgtatttt tttaggtttg catgccaaag agtatggttt
    17041 tgaatttcgt agttattctt tgttaagaat tctaattttt tagatgtaag cttttcttga
    17101 ccaaacaagt tgtttggatg ctttgcgcct ggtaaaataa aaaaagtagc cgttagtgga
    17161 aagtctggat tcttcttttt gaagtcctcc ataataccaa cagcacaatt gggagaaatg
    17221 ctaccttttt catccatatc aaactggctt tgtgaggaat catctagagt gataaataca
    17281 ggtctttttc cctttggaat atttagattt cttttttcta aatcagaaat tttcaaagga
    17341 tagtaattcc ctttttttaa aagagccaga tcatctttaa atccttgaat tgttcttgtg
    17401 taagcagaat ctttttcacc gatcacatgg taagtgatga ttagaaattt tccgagttca
    17461 tttggggctg attctgcgaa tgcaggaata taaaataata atgtaagtaa gtaaattttt
    17521 aggaatctat aaaacatgaa tccatctttt tcaaaaaggc attctcgaaa atagaaaaat
    17581 tgttttctta tcttcttaaa ggatttttta ccacgaagcg cagtaggaac aatgctgtta
    17641 gcataagaat taccaccgag ggcaccgata actgaacgat acagaaacga ttgataattt
    17701 tggattatcg tatcttttat cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17821 nattttattg tttagactaa atcgaagagt ttttactcct cgtctttgtc cttttctttc
    17881 cccagcatca cttatatcaa ctgtaatcgg aaaattacta gatgaattaa aagagtctcc
    17941 atcattaatg taagtcatgt ttttaccaat cgtgagtatt aaacttccaa tcgaaggagg
    18001 tcttgtatct tttactttgg gtagaattct tagaggatcg attatttttc tgcctttgtc
    18061 tgtcgcaact acaaagtgaa ggtgagaacc actcgaatga cctgtatttc ctgttttacc
    18121 tacactatct cctttttcta aaactttttt ttctaagacc tctaaagatc ttccatcttt
    18181 taagtggtag taggcagata aaatatcatt gtcatgcaaa acccaaacac tatttccgct
    18241 cccactctca ttttcaaaag gattgtccga attataatgg ctatagatca cagtgccttt
    18301 ttcgatagaa gatacctttt cattatcaga ggaaacatcc accccattgt gaaaatgatc
    18361 acccctagat tctccaaaaa gagaggaaag ttttacttcc aaattttccc cgccgacggg
    18421 ccagataaat ccagcgggta gagtttcttt ttgtttatct tgactcaaca agaaacagag
    18481 tggtaggagt agaaatagaa tagagatttt tttcataaaa atgattccgt attttttttt
    18541 aaatgactag atagattcaa aatttattat accaattctg actatgaaat tatcacagga
    18601 agaagttttt agtttagtcc aatcctgcgc aagtggaaat gaggcctctc ttaaaaaatt
    18661 ttttgaagag tttgccgagg atatttataa tttcccaatt cgcgtgtttc atttggatga
    18721 ggatgatgcg agtgaatttt ttatctatgc ctatgaaaga cttaaatcag gaaagagatt
    18781 tcatactttt gtaggaaagt ccggttttaa aacatggctt tatacagttc tacgcaatat
    18841 gctaatcgac tggcgtagga ataaacgtga attaaaaata gtttccaatg taaaagtaaa
    18901 ttcggatgga gtagaatatt actcgattga aaatgagcca gatttaaaat ccaatttaca
    18961 gaaagaagca gattctgttt catctcgttt tcaggacata ctctctgaga ttaaagtgga
    19021 gagtcgaatt attttcaaat tagcatatat ctattactta aatttaaacg atgaagagat
    19081 tgatttttta gtcactcgaa ctggcaaaac aaaagaacaa atacaaaaag acatcttgaa
    19141 tatccgcgaa aatctttccg aaaaagagat acaaaatctc caaagtgaag ataaaattgt
    19201 ttctatctat atgaatatct tagagctaaa ggaacaacaa gagaaagaaa agcctattgt
    19261 ttttgaggat aatctaccaa attatgataa ggttcaaatc gctatcaata agaaatatga
    19321 acaaagaaga aggctgattg taaagaaatc gaaaggtaat tttattacca gaacgccgta
    19381 tcgggttatt gcagaattgc taaaaatacc tgagggtgga gttagtattt ccttacagag
    19441 agtattggaa aaaattcaaa aaaaatttcc taaacatgaa atataaagcg tctttattca
    19501 gtcataattt acgagaggtt agctatgaat tctaaaaaca atcaaaatga tcagcctaac
    19561 tcaaagaaat accttgatct atttttaaat gagggtcaat tacatgaaag cgcattggaa
    19621 aatgatacag actttcagga attgattcaa ttagaagagt ccattgtaaa tacgattcag
    19681 ttagaaaatg agaaacactt agaccacata ctagagaagg attcttcctt ttatgaggat
    19741 ttagatatag ctaaggcaaa tctaaatatt accccagaca gaaacagacc actcccactc
    19801 tatttaaaaa actatatgga agctgatttt gcagcacaga aaaaagaaga ggatcgtttg
    19861 gtgattcgct ttggtaaagc aggagtaaaa cttcttggtt ctttcttcgg acaaacagaa
    19921 attagtcctc tcgaactttc tattccttct gttcgaaatt ctataaatac aactcctcaa
    19981 agtattagta tgctcgaaaa aacagaccaa gggaatttgg tttatcaagt gatccaagaa
    20041 aatgaaaacg aagcctattt aagtgtgaag tttgatcctt cttttgttgc tatttataac
    20101 caagtaaatc tcagaaaaga caaccgtttt atctattcta gtaatattaa ttcggaagga
    20161 attgtatctt tttctggatt gaaagaaggt gtttataata tcgagttcac aggaaagaac
    20221 gtttcaaaaa ggtttgactt atctttattt gcagataagt tgtagtcgat gatcgactta
    20281 ctgattgata gagttggcaa tgtaaatatt tttaattttt taaatgccgg acaatttcat
    20341 tccgaatctc acattcagtc ttccattgat accgatttaa tttctgagta tgtaaaggaa
    20401 gttgagaata tatctaaact ttccaataat tttatagcca atcacgaatc caactttaga
    20461 gttaagccta acgttttaaa agatttaaaa gttttgggtg agacttttta tgagcaattt
    20521 tttccttcct ccatttctga taatcttcga tctacttcag agaaatatct tcattttaat
    20581 atagatagta gccttgccga aattccttgg gagttattgt atgatggcga atgttttttg
    20641 tcagatcgat ttttaattgg cagaaccatc aaaggctcaa agacatttcc acaggaagaa
    20701 aacaacgaaa agttgaaaat gctaatcatt gcagatccaa cagaagattt agaatgggct
    20761 caaaaagagg gtgaggtttt atttcaggtc ttaaaagaaa aagttcctgc ttctgtattg
    20821 gatctagaat ttatcgcagg ctctcaaatt acaaagctaa aacttctttc catgatccga
    20881 ggaaaaaata tcattcatta tgcagggcat ttattttttt caaatgatcc aatggaaaat
    20941 ggatggcttc tcagtggaga taaaatttta aaagcacgtg agattaaaaa tagcgctttc
    21001 tcaacaaatt tagttttcag caactcctgc aattcttctc gaagtgttgg aattaaaaac
    21061 tctaagagca ttattaatga ttttgcaagt tcctttttaa tgtcaggtat aatgagtttt
    21121 attggaacaa agtgggaagt tgcagacaat tcgactactc ttgattttac aattcgtttt
    21181 tacctagcgc tattcaatga taaaagtttg ggtgaatctt tacatattgc aagggagtat
    21241 gctcgaaaaa attctgatcc attggattta atttggggaa attattctct ttacggtcat
    21301 ccagatcgaa tcatcctaaa caaaaacaac aatcgtagga ataaaatcat agaacctatg
    21361 atgattcgaa aaacatatcc tacaccgatt gcaatatctt attctaattt tatttctatt
    21421 gagagaatgg attctactga aaataaaatg aaagcatttg taaatagttt cgaggaattt
    21481 acaaggttaa ttacaatcat agtgattagt gatcacaagt ataaagctct caaccaaaat
    21541 tttgaaaata aggaagatct aagtttagag tatttgtggg acattatttt taaatccatg
    21601 ttagatttta aaaaattaca aatatcaatg gttgttgaat ctttccttgt tgttttattt
    21661 tcgaataagg atttgattta taaaattatt tcttggatcc aaaaatttag agcaggtggg
    21721 cttgacacag aaagtttaga aggctatcta ataacgtctc aatactatta tgagaattta
    21781 cttgtagagt tgaatgagtt tgaaaaatgc tatatcttct atatttcgaa tgattctgat
    21841 cgtcactgtt tattttatgg atacaacaaa aaagaaacag aaatagatgc tacccaattt
    21901 ggattttttc taaatcgatt ggaggaattc agaggtagag ttattttaat tcataaaaca
    21961 aaaaaatcta tattaccctt aattggaatt cagatcaaat ataaaaatga tgaaagagat
    22021 aaggatttat ctttagactt catgaatttt agagataata ttgttcccag attgcaatga
    22081 gtctatgtca atcctctgag tcttcatgtg gcgcttgctg tggaattttt aatttagatt
    22141 acccacgaga gcagattaaa aaaattttga aagaacgcac tgagtatttt gaggatactg
    22201 tagattattc tcgaagagag accgttatat cctttagaga agtgtttgaa aaaaaagaat
    22261 ccacatatcc caaaaaagat catacaattt ataactgtcc cttcctaggt tatatcaata
    22321 aagattttac tagaatcggt tgtatgattc atccaattcg aactggagat cccaaaagtc
    22381 aaaatttttc tttttacgga tcctctattt gtcaaaccta tgactgtaga aacaaagatc
    22441 gaaaaaatgg aatggattgg gaagattact tcaactcact gaaattagat ttctacgatt
    22501 actctcattt ggcaagtgat catattctga ttacaagatt ggaaagcttt ttcgattctc
    22561 ttcaaattcc actgaaagat atttttctaa aacactctcc acttttaaaa agaatttttc
    22621 aatataaagt aaatcagatg aatcatctta cctcatttga aattgatatg aattcaaata
    22681 ccggaacgcc atttgaaaga ttgattgaga tactgagcct tgagccagat catcctttat
    22741 atctagaatt aaaattgttt tgtaaggagt aatgttttgg ggggagtggg ttgccactgc
    22801 cggaatcttt tcaggcagtg gtataaaaaa agattaagct tttttttcag gaactaaatt
    22861 tttaacctga gttttagctt cttcgaattt tgctttggca tcatttaata ctttttctac
    22921 ttctctgaaa gcttcttctg tatattttct gacgttcata gcagcttcgc tgttatctgc
    22981 agcaccttta tcagctaatt ttttgaattc tgtttctaat ccagcaacta gtttctcagt
    23041 ttctgattta acagtagtag cagcaccaat cacaaaattt aaagcatctt ttatttcttt
    23101 ctccatggaa attacctcat tccgaaattt tattccattc agtttatatt ctttcttttc
    23161 gtcaagctaa aaagcggaca tagcgcactc actatttctt caaaagcatg gttttagaga
    23221 tactagaaag acttcctgca cccgcgatat tcattgtatt ttttaaatcc gtagtatatt
    23281 ggtggattaa atacttcact cccgcaaaat caccgccaac cgccgagatt gcaattggtc
    23341 ttcctacaag aactgcctga gctccaagtg ctatcatttt aaaaatgtcc ataccacttc
    23401 gaattcctcc atctacaaga acaggaattc taccttgcac tgcttcagca attttttcta
    23461 ataccctagc ggttccaggc atttcatcta atactctacc accgtgattt gatactacga
    23521 ttgcatctga gccagcatct attgcaagaa ttgcatcttc tactgtcatg attcccttta
    23581 gaataaacgg aagttttgtc atacttctaa ttcttgttaa ttcttttaga cctctgctcg
    23641 gagattgttg gtttttcaaa atcatagttt taaaattgaa tgcatcaatg tccataccta
    23701 gagcaaatat attttgagct tctgcttctt tgaatctctc ttctaaaaga ctttcatcgg
    23761 ctctcggttt acaaatcaga attccattgc catttacttg tcggattgca tctaacattt
    23821 ttttatattt gtcaggagta gctccatcac caagccatgc gatagttcca gactcattac
    23881 atccttttag gacagcaacc gaatattggt attcatccat cgcattgttc atattggtag
    23941 ttgcacccgt cataggagca gccattaagg gggactgaaa agatcttcct agaaaattcc
    24001 attcaattct aggagaaaca ctttcacgaa tatatctagg tagaatttta tattcagaga
    24061 gagcaatgga attgtctttg aatgtttcca ttcttccaac tccacccatc cctggaactc
    24121 ctgaggcaca atcagttcca tcacaaacct tgcacaccca gcagatttct tttccaaatt
    24181 tattcctagc agtttgattt atactttttt cagttagtga gtaatagcta tctgattcaa
    24241 agtgaatgat ctgttttgct tcttcgaaaa ttttttctgc tttttctaat gtctcggcgg
    24301 tgatgatgat atgtcctgat ttttctatat tattcgttgg gtctgtgata atatctccca
    24361 cttttgattg tagaaaaatt tcattgactc cttcgatttc ttttgcttct tcaattcctg
    24421 atattgatat aattttacca ggagcagcga gtaatgatct ctcaatggaa actaaattta
    24481 attttggttc taagtcgtca ggttcttccc caaaggctat taaaatggca gaacgataaa
    24541 gattaattcc tgtcgaaaga gggtaggtgt aggctgacat aaaaccacca gataaccttg
    24601 cagcaatttc tccaatttta acaccatcag gcgttatctt tatatcacct tttcctgcac
    24661 ctaacgttat accaagtgct ctcattccct ttttcattac atcttctact tcatctaaaa
    24721 cttcttttga aaattttgat ggcatattgt gtccgatttc aatgaaataa ggttctcttt
    24781 cgataattcg atctgcaata ccagtgattt ttatattccc gttccaagtc agtgcgtcaa
    24841 ccgacacttc aggtcctacc atgaattctt ctagaatcac ttctcctgta ggtgaatatt
    24901 tttttgcatg tttaaaagcg gaatgtaatt catctcgatt attaaccttt attactcccc
    24961 gtgctcccat attatcagaa ggtttaatca caagtggaaa tttcaaataa tcaagtgcat
    25021 ctctcgcatc ctgaatggtc cagataggag caaaatttgg aattggaacg ttaaattcct
    25081 ttaagcgttt tctcattttt actttgttgg atgccgcttc cgcatcaaca tatcgaatcc
    25141 ccggcagtcc cagtgcgtta gctaccgcag caacagtcat acttgcatcg gttcctgcag
    25201 tgatgacagc atggactggt tccttttcgg aaaatctctt tgattctcga accattcctt
    25261 ctatatcttt tgtgctcatg ataatttttt cgtctgcgag tttgaatcct agagattctg
    25321 gattcatatc tgcaactaca actcttagtt tcatagattt tgcagtttcg attaagggaa
    25381 cttgcaacaa tccaccacca atgattaata tggttttgcc tttaattttg ctcattttaa
    25441 ttctttatac taaatttaaa tcgttgacta tacttgtttt gattttcttt aatttgatac
    25501 tgcctttctc aatgattcca cccatgagca gataatcgcc atttgtcgga taaaaaacag
    25561 aggattgtcc tggagtcacg ctttgcacta actctaaggg tttgacaata atcttatttt
    25621 tttccttttt gacttcgcat tgaactggtc tatgtctata acgtatttgc actttacacg
    25681 ttaattcttc tccctcattt aaaggagtta atccttgata atttacatcc gttacaatga
    25741 attcttcgca gtaagtctct tcttcctctc caagaatgac tgttccatct tcttcgatag
    25801 aaagaacata aagaggattt ttccatgaaa cacctaagcc ttttctttgt cctacagtga
    25861 aaccttcttt accagaatgc tttccaacta tattcccatt cgaaagttta aaaaaaccag
    25921 gagtaaaatc cacattcttt ttctttaaaa agcttctata gtcgttttct ggaataaaac
    25981 agatttcttg tgattcagct ttttcagcaa tcgatagacc aaatcgtctt gcgtgctctc
    26041 ttacttccgg ttttgtcata ttccctaaag gaaataccgt attttttaaa ttctcttggg
    26101 ataaaccata aaggtaataa gcttgattct tttttgtatc gactccattt gcaattgcat
    26161 aacgaccatt ctcagccaca tgaatatttg cataatgacc agtcgcaatt ttatcgatcc
    26221 ccaatacttt tcctttttcg aataaggacc caaatttaac aaaggtattg cactcaacgc
    26281 aaggattggg agtcattcca ttttgataat cacttataaa acgatcaatg accttgtccc
    26341 caaagatttt ctccatttta ataacataga atggaatatt aaaactaaga ccaacatctc
    26401 tcgcatctct aatatcttca ggagaacaac aagatttttt agtagtgtca caatttggag
    26461 cttcatattc ccatgtctta atatttactc caatgacatc atagcctttt tctaataata
    26521 gccctgctgt tacagcgctg tctactccgc cactcattgc aactatgatt ttttcttttt
    26581 ggctcattat ctcttcttaa tatataattc taaattttga tagtctatca gaatggaata
    26641 attttcaacc caattcattc caagaattcc atctagattt ccttccttgg atagaaaatc
    26701 ttgtatcgaa tttcctgata ataattctat ttttttttgg cataggtttt cagctacaca
    26761 aagattttct ccaatatgat aaaacgtgtt ctgttcttct cctcttaagt caatgaaact
    26821 agcttttccg cctcttttta aatttagttc agctgctttc gattggttta aataggaaat
    26881 tcctgcacct gtatctaata aaagtttaca aagacgattt tcgattagtg catgaactag
    26941 aatatgtcca tccgcaaaat ggaatggtat tttagtaaaa ccttcgaatt tctttgtctt
    27001 tatatttggt tgtataatat agacattctc aggcaattct aaaaataaag aaaattttga
    27061 taaaacatca tttcctatca atccatcaaa tgcaaatctt gaatcaaatt cgaaagtatg
    27121 aaaagaaata ttttcaccaa ttattctttc actcgaatca aatagattta gcttcacttc
    27181 gccaaaatct ccctcgatct ctcctgaaat agatttaagt ttaaatttgt tactatctcc
    27241 cacagctttg tagaggctat ttcgaatcaa ggatacattt gaacctgtat ctaaaataaa
    27301 cgttagtttt tttataggct cttgctgaaa ctttgtaaaa atttcaattc taggttgtaa
    27361 tagattagat tggtttgctt gaaactcgtg atgtggtaaa tgtttaatga attctgaatt
    27421 ggtagtgcga caggcaagaa aaagaaaaat tgctattatc cgcccgaata aatttgggcg
    27481 gtataatagt ttaattttaa atatcaaatt actattgaac tttaaaacat acagtttgat
    27541 ttcttccacc gtgttttgct tggtaaagag ctttatccgc tctttcaatt agttctttgt
    27601 tatttttatc tgttggatag aattctgtaa ctccaacact aatcgttacg cgaagtggat
    27661 ctcctccatt tgggttttca accacttgag cttccactgc ttttctaatt ttttctccca
    27721 tttcatagcc ttcttgtaga tcagctccag gcataacaac acagaattct tctccaccat
    27781 atctagcagg tatatcattt ttacgtgcac atgtaatgag tttttgagca acttcaatta
    27841 agacaacgtc acccgcttgg tgtccatgag tatcattgaa tactttgaat ttatcaatat
    27901 cagtaaagag tagggcaagt tttgttcctt tttttctgca tcgatccatc tcttctctta
    27961 atttagattg gaagtagtga tgaattttta actgggtcat catatccaca gttgacaatt
    28021 cataaagtct agaattttca acagcgacac ccgcaagact cgcaagaatt gtaagaaatt
    28081 ctttttcact ttcaaggaag tcgctcattg tcatcttttc acctaacatg agtaaaccgt
    28141 ttacttttcc ttttgcgttg agtggaatga tcatttcagc acctagtgct ttaaaatatt
    28201 taaccagttc atcatccgcg aatccattgt ctgttagttg acgaatcgtc attgcttttg
    28261 gcttttcttc aaagaattga acgagaggag aatcgatcat aattttcatc ttcatatctc
    28321 tatcagcgat atcaaaacct ttatggctag aatccaatgt aaagtgatct gcatccacat
    28381 ctggtgcaag ataaattgct gcgtttaagg tctgcaattg agcgagacaa atatttaaga
    28441 ttgcatcgat cagatagtta tagtctaacg ttgaatttaa agctttgcta atttctaata
    28501 attgcttttg gtcatagatt tttttctcgt aatgttctag ttgctcgaat gaacctgaat
    28561 cttctttttt cttactcaac tttttttacc tcttactaaa tttgcacaaa gtgtatatac
    28621 aaatgtataa actacaagaa catatttttt aaaaaagaaa aaaacaattt ttgatagaaa
    28681 acttttttaa tctgttgaca tagatacatg aaattataag gtgacatata catcgcgcgg
    28741 tggagcagct ggcagctcgt tgggctcata acccaaaggc cacaggttcg aatcctgtcc
    28801 gcgccattta ttttccttcc tcttccaaat aagaatccaa cccaatttga attcacaaaa
    28861 aaccattttc aaaacaaatg caaatcgtat aaagccatac taatttgcct gctagtattt
    28921 gttgcttgct cgccctttat aatctcaatt ttctttttac tgcgagggtt tctctcattc
    28981 tatctatttt aagttttgat gcggttggct taattcctgg gcgcgtttga atgctgaata
    29041 gactgcattc atcacagagt agtgcattcc gttttcttcc aatttttcca atccaaaaat
    29101 tgtggttcct cctggtgagg tcacacgatt tcttaaaaca gatggatgag tattttcctt
    29161 ttctagttct ttttcaagaa gcattgcgct tccttttact gtctggatgc ttaaacgaag
    29221 tgcgtctgca taggttaatc ccgattttac cccaccttct gctaatgctt gtataaaact
    29281 aaatacaaaa gcaggaccgg aaccagataa accggtaact gcatcgattg ctagttcaga
    29341 acttagctct actaagagtc ctaacttatt aaaaatttct tttatatctg gataagcttc
    29401 tttgtcccca atataaccca tacatccttc ttgaatttta agaggtagat tgggcatgat
    29461 acgaactaca taggaattgc ttggagcgtg ttttttgagt gtgtccaaat tgatcccggc
    29521 agcaatggag aatatttttt taggctcctt tatttgcttt aatgtatcta caatcttgtc
    29581 tggcttgaca cacaaaatca atcgatcaca ggaatcattg agacttgaaa aatcagattc
    29641 aaattttata tcatttcttt tgctatatgg atcatagcag aggacagaaa aatctgattc
    29701 gattgccttg taaataggat aacccatatt gcctaaacca ataattccaa cttttttcat
    29761 tttaatatcc tttctccaaa aatggcagaa ccaactcgaa tataatcact tccttcctcc
    29821 gccgcgatca aaaaatcacc cgacattccc attgatagtt ttgcattcgg tgcataatct
    29881 tttcgaattt gattgagttc ttgaaatacc ttcctagttg tgatcaattc gccactagaa
    29941 ggacccatcg tcataagtcc atggaaatga gcaaactcag actccaaagc agaaagatct
    30001 ctgagtattt caattaaatc ctttctaata aatccagact tagtatcttc tagagttaaa
    30061 tttgcttgta gaaagtagta aatctttgct tttctttggt ttgcttgttt gattaattct
    30121 tgcagggaat ttttcgttcc aactccatgt gtataagaaa aaaaaccgag taatttacgg
    30181 atggttcctg tttgaaccgg accaatatga tgcaattcta aatctgggta aatgccagtt
    30241 agcccttgaa acttatcaat gccttctttt atttgatttt ctccaaagat acgaatgcct
    30301 ttttctaaag cattcgtgat tttttcaatc ggttgttttt tggagacagc gattagacta
    30361 ggagtgttct ctggtcgaac tttctgaatt tggtctaata taaaatcgta gttttctcga
    30421 atcaaaatta ctttttagat tttgtgatca ttttttctaa gcgataaaca cgattggcta
    30481 gacttgcttg tttagatttt ggttttttaa ttccaaattt cttttctaaa taagatactc
    30541 ttttttcaag tccgcttgtt ttcacactat tacgtgcttt gttcttttta tgatgatgtt
    30601 ttctgccatg cttgtgtttt tttggggaaa tcttgggagg tttttcacta ttgaaatttg
    30661 ttttaggaga atcaattaaa acgttagtag caaaattatc ttcattgatt ttagtttttg
    30721 cactatcctt gggtttgata atcggtggct catctagacc gattggagtt gtatctttca
    30781 ccggtgggga aagaggctca tcaaatttgg aagttgtctt tgccgtctct gtaggatgtg
    30841 gcgttgtttt cgtttcatca ggaacaagcg aactattttt tgattcatcc acagtattcg
    30901 aagtggtgga gttttgaacg ggaggcacag tgtttcgttc atgtttttca aatccatctt
    30961 tgttggcagc accttgaaat tttttaacaa cttctgccca atttacaaca gtaaatactg
    31021 caatcgcaga gataatcagt actatgatgg ttgtaagaat gattttaaga ctattttgct
    31081 tgctcatatt ttatttatct ataaaaagcc tttttaaata cagtattttt tattgtttct
    31141 aacctaaaat tccgatttta ttacaattct aataaccgta atctttggat tcccgtgtaa
    31201 accgctgcaa ggacaaaaaa gtgtttagat gaaattctcc cttttatttt tagaaatgga
    31261 cttttctttg ccgtttagta aaaataaaat tcatttttat attcaaagga tcagttttga
    31321 aaaaaggatt gtaacactat ttattttcct ctctacaaag ctttattgct tcttactaga
    31381 atttattaac cttgaaataa aaccgatgta attgagtata gagaattcta ttttcccttt
    31441 tttgcatttt tgagaaacta tgagaaagtt tacaatgaaa tcattgattc tatttaaaat
    31501 tatttttctt cttggattta ttttccagaa ttccattgat tctgaatcgg aagatatgaa
    31561 tcgaatcaag ttgattcagg atgcagttgc aaaaggggat cgatataaaa ttcatccagt
    31621 tgaattattt gatggggaga tgccttggag agtggtgaag gggacttctt ttttagataa
    31681 aactagtttt gtttctaagt ccccgaatac agaagaattt gcacttgaat ctaaatttta
    31741 ctatccctta aaaactccgg aaaaattaaa atcacttcaa atctattcca atattgaaat
    31801 tccgggtaga gataaatact ttatttctcc agaaatcgaa accgattttc cagttggaat
    31861 tccatctcgt atatttcttt gggtttattc taataactat gatatgagtt taaaagtaat
    31921 tctatcaaaa aaaagagcaa aggatattat cgttgatttt ggttcccttc gatttaatgg
    31981 ttggagaaga ctagatgcaa aagtgaattt ggatagaaaa atggaaagat taaacttgtc
    32041 taaaaaatca gtttattcta tcaaaggcat tttagtggaa acatcacctt ctcaaagcaa
    32101 agggagtttt tttctattta tagatcaaat gggagtattg cttgagacac ctgaattgta
    32161 tccaggttcg gaagtcccag atggttggga attgtattag tgaatatgct ttatcattca
    32221 cgtttcgcag cttgacctct taaaaaaatt tcttaataaa ggagataaat gtgagtaaac
    32281 tattttcaga agaagaattg gaacaaatta aatccgctgt gaaagatgcg gaaaaaacaa
    32341 cgtcagccga aatagttcct gttttttatg agtcttgtgg tctttatcca gatacatatt
    32401 ggaagtccgg tatttttttt gctgctgcct attctttttg ttatcttttt tataccaaac
    32461 tcacgaatac atggggcgac tctctttcta agttttttgt tatgcaaatg actagcggat
    32521 taattggagt agccgctgtc tatctaatac ctgcttggaa aagactgtta acagataaga
    32581 tgtttgttaa gcaaagagtt cgagatgtag cctatcgagt gtttttggaa gaatctgttt
    32641 ttaatacaaa gacaagaact ggaatgcttt tgtttattag tttttttgag aaagaggctc
    32701 tgatcttagg agatgaagga atcaatcaaa aagttactcc agatgtttgg gaaggaattc
    32761 tttctcaatt gatacagggt atgaagagtg gtgagaagac caattctatt attgctgcca
    32821 ttcgctcgat gggaaattta ttaaagcaat atccaataga cgcaaaggat acaaatgaat
    32881 tgagagatga tttacgatta ggagatacaa aatgaaaaaa ttttctatta tcattttctt
    32941 attattacaa acagcctcta ttttcgctct agatgttcca tatctttcag gaagagttgt
    33001 agatgaagca ggaatcttat ccgaggagac aaagtcctct ctagaaaaga aattaataga
    33061 acaagagaaa aaaacatcta accaattagt ggttcttaca attccttcct tggaaggaga
    33121 agttttagaa gattattcaa tcaaggttgc tacaacttgg aagcttggtc aaaaaggaaa
    33181 ggacaatgga gtcttgcttc tcatagctaa agacgatcgc aaacttagaa ttgaagtggg
    33241 atatggtcta gagggaacac taacagacgt tatgtgtagt cgtatcattc gaaatgaaat
    33301 tactccttct tttaaaaggg gtgatttctc tggaggagtg gaaaaaggag tcgattctat
    33361 ccttgctgtc attgatggga gttatgtaaa tacatcgagt gattcaacta caagcaacga
    33421 ttctggaaag gatacaaata ttgaaggagt ttattcaaaa tttatacatt cagttggaga
    33481 ttcagatatt ccttttttat ttagaatccc tttcggactg attttcttta ctgtaattac
    33541 tccatttact atactcactg cagtcactcc ttatgttggc tggtttttat atttcttttt
    33601 aatgcctttc tatggaacct ttccggttgt tatttttgga aaatggggag ggctctttct
    33661 tccaagttat atcgtaatca tgttcttttt gaaactttat tttggattca caaaagacgg
    33721 aaagaaatta gcagaaaaaa tagagcaaaa atatggtgga tccggtgggt cgggtggatc
    33781 tagttcaggc tctagttggt cttctcgctc gagtggtgga ttttccagtg gtggattttc
    33841 aggtggagga ggaagttttg gtgggggtgg aagttcggga agttggtaga cgatagtcgt
    33901 tcaatgttag atattaaaag attaccaccg aggacaccga ttaaggaacg ataaaaaaac
    33961 gatgaagaat attcgttctt tcttcggtgg caataaatat tcagattaaa ctgacgtcag
    34021 ttgaatgacc taatacgaaa acttcttttt cttattggaa acgaaatcat agataatcat
    34081 ttgatttagt tcatttggat gcgggtaaaa aattcttcct tgtgacattt tggcaaactg
    34141 ttggataaaa cttttttctc caaaataatt catatcccat tctgacgtta gtagaaatgt
    34201 attgataatg attccatctt ctgtgcattg ttttacttct gagagtgctt cctcaaaatc
    34261 agacggcgca ggtggatagt tgatatgaag gattgaaccc ttgaaatgag cagtaggcgc
    34321 tccatccgtg attaaaaaga tctgtttgtt tttagtttgc ttggaagaga gtgcttgtct
    34381 tgcgatttga agaccttttt gtagattcgt aaaatactga ggaactctat tttcaatgtc
    34441 ttcttttttc attttagcaa aatcaaattt taatttgata tagggattaa aaatagtaac
    34501 aggatagggt tgtagagtag ggacttttgt gattggaaaa atttttgcag tgcttccaaa
    34561 accaacaaca gtcagttttt catctttgta ttcatttcgg atgagtgagt ccattgccat
    34621 tgtcactttc ttagcgttgt aaaatcgcat ggatcgcgcc attgacccag acatatcgag
    34681 taaaatcacg gtagaagatt ttgcattgcc tcttgcttga aaaacatcta aatctccaag
    34741 agaaggaatt gtcccattcc ctcttagaat gctattgata atacttcctg gaaaatcaat
    34801 attggaaaca ttatctccaa attctaattt ttttgtagaa gacgttaggt tctcagtgtc
    34861 cccaaattca tttgtgacat gcgatccaga actagaatct gtctttaatt ttgaaaagat
    34921 ttcaatgagt gcagttttac caattttacg aatggatttt ggggaaagct caattccacc
    34981 ttcagcattt tcttgtatat caccagattc ttgtaacagt tttttcaacg cttcaaagat
    35041 tctttctctt ctttcaataa attcattgta actttcagga cctaaaaatt tagcgagctg
    35101 ttctaaatta aaattgtata gatcaccaga ttgaattgcc tcttctaatg ctctatttag
    35161 ttgggttagt tcatctaaat tgtctaaaat atttcctgct tcttttggag ttggaacttt
    35221 tttaccagtg aaattatatt gtttgattcc attactaatt ttatctatat cttgcaattt
    35281 ctgaatcagt tcatctagac ggggttctat tttcttttgg gcagctaaat cccatttgag
    35341 tctacggagt aaatctggat ttttcccatc gattgcggct tttagttttt gtaagatgtt
    35401 ttcttctttt ttaaacttct tctctaaatc agaacgaagt gattttaaat ctttttctaa
    35461 ttgttcagat gcaggattta agttaaaagt ttttaaaatc tgatctatca ttgcttggat
    35521 ttttttctgg aagtcagcaa ttaaatcttc catcccgcct tcttttagaa agagattggc
    35581 gggaatgcct ttttcaatca tcaaacgcag cgcttcttcc agagatatat catatttcat
    35641 gataagatca gacagaatgg acatgagttt ttctttgtca aagttcttgt cgtcatctgg
    35701 aaagtattcg ctgtattcgt atgttatcat tggattgttt cttcttgttt gatgcttgca
    35761 ggataacaaa catttttgtt taaatcacag taaaaaattt tagcggatac ttgtaatttg
    35821 ccttttccgt ttagtgtaag agtgttgtct tctacttttt caaagtattc tggtttgcca
    35881 agaattgtat ttcccttaaa cgttagttct gttgggttga ttttgatttt atcttcagag
    35941 ctgagtttga ttttattcgg tgcatctttt tggattgcat aaccttttgg gacagaaact
    36001 ctcatgatgt aagagtctcc actttttttg tcaagttgaa gtttaattgg atgatccatt
    36061 gccattacct gtatactaat gaatagaaat aaaataattt ttttcataca ttctatcttt
    36121 tatcgattcg cttttttttt ctataaaaaa attacctttt actatcctaa catcctattc
    36181 ttttgagggt gtaaaatgga ttgacagaag caaaggctta gaaacaaacg ctatagaaat
    36241 ctgagaatag aaagaaaagc ttagattgag agtcccaata gtttagttca gtggattggg
    36301 tgtagatttc tgtcttaatt tttagtctcc gcaaaataga gctgatttag ttttcataca
    36361 ttttaaaaac tgaggctaaa tacaaaaagt tgattagaaa ttcttaaatt gaattgagaa
    36421 gattttacct agtaagcttt ccggagttag attaaacctc ttttttaatt ttctacactt
    36481 gttatggata aatatttttc aagatttatc aaaagaattt agatagctcc ctagggaagc
    36541 tgattggctg gatcggggaa tggaacggtt aatagatgtt tggcgaattc tgaaactttg
    36601 aatataatta taagattaga gactctaaaa cttttaggaa tagtcgagat aagtgaaatc
    36661 actagaagca cattatttgg attgaccaag cggtaaaccc ttgttatcct gctctctatg
    36721 ttactaatta gaaacatcct gtatctagtt ttaagtattc tactttttca ttgctcggag
    36781 tcggagtata aaaatccttg tgatcctacc tctcaaaatt ttttaaagtt actccttttt
    36841 aagagattta cctcggatac tgctagtttg tgcggtttga atacttcgaa tcttttcgga
    36901 acaaactctc tttcaaattt tagttatgca cctgatttat attatgcaaa tggtgcttcc
    36961 atttccttat ctcctacagt ttcgagttca ggactaacgt ttagtattag tccttcactc
    37021 ccagggggta ttagtttaaa tacaagcact ggtaaaataa caggatctta tgttggttat
    37081 ggcggatcta gttttaccta tacggttcaa gcaagtaatt cgagtgggtc tatcagtact
    37141 agttttaaat tgagtatttt tggaaagaat ccaattcaaa ctatgcagtc acagtgttgg
    37201 aatgtgagtg gaacgttaga tgcaagttgc acagcaggca cttctattgg acaagatggt
    37261 cagttaaaaa aagggacgac tcccagtttt acaggaccta gtcttgttgg ttctactgat
    37321 tatataacta ccgataataa tactggtctg atctggaaat cctgttttga aggtagatcg
    37381 ggagcgacat gcatagggac acttagttct ttaaatgcaa caactgctgc tagcacttgc
    37441 acttctttaa attcaggatt aggatatgca aatcgaacag attggagact cgcaagtttt
    37501 ggggaaacca atacaatttt aaatcatatt ggcacgagcc cagctacgtt tacatcgaat
    37561 tttccgagtg cgagtggggg aggaacttgg tcaagcacag gggatgtttc aaatagtgcg
    37621 aatgcttggt atatttcatt caccgatgga acgataggct ctacagtcaa aacaaataca
    37681 aatgatgttc attgtgtttc tggctcaagt cttccagcag ctcaatttgt agacaatgga
    37741 gacgatacaa ttgtagatat aaatacaggg ctgatttggc aaaaatgcac gaatggattg
    37801 tccggctcta gttgccagac gggaactgca acacaaatga attgggcttc ggctctcact
    37861 gtctgcaatg gactcagttt aaatgggaag acatggcgtc ttccaaatat caatgaacta
    37921 aagacaatta tagatgtaac aacgagtagc ccatccacga atacaacttt ctttcctgtc
    37981 actcaattag gaaactattg gacatcttcc acctatacga caacatctgc cgcttgggtt
    38041 gttcctttcg gtgctagcac attttatgct acaaatctat caaagtcgag taacgcatat
    38101 actagatgtg ttacaaccgg accttaagta agtcctgcga cgatcttttc tgaaacatcc
    38161 caaagtttct ttgcaagatc ttcgttattt gcgtcctttc ttggagttgc aatattacaa
    38221 tcagcaaaat agtttcctga ttcagatatt acatttggat tcgttgcagc ataacactgc
    38281 gtagccgctc cttccggtat tgatttaaga aatagatgtt ctcctaaaga gaaaatagct
    38341 tgtaagacag gattcatatt tcgagcgaga tttgttttga taacaccagg atgaactgca
    38401 taggctgttt ttttagttcc tacaaatcgt ttggcaagtt ctttcgcaaa cagtagattt
    38461 gcaaatttag attgtccata aaatgtccaa gcactgtaat atttttctcc agccagattt
    38521 tcaaagtcta ctccttcttt tggagcgttt cgatgtgctt cactgcttaa cattaccact
    38581 cttccttcgt ctgttaattc tccgactaga cccgttacta atagaaaatg tccaatatga
    38641 tttgtaaaaa attgcaattc atatccatat gccttttcca gtgagggtaa tgccattata
    38701 ccagcattgc agataatcac atctagcttt aatttttgtt ttttaatttc ggatatacaa
    38761 tttcgaacac tagtcgggtt tgatagttca cattctaatc ctatatgaga attcccgctt
    38821 atttctttca aagcttctat tgctttttct tttgttctag ctgttccaat gatttttgcc
    38881 cctctaagtg caagaacacg tatggtctca ttacctagtc cagaattgca tcctgtgact
    38941 aatattgttt tgccgcttag tgaaatattt tttgtaactt cttctgcagt cgtgtcataa
    39001 ccaaatccat ttttaccttt tgctttaaat aatgctatca gtgacatatt accctcttat
    39061 tgatttctaa ttatacagtt tttttatgct ttgagaatgt aaatcaaaac ttttattcaa
    39121 atttctgttc caagtttatt gattatgaga atgcttagat tgattttcta aaaattaatt
    39181 tcattctaac acaaaacact gctaagatgt ttcaatagtt atttagagct tacgactatt
    39241 ttagattgat tcttctgatt cctgaattaa tattgtggaa ttataatata tatgaggcga
    39301 tcctatgaag attacaaaat ccagcttact tctatttatt attttaatga ctagttttac
    39361 aattagttgt aaaggaaata gagaaaaagc aattcaatac aatacaatga tacgatagtc
    39421 gatgaactga acaagttatc cactctcgaa gattcaatca ccaaaagcga aggagcagaa
    39481 gctgataaag caattgaaga atttaaaaaa gctgttccgg aagctcgtaa aaaaatagaa
    39541 tccattggta aatttaaaga cagtgaaagt cttgaaaagg cagctttaga tctttgcgat
    39601 ttttatcaag gtgtgattga tggaaaatac actgataaaa cgcccgatga gataaatacg
    39661 aaagaagcag aactttatgc agcattgaag caagctcaga aagattttgc ggaaagattt
    39721 aaaatcgaaa ttcaaaaata attcctttga aagaaagaca atgagtgcga ttttaaaatc
    39781 aaactcatgt cacttaaata aaacagtata ccaataagaa tctctgtctc ttaacttcca
    39841 tataaatcca tctaatacat aatgagttgc ttgtgggagt gttagaaggg tgacaagtat
    39901 gctgagtgtt tctttacttc gtatcgttgg aagagatata aatggtttaa aaaattctaa
    39961 atgctctcgc caaataaaac ccgcccagaa tccttcttct atataagcaa ataaaaataa
    40021 ggtcagaata aacattggaa atgtgaattt ggtaaagaat actttgtaag aaaaacggtt
    40081 tagaagaagc ggtgaattcg tattatgcgc ttccttattt ccatagaccc aaatcagtgc
    40141 aatataggga attccatgtg ttacaacatt tgtaattgta aatacaatgt cttgattgaa
    40201 gtaaacaatc ccaaaatacc aagaaagagc tgttcctagt aataataaat ttttaccgat
    40261 tgagaaatat ttttttgtaa tgatttcctt tagttctttt aagaggtaaa ttccgattat
    40321 ccccaaatag aaataaaaag agattctctc tacccagaga ggaagcccaa tcaaaaagtc
    40381 tccatctaca aaccagtgaa aatttctagg gagatttgca tgccagtaga taacaggata
    40441 aagagtggag gagtagagta agactgcttc taatttctta gaaaatccaa aagagtcttt
    40501 cgagtaaaat cttaaaaatc catattgctg cctgataaaa tgaaatacag ccgaataagc
    40561 aagaactctc caaaataact tggaatcaat taaatgaaga attacaccta gaaaaaaaag
    40621 accgataggc agtaaggtta aaagagtttt attttcctgg aattcttttg gattaaaata
    40681 agttcgaaag agtgtgctat aaacatgcga tacatcaata caaagaataa aagacaccca
    40741 aatccaaaga ggcattttga ctgtatctaa aaaaagatca gagaaaagat agataaatcc
    40801 aatggatagg aaaggaggag aaagtataaa aactaaatcg aaactttgat tgtaaatcca
    40861 attcttttct ttcatattga tatttgtttt aatatttttt tagctgcttc acaacctcta
    40921 tattgtgctt cttcaaagat ggaaattcca ctcatatcag aatttgcaaa ctcgattccc
    40981 ataaaggatt ttaacaattt ctctcttttt tcactccaaa gaaaattaat tccaggactc
    41041 accatcccat gaccccaaat ccataaatct atttctaaaa tcgtgtccgt gatccctgcg
    41101 tgcatttttt ccaagtcttc taaaataata tctacccaat cttctttgga tcttagataa
    41161 gcttttaatc tttctgcttt tggttcttcc tcatcgaggg gcaaataata agtaaccact
    41221 gtttcttttc gatgaagcgt taagtcttga tgatttgcaa caatataccc aagtgattta
    41281 ctataaaagc ttacattgtc ccatgaaagg tttgagccta aaccagtagg tttttctttg
    41341 agtgtaatat ttgcaatgag ccaaggggta aaatcaagag tattcgctac atcatctttg
    41401 taattcttga ttactttttt tgcagtgtaa cgaggggagg cataaatgat attctttgat
    41461 ttatagcgaa taagggaatt tgtttccgga tgtattgtat ctacataata gaagttttca
    41521 tatttttcaa ttcgataggt gagtgtattg gttcgaattt tatctttgca aatctcagat
    41581 aatttttgca ctagaaatcc atttccagca ggccaggtta atacagtttg agaatcagcg
    41641 ttagccgctt ttccagcacg cgaggcaaaa taatgtaagc ctgcccaagc agagactttt
    41701 ttatgagaaa caccgtaatc atctctacaa gaataattta cataccaatt taaatatcta
    41761 gaggtcaagc ctcgtctttg cataaaatca aacatactgg ttttgtctaa gtctagaaac
    41821 tctttgtctt gggagctttc ttccattgga attgcaaagg caggttttcc atcttttcct
    41881 tttgtgtttt ttaatttttc tacaagagag aagaattctt tatattgttt tttatcttct
    41941 tcttcaattc catgtgcagg aacaagtccc tcctgccaaa tcccttgaaa gtaaagtctt
    42001 tcgtgaggat cggcgcatag atagaattca ttgtaaattg gtaaatcttt tttaaatcct
    42061 tctatgattc ctagttctgt aaaaagttcg cggacatatt ttgcctcact gctaggaata
    42121 ggaacgtaat gcgctccata cggatattca cttacatcgt tttttccaga tttagaattt
    42181 ccgccaacgg atttatccat ttctaaaatt agaaaatctt cgtatttatt ctttttaaaa
    42241 taccatccag cggataatcc agatatgccg ccacctacga taactgtttc tgtttcgatt
    42301 tcattttcag gaattgattt aatattttca cgcagtaaat gtcctgcttt gaaattcgca
    42361 ccagagattt gaattttgaa attgtattgt cttctttgta gaaaattttt taaaccaaaa
    42421 aaggaagaaa atacagcaat acttccaaga cctaaatact ggagaaattt tcttctagtg
    42481 tgcatactct tcccattctt cttcaaagag tcgaactagg atttgattgt ttaatctatt
    42541 tacttcattt gattcaacaa tcatatcatc tgaaaaaatg gtaagtgttt ttgcaatgga
    42601 aggtgtaata tattttagtc ctgttggata tttttctgga atcgatattt tatcttttga
    42661 aaataaaata tagccccaat ctccaaaagc aggaacatag acatgatagg gaagggtatt
    42721 tagtccaaca gatactaatg tattgttgac acaccaaaaa cttttttttg caacataagg
    42781 agaagtgctt tgaattactc caactccatt cgttgctaac gcatttttta gaattttata
    42841 aaaagagttc gtgtagagtt ttccgacaga ataattgctt ggatctggaa agtctacagc
    42901 tataaagtcg aattttgttt cgtttgtttt aagccaaata aacgcatctt cattgtagat
    42961 ttttactttt ggagaaagga gtgagttttc gttcaaggca gtgagtgctt tttgtttaga
    43021 aaaaagtcct gtaatttctg gatccaaatc aactaaaaca atttcttcta cactatcgta
    43081 ttttaaaatt tcacgaacag caagtccgtc tcctccgcct agaactaaaa cttttttggg
    43141 cttgtctaaa cttgcaagac caatatgaac taacgcttca tggtatctat attcatcttt
    43201 agaactaaat tgtaaatttc catttaaaaa aagtcttaag tcttgcttgg atctggttaa
    43261 tacgattctt tggtatttgg aatatttaga ataaattact ttatctggat agctagcagt
    43321 ttctgcaaaa ctcatgatct ggtttgaata aacaaaacca atgaataata agatgagtcc
    43381 agtaaacata gcaagcttta atgtctttag aaatggaatt tctttttcaa aaacataaag
    43441 agtccaaatc cctgttaaaa cattgaagat tccaaataga aaagcagttc gcacaagtcc
    43501 caaatgagga actaatagaa gcggaaataa gatagatgcg aataacgcac cgatataatc
    43561 aaaagtaaaa attttagaaa ctagatcact aaattcaaaa tgagttttca agattcgcat
    43621 cataagtgga atttcaagcc ctacaagcat tcccgttata gatacatctg tatagagtag
    43681 aattcgaaaa gatgctacat gctcaaaagc aagaaagaga agggcggcag aagaaccgcc
    43741 cacaattcca atgagaagtt ctacttgcac aaagattcca attaaattct ttcgaatgta
    43801 tttggataag aaagaaccta tccccataga aaagagatag gctcctataa tcgttgaaaa
    43861 ttgagttatg gagtctccga gtagataact cgcgagcgat ccagcaatta attcataaac
    43921 aagtccacat gttgcaatta tgaatactga gaatagtaga acataggaca ttgagactaa
    43981 ctatgaattg ccgctgctat tatgattgcc attgcaatgg tgactgcact ggtcgtaacg
    44041 gctaatggca tattattctt ttgcgcaatt tcttcccata ggttacctgg tgtgatgcgg
    44101 tcaaagatga taaaagagat tgcaagaaca ataatgccaa ttcctgaata tagaaaagca
    44161 ttgattatat ttttgatact gattaaatct gttaacataa aattctcctg aatttattta
    44221 tgatacgagt ttggtcctct tggtccccat ttgcccggac taaataggtc cgaaacattc
    44281 cattgtgtat aaccaccaag aattaataaa atacaaagta tgattccata ccctaaaaaa
    44341 tttcttgagt tcattcatcc tcctcttccg agtcatctga gctatcttgc gaaaaattac
    44401 tatttgccca acgtttattt tcaaaacttc tagatctaaa ccaaacataa aatggataca
    44461 ggcagagaag aaattctgct aggaaaaaat ttgcccagat tgtagatcca gttttaactt
    44521 caatggaaag tttcgttgct tctagtggat tactcggatt agccgcacca gtttgcatag
    44581 caggtgtagt catctcaata tttatatgat atttcccacg ctttactcca gatatgtctg
    44641 ctgtagtaga agtgcttcct tctgaccaag atccatcact atcgactcca gagtagtatt
    44701 caattccttt ctcaaagtct acactagcac ccgtttcgtc gtttactaaa tcacctgaaa
    44761 tttctaccca actattgcta acgggagagg ataggtttat atcgatgctt gtaactctat
    44821 ctttgatttc aaattgaggg gttacttttg ttttgccttt gtcttcaatg gtaaaatcca
    44881 tttccccttt gtagactgtt tccgttccga atccaataca gattcccaat tgtaaaagga
    44941 tcattgcatt aaaaatccat ttccagtatt tctggatttt ctttttttct tctgcgttga
    45001 tcggacctgg ttggttagga gctatgccaa ttaaaagtgg cattggttcc gttagttgaa
    45061 aggctgtttt aatttcttct gagtttacat aggttccaac agaccaaacc aattcttctt
    45121 ctgtggattc gactccaagt gtttcgggag gagagataaa ttcttctagg ttgacagttt
    45181 ctcccattcg aacttgccaa taaaattctc cgattacata ggtgacagtg gcagttcctt
    45241 tgttgaataa tgtataatct cgttggttgt aaggaacata gactgggtcg gcacttcttc
    45301 tgatttttaa atggtctttt atcatcgtta caaacgacca atgtccattg aactcagaaa
    45361 gaaatcgaaa tcctttgagt ggattgaata ataaatactc actccaagtg taaccttctt
    45421 ggtctgttcg ctccatataa ccaatggttt cccaaagcgt gccaaataac gttcctcttt
    45481 gaccgagtgg aatgattgtt cttctttgga tttttttggt aaaggcatct actactttgt
    45541 aattttcatc gctagtatct ataatggagg agcaataggc gcagaccaca ttgattgtcg
    45601 ctccgatcgc acggatgtta acagagcctc cgcaacttgg gcagctaaac gtttttactc
    45661 gggtagattt agtgtttgtt ccttccatcc taccagccct ctatttgtct cagattttct
    45721 aattttaaat cttctacttc aaagtatcta ccaacaaaga gatgttgttc ctcttcggca
    45781 tagtcaaagt tcgcaaaacc ttttctctct gatcgcaaat ctacacttaa ggattttctg
    45841 cctactaaac atttgaatgg aagttcacct tcgctacctc gacaggttac ttcatgcaca
    45901 tcgtccacag agtattctgt ttttttgatt tctaaaatat ttccaacttt taactcggat
    45961 ttatcaggaa agtctttgaa atctctttct tcaaagctaa tcatataaaa gccttgtgca
    46021 tcagagagcc agccatcttt tccactttta aaagataaat accattcatt ccaaactcca
    46081 ttggagtagc caatcttttg tcttccaatt acctcgaatg ggtcttttcc cacaatacca
    46141 gtggttccga tttgaatcgg gctcatgtcc tctggcatat cgctcatctt gcctaggagt
    46201 tctaagtctt tgtcatgtcg gacgattgtg gatttgcagt atttacacac tgccataaat
    46261 gcaaatctag attggaaggg aacctcagct ccacacgaag gacaagatgc tttcattaaa
    46321 atttaatatc cccattttct aaagtatgtc aacacatatt accaatgcac cgtgtaaaaa
    46381 catgaggaat ggggaaaaac cctctatgct tttcgtggtc tagaaaaact gttagtatga
    46441 aatttctgca ccacgaagaa cacgaagagt cacgaagcta tgaccctaag actaccattg
    46501 gtattagaat gatttgagat tatgtgtcgt tttggtctgg aaattgggat attattcgtt
    46561 actagtatgg ctcggtcagg aataaaaagg tattctctct tttgtcaatg ttgagttttt
    46621 ttattctttg ttctattcaa aatccatttt cagtataaca accactatac caagactata
    46681 cgaaccacta taccaagtcc tttttttatg gtggggaggg tagaactaat ggaaatcttt
    46741 tccgattaaa caatgattat tttactctaa aagcactcat ttattctgtt atctatacat
    46801 ttacatagtt cttaatccca gaggtttcat ttttcaaaaa ttcccttgtt tttgttgttt
    46861 ctattatcaa agtggctcta caagaaggat agccaatgca agaacaaaac accaccacag
    46921 aaatcaaaac tccaaataca gaaatggaaa tcctgcgcaa gcattttcct cattgcttcg
    46981 ataagaatgg gaactttgat ttggaaaaat ttcgtcgtgg tctttcagat aaggaaattt
    47041 ctttctcaac tgagagttac gcgatggatt ggctaggaaa atcttatgcc cgacttttgg
    47101 caagtgatcc ggcaaccaca cttctcaaag cagacgagac acacaacgca aaaccagaaa
    47161 atgccaagtc agaaaaccta ctcatcaaag gcgacaatct ggaagttttg aaacatctcg
    47221 tgggtgctta ttatgaaagg atcaaaatga tctacatcga ccctccttac aatacaggca
    47281 gtgatgggtt tgtctatgca gacgatagaa agttcactgt cccggaactc gcccgacttg
    47341 cgggcataga agaagaaaag gcaaaacgca ttttggagtt cacgcaaagc aagagcaatt
    47401 cgcattctgc atggctcaca ttcatgtatc ccagactcta catcgctaag caactccttc
    47461 gagaagatgg agtcatcttc atttccatcg acgacaacga agtcgcacaa ctgcgtcttc
    47521 tcatggatga gatttttgga gaagagaatt ttgtggcgaa tatttgctgg aaaaaaagat
    47581 cttctcccga tgcgagaacg gtaattggag ctatccatga ttggctagta tgctacaaga
    47641 aaatcggaga aaattcacga gcaaatattg ggaagatgcc cctaaaccaa gaacgtcttg
    47701 attcatacca aaatcctgat aatgattcgc gaggtccttg ggcatcggtt gatatgactg
    47761 gtatgacagc tagagctcct aaaagccaat tttataaaat tactttacca agcggaagag
    47821 tgatagggcc gccttccggc agaagttggg gggtagcaga atctactttt tatgaactta
    47881 ttagcgataa tagaatttgg tttgggaaat ctggtgataa tgttccaaga ataaaaaaat
    47941 ttctttcaga aggagaaggt caagttgttc caaccttttg ggatatgagt gaggtaggaa
    48001 gtaatgacga agccaaaaaa gagttaaatg atattctgaa cactccagaa gtatttgata
    48061 cacccaagcc gattcgatta ctcaaacgaa tgattcaaat tgcatcaacc cctaattcca
    48121 ttatcctcga tttcttcgca ggctctggca caactggaga tgcagtcatg caactcaatg
    48181 cagcagatgg cgggaatcga aagtatatat tagtccaaat ccctgaaccc attgatccta
    48241 agaaaaataa aattgcttat gattttgtga aagatttaac ttcgcggggg ggggggggaa
    48301 ccaaatattt ttgaaattac aaaagaacgt ctgcaaagag cagctcagaa aattataaag
    48361 gaaaatatcg atatacctgc aaaaacagaa aaagtaaaat gcgaaggtat ccatgatgaa
    48421 cattgtcctg cttgcaaggg tgaacacaca ataaagctag aagcaagaca agcgatcgat
    48481 ctaaattcca ttgaccttgg ttttcaaatc tatgaaacca tccctatttg ggaagactac
    48541 cacgtggagg cagaagaagt gcaagatcaa aaattatttg atgaaagcaa attgaccaac
    48601 gaagacttac aaacacttct cattacctgg aaaacctatg atggaattcc tctcacccaa
    48661 aatttggaaa aaataagtct tggaacttat actgcttatt atggaaaagg aagactctat
    48721 ttgatgcaca aaggattcca gacagaccac ctcaaagtct tattggaaaa aattgacaca
    48781 gacaagacct ttgaaccgag aagcatcatc tgttttggct accacttcga aagcaaaaac
    48841 ctccgtgaac tttccgaaaa tggaaaaagc tatgccaata aaaaacgtct ggatatagat
    48901 tttatcatca ggtattaaaa aaatttatga gtactccatc cgaaatcatt cttttcaaaa
    48961 ccgcagacga gaaagtttcc atacaagtaa ttatggaaga cgaaacagtt tggctcacgc
    49021 aagaacaaat cagtcaactt tttgagcgtg ataggagcgt gattacaaag catatcggaa
    49081 aaatatttag agaaggcgag ttggatgaaa gagtggtatg tgcaaatttt gcacttacca
    49141 ctccgcatgg ggcaatcgaa ggaaaaaccc aagaacaaga agtcaagcac tacaacctag
    49201 atgtaatcat atctgtcggc tatcgcgtaa agtccctccg aggaacagag tttcgcaaat
    49261 gggcaaccca aaggctcaaa gagtatatca tcaaaggctt taccatggat gatgaaaggc
    49321 tcaaaggagc aggtggcggt aattactgga aggaactact cgaacgaatc agggacattc
    49381 gcagtagcga aaaagtaatc taccgccaag tattggattt gtatgccacg agtattgatt
    49441 acaattcgaa aagcaaag
//
LOCUS       DAOQOS010000032        49011 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_278_length_49011_cov_5.747467, whole genome shotgun sequence.
ACCESSION   DAOQOS010000032 DAOQOS010000000
VERSION     DAOQOS010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 49011)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 49011)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49011
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_278_length_49011_cov_5.747467"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..636)
                     /locus_tag="PLF66_08995"
     CDS             complement(<1..636)
                     /locus_tag="PLF66_08995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64215.1"
                     /translation="MIKSLIIISLLLLGCFQENRYFWNKQDYDKCLQGCKEGEIIGFL
                     VFNDIVNPAPVSATENYFLTFKSILKQEKEPNDSFIQSDSSIVLTLPLASQSLGVFGQ
                     ISSTDVDIYPFSFSQMDFSKRVFNISAIGTKNCSVYTKAITPTTNSSTIDSSFNFVLN
                     VNETIQSFILDSQTATNMYIYCAANSIINNYKILLVRLCQVAHVCKIELKWS"
     gene            961..1857
                     /locus_tag="PLF66_09000"
     CDS             961..1857
                     /locus_tag="PLF66_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012388028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA-like polyprenyltransferase"
                     /protein_id="HMZ64216.1"
                     /translation="MSIFTTINNYGKMVKFSHTLFALPFAGLASVIALLKSNLSTEDL
                     IWKLILILLCMFTARSAAMGFNRYADREFDKANPRTANREIPSGVLSEKSVLSFTILF
                     GISFLIFSYLLSPLCFILSIPALAIILFYSYSKRFTMFCHFILGLGIGISPSGAWVAI
                     QNNLELIPALWSFGLMFHIAGFDILYSSQDVEVDKKLGLYSIPSRLGIPTAFLIAKIS
                     HVIAFVLFVYAGYLAQLEFFYYLFLGITGILFIVEHTLVSPTDLKKIPIAFFHINASI
                     SVILFIGILLDRGKDLYTKFVQ"
     gene            1933..2532
                     /locus_tag="PLF66_09005"
     CDS             1933..2532
                     /locus_tag="PLF66_09005"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004785016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0106141 - flavin prenyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HMZ64217.1"
                     /translation="MKLVLGIAGASGSIYPARFIKKLLEIKGETFLIISPASIRIFRE
                     EYETKVNTAQEILDFIINRWEIKNIQNQIHFRSFQDIGADIASGSNTWDGMVVLPCSM
                     KTIASINAGLTENLIERCADVTLKERRRLILVPRETPYNQIHLRNMLSLDQAGAIILP
                     ASPGFYQMPKTLDDLGDFIAGRIFNLLGVNADLFPKWEG"
     gene            2747..3328
                     /locus_tag="PLF66_09010"
     CDS             2747..3328
                     /locus_tag="PLF66_09010"
                     /inference="COORDINATES: protein motif:HMM:NF017496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HMZ64218.1"
                     /translation="MNTFVLEIPDSIGKIDDEKYLDLCKKNPESRIERSSKGEIIIMA
                     PTGGETGRRNFELGIRFGEWNRKKKLGKFFDSSTAFKLPKGGIRSPDVGFVSNAKWNS
                     ISLEDKKKFPPLCPEFVLELLSDSDSIETSQKKMEEWMDNGCLLSWLIDLDEKKIYIY
                     RKNKPVETLLGLDHILSGEDVLPEFTLDLKEIE"
     gene            complement(3525..3836)
                     /locus_tag="PLF66_09015"
     CDS             complement(3525..3836)
                     /locus_tag="PLF66_09015"
                     /inference="COORDINATES: protein motif:HMM:NF024835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HMZ64219.1"
                     /translation="MKSKEKTISFKSVLNQELKDLKKKKVFDEGYKDFLVFELVYSLM
                     QDDEASIRELSKALNISTRTILKIRNGKENPRIDTLKKILNYLGAEIQIVKNKKVLAI
                     I"
     gene            complement(3833..4087)
                     /locus_tag="PLF66_09020"
     CDS             complement(3833..4087)
                     /locus_tag="PLF66_09020"
                     /inference="COORDINATES: protein motif:HMM:NF017757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64220.1"
                     /translation="MNELIACGIDRLKESEKAMGGKRSARSRPHLKWLVHLAEGALNY
                     KIYCIYGKRENYYNTWIYKEGTKTTCIRIKKSTGCEEGIL"
     gene            4248..5147
                     /locus_tag="PLF66_09025"
     CDS             4248..5147
                     /locus_tag="PLF66_09025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64221.1"
                     /translation="MKKIIIAIIMIIATSIFSQNSEKWSDYQGVMDWETANKKCSSIN
                     MRLPSIEELKVAMKEGSGKQWKKDGLSYWSNAPSGGYGGAYSITIEAAGYRISNQKDG
                     YLNVRCIDGLSIMNKIVKEKISMQWSELQGSGNWEYANIKCTNIKMKLPSIDELKYSI
                     KDDVIKSWEKDGLYYWSSEPFHYIDIKNGSIKENMTVDDKFNVRCTHGLPISKKWIGG
                     YQGDMSWQEATERCANLGLRLPTINELKAAYLSKTMKSWDYNYITRHFWSSTPSPNSS
                     HYTFSNGDKTDLSPSDASVYCIQ"
     gene            complement(5507..6307)
                     /locus_tag="PLF66_09030"
     CDS             complement(5507..6307)
                     /locus_tag="PLF66_09030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64222.1"
                     /translation="MKFSIRKLIEYLHPWGPYIPTSKKDYFLHIILFFDLIFILFLNS
                     YEAFLPKIYSGAAFLFDMFVVTLWGVQVIFRIGRSPDKFEYISTNWYLLPGLVPFPIF
                     RFFLALATLKLMIITYKFIKRGEKDTKQFIKRELNFRFIDLFVDAISDAIFLRSLERV
                     EEVVEKMDFQDLTKDIIAKNKNDLGKVVRETLESKNFMKRVNALPFMQEVTSKVSDEV
                     TEAILETLSTQVVGQMMKEVNLRLLHVMDDRVRKLSLDRITEEDKKKS"
     gene            6566..8650
                     /gene="pta"
                     /locus_tag="PLF66_09035"
     CDS             6566..8650
                     /gene="pta"
                     /locus_tag="PLF66_09035"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF004167.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HMZ64223.1"
                     /translation="MSKSLYITTTEPKSGKSLVSLGIMELLKSGLSRVAFFRPIIKNR
                     DHGKMDPDIDLIRKHYNLQIKYEDTYGLFMDQALELIAADKKSALIDTIMAKCKKLEE
                     EYDFVLIEGSDFSGSTSSFEFELNSELAKNLGAPIMLVLPATHKDNDSVLSDLSLAIE
                     SYTNSGCTIIGSILTRVNPENLTQLKKSYEFKTIDNIPAYFIPENEIIRTLTMKEITE
                     ALEAEVLYGSEHLMRHAYYNTVASMRLENFLNHIKDGTLVIVSGDRADIILGGFLCSE
                     NKNFPRIMGLVLTGGIHPDHNIRKIIDGLDAHIPVIVTKEDTLRTVYKINSIDSKVSP
                     DDRRKISIYLGLFESNVETKKLKEKMIVYKSSVITPKMFEYDLIQKAKSLKKHIVLPE
                     GNEERILRAAEVLVDRDVVKVTLLGKEKEIREKIKKLGLKLKDVNILEPAHSAEFQNY
                     YETYYELRKHKGITIDNAKDVMSDVSYYGTMMVYKGHADGMVSGSVNTTQHTIRPSLE
                     FVKTKKGFSIVSSVFFMCLPNKVLVYGDCAVNPNPTAQELAEIAMSSAETAKQFGIEP
                     RIAMLSYSTGDSGKGEEVEKVREATQIVKAKDPNLLIEGPIQYDAAIDPDVASTKMPN
                     SPVAGKATVFIFPDLNTGNNTYKAVQRSANAVAIGPVLQGIKKPVNDLSRGCTVPDII
                     NTVVITAIQAQE"
     gene            8653..9855
                     /locus_tag="PLF66_09040"
     CDS             8653..9855
                     /locus_tag="PLF66_09040"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HMZ64224.1"
                     /translation="MKILVINTGSSSIKYQLFRMDDKTVLASGIIERIGEEKSKHKYS
                     SINLKESIEIEKKIADHQTGIKEMDELLTDAKTGVIHNSKEITAIGHRVVHGGEIFTK
                     PIIIDPDVIRAIEENIPLAPLHNPGNLSGIFAAQTFFKGVPNVAVFDTAFHQTMPEKN
                     FRYALPNIFYEKHKIRRYGFHGTSHRYVSKTVASFLNKKLEEVNLITLHLGNGASVCS
                     VREGKSFDTSMGMTPLEGLIMGTRTGDIDPAIPFYMADHIKLSYKEIDKILNKESGLK
                     GISGLNDMRDILEKEKTGDKNARLALEMYVNRIRKYIGSYLIGLDGNLDAIVFTAGIG
                     ENSPDIRERVVKNLTSLGIEIDDTKNKTRSKEIREINSDKSKIKILVCPTNEELEIAE
                     QVKEVIEM"
     gene            10246..12942
                     /gene="aceE"
                     /locus_tag="PLF66_09045"
     CDS             10246..12942
                     /gene="aceE"
                     /locus_tag="PLF66_09045"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HMZ64225.1"
                     /translation="MSATPEQSITNSKQVDVDPIETKEWLDALNGVIDAEGTDRAAYL
                     IDRQIEFARVNGVVQPFHAETPYINTIPVEQQSPLPGDQALERKIRSYTRWNAMAMVL
                     RANENSNVGGHISSFQSAATLYDIGYNHFWHAPSEKHGGDLIYVQGHCAPGIYSRAFL
                     LGRLTEDQLINFRQEVDGSGISSYPHPWLMPDFWQFPTVSMGLGPIMAIYQARFMKYM
                     QDRGMIKEENRKVWAFLGDGETDEPESLGAIGMAGREKLDNLIFVINCNLQRLDGPVR
                     GNGSIIQELESEFRGAGWNVIKVIWGRHWDALFARDKKGILMKRLGEIVDGEYQTMRA
                     KNGAYIREHVFNTPELKALVADWTDEDIWNLNRGGHDVHKVYAAYHAAVNHKGQPTVI
                     LAKTVKGYWMGSSGQAMNIAHQQKHMSDIDVKAFRDHFGIPVPDDKLAELPFVKFPEN
                     SPEMQYMKERRNALGGYLPQRRKKADSLKVPSLDAFASLLEATTEGRELSTTMAFVRL
                     LNILLKDKEIGKRIVPIVPDESRTFGMEGLFRQLGIWSHVGQLYTPQDRDQLMFYRED
                     KQGQVLQEGINEAGAMSDWIAAATAYSTHGTMMIPFFIFYSMFGFQRVGDLCWAAGDM
                     RSRGFLLGGTSGRTTLNGEGLQHEDGHSHLWSATIPNCVSYDPTFSFELAVILQDGLK
                     RMYADQEDIFYYITVMNENYTHPALPKGEERNILKGMYSLSKSDKKDAKLKVQLLGSG
                     TIFREVIAAAELLKNDWGVEADIWGCPSFTELGRDINRVNRNNLLNPEGKTEVSHVEA
                     CLKDTKGPVIAATDYVRMFAEQIRPALQNLGRRYTTLGTDGYGRSDTRAKLRHFFEVD
                     KRWVTIAALKALSEEGLIEPKKVSEAIKKYGIDPKKPNPLTV"
     gene            12954..14231
                     /gene="aceF"
                     /locus_tag="PLF66_09050"
     CDS             12954..14231
                     /gene="aceF"
                     /locus_tag="PLF66_09050"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR01348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HMZ64226.1"
                     /translation="MAKLIDVKIPDIGDYSDIPIIEIHVKVGDKVSAEQSLITLESDK
                     ASMDVPSPANGVVKDLKVKVGDKVSKDFLFLVLEGEEGVAEVKAPTPEKPKESAPIAQ
                     SAPPPQKPITPPVQVEVSHDSNGKSSHASPSVRKFARQLGVDVAKVKGTGPKGRITQE
                     DVQNFVKSVMSGQTSTSSNTSGLNLLPWPSIDFSKFGETKRQPLSRIKKLSAANLSRN
                     WIVIPSVTYHEDADITDLEKFRVDTNKQYEKEGIKLTMLSFIIKACVVALKKFPEFNS
                     SLDGDDLVLKQYYHIGFAADTPNGLVVPVIKDADKKGIFEIAKEASELAKLARDGKIK
                     SEQMQGACFTISSLGGIGGTYFSPIINAPEVAILGVSKAAIKPVWNGKEFLPRLICPL
                     SLTADHRVIDGVLGTKFNVYLAELLSDFRRVVL"
     gene            14240..16021
                     /gene="lpdA"
                     /locus_tag="PLF66_09055"
     CDS             14240..16021
                     /gene="lpdA"
                     /locus_tag="PLF66_09055"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HMZ64227.1"
                     /translation="MAEKELKIPDIGDFDEVPIIEVFIKAGDIVQKEQPLLVLESDKA
                     SMEIPAEEAGKILSVAVKVGDKVGKGKLIAVIEVSATANTNQTANTNNPPKADPPKVQ
                     SATAVLPQGSYSGKVDHECEMVVLGAGPGGYSAAFRSADLGMNTILIERYPTIGGVCL
                     NVGCIPSKALLHTAAVMEEVKAMASHGIVYAEPKVDIDKLRHHKETVVGKLTNGLAGM
                     AKMRKVKIVRGLGKFIDANHIEVDLTEGTGQKTTGKKEIVRFQKAIIAAGSQSVKLPF
                     LPVDPRIVDSTGALLLKSIPKRMLVIGGGIIGLEMATVYSTLGTRIDIAEMMDGLMAG
                     ADRDLQRVWERKNSHRFDKVMLKTRASKAEAKADGIHVSFEGENAPSSPEVYDLVLVA
                     VGRTPNGKKIDAEKAGVQVTDRGFINVDKQQRTNVSHIFAIGDIVGQPMLAHKAVHEA
                     HVAAEAAFGEKSFFDAKQIPSVAYTDPEVAWAGLTEEQCKEKGIKYKKGHFPWSASGR
                     AIANGRDEGFTKLLFDEETHRILGGGIVGTNAGDLIGEVCLAIEMGADAIDIGKTIHP
                     HPTLGESIGMAAEIAHGTCTDVPPQKK"
     gene            16504..17013
                     /locus_tag="PLF66_09060"
     CDS             16504..17013
                     /locus_tag="PLF66_09060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64228.1"
                     /translation="MKLEKSSKSYSDLNCIEANYKSNKRFYFLNIEPGQYTIVAGKFG
                     YYRTQSKSPRYNFSMVFDKESREKMKFTVKESELINLGEFLMDESAVSFDQDPDLGRI
                     GEKIFPGFKNNFGKNVQMTMTPGLYQALELPAIGKTFNHDNRPEKLEEIRNEVKEDFS
                     GTEWKIFVK"
     gene            17443..18159
                     /locus_tag="PLF66_09065"
     CDS             17443..18159
                     /locus_tag="PLF66_09065"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HMZ64229.1"
                     /translation="MLFYYKQHPGKKWFASNLLALIGRIASVLLYKVKIISTPDLKID
                     GPVLILAKHSIEIDIPLGYYAMLVAIGRHAWCIMKASLARSRYLGFYWKIGGIPLDRD
                     KPEKSKHMLVYAKKVLYDLDESGLKKGNGNVMVLFPEETTYWQTMGEGQAAGFRFIVG
                     KPAIPLNVVCAGFRYTKGFIRTNLEIEFGPIRQFTKDDDHASFLHDRMVEIAELSNLP
                     YPFSQPKSRAKVLTEASASN"
     gene            18677..21331
                     /locus_tag="PLF66_09070"
     CDS             18677..21331
                     /locus_tag="PLF66_09070"
                     /inference="COORDINATES: protein motif:HMM:NF019224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1554 domain-containing protein"
                     /protein_id="HMZ64230.1"
                     /translation="MKIRNYFFALFSTVLIHLTCSLSDKPKVLGWIPFKPPTTPAQIF
                     INSPAIKATAEDGISLQIRFSLNKSPSSQVSIQITNSNPNEASISTTNLNFTSSNWDT
                     VQILTVTGIDDLYVDGDRKYNINFKITSDDLEFSNLLLDPIEIINKDNDTPGVQVTPR
                     TGHLTSESGATTTFQVFLSSAPTATVTIPTIASSNTLEGTVSPSTLTFTPTNWSIPQT
                     VTITGVDDSIDDGNQNYSIVFGTTTSSDPVYNNFVTTNLNVINADNETGSGITVSGLS
                     GNTTEVGGTATFNVILNTDPTSSVTIQISSSNTNEGTVSPNSLTFNSGNWNITQTVTI
                     TGVNDLIQDGNVSYNIIIGGVTSANSNYAGLANQTLSLSNTDNDTAGYTINTTNPFLV
                     TDGGQITSSIQISLNSQPTSNVTIPIASLNTAEANVSVSSVTFTPSNWNIVQTILVSG
                     VNDGTVDGNQAFTIDLAIPTTSDSVYAALNPANQSGNSCDNDGTGVLISVCRTNSTLT
                     TTESGGALTFYLLLSQSPTANVNIGLSSSNTSEGTVSPATITLNTSNWNTFSGSNLIT
                     VTGIDDSNYDGDISYNLNFATSSSTDLVFNGINLSPIGLVNQDNEIYFNVSAVTGSPM
                     VENIIGGTASFTIALNSAPSGINTVSIGISSSDATEGTVSPSSVVFNSGNWNTPQTIT
                     VTPVDDTIADGNISFTILTASAVSGDIRFNGRNPADRTLVTNDAGEKRTFLTFTTYNG
                     NLGGIAGADTKCNGDTNRPSITPNTYRALVSVNTTRTGNPLAGSWSFAANTTYFRTNG
                     TTNVFPSDAGSIFSFGGLTNSFSGASDEYWTGLNTNWTVGSNCTAWTSSSNAISARYG
                     IGNTTNTDSISIGLDTCDKFKRILCVQQ"
     gene            complement(21488..21664)
                     /locus_tag="PLF66_09075"
     CDS             complement(21488..21664)
                     /locus_tag="PLF66_09075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64231.1"
                     /translation="MFIESNVSPAYVSSYKNYPEVKKVFGKKILEILVCHQEGNRVLG
                     SQVPISILKKILLL"
     gene            21984..22136
                     /locus_tag="PLF66_09080"
     CDS             21984..22136
                     /locus_tag="PLF66_09080"
                     /inference="COORDINATES: protein motif:HMM:TIGR01552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HMZ64232.1"
                     /translation="MSTVLNIHEAKTNLSKLLSKVMQGEEVIISKQYILDTHTFLWAI
                     AEPESI"
     gene            22501..23385
                     /locus_tag="PLF66_09085"
     CDS             22501..23385
                     /locus_tag="PLF66_09085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64233.1"
                     /translation="MKKLSYLLVLLFTHCFTVYTQNESILESEKINSKTQIEKKDLYV
                     LTHTLTNDGLYLLLDAYQVEEENQVHFIKEKYEENKGVKLKTIFDDGIGGCDVQLCGA
                     ILVVTTTIFLLEIPTLPFRMMSEPKERIRKEIKTMKSNKIGVLSTENIYFSFLEKNSS
                     FKNYPFEEDKVLIPISELGLNSFNIKNFSYKLTFTSNGKKLREGNLDLSNDVKKSKEL
                     QAIVKENDSKNPAVQCKISFPILEKQDLEKNIAEKDCTETCKRKFSKPNVLVWNNDEY
                     NACLKSIENCYSITRIKK"
     gene            23530..24348
                     /locus_tag="PLF66_09090"
     CDS             23530..24348
                     /locus_tag="PLF66_09090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64234.1"
                     /translation="MKYCLLAIIFVQIQCASFKVYELAFPTSVNSASKSNIDSVKLLE
                     NNSIELKVKTLLSTSPKCIELIQTSQYYSVYSYSFKNCSLSDARKDLEQEKNTNPAIL
                     PILDQRKTDINLSEYDAYSKYSDYLPYVQEYLNEIQTKKVYINNKKDKIYLTSGGKYC
                     IILNRDARYPSVSNYKENPGNCPASYPQSDFKEINVFQAETAKLYLDFHDGRLVYTLP
                     KSNLRSIYLNIVELNRDDNYFYLLLMPTALVFDIVTFPFQLVYFWALGNAIPAH"
     gene            complement(24345..25097)
                     /locus_tag="PLF66_09095"
     CDS             complement(24345..25097)
                     /locus_tag="PLF66_09095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64235.1"
                     /translation="MKNLIRITVIILTGVYFTACIMTSGEIKGTIKNLEKSQFSAKGS
                     DLTLAICGWQPIAEMQFTKIDVDLFPDSSRSRGRGYLFIDGKGKGFLCSSKIFFTYTE
                     GYTGGHGTYESFLNFGVFERENPVISEVSQSEHSKKVQIGEEILGNINKSNTQIPDGS
                     YADFYKFENSLKTGIRIEIKKDSNEKNNSFDPRAFTYQNQKFLSKDDDTGTIVQKGLI
                     YIMVTSGDEVGRYRLKISELSEKEKSNLIGWP"
     gene            complement(25111..26217)
                     /locus_tag="PLF66_09100"
     CDS             complement(25111..26217)
                     /locus_tag="PLF66_09100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64236.1"
                     /translation="MKKTILVILSCLFLKNCLNKQLYEKTSLPRNKDFILVSGKIDPE
                     NYNDKMEVFIVTPENKKVELLDLLDETQISSLNYFKYREYLIFENGKKIKLRNKEGNL
                     DYPQNTILYQLPILNDKKDKILYSDLSSIKVFDLNGKKKKEIKIEHERAEWFKDDKVA
                     FLSDRKDKPGQSIQTIDLSSNKEETLYKSKQFISSFILSPDRTKLATIELDKVWNPIQ
                     YSVRIIDLTSGKILIQKNMPDYTKDIKWSTNGELGILFRMKAEEGLYPPFSIYLLSEK
                     TDKDLLQLKAYEPPGFIFSGEGYSGVEEFTWSPDGSKIAYIASEPGNCNRDEGGNVHC
                     DLDIYIVDINTGAKEKITNIKNKSFTFLFWKDRI"
     gene            complement(26214..27245)
                     /locus_tag="PLF66_09105"
     CDS             complement(26214..27245)
                     /locus_tag="PLF66_09105"
                     /inference="COORDINATES: protein motif:HMM:NF021466.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2183 domain-containing protein"
                     /protein_id="HMZ64237.1"
                     /translation="MLIRIVFFNSYTMFSSELKEDEDVILYPTYLYLDKSASEYKVKI
                     HLHVFKKKENSIKRKILIQELEKYLKVSDEDNSKIFQERIRSFLVDNKRGKKVSVRVL
                     GQDYKLKETEANGRSITEIKIPKKSILENTNQIEVNVNSSSRNKKEYKGKIWIIPEDS
                     TCLISDVDDTIKLSDVRNKKKLLENSFIKPFEKISGMNDYYISLTSNVSCFLFVSASP
                     WQMYNHLSKFFLDEKFPESIYFMKDIRLKDWSVLNLFESPDNYKIQKIEPILKEWKNV
                     KFILVGDSGEKDPEAYATLFRKYPDQIKKIYIRVAYDENLEERIKRVFSGIEKDKYFF
                     FKDPREIKE"
     gene            complement(27268..30060)
                     /locus_tag="PLF66_09110"
     CDS             complement(27268..30060)
                     /locus_tag="PLF66_09110"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HMZ64238.1"
                     /translation="MSFRIVIIFVSLLSFSLYSQTKEGSLSKVVVLGDFNPYKSEKNP
                     AIENQIKESMKSNLESNKFQVTVASGSLQDNMNYAKQKDAAFLIEGFYTSGTETKNLS
                     VFIQIYNPNTGYMIDAISIVDELDIVDGVQLDAKELKEKDETIIQKIANKTSNSIRSN
                     NNRKENRENINQNAIANGITEKNKLPVRENSKADEEASAEVFNLLQNQVTTSATKIAR
                     KTSEAPNIVSVISHKEIQDYGRVSINDILYQLPGFSPSQDYDRRTVSSRGVFEGWNSN
                     HLLMLVDGIQFNDNLYGSAYTSEITPINMIKSLEVIRGPGSALYGSNATNGVVSINTF
                     SGSDLGGSLKTRIRGGNFGTRIYDIITGNKGNLFSYVVSYNSYQTDGNNVKNYDGSGR
                     TDPVFTNYLQKFTTNDTRNSNYLFTKLEGEGALKGLTIQYHRQYWSFQTGQGWLWRTP
                     DVKEAMAETRQIVTMKYTKNITEKLIQEYAIRYQNRSIDWNTRYAENGAYDGYYPGGV
                     TEYLKTGANDIFGRGQLTYIFPNGGTALGGVEANRFVYTGDSEHYSNINLSDSVNAFP
                     PYASGGYVKQGPWLEWIKNKPIPKMAAFGQITSGKILNRSLEITFGIRYDETQAKFRG
                     VDQPRSGLLGTPSLDGTPIPSQYLGDPFVPNEKRIFKRTSPRVGFVYFVSNNLTLKLM
                     SGKAFREPSITELFGFNTFSLASNPRKLKPEVIHTTEVGVDWLINKYINIRGNLFRTR
                     YENQIAYSVANNNLSTNLYTLTTGGAEGEALFTYKNLSGFLNFSYAKRLNEFIQDRTI
                     SIDKNQITWVPSHTANLGITWTDQKFMTSISFERQGSVYRRNSDLGPVDPIFGFNTNL
                     PPYEYNYPVYRPKVVPAWINVNYRFMYKWNESVEMGINVMNLFNSHQTLLKNNNFPFD
                     YIREGRRFLFDVTASF"
     gene            complement(30546..31802)
                     /locus_tag="PLF66_09115"
     CDS             complement(30546..31802)
                     /locus_tag="PLF66_09115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HMZ64239.1"
                     /translation="MQITSKIQKKVLVLILGGGKGTRLLPLTEKRSKPAVSFGGKYRL
                     VDIPISNALNSGFEQIFVLTQFNSYSLNRHISRTYNFQSIHKQGFVEIIAAEQTVSSV
                     RWFEGTADAVRKVLPHIEDYKSDYIIILSGDQLYNMDLNQFLITHLSDPSNQMTIAAT
                     PVTEDLAPALGILRKDEDNWTTGFYEKPQDIGEVESFRLPNRTFLASMGIYLFNTSTL
                     KELLADTSLTDFGKEIIPGALKTHKVKTYVYDGYWEDIGTIRAFYEANLMLTDDFPKF
                     NLYLESTPFYTRARSLPPTKVNGAHLNKVLLSEGCIINESNITRSILGVRQVVDSGTH
                     IENSVVMGADSYGSFDKKGFGIPVGIGKDCEIKHAIIDKDCYIGNNVRLINKENHENY
                     EDDFVKIIDGIIVVPRRSAIPNGYEI"
     gene            complement(32008..32670)
                     /gene="purQ"
                     /locus_tag="PLF66_09120"
     CDS             complement(32008..32670)
                     /gene="purQ"
                     /locus_tag="PLF66_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004462682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HMZ64240.1"
                     /translation="MKTAVIRFPGSNCDRDIADILSKYYHHDVTYVWHKDSFEKKYDL
                     VVLPGGFSYGDYLRCGALARFSPAMISLKDHLDSGRLVLGICNGFQILVEAGLLPGAL
                     TRNKTLKHICKNVNLKVGSKENPLTKKISPDKLLDIPISHGEGSYFADKETLKRLEDE
                     NRILFKYSGENPNGSLEDIAGITSKDFKIAGLMPHPERAVEEISGSTDGKIIFDSFFA
                     LI"
     gene            complement(32667..32915)
                     /gene="purS"
                     /locus_tag="PLF66_09125"
     CDS             complement(32667..32915)
                     /gene="purS"
                     /locus_tag="PLF66_09125"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008593368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HMZ64241.1"
                     /translation="MFIGKVNITLKESVLDPQGTTVKKVLHDMGEEMVSDLRIGKYIE
                     IKLNSKNIETAREDADRLCKKLLVNSVIETYKLEVIPE"
     gene            complement(32897..33748)
                     /locus_tag="PLF66_09130"
     CDS             complement(32897..33748)
                     /locus_tag="PLF66_09130"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016752699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HMZ64242.1"
                     /translation="MKVAYKGKVRDVYDLGDYLILSSTDRISAFDCVFNEIVPDKGKI
                     LNNISTLWFKYFSDIPNHIVETDYNQFPGEYKGREELKDRSVLVKKCKRIDYECVVRG
                     YLSGSAYKEYKSTGQIAGVDVSRKYQESEELSEPLFTPAIKNDTGHDENISEKDLKDK
                     IGLELFTILKDYSMKLYLGGRERVKTEGLILCDTKFEFGFYNDKVILIDELLTPDSSR
                     YWELNTYAIGSSPPSYDKQILRNYLEKIEWNKNPPPPKLPDSIINELILKYKELQGKI
                     KRCLSEK"
     gene            complement(33754..34452)
                     /gene="ccsA"
                     /locus_tag="PLF66_09135"
     CDS             complement(33754..34452)
                     /gene="ccsA"
                     /locus_tag="PLF66_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002974119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HMZ64243.1"
                     /translation="MNEKIRIFSPMLDIVLFVLFVLFFPFAVILSLYYPNVILEQGLS
                     HRIFYFHVSVAWVALYAPALSALFGLIYIVRKNPDWDTLSYSLNKLSLLFSILVLISG
                     PIWAYSAWGTPWDWTDARLQSFFILVVSLLSYFVFRSLITDRNKKYLYSSFLSLLCAL
                     NALLTWGAIRWIENPGNHPGSVLGRGGMDKDMKETFWVNVLAYHILFLILFLIIYRKE
                     KLDSKLEQVRGEME"
     gene            complement(34480..35139)
                     /locus_tag="PLF66_09140"
     CDS             complement(34480..35139)
                     /locus_tag="PLF66_09140"
                     /inference="COORDINATES: protein motif:HMM:NF015344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HMZ64244.1"
                     /translation="MFRHLLWKEFLLIGRAKNGLLSLVSLLMAFLFIFHYSLETNTKL
                     EIDSIIGLKWAGIFLLAFVLISQVTYEERESGAYRITRLYISSHSEFIAKSFVLFFLL
                     FLVELFLLLLLFLFFEKFYFESSKLIGQFLYLIPGTLSLSFLGVTLSQLSFATRLKEI
                     VLPILLIPLSIPVLLSGMQAERKYFSSAEINFPSLIVLFAFMILYLALGLLIREVGED
                     I"
     gene            complement(35132..35758)
                     /locus_tag="PLF66_09145"
     CDS             complement(35132..35758)
                     /locus_tag="PLF66_09145"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HMZ64245.1"
                     /translation="MKTSILSCKNLSYTIGYKKIFKSISFEIFASEFTLLTGENGSGK
                     SSLLKLILQSSKRKEFQWNDNFNSKNINPISYLGHENGLYSSLTLRENLNFFNRILKN
                     PMPESDLNILLERLNLIKRLDDPISTFSEGMKKKSGIIRALLARPLLLLLDEPLNGLD
                     VNSAEAFLNILKTDFKDSAILLVSHEIDKLSKLVKNRLHIYNGEINYV"
     gene            35790..36839
                     /locus_tag="PLF66_09150"
     CDS             35790..36839
                     /locus_tag="PLF66_09150"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HMZ64246.1"
                     /translation="MLYFQLLFLLFVFQIFPQTIVIDDEKFQMNLWDQELDQKQKVDF
                     TKGSFLKNENDFILCKGRMWSGNPPKSTYLFTYREKQIDNIGQFNNEAIELIERNEEG
                     DWSEANAMLSAMIQADPMFFPARYNYARFLFIQKKFQEASYEFLQARNIIPAYYRSYL
                     HLGHIYLFLGEKQKAEEIYKEAAWRNPLNDEAHISLAEIYWKEGLITRARDRIKEADK
                     TNDPTEATIIRRQKPAFPVLDFTKAIENPLANPNPKIAEALLYFHQEKYALAYKLFKS
                     INTEHLNKEKIPYLKKMHYYFAETAIKLLDYKTATSQYDAMLKFPFDPFFQEVSMATV
                     ERKKNLTASIKQNSD"
     gene            36977..39451
                     /locus_tag="PLF66_09155"
     CDS             36977..39451
                     /locus_tag="PLF66_09155"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HMZ64247.1"
                     /translation="MLFIQFPIYSLEKINLAEDCERNGSTESCHSHPWYVINQFSEEF
                     INSEKISGNWKLADKFPLTPESYYPDTADTLFTMRTQFDAPTEYLTNLSFPALYFISL
                     GEVYSIYLNGTLVYQDGKSENGKILISRYGKNILVPLSSKLFKEKGNLLVIKVEGTRG
                     FGATGVYTGNSVYITDYRDAAAVARDRIGLILIFLYLFVGAYHLFLYFKRREEVYNLY
                     FTLLSITTFLYMLNRIGFFHEQGFDSVLGYRMELLSAYGIFPFIVAFMESLFFGKLSK
                     LLKGISLSYAIIGIATLFSPPPILNQLLLLWQLSVLLGIVYTFYIFYKAIKEKSDNAK
                     RLFFGFLILIVFSVFDILDSIIFKTGFHFARYAFFAFVTGIAVILANRFLNLYETIED
                     LNQSLEKKVELRTKELRYTLDQVRLLKEQQDGDYFLTTLIIQPLATNTASDDRLELDI
                     LMRQKKKFEFKKRVYEIGGDICISDIITLKEKKYTVFINGDAMGKSIQGAGGAIVLGV
                     VFKSIISRTVMYKGNQNVYPERWLKIAFIELQKIFESFDGSMLVSIVIGLMDNESGFL
                     YFINAEHPWSVLYRDEKASFLESGFHLRKVGMLGVEGSLKVKTFKMHKNDIIFIGSDG
                     RDDIQLGEDETGRRIINENENLFLQTIEEAHGDLETILSITEKKGEITDDFSLLKIKY
                     KGDEPIYLSDRPIFIENAISAIQKDKPIEALQILEQNKNEELGQYAKDYAQLVLKAAT
                     LSTRLQAGSTLLNEYIWKFPEDESAIYGLATLYFKQGQFEKATDLGEILKLRNPDDSK
                     VLKLLVEIYSASGNHTRAEKLKLEVR"
     gene            complement(39572..40933)
                     /locus_tag="PLF66_09160"
     CDS             complement(39572..40933)
                     /locus_tag="PLF66_09160"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF012457.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008422 - beta-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0030245 - cellulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH1 family beta-glucosidase"
                     /protein_id="HMZ64248.1"
                     /translation="MQKILFPKDFIWGTATAAYQIEGAYNQDGKGLSVWDDFTHKKGK
                     IKNGENGDITCDHYNRYKEDIALMSKLGYKNYRFSIAWTRVLPEGKGQVNSKGLDFYD
                     RLVDELLKKKINPFITLFHWDLPLALEKEGGWYNRNTAEYFGEFTEVILKKLGDRVTN
                     WITLNEPWIVFVCGYLLGVFPPGVFRPFSAFKVAHNLLLAHGTALARIKSIYPKSKVG
                     LTNALSPVHNFLLTTEPKSTRRADAIVNRLWLDPIYKGKYPEEILPFIEKQNKGNILP
                     DDLKLISGKTDFLGVNNYTRFVMRYFPFPIYNFRPAKPFYKGIQFTSMGWEIYPQGLY
                     ELMKMIREDYGNPPVLITENGVAFIEKPNEKGIIEDKNRISFLQQYLSSLHKAILEGS
                     NVKGYFVWSFMDNFEWAEGFEKTFGLVHIDRNTLKRTPKLSANWYSKVCKENGFSYDL
                     NLG"
     gene            complement(41421..42671)
                     /gene="ccrA"
                     /locus_tag="PLF66_09165"
     CDS             complement(41421..42671)
                     /gene="ccrA"
                     /locus_tag="PLF66_09165"
                     /EC_number="1.3.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004444708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043880 - crotonyl-CoA reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonyl-CoA carboxylase/reductase"
                     /protein_id="HMZ64249.1"
                     /translation="MSETPEIVPVGTLPPVGVVPKKMHAQVIRPERFGDPEKAFQLEQ
                     IDVPEIKPDEVLVAVMAAGVNYNNVWAALGYPVDVIGARNKKGEPEKFHIGGSDASGI
                     VYKIGSEVKNVKVGDEVVVHCGVWDKNDPFVKAGNDPMFAPSQLIWGYETNWGSFAQF
                     CKVQDHQCLPKPKHLSWEESAAYMLVGATAYRMLHNWKPNDVKPGDPVLIWGGAGGLG
                     SMAIQIVKAAGGRPIAVVSSDDKIDYCKKLGAEGVINRNNFKHWGAITSDINKPEVFA
                     EWTKKARDFGKALWDIWGKGVNPKIVFEHPGESTIPTSAFVCETGGMIVICAGTSGFN
                     ATVDLRYLWMRQKRLQGSHFANDENSKGFNDLVIAGKIDPCLSRTFKFEETGSCHQLM
                     KNNKHPHGNMSILVGAPSLGLGKK"
     gene            complement(42961..43989)
                     /locus_tag="PLF66_09170"
     CDS             complement(42961..43989)
                     /locus_tag="PLF66_09170"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HMZ64250.1"
                     /translation="MKYSQLGNSGLVVSKLSIGTMLFGGEGDYFGLKYSIDQKTADAL
                     ISKSIEMGINLFDTANMYNNGQSEIMLGKALGSRRKSVLISSKLAFRTGEEVFNAGIS
                     YKQIIEQCEASLKRLNTDYLDILFLHTEDPITPLEEIAKALENLQMQGKIRYAGVSNW
                     QAWKTASMVQLQKERRYSPLVSAQMHYSLLNRSIEEEFIPMSSHHGLGLMIWSPLSSG
                     FLTGKYTRSNPKPENGRLNTFDLNLFDREKAYTVVEKVSELAKKQNTTSTAISIAWLL
                     AKKICATILVGVSKLEQLQDNIDSLALDLSTEEIALLDEVSKQDVRYPSNFLNFLDSK
                     LKEAKVFS"
     gene            complement(44009..44497)
                     /locus_tag="PLF66_09175"
     CDS             complement(44009..44497)
                     /locus_tag="PLF66_09175"
                     /inference="COORDINATES: protein motif:HMM:NF024966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HMZ64251.1"
                     /translation="MKKISKIILTLILVITLLQNCMNRDLQELNAKQDVIEKVNELFI
                     FTDNRDWEKVKSVFADRVDFDMTSLAGGKPNLLANTEITSAWENGLKNLKAIHHQAGN
                     YLVQINGDTADVFCYATATHYLPVKDGKNVKTFVGSYDIHLVKKEKWYIDRFKFNVKY
                     VE"
     gene            complement(44603..46528)
                     /locus_tag="PLF66_09180"
     CDS             complement(44603..46528)
                     /locus_tag="PLF66_09180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64252.1"
                     /translation="MILSLILSINLYSHDYRDDSKKIYKKPIEVITKERREELEKYIA
                     DNAEKPSYQISKIKSLLNEYYAQFVEEKRILDVREKAGRYKDRKDNVTDEFKNNSGVI
                     PVNADVNSIRLFISNQKFKNAPSQLARDSDLLYDLHLRLAKLYEKIGDRNNTIESYYS
                     ALRYRDYSNTEERYYNPSLLEELSEEEAAQRLAHKKIKDDLEEAKQNLKKAEDNSARI
                     GSLYAKRDLSEAEANVQLSENRSKIDSLRDEVKAKEKEYSDSLLGRFGKYVNDKTSSD
                     SKTIVDLAKQIQELENETKEKSKVDEKMNHFGRGNFVASDTNRNQDYVGYSQLLEFAI
                     KVNPNNAEAILLLADEYKSSAKKRNALDFYLRYIKLSSLPENNGKYPLYDVYRSVAAL
                     YTDLKQYVLASNYYEKMLEILNSDKKEDPNFFYQLGDFYSHRLGNFEKGSDYFSKWLT
                     WLDQKRKEEKEKRESDKEFGISLEETTRRLSSEFIASYAISSYKKQMQYPEEEQIYLE
                     RAYSAYKEISTLLKDQDASLANVKRDVDLLKRDLLNKSTAESLSMYKEEQEKLEEASF
                     RLKSIKIVYDSLKKTNLLFRMTALEEENRNFKRVKNLYEEIVNIGNENEIANALRNIE
                     RIKKIEADGISRKRIVH"
     gene            complement(46606..47070)
                     /locus_tag="PLF66_09185"
     CDS             complement(46606..47070)
                     /locus_tag="PLF66_09185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004754541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HMZ64253.1"
                     /translation="MAKDDDHLSEQYITFTIGEEDYAISIINVEEIVKVTNLIKVPKS
                     QNYFVGLMDIRGKVVNMIDLSKKIMNKKVGESSMNRAIIVKIDGKSLGVIVDKVSHVV
                     HFSPAQIDPPPPSVRGMSSRYIIGVAKKDNRFIIIMDIEKILSSEELAEVTK"
     gene            complement(47081..47797)
                     /locus_tag="PLF66_09190"
     CDS             complement(47081..47797)
                     /locus_tag="PLF66_09190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64254.1"
                     /translation="MLYDSDYEGFRTWWKRSKTPARGYFKSYRAVSPIGDVFVVDYNF
                     HENLVRLSLEPASEKGRTYTATIKNGTIIRERDVFNATNTSLKKKFHPFKNVFSCIPD
                     DDVLNSIGGVYDISKVSLGKKAHEEIPSDAGELEPYTQVSGESFYKRLLHRAKDSYAD
                     FKIRFLDDLYDSILGVSVCLAIYLHFFDYVVLGFSLAFMGVFFGGLDWILRNRTPFYL
                     KVMSFLSFGGYYFYTGFTRF"
     gene            complement(48022..>49011)
                     /locus_tag="PLF66_09195"
     CDS             complement(48022..>49011)
                     /locus_tag="PLF66_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001070779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HMZ64255.1"
                     /translation="VSTPKQLQKVYSNKTLLRETIDRALTLTTINRIFIGTNAALKKS
                     ILQQEKGFPEKNFILEPEGKNTAPIVALASLYFKQKFGNPTQIVLSADAFIEPVNEFT
                     KTLKLAMQEAEESLVLLGIKPVRPETGYGYIGTGKPTKYGMQVKGFFEKPDYKVALKY
                     LKKKNFYWNPGIFVWKTDFILSEFEKHAPYILSPLKNKFPFANFGELSAAFKLLPSEA
                     IDTAIMEKSSMIRMVKASFDWDDVGSWLSLERVLKGDSQGNHHTGKASFHFHAKGNIS
                     STKKELVTFLGVEDLIVVEEDDVLFISSKTGIKDIKLLLSELKKNRSLQKYLE"
BASE COUNT        16415 a         8653 c         8119 g        15824 t
ORIGIN      
        1 tgaccacttc aattctattt tacaaacgtg cgcgacttga caaagtcgca ctaataaaat
       61 tttataatta ttaattattg aatttgctgc gcaataaata tacatattcg tggctgtctg
      121 ggaatcaagt ataaagcttt gaatggtttc gtttacattc aatacaaaat taaagctaga
      181 atcaatggta gaactattag tggttggagt tattgctttt gtataaactg agcaattttt
      241 cgtaccgata gccgaaatat taaataccct ttttgaaaaa tccatctggc taaaactaaa
      301 aggataaata tcaacatcgg ttgagctgat ttgaccaaaa actcctaagg attgagacgc
      361 taagggcaag gttaatacaa tagatgaatc gctttggata aaactatcat ttggttcttt
      421 ctcttgtttg agtatagatt taaatgttag aaaataattt tccgttgcac ttaccggtgc
      481 aggattaaca atatcattga aaaccagaaa cccaattatt tctccctctt tacatccttg
      541 taaacattta tcataatctt gcttgttcca aaaataacga ttctcttgga aacatcctaa
      601 tagcaataga gaaataatta ttaaactttt aatcatctca accttctttt tagtaattca
      661 ttcaattatt taatactatt gaaagagctt ttttattttc aataagatat agcccaaaat
      721 tgaactatga tactcttctt attttgtatc aagttagaat aaaattgccc aaattgattt
      781 cgggcaaatt actaaaatca ggactaatac aatttataag aattttttta taagtattct
      841 cttctattaa tttagagatg ataaatactc gtcaacctat ttgattttat aactgcattc
      901 tttttctaat ctactttata agctgatttt aaaaaatgga tttaatttat gaggtaggca
      961 atgtcaattt tcactacaat caacaactac gggaaaatgg taaaattttc tcatacactc
     1021 ttcgctttac cttttgcagg acttgcgagt gtaatcgcat tattaaaatc aaatctaagc
     1081 acggaagact taatctggaa actaatactc atcctacttt gtatgtttac agcaagaagt
     1141 gcggcaatgg gttttaaccg ttatgcggat agagaattcg ataaagcaaa tccaagaaca
     1201 gcaaatagag aaattccatc aggagtctta tctgaaaaat cagttctttc ttttacgatt
     1261 ctatttggga ttagtttttt aatttttagt tatctactta gtccgctttg ttttatctta
     1321 tccattccgg cacttgcaat tattttattt tatagttaca gcaagcggtt tacaatgttt
     1381 tgccacttca tcttaggtct tggaattgga atttctccaa gtggtgcttg ggttgcaatt
     1441 caaaataatt tagaactcat tccagctctt tggtcttttg ggctcatgtt tcatatcgct
     1501 ggttttgata ttttgtattc ctcgcaagat gtggaagtag ataaaaaact aggactctac
     1561 tctattccaa gtcgtcttgg tattccaact gcttttttaa tcgcaaagat ttcacatgta
     1621 attgcatttg ttttgtttgt atatgcagga tacttagcgc aattagaatt tttctattat
     1681 ctatttcttg gaatcactgg aattctattt atagtggagc atacacttgt tagccccact
     1741 gatctaaaaa aaattccaat tgcatttttt catatcaatg cttcgattag tgtgattcta
     1801 tttattggaa ttcttctaga cagaggaaaa gacttatata caaaatttgt gcaatagtaa
     1861 aaaaattgat ttaggaaaaa aaatgaatca aaataaatct ctatctattt ataggtctat
     1921 ggcaaaacga ttatgaaatt agttttagga attgcgggag caagtggctc catctaccca
     1981 gctcgtttta ttaaaaaatt attagaaata aaaggggaaa cctttttaat tataagtcca
     2041 gcttccattc gaatctttcg agaggaatat gaaaccaaag tcaataccgc acaagagatt
     2101 cttgatttta ttataaatcg ctgggaaatc aaaaacattc aaaaccaaat tcactttcgt
     2161 tctttccaag acattggagc agatattgca tctggttcta atacttggga tgggatggta
     2221 gttcttccct gttctatgaa gactatcgca tcaatcaatg caggactcac tgaaaattta
     2281 attgaaagat gtgccgatgt aactctaaaa gaaagaaggc ggctaatcct tgttccccgc
     2341 gagactccct acaatcaaat tcatttgcga aatatgcttt ccctcgatca agcaggtgca
     2401 atcatccttc ccgcttctcc cggcttctat caaatgccaa agacactcga tgacttggga
     2461 gattttatcg caggaagaat tttcaattta ctcggagtca atgcagatct cttccctaaa
     2521 tgggaaggat aaaaagactt gcaattcttt ttggttttgc ttaacctgaa cttgatataa
     2581 gaatttttaa ccacggatgg acacagatgg acacagatgg ttttaaattt gttgtatcaa
     2641 actgacgtta gttacaattt atgagtagta tttaacggct taagaaagca aacatcattt
     2701 gaacggttgg ttcgccttat ataaggtgag ttgctaggag gaaaaaatga atacctttgt
     2761 tttagagata cccgattcca ttggcaagat tgatgatgaa aagtatttag atctctgcaa
     2821 aaaaaatcct gaatcgagga ttgagagaag tagcaaagga gaaattatta ttatggcacc
     2881 aaccggcggg gaaacaggta gaagaaattt cgaattagga atacgttttg gagaatggaa
     2941 cagaaaaaag aaattaggta agttttttga ctcctccact gcattcaaat tgcctaaagg
     3001 cggaattcgt tctcctgatg ttggatttgt gtctaacgct aaatggaatt ccatttccct
     3061 agaagataaa aagaaatttc ctcctctctg tcctgaattt gtattagagc ttctttctga
     3121 ttctgattcc attgaaacct ctcaaaagaa aatggaagaa tggatggaca atggatgtct
     3181 tttgtcatgg ctcattgatc tagatgaaaa gaaaatctac atctatcgca aaaacaaacc
     3241 agtagaaacg ttactcggtc tagaccacat cctctccggc gaagatgttc tccctgaatt
     3301 tacgctcgat ttaaaagaga tagagtagaa cttaaaacag cctttcattc tggaatcata
     3361 agagagtggg aaaatgtgat gtattggacg tcagaagaat tttcttctga tagcgcatat
     3421 tactttgatt cctctatgga ttcgaaagcg atatcccgaa aaacgatgtt atctttgtcc
     3481 gctgcattcg atgatttgcg gttactcaat attaacccca tccattaaat aattgctaag
     3541 acttttttat ttttaacaat ttgaatttca gcacctagat aatttaaaat tttctttaga
     3601 gtatcaattc taggattttc ttttccattt ctaattttca aaatcgttct agttgaaatg
     3661 tttagagctt tagaaagttc ccgaatggat gcttcatcat cttgcatgag agagtaaacc
     3721 aattcaaaaa ccaagaagtc tttataaccc tcatcaaata cttttttctt ttttagatct
     3781 tttaattcct gattcaaaac acttttaaac gaaattgttt tttctttact cttcataaaa
     3841 ttccttcctc gcaacctgtg ctttttttaa ttctaatgca ggtagttttt gttccttctt
     3901 tataaatcca tgtgttataa taattttctc tttttccata aatgcaataa atcttatagt
     3961 ttaacgcacc ttctgcaaga tgaaccaacc atttcaaatg aggtctgctc ctagcggagc
     4021 gctttcctcc catcgctttt tcgctctcct tcagccgatc tataccgcag gcgattaatt
     4081 cgttcaacag caatatctaa aattatcaaa atatttattt ctaaattacc aaaattacaa
     4141 aaacataaat attaaacaat aatcaatctg cttctgtatt gaaaatttat ttttaattct
     4201 ttggatttaa tttagacaaa tattcttatt ctatctttag tgtccttatg aagaagataa
     4261 ttatagcaat aataatgata atagcaacat cgatatttag ccagaactct gaaaaatgga
     4321 gtgattatca aggtgtaatg gattgggaaa cggcaaacaa aaaatgttca agtattaata
     4381 tgaggttacc atctattgaa gaattgaaag tagctatgaa agaaggttcc ggtaaacaat
     4441 ggaaaaaaga tggattatct tattggtcaa atgctcctag tggcggatac ggtggtgcat
     4501 acagtattac tatcgaggct gctggatata gaataagtaa tcagaaggat ggatatctaa
     4561 atgttcgttg tattgacggg ctttcgatta tgaataaaat cgtgaaagaa aaaatttcaa
     4621 tgcaatggag tgaactacag ggatcaggca attgggaata tgcaaacatt aaatgcacaa
     4681 atataaaaat gaaattacct agcatagatg aacttaaata ttcaattaag gatgatgtaa
     4741 ttaaatcatg ggaaaaagat ggactttatt actggtcatc tgagccgttt cattatattg
     4801 atattaagaa tggaagtatt aaagaaaata tgactgttga tgataaattt aacgttagat
     4861 gcacgcatgg acttccaata tcaaaaaaat ggattggtgg ataccaaggt gatatgagtt
     4921 ggcaagaagc gactgaaaga tgtgcaaatc taggcttgag attacctact ataaatgaat
     4981 taaaggcagc gtatttatca aagactatga aatcatggga ttacaattat ataactaggc
     5041 atttttggtc atccactccg agtccaaatt catcacatta tacattttct aacggtgaca
     5101 aaacagacct ttcgcctagt gatgcatctg tgtattgcat acaatgatta cttttttaaa
     5161 gaaaattaga taatttatgg cgtctatcag ataatagatt tatttagaga ttcatgctga
     5221 atagataatc ctacaatttt tatatatatc caacccttcg tgcttattcc acccttcgct
     5281 ccaagctttc gcgaccaacg ccttcgcatg tgttcatccg tggttaaatc ttttatttca
     5341 agaaatagaa aagacaaatc gcattgcaca aaacgtcagg tcgcgtgacc tgacctacaa
     5401 tttttttaat tcatcccaac ccttcgtgcc tcttcgtgct cttcgtggtg aagaaaacaa
     5461 cacatcggtg cctatcggtg ttcatcggtg gtaatctttt ctccctctaa ctttttttct
     5521 tatcctcttc cgtgattcga tctaggctta gtttacgcac acgatcatcc atgacatgga
     5581 gcaaacgcag gtttacctct ttcatcattt gaccaactac ttgagtgctt aacgtttcta
     5641 aaatagcttc tgtgacttcg tcacttactt tgcttgtaac ttcttgcata aaaggaagag
     5701 cattcacacg tttcatgaaa tttttagatt ctaaggtttc tcgcacaact tttcccaaat
     5761 catttttatt ctttgcaatg atgtcttttg ttagatcttg aaaatccatt ttttcaacta
     5821 cttcttctac tctttcgaga gatcgtaaga atatcgcatc tgaaattgca tctacaaaca
     5881 aatcaataaa acggaaattc aattctcttt tgataaattg ttttgtatcc ttctctcctc
     5941 ttttgataaa cttataggta ataatcatga gctttagagt cgcaagagcc aaaaagaaac
     6001 gaaagatagg aaagggaaca agtcctggga gtagatacca atttgtgctg atatattcaa
     6061 acttatcagg agaacgtcca attctaaaaa taacttgaac accccagaga gtcacaacaa
     6121 acatatcaaa taaaaaagca gcaccagaat aaatctttgg taaaaatgcc tcataggaat
     6181 ttagaaatag aataaatatt aaatcaaaaa ataggatgat atgtagaaaa tagtcctttt
     6241 ttgaggtagg aatataaggt ccccaaggat gtaaatattc aatgagttta cgaatcgaaa
     6301 atttcataga agagcagatt aaagcatact gctttttttt ctactaaata aaaaccaata
     6361 ccgatataga agattaccac cgatgaagac gatgaacacc gataaaagat tcgataattg
     6421 aaaatttaca tcgtttttat cgttttgtta tcggtgccat cggtggtaat ccgtctttct
     6481 ccatggtaaa aaaaacttac gttaatgaca ttgacaagtt ttacgaccta aactataggt
     6541 gtaacttacc agagaggaat atcatttgtc taagagttta tacataacca caacagaacc
     6601 aaagagcggc aaatccctag tatccttagg gatcatggaa ttattaaaaa gtggactctc
     6661 tcgagtcgcc ttttttagac caattataaa aaaccgtgat catggaaaga tggatccaga
     6721 tatagatcta atccgaaagc actacaattt acaaatcaaa tacgaagata cctatggttt
     6781 atttatggac caagcgttag agctgattgc tgccgacaaa aaaagcgcgc tcattgatac
     6841 gattatggca aaatgtaaaa agctagaaga agaatacgat tttgtcctaa ttgaaggctc
     6901 tgatttttct gggtcgacta gttcttttga attcgaactc aattcagaac tggctaaaaa
     6961 cctaggggct cctattatgc tcgttcttcc agctacccat aaagacaacg acagtgttct
     7021 ttccgatttg tctcttgcaa ttgaatcgta tacaaacagt ggctgcacga tcataggatc
     7081 tattcttaca agagtgaatc ctgaaaatct aactcaacta aaaaaatctt atgaatttaa
     7141 gacgatagac aacataccag cctacttcat tcccgaaaac gaaatcattc gaactcttac
     7201 catgaaagaa atcacagaag cgcttgaggc agaggttttg tatggaagtg aacacctaat
     7261 gcgtcacgct tactataata cagttgcatc aatgcgactc gaaaattttc taaatcatat
     7321 taaagatgga accttagtca tagtttctgg agatagagcg gatattatac tcgggggatt
     7381 tttatgttcc gaaaataaaa acttccctag aatcatggga ctagttttga caggaggaat
     7441 tcatcccgat cacaacattc gcaaaatcat agatggtcta gacgctcata ttccagtcat
     7501 tgtaacaaaa gaagacacac tccgaactgt ttataaaatc aatagcattg attccaaagt
     7561 atccccagac gatagacgaa aaatttctat ctacctaggt ctattcgaat caaatgtaga
     7621 aactaaaaaa ttaaaagaga agatgattgt ttacaaatcg agtgtaatca ctcctaagat
     7681 gtttgaatat gatctcattc aaaaagcaaa gtctttgaaa aaacatatcg ttttaccaga
     7741 aggaaatgaa gaaagaattc tgagagccgc cgaagtatta gtagatagag atgttgtaaa
     7801 ggtaactcta ttaggcaaag aaaaagaaat tcgggaaaaa ataaaaaagc taggtctcaa
     7861 actaaaagat gtaaatattc tagagccagc ccactctgct gaatttcaaa attactatga
     7921 aacttattat gaactgagaa agcacaaggg aattacaatc gacaatgcaa aagatgtaat
     7981 gagtgacgtt agttactacg gaaccatgat ggtgtataaa ggtcatgcag atggaatggt
     8041 ttctggatcg gtaaatacaa cccaacatac aatccgacct tctttagaat ttgtaaaaac
     8101 aaaaaaaggt ttctccattg tatcgagtgt attttttatg tgtttgccaa acaaagtttt
     8161 ggtttacgga gattgtgcag taaatccaaa tccaaccgca caggaattag ctgaaatcgc
     8221 aatgtcctca gcggaaacgg caaagcaatt tggaattgaa cctagaattg caatgctttc
     8281 ttattctaca ggagattctg gaaaaggaga agaggtagaa aaagtaagag aagcaaccca
     8341 aattgtaaaa gcaaaagatc caaatctttt aatcgaaggt ccgattcaat acgatgctgc
     8401 gatagatcca gatgttgctt ctactaaaat gccaaatagt ccggttgcag gaaaagcaac
     8461 tgtatttatt tttccggatt taaacacagg gaataatacc tacaaagcag tccagagatc
     8521 ggctaacgct gttgcaatcg gtcctgtttt acagggaatt aaaaaaccag taaatgattt
     8581 gagtagaggt tgcacggttc cagacatcat taacacggtt gtaattacag caattcaagc
     8641 acaggagtaa aaatgaaaat acttgtaatc aatactggaa gttcttctat aaaataccaa
     8701 ctctttcgga tggatgacaa aacagtatta gcctcgggaa tcatcgaaag aattggagaa
     8761 gaaaaatcta aacacaaata cagttcaatc aacttaaaag aaagtattga aatagaaaag
     8821 aagattgccg atcaccaaac tggaatcaaa gaaatggatg aactacttac tgacgcaaaa
     8881 acaggagtga ttcacaattc aaaggaaatt acggctatcg gtcatagagt ggtgcatgga
     8941 ggagaaattt ttacaaaacc aatcatcatt gatccagatg tgattcgagc gattgaggaa
     9001 aatattccgc tcgccccact ccacaaccct ggaaatcttt ctggaatttt tgcggcacaa
     9061 acttttttta aaggtgtgcc taacgtcgca gtctttgata cagcatttca tcaaacaatg
     9121 ccggaaaaaa actttcgcta tgcgcttcca aatatatttt atgaaaagca taaaattcgt
     9181 aggtatggat ttcatggaac atctcataga tatgtttcaa agaccgttgc atcttttcta
     9241 aataaaaaat tggaagaggt aaacttaatc acattgcatc tgggaaatgg agcgagtgta
     9301 tgttctgttc gagagggaaa aagttttgat acctcaatgg gtatgactcc actcgaaggt
     9361 cttattatgg gaacccgcac gggtgacatt gatccagcga ttccttttta tatggcagat
     9421 catatcaaac tttcctacaa agaaattgat aaaatcttaa acaaggaaag cggcttaaaa
     9481 ggaatctctg gactaaacga tatgagagat atactagaaa aagaaaagac aggcgataaa
     9541 aatgcgagac ttgctcttga aatgtatgta aatcgaatcc gcaaatacat cggctcttac
     9601 ttaattggtc tagatggaaa cttagatgca attgtattca cagctggaat tggggaaaat
     9661 tctcccgaca taagagagcg tgtcgtaaaa aacctaactt ctctcggaat cgaaatcgac
     9721 gatacaaaaa acaaaacgag aagcaaagaa ataagagaaa tcaattctga taaaagcaaa
     9781 atcaaaattc tagtttgtcc aaccaacgaa gaattagaaa ttgcagaaca agtaaaagaa
     9841 gtaattgaaa tgtgatcttg ttttgataaa aatagggctt agctaatgat ctcgatctaa
     9901 ggtgactctc aaagattacc accgatgggg acgatgaaca ccgataaaaa atatgataat
     9961 tccatcgttt ctgtatcgtt cagtgatcag tgtcctcagt ggtaatcctg taagctgcca
    10021 tcggtaaata tcactccttt cagaaaaaag aaatcaacca cgaagcgcac gaaggaaaga
    10081 gaaggttgca atacaatctt cgtgttcttc gtgcccttcg tggtgaaaaa tcctgaactg
    10141 aaaaacagta agcggcgatc gcgccttatt actttatttc aaaataagat agacaaaagt
    10201 ttatattcat aatctaaatg gctaattcct aaattggaga gaagtatgtc tgctacacca
    10261 gaacaatcta taacaaattc aaaacaagtg gatgtagatc ctatcgaaac aaaagaatgg
    10321 cttgatgctt taaatggagt catcgatgcc gaagggactg atcgagctgc ctatcttatt
    10381 gacaggcaaa tcgaattcgc aagagttaat ggagtcgtgc aacccttcca cgcagaaacg
    10441 ccttatatca atacaattcc tgtggagcaa caatctcctc ttccgggaga tcaagcactc
    10501 gaaagaaaaa ttcgttctta tactcgttgg aatgctatgg ctatggttct tcgtgcaaat
    10561 gaaaattcaa atgtgggagg acatatttcc tcttttcaat ctgctgctac gttatacgac
    10621 attggttaca atcatttctg gcatgctcct tctgaaaaac atggaggaga tttaatttat
    10681 gttcaaggac attgtgcacc tggaatctac tctcgtgcct ttttactagg aagactcacg
    10741 gaagatcaac tcatcaactt tagacaggaa gtagatggaa gtggaatttc tagttatcct
    10801 catccttggc tcatgcctga cttctggcaa tttccaactg tgtctatggg acttggtcct
    10861 atcatggcaa tctaccaagc cagatttatg aaatacatgc aagacagagg tatgatcaaa
    10921 gaggaaaaca gaaaggtttg ggcgttctta ggagacggag aaaccgatga gcctgagtcg
    10981 ttaggtgcaa ttggaatggc tggtcgagaa aaactagaca atcttatttt tgtaatcaat
    11041 tgtaatctac aaagactaga tggtcctgtt cgtggaaatg gaagtatcat ccaagaacta
    11101 gaaagtgaat ttagaggtgc cggctggaat gtaatcaaag tgatttgggg acgtcactgg
    11161 gatgcgttat ttgccagaga caaaaaaggc attctcatga aaagacttgg cgaaattgta
    11221 gatggtgaat accaaaccat gagagcaaaa aacggagctt atatcagaga gcatgtattt
    11281 aatactccag agttaaaagc actagttgca gattggacag acgaagatat ttggaattta
    11341 aatcgaggtg gacatgatgt tcacaaagtt tacgcggcct atcatgcggc ggtcaaccac
    11401 aaggggcaac cgactgttat cctcgcaaag actgtaaagg gttactggat gggtagctct
    11461 gggcaagcaa tgaatattgc gcaccaacaa aaacacatga gtgatataga tgtaaaagca
    11521 tttagagatc attttggaat tccggttcca gatgataaat tagcagaact tccttttgta
    11581 aaatttcctg aaaatagtcc agagatgcaa tatatgaaag aaagaagaaa tgcacttggt
    11641 ggctatctcc ctcaaagaag aaaaaaagca gacagtctaa aagttccttc tctagatgca
    11701 tttgcgtccc tcttagaagc aaccactgaa ggaagagagc tttctactac gatggcattt
    11761 gttcgacttt taaatatttt actcaaagac aaagaaattg gaaaacgtat tgtgcctatc
    11821 gttccagatg agtctagaac ttttggaatg gaaggactct ttaggcaact cggaatttgg
    11881 agtcatgtag gacaactcta tactccacaa gacagagatc aattgatgtt ctatagagaa
    11941 gacaaacaag gacaagtctt acaggaagga atcaatgaag caggtgctat gagtgattgg
    12001 attgcagcgg ctactgccta ttcaactcat ggaactatga tgattccatt ttttattttc
    12061 tattctatgt tcggatttca aagggtgggc gatttatgtt gggcggcagg cgatatgcgc
    12121 agtagaggat tcctactcgg aggaacctct gggcgcacaa cgttaaacgg ggaaggactc
    12181 caacacgaag atggacacag tcatctctgg agtgcaacga ttcctaattg tgtgagctat
    12241 gatccaacat tcagctttga actagcagtt attttacaag atggactcaa aagaatgtat
    12301 gcagaccaag aagatatatt ttattatatc actgtgatga atgaaaacta cactcaccca
    12361 gctcttccaa aaggagaaga aagaaatatc ctaaaaggaa tgtattctct ttcaaaatca
    12421 gataaaaaag atgcaaaact aaaagtgcaa cttctaggaa gtggaactat ttttagagaa
    12481 gtcattgcag cagcagaatt actcaaaaac gattggggag tcgaggcaga tatttggggt
    12541 tgcccaagtt ttacagaact aggaagagat atcaatcgcg tgaatcgaaa caatttacta
    12601 aaccctgagg ggaaaacaga agttagccac gtagaagcct gtctcaaaga tacaaaaggt
    12661 ccagtgattg cggcaactga ctatgtaaga atgtttgcag aacaaattcg tcctgctctt
    12721 caaaacctag gaagacgata tacaacactt ggaaccgatg gatatggtcg ttctgataca
    12781 agagcaaagc ttagacattt ctttgaagta gataagagat gggtaacaat tgccgctctc
    12841 aaggctcttt ctgaggaagg cttgatcgaa cctaaaaaag tttcagaagc aatcaagaaa
    12901 tatggaatcg atcctaaaaa accaaatcca ttaacagtat aaaaggaatt tatatggcaa
    12961 aattaattga tgttaagatt cccgatatag gggattacag tgatataccg atcatcgaaa
    13021 ttcatgtgaa agtaggagac aaagtttccg cggaacaatc tctcatcaca ctcgaatctg
    13081 ataaagcaag tatggatgtt ccctctcccg caaatggagt ggtgaaagat ttaaaagtaa
    13141 aagtgggaga caaagtctcg aaagactttt tatttcttgt tttagaagga gaagaaggag
    13201 tagcagaagt caaagcgcct actcctgaaa aaccgaaaga gagtgcgcct atcgcacaat
    13261 ccgcacctcc accacaaaaa ccaatcactc ctccagtgca agtagaagtc tcgcatgact
    13321 caaatggaaa atctagtcat gcaagtccat ctgttcgaaa atttgcaaga cagcttggag
    13381 tagatgttgc aaaggtaaaa ggaacaggac ctaagggaag aatcacacaa gaagatgttc
    13441 aaaattttgt aaagtcagtc atgagtggac aaacttccac ttcttccaat acttctggac
    13501 tcaatctact tccttggcct tcgattgatt ttagtaaatt cggagaaaca aaacgtcagc
    13561 cgctttctag aattaaaaaa ctatcagcgg ctaatctttc tagaaattgg attgttattc
    13621 catctgtaac ataccacgaa gatgcagata tcacagacct agaaaaattc agagtagata
    13681 caaacaagca atacgagaaa gaaggaatca agctcacaat gctttctttt atcatcaagg
    13741 cttgtgttgt ggctcttaaa aaatttcccg agttcaatag ttctctagat ggagatgact
    13801 tagttttaaa acaatactat catattggat ttgctgcgga tacaccaaac ggcttagtcg
    13861 ttcccgtaat caaagatgca gataaaaaag gaatttttga aattgcaaaa gaagcatccg
    13921 aacttgcaaa actagcaaga gatggaaaaa tcaagtcaga acaaatgcaa ggagcttgct
    13981 ttacgatttc gtcgttaggt ggaattggag gaacttattt ctctccaatt atcaacgctc
    14041 ctgaggtcgc aatacttgga gtaagtaaag ccgccatcaa accagtttgg aatggaaaag
    14101 agtttttacc gagacttatc tgtccactat cgctcactgc agatcatcgt gtgattgatg
    14161 gagtccttgg cacaaagttc aatgtatatt tggcagagct tttgtccgat tttagaagag
    14221 ttgtattata gaggaaaata tggcagaaaa agaacttaaa attccagaca ttggagactt
    14281 tgacgaagtc cctattattg aagtttttat caaagcagga gatatcgttc aaaaagaaca
    14341 gcctctccta gtcctcgaat cagataaagc atcgatggag attccagccg aagaagcagg
    14401 taaaattcta tccgttgctg taaaagtcgg agacaaagta ggaaaaggaa aattgattgc
    14461 agtaattgaa gtttcagcca ctgcaaatac aaaccaaacg gctaatacga ataatcctcc
    14521 caaagcagat ccaccgaaag tgcagtcggc tactgctgtt ttaccgcaag gttcttattc
    14581 agggaaagta gatcatgaat gtgagatggt agttttaggt gcaggacccg gcggctacag
    14641 tgcggcattt agaagtgctg acctaggaat gaatacaatt ctaatagaac gttatccgac
    14701 tattggtgga gtttgcttaa atgtaggatg tattccatca aaagcacttc tgcatacagc
    14761 ggcagtcatg gaagaagtaa aggcaatggc gtcacacggt atcgtgtatg cagaacccaa
    14821 agtagatatt gataaacttc gccatcacaa agaaactgtg gtaggaaaac taactaacgg
    14881 tttagcagga atggctaaga tgcgaaaggt caaaatagtt cgtggtcttg gaaaatttat
    14941 agatgcaaat catattgaag tagatctcac agaaggaaca ggacaaaaaa caactggcaa
    15001 aaaagaaatc gttcgcttcc aaaaagctat cattgcagct ggaagtcaat ctgtgaaact
    15061 accttttctt ccagtggatc caagaattgt tgatagcact ggtgctcttc ttttaaaatc
    15121 aattccaaag agaatgcttg ttatcggtgg gggaatcatt ggtcttgaaa tggcaactgt
    15181 atacagcacg ttaggcactc ggattgatat cgctgaaatg atggatggtc ttatggctgg
    15241 agccgatcga gacttacaaa gagtatggga gagaaaaaac tcgcaccgct ttgataaagt
    15301 aatgcttaaa acaagagctt ccaaagcaga agcaaaagca gatggaatcc atgtcagttt
    15361 cgaaggagag aatgcaccct cttcgcctga ggtctatgat ttagttttag ttgcagtagg
    15421 tagaacgcct aacggtaaga aaatcgatgc tgaaaaagca ggagtccaag tgactgatag
    15481 aggttttatc aatgtagaca aacaacaaag aaccaatgtg agtcatatat tcgcaatcgg
    15541 tgacattgtt ggtcaaccta tgcttgctca caaagcagtg cacgaagctc atgttgcagc
    15601 agaagcagca ttcggagaaa aatcattctt tgatgcaaaa caaattcctt ccgttgccta
    15661 cacagaccca gaagtagcat gggcaggtct tacagaagag caatgcaaag aaaaaggaat
    15721 caaatacaaa aaaggacatt tcccttggtc agcaagcggt cgtgcgatcg ccaatgggcg
    15781 tgacgaaggt tttacaaaac tactctttga tgaagaaacc catcgcatcc tcggaggcgg
    15841 aattgtagga actaacgcag gagatctcat tggagaagtt tgtcttgcta tcgaaatggg
    15901 agcagatgca attgacatcg gaaaaacaat tcatcctcat cctacgctcg gcgaatccat
    15961 cggcatggca gccgaaatcg cccacggcac ttgcactgac gttcctcctc agaagaaata
    16021 aggggagagg attaccaccg ataaacaccg attcgcttcg ctgacaccga taagggatgc
    16081 gatagtttct ataggtgagt gtgtttattg gtgattgtgc tgagttctgc tagcaaaaaa
    16141 ggctgtcact cccgaaattt tctatcggga gtctcttgag ctagaaatcc cctgattctt
    16201 ctgggattgg cggataatac agagtagtcc cctttgcgaa aggggatact ttggagagga
    16261 aatgtagact atttgaattt gtttaacttt ggatgattga gaactaatga aaatgaaaag
    16321 acttcctaaa aatataaaga tttatttgag tttgtttgta gtgattgcat ttctttcaaa
    16381 ttgcagaacc cagcctgaga ttaaacctga ttcgcttaaa aaatcttccg ctgtcgtttt
    16441 tcagtttctt tataattatg aaaacctttt aatggatata ctgagttcga cagtgtttgg
    16501 attatgaaat tagaaaaatc ctctaagtca tattccgatt taaattgtat agaagcaaac
    16561 tacaaatcaa ataaaagatt ttatttttta aatattgaac ctgggcagta tactattgtt
    16621 gcagggaaat tcgggtatta tcgcactcag tcaaaaagtc cccgctataa tttctcaatg
    16681 gtgtttgata aagaatctag agaaaagatg aagtttaccg taaaagaaag tgagctaatc
    16741 aatctaggcg aatttttaat ggatgaaagt gcagttagtt tcgaccaaga tccagacctt
    16801 ggcagaatcg gcgaaaaaat attcccagga tttaaaaata attttggaaa aaatgtgcaa
    16861 atgactatga ctcccggact atatcaagca ttggaattac ccgctatagg gaaaaccttc
    16921 aatcatgaca atcgcccaga gaaactagaa gagattagaa acgaagtcaa agaagacttt
    16981 agcggaactg aatggaaaat tttcgtaaag taaatttatc attacttaag aggctactcc
    17041 ccattagccg caaataagaa aaacgaagat gacctatccg aaattattcc cttcaaacaa
    17101 tccacccaca tagcccttga ctaaaactcc attgcataaa aattactcga ctattcatac
    17161 aaatacaagc aatgtagtgt ataaaataaa taacgccagt tcgatgtatc atataaatta
    17221 aaagattacc accgattaga acgattaaca ccgataaaag atacaataat cataaataga
    17281 tccaaagatt ctttctcgtt ctgttgtcgg tgtgtatcgg tggtaatcct ctagctgaca
    17341 ccattgacca acaagctcca ctgtttcaac tttacgcaca cgcaaagtga attcagaaat
    17401 attttttgat tgcagttaaa ctaacactgt taaactagac ttatgctatt ctattataaa
    17461 cagcatccag gaaaaaaatg gtttgcttct aacttactcg ctctcattgg tcgcattgct
    17521 tccgttctat tgtataaagt aaaaataatt tctactccgg atttaaaaat agatggacct
    17581 gtattaattc tagcaaagca ctcgattgaa atcgatatcc cactcggtta ctatgctatg
    17641 cttgtagcca ttggcagaca tgcttggtgt atcatgaaag caagtcttgc gcgaagtcgg
    17701 tatttaggtt tctactggaa aatcggcggt atccccttag atcgtgataa accagaaaag
    17761 agcaaacaca tgttagtcta tgcaaagaaa gttctatatg atctagatga aagtggactg
    17821 aaaaaaggaa atggaaatgt aatggtttta tttcctgaag agacaacgta ttggcaaaca
    17881 atgggtgaag gacaagcagc agggtttaga tttattgtgg gtaagcctgc tattccgctt
    17941 aacgtagtat gcgccggttt cagatacaca aaaggtttta tccgaacgaa tctagaaatt
    18001 gagtttggtc ccattcgaca atttacaaag gatgatgacc atgctagttt cctgcacgat
    18061 agaatggttg agattgcaga attgtccaat ctcccctatc cattctctca accaaaatca
    18121 agagctaaag tccttaccga agcaagcgct tccaattaaa ttttactcct tgcggaatgc
    18181 ggctaatttc tatttgccgc ttcactcttt tttttaaata ctgttatatc cgtctcttgc
    18241 ttcacttgtt tgtttttaac taaagattca aattcaattt ctttctattt aaatttaaac
    18301 acagagatcg gatcctaacg atttttaagc ttaatcaaaa agatttacta gagacaccta
    18361 agatggagac ataggttttc actggttatt ttagattatt acaagagcta aagtatttat
    18421 aagtagttca atttatatag aatcccattg acagttaata acaaaggttt actcctgtac
    18481 aaaataggtg tttataccag atttggtttt atgaaaagga attcaatgtt tatcaatttc
    18541 attaaaattt ttcctttcaa taccatagta agaaaaatgc aagcgatctg attttattag
    18601 atataggaca aagctttcaa cagtaaattg cggattaggt gtttaaaaga tgatacaaaa
    18661 taatttcagg cactatttga agattagaaa ttatttcttt gcgttattct ctacagttct
    18721 tattcactta acttgctccc tctcggacaa accgaaagta ttaggttgga ttccttttaa
    18781 accgccgacc acaccagcac aaatttttat caattctcca gcaattaaag ccaccgcaga
    18841 agatggaatc tctttacaaa tacgctttag tttaaataaa tctccatctt cacaagtgag
    18901 tattcaaatc acaaattcca atccaaatga agcttccatc tctacaacta acctcaattt
    18961 tacttcttca aattgggata ctgttcaaat cttaactgtc acaggcatag atgatcttta
    19021 cgtagatggc gatagaaaat ataatataaa ttttaaaatt acatcagatg atctagaatt
    19081 ttctaatctc ctattagatc ctatcgagat tatcaataaa gacaatgaca ctccaggagt
    19141 tcaagtgact ccaagaactg gacatcttac atcagaatca ggtgccacta ctactttcca
    19201 agtttttcta tctagtgcgc caactgcaac tgttactatt ccaactatcg caagttctaa
    19261 tacgttagaa ggaactgtga gcccatctac tcttactttt acaccaacga actggagtat
    19321 tcctcaaaca gtaacgatta ctggagtgga tgattctata gatgatggaa atcagaatta
    19381 tagtattgtt tttggaacga caaccagttc tgatccagtt tacaataact ttgtaacaac
    19441 aaatctaaat gtaattaatg ctgataatga gactgggagt gggatcactg taagtggctt
    19501 aagcggaaat acaaccgagg taggtggaac tgctaccttt aatgtaatcc taaatacaga
    19561 tccaacttct agtgttacaa ttcagatttc ctcttctaat acaaatgagg gaacagtctc
    19621 cccaaattca cttacgttta attctgggaa ttggaacatc actcaaactg ttacgattac
    19681 cggagtaaat gatctaatac aagatggaaa cgttagttat aatataatca taggtggagt
    19741 aactagtgca aactctaatt atgctggatt agcaaatcaa actctttctc tttccaatac
    19801 tgataatgat acagcaggct atacaattaa tacaacgaat ccttttttag ttacagatgg
    19861 aggacaaatt acctcttcca ttcaaattag tttaaactcg caacctacaa gcaatgtaac
    19921 aattcctatt gctagtctta atacagcaga agcaaatgtt tctgtaagta gtgtaacgtt
    19981 tactccatcc aattggaata ttgttcagac tattttagta agtggagtaa atgatgggac
    20041 tgttgatggc aatcaagcat ttacaattga tcttgcaatt ccaactacct ctgatagtgt
    20101 ttatgctgct ctgaatcctg ccaatcaatc tggtaatagc tgtgataatg atgggactgg
    20161 agttttaatt tctgtttgta gaaccaattc tactttaaca acaacagaaa gcggtggtgc
    20221 attaacattt tatttattac tttctcaaag tccaactgcg aatgtaaata taggattatc
    20281 gtctagcaat acatcagagg gaaccgttag tccagccaca ataactttga atacttctaa
    20341 ttggaatact ttttcgggtt ccaatttaat tactgttaca gggatagacg attcaaatta
    20401 tgatggagat attagttata atttgaattt tgcaactagt tctagcacgg atcttgtttt
    20461 taatggaatc aatctctcac ctattggatt ggtaaaccaa gataatgaaa tttattttaa
    20521 tgtgtcagca gtgactggtt ctccgatggt agaaaatatt ataggtggaa ctgcctcttt
    20581 tacaattgca ttaaatagtg cgccttctgg aataaataca gttagtattg gaatatcgag
    20641 cagtgatgca acagagggaa ctgtttctcc ttctagtgta gtttttaatt ctggaaactg
    20701 gaatactcca caaaccataa cagtaacccc agttgatgat accattgcag atgggaatat
    20761 atcgttcacc attttaactg catctgccgt tagtggggat ataagattca atggtagaaa
    20821 tccagctgat agaactctag tcacaaatga tgcaggagaa aaaagaactt ttctcacatt
    20881 tacaacctat aatggaaatt tgggaggtat cgcaggtgca gatacgaagt gtaatggaga
    20941 cacaaatcgt ccaagtataa cacctaatac ctatagagcg ttagtctccg tcaataccac
    21001 tcgaacagga aatccattag ctggatcttg gtcattcgca gcaaatacaa cctatttcag
    21061 aacgaacgga actacaaatg tctttccttc ggatgctggt tctatttttt catttggggg
    21121 tttaacaaat tcttttagtg gtgcaagcga tgaatactgg actggactca atacgaattg
    21181 gactgttggt agtaactgca cagcgtggac aagtagttca aatgctataa gcgctcgata
    21241 tggtattgga aatacaacaa atactgatag cattagcatt ggactagata cctgtgataa
    21301 gtttaaaaga attctatgtg tgcaacaata gatttaaatt ttacaacgga aattctattg
    21361 acgatttatt cttaattctc caaaatgaaa gaatacaaat acatccaaag aatgaaatta
    21421 cttacgcaag aacctaaaaa agaaactata gattgtattg attttttaat cagtcaatag
    21481 cgttaaatta taagagcaga atctttttaa gtatcgagat aggaacttgg cttccaagga
    21541 ctcgatttcc ctcctgatga cagactaaaa tttctagaat ttttttccca aatacttttt
    21601 taacttcagg ataattttta taagaactaa cgtatgctgg agagacatta gattctataa
    21661 acataaagta aatcgaatct ttttaaatca agaaatcaat ttcttctttg tccaaaatga
    21721 agtgtgattt tggacaagaa taaaattaga attcagaaag atctatgtag caaaaggcat
    21781 cgtattcaat tctgcccaaa gttcccctaa acttaggagt aaagattggg taaattgccc
    21841 ctaagcttcc tcgttaggga actttaccct aagtttaatg gatctttagt ggaagtttag
    21901 tatatagaaa ttgaaaagca cacggaatgg aaagttacga ataagggttg acttagttca
    21961 gtaacttagc taaccctaaa attatgagca cagtattgaa tatacatgaa gcaaaaacaa
    22021 atctgtccaa gctcctttca aaagtcatgc agggagaaga agtaattatt tctaaacaat
    22081 atattttaga cactcataca tttttatggg caatcgcgga accagaatcc atttgatagg
    22141 ttacttatcg cacagtcatt agaggaaaat actccactta taacaaatga tccaatcata
    22201 aaaaaatata taccaaatga aaaaagtaat ttcccacacg gactttattt gagtagcatt
    22261 tggtataccg aacttactga tgttcgggaa cactaagaag tgtttagacc aaaaccactt
    22321 tattattttt gaaatgggat ttttcttttg acgtttggta taaatcgaaa ataatatggt
    22381 aattatctga aaaaatacaa aactatttta tatgaatgca gctaatactc gtttgccaca
    22441 tcaatgagaa agatttgcca aataaatcga ataaaaataa aatacgataa aggaaataaa
    22501 atgaaaaaac tcagttattt actagtctta ctcttcactc attgttttac agtttatact
    22561 caaaacgaat ctattttaga atcggaaaaa ataaattcca aaacgcaaat agagaaaaaa
    22621 gatttatatg ttttaacgca tacattaacg aatgatggtc tttatctttt gctagatgca
    22681 taccaagtag aggaagaaaa ccaagttcat tttattaaag aaaaatacga agaaaacaaa
    22741 ggagtaaaat taaaaactat tttcgacgac gggattggag gatgcgatgt gcaactttgt
    22801 ggcgcaattt tagtagtcac tacaaccatc tttctattag aaattccaac tctacccttt
    22861 cgtatgatgt cagaacccaa agaaagaatt cgaaaagaaa ttaaaacaat gaaatcaaat
    22921 aagataggcg ttctatctac agaaaatatt tatttttcct ttttagaaaa gaattcatct
    22981 tttaaaaatt atccatttga agaagacaaa gtattgattc caatctctga acttggtcta
    23041 aactctttta acataaagaa tttttcttat aaactaacct tcacatcgaa tggtaaaaaa
    23101 ttaagagagg gaaatttaga tttatcaaat gatgttaaaa agagcaaaga actgcaagca
    23161 atcgtcaagg aaaacgattc taaaaatcct gctgttcaat gtaaaatatc gtttcctatc
    23221 ttagagaaac aagacttgga aaaaaatatc gccgaaaaag attgcacaga gacctgcaaa
    23281 cgcaaattct cgaaaccaaa tgtattggtg tggaataacg atgaatacaa tgcatgttta
    23341 aaatcaatag agaactgcta ttccattact aggattaaaa aataagacta gaaatatttt
    23401 tcaatcgata atttttatag ttggcactct aattaataat tttttactaa ggaaggatag
    23461 ttaaaaaaga ttctaagtga attataattt aagcttttac aaattatatg aagcgtataa
    23521 aatataccta tgaaatattg tttacttgca attatttttg tgcaaattca atgtgctagt
    23581 tttaaagtat atgaactagc atttccaacg agcgtcaata gtgcgtccaa atcaaatatt
    23641 gactctgtca aattattaga aaataattct attgaattaa aagttaaaac gttattatcc
    23701 acaagtccta aatgtattga actcattcaa acttctcaat attattcagt ctattcctat
    23761 tcttttaaaa attgctccct atcagatgca agaaaagatt tggaacaaga aaaaaatacg
    23821 aacccagcta ttttaccaat cttagatcaa agaaagacag acattaattt atcagaatac
    23881 gatgcctaca gtaaatactc agattattta ccctatgttc aagagtattt aaatgaaatt
    23941 caaacaaaga aggtttatat caataacaaa aaagataaaa tttatttaac aagtgggggc
    24001 aaatattgca ttatcctcaa tagagatgct aggtatccaa gtgtatcaaa ttataaagaa
    24061 aatcccggta attgtcccgc atcctatcca cagagcgatt ttaaagaaat caatgtcttt
    24121 caggcagaaa cagcaaaatt gtatttagat tttcatgatg gaagacttgt ttatacttta
    24181 ccaaaatcaa atctacgaag catttattta aatatagtag aattaaatag agacgataat
    24241 tatttttatc tactacttat gccaacagcg cttgtctttg atatagttac atttcctttt
    24301 caactggttt atttttgggc attaggaaat gcgatacccg ctcattaagg ccagccaatt
    24361 aaatttgatt tttctttttc ggatagttca gaaattttta atctgtatct tcctacttca
    24421 tcgcctgacg ttaccatgat atatattaga cctttttgaa ctattgttcc tgtatcatca
    24481 tcctttgata aaaatttctg attttgatag gtaaatgctc ttggatcaaa agaattattt
    24541 ttttcattcg aatctttttt tatttcaatc ctaattccag ttttaagaga attctcaaat
    24601 ttataaaagt cggcataaga cccatcggga atttgagtat tcgatttatt tatattacct
    24661 aagatttcct ctccaatctg aacttttttt gaatgctctg attgacttac ttcagagatc
    24721 acggggtttt ctctctcgaa gactccgaaa tttaggaagg actcgtaagt cccatgacct
    24781 ccggtatatc cttccgtata tgtaaaaaaa atctttgaac tacatagaaa gcctttgcct
    24841 ttcccatcta taaataaata ccctctccca cgacttctag aactatcagg aaataaatcc
    24901 acatcaatct tagtaaattg catttcagct attggttgcc aaccgcaaat cgcaagtgtt
    24961 aaatcagagc ctttagctga aaattgagat ttttcaagat ttttaatggt tccctttatt
    25021 tcgcctgacg tcataatgca ggcagtaaaa tatactcctg ttaaaataat aactgtgatt
    25081 ctaattagat ttttcatgat ctgtgataga ctaaattcta tctttccaaa aaaggaatgt
    25141 aaaggattta ttctttatgt tcgtaatttt ttcttttgca cccgtattta tatcaacaat
    25201 ataaatatct aaatcgcagt gaacatttcc tccctcatct cgattgcaat ttccaggttc
    25261 ggatgcaata tacgctatct tggaaccatc aggcgaccaa gtaaattctt ctacaccact
    25321 atagccctct cctgaaaaaa taaatcctgg tggctcatag gcttttagct gaagtaaatc
    25381 tttatctgtt ttctcactca gcaaataaat agaaaatgga ggatataaac cttcttccgc
    25441 cttcattcga aataaaattc caagttctcc atttgtagac cattttatgt cttttgtata
    25501 atcaggcata tttttttgta taagaatttt gccggacgtt agatcaatga ttcgaactga
    25561 gtattgaata ggattccata ctttatctaa ttctatggtt gctagtttag ttcgatctgg
    25621 actcaaaata aaagaactga taaattgttt tgatttataa agagtctctt ctttatttga
    25681 actcaaatcg attgtttgga ttgattgacc tggcttatcc tttcgatctg ataaaaaagc
    25741 tactttatca tccttaaacc attctgctct ctcatgttct attttgattt ctttcttttt
    25801 ttttccattc aaatcaaaaa ctttgataga actcaagtca ctgtataaaa ttttatcttt
    25861 tttatcattt agaattggta attgatataa aattgtattc tgcggataat ctaggttacc
    25921 ctccttattt ctaagcttta tctttttacc attttcaaaa attagatact ctctatattt
    25981 aaaatagttt agcgaagaaa tctgagtttc atcaagtaaa tccaataact ctactttttt
    26041 gttttctgga gttactataa aaacttccat tttatcattg tagttttcag gatcaatttt
    26101 tccggagact agaataaaat ccttatttct cggaagtgaa gtcttttcat ataattgttt
    26161 atttagacaa ttttttaaga ataagcagga caaaatgaca agaattgttt ttttcattct
    26221 tttatctccc ttggatcttt gaagaaaaag tatttatcct tttcgattcc gctgaagact
    26281 ctttttattc tttcttctag attttcgtca taagcaactc gaatgtaaat tttcttgatt
    26341 tgatctggat attttcgaaa gagtgtcgca taggcctccg gatctttttc gccggaatct
    26401 ccaactaaaa taaacttcac atttttccac tctttcaaaa taggttcaat cttttgtatt
    26461 ttataattgt cagggctttc aaataagttt aaaacactcc aatcttttaa tcgaatatct
    26521 ttcataaaat aaattgattc aggaaacttc tcgtctaaaa aaaatttgga aagatgatta
    26581 tacatctgcc aaggactagc actcacaaat aaaaagcaac taacgttact tgtaagactt
    26641 atataataat cattcattcc acttattttc tcaaaaggtt ttataaaact attttctaat
    26701 aatttctttt tgtttctaac atcgcttagt ttaatggtat catccacatc cgagattaaa
    26761 caggttgaat cttctggtat gatccagatt tttcctttat actctttttt atttctagag
    26821 gatgaattta catttacttc tatttgattt gtattttcta aaatagattt cttgggaatt
    26881 ttaatttcgg tgatagagcg tccattggct tctgtttcct tcaacttata atcttgccct
    26941 aaaacacgca cagaaacttt cttccctctt ttattgtcta ctagaaatga cctaattctc
    27001 tcttgaaata ttttagaatt gtcttcatca gatactttta aatacttttc caattcttgg
    27061 atcaagattt ttcttttaat cgaattttct tttttcttaa aaacatgaag atgaattttt
    27121 accttatatt cagaagctga tttatctaaa tatagatacg ttgggtataa aatcacatct
    27181 tcgtcttctt ttaattcaga actaaacata gtataagaat taaaaaaaac tattctaatt
    27241 aacaacagtt ttattttaaa tttcatttta aaaagaggca gttacatcaa ataaaaatct
    27301 tctaccttct cgaatataat caaacggaaa attattattc tttaaaagtg tttgatgtga
    27361 attaaacaaa ttcataacat ttatacccat ttcaacagat tcattccatt tatacatgaa
    27421 tctataatta acattgatcc atgcaggaac cactttggga cgatagacgg gataattgta
    27481 ttcataagga ggaagatttg tattaaaacc aaatatagga tcgacaggac ccaaatcact
    27541 attcctccga taaacagaac cttgcctttc aaaggaaata ctagtcataa atttttgatc
    27601 tgtccaagta atacctaaat ttgcagtatg acttggaacc caagtgattt gattcttatc
    27661 gatagaaata gttctatctt gtataaattc attgagtctc tttgcatacg aaaaatttaa
    27721 gaaaccagaa agatttttat aggtaaataa cgcttctccc tctgccccac ccgttgtcag
    27781 tgtataaagg ttagtgctta agttattatt tgccacgcta tacgcaattt gattttcata
    27841 tcttgttcta aaaagatttc ctcggatgtt aatgtatttg tttataagcc aatcaacgcc
    27901 aacctctgtt gtatgaatca cctcaggttt taattttctt ggattggatg caagagaaaa
    27961 tgtattaaaa ccaaataact ctgtaataga tggctctcta aatgcttttc ctgacattaa
    28021 cttcaaagtt aaattatttg aaacaaagta tacgaaaccg acccttgggc tcgttctctt
    28081 aaaaattctt ttctcgtttg gaacaaatgg atctcctaaa tattgagagg gaataggagt
    28141 gccatctaac gaaggtgttc ccaacagtcc agatctaggt tgatctactc ctctaaactt
    28201 ggcttgagtt tcatcatagc ggataccaaa tgttatctcc aatgaacgat ttaaaatttt
    28261 tccagaggtg atttgaccaa aagcagccat tttcggtatc ggtttatttt taatccattc
    28321 taaccaaggt ccttgtttaa cataaccacc actagcataa ggtggaaatg cattaactga
    28381 atcacttaaa ttaatattac tgtagtgttc actatctcca gtatatacaa atcgatttgc
    28441 ttccacccca ccgagagcag ttcctccatt tggaaatatg taggttaact gacctcttcc
    28501 aaatatatca ttggcaccag tctttaaata ttcagttaca ccacctggat aatatccatc
    28561 atacgctcca ttttccgcat aacgtgtatt ccaatcaatg gatctgtttt gatagcgaat
    28621 tgcatactct tggattaatt tctcagttat atttttagta tacttcattg ttacaatttg
    28681 tctggtttca gccattgctt ctttcacatc cggagttctc caaagccatc cctggcctgt
    28741 ttgaaaactc caatactgtc tatgatattg aattgttaat cccttcaaag caccctcacc
    28801 ttccaatttt gtgaagagat aattactatt tcttgtatca ttggttgtaa acttttgaag
    28861 gtaatttgta aaaacaggat ctgttcttcc agacccatca taatttttta cattatttcc
    28921 atctgtctga taggaattat aacttactac ataggaaaaa agatttcctt tgtttcctgt
    28981 aatgatgtca taaatacgag tcccaaaatt tccaccacga attctagttt ttaaagatcc
    29041 gcctagatct gatccagaaa aagtattaat tgaaacaaca ccgttagtcg cattagatcc
    29101 gtaaagagca gatccaggtc cacgaatcac ttcaagtgac tttatcatat taattggagt
    29161 tatttcagat gtataagcac tcccatataa attatcatta aattggattc catctacaag
    29221 cataagtaaa tggttgctat tccatccttc aaaaactcca cgagagctta ctgtccgtct
    29281 atcataatct tgtgagggag aaaatccagg aagttgatag agtatgtcat tgatactaac
    29341 tcgcccataa tcttggatct ctttatgaga aataacagat acaatatttg gagcttcact
    29401 tgtctttctt gcaatctttg tggcggatgt tgtaacttga ttttgtaaaa gattaaatac
    29461 ttctgcagat gcttcctcat ctgctttcga attttctctc actggtagtt tattcttttc
    29521 agtaatccca tttgcaattg cattttgatt tatattttct ctattctctt ttctattatt
    29581 attcgaacga atgctattcg atgttttatt tgcaattttt tggatgattg tttcatcttt
    29641 ttctttaagt tctttggcat ccaattgaac tccatctact atatcgagtt catcgacaat
    29701 actaattgca tcaatcatat aacccgtatt tggattgtaa atttggatga atacagataa
    29761 atttttagtt tcagttcctg atgtataaaa accttcaatc aaaaaggctg catctttttg
    29821 ttttgcataa ttcatattat cctgtaaact tccacttgcc actgtcactt gaaatttatt
    29881 cgattccaaa ttagatttca ttgattcttt gatttggttt tctattgctg gatttttttc
    29941 agatttgtat ggattaaaat ctccaaggac aacaacttta gaaagactcc cttcttttgt
    30001 ctgagagtaa agggaaaagg aaagcaagga cacaaaaatg ataacaattc taaaactcaa
    30061 gataattctc cattcagtgt agtaagtttt tttgtaatca acccggatcc gcttcaaaaa
    30121 aaacgcactt agtctaccga caaaatgaaa taagcaaaca atagggatta agaaattcct
    30181 cttatcagcc tatctaaatt cacctatatg gatatttttt taaatagaat tcaagttaat
    30241 cattagtaat ttaagagtta accattgcat gatttttact ccatgtaacg gcaaaggatt
    30301 gttatatgat aaatattgta tttgaaatac aaacaaaaaa ttgagacgaa atagaaaaag
    30361 aatgaatcag tctatggaag tgaacagaag ttaaatgaag ataattcttt attaagagag
    30421 aagataagag agggagtaaa atcttaacct tgaaattttt aaaaaggaaa aatgaatttc
    30481 gatactttac gagactaaag tatttctggt taaggtttta cccaatctta aactgttatt
    30541 tttgactaaa tctcatagcc atttggaatg gcacttctac gaggaacaac aataatacca
    30601 tctataattt tcacaaaatc atcctcgtaa ttctcatggt tttctttatt gataaggcga
    30661 acattgttcc caatataaca atccttatcg ataatagcat gcttgatttc acagtctttt
    30721 ccaattccaa caggaattcc gaatcctttt ttatcaaagg atccatacga atcagcaccc
    30781 atcacgacgg aattttctat atgggttcca gaatctacca cttgtcgaac tcctaagata
    30841 gagcgagtta tgttcgattc attgataatg caaccttccg atagtaaaac tttgtttaaa
    30901 tgcgctccat ttactttggt tggaggtaga gaacgagctc tagtataaaa aggtgtgctt
    30961 tctaaatata aattgaactt tggaaaatca tcggtcaaca taagattcgc ctcataaaaa
    31021 gctcgaatcg ttccaatatc ttcccaatat ccgtcataca cataggtttt aactttatga
    31081 gtttttagtg cgcctggaat gatttccttt ccaaaatcag tgagacttgt atctgcgagt
    31141 aattccttta aagtgcttgt attgaataaa taaataccca tagaagctaa gaaggttcta
    31201 tttggaagac ggaaggattc aacttctcca atgtcctgtg gcttttcata aaatccagta
    31261 gtccaattgt cttcatcttt tcttaaaatt ccaagagctg gtgctaaatc ctctgttaca
    31321 ggagttgctg caatcgtcat ttgattgctt ggatcagata aatgagtgat taaaaactga
    31381 ttgagatcca tattgtagag ctgatcgccc gaaagaatga tgatataatc ggatttataa
    31441 tcttcaatat gaggtaatac ttttcgaact gcatctgcag ttccttcaaa ccatcgaact
    31501 gaggacactg tctgctcagc agcaatgatt tccacgaagc cttgtttatg aatcgattga
    31561 aaattataag ttctagagat atgcctgttt aaagagtatg agttgaattg agtcaaaaca
    31621 aaaatttgct cgaatccact gttaagagca ttggaaattg gtatgtcaac taatcgatat
    31681 ttcccaccaa agctcaccgc tggtttagat cgtttctctg taagaggtaa aagtctagtt
    31741 ccttttcctc cacctaaaat caatacaagt acttttttct gtatctttga cgttatttgc
    31801 atttgatgat taacctatcc tatcttgttt tacattcgtc tatttattag cttaaaaaag
    31861 caaaaaaaat ttcaatattg aaactctatt ttcaaatgat attaatcata tttacaattg
    31921 aaactacata ctaaaaaatt catgaaattt gcaatttcat aagataaacg aaattaaaac
    31981 gcaatcttat ctaaattgat ttttttctca aatcaatgca aagaaagaat caaaaataat
    32041 tttcccatct gtgctgccag agatttcctc cacagctctt tcaggatgag gcatgagtcc
    32101 agcaatttta aaatccttgg atgtaattcc agcgatatct tctaaagagc catttggatt
    32161 ttcacctgag tatttaaaca aaatcctgtt ttcatcctct agtctcttta atgtttcctt
    32221 atctgcaaaa tatgaaccct ctccatgtga aattggaata tccaataact tgtctggaga
    32281 aattttttta gtaagtggat tttcttttga gccaactttt aaatttacat ttttacatat
    32341 atgctttaac gttttatttc gagtcaatgc tcccggaaga agacctgctt ctacgaggat
    32401 ttgaaaaccg ttacaaattc ctaaaacaag tctacctgaa tctagatggt ctttgagtga
    32461 aatcattgct ggagaaaaac gtgccagtgc accgcatctt aaataatccc cgtatgaaaa
    32521 tcctccgggc agaactacta aatcatattt tttttcaaaa ctatctttgt gccatacata
    32581 ggtcacatca tgatgatagt attttgaaag tatatcagca atatctctat cacaattgga
    32641 tccaggaaag cggataacgg ctgttttcat tcaggaatta cctctaattt gtaggtttct
    32701 atcacactgt ttactaataa ttttttacaa agtctatctg catcttctct ggctgtttca
    32761 atatttttag agtttaattt aatctcaatg tatttaccaa ttcgtaaatc agagaccatt
    32821 tcttctccca tatcatgtaa aacttttttc acagtagttc cctgtggatc caaaacagat
    32881 tcttttaatg taatatttac ttttccgata aacatctttt tatctttccc tgtaattctt
    32941 tatattttaa tattaattca tttataatac tatctggcag tttgggtggg ggtggatttt
    33001 tattccattc aattttttct agatagttac gaagaatttg cttatcataa ctaggcggag
    33061 atgacccaat tgcatatgtg tttaattccc aataacgaga agaatctggt gttaaaagct
    33121 catcaattag aatgacttta tcattgtaaa aaccaaactc aaacttcgta tcacataaaa
    33181 tcaaaccctc tgtttttact ctttcccgcc ccccaagata gagcttcata gagtaatcct
    33241 ttaaaatggt aaataactcc aaaccgattt tatcttttaa atctttttca ctgatatttt
    33301 catcatgacc tgtatcgttt ttgatggctg gagtaaagag aggttctgat aattcttctg
    33361 actcttgata tttacgacta acgtcgactc ctgcaatttg tcctgttgac ttatattcct
    33421 tatacgcaga tccacttaga tatcctctga caacgcattc atagtcgatt cgtttacatt
    33481 tttttactag aacggatcta tcttttaatt cctcacgtcc tttgtattcc ccaggaaatt
    33541 gattgtaatc tgtttccact atatgatttg gaatatcaga aaaatattta aaccaaagag
    33601 tagaaatgtt atttagtatt ttacctttgt cgggcactat ttcattaaaa acgcagtcaa
    33661 acgcagaaat tctatcggtt gaggataata ttaaataatc ccctaaatca taaacatctc
    33721 ttaccttacc tttatatgca actttcattt gattcattcc atctcccctc ttacttgctc
    33781 taattttgaa tctaactttt cttttctgta tataattaaa aataaaatca gaaataaaat
    33841 atgataggct aaaacattta cccaaaaagt ttccttcata tctttatcca taccacctct
    33901 tcctaaaaca gaaccaggat ggtttcccgg attttcaatc cagcgtatgg ctccccatgt
    33961 tagcaaagcg tttaacgcac aaagcaaact tagaaaggaa gaatataaat attttttatt
    34021 tcgatctgta attaaacttc taaaaacaaa ataggagagt aaacttacga caagtataaa
    34081 aaaggattga agccttgcat ccgtccagtc ccaaggagtc ccccaagcac tataagccca
    34141 aataggtcct gaaattaaaa ctaggatcga aaataaaaga gaaagtttat ttaagctata
    34201 agatagcgta tcccaatctg gattttttcg gacaatatag attaatccaa agagtgctga
    34261 taatgctgga gcatacaagg caacccaagc aacagataca tggaaataaa aaattctatg
    34321 ggataatcct tgttccaaaa ttacattcgg ataatagaga gaaagtatta ccgcaaaagg
    34381 aaaaaataaa acaaatagga caaataaaac tatgtctagc ataggagaaa aaattcttat
    34441 tttttcattc ataaatcact ccattgttat ttaaaaaaat catatatctt ctcctacttc
    34501 tcgaatcaaa agtcctagtg caagatataa gatcataaaa gcaaatagga caattaaaga
    34561 aggaaaatta atttcagcgg atgaaaagta tttcctttca gcttgcatgc ccgaaagtaa
    34621 aacagggata gaaagtggta tcaacaagat aggaagaacg atttctttca aacgtgtagc
    34681 aaaactgagt tgagatagag ttacccctaa aaaggaaaga gataaagtcc caggaattaa
    34741 gtagagaaat tgccctatca atttactaga ctcaaagtaa aatttttcaa aaaataaaaa
    34801 taaaagtaaa agtaaaaata actcaactaa aaataataaa aagaacaaaa caaatgattt
    34861 tgcaataaat tcggaatggg aagagatata gagtcttgta atacgatagg cacccgactc
    34921 tctctcttca taggtcactt gtgaaatcaa tacaaaagcg agtaaaaaaa tacccgccca
    34981 tttgagtcca ataatggaat caatttctag ttttgtattt gtctcaagag aataatgaaa
    35041 aataaaaaga aatgccatta aaagagagac tagagataga agtccatttt tagcacgacc
    35101 aatcaacaaa aattctttcc atagcaagtg cctaaacata gttaatttct ccattataaa
    35161 tatgaaggcg atttttgact agcttagaga gcttatcgat ttcatgagag actagaagta
    35221 tcgcggaatc tttaaaatct gtttttaaga tatttaagaa tgcttctgct gaattcacat
    35281 cgagtccatt taaaggttca tctaataaaa gtagaagtgg tcgtgcaaga agtgctcgaa
    35341 tgattccaga ttttttcttc attccttctg aaaatgttga gattggatca tccaagcgtt
    35401 taatcaagtt tagccgctca agtaaaatat ttaggtctga ttcaggcatt ggatttttta
    35461 aaatccgatt aaaaaaattt agattctccc gcaatgtaag agaactgtag agtccgtttt
    35521 catgacctaa gtaggatata gggttgatat tttttgaatt aaaattatca ttccactgaa
    35581 attcctttcg cttagaagac tgtaaaatca attttaataa gctactttta ccactcccat
    35641 tttcacccgt tagtaaagta aactcagaag caaaaatttc aaaggaaata cttttaaaaa
    35701 ttttcttata tccgatagta taagagagat ttttacaact gagaatggaa gttttcatag
    35761 aaaaaagtct aaggtcagga ttctagttta tgttatattt tcaattgcta tttttactct
    35821 ttgtattcca gatttttcca caaactatag taattgacga tgaaaaattc caaatgaatc
    35881 tatgggatca agaattagat caaaagcaaa aagttgattt taccaaaggt agtttcttaa
    35941 aaaatgaaaa cgactttatt ctttgcaaag gaagaatgtg gtctggaaat cccccaaaat
    36001 ctacttacct atttacctat cgagagaaac aaatcgataa tattggacaa tttaacaacg
    36061 aagcaataga actgattgag agaaatgaag aaggtgactg gtctgaggct aacgcaatgt
    36121 tatccgctat gatccaagct gatccaatgt tttttccagc tcgttataac tatgcaagat
    36181 ttctatttat acagaagaaa tttcaagaag caagttatga attcttacag gctcgaaata
    36241 ttatccctgc ctactatcgc agttatcttc acttaggaca tatttacctt tttctaggag
    36301 aaaaacaaaa agcagaagaa atctataaag aagccgcttg gagaaatcct ttaaacgatg
    36361 aagctcatat ttctctagct gaaatctatt ggaaagaagg tctcataaca agagctagag
    36421 atagaatcaa agaagctgat aaaacaaacg atccaacaga agcaacaatt atcagaagac
    36481 aaaaaccggc atttcctgtt cttgatttta caaaagcaat agaaaatcca ctcgcaaatc
    36541 caaatccgaa aattgcagag gctctattgt attttcatca agaaaaatat gcgttagcct
    36601 ataagctatt taaatcaatc aatacagaac atttaaataa ggagaaaatc ccttatctaa
    36661 aaaaaatgca ctattacttc gcagagactg caatcaaatt actagattat aaaacggcaa
    36721 cctctcaata tgacgcaatg ttaaagtttc cctttgatcc tttttttcaa gaagtttcaa
    36781 tggcaactgt tgaaagaaag aaaaatctaa cagctagtat taagcaaaac tccgactaac
    36841 agtagttaca tttgcttttc gcattttctt tttaattcat aacaaaatta actttcttga
    36901 ctctttccaa ttttactgta tttctatcag tatgtttttt ttccaattac ttaaaaattt
    36961 tgtattcatt ctaattttgt tattcatcca atttcccatt tactcactag agaaaattaa
    37021 ccttgcagaa gattgtgaaa gaaatggatc tacagaatct tgccactctc atccatggta
    37081 tgtaattaat cagttttcgg aagaatttat aaattcagag aaaataagcg gcaactggaa
    37141 attagccgat aaattcccac taactccaga atcgtattat cctgatacag cagatacgtt
    37201 atttacaatg agaacacagt ttgatgctcc gaccgagtat ctaacaaatc ttagttttcc
    37261 agctctctat tttatttcac taggtgaagt ttattctatt tatttgaatg gaacccttgt
    37321 ttatcaagat gggaaatctg aaaatggaaa aatcttaatt agccgttatg ggaaaaatat
    37381 cctagttcct cttagcagta aactctttaa agaaaaggga aatcttttag tgataaaagt
    37441 agaaggaaca agaggatttg gtgccactgg cgtctatact gggaatagcg tatacataac
    37501 tgattataga gacgctgcgg cagttgcaag agatcgaatt ggtcttatat tgatttttct
    37561 ctatttattt gtaggtgctt atcatttatt tctctatttt aaaagaagag aagaagttta
    37621 taacctttat tttaccctgc tttcaattac aacatttctt tatatgttaa atagaatcgg
    37681 attttttcat gaacaaggat tcgactcggt tttgggttat agaatggaac ttctttctgc
    37741 ttatggaatc ttccctttta ttgtggcatt tatggaatcc ttattctttg gaaagcttag
    37801 taaattatta aaaggaattt ctttatctta cgcaatcatt ggaatcgcaa ctttattctc
    37861 tcctcctcct attctaaatc aattattact tctctggcaa ctatcggttt tacttggaat
    37921 tgtttataca ttttatattt tctataaagc aatcaaagaa aaaagcgaca atgctaagag
    37981 actctttttt gggtttttaa tcttaattgt attctcagta tttgacattc tagattcgat
    38041 tatttttaaa acaggttttc attttgcaag atatgcattt tttgcttttg taacaggaat
    38101 tgcagtcatt ttagcaaacc gttttttaaa tctctatgaa acaattgagg atttaaatca
    38161 aagtcttgag aagaaagtag aacttagaac gaaagaactt aggtatacac tcgaccaagt
    38221 aagactctta aaagaacaac aagatggaga ttatttttta accactctaa ttattcagcc
    38281 actagctacc aatacagcaa gtgatgatcg cttagaactt gatatactca tgagacaaaa
    38341 gaaaaaattt gagtttaaga aaagagtcta tgaaatcgga ggagatattt gtatttctga
    38401 tataataact ctcaaagaaa agaaatacac tgtatttatc aatggagatg ctatgggcaa
    38461 atccattcaa ggtgcaggag gagcgatagt tcttggcgtt gtatttaaat ctattatttc
    38521 tagaaccgtt atgtataaag gaaaccaaaa cgtataccca gaacgttggt taaaaatcgc
    38581 atttattgaa ttacaaaaaa tattcgaatc attcgatggt tctatgctgg tatctatcgt
    38641 aattggacta atggacaatg aatctggatt tttatatttt atcaatgctg aacatccttg
    38701 gagcgtttta tatagagacg agaaagcatc ttttcttgaa tcaggctttc atctcagaaa
    38761 ggttggaatg cttggagtag aaggctctct taaagtaaaa acatttaaaa tgcataaaaa
    38821 cgatattatt tttattggtt cagatggaag agatgatatt caattgggag aagatgaaac
    38881 aggtaggaga attatcaatg aaaatgaaaa tctattttta caaaccattg aagaagcgca
    38941 tggggattta gaaacaatac tttccataac agaaaagaaa ggagaaatca cagacgattt
    39001 cagtcttcta aaaattaaat acaaaggtga tgagcctatc tatctttcag atagaccaat
    39061 ttttattgag aatgcgatat ccgcaattca gaaggataaa ccaatagaag ctcttcaaat
    39121 cttagaacaa aataaaaacg aagaattggg tcaatatgcg aaagattatg cacaacttgt
    39181 actaaaagca gcaactctta gcacaagact acaagctggt tcaactctat taaatgaata
    39241 tatatggaaa tttcctgaag atgaaagtgc tatatatgga ctagctacac tgtattttaa
    39301 acaagggcag ttcgagaaag caaccgatct aggcgaaatt ctaaaactga gaaatccaga
    39361 tgactcaaaa gttttaaaac ttctagtaga aatctattct gcctcaggca atcacacccg
    39421 agccgaaaaa ttaaaactag aagtgagata acgttagttt gacttgaatt cgatctaata
    39481 acaagtttaa aatattacca ccgatataaa taaagaaatt taaaaacatc ggtgttcatc
    39541 ggtggtaaaa aattcttaca ccaaattttg attagcctaa atttagatca taactaaagc
    39601 cattctcttt gcagacttta ctataccagt ttgcactgag ttttggagtt cgttttaatg
    39661 tattacgatc gatatgcacc aagccaaatg ttttttcaaa accttcagcc cattcaaaat
    39721 tatccataaa agaccataca aaatatcctt tcacattgga tccttctaga attgctttgt
    39781 gcaaagaaga aagatattgt tgcaaaaaag aaattcgatt tttatcttct ataattccct
    39841 tttcatttgg tttttcaatg aaagcaactc cattttctgt gatcaataca ggaggattcc
    39901 cataatcttc tcgaatcatt ttcattaatt cataaagacc ctgaggataa atttcccaac
    39961 ccatacttgt aaattgaata cctttgtaga atggttttgc aggacgaaaa ttatagattg
    40021 gaaaagggaa ataacgcatc acaaaccttg tataattatt taccccaaga aaatctgttt
    40081 ttccagaaat cagtttcaaa tcatccggta aaatatttcc cttattttgc ttttcgataa
    40141 atggtaaaat ttcctctggg tatttacctt tataaattgg atccaaccag agtctgttta
    40201 ctatcgcatc ggctctacgg gtagattttg gttctgttgt gagtaaaaaa ttatgcacag
    40261 gagaaagagc atttgtaaga cccacttttg atttcgggta aattgatttg atacgagcaa
    40321 gtgcagtccc atgagctaac aatagattat gcgctacttt aaaagctgaa aagggtctaa
    40381 aaactccagg aggaaatact cccaagagat acccacatac aaatacaatc caaggttcat
    40441 ttagagtaat ccagtttgta actctatctc ctagtttttt taaaattact tcagtaaact
    40501 ctccaaaata ttcggcagta ttacgattgt accaacctcc ttctttttca agtgcaagag
    40561 gaagatccca atgaaataac gttataaaag gattaatttt cttttttaat aattcatcca
    40621 ctagtctgtc ataaaaatcg agtccctttg aattcacctg accctttcct tctggaagga
    40681 ctcttgtcca tgcaatagaa aaacgatagt tcttataacc aagcttactc ataagagcaa
    40741 tatcttcctt gtatcggtta taatgatcac aggtgatatc tccattttca ccatttttaa
    40801 ttttaccttt tttatgtgta aaatcatccc agacagaaag tccctttcca tcttgattgt
    40861 aagcaccttc aatttgataa gctgctgttg ccgttcccca gataaagtct tttggaaata
    40921 aaatcttttg cataaatcct ttatctagaa taattaggaa ttgaaaacaa aaaaagaaaa
    40981 tagattttca aggtggaaac tggctcaaat tattttgcat tacgacttag atatggaaat
    41041 attaacatac aaccaaggaa tataacgaac tgggaaatca gatagattta agaaaataaa
    41101 tttacatcct aaccatctgt gaatttcatc tctagatgaa ttcaaatcat tttgatctga
    41161 caggctctat ccttgtttgg ttggatgaac cgttatttga aagactttta aataatatta
    41221 acaggttttt cgaatggatg gtaagtaggg ttgtgtaaaa acttaaaaaa aatctaatca
    41281 tgaagagcac gaaggtcaaa atttcctagg aaatttaaaa tttttcttcg tgcacttcgt
    41341 ggttaaaatc ttgtttttac aaaattccta aattctagaa atttgctttt acacaaccct
    41401 ctttctctta tgttaagaga ttattttttt ccaagaccta ggcttggcgc acctactaga
    41461 atagacatat ttccatgtgg gtgtttgtta tttttcatga gttggtgaca agatcctgtt
    41521 tcctcgaatt taaatgttct agaaaggcat ggatcaattt taccagcgat cactaaatca
    41581 ttaaaacctt tgctgttttc atcatttgcg aaatgagaac cttgtaatct tttttgtctc
    41641 atccataaat agcgaagatc tacagtagcg ttaaatccag aagttccagc gcaaattaca
    41701 atcattccac ctgtctcaca aacaaaagca gaggttggaa ttgtagattc acctggatgc
    41761 tcaaatacga ttttaggatt tacaccttta ccccaaatgt cccaaagagc ttttccaaaa
    41821 tcacgcgctt ttttggtcca ttcagcaaat acttcaggtt tgttgatgtc actagtgatt
    41881 gctccccagt gtttgaaatt atttctattg attacaccct cagcgcctaa cttcttacaa
    41941 taatcaatct tatcatcaga actaacaaca gcgattggtc ttccgcctgc cgcttttaca
    42001 atttgaattg ccatagagcc aagtccacct gccccacccc aaattaaaac tggatctcct
    42061 ggttttacat cgtttggttt ccagttgtgt agcatacgat aggcggtagc ccctactaac
    42121 atataagcag cagattcttc ccaagataga tgttttggct ttggaagaca ttgatgatct
    42181 tgcactttac aaaattgagc gaatgatccc caatttgttt catagcccca aatgagttgt
    42241 gatggtgcaa acattggatc gttgcctgct tttacaaatg gatcattttt atcccataca
    42301 ccacagtgga ctacaacttc atctcccact ttcacatttt taacttcaga accaatttta
    42361 tatacaatac ctgatgcatc ggatccaccg atgtgaaatt tttctggctc tcctttttta
    42421 tttctagctc caattacatc caccggataa ccaagagccg cccaaacgtt attataattc
    42481 actccagcag ccataacagc aactagaact tcgtctggtt taatttcagg aacatcaatt
    42541 tgttctaact gaaatgcttt ttctggatct ccaaaacgct ctggacgaat gacctgcgca
    42601 tgcatttttt ttggcactac tccaactgga ggaagggttc ctactggaac gatttctggt
    42661 gtttcactca ttttaataaa tccttacttt gataagcaca ggttttattc tctatcttac
    42721 agggaaagta aaattttaaa cttgaacgca tggaatccat tctcaataca gaacaagcct
    42781 caagaatttg tagaaatgac tttactattt cataagtgtt tatcttccat ttttgttttt
    42841 aattgaatca atgttattca tactaagaaa ttaggacttg aacaaagatc agtaagaata
    42901 gaaacgcaat catttgattt tctttctatt cttactgtat tatagaatac tttccttttt
    42961 ttaggaaaat acttttgctt cttttaattt tgaatctagg aagtttaaaa agttagaagg
    43021 atatctaaca tcttgttttg aaacttcatc taacaacgcg atctcctctg tagataaatc
    43081 caatgcaagt gaatctatat tatcttgtaa ttgttccaat ttagaaacac caactaaaat
    43141 cgttgcacaa atcttttttg ctaatagcca agcaatagaa attgctgttg aggttgtatt
    43201 ctgctttttt gcaagctctg aaactttctc gactacagtg taggctttct ctcgatcaaa
    43261 taaattcaaa tcaaaggtat tgagtctacc gttttctggt tttggattgg atcttgtata
    43321 cttacctgtt agaaatccac tcgacaaagg actccaaatc ataagtccta gaccatgatg
    43381 agaagacatc ggaataaatt cctcttcgat ggaacgattg agtagagaat aatgcatttg
    43441 agctgaaacc aaaggagaat atcgtctctc tttttgtaat tgaaccatac tagctgtttt
    43501 ccaggcttgc caattactaa ctcccgcgta tcgtatttta ccctgcattt gtaaattctc
    43561 taaagccttt gcgatttctt ctagcggagt aattggatct tcagtgtgca gaaataaaat
    43621 gtcaaggtaa tcagtattta atctttttaa acttgcctca cattgctcaa tgatttgctt
    43681 ataactgatt cctgcattaa aaacttcttc tcctgttcta aatgctagtt tagaagatat
    43741 taaaacagat tttcttcttg atccgagcgc tttaccaagc atgatttcac tttgaccgtt
    43801 attatacata tttgctgtat caaaaagatt tatacccatc tcgatggatt tagaaattaa
    43861 agcgtctgct gttttttgat caattgaata ttttaatcca aagtaatccc cttctccacc
    43921 aaataacatt gtcccaatag atagtttaga tactacaagt cctgaatttc ctagttgtga
    43981 atatttcatt ttataactcc tttaaatttt actctacgta tttaacatta aacttaaaac
    44041 gatcaatgta ccatttttct tttttaacca aatgaatatc ataactacct acaaaagtct
    44101 taacgttctt tccatctttt acaggtaaat aatgagtagc cgttgcataa caaaaaacat
    44161 cggcagtatc gccatttatt tgaaccaagt aatttccagc ttggtgatgg attgctttta
    44221 gattttttag accattctcc catgcagagg taatttcagt atttgctaat aggttaggct
    44281 ttccgccagc aagagaagtc atatcaaagt caactcgatc tgcgaaaacg ctcttcacct
    44341 tttcccaatc tctattatct gtgaagatga aaagctcatt cactttttca attacatctt
    44401 gttttgcgtt taattcttgt aagtcccgat tcatgcaatt ctgcaataga gtgatgacta
    44461 gaattagagt taaaattatt tttgaaatct ttttcattta gaaatctcct tatctttttt
    44521 aaattataaa gagaaaaaga tttctttctt gtagattatt ggtttatttt aggaataaat
    44581 tgctattata ggattttatt ttctaatgca caattcgctt tctagaaatt ccatccgctt
    44641 ctattttctt aattctttca atgtttcgaa gagcatttgc aatttcattt tcatttccga
    44701 tgtttacgat ttcttcatat agatttttca ctcgtttgaa gtttcggttc tcttcttcta
    44761 aagctgtcat tctgaaaagt agatttgttt tctttaaact atcatagact atcttaattg
    44821 attttagtct aaaactagcc tcttctaatt tttcttgttc ttctttatac attgacagtg
    44881 attcagcagt agatttattt aataaatctc tctttaatag atcaacatct cgcttaacgt
    44941 tcgcaagact tgcatcttgg tcttttaata gagtcgatat ttctttatat gcagaatatg
    45001 ccctttctaa atagatttgc tcttcttctg gatattgcat ttgtttttta tagctagaaa
    45061 tggcatagga ggcaataaat tcagaagaaa gccgacgtgt tgtttcttct aaagagatgc
    45121 cgaactcttt atccgattct cttttttcct tttcttcttt tcttttttga tcgagccatg
    45181 ttaaccattt tgaaaaataa tcagagcctt tttcaaaatt tcccaatcga tgggaataaa
    45241 aatctcccaa ttgataaaaa aagtttggat cttccttttt gtcagagttt agaatttcta
    45301 acatcttttc atagtaattg gatgctaaaa catattgttt taaatctgtg taaagggctg
    45361 caacacttct gtagacatca taaagcggat acttaccatt attctctggg agggatgaca
    45421 attttatata tctcaaataa aaatctaggg catttctttt tttggcagaa gatttgtatt
    45481 catctgccaa aagtaaaatg gcttcagcat tgtttggatt tactttaatt gcaaactcta
    45541 gaagttgcga atacccaaca tagtcttgat ttcgattagt atctgaggca acaaaattcc
    45601 ctcttccaaa atgattcatc ttttcatcta ctttggattt ttctttagtt tcattttcaa
    45661 gttcttggat ttgttttgct agatcaacta tcgtcttgga atcagaagaa gttttatcat
    45721 ttacatattt tccaaaacga ccaagtaaag aatcggaata ttctttttct tttgctttaa
    45781 cctcatctct taaactatct attttagatc tattttccga aagctgtaca tttgcttccg
    45841 cctcacttaa atctcttttg gcatagagag acccgattcg tgcggaatta tcttcagctt
    45901 tttttaaatt ttgttttgct tcttctaaat catctttgat ttttttgtgt gccaatctct
    45961 gcgcagcttc ttcctcgctc aactcttcta ataaacttgg attgtagtat ctttcttccg
    46021 tattcgagta atctctatag cgaagagcgg aataatacga ttcgattgta ttatttctat
    46081 cgcctatctt ttcatagagt ttagcaaggc gtagatgtag gtcatagagt aaatcagaat
    46141 ctcgagctaa ctgagatggt gcatttttaa atttttgatt tgaaatgaat aatcgtatcg
    46201 aatttacatc cgcattgaca ggaatgactc cggagttatt tttaaattca tctgtcacat
    46261 tgtcttttct gtctttatat ctaccggctt tttcccttac atctagaatt cttttttctt
    46321 ctacaaattg cgcataatat tcatttaaca aagatttaat tttagaaatt tggtaggatg
    46381 gcttctccgc gttatccgct atatactttt ctaattcttc tcgtctttct tttgtaataa
    46441 cttcaattgg tttcttgtaa atttttttag agtcatctct ataatcatga gaatataaat
    46501 ttatagatag aattagagat agtatcaaag aaaaccgcat agttactata tcggtcaatt
    46561 tatttttttg attaagaaag gcttactcac cttcaggaag atgagttact tggtaacttc
    46621 tgctaattct tctgaggata aaatcttttc aatatccatg ataatgataa agcgattatc
    46681 cttttttgct acaccaatga tatatcgaga agacatccct ctcacggaag gaggaggagg
    46741 atctatctgt gctggtgaaa aatgaactac atgggaaacc ttatccacaa tcacgccaag
    46801 ggacttgcca tcaattttaa caataatggc tctattcata gaggattcgc ccactttttt
    46861 attcatgatc tttttactta aatcgatcat attaactact ttgcctcgaa tgtccataag
    46921 acccacaaag taattttgtg acttgggaac tttaatcaaa tttgtgactt ttacaatttc
    46981 ttctacattg ataatagata ttgcatagtc ttcttcacca attgtaaaag ttatatattg
    47041 ctcacttaaa tgatcatcat ctttcgccat ttatatcccc ttaaaatcta gtaaaccccg
    47101 tataaaaata atacccaccg aatgataaga aactcataac cttcaagtag aacggagttc
    47161 gatttcgcaa gatccaatct aaaccaccaa aaaaaacacc catgaatgca agcgaaaatc
    47221 ccaaaacaac ataatcaaaa aaatgtagat aaattgcaag acaaactgaa actcctaaga
    47281 tagaatcata caaatcatct aggaatctaa ttttaaaatc tgcatacgaa tcttttgctc
    47341 tgtgcaaaag tcttttataa aaagactctc ctgatacctg agtataaggc tctaactcac
    47401 ctgcatcact aggaatttct tcatgagctt ttttacctag agaaacttta gaaatatcat
    47461 aaacaccacc gatggaattt aaaacatcgt catcaggaat gcaggaaaat acattcttga
    47521 atggatgaaa ttttttcttg agggatgtat tcgttgcgtt aaatacatct ctctctcgaa
    47581 tgattgttcc attttttatg gtagctgtat aggttctgcc tttctcagaa gcaggttcta
    47641 aactaagacg aactaaattt tcatgaaaat tataatctac aacaaaaaca tctccgatag
    47701 gagatacagc tctataggat ttgaaatatc ctcttgcagg ggttttactg cgtttccacc
    47761 aagttctaaa accttcgtaa tccgaatcgt atagcatacg ttatctatcg gacacaaaaa
    47821 aaaaatctta agttaagatt caaggaggag tgtaaaaaat cgtattcctt tacaccctcc
    47881 ttttaaaatc ttaaggacta tttccctttt ttcttgtgtc tgttggctct acgctttttc
    47941 tttctcttgt gagtagctat tttttttctt tttctttttt taccagacgg cattcataac
    48001 tccttgtata atgttaaaaa tctattccaa atacttttgt aaacttctat ttttctttaa
    48061 ctcagataat agaagtttta tatctttgat tccagtttta gaggatataa ataaaacatc
    48121 atcctcctct actacaatta aatcctctac tccaagaaag gtaactaact ctttcttagt
    48181 agaagaaata ttcccttttg cgtgaaaatg aaaagaagct tttcctgtgt ggtgattccc
    48241 ttgtgaatct cctttcaata ctctttcgag tgacaaccag gatcctacat catcccaatc
    48301 aaagcttgct tttaccattc gaatcataga gcttttctcc ataatcgctg tatcaattgc
    48361 ttctgaagga agtaacttaa aagccgcact caactcgcca aagtttgcaa atggaaactt
    48421 attttttaag ggagataaaa tatagggagc atgcttttca aattcgctta aaataaaatc
    48481 tgtcttccag acaaagattc ctggattcca gtaaaaattc ttttttttca aatatttaag
    48541 tgctacttta taatcaggct tctcgaaaaa gccctttacc tgcattccat atttagtggg
    48601 ttttcctgtt ccaatataac cataaccagt ctcaggtcta acaggtttaa ttccaagtaa
    48661 taccaaactt tcttctgcct cttgcatcgc tagttttaac gtttttgtaa attcgttgac
    48721 cggttcaata aaagcatctg cagataagac aatctgtgtt gggtttccaa atttttgttt
    48781 aaaatataag gaggctagtg ctacgattgg agctgtattt tttccctcag gctctaggat
    48841 gaaatttttt tcaggaaatc ctttctcttg ctgtaaaatg gattttttta aagcagcatt
    48901 ggttccaata aatatacgat tgattgttgt aagagttaaa gctctatcga tggtttctct
    48961 tagaagtgtt ttattggaat acactttctg caattgctta ggagtagaga c
//
LOCUS       DAOQOS010000033        48100 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_287_length_48100_cov_7.140639, whole genome shotgun sequence.
ACCESSION   DAOQOS010000033 DAOQOS010000000
VERSION     DAOQOS010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 48100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 48100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48100
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_287_length_48100_cov_7.140639"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..836)
                     /locus_tag="PLF66_09200"
     CDS             complement(<1..836)
                     /locus_tag="PLF66_09200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64256.1"
                     /translation="MKFNSILYPIIFVSLLLCQSVYSDNFFTGFFDTGITESALWKTL
                     KDPTLREKAIQEIKKDIHKYVNLIIRLYNKGLEKDDREMLIDLISYSKNQTGENFLKT
                     NVSNKASEDRVFSFRKYMQDYKVDPAFIKGQLDDKQLRLYSLAYIVDRGKKEDYEVFQ
                     KIIFNANAPNEERILALKAAMLWASEIAKKEIYLFILQKGNEIVVAPSIMIFENIKSD
                     EIREELFRYSKKGQSQITRSNACYALTYYETDQVIPYLIVGLSEEFLYEKLTGIDILT
                     GL"
     gene            complement(1101..2777)
                     /gene="ilvB"
                     /locus_tag="PLF66_09205"
     CDS             complement(1101..2777)
                     /gene="ilvB"
                     /locus_tag="PLF66_09205"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020771015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HMZ64257.1"
                     /translation="MLVSGADIIIRYLEEIGIDLIFGIPGGANLPLYDRLAKSSIQHI
                     LARHEQGAAFMAQGYARSTGRVAVCFASSGPGVTNLLTGIADAKMDSIPIVAITGQVP
                     LSLIGTDAFQEIDTYGLTLPITKQTYLVKSPEELFHILPEAFSIATSNRPGPVVIDVP
                     KNIQNEKTLSKNFSSQKIKKVQSDFDLTRLEEVAYVIESSSKPILYVGGGMIQSNASP
                     LLMELSEKQSIPIVSTLMGLSSIPFDNPFNLGMLGMHGAPYTNYLLEECDLLIGLGVR
                     FDDRATGKVEEFCKNAKIIHVDIDASEIGKIKNPNYSFVGDVREFLEKINPLISRKPR
                     NNWMDRVEECKNAYPLSKISLSKNNPYRIIEMIGESVNEDTIITTDVGQHQMWVSQCY
                     PFKKTRTWLTSGGLGTMGFGIPAAIGAAFANPEKCVICFTGDGSILMNIQELDVISEY
                     NLNIKIILLNNNHLGLVRQQQELFYKENFIASKYHKPADFVKISKAFGIESDSLEENQ
                     SDYSGILNRLNQKGAYLLNIPIVAEEKVLPMVPAGAANRDMILKEKISMT"
     gene            3101..5173
                     /locus_tag="PLF66_09210"
     CDS             3101..5173
                     /locus_tag="PLF66_09210"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HMZ64258.1"
                     /translation="MKFLISVILSIVITINLYADTIDLTDSNEIYNLNGQWKFIESNH
                     IDYSLPNIDDSHFRLISIPGLWRQQNIEDINTGWYRRKIFIAENWRGKKISIITPSIT
                     DAHELYLNGVKISGEGEIDFSGKCVVNDSRANIVHIPDSLIQVNDINTLAIRVCNFAG
                     FAGSSGNFLIGPKVKIKERFYRSFGWNAAIGLVFLFVAFYHMILFLGSKKDLSYFYFS
                     VACFFAFTLNTGYFRFTYWANSSYWFHFYFFNSGFGIMPMFLVFFVHSFFEYRISFFV
                     RFLNYIVCSAFVLFLFSGFSIDIFKIYAQYGQPVQLLIGVPYAISVSILIIRAINEKK
                     DRAKIIGIGLVILLLAFGNSLLIYFNFVNTINLLPEAFLVFVLSIAVALAAKFYSVNE
                     QIILMQRHHSDELERKIRERTKDLEEAKKTAEIETGIAWIAQRESQEEKEKTEKLLKE
                     IQKDMTTAQKIQQNILPNNLDRIKKLKFATLYKPVSSVGGDYYDVEEIKPGLVRAFIA
                     DATGHGVQAALITMAIKSEYESVKDLLNSPARILKYLNDNFSNKYKSLHIYFTAFIID
                     IDTIYDRITYSSAGHAPQILINENKLEILSHRGNIIGMVSDVEYDEEVKDFNSGRLYL
                     FTDGIAEEFNEAQDQFGDERVIDVLEKSKSESLQKSIDNLYSEMLNFVGNARIRDDIT
                     VVGIEYMG"
     gene            complement(5179..5913)
                     /locus_tag="PLF66_09215"
     CDS             complement(5179..5913)
                     /locus_tag="PLF66_09215"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HMZ64259.1"
                     /translation="MKNCKILFLIFTFVTCIENNGNRFSEKFKYYYPNEEKFQSYIQE
                     VSKKNNIDWMFAKAVIIKESHYADHLVSQSGAVGLMQLMPRNGSYTTNAFVNYMIARK
                     QKRDKDGKRIYREKTVEEWGRLYQIELQTLYATNRERPNELYKLDKRFDPEWNIKEGI
                     RQLAAEYHFFLSRKHTEYESMILASAAYNAGRFAVMKSERNPKFDSIPINRETEKYIG
                     SMLRVFEALKKGEGFIPENKSWVLWL"
     gene            complement(5932..7854)
                     /gene="mnmC"
                     /locus_tag="PLF66_09220"
     CDS             complement(5932..7854)
                     /gene="mnmC"
                     /locus_tag="PLF66_09220"
                     /inference="COORDINATES: protein motif:HMM:NF002481.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)-methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016645 - oxidoreductase activity, acting
                     on the CH-NH group of donors [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HMZ64260.1"
                     /translation="MESAFSWQNTETLYSNIFQDIYWSNQGGPNEKEHVFLHGNNIVD
                     RLSLKKKFSIFEFGFGSGLNFFILRKQIRKMRITSRICYYSIEKYPLPVEIIQTILKK
                     LDHSDFDQNDFISFYSKLSSGFNSIYFPAENLELVLIINDVIDILPTFHAKIDAWYLD
                     GFSPSKNPEMWTKEIFSFIKRNSKQDATFSTYTTAKIVREGLSEVGFNIEKSVGFGQK
                     KEMLKGQIPISQQKIKDKPWYRSSNFKSNSKNVIIIGGGLAGTALAFALSREGYHSTI
                     LEKGESIACGASGNPAGMFAPILTGDDSELMDWSLHAFFKFVKFLKEIVGDSSEFYKQ
                     VGVVQKLSGEVEYNLYKTIVERLTSLQDLTDLYNQDDEYFIRFKDAGWINPGKLCETY
                     IELAREYCNVKLYAQVSKIESIESGWKVFLSSGDTLSSEIVVLANSFSIQELLPWFHF
                     YNKVRGQIVFYPSANIAFNLKNVFLHKDGYMIPNIKDYNVIGSTFDPNNSSSDLSIED
                     NLHLLQKLKNHFSEIDIRMAEKINGRVGFRAMTTDHLPIIGPIPDLDYFSEVYSDLWK
                     SNIYKDYPNGKYLSGLYLNCGHGSRGILNTFLAAHIITSMITGNPSNIKQELLNRIHP
                     ARFLIQSFIHRESFPK"
     gene            complement(7854..8162)
                     /locus_tag="PLF66_09225"
     CDS             complement(7854..8162)
                     /locus_tag="PLF66_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004420353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HMZ64261.1"
                     /translation="MASPVTRTEVNIFGSTYSISGETDPEYVRRLASFIDNKMRELSA
                     SLPSASVQKLAILAALNIADEFFQFKELNTEDDISRLYEMKTKKLISMLDEGLIGDIY
                     "
     gene            complement(8173..8892)
                     /locus_tag="PLF66_09230"
     CDS             complement(8173..8892)
                     /locus_tag="PLF66_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016543693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapB"
                     /protein_id="HMZ64262.1"
                     /translation="MISLETIEELETKIMKALELISDLRAENNRLESDNETLRSENDQ
                     MKLAMEEKEREVASLREQLAQSLKELEEFKSKEKKLDNKINDMISRLDNLSSFKTIDS
                     SSRPSSSAVVQSASTPEPTPAATPVTNNRVEEREPSDFEEDNDEIILLDDDEDEISEA
                     TNIVVEKAPEVSPEPVAEIKEEIAQPAVESSPISNPPVDASPILDDSDDIIIDETDDD
                     SINVFDSDDDDDFLIIEEEVK"
     gene            complement(8964..9317)
                     /gene="rplT"
                     /locus_tag="PLF66_09235"
     CDS             complement(8964..9317)
                     /gene="rplT"
                     /locus_tag="PLF66_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008595819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HMZ64263.1"
                     /translation="MSRAKNGTIHKNRRVKVLKAAKGFRGGRSKLFRTAKSAVMKAGQ
                     WAYRDRRAKKRDFRSLWIARINAAARENGISYSKFMSSLKKLGIDMNRKTLADIAYND
                     KEVFAAIVEKTKLAA"
     gene            complement(9317..9520)
                     /gene="rpmI"
                     /locus_tag="PLF66_09240"
     CDS             complement(9317..9520)
                     /gene="rpmI"
                     /locus_tag="PLF66_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010514334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HMZ64264.1"
                     /translation="MPKLKTNRAAAKRFKFTKNGKIKRASSYARHKLSGKSPKRKRKL
                     GGTTVLPESDMGRVIKLLPYGGK"
     gene            complement(9550..10083)
                     /gene="infC"
                     /locus_tag="PLF66_09245"
     CDS             complement(9550..10083)
                     /gene="infC"
                     /locus_tag="PLF66_09245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003001187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HMZ64265.1"
                     /translation="MHKKPNIRTNDKINTPRINEQITGPNVRLVSDTGSEVVTLENAL
                     KKAAEENLDLVEVSIAQDIPVVKIIDYGKFRFELLKKSREAKKKQHVVTIKEVKLRPR
                     IGVHDYEIKKKQAIEFLHKGDKVKVTLRFKGREMAHADLGMNVIVKIIEDLKDHGTPE
                     KSPVHDGKQIVVLINPK"
     gene            complement(10807..12276)
                     /locus_tag="PLF66_09250"
     CDS             complement(10807..12276)
                     /locus_tag="PLF66_09250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64266.1"
                     /translation="MKSLFYFLILFIGYFSIEAQSTPTHSIRGMYGDRAAGMGGAFAA
                     VSDDPSGAFYNPAGLGFISNTSVTTSVISYNDDRQSYNNFPSYGLSLQGKSMGITSGF
                     IGSVRSFGNFKVGFTIASPTNYNVDQYSNSFNSLGNTTNSVKLRLSESLSTTYVGPSF
                     AYMINEKISIGTTFFGFFERYRSSQEILVTASDNTYVNPSTESLRDSRGFLPIIGIQF
                     MPTSKFSLGLSIRKPFTTSGYERDFTRLTANNTNASNINFEDSSSSRYGAVIGNPSSL
                     GVPNNQTLFLGGNSQVNGRVPEPKEIRFGFAWFASKRFLLSADVIYTSGYHFNKYLYN
                     YEPTKRIFALTDNRIRALDLEPVTNFAAGLEYYLFEFLAIRLGYYTNYANAKNISWFE
                     SALKTSISSAAQFVTQNNGNTIVVALNDFRPSIDRNEFVNLRGITLGFSYETSKAIFS
                     ITYINESGRGNAQPVETILPAAYRVYNDRVYIGATIKQD"
     gene            complement(12323..13567)
                     /locus_tag="PLF66_09255"
     CDS             complement(12323..13567)
                     /locus_tag="PLF66_09255"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HMZ64267.1"
                     /translation="MIRLWNKIINIGIDENIDPLRKKSIQLTNGICLITIFMGMNFYP
                     NVTRFLPETLTLFIILTFALLFYIPTFIFSYYRFHLIAKIHLLIMASINITTPAILLG
                     KGQNFHFYLLTVIGISYFIFEAKEKIYQYSIIIYVSILFVATDLWLMTHKAAMSFPTE
                     FLYFAELNNDLGLLIFILGFNFYIYYNYTKAELALAEEKKKVEEEKAKSESLLRNILP
                     DPIALRLKENNFPIADGFNSVTILFADIVGFTPLSEKVSPDDLVILLNTIFSEFDYLT
                     KKYNIEKIKTIGDAYMVACGLPIPDTEHAVKIANFALEIRNLIQKLNQTHNRDLSIRI
                     GIHSGPAVAGVIGITKFTYDVWGNTVNTASRMESHSLPNKIQVSEATYQLLKPHFEMH
                     ERGMIEIKGKDPMKTYLLEGRI"
     gene            complement(13662..15665)
                     /locus_tag="PLF66_09260"
     CDS             complement(13662..15665)
                     /locus_tag="PLF66_09260"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HMZ64268.1"
                     /translation="MRLLIVFLSFFLFFLTQLSANEPILLNDQSDILHIDRGVYYLID
                     KDGKQSIDTIVNEKNLLVNQKNTLNFGYSSDPVWIKLPIRNESLNETRWLLEIAFPPL
                     DFIEIYIPSGDKYIQKKAGDRYPFWNRDFYYHHLLYSIDLPYGEEKNIYLRIQTTSSR
                     YVPIIIHKSSQFVKNVMPIEILHIIIFGCMFVMIFYNLFIYFGIRSNRYLLYSLGTFG
                     LLLYYFAVTGYGFQFIWPNSVFIQQYVIVFSVSWAGVFITEFTIQFLELKKRMPRIVI
                     IFRISQILAFLIFITVFISSRLANTVQSVVASLNSLLFLLVGIIVWVRGYRSARLFVI
                     GWCIFFAGIILNSMRNLGLFEIGLQSQFLMEIGTVFELIFLSLALADQYKLIVDESNL
                     MQKEILQMQIKYSESLEINVRQRTDELNTTLKSIRDDLLIAKQIQHNTLLIPYNLIDQ
                     IEIVPVYLTMSEVGGDFYSVDKLNEDTYRIFLADATGHGVQAALITMAIKGLYDNIKQ
                     FNAKPSVILELFNSEFVQRYKSLNTFCTTIILDIDTKNRKIKYASAGHSPCVLFQNKK
                     PILLEKTGKIIGITQNQRYSTIEYNFNLEDRLFLYTDGCFEEFNEMDEEFGEMKLLSS
                     LVNTLDLPLKDSIQNLLSEIRTFIGSKEMNDDITILGIEYKNN"
     gene            complement(16014..17930)
                     /gene="thrS"
                     /locus_tag="PLF66_09265"
     CDS             complement(16014..17930)
                     /gene="thrS"
                     /locus_tag="PLF66_09265"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HMZ64269.1"
                     /translation="MSQIQITLPDGSIREIEKGTTISDFVSKMSISLAKAALAAKVDG
                     LQVDLSYVLDKNTRLEILTYASKEGKEVFHHSSAHLLGMAVQRLWPDALLTVGPVVES
                     GPGFFYYDIDFPSVTITPEDLPKIEKEMEKIVKENLTVKRTVLTKEEAIQKFKEMGEK
                     YKMELISGFDSDTVTLYGQGEWFDLCRGPHVPNTGVLKAFKLTAISGAYWKANKDNAM
                     LQRIYGISFPSKKELDEYLFMVEEAKKRDHRKLGKELDLFSFQEEAPGFPFWHPKGTI
                     LWNNLANYIRSECTKRGYHEIKTPAILNSELWKQSGHWDNFKENMYFTEIDEQEFAVK
                     PMNCPGCSLIYKYHMHSYKELPIRYAELGTVHRHELSGVLHGLFRVRAFTQDDAHIYA
                     PLDHLESEVTDLIDFTFSVYKKFGFTEFKTYIATRPEKSQGRDEDWEEATSALIHALK
                     QKDIAFGIKEGEGAFYGPKIEFNIKDSLGRMWQCGTIQIDFSMPERFALDYTASDGKK
                     KRPVMIHRAIYGSLERFIGILIEHFEGKFPFWLSPNQIRILTVAEPHNAYAMELHHLF
                     LSKGIHSEVDTRSEKIGFKIRDSILKKANFLLILGDKEVESRSVSVRRLGEEKTTTYP
                     LDEFLALVEKERQV"
     gene            18394..18561
                     /locus_tag="PLF66_09270"
     CDS             18394..18561
                     /locus_tag="PLF66_09270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64270.1"
                     /translation="MPNRYRFQRESLSAYEDEIRWTGIAKFPYPIFIYLKRLSFGSQL
                     LAGHTGKNPND"
     gene            18903..19301
                     /locus_tag="PLF66_09275"
     CDS             18903..19301
                     /locus_tag="PLF66_09275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64271.1"
                     /translation="MNTIRVILLTSFLVTIFYGKPVEANCTYCYQIASVEITLRNKTI
                     QNGYISWSPNQPVNLNEKAFLEEAQKNQRIRFFKYLSKKNFGTEENEVSAYYALAKNV
                     ILLRRFDIKKLRIIDSNFGSSRSDIQIIKK"
     gene            19403..20656
                     /locus_tag="PLF66_09280"
     CDS             19403..20656
                     /locus_tag="PLF66_09280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64272.1"
                     /translation="MEKSLTYDKSINFVRSNSSTPYWYESVQNFDSGDYKKSFFNLLR
                     YVNSTITIPNESSESIELKIPHGSVIVSIKLDSKKYSIEAPFLKVPEGGSGIGMMRQI
                     AELNFSYLVLGQIVLRGNDFYIQYEDNLENCEPYKIYSLLEEICFCADYYDDVFAEKF
                     KTEYVVQPVLEVFSQAEKDLAYQKFNSIIKEGISFIDYFEMKRFYGLACDTLETTFLK
                     IDYVIAPQGILGSKLSETRGMLYANDSLQTVVANTKAKLQEFLSYDRAKFDSCLFHPK
                     FLIPIKKRGELPYIQDFMRNTYETMTGDMGNKSYMNVTVSALFLIYDLFYKNAIPQEI
                     TDHLDRALEKAGGKDWKTSAEALYDSVNKIMNLNPEEDITGKLGVTTNSNSSTGSSRE
                     IKSTVTKSIFGKITGLLKKELGDLL"
     gene            20653..21735
                     /locus_tag="PLF66_09285"
     CDS             20653..21735
                     /locus_tag="PLF66_09285"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HMZ64273.1"
                     /translation="MSDIDKIIPAIIKINTASGSGTGFYVNDYDLVITNYHVVSGYKK
                     VALEMQDKNTLAADVLVINPLIDIALLKPQKNLSTLPGVSFKRQDSIKNRDKVSVLGY
                     PFGMPFTITEGIISSTKQLLNGQAYIQTDAAVNPGNSGGPVVNMNGEIIGVTTSKFTQ
                     ADNMGFALPIDHVIDELEAYKLNPNIAYAVKCPSCNYALHEPMENCANCGAVLKKELF
                     NEQPKTEMAGFVEEVFKELNIDPVVARRGVDFWEFHRGSALVRYFVYRNSFLFATSPL
                     AKLPKTNLQEVYKYILTNPTSPFFLGIHEGVIYISYRTHLSDLKSSKRTQIQKNLGEL
                     ANKADELDNFLVETYKCEWTEESKKS"
     gene            21736..22458
                     /locus_tag="PLF66_09290"
     CDS             21736..22458
                     /locus_tag="PLF66_09290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64274.1"
                     /translation="MSAELHNFLVQTVKERIEPNKLMKLKKEFDGVLQALSPVKRFGP
                     RIYIGGVYEKRIDINTYADMDIHIYYPYDLQDHIREIAEYAFELVSKKFETRSLGLLY
                     RITISEDYDIDFVSGRAEDPEYGYSLLYDPETNQRQTGKLKEQLDLIRNARSMLKLVK
                     MWKRNKLLMWDSAKLEKSEMAYFLKRKDLNDYGIALQQWLLINMELKKKFAGQYEVQK
                     FIGACEESLKAMKDNFWIRTIF"
     gene            22481..23689
                     /locus_tag="PLF66_09295"
     CDS             22481..23689
                     /locus_tag="PLF66_09295"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HMZ64275.1"
                     /translation="MKLFGAFRFRNYRLWFIGQLASLIGTWMQATAQGYLIFQLTHSP
                     AYLGYVGFASGFPSLLTLVGGIASDRFSKKNVLLIAQVVMMILAIILSFLTYIEKIEA
                     WHIILLSFFLGIANAFDAPARQSLVSELVDQDHLTNAIALNSSMFNLARTIGPAIAGI
                     TYSFLGPAWCFLINALSFLAVILALLLIKTSKRENTKLSQKTVLNDFREGFIFAYENK
                     TVRILLLIIFISSIFGLGYVTLVPAWAVNILKGDASTVGSMQAIQGLGSFLGALFVAS
                     IGNIHYRGKLLVYALFSFPFILFIFSVIRFFPLVLVTLFIGGFGFMIVFNLANAIIQR
                     IVPNHLRGRVMSLYTFGFLGALPIGSLFMGTLAEYTSEPFSILVSSACMFIFVIWIWI
                     YTPYLRQSPN"
     gene            24277..26499
                     /locus_tag="PLF66_09300"
     CDS             24277..26499
                     /locus_tag="PLF66_09300"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HMZ64276.1"
                     /translation="MSYLSRLNNPPSSQSNESFAYPQNDYTLIANTTIIPIQPIITGN
                     LSNFKSTPPLPSGILIDPASGIISGTPAQIQVQTSYTISATGEKSEITSSISLTVQPN
                     GLLRQYTFTGGSYLDHNNSNPLTPNGSPINVIGSDGDSAGAILLNGSTDYLSNTSTNL
                     PLGSQPRTLCAWINPSNYSSLKMIFSYGSPGTISASIGLAFTQTNIGFVGWGNDFLPT
                     FQVPLNTWTHLCGTYDGTTATIYVNGSNIGSSPYTLNTVAGNFYIGNWVDLAGYFPGK
                     IDNVQIYNKSLNASEIRNLSIRVPAGLMAMFDFQGDTLDLSINKNNGTIMGGATLTID
                     RFGQANSAYLFNGSTQYINVPTYSGLPTGNTARTICAWFRSTTSNPENIVSFGTMTTT
                     NGNGIAKGGAAILFYGFADDTVGNHNNLTNIWVHSCGTYNGATATLYMNGAQIGSLSK
                     SWNTIISGFNIGRRLDNVEFFTGSIDDVRIYNRVLSINEIRSLSGYNPMQTSVWSSTP
                     ASSGLKLYFQSDSLSGLANNATVSSWTDQSGNLNTLNQGTGSKMPTFLTNGLNGRNTL
                     KFLSANMQELNNNATVGLGGTSFSIFAVAQPLSLGIDQGIIGISNSCGNDKVLGVDTT
                     NFLYGGICNLIYLTSSSFSITTIPNIFTETYQHGGGGNLGNLYQAGNLISSQVSSATP
                     FSGSTVLAIGRRYAAAAYWNGHISEVLFFDSALGNADRELVECYLSSKYGIGLGHSCP
                     "
     gene            complement(26510..26896)
                     /gene="cueR"
                     /locus_tag="PLF66_09305"
     CDS             complement(26510..26896)
                     /gene="cueR"
                     /locus_tag="PLF66_09305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010573822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006878 - cellular copper ion homeostasis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HMZ64277.1"
                     /translation="MNIGQLAKESGVSAKLIRHYEDIGLVPKVARTDKGYRIYLDSDV
                     HYLRFIKRARELGFSLPDIKKLVSLWKNKSRSSLQVKTLASKHLKELEKKLAQMKDMA
                     DTLRHLVHNCHGDSRPDCPILKKLED"
     gene            27072..27278
                     /locus_tag="PLF66_09310"
     CDS             27072..27278
                     /locus_tag="PLF66_09310"
                     /inference="COORDINATES: protein motif:HMM:NF012621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HMZ64278.1"
                     /translation="MTSTYQIEGMTCGHCVKQVEKEFSKAGINAKADLKSNSVSIDSE
                     LDSNAYSQIKTALEEEGYSLGNKI"
     gene            27292..29559
                     /locus_tag="PLF66_09315"
     CDS             27292..29559
                     /locus_tag="PLF66_09315"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020769091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HMZ64279.1"
                     /translation="MEDSKEITLDLFGMTCANCALRIEKGLRKMEGVEEARVNFARET
                     AFVKMKSSIQSEDLFSKVESLGYKASEHSDKNSEDAESKHKEEFNKLKKRFYISAILS
                     LPLLYTMVSHFTFLSFLPLPALLMHPWMQFLLAFPIQFWIGFPFYKGAYRALRNNAAN
                     MDVLVASGTSAAFGYSLVASITNGLRSGDILFLNALNHFDHIGHTAFPSLYYETSAVL
                     LTFILGGKWIETIARGKSSSAIKALLKLKPSTARVKKDSNWVEIPIEYIQVNDLLLVK
                     PGEKIPTDGIVIEGSSTVDESMLTGESLPVEKTVGNNLLGGTVNGNGAITLKVEKIGS
                     DTVLSSIIRTVEDAQATKAPLQQVADKIANIFVPVVFICAILDFILYFFWLDPNNFTG
                     ALEKSIAILVIACPCALGLATPVSLLVGTGKAAVKGILFRNADALEAIAQIQSIAFDK
                     TGTITEGKPYVVNFKTSTDTIDYPLQKIASAESGSEHPLAKAIVNLAKEKNLPLSKFE
                     NLKIEFNGGISASVETSKVYAGRKSYLENFNFKIPNDLVNIGIEWEKAGSTVFYGVVS
                     DTENHFMVFAIEDKVKETSKLAIEKIKSLGIEPILLTGDNDHISQKIAKEVGIQKVYS
                     SLRPEEKAEKIKQIQKEGKKIAMAGDGINDAPALASSDIGIAMGSGTDVAIETAGIVL
                     VKGDLLRLIDVIRISKQTVKNINQNFFWALCYNAIGIPIAGIGLLAPWLSGAAMAFSS
                     ISVVVNALSLNWKKD"
     gene            29564..29869
                     /locus_tag="PLF66_09320"
     CDS             29564..29869
                     /locus_tag="PLF66_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010577056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HMZ64280.1"
                     /translation="MKKIPNIHPGEILEEEFLMPLEITAYRLAKETAIPQTRISQIIK
                     GKRSITADTAIRFSKFFGTTPQFWLGLQNDYDQEGKRIQFNTKLQRNTGCLELKVIY"
     gene            29943..30437
                     /locus_tag="PLF66_09325"
     CDS             29943..30437
                     /locus_tag="PLF66_09325"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HMZ64281.1"
                     /translation="MLQIFIILLSILTFSTFANDFENETEDLLEACVKGNIKSARHHL
                     ENGASVNARANSGRTPGRTPIMWAAARGKKELVKLLIDHGAEVNAKDDWKGNMTKPDG
                     TEGWTALLFAADSDHEEVVKILIEKGADVNIKTKSGITALGLAHLWKNEEMIKMLKQA
                     GARE"
     gene            30438..30902
                     /locus_tag="PLF66_09330"
     CDS             30438..30902
                     /locus_tag="PLF66_09330"
                     /inference="COORDINATES: protein motif:HMM:NF017015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HMZ64282.1"
                     /translation="MLNQIQQLFKYNKVANLKYINALNNGIEYPDRAHELMIHILNAH
                     EIWLDRINPIQNKESVLPWDKRPGINKIDLNLTLYDRTDKFLKDNQIEIISRRNVKYQ
                     TTKGDLFENSIIDILHHIINHGSYHRGQISIFLREKDIAPPNSDYIMYVREL"
     gene            complement(30904..>31415)
                     /locus_tag="PLF66_09335"
     CDS             complement(30904..>31415)
                     /locus_tag="PLF66_09335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64283.1"
                     /translation="TNDKEDKNSNGKNGKDSKDKGNTGKDGKDSKEKGTNDKEDKNSN
                     GKNGKDSKDKGNTGKDGKENGNNGEDRNGQKDKGDNGKEGNDSKDGNELGEDDKDGGG
                     GGYLPADEGGPRKPVLTPDEQKRLGKEMERIQKRSGENKNHRRSRHQQDKTNKNQEEL
                     EQGLRDALW"
     assembly_gap    31416..31515
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<31516..32332)
                     /locus_tag="PLF66_09340"
     CDS             complement(<31516..32332)
                     /locus_tag="PLF66_09340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64284.1"
                     /translation="MNFSYSLFKKFVLLTFFLLFYTNCEFYKAYKYNEEGIQHLSESN
                     YEDSKTSFHKSISYSYKTYIPTYNIGNTLIKEDDFPSAHEKFQQAIEANPEFKEAIYN
                     DGQVLYLWGKKELAPETCDVERARVLFEQAMNRFKEVVDLPNAGDYKKAAGENSQYIE
                     SELTKLDELQENKCQQSQDQSKQDQNKSDSDQQDQDSENADQQLADNSGNPQDQKNQN
                     QKTDKTQDSKDSKNSNKKEGGDKNEKDSKNKEDKGKDSKEKGNTGKDGKESKDK"
     gene            complement(32843..34993)
                     /locus_tag="PLF66_09345"
     CDS             complement(32843..34993)
                     /locus_tag="PLF66_09345"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HMZ64285.1"
                     /translation="MKRFFLLTLFFAFYNIHPQTNEIPTLILNDLLEKYDLCDKDLPM
                     EMQVLKDRNRDLKFSTITKLNLPFQSCSDVPAKIRERALPYWFRFKVRNESKNFQEWI
                     LEIPYPLLDKIEIYSDQKIFWRDSPIYSGDNNFGLDKRNQKYTVSFQDGRDFEYKNFS
                     YNLHIPQNTEATIYIRVETEGAVIVPVVIRNHKAFIDYINNVNFAQGIYIGIILVMIF
                     YNLIIYFSIYKSIYIYYILSILSFSLWISSVNGLSFEWVFFKYPSIGNIFPLVTMPLY
                     VLFSGLFAKKFLSNEDTIQNFNKVFRLNYWFLLTIPLAPITSYYWLLQIQIAYTIGFC
                     FFLVWIAFFYKNQFKFGPANYYLTACIPLLICLTLHRFKVWGFANEAFWNSNELLQVC
                     SIWEAIFLSLAMREKLIRLRLDKEKADELLIEKQKELMNSYARFVPFELLGILNKSEI
                     TSVRLGDAIKKEMTVLFSDVRSFTSISEKMSPEENFEFLNDILRDISPVIRENNGFID
                     KYIGDAVMALFPIKPDDAIKSALSMLHSIQKFNSKRVEEGLLPIQIGIGINTGEMIFG
                     TIGEEQRMEATVISDAVNLASRVEGLTKHYGAHIIIAEQTFKKLTDVTHYNFRKLDIV
                     KVKGKEEKVTIYEILDNEESEVEKLKQETKEKFESALNLFFKNKFRDSKKLFKEILKT
                     NPEDKAAKMYLDRADLYIKEKIKLQDGFTEMKTK"
     gene            35156..35956
                     /locus_tag="PLF66_09350"
     CDS             35156..35956
                     /locus_tag="PLF66_09350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64286.1"
                     /translation="MKTTSKHYSFWTKIRDFQIDDTSAAFRFSDKLAKEQGWSKEYTD
                     RVIDEYKKFIFLICVTGQSCTPSEDVDQAWHMHLVYTKSYWIDFCRNTIGREIHHTPS
                     KGGSKETDNFKFQYEETKRLYENYFEEETPKDIWPDTESRFQFYFRRIDLREYVLIPK
                     PRIPKFARLGAFLSFGFLNSHCSGTGNGFGGFLLSISIVIGFIHLLGLIFTDSEDEKN
                     VRRRELNREAHNVHDLAQGESFSDGGIAGCGGDAGGCGGGCGGCGGCG"
     gene            35986..36621
                     /locus_tag="PLF66_09355"
     CDS             35986..36621
                     /locus_tag="PLF66_09355"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HMZ64287.1"
                     /translation="MKQIAIVENEEKYRNIVREELSSRTHWSLSFFTSAEEFLNDKNY
                     NRFDLIYVDINLIHMNGIDLVNTVWEKNPELKMVMLTGLNTDSYIFSALQAGAIGYLL
                     KTELSNLVYATDIFLEGGSFINPVIAFRIQTFFKRKKSNLKEELTQREIQIIEMIVQT
                     ESTERIADILKISINTVKNHIRNIYTKLQVNSRSNLVIKAMEIGIGYQKNK"
     gene            36618..38366
                     /locus_tag="PLF66_09360"
     CDS             36618..38366
                     /locus_tag="PLF66_09360"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HMZ64288.1"
                     /translation="MKILFYILVYFLILIFEIFPNDFSEKSHIFYLEDKNSEFTVKNL
                     GELPFNDSLEVSGFSNSTWWIKIDNIKTNSIVSIEYPLLDFVTFHYKDKQDGGWKKIE
                     TGDHFEFNSRQIRNKNFAFLFGDNIDLPLFVQIKTNGPVFIPIQIYSDLKYIEETNRE
                     SYLWGIYFGVIFIFLFYSFISFFSVKDSTYLFYMIHIVGFGMLQAVLNGIAFQFFWPN
                     SPSFANISPNIFHSISLLSALLFSMKFLRLKEYLSHGYKICIGLVLIYILIFLFSFNS
                     NLYKHLLIIQTYLIIASSFFIVSCSLLTIRSGYSPAKVHLIGWVFLFLAIILNRLKTL
                     GYLPHNIISSYGVLFASILEMGLFSIALGFRILKMHQEKESIEKERQKLISDLHDNLG
                     GDLVDIIYNLNKFANKSLIEKSDIETLKNTADKALYNLKTRISSKEESILLEDNFIDA
                     VRLLLISRYQNAKRNVNTSVSENAENFSNNNPLSVNELSDIYLICKEICTNDLKYGYG
                     VSIWNFDFENEYFSIQVLTNTNYTDKNKEGQGTLNLKKRGNKLGATIEVKTSESKFNL
                     IFKMKINSINFRSSKN"
     gene            38539..40113
                     /locus_tag="PLF66_09365"
     CDS             38539..40113
                     /locus_tag="PLF66_09365"
                     /inference="COORDINATES: protein motif:HMM:NF013132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008509 - monoatomic anion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006820 - monoatomic anion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HMZ64289.1"
                     /translation="MSLDKLEKEELKIWQKIGFFLGPFIFFSMLFFTPSSITETGSKG
                     SLTLEGWKVAACGIWMAIWWVTESVPIPITSLLPIPLFFLLNISNTVPKSVSEGLKEN
                     GVNKFFSEQILAPYADPIIYLFLGGFILAIGMEKWNLHKRIALNIIKIVGTKPSQIIL
                     GFLIATAFISMWASNASTTIMMLPIAITVLELLKSKLPSSEKHNIENFGVALMLSIAF
                     GASIGGLGTLIGTPPNAVMKAFFDKNGLDSINFSNWMIFGVSLIVISIPFTFILLTKI
                     IYPIKLAAIEGTDEIIETELKNLGKFSLAEALTALVIVATAMTWIFRGAYESNHLYIK
                     NNLKDGVIAMTGGISLFLIPVNWKEKKFLLEWSDMKAIPWDVLLLFGGGLSLAGAVDR
                     SGLSDWIGAQTKSLLEGETNIYLVVFVVTTMVMFLTEFTSNTATITLFLPIGLGIAKA
                     LSIDPRLILIPATIASSCAFMLPVGTPPNAIVFSSGYLKIPQMAKAGFWVNVFFAFLI
                     CILIFTLGVKVFKISS"
     gene            complement(40248..40745)
                     /locus_tag="PLF66_09370"
     CDS             complement(40248..40745)
                     /locus_tag="PLF66_09370"
                     /inference="COORDINATES: protein motif:HMM:NF019235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1564 family protein"
                     /protein_id="HMZ64290.1"
                     /translation="MILFENKSYRLDKQQTQFDKTTSSLLVPEHLIGILKRKIKENGS
                     LKLYFKSLLFRYRSLNFSGILPIPRKVKTEYQNKGLDLIRISFRVDNCDWIELGSLAL
                     ACGKSRCYLFVFLLELDTFGLTEIQSITDLNNVVATEANLSVESSWFLKRSTFLFERS
                     FHIRV"
     gene            complement(41072..42184)
                     /locus_tag="PLF66_09375"
     CDS             complement(41072..42184)
                     /locus_tag="PLF66_09375"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HMZ64291.1"
                     /translation="MIDFENILQFFWVLPWIAFIIYLYRKQRIAFEWVDNNVSKRFSS
                     VFTEYKNLNHLKLHILIIFAMGLLLIIAVAGPRMDGMVEDSSGKGKVILLLDGSFSML
                     SNDTSPHPVTKKYPEDRFAEAQIFSEELIDANPDYAYGLITFSGKPAVHSLPINDIIT
                     IKTFVHTLLVHNFESTGSSFKSALQEVLKIVYESEGNIQVILLSDGEVPEDKDENLEE
                     ELQLLKRNNVVVHTIGVGTEKGGGVNFFVSYTEEEKKEMMEKNRTDDSTGTKRKEVDQ
                     KIIKNIKTHRSDKILKQIAEATNGQYLVVEKGKWVEDLNPYLKETSAFSRSKIKTSGK
                     IDLSIYFLLAFFLIFVIHNFPKMSLIKNHISFYKKN"
     gene            complement(42181..43089)
                     /locus_tag="PLF66_09380"
     CDS             complement(42181..43089)
                     /locus_tag="PLF66_09380"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HMZ64292.1"
                     /translation="MSSYAPLQYKTPHNKRINYLYASYLVEGLILISLIIGLSDPHTV
                     TERNLIQEDGIDIALVLDVSASMQAADFKPNRLDAMKEIAKDFVKRSGGNRIGVYIFA
                     QDTFTQTPLTTDHSVLIELIESISFKVIDHSESGGTAIGDALLATTDGLQKAKKPKRD
                     QAIILITDGESSYGVDPILAAKYVLEQQIKLYVIGMAGEKPIEVYVEGRPYLTPSGKI
                     LVTSLDDKQLREIAKTANGKFYRAKNENVLAEIFSELGTLSRTPLEIKKSRLKHSYSR
                     YFAFAGLQFFFLWMFVYGLLIRRPMR"
     gene            complement(43185..43718)
                     /locus_tag="PLF66_09385"
     CDS             complement(43185..43718)
                     /locus_tag="PLF66_09385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64293.1"
                     /translation="MIFLLADLPNGIAPPVHILDLRYNQDVRNWILLPILLITLYWLY
                     RYFKSMQREIQKRKPKFPPEPIEVLKQKIETIHNQYSENRRSGLHELSSTMKKHFESK
                     MGIDIEEMTSEEISRRVKDKNITNFFLKLALLQFGKQHPEKEDFDEIFYEAKIASGIR
                     KTKQNEKPKSNEIVRPS"
     gene            complement(43715..44596)
                     /locus_tag="PLF66_09390"
     CDS             complement(43715..44596)
                     /locus_tag="PLF66_09390"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HMZ64294.1"
                     /translation="MLVSELLKKAKEIELIARKNAHSLLAGDYTSIIPGRGLQFHEAR
                     KYVYGESVRMIDWNMTARLGEPYVKTFLEEREREVLIALDVSPSMMTGYQDRTKIEFA
                     IELAATLAFSSMQSRDKIGYIIFNNEALEIVRPRGGKSQLFRTIKAFLQKTEEMKGKE
                     ATSDIRSVLHAVQKFKSSHFILFIISDFLDRDLPDDLKFARVEHDINMMHIYDPLEYQ
                     FTKEVFFPAISPEIEGNRKNFGFVNPGELGNLEQMKEYLKNASIKYRMSVGSFSTTAD
                     IGKALKEFFHLKKRVIV"
     gene            complement(44598..45563)
                     /locus_tag="PLF66_09395"
     CDS             complement(44598..45563)
                     /locus_tag="PLF66_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002770550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HMZ64295.1"
                     /translation="MSKNAEAVAQIKKIKEEIHKGIVGQESLVDGLILGILADGHILI
                     EGVPGLAKTRAVNLFANICNVQFKRLQFTPDLLPADIVGTRIYNQSKASFEVNKGPIF
                     TNFVLADEINRAPAKVQSALLETMQEKQVTIGEETFPVPRPFFVFATMNPVEQEGTYS
                     LPEAQLDRFLMKLIVNYPTKKEEADVVKMVIQETKLPSVEKLLNPDDILNLQKICRGI
                     FIEDKIISYITDLIQATRDPKAYGLNLENVIQYGASPRASIALALVGRARALMFGRDS
                     VMPEDIKAIAHNVLRHRILPTYYAEAEGIKTEQIINEILSKVKVP"
     gene            complement(45619..47850)
                     /gene="selD"
                     /locus_tag="PLF66_09400"
     CDS             complement(45619..47850)
                     /gene="selD"
                     /locus_tag="PLF66_09400"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR03169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HMZ64296.1"
                     /translation="MKNLVLVGGGQSHAPVLRKLGMEKNDLRITLISANSIVPYSGML
                     PGYIAGKYKAEECLIDLRHLCNFSGARFVNANVVGLDLDKKHVLLENRPPIPYEILSM
                     NIGIVPNIGDISGREYITPIKPALPFLEKWISILKKIEKGDFKNGFRLAILGAGAGGI
                     ETALTARERIMKIIPDFEIHIFQRGREILNGHNQRVKDKVIKILNQKGIILHLNEEFR
                     KISNKGKYFECLSGKGNIYNLDFIIGAMSPKPADWILDSGLMKDENGFILVNDYLESI
                     SHPNVFAVGDIASMKNYSRPKAGVFAVRQSEPLFKNIINLSKGKKLIKYKPQKNFLTL
                     LNIGDGTAIASRGKYALGASEWIWSWKDSIDQDFMEKFQSLPYKMTDMTQSESQDSLV
                     CKGCDSKAGSEIIKYALDNIEKYQMTLEQNRKEKKKLKTLVGLKEREDSSVIEVSKKM
                     NLIQSVDFFQPIISDYYLAGQITANHCLNDLYSKGVNGHCAHAIINAEAGVEKSEEDV
                     FQILAGAISVFNREGVELLGGHTGLARNFEVGFICSGFSDTPKFYSKKNAKEGDAIVI
                     TKPIGTGLIFAADMQNKANPFFIDAAIQFMLQSNFHLVEVLRKYKIGACTDVTGFGLA
                     GHLYEIMKASNLKARINLNKIPLVRGVEEILKKHPEIQSNLYPSNWKSFSPYISFTEE
                     SIVYRILFDPQTAGGFLFTLPESNVKNCLHDLESFGYTASIIGEIVKDRSPLEKFIEC
                     FLE"
     gene            complement(47855..>48100)
                     /locus_tag="PLF66_09405"
     CDS             complement(47855..>48100)
                     /locus_tag="PLF66_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016545674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="HMZ64297.1"
                     /translation="YYEAKGSFYSGGFDWLVVLLLCFFAGFIGLHRFYTGNFIIGTFQ
                     AMTLGGFGIWSFIDLILIITGSYRDGSGKPLVKNKRG"
BASE COUNT        15924 a         8358 c         7541 g        16177 t
ORIGIN      
        1 atcaagccag ttaatatatc tattcctgtt aacttttcat ataaaaactc ttccgataaa
       61 cctacaatta aatatggaat tacttgatcc gtctcataat acgttagcgc ataacaagca
      121 ttactccttg taatttgaga ttgtcccttc tttgaatacc taaatagttc ttctctaatt
      181 tcatcagatt taatattttc gaaaatcatg atagacggag cgacaacaat ttcattccct
      241 ttctgcaaga taaataagta aatttccttt ttagcaattt cagaagccca aagcattgcc
      301 gctttcaaag caaggattct ttcttcattt ggagcatttg cattaaaaat aatcttctga
      361 aatacttcat agtcctcttt tttacctcgg tccactatat aggcaaggga ataaagcctt
      421 aactgtttat catccaattg accttttata aaagctgggt cgactttgta atcttgcata
      481 tattttctaa aactaaaaac tcgatcttca gaggctttat ttgaaacgtt agttttgaga
      541 aaattttcac ccgtttgatt cttggaatag ctgattaagt caatcagcat ttctctatcg
      601 tccttttcta atcctttatt atacagacga atgattagat ttacatactt atgaatgtct
      661 ttttttattt cttggattgc tttttctcga agtgttggat ctttcaaagt tttccataaa
      721 gccgattcag ttatccctgt atcaaaaaag cctgtaaaaa aattgtcaga gtatacactt
      781 tgacagagaa gcaaagaaac aaatattatg ggataaagaa tagagttaaa tttcatacga
      841 ttaagattta atgcattcag attaattcaa atatttttta caaatcaaat tgatacttca
      901 acaattctgg tttttatcta aaataattga gttttagaaa acgattcgtc caaatgtgtt
      961 tcgcactgat gattttaggt ttatgtgaat gaaatgcgaa gaaaaggaac tcttttataa
     1021 acgaacagca atacaatcta taaaaaaaag aaaacattcg aatcagtttc tcattccttt
     1081 tcttcttaca aaattcattt ctaagtcatc gaaatctttt cttttaaaat catatctcga
     1141 ttggcagcac cagcaggcac cattggtaaa actttttctt ctgctacaat tggaatgttt
     1201 aatagatatg ctcctttttg atttaatcga ttcaatattc cagaatagtc tgattgattc
     1261 tcttctaaac tatccgattc aattccaaaa gctttggaaa ttttaacaaa atcagcaggc
     1321 ttatgatatt tagaagctat gaaattttct ttgtagaaaa gttcttgttg ttgtcttact
     1381 aaacctaaat gattattatt taacagaata atctttatat ttaagttgta ttcagatata
     1441 acatccaact cctgaatatt cattagtatg gatccatctc ctgtaaagca aataacgcat
     1501 ttttctggat ttgcaaaggc agctccaatg gctgctggta tgccaaagcc catagttccc
     1561 aatccaccgg atgttagcca agttcttgtt tttttgaagg gataacactg agaaacccac
     1621 atctgatgct gccctacatc ggttgtgata attgtatcct cgtttactga ttcaccaatc
     1681 atttcaatga ttcgatatgg attattctta cttagagaga ttttacttaa aggatatgca
     1741 tttttacatt cctctactct atccatccaa ttgtttcttg gtttcctaga aatgagtgga
     1801 tttatttttt ctaaaaattc tcttacatca ccaacaaaag aatagtttgg atttttaatt
     1861 ttacctatct ccgatgcatc aatatccaca tgaataatct ttgcattctt acaaaattct
     1921 tctacttttc ctgttgctct atcatcaaac cgaaccccta atccaatcaa caaatcacat
     1981 tcttctagaa gataattcgt ataaggagca ccatgcatac cgagcatacc taagttaaat
     2041 ggattatcaa atggaataga gctcaagccc atcagtgtag aaacgatagg aatagattgt
     2101 ttttcagata gctccattaa caatggactt gcgtttgatt gaatcattcc tccaccaaca
     2161 tataaaattg gtttggaaga agattcaatt acataggcaa cttcttctaa tcgagttaag
     2221 tcaaagtctg actgaacttt ctttattttc tgactagaaa aattttttga aagtgttttt
     2281 tcattttgaa tgtttttggg aacgtctatc acaacaggac cgggtcggtt cgatgttgca
     2341 atactaaaag cctcaggtaa aatatgaaaa agttcttcag gactttttac taaatatgtt
     2401 tgcttcgtga ttggaagcgt tagtccatat gtatcaatct cttgaaaagc atccgttcct
     2461 attaaagaaa gaggaacttg tcctgtaatt gctacaatcg gaattgaatc catttttgca
     2521 tctgcaattc cagttaaaag atttgtaaca ccaggtccag aagaagcaaa gcaaacagca
     2581 actcttcctg ttgatcttgc atatccctgt gccataaaag ccgctccctg ttcatgtcta
     2641 gccaatatat gctgaataga actttttgct aatctatcat ataacggtag atttgctcct
     2701 ccagggattc caaagatgag atcaatccca atctcttcta aataacgaat aattatatca
     2761 gctcctgata ctaacatttt aaaaatctcc tttgcaaaga cccttaaaaa caaaaaagcc
     2821 cccaccagct ttcgccgatg gaggcttttt acttttaagt atgtaaaatt acactctacg
     2881 gcgaatgata tgaatattcc acgaccacca cgaccacaga ggtagtaact aagaatattg
     2941 aatttaaaac gccgagttgt ttcatatttt gtttgcaagt ccaatcctac tgatatttgt
     3001 cattttgcaa gcagtaaaaa atgaaggtta tttttcttga ccaaaataag tattctatat
     3061 acattttatc atcctttcta aatacaaggg aatttacgga atgaaatttc tcattagtgt
     3121 aattttatcg attgttataa cgataaatct atatgcagat actatagatt taactgattc
     3181 taatgagatt tataatttaa atgggcaatg gaaatttatt gaatccaatc atatcgatta
     3241 ttccctaccc aatatagatg attctcattt tagattaatc tctatcccag gactttggcg
     3301 tcagcagaac attgaagata taaatactgg atggtataga agaaaaatct tcattgccga
     3361 gaattggcgt ggcaaaaaaa tttctatcat aactccatcc ataacagatg cccatgaact
     3421 ctacctgaat ggagtaaaaa tttcaggtga aggtgaaata gattttagcg gcaaatgtgt
     3481 agtaaacgac agtagagcga atatcgttca tattccagac tctctgatcc aagtaaatga
     3541 tataaatact ttggctatcc gtgtttgcaa cttcgcaggt tttgctggat caagtggaaa
     3601 ttttttaatt ggaccaaaag taaaaatcaa ggagcgtttt tatagaagct tcggttggaa
     3661 tgctgcaatt ggtttggttt ttctatttgt agctttttat catatgattt tatttttggg
     3721 atctaaaaaa gatctaagtt atttttattt ttcagttgct tgcttctttg catttacact
     3781 gaatactggc tacttccgat ttacctattg ggcaaatagt tcttattggt ttcactttta
     3841 tttttttaat tccggctttg gaattatgcc aatgttttta gtttttttcg ttcactcgtt
     3901 ttttgaatat agaatttctt tctttgttag atttctgaat tacattgttt gtagtgcgtt
     3961 cgttctattt ttattttctg gattctcaat tgatattttt aaaatctatg cacaatatgg
     4021 tcaaccagtt cagctgctaa ttggtgtccc ttatgcaatc tctgtctcca ttctaattat
     4081 aagagccatt aatgaaaaaa aggatcgtgc taaaattatt gggatcggtc ttgtgatttt
     4141 attgctggct tttggaaata gtcttcttat ttattttaat tttgtaaata caatcaattt
     4201 gttgccagaa gcattcttgg tttttgtttt atccatcgca gttgcgttag cagctaaatt
     4261 ttacagcgtc aacgaacaga ttattctgat gcaaagacat cattctgatg aactggaaag
     4321 aaaaatcaga gaaagaacca aagatttaga agaggcaaaa aaaacagcgg aaattgaaac
     4381 aggtatagct tggattgcac aaagagaatc acaggaagag aaggaaaaaa cagaaaaact
     4441 tctaaaagaa atccaaaaag atatgactac agctcaaaaa atccaacaaa atattctacc
     4501 taataattta gataggatta agaaattaaa atttgctact ctatataaac cagtgtcaag
     4561 cgtgggtgga gattattacg atgtagaaga aattaagcca ggattagtga gagcttttat
     4621 cgcagacgca actggtcatg gagtgcaggc tgcgttaatc acaatggcaa ttaaaagtga
     4681 atatgaaagt gtaaaagact tattaaattc accagctcga atcttaaaat acctaaatga
     4741 taatttttct aacaaatata aatcgttgca catttatttt actgctttta taattgatat
     4801 agatactatc tatgatcgta taacgtattc ctcagctggt cacgcacctc aaattctaat
     4861 caatgaaaat aaattagaga ttctttctca cagaggcaat ataattggta tggtttcaga
     4921 tgttgaatac gatgaagagg ttaaagattt caactctggt agattgtatt tatttacaga
     4981 tgggatcgct gaggagttta acgaagcaca agatcagttt ggtgacgaaa gagtaattga
     5041 cgttctagaa aaaagcaaat cggaaagtct tcaaaaatcg attgataatc tatattctga
     5101 aatgttaaat tttgttggca acgcaagaat cagagatgac ataacggttg tcggtataga
     5161 atatatgggc taatccaatt atagccataa aacccaagat ttattttccg gaataaaccc
     5221 ttctcccttt tttaatgcct caaaaactct taacatcgat ccaatatatt tttcagtctc
     5281 tcgattaatg gggatagaat cgaatttcgg atttctttct gatttcatta cagcaaatcg
     5341 tcctgcatta taagcagcac ttgcgagtat catgctttca tattctgtat gctttctaga
     5401 taaaaagaaa tgatattctg ctgcgagttg tcgaattcct tccttgatat tccattcagg
     5461 gtcaaatcgc ttatctaatt tataaagttc attaggtctc tctcgattgg tcgcataaag
     5521 ggtctgaagc tcaatttggt ataatcgccc ccattcttct actgttttct ctcgataaat
     5581 tcttttacca tccttatctc ttttttgctt gcgagcaatc atataattca caaatgcgtt
     5641 agtggtatag gatccatttc gtggcattag ttgcataaga ccgacagcac ccgattgtga
     5701 aactaaatga tcagcataat gagattcttt gattataaca gcctttgcaa acatccaatc
     5761 aatattattt tttttggaaa cttcttgtat gtaagattga aatttttctt cattcggata
     5821 gtagtattta aatttttcac taaagcgatt tccattattc tctatacaag ttacaaatgt
     5881 aaatataagg aaaagaattt tgcaattttt catatttctg ccaatattat tttatttagg
     5941 aaaagattct ctatgtataa aggactgaat taaaaatcga gccgggtgta tcctatttaa
     6001 taattcttgt ttgatattag aaggattgcc tgtaatcata gatgtaataa tatgagccgc
     6061 aagaaatgta ttcaatatac ctcttgagcc atgaccacaa ttcaaatata gccctgaaag
     6121 atatttacca ttcgggtagt ctttataaat atttgatttc cataaatcag aatacacttc
     6181 actgaaataa tctaaatcag gaataggtcc aatgattggt aaatgatccg tcgtcatcgc
     6241 tctaaagcca actcttccat ttattttttc agccatgcgg atatctatct cagaaaaatg
     6301 attcttaagt ttttgcagta aatggagatt atcctctata ctaagatctg atgaggagtt
     6361 atttggatca aaagttgatc caataacatt ataatctttt atatttggaa tcatatatcc
     6421 atctttatga agaaagacat tcttaagatt gaatgcaata ttcgcggaag gataaaaaac
     6481 aatttgccct ctcaccttat tataaaaatg aaaccaagga agaagttctt gtatcgaaaa
     6541 agaatttgca agcacaacta tttcagagga aagcgtatct cctgaagata aaaatacttt
     6601 ccaacctgat tctatggatt caattttact gacttgagca tacaatttaa cattacaata
     6661 ttctctagct agttctatat aagtctcaca gagcttgcca ggatttatcc aacctgcatc
     6721 tttaaatcgt ataaaatatt catcatcttg attatataaa tcagttaaat cttgcaaact
     6781 agtcaatctt tcaactatcg ttttataaag attatattct acttctccac ttaatttttg
     6841 aacaactcct acttgtttat aaaactcaga tgaatcacct acaatttcct ttaaaaattt
     6901 tacaaatttg aaaaaagcat gaagactcca atccattagt tctgagtcat caccagttaa
     6961 aatcggtgca aacataccag ctggatttcc agaagcgcca catgcaatcg attctccttt
     7021 ttcaagaatt gtagaatgat atccttctct agaaagtgcg aaagctaaag ctgttcccgc
     7081 aagtcctcct ccgatgataa ttacattctt agaatttgat ttaaagtttg atgagcgata
     7141 ccaaggttta tcttttattt tctgctggct aatagggatt tgccccttaa gcatttcttt
     7201 tttttgaccg aacccgacag acttttctat attaaatcca acttcggaca aaccctctct
     7261 gacaattttt gcagtagtat aagtagaaaa agtagcatcc tgttttgaat ttctttttat
     7321 aaaggagaat atttcttttg tccacatctc tggatttttg gaaggagaaa atccatctaa
     7381 ataccatgca tctatttttg catgaaaagt aggaagaata tctataacgt catttatgat
     7441 taaaaccaat tctaaatttt ccgctggaaa ataaatagaa ttgaatccac ttgagagttt
     7501 tgaataaaaa gatataaaat cattttgatc aaaatcagaa tgatctagtt tttttagaat
     7561 agtttgaata atttctactg gtagaggata tttctcaata gaatagtagc agatacgcga
     7621 agttatccgc attttacgaa tttgttttct taaaataaaa aagtttagtc ctgaaccaaa
     7681 accaaattca aatatggaaa actttttctt taaacttaat ctatctacta tattatttcc
     7741 atgtaaaaag acatgttctt tttcgttagg tccaccttga tttgaccaat aaatatcttg
     7801 aaaaatgttg gagtaaagag tttccgtatt ttgccaagaa aaagcagatt cgattagtat
     7861 atatctccga tcagtccttc atctagcatc gaaatcaatt tcttagtttt catctcataa
     7921 agtcttgata tatcatcttc cgtattcaac tctttgaatt gaaaaaactc atctgctata
     7981 ttgagggcgg ctagtattgc gagtttctga acacttgcac tcggaagaga tgcagacagt
     8041 tcacgcatct tgttatctat aaagcttgct aatctacgca catactctgg atcagtttct
     8101 ccagagattg aataggttga tccaaagata tttacttctg ttcttgttac aggagaagcc
     8161 atattttggg gcttatttta cttcttcctc aataataagg aagtcgtcat cgtcatcaga
     8221 gtcaaataca ttgattgagt catcatctgt ttcatctatg atgatatcat ccgagtcatc
     8281 gagaatagga gaggcgtcta caggtggatt agatattgga gatgattcta ctgctggttg
     8341 cgcaatttct tctttgattt cagcgacagg ttctggtgaa acttcaggtg ccttttcaac
     8401 aacaatattc gttgcttctg atatttcatc ttcatcatca tctaataaaa tgatttcatc
     8461 gttatcttct tcaaagtcag aaggttctct ttcttccact ctattatttg ttactggagt
     8521 tgcagcaggt gtaggttctg gcgtagaagc ggattgcacc actgccgaag aactaggtct
     8581 actagatgaa tctatcgtct taaaagaaga tagattatct aatcgactaa tcatgtcatt
     8641 gattttatta tctaatttct tttctttgga tttaaattcc tctaattctt ttagggattg
     8701 agcaagctgt tcccgtagag aagcaacctc tcgctctttt tcttccatag ctaacttcat
     8761 ctgatcattt tctgatctaa gagtttcatt atcggactca agacgattgt tttcggctct
     8821 taagtctgat atcaactcaa gtgccttcat tatcttagtc tctagctctt cgattgtttc
     8881 gagtgaaatc atacggaatc tcctaaatta atttcggtca tatcacacta aaaagaaagt
     8941 atttgcaact ataaaaaaaa tttttacgct gcaagctttg ttttttctac gatagcagca
     9001 aagacttctt tgtcattgta ggcaatgtct gctagagttt ttctattcat gtctattccc
     9061 agttttttaa gagaactcat gaatttagag taagaaattc cattttctct tgcagcagca
     9121 ttaattcgtg caatccagag agatctgaaa tctcttttct tagctcttct atctctatat
     9181 gcccattgac cagctttcat tactgcactt tttgcagttc tgaatagttt agatcttcct
     9241 cctctaaacc cttttgcggc tttgaggact ttaactcttc ggtttttgtg aattgttccg
     9301 ttttttgctc tagacattat tttcctccgt atggtaataa cttgattact ctgcccatat
     9361 cggactcagg taatacagtt gttccaccga gtttacgttt tcttttcgga gattttcctg
     9421 aaagtttatg gcgagcatag ctgcttgctc tttttatttt tccatttttt gtaaatttga
     9481 acctttttgc agcggctcta tttgttttca actttggcat tcttgtttcc taaatataaa
     9541 attcctaatt tactttgggt taattaaaac cacaatttgt ttcccatcat gcacaggaga
     9601 cttttccgga gtcccatgat ctttcaaatc ttcaattatt tttacaatta cattcatacc
     9661 taaatctgca tgtgccattt ctctaccctt aaatcgtaaa gtgactttta ctttatcacc
     9721 cttatgaaga aattcaatgg cttgcttctt tttaatttca tagtcatgaa caccaatacg
     9781 aggacgaagc tttacttctt ttattgtaac aacatgctgt ttctttttag cttctctgct
     9841 tttctttaaa agctcaaatc taaatttacc ataatcgata atcttaacaa ctggaatatc
     9901 ttgggcaata gacacttcaa ctaaatctaa attttcctca gcagcctttt tcagagcatt
     9961 ctcaagtgtg acaacctcag aaccagtatc acttactaat ctaacattgg gtcctgtaat
    10021 ctgttcatta attcgaggtg tatttatttt gtcatttgtt ctaatattag gctttttatg
    10081 cattcattct ccggattgat taacatcatt ttaagaacag aaacaaattc aagaaatttt
    10141 ttgtcatgta ctcgattttg aattagaaac gttcacggaa tcagaaaaat ctgcagattt
    10201 taaatgatta tgttccattc taggtttttt atctatgtta aaaactgtag tattttgatt
    10261 tctacataaa taatacattg aattcgatgc tatcggatca acccaaacct acattttaaa
    10321 gaagagaggc atttttaaat aaaaagccta ggttttctat acttttaaaa ataaacgtta
    10381 ataagtttca attctcctat aaaaaggatt gctgaccagt aaataaagaa ttttctcttt
    10441 ataaccttta tagattcatt tcactaataa gaattgaaat ccaatcctta atcgaaaaat
    10501 gctttcttaa aataaaattc tttactcttt tgtgctctta gtgcagtccg gcaaatatct
    10561 attatccgca atttaatatt gcgaaaactt tttaaaaata accaactcac cttattataa
    10621 ttctaattta aattcagcat gcgatcagag taggaatttc tataaatatc tagttcattc
    10681 accttcagga gaaattttca tcttactgaa ttactccgat tgaataatag aatttctttt
    10741 atataatata tttttaaaat agagtaaatc atcaaaagtt tgttcgaaat atgcatatac
    10801 caaactctaa tcctgtttga ttgtagcccc aatataaact ctatcattat aaactcgata
    10861 ggcagcaggt aggattgtct caacaggctg agcatttccc ctaccgcttt catttatata
    10921 ggtaatacta aaaattgcct ttgatgtttc ataagaaaag cctaaagtaa tacctcttag
    10981 atttacaaac tcatttcgat caatggaagg tctaaaatca ttcaatgcga ccactatcgt
    11041 atttccatta ttttgagtta caaattgcgc tgcagaagag attgaagttt ttagtgcaga
    11101 ttcaaaccaa cttatatttt ttgcatttgc ataatttgta taatatccca aacgaatcgc
    11161 tagaaattca aaaagataat attctaaacc tgcagcaaaa tttgtaactg gttctaaatc
    11221 caaagcacga attctattgt cagttaatgc aaaaattctc tttgtcggtt catagttata
    11281 taaatattta ttaaaatgat agccactggt ataaatgacg tcagccgaaa gaagaaagcg
    11341 tttggaagca aaccatgcaa agccaaacct aatttcttta ggttccggaa cacgcccatt
    11401 tacttgtgaa ttgccaccta aaaataatgt ttgattgtta gggactccta gactagaagg
    11461 atttccaatc actgcgccat acctagaact tgagctatct tcaaaattaa tattacttgc
    11521 atttgtatta tttgcagtga ggcgagtaaa atctctctca taaccagaag ttgtaaacgg
    11581 tttacgaatc gatagaccta aagaaaattt agaggtaggc ataaattgaa ttccaatgat
    11641 aggcaaaaag ccacgagaat cacgtaaaga ttctgtgctt ggattcacat aagtattatc
    11701 acttgccgtt accaatatct cttgtgaaga cctatacctt tcaaaaaaac caaagaaagt
    11761 tgttccgatg gaaatttttt catttatcat ataggcaaaa cttggaccca cataggtagt
    11821 agaaagagat tcacttaatc ttaactttac tgaatttgta gtatttccaa gagagttgaa
    11881 tgaattgctg tattgatcaa cattataatt cgtcggactt gcaatggtaa aacctacttt
    11941 aaaattacca aaggatctaa cgcttcctat aaaaccggaa gtaataccca ttgatttacc
    12001 ttgtaaactt aatccataag atggaaaatt attataagac tgcctatcat cattataact
    12061 aataacagat gttgtcacac ttgtatttga tataaaaccc aatcccgctg gattgtagaa
    12121 ggcaccagag ggatcatcag atacagctgc aaaagctcca cccatcccag cagccctgtc
    12181 tccatacata ccacgtatgc tgtgtgtagg cgtagactgt gcttcaatag aaaaataacc
    12241 gataaataga attaaaaaat aaaaaagact tttcattgaa ttcatatcct tataaacaaa
    12301 agaaattgca aatttgaaaa aattaaattc taccttccag caaatacgtt ttcattggat
    12361 ccttcccctt tatttcaatc attcctctct catgcatttc aaaatgaggt tttaataatt
    12421 gatacgttgc ttccgatact tggattttat ttggtagact atgtgattcc atacgactag
    12481 cagtattgac agtatttccc caaacgtcat acgtaaattt agttatgcct ataacacctg
    12541 caacagctgg tcctgaatgt attcctatac gtatactcaa atctctatta tgagtttgat
    12601 tcagcttttg aattagattt ctaatttcta aagcaaaatt agcaattttt accgcatgct
    12661 ctgtatcagg tataggcaat ccacaagcaa ccatataagc atcacctatc gtttttattt
    12721 tttcaatatt gtattttttt gtgaggtaat caaattccga aaaaatagta ttcaaaagaa
    12781 ttactaaatc atctggacta actttctcgg aaagaggtgt aaatcctaca atgtctgcaa
    12841 ataatattgt aacactatta aatccatccg ctataggaaa attattttct tttaatcgca
    12901 atgcaattgg atcaggcaga atatttcgaa gaagagattc acttttggct ttctcttctt
    12961 ctaccttttt cttttcttct gctaaggcaa gttctgcctt tgtataattg taataaatat
    13021 aaaaattaaa accaagtata aatataagta atcctaggtc attattcaat tcagcaaaat
    13081 ataaaaactc agtaggaaaa gacatagctg ccttatgcgt cataagccat aaatctgtcg
    13141 ctacaaataa aatggaaacg taaatgataa tagagtattg ataaatcttt tccttagcct
    13201 caaaaataaa ataagaaatt ccaattacag tcaataagta gaaatgaaaa ttttgtcctt
    13261 tgcctaaaag tattgctggt gtagtaatat taatactagc cattatcaaa agatgaattt
    13321 ttgcaattaa gtgaaagcga tagtagctaa atataaaagt cggaatataa aacagcaaag
    13381 caaaagttaa tataataaat aaagttaaag tctcgggcaa aaaacgagtt acatttggat
    13441 aaaagttcat acccatgaaa attgttatta aacaaatccc atttgttaat tgaattgatt
    13501 tctttcgaag aggatcaata ttttcatcta taccaatatt gattatttta ttccagagcc
    13561 ttatcatttt tacaccctag atctattgga ttaagatata aagaataaag caagaatatt
    13621 acaaagatat ctctatcact aaatagaatt gaatttatac cttaattatt tttatattca
    13681 atcccaagaa ttgttatatc gtcattcatt tccttagaac caataaaggt tctaatttca
    13741 gaaagtaaat tttggattga atcttttaga ggcaaatcaa gagtattcac aagagaactt
    13801 aaaagtttca tttctccaaa ctcttcatcc atttcattaa actcttcaaa acatccatct
    13861 gtatatagaa acaatctatc ctctaaatta aaattatatt ctatagtgga atagcgttga
    13921 ttctgggtta ttccaattat ttttcctgtt ttttccaaaa ggattggttt tttattttga
    13981 aataacacac aaggagaatg tccagccgat gcatatttta tttttctatt ctttgtatct
    14041 atatcaagaa taatagttgt gcaaaatgta tttaatgact tgtatctttg gacaaattca
    14101 ctattaaaga gttctaaaat tacagagggc ttagcattaa attgctttat attatcatac
    14161 aatcccttaa tcgccattgt aatcaaagcg gcttgcacgc catgacccgt tgcatcggct
    14221 aaaaatatac gataggtatc ttcatttaat ttatcaacac tataaaaatc accaccaact
    14281 tctgacatgg taagatagac tggaactatt tcgatttgat cgattaaatt ataaggaatt
    14341 aaaagagtat tatgctgaat ttgttttgct attaataagt catcacgtat tgatttaaga
    14401 gtagtattta gttcatcggt tctctgccta acgtttattt ctaaagattc agaatattta
    14461 atttgcattt gtaaaatttc tttttgcata agattacttt catcaacaat taatttatat
    14521 tggtcagcta gtgcgagtga caaaaaaatc aattcaaata ctgttccaat ttccattaaa
    14581 aactgtgatt gaagacctat ttcaaataat cctaaatttc gcattgaatt caatatgatt
    14641 ccagcaaaga aaatgcacca acctattaca aacaatcgag cagatctata tcctctaacc
    14701 caaacaataa tcccaaccag taaaaataat aatgagttta gactagctac aacagattga
    14761 actgtatttg ccaaacgact agaaataaaa acagtgataa atattaaaaa agcaagtatt
    14821 tgcgatatac gaaatattat aacaattcga ggcattcttt ttttaagctc taaaaattgt
    14881 attgtaaatt ctgttataaa aactcctgcc caagaaacag agaaaacaat gacatactgt
    14941 tgaataaaga cactattcgg ccaaatgaac tgaaatccat aaccagtcac agcgaaataa
    15001 tacaaaagta atccgaatgt tcctaacgaa tagagcaaat atcgattact acgaattcca
    15061 aagtaaataa aaagattata gaaaatcatt acaaacatac aaccaaaaat gataatatgg
    15121 agtatttcaa taggcatgac gtttttaaca aactgactcg atttgtgaat tatgattgga
    15181 acataccttg aacttgtagt ttgaattctt aggtaaatat ttttttcttc tccgtaagga
    15241 agatcgatag agtaaagcaa gtgatgataa taaaaatctc tgttccagaa agggtatcta
    15301 tctcccgctt ttttctggat atatttatca cccgaaggaa tgtatatttc aataaaatcc
    15361 aagggaggaa aagcaatttc cagtagccac ctagtctcgt ttaaactttc atttcgaatc
    15421 ggtaatttta tccaaactgg atctgaagaa taaccaaagt tcaaagtatt tttttggttt
    15481 actaaaagat ttttttcatt cacaatcgtg tctatcgatt gctttccatc tttatctatt
    15541 aaataatata cgcctctatc aatatgtaga atatcagatt gatcatttaa aagaattggt
    15601 tcattggcac tcagttgtgt gagaaaaaat aaaaaaaacg atagaaatac aataagtaat
    15661 ctcataacgg tttatcattt accatctaga tataaattca taaaatgtaa aggttatgtt
    15721 tggcaaacaa cttgtgttat ttaaaaattt tagtaatata tctttcatag gatggagtct
    15781 aatttacttc gaaagcgcga atgaaggaat cgaaagcgat ggattagagt cggactttcc
    15841 aaactttgct ttttcgctct ccatcctact ggtctaaata cttacagttg ttgattttat
    15901 gatttcttcc gtatttaaaa atcgactgtt gttcttagaa atcctagctt aacagaatca
    15961 atatttctat gtctagctag aaattactaa gaacaatatt gcgaaaattg aacctaaact
    16021 tgtctttctt tttccactaa cgctaaaaat tcatccaatg gataggtagt tgttttttct
    16081 tcgcccaatc ggcgaacaga aacagatcta ctttcaactt ctttgtcgcc taatattaaa
    16141 agaaagtttg ccttctttaa aattgagtca cggattttaa atccaatttt ttcagacctt
    16201 gtatctacct ctgaatgaat tcctttggac aagaacagat gatgcagctc catagcataa
    16261 gcgttatgcg gttctgctac cgtcagaatt cgaatttgat ttggagaaag ccaaaatgga
    16321 aatttaccct cgaagtgctc aattagaata ccaataaagc gttcaagaga accatagata
    16381 gctcgatgaa tcataactgg acgttttttc tttccatcgc ttgcggtata atctaaagca
    16441 aatctctcag gcatagaaaa atcaatttga atcgttccac attgccacat acgacctaag
    16501 ctatctttga tattgaattc tatcttcgga ccgtaaaaag caccttctcc ttccttgatt
    16561 ccaaaagcta tatctttctg ctttaaggca tgaatcagag cactagtagc ttcttcccaa
    16621 tcttcatctc gcccttgtga tttttccgga cgtgtagcga tataggtttt aaattcagta
    16681 aaaccaaatt tcttatatac agaaaaagta aaatcaatga ggtcagtaac ctctgattct
    16741 aaatgatcga gtggtgcgta gatatgtgca tcatcttgag taaaagcacg cactctaaaa
    16801 agtccatgca agactccact caattcatgt ctatgaacag ttcctaattc cgcataacgg
    16861 attggaagtt ctttatacga atgcatatgg tatttataga tgagtgaaca gcctggacaa
    16921 ttcataggct tcactgcaaa ttcttgttca tcaatttcag tgaaatacat attctctttg
    16981 aaattatccc aatgcccgct ttgtttccaa agttcagaat ttaaaatcgc tggagtttta
    17041 atttcatgat aacctctttt cgtgcactca gagcgaatat aattcgcgag attattccaa
    17101 agaatcgttc ccttaggatg ccaaaaagga aatccgggag cttcctcttg aaatgaaaat
    17161 aaatctaatt cttttcctaa ttttctatgg tctctttttt tggcttcttc gaccataaac
    17221 aagtattcat ctaattcttt cttagatgga aatgatattc cataaattcg ctgaagcatt
    17281 gcattgtctt tatttgcttt ccaataagct ccagatattg cggttaactt aaatgctttt
    17341 aaaacgccag tatttggaac gtgaggacca cgacaaagat caaaccactc cccttgccca
    17401 taaagagtta ctgtatcaga atcaaaacca gaaataagtt ccattttata cttttcaccc
    17461 atttctttga atttttgaat agcctcttcc tttgttagga cagtgcgctt aacggttaga
    17521 ttttctttta caattttttc catttccttt tcgatctttg gtaaatcttc aggagtaata
    17581 gttacagatg gaaaatctat atcatagtaa aaaaatccag gacctgattc cacaacggga
    17641 cctacggtga gtaacgcatc tggccaaaga cgttgcactg ccattcctag taaatgagca
    17701 gaagaatgat gaaaaacttc ctttccttct tttgatgcat aggtaagtat ttctaggcga
    17761 gtattcttat ctaatacata agatagatct acttgcagtc catcaacctt tgcggctagg
    17821 gctgcctttg ctaaagaaat gctcatcttg gagacgaaat ccgaaatagt agttcccttt
    17881 tctatttctc gaatgcttcc atcgggtaaa gtgatttgta tctggctcat tcatccaaaa
    17941 tttcagaaaa tagactatcg gcaagtgatt ttcatacgaa tggcattatc tttgctatta
    18001 gccgcccaaa aaagcttaca ttcatgatta aaaaaagaaa ctcaattcat ggttcaagta
    18061 gatgtcattc aaagcctttc cgaatttcta tattacaaat tcaagtatcc aaatttttcc
    18121 gttgtgctgc aaggctttgc aataagtgaa tttataaaaa agaattccaa gtctagcttt
    18181 ctttctgaaa atgaaaaaaa tttcgtcatt gaatttggcg aattcctttc tatttccaaa
    18241 aatatatcca aaaaaacttt atccatcatt tgaagacaac tcctattcct tcgaaaaaaa
    18301 gatctgaatt gaaaaatgga atctgtgaat tcaaattaaa tctagaaata ggaataccta
    18361 aagaacaagt attaaaagaa attgaggctt tgattgccaa accgctatcg atttcaacga
    18421 gagtcacttt ctgcctatga ggatgaaatt cgttggactg gtattgcaaa atttccatat
    18481 cctattttta tttatttaaa gcgcctatcg tttggatctc agctattggc tgggcacaca
    18541 ggaaaaaatc ccaatgattg aagagattga gtatagatta gcttccatta aaaagtatat
    18601 atacgaaata aaaaatgaaa cttcttaaaa ggatttttac ttgagaagca tatagtatac
    18661 cgaacatgct ccagtgtgag aatactaaga aaagaagtat ttaaaccaaa cacttatttg
    18721 tgattcaaaa acgagaagtt aattttgatg ttcggcataa ttatagtaga acaaaaagat
    18781 tctagaacac tacttttcat gcaaaagaag gtgagactac aaagagaact cagttccgta
    18841 ttgtgatttc caagaatggc tcgctttaaa agatggaaat catattccaa atgaagatac
    18901 caatgaatac tattagagta atattactaa cgtctttttt agtaactatt ttttacggca
    18961 aaccagttga ggctaactgc acatactgct atcaaatcgc ttccgttgaa attacacttc
    19021 ggaataaaac aattcagaat gggtatattt cttggagtcc aaatcaacca gtaaatttaa
    19081 acgagaaagc attcctagaa gaagcacaaa aaaatcaaag aattcgcttt tttaaatatc
    19141 tttccaaaaa gaattttggc acggaagaaa atgaggttag tgcttattat gcgctcgcaa
    19201 agaatgtaat tctcttgcgt agatttgata taaaaaaact cagaatcata gactcaaatt
    19261 ttggttcctc tcgctcagat attcaaatta taaaaaaata aaatgtgttt tttataaaaa
    19321 aatcattcaa tttagaaaat cattgcattt ttaattgtaa aaaaaaatag ttaacactat
    19381 tgcatataca aaagaggctt acatggagaa atccctgacc tatgataaat caattaactt
    19441 cgtaagaagt aattcgtcca ctccgtattg gtatgaatcc gttcagaact tcgatagcgg
    19501 tgactataaa aagtcgtttt ttaatttact acgctatgta aacagcacca tcacaattcc
    19561 gaatgaatct tcggaatcaa ttgaattaaa aatacctcat ggttctgtaa tcgtttctat
    19621 aaagctagat tcaaaaaagt attcgataga agctcccttt ctaaaagttc ccgaaggcgg
    19681 gagcggaatt ggtatgatgc gtcaaattgc agaattaaat ttttcctact tagttctagg
    19741 acaaattgtt ttacgtggaa atgattttta tattcaatat gaagataatt tagaaaactg
    19801 cgaaccatac aaaatctatt ctctattaga agaaatttgc ttttgtgcag attattatga
    19861 tgatgttttt gctgaaaaat tcaaaactga atacgtagtc caaccagttt tagaagtatt
    19921 tagccaagca gaaaaagatc tagcctatca aaaattcaac tcaattatta aagaaggaat
    19981 ttcatttatt gactattttg aaatgaagcg cttctacggt ttagcttgtg acacactaga
    20041 aacaacgttt ttaaaaatag actatgtgat tgctcctcaa gggatcttag gttctaaatt
    20101 aagtgaaaca cgtggaatgc tttacgcaaa tgactctctt cagacggtag tcgcaaacac
    20161 aaaggctaaa ctgcaagaat ttctttccta tgatagagct aaatttgatt cttgcctttt
    20221 tcatcctaag tttttaattc caattaaaaa aagaggggag ttgccatata ttcaagattt
    20281 tatgagaaac acttatgaaa caatgacagg cgatatggga aataaatcgt atatgaatgt
    20341 aactgtttca gctttatttt taatctatga tttattctat aaaaatgcaa ttcctcaaga
    20401 aataacagac catttagata gagcattaga aaaagcaggc ggtaaggatt ggaagacaag
    20461 cgcagaagct ttgtatgact ctgtaaataa aattatgaat ttaaatccag aggaagatat
    20521 tacgggcaaa ttaggagtaa ccacaaacag taattcatcc acaggtagct ctcgtgaaat
    20581 aaaatcaaca gtcacaaaga gtatttttgg aaaaataacc ggcttactca aaaaagaatt
    20641 gggagatcta ttatgagcga tatcgataaa attatacctg ccattatcaa aatcaacaca
    20701 gcaagtggaa gtggaacagg tttctatgtg aatgactacg acttagtaat tacaaattac
    20761 cacgttgtaa gtggttacaa aaaagtagca ctagagatgc aggataaaaa cacccttgcc
    20821 gcggatgtgc ttgtcattaa ccctttgatc gatattgcac ttcttaaacc gcagaaaaat
    20881 ttaagcactc taccaggtgt tagctttaaa agacaagata gcataaaaaa tcgagataaa
    20941 gtatctgttc ttggttatcc atttggaatg cctttcacaa taacagaagg gatcatatca
    21001 tcgactaaac aacttctaaa tggtcaggct tacattcaaa cggatgcagc agtcaatccg
    21061 ggtaacagcg gcggacccgt agtaaatatg aatggggaaa ttattggagt tacaactagt
    21121 aaatttactc aagcggataa tatgggtttt gcgcttccaa ttgatcatgt catcgatgag
    21181 ttagaggctt acaaattaaa tccaaacatt gcgtatgcag taaaatgtcc ttcttgtaac
    21241 tatgctttac atgaaccaat ggaaaattgt gcaaattgtg gagctgttct taaaaaagaa
    21301 ttatttaacg aacaaccaaa aactgaaatg gctggatttg ttgaggaagt cttcaaagaa
    21361 ttaaacattg atcctgttgt cgcaagacgt ggagttgatt tctgggaatt tcacagaggt
    21421 agtgcgttag ttcgttactt tgtttataga aatagctttt tatttgcaac tagtccacta
    21481 gctaaacttc caaaaacaaa tctacaagaa gtttataaat atattttaac aaatcctaca
    21541 agcccattct ttctaggtat tcatgaagga gttatttata tttcctacag aacccatcta
    21601 tccgatttaa agtcttccaa gaggactcaa atccaaaaga atttaggcga actggcaaat
    21661 aaagcagatg agttagacaa tttcttagtc gaaacttata aatgcgaatg gacggaagaa
    21721 tctaaaaaaa gttaaatgtc agcagaactc cacaatttct tagttcaaac tgttaaagaa
    21781 agaattgagc caaataagct catgaaatta aaaaaagaat ttgatggagt tttacaggcg
    21841 ttatcccccg taaaaagatt cggaccccga atctacatcg ggggtgtcta tgaaaaacga
    21901 atagatataa atacttatgc agacatggat attcatatct attatcctta tgacctacaa
    21961 gatcatattc gagaaattgc ggaatatgct ttcgaacttg tctcaaagaa atttgaaact
    22021 cgttcattag gacttcttta tagaatcaca ataagcgaag actatgatat tgactttgta
    22081 tcaggaagag cggaagatcc tgaatacggt tactcattgt tatatgatcc agaaacaaat
    22141 caaagacaaa ctggcaaact aaaagaacaa ttagatctga ttcgaaatgc taggtctatg
    22201 ttaaaattag taaagatgtg gaaaagaaat aagttactta tgtgggattc tgcaaaacta
    22261 gagaaatcag aaatggcata ctttctaaaa cgaaaggact taaatgatta tggtatcgcc
    22321 ctgcaacaat ggctcctaat taatatggaa ttaaaaaaga aattcgcagg tcaatatgaa
    22381 gtgcaaaaat tcattggtgc ctgcgaggaa tcccttaaag caatgaaaga taatttttgg
    22441 attcgaacta tattctaaaa aagacatagg cttttacagt ttgaaattat ttggtgcatt
    22501 tcgcttcaga aactatagac tttggtttat aggtcaatta gcatccctga ttggaacatg
    22561 gatgcaagct actgcgcaag gatatttaat attccaatta acgcattctc ctgcttattt
    22621 aggttatgtt ggattcgcat ccggctttcc ctctctttta acgttagtcg gcgggatagc
    22681 atcggatcgt ttttcaaaga aaaatgtttt gctcattgca caagtggtaa tgatgattct
    22741 tgctataata ctttcctttt taacttatat tgaaaaaatt gaagcatggc atattattct
    22801 attgtcattt tttcttggga ttgcaaatgc atttgatgct ccagcgagac aatcattggt
    22861 ttccgaatta gtggaccagg atcatttaac aaatgcaatt gcattgaatt ccagtatgtt
    22921 taatttagct cgcacaatag gtcctgccat tgcaggtatt acatattctt ttttaggtcc
    22981 agcttggtgc tttttaatca atgccttatc ttttttagct gtgattctag ctttattatt
    23041 aatcaaaaca tcgaagcggg aaaatactaa attatcacaa aaaactgttt tgaatgattt
    23101 tagagagggg tttatttttg cttatgaaaa taaaaccgta agaattctac tacttattat
    23161 ttttatatca agtatattcg gtttgggtta tgtaactctt gttcctgctt gggcggtaaa
    23221 tattttaaag ggggatgcta gcacagttgg ttctatgcaa gcgattcagg gtttaggatc
    23281 ctttttagga gcgctatttg tagcaagtat tggaaatatt cattatagag ggaagttatt
    23341 agtatatgcg ttattctcct ttccatttat tcttttcatt ttttctgtta ttaggttttt
    23401 tccgctagta ttagttactt tatttattgg tggttttggg tttatgatag tttttaattt
    23461 agctaatgca atcattcaga gaattgttcc aaatcatctt agaggaagag ttatgagtct
    23521 atatactttt ggatttttgg gagctctacc aattggttct ctttttatgg gaacgttagc
    23581 cgaatacact agcgaaccgt tttccatttt agtttctagt gcttgtatgt ttatatttgt
    23641 tatctggatt tggatttaca ctccatatct aaggcaatca cctaattaat ttttcttttg
    23701 acaaaaattc attctaataa aaagccaaag aggaaacaat actagaatta gaataatcca
    23761 cctattttaa tactcaaact cttatttgtt aatcatccgt tatacaccaa ataacagcga
    23821 acaaaagtaa tttactataa gaatttccga gtagaatttt gcataccaaa cctactgcaa
    23881 cttagaagtg tttaaaccaa atacattttt ttatttttaa aataaaacgt tccttttgga
    23941 gttcgttgtt atagacaaag aataagattt gacattctag gaatttaata caaactaatt
    24001 tattcaatat gaactcaaaa caaggtttga gaaagttgtt agacgatgat tcctaaaatt
    24061 tcattttgga tttaaaatcc taaagatttc ctttttttta ggaaaaagat taaacatctc
    24121 tttatctagt tcctgttgat ttaaattttc aaaatttttt aagtctaaaa aaacaatgtc
    24181 gggtatatca aaataatgag actattatat tttttattat tatcatcgat cctattttgc
    24241 aaatctattg aggaggataa atcgaatttc tctttcttga gttatctctc tcgtttaaat
    24301 aatcctccct cttcacagtc gaatgaaagt tttgcttacc cccaaaatga ttatacatta
    24361 atcgcaaata caacaatcat cccaatccaa ccaatcataa caggcaatct ctcaaacttt
    24421 aaatctaccc ctcctttgcc atccggaatt ctaattgatc ctgctagtgg aatcatttct
    24481 ggaactcctg cacagataca ggttcagacg agttatacca tttctgcaac aggagaaaaa
    24541 agtgaaatca caagtagtat ctcactcaca gtgcaaccga atggtttact caggcagtat
    24601 acttttaccg gtggaagtta tctcgaccat aataactcga atcctttaac accgaatgga
    24661 tcaccgatca atgtaattgg atccgatgga gactcagctg gagcaatctt actaaacggg
    24721 tcaacagatt acctctctaa tacttccacc aatctaccgt taggctctca accacgaaca
    24781 ctttgtgctt ggatcaatcc gtcaaactac tcctctttaa aaatgatttt ttcttacgga
    24841 agtcctggaa caataagcgc atctattgga cttgcattta cacaaaccaa tataggtttt
    24901 gttggttggg gaaatgattt tttaccaacc tttcaagttc ctttaaatac ctggacgcat
    24961 ctctgtggaa cttacgatgg aactactgca acgatatacg tcaatggcag taatattgga
    25021 agttcaccgt atactttaaa tacggtagct ggcaatttct atattggaaa ttgggtagac
    25081 ttagccgggt attttccagg taaaatagat aatgtgcaaa tatataataa atcgcttaac
    25141 gcttctgaaa taagaaattt atcaattcga gtccccgcag gtctaatggc tatgtttgat
    25201 tttcaagggg atactcttga tttgtcgatc aataaaaaca atggaactat aatgggagga
    25261 gcaacactca caatagacag atttggacag gcaaactctg cttacctttt taatggctca
    25321 actcagtata ttaacgttcc tacgtattct ggtctcccaa caggaaacac cgcgaggaca
    25381 atttgtgcat ggttcagatc tacaaccagt aatcccgaaa acattgtaag tttcggaacc
    25441 atgactacaa cgaatggaaa tgggattgcg aaaggaggag ccgcaatatt attttatgga
    25501 tttgctgatg atacggtcgg aaatcataat aatctaacaa atatttgggt tcactcttgt
    25561 ggaacttaca atggggcaac agcgactctt tacatgaacg gagcgcaaat aggatctcta
    25621 tccaaatctt ggaatacaat catcagtggt tttaatatag gacgccgctt agacaatgta
    25681 gaatttttta ctggctcgat tgatgatgtt cgaatataca atcgtgtatt atctataaat
    25741 gaaatacgaa gcttaagtgg ttacaatccg atgcaaacaa gtgtttggag ttcaacgcct
    25801 gcatcgagtg gcttaaaatt atattttcaa tcggatagtt tgagtggttt agccaataac
    25861 gctactgtat cttcatggac tgatcaaagt ggaaatttaa atactttgaa tcaaggaaca
    25921 ggttctaaaa tgccaacgtt cctaacgaat ggattgaatg ggagaaatac tttaaaattt
    25981 ctttctgcta atatgcaaga actcaacaat aatgccacag ttggtttagg agggactagt
    26041 tttagtattt ttgctgttgc tcaaccgctc tctcttggaa tcgatcaggg aattattggt
    26101 ataagtaatt cctgtgggaa tgataaagta ttgggagtgg atacaacgaa ttttctatac
    26161 ggtggaattt gcaatttaat ctatttaaca tcttcatcct tttcaatcac aacgattcca
    26221 aatattttta cagaaaccta ccagcatggg ggtggaggaa atttaggcaa tttatatcag
    26281 gctggaaatt taatcagctc acaagtttca agtgcaacac ctttctctgg atcaacagtt
    26341 ttagccatag gaagacgata tgcggcagct gcatattgga atggacatat ttcagaggta
    26401 ttattctttg atagcgcatt gggaaatgcc gaccgagaat tagtagaatg ctatctatct
    26461 agtaaatatg gaatagggct tggacattct tgtccttaaa aaaagaacct taatcttcca
    26521 gttttttcaa aataggacaa tctggtcttg agtctccgtg acaattatga accagatgac
    26581 gcaatgtatc cgccatatcc ttcatttgtg cgagcttttt ctctaattct tttaagtgct
    26641 tagaagctaa cgtcttaact tgtaagctac ttctagattt atttttccaa agactaacca
    26701 atttttttat atctggcaaa gaaaatccaa gttctcttgc tctcttaatg aatcgtagat
    26761 aatgaacatc tgaatctaga tagattctgt aacctttatc cgttctagca actttgggaa
    26821 ctaaaccaat atcctcataa tgacggatta gtttagcact cactccactt tctttcgcta
    26881 attgacctat attcataaac cttcccataa ctagaaggtt tgtaaattta tacaattttt
    26941 ttttaaaaaa acttattttt ttctactgag ggcttgacat tccaatcgtt ggaaggttta
    27001 cactgacagt agaatcaaaa agttagattt aaaccattta acattttgaa aagatattta
    27061 ggagaataaa aatgacatca acatatcaaa tagaaggaat gacttgcgga cattgtgtaa
    27121 agcaagtgga aaaggaattt tcaaaagcag gaatcaatgc aaaagcagat ttaaaatcta
    27181 actctgtcag catagactcc gaactagatt ctaacgctta cagtcagatc aagactgcct
    27241 tagaagagga gggatattcg cttggaaaca aaatttgaag cgaaagtaaa aatggaagat
    27301 tccaaagaga ttacacttga tctctttgga atgacctgtg ccaattgtgc tctacgaata
    27361 gaaaaaggtc ttcgtaaaat ggaaggcgta gaagaagcac gagtaaattt cgcacgagaa
    27421 actgctttcg taaaaatgaa atcatccatt cagtcggaag atttgttttc aaaagtagag
    27481 tcattaggat acaaggcatc agaacattca gataaaaatt cagaagatgc agaatctaaa
    27541 cacaaggaag agtttaataa acttaaaaag agattctata tttctgctat tctttcgctc
    27601 cctcttttgt atacaatggt ttcccatttc acgtttctat ctttcttacc gttacccgct
    27661 ctactcatgc atccatggat gcaatttcta ctcgcttttc caatacaatt ttggattgga
    27721 tttccttttt ataaaggcgc ttatagagct ttaagaaata atgcggcaaa tatggacgtt
    27781 ttagttgcat ccggaacgtc agctgccttt ggatatagtt tagttgcaag tataacaaat
    27841 ggacttagaa gtggtgacat tctattctta aatgcattga atcattttga ccatattgga
    27901 catactgcat ttccttctct ttactatgaa acctctgctg tattacttac ctttatttta
    27961 ggaggtaaat ggattgaaac aattgcaagg ggaaaaagtt ctagtgctat caaagcacta
    28021 ctcaaattaa aaccgtctac tgctagagta aaaaaagatt caaactgggt agaaattcca
    28081 attgaatata ttcaagtaaa tgatttatta cttgtgaaac caggagaaaa aattcctaca
    28141 gatggaattg taatagaagg ctctagcaca gtggatgaat ctatgttaac gggagaaagt
    28201 ttgcctgttg agaaaactgt tggaaacaac ctgttaggtg gaacggtgaa tggaaatggt
    28261 gccattactc taaaagtgga aaagattggt tcagatacag ttctatcttc tatcattagg
    28321 acagtcgaag atgcccaagc aaccaaagca ccacttcaac aagttgccga taaaattgca
    28381 aatatctttg tgcctgtcgt ttttatctgt gcgattctcg actttatttt atacttcttt
    28441 tggttagatc caaataactt tacaggagca ttggaaaaat caattgccat tctagtcatt
    28501 gcatgtcctt gtgcgttagg tcttgcgact cctgtctctt tacttgttgg aactgggaaa
    28561 gcggctgtta aaggaatttt attccgaaat gcagatgcct tagaagccat tgctcaaatt
    28621 caatcgatag cttttgataa aacaggaaca atcacagaag gaaaacctta tgttgtcaat
    28681 ttcaaaacat caacggatac gattgattat ccactccaaa aaattgcaag cgctgagtct
    28741 ggttcagaac atcctcttgc aaaggcgatt gtgaatttag caaaagaaaa aaatttacca
    28801 ttatcaaaat ttgaaaatct aaaaattgaa ttcaatggtg gaatctctgc aagtgttgaa
    28861 acaagcaaag tctatgcagg aagaaaatct tatttagaaa attttaattt caaaatacca
    28921 aatgatttag taaatatcgg aattgaatgg gagaaagcag gtagcactgt tttttatgga
    28981 gttgtatcag acacagaaaa tcactttatg gtatttgcaa tagaagataa agtaaaagag
    29041 acaagtaaac ttgctattga aaaaattaaa tctcttggta tagaaccaat tctattgaca
    29101 ggggataatg accatatctc acagaaaatt gcaaaagaag ttggcattca aaaagtctac
    29161 tcttcgctgc gaccagaaga gaaagcagaa aaaataaaac aaatccaaaa agagggaaaa
    29221 aagattgcta tggcgggtga cggaatcaat gacgcaccag cacttgcaag ttccgatata
    29281 ggaattgcaa tgggatcagg aacagacgta gccattgaaa ctgctggaat tgtcttagtg
    29341 aaaggagatt tacttcgttt aattgacgtt attcgaatta gtaaacaaac agttaaaaat
    29401 atcaatcaaa actttttctg ggctctctgt tacaatgcaa ttggaattcc tattgcgggc
    29461 attggactcc ttgccccttg gctcagtgga gctgcaatgg catttagttc catttcagtt
    29521 gtagtaaatg cactttcact gaattggaag aaggattaaa actatgaaaa aaattccaaa
    29581 catacaccct ggagaaattt tagaagaaga gtttctaatg ccattggaaa ttaccgcata
    29641 tcgtttagca aaggaaacag cgattccgca aacacggata agtcaaatca taaaaggaaa
    29701 acgaagcatc actgccgata cggcaatacg tttttctaaa ttctttggaa caacgccaca
    29761 gttttggtta ggacttcaaa acgattatga ccaagaaggt aaaagaattc aattcaatac
    29821 caaacttcaa agaaatactg gctgtttaga gttaaaagta atttattgac taatcaaatt
    29881 tttcttgctc tcttcggatt tatacaaaag cgttttattg aattatcttt tcgaggaaga
    29941 gtatgttaca aatatttata atccttttat caattttaac tttttcaaca tttgcaaatg
    30001 actttgaaaa tgagacagaa gacttattag aagcttgcgt taagggaaat atcaagtctg
    30061 ctaggcatca tttagaaaat ggtgcttcgg taaacgcaag agcgaattca ggaagaacac
    30121 caggaagaac acctatcatg tgggcagcag ctagagggaa aaaggaatta gtcaaattgt
    30181 taatcgatca cggagcagag gtaaatgcaa aagatgattg gaaaggaaat atgacaaaac
    30241 cagatggaac agaaggctgg acagctcttt tatttgctgc ggattcagat catgaagagg
    30301 tagtgaaaat tctgatcgaa aaaggtgcag atgtaaatat taaaactaaa tcaggaataa
    30361 cagctcttgg ccttgctcat ctatggaaaa atgaagaaat gattaagatg ttaaaacaag
    30421 caggcgcgag agaatagatg ctaaatcaaa tacagcagct tttcaaatac aataaagtag
    30481 ctaatttaaa atatatcaac gccttaaata atggaattga atatccagat agagcacatg
    30541 agctaatgat tcacatttta aatgcgcatg aaatctggtt agatcgaatc aatccaattc
    30601 aaaataaaga atcagttctt ccctgggata aaagaccagg gataaataag atagatttga
    30661 acttaacgtt atatgataga actgataagt ttttaaagga taaccaaata gaaatcattt
    30721 caagaagaaa tgttaaatac caaacaacaa aaggggattt gtttgagaat tcgatcattg
    30781 atattttgca tcatataatc aatcacggat cttatcatcg ggggcaaata tcgattttcc
    30841 tccgagaaaa ggatattgca cctccgaatt ccgattatat catgtatgta agagaattat
    30901 aatttaccat aaagcgtctc tgagtccctg ttctaactct tcttgattct tattagtctt
    30961 atcctgttgg tgtctagatc gcctatgatt tttattttct cctgaacgtt tttgaatccg
    31021 ttccatttcc tttcctaaac gtttctgttc atcgggagta aggacaggct ttctcggtcc
    31081 tccttcatca gcaggtaaat atcctccgcc tcctccatct ttatcatcct ctcctagttc
    31141 attcccatcc tttgagtcat tcccttcctt tccattatct cccttatctt tttgaccatt
    31201 tctgtcctcc ccgttatttc cattttcctt tccatccttc ccagtgttgc ctttgtcttt
    31261 agaatctttt ccgttctttc cattggaatt tttatcttct ttgtcgttag ttcctttctc
    31321 tttggagtcc tttccatctt tccctgtatt gcctttgtcc ttagaatctt ttccgttctt
    31381 tccattggaa tttttatctt ctttgtcgtt agttcnnnnn nnnnnnnnnn nnnnnnnnnn
    31441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    31501 nnnnnnnnnn nnnnncttta tctttggact cctttccgtc tttccctgta tttcctttct
    31561 ctttagaatc ttttccttta tcctctttat ttttggagtc tttttcgttt ttatcacctc
    31621 cctctttttt atttgaattc tttgaatcct tactatcctg tgtcttatca gttttttgat
    31681 tttgattttt ttgatcttga ggattacctg aattatccgc taattgctga tcagcattct
    31741 cggaatcctg gtcttgttga tctgaatctg atttattttg atcttgtttt gactggtctt
    31801 gggattgttg acatttattt tcttgcaatt catctaattt ggtcaattca gattcaatat
    31861 attgagaatt ctcccctgca gcttttttat aatctcctgc atttggtaaa tcgaccactt
    31921 ccttaaatcg attcatagct tgctcaaata gaacacgtgc cctttctaca tcacacgttt
    31981 caggggctaa ttctttttta ccccaaagat ataagacctg tccatcatta tagatagctt
    32041 ccttgaattc aggatttgct tctattgctt gttgaaattt ttcgtgtgca gaaggaaaat
    32101 catcctcttt tatgagtgta tttccaatgt tatatgtagg aatatatgtt ttgtaagaat
    32161 aagaaatact tttatgaaaa gaagttttag aatcttcata gttcgattca cttaaatgtt
    32221 gaattccctc ttcgttgtat ttgtatgctt tataaaattc acagtttgta tagaataata
    32281 aaaaaaaggt aaggaggaca aattttttaa aaagagaata actaaaattc ataataaaat
    32341 aagattccta tttacaaatt gaaaatcaac tacatttcga ttcctatata tctaagataa
    32401 gataaaaaga aatctaaatc cattttaatt cttgagaatc ctagactctc tccaagcatc
    32461 aagcaagctt caattagtga ctttccccaa atcaacatat ggattcaact aaaatgattc
    32521 aatttgtcca atgtcattta tgaatatcta tcattcttca tctcttgctt attatcctta
    32581 tccaatttta tataaactca tctattctag aagtaaatca catccgaaat aactgttctc
    32641 tagaaataga atgatctttc aattcatgga agttttttat gaggattttt ctccaaagaa
    32701 agaatatttc cagagattca atggattttc atttccagca aatatttttg ctcatacact
    32761 tggtataaaa taatattacg atatgcgata tatgattcat attttagaat tgacttcatc
    32821 agttcaaaaa aaaacaacaa tactatttgg ttttcatttc agtaaatcca tcttggagct
    32881 taattttctc cttaatatac aaatctgccc tatctaaata cattttagct gctttgtctt
    32941 cagggttcgt ttttaatatt tctttaaaaa gttttttcga atctctaaat ttatttttga
    33001 aaaaaagatt taaagcagat tcaaattttt cttttgtctc ctgttttaat ttttctactt
    33061 ccgattcttc attgtctaat atttcataaa ttgtaacttt ctcttctttg cctttaactt
    33121 ttacaatgtc tagcttccta aaattataat gagttacatc ggttaacttt ttaaatgttt
    33181 gctcggctat gatgatatgg gctccatagt gttttgttaa tccttcaaca cgagatgcaa
    33241 ggtttacagc atctgaaatt acagttgctt ccattctctg ctcttcgcct atcgttccaa
    33301 atatcatttc tccggtattt atcccaatac caatttggat aggtagcaat ccctcctcga
    33361 ctcttttcga attgaatttt tgaatggagt gaagcatgga gagagcagac ttgatagcat
    33421 catctggttt tatcggaaat aatgccataa ccgcatctcc aatgtattta tcaatgaatc
    33481 cattattctc gcgaattaca ggacttatat ctcttaggat atcatttaaa aattcaaaat
    33541 tctcctccgg agacattttc tcagagatag aagtaaacga tcgaacatct gaaaacaaaa
    33601 ctgtcatttc ttttttgatt gcatcaccta atcgaacaga agtgatctct gatttattta
    33661 gtattccaag caattcaaac ggaacaaagc gagcataaga attcattaat tctttttgct
    33721 tctcaattag taattcgtct gccttttctt tatctaatcg caatcgaatc aatttctctc
    33781 tcatagcgag agataaaaag attgcttccc atatagagca aacttgtaag agttcgttgg
    33841 aattccagaa agcctcattt gcaaaacccc aaaccttaaa acgatgtaac gttaaacaga
    33901 ttaggagggg aatacaagcg gttagatagt aattcgcagg accaaactta aattgattct
    33961 tataaaagaa agcgatccaa actaaaaaga aacaaaatcc aattgtataa gcaatttgaa
    34021 tttgtaacaa ccagtaatag cttgtaatag gagcgagtgg gatagtaagc aaaaaccaat
    34081 aatttaatcg aaacacttta ttaaagtttt ggattgtatc ttcatttgat aagaatttct
    34141 ttgcaaacaa tccagaaaac aaaacatata aaggcatggt aactaacgga aatatattcc
    34201 caatactcgg atatttaaaa aatacccatt caaaagaaag accattcact gaactaatcc
    34261 ataacgagaa actcaaaata gataaaatgt aatatatgta aattgattta tatattgaaa
    34321 aataaatgat taaattataa aatatcatga ctagaataat accgatatat ataccttgtg
    34381 caaaattcac attatttata taatcaataa aggctttatg atttcggata acgacaggaa
    34441 ctataactgc tccttctgtt tcaactcgaa tataaatagt ggcttctgta ttttgaggta
    34501 tatgaagatt ataggaaaag tttttatatt cgaaatctct tccatcttga aagctaactg
    34561 tatatttttg atttcgttta tctaaaccaa aattattatc ccctgaataa ataggagaat
    34621 ctcgccaaaa aattttctga tcagaataga tttcaatttt atcaagtaac ggataaggaa
    34681 tttctaaaat ccattcttgg aaatttttag attcatttct aaccttaaat cgaaaccaat
    34741 aaggaagtgc tctctcacga atcttagcag gaacatcaga acaagactgg aaaggaagat
    34801 ttaactttgt tattgttgaa aattttaaat cacgatttct atcctttaat acttgcatct
    34861 ccattggaag atctttgtca cataaatcat acttttctag aaggtcgttt agaatgagtg
    34921 tagggatttc atttgtctgt ggatgtatgt tataaaaagc aaaaaaaaga gttaatagaa
    34981 aaaagcgttt cataaaagaa tagaatccta tttaatttta taaatattcc taaaaagcaa
    35041 caattactac aatatatatt aatttcaaac tataaaaaaa ctagtcattt ctgactatag
    35101 actctttttc tttttcatgc gatagttgga ataattaaaa acaggagaaa atagcatgaa
    35161 aacaacaagt aaacattata gtttttggac aaagattaga gactttcaaa tagacgatac
    35221 atctgccgcc tttcgattct ctgataaatt agcaaaggaa caaggctggt caaaagaata
    35281 tacagatcgt gtcatagatg aatataaaaa atttattttt ttaatctgtg taacaggtca
    35341 atcttgcaca ccatcagaag atgtagatca agcatggcat atgcaccttg tctacacaaa
    35401 atcatactgg attgattttt gtagaaatac gattgggaga gaaattcatc atactccatc
    35461 caaaggagga tcaaaagaaa ctgataactt taagtttcaa tacgaagaaa caaagcgtct
    35521 ctatgaaaat tattttgaag aggagactcc aaaagatatt tggccagaca cagagagtag
    35581 attccaattt tactttagaa gaattgattt acgagaatat gtattgattc ctaaaccacg
    35641 tattcctaag tttgctagat taggtgcttt cttaagcttt ggatttttga acagtcattg
    35701 ctctggcaca ggaaacggat tcgggggctt tcttttgagt attagcattg taattggttt
    35761 tattcatcta cttggattaa tatttacaga ttcagaggat gaaaaaaatg taagacgaag
    35821 agaattaaat agagaagcac ataacgttca tgacttagca caaggcgaaa gtttttcgga
    35881 tggaggtatt gctggttgtg gtggtgatgc ggggggatgt ggaggaggct gtggtgggtg
    35941 cggaggatgc ggctaagata aaggaaacaa taaaagagat aagaaatgaa acagatagca
    36001 attgttgaga atgaagaaaa ataccgtaac attgtccgag aggaactttc ctctcgaact
    36061 cactggagtc tatcgttttt tacatccgca gaggaatttc taaatgataa aaattacaat
    36121 cggtttgatt taatctatgt agatataaat ctcattcata tgaatggaat cgatttagta
    36181 aatacagttt gggaaaaaaa tccggaattg aaaatggtca tgttgacagg actcaacaca
    36241 gattcatata ttttttctgc cttgcaagca ggcgcaatag gatatttact aaaaacagaa
    36301 ttatccaatt tagtatatgc gactgatatt tttctagaag gtggatcttt tataaatcct
    36361 gtcatagcgt ttagaattca aactttcttt aaaaggaaaa aatccaattt aaaagaagaa
    36421 ctaacgcaaa gagaaattca aatcatagaa atgattgtgc aaacagaatc cacagaaagg
    36481 atcgcagata ttctaaagat atcgattaat actgtaaaaa accatattcg aaatatttat
    36541 acaaagttac aggtcaatag ccgatccaac ttggtaatta aagcaatgga aataggaatt
    36601 ggttatcaaa aaaataaatg aaaatactct tttatatcct tgtctatttt ctaattttga
    36661 tttttgaaat tttcccaaat gacttttctg aaaagagtca tatattttat ctagaagata
    36721 aaaattctga attcacggta aaaaatctgg gtgaacttcc tttcaatgat tctctagaag
    36781 tttcaggatt ttctaattca acttggtgga tcaaaattga caatatcaaa acaaattcca
    36841 tagtctccat tgaatatcct cttttagact ttgtgacatt tcactacaaa gacaaacagg
    36901 atggtggttg gaaaaagata gaaacaggtg accacttcga attcaattca agacaaatta
    36961 gaaataaaaa ctttgctttt ttattcgggg ataatattga tttacctctt ttcgttcaaa
    37021 tcaaaacaaa tggtccagtc tttattccta ttcagattta ttcagattta aaatatatcg
    37081 aagagacaaa tagggaaagt tatctatggg gaatttactt tggagttatt tttatattcc
    37141 tattttatag tttcatttct tttttttcag tcaaggattc cacttattta ttttatatga
    37201 ttcacattgt aggttttgga atgttacaag ctgttctaaa tggaatagca ttccaatttt
    37261 tttggccaaa cagtccatcc tttgcaaata tctctcctaa tatatttcac tccatttctc
    37321 ttttatcggc tctacttttt tcaatgaaat ttttaaggct aaaagaatat ctatcgcacg
    37381 gctataagat atgtataggt ctagtattaa tttatatttt gatttttcta ttttctttca
    37441 attccaatct ctataaacat ttattaatta ttcagactta tcttatcatt gctagttctt
    37501 tttttattgt atcatgcagc ttgctaacaa ttcgttctgg atactcacca gccaaagttc
    37561 atttaattgg atgggtattc ttattcctag ctatcatttt aaatcgttta aaaacgttag
    37621 gctatttacc tcataatatt atttcctctt atggagttct atttgcttcc atccttgaaa
    37681 tgggattgtt ttcgatcgca ttaggttttc gtattttaaa aatgcaccaa gaaaaggaat
    37741 ccatagaaaa agagcgtcaa aaattgattt ctgatttaca tgacaattta ggaggtgacc
    37801 tagtcgatat aatttataat ttaaacaaat ttgctaataa gtccttaatt gaaaaatctg
    37861 atatagaaac tttgaagaat acagcagata aagcacttta taatttaaaa acgagaataa
    37921 gctcgaaaga ggaaagcatt ttgctagaag acaacttcat tgatgcagta aggctattat
    37981 tgattagccg ctaccaaaat gcgaaaagaa atgtaaatac tagtgtaagc gagaatgcag
    38041 aaaatttttc aaacaataat cctctttcag taaatgagtt aagtgatatt tatttaatct
    38101 gtaaggaaat ttgcacgaat gatctaaagt atggatacgg tgtaagcatt tggaattttg
    38161 attttgaaaa tgaatatttt tctatacaag ttttaacaaa cacaaactat acagataaaa
    38221 ataaagaagg acagggaacc ttaaatttaa aaaaaagggg aaataaatta ggagccacta
    38281 tcgaagttaa aacaagcgaa agtaagttca atctaatatt taaaatgaaa ataaactcaa
    38341 tcaattttcg ctctagtaaa aactaatttt taattattga aaggatgcat aagctatttc
    38401 tttgtatata aaaacattta atagagctac tagtattaac gatatgcgat ataatttatt
    38461 caataaaaat agtcaaaacg gactatttta aaagaaaaag aatctgataa aataatgaaa
    38521 agttgaaagg agaaaagtat gagcttagat aaattagaaa aagaggagtt aaagatttgg
    38581 caaaagatag gattttttct aggtcctttc atctttttta gtatgttgtt ttttactcca
    38641 tcttctatta cagaaactgg atctaaagga agtctcacct tagaaggctg gaaagtcgcc
    38701 gcatgcggaa tctggatggc aatctggtgg gttacggaat ctgttccgat tcccataaca
    38761 tccttattac cgataccact atttttttta ttaaatatat ccaacacagt tcctaaatca
    38821 gtaagtgaag gattaaaaga aaatggagta aataaatttt tctctgaaca aattctagca
    38881 ccttacgctg atcctattat ttatttattt cttgggggtt tcattttggc aatcggaatg
    38941 gaaaaatgga atcttcacaa aagaattgca ctcaatatta taaaaattgt aggaaccaaa
    39001 ccttctcaaa ttattctagg atttttaatt gctaccgcat ttattagtat gtgggcgagt
    39061 aacgcatcca caacaatcat gatgcttcca attgcaatta ccgttcttga attactcaaa
    39121 tcaaaattac cttcttccga aaaacataat atagaaaact ttggagtcgc tctgatgcta
    39181 agtattgctt ttggtgcaag tatcggtggt ttaggaacgt taattggaac accacccaat
    39241 gctgtgatga aagctttttt cgataagaat ggtcttgatt ctataaactt ctctaactgg
    39301 atgatattcg gagtatcctt gatcgtcatt tctatacctt ttacatttat cctcttaaca
    39361 aaaattattt atccaattaa acttgccgca atagaaggca cagatgaaat cattgaaaca
    39421 gaattgaaga acttaggaaa attctctctt gccgaagctc taactgcttt ggtaattgtc
    39481 gcaactgcaa tgacttggat ttttagagga gcttatgaaa gcaaccatct ctatatcaaa
    39541 aacaatttaa aagacggcgt aattgccatg acaggtggaa tttccttatt cctcattccc
    39601 gtgaattgga aggaaaaaaa attcctcctt gaatggtccg atatgaaagc aattccttgg
    39661 gatgttcttc ttctttttgg tggtgggcta agtctagcag gcgccgttga tcgcagcggt
    39721 ctctccgatt ggatcggtgc tcaaacaaaa tccctactag aaggtgaaac aaacatctat
    39781 ctagtcgtat ttgtagtaac tacaatggta atgttcttaa ctgaattcac aagcaatacc
    39841 gctacaatta ctctcttcct gccaattggt ttaggtatcg caaaagctct ttccattgat
    39901 ccacgactta ttttaattcc cgcaaccatt gcttcaagct gtgcttttat gctacctgta
    39961 ggaactccac caaatgcaat cgtatttagc agcggttact taaaaatacc tcaaatggcg
    40021 aaagctggat tctgggttaa tgtatttttt gccttcttga tttgtattct tatatttacg
    40081 ttaggtgtaa aagtattcaa aatttcttca taatacactt tgtttggaaa ttatttatgt
    40141 atcaagacat agctgcacca aagcaaagaa ttatagaagt aaaaatcatt ctggctaaaa
    40201 gtatagaatc tacttgctga gatcaaaaga ggtctttatc gctttttcta tactcttata
    40261 tgaaaagaac gttcgaacaa aaaggtagat ctttttaaaa accaagaact ttctactgaa
    40321 agatttgcct ctgtcgcaac tacattattt aaatcagtaa tactttgtat ctctgttaat
    40381 ccaaatgtat ctagttctaa taaaaagaca aacaaatagc accgggattt tccgcatgct
    40441 agagcaagtg atccaagttc gatccaatca caattgtcaa cccgaaagct aatacgaata
    40501 agatccaagc ctttattttg atattcagtt ttaacttttc tagggattgg aagaattcca
    40561 gaaaaattca gagagcgata tcgaaaaagg agagatttaa agtagagttt cagacttcca
    40621 ttttctttta tcttccgttt tagaatcccg attagatgtt ctggaacgag aagggaggaa
    40681 gtagttttat caaactgagt ttgttgttta tctaagcgat aagatttatt ttcaaagaga
    40741 atcatatatg cacctaaagc caaaaaaaac ttcccacgag gattttattg gagtagcgtt
    40801 agtataccgc acatactgag tgcgggaaca ctaagaagtg tttagaccga acacttattt
    40861 gtgaatgaaa aatgggaaat tatttttgac gttctgtata gaaaggagaa attttctacg
    40921 agtatgtgcg gctaaaaatg aattttttac aaaaaaattt agatttttag cgattttttt
    40981 tggttttttt agttcgaaaa tcagaatcgg ctttctaaac ttttaaagcg gttttgactg
    41041 aaaaatttta attcattctc tatcaacttt cctaattctt tttataaaat gatatatggt
    41101 tttttattaa agacattttt ggaaagttat ggataacaaa aattagaaag aatgctaata
    41161 agaaataaat cgaaagatca atttttccag atgttttaat tttagatctt gaaaatgccg
    41221 aagtttcttt taaatatgga tttagatcct ccacccattt acctttttca actacaagat
    41281 attgaccgtt agttgcttca gctatttgtt ttaatatttt atctgagcga tgtgttttaa
    41341 tattcttgat aatcttctga tcgacttctt tacgtttagt tccagtagag tcatccgttc
    41401 gatttttctc catcatctct tttttttcct cttctgtata agaaacaaag aaattgactc
    41461 ccccaccttt ttccgttcct acaccgatcg tatgcactac tacattattt ctttttaata
    41521 actgtaattc ttcctctaga ttttcatctt tgtcttctgg aacctcacca tcactcagta
    41581 aaatgacttg aatatttcct tccgactcat atacaatttt taaaacctct tgtagagctg
    41641 atttaaaact agagccagtg ctttcaaaat tatgaactaa taacgtatgc acaaaggtct
    41701 taatagttat gatatcattt attggtaatg aatgcactgc aggttttcca ctgaatgtta
    41761 tcaaaccata tgcataatct ggatttgcat caatcagttc ttcagagaat atctgcgcct
    41821 ctgcaaaacg atcttctgga tatttttttg taacaggatg aggacttgta tcgttcgata
    41881 gcatactaaa ggatccatct agcagtaaaa tgactttacc ttttcctgaa gaatcctcta
    41941 ccatcccatc cattctaggt cctgccacag ctattattaa caaaagaccc attgcaaaga
    42001 taattaagat atgcaatttc aggtgattta aatttttata ctcagtaaat accgaagaaa
    42061 atcgttttga tacgttatta tccacccatt caaatgcaat tctctgttta cgatatagat
    42121 aaattataaa agcaatccaa ggcaaaaccc aaaagaactg aagtatattt tcaaaatcaa
    42181 tcatctcata ggtctcctaa tgagcagtcc atatacaaac atccaaagaa agaaaaactg
    42241 taatccagca aaagcaaagt atcttgaata ggaatgttta agtctgctct ttttaatttc
    42301 taatggggtt ctagaaagtg ttcctaattc agaaaatatt tcagctaata cattttcatt
    42361 ttttgctcta taaaattttc catttgctgt ctttgcgatt tctctcagtt gtttatcatc
    42421 gagtgaggtt actagtattt tgccactagg agttaaataa ggcctacctt ctacatagac
    42481 ttcgattggt ttctcgcccg ccataccaat tacatacaat ttaatctgct gctctaaaac
    42541 atattttgct gccagaattg gatccactcc ataagagctt tccccgtcgg taataagaat
    42601 gattgcctga tctctctttg gtttctttgc tttctgtaaa ccatctgtag ttgcaagcaa
    42661 cgcatctcca atcgcagttc ctccgctctc cgaatgatcg atcactttaa aagaaatact
    42721 ttcaatcagc tcaatcaaga cagagtgatc tgtagttaat ggagtttgtg taaatgtatc
    42781 ctgtgcaaaa atataaactc caattcgatt gcctccgctt ctttttacaa aatcttttgc
    42841 aatctctttc atcgcatcca aacgatttgg tttaaaatca gcggcttgca tactagcaga
    42901 tacatctaat acgagggcta tgtcaatacc atcttcttga attaaattcc tttctgtaac
    42961 agtatgtgga tctgataatc ctataataag gctaatcaga attaacccct ctactagata
    43021 agaagcataa agataattaa tccttttatt gtgcggagtt ttgtattgta atggtgcata
    43081 ggaagacatt ttcttttttg caaagaaata gcgaacataa agatagaaag gtattattag
    43141 taaaaaccaa agcgcgaatg aatagctaaa gtgaatttcc ttcattagct aggcctcact
    43201 atctcattac tctttggttt ttcattttgt tttgtttttc taattcctga agcaattttc
    43261 gcctcataaa aaatttcatc aaagtcctct ttttcagggt gctgctttcc aaattgtaac
    43321 aaagctaatt tcagaaagaa atttgtaata ttcttatctt taactctcct actaatttct
    43381 tctgaagtca tctcttcaat atctataccc attttagatt caaaatgttt tttcatagta
    43441 gaggataatt catgtaaacc gcttcgacga ttctctgaat attgattgtg tatggtctca
    43501 atcttttgtt ttaaaacttc tattggttca ggaggaaatt taggctttct tttttgaatt
    43561 tctctttgca tgcttttaaa ataacgatag agccaataaa gtgtaatcag taaaatcgga
    43621 agaagtatcc agttacgaac atcttggttg taacgtaaat ctagtatgtg aacgggtggt
    43681 gcgattccat ttggcaaatc tgctaaaaga aatatcaaac gatcaccctc ttttttaaat
    43741 gaaaaaattc cttcaacgct tttcctatat cagctgtagt ggaaaaactt ccaaccgaca
    43801 tgcgatactt aatacttgca tttttaagat attctttcat ctgttctaaa ttccccaact
    43861 cccctggatt tacaaaacca aagttctttc gatttccttc aatctctggt gaaatcgcag
    43921 gaaaaaaaac ttcttttgta aactgatact caagtgggtc atagatatgc atcatattga
    43981 tatcatgctc aactctcgca aattttaaat catcaggcaa gtccctatct aaaaaatcag
    44041 aaatgataaa caatataaaa tgactgcttt taaatttctg aactgcatgc aatacacttc
    44101 taatatcaga ggtagcttcc ttacccttca tctcctctgt tttctgcaaa aaagctttta
    44161 tagtcctaaa caactgagac ttgccacctc tcggtcttac tatttcaagt gcttcattat
    44221 tgaaaataat gtaaccaatt ttatctcttg attgcattga ggaaaaagca agagtggctg
    44281 ctaattcaat tgcaaattct atcttggttc tatcctgata acctgtcatc atagatggac
    44341 taacgtctag agcaatcaaa acttcccttt ctctttcctc taaaaatgtt tttacgtaag
    44401 gctcacctaa tctagcagtc atattccaat caatcattct gaccgattcg ccataaacat
    44461 attttctagc ttcatgaaac tgaagacccc taccaggaat gattgaagtg taatcaccag
    44521 caagtaaaga atgtgcattc tttcgagcaa tgagttcgat ctcctttgct ttttttaaaa
    44581 gttcagaaac taacatatta aggaactttt acttttgata gaatttcatt tataatctgc
    44641 tctgtcttaa ttccttccgc ttccgcataa tatgttggta aaattctatg tcttaaaacg
    44701 ttatgagcaa ttgctttaat atcttcaggc attacggaat ctcgaccaaa cattaaagca
    44761 cgtgccctac ctactaaagc cagtgcgata gacgctcgag gagaggctcc atattgaatt
    44821 acattttcta aatttagacc ataagcctta ggatctctag tagcttgaat taaatcagtg
    44881 atgtaagaaa taattttatc ttcaataaat attcccctac aaatcttctg cagattgagt
    44941 atgtcgtctg gatttaaaag tttttctaca cttggaagct ttgtttcttg gattaccatt
    45001 ttgacaacat ctgcttcttc tttttttgtt ggataattca caattaactt catcaaaaaa
    45061 cggtctaact gtgcttcagg aagagagtag gttccttctt gctcaacagg attcatggtc
    45121 gcaaaaacaa agaaaggtct aggaacagga aatgtttctt cgccaattgt aacctgtttt
    45181 tcttgcattg tttcaagaag agcagattga actttcgcag gagcacggtt gatttcatcc
    45241 gctagaacaa agttagtaaa aataggacct ttatttacct caaagcttgc cttagattga
    45301 ttatagattc tagttcctac aatatctgca gggagcaaat caggagtaaa ttgtaatctt
    45361 ttaaattgca cattacagat attagcgaat aaattgacgg ctctagtctt tgcaagacca
    45421 ggaacgcctt ctatcaatat atgcccatcc gctaatatac ctaatatcaa tccatccact
    45481 aaagattctt gaccaacaat tcctttgtga atttcttctt taattttttt aatttgagct
    45541 actgcttctg cattcttcga caagtgaaac tcctttccaa gacttatata gaatgatatt
    45601 ctatttgcaa gtagaatttc actctaaaaa acattctata aatttttcta atggtgatct
    45661 gtcttttact atttcaccaa ttattgatgc tgtatatcca aaagattcta aatcatgtag
    45721 gcagtttttc acattcgatt cagggagtgt aaacaagaat ccaccagctg tttgaggatc
    45781 aaataaaata cgatagacga tactctcctc ggtaaaactg atataaggtg agaaggactt
    45841 ccaatttgat ggatataaat tactttggat ctcaggatgt ttctttaaaa tctcttcaac
    45901 gcctcttact aaaggaattt tatttaaatt gattctagct tttaaattcg atgctttcat
    45961 tatctcatac aaatgccccg ccaaaccaaa accagttaca tctgtgcaag caccaatttt
    46021 atattttcgt aatacttcta ctaaatgaaa gtttgattgt aacataaact ggatagcagc
    46081 atcaataaaa aaaggattcg ctttgttttg catatccgct gcaaaaatca aaccagttcc
    46141 aattggcttt gtgattacaa ttgcatctcc ttctttcgca ttttttttgg agtaaaattt
    46201 aggtgtatca gagaacccac tacatataaa gcctacttca aaatttctcg caagtccagt
    46261 atgccctcct agtaactcta ctccttctct attaaaaacc gaaatggctc ctgccaaaat
    46321 ttgaaacaca tcctcttctg atttttcaac tcctgcttct gcatttataa ttgcgtgggc
    46381 acaatgaccg ttaacccctt tagaatataa atcattcaga cagtgattcg cagtaatttg
    46441 accagcaaga taataatccg aaatgattgg ctgaaaaaaa tctactgatt gaattagatt
    46501 catttttttc gaaacttcaa tcacagaaga atcttctctc tcttttaaac ctaccaaagt
    46561 ttttaatttc tttttttcct tcctgttttg ctctaacgtc atttggtatt tctcaatatt
    46621 atccaaagcg tatttaataa tttcagatcc ggccttagaa tcacaacctt tgcaaactaa
    46681 actatcctga gactctgatt gagtcatatc agtcatttta tatggcaatg attgaaactt
    46741 ttccatgaaa tcttgatcaa tactatcttt ccaactccag atccactcag aagcgcctaa
    46801 tgcatatttt cctctagaag ctatcgccgt tccatctcca atatttaaaa gtgtcaaaaa
    46861 atttttttgc ggtttatatt taattaattt cttacctttt gataaattga taatattctt
    46921 aaataatggc tcactttgcc taactgcaaa aactcctgct ttgggtctac tataattctt
    46981 catactagct atatcaccta ccgcaaatac atttggatgc gaaatagatt caagatagtc
    47041 atttaccaat ataaaaccat tctcatcttt cattaatccg ctatccaaaa tccaatccgc
    47101 aggctttgga ctcatcgctc cgatgataaa atctagattg taaatatttc ccttgccact
    47161 taaacattca aaatattttc ctttattgga aatttttcga aactcctcat ttaaatgtaa
    47221 aattattccc ttttgattta aaatttttat tactttatct ttcactcttt gattatgacc
    47281 atttaatatt tctcgccctc tctgaaatat atgaatttca aaatctggaa taatcttcat
    47341 tatccgctcc cttgcagtca gtgctgtctc tattcctcca gccccagcac ctaatatcgc
    47401 tagacgaaat ccatttttaa aatctccctt ttcgattttt tttaaaatag atatccattt
    47461 ttctaaaaaa ggcagagctg gttttattgg tgtaatgtat tctcttccgg aaatatctcc
    47521 aatattaggg acgattccaa tattcatcga aagaatttca taaggaatgg gtggtctatt
    47581 ttccaatagc acatgctttt tatctaagtc taacccaacc acattagcat tgacaaatct
    47641 agctccagaa aaattacaaa gatgtcttaa atcaattaaa cattcctctg ctttatattt
    47701 ccctgcaata tatccaggta gcattcctga atagggaaca attgaattgg cagatattag
    47761 cgttatgcgt aagtcgtttt tctccattcc caattttctt aaaaccggtg catgagactg
    47821 accgccacca actaaaacca aatttttcaa aatattatcc tctcttattt ttcaccaatg
    47881 gctttccact tccatctcga taggatccag ttattattaa aattaaatca ataaaagacc
    47941 agattccaaa tccgcctaac gtcattgcct gaaaggttcc aataataaaa tttccagtat
    48001 agaaacgatg cagaccaatg aatcccgcaa aaaaacagag tagtagcact actaaccaat
    48061 cgaatccacc agaataaaat gagcctttcg cttcataata
//
LOCUS       DAOQOS010000034        47609 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_291_length_47609_cov_6.769735, whole genome shotgun sequence.
ACCESSION   DAOQOS010000034 DAOQOS010000000
VERSION     DAOQOS010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 47609)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 47609)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47609
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_291_length_47609_cov_6.769735"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..366)
                     /locus_tag="PLF66_09410"
     CDS             complement(<1..366)
                     /locus_tag="PLF66_09410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010570315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HMZ64298.1"
                     /translation="MEQNTIYQSKNKIRIVTATSLFDGHDASINVMRRILQSSGAEVI
                     HLGHNRSAKEIVDCAVQEDVQAIAITSYQGGHIEFFKYMYDLLKQRNAGHIKIFGGGG
                     GTILPDEIQELHAYGITRIY"
     gene            complement(366..686)
                     /locus_tag="PLF66_09415"
     CDS             complement(366..686)
                     /locus_tag="PLF66_09415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002739062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64299.1"
                     /translation="MLDKKSYIRVWSAFDVNEIKRHLLLIDDLYGSCANCKQLGLNYL
                     KDKECPGCKTVFKYIATNLKGIEDVKKILNRIQDMNLQLTLIEKEDYIHSTAKDAVKD
                     LFKN"
     gene            1243..2550
                     /gene="dnaA"
                     /locus_tag="PLF66_09420"
     CDS             1243..2550
                     /gene="dnaA"
                     /locus_tag="PLF66_09420"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HMZ64300.1"
                     /translation="MESIWPQILEEVSKQIPAMYFEPFISQLSFVEFKDDNLIIKAPS
                     LVIKNHVEKKYHSVLSDAAEKIGGSKLKIQIHTDSNDIPIANFIDNKFKDDSYPFNPD
                     YTLDKFVLGDCNRMAYMACKEAIESPGKLNPIYIFGKVSTGKTYLLHAVGNALNQSPN
                     RSVKYISISDFLSEFVFTVQSRQAMDSFKAKYQSYHTLIIDDIQNLNSSAEKTQEEFF
                     SIFNYLFDRRRQIIIASDRPIGELPINEKLKSRFITGFQAEIKAPDESVRIGILKQKS
                     IENNLQLSDTSIQFISDNFQTDTRALLGCLNDIQLYKKTFSLLFVSDEKVKELLESRL
                     QKAQDLDLNHEKIINLVCERYTQSKKDILSKSRKAEYIIPRHICMYLLFELCNMNKTL
                     IGRFFNTKHTTVISAINHIKQMMQDDVNFKRNILSLKALFEYK"
     gene            2892..4196
                     /locus_tag="PLF66_09425"
     CDS             2892..4196
                     /locus_tag="PLF66_09425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64301.1"
                     /translation="MKIFFITLFILLISLTQCSAKQTKQTKAEKIRVFVTIDQSSSEN
                     KKFLEVIKSSIISSISLTDSHKFLDEEYEQYYLLHTLEIAQSIGDRELETFLNDMINL
                     RNQETFNGIHIIINTQKLNTSTDLHLKFIAANGKHSELILPINKTSLEFYNSLQEKII
                     LTLEPDNEKAIETLRASFILINDIEAYNFYLQGKSEKRSKTIYGYLNAIKLFSEGLKK
                     DPINILLLNEMVQTMNLIISNLNEIISKKEIINKKEIIAEARSEVNRNIMIIQTLFEN
                     FNLYAKNNPDQYQKILEYYNQSPLKKEFDFKIKNFRNELDTLLVKWQNERFGLTEVKT
                     RDLKNLESSYAPLIANDFFQTITNSRISINDIIRALIKYSHEHPENHYYIHYFKNFSQ
                     LMSLLNKIDYIKINPDASISYQLLRQEYIEYIRKYLSIIKKG"
     gene            complement(4448..5416)
                     /gene="trpS"
                     /locus_tag="PLF66_09430"
     CDS             complement(4448..5416)
                     /gene="trpS"
                     /locus_tag="PLF66_09430"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008593792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HMZ64302.1"
                     /translation="MRILTGVQPSGKLHLGNYFAVMDKMIRYQENNDLFCFIANLHSL
                     TSFKSKEALVENTFEAACDFFALGIDPDKSTFWVQSDVPLVTELTWYLSMCINVNQLH
                     LAHSYKDKTAKGINPTGGLFFYPILMAADILLFDSQKVPVGKDQKQHLEFTRDIANKF
                     NHDFGTTFLIPEPEIDEGTAIIPGTDGQKMSKSYGNTINFFDKESSLRKSVMSIVSDS
                     AGIEEAKDPDKSVIFAIFKLFLNETDLKTLRTRFETPGLRYGDIKKELFSTIMDYFAP
                     YRKKREELVANRDYVQSLLLKGAEKANKVAIPVIERVRERLGIKRI"
     gene            complement(5421..7253)
                     /locus_tag="PLF66_09435"
     CDS             complement(5421..7253)
                     /locus_tag="PLF66_09435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016546719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64303.1"
                     /translation="MNPASRTEAHKVFAALYRKKRSPEHQQLIDDAITRSNDVFIRID
                     LIRKIDEEQERKERHKDEPVQSESKTESMKALPSSGNPTAKPLVPKSQTSAQIPAVHD
                     NASGGFMDKLFGGGNSIAKFAKETKALDLGLFALKPTISKNVEKIFRSLKEDEIISTI
                     QALKYSEQVGWRIWTPLVYNVILNYNRFFNAFISLDSLFRDEISAEVFLGRSTKMQMY
                     YARMLNRPDTKEIILEKVPALIRNEAKLASKLDSIYTGLNYGLSLETRKPSLTDAIVA
                     FHVVMNRKLLPWSEIEKTLNVSVIDDKKFNATQEVAKQIELATAKLSNDIQAKLNIKD
                     ELDFLKKNYFSFGEGGKLSFEFLNPIIDDYVSHYYAENMQTDVLKSNFKNTPHKLLQL
                     VCRDLQSNYIPLVEGYIKLDLNQVKDVLIMQTGLFFPEVDRINNVLRNLDSFNRKFPS
                     FQYGFQNYAENLAKGTQDQIETQLLKMLTDASDFFHKFAMKMTTVIENHMLAVKYEES
                     GQINEKTLATKEKVIEEIKIMQRFIPFAEGKVVAQGRLAGKTVFQVLFEMTRLLYNYA
                     VIFKDAALLSKLGQSAKNEAELQKLYADYERLTAKPYNLDKREI"
     gene            complement(7257..7823)
                     /locus_tag="PLF66_09440"
     CDS             complement(7257..7823)
                     /locus_tag="PLF66_09440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004788865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HMZ64304.1"
                     /translation="MKLIFSIWLLFFSISLSAESGEELLNSIIGTMNSFESFRANISI
                     DGLTGSISYKRPGSLNVKFSDGRVLSGNGRYLWIYSPARGIAGKQDLKGLTGGLGGLL
                     SGYESVTSSGKVLKLKSENKYYEEIIVSVTPNNIIKSLRLRAKGRPDFMEISFSGVQT
                     NIGLSSSIFNFHPPANAQIVENPLNQRE"
     gene            complement(7903..9546)
                     /locus_tag="PLF66_09445"
     CDS             complement(7903..9546)
                     /locus_tag="PLF66_09445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64305.1"
                     /translation="MQQITTKKIALYSMLLFLIFFISGYLFFSKYVESLFLLKRENLS
                     LGLISVAASLDSSDLKSLSEQEFQFSEYKKTWTKLKRLQDKLKLSYIYIIIPRELNQF
                     YFAYDTANNPDSFSSKKLSFRKYSDPPLEIYDSIVTDEVVFVNGFQKNQWGVFTSGFY
                     RIQKEGKLVAIIGADLDIFSFLEIKKRSNVIFLLSISLGLIVTLFLFYTLYFHLYLPL
                     NKLSHEIERISNGSMESIVQKETGDFSDLFRDLENIKKQIRAKSELADQASIQFVEKL
                     SQRKEYLRIALASEEKLLQEKEEILAYLSQLASSLFESKGSLGNIRIQNFAKQNYYNF
                     IDQKRFSSSGLVFISRQMNFLGSKHLFFFYGDTEKRYMQGLISSLFIYILVYSLLIKQ
                     EQNKRLVLKEADRWLRDLFWQIETLLGKSKMNMHVKGTAGVISQETGKMLFFSIGKEN
                     IFLSRDENLIDLSEKFSSIPSEFNSESKYLQNEFQLSSGDILYFCNAESDTLETKEKI
                     DTIVQILAQSKGEIDKIQYTFTQSNLAIREISLLQIQYE"
     gene            complement(9561..9923)
                     /locus_tag="PLF66_09450"
     CDS             complement(9561..9923)
                     /locus_tag="PLF66_09450"
                     /inference="COORDINATES: protein motif:HMM:TIGR04452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04452 family lipoprotein"
                     /protein_id="HMZ64306.1"
                     /translation="MRYIVYLLLFLSNCIVLDTLNLSVPESVSGKEAKDTIVTAAITG
                     AAISSAIDGRVTMDSLLSFVSDKLAGVKENAYYDKYDVDECAAKAQLINVVTIRIGGF
                     NCKLKEHKTFTNWPVPIF"
     gene            10213..11118
                     /locus_tag="PLF66_09455"
     CDS             10213..11118
                     /locus_tag="PLF66_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HMZ64307.1"
                     /translation="MKLTYILFLLLEILSLIHCHKEQKITITGSETMHSMMLLLANNF
                     SKENKNYIIDVKGGGSSEGINELIGGLTDIALSSRDLTEAEFEKLNSKQTLESLVIAY
                     DGAAFVVHPTNSVESLTLEQLSDIFSGKIKNWKEVGGANQPITVVIRDNYSGTAHYIR
                     EHVVRQLDLGQASYFKNKQKDYTSEAVTLINNEEISDFVNKNPNAIAYMGMGIAHIKS
                     NLKLLKYSRTSSDEAILPTIKSITERKYKLSRALKIFYRTDRSGSKIDEFIKYILSEN
                     GQKGVLQSGYLRSTLPEVEVSAQKK"
     gene            11120..11434
                     /gene="cutA"
                     /locus_tag="PLF66_09460"
     CDS             11120..11434
                     /gene="cutA"
                     /locus_tag="PLF66_09460"
                     /inference="COORDINATES: protein motif:HMM:NF015077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HMZ64308.1"
                     /translation="MNFIFIYITCKDREEAIKIGKELVESKLAGCVNIFPSMTSIYNW
                     KENIEVADESVLIVKTKESLFKEICDLVSKIHSYTTPCILSFPIQNGNEEYLQWLSSN
                     MK"
     gene            complement(12638..14092)
                     /locus_tag="PLF66_09465"
     CDS             complement(12638..14092)
                     /locus_tag="PLF66_09465"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5982 domain-containing protein"
                     /protein_id="HMZ64309.1"
                     /translation="MLKVILSLSLFLIFFLSKLDSEDLETDSSLPFPIDKSRRIDSEE
                     LKYKRENSFITGLPSVSSDPITGVWYGGSGYFTQNGKKNNPLFAYAPYVYRVTADIYQ
                     SSVGARFYGAGVDLPYFQNTPFRISLYGFYDRNLRSQYYGVGESTLHPLSYHPRNDDS
                     QPLVTNAEYSQREEAISFRRPGRVLNGPTYVTDQKYNEFDAENSGFFVNIDRTFWKVF
                     RFAVGADGYRMIVRPCDGQIVKAKDPFWGETDFSNLNVVLPTPNAKTKLTEDNESNKI
                     IGYDGGYTNLMKAGIAYDSRDFEPNPKKGVFAEVNLVKASRAWGSNFDFQREFLQIKI
                     FYSILPKIFSELVFASRVALTNVTGNIPFYEYRHMWSIDGGPIYGLGGGKTMKGYKQD
                     RFIGNTMGFANTELRYRFAKYNIGDEYFTFQIVPSVDLGRVWNSIDRLNWQGYKYSYG
                     LGLKIIWNQSTVLSFDYSVSREDKQFFMNFGHTF"
     gene            complement(14135..14788)
                     /locus_tag="PLF66_09470"
     CDS             complement(14135..14788)
                     /locus_tag="PLF66_09470"
                     /inference="COORDINATES: protein motif:HMM:NF024247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HMZ64310.1"
                     /translation="MERKDFFKKGIFKAIKKAVEATEEVYDTLHESVSQKAPAEDISN
                     IPADIEYLNRLPKLTKPKKIIHNIKFPPGAITDKERFQKTCTGCGDCIQACPYNALFP
                     VYDSKKKKNFPHLDPNANPCLMCFDFPCINSCNYKALKPLKKDTKPKFGKAKVVADRC
                     INHSKDELVCDVCKNVCPIENVITYQKNFLPKFSKDCTGCGICVQSCPPVVKAIIIK"
     gene            complement(14799..15206)
                     /locus_tag="PLF66_09475"
     CDS             complement(14799..15206)
                     /locus_tag="PLF66_09475"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HMZ64311.1"
                     /translation="MSNSNVDFSSPNFNHIGTELQIKTRIKPEPNQPVDGAILDIVGD
                     LNLYSTPALKQILGNLVDAGKIKIFVNTENLNYIDSSGLGAFLAIQSKLMKMSGFIKL
                     CCPTKQVLAVLELTKLKSMLKVNSNLADALKGS"
     gene            complement(15218..16372)
                     /gene="galK"
                     /locus_tag="PLF66_09480"
     CDS             complement(15218..16372)
                     /gene="galK"
                     /locus_tag="PLF66_09480"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002972301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HMZ64312.1"
                     /translation="MDTIINLKKNLINAFGENNDTIRLFRAPARINVIGEHVDYLGGF
                     VLPAAIDFQVCVAIRKNDQGKINLYSEQYKESVSKDTPSYDKKYLWANYVYGVVDELK
                     KEGFTITGFDMAIDGNIPQGAGLSSSAALEVVTGYALSSIFHLSISREKIALIGQAAE
                     NNFVGMKCGIMDQYIIANGKKDHCILLNTDTLSKTYHTLNLNGHEFYLINSNVKHSLQ
                     DSDYNNRRKECESALKKVQSLEPDIKNLYDLPLDTVLNKFNFSEKEEKRVKHVLGEKD
                     RTNKLLDSFAQGKIKNAGECLYGTHESLSKLFEVSCDETDFLVQALQKENVIGARMIG
                     GGFGGSILVLDKIGNREKVEAKVKKDYFEKFNLNATFYGFQISDGVSELQ"
     gene            16476..17831
                     /locus_tag="PLF66_09485"
     CDS             16476..17831
                     /locus_tag="PLF66_09485"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004787212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HMZ64313.1"
                     /translation="MNIKIDSKFLKSYFPESLLSKNLEKSILKKELLHNKKGPGSDFL
                     GWVDLPSQIKESDLKNIQDVADEINSHSEYLVVIGIGGSYLGARAMIEANTKSFHMFE
                     NHKNKKTKVLYAGHHIDSDYHADLLDFLQDKEFSVNVISKSGTTTEPALAFRSLLSLV
                     EKKYGKENIKKRIFATTDAKKGALKKLSDEYGIKTFVIPDDVGGRYSVLTPVGLIPIA
                     ASGISISEFVKGAREMQEFLAKEKDPVKNLSCYYAALRNTLYESGKKIEIMVNYNPKM
                     HYFTEWWKQLFGESEGKDKRGIFPAGVDFTSDLHSMGQYIQDGERNLFETVLHIQEPS
                     RDTIIQEFAGDPDGLNYIAGKKLSYIGEQAINGTKLAHYEGGVPNIDISIPNINPSIC
                     GELVYFYEYACGVSGYMLGVNPFDQPGVEDYKNNMFALLGKKGYEEMKIKVEGKLKSL
                     I"
     gene            17852..18280
                     /locus_tag="PLF66_09490"
     CDS             17852..18280
                     /locus_tag="PLF66_09490"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010679598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HMZ64314.1"
                     /translation="MIKALAKEDFKFYTTLRVRYAEVDLQGIVFNANYLTYIDTAITE
                     YFRAIGYTYLDFVKKHSMDFHVIRSVVEYKAPAHFDDELEICLRPEYEGVKIFWKFAI
                     FRGNEILTFGEVVYASFDLETKKLKKIDSNVVTTLQLTEN"
     gene            complement(18326..19033)
                     /locus_tag="PLF66_09495"
     CDS             complement(18326..19033)
                     /locus_tag="PLF66_09495"
                     /inference="COORDINATES: protein motif:HMM:NF012659.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64315.1"
                     /translation="MLFKIFLVISVIPILSILAEPSCELDSSKEKLYRLELTVPATFC
                     SKANNDPSCATFPKKSILQLHGLWPNYTKGYPEGICDSSECKNQSEKLGKFCKYPSPK
                     GLYSSEMWKKMSDYMAGTEKCLERHEWVKHGICSPLEPVTYFKWALEKTKLISGAFEP
                     LKNRTISRQEVNEAVEKNLPDLVGSIRMNCKGKNLSSVYVLYEWGNTPGNPIKTKGGE
                     NGFGNCPKSFFISSSPE"
     gene            complement(19048..21048)
                     /locus_tag="PLF66_09500"
     CDS             complement(19048..21048)
                     /locus_tag="PLF66_09500"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HMZ64316.1"
                     /translation="MNPLATFRKSINIQILTISAIAIALTGAMVGLLSFRLAKVVYEK
                     KIFKEDLKTLIELKKVKIENEIEKALKVSQLLASDPELIEWFQGRESNSTLKKSTESK
                     MINIAKLLEYTRIFASNVSTNSYYYFENHLNNKKNISIERKIINNSSKEDEWFFKFWN
                     SNQIYEVNISKSKSEAREDSENTYAYINTLIQDGNKKIGVAGVGVKLTLFVKRFFEKD
                     SNNGKTLLVDTKTMTVKISSDFSEIDKQVQNFISEHLNSEQSLDESNNPPIISNDEKG
                     DSVFLIFRHFSINKSLAVVYVVPYEQVMSEFSIIRHATIASTVIIVLFAFVIFSFYSR
                     KITRPILDLAVIAERISGGELELRYITKTRNEIGRLAASFNAMTESLAEKNKSLEDYS
                     KNLQEKVYEHTYELAKQKRELELKNRSMQKELEMGQKVQGIFLLPFNSRTDWYEIYAT
                     SYSSKELGGDFFRIYETGKNEVWMIIGDVSGKGVGSSLIMTASVSLLDQIQGICKSID
                     DLARDFNSQLYHLIGSNKGTAPGFFVTAGCAFLSKEENRYFIYTVNAGHEPPLLFRND
                     KFSVLPSGGRAFGIREDTNYNLFKTELFKGDILFYVTDGMFEQRNQNGDFFEFENIKK
                     SIGKYKNESVEIICNKLIEDLDDFSVNVPQEDDRTIVAFRIL"
     gene            21180..21731
                     /locus_tag="PLF66_09505"
     CDS             21180..21731
                     /locus_tag="PLF66_09505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64317.1"
                     /translation="MEDFSLNDSGELYIRWNLNTTYAKLQLNELIGALIQEYRHTGGV
                     YDAFIENDGKLVGNEKIVIIRNLRDVIQLSVCLKNQLSTKYIKKSTQEEKIICKFIDE
                     YSFQLTYKYTNSEIEDISSVKIWYDNYFKKEILKFTDDFKKALDDKVISEEEAIELVR
                     ILDHIILGAAILFNKLTFENLTN"
     gene            21806..23191
                     /locus_tag="PLF66_09510"
     CDS             21806..23191
                     /locus_tag="PLF66_09510"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HMZ64318.1"
                     /translation="MSYKKIFLTFFSLITIESLQSVPEVKVIPIPGNINTEMQEFAPS
                     LTSDGKTMYFYSKRNNSKYTDLYKSSLINGRWTNPIDLRGLNSAYDDQSPFISEDEKF
                     IVFSSNRDGSIEFQLPSGKIGVSRDLYYSENINGKWSKPSSLSDKINTEEMEENPFLH
                     ESDFYFTRYPFGNPSEAKIWKAKIIGNHLQEPEELPSPINLEGTSNIAAVISKDGKYI
                     YFASNRPGGYGGYDIYRSKINKDGSYTDPENLGPEINTQGDEAYMVIDKANNAFYFCR
                     KNLNESYDIYTALILKDEEEAKVATKENPIRDKSIEETKINPPKDKIEIPSVVELPKK
                     EIPKKDDVTQTLKEKKKLTLNNVLFDTNSSELLSESFPVLNQIVDFLKEHPDTKIKIT
                     GHTDLTGDTTLNKILSLERAESVRTYLHSKGIERKRMIADGKGSTQPLINNVEPESNR
                     VNRRTEFQIVD"
     gene            complement(23204..24313)
                     /locus_tag="PLF66_09515"
     CDS             complement(23204..24313)
                     /locus_tag="PLF66_09515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64319.1"
                     /translation="MKFIFLLITLILANSVIAQENSDVKPKQKFFIPATEFINPGFKI
                     TLSGTSNASHSHSRGPTSPEGYYLLSAVAFRNQASLNTAGGQFQRDAFLTENIENYSY
                     LNQKNDQVSPLGKLGFSYTTKSGDWSFEVSIRAKNYSGNYALGNNVPFEYYPGTFQYN
                     WREGQYAVIRNHALVNFLMVQPELGIREISEKYNRNSDLNSYPFPKNYSSVSETSRSY
                     SAQSGLTFHFKIIDSFRIKLTGKIFQALSGEVNNQRTDFRNDGISIYARNINSKLSKV
                     DTYGNEIEGEIGYSLKKLNLFLGYNLTTITKAAHRDSNYIPIITTNENLMNEYIRYYL
                     ADTIYALSRSTEDSVSDKPRHQIIKYFYFGASINF"
     gene            complement(24317..24649)
                     /locus_tag="PLF66_09520"
     CDS             complement(24317..24649)
                     /locus_tag="PLF66_09520"
                     /inference="COORDINATES: protein motif:HMM:NF024546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRL domain-containing protein"
                     /protein_id="HMZ64320.1"
                     /translation="MKRIYILFLLAILFLTSCITSPGAAMFGATKHHLSAGNKNTITS
                     NQILKVGESCSYSSHFGNLFILYYGVGGSINEAMDKAGITKIAVIDRSSMSIYPFYYE
                     DCVLVYGE"
     gene            complement(24649..24975)
                     /locus_tag="PLF66_09525"
     CDS             complement(24649..24975)
                     /locus_tag="PLF66_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015680583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRL-like family protein"
                     /protein_id="HMZ64321.1"
                     /translation="MKLLLFVLILFLNQCLMLPIMQKTNGPVHGLIFTQTKFPGEINP
                     NNDVPATNKGEGCLHYIFFFFMWGDASAGSIALQNKIKRIATIDHSTMNLFGVYGNYC
                     TLVKGE"
     gene            complement(24977..25363)
                     /locus_tag="PLF66_09530"
     CDS             complement(24977..25363)
                     /locus_tag="PLF66_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002976819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRL-like family protein"
                     /protein_id="HMZ64322.1"
                     /translation="MTSWKSLLLISVILLSLFNCTAANYWNVTNSTNNTNPMPEYNNF
                     YYLTFKGGWLFHFVKAPGSIGNAESKLVGKQCSYSMLYLIAVGDSSIESAVKDGKITK
                     IATVSYEQLGILSFVYHRFCTIVTGE"
     gene            complement(25596..28229)
                     /locus_tag="PLF66_09535"
     CDS             complement(25596..28229)
                     /locus_tag="PLF66_09535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64323.1"
                     /translation="MKFRFALFTFFLFVTNIFSAPYTGWEIGNNHSVLYINDLSVFDT
                     RDTIRYLPQKLNSDQIQQYATYIAEYYISRGDKDGLANLANLLKSDRSNSYEFIANLI
                     LVFWKIEKAETNDALFKLDEIIAKEENPLHQSIAKVVKSNLKKISIPADTKDVIKNLN
                     CSRSKPMYSFCRLIKVSVHLEHLAETSDYPHRDYLNLDRLLAPFFEEYELAHVSFLEK
                     FTFEMPSKLAYLGFAYEAVHFQKMVVNLQKISGAFDPTEHERLSFYQMLNDDLTSAEE
                     ALSYSSKKTSAITSNKNPIFLKLGAIAFLRKDYKQSMNYYLELNLKEWNKSIKNPFYD
                     DSISINGARGLISLSLWKSKSAVNAVRALNKLNTDNTITEESLFLRLRIAQIMMAEKP
                     EMAEKISEDIMYIAQGKGWKRVEYAATIMNGYCSIISKKYRKATVQFTKSYGILGGSD
                     PAFTSEWMRSSGMLIARVQGGERGNHAVSFRSLLNSMRNEEPLDDILSVKNFLDSRFD
                     TEAFFKTAVNYFTYTKNYQSLIEFLFHYQKMKFKSSSIVGKTILQLPDVNKRMKHYRS
                     FRPSSDYHYVRSIYSKLREAESSKMSSDSDLFDVNSLKDIRDPFVAAFSFNEKIYVIS
                     YNPESRDKWSISLHNSIDYRTTGYYNKIFSNIPFLDKAGNIQIFMNPLGVDLYQAMKK
                     NNALLNLRLFYRFNKGNLKSEGDLQPVAVSCDSGNEVVTNGIQYLPAEYFEGTKTFSN
                     DQRLHIWTTKDLGNRDVSSIENFEWRCQNNSTISFSKLRRRVDLRMIPNAILFSNSIF
                     PKSSLDSISLDYYFWADFWMQKGANTLYYVDRLEGDKYSFTDLVNVLTKVSQGFDDPA
                     KIQSTLKSSDKEILILTRDMR"
     gene            complement(28257..28862)
                     /locus_tag="PLF66_09540"
     CDS             complement(28257..28862)
                     /locus_tag="PLF66_09540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64324.1"
                     /translation="MSDSINEKVEEYLKQLAEVDGSFHFQSLLQDLKQKALEDEKAIW
                     FLFKSIVGDLIKDSNIWNEKTNLVVGIFYLLIDIGNEKAYALVKWYIQNFKEDTPNGA
                     IELLSTLIPSFANINSEEYFTYLKSENKALSALGFLTLFNLSMERKLNAAEEKHLYEI
                     AQTYTNDRYYTEHIVDMISFRFRKSEDSSQEESDKIDINLQ"
     gene            complement(29100..29753)
                     /locus_tag="PLF66_09545"
     CDS             complement(29100..29753)
                     /locus_tag="PLF66_09545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64325.1"
                     /translation="MFTQIIRIGYILFIQNLFLFSLFAQAKEEFIFKEKICIQDFESI
                     SLSDLNVKVSANTSELPEIKFSKTLTSPDLQSNTSLLIRNLVGQKKNPFEIIFKKPLL
                     ISDHIIEIEFNIYSNKIRSDLFLILMDNKFQFHTILITHLDFDGWKQIKLPIQNGIYQ
                     SDFLLQDKSISKVIGFNIIPAKIGEIQNTENIIVLDDMFLIRRQKYILPKLGTDRFH"
     gene            29915..31018
                     /locus_tag="PLF66_09550"
     CDS             29915..31018
                     /locus_tag="PLF66_09550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64326.1"
                     /translation="MNNIIKLFLMLSFTIALVAQSGIKPLPNDGKMDRAEKAIETIPT
                     NSVDSLKTESKTTVTTTTTTTTTAAPAPVNSAPVVTQPPVSNARAIDPNLGNTPALYI
                     NSRTAFELVTGDDLSGVDYIEYKINDGEYSRYTVPISLSKEGPATITYKATDKVGNKE
                     TAKVINLIVDNTAPTVSVKVSEPLFTNGNTQYSSPKNTYQLIAEDKSSGVKQIVYSVD
                     NEPKQDYKNEPIKLEKPGFHVINFYAIDNAGNVSQENSYIVNIDGIKPTVDIKESIPY
                     IVIANKVYAKKETVFTITAADGQSGIKQVLVKTDGAPDFVPYVEPLTFGTSGEHIIEA
                     KAIDNVGNESDIKRVVFSNDIKAPTTVIKAVSN"
     gene            31112..31831
                     /locus_tag="PLF66_09555"
     CDS             31112..31831
                     /locus_tag="PLF66_09555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HMZ64327.1"
                     /translation="MTEKTFTKLIHLFYPLDCSICGDTDLLASKISICRKCYRSETKS
                     QNPNLENLCEKCKTKINASECPYCKSRNVFFNQLYYLREKNNFETELIRKIKFGNNPY
                     LTNYFRIGLGKRIKILKNNSYKNIIQIPSNQKTLRTRPFAVSKPISDYLMKKLKLEVI
                     KPIVKTSSELQSGKSFRDRFLHAQTAFSVIAKYKNQLNGNYLLVDDVFTTGATVNEIS
                     KLLLINGAKSVDILVLAKGKV"
     gene            complement(31834..32850)
                     /locus_tag="PLF66_09560"
     CDS             complement(31834..32850)
                     /locus_tag="PLF66_09560"
                     /inference="COORDINATES: protein motif:HMM:NF025684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4384 domain-containing protein"
                     /protein_id="HMZ64328.1"
                     /translation="MKRILFIIPFLFLFSNSPTSNDMGISIETNSKIYRPGQSVKINI
                     KLKQASYLYILTLQSDGNINLLYPNEEEQENMVFNEEIRIPDEKKDYEFLAGDRIGKD
                     IIIGIASRNRIKKLHKSKYWSKPVLKGMNPSNMAWLKKLTKRLPQDQWVSAETPIFIS
                     KDGVTESNGVPTKKESKIITPSSTDVIPTDFKKDLHDFQQNEVLYGLFKLPSSVRNTE
                     NKKGSLLVMKDPDLNTYKLRVTPYLTNPKDFCDYSIVSFTNPRKVSSTYASIEVVPGE
                     CSLKEVNAEQKDFWKKITNFYIQYQYKKNGALKGNVWMIAQDGKYNQKMSKFAVKDEF
                     KFLE"
     gene            32945..33814
                     /locus_tag="PLF66_09565"
     CDS             32945..33814
                     /locus_tag="PLF66_09565"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HMZ64329.1"
                     /translation="MLEDKNTPKAFELFRISIIQLTILSFSFILLSIFSQLAMATVAY
                     QNRLPENLLDLSEEERTTFQNKIAKEFESLAKNHPEKIADEFIEATTNTTPSLLLIQN
                     ILWFVSFLIPAYFVFTRFLKDDVQCLNDELSFTHINRGLMAGISVFIILMLVSLVFYV
                     FNFKPKVNHFQTKLFTNIKGNFYLLAWCIYTVGIITGIMEEFLFRGLMLNHFVKKGYA
                     KEGLMITSFIFGIMHFSFDASPVIPAILTFVGVLFGSIYLQSRNIWTVMATHACYNSL
                     GLITAYFLGDKLL"
     gene            33811..34560
                     /locus_tag="PLF66_09570"
     CDS             33811..34560
                     /locus_tag="PLF66_09570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64330.1"
                     /translation="MKNFLQRVYIVFLLSVPFSPLFSSESFESSASVEDANLKVVKLL
                     NKITDNSIRSMESTGGFNYRYKSGLISPFRFNVYVGKISKKSEDSIIRIESTKNGEAK
                     LLKTILEVELNKSAADYKFEQVINPKYHIVSQSLNLITPAASVLYNSYNSPFYTNSDT
                     ILKAGTYVLIDLFIVALAAVYINNSKVGNKNIYDDLLNKKGPGKDFRDYPQREFILAA
                     LLIPRIYRSIEAFHDTAAQNRIAELSYTYRF"
     gene            complement(34676..35551)
                     /locus_tag="PLF66_09575"
     CDS             complement(34676..35551)
                     /locus_tag="PLF66_09575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004784141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HMZ64331.1"
                     /translation="MKPKVLGKGLGNLIGSSGRSSESSDTSTNSPMREIKLSEIKVNP
                     NQPRKTFSAESISELAETIKQHGLIQPIVVTKTNDGYELVSGERRYRACKEAGFHKIP
                     AIVRNYSEKETLEIAIIENIQREDLNPIEEALAYQQLTEKLSMKITEVAARVGKNRST
                     VSNMIRLLQLPDSVKELIKTRKLSEGHARPLLSIGDKKKLETYAQQIVDKNLTVRDVE
                     EYVASLWETKTTLSTKRDNTDPTIMNVETKIRNKLSAKVKISHNAKVGKGKIVISYNS
                     IDEMDRILSNMNIRQ"
     gene            complement(35560..36324)
                     /locus_tag="PLF66_09580"
     CDS             complement(35560..36324)
                     /locus_tag="PLF66_09580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HMZ64332.1"
                     /translation="MGKIISISNQKGGVGKTTTSINLCSYLAASGKKVLLIDVDPQGN
                     AGSGLGMDINQIENTSYEVLIGDVSANEAIQKTGIENLSIIPSNINLSGAEIDLMSED
                     RKEFRLRDALAPIRARFDFIVIDCPPSLGVLTVNALCASDSVMITLQTEYFALEGLTQ
                     LMKIISLVQENLNSSLELEGVVLTMYDKRTNLANQVANDVREYFKEKVYKSMIPRNIK
                     LGEAPSFGKPINFYDPDGVGAQSYKSLCDEVIAANK"
     gene            complement(36312..37052)
                     /locus_tag="PLF66_09585"
     CDS             complement(36312..37052)
                     /locus_tag="PLF66_09585"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HMZ64333.1"
                     /translation="MSELILPDRILTLFPKTGKQILEIFHLSKVIEYSNFLRDYNEIG
                     GFFSKSDSEFFLDRHVIESMFHIFVINSSGYIKASDEVADVGTGPGLPGYLFSCLQNS
                     PKVTLIDSQSRRLSLLEKFYKEQNYDSKVSFLYDRMEGIKKQFDLVVMRSAIRYPWSC
                     EMVYKILKQNSYFIPFLAKRVYDTNFEKTLLQNLGMIIEKDLDLNELEFLGNRHIKIL
                     KKEANPNKGFPRNWESISKEIKRISWEK"
     gene            37416..37835
                     /locus_tag="PLF66_09590"
     CDS             37416..37835
                     /locus_tag="PLF66_09590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004495925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HMZ64334.1"
                     /translation="MKKIFKVFSLLMLVLVAFVNCRHHSLADRADWIVKRISSELDLN
                     DKQKTELQRIKKEILDKKKELDLKMFPDDIVDQIRQTQIDEAKVNKSFEVNGAKRDQM
                     RVFMTKKFIEFHAILTPEQRNKLADKIGELLKKHNHD"
     gene            37828..38343
                     /locus_tag="PLF66_09595"
     CDS             37828..38343
                     /locus_tag="PLF66_09595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016758525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HMZ64335.1"
                     /translation="MTNSDFTLIVNSTKSVVLSAIEKNLAERFYYAIDDVVQETYLRA
                     YKSLTKGNFRYEAKISTWLYTIARNESLRMNDKLIREEKKIEKATSQIKEERPIEEEV
                     EENPKKVNLLDYLKMIPDKYKEILEHYINGLSEKEIADRLSIKQGTVKSRTSRGKEFL
                     RKIILMGERNE"
     gene            38336..38647
                     /locus_tag="PLF66_09600"
     CDS             38336..38647
                     /locus_tag="PLF66_09600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64336.1"
                     /translation="MNKPNRKEEITFRLNSNEWDKDIAAKVTNKYQRKVRRNRVIATS
                     AFLLLMGVSTFITFFYQNPVETEYMNLQAFQFLFSDDLFPIFDAFYQEESISFFADSI
                     L"
     gene            39046..39789
                     /locus_tag="PLF66_09605"
     CDS             39046..39789
                     /locus_tag="PLF66_09605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64337.1"
                     /translation="MKTFLLFYFLVLSILYAYTPGKWSYLDRFVVGNEKFGPVSEIVK
                     NRTGQTIYSAMYEYNVEGKLIKEKFLNAKGENDGEIVYTYDSGKVKTEEVFSPDKQSQ
                     EKKTFSYASSGNLKEIIITSASGKGVLRHKINSIQDGFVLDCETKWEEEGAQESFSSK
                     KDVLDETILNQEIFDDKKKSIGFIKYYYDKKGRIEKRENMQGNSKRMQLLTYDESGKL
                     ISFSFHVKQDENWVLVKTHYLTYNSNNTQ"
     gene            complement(39812..40792)
                     /locus_tag="PLF66_09610"
     CDS             complement(39812..40792)
                     /locus_tag="PLF66_09610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64338.1"
                     /translation="MENIKITFLIYLILQLFLTAPLFTLLVNRIFRKYLICKCDLFGF
                     FLPIFFFYAKNLEIKTSGVNKTDVVKIKVDRIFFFLNPFSLFAGRLSIYQFRIKNLDV
                     KYINHIPSIEKIKYMPREGKAIIHSGYITNGNIEIEDMSVFPIYKTDIRNIEIADALI
                     DLGNPFQLIFNSEYAYCKIDKGDVISTCKNGTGQLQVKGVTWGKLINLDILPIGFLQN
                     KIDLNLNFTHTESITRFNGTLGQINRKPQKQNQSDRKQKLEYEFQADWKEYRLPFDLA
                     LKKMVYQLLIGMNYGGAFTIVIEVIAKKFAKLLKGNTQTKNNDIILDSSN"
     gene            41436..42449
                     /locus_tag="PLF66_09615"
     CDS             41436..42449
                     /locus_tag="PLF66_09615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64339.1"
                     /translation="MKDIEVNQEVFEIKNSEIDVTSIMADIESNLKRRAIDKTEIERI
                     KQLKISAETPVGHRPFDPSHVANLFEKGISPPRFSNPKYWFVKGPLKWAIHKFTDFYS
                     LVDKKLSENRIKAFYSVVYELILLRTQYERLERKFNDLYKITLNSETENNNYQYSSMF
                     YNHDELLSEKFSNENVRIGNLISKESKTLILLPAADNFLNLLRWNKIDFHLITDKKQQ
                     LEYIQKQLTKNIEYVESIQSFTNFKNYKNILIHSNLCLLNPKTIERIIQNILEQADKN
                     TQVLLQFSNHTLQTISPFRENFQTYIEVDHLETYLRNLGFINICKHSVEKEEMQLITF
                     YTK"
     gene            42446..43501
                     /locus_tag="PLF66_09620"
     CDS             42446..43501
                     /locus_tag="PLF66_09620"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HMZ64340.1"
                     /translation="MRIFQHVDEFNERDGIGNDISGFDSIFKDLHLESNIICRINNSK
                     QKTDIYSPEDKLVFSKNDVHILHYGGTGYPIDFFANLPGKKILRFHNITPAYFFKPYL
                     DSSIYESFEKNESISLFELYSLRKNIHFLWSDSKFNQDEFFKFTGKTNKFIAEVLPVT
                     HRYPLATAKSENNFTIGFVGRLVPNKKIEDLYFTLFYLKKINPKYRLILFGKRNQIFK
                     KYFQKLDSIAEELNLLDSIELNENLDDANLRDKFSKLSVYVSMSEHEGFGVPLLEAMS
                     LNIPVIAYSSTAVTELVENAGLLFSTKDFPKIAELIDTLHSNPNIKDRLLQMQKKRIQ
                     IISNFSYKEKIQNLIKI"
     gene            43564..44616
                     /locus_tag="PLF66_09625"
     CDS             43564..44616
                     /locus_tag="PLF66_09625"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001974208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HMZ64341.1"
                     /translation="MKRIIQFSAGFNPGDAISNEMIILKSFFQEIGYDGEIFSENIGS
                     KSNRLAAKYKSYTPRKGDCIIYHHSIHSSILEFVLNLDSPKVLIYHNVTPSYFFEPYD
                     LKLTYYLKKGREELSELLGKFQLYFADSNFNKQELVDLGFENVHVLPIIYDFSRLPKK
                     EIKKSKSTKDIIFVGRIAPNKKQDDIIKVAKVLKEYFLSDFRIHLVGYCSKELELYRD
                     ELKSLIDLFDLSDHVFFSDFINDDLLAEYYQNADLFLCMSEHEGFCVPLIESMYYEVP
                     ILAFDAGAVRDTLDGAGILFKTKDFPTICECIIKILSDKDFRDKIVEGQRERLTRFMQ
                     SNHTRIIGDAIKHIQI"
     gene            44613..45623
                     /locus_tag="PLF66_09630"
     CDS             44613..45623
                     /locus_tag="PLF66_09630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020778592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HMZ64342.1"
                     /translation="MRKYFIKRLLLLFPTVLGITFVVFLISHMAPGGPFESEIAKWRG
                     LANESGGSQKQISEEEIQLLKERLHLDKPAPIAYLYWLKKIVVFDLGESRINYKKVSD
                     LIVEKLPISLTFGLSGFFISYIICIPLGIYKALHHGEGFDLATSFIIFFTYSIPIFAL
                     ALLLLYFLASGEFFSLFPLGHEVSDHYESYDTWGRLIDRLHHMFLPVLCYIFHSFAVQ
                     TILMKNSLLEEISKDYVRTAVSKGMSFKDAVFKHAFRNSLIPIATGFGSNLSLIFAGS
                     LIIELVFSIDGMGLLSFNAVRERDTDLMMGLLLVQSILSLLGNILSDFCYVLIDPRIR
                     FE"
     gene            45620..46663
                     /locus_tag="PLF66_09635"
     CDS             45620..46663
                     /locus_tag="PLF66_09635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004758480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HMZ64343.1"
                     /translation="MMLNPNTKKRLLKFKNNRRAYYSFLFLLITYIVSLFAPILVNDT
                     PILVYYNESFYFPIFKFYPEKTFGGKSETVANYKKLNKTEAFQSNKNFMLFPLISYGY
                     NESNMADLPFGVSPPTAPDKKHWLGTDDRGRDVFTRIFYGYRISLSFSLILVFLEILI
                     GTIIGGLQGYFAGIFDITFQRLIEIFSAIPFLYLILIMGSFFGRSFTILLITYGAVSW
                     IGISYYMRGEFYKLRQAQFVEAAKAFGVPSYKIIFKHIVPNALTPIVTFLPFILIGSI
                     SILSALDFLGYGIPAPNPSWGELIGQGRERLSAWWLIAFPSFALFLTIQLSAFIGEGL
                     RDAFDAKEKIAFK"
     gene            46660..>47609
                     /locus_tag="PLF66_09640"
     CDS             46660..>47609
                     /locus_tag="PLF66_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010573242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMZ64344.1"
                     /translation="MKKKNTKIVKAKETKKESQPLLQIENLSLSIAANTDLPVLQSIS
                     FQIKESEVLALVGESGSGKSLTALSITKLLPIKIFNYKTGSIFYKAKDLLKVEEEEIR
                     KIRGKEIAYIFQDPFTSLNPLKKIKEQITESYKIHISENPKEAIEKAKYLLNKVGLTD
                     LDERLNSYPGEMSGGMLQRICIASALMCDPKLLIADEPTSALDVTIQSQLVDLLLKIK
                     SENNMSVLFISHDIALVASIADRIAVMYAGQVMEQGNTEDVIKKPFHPYSDALLKAIP
                     AGIKHHDRLSTIDGIVPSPADYPIGCHFSNRCAKVYDRCE"
BASE COUNT        16539 a         7973 c         7145 g        15952 t
ORIGIN      
        1 ataaatgcga gtaataccgt atgcatgtaa ttcttgaatc tcatctggta gaatagtccc
       61 gccacctcca ccgaagattt ttatatgacc tgcatttctt tgctttaaca gatcatacat
      121 atatttaaaa aactctatat gtccaccttg ataagatgta atagcaatcg cttgcacatc
      181 ttcttgaact gcacaatcca caatttcttt ggcggaacga ttgtgtccaa gatgaattac
      241 ctcagctcca ctagattgga gaatcctacg cataacgttt atactcgcat catgcccatc
      301 aaaaagggat gttgcagtta caattctaat tttattcttt gattgataaa ttgtattttg
      361 ttccattagt ttttaaacag atccttaacc gcatcctttg cagttgaatg gatataatct
      421 tctttttcaa tgagagttag ttgtaagttc atatcctgga tacgattcag tatttttttc
      481 acatcttcaa tcccttttag atttgtagca atatacttaa atacagtttt acatcctgga
      541 cattctttat cttttaaata atttagccct aactgtttac aatttgcaca ggaaccatac
      601 aaatcatcta tcaacaacaa atgtcttttg atttcattta catcaaaagc actccaaact
      661 cttatataag attttttatc taacataaaa ctatgaatgc atttttaaat tccaaatgtc
      721 aattaaatac tgtcatttct gttgatttaa agtgggtaat acgaaaagtt tactatgaat
      781 ttattagtca aatgccaatg cttaattcag ataaaatgga ttattttttc gattttaaaa
      841 tcactacttt gttataagaa ggattgaatc ataaaaaact ctagcatttt tagaaatttt
      901 cctttcacaa gcacctacaa tactttaatt ttttttataa aatattttta tgtttttatt
      961 ttttttctaa cctaagaaat ttttaatttt ttaatatatt agaaatatct aatataccaa
     1021 tttaaataat tttctatatt agtttgatat aatttttatt ttctaaagtg tacccattga
     1081 caaaaaaaaa tatttttttt tatggctgta cacttctttt ctaaatgaaa aaaaattttc
     1141 aaagtacact ttttttattc cgaaaaacga ttttactctt tatgaatttt gacactttga
     1201 atatattgtt tttcagtcat ttggatattt ttgggaggac aattggaatc aatctggccg
     1261 caaatcttag aggaagtttc aaaacaaatt ccagcgatgt attttgaacc atttatatcg
     1321 cagctatcat ttgttgaatt caaagatgat aacttgatca ttaaagcgcc ttcgcttgta
     1381 ataaaaaatc acgtcgaaaa aaaatatcat tctgttttgt ccgatgctgc tgaaaaaata
     1441 ggcggatcga aactaaaaat tcagattcat actgactcaa atgatattcc aatcgcaaat
     1501 tttattgata ataaattcaa agatgactct tatccattta accccgatta tacgctagat
     1561 aaatttgttc taggtgattg caatcgaatg gcttatatgg catgcaaaga agcgattgaa
     1621 tctccaggga aattaaatcc catttatatt tttggaaaag ttagcactgg taaaacttat
     1681 ctattgcatg cagtcggtaa tgctctcaat caatctccaa atagatctgt aaaatatatt
     1741 tcaatttcag attttctttc cgaatttgta tttacagtac aaagtcgaca agcaatggat
     1801 tcatttaagg caaaatatca gtcctaccat actttaatta ttgatgacat tcaaaactta
     1861 aacagtagtg cagagaaaac acaagaagag tttttttcca tctttaatta tttatttgat
     1921 agaagaagac aaattataat tgcttctgat cgaccgatag gcgaactacc aatcaatgaa
     1981 aagcttaagt ctagatttat tacaggattt caggctgaga ttaaggctcc agatgaatct
     2041 gttcgaattg gaatactcaa acagaaatcg attgaaaaca atttacaatt aagtgataca
     2101 tccattcagt ttatctcaga caattttcaa acagatacaa gagctttact tggctgctta
     2161 aatgatatcc agctctataa aaaaactttt tcattactat ttgtgagtga tgaaaaagta
     2221 aaagagcttc ttgaaagtag gttgcaaaaa gcacaagatt tagacttaaa tcacgaaaaa
     2281 attattaact tagtttgtga gcgttatact caatccaaga aagacatctt aagtaagagt
     2341 cgaaaagctg agtatatcat tcctagacat atttgcatgt atttactatt tgagttatgc
     2401 aatatgaata aaactctgat tggtaggttt tttaatacta agcatacgac agtaattagt
     2461 gcaattaatc atataaagca gatgatgcaa gacgatgtaa atttcaaacg aaatattctt
     2521 tcactaaaag ctttgtttga atacaaataa agtatttttt attgcgaaag aatttcaaaa
     2581 ttcatattcc tagcaagaga gaaatgagca ttaccctgtt tgtagagata gtctaaatta
     2641 agtagtaaat gtttttttct ttgtgttcta aatgaactat ttgagtttgc gacaaccaaa
     2701 cgttcagata aagcttacat tcaatgccat cgaaaaaaat gatagttctt caaagaatcc
     2761 gatgggttcg tgtattagtt tttgttttct taatcagagt tatatcacga gttatcgagt
     2821 ttagttgtag tttttaaata tacttatcta tccaagtata gatacagtag aaattttaaa
     2881 gggagtaatc tatgaaaata tttttcataa ctctattcat tctgttaata tccctaactc
     2941 agtgtagtgc aaaacaaaca aaacaaacga aagcagaaaa gattagagtt tttgttacaa
     3001 tcgatcaaag ttcatccgaa aacaaaaaat tcctagaagt aataaaatca tctataatat
     3061 catctattag cttaactgac tcacataagt ttttagatga ggaatacgaa cagtactatc
     3121 ttctacatac tcttgagatt gcacaaagta taggagatag ggaattagaa acctttctga
     3181 atgatatgat aaacttgcgt aaccaagaaa cattcaatgg cattcatata attatcaata
     3241 ctcaaaaact gaatacatct acggatttgc atttaaaatt tatagcggct aacggcaagc
     3301 attcagaact aattttacca atcaataaaa cctcattgga attctataac tccctgcaag
     3361 agaagataat actaacattg gaacctgata atgaaaaagc tatagagact ttaagagcaa
     3421 gttttatttt aatcaatgat attgaagcat ataacttcta cctacaaggt aagtcagaaa
     3481 aaagatctaa aactatttat ggatatctaa atgcaatcaa actattttcg gaaggattaa
     3541 agaaagatcc tatcaatata ttacttctaa acgaaatggt tcaaactatg aatcttatca
     3601 taagcaattt aaatgaaatc atttcgaaaa aggaaataat aaataaaaaa gaaattattg
     3661 cagaagcccg atcagaagtt aaccgcaata taatgatcat acaaactttg tttgaaaatt
     3721 ttaatctata cgcaaagaat aatcctgacc aataccaaaa aattctagaa tactacaatc
     3781 aatcaccact aaaaaaagaa ttcgatttta aaattaaaaa tttcagaaat gaattggata
     3841 cattattagt aaaatggcaa aatgaaagat tcggattgac tgaggttaaa acaagagatt
     3901 taaaaaatct agaatctagc tacgcgcctc tgattgcaaa cgacttcttt caaacaataa
     3961 ctaattccag aataagcata aatgatataa taagagcctt aataaaatac tcacatgaac
     4021 atccagagaa tcactattac attcattact tcaaaaattt ttcgcagttg atgtcgcttt
     4081 taaataaaat tgattacata aaaataaatc cagatgcaag tatatcttac caattactaa
     4141 gacaggaata tatagaatac attcgaaaat atttatctat aataaagaag ggataatgcc
     4201 tgactctgtt attactgaat tttgataaat tcatcttata agatgactca aagaatctta
     4261 ttgaatgcga atgaaatatt aaatccagta tcgcttggag ttcggatcag agtttgaaaa
     4321 gcgatagacg aaaagtcatc tcttccttct cattttatct actcacactt actgtcattt
     4381 tccttgatta tagaaatatc tactttgaat gcttggctct atatttgtaa ggtgagttat
     4441 cgaataatca gattcttttt atgcctaaac gttctctgac acgttcaata acaggaattg
     4501 caactttatt tgctttttca gcccctttta ataataaact ttgcacataa tctcgattag
     4561 ccactaactc ttccctcttt ttccgataag gagcaaagta atccataatc gtggaaaata
     4621 attccttttt tatatcacca tatcttaatc cgggtgtctc aaatcttgtt ctcaaagttt
     4681 tcaaatcagt ttcatttaaa aacaatttaa agattgcaaa tattacactt ttatctggat
     4741 ccttcgcttc ctcaattcca gcagaatcag aaacgataga cataactgac tttcgtaaag
     4801 atgactcttt atcaaaaaaa ttaatcgtat ttccatatga ttttgacatt ttctgtccat
     4861 cggttccagg aattatagct gttccttcat caatctctgg ctctggtatt aaaaatgtgg
     4921 ttccaaaatc atgattaaat ttatttgcaa tatcccgagt aaattccaaa tgctgctttt
     4981 ggtctttacc aacgggaact ttttggctat caaataataa aatatcagcc gccattaaaa
     5041 ttggataaaa gaaaagtcca cctgttggat tgataccctt agctgtctta tctttgtatg
     5101 agtgtgccaa atgtaattga tttacattaa tacacatact tagataccaa gtaagctcag
     5161 tgactagcgg aacatcggac tggacccaaa aagttgactt atcaggatca atacctaatg
     5221 caaaaaaatc acaagctgct tcaaaagtat tctcaactaa cgcttccttc gatttgaaag
     5281 atgtcaatga atgaagattt gcaataaagc aaaacaaatc attattctcc tgataacgaa
     5341 tcattttgtc cataacggca aagtaatttc ctaagtgtaa tttgccagaa ggttgaacac
     5401 cagttaaaat tctcattcga ttatatttct cttttatcta agttataagg ttttgcagtt
     5461 aatcgttcat agtccgcata aagtttctgt aattctgctt cattttttgc gctctgccct
     5521 agtttagaaa ggagagctgc atctttaaaa ataactgcgt aattgtataa aagcctagtc
     5581 atttcaaata aaacttgaaa taccgttttg ccagctagac gaccttgtgc aacaacctta
     5641 ccttccgcaa aaggaataaa tctttgcatg atttttattt cttcgatcac tttttccttt
     5701 gtagccaaag tcttctcatt aatctgccca gattcttcat acttaactgc gagcatatga
     5761 ttttcaatca cagttgtcat tttcattgca aacttatgaa agaaatcaga tgcgtcagtc
     5821 aacatcttga gcaattgtgt ttcgatttga tcctgtgtgc ccttcgctag attttctgca
     5881 taattttgaa atccgtattg aaaacttgga aactttcgat taaaactatc tagatttcta
     5941 agcacattat tgatacgatc tacttcagga aaaaatagac ctgtttgcat aatcaaaaca
     6001 tcttttactt ggtttaaatc taattttata tatccttcca caagtggaat ataattactt
     6061 tgcaaatctc tacagacaag ttgcaataac ttgtgcggcg tgtttttaaa attagatttc
     6121 aacacatcag tctgcatatt ttctgcataa tagtgagaaa catagtcatc tataattgga
     6181 tttaaaaatt caaaggataa ttttccacct tctccaaatg aaaaatagtt tttctttaga
     6241 aaatctaatt cgtctttgat attgagttta gcctgaatgt cattggacag tttagcagtt
     6301 gcaagttcta tttgtttagc tacttcctgt gttgcattaa actttttatc atcgattacg
     6361 gatacattta aagtcttttc aatctctgac catggaagta attttctatt cattacaaca
     6421 tgaaaggcaa ctatcgcatc tgtcaaagat ggttttctag tttcaagaga taaaccataa
     6481 tttaatcctg tgtaaattga atctaatttt gaagcaagtt tagcttcatt tcggatgagt
     6541 gcaggaactt tttctagaat aatttctttt gtatctggtc gatttaacat tctagcataa
     6601 tacatctgca ttttagtaga tcgacctaaa aaaacttcag ctgatatttc atctctaaat
     6661 aaactatcga gagaaataaa cgcattaaag aaacgattat aatttaaaat cacattgtat
     6721 acaagtggag tccaaattct ccaaccaact tgctctgaat atttcaatgc ctgaatagtg
     6781 ctaatgattt catcttcttt gagtgaacga aaaattttct caacattctt tgaaatagta
     6841 ggctttagcg caaataatcc taaatcgaga gcctttgttt cttttgcaaa ttttgctata
     6901 gaattaccac caccaaagag tttgtccata aacccaccag atgcattgtc atgaacggct
     6961 ggaatttgag cagatgtttg tgatttagga actaacggct ttgcagtagg atttccagaa
     7021 gatggcaaag ccttcatact ctcagttttt gactcggatt gaacaggttc atctttgtga
     7081 cgctcttttc tttcctgctc ctcatcaatt tttcgaatga gatcaatacg aataaaaacg
     7141 tcattcgatc gagtaatagc atcatcaatt aattgttgat gctctggaga gcgcttttta
     7201 cgatataaag cggcaaatac tttatgagct tctgttctag atgctggatt caaattctat
     7261 tccctctgat tcaaaggatt ctcaactatt tgtgcattcg caggcggatg aaaattaaaa
     7321 atactagatg atagtccaat atttgtttgg acaccagaaa aagaaatttc cataaaatca
     7381 ggcctacctt ttgccctaag tctgagagac tttattatat tattcggcgt aacacttaca
     7441 attatttctt cataatattt attttcagat ttaagtttca agacttttcc agaactagta
     7501 acactctcat atccagacag aagtccaccg agtcctcctg ttagtccttt tagatcctgt
     7561 tttcctgcaa ttcctcgagc aggagagtaa atccataaat atctaccatt tccagataat
     7621 acacgaccat ctgaaaactt tacatttaaa ctacctggtc ttttataaga aatagatcct
     7681 gttaatccat ctattgaaat atttgctcga aagctttcaa aactattcat tgtgccaata
     7741 atgctattta atagctcttc ccccgattca gcagataaag aaatggaaaa aaataggagc
     7801 catatagaaa atatcaattt cattttttca acttaaagct ttatgcttat tactacaatt
     7861 aatattttac caactaattg tatgtaaact gctgaaagaa ttttattcat actgtatctg
     7921 aagcaaggaa atttcgcgaa ttgctaggtt cgattgagta aaagtatatt gaatcttatc
     7981 tatttcccct ttcgattgtg caagaatttg aactattgta tctattttct ctttggtttc
     8041 tagagtatca ctttcagcat tacaaaaata aagaatatct ccagaactca attgaaactc
     8101 attttgcaaa tacttactct cagaattgaa ttccgacgga atagaagaaa atttttctga
     8161 taaatcgatt aaattctcgt cgcgtgataa gaaaatattt tctttgccaa tgctaaaaaa
     8221 caacatttta ccagtctctt gagagataac tccggcggtt ccttttacat gcatattcat
     8281 tttactttta cctaaaaggg tttctatctg ccaaaataaa tctctgagcc aacgatctgc
     8341 ctcttttaaa actaatctct tattttgttc ttgtttgatc aggagagaat aaactaaaat
     8401 ataaataaaa agagatgaga tcaaaccttg catatatctc ttctcagtat ctccataaaa
     8461 gaaaaataga tgtttacttc ctaaaaaatt catttgtcta gagataaaaa ccaacccaga
     8521 ggaagaaaac cgtttttgat ctataaaatt ataatagttt tgctttgcaa agttttggat
     8581 cctaatattt ccaagagatc cttttgattc aaacagtgaa cttgctaatt gacttagata
     8641 agcaagaatc tcttcttttt cttggagtaa tttttcttcc gatgccaaag caattcggag
     8701 atattctttt ctttgactga gtttctctac aaactgaatg ctagcttgat cggctaattc
     8761 cgatttggct cgaatttgct tttttatatt ctctaaatct ctaaataaat cagaaaaatc
     8821 acctgtttct ttttgaacaa ttgactccat agatccattc gaaattcttt ctatttcatg
     8881 agaaagttta tttaacggca gatatagatg gaaataaagg gtataaaata aaaaaagcgt
     8941 tacgataagc ccgagagata tggataacaa gaaaataaca ttagaacgtt ttttaatttc
     9001 tagaaaagaa aaaatatcta aatcagctcc aatgattgca actagctttc cttccttttg
     9061 aatcctgtaa aaaccagaag taaatactcc ccattgattt ttttggaaac cattcacaaa
     9121 aacaacttcg tctgttacaa tagaatcata aatctcgagc gggggatcag aatattttct
     9181 aaaggaaagc ttctttgatg aaaaagaatc tggattattt gctgtatcat aggcaaaata
     9241 aaactgattc aattctcttg gaatgattat atatatatag gatagtttta atttatcttg
     9301 cagtcttttt aatttagtcc aagtcttctt atactcagaa aactgaaatt cttgctcaga
     9361 aagagattta agatcagagg agtctaaaga agccgcaact gaaatcaaac ccagcgataa
     9421 attctctcgt ttcaataaga ataaagattc tacatactta gaaaaaaaca gatatccaga
     9481 aataaaaaaa attagaaaca gaagcattga atacaatgct atcttcttag tagtaatttg
     9541 ctgcatgata gaaaattttt ttaaaaaatc ggaactggcc agttcgtgaa tgttttatgt
     9601 tcctttaatt tacaattaaa tccacctatg cgaattgtaa caacatttat taattgtgcc
     9661 tttgctgcac attcatccac atcatattta tcgtagtaag cattttcttt tacgccagca
     9721 agtttgtctg aaacaaagga taaaagagaa tccattgtca cccttccatc gatagcagaa
     9781 gaaattgctg caccagtaat tgcagctgta acgattgtgt ctttagcttc tttacctgaa
     9841 acagactcgg gaacagatag atttaaagta tccagaacta tacaattgga caaaaataat
     9901 agcaaatata caatataacg catttaaaga aatactcctg tcggtaaaat tagtagtcaa
     9961 aattctttcc ctaatcaata caccagtcag gttataggcg gcaatgataa attttaagag
    10021 aattggaaat aaatctactc tttccggatc gcttcatcca tatttccata ctatttttaa
    10081 aatcagtcta tagaaaataa agctatggaa acaagcttta aataaaatca aacaggtgta
    10141 acttgattgt aactaaaaag ttacaatcat ttgataaaat tgggcaaatt tacaaacaaa
    10201 atagggggat aaatgaaatt aacctacatt ctttttttac tattagagat tctttctcta
    10261 atccattgcc acaaagagca aaaaataaca atcacaggtt ctgaaacgat gcattcaatg
    10321 atgttgttat tagcgaataa tttttccaag gaaaataaaa actatataat agatgtgaaa
    10381 ggtggcggtt ctagtgaagg tattaatgag ttaattggag gtttaacgga tatagcttta
    10441 agttcaagag atttaacaga agcagaattt gaaaaattaa actctaaaca aacactagaa
    10501 tcattagtca ttgcttatga tggggctgct tttgttgttc accctacaaa ttccgttgag
    10561 agtttaacac tagaacaact atcggatata ttctctggta aaataaaaaa ctggaaagaa
    10621 gtaggtggag caaatcaacc aatcacagtt gtgattcgag ataattattc aggaacagca
    10681 cactacatta gagagcatgt tgttcgtcaa cttgacttag gacaagcaag ctacttcaaa
    10741 aataaacaaa aagattatac ttctgaagca gttacattga ttaataacga ggaaatctcg
    10801 gactttgtaa ataaaaatcc aaatgcaatt gcttatatgg gaatgggaat cgcacatatc
    10861 aaaagtaatt tgaaattact gaaatattct agaactagtt ctgatgaagc gattctacca
    10921 accattaaaa gtattacaga acgaaagtat aaactatcta gagcactgaa aattttttat
    10981 cgcactgata gatctggttc taaaatagat gaattcataa aatatattct gagtgaaaat
    11041 ggtcaaaaag gagttttaca aagcggctat ttaagatcta cacttcctga agtagaggta
    11101 agcgctcaaa aaaaatagaa tgaattttat ttttatctat attacatgta aagatagaga
    11161 agaagccatc aagattggaa aagaattagt tgaatccaaa cttgctggct gtgtaaatat
    11221 ttttccttcc atgacatcta tctataattg gaaggaaaat atagaagtag cagatgaatc
    11281 tgtgttaatt gtaaaaacga aagaatcatt atttaaagaa atatgcgatt tagtcagtaa
    11341 aatacactct tatacaacgc catgcatact ttcattccca attcaaaatg gaaatgagga
    11401 atatttacaa tggttaagct ctaatatgaa gtaaaatcaa tataaaagac tataaagaat
    11461 tgttttattc tttttaatta atagattcta agaatttctt tccttttatt tcaaaattca
    11521 tttcatttag attataaaaa taaaaaaccc gcttttttta ggagcgggtt tagttttaaa
    11581 aaaaattgat ctattttttt taaagaagtt tcattacttc ttttttgcgg caggtttctt
    11641 ttttgcagca ggttttttct tagcagcagc tttctttttt gcaaccattg atgtagcctc
    11701 caattagatt ttggtctttt ctttttttct aaatccgacc aatagatgtt agtgacataa
    11761 ttaatacgtc tatgaaatta tgtaaagttt ttttttattt ttttgaaaat attttttact
    11821 tctgtaaaga gtatcgcaac attatttaat tttcattaat acttaattat gaaatttatt
    11881 tctttaatga attttaaatt ttatattcat gaaaaatttt tttgaatcat aaatccttgc
    11941 atcaacgaaa aaaaaatgaa tagaattgtt ttggttaaca tctattaaac acttctactt
    12001 taaaaaaaat tttatcaaca cagttataaa caaaattata catgcaacaa tgataaatat
    12061 ctctaattac tctctattca ttttcaattt gaagttaatt cggtgtttct aaatctaatc
    12121 tctgataaaa aattcctctt tctctcgatt aaaattatta ttttcctatc tttgttacaa
    12181 agaaaccatt caaattgaat caaaatagtt ttttataaga gataaattag tttttttttg
    12241 tttcatgcag acaatttttc tttgtaacgc gactgatatt tcccaaatat taaatagcag
    12301 acaaatattt ataaagaaaa ctcttcccat actacaataa acgttgtgtg ttcaagtaaa
    12361 agaaacttaa ttccattcct tttggtaggt cttttacttc tttattatac catttgatga
    12421 attctaatct tcgatttcaa ttgtattaag ataaattata ttcgtatttc cattattggt
    12481 tgattagaaa aactattttg taaaagtaat tattaaaatt tcttataaaa taagatttcg
    12541 attcgtagat taatacattt ttagccatat ttttattgat tcaaatgaat ttagatggaa
    12601 actatcgata gaattccttt cctatctata ccttagatta aaaagtatga ccaaaattca
    12661 taaagaactg tttatcttcc cttgaaacag aataatcaaa agataaaact gtagattgat
    12721 tccaaataat ttttaaacct aaaccatatg agtatttata tccttgccaa ttgagtcgat
    12781 cgattgaatt ccaaacccgc cctagatcaa cacttggaac aatttgaaat gtaaagtatt
    12841 catctccaat attatatttg gcaaatcgat atcgtaactc agtatttgcg aaacccatcg
    12901 tatttcctat aaacctatct tgtttatatc ctttcattgt ttttccacca cccaatccat
    12961 aaatcggacc tccatcaatg ctccacatat gcctatattc atagaatgga atatttccag
    13021 taacattcgt caaagcaact cgagatgcaa agactaactc tgaaaaaatt ttaggaagaa
    13081 tagaataaaa aatttttatt tgtaaaaatt ctctttgaaa atcaaaatta gatccccatg
    13141 ctctagatgc tttgactaaa tttacttcag caaagactcc tttctttgga ttgggttcaa
    13201 agtctctcga gtcataagca atacctgctt tcattagatt tgtatatcca ccatcgtatc
    13261 caatgatttt atttgattca ttatcttcag ttaatttagt tttagcattt ggagtaggta
    13321 atacaacatt caaattagaa aaatctgttt caccccaaaa tggatccttt gcttttacga
    13381 tttgaccatc gcaaggtcta acgatcattc tatagccatc tgcaccaaca gcaaatctaa
    13441 agactttcca aaatgttcta tcaatattaa caaaaaagcc tgaattctct gcatcaaact
    13501 cattgtattt ttgatctgta acataggtag gtccatttaa tactctacca ggtcttctaa
    13561 aagagattgc ctcttctctt tgagaatact cagcattagt cacaagaggt tgtgagtcgt
    13621 cattgcgagg atgataggaa agcggatgaa gggttgattc tccaactcca taatactgac
    13681 ttcgtaaatt tctatcgtaa aagccatata aactgatccg aaaaggagta ttttgaaaat
    13741 aaggaagatc tacgcctgcc ccataaaatc tagcaccaac agaggattga taaatgtcag
    13801 cggttactct gtaaacgtat ggggcatatg caaaaagcgg attatttttc tttccatttt
    13861 gggtaaaata tccacttcct ccataccaaa ctcctgttat tggatccgaa ctaacggaag
    13921 gtaagccagt aatgaaacta ttttcccttt tatatttaag ctcctctgaa tcaattcgcc
    13981 tagatttatc aattggaaat ggtaaggatg aatctgtttc caagtcttct gaatcaagtt
    14041 ttgatagaaa gaaaattaag aaaagagaaa gagatagaat tactttaagc atgatttcga
    14101 tctaagaaat tagactcaaa ggtaaaattc gaaattattt tataataatt gcttttacga
    14161 caggtgggca agattgaaca caaatcccac aacctgtgca atcttttgaa aatttaggaa
    14221 gaaaattctt ttgatatgta attacattct ctatcggaca aacatttttg cagacatcac
    14281 aaactaattc atcctttgaa tgatttatac atctatccgc aaccactttt gcttttccaa
    14341 actttggttt tgtatccttt tttaaaggct ttaaggcttt gtaattacaa gaattgatac
    14401 aaggaaaatc aaaacacatt aaacaaggat tggcattagg atctagatga ggaaagtttt
    14461 tctttttttt ggaatcatag acaggaaata acgcattgta aggacatgct tgaatacagt
    14521 caccacaacc tgtgcaagtt ttctgaaatc tttccttatc tgttatagct ccgggaggaa
    14581 attttatatt gtgtatgatt ttctttggtt ttgttaattt cggtaaacgg tttaaatact
    14641 ctatgtcagc aggaatattt gaaatatctt ccgctggtgc tttctgtgat acagattcat
    14701 gcaatgtatc atagacttcc tctgtagcct caacagcctt ttttatcgct ttgaatatac
    14761 ctttcttgaa gaagtccttt ctttccattc tgtatcggtt aacttccctt tagtgcatcc
    14821 gctaagttag agtttacctt taacatagat tttagtttgg ttaattctaa aactgccaaa
    14881 acttgctttg ttggacaaca tagttttata aatccactca tcttcataag cttagattga
    14941 atcgcaagga aagcccccaa accagatgag tcgatataat ttaaattctc agtatttaca
    15001 aaaattttga ttttacctgc atcaactaag tttccgagaa tttgttttaa tgctggggtg
    15061 gaatataagt ttaaatctcc aacaatatct agtatagctc catctacagg ttgatttggt
    15121 tctggtttga tacgagtttt gatttgtaat tcggttccga tatgattgaa attagggcta
    15181 gaaaaatcga cattgctatt actcatatat ttatttccta ctgtaattct gatactccat
    15241 cagaaatttg aaaaccataa aatgttgcat tcaaattaaa tttttcaaaa taatcttttt
    15301 ttacttttgc ttccactttt tctctgttac cgattttatc taaaaccaat atacttccac
    15361 caaaacctcc cccaatcatt ctagcgccta tgacattttc tttctgtaaa gcttggacta
    15421 aaaaatctgt ttcatcgcag gatacttcaa atagctttga tagagattca tgagttccat
    15481 acaaacattc tcctgcattt tttattttcc cttgcgcaaa agaatctaat agcttgtttg
    15541 ttctatcttt ttcacctaat acatgtttta ctcttttctc ttctttttct gagaagttaa
    15601 atttgtttaa aactgtatct aaaggtagat catagagatt tttaatatct ggttctaagc
    15661 tttgcacttt ttttagagct gattcacact cctttctgcg attgttgtaa tcgctgtctt
    15721 gaagggaatg ttttacattt gaattgatta aataaaattc atgaccattt aaattgagag
    15781 tatgataggt tttagaaaga gtatctgtat ttaatagaat acaatgatct ttttttccat
    15841 tggcgattat gtattgatcc ataataccgc atttcatacc aacgaaatta ttttcggcag
    15901 cctgtccgat gagagcaatt ttttctctag aaatagatag atgaaagata ctagaaagag
    15961 cataaccagt aacaacttcc aaggcggctg acgatgatag acccgcaccc tgtggtatat
    16021 ttccatcgat cgccatatca aaaccagtga ttgtaaaccc ttcttttttt aattcatcaa
    16081 ccacaccata gacgtaattt gcccataaat attttttatc ataactagga gtatctttac
    16141 ttacagattc cttgtattgt tcggagtata aattaatttt tccttggtca ttttttcgta
    16201 tggcaacaca aacttgaaaa tcaatcgcag caggaagaac aaatcctcct aaataatcga
    16261 catgctctcc aatcacattg attctagcgg gtgctcgaaa caatcgaatt gtatcattgt
    16321 tttcaccaaa cgcattgatt aaattttttt ttaaattaat aatagtatcc atcttaactc
    16381 gcaatgaaat taaacatatg ttgaaaaaaa catttactat gtctagataa atttattttg
    16441 tgtaagttgt aatttgatta gttataggag ataacatgaa tattaaaatc gattccaaat
    16501 tcttaaagtc atatttccct gaaagcttgc tttctaaaaa tttagagaag tccattttaa
    16561 aaaaagaatt attacataat aaaaaaggtc caggatcaga ttttcttgga tgggtagacc
    16621 taccttcaca aataaaagag agcgatttaa aaaatattca agatgttgca gatgaaatta
    16681 actcacactc agaatattta gtagttattg gaataggtgg ctcttattta ggagctagag
    16741 ctatgataga ggcaaataca aaaagctttc atatgttcga aaatcataaa aataaaaaga
    16801 ctaaggttct atatgcaggt catcatatcg attcagacta ccatgcagat cttttagact
    16861 ttttacaaga taaagaattt tctgtaaatg ttatatctaa atctggaaca actacggagc
    16921 cagctctcgc atttagaagc ctactatcgt tagtcgaaaa aaaatacgga aaagaaaata
    16981 tcaaaaaaag aatttttgca acgacagatg caaagaaggg agcactaaaa aaactctcag
    17041 atgaatatgg aattaaaacc tttgtaattc cagacgatgt aggcggaaga tactctgtgt
    17101 taacccctgt gggactcatc ccaattgcag catctggtat ttctatatca gaatttgtaa
    17161 aaggagcaag agagatgcaa gagtttttag ctaaggaaaa agatcctgtt aaaaatctat
    17221 cctgctatta tgctgctctc cgaaacactc tctatgaatc aggtaaaaaa attgagatca
    17281 tggtaaacta caatccaaag atgcactatt tcacggaatg gtggaaacaa ttatttggag
    17341 aaagtgaagg aaaagataaa agaggtattt ttcctgctgg agttgatttt actagcgatc
    17401 tacactcgat gggacaatac attcaagatg gagaaagaaa tttatttgaa acagtcttac
    17461 acattcaaga accaagtcga gatacaatca ttcaagaatt tgcaggtgat ccagatggat
    17521 taaattatat tgctggtaaa aaactttctt atattggaga acaagcgatt aatggaacaa
    17581 aattagctca ctatgaaggc ggtgtcccaa atattgacat atctattcca aatatcaatc
    17641 catctatctg cggagaactt gtttattttt atgaatatgc atgtggtgtt tctggatata
    17701 tgttaggagt aaatcctttt gatcaaccag gagttgagga ttataaaaac aatatgtttg
    17761 cacttctagg taaaaaaggc tatgaagaaa tgaaaatcaa agtggaaggt aaactcaaaa
    17821 gtctaatata aaaaacgaag aaggtggtaa aatgataaaa gcattagcaa aagaagattt
    17881 taaattttac acaacactcc gagtcagata tgctgaggta gatttgcagg gaatcgtatt
    17941 caatgcaaac taccttacat atatcgatac agctatcaca gaatacttca gagcgatagg
    18001 atacacctat ctagattttg taaagaaaca ttctatggat tttcacgtta tccgctctgt
    18061 tgtagaatac aaagctcctg ctcatttcga cgacgaatta gaaatttgtt taagaccaga
    18121 atatgaaggt gtaaaaattt tctggaagtt tgcaatattt cgaggaaatg aaatacttac
    18181 atttggagaa gttgtttacg ctagcttcga tttagaaact aagaagctta aaaaaattga
    18241 ttcaaatgta gtcacgactt tacaacttac agaaaactaa aaaatgtaga gtttttttct
    18301 acggataata tcgattatcc tctaatcatt caggcgaact tgaaataaaa aaagactttg
    18361 ggcaatttcc aaacccattt tccccgccct tagtctttat tggattacca ggagtatttc
    18421 cccattcata taaaacatac acggaagaaa ggtttttccc tttgcaattc atacgaatag
    18481 accctaccag atccggtaaa tttttctcta ctgcttcatt cacttcctgc ctagatatag
    18541 tcctattttt taaaggttca aaagcccctg aaatcaattt cgttttttct agtgcccatt
    18601 taaaataagt tactggctcc aatggagaac aaattccatg ttttacccac tcatgtctct
    18661 ctagacattt ttctgttccc gccatgtaat cactcatttt tttccacatc tcagaactat
    18721 ataagccttt gggagaaggg tatttacaga acttaccaag tttctcagat tgatttttac
    18781 attcagaaga gtcgcaaata ccctcagggt aaccttttgt ataattaggc cacagaccat
    18841 gtagttgcaa aatagacttt ttggggaagg tagcacaact aggatcgttg tttgccttag
    18901 aacaaaatgt ggctggaacc gttagctcca atcgatacaa tttctccttg ctagaatcta
    18961 attcgcaaga aggttcagca agaattgaca aaatagggat aacactaata actaaaaata
    19021 ttttaaaaag cattgcttca ctccaattta caagattcga aaagctacta tcgttctatc
    19081 atcttcctgt ggaacattca cagaaaaatc atcgagatct tctattagtt tattacaaat
    19141 aatttcaaca gattcatttt tatacttgcc tattgatttt ttaatattct caaactcaaa
    19201 aaaatctcca ttctgatttc tttgctcaaa cattccatct gttacataaa acaatatatc
    19261 acccttgaat aattctgttt taaataagtt ataattcgta tcttccctaa ttccgaacgc
    19321 acgaccacca gaaggcaaaa cgctaaattt atcatttcta aaaagtaggg gaggctcatg
    19381 accagcattg accgtataga taaaatatcg attttcttcc ttacttaaaa atgcgcaacc
    19441 tgccgtaaca aaaaaaccag gagcagtccc tttattagaa ccaatcaggt gataaagctg
    19501 agagttaaag tctctagcta agtcgtctat cgatttacaa atgccttgta tctgatcaag
    19561 caatgaaaca gatgctgtca taatcaatga gcttccaact cccttaccag atacatcacc
    19621 tataatcatc caaacttcat ttttgcctgt ttcataaatt ctaaaaaaat ccccacccaa
    19681 ttcctttgaa gaataactcg tagcataaat ttcataccaa tctgttctac tattaaatgg
    19741 taatagaaaa attccttgca ccttttgacc catttcgagt tctttttgca tgcttctatt
    19801 ttttaactct aattctcttt tttgttttgc aagttcatag gtgtgctcat agactttttc
    19861 ctgtaagttc ttgctataat cttctaagct tttgtttttt tctgctaagc tttcagtcat
    19921 tgcattaaaa gaagcagcaa gtctacctat ttcatttcta gtctttgtta tatacctaag
    19981 ctctagttcc cctccactaa ttcgttcagc aatgactgct agatctaata tgggtcgggt
    20041 aatttttctt gaatagaaag agaatataac aaaagcaaat aagacaatta taactgtgga
    20101 agctatcgtc gcatgacgaa tgatagaaaa ttcactcatc acttgttcat aaggaactac
    20161 ataaaccacc gcaagggact tatttataga gaaatgtcta aaaattaaga atacagaatc
    20221 tcctttttca tcattcgata tgataggtgg attgtttgac tcatctaaac tttgttcaga
    20281 gtttaaatgt tctgaaataa aattctgaac ctgtttatca atttccgaaa aatcagaact
    20341 aatcttaacg gtcattgtct ttgtatcaac taataaagtt tttccattgt tagaatcttt
    20401 ttcaaagaat ctttttacaa agagcgttag tttcacaccg acacccgcta caccaatctt
    20461 tttatttccg tcttgaatca atgtatttat atatgcatat gtattttctg aatcttctct
    20521 tgcttctgat ttggattttg aaatattcac ttcatagatt tggttcgaat tccagaactt
    20581 gaaaaaccat tcatcctcct tggaagaatt attgataatt ttcctttcaa tactaatgtt
    20641 ttttttatta ttcagatgat tctcaaaata ataatagcta ttcgttgaaa cattggaagc
    20701 gaagattcta gtgtattcca aaagctttgc aatattaatc attttggatt cagttgattt
    20761 ttttaatgtg gagtttgatt ctctgccttg aaaccactcg attaattctg ggtcagaagc
    20821 taaaagttga gaaactttca aagctttctc aatttcattt tctattttaa ctttctttag
    20881 ttcgattaac gtttttaagt cttctttaaa aatctttttc tcatagacta ccttagctaa
    20941 acgaaagctt agaagtccta ccattgcacc tgttagagcg attgcgattg cagagatagt
    21001 taatatttgt atgttaatcg actttctaaa ggtagctaat ggattcatac gatgaggcaa
    21061 tttgatgcaa ttctatttaa aatgcaagat tatttatcca ttttgtttta tttcctaaaa
    21121 aaacttccac atttgaaatg ctaacttgac ttttctgaat ggagtcccaa aatctaccta
    21181 tggaagattt ttctttaaat gatagtggtg aattatacat tcgttggaat ttaaatacaa
    21241 catatgcaaa actccaacta aatgaattaa taggtgcgct aatccaagag tatagacata
    21301 ctggtggagt ttatgatgca ttcattgaaa atgatggcaa attggtagga aatgaaaaaa
    21361 ttgtaatcat aagaaactta cgcgatgtaa ttcaattatc agtttgctta aaaaatcaat
    21421 tatctacgaa atacatcaaa aagagcacac aagaagaaaa gattatttgc aaattcattg
    21481 atgaatattc attccaattg acctataaat atacaaactc agaaatagag gatatctcct
    21541 ctgtaaaaat ttggtatgac aattatttca aaaaagaaat tctaaaattc acagatgatt
    21601 ttaaaaaagc actcgatgac aaggtaatca gcgaagaaga agctatcgaa ttagtaagga
    21661 ttctagatca tattatacta ggcgcagcaa ttctatttaa caaactcact tttgaaaatc
    21721 taaccaattg atccaaacct attaatctat tttttccttt tccgatacat ggaatgattt
    21781 cgcaagacag aggagatttt agattatgtc atataaaaaa atctttttaa catttttcag
    21841 tttgattacg attgaatctc ttcaatctgt tcctgaagta aaagtaattc caattcctgg
    21901 taatatcaac actgagatgc aagaatttgc tccctccttg accagtgatg gaaaaacaat
    21961 gtatttttat tccaaacgaa ataactcgaa atatactgat ttatataaat ccagtttgat
    22021 caatggtcga tggacaaacc ctatcgactt gagaggattg aattctgctt acgacgatca
    22081 aagtccattt atttccgagg atgaaaaatt tattgtgttc tcctccaatc gagatggctc
    22141 tatagaattt caacttccga gtggaaaaat aggtgtatcc agagatctct attattcaga
    22201 aaatataaat gggaaatggt ctaaaccatc aagcctatca gataaaatca atacagaaga
    22261 aatggaagaa aatccttttc ttcatgaaag cgatttttat tttacacgct atccctttgg
    22321 aaacccctca gaagcaaaaa tctggaaggc aaaaattatt ggaaatcatt tacaagaacc
    22381 agaagaatta ccttctccaa ttaatctaga aggaacatcc aatattgctg ctgtcatttc
    22441 aaaagatgga aaatatatct attttgcttc caaccgtccg ggtggttatg gaggatatga
    22501 tatttataga tctaaaatca acaaggatgg aagctatact gatcctgaaa atttaggtcc
    22561 ggaaatcaat acgcagggag atgaggcgta tatggtgatt gataaagcaa ataacgcttt
    22621 ttacttttgt agaaaaaatt tgaatgaaag ttatgatatt tatacagctt taatcttaaa
    22681 agacgaagaa gaagcgaagg tagcaaccaa agaaaatccg attcgggata agtctatcga
    22741 agaaacaaaa ataaatccac ccaaagacaa aatagaaatt ccgagcgtag tcgaattgcc
    22801 caaaaaagag attcctaaga aagacgacgt tacacaaaca ttaaaagaga aaaagaaact
    22861 taccttgaac aatgttttgt ttgatactaa ctcgagtgaa ttattatccg aatcatttcc
    22921 agtcctaaat caaattgtag actttttaaa agaacatcca gacactaaaa taaagatcac
    22981 tggacataca gatttaaccg gagatactac tttaaacaaa atactatcac tagagagggc
    23041 tgagtctgtt agaacttatc tacactccaa aggtatcgag agaaagagaa tgattgcaga
    23101 tggaaaaggc tcaacacaac ccttaatcaa taatgttgaa cctgaatcca atcgtgtaaa
    23161 tagaagaaca gaatttcaga tagtggacta atattgttag ccactaaaag tttatacttg
    23221 ccccaaagta gaagtattta ataatttgat gtcttggttt atcactgaca ctatcttccg
    23281 tggatctact cagtgcatat attgtatccg caaggtaata tcttatatat tcattcatta
    23341 gattttcatt ggttgtgata atcggaatat aatttgaatc tctatgtgca gcttttgtta
    23401 ttgttgttaa attatatccc aaaaataaat tcaacttctt gagtgagtag cctatttcac
    23461 cttctatttc atttccatac gtatcaactt tagataattt ggaatttatg tttcttgcat
    23521 atatcgagat tccatcatta cgaaagtcgg ttctttgatt attcacttct ccagacaagg
    23581 cttgaaatat ctttccagtt aatttaattc taaatgagtc aatgatctta aaatgaaacg
    23641 ttaaacctga ttgagcagaa taacttctag atgtctcact cacagaggaa taatttttag
    23701 gaaatgggta ggaattaaga tcagagttgc gattgtattt ttcagatatt tcacgaattc
    23761 ctaattcagg ctgaaccatt aaaaagttca ctaatgcatg atttcgaatc actgcgtatt
    23821 gtccctcgcg ccaattgtat tgaaatgtcc ccggataata ttcaaatgga acgttatttc
    23881 ctaaagcata atttcctgaa tagttcttag ctctaataga tacttcaaat gaccagtctc
    23941 ctgatttagt tgtataacta aatccaagtt ttcccaaagg agaaacttga tcattctttt
    24001 gattcagata ggaatagttt tctatattct cagtaagaaa tgcatctcgc tgaaattgtc
    24061 cacccgctgt atttagacta gcttgatttc taaacgcgac tgcactaagt aagtaatacc
    24121 cttctggact cgttggtcct ctactgtggg aatgagaagc atttgaagtg ccggacaacg
    24181 ttattttaaa acccggatta ataaactcag tagctggtat aaaaaatttt tgttttggtt
    24241 ttacatctga attctcttgg gctataactg aatttgctaa gattaatgtt atcaataaga
    24301 atataaactt catcgattac tctccgtata ctaaaacaca atcttcatag taaaatggat
    24361 agattgacat cgaagatcga tcaatgactg caatttttgt aataccagct ttatccatag
    24421 cctcatttat acttcctccc accccataat aaagtataaa gagatttcca aaatgactgc
    24481 tataagaaca ggattctcct acttttaata tttgattgga tgtgattgta tttttatttc
    24541 ctgctgacaa atgatgtttc gttgctccaa acatagcagc accaggagaa gtgatacaac
    24601 ttgttagaaa taaaattgct agaagaaata atatataaat tcttttcatt attccccctt
    24661 tacaagtgtg caataatttc catatacacc aaaaagattc atcgtcgaat gatcgatagt
    24721 tgctatacgt tttatcttat tttgcagagc aatactacct gcactcgcat ctccccacat
    24781 aaaaaagaaa aagatataat gtaggcaacc ctctcccttg tttgttgcag ggacatcatt
    24841 gtttggatta atttcaccag gaaattttgt ttgagtaaaa atcaaaccat gcacaggacc
    24901 attcgttttt tgcattatag gaagcataag gcattgattt aaaaaaagaa ttaaaacaaa
    24961 taataaaagt ttcaatttat tctcctgtaa ctattgtgca aaatcggtgg tatacaaaag
    25021 acaagatacc caattgttca taagacaccg ttgcaatctt agttatcttc ccatccttaa
    25081 cagctgattc gatggaagaa tctcctactg ctattagata aagcatagaa taggaacatt
    25141 gtttaccgac aagttttgat tccgcatttc caatgctacc cggagcttta acaaaatgaa
    25201 aaagccagcc acctttaaaa gtaagataat aaaagttatt atattcgggc atcgggtttg
    25261 tgttattagt ggaatttgtt acattccaat aattagctgc tgtgcaatta aataaactta
    25321 gtaagattac agaaataagt agtagggatt tccaagaagt catacagaca aggcatcgac
    25381 caaagctagc cttttcgata tcttttttta atagcaaata aattattaaa actttctgaa
    25441 ggtcagatcg taagcttggt ttttggaaat cttttaaagc gatggaagat aaacgctatc
    25501 cattcacttt cgatttgctc aagcctattc tcatttttca atgacaatag gacaagttat
    25561 caagactcac ctataaaaag atgagtcgta aaaatttatc tcatgtctct agttaaaatt
    25621 agaatttctt tatctgaaga tttgagagta gattgaatct tagcgggatc atcgaagcct
    25681 tgacttactt tagttaaaac atttactaaa tcagtaaatg aatatttatc accttccaat
    25741 ctatctacat aatataaggt attcgcccct ttttgcatcc agaaatctgc ccagaaataa
    25801 tagtctagag atattgaatc caaagaagat ttagggaaaa tactattgga gaaaagtata
    25861 gcattcggaa tcattcttaa atccactctt ctcctcagct tggaaaagga aattgtagaa
    25921 ttattctgac atctccattc aaaattttcg atcgaggaaa catctctatt acctaaatcc
    25981 tttgtagtcc agatatgcag tctttgatcg ttggaaaatg ttttagttcc ttcaaaatat
    26041 tcagctggca aatattgaat tccatttgta acaacttcat ttcctgaatc acaactaacg
    26101 gctacaggct gcaaatctcc ctctgatttc agattacctt tattaaatcg ataaaaaagt
    26161 ctgagattta gtaaagcatt gtttttcttc atggcttgat acaaatcaac acctaatgga
    26221 ttcataaata tctgaatgtt tcctgcttta tctagaaaag ggatattaga aaatatttta
    26281 ttatagtagc cagtagttct atagtcgatt gaattatgca gactgataga ccatttatct
    26341 ctagattctg ggttataaga aataacgtat attttctcat taaaagaaaa cgctgcgaca
    26401 aaaggatctc taatatcctt tagtgagtta acatcaaata aatcagaatc agaagacatt
    26461 ttggaagatt cagcttctct tagttttgag tatatacttc ttacataatg ataatcactg
    26521 ctaggtctaa aggatctgta atgcttcatc ctcttattaa catcgggcaa ttgtaaaatg
    26581 gtttttccta caatggagct agacttaaac ttcattttct gatagtgaaa taggaattca
    26641 atgagagatt gataattttt tgtgtaagta aaataattta cagcagtttt aaaaaaagct
    26701 tccgtatcaa atctagaatc tagaaaattt ttaacactta gaatatcatc taacggttct
    26761 tcatttcgca ttgaattcaa gagtgaacga aaggaaactg catggtttcc tctttctccg
    26821 ccctgcactc tcgcaatcag cattccagag gatctcatcc attcagaagt gaaggcagga
    26881 tctgaaccac ctaagattcc ataactttta gtaaattgaa cagttgcctt tcgatacttc
    26941 ttactaataa tgctacaata tccattcata atggttgcgg catattcaac tctcttccaa
    27001 ccttttcctt gtgcgatata catgatatct tccgaaattt tctcagccat ttccggtttt
    27061 tcagccatca tgatttgtgc gattcgaaga cgaagaaata aactctcttc cgtaatcgta
    27121 ttatcagtat ttaacttatt cagtgctcta acagcattta cagcagattt tgatttccaa
    27181 agagaaagag aaatgagtcc tcttgctcca ttgatagaaa ttgaatcatc ataaaaagga
    27241 ttcttgatcg atttattcca ttcctttaaa ttcaattcaa gataatagtt catagactgt
    27301 ttataatctt ttcgaagaaa ggcgattgca cctaacttca aaaaaatagg attcttatta
    27361 gaggtaatag cagaagtttt ttttgaagaa tagctcaatg cctcctcagc tgatgttaaa
    27421 tcatcattta acatttgata aaaagacaat ctttcatgct cagtaggatc aaaagcgcca
    27481 ctaatctttt gaagattaac aaccatcttt tgaaaatgaa ctgcttcata cgcaaatcct
    27541 agatatgcaa gtttactagg catttcaaag gtgaattttt ctaggaaact aacgtgtgcc
    27601 agttcatatt cttcaaagaa tggagctaaa agtctatcta gatttaggta atctctatgc
    27661 ggataatcac tcgtttccgc taaatgctct aaatgaacag atactttaat taacctacag
    27721 aatgaataca taggtttgct tctactacaa tttaaattct taattacatc ttttgtatca
    27781 gcaggaattg atattttttt taaattactc ttaactacct tcgctatact ttgatgaaga
    27841 ggattttctt ctttagcaat gatttcatct aacttaaata aggcatcatt ggtctctgcc
    27901 ttttcaattt tccaaaaaac aagtattaga ttagcaataa attcataact attactccta
    27961 tcgcttttta gtaaattcgc taaatttgcc aacccatctt tatctccgcg agatatataa
    28021 tactctgcta tataagtcgc atactgttgt atttgatctg aatttaactt ttgaggaagg
    28081 tatcgaatcg tgtctcttgt atcaaaaaca gacagatcat ttatataaag aacggagtga
    28141 ttatttccaa tctcccaacc agtataagga gcagaaaaga tgtttgtgac aaataaaaaa
    28201 aaagtaaaaa gtgcaaatct aaatttcata taataagtat tctcatacag ccatagctat
    28261 tgcaaattaa tgtctatctt gtccgactct tcctgactag aatcttcact ttttcggaat
    28321 cggaaggata tcatgtccac gatgtgttct gtataatatc tgtcattcgt ataagtttga
    28381 gcaatttcat ataaatgttt ttcttcagca gcattcaatt ttctctccat agataaattg
    28441 aaaagggtta ggaatcctaa ggcagacaaa gctttattct ctgattttaa ataggtgaag
    28501 tattcttctg agtttatatt tgcaaagctt ggaattaaag ttgaaagcaa ttcgattgcg
    28561 ccattaggag tgtcttcttt aaaattctga atataccatt ttaccagagc atatgccttt
    28621 tcatttccaa tatcaatcag tagataaaat atacccacca ctagattcgt tttttcgttc
    28681 caaatattgc tatctttaat taaatcgcca acgatacttt taaaaagaaa ccaaattgct
    28741 ttttcatctt ctaaagcctt ttgttttaag tcttgcagaa gactctgaaa atggaaggaa
    28801 ccatccacct ctgccaattg ctttagatat tcttctactt tttcattaat cgaatcactc
    28861 attacttatt aggtcctctt tgcgcttctc ttaattaata taattctttt gatttgcaac
    28921 aaaaaatctt tccgtctcaa tagtcttata gaattcgatg caaaaactga gatccttcct
    28981 ttttactgct cacctagatt tgcgctattt tcattgtcca acaaaacatt gattgagttt
    29041 gcactaaaat ttgcaaattg gtctaacagg attttttcat aatatcagat tctaaataac
    29101 taatgaaatc gatccgtgcc caatttaggc aaaatatact tttgtcttcg aatcaaaaac
    29161 atatcatcta aaacgataat attctcggtg ttttgtattt ccccgatttt tgctggaatg
    29221 atattgaacc caatcacttt agaaatactt ttatcctgta ataaaaagtc agattggtag
    29281 attccatttt gaataggtaa tttaatttgt ttccatccat caaaatcaag atgtgtaatc
    29341 aaaatagtgt gaaactgaaa tttgttatcc attaaaatca aaaacaaatc acttctaatt
    29401 ttatttgagt atatgttaaa ctcaatctcg atgatatgat cagaaattaa taatggtttt
    29461 ttaaaaatta tttcaaatgg attttttttc tgacctacta aattacgaat caaaagactt
    29521 gtgtttgatt gtaaatcagg cgatgtaagt gtttttgaaa attttatttc cggtaattca
    29581 cttgtatttg cagatacttt tacatttaaa tcagataagg aaatactttc aaaatcctga
    29641 atgcaaattt tctctttaaa aataaattct tctttcgctt gagcgaataa agaaaataga
    29701 aaaagatttt gtataaagag aatgtatcca attcgtataa tttgagtaaa cattgtatga
    29761 ttacaataga atgaaactag atttattttc aagagcaaat gtgttttttt aaaatgaata
    29821 ttattcgtat atttgtcaat ccttgacata cttgcttgca ttcgaatata tggtaaatta
    29881 aacagttaaa ttacatcata ctaggagact ttagatgaac aacatcatta aactctttct
    29941 catgcttagc tttactatcg cattagttgc acaatccggt attaaaccac ttccaaacga
    30001 tggaaaaatg gatagagctg agaaagctat tgaaacaata cctacaaatt cagttgatag
    30061 cctaaaaact gaatctaaga caacagttac tacaacaact acgaccacaa caactgctgc
    30121 acctgcacca gtaaattctg ctcctgttgt aactcaacca ccagtttcaa atgcaagagc
    30181 aatcgatcca aatttaggta atacacctgc attatatatt aattcaagaa cagcttttga
    30241 attagtaaca ggagatgact taagtggagt tgattacatc gaatacaaaa ttaatgatgg
    30301 agaatactca agatacactg ttccaatttc tctaagcaaa gaaggacctg ctacaattac
    30361 ttacaaagca accgataaag ttggaaacaa agaaacagca aaagtaatta acttgattgt
    30421 agataatact gctccaaccg tttctgtaaa agtatcagaa cctcttttta caaatggcaa
    30481 cacacaatat tcttctccaa aaaatacata tcaattaatc gctgaagata agtcttctgg
    30541 tgttaaacaa attgtttatt ctgttgataa cgaaccaaaa caagattaca aaaacgagcc
    30601 aatcaaacta gaaaagcctg gtttccatgt aattaatttt tatgctattg acaatgcagg
    30661 taacgttagt caagaaaata gctacattgt aaacattgat ggaattaaac caacagttga
    30721 tataaaagaa tctatccctt acattgtaat tgcaaacaaa gtttatgcaa aaaaagaaac
    30781 tgttttcaca attacagcag ctgatggaca aagtggaatt aaacaagttc tagtaaaaac
    30841 tgatggtgct cctgatttcg ttccttacgt tgagcctctc acattcggaa catctggtga
    30901 acatatcatt gaagcgaaag cgattgacaa tgttggaaat gaaagtgata ttaaaagagt
    30961 agtattctct aacgatatca aagctccaac aactgttatc aaagctgtat ctaattaatt
    31021 cttccgatca gacgcgctaa aaaaaagcgc gtcttccatt ttttttcaaa aaaccccgca
    31081 cacaaaacaa atttttctgc cctacattag tatgacagaa aaaactttta caaaactaat
    31141 tcaccttttc tatccactgg attgtagtat atgtggagac acagacttac tagcatctaa
    31201 gatcagtatt tgcagaaaat gctataggtc cgaaacaaag agccaaaatc caaatctaga
    31261 aaacctctgc gagaaatgta aaacaaaaat aaacgcaagc gaatgcccct attgcaaatc
    31321 tagaaatgtc ttctttaatc aactctacta tttaagagag aaaaacaact ttgagacaga
    31381 gttaatacgt aaaataaaat ttggaaataa tccttatcta acaaactatt tcagaatagg
    31441 tcttggaaaa cgaatcaaaa tcttaaagaa taattcatat aaaaacatca ttcaaattcc
    31501 atccaatcag aagacattga gaactagacc attcgcggta agtaaaccga tttctgatta
    31561 tttgatgaag aaattaaaac tggaagtgat aaaaccaatt gtgaaaactt caagtgaatt
    31621 gcagtcgggt aaaagtttta gagatcgatt ccttcatgct caaacagctt ttagtgtcat
    31681 agctaagtat aaaaatcaac tcaatggaaa ttatttattg gttgatgatg tttttacgac
    31741 aggcgctaca gtgaacgaaa tatctaaact cttattaatc aatggagcaa agtcagtaga
    31801 tattttagtt cttgcaaaag ggaaagttta atttcactct aagaatttaa actcgtcttt
    31861 tactgcaaac ttactcattt tctggttgta ttttccatct tgcgcaatca tccatacatt
    31921 tcccttcagc gctccatttt ttttatactg gtattgtatg taaaaattag taattttttt
    31981 ccaaaaatct ttttgttctg catttacctc tttcaaagaa cactcacctg gaacaacttc
    32041 tatgcttgca taggtcgaac taacctttct aggatttgta aaagaaacaa tcgaatagtc
    32101 gcagaagtct ttgggattcg ttaaatatgg tgtaactcgt agtttatatg tgttcaaatc
    32161 gggatccttc atgactagta gcgatccctt tttattttct gtatttctta cagagcttgg
    32221 cagtttaaac aaaccataaa gaacctcatt ctgttgaaag tcatgtaaat cttttttaaa
    32281 atctgtcgga attacatcgg tcgaacttgg agttattatt ttggattcct ttttggtcgg
    32341 aactccgttg ctctctgtca caccatcctt acttataaat ataggagtct ctgcacttac
    32401 ccactgatct tgcggaagcc gtttcgttag ttttttaagc caagccatat tagagggatt
    32461 cattcccttc aaaactggct ttgaccaata cttagattta tgtaatttct taatccgatt
    32521 tctagaagca attccaatga tgatgtcttt accaattcga tcgcctgcta agaattcata
    32581 gtctttcttt tcatcaggaa ttctaatttc ctcgttgaaa accatattct cttgctcctc
    32641 ctcatttgga taaagcaagt taatatttcc atcactttgt agtgttaaga tataaaggta
    32701 agacgcttgt tttaatttga tgtttatttt aactgactgg cctggtcgat agattttaga
    32761 atttgtctct atagaaattc ccatatcatt ggaagtagga gaattagaaa ataaaaacaa
    32821 aaagggaatt ataaagagaa tccgtttcat gccatgagat ttttatagtt ttttattttg
    32881 tcaatcaaga tagttggaaa aaaaaatgac agtagaatta tgtttaagaa attgtaatgc
    32941 atagatgcta gaagataaaa atactcctaa agcctttgaa ttatttcgaa tctcaataat
    33001 tcagttaaca attttaagtt tcagttttat tttattatcg attttctccc aattagcgat
    33061 ggcaacagtc gcatatcaaa atcgattgcc ggaaaattta ttggacttaa gtgaagaaga
    33121 aagaaccact tttcaaaata aaattgcaaa agagtttgaa tcattagcga agaatcaccc
    33181 agagaaaatt gcagatgaat ttatcgaagc aaccacaaat acaacaccaa gccttttatt
    33241 gatacagaat attctttggt ttgtaagctt ccttatacca gcctattttg ttttcactcg
    33301 atttctaaaa gatgatgttc agtgtttaaa cgatgaattg agtttcacac atattaatcg
    33361 aggtttaatg gctggaatca gtgtatttat aatactcatg ttagtatcct tagtttttta
    33421 tgttttcaat tttaagccca aagtaaacca cttccagaca aaacttttta caaatataaa
    33481 aggaaatttc tatttattag cttggtgcat ctatactgtc ggaattataa caggtataat
    33541 ggaagagttt ctatttagag gtctgatgtt aaatcatttt gtaaaaaaag gttatgcaaa
    33601 ggaagggttg atgattactt cctttatttt tggaattatg catttttctt ttgatgcctc
    33661 ccctgtaata cctgcgatcc ttacatttgt aggagttcta tttggttcca tctatttaca
    33721 atctagaaat atatggacgg ttatggcaac tcacgcatgt tacaattctc ttggcttgat
    33781 aactgcctat tttctagggg acaaactttt atgaaaaatt ttttacaaag agtatatatt
    33841 gtttttttac tgagtgttcc attttcacct ttattttctt ctgaatcttt tgaaagcagt
    33901 gcaagtgtag aagatgcaaa tttaaaagtt gtaaagctat taaataaaat tacggacaat
    33961 tccatacgct caatggagtc cacaggcggc tttaattatc gttacaaaag tggtctaata
    34021 agccctttta gatttaacgt ttatgttgga aaaatcagca agaaatcgga agactccatt
    34081 ataagaattg agtctactaa aaacggagaa gcaaaattac taaaaaccat tttggaagta
    34141 gagctaaata aaagtgctgc tgactataaa tttgaacaag tcataaatcc aaagtatcat
    34201 atagtatctc aatcattaaa tctaatcact cccgcagcaa gtgtccttta taattcttac
    34261 aattctccat tttacacaaa ctcggataca atcctgaaag ctggaaccta tgtgttaata
    34321 gatttattta tagtcgctct agcagcagtt tatatcaata atagtaaagt tggaaataaa
    34381 aacatttatg acgatttact aaataaaaaa ggaccaggaa aagactttag agattatcca
    34441 caaagagaat tcatactcgc tgcactcctt ataccaagaa tatacagaag tatagaagca
    34501 tttcatgata cagcagctca aaacagaatt gcagaacttt cttataccta tcgtttttaa
    34561 acaattaatc ctaacctgtc caaagagtat ataaattcct tacattttta cgactattat
    34621 ttatataaca aaaatttatg taggaattta gaaagtgatt tatggtatcc accctttatt
    34681 ggcgaatatt catatttgaa aggatacgat ccatttcatc tatactattg taagatatta
    34741 caatttttcc cttacctact ttagcgttat gcgaaatttt aaccttagca gataatttat
    34801 ttctaatttt cgtttctaca ttcataatag taggatctgt attatctctc ttagtgctaa
    34861 gagtcgtttt tgtttcccag agactagcaa catactcttc tacatctcga acggttaaat
    34921 ttttatctac gatttgttgt gcataggttt ccaatttctt tttgtcacca atcgatagaa
    34981 gcggccgtgc atgaccttca gataattttc gagttttaat taattctttt actgaatcag
    35041 gtaattgcag taaacgaatc atattggaaa cagtagagcg atttttccca acacgcgctg
    35101 caacttccgt aattttcata gaaagctttt ctgtaagctg ttgataggcg agcgcttctt
    35161 cgattgggtt taaatcttct ctttggatat tttcaatgat tgcaatttcc agagtctcct
    35221 tttcgctata gttccgaacg atcgcaggaa tcttatgaaa gcctgcttct ttacaagctc
    35281 tataccttct ttctccagaa actaattcat atccgtcatt cgttttagta acgactatcg
    35341 gttgaatcaa tccatgttgt ttaattgtct ctgcaagttc ggaaatagat tctgcagaga
    35401 aagtttttct cggctggttt ggatttactt ttatttccga taatttgatt tctctcattg
    35461 gagaattagt agaagtatcc gaggactccg atgaacgacc agaggagcca attaaatttc
    35521 caagaccttt gcctaatact tttggtttca taacatcctc tatttatttg ctgcgatcac
    35581 ttcatcgcaa agacttttat aactttgggc gccaactcca tctggatcat agaaattaat
    35641 cggttttcca aacgaaggcg cttctcctag ttttatgttt ctaggaatca tggatttata
    35701 aactttctct ttgaaatact ctcgaacatc attcgcaact tgatttgcta aatttgttct
    35761 cttgtcatac atcgttaaaa caactccctc taattcaaga gatgagttta aattttcttg
    35821 gacgagagaa ataatcttca ttaattgagt aagaccttct aaggcaaaat attcagtttg
    35881 caatgtaatc atcacactat ctgaagcgca taacgcattt actgtgagca caccaaggga
    35941 aggtggacaa tcgataacga taaaatcaaa tcttgctcga atcggagcca atgcatctct
    36001 gagcctaaat tcttttctat cttctgacat taaatcaatc tctgctccac tcaaattaat
    36061 attcgatgga atgatagata gattttcgat accagttttt tgaattgcct cattcgcaga
    36121 aacatctcca attaaaactt cataagaggt attttcaatt tgattgatat ccattccaag
    36181 cccagatcca gcgttgccct gtggatctac atcaatcaat aaaactttct taccactcgc
    36241 agcaagataa gaacatagat tgatggaagt agtagttttt ccaaccccac ctttctgatt
    36301 actaattgaa attatttttc ccatgatatc ctctttatct ctttactaat cgattcccaa
    36361 ttccttggaa aacctttgtt tggattcgct tcctttttca atatttttat atgacgattt
    36421 cccaaaaact ctaattcatt taaatctaaa tctttttcaa tgatcattcc taaattctgc
    36481 aagagagttt tttcaaaatt tgtatcgtag actcttttag ctaaaaaagg aatgaagtag
    36541 gaattctgct ttaaaatttt ataaaccatt tcacaagacc aagggtaacg aatagccgat
    36601 ctcattacga ctaaatcaaa ttgttttttt ataccttcca tcctatcata taaaaaggaa
    36661 actttagagt cgtagttttg ttctttataa aacttttcca aaagagaaag tctgcgtgat
    36721 tgcgaatcaa tcagtgtgac tttcggggaa ttttgcagac aagaaaataa atatccaggt
    36781 aaaccgggac ctgttcccac atccgcaact tcatcacttg ctttaatata cccagaagaa
    36841 ttgattacaa aaatatgaaa catcgattca ataacatgcc tatccaaaaa aaactcagaa
    36901 tcagatttgg aaaagaatcc tccgatctca ttgtagtcgc gaagaaagtt tgaatactca
    36961 atcactttgg atagatgaaa tatttctaag atttgttttc cagtttttgg aaataaagta
    37021 agaattctgt caggtaaaat taattcgctc attggatatt gattcactta atggttcaga
    37081 tttagattaa tttcagatag taaattaaca caagtcaaat tggttctatt gtaaaatgtt
    37141 cttatactac aagtaaagtt tcatattcga gagaatgaag gttctatcta tcatttcctc
    37201 ttttacattt gttgaataaa ttagattgag ttgccaatta gaaagaattt taacttctta
    37261 caatatccct tttacggttt tataatagca gataaatttc tatttaaatt gggatgagtt
    37321 tccatttttt gttttaaacg ctttctaaat ttcatttttc taaatggaac caatttaatt
    37381 tttgagggtc ttattctgca aataaggaga taagaatgaa aaaaatattt aaagtatttt
    37441 cccttctcat gttggtattg gttgcgtttg taaactgccg tcatcactca ttggcagaca
    37501 gagcagattg gattgtaaaa agaatttcta gtgagctaga tctcaatgat aagcaaaaga
    37561 cagaattgca gagaatcaaa aaagagattc tagacaaaaa gaaagaactc gatctaaaaa
    37621 tgtttcccga tgatatagta gatcaaatca gacaaacaca aatcgatgaa gcaaaggtaa
    37681 acaaatcctt tgaagtaaat ggagcaaaaa gagatcaaat gagagttttt atgactaaga
    37741 aattcattga gtttcatgcg atccttacac cagaacaaag aaataagtta gctgataaga
    37801 ttggggaatt actaaaaaaa cacaaccatg actaattctg attttacgtt aatagtaaat
    37861 tcgactaaat cagttgttct ctctgcgata gaaaaaaatc tcgcagagag attctattat
    37921 gcaattgatg atgtagtgca agaaacatat cttcgtgcct ataaatctct tacaaaaggg
    37981 aacttccgat acgaggcaaa gatttcaact tggctttaca caattgcaag aaatgaatct
    38041 ctcagaatga acgacaaatt gattcgagaa gaaaagaaaa tagaaaaagc aactagccaa
    38101 ataaaagaag agcgacccat tgaagaggaa gttgaagaaa atccaaagaa ggttaaccta
    38161 cttgattact taaaaatgat tcccgataaa tacaaagaga tcctagaaca ctatatcaat
    38221 ggactatcag aaaaagaaat tgcagatagg ttatcaatta aacaaggaac tgtaaagtcg
    38281 agaacatcac gaggaaaaga attcctacga aagattattt taatgggaga aagaaatgaa
    38341 taaaccaaat cgaaaagaag aaataacatt tagattgaat agtaatgaat gggacaaaga
    38401 tatcgcagca aaagtaacaa acaagtatca aagaaaagta agaagaaatc gtgtgattgc
    38461 aacttccgca ttcctactac tcatgggagt ctctactttc ataactttct tctatcaaaa
    38521 tccagttgag acagaatata tgaacttaca agcgtttcag tttttattct ctgacgattt
    38581 gtttcctatt tttgatgcat tttatcaaga agaaagtatt tctttttttg cagattccat
    38641 attgtaaata gaaacaactc aaaccataaa taatatttat aaaaagtatc acaacgttta
    38701 ctactaggtt tgaagtgata cttttctatt ctatttctat ctaagaaatc aattcaatcg
    38761 agaagataaa ttataaccaa aaccttgatt gcaaaaatat atacgaaacg cctaagaaaa
    38821 gttcctataa gaatttaatt aaactaacga ttggtatacc ccacatacaa acgtgcgagt
    38881 aaaataagaa atgaattttg atgttcggat taaacagata aacacattct cttgtctaga
    38941 gatcgctctc tttgagaaga atcatttata taaaaagaac ttcttttgag ggcaatcaaa
    39001 atgccccgac taaactaata aacaaaatcg tcgatacttc tattgatgaa aaccttcctt
    39061 cttttctact ttttagttct ttcaatttta tatgcttaca ctccaggtaa gtggtcttac
    39121 ttagatcggt ttgtggttgg aaatgaaaag ttcggtccag tttctgaaat agtaaaaaac
    39181 aggacagggc aaaccattta ttctgctatg tatgaataca atgtagaagg aaaattgatt
    39241 aaagagaaat ttctaaatgc aaaaggagaa aatgacggag agattgttta cacgtatgat
    39301 tctggaaaag taaaaacgga agaagtattc tcaccagaca aacaatcaca agaaaagaaa
    39361 acattctctt acgcatcctc cggcaacttg aaagaaatca ttatcacctc tgcttctggt
    39421 aaaggagttt tacgacacaa gataaattca attcaagatg gattcgttct agattgtgaa
    39481 accaaatggg aggaagaagg agctcaagaa tctttttcat ctaaaaaaga tgtcttagat
    39541 gaaacaatcc tcaaccaaga aatattcgat gacaaaaaga aatccattgg tttcataaaa
    39601 tactattacg ataaaaaagg gcgaatcgaa aagagggaaa acatgcaagg caattccaaa
    39661 agaatgcaac ttctaaccta cgatgagtca gggaaattga ttagtttttc gtttcacgtg
    39721 aaacaggatg aaaactgggt tttagttaag actcattatt tgacttataa ttcaaataac
    39781 acacaataac aatatcttcg ttctttcgaa tttaattcga agagtctagg attatatcat
    39841 tgttttttgt ttgagtgtta ccttttaata atttagcaaa cttttttgca atcacctcaa
    39901 ttacaatcgt gaacgcgcct ccataattca tccctattaa taattgatag accatttttt
    39961 ttagagccaa gtcaaaaggg agacgatatt ctttccagtc agcttgaaac tcatattcta
    40021 atttctgttt tcgatctgac tgattttgct tttgaggttt gcgattgatt tgaccaagag
    40081 ttccattgaa ccgagtgata gattcggtat gtgtaaagtt taaattcaaa tctattttgt
    40141 tttgcagaaa cccaattggt agaatatcta aattaattaa tttaccccaa gtcactcctt
    40201 ttacttgtaa ctgaccagtt ccattcttac aagttgagat cacatcgcct ttgtcaattt
    40261 tgcagtaagc atattcagaa ttaaaaataa gttgaaacgg atttcctaga tcaatcaaag
    40321 catctgcaat ctctatattt cgaatgtcag ttttataaat aggaaaaaca gacatatcct
    40381 caatttcaat attaccattt gtaatgtatc cagaatgaat gattgctttt ccttctctag
    40441 gcatatattt aattttctcg atggaaggga tatgattgat atacttcaca tctaagtttt
    40501 taatcctaaa ttgataaatg ctcaacctac ctgcaaataa agaaaacggg tttagaaaga
    40561 aaaagattct atctacttta attttaacaa catcggtttt atttacaccg cttgttttaa
    40621 tttccaaatt tttagcataa aagaaaaata taggaaggaa aaatccaaat agatcacatt
    40681 tacaaatcaa atattttcta aagattcggt tcactagaag agtaaataaa ggagcagtta
    40741 aaaacaactg taagatcaaa tatattaaga atgtgatttt gatattttcc atattcagtc
    40801 aaactagatt caaatcagtt taagtctcta aaacttgctt ctatctaaaa atataaatcc
    40861 ttcgaatctc ttttttatgt ctctataaaa agatacgaat cattttcaaa ttagaagtta
    40921 ccttttgtat tcggaataag attaccttac ccgaaagaaa gaaaagtata aaacaaatcc
    40981 aaataaaaat atattagcaa ttcatctttc actcctgcac tctatcaaat taggtttaat
    41041 ccaaatctta ttcaaaagac attccattca gcagagtaaa ggagaaggaa gagaggaatg
    41101 gtataaatcg aattgagaac agtccaaact caactcaaaa ccgatattca atacttgcta
    41161 tctatttcaa aattgattct tttattttaa ttgaaataaa aatagaaact gaaagattac
    41221 ccttaaagtt aaatcgaaat ttacaaattg gtttttaatt atgtttcata aaacgtttcg
    41281 ttaagcttga ttcaaaaaaa agctatacat agacttttta ttaccgatgt ttcataaaaa
    41341 gattcatctg ctgttagaag catttagaat aatgatttgg taaaagacat tacctataaa
    41401 aacgggaagc tgattaatag cccttggaaa aatatttgaa agacatagaa gtgaatcagg
    41461 aagtatttga aataaaaaac tccgaaatag atgtcactag tatcatggca gacattgaat
    41521 cgaatctaaa acgaagagcc atcgataaaa cagaaataga aagaatcaaa caactgaaaa
    41581 tttctgctga aactcctgta ggtcatagac cttttgatcc atctcatgtt gcgaatcttt
    41641 tcgaaaaagg catctctcct ccaagattct caaacccaaa gtattggttt gttaaaggtc
    41701 cactcaagtg ggcaattcat aagtttacag atttctattc ccttgtagat aaaaaacttt
    41761 ctgaaaatag aatcaaagca ttttactcgg tcgtgtatga attaatacta cttcgaacac
    41821 aatatgaaag attagaaaga aaatttaatg atctttataa aattacatta aactcagaaa
    41881 cggaaaataa caactatcaa tacagttcca tgttttataa tcatgatgag ttactatctg
    41941 aaaagttttc aaatgaaaat gttcggattg gaaatctaat ttcaaaagaa agtaaaactc
    42001 tcatcttact ccctgctgca gataattttt taaatttatt aagatggaac aaaatagatt
    42061 tccatttaat cacagataag aaacaacaat tggaatacat tcaaaaacaa ttaacaaaga
    42121 atatagaata cgttgaatca attcaaagct ttactaattt taaaaattat aaaaatattt
    42181 taatccattc aaatctttgc ttacttaacc caaaaaccat tgagagaatt attcaaaaca
    42241 ttttagaaca agcagataaa aacacacaag ttctactcca attctcaaat catactcttc
    42301 aaacgatttc accatttcgt gaaaactttc aaacatacat cgaagtggat catttggaaa
    42361 cctacctaag aaatttaggt tttatcaata tttgtaaaca ctctgtagaa aaagaagaaa
    42421 tgcaactcat cactttttat acaaaatgag aatctttcag catgtagatg aattcaatga
    42481 gcgagatgga atcggaaatg atatatctgg atttgattcc atttttaaag atctccattt
    42541 agaatcgaat atcatttgta ggatcaataa ctccaaacag aaaacggata tttattcacc
    42601 agaagacaaa ttagtttttt ccaaaaacga tgtgcatatt cttcactatg gaggcactgg
    42661 atacccaata gattttttcg caaacctacc agggaaaaaa attttaagat ttcataacat
    42721 tactcctgcg tattttttta agccttattt agattctagt atctatgaat catttgagaa
    42781 aaatgagtct atttctttat tcgaattata ctctttgaga aaaaatattc attttctttg
    42841 gagtgattca aaattcaatc aagatgaatt ttttaaattt acaggtaaga cgaataaatt
    42901 cattgcagaa gttttaccgg ttactcatcg ttatccgctc gcaacagcaa agagtgaaaa
    42961 caactttaca atagggttcg taggaagatt agtcccaaat aaaaagattg aggatttata
    43021 cttcacttta ttttatttaa agaaaataaa tccaaaatat cggcttattt tattcggtaa
    43081 aagaaatcag atttttaaaa aatactttca aaaactggat tcaattgcag aagaattaaa
    43141 tctattagat tctatagaat taaatgaaaa cttagatgat gcaaatctaa gagataagtt
    43201 ttcaaaacta agtgtatacg tttcaatgag tgagcacgaa ggttttggtg ttcctttatt
    43261 ggaagctatg tccttaaata tcccagtcat cgcctactct tctactgctg ttacagaatt
    43321 agttgagaat gcaggacttc tattttcaac taaagacttc ccaaaaatag cagaactaat
    43381 tgatacctta cattcaaatc caaatataaa agatagactt cttcaaatgc aaaagaaaag
    43441 aattcaaata atttctaact tttcatacaa agaaaaaatt caaaacctaa ttaaaatcta
    43501 gaatactttt aagaatgaca attcatattc tatctaaaat ctgaattctt aatgagatac
    43561 cctattaaaa gaatcattca attttcagct ggatttaatc cgggtgatgc tatcagtaat
    43621 gaaatgataa ttttaaagtc tttttttcaa gaaattggct atgatgggga aatcttttcg
    43681 gaaaacatcg gatctaaatc caatcgtctt gctgcaaagt ataaatccta tactcctaga
    43741 aaaggtgatt gcatcattta ccatcactcg atccattctt caattctaga atttgtttta
    43801 aatctagatt ccccaaaagt attaatctat cacaatgtaa ctcctagtta tttctttgag
    43861 ccttatgatt taaaactaac gtattattta aaaaagggaa gagaagagtt gtcagagtta
    43921 ttaggaaaat tccaacttta ttttgcagat tcgaatttca ataaacagga attggttgat
    43981 ctgggtttcg agaatgtcca tgttcttcca atcatctatg actttagcag attaccaaaa
    44041 aaagaaataa aaaaaagtaa atcgactaaa gatataatct ttgttggaag aattgcccct
    44101 aataaaaaac aagatgatat aataaaggta gctaaggttc taaaagaata cttcttgagt
    44161 gattttcgca ttcacctagt tggatattgc tctaaggaac tggaactcta tagagatgaa
    44221 ttaaaatctc tcattgattt atttgatctc tcagatcatg tcttcttttc agattttata
    44281 aatgatgatc tactcgctga atactatcaa aacgcggacc tatttttatg tatgagcgaa
    44341 catgaaggct tttgtgttcc actcattgaa tctatgtatt atgaggttcc tatattggct
    44401 tttgatgcgg gagcagtgag agatacttta gatggagcag gcattctttt taaaactaaa
    44461 gactttccaa ctatttgtga atgtattata aagattcttt ccgataaaga ttttagagat
    44521 aaaattgtag aaggtcaaag agagcgactc actcgtttta tgcaaagcaa tcacactaga
    44581 atcatcggag atgcaattaa acatattcaa atatgagaaa gtattttatt aagcgcctct
    44641 tattattatt tccaactgtc cttggaatta cattcgttgt atttttaatt tctcatatgg
    44701 cacctggtgg tccttttgaa tctgagatcg caaagtggcg tggacttgca aatgaaagtg
    44761 gaggaagtca aaaacaaatc tcagaagaag aaattcaact attaaaagaa agacttcacc
    44821 ttgataaacc ggcccctatt gcatatttat attggttaaa aaagattgta gtttttgatt
    44881 taggtgaatc tagaattaat tataaaaaag tttccgatct aattgtagag aagcttccaa
    44941 tatcattgac gttcggactt agtggttttt ttatttctta tattatttgt attccactgg
    45001 gtatctacaa agctttgcat catggggaag gatttgatct agcaactagc tttattattt
    45061 tctttacata ctccattcca atttttgctc tagcactttt acttttatac tttttagcat
    45121 caggtgaatt tttctctcta ttccctttgg gtcatgaagt ttccgatcat tatgaatctt
    45181 atgatacttg gggacgatta atcgataggc ttcatcatat gtttcttcct gttctgtgct
    45241 atatctttca ttcttttgct gtgcaaacaa tactaatgaa gaattcactc ttagaggaaa
    45301 ttagtaaaga ttatgtaagg actgcggtat caaaaggaat gagctttaag gatgcagtct
    45361 ttaaacatgc ttttagaaat tcactgattc ctattgcaac aggttttgga agtaatctca
    45421 gtttaatctt tgctggttct ctcattattg aattggtctt tagtattgat ggaatgggac
    45481 ttctcagttt taatgcagtt cgcgaaagag atactgattt aatgatgggt ttactacttg
    45541 tgcaaagtat tttatcatta cttggaaata tactgtctga tttttgttat gttctgattg
    45601 atcctagaat acggtttgaa tgatgctaaa tccaaataca aaaaagagat tattaaaatt
    45661 taaaaataat agaagagcct attactcttt tctcttccta cttataacgt atatcgtttc
    45721 tttatttgcg ccaattcttg taaatgatac tcctatctta gtttattata acgaatcttt
    45781 ttattttccc atttttaaat tctatccaga aaaaaccttt ggtggaaaat ctgagacggt
    45841 tgcgaattat aaaaaattaa acaaaacaga agcattccaa tcaaataaaa atttcatgct
    45901 ttttcctctc atttcctacg gctacaatga aagcaatatg gcagatctcc cttttggagt
    45961 ttcccctccc acagcacctg ataaaaaaca ctggcttgga actgatgata gaggtagaga
    46021 tgtattcact cgaatttttt atggatatag aatctctctc agttttagtt tgatcttagt
    46081 tttcttggaa atcttaattg gaaccattat cggaggacta caaggatact tcgctgggat
    46141 atttgatata acgtttcagc gactcattga aattttctct gctatcccct ttttatatct
    46201 cattttaatc atgggttcct tcttcggaag aagttttacg attctattaa taacttatgg
    46261 cgctgtgagt tggattggaa ttagttacta tatgcgtgga gaattctata aactaagaca
    46321 ggctcaattt gtagaggcag caaaagcgtt tggagttcca tcctataaaa tcatttttaa
    46381 acacattgtt ccaaatgctc ttacgcctat tgttacattc ttacctttta tattaattgg
    46441 atcaatctcc attttatcgg cgcttgactt tttaggatat ggaattccgg ctccaaatcc
    46501 ttcttgggga gaattgattg gacaagggag agaaagatta agtgcttggt ggcttatagc
    46561 atttccctct tttgcactat ttttaacaat acaactcagt gcatttattg gggaaggttt
    46621 aagagatgca tttgatgcaa aggaaaagat agcattcaaa tgaaaaagaa aaatacaaag
    46681 atagtaaaag caaaagagac aaaaaaagaa tctcagcctt tgttacaaat tgagaattta
    46741 tctttgagta ttgcagctaa tacagatttg cctgtcttac aaagcatatc ctttcaaata
    46801 aaagaaagtg aagtattagc tttagttggg gaatctggaa gtggcaaatc actcactgca
    46861 ctttctatta caaaactatt gccgattaaa atttttaatt ataaaacagg atctattttc
    46921 tacaaagcaa aagatctact aaaagtagaa gaggaagaaa ttcgaaaaat tagaggaaaa
    46981 gaaatcgctt atatttttca agatccattc acgtccctaa atcctctaaa aaaaattaaa
    47041 gagcaaatta cagaatctta taaaatccat atttcggaaa atccaaaaga agccattgaa
    47101 aaagcaaagt atcttttaaa caaagtagga ttaaccgatt tagatgaaag attaaattcc
    47161 tacccgggag aaatgagcgg tggaatgctc cagagaattt gtattgcttc tgcactcatg
    47221 tgtgatccaa aacttctaat agcagatgaa cctactagtg cgttagacgt cacgattcaa
    47281 tcccagcttg ttgatttact tttaaaaata aagtctgaaa ataatatgtc cgttttattt
    47341 atatcgcatg acattgctct tgttgcctct atagcagata gaattgcagt tatgtatgca
    47401 gggcaagtaa tggaacaagg caatacagaa gatgttatca aaaaaccttt tcatccttat
    47461 agcgatgcac tactaaaagc aattcctgca ggaattaaac accacgatcg actttcgacg
    47521 atagacggaa tcgttccttc ccctgccgat tatccaattg gctgtcattt ctcgaatcga
    47581 tgtgcgaaag tttatgatcg atgtgaaaa
//
LOCUS       DAOQOS010000035        46490 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_304_length_46490_cov_6.736923, whole genome shotgun sequence.
ACCESSION   DAOQOS010000035 DAOQOS010000000
VERSION     DAOQOS010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 46490)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 46490)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46490
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_304_length_46490_cov_6.736923"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..4393
                     /locus_tag="PLF66_09645"
     CDS             <1..4393
                     /locus_tag="PLF66_09645"
                     /inference="COORDINATES: protein motif:HMM:NF019323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HMZ64345.1"
                     /translation="DPEKFLQEKVKERLVKVNVEKPAFADPIQALKDAFNFFKNDFRN
                     IARKELNTKTRSSISKGLKLDYKPIDSKNIAIPGILKEHKLVTSFSVPKSKQRWSYKR
                     VPVPLKDSGVYLVEAFANSHLAYSLVLKSNLHFVTKLSKIETLVYVADKRKGSPKEKA
                     NIKIWDNSSGELIADGKTDSNGIYLYKGKTQTKNLILVNHENQYAISDPSFYSSSFYS
                     EGGVKAYIYTERPVYRPGDDVFFKGIVRNFKKDSYFVTSGKASLDVFTSNGESILTGM
                     EVNLSQNGTFEGSFKAPEGENVFLGVYNLVLNYEGKTYATEFSVDAYRKPTFLVKVKP
                     EKQTYIKGEKIKFDISANYYHGMPLSGTEVEYQIFRKAKYDYSPVGSLNWEGTDAYLT
                     SKESSGRREFIKTEKGTLDKSGSLSSSFIPDDVSQDFIYTIIASVRDSDMTLTGASSF
                     SVNRSAIFIRIKKENQVFEPGAEVSFSAEAIAYDKSLAKEDLEKEIGNRKIKATIFKR
                     RFQGISQEAERKKIDSFSQKTNEEGKAQFKFSLADKGHFLVRLETEDRNGAETFTETT
                     IWSSAKSDSIEIPLKDLSLNASKDIYSIGDTAEILILSPVADANLLLTLEGNRILKYE
                     TISMKGNSLKYSIKITPDLSPNFTLSAVLFANLETYSGQIKVVAPPENKIVKVKIKSD
                     RETYKPGETVQLQISTTDLKQNGIKTELSVAVVDEAIYQIQEDKNPSLISYFYHPRRN
                     DVNTVYSSAYRFFGYSESRRMDLAFAKKKNLPLSVLKEDDSRSRERFKDTAFWLGTVT
                     TDSDGNAKVQFNLPDNLTSWRVTTIALTEDSKFGQNTYNFTTRKPISLQANLPPYLLR
                     GEKQSISTTVTNFTNQKEEVVVNLEARGVKIDGEQTKKITLKAHGSEVVSFSVQAEEV
                     NNTKSASLKFSIKGKFEDSVLKKVPLKFFGLKKIESVAMKIPSNGQKDKATLKLPNTA
                     TEEQLTLRFHPGNIEALRASLPYLIDYPYGCVEQTMSRFVPVLAAQKSVSLSLKQRDE
                     LPKMAAQGIKNLKTLLGSEGGFKWFSRDSLEDSMMTAYVYRALSIAKKLNTQVPEELL
                     NQSRNYLYKVLETTSDPFQKAYIIFSLSEGGEVEKSLIDSLISISGKQKLYGKALTGL
                     AAFASGQKDQAQSIYKKIISESGIYKGKIPKFEADIENDDIETISAILLLAVRLNDLE
                     ISESLSEELLNRKVDIAWKNSRDTGMAVLALAEKLEVFSEKLIPINLQIKVNGKDFKS
                     ISIKPTEILSGKSEWNFNVSSIAKGDNTIELIKEKGNTVYAIASLEYVDRSKNFNSFE
                     KGIKVKREYFSVLPEEKSGKTLLTAKNANSFKPGELVMVSIEVERSGKSDTYFMVEDP
                     LIPGFSFVTKDTNYYIGEYSADYESRQIYDDRAVFFIRGPAQKTIVRYFLRSDMSGNY
                     NVPPAKASLMYYPEIMGNSSSQNLKVE"
     gene            4397..5065
                     /locus_tag="PLF66_09650"
     CDS             4397..5065
                     /locus_tag="PLF66_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008590733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2135 domain-containing protein"
                     /protein_id="HMZ64346.1"
                     /translation="MNYLRFLFFLLFINGFQAITSSEVKIESPRGGFTTARVIEITGT
                     VADTSVERITLVMNGVPQTVKVQSGNFRIKSVVAPGSNSIEVKHGKHSDRVSFFASVP
                     SKDIKIVLTWDTPTDVDLWVIDPKGEKTYYAANSSSSGGNLDVDVTDGYGPETFTMAK
                     ALSGSYAVNVQYYSNNNIPITRVKLYVILYEGTSKEEKKYFEFTMTKGQQVYHIADFQ
                     IEGD"
     gene            5075..5998
                     /locus_tag="PLF66_09655"
     CDS             5075..5998
                     /locus_tag="PLF66_09655"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HMZ64347.1"
                     /translation="MHKFIIICFFSISFSLFGFDEVYLNQIIKEWEESKKNSAIVLIT
                     NPFSGRIEFVYNAKDAFYKKLPPGSILKPLSALTLLSQNDTKDKDTSFICKGKFWEPK
                     ENFFTRSDYKNFNILEDKESGKKYLKCSTEAGHGEINLREAISYSCNVFFLTKIFKSK
                     NLFYSSFVRDWNLFEGTGASFERISVSNFNDKIPNSPLESMLSGIGEDGKIKVTALKV
                     AQIFGAIFANTPFLKPIQKGETPEQVYPFPYSEKYRNIILQGLREAVKNGTLSDINLI
                     KNNVQILGGKTGSPTREGKKYSTHGWSIIFF"
     gene            complement(6101..6994)
                     /locus_tag="PLF66_09660"
     CDS             complement(6101..6994)
                     /locus_tag="PLF66_09660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015679458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HMZ64348.1"
                     /translation="MKERGSYYIVGVSFVLLSAIGFSAKAVMIKLAYYYRVDATTLLF
                     LRMAFSVPFFLVVGLHKSKSEKTNLQISDWIFVILLGFLGYYLSSILDFIGLVYISAG
                     MERLILFIYPTIVVLLSSFLFKQKIRSREIISLAITYIGILIVFFADKVEKTEHIYLG
                     AFLIFLCAFTYAIYLIGSGRMIPRIGAARFTAYAMLVACLSVTIHFFFTHKLESLQLP
                     SEVYVLSFLMAIISTVLPTFLLSQGIKRVGSGVASIIGTIGPVSTVFLAWIFLGETLN
                     LLQIIGTIFVILGVFIVSNRK"
     gene            7087..7587
                     /locus_tag="PLF66_09665"
     CDS             7087..7587
                     /locus_tag="PLF66_09665"
                     /inference="COORDINATES: protein motif:HMM:NF019235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1564 family protein"
                     /protein_id="HMZ64349.1"
                     /translation="MIQIKSKNSFKSPKSSFQENKTVSTLLIPNYLIEYLHCRVNENG
                     SLAKYLKLLLTNCRVFTHSGMLPPPDKIKTEYQKEGLDLTKVNFRVDNDDWIELGELA
                     LAFGKSRCYLFSYLVELDLLGFWDALSNFNMNLGAPTHPKLSLKVSWKLNRAKYSFKR
                     AYHVKI"
     gene            complement(7607..8842)
                     /locus_tag="PLF66_09670"
     CDS             complement(7607..8842)
                     /locus_tag="PLF66_09670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64350.1"
                     /translation="MLKSCRFLIEKIRVHFLPFCLLFFIHSSTNVFSTATCVGQACNV
                     IPQKYLSEFSNLDSTFQTQYLIPVMESMIESGIIINNNSSMIGSGYVNRFQLGSGLSA
                     SYIKKDDITINYGDITIPKLPNAGIASAPTIMGAINLGWFIGKGDALQQRNEDDDDEN
                     EDVDNKNYLHRFNLFFHGMNVGYNTTDVKAAYINKPEISGSIQVQSIGAILRYHIIEP
                     KISSYSLISFSGLSLGIGYNAQRLNMQINHQPRTNETVSFGELKGSWIAKTEFDFSSN
                     IHSIPIELRSGLKIFYFFDLFCGVGFSKNYGEANLHLSRGGPIKLSLDPTYIYNSSLL
                     TSDYYKYFSNNALNPTNGAMNPEGSLAMDFRRKVKLETNTNYFIAGMEFNFAILKLLA
                     EATIIEKGFGANLGLKVVF"
     gene            complement(8896..10872)
                     /locus_tag="PLF66_09675"
     CDS             complement(8896..10872)
                     /locus_tag="PLF66_09675"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HMZ64351.1"
                     /translation="MIKFLIKKLISICFIILVFCHSNAYAEDENDTKNPLVLYMDKIY
                     DVIPHAEFFEDKTGNLSFEEVLEKDFVRNDVNKYNFGYTSSVYWLRFQIKNQNSFLND
                     EWVFFIEYPQLDFVNFYFPIDHEEEEADVYIEKEGGDHFVFTKRDIESRVLNFTVPFE
                     IQKDPVIYVRIHSISAVQLPIYLAPQKLFYNRDNETQMLLGLYFGIMIIMLFYNLFLF
                     ISIKEKIYIYYVLTVFFSILYMISFHGFGFKYFWSEFPYMQNRIIHVSGVAMTLCLNF
                     FTVEFLDTKKFSPILNKILLWLRILSIPVIIISFILDSNLPIIISNSFSLIYPPFLIA
                     AGIMSLIYGNKAARLYLVGWGIASIVGTFNIFRLFGFVDDLRFLNYFSLFTGAFEVAF
                     FSFALADRINILKKAKTDAEDKLIQTLEESNQELEKKVKDRTKELSRSNKQNEALLQN
                     ILPTHVIERIKTHDEVLIVDNIPFASILFADVVGFTKLSQSVDATDLIVLLNNVFSHF
                     DVLTMVHNLEKMKTIGDAYMVVGGLPGSIDNHLEAMADLALDLLKFSHQQELIDLLMK
                     YPDFKIRIGMHCGPAVAGVIGVEKIAYDIWGDTVNTASRMESYGLPEMIHTSRQVYDK
                     LKGKYIFEKRGAIDIKGKGLMETYFLIDKFPPKK"
     gene            11436..12056
                     /locus_tag="PLF66_09680"
     CDS             11436..12056
                     /locus_tag="PLF66_09680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64352.1"
                     /translation="MSTSYKKNSQTIDLTCSSKGNSSYLLKVTVSHNEKGIIYKVTSV
                     LFAHGWDIEEAVAETVDGRLVKDVFLIRNLEGKKLSEQAFQKIRSDLNSLFYEGNSVT
                     EYLQASDKNVNAFKKPYNSTVNVFNPPSIEFTVLDIRTLDRPGLLYEISQILYLYDID
                     IISFTAKSENHVIRDSFLICTPNSQKLSEQECNRLKDKLLSILSPK"
     gene            complement(12058..13149)
                     /locus_tag="PLF66_09685"
     CDS             complement(12058..13149)
                     /locus_tag="PLF66_09685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020771427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64353.1"
                     /translation="MDEESRFSNYKNKVKDFFNLKKFNQIPNFIELLEKGLEKELSFH
                     HTKTDPPIPVEELPIDLSLRKSGIVRKVYEKIFPVTHVFQVTYKFNGVFMENFMPISN
                     DDFIGRGGYKFVYRLPWNQVVKVGKSKLGSDPLFGSLFKEVSKNLEMYLKPEELELKN
                     YLEKQCFKNSSKELLDFKFKRLGLERLHYWKVKTLLPDLVLPTKFFMGLRSRRTPIGI
                     PQTTITPCDNQILIPGKHLKEFVKLTEKVKQSTLEDRFLPKWKLNFDTHKFGKVPVSK
                     LKKIAFNFHRVIEATRYLAQKEKLILDLHSENIIIMLPEFELRIFDFHVFDEHLYDPG
                     EDGLSPEEEHINTINEFINSFELGKHPEE"
     gene            complement(13142..14992)
                     /locus_tag="PLF66_09690"
     CDS             complement(13142..14992)
                     /locus_tag="PLF66_09690"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004450854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HMZ64354.1"
                     /translation="MKQKILQSIQKQLEEVFKLKQFREGQKEAIEALLAGRDVISIFP
                     TGGGKSLLYQLPASIQKEGVSIVISPLIALMKDQVESLNKIGVSASSCNSSQDELEQL
                     TAISRAVTGKIKLLYISPERALSSYFTTMLKKMKVNFFAVDEAHCVSQWGHDFRPEYR
                     ELYRLRQLHPNSPFIALTATATEKVLNDIENSLNLKNSYIVKKSFQRPNLSFQIEYLR
                     NETEKEIKLLEILNSEFKTKNHGRVIIYCATRNKVDEVCKFLLENGFKATRYHAGKTD
                     SMREKAQDAYSSGKVKILVATNAFGMGLDSPDVRLVLHYQIPSSIESYYQEAGRAGRD
                     GKNSRCILFFQNADLGIQNFILRKESNHKGGDTLLDAIKAYAFSNVCRQKFLCSYFGE
                     EIQTCGICDICKANIPNPHNDYLEELQLKLEKKVLRGKYEFASDELNIIRSLLISAPA
                     VYGKKMIAGILRGSKAKNILRSKADQSSFYGKLSTIPEEAILTKLEEWIEKRELKIAG
                     KKYPKIYLSSHPPMTRKEKIESEIQSGLRTRKIQTPNQVLVKALKNYRDREARKYRWK
                     KFMVLHNSVITRIANLKPQTKQDLIHIKGLGDAKIEKFGDGILDTVRKYG"
     gene            complement(15338..15589)
                     /locus_tag="PLF66_09695"
     CDS             complement(15338..15589)
                     /locus_tag="PLF66_09695"
                     /inference="COORDINATES: protein motif:HMM:NF016141.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HMZ64355.1"
                     /translation="MLFKLIGMVLVGALVGYIAGRIYSGSGFGFVKNMIIGIIGSLIG
                     GLLLSLVGFASTGIISSLISDLLGALTLLWIINRIAGKK"
     gene            complement(15600..18446)
                     /locus_tag="PLF66_09700"
     CDS             complement(15600..18446)
                     /locus_tag="PLF66_09700"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HMZ64356.1"
                     /translation="MKILFTIILLSCFQLLADPIGKDPILKVEAGMHTAVIRRISTDA
                     ENRLLATASTDKTAKIWELSTGRLLRTIRVPIASGHEGKVNAVALSPDGRTLAVAGWT
                     GLEENDGKFIYIFDVETGNLKRSISKVDEIIYHMAYSPDGKYLAVNLKLGFKIYNGIN
                     FNLVAEDMDYRDRSYDTGFILQNGSLRIITISLDGFIRYYTLESNKLKLLDRKSGQNA
                     RIPISMRVSSDGKKLAVGYDLTTTVEVFDIEKDTINYSFAPSVSGIGNGDLSKVTWSN
                     DGEYLYAGGMAQKNGYFIRKWSNGGRGDYKDLPASWNTIMHVIPLKDGGIAYSTHDPS
                     YGILDSQDRRLIYKLQDTPDYRDAQEKFLVSEDGKRVQFGFLQFGKDQAQFSLTDRRV
                     YLDELNTDGMYKPDTSSLRITNWRNNYSPLLKGNRIVLEDHETSRCLAIQDDKFILGT
                     EWNLRYYDKSGTELWKVPAPGIAWNVNLTSNGKYAIASFGDGTIRWYKTEDGTELLSL
                     FLHKDKKRWVISTASGYYDTAPGTEEFLGWHVNRGKDHSADFFPIAKFRSKYYRPDII
                     DRILDIGSEQETIQQTNNESGRKKDTGTITDHFPPVIEIISPKSIYKTGNKSVFIQYT
                     VRTPSGNPVSGIRVLIDGRPITGTKGMNLVAVEKESPARSIRIDIPPRDCVISLIAQS
                     KFNSSEPANLQIDWENKLQQDQEKPRLFILAVGVSDYQDSSLQLQFGAKDASDFINTM
                     QRQKGKMYKDIFVKSLLNKEATTSNILDGLEWIQKETKANDYAMIFMSGHGVNDSIGN
                     YYFLPHNFDPEKFKSTGVSYLEIKNTLTSIQGKVVFFGDTCHSANVFGKGNDITILIN
                     ELSDTENGIVVFTSSTKNQLSLEDKSWGNGAFTKALVEGLNGKADYSRKGKITINMLD
                     LYISERVKELTKEQQTPATSKPDTIADFQIAEIK"
     gene            complement(18461..19780)
                     /locus_tag="PLF66_09705"
     CDS             complement(18461..19780)
                     /locus_tag="PLF66_09705"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HMZ64357.1"
                     /translation="MRFKLKLFFLLCFMTFTVYADPNIELLSSASIGDLDGVKKSLAQ
                     NADINFRDSLGTSALMFAANNNHFPVVKFLVDNKADVFAKNTNGWTAAVMAGARGNTF
                     IKEFLEGTERKIVASRSSGVILALVGDVTVGNKKINVGDIVEENQTIFVGKKSYCDLQ
                     VKQSLSNFTIRVRENSVFSLKFKEKPDENVYSGFLKFGSALFKVGKVFSGERIEVISP
                     TAIAAVRGTIFDTSVANDGSTKLNVQEGFVQTRIRAPEAEDDTTIEDTIEMKRLTENL
                     ERNAQLVPYGYSVETSPKNQRDVLQKMDAENLVKRAKRKEYSTTSIQEVNLKCSEKPK
                     LKKGIEESSVKRSREESESLIGIDGSKLRNTWDRSDLLNLIQNRKVSQDSSNARAQVI
                     RQPEKIKLKNGSVITGSIYQLDNQYFLISEQGEQKINEADIDVVYFN"
     gene            20074..21339
                     /locus_tag="PLF66_09710"
     CDS             20074..21339
                     /locus_tag="PLF66_09710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64358.1"
                     /translation="MKKSLFALVLLAPFFSACQTISKFFASAPPPPQPQPAYVAPRIY
                     PEKINLSEVKVEAINEAPNEPLGVYKGSDGNNYLFLNLIYENSGLSVIEAIKNGEILD
                     NMIIVRDQTGKIIGSDVVNFQAFATIEKKLFKNTQEIVRTKDGFETPVRPQSAKRDLE
                     PEISTSYQSKIYTEKEKVPQVIKLNELPANNSYISINLELVYVEPFIQQICDKNGKNP
                     CTYVDEVVTKEVREANKKKLEAIIAEYQKTPTDKQPAEKRSPKKFILTPAEIQAFKDK
                     MKKNVSKTGITLEGEKTKVADSVVDVEKAKLNYREWSLVSAPKYFQVNTEVIKPLVGE
                     APSAIPGNITPAPVNPMNPVTPNNNTNSITNPSADLPIEGFSDTVTLKNGLIFKNVKA
                     TIKESTVLITSQDGKTMEINKSELVSIKK"
     gene            complement(21395..22969)
                     /locus_tag="PLF66_09715"
     CDS             complement(21395..22969)
                     /locus_tag="PLF66_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016545687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HMZ64359.1"
                     /translation="MEKENHFDAIVIGSGMGALTTASILARLKNKKVLVLEKHFKLGG
                     FTHTFKREGKFLWDVGIHYVGDMGKQSMLRKLFDIVTEGKVDWNPMPDLFERFVYPEI
                     TFDQYSSEEKFRSDLIQKFPHEKQAIENYFSDVKSFSNWATKQFTLKAKPMLLDTESN
                     NFKLTGAKSPMITTKEYLDANFKDPVLKAVLVSQWGDYGLPPSTSAFYMHAGLVVHYL
                     NGGFYPVGGAGTIAESVKPIIEKKGGAMHIFSEVTEIIMDGEKAIGVKVKQTKGKNIL
                     EKEYFAPIIISDAGVYNTYTKLIPQNVEIPFRESISNFVTGASSVTLYLGFKENPAKL
                     GFKGENYWIYNSIDHDKNFNEKDNLLKGEPTAFYASFPSLKDPKAESFTGEVISFVNY
                     EPFAKWKNGEWKNRGTDYKELKDRIADGMLNFMERHFPGYKDLVEYKELSTPLTNEYF
                     TDHKAGTIYGIPCTLERYTKEWMGVKTPIENVFITGADACSPGISGALMGGFAAATVV
                     LGYNSMMGIMKDSAKI"
     gene            23068..23679
                     /locus_tag="PLF66_09720"
     CDS             23068..23679
                     /locus_tag="PLF66_09720"
                     /inference="COORDINATES: protein motif:HMM:NF024701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HMZ64360.1"
                     /translation="MKKGKSSSNYHHGDLKKALLDSGVSILAEQGIKNLSLREAARRA
                     GVSHTAPYRHFEDKDALLAAIAEEGFNRLILKLEEGIAKHPNNPLQRHYAIGEAYVQF
                     ALENPNHLQVMFGNVLEDYKKYSSLKQVSDKSFSLLVETIKDCQKAGIVKKSNAIQLA
                     LVAWSAIHGLSLLTIEKRNKWISENNSRIIKILGDVLFNGMKA"
     gene            complement(23693..30562)
                     /locus_tag="PLF66_09725"
     CDS             complement(23693..30562)
                     /locus_tag="PLF66_09725"
                     /inference="COORDINATES: protein motif:HMM:NF015140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007154 - cell communication [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Calx-beta domain-containing protein"
                     /protein_id="HMZ64361.1"
                     /translation="MIFTSNSKFLILNILISTLFFTNCTAWPAISAILLSGNKKSGMI
                     PLLPIGGSSPANGSQTTTTPETPPTAGNTSTTTPSIQFGSSSSTNLETSPSVTIPVTI
                     SVGSTATVDYTVSGTATGGGVDYTLANGSLTFSSATTTQNISFPIVNDALQEANETII
                     ITLSNPSSGAVLGSITTHTYTIVDDDTPSIAFNATTSNGSEATTSVSIPVSISMTATA
                     SVNYSVTGGTATGSGTDFTLASGVLNFTNGGATSQNINFTVNNDTLYENNETIIITLA
                     STTNANLGIDTVHTYTINDNDPVPTLGFSVTSSSGAESATSPTLNVTLSTASGLTTNV
                     DYSVTGGTATGGGVDFTLNTGTLVFNPGTTTLSLPLSIINDTNTELNETLIVTLSNPT
                     NANLNANTVHTYTVLDNDPPTVAFNTNSSSGSESTTAVSLPVSLSAVYASTVTVNYAV
                     TGGTATGSGTDFTLASGTLTFTSGTLTQNINMTVVNDLLGESDETVIVTLSAPSNATL
                     GTFTSHTYTINDNDPTTVEFSSSSSNGSEGTTAVTIPVTLSNSNASTVTVDYSVTGGT
                     ATGGGTDFTLASGTLTYTSGVTSQNISITINNDILLESNETIKISLANPTIATLGTNT
                     EHTYTIIDNDTPSVGFSASTSSGAESVTTVNIPVALSIVSGSTVTVDYAVTGGTATGS
                     GTDYTLASGTLTFNSGVTSQNISIAVNNDTLNELNETIIISISNPTVANLGAITSHTY
                     TITDNDPIPSLSINNVSVTEGNAGTVNADFTVTLSAASGRSVTVDYSTSNGTASAGSD
                     YTAIPTTTLTFNPGETTKTISVAVNGDSTDEVNETFNVSLANVSTSATIAVATGVGTI
                     TNDDTSPTLSIDNVSVTEGNASTVNATFTVSLSVASGKTITVDYVTANNTASSGSDYT
                     AITTTTLTFNPGDTTKTFNVVVAGDTLDETDETFYVNLSNVNANATIATSQGIGTIVN
                     DDMPPTVQFSASTSNSVNETQTNRTITLSLSAASGKTVSVVVTDLGTGTATAGSDYTN
                     IGSPLTVNFTPGSTTANVTIAVLSDLLFEGNDTIDLSISSPTNASLGTQTTHTFTIID
                     DEIGVTSAETMDCDNNGKIDHYKLTFNTLVTDSTFPGYVLNSVGSVTTHWLVAGYSNV
                     KLQHGTSVASVCSGFSDTANDAVLYLAFTEGVGFDTGAKPDLTTSSTPTLTGPTGTAA
                     QLYTATVTESDRAKPVVVSASGNAGETGLTVTFSEAVYGTNGAPSCGSGGDLSATTLT
                     YANSNAGGATSLSSMGADTCATSDSNAIFTANANFIAGDGDTSAPDDSVAGNSNLYDA
                     ANNTGNTTAKAISISAGNPTISLIEEYDTNGNGKIDQIKVVFSLSMNDATIDDLDASR
                     FTMGGTAAIKVDTATSGTGTIVTPNTDTGVADDNTITLFTDDNTVSGTAVKAMAFTTS
                     AGRWRSITGVELQTISNLTAVTVDKAPPVILTAVASPTGNTNATVDSGDTLVITFSEP
                     TDKSITISNIASVLSLSSSHVWGDLTSANWNGAGDILTLTFAGTGSPTVAVGDYITIV
                     NTLKDTASSPNTSTNKVSVTPISGSFGVDSTSPYLVYTSDITTNSLIVQFSEAMRMGS
                     GTANQNADSLANYTLIEDPTNAGCADATLASLSVLSPTSVKINLSGGQSFCNIQYRLT
                     VASTVTDLAGNAMGSPKFLTFIGLEKIKVVAATALTPNTIKITFSKPPLTGMNTTNSA
                     ECNSAYECGLRYKISPTIGDGTISSAVVGSGASNFTVTLTHNGSQAGVAYTVIVANAT
                     DNDNFNNGSVCIRDSAGTNCIQSAPNDRATFIGAGTACTTLACGSFFEDPFFDGTTFS
                     FAFKYDGKVYLGTNDKNNAAFRFDPNGTNAILTSFAFVNNSSVSLSCAAATGFGYITT
                     DLSTQTCGTNGGANGEVGAVGFNSINLTISSTNYELLGIGALKNNVDTVYYSQDKDIL
                     LDMKSFTITGTNGINSKSAQTLYGNADKVFNGISSDHGTNAPVLNRLPATAASGVVTI
                     GTPVDLSGKNITVLGKAPAAGEPTNPNSSGVVGIDVIFTQGASTYLANNGGVIYVTTS
                     KILSATPGNANDWKLGTTTFDPVLSTPSAATGTSLYMPASNSTTGGGLGKVRPGEKAY
                     PYIILYNGKLYMVRNVGDTTPTTNLRGEVWTCTPNASGQCAPAGWSKIISGTETDIGS
                     TATAIGMLIANGNNLYLGFDDPVSGVRVFKFSGTTPSATSGTMSGAGWVQQGTNGLGS
                     NSKYIMTSVSLYDQITNKNYIYIAIGNGLGTTAIKVVRQVD"
     gene            complement(30649..31113)
                     /locus_tag="PLF66_09730"
     CDS             complement(30649..31113)
                     /locus_tag="PLF66_09730"
                     /inference="COORDINATES: protein motif:HMM:NF013996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin family protein"
                     /protein_id="HMZ64362.1"
                     /translation="MLFIRFLKFFFTFIILFNLSFCSNEMEQKSNEPIRPKEKVSPKT
                     PKSEYTEIDSGEWEDIKNNHLPQIKISPKSKKENVEISVTGEFNEDHYIERIGVMDEN
                     KQDIAGIDLERKQIPHIKLTLDPFPENPRVKVYVKCNLHDLWTKPLLPTKKN"
     gene            31427..31594
                     /locus_tag="PLF66_09735"
     CDS             31427..31594
                     /locus_tag="PLF66_09735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64363.1"
                     /translation="METLTKISSTEEENETSTQNKDKKDTKPKFSTKRDPSVRMSYIV
                     PEEISSEDLDS"
     gene            complement(31721..32224)
                     /locus_tag="PLF66_09740"
     CDS             complement(31721..32224)
                     /locus_tag="PLF66_09740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64364.1"
                     /translation="MKVFLILIVFYSSLFAKDSFTSEYTNISKDCKYDSKDIQEGQDA
                     PLICNGPSGYTVIISYSACMENLRIESKNKKESIDMPSQPIGSAETRKLEWRLLNKKP
                     IGVIYRTFVLKDSPNGNCPQEKTKQETLEVRGLGKYLELNTSIASGKNANEKARDALE
                     SFVIAKK"
     gene            complement(32240..33277)
                     /gene="purM"
                     /locus_tag="PLF66_09745"
     CDS             complement(32240..33277)
                     /gene="purM"
                     /locus_tag="PLF66_09745"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004479398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HMZ64365.1"
                     /translation="MSNNPITYKDAGVDTEAGRDFVNSIKKNVHSTHNPRVLGGLGGF
                     AAAYDVSFLKNYKEPILLSCTDGVGTKLELARLLDKHDTIGIDLVAMSVNDLLVSGAR
                     PLFFLDYISCGKLDIQKMEKIVSGIVTGCKLCGAALVGGETAEHPGTMKPDEYDLAGF
                     AVGAVEKSELIDGSKIQSGDLIVGIESSGPHSNGYSLIRKLYLENGALPSDKQALAFI
                     SDYLMKPTRIYVDPILKLLQKEEIKGMIHITGGGFYENIPRIFGKELAARIIKKDLPE
                     SYVFNKIKKDHSLDEKDMYSTFNMGVGFILIIPKDRGNSVLQELAALGESAKIIGEII
                     NRNSEEVEMVD"
     gene            complement(33267..34046)
                     /gene="murI"
                     /locus_tag="PLF66_09750"
     CDS             complement(33267..34046)
                     /gene="murI"
                     /locus_tag="PLF66_09750"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HMZ64366.1"
                     /translation="MSDTRVKIGVTDSGFGGLSVLSELSNVIPEADFIYYGDLLNSPY
                     GNKSPDQVYTFIDEICRFFISKEVSAIVVACNTATSVAIHKLRKKYHIPIFGMEPAIK
                     PAVLENPDQKIAVLATPLTLKEEKFMRLGEALEAHTILKPIPCDGLATIIDSGDMDGA
                     RKYLQPIFKDLEEDKINNIVLGCTHYVLIKSLFYEWNPNIKLYDGNRGTANHVRRIIT
                     PLPNEGESKLDLFLNGGTEADFELAYNYLNNETTTERNYVK"
     gene            complement(34039..34389)
                     /locus_tag="PLF66_09755"
     CDS             complement(34039..34389)
                     /locus_tag="PLF66_09755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64367.1"
                     /translation="MISRKYFIYAFFVMITYSALLAECKLCPDLISIKVILKNGVIRT
                     GHMSYGPEEELREKFDRSMEVIRKKEKLRLFLSLDSIQNLNSLSIGANTIYIDIPYKD
                     IAKIERLEKIDSNE"
     gene            complement(34406..35407)
                     /locus_tag="PLF66_09760"
     CDS             complement(34406..35407)
                     /locus_tag="PLF66_09760"
                     /inference="COORDINATES: protein motif:HMM:NF018805.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin-like domain-containing protein"
                     /protein_id="HMZ64368.1"
                     /translation="MYLSLDAFEFPKDHSFHKDFQIEWCYFVGNLKAKTGQDFGYELS
                     FFRANMRGKKEIFPVHFAISNLSTKRHYTSQSLERRIGNLAAYTSYVIKNGDYKIEIL
                     GEAKFRISAKPRKDLISLDLLLTSTRNDILLHGKEGYSQKSNLDSNIYSYYYSIPRLI
                     TEGYIQIESQRFEIESGTSWMDHEWSAPEKDSSELKSTIVSEENSWDWLCLNLSDGSD
                     LMLFNFRKNKNTEPETFGTFRDSNGRIETFKPDGKMKLESLNEIWKSPITNKNYPMKW
                     KVIGEDLNLVVETRFNNQEFIGFKTTGNTYWEGAIVATGIYKKQKINGKGYLELKGY"
     gene            35746..36810
                     /locus_tag="PLF66_09765"
     CDS             35746..36810
                     /locus_tag="PLF66_09765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde reductase"
                     /protein_id="HMZ64369.1"
                     /translation="MLISNSSKPILVTGGTGYIASWVVKYLLEDGHSVHTTVRDKSKK
                     DKYAHLEKIAQESKGSLSIFEADLMKKDSFFEAMQGCELVIHMASPFFVVGIKDAMKD
                     LIEPAKDGTANVLNSVNQTASVKRVVLTSSCAAIYGDNCDIEQTKNKIFTEEYWNRTS
                     SINHSPYSYSKTVAEEEAWKIAKTQSRWDLLTVNPAFVLGPSLTKRTDSTSINMMIQL
                     GNGMFALGVPELWFGIVDVRDVAKAHILAAFTPSAKGRHIACSECLPMVDIASALREK
                     FGDHYPFPKWNVPYFMVWLFGPLLGFSHSYVEKNIGIPIKCDNSYTQKDLGIQFRSAK
                     ETFWDHFEQLLNDGIIQRKG"
     gene            complement(36811..37812)
                     /gene="rfbB"
                     /locus_tag="PLF66_09770"
     CDS             complement(36811..37812)
                     /gene="rfbB"
                     /locus_tag="PLF66_09770"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020777512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HMZ64370.1"
                     /translation="MNRSLKKLLVTGGAGFIGANFIHYLAKIGFKGHVLNLDSLTYAG
                     DLGNLTGIEKNLNYEFMKGDIRDKDYLNFVFTKNEIDTVVHFAAESHVDNSIAGPRIF
                     AETNVMGTFELLTAARTYWQKDGKYKEGVHFHHISTDEVYGSLGETGFFTETTPYDPS
                     SPYSASKAASDHFVNAFHRTFGMPITISNCSNNYGPYQNKEKLIPLMITNAINGRKLP
                     VYGDGKNVRDWLYVEDHCDAIWTILQKGESGRSYNVGGNNEWTNINIVKKICEVLGKL
                     TNKPVSEYEKLINYVTDRPGHDRRYAIDATRIQKELNWFPKETFETGIEKTVKFYLG"
     gene            complement(37859..38488)
                     /locus_tag="PLF66_09775"
     CDS             complement(37859..38488)
                     /locus_tag="PLF66_09775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64371.1"
                     /translation="MIQLKTDAIKVFESLSIPEAKALSIFGRITDCYSESHRSYHNLR
                     HIREMLETIFLYKENIVDYSLLYIACLYHDIFYDTHANDNEEKSYEYLKKDFSEYLSS
                     EQIEKCGKLILGTKTHQFLQDDFDNKIFLDSDLLILGRERNRYIEYMDSIRKEYDWVE
                     REIYKTERSKILKKFLERNRIYFTDEIYSKYEKEARENLNFEIEMYFQK"
     gene            complement(38495..41608)
                     /locus_tag="PLF66_09780"
     CDS             complement(38495..41608)
                     /locus_tag="PLF66_09780"
                     /inference="COORDINATES: protein motif:HMM:TIGR00914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HMZ64372.1"
                     /translation="MGFNRLTEFVVHRRFLTLAIIFIFCLVGIYSWTQLEIVAYPDIS
                     DIEVSVITQVNGLPAEEVELQVTVPIERALNTVPGVISKRSKTIFGLSIVRLTFQDNT
                     NIYLARQLVEEKLRDADLPPIASPVLGPMTPSIGEIYRYVIEAPDNVPLTQLRELQDY
                     VIIPKLLQAEGVVDIANFGGLVKQYQVIINPIQLEKYGLSIQSIADAIHSNNENTGGS
                     YMILGSSQMNIRGIGRITKLEDIGNIVVDNRHGVPILIKDIASVEIGVYPPTGVLGYL
                     DKMRKKEDDAGIEGIVLLRKFENPTATMQNVLAKVNELNNGLLPDGVRIETLYDRTEL
                     VALTIRTVAQTLIEGTVVVFIVLTILLGSWKAAVISSLAIPFSLLFAFVCMDIANIPA
                     NLLSLGAIDFGIIVDAAIVMVEGIFRHLSEKKEEDNTNLSHLVIRSSAEVQTQILFAV
                     GIIILALLPILTLQRVEGRMFAPMAWTLSFAILGSMVYALTVVPVLSTFLFQNSKIAH
                     ENPIWIRIQNGYGEILKKAFSIPKPVLGASFGFVFFIFSISPGLGTEFLPELDEGSVW
                     VKIFLPSGISLEAARQYPDVVRKELGKFDEVRAVLTQLGRNDDGTDPYGQNRLEVLVQ
                     LKQPYSKWESKITKKELITEFKKTLELLLPGAAFTITQPIIDTTTENATGSSADLAIF
                     ITGRDLDVLRDIAVNILELTRSTDGASESSIEQENKQSQVVIRIDKQAAARYGINVED
                     VNGILKTAIAGLPVSHLYEDERKFDIVLRFTVESRNTPQSLEKILIPSRTGQRIPLNR
                     VASVGLEEGQTIICREDGRRQMTVKTNIRERDQGSFADEIRNKIEKNIPFPENVKFHL
                     GGQFENLKRSQERLTYIVPLTLLLIYLTLLVFFKNNYIHAAIVTANIPFAVIGGVIGL
                     YIRGMHFSISAGVGFVSLFGISVMSGVLLLSFLNHMKHNTDLPLKEMVIQGSVIQFRP
                     RFLVMMIAIIGLMPAALNTGIGSDVQRPLATVIVGGLFSSLVLTMLVSPLIYYILEKR
                     FQKNSRR"
     gene            complement(41598..42587)
                     /locus_tag="PLF66_09785"
     CDS             complement(41598..42587)
                     /locus_tag="PLF66_09785"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HMZ64373.1"
                     /translation="MRFSTKIFILFLILLFSLESCRCKKDRNEEKKPLPLSGSGAMIH
                     EGGKHIEFPPDAPQINLFQTQKVVFLSYDLNINAPATVIGRIKKAEAPGLPSIILFAS
                     PDLTSAYAAYLQNLSLIHIAQTNYNRFKDLADHGAATGKELNDAAAELYNRQSSLAEN
                     EAKLRREGFNPKDLQRASRGSVWLISDVPESELNILIEGLTCSLSFPSFPTETFSGKI
                     DAIAEVLNSDTRKARIRIIMHDTKDKIRPGMYGKVQFKMPESGLMIPKKSLVSSNARY
                     YVFIKISPTTFQRREVTISTDTEEFIEIAHGITAGEEIVTTNVFLLKGLSFGI"
     gene            complement(42598..43875)
                     /locus_tag="PLF66_09790"
     CDS             complement(42598..43875)
                     /locus_tag="PLF66_09790"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HMZ64374.1"
                     /translation="MTFILTLFCFLSTVSIYSIDLSKPLSLTDSLELARKNNPFYIAE
                     QQNIAIANADVTTSKLLPNPLLNNQELIRQSPASGALPYLPASGGRGGAWNDANRQDW
                     IQLTQKIPVAGQRKYSIELALKNLQFSQKNLVEFERNLLYIVANKWIDVWFANQRLNI
                     IKRTKEYSDDLLKINEIRLRNQVITQSEYLRTQNIVEQYEANLASAEMALKKESRNLQ
                     FLSGVNTEILINVEDNKISYEKLDESKESLIQYALGNRADIVANKVHQEVHRVNINLQ
                     EAYVYPQPEVGVIYNPQNGSPYLGTYVTVPLPVFNRNQGEIQKSHSQLKQVMNLQEAL
                     EKQLDTDVQNAIGEYNLSKANYERYKKILETTEKVLNTVRYSYLKGGTTVIDYKDAQR
                     SWFDAQNSFYESIFNLRKSYIQVQYVTGKIQNF"
     gene            complement(43930..44607)
                     /locus_tag="PLF66_09795"
     CDS             complement(43930..44607)
                     /locus_tag="PLF66_09795"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HMZ64375.1"
                     /translation="MDIKEELKSTFYLDADSTEVKEFIQNLNLEELNDVEKAKKIYLA
                     VRDEIRYDPYNIILTPEALRASAVIKRKYGFCVEKAVTLAACLRSAGIPAKVGFANVK
                     NHLNSKRLQDLMQSDIFVFHGYTDIFLNKQWVKATPAFNLSLCERANILPLEFDGEHD
                     SIFHPLDKSGNKHMEYLVDHGSFIDLPFDRMMDEYDHFYRHLRVKETKQFGNINKAKF
                     EEDVNRS"
     gene            complement(44634..45245)
                     /locus_tag="PLF66_09800"
     CDS             complement(44634..45245)
                     /locus_tag="PLF66_09800"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HMZ64376.1"
                     /translation="MIDLESLRDVTSIETMIELLRRRIGSPVSYASLARDLQKSPATI
                     KHWFEILENLYIVFLVTPYNKNISRSILKEPKFYFYNIAITEGEGARLENLVACSLHK
                     ELHRLEDFERIKTGLHYLRTKEGEEIDFLITLDDKPSLMIEVKLGEENPSSHFNTFNN
                     YFPQIPKFQLVMNLDREKGYDWGLKIRRVSKGLTHFNLISFLL"
     gene            complement(45260..45433)
                     /locus_tag="PLF66_09805"
     CDS             complement(45260..45433)
                     /locus_tag="PLF66_09805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64377.1"
                     /translation="MGRFFQYRLHPFDLKELANQGSSDEILKTLLAIGSFPDHLQKAR
                     NPFIKNGIVPIWM"
     gene            46025..>46490
                     /locus_tag="PLF66_09810"
     CDS             46025..>46490
                     /locus_tag="PLF66_09810"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001141234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HMZ64378.1"
                     /translation="MPSSWGKIFRVSTFGESHGDSVGVVIDGVPGGIPFKLEEIQKDL
                     TRRRPGQNLLTTPRKEDDEVRVVSGIFEGKTIGSPITLIVDNKNHLSKDYENLRHIYR
                     PSHADYTYHAKYGFRSHVGGARASVRETIGRVAAGAFARCILEEELGIQTIAW"
BASE COUNT        15285 a         8656 c         7278 g        15271 t
ORIGIN      
        1 ggatccagaa aagtttctac aagagaaagt aaaagaaaga cttgtaaaag taaatgtgga
       61 aaaacctgca tttgcagatc caatccaagc attaaaagat gcatttaact ttttcaaaaa
      121 cgattttcga aacattgcaa gaaaagaatt aaataccaaa acaagatctt ccatttcaaa
      181 aggcttaaag ttagattata aacccattga ttctaaaaat attgccatac ctggtatttt
      241 aaaagaacat aaattagtaa cttctttttc tgttcccaaa tcaaaacagc gttggtcata
      301 taaacgagtt ccagttcctt taaaagactc tggtgtttat ttagtagaag cttttgcaaa
      361 ttctcatctt gcctattctc ttgttttaaa atcaaatctt catttcgtaa ccaagctttc
      421 caaaatagaa acgttagtct atgtagctga taaacgaaaa ggatcaccaa aagagaaagc
      481 aaacataaaa atatgggata atagttctgg tgaactgatt gcagatggaa agacagactc
      541 gaatgggatc tatctttaca aaggtaaaac gcaaacaaaa aatctaatcc tagtgaatca
      601 tgaaaaccaa tatgcgattt ccgatccgag tttctattct agctcctttt atagtgaggg
      661 aggagttaaa gcttatatat atacagaaag gccagtctat cgtcctggag atgatgtctt
      721 tttcaaagga attgtgagaa atttcaaaaa agattcttac tttgtgacta gtggtaaagc
      781 ttctctagat gtgtttacta gcaatggtga atcaatatta acaggcatgg aagtaaatct
      841 ttcccaaaat ggaacctttg aaggaagctt caaagctcca gaaggagaaa atgtttttct
      901 tggagtttat aatttagttt tgaactatga aggcaaaaca tatgcaacag agttctcagt
      961 ggacgcctat cgcaaaccaa ctttcctcgt gaaagtaaaa ccagaaaagc aaacctacat
     1021 aaaaggagaa aaaataaaat ttgatatcag tgcaaattat tatcatggga tgcctctttc
     1081 tggaactgaa gttgaatacc aaatatttcg aaaagcaaaa tatgattact ctccagttgg
     1141 atctttaaat tgggaaggaa cagatgcata cttgacatct aaagaatctt caggaagaag
     1201 agaatttatt aaaacagaaa aaggaactct cgataaaagc ggatctcttt ctagttcatt
     1261 tataccggat gacgtttcac aagattttat atatacgatt attgcatctg taagagattc
     1321 ggacatgact ctaactgggg ctagttcttt ttcagtaaat agaagtgcca tttttataag
     1381 aataaaaaaa gagaatcaag tttttgaacc gggagcggaa gtaagttttt cagcagaagc
     1441 aattgcttac gataagagtt tagcaaaaga ggatctagaa aaggaaattg gaaatcgaaa
     1501 aataaaggca actatattca aaagaagatt tcaaggaatc tcacaagagg ctgaaagaaa
     1561 aaaaatagat tctttttctc aaaagacaaa cgaagaagga aaagcccagt ttaaattttc
     1621 attagcggat aagggacatt ttctggtgag actagaaacc gaagatagaa atggagcaga
     1681 aacattcact gaaactacta tctggtcttc tgcaaaatca gattcaatcg aaatcccgct
     1741 caaggattta tcactgaatg caagtaaaga tatttactcc ataggggata ctgctgaaat
     1801 tctaattcta tctcccgtgg ctgatgcaaa tttattacta acattagaag gaaatcgaat
     1861 tttaaaatat gagactattt cgatgaaggg aaattcattg aaatatagca taaaaataac
     1921 tccagattta tcgcctaact ttacactatc agctgtttta tttgccaatc tagaaacgta
     1981 ttcaggtcaa atcaaagtag ttgctccccc tgaaaataaa atcgttaaag taaaaattaa
     2041 atcggatcga gagacctata aaccaggaga aacagttcaa ttgcagattt ccactactga
     2101 tctaaaacaa aatggaataa aaacagagct gtcggtcgca gtagtagatg aagcaatcta
     2161 ccagattcaa gaagataaaa atccatcttt gatttcatac ttctatcatc caagaagaaa
     2221 cgatgtgaat actgtttact cttccgctta tcgtttcttt ggatattctg aatcgaggag
     2281 aatggattta gcttttgcaa agaaaaaaaa cttacctctc tcggttttaa aagaagacga
     2341 ttctagatct agagaacgtt tcaaagatac tgccttctgg cttggaactg tcactacaga
     2401 ttctgacgga aatgcaaagg tccaatttaa tctaccagat aacctcactt cctggagagt
     2461 gacaactatc gctcttactg aagattcaaa attcggtcaa aatacttata attttacaac
     2521 aagaaaacca atatcattac aagccaatct tcctccttat ttactccgag gagaaaaaca
     2581 gagtatttct acaacagtaa ccaattttac caatcaaaaa gaagaagtcg tcgtaaattt
     2641 ggaagctaga ggagttaaaa tagatggaga acaaactaaa aaaattactt taaaagctca
     2701 tggaagcgaa gttgtatcct tttcagttca ggcagaggag gtaaacaata ccaaatccgc
     2761 tagtttaaaa ttttcaataa aaggtaaatt tgaagatagt gtattaaaaa aagttcctct
     2821 taaattcttt ggtttaaaaa aaatagagtc ggttgctatg aaaattccta gcaatggaca
     2881 aaaagataag gcaaccctaa agcttccgaa tacagcgacc gaagagcagc taacgcttcg
     2941 atttcatcct ggtaacattg aagccttgag agcaagtcta ccttatttaa tcgattatcc
     3001 ctatggttgt gttgaacaaa ccatgagtag atttgttcca gttctagccg ctcaaaaatc
     3061 agttagcctt tctttaaaac agagagacga gctacctaaa atggcagcac aaggaatcaa
     3121 aaatttaaaa acacttcttg gatcagaagg gggatttaaa tggttttcta gagatagtct
     3181 agaagattct atgatgactg cttatgttta cagggcactt tcgattgcaa aaaaattgaa
     3241 tacccaagtg ccagaagaac ttttaaatca atctagaaat tacttatata aagtattgga
     3301 aacaacgtct gatccatttc aaaaggctta tattatattc tctctatcag agggaggtga
     3361 agtagagaaa tcactaatcg actctttgat aagtattagc ggaaaacaga agttatatgg
     3421 aaaggctctc actggacttg ccgcattcgc aagtgggcaa aaagaccaag cgcaaagtat
     3481 ttataaaaaa ataatttccg aatcaggaat ttataaagga aaaattccaa aatttgaagc
     3541 ggacattgaa aatgatgata tcgagacaat ctctgcaatt ctactccttg cagttcgact
     3601 taatgaccta gaaatttctg aatcattgag tgaagaactc ttaaatagaa aagttgatat
     3661 agcttggaaa aattctagag atactggaat ggcagttctt gcccttgctg aaaaattaga
     3721 agttttttct gagaagctaa ttccaattaa cctacaaatt aaagtaaatg gtaaagattt
     3781 caaatcaatc tctataaaac caacagaaat tctatctggt aaatcagaat ggaacttcaa
     3841 tgtaagttct attgcaaaag gggataatac catagaactt atcaaggaaa agggaaacac
     3901 ggtatacgca attgcttctt tagaatatgt agacagatct aaaaatttta actcctttga
     3961 aaaagggatc aaagtgaaga gagaatattt ttctgttttg cccgaagaaa agtctggtaa
     4021 aacattgtta accgctaaaa atgcaaattc attcaaacca ggagaattag taatggtctc
     4081 gatcgaagta gaaagaagcg gcaaaagcga tacctatttt atggttgaag atccactcat
     4141 tcctggtttt agctttgtaa caaaggatac aaactactac ataggtgaat actctgctga
     4201 ttatgaatct agacaaatct atgacgatag agcagtattt tttattagag gtcctgcaca
     4261 aaaaactatc gtcagatatt ttcttagatc agatatgtca gggaattaca atgtgccccc
     4321 tgcaaaagct agtctcatgt attatccaga gattatggga aattcttcct cacaaaattt
     4381 aaaagtggag taaatgatga attatctaag atttcttttt tttctattat ttataaatgg
     4441 atttcaagca ataactagtt ctgaagtaaa aattgaatca ccaagaggag gattcactac
     4501 tgcaagagta atcgaaatta caggaacggt agccgataca agcgtggaaa gaattacatt
     4561 agtaatgaat ggagttcctc aaactgtgaa agttcagtct ggaaatttta gaataaagtc
     4621 agtcgttgca cccggaagca attctataga ggtcaaacat ggtaagcatt cagatagagt
     4681 ttcatttttc gcaagtgttc catcaaagga tattaaaatt gttctaactt gggatacacc
     4741 gactgacgta gacctttggg taatcgatcc caaaggagaa aaaacttatt atgccgccaa
     4801 ttcaagttca tccggtggaa atttagatgt ggacgttaca gatggatatg gaccagaaac
     4861 attcacaatg gcaaaagctt tgtcaggctc ctatgctgta aatgttcaat attattcaaa
     4921 taataacatt ccaatcacga gggttaagtt atatgtaatc ttgtatgaag gaacatcaaa
     4981 agaagaaaaa aaatactttg aatttactat gacaaaaggt cagcaagttt atcacattgc
     5041 cgactttcag atagaaggcg actaataagc tattttgcat aaatttataa tcatttgctt
     5101 tttctctatt tcattttcac tttttggatt tgatgaagtt tacttaaatc aaattataaa
     5161 agaatgggag gaaagtaaaa aaaacagtgc aattgtctta attacaaatc ctttttcggg
     5221 taggatagaa ttcgtttaca atgcaaaaga tgccttctac aagaaattac ctcccggatc
     5281 gattctaaaa ccactgtctg cactaacgct tctttctcaa aatgatacaa aagataaaga
     5341 tacatccttt atatgtaaag gaaagttctg ggagccaaaa gaaaattttt ttactagatc
     5401 cgactataaa aattttaata tattagaaga taaagaatct ggtaaaaaat atttaaaatg
     5461 ctctacagaa gctggtcatg gagaaattaa tttaagagaa gcaatttctt actcctgtaa
     5521 tgttttcttt ctaacaaaga tattcaagtc taaaaacctt ttttattcct catttgttcg
     5581 agattggaat ttattcgaag gaacaggtgc ttcatttgaa agaatttcag tttctaattt
     5641 taatgacaaa attccaaaca gccctcttga atccatgctc tccggcattg gagaagacgg
     5701 taaaataaaa gtaactgctc tcaaagttgc gcaaatcttt ggggcaattt ttgcaaatac
     5761 tccgttctta aagccaatac aaaaaggaga aactcccgag caagtctatc catttcctta
     5821 ctctgaaaaa tatagaaata ttattttaca aggacttaga gaagctgtaa aaaatggaac
     5881 gttaagtgat ataaatctta tcaaaaataa cgttcaaatt ctaggaggga aaacaggaag
     5941 tcccactaga gaagggaaaa aatactccac acatggctgg agtattattt ttttttaaaa
     6001 agcagaatct gcatatttat tagtggcatt tacacaaaaa ggatcgggga gaaaagatgc
     6061 aatacccttg tcctcattaa tcttaaatgc attctaattt ctattttcta ttactaacta
     6121 tgaatacgcc tagaataaca aaaatggttc caattatttg caataaattc agtgtttccc
     6181 ctaaaaaaat ccaagcgagg aaaactgtag aaacaggacc gatagttcct ataatcgaag
     6241 cgactcctga accgactctt ttaatccctt gagaaagtaa aaatgttggt aaaaccgtcg
     6301 atataattgc catcaaaaaa cttaaaacat aaacttctga tggcaattgc aaactctcta
     6361 atttgtgagt aaaaaagaaa tgaattgtaa cagagagaca ggcaactaac atagcataag
     6421 cagtaaaccg agcggctcca atcctaggaa tcattctacc gctcccgatt aaataaatcg
     6481 catacgtaaa agcacaaaga aatattaaaa atgctcctaa ataaatatgt tcagtttttt
     6541 caaccttatc tgcaaaaaaa acaattagta ttcctatata tgtaatagct aatgatatta
     6601 tttcccttga acgaattttt tgcttaaata aaaaactgga aagtaaaact acaattgttg
     6661 ggtagataaa taatattaac cgctccatac ctgcactaat gtaaacaagt ccaataaaat
     6721 ctaatatact agaaagataa taacctaaaa agcctaagag gataacaaaa atccaatctg
     6781 aaatttgtaa gtttgttttt tctgattttg atttatgcaa accaacaact aaaaaaaatg
     6841 gaacggaaaa tgccatacgt aaaaatagca atgtagtggc atccactcta taataatagg
     6901 caagttttat cataactgct ttcgcagaaa atccaatagc agacaacaaa acaaaactta
     6961 ctccaactat ataataactg cctctttctt tcataatttc attaattaat tatatatatt
     7021 tccctacaat tggatttttt ttcaaaatct ccgcacataa attgaatttc ttttctctct
     7081 ttttatatga tacaaataaa atctaagaat tcatttaaaa gcccaaaatc atcctttcaa
     7141 gagaataaga ccgtctctac cttactcatc ccaaattatt taatcgagta tcttcattgc
     7201 cgtgtaaacg aaaatggtag tttagcgaaa tacctaaaat tgcttcttac taattgtaga
     7261 gtctttactc attcaggtat gctacctcct ccagataaaa taaaaacaga ataccaaaaa
     7321 gaaggtctcg acctaacaaa ggtaaatttt cgtgttgata atgatgactg gattgaatta
     7381 ggtgaattgg cattagcctt tggtaaatct cgttgttatc tgtttagcta tttggtggag
     7441 ttagatctac taggattttg ggatgctctg tctaatttta atatgaatct tggagctcct
     7501 acacacccaa agctatcact aaaagtcagt tggaaattaa accgagccaa gtatagtttt
     7561 aagcgagcct atcatgtaaa aatctagaaa catataaaaa aagaaatcaa aaaactactt
     7621 ttaagccaag gtttgctcca aagcctttct caatgatagt cgcttctgct agtaatttaa
     7681 gaatagcaaa attaaattcc attccggcga taaagtaatt tgtattagtt tctagtttta
     7741 cttttctacg aaagtccata gctagactcc cttctggatt cattgcccca tttgttggat
     7801 tcagtgcgtt attcgaaaag tatttataat aatctgaagt taaaagagag ctattataaa
     7861 tataggttgg atcaagagaa agtttgatcg gacctcctct tgataaatga agatttgctt
     7921 ctccatagtt tttgctaaaa ccgacaccac aaaacaagtc aaagaagtaa aatattttta
     7981 gacctgatct taattcaatg ggtatactat gaatattaga agagaaatcg aattctgttt
     8041 ttgcaatcca tgatcccttt aattcaccaa agctaacagt ttcatttgtc ctaggttgat
     8101 gattgatttg catatttaat ctttgagcgt tatatcctat acccaaggac aaaccagaaa
     8161 agctaattaa agaatagctt gatattttag gttcaataat atgatatctt aaaatagcgc
     8221 caatgctttg gacttgaatc gatcctgaaa tttcaggctt atttatgtaa gcagctttta
     8281 catcagtagt attataacca acattcattc catgaaaaaa taaattaaac ctatggagat
     8341 aatttttatt atccacgtcc tcattttcat catcatcatc ttcattcctt tgctgaaggg
     8401 catcgccctt tccaataaac caacccaaat taatagcgcc cataatggtt ggagcagaag
     8461 cgattcccgc atttggaagt tttggaatag taatatctcc ataattaata gttatatcat
     8521 ctttttttat atagcttgca gaaagaccac ttcctagttg aaaacgattt acatatccag
     8581 atccgatcat agaagaatta ttatttataa taataccaga ctcaatcatt gactccatga
     8641 caggtattaa atattgagtt tgaaatgtag aatccagatt actaaattca gaaagatatt
     8701 tctgtgggat cacattacat gcctgaccca cgcatgttgc agtagagaaa acgttagtag
     8761 aagaatgaat gaagaaaagt aagcaaaatg gaagaaaatg aactcttatc ttttcaatta
     8821 gaaatcgaca acttttgagc atagcgcaaa actatagcag aactcaaaag ttagtcaaga
     8881 aatttagtaa attatttatt tcttaggtgg aaatttatca ataaggaaat aggtttccat
     8941 tagacctttg ccttttatat cgatagctcc tctcttctca aaaatatatt ttcctttcaa
     9001 tttatcatac acttgtctag aagtatgtat catttctggt aatccatatg attccattcg
     9061 actagcagta tttactgtat ctccccaaat atcataggca atcttttcta ctccaatcac
     9121 tccggcaaca gcaggtccgc aatgcattcc aattcgaatt ttaaaatcgg gatatttcat
     9181 aagcaaatcg attaattctt gctgatggct aaacttcaag agatcaagtg ctaaatctgc
     9241 cattgcttct aagtgattat caattgatcc aggcagccca ccaacaacca tataggcgtc
     9301 tccaatcgtt ttcattttct ctaaattgtg aaccattgtt aagacatcaa aatgagaaaa
     9361 tacgttattc aataaaacaa ttaaatctgt cgcatctaca ctttgcgaaa gttttgtaaa
     9421 tccaaccaca tccgcaaata aaatactagc aaatggaata ttgtctacga ttaagacttc
     9481 atcatgcgtt ttaattcttt caattacatg agtaggtaaa atattttgca ataatgcttc
     9541 gttctgttta ttagatcggg acaattcttt cgttctatct ttaactttct tttccaattc
     9601 ttggtttgat tcctcaagag tttgaattaa tttatcttcc gcatctgtct ttgccttttt
     9661 taaaatatta attctatcgg ctaacgcgaa agaaaaaaat gccacttcga acgcacctgt
     9721 aaataaacta aaataattta aaaatcttaa atcatctaca aaaccaaata acctgaagat
     9781 attgaatgtt ccaacaatag acgctatacc ccatccaacc aaataaagtc tagcagcttt
     9841 atttccgtaa attaaagaca ttataccagc agcaattaag aacgggggat agatcaatga
     9901 aaacgaatta ctaattataa ttggaagatt tgaatctaaa ataaatgaaa ttatgattac
     9961 aggaattgat aaaattctaa gccataataa gattttattt agaattggag aaaacttttt
    10021 ggtatccaaa aactctacgg taaaaaaatt caagcataga gtcatagcaa ctccgctcac
    10081 atgaataatt ctattttgca tatatggaaa ttctgaccaa aaatacttaa aaccaaatcc
    10141 atgaaatgaa atcatgtaaa gtatgctgaa aaatacagtt aaaacataat aaatgtaaat
    10201 cttttctttt attgatataa atagaaatag attataaaaa agcattataa tcatgatacc
    10261 aaaatacaaa cctaacagca tttgtgtttc attgtcacga ttatagaaaa gtttttgagg
    10321 agctaagtat ataggcaatt gaactgcact aattgagtga atcctaacgt atattactgg
    10381 atctttttga atctcgaatg gaactgtaaa atttagaacc ctactttcga tgtctcgctt
    10441 tgtaaataca aaatgatctc caccctcttt ctcaatatat acatccgcct cttcttcctc
    10501 atgatcaatc ggaaaataaa aattaacaaa atctagttga ggatactcta taaaaaaaac
    10561 ccattcatca tttaaaaaac tattttgatt tttaatttga aatctaagcc aataaacaga
    10621 ggaagtataa ccaaaattat atttatttac atcatttctt acaaagtctt tttctaatac
    10681 ttcttcaaag cttaaattac cggtcttatc ttcaaaaaac tcagcgtgag gaattacatc
    10741 ataaatctta tccatgtaca gaaccaatgg attctttgta tcattttcat cttctgcata
    10801 agcgttagaa tgacagaaca caagaattat aaaacaaata gaaataagtt tcttgattag
    10861 aaatttgatc atacactata cattcatttt attttcttag aaaaactgtt cctgcaattt
    10921 ttggttctct taaattttcg gtgaactcac actgttaatt cactgtaata tatcatttcc
    10981 atctaggttt ttccaccttt ttcacgtcct aaagagaaat tccctgatat tttttcaaat
    11041 tactcagctt cacagtctat ttatacttat tttttcataa aaaaaatgat ttcacgcctt
    11101 tttatctcaa ttttgactga attcattact tttctattcc aaaactgcaa aattttagaa
    11161 gaattaaaag aatgctaaag agtttttgaa tccaattttg aatgagcaga ttttcctaat
    11221 agaagatgta acagataaaa caaggtataa gagacatagc aaagcatttc tattttagag
    11281 taatatccgc aagctgtctt ttccattttt tcaaaaaaaa tcgaaatctc tatagaaatg
    11341 cgaatctaat taaatatata taagtagcac aaatattgca ttattaattc ataatagaag
    11401 tcttttaatc tttatctaca aaggaagttg aacgcatgag cacatcgtat aaaaaaaaca
    11461 gccaaacaat tgaccttact tgcagcagca aaggtaattc ttcctatctt ttaaaagtta
    11521 ctgtatctca taatgaaaaa gggattattt ataaagtgac ttctgtatta tttgcgcatg
    11581 gctgggatat tgaagaggcg gttgctgaaa ccgtcgatgg ccgtctcgtt aaagatgttt
    11641 tcctaattcg aaatttagaa ggcaaaaaac tatctgagca agcctttcaa aaaatacgct
    11701 cagatttaaa ctctcttttt tatgaaggta attcggtaac tgaatatctc caagcctctg
    11761 ataagaatgt aaatgctttt aaaaaaccat ataactccac agttaatgtt tttaatccac
    11821 cctcgattga attcacagtt ctcgatattc gcactcttga tcgtccaggt ttactctatg
    11881 agatttctca aatattatac ttatatgata tagatatcat ttcttttact gcaaagagtg
    11941 aaaatcacgt catccgcgat agcttcttaa tatgcactcc aaatagtcaa aaactttctg
    12001 aacaagaatg caaccgacta aaagacaaac ttctttccat tttaagtcca aaataattta
    12061 ctcttccggg tgcttaccta gttcaaaaga attaataaat tcatttatag tattaatatg
    12121 ttcttcttca ggggaaagac catcttcacc tggatcatac aaatgctcgt caaatacatg
    12181 aaaatcaaat attcttaatt caaattccgg tagcattatt atgatattct cagagtgcaa
    12241 atctaaaatt aatttttcct tttgcgctaa atagcgagta gcctcaatta ctctatgaaa
    12301 attaaatgct atttttttta attttgaaac aggcactttt ccaaatttat gtgtatcaaa
    12361 atttaatttc cattttggta gaaatctatc ctcaagtgtg ctctgcttta ctttttcggt
    12421 taactttaca aattctttta agtgctttcc aggaattaaa atttgattat cacagggtgt
    12481 aatcgttgtt tgtggaattc ctatcggtgt ccttctactc ctaagtccca taaaaaactt
    12541 tgttggtaaa accaagtccg gaagtaatgt cttaactttc caataatgaa gcctttccaa
    12601 tccaagtctc ttaaatttaa aatcaagtaa ttcctttgac gaatttttaa aacattgttt
    12661 ttctaaataa ttcttcaatt ctagctcttc tggctttaaa tacatttcta aattttttga
    12721 tacttcttta aaaagagatc cgaatagtgg atctgatcct aacttcgact tacctacctt
    12781 tacaacctga ttccaaggaa gtctgtaaac aaatttatat ccacctcttc caatgaaatc
    12841 gtcattagag attggcatga aattctccat aaacacgcca ttgaacttat aggttacttg
    12901 aaaaacatgc gttactggaa atatcttctc atatactttt cttacaattc ctgatttccg
    12961 cagactcaga tctattggca attcttcaac aggtataggt ggatcggtct tggtatgatg
    13021 aaaggataat tccttttcta aacctttttc taaaagctca ataaaatttg ggatttgatt
    13081 aaacttcttt aaattaaaaa aatctttaac tttgttttta taattactaa atcttgattc
    13141 ttcatccata ttttcttacc gtatccaaaa tcccatcgcc aaatttttct attttcgcat
    13201 cccctaagcc tttaatatga attaagtcct gcttggtttg tggttttaag tttgcaattc
    13261 tagtaatcac tgaattatgt aaaaccataa attttttcca tctatatttt cttgcttctc
    13321 tatctctgta gttttttaaa gcttttacta aaacttgatt tggggtttga atttttcttg
    13381 ttcgtaaacc agattgaatt tcagattcta ttttttcttt tctagtcatt ggaggatgac
    13441 tagaaagata gattttagga tatttctttc ccgcaatctt taattccctt ttttcaatcc
    13501 attcttctaa ttttgtaaga attgcctctt caggaatagt gcttaattta ccatagaaag
    13561 atgactgatc tgcttttgat cgcaagatat tctttgcctt agatcctctt agaattcctg
    13621 ctatcatttt tttcccatac actgctggag cagaaatgag tagacttcga attatattta
    13681 attcatctga ggcaaattca tatttacccc tcagaacttt cttttccagt tttaattgca
    13741 actcttctaa ataatcatta tgaggattag gtatattggc tttgcaaatg tcgcatatcc
    13801 cgcatgtttg aatttcttcc ccaaaatagc tgcacaaaaa tttctgtctg catacattcg
    13861 aaaatgcata ggcttttatt gcatcaagaa gtgtatcgcc tcccttatgg ttcgactcct
    13921 ttcttaaaat aaaattttga attcctagat ctgcattctg aaaaaacaat atacatctag
    13981 aattttttcc atctctaccc gctctaccag cttcttgata gtaagattca atagaagaag
    14041 ggatttgata atgtaaaacc aatcttacat ctggagagtc aagccccata ccaaaagcgt
    14101 tagtagccac aagtatttta accttaccag aagaataggc atcttgcgct ttttcacgca
    14161 ttgaatctgt tttacctgca tgataacgag ttgctttaaa cccattttct aatagaaatt
    14221 tacagacttc atccacttta tttcgagttg cacaatagat tatcactctt ccgtgattct
    14281 tcgttttaaa ttcagaattt aatatttcta ataattttat ttctttctcc gtttcattcc
    14341 taagatactc tatttgaaaa gataaattcg gtctttgaaa actctttttc acaatataag
    14401 aattctttaa attcaaagaa ttttcgatat catttaaaac cttttcagtg gctgttgcgg
    14461 taagcgctat aaaaggagaa tttggatgta attgcctgag tcgatacaat tctcgatact
    14521 ctggtctaaa gtcatgcccc cattgagaaa cacaatgcgc ctcatctact gcaaagaaat
    14581 ttactttcat tttcttaagc attgttgtaa aataagaaga aagcgcgcgt tctggtgata
    14641 tatagaggag tttgattttt ccagtaacag ctcggctaat agctgttagc tgttctaatt
    14701 catcttggga agaattgcaa gatgatgcag aaacaccaat cttattcaaa gactcaacct
    14761 gatctttcat taaggcaatt aagggagaaa tgacaatgct tactccttcc ttttgtatgc
    14821 ttgcaggtag ttggtataat aatgatttac ccccacctgt aggaaaaata gaaatcacat
    14881 ctctccccgc aagcaaagcc tctattgctt ctttttgccc ctctctaaat tgtttgagct
    14941 taaaaacttc ctctaattgt ttctgtatac tttgcaagat cttttgtttg attaataaaa
    15001 ctaaattacc ttaattaact caccttacat tatcattgct tcataaaaac aaagattgaa
    15061 ttcgtgcaaa ccaaacgttc aaacgtggtt tgctcctatt gtcattgaaa aatgataata
    15121 ggcttgaaag agaaggaacg aagagacagt caacccatcg ctttataagc tctccaacag
    15181 ccaagctatc gcagacaatt gattttttta attcattcgc attctctaat cttataaata
    15241 ccatcgttta ttcttttttg cgcaaagtga gttaattaat ttatattatc attctcatta
    15301 attcacatct tttatgcatt atctattaat taagcttcta ctttttacct gcaattctat
    15361 taattatcca aaggagtgta agagcaccta ataaatcgga tattaaactc gaaattattc
    15421 ctgtcgaggc aaatccaacc aatgacaata atagaccacc aatcaatgat ccaattatac
    15481 ctatgatcat gttttttaca aaaccgaaac cacttcctga gtagatacga ccggcaatat
    15541 atcccactaa cgcaccaact aaaaccattc caattaattt aaataacatt tttattctcc
    15601 tattttattt ctgcaatttg aaagtcagca attgtatcag gcttagatgt tgccggagtt
    15661 tgctgctctt ttgttaattc tttaactctc tctgaaatat ataaatccag catattaatg
    15721 gtaattttgc cttttctgga atagtctgcc tttccattca atccttctac taacgctttt
    15781 gtaaacgctc catttcccca actcttatcc tctaaagaca gttgattttt tgtagaggaa
    15841 gtaaatacta caattccatt ttctgtatct gataattcgt taattaatat tgtaatatca
    15901 tttccttttc caaaaacgtt cgcagaatga caggtatccc caaaaaacac aaccttcccc
    15961 tgtatagaag ttaacgtatt ttttatctct aaatatgaca cccctgtgct tttaaacttt
    16021 tcaggatcaa agttatgcgg aagaaaataa taattcccaa ttgagtcatt tacaccatgt
    16081 cccgacataa agatcattgc gtagtcattt gctttagttt ctttttgaat ccactctaat
    16141 ccatctaata tattcgaagt cgttgcttcc ttatttaaaa gtgattttac aaagatatct
    16201 ttatacattt ttcctttttg tctttgcatt gtattgataa aatcactcgc atctttcgct
    16261 ccaaattgaa gctgtaaact agaatcttga taatcactta ctccaactgc taaaataaaa
    16321 agtcttggct tttcttggtc ttgctgtaat ttattttccc aatcaatttg caaatttgct
    16381 ggttcacttg aattaaattt gctctgtgcg attagactaa tcacacaatc tcttggaggt
    16441 atatcaattc gaattgaacg agcaggactt tccttttcta cagcaactaa attcatacct
    16501 ttggttccag taatgggtct tccatcgatt aacacccgta tgccgcttac aggatttccc
    16561 gatggtgttc ttactgtata ttgaataaat acacttttat taccagtttt atatatactt
    16621 ttaggtgaga taatttcaat gacgggtgga aaatgatccg ttattgttcc agtatctttt
    16681 tttctaccag attcattatt tgtttgctga attgtttcct gttcacttcc aatatctaaa
    16741 attctatcaa taatatcagg tctataatac ttgcttctaa actttgctat tggaaaaaaa
    16801 tcagctgaat gatcctttcc tctatttaca tgccatccca aaaattcctc tgttcctggg
    16861 gctgtatcat agtaacctga agctgtgcta attacccatc tctttttatc cttatgtaaa
    16921 aacaaagaaa gtaactcagt tccgtcctct gttttatacc accgaatcgt tccatctcca
    16981 aaagaggcaa ttgcatattt accgttagat gttagattca cattccatgc aattcctgga
    17041 gctggaactt tccaaagttc tgttcctgac ttatcgtaat aacgtaaatt ccattcggtc
    17101 cccagaataa acttatcatc ctgaattgcg agacatcgag atgtctcatg atcttctaat
    17161 acaattcgat ttccttttaa taaaggagaa taattatttc tccagtttgt aatccgaagt
    17221 gaacttgtat ctggtttata cattccatcc gtatttaatt catctaaata tactcgtcta
    17281 tctgtgagag agaattgtgc ctggtctttt ccaaattgta aaaatccaaa ttgaactctt
    17341 ttcccatctt cagatacaag aaatttttcc tgtgcatctc gatagtcagg agtatcctga
    17401 agtttataga ttaatcttcg atcctgtgaa tctaaaatac cataagatgg atcgtgagtt
    17461 gagtaagcaa ttcctccatc ttttaatgga ataacatgca tgatcgtatt ccaagaagca
    17521 ggtagatctt tataatctcc acgtcctcca ttcgaccatt tacgaatgaa atatccattc
    17581 ttttgagcca ttccacctgc atataaatac tcaccatcat tcgaccaagt aaccttactt
    17641 agatccccgt ttccaattcc actaacgctt ggtgcaaaag aataattgat agtatctttt
    17701 tctatatcaa aaacctcaac agtcgttgtc aaatcatagc caactgcaag ttttttccca
    17761 tcacttgaaa cacgcatcga aattggaata cgcgcatttt gtccactttt cctgtctaga
    17821 agtttaagtt tattactctc aagtgtataa tatcgaataa aaccatccag gctaatcgta
    17881 attatccgca agcttccatt ttgtaagata aaccctgtat cataacttct atctctataa
    17941 tccatatctt cggctacaag attaaagtta atgccattat agattttaaa tcctaatttt
    18001 aaattgacgg ctaaatattt tccatcgggc gaatatgcca tgtggtaaat aatttcatct
    18061 accttcgata tacttctttt taggtttcca gtctccacat caaatatata aataaactta
    18121 ccatcatttt cttctagacc agtccaacca gcaactgcca atgttctacc gtcaggcgac
    18181 aaagcaacag catttacttt cccctcatgt ccagatgcaa ttggaacacg aatagttctt
    18241 agtagtctgc cagtggagag ttcccaaatt tttgctgttt tgtccgttga agcagtagcc
    18301 aacaatctat tctctgcatc cgttgaaatt ctacgtatta cagctgtatg cattccagct
    18361 tcgactttaa gaattggatc ttttcctatt ggatcagcta aaagttggaa gcagctaagg
    18421 agtataatgg taaataaaat cttcatagac taaatcctaa tcaattaaaa taaactacat
    18481 cgatatccgc ttcattgatt ttctgctcac cttgttctga gataagaaaa tattgattgt
    18541 ctagttggta aatagaccca gtgataacac ttccattttt taatttaatc ttctctggtt
    18601 gccttataac ttgagcccta gcattggagc tatcttgact tacttttctg ttttgaatta
    18661 gattaagcaa atccgatctg tcccaagtat tcctaagctt agatccatca attccaatta
    18721 aactttcaga ttcttctcta cttctcttaa cagaagattc ttcaattcct tttttcagct
    18781 taggcttttc tgaacattta agatttactt cttgaattga ggtagttgag tattcttttc
    18841 tcttagctcg tttaactaga ttctcagcat ccattttctg caaaacatct ctttgattct
    18901 tcgggcttgt ttcaacagaa tatccatacg gaacaagttg tgcatttctt tctaaatttt
    18961 ccgttagccg cttcatttca attgtatctt caatcgttgt atcgtcttcg gcttcaggag
    19021 ctctaattct agtttgaaca aatccttctt gaacatttaa ttttgtagaa ccgtcatttg
    19081 ctacactagt atcaaaaatt gttcctctta ctgcggcaat tgcagttggt gatattacct
    19141 cgattctttc cccgctaaaa acttttccaa ctttgaacaa agcagatcca aactttaaaa
    19201 aaccagaata tacattttca tctggctttt ctttaaattt aagagagaaa acagaatttt
    19261 ctctaactcg aattgtaaag tttgataaag attgtttcac ttgtagatcg caataggatt
    19321 ttttaccaac aaatatggtt tgattttctt ctacaatatc acccacattt atttttttat
    19381 ttccaactgt tacatcaccg actagagcta aaatcacacc agaagagcgg ctagcaacga
    19441 ttttgcgctc agtcccttct aaaaattctt tgataaatgt atttcctctc gcacctgcca
    19501 tgactgcagc agtccaacca tttgtatttt ttgcaaagac atctgctttg ttatcaacta
    19561 aaaacttaac aacagggaag tgattattat tagctgcaaa cataagtgcg cttgttccaa
    19621 gagagtctct aaaattgata tctgcatttt gtgctagaga ttttttcaca ccatctaagt
    19681 caccgatact tgccgaggag agcaattcaa tattcggatc agcataaaca gtaaaagtca
    19741 tgaaacaaag gaggaagaaa agttttaatt tgaatctcat ggtaaacctt catcggtaat
    19801 ttaactctaa caaatacatg actaaaatta aaatggtttc ctaaattgca ttttaaaatc
    19861 gttaccagtt gatttttaaa atatcccaag gtttgcctat tttaaattca tagccagcaa
    19921 tgctacagta tattgttcaa tgaatccctc gtgttagctt ttgtaaatag caaatttata
    19981 agcttgattc tagaatctga caaaaaaggg ttgccaatat gctcgagcat ttttaggtta
    20041 aaattcgtta accatatacg aggaaaaatc ataatgaaaa aatctttatt tgcgctagta
    20101 ttgcttgctc cttttttttc agcatgccaa accatttcta aatttttcgc gagtgcgccc
    20161 ccacctccgc aaccacagcc tgcttatgtt gctccaagaa tctatcctga gaaaataaat
    20221 ttgagcgaag taaaggtaga agctatcaat gaagctccaa acgaacctct tggggtttat
    20281 aaaggctctg atggaaataa ctacctcttc ctaaacctaa tctatgaaaa ttcaggacta
    20341 tcagtaattg aagcaattaa aaacggagag attttagaca atatgattat tgttcgagat
    20401 caaactggta agattattgg ttcagacgtt gttaactttc aggcatttgc aacgatcgag
    20461 aaaaaactat ttaaaaatac acaggaaata gttagaacaa aagatggatt tgaaacccct
    20521 gttcgtcctc aaagtgcgaa aagagatcta gagcctgaaa tttctacttc ctaccaaagt
    20581 aaaatttaca cagagaaaga aaaagttccg caagtaatca aactcaatga gcttcctgct
    20641 aataattctt atatttcaat caatctagag ttagtgtatg tagaaccgtt tatccaacaa
    20701 atctgtgata aaaatggaaa gaatccatgc acatacgtag atgaagttgt aactaaagaa
    20761 gtgcgtgaag caaataaaaa gaaattagaa gcaatcattg ctgaatatca aaaaactcct
    20821 accgacaaac aaccagcaga aaaaagaagt cctaaaaagt ttatcttaac tcctgctgaa
    20881 atccaagctt tcaaagacaa aatgaaaaag aacgttagta aaactggcat tactttagaa
    20941 ggagaaaaaa caaaggttgc tgattctgtt gttgatgttg aaaaagcaaa actaaactac
    21001 agagaatgga gtctagtaag tgcaccaaag tattttcaag tgaatacaga agtcattaaa
    21061 ccgttagtag gagaagcacc atctgcaatt ccaggaaata tcacacctgc acctgtgaat
    21121 ccaatgaatc cagttactcc taacaataac acaaattcaa ttacaaatcc tagtgcagat
    21181 ttgccaattg aaggattctc cgatacggtt actcttaaaa atggattgat ctttaaaaat
    21241 gtaaaagcta ctattaagga atccactgtt ttaattacat cacaagatgg aaaaacaatg
    21301 gaaatcaata agtccgaatt agttagtata aaaaaataat acaatcctac tgatctaaaa
    21361 gaacccacag acaaaacatc tgtgggtttt tttgttaaat cttagcagaa tctttcataa
    21421 tacccatcat actattataa ccaagaacga ctgtcgcagc ggcaaaacca cccattaaag
    21481 ctccagaaat tccaggagaa caagcatctg ctcctgtaat aaaaacattt tcaatcggag
    21541 tctttactcc catccattct ttcgtatatc gttctaatgt gcatggaatt ccataaatag
    21601 ttccagcttt atgatctgta aaatattcat ttgtgagtgg cgtcgacaat tctttatact
    21661 caacaagatc tttatatcca gggaaatgtc tctccatgaa atttaacatt ccatccgcta
    21721 tacgatcttt taattcttta taatccgttc ctctgttttt ccactctcca ttcttccact
    21781 ttgcaaatgg ttcataattc acaaaactaa ttacttctcc agtaaacgat tctgcctttg
    21841 ggtctttcag agatggaaaa gaagcataga aagcagttgg ttctcctttt aaaagattat
    21901 ctttctcatt gaaatttttg tcatgatcta tggagttata aatccaataa ttttctcctt
    21961 taaatccaag cttagccgga ttttctttga aacctaaata gagagttaca ctacttgctc
    22021 ctgttacaaa attagaaata ctttctctaa aaggtatttc tacattctgt ggaattaatt
    22081 tggtataggt attatatact ccagcatcag aaataataat tggagcaaag tattctttct
    22141 ctaaaatatt ttttcctttt gtttgtttta ctttaacacc gattgctttt tcgccatcca
    22201 ttataatttc tgtgacttca ctgaatatat gcatagctcc acccttcttt tcgatgattg
    22261 gcttaacgct ttcagctatc gttcccgccc cgcctacagg atagaaacct ccattcagat
    22321 aatgaactac tagtccagca tgcatataaa atgcactcgt agatggaggc agtccgtaat
    22381 ctccccattg agaaacaaga acagctttca gaactggatc tttgaaattc gcatccaaat
    22441 actccttagt tgtgatcatt ggtgatttgg caccagttaa tttaaaatta tttgattcag
    22501 tatctaaaag cattggtttt gcttttaatg taaattgctt agttgcccaa ttagaaaaag
    22561 acttaacatc ggaaaaataa ttttctattg cctgcttctc atgtggaaat ttttggatca
    22621 aatcagatct aaatttttct tctgaactgt attgatcaaa tgttatttct ggataaacaa
    22681 atctttcaaa tagatccggc attggattcc aatccacttt tccttctgtc actatatcaa
    22741 atagctttcg aagcatggat tgcttgccca tgtcccccac ataatgaatt ccaacgtccc
    22801 aaagaaattt cccttctctt ttaaatgtat gagtaaaacc accaagttta aaatgctttt
    22861 ctaagactaa aactttttta tttttaagtc gtgctaaaat acttgcagtc gtcagtgctc
    22921 ccataccaga accaattaca atcgcatcaa aatgattttc tttttccatt gactctttcc
    22981 atccttatgt aaatttgttt aatgttgaca ttgtaaatat taatgttact attgtcaaca
    23041 ttaatatcct ttatataggt ggatttcatg aaaaaaggta agtcgagttc aaactatcat
    23101 catggcgatt taaaaaaagc gcttctggat tctggtgtgt ccattttagc agaacaagga
    23161 ataaagaatt taagcttaag agaagccgca aggcgcgccg gtgtcagtca taccgctcct
    23221 tatcgacatt ttgaagataa agatgcgtta ctcgccgcca ttgcagaaga aggttttaat
    23281 cggttaattc taaagctaga agaaggaatt gcaaagcacc ccaataatcc attgcaacgt
    23341 cattatgcaa tcggggaagc ctatgtccaa tttgcacttg aaaatccaaa tcacctacaa
    23401 gtcatgtttg gtaatgtctt agaagactat aaaaaatatt ctagtttaaa acaagtctcg
    23461 gacaaatcct ttagcctatt agttgaaaca atcaaagatt gtcaaaaagc cggtattgta
    23521 aagaaatcaa atgcaattca actcgcactc gtagcctggt cagctattca tggactatcc
    23581 ctactcacaa ttgaaaaacg aaataaatgg atttctgaga acaactcccg catcataaaa
    23641 attctgggag atgttctctt taacggaatg aaagcttagt tctactatca tattaatcta
    23701 cttgtcgaac tactttaatt gcagtggttc caagtccgtt accaatagca atatagatat
    23761 aattcttatt tgtaatttga tcatacaaag atacagacgt catgatgtat tttgagttag
    23821 atcctaatcc atttgttcct tgttgaaccc aacctgcgcc actcattgtt ccactggttg
    23881 cagatggagt cgttcctgag aatttaaaaa ctcggactcc gctgactgga tcatcaaagc
    23941 ctaaataaag attattaccg tttgcaatta acatcccgat tgccgttgcg gtgctaccaa
    24001 tatctgtttc tgttccacta attatttttg accaaccagc aggcgcacac tgaccacttg
    24061 cattcggtgt gcaagtccaa acctctcctc ttaaattggt cgtcggtgtt gtatcaccaa
    24121 catttctaac catatataat ttcccattat agaggataat ataaggatat gctttttcac
    24181 caggccttac ttttcctaaa ccaccaccag tcgtggaatt agacgctggc atatagagag
    24241 atgttcctgt cgctgcactc ggagtagata aaacagggtc aaatgtagtt gtccctaatt
    24301 tccaatcgtt tgcgttacct ggtgttgcgc ttaaaatttt actagtagta acatagatta
    24361 caccaccgtt attcgcaaga taagtagatg cgccttgagt aaaaataaca tcaataccaa
    24421 ctaccccaga actattcgga tttgttggtt ctcctgcagc aggcgctttt ccaagaactg
    24481 taatattttt acctgaaagg tccacaggag ttccgattgt cacaacccca ctagcagcag
    24541 tagctggaag tctatttaaa acaggtgcgt tagttccatg atctgatgat atcccattga
    24601 acaccttatc tgcatttcca taaagagtct gtgcactctt actatttatt ccatttgttc
    24661 cggtaatcgt aaaagatttc atatcaagta ggatatcttt atcttgtgaa taatagactg
    24721 tatccacatt attttttaga gcaccaatac caagtagttc ataatttgtg gaactgatag
    24781 ttaaattaat tgaattaaac ccaaccgcac ctacctctcc atttgcaccc ccattggttc
    24841 cacaggtctg ggtggataga tctgttgtta tatatccaaa cccagtagct gctgcgcaac
    24901 ttaaagaaac agatgagtta ttcacaaaag caaaagatgt gaggattgca ttcgttccat
    24961 ttggatcaaa acgaaaagcg gcgttgtttt tatcattggt tcctaaataa acttttccat
    25021 catacttaaa tgcaaaggaa aaagttgttc catcgaaaaa tggatcctca aagaaagaac
    25081 cgcaagcaag tgttgtgcaa gctgtccccg ctcctataaa agttgcacga tcatttggag
    25141 ccgactggat acagtttgtg cctgcagagt ctcgaataca tacagagcca ttattaaaat
    25201 tatcattatc cgtagcgttc gcaacgatta cagtataagc aactcctgcc tgagatccgt
    25261 tatgcgttag agtcactgta aaattactag caccagatcc tacaactgca gatgaaattg
    25321 ttccgtctcc aattgtggga gatattttat agcgaagtcc acattcatag gcagagttac
    25381 attctgcact attggttgta ttcattccag tcaatggagg cttactgaaa gtaatcttta
    25441 ttgtatttgg tgttaatgcg gttgctgcga ctacttttat tttttctaag cctataaatg
    25501 taaggaattt aggcgagccc attgcatttc cagctaaatc agttactgta gaagctaccg
    25561 ttaatcgata ttgtatattg caaaaactct gtcctccaga taagttaatc ttcacagatg
    25621 ttggagaaag aacagatagg gaagcaagtg tggcatctgc acaaccagca tttgtaggat
    25681 cttcaattag agtataattt gcaagactgt ctgcattttg atttgcagtt ccacttccca
    25741 ttctcattgc ttctgaaaat tgaacgatca gagagtttgt ggtaatatca ctggtataaa
    25801 ctaaataagg tgaagtcgaa tcaacgccaa atgatccact aattggagta acactcactt
    25861 tatttgttga agtatttgga gaacttgctg tatcttttag tgtattcact attgttatgt
    25921 aatcaccgac cgcaactgtt ggagaacctg ttccagcaaa cgttagtgta agtatatcgc
    25981 cagctccatt ccaattggct gaagtcaaat ctccccaaac atgactagaa gaaagagata
    26041 atacggatgc aatattagaa atagtaatag atttatcagt aggttctgaa aatgttatta
    26101 ctaaagtatc cccactatca actgtcgcat ttgtatttcc ggtgggggat gctaccgctg
    26161 ttaaaataac gggtggagcc ttatcaacag taactgcagt aagattggat atcgtttgta
    26221 attcaacgcc agtgattgag cgccacctac ccgcactagt cgtaaaagcc attgccttaa
    26281 cagcagttcc agagactgta ttatcatctg taaaaagagt gatagtatta tcatctgcga
    26341 caccagtatc cgtatttgga gtgactattg ttcctgttcc actcgttgct gtatctactt
    26401 ttatagctgc cgttccaccc attgtaaaac gagaggcatc taaatcgtct atcgttgcgt
    26461 cattcatact caagctaaaa acaactttaa tttgatcaat ctttccattt ccatttgtat
    26521 catattcttc aattaaagaa attgttggat tacctgcact gatagaaata gctttagcag
    26581 ttgtatttcc agtattgttt gcagcatcat atagatttga attccctgca actgaatcat
    26641 ctggtgcact cgtatcccca tcaccagcaa taaaatttgc atttgcagta aatattgcat
    26701 tcgaatctga agtggcgcaa gtatctgctc ccatgctaga taatgaggta gcaccaccag
    26761 cgtttgaatt tgcatacgtt agtgttgttg cactcaaatc tccacctgag ccgcacgaag
    26821 gcgctccatt cgttccataa accgcttccg aaaatgtaac ggttaatcct gtttcacctg
    26881 cattaccaga tgcacttaca acgactggct ttgctctatc tgattctgtt actgtagcag
    26941 tgtagagttg agctgctgtg cctgtaggtc cagtcaaggt cggagtagaa gatgttgtta
    27001 aatcaggttt tgccccagtg tcaaatccaa ccccttcagt aaatgccaag taaagaacgg
    27061 catcgtttgc tgtatcacta aatccagagc acacagatgc tacagacgtt ccatgttgta
    27121 attttacatt ggaataacca gcaactagcc aatgagttgt aacacttcca actgaattta
    27181 gaacgtagcc tgggaatgta gagtctgtaa ctaaagtatt gaacgttagt ttataatggt
    27241 caatttttcc gttgttatca caatccatag tttcggcaga agtgacacca atttcatcat
    27301 ctataattgt aaaagtatga gtagtttgag ttccgagtga agcattggta ggagaagaaa
    27361 tagaaaggtc aatagtatcg tttccttcaa agagcaaatc ggaaagcaca gctatcgtaa
    27421 cgttagctgt cgttgagcct ggtgtaaaat ttactgttag tggtgaacct atatttgtat
    27481 aatcagatcc agctgttgca gttcctgttc ctaaatcggt tacaactact gatacagttt
    27541 ttccagaagc agcagaaaga ctcaatgtga ttgttctatt tgtttgtgtt tcatttacag
    27601 aattagatgt agatgctgaa aattgaacgg taggcggcat atcatcgttt acaattgttc
    27661 caataccttg agaggttgcg attgtagcgt tcgcatttac attacttaaa ttcacataaa
    27721 aagtttcatc tgtttcatcc aaagtatcac cagcaacaac aacattaaat gtttttgttg
    27781 tatctcccgg attaaatgta agagtagttg tagtaattgc agtgtaatcg cttcccgaag
    27841 atgcggtgtt gtttgcagtt acataatcaa ccgtaattgt tttaccggaa gctactgaga
    27901 ggctaacagt aaacgttgca ttcaccgtgc tcgcatttcc ttctgtcact gatacattat
    27961 caatactgag agtaggagat gtatcgtcat tagtaatagt tccaactccg gttgctactg
    28021 caatcgttgc actcgtgctt acattggcta aagatacatt gaaagtttcg tttacttcat
    28081 ctgttgaatc tccattgaca gcaacactaa tcgtctttgt agtttctcca ggattaaacg
    28141 ttaaggtagt cgttggaatt gcagtgtaat cagaacctgc actcgcagtt ccatttgatg
    28201 tagaataatc aacagtaact gatcgtcctg atgcagcaga aagagttaca gtaaaatctg
    28261 catttacagt tcctgcattt ccttctgtta cagaaacgtt atttatactt aaacttggaa
    28321 tcggatcatt atcagtaatt gtgtaagtat gtgatgtaat agctcctaag tttgcaacag
    28381 tgggattact gattgaaata ataattgttt cattcaattc atttaatgta tcattgttca
    28441 ctgcgattga aatattttgt gatgttacgc cgctattgaa tgtcaatgtt cctgaggcta
    28501 gagtataatc agtcccacta ccagttgcag ttcctcccgt taccgcataa tcaacagtaa
    28561 ccgttgaacc agagacaata gaaagtgcta ccggaatatt gacagttgtt accgattcag
    28621 ctccacttga ggtggatgca gaaaatccaa cacttggagt atcattatca atgattgtat
    28681 aagtatgttc tgtatttgtt ccaagtgttg caatggtagg atttgccaga ctaattttga
    28741 ttgtttcatt tgattcaaga agaatatcat tattaattgt gatactgata ttctgagaag
    28801 ttacgcctga cgtataagtc aaggttcctg aagctaaagt aaaatctgtc cctcccccgg
    28861 tggcggttcc tcctgttaca gaataatcta ccgtgacagt tgaagcattt gaattagaaa
    28921 gtgtgacagg aattgttact gcagtagttc cttctgatcc attggaagaa gaggatgaaa
    28981 actctactgt agtaggatca ttgtcgttta tcgtataagt atgcgatgtg aatgttccca
    29041 gagtcgcatt ggaaggagca gataatgtga ctatgacagt ttcatcagat tcacctagta
    29101 aatcatttac aacggtcatg tttatatttt gtgtcaatgt gcctgaggta aaggttaatg
    29161 ttccggatgc taaagtaaaa tctgtcccag aaccagtcgc tgttccacct gtaacagcat
    29221 aatttactgt cactgtagaa gcataaactg cagaaagact tacaggtaag cttacagctg
    29281 tcgtagattc agaaccagat gaactgtttg tattgaaggc aacagtaggc ggatcattat
    29341 ctaaaacagt gtaagtatgc acagtatttg catttaggtt tgcgttcgtt ggattggata
    29401 aagttactat taaagtctca tttaattctg tattagtatc gtttatgatc gaaagcggta
    29461 gactcaaagt agtagttcct ggattgaaga caagagttcc tgtatttaga gtaaaatcaa
    29521 caccgcctcc tgttgcagtt ccacctgtta cactgtaatc aacgttagtc gttaatcctg
    29581 aggctgtaga tagagttaca tttaatgtgg gagaagtagc tgattctgca ccactagaac
    29641 ttgtaacaga aaaaccaagt gtcggaactg gatcgttatc attgatcgtg taagtatgaa
    29701 cagtatcaat acctagattc gcatttgttg tgctggcaag agtaataata attgtctcat
    29761 tgttttcata taacgtatca ttatttacag taaaattaat attctgagag gtagcaccgc
    29821 catttgtaaa atttaaaact ccacttgcca atgtaaaatc tgtcccgctt ccagtagcgg
    29881 ttccacctgt tactgaatag ttcacactcg ctgtagctgt catagaaata gacacaggta
    29941 tagagactga cgtcgtagcc tcagaaccat ttgaagtagt agcattaaaa gctattgagg
    30001 gagtatcatc atctacaatt gtgtaagtat gagttgtaat tgagcctaat accgcgccac
    30061 tgcttggatt tgatagagta attatgatag tctcattcgc ttcctgcaat gcatcgttca
    30121 cgattggaaa agaaatgttt tgggttgttg tagcactaga aaaagttaaa cttccattcg
    30181 caagagtata atcaacccct ccccctgttg cggttccact tacagtgtaa tcaacagtcg
    30241 cagtcgatcc taccgaaata gtaacaggaa ttgtaacgct tggtgatgtt tctaaattag
    30301 ttgaactaga ggatccaaat tgaatactag gtgtagtggt agaagtattc cctgctgttg
    30361 gaggagtttc aggtgtagtt gtagtttgac ttccatttgc tggtgagcta ccgcctatcg
    30421 gtagaagagg aatcattcca ctttttttat taccactcaa tagaattgca gaaatggccg
    30481 gccacgcagt gcaattcgta aaaaataaag ttgaaattaa aatattcaaa ataagaaatt
    30541 ttgaattaga agtaaaaatc atttcttctc tccagtattt aaattgactt gtaaaaaatt
    30601 aatttactaa cctagaaaaa atgatttttg gaattgttat ttattttttt aatttttctt
    30661 ggtgggcaac aatggctttg tccaaagatc atgcaaattg cattttacat aaacttttac
    30721 tctcggattt tctgggaatg gatctaatgt aagtttgata tgcggaatct gttttctttc
    30781 aagatcgatc ccagcaatat cctgtttatt ttcatccata actcctatcc tttctatgta
    30841 atgatcttca ttgaattcgc ctgttacaga aatttcaaca ttctcttttt tagattttgg
    30901 tgagattttt atttgaggca gatgattatt tttaatatct tcccattctc ctgaatctat
    30961 ttctgtgtat tctgattttg gagtttttgg ggagaccttt tcttttggtc gaattggttc
    31021 attcgatttt tgttccatct catttgaaca aaaagaaaga ttaaaaagaa ttatgaatgt
    31081 aaaaaagaat tttaaaaaac gaataaataa caacgaacaa actccatctc aaaaaaatac
    31141 tgatagtttt ttagacgaat acattttctg gtgtaagaca gttttttaaa aaaattattt
    31201 ttttagtaaa agttcgactg aatcctctca ctcatatcca attattaaaa ataattacga
    31261 aattaacaga ttgattaagg gttgcttcac gcaatgaact taattctcca atgatttact
    31321 tattaaattc agtcttttta tttgtaactg tcaataaatt atagacaatt tttattgact
    31381 gatttcaacg atttattaaa atgattcccg ctttaaagga tactaaatgg aaacattaac
    31441 aaagattagc tcaacagagg aagaaaatga aacctcaact caaaacaagg acaaaaagga
    31501 caccaagcct aaattctcca caaagagaga tccatcggtg agaatgtcct acattgttcc
    31561 cgaagaaatt tcctctgaag atttagattc ttaaattgac tatagagtga atcttttctg
    31621 aacaattcac tcgaggcaac agacatactc acaaagaccg aaaagatctt aaagctaagc
    31681 tcttccaatc aacatcgaaa accgtataca atcaatatca ctatttttta gcgattacaa
    31741 atgactctaa agcatctctc gctttttcat ttgcattttt ccctgaggca atacttgtat
    31801 ttaattctaa atacttacca aggcctctaa cttctaacgt ttcctgtttt gttttttctt
    31861 gaggacaatt accattcggc gaatccttta aaacaaaagt tctatagatt acaccaattg
    31921 gttttttatt taacaatcgc cattctaatt ttctagtctc tgcgctcccg attggttgtg
    31981 agggcatatc gatggattct tttttatttt ttgactcaat tcgcaaattt tccatgcaag
    32041 cggaatagga gataatgact gtataccccg aaggtccatt gcaaataagc ggagcatctt
    32101 gcccttcttg tatatcttta gaatcatact tacaatcttt tgaaatgtta gtatattcac
    32161 ttgtaaaact atcctttgca aataaagatg agtaaaaaac tattaatatt aaaaatactt
    32221 tcattgttca ttccttatac taatcaacca tttcaacttc ttctgaattc cgatttataa
    32281 tttcaccgat aattttagcg gactctccta aagcggctaa ttcttgtaaa acgctatttc
    32341 ctctatcttt tggaattatc agtataaagc ctacccccat attaaaagta gaatacatgt
    32401 ctttctcatc taaagaatga tcttttttta tcttattaaa tacataactt tctggtaaat
    32461 ctttcttaat aattctagca gccaactctt taccgaaaat ccttggaata ttttcataaa
    32521 atccaccacc tgttatatga atcattcctt tgatttcttc cttctgaagt aattttaaaa
    32581 ttggatctac ataaatgcga gttggtttca ttagataatc tgatataaag gctaatgctt
    32641 gtttgtcgct aggtaatgct ccattttcta agtataactt tcgaatcaag gaataaccat
    32701 tgctatgagg accacttgat tctataccaa caatcaaatc tccactttga atttttgatc
    32761 catcgatcag ttcagatttt tctacagcac caacagcaaa accagcaaga tcatattcat
    32821 ctggcttcat cgttccagga tgctctgcag tctctccacc taccaaggca gctccacaaa
    32881 gtttacatcc tgttactatc ccagatacaa tcttctccat cttttgaata tctaacttcc
    32941 cacaggaaat ataatctaga aaaaataaag gtctagctcc tgatacaagc aaatcattta
    33001 cactcattgc aactaaatct attccgatag tatcatgttt gtctagcaat ctagctaatt
    33061 cgagttttgt tccgactcca tctgtgcaag aaagtagtat tggctctttg taatttttaa
    33121 gaaaagagac gtcatacgcc gcagcaaagc cgcctagacc gcctaaaact cttggattat
    33181 gtgttgagtg cacatttttt tttattgaat ttacgaaatc tctaccagct tcagtatcaa
    33241 ctccggcatc tttataggtt attggattat ttgacatagt ttctctctgt tgttgtttca
    33301 ttgtttaaat aattataagc gagttcaaaa tcggcttctg ttccaccatt taaaaataaa
    33361 tctaatttac tttctccctc atttggtagt ggggtaataa tacgtcttac atgatttgca
    33421 gttccacgat tcccatcata caatttaata tttggattcc attcatagaa taatgactta
    33481 atcaaaacat aatgagtgca acccaagaca atattattaa ttttatcttc ttctaaatct
    33541 ttaaatatag gctgtaaata ttttcgagca ccgtccatat caccactatc gattatagtg
    33601 gctagaccgt cacacggtat aggttttaaa attgtatggg cttctagtgc ctcacctaat
    33661 ctcataaatt tttcttcttt gagagtaagt ggagttgcaa gaacagcaat cttttgatca
    33721 gggttctcta aaactgcagg ctttatcgct ggctccatcc caaagattgg aatatgatat
    33781 ttttttctca gcttatgaat tgcaaccgat gttgcagtat tacaagcgac aacaatcgca
    33841 ctcacttcct tggagataaa aaatctacag atctcatcta tgaatgtata cacctgatca
    33901 ggagatttat ttccgtaggg agaattgagc agatctccat aataaataaa atctgcctct
    33961 ggtattacat tactcaattc ggataatact gataatcctc cgaacccaga atcagttacc
    34021 ccaattttta cacgagtatc actcatttga atcaattttt tccaatcgtt ctatctttgc
    34081 aatatcttta tagggtatat cgatatagat agtatttgct ccaatcgata gagaattcaa
    34141 attttgaatt gagtctagac ttaagaaaag tctcaacttt tcctttttgc gtatgacttc
    34201 cattgagcga tcaaactttt ctctcaattc ttcttctggt ccataactca tatgtccagt
    34261 tcgaataaca ccatttttca agataacttt aattgaaatt aaatcaggac atagcttaca
    34321 ttctgccaat aaagcagaat aagttatcat tacaaaaaaa gcataaataa aatatttgcg
    34381 gctaatcata attttccctt aagagtcaat atccttttaa ttcaagatat cctttcccat
    34441 tgattttttg ttttttatat atacctgttg caactattgc tccttcccaa tatgtattgc
    34501 ctgtcgtctt aaaaccaata aattcttgat tattaaatct tgtctcaacc actaaattta
    34561 aatcttctcc tattactttc catttcatag gataattctt gttagtaata ggactcttcc
    34621 aaatttcatt taaagattct aatttcattt ttccatccgg tttaaatgtt tcaatcctgc
    34681 cattagaatc tctaaatgtg ccaaatgtct ccggttctgt gtttttattc ttacgaaaat
    34741 taaaaagcat caaatcagat ccatcactta gattcagaca gagccaatcc catgaatttt
    34801 cttctgaaac aatagtagat tttaattcag atgaatcttt ttcaggagca ctccactcat
    34861 gatccatcca tgatgttcca gactcaattt caaatctctg agattcaatt tgaatataac
    34921 cctctgttat aagtcttgga attgaataat aataggaata gatatttgaa tccaaattac
    34981 ttttttggga atatccctct tttccatgca acaaaatatc gtttcgagtt gatgtgagca
    35041 gtaaatctag tgagattaga tcctttcttg gctttgcaga aattcgaaac tttgcttcac
    35101 ctagtatttc aattttataa tctccatttt ttatcacata actcgtatat gccgctaaat
    35161 ttccaattct tctttctaga ctttgtgatg tgtaatggcg ttttgttgat agatttgaaa
    35221 tagcaaaatg aacaggaaat atctccttct ttcctcgcat atttgcgcga aaaaagctga
    35281 gttcatatcc aaaatcttgt cctgttttag cttttaaatt acctacaaag taacaccatt
    35341 ctatttgaaa gtctttatgg aaactatgat cctttggaaa ttcaaaagca tctagactta
    35401 aatacattaa gttgataaga ataaacaaaa atattttcaa tgaattcact ctcaattaaa
    35461 cacaggaaga taaaagtatg aatttaattt catgcaaagt agtatataga tttctttttt
    35521 atcattttta gaataatagt atcgaaatgt atattttcct ctcctattta tttacaaaac
    35581 agcaaatgct gaatcaggat ttttaagttt tactaaactt ccaattcatt ttgacagata
    35641 ttaaaaatga gtgaagtatt taggaaattt tgaatagttc catccctaag tcaatccaat
    35701 ttaaaggagc cgttacccat ccaattttga tgagtaacag ataacatttt gatttcaaat
    35761 tcatccaaac caattttagt taccggcgga acaggctaca ttgcatcttg ggtagtaaaa
    35821 tatctattag aagatggtca ttcagtccat acaacagttc gcgataaatc aaaaaaagac
    35881 aaatatgcac atttagaaaa aattgcacaa gagtccaaag gatcactcag tatttttgaa
    35941 gctgatctta tgaaaaaaga ttccttcttt gaagcaatgc agggatgcga attagttata
    36001 catatggcat ctccattttt tgttgttgga ataaaagatg caatgaaaga tctaatagag
    36061 cctgcaaaag atggaacagc taacgtttta aattcagtga atcaaacagc atcagttaaa
    36121 agagttgttc taacatccag ttgcgctgct atctatggag ataattgtga tattgagcaa
    36181 actaagaata aaattttcac agaggaatat tggaacagaa caagctcaat caatcatagc
    36241 ccctattcat attctaaaac tgttgcagaa gaagaggctt ggaaaattgc aaaaacgcaa
    36301 tctagatggg atttactcac tgttaatcca gcgtttgttt taggtccctc tctcaccaaa
    36361 agaacagatt caactagtat caacatgatg atccaattag gaaatggaat gtttgcgtta
    36421 ggcgttccag aactttggtt tggaattgta gatgtcagag atgttgcaaa agctcatata
    36481 ctggcagcat ttactccttc agcaaaagga agacatatcg catgctcaga atgccttcca
    36541 atggttgata tagcttctgc tcttcgtgaa aaatttggag atcattaccc atttccgaag
    36601 tggaatgttc cctatttcat ggtttggtta tttggtccac ttcttggatt tagccatagt
    36661 tatgttgaaa aaaacattgg cattccgatt aaatgcgata actcctacac tcaaaaagat
    36721 ttaggaattc aatttagaag tgccaaagaa acattttggg atcattttga gcaacttcta
    36781 aatgatggaa ttattcaaag aaaaggataa ttaacccaaa taaaacttca ctgttttttc
    36841 aatcccagtc tcgaatgttt cttttgggaa ccagtttaat tctttctgta ttcgagtagc
    36901 atcaatagca taacgtctat cgtgccctgg acgatcggtt acataattaa ttaatttttc
    36961 atattcgcta actggcttgt tagttaattt acctaatact tcacatattt ttttgacaat
    37021 atttatattt gtccattcat tattgccacc aacgttatac gaacgaccac tttccccttt
    37081 ttgtaaaata gtccatatag catcacaatg atcctctaca tataaccaat cgcgaacatt
    37141 ttttccatct ccatagacag gaagtttacg tccgttgatc gcattagtaa tcataagtgg
    37201 aattaatttt tctttgtttt gatatggtcc atagttatta gaacaatttg aaatagtaat
    37261 aggcatacca aacgttctat gaaaagcatt cacgaaatga tcggatgctg ccttggatgc
    37321 agaatatgga gaagatggat cataaggggt agtctcagta aaaaaacctg tttcacctaa
    37381 cgatccataa acctcatctg ttgaaatatg atgaaaatga acaccttctt tatacttacc
    37441 atccttttgc caataggttc gtgcagctgt tagtagctcg aaagttccca tcacattggt
    37501 ttcggcaaat attctaggtc ccgcaatgga attgtctaca tggctttccg ctgcaaaatg
    37561 aacaactgta tcaatttcat tttttgtaaa aacgaaattt aaataatcct tatcacggat
    37621 atcgcccttc ataaattcat agtttaaatt tttttcaatg cctgtcaaat ttcccaaatc
    37681 accagcatag gtgagtgaat ctaaattgag cacatgtcct ttaaaaccaa tttttgcgag
    37741 atagtgaatg aagtttgcac cgataaaacc ggctccacct gtaactaata atttctttaa
    37801 tgatctattc atatacaatt cctaaattat ttcccttcta actaagttga atttttacct
    37861 atttttgaaa atacatttct atctcaaaat ttagattttc tcgagcctct ttttcatatt
    37921 tactgtagat ttcatctgta aaataaatcc gatttctttc tagaaatttt ttcaatattt
    37981 tagatcgttc tgttttataa atttcacgct ctacccaatc atactctttt cgaatactat
    38041 ccatatattc tatatatcga tttcgctctc ttccaagtat cagcaaatca gaatccaaga
    38101 atattttatt atcaaagtca tcctgtaaaa attgatgagt ctttgttcct aaaattaatt
    38161 taccacactt ttctatttgt tcagaagaaa gatactccga aaaatctttt ttaagatatt
    38221 cataactctt ttcttcgtta tcattcgcat gagtatcata aaatatatca tgatataaac
    38281 aagctatata gagaagtgaa taatctacta tgttttcttt atagagaaat attgtttcta
    38341 acatttcgcg tatatgcctg agattatgat aacttctatg tgactccgaa taacaatcag
    38401 ttatccgacc aaatatactc aatgcttttg cttctggtat acttaaagac tcaaagacct
    38461 ttattgcatc tgtttttaat tgaatcaaat tattctacct tctactattc ttttgaaatc
    38521 ttttttccaa aatataataa attaatggag acacaagcat tgttaatacc aaagaagaga
    38581 aaagtcctcc aacgataaca gttgccaaag gtctttgcac atcagaacca atcccagtat
    38641 tcaaagcggc aggcataagt ccaataatag caatcatcat aaccaaaaat cttggtctaa
    38701 actgtatgac agagccttga attaccattt ctttcagcgg caaatcagta ttatgcttca
    38761 tatgattcaa aaagctcaaa agtaatacac cagacattac cgatattcca aataaggata
    38821 caaatccaac acctgccgat atactaaaat gcattccgcg aatatacaaa ccaatcactc
    38881 caccaataac agcaaatgga atatttgcag tgacaatcgc tgcatgaata taattgtttt
    38941 tgaagaaaac caagagagtt aaataaatta gcaataatgt caagggaact atatacgtta
    39001 gtctctcttg agatcgtttc aagttttcaa attgaccacc aagatgaaat tttacattct
    39061 ctggaaaggg aatattcttc tcaatcttgt ttcttatttc atctgcgaaa cttccctgat
    39121 ccctctcccg tatattggtt tttactgtca tctgtctacg cccgtcttct cgacaaataa
    39181 ttgtttgccc ctcttccaaa ccaacagaag cgacacgatt taaaggtatc ctttgaccgg
    39241 ttctactcgg aattaaaatc ttttccaagg attgaggcgt atttctactt tccacagtaa
    39301 aacgtaaaac gatatcaaat tttcgttcat cttcatataa atgacttacc ggtaacccag
    39361 caatagcagt tttcaaaatt ccatttacat cttcaacgtt aattccatat ctagcagcag
    39421 cttgtttatc tattcgtata acgacttgcg attgtttatt ttcctgctct atcgatgatt
    39481 ctgatgctcc atctgtcgat cgtgtcaatt ctaaaatatt cactgcaata tccctcaata
    39541 catccaaatc cctgccagta ataaaaatag ccaagtcagc agaggatcct gtcgcatttt
    39601 ccgtagtagt atctatgatt ggctgagtga ttgtaaaagc agcacctgga agcaaaagtt
    39661 ccagtgtttt tttaaactct gtaatcaact ccttcttagt tatttttgat tcccacttgg
    39721 agtaaggctg ttttagttga actaaaactt ctaatcgatt ctgaccatat ggatctgtcc
    39781 catcatcatt tctaccaagt tgagttaaaa ctgctcttac ttcatcaaat ttccccaact
    39841 cttttcgcac aacgtctgga tattgtctag ccgcttctag agaaattcca ctcggaagga
    39901 atattttaac ccacaccgat ccttcatcta gttcaggaag aaactctgtt ccaagtccag
    39961 gactaatact aaaaataaaa aacacaaatc caaaagatgc acctagaact ggctttggaa
    40021 tcgaaaaagc ttttttcagg atttcgccgt atccattttg aatacgaatc caaattggat
    40081 tttcatgtgc aattttagaa ttttgaaata aaaatgtcga aagaacagga acaaccgtta
    40141 gtgcatatac cattgaacca agaatcgcaa aggaaagagt ccatgccata ggggcaaaca
    40201 ttctaccttc tactctttgc aaagtcaata taggtagaag agctaaaata ataattccaa
    40261 ctgcaaataa tatttgtgtc tgaacttcag cagaagaacg aataacaagg tgcgacaaat
    40321 tcgtattatc ctcttccttc ttttctgata aatgtctaaa aataccttca accatcacaa
    40381 tcgctgcatc tactataatt ccaaaatcaa tggctcctaa tgaaagtaaa ttagcgggga
    40441 tattagcaat atccatacag acaaaagcaa atagaaggga aaaaggaatc gctagagaag
    40501 aaatcacagc tgctttccaa ctcccaagta aaatggttaa aacaataaat acaactactg
    40561 ttccctcaat taaggtttga gctacagttc taattgttag tgcaactaat tctgttctat
    40621 catacaacgt ttcaattcga acaccatcgg gaagtagtcc attatttaat tcatttacct
    40681 ttgccaatac attctgcatg gtagctgtcg gattttcaaa ttttctaagt aaaacaattc
    40741 cctcgatacc agcatcatct tctttctttc tcatcttatc caaataacca agaacaccag
    40801 ttggaggata tacaccgatc tcaacactag caatatcttt aattaagata ggaactccat
    40861 gcctgttatc tacaacgata tttccaatat cctccaactt agtaattcta ccaattccac
    40921 gaatattcat ctgcgaagaa ccaaggatca tataacttcc acccgtattc tcattattcg
    40981 aatgaatcgc atctgctata gattgaatac taagtccata tttttctagt tgaattggat
    41041 taataataac ttggtattgt tttactaatc ctccaaaatt agcaatatca acaactcctt
    41101 cagcttgtaa taattttgga ataatgacat agtcttgcaa ctctcgaagt tgagtcaatg
    41161 gaacgttatc cggtgcctct atcacgtatc tatatatttc accgatagat ggagtcattg
    41221 gaccaagaac aggactcgca atcggaggaa ggtctgcatc tctcaacttc tcttctacca
    41281 actgtcttgc taagtaaata tttgtattgt cttgaaacgt caatcgaaca atagacaatc
    41341 caaagattgt tttagatctt tttgaaataa ctccgggaac agtattaaga gctctctcta
    41401 tcggaacagt aacctgtagt tcgacctctt ctgcaggaag tccatttact tgggttatta
    41461 cacttacttc aatatcacta atatctggat aggcaacaat ttctaattgt gtccaagaat
    41521 agattcccac aagacaaaaa ataaaaataa ttgcgagagt taagaatctc ctatgaacta
    41581 caaattcggt gagccgatta aatcccaaaa gacaacccct ttaataaaaa gacgttagta
    41641 gtaactattt cttctcctgc cgtaatacca tgagcaattt caataaattc ctcggtatct
    41701 gtggatattg taacttccct tctctgaaag gtagtggggg aaattttaat aaatacataa
    41761 tagcgtgcat ttgaactcac aagtgatttt ttaggaatca taagaccgct ctccggcatt
    41821 ttaaactgaa ccttaccata cattcctggt ctgatcttat cttttgtatc atgcatgatg
    41881 attcgaatcc tagcttttct tgtatctgaa tttaaaactt ctgcaattgc atctattttc
    41941 cctgaaaaag tttctgttgg aaaactagga aaacttaaac tacacgttag tccctctatc
    42001 aatatattca attcagattc aggaacatcc gatattagcc agacagatcc tctggatgct
    42061 ctttgtaagt cctttggatt aaatccctct cttctgagtt tagcttcatt ctccgcaaga
    42121 gaggattgtc tattataaag ttcggctgct gcgtcattta actccttgcc tgttgcagct
    42181 ccatgatcag ctaggtcttt aaaccggtta taatttgttt gagcaatatg gattaaagat
    42241 agattttgta agtaagcagc ataagcacta gtcaagtcag gagaagcaaa gaggataatg
    42301 gatggtaacc ccggagcttc tgccttttta attctaccaa ttactgtagc aggtgcattt
    42361 atgtttaaat cataagaaag aaatacaact ttttgagttt ggaataaatt gatttgagga
    42421 gcgtcaggcg gaaattctat gtgttttcca ccctcatgaa tcattgcacc cgaaccactg
    42481 agaggtaaag gtttcttctc ctcatttcta tctttcttac acctacaact ctctaaagag
    42541 aataaaagaa ttaaaaaaag aataaatatc ttggttgaaa atctcataga tttcaattta
    42601 aaaattttgg atttttccag tgacatattg cacctgtata tagctttttc taagattaaa
    42661 tatagattcg taaaaagaat tttgtgcatc aaaccagctt ctttgcgcat ccttatagtc
    42721 aatcactgta gtcccacctt ttaaataact atagcggact gtgtttaata ctttttctgt
    42781 tgtttctaaa atctttttat atctctcata atttgcttta gaaagattat actcacctat
    42841 tgcattttga acatctgtgt ctaactgttt ttctagagcc tcttgtaaat tcatgacttg
    42901 tttcaactgt gaatgtgatt tttgaatctc accttgattg cgattgaaaa caggtagtgg
    42961 aactgtcacg taagtgccta aataaggaga gccattttgt ggattgtaaa taacgcccac
    43021 ttccggttgc ggataaacgt aggcttcttg taaattaata tttactcgat ggacttcttg
    43081 atgaactttg tttgccacaa tatcggctct attacctaac gcatactgaa ttaaactttc
    43141 tttagattca tccagctttt cgtaagatat tttattatct tccacattaa tcaatatctc
    43201 tgtattcaca ccgcttagaa attgtaaatt tctagactcc tttttaagag ccatttctgc
    43261 agaagctaaa tttgcttcat attgctctac aatattttga gttcttaaat attcggattg
    43321 agtaatcact tgatttctaa gccgtatctc atttatcttt aaaaggtcat ccgagtattc
    43381 ctttgtgcgt ttaataatgt ttaaccgctg attggcaaac caaacatcta tccatttatt
    43441 tgcaacgata taaagtaggt ttctttcaaa ctcaactaag ttcttttggc taaattgcaa
    43501 attttttaaa gcaagctcga ttgaatattt tctttgtcct gctactggaa ttttttgcgt
    43561 caattgaatc cagtcctgtc gatttgcatc attccaagca cctcccctac ctccactagc
    43621 gggtaaatag ggtaatgcgc cagatgcagg actttgacga atcaactctt gattatttaa
    43681 caatggattc ggaagaagtt tagaagtagt aacatccgca tttgcaattg caatgttctg
    43741 ctgctctgct atataaaagg gattattttt tcgagcaagt tctagagagt ctgttaaaga
    43801 aagtggcttt gataaatcaa ttgagtaaat tgatacagtt gataaaaaac aaaataacgt
    43861 taaaataaat gtcatataaa taacaaaact acaacattat ttcttattcg atttaaaata
    43921 caagtagaat taagatcgat tcacatcttc ttcaaattta gctttattta tattaccaaa
    43981 ttgttttgtt tctttcactc ttaaatgtct gtaaaagtga tcatactcat ccatcattcg
    44041 atcaaatggc aaatcaataa aacttccatg atctactaga tactccatat gtttatttcc
    44101 agatttatct aaaggatgaa aaatagaatc atgctcacca tcgaactcta acggtaaaat
    44161 atttgcacgt tcacaaaggc ttagattaaa tgctggagtt gcttttaccc attgtttgtt
    44221 taaaaaaata tctgtatagc catgaaatac aaatatatcc gattgcatca agtcttgcaa
    44281 tcttttagag tttaaatgat tctttacgtt tgcaaaacct actttggcag gaatcccagc
    44341 ggagcgtaag caagcggcta acgttacagc tttttctaca cagaaaccat atttacgttt
    44401 aatcacagca ctagccctta aagcttcagg agttaaaata atgttatatg gatcatagcg
    44461 aatctcatct ctaacagcta aataaatttt cttggctttc tctacatcat ttaactcttc
    44521 tagatttagg ttttgtataa attcttttac ttctgtagaa tctgcatcta aataaaatgt
    44581 agattttaat tcttctttaa tatccatact ggaaatataa tttctgattc tatttaaagc
    44641 aagaaagaaa tgagattaaa atgagttaac cctttagata ctctgcgaat ttttaaaccc
    44701 caatcatagc ctttttcacg atcaagattc atgactaatt gaaattttgg aatctgcgga
    44761 aagtaattgt taaacgtatt aaaatgcgac gaaggatttt cttctcctaa cttaacctca
    44821 atcattaaag aaggcttatc atctaaagta ataaggaaat ctatttcttc tccttcttta
    44881 gttcgtagat aatggagtcc tgtttttatc ctctcaaagt cttctaatcg atgaagttct
    44941 ttatgaagag aacaagccac taaattttct agtcgagctc cctcgccttc cgttatggct
    45001 atattataaa aataaaactt tggttctttc aaaatagaac gagaaatatt tttgttataa
    45061 ggagtaacaa gaaatacaat atatagattt tccaaaattt caaaccaatg ttttattgta
    45121 gctggacttt tttgcaaatc tcttgcgagt gacgcatagg aaacaggaga accaatccta
    45181 cgacgaagta attctatcat tgtttcaatg gaggtaacat ctcgtaaaga ttcaagatca
    45241 atcatatctt gtctcagaat tacatccaaa tgggaacgat accatttttt ataaaaggat
    45301 tccttgcctt ttgtaaatgg tcaggaaagc ttccaatcgc gagtaacgtc tttaaaattt
    45361 catctgaact tccctggttt gcgagttctt ttaaatcaaa aggatgaagt cgatactgaa
    45421 aaaatcttcc cactagcgaa tccccgaccc tctagcaaat tcaagcctag cactgccagg
    45481 aatcaaaata gcttttatct cgatgattca tagcgactat tttcaagttt ataactaatt
    45541 agttaagact aaaacgaagt gctattttaa aaagttttgg cgcatcaaaa ggacaacggc
    45601 cactgaggca ttcctatttt tattccattg aaagggaata tccagcttta acgtgaattc
    45661 gatattcgat ataaggatgt gacaaattat ttagaccgac attcccgaaa ttccacccgc
    45721 tatcgccgct accgccaagc gatatcggga atctataatt ctagataccc ctcggcggta
    45781 atggatgcga atagcatcgg gtggaattcg ggggtgacca atagaaacat gtcttacacc
    45841 aaactcaggc tactgtagag agaaaaatcc tcttatcagt tactcaacct atctgtgtga
    45901 atccgtggca tcggtgctag tcttttaaaa aaacttggga atgatagaat tttaaacaat
    45961 accaatctaa ggtgtgagtt attattctga ttttaaaaaa agctagtttt taaaaactgg
    46021 agacatgcca tcatcttggg gaaaaatttt tagagtaagc acatttggag aatcacatgg
    46081 tgattcagta ggggttgtta ttgacggagt tccaggagga attccattta agttagaaga
    46141 gattcaaaaa gacctaacac gacgtagacc aggtcaaaac ttattgacca ctcctcgtaa
    46201 ggaagacgat gaggtgagag ttgtatctgg aatattcgaa ggaaaaacaa ttggtagccc
    46261 aattaccttg attgtagaca acaaaaatca tctttctaag gattatgaaa atttaagaca
    46321 tatctataga ccctctcatg cagattacac ctatcatgct aaatatggtt ttagatccca
    46381 cgtaggcggt gcgcgtgcct ctgtgcgaga aacgataggc agagttgcag cgggtgcatt
    46441 tgctcgttgt atattagaag aagaacttgg aattcaaacc attgcttggg
//
LOCUS       DAOQOS010000036        45618 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_314_length_45618_cov_6.531308, whole genome shotgun sequence.
ACCESSION   DAOQOS010000036 DAOQOS010000000
VERSION     DAOQOS010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 45618)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 45618)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45618
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_314_length_45618_cov_6.531308"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..270)
                     /locus_tag="PLF66_09815"
     CDS             complement(<1..270)
                     /locus_tag="PLF66_09815"
                     /inference="COORDINATES: protein motif:HMM:NF025021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HMZ64379.1"
                     /translation="MKKLLIITFFILNISLYANNWNDALYEASENGDLNKVKLAIEKG
                     AKVDYQSMDKGGTTPLMIASEKGYIDIIKFLLGKKAKVNLKDNEEY"
     gene            complement(267..2432)
                     /locus_tag="PLF66_09820"
     CDS             complement(267..2432)
                     /locus_tag="PLF66_09820"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HMZ64380.1"
                     /translation="MSESDFLFNESIVPIAYLDGNGNYIKVNSAYASLFCYCKEELIN
                     QNFTIHIKNPKEKEISLNEYYSFFTNTANDSITITLENKNKKSLTVKIIRKLLRLDTN
                     IQSAAFFIQNVIDEKISSEEILNAVFNAIGGVIYHVRYETNQKRKMIFISEGAEAILG
                     VPNHKIMEDISILENHIHPEDLLSISTSLDLFKKNNNFRTIQYRVKNKTGGWKWLEER
                     SILLESNSKDHSAYGMIMDITDQRTKNKRLEDLRYSLDQSSIVSVTDINGVIIDCNDN
                     FSKISGYTKEEIIGNDHRLINSGYHSKQFFKNLWDKISSGKVWEGEINNTRKDGSEYW
                     VYTYIIPLLDENGNPFRYISIRNDITPRKLAERQLAASEEKYRMLYQNAPIGIVIVDK
                     NGTILDTNDYLLNLMGYKRNEFVGKKYSDFLQKDFLEYKQDADHRLFDGQINRFYIEQ
                     KCITYDGHPIWLGCYSNAVKDNYGNVIYRLDFAIDIENRKKSEEELLKRDQSKNAILN
                     IVAHDLRSPLSGITNLARLLLKQESDETKYKYLELIENAGNHSMSIIQDLLEMVKLEQ
                     DPQMIDMELTNLNTFLQDCIRMHEFQCKEKSISIKYHSSIEKVFHNINQDKMMRVISN
                     LISNAIKFSYDNGIIEIHLYREKQITVISIKDHGIGIPKETQSFLFEKFSRARRIGTK
                     GEKTIGLGLSIAKEIIDKHQGKIRIESEENKGTCVYIEL"
     gene            complement(2732..3124)
                     /locus_tag="PLF66_09825"
     CDS             complement(2732..3124)
                     /locus_tag="PLF66_09825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64381.1"
                     /translation="MKILFFLLAICLVTECATIFEETEETPENPYRAAKLRQLKSKVE
                     CIDCDPTEEELAEYHRLKEPLEVVTRRISYSNSWQNYSRTYYLYFPNNIKNPWYTPGS
                     LGTVNGGRAPGAYYPYGSLQPGYYGRIR"
     gene            3232..3933
                     /locus_tag="PLF66_09830"
     CDS             3232..3933
                     /locus_tag="PLF66_09830"
                     /inference="COORDINATES: protein motif:HMM:NF014619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase family protein"
                     /protein_id="HMZ64382.1"
                     /translation="MIEKKIQTKNNEEVRIYVLGKKETEQLEGVPKENLAVEIQNLIH
                     SRFSFPKNKIFLLDQTHSDLFYNTNQIRPNQEGDALYSCNLEEILVVKTADCMPLFFW
                     SETERLIGIIHSGWKGTDLGISEKLFKFLKSKGHKIESIQGFLGPCARKKNYEVGHDL
                     FERFSLYAPSAIEPKENGKYSLGIDLVLKKRLQENLIDILLEDSEICTIENTEYHSHR
                     RKDTGRNLNIIYRRG"
     regulatory      3959..4099
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00379"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-AMP (ydaO/yuaA leader) riboswitch senses
                     the signaling molecule cyclic di-AMP; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="cyclic di-AMP"
                     /db_xref="RFAM:RF00379"
     gene            4257..4922
                     /locus_tag="PLF66_09835"
     CDS             4257..4922
                     /locus_tag="PLF66_09835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64383.1"
                     /translation="MKVLVAFTKPDTGRAISQLASKLEGIREIQVVTVWNQKEISIPS
                     ELNRELDEIASMLVNSSIPFKIEMIYGENVPLEIQNAANRMNADAVLLGAANTVFSSD
                     LFGGRVAEVMKGFSRPVYILANKNLSYPFDPVLLTCNDAPYNFLEKNEPLSGLSLDSI
                     PLLANQVVPQKSSYWDLKGEFDLDKRWLKPYNLIVTDYKTYSLYDDFFIKESDQSCLV
                     YCN"
     gene            complement(4924..6351)
                     /locus_tag="PLF66_09840"
     CDS             complement(4924..6351)
                     /locus_tag="PLF66_09840"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HMZ64384.1"
                     /translation="MESLDYCSVADIQYICLSDLHFGAYNSLLTFTDSEGRLDPQKSS
                     DVLVQFLNLLEEIITKTNKKRKAKLILNGDIFELALANTNESVMTFDCFLDLAFTKSR
                     KEVFSDKLIYIPGNHDHHLWETARERQYLEYMIKQNPGEFIEPPWHHTKMIKPTPLKS
                     RFISTLMQRHKHLREGRVMTVYPNLTLVDRDTEKYITISHGHFIEKIYFLMSNLQSIL
                     LNSKNSPESIDQIERENFAWIDFFWSMLGRSGNVGTSIGIMYDMLQDEKSLNELSNNL
                     VSYLMKRSDFPASLQTMLKPITSYILTKIVSQIAINERGLTADILGKDALEGLYAYIS
                     GPLKNQYLEENKNQIPQYLEFIFGHTHKPFCERRTGFKNYGYSDSIGIINTGGWVIDT
                     IEPQEQVGAAIALIDEDKNTVLFRLFNETDLKIKMESINPENPLFLKLKDKLDLDSKV
                     FSEFTQSVERGIQNKRLVIKKRIKN"
     gene            6586..7524
                     /locus_tag="PLF66_09845"
     CDS             6586..7524
                     /locus_tag="PLF66_09845"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HMZ64385.1"
                     /translation="MRKIKTVFLILAQIILLQDCSTYVPKLTDSGFSDSRELSESQLL
                     KPTSKKLVELISFHTGEIEVDTSILLSLKKEKKLKNKKFYVPVLSHLVRHSEKGDILI
                     DTGMDESFSKSGHGNFGCLAKLVSFARQKPGMDIGAQLKSKNANLSAVYFTHAHLDHT
                     SGVPALPKNIPYYAGKGTIDDSFADIFGVCSNHLDGIDSIKEIDFNKAAIVKELGKVL
                     DIFGDGTLWAIHTPGHSAGHMSFLVNTKDGSYLITGDASHTGYGFKNKIPPGKNVNLE
                     QTEETLNKLIQFVNRHPEIKLIFGHDIQNTFQPEVD"
     gene            7525..8085
                     /locus_tag="PLF66_09850"
     CDS             7525..8085
                     /locus_tag="PLF66_09850"
                     /inference="COORDINATES: protein motif:HMM:NF024701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HMZ64386.1"
                     /translation="MPRQGLNRNSVIEAAIDLAEREGFNSLSLGELARQLDIRPPSLY
                     KHIASLEDLQDEIGIRGIEALIEALSKADTIINPQDSIHAICTIYRNFALKNKALYEA
                     IQPAMNRRSAKFQKSAQKLLETILVYVMKLKINKKDQIHVLRSLRSLIHGFIELERQG
                     GFGMKEDLDKSFQYLIQIFIEGVVRV"
     gene            8125..9216
                     /locus_tag="PLF66_09855"
     CDS             8125..9216
                     /locus_tag="PLF66_09855"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HMZ64387.1"
                     /translation="MLPLFAESIKIGILSKYNLSEIDLDISDNTRMFYDGRDLKLKKG
                     LIKVYSQKDKIHFGKSIIAKSILINASENIKLSFSKENHVLERNYKGDLEFISLDGRL
                     QLVLSIPLEEYVSSAVQSELGELLQDGRYNSEAHKKELIAAQEIVIRTFIKKEKDRHE
                     KEPYDFCDLTHCLHFEGTQKTKSIYPGVILKGKDSISGYFHSTCGGILSGPEVYWAKH
                     EISTHYKRGKDGVSAYCSDSPHSEWETILTERELENILNDRKISNIFVVEKQNRVSHL
                     KYKSEQFIKTIPISTFISRTGKLYGWNKIKSNLFKIQKLRERYLFVGKGLGHGIGLCQ
                     WGSAKLAANGKTYREILEFYFPDTELSKQ"
     gene            9213..9935
                     /locus_tag="PLF66_09860"
     CDS             9213..9935
                     /locus_tag="PLF66_09860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64388.1"
                     /translation="MKNKIKIIVLLFLTVQVCLAKPTLFKLDQVHYFYHGNSFSENRI
                     EYFHEIFKQTYEEVFAKFKERKVYPIRVEIFTNTKEFQEHTKLAPIYAGLYTPKEGFI
                     FLLTPKTMNRDRLIQIISHESCHALLDLTNEEIDRVLEEGFCSNEFPSEEFLYIWNRT
                     LLNLELEKFLETGNWILKNSSQAKKRDVYHAATLFIRSIRKTKNTEEVLKLLRANSLR
                     NLNKYWHEFTYNQRLEQQNNKH"
     gene            10087..11055
                     /locus_tag="PLF66_09865"
     CDS             10087..11055
                     /locus_tag="PLF66_09865"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - NADPH:quinone reductase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase family protein"
                     /protein_id="HMZ64389.1"
                     /translation="MKAYVAENWCEPEELVFKDVEDPTPKEGEVVLDIKASGMNFPDM
                     LLIQGKYQMRPKRPFSPGIEVAGIVSAVGPGVTKWKIGDKAMGLSWLGGYAEKVAVAE
                     RNVYPMPDGMSFEEAAGFIVTYQSSYFAIVVRANLKPTETMLVHAGAGGIGSSAIQIG
                     KAIGAKVYATAGSEEKLDVIRNLGADLALNYNDATWSKKIRKEYGGVDVVIDPVGGDA
                     FDKSILCTNFEGRIVVVGFTSGRIPTLAVNMLLLNNISVMGVYWNLYQNDRPQEIEKC
                     VKQLNEWYTQGKVKPLVYKTYPLIEAPKALREVGTRKTYGKTILIP"
     gene            11052..11543
                     /locus_tag="PLF66_09870"
     CDS             11052..11543
                     /locus_tag="PLF66_09870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012389287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HMZ64390.1"
                     /translation="MKNKADKTKVNPEIAKEMEIFNEFLKKKDLKVTSQRMLVAETIF
                     SIHQHFTADSLLELLKDHRDEISKATIYRILSIMVEAKLLAEHDFGKDFKYYEHIIGH
                     EHHDHIICTDCGRIVEFVVPQIEDLQQKIAEEKGFTIHGHRLNIYGNCKKKPDCEFWN
                     KKG"
     gene            complement(11757..12782)
                     /locus_tag="PLF66_09875"
     CDS             complement(11757..12782)
                     /locus_tag="PLF66_09875"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HMZ64391.1"
                     /translation="MLLSLAISSYSSLFADRKVARLVEPIRKKSPSYASTKPTEIKKE
                     EPKIQEPPKEEITNIERIYEGKEYVVRIRARKFRQGELMFVRLQSNNPNITIAQLQKY
                     NLFWLKKKVEMFVLNNEYMGFIPIHPELQAGTYELEIKTEEVADTYKVCPVEIEATKF
                     KETNVAETLKLPKRFAPKKGGDPAALKFIMDCERLKKTAYQSYSQPMFSQNFHLPAKI
                     KKITSNFYARRNYFTKKGKPHGGIDIRGASGDPIHAIQDGKVVISRPMYFEGIFTVID
                     HGSKIFSLYMHQSETLVKEGDSVKRGQQIGKIGSTGMSTGPHLHLGLRVDNTLVNPMS
                     IIQLKIF"
     gene            12975..13775
                     /gene="folP"
                     /locus_tag="PLF66_09880"
     CDS             12975..13775
                     /gene="folP"
                     /locus_tag="PLF66_09880"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HMZ64392.1"
                     /translation="MMIFGILNVTSDSFSDGGEFLDQEKAERKGMELISCGASVLDIS
                     AQSSNVNAIQIDPNLEWSRLENLISKFKELGYKISVDTYKPFVIERSILAKVDYINNI
                     NSFRDEESLAILNQYKSSIPNLVLMYSHNNGDKAAVNSNLSPENIMGEIYRFFDNKIS
                     QLQKINIPEDKMIFDPGMGLFLGSDPMLSMTVLKNINQFKNRYGRVLVSVSRKSFIGN
                     LLGGIPPKERGAGTLALEMYLYEQNIDYIRTHDVRQLQQALEIRKSLN"
     gene            complement(13767..15794)
                     /locus_tag="PLF66_09885"
     CDS             complement(13767..15794)
                     /locus_tag="PLF66_09885"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HMZ64393.1"
                     /translation="MLKSFGILFLILFQLIFIHCKKDSIVYKADKGVLNLQNWNSNQN
                     PVVNLDGEWEFYWNELFEKKDLIGKQPDFISLPSTWNEKGLKGSGYATFKLKILLPEN
                     ETSPTYAIRIKPFTNCRAFIDEMEISCMGVIGKDFNSSVPDSRPSLSSFHPNQKEIDI
                     IIQVSNYHHRKGGFLYSIYFGTETGLLKYANNIRNRDSFILGILFFVMLYHIGLYLIR
                     RKETLSIYFMLVVLCVFLRTASTGEKLIVDIFSVNYSVYVALEYITFFMVMPLSINLI
                     VLMFSIQIHKFTIPVLFFKSILLSFITIFTDANFYSNIVSFYLPIFILEFIFAYYLVI
                     QSVFQKKENSMLIFLGVSIMILFTINDILNTLEFIHTSYIAHYGLISVVFCKSLYLLL
                     RFVKAFKENESLTIELTKANERLEEKITERTKEIMEEKIIAIKANELKDKFISLLSHD
                     LRSPMGNAKMLITSAIEADDASEKDLSRNYLDLASKSIANAVEMTEKILQFSQTKSGQ
                     IELNIQNCYPSRIADKIFNMMLSKIREKKIQLLNFIPEEEKIETDEALLQEVLFNLIS
                     NSIKFSKPGDLISIRSEMNQSAYRILVKDTGIGIPEEIRANLFSRSIKTTTIGTAGEV
                     GTGLGLPLCKDIVKLLRGDIFLSDAVSDGTEFTIHLPIYFPNDSKENRYLI"
     gene            complement(16205..17308)
                     /locus_tag="PLF66_09890"
     CDS             complement(16205..17308)
                     /locus_tag="PLF66_09890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004479828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM43 family protein"
                     /protein_id="HMZ64394.1"
                     /translation="MAISSDGMSSSESVGFGSQFIDSFKGIVTGFVMFPLSIFLIYKV
                     ETCTQAGDAFKNAKPVSQMEEGKPVYITGKLSASPFGTKFVKDGSYIAIEESSEVYAW
                     DEEERTEGEGSNKKKIRECKLKWTTSPDNPSSFQLPACKAKAFHQRREKSVNMAANGG
                     KITADGKTYNVDLADVNLTSAVPSSTPSSEEIITNGYTLSGNYLYSSATCASNEVEGC
                     ERVSVKVTPVPSGDMTFLGKLNGDTLGKYTYKDDSFLNASVGDYAQTMAAVKSDDAAM
                     KWVGRICGFIMMWAGLSLLVGPVTMLLDFIPFVGTLGSSAIRFFMGVVAFVLTTITIL
                     LVKFWYIWLLLIIGGLGYGYYKKKNASATPQST"
     gene            complement(17631..18683)
                     /locus_tag="PLF66_09895"
     CDS             complement(17631..18683)
                     /locus_tag="PLF66_09895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HMZ64395.1"
                     /translation="MIKILLSPLIFLILFCSSEQSLTNAHYAEVNVLDSSFLFVRAEP
                     HFNATEIDRLKNKQRVIILLEDDKILEKNGVSGKYVFIEYRKDDHLLKGWVFDSYLIR
                     ITNSVEDEQIFDSVIILFSVIFISLIVIAFWKRQAVLIFLKEFYNKLIQKRLVLSRIQ
                     AKMLFKIIRVFIILGIITVSLYILSNTLYKNRYFFQVFSLSQNETIAMRYLITIEKIM
                     KNGSIDENSDYYMRMINITDTTDIDIAKAIASSEKFFQHFRNMQIKNDTSDDFLGMVI
                     ELFSNPTNFLGKLSENFQKSNELIELESEFISNNKIIISNYSNVVKILNRLTENEIDY
                     LYENKRLPIYLLDERE"
     gene            complement(18914..19525)
                     /locus_tag="PLF66_09900"
     CDS             complement(18914..19525)
                     /locus_tag="PLF66_09900"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HMZ64396.1"
                     /translation="MRYLFLFPVASIVIIATLAISLWALYKDESLIDKFILHPYGMIR
                     RNEYYRIFSHAFIHADLWHLFFNMFSFYSFAFYLEANLGSLRFFIVYFGSILASVYTC
                     IQKNKMNPTYRSLGASGGVSGVIFGFILFEPMAKISIMFIPIGIPAFIFAFLYLAYSY
                     YSAENQYDNINHEAHFYGAVAGLALTSAFEPAVLSHFIRSISL"
     gene            complement(19522..20661)
                     /locus_tag="PLF66_09905"
     CDS             complement(19522..20661)
                     /locus_tag="PLF66_09905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002727738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HMZ64397.1"
                     /translation="MDIVELEKGFSETEDKIKKLAAECGNGVEIIRGGGISDTIHFIG
                     DTRKISEKTGYVKELPGVSRIWNVSAPYKNIARIAAGKAGEVVHRQDRIVEVKGPDGL
                     VRKIGNGKHIFIVGPDSPQTLEQTLTIAKQAKALGEKYGILDRMIIRGGAFKPRTRPT
                     DWRGLGWEGIKMLDKVREETGLPYVTEVMDHTMADEVAKHADMIQIGTRNAQDFELLE
                     AVGKTMKPVILKRGFGNEAEEWFSAAEYIANQGNLNIILCERGVKTLFIKEGYCRNTP
                     DFNVITHAKKKTILPTIFDPSHVAGDDKIVMQNLLGCLPFKADGSITETLHEESFRKE
                     QMCDAAQALVMSVYEKAIQAILAYEQHINPYISEMDTYFQNRSKA"
     gene            20849..21877
                     /gene="galT"
                     /locus_tag="PLF66_09910"
     CDS             20849..21877
                     /gene="galT"
                     /locus_tag="PLF66_09910"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HMZ64398.1"
                     /translation="MSEIRQNIITRDWVIIATDRAKRPHEFTKKEIKKIEVPEYKANC
                     PFCPGNEHLATKEFLRIGEGDNWKVRVISNKFPALSPEGERFRWKVGINSTITGIGVH
                     DVIVETPKHNAITALQKVDDIYHLLLAFKIRYEEIAKDTRMETIVIFKNHGESAGTSL
                     EHPHCQIAATPVVPYNFRARIQEVMRYFDDYGDCIFCRTALDEIAAGTRIIAENVNFI
                     AFVPYAALSPFHIWIFPREHTSSYEDATLEELWDLAKIMKKVLSMLYFGLGNPDYNFT
                     IRSTPLAERRTKYYHWYLSIVPRITKQAGFEMGSGMYINPSLPEESAEYLRNVEIPID
                     ENSPADPD"
     gene            complement(21939..24113)
                     /locus_tag="PLF66_09915"
     CDS             complement(21939..24113)
                     /locus_tag="PLF66_09915"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HMZ64399.1"
                     /translation="MVNVSQKTILLVEDEMIIATIEMAQLQKEGYKVIHTPTGEEAIE
                     MVKFSPIDLILMDIDLGSGIDGTQTAEEILKSYDIPIVFLSSRTEKEIVQKTEKITSY
                     GYVVKNSGIIVIDASIKMAFKLHEANKNIKKEKEQLRTILQSIGDAVIATDKNGVILQ
                     MNTIAEILTGWSVSEARGRHLSEIFKIYNKFTKEPHPDPVSNALTENNIIYLRNHTCL
                     KSKDGKEYQISDSSSPIRDSYGNVSGVVIVFRDVSKEYEIQKIIENSDKKYKSIIDIS
                     PIPYAVNDNKQNIVLLNKAFTQTFGYTLGDIPTLSDWWPKAYPDPIYRQWVAETWQAR
                     VEKSIKENSNFVPMEIVIRCKNGTNRTIMATAATLDESDAGLHLVILYDITDRKKIEL
                     ELKRSSDLLEESQSIAKVGGWELDLITGTLYWTKETYILHDTNPDEFNPTVDAGIQYY
                     LPSSRDILEKALKDAIEKGIGYDLELELLTIKNRKIFVRTTCVTTVHENKTVKLTGIF
                     QDISDRKKADEKIQNLLIEKDTILKEVHHRIKNNMTTLYSLLSIQSLQSEDQSFKNLL
                     QDSASRVRSMMVLYDTLYRSEKTQSMSIKKYLPILMQQIIEIFPSQKKVKIESEIDDI
                     ELQAQSLTPLGIFINELITNTIKYAFPNRDSGLIRVTAQKKNNEVEIIYSDDGIGISP
                     SIQINNPQGFGLQLINLLARQLNGSVSIEPSDGAKFVLRFKI"
     gene            complement(24118..26952)
                     /locus_tag="PLF66_09920"
     CDS             complement(24118..26952)
                     /locus_tag="PLF66_09920"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HMZ64400.1"
                     /translation="MTSRNKKQTVLLVEDETIIALMETRQLEKEGYSVIHVETGEAAI
                     DLVNENRDQVDIILMDIDLGSGIDGTQTAKEILKTFDIPIVFLSARVEKEFVQKTEAI
                     TSYGYVVKNSGINVIDASIKMAFKLYEARKETEKQKKRITAIIQSIGDAVIATDHFGN
                     IIQMNAVAENLTGWNFVEAEGRELHEVFQIIDPKTRKIVTNPVELVLSSQKSVTLAEH
                     KILIGRNGREYKISDSGAPIRDGDENITGVVLVFKDITKEYEAQRRIEISENSLQEAQ
                     RIAKLGSWQINLKTREVHCSKQFYSIFEVSEDINQEEIIQKIRESIHLKNSDLFTNSE
                     NFLINKNYFFDHKIHFPNNKIKYINTRIEPVLDESSIQIAYRGIVQDMTDRMNIEIAL
                     KQSEAHLRAIVDATPSCIKLVDRDGTLVNINSVGLSMIDVKNAKDVIGKNVYQLIHPD
                     HRETFKKFNESICDGKKGSLQFKIITAKGRIKWFETNAVPLQLQSTNTTIQLAITQDI
                     TEEKKFEQALLESEELYHSMFEKTQAIKLIIDPTDGTIVDANSAAVEFYGYSIDQFKH
                     LKISNINTLPEEMIQEELRNAAKEKRSYFNFKHRLANGAIRDVEVYSSPMTVNGRKLL
                     YSVIHDITERKIAEMALIENERSMKILLDTMTEGIALNEIIYDQNGDMIDYKIVNVNK
                     AFYSTVDYEGTEVIGNVASKLYKISPEFMREFWRNHKNQKTTIFSEIWSPVKHKYFFV
                     ATSPFVNGKFVTSFLDITERKNAEKALEEANIELEKLSNTDDLTKIGNRRYFQKTFDK
                     ECARARRNNHPLSIILIDIDDFKKYNDYFGHLKGDECLKTVATTISGLARRAADVTAR
                     WGGEEFIVLLPETNLTEAIIVAENIRKTIQSLQIPHPNARAANVVTLSLGIASWESEP
                     PHEFIARADQALYHAKSNGRNRIEIAEELK"
     gene            complement(27160..27921)
                     /locus_tag="PLF66_09925"
     CDS             complement(27160..27921)
                     /locus_tag="PLF66_09925"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HMZ64401.1"
                     /translation="MEQFIPFVISIFIFLLLGLRIAQEYQRAIVFRLGRFQSVKGPGL
                     YWIIPILERQQTVDIRTSTVELEQQETITKDSVTIKVNAVLWFRVTDPEKAIIKVANY
                     NQAVYQFSVTALRNIIGQHILDEVLKEREQINATLQKIVDASTEPWGIKIEMVEMKDV
                     EIPEGMQRAMAREAEAIREKRARIIKAEAELEASIKLTQGAKEMEKSPFSLELRRMQM
                     LSEIGIDNNTTTIIMMPAEFINAAKNFADSVSQKK"
     gene            complement(27946..28635)
                     /locus_tag="PLF66_09930"
     CDS             complement(27946..28635)
                     /locus_tag="PLF66_09930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016549999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase CarF family protein"
                     /protein_id="HMZ64402.1"
                     /translation="MFEYKHRHLEIASIFIFIVYCSMLVIQIIQETIRLADNLSYLVI
                     PLSIFPFFLGYIGADFISGFVHFLGDTFGTENTPVLGQSFIKPFREHHVDPKGMTKHD
                     FVETSGNNCIVSIPALLAVYYFVDIKANLFTYSFYSFFSFLMIAVFITNQIHKWAHED
                     NPGKIISFLQRNNLILSPKHHQIHHTEPFDKYYCITVGWLNPFLYKIHFFESVKNLAY
                     KVFGDRVAIKH"
     gene            complement(28647..29516)
                     /locus_tag="PLF66_09935"
     CDS             complement(28647..29516)
                     /locus_tag="PLF66_09935"
                     /inference="COORDINATES: protein motif:HMM:NF016103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HMZ64403.1"
                     /translation="MRKQIFFLSILLFTISLFGQEGYPKIFDSAAGKEISYESIAEKL
                     NPVDVLIIGEEHDDKKGHEEKLKLIRYLSERFTFVISMEMFERDQQIILNEYLAGLID
                     DKIFQTDLKLWNNYDDYKPIVQYARENKISILAANAPRRYVRILARNGLEEMYKFPSL
                     SRKFFAPIYTIEKYRQENYEHKIFGSLASHGKDTIGLKNMILAQNLWDATMADSILKV
                     LDKKKTKIIHINGRFHSDEYMGVSHRLQQLGVKILTISMFQNRETENIELSHFKKFAD
                     IIYLTGSAIPKKE"
     gene            complement(29513..30337)
                     /locus_tag="PLF66_09940"
     CDS             complement(29513..30337)
                     /locus_tag="PLF66_09940"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003001873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HMZ64404.1"
                     /translation="MKEDIRKRFEQIYAFLPKIQAFLIHTQNKDSFEIQLKGPIDLVT
                     EADKGSEKMLVQEILKNFPDDHILGEEGSSIKGKNQYRWIIDPVDGTTNFAHRLPLYG
                     VCIGLENIENGHMEMGLVSFPAMSDVYYAIRGYGAFKNEKQIYVSKTNELINSLASTG
                     FPYEKKARMESVIQNLKSVLLSVRDVRRTGVASLDLCWVAEGRFDAYWEENLKPWDMA
                     AASIIVEEAGGRITTYDGNNFSIFMPNLVATNATIHRKFLEKLSIISPQILSEFRI"
     gene            30487..30852
                     /locus_tag="PLF66_09945"
     CDS             30487..30852
                     /locus_tag="PLF66_09945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015677288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HMZ64405.1"
                     /translation="MEKQIMDILNVGIGLLQAGKEGLERAKTELEKTYTELSTKGAQD
                     NSEGAVQIRQSLDKIIHDIKEFTSVAGKNYEDTRSKIIDNYNKITEEIQNKMPEGKVE
                     AVKAKISEVAENIKKAKKA"
     gene            31109..33145
                     /gene="metG"
                     /locus_tag="PLF66_09950"
     CDS             31109..33145
                     /gene="metG"
                     /locus_tag="PLF66_09950"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015677505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HMZ64406.1"
                     /translation="MNKRKLLVTTALPYANGPIHLGHMLEGVQTDIWVRYQKLSGNDC
                     YFFCADDTHGTPVMLAAKKENISPEALVERIHSEHYRDLKGFYIEYDNYYSTNSPENR
                     YFSEEIYNNLKKNSHIAEREIEQSYCDHDKMFLPDRFIKGTCPKCKTKDQYGDGCEVC
                     GSNYSPKDLIDSHCAICGNTPGLKKSKHLFFKLQDFQKELSDWIENPEHVNAGVRNKL
                     NEWFSQGLQEWDISRDGPYFGFEIPGETNKYFYVWLDAPIGYMASSKNFFKDNALFDE
                     FWKSGDGEIIHFIGKDILYFHTLFWPAMLLGGGYKTPNRVNVHGFITVNGEKMSKSRG
                     TFIKASTFLKHLDPEHFRFFMAGKLNDTLDDLDLSFSDYMSKVNSDLVGNFINILSRT
                     GTSILDKLDRRIGNLNSEGKALISELIQSKSDILKNYEDKSYSKVMKEIARLGDLVNK
                     YVNDKAPWNLVKTDKEAAREVVTAAINCARILAIYLQPVIPKICEKLFKILDLPAQIS
                     FSELESVLENRNINAYEILSQRVDEKAVNSMLEENKEAQKESKTETINTKKTETNSNS
                     DSKLGLISIDDLAKVELRVGLIEEANHVTGADKLLNVKVNLGEKGIKNVFAGIKSAYK
                     PEELVGMKVVVVANLEPRKMKFGLSEAMLLATGKDESLSLIVPHRNAKPGDILK"
     gene            33151..33603
                     /gene="ybeY"
                     /locus_tag="PLF66_09955"
     CDS             33151..33603
                     /gene="ybeY"
                     /locus_tag="PLF66_09955"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HMZ64407.1"
                     /translation="MSLIKNIYIENNSHHRLGITPKEIKASIKKVVDHMTNLKGYELS
                     LLVTSDEEIQKVNLAKRGKDTPTDVLSFPIVEKDLPEGYKILGEVIISIDTMRKQARE
                     IGHSDKEEFYRLLVHGILHLLGYDHEKSAYEEKRMQEKEDECLKLILG"
     gene            complement(33600..34760)
                     /locus_tag="PLF66_09960"
     CDS             complement(33600..34760)
                     /locus_tag="PLF66_09960"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused response regulator/phosphatase"
                     /protein_id="HMZ64408.1"
                     /translation="MNESDFSILLVDDDELVLLSLELILSDKGYRVFNTSHSENILEI
                     IEREKIDLIVLDYYLYGVNGVEILSKIRSVELFAHIPILMLTSEESPDIIENCLDSGA
                     TDFIIKPVIPKVLLARIRSALSNREYILQIESQKKEISDSKEKLSEALASLEKDLRKA
                     RNIQETLIPKNFISSRSCKMFNYYKPMNEVGGDFFSFLEKEDTIDLLFGDVSGHGISS
                     AMVSCMAVLCFQTMDRNNQIEEEFKYLHQTLFSYVKGHYITGIYLRLDTKNKILQYAY
                     AGHHPAILIRNNQLIELEGKGTPLILMREFITASNSIQLEKGDKIFLFSDGLYEIYNL
                     NNEFYGSEQFLEKVKDMCHLQGNDFLKSIFNFSINYSGNVVKDDMTLLLIEI"
     gene            34946..37486
                     /locus_tag="PLF66_09965"
     CDS             34946..37486
                     /locus_tag="PLF66_09965"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HMZ64409.1"
                     /translation="MCAKYKSFYYFICSLLLFSSISIGSDENKTLNDNSILARKINLD
                     SESDWEYSLDDITDPTQFESNLNSTHWKKYKVPSNLNSIEINSKQRIWIKRKIILPEN
                     LSNKHLSIRLGSISDRDRTYFNGVLIGSTGCFGCDSPQAYDKVRIYDIPNNLTQKENI
                     LLIQVERFFPEEIGINQDYTAIGITSYLEYDRYKEDLFKLILLSIYGTVGCYFLFLYI
                     RRRQEVENLYFSSFVFTVIVYQFLRTQIKYQLNLDFVILKKIEYMFFASLIPLFSNFI
                     RVYFKIKISRFVYLLNLFSLFSIGFYLLSSNLVLFDLYNKRLTQPIGFLYIGFLFYYL
                     LNAFRSKNRDAVYFLSGVVLIFISVVTDILSDRGYFQFPRTFGYSFMFFVLSIAIILA
                     NKFVRLHSEVEELNTSLEQKVKDRTKELNESMEEIKQIKTQQDGDYFLTSLLLTPLTS
                     NRNQSEYVKTQFFSKQKKSFHFKNKTYEIGGDISISANITLNDKLYTVFINGDAMGKS
                     IQGAGGALVMGVVFNTVLSRSQVGSFKTKTPEKWLKDAFLELQRIFESFDGTMFLSCA
                     MGLIDEEAGFLYYLNAEHPWTILYRDGKARFIEENFTTRKLGIFENDKLFSVKTMKLL
                     PGDVLILGSDGRDDIQIGIKDDIRIINEDENLFLDIVQKGNGKLADIVELLHKTGELT
                     DDCSLVRVEYLGMPVNGKVNFTEILSRLFKSHYDMRNENMEDNVHLLRSVYKLHPSNE
                     VVNRQLSFHYFKKRDFHTAIKHINQYTYKKPESTDMFYLASVLNKKCGNYEKAIDIGE
                     QILLRDPKNFRNLKNLKELYHITQKMDRVDELERRIKELKNEVFRVKS"
     gene            37461..38372
                     /locus_tag="PLF66_09970"
     CDS             37461..38372
                     /locus_tag="PLF66_09970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010570355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HMZ64410.1"
                     /translation="MKYLELKVNLPKEVSEEFTAFLDSLSVEGYYEILFDSTLPRPAS
                     GEILRDDTNIHVYLGETDLEKEMKIYIYLKAVAGDKSFVESRIVETKEFEEAYKEFYK
                     PFQVGENFWIVPTWEKETEFTKNLVQRQNNIILFMNPGLAFGTGHHETTKLMIARIDK
                     IVQEGMSVLDLGTGSGILSIAAAYKKAKEILAIDIDPNAVRATEFNWKENSFPDPVQF
                     TVQEGGFDHPSIGNKHFDLLLGNITYAVISQNIEWIQKMNIKHFLFSGLIIERREDSI
                     RLFQEKLGGRLVYEEQLNDWIILEWER"
     gene            38360..38737
                     /locus_tag="PLF66_09975"
     CDS             38360..38737
                     /locus_tag="PLF66_09975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64411.1"
                     /translation="MGEINLHITEPIDCHLWKIRFPSVEDWPLDLKTLVVFEEDVHFE
                     RSLKQCDCGQLYFYEMIEFIDFERGEDPIYRTLIPIADEQTAVSLNSKSKFELINFLS
                     LRYDWPNNEKNARVYWHNRSETF"
     gene            38851..41976
                     /locus_tag="PLF66_09980"
     CDS             38851..41976
                     /locus_tag="PLF66_09980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004785063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HMZ64412.1"
                     /translation="MKSIISFFVYRPLISNLIIVFLLLAGSLSLLFIKRESFPNVNLY
                     QIKVTTIFPGASPADIEQKVTIPIEEKLREVSGFDSVRSISRNSESDINIKIDIDNKN
                     PDKVVDDIRRAVDKVTDLPVQIREKPLVIEQKSSDFPVLEISVYGGKDEIELQANAKF
                     IQDELRKVEGVGRVDAFAKRKKEWQVLVNPSSKQKYNVGFYDVILAIAERNVSIPGGS
                     INAETTKDIRVTGEFQEIGELGKIPVRANESGNQVFISQIARLKDTYEKERTIARTNG
                     HNAVNLLIIKKEQADIIRTIEKLNIKLQSIEKQIPETIHILELNNEGKKTINRLNVVI
                     SNSVQGLILIFIVLFVFFSLRDSILTSLSLPLSLLGSLLFMPILDISFNLISMLGIII
                     SLGMLVDNSIVISENIYNYRMKGMAPKDAAIVGTSELVVPIVGSYLTTIAAFIPMLSM
                     SGIMGKFVVQIPLMVIITLTMSLFESFFLLPVRIAKYGKIKTEVRESFFEKLRGKLDL
                     FFETMILKFESLTEKLVRRKYISLSIIASIFLSALVTISLMKFNLFPKEGIEQVIIKA
                     EFPPYFTARETLEKLQYLEPILDRIPKTEIIGYTVKVGIQQTDSGDPLTRVGEHLGMV
                     HLYFIPENERKRTTSEIIGELEAEIKKIPNAENVFIEELINGPPIGAAVTVAVEGKDY
                     KKLKEISNEIKDFLQSMNGVKNIADDYKKGREEIVLTLDSERAARAGISVATTANFVR
                     SAFEGNEASRFRLGKDEVRIKVQNDAQYRTSQEDIKRVNIINRYGLFTPLASFVNIKN
                     EQGIEALLHFDYEKAITITADVDEKIITSGMVNGSLAKQFGDLEKKYPGYSMKFRGEQ
                     ESTNKSMASLASAGIIAFFGIFAILAIIFNNTVKPFFIIMSIPLGLVGVIFGFLISNK
                     SLSFLAMIGIIGLAGVIVNTSIVLVDCIDELQKKGMDKYQSLVKAASSRFRPIILTTI
                     TTMAGLIPTAYGIGGTDAMLVPMTLALAWGLGFGTIGALFFIPTIFSIGYDFKSFLQK
                     QFRIYKGHEKT"
     gene            41973..42449
                     /locus_tag="PLF66_09985"
     CDS             41973..42449
                     /locus_tag="PLF66_09985"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HMZ64413.1"
                     /translation="MSSKIEYEIRQAQLEDINAIISLMIDFYKEAGFHLNIESTEKAL
                     QYLISHPENGFIFIAFRDRKPLGHIVLSKRFAMEYEDFIGYVDDLFVQKEFRRARIGN
                     ALLDKLCERAKELNLKSLQVEVGENNLPAIKLYEKIGLRAYQDGRVLLSKNLSRNN"
     gene            42527..43561
                     /locus_tag="PLF66_09990"
     CDS             42527..43561
                     /locus_tag="PLF66_09990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64414.1"
                     /translation="MNNLLLLIVCILFSMASCAEKKEKNKEIKLFSQIGFLSFATTNY
                     RCNITPSSNYAYFNSGTAPTGSSVSVRRIFSALRSAGTVEIYYPETVTKNLPVLVIYQ
                     GGNVNGSFYSNYAARLAASDYIVYLANRCTTFILQYFIYPDASLASEVYDLARTNTTD
                     SSSPLFGRIDTERIGVLGHSLGGVVGLFSINGICDFPFCARPTSRLTQIKAGLVYGSG
                     LGEGFDKKRFYTDSSGKGIPIGYMQGSLDTANTPDSSTKSYTNSISPKYLFSIDGLNH
                     YGITDVNNPFGAKSETNLSNLSQSQAIDRISNVSLLFLNAYVKNDSTSLSTINSGNLG
                     ITGVTTTASP"
     gene            43574..44263
                     /locus_tag="PLF66_09995"
     CDS             43574..44263
                     /locus_tag="PLF66_09995"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HMZ64415.1"
                     /translation="MNKKILVTGANKGIGLEITKQLTAMGHTVIMTGRSLSKLTEAKN
                     SLPLDAKTDFYEMDVSSGKSILDAYEKIKDVYPYLDVIINNAGILIDEDKSLFDSTED
                     DFLKTIQTNSIGPFLVIKSFSNLLKKGGTIINISSGGGQITHGVSTWSPLYCLSKTLL
                     NAITLQFAKALESKEITVNAVCPGWVKTDMGGQSASRSVEKGAETPVWLATSKVSFTG
                     KFLRDKKEIDW"
     gene            44429..45241
                     /locus_tag="PLF66_10000"
     CDS             44429..45241
                     /locus_tag="PLF66_10000"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HMZ64416.1"
                     /translation="MIFQNFKMYRIIAIIRSFIFLLVLLTLSLGAESVEEVKPWLGVA
                     IETAPNGVRVREAIVGTPAEKAGLKKGDVITKIDSIQMKESEQMIKYIRSKGVGNEVK
                     VEFERDTKKKKLAIVLEAKPDEFDVVKKQLLGKEVVDFKLTSLDGKKSYTKKDLENKI
                     TIFEFWATWCMPCRASHTRLSEFAEQNPSIQVLAISTESKDLIQNYLKEKNHKFTTVQ
                     DEKGDVSKFFMVSAIPTTALIDKNGKIKFLSLGGGIYLEETLKNALSLSKEK"
     gene            45260..>45618
                     /locus_tag="PLF66_10005"
     CDS             45260..>45618
                     /locus_tag="PLF66_10005"
                     /inference="COORDINATES: protein motif:HMM:NF016207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HMZ64417.1"
                     /translation="MRRSEFNMKEKEEIDSFLLKMSYGFLATHSSDEFPNLIPINYVW
                     FRDKFYFHGARTGRKMTEMKANEKVSFAIADEYSIIPSYFTDPKFACPATSFFKSVIA
                     YGVLEIIEDLDEKVLALT"
BASE COUNT        15003 a         7103 c         7871 g        15641 t
ORIGIN      
        1 gtattcctca ttgtctttca gatttacttt tgcctttttt ccaagtaaaa attttataat
       61 gtcgatataa cctttctcag aggctatcat taaaggagtg gttcctcctt tgtccatgga
      121 ctggtagtct acttttgcac ctttttcaat agcaagtttg actttattta aatctccatt
      181 ctcagatgct tcgtataacg catcattcca gttatttgcg tataaggata tatttaaaat
      241 aaaaaatgtg ataattaata attttttcat aattctatat agacacatgt gcctttattt
      301 tcttcgcttt ctattctgat tttaccctga tgtttgtcta ttatttcttt agctatggat
      361 agacctaagc caattgtctt ttctcctttt gtgccgattc ttcttgcgcg actaaatttc
      421 tcgaacaaga atgattgagt ttctttagga ataccaattc catgatcttt tatactgatg
      481 acagtgattt gtttttctcg atagagatga atttcaatga ttccattgtc ataagaaaac
      541 ttaattgcat tggaaataag attcgagatc acacgcatca ttttatcttg attgatattg
      601 tgaaaaactt tttcaatgga tgagtgatat tttattgaaa ttgatttttc tttgcactga
      661 aattcgtgca ttcttataca gtcttgtaag aatgtattta agtttgttaa ttccatatcg
      721 atcatttgag gatcttgttc tagttttacc atttctaata aatcctgtat gatactcatg
      781 ctatgatttc cagcattttc gattaactct aagtatttat atttagtctc atctgattct
      841 tgttttagta aaagccttgc taaattcgta atgccactga gtggagaacg aaggtcatgt
      901 gcgacgatat ttaaaattgc atttttagat tgatctcttt ttaaaagttc ttcttctgat
      961 tttttcctat tttctatatc aatggcaaaa tctagtctat aaatcacatt cccgtagttg
     1021 tcttttactg cgttggaata gcagccaagc cagattggat gaccatcata ggttatgcat
     1081 ttttgctcaa tatagaatcg attgatctgc ccatcaaata atctatggtc tgcatcttgt
     1141 ttgtattcca agaaatcttt ttgaaggaag tctgaatatt tcttacccac gaattcattt
     1201 cgtttatagc ccattaggtt taataaatag tcattggtgt ctaggattgt tccattttta
     1261 tctacaatga cgattcctat tggcgcattt tgatagagca ttctgtattt ttcttcactt
     1321 gcggctaact gcctttctgc tagttttctt ggtgtaatgt catttcgaat tgagatatat
     1381 ctaaatggat ttccattttc atctagtaag ggaataatgt atgtgtagac ccagtattca
     1441 gatccatctt tccgagtatt atttatttcc ccttcccaaa ctttaccaga tgatatctta
     1501 tcccaaagat ttttaaagaa ctgtttagaa tggtatcctg aattgattaa gcgatgatcg
     1561 tttccaataa tctcctcttt tgtgtatcct gagattttac taaaattatc attgcagtcg
     1621 ataataactc cattaatatc tgtaacggaa acgatagacg attgatccag ggaatatcgt
     1681 aagtcttcta atcttttatt ttttgttctt tgatctgtaa tatccataat cattccatac
     1741 gcactatgat cctttgaatt ggattcgagt aatatggaac gttcttctag ccatttccag
     1801 ccaccggttt tattttttac tctatactga atcgttctaa aattattatt ctttttaaat
     1861 agatctaggc ttgtgctaat cgatagtaaa tcttctggat ggatatgatt ctctaatata
     1921 ctaatatctt ccatgatttt gtgattaggg actcctaaga tagcctcagc tccctcgcta
     1981 ataaaaatca tttttcgttt ctgatttgtt tcgtaacgaa catgatagat gactccacca
     2041 atcgcattga acactgcatt tagaatttct tcactggaaa ttttctcatc tatcacattt
     2101 tgaataaaaa atgcagcgct ttggatattt gtatcaagtc gaagaagctt acgaatgatt
     2161 ttaactgtaa gagatttttt gtttttgttt tctagggtaa tcgttatact atcattggct
     2221 gtatttgtga aaaaactgta atactcattt aaagatattt ctttttcttt tggattttta
     2281 atatgaatcg taaaattttg attgattagt tcttctttac agtaacaaaa caaagaagca
     2341 tacgcactat tcactttgat ataatttcca ttaccatcta agtatgcaat gggaactata
     2401 gattcattga ataggaaatc ggattcagac atgtttacta cttatccttt ttgaaaaaag
     2461 tttaaaattt ggataataat tgttatccaa agaatcagat gaacttaaaa agtctagccg
     2521 ctattctaat taaccaaaaa ctactgacaa ctcatttgaa acttgcatat agaaaataat
     2581 tgtttctata ttttagacga taaaaaaatt aaaatgctat agtgctttat catctatcgc
     2641 aatttcgaat tggtattggg ttttttatag acaatatgta aaaaaaacag acatatacga
     2701 atgttgatat gctggaagga ttaagattca actatcgtat tcttccataa taacctggtt
     2761 gtaaacttcc ataaggatag tatgcgccag gagctcttcc tccatttaca gttcctaaac
     2821 ttcctggtgt ataccaagga ttttttatat tattggggaa gtaaagataa taggttctgg
     2881 aataattttg ccaagaatta gaataactga tacgtcttgt tactacttct aaaggttctt
     2941 ttagtctgtg atattctgca agttcttctt cggtcggatc acaatcaata cactcaactt
     3001 tgctctttaa ttgtcttagc tttgcagctc tatagggatt ttctggagtc tcttctgttt
     3061 cttcgaaaat agtagcacat tcggtaacca aacagatcgc taataaaaaa aataaaattt
     3121 tcattttctt ttcaagtttt tgattccaag cattcacttg actttccttg tgaaatttta
     3181 gactctgtta gaatctttca cacaaatata aatagtattt ttaaataatc tatgattgag
     3241 aaaaaaattc aaaccaaaaa taacgaagaa gtgaggattt atgtccttgg aaaaaaagaa
     3301 actgaacaat tagaaggtgt gccaaaggag aatttggctg tagaaattca aaatcttatt
     3361 cattctagat tttcattccc gaaaaataaa atctttttac tagatcaaac acatagtgat
     3421 ttgttctata atacaaatca aataagacca aatcaagaag gagatgcctt atatagttgt
     3481 aatttagagg agattcttgt tgttaaaact gcagattgta tgcctttatt cttttggtct
     3541 gaaacagaga ggctaatcgg cattatccac tctggatgga aaggaacaga ccttggaata
     3601 tctgaaaaac tttttaaatt tctaaaatcg aagggacata aaatagaatc gattcaagga
     3661 ttcttaggac cttgtgcgag aaaaaaaaat tacgaagtag gtcatgattt atttgaaaga
     3721 ttctctcttt atgcaccgag tgcaattgaa ccaaaagaaa atggcaagta tagtcttggg
     3781 atagacttag ttttaaagaa gcgtttgcag gaaaatctca ttgatatttt gctagaagat
     3841 tccgaaattt gcacaattga aaatacagaa tatcacagtc atcgacgtaa agacactgga
     3901 cgcaatctaa atatcattta cagaagaggt tgacatttat tttcaatatt gtatattgga
     3961 agagagtctg tctaattttc tttcgctaga aagaaaaacg gaggaaccaa tttttggggc
     4021 aaacttattt tagataataa gggaacatct ctcagattcc agcccgtcag ctaacttcgt
     4081 agaccaagag agaagatcaa atcctttttc taagactcac ttacaacaac tgatttgtat
     4141 tcaagttata ggtttttatt atttccatgc gatagatggt ataggaaatg catgcttata
     4201 gatctatagc cacatactaa ctcgggatat aaaattatgt aaataaggag atacttatga
     4261 aggtattagt tgcttttaca aaaccagaca cggggagagc tatttctcaa ttagcctcaa
     4321 aattggaagg cattcgggaa attcaagttg ttacggtttg gaatcaaaaa gagatatcca
     4381 taccaagtga attgaatcgt gagttagacg aaatcgcatc aatgctagtt aactcatcca
     4441 ttccttttaa aatagaaatg atctatgggg aaaacgttcc tttggaaatt caaaatgcag
     4501 cgaatcgaat gaatgcagat gcagttttat taggtgctgc caatactgtt ttttcttctg
     4561 acttatttgg aggaagagtt gcagaagtaa tgaaaggttt ttctagacct gtttacattt
     4621 tagcaaataa gaatctatct tatccttttg atcctgttct acttacttgc aatgatgctc
     4681 cttataactt tttggagaag aatgagcctc taagtggact atctcttgat tcgataccac
     4741 ttctagcaaa tcaagtagtt cctcaaaaat ctagttattg ggatttaaaa ggagagtttg
     4801 atttagataa acgttggctg aaaccttata atttaattgt tacagactac aagacgtatt
     4861 cactttacga tgattttttc ataaaagagt ccgatcagtc ttgtcttgta tattgtaatt
     4921 aagctaattt ttaattctct tttttataac taatctttta ttctgaattc ctctttccac
     4981 ggattgagtg aattcagaaa agactttaga atccaaatct aatttatcct ttagttttaa
     5041 aaaaagagga ttctcaggat ttatcgactc catttttatt tttaagtctg tttcgttaaa
     5101 cagcctaaat aatactgtgt ttttatcttc atcaatcaat gcgatagccg ctccaacttg
     5161 ttcctgcggt tcgattgtat caatcaccca accacccgtg tttataattc cgatggaatc
     5221 tgaatatcca taatttttaa atccagttct tctctcacaa aaaggtttat gcgtatgacc
     5281 aaaaataaat tctagatatt gagggatttg atttttgttt tcttctaaat attgattctt
     5341 taatggtcct gaaatatatg catagagtcc ttccaatgca tcttttccga ggatatccgc
     5401 tgttagcccc cgttcattga ttgcaatttg gcttacaatt ttagtcaaaa tataactagt
     5461 aattggcttt aacattgttt gtagagatgc tgggaaatca cttctcttca ttaaataaga
     5521 taccaagtta tttgaaagtt cgtttaaaga tttttcatct tgcaacatat catacatgat
     5581 gccaatactt gttcctacat tacccgaacg acccagcatt gaccaaaaaa aatctatcca
     5641 tgcaaaattt tctctttcaa tttggtcaat cgattctgga gagttttttg agttcagtaa
     5701 aatggattgc aagttactca ttaaaaaata aatcttttca ataaaatgtc catgggaaat
     5761 tgtaatgtat ttttctgtat ctctatccac taaagttaaa ttagggtaaa cagtcattac
     5821 tcttccttct ctcaaatgct tatgtctttg cattagggtt gaaataaatc tagatttaag
     5881 aggagttggc tttatcattt tagtatgatg ccatggtggc tcaataaact caccagggtt
     5941 ttgcttaatc atatattcta gatattgtct ttcgcgtgcc gtctcccaga gatgatgatc
     6001 gtgattccca gggatgtaaa ttaacttgtc cgaaaaaact tcttttctag atttagtgaa
     6061 tgcaagatct aaaaaacaat caaaggtcat cactgattcg tttgtgttcg ccagtgctaa
     6121 ttcaaaaata tctccattga gtattagttt cgcttttctt tttttatttg tctttgtaat
     6181 gatttcttct agtagattta aaaattgaac taaaacatcc gaactttttt gggggtctag
     6241 tctgccttca ctatcagtaa aagtcagtaa gctattatac gctccgaaat gtaaatcgga
     6301 caaacagatg tattgaatat cagccacaga gcaataatct aatgattcca ttcaagtgca
     6361 agaaggataa caaaaatcct aaactgattt tatatctgaa ttttcgggta gtaagaattt
     6421 ttagaactcg aaacgaaatt catttaaagc ctagaaaata gaagcaaaag ttgtttgctt
     6481 tgagcattga aaataaaata aaaaagattt gacaaaagct attggtgtta gctatttgat
     6541 ttatgaaagc taatggtagt agctttcata aaaggagatt tatatatgag aaaaattaaa
     6601 actgtatttt tgattttagc acaaattatt ttattacagg attgtagcac ctatgttccc
     6661 aaacttacag actcaggatt ttctgattcc agagagctta gcgaatcaca acttttaaaa
     6721 ccaacttcta agaaattagt agagctcatc agctttcata ccggtgaaat tgaagtagac
     6781 acaagcattt tactttctct gaaaaaagag aagaagctta agaataaaaa attttatgtt
     6841 cctgttctga gtcatctagt caggcattca gagaaaggag atattttaat tgatacagga
     6901 atggatgaat ctttttctaa atcaggtcat ggaaattttg gatgccttgc aaagcttgtt
     6961 tctttcgcaa gacaaaagcc tggaatggat ataggagctc aattaaaaag taaaaatgca
     7021 aatctatcgg ctgtttattt tacacatgcg catttggatc atacatccgg tgttcctgca
     7081 ctacctaaaa acattcctta ttatgctgga aaaggaacga tagatgattc ctttgcggat
     7141 atatttggag tttgctcgaa tcatttagat ggaatcgatt ccataaagga gattgatttt
     7201 aataaagcgg ctatcgttaa agaacttgga aaagttttgg atatttttgg ggatggaact
     7261 ctctgggcaa ttcatactcc tggtcattcc gcaggacata tgtccttttt agtaaataca
     7321 aaggatggtt cttatctaat tacgggagat gcgagtcaca cagggtatgg atttaaaaat
     7381 aaaatccctc caggaaagaa tgtaaattta gaacagacag aagaaacatt aaataaatta
     7441 atacaatttg taaatagaca tccagaaata aaactaatct ttggacatga tattcagaat
     7501 acattccagc cagaggtgga ttaaatgcct agacagggtc tcaatcgaaa ttcagtcatt
     7561 gaagcagcta tcgatcttgc tgaaagagag ggatttaatt ctctctcttt gggagagctc
     7621 gcaagacaat tagatattcg tcctccctct ctttataagc atatagcaag tttagaagat
     7681 ttacaagatg aaattggaat tagaggaatc gaagccttaa tcgaagcttt gagtaaagcg
     7741 gatactatca ttaatccgca ggattcaatt catgctatat gcacaattta tagaaatttt
     7801 gctttgaaga ataaagcttt gtatgaagcc attcaacctg ccatgaatcg gaggagcgcg
     7861 aaatttcaaa aatcagcaca gaaattattg gaaactattt tagtgtatgt catgaaatta
     7921 aagataaata aaaaggatca gatacatgtt ttgcgatctt tgcgtagttt gattcatggt
     7981 ttcattgaac tagaaaggca gggaggattt gggatgaaag aagacttgga taaaagcttt
     8041 caatatttga ttcagatttt tatagaaggt gttgtccgag tttagtgtat gctaaaagtt
     8101 tttattttat tgtcatattt ttctttgtta cctttatttg ctgaatctat taagatagga
     8161 attctttcca aatacaattt atcagagata gatttagata tctctgataa tactagaatg
     8221 ttttatgatg ggagagattt aaaactaaag aaaggtttga tcaaagttta cagtcaaaaa
     8281 gataaaatac attttggaaa aagcattatc gcaaagagta ttttaatcaa tgctagtgaa
     8341 aatataaaac tatccttctc aaaagagaat catgttttag agagaaatta taagggagat
     8401 ttagaattca taagtttaga tggaaggttg cagcttgtat tatcaatacc attggaagag
     8461 tatgtgtcgt ctgccgttca atctgaatta ggtgagcttt tgcaagatgg aagatacaat
     8521 tcagaggcac ataaaaaaga attaatagcc gcgcaagaaa ttgtaattcg cacattcata
     8581 aaaaaagaaa aggacaggca tgaaaaagaa ccttatgatt tttgtgatct gacccactgt
     8641 cttcattttg aaggaactca aaaaactaaa agtatttacc ctggagtcat attaaaagga
     8701 aaagattcta ttagcggtta ttttcattct acctgtgggg gaattctctc tggaccagaa
     8761 gtttattggg ctaagcatga aatttccact cattacaaaa gaggtaaaga tggagtatcc
     8821 gcttattgca gtgattctcc tcattcagag tgggagacaa ttctaacgga aagggaactg
     8881 gaaaatattt taaacgatag gaaaatctct aatatctttg tcgttgaaaa acagaacaga
     8941 gtcagtcatt taaaatacaa atcagaacaa tttataaaga cgatacccat atcaacattt
     9001 atttctagaa ccggtaaact ctatggctgg aataaaatta aaagtaattt atttaaaatt
     9061 cagaaattaa gagaaagata tttatttgtg ggaaaaggtc taggacatgg aatcggactt
     9121 tgtcaatggg gttctgctaa gctagcggct aacgggaaga cgtatcgcga aatattggag
     9181 ttttattttc cagataccga gctatctaag caatgaaaaa caagatcaaa atcattgtcc
     9241 ttttattttt aacagttcaa gtatgtttag caaaaccaac gttattcaag ttagatcaag
     9301 ttcactattt ttatcatggg aattcctttt ctgaaaatag aatcgaatat ttccatgaaa
     9361 tatttaaaca aacctatgaa gaagtttttg cgaaatttaa ggagagaaag gtttatccga
     9421 ttcgtgtaga gatatttaca aatacaaaag agtttcaaga acatacaaaa ctggctccaa
     9481 tttacgcagg tctctatact cctaaagaag gatttatttt tttactcaca cctaaaacaa
     9541 tgaatagaga tcgactcata caaattattt cccatgaatc ctgtcatgct cttctcgatt
     9601 taacaaatga ggaaatagat agggttttag aagaaggatt ttgctccaat gaatttcctt
     9661 ctgaagagtt tttgtatatt tggaacagga ctttattaaa cttagaattg gaaaaatttt
     9721 tagaaacagg aaattggatt ttaaaaaact cgagtcaggc aaagaagaga gatgtttacc
     9781 atgcggcaac tttatttatc cgctcgataa ggaaaacaaa aaatacggag gaagttctga
     9841 agctcctccg tgcgaatagt ctgaggaatc taaataaata ttggcacgaa ttcacttata
     9901 accaacgatt agagcagcag aataacaaac attagccgat tattaattgc atcagttctc
     9961 tacaagatca ttcatgtatt acttatattg attaatgtca taaggatgac atttatcaat
    10021 tacaagatta ttacggaaca ataaaaaaag attttaaaaa ttaaatgtct agatgagatt
    10081 gataatatga aagcttacgt tgcagagaat tggtgcgagc cagaagaact tgtttttaaa
    10141 gatgttgaag atcctacacc gaaagaaggt gaagttgtgt tggatattaa agcaagtggg
    10201 atgaattttc ctgatatgtt actcatccaa ggcaaatatc aaatgcgtcc taaaagaccg
    10261 ttttctccag gaatcgaagt ggctggaatt gtatccgccg ttggacccgg agtgacaaag
    10321 tggaaaatag gcgataaagc gatgggttta tcttggttag gtggatatgc agagaaggtg
    10381 gcagtagcag aaagaaatgt atatcccatg ccagatggaa tgagttttga agaagcagct
    10441 ggatttattg ttacatacca atcatcttat tttgcaattg tcgttcgcgc gaatctaaaa
    10501 ccaacggaaa ctatgttagt gcatgccggt gctggtggta taggaagttc tgcgattcaa
    10561 attggaaaag ctatcggtgc gaaagtatat gcaacggctg gaagtgaaga aaaattagac
    10621 gttattcgta atctaggagc tgacttagca ctaaattaca atgatgcaac ttggtcaaaa
    10681 aaaatcagga aagagtatgg tggtgtcgat gttgtaattg atccagtagg aggtgatgca
    10741 tttgataaat caattctttg cacaaacttc gaaggtagaa tcgtcgttgt tggatttaca
    10801 agtggtcgta ttccaactct tgcggttaat atgctgttac tcaacaacat cagtgtaatg
    10861 ggtgtttatt ggaacttata tcaaaacgat agaccccaag aaatcgaaaa atgtgtaaaa
    10921 cagttaaatg agtggtatac acaggggaaa gtaaaacctt tggtttataa gacttacccg
    10981 ctcattgagg ctccgaaggc tcttcgggaa gtagggacaa gaaaaacata cgggaaaaca
    11041 atattaatac catgaaaaat aaagctgaca aaacaaaagt taatccagaa atcgcgaaag
    11101 aaatggaaat atttaacgag tttctaaaga aaaaagattt aaaagttacc tctcagagaa
    11161 tgctagtagc agaaacaatt ttttcaatac atcaacattt tacagcagat agcttattag
    11221 aacttttaaa agatcataga gatgaaattt caaaggctac aatttaccga atactaagca
    11281 ttatggtaga agccaaactt cttgctgaac atgattttgg aaaggatttc aagtattatg
    11341 aacatatcat aggtcatgaa catcatgatc atattatatg cacagattgt ggaagaatcg
    11401 tagagtttgt agttccacag attgaagatc tacaacaaaa aattgcagag gaaaaaggtt
    11461 ttaccatcca tggtcacaga cttaacatct atggaaattg taaaaagaaa cctgattgcg
    11521 aattttggaa taagaaaggc tagttttaag aatatatacc aattcttaaa aggaattgcc
    11581 taaaaagggt ataaaaaaaa gagtctttga tttaaaaaga tcaaaggctc tttatgcaaa
    11641 gcggctaacg cttataaaag tgaatcaact tatatataga tggatgtttc taaaataaga
    11701 aactgattga atttaattcc tatctaatct atagaaaaaa ataattttaa ttatctttaa
    11761 aaaatcttta attgaataat tgacattgga tttactaagg tattatccac acgtaaacct
    11821 aaatgaagat gtggacctgt tgacatgcct gtagatccaa tttttccaat ttgttgtcct
    11881 ctttttacag aatcaccttc ttttacaaga gtttctgatt ggtgcatata gagagaaaat
    11941 atctttgatc catgatcgat tacagtaaag attccctcaa aatacattgg tctggaaata
    12001 acgactttcc catcctgaat tgcatgaatt gggtcgcctg aggctccacg aatatcgatt
    12061 cctccatgtg gctttccttt ttttgtgaaa taatttctac gtgcataaaa gttactggtt
    12121 atctttttta tttttgcagg taaatgaaag ttttgcgaaa acattggctg tgagtaggat
    12181 tgataggctg tcttttttaa tctttcacaa tccattataa atttaagagc ggctggatct
    12241 cctcccttct taggagcaaa tcgttttggt agttttaaag tttctgcaac gttagtttct
    12301 ttaaacttgg ttgcttcaat ttccacagga catactttat aagtatctgc aacttcttct
    12361 gttttaattt ccagttcata agttcctgct tgcaattccg gatgaatagg aatgaagccc
    12421 atatattcgt tgtttaaaac aaacatttca actttctttt taagccagaa taggttatac
    12481 ttttgtaatt gagctattgt aatgtttgga ttgtttgatt gaagacgcac aaacataagc
    12541 tctccctgtc taaactttcg agctcgaatt cttactacat attccttccc ttcataaatt
    12601 ctttctatgt ttgtgatttc ttctttgggt ggttcttgta ttttaggttc ttcttttttt
    12661 atttctgttg gctttgtgga tgcatagctc ggagattttt ttcgaatggg ttctactaac
    12721 ctagctactt tcctatctgc aaataaagaa gagtaggagg aaatagctaa tgaaagaagc
    12781 aaaattttag ataataaaaa aatagaataa ttttgaacca tgataaatgt aatctcgttc
    12841 ctaaaaaatt tttactgaca gataaaacta aaaagtattt ttttcaatat aaaaaatatt
    12901 aaaaagtaga aaagatacca ttctaataaa aaaagatagc ttattcctgt atttcttaaa
    12961 gttttagaaa tcctatgatg attttcggaa ttttaaatgt cacatctgat tctttttccg
    13021 atggtggtga gtttttggac caggagaaag cagaacgcaa aggaatggag ttaataagtt
    13081 gtggggcaag tgttttagat ataagtgccc aatcttcaaa cgtaaacgcg atccaaattg
    13141 atcccaattt agaatggtct aggttagaga atttaatttc taaatttaaa gaattaggtt
    13201 ataagatttc cgttgataca tataagccat ttgtaataga gagatcaata ctagcaaaag
    13261 ttgactatat caataatatc aattcctttc gagacgaaga gtctcttgca attttaaatc
    13321 aatataaaag ttctatacca aaccttgttt tgatgtattc gcataacaat ggagataaag
    13381 cagcggttaa ctctaattta tcgcctgaga atattatggg agagatctat cgcttttttg
    13441 ataacaaaat tagccaattg cagaaaataa atattcctga agataagatg atttttgatc
    13501 caggtatggg tttgttttta ggttcagatc caatgcttag tatgactgtg ctaaaaaata
    13561 tcaatcaatt taaaaataga tatggtcgtg tgcttgtttc agtctctcga aaatctttta
    13621 ttggaaattt actaggaggg attcctccta aagagagagg ggcaggcact cttgctttgg
    13681 aaatgtattt atatgaacag aatattgatt atattcgcac acatgacgtt agacaattac
    13741 agcaagcttt agaaattcga aaaagtttaa attaaatatc ggttttcttt agagtcgttt
    13801 ggaaaataga ttggcaaatg aattgtaaat tctgttccat cagataccgc atcacttaag
    13861 aaaatatctc cgcgtaatag ttttacaata tctttacaga gtggaagacc caagccagtg
    13921 cctacctcac ctgctgtccc tattgtggtt gtttttatag atctagaaaa taaattagct
    13981 cttatttctt ctggaattcc tatccctgta tctttaacta atatgcggta ggcgctttga
    14041 ttcatttcgg atcttataga aattagatca cctggtttag agaactttat cgagttagaa
    14101 attaaattaa ataaaacttc ttgtagaagt gcttcatccg tttcaatttt ttcttcttca
    14161 ggtataaaat tgagtaattg aattttcttc tctctaattt tgctgagcat catattaaag
    14221 attttgtctg cgattcgaga aggatagcaa ttttgaatat tgagttcaat ttgtcctgat
    14281 ttagtttggc tgaattgtag aattttctct gtcatttcaa ctgcatttgc tattgattta
    14341 gaagctaagt ccaaataatt tctagataaa tctttttctg atgcgtcatc cgcttcgatt
    14401 gcacttgtta taagcatttt agcattcccc attggagaac gcaaatcatg tgaaagaaga
    14461 gaaataaatt tgtcttttaa ttcattcgct ttgattgcga taattttctc ttccattatt
    14521 tcctttgttc tttctgtaat tttttcttcg agtctttcat ttgcctttgt taattcaata
    14581 gtaagtgatt cgttttcttt aaatgctttc acaaacctga gaagcaagta gagagattta
    14641 cagaatacaa cggagataag tccataatgg gcaatataac ttgtatgaat gaattctaat
    14701 gtgtttagta tatcatttat cgtaaatagg atcattatgc tgactcctaa aaaaatcagc
    14761 atagagtttt cctttttttg aaatacagat tgaattacta aataataagc aaatataaac
    14821 tcaaggataa agattggtaa atagaaggat actatattag aatagaaatt tgcatctgtg
    14881 aagattgtga taaaagatag tagaatactc ttaaaaaaca atacaggtat tgtaaatttg
    14941 tgaatttgaa tggaaaacat caacacaatc aaattgatag aaagaggcat taccataaaa
    15001 aatgtaatgt attccaacgc tacgtaaacg gagtagttaa cagaaaaaat atctacaatc
    15061 aatttttcgc cggtgcttgc tgttcttaga aatacacata aaacaactaa cataaagtaa
    15121 atcgataaag tttcttttcg cctgattaag tagagcccta tatgatagag cattacaaaa
    15181 aataaaattc ctaaaataaa actatctcga tttcgaatat tatttgcata ttttaataaa
    15241 cccgtttctg ttccaaaata gatgctatat agaaatccgc cttttcggtg atggtaattg
    15301 gatacttgga taataatatc gatttctttt tgattgggat gaaaagagga aagagatggt
    15361 cttgaatcag gaacagatga gttaaaatct tttcctatta ctcccataca agaaatttcc
    15421 atttcatcaa taaaagctct acaatttgta aaaggtttaa ttcgaatcgc atatgttgga
    15481 gacgtttcat tttctggtaa taaaattttt aatttgaaag tagcgtagcc tgatcccttt
    15541 agacctttct cgttccatgt agagggaaga gaaataaaat caggttgttt accaataaga
    15601 tctttttttt cgaatagctc attccagtaa aattcccatt ctccatctaa attgacaaca
    15661 ggattttgat tagaattcca gttttgtaaa ttcagaactc ctttgtctgc tttataaacg
    15721 attgaatctt ttttgcaatg aataaagatt aattggaata gtatgagaaa tagaatgcca
    15781 aaacttttga gcatagcata aattaactaa tctgattaaa tagtcaagaa ttcttgatta
    15841 atttttttaa atcgatatga ttttagaaaa aagcttcctt atattttccc atttgttcta
    15901 ttttgggaat taaaaccttt tatacataga atagagaagg aagaatttat ttttttgact
    15961 tttgttttta gattaatctt tcttttactc ttggttttaa aataccttaa attaagtata
    16021 tttactattg gatattgctt ttcgtgattt gcataaatta gttttaaaac aaatcttata
    16081 tatttgattt ttaaattgaa tttctttatc ttttatattt ttttattaat caataaagaa
    16141 atgtaaagta acgctttgtg aggcaaaacg ccgtaatccg ctacggcatt ttgcataaac
    16201 ttaattatgt gctttgtggt gtagcacttg catttttctt tttgtaataa ccataaccta
    16261 gtcctccaat gattagaagt aaccagatat accaaaattt aactaataaa atcgtaatgg
    16321 tagttagaac aaaagcaact actcccataa agaagcgaat tgcagaactt cctaaagtcc
    16381 caacaaaagg tataaaatca agtagcattg ttactggacc tactaataaa gataagccag
    16441 cccacatcat aataaagcca cagattcttc caacccattt cattgcagca tcatcagatt
    16501 taactgcagc catagtctga gcatagtctc ccacacttgc atttaaaaaa gaatcatctt
    16561 tgtatgtgta tttgcctagt gtatctccat ttaattttcc gaggaaagtc atgtctccac
    16621 ttggaactgg agttacttta acagaaactc tttcgcagcc ttctacttca ttggatgcac
    16681 aagtcgcact gctgtataaa taattacccg atagagtata tccattggta atgatttctt
    16741 cgctagatgg agttgagctt ggaactgcgg atgtaagatt cacgtcagct aaatcgacat
    16801 tgtatgtttt accatcagct gtaatttttc caccgttagc cgccatattt acactttttt
    16861 ctcgtctttg gtgaaatgcc tttgctttac aagcaggtaa ctggaatgaa gaaggattat
    16921 ctggacttgt ggtccatttt aatttacatt ccctgatctt ctttttatta ctaccttctc
    16981 cttcagtcct ttcctcttcg tcccatgcat agacttcaga gctttcttcg attgcaatat
    17041 aacttccgtc ttttacaaat ttggttccaa agggtgatgc acttaatttt ccagtaatat
    17101 aaaccggctt tccttcttcc atttgtgaaa caggttttgc atttttaaaa gcatctccag
    17161 cttgtgtgca tgtttctact ttataaatga gaaatataga cagagggaac attacaaacc
    17221 ctgtcacaat tcctttaaac gaatctataa attgagaacc aaacccaact gattcgctag
    17281 atgacattcc gtcactacta attgccatag gtatctccta attgaatttt ttacgaggag
    17341 ttattattaa gatttataga aataagacaa caagtttttt gagaatgatt gatgttaggt
    17401 gaaaataaac gatcgtcttt gctgaaagaa aagcaagatt gcgactaata gctcatatat
    17461 aatttccgaa aagctgaggt tataaaaatt ttaaatgctc aattttaaaa tacatactac
    17521 aaaaataaaa gaattcataa gaaggattag aaaccgttga ttctttattt ttttataggt
    17581 aattaatggt ataaaaattg tatggacaac gtaaaatttc actaggacaa tcattctctt
    17641 tcatctaata aataaattgg taaacgcttg ttttcgtaaa ggtaatctat ttcattttca
    17701 gtaagcctat ttaatatttt cactacatta ctgtagttag aaataataat tttattattt
    17761 gaaataaatt ctgattcaag ttcaattagt tcattagatt tttgaaaatt ttccgaaagc
    17821 ttacctagga aattggttgg attcgaaaat agttcgatta ccattcccaa aaaatcgtct
    17881 gatgtatcat ttttaatttg catatttcta aagtgttgaa agaatttttc agacgatgcg
    17941 atagcctttg ctatatcaat atcagtagta tccgtaatat ttatcattct catataataa
    18001 tctgaatttt catcgataga accgtttttc attatttttt ctatagttat taaataacgc
    18061 attgcgatcg tctcattttg cgataaagaa aacacttgaa aaaaatatcg atttttatac
    18121 agagtattgc ttaaaatgta tagactaaca gttattatac ctaaaataat aaaaacacga
    18181 ataatcttaa ataacatttt tgcttgaatt cttgaaagaa ctaatctttt ttgaattaat
    18241 ttattataga attcttttaa aaaaattaag accgcttgtc gcttccaaaa agcaattact
    18301 attaatgaga taaatatgac agaaaataga ataataactg aatcgaaaat ttgctcatct
    18361 tctacggagt ttgtgatcct tattaaatat gaatcaaata cccacccttt taataaatga
    18421 tcatcttttc tatattctat aaaaacgtat tttcctgata ccccattttt ttctaatatt
    18481 ttatcatctt ctaatagtat aataacacgt tgtttatttt ttaatcgatc tatttcagtt
    18541 gcattaaaat gtggttcggc acgaacaaag agaaatgaac tatctaaaac atttacttct
    18601 gcatagtgtg cgttcgttaa tgattgttct gatgagcaaa ataaaattaa aaatattaaa
    18661 ggagacaaca aaattttaat cataaaaact tcctattaaa aaagaaatac actagtttaa
    18721 tacaatagat aaatgttttc tatttaaatc aagaaccaaa aaagcgttaa aaataatttc
    18781 attgttgtca attttcattt atctcattct atacgtttaa cgtatagaaa aattatgaaa
    18841 agctttaatg agtctagata tattccctga aaatctgcga ggactattcg attctttttt
    18901 ttgccaaaaa ctactataaa ctaatagaac gtatgaaatg actcagaacg gcaggctcaa
    18961 aggcagaagt caaagcaagt ccagcaactg ctccataaaa gtgagcttca tggttgatat
    19021 tgtcatattg attttctgct gagtagtaag agtaagcaag ataaagaaat gcgaatataa
    19081 aggcgggaat tccaattgga atgaacatga tactaatctt tgccattggt tcaaataaaa
    19141 taaaaccgaa tattacgccg gagactccac ccgatgcacc taggcttctg taggttgggt
    19201 tcattttgtt tttttggata caagtataaa cactcgcaag tattgatcca aaatagacta
    19261 taaaaaatct aagcgaccct agatttgctt ctaagtaaaa agcaaatgag taaaaagaaa
    19321 acatattaaa aaataaatgc caaagatccg catgaataaa ggcatgagaa aaaatacgat
    19381 agtattcatt tcttcgaatc attccataag gatgaaggat gaatttgtca attaaagatt
    19441 catccttata gagcgcccag agactaatgg ctaatgtagc gattataact atggacgcaa
    19501 ctggaaagag aaaaagatat ctcaagcttt ggacctgttt tgaaaatagg tatccatctc
    19561 tgaaatataa ggattgatat gttgctcata ggcaagaatt gcttggattg ctttttcata
    19621 aacactcatc acaagagctt gcgcagcatc acacatttgt tcctttctaa atgattcttc
    19681 atgcaatgtt tcggtaatag acccatctgc tttaaatggt aggcaaccga gtaagttctg
    19741 cattacaatt ttgtcgtcac ctgcaacatg cgaaggatca aagattgtag gaaggattgt
    19801 cttctttttt gcatgtgtaa taacgttaaa atcaggagta tttctacaat atccttcttt
    19861 tataaagaga gttttcacgc ctcgttcaca tagaatgatg tttagatttc cttggtttgc
    19921 tatgtactct gcagcagaga accattcttc tgcctcattt ccaaatcctc tttttaagat
    19981 gacaggcttc attgttttac caactgcttc tagaagttca aagtcctgtg catttcttgt
    20041 tcctatttga atcatatctg catgctttgc aacttcatct gccattgtat gatccataac
    20101 ttccgttacg taaggtagac cagtttcttc tctaactttg tctagcatct taattccttc
    20161 ccaaccgaga cctctccaat ccgttggtct agttcttggt ttaaaagcac caccacggat
    20221 aatcattcta tctaaaattc catatttttc accgagagct tttgcttgtt ttgcaattgt
    20281 aagagtttgc tctagtgttt gaggagagtc gggaccaacg ataaatatgt gtttgccgtt
    20341 tccaattttt cttactaaac catctggtcc ttttacttca acaattctat cttgtctatg
    20401 gacaacttca ccagctttgc ctgctgcaat tcttgcaatg tttttgtatg gagcagatac
    20461 attccaaatg cggctaacgc ctggtaactc cttgacgtat ccagttttct ctgatatttt
    20521 tcttgtatca ccgatgaagt gaatcgtatc actgattcca ccgcctctaa taatttctac
    20581 gccattgccg cattctgcag cgagtttttt aatcttgtct tctgtttctg agaagccttt
    20641 ttctagttct actatgtcca aatttttacc ctcgaaatac aaagtcttaa taaacaaaga
    20701 aaagtcaaga atttgtattg aaattgaatc ttagatggat tgagttactg ataattatca
    20761 tgatttcaaa gggattttgc tgatttaaaa tcttgctttc ccagatttga atttttaatc
    20821 tagccagagt agtgtgagga atcgagttat gtctgagatt agacaaaata taattacaag
    20881 agattgggtt attattgcaa cagatagagc aaagcgacct catgaattta caaaaaaaga
    20941 aataaagaaa attgaagttc ctgaatataa agcgaattgc cctttttgtc ctggcaatga
    21001 gcatttagct actaaagaat ttctaagaat cggagaggga gacaattgga aggtaagagt
    21061 tatctctaat aaatttccag cgttatctcc agagggagaa aggtttcgtt ggaaggtggg
    21121 gattaattct acaatcacag gaattggagt ccacgatgta attgtagaga caccgaaaca
    21181 caatgcaatc accgctttac aaaaggttga tgatatttac catctgcttt tagcgtttaa
    21241 aatacgatat gaggaaatag ctaaagatac tcgtatggaa acgattgtga tttttaaaaa
    21301 ccatggagag tcggctggca catctcttga acatccacac tgtcagatcg cggcaacccc
    21361 agttgttcca tataactttc gagctaggat acaggaagtc atgcgctatt ttgatgatta
    21421 tggagattgt atcttttgta gaactgcgtt agatgaaata gctgcaggaa ctagaatcat
    21481 tgctgaaaat gtaaacttta ttgcttttgt tccttatgct gctcttagtc cttttcatat
    21541 atggattttt ccgagagaac atacttcgtc ctatgaagat gccacattgg aagagttatg
    21601 ggatcttgca aagataatga agaaagtttt atcaatgctt tattttggtc ttgggaatcc
    21661 tgattataat tttacaattc gatctactcc acttgcagaa agaaggacga aatattatca
    21721 ctggtatttg tctatcgttc caagaattac aaaacaagct ggctttgaaa tgggaagtgg
    21781 tatgtatatt aatccatctc tacctgaaga aagcgcagaa taccttagaa atgttgaaat
    21841 tccgatagat gaaaatagtc ctgcagatcc tgattgattt tttatctctt gcttaacgtt
    21901 agtttgatat ctgaatgctt cacgaattta attttgaatc aaattttaaa tcttaagaca
    21961 aactttgcgc catcagaggg ttcaatcgaa actgatccat ttagttgtcg tgctagaaga
    22021 ttgattaatt gcaaaccaaa accttgtggg ttattaatct gaatggatgg agaaattccg
    22081 attccatcat ctgagtaaat aatttcaact tcattgtttt ttttctgagc agttactcga
    22141 atgagaccgc tatcacgatt tggaaacgca tatttaattg tatttgtaat caattcgtta
    22201 ataaaaatac caagtggtgt taatgattga gcttgtaatt caatgtcatc aatttctgat
    22261 tcaattttta cttttttttg tgaagggaaa atttcaatga tttgttgcat gagtattggt
    22321 aaatactttt taatcgacat agactgagtt ttttcagagc gatagagagt atcgtataaa
    22381 accatcatac ttctgacacg acttgctgag tcttgtaata agtttttaaa gctttgatct
    22441 tctgattgaa gagattgaat tgataacaag ctatagagag ttgtcatgtt attttttatt
    22501 ctatggtgca cttcttttaa gatcgtatct ttttctatga gaagattttg gattttctca
    22561 tctgcttttt tccggtctga aatgtcttgg aagattcctg ttagttttac cgttttattt
    22621 tcatgaactg tagttacgca tgtcgttcta acaaaaattt ttcggttttt aatggtcaat
    22681 aactcaagct ctagatcata gccgattcct ttttcaatcg catcttttaa ggctttctcc
    22741 aaaatatccc gtgagcttgg tagataatat tgaattcctg catccacagt aggattaaat
    22801 tcatctggat ttgtgtcatg tagaatgtag gtttcctttg tccaataaag agttcctgta
    22861 attaagtcaa gttcccatcc tccaactttc gcaatggatt gagattcttc taatagatct
    22921 gaacttcttt ttagctctaa ttctattttt tttcgatctg taatatcgta gaggataaca
    22981 aggtgtaaac cagcatcgga ttcatctaaa gtggcagcag tagccataat agtcctattg
    23041 gttccatttt tacatcgaat cacaatttcc atggggacaa agttgctatt ctcttttatt
    23101 gatttttcaa ctcttgcttg ccatgtttct gcaacccatt gtctataaat tggatctggg
    23161 taggctttgg gccaccaatc agacaatgtt ggtatatcgc ctagcgtata tccaaatgtt
    23221 tgcgtaaatg ctttattgag tagaactata ttttgtttat tgtcatttac agcataaggt
    23281 attggggaaa tatcgatgat agatttgtat ttcttatcac tattttctat aattttttgg
    23341 atttcatatt ctttgcttac atctctaaaa actataacta ctccgcttac atttccatat
    23401 gaatctctga ttggagaact agaatcagaa atttgatatt cttttccatc ttttgatttt
    23461 agacaagtgt ggtttcttaa ataaattata ttattctctg ttaaagcgtt agaaactgga
    23521 tctggatgtg gttcttttgt gaatttatta tatattttaa aaatttcact tagatgtctt
    23581 cccctagctt ctgagactga ccatcctgtt agtatttctg caatcgtgtt catttgaaga
    23641 atgactccat tcttgtccgt tgcgattact gcgtcgccta tcgactgtaa aatagtcctt
    23701 aattgttctt tctctttctt tatattttta ttcgcttcat gtaatttgaa agccatctta
    23761 atagatgcat ctatgacaat gattcctgaa ttttttacaa catagccata agatgttatt
    23821 ttttctgttt tttgaactat ttctttttct gttcgggatg ataagaatac gattggaata
    23881 tcataggact ttaaaatttc ttcggctgtt tgtgttccat ctataccact tcctaaatct
    23941 atatccatta agatcaaatc aataggagaa aatttgacca tttcgatagc ctcttctcca
    24001 gttggagtgt gaattacttt atatccttct ttttgtaact gagccatttc aatggtggca
    24061 attatcattt cgtcttctac taaaagtatt gtcttttggg aaacattaac cattgtatta
    24121 ttttaattcc tctgcaattt cgattctatt tctaccattg cttttggcgt gataaagtgc
    24181 ctggtctgct cttgcaataa attcatgtgg tggctcagat tcccaacttg caatacctaa
    24241 gctaagagtg acaacgttag ctgctctagc atttggatgt ggaatttgaa gactctggat
    24301 tgtttttcga atattttcgg caacgataat cgcctctgtt aaatttgtct ctgggagtaa
    24361 tactataaat tcttctccgc cccaacgtgc agtaacatca gccgctcttc ttgctaatcc
    24421 tgaaatagta gttgcaactg tttttaaaca ttcatcccct tttaggtgtc caaaataatc
    24481 gttgtatttt ttaaaatcat caatatctat taggataatc gaaagaggat gattatttct
    24541 acgagctcgt gcgcattcct tatcaaaagt tttttgaaag tatcttctgt ttccaatttt
    24601 tgttaaatca tctgtgttag ataatttctc taactctata tttgcttctt ctaatgcctt
    24661 ttcggcattc tttctctctg ttatatctag aaagcttgta acaaatttac cgtttacgaa
    24721 gggtgatgtt gctacaaaaa aatacttatg tttgactgga ctccaaattt cagagaagat
    24781 tgtagttttt tgatttttat gatttcgcca aaattctctc ataaattctg gagatatttt
    24841 ataaagtttt gatgctacat ttccaattac ttctgttccc tcgtaatcaa cggttgaata
    24901 aaaggcttta tttacgttaa ctattttata atctatcatg tctccattct gatcatagat
    24961 gatttcgttt aacgcaattc cttctgtcat tgtatctaat agaattttca tacttctttc
    25021 attttcgatt aaagccattt cggctatctt tctttctgtg atatcatgga taacagagta
    25081 taataatttt cttccgttta cagtcattgg acttgagtaa acctctacat cccgaatggc
    25141 tccgtttgcc aatctatgct taaagttaaa gtaggatctt ttctcttttg ctgcatttct
    25201 taattcttct tgaatcattt cttcaggtaa agtgtttatg ttggatattt ttagatgttt
    25261 aaattgatca atcgaatagc catagaattc aactgccgcc gagtttgcat caacgattgt
    25321 cccatctgtt gggtctatga taagcttaat cgcttgtgtt ttctcaaaca tggagtggta
    25381 aagctcttca ctttctaaaa gagcttgctc aaattttttt tcttctgtta tatcttgtgt
    25441 gattgctaat tggattgtag tattagtgct ttgcaactga aggggaactg cattcgtttc
    25501 aaaccattta attctccctt ttgctgttat gattttaaat tgtagactgc ctttttttcc
    25561 atcacagata gactcattga acttcttgaa agtttctcta tgatcaggat gtatcagttg
    25621 ataaacattt ttacctataa catcttttgc atttttaaca tctatcatcg ataagccaac
    25681 agaatttatg ttgactaacg ttccatctcg atctactaat tttatacagc ttggagttgc
    25741 atctacgatt gctctgaggt gagcttcact ttgtttgaga gcaatttcga tgttcattct
    25801 atctgtcata tcttggacaa tccctcgata cgcaatttga atgcttgatt catctaaaac
    25861 aggttcgatt cttgtattga tatattttat tttattattg gggaaatgaa ttttgtgatc
    25921 aaaaaaataa ttcttattga ttaagaaatt ttcactattt gtaaacaaat cagaattctt
    25981 taaatgaata ctttctctga ttttttgaat gatttcttct tggttaatat cttcactgac
    26041 ttcgaaaatg gaataaaatt gttttgaaca atgaacttct cttgttttta gattgatttg
    26101 ccaacttcca agttttgcaa ttctttgtgc ttcttgaaga gagttctcac taatttcaat
    26161 tcttctttgt gcttcatatt cttttgtaat atctttaaat acaaggacaa cacctgttat
    26221 attttcatct ccatctctaa tcggcgcgcc tgaatcggaa attttatact ctcgtccatt
    26281 tctaccgatt agaattttat gttctgctaa tgtaacactt ttttgtgagg ataatacaag
    26341 ttcaacggga ttcgttacga tctttcgagt tttaggatct atgatctgaa aaacttcatg
    26401 cagctcacgc ccctccgctt caacaaaatt ccacccagtg agattttcag caactgcatt
    26461 catttgaata atatttccaa agtgatctgt tgcaattact gcatctccta tcgattggat
    26521 aatcgcagtt attctttttt tttgtttctc tgtttctttt ctggcttcat agagtttgaa
    26581 agccattttt atggaggcat caattacatt tattccagag ttctttacaa cataaccata
    26641 agatgtaatc gcttccgttt tttgaacaaa ttccttttct actcgtgcag ataaaaatac
    26701 gataggtata tcaaaagttt ttaaaatttc tttagcagtc tgcgttccat caattccaga
    26761 tcctaaatca atatccatta gaattatatc gacttggtct ctgttctcat ttactagatc
    26821 gatagccgct tcacctgttt caacatgaat tacagaatag ccttcttttt caagttgtct
    26881 tgtttccatt aaggcaatta tggtttcatc ttctacaagc agaactgttt gttttttatt
    26941 tctactagtc atttgaaatc tcgatttcta atattatagg acgcctaaaa ataaaaacaa
    27001 cttcttggtc aaataaagct agcattttat gaattcctac ctgcaatcat agatataaat
    27061 aaatctgaaa gcaaggagaa ttatgctgaa aactcgtaat aaaataaatt tatttcctag
    27121 tttccactat cgataaacat cttaaaactt aaattcgctc tatttttttt gtgaaacaga
    27181 atcagcaaaa tttttagctg catttataaa ttcagctggc atcattatga ttgtcgttgt
    27241 attattatcg attccaattt cagagagcat ttgcattctt cttagctcta gtgaaaaagg
    27301 actcttctcc atttcttttg cgccttgcgt tagttttata gatgcttcta attccgcctc
    27361 tgctttaatg attctagctc ttttctctct aattgcttcc gcttctcttg ccatagctct
    27421 ttgcattcct tcgggaattt ctacatcttt catttccacc atttcaattt taattcccca
    27481 tggctcagtc gaagcatcta caattttttg taatgttgcg ttaatttgtt ctctttcttt
    27541 taagacttca tctagaatat gttgtccgat aatatttctg agagctgtga ctgaaaattg
    27601 ataaactgct tggttgtagt tagctacttt aatgatggct ttttcaggat cagtaactct
    27661 aaaccataaa accgcattta ctttaattgt gacactgtct ttcgtgattg tttcttgctg
    27721 ctctaattca actgtgctcg tgcgtatatc gactgtttgc tgtctctcta gtattggaat
    27781 tatccagtaa agtccgggtc ctttcacaga ttgaaatcta cctaagcgaa atacgatagc
    27841 tcgttggtat tcttgtgcga tgcgaagtcc aagtagaaga aagatgaata tagaaataac
    27901 aaaaggtata aattgttcca taaaaaaact ccatagattt tgcgtttaat gtttaatagc
    27961 cactcgatct ccaaaaactt tataagctag attttttaca gattcaaaaa aatgaatttt
    28021 atataaaaaa ggatttagcc aacccactgt aatacaataa tacttatcga atggttcagt
    28081 atgatgaatt tgatgatgct ttggagaaag gatcaaatta ttcctttgta gaaaagaaat
    28141 aattttgcct gggttgtctt catgtgccca cttgtgaatt tggtttgtaa taaaaacagc
    28201 aatcattagg aaactaaaaa agctataaaa agaataggta aataaattag ctttgatatc
    28261 tacaaagtaa taaacagcaa gcaatgccgg aatggataca atacagttat ttccgcttgt
    28321 ttctacaaag tcgtgtttag tcatcccttt tggatcaaca tggtgttctc tgaatggttt
    28381 tataaaagac tgacccagaa caggagtatt ttctgttcca aaagtatctc ctagaaaatg
    28441 aacaaagcct gaaataaaat cagcaccaat gtatccaaga aagaatggaa aaattgaaag
    28501 tggaatcacc agataactta gattatccgc aagacgaatg gtttcttgga tgatctgaat
    28561 tacaagcatg gagcagtata ctatgaaaat gaaaatacta gcaatttcta agtgtctatg
    28621 tttatattcg aacattgaat ttctccttat tcttttttgg gaatcgctga tcccgttaaa
    28681 taaattatat ctgcgaactt cttaaaatga cttagttcaa tattttccgt ttctctgttc
    28741 tgaaacattg aaatagttag tattttaaca ccgagttgct gcaatctgtg gctaacaccc
    28801 atatactcat cactatgaaa tcttccattt atatgaatga ttttagtctt ttttttatct
    28861 aatactttta aaatagaatc agccattgtt gcatcccaaa gattttgggc gagtatcata
    28921 ttctttaagc caattgtatc ttttccgtga cttgcgagag aaccaaaaat tttatgttca
    28981 taattttcct gtctatattt ttcaattgta tagattggag caaagaactt tctagaaagc
    29041 gaaggaaatt tatacatttc ctccaatcca tttcttgcaa gtattcttac atagcgccta
    29101 ggtgcatttg ctgctaggat tgaaatttta ttttctctcg catactggac aattggttta
    29161 tagtcatcgt aattattcca gagttttaag tctgtttgaa atattttatc atcaatcaaa
    29221 ccagcaagat attcatttaa aatgatttgt tggtccctct caaacatttc catggagata
    29281 acaaacgtaa accgctccga gagatatcga atgagtttta atttttcttc atgccctttt
    29341 ttatcatcat gctcttctcc aatgatcaat acatccactg gatttaattt ttcagcaata
    29401 ctttcataac ttatctcttt tccggctgct gaatcaaata tcttgggata gccttcttgt
    29461 ccaaataggc taatggtgaa taggagaatt gataaaaaga aaatctgctt tctcatattc
    29521 taaattcgct cagaatttgt ggagatatta tagatagttt ttctaaaaat ttcctgtgga
    29581 ttgttgcgtt cgttgcgact aagtttggca taaagataga gaagttattt ccatcgtaag
    29641 ttgtaattct tccccctgct tcttctacta tgatagatgc ggctgccata tcccaaggtt
    29701 tcagattctc ttcccagtat gcatcaaatc ttccctctgc gacccagcaa agatctaaac
    29761 ttgctacacc ggttcgtcta acgtctctta ctgaaagtag aacagatttt aagttttgta
    29821 ttacactttc cattctagct tttttttcat aaggaaatcc tgtagaagca agtgaattaa
    29881 ttaattcatt ggttttggat acataaatct gtttttcatt tttgaaagcc ccataacctc
    29941 taatcgcata gtaaacatct gacatagctg ggaaactaac caatcccatt tccatatgac
    30001 cgttttcaat gttttctaag cctatgcaga ctccatagag aggaagtcgg tgtgcaaagt
    30061 tagtcgttcc atctacagga tcaataatcc atctatattg attctttcct ttaatggaag
    30121 aaccttcctc tcctaaaata tggtcatcag gaaagttctt tagaatttcc tgaacgagca
    30181 ttttttctga gcctttatcc gcttctgtta ccaagtcaat cggtcctttc aattgaattt
    30241 cgaaggaatc tttgttttgg gtatgaatta aaaatgcctg aattttaggc aaaaaggcat
    30301 atatctgctc aaatctttta cgaatatctt ctttcattgt atgattttac tctcatggct
    30361 ggataaatac aaagttccgt atgaatttat ttttgtctat caatagaaga aagactatat
    30421 aaatgaggtt agcttaaaat tctggttata tattgttaaa tcttttgatt caatggagat
    30481 aaaagaatgg aaaagcaaat tatggatatt ctaaatgttg ggattggact cctacaagct
    30541 ggaaaagaag gcttagaaag agctaaaacc gaattagaga agacctatac agaacttagc
    30601 acaaaaggtg ctcaagacaa ttctgagggt gctgttcaaa ttcgccaaag tttagataag
    30661 attattcatg atattaaaga atttactagt gttgctggca aaaactatga agacactcgt
    30721 tctaagatca ttgataacta caacaaaatc acggaagaaa ttcaaaataa aatgccagag
    30781 ggcaaagtag aggctgtaaa agctaagatt agtgaagtag cagaaaatat taagaaggca
    30841 aaaaaagcgt aagataattt ttcttccaat ataatctttt ggattctcag attgaataaa
    30901 gactttttta ctttattcga tctagaatcc aatatcacac ttctgccaat ctcaattggt
    30961 taatatcaag ttacaacact tcgattttta atgctaacta agaacatgaa tttaatagtt
    31021 ccaaaatata aaccaagact tgagatagat tcgtttttca atgcaagccg gatgaattta
    31081 aatccaattt ataaaggtaa aaataaatat gaataaaaga aaacttttag taactactgc
    31141 acttccctat gcaaatggac ccattcattt agggcatatg cttgaagggg ttcagaccga
    31201 tatctgggtg cgttatcaga aattatctgg taatgattgt tatttctttt gtgcggatga
    31261 tacgcatgga actcctgtta tgctagctgc aaaaaaagaa aacatttcac cggaagcgtt
    31321 agttgaacga attcatagtg agcattatcg tgatttaaaa ggcttttata ttgagtatga
    31381 taattattac tcaacaaact ctcctgaaaa ccgttatttt tcagaagaaa tttataataa
    31441 tctcaaaaag aattcacaca tagcagaaag agaaattgaa cagtcttatt gtgatcatga
    31501 taaaatgttc ttaccagata ggtttattaa aggaacttgt cctaaatgta aaactaagga
    31561 tcaatatgga gatggctgtg aagtttgcgg ctcgaattat tctccaaagg atttaattga
    31621 ctctcattgt gcgatatgcg gtaatactcc tggtcttaaa aaatcaaagc atttattttt
    31681 taaattacaa gactttcaaa aagaattatc tgattggatt gaaaatcctg agcatgtaaa
    31741 tgctggagtt cgaaataaat tgaatgagtg gttttctcag ggactacaag aatgggatat
    31801 ctcgcgtgac ggtccatact ttggattcga aattcctgga gaaactaata agtattttta
    31861 tgtttggtta gatgctccaa tcggatatat ggcatcttct aagaattttt tcaaagacaa
    31921 tgcccttttt gatgagtttt ggaaatcggg tgatggggag attattcatt ttataggtaa
    31981 agatattctt tattttcata ccttattttg gcctgcaatg ttattaggtg gcggatacaa
    32041 aacacctaat cgagtgaatg tgcatggctt tataacagta aatggagaaa aaatgtctaa
    32101 atcacgtggg acatttatta aagcctctac attcttaaag catttagacc ctgaacattt
    32161 ccgattcttc atggcaggga agttaaatga tacgttagac gatttagatc ttagtttttc
    32221 agattatatg tctaaagtaa attctgactt ggtaggaaat ttcatcaata ttttatctag
    32281 aacaggaacg tctattttgg ataaactcga taggcgaatt ggcaatttaa attcagaggg
    32341 gaaagctctt atatcggaat tgattcaaag taaatcagat atcttaaaga attatgaaga
    32401 caaaagctat tctaaggtaa tgaaggaaat tgcccgttta ggcgatttgg tgaataaata
    32461 tgtaaatgat aaggctccat ggaatcttgt taaaacagat aaagaggcag cgagagaagt
    32521 tgtgacagca gccattaatt gtgcaagaat tctcgcaatt tatttacaac ctgtcatccc
    32581 aaagatttgt gaaaaactat ttaagatact tgatcttcca gcacagattt ctttttctga
    32641 gttggaaagt gtattagaaa atcgtaatat aaatgcgtat gagattctat ctcagagagt
    32701 agatgaaaag gcagtgaata gtatgttgga agaaaataaa gaagcacaaa aagaaagtaa
    32761 aactgaaact attaatacaa aaaaaacaga aaccaattca aattctgatt caaaattagg
    32821 tctaatcagc atagacgatt tggcaaaagt agaattgaga gttggtctga ttgaagaagc
    32881 aaatcatgtt actggcgcgg ataaactttt aaatgtcaaa gtcaacttag gagaaaaagg
    32941 aattaagaat gtatttgctg gcataaaatc tgcatataaa ccagaagaac tagttggaat
    33001 gaaagtagtt gtagtagcaa acttagaacc tagaaaaatg aaatttggtc tatcagaagc
    33061 aatgctattg gcaacaggaa aggatgaatc tctttctttg attgttccac atagaaatgc
    33121 aaaaccaggg gatattctga agtagccgtc atgtcactaa taaaaaatat ttacatagaa
    33181 aataattcac atcacaggct tggcataact ccaaaggaaa tcaaagcgag tattaaaaaa
    33241 gttgtagatc atatgaccaa tttaaaagga tacgagcttt ctcttttagt tacaagtgat
    33301 gaagaaatcc agaaggtaaa tttagccaaa agaggaaagg atacaccgac tgacgtatta
    33361 tcttttccta ttgtggaaaa agatttacca gaaggatata aaatattagg agaagtgatt
    33421 atttctattg atactatgcg taagcaggct agggaaattg gtcatagcga caaagaagag
    33481 ttttatagat tgcttgttca tggcattctt catttgcttg gctatgacca cgagaagagt
    33541 gcatacgaag aaaaaagaat gcaagaaaaa gaagatgaat gtttaaaact tatattaggt
    33601 taaatttcga ttaatagcaa ggtcatatcg tctttcacaa cattgccaga gtaattaata
    33661 gaaaaattaa aaattgattt tagaaaatca tttccttgta gatggcacat gtcttttact
    33721 ttttctagaa actgctcact tccataaaat tcgttattta aattataaat ttcatacaat
    33781 ccatcagaga aaagaaaaat cttatcccct ttttctaact gaatggaatt ggatgcagta
    33841 ataaattctc tcattaaaat caaaggagtc cctttccctt ccaattcaat caattgattg
    33901 tttcgaatta agattgcagg atggtgaccc gcataggcgt attgtaaaat tttattttta
    33961 gtatccaatc gtaaatagat tcctgtgata tagtgacctt ttacataact aaaaagggtt
    34021 tgatgtaaat atttaaattc ttcttcaatt tggttattcc gatccatcgt ttgaaaacaa
    34081 aggactgcca tacaagaaac cattgcagaa gatattccat gcccagagac atctccgaag
    34141 agtaaatcaa ttgtatcttc tttttctaga aatgagaaaa agtctcctcc tacttcattc
    34201 attggtttgt agtaattaaa cattttacaa gaacgagaac tgataaaatt ttttggaatc
    34261 agggtttctt gaatattgcg agcctttcgt aaatcttttt cgagggaagc aagtgcttct
    34321 gagagtttct ctttggaatc tgaaatttct tttttttggg attctatttg taaaatatat
    34381 tctcgattcg aaagcgcgga acgaattctt gcaagtaata cttttggaat cactggttta
    34441 atgataaaat cggttgcacc tgaatctaga cagttttcaa ttatatctgg gctctcctct
    34501 gacgttagca ttagaatagg aatatgagca aataactcga cagaacgaat tttacttaga
    34561 atctctaccc catttacacc gtatagataa taatctaata cgattaaatc tatcttttct
    34621 ctttcaatga tttcgagaat attttcagaa tgggaagtgt tgaaaaccct gtaaccttta
    34681 tcagatagaa tgagttcaag cgataaaagg acgagttcat cgtcatcaac gagtaatatc
    34741 gaaaaatcgc tttcattcat aggataaatt taccataacc aagttaaaat ctttgactct
    34801 tgattctatt catttttaac caaaaatgga aacaaatttt ttttgtgata ctacgtaatt
    34861 gttgaaacat tttattaaaa aagccgcaca ttttttgaaa ttatatctcc tttattaata
    34921 aaagaccaat aaaaggaagt taaatatgtg cgcaaaatat aaatcatttt actattttat
    34981 atgcagttta cttttgtttt cgtctatctc tataggaagc gatgagaata agacgttaaa
    35041 tgataacagt attctagcta gaaagattaa tttagattca gaaagtgatt gggaatattc
    35101 tctcgatgat ataacagatc caacccaatt tgaaagtaat cttaattcta ctcattggaa
    35161 gaaatacaaa gttccttcta atttaaattc aatagaaata aactctaaac aaagaatatg
    35221 gatcaaaaga aaaattatcc tacctgagaa tttatcgaat aagcatttat cgatacgatt
    35281 gggaagtatt tccgatagag atagaactta tttcaatgga gttttgatag ggtctactgg
    35341 atgttttgga tgcgatagtc ctcaagccta tgataaggtg cgaatttatg atattccaaa
    35401 taacctaacc caaaaggaaa atattctttt aattcaagta gagagattct ttccagagga
    35461 gattggtatt aatcaggatt atacagcgat tggaattaca tcttatttag agtatgatcg
    35521 atacaaggaa gatttattta aacttatact cctttcaatt tatggaacag taggttgtta
    35581 ttttttattt ctatatataa gaaggcggca agaagttgaa aatttatatt tttctagttt
    35641 tgtttttaca gtcatagtat atcagttttt aagaactcaa attaaatacc aactcaatct
    35701 cgactttgtt attttgaaaa agattgaata tatgttcttt gcaagcttga tacctctttt
    35761 ttcaaatttt ataagagtct attttaaaat aaaaataagt agatttgtat atctcttaaa
    35821 tctattcagt ttgttttcta tcggattcta tttattaagt tccaatttgg ttttatttga
    35881 tttatataat aaaagactta ctcagccgat aggatttctt tatattgggt ttttattcta
    35941 ttatttatta aatgcattta ggagcaagaa tcgagatgca gtttattttc tgagcggagt
    36001 ggtgctaatt tttatttctg tcgtgactga tatattaagc gataggggat actttcagtt
    36061 tcccagaact tttggatata gctttatgtt ttttgtcctg agtatcgcga ttattttggc
    36121 aaataaattt gttcgcctgc attcagaagt ggaagaatta aatacatccc tagagcaaaa
    36181 agtaaaagac agaacaaaag aattaaatga atctatggag gaaataaaac aaataaaaac
    36241 acagcaggat ggagattact ttttaacatc tcttttactt accccactca catccaatcg
    36301 aaatcaaagt gagtatgtga aaactcaatt tttttcaaaa cagaaaaagt cttttcattt
    36361 taaaaacaaa acctatgaaa ttggaggaga tataagtatc tctgcaaata taacgttaaa
    36421 cgataaatta tatacagttt ttattaatgg agatgctatg ggcaaatcca ttcagggagc
    36481 aggtggtgca cttgtgatgg gtgttgtttt taatactgta ttatctagat cacaagtagg
    36541 tagttttaaa actaaaactc cggaaaaatg gctaaaagat gcttttttag aattacaaag
    36601 aatttttgaa tcctttgatg ggactatgtt tttgtcctgt gcgatgggac ttattgatga
    36661 agaggcagga tttttatact acctaaacgc agaacatcca tggacgattc tttacagaga
    36721 tggaaaagca aggtttattg aagaaaattt tacaactaga aaattaggta tttttgaaaa
    36781 tgataaattg ttttcagtga agacaatgaa actactaccc ggagatgttt taattctagg
    36841 ttcagatgga cgagacgata tccaaattgg aataaaggac gatattcgaa ttataaacga
    36901 agatgaaaat ctttttttag atattgttca aaagggaaat gggaagttag cggatattgt
    36961 agagctttta cataaaacag gtgagctaac agatgactgt agcctggtaa gagttgaata
    37021 tttgggaatg ccagtgaatg ggaaggtaaa ctttactgaa atattatcta gattatttaa
    37081 atctcattat gatatgagaa atgaaaatat ggaggataat gtgcatttgc ttcgaagtgt
    37141 ctataaactt catccatcca atgaagtagt aaatcggcaa ctgagttttc attattttaa
    37201 aaaaagggat tttcacacag ccattaagca tatcaatcaa tatacttata aaaagcctga
    37261 gtctacagat atgttctatc ttgcttccgt tttaaataaa aaatgtggta attatgaaaa
    37321 ggctatagac attggagaac agattctgct aagagatcca aaaaatttta gaaacttaaa
    37381 aaatctaaaa gagctgtatc atattacgca aaaaatggat cgagtggatg aattagagag
    37441 gagaattaaa gaactaaaaa atgaagtatt tagagttaaa agttaatctg ccaaaagaag
    37501 tttcggagga atttacagcg tttttagatt cactctcagt ggaaggatat tatgaaatac
    37561 tatttgattc tacattgcct agacccgcat cgggagaaat tttaagagac gatacaaaca
    37621 tacatgtgta tctaggtgag acggatttag aaaaggaaat gaaaatttat atttatctca
    37681 aagcagttgc aggagataaa tcttttgttg aatctagaat cgttgagaca aaggaatttg
    37741 aagaagcata caaagaattc tacaaaccat ttcaagtagg ggaaaatttc tggattgttc
    37801 ctacttggga aaaggaaaca gaatttacta aaaatttagt tcagaggcaa aataatatta
    37861 tcctctttat gaatccaggt ctcgcttttg gaactggtca tcacgaaacc actaaactta
    37921 tgatcgcacg cattgataaa attgttcaag aagggatgtc tgttttagat ttaggaacag
    37981 gttctggaat tctttccatt gcagcagcct ataaaaaagc aaaggaaatt ttagcaattg
    38041 atattgatcc taatgctgtt cgtgcaacag agtttaattg gaaggaaaat agttttccag
    38101 atcccgttca atttacagtt caagaaggtg gttttgatca tccttctatt ggaaataaac
    38161 actttgattt attgcttggt aatattacct atgcagtcat atctcaaaat attgaatgga
    38221 ttcaaaagat gaacatcaaa cattttcttt ttagtggttt aatcattgaa aggcgagagg
    38281 attctattcg gttatttcag gaaaagttag gcggaagact tgtctatgaa gaacaattaa
    38341 atgattggat tatccttgaa tgggagagat aaatctccat ataacagagc caattgattg
    38401 tcacctttgg aagattcgtt ttccgagtgt tgaagattgg cctctggatt taaaaacatt
    38461 agttgttttt gaggaggatg ttcattttga aagaagttta aagcagtgtg actgtggaca
    38521 attgtatttt tatgaaatga tagaatttat cgactttgag cgaggagagg atccaatcta
    38581 tcgaactcta attccaattg cggatgagca aacagctgtt agcctaaata gtaaatctaa
    38641 gtttgagctc atcaatttct tatcccttag atatgattgg ccaaataatg agaagaatgc
    38701 aagagtatat tggcataacc gttctgagac cttctaataa aactttaatt tctttaggct
    38761 ctctataata aatctttgat ataaatcaaa aataaacagt agtcgtcttg atccgttttg
    38821 tggcaatagt caaatagaaa aggattcttc atgaaatcaa ttatatcatt ttttgtttac
    38881 agacctctta tctcaaattt aattattgtt tttcttttat tggctggctc gctgagttta
    38941 ctctttatta aacgcgaatc ttttccaaac gtaaatttat accaaatcaa ggtaacaacg
    39001 attttccccg gagcatcgcc tgctgacatt gaacaaaagg taacaatccc aattgaagaa
    39061 aaattaagag aggtgagtgg atttgattct gttcgttcta tttcacgcaa ttcagaatct
    39121 gatattaata ttaagataga tatcgataat aaaaatccag acaaagttgt agatgatatt
    39181 cgaagggcag tggataaggt aacagatctt cctgttcaaa taagggaaaa gccacttgta
    39241 atagaacaaa agagtagcga ttttccagtc ctagagattt cggtttatgg tggtaaggat
    39301 gaaatcgaac ttcaggcaaa tgctaaattt attcaagatg aactccgaaa agttgaaggc
    39361 gttggtcgcg tagatgcatt tgcaaaaaga aaaaaggagt ggcaagtcct tgttaatccc
    39421 tcctctaaac aaaaatacaa tgtcggattt tatgatgtta ttctggcaat agcagaaaga
    39481 aacgttagta tccctggagg atctataaac gcagaaacaa caaaagatat tcgggtgaca
    39541 ggagaatttc aagagattgg agaattggga aaaattccag ttcgtgcgaa cgaatccggc
    39601 aatcaggtgt ttatttctca aatcgcaagg ttaaaagaca catatgaaaa agaaagaaca
    39661 atagctcgca caaatggaca taacgctgtt aacttactaa ttataaagaa agagcaagca
    39721 gatattattc gcacaataga aaaattaaat attaaactac aaagtattga aaaacaaatt
    39781 cctgaaacga ttcacatttt agaattaaat aatgagggaa agaagactat caatcgattg
    39841 aatgttgtga tatccaattc agttcaggga ttaattctaa tttttatcgt cttatttgtt
    39901 ttctttagtt tgcgtgattc aattcttact agtttatctt taccactttc cttacttggc
    39961 tctttacttt ttatgcccat attagacata tctttcaatc ttatttcaat gcttggtata
    40021 attatttcgc ttggaatgct tgtggataat agtattgtta tttctgaaaa tatttataac
    40081 tatcgtatga aagggatggc tcccaaagat gctgcgatag tcggaacctc ggaattggtc
    40141 gtgccgattg tgggatctta tttaacaacg attgcagctt ttattccgat gctctcaatg
    40201 agtgggatta tggggaagtt tgttgttcaa attccgctta tggtaataat cactttgaca
    40261 atgagtttat ttgaatcctt tttccttttg ccagttagaa ttgctaagta tggtaagata
    40321 aaaacagaag taagagaatc tttttttgaa aaattacgtg gaaagttgga tttatttttt
    40381 gaaacaatga ttttgaaatt tgaatcatta actgaaaagt tggtgagaag aaaatatata
    40441 tcactatcaa tcatagcatc tattttttta agtgcgttag ttactattag tttgatgaaa
    40501 tttaacttat tcccaaaaga agggatcgaa caggtaatta ttaaagcgga atttcctcct
    40561 tattttaccg caagagaaac gctagagaag ttgcaatatc tagagcctat tttggataga
    40621 attccaaaga cggagattat tgggtatact gtaaaagtag gaattcagca gactgatagt
    40681 ggtgatccac ttactagagt aggggaacat ctaggaatgg ttcatcttta ttttattcca
    40741 gaaaatgaaa gaaagcggac aacgtctgag attatagggg aattagaggc agaaattaaa
    40801 aaaatcccta atgccgaaaa tgtatttatc gaagagttga tcaatggacc tcctattgga
    40861 gctgcggtta ctgttgcagt tgagggaaaa gattataaaa agttaaaaga aatttccaat
    40921 gaaatcaagg actttttaca atctatgaat ggagttaaaa atatagcaga tgattacaaa
    40981 aaaggaagag aagaaattgt tttaacatta gattccgaaa gggcagctcg tgctgggatc
    41041 agtgttgcaa ctaccgctaa ttttgtgaga agtgcatttg aaggaaatga ggcatctaga
    41101 tttcgtctag gaaaagatga agttagaatt aaagtccaaa atgatgcgca gtataggaca
    41161 tctcaagagg atataaaacg ggtaaatatt atcaaccgct atggtttgtt tactcctctt
    41221 gctagttttg tgaacattaa aaatgagcaa gggatagagg cacttcttca ttttgattat
    41281 gagaaggcaa ttacgattac ggctgacgtg gatgaaaaga taatcacatc tggaatggtg
    41341 aacggaagtc tcgcaaagca atttggagat ttagagaaaa agtatccagg ttattctatg
    41401 aaatttagag gagagcagga aagcacgaat aaatcaatgg cttctcttgc gagcgcgggt
    41461 atcattgcat tttttggtat cttcgcaata cttgcgatca tattcaataa cacagtaaaa
    41521 ccatttttta ttatcatgtc tataccactg ggattagtcg gtgttatatt tggatttcta
    41581 atttcaaaca aatctcttag ttttcttgct atgatcggaa tcattggtct tgcaggtgtc
    41641 attgtaaata catcgattgt gcttgtggat tgtattgatg aattacaaaa gaagggaatg
    41701 gataaatatc agtctctagt gaaagctgcc tctagtcggt ttcgcccaat tattctaaca
    41761 acaatcacta caatggcggg tctgattcct actgcctatg gaattggggg gacggatgca
    41821 atgttagttc caatgactct tgcgctcgca tggggacttg gctttggaac gataggcgct
    41881 ttatttttta ttccaactat tttttctatt gggtatgatt ttaaatcttt tctacaaaag
    41941 caattcagga tctataaagg tcatgaaaag acatgagtag taaaatcgaa tacgaaataa
    42001 ggcaagctca attggaggat ataaatgcaa tcatctctct catgattgat ttttataaag
    42061 aagctggatt tcatttaaat attgaatcaa ccgagaaagc tcttcagtat ttaatatcgc
    42121 atcctgaaaa tggatttata ttcatagcat ttagggacag gaagccactt ggacatatag
    42181 ttctatctaa acgatttgca atggaatatg aagattttat tgggtatgtg gatgatttgt
    42241 ttgttcaaaa agaatttcgt cgtgctagga taggaaatgc tctattagat aagttatgcg
    42301 aaagagcaaa agagttaaat ttgaaatcat tgcaggtaga agtaggggaa aataatttac
    42361 ctgctataaa attatatgag aagattggac ttagagctta tcaagacggt cgtgttttac
    42421 tctctaaaaa tctaagtaga aataactgaa ttatttttaa ttgtattttt attttatcga
    42481 attgaatttg actatcagac taatgaatat gataaataat agaaaaatga ataatcttct
    42541 cctcttaatt gtttgtattt tattttctat ggcttcttgc gcagaaaaga aagagaaaaa
    42601 taaagaaatc aagctgttct cacaaatagg tttcttaagt tttgcaacta caaactatcg
    42661 ttgtaatatt actccatcga gtaattatgc atactttaat tcaggcacag ctccaactgg
    42721 ttctagcgta tcggttcgta gaattttttc tgcacttaga agtgcaggca ctgtagaaat
    42781 ttattatcct gagacagtta cgaaaaattt acccgtgtta gttatatacc aaggtggaaa
    42841 tgtaaatgga tctttttatt ccaattatgc ggctagatta gcggcttctg attatattgt
    42901 ctatcttgcg aatcgttgca caacttttat tttacaatat ttcatctatc cggatgcatc
    42961 tttagcgagt gaggtttatg atcttgcaag aacaaatacc actgattcta gttctcctct
    43021 ttttggtaga atagatacag aacgaattgg tgtgcttggg cattctcttg gtggagtcgt
    43081 tggtcttttt tcgatcaatg gtatatgtga ttttcctttc tgtgcaagac ctacatctcg
    43141 cctaacgcaa ataaaagcag gtcttgtgta tggtagtggt ttgggagaag ggtttgataa
    43201 aaaaagattt tataccgatt caagtggaaa agggattcca attggttata tgcagggatc
    43261 tcttgatact gcaaatacac cggactcatc cacaaagagt tacaccaact ctatttcccc
    43321 taaatattta ttctcgattg atggtttgaa tcactatgga attacagatg taaataatcc
    43381 gttcggtgca aagtcagaaa caaatctaag caatctctct caatcacaag ctattgatag
    43441 gatttcaaat gttagtttac ttttcttaaa tgcctatgta aaaaatgatt caacttctct
    43501 ttctacgatc aattctggta atttaggaat tactggtgtg acaactaccg catctccata
    43561 aaagttttaa gatatgaaca agaaaatatt agtcactgga gcaaataagg gaataggttt
    43621 agaaattaca aaacagctga ctgccatggg gcataccgta attatgactg gtcggagtct
    43681 tagtaaatta acagaggcaa agaatagttt gccgttagat gcaaagacag atttctatga
    43741 gatggatgtt tcctctggta aaagtatttt agatgcatac gaaaagatca aagatgttta
    43801 tccatacttg gatgtaatca ttaacaatgc aggcattcta attgatgagg ataagtcatt
    43861 atttgactca actgaagatg attttttaaa aactattcag acaaattcaa ttggaccttt
    43921 tttggtgata aaaagctttt ctaatctttt aaaaaaaggt gggactatta taaatatttc
    43981 aagtggagga gggcagataa cgcatggagt ttccacttgg tctccacttt actgtctgag
    44041 taaaacttta ttaaatgcaa tcactctaca gtttgccaaa gcactagagt ctaaagaaat
    44101 cacggtcaat gctgtttgtc ctggttgggt caaaacggat atgggagggc agagtgcctc
    44161 tcgctcagtt gaaaaagggg cagaaacacc tgtctggctt gcaactagca aagtcagttt
    44221 tactggaaaa tttttacggg ataaaaagga aattgattgg tagttttaaa aaaacgattc
    44281 agtgagtcaa tttgacagtt gtgctagaat aaaaaacaat ttgattttct ctttcctaca
    44341 cttcgtaact ttttactaaa cactacaaaa aaatctatgt gaaaaagctt tcttttgaaa
    44401 taaatgaaat atcaaaggag ttattagaat gatttttcag aattttaaaa tgtatcgtat
    44461 aatagcaatt atccgttcat tcattttttt attagtatta ctgactctat cgcttggagc
    44521 agaatccgtt gaggaagtga aaccttggct tggtgttgca attgaaactg ctcccaatgg
    44581 agtgagagtc agagaagcga tagtaggaac tcctgccgag aaagcgggac tgaaaaaagg
    44641 agatgtgatt acgaaaatag attctatcca aatgaaagag tcagagcaaa tgataaaata
    44701 cattcgttca aaaggtgtag gaaatgaagt gaaagttgaa tttgagagag atacaaaaaa
    44761 gaaaaaatta gcaatcgtgc tagaagcaaa gcctgacgaa tttgatgtag ttaaaaaaca
    44821 attacttgga aaagaggttg ttgattttaa attaactagt ttagatggaa agaaatcata
    44881 taccaaaaaa gatttagaaa ataaaattac aatttttgag ttttgggcta cttggtgtat
    44941 gccgtgcaga gcttcacata caaggctttc tgagtttgca gaacaaaatc catcgattca
    45001 ggtattagct atttctacag agtccaaaga cttaattcaa aattatttaa aagaaaaaaa
    45061 tcataaattt actaccgttc aagatgagaa aggagatgtt tcaaaattct ttatggtatc
    45121 agctattcct actaccgcac tcattgataa gaatggtaaa atcaaatttt tatcactagg
    45181 tggcggaatc tacttagagg aaactttaaa gaatgctctt tcccttagca aagaaaaata
    45241 aagtatagca agtaagaata tgcgtagatc tgaatttaat atgaaagaga aggaagagat
    45301 agattccttt cttctaaaaa tgagttatgg gtttttagct actcatagct ctgatgaatt
    45361 tccaaacctt attcctataa actatgtatg gtttcgtgac aaattttatt ttcatggggc
    45421 aaggactggg agaaagatga ctgaaatgaa agcaaatgaa aaagtttctt ttgccattgc
    45481 agatgaatat tcaatcatac cttcttattt tacggatcct aagtttgcct gtcccgcaac
    45541 gtcatttttt aaatctgtaa ttgcttatgg tgttttagaa attattgaag atttagatga
    45601 gaaagtctta gcgttaac
//
LOCUS       DAOQOS010000037        45208 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_323_length_45208_cov_6.607313, whole genome shotgun sequence.
ACCESSION   DAOQOS010000037 DAOQOS010000000
VERSION     DAOQOS010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 45208)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 45208)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45208
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_323_length_45208_cov_6.607313"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..407)
                     /locus_tag="PLF66_10010"
     CDS             complement(<1..407)
                     /locus_tag="PLF66_10010"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HMZ64418.1"
                     /translation="MALNDLGKEMQGSLTQAENSAELSRRANQYAEVGANLGLNTENS
                     ISEIQEESKAIIDYAQLISEISDQVGLLSLNASIESARAGELGKGFQIVAKEISKLGE
                     HTNENSELISKKIRQLSHKIKQGYDQIKEVSEQ"
     gene            complement(398..1183)
                     /locus_tag="PLF66_10015"
     CDS             complement(398..1183)
                     /locus_tag="PLF66_10015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HMZ64419.1"
                     /translation="MIVVQLQWILGAITTATVYYSFVPVSLYGVITFVLLIVILAPVM
                     YMIHSTQADLFISDILTLPTIRNIPVDTKNIRAITILHRVSIASFSILFLPVGILISL
                     YYFGSLSDLDDPFRNYLVGLIGFQSLVLSFICANLLSKILSKNTENVKEALAELKNGN
                     LAYRMPLVDSEELGFVLALNFNSLSENIFEVIHHLKATSNKLNLLSQNLELNATHVST
                     EAENQSNYVRELLLVWKNFKQQSPKQNKTQKSRSGYPKIAPWH"
     gene            complement(1187..1480)
                     /locus_tag="PLF66_10020"
     CDS             complement(1187..1480)
                     /locus_tag="PLF66_10020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64420.1"
                     /translation="MEEKIKKQIISLSIRMELTNYVGGVPLAVFFLVLSQKMYYEKFY
                     YIALSFLAAVSITLIIFPYWRAKIFHSLFDPLLVGTKDSELSFHKENLLKFPT"
     gene            complement(1559..1792)
                     /locus_tag="PLF66_10025"
     CDS             complement(1559..1792)
                     /locus_tag="PLF66_10025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64421.1"
                     /translation="MVFFIGDALSSLAFFYYKNVSSIYLSSGCRLICIQLLPFYLTSE
                     WLKAKKDCESFRKRYFELISILIVRYYRGLVPI"
     gene            1895..2536
                     /locus_tag="PLF66_10030"
     CDS             1895..2536
                     /locus_tag="PLF66_10030"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HMZ64422.1"
                     /translation="MDKIIIFDMDGVIVDSEKIYTDMNQKWFTELGFKLPIEVHQKYI
                     GISAKIFWTFLKNEFKLKEPIEYYIQKEKELKFNTLSNLHLTPTNNLMNFISYIKSKK
                     YKCALASSSLKMNINLILTKLNLLNHFDFVISGEEVTSGKPNPEIFLRVKDFFQANVN
                     QCIVIEDSMNGVTAAKAADMFCIGYFNPNSGNQDLSKADLIIDDFNHPMLYTL"
     gene            complement(2711..3013)
                     /locus_tag="PLF66_10035"
     CDS             complement(2711..3013)
                     /locus_tag="PLF66_10035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002976056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HMZ64423.1"
                     /translation="MKNKRIPTPRVSDILRVEFLEPLKITPYRLAKDLHVSTSTILEI
                     LHNKRRITIDMSLRLAKYFGMSDKFWINLQNDLEIRQQKEKLRDKLENIQTLKKTA"
     gene            complement(3026..3307)
                     /locus_tag="PLF66_10040"
     CDS             complement(3026..3307)
                     /locus_tag="PLF66_10040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010570611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HMZ64424.1"
                     /translation="MIKSFGDKETEKIFSQEFSKKFPPEIQSRALVKLLLLENAQTEN
                     DLMVPPSNNFEHLKGNRKNFCSIRINKQWRITFQFQEGHCYNVSIADYH"
     gene            complement(3495..5555)
                     /locus_tag="PLF66_10045"
     CDS             complement(3495..5555)
                     /locus_tag="PLF66_10045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008594530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HMZ64425.1"
                     /translation="MYFNLRRYLLIGFLSIGQLIAQDIKRSETVEVVGTSTQNDSSKT
                     KSVKADPTGFTEIIDAEKFKNKYTSLTDILEREAGVRIRRFGGLGSYSTLSIRGSNAN
                     QVRIYIDGVPLNNSQGGEINLADLSFDNLEKIEIYKSGASPGFSGSAIGGTVNLVTSK
                     SAKKPSSRFIVSGGSLNTFKLSASHNATIHNVRYSLFAQQEKSDQNFTFRNNNGTFLN
                     SFDDFDDKRKNAQFNRYNFTGNASFDIKQTTVTILNDFNYRKNGIPGPGSNQTKKVER
                     EYLRNTTSLGSMSKSFLFSNLNLDTRLFYTGARDHLFDPLSEFSSGTPNSKAEIQQYG
                     LHLLPTLYLLNSNQILRFLFAFERETFLRDRRNKLDQVLDKSTRKFRHHTTWQIQDEI
                     RLLDKRLTITPAIQQELYIDRFNSQDSLILNTNTEPNKKTTQYTNYRLGLLGIIHKSK
                     ENTFSFKSNIARERRIPDFLELFGERGSVIGNLSLKPERSINTDAGFVFDTQRKNLKY
                     TGSIVYFQKAITDMILFIPNSQFTLRPENIDSASIKGIEFTQNVTLFDKWKLESNYTY
                     QKAINTSDTSYLKGKYLPLRPVNEWHGSISRRFNRAEIGFETNFIGAVFKDRANEYQN
                     YQSARWIYNTFFTYYFKRSTEEDNRELLLTFEVKNITDKRAEDIVGYPLPGRIWYGTL
                     SGRF"
     gene            complement(5662..6090)
                     /locus_tag="PLF66_10050"
     CDS             complement(5662..6090)
                     /locus_tag="PLF66_10050"
                     /inference="COORDINATES: protein motif:HMM:NF015020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIF family HAD-type phosphatase"
                     /protein_id="HMZ64426.1"
                     /translation="MILVFDMDNTLVDELGSSVRPGIIHLLKKLKSEGHELKLWTNSK
                     KDRAKTILSELHLKEYFRDCIFREDYDPDDKGIKKDIRKINGDLLIDDDPLEIQFTES
                     IGRFGFHITPFRKGMKENQKELDDLYLLIRKRNSFFGKLF"
     gene            6139..6342
                     /locus_tag="PLF66_10055"
     CDS             6139..6342
                     /locus_tag="PLF66_10055"
                     /inference="COORDINATES: protein motif:HMM:NF025733.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich CWC family protein"
                     /protein_id="HMZ64427.1"
                     /translation="MKVKTCPACKTEFICNMESIEECQCGKVVLTALQREKIANLYQD
                     CICKNCLVRFNLEEISPNSKTSG"
     gene            complement(6346..6765)
                     /locus_tag="PLF66_10060"
     CDS             complement(6346..6765)
                     /locus_tag="PLF66_10060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004950797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HMZ64428.1"
                     /translation="MKKLYTAVMTASGGRSGNVKSPDGKYEFSLSMPKELRGEGGDGV
                     NPEILFAAGYSACFHSAMKVVATKKKLEIENSSVTASVDIGMISGGGFGLAVTLTVRI
                     PGMEKSIAEELVSAAHQVCPYSNATRNNIEVTLIVEQ"
     gene            complement(6788..7492)
                     /locus_tag="PLF66_10065"
     CDS             complement(6788..7492)
                     /locus_tag="PLF66_10065"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HMZ64429.1"
                     /translation="MNLISIVLVLASVISLSAQESETAVISFLVGKVNIKTQADNKWK
                     PLKKEDKVSSGDSIMTGNGSIATLTYKGSEFKIAANSSIVVNSLYTKENDGSIAVQNG
                     SAWFKVKDLGGKKFIATTPTSTAGVRGTAFGAIYDAKTNSSMHCVCEGKIEVTTATTG
                     AKPKLVEKGNGSSFQKDNADIAMSSYKNLIVKNEAMPEFESKVKESPMLKTCLSCHTP
                     KGWTTSGIMKDDKYGK"
     gene            complement(7568..8293)
                     /locus_tag="PLF66_10070"
     CDS             complement(7568..8293)
                     /locus_tag="PLF66_10070"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HMZ64430.1"
                     /translation="MNLKLNQLVQVTVSIILLSFSSVLAQENDSIAKVASLLGKVSIK
                     SKESSEWKKLKVNDTVQKGDSIMTGNGSMATILYKESEIKLTQNSTIIVNQLFSKNED
                     GIVEVKQGLAWFKLINLGDRKFNSVTPTSSAGVRGTAFASVYDSTANMDLTCVCEGKV
                     EVNPVKQGKPGIVLKGNGVSVKSGDSNLIPISYKGEMAKGESFPGFEKKVKAYPILKN
                     CLSCHTPNGWKAEGFTRDEKYGK"
     gene            complement(8639..9490)
                     /locus_tag="PLF66_10075"
     CDS             complement(8639..9490)
                     /locus_tag="PLF66_10075"
                     /inference="COORDINATES: protein motif:HMM:NF015818.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HMZ64431.1"
                     /translation="MNKIIENGLDSIGISRTGIKIQFLTQSCFPIYLIHTNNHKPLVA
                     KILDQEEMAISEVSSLRFLQSKNCFVPTVHGLYTEKNNSLLFMDYISDNNTRNKKDKL
                     IDSLKTLYSNQSEKWGFGEDNFIGPLPQHNQYYNSFDSFFLESRILPQLKLCLDKKIF
                     DLEFVKKIEKQILKLVHKWELGKKDARLIHGDLWSGNIVFADKAYFIDPSISYSHPEQ
                     DLAMLELFGSPLNTSDLNQFLNELGYDFGFDERVLFWQLYPLLVHVNLFGGSYINQFY
                     KAIDYYN"
     gene            complement(9483..10415)
                     /locus_tag="PLF66_10080"
     CDS             complement(9483..10415)
                     /locus_tag="PLF66_10080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64432.1"
                     /translation="MESIFKDRHLAVIVANCTEEEKRFLKSCIDLQKGKVINWSKDFS
                     FPTLNKNEILWIDGFDRTSLSECVNWSIQIQIIGRLTYEEKRASSISGVTSFWNLDQY
                     SIESMASGYFKKTEEIYPIHALIWTDNKNFNHKLKSIFHSFSVNEITTNNSDYAFSAL
                     REKYFDLLVLDWDTTGIEIIRLLREFKNIRTSTNHFPRVLGIKDFTKMNIFQDLSMGI
                     KDFCPVLFSQAEVLDILVDSLPIGRDELTGTSVTIDKPYLKLHKGKNINQLIFIYPED
                     KDFKREKYILSEEEMNLHIFKRQYEWLKDLVIHE"
     gene            complement(10415..11422)
                     /locus_tag="PLF66_10085"
     CDS             complement(10415..11422)
                     /locus_tag="PLF66_10085"
                     /inference="COORDINATES: protein motif:HMM:NF020039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HMZ64433.1"
                     /translation="MKYSLLLLLALFLNCYANVKTISIDGCKKIVGLPGPEDLAIDRE
                     TGILYISSHERRGTLVEGKLFQINLNSPTESVPTLIETNYPNNFHPHGMSLVKINGKL
                     MLYVISHPSFEPFTNTIEVFQIEKNKVKHVETIQSDTFISLNDLYVMPDGRIFASNDR
                     GTGSMFRSYFDAFFGIKRSQITYFNLKNWTLLDNKVTYGNGILYKKIKDKEYLYRAAT
                     TAEKIYKYELSQNGDTVTLKEVKVINLDSGPDNLEEDEKGDIYIAAHKSMFRFLKHVD
                     KPDYPSPSQIFVLDNNDNLKEIYANDGTEIPASSTGLRYKNRLYISQVFNPYLLSCPL
                     N"
     gene            complement(11465..13222)
                     /locus_tag="PLF66_10090"
     CDS             complement(11465..13222)
                     /locus_tag="PLF66_10090"
                     /EC_number="2.1.1.-"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF023569.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HMZ64434.1"
                     /translation="MSDYIQKESYFTSFDGTKLFFRNWKNSKKQKVNKAMILIHRGHE
                     HSGRLEELVNGLNLEEFSMYSFDARGHGKSPGPRGYAEGFHFMVKDLDSFARFVCSEE
                     KLEMSNIVVVANSVGAVICSTWVHDYAPGIRAMILAAPAFRIKLYVPFAIPGLRLLNA
                     IKNPAFITSYVKSKMLTHDPNSSRSYDNDPMITKEIAVNILLELHDTATRILEDANAI
                     DTPTLILSAGKDWVVKTKPQRVFFERINTPIKKIVYYPDFYHGIFYEKNKSLPIEETR
                     KFIQDCFNTNITNMDYAATNGQVFIQNEYERLKSGPNIFMKFYYSLLRLGIKTLGRLS
                     KGIDIGVENGFDSGLSLDHVYKNKSEGNLLIGKLIDRGYLDAIGWRGIRQRKIHLKEC
                     LKQTIIEILKQKKQIKILDIASGPGRYLQEIALEFPQVEILACDNTDSNIQEGKETAE
                     NQNIKNIRYLKKDAFDTNSYKNLKFEPDIVVVSGLFELFSENRIVSKALEGLSQIVKS
                     NGYCIYTGQPYHPQLEMIANTLGNRDGISWIMRRRTQLELDQIFAKHGFKKINMIIDR
                     WGIFTVSIAKFNKRKVKAN"
     gene            complement(13235..14176)
                     /locus_tag="PLF66_10095"
     CDS             complement(13235..14176)
                     /locus_tag="PLF66_10095"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HMZ64435.1"
                     /translation="MLSVLENPIFISYLKIVSFVLTVAFLILFTIDKVFKKDIQKIWK
                     IYYGWLIMVPIIFLFILIGRTGTIVGINILSLLAYLEFARATKLNKNKSLHTLVILGI
                     LSLGILCYIPDPLLPGVSGWYGLFMALPVYMIGAIMTVPTLLNRSEGMTHNCALAILG
                     FIYFGWMFSHLNFLTNSKFAIGYLFYLLFAVEIGDVSAFVFGKIFGKHKLRSEISPNK
                     TLEGSIGSFFVSMLLPWLLSFSFPLFTTLQFILTGLIVGIGGQLGDLTISFIKRDIGI
                     KDMGTAIPGHGGILDRVDSLIFTAPIFFHMTRYFEMQ"
     gene            complement(14190..14807)
                     /locus_tag="PLF66_10100"
     CDS             complement(14190..14807)
                     /locus_tag="PLF66_10100"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HMZ64436.1"
                     /translation="MSIYQLKSQFQKLLRPVARLLVNLNITANMVTIFACILSIVMGG
                     LLYNFPELKVLWLSLPVFLFLRMALNAIDGIMAKEHNMITPLGTMLNEITDVISDTAI
                     YLSFLSLPQINPYTIIGISILAIISEMTGVVAIQINSNRRYDGPMGKSDRAFFFSILA
                     FVPGFGLTIPYLNLYLSLILMLLIITIINRFKKALEQSKLYQMKK"
     gene            complement(14804..16117)
                     /locus_tag="PLF66_10105"
     CDS             complement(14804..16117)
                     /locus_tag="PLF66_10105"
                     /inference="COORDINATES: protein motif:HMM:NF012982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2/dual specificity phosphatase
                     family protein"
                     /protein_id="HMZ64437.1"
                     /translation="MIQSNSKQENKRPWLLSFIWLAVLVPIFFGSYNFANSYTATRSD
                     VGSLVFSWEYKIPFIPWTIIPYWSIDLLYPISLFICVSRKELNIHALRILTFTVLSVI
                     GFLLWPLRFTFDRPVTDGFFGELFTLLFSFDLPYNQAPSMHIALLWILWMRFRIHLKG
                     ILLLLLNIWCLLIGISVLTTYQHHFIDVITGFFLGVAVTYFIPDIGIKWLRWNLDKRE
                     PALKLASFFFTLSLFLFILSFVLKGQFFWLLWPASSFFLISLGYISFGPNILQKYDNC
                     NTLSACIVFLPYIVCAYLLYFYHRQKLKPFDQILPNLSIGYLPTQLPNDSIVVFDLTA
                     EFCTKEFKNQKYSFPLLDLVPPSEDQISHIVNTLEKEIKERPVFIHCALGLSRSSIIV
                     AAYLLKAGIAKDIDESIQIIQAKRNIKLGKYHINSLLEFQKRIQK"
     gene            complement(16114..16872)
                     /locus_tag="PLF66_10110"
     CDS             complement(16114..16872)
                     /locus_tag="PLF66_10110"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HMZ64438.1"
                     /translation="MSETYKNKNILIVGGSNGLGLALAKAYLNKGAKVGVIARTLGSE
                     GMESLLTNINFKFYKADVQNINEIQTAMKDFSDRIEMIFAVAGFYASRRDQIINLDEI
                     NQMIQVNFFGTYNTFLSGRDVMLAARGGHLVAISSMTALLYYPKATVYSATKRGVLNI
                     CEIFRNGYRDHNIRVTEVIPGYIDTKRLQFLNSQNPSPRPFILSVEEAATKIINELET
                     GKERIIFPWQMYLLVRLISILPLFLQKTILGIKN"
     gene            complement(16850..18013)
                     /locus_tag="PLF66_10115"
     CDS             complement(16850..18013)
                     /locus_tag="PLF66_10115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64439.1"
                     /translation="MFFLKNSKLKIFLFLSLLICIPAFGNPNPKYQRVMIFNGLGFAP
                     MFFLGLYHEAIAQNKKPDLLIATCGGAITGSVINYTNDLEKQKEIILSQDFYDSFSNS
                     IKPGEDSNIFKTILLIGRIIINLPFSKPELVNVYERNLIHVEDTLDYLPQRSFPFQKQ
                     TIPLLIVAGRVNFNKKEVGLNVDEWVNKNKMKPFSEVYISDEETLKTIQNLQVYSFIS
                     KLYPESNVDTEILFRNNIPIVTAMRASTSDAYLYNPTYFKDEYYLGGQINLYPIELAQ
                     ILSEEVFVMHSRPFAPPIETNAIKKVYNYDPNLRLKYTDSLIRESDYWIDASDFSSEL
                     AQEEVSSWKVRVKMLSYEDYVSTSLKHYSQGKERFLEALKNKGSLTHVRNLQK"
     gene            complement(18364..20085)
                     /locus_tag="PLF66_10120"
     CDS             complement(18364..20085)
                     /locus_tag="PLF66_10120"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HMZ64440.1"
                     /translation="MLQRKLLLLFAAIYPCFWALFKYTKAPIDDPKQRIIVSILCLIA
                     FKIYESPNPKIRNRQSLVSYLAYYTVTLHSLYLSYVNQFSVEFQIQNFTIIIFASLGF
                     IRKYHLFYYLFFTFFCFVPITVAGFNKLDIYYILCNLLIVCAGAFTVQMLQINFLKQL
                     LVNDRILLKTMDKIRDGVIITDISSHVLFVNENAQKLLNFNSEFLIGSKVNLPIPSEN
                     RLTGNTTVIHTVEDRLVEIRMLKVERLGKPAFFIIIKDITKETREKDEIERKRILFQN
                     VLTNTKEGIIGLDKEGKITFVNPYTLELTGYTEEELLNQNFHETFHHSWMDGIPHEEE
                     HSPIKETLIEGTTNRITNEIFWKKIDQFFYVEYTCSPIRKENEIIGATIVFKDITEKR
                     KQDEIDKKYNAELEFLSESAMNYLQLNSKKEIYHFIAESIFKFTKPRAVIVNSFYLEN
                     SQFQVEVTSGFKQFAAPIQKLINCDLSDLRFEFDKERFLHSQTYNKLIFLPDGLFTVN
                     FGDLNRKECMMIESMLNANRIYHFTIADSNRVLGSVIIFNSEEKFQFETTLEIFLHQA
                     CIALARV"
     gene            complement(20089..23184)
                     /locus_tag="PLF66_10125"
     CDS             complement(20089..23184)
                     /locus_tag="PLF66_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012390025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HMZ64441.1"
                     /translation="MKFLTLIVEWSLENRVIVLAATIIFFFFGLDSARKLKMDAIPDV
                     TTIQVQVITSAPALSPLEIEQYVTFPVERSMSGIPHVEEVRSISRYGLSVVTIVFKDS
                     MNIFLARQLVSERLSDAVNNIPKGYGTPMMGPISTGLGEIYQFTLSSRNHSLMELTTY
                     LNWYINPILKTIPGVVEVNTFGGNVKQYQIVLNMERMQSLEIVMSDVIEALQKNNASM
                     GGGYIEHNKEHFVIVASGLVGSLDDLGKIFIGKTKEGTPISLSMIADLSYGAKLRLGA
                     TTKDGGGEVVGAITLMLMKENSLHVSEAVHTKLEDLKKNLPEGIVVETYYDRSVMVNT
                     TIKTVLKNLAEGALFVILVLFLLLGSIRAGLVIAVTIPLGMLFAIVIMRIRDVPGNLM
                     SMGAIDFGLIVDGAVIIVENAVRRLSMAIKEKGSLLTDAEKVNTIKEATIEVRKATIF
                     GETIIAIVYLPVLSLSGVEGKMFIPMAETVLYALFGAFILSLTVVPVLATYILKVEEP
                     EEHETWLFHKFKTYYIPFLDKIMNAKNKVLTVALISIVMSFILFANLGAEFMPELDEG
                     SMLLEVARLPSVSLSESMETGRRIEKALKEKFPELVHAVSRTGSPDIATDPMGIDRTD
                     IYLQLKPKEEWRFEREKLMQALVENVEESVPEVAVSVSQPIKMRTNELIAGIRSDIGV
                     KIFGEDIKTLREIGEKTAAELRKIQGVVDLKIEQLGGLNYLRIKPKRDSLVRYGVSIT
                     DVNQVTEILSSGYIVGSVFEGNKRFDIAIKTNKKVLFNLEEIRSLPVRSSAGNAIPLG
                     DLADVYIESGPVQVSHQNQYRRMIVEFNVRGRDMMSVVQEANDILHKNIPLPVGYRFE
                     FGGKYENYISAKNTLLIVVPVTLALILFLLWLAFGEVTPALMIFLNVPFAITGGVLFL
                     FLRNIPFSISAGVGFIALFGVAVLNGLVLISFTKEQEHLGLSHLDAVVSAAHIRLRPV
                     LMTAIVAATGFIPMAVSTSMGAEVQRPLATVVIGGLITASALTLLVLPVVYAKYYELK
                     K"
     gene            complement(23187..24365)
                     /locus_tag="PLF66_10130"
     CDS             complement(23187..24365)
                     /locus_tag="PLF66_10130"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HMZ64442.1"
                     /translation="MKYNKIYTLLAILAISFTSYRAYKYFSKKKNTEATETNSIKEDT
                     SIKIDSEVSKDVNLHSSQVGTVEFQETISLIGEVMADPDRVTKVSARISGRVTEVRFI
                     EGVRVRKGQILAVIESPEAARSRSKYISTFSRVNAVQKNAKRIRELVNLKLAAEQEAI
                     NAESELKIQEAELKADKGNLHALGIPIPDINSMEGSGNENSGRIEILSPIDGVVLTRE
                     VVKGSQVDAISNLATIGNLDSVWFMVKLFEKDLGKVSEGDTARVKLNPYPEEEFEGRL
                     TYIGNQIDPGSRTVSGRIVITNKNHMAKIGLFGKAELYISDYDVIAISVDAIANIDGK
                     DFVFIEEKPGEYKPREVKLGRRNKSIVEIVSGVQTGEYVVDRGIFTLKSTFLKSTFGE
                     "
     gene            complement(24373..25686)
                     /locus_tag="PLF66_10135"
     CDS             complement(24373..25686)
                     /locus_tag="PLF66_10135"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HMZ64443.1"
                     /translation="MKSIYIIFFLVLSFYPIYRIHSNSDSHESNDHCAGNAKLLTIVE
                     CVLTHSPEFRKTRIELTAIKGKKIAAAYLFPSNPSIQVMNAYRKQAQSEVSFLNPVPQ
                     TAINGEVQASQEFYLAGQRGKRIEVADTEYAAQIKKVASMERDTTAQALAAALFYKNS
                     LEEFKITEFLYQNSQEMAKAVQARADKGLAAAIDADIANAEVSKTARLLNSAIRKRDG
                     AKGNLMVMMGVNFDLALSIIDTIPDIQLNTKNIQELITLALKQRTEVDSGVLDVQTAQ
                     RRIELLKRESIPNLTVSAYLQRDGFNENVVGARASMPLRVWRDNSGEVVESEARKEQA
                     ITNLEINQHTIKYEVIRAITNFNSLKEENKNYPPELMKKVDEDLISIKKALSTGQMNI
                     RDALIAQNSLVSLKLSYIQTQSDYDLSKIELLRSIGFPLLQYIVK"
     gene            complement(26360..27364)
                     /locus_tag="PLF66_10140"
     CDS             complement(26360..27364)
                     /locus_tag="PLF66_10140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017861842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HMZ64444.1"
                     /translation="MTHLYKVLFALSFSVVSFGFLYPMYGDILQPAIYTVVILIATLG
                     VLYFEDKVFPIINAEAAFCVGIGLLVGFGIGRLLLTLIAESKNPGMGFLVYLGFGYAG
                     IKVGYAFSKKPGLSIFGGGGAPIRPFILGGEKPEEIRDKILDTSVVIDGRILDIADTH
                     FIDGPLILPNFVLREIQLISDSSDPIKRARGRRGLDMLNKLQRKGTIEVKITYTDYTD
                     TREVDAKLIKLARDTGAKLVTNDFNLNKVAELQGVKVLNLNNLANALKPVVLPGEEIA
                     IQVIKEGKDENQGIGYLEDGTMVVIENGGHLVGKDVRVNVTSIIQTAAGKMIFTKAIK
                     "
     gene            complement(27404..27961)
                     /locus_tag="PLF66_10145"
     CDS             complement(27404..27961)
                     /locus_tag="PLF66_10145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010573325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HMZ64445.1"
                     /translation="MRSQNISDIEFKVGDYVVYPMHGVGAITEVSKKTILGKKRDCYS
                     LEIQSSKMKVMIPVDKAKQVGIRGIIPKREIKKVLNLLMKDEVDTEEDWKIRYQNNMN
                     KIKSGSIYEVADVCRNLFRRANGKELSIMERKLYENAYNLVKTEIALSKGVPQEEAGN
                     IVSDILASSVQPQMIQQAEPTEDVE"
     gene            28407..29000
                     /locus_tag="PLF66_10150"
     CDS             28407..29000
                     /locus_tag="PLF66_10150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64446.1"
                     /translation="MQSKKMIPSDFAIKRQTYVQKDLTAGSAFERYIGSILEKMSYRV
                     EYPNSEVNAESEAYIQNYASIQIQLAKALDIQAKVTEIKNLYFECSWRSIGYFLKKKT
                     HTGIPLFKYQNYSENRAPETFIIIGHGGRPENPEKVFITTLQKFSDFGETIVYDLFKG
                     NKARHSPHQRALALKHFKILERSDEMEKYFRDSIQNL"
     gene            complement(29142..29819)
                     /locus_tag="PLF66_10155"
     CDS             complement(29142..29819)
                     /locus_tag="PLF66_10155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HMZ64447.1"
                     /translation="MQNVDYSRKLRRDDKRSHSTEPRIPLKTSKSSNNGSILNLLNSS
                     HQPLSFLYLFIGAILFFTSGLVIGMKIDQKESYFMGNESNTFRNVNSSESNSSMEAVE
                     SSNTKEIKAENQDNDGFTGEVSTNKVTKTALPTIHKDLKFPPKLNQVNYIIQIGTFSR
                     EEANKWGASLIKDQQDFQGRLFRTSTGKLYLGYYYNIKDAKLVLKQVKKFRGGVFEEA
                     SIKNIQF"
     gene            30161..31720
                     /locus_tag="PLF66_10160"
     CDS             30161..31720
                     /locus_tag="PLF66_10160"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF016362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HMZ64448.1"
                     /translation="MNVYLFVAVVLALGILGVIYFIFFGTKANIVEKALALAAMGNFI
                     DAKATLRDQLDLEPNDHKLLYTFSKIYAMENDLLNEVSYLEKVKSIGKYEKEFPPLVV
                     NNRIASIYYQQDMFDEAFFYYLDSLNYDPVNLEALIRLSFMAVGQKDFDIADKFMRQI
                     PDDEVKIQSYFIAKGVVTAMLNRDNDFEYFEKAYKIDSNSPVAAFLYALSLSKIRKFK
                     DALDIMLPMQDMGGDDLVRFTIAQFIMTLNSSMSDYVSAQASAKTCIEIAKRNNWHLE
                     TAECNSYYAMLCIILNNLEEAHEYLIEAEAERVDDYDIISLAQYRSDLEDGSATPGKT
                     SARGYNLKNALKDLPEKLFPKERYFEISGLKAAEVINIRGIINQDGQKIISKLSQLSP
                     DKISKFNSQKGNVFKNTCIKILAEYGYKVKRELPALEAEGANFVTIKRDDESDRAVFR
                     IRKWKNMTISDVFLTELLNSMHEQQAEKGYVVGSAELTPGAKKVIRANEGKIMVVNGK
                     DLESLLERVIK"
     gene            31885..32418
                     /locus_tag="PLF66_10165"
     CDS             31885..32418
                     /locus_tag="PLF66_10165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64449.1"
                     /translation="MLKKIILIFYIIFSLSLHAEPDFVSIHIRNVYSNGTKLSPMDSK
                     RILSVLRGLRDAVLEKDADKILRYISKEKGAYLDVKGLWEYDRILEEAKKEDSYFEVY
                     FFDRQKLVKQKESEDVFTVRELLLKSDGIEIDLFFDSENACEVKLIFKNNTKLEKDMN
                     NPYLVKVNGRWFIYRLF"
     gene            32536..32850
                     /locus_tag="PLF66_10170"
     CDS             32536..32850
                     /locus_tag="PLF66_10170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015679182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HMZ64450.1"
                     /translation="MAVKKAKSITQVSSSKQDWTFLSNHAHVLICLYRDPTARLRDIA
                     FNVGITERAVISIIEDLESVKIVARSKDGRRNKYKIQETISLRHPLEAHKNVGDLLKL
                     LK"
     gene            33482..34159
                     /locus_tag="PLF66_10175"
     CDS             33482..34159
                     /locus_tag="PLF66_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015679293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HMZ64451.1"
                     /translation="MKKSIFFMMIFSIVNSLLAQATVSPDEALKRLLEGNKRFIAGKS
                     IRPNQTPEKVKELAAGQNPFAVIVGCSDSRVPNEIIFDQGLGDIFIVRTAGQVSTYAS
                     WGSMEFAVTSLGAKLILVLGHTKCGAVNAACAVPDVPGHIVTLINAIKPAAEIAKEME
                     GDLLDNAVKVNVAKQVEQLRALEPVLAKKVKSGEIKIVGAVYQLETGKVELVPDNFLK
                     KISKEKK"
     gene            34159..35133
                     /locus_tag="PLF66_10180"
     CDS             34159..35133
                     /locus_tag="PLF66_10180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002975637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent bicarbonate transport family
                     permease"
                     /protein_id="HMZ64452.1"
                     /translation="MEIFSSLFASLLTPMVLAFVLGIFATLIKSDLKFPEGLYIGLTI
                     YLLFAIGLKGGMKLSATSLADFYKPALAAIVMCCLIPVWSYFILNKLGKFNISNAAAI
                     AAHYGSVSAVTFSESLAFMEVMNVSYEGFMPTMLAIMEVPAILIAIFIARTKEGNNDS
                     SWGKLFHELFAGKGTVLLIGGLIIGLLTGKKGYEQVAPLFDTPFRGILALFLLEVGLV
                     TGKRLGDLVKAGPFLIGFGLIMPVIHATLGILFGKYVGLSMGGATIFGVLCASASYIA
                     APAAIRIALPEASPTYYLTASLAITFPFNIVFGLPLYLTIAKNVFGVN"
     gene            35133..35435
                     /locus_tag="PLF66_10185"
     CDS             35133..35435
                     /locus_tag="PLF66_10185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64453.1"
                     /translation="MNLHPIQLITIISLDVLEDKIINDLKQLGVKGYTVSEAHGEGLS
                     MRRDDNWEGRNIRIEILANSELTEKIFGLLQKEYFPKYKMIAFAQEAKVLRKEKFN"
     gene            complement(35465..36220)
                     /locus_tag="PLF66_10190"
     CDS             complement(35465..36220)
                     /locus_tag="PLF66_10190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64454.1"
                     /translation="MPTNKTSRSIVSSTPLNDRPMIYAKFVLFAILLFQQSLISKERT
                     EYSEIKSFLERKDFISSQRKINSLLSDNPSDPELLYLQADTWFQQAESHYKKENYKTA
                     NDLYKQVLGIWPNHPILNERISFLSKKNLTDNNQKKEESVIQNPLANHSNINETYIVV
                     NEDISLFKIVSYNQEQDLWKSELSFPMKNPGYSIITLKASEKEKLILLKPIAFGILIL
                     SVILTTLSVNLILLAMKTKKSSKGIWSKKKGNT"
     gene            complement(36263..37819)
                     /locus_tag="PLF66_10195"
     CDS             complement(36263..37819)
                     /locus_tag="PLF66_10195"
                     /inference="COORDINATES: protein motif:HMM:NF025330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HMZ64455.1"
                     /translation="MTLEKIVSSSSGIQVCVSRELQVKQGFSFLAVLDDARKSCAART
                     DISFLQWLSICIAFLFYLHCSNSPVNKGNETETLKLEITYVNEITTTSAIISWTCSQE
                     AEGRLLYGKNIANNTLYTPFKSKLHRFNLSNLTQDTTYKYLAFCGNTLQTFLPLNFLL
                     TFKTLKQETPPPSPPSMDEIYNRGIWILGGLTTSNSFISQVDVYDPVDDLWYPQITNI
                     PTPRSHAGIVSYGGKIYVIGGLFLHSSNQIAATNLVEEFNPSTKTWRTMSNMPTSLQG
                     FVIGVGGNSIYTIAGTTTINMASGTLLNTVYKFNPDIGATGTWSTLVSSSAISQKVDM
                     GGCSINGVLYFNSGRLFSDGSVQSTNDGYIFSSNSTTGITETALNVGRHGAGSACYIP
                     KSTDSFPSDSPAVLIVGGSTLLNTAQPVTSYIVSAAYDYYLPNSSGVNPNTTTSGPAL
                     PVGLYYPAVEFSYSKRRAYTFGGLLSSGVANDSFYYLDMANPVGGQWTIATTKMPIAR
                     YAHKAVILNR"
     gene            complement(37816..38673)
                     /locus_tag="PLF66_10200"
     CDS             complement(37816..38673)
                     /locus_tag="PLF66_10200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64456.1"
                     /translation="MKLSARIKIISFLLLSSISLSAAEIVLKNGDAFIADILENDANK
                     VRIRWKEKTFDIPKSEIQNIDTEKKGEHISYHYDTIILKDGTQIKGVIVRQNTNDLIV
                     KTELGLLTIEKSKLENAKSLIPDKYNVDLPPEKNSNIGLTGSYSAFAQSSNNVNQASG
                     GGLFIEPSFVHFNSRWRLGYKLEYLESINSRFRFYNNFLYLQYNWKKSNWIDFYANLG
                     IGGSIVNYKYTDKTVTGSDPAGYLEVGWGGLSFGKVIIRLGIIGIYLYENGGGPGFGG
                     GTISIGAQL"
     gene            complement(38811..39494)
                     /locus_tag="PLF66_10205"
     CDS             complement(38811..39494)
                     /locus_tag="PLF66_10205"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HMZ64457.1"
                     /translation="MNQKRKRLSRPKTLVIFGIIFLILPFLNYYLLSRQLGVSPQNFR
                     IILFHSDLFALFLMLLPIPVGIGIINVKRWGWQLFLVYASLLILYNLQVFVFNFKLYN
                     FAALFNSILGFASIAYFVRKDISAPYFKMYPRGWRLQKRKPIEMKISVNGKELKTTDV
                     SSTGVYALWSDCPYELNQAVNVKFALENNVYDLQAGVVRVDENGMGLAFRGLPKLVSK
                     ELSSFVKEL"
     gene            complement(39509..39871)
                     /locus_tag="PLF66_10210"
     CDS             complement(39509..39871)
                     /locus_tag="PLF66_10210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64458.1"
                     /translation="MNKLILMSLFTIATISISAEQEVFKSCVSGEREPSAKEANSFAC
                     IESDNPNLSKWCAKMAEGNSLTKKYKSSGKSCLDLGFTSKPVKMPLGNSYTCNFALPK
                     FNECKDSTKQILLQTIQF"
     gene            complement(39888..40286)
                     /locus_tag="PLF66_10215"
     CDS             complement(39888..40286)
                     /locus_tag="PLF66_10215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64459.1"
                     /translation="MKYQYKIISNLSINFSIEPFYTYGARNIKRDIIQPYGSPIPSAN
                     DFRATYNDPHAKIYFYGAEFDINLSFNIYENLNLLVGYGFIYTKIRYENDRRINYTKY
                     YDIINYPSYGSYIHSTDHINNWYIGLNAKF"
     gene            complement(40361..40933)
                     /locus_tag="PLF66_10220"
     CDS             complement(40361..40933)
                     /locus_tag="PLF66_10220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64460.1"
                     /translation="MKKDIQKKTDFKKHLTRGFFLILVLIVINTNIFSQEEKNIDNNK
                     ENKLSEKIHQFGFTIKRANYAYYPVEYENDSIGKTLLGLWDRPGLRSQSELFSAANPK
                     KLSPMRNNEQVINPFSLLYYNKNLNLNFEFSRYSLVLDSPYYVADLFGSSITPHDIGN
                     IRRSENKLNVYSNIKLNESNSFFMGLVLEI"
     gene            complement(40952..41563)
                     /locus_tag="PLF66_10225"
     CDS             complement(40952..41563)
                     /locus_tag="PLF66_10225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64461.1"
                     /translation="MKSVLILLFFIQTLSASDSITFSGSMNSELNPNFRFGRTIDYND
                     TLPLAASSKWSLGAAKTLTISVLDINDNPVAVPLMYHVIKNGTSKTPSFTTCSGGCKI
                     SWQNAYSIQFAVNGGVPPYKWYTATNAPPEANLPSGSLPPGASMNADGLLTGPGTNNF
                     MPNAVWGFAVLVVDSVGQMDVKVIVLDSTDQAAQYAACLLLGM"
     gene            complement(41579..42523)
                     /locus_tag="PLF66_10230"
     CDS             complement(41579..42523)
                     /locus_tag="PLF66_10230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64462.1"
                     /translation="MKKLIILTILFCSQVIFSEEQNKELNTQKKYSLTIKSAHFSYLP
                     TEYLDYRERNTLGSNSASFLHNNRTKTNPLSFSYYSKDLNLFFEYSYYKISLNNLFYT
                     KTEFLESSPGQLRPFFLGYTLPTITRRENKLNAYKKYSVNDRNHFFFGLGIRNIFKEV
                     SSNLYSNERYIRTDTYGMEIFLKYRLDFLSSFAFNLTFEPFYTAGKYITKDFPYNNSI
                     NKKVYLFPIVNDSFVYFWGYDADINLSYRIDDNLSFFFGYNYIRTATRVQNRYQDNVS
                     NSYYIFDSNGLRYFNQRDSYEKTDDNIQNYYVGIKAEF"
     gene            complement(42553..43731)
                     /locus_tag="PLF66_10235"
     CDS             complement(42553..43731)
                     /locus_tag="PLF66_10235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64463.1"
                     /translation="MKLKKFLFILFLTIFFVTFSNAGCSGCSKEKKDKLIEFVVGILK
                     QWADDCSDGIQHNPLCPKPPASSVPCLCEAKPCTTVLIGAKFTDLNCTPGKIRGYRQY
                     DSAGNEIPGGTTYDWITLDKFDGEANKCGVAYGEPKQVFQSACTNGRKSIQENQYLQG
                     YDLLSGSPPVGVKSTDPQNAQVIFESQQMLTIYFQNTVKSDNLQFSGDLSLLIDPNYK
                     FTRTTDFNDTLQIKSSSKWSLGSFRSLNLSLFDIDDKPVSISLFYHIIKDGTSKLPSF
                     YTCVSPECKLSWQSAYAIQLEANGGVPPYKWYTINDIPTALTVSLPPGFGPGLPPGAS
                     VSREGLLSGPATNNFYATYYFGILVVDSIGQTYPAVVVIDTTDLVAQYAACLLTGFCP
                     "
     gene            complement(43748..45028)
                     /locus_tag="PLF66_10240"
     CDS             complement(43748..45028)
                     /locus_tag="PLF66_10240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64464.1"
                     /translation="MSQFCIGSKSSDSSSYLSLAGLLTSSPQSQTSSASGSPPYGSLP
                     TGSTPTIVGVPVSVMEQPTIMNGYQTNLNLLDLLGIFYHRNPNFPEVRQITDVSVVKL
                     NKDLEKSEQLDWDFNQFILDGKMGLILFTRGLQQLDYTQNPELKGATQISVQVNGKIA
                     ILNANGNINLCYSFDGNSVIRNPSCNTTALSSVKIAPHLKDSFISFDANGNFSSIHSV
                     TGDIKALDFRKPLPNPISVSTGEDVILIIYNGGTSIIAFLDTGADSNSRFEEKANTNL
                     IQIAIDGDLNNLTAPLQVTSIVRSFEGRYYAYTPTLNALISFDPTLKVHSGIWEFPSV
                     PIPFYRLKNVVGLQMHPNSRLIHAYNLYAIHTFSETSIFDREDLGRYIPPSFDAVLNF
                     MSESINPDNYDPSKDLFKYPGVEQFLNVQKQNYL"
BASE COUNT        14449 a         7785 c         7521 g        15453 t
ORIGIN      
        1 aactgttctg aaacttcttt gatttgatcg tatccttgtt ttattttatg actcaactgc
       61 cttatctttt ttgagatcaa ttcagaattt tcatttgtat gctcaccaag cttggaaatt
      121 tctttggcaa ctatttgaaa tcctttaccc aattcccccg cgcgcgcgga ctcaatcgat
      181 gcatttaatg aaagcaatcc aacttgatcg gatatttcgg aaatcaattg cgcataatca
      241 ataatagcct tagattcttc ttggatttcg gaaatggaat tttcagtatt caatcccaaa
      301 ttagccccta cttctgcata ttgatttgcc ctgcgagaaa gctctgctga attttctgct
      361 tgcgttagac taccttgcat ttctttacct aaatcattta gtgccaagga gcaattttcg
      421 gatagccgct tctggacttc tgagttttgt tctgtttggg tgattgctgc ttgaaattct
      481 tccatactag cagaagttcc ctgacatagt tagattggtt ttctgcttcg gtagatacat
      541 gagtagcatt tagttctaag ttctgggaga gaagatttaa tttattagaa gtagctttta
      601 gatgatgtat cacttcaaaa atattctcac tcagagagtt gaaattaaga gctaaaacaa
      661 aacccaactc ctcactatca acgagaggca tgcgataggc gagatttcca ttttttaatt
      721 ctgccaatgc ttcctttaca ttttctgtat ttttacttaa aattttagaa agaagatttg
      781 cacagataaa actcaaaacc aaagactgaa atccaatgag cccaactaga taattccgaa
      841 aaggatcgtc taaatcagat aaacttccaa agtaatagag cgatatcaga attccgacag
      901 gaagaaagag aattgaaaaa cttgcgatgc tcactctatg taaaatagtt atcgcacgaa
      961 tatttttcgt atctaccggt atattgcgaa tagtcggtag tgttaagata tcagaaataa
     1021 ataaatccgc ttgcgtagaa tgaatcatat acataactgg tgctaaaata actatcaaca
     1081 atacaaacgt aattacacca tacaaactca caggaacaaa ggaataatac acagtggctg
     1141 tagtaatcgc tcccaaaatc cattgtaatt gaactacaat cacacattag gttggaaatt
     1201 tgagaagatt ctctttatgg aagcttagct ctgaatcctt tgtgcctact agcaaaggat
     1261 caaacaaact atgaaaaatt tttgctctcc aataagggaa aataattagt gtaatcgaaa
     1321 cagcagctag aaaacttagt gcgatgtaat aaaatttttc ataatacatc ttttgagata
     1381 aaactaaaaa gaaaacagca agtgggacgc cgcccacata atttgtaagc tccatacgaa
     1441 tacttaggct gatgatttgt tttttaattt tttcttccat tgaacacctt agaatagaat
     1501 ggatagccac atttttatgt aaacaaaatt aatattatag tattattgga atttttaatt
     1561 atatgggaac taaccctcta tagtatctaa cgattagaat agaaattagt tcaaaatatc
     1621 tctttctaaa agactcgcaa tccttttttg ctttaagcca ttcacttgta agatagaatg
     1681 ggagaagttg gatgcaaatt aggcgacagc cgctagaaag ataaatactg gacacatttt
     1741 tgtaatagaa aaatgctaaa ctactcagag catctcctat aaaaaaaacc ataatctttt
     1801 ttttgttaaa atggattcgc atgtaaaata gaatggaaaa tgtttttgga aaaagttgta
     1861 tggaaattag tagaagaaaa gtttgaggta ggaaatggat aaaataataa tttttgatat
     1921 ggatggagtg attgtcgata gtgaaaaaat ttatacagat atgaatcaaa aatggtttac
     1981 tgaactagga tttaaattac caattgaagt tcatcaaaag tatataggaa tttctgcaaa
     2041 aatattttgg acttttttga aaaatgaatt caagctgaaa gagccaattg aatattacat
     2101 tcaaaaagaa aaggaattaa aattcaatac tctatcaaat ttacatttaa ctccaacaaa
     2161 taatttaatg aactttatct catacattaa atcaaaaaaa tataaatgtg cgttagcttc
     2221 ctcatcctta aaaatgaata ttaatcttat tttaacaaaa ctgaatttat taaatcattt
     2281 tgattttgtg ataagtggag aggaagttac ttctggtaaa ccgaatccag aaatattttt
     2341 acgagtaaaa gatttttttc aggcgaatgt aaatcaatgt attgttattg aagatagtat
     2401 gaatggtgtt accgcggcaa aagcggctga tatgttttgc ataggttatt ttaatcctaa
     2461 ttcaggaaat caggatttat ccaaagctga tttaattatt gatgatttca atcatccaat
     2521 gctttatact ttataaagtg aaccaattgg gaaatgatat tgaatcaaat cggcgctcaa
     2581 aaaattcacc tatctacttc aatgcgcatt tttttaaaag tctcgcaatc cttttttgct
     2641 ttaatccttt cacttgtaag aggagacgat tatggaagaa gttcgatgca aattgcttta
     2701 aagaaaacta ctaagcagtt ttttttaaag tttgaatgtt ttctaatttg tctcttaact
     2761 tttctttttg ctgccgaatc tctaaatcat tttgcaaatt aatccaaaat ttatcagaca
     2821 taccgaaata cttagctaat cgtagggaca tatcaatagt aatcctcctt ttattatgaa
     2881 gaatttctaa aatggtagaa gtagaaacat gcaaatcctt tgctaaccta tacggagtaa
     2941 tcttcagagg ctccaaaaac tctactctta atatgtctga taccctcggt gttggaattc
     3001 gtttattttt cattagtaaa acctcttagt gataatcagc aatggaaacg ttatagcagt
     3061 gcccttcttg aaattgaaaa gtaattctcc attgtttatt aatcctaata gaacaaaaat
     3121 ttttcctatt gccttttaaa tgctcaaagt tatttgaagg tgggaccatt aaatcattct
     3181 ctgtttgcgc attttccaaa agcaatagct taactaaagc tctgctttga atttcaggtg
     3241 gaaatttttt agagaattct tggctaaata ttttttctgt ttccttatct ccaaatgact
     3301 ttatcacaaa gatatattcg tttatcagat atatctgtca agcagttata tttaatttat
     3361 agaaaaataa attttgctac tcaatttact aaaggagatg cgagcgtcaa ccggagacaa
     3421 atgattttat taattcttta gtttcagaat ttaattacaa aaaatttccc gcatctgatt
     3481 caaaccatct ctttctaaaa ccttccactc agcgtcccat accagatacg tccagggaga
     3541 ggataaccaa caatatcctc tgctcgtttg tcggtgatgt tttttacttc gaaggtgagt
     3601 agcaactccc gattgtcctc ttctgttgag cgcttgaaat aataggtaaa gaaagtattg
     3661 taaatccatc ttgcactttg atagttttga tattcattcg ctctgtcttt gaaaactgct
     3721 ccaataaaat ttgtttcaaa tccgatttca gctctattaa atctacgact tatacttcca
     3781 tgccattcgt tgactggtct aagaggaaga tattttccct ttaaataact tgtgtcagat
     3841 gtattaattg ctttttgata tgtgtagtta gattccaact tccatttgtc gaataacgtt
     3901 acattttgag taaattctat tcctttaata cttgcagagt caatgttttc tggtctaagt
     3961 gtgaattgag aatttggaat aaatagaatc atgtctgtaa ttgctttttg gaaataaacg
     4021 attgaacctg tatattttaa attttttctt tgggtatcaa atacgaaccc cgcatccgta
     4081 ttaatacttc tctctggttt taaagataga tttcctatta cagatcctct ctcgccgaat
     4141 aattctaaaa aatcaggaat tcttctttca cgtgcaatat tagatttgaa gctaaaagta
     4201 ttttctttgc ttttgtggat aataccaagt aatcctaaac gatagttggt gtattgtgtt
     4261 gtcttcttgt tgggctctgt attcgtattt aaaattagag aatcttggga gttgaatcta
     4321 tcaatataaa gttcttgctg tattgccggt gtgattgtaa gccttttatc taagagacgg
     4381 atttcatctt gaatttgcca agtagtatga tgacgaaact ttctggtaga tttatctagg
     4441 acttgatcta acttatttcg tctatctctt aaaaaagttt ctctttcgaa agcgaataaa
     4501 aaacgaagaa tttgatttga attcaataaa taaagagtgg gtaataagtg taatccgtat
     4561 tgttgaatct ccgcttttga gtttggtgtg ccagatgaaa attcagagag tggatcaaat
     4621 aaatgatccc ttgcccccgt gtaaaatagt cttgtatcta aattcaaatt ggaaaataaa
     4681 aatgatttag acatagatcc gagtgaagtg gtatttctta aatattctct ttctactttc
     4741 tttgtttgat tgcttcccgg tcctggaatt ccgttttttc tataattaaa atcatttaag
     4801 atggttacag tcgtctgttt tatatcgaaa gaagcatttc cagtgaagtt gtagcgattg
     4861 aattgagcat ttttacgttt atcatcaaaa tcatcaaatg aatttagaaa agttccatta
     4921 ttgtttctaa atgtaaagtt ttgatcagat ttttcttgtt gggcgaaaag agaatatcgg
     4981 acgttatgta tcgttgcatt gtgactcgca cttagtttaa atgtattcaa gcttccacct
     5041 gatacaatga atcgagaaga aggtttcttt gctgatttac tagttactaa gttaacagtc
     5101 cccccaatag ccgaaccgct gaaaccagga gatgcgccgg atttatagat ttcaattttc
     5161 tctaggttgt cgaatgataa atccgctaaa ttaatctccc caccttgcga attgtttaag
     5221 ggaacaccat caatataaat tcttacttga tttgcatttg atcctctaat ggataaagta
     5281 gagtaagatc cgagaccacc aaagcgcctg attcgaacac cggcttctcg ctcaagtata
     5341 tcggttaacg atgtatattt gtttttaaat ttttcagcat caatgatttc tgtgaatcca
     5401 gtaggatcag cttttacaga tttagtttta ctagaatcat tctgtgttga tgttcctact
     5461 acttcaactg tctcggatcg tttaatatct tgtgcaatca gttgccctat cgataaaaat
     5521 ccaattagaa gatagcgcct caaattaaaa tacattaaat ccaatttcta aatagaatct
     5581 ctagaaacaa ggaatatgtt tttgcggaaa tattaaattt ctgatagaat tgatttttag
     5641 ttttactttg atttataaat tctaaaataa tttaccaaag aagctatttc ttttccgaat
     5701 aagtaaatac aaatcatcca attctttttg attttctttc atgccttttc taaagggagt
     5761 gatatgaaat ccaaacctac caatggattc agtaaattga atttctaaag ggtcatcatc
     5821 tatgagcaaa tcaccattta ttttacgaat atcctttttt attcccttat cgtccggatc
     5881 gtagtcttcc ctaaaaatac aatccctgaa atattctttt aagtgtagtt ccgacaagat
     5941 ggttttggct ctgtcttttt tagaatttgt ccaaagtttt aactcatgtc cttctgactt
     6001 gagtttcttg agtaagtgaa tgattccagg tcggacactg cttccaagct catcaaccaa
     6061 tgtattatcc atatcaaata caagaatcat attttaacat ttgactttgg tcataagaaa
     6121 taaaagcata aaacaaaaat gaaagtaaaa acttgtccag cttgtaaaac tgaatttatt
     6181 tgtaatatgg aatcgataga agaatgccaa tgtggcaaag tagtgttaac tgctcttcaa
     6241 agagaaaaaa tagcgaatct ttatcaagac tgcatttgta aaaattgttt ggttcgtttt
     6301 aatttagaag agataagccc gaattcaaaa acttcgggct aaaatttatt gttcaactat
     6361 gagtgtgact tcgatgttat ttcgtgtcgc attcgagtaa ggacatactt gatgtgcagc
     6421 agagaccaat tcttctgcaa tcgacttttc catcccagga attctaactg ttaaggtgac
     6481 tgctagacca aatccgccac cgctaatcat acctatatct actgacgctg ttacagaaga
     6541 gttctctatt tctagtttct tttttgttgc aacaaccttc atggcgctgt gaaaacaagc
     6601 agaatagcct gccgcaaaaa gaatttctgg attgactcca tctccccctt ctcccctcaa
     6661 ttcctttggc attgatagag aaaattcata tttcccatca ggagatttta catttcctga
     6721 tcttccacca cttgctgtca ttactgctgt atataatttt ttcattcgat atccttttta
     6781 gaaagcgtta tttaccatac ttatcatctt tcatgattcc agatgtagtc caacctttag
     6841 gagtatgaca ggaaagacat gttttaagca tgggagattc tttaacttta gactcaaact
     6901 caggcatagc ttcatttttt acaattaaat ttttatagga actcattgcg atatctgcat
     6961 tgtctttttg aaagctacta ccatttcctt tttcaactaa ttttggtttt gctccggtgg
     7021 ttgctgtcgt aacttcaatt ttgccttcac atacacagtg catagacgag tttgtttttg
     7081 catcataaat tgctccgaat gcagttcctc taactccagc cgttgaagtt ggagttgtgg
     7141 caatgaactt ttttccacct aagtctttta ctttaaacca agcacttcca ttttgcactg
     7201 caatacttcc gtcattttct tttgtataaa ggctgtttac aacgatactc gaatttgctg
     7261 caattttaaa ttcagagcct ttatatgtaa gtgtagctat agatccattc ccagtcataa
     7321 tggaatcacc actacttact ttgtcttctt tcttgagggg cttccacttg ttatccgctt
     7381 gggttttgat gtttactttc ccaactaaaa agctaatcac tgcagtttca ctttcttgtg
     7441 cagatagaga gattacactt gctaatacca acacgatact aattaagttc atagattcat
     7501 cctttctatt cttttttgtt ttgtttgtct aatctataaa acaatcaatt ctttggaaaa
     7561 tattttttta ttttccatat ttttcgtctc gtgtaaatcc ttctgctttc catccgtttg
     7621 gagtatgaca ggacaaacaa ttttttaata ttggatatgc ttttactttt ttctcaaaac
     7681 caggaaagga ttctccttta gccatctcac ctttataaga tattggaatt aagtttgagt
     7741 cgcctgactt tacgctaaca ccatttcctt ttagaacgat acctggcttt ccttgcttta
     7801 cagggtttac ttctacttta ccttcgcaca cacaggttaa atccatgtta gccgttgaat
     7861 cataaacaga tgcaaaggca gttcctctta cacctgcact cgaagtgggt gttacagaat
     7921 taaattttct atctcctaga ttgattaatt tgaaccacgc aagaccttgt tttacttcta
     7981 ctataccatc ttcgttttta gaaaaaagtt ggtttacgat aatagttgaa ttttgcgtta
     8041 atttaatttc agattcttta tatagaatcg ttgccataga tccatttcca gtcatgatag
     8101 aatcaccctt ctgaactgta tcatttactt ttaatttttt ccattcgcta gattctttag
     8161 actttattga aactttaccc aaaagacttg ctaccttagc tattgaatca ttttcctgtg
     8221 ctaaaaccga actgaaagac aaaagaatga tacttactgt gacttggact aattgattta
     8281 attttaaatt cataccctat ccttgtatat attcttatac aataacaaac tatttattat
     8341 ctattatcat gctttctctg attattgaaa tttagttttc agaataaata gatttttggg
     8401 atcaggagga aataagtagg aaattctatt aatgcttttt aattataact caccttactc
     8461 aaaatttata aatctaggaa taaacaatga tttttataat attagagaat gctaatgatc
     8521 taacaaaatc tatagcctgc gatagctcgg ttgctataga gcttataaag tgatgggtgg
     8581 aaaaaaactc tctgctagga gcaaacctcg cttgcccgtt tgttcatctt gcataaggtc
     8641 agttataata atctattgct ttgtaaaatt ggtttatata tgaaccacca aataaattaa
     8701 catgaactaa taaggggtaa agttgccaaa ataaaaccct ttcatcgaag ccgaaatcat
     8761 atcctaattc gtttaaaaat tgattcagat cagatgtatt cagtggactt ccaaataatt
     8821 ctaacattgc aagatcttgt tctggatggg aataagaaat acttgggtct ataaaatatg
     8881 ccttgtcggc aaatactatg ttgccgctcc aaagatctcc gtggattagc ctcgcatctt
     8941 tttttcctag ctcccattta tgaaccaatt tcagaatttg tttttcaatt tttttcacaa
     9001 actctaaatc aaaaatcttt ttgtctaggc aaagctttaa ttgaggaaga attcttgatt
     9061 ctaaaaaaaa ggaatcaaag ctattatagt attgattgtg ttgtggtaag ggtccaataa
     9121 agttatcctc accaaacccc catttttcac tttgattgga gtaaagagtt ttgagagaat
     9181 ctattaattt atcttttttg tttcttgtat tgttatctga aatgtaatcc ataaataaaa
     9241 gagaattatt tttttctgta taaagaccat ggacggtagg aacgaaacag tttttacttt
     9301 gcaaaaagcg aagactggat acttctgata ttgccatttc ttcttgatct aaaatttttg
     9361 cgactaacgg cttgtgatta tttgtatgaa ttagatagat cggaaaacaa ctttgtgtta
     9421 gaaattgaat tttaattcca gtgcggctaa taccaataga atctaatcca ttttctatta
     9481 ttttattcat ggataactaa atcttttagc cactcgtatt gtcttttgaa aatatgtaga
     9541 ttcatctctt cttcggaaag aatatatttc tctcgtttaa aatccttatc ctctggatag
     9601 ataaagatca attgatttat attttttcct ttatgcaatt ttaaataggg cttatctatc
     9661 gtgactgacg ttccagtcaa ttcatccctc ccgataggca gtgaatctac aagtatatct
     9721 aaaacctcag cttgcgagaa aagaactgga cagaaatctt ttatgcccat tgataaatct
     9781 tgaaaaatat tcattttagt aaaatcttta attcctaaga cccgaggaaa atgattggtt
     9841 gaggttctaa tatttttaaa ttctcgtaaa agtcttataa tttcgattcc agttgtgtcc
     9901 caatccaata ctaataaatc aaaatatttt tctcgaagag cactgaaagc ataatcactg
     9961 ttattcgttg taatttcgtt aacggagaag gaatgaaaaa tcgattttag tttatgatta
    10021 aaatttttat tgtctgtcca gataagagca tggattggat agatttcttc tgtcttttta
    10081 aagtatccgc tagccatcga ttctattgag tattgatcta gattccaaaa acttgtgact
    10141 ccagaaatag aacttgctct tttttcttca tacgttagtc ttcctatgat ttgaatttga
    10201 atagaccagt tgacgcattc actcaaactt gttctatcaa aaccatctat ccatagaatt
    10261 tcatttttgt ttaatgttgg aaaagaaaaa tctttagacc aattaataac tttgcctttc
    10321 tgtaaatcaa tacaggattt taaaaatctc ttttcctctt ctgtgcagtt tgctacgata
    10381 accgctaaat gtctatcttt gaatattgat tccattaatt caatgggcag gaaagtaaat
    10441 aaggattaaa aacttgactt atataaagcc gatttttata tctaagtcct gtgctagatg
    10501 ctggaatttc agttccatca ttcgcataga tttcttttag gttatcatta ttatctagaa
    10561 caaaaatttg actcggtgat ggataatctg gtttatctac atgctttaaa aaacgaaaca
    10621 tagacttatg agctgcgata taaatatcac ctttctcatc ttcttctaga ttatcaggtc
    10681 ctgaatctag gtttattact ttaacttctt ttaaagttac agtatcccca ttctggctta
    10741 gttcatattt ataaattttt tcggcagttg tagcagctcg ataaagatat tctttgtctt
    10801 tgattttttt gtaaagtatt ccatttccgt atgttacttt gttgtcgagt aacgtccaat
    10861 tttttaagtt aaaataggta atttgagaac gtttaattcc aaagaaagca tcaaagtaag
    10921 atcgaaacat agatccagtt cctctatcat ttgaagcaaa tattctacca tctggcatga
    10981 catacaagtc attaagactt ataaaagtat cactctgtat cgtctcaaca tgttttactt
    11041 tatttttctc gatttgaaac acttcaatcg tattggtaaa tggttcaaag cttggatgag
    11101 atataacata taacattaat ttgccgttaa ttttcacaag gctcatccca tgcggatgaa
    11161 aattatttgg gtaatttgtt tcgatcaatg tcggaactga ttctgtaggt gagtttaaat
    11221 ttatttgaaa taattttcct tctaccaatg tccctcttct ttcatgactg gaaatataaa
    11281 gtataccagt ttctctatca atagctaaat cttccggacc aggcaatcca actatttttt
    11341 tacatccatc tatcgaaatg gttttaacgt ttgcataaca attcaaaaaa agagctaaaa
    11401 gtagaagtag tgaatatttc atacttctac ttttttgtga aaagccaaac attgtctagt
    11461 agaattaatt cgctttcact ttacgtttat taaatttagc tattgatact gtaaaaattc
    11521 cccatctatc tataatcata tttatctttt taaaaccatg tttcgcaaaa atttgatcta
    11581 gttccaattg ggttcttctt cgcataatcc aagaaatgcc gtctctattg ccgagagtat
    11641 ttgcaatcat ttcaagttgt gggtgataag gttgcccagt gtaaatacaa taaccgttgc
    11701 tttttactat ttggcttaaa ccttcgagtg ctttgctaac gattctattt tcagagaata
    11761 attcaaataa tccggaaaca acaactatat ccggttcaaa ttttaaattc ttgtaactat
    11821 ttgtatcaaa tgcgtctttt tttaaatacc gaatattttt tatattttga ttttctgctg
    11881 tttcttttcc ttcttgaata ttcgaatctg tgttatcaca agctaaaatt tctacctgtg
    11941 gaaattctaa tgcaatttct tgtaaatatc ttcctggacc ggaagcaata tctaaaattt
    12001 ttatttgttt tttttgtttt agaatttcta ttatagtttg ttttaaacat tcttttaaat
    12061 gtatttttct ttgccgaatt cctctccaac caattgcatc taagtatccg cgatcaatta
    12121 attttcctat cagtaaatta ccttcacttt tatttttgta aacatgatcg agcgaaagcc
    12181 cagagtcaaa accattctcg acacctatat caattccctt acttaatcta cctaacgttt
    12241 taattcctaa tcttaataga gaatagtaga atttcatgaa aatatttgga ccacttttaa
    12301 gtcgttcata ttcattttga ataaaaactt gtccgttagt cgctgcataa tccatatttg
    12361 taatattagt attaaaacaa tcctgaatga attttctagt ttcttcgatt ggtagacttt
    12421 tatttttttc gtaaaaaata ccatggtaaa aatctggata ataaacaatt tttttgattg
    12481 gtgtattgat cctttcaaaa aaaactcttt gcggttttgt tttgacgacc caatcttttc
    12541 cagcactcag tataagagtt ggggtatcaa ttgcattcgc atcttctaat attctagttg
    12601 cggtgtcatg tagctctagt aagatattta cagcaatttc ttttgtgatc attggatcat
    12661 tatcataaga ccttgagcta ttaggatcat gtgttagcat ttttgatttc acataacttg
    12721 tgatgaaggc agggtttttt attgcattta aaagtcttaa accgggaatt gcaaagggca
    12781 catacaactt gatacgaaat gcaggtgccg ctaaaatcat tgctcggata ccgggcgcat
    12841 aatcatgaac ccaggtgctg catataacgg caccaacact atttgcaacg actacaatat
    12901 tactcatttc taatttttct tctgagcaca caaatcgagc aaagctatcc aaatctttta
    12961 ccataaaatg aaacccttct gcatatccgc gtggaccagg agattttcca tgacctcttg
    13021 catcaaaaga atacatagaa aattcttcta agtttaaacc atttacaagt tcttccaatc
    13081 ttcctgaatg ttcatgacct ctatgaatta ggatcatagc tttgtttact ttttgctttt
    13141 tactattttt ccaattgcga aaaaataatt ttgttccatc aaaacttgtg aaataagatt
    13201 ctttttgaat gtaatctgac attgtatctc ctctttattg catttcaaaa tagcgtgtca
    13261 tatgaaaaaa aataggcgcg gtgaagataa ggctatcaac acgatccaaa attccaccgt
    13321 gccctggaat ggctgttccc atatctttga tcccaatatc tcgttttata aaactgatcg
    13381 ttaagtctcc taattgacca ccaattccaa caatcaatcc tgttagaata aattgaagag
    13441 ttgtaaacaa aggaaaagaa aaagatagaa gccaaggtaa aagcattgaa acgaaaaagg
    13501 atccgatcga tccctctaac gttttatttg gacttatttc tgatcgtaat ttatgtttac
    13561 caaaaatttt tccaaataca aaagcggaga catctccaat ctcaacagca aataataaat
    13621 aaaataaata tccaattgca aattttgaat ttgtaagaaa gttcaaatgg ctaaacatcc
    13681 aaccgaaata tataaatcct aaaatcgcta acgcgcaatt atgagtcatt ccctcacttc
    13741 gattgagtag agtaggaact gtcatgatgg caccaatcat ataaacgggt aaagccataa
    13801 aaagaccata ccagccagat actccaggga gtaatgggtc tggaatatag cataaaattc
    13861 ctaaggataa aattcctaag attacgagtg tatggagaga tttgttttta tttaatttag
    13921 tagccctagc aaattctaga taggccaaaa gagataatat gtttattcca actatcgttc
    13981 cggttcttcc gattaatata aataggaata taattggaac cattatgagc cagccataat
    14041 aaatcttcca tattttctga atgtctttct taaatacttt atcaattgta aaaagaatca
    14101 gaaatgctac cgttaataca aaggaaacaa ttttgagata agaaataaat attggatttt
    14161 ctaatacact taacattttt tccttcttat cattttttca tttgatacaa tttactttgc
    14221 tctaatgctt ttttaaatct gtttataata gttataatta aaagcatgag aattagagat
    14281 aggtatagat ttaagtaagg aatcgtaaga ccaaaaccag gaacaaaggc aaggatagaa
    14341 aagaagaatg ctctatcgct tttacccatt ggaccgtcat aacgtcgatt tgagttaatt
    14401 tgaattgcaa caactccagt catttcagag attattgcaa ggattgaaat tccaatgata
    14461 gtatatggat tgatttgcgg taaagataga aaggataaat agattgcagt atcagaaata
    14521 acgtccgtta tttcgttgag catagttccc aaaggtgtaa tcatattatg ctcctttgcc
    14581 atgatcccgt ctatcgcatt taaagccatt ctgagaaaaa ggaaaactgg aagggacaac
    14641 caaaggactt ttaattcagg aaaattataa agtagacctc ccataactat agaaagaatg
    14701 caggcaaaaa tagttaccat attcgctgtt atgtttaaat ttactaagag tcgagcgaca
    14761 ggtcttaata gtttttgaaa ttgacttttt aattgataga tactcatttt tgaatccttt
    14821 tttgaaattc aagcaaggag tttatatgat actttcctaa cttgatattt ctttttgctt
    14881 ggattatttg gattgattca tctatgtctt tcgcgatgcc tgcttttaat aaataagctg
    14941 ctacaatgat agaacttcgg gatagtccta acgcacaatg tataaaaaca ggtctttctt
    15001 ttatttcttt ttcgagtgta tttacaatat ggctgatttg atcttcgctt ggaggaacta
    15061 agtcaagaag tggaaaggaa tacttttgat ttttaaattc ttttgtgcaa aattcagcgg
    15121 ttaaatcaaa aacgacaatc gaatcatttg gcaattgagt tggtaaataa ccaatagaaa
    15181 gattcggtaa aatttgatca aaaggtttta atttctgtct atgataaaag tataagagat
    15241 aagcacaaac gatatatggt aagaaaacta tacaagcaga taaggtattg cagttatcgt
    15301 atttttgaag gatattcgga ccgaaagaaa tatatccaag agaaattaga aaaaaacttg
    15361 aagcaggcca taatagccag aaaaactgac cttttaatac aaaggataga ataaaaagaa
    15421 agagtgataa tgtaaaaaag aaggatgcta gtttaagagc aggctctctt ttatctaggt
    15481 tccatctcaa ccatttgatt ccaatatcag gaatgaaata agttacagca acacccagaa
    15541 aaaaacctgt gattacatca ataaaatgat gttggtaagt agtcaaaaca gaaattccaa
    15601 ttaataggca ccaaatattt agaagaagca agagaattcc ttttaggtgt attctaaatc
    15661 gcatccacaa aatccaaagc aatgcgatat gcatagaggg agcctgatta taaggtagat
    15721 caaaactaaa caaaagagta aaaagttctc cgaaaaatcc atctgttacc ggtctatcaa
    15781 atgtaaatcg aagtggccat aggagaaatc caattacaga taaaacagtg aaagttaaaa
    15841 ttcgcagtgc atgaatattt aattcttttc tactaacgca aataaaaaga gagattggat
    15901 atagtaaatc aatgctccaa taaggaatga ttgtccaagg tataaatgga attttatact
    15961 cccaagaaaa aactaaactg ccaacatcac ttctagttgc agtataggag tttgcaaagt
    16021 tataacttcc aaagaaaatg ggaactaaaa ctgccagcca tataaaagat aataaccaag
    16081 gtctcttatt ttcttgttta gaattagatt gaatcaattt tttattccta gaatcgtttt
    16141 ttgtaaaaag agaggtagga ttgatatcaa cctaacaagt aaatacattt gccatggaaa
    16201 aataattctt tcttttccag tttctaattc atttataatt tttgtagcag cttcctcaac
    16261 ggaaagaatg aagggtcttg gagatgggtt ttgtgaattt agaaattgta atctcttcgt
    16321 atcaatatag cctgggatta cttctgttac gcgaatgtta tgatctcgat atccatttcg
    16381 aaaaatttca caaatattta aaacgcctct cttcgttgcg ctatagacag tcgccttggg
    16441 ataatataag agagccgtca tagatgaaat tgcaacaaga tgtccaccgc gagctgcaag
    16501 cataacgtct ctaccagata aaaatgtatt ataagtccca aagaaattca cttgaatcat
    16561 ttgattgatt tcatccaggt tgatgatttg atctctacga gatgcataga atcctgcaac
    16621 tgcaaagatc atttcaattc tatcagagaa atccttcata gctgtttgga tttcgtttat
    16681 attttgcacg tctgctttgt agaatttaaa attaatattt gtgagtaagg attccatacc
    16741 ctcagaacct aacgttcttg caattactcc aacttttgct cccttgttta aataggcttt
    16801 cgccaaagca agacctaatc cgttgcttcc tccaacaatg agtatatttt tatttttgta
    16861 agtttctgac atgtgtcaaa cttcccttgt tcttaagagc ttctagaaag cgttccttgc
    16921 cttgtgaata atgttttaag cttgtagaaa cataatcttc atagctaagc attttgactc
    16981 ttaccttcca agaggaaact tcctcttggg ctaactcact gctaaagtca gaggcatcaa
    17041 tccagtagtc tgattcgcga attaatgagt ccgtatattt aagtcgtaag tttggatcat
    17101 aattatacac tttcttgatt gcatttgttt cgattggtgg agcaaaaggg cgactatgca
    17161 tcacaaatac ttcttctgat agtatttgtg ccaattcaat tgggtataag tttatttgtc
    17221 ctcccagata atattcatct ttgaaataag taggattgta tagatatgca tcactagtgc
    17281 tagctctcat tgctgtcacg attggaatat tgttgcgaaa taaaatttct gtatctacat
    17341 tgctttctgg atagagtttt gagataaacg agtaaacttg cagattctgt attgttttaa
    17401 gtgtttcttc atcacttata taaacttcag aaaatggttt cattttattt ttatttaccc
    17461 attcatccac attcaatcca acctctttct tattaaaatt gacgcgtcct gcgactatca
    17521 aaagaggaat ggtttgtttt tgaaagggga aacttctttg agggagataa tcaagagtat
    17581 cctctacatg gattaaattt ctctcataca cattcactaa ctcaggttta gaaaaaggta
    17641 gatttattat gattcttcca ataagcaaga tcgttttaaa aatatttgaa tcttctcctg
    17701 gtttgatcga attggaaaag gaatcataaa aatcttgtga taaaataatt tctttttgtt
    17761 tttctagatc atttgtataa ttgattacac ttccagtgat cgcaccaccg caagtagcaa
    17821 tcaataaatc tggcttttta ttttgtgcaa ttgcttcgtg atagagtcca agaaaaaaca
    17881 taggagcaaa accaagaccg ttaaaaatca taactctttg gtattttgga tttggatttc
    17941 caaacgctgg gatgcaaatt aataaactta aaaatagaaa tatttttagt tttgagtttt
    18001 tcaaaaaaaa cattaagcct ttatcctgat atacaatctc gaaaaaaaag ttaccttttc
    18061 catttaaaag aattcggaat acaaaataga tcggtaacta tggttagaaa catttaaaaa
    18121 gcaagttttt ttcgagatag atagataaaa atatcagaat agaattttta atgataaaaa
    18181 ggcattccta atggaaatat tagttttgtt ttatttatat ccaaagccga aaggaatttc
    18241 ctttttaaaa ataaaaatct tggttttatt gaaacacttc ttatatatct cgcaattccc
    18301 ctgcgcgata taccgaatgc ccctcaaata aaatccgtgt ggaaaatact tttgtcattt
    18361 ggtttatact ctagccaatg caatacaagc ttggtgtaag aatatttcaa gagtcgtttc
    18421 aaattgaaat ttttcttctg aattaaaaat aataactgag cctagaacgc gattcgagtc
    18481 ggcgattgtg aaatgataga ttctatttgc gtttaacatg gattcaatca tcatacactc
    18541 ttttcgattc aaatctccaa agtttacagt aaacaaacca tctggtagaa aaataagttt
    18601 gttgtaagtt tgagaatgta agaatctctc tttgtcaaat tcaaatctta aatctgaaag
    18661 gtcgcaattt attagttttt gaattggtgc ggcaaattgt ttgaaaccgc tggttacttc
    18721 tacttgaaat tgagaatttt ctagatagaa agaatttaca attacagctc tcggttttgt
    18781 aaatttaaat atagactcag ctataaagtg atagatttcc ttttttgaat tcagctgtaa
    18841 ataattcatc gcagactcag agagaaattc caattctgca ttgtattttt tatctatttc
    18901 atcttgcttt ctcttttctg ttatgtcttt aaatacaatg gttgcgccga tgatttcgtt
    18961 ttcttttcgg atcggagagc aagtatattc tacataaaaa aattgatcta ttttcttcca
    19021 gaaaatttca tttgtaattc gattggttgt tccttcgatt aacgtttctt taatagggct
    19081 atgctcttct tcgtggggaa ttccatccat ccagctatgg tgaaatgttt catgaaagtt
    19141 ttgattcagt aattcttctt ctgtatatcc tgttaattct aaagtataag gatttacaaa
    19201 tgtaattttt ccttctttat ctaaaccaat aattccttct tttgtatttg ttaatacatt
    19261 ttgaaataga attctctttc tttctatttc atccttttct ctagtttctt tcgttatatc
    19321 tttgatgata ataaagaatg caggttttcc gagtctttct acttttagca ttctgatttc
    19381 tactaaccta tcttctacag tgtggataac ggttgtattc cccgttagtc tgttttcaga
    19441 gggaataggc agatttactt ttgaaccaat cagaaactca gagttgaaat tcaataattt
    19501 ttgtgcattt tcattcacaa aaagcacgtg actagaaata tcagttatta tcacaccgtc
    19561 acgaatctta tccatcgttt ttaataaaat tctatcattt actagaagtt gtttcagaaa
    19621 attaatctgt aacatttgca ctgtaaatgc tcccgcacat acaatcagta aattgcagag
    19681 gatgtaataa atatctagtt tattaaatcc agctacagta ataggaacga agcaaaagaa
    19741 agtaaaaaat aaataataga ataaatgata ctttcgaata aaaccgagag aagcaaaaat
    19801 aataatcgta aaattttgaa tttgaaactc tacagaaaat tgatttacat atgaaagata
    19861 taagctgtgg agagtgacgg tatagtaggc aagatagcta acgaggcttt gcctatttcg
    19921 aattttggga ttcggtgatt cgtaaatttt gaatgcaatg agacaaagaa ttgatactat
    19981 gattcgctgc ttgggatcat cgattggagc ttttgtatat ttaaataaag cccagaaaca
    20041 aggatagatt gcagcgaata atagtaataa tttcctctgt aacatagttt acttctttaa
    20101 ctcatagtat tttgcataaa caacaggtag aacaagtaag gttagagcac tcgcagttat
    20161 aagaccacca atgacgacag tcgctagggg tctttgcacc tctgctccca tgctagtaga
    20221 tacagccatt ggaataaacc cagttgctgc aacaattgca gtcattaaaa cgggtcggag
    20281 tctaatatga gctgcactca caaccgcatc cagatgactt aagcctagat gctcttgttc
    20341 ctttgtaaac gagataagca ctagtccatt taaaacagct actccgaata aagcaataaa
    20401 accaactcct gcagaaatac taaagggaat atttcttaaa aataaaaata acactccccc
    20461 tgtaattgca aaaggaacat ttaagaaaat cataagtgcc ggagtaacct caccgaaagc
    20521 aagccagagc aaaaataaaa tcaatgcaag agtgactggc acaacaatca gtaaagtatt
    20581 ctttgcggaa atatagtttt catatttccc gccaaattca aaacgatacc cgacagggag
    20641 aggaatattc ttatgcaaga tatcatttgc ttcttggaca acactcatca tatctcttcc
    20701 tcttacattg aactctacaa tcattcttct atattgattt tgatgactta cctgaacagg
    20761 accgctctct atatatacat cggctaaatc tcccagagga atcgcatttc ctgcactcga
    20821 acgaacggga agagatcgaa tttcttctag gttgaagagc acctttttat ttgtttttat
    20881 tgctatatcg aatcttttat ttccttcgaa aacactccca actatatatc ctgaagatag
    20941 aatctcggtg acttggttta catctgtaat gcttacacca taccgaacta ggctatctct
    21001 ctttggttta attcgcaggt aattaagtcc acctagttgt tcaattttta aatctacaac
    21061 accttggatc tttctgagtt ctgccgcggt tttctcacca atctctctca gagttttaat
    21121 atcctctcca aaaattttaa ctccaatgtc ggagcggata cctgctatta gctcattcgt
    21181 tctcatttta attggttgtg aaacagatac cgctacttca ggaactgact cttctacatt
    21241 ttccacaagt gcttgcatta atttttctct ttcaaatctc cactcctctt tgggtttcag
    21301 ctgtagatat atatctgttc tatctattcc cattgggtct gtcgctatat ctggagatcc
    21361 tgttctagaa actgcatgca caagttcagg aaatttttcc ttcaaggctt tttcaattct
    21421 tctccctgtt tccattgact cagagaggga aacggacggt agtcgcgcta cctctaagag
    21481 cattgagcct tcatctaatt caggcataaa ctctgctcca agatttgcga ataatataaa
    21541 actcattaca atagaaataa gcgcaaccgt aagaactttg ttttttgcat tcatgatttt
    21601 atctagaaat ggaatataat aagttttgaa tttatgaaaa agccatgtct catgttcctc
    21661 tggttcttcc actttcaaaa tataagtcgc aagaacggga acaaccgtta gtgataatat
    21721 gaatgctcca aataaagcat aaagcacagt ttctgccatt ggtataaaca ttttaccttc
    21781 cactccagag agagataaga ctggaagata tacgatcgca atgattgttt ctccaaagat
    21841 agtagctttt cttacttcaa tggttgcttc tttaattgta ttcacttttt ccgcatctgt
    21901 taaaagggag cctttttctt tgattgccat tgaaagtcgc ctaacggcat tttctacaat
    21961 aatcacagct ccatctacaa tcagaccaaa atcaatcgct cccatcgaca ttaagtttcc
    22021 cggaacatct ctaattcgca taattacaat agcaaataac attccgagtg gaattgtaac
    22081 tgcaatcaca agccctgctc gaatacttcc caataataga aataaaacga gtatgacaaa
    22141 taaagcacct tctgctagat tctttaaaac tgttttgatg gttgtgttta ccattactga
    22201 tctatcatag taggtctcta caacaatgcc ttcgggtaaa tttttcttta gatcctctag
    22261 ctttgtatga acggcttcac tcacatggag agaattctct ttcattaaca taagagtaat
    22321 cgctccaact acttctccgc ctccatcttt tgtagttgcg cctaatcgta gttttgcgcc
    22381 ataacttaaa tctgcaatca tcgaaagtga aattggtgtt ccttctttag tctttccaat
    22441 aaaaatcttt cccaaatcat ctagggagcc aactagacca cttgctacaa ttacaaaatg
    22501 ctctttgttg tgttctatgt atccgcctcc catcgaagca ttgttctttt gcaaagcttc
    22561 aattacatcc gacattacga tttctaagga ttgcattctt tccatgttca gcacaatctg
    22621 gtattgtttt acatttccac caaatgtatt cacttctaca acacccggta ttgtttttag
    22681 aattggattg atataccaat tcaaataagt cgttagttcc attaggctat gatttcgact
    22741 gctgagtgtg aattgataga tttctccgag tccagttgaa attggaccca tcataggagt
    22801 cccataccct tttggaatat tattgactgc atcagaaagt ctttcgctca caagctgtct
    22861 tgctagaaaa atattcatgc tatctttaaa tacgatagtc actacagata atccataacg
    22921 agaaatagat ctgacttctt ctacatgcgg aattccggac atagatcttt cgactggaaa
    22981 tgttacatat tgttctattt ccaaaggaga aagggcagga gcagatgtaa ttacttggac
    23041 ttgaatggtt gtcacgtctg gaatcgcgtc catttttaat ttgcgcgcgg aatctaagcc
    23101 aaagaaaaag aaaatgatag ttgccgctaa aactatcact ctattttcga gagaccattc
    23161 tacaatgagt gttaaaaatt tcatttttac tctccaaatg tagatttgag aaatgtagat
    23221 ttaagtgtaa aaatgcctct gtctactaca tattctcctg tctgaacgcc cgatacaatt
    23281 tctactatcg acttgtttct acgacctaac tttacttctc taggtttata ctctcctggc
    23341 ttttcctcaa taaatacaaa atcttttcca tctatgtttg caatagcatc aacagaaata
    23401 gcaattacat cataatcaga aatataaagc tcagcttttc caaagagtcc tatctttgcc
    23461 atgtggtttt tatttgtgat tacaattctt ccactcactg ttctagaacc tggatcgatt
    23521 tgatttccga tataggtgag tcttccttca aattcttcct ctggataagg atttagcttt
    23581 actcttgcag tgtcgccttc tgaaactttc cctaaatctt tttcaaagag ttttaccatg
    23641 aaccaaacag aatctaaatt accgatagtc gcaagattag agattgcatc tacttgggaa
    23701 ccttttacca cttctcttgt taataccact ccatcaatag gagatagaat ttcaattcga
    23761 cctgaatttt catttccaga tccttccatc gaatttatat ctggaatcgg aattccgagt
    23821 gcatgtaaat ttcctttatc cgcttttaat tctgcttctt gaatttttaa ctcggattct
    23881 gcattaattg cttcttgctc agcggctaat tttagattta ccaattctcg aattctcttt
    23941 gcattttttt gcactgcatt gactcttgag aaagtgctga tatattttga tctagatctg
    24001 gctgcttccg gggattcaat cactgcaaga atttgaccct ttcgaactct cactccctca
    24061 atgaagcgaa cctctgtcac tctaccggag attcttgcac tgaccttagt tactcgatct
    24121 ggatctgcca taacttctcc aatgagactg attgtttctt gaaattcaac tgttcctact
    24181 tggctcgaat gtaaatttac atctttagat acttctgaat caattttaat cgaagtatct
    24241 tctttgattg aatttgtttc agttgcctct gtgttctttt tctttgaaaa atatttgtaa
    24301 gctcgatagg atgtgaaaga aattgctagt attgcaagta acgtatatat tttattatat
    24361 ttcataattt cctcatttga ctatatattg taaaagtgga aacccaatcg atctaagtaa
    24421 ttctatttta gataaatcat aatctgattg agtttgaatg taagatagtt ttaaactcac
    24481 gagtgaattt tgtgcaatca aagcatctct tatattcatt tgtcctgtag aaagtgcttt
    24541 cttgattgaa attaaatctt catctacttt tttcattaat tctggcggat aattcttatt
    24601 ttcctctttc agtgaattaa aatttgtaat cgctcgaatt acctcatact taatagtatg
    24661 ttggttgatt tctagatttg taattgcttg ttcttttcta gcttccgatt cgactacctc
    24721 tccactatta tctctccaaa ctcgaagtgg catcgatgct ctagcaccaa ccacattttc
    24781 attaaaacca tctctttgga gataagcgga aacagttagg tttggaatag actctctttt
    24841 tagtaattca attcttctct gtgctgtttg cacatcaaga actccactgt ctacttctgt
    24901 tctttgtttt agggcaagag tgattaattc ttggatattc tttgtattca actgaatatc
    24961 tgggatggtg tctataatcg agagcgccaa atcgaaatta acccccatca tcaccattag
    25021 gtttcctttt gctccatctc tttttcggat agctgagttt agaagtcttg ctgttttgga
    25081 gacttccgca ttcgcgatgt ctgcatctat agcggcagcg agtcctttgt ctgctcgggc
    25141 ttggactgcc tttgccattt cttgggaatt ttggtaaaga aattctgtaa ttttaaattc
    25201 ctctaatgaa ttcttataga aaagtgcagc ggctaacgct tgtgctgttg tatctctttc
    25261 catcgatgct actttcttta tttgagctgc gtattctgtg tcggcgactt ctattctttt
    25321 gcctctttgt ccagctagat aaaattcttg cgatgcttgg acttctccgt tgatagcagt
    25381 ctgtggaact ggatttagaa aacttacttc tgattgggct tgttttctgt aagcattcat
    25441 tacttgaatg cttggatttg atggaaataa ataagctgct gcaatttttt tccctttaat
    25501 ggctgttagt tcgattctgg tttttctaaa ttcaggacta tgagttaaaa cacattctac
    25561 tattgtgagt agtttggcat ttcctgcaca atgatcattt gattcatgag agtctgaatt
    25621 tgaatggatt ctataaattg gatagaatga tagaactaaa aaaaatatta tataaattga
    25681 tttcataaat tcctccttga agatttggaa atattaccgt cgatacgtca gatataaatt
    25741 gaaattgaat tatacgtttc tgaacaaagt agaattaaca aattgttttt atcggtggta
    25801 aatataattt aaattatatt aaactagatc taggaggtct gattagatta acaagataat
    25861 cgaaattaac cgcagtaaat aacggtgaaa cgatataaga cattgtatta tgagtaaaaa
    25921 tagtattaag ataaagattc gaataagaga ttaagatgta attacaggga caattggtgc
    25981 agacatgttt atcgtcgtga tggtcgtcct gattggagtg cggaatggag gcaaacacac
    26041 aattatcctc actcactcct attccaccgc aaatacattc gttatctatt atgcgctgag
    26101 agctaaagag tagcaaaaac gcaaacagaa acaatcgaag taaagatttc ataggtagat
    26161 ttctaggatt cttagaaatt gttagaaagg caatcttaaa attcaaaaag catttgaaag
    26221 aaagactgtt ttttatcata gggagttgct tttgattctc tattaagatg agtggaatag
    26281 agttagtaat gattgtagag agagaggaat cgaagactca ctcatcttcg caagtgcaaa
    26341 gtgagtgagt tagggtaggt tatttaattg ctttggtaaa gatcatttta ccagcagcag
    26401 tttggatgat ggaagtaacg tttactcgaa cgtctttgcc tactagatgt ccaccatttt
    26461 caattacaac cattgtgcca tcttctaggt aaccgattcc ttggttttca tctttccctt
    26521 ctttgatgac ttggattgca atttcctctc caggtagaac aactggtttt agggcatttg
    26581 caagattatt tagattgaga acttttacac cttgcagttc cgcaactttg tttagattaa
    26641 aatcgtttgt aaccagtttg gcacctgtat cacgagcaag tttgattaac tttgcatcta
    26701 cttctcgagt atcggtataa tctgtgtatg tgattttaac ttcaatcgtt ccttttctct
    26761 ggagcttatt gagcatatct aaaccacgtc ttccgcgagc tcttttgatt ggatcagagg
    26821 agtcactgat caattggatc tctcgtaata caaagttagg aagaatgagt ggtccatcaa
    26881 taaaatgggt atctgcaata tctaaaattc ttccatcgat tacgacggat gtatctagaa
    26941 ttttatctct aatttcttct ggcttttccc cacctaggat aaagggacga atgggagcac
    27001 caccaccacc aaaaattgat aatccaggct tttttgagaa ggcatagcca acttttatac
    27061 ctgcatagcc aaatccaaga tatacaagaa atcccattcc aggattcttt gactcagcaa
    27121 tgagagtcag caataaacgc ccgatcccaa aaccaacgag aagaccaatt cctacacaaa
    27181 aagcagcttc tgcatttatg ataggaaata ccttgtcctc aaaatagagg acacctaatg
    27241 ttgcaatcaa tattactact gtgtatatgg ctggttggag tatatcgcca tacatgggat
    27301 acaaaaaacc aaaagatacg acagagaatg agagcgcgaa taaaacctta tagagatgag
    27361 tcatactcaa atccttttct taaaaatttg agttcgatcg gatttattct acatcttcag
    27421 ttggttcagc ttgctgtatc atttgcggtt gaactgaact tgctaatata tcagagacaa
    27481 tgtttccagc ttcttcttgg ggaacgcctt tacttaaagc tatttccgtt tttactaaat
    27541 tataagcatt ttcgtatagc ttcctctcca tgatggacaa ttctttacca tttgcccgtc
    27601 tgaataggtt tctacaaaca tcagctactt catagataga accggattta atcttattca
    27661 tattattctg gtatctaatc ttccagtctt cctctgtgtc gacttcgtct ttcattaata
    27721 gattgagaac ttttttgatt tctctcttag gtattatgcc tcgaattcca acctgttttg
    27781 ctttatcaac aggtatcata actttcattt tcgaggattg aatttctagg ctgtagcaat
    27841 ctcgcttttt gcctaaaata gtttttttac tgacctcggt tattgcacca acgccgtgca
    27901 ttggatacac aacataatcg ccgactttaa attctatatc agaaatattc tgactcctta
    27961 tgaaatatct aaataaattc cgtttaaact cactgagaac cgtatacttt tcgatgctta
    28021 gagtcaagta aaatttctac tttggtatat aaattggaaa ataaattata ttttgcaatt
    28081 ttttttatag tgcagactgt aaaaataaaa cgagtgatgt aaagaaccat aagaagaatt
    28141 tttatagatt acccatctat caacatgtga agaagaaacg aatagaatcg agaaggattg
    28201 tctttcggat agtatccaca atgataaatt ttgtcattta ctatatctac cactataaca
    28261 aggactatac caaccactat accaagtagc cagttgttca aagcaatgtt gatctctttc
    28321 aatcctttct aaaataggga aagaatggga cctaaaaatg aaaacaattt agatgattca
    28381 taaaatccat tgtctaaaca aataccatgc aaagcaaaaa gatgattcca agtgattttg
    28441 cgatcaagag acagacctac gtgcaaaaag atctcactgc tggttcggca tttgaaagat
    28501 atattggttc cattctagag aaaatgtctt accgagtaga atatccaaat tcagaggtaa
    28561 atgctgaatc agaagcttat attcaaaatt atgcatccat tcaaatccag ttagccaaag
    28621 cattggacat tcaagcaaag gttactgaga taaagaattt atattttgaa tgtagctgga
    28681 gaagcattgg ttattttctg aaaaagaaaa cccatacagg tattcctctt tttaaatatc
    28741 aaaactactc agagaatcgt gctcccgaga cttttatcat cattggtcat ggaggaagac
    28801 cagagaatcc agagaaagtg tttatcacta ctctccaaaa attttcagat tttggtgaaa
    28861 ctattgtcta tgatttattt aaaggaaata aggctcgcca tagtcctcat caaagagcac
    28921 ttgcactcaa gcactttaaa attttggaaa gatctgatga gatggagaaa tactttagag
    28981 attcaattca gaatctctaa agcttagaga gtgattagtt tatgaattta aattcgatgt
    29041 aaaaattttt tcaccacaga tgattgaccg ataaagacgc gatggatagt tttgagtgat
    29101 cgtatctttt atcggtagta atcttataat ttttcagata cttagaactg aatattctta
    29161 atagaagctt cttcgaatac accgcctcta aatttcttca cttgctttaa aactaattta
    29221 gcatctttaa tgttgtaata atatcctaaa taaagtttgc ctgttgaggt tctaaaaagt
    29281 cttccttgga agtcttgttg atctttaatt aaactagctc cccatttgtt tgcttcttct
    29341 ctgctgaaag tcccaatttg gatgatataa ttgacttggt ttaattttgg tgggaacttt
    29401 agatctttat ggatagtagg aagcgctgtc tttgttactt tatttgtgct aacttcgcct
    29461 gtaaatccat cattgtcctg gttttcagct tttatttctt tagtgttgga agattcaact
    29521 gcttccatgg aagaatttga ttcagacgag ttcacatttc taaaagtatt gctttcattt
    29581 cccatgaaat aagactcttt ttggtctatt ttcataccaa tgactagtcc agaggtaaaa
    29641 aataaaattg ccccaataaa taaatataaa aaggaaagag gttggtggct tgaatttaaa
    29701 agatttaaaa tggagccatt attggaactc ttggatgttt taagagggat cctaggttca
    29761 gttgaatgtg atctcttatc gtctcttcgt aattttctag aataatcaac gttttgcatt
    29821 tataatttct ccacagatgt cctttattaa agttcggcga aacttacaat tttattggct
    29881 aatttatttt atattacata atatttttta tgttccaaat taaggaaatc aactgttagc
    29941 cgcttttctt agaattcgaa attatttagt tgattaaatt tatatcaacg gatagatttc
    30001 gcgacgtagg ggaaaacaga taaatgcatt gtaaaaccac tcgattttat ccgataatga
    30061 atatagtgac taatcccata caatttgaaa tggtaacacc tagaagatct ttaaaagaca
    30121 tagtctgtat ctcaatgaat gttactagag agtgtaataa atgaacgttt atctatttgt
    30181 agccgttgtt ctagctctcg gtattctagg ggtgatttat tttatattct tcggaacgaa
    30241 agcgaatatt gtagaaaaag cgttagcgct tgctgcaatg ggcaatttta tagatgccaa
    30301 ggcaaccctt cgagatcaat tagatttaga gcctaacgac cataaactcc tttatacatt
    30361 ctctaaaata tatgcaatgg aaaatgattt attaaatgaa gttagctatt tagaaaaagt
    30421 gaaaagcatt ggtaaatacg agaaagaatt tcctccattg gtggtgaata atcggattgc
    30481 aagcatctac tatcaacaag atatgtttga tgaagcattc ttttattatc tagactcatt
    30541 gaattatgat cctgtgaatc tagaagctct gattcgactg tcttttatgg ctgtaggaca
    30601 aaaggatttt gatattgctg ataaatttat gcgtcaaatc ccagacgatg aagttaaaat
    30661 tcaatcttac tttattgcaa agggtgttgt tacagcaatg ctcaatcgag ataatgattt
    30721 tgaatacttt gaaaaagcat ataagataga ttcaaattct cctgttgctg cctttttata
    30781 cgcattatca ttatctaaaa ttagaaagtt taaagatgcg ttagatatta tgcttccaat
    30841 gcaagacatg ggcggagatg atttagttcg ttttacaatc gcacaattca taatgactct
    30901 taattcttct atgagtgatt atgtatctgc acaagcgagt gcaaagacct gtatagaaat
    30961 tgcaaagaga aacaattggc atctagaaac cgctgagtgt aattcttatt atgcgatgct
    31021 ttgtatcata ttaaataatt tagaagaagc gcatgaatat ttaattgagg cagaagcgga
    31081 aagagtggat gattatgata ttattagttt ggctcagtat agatccgatt tggaagatgg
    31141 atctgcaact cctggaaaaa catccgctag agggtataat ttaaaaaatg ccttaaaaga
    31201 tttgccagaa aaattatttc cgaaagaaag atattttgaa atctctggat tgaaagcggc
    31261 agaagtgatt aatattcgtg gaataatcaa tcaagacggt caaaagatca tttctaagtt
    31321 aagtcagtta agtcccgata aaatctctaa atttaattca caaaaaggaa atgtttttaa
    31381 aaatacgtgt ataaaaattt tggctgaata tggttataaa gtgaaacgtg agttgccagc
    31441 cctcgaagca gaaggagcca actttgttac aattaaaagg gatgatgaaa gtgatcgtgc
    31501 tgtttttaga attcgtaaat ggaaaaatat gacaatatcg gatgtcttcc tgactgaact
    31561 tttgaattca atgcatgagc aacaagcaga gaagggttat gttgtaggtt cagcagaatt
    31621 aaccccaggc gctaaaaaag taattagagc aaatgaggga aaaattatgg ttgtaaatgg
    31681 aaaagactta gagagccttc tagaaagagt aattaagtaa ttgaatggaa gtatctaaat
    31741 aaaaaatttt cacaattttc caaactagtt taagccaaat cactctcaga tgattcttta
    31801 ataaaatcca atctctaaca gttttgtaat cctgtcaggg tattttttct aaaaatgaat
    31861 atctttttaa atagtaggtt ttctatgtta aaaaaaataa ttttaatctt ttatataatt
    31921 tttagtctta gccttcatgc tgaacctgat ttcgtctcta ttcatataag aaatgtttat
    31981 tccaatggca ctaaactgag tccaatggat tctaagagaa tactaagtgt tttgcgagga
    32041 ctcagagatg cagttttaga aaaggatgca gataaaattc ttcgatacat ctccaaagaa
    32101 aaaggcgctt atttagatgt aaaaggtcta tgggaatatg atcgtattct agaggaagct
    32161 aaaaaagagg attcctactt cgaggtatat ttctttgatc gtcagaaatt agtgaaacaa
    32221 aaagaatcag aagacgtttt cacagtaaga gaactcttat taaaatcaga tggaattgag
    32281 atcgatctat tttttgatag tgaaaatgct tgtgaagtta aattaatttt taagaataat
    32341 accaaattag aaaaagatat gaataatcca tacttagtga aggtgaatgg gcgttggttt
    32401 atttatcgac ttttctgaat tatataccaa ttattaggta aaaactgtca aataatacaa
    32461 gaaaaataat tcaggaattg taatacaatt gacagaaata aatattctgt gagacttgac
    32521 cctaggtata aacggatggc tgttaaaaaa gcaaaatcta tcacccaggt tagttcatct
    32581 aaacaagact ggacttttct cagtaatcat gcacatgttt tgatttgttt atacagggat
    32641 ccgactgcac gattaagaga cattgccttt aatgtaggaa ttacagaaag agcagtcatt
    32701 agtattattg aggatttaga gtcagttaaa atcgttgcaa gatccaagga cggaaggcgc
    32761 aataaatata aaatccaaga gacaatctct ctaagacatc ccctcgaagc ccacaaaaac
    32821 gtcggagact tactgaaatt attgaagtaa tccctcccgt ctttcttttc tcaacaaact
    32881 agatttttat taatgtctta atttcaaaat ccaatccaat atcaataaag tttaatctta
    32941 atttctcact atatactatc atactttcga aaaagcaatg atagagtttt ggttaatcta
    33001 acgtgagttc ggtatgagga attgacaaaa tgttcatacc atcattccct aaattccacc
    33061 ctacgttggt gctggatttg cgacccagca aatgggcgga atcgctacaa ccgacaagcg
    33121 atatctatag ttctagagac ccctgataga aaatttcggg gtgacaaaat gaaacatctt
    33181 cttgtgtcga attcacctta atctatcgtc tgcctatcgt tttataggat ttggatttgg
    33241 caaaattttt tttacaaaaa gagttacctt ttttgtttta aaaaataagg ttatgcacta
    33301 gttttataga ttttttatct tataaacaag cagtttgtat agtttaagag gtgaaataat
    33361 ttagtttaat ttataataat aggtaatatt taatacatga ataataattc atgtattaaa
    33421 atacttgaaa aaataattat gtaaattatc gttgtaaaaa agtaacttta aggagaaaag
    33481 aatgaaaaag tcaatatttt ttatgatgat ttttagtatt gtaaatagct tgcttgcgca
    33541 ggcaactgta agcccagatg aggctcttaa aagattgtta gaaggaaata aacgttttat
    33601 agcaggaaaa tcgattcgtc caaatcaaac acctgaaaaa gttaaagagc ttgctgctgg
    33661 tcaaaatcct ttcgcagtta tcgttggctg ttctgattct cgtgttccta atgaaatcat
    33721 ctttgatcaa ggtctgggag atatatttat agttcgaact gcaggtcaag tatcaaccta
    33781 tgcatcttgg ggcagtatgg aatttgctgt tacatcgtta ggcgctaaat taatccttgt
    33841 attgggtcat acaaaatgtg gagcggtaaa tgcagcatgt gctgttcctg atgtgccagg
    33901 gcatattgtt accttaatca atgcaattaa acctgcagct gagattgcaa aggaaatgga
    33961 aggtgactta ctagacaatg cagtaaaagt aaatgttgca aagcaagtgg aacagttaag
    34021 agctttagag cctgttcttg caaagaaagt caaaagtggt gaaatcaaaa ttgtaggtgc
    34081 agtatatcag cttgaaacag gaaaagttga gttagttcct gacaattttc taaagaaaat
    34141 ttctaaggag aagaagtaat ggagatattt agttctttat ttgctagttt gctaacacct
    34201 atggtgttag cgttcgtttt aggaatattt gcaactctga ttaagagtga tctcaaattt
    34261 ccagaaggtt tgtatatagg tctaacgatc tatctactct tcgccattgg tttaaaaggt
    34321 ggaatgaagt tgtctgctac ctctctagct gatttttata aaccagcttt ggctgcaatc
    34381 gttatgtgtt gccttattcc agtatggtcg tatttcattc ttaataaact tggtaagttc
    34441 aatatttcca atgcagcggc aattgctgca cattatggat ctgtatcggc tgtaacattt
    34501 agtgaaagtc ttgcttttat ggaagtaatg aacgttagtt atgaagggtt tatgcctaca
    34561 atgcttgcaa ttatggaagt ccctgcaatt ttaatcgcaa tctttatagc tagaaccaaa
    34621 gaaggtaata atgattcctc atggggcaag ctctttcatg agttatttgc aggaaaagga
    34681 actgtattac tcattggtgg tctcataata ggcttgctaa ccggaaaaaa aggatatgag
    34741 caagtggcac cattatttga tactcccttc cgaggaatac tcgctctctt cttacttgaa
    34801 gttggattgg taacaggaaa gaggttaggc gacttagtaa aagctggtcc attcttaatc
    34861 ggatttggtt taatcatgcc agtgattcat gctacacttg gaatcttgtt tggtaaatat
    34921 gttggtttat ctatgggtgg agcaacaatc tttggagtct tatgtgcaag tgcttcctat
    34981 atcgcggctc ctgctgcaat tagaattgca cttccagaag caagcccaac gtattatcta
    35041 actgcatctc ttgctataac gtttccattt aatatagtat ttgggttgcc tctttatctc
    35101 acaattgcaa agaatgtttt cggagtgaac taatgaatct acatcctata caacttataa
    35161 caatcatatc tctagatgtc ttagaagata aaataatcaa tgatttaaaa caacttggag
    35221 tcaaaggtta cactgtaagt gaagctcatg gcgaaggtct tagcatgaga agagatgaca
    35281 attgggaagg cagaaatatt agaattgaga tattagcgaa ttccgaattg acagaaaaaa
    35341 tctttggact ccttcaaaaa gagtatttcc ctaaatataa aatgatcgct ttcgcacaag
    35401 aagctaaagt tctccgtaaa gaaaaattta actaatagac ctaatttgga aatacttgat
    35461 tcattcaagt atttcctttt tttttactcc aaatcccttt tgaacttttc ttggttttca
    35521 tagcaagtaa aatcaggttt actgataaag tcgtaagaat tacactaagg attaatatcc
    35581 caaatgctat tggctttaat aaaatgagtt tttctttttc tgaagcttta agcgttataa
    35641 tactataacc tggatttttc atcgggaaag ataattctga cttccacaaa tcctgctctt
    35701 gattgtatga aacaatttta aacaaagaaa tatcttcatt aactacaatg tatgtttcat
    35761 tgatgttaga gtgattcgct agaggatttt gaattacact ttcttctttt ttttgattat
    35821 tatctgttaa attctttttg cttaggaaag aaattctttc attcagtatt ggatggttag
    35881 gccaaatccc aagaacttgt ttgtataaat catttgcggt tttataattt tcttttttat
    35941 aatgagattc cgcttgctga aaccatgtgt ccgcctgtag atatagtaac tcaggatctg
    36001 atggattatc ggaaagaaga gaattaattt ttctttgtga tgaaataaaa tcttttcttt
    36061 ctagaaaaga ttttatttct gaatactcag ttctttcttt cgaaataaga gattgttgaa
    36121 acagcaatat cgcaaataac acaaatttag cgtaaatcat aggacggtca ttgagcggag
    36181 tcgaactgac gatactccta ctggttttat tagttggcat cttgacctca tttaatgaca
    36241 gaatatagaa taatcctttc tttcatctat ttaaaattac cgctttatgt gcatatcgtg
    36301 ctattggcat tttcgttgta gctatagtcc attgtccacc aactggattt gccatatcca
    36361 aatagtaaaa ggaatcattg gctactccag aagatagtaa tcctccaaat gtataagccc
    36421 ttcttttgga ataagaaaat tcaactgctg gataatataa gccaacgggt agagcaggac
    36481 cagatgttgt tgtgttcgga ttcacaccag aactatttgg tagataataa tcataagccg
    36541 ctgaaacgat ataactggta acaggttgtg cagtattgag caaagtgctt cctccaacta
    36601 ttaacacagc tggtgaatct gatggaaaag agtcagtgga ttttggaatg taacaagccg
    36661 aacctgctcc atgccttcct acattcagag cagtttctgt tatacctgta gttgagttgg
    36721 aagaaaaaat atagccatcg ttagtcgact gaactgatcc atcagaaaaa agtcttcctg
    36781 aattaaaata tagaactcca ttgatagaac accctcccat atctaccttc tgagaaatag
    36841 cacttgatga aactaaagtt gaccaagttc cagttgctcc aatatcggga ttaaatttat
    36901 aaactgtatt tagtaatgtt ccagaagcca tattaattgt agttgttccg gctatcgtat
    36961 aaatactgtt acctcctact ccaataacaa agccttgtag agatgttggc atattcgaca
    37021 tggttcgcca tgtttttgta gatggattaa attcttctac tagattagtg gctgcaattt
    37081 gattcgatga atgtaaaaac aatccaccta ttacataaat tttgcctcca taagacacaa
    37141 ttcctgcatg acttctagga gtaggaatat ttgttatttg aggataccat agatcatcca
    37201 caggatcata aacatctact tgtgatataa aggagttact tgttgttagt ccgcctagaa
    37261 tccaaatacc gcgattataa atttcatcca ttgatggggg tgatggtgga ggcgtttctt
    37321 gttttaatgt tttgaacgtt aataaaaaat ttaatggtaa aaaggtttgt aaagtatttc
    37381 cacaaaatgc taaatattta tatgttgtat cttgtgtaag attggataga ttaaatctgt
    37441 ggagtttaga tttaaacgga gtataaagag tattattcgc aatatttttt ccgtagagta
    37501 atctcccctc cgcttcttgt gaacaagtcc aagaaataat agcagaggta gtcgtgattt
    37561 cattgacata ggtaatttcg agcttcaaag tttcggtctc gttaccttta ttgacgggac
    37621 tgttgctgca atgcaaataa aataagaaag ctatgcagat agaaagccac tggagaaaag
    37681 aaatgtcagt tcgagcagca cagctttttc gtgcgtcgtc gagaactgct agaaaagaaa
    37741 agccctgttt tacttgtagt tctcgactaa cgcacacttg gatgccgcta ctcgaactga
    37801 caattttttc caaagtcata attgtgctcc aatagaaatt gttccgccac caaatccagg
    37861 accgcctcca ttctcataga gataaattcc tattatcccc aaacgaataa ttacttttcc
    37921 aaaacttaaa cctccccagc ctacttctaa ataaccagcc ggatcagagc ccgtaactgt
    37981 tttatctgta tatttataat taacaatcga tcctccaata cctaagtttg cgtaaaaatc
    38041 aatccagttc gactttttcc aattgtattg caaataaaga aaattattat agaaacggaa
    38101 tctcgaattt atactttcca agtattctaa tttataacct agccgccatc tagaattaaa
    38161 atgcacaaaa gaaggctcga tgaaaagccc tccaccggaa gcttggttca cattattaga
    38221 agattgagca aatgcagaat aagaaccagt aagtcctata ttcgaattct tctcaggcgg
    38281 caaatcgaca ttatacttat ctggaattaa actttttgca ttttccaatt ttgatttttc
    38341 aatcgtaagt aaaccaagtt ccgtttttac aattaggtca tttgtatttt gccgaacgat
    38401 aacgcctttg atttgagttc catcttttag aatgattgta tcataatgat aagaaatatg
    38461 ttctcctttc ttttccgtgt cgatattctg tatttccgat ttcggaatat caaatgtttt
    38521 ttctttccaa cgaattctga ctttgtttgc atcattctct aaaatatctg caatgaatgc
    38581 atcaccgttt tttaaaacaa tttctgctgc tgaaagagaa atggaggata ggagtaagaa
    38641 agaaattatt tttattctag ctgacaattt cattttgatg tttaaatgtc cttattgcaa
    38701 acctgtgtca gttcgagtgt aatcgagaac tacaagtgct aaatgccttt attttattag
    38761 ttgttctcga ctaacgcaca cttggatgcc gctgctcgaa ctgacagtta ctataactct
    38821 ttcacaaaac ttgataattc tttagagact aactttggca atcctctaaa ggcaagtccc
    38881 ataccgtttt catcaactcg cacaacgcct gcctgcaaat cataaacgtt attttctaaa
    38941 gcaaacttca cattaaccgc ctgatttaac tcataagggc aatcagacca gagagcgtat
    39001 actccggtag agcttacgtc tgttgttttc aactcttttc catttactga aattttcatc
    39061 tcaattggtt ttctcttttg taaacgccag cctctaggat acatcttgaa gtaaggagca
    39121 gaaatatctt ttcttacgaa ataggcaatc gaagcaaatc caaggatgct attaaataag
    39181 gcagcaaagt tatagagttt aaaattaaaa acaaagactt gtaggttata aagaattaaa
    39241 agactagcgt aaactaaaaa caactgccaa ccccagcgct taacattgat tatccctatt
    39301 ccaacgggaa tcggaagaag cattaaaaaa agagcgaata aatcagaatg aaaaaggata
    39361 attctaaaat tctgtgggga tactccaagt tgcctgctga gcaaatagta atttaaaaaa
    39421 ggcaatatta gaaatatgat tccgaagatg accaatgttt tcggtcttga aagacgcttg
    39481 cgtttttgat tcatgaattt agtccttttt aaaattgaat tgtttggagt aagatttgct
    39541 tcgtgctatc cttacactca ttaaatttgg gtagcgcaaa attacaggta taggaatttc
    39601 ctaatggcat tttcactggc ttgctagtaa agcccaaatc cagacaagac ttcccgctac
    39661 ttttgtattt tttagtcagg ctatttccct ctgccatctt cgcgcaccac ttcgacaaat
    39721 taggattatc cgactcaata caggcaaagc tatttgcttc cttagcggat ggctctcgtt
    39781 ctccgctcac gcaggattta aagacttcct gctctgcgga aatgcttatt gttgcaatgg
    39841 tgaaaaggct cattagaatt agtttgttca taaatttatt ctccttttta aaattttgcg
    39901 tttagaccga tataccaatt gttgatgtgg tcggtgctgt gaatatagct accgtaagaa
    39961 gggtaattga tgatatcata gtatttggta taatttatcc gtcgatcatt ctcgtatcga
    40021 atttttgtat aaataaatcc ataccccaca agaaggttta aattttcata gatattaaaa
    40081 gataaattga tatcgaattc cgctccatag aaatatattt ttgcatgtgg atcgttatac
    40141 gtagctcgga aatcatttgc acttggtatg ggacttccgt aaggttggat aatatcgcgc
    40201 ttaatatttc ttgctccata agtgtaaaaa ggttcaattg aaaaattaat agatagattc
    40261 gaaattattt tgtattgata tttcaaaaat aaatttattc catatgtatt gtctagaatt
    40321 gaatcttgtg taatatatgt tctccgatag ttttcctttt ttatatttcg agcaccaagc
    40381 ccataaaaaa agaattactc tcatttagtt ttatatttga atatacattc aatttatttt
    40441 cacttctcct tatattccca atgtcatgcg gtgtaattga gctaccgaaa agatctgcaa
    40501 cgtaataagg actatctaaa actaagctat aacgagagaa ttcaaagttc aaattcagat
    40561 tcttgttata atataataag gaaaaaggat ttattacctg ttcattattt ctcatagggc
    40621 ttaacttctt aggattcgcg gcactaaata gttcactttg tgaacgtagc ccaggtctat
    40681 cccataaccc caaaagagtt tttcctattg agtcgttctc atattctact ggataatacg
    40741 cataattcgc tcttttgatt gtaaatccga attggtgtat tttttcactc agcttatttt
    40801 ccttattatt atctatattt ttttcttctt gagaaaaaat gtttgtatta attacaatta
    40861 gaaccaatat gagaaaaaaa ccgcgagtaa gatgtttttt gaaatctgtt tttttctgaa
    40921 tatctttttt caatgtcatc tcctgttaac ctcacatccc aagtaataag caagcagcat
    40981 actgtgcagc ctgatccgta ctatctagaa caattacttt cacatccatt tgcccaactg
    41041 aatcgactac tagcactgcg aatccccaaa ctgcattcgg cataaagtta ttcgtacccg
    41101 gaccagttaa aagtccatct gcattcatag aagcgccagg cggaaggcta ccactcggaa
    41161 gattagcctc cggaggcgca tttgtggctg tataccattt atagggaggc acaccgccgt
    41221 taaccgcaaa ttgaatagag tatgcgttct gccaagaaat tttacatccc ccgctacagg
    41281 tagtaaagct tggagtttta gatgttccat tttttattac atgatacatc aaaggaacgg
    41341 cgactggatt atcatttata tctaatacac tgattgttaa tgtctttgct gctccaagac
    41401 tccatttgga actagcggct aatggcaacg tatcattata atcaatcgtc cgtccgaatc
    41461 taaagttagg gttgagttct gaattcatag agccagagaa cgtgatacta tcggatgcag
    41521 ataatgtttg aataaagaac aatagaatta atactgattt cataatttca ctcttgcttt
    41581 aaaattctgc tttaatacca acgtaatagt tttgtatgtt atcatctgtc ttttcataac
    41641 tatctcgttg gttaaaatag cggagaccat tactatcaaa gatatagtat gaatttgaaa
    41701 cattatcttg gtatctattc tggactcttg ttgcagttct tatataatta tatccaaaaa
    41761 agaaagacaa gttgtcgtct attctatacg atagatttat atcagcatca taaccccaaa
    41821 aataaacgaa cgagtcatta actattggaa ataaataaac ttttttatta atcgaattat
    41881 tatatggaaa atctttggta atgtatttgc ccgcagtata aaaaggctca aaagtaaggt
    41941 tgaaagcgaa cgaagacaaa aaatccaatc tatactttaa aaatatttcc attccgtatg
    42001 tatcagtgcg gatatatctt tcatttgagt aaagattact cgacacttct ttgaagatat
    42061 ttctaatacc tagaccaaag aaaaaatgat tacggtcatt gactgaatat tttttatatg
    42121 catttaattt gttttccctt ctcgtaatag ttggtagtgt atatcccaaa aaaaatggtc
    42181 tcaattgtcc gggtgaactt tctaaaaatt ccgttttagt atagaacaaa ttattgagac
    42241 taattttgta ataactgtat tcaaagaata aattcaaatc ctttgaataa taagaaaatg
    42301 ataaagggtt ggtctttgtt ctattgttat gaagaaagct tgctgaattg gatccgagag
    42361 tatttctttc gcggtaatct aaatattctg taggtaaata gctaaaatgc gcactcttaa
    42421 tagttagaga atatttcttt tgcgtattca attctttatt ctgctcttct gaaaaaataa
    42481 cttgagagca aaataaaatt gtgagtataa ttaacttttt cattgttttt ccttatacgg
    42541 gcagctttat gtttatgggc aaaagcctgt cagtaagcat gcagcatact gagcaactaa
    42601 atctgtagtg tcaataacta caacggccgg gtaagtttgc cctattgaat caacaaccaa
    42661 aataccaaag taatatgtcg cgtaaaaatt atttgtagca ggaccggata ataaaccttc
    42721 cctgctcact gatgcccctg gcggtagacc cggaccgaag cctggcggaa gagatacagt
    42781 taatgctgta gggatatcat taattgtata ccatttataa ggtggcacac caccgtttgc
    42841 ctccagttga attgcatacg cactctgcca agaaagttta cactctggcg agacacaggt
    42901 ataaaaggaa ggaagcttcg atgtcccatc tttaataata tgataaaaga gactgataga
    42961 aactggttta tcatcgatat cgaatagact caaattcaaa gaacgaaaag aacctaaact
    43021 ccactttgaa ctcgatttga tttgcaaagt atcgttaaag tcggttgttc tagtaaattt
    43081 gtaatttgga tctattagca aactcaaatc cccggaaaat tgcaagttat cagatttaac
    43141 tgtattctga aaatatattg ttagcatttg ctgagattca aaaataactt gagcattttg
    43201 gggatcagtt gatttaactc ctacgggtgg tgagcctgaa agtaaatcat atccctggag
    43261 gtattgattt tcctgaatac ttttacgacc attcgtgcaa gccgactgaa atacttgttt
    43321 tggctcaccg tatgcaacgc cacatttatt tgcttctcca tcgaacttat ctaatgttat
    43381 ccaatcgtac gttgttccac ctggaatttc attgcctgct gaatcatatt gtcgataccc
    43441 tctaattttc ccaggagtgc aatttaaatc ggtaaattta gcacctatga gaactgttgt
    43501 gcaaggttta gcttcgcata agcacggcac tgaagaggcg ggtggtttag gacaaagtgg
    43561 attatgctgt attccatcgg aacaatcatc agcccattgt tttagaatcc caacaacaaa
    43621 ttcgattagc ttatcttttt tctcttttga gcaacctgaa caaccagcgt tactgaaagt
    43681 aacaaaaaaa attgttaaaa ataaaataaa taaaaatttc tttaatttca tttctgtatc
    43741 ctttgcttta aagataattt tgcttttgta catttaaaaa ttgttctact ccaggatatt
    43801 tgaaaagatc cttggatgga tcataattat ctggattaat tgactcactc atgaaattta
    43861 gaacagcatc aaaacttggt ggtatatatc gccctaaatc ctccctatca aatatacttg
    43921 tctcactaaa agtatgaatg gcatagagat tataagcatg aattagcctg ctattaggat
    43981 gcatttgcaa acctactaca ttttttaaac gataaaatgg aatcggaaca gaaggaaatt
    44041 cccaaatccc gctgtgcact tttaaagttg ggtcaaagga aattaatgcg ttgagcgtgg
    44101 gggtatacgc atagtatcta ccttcaaaag agcggacaat ggacgttacc tgcaagggtg
    44161 cagtaagatt attcaggtct ccatctatgg ctatttgaat caagttcgta ttcgcttttt
    44221 cttcaaaacg cgaattagaa tctgctccag tatctaaaaa cgcaataatg gaagtgcctc
    44281 cgttgtagat aatcaagatt acgtcctctc ctgttgagac agatattgga ttcggaagtg
    44341 gttttctaaa atctaacgct ttaatatctc cagttactga gtggatgctt gaaaaatttc
    44401 catttgcatc aaagctaata aaactatctt ttaaatgagg agctatttta acagaggaga
    44461 gtgcggttgt attacaagaa ggatttctaa taacggaatt tccatcaaac gaatagcaga
    44521 gattgatatt tccatttgca tttagtattg caatctttcc gttcacttga acagaaattt
    44581 gtgtcgctcc ttttaattct ggattttgcg tataatctaa ttgctgtaag cctctagtga
    44641 agaggataag acccattttc ccatctaaga taaattgatt aaagtcccaa tctaactgtt
    44701 ccgatttttc taaatcttta tttaacttaa ctacagagac atctgtgatt tggcgcactt
    44761 ctgggaagtt tggatttcta tgataaaaaa ttccgagtaa atccaataaa ttcaaattgg
    44821 tttggtatcc attcattata gttggttgct ccattacact cacaggaact cctactatgg
    44881 ttggagtaga tcctgtcgga aggctaccat acggaggaga accagaggct gacgatgttt
    44941 gactctgtgg gctactagtt agtaatcccg ccaaggagag ataggaagaa gaatcagaac
    45001 tctttgagcc aatacagaat tgactcatta tggaaataca aataattaga aatattcttt
    45061 ttgaatatag aatcatagac atacctttta aattttattt ttacgtgctc taatacttac
    45121 atacaactca ccgaccaccg cttaggtgtt aggtgttagg tgttaggtgt taggtgttag
    45181 gtgttaggtg ttaggtgtta ggtgttag
//
LOCUS       DAOQOS010000038        43915 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_338_length_43915_cov_5.907615, whole genome shotgun sequence.
ACCESSION   DAOQOS010000038 DAOQOS010000000
VERSION     DAOQOS010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 43915)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 43915)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43915
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_338_length_43915_cov_5.907615"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(66..461)
                     /gene="rbfA"
                     /locus_tag="PLF66_10245"
     CDS             complement(66..461)
                     /gene="rbfA"
                     /locus_tag="PLF66_10245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001068309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HMZ64465.1"
                     /translation="MNETRKKKIESEIIKSVANLIVSGKLKDPRIGIVSVHRAELSND
                     MTNVKIWVTSYTDDKGKKTLLNALRSARGYFQHILAEQLKLRLTPKILFLWDEDYIKS
                     LEMNQFLDNLPPIRNYQEESEAKEEDTEN"
     gene            complement(486..3092)
                     /gene="infB"
                     /locus_tag="PLF66_10250"
     CDS             complement(486..3092)
                     /gene="infB"
                     /locus_tag="PLF66_10250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015681770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HMZ64466.1"
                     /translation="MEEKKDNKSIKDKLLQGQDSSHKKIVIKRKSPIAETPATNVNPP
                     KPKKDLDLLVKEETERQQAAPKPFMKTVDDSKIIVNRNVSETFQQKEKEKEKDVSETQ
                     TQQQSKETNVHQETRKQNINQQQSNTSQQSSRPHPAYPVRSADRNPIVSRPQKPVGGQ
                     QSNRDNSSQGSGNRPNRPQGNYQGQGGGNRNFHQKHPRPNQGGGNQGQGNYQRRPSNQ
                     GQGGGSFNPIEIPKETTTPGITVNRKRNNQGTFDKNKDKNAERAQENSKFFKQMYKKT
                     SGVPVSGTTVPKEISIMENVQVGELAKKLNLKPNDVIAKLMKMGMMVTINNVIDSDTA
                     ILLADEYGCAVKIVSLYEETVIQEEKDSPDDYINRPPVVTIMGHVDHGKTKLLDTIRK
                     SAVIDTESGGITQHIGAYQVHTPRGVITFLDTPGHEAFTSMRARGASITDIVVLVVAA
                     DDGVKPQTIEAVNHAKAAEVPIIVAINKIDLPSANVERVLQDITNLELLPEEWGGKTI
                     VCKISARENIGIDKLLEMILIQAEMLDLKANPNRVAKGTIIEAKLDPGRGAVATVLIQ
                     NGTLRIGDAFLAGVHSGRVRAMYNDHGHHVQMAEPSFPVLVTGLDAVPDAGDPFDVIR
                     DEKEARNISQHRKEYQRIGQAATVTKVTLDNMNEIISQGELKELKIIIKTDVRGSAEA
                     IKESLEKLSTGDVKLNVIHAGTGAIVDTDVMLASASNALIVGFHVRANPRTLALAEKE
                     GVQIKYYSIIYDVVNEIKAAMEGLLEPEQVEKSNGKLEVRNIFKISKVGNIAGCMVTS
                     GKIHKNNLIRIWRDNVVIFEGKLKSLKRMKDDVTEVLTGFECGLLVDGFNDFIEGDEI
                     ESYEINSVARKL"
     gene            complement(3131..4516)
                     /gene="nusA"
                     /locus_tag="PLF66_10255"
     CDS             complement(3131..4516)
                     /gene="nusA"
                     /locus_tag="PLF66_10255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004449562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HMZ64467.1"
                     /translation="MATKQVNNESNLFEAIQQFCADKGLSKDSVLSIIKDSLLGAYKK
                     KLGLDSTKVNNINVEFSDKNEVVIVISKTVVEAPSDSPLEISLDKAKQVDPDATIGST
                     IAFKEKPVELSRIVSNQARQMVFQKLKEMERELLYNEYKVKEGELTHGYFQRWINKDT
                     MSIDLGKVEGVMPKKEQNPGEKYKSGDRLKAIISKVELKKDKFRDTGLVITLSRASGD
                     FVKKLFEMEIPEIYDGLVQIVDIARLPSVRTKIVVRGTRSDIDPVGACVGMKGVRIQS
                     IVRELGNERIDIIQYSDDPSEFITNAISPAKPVEVRVDHENREALVVVPDDSLSLAIG
                     ASGSNVKLAVQLCQFKIDIKSVSQYNEDMTSPEARERLDRLFNNHKANHSKATESTED
                     STRGTPLSEIPGLTPRVITLLESGGVRDIETLVEMQPDDLAKIPGVGPTTSEQILRLL
                     SESVEFVEEEG"
     gene            complement(4518..5000)
                     /gene="rimP"
                     /locus_tag="PLF66_10260"
     CDS             complement(4518..5000)
                     /gene="rimP"
                     /locus_tag="PLF66_10260"
                     /inference="COORDINATES: protein motif:HMM:NF011228.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HMZ64468.1"
                     /translation="MALQESEIREALATIIEHPIQLYSLILQFANGHFLIEINLDNLQ
                     DPRGSVSITDCEKVSRRLTDFLEATHGQENYTIQVSSAGAERELKLPEDLGRFHNVPV
                     KIFYLEKGKKVNNVFQIMGFDGTQVTLEPLTEKSKKSLGNRVVLDKNDILKGNLYIKF
                     "
     gene            5485..6507
                     /locus_tag="PLF66_10265"
     CDS             5485..6507
                     /locus_tag="PLF66_10265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64469.1"
                     /translation="MLKTLFFLLILTHSIYTEETIQVEGFTQEAILSEEEISFAGEKT
                     VFIKVTANGYLSSGTEDSYLEVIGKKISEKKQRVFLLLPSGYEPKIWVKESESIFPKH
                     EIKLIRRYLHDLCFDRRQLIGSEADYSYYSKLTEECYVDKTRDYLKGNRFQYAVYRYF
                     GRIPEWTDPFDFILGFFLYILLFLSSLPHLLLFLFIYSFFKEYFRKEEYKKKLMTVLF
                     PFIYGFLWVFGSIYIGETFRFNFWGMGVWKFYFYISGLFFGLGFLGIKSVVNRVFHPE
                     EDVLPDWAIGLILFFSPLLIFAAGAGGGKQRSSSSNQNTNSSNSQSTSGGGGTFGGGG
                     ASGGYS"
     gene            complement(6635..7057)
                     /locus_tag="PLF66_10270"
     CDS             complement(6635..7057)
                     /locus_tag="PLF66_10270"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HMZ64470.1"
                     /translation="MIIYLDTSAFLRWLLNSPKVYSEFQNWQACYTSELLYIEVSRVL
                     NRLRLEKEIDDSEYADLHKTFLEFYKTIYVIELNQAVKQKSAEPFPTVIGTLDAIHLA
                     SAILLKEENPKWQIQFLTHDSQLSTAATAMGFEVIGIH"
     gene            complement(7054..7308)
                     /locus_tag="PLF66_10275"
     CDS             complement(7054..7308)
                     /locus_tag="PLF66_10275"
                     /inference="COORDINATES: protein motif:HMM:NF014643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HMZ64471.1"
                     /translation="METISISNLKTHLSATLKEVVNGIRYVVMDRDHPVAELIPYSKK
                     SRLVVQAPVSKTKKVKNTFKGKVKLDPVEYVLQDRRGGRF"
     gene            7699..8304
                     /locus_tag="PLF66_10280"
     CDS             7699..8304
                     /locus_tag="PLF66_10280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64472.1"
                     /translation="MKIIILLLLLLSFSFCSSKTEKPIAERTLDGWAGHPNDPNKKPF
                     EYYYMTSPGKASPKSIQRKSGPMAQSTCTDNALLGMSGDLIGKMIGDIAEAGSPASGF
                     LLPPIQLKKQLTKSRENFEFIDLSILVGFPTEEESSNGNKKERRPIPIESRGFKECKP
                     TATPIPDFPFSEWRECACLVYAHVKGGKETIVKYYKETYLK"
     gene            complement(8306..8875)
                     /locus_tag="PLF66_10285"
     CDS             complement(8306..8875)
                     /locus_tag="PLF66_10285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64473.1"
                     /translation="MKSKQINFYLMPEDISEIDKYLQEKDLSVYNNYSTNSNLQKLDS
                     LLQTKILGKISSIKYVTSGDFIEQINNQFIERQNYYSIDAISSPVIEFLIPKNIISGN
                     IIHSGRIYYIHSYYNSQNLLVSKPDAFLKMSSELFKWIKKNFKNVKLPGFESYLISER
                     TEVWMKTTGGKLSDMVREEEFRKKSALAV"
     gene            complement(8881..9207)
                     /locus_tag="PLF66_10290"
     CDS             complement(8881..9207)
                     /locus_tag="PLF66_10290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64474.1"
                     /translation="MSNDKNIPNPYGRKGGIEHQAKVKEVATDIKERGFLAVFEKFLR
                     LFNGKGKFMDVVAIEEENNQIVEIHQVGKQNQDGRPVKRERDTIEEVTQNYGIKPMFH
                     SYNKKD"
     gene            9330..9944
                     /locus_tag="PLF66_10295"
     CDS             9330..9944
                     /locus_tag="PLF66_10295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64475.1"
                     /translation="MKIIILLLLLLSFSFCSSKTEKPIPERTIDGWAGHPDDPNKKPF
                     EYYYMKHASTASQKSIKSKNGDLIRSTCIEANKSQAKTNLIPILIGNTIFSQSSPGGQ
                     LPIDSSLKYLHCNNDNFYFNVRIDPFNLRNIIALEIWELEKLLIKEYKKIIKVVEVKD
                     CKPLAVPDPEIPLSKWKDCECLVYAQVPGGRDAFIVRAQKIDTD"
     gene            complement(10320..11168)
                     /locus_tag="PLF66_10300"
     CDS             complement(10320..11168)
                     /locus_tag="PLF66_10300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010419457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HMZ64476.1"
                     /translation="MIINHNVSAINSHRVLKFNNEALNKDVEKLSSGMRINRAGDDAS
                     GLAVSEKMRSQVNGLRRAEMNAEDGISLIQTTEGWLQETQEIVQRIRVLAVQAANGIY
                     TEEDRQQIQVEVSQLIDEIDRIASQAEFNKMKLMTGAFSRLNPTASMWFHIGANMHQR
                     ERVYIETMTTAALGLRNPTVLTFISLSTLSKANSVIGLADDALKMISKQRADLGAYQN
                     RLEHAAKGLMNGYENIQAAESRIRDTDMAEQMTSLTRYQILTQAGTAMLAQANQKPQN
                     VLQLLR"
     gene            complement(11328..11401)
                     /locus_tag="PLF66_10305"
     tRNA            complement(11328..11401)
                     /locus_tag="PLF66_10305"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11365..11367),aa:Asp,seq:gtc)
     gene            11500..11871
                     /locus_tag="PLF66_10310"
     CDS             11500..11871
                     /locus_tag="PLF66_10310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64477.1"
                     /translation="MKKIFLLLLALSLVFSADCRELMTKKNICHLSVQKAKTSKADCH
                     GNKEKTKNSCKCPETKATQLQDTSLKESRKVVKLELLFTSPINLVHPISILTKTDSAY
                     LLNLSYFYTFFHPTETTHLLI"
     gene            12084..13418
                     /locus_tag="PLF66_10315"
     CDS             12084..13418
                     /locus_tag="PLF66_10315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002979337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HMZ64478.1"
                     /translation="MKRTLYIILIFLLTLGLYSEDIEIQLENLISLHPEIKSLEAELK
                     SKKANAGHETTYPDPKIGIAFRNYPARSYSLNDKAYDTPSMTGIEYSISQEIPFPGRL
                     TLQGKVSKLGELEFLHLSKSRKNQFLLEYLTQLVRLKSAEKKIVLNELIRKTLESQKT
                     ISTSTLAVGGSNLSKSLKLQVEASLSQEREIEYTKDKKNAIAALEYFKVDTSSKDLLI
                     KLDPSKYLKEKAIQIKSEKNILKENLIENPNYKLSSVAIERAKNESKLSSLLHAPDTE
                     VFFAYMKRRNQIITLDNGPLNYQIMDNTEYRGDLFSFGVNVRVPVWSFFSSHDLKQRS
                     EENVQSKAFELERNKLFLESNFEKLLVTLDSIDLQIDLYEKRIIPELNKSLSALSSQY
                     GTGKVDLIEVLNSKIELQRAFITEEELQEKKKITTLLLLELTDHLLKPKEGN"
     gene            13420..14469
                     /locus_tag="PLF66_10320"
     CDS             13420..14469
                     /locus_tag="PLF66_10320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002979316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HMZ64479.1"
                     /translation="MKTLLLYIVLSFTLIGFFESCKKEKEIWYCPMHPTYTSDRAGQC
                     PICNMTLVKKEILPAKSTSHSEHDHENMPASTGDKKEENTIHLPEEKQRLIGIKTARV
                     ELIPLSKKIVAYSRVAYDPELYTAILEYREAKKASQILTDDSLPNSSSLANASIVRLK
                     QLGLSDSQISEWGNSNRNPDELILGSKNGRAYIYSSIYETDIGYVKKGLKVSLKIDSF
                     PDKIFTGTIQSIDSILDEQNRTLRFRSYVSDRDNILKPQMFGNIEISIPLKKALSIPK
                     SALLNTGKHKLAYKRVSIEEFTPVMVKTGMETDDYYEVLEGLEEGNEIVVESNFLLDS
                     ESKIKLGGSSNEHNH"
     gene            14453..17749
                     /locus_tag="PLF66_10325"
     CDS             14453..17749
                     /locus_tag="PLF66_10325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012476646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HMZ64480.1"
                     /translation="MNTTTDPNSFTSKIIRFSAHNKFLVLVLTFVVLIASYYSMTNIP
                     LDAIPDLSDTQVIVYSRWDRSPDIIEDQVTYPIITGLLGAPKVKVIRGFSDFGYSFVY
                     VIFQDGTDIYWARSRVLEYLSRIQSRLPEGVQTELGPDATGVGWVYQYALVDKSGKNS
                     LTDIRSFQDFKLRYLLNAIPGVSEVAGIGGFKKQYQVTVNPIALQAYNISMESVVQKI
                     RESNQETGARLMELSGAEYMIRGKGYIRSISDIETISLGTDVTGTPILIKNVAKVDLG
                     PDLRRGISDLDGEGDTVGGIIVMRHGENALSVIEKVKKKIMEISPSLPEGLEIVTTYD
                     RSSLILNAIHTLKEKLLEEMIIVSIVILIFLWHFPSAIVPILTIPISIIISFIPMYAL
                     GIHSNIMSLSGIAISIGVLVDGAIVEVENAYKKLEEWESNGRIGDYHAVRLEALLEVG
                     PSVFFSLLVIAVAFLPVFTLVDQEGRLFRPLAYSKNLAMAIAAILALTLDPAFRMLFT
                     RMDPFNFKNKFLSKAATTLFVGKYYKEENHPISKILFKYYEPACTWVLGHPKKVIATS
                     VFLVLLTIPVYLHLGSEFMPPLYEETLLYMPTTLPGISVAEAERLMINMDKKLKAIPE
                     VVRVFGKAGRSDTSTDSAPFSMMETVVLVKPQNEWRQKERFYSKLPKLTHFLFRSFAP
                     DRISKAELIAIMDKELSFPGVSNAWTMPIKTRIDMLSTGLRTPIGIKIQGGSLEEIER
                     LGIEMETVLKNLPGVRSVFAERTAGGYFLDINLKREKLARYGITIANAQDIILTAIGG
                     EPITQTIEGRERYTVNIRYPRALRENVDQLKRILIPTLTDSHIPIGEIADLEYKTGPA
                     MIRDENGFLTGYVYLDTTESDIGGFVDRAKSRVTELVKLPAGYTLLWTGQYENIIRVR
                     ERMKLVIPITLFIIFFLIYMNTKSYMKTMIVMCAVPFSLIGAVGFLFILDYQISIAVW
                     VGMIALMGLDAETGVFMLLYLDLSYEDARRKGLLTNDLELKDAILHGAVKRVRPKIMT
                     VLCAMMGLLPIMWSTATGSDMMKRIAAPMVGGLVTSFALELLVYPAIYYLWKRKELTI
                     ENRTELSPIS"
     gene            17786..18226
                     /locus_tag="PLF66_10330"
     CDS             17786..18226
                     /locus_tag="PLF66_10330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002979351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64481.1"
                     /translation="MNKLKIIFIGICFLSGINAWAHNGKATQVMEEMIKFHDALVKET
                     PGKLNTTKLTELLNKGADREKDAEAFKKAIPLAEKLGKLAGMKEKLDVYAELVEILSS
                     VVGHHDKSNANVFFCPMVKKKWIAKGDTIINPYLKDMRDCGEKQ"
     gene            18329..19600
                     /locus_tag="PLF66_10335"
     CDS             18329..19600
                     /locus_tag="PLF66_10335"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HMZ64482.1"
                     /translation="MTQKEFWKLHSSAHLNFKLNEKIYFILFLIFLASALHILASHFQ
                     NELFIAIAVRLNYIPVFYAGMRGGLIPGLLSGFIVASLHSIIMLHFGHATHGSHLILT
                     EHLVETPFIVLFAALIGFLRDHEQMQRAKQEKIKEIFGKYVSQNIAEQIINNEIVLTG
                     EEKVATILFCDLKGFTTLSEKLSPQDLLNLLNYFFSEMVTIILRNGGFLDKFIGDAIM
                     AVFGIPFNEEKHAEIAVNVAIEMQKKLKELNESKSFGEFQLAMTIGIHTGNIIAGNLG
                     SKERMDYTVIGDTVNLASRIQSLNKSYSSDILISDSTYKSLIGSSNIFAREIDTVKVK
                     GRNKPCILYEILHNKNIHSFNHKIKTLETFSSAILLYKSGDFISSKNLFLTLIDNDNY
                     DLLPAIYIERINTLLKNPPENWSGIYEHTNK"
     gene            19669..19863
                     /locus_tag="PLF66_10340"
     CDS             19669..19863
                     /locus_tag="PLF66_10340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64483.1"
                     /translation="MGTLMIGVMVLMMGGMILMHTAMMNHDSKDHKEGQHETAKKTTE
                     SKAKTSEELETKSSEKEHKH"
     gene            19910..20254
                     /locus_tag="PLF66_10345"
     CDS             19910..20254
                     /locus_tag="PLF66_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002979339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64484.1"
                     /translation="MKNSKITISVLLYIVTSIAIFSQDVPKIKAEHLKMVAKYEEQVK
                     AQGMIAEEHSKMKKEYVKKYFINEKLSNRSTINEMEKHCDKIIEEANQLKKNFQMMAD
                     FHKSLAAELDKK"
     gene            20497..20787
                     /locus_tag="PLF66_10350"
     CDS             20497..20787
                     /locus_tag="PLF66_10350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004788199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64485.1"
                     /translation="MSLKKLLEKGADSKKDTESFKKAIPILEKLGQSKALDEKLNLYA
                     ELVEILSPTVGHHDKSGANLFYCPMVKKKWIAKGDEITNPYDKSMRNCGEKL"
     gene            complement(20986..21777)
                     /locus_tag="PLF66_10355"
     CDS             complement(20986..21777)
                     /locus_tag="PLF66_10355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64486.1"
                     /translation="MDWLDKKNTGVMKFIILVLLINAFSCSIKSTDQNPIPAKKEDLQ
                     LKKSTVIFAGNGKWKPEFNAPGWNFTFYKKEEYEQFFAGEGSCLIKGKYKQINNTIQL
                     FRKKTENCSPFSESEFTTCNIVETPESLYSLFALKCNSNLYYSIEQKRNTGDRVIIGR
                     VLHEIIYPNDYETTDNVFFRQGPGVNFEPINCYYSETETQIERRNVLHKNYRLYVIAK
                     TVDKTQVGKFTNHWYYVKPEQDFYGRKYCSLAEGWVFGEYIRKLK"
     gene            21917..22726
                     /locus_tag="PLF66_10360"
     CDS             21917..22726
                     /locus_tag="PLF66_10360"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HMZ64487.1"
                     /translation="MSDKIEIQMPTFYPFMFEMPIRKTDVTLDIHVSFATILDIVFEG
                     HMRFFKFMGHDVTDVYGRSLIFANAVILYKGELLADDVVRIEVRADNFLEKGCDLFFH
                     LTKDGGKIPVADVKIRMLFFDYTIRKVALVPEEFKAKCLATLKNDPTQQKAIPKTNES
                     PLWNKAHQFVLNLYKSTNQFPKEEMEHLIPRIRKSATSLPLSILEVTNRKEKSDIVKS
                     FGKTRGFIEELRYYLILANDLNYLNASNLIKELDDLNTELRENYKKTISAL"
     gene            22737..23504
                     /locus_tag="PLF66_10365"
     CDS             22737..23504
                     /locus_tag="PLF66_10365"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016548764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HMZ64488.1"
                     /translation="MNSFEGKVAIVTGGTSGLGRSIAVELAKRGAKVAFCGRRDQEGN
                     DTLSIIEKSGGAGFFQKVDITKAKDIEDFTQNVINRFGKLDIAINNAGIIGKASISTK
                     YEEEEFQKVYETNVKGVWLSMKYQIPEMLKTGKGAIVNISSVSGLIGFPFNAAYSASK
                     HAVIGLTKSASLEFSHKGIRINTICPGGIETDMLENIFESTGKPDLAKSNMTNLHSMR
                     RLAKPNEIANAAIWLCSEEASFITGIAMPVDGGWTSQ"
     gene            23679..24206
                     /locus_tag="PLF66_10370"
     CDS             23679..24206
                     /locus_tag="PLF66_10370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64489.1"
                     /translation="MKRNYKLILSFLMILLTVFFVEACRKKNLPAEFELISSRFGEFK
                     MYDQMLDGKIVKRIIHKEDDVYQSENLQLVQLNQIIQLNTSDMFLFTVESLDDNTQCP
                     AKFLVITLNSEGKFKTFPEIGNCNDLPEVDDNGEQVTFTFNSILSYEAVTYRFENGDL
                     KQIENQKSAARIINK"
     gene            24213..25307
                     /locus_tag="PLF66_10375"
     CDS             24213..25307
                     /locus_tag="PLF66_10375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008597215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HMZ64490.1"
                     /translation="MKTLSTSIQGLDTSPIRKAFELAATLENPINLSIGQPHFACPQN
                     IIEALTKAANDGKTAYTLTGGIPELKEALVRKYETVNKIKYATPSRILVTSGISSALL
                     LLFSALVNPGDECLIISPYFLMYPSMLKFHGAKIHLLDEKFTKEDTKKLKFSNLKLII
                     YSNPSNPTGYVMTKEQLEALADLAEVNDAYLISDEIYELFDYDKKFLSVGSFYEKAIT
                     LSGFSKTYSMTGLRLASILASEEITKVLTTLQQYTIVCAPSVTQYAGIEALKTDMSSY
                     IKEYKENRDYVYDSLKDYYELDKSAGAFYFFLKVKGNDEEFVKKAVLEKKLITVPGFI
                     FNNSHSYIRISFANTKENLTRGINALKELA"
     gene            25300..26088
                     /locus_tag="PLF66_10380"
     CDS             25300..26088
                     /locus_tag="PLF66_10380"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002974304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HMZ64491.1"
                     /translation="MLSIFFIYFYGFWIASSIASFYTTLGERILKYYYSPLRKKYSFF
                     EKWKIIFTKPSACENCGTQVKAIYLTPILGYLFTKGKCPSCEIKIASHYPILEFLFGI
                     LFCIFLSLTNNILFSLSVLFLFGHVLVSMITDYKKFSLDYENLPFLLFFGVLSNFLFY
                     NQFPDFIDYAVFTGFVAFYILLWVFYKKGIGLGDVIFAPVYAFLAGHPYWILFLNSSY
                     ILAVLWAFLSRKKGEKLDGKPIPMGFFFGIGLLISFLGKAIMAR"
     gene            complement(26294..26554)
                     /locus_tag="PLF66_10385"
     CDS             complement(26294..26554)
                     /locus_tag="PLF66_10385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008589098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HMZ64492.1"
                     /translation="MKKNEKELLESIEAGEWKSVKNLKEWNLKLKKAASATLIKDARM
                     NIRIHKRDLQLLKVKALEEGMPYQTLVSSILHKYVTGKLKEI"
     gene            complement(26541..26831)
                     /locus_tag="PLF66_10390"
     CDS             complement(26541..26831)
                     /locus_tag="PLF66_10390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011669463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HMZ64493.1"
                     /translation="MVFNWDNGKNDELKQSRDISFEKIVIAIEGNKIVDVLEHTNKER
                     YPNQVLLLVNLDDYIYVVPTVVDMKKEEYFFKTIYPSRKFTNQYIGAKNEKK"
     gene            27041..28171
                     /locus_tag="PLF66_10395"
     CDS             27041..28171
                     /locus_tag="PLF66_10395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64494.1"
                     /translation="MKEYILFLKDKSILSALVVLILFELFLQAGFYKSFLKKNSYASN
                     VNRITDHSLGKQSELDPDILIVGTSLAYEGLSMQALNERLKPLNLKAQSIAIPGAELI
                     VQGLALEKVLNKFKRVKYIIHVNEIEMPWTLGIVFSQATLAMAGELDRKRAIEKFYED
                     EYNISYADIAYILIRLWAYRKDLGEFILNPEKRIKDLGKAKKAFKDNLYSYENNYQES
                     LSLYHFKNVKECLTITKDNTNIPQGSNPYHREAIAKTCKLAEESVLSLEENGSTELYQ
                     RRLKNFYKILRDRNIQVINVFPPVSNYLEDFQYGKRVLFWKDKYKDVLGEKIFDLTEI
                     IPKEDNANYFYDIVHVNQKGMRVFTDRLGESLLAYLKEKELR"
     gene            28171..29580
                     /locus_tag="PLF66_10400"
     CDS             28171..29580
                     /locus_tag="PLF66_10400"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002179700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HMZ64495.1"
                     /translation="MLFNSLHFLIYLPIVLVLSNILKDRNQKIFLLLASFYFYMAWNI
                     NFIWLLLFSIVIDYFAAISISRLEKTDKRRKHYLILSLVTNLGFLSYFKYTNFFLGAI
                     NDLNFINSFRFVEYNIILPVGISFYTFQSMSYTIDVYRGVMEAKKSFIDFALYVSFFP
                     QLVAGPIVRAGTFFRDLEARLIVNLDVIRHSFAQILLGFTKKVVFADNLAIAVDRVFT
                     NYASMNAFEIWTGVFAFMWQIYFDFAGYTDIAIGVARLFGFQFDINFHFPFNVTSISQ
                     HWSRWHLSFSTWIRDYIFIPLGGSKVSRLRTHINVFITFLFAGIWHGAAYHFVVWGIW
                     HSAMLSVEREYAQTRVSKFLNEKGGFVYSLFCRIFTMICLGIGLPLFRAKTLTDAFGM
                     LKTMLFLNSNSITSSFSNYGYFNLVALLFVTGYIFEKYNLKKMLESKRAFTLFILANV
                     FMLLCYGVSEAQSFIYFAF"
     gene            complement(29731..30612)
                     /locus_tag="PLF66_10405"
     CDS             complement(29731..30612)
                     /locus_tag="PLF66_10405"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HMZ64496.1"
                     /translation="MKNINSIYGLKTLIVDDTLIGRKIVHSFVNSWEMRNGQFASGQM
                     AIGALLEAKASGDPYQMAIIDYNMPEMNGIELARNVRKYRELDDLCMILLSSVDYPDS
                     LNEIQKAGFSAYLVKPISGSILLNSLQNAWNLFSTKENRIVTQYFKGESVISSDNQQT
                     EVLNSPSSLKFLIVDDNEVNVILLSAILKKQNILPDEAKDGYEALEKAKKNNYDVIFM
                     DIEMPELDGIECFSQMKKIEAYKNRKFFTIATTAHVLEGSEEKFLNLGFDFYLPKPVN
                     LNRVKDLLGKIDLKKMN"
     gene            complement(30636..32279)
                     /locus_tag="PLF66_10410"
     CDS             complement(30636..32279)
                     /locus_tag="PLF66_10410"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HMZ64497.1"
                     /translation="MKRKKTSLFFFFISIFLGIQFVIEADGFILTTEEEKWVKANPKL
                     RVGIHEYPPLAFKTTDGDDGYDGITIQYLQKIEKKIGIQFEFIYIDSWVEVLNQAREK
                     KIDLVFAVQETDDRKEFLKFTEPYITLTNKIVSRKGRDFPNLKSLIGKKIALVESSAV
                     YKYIQTNYPQIKIIPVKDEVNALAKVASGEVDATVSEISRISYFYERELFSNIFISGD
                     VDFKYEFRFGVIQDIAIFSKILDKSLSSLTKPEKDKILEKWIKIKEKSIFEKEIFWQV
                     TVSLILFIAFLKFLNWYQTVNKFVSLRTKQLREAIEKAEIAIHSRTSFLSNMSHEIRT
                     PLNGVIGFANLLEETKLDTNQRSYVQNLISSANHLMNLINDILDFSKLQAGKMEIQMI
                     DCNLHTIIQEILNVFFNEAKQKKISVRLDFPETIPYQVKTDAYRVRQILFNLISNAVK
                     FTDTGYVNIRVSTLNDYDLNKLKYKFEVEDTGIGIPKERIGDLFKEFTPIDMSTQRKY
                     NGTGLGLVISKSIAELLGGMFIICLAQKEKVQFLFLKYI"
     gene            complement(32718..33617)
                     /locus_tag="PLF66_10415"
     CDS             complement(32718..33617)
                     /locus_tag="PLF66_10415"
                     /inference="COORDINATES: protein motif:HMM:NF016955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HMZ64498.1"
                     /translation="MTNLILTLIGIFLILNSREFLFFFLGVFLVVLAIKRMLDEFSGP
                     AIIDNREYVNSLVYKIRNEIRNYSILNQYAAILASACIHIAKSDGRISEKEISVIRQA
                     IHREFQNNVEETLIAQVVSYTKQHIQEFSKEQIFDSLVDIVNLYFQHLSIAGWEARIE
                     LTTMLFLMIYEVALADGEVTQTEEELFNRLCFQFSLPGAYVQNIKRTARYNVNARANR
                     SSSSYSYSYNENGDSVKFKNALTLFNLNENYTLEELEKAWKQMAILYHPDKFHNAKPE
                     IYELMNKKFIEAKDAYEFLKKRK"
     gene            complement(33626..34105)
                     /locus_tag="PLF66_10420"
     CDS             complement(33626..34105)
                     /locus_tag="PLF66_10420"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010412574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HMZ64499.1"
                     /translation="MAITKDDFKKAMGHWASGVTIVTYSDNGIYSGLTASSFTSLSMD
                     PFLVLFSVAKNSASHDKLIQIREYAINILSAEQETLSNQFAKPDTDRNKIVLENGFST
                     QVTKSPLLKNSLCHLDCKLETVYEGGDHSIFVGKVEYAYTDESKRPLLYFYRKYYSI"
     gene            complement(34159..37032)
                     /locus_tag="PLF66_10425"
     CDS             complement(34159..37032)
                     /locus_tag="PLF66_10425"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HMZ64500.1"
                     /translation="MKSSFLLRLKEVVNQYPENIALSDQNQILSFKKLEMRLINISHK
                     LEEIGVKEGDRIALWMKKSSDYILSLLACLRLGCIFIPIDEKTPLKRVSWILNDAEAS
                     WVLTDNLEFITQNESLSIENLATKLIQISEKEKSKNMKGAYIIYTSGSTGNPKGVLLP
                     QDGILNFLDTQMDVFRVKSSSKVLQYLSIGFDASLSEIGISLLSGAELAIKEDNQIKN
                     LESLVSILANERITHICLPPALLPILPIEEIPESLETIIIGGEVCPVDTVRLWASKFR
                     IVNVYGPTEATVCSSLIVCEDNWSKPLIGIPIPNRTFYILDADLKPVDRGEKGELYLG
                     GVGLATCYINQEKLTNEKFITYNGERIYKTGDLVCYHSDEEIEFLGRMDRQFKWKGNL
                     IEPIEIEKILTHRKEIVFSYVSAKEKKLLAYLVLQKDFILDQNYFLELKKSLSIELPK
                     WMVPDQFILLDQIPLNQNEKMDFNSFSKMIESRPFYLKEISPPKNAIEIKLTSILEEL
                     LSCKPIGVEDDFFLLGGDSLSLLSFVTECSLIGIHFPIDKWILNPTIRELLSILKEPV
                     IERKVEFLKREAELPDDLLDLIQTRKKDSKNWKNILLTGATGYLGSRVLFELAADSEL
                     NLYCIVRNRKNAYNQIEFYLGKQGLEIDLDQIYLIEGDVSQKNFGMNLDLYESLTNKI
                     DTVFHLAAQVNLILPYQKLKDVNVKGTLEVLFFCLRGIRKYLHYASTLSVLVSSNDPR
                     KVFSEDRIDYSSEVYGGYAESKWVSEKILLNAIESGCDFIRIYRFGLITGDSKSGFSK
                     KEDFFTQFLEFILKYRILPKLDDLDLKVDMTPLDYAAYRFISIAKSQSNSRIFHIANL
                     KSLTIYDLLEYLKRLEIPIKQISKNEFLEYILNLDKEESYFYLLIARVFISDDAFNSI
                     YPLDLFQKTDREFDFKNTNIFPTSYPILANEELIARYISFLKN"
     gene            complement(37029..37325)
                     /locus_tag="PLF66_10430"
     CDS             complement(37029..37325)
                     /locus_tag="PLF66_10430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64501.1"
                     /translation="MKLIKSSGDLRHFVEKMQKGINKTKLAGIVSFHEEEGGMYVQFE
                     KLGTSKIHYTVTNDSSGFVAKIKKENVAFTHGIFRRDVEKQLVEMMRRFGAEVD"
     gene            37405..37785
                     /gene="crcB"
                     /locus_tag="PLF66_10435"
     CDS             37405..37785
                     /gene="crcB"
                     /locus_tag="PLF66_10435"
                     /inference="COORDINATES: protein motif:HMM:TIGR00494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HMZ64502.1"
                     /translation="MQYVFIFIGGGLGSVTRFFISELTNRILGNHFPHGTLMVNVIGS
                     FIIGILFGIYESRLSTIPVFRQIVFIGFLGGFTTFSSFSLDTLNLFLQTKFLSAILNV
                     FLNLSLCLIAVSLGYTVLSFLNKG"
     gene            37789..38928
                     /locus_tag="PLF66_10440"
     CDS             37789..38928
                     /locus_tag="PLF66_10440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HMZ64503.1"
                     /translation="MISRTVLILSLVSFFTDISSEMLYPILPLYLKSIGFSIVWIGVL
                     EGIAEAMAGLSKGYFGRVSDKIGKRLPFVQLGYALSAIAKPALVLISHPIWILFSRSV
                     ERLGKGIRTGARDAMLSSETSKENKAKIFGFHRGADTLGACLGPAIGLCYLYLYPEDY
                     KNLFLYAFIPGILAIFFTFFLKERQTLSNHTKYQNSIAFLRESPNLYKKILIGLVLFA
                     IFNSSDVFLLLRIKESGLSDLEMILVYIFYNLIYAILAYPMGYIADRFGIKKTFVFGL
                     FIFSLVYFGMSQKGSIIYYLFLFTGYSLYAASTEGIAKAWIANICPRDTATAIGIYSG
                     LVSIALLFASSIAGFLWYQFGAESVFYFSSLGSLMICIYFFRIEE"
     gene            38971..39198
                     /locus_tag="PLF66_10445"
     CDS             38971..39198
                     /locus_tag="PLF66_10445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64504.1"
                     /translation="MNTAEKILNIFKENNIQANEKLHKRIVMDTIKSWGLDVVEIRNA
                     WHALMGYGLLMQVGDELVLTKQGESVAYKNQ"
     gene            39195..39605
                     /locus_tag="PLF66_10450"
     CDS             39195..39605
                     /locus_tag="PLF66_10450"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF025892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HMZ64505.1"
                     /translation="MKDKEEYKIFFESHDALGKVFGAKFISITEEECIYEYEVSKNHF
                     NPNGTLHGGALFSVMDSCQGAFFHYIVDDIYKFGATGTASIRYLAPITGGKIKIKTWL
                     KEKVRRRFILLSTAYDESGKEVATLEEIWIGILK"
     gene            complement(40063..42027)
                     /locus_tag="PLF66_10455"
     CDS             complement(40063..42027)
                     /locus_tag="PLF66_10455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64506.1"
                     /translation="MNIWSVLKIDPTKEERAIKKAYAAILKTIDREKSPEEFQTLRKA
                     FEEALAYTKQDWEWEDLTEEEDNLEAISIEHKEEIEDLKDNSEYPQEEKYYSKQKNDT
                     YQEEEDEFEQDVFPSLKNKIDYQNTIEANRIVRELINLLKEEGEEKAVIKWNHLENEL
                     KEYTFGFLEEFNLSLRNELELLYSDGMPNDEKFPFRFLEKVFSYFEWNDYEEYKYYSL
                     TIIKRKISARRERIRLEKEILKNKRLAPLLEPIPSDPSNIRTSRLQILNIQKYIEHTQ
                     AKCPELFDYEIDSNIVDYWMNAKKEMSKIWILAFIGLPMLAAGVYKNGSVFLNFTVIP
                     ALFFFWGKSLREIIYSYICFFYRLFLSIDFDGKKNRGLIGLVFAILGTIVFSEVFAIA
                     SLQSSATSFFEILLRFIGVILFTILFAPIFDLIIQLFYHFSNAIFVRIPSIRLELWFA
                     GVFLLFLLHIYLGEIDGSRLQNVALLVLLIVGFDLFLSRDKKEIFFLEIYTTFLLAPT
                     TGYYLYLLFGWKSFSAIGFSVASLLAISNFEDVYSTSKIKISGRNIDLYLVFAAILVS
                     IGIIFYPEIPALIIAFLLFFILQIRVTFLPFWQIYWSVFVTLGLFLSPFVRKYIPIDP
                     KISPLLLQLTLFSISILLGKIKWRKRFYHG"
     gene            complement(42027..43727)
                     /locus_tag="PLF66_10460"
     CDS             complement(42027..43727)
                     /locus_tag="PLF66_10460"
                     /inference="COORDINATES: protein motif:HMM:NF012242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
                     /protein_id="HMZ64507.1"
                     /translation="MIIGIDLGTTNSLVSVFQDGKSVLIPNSLGSFLTPSVVSMTEKD
                     EVLVGLSARERLSTHSNLTASVFKRYMGTNKEIFLGRKKYRAEELSSLVLRSLKRDAE
                     VFLGEKIEKAIITVPAYFSDAQRKATRIAGELAGLSVEGLLNEPTAAALAYGLHELAD
                     ESKFLVFDLGGGTFDVSILDLFEGVMEVRASTGDNFLGGEDFRNCIVDRFVERVVRKE
                     KLPEEDQKLIGIIRNQAEIAKRALTESNRVEMKVNWKDTTYTMPITSDEFSEWTEPLL
                     ARLRKPVERALRDSKIKASDLNQIVLAGGATRMPSIKKLVAKMFGILPASSLNPDEIV
                     ALGAGVQAGLKMKNSALKEIVLTDVCPYTLGIEVSERVGNDYKSGFYMPIIERNTIVP
                     VSRSEVVNCVEDNQKVMRVRIYQGEARLVKDNIFLGEVAVRFPPLPASEASADVRFTY
                     DINGILEVEVHVHASGEKYRSVIEENPGVLTPKEIEEKLKSLSNLKIHPREKEENRAL
                     LSRAERLYEESLGNIRRYIGEEMTLFISALDSQDEEKIGKAKERIVYVLNDLEGNNLF
                     "
BASE COUNT        14198 a         7758 c         7203 g        14756 t
ORIGIN      
        1 tgatttttaa aaccaagtct gaggaagtaa ttcctgctgg tttgtttact agataaaaac
       61 cagatttaat tctctgtatc ctcttctttt gcttcagatt cttcttggta gtttcgaatt
      121 ggtggaagat tatctagaaa ttgattcatc tctagagatt ttatataatc ctcatcccat
      181 aaaaaaagga ttttcggcgt taatcgcaac ttgagttgct ctgcaagaat atgttgaaag
      241 taacctcttg ctgatctaag tgcattgagt aaggtttttt tgcctttatc atctgtataa
      301 gaggtcaccc agattttgac gttagtcatg tcatttgaaa gttctgctct atgaacagat
      361 acaataccaa ttcttggatc ctttaatttg ccagaaacaa ttaaatttgc aacagatttg
      421 ataatctctg attctatttt cttttttctg gtttcgttca tattaaaata aagtcagttg
      481 caagattaaa gttttcttgc aaccgaattg atttcatagg attcaatttc atccccttcg
      541 atgaaatcat taaatccatc aacgagtaga ccacattcaa atcctgttag aacttccgta
      601 acatcatctt tcatacgttt gagcgacttg agttttcctt caaagataac tacattgtct
      661 ctccaaatac gaatcaaatt attcttatga atcttaccag atgtaaccat acaaccagca
      721 atatttccaa ctttagagat tttgaatata tttctaactt ccaacttacc attgctcttc
      781 tctacttgtt ctggttcaag aagaccttcc atggcagctt tgatttcatt tacaacgtca
      841 tagatgatgc tgtaatattt aatttgcacg ccttctttct cagcgagtgc caaggttctt
      901 gggttcgctc taacatggaa acctacaatg agagcattcg aagcagatgc tagcataacg
      961 tctgtatcaa caatagctcc agttccagca tgaattacat tgagttttac atcccctgta
     1021 gaaagttttt ccaaggattc cttaattgct tcagcagaac cacgaacgtc agttttgata
     1081 atgatcttga gttcttttag ttctccctga gatatgattt cattcatatt gtcaagggtt
     1141 actttagtaa cagtagcagc ttgaccaatt cgttggtatt cttttctatg ttgagaaata
     1201 tttctagctt ccttctcgtc gcggataacg tcaaacggat ctcctgcatc cggcactgca
     1261 tctagaccag tcactaaaac aggaaaagat ggttcagcca tttgcacatg gtgtccatgg
     1321 tcgttgtaca tggctctcac tctaccagag tgaactcccg ctaagaaggc atcaccaata
     1381 cgtagagttc cattttgtat aagaacggtt gcaaccgctc ccctacctgg atctagtttt
     1441 gcttcaatga ttgttccttt cgcaactcga tttggatttg ctttaagatc taacatttct
     1501 gcctgaatca aaatcatttc taagagttta tcgattccta tattttctcg agcagaaatt
     1561 ttacatacga ttgtcttacc accccactct tcagggagta attctaaatt tgtaatgtct
     1621 tgtagaactc tctccacatt ggcagatggc aagtcaattt tgttaattgc tacaatgata
     1681 ggaacttcag ccgcttttgc atggttaact gcttcaatcg tttgtggttt aactccatcg
     1741 tctgcggcaa caactaatac tacgatgtct gtaatagaag caccgcgagc acgcatagat
     1801 gtaaatgcct catgaccggg tgtatctaag aaagtaatca cacctcttgg agtatgcact
     1861 tgatatgctc caatgtgctg tgtaatacca cccgattctg tatcgataac agcactcttt
     1921 cgaattgtat cgagcaattt tgttttacca tggtccacgt gacccatgat agtcacaaca
     1981 ggtggtcggt ttatataatc gtccggagaa tctttttctt cttgaatgac agtttcttca
     2041 tagagagaaa caattttaac tgcacaacca tattcatctg ccaaaaggat agcagtatca
     2101 ctatcaatga cgttattgat tgtaaccatc atacccattt tcataagctt agcgatcaca
     2161 tcatttggct ttagatttaa tttctttgca agctctccta cttgaacgtt ttccataatg
     2221 gaaatttctt ttggaacggt agttccagag actggcacac cagatgtttt tttatacatc
     2281 tgtttgaaaa atttactatt ttcctgtgct ctttcggcat tcttatcttt gtttttatca
     2341 aaagtgcctt ggttgtttct ctttcgattt acagttatac caggagtagt tgtttctttt
     2401 ggtatttcaa ttggattgaa tgagccaccg ccttgtcctt ggttagatgg tcgtctttga
     2461 taatttcctt ggccttggtt tccaccgcct tggtttggac gcggatgttt ttgatgaaag
     2521 tttctatttc caccaccttg cccttgataa tttccttgag ggcgatttgg tcgattgcca
     2581 cttccctggg aggaattatc tcgattcgac tgttgaccac caactggctt ttgtggtctg
     2641 gagactatcg gatttcgatc tgccgagcga actggataag caggatgtgg tctggaagat
     2701 tgttgacttg tattagactg ctgctgattt atattttgct ttcttgtttc ctgatgaaca
     2761 ttcgtttctt tcgattgctg ctgtgtttga gtttcagaaa cgtctttttc tttctctttt
     2821 tctttttgtt gaaatgtctc cgaaacattt cgattcacga tgattttaga gtcatccacc
     2881 gttttcataa aaggtttggg agctgcttgt tgcctttctg tttcttcctt taccaataga
     2941 tctaaatctt tctttggctt cggtggattg acatttgttg caggagtttc tgcaatagga
     3001 gattttctct taatcacaat tttcttgtga gaagagtctt gtccttgcag cagtttatct
     3061 ttaattgatt tattgtcttt tttttcttcc ataataccta taatactcac aaaaaactaa
     3121 gctacgttgc ctagccctcc tcttcaacaa attccactga ctctgataat agacgtaaaa
     3181 tttgctcgga agttgtcggt ccgactcctg gaatcttagc caaatcatct ggttgcattt
     3241 ctacaagagt ttcaatatct cttactccac cactttcgag aagagtgatc acacgaggag
     3301 ttaatcctgg tatttcactg agaggtgttc ctcttgttga atcttctgtt gattcagttg
     3361 ctttagaatg atttgctttg tgattgttaa ataatcgatc gagtctttct cttgcctctg
     3421 gagacgtcat gtcttcattg tattgagaaa ctgactttat atcgatctta aattggcaga
     3481 gctgaactgc taatttaacg ttagaaccgc tagccccaat tgcgagagag agagaatcat
     3541 caggcacaac aacaagcgcc tctctatttt catggtccac tcttacttcg actggctttg
     3601 caggtgaaat tgcatttgta ataaattcag atgggtcatc tgagtattgg atgatatcaa
     3661 ttctttcatt tccaagttct cgaacgatag attggattcg aactcccttc ataccaacac
     3721 aagctcccac tggatcaata tcgcttctag ttccacgaac tactattttg gttcgaacag
     3781 atgggagacg agcgatatct acaatttgaa ccaaaccatc atagatttcc ggaatttcca
     3841 tttcaaagag tttctttacg aaatcaccag aagcgcgaga aagcgtgatc acaagacctg
     3901 tgtctctaaa tttatctttc ttaagttcta ctttagatat aattgctttg agtcgatccc
     3961 cacttttgta tttttcacca ggattttgct cttttttagg cataacccct tctacttttc
     4021 caagatcaat actcatcgta tctttgttga tccatcgttg aaaatagccg tgtgtaagtt
     4081 cgccttcttt gactttgtat tcgttgtaga gtagttctct ttccatctct ttcaatttct
     4141 gaaagaccat ctgtcgtgcc tggttggaaa cgatacgaga aagttcgact ggtttttctt
     4201 tgaatgcgat tgttgagcca atagtcgcat ctggatcaac ttgctttgcc ttatcaagac
     4261 taatctcaag aggagagtca ctcggagcct ctacaactgt ttttgaaatg acgatgacta
     4321 cttcattctt atcgctaaat tctacattga tattgttgac tttggtagaa tctagaccaa
     4381 gcttcttttt gtatgcacct aagagcgagt ctttgatgat acttaaaact gagtctttag
     4441 acagaccttt gtctgcgcaa aactgttgaa ttgcttcaaa tagatttgat tcgttgttta
     4501 cttgtttcgt tgccatatta aaacttaatg tataaattac cttttaaaat atcattttta
     4561 tccaaaacca ctctatttcc caaagacttt ttactcttct ctgtcaaagg ttcaagagtt
     4621 acctgcgttc catcaaatcc catgatttga aaaacattat tcactttctt acctttttcc
     4681 aaataaaaaa tcttcacagg aacattatga aaacgcccca gatcctctgg tagcttcagt
     4741 tctctttctg cccctgctga tgaaacctga attgtgtaat tttcttgccc atgtgtagct
     4801 tcaagaaaat ccgtaagtcg tcttgaaact ttttcgcagt ccgttataga aaccgaaccc
     4861 cttgggtctt ggagattatc cagattaatc tcaatcagaa aatgaccatt ggcaaattgt
     4921 aatataaggg aatacaattg aattggatgt tctataattg tagctaatgc ttccctaatc
     4981 tcagattctt gtaatgccaa taataaacct tttctccaga caacttttaa cctattctaa
     5041 aacaaatcgg gttaaaattt cacaataaaa agcaaggcta gggtgtaaag taaaaaataa
     5101 ttttgttgat tcaaatgtat tccgatcaat gcaaattctc tttcaaatca tacatttggt
     5161 agaaaagagt tattacctca gaacttagaa tcagttcgta gtccatttga tttacattca
     5221 tttctgactt gcattacaag ttcctttaat taaaagcaac tttcactata tctagactat
     5281 acgaaccact atagcaacca gtataccaag tagctagaga gtgataggct ataaaaaata
     5341 gacaaagagg aatcctgact agattcttgc aagctccgaa tcaatgtctt ctattttgta
     5401 ggtcttttct ttttcttcca ttgacagatt tttcttttta gatataccta atttctgatc
     5461 taatcccaaa tataaggaaa ggccatgtta aaaactctat tctttctatt gattctaact
     5521 cattccattt atacagaaga aacgatccaa gtagaaggtt ttacacaaga agcaattcta
     5581 tcagaagaag agatttcttt tgcaggagaa aaaacagtct ttataaaggt gactgcaaat
     5641 ggctacttat catcgggcac agaagattct tacttagaag taataggaaa aaaaatttct
     5701 gaaaaaaaac aaagagtttt cttacttcta ccaagtgggt atgagcctaa aatttgggtg
     5761 aaggagtcag aatccatatt tccaaagcat gaaatcaaat taataaggcg atatctgcac
     5821 gatttatgtt ttgatagaag acaactaata ggaagtgaag cggattatag ctattattct
     5881 aaactgactg aagaatgcta tgtggataaa actagagatt atttaaaagg aaatcgcttt
     5941 cagtatgccg tatatcgcta ctttggaagg atccctgagt ggacagatcc attcgatttt
     6001 atactaggat tttttcttta tattttactc tttctatctt ctctgcctca cctactttta
     6061 tttttattta tctattcttt tttcaaagag tattttagaa aggaagaata caaaaagaaa
     6121 ctcatgacag tcctttttcc atttatctac ggatttttat gggtctttgg atcaatttat
     6181 atcggggaaa catttcgctt taatttttgg ggaatgggtg tttggaaatt ttatttttat
     6241 atctcaggac ttttttttgg acttggattc ttaggaatca aatctgttgt aaatcgagtt
     6301 tttcatcccg aagaagatgt tttaccagat tgggcaattg gtcttattct attcttttct
     6361 cccctactca tctttgctgc aggagctggt ggaggcaaac aacgttcttc ctcttcaaac
     6421 caaaatacga attcttctaa ttcacagtct acttcgggtg gaggtggaac attcggcggt
     6481 ggcggtgcta gtggtggtta ttcttgaatg atatttttca atctctttaa aatcctctgg
     6541 agttaaataa aaatttattt gtatactgtt cattttgttt gtttcccaac ctgatgtagt
     6601 tcgatactat ttttatcatc aatattagct acaattaatg tatgcctatc acctcaaaac
     6661 ccattgcagt tgcggcagta gataattgac tatcatgggt taagaattga atttgccatt
     6721 ttggattttc ttcctttaaa agtatggcag atgccaaatg aattgcatct aacgttccta
     6781 ttaccgtagg aaatggctct gctgactttt gtttcactgc ttgatttaac tcaatcacat
     6841 aaattgtttt ataaaattct aaaaatgttt tatgaagatc agcgtattcg ctatcgtcta
     6901 tttctttttc taaacgcaga cgatttagaa cacgactcac ttcaatataa agaagctcac
     6961 ttgtatagca agcctgccaa ttttgaaatt ccgaataaac cttcggactg ttaagtaacc
     7021 agcgaaggaa agcagatgta tctaaataga ttatcaaaac cgaccacctc ttctatcctg
     7081 caagacatac tctacagggt ctaattttac cttgccctta aatgtatttt ttactttttt
     7141 tgttttacta actggagcct gaactactag ccgagatttt ttggaatatg gaatcagctc
     7201 tgcaactgga tggtccctat ccatcactac ataacgaatc ccattcacta cctctttcaa
     7261 ggtcgcactc aaatgggttt ttaaatttga aattgatatt gtttccataa tttctattgt
     7321 agtcatcaca agactacgtc aagactaaat tccgaagttt ttatcgaaat gatttcaact
     7381 taagcccatt ttcttttcaa agataactac tagaacctat caacttctta gttatgttaa
     7441 ggttacaggg agcctgtcgg caacttaggc gaacgcccaa actcgtgcct ttctttagtt
     7501 taatgtttat tgtaagcttt ttaagttctc agatttttcc aagcttcgta gttactttca
     7561 aagtcaatat tcaaatctct agcttctaaa tttttgtaaa atataaatga gacaagttat
     7621 actctatttg attctaaatt tttttgattt ataaaatatt ctttccttct tctagaaaat
     7681 ataaacaatg aattcgctat gaaaataatc attttacttt tattactcct ttcgttctct
     7741 ttctgctcat ctaaaacaga aaaaccaatt gcagaaagaa cgttagatgg ctgggcaggt
     7801 catccgaatg atccaaataa aaaaccattt gagtattatt acatgacaag tcctggaaag
     7861 gcatcgccaa aatctattca gagaaaaagt ggaccgatgg cgcaatcaac ttgtaccgat
     7921 aatgcattgc taggcatgtc tggcgattta atcgggaaaa tgattggaga tattgctgaa
     7981 gctggatcgc cagcaagtgg ttttctatta cccccaatac aactcaaaaa acaattaaca
     8041 aaatccaggg aaaattttga attcattgac ttatctattc ttgttggatt ccctacagaa
     8101 gaagaatcaa gtaatggtaa taaaaaagaa agaagaccaa taccaattga atctagggga
     8161 ttcaaagaat gcaaaccgac agcaactccg atacctgatt tccctttctc tgaatggaga
     8221 gaatgtgcat gtctagttta tgctcacgtt aaaggcggaa aggaaaccat tgtaaaatac
     8281 tataaagaaa catatttaaa ataaactaaa ctgctaaagc ggattttttt ctaaactctt
     8341 cttctctcac catatcggag agcttaccgc ctgtcgtttt catccagact tctgttcttt
     8401 ctgaaattaa ataagattca aacccaggaa gtttaacgtt tttaaaattc ttttttatcc
     8461 atttaaaaag ttcagaagac atttttaaaa atgcatccgg ctttgaaaca aggagatttt
     8521 gggaattata ataactgtgg atataataaa ttcttccact atgaataata tttccactaa
     8581 taatgttctt aggaattaaa aattctataa cgggagaaga gattgcatca atcgaataat
     8641 aattttgtct ttcaatgaat tgattgttaa tttgctctat gaaatcgcct gacgtaacat
     8701 atttgataga acttatttta ccaagtattt tagtttgcaa taaagaatcc aatttttgaa
     8761 gattagaatt tgtcgaataa ttattgtaaa ccgacaaatc tttctcttgt agatatttat
     8821 ctatctcaga aatgtcctcc ggcattaaat aaaaatttat ttgtttactt ttcatgaatt
     8881 ctaatccttc ttattataac tatgaaacat aggtttaatt ccataattct gagtaacttc
     8941 ttctattgta tcccgctctc tcttaaccgg tcttccatcc tgattttgtt ttccaacttg
     9001 atgtatttct acgatctgat tattttcttc ttcgattgca actacatcca taaattttcc
     9061 cttaccatta aacaaacgaa gaaacttttc aaaaaccgct aagaatcctc tttctttaat
     9121 atcggttgcg acttctttta cttttgcttg atgctcgatt ccaccttttc ttccataagg
     9181 gttagggata ttcttgtcat tgctcataga tttgtctaga ataaaataca atataaatga
     9241 gacaagatag actctatttg attctaaaaa ttcttgattt ataaaataaa tctttccgtc
     9301 ttctaggaaa taagaaaaat gaattcgcta tgaaaataat cattttacta ttattactcc
     9361 tttcattctc tttctgctca tctaaaacag aaaaaccaat tccagaaaga acgatagatg
     9421 gctgggcagg tcatccagat gatccaaata aaaaaccatt tgagtattat tatatgaaac
     9481 atgctagtac ggctagtcaa aagtctatta agagtaaaaa tggagatttg attcgatcaa
     9541 cctgtataga ggcaaataaa agtcaggcaa aaaccaactt aattcctata ttgattggaa
     9601 atactatttt ctcgcaaagt tctcccggcg gtcagcttcc aattgactcg tcactgaagt
     9661 atttacactg taataatgat aacttttatt ttaatgttag aatagatccc ttcaacctcc
     9721 gaaatataat cgctcttgaa atatgggaat tggaaaaatt actcataaaa gaatataaaa
     9781 aaattatcaa agttgtagag gtaaaagact gtaagccgct cgcagttcct gatcccgaaa
     9841 tcccactcag caaatggaaa gattgcgaat gtcttgtcta cgctcaagtt cctggtggaa
     9901 gagatgcatt tatcgtaaga gcccaaaaga ttgatacgga ttgagatttc ttatattcaa
     9961 aatgaatcct gtaacaaaaa tctcttatgt tattcactgc aatgccggta tgtgaaaaag
    10021 ccagaaggga gaattctgcg tattaatgcg atagtgcccg cagcttcggg tttcttttct
    10081 tttttgcata cttttttctt ttctttacaa aaagaaaaga aaaaagtatg atccccccgc
    10141 tgggaagcgg aaataagaaa aatctttttt agtatttgta gaagtctgcg cgactagacc
    10201 tagtcgcatt aaaataaagt ttgaacgact tttcaagtcc ataaaaagaa aaaaaccgag
    10261 ctttttaggg ctcggcaaga aacatcacgg agatttatta cgagataaac ccagattttc
    10321 tatcttagaa gttgcaaaac attttgtggt ttttggttag cctgagctaa catcgctgta
    10381 ccagcttgag ttagaatttg atatcttgtt aggcttgtca tttgttcagc catatcagtg
    10441 tctctaattc tagattcagc agcttgaatg ttttcataac cattcattaa gcctttagca
    10501 gcgtgctcta atctgttttg gtaagcaccg aggtcagctc tttgtttaga aatcatttta
    10561 agagcgtcat cagctagacc gataacagag tttgctttgc taagtgtgga aagagagata
    10621 aatgtcaata cagttgggtt tcttaagcct aaagctgcag ttgtcattgt ttcaatgtaa
    10681 actctttctc tttggtgcat gtttgctccg atatggaacc acatactagc agttgggttt
    10741 aatctagaaa acgcaccagt cataagcttc attttgttga attcagcttg agatgcaatt
    10801 ctatcgattt catcaatcaa ttgagaaacc tcaacttgga tttgctgtct gtcttcttcg
    10861 gtataaatac cgttagccgc ttggacagct agtactctaa ttctttgaac gatttcttga
    10921 gtttcttgta accagccctc agttgtttga atgagggaaa tgccatcttc agcgttcatt
    10981 tctgcacgtc tgagtccgtt cacttggctt ctcatttttt cagatactgc cagtccagaa
    11041 gcatcgtcac cagctctgtt gattctcatg cctgaagaaa gtttttctac atctttattc
    11101 aaagcttcgt tgttgaattt taatactctg tgcgagttaa tcgcagatac gttatggtta
    11161 ataatcattc ggtttcctcc atgaatccga ctttcgtttg aaagtaatga tattttgaag
    11221 aatcatcctt gactcttcat gtaatagatc gaccagcctc aaaaattcaa tgagacactt
    11281 tttttataaa aaaattattt tttttaaggg gaaagaaaaa ggagactcgg agctgacggg
    11341 actcgaaccc gcgacatcct gcgtgacagg caggcactct aaccaactga gctacaactc
    11401 ctcgaggtat attccaaatt atttaacatc tttcataggt caaacctttt tcttaaaaaa
    11461 gttcttgctt ttttatttat tggtgtctaa aagagtgtta tgaagaaaat atttctttta
    11521 ttactagctc tctctcttgt attcagtgca gattgtagag aacttatgac taaaaagaat
    11581 atttgtcatt tatctgttca gaaagcaaag acatctaaag cggactgtca tggaaacaaa
    11641 gaaaaaacca aaaacagctg taaatgtcct gagactaaag cgacacaatt acaagatact
    11701 agcttaaaag agtctagaaa agttgttaaa ttagaactcc tctttacatc acctatcaat
    11761 ttagttcatc ctatctccat tttaacgaaa accgattctg cctatcttct gaacttatct
    11821 tatttctaca cattttttca tcctacagaa accacacatc tactcatttg agatttcccc
    11881 ctacaatctg ttgatttaga aattcattcc cgaaattttc tatcgggaat ccacaagaat
    11941 tttgttttgt aaaaaaggga ctaccaccga ggacaccgat aacggaacga tttaagttga
    12001 tccgatagct ttctaaaact tttcttgttt tgatctaaat cattttcttt caataaaaaa
    12061 gtttaatacc caaggaggga acaatgaaac gaactttata cattatacta atctttttac
    12121 taacgttagg tttatactca gaagacattg aaattcaact agaaaatctg atttcactcc
    12181 atccagaaat caaatctcta gaagcagagc taaaatctaa aaaagcaaac gctggacacg
    12241 aaacgactta tccagatcca aagataggaa tcgcatttcg aaattatccg gcgagatcat
    12301 actctttgaa tgataaagcc tatgatactc ccagtatgac tggaattgaa tatagcattt
    12361 cacaagaaat tccatttccg ggaagactca ctctacaagg aaaggtaagc aaattaggtg
    12421 aattagaatt cttgcatcta tctaaatccc gtaaaaacca atttttacta gaatacctta
    12481 cacaactggt cagactaaaa agtgcagaga agaaaatagt cttaaatgaa ttaatccgta
    12541 aaacacttga aagtcaaaaa accatttcaa cctctacact tgcagtagga gggtcaaatc
    12601 tttctaaatc cttaaaacta caagtagaag caagtctttc ccaggaaaga gaaattgaat
    12661 ataccaaaga caaaaaaaat gcgatagccg ctcttgaata cttcaaggta gatacgtcta
    12721 gtaaggactt acttataaaa ttagatccaa gtaaatattt aaaggaaaaa gcaattcaaa
    12781 tcaaatcaga aaaaaatatc ttaaaagaaa acttaataga aaatccaaac tacaaacttt
    12841 cctcggttgc aatcgaaaga gcaaagaatg aaagtaaact ttcttctctt ttacatgcac
    12901 ctgatacaga ggttttcttt gcttatatga aaagaagaaa tcaaataatc accttggata
    12961 atggacctct caattatcaa attatggata atacagaata tcggggagac ttattttcct
    13021 tcggagtaaa tgtccgcgtc cccgtttggt cttttttttc cagccacgat ttaaaacaaa
    13081 gatcagaaga gaatgttcaa tcaaaggcat ttgaactaga gagaaacaaa ctttttctgg
    13141 aatctaattt tgaaaaacta cttgtgactt tagattcaat cgatctccaa attgatcttt
    13201 atgaaaaaag gattatccca gagttaaata aaagtttatc agcactatct tctcaatatg
    13261 gaactggaaa agtagattta atcgaagttc taaattctaa aatagaatta caaagagcat
    13321 ttataacaga agaagagcta caggagaaaa agaaaatcac taccctactc ctattagaac
    13381 taacagatca tttattaaaa cccaaagaag gaaactaata tgaaaaccct attactctac
    13441 attgttctct cttttacttt gattggattt ttcgaaagtt gtaaaaaaga aaaagaaatt
    13501 tggtattgcc caatgcatcc aacgtataca tcggacagag ccggtcaatg ccctatttgc
    13561 aatatgactt tagtgaaaaa agaaattctt cccgcgaagt ctacgtctca tagtgaacac
    13621 gatcacgaga atatgcctgc ttcaactggt gataaaaaag aagagaatac tatccattta
    13681 ccagaagaga aacaaagact catcggaatt aaaacagctc gcgtagaatt aattcctctt
    13741 agtaaaaaaa tcgtcgccta ctctagagta gcctatgatc cggagttgta cactgccatt
    13801 ctagaataca gagaagcaaa aaaagctagt caaattctaa ccgatgattc tttacctaat
    13861 tcgagtagct tagcaaatgc aagcattgtg cgtttaaaac aattgggact ttctgactct
    13921 caaatttctg aatggggaaa ttctaatcgc aatccagatg aattaattct aggaagcaaa
    13981 aatggaagag cgtatattta ttcttccatt tatgaaacag atattggcta tgtgaagaaa
    14041 ggtttaaaag tatcactcaa aatagattcc tttccagata aaatttttac cggcacaatc
    14101 caaagtatcg actccatatt agatgaacaa aatcgtactc ttcgttttcg ctcatatgtt
    14161 tctgataggg ataatatcct gaaaccgcag atgtttggca atatagaaat ttcgattccc
    14221 ttaaagaaag ctctatctat tccaaaatct gcacttttaa ataccggcaa acacaaatta
    14281 gcctacaaac gagtttccat tgaagaattt actcctgtga tggttaagac tggaatggag
    14341 acagatgatt actatgaggt tctagaaggt ttagaagaag gaaatgaaat cgtagtagag
    14401 tccaattttc tcctagactc agaaagtaaa attaaactag gaggatcttc caatgaacac
    14461 aaccactgat cccaattcgt ttacctctaa aatcattcga ttttcagcgc ataataagtt
    14521 tttggtgcta gttttgactt ttgttgtatt gattgcttct tattattcaa tgaccaatat
    14581 tccacttgat gcgattcctg atttatcgga cacacaagtg attgtttatt ctcgctggga
    14641 tcgaagtcct gatatcattg aagatcaagt aacttatcca attatcactg gattactcgg
    14701 agcacctaaa gtaaaagtga ttcgcggttt ttctgacttc ggctattcgt tcgtatacgt
    14761 tattttccaa gatgggacag atatctattg ggcgaggtcg cgcgttttag agtatctatc
    14821 ccgtattcaa tctcgccttc cggaaggagt gcaaacagag ttaggtcctg atgcaacagg
    14881 tgtaggttgg gtttaccaat atgcactcgt tgacaagagc ggcaaaaact cattaaccga
    14941 cattcgctct tttcaagact tcaaacttcg atatttatta aacgcaattc caggtgtttc
    15001 agaggtggca ggaatcggag gatttaaaaa acagtatcaa gtaacagtaa atccaattgc
    15061 tcttcaagca tacaatatct ctatggagtc tgtcgttcaa aagatcaggg aatccaatca
    15121 agaaacggga gcaaggctca tggagctaag tggtgcagaa tatatgattc gcgggaaagg
    15181 atacattcgt agcatttcag atattgaaac cattagccta ggcaccgatg tcacgggaac
    15241 tcctatctta attaaaaatg ttgcgaaagt cgacctaggt cctgatttaa gaaggggaat
    15301 ttctgattta gatggagagg gtgatacggt tggaggaatt atcgtcatgc gacatggaga
    15361 aaacgcattg tcggtaattg agaaagtaaa aaagaagata atggaaatta gcccttcttt
    15421 gccagaaggt ttggagattg taacgactta tgaccgttct tctctaattt taaatgcaat
    15481 tcatacttta aaagaaaaac ttttagaaga aatgataatt gtttcgattg tgattttaat
    15541 tttcttatgg catttcccat ctgcgattgt ccctattctt accataccga tttcaattat
    15601 tatttcgttt attccaatgt atgcacttgg aattcattcc aatatcatgt ctctctccgg
    15661 aatagcaatt tccattggag ttcttgtgga tggagctata gtagaagtag aaaacgctta
    15721 caagaaatta gaagaatggg agtctaacgg tcgaatcggc gactatcatg cggttcgtct
    15781 cgaagcactt ttagaagttg gaccttctgt atttttttct cttcttgtaa tcgccgttgc
    15841 cttcttacct gtattcactt tggtcgatca agagggaaga ctctttcgtc ctctcgccta
    15901 ctctaaaaat ctcgccatgg caatagcggc tattcttgct ttgacgcttg atcctgcctt
    15961 tcgaatgtta tttactcgaa tggatccatt taactttaag aataagtttt taagcaaagc
    16021 ggctacaacc ttgtttgtcg gtaaatacta taaggaagaa aatcatccca tcagtaaaat
    16081 tctttttaaa tactatgaac ctgcatgtac ttgggtatta ggacatccca aaaaggtaat
    16141 agctacctcg gtatttttag ttttacttac cattcctgtc tatctacatc ttggctctga
    16201 gttcatgcct cctctctacg aagagacctt gctttatatg ccaacgactc ttcccggaat
    16261 ttctgttgca gaagcagagc ggctaatgat taacatggac aaaaaactaa aagcaattcc
    16321 tgaagttgtt cgagttttcg ggaaagctgg aagatcggat acatctactg acagtgctcc
    16381 tttttccatg atggaaacag ttgtattagt caaaccacaa aatgaatgga gacaaaagga
    16441 aagattttat tctaagttgc ctaagcttac tcatttccta tttcgaagtt ttgcaccaga
    16501 tagaatttca aaagcggaat taattgctat catggacaaa gaactttctt ttccgggtgt
    16561 gtctaacgca tggactatgc caattaaaac aaggattgat atgctctcga ctggtcttag
    16621 aacaccaatc ggaattaaaa tacaaggagg gtctttagaa gaaatcgaaa gacttggaat
    16681 tgaaatggaa actgtattaa aaaatctacc aggtgtaaga tcagtttttg ccgaaagaac
    16741 agctggcggg tattttttag acatcaacct aaaaagagaa aagcttgcac gctatggaat
    16801 tacgattgca aatgcacaag acattatatt aacagcaatc ggcggggaac caatcacaca
    16861 gactatagaa ggcagagaaa gatatacggt taatattcgt tatccgagag cacttagaga
    16921 aaacgttgac caactcaagc gaattctaat tccgactctc acagattctc atattccaat
    16981 tggagaaatt gcagacttag aatacaaaac tggacctgcg atgattcgag atgagaatgg
    17041 atttcttaca ggttatgttt atttggacac aacggagtct gacattggtg ggtttgttga
    17101 tcgcgccaaa tcaagagtaa ccgaacttgt aaaacttccc gcaggttaca ccctactctg
    17161 gacaggacaa tacgaaaaca tcatccgtgt aagagaacga atgaagttag tgattcctat
    17221 cactcttttt atcatcttct ttttaatcta catgaataca aaatcataca tgaagacaat
    17281 gattgttatg tgcgctgtgc ccttttctct catcggtgct gtgggatttt tatttatctt
    17341 agattaccaa atttcgattg cagtttgggt gggaatgatt gccctcatgg gactcgatgc
    17401 agagacagga gtatttatgt tactctattt agatttgtcg tatgaagacg ctaggcgaaa
    17461 gggactctta acaaatgact tagagcttaa agacgcaatt ctacatggag cagtcaaacg
    17521 ggttcgtcct aagatcatga ctgtcctctg tgcgatgatg gggttactcc cgattatgtg
    17581 gtcaacagct actggctctg acatgatgaa acgaattgcc gcacccatgg ttggtgggct
    17641 tgtgactagt tttgctttag aattattagt ctatccagcg atttattatc tttggaagcg
    17701 aaaagaatta acaatcgaaa atcgaactga gttaagtcca atttcttaat tcagtttttt
    17761 gaattcattt taaaggagaa taacaatgaa taaacttaaa ataattttta taggaatctg
    17821 tttcctatct ggaattaacg cttgggcaca caatggaaag gcaacacaag taatggaaga
    17881 gatgattaaa tttcatgatg cattagtgaa agaaacacct ggtaaattaa acactactaa
    17941 gcttaccgaa cttttgaaca agggtgctga tcgggagaaa gatgcagaag cttttaaaaa
    18001 agcaattccg ctcgcagaga aattgggaaa gttagcaggc atgaaagaaa aattagatgt
    18061 ttatgctgaa ttagtcgaga tactatcttc cgttgttggt catcacgata aatcaaacgc
    18121 aaatgttttc ttttgtccta tggtaaaaaa gaaatggatc gcaaagggag atactatcat
    18181 taatccatat ctgaaagaca tgcgagattg tggcgaaaaa caataatgat aaaagccaga
    18241 atcaggaaaa gaaattaaat agaaaatgaa aacaacagga actgcgtagt gaatatgatt
    18301 acacagttct cgttggttag aggaaattat gacacaaaaa gaattttgga aattacatag
    18361 tagtgcacat ttgaatttta aattaaatga aaaaatttac tttattttat ttttaatttt
    18421 tttagctagc gctttgcata tccttgcatc acattttcag aatgaattat ttattgcgat
    18481 agcggtaagg cttaattata ttccggtttt ttatgcgggg atgcgaggcg gattgattcc
    18541 tggtttactg agcggattca tcgttgcgtc cctacattca ataatcatgt tacactttgg
    18601 tcatgcgaca cacggtagcc acttaatttt aacagaacat ttagttgaaa ctcccttcat
    18661 tgtattattt gcagccctta ttggattttt acgcgaccat gaacaaatgc agagagcaaa
    18721 gcaggaaaaa attaaagaaa tttttggtaa gtatgtatca caaaatattg ctgaacaaat
    18781 tattaataat gaaatagtat taacaggaga agaaaaagtt gctacaatcc tcttttgtga
    18841 tttaaaagga tttacaacgt tatccgaaaa gctatctcct caggatttat taaatttatt
    18901 aaactatttt ttttccgaga tggtgactat cattttaagg aatggtggtt ttcttgataa
    18961 attcattggg gatgcaataa tggctgtctt cggtattcca tttaatgaag aaaaacatgc
    19021 agaaatcgcg gttaatgtcg caattgaaat gcaaaaaaaa ctaaaggaat taaatgaaag
    19081 taaatctttt ggcgaattcc aattagccat gacaattgga attcacactg gtaatattat
    19141 agcagggaat cttggttcaa aagaaagaat ggattatacc gtaatcgggg atacagtcaa
    19201 tctagcttca agaatacagt ctttaaataa atcttattca agcgatatac ttatttctga
    19261 ctcaacgtat aaatcattga taggctcttc aaatattttt gcaagagaaa ttgatacagt
    19321 gaaagtaaag gggcgtaata agccatgtat actttatgaa atcctgcata ataaaaatat
    19381 tcattccttt aaccacaaaa tcaaaacttt ggagactttt tcatcagcaa tccttttata
    19441 caaaagtggt gattttatct cctccaaaaa tttatttctt acgcttattg ataatgataa
    19501 ttatgatttg ctccccgcaa tttacattga aagaattaat actctattaa aaaatccgcc
    19561 ggaaaattgg tcaggaatat atgaacatac aaataaataa atggcataat aaatacatac
    19621 taagtgaaaa atagaaataa caaaataaac cgaatggaga gatttaaaat gggaacacta
    19681 atgataggcg ttatggtatt aatgatgggt ggtatgattc tcatgcatac agcaatgatg
    19741 aatcatgatt ctaaagatca taaggaagga caacatgaaa cagcaaaaaa aactactgaa
    19801 tcgaaagcta aaacttcaga ggagttagaa acaaagtctt ctgaaaaaga acacaaacat
    19861 tagatgtaaa ttgcctaata atttaggaaa aaaattaagg agaagagtaa tgaaaaactc
    19921 aaaaataaca ataagtgtat tgctatatat cgtaactagt atagcaattt tttctcagga
    19981 tgtcccaaag attaaagcgg aacatttaaa aatggttgcg aagtatgaag agcaagtaaa
    20041 ggcgcaagga atgattgctg aagaacattc aaagatgaaa aaagagtatg taaaaaaata
    20101 tttcattaat gaaaagttaa gtaatagatc tacgattaat gaaatggaaa aacattgcga
    20161 taagattata gaagaagcga atcaattgaa aaagaatttt caaatgatgg ctgattttca
    20221 taaatctctt gctgctgaat tagataagaa gtaaggttta tattttcata aaccaatcac
    20281 caaaagtata tgactactct tttaaaattg agctttactt ttggtgatta aggtagctta
    20341 gattttttga atataggaaa ttatttagtc agttttgata gaggctggct attttttgaa
    20401 aatgatacca cgaagatttg atagtaattt tctcttagga gtttattgta atttcatgat
    20461 ttacttgtaa aggaatcacc taataatttt aatgtaatta gcttaaagaa attactagaa
    20521 aaaggagctg attcaaagaa agacacagag tcttttaaga aggcaattcc gattctagaa
    20581 aaattaggac agtcaaaagc attagatgaa aaattaaatc tatatgctga gttggtagaa
    20641 attttatcac ctactgtcgg acatcatgac aagtctggag ccaatttgtt ttattgccca
    20701 atggtaaaaa agaaatggat tgcaaaaggg gatgagataa caaaccctta tgataaatcg
    20761 atgcgaaatt gtggagaaaa attataatgt cctaatatat taggaaaata aaggagaata
    20821 gaatgaaaat ttcaaaacta atcattacat taattttaag cttaggcgta actgttgcag
    20881 ttgttgctaa cgacaaagaa gctaagaagt aatgtaaaat tagcgaccaa acattctatg
    20941 ttggttcaaa acccaattgt cagtaaaaac tggcagttgg gttttttatt ttagctttct
    21001 tatatactca ccaaaaaccc agccttctgc taagctacaa tattttctgc cgtaaaaatc
    21061 ttgttctggt tttacataat accaatgatt tgtaaacttc cccacttgtg ttttatcaac
    21121 ggtttttgca attacataaa gacgataatt cttatgtaaa acatttcgcc tttctatctg
    21181 tgtttctgtt tcagaatagt agcaatttat tggctcaaag tttactcccg gaccttgtct
    21241 aaaaaagaca ttgtctgttg tctcataatc attaggatat ataatttcat gaagaactct
    21301 accaataatt accctatctc ctgtgttacg tttttgttct atactgtaat aaaggttact
    21361 gttgcatttt aatgcaaaaa gagaatacaa tgattctgga gtttctacaa tattacaggt
    21421 tgtgaattca gattccgaga atgggctaca attttcagtt ttttttctaa atagttgaat
    21481 cgtattattg atttgtttat attttccttt aataaggcaa ctcccttctc cagcaaaaaa
    21541 ctgttcatat tcttcctttt tataaaaagt aaagttccag cctggagcat tgaattcagg
    21601 tttccatttc ccattgcctg caaaaataac agtagatttt tttaattgaa gatcttcctt
    21661 ttttgcaggt attggatttt gatccgtaga cttgatagag caggaaaaag cattgattaa
    21721 caaaacaagg ataataaatt tcataacgcc tgtattcttc ttatctagcc aatccatcca
    21781 ttgccatcaa tttaaaatgc cataaaatag aattgagcca attattattc atttctattt
    21841 cgaatcaaaa aataaatcta aaaacaattt gcttttttag ttaagcatct atcttatgtc
    21901 tcctagagga attattatgt cagacaagat tgaaatccaa atgccaactt tttatccgtt
    21961 tatgtttgaa atgccaattc gaaaaacaga tgttaccttg gatatacatg tatcctttgc
    22021 tacaatccta gatatagtat ttgaaggtca tatgcgattt tttaagttca tggggcacga
    22081 tgtaacggat gtatatggac gtagtttaat tttcgcaaat gcagttatcc tctataaagg
    22141 agaacttctg gcggatgacg tcgttcgcat tgaagtgaga gcagataatt tcctagaaaa
    22201 aggttgtgat ctattctttc atctcacaaa ggatggtggt aaaattccag tagctgatgt
    22261 aaagattcga atgttattct ttgactatac cattcgaaaa gtagcacttg ttccggaaga
    22321 gttcaaagca aaatgtctgg ctacactcaa aaatgatcct acacaacaaa aggcaattcc
    22381 aaaaacaaat gaatccccac tttggaataa agcacaccaa tttgtcctaa atctttataa
    22441 aagcacgaat caatttccca aagaagagat ggagcattta atcccgcgta tccgcaaatc
    22501 tgcaacctcc ctacccctat caattttaga agtaacaaat cgaaaagaaa agtcagacat
    22561 tgtaaaatcc tttggaaaga cgagaggctt cattgaagaa ctaagatatt atttaatttt
    22621 agcaaatgat ttaaattatc taaatgcatc gaatctaatc aaagaattag atgacttaaa
    22681 tacagagctt agagaaaatt acaaaaaaac aattagcgca ttatgaggat ttgccaatga
    22741 attcatttga aggaaaagta gccattgtca caggaggaac ttctggactc ggaagatcga
    22801 ttgcagttga gctagcaaaa cgcggagcaa aagttgcttt ttgtggaaga agagatcaag
    22861 agggaaacga tacactctca attatagaaa aatcaggtgg tgcaggattc tttcaaaagg
    22921 tagatataac caaagcaaag gatattgaag attttacaca gaatgtaata aatcggtttg
    22981 gtaaattaga tattgcaatc aataatgcag ggatcatagg aaaagcatct atctctacaa
    23041 aatacgagga agaagagttt caaaaagtct atgaaactaa cgttaaagga gtttggcttt
    23101 ctatgaaata tcaaattcca gaaatgctaa aaaccggaaa aggtgctatc gtaaatattt
    23161 cctctgtctc aggtttaatc ggttttcctt ttaatgcagc ctattctgca agtaaacatg
    23221 cagtcattgg tctcactaaa agtgcttctc tagaattttc acacaaggga attcgaatca
    23281 atacgatttg tcctggtgga attgaaacag atatgctaga aaatattttt gaatctacag
    23341 gaaaaccaga tttagcaaaa tcgaatatga caaatcttca ctcgatgcgt agacttgcaa
    23401 agccaaatga aattgcgaat gctgcgatat ggctttgctc agaagaagcc tcatttatta
    23461 ctggaattgc catgccagta gatggtggct ggacttctca atagataaaa tctttttagt
    23521 cctcttcaac tttaaataga tatgcttcac ggaatcaact ttagcatatt attcgtcgaa
    23581 aataatagga gagaaaatga tttgctttta cttatgttaa ataataacat gagaaagtag
    23641 agagaggctc tcttcctatt gggacataag aggatgatat gaaacgcaat tataaattga
    23701 ttcttagttt tttaatgatt ctgctgacag tgttttttgt agaagcatgc agaaaaaaaa
    23761 atctaccagc ggagtttgaa ctgatttcat ctaggtttgg tgaattcaag atgtatgacc
    23821 aaatgctaga tggtaagatt gtaaaaagaa ttatacataa agaagatgat gtttaccaat
    23881 ctgaaaactt acaattggtt caactcaacc aaattattca attgaataca tcggatatgt
    23941 ttttatttac agttgagtcg ttagacgaca atactcaatg ccctgcaaag tttttagtta
    24001 tcacattaaa ctcagaagga aaatttaaaa catttcctga aatcggaaat tgcaatgatc
    24061 taccagaagt agatgacaac ggggaacaag ttacatttac atttaattca attctatcat
    24121 acgaagcagt tacctatcgt tttgaaaatg gagatctaaa acaaatcgaa aatcaaaaga
    24181 gcgcagcaag aattatcaat aagtagcaaa ttatgaaaac tctatccact agcattcagg
    24241 ggctagacac ctcccctatt cgcaaagcat ttgaattagc ggcaacactt gagaatccaa
    24301 tcaatttatc gattggacaa cctcattttg cctgtccaca aaatatcata gaagctctta
    24361 caaaagcggc taacgacgga aagacagctt atactttaac aggaggaatt cctgaattaa
    24421 aagaagctct cgttagaaaa tatgaaacag taaacaagat caaatatgca acaccctctc
    24481 gtattttagt tacatcagga atcagttccg ccttactttt gcttttttca gcacttgtaa
    24541 atcctggcga cgaatgttta attatctctc cttatttttt aatgtatcca tccatgttaa
    24601 aatttcatgg agcaaagatt catcttctag atgaaaaatt tacgaaagaa gatacaaaga
    24661 aattaaaatt ttctaattta aagttaatca tctactcaaa tccttccaat ccaaccggtt
    24721 atgtaatgac caaagagcaa ttagaagcac tggcagattt ggcagaagtg aatgatgctt
    24781 atttaatctc cgatgagatt tatgaactat ttgattacga taaaaaattt ctatctgtag
    24841 gatctttcta tgagaaagca ataacgctct ctggattttc gaaaacctac agtatgactg
    24901 gacttcgtct tgcctccatt ctcgcctctg aggaaattac aaaagtttta acaacactcc
    24961 aacaatatac gattgtgtgt gctccttctg tgacccaata cgcaggaatc gaagcattaa
    25021 agactgatat gagttcttat ataaaagaat acaaagaaaa cagagactat gtatacgatt
    25081 cactcaaaga ttattacgaa cttgataaga gtgcgggagc tttttatttc tttttaaaag
    25141 tgaaaggaaa tgatgaagag tttgtcaaaa aagcagtttt agaaaagaag ctaatcactg
    25201 ttccaggatt tatttttaat aatagtcata gctatattcg aattagtttt gccaatacaa
    25261 aggaaaattt gactcgaggg atcaacgcac taaaagaact tgcttagtat tttctttatc
    25321 tatttttacg gattctggat tgcaagttca atcgcaagtt tttatactac gttaggcgag
    25381 aggattttaa aatactatta tagtcctctc agaaaaaaat actcgttttt tgaaaaatgg
    25441 aaaatcattt ttacaaaacc aagtgcctgt gaaaattgtg gaactcaagt caaagcaatc
    25501 tacctaacgc ctattcttgg atatttattc acaaagggaa aatgtccctc ttgcgaaatt
    25561 aaaatcgctt cgcattatcc aattctagaa tttctattcg gaattctatt ttgtattttt
    25621 ttaagtctca caaataatat tttattttct ctatctgttc tttttttatt cggacatgtt
    25681 ttagtctcga tgattacaga ttataaaaaa ttttcactcg attatgaaaa cttacctttc
    25741 cttctttttt tcggtgttct ttctaatttt cttttttaca atcaatttcc tgatttcatt
    25801 gattatgcag tcttcacagg ctttgttgca ttctatattc tactttgggt cttttataaa
    25861 aaaggaattg ggcttggaga tgtaatcttt gcacctgtct atgcttttct tgccggtcat
    25921 ccttattgga tactcttttt aaactcatct tatatactgg cagtgctttg ggctttttta
    25981 tctagaaaga aaggagagaa gttagacgga aaaccaattc ctatgggatt cttctttgga
    26041 attggcttac taatttcctt tttaggaaaa gcaatcatgg ctcgttagga aggaaaggac
    26101 tactaccgat agagacgtta gacaccgatg gatcttattt caatttctag cggctaacgc
    26161 gtaatttcca tccaactttt cccaaaatcg tctccgatta tattttagga gtgaaatgtt
    26221 ttttaataaa aatagaatgt gaatctttca tgaaagattt tgaattagat gcggtaactt
    26281 ttcttgatat acattaaatt tcttttagct ttcccgtaac gtatttgtgt aagatactcg
    26341 aaacgagagt ttgataaggc atgccttctt ctaaggcttt caccttgagt aattgtaaat
    26401 ctcttttatg aattcgtata ttcattcttg catcttttat aagagtagcg ctcgctgctt
    26461 tttttaattt taaattccat tctttcaaat ttttcacaga cttccattca ccggcttcaa
    26521 tcgattccaa taattctttt tcattttttt tcattcttcg cacctatata ttgatttgta
    26581 aatttcctac tagggtaaat cgttttaaaa aaatattctt cctttttcat atctacaacc
    26641 gttgggacta catatatgta atcatccaaa ttaacaagga gtaaaacctg attcggatat
    26701 ctttctttgt tcgtgtgttc aagaacatct actattttat ttccttcgat tgctattaca
    26761 attttctcaa acgaaatatc tcgactctgc tttaattcat cattctttcc attatcccaa
    26821 ttgaagacca tttttttttt acttggtatt ataatgtaat caatgtgtgc cttttgtcaa
    26881 tacaaaataa tctttgatag tgagattttg aagtaggtgg aacaaatttc tttatagaaa
    26941 cttattcatt gaattatata aaaggaaaca gaatatttta agcgacaaag ttttatgttt
    27001 tctatgaatg gattggagtt cgaaaatatt ttggttagat atgaaagaat acatcctttt
    27061 tctaaaagat aaatccattt taagtgcgtt agttgtttta atactctttg aactatttct
    27121 acaggctggc ttttataaga gttttttaaa aaagaattcc tatgcttcga atgtaaacag
    27181 aattaccgac cattcccttg gcaaacaatc agagcttgat ccagatattt taatcgtggg
    27241 gacatctctt gcttatgaag gtctatcgat gcaagcttta aatgaaagac taaaaccatt
    27301 gaacttaaaa gcccaaagta tcgcaatccc gggtgctgag ctcattgtcc aaggtctagc
    27361 actagaaaaa gtccttaata aatttaagcg ggtaaagtat attatccacg tgaatgaaat
    27421 cgaaatgccc tggacacttg gaattgtttt ctctcaagca acattagcta tggcaggaga
    27481 attagatagg aaacgtgcca tagaaaaatt ctatgaagat gaatataata tcagttatgc
    27541 ggatatcgca tatattttga taagactttg ggcatacaga aaagaccttg gagaatttat
    27601 tctaaatcct gaaaaaagaa ttaaagactt gggcaaagca aaaaaagcct tcaaagataa
    27661 tctatatagc tatgaaaaca attaccaaga aagtttaagt ctctatcatt ttaaaaatgt
    27721 aaaagaatgt cttacgatta ctaaggataa taccaatata ccacaaggtt ccaatccata
    27781 tcatagagaa gctattgcaa aaacttgcaa acttgccgag gaaagcgttt tatctttgga
    27841 ggaaaatggc tcgaccgagc tttatcagag acgactgaag aatttttata aaattttacg
    27901 agatagaaat attcaagtga ttaacgtttt tccacctgtt tccaattact tagaagactt
    27961 tcaatatggg aagcgtgttt tgttttggaa agataaatac aaagatgttt taggagaaaa
    28021 gatttttgat ctcacagaaa taatcccgaa ggaagataac gcaaattact tttatgatat
    28081 cgttcatgta aatcaaaaag ggatgagagt ttttacagat agactcggcg aatccttgtt
    28141 agcctatctc aaggaaaagg aattacgtta atgctattta attctttaca cttcctgatt
    28201 tatcttccaa tcgttttagt tctatctaat attttaaaag atagaaacca aaagatattt
    28261 ttactcctag ctagctttta tttttatatg gcttggaata taaatttcat ctggctattg
    28321 ttattctcaa tcgttatcga ttattttgct gccatttcca tttcgagatt agaaaagaca
    28381 gacaagagaa gaaagcatta tttgatttta tctctagtta cgaacttagg atttttaagt
    28441 tattttaaat atacaaactt ttttctagga gctataaatg accttaattt tattaactct
    28501 tttcgattcg tagagtataa tatcattttg ccggtgggga tttcttttta tacatttcag
    28561 tctatgagtt atacaatcga tgtttaccga ggagtcatgg aagcaaagaa atctttcatc
    28621 gactttgctc tttacgtatc ctttttccct caattggtag cgggtccgat tgtgcgcgcg
    28681 ggaacatttt ttagagactt agaagcaagg ctcattgtaa acctagacgt cattcgccac
    28741 tcatttgcac aaatcctcct tggatttaca aaaaaagtgg tctttgcaga taatctggct
    28801 atcgcggtag atagagtttt tacgaactat gcttctatga atgcgtttga aatttggaca
    28861 ggtgttttcg catttatgtg gcaaatctat ttcgactttg cgggttacac ggatattgcg
    28921 ataggagttg ctagactctt tggatttcaa tttgacatca actttcactt tccattcaac
    28981 gtaacaagca ttagccagca ctggtctaga tggcatcttt ctttttctac ttggataaga
    29041 gattatattt ttatacctct cggtggttcc aaggtttcta gactcagaac tcatatcaat
    29101 gtatttatta cctttttatt tgcgggtatt tggcacggag cggcgtatca ttttgtagtc
    29161 tggggaattt ggcattccgc catgctatcg gtagaacgag agtatgccca gacaagagtg
    29221 agtaaatttc taaacgaaaa aggaggattt gtctatagtt tattctgtcg aatttttact
    29281 atgatttgtc taggaattgg tcttcccctc tttcgagcca agacgttaac cgatgcattt
    29341 gggatgctaa aaacaatgtt attcctgaac tcgaattcta ttacttcgtc ttttagtaat
    29401 tacggttact tcaatttagt cgcacttcta tttgtaacag gatatatatt tgaaaaatac
    29461 aatttaaaga agatgctaga aagtaaaaga gcgtttaccc tgtttatcct tgcaaatgtg
    29521 tttatgctac tttgctatgg agtaagtgaa gcacagagtt ttatttactt tgctttttag
    29581 aattgcagga aagaatagcg atagaatgtt ttttctttta tcttttaatt ctagcattta
    29641 tcgctaaatg tgcatccaag gttttttctc atttcgttat cggtattaaa cctttctatt
    29701 ttgatttcac aaaaactgac attagatcaa ttaattcatt tttttaagat caatttttcc
    29761 caagaggtcc ttgactctat ttaaatttac aggtttaggc agataaaaat caaaacctag
    29821 atttaaaaac ttttcttcac ttccttccag aacatgtgcg gtagttgcta tggtaaaaaa
    29881 ctttctattt ttataagctt ctatcttttt catttgacta aaacactcaa ttccatctag
    29941 ctctggcatt tctatatcca taaagattac atcatagtta tttttctttg ctttctctaa
    30001 tgcttcatat ccatcttttg cttcatcagg taaaatattt tgtttcttca aaattgcgct
    30061 aagcaaaata acattgactt cattatcatc aacaattaga aacttcaacg aactcggtga
    30121 atttaatacc tctgtttgct gattatcaga agaaattaca gactcccctt taaagtattg
    30181 agtgactatt ctattttctt ttgttgaaaa tagattccac gcgttttgta aagaattaag
    30241 caaaatactt cctgatattg gtttcacaag ataagcagaa aaaccggctt tttgaatttc
    30301 atttagagaa tcaggataat ctaccgaaga caataaaatc atacacaaat catctaactc
    30361 tctatacttt ctaacattcc tagctagctc aattccattc atttcaggca tattataatc
    30421 aatgatagcc atttgataag gatcacctga agcctttgct tctaataaag cgcctatcgc
    30481 catctgacca gatgcgaact gaccattcct catctcccaa ctatttacaa aggaatgcac
    30541 aatttttctt ccaattaatg tatcatctac aattagagtt tttaatccat aaatagaatt
    30601 tatgtttttc atctccgtgg aattaagagt atattctaaa tatatttcaa aaacaaaaat
    30661 tgaacctttt ccttctgtgc gaggcaaata ataaacatcc ctccaagtaa ttctgcaata
    30721 cttttactaa tcactaaacc aagtccagtt ccattgtatt tcctttgtgt tgacatatca
    30781 ataggcgtaa actctttaaa taaatctcct atcctttctt ttggaatgcc aatccctgta
    30841 tcctcaactt caaatttata ttttaattta ttcaaatcgt aatcgttcag ggtagaaact
    30901 ctaatattta catatccagt atctgtaaac ttaaccgcat tgcttatgag gttaaataag
    30961 atttgcctca ctctataagc atcagtctta acttgataag gtatagtttc cggaaaatca
    31021 aggcggactg aaattttttt ctgttttgct tcattaaaaa aaacatttaa aatttcttgt
    31081 atgattgtat gaagattgca atcaatcatt tgaatctcca ttttcccagc ttgtagttta
    31141 gaaaaatcta aaatatcatt gattagattc attagatgat ttgcagaaga aatcaaattt
    31201 tgaacatagc ttctctgatt tgtatctagt ttggtttctt ctaaaagatt tgcaaaacca
    31261 atgactccat tcagtggagt gcgaatttca tgactcatat tcgataaaaa acttgttcta
    31321 gagtgaatcg caatttcggc tttttcaatt gcctccctta gctgtttagt tcgcaaactc
    31381 acaaatttgt ttactgtttg ataccagttt agaaacttaa gaaaggcaat gaataggatg
    31441 aggctaacag taacttgcca gaatatttct ttttcaaata tacttttctc ttttatttta
    31501 atccatttct ccaagatttt atctttctct ggttttgtta aagaagataa agatttatct
    31561 aatatcttcg aaaaaatagc aatgtcttga atcacaccaa atcgaaattc atatttaaag
    31621 tctacatcac cagaaataaa aatatttgaa aaaagttctc tttcatagaa ataagaaatt
    31681 ctagatattt cagagacagt tgcatctact tctccacttg ctacttttgc gagtgcattc
    31741 acttcatctt ttacaggaat tatttttatt tgtggatagt tcgtttgaat gtatttataa
    31801 acagcgctgc tttccactaa agcgattttc tttccaatta aagattttag atttgggaag
    31861 tctcgccctt ttctagacac aattttattt gtgagagtta tatacggctc tgtaaatttt
    31921 aaaaattcct ttctatcatc tgtttcttgc actgcaaaaa ctaaatcaat tttcttttct
    31981 ctagcctggt ttaaaacctc aacccatgaa tctatataaa tgaattcgaa ttggattcca
    32041 attttttttt caatcttttg taaatattga attgtaattc catcatatcc atcatcacca
    32101 tcggttgttt taaaggcgag tggcggatac tcatgaatac ccactcgtag tttggggttt
    32161 gccttgaccc atttttcttc ctcagttgta agaatgaaac catctgcttc gatgacgaat
    32221 tggattccta ggaaaataga aataaaaaag aaaaatagac ttgttttttt gcgcttcatt
    32281 taattgcctt tttgctcata tccattttat agatggaaga tacatctcat cttataatgt
    32341 gaaaaatctc tattattttt ttttttaaac aaaccttaat gaaagatttt atctatcttg
    32401 tatcaaaata tcttttgaaa aataaattat taatctaaaa acgatacaca ttgtaaccag
    32461 agtaaataat ctcactagat aatcatagca tattcttcat taggttaata taatatttat
    32521 ctaatctttt gaaactgtaa gcagtcaatt ttactaggta aacaattcat ttagcatttc
    32581 ttttctcaga atctaaaaaa tttttaaatc gaagttgaac taaacctaaa aagtattatt
    32641 cgttcttttg atttagcagt tttcgattta cttaaatcaa tttctatcga attggattga
    32701 ttaaaaacta gatgaaatta ttttcttttt tttaaaaact cataagcatc ttttgcttct
    32761 atgaattttt tattcatgag ttcataaatt tctggcttag cattgtgaaa cttatcagga
    32821 tgatatagga ttgccatttg cttccaagct ttctctaatt cttctaatgt ataattttca
    32881 tttagattaa ataatgtaag tgcatttttg aatttaacag aatctccatt ctcattgtac
    32941 gaataagaat aactcgacga acttctattg gctcttgcat tcacattata acgagcagtt
    33001 ctcttgatgt tttgaacata cgcgccgggc aaggaaaatt gaaaacaaag tcggttaaat
    33061 aattcttctt ctgtttgcgt tacctcccca tccgcaagcg ctacttcata aatcattaga
    33121 aaaagcatgg ttgtaagttc aatgcgtgct tcccaaccgg caatactcaa atgttgaaag
    33181 taaagattta caatgtctac aagtgaatca aaaatttgtt ctttagaaaa ctcttgaata
    33241 tgttgctttg tgtatgaaac tacttgtgca atcagagttt cttctacatt attttggaat
    33301 tctctatgta tagcttgtcg tataacggat atttcttttt ctgaaatgcg accatctgat
    33361 ttggcgatat gaatacaagc agaagctaat atcgctgcat attgatttaa aatagaatag
    33421 tttctgatct catttctaat tttataaaca agagaattta catactctct attatcaatg
    33481 atcgctggtc cactaaactc atccaacatt ctcttaatag caagcacaac tagaaagact
    33541 cctaaaaaga aaaaaagaaa ttctctagaa tttagaatta agaaaattcc gatcagtgtg
    33601 agaatgagat ttgtcattga agggattaaa tactataata ctttctataa aaatatagta
    33661 atggtctttt agattcgtct gtataagcat attctacttt accaacaaag atagaatgat
    33721 ctccgccttc ataaactgtt tccaatttac aatcaagatg acaaagtgag ttctttaata
    33781 aaggagactt tgtaacctgt gtagaaaaac cattttcaag aacgatttta tttcgatccg
    33841 tatctggttt tgcaaattgg ttggaaagag tttcttgttc agcagaaaga atgttaatcg
    33901 catactctcg aatttgaatc agtttatcat gacttgcaga attctttgct acagaaaata
    33961 aaactaaaaa aggatccata gagagggaag taaaactaga agcagtcaga ccggaataaa
    34021 ttccattgtc tgaataggtg actatagtaa cgccagatgc ccaatgtccc atagcctttt
    34081 taaaatcatc ttttgtaatt gccataggta aatcctactg gtaggaaaga atagtcaaat
    34141 gaaaaataga attcagattt agttttttag aaatgagata taacgtgcaa tcaattcctc
    34201 attggccaaa attggataag aagtaggaaa tatatttgta tttttaaaat caaattctct
    34261 atctgttttc tgaaataaat caagcggata aatagaatta aacgcatcat ctgaaataaa
    34321 tactctcgca atcaataaat aaaaataact ttcttcttta tctaaattga gtatatattc
    34381 taaaaattca tttttagaga tttgcttgat tggtatttct aaacgcttca aatattctaa
    34441 taaatcataa attgtaagtg attttaggtt tgcaatgtga aagattctag aattactttg
    34501 actcttagca atcgatataa atcgataagc ggcatagtca agaggagtca tatccacttt
    34561 taaatctaaa tcatctaatt ttggtaaaat cctatatttt aaaataaatt ctaaaaattg
    34621 agtaaagaaa tcttccttct tagaaaatcc actcttagaa tctccagtta tcaaaccgaa
    34681 acgataaata cgtataaaat cacatccaga ttcgattgca tttaaaagaa tcttttcact
    34741 tacccacttg ctctctgcgt accctccata aacttctgag gaataatcga ttctatcttc
    34801 tgaaaaaact tttcgtggat catttgatga aacaagtaca gaaagagtgg aagcatagtg
    34861 taaatatttt ctaatccctc tcaaacaaaa aaataagact tctaaggttc ctttcacgtt
    34921 tacatctttt aatttttggt atggtagaat tagatttact tgggctgcca aatgaaagac
    34981 tgtatctatt ttattggtta aagattcata taaatcaagg ttcattccaa aatttttctg
    35041 agatacatcc ccttctatca aatagatttg atctaagtct atctcaagtc cttgcttccc
    35101 aagataaaac tcaatttgat tgtaagcatt ttttctattg cgaacaatac agtagagatt
    35161 taattctgaa tcagcggcta attcaaataa gactctagaa cctagataac cagttgcacc
    35221 cgttagtaga atatttttcc aatttttaga atcttttttt ctggtctgga ttaaatctag
    35281 taagtcatct ggaagttctg cttccctctt taaaaactct acttttcttt caatgacagg
    35341 ttcttttaaa atagataata attctcgaat ggttggattt aaaatccatt tatcaattgg
    35401 aaaatgaatt ccaattagac tacactccgt gacaaaagat agcaaagaaa gagaatctcc
    35461 ccctaataga aagaaatcat cctccactcc aattggttta caagaaagaa gttcttctag
    35521 aatggaagtt agttttattt ctatcgcatt tttaggggga gatatttctt ttagataaaa
    35581 aggtctactc tcaatcattt tagaaaatga attaaaatcc attttctcat tctgatttag
    35641 tggaatttga tctaataaaa taaattgatc cggaaccatc cacttaggca attcaataga
    35701 tagacttttt tttaattcta gaaaataatt ttgatcaagt ataaaatctt tctgtaaaac
    35761 taaataagct aataattttt tttcctttgc actaacataa gaaaaaacga tttccttgcg
    35821 atgcgttaat atcttttcaa tttcaattgg ttcaattaaa tttcctttcc atttaaactg
    35881 tcgatccatt cttcctaaaa attcaatttc ctcatcagaa tgatagcaaa ccaagtctcc
    35941 tgttttataa attcgctctc cgttgtaggt tataaatttt tcatttgtaa gtttttcttg
    36001 attgatataa caagtagcaa gtcccactcc acctaagtaa agctctcctt tctctcctct
    36061 atctactggc tttaaatctg catctaagat ataaaaagtt cgattcggaa ttggaattcc
    36121 tattagcggt ttactccaat tatcctcgca tactataaga cttgagcaga cagtagcttc
    36181 cgtcggacca taaacattta caattctaaa tttacttgcc caaagcctaa ctgtatcaac
    36241 aggacaaacc tctccaccaa tgataatagt ttcaagactc tctggaattt cttcaatggg
    36301 taaaatggga aggagtgcgg gtgggaggca aatatgtgtt atcctttcat ttgctagaat
    36361 tgaaactaag ctttctagat ttttaatttg attgtcctct ttaatagcaa gctcggcacc
    36421 ggaaaggagt gaaatcccaa tctccgaaag agaagcatca aatccgatcg ataaatactg
    36481 caaaacctta ctagaagatt ttactcgaaa aacgtccatc tgcgtatcta aaaaatttaa
    36541 aatcccatcc tgcggaagta agaccccttt tggatttcca gttgatccag aagtgtagat
    36601 aatataagct cctttcatat ttttagattt ttctttctca gaaatttgaa tcagcttagt
    36661 agctagattt tcgattgata aggattcatt ctgtgtgatg aattctaaat tgtctgttag
    36721 aacccaacta gcctctgcat catttaaaat ccagcttact cttttgagtg gagtcttttc
    36781 gtctattggt ataaaaatac aaccaaggcg gaggcaagcc aaaagagaaa gaatgtagtc
    36841 tgaagatttt ttcatccata aagcaattcg atctccctct ttgactccaa tttcttctaa
    36901 tttatggcta atattgatta gcctcatttc tagtttttta aatgaaagaa tttgattttg
    36961 atcagataaa gcaatattct caggatattg atttacaact tctttgagtc gtaaaagaaa
    37021 agaggatttc aatctacttc tgctccaaat cttcgcatca tttcgactaa ttgtttctct
    37081 acatctctac gaaaaattcc atgtgtaaat gctacatttt cttttttaat ctttgcgaca
    37141 aatccagaag aatcattcgt aactgtataa tgaattttac ttgttcctag tttttcaaat
    37201 tgaacataca tcccgccctc ttcctcgtga aaactcacaa ttccggctaa ttttgtttta
    37261 ttaattccct tttgcatctt ctctacaaaa tggcgcaaat cgccagatga tttgattaat
    37321 ttcattttcc ttccccctaa ctacacaatt agaaaataag gaaaatatct tgcaacaata
    37381 tctagagaac aaaaacctaa gaacatgcag tatgtattta tttttatagg gggtggactt
    37441 gggagtgtaa ccagattttt tatttctgaa cttaccaacc gaatattagg aaaccatttc
    37501 ccgcatggaa ctctcatggt aaatgtaatt ggctctttta tcattggtat tttatttgga
    37561 atttatgaat ctagactcag cacgattcca gtctttcgtc agattgtctt tattggattt
    37621 ctaggaggct tcactacttt ttcttctttt agtctagata cattgaattt atttttacaa
    37681 acaaaatttc tgtctgctat cttaaatgtt tttttaaatc taagtctttg cctaatcgct
    37741 gttagcctcg gatatacagt cctctctttt ttaaataaag gataaacaat gatttcaaga
    37801 actgttttaa ttttatctct agtgagtttt tttactgata tctcttcaga gatgctttat
    37861 ccaattctac cactctatct aaaaagtatt ggattttcca ttgtatggat tggagtcttg
    37921 gaagggattg ctgaggcaat ggctggactc agtaaaggct attttggtag ggtttccgac
    37981 aaaatcggaa agagacttcc ttttgtgcaa ctaggttatg ccctcagtgc aatcgcaaag
    38041 cctgcgttag ttctaatctc tcatcctatt tggattttat tttctcgctc tgtcgaaaga
    38101 cttggaaaag gaattcgcac cggtgcaaga gatgcaatgc tttcatctga aacttcaaaa
    38161 gaaaacaaag caaagatttt tggatttcat agaggggctg atacactcgg agcctgtctt
    38221 ggtcctgcaa ttggtctttg ctatctttat ctttatccag aagattataa gaatctattt
    38281 ttatatgcat tcattcctgg aattcttgca atctttttta ccttttttct aaaagagagg
    38341 caaacactta gtaaccatac aaagtatcaa aactcgattg catttttaag agaaagtcca
    38401 aacctttata aaaaaatatt aatcggtcta gttttatttg caatatttaa cagttcagat
    38461 gtattcttac tccttcgaat caaagaaagt gggctttctg atttagagat gattttagtt
    38521 tatattttct ataacctcat ttatgcaatt cttgcttatc cgatgggata tattgcagat
    38581 cggtttggaa tcaaaaagac ttttgtattt gggcttttta tttttagttt agtttatttt
    38641 ggaatgagcc aaaaaggttc gatcatttac tatctatttt tatttaccgg ttactctctg
    38701 tatgccgcaa gcacagaagg aattgcaaaa gcatggattg caaacatttg tccaagagat
    38761 actgccactg caataggaat ttattctggg cttgttagta ttgccttatt atttgctagt
    38821 tcgattgcag gatttctttg gtatcaattt ggagcagaat ctgtttttta cttctcttct
    38881 cttggtagtc ttatgatttg tatttatttt tttagaatcg aggagtaaat gacttgcagt
    38941 agaagtattt tagaaagtta ggtttttagt atgaacacag cagagaaaat tttaaatatc
    39001 ttcaaagaaa ataacattca agccaacgaa aaattacata aaagaattgt aatggataca
    39061 attaaaagct ggggactgga tgttgttgaa atcagaaatg catggcacgc actcatgggc
    39121 tacggactcc ttatgcaagt aggagatgaa cttgttctaa ccaaacaagg ggaaagtgtc
    39181 gcttataaaa atcaatgaaa gacaaagaag aatataaaat ttttttcgaa tctcatgatg
    39241 ctttaggtaa ggtctttggt gctaaattta tatccatcac agaagaggaa tgtatctatg
    39301 aatatgaagt ttcaaagaat catttcaatc cgaatggaac gttacacggc ggagccttgt
    39361 tttctgtaat ggacagttgt caaggagcat tctttcacta tatcgtagat gatatttata
    39421 aatttggggc aacagggact gcatcgattc gttatttagc tcctatcacc ggtgggaaaa
    39481 taaaaatcaa aacttggctc aaagaaaaag taagaagaag atttatttta ctctctactg
    39541 cctatgatga atccggaaag gaagtggcaa cattagaaga aatctggatt ggaattttaa
    39601 agtaaaatca atctgaatta aaatattttc accacgaaga tcacgaattt cacgaaggta
    39661 agagcttaac atttatttta ttcgtatctc gactccactc gataagcgct atcgtataga
    39721 aaggatcgat ataatggaaa tcctccatct gtgcccatcc gtgttcaaac tcaatgttgc
    39781 ttactatggg tcgcaacata atgtggtaat ctttttctct tttataccga acgctaaaat
    39841 taatttccca tttttcaatt acaaataagt gttcggtcta aacacttcct agtgttccca
    39901 cacttcagta tgtgcggtat agcaaacgct actccaataa aatccttgcg ggaagtttct
    39961 tttgacgttt ggtatataat cgtttcgtta tcggtgtcat cggtggtaaa aatattttta
    40021 cgttctatca aagtgcaatc ctccatctgt gtccatccgt gtttatccgt ggtaaaatct
    40081 cttccgccat ttaatttttc caagtaaaat cgaaatagaa aataacgtta actgcaagag
    40141 taaaggtgat atttttggat caattggaat atactttctc acaaatggag aaagaaaaag
    40201 tccaagtgta acaaagacac tccaataaat ctgccaaaag ggaagaaaag taactcgaat
    40261 ctgtaagata aaaaataaga gaaaagcaat gattaaagca ggaatttcag ggtaaaaaat
    40321 aattccaata gaaacaagaa tcgctgcgaa taccaaataa agatcaatat tccttccaga
    40381 aatttttatt ttcgatgtag aatatacatc ttcaaaattg gaaatggcaa gaagagaagc
    40441 gactgaaaat ccaattgcag aaaaagattt ccaaccaaag agtaaataga gataataacc
    40501 tgttgttgga gctaagagga atgtcgtata aatttctaga aaaaagattt cttttttatc
    40561 acgagataaa aacaaatcaa acccaacaat caaaaggacg agtaaggcta cattttgtaa
    40621 tctcgagcca tcgatttctc ctagataaat atgcagcagg aatagaagaa aaacaccagc
    40681 aaaccataat tccaatctga tagacggaat tcgaacaaaa atagcattag aaaaatgata
    40741 aaatagttgg atgattaaat caaaaatagg agcaaatagg attgtaaaga ggataacacc
    40801 tataaaccgc aatagaattt caaagaatga agtagcacta ctttgtaaag aagcaatagc
    40861 aaaaacttct gaaaaaacaa tcgttcccaa aatcgcaaat accaatccga ttaaaccgcg
    40921 attttttttc ccatcaaaat caatggataa aaacaatcga taaaaaaaac aaatgtaact
    40981 atagataatt tctcgtagcg atttacccca aaagaaaaat agagcaggaa tcacggtaaa
    41041 attcaagaac acacttccat ttttataaac tcctgcggct aacattggta atccaatgaa
    41101 tgcaagaatc catattttac tcatttcctt ttttgcattc atccaataat ctacaatatt
    41161 tgaatcgatc tcataatcaa ataattctgg acatttagcc tgagtatgct cgatatattt
    41221 ttgaatatta agaatctgca atcgactcgt tctgatatta cttggatctg atggaatggg
    41281 ctctagcaaa ggagctaatc ttttattttt aagaatttct ttttctagtc taattctttc
    41341 tcttctagct gaaatttttc tctttataat tgtgagagaa taatatttat actcttcata
    41401 atcattccat tcaaaatagg aaaaaacttt ttctaagaat cgaaatggaa acttttcgtc
    41461 atttggcatt ccatcactat agagcaattc taattcattt cgaaggctta aattaaattc
    41521 ttctaaaaaa ccaaatgtat attcttttaa ttcattctcg agatgattcc attttataac
    41581 tgccttctcc tcaccctctt cttttaatag gtttatcaat tctctaacaa ttcgattcgc
    41641 ctcaattgta ttctgataat cgattttatt ttttaaagag ggaaatacat cctgttcaaa
    41701 ctcatcctct tcttcttgat aagtatcatt tttctgcttc gaataatatt tttcttcttg
    41761 cggatattca ctgttatcct ttaaatcttc gatttcttcc ttgtgttcaa tggatattgc
    41821 ttctaaattg tcttcctcct cagttagatc ttcccattcc caatcttgtt ttgtataggc
    41881 aagggcttct tcaaaggctt ttcgaagagt ttgaaattct tcgggagatt tttctcgatc
    41941 aatcgttttt aaaatagccg cataggcttt tttaattgcc ctttcctctt tggttgggtc
    42001 aatctttaaa acagaccaga tattcattag aacagattgt ttccttctaa atcatttaga
    42061 acatatacaa ttctttcttt cgcctttcca attttttctt cgtcttgtga atctaacgct
    42121 gaaataaaaa gagtcatttc ttctccaatg tatcttctga tattcccgag agattcttca
    42181 taaagtcttt ctgcgcgaga aagtaatgct cgattttctt ctttctccct cgggtggatt
    42241 tttaaatttg agagactttt tagtttttct tcaatctctt ttggtgttaa aactcctgga
    42301 ttttcttcaa tcacagatct atatttctcg cctgacgcat gaacatggac ttctacttct
    42361 aaaattccat tgatatcata ggtaaatctc acatcggcgg aggcttcact tgcaggaagg
    42421 ggtgggaaac gaacggctac ttcccctagg aaaatattat cttttacaag ccttgcctct
    42481 ccttgataaa tcctgactcg cattaccttc tggttatcct ccacacaatt cacaacttcc
    42541 gatcgactga ctggaacgat tgtatttctc tcaataatag gcatatagaa cccactcttg
    42601 taatcatttc ctactctttc actcacttca attcccaaag tatagggaca aacatctgtt
    42661 aaaacgattt ctttcaaagc agagtttttc atcttaagac cagcttgaac accagcgcct
    42721 aacgctacaa tttcatctgg atttaaacta ctggcaggaa gaatcccgaa cattttcgca
    42781 acaagttttt ttatcgaagg catacgagtt gcgccacctg ctaatacaat ttgattcaaa
    42841 tcactcgctt taatctttga atctcgaaga gctctctcga ctggttttct aagtcttgcc
    42901 aagagtggct ctgtccattc cgaaaactca tctgaagtga ttggcatggt ataggtagtg
    42961 tccttccaat ttactttcat ctctacacga ttcgattctg ttaatgctct tttggcaatt
    43021 tctgcttggt tacggataat tccgattagc ttctgatctt cttctggtaa tttttctttt
    43081 ctaacaactc tctctacaaa ccgatctaca atacagtttc taaaatcttc tccacctagg
    43141 aaattatctc ccgtgctagc tcttacttcc atcactcctt caaataaatc taaaatagat
    43201 acatcaaaag ttcctccacc caaatcaaag actagaaatt tactttcatc ggctaactcg
    43261 tgtaagccgt aagcgagggc tgcggcggtt ggttcattta aaagaccttc taccgaaaga
    43321 ccggctaatt ctcccgcaat tcgagtggct tttctctggg catcactgaa ataagcggga
    43381 actgttatga ttgctttctc aattttttcc cctaaaaata cttctgcatc tcgcttgaga
    43441 gatctcaata ctaaagagga taattcctct gccctgtatt tttttcgtcc taagaaaatt
    43501 tctttattcg ttcccatata acgtttaaac acagaagcag tcaaattgga atgagtagaa
    43561 agtctttccc tagcagataa accaactaat acttcatctt tttcagtcat actcacaaca
    43621 gaaggagtta gaaaagaacc aagtgaattc ggtattaaaa cagatttacc atcttgaaaa
    43681 acagaaacca agctatttgt cgtgccaaga tcaattccaa taatcataaa tgaataaaaa
    43741 ctctaagcta aaatagctta caagtctttt tgttttttaa aaggtaacta ctagagcctg
    43801 tcgatcaatg ctgtcaactt aagataaatg aaaaagaagt aaccacgaag aaaagagaag
    43861 gtgaactact acatatttag tggacacgat tttaggcaaa ttgcttctca tattg
//
LOCUS       DAOQOS010000039        43558 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_343_length_43558_cov_6.373239, whole genome shotgun sequence.
ACCESSION   DAOQOS010000039 DAOQOS010000000
VERSION     DAOQOS010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 43558)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 43558)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43558
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_343_length_43558_cov_6.373239"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1724)
                     /locus_tag="PLF66_10465"
     CDS             complement(<1..1724)
                     /locus_tag="PLF66_10465"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HMZ64508.1"
                     /translation="MNFYGQVFSIQEELFRGSSYVISRGIQNSDQKPIIIKYLNSEIQ
                     EGNENLKLKNEFYIHSQRKLNFSIKAVDFIDDTFLILEDFKGISLSDFIKNRKLDVTD
                     FLFIALAMTECLIEIHENNIIHKDIKPANFIINENTKELKLTDFGISTLFQKEYVSSS
                     EIKLEGTIQYISPEQTGRTGKPIDYRTDLYSLGISFYEMLTGRLPFATNDINQILHFH
                     LAQIPDSPKKYNPDIPTILAALIQKLISKDPIARYQSANGLKFDLIEIQSLFEKNEEI
                     LSSMPFQLGANDFSDKFIISEKLYGREKEIQILQESFLDTLSIHQSNRFLLITGYSGI
                     GKSSLISELNIPIQEKKGFLISGKFEQYQRNIPFFAISQSFADFIKQLLSREKSEIGI
                     WKRDIQTAVGSIGKVITDVIPELELLIGVQEDVPEVSTEQGMNRFSYAFLQFVKACAK
                     LEHPIVLFLDDLQWADSGSLFLLEQLLYEPDLYYILIIGAYRNNEVNSSSSFHFFLSN
                     LEKENYQFKTIHLGNLEKKAIRDLLVDTLHSKSESIEELSDLIYSKTGGNPFFTKQFL
                     FNLQDESF"
     gene            2387..3067
                     /locus_tag="PLF66_10470"
     CDS             2387..3067
                     /locus_tag="PLF66_10470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64509.1"
                     /translation="MLRLKLRRNKNLFRKFILIGAKSFIAGIFFGIGIFTLDLLAVAI
                     SGTFNNFVAGQIIKANDLNTNFSSLRTAIESIPDFTKSGGNAVLSSGGLILNTNTLAN
                     SSTLTVNGRISARNLGIYCGTTSATNGNAGGYLTVKNSCATICGNTNAHMCTAHEMSL
                     SRQLGISITEPSWFSSYGYWLGTVAINVYDCNGWTSASNSQAGTINSSGGPSWNTCDN
                     SLPIACCL"
     gene            complement(3461..3871)
                     /locus_tag="PLF66_10475"
     CDS             complement(3461..3871)
                     /locus_tag="PLF66_10475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64510.1"
                     /translation="MLSVLKFGLWAQATCLILGMILHTSAVITGHKYFYEKIFIPETD
                     VVFFILISLGGTLAFLGQVRNRYKNIKLWIGSLFVSIYFLISIPVHAKTMITWSTDHF
                     KIFPEIYSLFILPIQGIFLLIVFFELKYLKEGSH"
     gene            complement(3865..4281)
                     /locus_tag="PLF66_10480"
     CDS             complement(3865..4281)
                     /locus_tag="PLF66_10480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64511.1"
                     /translation="MITGQRILRFHGILLCITGSLLYMATIVGKLYNLGMFAFLAGNP
                     VASIGFQEAYALISILGVSIYLGSNLNDRFHLHILAAFIHVFLIYINISHWSFYTKLN
                     IQIAGYISTSAHFLLMTLESYFAMFTIKIQKGDLRC"
     gene            complement(4278..4718)
                     /locus_tag="PLF66_10485"
     CDS             complement(4278..4718)
                     /locus_tag="PLF66_10485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64512.1"
                     /translation="MKQSNLKSISRWILRIHGILLILLAFGNTFFSWNAMNSNLEGPF
                     HFLFSNHAAEIGLLQAYLLMSLLGGVLIYGSLQSEFMIFDFLGIAAHLIPLVTLFVLK
                     STIIANMGQETILYSASIHCSFILVESFSILIQISTSKFIGVAA"
     gene            4873..5457
                     /locus_tag="PLF66_10490"
     CDS             4873..5457
                     /locus_tag="PLF66_10490"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HMZ64513.1"
                     /translation="MNDRYRVKEEIIERAYQKLLSSGITETTANYLADISGVSKRTFY
                     KYFPSVDEFLNQLMDYIKNQVHNEFASVLQSSNKNYLLSVKSLFKNLPKAYGKNFENF
                     MIHLHKQRPDLIKSFLDFRKNEFDLILEKLINLPEVNLKKRKLNKRVTRDILINLFEK
                     MATPYYIAETGESMDTVVHTISEIFFHGIITENE"
     gene            complement(5482..6903)
                     /locus_tag="PLF66_10495"
     CDS             complement(5482..6903)
                     /locus_tag="PLF66_10495"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HMZ64514.1"
                     /translation="MEELKREIEDLKGIIASYETLHELSQRELADARQAIKGYEAMLN
                     LSAEERKQSYETVDAFRLVQEVSRQELIDASKTIQNILSMDKELSVVTDVKPLMTKIL
                     KSAIQSLSADLGIIFLKKQGDLVPVFFENITENQITILKYIKSLVKNSLHLEGGKDKG
                     DYVVKHDNEEKTIHYVCNSFKQDERVIGVIYLEKSANEVPFHTTDLNTLEVFSLQVSI
                     AFQQSSSNIKIISMLREINQKNQILERAQELNVRLSQTKDAFIKNLSYELKTPLSFIH
                     GFSELLQGTEPMNIEQIRSHVESIYLESEKLTDLLNDLLLITNLETEIKLKKENLDIK
                     QIVDLCLSKVIEIIKRKKLEVNIAVAGEVFADRSLFTRVLMNIIKNAVFYNKRDGKLD
                     IFSEKLKNEIRITVKDTGIGISEESQNFIFDKFYRAVNVDSYEGIGVGLFIAKKVIDL
                     HGGSISVTSELMKGSTFQIRIPQ"
     gene            7376..8770
                     /locus_tag="PLF66_10500"
     CDS             7376..8770
                     /locus_tag="PLF66_10500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64515.1"
                     /translation="MELKKKHILRLFKIGIIYSLLTFCSQSVYENPCNPQSKILQKNL
                     LLKFLLDDKSPYCGQASSNFGRNLGNAAKELTSFSFLSPPITGDITEKKITLTLPFGT
                     NATNLVAKFTTTGSKVSVNGVTQTSGITANNFTFPVSYIVTGIDGSTETYTITTTIQS
                     NPGKAYYSKLTTGSERYGNAETLNGSTIQSFTPVSTFMLTAVIDSDRDILYVGYGAAG
                     ENVIRVYENASVNTDTVTRTITVTPNTRIGGLAIDSINDRLYSVNFFTTASSNVNRFI
                     FRINNASSRNGTLTAVSDFAQISSTALHPSSQSIRRIALDSKRDILYICDYGANSVFA
                     YHGVSTYIHGSLWEPSRIITSVPQPAGITIDTKNNILFISSFSGNSIHVFDSGDTTNG
                     TTGANRIITSGSLNGSSGLHFVYPFNRLYVASLNAGRILYFNDARNINGNINPDGISG
                     AISSPTDVSIDINR"
     gene            8952..9569
                     /locus_tag="PLF66_10505"
     CDS             8952..9569
                     /locus_tag="PLF66_10505"
                     /inference="COORDINATES: protein motif:HMM:NF021576.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2306 domain-containing protein"
                     /protein_id="HMZ64516.1"
                     /translation="MKKKSEWFLLIGLIVLVIVPTLAGIVRIFNLGSAEAFQPENIRF
                     TKSPISITLHVVSSILFSILGIFQIIPSFRIPNLKWHRRIGRVLLPIGFISSLTGLYM
                     TQFYPKVETDGPTLYFIRLLVGFVMTLFLILAVYTVLNKKFRDHGNWMIRAYALGLGA
                     STQVITHLPWLITTGQLPTGLTRDMAMGAGWLINLAIVEWRIRKT"
     gene            9839..11416
                     /locus_tag="PLF66_10510"
     CDS             9839..11416
                     /locus_tag="PLF66_10510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64517.1"
                     /translation="MNIQRKFAISIMLLFFISISYLIPVVSVFLVYGKETGILNTSYF
                     NLLLPFTVTFSWGILLLLFYTKYKTNISKIQYLILALLITVSFLSFTGSPAFRYIKYT
                     GIFAFIILIFIWVETLIQSQIIKNLSRYIMISLSYILVFLSILEPALIFPQIGKLENL
                     NPFFKKEGFIDFLGLFDNTRILEGGYLKQNIHSNYLIGDGSLGKININSKGFRSKWEL
                     ENPKPKDEFRILLAGDSFTVGYRIDDAYTLGGLIESELNNKNIKTFTAGLFEQYSILQ
                     WQKQYPKQFDADVLLVGICLGNDLVQTYSKLKSIELGYSFKYHPVNDPYYNQVLEKEK
                     LPLTALDSKSSFNYNVWNPATSIRKDLRIVELVKLYRPTGIWTMPLPGSIPTWDPMNG
                     FGAFLKNSEKTYSELFQYFGNTLFEIHKNASNAGIKVHFVIYPMRFQQSEAEWKATKE
                     LYGLDSSGFNLENPNQIIANICNQYQMSCLDLLPLFKENGKEILHFPFDQHWNKKGNL
                     IAGKYIANFIKEQHFRK"
     gene            complement(11695..14244)
                     /locus_tag="PLF66_10515"
     CDS             complement(11695..14244)
                     /locus_tag="PLF66_10515"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HMZ64518.1"
                     /translation="MDSQTYQQFLLKGGIKEEEIPSIFKQGRFMVFLEGVAGFLIYLI
                     VKENPSQFGHSESIDFLEFLLPSLEGSLLVINWATLHFALLFIRFLLFFILGNKNRFV
                     TNLQKISTFLAGFYWGISFLLGANGFDKTNLILLSLIILFVISGWCVTTVSFRYTLLF
                     AIPAFLPVILTLFLYPSYTSRIYYPEVYLLFFISVIFAAYKIGKNHDVLYRLAVNITR
                     EKEETEIAQKALFESECEYRNLVQNTNGIIIRWDSSGKILFMNAYALSFFGYEENEII
                     GKNILDTILPEKDSAGRDMQDMINSFLKNPESFADNENENKLKDGSIVTIRWRNSISF
                     NKTILSVGVDITENVIAREELKKREQELSSILEAIPMPVVITHSTGNHYLYVNEKAIN
                     MFKVPREKVSQVRVPNFVVDPPSRDAYIQKLRQNPEKVVTAELLLRDFEGREFWTIIS
                     YAMFNFRGEMAILAGIQDITEQKRVEEILSSARKQAEESNQLKDRFISLVSHDLRGPI
                     GGIQTMSEMLISPEYKDTIQKEGSIKFLTMINKTSTGLIELLNQLLNITRLKTGRIIP
                     IKKHLDAKTLVENIHMTLNVKMEEMGIHFENEIESGKSIYADPHLLGEVVSNLLTNAI
                     KFTPKGGTIFVGMEKEDEDYTYLWIKDSGTGIPPAILPDIFKHEVKTTLVGLHGEKGT
                     GLGLPYCKDIIDAHGGSIDVSSDKNKGTCFKIILPKIRPSILIVDDLEAHRIIAKDVF
                     LKSDIWVDILEAGNGNQALKILENVKPDIIITDINMPEKNGWEFIFELRSESKWKDVP
                     IFVMSADLSESNDFENKQIELDIKEFITKPIRAEELISKVNPYLKKQVILT"
     gene            complement(14751..15374)
                     /locus_tag="PLF66_10520"
     CDS             complement(14751..15374)
                     /locus_tag="PLF66_10520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64519.1"
                     /translation="MIVSSFAIWNIDFKHSSLSSDNRIWKLPSKELKEVILYYFLFAI
                     LLFVFVAGGFVVTTEFLNGLSDIFHWNLVDAYRDAPEKFLFRLLVTIVTPLAFLGIYF
                     WPILLSLRSSFRIQAEAGKLFCGKKTFRLIDDAKMQLRKMRHVTRHGSGIAKIRKAHI
                     LVEWKVEDKKGDSHLIVFSEESFDELSNFFQHVARVSGIPLDLVTNP"
     gene            complement(15371..15844)
                     /locus_tag="PLF66_10525"
     CDS             complement(15371..15844)
                     /locus_tag="PLF66_10525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64520.1"
                     /translation="MSQISTKIAIAHILFGILVIGVCLDFQYSKEFYLDGKWLNALLN
                     FFLFSLPSTESSIYEWFFALLITIPWNLFVFLIYLFLLILSVILLNVLSVLLILLPGI
                     PSILGGILHLLRWKPALKILKVCTYFYVFVFPIGIVFTYWCLHEFEKIQERNSKS"
     gene            complement(15841..16371)
                     /locus_tag="PLF66_10530"
     CDS             complement(15841..16371)
                     /locus_tag="PLF66_10530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64521.1"
                     /translation="MNFEVFRFNLFSGEVENLFGVNIGFNNKVNTIFGLQFGFLNDLK
                     RGVAMQLGILNFSDTNKKILKLGIVNILFPFDKRLENNQVPLGDKKKSDVDHINSGLQ
                     RDSNFGLSLGIVNISDSFNIGLLNIQEGESAFSLGVFNYGISGYQLGVLNYCSKSFLP
                     YMIIINFCNAKDKDNL"
     gene            16697..17404
                     /locus_tag="PLF66_10535"
     CDS             16697..17404
                     /locus_tag="PLF66_10535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64522.1"
                     /translation="MDILGFQIRERLGEQLPGGRLNSTIFFDENKNKYISRRPVRYID
                     FTVNGNSIASLIDADLNDIVGSMQLKQGEEQFFGISTHVLEILQFKFPFTLKYNRVPL
                     FVCPECGYDENCGSVTFELAETEDQVIWRHFGSGFETEATSFMKEFTWSALEEEDFEF
                     WIECGKSNAILEKFKGSRYEWILGGKPSTCEIDSQIDLTNLEYPEIGPFTFKKKDYFN
                     VLSEIESTLKKYRLSEN"
     gene            complement(17430..17717)
                     /locus_tag="PLF66_10540"
     CDS             complement(17430..17717)
                     /locus_tag="PLF66_10540"
                     /inference="COORDINATES: protein motif:HMM:NF018784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HMZ64523.1"
                     /translation="MKKYILILILFLNQCATLLSIPSWYKKNTAVFYSGTRCDVNLLI
                     ENDSGKFDPIYLIDLPFSFLLDTVLLPISIPFALVMVRHHGEIGFAPPCVE"
     gene            complement(17809..19308)
                     /locus_tag="PLF66_10545"
     CDS             complement(17809..19308)
                     /locus_tag="PLF66_10545"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HMZ64524.1"
                     /translation="MLANLSLQNINFSYTPAQENLFNSLNLHFTNGWTGIVGQNGTGK
                     STLAKIISGKLKPNSGQIKYPLEENRILMTENSFSRIDGLDEFWEDRFSGYYRSLLKI
                     KDHWIYSWEELSFGERKRIQIAVSLWINPDIWILDEPSNHLDISGKRILLEALETFKG
                     IGILISHDRDLLDALCNKCIFLRKGEVSIYSGNYSESLTQERESQKSREVKYKIAKNN
                     LERAKRSIQSLKEKEFSHSNSLSKKNIDRLDHDTKGKIDRARLSGKDKKGSRKKVVLE
                     NKKVELEKETNVLYKKVHRMEGFQFQSTPIYRDILFHFTEQDWKPNSEISIFIPELYL
                     KPNSKIGIQGENGTGKSSFLKYLYERLNIPSSLVFYLPQEIDSLYWEKIQNEIRQKNK
                     EELGKIYQVIYQLGSIPEQVFYSKDPSPGEKRKVLFGLGAIQKPAVLILDEPTNHLDL
                     PSVHCLEDALSEYDGCIVLVSHDEKFLDSLIKTKWIFERNQKNQIYIRK"
     gene            19768..20298
                     /locus_tag="PLF66_10550"
     CDS             19768..20298
                     /locus_tag="PLF66_10550"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HMZ64525.1"
                     /translation="MKFLKNLFLPKIQKAILENDFNKIENSEINEYFSNGSFPLEFAI
                     EQDKWESAKYLLDNKADPDKRIHIDEQTLVMSIIQNNYNLKYLNLLIEYEANLNLKDK
                     NGYSAVIHCILTGHLEALKILLENQADPNLQDKDGATALNHAKSTGGLSELIDLLLEY
                     GADPNIKDRHGKTYNM"
     gene            complement(20441..22573)
                     /locus_tag="PLF66_10555"
     CDS             complement(20441..22573)
                     /locus_tag="PLF66_10555"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HMZ64526.1"
                     /translation="MKINKKLIKIPISWQVFLFTLITTLTSIIVTLFIYGSYTQKLIE
                     DEIVNNYLKQSDAILTGINPVLNDYKIKINDCIKNNLCDPSYIEEVPLSKRKDLTPEQ
                     VFYEKENDIKLIYNESSGRKFLISIDIVHTLLIEISLKFSEEIYLINAKGIVLLNITG
                     NVPFGKSILLEKETLSQLKAKLTQGAFYLKENDITSLYVFRIIPELDLYLVYGKAQSK
                     VFSDINKVTDFAIIYFSISIPFTILLSLGFSHLIKKRISKFLVRIKRITSGNYSERLD
                     TNSIYYYDEYVELADAFNRMVSSLEKFHSMNVEKILEMNEELEILVKELREAKQLADS
                     ANKTKSLFLANMSHEIRTPMNGILGTIQIIESSSLDEEQMKLISMIKTSANNLLAIIN
                     DILDTSKIEAGKIELEEISYDVKKLLVEIVDSMRIQAKEKGNEIELIISDSFPEYILG
                     DQVRIRQILLNLMSNANKFTDRGKIFLEAKVLEEGDPFLLSFLVRDTGIGISEDKIEK
                     IFESFSQADASTTRKYGGTGLGLSISKKLIHLMGGELKVKSKVGEGSEFSFQIIAKKG
                     FAIAKTLSVQNFEKIKWRDDLKILVAEDDPINQRVLSKLLLKINLKVKMVDNGYKVLE
                     ELDSTLYDLIFMDVQMPLMDGITTTKEIRKKFSLQSPIIIALTANAMKEDQQICLGAG
                     MNDFLTKPYFIEDLQRILLKWIGDESRT"
     gene            complement(22570..23775)
                     /locus_tag="PLF66_10560"
     CDS             complement(22570..23775)
                     /locus_tag="PLF66_10560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64527.1"
                     /translation="MILIKFVKYNIFLTIALFLILFFIGKSFAEDSFILLEWEPNEGA
                     SQYLVEISNTKNFSTIVYSTRINETSLRINYNDKYKYGRVAGIDKIGATGNFSKTFEI
                     QPRIVIPKALPETVKPIAISEPVPIPEVEPVYYNLSDIISLESSDRESEIFYQLNSSP
                     WRRYMTPFYLDKDGLNEVYYYSINKLGKKELIQKKTYLVDSSEPEIHLGIKHYFVKED
                     NVYLTGPNTVITVNIVDLISGVKSWNAQFEYEDKIIPLNREEIIYIPAHLNDKRVFLV
                     VTAEDKKGNISKKSISFIHDSAPPEIQVIMPNLINRLYTNVGSEIKIAAKDSGIGLLY
                     IHCSINNSEYRDCINPLTLNNPGEFIIKIKAADWYGNAREITLDKIIVLEKEKINTNI
                     HIQNINYIK"
     gene            complement(23778..24539)
                     /locus_tag="PLF66_10565"
     CDS             complement(23778..24539)
                     /locus_tag="PLF66_10565"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HMZ64528.1"
                     /translation="MNRFLLSTVFLISISIFSEDIEEYVIKDKDTLSKIAKEKLSDMN
                     KWKELLQYNQIANPNMIRPGVVLKIPAYLSKKPKIEPIAGLKVFLGEVQYKQNELDWK
                     NVEKNQKFLENDVVRTKEKSSAEIEYLEQPLTVIQVREKSIIKLEKASERSLDLRLGN
                     IFLKVLNQNETDKIKYRVKTPSSVASVRGTEFYVSVDKQETTLLGCYKGLVDVNAQNI
                     TVKVPAGYGTKVVKGQPPLEPFKLLDEIIPKPVKE"
     gene            complement(24988..25530)
                     /locus_tag="PLF66_10570"
     CDS             complement(24988..25530)
                     /locus_tag="PLF66_10570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64529.1"
                     /translation="MNKLFSCHIKKFCKKSFFYLVLMIHFIGCAGFYNQPQMYEKQNP
                     NLTSKSIRLEFTGFYFYEKEKKLLKKEILESGFMEDSNSSLLLEVILEEREHTYSHRF
                     LHFTNMVASLFTLGIIPFYTITEHSITYRYFEGKKEIAKTKQILRLDQFRGLSMLPLT
                     FFFWPSSAFDRSILDSWKLQ"
     gene            complement(25523..26131)
                     /locus_tag="PLF66_10575"
     CDS             complement(25523..26131)
                     /locus_tag="PLF66_10575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64530.1"
                     /translation="MLNSILFLIPFLFFIHCSTFLLGERPTVNPKVSKFEKDISYELI
                     GWKGKENRIKATSILDTFEKSKKFKSLSYANSTNSEWKIQIVLDESPQLAILLGEPVQ
                     PVSWVAEKRPGRYTLYLLNRVLSAATRLVLPIFQVREERITFRIWKDNLKKGEYIYPV
                     ETIRAIGWISFILMFYDDKKEIESIYSDYTLKFLNDSETLYE"
     gene            26387..28336
                     /locus_tag="PLF66_10580"
     CDS             26387..28336
                     /locus_tag="PLF66_10580"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HMZ64531.1"
                     /translation="MFKFILILLFSIQLSNSIFALEPILLEKNKEEYSIGKYLEILED
                     QTRTKTFEEIQKIDTKVWRKSETDTPNIGHTISAYWFRANIDYPLDKEMEYFLEIAYP
                     VLDKIDLYFLEEGNQNYSHLILGDKFLYKERIVSFYQFVFPLKLNPKSKYQIYIRIET
                     QGNLLVPITIYNLNSFLKRVNSSMIAYGIYFGCILVMIFYNFFLYLSIRDRSYLQYVF
                     YLMSFFILQAGVTGIGLAHIWSNYPIFNDGISIYSSLLGITILGFTSSFLRTKDFFPR
                     MHRLTKISQWIFGISILLYFILGFSITIRLVLLLGLITILIAYSIGISVWRKGFKPAK
                     YYFFAWSFFLFGGTLYILKTLSLLPSNFLTEYGAQIGSIFEFILLSLGLANRINLEKE
                     ARQKAEKLALENQHRAEKFKEELEARKRAIELEESRDRLSIIGQSTASIVHDLKNPIA
                     TIRAYTELSMGDEISETDRKEYLEMVLREIERLGDMAFEILDFTKTKINLSLTETNLN
                     DFLEDIYKFLKLDFDYAGIDFKVKAEDTIILNIDRERIRRVLINLANNAREAMAERTN
                     SQFSIQCFKDGNEIVLSVADNGNGLPDRVIENIFQAFVTEGKKNGTGLGLYMAKIILE
                     AHGGKITYETGKSIGTTFYLRFPFC"
     gene            complement(28397..30460)
                     /locus_tag="PLF66_10585"
     CDS             complement(28397..30460)
                     /locus_tag="PLF66_10585"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HMZ64532.1"
                     /translation="MKKNIIVLFLILLSFCNQTSNSNPPSVKKGVLDLRSLTSLGKVN
                     LDGDWEFYWNCQLASNPQSCVSRENKYFAQVPGIWNGLVIDGKEISGQGYASYRILLY
                     LKETDTPISIRFLDAATSYNLWVNGKLILSNGRAGTDKESTEPRFLPITKELTNLKQE
                     NEIIVEISNFHHYKGGFWESVTIGDSEDLKIHRENKVWMDVFLSGSISIMLVYHIGLY
                     ILRRKDISSIHFALFCFVVMLRLLVTGERLLFYKFPNFNWELGNKIEYGTLYLVIPTF
                     AAFIMSIFPNEFSNLFKKIFISISLVLVALLFVTDISVYSYTALPWEIFIILVCIYGL
                     YCLVLSVIHNRDGALASLVGFLFLISTVINDILYANTVINTAYLLPYGLFLFIFAQSF
                     LISLRFSKAFLSVELLSENLQKTNEAYSRFVPTEFLSVLNKTSIIDVKLGDQIQREMT
                     VMFSDIRGFTSLSESMTPQENFNFINSYLKVMEPIISNHNGFIDKYIGDSIMALFPES
                     AEHALNASISMLRELRIYNEERIRKNLSPIKIGIGLNTGNLMLGTIGGKNRMDGTVIS
                     DSVNLASRLESLTKTLYIPILISETVYNHLQNKEQFSIREIDTVFVRGKSKPIVIYEC
                     FDADEEQVRICKKKNLENFSKGLIAFRENQFQKAKEYFIECEKKCPEDSIAFIYINRC
                     NEKLA"
     gene            complement(30650..31789)
                     /locus_tag="PLF66_10590"
     CDS             complement(30650..31789)
                     /locus_tag="PLF66_10590"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMZ64533.1"
                     /translation="MKTLSLFKRNLILQWRDYWSLLITISLGPFFVIIYWMISVGFPS
                     YYNVLIVNLDKGKYGDLFIQNSSSDERTKETVHIQKIESVEQGKSILKKRDADVLLVI
                     PENFSSEIETAISKLDTRPEVFFYGEKSNTRYLVGVITSFSIVDSFIKLQTKAKDPWT
                     MKEFFIDEEKVKRTDFELSIPGIFILSIIMLLFSAGMIIVRDIEDHTMNRLKLTKMNI
                     YDYAIAITLTQVLVGAVSVLLTLGLAEVLGFRMVGSFSSILIVFCITFIGIIGIAFII
                     VSFCRNSSVFVIVGQFPLFILIFFTGAMIPIPRNPIFYVLDHGVCWNDFLPATLAVTS
                     LNRIFNDGLSISEILTPVILLALISLFYFLIGITLFKKRHLDLGN"
     gene            complement(31786..32727)
                     /locus_tag="PLF66_10595"
     CDS             complement(31786..32727)
                     /locus_tag="PLF66_10595"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMZ64534.1"
                     /translation="MSTVVLETKNLRKEFGSFIAVKDLSIQVYEGEIFGFLGPNGAGK
                     STSIAMICGLLNPTSGSLYLQGKEILKSDKSLRVRVGICPQNIILWTRLTCLEQLLHI
                     AEMYEIDSSQAKTRALHLLEEMGLIDQKNKLAGTLSGGMQRRLNIILALMHDPEIIVL
                     DEPEAGLDPQSRILVREYIKSLAKIKTVILTIHNMDEAERLCDRVAIIDQGKLLSLDT
                     IENLKKKNRQEIVLELQFESEKIAIAANQVLSSRSSIKIQRFHNQCILSDPNVLEQLS
                     DILQVLKKNSLSIVEMKVRENSLEDIFIQLTGRRLRE"
     gene            33182..34447
                     /locus_tag="PLF66_10600"
     CDS             33182..34447
                     /locus_tag="PLF66_10600"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HMZ64535.1"
                     /translation="MLIVFFSKNAEMAQKIEQSLNNKVFLDFEFVFSKDPAETIHFLE
                     YSMPQFAIFDLDDTDFSNAKNFEIIAHDHWLSNTGIFGIKEDKTELSAYDFPFFYIFS
                     PSEIVSQMPKILSVLMRNENLLFRAGIIQDIGNKGIFVIQSETEDVKAYAELIASNLS
                     NRGLIQTQKKYAVIFALNEMLINAIEHGNCEITAEEKKAWIASDRKINELVDIKKKDP
                     KIATRVVTLEYELLESKILITITDKGKGFDYESFLKTDLLSVTGYSGRGILMTKCFID
                     SIQYHPPGNKVSLEISYDTANMKLPVGLEEHKIIDLSAGDILFQKGDKSNGLYFIVRG
                     EFEAWIDGKIVSILDRSDVFIGEMAFLLRHRRTATVKAKTNSQVIFIEAKEWVDTIRK
                     FPMYSVYLSRILARKLNNTSITVSSLKQI"
     gene            complement(34829..36496)
                     /locus_tag="PLF66_10605"
     CDS             complement(34829..36496)
                     /locus_tag="PLF66_10605"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HMZ64536.1"
                     /translation="MPHKSKNKSGLKKILILDKDLVSKEFLVKKILEKGYAIEEKIPF
                     ASLQFPPKLNPIDLVIVEEVDRENFLSSENYKKLKEKEIPVLFLSQNLFSMESDLIFD
                     ILLKPLREEEVVFRVERLIHISRDMSHLKKKNAEWEVDMKVQHLIENELLEKENRLAT
                     ILDSVEAYIYIKDSHYRYQYANKKVRDLFGETMEDIINKDDFDFFDYPTAINIRKNDR
                     LIIEKGQRIEEEEVNTGLNGTTNVFLSVKLPMYREDGTIYALSGISTDITKHKLAEEK
                     LKKTLAQLEEQHTQLKATQAQLVQAEKMAGLGTLVAGVAHEVNNPTNYISLSSRALER
                     DIDKFRKEVISLMAGSEDEIIQYFEENFSKFQKSLSNILEGSERIRTIVQDLRSFSRL
                     DEAEKKEADLCEVLDSTLRIVKTQYKRSIKITSHYLVKRKIECYPAQLGQVFLNILIN
                     ACHAILQKQIDTLEQFSGKIKLTLQENEESDELQVVIEDNGCGMSEDVLQRIFEPFFT
                     TKLVGQGTGLGMSISYGIIQKHNGRIDVISKMHEGTTVTVSIPFIRK"
     gene            complement(36510..37631)
                     /locus_tag="PLF66_10610"
     CDS             complement(36510..37631)
                     /locus_tag="PLF66_10610"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HMZ64537.1"
                     /translation="MKLVIDKEKLKEIQEKNHTEKKYTVLLVDDEPANIEALNMILED
                     KYNIIKAYTGMEALAFIKSQADPSRINLIISDQRMPGMTGVEFLKQTIPIIPKTVRMI
                     LTGFTDINDIIDSINEGQVYKFHTKPIEPKDLLVSIQRALEAYELEKQNIVLIEKLKE
                     MNENLEQLVRERTEQLEESMRTLQRQKEELEYLSNFQDTLVNELEVLSITDPLTGLAN
                     RRWLESFTQEECDKAIRKDKKISVLMLDIDHFKKYNDFYGHAMGDKCIKIVAESIKKN
                     MRKTDLAARYGGEEFCCVLPHTTQEEAIQIADNLIQTITKEAVPHEKSDTASIVTISV
                     GVFTTMPQDKSLWKELILNADANLYKAKQNGRNQIWFKG"
     gene            complement(37815..38930)
                     /locus_tag="PLF66_10615"
     CDS             complement(37815..38930)
                     /locus_tag="PLF66_10615"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HMZ64538.1"
                     /translation="MIYFDYNATTPIHPEIKKLIPEWLDLYANPSAVHTAGRQAREAL
                     ERARREILEDLDLEIYKLAFTSTGTEANFLAFNFLLQEEAEEGMQVLLSAIEHPSIYN
                     LKTLLKEKGYLVRWIPVDQNGRIDLEFLEEHCNEETVLVAVMLANNETGVIQPLKKVR
                     EITDQFGVYLHCDAIQAPGKMKFNFSEIDADSFSFSSHKVYGMKGVSAVAYRSKPVPI
                     FRGGDQEKGIRPGTENLLGILSFAKAISLIKENQKEYLNLQRENQKILESRLKSLGAK
                     VVGEKAERLSNTSCITLPISNEKLVIELDKRGFGVSRGSACHAGSWEPSSVLVAMGIS
                     KEEANQTVRISTGIFTTEKEIQSLCSAIQEILAKYFC"
     gene            complement(38937..39845)
                     /locus_tag="PLF66_10620"
     CDS             complement(38937..39845)
                     /locus_tag="PLF66_10620"
                     /inference="COORDINATES: protein motif:HMM:NF024580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4007 family protein"
                     /protein_id="HMZ64539.1"
                     /translation="MKLKLAGHETFYPREHWLYKGMNEKAAKIHAKNTRRVNEFFKES
                     EEANDEYPTDYMGVGINMVKSIRHWVNAFGLLEKDSPSDFISPILKRDRYLDKLATSW
                     ILHYQLIGSENPPTTWYWFFTHSDLVTFDKETFIRSYKEWIESLDIKQNYSEKSLEND
                     YQVLTGMYAKEENKDLFYPSQFANLGILSYNPHYKRYTRNANPAIDPHIVLYCMLMFR
                     DRFFSNSESLDLENIRNIPESPLRVFKIDIDYFYEIMERVTPKIAKKFKLSRTAGMKT
                     MQLNSLTKQDLLSDIYSIERQDNYLD"
     gene            complement(39847..40998)
                     /locus_tag="PLF66_10625"
     CDS             complement(39847..40998)
                     /locus_tag="PLF66_10625"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HMZ64540.1"
                     /translation="MQTYPRKAEKSIVFALENFPVVAILGPRQSGKSTLAKEILGRFP
                     NSIYLDLESTTDLARLQNPELFFESNANRLVCLDEIQHTPELFPVLRSIVDKNKKNAQ
                     ILILGSASPELLKQSSESLAGRIAFEYLTPFTILEVSGQTDFKLEEHWWKGGFPLSYL
                     KEDSGSNLWRENFLKTFIERDLFQFGIKTTSLQLTRFLTMMAHSNGQVVGKSKLAASL
                     GVSHNTISHYIDIFEQTFFLRSLEPYFTNLKKRTVKSPKIYFRDSGVLHSMLKIESFN
                     ELLGHPICGSSWESYCIENIHAVYKGYELNYYRTSSGNEIDLILTKGQRKIAVEFKIS
                     TAPKVEKGFWISLQDLEIDEAFIICPIQDSYPIEKNVSVSGLKTFLEKY"
     gene            complement(41045..41509)
                     /locus_tag="PLF66_10630"
     CDS             complement(41045..41509)
                     /locus_tag="PLF66_10630"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HMZ64541.1"
                     /translation="MIHLLPPYYNNLGKRLIKAPKLYFSDTGLLCSLLGVHSEADYKD
                     SVLNGPIWEKFVFTEILKTRFFKNGYNLFYYRDQNGVEIDFLLESAKEITLVEVKKAE
                     RPSEEKLNFHKVAPLFTKWKPKCILACPVPESRILRYKEYSVWNPVNVELSK"
     gene            complement(42022..42351)
                     /locus_tag="PLF66_10635"
     CDS             complement(42022..42351)
                     /locus_tag="PLF66_10635"
                     /inference="COORDINATES: protein motif:HMM:NF021873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
                     /protein_id="HMZ64542.1"
                     /translation="MANDNNPTELKPPRINKIFIDKQNIYVLFVDNTIKKYDIGPLLE
                     KKSFTKLKNLSYLKTAKVDSGGYGISWDDECDLSENELWTKGVIVKDREELAKLHLLF
                     VGMEKIF"
     gene            complement(42442..42744)
                     /locus_tag="PLF66_10640"
     CDS             complement(42442..42744)
                     /locus_tag="PLF66_10640"
                     /inference="COORDINATES: protein motif:HMM:NF026989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64543.1"
                     /translation="MAEHGNSNQNESSHHLYEIIDKVDNDVVKYGISGEELTPDGFSP
                     RAQRQVNAMNTFVKWIRFFARILVTNISGRSKAKEIEEEYIQNYYNANGRNPRGNL"
     gene            complement(42755..43339)
                     /locus_tag="PLF66_10645"
     CDS             complement(42755..43339)
                     /locus_tag="PLF66_10645"
                     /inference="COORDINATES: protein motif:HMM:NF025390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4240 domain-containing protein"
                     /protein_id="HMZ64544.1"
                     /translation="MLTVNGIFEKGQVRLSETPHVNSGTKVLVTFLEESSENKSDFTE
                     DDFWKIISLLDWKQSESKLQILPCINALTRFTVADIFQFEEILAEKLFLLDGKKFAMH
                     LGEFSYSKDKPFSGDLFLYMRCFAIASGKEHFQKVLANPSLLGSQSFESLLYIGERAY
                     RILTGQELSYVSKFSYETYSNFSAWEMEKPVSIY"
BASE COUNT        14683 a         7459 c         6475 g        14941 t
ORIGIN      
        1 ataaaggatt catcttgcag attaaataaa aactgcttag taaaaaatgg atttccccca
       61 gttttggaat agattaaatc tgagagttcc tctatcgatt cggattttga atgcaatgta
      121 tctactagta agtctctaat tgcctttttt tctaaattac ctagatgaat ggttttaaac
      181 tgataatttt ctttttctaa attactcaaa aaaaaatgaa aactactaga tgaattgact
      241 tcattgtttc tatatgcacc tatgatgagt atataataga gatctggctc atacaataat
      301 tgctctaata aaaataaaga accagaatct gcccattgca aatcatctaa gaataataca
      361 atcggatgct ctaattttgc acaagctttg acaaattgta agaaagcata ggagaatcga
      421 ttcattccct gttctgttga aacctctgga acatcttctt gcactccaat gagtaattca
      481 agttcgggta taacgtccgt aattactttt ccaatgcttc caactgcggt ttgtatgtct
      541 ctcttccaaa ttcctatttc agatttttct ctagagagta gttgttttat aaaatctgca
      601 aaggactgag aaattgcaaa aaatggaata tttctttggt attgctcaaa ttttcctgaa
      661 attagaaatc cttttttttc ttgaatggga atatttaact cactgatcaa tgaagatttc
      721 ccaataccag aatatcctgt tatgagtaaa aatcgatttg attgatgaat cgatagagta
      781 tctagaaaag attcttgtaa gatctgaatt tccttttctc ttccatataa cttttctgaa
      841 ataataaact tatctgaaaa atcgtttgcg ccaagttgaa acggcattga actcaaaatt
      901 tcttcatttt tttcaaatag actttgaatt tcaattaaat cgaatttgag tccattggca
      961 ctctggtatc ttgcaattgg atccttcgaa attaattttt gaattaatgc agcaagtatt
     1021 gtaggtatat cgggattata ctttttaggt gaatctggaa tttgcgctaa atgaaaatgt
     1081 aaaatctgat tgatatcgtt tgtggcaaat ggcaatctac ccgttaacat ttcatagaaa
     1141 ctaattccta aagagtatag atctgttctg tagtctatcg gtttcccagt cctacctgtt
     1201 tgctctggag aaatgtattg aatcgttccc tccaatttaa tttcagagga gctaacgtat
     1261 tctttttgaa ataaggtaga tattccaaaa tcagttaatt ttaattcttt tgtgttttca
     1321 ttgatgataa aatttgcggg tttgatatct ttatgaatta tattgttctc atgaatttca
     1381 atcaaacatt cagtcattgc taacgcaata aacagaaaat cagtcacgtc taattttcga
     1441 ttttttataa aatcagaaag agaaattccc ttaaaatcct ctaagataag aaaggtatca
     1501 tcaataaaat caactgcttt gatagaaaag tttagttttc tttgggaatg aatataaaat
     1561 tcatttttca gttttaaatt ttcatttcct tcttggattt cagaattaag atatttaatt
     1621 ataatcggtt tttgatcaga gttctgtatc cctctagaaa ttacataaga cgatcctcta
     1681 aataattctt cctgaataga gaaaacttga ccgtaaaaat tcatactcaa ctaatattta
     1741 ttttgattta aaaaaaagca atttatatta gagactaaaa atataaaatc attttgcttt
     1801 aaatattttt taaaaatcaa aaaagagctt attttttaat acctatccta tagtaaatta
     1861 caaacaatct atctctcttt ttttagtgtc aaggtctcct ctgctctcaa ttgttagtat
     1921 tttgaattta ttagcgttat ccgccctttt taaagtgaat gagagctcta ttcatttatc
     1981 tatatataat gattcaaata tataatttac gaacagagtt ccaagtagaa ctcaaaattc
     2041 atgatctgtt tttttccaat ttgaatctca gttcaacatc agaagaacaa attttcaaaa
     2101 tgcaattatt gtgtcctaat acaaaagtaa gaaggtaagt ttagagatta taatttattt
     2161 tattttcaga tttataaaga ataatgactt aaatttaacc ttatattaaa aagagtaaaa
     2221 aaaaaattaa aaagacacta aacaagtccg atcattccat ttttttttac ctacaaaaat
     2281 tagattaaaa gagatctaaa gatgctgtgg ttataaaaat aaactttgca aaaatggaat
     2341 ctacaattaa attacaagat cataaattag aacggggaaa tattttatgc tcagattaaa
     2401 attaagaaga aacaaaaatc tatttagaaa atttatccta ataggagcca aaagttttat
     2461 agctggaatt ttttttggga ttggaatttt cactctagat ttacttgcag ttgcaatatc
     2521 tggaactttt aataactttg tagcagggca aataatcaaa gcaaacgacc taaatacaaa
     2581 tttttcctct ttacgtactg cgatagaatc tatccctgat tttacaaaaa gtggaggcaa
     2641 tgcagtatta tccagtggtg gattaatctt aaacacaaac actcttgcta attcttccac
     2701 tttaacagta aatggacgaa tttctgcacg taatttagga atctactgcg gaactaccag
     2761 tgctaccaat ggaaatgccg gtggatacct taccgtaaaa aactcatgcg caacgatatg
     2821 tggaaacaca aatgcacata tgtgcacagc acatgagatg tctctttctc gccaattggg
     2881 aatttctatt acagaaccta gttggttttc ttcatatggt tactggttag gaacagtagc
     2941 tatcaatgtt tatgactgca atggatggac gagtgcctct aacagtcaag ctggaacaat
     3001 caatagttca ggaggaccaa gttggaatac ttgcgataac agtttaccaa tagcttgctg
     3061 tttgtaataa agatagaatc atctatagaa tactttcagt cgttttctta gaatttttta
     3121 gtgaggtttg gcttttttaa aaacttagat tctagaatct tataattata ctaattagcc
     3181 aattattttt aaaaataagt ttttcaactt tgatggataa accaattaga gtttttagaa
     3241 atcagaaact taaatgaatc gtaaaaggaa atctttttct gaatgtttat ttattagcca
     3301 cgttatgcat aaacacgtat attgagagat agagtctatc gtaaatacat gactcttgtt
     3361 gaatgaatca atgctaaccg atttattgat tcattcaaag accatactaa aataatatct
     3421 tttattatac gtatccattt ctatattttg caaaattcaa ttaatgagag ccctccttta
     3481 aatatttcaa ctcaaagaat acaataagta aaaagatacc ttggattggc aaaataaata
     3541 agctataaat ttcgggaaat attttgaaat gatcggtaga ccatgtaatc attgtctttg
     3601 cgtgaacagg tatactaatt aaaaaataaa tagaaacaaa taaactacca atccataatt
     3661 taatattttt gtatcgattt ctaacttgtc ctagaaatgc taacgttcca ccaagagaaa
     3721 taagaataaa aaagaccaca tctgtttcag gtataaatat tttttcataa aaatatttat
     3781 gaccagtgat tacagcactt gtatgcaaga tcattccgag aattaaacag gttgcctgtg
     3841 cccacaatcc aaattttaat acacttagca tcttagatct cctttctgaa tttttattgt
     3901 aaacattgca aaataacttt ctaaagtcat taatagaaaa tgagcactcg ttgaaatata
     3961 tcctgctatt tgaatattta attttgtata gaaactccaa tgggaaatat ttatgtatat
     4021 cagaaataca tgtataaaag cagcgaggat atgtagatga aatctatcat tcagatttga
     4081 tcctaaataa atacttacac ctaaaattga aatgagtgcg taggcttctt gaaaacctat
     4141 agatgcaact ggattgcctg ccaaaaaagc aaacatacca agattataaa gttttcctac
     4201 gatagtcgcc atatacaaca agcttcccgt tatgcataaa agaattccat gaaaacgcaa
     4261 aattctttgt ccagtaatca tgctgcgact ccgatgaatt ttgatgtgct tatttgaatt
     4321 aaaattgaaa aggattcgac taaaataaag gaacaatgaa tagaagcgct atagagaatt
     4381 gtttcctgac ccatatttgc tatgatcgtg gactttaaaa caaataaggt taccaatgga
     4441 attagatgag cggcaattcc tagaaaatca aaaatcatga attcgctttg gagggatcca
     4501 taaatcaaaa ctccacctag aagtgacatg agtagatagg cttgcaaaag cccaatttcg
     4561 gcagcgtgat ttgagaataa aaaatggaat ggaccttcta gattagaatt cattgcattc
     4621 caggaaaaga aagtattccc aaatgctaga agtatcaata aaatcccatg aattcgaaga
     4681 atccacctag aaatagattt aagatttgac tgtttcataa cttatctcct tgtatactaa
     4741 gtatactatt attgtattat tagtatactg tcaagttttt tcttttataa aataatatca
     4801 tcaaaagact caaatagaaa ttagctcagt ttttaatcag gaacaggatt tatactaaga
     4861 aagaaaaatg aaatgaacga taggtataga gtgaaagaag aaatcataga aagagcctat
     4921 caaaaattat tatcttctgg tataacagaa acaacagcta attatttggc agatatctct
     4981 ggcgtaagta aaagaacttt ttataaatat tttccaagtg ttgatgagtt tttaaatcaa
     5041 ctcatggatt atattaaaaa tcaagtgcat aatgaattcg cttctgtatt gcaaagcagc
     5101 aataaaaatt atcttttatc cgtaaaaagt ttattcaaaa atttaccgaa agcctatggc
     5161 aaaaactttg aaaattttat gatacactta cacaaacaga gacctgactt aattaaatcc
     5221 tttcttgatt tcagaaaaaa tgaattcgat ttgattcttg aaaaactaat caatttacca
     5281 gaagtcaatc ttaaaaaaag aaaactcaat aaacgagtta ctagggatat tctaataaat
     5341 ctattcgaaa aaatggcaac tccatactac attgcagaaa ccggagaatc aatggacact
     5401 gtcgttcata caatctcaga aattttcttt catggaatca taacagaaaa cgaataaaag
     5461 acttatttct aatcaatcat tctattgtgg aattcgtatt tgaaatgtgc ttcctttcat
     5521 cagctcactc gtaacagaaa tagatccgcc atgtagatcg ataacttttt tagcaataaa
     5581 taagcccact ccaattcctt cataagagtc aacgttaacc gctctataaa acttatcaaa
     5641 gatgaaattt tgactttctt ctgagattcc gattccagta tcttttactg ttattctgat
     5701 ttcgtttttc aacttttcag agaaaatatc aagcttacca tcgcgtttat tgtaaaaaac
     5761 tgcattttta attatattca ttagcactct agtaaataaa gatcgatctg caaagacttc
     5821 ccctgctact gcaatattta cttctaattt tttgcgtttt atgatttcta tgacctttga
     5881 taaacataag tctacaattt gttttatatc caaattttcc ttttttaatt tgatttctgt
     5941 ctctagattt gtaattaaga gtaaatcatt tagaagatcc gttaacttct cactctctaa
     6001 ataaatggat tcaacatgag atcgaatctg ttctatattc ataggctcag tgccttgtaa
     6061 gagttcagaa aaaccatgta taaaggaaag tggggttttt aattcataag aaagattttt
     6121 gataaatgca tctttggtct gacttaatct aacatttaat tcctgtgccc gttctaagat
     6181 ttgattcttt tgattgattt cacgaagcat agatatgatt tttatattag aactagattg
     6241 ctgaaatgcg atagacactt gtaaactgaa tacttctaaa gtatttaaat ctgttgtatg
     6301 aaatggaact tcgtttgctg atttctctaa gtatataaca ccaattaccc gctcgtcttg
     6361 tttgaaggaa ttgcaaacat aatgaatcgt tttctcttca ttgtcatgtt taacaacata
     6421 gtctccctta tctttcccac cttctagatg cagactattt ttaaccaatg acttaatata
     6481 tttcaagata gtgatttgat tttcggtaat attttcaaaa aatacaggaa ctagatctcc
     6541 ttgctttttt aaaaaaatga ttcctagatc tgctgagaga gattgaatgg cagactttaa
     6601 aatctttgtc atcaatggtt ttacatcggt aaccactgaa agttctttgt ccatagaaag
     6661 aatattttga atcgttttac ttgcatcaat cagttcctgt cttgaaactt cttgcacaag
     6721 tctaaatgca tctacggttt cgtaggactg ttttctttct tccgcagaaa gatttagcat
     6781 cgcttcataa cctttaatcg cctgtcttgc atcagccaat tctctttggc ttaattcatg
     6841 caaagtctcg taggaggcaa taattccttt taagtcttca atctctcttt ttaattcttc
     6901 caatttttac ctcagataaa ataaaattca acaactaaca atcatttacc tattctaaat
     6961 aaatgcgatt gggtagcaaa attctagttt atattttgca tttttaatac gcttacctat
     7021 ttctttccaa cctaagcaag caaactctaa ctcaaaaagt tagtatttac cgagctcgat
     7081 tataatgcta ttttctgaaa tcaaatggtg ttgtaaagtt taaataaaaa tgacaacttt
     7141 accccttaat tggtaatacc gattcattcc actatcgtat ttcaatttat agttttatca
     7201 tcctattttt atcatttttt agggagaaat aaagatctaa agaatattta taattgactc
     7261 ataagactct aggcatattt taaaaatcct acaaacagga atcttgataa agaatttact
     7321 tttcttgatt atttatctca ttttccaccc aaaatatagg tagaggttac caaacttgga
     7381 actaaagaaa aaacatattc tgagactttt caagattgga attatttaca gccttctaac
     7441 cttttgcagt caatctgtgt atgaaaaccc atgtaacccc caaagcaaga ttctccaaaa
     7501 aaacctactt cttaaattct tattagatga taaaagccca tactgtgggc aagctagttc
     7561 aaacttcgga agaaatttag gaaacgcagc aaaagaacta actagttttt cttttctctc
     7621 acccccaata acgggtgata tcacagagaa gaaaataact ttaaccctac cctttggaac
     7681 taacgcaaca aatctagttg caaagtttac aacaacagga agtaaagtca gtgtaaatgg
     7741 agtgacgcag acaagtggaa tcaccgcaaa taattttaca tttcctgtga gttatatcgt
     7801 gactggaata gacggctcta cagagacata tacaataaca acaaccattc aatcaaatcc
     7861 aggaaaggca tattactcaa aacttaccac aggctctgaa agatatggga atgcggaaac
     7921 tctgaatggc tcaacaattc aatcctttac accagtgagc acatttatgc taacagcagt
     7981 aatcgactca gacagagata tcttatatgt gggctatgga gcggcaggag aaaatgtaat
     8041 cagggtatac gaaaatgcta gtgtcaatac agatacagta actagaacaa tcacagttac
     8101 acccaataca agaattggag gtcttgcaat tgactccata aacgatagac tctattctgt
     8161 taattttttt acaacagcta gttcaaatgt gaatcgattt atatttagaa tcaataatgc
     8221 tagctctcga aacggaacgt taactgctgt atctgatttt gcacagattt cctccaccgc
     8281 gctacatcca tctagtcaaa gtataagaag aattgcatta gactcaaaga gagatatact
     8341 atatatctgc gactatgggg ctaattcagt gtttgcgtat catggagtct caacttatat
     8401 acatgggtct ctttgggaac ctagtcgaat tataacttct gttccacaac cagctggaat
     8461 cacaattgat acaaaaaaca atattttatt tattagtagt ttcagtggaa atagcattca
     8521 tgtatttgat tcaggggata ctaccaatgg aacaactgga gcaaacagaa tcataacaag
     8581 cggatctttg aatggttcat ctggtcttca ttttgtctat ccgttcaata gactttatgt
     8641 tgctagctta aatgctggta gaattttata ttttaatgat gccagaaata taaatggaaa
     8701 tataaaccca gatggaatat caggagcgat ttcatcaccc acagatgttt cgattgatat
     8761 aaatagataa gaatttttta aaacagaaat ctatttagaa tagaatcgat ttctaagtaa
     8821 acaaattcta tctgaaataa aaaaatctat aattactcaa actcattcct ctcagagata
     8881 aaaaactttt aattaaatta ttcttgaaaa taaaatacac aaaaaatagt aataaccatc
     8941 tagagaagaa tatgaaaaaa aaatcagaat ggtttctatt gattgggctc attgttttag
     9001 tgatagtccc aacactcgcc ggcattgtaa gaattttcaa ccttggatca gctgaagcat
     9061 ttcaacccga aaacattcgg tttacgaaat cgcctatttc aatcacctta catgtagttt
     9121 cctccatttt atttagtata cttggtattt ttcaaatcat cccatccttt cgaattccaa
     9181 atttaaaatg gcatagaaga attggtagag tgcttcttcc tattgggttt atttctagtc
     9241 tcactggtct ctatatgact cagttctatc ctaaagtaga aactgatggt ccaacactct
     9301 attttataag actcttagtt ggttttgtaa tgactttgtt tttaattcta gcggtataca
     9361 cagtgttaaa taaaaagttt agagatcatg ggaactggat gattagagcc tatgcactcg
     9421 gactaggagc aagcacgcag gtgatcactc atctaccatg gttaattacc actggtcaat
     9481 tgcccacagg tctaacgaga gatatggcaa tgggagcggg ctggttgatt aatttagcaa
     9541 ttgtagaatg gagaattcga aaaacttagg aatgattttt attggtaaat cgttgttatc
     9601 ttcctgagcg gtttcttgtt atttaccact actcttagaa aattctattt tttaaaatga
     9661 caattcgaaa gatgtttcat aaaatcatca aattcaaatc gataaaagaa atcagctaat
     9721 gaagcttgag attattttac tcaatcgcaa ctcaagtgta ataaaataaa atatctattt
     9781 agtaataata cttgcatact agataacttt ttacatctta tctttaagga ttttaaactt
     9841 gaatattcaa agaaaatttg ccatctctat catgttactt ttttttattt ccatctcgta
     9901 tctaattcca gttgtttctg tatttcttgt ttatggaaag gagactggga tattaaacac
     9961 atcctatttt aatttactat taccttttac agtaactttt tcttggggga ttttattact
    10021 tttattctat acaaagtata aaaccaatat ttcaaaaata caatatttga ttttagcact
    10081 cctcatcaca gtctcttttt tatccttcac tggaagtcct gcttttagat atataaaata
    10141 cacagggatt tttgctttta ttatactcat ttttatttgg gttgaaacat tgattcagtc
    10201 tcagattata aaaaatcttt cgcggtatat aatgataagc ctctcctaca tcttagtctt
    10261 tctatctatt ctagaaccag ctctcatctt tccacaaatc ggtaaactgg agaatctaaa
    10321 tccatttttt aaaaaagaag gcttcataga ttttttgggt ttgtttgata atactcgaat
    10381 cttagaagga ggatatctca aacaaaacat tcattcaaat tatttaattg gggatggttc
    10441 actcggaaaa ataaatatta actcaaaagg gtttcgaagt aaatgggaac tagaaaatcc
    10501 aaaaccaaaa gatgagttta gaattttact agcaggagat agctttacag ttggatatag
    10561 aattgatgat gcctatacgt taggcggatt aattgaatcc gaattaaaca ataaaaatat
    10621 caaaacattt acagccggtc tattcgaaca atattccatt ttacaatggc aaaaacaata
    10681 tccaaaacaa tttgatgcag atgttttact cgttggaatt tgcttgggaa atgatttagt
    10741 gcaaacctac agcaaactca aatcaataga gttaggatat tctttcaaat atcaccccgt
    10801 caatgatccc tactacaatc aagttttgga aaaagaaaaa ctaccattaa cagctttaga
    10861 tagtaaatcg agttttaatt acaatgtttg gaatcctgca acatcaattc gaaaagatct
    10921 tcgtattgtg gaattggtga aactatacag accaacagga atttggacta tgcctttgcc
    10981 tggttctatt cctacttggg atccaatgaa tggattcggt gcctttctaa aaaattcaga
    11041 gaagacctat tctgaacttt ttcaatactt tggaaataca ctttttgaaa ttcataaaaa
    11101 cgcgagtaat gcaggaatta aagtccactt tgttatatac ccaatgcgat ttcaacaatc
    11161 agaagcagaa tggaaagcta caaaagaatt atatggctta gattcatctg gttttaattt
    11221 ggaaaatcca aatcaaatca tcgcaaatat ttgcaatcag tatcaaatgt cttgtttaga
    11281 tttattgcca ttatttaaag aaaatggtaa agagattctt catttcccat ttgatcaaca
    11341 ttggaataaa aaaggaaatc taatcgcagg aaaatacata gctaatttca taaaagagca
    11401 acatttcaga aagtaaatga gtggctaaca accaatagcc actgtatttt tcgaaaacaa
    11461 ttaagattaa agtatatttc gtaacttctc atgcaaaact tggaatcatt ttggagctta
    11521 aatcgaaagt gaatggagag cggttttttt ttataaactc atctattcaa tcaacttaga
    11581 tcgataggcg aaattctcta tttgattttt taaaaaagat tctttcattc aaacttatta
    11641 gagataatag gcacagttca cccatatttt ttaatcagtg agacaatcat tgacctatgt
    11701 taaaataact tgttttttta aatatgggtt tacctttgaa atcaattcct cggctcgaat
    11761 tggctttgta ataaactctt taatatccaa ctcaatctgt ttgttttcaa aatcatttga
    11821 ttcagataaa tcagcactca taacaaaaat aggaacatct ttccattttg attcagacct
    11881 tagttcaaaa ataaattccc atccattttt ttcaggcata ttaatatcgg tgatgattat
    11941 atctggtttt acattttcta aaatttttaa agcctgattt ccatttcctg cctctaatat
    12001 atcaacccaa atatctgatt tcaaaaaaac atcctttgca attattctat gagcttctag
    12061 gtcatctaca atcagaatac tcggacgtat ctttggaagt ataattttaa agcatgttcc
    12121 tttgttttta tcagaactaa cgtctataga accgccgtga gcatcaataa tatctttaca
    12181 atagggaagt ccaagtcctg ttcccttttc tccatgcaaa cctacaagtg tagttttaac
    12241 ttcatgttta aaaatatcag gcagaatagc aggcggaata cctgttcctg aatcttttat
    12301 ccataaatag gtataatcct catcttcctt ttccattccg acaaatatag ttccaccctt
    12361 cggagtaaat ttaattgcgt ttgtaagtaa atttgagact acctctccga gtaaatgtgg
    12421 atctgcatat atacttttgc ccgattctat ttcattctca aagtgaatac ccatttcttc
    12481 cattttaaca tttaaagtca tatgtatatt ttctactaac gtctttgcat ctaaatgttt
    12541 tttaattgga atgattcttc ctgtttttaa ccgagtaata ttgagtaatt gatttaataa
    12601 ttcaatcaaa ccagttgaag ttttatttat catggttaaa aactttatac ttccttcctt
    12661 ctgaattgta tctttgtatt ctggagaaat cagcatctct gacattgttt gaatcccacc
    12721 aatcggaccg cgcaagtcat gtgatactaa agagatgaat ctatctttta gttgatttga
    12781 ctcttccgcc tgttttctag ccgaacttaa gatttcttct actctctttt gttctgtgat
    12841 gtcttggata ccagcaagaa ttgccatttc tcctctaaaa ttaaacatag catatgaaat
    12901 aattgtccaa aactctctac cttcaaaatc tcttaaaagc aattcggcag taacaacttt
    12961 ttccggattt tgtcttagct tttgaatata agcatcgcga cttgggggat ccactacaaa
    13021 gtttggaact cgaacctgac ttactttctc tcttggaact ttaaacatat taattgcttt
    13081 ctcattcaca taaagataat gattgcctgt agaatgcgtt attacgacag gcataggaat
    13141 tgcttctaat atagacgata attcctgttc tctctttttt agttcttccc ttgcaattac
    13201 attttcagta atgtcaaccc caacagataa aatagtttta ttaaatgaaa tactatttcg
    13261 ccaacgtata gtcacaatgg atccgtcttt taacttattt tcgttttcat tgtcagcaaa
    13321 actctctgga ttttttaaga aagagttgat catatcttgc atatctctac cggcggagtc
    13381 tttttctggt aaaattgtat ctaaaatatt ttttccgata atttcatttt cttcatatcc
    13441 gaaaaaggat agagcgtatg cattcataaa taaaattttt cctgatgaat cccaacgaat
    13501 tataattcca tttgtatttt gaactaaatt tctatattcg cactccgatt caaaaagagc
    13561 tttctgagct atttctgttt cttctttttc tcgtgttata tttacagcaa gtctatagag
    13621 gacatcgtgg tttttcccaa tcttataagc tgcaaatatt acagaaataa aaaagagaag
    13681 ataaacctcc ggataataaa ttcgagaggt ataagaagga tatagaaata aggttagaat
    13741 gacaggaaga aaggcgggta tagcaaataa tagtgtatat cgaaagctaa cggttgttac
    13801 gcaccagccg gaaattacaa aaagtataat caaggacaat agaatcagat tggttttgtc
    13861 aaaaccattt gcccctaata aaaaagaaat tccccaataa aatcccgcaa gaaaggtaga
    13921 aattttttgt aggttagtta caaaccgatt tttatttccc aaaataaaaa aaagaaggaa
    13981 tcgaatgaaa agaagagcaa aatgaagtgt tgcccaattg ataaccaata gagaaccttc
    14041 tagagaggga agaagaaatt ctaaaaaatc aatcgattca gagtggccaa actgggatgg
    14101 attttctttc acaattaaat aaattaaaaa gcctgccact ccctccaaga agaccatgaa
    14161 tcgaccttgt ttaaaaatag aagggatttc ctcctccttg attccacctt ttaacaaaaa
    14221 ttgttggtat gtttgagaat ccattttaaa aatccttttt attttgaaat taccttgtgt
    14281 aaatcgattg gattgatccg cttataaaat acgcactctc attttcacaa cttagaaaaa
    14341 atacagactt taactgaatc aatcgaatgg aattggattt ccaaattcca ttgtatcctt
    14401 tcaatcctgc tcaaggattt ttccattcgg aaaagccaag ataacaattt atcttctgat
    14461 tcttctcaat tctaataatt tatgtattgt taattgcgaa ttttattcta aaaattctat
    14521 gatcgagttc gatgcggtaa ctcgattaaa cactatttat agtaaaagat ttacaggcaa
    14581 ctaaatttaa gatatagaaa agattcgtag atttttttct ttgagaaaat ataaaaattc
    14641 catatcaata agatgaaata gaaaaaatct tctagaatta gaaatcatct tatttctttt
    14701 tttgagtaga accaacactc tgcttcgagt tagactcaat aaaaagagtc tcatggattt
    14761 gtgaccaaat ctaaagggat accgctcacc cgagcaacat gttgaaaaaa gttcgaaagt
    14821 tcatcgaagg attcctcgga gaagactatc aaatgggaat ctcccttttt atcttctact
    14881 ttccattcca ctaaaatatg agcttttcgg attttagcaa tcccagaacc atgccgtgta
    14941 acatgacgca tttttctaag ttgcatctta gcatcatcaa tgaggcgaaa agttttctta
    15001 ccacaaaata attttcctgc ctctgcttga atacgaaaac tagatcgaag agaaagtaga
    15061 atcggccaaa aataaattcc taaaaaagcg agcggtgtaa cgatcgtaac cagaagtctg
    15121 aataagaatt tttcaggagc atctctataa gcatccacta agttccaatg aaaaatatca
    15181 gagagaccat ttaaaaactc tgtcgtaaca acaaatcccc ccgcaacaaa tacaaacaaa
    15241 agaattgcga aaagaaaata gtataaaatc acttctttca attcctttga gggcaatttc
    15301 caaattcgat tgtcactcga gagacttgag tgtttaaaat caatattcca aatggcaaaa
    15361 ctagatacta tcatgattta gaattccttt cctgtatttt ttcaaactca tgcaaacacc
    15421 aataagtaaa tactatccca attggaaata cgaaaacata aaagtaagtg cagactttta
    15481 aaattttcag agcaggtttc caacgaagca aatgtagaat tcctccaagg atggatggga
    15541 ttccgggaag aaggattaaa agaacactca atacatttaa taaaattaca ctcaaaataa
    15601 gtaggaatag ataaattaaa aacacgaata aattccaagg gatagtaatc aagagagcaa
    15661 agaaccactc gtaaatactt gattcagtcg aagggagtga gaaaagaaaa aaatttaaaa
    15721 gtgcatttaa ccatttccca tccaaataga actctttgga atattgaaaa tctagacaga
    15781 ctccaatcac aaggatacca aaaagaatgt gagcaattgc aattttagtg gagatttgac
    15841 tcatagatta tctttgtcct tagcattaca aaaatttatt atgatcatat aaggcaggaa
    15901 gctttttgag caataattta aaactccgag ttgatatcca cttatgccat aattgaaaac
    15961 acctagactg aaagcagatt ctccctcctg tatatttagt aagccaatat tgaaactatc
    16021 gcttatatta acgattccga gtgataaacc gaaattagag tctctctgta atcctgaatt
    16081 aatatgatcc acatcagatt tttttttatc accgagagga acttgattat tttctagtcg
    16141 tttatcaaat ggaaatagaa tattaacaat tccaagtttc agaatttttt tattagtatc
    16201 actaaaattt aatattccca attgcatagc aacacctctt tttaaatcat ttaaaaaacc
    16261 aaactgaaga ccgaatattg tattgacttt attattaaac ccaatattaa ctccgaataa
    16321 attttcaacc tctcctgaaa ataaattaaa tctaaatact tcgaaattca tattttgggg
    16381 aatagtaatg ttctcttttt gagtaatgga aactccacat ttgtagaaaa ggaagaagac
    16441 taaaataaat agaaacttaa atctataata atttctatta caatcctctt tagaattaaa
    16501 caaactcatc tacttcccgc ttcttttttg atttaactat aattatattt tgaatgatgc
    16561 aagacttttt aagtttatag attgagaaat aacattgatt tacctcgatc gaattagatt
    16621 tctcttttct attttaaaat aaaattgctt gcttataaat taaattcaaa tacaattccg
    16681 ttttaaggaa attagaatgg atattttagg ttttcaaatt agagaaagac ttggggaaca
    16741 attacctggt ggtcgtttaa atagcacaat cttctttgat gaaaataaaa ataaatatat
    16801 aagtagaaga cctgttcgct atatagattt tacagtgaat ggaaactcta ttgcttcact
    16861 tattgatgct gacctaaatg acattgttgg atcaatgcaa ctgaaacaag gagaagaaca
    16921 attctttgga atttcgactc acgttcttga aattctacaa ttcaagtttc catttacttt
    16981 aaaatacaac agagttcctt tatttgtttg tcctgaatgc ggatatgatg aaaattgtgg
    17041 aagtgttact ttcgaattag cagaaacaga agatcaagtt atctggcgtc actttggatc
    17101 tggatttgaa acagaggcaa cttcgtttat gaaagaattt acttggtctg ccttagagga
    17161 agaagacttt gaattctgga tagaatgtgg gaaatcaaat gctattctag aaaaatttaa
    17221 agggagtcgt tatgaatgga ttcttggagg aaaaccttcg acttgcgaaa tagactctca
    17281 aattgattta actaatttag aatatcccga aattggacca tttacattta agaaaaaaga
    17341 ttattttaat gtattatccg aaatagaatc aactttaaaa aaatatcgat tgtccgaaaa
    17401 ctaaaaccat tacaagaaaa tcttctcttc tattcaacac agggtggggc aaatccaatt
    17461 tctccgtgat gacgaaccat taccaaagca aatggaatag aaattggtaa caacacagta
    17521 tctaataaaa atgaaaaagg aagatcaatt aaataaattg gatcaaattt tccactatcg
    17581 ttttctatca aaagatttac atcacacctt gttcccgagt agaatactgc tgtatttttt
    17641 ttataccaag atggtataga caaaagagtt gcgcattggt ttagaaataa gattaaaatt
    17701 aaaatatatt ttttcatacg acaggatttc ctttttgaat cagaccacat ttcattcctt
    17761 ttaaaaggat catcttccta gctattgaaa gttaatctag aaatattttt atttccttat
    17821 ataaatctga tttttctgat ttctctcaaa tatccatttt gttttaatta gagaatctaa
    17881 aaacttttca tcatgactta ctaaaacaat acagccatca tattcggata acgcatcttc
    17941 caaacaatga acagatggta aatctaaatg atttgtcggc tcatctaaaa ttaaaactgc
    18001 aggcttttgg atcgcgccaa gtccaaataa aacctttctc ttttctccgg gactaggatc
    18061 tttagaatag aatacttgtt ctggaataga acccaactga tagataactt gataaatctt
    18121 tccaagctct tccttgtttt tctgtcgaat ttcattttga attttttccc aataaagaga
    18181 gtcaatttct tggggtaaat aaaacaccaa agaactagga atgtttaatc tttcataaag
    18241 atactttaga aaactgcttt tacctgttcc attttcccct tggattccaa ttttagaatt
    18301 aggctttagg tataactcag gtataaagat agaaatttca gaattaggtt tccaatcctg
    18361 ttcagtaaaa tgaaaaagaa tatctctata aatcggagta gattgaaatt gaaaaccttc
    18421 cattctatga acctttttat atagaacgtt agtttccttt tctagctcta cttttttatt
    18481 ttcaagcaca acttttttcc tactcccttt tttatccttt ccggaaagtc tagctcgatc
    18541 tatttttcct ttcgtatcat gatccaatcg atcaatgttt ttcttagaaa gactattgga
    18601 atgtgaaaat tctttttctt ttaagctttg aatgcttctc ttggctctct ctaaattatt
    18661 ctttgcaatc ttatacttca cttctctcga tttttgagat tctctttctt gtgttaaact
    18721 ctccgaataa tttccggaat aaatagaaac ttctcccttc ctaagaaaaa tacatttatt
    18781 gcagagagcg tctaacaaat ctcgatcatg gctaataaga ataccaatcc ctttaaaagt
    18841 ttctagggct tctaaaagaa ttctcttccc agaaatgtct aaatgattgc ttggttcatc
    18901 caagatccaa atatcaggat ttatccaaag tgaaacagca atttgaattc tctttctctc
    18961 tccaaaactt aactcctccc aagagtaaat ccaatgatct tttattttta gtaaggaacg
    19021 atagtagcca gagaatctat cttcccaaaa ttcatctaaa ccatctatac gagaaaatga
    19081 attctcagtc attaagattc gattctcttc taatggatat ttaatctgac ccgaatttgg
    19141 ttttaatttt ccagaaatga ttttcgcaag tgtagatttt cctgttccat tctgtccaac
    19201 aatcccagtc catccatttg taaaatgaag attgagtgag ttgaataaat tttcctgcgc
    19261 tggagtatat gaaaaattaa tattttgtag gcttaggttt gcaagcatgt ctctctccct
    19321 gttaaaagat tttttgtgat ttgagagaga tatttgtcta tctctcttaa gagagtgaat
    19381 tcatcggctt ggattcctta ttttttatag ataagatcta tacacttaaa tatttttaat
    19441 attcatccgg tcaagttttt ttattctctt tcttctaaat gccttacttt tttcgagatg
    19501 gagctattct gtattttccc ccgaaatctt tagaaagttc tttcccgagt ctaacttact
    19561 ttttagatat tgagtatcta ggcaaacatt gtatgcatta tttaaaaatg aattctttcc
    19621 tctaatcaat aaaataagca ggcattttta cctagataat ttgaatagat ttgaaaataa
    19681 aattttcagt tgaagaatga ttcgaattca tttttactat ctaaatcttt tttccttttt
    19741 cgggatttta aatagactaa taatagtatg aagtttttaa aaaatttatt tttacctaaa
    19801 atccaaaaag caattctaga aaacgatttt aataaaattg agaattcaga aatcaatgag
    19861 tatttttcga atggaagttt ccctctcgaa tttgcaatcg agcaagacaa atgggaatca
    19921 gcaaagtatc tacttgataa caaagcagat ccagataaga gaattcatat agatgagcaa
    19981 acgttagtta tgagtatcat tcaaaataat tataatttaa aatatctaaa tctactcata
    20041 gaatacgaag cgaatttgaa tttgaaagat aaaaatggat attcagcagt catccattgt
    20101 atcttaactg gacatttaga agccttaaaa attctcttag agaaccaggc agatccaaac
    20161 ctacaagaca aagatggagc cactgctcta aatcacgcca aatccactgg tggactttct
    20221 gaactcatcg atttgcttct tgaatatggt gccgatccaa atataaaaga tagacatggc
    20281 aaaacgtata atatgtaatg ggaaagatta gtttgaatac tctctttaaa agctaattta
    20341 gtaaatgtag aaacttttac gttagtagaa atagaaaaaa atatttatac ttatagattt
    20401 cttttaattt gataaggcat gcctgcttag actatataat ttatgttcta ctttcatccc
    20461 ctatccattt taaaagaatt ctttgtagat cttctataaa atatggtttg gttaaaaagt
    20521 cattcattcc tgctcccaaa caaatttgct gatcttcttt cattgcattg gcagtgagag
    20581 caatgataat aggtgattgc aaagaaaatt tttttcgaat ttcttttgtt gtggtgattc
    20641 catccatcaa gggcatttga acatccataa aaattaaatc atagagagtc gaatctagtt
    20701 cctccaagac cttgtatccg ttgtcaacca tctttacttt aagatttatt ttaagtaaaa
    20761 gcttagataa aactcgttga ttaataggat catcttccgc caccaatatt ttaagatcat
    20821 ccctccattt aattttctca aaattctgaa cacttaacgt cttagcaata gcaaatcctt
    20881 tcttcgctat aatttggaaa gaaaactcac ttccttctcc tactttactt ttcactttaa
    20941 gttcgccgcc catcaagtga attaactttt ttgatataga taaacctaaa cctgttcctc
    21001 catattttct tgttgtgctt gcgtcagctt gtgaaaatga ttcaaatatt ttctctattt
    21061 tatcctcaga aattccaatt cctgtatctc taactagaaa cgataagaga aatggatccc
    21121 cctcctctaa tactttcgct tctaaaaata tcttacctct atctgtaaac ttatttgcat
    21181 tgctcattaa atttaatagt atttgtctaa ttcttacctg atctcctaaa atatattcag
    21241 gaaaggaatc gcttataatc aattcgattt cattgccctt ttcctttgct tgtattcgca
    21301 tagaatctac aatttcgact agtaattttt taacatcata ggagatctct tctagttcta
    21361 ttttacctgc ttctatttta gaggtatcta atatatcatt tatgattgct aacaaattat
    21421 ttgcgctcgt tttaatcata gaaataagtt tcatctgttc ttcatctaaa gaagatgact
    21481 ctatgatttg aatagttcct aagataccat tcataggagt ccttatttca tgactcatat
    21541 tagctaaaaa taaagattta gttttatttg cagaatcagc taattgcttt gcctctctta
    21601 actcttttac taatatttct aattcttcat tcatctctaa aattttttct acattcatag
    21661 aatgaaactt ttctaaagaa gaaaccattc tgttgaaagc atctgcaagt tctacatact
    21721 catcataata ataaatacta tttgtatcca aacgttcgga ataatttccg gaagtaattc
    21781 gttttattct aactaaaaac ttagaaattc tttttttaat taagtgagaa aatccaaggg
    21841 acagtaaaat agtaaaagga atcgaaattg aaaaatatat gatagcaaaa tctgtaactt
    21901 tatttatgtc cgaaaaaact ttgctctggg ctttaccata aacaagataa agatctaatt
    21961 caggtatgat tcgaaagaca tacaaagacg ttatgtcatt ctcttttaga taaaaagcac
    22021 cttgagtcaa tttagctttt aattgagaaa gtgtttcttt ttccagaaga atcgattttc
    22081 caaacggcac attaccagtt atatttaata atacaattcc ctttgcattt atcagataaa
    22141 tctcttctga aaactttaaa ctgatttcaa tcaatagtgt gtgaacaata tcaatagaga
    22201 ttaaaaattt tctccccgaa ctttcattgt atattaattt tatatcattc tctttttcat
    22261 aaaaaacttg ttctggagta agatcttttc gtttagataa tggaacttct tctatatagg
    22321 atgggtcaca aagattattc ttaatacaat cgttaatctt tattttataa tcatttagaa
    22381 caggatttat acctgtcaaa atagcatcgg attgttttaa ataattatta acgatttcat
    22441 cttctatcaa tttttgagta taggatccat aaataaacaa tgtaactatt atagaggtaa
    22501 gagtcgttat taaggtaaat aaaaagacct gccaagaaat aggaattttt ataagttttt
    22561 tatttatttt cattttatat aattaatatt ttggatgtga atatttgtat taattttttc
    22621 tttttctaaa actataattt tatctaacgt tatttctcga gcatttccat accaatcagc
    22681 agctttaatt tttatgataa attctcctgg gttgtttaaa gtcagaggat taatacaatc
    22741 tctatattct gaattattga ttgagcaatg tatgtataat aatccaatgc ctgaatcttt
    22801 cgctgcaatc tttatttcag agcctacatt tgtatagagt ctattgatca aattgggcat
    22861 tatcacttgt atttcaggag gagcagaatc atgaataaag ctaatcgatt tttttgaaat
    22921 attgcctttt ttatcttctg ctgtgaccac aagaaaaact ctcttatcat ttaaatgtgc
    22981 aggaatataa attatttctt ctctatttaa tgggataatt ttatcttcat attcaaattg
    23041 tgcattccat gattttactc cgcttattaa atctacaata tttactgtaa taactgtatt
    23101 cggtccagtt aagtatacat tatcctcttt tacaaagtag tgttttattc ctaaatgaat
    23161 ttcaggttcg gaactatcga ctaaatatgt tttcttttgt attaactctt ttttacctag
    23221 cttatttata gaataataat atacttcgtt aagaccgtcc ttatccaaat aaaatggagt
    23281 catgtatctt ctccagggag aagaattcaa ttggtagaaa atttccgatt ccctgtcact
    23341 tgattccaag ctaataatat cactcagatt ataataaact ggctccacct ctggaatcgg
    23401 gaccggctca gaaatcgcaa taggtttcac tgtttcaggt aacgctttgg gaataacaat
    23461 tcgtggttga atttcaaaag tctttgaaaa atttccagtg gctcctattt tatcgattcc
    23521 agcaactcgc ccatatttat atttatcatt ataattaatt cttaggcttg tttcatttat
    23581 cctagttgaa tagacaatag tgctaaaatt ttttgtattt gaaatctcaa ctaaatactg
    23641 tgatgctccc tcattcggtt cccattcaag cagaatgaat gaatcttctg caaagctctt
    23701 acctataaaa aaaagaatta aaaaaagagc aatcgttaga aatatattat acttaacaaa
    23761 cttaataaga atcactatta ctcctttaca ggtttcggaa taatttcatc tagtagttta
    23821 aaaggctcaa gaggtggttg tccttttaca actttagttc cgtatccagc cggcacttta
    23881 accgttatgt tctgagcgtt aacgtccact aatcctttat aacaaccaag tagtgtagtt
    23941 tcctgtttat caaccgaaac ataaaattct gttcctctca cgctcgcaac agaggaaggt
    24001 gttttgactc tgtatttaat tttatctgtt tcattttgat tcaatacttt taagaatata
    24061 tttcctagtc ttaaatctaa acttctttcg ctcgccttct ccagcttaat gattgatttt
    24121 tccctaacct gaatgacggt taacggctgc tctaaatatt caatttcagc gctagatttt
    24181 tctttagttc taacaacatc gttttcaaga aatttctgat ttttttctac atttttccaa
    24241 tctagttcat tctgtttata ttgaacttcg cccagaaaaa ccttcaatcc agcgatcggt
    24301 tcaatttttg gttttttact taaatacgct ggtattttta atacaacccc aggacgaatc
    24361 atatttggat tggcaatttg attgtattgt aaaagctctt tccatttatt catatcactc
    24421 agtttttcct ttgcaatctt agaaagagta tctttatctt ttatcacata ctcttctata
    24481 tcctctgaaa aaatagaaat ggaaattaaa aacactgtgc tcaataaaaa tctattcata
    24541 taatatccta agcttataat acaatctgat acagaattag aaaaacaagt ataaaaaaga
    24601 atcatggctc aatggatgca attgattttt agttatcagt atttttagag cacttagtct
    24661 ttaaaattat acttcgattc gaatgaaact gtctgcgaat gaaccaaaca gtctagatac
    24721 tagtaaattc ccctttcttg ttcttatatt atacggattt tctagacaaa atgtatcgaa
    24781 tcgtccaatg actgttttga tcttcagtgt ttaaaaggaa atcttacaaa aaataaaaca
    24841 gaaaaaaagt ttcctcatct tactttttat tactagttct tatatctttc acctctacca
    24901 actcaatcct agtttcattc cttttcgcga aattttactt taagtataaa accttagctc
    24961 gcattccaaa tactgaaatc cctcttctca ttgcaatttc caagaatcca aaatcgatcg
    25021 atcaaaagca gaagaaggcc aaaagaagaa agtaaggggt aacatagata accctctgaa
    25081 ttgatctaat cgaaggattt gctttgtctt tgcgatttct tttttcccct caaagtaacg
    25141 ataagtgata gagtgctcag taattgtgta gaaaggaata ataccaagag taaaaaggct
    25201 tgcgaccata tttgtaaaat gcaaaaatcg atgcgagtat gtatgctctc tctcttccaa
    25261 aatcacttct aagagtaaag atgaattcga atcttccata aaaccagatt caagaatttc
    25321 ctttttcaac agtttctttt ctttctcata aaaataaaat cctgtaaatt caaggcgtat
    25381 gcttttagaa gttaagtttg gattttgttt ttcatacatt tgtggttgat tgtagaaacc
    25441 agcacaaccg atgaaatgaa tcatcagaac aagatagaaa aaactctttt tacaaaactt
    25501 ctttatatgg caggaaaaaa gtttattcat agagagtctc cgagtcattt aagaatttca
    25561 aagtataatc ggaataaatc gattctattt cttttttatc gtcgtagaac attaaaataa
    25621 atgaaatcca tccgatcgcg cgaattgttt ccactggata aatatattct cccttcttca
    25681 gattgtcttt ccatatacga aaggtaattc tttcttctct tacttgaaaa atgggaagaa
    25741 caagacgggt tgccgcagat aaaacacgat ttagtagata aagcgtatat cgtcctggtc
    25801 ttttctctgc tacccaagaa acaggttgca caggttctcc taaaagaatt gcaagttggg
    25861 gggattcatc taaaactatc tgaatcttcc actctgaatt ggtagaattc gcataactaa
    25921 gacttttaaa ctttttagat ttttcaaatg tatctaagat actcgtggct ttgattcgat
    25981 tttcttttcc cttccagcca attagttcat acgagatatc cttttcaaat ttagaaacct
    26041 tcggatttac agttggtctc tctcctaata ggaaagtaga gcaatgaata aaaaataaaa
    26101 atggaattag aaataaaatc gaatttaaca ttttgcaaaa ccaaaaatag agtatgaatt
    26161 ggaagcaaat cttgtatagc ataaaattct cttttttaaa ttcccagtct agaaatagag
    26221 aaagtagctt atagtttgta atcccgaatt gtgaaattac gttatgtaaa tagattgagg
    26281 attataaaaa cgattcaacg aaaaagaaga tagagaattt tgtatttttc ttggcaaata
    26341 aaaccaaaat gcaaaagtag tgcatctcat tctgaagtca atttctatgt tcaagtttat
    26401 tttaattctt cttttctcaa ttcagctttc taattcaatt tttgcgttag aaccaatcct
    26461 attagagaaa aataaagaag aatatagtat tggaaagtat ctagaaattt tagaagacca
    26521 aaccagaacc aaaacttttg aagagatcca aaaaattgac acaaaagtat ggcgaaaatc
    26581 agaaacagac actcccaaca ttggtcatac gatatcagct tattggttta gggctaatat
    26641 agattatccg cttgacaagg aaatggaata tttcttagaa attgcttatc ctgttttgga
    26701 taaaattgat ctctattttt tagaagaagg gaatcagaat tattcccatc taattttggg
    26761 cgataaattc ctttataaag aaagaatcgt tagcttttac caattcgtat ttcctttaaa
    26821 attaaatcca aaatcaaaat atcagattta tatacgaatt gaaacacaag gaaatctatt
    26881 agttccaatt acaatttata acttaaattc ttttctaaaa agagtcaata gttcaatgat
    26941 tgcctatgga atttactttg gctgtattct tgtaatgatc ttttataatt tctttttata
    27001 tctttctatt cgagacagaa gttatcttca atatgtattt tacctgatga gtttttttat
    27061 attacaagca ggtgttacag gtattgggct tgcccatata tggtcaaact accctatatt
    27121 caatgatgga atttctatct actcaagcct tctcggaatt acaattttag gctttactag
    27181 ttcctttctt agaacaaaag acttttttcc tagaatgcac aggctaacaa agattagcca
    27241 atggattttc ggaatttcca ttctccttta ttttattcta ggattttcca ttacaattag
    27301 actcgttctt ttattaggat taattacaat tctaattgcc tattcaattg gtatatccgt
    27361 ttggcgaaaa ggttttaaac ctgcaaaata ctatttcttt gcttggagtt tttttctttt
    27421 tggaggcact ctttatattt taaaaacttt gagtctactt cccagtaatt ttctaactga
    27481 atatggagct caaattggtt ctatatttga atttatttta ctttctctag gtcttgccaa
    27541 tcgcatcaat ttagaaaaag aagctaggca aaaagcagaa aaactggctc tcgaaaatca
    27601 acacagagca gaaaaattta aagaagaact agaagcaaga aaaagagcaa tcgaattaga
    27661 agagtctaga gatcgcctat cgattattgg acaatcaaca gcatcgattg ttcatgattt
    27721 aaaaaatcca attgcaacaa ttagagccta cacagaactt tctatgggag atgaaatctc
    27781 ggaaacagac agaaaggaat atttagaaat ggttctgaga gaaatcgaaa ggcttggtga
    27841 tatggctttt gaaattctag actttaccaa aacaaaaatc aatctatctt taacggaaac
    27901 aaatctaaac gatttcttag aagacattta taaatttctt aaactcgatt ttgactatgc
    27961 tggaattgat tttaaagtga aggcagaaga tactatcatt ctaaatatag atagagaaag
    28021 aattcgaaga gttctgatca acctcgcaaa caatgcaaga gaagcaatgg cagaaagaac
    28081 aaattctcaa ttctccattc aatgttttaa agatggaaat gaaatcgttt tatctgtagc
    28141 ggataacggt aacggattac ctgaccgagt gatagaaaat atcttccaag cctttgtaac
    28201 agaaggaaag aaaaatggaa ccggcttagg actctatatg gcaaaaataa tcctagaagc
    28261 ccatggagga aaaattacat acgaaacagg caaatcaatc ggaaccactt tctatttgcg
    28321 atttcctttt tgctgaagta aaaggaatgt aataaaaatc acttttttat ttgttcagat
    28381 agaaaccaat ttccaactac gccagttttt cattgcaacg attgatatag ataaatgcga
    28441 tcgagtcttc tggacatttt ttttcgcact cgataaaata ttcttttgct ttctgaaatt
    28501 gattttctct aaatgcaatg agtccttttg agaaattttc tagattcttt tttttacaaa
    28561 ttcgaacctg ttcctcatct gcatcaaagc attcatagat gactatcggt tttgattttc
    28621 ctcgcacaaa tacagtatcg atttctctaa ttgaaaattg ttccttgttt tgcagatgat
    28681 tataaacagt ttccgaaatt agaatcggaa tgtagagagt ttttgtaagt gactctaaac
    28741 gggatgcaag attcactgaa tcactaatca cagttccatc cattctattt tttccaccga
    28801 tagttcctag cattaagttt ccagtattta aaccaattcc aattttaatt ggacttagat
    28861 tctttcgaat tctttcttca ttatagattc tcaattctct tagcatggag attgacgcat
    28921 ttaaagcatg ctctgcactc tcaggaaata aagccatgat agaatcacca atgtatttat
    28981 ctataaaacc attgtgattt gaaataattg gttccatcac tttcaaataa gaattgataa
    29041 aattaaaatt ctcctgagga gtcatagatt cagaaagaga agtaaaacct ctaatatcag
    29101 aaaacattac agtcatttct ctctgaatct gatctcctag tttcacatct atgatactag
    29161 ttttatttaa tacacttaaa aattcagtgg gaacaaaacg actataggct tcatttgttt
    29221 tttgtaaatt ttcagaaagc aattcaactg atagaaaagc tttagagaaa cgaagagaaa
    29281 ttaaaaagga ttgagcaaag ataaagagaa ataatccata aggcaaaagg taggcggtat
    29341 tgatgacagt atttgcgtat agaatatcat tgatcactgt gctaattaaa aataaaaatc
    29401 caacgagaga tgcaagagct ccatctcgat tgtggattac gctgagcact aaacaataca
    29461 gaccataaat acaaactaga atgataaata tttcccaagg aagcgcagtg taagagtaaa
    29521 ctgaaatatc tgtaacaaat agtaaagcca ctaagaccaa tgagatagaa ataaaaatct
    29581 ttttaaataa attcgaaaat tcatttggaa aaatagacat gataaaagct gcaaaagttg
    29641 gaatcactag atataaagtg ccgtattcaa ttttattccc aagctcccaa ttaaaatttg
    29701 gaaatttata aaagagaagt ctctctccag taaccaaaag tcttagcatg acaacaaaac
    29761 aaaataaagc aaaatgaata cttgaaatat cttttcttct aagaatgtaa agacctatgt
    29821 gatacactaa catgatagaa atactgccac ttaaaaatac atccatccaa actttgtttt
    29881 ctctgtggat ttttaaatcc tctgaatcgc ctatcgttac actctcccaa aatccacctt
    29941 tataatggtg aaaattacta atctcaacaa tgatttcatt ttcttgtttt agattcgtca
    30001 gctcttttgt tataggtaaa aatctaggtt cagttgactc cttatccgtc cctgctcttc
    30061 cattggataa tatcaatttg ccgttcaccc aaagattata actagttgcc gcatctaaaa
    30121 atcgaatgga gattggagta tctgtttctt ttaaatataa gagaattcga taagaagcat
    30181 aaccttgtcc tgagatttct ttcccatcaa tgacaagccc attccaaatt ccgggaacct
    30241 gggcaaaata tttattttct cgacttacac aggattgtgg attcgatgca agttgacaat
    30301 tccagtaaaa ttcccaatct ccatctagat tgacctttcc aagagaagtc aaggaacgta
    30361 gatctaaaac accttttttc acggaaggcg gatttgaatt ggaagtttga ttgcaaaagg
    30421 acaaaagaat cagaaagaga acaattatat tttttttcat ttgatttaac tcatttttaa
    30481 taggataaga gacgtatgcc tctaatcctg ataggttttt tacaagagaa gtatataaat
    30541 tacgaagctt agtttttttt tcaactagta aatcaaaaac accaaacctc tcagaattca
    30601 accaaatggg aaaggtagaa tttctaacaa aaagaaacga ttttaaaaac taattcccca
    30661 aatccaaatg tcttttttta aaaagagtaa tcccaattaa aaaataaaat agagaaatga
    30721 gggcgagtaa aatcacagga gttagtattt cagaaatact taagccatca ttgaaaatcc
    30781 gatttaagga agtaacagct agagtagcag gaagaaaatc attccagcaa actccatgat
    30841 ctaacacata aaaaattgga tttctaggaa ttggaatcat tgcacctgta aaaaatatta
    30901 aaataaaaag tgggaattga cctacgataa caaagacact tgaatttctg caaaaactca
    30961 caatgataaa agcaattcca atgattccaa taaaggtaat acaaaataca attagaatag
    31021 aactaaaaga accaaccatt ctaaaaccta aaacctctgc aagtcccaaa gtaagtaaga
    31081 ctgataccgc tccgactaaa acttgcgtta gtgtaatcgc aattgcataa tcataaatat
    31141 tcattttggt aagttttaat cgattcatag tgtgatcttc tatgtctcga acgataatca
    31201 tcccagctga aaatagaagc ataataatag agagaataaa aattcctggg atagataatt
    31261 cgaaatcagt tcttttgact ttttcttcat ctataaaaaa ttctttcatc gtccaaggat
    31321 cttttgcttt tgtttgcagt tttataaagg aatcaacaat cgaaaaggac gttattaccc
    31381 ctactaaata ccttgtatta cttttttctc cataaaaaaa tacttcaggt cttgtatcta
    31441 atttagatat tgcagtctct atttcagaac taaaattttc tggaattaca agcaatacat
    31501 ctgcatctct tttttttaga atggatttac cttgctcaac tgattctatc ttttgaatgt
    31561 gaactgtttc ttttgtgcgc tcgtcagaac ttgaattttg gataaataaa tctccatact
    31621 ttcctttgtc taggttaaca attaatacat tgtaataaga aggaaatcca accgaaatca
    31681 tccaatagat aatcacaaaa aaaggaccaa gggaaatagt aattaagaga gaccaataat
    31741 ctctccactg caagattaaa ttgcgtttga atagactgag tgttttcatt ctctaagcct
    31801 ccttccggtc aattgaataa atatatcttc caatgaattt tctcttactt tcatttcaac
    31861 aatagagaga gaatttttct taagtacttg taagatatca gagagttgtt cgagtacatt
    31921 cgggtcagac aaaatacatt gattgtgaaa cctttgtatt ttaatagagc ttcgagagga
    31981 taatacttga tttgccgcaa tggcaatttt ttcggattca aattgaagtt ctaatacaat
    32041 ctcttgtcta tttttctttt ttaaattttc aatcgtgtct aaagaaagaa gttttccctg
    32101 gtctatgatt gccactcgat cacacaatct ttcggcttca tccatattat gaattgttaa
    32161 aattacagtt tttatttttg cgagagattt gatatactct cgaactaaga tgcgactctg
    32221 tggatcaagt ccagcctctg gctcatctaa tacaataatt tctgggtcat gcataagagc
    32281 taaaattata ttcagtctac gttgcattcc tccggataac gttcctgcta atttattttt
    32341 ttgatcgatt aaacccatct cctctaataa atgaagagct ctagtttttg cttggctcga
    32401 atcaatttca tacatctctg caatgtgaag caattgctct aaacaagtaa gcctagtcca
    32461 aagaataata ttctgtggac aaatgccaac tctaactcga agagatttat ctgatttcag
    32521 aatttccttt ccttgtaagt ataaggaacc agatgtagga tttaagagtc cacaaatcat
    32581 agcgattgaa gtgcttttgc ctgccccatt gggacctaaa aatccaaaga tttcaccttc
    32641 atagacttga attgataggt cttttactgc gatgaaactg ccgaattctt ttctgagatt
    32701 ttttgtttct aatactactg tagacatata ttaaattcca aaagatggat ttacttcgat
    32761 ctgtagaaaa cttaacaaat aaaaaaagag aaatggaaag ctttgacaca aatcagtatg
    32821 ttcatagaat acattttcat aaaggatttc ttaggaaaga attttttata tctgaaattt
    32881 atttttaata tacgttatgc gatctagaaa ttgaaaaatc aaccaaaaag atctgatgct
    32941 tgccttgtat caaaaattta aaaataaatt agccacaaat agaaaagcct atcaaatcta
    33001 tagctcaaaa agagtaaaaa tttagaagaa agaacactca atctaattca attcattcag
    33061 tagattcagg tttcattggt tccatagaaa aattcaagag taaaaataga acgattctca
    33121 acataaaaaa gccttgtcct tccaagactt agatttctaa ttgaactagg cggactgtta
    33181 tatgctgatc gtattttttt ccaagaatgc agaaatggct caaaagattg agcaatcttt
    33241 aaacaataaa gtgtttttag attttgaatt tgtttttagc aaagatccag cagaaacgat
    33301 tcatttttta gaatattcca tgcctcagtt tgctattttt gatctagacg atacagattt
    33361 ctcaaatgca aaaaattttg aaatcattgc acatgatcat tggctgagta acactggaat
    33421 ttttggaatc aaagaagaca aaacagaatt atccgcttat gactttcctt tcttttacat
    33481 tttctctcct tccgaaattg tatcacaaat gcctaagata ctttctgttc ttatgcgaaa
    33541 tgaaaactta ctctttagag caggaatcat tcaagatatt ggaaacaagg gaatatttgt
    33601 gatccaatca gagacagaag atgtgaaagc ttacgcggag ttaatcgcaa gtaacctctc
    33661 aaatcgagga ctcattcaaa cacaaaaaaa atacgcagta atctttgctt taaacgaaat
    33721 gctaatcaat gcaattgaac acggtaattg tgaaattaca gcagaagaaa aaaaggcatg
    33781 gattgcaagt gatagaaaaa taaatgaact ggtagatatt aagaagaaag atccaaagat
    33841 tgcgacgaga gtcgttacac ttgaatatga acttttagaa agcaaaattt taattacaat
    33901 cacagacaaa gggaaaggat ttgattatga atcctttcta aaaacagatt tattaagcgt
    33961 tactggttat agtggtagag gaattctgat gaccaaatgt ttcatcgatt ctattcaata
    34021 ccacccaccg gggaataaag tgtcacttga aatcagctat gatacagcta acatgaagtt
    34081 gccggtaggt ctagaagaac ataaaatcat tgatctttct gcaggtgata tactattcca
    34141 aaaaggagac aaaagcaatg gtctctactt tattgttcgg ggcgaatttg aagcctggat
    34201 cgatggaaaa attgtaagta tattagacag aagcgatgta tttattggag aaatggcatt
    34261 tttacttaga catagaagaa cggctactgt aaaagccaaa acaaattcac aagttatatt
    34321 tatcgaagcg aaagaatggg tggacacaat tcgcaagttt ccgatgtata gcgtctatct
    34381 ttctagaatt ctagcacgca agttaaacaa tacatcaatc actgtttctt ctttgaaaca
    34441 gatttaattg cgcctaaata acgccagtgc gatacaataa aaaaaaataa aagattacca
    34501 ccgatgagca ccgataactg aacgatacag caggattgta aattaacgcg aattcgatat
    34561 aaggatttga caaattattt agaccatctt tcccgaaatt ccacccgcta tcgccgcaac
    34621 cgtcaagcga tatcgggaat ctatagttct agatacctcc gataatcgcc ccggcggtac
    34681 tggatgcgaa tagcatcggg tggaattcgg gggtgaccaa gagaaacata tcttacatcg
    34741 aactcacgtt aaattatcat atagttttat cggtgtcctc ggtgtttatc ggtggtaatc
    34801 ttttaaatct ttttacaatg aattcgagtt acttacgaat aaaaggaatg ctaacagtaa
    34861 cagttgtccc ttcatgcatt ttagaaatta catcaatacg tccattgtgt ttttgaatga
    34921 ttccatagga aatggacatg ccgagccctg tcccctgccc gactaatttt gttgtgaaga
    34981 atggttcaaa gattctttgt aaaacatctt ctgacattcc acatccattg tcttcaatca
    35041 ccacttgtaa ttcgtctgat tcttcatttt cttgtaaggt taattttatt ttgccgctaa
    35101 actgttctaa ggtatcaatt tgcttttgta atatagcatg acaggcattg attaatatat
    35161 tcaaaaacac ctgaccaagt tgcgcgggat aacattctat ttttctcttc acaaggtaat
    35221 gagaggtaat cttaatcgat cttttgtatt gagtttttac aattctaaga gttgagtcta
    35281 gaacttcaca gaggtctgct tctttttttt ctgcttcatc gagccttgaa aaagatctta
    35341 gatcctgaac aatcgttctt attctttcac ttccttctag tatattcgaa agtgattttt
    35401 ggaatttaga aaagttttct tcaaagtatt gaataatttc atcttcactt cctgccataa
    35461 gagaaatgac ttcttttcta aacttatcaa tatctctttc taaagctcta gaactaagtg
    35521 atatataatt agttggatta ttcacctcat gagcaactcc tgctactaac gttccaagtc
    35581 ctgccatttt ttctgcttga acgagttgtg cttgtgttgc ttttaattga gtatgttgtt
    35641 cttctaattg agccagggtc ttttttaatt tttcttctgc tagtttatgc ttagtgatat
    35701 cagtagaaat cccactcagc gcatagattg ttccatcttc tctatacata ggaagcttga
    35761 cagataaaaa tacattggtt gttccattga gtcctgtatt gacttcctct tcttcaatac
    35821 gttgcccctt ttcgataatc aatctatcat ttttgcgaat attaatggca gtcggataat
    35881 caaaaaaatc aaaatcatct ttattgataa tatcctccat tgtctctcca aacaaatctc
    35941 ttactttctt atttgcatat tggtaacgat agtgactatc cttaatatag atatatgctt
    36001 ccacactatc taaaatagtt gcgagtcgat tctctttctc caaaagttca ttttcaatga
    36061 gatgttggac tttcatgtct acttcccatt ctgcgttttt cttttttaga tgagacatgt
    36121 cacggctaat atgaatgagc cgttctactc taaaaaccac ttcttcttcg cgaagcggtt
    36181 tcagcaagat atcaaaaatg agatcagact ccattgaaaa taaattctga gagagaaaca
    36241 atacaggtat ttctttctct tttaattttt tataattttc actcgatagg aaattttctc
    36301 gatccacctc ttccacaatc actagatcga ttggattgag cttaggcgga aattgcaagg
    36361 aagcaaaagg aatcttttcc tcgattgcat aacctttttc taatattttt tttacaagaa
    36421 attcttttga tacaagatct ttgtctaaaa tgagtatttt ttttaaacca gacttgtttt
    36481 tacttttgtg aggcattaca ccaatctatt taacctttaa accagatttg atttctacca
    36541 ttttgttttg ctttgtatag atttgcatct gcatttaaaa taagttcttt ccagagagat
    36601 ttatcctgcg gcatagttgt gaatacgcca acactgattg tgacgataga cgctgtatcc
    36661 gacttctcat gagggactgc ttctttagta atcgtttgaa tcaaattgtc tgcaatttga
    36721 atcgcttcct cttgtgttgt atgaggaagt acacaacaaa attcctctcc cccatagcga
    36781 gcggcaagat ctgttttcct catatttttc ttgattgatt ctgcaacaat tttgatacat
    36841 ttatctccca tcgcatgtcc atagaaatca ttgtattttt taaaatgatc tatatctagc
    36901 attagaactg aaatcttttt atccttgcga attgctttat cgcattcttc ttgtgtgaaa
    36961 gattccaacc atctacgatt tgcaagacct gtgagtggat cagtgataga aagaacctct
    37021 aactcattta caagtgtgtc ttggaaatta gacaaatatt ctagctcttc tttttgtctt
    37081 tgtagagtgc gcattgattc ttctagttgt tctgttctct ctcttaccaa ttgctctagg
    37141 ttctcattca tctcttttaa tttttctatg agaactatat tttgtttttc taattcatat
    37201 gcctctaacg cacgctgaat cgaaacaagt aaatctttag gctcaattgg ttttgtatga
    37261 aatttataaa cctgaccttc attaatagaa tcaatgatat cattgatatc agtaaaacct
    37321 gttaaaatca tacgaacggt cttcggaatg attggaatcg tttgctttaa aaactctact
    37381 cctgtcatgc caggcattct ttgatcggaa attattagat tgattcgact tgggtcagct
    37441 tggcttttga taaaagcaag cgcttccatg cctgtatacg cctttatgat attgtattta
    37501 tcctctagaa tcatattgag cgcttctatg tttgccggtt catcatccac gagtaaaaca
    37561 gtgtattttt tttctgtatg gtttttctct tggatttctt ttagtttttc tttatcaatt
    37621 actagtttca tttatatttc ctagatttct actggcattc aagaggtcaa agagctcatt
    37681 gattttgcaa acggaacgta atgagcagat tccaaaatac aattgtgaat gaaaacccta
    37741 atacttaacg actagaaaaa aattgctcaa tctatcctaa gaagtcaata tttttttaga
    37801 agcggtcggt aactctaaca gaaatatttt gctaaaattt cttgtattgc agagcaaagg
    37861 ctttgaattt ctttctctgt agtaaaaata cccgtcgaaa ttcgcactgt ttgattcgct
    37921 tcttccttag aaatccccat tgcaacaaga acagaactcg gttcccatga gcctgcatgg
    37981 caggcggaac ctcttgaaac tccaaatccc cgcttgtcta actcaattac taatttttca
    38041 ttcgagatag gaagcgttat acaactggtg ttcgaaagtc tctctgcttt ttccccaacc
    38101 acttttgcac ccaacgactt caaacgagat tctagaattt tttgattctc tctttggaga
    38161 ttcagatact ctttttgatt ttctttgatc agtgaaatag cctttgcaaa agagagaata
    38221 cctaaaagat tttcggttcc gggtcttatt cccttctctt ggtctcctcc tctaaagata
    38281 ggcactggct tactacgata ggcgaccgct gagactcctt tcattccgta aactttatga
    38341 gaagagaaag aaaaagaatc tgcatctatc tctgaaaaat taaatttcat ttttcctgga
    38401 gcttggattg catcacagtg tagataaact ccaaattgat ctgtaatctc ccttactttt
    38461 tttaaaggct gaatcacacc tgtttcattg tttgcaagca ttaccgcaac tagaactgtt
    38521 tcttcattgc aatgctcttc tagaaattcc aaatcaattc taccattttg atctaccggt
    38581 atccatctca ctagatatcc tttttctttt aagagagttt ttaaattata tatgctagga
    38641 tgctctatcg ctgaaagtaa aacttgcatt ccctcttctg cttcttcctg caataaaaaa
    38701 ttgaaagcta gaaaatttgc ctctgttcct gtcgaagtaa atgcaagttt gtaaatttct
    38761 aaatctaaat cttctagaat ttctcttctt gctctttcga gtgcttctct cgcctgtcgt
    38821 cctgcagtat gcaccgcaga aggatttgca taaagatcca accactctgg aattaacttt
    38881 ttgatttcag gatgaatcgg cgttgtagca ttgtaatcaa agtaaatcat tggtgattaa
    38941 tccaaatagt tatcctgtct ctcgatagaa taaatatcag aaagtaggtc ttgttttgtg
    39001 agagaattca gttgcattgt tttcattcca gctgtgcggg agagtttaaa cttctttgca
    39061 atcttgggtg ttactctttc cattatttca taaaaataat caatgtctat tttgaaaacg
    39121 cggagaggtg attctggaat atttcgaatg ttttctagat caagagattc tgaattggaa
    39181 aagaatcgat ctctaaacat aagcatacaa tacaaaacaa tatgtggatc aatggctggg
    39241 tttgcatttc gtgtataacg tttataatgt gggttgtagg aaagtattcc aagatttgca
    39301 aattgtgagg gataaaataa atctttattc tcctcctttg catacattcc ggttagaact
    39361 tgatagtcat tttctaatga cttctcagaa taattttgtt taatatctaa actttcaatc
    39421 cattctttat aggaacgaat aaacgtctct ttatcaaatg taactaaatc agaatgagta
    39481 aaaaaccaat accaagttgt aggtggattt tccgatccaa tcaattgata gtgtagtatc
    39541 caacttgtag ctagtttatc taaataacga tctcgtttca aaataggaga aataaaatca
    39601 ctaggagaat ctttttctaa aagcccgaat gcatttaccc aatgccgaat cgacttgacc
    39661 atgttaattc caacacccat atagtctgtc ggatactcgt cattagcctc ttctgattct
    39721 ttaaaaaatt cattgactct tcttgtattt ttcgcatgga tttttgctgc tttttcattc
    39781 atccctttat agagccaatg ctctctggga taaaaagttt catgtcctgc tagttttaat
    39841 ttcattttaa tacttctcca aaaaagtctt taatccagat acagatacat ttttttcaat
    39901 tgggtagcta tcttggattg ggcaaataat aaatgcttca tcgatttcta aatcctgaag
    39961 agaaatccaa aatccttttt ctacttttgg ggcagtagaa attttaaact caactgcaat
    40021 ttttctttgt cctttcgtaa gaattaaatc gatttcattt ccactgcttg ttctataata
    40081 attcaattca tatcctttat aaactgcatg gatattttct atacaataag attcccaaga
    40141 actaccacag attggatgac ctaaaagctc attaaaagat tcaatcttta acatagaatg
    40201 tagcacccca gaatctctga aataaatttt tggagatttt acagtccttt ttttcagatt
    40261 tgtaaaatag ggttctagac ttcgcaagaa aaaagtttgc tcaaaaatat ctatataatg
    40321 ggaaattgta ttgtgagaaa ctcctagaga tgcggctaac tttgatttac ctacaacttg
    40381 tccatttgaa tgagccatca ttgttaaaaa tcgagtcaat tgcaacgaag ttgttttaat
    40441 cccaaattga aaaagatctc gctcaatgaa tgtttttaga aaattctccc tccatagatt
    40501 agaacctgaa tcttctttta aatagctgag cggaaatcca cctttccacc aatgctcttc
    40561 taatttaaaa tcagtttgcc cacttacttc taaaatagta aagggagtca gatattcaaa
    40621 tgctattctt cctgcaagcg actcagaact ttgctttaag agttccggag aagcggagcc
    40681 taagataaga atctgtgcat tttttttatt tttatctaca atgctacgaa gcactggaaa
    40741 taattcaggt gtatgttgaa tttcatctaa acaaaccaaa cgattcgcat tcgactcgaa
    40801 gaaaagctca ggattttgga gacgagccaa atccgttgta gattccaaat ctagatagat
    40861 cgaatttggg aatctaccta aaatctcctt cgcaagagtc gattttcctg attgcctagg
    40921 tcctaaaatc gctacaactg gaaaattttc caatgcaaat acaattgatt tttcagcctt
    40981 tcttggataa gtttgcatat tgtaagtcga acttacaatt tacaaggttg tagtcaaatg
    41041 aaaattattt tgagagttca acattcacag ggttccagac actatactct ttataacgaa
    41101 gaattctaga ttcaggaact gggcaggcaa gaatacattt cggtttccat tttgtaaaga
    41161 gaggtgccac tttgtgaaaa tttaattttt cctccgaagg tcgttctgcc ttttttactt
    41221 ctacaagtgt tatctctttt gcactttcca atagaaaatc tatctctact ccattctgat
    41281 ctcggtaata aaaaagatta tatccattct taaaaaatct cgtttttaaa atttcagtaa
    41341 aaacaaattt ttcccaaatt ggaccattca aaacagaatc tttatagtcc gcctccgagt
    41401 gcactcctaa taaagaacaa agtaatcccg tatcagaaaa ataaagcttg ggagctttga
    41461 tcaaacgctt tccaagatta ttataataag gaggaagtaa atgaatcact ccacttgtct
    41521 caagtgcatg aatccatttc ttcgcagtta ctccagaaat tcctacgtca cgcgctagat
    41581 cagtaaatca ccattcagat aatctaaagt ctgagtttag attatctgaa tgtcaagttt
    41641 aaattatctt aatggtaaaa tttcttttgg atttagattt tgctcaatgc ttggttttgt
    41701 ttcacttctt ttaaatcaca cccgttgcgg ttcatgtgga gttttttatt ctagtcgtgt
    41761 tagttcgatg cttatttacg aagttctttt tttatgttat tggaaattaa atcactgaga
    41821 cccataacaa taagatagat actgcaggca atggtaattg taaaaactat taatgtataa
    41881 acactcatag acttcttctc tctaaagagt aaaactttta aaaaatgttg tcaaggtgaa
    41941 aatgtcggca tccctgcctg tttctagaac aagtagggat tgctatcgca aatctaattc
    42001 aaaaatacta atcgttaaat cctaaaaaat cttttccatt ccaacaaata acaaatgaag
    42061 cttagctaat tcttctctat cttttacaat tacacctttc gtccagagtt cattttcact
    42121 caaatcacac tcatcatccc aacttattcc atatccgccg gaatctacct ttgccgtttt
    42181 caagtaagaa agatttttta atttggtaaa tgattttttt tctagcaatg gacctatatc
    42241 atatttcttg atagtgttat ctacaaataa tacataaata ttctgtttgt cgatgaagat
    42301 tttattgatc ctaggaggtt ttaattctgt tggattatta tcattagcca ttagtctagc
    42361 tccggtaatt taataaagtc tttcgtttcc cacattctat ttagatctgt ttggtattag
    42421 ttatttaatc tttaattttt tttacaaatt tcctcttggg tttcttccat ttgcattgta
    42481 gtagttttgg atatattcct cttcaatttc ttttgctttg gatcgtcccg atatatttgt
    42541 caccaatatg cgtgcaaaaa atcgtatcca ttttacaaat gtgttcattg cattcacttg
    42601 tcgttgggca cgaggtgaaa atccatcagg cgttaattcc tcacccgaaa ttccatattt
    42661 tacaacatca ttgtcaactt tatcgattat ctcgtaaaga tggtgagagc tttcgttttg
    42721 attagaatta ccgtgttctg ccatttgtta actatcaata gatagaaaca ggtttttcca
    42781 tttcccatgc tgaaaaattg gaataggttt cgtaggaaaa ttttgaaacg tacgacaatt
    42841 cctgtccagt aagaatgcga taggctcttt ctccaatgta caataacgat tcaaaacttt
    42901 gcgatccaag taaggaagga tttgccaaaa ctttttgaaa atgctcttta cctgaagcga
    42961 tagcaaaaca acgcatatat aaaaataaat ctccggagaa cggtttatct ttcgaatagg
    43021 aaaattcacc caagtgcatg gcaaattttt ttccatctaa taaaaatagt ttttccgcta
    43081 ggatttcttc aaattgaaaa atgtctgcaa cagtgaaacg agttaatgca ttaatgcaag
    43141 gaagaatttg caactttgac tcggactgtt tccaatcaag taaagaaata attttccaaa
    43201 aatcatcttc agtaaaatct gatttatttt cagaagactc ttccagaaac gtaaccaata
    43261 ccttagtacc agaattcaca tgaggagttt ccgacaaccg aacctgtcct ttttcaaaaa
    43321 taccattaac tgttagcata gtctacaata tacctaccta cagattcttg aaaactcttt
    43381 tttatttggg aaaaacatct tgcttggctg tatccaattc atcataaaat ttttttaaca
    43441 tggcaagcgt tattatttgt ttttttaaaa actttgtttg gatttgaaaa attgcaagtt
    43501 ttgataataa tcctttacga ctttgtcaag tcgacagact ctttatcgaa tttactta
//
LOCUS       DAOQOS010000040        43100 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_353_length_43100_cov_6.242421, whole genome shotgun sequence.
ACCESSION   DAOQOS010000040 DAOQOS010000000
VERSION     DAOQOS010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 43100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 43100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43100
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_353_length_43100_cov_6.242421"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(109..942)
                     /locus_tag="PLF66_10650"
     CDS             complement(109..942)
                     /locus_tag="PLF66_10650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HMZ64545.1"
                     /translation="MRPSLFLFALILLFCNCLSASNKKIDEVPKEIAIEPKSDYIPLP
                     ARVTAKPMMNFRELPDGNSKILDSIPEGYVVYTIERTETKENIAGHEDYWYKTKFKNK
                     TGWLFGKYLEFEEKDVSITKIVQPSAKTPSYPTNYKKLRKYFDANMIQKNYKQVIAEY
                     GKPTSELKYKGSNPHGGFNSYLEVVYPDFRFKFIDQILYDVTFFTSEKLKSKTIQIGS
                     ERQELEMEFEVPYYISKDEFSYLTCLPHSEDCPTGYPNAVNFLLENHKVKEIRLTVYM
                     D"
     gene            complement(1070..2551)
                     /locus_tag="PLF66_10655"
     CDS             complement(1070..2551)
                     /locus_tag="PLF66_10655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HMZ64546.1"
                     /translation="MNVKATILILGLSFFSLFAEDEKKVVPVQSSDIEQLSDTFENLL
                     AKSLEDVENGDDDLDIDDISANSAINPKNNPKFPKKTNSTYKPSAPSNNSQFAMPAYE
                     DMDPEKQKLLTKEGLKAIEVAEAKNNKNSVKQIARILLTNPIPEVRSEAARALGRMGR
                     GAKALHRAIDTDAYEVRQHAYKALEKIGSRSSLKYFIKGTKSSDVDIKIASFKGLGKT
                     KSSLGRDIILRVGLNSRDPNVVASALDGLGNFSRKDDLEILRKFLKSDVAEHQSGAVR
                     GLGNSKIPESLDILSTAITENPGLEPEVIFAISKKKTLNATLLLMKMMQTNKNENYQA
                     IIQRELNLRKAYGRYAIVKTNSATIRKQPKASSEKVLVLANADVAKIRKVTEKRFKVK
                     MNNQVVEDRYYLLQAITNKEGSKKGIVEGWVFGAKINVVNIANPSKKGMEDEYGEEDN
                     EDEKDMVKDNTINPEPKKNVKPVNPSKNGKDNDYYEEDEDEDD"
     gene            2754..4277
                     /gene="hflX"
                     /locus_tag="PLF66_10660"
     CDS             2754..4277
                     /gene="hflX"
                     /locus_tag="PLF66_10660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012387099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HMZ64547.1"
                     /translation="MDIARIMGEIARDINRQIGILIDRTGYISHVMVGDYSSIEIPYL
                     DRTRSTGNRLRGLRLIHTHLKDEKLSEEDLADLVLLRLDYITAIIPDEVGNPKFFYSA
                     HANADPESDSLWVVLDKQYPGQLKTGFADEIQDLEFNLSKFKSNLKEASGKNRAFLIG
                     VQFQNQKYHRSIESSIHELKELCRTADIHVVESFYQKKKSLDPATVIGVGKIKEILLK
                     AVQKEVELLIFDLDLSPSQAKKISDFCDLKVIDRTQLILDIFARNAKSRDGKLQVELA
                     QLKYLKGRLSELDDNMSRLTGGIGGRGPGETKLEIGKRRVEEKISRLEKELKSLRQRR
                     ELNRKQRKRNEIPVVAIVGYTNAGKSTLLNVLTNSDVIAENKLFATLDPTTRRIRFPE
                     EREIILSDTVGFIHDLPPDLTNAFKATLEELGDADLLLHVIDISNPNHNEHIQSVENI
                     LSSLELLDVKRINVYNKIDQMEEDFKTPKDGIGISTLNRIGLEKLLETIEKVLWESS"
     gene            4262..4939
                     /gene="surE"
                     /locus_tag="PLF66_10665"
     CDS             4262..4939
                     /gene="surE"
                     /locus_tag="PLF66_10665"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HMZ64548.1"
                     /translation="MGIILTNDDGIDAPGIKALADAVNGNKKIIAPATHHSGCGHQVT
                     THKPLEIQERNDSEIAVFGTPADCSRIGITQIFTNAEWVLSGINAGGNLGVDLYISGT
                     AAAAREASILGKKAIAISHWIREGKPIDWKRATALTSLVLNTLFARELPKKHFWNVNL
                     PHIFEGEKDPELIDCIPSTDPLPVEFRREGDRFIYTGEYPNRKREANTDVAVCFSGNI
                     AISLVKV"
     gene            complement(5100..6467)
                     /locus_tag="PLF66_10670"
     CDS             complement(5100..6467)
                     /locus_tag="PLF66_10670"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HMZ64549.1"
                     /translation="MFILILFIVLSCNEKKQAPEALVTTVIGDVRMGDKKIKSGDVLK
                     NLDSIKTGPKSVCDLQLTGIESNVSIRVKENSEFSLATATQRDSNNVQMKIVSGKIMV
                     NSDKLNSRDNLETITPTVVMAVRGTKYEVEMGSDANGNVAVLDGKVATRVVIPEVENL
                     PPEVRAKSATLKKLDSFLKEKEMVLQTGNATSVSRKQSDTILKETGLGDVLKNADPSK
                     IADDIDTKLSPEKLSEKTAKISDKDLQPEVVQVSPEEMEKKLKEYDEFKPVSKNAIPS
                     SEVKEPEKEVEKPKPEPTKKTEPKIEKKTEAPVNPAPPAKVEEQKVETKKVEEPPVVE
                     KKQTETNEPDQKALDEKSKINLDAKTWGPEQKKATWVEAKKKCDSLGMRLPSKAELLE
                     IYRSQGVFKDWQYINYWTGEEFESNSIFSGKKVMVWVFDMSNGEGIESGKADKDEYIF
                     RCRKK"
     gene            6970..8907
                     /locus_tag="PLF66_10675"
     CDS             6970..8907
                     /locus_tag="PLF66_10675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64550.1"
                     /translation="MSTQAELEFKYSFLKDVRKEKDAILMGINPTNKYQHELLKSIKT
                     LNTIQHATKGMLFNINNTILEQLQIDMKTAVTNLASLVNNQLAAQVCCFEFDAHNREL
                     KVGSFFITHPSEDKSYSIYNLFDELLSYSYNAIKSYFDTREQLLGENFRKELLWQMSS
                     KNADPNSFKGISSLFNPYRRFMESKNGIPISDDMIEYINRELTKRLVESQIAKEVPGH
                     GLLMLKTNEILDHFEAAANAMIEKFIPANANDPTLKSRIDRIVLEEKVYNQVENFPIK
                     TARFAADKAKEIHQYKTIAPGKANSIYPGSLCIQSIISLESAAEERYAIAWRDECNKM
                     KQEFKKNITVPSNKWSKLILFVNHSDSLEIHPEVWKDLINDKDLFYIKWQMPKNTIHV
                     FTGKDPNFFKVLVTGMLNLPANDMWKASALKALIEKYEKQLRSLMTDQSFFQVYQNLE
                     KRIYMQYMPWYYRIFLYFPFSLFQDVILVNAKKKISVEQEVFSSKNDAHNLKYISEMQ
                     SKKMEKSIKIKEQALFESVIETLDLFYLTMKKIPTVNDVKNYFPDMEAFSNIINTRSF
                     RILNLPLKGSEETEVLLYPDNNDWQDNKKALIVKALDSIINDRNPHLVVANADKAKIE
                     KAQKLLNAIEGNSAPKLKAAS"
     gene            complement(8900..9469)
                     /locus_tag="PLF66_10680"
     CDS             complement(8900..9469)
                     /locus_tag="PLF66_10680"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HMZ64551.1"
                     /translation="MKLKIHYEDIKTIYSKEEILNQIETISNSIAAFYNDLPVDYFFQ
                     SPLDGGWSPEKNIRHLIKSTQPIYLGLSSPKVALLLFGSGKDKSRTMSEIKSAYLAKI
                     DAGGGAGIFTPVRENTTPDMVIQQKLIQDFTKLFGKYKKQIENWKEEDLEKYKMPHPL
                     LGNLTVREMVLFSIYHLFHHTEKVEHRFS"
     gene            9784..10107
                     /locus_tag="PLF66_10685"
     CDS             9784..10107
                     /locus_tag="PLF66_10685"
                     /inference="COORDINATES: protein motif:HMM:NF018050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HMZ64552.1"
                     /translation="MFLADSVLVDSIQDLKENKSVNKLGFNLFKIRISSGNKGKRGSF
                     RTIVVYVSNIRAIYVYGFSKNELENISNKELEDFKELAKTYTNMNEMEIKLLLEQNSI
                     FELEE"
     gene            10110..10448
                     /locus_tag="PLF66_10690"
     CDS             10110..10448
                     /locus_tag="PLF66_10690"
                     /inference="COORDINATES: protein motif:HMM:NF027067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system MqsA family
                     antitoxin"
                     /protein_id="HMZ64553.1"
                     /translation="MTNKIKEAIADNLQDIVNAGIETTFTKKRLKELGVKISKTSINP
                     NKIKRVRKNLNVSQSIFAQLLNVSIASVRHWEQGLREPTGSTMVLLELLSKEPHILDY
                     RITKSKRIAV"
     gene            complement(10613..12055)
                     /locus_tag="PLF66_10695"
     CDS             complement(10613..12055)
                     /locus_tag="PLF66_10695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64554.1"
                     /translation="MRILWIEDFPKESDKPSSLAANLFKDVLGLESFDEDYESLNDIS
                     KELPNYFKIKNSIHEIVVCKSYHEYINTHDLKYLNFDIFIVDINLTDAEPNNSLIPAG
                     FNRKDFLEKSGFYIWNLLIRKGVPEDQIAFMTGNGNSVSDFEKHCRDANIPLLKTENV
                     FEKSEENEDSPKFKRGYISFRKWLQLKASNPYIQLRRGILDGCEYLLKNTPEEILLNR
                     TLKKDGEGKKIDELSQEYGKEFLIRLQGIQQKEFHLDGKEKTQKLYTFLMTLVSEWER
                     GESKDAEQKEHFKDISDEDEKTFYETSFWILNNIRNCFAHFTFDKENLNEQDIAFFFI
                     LAIRGFFKLDINDTLDYEKILFSIMPKANEIITKEQIEKQNSKSLENIKKLFTDYKLD
                     EAWRRLPKKGQQGERINKPSDYKGYALRFLDGAKQNHNAALIPIPKTEIQKESKSIFY
                     NTYLHGMGSFLTEANKEKVYYRLANYSFST"
     gene            complement(12036..14024)
                     /locus_tag="PLF66_10700"
     CDS             complement(12036..14024)
                     /locus_tag="PLF66_10700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64555.1"
                     /translation="MNKAILYYQKYLKLNKENEDICIKIFNYYIQNEQYEKAESIEIS
                     TGNDFLFFWSNFIFPLVVARGENRIDLDKFKNLFNLNYHGLCQFILNTESESKVVHLF
                     YQIVIYVIINNKENEFLKIIDSLLNKFPNNKSILLVYSSLKFILNDFDTALQSHDRLL
                     KEPSLKEEKLKDFISSTLNFFYGFCAFNTHQNNLGINYLELSNIKFPSKLANFILGQI
                     YQIENRETLSLKHYNLVFEYQNNVPEIWPKLTKNEINNLLDEYNIKLGSVFEEVELKT
                     IDLLISIYEKNKMHAELINLFRKILKKDFSDNFYYLVSFHIVRLSKNKLINFDFRNLY
                     TELYNKFNKGNFKSELFAFSLLQNNYDLQEKARQAEKEKEEIEIKTKQTLLSYLSHSF
                     RNSLSNGPETIRETLNLAKKALEKDYEDKLAYKAFNNLAGLYSTFAMVENILDTFQLF
                     VQDSGRFVEEWHKEIRGDKSVKYLFSLSVKQNISRFLFSEGLIQEREKLIPNFSSEKL
                     RSLRKSFLEKILFVEFEELSPDLVLSWVKENLDFIKIEISDEITKFNKRGVHFLLNYS
                     VVSESMFNALKYSSNEELIHISWKRENSTILFECKNRFSKESVKYSDSKSGLQFIEYL
                     INKIQKDIRFTRESKKSEFKVTLKLTNIKGDLYENSLD"
     gene            complement(14359..15297)
                     /locus_tag="PLF66_10705"
     CDS             complement(14359..15297)
                     /locus_tag="PLF66_10705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64556.1"
                     /translation="MSNNSLNLPSIRKNDISNLNTKRANIVTLDLSNKSGINQLMKKK
                     SENLKEQFSDKKKGSLDLVLMGDLTGSMATYHQLLKTEFIKLSRELFQFIPDLQIGII
                     FYLDHGQGDPYTTKVCKITNDIQTIQEFIQRTPTGNGGDAKEAVEDALYDAFQINWRP
                     KVNRSIVLFGDASGKEPHECPHQHSYFQITKELYEKQVTINSVYCRPGFSSQSLQALA
                     PIRIGDFQKKVSNLSHPNFFSWLANVTGGMIIGIQEVGDLIEIIKASAAKDAGVLDEY
                     EASISKSTSKKKLELIEISKKANQRKLENSNRLRLE"
     gene            complement(15294..16742)
                     /locus_tag="PLF66_10710"
     CDS             complement(15294..16742)
                     /locus_tag="PLF66_10710"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HMZ64557.1"
                     /translation="MSQNNILQKEPASFFHTNIILSGRYILSRFRTLIPFFSEKKNAR
                     ELLSNSHTLMRDTLSFQDKIFQLEQNIHKSNQSFDSTQIELLKEIRALVKNIEKEKFL
                     VIQQKEFLEKKEMELKEKSKEIRQTEIWITAKKTDIKKELKQIREKEFEIEEREKSIL
                     QETERLHFKESEILEKETNLSDREAFLAEKEIEFSFHERELSEKQELINEKEIELKEG
                     LEKLFKEKEELNKKLAEKIDEYDEKFASLKETKETIDLFKKDKSKEGKEVKLVVQEAI
                     RQMQKLSKDMLSRSEELEEKYCGGTFKGFALPLDEIENKIEELKNHLEEIYSYSKDNP
                     HIPLNLFIKEIELRFKKANESKDLWDFPMAFQSVIEAITFCLSLETFLKSLNEWSESS
                     GEPKEETSEEESDFYADLGVSFDATFEEIKKAYRDLAKKFHPDTQSPDLSEEEKEENR
                     KQFFKIQKAYEVLSDESSRREYDSKKEKGEKI"
     gene            16858..18948
                     /locus_tag="PLF66_10715"
     CDS             16858..18948
                     /locus_tag="PLF66_10715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016549575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HMZ64558.1"
                     /translation="MNSNRKPNRLIEEKSPYLLQHAYNPVDWYPWCEEAFEKAKREDK
                     IVILSIGYATCHWCHVMEKESFEDERTAEIMNRDFVAIKVDREERPDIDKIYMDALHA
                     MDQQGGWPLNMFLTPDKKPVAGGTYFPPVQKYGRKSFTEILAIIKNVWDTRKQELIDS
                     SEAIASYLQKENLADNPDLTLPPDTAFSSAYQMYDRFFDDTYYGFKTNMVNKFPPSMG
                     LSFLLFHYHITKETKALHMVEKTLMAMKRGGIYDQIGGGLCRYATDGRWLVPHFEKML
                     YDNSLFIEALRECYQVTQNPFYKEVVYDIVKYIRRDMTLKEGVIASAEDADSEGEEGK
                     FYLWSLEEFRSICKEDSEVLEKFWNVTEEGNFEEKNILNETFDIDFIKENNLNREEFQ
                     SIVQRNKEKLLDVRAKRIRPLRDDKVLTSWNCLYLKAITGAAIAFGDKSLLADAIQIF
                     DFIQKNLFNENCRLLRRYRTGESKILAYLTDYAELTLASIYLYKATYDLKYIKTAAGL
                     AEETIRLFQSEKGVFYDTGIDGEALIRRSIDAYDGVEPSGNSSMAHAFNFLSSVGIET
                     ERYENIANSIFRFFSENLVKNSISYPSMLSAYYYTKSSPKQIIVLAEKENKEAQEILN
                     FLEKEYIPNSILLYVEKENLSEAEKVLPILKGKNSDKEVAVFICKNQTCDLPIYSLEA
                     VKKAIGVFTTDKHG"
     gene            complement(19055..20557)
                     /locus_tag="PLF66_10720"
     CDS             complement(19055..20557)
                     /locus_tag="PLF66_10720"
                     /EC_number="1.11.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF012425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HMZ64559.1"
                     /translation="MNRHTTSSGMPIGSDARDGILETHLLNEKLAHFNREKIPERITH
                     AKGAGAYGVFTVTADITSYTKARIFSEVGKHTPVFVRFSNYRGEKGTADTVRDPRGFA
                     IKFYTEEGNWDFAGNNIPIFYIRDARKLPDLIHSQKRDPQTNLNIATSMWDFWSLSPE
                     TLHGVVMNFSERGIPKSYRYMDGFSCHAFSFINAKNEKVWVKFHFKSEQGNKSLSSEE
                     AIRIAGENPDYYTEDLFQSIESGIFPKWKLFIQVLTDKDLEKFTFDPFDVTKVWPHSI
                     APEKEVGILELNRNPENYFAEVEQAAFSPANIVPGIGFSPDKLLQARIFAYPDAQRHR
                     LGVNYQTIPINRPRSYVENGYRDGMMRIDSNGGSHVNYSPNSLEEMNERTLQPSANGN
                     DSLAFNKTYPLDEYTQAGNLYRLMTDEKKEILIENLVESMRHIPTRIQIRQCVHFYKA
                     DTELAYSVVDSLGLDFNEIKMLSTFTYEELIAETAKALYEKIERAKTTAK"
     gene            21114..21494
                     /locus_tag="PLF66_10725"
     CDS             21114..21494
                     /locus_tag="PLF66_10725"
                     /inference="COORDINATES: protein motif:HMM:NF013595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64560.1"
                     /translation="MQSIYKTISILSLIFSLIACNSKTNNDKTILAAGLLFLQNQKSN
                     SGIGGPTATRVYAQLGSFTTNTTNKGGVSANSFYAPNGIALDSSGNLYVADSSNNRVL
                     YYPSNSTTATRVYGPIMKPNVYFI"
     gene            21598..22356
                     /locus_tag="PLF66_10730"
     CDS             21598..22356
                     /locus_tag="PLF66_10730"
                     /inference="COORDINATES: protein motif:HMM:NF013595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HMZ64561.1"
                     /translation="MIVDSSGGVYISSSGRVLYYSSGSTTATRVYGQGGNFTTSTINL
                     GGITANSLSGPAGLALDSNNGLYVADYSTSRVLYYPSGSTTATRVYGQLGSFTNSIVD
                     PRNVTADTLYTPTGVIVDKSDNLYVADTSNNRVLYYPSGSIVPTKVYGQSDFTSSSTL
                     NTSNSSLGGPNFLTLDSNGGLYVSTGENRVVYFPSGSTTATKVYGQGGSFTSRIQNNG
                     GVSANSLSNPTGMAIDSIGRLYIADFGNSRILYY"
     gene            complement(22774..23136)
                     /locus_tag="PLF66_10735"
     CDS             complement(22774..23136)
                     /locus_tag="PLF66_10735"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HMZ64562.1"
                     /translation="MKLIDSNILIYSSLDEFTYLKEYIQSENSISEISILEVLGYHKI
                     TEEEKIYFKSAFTILTIIPLNRNIIDKAVELKQQRKLSIGDSIIAATALILSLDVLTR
                     NEDDFSAIKDIKVFNPIK"
     gene            complement(23133..23360)
                     /locus_tag="PLF66_10740"
     CDS             complement(23133..23360)
                     /locus_tag="PLF66_10740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64563.1"
                     /translation="MKLTLDIENEQDLFLILSFAKRVGIKILDKDNSVQDNLEYHRKI
                     ISQGVKKSSFGDPMLWQRETRKDRNLPLRTE"
     gene            complement(23793..24188)
                     /locus_tag="PLF66_10745"
     CDS             complement(23793..24188)
                     /locus_tag="PLF66_10745"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain nuclease"
                     /protein_id="HMZ64564.1"
                     /translation="MILVDTSVLLDYFSGNIGAHVIKFDEVLKSKIPFGINSMIYMEV
                     LQGASSEKEFKTLYEYLETQKFYELKNLDSYKKAAEIYYKCRKSGITVRSTIDCLIVQ
                     TCIENKLHLLHNDKDFIHISKLIKELKFY"
     gene            complement(24185..24385)
                     /locus_tag="PLF66_10750"
     CDS             complement(24185..24385)
                     /locus_tag="PLF66_10750"
                     /inference="COORDINATES: protein motif:HMM:NF021474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HMZ64565.1"
                     /translation="MRTNIVLDDELMEVAFLYSGLKTKKEVIHQALLEFVENRKKKDL
                     RDLKGKIQIKKGYDYKSLRAGK"
     gene            complement(24472..24774)
                     /locus_tag="PLF66_10755"
     CDS             complement(24472..24774)
                     /locus_tag="PLF66_10755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64566.1"
                     /translation="MEKENLKLIYDDEYNKLYFDEVNQYYVLWLMISHVMWYDIYIRL
                     IDEEVKKILKPDGEINEVEFRHFANDIALCRPGLVGRYTPWDERILYRKNIEKEIE"
     gene            complement(24774..26093)
                     /locus_tag="PLF66_10760"
     CDS             complement(24774..26093)
                     /locus_tag="PLF66_10760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HMZ64567.1"
                     /translation="MLEIPGFKNLRKVLWKDLEAGMIVVGGVKVNNGLPLELAHFPAL
                     NITLIRELTTKYHFLPNREVIVAEVQKGESASRMSMGFRTIEKRLKQFNDFRDEIQRI
                     KKNLIENQGLILPANTLLTSTSYVNQDHLIKDTYNSFEIKIASSNPPSMFSHLEEKIT
                     LADILSGRLKDKFRLPEDVEVMLHLVVDYSYSMNTMDKLDKVISAVNLFYTHISECML
                     NVKLQLYVFSETCIPAKFPLSGKEIERKGTHYGSFMKKVLHHRNPDTINKVILFTDGE
                     PTDKTDAVLTGNKMKKLKIDYTQIIFDINEDRRVEYKGLSGSEKIMDGFLVNPPPGIT
                     PIRLNDTDWKNRKAEIYEIFTEVAHACGGNQIIINVNELMSLISVEVYDRYMGSLTLS
                     RNPVVLPHLQEFKVDPRHSPTKESMKEEITKKTIKPFEFKRVERPKQ"
     gene            complement(26235..26561)
                     /locus_tag="PLF66_10765"
     CDS             complement(26235..26561)
                     /locus_tag="PLF66_10765"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HMZ64568.1"
                     /translation="MEIAFRNKGDNKILQFSGNLDIYTSPIIKKQALEKIEEEEVKSF
                     IFDMSKVNYFDSSGIALIANLRKRMLEREGKFALLSISYEIKSVLQLASMDRFFTIYA
                     TEEEIN"
     gene            26947..28218
                     /locus_tag="PLF66_10770"
     CDS             26947..28218
                     /locus_tag="PLF66_10770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008592687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HMZ64569.1"
                     /translation="MNLKSSKLITLFLSLCSLSLSAQVTVGQLFQKPAVIGDSLSQGF
                     YGVTVERKTQDWAYPVLVSKQAGSSVSYNVLKGPYANLEDILKLNCGPICLASAIIGG
                     NESTVALPTHAGITGADYTNALYTSGSCEDIRATKLVKEWYWETWYWYTYRWVTAPDC
                     VTPDKFHQYGLRGSGTQIQIMEKVKPTFLFGTVAANHVLCTALATNLACLDYPRFEKD
                     IRESMRRLAAMGSLKGGVLFTVPNVTAIGYLEQYTDPQNRPGFSGRKAFYRSSVSSAD
                     QVLDETEIATITSFLTKLNNEVKTQATSMGFALADAKVLFDDIKENGRPIKSPSGYSP
                     GSAKANWPLPNQPGLFGLDGVHPNMYGHAVMANELIKAINSKYGISIPQVSEYNAWYY
                     DELNRNPVDLKNFLTNNIFGQAISWLIGIFA"
     gene            28258..28935
                     /locus_tag="PLF66_10775"
     CDS             28258..28935
                     /locus_tag="PLF66_10775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64570.1"
                     /translation="MNKKFLIIGGIALAILAIVVLFKPTTGKKKTAQTNNVSDSDNVS
                     SSSGFSNFSDAPSPSTENPSLEAEAEKLWPHLAKQDNSAKKKERVREEWSAFANKYPK
                     NFYIPAEMKAPMTEAEMAEKRKEVDIVSKIESKIAVVEANARKTEAGSTPPKAPSKPD
                     VTPEEQRIYIGFKTKELESRIQLIEYSIDNSGISGDQLTVARKDIAGWKKELSDLQDV
                     EKKIPST"
     gene            29074..30624
                     /locus_tag="PLF66_10780"
     CDS             29074..30624
                     /locus_tag="PLF66_10780"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HMZ64571.1"
                     /translation="MLIKLLFLFLISLISCKGPDKPDTLPTIKESPMKTKEEIKRDLK
                     STNFYERSQALVEIGKLKEKEFIPEIKQLLKKDPNPAVRGSAALCLAELEDKSSTLDI
                     IDLFKDKDISTDVVLDALTRMKDPQAIPSIIPVLDSSDHTHRLLAVEALTQIGSTNGT
                     DKIITMASANKDIEKAKTYAMVIGKLKLVKGENILLQLADTTEASPTLAATYLALGRI
                     KSKKSVPILAKAIGKDFDKGRENSILSLKEIGDTAANPLLIDYLKNKNKEIQMGAADV
                     ISTLYDLKTAENVAKLLESSDKSVFAPSAYILGRYKYEPSRKKIEAIATDKSNPDREI
                     ITQSLGWLQNKESIPVLKQILQEKEGEGRYGAAWSLGMLGAEDAIDLLEEAANSRDNK
                     LARIAIESLGQIQSPKSLSFLSKKIETNKELSSSILASIASIPGQEATKVLDKYALST
                     DSVIQRSALQAILQRKDKENIPVLIKILEENKGADTASFAQLALRGITGENFSSRNEW
                     INWFYKKK"
     gene            30634..30987
                     /locus_tag="PLF66_10785"
     CDS             30634..30987
                     /locus_tag="PLF66_10785"
                     /inference="COORDINATES: protein motif:HMM:NF025062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CZB domain-containing protein"
                     /protein_id="HMZ64572.1"
                     /translation="MDFDKAIATHVEWKAKLRSYLKNLDSNVDPKIVEKDTECSLGKW
                     IYGEGAKYKDNEYYLQLVSEHARFHICAAAVIREIIAGNISKAESMIGLTSEYTEVSA
                     SIVTKIRKMRDQIEK"
     gene            31044..33614
                     /locus_tag="PLF66_10790"
     CDS             31044..33614
                     /locus_tag="PLF66_10790"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HMZ64573.1"
                     /translation="MFRILIYCLFFVFPVFSDNLNVSLTQKCEQMDCKPRTWYAIDSF
                     DPSFLTLSEIDKSWKEITQFPIWLNKFFTKDGSLATYSLATNFDLPQELLSHKEQIGI
                     RFGEIGEVFEIYINGNLIAKEGIIENDKVSFHRTVRGKVWQVPKEYLKETNNIVLVKI
                     SGHPKFDHTGFYLTKYYDIGLADALKYDEQDRISLALIGIYFVVGLYHIFLYFRRRVE
                     NYNFYYGAFAVIVSLYMYTRTSAIFEFDWDTALIQRFEFAILFPGVAFFVKSLDSLLL
                     NNVNKWSRYYGYFSYSLCGLTLVTPEMYLAEYILRLWQVSVLLCVLPLLIRIFYIAIK
                     NKVSNAKRLFIGMVVFVLSAIYDIVDSAFLNTGLAFSKYTFMMYILGFAAVLADRFIS
                     IHIEIEELNENLEKKVELRTQELSTSLDQIKNLKDQQDGDYFLTSLLLHPLGANQARS
                     QEVKVEFLVKQKKKFQFKEWKSEIGGDLCRSQTLYFKNRPVTVFLNADAMGKSMQGAG
                     GALVAGAVFNSILERTMLSSTVQNQYPEKWLKNSFVEMHKVFESFDGSMLISVVMGVI
                     DNTTGLLYFINAEHPYTVLYRDGETSFIGHEKLYRKLGSSMVDDFISIQTFQLKHGDV
                     IINGSDGRDDLMIEDKTENKKILNYDEKIFLSVVKKGEGEINKIYDKLVETGEFTDDL
                     SLVRIEYRGNNLAIESFDETEIPDVEVSFKANTREALESIDAHLAEFKNSDSKNLRAL
                     KYIIVGYIRLKKFKTAIDLAFEYLEIIPADTEVIYYLSYSFRKDKQFLQAIEEGERVR
                     IRNPHHSKNLINLARSYASLGNFSRAKEIILLAIQINPEDLKSIRLQMQIEKAIRAQS
                     "
     gene            complement(33764..34321)
                     /locus_tag="PLF66_10795"
     CDS             complement(33764..34321)
                     /locus_tag="PLF66_10795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64574.1"
                     /translation="MNFWFLVVSMIFVRSLDAYLTYLITPDLKFELNPLVSVGNQGWA
                     TLLSVNAFVVFFTLLLLYYSFNNSVHIYYPKEKNYTLKEFISFFLYNEKNSFYKIYFR
                     VPYNRKVILNFCGYLFARVFTVWSVIIVSHNLLLLHLENYHFYSSEWNLWVLVYLAIL
                     PITFYFFWKFFRLEYRKYLVNARSM"
     gene            34475..35044
                     /locus_tag="PLF66_10800"
     CDS             34475..35044
                     /locus_tag="PLF66_10800"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HMZ64575.1"
                     /translation="MTLSNPFNKSELKSAIQDLKQNLANSFQAIDEKTFFQKPEKGWS
                     PAENIQHMNRVTKVLGLTYVTPKFIASFIFGETNHKSKTMNEMATIYLDALRQGKDSG
                     FFAAKTEKTDINTTKRKEELIRDWNSEWEKYKTAIDGWSEEDLDKILMPHPFLGKITA
                     REMYMVGILHPVHHASIVANRLGVNWNYF"
     gene            35061..36188
                     /locus_tag="PLF66_10805"
     CDS             35061..36188
                     /locus_tag="PLF66_10805"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HMZ64576.1"
                     /translation="MSEIVVLLSLAVVQTFFSILFIISKKNKSTHSIIASLWIILICL
                     AALRRGLSILFPDESNFFQKVIFYPLAYGPCLYLYTRMQTKNEKTFFKKDLLHFFPFL
                     FFLFLTFISMIRMQNGSIYFERSKIFSVIEGIFAIAACISVSVYNILSYRMIKEHILQ
                     IQDKFSYLSIDNTLSWISRFSILFLIFLINQLVVFLYSTFYGKVLLNPVANVYLLLGF
                     LYTLSFFFMRQDDVYNQTKNFEDIIKKEKYSKSGLSKTKMEQLENILLDYIEKEKPYL
                     NPDLVISDIASGIGISTNHLSQIINTKFEKNFFMFINDFRIAEVIKKMNDSNYDEYPV
                     IRIAYDCGFNSKSSFNSIFRKATGMTPAQFRSKRNRESNTL"
     gene            36300..37637
                     /locus_tag="PLF66_10810"
     CDS             36300..37637
                     /locus_tag="PLF66_10810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002971778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 family protein"
                     /protein_id="HMZ64577.1"
                     /translation="MDVASLDLWFRRGISVITLFFGIVQLYLSEKNIWIDAGFIICMA
                     GIVGYYTNFLAIKMLFQPKQGKVLGWEGLVPKNKANIAKSLGQSIQTNLLAPEIILEY
                     VYERKLIETGTQKVGEWLDELLKDQVFRDKVTEKIISLLQEKGPELLSQIFNFTEETL
                     KDIARNPEEIKKVWEQIRFRINEYLQSRQNREYLVTQTRKIILEELPRLSVVLNNAID
                     EYLKQKDTIGNIGFGIKKIISFDNAALQDLLQKFIEDEDTTNQLMGVMDILVLDLQKK
                     MEAQETQDYIITQVKDWFDVASEYSRKTFLPSAIERLKKYLDDPDNWDKVGNYSFKVL
                     DYAKDKSLEFMQSQEGSEYLKTNIAKIIHQVNVTNLVEEQVMKLDTDELEKMILDNTG
                     GNLVMIQFLGGVLGLIAGFIQVHIYFSVPVLGLVVLTWISYYRNQKKYNIQTK"
     gene            37804..39051
                     /locus_tag="PLF66_10815"
     CDS             37804..39051
                     /locus_tag="PLF66_10815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002088399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HMZ64578.1"
                     /translation="MKGRIYPIFLILFFLVHCDGIRKFLIQKLGAADKYKAEGNIANL
                     SPIFSGQDEKRRKIDITLREVASGFIQPTDMQFPPGESEEFFILEKQGRILWSKVNGK
                     ENKEILSIKVKAESEEGLLGLAFHPEFRKNGKIYVNYVAGVGKKDISRISEFTSTNPK
                     DLQNSKLESERIIMEVEQPYENHNAGQILFGKDNMLYIGWGDGGWKDDPRKQGQNPKT
                     FLGSMLRIDVDSTPDKDKAYKVPEDNPFFTDKCCKPETFAYGFRNPWRFSFDPKGRMI
                     LADVGQDLWEEVDIVEKGKNYGWNIREGSHCFEPKENCKTENLADPIYDYGRDDGQSI
                     TGGYVYTGKNIPSLSGKYVFADFVSGRVWALDLPENPTERVKEAYTLGKWPVLISSFA
                     KDGNGDVYLLDLGKGKVFKIEPK"
     gene            39048..39629
                     /locus_tag="PLF66_10820"
     CDS             39048..39629
                     /locus_tag="PLF66_10820"
                     /inference="COORDINATES: protein motif:HMM:NF025244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4202 domain-containing protein"
                     /protein_id="HMZ64579.1"
                     /translation="MKQELFKQAIVEFDKANSLDPNLITIGDEQKPRELAYSEWLSDW
                     VQRLNPNASEPLQLAARCQHLKRWEIPRSKYPDGLKGYTKWKKELAEFHAQEGEKILK
                     KIGYDPATIERVKEINLKKNLKSDSEVQTIEDALCLVTLAYQIEDFSKKHSDEKMIEI
                     IRKTWGKMSNKAREEALKLKYSERVLSLIQKAI"
     gene            complement(39694..40863)
                     /gene="recA"
                     /locus_tag="PLF66_10825"
     CDS             complement(39694..40863)
                     /gene="recA"
                     /locus_tag="PLF66_10825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010578143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HMZ64580.1"
                     /translation="MKKGKEDKSEEILSEKNEERKRAIDAAMGQIEKQFGKGSIMRLG
                     AQSDVAIPVISTGSLDLDIALGIGGLPVGRIVEIYGPESSGKTTLMLSTIAQAQKKGG
                     IAAFIDAEHALDPSYAKKLGVNIDDLLVSQPDNGEEALEICESLVRSNAIDIIVIDSV
                     AALVPKAEIEGDMGDSHMGLQARLMSQALRKLTGTISKSKTTVVFINQIRMKIGVMFG
                     SPETTTGGNALKFYASVRMDIRKIETLKDKEDPIGNRVRVKVVKNKCAPPFKQAEFDI
                     MFNSGISRESSIIDLAVKHEILVKGGSWYSYNGEKIGQGKETVKNYLLENPQILDKLE
                     AQIRDLNDLPSNKTAGVVSEKETSNGKGNHSEKIEKGSRRNMALEEELESAEAVA"
     gene            41141..41368
                     /locus_tag="PLF66_10830"
     CDS             41141..41368
                     /locus_tag="PLF66_10830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004448221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64581.1"
                     /translation="MKNSRFQPAFKTRDIQLLSILKFHLEEEEIPYFVTGENFLFLEN
                     LAVPSHESYAVLYLLPEDFEKFEKLLEDGFV"
     gene            complement(41388..41720)
                     /locus_tag="PLF66_10835"
     CDS             complement(41388..41720)
                     /locus_tag="PLF66_10835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64582.1"
                     /translation="MKLNQITLFIFFLSFTILLNADDKKWSSLQGSLKWNEAKKKCSS
                     LGMRLPSLEELKTSFDKEELSAWKKDGNTYWTASEISSDRAYYFTLLNGAKFSLAKNK
                     SISVRCVR"
     gene            complement(41925..42467)
                     /locus_tag="PLF66_10840"
     CDS             complement(41925..42467)
                     /locus_tag="PLF66_10840"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HMZ64583.1"
                     /translation="MKRICVFCGAHPGENRAYYENAKCLGEFLVKENFGLVYGGANIG
                     IMGEVANSVLAQGGDVIGVITEHLMPIEGHTGLKDLRILKNMHQRKAMMYELSDGFAV
                     LPGGIGTLEEFFEILTWAQLKFHTKPIGILNTEGYYNRLIHFLHHIADENFMHRQQLE
                     LFFVADTPEIMIQKFKERIG"
     gene            42584..>43100
                     /locus_tag="PLF66_10845"
     CDS             42584..>43100
                     /locus_tag="PLF66_10845"
                     /inference="COORDINATES: protein motif:HMM:NF019436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HMZ64584.1"
                     /translation="MKLFFREFTGNGRPLIIIHGLFGSSKNWITNAKELSKLAHVYTI
                     DVRNHGDSPHTNTHTIQDLVSDLKEFIEANNIDKPILLGHSMGGLNSLLFALTYPDLI
                     HSLIVVDIVPKSYPIKYEKEFQALTMDVSNYESRQALDEDMREKLPDTFIRQFLQMNL
                     EKTETGYRWKLN"
BASE COUNT        14347 a         7325 c         6999 g        14429 t
ORIGIN      
        1 gtctcctttt ggaagcctaa aatcgtgtcc tttttttatg taggggttca atacctcctc
       61 atccgtgcac acccgtattc ataatccgta atcattaacg ctctgcggct aatccatata
      121 taccgttagt cgaatttctt ttactttatg attttctaat agaaagttta ctgcatttgg
      181 atagccggtt ggacagtctt cgctgtgagg aaggcaagtt agataagaaa attcatcttt
      241 agaaatataa taaggaactt caaattccat ttctaattct tgtcgttcgg aaccaatttg
      301 aattgtttta ctctttagtt tttctgaagt aaaaaaagta acatcatata gaatctgatc
      361 aataaactta aatctaaaat caggataaac cacttctaaa tacgagttaa atcctccgtg
      421 aggatttgag cctttgtatt ttagctcgga ggtaggttta ccatactctg ctatgacttg
      481 cttataattt ttttgaatca tatttgcatc aaagtatttt ctaagctttt tataatttgt
      541 aggataactc ggtgtctttg cagaaggctg aacaatcttg gtaatcgata catctttttc
      601 ttcaaattct aaatactttc caaataacca acctgttttg tttttaaact ttgtcttata
      661 ccaatagtct tcgtgtcctg caatattttc tttcgtctct gttctttcaa tggtgtaaac
      721 tacataacct tctggtatag aatccaatat tttactattt ccatccggta actctcgaaa
      781 attcatcatc ggttttgcgg ttacacgggc agggagtggg atgtaatccg atttaggttc
      841 aattgcaatc tcttttggga cttcatcgat tttcttattt gaggcactca aacaattaca
      901 gaatagtaag ataagggcaa atagaaaaag gctaggtctc atacttttta gtctatatat
      961 gattgataga ttgttttgaa aagcgaattt attttcctac tgaaaactta tctagaaata
     1021 aaaaaagcca gcgaatgctg gctttcttcc cctaaagatt tgatttggat taatcatcct
     1081 cgtcttcatc ttcttcatag taatcattgt cttttccatt tttactagga ttaacaggtt
     1141 ttacgttttt ctttggctct ggattgatgg tattgtcttt aaccatgtcc ttttcatctt
     1201 cgttgtcttc ttcaccatat tcatcctcca tccctttctt agatggattc gcaatattta
     1261 ctacgttgat ctttgctcca aaaacccaac cttctacgat tcctttttta gaaccctctt
     1321 tatttgtaat agcttgtagt agataataac ggtcttctac aacttgattg ttcattttta
     1381 ctttaaatct tttttctgtc acctttctga tcttcgcaac atctgcattt gcaagaacta
     1441 gaactttttc agaacttgct tttggttgct ttctaattgt agcagaattg gtttttacaa
     1501 tcgcataacg accatatgcc ttacgaagat ttaattctct ctggataatc gcttggtagt
     1561 tttcattttt atttgtctgc atcatcttca ttaatagaag ggttgcattc aaagttttct
     1621 ttttgctaat cgcaaagata acttctggtt ctaatccagg attttcagtg atcgctgtac
     1681 ttaaaatatc taaactctca gggatttttg agtttccaag acctctcaca gctcccgatt
     1741 ggtgttctgc tacatctgat ttcaaaaact ttcttaaaat ctctagatca tcttttcttg
     1801 aaaagtttcc gagaccatct aatgcagaag cgactacatt cggatctcta gaattaagac
     1861 ctactcttaa aataatatct ctacctaaac tgctttttgt cttaccaaga cctttaaaag
     1921 aagcaatctt gatatctaca tcagaagatt tagttccttt gataaaatat ttgagagaac
     1981 ttctagatcc aattttttca agggctttat aagcatgttg gcgtacttca taggcatcgg
     2041 tgtcaatcgc tctgtgtaat gcttttgctc cgcgtcccat tcttcctaac gcacgtgcag
     2101 cttctgagcg aacttcaggg ataggattcg ttagaagaat tcgtgcgatc tgttttactg
     2161 agtttttgtt gttttttgct tctgcaactt cgattgcttt caagccttct ttagtaagaa
     2221 gcttttgttt ttcaggatcc atatcctcgt aggcaggcat agcaaactga ctgttattag
     2281 atggtgcaga tggtttataa gtgctgtttg ttttttttgg aaatttaggg ttgtttttgg
     2341 ggttgatagc tgaattcgct gagatgtcgt cgatatctaa atcatcatca ccattttcca
     2401 catcttctaa acttttcgct aataaatttt caaatgtatc tgataactgc tcgatatcag
     2461 aactttgaac tggtaccacc tttttttcgt cctcagcgaa aagagaaaaa aaagataaac
     2521 ctaaaattaa aatggttgcc ttgacattca tagaaataca cccctgatat tatatatgtt
     2581 catatattat acggaatcaa tttttttcat agtataattt tttggaaatt attttttttt
     2641 gaggagtttc tatcaataaa atcaacggta atataggcgg cttgaaaagc agccaacttc
     2701 aaaaactgag agccttaggc gatagacgtt taaaagacaa tatattgatt agtatggata
     2761 tagcccgaat catgggtgag atagcaaggg atatcaatcg tcaaatcggc attctgatcg
     2821 atagaacggg gtatattagc catgttatgg tgggtgacta tagctcgata gaaatccctt
     2881 acctagatag aactcgctca accggaaacc gtttacgagg tcttcgcctc attcatactc
     2941 acctaaaaga cgaaaagtta agcgaggagg acttggctga ccttgtttta ctccgactcg
     3001 attatattac agccattatt ccggatgaag tcgggaatcc aaagttcttt tactccgctc
     3061 atgccaatgc agatcctgaa agcgactcac tttgggttgt attagataaa caatacccag
     3121 gccaactcaa aactggattt gcagatgaaa ttcaagacct agaattcaat ctcagtaagt
     3181 tcaaaagtaa tctaaaagaa gcatctggaa aaaacagagc cttcttaatc ggggttcaat
     3241 tccaaaatca gaaataccat agaagtatcg aaagctccat tcatgaacta aaagaacttt
     3301 gtagaacagc agacattcac gtagttgaat ccttctatca aaagaaaaag tccctcgatc
     3361 cagccacagt cattggagtt ggtaaaatca aagagatcct cttaaaggca gtccaaaagg
     3421 aagtagaact tctcattttt gatctagacc taagcccatc ccaagcaaag aagatttccg
     3481 acttctgcga tttaaaagta atcgatcgaa cacaacttat cttggatatt tttgcgcgca
     3541 atgcaaagag tagagatggt aaattacaag tcgaactagc acaattaaaa tatctcaaag
     3601 gtcgactctc agaattagat gataatatgt caagactcac cggaggaatc ggcggaagag
     3661 gacctggaga aacaaaactt gaaattggca aacgaagagt ggaagaaaaa atttctagat
     3721 tagaaaagga attaaaatct cttcgccaaa gaagagagct aaatcgaaag caaagaaaac
     3781 gaaacgagat ccctgttgtt gccatagtgg gttatacaaa tgcaggcaag tccacgctac
     3841 tgaatgtctt aaccaattca gatgtaattg cagaaaataa attatttgca acactcgatc
     3901 caacaactag acgcattcga tttccggaag aaagagaaat tatcttatct gacacagttg
     3961 ggtttattca tgatttgccg ccagatctta caaatgcttt taaagcaacg ttagaggaat
     4021 taggagatgc agatctattg cttcatgtaa ttgatatttc caatccaaac cacaatgagc
     4081 acattcaatc cgtggagaat atactttcct ctcttgaatt actggatgta aaaagaatca
     4141 atgtctataa taaaatagat caaatggaag aagattttaa aactccgaaa gatggaatcg
     4201 gaatctctac actaaaccga atcggcttag aaaaattatt agaaacaatt gagaaggtct
     4261 tatgggaatc atcctaacaa atgacgatgg aattgacgca ccaggaataa aagctctagc
     4321 agatgctgta aatggaaaca aaaaaatcat tgctcccgct actcatcact caggatgcgg
     4381 tcaccaagta acaacacata aacctcttga aatccaagaa agaaacgatt cagaaattgc
     4441 agtctttgga actccagcag attgcagtag gattggaatc actcaaattt ttactaacgc
     4501 agaatgggtt ttatctggaa ttaacgcagg aggaaactta ggagttgatt tatatatttc
     4561 cggaacagcc gccgcagcaa gagaagcttc tattctgggt aaaaaagcaa tcgctatatc
     4621 tcactggatc agagagggaa aaccgattga ttggaaaaga gcaactgccc taaccagtct
     4681 tgttttaaat acgttatttg caagagagct tccgaagaaa cacttttgga atgtaaacct
     4741 tcctcatatt tttgaaggcg agaaagatcc agagttaatt gattgtattc cctccacaga
     4801 tccactacct gttgaattta gaagagaagg agatcgattt atttacacag gggaataccc
     4861 aaatagaaaa agagaagcaa atacggatgt agccgtttgc ttctctggta atattgcgat
     4921 tagtttagtg aaagtctaat tttgactccc agtttgaaat cactctcacc acctgataac
     4981 aaaacgataa taaaagaaag ggtattcaag aattatcatt tctttcatca gtgttaatag
     5041 cagagtcaaa tcgatccctc caattgaggg atctttttta aaatcaaact gagatgaatt
     5101 tatttctttc tacaacggaa gatatactca tccttatctg ctttacctga ttcaattcct
     5161 tccccattac tcatatcaaa tacccatacc atgactttct ttccggaaaa gatactattc
     5221 gattcaaatt cttcgcccgt ccaataattt atgtattgcc aatccttaaa aactccttga
     5281 gatcgataga tttctagtag ttctgccttg cttggaagac gcataccgag agaatcacat
     5341 ttcttttttg cttctaccca agttgctttc ttttgttcgg gtccccaagt ttttgcatct
     5401 aaattgatct tggatttttc atctaacgct ttttggtctg gttcatttgt ttcagtctgc
     5461 tttttttcaa ccactggagg ttcttctact tttttggttt ccactttttg ttcttctacc
     5521 ttagcaggag gagccggatt aacaggtgcc tctgtctttt tttctatctt tggttcagtt
     5581 tttttagttg gttctggctt tggtttttct acttcctttt caggctcttt tacttccgag
     5641 gaaggtattg catttttgct aactggttta aactcatcat actcttttaa ttttttctcc
     5701 atttcttccg gtgagacttg aaccacttct ggttgtaagt ccttgtcaga aattttagct
     5761 gtcttctctg agagtttttc aggagaaagt tttgtatcga tatcatctgc tattttagaa
     5821 gggtcagcat tttttaatac atcaccgagt cctgtttctt ttaagattgt atccgattgt
     5881 ttacggctaa cgctagttgc atttccagtc tgtaaaacca tctctttttc ttttagaaaa
     5941 gaatccaatt tcttgagcgt tgcactcttc gctcttacct caggaggaag attttctact
     6001 tcaggtatta cgactctagt agcgactttt ccatctaaga cggcaacatt tccatttgca
     6061 tctgaaccca tttctacttc atattttgtt ccacgaaccg ccatcactac agtgggagtg
     6121 atcgtctcca ggttgtctct gctatttaac ttatcagaat tcaccattat tttgccagag
     6181 acaattttca tttgaacatt atttgaatct ctttgtgttg cggttgcgag agagaattca
     6241 gaattttctt ttactcgaat cgaaacatta ctttcaattc cagttaactg aagatcacaa
     6301 actgatttag gacctgtctt aattgaatct agatttttaa gaacgtcacc cgactttatt
     6361 tttttatctc ccattctaac atcaccaatc actgtagtaa caagagcctc cggtgcttgt
     6421 ttcttttcat tgcaacttaa aacaataaac agtattagta taaacaatca atagcttttc
     6481 atttttatcc ccttggtttt gtcttacttg agtaaaaccc tgtttctaga ctttggatac
     6541 cacgtggtct gtcaatcgat atcaatggaa ttcacttact tctgaaaata atttatttcg
     6601 aaacgatttt gggaacctct gataaaaaaa aacaaaaaga agaattcctt ctagagaaag
     6661 aaaagccaat caaacctgac aatctaactg gatgtttttt tctggcatca atcgtcagct
     6721 gataaacagg gaaggaagtt ttgattctaa aaatgagctt taggttaaaa tagaactaag
     6781 atttgttgag agagaaatta ccttcctttg aacacaatat aaaccacttg caaggaaaga
     6841 tttttgtatg aatcacagga aaaatacttg actattgaat attgcgctat tattatccga
     6901 ttctcttaat aggttgatat gattttttac ttgtcaatta aatgagatag attccctttt
     6961 aaatacacta tgtctacaca ggctgaatta gaattcaaat actccttctt aaaagatgtt
     7021 cgaaaagaga aggatgctat tttgatgggg attaatccta ccaataaata ccagcacgaa
     7081 ttattaaaat caattaaaac cttaaatacg attcagcatg ctaccaaggg aatgctcttt
     7141 aatatcaata atacgatatt agaacaactc caaattgata tgaagacggc agttacaaac
     7201 cttgcttccc tagtgaacaa tcaactcgcc gcacaagttt gttgctttga atttgatgca
     7261 cacaatcgag aactaaaggt aggctctttt tttattaccc atccgtccga agataaatcc
     7321 tattccatct acaatctttt tgatgaactt ctcagttatt cttataatgc aatcaagtcc
     7381 tattttgata caagagaaca attgttaggc gaaaactttc gaaaagaact tctttggcaa
     7441 atgagttcta agaatgcgga tccaaattca ttcaaaggaa tcagtagtct tttcaatcct
     7501 taccgacgat ttatggaatc aaagaatgga attccgattt cggatgatat gattgaatat
     7561 atcaatcgag aattgaccaa aagacttgta gagtcacaaa ttgcgaaaga ggttcctggt
     7621 catggtttat tgatgctaaa gacaaatgaa atcttagatc atttcgaagc agcagccaat
     7681 gcaatgatcg aaaagttcat acctgccaat gcaaatgatc ctaccctaaa aagcagaatc
     7741 gatcgaatcg ttttagaaga aaaggtttac aatcaagtag aaaattttcc aattaagacc
     7801 gcaagatttg cggcggataa agcgaaagaa attcatcaat acaaaacgat cgctccagga
     7861 aaggcaaata gtatttaccc tggctcactt tgtatacaat ctatcatcag cctagagtca
     7921 gcagcagaag aacgatatgc gattgcatgg agagatgaat gcaataagat gaaacaagaa
     7981 tttaagaaga atattacagt tccttccaat aaatggagta aattaatatt attcgtaaac
     8041 cactctgact ctcttgaaat tcatccagaa gtttggaaag atctaatcaa cgataaagac
     8101 ttattctata ttaaatggca gatgcccaag aatacaatcc atgtgtttac tggaaaagat
     8161 ccaaactttt tcaaagttct agtcacaggt atgctaaatc ttcctgcaaa cgatatgtgg
     8221 aaggcatctg ccttaaaagc actcattgag aaatatgaaa aacaactcag atctctcatg
     8281 acagatcaaa gtttttttca agtatatcaa aatctagaaa aaagaatcta tatgcaatac
     8341 atgccttggt attatagaat ctttttatac tttccatttt cactctttca agatgtaatc
     8401 ttagtaaacg caaagaaaaa aatttctgta gaacaagaag tgttctctag taagaatgat
     8461 gcccacaatt taaaatatat ttcagaaatg caatctaaga aaatggaaaa gtctatcaag
     8521 attaaagagc aggcactttt cgaatctgtc atcgaaacac tcgacctatt ttacttaacg
     8581 atgaaaaaaa tcccaactgt gaatgatgta aaaaattatt ttcctgacat ggaagcattt
     8641 tcaaatatca taaacactcg tagctttaga attctaaatt taccactgaa aggttctgaa
     8701 gaaacagaag tattactcta tcctgacaat aacgattggc aagacaataa aaaagcgtta
     8761 atcgtaaaag ctttagattc cattataaat gatagaaatc ctcacttagt agttgcaaat
     8821 gcagacaaag cgaagattga aaaagcacaa aagcttttaa atgctatcga aggaaacagt
     8881 gctcctaaat taaaagcagc tagctaaatc gatgctccac tttttctgta tggtgaaaga
     8941 gatgatagat agaaaataaa accatttctc gaacagtaag atttcctaag agtggatgag
     9001 gcattttata tttctctaaa tcttcttctt tccaattttc tatttgtttt ttatatttac
     9061 caaaaagttt tgtaaaatct tgaattagct tttgttgaat caccatatcc ggagttgtat
     9121 tctctcgaac aggtgtaaaa attccagctc caccgcccgc atcaatcttt gcgagataag
     9181 cagattttat ttcactcatt gttctagatt tgtctttccc tgaaccaaaa agtaagagag
     9241 caactttagg agaagataaa cccagataaa tcggctgcgt agatttgatt agatggcgaa
     9301 tatttttctc aggagaccaa cctccatcta aaggagattg aaaaaaataa tctactggca
     9361 gatcattata gaatgctgca atcgaattag agattgtttc gatttgattt agaatttcct
     9421 cttttgaata tatagtcttt atatcttcgt aatgtatctt gagcttcata tctaatttct
     9481 ccttgtggct aatcttcgta atcctcataa atcaattgat ttgatcatac taacaaatta
     9541 gcggtttcgt caagtaatac tatctccctg cttctactat tttatcatca atacaaaggg
     9601 caatattttt cattgtctgg actccaatta cacagtttat atgtgaatga taatttgtaa
     9661 aaacttttat gagataatgg tggtaagaat attttttgtt tgacagacgc actacattgt
     9721 agtataatct taaaatgaag aaattcaaat cgaaatggtt tcataaatgg gctaaaaaga
     9781 accatctttc ttgctgattc agttttagta gattccattc aggatttaaa agaaaataag
     9841 agtgtgaata agttaggttt caacttattt aaaataagaa tatcttccgg taataaagga
     9901 aaaaggggat catttcgaac gattgttgtt tatgtatcga atattcgtgc aatatatgtt
     9961 tacggctttt ccaaaaatga gttagagaat atttcaaata aagaattaga agattttaaa
    10021 gaactagcta aaacatacac gaacatgaat gaaatggaaa ttaaattact gttagaacaa
    10081 aattcaattt ttgaattaga ggaataataa tgacaaataa aataaaagaa gccattgccg
    10141 ataatttgca agatattgtc aacgcaggta tagagactac atttacaaaa aagcgattaa
    10201 aagaacttgg tgttaaaatt tctaaaacaa gtattaatcc aaataaaata aagagagtca
    10261 gaaaaaattt aaacgttagt caatctatat ttgctcaatt actaaatgtc agcattgcgt
    10321 cagtcagaca ttgggaacaa ggattaagag agcctacagg ctcaacaatg gttctattgg
    10381 aattgttatc aaaagagcca catatacttg attataggat tacaaagtca aaaagaattg
    10441 cagtttaaat cttagtgcga atcctaaacg ctatgctcaa ataattcatt atacaataga
    10501 gcaaggtgtc ggaacagtaa aaatttctct gggatattag catatctcaa ccacaaaaag
    10561 ttttattcag acagtgctct aaatactaat gttatattga aggctaagtg gactaagttg
    10621 aaaaactata atttgctaat ctgtaataga ctttttcttt gtttgcctct gttaaaaaag
    10681 atcccattcc atgaagatag gtgttataga agatggattt ggattctttt tggatttctg
    10741 tttttggaat aggtattaat gcagcgttat gattttgttt tgctccatcc aaaaaacgta
    10801 aagcatagcc tttgtaatcg gaaggtttat ttattcgttc tccttgttgc cctttttttg
    10861 gtagtcttcg ccaagcctca tctaatttat aatcggtgaa tagttttttt atattttcta
    10921 atgacttact attctgtttt tcgatttgct cttttgtgat tatttcattc gcttttggca
    10981 ttatagaaaa tagaattttt tcataatcca gagtatcatt tatatctaat ttaaaaaaac
    11041 cacgaatcgc taatataaaa aagaaagcaa tgtcttgttc atttaaattt tctttatcaa
    11101 aagtaaaatg agcaaaacaa tttctaatat tatttaaaat ccaaaaggaa gtttcgtaaa
    11161 aagttttttc atcttcatct gagatatctt taaagtgttc cttctgctct gcatctttac
    11221 tttcacctct ttcccattca gagactaaag tcattaaaaa agtatataac ttttgtgttt
    11281 tttctttccc atctaaatga aattcttttt gctgtatacc ttgcagtctt attaaaaatt
    11341 cttttccata ttcctgtgaa agctcgtcta tttttttacc ttctccatct ttctttaaag
    11401 ttctgttcag aagaatttct tcgggagtat tttttagtaa atactcacat ccatctaaga
    11461 ttccccttct gagttggatg taagggttgc tagcctttaa ttggagccat ttgcgaaatg
    11521 atatgtagcc gcgtttgaat ttcggtgaat cttcattctc ttcggatttt tcaaatacgt
    11581 tttctgtttt tagcaaggga atatttgcat ccctacagtg cttttcaaaa tcagaaacag
    11641 agttaccatt tccagtcata aaagcaatct ggtcttcagg aactcccttt cgtataagaa
    11701 ggttccaaat gtaaaatcca gatttttcta aaaaatcttt tctattaaat cctgctggta
    11761 tcagagagtt atttggctca gcgtcagtta aatttatatc aacgataaaa atatcaaagt
    11821 ttaaatattt taggtcatga gtatttatat attcatggta actcttgcaa actactattt
    11881 catgaatgga attttttatt ttaaaataat ttggcaactc tttacttata tcatttagac
    11941 tttcataatc ctcatcaaaa ctttctaatc ctaaaacatc tttaaacaaa tttgctgcta
    12001 gagaagaagg tttatccgat tcttttggaa aatcttcaat ccaaagaatt ctcataaaga
    12061 tctcctttta tatttgttaa cttcaatgtt actttaaatt cagatttttt tgattctcta
    12121 gtaaatcgaa tatctttctg aattttattg attagatatt ctataaattg caaaccgctt
    12181 ttgctatcag aatattttac actttctttg gaaaatcgat ttttacattc aaagaggata
    12241 gttgaatttt cccttttcca agaaatatga attagttctt cgttactgga atatttcaaa
    12301 gcattgaaca tggattcgct gacaacagaa taattcaata aaaaatgaac ccctctttta
    12361 ttgaatttag ttatctcatc tgaaatttct atcttgataa aatctaaatt ttctttcacc
    12421 caagacaata ctagatcagg agataattct tcaaactcta caaaaaggat tttctctaaa
    12481 aaggattttc tcaaacttct taatttttct gaactaaaat tcggaattaa cttttctctt
    12541 tcctgaatca aaccttcgga aaataagaat cgtgaaatat tctgcttaac gcttagtgaa
    12601 aataaatatt taacagactt gtctcctcgt atttctttat gccattcctc cacaaaccta
    12661 ccactatcct gaacgaacaa ttgaaaagta tccaatatat tttctaccat agcaaaagtt
    12721 gaatataatc ctgccagatt gttaaatgct ttgtatgcca gtttatcttc ataatctttt
    12781 tccaatgctt tttttgctag gttcaaggtt tctcgaattg tctcaggtcc attggacaaa
    12841 gagtttctaa aagaatggga aaggtaagaa agaagagttt gtttggtttt aatttctatt
    12901 tcctcttttt ccttttctgc ttgcctggct ttttcttgta gatcataatt gttttgaagt
    12961 aaagaaaatg caaatagctc gcttttaaaa tttcctttat taaatttatt atacaactct
    13021 gtgtataaat ttctaaaatc aaaatttatt aatttatttt tggataatct aacaatgtga
    13081 aatgaaacta aataataaaa attatcacta aaatcttttt ttaaaatttt tctaaataaa
    13141 ttaattaatt ctgcgtgcat tttatttttt tcgtagatgc ttattaagag atcaattgtt
    13201 tttaattcaa cttcttcaaa tacagaccct agtttaatat tatattcatc tagaagatta
    13261 tttatttcat ttttagttaa ctttggccat atttctggaa cgttattttg atattcaaaa
    13321 actaaattat aatgttttaa agataaggtc tctctatttt caatttggta aatttgtcct
    13381 agtataaaat tagctaattt ggatggaaat tttatatttg ataactccaa atagtttatt
    13441 cccaagttat tttgatgagt attgaacgca cagaaaccat aaaaaaagtt taaagtacta
    13501 gatataaaat cttttaattt ttcttctttt aatgatggtt cctttaatag tctatcatgg
    13561 ctctgtaaag cggtatcaaa atcatttaat ataaatttaa gagaagagta aactaaaaga
    13621 attgatttat tgtttggaaa tttatttaat aatgaatcta ttattttaag aaattcattt
    13681 tctttattat tgattattac ataaattact atttgataaa ataaatgaac aacctttgat
    13741 tcagattcgg tatttaaaat aaactggcaa agaccatgat aatttaaatt aaataaattt
    13801 ttaaatttgt ccaaatcgat gcgattttct cctcgcgcta ctactaaagg aaaaataaaa
    13861 ttagaccaaa aaaataaaaa atcattgcca gtagaaattt ctatactctc tgctttttca
    13921 tattgttcat tctggatata ataattaaaa attttaatac atatatcctc attctcttta
    13981 tttaatttta gatatttttg atagtagaga atagctttat tcattttatt tatatcttca
    14041 taagtttctc caatcttttt ccaattttcg taattttcag gttcaaagaa tagctttatt
    14101 ctaagaagtt ctatggctct tgtttctctt ttagatttta ataaaaactt aatttttata
    14161 tttaaatctt tgattattaa tcttttaata tatttattta taactaaaaa aatatttatt
    14221 ttgatgggaa tcttaattag aaaatttgaa atagaatatg aaaatagaag aaaaaaataa
    14281 tataaaatcc atatcaaatc aattggaaat acaataatcc taattaaaaa ttctactttc
    14341 gaatttttct cttccatctt attccaacct caatctattt gaattctcta attttctttg
    14401 atttgctttt ttactgattt cgatcagttc caattttttt ttgcttgtgg acttagaaat
    14461 acttgcttcg tattcatcta acacacctgc atctttggcg gcgctggctt ttatgatttc
    14521 gattaagtct cctacttctt ggataccaat gatcattcct ccagtgacat tggcaagcca
    14581 tgaaaaaaaa ttaggatgac tcaagttgga gacctttttt tgaaagtctc caattcgaat
    14641 tggggctaac gcttgtaaac tctgagagga aaaacccggt ctacaataga ctgaattgat
    14701 tgtaacttgt ttttcatata attcttttgt gatttgaaaa tagctatgtt ggtgagggca
    14761 ttcatgtggt tctttgccac ttgcgtcacc aaatagaaca atagagcgat ttacctttgg
    14821 tcgccaattg atttggaagg catcgtataa cgcatcctct actgcttctt ttgcatctcc
    14881 tccatttccc gttggggttc tctgaataaa ttcttgaatc gtttgaatat cattcgtaat
    14941 cttgcaaact tttgttgtat agggatcacc ttgtccatga tccaaataga aaataattcc
    15001 aatttgtaaa tcaggaatga attgaaataa ttcccgactg agttttataa attcagtttt
    15061 aagtaattgg tgataggttg ccattgatcc tgtcaaatct cccatcaata ctaaatccaa
    15121 actacctttt ttcttatccg aaaattgttc ttttagattt tctgatttct ttttcattaa
    15181 ttgatttatt cccgatttat tagataaatc taatgtaaca atatttgctc ttttagtatt
    15241 aaggttactt atatcatttt ttctgatgga aggtaaattt aaagaattat tactcatatt
    15301 ttttctcctt tttctttttt agaatcatat tctcttctgc tagactcatc actcaacact
    15361 tcataggctt tttgaatctt gaaaaattgt ttcctgtttt cttccttttc ttcttctgat
    15421 agatcaggac tttgagtgtc gggatgaaat ttttttgcta aatctctgta tgcttttttt
    15481 atttcttcga aagtagcgtc aaacgatact ccgaggtctg cataaaaatc actttcttct
    15541 tcagaggttt cttcttttgg ttcccctgat gattcactcc attcatttaa ggattttagg
    15601 aaagtttcta aacttaaaca aaaggtaatt gcttctatca ctgattgaaa cgccattgga
    15661 aagtcccaaa gatcttttga ttcatttgct tttttgaatc taagttctat ttctttgata
    15721 aataaattga gcggaatatg tggattatcc ttagaataag aataaatttc ttctaaatga
    15781 ttttttaatt cttctatttt gttttcaatt tcatctagag gtagtgcaaa tcctttgaag
    15841 gttcctccgc aatatttttc ttctaattct tctgagcgtg aaagcatatc cttacttaac
    15901 ttttgcatct ggcggatcgc ttcctgaact actaacttta cctcttttcc ttctttggat
    15961 ttgtcttttt taaataaatc tattgtttcc tttgtttcct tcaaacttgc aaatttctca
    16021 tcatactcat cgattttctc tgctaatttt ttatttaatt cttctttttc cttaaaaagt
    16081 ttttccaatc cttcttttaa ttctatctct ttttcgttta tcaattcctg tttttcggat
    16141 aactctcttt catgaaaaga aaattcaatt tccttttcgg ctaaaaatgc ttctcgatcg
    16201 gagagattgg tttctttctc aagaatttct gattctttaa aatgaagtcg ttctgtttct
    16261 tgaagaattg atttttctct ttcttctatc tcaaattctt tttctcggat ttgttttaat
    16321 tcttttttta tatctgtctt tttagcagta atccaaattt cagtttgtcg aatttctttt
    16381 gatttctctt ttagctccat ttctttcttt tccaaaaatt ctttctgttg gataacaaga
    16441 aacttctcct tctcaatatt cttaacaaga gcacgaattt cttttagaag ttctatttgc
    16501 gtagaatcaa aactttgatt ggatttatgt atattctgtt ccagttgaaa aatcttatcc
    16561 tgaaaagata atgtatctct cattagagta tgagaattag acaacaactc tctagcgttt
    16621 ttcttttcag agaaaaaggg aattaacgtt ctgaatcgag aaagaatgta tcgacccgaa
    16681 agaattatat ttgtatggaa gaaagaggcg ggctctttct gtaatatgtt attttggctc
    16741 ataacaaggg gagccttttt cactaaaata ttaatgcctt cagaagatca agtatttttt
    16801 ctataaatac acattttaat ttggtgactc tactagcgta ttcttcatct tgcaaatatg
    16861 aattcaaatc gcaaaccaaa caggctcatt gaagaaaaaa gtccttacct attacaacat
    16921 gcttacaatc cagtagattg gtatccctgg tgcgaagaag cttttgaaaa agcaaaaaga
    16981 gaggataaaa ttgtaatcct atcgattggg tatgcgactt gtcactggtg ccatgtgatg
    17041 gaaaaagaat ctttcgaaga cgaaagaacc gcagagatta tgaatcgaga tttcgttgcg
    17101 atcaaagtag atcgagaaga aagacctgat atagataaga tctatatgga tgcgttacat
    17161 gctatggatc aacagggcgg ttggccttta aatatgtttt taactcctga taaaaaacca
    17221 gttgcaggag gaacctattt cccacctgtt caaaagtatg gcagaaaaag tttcacagaa
    17281 attcttgcaa ttatcaaaaa tgtttgggac acaagaaaac aagaactcat tgattcctct
    17341 gaagcaatcg caagctatct acaaaaagaa aacttagcgg ataatccaga tttaactctt
    17401 cctccagaca cagcattttc ttctgcctat caaatgtatg atcgtttttt tgatgatacc
    17461 tattacggat tcaaaaccaa catggtaaat aagtttccac ctagcatggg actttctttt
    17521 ttactttttc actatcatat tacaaaagaa acaaaagctc tccacatggt cgagaaaact
    17581 ttaatggcta tgaagagagg gggaatctat gaccagattg gagggggact ctgtcgatat
    17641 gcgactgatg gaagatggct tgttcctcat tttgaaaaga tgctctatga caattcttta
    17701 tttatagaag ctcttaggga atgctatcaa gtcactcaaa atccatttta taaagaagtg
    17761 gtctatgata ttgtaaaata cattcgaaga gatatgactc taaaagaagg agtgatcgct
    17821 tctgcagaag atgcagacag cgaaggagaa gaaggtaaat tctatctttg gagtctagaa
    17881 gaattcagat ccatttgcaa agaggattct gaagttctag aaaaattttg gaatgtaacc
    17941 gaagaaggaa actttgagga gaaaaatatc ctaaatgaaa ctttcgatat agattttata
    18001 aaagaaaata atttaaacag agaagagttt caatccatcg ttcaacgaaa taaagaaaaa
    18061 cttttagatg tgagagcaaa aagaattcgt cccctccgag acgataaagt tcttacctct
    18121 tggaattgtt tatacctaaa agcgattaca ggcgctgcca ttgcctttgg agacaaatct
    18181 ttattagccg atgcaatcca aatctttgat ttcattcaga aaaatctttt taatgaaaat
    18241 tgtagacttt taagaagata tagaacaggg gaatcgaaaa ttttagccta tctcactgac
    18301 tatgcagaac taacgcttgc ttctatttat ctctacaaag caacttatga tctaaaatac
    18361 attaaaactg ctgcgggact tgcagaagaa acaattcgac tctttcaatc agagaaagga
    18421 gtcttctatg atacaggaat cgatggagaa gcactcattc gaagatcaat agatgcgtat
    18481 gatggagtcg aaccttcagg aaatagtagc atggcacatg catttaactt tttaagttca
    18541 gttggaattg aaacagaacg ttatgagaat atcgccaact ccattttccg tttcttttct
    18601 gagaacttgg ttaaaaatag catcagctat ccatcgatgc tttctgctta ttattatacg
    18661 aaatcctcac cgaaacaaat catcgttttg gcagaaaaag aaaataaaga agcacaagaa
    18721 attctaaact tcttagaaaa agaatacatt ccaaattcaa tcttacttta tgtagaaaaa
    18781 gaaaatctct ctgaggcaga aaaagttcta cccattctaa aagggaagaa ttcagacaaa
    18841 gaagtcgcag tctttatttg taaaaatcag acttgcgatc tacctatcta ttcacttgaa
    18901 gctgtaaaaa aagcgatagg ggtttttacc acggataaac acggatgaac acggatggct
    18961 tttctggttt ataaagcaat tcgatagggg tttggggacg atagtcccca agttgccaca
    19021 cagtctaccg agcgcacgaa gtgcgcgagg tagattactt agctgttgtt tttgctcgtt
    19081 cgattttttc gtaaagcgct ttcgcggttt ctgcaataag ttcttcataa gtgaatgtgg
    19141 aaagcatttt gatttcgtta aaatcgagac caagtgagtc tacaacagaa tacgcaagct
    19201 ctgtatcagc tttataaaaa tgcacacact gacgaatttg aattctagtt gggatatgac
    19261 gcatactttc tactaggttt tcaatcagaa tttctttttt ctcatctgtc atgagtctgt
    19321 atagatttcc tgcttgtgtg tattcatcta gcggataagt cttattaaac gctaaagagt
    19381 catttccgtt cgcactcggt tgcaaggtcc tttcattcat ttcttccaaa ctatttggac
    19441 tataatttac atgagagcca ccgtttgaat cgattcgcat catcccatct ctatatccat
    19501 tttctacata agagcgaggg cgattgattg gaatggtttg gtagtttact ccgagtctat
    19561 gtctttgcgc atctggatag gcaaaaattc tggcttgtaa aagtttatcg ggagaaaacc
    19621 caatccctgg aacgatattt gccggagaaa aggctgcctg ctctacttct gcaaaataat
    19681 tttctggatt tcgatttaat tctaaaatcc ctacttcttt ttcaggagcg atagaatgag
    19741 gccacacttt tgtaacatca aaaggatcaa atgtaaattt ctccaaatct ttgtctgtta
    19801 aaacttgaat aaataatttc cactttggaa aaattcctga ttctatagac tgaaataaat
    19861 cttctgtata atagtctgga ttttctcctg cgattcgaat tgcttcttcc gaagagagag
    19921 atttatttcc ttgctctgat ttaaaatgaa acttcaccca aactttctcg ttctttgcat
    19981 tgataaaact aaatgcatgg caagaaaatc cgtccatata acgatagctt ttcggaattc
    20041 ctctctctga aaaattcatt actacaccgt gtaacgtttc aggagagaga gaccagaaat
    20101 cccacatact cgttgcaata tttaaattgg tttgtggatc tctcttttgc gagtgaatta
    20161 gatctggtaa ttttcgcgca tcgcgtatat aaaaaatagg aatattattt cccgcaaaat
    20221 cccaattccc ctcttctgtg taaaacttaa ttgcaaatcc tcttgggtct cttacagtat
    20281 cggcagttcc tttctcacca cggtaattag aaaagcgaac aaagacagga gtatgttttc
    20341 ccacttccga aaaaatgcgc gccttcgtat agctcgtaat atccgctgta acggtaaaca
    20401 ctccgtatgc ccctgctccc tttgcatgtg taatccgttc tggaattttt tctcggttaa
    20461 aatgtgctaa tttttcgttt aataaatgtg tttctaaaat accatccctt gcatccgatc
    20521 ctatcggcat tcctgaagaa gttgtgtgtc ggttcatttt tttatctccc ctttgtattt
    20581 cgtgagatgc gactactaaa aatttcgatt ttgtctagat tacaagctgt ttttgcttcg
    20641 aataggattg ggattgagag ggattctatc cactttttct attattagga cttataccga
    20701 acgccaaaat taatttccca tttttcaatt acaaataagt gttcgggatt gtgtaaaaac
    20761 aagattttca ccacgaagtg cacgaagacc acgaagagaa attgtaaatt ttcttggaaa
    20821 ttttgacctt cgcgatcttc gtgctcttcg tggttagatt ttttttaagc ttttacacag
    20881 ttcctttacc aaacgctact ccaataaaat ccttctagga agtttctttt ggcgtttggt
    20941 atataaaata caatcgtaaa ctaaataaaa aacacttgct ctctttaagg taaaaaaaca
    21001 tgaatgtgat gtattgaatt cattctttca gatgaattaa aaatcgcaat tgtgagtata
    21061 aaacaaattt agagaaaata aaattagcca atgcggcaaa caaggagtaa cccatgcaat
    21121 ccatttataa aacaatttct attttaagtt taattttcag tcttattgct tgtaatagta
    21181 aaacaaataa cgacaaaacc attttagctg ctggactcct ttttttacaa aatcaaaaaa
    21241 gtaattctgg gattggagga ccgactgcaa ctagggtcta tgctcaatta ggaagtttta
    21301 ctacaaatac aacgaacaag ggaggagttt ctgcaaatag tttctatgca ccaaatggaa
    21361 ttgcattaga ttcaagtggg aatctttatg ttgcagatag tagtaataat agagtgttat
    21421 attacccatc taatagcact acagcaacca gagtctatgg gcctattatg aaacctaacg
    21481 tttattttat ctaatagcac tacagcaacc agagtctatg ggcaaggggg aagttttaca
    21541 acaaataatt cggaaacagg agctgtcagc gcttccagtt tagctgcccc tggtgcagtg
    21601 attgtagatt ccagtggtgg tgtatatatt tcaagtagtg ggcgagttct gtattattca
    21661 tccggtagca caactgcgac cagagtctat gggcaagggg gaaattttac tacaagcaca
    21721 attaacttag gagggataac agcgaatagt ttgtctggtc cagccggttt agctttagat
    21781 tccaataatg ggctctatgt ggcagattac tcaacaagtc gagtcttgta ttatccatct
    21841 ggtagcacaa ctgcaacaag agtttatggg cagttaggta gctttacgaa tagtatagtg
    21901 gatccaagaa atgttactgc ggatactctt tatacaccga caggagtaat tgtggataaa
    21961 agtgataatc tttatgtagc agatacttct aataataggg ttttatacta tccatctgga
    22021 tctattgttc ctactaaggt ttatggtcaa tctgatttca cttctagctc tactctaaat
    22081 acttcaaata gtagtcttgg aggacctaat tttttgacat tagattctaa tggaggactg
    22141 tatgtctcta cgggtgagaa tcgggtagtt tatttcccat ctggcagcac tacagcaacg
    22201 aaagtatatg gacaaggagg aagtttcaca tctagaatcc aaaataatgg tggagtttct
    22261 gcgaatagtt taagcaatcc aacagggatg gctatcgatt ctataggtcg tttatatatt
    22321 gctgattttg gtaatagtag aatactttat tattagatta attagatgtt taaatttttt
    22381 cttatcttaa tatttttcct ttcaatcaat tctatctatt cattagaatt gattctaaaa
    22441 tcaggtgatg cttttatctg tgatccagtg gaagaaaatg atcaatttat taaagttaaa
    22501 aaaaagttag aatcaagatt tagaaatcat caaaggtttc tgcagtcgta taacgtttaa
    22561 aatttcaatt tctttaaaag tttccaatcg tttgtgatac agaaattgaa ttcatttatt
    22621 atgacagaaa ctaaatggat tgagtctgga gttttaaaac tatcaattaa aacataagca
    22681 ttttgtataa tgctgaatat taagaaaagg aacacttcaa agaatgtttt tttaatacca
    22741 attcgtcttc gtgaaaaata tccatagtca atactatttt ataggattga atactttaat
    22801 atctttgata gcagaaaaat catcttcatt tctagttaga acatccaaac taagaatcaa
    22861 tgctgttgcc gctattattg aatcgccgat agaaagtttc ctttgttgtt ttagttcaac
    22921 ggctttatca attatatttc gattcaatgg aataatagta agaatagtaa aagcagattt
    22981 gaaatatatt ttttcttctt ccgttatttt atgatatcct aatacttcca gaatagatat
    23041 ttcagaaata gaattttcgg attgaatata ttccttcaga taggtaaatt catctaaaga
    23101 agaatagatt aaaatatttg aatcaattag tttcattcag ttcttagagg taaatttcta
    23161 tctttcctag tttctctttg ccaaagcata ggatctccga aggaactttt tttaacccct
    23221 tgtgagatga tttttctatg atattctaaa ttgtcttgga ctgaattatc tttatccaat
    23281 atttttattc caaccctctt tgcaaaactg agaattaaaa ataaatcctg ttcattttca
    23341 atatctaatg ttagtttcat agtttctagt gcctatcggc gacttgggaa gttccccaaa
    23401 ccccttttct tttatatcct tgtataaatt aattctgtat ttcaaaactt ggcaagtctt
    23461 tttttaactt ccatctttct atctaaattt agaaagcatg ctaagtatat aatcctttta
    23521 aagcactaaa taaaaatttc taaccaagaa tcccttccta ataaaaattc tataaagaaa
    23581 agactgcacc tatccaatga aactccattg cccaaatgct atcataacaa ataaaatttt
    23641 caatgaatac aacccaaaat atacgttagt tctaaaatat aaatattata atttaaatcc
    23701 ttatcgttct tttctcggtg ttcaatcggt ggtaatcagt tttcttttac ttgtgtgttt
    23761 tcacatttaa aacaacaagt ttataaagtg agttaataaa attttaattc tttgatcagt
    23821 ttagaaatgt gtataaaatc tttatcattg tgtagtaagt gtaatttatt ttcaatgcat
    23881 gtctgaacaa tcaaacaatc gatagtggag cgaactgtaa ttccagactt tctacactta
    23941 taataaatct cagcagcttt tttataggaa tccaagtttt tcaattcata aaatttttga
    24001 gtttctaagt attcgtataa agttttaaat tctttttcgg aagaggcacc ttgtaatact
    24061 tccatgtata tcattgaatt gattccaaat gggattttgc tttttagaac ttcatcaaat
    24121 tttatgacgt gcgctccaat attaccagaa aagtaatcta gaagcactga agtatccact
    24181 aatatcattt tccagctctt aaacttttat aatcataacc tttcttaatt tgaatttttc
    24241 ctttcaaatc tcttaaatcc ttctttttcc tattttctac aaattcaagc agagcctgat
    24301 gaatgacttc tttttttgtt tttaaaccag aataaagaaa tgccacttcc atcaattcat
    24361 catccaaaac aatattcgtt ctcattcata caccttccta tacacatacc aaagcacatt
    24421 ttaagcaata gtcaatcgtt tttctataaa tagaacactc tgtaaggtga attactcaat
    24481 ttctttttcg atgttttttc ggtagagaat tcgttcatcc caaggagtat aacgtccaac
    24541 tagacccgga cgacataggg caatgtcatt tgcaaaatga cggaattcta cttcattgat
    24601 ttccccatct ggttttagaa ttttttttac ttcttcgtca atgagtcgaa tataaatatc
    24661 ataccacatc acatgactga tcatgagcca tagcacataa tactgattta cctcatcaaa
    24721 gtataattta ttgtattcat catcgtaaat tagtttcaga ttctcctttt ccatcattgt
    24781 tttggtcgct ccacacgctt gaattcaaat ggtttaatcg ttttctttgt gatttcttcc
    24841 ttcatagatt cttttgtagg agagtgtctt ggatctactt tgaattcctg taggtgaggc
    24901 aatacaactg gatttcttga gagagttaaa cttcccatat aacgatcata aacttctact
    24961 gaaattaaac tcatcaactc atttacattg atgataattt ggtttccccc acaagcatgg
    25021 gcaacttctg taaaaatttc atagatttct gcttttcgat ttttccaatc ggtgtcgttc
    25081 aggcgaatcg gtgttatccc cggaggagga tttactaaaa atccatccat tatcttctca
    25141 cttcccgata agcctttgta ttcaactctt ctatcttcgt ttatatcgaa aataatttgc
    25201 gtataatcta tttttaattt cttcatttta tttccagtaa gaacagcatc tgttttatct
    25261 gttggctcac catccgtaaa taaaatgact ttattgattg tatccggatt tctatgatgc
    25321 aaaactttct tcataaaact tccataatgt gttccttttc tttcaatctc ctttccactg
    25381 agtggaaatt ttgcagggat acaggtttcg gaaaatacat agagttgaag ttttacattc
    25441 agcatacatt cagaaatatg agtgtagaat aaattcacag cagatatgac tttatctagt
    25501 ttatccatcg tattcatact ataggaataa tctactacta gatgaagcat cacttctaca
    25561 tcttcaggaa gacgaaattt atctttgagt ctacccgata aaatatcagc aagagtgatc
    25621 ttttcttcta gatgagaaaa catagaagga ggattggaac ttgcgatttt tatttcaaaa
    25681 gaattgtaag tatctttaat cagatgatct tggttaacat aggaagtaga ggttaataga
    25741 gtattagccg gtaaaatcag tccctgattt tctattaagt tttttttaat tctctgaatt
    25801 tcatctctaa aatcgttaaa ttgtttgagt cttttttcta tggttctaaa acccatagac
    25861 attctagaag cgctttcccc tttttgcacc tcagcaacaa tgacttctct atttggtaaa
    25921 aagtgatatt ttgtcgttag ttcccgaatg agcgtaatat ttaaagctgg aaaatgtgct
    25981 agttccaaag gtaaaccgtt gttaacctta actcccccca ctacgatcat acccgcttct
    26041 aaatctttcc ataaaacttt ccgcaggttt ttaaatccgg gaatttctag catattgttg
    26101 aatccttttt cttagataga tttgacaaat cccatttcgt aaactcgaag agtataaaat
    26161 aacaaaaaac aaaataaagc aagtatttta tttggtctgt tataaagtcc gaataggaca
    26221 agattcaagg tgagttagtt aatttcttct tctgtggcgt agattgtaaa aaaacgatcc
    26281 atcgatgcta attgtaatac agacttgatt tcataagata tagaaagaag agcaaatttt
    26341 ccttctctct ccaacattct ctttcttaga tttgcaatga gtgcaattcc agaagaatca
    26401 aagtaattta ccttactcat atcaaaaata aaagatttta cttcttcttc ttctattttt
    26461 tcgagggctt gtttcttaat aattggtgag gtataaatat ccagatttcc agaaaattgt
    26521 aatatcttat tatctccctt atttctaaat gctatttcca tctgttaatc cgtaattaca
    26581 tgtttttcca cactattttt taaaagcaag agtaatttta ttgaactagg attccgttga
    26641 agaagaattc aaaatgacat aatttaaaat acttcgatct cgaaacgtta gagtaatcta
    26701 ttctagtgaa caacatataa tttttttata tcgactgaaa agaattctta tttctttact
    26761 actctattca ttctcaactt atttagtcaa tgtatagaaa acataagttc aaacccccag
    26821 ctccattaat aaaacaatta ggaattatat ttttttattt ttcataaata aaattgaaat
    26881 tggaaaatta tagagagttt ttcaaagttg caaatacccc cataaaatga aaataagagg
    26941 atcagaatga atctaaaatc tagcaaacta atcaccctat ttttgagtct ttgctctctt
    27001 tcactttctg cacaagtgac agttggacaa ttatttcaaa aaccagcagt aataggtgac
    27061 tccctaagcc aaggcttcta tggagtaacc gtcgaaagga aaacacaaga ctgggcttat
    27121 ccagttttag tgagtaaaca agcaggatct agtgtttcat ataacgtttt aaaaggacct
    27181 tatgcgaacc tagaagatat tttaaaatta aattgcggtc ctatctgcct agcaagtgct
    27241 attataggtg gaaatgaaag cacagtcgca cttccaacac atgcaggaat cactggtgcc
    27301 gattacacca atgcacttta cacaagcggt agttgtgaag atattagagc aaccaaactg
    27361 gtaaaagaat ggtattggga aacttggtat tggtatacat accgctgggt aactgctcct
    27421 gattgtgtta ctccagataa attccaccaa tatggattaa gaggttcagg aacacaaatt
    27481 caaattatgg aaaaggttaa acccactttt ctttttggaa ctgttgcggc taaccacgta
    27541 ttatgcacgg ctcttgcaac aaacctagca tgtttagatt atcctagatt tgaaaaagac
    27601 attcgtgaat caatgagaag actagcagca atgggaagtt tgaaaggggg agtcttattc
    27661 acagtgccta acgttactgc aattggatat ttagaacaat atactgatcc acaaaacaga
    27721 ccaggttttt ctggacgtaa agcattttac agaagttctg tctcaagtgc agatcaagtt
    27781 ttagatgaaa cagaaatagc taccattact tctttcttaa caaaattaaa caacgaagta
    27841 aaaacacaag ctacctctat gggttttgct ttagctgatg caaaagtttt atttgatgat
    27901 atcaaagaaa atggacggcc aatcaaaagc ccaagtggat acagccctgg ttcagcaaaa
    27961 gcaaattggc ctcttccaaa tcaacctggt ttgtttggac tagatggagt gcatccaaat
    28021 atgtatggtc atgctgttat ggcaaacgaa ctaatcaaag cgatcaattc aaagtatgga
    28081 atatccattc ctcaagtttc tgaatacaat gcttggtatt atgatgaatt gaatagaaac
    28141 ccagttgatc taaagaattt cttaaccaat aatatttttg gtcaagcaat ctcttggctc
    28201 ataggcatat ttgcataaaa ttagaggtta atacatattt accacaagga gtaatttatg
    28261 aataaaaaat ttcttataat aggtgggatt gcactagcaa ttctcgcaat cgttgtttta
    28321 tttaagccaa ctactggaaa aaagaaaaca gctcaaacca ataacgtatc tgactcagat
    28381 aacgtttcct catcaagcgg gttttctaat ttttcagatg ctccaagtcc ttctactgag
    28441 aatccatctt tagaggcaga agcagaaaaa ctttggccac atttagcaaa acaagataac
    28501 tctgctaaga agaaagaaag agttagagaa gaatggtctg catttgcaaa caaatatcca
    28561 aaaaactttt atattccagc tgaaatgaaa gctcctatga cagaagcaga aatggctgaa
    28621 aaaagaaaag aagtggatat tgtttccaaa attgaatcca aaattgctgt agtggaagca
    28681 aatgcaagaa aaacagaagc aggaagcact ccaccgaaag cacctagcaa gccagatgtg
    28741 accccagaag aacaaagaat ctacattgga tttaaaacaa aagagttgga atcaagaatt
    28801 caactcattg aatattccat tgataattct ggaatcagcg gagatcaatt gactgtagct
    28861 agaaaagata tcgctggctg gaaaaaagag ttaagcgatc tacaagatgt agaaaaaaag
    28921 attcctagca cctaatagac tcaaatggat tgtgtaaaat cttaaacatg gtttgcaaac
    28981 cctaagcact gactccaaaa gtgctttgaa gtttttacac agtcctattt ttattatttt
    29041 tcatatccaa atacttagga tacagacaca ttcatgttaa ttaaattact atttctattt
    29101 ctaatttcac tcatctcttg taaaggtccc gataaaccag atacacttcc cacaataaaa
    29161 gagtcgccta tgaaaacaaa agaggaaata aaaagagatt taaaatctac caacttctat
    29221 gaaagatcac aagcacttgt tgagatcggc aaactcaaag agaaagaatt cataccagag
    29281 attaaacaac ttcttaaaaa agatccaaat cctgccgttc gaggtagcgc agctctttgc
    29341 cttgcagaat tagaagataa atcctctact ttagacatta tagatctttt caaagataaa
    29401 gatatttcta ctgacgtcgt tcttgatgct ctcacaagaa tgaaagatcc ccaagccatt
    29461 ccctctataa ttccagttct agattcaagt gatcatactc atagattatt agcagtagaa
    29521 gctcttaccc aaattggatc cacaaatgga accgataaaa tcatcacaat ggcaagtgcg
    29581 aataaagata ttgaaaaagc aaaaacttat gcaatggtaa ttggaaaatt aaaactagta
    29641 aaaggtgaaa atatattatt acaattagca gataccacag aagcttctcc taccctagct
    29701 gccacctacc tagcacttgg tagaatcaaa agtaaaaaat cagttcctat attagcgaaa
    29761 gcaattggaa aagattttga taaaggaaga gaaaattcca tcttatctct aaaagaaatt
    29821 ggggatacag cggcaaatcc tttgctaata gattatttaa aaaataaaaa taaagaaata
    29881 caaatgggtg cggcagacgt aatctcaaca ctctatgatt taaaaacagc agaaaatgtt
    29941 gcaaagttat tagaatctag cgataaatca gtgtttgccc ccagtgctta tattttaggt
    30001 agatataaat atgaaccttc tagaaaaaaa attgaagcta tagcaaccga caaatcaaat
    30061 cctgatagag aaatcataac acaatctcta ggctggcttc agaacaaaga aagtattcct
    30121 gttctaaaac aaatcctgca agaaaaagaa ggagaaggta gatacggtgc tgcttggtct
    30181 ttagggatgc tcggagcaga agatgcaatt gatcttttag aggaagctgc gaatagtaga
    30241 gataataaat tagctcgtat tgcaattgaa agtctaggtc aaatacagtc gcctaagagt
    30301 ctatcgtttc tttccaaaaa aatagaaaca aacaaagaac tttcttccag tatattagct
    30361 tccattgctt ctattccagg acaagaggca acaaaggttc tagataaata tgctctctct
    30421 acagacagtg tcatccaacg ttctgcctta caagctattt tacaaagaaa agacaaagaa
    30481 aatattccag tattgattaa aatcttagaa gagaataaag gtgcggacac agctagcttt
    30541 gcccagttag cacttcgagg gattactgga gaaaattttt cgtctagaaa tgaatggatc
    30601 aattggtttt ataaaaaaaa ataggaggat aaaatggatt ttgataaagc aattgcaaca
    30661 catgtagaat ggaaggcaaa actaagatcc tatttaaaaa acctagattc taatgtagat
    30721 ccaaagatcg ttgaaaaaga taccgaatgt tctctcggga aatggatcta cggagaaggt
    30781 gcaaagtata aagataatga atactatctt caattggtat ctgaacatgc cagatttcat
    30841 atctgtgcag cagctgtaat ccgagaaata attgcaggca atatttccaa agcagaatcc
    30901 atgattggac ttacaagcga atacactgaa gtttctgctt ctattgtaac taagatcaga
    30961 aaaatgcgag accagataga aaaataaaac ttttccttga ctactattga ttcgaaagca
    31021 aagtttagac aaagtatagt cttatgttta gaatcttaat ttattgctta ttttttgttt
    31081 ttcctgtgtt ttccgataac ttaaatgttt ccttaacaca aaaatgcgag caaatggatt
    31141 gtaaaccaag aacttggtat gcaatagata gttttgatcc ctccttttta actctctcag
    31201 aaatagataa atcttggaaa gagatcactc aatttcctat ctggttaaat aaatttttta
    31261 caaaagatgg aagtcttgct acctattctt tagcaactaa ctttgattta ccgcaagaac
    31321 tactcagtca caaggaacaa ataggaatcc gttttggaga aatcggggaa gtatttgaaa
    31381 tctacataaa cggaaatcta attgcgaaag aaggaatcat tgagaatgac aaggtttcat
    31441 ttcatagaac tgtaagaggt aaggtttggc aagttcccaa ggaatattta aaagaaacga
    31501 ataacattgt tttagtaaaa atttctggtc atcccaaatt tgatcatact ggtttttatc
    31561 ttacaaaata ctatgatatc ggtttggctg atgctctaaa atacgatgaa caagatagaa
    31621 taagtctagc tctgatcggt atttacttcg tcgttggact ttaccatatc tttttatatt
    31681 tcagaagaag agtagagaat tacaacttct attatggagc ctttgcggtt atcgtaagcc
    31741 tctatatgta tacacgaaca tcagctatct ttgaatttga ttgggacact gcactcattc
    31801 aaagattcga atttgcaata ttatttcctg gtgttgcttt ttttgtaaag tcactggatt
    31861 cacttttatt aaataacgta aacaagtggt cgaggtatta tggctacttc tcctactctt
    31921 tgtgtgggtt aacactcgtt accccagaaa tgtatctagc cgaatatatt ttacgtttat
    31981 ggcaagtttc cgttctactt tgtgttcttc ctctattaat tcgaattttc tatattgcaa
    32041 ttaaaaataa agtaagcaat gcaaaaaggc tatttattgg aatggttgtt tttgtattgt
    32101 cggcaatcta tgatatcgtt gactctgcat ttttaaatac aggtctagct ttttcaaaat
    32161 atacttttat gatgtatatt ctgggttttg ccgcagtgct tgcagatcgg tttatatcaa
    32221 ttcatattga aatcgaagaa ctaaatgaaa acctagaaaa aaaagtagag ctgagaacac
    32281 aagaactctc gacgagttta gatcaaatta aaaatttaaa agatcaacaa gatggagatt
    32341 atttcctaac gtctctgctt ttacatccct tgggagcaaa ccaagcaaga tcacaggaag
    32401 tgaaagtaga gtttttagta aaacaaaaga agaagttcca gttcaaagaa tggaaatccg
    32461 agataggtgg agatttgtgt cggtcgcaaa ctctatattt taaaaataga cctgttacag
    32521 tttttctaaa tgcagatgca atgggtaaat cgatgcaagg tgcgggcggt gcactcgttg
    32581 caggtgctgt atttaattct attctggaaa gaaccatgct tagctctaca gttcaaaatc
    32641 aatatccaga aaaatggctc aaaaattcct ttgttgaaat gcacaaagtt ttcgaaagct
    32701 ttgatggaag tatgctaatc tcagtagtta tgggagtcat cgacaatacc acaggtctac
    32761 tttattttat taacgcagaa cacccctata ctgttttgta tagagatggc gagacctctt
    32821 ttattggaca tgaaaaattg tatcgtaagc ttggatctag tatggtggat gattttatat
    32881 cgattcaaac ttttcaatta aaacatgggg acgttattat aaatggatca gatggtagag
    32941 atgatctcat gatagaagat aagacagaaa ataaaaaaat actaaattat gatgaaaaaa
    33001 tttttctatc tgttgtaaaa aaaggagaag gtgagatcaa taaaatctat gataagcttg
    33061 tagagacagg tgagtttaca gatgacttat ctctagttcg aattgaatat agaggaaata
    33121 acttagcgat tgaatctttt gatgaaacag aaatacctga tgtggaagta tcctttaaag
    33181 caaataccag ggaagcttta gagtcgatag acgcacactt agcagagttc aaaaatagtg
    33241 actccaaaaa tttacgagcg ctcaaatata taattgtagg ttatatccgt ctgaaaaaat
    33301 ttaaaacagc tatcgattta gcatttgaat atttagaaat aattccagcg gatacggaag
    33361 tgatatatta tttgtcttat tctttccgta aagataagca atttcttcaa gcaatagaag
    33421 aaggagagcg ggtaagaatt cgtaaccctc atcactctaa aaaccttatc aacctagcaa
    33481 gaagttatgc atcactcggt aacttctctc gagcaaaaga aatcatatta cttgccattc
    33541 aaatcaatcc agaagattta aaatcgattc gtctacaaat gcaaatcgaa aaagcaatcc
    33601 gcgcacaaag ttaagagaaa cttcctaaga ttaccaccga tacgcaccga ttatcaccga
    33661 gggacaatgc tctcaactta agagaaatca aaaagaatta accaccaaga aaagagaaga
    33721 tttattattt attcacttcg tgagtcttcg tggttaaatt tttttacatc gatctcgcat
    33781 taactaaata ttttctatac tctaatctaa aaaatttcca aaaaaaatag aaggtaatgg
    33841 gaagaattgc taaataaact aaaacccaaa gattccactc actagaatag aaatgatagt
    33901 tttctaaatg aagaagcagc aaattgtgac taacaatgat tacactccac accgtaaata
    33961 ctcttgcaaa aagatatcca caaaaattca atattacttt tcgattgtaa ggaacacgaa
    34021 aatatatttt gtaaaaagag tttttttcat tgtataaaaa gaaggatata aactccttca
    34081 gtgtgtaatt tttttctttg ggataataaa tatgaacaga gttgttaaac gaataatata
    34141 gaagcaataa agtaaaaaaa acaacaaaag cattcacaga taatagagtt gcccaacctt
    34201 gatttccaac tgacacaagg ggatttaatt caaattttag atctggagta atcaaataag
    34261 tcaaataagc atctagactt cgaacaaata tcatagaaac aactagaaac caaaaattca
    34321 tcccaccctt ccttacggat tacagtagtt atactctatt acaaaaagta aatttttaat
    34381 ttattcaatt caaaaatcgc tagtgtgaaa ttgaattcaa aataatttga aatcaatttc
    34441 attagattca atataatttt cataggagtt atctatgaca ctgagtaatc catttaataa
    34501 gtctgaatta aaatcggcaa tacaggattt aaaacaaaat cttgcaaact cctttcaagc
    34561 aatcgatgag aaaaccttct ttcaaaaacc agagaaaggc tggtcaccgg ctgaaaatat
    34621 tcagcatatg aatcgtgtca ctaaggttct aggacttaca tatgttacac ctaaattcat
    34681 agcatcgttt atttttggag aaacgaatca taaatctaaa acgatgaacg aaatggcaac
    34741 tatctatctg gatgcactta gacaagggaa agattcagga ttttttgccg caaaaacgga
    34801 aaaaacagat ataaatacta caaagcgaaa agaagaatta attcgagatt ggaactctga
    34861 gtgggaaaaa tataaaacag ccattgatgg ttggagtgaa gaagatctag ataaaattct
    34921 aatgccccat cccttcttag gaaaaatcac tgcgagagaa atgtatatgg ttgggatctt
    34981 gcatccagtt caccatgcaa gtattgttgc gaatagatta ggagttaatt ggaattattt
    35041 ttaattctta ttttttatat atgagtgaaa ttgtagtttt attatcactt gctgttgtcc
    35101 agacattctt ctctatttta tttattatca gtaaaaaaaa caaatcaacc cattctatca
    35161 tcgcatctct ttggataata ctcatttgcc tcgcagcttt aagacgggga ctttctattt
    35221 tatttccaga tgaatctaat tttttccaaa aagtgatatt ctacccactt gcctatggtc
    35281 cttgtcttta tctttatacc agaatgcaaa ctaaaaatga aaagacattt ttcaaaaaag
    35341 acttacttca ctttttcccc tttctttttt ttctatttct aacttttatt tctatgattc
    35401 gaatgcaaaa tggctctatt tattttgaac ggagtaaaat attttctgtc attgagggca
    35461 tttttgcaat cgctgcttgt atttccgttt cagtctataa cattctatcc tatcgcatga
    35521 ttaaagaaca tatacttcag atacaagata aattctcgta tctatcgatt gataatacac
    35581 tttcttggat ttctaggttt tcaatcctat tcttaatttt tttgattaat caattggtcg
    35641 tatttttgta ttctactttc tatgggaaag tattactgaa tcctgtggct aatgtttatt
    35701 tgctactcgg ttttttatat acactcagtt tcttttttat gagacaagat gatgtctata
    35761 atcaaaccaa aaatttcgaa gatataatta aaaaagaaaa gtatagcaag tctggtctat
    35821 ctaaaacaaa aatggaacaa ctagaaaata tacttttaga ttatatagaa aaggaaaaac
    35881 cttatttaaa tccagaccta gtaatctctg acattgcaag tggaatcggt attagcacca
    35941 atcatttaag tcaaatcatc aatactaaat ttgaaaaaaa tttttttatg tttatcaatg
    36001 attttcgcat tgcagaggtt ataaaaaaaa tgaatgattc gaattacgat gagtatccgg
    36061 taattcgaat tgcttatgat tgtggattca attcaaaatc ttcttttaat tccattttta
    36121 gaaaagcaac gggcatgaca cctgctcaat ttagatcaaa gagaaacaga gaatcaaata
    36181 ctctttgatt ttctttgatc tgtttgaact aactattttt ttttgaaggt tagtttgatg
    36241 taaggaaaat cactatactt tttttaataa aatcaaatcc tttcaaaagg agtatttgaa
    36301 tggatgttgc aagtcttgat ttatggtttc gtcgtgggat aagtgtaatc acactttttt
    36361 ttggaatcgt tcaactttat ttatcagaaa aaaatatttg gatcgatgca ggtttcatta
    36421 tatgtatggc cgggatcgta ggatattata caaactttct tgcaattaag atgctctttc
    36481 aacctaaaca aggaaaagtt ttaggatggg aaggactcgt tcccaaaaac aaagcaaata
    36541 tagcaaaaag tttaggtcaa agtattcaaa ccaatctact cgctcctgaa atcattttag
    36601 aatatgttta cgaaagaaaa ctcatcgaaa caggaaccca aaaagtagga gagtggttag
    36661 acgaactttt gaaagaccaa gtatttcgtg ataaagttac agaaaaaata atctctttat
    36721 tacaagaaaa aggtcccgaa ttactctctc aaatttttaa cttcacagaa gaaacgttaa
    36781 aggacatagc tcgaaatcca gaagaaatca aaaaagtttg ggaacagatt cgatttagaa
    36841 tcaatgaata tttgcaatcg agacaaaata gagaatattt agtaacacaa acaaggaaaa
    36901 ttattttaga agaattacca agattgtctg tggttttaaa caatgcaatc gatgaatatt
    36961 taaaacaaaa agatacgata ggcaatattg gatttgggat caaaaagatt atatcttttg
    37021 acaatgcagc attacaagac ttacttcaaa aattcattga agatgaggat acaacaaatc
    37081 aattgatggg agtcatggat atactcgttt tagatttaca aaagaaaatg gaagcacaag
    37141 agactcagga ttatattatt actcaagtaa aagactggtt tgatgttgcg agtgagtatt
    37201 ctagaaaaac atttttgcca tccgcaatcg agagattgaa gaaatattta gatgatccag
    37261 acaattggga taaggtagga aattattctt ttaaagttct agattatgca aaagataagt
    37321 cacttgagtt tatgcaatcg caagaaggca gtgaatattt aaaaacaaat atcgcaaaaa
    37381 tcattcacca agtcaacgtt acaaatcttg tagaagagca agtaatgaaa ttagatacgg
    37441 atgaattaga aaaaatgatt ctagataata ccggtggaaa tttagtaatg atacaatttt
    37501 taggtggagt gcttggactc attgctggat tcatacaagt ccatatttat ttttccgttc
    37561 ctgttctagg tcttgtagtt ctcacttgga tttcctacta tcgtaatcag aaaaaatata
    37621 atattcagac taagtgattt cgatttttaa attgcattca ttttcaaaac aagaatggta
    37681 ttaaaacttt tttatacaga ttgactcttt tttgctatga gttgaatagg tattttacaa
    37741 gatacactag ttcaataaat aggatcgtgt tattaaaaaa attttatact tagggggaaa
    37801 taaatgaaag gcagaatcta tccaattttt ttgattcttt tctttttagt tcactgtgat
    37861 ggaattcgga aatttctaat tcaaaaatta ggagcagcag ataaatataa agcagaagga
    37921 aatatagcaa acctatcacc tatattttcc ggtcaggatg aaaaaaggcg gaaaatcgat
    37981 attaccctcc gcgaagtggc ttccgggttt attcaaccaa ccgatatgca attccctcca
    38041 ggagagtcag aggaattttt tattttagaa aagcagggaa gaattctttg gtccaaggta
    38101 aacgggaaag agaataaaga aattttaagt ataaaagtaa aagcggaatc agaagaagga
    38161 ctactcggtc tcgcctttca tcccgaattt agaaaaaatg gaaaaatata tgtaaactat
    38221 gtagctggtg taggtaaaaa agatattagc cgtatctctg aatttacatc tacaaatccg
    38281 aaagatttac aaaactcgaa actagagtca gaaagaatta tcatggaagt agagcaacct
    38341 tatgaaaatc acaatgcagg acaaattctt ttcggtaaag acaatatgct ttatataggc
    38401 tggggtgacg gtgggtggaa agatgatcca agaaaacaag gacagaatcc aaaaactttt
    38461 ttaggcagta tgcttcgtat tgatgtagat tccactcctg acaaagacaa agcctataaa
    38521 gttccagaag acaatccttt ttttacggat aaatgctgta agccggaaac attcgcgtat
    38581 ggatttagaa atccttggag atttagcttt gatcccaaag gtagaatgat tttagccgat
    38641 gttggacaag atctctggga agaagtggat atagttgaaa aaggaaaaaa ttatgggtgg
    38701 aacatacgag aaggatctca ttgttttgaa ccaaaagaaa attgcaaaac agaaaatctt
    38761 gcagatccaa tttatgatta tgggagagat gatggtcaat ccattacagg cggttatgtt
    38821 tatacaggaa agaatatacc tagcttgagc ggcaaatacg tgtttgccga ttttgtatca
    38881 gggagagtat gggcattaga tcttccggag aatccaacag aaagagtaaa agaagcttat
    38941 acacttggaa aatggcctgt tctcatatcc tcatttgcaa aagacggcaa tggagatgtt
    39001 tatctcttag atttagggaa aggaaaagtt tttaaaatag aaccaaaatg aaacaagaat
    39061 tatttaaaca agcaattgta gaatttgata aagcaaattc acttgatcca aatcttataa
    39121 cgataggcga tgaacaaaag ccaagagaac tcgcctattc tgaatggctc tccgactggg
    39181 tgcaaagatt aaacccaaat gcctcagaac ctctccagtt ggcagctcgc tgccaacatt
    39241 taaagcgatg ggagattccg cgttctaagt atcctgatgg attaaaagga tatacaaagt
    39301 ggaaaaaaga actagcagaa tttcacgcac aggaaggaga gaaaattctt aaaaaaattg
    39361 ggtatgatcc agctactata gaaagagtaa aagagataaa tctaaagaag aatctaaagt
    39421 ctgactctga agtgcaaacc attgaagatg cactttgctt agtcacactt gcttatcaaa
    39481 tagaggattt cagcaaaaaa cactccgatg aaaaaatgat tgagatcatt cgaaagactt
    39541 ggggaaaaat gagcaacaaa gcaagagagg aagcattaaa attaaagtat tcagaaagag
    39601 tgctttcctt gatacagaaa gcgatttagg caaaaaaaaa ccgaggtgtc tccacctcgg
    39661 atcaatagtt tctaaaaaga aagcctgcta agcttacgct acagcctcag cggattctaa
    39721 ctcctcctct aaagccatat tcctgcgcga acccttttca attttttcag aatgatttcc
    39781 ttttccgttt gaagtttctt tctcacttac tacaccagcg gttttattgc tgggaagatc
    39841 atttagatct cgaatctgtg cttctaattt atctagtatt tgtggatttt cgagtaggta
    39901 gtttttaaca gtttccttac cttgcccaat cttctcacca ttatatgaat accaagatcc
    39961 accttttact aaaatttcat gttttactgc aagatcaata atggaacttt ctctgctaat
    40021 tccagaatta aacataatat caaactcagc ttgtttaaat ggaggagcgc atttattttt
    40081 cacaactttg acgcggactc tgtttccaat tggatcttct ttgtctttga gtgtttctat
    40141 cttacgaatg tccatacgaa cactcgcata aaacttgaga gcatttccgc ctgttgtagt
    40201 ttcaggagag ccaaacatta caccaatctt catacgaatc tggttaataa aaacaacggt
    40261 tgttttagat ttagatatag ttccagttaa ttttcggaga gcctgagaca ttagacgtgc
    40321 ttgtaaaccc atgtgagaat ctcccatatc tccttcaatc tctgctttag gaactagggc
    40381 tgcaacagaa tcaattacaa taatatcaat cgcattgcta cgaacaagag attcgcaaat
    40441 ttcgagcgcc tcttctccat tgtctggttg agatacaagt aaatcgtcta tatttacacc
    40501 gagcttctta gcataggatg gatcgagtgc atgttctgca tcaataaatg cagcaatacc
    40561 gccttttttc tgagcttgtg cgattgtgga aagcatcaat gtagtttttc cagaagattc
    40621 aggaccgtaa atttctacaa ttctacctac aggtagtccc ccaattccaa gtgctatatc
    40681 tagatctaaa gatccagttg atataacagg aattgcaaca tcggattgtg cacctaggcg
    40741 catgatagag cctttgccaa attgtttttc aatttgaccc atagcagcat caattgcacg
    40801 ttttctctct tcgtttttct cagagagtat ttcttctgat ttatcctctt ttcctttttt
    40861 catgaataac tcctatttgg tttcttatat aattgggtaa aaaaagttta atttgcgcgg
    40921 tatttttgaa ttttttttta aaaattctca aaaaaacaat ttttaccaga atctattctg
    40981 attatgcatc cctgcatgga caaaatagag gataatttgt aattatttcc acttttccaa
    41041 tattcttaga aatactgagc tatgtataga tttttttttg agaaaaaagg tttttttttc
    41101 gatttttaag gcattttttt agcaatggtt ggcacagttt atgaaaaatt caagatttca
    41161 accagctttc aaaactagag atatacaact tttatctatt ttgaaatttc atttagagga
    41221 agaagaaatt ccatacttcg taacaggtga aaattttctt tttctagaaa atctagcagt
    41281 tccttctcat gaatcgtatg cagttttata tcttcttcca gaagattttg aaaaatttga
    41341 gaaattatta gaagatggat tcgtttaaat ttcttttgag cgatttctta tcgaacacag
    41401 cgaacagaaa tagatttatt tttcgctaag ctaaattttg ctccgtttaa aagtgtaaaa
    41461 tagtaggctc tgtctgatga gatttctgaa gcagtccaat aagtattccc atcttttttc
    41521 catgcagaca attcttcttt atcaaaacta gtttttaatt cctcaagaga aggaagtcgc
    41581 atacctagac tagaacattt ttttttagct tcattccatt tgagagatcc ttgcaaagaa
    41641 ctccattttt tatcatccgc atttaaaagg attgtgaaag agagaaaaaa aataaagaga
    41701 gtgatttgat tcagtttcat agtgcctttc ttaattttca tttcataaaa agtcaaccca
    41761 taaaaaaaag agaaagtgag taacggattt ttcattggaa aaattatctt tgttcattgc
    41821 tctatttatt ttttaaatag agcgtgctta tcttgtattt ataaatgaat ataaaaaatc
    41881 tctcagactt cgatttcttt tggtttactt tttggagatc gtatttaccc aattctctct
    41941 ttaaattttt gaatcattat ctctggagta tctgcaacaa agaagagttc caattgttgt
    42001 ctgtgcataa aattttcgtc tgctatgtga tgtagaaagt gaatcaaacg attgtagtaa
    42061 ccttctgtat ttaaaatacc gattggcttt gtatgaaatt ttaattgtgc ccatgttaga
    42121 atttcaaaga actcttcgag tgttcctatt ccaccgggta gcacagcgaa accatctgaa
    42181 agctcataca tcatcgcttt tcgttgatgc atatttttta aaattcgtaa atcttttaaa
    42241 cctgtatgac cttcgatcgg catgagatgt tctgtaatca ccccaatcac atctccccct
    42301 tgtgctaaaa cagaattagc aacctctccc atgatcccta tgtttgctcc cccataaact
    42361 aaaccgaagt tttcttttac taaaaattct ccaaggcatt tagcattttc ataatatgct
    42421 ctattctctc caggatgagc cccacaaaac acacaaattc tcttcatact tttcaacgat
    42481 atgtatacta tcattttaaa ggaaagttta ttttttaaat tcatagtttt atttaataat
    42541 ttggaaacct tgcaggtaga agggctctaa gaactatgga attatgaaat tattttttag
    42601 agaatttacg gggaatggaa gacccttaat tattattcac ggactttttg gttcttctaa
    42661 aaattggatt actaatgcaa aggagctatc taaactagcc catgtctata cgattgatgt
    42721 aagaaaccat ggagactctc cgcatacaaa cacccacaca attcaagatt tagtttcaga
    42781 tttaaaagaa tttatagaag cgaataacat cgataagcct atcctactcg ggcattcaat
    42841 gggaggatta aattctttac tctttgccct cacctatcct gatttaattc attcattgat
    42901 tgttgtagat atagtcccaa aatcttatcc aattaaatac gaaaaagaat ttcaagctct
    42961 cacaatggat gttagtaatt atgaatctag acaagctcta gatgaagaca tgagagaaaa
    43021 acttccagat acatttatcc gccaattttt acaaatgaat ctagaaaaaa ctgaaactgg
    43081 ttataggtgg aaattaaatg
//
LOCUS       DAOQOS010000041        41498 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_379_length_41498_cov_7.063364, whole genome shotgun sequence.
ACCESSION   DAOQOS010000041 DAOQOS010000000
VERSION     DAOQOS010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 41498)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 41498)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41498
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_379_length_41498_cov_7.063364"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(50..226)
                     /locus_tag="PLF66_10850"
     CDS             complement(50..226)
                     /locus_tag="PLF66_10850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64585.1"
                     /translation="MKNKKTKNNSESSLNTKNIYTENIFSEMNTKFLDRNANEFIQSI
                     LNGNLYNQIKPSKE"
     gene            322..960
                     /locus_tag="PLF66_10855"
     CDS             322..960
                     /locus_tag="PLF66_10855"
                     /inference="COORDINATES: protein motif:HMM:NF023395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HMZ64586.1"
                     /translation="MPTVKINRDISIRVGQRIRDLRKKRGLTQEKLAELSGIDAKHIQ
                     LMESKNPSNARIDTIEAICDAFKIRIIDFFSDDSFVDQKKDFQKVDKKSKILKARSST
                     KREIIIEDNLCYAIYDENPINRGHIVILSKREIPSYFHTLPEEKKSIWEMIEKVKVFL
                     DKEYKPDGYNIGFNVGESSGQDYPYVEIHIIPRYKNDTRNPRGGIRNVIPDK"
     gene            1077..1454
                     /locus_tag="PLF66_10860"
     CDS             1077..1454
                     /locus_tag="PLF66_10860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008590370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HMZ64587.1"
                     /translation="MRNLAFAILDGNLTADPELKTIGNGKKVSTFSLAVNHDHKEEDG
                     AANNEVSYIDIEAWDKVAENCSEFLKKGRKVTILGYIKQDRWKSADGANRQRWKVIAS
                     TVRFDGFAEKANKEYRERERKAA"
     gene            complement(1697..2470)
                     /locus_tag="PLF66_10865"
     CDS             complement(1697..2470)
                     /locus_tag="PLF66_10865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012387381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HMZ64588.1"
                     /translation="MNYLSKSKIFYLFFFILPILISLSANDLLYGKVDRVKYLMGDYP
                     REGNLKVFTNEIEKNTHTLREDTIKALKQMIEAYDRDREGKSKQHIFVVSAHRSYADQ
                     KSIWEQKFKGTRKMRESIEGKTPEQIVNLILEYSSAPGTSRHHWGTDIDINALQNDYF
                     TGKGNGAFLYEWLQKNAHKYGFCQPYNELSKRNNKGYNEEKWHWSYARVANQLQKEWE
                     EAYKKKEITFTGKFSGSDVLGDRPLEYVTSINSDCKNVK"
     gene            complement(2498..3628)
                     /locus_tag="PLF66_10870"
     CDS             complement(2498..3628)
                     /locus_tag="PLF66_10870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020772889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HMZ64589.1"
                     /translation="MKRTLAFTEEHNSFRESTRKFFETEVKPFHEEWEKVGIVPREIW
                     EKAGNAGLLCPDVPEAYGGSGADFLYNVIIIEESSRAGNTGFFISLHNDIIAPYITEY
                     GNEEQKKRWLPGCVSGEKILAIAMTEPGAGSDLKSIRTTAIEKDDHFLLNGSKTFISN
                     GQLADYVIVAAKTSDDAMSLLMVERGMPGFERGRRLEKIGLKAQDTSELHFTDVKIPK
                     QNLIGKKGQGFRYLMTKLAKERLVLAVGATEATAMVHEITLRYLKEREAFGKKIGSFQ
                     HIRFKMAEMKTELEICRTFCDQAVNAIISGELTTSEASMAKWYSTEMQKRHTDECLQF
                     FGGYGYMLEYPIARAYLDARVQTIYAGTTEIMKEIIARSIGL"
     gene            complement(3625..3939)
                     /gene="trxA"
                     /locus_tag="PLF66_10875"
     CDS             complement(3625..3939)
                     /gene="trxA"
                     /locus_tag="PLF66_10875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010575035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HMZ64590.1"
                     /translation="MALAEITDSNYQQETSSGLVLVDCWAEWCGPCRMVAPVLEELSK
                     EYGGKVSIKKLNVDDNQRTAQDLGIQSIPTLLLYKNGALVDKAIGALPKNQIKNFIER
                     HA"
     gene            complement(4054..4872)
                     /locus_tag="PLF66_10880"
     CDS             complement(4054..4872)
                     /locus_tag="PLF66_10880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017857096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64591.1"
                     /translation="MALTKEQTASYIENLKEFKSYIEELKKDVSLYKVQMKKNSNKAM
                     DPYYQLALVLNSIKLINTCISINEVSLITRNLKAEDYLNTARKEVYSVLSAMEKVVGS
                     DHENGLDENRELLDAITEITPLQRLHFLKSFRKTINTLVDAYGQGSKWRWSWPEIYFK
                     LAVLTKNFFDFRAFERENDLSNPYFYVRREHYNMIIELANYTAQEYRTKFDLSTNDTG
                     DLKKSVAMLEMNRKIFQITGNTEDLDKTKTLIESLNNKIDTIENDKANAKKKKK"
     gene            complement(5047..5439)
                     /locus_tag="PLF66_10885"
     CDS             complement(5047..5439)
                     /locus_tag="PLF66_10885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64592.1"
                     /translation="MFSAKDIINLKDVFYHQGSWKNYTSISIEGSKVGIKDESSVKKY
                     VQMVGETISNSDSVCHVAKKETDHSFSSFQVLEKSCIAGHFPIEKPTSNIEIFSSHSL
                     NLEEILKITQDYFNCENLSVSTYSKATA"
     gene            complement(5485..5871)
                     /locus_tag="PLF66_10890"
     CDS             complement(5485..5871)
                     /locus_tag="PLF66_10890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64593.1"
                     /translation="MKSTKGKISKVLFTSIVLFSFVFSISFLSFKQVKSLPFVKASKA
                     LNDFHKIKDHLYSTEYNLSSFLCNTDEFRISSLEEERDYLHFHTYLIPYLTDFHHYYK
                     NKNFSLHFRSDFNYKLYSIPPPTSLS"
     gene            complement(5993..6856)
                     /locus_tag="PLF66_10895"
     CDS             complement(5993..6856)
                     /locus_tag="PLF66_10895"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HMZ64594.1"
                     /translation="MKNRFKILLVASFLLGIIYFYLHSLPIPVNPVRKSMEGGEFRIA
                     HRCGRSILPENTLFACKTIYEKNLADILEMDVHLTKDSHLVVIHDSTVDRTTNGKGKV
                     EELTYEEIRSLDAGYTFTKDGEYPFRGKGIQILELEEFFKALPNAKYYIEVKDKHPLA
                     AKILSDLIDIYNMQEKAYIGSVRESVNLSLKEMSQGRHIVFAGFLSTFKWYIGYLLSI
                     RGAISTPEVLVTPDLPHLMPLHAGFRKALKEQNIKIHIFTINDANRIRELKELGVDGV
                     MTDNPYFFETQ"
     gene            complement(7388..9091)
                     /locus_tag="PLF66_10900"
     CDS             complement(7388..9091)
                     /locus_tag="PLF66_10900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020778407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HMZ64595.1"
                     /translation="MKLLNPKKETFAHLDEKSRNLMLKTIEFFENKGKLKVLDDDHKK
                     VWYADFLEFVKKENAFAILMTPKGYGASDSRWDSNRIMAFNEILGFYGLCYWYTWQVT
                     MLGLGPIWLSKNEEIKNKAAKLLQEGEIFAFGLSEKEHGADLIGSDMMLIPNGDGTYR
                     ASGDKFYIGNGNKAAIVSTFGKVEGTGEYVFFGVNSQHPKYELKQNIVTSQNYVAEFE
                     LHDYPITEKDIMSIGREAWDASLATIAFCKFNLGWGSVGIATHAFYESINHASHRRLF
                     NQYVTEFAHIKQLFMEAYTRLVAMRMFSFRATDYMRTASATDRRYLLFNPMEKMKVTM
                     QGEEVVTALWNIIAAKGLNKDAYFEMAIRDIQMLPRLEGTVHVNMILIMRFMQNYFFK
                     PDNSLPEIPRVHSLTDDTFLFNQGATTKGFDKVVFHDYNIAYNSYNLPNLSIFKEQIA
                     AFKELLEKAGPDKAQRNDIDFMLTMGEMFTLVAYGQLILEQAKLTNEEEALIDQIFDF
                     MVRDFSKYATQLYSKPASTKEQQEHALKILRKPVLNEARFDAIYKNHILALADTYEMN
                     R"
     gene            complement(9595..9951)
                     /locus_tag="PLF66_10905"
     CDS             complement(9595..9951)
                     /locus_tag="PLF66_10905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64596.1"
                     /translation="MKLILIFFSLCIFSFPFFAKDKPKKTSNPSKNISGNWSEYQGEF
                     NSYAASDKCRSIGMRIPTLKEWKFAFNSGKMKGWESGLYWIETEPSDGNFLAVINGEG
                     GVVSNKEGIRHLRCIL"
     gene            complement(10094..10234)
                     /locus_tag="PLF66_10910"
                     /pseudo
     CDS             complement(10094..10234)
                     /locus_tag="PLF66_10910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611297.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(10389..11513)
                     /locus_tag="PLF66_10915"
     CDS             complement(10389..11513)
                     /locus_tag="PLF66_10915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64597.1"
                     /translation="MAGLSQENKFRDVFNEKGIGNLANRIYKNYPKLNKKKFVEECIE
                     NFESMSFMDRSNQICAILYKHLPKEFPDAIDILLKSQEAPLPEPGKTDWDSFIYIPQC
                     QYVSRYGKEYYDLSMNALYELTQRCSAESELRTFIEIHPEKTLGILRKWARDQSDHVR
                     RLVSEGTRPRLPLAGRIVRFQKNPKPVIELLELLKDDPSLYVRRSVANNLNDISKDHP
                     ELVIEVLNSWKKGASKERLWILQHATRGLIKQGHTGALQLLDVHTDAKIKLSEFSLDK
                     KKVKIGSELPFSFWIESKEDKTVSVVIDYRIHFVKANGETKPKVFKLRKIKLQCNSKI
                     KIETKFKARETSGRKLYTGEHKIEIQVNGKMLAENLFMLT"
     gene            11620..12144
                     /locus_tag="PLF66_10920"
     CDS             11620..12144
                     /locus_tag="PLF66_10920"
                     /inference="COORDINATES: protein motif:HMM:NF024845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HMZ64598.1"
                     /translation="MKCPTCEVKLEKVKTEYGMVAMCQSCFGHFILEKEILRSIQKEK
                     WNQLLNVNKNPNQFKRLTCPSCKNKLDIHILSKEQNSIRIDRCSNCQVIWLEKDKIED
                     LNLKKPQSKSSLEANTEELDSKAKMLLTNIKLEEAHKKVKKRKEINQSEEEDSLLSDV
                     ADVLIDGMSQIDYS"
     gene            complement(12163..12285)
                     /locus_tag="PLF66_10925"
     CDS             complement(12163..12285)
                     /locus_tag="PLF66_10925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64599.1"
                     /translation="MGNNWFRIPSEILSILIEDRKDVPTEIFESLKYEKENNSI"
     gene            complement(12738..13313)
                     /locus_tag="PLF66_10930"
     CDS             complement(12738..13313)
                     /locus_tag="PLF66_10930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64600.1"
                     /translation="MKNYKIVLIYLNRLLFLFLFVHCGSIAQYQVKPILENCLDPMLQ
                     FRVTNKYWHHKLPFSRIELYFIHIKDSEFNSILKESGKNKIEMIDFLDQEIRESQKNK
                     GNQKSRIELVRSLIPKKGVESDSKIRLIQNNSNFEDSIISMPMDLKEGYYYWSSTSYP
                     ICSMGVDKQQILFYYKNCSIDIIFTHAGGFM"
     gene            complement(13434..13997)
                     /locus_tag="PLF66_10935"
     CDS             complement(13434..13997)
                     /locus_tag="PLF66_10935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64601.1"
                     /translation="MKNKSKKATEKYGLVILAIGVGLFLYLYGEYYFFKFYISSFFRQ
                     GVSGEFYLLKELDNFREVFFLIANLLLVSQILTFSFLKIFISSYRKNRFSLIYIFVIF
                     VLWFFFFLFRAQDWVSFDSSFIRQHSLKEEKIYSYQDIIKYKKIQYRGGVVLKIEFKK
                     STLSLNQYFLNNDIEMVEKRIGFDKKE"
     gene            14205..14600
                     /locus_tag="PLF66_10940"
     CDS             14205..14600
                     /locus_tag="PLF66_10940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64602.1"
                     /translation="MPQGTAYLILIGVGIEISLMFSIAGLISSKSLLADPKEKIFGIN
                     NRLFFAIANAALCSVIEIFLIKETSIFAWVYPWRGSFPVFITAYNPFFVPAMYCYDWE
                     ISKQKKFIGGLFTLDAILLFVFASILHWI"
     gene            complement(14806..16095)
                     /locus_tag="PLF66_10945"
     CDS             complement(14806..16095)
                     /locus_tag="PLF66_10945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase"
                     /protein_id="HMZ64603.1"
                     /translation="MKSLSQIFFLILIQMTANLFAWSNHSLCTYVVTKEMPELQKADL
                     VEVETLEAFLAKEKEGLAEILNQEEEYMKKNIPFYPTLPDEINFAKSKEIDIVKSFLY
                     AIRVNPKSKLGYYLQVIPGNTPRLPLLEPRKVTLLTKDDWIMRYTFTELKKGDKVSPI
                     EVISTAADEPDYGLDLELYSDNESEFGKKYGFGEQPFGNPEIEYATQAPIHIGYYHEA
                     WIVFLFADYLKRTLPEYRMREFLALSRFAFKTGHPYWGYRFLGWGLHYIEDLTQPYHA
                     SVLPGTSVMEMIWINTLDVFGFDSLKKAAIQRISVRHEAIENYHYAILLDSLKNHTTH
                     TFMDSMQDTKKDSIYGEYNQSYVRNLIAKESKERSDRLDFLLGNTNEILAFQRDAKFD
                     LTTIPDNEATRELNSLIEEMMLSLGSHARNYIRAGLN"
     gene            16263..16520
                     /locus_tag="PLF66_10950"
     CDS             16263..16520
                     /locus_tag="PLF66_10950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004503550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4160 domain-containing protein"
                     /protein_id="HMZ64604.1"
                     /translation="MPTVFYHGKYRFYFFSNEFQATGLLEPIHIHIESGENEAKFWIE
                     PQVSLASNDGFRSKELSEIEKVIEINRNEIILKWKQHFNLK"
     gene            16531..16809
                     /locus_tag="PLF66_10955"
     CDS             16531..16809
                     /locus_tag="PLF66_10955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002774407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
                     /protein_id="HMZ64605.1"
                     /translation="MNSILLIEPRAKEVKIIEDHAIWTLLEDGREIIVPIVWYPRLSR
                     ATKKELNNFRIIGRGSGIHWEDLDEDLSIKGFILGFKGIDSQLAKQEL"
     gene            complement(16923..17084)
                     /locus_tag="PLF66_10960"
     CDS             complement(16923..17084)
                     /locus_tag="PLF66_10960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64606.1"
                     /translation="MKQAMTLNQIREKALKALAKELSPTEIARFFQMFEQGEGDYAKE
                     RKKNTSVRL"
     gene            complement(17086..17247)
                     /locus_tag="PLF66_10965"
     CDS             complement(17086..17247)
                     /locus_tag="PLF66_10965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64607.1"
                     /translation="MTLDDLVHLASAINGQANVFLSTDYDLLKKGRKYESILNLKFFN
                     PSDFIYMEK"
     gene            17667..19598
                     /gene="ftsH"
                     /locus_tag="PLF66_10970"
     CDS             17667..19598
                     /gene="ftsH"
                     /locus_tag="PLF66_10970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004779853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HMZ64608.1"
                     /translation="MNKNLKQVGFILLIILLILYGLYKGERAENKVEEISYSDFLNMI
                     EPADGIKPIGRIIITTEGKSKKQITIDREYIEGWFEDAKDKRIKPFKTVVAPLESEIL
                     TKLRKSNLNFVAKSTEENRFLNAITAIIPWLLIIGVVWFIMMKQIQSTGNKAFSFGKS
                     KAKMNMDPKVKVTFADVAGCEEAKTELSEMIDFLKDPKKFQAIGARIPTGVLLVGPPG
                     TGKTLLARAVAGESGVPFFSISGSDFVEMFVGVGASRVRDLFEQGKKNAPCIIFIDEI
                     DAVGRLRGAGLGGGHDEREQTLNQMLVEMDGFEANEGVIVMAATNRADVLDPALLRPG
                     RFDRQVIVDLPDVKGREAILTVHTKKVPLTSDISLNSIARGTPGFTGADLANLINEAA
                     LLAARKNKKRVTQEELEDARDKVMMGPERKSFFITEKEKEVIAYHEAGHAILATLLPY
                     TEPVHKVTIIPRGRALGLTQSLPAEEKHIHPKNYWLDQIVMAMGGFIAEELKFGNTST
                     GSSNDIQHATNIARKMVCEWGMSEKLGTVNYNGGNDQVFVGRDMGHTASKYYSEQFAA
                     LIDEEVRAIVQTSLNKGRDLVKKNFKKLEQIAKALLAQEIISHEELMAIVSPTSSKGN
                     SEKSSKSAKSNGELNPALT"
     gene            19600..21060
                     /locus_tag="PLF66_10975"
     CDS             19600..21060
                     /locus_tag="PLF66_10975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64609.1"
                     /translation="MAKGNPQRFSSDIVTTTQLHISSIENIRAVLFSPSLSTEAKEII
                     EGQKDDIQIQLTEIQSAYTRFIQKLVGQPMKDEVEKQISELFPDEINIQPQETEFIKT
                     KIIYEHLASFLKASETAWNNIMGMSKVFSTYPSTKPLYEKIQRENFLNLNLLNEDTRA
                     LLNLIEKNFECYIVGPSKSDKRLLVSNLIYDEGKDYKFENLFKGMPKLETIVPTQAAA
                     PTKLSDRLNILKSKTTSTQESDYYFTSIRGTKEWNRNDAYILFVKKNELKEEEKKFYS
                     SSFVILNPSDNNNINLAAALVRKNMGHSAAPKYNKMLQTLVDFAVTNLFTKDFVDLCD
                     DTHTFLYHIGPIVIYNIIQEDLVRRDFGYCHYVENNKIIKFLPEAIIKKHIIDYWAEK
                     FFDLKSAQVDAYINYSKGVSNIRESYRTFFENAASTRKRITNDPMTTEDYMRENVGKF
                     FGYRRAQVYRRFVPDNVFGLMSEINSTELVTKFKFR"
     gene            21061..21612
                     /locus_tag="PLF66_10980"
     CDS             21061..21612
                     /locus_tag="PLF66_10980"
                     /inference="COORDINATES: protein motif:HMM:NF016214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HMZ64610.1"
                     /translation="MPTIFSHGIAALSFSSIFNTQIDKIRFYFLVFFFSILPDIDVIS
                     FLLGIPYSHPLGHRGFTHSILFSILIPIPFLYFFYRNRDFKLYQICILWFAFFLSSFS
                     HAVLDAMTNGGLGVCLFYPVSYDRFFFPFRPIQVSPIGKQFFSKSGINVLKSEIIWIW
                     APSFLFFILTETINKIISISKRK"
     gene            22335..>22922
                     /locus_tag="PLF66_10985"
     CDS             22335..>22922
                     /locus_tag="PLF66_10985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64611.1"
                     /translation="MFFFTRFQMNSAINLYKSKLVFIAILFLLLSQCKLNDSPKFKGY
                     LPSIVSTRYTIGGTVTQLGSGKTIVLQNNSGNDLTISTNGAYTFSASIANNASYEVTI
                     LTQPTNQTCVLSNASGSVTSANIVNVDVTCSIGVLSSGTIINPLNLTGGVTTLNGSPC
                     GASASGCSSVSGYADSTSPDSVKYNGAEGITTDGTN"
     assembly_gap    22923..23022
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <23023..23735
                     /locus_tag="PLF66_10990"
     CDS             <23023..23735
                     /locus_tag="PLF66_10990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64612.1"
                     /translation="GTNAKVDGTGTSASLSLPRFLTTDGTYLYVSDTTNNCIRKVNIT
                     SAVVTTIVCNNTLLSDPRGLIVYNNMLYIADNSANAIRQIDLSTNALSTVISSGLSSP
                     RNMTLIGSDIYIADSGLDRVAKTTIGTWTLSTFAGSSSGYLDGTGTGAQFKNLEGITT
                     DGTDLYVADTGNHNVRKVTVPGAVVTTLAGSLSASSGYTNSSTFSTARFNNPRGITSD
                     GTNLYVFDSNNNAIRKIQ"
     gene            23735..24604
                     /locus_tag="PLF66_10995"
     CDS             23735..24604
                     /locus_tag="PLF66_10995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64613.1"
                     /translation="MNFKFYFLFSGIYLLTAFHLIAQDNLGKVKYQVESSFDITKDSA
                     QKILQNLDFEKKERTDFVFDAFDGENFLLFPGKRKIRFRMKEDEEKKVLQINTNINFI
                     NRKCPKGQSILIREKENGEINLSANQYEYLKTGNQLLNLPDLNPSQTLKSLKELDQYL
                     KKIQLPLKSELEKTFPKKWVYLPINLSKKKKYKHKVNFGYGDITVSISGGEDYIGEKF
                     LQEKWELEFQVNPKDWIDSKCKGKYCGFEKSICLFMESQNLSAKDFNPPKISPYDVLK
                     KEIPNLSKLLPSP"
     gene            25226..26353
                     /locus_tag="PLF66_11000"
     CDS             25226..26353
                     /locus_tag="PLF66_11000"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012390154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008746 - NAD(P)+ transhydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HMZ64614.1"
                     /translation="MKFGILKESGEEKRVALLPESVEALIKMGQSVIVEKGAGETAFA
                     SDEEYKKAGASIVSKKELLSQAEAVIKIQLPEAEEIEGLKSGTLLFAVLQPLYNPQLI
                     KTLNDKKISIFSMDTIPRITRAQSMDILSSQATVAGYKAVLVAASHLPHFFPMLTTAA
                     GAIPPSKILVMGAGVAGLQAIATGKRLGGVVEVFDTRPEVKEQVMSLGGKFVEVEGAA
                     DASKAGGYAVEQSEEYKNRQREAISKSAIKSDVIITTAQIPGKKAPLLITEEMVKGMK
                     AGSVIVDLAAATGGNCQLTENNKTIVAHGVTIIGNSNLPSTIPFDASKMFGKNIINFL
                     KNMQTKEGKIEINFNDEIISGTCIAHAGEVRHKPTLDLINK"
     gene            26493..26777
                     /locus_tag="PLF66_11005"
     CDS             26493..26777
                     /locus_tag="PLF66_11005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012387214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HMZ64615.1"
                     /translation="MIYMLVLSIFVGVEIISKVPAVLHTPLMSGANAIHGVVIVGAII
                     VMGEAKEDDTLSIILGFIAVILGTLNVVGGFVVTDRMLEMFKKKTPAKKD"
     gene            26781..28181
                     /locus_tag="PLF66_11010"
     CDS             26781..28181
                     /locus_tag="PLF66_11010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002774448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HMZ64616.1"
                     /translation="MLGYIEFAYFLGSLSFVIGLKMLSHPDSARKGNILAAIGMGIAV
                     ILTILGEKIHNYGWIIGALVIGSALGAISARKVKMTAMPQMVSLFNGMGGACAAVISL
                     VEFKHNPVLQTGLMNSTTAIILAGMLIGSVSFAGSMIAFGKLQGYKAIEKPVTFPLQK
                     ILNTIILIFTIGLGVFISINMQNPHDALELFYVMFGLSLLYGILFVLPIGGADMPVVI
                     SLLNSFTGVAAACAGFLYSNMVMLIGGILVGSAGTILTVVMCNAMNRSLMNVIFGSFG
                     GGGGASGSSASGDQTVREISVTDAAILMSYAKKVIIVPGYGLAVAQAQHTCHELDSLL
                     EEKGVDVRYAIHPVAGRMPGHMNVLLAEADVPYPKLFDMEEINGEFSSTDVVLVIGAN
                     DVVNPAARKDTSSPIYGMPILDVDNAANIIVLKRGMNAGYAGIENELFFDPKTKMLFG
                     DAKSSLLKLVSEVKQN"
     gene            28202..28888
                     /locus_tag="PLF66_11015"
     CDS             28202..28888
                     /locus_tag="PLF66_11015"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HMZ64617.1"
                     /translation="MERRKRISRPLPVIIFGIIFLLLPFINYINFAYHFQIPFTNVIE
                     VLRTVDIIAVILALIPFAVGIGILLVKRWGWFLFLIYSLVVLVYDALVSISDPTEPNI
                     MTLVQSAIGFAAIFYFLKPDISAPYMKMYGRGWRFQVRKPIEMDIAINDLKLRTRDIS
                     PTGFYVEWPNCPYELNQEVNISMNVDRQGLNLKAGVVRLDTAGAGFAFRSIDRESYRV
                     LEDWISRQEA"
     gene            28975..29136
                     /gene="rpmH"
                     /locus_tag="PLF66_11020"
     CDS             28975..29136
                     /gene="rpmH"
                     /locus_tag="PLF66_11020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003004764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HMZ64618.1"
                     /translation="MKRTFQPSRVKRARTHGFRSRMSTKAGRAVLARRRAKGRYKLTV
                     SDEKVGKKF"
     gene            29147..29479
                     /gene="rnpA"
                     /locus_tag="PLF66_11025"
     CDS             29147..29479
                     /gene="rnpA"
                     /locus_tag="PLF66_11025"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HMZ64619.1"
                     /translation="MTRISETLKAKEEIKSLFQKGKRIKANHISLVYASNQISLNRYL
                     FCAERFTKKAHKRNRIKRVLRALVHCIDFIFPVNVDIAIIADSRFTELDFEVRKQTLK
                     NLFLKVKV"
     gene            29476..29706
                     /gene="yidD"
                     /locus_tag="PLF66_11030"
     CDS             29476..29706
                     /gene="yidD"
                     /locus_tag="PLF66_11030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010577568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HMZ64620.1"
                     /translation="MNQIAILLIHIYKKFISPLLPGACRFYPSCSEYALQAFKEYGFL
                     KAFYLTVNRVSRCNPMSEGYFDPLPPNPNKKN"
     gene            29715..31619
                     /gene="yidC"
                     /locus_tag="PLF66_11035"
     CDS             29715..31619
                     /gene="yidC"
                     /locus_tag="PLF66_11035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004420035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HMZ64621.1"
                     /translation="MEERQGRLLLAIVLSLGVWMGVNYFLFPPQPPKKPPVANNAKDA
                     KTAEPVKDAKEVKTPDKNTTVLKPVEIKEIDPKDIKKFYVVTSSFLAEFSSLGGRIER
                     FYVKNYPDLEGNEVKIIKNESEFVSFNGGNYKGIEITRERGFDFNLTNAKEELSAPLF
                     NHINFTANYDEAKQILSFEALSPDKNYSLKKEFSFFKSENYFKYNMTITNLSKDKLVL
                     ADQTKPIYFKSFGSLGPVKGPIISEKDQVHYYRFFYIDGSFNDNLDGVSIEGFWSKAK
                     AFFGFGSVNNDPNFDTVLKKSEPVDFFGTGSRYFIAVLNPLSGNKPSGVLLDNRKGNV
                     NGVTALYENISIEPNTSVNFDYAAYVGIRELDGMSFRDKTIDPKETKSSPFSGLSEKL
                     DKSFNQGITTPFRHGIVWILKKLHNLIPNYGWCIIIFAILLKLVFYPLNQKQAESMKK
                     MQELNPQIKAINEKYDKDPAMKQQKIMELYKKNGTNPMGGCLPMLVQIPIFIALYTAF
                     SDTIDLWKSPFLWVTDLSEPDTIWTSPAILGTAGIALNVLPLIMVGTQVVQTQMTSVA
                     TDPNQKTMMYLMPLIMLYFFWTMPSGVTLYWTIQNVLSILQQFITNRFGKNTKPQTVT
                     TTTTVSKKKK"
     gene            31642..32349
                     /gene="jag"
                     /locus_tag="PLF66_11040"
     CDS             31642..32349
                     /gene="jag"
                     /locus_tag="PLF66_11040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008590199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_process: GO:0051510 - regulation of unidimensional cell
                     growth [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HMZ64622.1"
                     /translation="MLGYIFEAEGKTKSEAEEVTLSTLRLAEGDLKFETVDPGKSGFL
                     GFTAKKPAIVRAYISSKDLPAEKIIHGVVITILKKMGIEAEVVGMGDVDGKIYIELAS
                     PESGILIGKRGATLDALQFLLNLMVDPKIRHGRKIILDIESYRDKREMSLVRLSRSIA
                     SGVAKTGKSRLLEPMNPYERRIIHMALQKDEKVFTRSEGNGTYKRVRVISAKDRHKYK
                     DLETKNPELPDDDELDV"
     gene            32349..33725
                     /gene="mnmE"
                     /locus_tag="PLF66_11045"
     CDS             32349..33725
                     /gene="mnmE"
                     /locus_tag="PLF66_11045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010572994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HMZ64623.1"
                     /translation="MIDTIAAISSGNIPSAIGIIRLSGENVLSICSKVLSGKDGLLTS
                     DFIQQNPRKAIYCDFVEDDIHIDQIVFIFFKNPNSYTGEDLAEFYFHGNPILLKNALQ
                     ILFKKGARPAKEGEFTKRAFLNGKLDLTGAEAIGRLINARSKFELELAQKNVFGEIHR
                     LSSRVRSDLISLKAECEAEIDFSTEDLTYESFEQRKERIHSLYKLCTKVLDSSKRAES
                     FIEHNKIVIYGEPNTGKSSLLNSLIGKERAIVSSIPGTTRDYLAENFSLNGIPLQLVD
                     TAGIRETEDSIEKMGIQKSHTEFESANLRLFILDISKPILFDELIERYEHKIRNSILV
                     ANKLDIAHESWVKNKELFRSANIEACEVSCKTKQGIDELLNSIHSRLIQIDKSDDYVL
                     LEERNEFYFRKIQEHLKNAIDLMESAPAEIYIKEIDYALENVGYINGKIDTEEILGRI
                     FSKFCVGK"
     gene            33736..35019
                     /locus_tag="PLF66_11050"
     CDS             33736..35019
                     /locus_tag="PLF66_11050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016752580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HMZ64624.1"
                     /translation="MEEEDYREKALEYHRAHPRGKTGIVSTKATENAYDLALAYSPGV
                     AYPCLEIHSNPDLAYEYTNRGNLLGIISNGTSILGLGDIGAIAGKPVMEGKSVLFKNF
                     AGIDVFDIELNTKDADEFIRAVKLMEPTFGGINLEDIKAPECFYIEEKLIEIMDIPIF
                     HDDQHGTAIITAAAIINYLILSNKKAENVIVVVNGAGAAAIAIAKLIIHLGVESENIF
                     LVDSKGLITTRRSDLNDMKKPFARNTNANTLEDIMKNADIFIGVSKKDQVTRAMVKSM
                     AKNPLILAMANPDPEISYPDAISVRDDLIMGTGRSDYPNQVNNLLGFPYIFRGALDMR
                     ARRITLEMKLAATYALAELARQEVPSYVSDAYGGEKFQFGKKYIIPKPFDPRVYYSVS
                     SAVAEAAHASGVARIPYPGHAAYRSHLEKYRVRKI"
     gene            35021..35293
                     /locus_tag="PLF66_11055"
     CDS             35021..35293
                     /locus_tag="PLF66_11055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HMZ64625.1"
                     /translation="MPIIEEETEVSTRIDNIYEHKVILHNDDVNSFEHVEDCLMRICF
                     KTKKDAEKIALEAHNKGKAVCYEGSIEACETVAEKMGDERLTVSLA"
     gene            35304..36173
                     /locus_tag="PLF66_11060"
     CDS             35304..36173
                     /locus_tag="PLF66_11060"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HMZ64626.1"
                     /translation="MNKTIAFIGVGIMGRGLVKNLKKTNTKLKIYARNQSKIEFLKDT
                     NTEIFPTIADTVKDADFIILCLTEDEVVEKAFFEAVAEPQKAIVLDFGTSSPKLTEKM
                     QYESKKRGFRFIDSPMTGSKLAADKGEILFMVGASQEDFQACNFIWEACGKKVIHCGD
                     VGNGQLMKITLNMIQAGLAQVYMEGLILGKKLSLDLGVLHEIITNSAARSGISDFKVN
                     CILNDDYSTHFSLKNMNKDLNHALRIATENNVSLPIIHSVKSIYDAGMSHNQGEEDFI
                     SLFKINKQINNIN"
     gene            complement(36166..38595)
                     /locus_tag="PLF66_11065"
     CDS             complement(36166..38595)
                     /locus_tag="PLF66_11065"
                     /inference="COORDINATES: protein motif:HMM:NF017081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HMZ64627.1"
                     /translation="MKKLNYFFKNALVAILFFVLAKIGLTFAFIQSNVTLFWLPSGFS
                     VAILFLFGYRFTLGIAVGAFVANLTTNAPISFVLFATIANTLEPIISIYLAKTKFKIR
                     PSLLRLKDIFFFLLFSVVVSPILSSIIGSIGLCLNGMLKWSNIGYAIASWWAGDAFGI
                     LTLGSLLLSWSFISTKPHSVKRFLEAILLFSFLCFIQFMIHFEYDFYISGKYLTWFVF
                     PFLIWSAIRLEAKVSYLFAFIAMMIAVLGTVNGKGPFYTGEINSSLLLLYSFISIVMV
                     NTMVLTATLYERREKENTIINLNIERDALFNNIDDLIWGVDRNYCLVTANNAFISSIQ
                     KSTGRILEIGNSLLDQSYYTKEILEYWRKLYDQGFSGEIVKTEFRTLDSEGPVGWISL
                     IIKPIYKEKKIIGLACYGKDITEERKSEIELRRAKEEAESANAAKSQFISNMSHEIRT
                     PLNAILGFSDLLKSTHLNEIQIKYVNTILQSGNLLLVELNDLLDFGQLYLGKIQLQME
                     KVDLISVLDQVTEIHKLKLKKNIKLKLKISEKTPHYIYTDSIRLRQILIHLLSNAIKF
                     TDSGDVVIEVTHDAMEPMDKEATLLFSIRDTGIGISKENQTQIINFFSQVDQSNVRQY
                     GGIGLGLPITNKILQLMDSHLEINSELGKGSEFFFKLRVKIGEPEEASKILPSLAILK
                     EKPIQILVADDDEINLMLIRTIISRILPKAKLSEVSNGKKAVSLCQNNQFNLIFLDIQ
                     MPEMDGYDATRKIRELENYKETPIIAVTAGVTDSTRDTCFKVGMNDYASKPFTKEKIE
                     DLILKWGYKIN"
     gene            complement(38647..39030)
                     /locus_tag="PLF66_11070"
     CDS             complement(38647..39030)
                     /locus_tag="PLF66_11070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64628.1"
                     /translation="MNKIKLILFMGLISINLAAADWGSNFISSLSSGDCKGIASKDKS
                     WCTTNDCKGIASGDKSWCQTNDCKGVASKDKSWCSSPLCKAWASSDKSWCNDSDCKGV
                     ASKDKSWCSSAQCKAIASGDKSWCN"
     gene            complement(39069..>41498)
                     /locus_tag="PLF66_11075"
     CDS             complement(39069..>41498)
                     /locus_tag="PLF66_11075"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HMZ64629.1"
                     /translation="DQTKGNINIYVGPYKASLSNTDQPIIFNEKTKRFEKCSLESSIQ
                     TFAISPEGWYLVLKNPAKDNSNPKTGTVNSLTELNVGHKVNIPGPVSFPLWSGQMVNV
                     VKGHHLRSNQYLVVRVYDETAAKENWNKAVIKKSESSTGEQEFDLSKLKLGKHLIIKG
                     TEVSFYIPPTGIEVVKDKNNQYIREAVTLERLEYCILLDEDGNKRYVRGPEVVFPEPT
                     ETFIQKDGSRKYRAIELSEIKGIYIKVIAPYTEGEVGSSNYKQFKEGDELFITGRDQM
                     IYFPRKEHAIVKYEDNEVHYAVAIPLGEARYVMNRLTGEVKLRRGPDMFLPDPRFEVI
                     VRRILEPRQVGLWFPGNDRAQEVNAELRKKAIQADKASSSLRAQAAPAPAFASDELLA
                     PSFEEMEEKEQIASDSFQRRNKYTPPRYITLDTKYDGAVSIGVWAGYAIMVVGKSGER
                     KVIAGPSTYLLEFDEDLQVFSLSKGNPKSDENLVRDVYLRVANNKVSDTIDVESKDFV
                     GIQIKLSYRLNFVNDSSKWFNVENYVKFLTDRFRSVIRTAVKNYRVEEFYTNGINIIK
                     KTILGEDNSGYHFSENEMRVYDVEVTNIAIKDATIAKLMMESQQALIQESTTLTREKS
                     KLEILKFMEKVKQETSESQYTTQKAVYGFEKEELEKRLELETTRAKSNAFIEDLKLSN
                     QLRKEDLMNQINEKQLDRDKSRLEQEVFFKEQNINLSSKELETQVKAMVERAKAISPD
                     FIAALQSFNDKEQLVKIAEAMAPLAILGGESVADVFARLLSGTKLEGVFLNIGNDVLG
                     KLPIKQKDDSK"
BASE COUNT        13640 a         7307 c         7066 g        13385 t
ORIGIN      
        1 cttccgatac acccacacac tacttgtagg gaaataacac ggcagagtat cactctttac
       61 ttggttttat ttgattatag agatttccat ttaagatcga ctgaataaac tcatttgcat
      121 ttctatccag aaactttgta ttcatttcgc taaatatatt ttcagtataa atatttttag
      181 tatttaaaga agattccgaa ttgttttttg tttttttatt tttcataaat ctagcattcc
      241 cacaaatttt aatatgccgt atatatacgg tataatatct atatatagac ggttttcttg
      301 cactgtcaag tatgataccg tatgcctacg gtgaaaataa atcgggatat atcaatcaga
      361 gttgggcaaa ggattcggga tcttcgaaag aaaagaggac tcacacaaga aaaactagca
      421 gagctttccg gcatagatgc aaaacatatt cagctcatgg aaagtaagaa tccatcaaat
      481 gcaagaatcg atacaattga agcgatatgt gatgcattca agataagaat cattgatttc
      541 ttttcggatg atagctttgt agatcaaaag aaggatttcc aaaaagtaga taaaaaatca
      601 aagattctaa aagcacgttc ttcgacgaaa agagaaatca taatagagga taatttatgt
      661 tatgctatat atgatgaaaa tccgatcaat agaggacata tagtaatttt atccaaaaga
      721 gaaattccca gctactttca cacattgcca gaagaaaaaa aatcgatttg ggaaatgata
      781 gaaaaagtaa aagtattttt agataaagaa tataaaccag atggatacaa tattggattt
      841 aatgtaggtg aaagttccgg acaagattat ccgtatgttg aaattcatat catcccaaga
      901 tacaaaaacg atactagaaa tcctagaggg ggaatacgaa atgttatccc tgataaataa
      961 ttttttttga gcaaaggcgt aagtttcaaa actttttccc cataacagtg cacgaatcag
     1021 caaacttttt tctctatata tacagaacaa caaacgttca aaaaggagat tgaaagatga
     1081 gaaatttagc atttgcaatt ctagatggca acttaacagc tgatccagaa ttaaaaacaa
     1141 ttgggaatgg aaagaaggta tctaccttta gccttgcggt aaaccacgat cacaaagagg
     1201 aagatggagc tgccaacaac gaagtatcgt acattgatat tgaagcttgg gacaaagttg
     1261 cggagaattg ttctgagttt ttaaagaagg ggagaaaagt taccatcttg ggttacatca
     1321 aacaggatcg atggaagtca gcggatggag ccaacagaca acgttggaaa gtaatcgctt
     1381 ctacggttcg atttgatgga tttgcagaaa aagcaaacaa agaatacagg gaacgagaga
     1441 gaaaagcagc ctaatttccg aatctcagaa ttgtgtaaga ttttctaaat ggaaaatttt
     1501 acacaatttc ttttctactc attcttcaaa aagcgctcac attacggatg gtgaactagt
     1561 tattgcgaat ttgacagaag aaattttcat ttgaactgca atcaacacta atggtttcaa
     1621 agtgtttcct atttatcggt gtcataggtg cgaatcggtg gtaatttata atttttttca
     1681 ttacaaggaa tgaacgctat ttaacgttct tgcaatctga attgatagag gttacatact
     1741 ctagaggacg gtcacccaat acatcagatc cagaaaactt tccagtaaaa gttatttctt
     1801 ttttcttata agcctcttcc cactctttct gtaattggtt ggcaacacgt gcataagacc
     1861 agtgccattt ttcttcatta tagcctttat tatttcgttt gcttaattca ttataaggct
     1921 ggcaaaatcc gtatttatga gcattttttt gaagccattc atataaaaag gcaccatttc
     1981 cttttcctgt aaaataatca ttttgcagtg catttatatc aatgtcagtt ccccagtgat
     2041 gtctagaagt tccaggtgcg cttgagtatt ctaaaattaa atttacaatt tgctctggag
     2101 tcttaccttc tattgactca cgcattttac gagttccttt aaacttctgt tcccaaatac
     2161 tcttttgatc tgcataactg cgatgagctg atacaacaaa aatatgctgc ttactttttc
     2221 cttctctatc tcgatcataa gcttctatca tttgtttcag agccttgatt gtatcttctc
     2281 tcaaggtatg ggtatttttt tcgatttcat tggtgaatac ttttagattt ccttctctcg
     2341 gataatctcc cattagatat ttaactcggt ctactttccc atagagcaaa tcatttgctg
     2401 atagagaaat tagaatgggt aaaatgaaaa aaaatagata gaatatttta gatttagata
     2461 ggtaattcat attgatttct acattataaa atttttatta taaaccaatg cttctagcaa
     2521 tgatttcttt catgatttcg gtggttccag cgtatatcgt ttgcactctt gcatctagat
     2581 aggctcttgc aattggatat tctaacatat aaccatatcc accaaagaat tgaagacatt
     2641 catccgtatg tcttttctgc atttcggtag aataccattt tgccatagaa gcttcgcttg
     2701 ttgtgagttc gccagaaata atagcgttca ctgcttgatc acagaaagtt ctgcagattt
     2761 ctaattctgt tttcatttcc gccattttaa agcggatgtg ttggaaactg ccaatttttt
     2821 ttccgaatgc ttctctctct ttcaaataac gcagagtgat ttcatgaacc atcgcggtcg
     2881 cctccgtggc accaacagcc aataccaatc tctcttttgc taatttggtc atcaaatagc
     2941 gaaagccttg tcctttttta ccgatgagat tttgttttgg aattttcaca tctgtaaaat
     3001 gaagttcaga agtatcttgt gccttcagac caatcttttc gagacgacga cctctttcaa
     3061 agccaggcat tcccctttct accataagta ggctcattgc atcatcgcta gtctttgctg
     3121 caacgattac ataatctgcc agttgaccgt tgctaataaa ggttttagat ccattgagaa
     3181 gaaagtgatc atctttttca atcgcggttg tgcggattga tttgagatca gaccctgcgc
     3241 ctggttccgt cattgcaatc gcgagtattt tttctcccga gacgcagccg ggaagccatc
     3301 tctttttttg ttcttcgttt ccgtattcgg tgatataggg agcaataata tcgttgtgca
     3361 gagaaataaa aaaaccagta ttcccagctc tagaagattc ttctataatg atcacattat
     3421 agaggaagtc tgccccagaa cctccgtagg cttcgggaac atcagggcaa agtagaccag
     3481 cattcccagc tttctcccaa atctctctgg gaacaattcc aactttctcc cactcttcat
     3541 ggaaaggttt tacttcagtt tcaaaaaatt tacgagtaga ttctctgaaa ctgttgtgct
     3601 cttccgtaaa agcaagcgtt cttttcacgc gtgtctttca ataaagtttt tgatttgatt
     3661 ttttggtaaa gcaccaattg ctttatccac caaagctcca ttcttataga gaagaagagt
     3721 tggaattgat tgaattccta aatcctgagc agttctttgg ttatcatcta cgtttaattt
     3781 tttaatagaa actttaccac catactcttt ggataattct tctaaaacag gcgcaaccat
     3841 tctacaaggt ccgcaccatt cagcccaaca gtctactaaa actagaccag aacttgtctc
     3901 ttgttggtaa tttgaatctg tgatttctgc taatgccata aaaaactcct ttttagttcc
     3961 ttatctatta gatagttcaa tatctaatta tttctttatt tatttacaaa aggtatatat
     4021 tcaatagata tttttgaata ctaaaagaaa agattatttt ttcttttttt tagcattcgc
     4081 tttgtcattc tcaattgtat ctattttatt gttgagggac tcaatgagag tcttggtttt
     4141 atccaaatcc tctgtgtttc ctgtgatttg gaagatttta cggttcattt cgagcatcgc
     4201 aaccgatttc ttcaaatctc cagtgtcatt ggttgaaagg tcaaacttgg ttctatattc
     4261 ttgggcggta tagttggcaa gttcaatgat catattgtaa tgctctcttc gaacataaaa
     4321 atagggattg cttagatcat tttctctctc gaatgctcta aaatcaaaga aattcttggt
     4381 aagaacagct aatttgaaat aaatctcagg ccagctccaa cgccatttgc ttccttgtcc
     4441 atatgcatct acaagtgtat tgatggtttt tcgaaagctt tttaaaaaat gaagtctttg
     4501 caaaggagtg atttcagtaa ttgcatctaa tagttcccga ttttcatcga gtccattctc
     4561 atggtcactt cctaccactt tttccatagc agacaataca ctgtaaactt cctttcgggc
     4621 agtatttaag tagtcttctg cttttaaatt ccgagtgatt agggatactt cattgatact
     4681 aatacatgta ttgattagtt taattgaatt tagaactaaa gcaagttgat agtaaggatc
     4741 cattgctttg ttcgaatttt tcttcatctg aactttataa aggctcacgt ccttttttag
     4801 ttcttcaata taagatttaa actcttttaa gttttcaatg taggatgcag tctgttcttt
     4861 tgtcagtgcc atataaagat actacattta atatcgtctt tttttgggaa taatatagac
     4921 ttattccaat tgaatttaca aaaagaaacc attttcaaaa aatctgagga ttagaatcta
     4981 accctcagaa aagggggaga taaattttaa tttagccaag gaaactaaat taagataagc
     5041 caatctctaa gcggttgcct tgctgtaggt agaaacgctt aggttctcac agttgaaata
     5101 gtcctgtgtg attttgagaa tttcctcaag attcaaagaa tgggaagaaa aaatctcaat
     5161 attagaagta ggtttttcta tcggaaaatg acctgcgata caggactttt ccaaaacctg
     5221 aaaagaagaa aaagaatgat cggtctcttt tttagcaaca tgacataccg aatcggaatt
     5281 gctaattgtc tctcctacca tttgaacata tttttttacg gaggactcat ccttgattcc
     5341 tactttactt ccttcgatcg aaatgcttgt ataattcttc caactacctt gatgatagaa
     5401 aacgtctttt aaatttataa tatcttttgc actaaacata atactctcct gttttaaaaa
     5461 aatttggttt aaattgagct gattttagga aagagaggtt ggaggtggta tagaatagag
     5521 tttgtaatta aaatcagatc gaaaatgaag actaaaattt ttatttttat aataatggtg
     5581 aaaatcagtt aaatagggaa ttaggtaggt atgaaaatgt aaataatctc tttcttcctc
     5641 aagactgcta attctaaact catctgtgtt gcatagaaaa ctggaaagat tgtattcggt
     5701 tgaatagaga tgatctttta tcttatgaaa gtcatttaaa gccttagatg ctttgacaaa
     5761 agggagtgat tttacttgtt tgaaagagag aaaagaaatg ctaaaaacga atgaaaaaag
     5821 aacaatggat gtaaataaaa ccttacttat ttttcctttc gtagacttca tagtattctc
     5881 tctgtataga ctgattgatt cattcaaaaa agtcaaccac taaacaagaa tcgtttggag
     5941 aggattgtaa aatatagaca acagaatcat tacaatcaca ttacaatcat ttctattgag
     6001 tctcaaaaaa gtatggatta tcagtcataa ccccatctac acctaattct tttaactctc
     6061 taattctgtt tgcatcattt attgtaaaaa tatgaatttt aatattttgt tcttttaatg
     6121 cttttctaaa tccagcatgt aaaggcataa gatgaggcaa atcaggagta accaatactt
     6181 ccggagtaga aatagcacca cgaatcgata ataaatatcc gatataccat ttgaaagttg
     6241 ataaaaatcc ggcaaagaca atatgtctac cctggctcat ttcctttaaa gataaattta
     6301 cactttctcg aacagatcca atataggctt tttcttgcat attatatata tcaattaagt
     6361 ctgataaaat ttttgcggct aatggatgtt tgtctttcac ttctatataa tactttgcat
     6421 tgggaagtgc tttaaaaaat tcttctagtt ctaaaatctg aattcctttt cctcggaaag
     6481 gatactctcc atcttttgta aatgtatatc ctgcatctaa agaacgaatc tcttcatacg
     6541 ttagttcttc tacttttccc ttcccgttgg ttgttctatc cactgtagag tcatgaatga
     6601 ctaccagatg agaatccttt gtgagatgca catccatttc tagaatatca gctagatttt
     6661 tttcataaat tgttttacag gcaaataaag tattttccgg aagaatcgat ctaccacatc
     6721 gatgtgcgat tctaaactca ccaccttcca tagatttacg aacaggattc acaggtatgg
     6781 gtaaggaatg aagataaaaa taaatgattc ccagtaaaaa ggaagcaact aataatattt
     6841 taaatctatt tttcaagtgt agattttgat aggattctaa aaagaatcaa tacgatgaga
     6901 tcattcgttt tgtgcaatgc aatagaatat agtaaatagc ggtaactagc gaagtccaat
     6961 gctttctatt taagagaaat gaaaaagaat tagccacatt atgttgcgac ccttaggaag
     7021 caacattgag tttgagcaca gagaacacga agaaaagaga aggtttatta tttattcact
     7081 tcgcgagtct tcgtgcattc ccgcccatcg cttgaggctc accatagcca agctatgtgg
     7141 ttcaaaatcc ttaagttgac agcattaagc gaagtcgaag taacaactct cagtaacaga
     7201 aaattcaaaa gattaccacc gagaacaccg ataacgaaac gatacagaaa tgattgatca
     7261 ttttgaatta tcgtgtcttt tatcggtgcg atgcggtgag cctagtcgaa ccgttcatcg
     7321 tcttcatcgg tggtgaaaaa tccttacatc gaattcacgt tgtttgcatt acctctaatt
     7381 atgaatctta acgattcatc tcataagtat cagcaagtgc taggatatga tttttgtaga
     7441 tagcatcaaa tctagcttca tttaaaactg gctttcttaa aattttaaga gcgtgttctt
     7501 gttgctcttt tgtgctagct ggttttgaat agagctgagt tgcgtattta gaaaaatcac
     7561 gaaccatgaa atcaaaaatt tgatcaatca aagcttcttc ttcatttgtg agttttgctt
     7621 gttctagaat caattgacca tacgcaacaa gagtaaacat ttctcccatt gtgagcatga
     7681 aatcaatgtc gtttctctgt gctttgtctg gacctgcttt ttcaagcaat tctttaaatg
     7741 cagcaatttg ctctttgaag atagaaagat ttggaagatt ataactgtta tacgcaatgt
     7801 tataatcatg gaatacaact ttatcgaaac cttttgtagt agcaccttgg ttgaataaga
     7861 aagtatcatc tgtgagagaa tgaactcttg ggatttcagg aaggctattg tctggtttaa
     7921 agaaataatt ttgcataaag cgcataatta aaatcatgtt tacatggaca gttccttcta
     7981 gtctaggaag catttgaata tcgcgaattg ccatttcaaa ataagcgtct ttatttaaac
     8041 cttttgcagc aatgatattc caaagagcgg ttactacttc ttctccttgc atagtcactt
     8101 tcattttttc cattggatta aataagagat aacgtctgtc ggtagcagat gctgttctca
     8161 tataatcggt tgctctaaaa gaaaacattc tcattgcaac tagtcgagtg tatgcttcca
     8221 taaagagttg tttgatatga gcaaattcag ttacatattg attgaataaa cgacggtgag
     8281 aagcgtgatt gattgactca taaaaagcat gagtagcaat cccaacagat ccccaaccta
     8341 aattgaattt acaaaatgcg atagtagcaa gagaagcgtc ccatgcttcc cgtccaatag
     8401 acatgatgtc tttttctgtg ataggataat catgtagttc gaactcggct acatagtttt
     8461 gagaagtaac aatgttttgc tttaattcat attttggatg ttgtgaattc actccaaaga
     8521 aaacgtattc ccctgtgcct tctacttttc caaaagtaga aacgatagct gctttgtttc
     8581 catttccaat ataaaactta tccccacttg ctctataagt tccgtcgcca tttggaatga
     8641 gcatcatatc acttccaatt aagtcagccc catgttcttt ttcagataac ccgaaagcaa
     8701 agatttctcc ctcttgtaag agtttagcag ctttattttt gatttcttca tttttagaaa
     8761 gccaaatagg acctagacca agcatggtca cttgccaagt ataccaataa caaagtccat
     8821 agaaaccaag gatttcattg aatgccatga tacggttgga gtcccaacga gaatcagaag
     8881 caccatagcc ttttggtgtc atgagaatcg caaaagcatt ttcttttttt acaaattcta
     8941 agaagtcagc ataccaaact tttttgtggt catcatctag aactttgagc tttcctttgt
     9001 tttcaaaaaa ttcgattgtt tttaacataa gatttctaga tttttcatct agatgtgcga
     9061 aggtttcttt ctttgggttt aaaagtttca ttgatttatt gaatcctcta tcagttttta
     9121 gctcatacaa agcagtaaga ttctaaacag tgtttgattt gaatttcaaa ttgaaattca
     9181 tttagaactt tgtataaagc aaattaagat aaggatttta gagatttcct ttttcgtcta
     9241 tacaaaaata ggagagggat taggctcatt taaggtgata gggtaaactt ttgaaaaaag
     9301 gttacctata ttaagtgaat gagtaaagat aatgaattta tattggagct aagggttaca
     9361 attgaataat ttttgaaacc aaatatctca aaagtttaga agctaaacta aaatcaataa
     9421 ccgatacaga ataggggtaa gacacaatca accatgatat cagttgttgc tcatagtctt
     9481 tatgaaagct ataaatctta tcatactttt gtgaaagacg ataaggggaa taggacaagt
     9541 cgatccatta ccgaactgga aatatgatta aggttaatat cattttaccg caaatcaaag
     9601 aatacatcta agatgacgaa ttccttcttt gtttgaaaca actccacctt ctccattgat
     9661 gactgctaag aagttaccgt ctgacggttc tgtttcaatc cagtaaagcc cagattccca
     9721 acctttcatt tttccagagt tgaaagcaaa cttccattcc ttgagagttg gaattcgcat
     9781 cccaatactt ctacatttgt cagaggcagc ataggaatta aattcacctt ggtattcact
     9841 ccaattgcca gaaatatttt tagatggatt ggaagttttt ttaggtttat cttttgcgaa
     9901 gaaagggaaa gaaaaaatac aaagagaaaa gaaaattaga ataagtttca tgaccctatt
     9961 ttggtttgtt ggaaatttct gtcatgtatt ttttttaatc ctttccattt agaaattttc
    10021 ggggcattct taagaattac gatacccttt cttcgtatta agttgaacta ctacatattt
    10081 agtggacacg attttaggca aattgcttct catattgtaa tctataattg actgggcttg
    10141 taaagcctaa agtcgaatga aatctttttc gattgtaaaa aacttcaatg tatctaaata
    10201 gatctgattt cgcttcttgc tttgtttggt atttcaacgg acacctttta tataatccta
    10261 ttgactgcct gaaacagacc agagcacagc cttgtttcat acatttgcgg agttctaatg
    10321 tttctaaact taggattcaa tgcaaatgga tattctagac tatccagaat tatccttcaa
    10381 gaaagctatt acgttagcat aaataaattc tctgcaagca ttttcccatt gacttggatt
    10441 tcaattttat gctcgcctgt atatagcttt cttcctgaag tttctctggc tttgaatttg
    10501 gtttcaattt ttatcttgga attgcattgt agtttgattt ttctgagttt aaaaactttt
    10561 ggctttgtct cgccatttgc tttgacaaaa tgaattctat aatctatcac cactgagaca
    10621 gtcttatctt ctttagattc gatccaaaag gaaaagggaa gctcacttcc tatctttact
    10681 ttctttttat ctaaagaaaa ttctgacaat tttatctttg catctgtatg cacatctagt
    10741 aattgcagcg ctcctgtatg accttgtttt atgagcccac gagtggcatg ttgcaaaatc
    10801 cagagtcttt ctttacttgc ccctttcttc cacgaattta aaacttcaat cacaagttct
    10861 ggatgatcct ttgaaatatc atttaaattg ttcgccacgg aacgcctaac gtataaagat
    10921 ggatcgtctt tgagtaattc taaaagttca atgactggtt ttggattctt ctgaaaccga
    10981 acaattcttc ccgcaagagg aagtctaggt ctagttccct ctgatacaag tcttctcaca
    11041 tgatcggatt gatcccttgc ccattttctt aaaattccaa gtgtcttttc aggatgaatc
    11101 tctataaagg ttctaagctc actttctgca ctgcatcttt gcgttagttc atagagtgca
    11161 ttcatagaaa gatcatagta ttctttccca tacctagata catattgaca ttgaggaata
    11221 taaataaacg aatcccaatc tgtctttcct ggctctggca aaggtgcttc ttgtgatttg
    11281 agtagaatgt cgattgcgtc tggaaattct ttcggtagat gtttatacaa tatagcacag
    11341 atttgattgg atcgatccat aaaactcatc gattcaaagt tttcaataca ttcctcaaca
    11401 aatttctttt tatttaactt tggataattt ttataaatac gatttgctaa atttccaatt
    11461 cctttctcat taaaaacatc tctaaattta ttttcctgac tcagtcctgc cattttattt
    11521 cctatgcttt cttttctcca aactcttatt tataaaacaa ctttgcaaat aaaataatta
    11581 ggattgaact aaattcatat ccgtaaatac tttcacttta tgaagtgtcc tacttgcgaa
    11641 gtaaagctag aaaaagttaa aaccgaatat ggaatggttg cgatgtgtca atcgtgtttt
    11701 ggtcatttta ttttggaaaa ggaaatctta aggtcgattc aaaaggaaaa atggaatcag
    11761 ctattaaatg taaataaaaa tccgaatcaa tttaagagac tcacttgccc atcctgtaaa
    11821 aataaactcg acattcacat tttatcgaaa gaacaaaact ctattcgaat cgatagatgt
    11881 tccaactgcc aagtaatctg gttagaaaaa gataagattg aagatttgaa tttaaaaaag
    11941 ccacaatcta aatcttcttt agaagcgaat acagaggaat tagattcgaa agccaaaatg
    12001 cttttaacaa atatcaaact agaagaagcc cataaaaaag taaaaaaaag aaaggaaata
    12061 aaccaatcag aagaagagga tagcttactg tcggatgtag ctgatgtatt gatagatgga
    12121 atgagtcaaa tagactattc ttaaattcct ttctttgaat gtctaaatag aattattttc
    12181 tttctcgtat tttaaacttt caaaaatttc tgttggaaca tcttttctgt cttcaattaa
    12241 aatggataag atttcactcg gaatgcgaaa ccaattattt cccattcaaa agtagaaata
    12301 ggagcaccct tttgaattaa agtccttact aattcaggat ctccattctg ggcagcgaat
    12361 agtaaattac ttcttcccat ttttttagta ttataatttg gatcggcttt gtgtctaaga
    12421 gcagttttag aattttcaaa tttatttcca attgtcccta cttccttcta acaaaaagga
    12481 tgatatctca aaagaaataa attacacttt gttcaatgca tttagacttt cacaaggaca
    12541 tatagggcaa gatcaattat ataaagattc tgaaagaatt aaaattttta aagaatacaa
    12601 tatttcctga attcgaagaa gcggctaatg ataaaataat tgcaaactgt tctagaagga
    12661 aataactcat aatccttaat tattttgaaa ttatatttct gctcggcatt gatccttata
    12721 aatcgctgct gattcttcta cataaatcca ccagcatgtg taaaaataat atcaatggaa
    12781 caatttttat aataaaaaag aatctgttgt ttatcaacgc ccattgaaca gatggggtat
    12841 gacgttgaac tccaataata atatccttcc ttcagatcca ttggcattga aatgattgaa
    12901 tcctcgaaat tagaattgtt ttgtatgagt cgaatctttg aatcactctc gactcctttt
    12961 tttggaatta aactgcggac tagttcgatt ctggattttt gattcccttt attcttttga
    13021 ctttcacgaa tttcttgatc tagaaaatca atcatttcaa ttttattttt cccagattct
    13081 ttcagaatag aattaaattc agaatctttt atatgaataa aatataactc tattcgagag
    13141 aatggtaatt tatgatgcca gtatttattg gttactctaa attgaagcat tggatctaaa
    13201 caattttcaa ggatcggttt cacttgatat tgggcaatgg aaccacaatg aacaaataag
    13261 aaaaggaata ataatctatt taggtaaatc agaacaattt tataattttt catataatat
    13321 atctcaacaa ccccttaaaa aaaaccagat gtttggtata ccagaatcat ctattgcttt
    13381 aaactagtat tcctatattt ctaatagcga tttcatttcc tttctcacaa acgttattcc
    13441 ttcttatcga aaccaatccg tttttctacc atctcaatat cattgtttag aaaatattga
    13501 ttcagagaaa gagtagattt tttaaattca atctttaata caacgcctcc tcgatattgg
    13561 atctttttat attttataat atcttggtaa gaataaattt tttcttcctt taaactatgt
    13621 tgccgaatga aacttgaatc aaaagaaacc caatcctgtg ctcgaaataa aaagaaaaag
    13681 aaccataaaa caaaaatcac aaaaatataa atcaaactaa accgattctt tctataacta
    13741 gaaataaata ttttgagaaa ggaaaaagta agaatctgac tgactaaaag tagattggct
    13801 ataagaaaaa aaacttctct gaaattatct aactctttta gtaaataaaa ttccccagag
    13861 accccttgtc tgaaaaatga agagatatag aacttaaaaa agtaatattc tccatacaga
    13921 tataaaaata atccaacccc aattgcaaga atcacaagac catacttctc agtagctttt
    13981 ttagatttat ttttcacgaa aatgattcca tttttaaact aaataaacaa ttgaaaaatc
    14041 aaattttttc ttatgaatta taaattttac acatgcaata gattgcgctt tatcatttaa
    14101 attggaaggg gattgcttcg gggctttgtc tctatgcaat ccattggttt tttgaaattc
    14161 taaatgcctt aattcaacat ttcagtggtc atgctttatg gatcgtgcca cagggaactg
    14221 cttatctcat tttgattggt gttggaattg aaatttctct catgttctcg attgcaggtc
    14281 tcattagcag caaatcacta ttagccgatc caaaagaaaa aatctttgga ataaacaatc
    14341 gccttttttt cgctattgca aatgccgccc tttgttctgt gattgaaatc tttctaataa
    14401 aagagacaag catatttgcc tgggtttatc cttggagggg atcttttcct gtctttatca
    14461 ctgcctataa tccttttttc gttccagcca tgtattgcta tgactgggaa atatcgaagc
    14521 agaaaaaatt catcggcgga ctttttacct tggatgcaat tcttttattt gtgtttgctt
    14581 ctatactgca ttggatttag tgaacttgaa ttcgatgtaa aaatatttaa ccaccgataa
    14641 cgaaaccata cagaaacgat cgataatttt gaattatcat atcttttatc ggtgtcctca
    14701 gtgtctatcg gtggtaatct tttaaattct ttgctgtatc gaactctcgt taatttatag
    14761 tttatatact cccgataaaa aatttcggga gtgaccacat tgtcttcaat ttaatccggc
    14821 gcggatgtag ttgcgtgcat gagagcctaa cgataacatc atctcctcta tgagtgaatt
    14881 taattctcgt gtagcttcgt tgtctgggat tgttgttaaa tcaaattttg catctctttg
    14941 aaaggctaaa atttcatttg tatttccgag tagaaaatcc aatcgatctg atctttcttt
    15001 ggattccttc gcaatcaaat ttcgaacata gctttgattg tattcaccat agatagaatc
    15061 tttcttagta tcttgcattg aatccataaa tgtatgagtt gtatggtttt tcaaagagtc
    15121 gagtaaaatt gcatagtgat aattttcgat tgcttcatga cgaacactaa tcctttgaat
    15181 cgccgccttc tttaaagaat caaatccaaa cacgtctaac gtattgatcc aaatcatttc
    15241 catcacactc gttccaggga gaacagaagc atgatagggc tgagtcaaat cttcaatata
    15301 atgaagtccc catcctaaaa agcgataccc ccaatacgga tgaccggttt taaaagcaaa
    15361 gcgagagaga gctaaaaatt ctctcattcg atactctggt aaagtccgtt ttaaatagtc
    15421 tgcaaataaa aaaacaatcc aagcctcgtg ataatatcca atgtgaatag gagcctgtgt
    15481 tgcatattca atttctggat taccaaaagg ctgttctcca aaaccatatt tctttccaaa
    15541 ttctgattca ttatcagagt ataattctaa atcgagtcca tagtcaggtt catctgctgc
    15601 tgtgctaatc acctcaatcg gagatacctt atctcccttc ttaagctcag taaaagtata
    15661 acgcataatc caatcatctt ttgtaagaag tgtgactttt cttggttcca gtagcggcaa
    15721 acgaggagta ttccctggaa tcacttgtaa ataatagcca agcttactct ttgggtttac
    15781 tcgaattgca taaagaaagc tttttacaat atctatttct ttcgattttg caaaattaat
    15841 ttcatctggt agtgttggat agaatggaat attttttttc atatattcct cttcttggtt
    15901 tagaatttct gccagtcctt ctttttcctt cgctaaaaaa gcttctaagg tttccacttc
    15961 caccaaatct gctttttgca gttctggcat ttcctttgtt acaacatacg tgcagagact
    16021 atgattcgac catgcaaata gatttgcagt catttgaata aggattagaa aaaagatttg
    16081 tgatagagat ttcatttttg tcctcgtaga acgggaaacg attccccgtc tacgaggaat
    16141 tattattgcc tacgcgtcat aacttgactt gtctgtgaaa ttataaaacc attttttatt
    16201 tacactcttc attgtattgg gtttcaactt gaactataaa ataaaggcat gaaattggtt
    16261 ctatgccgac agttttttat catgggaagt atcgttttta ttttttctcg aatgaattcc
    16321 aagcaacggg tcttttagaa ccgattcata ttcacattga aagtggtgaa aatgaagcca
    16381 aattttggat tgagccgcaa gtctctctcg cctctaatga cggctttcgc agtaaagaat
    16441 tatctgagat tgaaaaagtg attgaaatta atagaaatga aattattcta aaatggaaac
    16501 agcacttcaa tttgaagtaa tggaattaaa atgaattcta tcttattaat agaacctaga
    16561 gcaaaagaag ttaaaattat cgaagatcat gctatttgga ctttgttaga agatggcagg
    16621 gaaataattg ttcccatagt atggtatccg cgcctttcaa gagctacaaa aaaagaactg
    16681 aacaatttca gaattattgg acgcggttca ggaatccact gggaagattt agatgaagat
    16741 ttaagtatta aaggttttat attaggattc aaaggaatag atagccaact cgctaaacaa
    16801 gaattataga ggaaataaaa agatttttta cgcgacttta cgctttttgg aatttgtttt
    16861 tattgaagcg gattttctct tagatttttt tcgagaagat aaaacatctt ggtaaatatc
    16921 gtctatagtc ttaccgacgt attttttttt ctttcctttg cataatcccc ttcaccttgt
    16981 tcaaacattt gaaaaaagcg ggcaatttct gttggagaca attctttggc gagtgccttt
    17041 aatgcttttt ctctgatttg gtttagtgtc attgcttgtt tcattttatt tctccatata
    17101 aataaaatcg gaaggattaa aaaattttag atttaaaatc gattcatatt ttctgccttt
    17161 cttaagtaaa tcataatcag tagaaagaaa tacatttgct tggccattga ttgcagaagc
    17221 taaatgcacc aaatcatcaa gtgtcacggt cagcggttac aatgcacatg gttatccttg
    17281 cccttagtag tagtccgaca ttcaacggag caaaaatagg aatttttgca gagagtaaaa
    17341 tttgtccgat tttattctcc gtgtttgaaa tcttctgaat ctctgtatac caaacttccc
    17401 ctaaacttag gggtaaagat tgggcaaagt tcccttaaac ttatccttac agggatcttt
    17461 aggagaagtt tagtcctatc ttttggggat ttttgcatat atcgaatcgg gaacgaattt
    17521 ccctgctctt gcatatgcaa gcccataaaa gttcttacgg aacttttatg ggtctaaaag
    17581 atatgcaggt aagggaggga gggtgtattt tatcttgcaa ttatttgata aatcagaaaa
    17641 ctaacattca cgggagaatt ttaatgatta ataagaatct caaacaggtt ggatttatcc
    17701 tcctgattat actgctgatc ctttatgggc tttataaagg ggaaagggca gaaaataaag
    17761 tagaggaaat ttcatactct gactttttaa atatgatcga acctgctgat ggaattaaac
    17821 caattggacg tattattatt accacagaag gtaagagtaa aaaacaaatc acaattgatc
    17881 gagaatatat cgaaggttgg tttgaagacg ctaaagacaa aagaatcaaa ccttttaaaa
    17941 cagtcgtcgc tccactcgaa tctgaaatac taactaaact acgaaaatca aatctcaatt
    18001 ttgttgccaa gtcaaccgag gaaaaccgat tcttaaatgc aatcacagcc attattccat
    18061 ggctactgat tattggtgtt gtttggttca tcatgatgaa acaaattcaa tccacaggaa
    18121 acaaagcttt cagctttgga aaaagcaaag caaaaatgaa tatggatcca aaagtaaaag
    18181 taacattcgc tgatgttgct ggatgtgagg aagcaaaaac agaattatca gaaatgattg
    18241 actttctaaa agatcctaaa aaattccaag cgataggcgc tcgaattccc actggagtct
    18301 tactcgtagg tcctcccgga acaggtaaaa ccttgttagc ccgcgcggtt gcaggagaat
    18361 ctggtgttcc tttttttagt atctctggtt ccgatttcgt agagatgttt gttggggtcg
    18421 gtgcttctcg tgttcgtgat ttattcgaac agggaaagaa aaacgctcct tgtattatct
    18481 ttatcgatga gattgatgct gtaggtcgtc tgcgtggagc agggcttggt ggcggacatg
    18541 atgaaagaga acaaactctc aatcaaatgc ttgtggaaat ggatggcttt gaagcaaacg
    18601 aaggtgtaat cgttatggca gctaccaacc gcgcagatgt tctcgatcct gcccttctac
    18661 gtccgggtcg ttttgatcgt caagtgattg tagaccttcc tgatgtaaaa ggaagagaag
    18721 caattctaac ggttcatact aaaaaagttc cacttacgag tgatatatca ctcaactcta
    18781 tcgctcgcgg aacccctgga tttacaggag cagacttagc aaatcttatc aatgaagcgg
    18841 cacttcttgc tgctcgtaaa aataaaaaac gagttacaca agaagaatta gaagatgctc
    18901 gcgataaagt gatgatgggt cctgaaagaa aatcattctt catcacagaa aaagaaaaag
    18961 aggtcattgc ataccatgaa gctggacatg cgatactcgc tacacttctt ccttacacag
    19021 aaccagttca taaagttaca atcattccaa gaggacgtgc gttaggtctc acacaatctc
    19081 ttcctgcaga agagaaacat attcatccaa aaaattattg gctagatcaa attgtaatgg
    19141 ctatgggtgg atttatcgca gaagaattaa agtttggaaa tacttcgact ggttctagta
    19201 atgatattca acacgcaacc aatattgctc gtaaaatggt ttgcgaatgg ggaatgtctg
    19261 aaaaactagg aaccgtcaat tacaacggtg gaaacgatca agtttttgtt ggacgcgata
    19321 tgggacatac tgctagcaag tattattcag aacagtttgc ggctctcatt gatgaagaag
    19381 ttcgtgccat tgttcaaacc agtttaaaca aaggtagaga tttggtgaaa aagaatttca
    19441 agaaattaga gcaaattgca aaagctctac ttgcacaaga aatcatttct catgaagaac
    19501 tcatggcaat cgtttctcct acaagttcaa aaggaaattc agagaagtct agcaaatctg
    19561 ctaaatcaaa tggagaacta aatcctgctt taacataata tggcaaaggg taatccacaa
    19621 agattttcat cagatattgt tacaacgacg caattgcata tcagctcgat tgaaaacatt
    19681 cgagctgtgc tttttagtcc tagcctttcc acagaggcaa aagaaattat tgaaggtcaa
    19741 aaagatgata ttcaaatcca attgaccgag atccaatctg cctatactcg ctttattcaa
    19801 aagctagttg ggcaaccaat gaaagatgaa gtagaaaaac aaatttcaga actttttcca
    19861 gatgaaatta atatccagcc acaggaaact gagtttatta aaacaaagat tatctatgaa
    19921 catttagctt cctttctaaa agcttccgag actgcttgga ataatataat gggtatgtca
    19981 aaagtttttt caacctaccc ttctactaaa cctctctatg aaaaaatcca aagagaaaat
    20041 tttttaaatc taaacctttt gaatgaggat acacgagcct tactcaatct aatcgagaaa
    20101 aattttgaat gttacattgt aggtccatcc aaatcagata agcgcttact ggtaagtaat
    20161 ttaatttatg acgagggaaa agattataaa tttgaaaacc tcttcaaagg aatgcctaaa
    20221 cttgaaacga tagttcctac acaagcagca gctcctacaa agctttctga ccggttgaat
    20281 attttaaaat ctaaaactac atcaacacaa gaatccgatt attatttcac atccattcgt
    20341 ggaacaaaag aatggaatcg caacgatgca tatattttat ttgtaaagaa aaatgaactc
    20401 aaagaagaag aaaagaaatt ttatagttct agctttgtga ttctaaatcc atctgataat
    20461 aataacatca acctagctgc tgcactcgtt cgtaaaaaca tgggtcactc cgctgctccc
    20521 aaatacaata aaatgcttca gactctagta gactttgcag ttacgaattt atttacgaaa
    20581 gactttgtag atctctgcga tgacacacat acatttttat accatatagg acctatcgtt
    20641 atttataata tcatccaaga agatttggtt cgaagagatt ttggttattg ccactatgtt
    20701 gaaaataata agattattaa atttctaccg gaagcaatca tcaaaaaaca tatcatagac
    20761 tactgggctg aaaaattctt tgatttaaag tcagcacaag tggatgcata tattaattat
    20821 tccaaagggg tttccaatat tcgggagtcc tatagaactt tttttgaaaa tgctgcatct
    20881 actcgaaaga gaatcacaaa tgatccaatg accactgaag attatatgag agagaatgta
    20941 ggtaaatttt tcggatacag aagagcccaa gtctatagaa ggtttgttcc agataatgtt
    21001 ttcggattga tgtcggaaat taattctact gaacttgtta caaagtttaa attcagatag
    21061 atgccaacta tattctcgca tgggatagcc gctctatcct tctcaagtat ttttaataca
    21121 caaatagata agattcgttt ttattttcta gtttttttct tttctatttt accagacatt
    21181 gatgtcatct cttttctatt gggtataccc tactctcacc cgttagggca tagaggattt
    21241 actcattcga ttttattttc catacttatt cctattccgt ttttatattt tttctatcgc
    21301 aatcgagatt ttaaactata tcaaatatgc attctttggt ttgctttttt tctctcatct
    21361 ttctctcatg cagtattgga tgcaatgaca aatggaggtc ttggagtctg cttattttat
    21421 cctgtttctt atgatcgatt tttttttccc tttcgcccca ttcaagtttc tccaatcgga
    21481 aagcaatttt tctctaaatc tggaattaac gttttaaaat cagaaattat ttggatttgg
    21541 gctccctcat ttcttttttt tattctaact gagacgataa acaaaatcat ttctatttct
    21601 aaaagaaaat gactcatatc ttttcctaac tgatcttacg caaaatttat catttgagaa
    21661 ataaagtatg attattaaaa tattagagaa tggtaatgaa ttaataaaat caacagccct
    21721 ctctcttaag ttgactgcat tgggcgacaa gccctcctta ggacgagact tcatttaaac
    21781 atttagctca ccttgtaaaa ggggagctat tattttattt cttgtatttt gcaatttacc
    21841 acatttgatt cttagttttt ctattttaaa aaatgaatag taaatcgcta gaataccata
    21901 ctagaacatt gagcaaatcc attcctaaaa tggtttatac gcgcaaaagg gtagtttctc
    21961 ttagttggag aaatctattt caaaaatcaa taaaagagat cgttagacta acaaagagag
    22021 aatcaatcca ttcctttaag atgattttta tcaaggtgaa ttaggagttc ctacccgaat
    22081 agtccttgac aaattcaaaa gtatataggt atacattaga tagatacaag aataaattca
    22141 attaacgttt tggaagttga tttggtattg agtatggaaa tgaaaagaat cactgagatt
    22201 tatatcttat aaacaggatt gtctttattg atcttgttgg aattcaatct tcaaaacaaa
    22261 gcgtaaaatt tttcataaca ttaaaacctt ttttaaataa ctcaattacc ttttatatgc
    22321 aaaaggatgt aggaatgttt ttttttacta gatttcaaat gaattcagca atcaatcttt
    22381 ataaaagtaa gttagtcttt atagctatct tattcttact tttatctcaa tgcaagctga
    22441 atgattctcc aaagtttaag ggttatcttc cttctatcgt ttcaacacgt tatacgattg
    22501 gcggaactgt aactcaattg ggttcaggaa aaacgattgt attacaaaat aattcaggaa
    22561 atgatctgac catatcaaca aacggggcat ataccttttc agcctccatt gcaaacaacg
    22621 catcttatga agttacgatt ctaacacaac ccacgaatca aacctgtgta ttatcaaatg
    22681 catcaggatc tgttacctct gcaaatattg taaatgttga tgtaacttgc tccattggag
    22741 ttctaagtag cggaacgata atcaatccat tgaacctcac aggcggagtg acaaccctca
    22801 atggatcacc ctgtggagct agtgcttctg gatgttcaag tgtcagcggg tatgcagact
    22861 ctacaagtcc agattcagta aaatataatg gcgctgaagg aatcaccacg gatggaacaa
    22921 atnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    22981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnatggaaca aatgcaaaag
    23041 tagatggaac aggaacttct gcttcattaa gtttaccgcg ctttttaaca acagatggaa
    23101 cttatcttta tgtttcagat acaacaaata attgtattcg aaaggttaat attaccagtg
    23161 cggtagttac aactatagta tgtaataata ctttactatc tgatccacgg ggactaatag
    23221 tatacaataa catgctatat atagcagaca attcagcaaa tgctattagg caaatagatt
    23281 tatcaactaa cgctttatca acagttattt cgtctggact tagttctcct agaaatatga
    23341 ctttaatagg atcagatatt tacattgctg attcaggact cgatcgtgtt gcaaaaacta
    23401 caatcggaac atggactctt tctacatttg caggtagttc ttcaggctat ctggatggaa
    23461 cagggacagg agctcaattt aaaaatctag aagggatcac aacagatggg acagatcttt
    23521 atgttgcgga tacaggaaat cataacgttc gaaaagttac tgtccctgga gcagtggtaa
    23581 caacactggc aggttctcta tcagcctctt ctggatatac aaactcatca actttttcta
    23641 cagctcgctt taacaacccg agaggcatca cttccgatgg aacgaatctt tatgtatttg
    23701 attcaaataa caatgcaatc cgaaagattc aatagtgaac tttaaatttt actttctctt
    23761 tagcggtata tatctattaa ccgcttttca tttaatcgca caagataatt tggggaaagt
    23821 aaaataccaa gtagaatcca gtttcgatat tacaaaagac agtgcccaga aaattttgca
    23881 aaatctagac tttgagaaaa aagagagaac tgattttgta tttgatgcat ttgatggaga
    23941 aaactttctt ctatttccag gcaaaagaaa aatccgtttt agaatgaaag aggatgaaga
    24001 aaaaaaagtt ctgcaaatca atacaaacat taactttatc aatcgaaaat gcccgaaagg
    24061 gcaatccatt ctaattcggg aaaaagaaaa tggggaaatc aacctttctg caaatcagta
    24121 tgaatattta aaaacaggaa accaactttt aaatctacct gatttaaatc ccagtcaaac
    24181 cttaaagtct ctaaaagaac tagatcaata tttaaaaaaa atccaattgc ctctcaaatc
    24241 cgaattagaa aaaacatttc caaaaaaatg ggtctatcta ccaatcaatc tctcaaaaaa
    24301 gaaaaaatat aaacacaaag tcaattttgg gtatggagat attaccgttt ctatctccgg
    24361 tggagaagat tatataggag aaaaattttt acaggaaaaa tgggaattag aatttcaagt
    24421 aaatcccaaa gattggatag attctaaatg caaaggtaaa tactgcggat ttgagaagag
    24481 tatttgtctt tttatggaaa gccaaaacct atccgcaaaa gacttcaacc ctcccaagat
    24541 ttccccctat gatgttttaa aaaaagagat accgaattta tctaaattac tcccaagtcc
    24601 ctagccaaaa ctcatttcta ttcctcgaaa attcctccat ctaccctatt ttgcaaaatt
    24661 catttcccta aatccttttg attccattct cttctgagtt aaatgaactt gaattcggta
    24721 taaggaattg ataaattgtt cagaccgtca ttcccgaaat tccaccagca atcgcagcaa
    24781 ccgccaaacg atatcgggaa tctgttttct agatacccct gataatcgcc cctgcggaac
    24841 tggatgcgaa tagcatcggg tggaatttgt gggtgacaaa gtgaaacctc atcttatatc
    24901 gaactcacgt taaattacaa agactaccct actcaaaccc gttaacccaa atccccatct
    24961 cccaagagag cctagaaaaa gaggaagcca ggttagtgta aagaccagca aaggaatgtc
    25021 ggtctaccat aaacacccaa aaaaaaggga tctaaagtag aacgattgcc tatcttttaa
    25081 aaattttaac tcttccagcc tccttccatc cgaatatcct tggttacttt tctatgggca
    25141 ctctggtttt gtccaaatct aagttctgtt ttaggcttgc ttctataggt tgtacaaaaa
    25201 accttgcggt agagattaaa aaaatatgaa atttggtatt ttaaaagaaa gtggggaaga
    25261 aaaaagagta gctcttcttc ctgagtctgt ggaagccctt atcaaaatgg ggcaatcggt
    25321 gattgtggaa aagggagctg gtgaaaccgc ttttgcctct gacgaggaat acaaaaaagc
    25381 aggggctagt attgtgtcca aaaaggaact tctatcccaa gcggaggcag ttattaaaat
    25441 acagcttccc gaagcggaag aaatcgaagg actaaagtct ggaactcttc tgtttgcagt
    25501 cttgcaacct ctctacaacc ctcaattgat taagacttta aatgataaga agatatctat
    25561 atttagcatg gacacgattc ctagaattac tcgtgcccaa tctatggaca ttttaagttc
    25621 ccaagcaacc gttgctggct ataaggcagt tctagtagct gcaagccatt tgccacattt
    25681 ttttccaatg cttacaaccg cggctggagc aatcccaccc tctaaaattt tagttatggg
    25741 tgctggtgtt gctggtctcc aagcgattgc aactggtaag agattgggcg gagttgtaga
    25801 agtattcgac actagacccg aagtaaaaga acaagtcatg tctctcggcg gaaaattcgt
    25861 agaagtagaa ggtgcagccg atgcatctaa agctggagga tatgcggttg aacaatcaga
    25921 agaatacaaa aatcgccaaa gagaagccat ttctaagtct gccatcaaat ccgatgtaat
    25981 catcactaca gcccaaatac caggtaaaaa ggcacctcta ctcataaccg aagaaatggt
    26041 gaagggaatg aaagcaggtt ccgtcatcgt agatttggct gccgcaacag gtggaaattg
    26101 ccaattgaca gaaaacaata aaactattgt tgcacatggt gtcaccatca ttggaaattc
    26161 aaatcttcct tctacaattc cgtttgatgc gagtaagatg tttgggaaaa atataatcaa
    26221 cttcctaaag aatatgcaaa ccaaagaagg aaagattgaa atcaatttca atgatgaaat
    26281 catctctgga acttgtatag cacatgcagg agaagttcgt cataaaccaa cactggactt
    26341 aatcaataaa tgattggtgt ttcacttata gttaataacc catattggct agactctgag
    26401 tataaaaact ctctagttct attcctttgt ggtaattctt tttaggagat attatggatc
    26461 aaatactaaa ctttttaatc gagaacaaag caatgattta tatgcttgtt ctatcaatat
    26521 ttgtgggagt agaaattatt tctaaagtcc ctgcagtttt acacactcct ctcatgtctg
    26581 gagcaaatgc gattcacgga gttgtgatcg tgggtgcaat tatcgttatg ggagaggcga
    26641 aagaagatga tacactttct atcattttgg ggtttatcgc tgttatcctc ggaaccttaa
    26701 acgtagttgg tggattcgtt gttacagata gaatgctgga aatgtttaag aagaaaactc
    26761 cagctaagaa ggattaaccc atgttaggat atattgaatt tgcttatttt ttaggttccc
    26821 tttctttcgt aattggtctt aagatgctta gtcatccaga ctcagctaga aaaggaaaca
    26881 ttctcgcggc tatcggtatg ggaatagctg tgattctcac aattctcgga gaaaaaattc
    26941 acaattacgg gtggatcatt ggtgctctag tcattggttc agcgttaggt gcaatttccg
    27001 ctcgaaaagt caaaatgact gcaatgccgc agatggtcag tcttttcaat ggtatgggtg
    27061 gtgcttgtgc tgccgttatt tcccttgtag agtttaaaca caatcctgtt ttacaaactg
    27121 gacttatgaa tagcacaaca gcaatcattc ttgctggtat gctgattgga tctgtttctt
    27181 ttgctggatc tatgattgca tttggaaaat tacaaggcta taaagcaatt gaaaagccag
    27241 taacttttcc attacaaaaa atattaaata caatcattct aatttttaca attggtcttg
    27301 gtgtgtttat ctcaatcaat atgcaaaatc cacatgatgc actcgaatta ttctatgtaa
    27361 tgtttggtct ttccttatta tatggaattt tatttgtatt accaattggt ggtgcagaca
    27421 tgcctgttgt aatttctcta ctcaactctt tcacaggagt tgcggctgct tgtgctggat
    27481 tcttatatag caatatggta atgctgattg gtgggatttt agttggatct gcaggaacca
    27541 ttctaacagt tgttatgtgt aatgcaatga accgctctct catgaacgtt attttcggta
    27601 gcttcggcgg tggaggcggt gctagtggta gttctgcttc tggagaccaa actgttcgag
    27661 aaatttctgt aaccgatgct gctatcctaa tgagctacgc taagaaagtg atcatcgttc
    27721 ctggatacgg acttgcagtt gcacaagcac agcatacctg tcatgaacta gatagcttac
    27781 tcgaagagaa gggtgtagat gtgcgttacg cgatccatcc tgtggctggt cgtatgcctg
    27841 gtcatatgaa cgttttactc gcagaagcag atgttcccta cccaaaatta tttgatatgg
    27901 aagaaatcaa tggagaattc tcctctactg atgttgttct agttattgga gcgaatgatg
    27961 ttgtaaaccc tgcagccaga aaagatacat ctagtccaat ttatggaatg ccaatcttag
    28021 atgttgacaa cgctgccaat attatcgttc tcaaaagagg gatgaatgct ggttatgctg
    28081 gtatcgaaaa cgaattattc tttgatccaa aaacaaagat gctttttgga gatgcaaaat
    28141 cttctctttt aaaactcgtc tccgaggtaa aacaaaatta acccctaggt ggaatttgga
    28201 tatggaaaga agaaaaagaa tttcaagacc tttacctgta attatattcg gaatcatctt
    28261 tttactgctt ccgtttatta actacattaa ctttgcatac catttccaaa ttccatttac
    28321 aaatgtaata gaggttttga gaaccgtaga catcattgct gtcattcttg cgttgattcc
    28381 ctttgcagtt ggaattggaa tcctgcttgt taagcgatgg ggttggttct tatttttaat
    28441 ctactcttta gtagttttag tttacgatgc actggtctct atttcagatc caacagagcc
    28501 aaacatcatg actcttgtgc aaagtgcgat cggatttgct gccatttttt attttttaaa
    28561 accagacatt tcggctccct acatgaaaat gtatgggaga ggttggagat tccaagttag
    28621 gaaaccaatt gaaatggaca tagcaatcaa tgacttaaag cttagaacta gagatatttc
    28681 tcctacagga ttctatgtag aatggcccaa ttgcccttat gaactcaacc aagaagtaaa
    28741 tatatcaatg aatgtagata gacagggatt gaacttaaag gcaggagtag ttcgattgga
    28801 tacagccgga gctggctttg cattccgatc tattgacaga gaaagttata gagttttaga
    28861 ggattggatt tcgagacaag aagcatgaat ttcattgact cataggcaag cgaaaataac
    28921 ctgactttgt acacgaattt attatactgt aatattattt tttaaggtaa taagatgaaa
    28981 agaacatttc aaccaagtag agttaaaaga gcaagaacac atggctttag atcccgtatg
    29041 tctacaaaag cgggaagagc tgtactcgca agaagaagag ctaaaggcag atataagcta
    29101 actgtttccg atgaaaaagt cggaaagaaa ttttaattag aggttcttga ctaggatatc
    29161 ggaaactcta aaggcaaaag aagagattaa aagtcttttt caaaaaggta agagaatcaa
    29221 agccaaccat atatctttag tgtacgcatc caaccagata tcgcttaata gatacctctt
    29281 ttgtgcagaa cgttttacca aaaaggcgca taaaagaaat agaattaagc gagtcttgcg
    29341 cgcattggtt cactgtatag actttatctt ccctgtgaat gtagatatcg caatcatagc
    29401 agattctagg tttacagaac ttgattttga agtcagaaag cagactttaa agaatctatt
    29461 tttaaaagta aaagtatgaa tcaaatagcg atacttttga ttcatattta taaaaaattc
    29521 atatctcctt tattgccagg tgcttgcaga ttttatccga gttgttcgga atatgcatta
    29581 caggcattta aggagtatgg ttttttgaaa gccttctacc taaccgtaaa tagagtttct
    29641 agatgtaacc caatgtcaga agggtatttt gatcccttac ctccaaatcc aaataaaaaa
    29701 aattagagga aatcatggaa gaaagacaag gtagattatt acttgcaatc gtacttagct
    29761 taggagtctg gatgggagtt aactactttt tattccctcc gcaacccccc aaaaaaccgc
    29821 ctgttgcaaa taacgcaaaa gatgcaaaaa cagcagaacc agtaaaagat gctaaagaag
    29881 ttaaaactcc agataaaaac acaactgtct taaagccggt cgaaatcaaa gaaattgatc
    29941 caaaagatat aaagaaattc tatgttgtaa catcttcttt tctagctgag tttagttctc
    30001 tgggcggtag aatcgaaaga ttttatgtaa agaactatcc ggacttagaa ggaaatgaag
    30061 ttaaaatcat aaaaaatgaa agcgaatttg tttccttcaa tggtggaaat tacaaaggga
    30121 ttgagatcac tcgagagaga ggatttgatt ttaacttaac aaacgcaaaa gaagaattat
    30181 ccgctcccct attcaatcat attaatttca cagcaaacta tgatgaagca aaacagattc
    30241 tcagtttcga agcgttatct ccagataaaa actactctct aaagaaagaa ttctcctttt
    30301 ttaaatcaga aaattatttt aaatacaata tgactatcac taatctttct aaagacaaat
    30361 tggtgttagc tgatcaaaca aagcctattt attttaaatc tttcggaagc ttaggtccag
    30421 taaaaggtcc tattatttcc gaaaaagacc aagttcacta ctatcgattc ttttatattg
    30481 atggttcctt caatgacaat ttagatggag taagtataga aggtttttgg tcaaaagcaa
    30541 aagcattttt cggatttggt agtgttaaca atgatccgaa ctttgacacc gttctcaaaa
    30601 aatctgaacc agttgatttt tttggaacag gtagccgtta ttttatcgca gtcttaaatc
    30661 ctctcagtgg aaataagcca agtggtgtat tactcgataa tcgaaaagga aatgtaaatg
    30721 gtgttacagc tctctatgag aatatttcca ttgaaccaaa tacttcggtt aactttgatt
    30781 atgctgctta tgtaggtatt cgtgaattag atggaatgtc attccgtgac aaaaccattg
    30841 atccaaaaga aaccaagtct tctcctttct cgggcctcag tgaaaaatta gacaaatcat
    30901 tcaatcaagg aattacgact ccattccgtc atggaattgt ttggatatta aagaaattac
    30961 acaatttaat tccaaattac ggatggtgta ttatcatctt tgcaattcta ttaaaacttg
    31021 ttttctatcc attaaaccaa aaacaagctg aaagtatgaa aaagatgcaa gagttaaatc
    31081 cacaaatcaa agcgataaac gaaaagtatg acaaagatcc cgcgatgaag caacaaaaga
    31141 taatggaact ctacaaaaaa aatggaacaa atcctatggg tggatgtctt cctatgttag
    31201 ttcagattcc aatctttatc gcactctata ctgcatttag tgatacaatt gatttatgga
    31261 aatcaccatt tttatgggta actgatttga gtgagccaga tacgatttgg acaagtccag
    31321 ctatcttagg aaccgcaggt atagcactca acgttcttcc acttatcatg gtaggaacac
    31381 aagtagtgca aacacaaatg acatcagtag caacggatcc aaaccaaaaa acaatgatgt
    31441 atttaatgcc actcatcatg ttgtatttct tctggacaat gccatctggg gttacacttt
    31501 actggactat ccaaaacgtc ctttctatct tacaacaatt tatcacgaac cgttttggaa
    31561 agaatacaaa accacagact gtgaccacaa ccacaacagt cagtaaaaag aaaaagtaat
    31621 aagaaattat aaggagcaaa tatgttagga tatatttttg aagcggaagg aaaaacaaaa
    31681 tcagaagcag aagaagtaac gttaagcact cttcgcctcg ctgaagggga tttaaaattt
    31741 gaaacagttg atcccggcaa gagcggtttc ttaggattta ccgccaaaaa gcccgctatc
    31801 gtgcgagcat atatttcctc aaaagactta ccagctgaaa aaattattca tggagttgtg
    31861 attaccatcc taaaaaaaat gggaatcgaa gcagaagtag tcggtatggg tgatgtggat
    31921 gggaagatat acatagaact agcaagtcca gaatctggaa tcctaatagg caagagaggg
    31981 gcaacactcg atgcacttca atttttatta aacctaatgg tagatccaaa aattcgtcat
    32041 ggtagaaaga ttattttaga tattgaatcc tacagagata aaagagaaat gtctctcgtt
    32101 cgtctcagtc gctccattgc atcaggtgtt gcaaaaactg gaaaatcaag actcctcgaa
    32161 ccaatgaatc catatgaaag aagaattatt catatggctc ttcaaaagga tgagaaagtt
    32221 ttcactagaa gcgagggaaa tggaacttac aaaagagttc gagttatttc cgcaaaagac
    32281 agacataaat ataaagactt agaaaccaaa aatccggaat taccggacga cgatgaatta
    32341 gacgtgtagt gattgataca atagccgcta tatcatcagg gaatattcca agtgcaattg
    32401 gaattattcg attatctgga gagaatgtat tgtcaatctg ttcaaaagtt ctctccggaa
    32461 aagatggtct tttaacttct gattttatcc aacaaaatcc aagaaaggct atctactgtg
    32521 atttcgtaga agatgatatt catattgatc aaatcgtttt tattttcttc aaaaatccaa
    32581 actcatatac tggggaagac ctagcagagt tctacttcca tggaaatcct atattattaa
    32641 aaaatgctct acaaattctt ttcaaaaaag gagcaagacc agccaaagaa ggagaattta
    32701 ccaagcgagc atttttgaat ggtaaattag atttaactgg agcagaagca atcggtagat
    32761 tgataaatgc tcgatctaaa tttgaattag agcttgctca aaaaaatgtt tttggtgaaa
    32821 ttcatagact tagctcaaga gttcgaagtg atttaattag tttaaaagct gaatgtgaag
    32881 cagaaataga tttttcaaca gaagacctaa cgtatgaaag tttcgaacaa agaaaagaaa
    32941 gaattcactc tctttataaa ctttgcacaa aagttttaga tagctccaag agagcagaaa
    33001 gttttataga acacaataaa attgttatct atggagagcc aaatacagga aagtctagtc
    33061 tccttaacag tctaattggt aaagagagag ctatcgtttc tagcattcct ggaacaacta
    33121 gagattacct tgcagaaaat ttttcgctca atgggattcc tttacaatta gtggacactg
    33181 ctggaattcg agaaacggaa gactccatag aaaaaatggg aattcaaaaa agtcatacag
    33241 aattcgaaag tgctaatttg cgacttttta ttttagatat atccaaacca attctattcg
    33301 atgaattgat agaacgctac gaacataaaa ttcgcaattc tattttggtt gcaaataaac
    33361 tagacattgc ccatgaatct tgggttaaga ataaagaatt gtttcgatcc gcgaatatag
    33421 aagcctgtga agtttcctgc aaaacaaagc aaggtatcga tgaactttta aattcaattc
    33481 attctcgttt aatccaaata gacaaatcag acgactatgt acttttggaa gaaaggaatg
    33541 aattttactt ccgaaaaatc caagaacatc taaaaaatgc aatcgattta atggaatcag
    33601 ctccagcaga aatttacatt aaagaaatag attatgcatt ggaaaatgtt ggatatataa
    33661 acggaaaaat tgacacagaa gaaatactcg gaagaatttt tagtaaattc tgcgtaggca
    33721 aataaggaaa ataaaatgga agaagaagat tacagggaaa aagctttaga atatcacaga
    33781 gcgcatccgc gcggcaaaac aggaatagtc tctacaaagg ctacagaaaa tgcatatgat
    33841 ctagcccttg cctactctcc tggtgtcgcc tatccttgtt tagaaattca tagcaatcca
    33901 gatcttgcat acgaatacac aaatagagga aatctacttg gaattatttc aaatggaact
    33961 tctattctag ggcttggaga tataggagct atcgctggta aacctgttat ggaaggtaaa
    34021 tcagttttat ttaaaaattt tgccggtatc gatgtatttg atatagaact caatacaaaa
    34081 gatgcagacg aatttattag agccgtcaag ctaatggaac caacgttcgg aggaatcaat
    34141 ctagaagata ttaaagctcc agaatgtttt tatattgaag aaaaattaat agagatcatg
    34201 gatattccaa tcttccatga tgaccaacat ggaacagcaa tcatcacagc agctgcaatt
    34261 atcaattatc ttattctatc aaacaaaaaa gcagaaaatg taattgttgt tgttaacgga
    34321 gctggagctg cggcgattgc aattgcaaaa ttaattattc atctaggagt tgagtctgaa
    34381 aatatatttc ttgtagattc aaaagggctc atcacaacta gacgttccga tttaaatgat
    34441 atgaaaaagc catttgctcg aaatacaaat gcaaatacct tagaagatat catgaaaaac
    34501 gctgacatat tcatcggtgt ttccaaaaaa gatcaagtga caagagctat ggtaaaatct
    34561 atggcaaaaa atcctctcat acttgcaatg gcaaatcctg atccagagat ttcatatccc
    34621 gatgcaatct ccgtaagaga tgatttaatt atgggaacag gaagaagcga ttatcccaac
    34681 caagttaata atttattagg atttccttat atattccgcg gagcgttaga catgcgggca
    34741 agaagaatca ctctagaaat gaaacttgcc gcgacctatg cattggctga acttgcaaga
    34801 caagaagttc cttcttatgt atcagatgct tatggtggag agaaatttca atttgggaaa
    34861 aaatacatta ttccaaaacc ctttgatcct cgtgtatatt acagcgtatc atctgctgtc
    34921 gcagaagctg cacacgcaag tggtgtagca cggattccat atccaggaca tgccgcctac
    34981 cgatctcatt tagaaaagta tagagttcga aagatttagt atgccaatca ttgaagaaga
    35041 aacagaagtc agcactcgaa ttgataatat ctatgaacac aaagtcattt tacacaatga
    35101 tgatgtgaat tcttttgagc atgtagaaga ctgccttatg cgtatttgct tcaaaacaaa
    35161 aaaggatgcg gaaaaaattg ctttagaagc acataacaaa gggaaagccg tttgttatga
    35221 aggtagcatc gaagcctgtg aaactgttgc tgaaaaaatg ggtgatgaac gtttaactgt
    35281 tagtctagct taaggattga tttatgaata aaacaattgc ttttattggt gttgggatta
    35341 tgggtcgtgg tcttgttaaa aacctcaaaa aaacaaatac aaaattaaag atctatgcgc
    35401 gcaatcaatc aaagatagaa tttctaaaag atacaaatac agaaattttc ccaacgatag
    35461 ccgacacggt aaaagatgct gatttcatca tactctgtct cacagaggat gaagttgtcg
    35521 agaaagcttt ttttgaagct gtggctgaac cacaaaaagc aattgtttta gattttggaa
    35581 caagttctcc aaagctgaca gaaaaaatgc aatatgagtc aaaaaaaaga ggctttcgtt
    35641 ttatagattc tcctatgaca ggaagtaagc tagcagccga taaaggcgaa attttattta
    35701 tggtaggtgc tagccaagaa gactttcaag cttgcaattt tatctgggaa gcttgtggta
    35761 aaaaagtaat tcattgtgga gatgttggaa atgggcagct aatgaagatt accctcaata
    35821 tgatacaagc gggactagca caagtttata tggaaggtct tatacttggt aaaaagctaa
    35881 gtttagattt gggagtattg catgagatta ttacaaattc tgctgcacgc tctggaatat
    35941 ctgatttcaa agtaaattgc atactaaacg atgactactc aactcatttt tcattaaaaa
    36001 atatgaataa agatttaaat catgctcttc gtattgcaac ggaaaataac gttagtctac
    36061 caattattca tagtgtaaaa tctatatatg atgcgggaat gtctcataat cagggagagg
    36121 aagattttat aagtcttttc aaaataaata aacaaatcaa taatattaat taatcttata
    36181 accccatttt aaaattaagt cttcaatttt ttcttttgta aatggcttgc ttgcataatc
    36241 attcatacca accttaaaac aagtgtctct agtggaatcg gttacacctg cagtcactgc
    36301 aataattgga gtctccttgt agttttccaa ttctctaatc tttcttgtcg catcatatcc
    36361 atccatttct ggcatctgaa tatccaagaa tattaaatta aattgattat tttgacaaag
    36421 ggagacagcc ttttttccat tgctgacttc tgagagcttt gctttcggta aaattctaga
    36481 aataatggtt ctaatcaaca ttaggtttat ttcatcatca tctgctacta gaatttgaat
    36541 tggtttttct tttaaaatgg caaggctcgg taaaatctta cttgcctcct ccggttctcc
    36601 aatcttaact cttaacttaa aaaagaactc actgcctttc cctaactcac tattaatctc
    36661 taaatgacta tccattaatt ggagtatctt atttgttatc ggtaaaccaa gaccgatgcc
    36721 accatactgc cgaacattgg attgatccac ttgagaaaag aaatttataa tctgagtttg
    36781 gttctcttta gaaatgccaa tacctgtatc cctaatggaa aaaagaaggg tcgcttcttt
    36841 atccatcggt tccattgcgt catgcgttac ttcaattact acatctccac tgtctgtgaa
    36901 ctttatagca ttacttaaga gatgtatcaa aatttgtctc aagcgaattg aatctgtata
    36961 aatataatga ggggtttttt ctgaaatttt tagttttaat tttatatttt tctttaactt
    37021 taatttatga atctcagtta cttgatctag aacactaatc aaatcaacct tttccatttg
    37081 aagttgtatt tttcctaaat agagctgacc aaaatctaag agatcattta actcgactaa
    37141 caataaattt cccgattgaa gaatcgtatt aacatatttg atttgaattt catttaaatg
    37201 agtggatttt aataaatcag aaaaacctaa aatcgcattg agtggcgtcc gaatctcatg
    37261 actcatattg gaaataaatt gagattttgc tgcgttagcc gactctgctt cttcttttgc
    37321 tcttcttaat tctatttctg atttcctttc ctcagtaatg tctttgccat agcaagcaag
    37381 tccaattatt ttcttttctt tataaattgg ttttataatt aaactaatcc atccaaccgg
    37441 accttccgaa tctaatgtcc ggaattctgt tttaacaatt tcaccgctaa aaccttgatc
    37501 ataaagctta cgccaatact ctaaaatttc ttttgtgtaa taactttgat ccaaaagaga
    37561 atttcctatt tctaagattc ttccagtaga tttttgtata cttgaaataa atgcgttatt
    37621 tgctgtaact aaacaataat ttctatcaac cccccaaatt aaatcatcta tattattgaa
    37681 cagagcatct ctttcaatat tcagattgat aattgtattt tctttttccc ttctctcgta
    37741 aagagttgcc gttaatacca ttgtattaac cataacaatg ctaataaagc tgtatagaag
    37801 aagtaaactt gaattgattt cgcctgtata aaacggtccc ttaccgttta ctgttccaag
    37861 aaccgcgatc atcattgcaa taaatgcaaa taaataagag acttttgctt ctaagcgaat
    37921 cgctgaccaa attaaaaatg gaaatacaaa ccaagtcaga tattttccag aaatataaaa
    37981 atcatattcg aaatgaatca tgaattgtat aaaacataaa aaggaaaaca aaagtattgc
    38041 ttctaaaaat cgcttaacac tatgaggttt ggtagatata aaagaccatg ataagagaag
    38101 agatcctagt gtcagaattc caaatgcatc ccccgcccac caagaagcaa ttgcataacc
    38161 aatattactc catttcaaca tcccattcaa gcataatcct attgaaccaa taatggagct
    38221 gagaattgga gaaactacta ctgaaaataa taaaaagaag aaaatatctt ttagcctaag
    38281 tagagatggt cttattttaa attttgtttt cgctaaatag attgaaataa ttggctcaag
    38341 agtatttgcg atagtcgcaa ataaaacaaa gctaattgga gcatttgttg ttaggttggc
    38401 gacaaaggct ccaactgcta ttcctaaggt aaatctatat ccaaaaagaa ataaaatggc
    38461 taccgagaaa ccggaaggta accaaaatag agttacatta gattgaataa atgcaaatgt
    38521 taatccaatt tttgctaata caaaaaaaag gatggcaaca agagcattct taaaaaaata
    38581 attcaacttt ttcatttgat aatatttatc aaaaaaatac aacctagaaa ttgaatcgat
    38641 agttttttaa ttgcaccaac ttttatcacc acttgcaatt gctttacact gtgcagagct
    38701 acaccaagat ttatctttag aagcaactcc tttgcaatca gaatcattgc accaactttt
    38761 atcactactt gcccatgctt tgcaaagagg agaactacac catgacttat ctttagatgc
    38821 aactccttta caatcatttg tctgacacca actcttgtct ccactagcaa tgcctttgca
    38881 atcgttagtt gtgcaccaac ttttgtcttt gcttgctatg cctttacaat ctcccgaaga
    38941 cagggaagag atgaaattac ttccccaatc agcagcggcg aggttaatag agattaaccc
    39001 cataaacaaa attaacttaa ttttattcat ctatattctc cttttatttt aaaataagct
    39061 caatgttttt atttactatc gtctttttgt tttattggaa gttttcctaa aacatcattt
    39121 ccaatgttta gaaatactcc ttccaattta gttccagata aaagtctagc aaatacatct
    39181 gctaccgact ctcctcccaa aattgctaga ggagccattg cttctgcaat tttaacaagc
    39241 tgctctttgt cattaaatga ctgtaatgct gcaataaaat cgggactaat tgctttggct
    39301 ctttctacca tagccttaac ctgtgtttct aattctttag aagatagatt gatattctgc
    39361 tctttgaaaa acacctcttg ttctaatcta gatttatctc tatctaattg cttctcattg
    39421 atttgattca ttaaatcttc tttgcgtaat tgattactta attttaagtc ctctataaat
    39481 gcatttgact tagcacgtgt tgtctccaat tctaaacgtt tttctaattc ttctttttca
    39541 aatccataga cagccttttg agtagtatac tgagattcac ttgtttcctg cttaaccttt
    39601 tccataaatt tcagaatttc taacttagac ttttcccttg ttagagtggt agactcttgg
    39661 atcagtgctt gttgtgattc catcattaac tttgctatag tcgcatcttt gattgcaata
    39721 tttgtaactt ccacatcata gactctcatc tcattttctg agaaatggta accactatta
    39781 tcctctccaa gaattgtttt tttgatgata ttaattccat tcgtataaaa ttcttctact
    39841 ctgtaatttt taacagcagt gcggataacg gacctaaacc gatcagttaa aaattttaca
    39901 taattttcta cattaaacca tttagaggaa tcattcacaa agtttaatcg atacgaaagc
    39961 ttgatttgaa ttccaacaaa atccttcgat tctacatcga ttgtatcaga aactttattg
    40021 tttgctacac ggagatacac atctcgaact agattttcat cggatttagg atttccctta
    40081 gaaagagaga aaacttgtag atcttcatca aactctaaaa gataagtgct aggaccggcg
    40141 atgacttttc tctctccaga ttttcctaca accataattg catatcccgc ccaaactcca
    40201 atggatactg ctccgtcata ctttgtatct aacgttatat atctaggcgg agtatattta
    40261 tttcttcttt gaaatgaatc actggcaatt tgctctttct cctccatttc ctcaaaagaa
    40321 ggagccagca attcatccga agcaaacgct ggagctggag ctgcttgagc acgaagacta
    40381 ctagaagctt tatcagcttg aattgctttc tttctaagtt ccgcatttac ttcttgagct
    40441 ctgtcatttc ctggaaacca aagaccaacc tgtcttggtt ctagtattct tcttacaatg
    40501 acttcaaatc gtggatcagg aagaaacata tctggacctc ttctaagttt tacttcccct
    40561 gttaaacgat tcattacata acgagcttcc cctagaggaa ttgcgactgc ataatgaact
    40621 tcattgtctt catattttac aattgcatgt tcttttcttg ggaaataaat catttgatct
    40681 cttcccgtaa taaacaattc atctccctct ttaaactgtt tataatttga actaccaacc
    40741 tcgccttctg tataaggtgc tatcactttt atatatatac ctttgatttc actcagttca
    40801 attgctctat actttctact tccatctttt tgaataaacg tttctgttgg ttcagggaaa
    40861 actacctcag gacctctcac ataacgctta tttccatctt catctagaag tatacaatat
    40921 tcaagtcttt cgagtgttac tgcttctcta atatactgat tatttttgtc ttttacaact
    40981 tctataccgg taggcggtat atagaaagaa acctcagttc cctttatgat aagatgtttt
    41041 cctaatttta attttgatag atcaaattct tgttcgcctg tgctagattc acttttcttt
    41101 ataacggctt tgttccaatt ttcctttgca gcagtttcat cataaactct tacaaccaag
    41161 tattgattgg atctaaggtg atgacctttt acaacgttaa ccatttgacc agaccaaagg
    41221 ggaaaggata ccggtcctgg tatatttact ttgtgaccaa cattgagttc agtcaggctg
    41281 tttactgttc ctgtttttgg atttgaattg tccttagctg ggttttttaa aacaagatac
    41341 cacccttcag gagagatggc aaaagtttga atggaagatt caagggaaca tttttcaaaa
    41401 cgcttagttt tttcattaaa aataatcggt tgatctgtat tggaaagact tgctttataa
    41461 ggtcctacat agatatttat atttcctttg gtttgatc
//
LOCUS       DAOQOS010000042        41433 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_381_length_41433_cov_5.251220, whole genome shotgun sequence.
ACCESSION   DAOQOS010000042 DAOQOS010000000
VERSION     DAOQOS010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 41433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 41433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41433
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_381_length_41433_cov_5.251220"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..674)
                     /locus_tag="PLF66_11080"
     CDS             complement(<1..674)
                     /locus_tag="PLF66_11080"
                     /inference="COORDINATES: protein motif:HMM:NF016827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-54 factor"
                     /protein_id="HMZ64630.1"
                     /translation="MLGQHLVQKQTQKLVMTADLRQSIELLPLSTLELADKIQAELID
                     NPLLEEVFNPEKPKTPEIYSVTEIREKEKRELTKSTEGNLTDIYSIEGPYRYDNEARD
                     KNQSMIEASPIKEKLSDHLLSQLRLLDVTDEELEIGEILISMLDEHGFITTPLDEIAK
                     EMNLNFKLVKKVIQNIHQLDPVGIGASSIQETLLIQAQILRPEDTNLAILLREHFKDL
                     EKLDYK"
     gene            complement(674..1381)
                     /gene="lptB"
                     /locus_tag="PLF66_11085"
     CDS             complement(674..1381)
                     /gene="lptB"
                     /locus_tag="PLF66_11085"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004430733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HMZ64631.1"
                     /translation="MENLVKIYNKRKVVDGVSFKISKGEIVGLLGPNGAGKTTSFYMS
                     VGFVRPDSGHVFIDDHDVTQAPMYQRARLGIGYLAQEASIFRKLTVAENVEAVLQTLN
                     LSRKEIQERRDELLLELQIMRVANQKGFTLSGGERRRCEIARALATKPDFILLDEPFA
                     GVDPIAVKDIQTVIKSLRERGLGILITDHNVRETLKITDRAYIMYSGKILISGVTQEL
                     VNDPETRRIYLGEDFTL"
     gene            complement(1399..2805)
                     /locus_tag="PLF66_11090"
     CDS             complement(1399..2805)
                     /locus_tag="PLF66_11090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64632.1"
                     /translation="MKTQKGNKLNLPFIWIIFTFSGLLSIQANTRPALLISPLNLSDG
                     NFPYIETAKSSKKNQSKIPATWGGATLSQETKNVEGIDMTVYSLTGGAWILHKKVKLS
                     ANSIEIVGEDAYRGHLKGQTKVEDPENGITLTAGKGIYDKAQETVILEGRPTLIFVDK
                     EKKVTKITAPYLKRYLSDNKTVFEGGAIVENSDYTIYSDAAIFEEKEESLTMEGYPFI
                     FGKETFLTGEKVTYSNSTKNTVLENDTILLKLSFENPKKKTIRKDSKEADNTDKPEET
                     EKIKKISIFTGDKLINQSGDEETRFIGMFGNAKMTRDDFEFTGSYIKAFGKENGNIEA
                     KEGVTLLDKENHVRLSGDTLEHSKEKDYSHMTDNAMIEFLDKENENVNSSMTAIEVER
                     FGDKKEIVCRGDVNLVSSDSTIHGEYATYFEESEKMYVEGNPSLKKEEKTVYCGRIII
                     YPNSDRIILSDGLNVNKK"
     gene            3131..5392
                     /locus_tag="PLF66_11095"
     CDS             3131..5392
                     /locus_tag="PLF66_11095"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HMZ64633.1"
                     /translation="MLKKKINIPNLSFEEMFFNSDAIHLLIDPETSEIICANPAAERF
                     YGWSKAELVGKKIQDINTLSPEEIKNALDQVKNNRQGFFEYKHRTSNSIRDVCVFSNV
                     IDHNGQILLHSLVYDITEQKRKEETIQKQETFLRQVETLAKIGRWEVDILNQKVFWSK
                     GMFDIYELEEKKESLNFDYGLNLVHPDDRDILVNAFHHSLREKIPITMEHRILLKNGK
                     IKTVNETFKTDFDLKGNPIRTIGVLQDITDRIKTEEALRLSETTMKAIFDNSYESFIF
                     ISEQKQILFFNKQAQDRTKRTFDVTLQVGDSIYKIVQPDSLNLFNSNFERAWNGERVV
                     YDKHFTINNLIVWYEFQFIPVKNEKRNTIGVVLTARDITEKKKAELDLKISEARFHAI
                     FDQAPLGIALIESKSGKFIQINPKSTEIIGYTEDELLKMDYQRISYPDDLKEDLENMD
                     KLIQGSIQWFTMEKRLIRKDNRVIWTSLTCVPLKLEEDNFTYHIVIITDITERKMANL
                     QLEDFTESLKKLNATKDKFFSIIAHDLRNPFIGILNLIKVILLNIDKTDKFHENKKYI
                     ELIQDSSRSAYDLLENLLHWSRAQTNDIDLFPETIYLKKLVLEVVNLFKGNAAAKNIN
                     IEIEISDNVFFFGDEYLTSTIIRNILSNALKYTYRNGRINIFCRDFTDRIEILIQDNG
                     IGISEENLNRLFKIDEKFTESGTEQEQGSGLGLILRKEFIDKQSGLIEVNSKLGEGST
                     FVITLPKQSAFDL"
     gene            5545..7479
                     /locus_tag="PLF66_11100"
     CDS             5545..7479
                     /locus_tag="PLF66_11100"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HMZ64634.1"
                     /translation="MQHYSFTDANLPINQIIDTLPIGFVYQNAEGEILYANLAAQRIL
                     GLSFSQINGRNSNHPDWMSIHEDGSFFSGDEHPGPMALRTGNSISNVTMGIFNPIKQQ
                     YIWIQIDAVPVFIENSQKPIGVYAVFEDITEYKKNKDVEKDLFVKLRKSNLEVSQRQQ
                     AIDQHAIVAITDYNGTIIYANSKFCTLSKYSREELIGQNHRILNSGYHSKYFFHNMYS
                     TIKKGETWHGEIRNQAKDGSYYWVATTIAPLRNLDGEIEHFIAIRTDITKRVQAEEAL
                     RISEARTRAILDTSPDSIIFIDLDYRIQFFNDTANSRSKIFLNTELTIGKSMLHILPE
                     SRIENFTLSFHSALKGEIITHIRNFSTNSGTVWFEVLYTPIKNKSSEVIGVMFTARDI
                     TAKREAETERERYLEDLENLNHTKDKFFNIIAHDLKNPFAGILGISEMLESKLKESES
                     QYTKEDLLKMANLIQSSARSASNLLDNLLHWARSQTGELQCDLIITSINSIIEATIPL
                     ISGIAFNKQIEIEKNLDAEKFVLADPSLTNTILRNLFTNSIKFTNPGGKIIVRTKFNN
                     DYAEISIEDTGIGIKEEHLSKLFRIDSKITKLGTNGEKGTGLGLILCKEFTEKQNGKI
                     WVESEFNKGSKFTFILPIAL"
     gene            7504..9003
                     /locus_tag="PLF66_11105"
     CDS             7504..9003
                     /locus_tag="PLF66_11105"
                     /inference="COORDINATES: protein motif:HMM:NF013179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HMZ64635.1"
                     /translation="MNSSSFAQAALDAVSANVCVLDKNGYIIAVNQGWREFFEENSNS
                     SENKSIGLNYLDICSTSSGARSEEALPMFNGIMSVIKDEAKEFTLVYPCHSPTEQRWF
                     RARVTKFNDGSGNIVIAHELITERKLAMDALRLSEARMRAILNNVNESFLFIDLNYKI
                     QFFNSLALKRIYHMFNITIQEGENFLDKLIETDRYMMSTYFNLCKKGEAVEIKKIYKV
                     NSYPRWYDIKFIPVKEEDGEIIGILFTTRDIHRQKKIEHELVRKSENLKELNATKDKF
                     FKILAHDLRNPFFGISGVTEILKERLSNIPNYDTAELQKMVNLIQASTGSASALLENL
                     MEWAKAQTGSLQIDLVEFNINKEIEKVIDLIKGSAFHKNITIEFKLENVNSVLADSYL
                     TNTILRNLLSNSIKFTPIGGNIKIVVKQTGKNVEIAITDTGIGIPEESIKKLFRIDSK
                     FTMLGTEKEKGTGLGLIICKEFAERQNGFIRVQSAVGEGSTFTLCLPHI"
     gene            complement(9080..9298)
                     /locus_tag="PLF66_11110"
     CDS             complement(9080..9298)
                     /locus_tag="PLF66_11110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003005646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HMZ64636.1"
                     /translation="MNFETFRNKLGKKIKEERLARGFTQEDMDEGDDGVPHRTMQNIE
                     TGKSNPNLKTLFRLSKRLNISVSELTDV"
     gene            complement(9662..10237)
                     /locus_tag="PLF66_11115"
     CDS             complement(9662..10237)
                     /locus_tag="PLF66_11115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020768249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HMZ64637.1"
                     /translation="MISTIAVFPLPNMVFFPGAFLPLHIFEPRYRLMLDYCIESDYEM
                     AVTALKSDSEIETTFGWGKIVRHEPLKDGRANILLEGMGIAILKKFNSTEPFIIAEVD
                     KIDNDYSHINTEEFKELTNELIRLTRSYLMKLNVENVYINEIQKLTSHPFPVEFITSV
                     INCDYKRKQEILITENPYQKAVKLIQILKEI"
     gene            10352..11827
                     /locus_tag="PLF66_11120"
     CDS             10352..11827
                     /locus_tag="PLF66_11120"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HMZ64638.1"
                     /translation="MAKKIAKKKETVKIQKPEKKTDEFRKEVDRLFQLQQANRWNIAN
                     TTANQRIEKLNKLRSVIEKYSQEIRDALHSDFRKSPQEVDLTEIMPVISEIKDAVLHL
                     KSWMRPLRVGTPLSLFPSSSQIIYEPKGLILIISPWNYPFHLIGTPLVAAIAAGNCVI
                     IKPSNNTKHTAAITKKIVSEIFNENEVAVASSDRDTAAALLEKPFDHIFFTGSTSVGT
                     TIMEAAAKNLTSVTLELGGKSPVIVDESVDMDDTTAKVIWGKVLNGGQTCVGVDYVLI
                     HQSKYNEFIESCKTQVKKFYGQTPEDWQKTPDLCRIINDKNFERLEDIVKNSIKQGAK
                     LELGGDFKKEERYISPTILSEVTLDMPVMQEEIFGPILPIIPFNTLDEAIAIIRSKHK
                     PLSLYIFSEKDATIEKILKNTSSGGVVINGVVAHLGNPELPFGGVNHSGIGNYHGHFG
                     FKAFSHERAVLKVSKLTLLKMLFPPYTKTTKTFVDFMTNYL"
     gene            11886..13151
                     /locus_tag="PLF66_11125"
     CDS             11886..13151
                     /locus_tag="PLF66_11125"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001190629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HMZ64639.1"
                     /translation="MEIERLKFLTEEETRSIAKEFGTPVFVYSQKEIESRCDIALAFP
                     NEYGLTVRYAMKANPNNAILKIMKKKGIHIDASSGFEVERALLAGFKPSEIMLTSQEL
                     PSNLKEIVERGTMFNACSLHQLESFGKLFPNQEVSVRINPGLGSGATQKTDVGGTTSA
                     FGIWHEYIEDVKKIKEKYGLKITKVHTHIGSGSDPEVWKAVSKYTLLYAEIFTEATTV
                     NLGGGFKVGRMLEEKTTDLQKIGQPVKELFIEFNQKHGRKLKLEIEPGTHMIALCGSI
                     ITKVMDKVDTGKNGFEFIKLDTGMDSNTRPSLYGSRHPLITVTANGSLPQKTKNYVVV
                     GHCCESGDLFTQKEGGSPETREMMEANIGDLVVMEGTGAYCSGMSTKNYNSYPETPEV
                     LIKSDGQKVLIRKKQELKQIFENEIEIAV"
     gene            complement(13152..14855)
                     /gene="recN"
                     /locus_tag="PLF66_11130"
     CDS             complement(13152..14855)
                     /gene="recN"
                     /locus_tag="PLF66_11130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004760665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HMZ64640.1"
                     /translation="MLKLIKIKDFALIESLELELDKEMVAITGETGVGKSLLLDAISS
                     LMGSKCNTMNIRSGAKKYQLEAIYDISKNPKAIEWLNEKGIEHDGLEIYLKKELFSDG
                     KSRIQIGGSLAPSQFLKELGSLLSEIHRQNDQLFLLEKSMQLEYLDGYAGLTSLKKEV
                     SERFKTYQTLKNRISEIDKMSSDKNHRIEILKYQIKEIQSAKLQLGEEEELLQDEQML
                     IHGEKIVENYNLIADLLNEGEENILKSFSKILNAADKIETVTKDFSTNRNQLYEIYEQ
                     LKDISHTVIDQKDEIFFSSDRLSIVQNRLDEINKLKRKYGKDISAILDFLEKAKIELE
                     SLEISSDEVISLQKELDSMRDNLTNLAVKLSHLRRDSILKLESDLQKEFQLLGMKDAK
                     LQIVMRWEQAIDGEVTDGGKKYILNENGLDQIDFYFSANQGEKPRPLRKIISGGEMSR
                     VMLAMKTILGNNNEPRLLVFDEIDAGIGGEVAYRVADKLKSISEKNQVLLITHLQQIA
                     AVSDCHIKVEKDIMNGRTFTIARKISDSERAKELAKMIAGEHITKGALDHAKELLKKA
                     V"
     gene            15598..17457
                     /locus_tag="PLF66_11135"
     CDS             15598..17457
                     /locus_tag="PLF66_11135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016546991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HMZ64641.1"
                     /translation="MIELKYGTRKVISFRGAKKVVGGLSDQNKIAVLLYISKEFSNVG
                     REEDLFDTLITLCKEIFECDNTTLRLWDGELLVPIKYIKETTPPRRNLTPSEGYSGQT
                     FSNKKPLLIPDLKKNPQFLDDQETTQCVICVPITYKDDVLGTISVESDTAFFYKEDDL
                     EILEALGAQLALALTGVRLIEGLMTARAREAAILTQLEWDLKMGRNVQSQILQNQLPP
                     WNGIYFGTHYEPMVEVSGDYFDVVKQGNTMTAIIVDVSGHGIPAALVTMAIHFHFNRF
                     VTQGLGLAEIMEQMGEALKPQLPESTYFTAFIVRLFHDYTYAYINGGHQKVIHFKNDA
                     TIDDLDSLGVPLGILDVKKSDYEEMHGKLEPGDYLLMFTDGFTEQKNSDGEEYGQTRF
                     KESFLRARDLLKTEKQNVFVKDIVKSMLSDWNQFKGSQKNGDDLAMLLLQCNTTLTDA
                     LPLVRMAKVSAREKNNADAYSLALQAYRIDPSIKDNLLFLGKMYYNDGKYEESVKFID
                     EYIKTSGEDTAIIHYLYGKALFNSNRIAESKRALKKSLSCDHTFAKSSLLLAKCYLKE
                     NAIPKAIKTLQRGIKSTPSNDALKVSLKKLEGINIIRTANTIEVEEDNSGEEQ"
     gene            17529..18890
                     /locus_tag="PLF66_11140"
     CDS             17529..18890
                     /locus_tag="PLF66_11140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010575737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HMZ64642.1"
                     /translation="MPLSPEVIEINTIKADLSQFKGVMESIQKESNSLKAEINLLWIC
                     IAAFLVFFMQAGFALVEAGFTRAKNTVNILMKNLSDFTIGSICFWLIGFSLMFGPHLL
                     EGIGIGKISFLETSLLLEDGKPSPFKYGFFLFQLVFAATATTIVSGAMAERTKFTAYL
                     IYSFLMTALIYPIFGSFAWSNLFDTNNPGFLAKMGFVDFAGSTVVHSIGGWAGLAGTM
                     VLKPRLGRYQPGGVIIPILGHNMTIAAIGVFILWLGWFGFNPGSSGTIQGGSFAIIAI
                     TTNMAAAAGAISAMTLSWILFTKPDIGITLNGGLAGLVAITAGCHTVGITSAVIIGMM
                     SGLLVVGGVLFLDKLGIDDPVGAVSVHGLNGAWGTLAVGLFADSTFGGGPNGLFFGGG
                     FSLLLTQIIGVLVAFVWSFSLSYLVFFTMEKTIGLRVTEDEEIIGLDLLEHGNEAYPD
                     YKS"
     gene            complement(18887..19504)
                     /locus_tag="PLF66_11145"
     CDS             complement(18887..19504)
                     /locus_tag="PLF66_11145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002972414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64643.1"
                     /translation="MTKKITFILILFLFPIFAEPIQEKKKINLIVYVGKYTSTDLLPI
                     LFRQKTDYRESYIGVLGINYPMNYRVRFFEIESEALVGKHFGIMNHWEADGLMIARVS
                     NLFGLPMSFAFGEGLSIGSQNPILENQSKGFNLKNGQIERQSIESRNILNYLMVELDY
                     RIWDLPSNPRIFMRVHHRSGIFGFYCPPDPACGSNFISYGFKMSY"
     gene            complement(19501..20151)
                     /locus_tag="PLF66_11150"
     CDS             complement(19501..20151)
                     /locus_tag="PLF66_11150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64644.1"
                     /translation="MTKKLFLILIFVLIHCAYETAQRTTLDSKEIGSRKVLIQSEDKI
                     LTHILCDSVLKSGEGQCISEPSLLDKDSILISVSAKIGDSSSDYFINVGKSIATLTLG
                     VFLSNSATVHFNVKNLNQESEPLKIKSEGRIGRWAILPFYAGLAATSFGGILNTYRNP
                     DHLQKYCLTEVVSRTRKILEASKEEYCADYLSYLQDSFNKVEINFIETIRFIGKNQ"
     gene            20263..21489
                     /locus_tag="PLF66_11155"
     CDS             20263..21489
                     /locus_tag="PLF66_11155"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HMZ64645.1"
                     /translation="MSTKSIIQYTKGAMIAMANTPNPGYVHILKSGEIAIESIFKFSS
                     KDLNRYLPGDTFGYVSAITKNPHSTTLVAVTDCIVIRLSVENFFEYLKNNPEVFLKII
                     SYNAEKLRAFVNHINPYQKDQTPTTDSPEKLIHNAKIYLEHNDINLACFSLKKYLDGK
                     FEREKETEKIKEAEDLLQKTNPKYTLPDYPNYSDDTDFVLKKGDIVFVEDEAEDDYFY
                     IVKQGSIKISKIVEGREFILGILGKGEIFGEMAILNQRSRNATAIAFEDCVIQRLTAN
                     TILEKVDETILTKIFHIISRRLWYAFQRVFMLKVSDPNVKLYIQLQMLIADEMSKSEV
                     DRSKEKFVFRFSLQELLKMVDILDISSDRISEFLSDSNLSFSHGLVVVKDKQMLDSKV
                     DTVKKRHTRLLKEIII"
     gene            21544..23799
                     /locus_tag="PLF66_11160"
     CDS             21544..23799
                     /locus_tag="PLF66_11160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64646.1"
                     /translation="MENKSILTDPKISAQQDSKSKEDSAAYIHMPTYSKITNLLKRIL
                     DLARDRHSQIKSLNGSESDVSSLLSLDYLHTLLQKETSLKETEKTPASINGALKQMLD
                     SYTNGKEKYGYLYPILITEGNKIIPKIILIAPQKEKQTPIQPYRESCFQFSKKIIDQM
                     LVTRAKNTRRSDDTTDTSLLRLSKSGSEWLFPKFLSVESELVSLLKKGFQPYAYIPLN
                     DFIEDFTNYGLKSDKLVQILPDYHMIQEKVEFTDNGEFAKVPEVIYHYKSQVESLEKF
                     VIKYLGSSAEKLKFNLYQTSIQQFREKYNTSYPDDPTTIKERMDALFDLISLFPGTGA
                     QDENIKKILETCDESIQICKKLYARMDDLVRYKEKHLSTVFLNSVQKKIDDHTKEKMT
                     LYILDLKTTLNKSNIQDKQLLYELEKELKQIIQTKYGYFESMSNEGREFFYVTHPGYM
                     TCTIHNLTRLTIENKSYQKQLDIAKIIANKLEEARRTDIDSLVTEEDKQRLLKKIEEI
                     RSQETQRLKRLEFRNNYNIFAGVAGFIISLIYFLILYNTYNDLIYALLSIPVSVFTGL
                     LFSVLFRKKPFIVRNEKELEENESSESSAGSETEKTNQIIKASESYVFKSKFNSIDER
                     IHDRNSLKETIKANLDIIKANSQIFAKEKNDEKIISTIENAMLFYSAVITVPSDITPK
                     GKPTMIILQRSDLKNPHIRSQIAEYFKFQSAAGRSTGSTAYYNYLYQAVERDYFKYVK
                     GSNIQRLKKEL"
     gene            complement(23794..24810)
                     /locus_tag="PLF66_11165"
     CDS             complement(23794..24810)
                     /locus_tag="PLF66_11165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64647.1"
                     /translation="MKSIFYLLFIILFDIQCNIGSAQNNSYADVLLKHPATDLNNYNQ
                     KGVWEDRIYNIDELRIDYVKQINLIDGFSEIPQPTKSMNQMKSLILQIAKNLNPQVKP
                     LFEKYIYGIYFCEKLGGTGLTGYIYNNEENKPIGGIIIIDSNVIQQKANDWITFKENS
                     VFDKSNTELKIQIEDDKNNTLENALRYILLHEIGHILSNVYRITPDARNKTLDYSNHS
                     FLNKIWKTETDSYYDSSEFPFRKNISFYAAPDKKINLDTDWKKIYPVLLKYRFPTMYS
                     ATNASDYFAEMFVSYVHCILDKKPWKLSIYQSNKEIYSIENGIHFLHEEREFIRKSIF
                     LKEL"
     gene            complement(24930..26045)
                     /locus_tag="PLF66_11170"
     CDS             complement(24930..26045)
                     /locus_tag="PLF66_11170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000431183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HMZ64648.1"
                     /translation="MNEIDVELNTEDQSFNKIKTLAKEAYKLIDQRKYKDAKEKFTEL
                     LVTDPNNTYGLVGMGDLLYKTQNYEEAIKYYRNCLNLYTSNKFSIMGLMNCYRDMKQL
                     NQVIQIAEEYRHITMSDASILSRVADAHRKLKNFKESEIYYMSALQINPTDQYVIVGI
                     GHLYFACQRYQDAIKWWEKLVATQPNNIKILTEIGNSYRKIKDFDRAIEYYERARVLD
                     ETNFFALYGLAESYRGKKEFKKAIIYWEKILHFDPNNKLIVNRYADSLRGLGEYEKAL
                     ECFNRILNSDEDYFALLGKASTLNLMGETEKSQEIFLLLKRKDPLNPRPVIELADLWF
                     YSKNEKEKAISILKDFLKSNPSNTDVSMKLDQLQNEA"
     gene            26233..26997
                     /gene="lenA"
                     /locus_tag="PLF66_11175"
     CDS             26233..26997
                     /gene="lenA"
                     /locus_tag="PLF66_11175"
                     /inference="COORDINATES: protein motif:HMM:TIGR04457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein LenA"
                     /protein_id="HMZ64649.1"
                     /translation="MRKRQILFIFLLIFIVTCKQKTKEPEAQILGTRYSKIDQHITSV
                     AGSTKKEHRVTLVYALEEVNVLEKISQDNKDFFKLSTVTGKEGYAQASNFSEAVLFVV
                     KEGLSAFRKPTLTAGTKGRFSIGAICFVKEIQGEWSNVDCITANVKDGVIEDWFDVWI
                     QSADERLSKDPMLGQTALLIRDAYKTINKANKLTGTDREKAMIDAKDKLAKASEKEDI
                     LQPVVTSIMVKYGMLDEPPATPTKPPEETQSPNNTQ"
     gene            27019..28731
                     /gene="ilvB"
                     /locus_tag="PLF66_11180"
     CDS             27019..28731
                     /gene="ilvB"
                     /locus_tag="PLF66_11180"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004427180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HMZ64650.1"
                     /translation="MSGTEKKITGARLLIELMEEAGIEVCFGYPGGAILPFYDELYKS
                     KIKHYLVRHEQGAIHMAEGYARATGKIGVCIATSGPGATNLVTGLADAKMDSIPILAI
                     TGQVATSAIGTDAFQEADIFGITIPITKYNALMRSADDIARHFEEAYKIALGGRPGPV
                     LLDFPKDVQTQLTSVRKAPKLKIAKHHYTNPEIKGDVKAFAEAINNAKRPLLYVGGGA
                     INANAAIEIKELALAGNIPVTTTLMGIGSFPGNHPNSLGMLGMHGTAYANKAVLDCDY
                     ILNLGARFDDRVAKIGEFAENAVRAHIDIDTAEFNKRIDVDYVLHGDLKDAIKAIIPH
                     IKKVDRTDWLNHIQKLKENHPLEYDNTADNQIKPQDFLDRLYKKTKGQCIVTTDVGQH
                     QMWAAQYYLFEEPNNWLTSGGLGTMGYGLPAAIGAKIGRPDKIVICVSGDGSIQMNIQ
                     ELATITMYKVGVKILIFNNNFLGMVRQWQELFYEERFSHSEWNYNPNFVKVADGYDIP
                     AMSISKKEDIDKALEFFLKDDKSALLEVMVPAEEKVFPMIPAGKSQKEMLEFKDLAKL
                     KAAK"
     gene            28728..29210
                     /gene="ilvN"
                     /locus_tag="PLF66_11185"
     CDS             28728..29210
                     /gene="ilvN"
                     /locus_tag="PLF66_11185"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016560825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HMZ64651.1"
                     /translation="MKHTLNILVNNHPGVMSHVSGLFTRRSYNIDSIAVGVTENPEVS
                     SMTIVLRGDDSIVDQVKKQLLKLPDVLKVTDLFYMEAITRELVLVKVMADDNSRTEII
                     SLCGVFGAKIVDVTDSSLMVEFSGNARQVTAFIAMMAKHGIIEMARTGQIALACQSGS
                     "
     gene            complement(29475..29807)
                     /locus_tag="PLF66_11190"
     CDS             complement(29475..29807)
                     /locus_tag="PLF66_11190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64652.1"
                     /translation="MNAFQFYGANLYSLDCIKTAKIYKDLFSLSILVASENHAELKTE
                     SGITVFIDRPSVHCFVTPGTVTFTVPSLDVDNLDLSPLVLESYFEKGRYASYLDEYKN
                     RIWIVEKP"
     gene            30024..30401
                     /locus_tag="PLF66_11195"
     CDS             30024..30401
                     /locus_tag="PLF66_11195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004783722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HMZ64653.1"
                     /translation="MKKLTRHRKIILENMQSRKDHPTAKMVYDSIRSNVKSLSFATVY
                     NSLEYLYNNGYIKKLDSFSDSAHYDAVLDKHSHLICRHCGKIIDFPNIDITDKLSSLT
                     GIFEAEDVAVTVRGFCQTCLDKK"
     gene            30406..32217
                     /gene="mutL"
                     /locus_tag="PLF66_11200"
     CDS             30406..32217
                     /gene="mutL"
                     /locus_tag="PLF66_11200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HMZ64654.1"
                     /translation="MGIIHELDVNLINKIAAGEVIESAHSVIKELLENSIDAKSNQID
                     IQTESGGLSQILVSDNGTGIAEEDLPLAVKRHATSKIKNLDDLETVLTYGFRGEALSS
                     IASVSKLKLTTGTSQNSPAIEILCDKGEILNKKNTTGFKGTKIEVEDLFYNTPVRRKF
                     LKSEKAEDKKIKDRIAITAIANPNIGFRYIQNQKEIFNLKPESTRERIVSVFGDNLEN
                     HLLEVNSEKKGIKGYGFISDPEFYKSNRSGQFLFVNGRPIEMRYSSYLLKKAYDELIP
                     TQAHPWCFIFFEIDPKHIDVNVHPTKKEIRFLDEEGFNSFFLQMIHAELNSKTPVGIL
                     EMRKRFFNEGKRAEAIARANSLLSPTFFSTNTNSQSRDTQEILHDSLYEEVKEQVPFS
                     VETVGAGTNLHELTQTPERKREFIPKKHFGIIFETFILAEAEDGIYIIDQHTAHERIR
                     YEEVLNQLKEKNLTQQLLTPIRIDLAKSDAEEILERKDEYAKVGITLDDLGGGTILVR
                     EIPVFLDPGKEKETILDFLNRTKGEKVLEKELYDLMAKCVACRSAIKKGDHMSDHILG
                     EILNRLSYCENPSRCPHGRPTLIKLTRDDLEKMFHRK"
     gene            32218..32814
                     /locus_tag="PLF66_11205"
     CDS             32218..32814
                     /locus_tag="PLF66_11205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012388284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HMZ64655.1"
                     /translation="MDKDLRKLIIQSVIGIVLIIALVILLAHIVKGPITQFSKLFVDY
                     LGVWGVGLGILVSDSLPAFMVPDAFLVFAVAGKLGDFEVILFCSIGSLIGGSNSYAIG
                     RYLIPRFESGRNLIKKHEAKLLPYIEKYGMLAVVIAATTPLPYSWMSILVGSFKMSFS
                     KFLLCSLTRIPRFAVYYYAIKFGWVEDIVFFLSQIYFT"
     gene            33244..34071
                     /locus_tag="PLF66_11210"
     CDS             33244..34071
                     /locus_tag="PLF66_11210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016751599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HMZ64656.1"
                     /translation="MKTKFEFFEIEIEDHIAIVYLNKPEKRNAMDWSFWRDLPEVVEE
                     INKDETIRAFIIAARGKSFSTGLDVLSFTSQVEEFLVPNNADTRKRFYNHIQVMQSGI
                     NAVYNSNKPSIAVVQKHCIGGALDLISACDIRYCTTDASFSLREVKVAIVADMGSINR
                     LPAIIGQGYTRELALTGRDVSAEEASKMNLVNGVYPTLEEAMKKAKDVAKDIASSSTL
                     VVEGVKEVMRFSEGKPMEAGLNYVCVWNSSFLASSDFDAARKAFATKTKPVYNENKK"
     gene            34068..34910
                     /locus_tag="PLF66_11215"
     CDS             34068..34910
                     /locus_tag="PLF66_11215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017861832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64657.1"
                     /translation="MSFISILRQALYEESMKHYSRAFNSYHKAIGLTKNPRTIARLTA
                     RKGWCQQYVGNQDQAQQTFSDLAKSAEVPEAYLLTSIYYLKTDRMKSAKNMLNKGIEK
                     FPEYLELYLTLASLLKDTERANESIEVLKKALSQEKLTRGKGGIERKDIWAELGNLYY
                     ERGSYNSCIVCLKRSLKMGTDKNFLHYALISKSYLKLNDPLNALKYIDLHLSYFEDLD
                     PEDFITKARAHARLGDNHLAGANLLQAYDSDGFLRLKTEEMADFSALVQSGFFTTLEN
                     LEIY"
     gene            35223..36341
                     /locus_tag="PLF66_11220"
     CDS             35223..36341
                     /locus_tag="PLF66_11220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64658.1"
                     /translation="MKKLISLFVSLILFYCASPDQKGDGFIYVQNITGINLDDAELNA
                     KRAIVAKGLGELVEGRTDVNQGQLMESVVNTSVEGFVLDYQQIGKDRKKGALVEIDAK
                     GKVDRGTAQSIIDKLLSQAGNPNFLMLVDEEIDKKKSPPTAIATENSIVKNFSEFNFL
                     DREQFRRVLAKEGGKTIGVYGDPSAEAKATEVAAQQGAEILFIGQTSVKNNGPIMDSG
                     MMSMQATVRFKIVDVGSAKIIAADNAGGAAPHVDVDRGAQEAIDKALVQSKPKILDQI
                     RTKWKRGMTIRLVIDGMNYDDFVDKGITGTIRSMKGVNAVNEKGSSNSNNSIVLEVQA
                     DFNGIVLYRRMRERREDLGIKFEQKEVKPGNVQIKVNK"
     gene            36486..37544
                     /locus_tag="PLF66_11225"
     CDS             36486..37544
                     /locus_tag="PLF66_11225"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HMZ64659.1"
                     /translation="MLKTIFPISFSNLPTPIKIIGTYFLITPIVSYIATAYKLDLPLS
                     NVKMILASFDKLNIILNLMGFLVGIGILGVKKWGYFVFLTFNSALIIHGIYLLVRFNG
                     IETEVMSQERLILNLIITILPFFLILYFLNSEISTPYLTLVPRGFRKKWRTEIPIKGF
                     LLDSLGNKLNITTLDVSPTGCLAEVDGYIADEDDFALTFDLEKDWRVSASVVRIQNEN
                     VGLRFNYNGTNDPAKKELKNYLESKLHPRYDITIPCDWSVNGQNYTSDILNISEGGLF
                     LSTNESAKIGDNVSFSFKLLGFRFSGTGSISWINSSAKYNKPVGIGISFNGLKKNILY
                     NFIISGFRLFAGFQSRER"
     gene            37708..38034
                     /locus_tag="PLF66_11230"
     CDS             37708..38034
                     /locus_tag="PLF66_11230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64660.1"
                     /translation="MESAILSNPKFIYKEGKPVEVILDIETYTKILDILERKDKKKKQ
                     IIVEMNGATLIRNSEAKTGKDIAKFAGIWKERNEIKNSLDYARALRKKAHQRPSIQEA
                     IKSLNK"
     gene            38238..38411
                     /locus_tag="PLF66_11235"
     CDS             38238..38411
                     /locus_tag="PLF66_11235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010415246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system, toxin component"
                     /protein_id="HMZ64661.1"
                     /translation="MNQALETYSAVKLKSGIDFIDCLIGHTAISLKIPLYTFNEKHFK
                     FIPNLKIVKPYKK"
     gene            39308..40294
                     /locus_tag="PLF66_11240"
     CDS             39308..40294
                     /locus_tag="PLF66_11240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64662.1"
                     /translation="MSVTNLLITIHGINPCLEPKDLTEDFKKLFDSMVAQRPMLNNLF
                     PSDHRIYIQWGHPPNENSKENIFTIGRAQSKILKLLDYDKSERTNNDIKLNNFFPNNL
                     NVSYILSKLLRENFVLTGLGDAAYYCSEEGENEVRSYIYEIILRKLEELKKKNSRIRI
                     HFIGHSLGVAICHDILFGLSYNGQKYEPGFLKNALGKEDLLKLFKFWRQEMLSHAKLE
                     AGSFISLASQLPLFIQRKHDILKKFINEENYKLDLTDIGISGTQLKWLLFYDLNDLLG
                     FRTRPLYHDPNNIVKEIEVDCGVLPDSAHLNYFKNPTVIWESAKLLEMNGRD"
     gene            40294..40890
                     /locus_tag="PLF66_11245"
     CDS             40294..40890
                     /locus_tag="PLF66_11245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64663.1"
                     /translation="MINFNLDAFTKYVDLLKFIVEFIALLIGSGFGFYRFILKKEFHP
                     KIQFSVELNVIDRTSKGWLVEVIAFIENKGSVRHLIDTNDFTFTIRYLTKKSNFSNDI
                     TIQKEKFYTLNFDNKVEDSTNKGYFQMLPNFFGETFIDPSVKQKYSCPLVILNDATHV
                     LVISKFRYKDLSMDFHTAQGFYKLETNAKIEQKQNQGD"
     gene            40893..41276
                     /locus_tag="PLF66_11250"
     CDS             40893..41276
                     /locus_tag="PLF66_11250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64664.1"
                     /translation="MRKSVSILLVLVALAMIGCASGDSGNSSEPVRENSQKSDGVESE
                     KQRGIDWIKENYIDKFEQAPVLKMFDKHTSAGKNYILVFGGKLSKTKKATEFYWRNNT
                     FTTRELSEPEMKRMDLCNGCTKIFE"
BASE COUNT        14418 a         6788 c         7008 g        13219 t
ORIGIN      
        1 ttcttgtaat ccaatttctc cagatcttta aaatgctctc ttagtagaat agcaagatta
       61 gtgtcctctg gtcttaaaat ttgagcttga attaaaagtg tttcttgtat actacttgca
      121 ccaattccaa caggatctaa ttggtgaata ttttgaatca cttttttcac taatttaaag
      181 tttagattca tttccttagc tatctcatct aacggtgttg tgataaaccc atgctcatct
      241 aacatagaaa ttagaatttc accaatctct aactcttcat cggttacatc taaaagtctt
      301 aattgtgaaa gcagatggtc gcttagtttt tctttgatgg gagatgcttc aatcatactt
      361 tgatttttat ctctcgcttc gttgtcataa cgatagggac cttcaatgct gtaaatatct
      421 gtgagatttc cttctgtgct tttagtaagt tctctttttt ccttctctct gatttctgtt
      481 acagaataga tctcaggagt tttcggtttt tcaggattaa aaacttcttc taaaagtgga
      541 ttgtcgatta gttctgcttg gattttatct gcaagctcta aagtcgataa aggcaataat
      601 tctatagact gccgtaaatc agcagtcatt actagttttt gggtttgctt ttgaactaaa
      661 tgttggccta acattagagt gtaaaatcct cccctagata aattctcctt gtttctggat
      721 cgtttactag ttcttgggta acaccggaaa ttaaaatctt tccactatac ataatatatg
      781 ctctgtctgt aatttttagg gtttctctta cattatgatc tgtgattaat attccaagtc
      841 ctctttctcg taagctttta ataacagttt gaatatcctt tactgcaata ggatctactc
      901 ctgcaaatgg ttcatcgagt aaaataaaat caggcttggt agcaagtgct ctcgctatct
      961 cacaacgtct tctttctcca ccggataacg taaatccttt ttgatttgct actcgcatta
     1021 tctgtaattc taataataat tcatctcttc tttcttgaat ttcttttctt gaaaggttta
     1081 atgtctgcaa aacagcttcc acattttctg caaccgttag ttttctaaaa atagaagctt
     1141 cttgtgctaa atatccaatt ccaagtctag ctctttgata cattggagct tgtgttacat
     1201 catgatcatc aataaaaaca tgtcctgaat caggtcttac aaaacccact gacatataaa
     1261 aggatgtagt tttaccagca ccatttggtc ctaataaacc aacaatttcc cctttactaa
     1321 ttttaaaact cacaccatct acaacctttc tcttattgta gatttttact agattttcca
     1381 ttctgaaagt tttttggctc attttttatt tacattcagt ccgtctgata aaataattcg
     1441 atccgaattg gggtaaataa tgattcgccc acaatatact gtcttttctt cttttttaag
     1501 actcggattt ccctctacat acattttttc gctctcttca aaataagtag cgtattctcc
     1561 atgaattgtt gaatcactgg aaaccaaatt tacatctcct ctacaaacaa tctccttctt
     1621 atctccaaat ctttctactt caatagcggt catactagaa tttacatttt cattttcttt
     1681 atctaaaaac tcgatcattg cattgtcggt catatgggaa taatcttttt ccttactatg
     1741 ttccaaagta tctcccgata gacgcacgtg attttcttta tcaagtagag taacgccctc
     1801 ttttgcttct atatttccat tttcttttcc aaatgctttt atataagatc cggtgaactc
     1861 aaaatcatct cttgtcattt ttgcatttcc aaacatcccg ataaagcgag tctcctcatc
     1921 tccagattga ttgattagtt tatcgcctgt aaaaatactg atttttttta ttttttcagt
     1981 ttcttccggc ttatctgtat tatccgcttc cttagaatct tttctgattg tctttttttt
     2041 gggattttca aaactcaatt taagtagaat tgtatcattt tctagaacag tgttcttagt
     2101 cgaattggag taagttactt tctctcctgt taaaaatgtt tcttttccaa agataaacgg
     2161 gtaaccctcc atcgtaagag attcttcttt ctcttcaaag atagcagcgt ccgaatagat
     2221 agtataatca ctgttttcaa ctatcgcacc tccctcaaac acagtcttgt tatctgataa
     2281 ataacgtttt aaatagggag ctgtaatttt ggttactttt ttttctttat ctacaaatat
     2341 aagagtgggt cgaccttcta aaataacagt ttcttgtgct ttgtcataaa ttcctttccc
     2401 ggcagttaag gtaatcccat tctctggatc ttctactttt gtttgtcctt ttaaatgtcc
     2461 tctataagca tcttccccta ctatttcaat gctattggca gaaagtttta cttttttatg
     2521 taaaatccac gctccaccgg tcaaagaata aaccgtcata tctatacctt caacattttt
     2581 tgtttcttgc gatagtgttg cccctcccca agttgccgga attttggatt ggtttttttt
     2641 agaagacttt gcagtttcta tgtatgggaa attaccatcc gaaagattta aaggagaaat
     2701 aagtaaagct ggtcttgtat ttgcctgaat agatagcaat ccactgaaag taaaaataat
     2761 ccaaataaat ggtaagttca gtttgtttcc tttctgcgtt ttcatgggtc ttgatgcaag
     2821 attgaaagac gttcaaaatc tttcaactaa tgattcaagt tctatgccac tttgtaaaaa
     2881 tttctgactc ataaaaaaat ctttttttga aaaagttttt aactaaatcc tctttacatg
     2941 tatagataga tttccaagac ggctttgaat ctctttttaa tccatttctc accatttttt
     3001 gtatttgcga ataaatcgtt tgactttaaa tctgatagtt agaaatattt ttagtaatat
     3061 tatgaattga gttctttgga atctgtaggg aatatatgaa tttaagactc attgaaaact
     3121 atgataggct atgttaaaga aaaaaattaa tataccaaac ctttcctttg aagaaatgtt
     3181 tttcaatagt gatgcaattc atttactcat tgatccagag actagcgaaa ttatttgtgc
     3241 aaatcctgct gcagaacgtt tttacggttg gtcaaaagcc gagctcgttg gtaaaaaaat
     3301 tcaagatatt aatacactct cacccgaaga aattaaaaat gccttggatc aagttaagaa
     3361 taacagacaa ggtttctttg aatataaaca tcgcacctct aattcaattc gagatgtttg
     3421 tgtttttagt aatgtcatcg accataatgg acaaatcttg ctgcactcat tagtgtatga
     3481 tattacagaa caaaaaagaa aagaagaaac aatccaaaaa caagaaacat ttttacgtca
     3541 agtggaaaca ttggcaaaaa ttggaagatg ggaagtggat atactaaatc aaaaagtatt
     3601 ctggtctaaa gggatgtttg atatttatga actagaagag aaaaaggaat cattaaattt
     3661 tgattatggc ttaaatcttg ttcatccaga tgatagagat attttagtaa atgccttcca
     3721 tcattctttg agagaaaaaa ttccaattac tatggaacat agaattcttc taaaaaatgg
     3781 gaagattaaa actgtaaatg agacttttaa aacagatttt gatttgaaag gaaatccaat
     3841 tagaacgata ggcgtattac aagatataac agaccggatt aaaacagaag aggcacttcg
     3901 tctttccgaa actacaatga aagctatttt tgataatagc tacgaatcct ttatttttat
     3961 ttctgaacaa aagcaaattc tattttttaa taagcaagca caggatagaa ctaaaagaac
     4021 atttgatgta acgcttcagg taggcgattc tatttacaaa attgttcagc cagattccct
     4081 aaatcttttt aattctaact ttgaaagagc ttggaatgga gaaagagtag tttacgataa
     4141 acattttact atcaataatt taattgtttg gtatgagttt caatttatac cagttaaaaa
     4201 tgaaaaaaga aatacaattg gagttgtgtt aacagcaaga gatattactg aaaagaaaaa
     4261 agcagagtta gacctaaaaa ttagcgaagc aagatttcat gctatttttg atcaggcacc
     4321 gttaggtata gcgcttattg aatccaaatc gggcaagttt atccaaataa atccaaaatc
     4381 aacagaaatt ataggttata ctgaagatga acttctaaag atggattacc aacgtatttc
     4441 ctatcctgat gatttaaaag aggatttgga aaatatggat aaattaatcc aaggatcaat
     4501 tcaatggttt acaatggaaa aaaggctaat ccgaaaggat aatagggtta tatggactag
     4561 tcttacttgt gttcctttaa aactagaaga agataatttt acttaccata tagtaattat
     4621 aacagacatc acagaaagaa aaatggcgaa tcttcaacta gaagatttta ctgagagtct
     4681 taaaaaattg aatgcaacta aggataaatt ttttagtatc attgctcatg atttaagaaa
     4741 tccatttatt ggaattttaa atttgattaa agttatatta ctaaatatag ataaaacgga
     4801 taaatttcac gaaaataaaa aatacattga gttaattcaa gactcttctc gctcagccta
     4861 tgacctactt gagaatcttt tacattggtc tagagcacag acaaacgata tagatttatt
     4921 tcctgaaact atttatttaa aaaaactagt tttggaagta gttaatttat tcaaaggaaa
     4981 tgccgctgca aaaaatatca atattgagat tgaaatatcg gacaacgttt tcttcttcgg
     5041 agatgaatat ttaacgagta ctattattag aaacatttta tccaatgcat taaaatatac
     5101 atacagaaat ggaagaatca atattttctg tagagatttt actgatagaa tagaaattct
     5161 aatacaagat aatggaatcg gtatttcgga agaaaattta aatcgtcttt ttaaaataga
     5221 tgaaaagttt acagaatctg gaacagaaca agaacaaggt tctggtctag gtctcatttt
     5281 acgtaaggaa tttattgata aacaatctgg attgatagaa gtaaacagta agttaggtga
     5341 aggtagtact tttgtgatta cattgccaaa gcaaagtgcc tttgaccttt aaaaatacga
     5401 tagtcttaaa ggaatctcag tagcaaaaaa ttcgcttgcc attttatgtc taggttttaa
     5461 attcctacta ttctaaagat aaaaccagaa tgaggattag agattttata attagtaaat
     5521 aataaaatta tatctcacaa taaaatgcaa cactattcat tcacagatgc caacttaccg
     5581 ataaaccaaa tcattgatac tcttcccatt ggatttgttt atcaaaatgc agaaggtgag
     5641 attctttatg caaatctagc agcacagcga attcttggtt taagcttttc gcaaatcaac
     5701 ggtagaaatt caaatcaccc agattggatg tctattcatg aagatggaag ttttttctcg
     5761 ggtgatgaac atccaggacc tatggctctt cgaacaggta attcaatttc aaatgtaacg
     5821 atgggaattt tcaatccaat aaaacaacaa tacatctgga ttcaaattga cgcagttcca
     5881 gtttttattg aaaacagcca gaaacctatc ggtgtctatg cggttttcga agatataaca
     5941 gaatataaga aaaataaaga tgttgaaaag gatttatttg taaaactcag aaaatcaaat
     6001 ttagaagtaa gccaaagaca gcaagcaata gatcaacatg cgatagttgc tataactgac
     6061 tataatggaa ctattatata tgcaaattca aaattttgta cactcagtaa gtattcacga
     6121 gaagaactaa taggacaaaa tcatagaatc ctaaactcag gataccattc aaagtatttc
     6181 ttccataata tgtattccac tataaaaaaa ggagagacat ggcatggaga aattagaaat
     6241 caagccaagg atggaagtta ctactgggta gcaaccacaa tcgctccttt gcgtaattta
     6301 gatggcgaaa ttgaacattt tattgccatc cgtacagata tcacaaaaag agtgcaggca
     6361 gaagaagctc ttcgtatttc cgaggcaaga actagagcca ttttagatac aagtccagac
     6421 tcgataatct tcattgattt agattatcga attcaatttt ttaatgatac agcaaatagt
     6481 agatcaaaga tatttttaaa taccgaatta acgattggga aatctatgtt acatatttta
     6541 cctgaaagta gaattgaaaa ttttactctt tccttccatt ctgctttaaa aggagaaata
     6601 atcactcaca ttcgaaattt ttccacaaat tcaggaactg tatggtttga agttttatat
     6661 actcctataa aaaataaatc ttctgaagtg attggagtca tgtttactgc aagggatata
     6721 actgcaaaaa gagaagcaga aactgaaaga gaacgttatc tggaagatct tgagaatcta
     6781 aatcatacaa aggataaatt ctttaatata attgctcatg atctaaagaa tccgtttgct
     6841 ggaatcttag gaatttccga aatgcttgag agcaagctaa aagaatcaga gagccaatat
     6901 acgaaagaag atcttttaaa gatggcaaat ttaattcaaa gttccgctag atctgcttca
     6961 aacctattag ataatttact ccactgggcg cggtctcaaa ctggtgaatt acaatgtgat
     7021 ttaatcataa catctataaa ctctataatt gaagcgacaa ttcctcttat ctctggaatt
     7081 gcttttaata aacaaataga aattgaaaaa aacttagatg ctgaaaaatt cgttttagct
     7141 gatccttctt taacgaatac tatacttaga aatttattta ctaattcgat caaatttaca
     7201 aatccaggcg gtaaaatcat agtaagaact aaattcaata atgactacgc agaaatttcc
     7261 attgaagata ccggcattgg aataaaagaa gagcaccttt ccaaattatt tagaatcgat
     7321 tctaaaataa caaaattagg aacaaacggt gaaaaaggaa ccggtcttgg tttaatacta
     7381 tgtaaggaat ttacagaaaa gcagaatgga aaaatctggg tagaaagcga atttaataaa
     7441 ggatctaaat ttacttttat tttaccaatt gcattataaa aaatggaatt aaatcaacaa
     7501 atcatgaatt caagttcgtt tgcacaagcg gctctggatg ctgtgtctgc taacgtatgt
     7561 gttttggata aaaatggata tattattgca gtcaaccaag gctggagaga atttttcgag
     7621 gaaaatagca acagttcaga aaataagagt attggattaa actatttaga catttgttcg
     7681 acctcatcgg gagcaagatc agaagaagct cttcctatgt ttaatggaat catgagtgta
     7741 ataaaagatg aagcaaaaga atttacacta gtatatcctt gtcatagtcc tacagaacaa
     7801 agatggttta gggctagagt aacaaaattt aatgacggta gtggaaatat tgttatcgca
     7861 catgaattaa ttactgagag aaagctcgcc atggatgcac tgagactttc tgaagctaga
     7921 atgcgagcaa tcctaaacaa tgtaaatgaa tcttttttat ttatagatct aaactataaa
     7981 attcaattct ttaactcact agctctaaaa agaatctatc atatgtttaa tattacaatc
     8041 caagaaggcg aaaacttttt agataaatta attgaaactg atagatacat gatgtcgacc
     8101 tatttcaatt tatgtaaaaa gggtgaggct gtagaaataa agaaaattta taaagtcaac
     8161 tcttatccaa gatggtatga cattaaattt attcctgtta aagaagagga tggagaaatt
     8221 attggaatac tttttacaac tagagacatc cacagacaaa agaaaatcga acatgaatta
     8281 gttcgaaaat ctgaaaattt aaaagagctc aatgctacta aggataaatt ttttaaaatt
     8341 ttggcacatg acttgagaaa tccatttttt ggaatttcag gtgttacaga aattttaaaa
     8401 gaaagactct cgaatattcc taattatgat acagctgaat tacaaaaaat ggtaaatttg
     8461 attcaagcct ccaccggttc agcctctgcc cttttagaaa atcttatgga atgggcaaaa
     8521 gcacaaactg gctctctcca aattgacttg gttgaattta atataaacaa agaaatcgaa
     8581 aaggtcattg acttaataaa aggtagtgct tttcataaaa acattacaat agaatttaaa
     8641 ttagagaacg ttaattctgt attagcagat tcctatttaa ccaatacgat ccttagaaat
     8701 ttattatcaa attcaattaa attcacacca attggtggaa acataaaaat tgtagtaaaa
     8761 caaactggta aaaatgtaga aatagccatt acagatacgg gtataggtat ccctgaagaa
     8821 agtataaaaa aactgtttcg aattgattct aaatttacaa tgcttggaac agaaaaagaa
     8881 aaaggaactg gtcttggtct aattatttgt aaggaatttg ctgaaagaca aaatggattt
     8941 ataagagtac aaagtgctgt aggtgaaggc tctacattta ctttgtgcct tccacatatt
     9001 taagaaaaga tataattctg cacttggaaa gtgaactaaa aatagttcac tttccaaggt
     9061 ctattttcgt ataaactact taaacgtctg ttaattcact aacgcttatg tttaatcttt
     9121 ttgataaacg aaatagtgtc ttgagattcg gatttgattt tccagtctca atgttctgca
     9181 ttgttcggtg aggaacacca tcgtctccct catccatatc ttcttgagta aaacctcttg
     9241 cgagtctttc ctcttttatt ttttttccta atttgtttct aaatgtttca aagttcacac
     9301 ttcgaaagta aaaataagat taaaaaaagc aagagaaaaa tttaatgagg catttattta
     9361 tatttaattt atgaaaaata aaataacaaa atgcaatcat tttataaaag cagtaggaaa
     9421 tgctataggc aaaaaatttt atactccaaa tgtggataat acagattatc ctctttttga
     9481 ttaaaaaaac tcaatatatg aatttgaatt tcaatctaaa taattttaac tatttttaat
     9541 ttaaaaaaaa ccatatttag tatattttac acacaatata atgtatatat gtgaaaatat
     9601 gcaagaaaat caatgctttt aggagatttt taggaactaa cgatcataaa aaggttttgt
     9661 tttaaatttc ttttaatatt tgaattagtt taacagcttt ctggtatgga ttctctgtta
     9721 tcaatatttc ttgttttctc ttataatcac aattaatcac tgaagtaata aattcaacag
     9781 gaaagggatg tgatgttagt ttttgaattt catttatata aacattttca acatttagct
     9841 tcatgaggta actccgtgtt agccttatta gttcatttgt taattcttta aattcttctg
     9901 tattaatatg tgagtaatca ttatcaattt tatcaacttc agcaattata aatggttccg
     9961 ttgaattgaa tttttttaaa atagcaattc ccattccttc aagtagtatg tttgcccttc
    10021 catcttttag tggttcatgt cgaacaattt taccccaacc aaaagtagtt tcaatttcag
    10081 aatcactttt tagggctgta actgccatct cataatctga ttcaatacaa taatcaagca
    10141 tcaatctata tcttggttca aagatgtgca aaggtaaaaa tgcccctgga aaaaaaacca
    10201 tattgggaag tggaaaaaca gctattgttg aaatcatatc tctattaaat ccttaatttt
    10261 cccttttgtc aagattgtaa gaagaggata tggcctgaaa attgtattta cagtaataat
    10321 agccatgcat tcttagaatg aggggacaat tatggctaaa aaaatcgcaa agaaaaaaga
    10381 aacggttaag attcaaaaac ctgagaaaaa gacagacgag tttaggaaag aagtagatcg
    10441 cctcttccaa ctccaacaag caaatcgctg gaatattgca aatacgactg ctaaccaaag
    10501 aattgaaaaa ttaaacaagc taagatcggt cattgaaaag tattctcaag aaattagaga
    10561 tgccttgcac agtgatttta gaaagagtcc acaagaagta gatctaacag aaattatgcc
    10621 agttatttcc gagataaaag atgccgttct gcatctaaag tcttggatgc gtcccttgcg
    10681 tgtaggaact ccccttagtt tatttccaag ttccagccaa attatctatg aaccaaaagg
    10741 cttaatcctc attataagtc cctggaacta tccatttcat ttgataggaa ctcctctcgt
    10801 ggctgcaata gcggctggaa attgcgtaat tattaaacca tccaataata cgaaacacac
    10861 tgctgcaata accaaaaaaa ttgtttcaga gatattcaat gaaaatgaag tagccgttgc
    10921 ctcttcggat agagacactg cggcagccct tttagaaaaa cctttcgacc atatcttttt
    10981 tacaggaagc acatctgtcg gaactactat tatggaagca gcagccaaga atcttacttc
    11041 ggttactctc gagttaggtg gaaaatcccc tgtaattgta gatgagtccg ttgatatgga
    11101 cgatacaact gcaaaagtaa tctggggcaa agttctaaac ggaggccaaa cctgtgtagg
    11161 tgttgattac gttttaattc atcaatctaa atataatgaa tttatagagt catgcaaaac
    11221 tcaggtaaaa aagttttacg gtcaaacccc tgaagattgg caaaaaacac ctgacctatg
    11281 tagaattatc aatgataaaa actttgaacg attagaagac attgttaaaa atagtattaa
    11341 acaaggagcc aaactagaat taggtggtga ttttaaaaaa gaagaaagat atatttctcc
    11401 aactatttta tctgaggtta cacttgatat gccagtcatg caagaagaaa tatttggtcc
    11461 aatcttacca attattccat ttaatacgtt agacgaagca atcgcgatta ttagaagtaa
    11521 acacaaacct ttatcacttt acatcttcag tgaaaaagat gctaccattg aaaaaattct
    11581 aaagaatacc tcctctggtg gagttgtgat aaatggagtt gtagctcatc ttggtaaccc
    11641 tgaacttcca tttggaggag tgaaccatag tggaattgga aattatcatg gacattttgg
    11701 gttcaaggct ttctctcatg aaagagctgt tttaaaagta tctaagttaa cacttttaaa
    11761 aatgcttttt cctccttata caaaaactac taaaactttc gtagatttta tgacaaacta
    11821 tctctaattg agaaagtttc tgatttttca ctttattatt ccatacagtt tggaaactag
    11881 gctgtatgga aatagaaaga cttaagtttt taacagaaga agaaactcgt tctatagcga
    11941 aagagtttgg aactccagta tttgtttatt ctcaaaagga aatagaaagt cgttgtgata
    12001 ttgcacttgc ctttccaaac gaatatggtt tgactgtgcg ttatgcgatg aaggcaaatc
    12061 caaacaatgc cattcttaaa ataatgaaga aaaaggggat acatattgat gcgagttcag
    12121 gatttgaagt ggaacgagca ttacttgctg gtttcaaacc ttctgagatt atgctaacct
    12181 ctcaagaact tccaagtaat ttaaaagaaa tagtagaaag aggaactatg tttaatgcat
    12241 gttcactcca tcaattagaa agttttggaa aactttttcc aaatcaggaa gtttctgttc
    12301 gaatcaatcc ggggctcgga agtggagcaa ctcaaaaaac agatgttggt ggaactacat
    12361 ccgcatttgg aatctggcat gaatacatag aagatgttaa aaaaattaaa gagaaatatg
    12421 gtttaaaaat tacaaaagta catactcata taggttcagg aagtgatcca gaagtatgga
    12481 aagcagtttc taaatacact ctactctatg cagaaatttt tacagaagcg acaaccgtaa
    12541 acttaggtgg tggttttaag gttggaagaa tgctagagga gaaaacaacc gacctacaaa
    12601 aaatcggtca gcctgtaaaa gaattattta tagaattcaa tcaaaaacat ggtcgtaaac
    12661 taaaactaga aattgaacca ggcacacata tgattgcgct ttgtggttcc attattacca
    12721 aagtcatgga caaagtagat actggaaaaa atggattcga atttataaaa ttagataccg
    12781 gaatggattc gaatacacgc ccttctctct atggttcccg acatcctctg attacagtaa
    12841 cggctaatgg tagtttaccg caaaaaacaa aaaactatgt ggtggttggt cattgttgtg
    12901 aaagtggaga tttatttacc caaaaagaag gtggtagtcc ggaaactaga gagatgatgg
    12961 aagcaaacat tggtgatcta gttgtaatgg aagggactgg tgcttattgc tctggaatgt
    13021 ctacaaagaa ttataattct tatcctgaaa ctccagaggt tttaattaaa tcagatggtc
    13081 aaaaagtttt aattcgtaag aagcaagaat taaaacaaat ctttgaaaat gaaatcgaaa
    13141 tagcagtgta attacactgc ttttttcagt aattctttag catgatcgag agctcctttg
    13201 gtaatgtgtt ctcctgcgat catttttgct aattcctttg ctctctctga atcagaaatt
    13261 tttctagcaa ttgtaaacgt tcttccattc atgatatctt tttcaacctt gatatgacaa
    13321 tcgcttacag ctgcaatttg ctgcaaatga gtgattagaa gaacttggtt cttttcagag
    13381 atagatttta atttatcagc aacacgatag gcgacctccc ctcctatacc cgcatctatt
    13441 tcgtcaaata ccaaaagtct tggctcattg ttattcccta atattgtttt catagcaagc
    13501 ataacacgtg acatctcacc acctgagatt atttttcgta aaggtctagg cttctctcct
    13561 tgatttgcac tgaaataaaa atcaatttgg tctagtccat tctcattgag tatatatttc
    13621 tttcctccgt ctgttacttc tccatcgatg gcttgttccc atctcattac aatctgtagc
    13681 tttgcatctt tcatcccaag taattgaaac tccttttgca aatcagattc taattttaaa
    13741 atagagtccc ttctcagatg tgataatttt actgctagat ttgttagatt gtcccgcata
    13801 gaatccaatt ccttttgaag agagatcact tcatctgaac ttatctctaa actttctagc
    13861 tcgatctttg ctttttctaa aaaatcaaga attgcagaaa tgtcttttcc atactttcgc
    13921 tttaatttat taatttcatc taatcgattt tgcactatag acaatcgatc actagaaaaa
    13981 aatatttcat ctttttgatc gattacggta tgcgatatat cttttaattg ttcatagatt
    14041 tcataaagtt ggtttcgatt tgtagaaaaa tccttggtaa ccgtttcaat tttatctgct
    14101 gcatttaaaa ttttagaaaa tgattttaat atattttcct ctccttcatt tagtaaatca
    14161 gcaattaaat tgtaattctc aacaatcttt tctccgtgga tcagcatttg ttcgtcttgt
    14221 agtaattctt cttcttctcc aagttgtagt ttggcagatt gaatttcctt gatctgatat
    14281 tttaaaattt cgattctatg gtttttatcg gaactcattt tatcaatttc agaaattcta
    14341 ttttttaaag tttggtaggt tttaaatcgc tccgagactt cttttttaag agaggttaat
    14401 ccagcgtatc catctaaata ctctaattgc atagattttt cgagaagaaa tagttgatca
    14461 ttttgcctat gaatctcaga taataaggat ccaagttctt ttaaaaactg ggaaggtgcg
    14521 agagatcccc caatttgaat tctagacttt ccatcgctaa ataattcctt tttgagataa
    14581 atttctaaac cgtcatgctc tatacctttt tcattgagcc actctatcgc ctttggattc
    14641 ttagaaatat catatattgc ttccaattga tatttcttcg ctcctgagcg tatgttcata
    14701 gtattgcatt tgctgcccat aagactagaa attgcatcta aaagaagaga ttttccaact
    14761 cctgtttcac cggtaattgc aaccatctct ttatctaatt ctaattccaa tgattcgatt
    14821 aatgcgaagt ctttaatttt gataagcttt aacatactgt ctccttattt tactccgccc
    14881 gggcacgcca ttagcacccg ggttcggagc acgtgtagca aaatcctgat ttacaggtct
    14941 acataaacat ttatgtagta tataatacta tacggagttt agtaaacaag aatttagtat
    15001 tttttaattc atttaaaaag atttattttt ttttattttt tgaatgaatt atatctgaca
    15061 gacagatttc aaagcaaagt cggatatttt cttgaattct gaatttcaaa aattcaattc
    15121 attcacaaga cgaatcccag acctatctat ttcaactcct cctccgacct ggatcattta
    15181 cttctacttg gaatggttcg tatccgagtt tgaaaagaaa ctgccaaacc tagtagtttc
    15241 ttttcgttat ctctacggtg ggcattttta gagtaatcct cctttttaat tctaacctag
    15301 agaggttgca tctttcatac aatattaaac cggaaagaag gattttatag ccataaaaag
    15361 ggaatcgctt gctttttaga ttgtcttccg tttgaagatt atccatattt attaaacaga
    15421 tcacctattt tataagcttt tttataaatt agctcatttt ttaatcacat atctttatgc
    15481 attttttaaa atattttatt ttttaaacat cttttatgta aattgttata tttttgagcg
    15541 tctattgaat caattatggg tacagtaatt gcaacataat aggaaggcta gattacaatg
    15601 atagaattaa aatatggaac tagaaaggtt atatcattta ggggagcaaa aaaagtggtc
    15661 ggagggttgt ctgaccaaaa taaaattgcg gtcttgcttt atataagtaa ggaattttcg
    15721 aatgttggga gagaagagga cctatttgat actttaatta cactctgtaa ggaaattttt
    15781 gaatgcgata acactacttt acgcctttgg gatggcgaat tgcttgttcc gattaagtat
    15841 attaaagaaa caacgccccc aagaaggaat ttaacaccat cggaagggta ttccggacaa
    15901 acattcagca ataaaaaacc tctccttata cccgatttaa agaaaaaccc tcaattcttg
    15961 gatgaccagg aaacaacaca atgcgtaatt tgcgtaccaa ttacctataa ggatgacgtt
    16021 ctaggcacta tttcagtcga atctgatacc gcattctttt ataaagaaga tgatctggaa
    16081 attcttgagg ctttaggcgc tcaattagct cttgctttga ccggagttag actaattgag
    16141 ggtctaatga ccgcaagagc gagagaggca gcaattctca cccaattaga atgggatttg
    16201 aaaatgggtc ggaatgtgca aagccaaatc ttacaaaacc aactcccgcc ttggaatggg
    16261 atctatttcg gaacccacta cgaacctatg gtggaagtaa gtggggatta ttttgatgtt
    16321 gtaaaacagg gaaataccat gactgccata attgtagatg tctctggtca cggaattcct
    16381 gccgcacttg taacaatggc aattcacttc catttcaatc gttttgttac acaaggtcta
    16441 ggtcttgccg aaatcatgga acaaatgggt gaggctttaa aaccccaatt acctgaatct
    16501 acctacttta ctgcatttat cgtccgtctc tttcatgatt acacctacgc ctacatcaac
    16561 ggtggtcacc aaaaagtaat tcatttcaaa aacgatgcaa ctatagatga tttagattca
    16621 ctcggcgttc cgcttggaat cttagatgta aaaaaatcag actatgaaga gatgcatggg
    16681 aaattagaac caggtgacta tctacttatg tttaccgatg gatttacaga acaaaaaaat
    16741 tcagacgggg aagaatatgg acaaaccaga tttaaagaat cttttttacg agctcgagat
    16801 ttattaaaaa ctgaaaagca aaatgtcttt gtaaaagata tcgtaaaatc tatgttatcc
    16861 gattggaatc aatttaaagg aagtcaaaaa aatggtgacg atcttgcaat gcttttactg
    16921 caatgcaata ctacattgac tgacgctctt cctttagttc gtatggcaaa agtttctgcc
    16981 agagaaaaaa ataatgcaga tgcttattcg ctcgcactac aagcctatcg catcgatcct
    17041 tctatcaaag ataatttatt attcttagga aaaatgtatt ataatgatgg gaaatatgaa
    17101 gaaagcgtta agttcataga tgaatatatt aaaaccagtg gcgaagacac tgcaattatt
    17161 cattatctat acggaaaggc tttgtttaac tcaaatcgta ttgctgaatc aaagagagca
    17221 ttaaaaaaat ctctttcttg tgatcacacc tttgcaaaat caagtctctt attagccaaa
    17281 tgctatttaa aagaaaatgc aatcccaaaa gctatcaaga ctctgcaaag aggaatcaaa
    17341 agcactccaa gtaacgatgc attaaaagta tctctaaaaa aattagaagg aatcaatata
    17401 atccgaactg caaatactat tgaggttgag gaagacaatt caggagaaga acaatgataa
    17461 aaggtttact aattcttatt tttatctctc tatcaatatg ggcacaggaa acaccggtga
    17521 atccagatat gccgctctct cctgaggtta ttgaaataaa tacaataaaa gccgatttga
    17581 gtcaatttaa aggtgtaatg gaatccatcc aaaaagaatc caactcttta aaagctgaga
    17641 tcaatctact ttggatttgc attgctgctt tcttagtatt ttttatgcaa gctggttttg
    17701 cattggtaga agctggattt acaagggcaa aaaatactgt taacatatta atgaaaaatc
    17761 tatctgattt tacaattggt tctatttgct tctggctaat tggttttagc cttatgttcg
    17821 gacctcatct attagaaggg ataggaatcg gaaaaatttc ttttttagaa acaagcttat
    17881 tactagaaga tggaaaacct tctcccttca aatatggttt ctttttattc cagttagtat
    17941 ttgccgcaac agcaacgact atcgtatcag gagcaatggc agagagaact aaatttactg
    18001 cttatttgat atattccttt ctaatgacag cacttattta tcccatattt ggaagttttg
    18061 cttggtccaa tttatttgat acaaataatc caggtttttt agctaaaatg ggttttgttg
    18121 atttcgctgg ctcaaccgtt gtccattcta tcggtggttg ggcgggactt gcagggacga
    18181 tggtattaaa accacggcta gggagatatc aaccaggtgg agtgatcatt cctattcttg
    18241 ggcataatat gacgattgcg gctatcggtg tttttattct ctggcttggg tggtttggtt
    18301 ttaacccagg aagttcggga acaatccaag gtggttcgtt tgcgatcatt gcaatcacta
    18361 ccaatatggc agctgctgct ggagcaatat ccgcaatgac actcagttgg attctattta
    18421 caaaacctga tattggaata actctcaatg gaggactcgc aggattagta gcaattacag
    18481 caggatgtca cactgttgga attacatccg cagtcattat tggtatgatg agtgggctac
    18541 tcgttgttgg aggtgtttta tttttagata agttaggaat tgatgatcct gtcggtgcag
    18601 tgagtgttca cggattaaat ggcgcatggg gaacattagc agtaggtctt tttgcagatt
    18661 ctacatttgg gggtggtccg aatggtttat tttttggagg cggattctct cttctcttaa
    18721 cacaaatcat tggagtttta gtagcatttg tttggtcttt ttccctgagt tacttagtct
    18781 tttttacaat ggaaaaaaca ataggactta gagttactga ggatgaagaa atcattggac
    18841 tagatctatt agaacatgga aatgaagcgt atccagatta taaatcttaa taactcattt
    18901 taaatccata actaataaaa ttagaaccac aagcaggatc gggagggcag taaaaaccaa
    18961 agattcccga tctatgatga actctcataa aaattcttgg attacttggt agatcccaaa
    19021 ttctatagtc taattctacc attaaataat ttaaaatatt tctagactct atcgactgac
    19081 gttcgatctg accgttcttt aaattaaaac ctttactttg gttttcaaga attgggtttt
    19141 gactcccaat tgaaagacct tctccaaatg caaaactcat aggcaaacca aacaaattgg
    19201 aaacacgagc gatcataagt ccatctgcct cccaatgatt catgattcca aaatgcttac
    19261 ctactaacgc ttctgattca atctcaaaaa accttactct ataattcatt ggataattaa
    19321 tacctagaac accgatatat gattctctat aatcggtttt ttgtctaaac aggatgggaa
    19381 gtaaatctgt agaagtatac tttccaacat aaacaattag attgattttc tttttctctt
    19441 gaataggctc tgcgaaaata ggaaaaagaa agaggatcag aatgaaagtt atttttttag
    19501 tcattgattc ttacctatga aacgaattgt ttctataaaa ttgatttcta ccttattaaa
    19561 actgtcttgg agataactaa gataatccgc acagtattcc tctttggatg cttctaatat
    19621 ttttctagtt ctactcacaa cttctgtgag acaatatttt tgtaaatggt ctgggtttcg
    19681 ataagtattt agtattcctc caaaactagt tgcagcaagc cccgcataaa aaggcaatat
    19741 tgcccatcta ccgattcttc cttccgattt aattttcaac ggttcacttt cttggtttaa
    19801 attttttaca ttaaagtgaa ctgttgcaga atttgataaa aatactccaa gggttaatgt
    19861 agcaatcgac ttacccacat ttataaaata atcggacgaa gaatcgccaa tctttgcact
    19921 gacagaaata aggattgaat ctttatctaa aagacttggt tcggatatac actgcccctc
    19981 gcccgatttt aaaacggaat cacaaagaat atgagttaag attttatctt ctgattgaat
    20041 caatactttt ctagaaccga tttcttttga atcgagagtt gttctttggg ccgtttcata
    20101 ggcacaatgg ataagaacaa aaattaagat taagaatagt tttttagtca tcactgcttt
    20161 cccgatggta agattcatct aaaagtggac ttggaaaaaa attgctttca gatgataaaa
    20221 tagatttgat ctttagtaca ttcgaaaacc ttattatatt atatgtcaac caaatctatc
    20281 attcagtata caaaaggtgc catgattgca atggcaaata ctcccaatcc aggttatgtt
    20341 catatattaa aatcgggaga gattgcaatc gaatctattt ttaagttttc atcaaaggat
    20401 ctcaatcgat atctacccgg agatacattt ggatatgtct cagccattac aaaaaatcca
    20461 catagcacaa ccttggtagc tgttaccgat tgtattgtaa tccgtttatc cgttgaaaat
    20521 ttttttgagt atttaaaaaa caatccagaa gtatttttaa aaattatatc ctataatgct
    20581 gaaaaattaa gagcctttgt aaatcatatc aatccttacc aaaaagatca aactccaact
    20641 actgactctc cagaaaaatt aattcacaat gcaaagatct acctagaaca caatgatata
    20701 aatcttgcct gtttctctct aaagaaatat ctagacggaa aatttgaaag agagaaagaa
    20761 acagaaaaaa tcaaggaagc agaggacttg ttacaaaaaa caaatccaaa atatactctc
    20821 ccagattacc caaattattc tgatgacaca gattttgttt taaaaaaagg agatatcgta
    20881 ttcgttgaag atgaagcaga agatgattat ttctatatcg ttaagcaagg ttcaataaaa
    20941 atatcaaaga ttgtagaagg aagagaattt atattgggga tactcggaaa aggagaaatc
    21001 ttcggagaaa tggcaattct gaaccaaaga tcgaggaatg caacggctat cgcatttgaa
    21061 gattgtgtaa ttcaaagatt aacagctaac acaattttag aaaaggtaga tgagacaatc
    21121 ctaactaaaa tttttcatat tatttcgcgc cgcctttggt atgcttttca aagagttttt
    21181 atgttaaagg taagtgatcc taacgttaaa ctatatattc aattgcagat gctgattgca
    21241 gatgaaatgt caaaatccga agtggatcga agtaaagaaa aatttgtatt tcgattctcc
    21301 ctccaggaat tattaaagat ggttgatata ctcgatatta gtagtgatag aatcagtgaa
    21361 tttttatcgg attctaacct atctttttcg catggtttag tggttgttaa agataagcag
    21421 atgctcgatt caaaagtgga cacagttaaa aaacgacata ctagactctt gaaagaaatt
    21481 atcatttaag taaaaaaata ttgtaaatag aactgtattt cgaatttgtg tagtgtatag
    21541 aatatggaga ataagagtat tttaactgat cccaaaatat ctgctcagca ggactctaaa
    21601 tcaaaagaag attccgcggc atatatccat atgccaacct attctaagat tacaaattta
    21661 ctcaaaagaa tattagattt agccagagat agacacagtc aaataaagag tcttaacgga
    21721 tcagaaagtg atgtaagcag tttactcagt ttagattatc tacatactct tttacaaaaa
    21781 gaaacatccc tcaaagaaac agaaaaaact cccgcttcca tcaatggagc tctcaagcag
    21841 atgcttgatt catatacaaa tggaaaagaa aaatatggtt acctttatcc aattttgatt
    21901 acagaaggga ataagataat tccaaaaatt attttaattg ctcctcaaaa agaaaaacaa
    21961 actccgattc aaccgtatag agaaagttgc tttcaatttt ctaaaaagat tattgatcaa
    22021 atgctcgtta caagagcaaa aaatacgaga cgatctgatg ataccactga tacatctttg
    22081 cttcgcttaa gtaagagtgg tagcgaatgg ctgtttccta aatttctttc tgttgaaagt
    22141 gagcttgtta gtctcctcaa aaaaggtttt caaccttatg cttatattcc tcttaatgat
    22201 tttattgagg atttcacaaa ctatggatta aagtctgata aattagttca gattttacca
    22261 gactatcata tgattcaaga aaaggtagag tttacggata acggtgagtt tgcaaaagtt
    22321 ccagaagtaa tttaccatta taaatcacaa gtagagagtt tagaaaaatt tgtgataaag
    22381 tatctaggta gctcagcaga aaaattaaaa tttaatctct atcaaacaag catccaacaa
    22441 ttcagagaaa aatacaatac tagttatcct gatgatccaa ctacaatcaa agaaagaatg
    22501 gatgcacttt ttgatttaat ttcactcttc cctggaacgg gagcacagga tgaaaatatt
    22561 aaaaagattt tagaaacttg tgatgaatcg attcaaattt gcaaaaaact ttatgccaga
    22621 atggatgatt tagttcgtta taaagaaaaa cacctttcga ccgttttttt aaatagtgtt
    22681 cagaaaaaaa ttgatgatca cacaaaagag aaaatgactc tctatatttt agatcttaaa
    22741 acaactttaa ataaatcaaa cattcaagac aaacaactgt tatacgaact ggaaaaagaa
    22801 ttaaaacaaa ttattcaaac taagtatggc tattttgaat cgatgagcaa tgagggaaga
    22861 gagtttttct atgtgactca tcctggttat atgacatgca cgattcacaa tctaacacgt
    22921 cttacaatag aaaataaatc ctatcaaaag caacttgata tcgctaaaat cattgcaaat
    22981 aaattggaag aagcaagaag aacagatatt gattctctcg ttacagaaga agacaaacaa
    23041 agactcctca aaaaaattga agaaataaga agccaagaaa cgcaaagact aaagagatta
    23101 gaattccgaa ataattataa tatttttgct ggtgtagctg gttttataat ttcactgatt
    23161 tattttttaa tcctttataa tacatataat gatttaatct atgcactcct cagtatacct
    23221 gttagtgtat ttacaggact tttattttct gtcctattta gaaaaaaacc ttttatagtt
    23281 agaaacgaaa aagaattaga agagaatgaa tcaagtgaaa gttctgctgg aagtgaaact
    23341 gaaaagacaa atcaaattat aaaagcatct gaatcctatg tatttaaatc taaattcaac
    23401 tcaatcgatg aaagaataca tgatagaaat agtttaaaag aaacgataaa agccaatctt
    23461 gatatcataa aggcgaattc acagattttt gcaaaagaaa aaaatgatga aaaaattatt
    23521 tcaacgattg aaaatgcaat gcttttctac tcagcagtga taactgttcc ttctgatatt
    23581 actccgaagg gtaaacctac catgatcatt ctacaacgat ctgatttaaa aaatcctcat
    23641 attagatcac aaattgcaga atattttaaa tttcaatccg cggcgggaag aagcacaggg
    23701 tccactgctt attataatta tctctaccaa gcagtagaga gagactactt taaatatgta
    23761 aaaggtagca acatccaaag acttaaaaaa gaattatagt tcttttaaaa aaatactctt
    23821 tcgaataaac tctctttcct cgtgcagaaa atgaattcca ttttctatgg aatagatttc
    23881 tttattgctc tggtagatcg atagtttcca tggcttttta tctaagatac aatgaacata
    23941 ggaaacaaac atctctgcaa aataatcaga ggcattggta gcggaataca tcgttggaaa
    24001 tctatatttt agtaagacag gatatatttt tttccaatcg gtatctaaat tgattttttt
    24061 atctggcgct gcataaaaag aaatattttt tctaaatgga aactcactac tatcataata
    24121 tgaatctgtt tctgttttcc atatcttatt taaaaatgaa tgattagaat agtctaatgt
    24181 tttgtttctt gcatctggcg ttatacggta tacgttagaa agaatatgac caatttcatg
    24241 cagtaaaata tacctaagag cattttctag agtattattt ttatcatctt ctatctgaat
    24301 ttttaattcg gtattacttt tatcaaagac tgaattttct ttaaatgtga tccaatcgtt
    24361 tgctttctgc tgaatgacat tgctatcaat gataatgatt ccaccaattg gtttattctc
    24421 ttcattatta taaatatatc ctgttaaccc agtccctcct agtttttcac aaaaataaat
    24481 accataaata tacttttcaa ataaaggctt tacctgagga tttaaatttt tagcaatttg
    24541 taaaatcaat gatttcattt gattcatgga ttttgtaggt tgaggaattt cactaaaccc
    24601 atcaattaga tttatctgtt taacataatc aattctaagt tcatctatat tatagattct
    24661 atcttcccaa actcctttct gattataatt atttaaatca gttgctggat gctttaataa
    24721 tacatccgca taactattat tttgcgctga accaatatta cattgaatgt cgaaaagaat
    24781 tataaataag agataaaata tagatttcat agcttatcta ataaaaagct attttataaa
    24841 aaggcaagta gtttattgtg aagagggaga ataaagcacc cttcgcaagc tcagggtgat
    24901 ttgtttttga aaacgcgatt tttctatttt taagcttcgt tttgtagttg gtctagcttc
    24961 atgctaacat cagtattcga tggattactt tttaggaaat cttttagaat tgagatagct
    25021 ttttcttttt cattcttaga ataaaaccag agatctgcta attcgataac aggtcttgga
    25081 ttgagtggat cctttctttt gagtaataag aaaatctcct gtgatttttc tgtttctccc
    25141 atgagattga gagtcgatgc tttaccaagt aatgcaaaat agtcttcatc tgaatttaag
    25201 attctattga aacattctaa tgctttttcg tattctccaa gcccacgcaa gctatccgca
    25261 taacggttta caatcaattt gttatttgga tcgaaatgaa gaatcttttc ccaataaatg
    25321 attgctttct taaattcttt tttcccacgg taggactcag caagaccata taaggcaaaa
    25381 aaattagttt catctaaaac tcttgctctc tcatagtatt caatggcacg atcaaagtct
    25441 tttatctttc gataactgtt tccaatttct gttagaattt taatattatt tggttgtgtt
    25501 gcaactaact tttcccacca tttaatcgca tcttggtatc tttgacaggc gaaatataag
    25561 tgacctattc caacaatcac atattgatct gtaggattaa tctgcaatgc agacatatag
    25621 taaatttccg actctttaaa gtttttcaat tttctatgtg cgtcagctac tctacttaag
    25681 atggatgcgt cagacatagt gatatgtcga tactcttctg caatttggat tacttgattc
    25741 agttgtttca tatcacgata acaattcata agtcccatga ttgaaaattt gttagaggta
    25801 tatagattca aacaattgcg atagtattta atcgcttctt cataattttg tgttttatat
    25861 aacaagtctc ccattccaac gagcccgtaa gtattgtttg gatctgttac taatagttct
    25921 gtgaattttt cttttgcgtc tttatatttc ctctgatcaa taagcttata tgcttctttc
    25981 gcaagtgtct ttatcttgtt aaagctttgg tcttcagtat ttaattctac atctatttca
    26041 ttcatattgt cagattattt ttcacgttta taaggtcaaa tttctatgga ttttgagaat
    26101 aaaataattc aagggaaata acggggaaaa tctatcctga aacatacggt tagcaagtga
    26161 ggtcttttgt tttttttaac aattctaaaa aattttctta acaatgaata tagttttaaa
    26221 aaactgtaat acatgagaaa acgccaaatt cttttcatct ttttattgat ctttattgtt
    26281 acttgtaagc aaaagaccaa agaaccagaa gctcaaattc ttggaacccg atattctaaa
    26341 attgatcaac acatcacctc tgtggctggc tccacaaaaa aagagcatag agttacattg
    26401 gtctatgcat tagaggaagt aaatgtttta gaaaagattt cccaagacaa taaagacttc
    26461 tttaaacttt ctactgttac tggtaaagaa ggttatgcgc aagcgtcaaa tttttctgaa
    26521 gcagttttat ttgtagttaa ggaagggtta agcgcattta gaaagcctac tctaacagca
    26581 ggaacaaaag gaagattttc tatcggagcg atatgcttcg taaaagaaat tcaaggagaa
    26641 tggtctaacg tagattgtat cacagcaaat gtgaaagatg gagtcattga agattggttt
    26701 gatgtttgga ttcagtctgc agatgaaaga ctttctaaag atcctatgct cggacaaacg
    26761 gctctactca ttcgagacgc atataaaact atcaacaagg caaataagct gaccggaacc
    26821 gatagagaaa aagcaatgat tgatgcaaaa gataaattag caaaagcaag cgagaaagaa
    26881 gatattctac aaccagtggt tacgtctatt atggttaaat atggaatgtt agatgagcct
    26941 ccagctactc caacaaaacc acccgaggaa actcaatctc caaataatac gcaatagaat
    27001 ttttaaaagg aaaaaacaat gtcaggaaca gagaaaaaaa ttacaggtgc aagattactt
    27061 atcgaactca tggaagaagc tggaatcgaa gtttgcttcg gttatcctgg gggagccatt
    27121 ttacctttct atgacgaatt atacaagagt aaaattaaac attatttagt tagacatgaa
    27181 caaggcgcaa ttcacatggc tgaaggctat gcaagagcaa caggcaaaat tggagtctgc
    27241 attgccactt ctggtccagg cgctacaaat ctagtaacag gacttgctga cgcaaaaatg
    27301 gattctatcc caattcttgc aattacaggt caagtagcaa ccagtgctat aggaactgac
    27361 gcttttcaag aggctgatat ttttggaatc acaatcccaa tcacaaaata caatgcactg
    27421 atgcgaagtg cagatgacat tgcaagacat tttgaagaag cttacaaaat agcattaggc
    27481 ggaagacccg gacctgtttt acttgatttc ccaaaagatg tgcaaactca acttacatct
    27541 gttagaaagg ctcctaaact aaagatcgca aagcatcatt atacaaatcc agaaattaaa
    27601 ggagatgtaa aagcctttgc agaagcaatc aacaatgcaa agcgaccact tctttatgta
    27661 ggcggtggtg cgatcaatgc aaatgcagcc attgaaatta aagaactcgc tctagcgggt
    27721 aatataccag taaccacaac tcttatggga attggttctt ttcctggcaa ccatccaaat
    27781 tcactcggaa tgcttggaat gcacggaaca gcctatgcaa ataaagcagt tttggattgt
    27841 gactatattt tgaacctagg tgcaagattc gatgataggg ttgctaaaat tggcgaattt
    27901 gcggaaaatg ctgtgagagc tcatattgat attgatacag ctgaatttaa taaacggatc
    27961 gatgtagact atgtattaca cggtgattta aaagatgcga tcaaggctat tattcctcac
    28021 atcaaaaaag tagataggac agactggtta aatcatatac aaaaactaaa agaaaatcac
    28081 ccattggaat atgataacac agcggataat caaattaagc cccaggattt tctagacaga
    28141 ctatataaaa agacaaaggg gcaatgcatt gtcacgacag atgttggaca gcatcaaatg
    28201 tgggcagcgc agtattatct attcgaagag ccaaacaatt ggttaacctc tggagggctt
    28261 ggaactatgg gttacggact tccagcagca attggagcaa aaattggaag acctgataaa
    28321 attgtaatct gtgtatcggg tgacggttcg atccaaatga atattcaaga actagcaacg
    28381 atcacgatgt ataaagtagg tgtaaagatt ttaatcttta acaataactt tcttggaatg
    28441 gtgcgtcagt ggcaagagtt attctatgaa gaaagattct ctcattcaga atggaactac
    28501 aatccaaatt ttgtgaaagt tgcagatggg tatgacattc cagccatgtc catttctaaa
    28561 aaagaagata tagataaagc attagaattc tttttgaaag atgataaatc tgcacttcta
    28621 gaagttatgg ttccagcgga agaaaaagta tttccaatga ttccggcagg caaatcacaa
    28681 aaagagatgt tagagtttaa agacttagca aaattaaagg cagcaaaatg aaacacactt
    28741 taaatatatt agtaaataat catccgggag taatgagtca tgtatcagga ctctttacta
    28801 gaagaagtta caatattgat tctattgcag tcggtgtgac cgaaaatcct gaagtatcta
    28861 gcatgactat cgtcttaaga ggagatgatt caatcgtaga tcaagtcaaa aaacaacttc
    28921 tgaaattgcc ggatgtatta aaggtcacag atcttttcta catggaagca attactcgtg
    28981 aattggtttt agtcaaagta atggcagatg ataacagtag aaccgaaatc atttctctat
    29041 gtggagtctt tggtgcaaag attgttgacg ttacagattc tagtctcatg gttgaattct
    29101 ccggaaatgc gcgccaggta acagccttta tcgcgatgat ggcaaaacat ggaattattg
    29161 aaatggcgag aacaggacaa attgcgcttg cttgtcagag tggctcgtag gaattcttca
    29221 tcttacatct atttgccgca aataaccaaa ctactagcaa ctaaaggaaa aataaattta
    29281 tagatatcaa tgatcttcat tttttacaaa tgataatcat tgatggaaaa gaatactaac
    29341 gatgaattgg aggaagagtt ctactagact ccccgcatca ctccgattaa agatgcgatc
    29401 attcaaaatt atctatcgtc tctgtatctc tctctgatcg gcgccctcgg tggtaattct
    29461 ttttttgcta ttactcaagg cttttctaca atccaaatcc tatttttata ttcatctaga
    29521 taggaagcat atcttccctt ttcaaaatag gattctaaaa caagtggcga caaatccaaa
    29581 ttatccacat ctagggacgg aacagtaaat gtaactgttc ctggagttac gaaacaatga
    29641 acagagggtc tatcaatgaa gacagtaatt ccagattccg ttttcaattc agcatgattc
    29701 tcagatgcaa caagaataga aagtgaaaat aaatccttat aaatctttgc cgttttaata
    29761 caatcgagtg aataaagatt cgctccgtaa aattgaaaag cattcattgt tttttccatt
    29821 caaatctata gtctttccga aagatcgttc tgtatccttg gtgtgaataa attcgaaagc
    29881 tcagatcctt tcggtgatat tctaaacctt attcagaatc gaatcgcact tccacgaact
    29941 agcaaaagtc acagtaaaaa ccttttttct tacttgactt tcggctttct aaaattatac
    30001 ttattctatt actaaaaaaa actatgaaaa aattaacgcg ccacagaaaa attattttag
    30061 aaaatatgca atccagaaag gatcatccaa ccgccaagat ggtatatgac tcaattcgct
    30121 ccaatgtaaa aagcctgagt ttcgcaactg tatacaattc tttagaatac ttatataaca
    30181 atggctatat aaaaaaatta gactcctttt cagattcagc tcattatgat gcagtcttag
    30241 acaaacactc tcatcttatt tgtagacatt gtggaaaaat tattgatttt ccaaatattg
    30301 acattactga caaactctct tctttgactg gaatttttga agcagaagat gtagccgtta
    30361 cggttcgagg attttgccaa acctgtttag ataaaaaata attacatggg tataatccac
    30421 gagttagatg taaatctaat caataaaatt gcggctggag aagtcatcga atcagcccac
    30481 tctgttatca aagaattact ggaaaactcg attgatgcaa aatcaaatca gattgatatt
    30541 caaacagaat caggaggact ctctcaaatc ctcgtttccg ataatggaac aggaattgca
    30601 gaagaagacc tccctctagc cgttaagcgc cacgcaacta gtaaaataaa aaatttagac
    30661 gatttagaaa cagttttaac ttatggattc agaggagagg ctttatcatc tatcgcttct
    30721 gtctctaaat taaaattaac aacaggaact agccaaaatt cacccgccat tgagattctt
    30781 tgcgacaaag gagaaatctt aaacaagaaa aatacgactg gcttcaaagg aactaaaatt
    30841 gaagtagaag atttattcta taatactcca gttagaagaa aatttctaaa atcggaaaaa
    30901 gcggaagata agaaaataaa agacagaatc gcgatcaccg ctatcgcaaa cccgaatatc
    30961 ggatttaggt atatccaaaa tcaaaaggaa atatttaatc taaaaccaga atcaacaaga
    31021 gaaagaattg tatctgtttt tggagataat ttagaaaacc atttactgga agtaaattca
    31081 gaaaagaaag gaatcaaagg ttacgggttt atcagcgatc cggaatttta caaatccaat
    31141 cgcagtggac aattcttatt tgtaaatggt agacctattg aaatgcgtta tagctcttat
    31201 ctattaaaaa aagcctatga cgaattgatt cctacacaag ctcatccttg gtgttttata
    31261 ttttttgaaa tagatccaaa gcatatagat gtaaatgttc atccgaccaa aaaagaaatt
    31321 cgttttttag atgaagaagg atttaactct ttctttttac aaatgattca tgccgaattg
    31381 aattccaaaa cacctgttgg aattttagaa atgagaaaac gatttttcaa tgaaggaaaa
    31441 agagcagaag caattgcaag ggctaatagc ttattatccc ctactttctt ttctacgaat
    31501 acaaattcac agtctagaga cacacaagaa attttacatg actctcttta tgaagaagta
    31561 aaagaacaag tcccattctc agtcgagact gttggagcgg gaaccaattt acacgagctt
    31621 actcaaacac cagaaagaaa aagagaattt atccccaaaa aacattttgg aattattttt
    31681 gaaacgttca tcttggcgga agcagaggat ggaatctata tcatagacca acacaccgct
    31741 catgaaagaa ttcgatacga agaagtatta aaccaactca aagaaaaaaa cttaacccaa
    31801 caattactca ctccaattcg gattgatcta gcaaagtcag acgcagaaga aattctagag
    31861 agaaaagatg aatacgctaa agttgggatt acgttagatg atttaggtgg tggaaccatt
    31921 ttagtaagag agattccagt ctttctcgat ccaggaaaag aaaaagaaac cattttagat
    31981 ttcttaaatc gcacaaaagg agaaaaggtt ttagaaaaag aactctatga tttaatggca
    32041 aaatgtgtcg catgtagatc agcaattaaa aaaggggatc atatgtctga tcatatttta
    32101 ggagaaatcc taaatcgact tagttattgt gaaaatcctt ctagatgtcc acatgggaga
    32161 ccgacattaa taaaactaac acgcgacgat ttagagaaaa tgtttcatag gaaataaatg
    32221 gataaagatt tacgaaaact aatcattcaa tccgtcattg ggattgtatt aatcatagca
    32281 cttgtcatac tactagcgca cattgtaaaa ggaccaatca ctcagttttc aaaactattt
    32341 gtagactacc ttggagtctg gggagttggt ttaggaattc tagtgagtga ttcccttcct
    32401 gcatttatgg ttccagatgc ctttctagtt tttgcagtag ctggtaagtt aggagacttt
    32461 gaagtaatct tattttgctc tatcggtagc ttaattggtg gctctaattc ctatgcgatc
    32521 ggtaggtatc tcatacctag atttgaatcg ggtagaaatc taataaaaaa acatgaagca
    32581 aaacttcttc cctatatcga aaaatatgga atgcttgcag tagtcattgc ggcaacaact
    32641 ccacttccct attcttggat gagtatacta gtgggaagtt ttaaaatgtc tttttcaaaa
    32701 tttttattgt gtagcctaac acgaattcct cgctttgctg tttattatta tgcgattaaa
    32761 tttggctggg tagaagacat tgtatttttt ctttctcaaa tttattttac ctaaattaac
    32821 gtaagttcga ccaaagaatt tttctccatc aatggtaata aatttactcc tttgtatcgg
    32881 tgctctcggt gttcacacat tcagaattct cagtgtaaat cggtgataat cttttaaatt
    32941 ttctgttaca tcgaactcgt gttaaattgc aataaatcta aatcagtata aaaattctgt
    33001 tgaattcttt ttggattcca gcaaagtaac agtcatagta aggttaaatc aacgcgaatt
    33061 cgatataaga atatttatcc acagatagac acggatgaac acagaaaaag gcggatggtt
    33121 ttaattatta ttattttatt cgtgtctatc agtgttcatc tgtggtaatc ttatgaattt
    33181 tttgttacat cgaactggcg ttatatcagt actaaacttt tttagtagaa caggcaaaac
    33241 acaatgaaaa ccaagtttga attctttgaa atcgaaatag aagatcatat cgcaattgtt
    33301 tatttaaata aaccagaaaa aagaaatgca atggactgga gtttctggag agacctacca
    33361 gaagttgtag aggaaatcaa taaagacgaa accattcgag cttttatcat tgcagctcgc
    33421 ggcaaatctt tttcaacagg actcgatgta ttaagtttca cttcccaagt agaggaattt
    33481 ttagttccaa ataatgcaga tacaagaaaa cgtttttata atcatattca agtgatgcaa
    33541 agtggaatca acgcagttta caattcaaac aaaccttcta ttgcagtcgt gcaaaagcat
    33601 tgtatcggag gagctttaga tttaatctct gcctgcgata tacgttattg cacaacggac
    33661 gcaagctttt ctttaagaga agtaaaagtg gcaattgttg ctgatatggg aagtatcaat
    33721 cgacttcctg ctattattgg ccaaggttac acaagagaac tcgcattgac tggtcgagat
    33781 gtaagtgcag aagaagcaag caagatgaat cttgtcaacg gagtctatcc aactcttgaa
    33841 gaagcaatga aaaaagcaaa agatgtagca aaagacattg cctcctcttc tactcttgta
    33901 gtagaaggag tgaaggaagt tatgcgcttt agtgaaggca aaccaatgga agctggactc
    33961 aattatgtat gtgtctggaa ttcaagtttt ctggcatcca gtgattttga tgcagcaaga
    34021 aaagcatttg ctactaagac aaaaccagtc tacaacgaaa ataagaaatg agttttatat
    34081 ccattctaag acaagcttta tacgaagaaa gtatgaagca ttactcaaga gcgtttaata
    34141 gctatcataa agcaattgga cttactaaaa atccacgcac gattgcgcga ctaacggctc
    34201 gtaaaggctg gtgccagcag tatgtaggaa accaagatca agcgcaacaa actttctctg
    34261 atcttgccaa atccgcagaa gttccagaag cgtatttatt aacctctatc tattatctaa
    34321 aaacagatag aatgaaatca gcaaaaaata tgctaaacaa aggaatagaa aaattccctg
    34381 agtatttaga actctatctc acacttgcct ctttattaaa agataccgaa cgagcaaatg
    34441 aatccattga ggttctaaag aaagcacttt cccaagaaaa actaactaga gggaaaggtg
    34501 gaattgaaag aaaagacatt tgggcagaac ttggaaatct ctactatgaa agaggaagtt
    34561 ataattcttg tattgtttgc ctgaaacggt ctctcaaaat gggaacagat aaaaactttt
    34621 tgcattatgc tttgatttca aaatcctatt taaaattaaa cgatccactc aatgctttaa
    34681 aatacattga tttacaccta tcgtattttg aagacttaga tcctgaagat tttattacaa
    34741 aagcgagagc acatgcaaga cttggagata atcatctcgc aggtgcaaat ctactacaag
    34801 cctatgattc agatggattc ttgagactca aaacagaaga aatggcagat ttttcggctc
    34861 ttgtgcaaag tggttttttt acgactttag agaatttaga aatctattga tttcccttcc
    34921 taaaaagaag ttaaaacagt ccttctctat ttctaaagaa ctattgggaa tttaaaaata
    34981 ggaaatcaaa agaaaccaaa agataatttc aaagtcttag aatgaatcgc atatgttttg
    35041 ttttctatgc ttaaatgaga gcaatttctc atttcatcct actcttaatt tatctttagg
    35101 gatcgtttgc tgtgacttat tcatcatttt acttaatttg cctaggaaat gaattgcctt
    35161 aaagttttac agtttttaga atggaatgaa tttaataata tgaatgttat agaggagatt
    35221 acatgaagaa acttatttct ttattcgtta gtctgatttt attctattgc gcgagtcccg
    35281 accaaaaagg ggacgggttt atctatgtgc aaaatatcac tggaatcaat ctagatgatg
    35341 ctgagttaaa tgcgaagcgt gcaattgtag caaaaggtct tggtgaatta gtggaaggtc
    35401 gaactgacgt aaaccaagga caacttatgg aatctgttgt aaacacatct gtagaaggat
    35461 ttgttttaga ttatcaacaa attggaaaag acagaaaaaa aggtgcgtta gttgaaattg
    35521 acgcaaaggg aaaagtagat agaggaacag ctcaaagcat aattgataaa ctattatctc
    35581 aagccggaaa cccaaacttt cttatgttag ttgatgaaga gattgacaag aagaagagtc
    35641 cacccactgc cattgcaaca gagaattcaa tcgtaaaaaa cttttctgaa ttcaattttc
    35701 tagatagaga gcaattccgt agagttcttg caaaagaagg cggaaaaaca atcggagttt
    35761 atggtgatcc tagtgcagaa gcaaaagcaa ctgaggtagc tgctcaacaa ggtgctgaaa
    35821 ttttatttat agggcaaacc tctgttaaaa acaacggacc tattatggat tccggaatga
    35881 tgtctatgca agcgacagtc cgattcaaaa ttgtagatgt tggttctgct aagatcattg
    35941 cagcagataa cgcaggtgga gcagcacctc atgtggacgt agacagagga gcacaagaag
    36001 caattgataa agctctagtg caatcaaaac ctaagattct agatcaaatc agaaccaaat
    36061 ggaagcgtgg tatgaccatt cgtctcgtga ttgatggaat gaactacgat gatttcgtag
    36121 acaaaggcat tacaggcacg attcgaagta tgaaaggtgt taatgctgta aacgaaaaag
    36181 gaagttctaa ttcaaacaat tctatcgtat tagaagtgca agcagacttc aacggcattg
    36241 tgctttatag aagaatgcga gaaagaagag aagacctcgg aattaaattc gaacaaaaag
    36301 aagtaaaacc gggtaatgtc caaatcaaag taaacaaata atacgaagag cggctaatgc
    36361 agccaatttt ttctcgttag ccgcttttta cgatttacaa aaaaactatt aaaagcgaaa
    36421 agtaaacatt gtaattctag agagaaatta aatccagatc aatcaacaaa ggaacataga
    36481 tccatatgtt aaaaacaata tttcctattt cgtttagcaa tctacctact ccaattaaaa
    36541 tcattggaac ttatttctta atcacaccga ttgtgagtta cattgcgact gcgtataaac
    36601 tcgatttgcc attatccaat gtaaaaatga ttttagcaag ttttgataaa ttaaatatta
    36661 tactaaactt aatgggcttt cttgttggga ttggaatctt aggcgttaaa aaatggggtt
    36721 attttgtttt tcttaccttt aactcagctc ttattattca tgggatttat cttttagttc
    36781 gtttcaatgg tattgaaaca gaagtgatga gccaagaaag acttatttta aatttaatca
    36841 tcacaattct acctttcttt ttaattctct attttttaaa ttctgaaatt tctacacctt
    36901 acttaactct tgtgcctcgc ggttttagaa aaaaatggag aacagaaatt ccaatcaaag
    36961 gatttctact cgattcactt ggaaacaagc taaatattac aacgttagac gttagcccaa
    37021 ctgggtgtct tgcagaagta gatggataca ttgcagatga agatgacttc gcactcacat
    37081 ttgatttaga gaaagactgg agagtgagcg caagtgtagt tcgcattcaa aatgaaaatg
    37141 taggacttcg cttcaattac aatggaacaa atgacccagc caaaaaagaa ttaaagaatt
    37201 atctagaaag taaactccat ccaaggtatg atataacaat tccttgtgat tggtctgtca
    37261 acggacaaaa ttatacaagt gatatcttaa atatttcaga gggtggactc tttctatcaa
    37321 ccaatgaatc tgctaaaata ggagataacg tcagtttctc ttttaaacta ctaggctttc
    37381 gtttttcagg aacaggttct atcagctgga tcaactcttc tgcgaaatac aataaacctg
    37441 ttggaattgg aatctctttc aatggcttaa agaaaaatat tctatataat tttattatct
    37501 caggatttcg cttatttgct ggctttcagt ctagagagag atagattttt atcacagagt
    37561 aagggcaggt gaaagatctg cccctacaat cattttcaaa aatttcaagt tacttggaac
    37621 tcaaataact tctaacaaca gtcgcaatca gactttattc taagatttta tctttgacaa
    37681 aaaaaatgaa atggatatcg tatacttatg gaatcagcaa ttctatctaa tccaaagttc
    37741 atttacaaag aaggtaaacc agtagaagta attttggata ttgaaactta cacaaaaatt
    37801 ttagatattc ttgaaagaaa agataagaaa aaaaaacaaa tcattgttga aatgaatgga
    37861 gctacactca tcagaaatag tgaagcaaag actggcaaag acatagctaa atttgctgga
    37921 atatggaaag aaagaaacga aattaaaaac tcgctagact acgctagagc acttagaaaa
    37981 aaagctcatc aaagaccttc cattcaagaa gcaataaaat ctttaaacaa atgatatttt
    38041 tggattcgga tataattatc gatatctggc gaagtttcca gccatccgtt cattggattg
    38101 aaaatgtcaa agaagaagtt attatatcag ggttttctta tttagaatta ttgtaaggtt
    38161 gtgagaatca aaaatcaata gataaagtaa gtaaagcgtt aacttcattt ggtattatat
    38221 ggccttcttc cgaaacaatg aatcaagcac tggaaacata ttctgctgta aaacttaaat
    38281 caggaattga ttttattgac tgtttaatag gtcatactgc aatttcatta aaaattcctt
    38341 tatatacttt taacgaaaaa cattttaaat tcatacctaa tttaaaaatt gtaaaaccct
    38401 ataaaaaatg ataacgtttt aataaattaa cattatggtt aagtccatat ctataccata
    38461 acgtcttaaa aacaaacggt gaaacttcct aaacttaagc aaagctgcag agttagaaaa
    38521 ataaaatgca tcaaacaacc atcggtgcgt atcggtggta aaaaaattcc atcttctatc
    38581 tttcttcgtg ttaccttcgt attaacgata cactatctgg ctactatctc gcataccaac
    38641 tgtcttcccc ctagcataac gcaagccagc atctagccag gggatagctt cttggtctcc
    38701 tagatggtag ccagatagta taacgttatc tataccttaa tacaaagaga cattaaaaga
    38761 ttaccaccga taaacaccga tgggcaccga taaaagatac gatgtttttt tggaatcgat
    38821 agtctaatgg ttgtatgcct ctgcaagcat gggttatcgt attacattat gcaaccaaaa
    38881 cgagtatgat aaaaatcata aaataaattt taaaatatca cctattcggg tgattgacaa
    38941 tattacgatt gtaagatatg cgtctaaact aacgttttat taaaaaatta ctctctaaat
    39001 ttttttcttg caagttcatt tgttttaatc ttagttggtt ttcaagaaag agaatgatat
    39061 gtttatagca aaacagcaaa acagcaaaac agcaaaaagg aagaaatact tcttttaagc
    39121 tgcaaatttt ccaattttat aattgtttca aatcttcttc ttccaaaaat ccctttaatt
    39181 tttctcattc tacctgtctt gtttggatac ggattccgaa aacggaaatc ctctcatctg
    39241 ctccactcca aaggtttgtt atgattgtat atgctttgct atcaaaagta attttgagga
    39301 ataagaaatg tcagtaacga atctattaat cacaatacat gggattaatc cttgtttgga
    39361 gccgaaagat ttaaccgaag actttaaaaa actctttgat tcgatggtag cccaacgacc
    39421 tatgttaaat aatttgtttc catcggatca tcggatttat atacagtggg gtcatccacc
    39481 aaatgaaaat tcaaaagaaa atatttttac gattggaagg gctcaatcta aaattcttaa
    39541 gctgttagat tatgataagt ctgaacgtac taataacgat ataaaattaa ataatttttt
    39601 tcccaacaac ctaaatgttt cttatatttt atccaaactt ttgagggaga actttgttct
    39661 tactggtctt ggagatgccg cctattattg ttccgaggaa ggagagaacg aagtaagatc
    39721 ttacatatat gaaataattc taagaaaatt ggaagaatta aaaaagaaga actctagaat
    39781 tagaatacat ttcatcggtc acagtctggg agttgccatt tgccacgata ttctatttgg
    39841 cttgtcttat aatggtcaaa aatacgaacc gggattttta aaaaatgcat tgggcaagga
    39901 agatttgcta aagctattca aattctggag acaggaaatg ctttctcatg ctaaactaga
    39961 agctggttcg tttatatcac tcgcttctca attgccatta ttcatccaaa gaaaacatga
    40021 cattttaaaa aagtttatca atgaagaaaa ttataaatta gatttaactg atattggaat
    40081 ttctggaact caattgaagt ggttactttt ttatgatcta aatgatttac ttggatttcg
    40141 tacgagacca ctctatcatg atccaaataa catcgtaaaa gaaattgaag tggactgtgg
    40201 tgttttaccg gacagcgccc atcttaacta tttcaagaat ccaactgtta tttgggaatc
    40261 agcaaaattg ttagaaatga atggaaggga ttaatgatta atttcaatct tgatgctttt
    40321 actaaatatg ttgacttact aaaatttatc gttgagttta tagcattact cattggttct
    40381 ggtttcggct tttatcgttt tattttaaaa aaagaatttc atcccaaaat tcaattcagc
    40441 gtagaattaa acgtgattga tcgaacaagt aaaggatggc ttgtagaggt aatagcgttc
    40501 atagaaaata aagggagcgt taggcactta attgatacca atgattttac ctttactatt
    40561 cgttatctta caaaaaaatc aaatttttct aatgatataa caatccaaaa agaaaaattt
    40621 tatacactga actttgataa taaagttgag gatagcacaa acaaaggtta ttttcaaatg
    40681 ctaccaaatt tttttggtga gacatttata gatccaagtg taaaacaaaa atatagctgt
    40741 cctctcgtaa ttctaaacga tgcaactcac gttttagtta tatcgaagtt cagatacaaa
    40801 gatttaagta tggattttca tacggcacaa ggattttaca aattagaaac aaatgcgaaa
    40861 attgaacaaa aacaaaatca aggagattga ctatgagaaa gtcagtaagt attttattag
    40921 tattggttgc actggcaatg atcgggtgtg cgagcgggga ttcgggtaat agcagtgaac
    40981 cagtaagaga gaattcccaa aagtcagatg gagtggaatc cgaaaaacaa agaggaattg
    41041 attggataaa agaaaactat atcgacaaat ttgagcaagc tcctgtttta aagatgtttg
    41101 ataaacacac tagtgctgga aaaaattata tattagtttt cgggggtaag ttgagtaaaa
    41161 caaaaaaggc aacagaattt tattggagaa ataatacctt tactactcgt gagttaagtg
    41221 agcctgaaat gaagagaatg gatttgtgta atggttgcac caagatattt gaatagttta
    41281 tgtatgtgag atttcttttt ttccttgttt tattggttct tttgacctgc aatacgcctg
    41341 agcaaaagac ttttaaacca gttgtcgagt cagctaaaat caaagggcga attcagatag
    41401 ctgaaattaa atatacaggt ccaccagctc aga
//
LOCUS       DAOQOS010000043        40903 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_389_length_40903_cov_6.834655, whole genome shotgun sequence.
ACCESSION   DAOQOS010000043 DAOQOS010000000
VERSION     DAOQOS010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 40903)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 40903)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40903
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_389_length_40903_cov_6.834655"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..469)
                     /locus_tag="PLF66_11255"
     CDS             complement(<1..469)
                     /locus_tag="PLF66_11255"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HMZ64665.1"
                     /translation="MAEELPPIDPAEFKKVVETRRSIRLYTEETIPEDVMRQCLDLAL
                     LAPSSFNLQPWEFYWVRSPEKKKKLVEACLSQSTAKTAAELVVCVARRKTWRKNRKEI
                     LERIESENLNLPESHIQYYKILIPFFYTQGFLDTIGFLKSILYYIRGLFVPTPR"
     gene            complement(472..2163)
                     /locus_tag="PLF66_11260"
     CDS             complement(472..2163)
                     /locus_tag="PLF66_11260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020777996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HMZ64666.1"
                     /translation="MSFSKYLILFLISLIPYVLTLTHDVIDIDSSQYAEIAREMIETG
                     EFFHLKDNGKNYLDKPILTFWTIASSYKIFGISNFAYRLPAFLITLLSFYSIFRITTL
                     ISGSRERGILASLAYSVAPGVFAMLIDPKIDVYLVAYLTFAFHAYYLGVKQNSNWFYL
                     MYLFISMGFITKGPISMVIPALAIGGDILVRRDWKLLLRMKVLSGILIVASLPAFWSY
                     LLYQDFSSHGPVFFLWIQSFGRFYRQMYNQKFDPLYFVTNYSWAFAGFILPIFYTMFL
                     FFKNIKQNKSDVKLFRRILNSIEENKEKESYYVIYIWLFVFLFLISFSRYQLPQYIYW
                     ALPAGAIFISRYIEEFLNIKKETNLSRSVFQNLLFVIPALLIIAIIVIPLVVLNVNIL
                     YGIPILVFLVALLYFKKILPVSFIVSVLSLSLFFSIVGLYIYPELTSYQPSNQFGAVI
                     RQMEPDKNEIFTYRMSFSKRSYAFYAARFTKPIFDREKFFKVLESESERLVVVSKDDL
                     PQFQDFLKENVLIEVIAEKPSYKVATPKADFFLKEKRPLVTKNILLIKVKYVKGT"
     gene            complement(2174..2470)
                     /locus_tag="PLF66_11265"
     CDS             complement(2174..2470)
                     /locus_tag="PLF66_11265"
                     /inference="COORDINATES: protein motif:HMM:TIGR04453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-rich protein"
                     /protein_id="HMZ64667.1"
                     /translation="MQNRIQFFIPLFFTIVFLFFTNCKDPVEEKCTQACGFFVSCTEK
                     AQKMKIEGELLKQATIQCIDGCTRFQSQILSCYDAESNSCNGMAECMKQSGLDE"
     gene            complement(2743..3552)
                     /locus_tag="PLF66_11270"
     CDS             complement(2743..3552)
                     /locus_tag="PLF66_11270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016546705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_component: GO:0035632 - mitochondrial prohibitin
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HMZ64668.1"
                     /translation="MAEKVININQFGGMNLYTKIGLGVAAIVLLLIVNPCVIVSPGHK
                     GVVLNLGAVSEKVLDEGVHFRMPLVQSVIEIDTRVLKEETKAEAASKDLQNISTVIAV
                     NYHLKPDMVNDLYRTIGLNYENVIIDPAIQEVLKGVTAKYTAAELITLREQVSHNIKS
                     ALESRLEKYHIVIDDFSIKDFQFSATFAKAIESKQEAEQLALKAERDLQRIKIEAEQQ
                     IATAKAEAETLRLKNGSVTPLMIQLNAIDKWDGKLPQYMLGNNSLPFVNIK"
     gene            complement(3604..4755)
                     /locus_tag="PLF66_11275"
     CDS             complement(3604..4755)
                     /locus_tag="PLF66_11275"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HMZ64669.1"
                     /translation="MFHKYLPLLTFILKKLTFSRLHLVSFFLALITFCNLPLRPEFGS
                     SGSVVLKQKGEINFGRLANAPFKFSTSSCISDSNCGQNLFDSNSNTFWVTDKKLENEW
                     VIIDFGSKRLLTGVETEFAFMTQTSYEIQVLNREVWTTIYTNNKPDKKNRDTLLGIDA
                     STVRILFNKTSDSSISLANLRLLLGETNLTGIDSRLTGFAFPIENGVMPTDDYSLPGA
                     PRKYRNGVHKGLDISSKLNFFQVNTNVSRDTKIISANDGVVIRADLNYKPMTESEFKE
                     ISAYNQTHPVTYVDRDFGGRQVWIEHKGGIITTYNHLSAIQSGITVGAKVRKGDTIGY
                     AGNSGLLGEARNNNDAIHLHFEIWIDGEFLGNDMTLPQIRKLLQYFFSE"
     gene            complement(4795..5175)
                     /locus_tag="PLF66_11280"
     CDS             complement(4795..5175)
                     /locus_tag="PLF66_11280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020777025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64670.1"
                     /translation="MDSKERAALIREGNAAFNDGDYPKARELYLKCDYKDGLIRLGDY
                     YMYEKKLPILAYGYYKKAGHTQKIDEIFQRMLYALGQWIGMDKFKIKEAPQTNDKPLS
                     PDDFKVHPILKAKALEILQKSGNK"
     gene            complement(5231..7453)
                     /gene="asd"
                     /locus_tag="PLF66_11285"
     CDS             complement(5231..7453)
                     /gene="asd"
                     /locus_tag="PLF66_11285"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000938814.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HMZ64671.1"
                     /translation="MNKENFIFFGDEIIASYCGLLIFAAIYITYKLKFPQVRFLFLAL
                     KILSGAMDHKGSKGQIVHSQAFSAGTASSLLPGAILGSALAMMIGGVGALFWIWVGSF
                     LIMPLRAVSSTLAIRFRTKLPNGRYLAGPMYFIEKALKARWLAIAFSIGCILTVLSLG
                     GVIPVLSMTYVAKEAFDAKGLGLSIALIAIVIFIVLGGIRRIGRVSGVIVPTGLILFF
                     GFYFLIFNETLIPFTQFIGVVFHNAFNFEAIGVGSLLSILVYIGQSSGVFFLSTEMGL
                     GKSAGISGAVRTDHAFKQGLVSMLSAFFEGFIVSTLIFYLLFSANAVGLEDQMFFLKR
                     VLHHGKDPYYIIFYFSFFLFCIASIIGWFYTGEQSAYYILGEKFANLFRIVFFGVVIS
                     SSYLFLREGNYFIELALNAGYTMALFTAIPILISLILLVKMVGSEMNKYLTEGGVRYE
                     IFKDIYLLILSALPKNLISKLFGSFTYLRLPRFMMVPILKAFAKAYKINVSEAELNIK
                     EYNSLNQFFTRALKAGARIIDSAENAVVSPVDARITSFGDINDDTLIQAKGFDYSVKE
                     LLGSEKYYPFFNNGKFITFYLSPQDYHRIHSPFYGKILGYYYAPGKLFPVNDAAVGGI
                     RGLFPKNERLITFLQTEYGKIAIVKVGASNVGKIRVTYDTKIVTNKLIRFPKEHEYTN
                     VNILIDKGSELGRFEMGSTVILVFENNTVELNPFTKDERQTYGSTVGFFKEKILSLPK
                     "
     gene            complement(7450..8937)
                     /locus_tag="PLF66_11290"
     CDS             complement(7450..8937)
                     /locus_tag="PLF66_11290"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020776189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HMZ64672.1"
                     /translation="MKLPSSKLKMYVGPNKSKSIYKVQHIFKDKVPNSIKKALQFEMD
                     AKMFQADAGQQFINHNDQIIYIGLGEESKLTLRKLASYYFKLGDTFIKWYDIGLEIHI
                     SKELSEMFSTFNLVYQLANSLEIIAFPVDSLSKTFRDRKIEVGDISFVIENVKEEKFA
                     KDALEKSKIVSKYLNESRYVEHLPANHFTPEEFVSRSKEIAKENKLKITVFDEDRLEK
                     ENFGGILSVCRGSDKKPKMIVLEYSPSNVKTKKTLALVGKGLTFDSGGISIKPSAEMH
                     EMKYDMCGAAAVIHAIGAIAALGIKVRVIAAIGVAENMPDAAATKPGDVYTAYNGTTI
                     EVQNTDAEGRLVLGDVISYVCKKYRPEYMVDLATLTGAVIIALGHEAAGVMTNSRHLY
                     DLIDSASTSSEDRVWQLPLWEEYGEDLKSDIADFRNITGGRAAGTLSAAQFLANFVEG
                     DVKWAHMDIAGTAWRSKASGTQINGPTGYGIRLLVDLAKEIENKP"
     gene            complement(8934..9410)
                     /gene="bcp"
                     /locus_tag="PLF66_11295"
     CDS             complement(8934..9410)
                     /gene="bcp"
                     /locus_tag="PLF66_11295"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017851549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HMZ64673.1"
                     /translation="MTNLEVGKKIPAFSALNAEGKKVKLSDITGKNGLVLYFYPKDNT
                     PGCTTEACDFRDNLEGIKKLGYSVVGVSKDDLKSHKKFIEKQDLNFELLSDAESDMAE
                     KFGVWQEKNMMGRKYMGIVRTTFLISSEGKILKIYDNVKVKDHVSQIIKDIKELKK"
     gene            9613..11754
                     /locus_tag="PLF66_11300"
     CDS             9613..11754
                     /locus_tag="PLF66_11300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015677793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HMZ64674.1"
                     /translation="MANQRTVYRSCTLCEAMCGLRIEIEDEKIIAIRGDKEDAFSRGH
                     LCAKGPELKNIYESPDRIKKPLKRFGDTWKEISWIEALSETAVKIHEIQTKYGKDSVG
                     YYLGNPNVHNYGSILFNPRLAGKINTRNVFVATSMDQLPHHFASFFMFGHQFLLPIPD
                     IDRSNYFLIIGGNPFASNGSIMTAPDVKKRLKAIEERGGKFVVIDPRKTETASNASEH
                     HFIRPGTDVFLLLAMLNLIFSKNLVKNSLALEISDGLDDIKNIAKDFTPSSVSSVTGI
                     SKDSIERITLEFTGAEGATCYGRMGVSTQPFGALCLWLIYVINIVTGNFDKPGGMMFT
                     KPAVDMIGPDNNEAGKGSFDRYRSRVRNLPEFSGEFPVATMADEILTEGDGQIKMLIT
                     SAGNPVLSSPNGKKLENALDKLDFMVSIDFYLNETTSKANIILPPTSGLEHDHYDLIF
                     NIFAVRNVTKYAEPVFPHEEGMLHDWEIFSDLAKRLELVKAGKPLPETIIESKIKPTD
                     FIDRSLQTGPYGKSHGLSLQKLKENPHGIDLGALVPMLPERLRTKDKRIKLCPEIMLT
                     DMNRVKKVLADFKNSNGSNKFMLIGRRHLRNNNSWMHNMPKLMTGPNRCTAIINFKDA
                     ERLGIQNESLVKIKSRVGEVIVSAEVSDEIMEGVVSLPHGFGHTRKGTKLSISKEHAG
                     VSINDITDETEIDALSGNAAFSGTPVEILPV"
     gene            11751..12707
                     /locus_tag="PLF66_11305"
     CDS             11751..12707
                     /locus_tag="PLF66_11305"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0061798 - GTP 3',8'-cyclase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HMZ64675.1"
                     /translation="MREDHLGRKFKKLRISLTSICNFACVYCVSDEEARSNQKVHTCK
                     PLSAEEFAILISKLHTLLDLEEIRLTGGEPTIYPDLIKLILYIKEIGIPKVSITTNGY
                     RLTTIAPELKKVKLDSLNISLDAINSEVFAKMSRGRKLEKTLSGIKAAYDNKIPFKIN
                     ATIMRNWNETEILPLLDFSKKMGVTIRYLELMQMGHLSGNQSDTIFTEKEILQCISQK
                     FSYQKLHREKSSTANYYITEDGYRFGIIANESTPFCSDCNRLRLDSQGSLYGCLSSNH
                     AFSIREAVNSNIPLEPILYQALLKKQNYKFTGSPISMMAIGG"
     gene            12710..12952
                     /locus_tag="PLF66_11310"
     CDS             12710..12952
                     /locus_tag="PLF66_11310"
                     /inference="COORDINATES: protein motif:HMM:NF014636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HMZ64676.1"
                     /translation="MLIRIKTFAVLKNYFKEDFEINLEDHSSIADAIQHLERLNPNSV
                     NILSHCLTAIEEEMADKFTKLKAGQILYLLPPVSGG"
     gene            12954..13481
                     /locus_tag="PLF66_11315"
     CDS             12954..13481
                     /locus_tag="PLF66_11315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010572878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HMZ64677.1"
                     /translation="MEYPHLTRKPIDLSKIFKDSHNPRAGAIVLFSGEVRNHHQGKDV
                     GFLEYEAYESMANKMIDRVLNEAKEKWELNLVYCIHRLGKLEISETAVVVLTAAPHRK
                     EAFEANKWIIDKIKHEVPIWKKETFIDGTSDWVVQCEGCTFASEIQHKNTHAEPTLPP
                     RYSAHPAFLIHGDQQ"
     gene            13468..14064
                     /locus_tag="PLF66_11320"
     CDS             13468..14064
                     /locus_tag="PLF66_11320"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HMZ64678.1"
                     /translation="MINSRLIGVILCGGESKRMGSDKGLLTTEDKYWFIKNFELLDTF
                     FSKTVISIRKSQLETYASKNSNIEYILDSNQSIEGPLKGILSVHEKYPDHDLFVLACD
                     MIQIKSEIIQDLIAKHESNTGFDFYLYKNQNQLETLCGIYTKEGLQKIQSKLETEPKP
                     SFSIYKLIENLRPFLLPISEENQSYFENFNHSPYKNAM"
     gene            14141..15262
                     /locus_tag="PLF66_11325"
     CDS             14141..15262
                     /locus_tag="PLF66_11325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004787889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HMZ64679.1"
                     /translation="MKNTRGLSEQIKTDLETKMVFLGGPRQVGKTTLAKSFIKSEKQY
                     LNWDFLEDRKIIKDHKIDPNLKLIVLDEVHKYVRWRTLVKGFYDKYKESLSILVTGSA
                     RLDHFRKGGDSLQGRYHYLRLHPFSLYEVDPKLKRSTTLDLLRYGGFPEPFIKKDDTF
                     YRRWQKERIAKVIYEDLRDLENVKELSLLELLVDALPSKVGSVLSMNALREDLEISPN
                     TVAHWIQILDTIYYCYRIPPFGAPKIRAVKKSNKLYLWDWAEVENQGARFENMVASHL
                     LKFCHYQEDSQGYKMELRFIRDTDLREIDFVVIRNRKPLFAVECKTGENNISPHIKYF
                     RDRTDIPIFYQIHLGSKEFIDKNIHVLPFESFCKKEKMV"
     gene            15310..16242
                     /gene="moaCB"
                     /locus_tag="PLF66_11330"
     CDS             15310..16242
                     /gene="moaCB"
                     /locus_tag="PLF66_11330"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020776574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional molybdenum cofactor biosynthesis
                     protein MoaC/MoaB"
                     /protein_id="HMZ64680.1"
                     /translation="MKDITSKQISLRTASAEGFVLCSPETIHIIRNNALPKGDLFNIA
                     KAAAMLAAKNTSNLIPHCHPVAIEAMDIEFDTLENVGKDEFAAVKISISAKSIGRTGI
                     EMEVLTAVSIAALTIYDLLKPVDKNILISDIRLTEKKGGKTDKKNKVKPGIKAAILVC
                     SDSTAEGKREDKSGKIIHEMLSHYKVEIADYKIIPDEPTSIQKYVLEWVQSGVDFIFT
                     TGGTGIDPRDKTIDALVEIFDREIDGISETMRAYGQARTPFAMLSRSIAGVIGKSIVI
                     AVPGSSNGARESLEAILPGVFHGRDMLLGGGHPG"
     gene            16370..17479
                     /gene="gcvT"
                     /locus_tag="PLF66_11335"
     CDS             16370..17479
                     /gene="gcvT"
                     /locus_tag="PLF66_11335"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011672071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HMZ64681.1"
                     /translation="MENLKATPLNEIHRNLGARMVPFGGWDMPVQYSSIIQEHLSTRS
                     NAGLFDVSHMGEIFLTGKKADLLSFLEKLTCNKIETMQDFQVQYNAVLNENGGLVDDI
                     TIYKFNDEKYMICSNASNYEAVYEHLLKYNTNLKITNESSNWHQIAIQGPKADEIFSK
                     YTGLNLQDIGYYKFKVLSIKGEEMIVSRTGYTGEDGFEIYSSIKAGVELWSDLLEFGK
                     PFGLVPVGLGARDTLRLEAKYALYGHELNDSRTPVESGIGWIVKEKPTPYFAYDKIIS
                     QKKNGSEYGITGIKLAESGVLREKYSVYDTNNNLIGQTTSGSYSPSLKEGIGLVYIKS
                     DFIKNDTIVYIEIRGSKKKGIIQTESFIKGSVRKK"
     gene            17491..17880
                     /gene="gcvH"
                     /locus_tag="PLF66_11340"
     CDS             17491..17880
                     /gene="gcvH"
                     /locus_tag="PLF66_11340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004781239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HMZ64682.1"
                     /translation="MADTKILDGYKYTEKHEWVKIEGDTAIFGISDFAQNALGDIVFV
                     DLPKIGKTVKKGQSFGTIESVKAAEDLYAPISGEVIEVNPAISKDPALVNKDAFNAWM
                     IKVKNINSSEADSLMDSSKYKEFTASL"
     gene            17899..19686
                     /locus_tag="PLF66_11345"
     CDS             17899..19686
                     /locus_tag="PLF66_11345"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HMZ64683.1"
                     /translation="MVKCFFCGTENSETDKFCRQCGNSLQTALTGAKPDADIELLSSH
                     LSPKFTIEKKIGKGGMATVYLGEQTALQRKIVVKFLNKDISDDEEIRERFILEARTPA
                     RLKHPNIVEVIDVGLCDDRPYYIMEYASGGSVADKQKKYRDQGMMFPLKEAAELIMKV
                     LDALHYCHLNNLQSHRDIKPANIMYRANGEPIIVDFGIAKITDSSITRTRMTMGTANY
                     MSPEQCQGRKDIDGRSDVYSVGIMLFELLTGELPFKGDSGLSIMIKQVKEKLPSLSGK
                     IKNKSERVDPDFSILGPELEAIIEKACAKSRKKRYQSAKEFAEALAKLVGSDIPNTLK
                     QTPIHRSLNWGLIVAMLLLGLGIGGFLGYRMIIVNPKIEVEKNIFIDTDPPGAKVINT
                     TTNIEEGKTPFSIVKEQPGIYNYKLTLDGYKDGITEIQLSNVQKKEVRAIKLDPEVPI
                     DTPDENTNIPDEDSKTAKDAKDAKNTTDSKSTKDAKEATDSKEIKDNGLLSYGGLLWQ
                     TSDIKLMNWYSAISYCRNLKMRLPSRNEMRLPYKSGIRKLMSPCCEYWTATPHEDDPE
                     SAYNISVKSYDTFFSPKLNKFYVRCVARP"
     gene            complement(19688..20707)
                     /locus_tag="PLF66_11350"
     CDS             complement(19688..20707)
                     /locus_tag="PLF66_11350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HMZ64684.1"
                     /translation="MKFHKYTEIPEVEEIGTNLYKIILPQPFYAPNNIYILNGEEPAL
                     IDSGFILNLGLLQRAMKRIGLSFSNIRHIFYTHNHLDHISASLTLRSYTKARHYGMQG
                     MAKFVGDFRRHNEAFQRTMNRLLYKAIRDPEKRKKEIQLSEKGFKELLAALKGSKKTD
                     PILKMDVELVEGDVIDIGDRLIGFMHTPGHNMWHLSPYILGEGIYFTGDLVLQNISSI
                     YAEFDGNLSHYHKSLERLLNLPIQRLLPAHGQEPDDPKRAIKVLIKTLNYHERGVIRR
                     LKLGSYDLHELAVEAMGEKISGSPYYVVALAIIQSIILKLISQNHVRILEIDPPYEKY
                     EWIGE"
     gene            20951..22039
                     /locus_tag="PLF66_11355"
     CDS             20951..22039
                     /locus_tag="PLF66_11355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002971616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HMZ64685.1"
                     /translation="MGNIAEKLSPKNQSIEFNKPTLSKEELETVLDCLVDDMISSGLI
                     KDRFEKDFKSTFKLRNVIAVNSVTAAYHLALISLGISDNDPVLLSTYAPHAALDAILM
                     LRAKPIIVDLGKSSFHMDVEEFNHRVEEHLPKAILIDHTFGCLFDIKKYNTKDIPIIE
                     DYSEAIGADSEDISVGKQGLISICGMQPNHVITTGNGAVVITPNDSLAETIRSQKVGH
                     NEKREPNKPKYDYNLLDFQAAIGIEQISKLGVIIERKKKIAQIYLQSVLAASHETYFK
                     NASQDQFNRFPVVIAKPYEEVERYFKSLQIGTERTISLPLHRILSASNSDFPNAERLY
                     QRGHCIPIYPNLTKDNVNRIANSIKGLY"
     gene            complement(22040..23239)
                     /locus_tag="PLF66_11360"
     CDS             complement(22040..23239)
                     /locus_tag="PLF66_11360"
                     /inference="COORDINATES: protein motif:HMM:NF014298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HMZ64686.1"
                     /translation="MGNLYRLNSLFEIEIKQFPGPIDFDKSMLVRSNIFDYLFLALSS
                     SEDYSLLGEKPSEELLDYWQKNKLETGKPIQIEEPYYDHELFLSKNLLKKNVLVEWGN
                     TSSVNKDGSGIKRKGKTILLSRELNSKYTQLLWKEKLKLNRLNSVICTSALEVDYAVS
                     TMKFPIVLKTEFGFSGRGNFIIKDKKELKENLYRIKVAIRENANRIIVEEWVGDKRMH
                     DFSGLFDINKRSVEFVALTHMFIDQFGSYKGTVIRKEEDRKVREEVYNELAQYLGFFG
                     KCNGPISMDGFTFKKDGLIETQYMSEINFRWSMGRILFELDKKIGKSENDRALVFVSI
                     KAKELKFKTVLSELNKLIKENKNQILLLTPIQNENGKLNPFACFYLSGEYKKLINSQE
                     QLKKLLA"
     gene            complement(23218..24066)
                     /locus_tag="PLF66_11365"
     CDS             complement(23218..24066)
                     /locus_tag="PLF66_11365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016760421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF455 family protein"
                     /protein_id="HMZ64687.1"
                     /translation="MNTINDYCKMILFSGRLSDKLISPPENLEDTRDFNLDIPMTPTR
                     DKSIQISEQKSKIPRLEHLHMSVNRGLAMHHFANHELMATEIFAWAILKFQDLSTEAR
                     KTLLRSLQDEQKHLKLYIDRMTDLGIEFGDKPLNGIFWKYIPMMQTFEKFTAIMSISF
                     EGANLDYATIYQKTFSQFGDDKSAQIMETVFLDELKHVRRGLNVIRSSKDQSISDWDY
                     YISLLEKPFTPRRAKGYFYLPDTRRKVGFTEEFIEKLAEYRDEFSNRKKEIIPEELKT
                     WGIYTG"
     gene            24792..25442
                     /locus_tag="PLF66_11370"
     CDS             24792..25442
                     /locus_tag="PLF66_11370"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pathogenesis-related family 1 protein"
                     /protein_id="HMZ64688.1"
                     /translation="MKSLLLVFLVFLNFNCMQNIMECPDDTLLLKNECSATKDRKAYS
                     KNMALIGLGISTRGSMALAASAAGESSSVSGITIEHNRLRAQENAGLPNLVWDATVAT
                     YALNKVTYLATTNNCVMSHSAGPTNPGYGENLAWASYTSYTGVNAVNAWYNEKSDYNY
                     STNTCASGKVCGHYTQVVWKNSTAIGCAGVVCSNGGGIIFGCNYNPPGNYSGQKPY"
     gene            complement(25654..26535)
                     /locus_tag="PLF66_11375"
     CDS             complement(25654..26535)
                     /locus_tag="PLF66_11375"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HMZ64689.1"
                     /translation="MGESKIMKSIFIKACLVSFLLAQNLYSQKTEISSGVKTYCLPKE
                     GEAVTPSLPPCTNKIAVDIYTPEDKKPIADVLVLNGWSFPRDDWQKKTRILKSAKEKG
                     FRLIFPEMGKTNYESEYFPETKLKWSTTPGGKWVREILIPSLQKNGILVKGSKNFVMG
                     LSTGGRGAAILGFTKPDIFSAAASISGDFDQATMPKDRLMTGSYGPYEENKVRWETVD
                     NVYRSAKDWKIPVYLGHGQSDKVSPFIQTKNFYLKLKELHPNLKIVFNDPVSAEHDYK
                     YWDSEVDPIFDFFSSIK"
     gene            complement(26514..27878)
                     /locus_tag="PLF66_11380"
     CDS             complement(26514..27878)
                     /locus_tag="PLF66_11380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64690.1"
                     /translation="MLNFKQIIERGLSLRLLWDLFMIMAVIMNLHLIVFDLSYLTLRP
                     YYLSHLPAVTKFYDPFLGIEPHRTTEDYLKLVDEYARILDTPDSPEKEEAIHLFSEKL
                     IEQSFKMIDENPFEKVGLTANLQRIKYEIKKKYEVFNGVSAEKSSSKDAFKWFWTYDP
                     KTVKEHIQFFRDTIAPLVAPNYYRHYGLNGKYVDDFFKLDLPFFVLFLLEFLVQWYLS
                     IRNRVYVAWFLYPMYHWYDVLGLIPLAEFRFFRLFRIINMYIILKNSSLTNVGNDIFT
                     RTFIYYTNIIKEELTDMVTVRILTEMQDEIKSGASVNLVTSAVEARREQLKKLIIENI
                     KKGIANPKANETIRKLLAEALVRSSNTASTLQIIPAVIKETVTKDIGLAIFDSMNEVI
                     ISKLTGEQGEENINLLIDNVLDDVILGSQDSEFNKLSEDMAIEIIENMKKAVSVKKWV
                     KAKL"
     gene            28014..28745
                     /locus_tag="PLF66_11385"
     CDS             28014..28745
                     /locus_tag="PLF66_11385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002099331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMZ64691.1"
                     /translation="MLELININKNYNSSEHSLHVLKNINIKINQGEFVAIMGPSGSGK
                     STLLGIAAGLDRPDTGEVIIDSIQIKGLSEDELARLRAERVGFIFQNFQLIRTLSALE
                     NVCLPLILSSKLSEKEIKEKATNLLEKVSLEHRLSHFPSQLSGGEEQRVAIARSFINN
                     PKILFADEPTGNLDAKNGELVMNMLTSLNKENNSTLIIVTHDPKVASLADRILEMENG
                     SLKERETNLDKPQKTKLKAKKKGKK"
     gene            28742..31279
                     /locus_tag="PLF66_11390"
     CDS             28742..31279
                     /locus_tag="PLF66_11390"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HMZ64692.1"
                     /translation="MKLSFQSKFILREILQRPIYSIQFILAVAIGVGTVTGINSYKQN
                     LYEAIGKESRTIMGGDLMIESPSPYSSEIKKFIKENLPENSKLSYSVNFASMIYTPNK
                     ETSLATVKVLDEKFPLYGEIQTKPVGLFNHLKNNETILEETLSKNLKLEIGSYVFIGN
                     EKFKLKGYVLKEPSSVGTFTSMAPTAIITPNAFKKTGLESRGSRIRYNVLVALPNGID
                     SKNFKEENFKKFINKDLTLYHNTEIGSGSQRFINSTFDYMSLLGLSAFFLGAISILIS
                     TRTRLSEKTKEIAVLKCLGATSYWNISIFIREIILLSIIGTTIGIGLGYFIQFFIPDL
                     TGSDFLRGIDPKINFKSFLWGIFIGVFVPIVIGIESIVKITRLSPLFAIRSEMEEKIK
                     IRFLPDKLLFFETILIYLLFFLLASNETGSFLKGFILSGVILLLPILLTIFYQLFRIL
                     SLQLLKTPIFGNTFRLVLKKINQTGSGLALPIIGIGSALTILLLSIIIKNSLLSLSGI
                     NQLDRRPNVFAIDLRKEQIEELKSIQSRFPSKQVLISPIIGARLAGINNKPILKEDTE
                     KDAVKRDWRSTAKTREYFLSYRNEMYETEKVVEGKFWENKGEDEISVEYEFAKSLGVK
                     IGDTLQFNVQGIEVSGKITNTRSVNWADMKPNFVVLFSPGSLESAPAYFITSFLLESP
                     EDRYQFQKELVSKAPNATVIDVEKAIQNFTVILDKVASVIGLMTSFIITSSILLLIAS
                     LYSSKRQREEETSLLRVIGATSRFVFRMYFIEALFVSIYAFLCSLILSLIANYILSTY
                     FLNLRYTIPIWEIVISLCFTILLILSIYLFNLMGLFKKPAKEFLKSV"
     gene            complement(31755..34676)
                     /locus_tag="PLF66_11395"
     CDS             complement(31755..34676)
                     /locus_tag="PLF66_11395"
                     /inference="COORDINATES: protein motif:HMM:NF016358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF499 domain-containing protein"
                     /protein_id="HMZ64693.1"
                     /translation="MNLKPWHTIISPREDLREGRPLDASEFAVHLDHVRDGVAGKDYT
                     DPVKFFQRTYLTESLLDFASQAVRRWNGITVETSPIFNLSTQFGGGKTHSLTLLYHLA
                     KGGKDYQEWNGVNQILGKAGVTSIPEVKTAIFVGTEFSSVSGRTKADEPLRLTPWGEI
                     AFQLGGREGYSLLEKHDTQFISPAGDDLQKLFDPNTSYLILLDEILNYISKHRDYKNL
                     GTQFYHFIQTLSEFVRSRNNIVLAVSIPASELELTPDDIADFDRIKKLLDRLGKAMFL
                     SSGKETNEIIRRRLFEWNGLPPEANKTIAEYLNWLQNHESHLPGWFSMNQAKEHFEAS
                     YPFHPSVISLFERKWQSLPRFQQTRGVLRLLALWVSRTYSEAYKKNQKFPLIDLGNAP
                     LEDDKFRAAVFEQLGEAKLEAAVTTDIAGKKESHSLRLDEEATENISKKTLHRRTAGI
                     IFFESNGGQVTDKLATVPEIKLAIGEPDLDIGLLDSVLQSLLDGCYYLTASGAKYKFS
                     TQENLIKRFVDRNASIDPIEITELLETEIRSVFPNYNGIDLIYFPSQNHQIPDRALLQ
                     FIVLHPSKNMQGVDTLSFVQDSFLKYGNTSRIFKSGLFFCIPESDSNLNQAARKALAW
                     TAIESEASELKIDSEQIKAIKENIQRAKKEIKESVWKSYRFLYYLDKDNKPHKLDLGQ
                     IHSSQSKSLIDLYIQRLKSDGEISDEINPNYLIRNWPPAFTAWGLKSMRDLFYSSPRF
                     PRVLNSENLKNTVIKGVANSLFAIGIRKADLISDIRIGKTIYNSDLDISDEFIILLPE
                     EAKRLQDPPKLTKLEIILPSTNLKVGQSYHLTTRGFDQYSQVYTPSEQKWTTDFGTIT
                     NEGVLIVTEPTIDPIVVSVNAGGLTSQKTIFIEHEKLKHTPIESQGLSKVSTLSWKGI
                     IPLAKWMLFYSKILIKLTQKPKLNIQVSISCEIEKENEEQIKSEIRPLLIELGLEDTL
                     A"
     gene            complement(34700..35035)
                     /locus_tag="PLF66_11400"
     CDS             complement(34700..35035)
                     /locus_tag="PLF66_11400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotyrosine protein phosphatase"
                     /protein_id="HMZ64694.1"
                     /translation="MINRNILFICSGNRDRSPTAEKIYKDDESLSVRSAGTSKDAVRE
                     VTIEDVQWADIIFVMEKKHEERMITKFTRAIKYKEMHVLDIHNDYKFMDPELVKLLQT
                     KVNAILSIN"
     gene            complement(35039..35992)
                     /locus_tag="PLF66_11405"
     CDS             complement(35039..35992)
                     /locus_tag="PLF66_11405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64695.1"
                     /translation="MSERVLNVFLDISIDDLTKQNKEALNSFIHTIGDIDSKENINIY
                     RIRDLGYVPDDDFHEEYLFNKVRIINSLIKKMEVWESEDKLESENPKYYKWEFAYATK
                     ISPFKNILGYMAYLFFHFDQYSFLVTLKNYHGGNIYTIIDYEKLTTSIESYWYQIAFS
                     NPFTNFDKSFYSWYSNLVQPRKYDHNYKHDLYPIDRLNPTNHKKELDRLLIIYLGKYQ
                     KSMSERRGNQQKFESPLFCSEDKAIRLLQSAIGDPNRELFNYDSETKLFIEYKTHPNT
                     PNLYHGYHRLDRDIFLSEVESNHSLDTRRQLEQREKLGNTE"
     gene            complement(35989..37191)
                     /locus_tag="PLF66_11410"
     CDS             complement(35989..37191)
                     /locus_tag="PLF66_11410"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3696 domain-containing protein"
                     /protein_id="HMZ64696.1"
                     /translation="MINNLTIQNFKAFELQSFSLKNLNIFTGINGVGKSSALQSLLLL
                     RQSFLKNKLESLVLNHEDYVSIGTGKDALYSGVLNEFAPIGFYFNDSKEKNQFLFDYL
                     RESDKEELEKKQIERMGTLRLNSSSEFNPSFFDQPLFKSDNLFQYLPAERIGPEDENE
                     MSSEYVIERKELGPKGKFSLDYFESYKTTQLDNPYILHPRTLLDVSGSNSLGVQTNLW
                     LNEITPGTKLSTYEIKEIRKIRGAFSFQQNDGYGPEYKTKNVGFGLTYVLPVILALLT
                     TKPGGMVIIENPESHLHPAGQSKMAELIARCAASGVQVFVETHSDHIINGIMVAAYEH
                     YKEKKKENPDKTIGISNEDVAIYYFSREEGRNYSDVERINLTETGRIKKPPRKFFDQF
                     DLDLRRLL"
     gene            complement(37181..38899)
                     /locus_tag="PLF66_11415"
     CDS             complement(37181..38899)
                     /locus_tag="PLF66_11415"
                     /inference="COORDINATES: protein motif:HMM:NF015210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HMZ64697.1"
                     /translation="MGIFGDLPGAPLENTEKFEKEIFEQNLEIFEQADTQRAEYFFDI
                     LSDLLKTFQLDYNDPRIKLSVKKTGQSGFSLHIGNKAVITIKRDYNELVLEILQPAEN
                     HFYKSSPSFRSIEGKNRSNKNAAVNNVSFAFQKLDIEKNKIKEQWYEIVKSLLDKNPK
                     ARRDSHNYYFYNVIINKSKRKEALKAIPTEISDKYKREVENEEEEFEDYVFDPKDINI
                     SIEQRSLDTLISRIEYEEIDMSPDFQRNSNLWSDQMMSRFIESILLRLPLPAFYFDAT
                     DDKYWLVVDGLQRLFTIYKFVLLKQEKERLRLSGLKILKDLNNKTYDELTGEYKRRIK
                     ECQATCNLIKPVTPAAVKYSIFHRINTGGLRLNNQEIRNALNQRMKGQQAEGPKLLKE
                     LAESKEFKEVIQISAKRMQDREVILRFIAFQIIPARDYKGSMRSFLDQAMEELNKRLL
                     NESKLKQELEQRFKSSLERAKFLFGEKAFSRESERGSIKFNRALFDVWGYYLSKLTIE
                     QEKLLYKNKSKIVEEFNSLLETDFEETIASSTTSSANVALRFEEIYAILAKYIPDLNM
                     EWKNDK"
     gene            complement(38941..>40903)
                     /locus_tag="PLF66_11420"
     CDS             complement(38941..>40903)
                     /locus_tag="PLF66_11420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64698.1"
                     /translation="VTTRSNEQGRFYRLPTDADLQSIQKATKELQKRITSHRGEFSLV
                     PQELTPRGGGSGAGRAFSQRNYGMEQFKDLFTSRQLLTLTTLTRLVKEAKVSKADLEL
                     EKAVKTCLGFVLSRMSDGNSSMGSWQSTGEKLGHLFARQAISMVWDFCEANPFCSSSR
                     NLASMLEWIFMVCDNVIVRNKNNTAMQSSAALHPLADQSADCFFTDPPYYDAVPYADL
                     SDFFYVWLKRTVGNYYPELFKEELTNKADECILDEIKGKDKNYFESTMQKAMAEGNRI
                     LSPKGIGVVVFANKTTSGWEAQLQSMLNAGWMITASWPIDTEMGSRLRAMNSAALATS
                     IHLVCRPRQPLEFWDGKGKLDGTKKMDSGLTGDWRDVLRELPKRIRDWMPRMAKEGIV
                     GADAIFACIGPALEIYSQYERVEKPNGDLVNLREYLEKVWEVVSREALHLVFEDADTE
                     GLEPDARVTAIWLWTLAGGSDSESDSSEEDDDEDKPKSAKAGKLKGGFSLDSDTANRI
                     AQGLGAELKSISSVVEVKGETSRLIPVAERANYLFGKEAKEETLSNKKKKTKEQTLFT
                     PAELGKDTDEWKELTVEKTGQTVLDRLHQSMLLFAAGRTQALKRFLSEEAGNDDRFWK
                     LAQSLNALYPKGTDERRWVEGVQTFRRGV"
BASE COUNT        13655 a         7477 c         6535 g        13236 t
ORIGIN      
        1 ccctaggagt aggaacaaat aatccacgaa tataatagag aatacttttt aaaaaaccaa
       61 tcgtatctaa aaatccttgc gtgtaaaaga aaggaattaa gattttatag tattgaatat
      121 gcgactctgg tagatttaag ttttccgatt cgatccgttc tagaatttct tttcgatttt
      181 ttctccaagt ctttcgacgc gcgacacaaa caaccaattc agcagcagtc tttgcagtag
      241 actgggaaag acaagcctct accaacttct tcttcttctc tggacttctt acccaataga
      301 actcccatgg ttgtaagtta aaagaactgg gagcaagcag cgccaaatct aaacattgac
      361 gcataacgtc ttctggaatt gtttcctccg tatagagacg aatacttctg cgagtctcaa
      421 ctaccttttt gaattcagct ggatcaatag gaggaagttc ttctgccata attaagttcc
      481 ttttacatac tttactttga tgagtaaaat attcttagta acaagtggtc ttttctcttt
      541 taaaaagaaa tctgctttgg gagttgccac tttatagctc ggtttctctg caatcacttc
      601 tatcaacaca ttttctttga ggaaatcttg aaattgaggc aagtcatctt ttgatacgac
      661 tactaacctt tccgattcag attctaatac tttgaaaaat ttctctctat caaatatcgg
      721 ttttgtaaac cttgctgcat aaaaagcata ggatcttttc gaaaacgaca ttcgatatgt
      781 aaatatctca tttttatccg gttccatttg ccgaatcacc gctccaaact gattggaagg
      841 ttggtaggaa gtaagttctg gatagatata aagcccaacg attgaaaaaa ataaacttaa
      901 gcttaaaaca gatacgataa acgaaactgg tagtattttt ttaaaataca gtaaagctac
      961 tagaaagact aagattggaa ttccataaag tatatttaca ttcaatacaa ctagtggaat
     1021 tacaataatg gcaatgatta ataaagctgg aattacaaat aataagttct gaaatacact
     1081 tctactcaga ttcgtttcct tttttatatt taagaattct tcaatatacc tagaaatgaa
     1141 gattgcaccg gcaggaagag cccaataaat atattgaggg agttggtatc tagaaaaact
     1201 tatcaaaaat aaaaatacaa aaagccaaat ataaattaca taataagatt ctttttcttt
     1261 gttttcctct atgctattta atattcttct gaacaatttt acatcagatt tattctgttt
     1321 aatattttta aaaaataaaa acattgtata gaaaatggga agtatgaaac cagcaaatgc
     1381 ccacgaataa tttgtaacaa aataaagtgg atcaaacttt tgattataca tttgtcgata
     1441 aaatcttcca aaagattgta tccataagaa aaaaacagga ccatgactag aaaaatcctg
     1501 atacaagaga taagaccaaa aagcaggaag agaggctact atcaatatgc cacttaaaac
     1561 tttcattcta agcaataact tccaatctct tctgactaat atatccccac caattgcaag
     1621 agctggtatt accatcgaga taggaccttt tgtaataaaa cccatactga taaataaata
     1681 cattaaatag aaccaatttg aattttgttt aactccaagg taataggcat gaaatgcaaa
     1741 agtcaaatag gcgactaaat acacatcgat ttttggatca atgagcattg caaatacacc
     1801 tggagcaaca gaataagcaa ggcttgctaa aattcctctt tctctacttc ctgaaattaa
     1861 agtcgttatt cgaaagatag aatagaaaga taagagtgtt attaaaaatg caggtagtct
     1921 ataggcgaaa ttagaaatac caaatatttt ataagaactt gcaattgtcc aaaaagttaa
     1981 aatcggttta tctagataat ttttaccatt gtcttttaag tgaaaaaact cacctgtttc
     2041 tatcatttct ctggcaattt cggcatattg agaagagtcg atatcaatga cgtcatgtgt
     2101 aagggtaaga acatagggaa taagtgatat taaaaataaa attaaatatt ttgaaaatga
     2161 catcatatct cctttactca tcaagaccgg attgtttcat acattccgcc ataccattgc
     2221 aagaatttga ttctgcatcg tagcatgata aaatctgaga ttgaaacctt gtgcacccat
     2281 caatacactg aatcgtagct tgctttaaaa gctctccttc tattttcatt ttctgtgctt
     2341 tctctgtgca actcacaaaa aaaccacatg cttgtgtgca tttttcttca acggggtctt
     2401 tacaattcgt aaagaaaagg aatacgattg tgaaaaaaag cggaataaaa aattgaatcc
     2461 tgttttgcat tctaaaatcc taatctagaa aatactacta ccattgattc ttcctagcat
     2521 tagaataaca atccaaatta cagaaacttt ttcaatggac tctaagcata tagaaatatt
     2581 cgtatggtta cctcgactcc gctcggtaac catttattaa ctctgctctg catcctcatt
     2641 caatctagtg tgaaaaacca accatttgtt attgtgttta gagcaataca agcatcgaga
     2701 aatcatttca attcaattcg caaagagcct acctttcctg gatcatttta tatttacaaa
     2761 aggtaaacta ttattcccaa gcatatactg tggaagtttt ccatcccatt tgtcaattgc
     2821 attgagttgg atcatgagtg gagtcacaga tccattttta agtcgaagcg tttctgcttc
     2881 tgccttcgca gttgcgattt gttgttctgc ctctattttt attctctgta aatctctttc
     2941 tgctttgagt gctaactgtt ctgcttcttg ctttgactcg attgcttttg cgaaggtcgc
     3001 agaaaattga aaatctttga tagaaaaatc atctatcact atatgatatt tttctaatct
     3061 agattccaat gcagatttta tattatgact tacttgttcg cgtagtgtaa tcaattctgc
     3121 agcagtatac ttagcagtaa cacctttcaa aacttcttgg atagctggat caataatcac
     3181 attttcataa ttgagtccaa tggttctata caaatcattt accatgtctg gttttaaatg
     3241 atagtttact gcgatgacag tagaaatatt ttgtaagtct ttagatgctg cctctgcctt
     3301 tgtttcttct tttaaaacgc gagtatctat ttcaattaca gactgcacaa gtggcatacg
     3361 aaagtgaaca ccttcatcca aaaccttttc tgatacagct cccaaattta atacaacacc
     3421 tttatgtcca ggactaacga tcacacaagg atttacgatc aacaacaata cgatcgctgc
     3481 aacgcctaaa ccaatttttg tatagagatt catccctcca aattgattta tattaataac
     3541 tttttcagcc atagaactct cctttattta aaatatatat tgcagttaac gattcgtaaa
     3601 caattattca gaaaaaaaat attgtagtaa ttttctaatt tgaggaagag tcatatcatt
     3661 tcctaaaaat tcgccatcga tccaaatttc aaaatgcaaa tgaatcgcat cattgttatt
     3721 tcgagcttct cccaacagtc cagaatttcc cgcataacca atcgtatccc ctttccgaac
     3781 ttttgcacca actgtaatcc cgctttgaat agcagataaa tggttgtaag tagtaataat
     3841 tccgcctttg tgctcaatcc aaacctgtct tcctccaaaa tctctatcta catatgtaac
     3901 aggatgagtt tgattgtaag cagatatttc tttgaactca gactcagtca tcggtttata
     3961 attcaaatca gctcgaatca ctactccatc attcgcagaa ataatctttg tatctcttga
     4021 tacatttgta ttcacttgga aaaaatttaa ctttgaacta atatcaagac ctttatgcac
     4081 cccatttcta tactttctag gagcaccagg aagagaataa tcatctgtag gcattacccc
     4141 attttcaatt ggaaatgcaa atccagtcag acgagaatca ataccagtta gatttgtttc
     4201 accaagcagc aaacgaagat tagccagtga aatggaagaa tcagaagttt tattaaaaag
     4261 aattcgaact gtagaagcat caattcctaa aagtgtatct ctgttttttt tatcaggttt
     4321 attattcgta taaatcgtag tccagacttc tcgattcaac acttggattt cgtatgacgt
     4381 ctgagtcata aaagcaaatt cagtctcaac gccagttaaa agtctcttac ttccaaaatc
     4441 gattataacc cattcatttt ctaacttttt atctgtaacc caaaacgtat ttgaattgga
     4501 atcaaaaagg ttttgcccac aatttgaatc agagatacaa gaggatgtgg aaaatttaaa
     4561 tggagcattg gcaagcctac caaaattaat ttctcctttt tgttttaaaa ctacacttcc
     4621 actacttcca aattcaggtc gcagtggtaa gttacaaaaa gtaattaaag ctaaaaaaaa
     4681 agaaacgagg tgtaatcgag aaaaggttaa cttctttagt ataaaagtca gcaaaggcaa
     4741 atatttatga aacatgtata taaattacga ttgaaacttt tctaaagaat taaattattt
     4801 attaccagac ttttgtaaaa tttctaaagc tttggctttt aaaataggat gcactttaaa
     4861 gtcgtcaggc gaaagaggct tgtcatttgt ctgaggagct tctttgattt taaatttatc
     4921 cataccaatc cattgaccaa gagcataaag cattctctga aagatttcat caatcttctg
     4981 ggtatgacct gcttttttat aatagccata tgctaatatt ggaagctttt tttcatacat
     5041 ataataatca ccaagacgaa tgagcccatc tttatagtca cactttagat aaagctctct
     5101 cgcttttgga taatctccgt cattaaaggc tgcgttcccc tctctgatta aggctgctct
     5161 ttcttttgaa tccatagtta gctccaatat tattaatgtc ggaagattat gtccatcact
     5221 aagttaaaat tcactttgga agagagagaa ttttttcttt aaagaagcct acagttgatc
     5281 cataagtctg tctttcatcc tttgtaaaag gattcaattc aactgtatta ttttcaaaaa
     5341 ccaatataac agtagagccc atctcaaaac gacctagctc tgaaccttta tcgattaaaa
     5401 tatttacatt tgtatactca tgttctttgg gaaatcgaat caacttatta gtaacaattt
     5461 tagtatcgta ggttactcga attttaccca cattggacgc tcccactttt acaattgcga
     5521 tctttccata ttcagtttgt aaaaaagtaa tgagtctttc atttttaggg aataatccgc
     5581 gtatgcctcc aacagcagca tcattcactg gaaataactt tccgggcgcg tagtaatatc
     5641 ctaaaatttt tccgtaaaaa ggcgaatgaa tacgatgata gtcctgcgga gaaagataga
     5701 atgtgataaa cttcccattg ttaaaaaacg gatagtattt ttctgaaccc aacaactctt
     5761 tcacactata atcaaagccc tttgcttgaa tcaaagtgtc atcattaata tcaccaaagc
     5821 ttgtaattct agcatcgaca ggcgatacaa ctgcattttc agctgaatca ataatcctcg
     5881 cccctgcttt taaagctcga gtaaaaaatt gatttaatga attgtattct ttaatattta
     5941 attctgcttc acttacattg attttgtaag cctttgcaaa tgcttttaaa attggaacca
     6001 tcataaaacg aggtaatctt aaataagtga aagagccaaa taatttagaa atcaaattct
     6061 taggaagagc agatagtatt aacagataaa tgtctttaaa tatctcataa cgcactccac
     6121 cttctgttaa atatttattc atctcacttc ccaccatttt gacaagtaag atgagggata
     6181 tcaaaatagg tattgctgta aataatgcca ttgtataacc agcattcaat gcgagttcaa
     6241 tgaaataatt tccttctctt aagaataaat aagatgaaga tatcacaact ccgaaaaata
     6301 caattcgaaa taaattcgca aacttttcac ctagaatgta gtatgcactt tgttcccctg
     6361 tataaaacca tccaatgata ctcgcaatac agaagagaaa aaaggaaaaa taaaatataa
     6421 tatagtaggg atcttttccg tgatgaagga ctcgttttaa aaaaaacatc tggtcttcaa
     6481 gaccgacagc atttgcagaa aagagcaaat aaaagatcaa agtggaaaca ataaatcctt
     6541 caaaaaaagc agataacata gagacaagtc cctgcttaaa ggcatggtca gttcgaacgg
     6601 cacccgatat acctgcactc tttcccaaac ccatttctgt agaaagaaaa aagactccac
     6661 ttgactggcc tatataaaca agtatagata ataaactgcc aacaccaatc gcttcgaaat
     6721 taaaggcatt atgaaaaaca actccaataa actgagtaaa tggaatgagt gtttcattaa
     6781 aaattaaaaa atagaatcca aagaaaagaa ttagaccagt cggaacaatt actcctgaaa
     6841 cgcgaccaat gcgtcgaatt ccaccaagaa ctataaatat aacaattgct atgagtgcta
     6901 tcgacaaacc caagcctttt gcatcaaaag cttccttcgc aacataagtc atactcagaa
     6961 ctggaattac tccacccaga ctcaaaaccg ttagtataca accaatagaa aaagcaatcg
     7021 caagccagcg cgctttcaga gctttttcaa taaaatacat tggacctgcc agataacgtc
     7081 cattcggcaa ctttgttcta aatcgaattg caagtgtaga agaaactgcc ctaagtggca
     7141 ttatcaaaaa actaccaacc caaatccaaa agagggctcc tactccaccg atcatcatag
     7201 caagggcaga acctagaatt gccccaggca ataaagaaga agcagtccct gcagaaaaag
     7261 cttgcgagtg aacaatttgt cccttagagc ctttatgatc cattgcacct gataaaattt
     7321 taagtgctaa aaataaaaat cgaacttgag ggaatttcag tttgtaggtg atataaattg
     7381 cggcaaagat aagtaagccg caataagatg caatgatttc atctccaaag aaaataaaat
     7441 tttctttatt catggtttat tctcaatttc ctttgcaaga tcaactaaca atcgaatacc
     7501 gtaaccagta ggtccattga tttgagttcc ggaagcctta cttctccatg cggttccagc
     7561 aatgtccata tgagcccatt tcacatctcc ctctacgaaa ttggctaaga attgtgcggc
     7621 tgaaagtgtg cctgctgctc ttccgcctgt aatatttctg aagtcagcta tatcactctt
     7681 taagtcttct ccgtattctt cccaaagagg caattgccaa actctatctt ccgaactagt
     7741 agaagctgaa tcaatcaagt catataaatg tctagagttt gtcattactc ccgctgcctc
     7801 atgaccaagt gcaataatta ccgcacctgt aagagttgct aaatctacca tatattcggg
     7861 tctatacttt ttacaaacat aagaaataac gtcccctaaa acgagtctac cctctgcatc
     7921 cgtattctga acttcaattg tagttccatt ataagcagta taaacatcac ctggtttagt
     7981 agctgccgca tccggcatat tctcagctac tccaatagca gcaattactc ttactttaat
     8041 tcctagtgct gcaatagctc ctattgcatg tattacggct gctgccccac acatatcata
     8101 tttcatctca tgcatttcag cagatggttt aatgcttatt ccacctgagt caaacgttag
     8161 tcccttccct accagtgcca atgttttctt agttttaaca ttgcttggag aatattccaa
     8221 aactatcatc tttggctttt tgtcagagcc gcggcaaaca gagagtattc ccccaaaatt
     8281 ttctttctct agcctatctt catcaaaaac tgtaattttt aatttatttt cttttgcaat
     8341 ctctttggat ctagaaacaa attcttctgg agtaaaatga tttgcaggta aatgctcgac
     8401 gtatcttgat tcatttaaat acttagatac aattttagat ttttcaagtg catcctttgc
     8461 gaatttctct tcttttacat tttcgattac aaaactaata tcacccactt caattttacg
     8521 atctctaaaa gtctttgaaa gtgaatcgac agggaaggca atgatctcaa gagaatttgc
     8581 aagttggtag actaagttga atgtagaaaa catttcactg agttcttttg aaatatgaat
     8641 ttctaaaccg atatcatacc attttataaa agtatcacct agtttaaaat aataggaagc
     8701 gagttttcga agcgttagtt tactttcctc acccaatcca atataaatga tttgatcatt
     8761 atgattaata aactgttgcc ctgcatctgc ttgaaacatc ttagcatcca tttcaaattg
     8821 aagagctttc ttgatagaat ttggaacctt atccttgaag atgtgctgca ctttatagat
     8881 tgatttggac ttatttggtc caacatacat ttttaatttt gaagaaggta atttcatttt
     8941 ttaagttcct ttatatcttt gattatctga cttacgtgat cttttacttt tacattatcg
     9001 tatattttca aaattttccc ttcagaactg attaagaaag tagtgcgaac aattcccata
     9061 tactttcgac ccatcatatt cttttcttgc caaaccccaa atttctctgc catatctgat
     9121 tcagcatcag acaaaagttc aaagttcaaa tcctgttttt caataaattt cttatgtgat
     9181 ttaagatcat cctttgaaac acccactact gagtagccta attttttaat accttctaga
     9241 ttatctctaa aatcacaagc ctctgtagtg catccgggag tattgtcttt cggatagaaa
     9301 tataaaacga gaccattttt ccccgttata tcacttagct ttactttttt tccctcagca
     9361 ttcaaagcag aaaatgccgg tatttttttc ccaacttcta aatttgtcat aacagtcctt
     9421 tccaagaaca gatttaaaat tctaagtgga acatctagca atttttttta tagaagcgtt
     9481 tctttctcct attcagtata tttcatcttg attcatttag ataaaaattt gaatttgaaa
     9541 aagaatttta atcgcttgca aaaaagaaat taaaatctag aaaattgtaa atacaagaag
     9601 agaggtgaga atatggcaaa tcaacgaacg gtttatagat cctgcacatt atgtgaggca
     9661 atgtgtggat tacgtatcga aatcgaagat gaaaaaatca tagccattcg gggtgataaa
     9721 gaggatgctt tcagtagagg tcatctatgt gcaaaaggtc ctgaattaaa aaatatttat
     9781 gaaagccctg ataggatcaa aaaacctctc aagcgttttg gagacacttg gaaagaaatt
     9841 tcttggattg aagcattaag cgaaacagct gttaaaattc atgagattca aacaaaatat
     9901 ggaaaagatt ctgtaggata ttatttaggc aatccaaatg ttcataatta tggttccata
     9961 ttatttaatc caagactcgc gggaaaaata aatactagaa atgtatttgt cgcaacatca
    10021 atggatcaac taccgcatca ctttgcttct ttttttatgt tcggacatca atttctactt
    10081 cctattcctg acatagatcg atccaattat tttctaatca tcggtggaaa tccatttgca
    10141 tccaatggta gtataatgac agcacccgat gtgaaaaaaa gattaaaagc aatagaagaa
    10201 cgtggcggta aatttgtagt catcgatcca agaaaaacag aaacagcatc taatgcaagt
    10261 gagcatcact ttattcgccc aggaacagat gttttcctac tactcgcaat gctcaatcta
    10321 atcttctcaa aaaatttagt caaaaattct ttggcattgg aaatttcaga tgggttagat
    10381 gatatcaaaa acattgcaaa agattttact ccatcaagtg tttcttctgt aacaggaatt
    10441 tcaaaggatt ctatagagcg aataacgtta gaattcacag gagcagaagg agcgacctgc
    10501 tatggaagaa tgggagtttc tacacaacct ttcggcgctc tttgtctttg gttaatttat
    10561 gttattaaca ttgtaacggg aaattttgac aagccaggcg gaatgatgtt tactaagcca
    10621 gcagttgata tgataggtcc tgataacaat gaagcaggta aaggaagttt tgatcgatac
    10681 agaagtagag ttagaaatct tcctgaattt tcgggagaat ttcctgtcgc tacaatggct
    10741 gatgaaattc ttacagaggg ggatggacaa attaagatgt taatcacctc agcgggtaac
    10801 ccagtattat cctctccaaa tggaaaaaaa ttagaaaacg ctttagacaa attagacttt
    10861 atggtgtcga ttgattttta tctaaatgaa acaacatcaa aggctaatat cattttacct
    10921 ccaacctctg gattagagca tgatcactac gatcttattt ttaatatctt tgctgttcgt
    10981 aatgtcacta agtatgcaga accagtattt ccacatgaag aaggaatgct acacgattgg
    11041 gagatttttt ccgatttagc caaacgccta gaattagtca aagccggtaa acctctacct
    11101 gaaacaatta tagaatctaa aattaaacca acagatttta tagatcgttc tcttcaaact
    11161 ggtccttatg gaaaatcgca tggactgagc ttgcaaaaat taaaagaaaa tccgcatgga
    11221 attgacttag gtgcgttagt tccaatgtta ccggaacgac ttagaacaaa agacaaaaga
    11281 attaaacttt gtccagagat aatgcttact gatatgaatc gagttaaaaa agttttagct
    11341 gattttaaaa atagcaatgg aagtaacaaa tttatgttaa tcggcagaag acatctaaga
    11401 aataataatt cctggatgca taatatgcca aagcttatga cgggtccaaa tagatgcact
    11461 gcaatcatca attttaaaga tgcagaacga ttaggaatcc aaaatgaatc tttagttaaa
    11521 atcaagtcta gagtgggaga agtaatcgta tccgctgagg taagcgatga aattatggag
    11581 ggtgtagtaa gccttccgca tgggtttggt catacaagaa agggcacaaa actatccatt
    11641 tcaaaagaac atgcaggagt tagtataaat gacataacgg atgaaacgga aattgatgca
    11701 ctttcaggaa atgctgcttt tagtgggaca ccagtagaaa ttctacctgt atgagagaag
    11761 accatttagg cagaaagttt aaaaaactga gaatcagcct aacttctatt tgcaacttcg
    11821 cttgcgtcta ttgtgtcagt gatgaggagg ctagatcaaa ccaaaaggtg catacctgca
    11881 aaccattatc cgcggaagag tttgcgattc ttatttctaa acttcacact cttctggact
    11941 tagaagaaat cagactcact ggtggagagc caactatcta tcctgattta ataaaattaa
    12001 tcttatatat aaaagaaatc ggaattccca aagtttcgat cacaaccaat ggatacagac
    12061 tcaccactat cgctcctgaa ttgaaaaaag ttaaattaga ttctttaaat atttctcttg
    12121 atgcaatcaa ttcagaagtg tttgcaaaaa tgagtagagg cagaaaattg gaaaagactt
    12181 tgagtggaat taaagccgct tatgataata aaattccatt taaaattaac gcaaccatta
    12241 tgcgaaattg gaatgaaaca gaaatcctac cacttctaga tttttcaaag aaaatgggtg
    12301 taacaattcg atatttggaa cttatgcaaa tgggtcattt atcaggcaat cagtcagata
    12361 ctattttcac tgaaaaggaa atccttcagt gcatttctca aaaattctca tatcaaaaat
    12421 tacatcgaga aaaatcttca acggcaaatt actatataac cgaagatgga tataggtttg
    12481 gaattatagc aaatgaatca acaccatttt gtagtgattg taatagatta agattggaca
    12541 gtcagggttc tctttatggt tgtttaagtt ctaatcatgc attttcaatt cgagaagcgg
    12601 taaattcaaa tatacctctg gaaccaattt tatatcaggc tctcttaaaa aagcaaaact
    12661 ataaatttac aggcagtcca atcagtatga tggcaatagg aggctaacat tgcttatccg
    12721 tattaaaact ttcgctgtat taaagaatta tttcaaggaa gacttcgaaa ttaacctaga
    12781 agatcattct agcatagccg atgcaataca gcatctagaa agactcaatc caaattccgt
    12841 taatattttg agtcattgct taacggcaat agaggaagaa atggcagata aattcacaaa
    12901 attaaaagca gggcaaattc tatatctcct gccaccagta agtggaggtt aatatggaat
    12961 atccacacct tactagaaaa ccaatcgact taagtaaaat attcaaagat agccacaatc
    13021 cgagagcagg agcaattgtt ttatttagtg gtgaagtcag aaaccatcac caaggaaaag
    13081 atgtaggatt tctggaatac gaagcatatg aatctatggc aaataaaatg attgatagag
    13141 ttctgaacga agcaaaagaa aaatgggaat taaatcttgt atattgcata catcgtctcg
    13201 gtaaactgga aatatcagaa acagcagtcg ttgtattaac ggcagcacct catagaaaag
    13261 aagcctttga agcaaataaa tggattatag ataaaattaa gcatgaagtt cctatttgga
    13321 aaaaagaaac ttttatagac ggaacatcgg actgggttgt gcaatgcgag ggatgcactt
    13381 ttgcaagtga aattcaacat aaaaacacac atgcagaacc tactctacca cctaggtata
    13441 gtgcacatcc tgcatttcta attcacggtg atcaacagta gactaattgg cgttatcctc
    13501 tgtgggggtg agtccaagag gatgggtagc gacaaagggc tgttaaccac agaagacaaa
    13561 tactggttta taaaaaactt tgaactttta gatacatttt tttctaaaac tgtaatctcc
    13621 attcgaaaga gccagttaga aacctatgct tctaaaaatt caaatattga gtatatatta
    13681 gattctaacc aatccataga aggtccattg aagggaatac tttccgttca tgaaaaatat
    13741 cccgaccatg atttatttgt attagcctgc gatatgattc aaataaagag tgaaatcatt
    13801 caagatctta ttgcaaaaca tgaatcaaac accggcttcg atttttattt atacaaaaat
    13861 caaaatcagc ttgaaaccct ctgtggaatc tacacaaagg aaggtttaca aaagattcaa
    13921 tcaaaattag aaacagagcc aaagccaagt ttttctattt ataaactcat tgagaattta
    13981 agacctttcc tactcccaat ctcagaagaa aatcaatctt atttcgaaaa ttttaatcac
    14041 tcaccttaca aaaacgcgat gtgagtaaaa taagcgaata tctatataga tagaaatttt
    14101 atagttgact gaaaaatact catgagtatt tttcagttat atgaaaaata cacgtggcct
    14161 atccgaacaa atcaaaaccg atttagaaac caaaatggtt tttttaggag gtcctagaca
    14221 agtcggtaaa acgactcttg caaaatcttt cattaaatct gaaaagcagt atttaaattg
    14281 ggatttttta gaagacagaa aaatcattaa agatcacaaa attgatccta atcttaaatt
    14341 aatcgtttta gatgaagttc ataaatacgt tcgttggaga acattagtaa agggttttta
    14401 cgataagtat aaagaaagtc tttctatttt ggtgactggt tccgcaagac ttgatcattt
    14461 cagaaaagga ggagattctc tgcaaggaag atatcactac ttgcgactac acccatttag
    14521 cctctacgag gtagatccaa aattgaaacg atctaccact ctagatttac ttcgatatgg
    14581 tggatttcca gaacctttca taaaaaaaga cgatacgttc tatagaaggt ggcaaaaaga
    14641 acggattgca aaagtcattt atgaagactt acgagattta gaaaatgtaa aagagctttc
    14701 cctactggaa ttacttgtgg atgcgcttcc ttcaaaagtt ggatcagttt tatctatgaa
    14761 tgctcttcga gaagatttgg aaatctcacc caatacagtt gcacattgga ttcaaatctt
    14821 agatacaatt tactattgct atcgaattcc tccatttgga gcaccaaaga ttcgagcggt
    14881 aaaaaaatca aataaactct atttatggga ttgggctgaa gtagaaaacc aaggtgcgcg
    14941 ctttgaaaat atggtagcaa gtcacctact caaattttgt cattatcagg aagattcgca
    15001 aggttacaaa atggaacttc gttttatcag agatactgat cttagagaaa tagactttgt
    15061 cgttattcga aatagaaaac ctctttttgc tgtagaatgt aagacagggg aaaacaacat
    15121 ctctccccat ataaaatact ttagagacag aacagatatt ccaatctttt atcaaattca
    15181 tctcggaagt aaagaattca ttgataaaaa tattcatgtt ttaccttttg aaagtttttg
    15241 taaaaaagaa aaaatggttt aatttctttt cacttgacaa cctattttct attttccttt
    15301 gattgaaaca tgaaagatat tacttcgaaa caaataagtc tacgcacagc aagtgcggaa
    15361 ggatttgttc tttgcagtcc agagacaatc catattatac gcaacaatgc actccccaaa
    15421 ggtgatttat ttaatattgc aaaagcagct gcaatgcttg ccgctaaaaa tacttctaac
    15481 ttaattcctc attgtcatcc tgtagcaatc gaggcaatgg atattgaatt tgatacatta
    15541 gaaaatgtag gtaaagatga atttgccgct gttaaaataa gtatatctgc taaatccatc
    15601 ggacgcactg gtattgaaat ggaagttctt acagcagttt caatcgcagc tcttactatt
    15661 tacgaccttc ttaaaccagt tgataaaaat attttaatca gtgatattcg actcacagaa
    15721 aaaaaaggcg gtaagactga caaaaaaaac aaagtaaaac ctggaatcaa agcagccatt
    15781 ttagtttgtt ctgattctac tgcggaagga aaaagagaag ataaatccgg aaaaatcata
    15841 catgaaatgc tatcgcacta taaagtcgaa attgctgatt acaaaattat tccagatgag
    15901 ccaaccagta ttcagaaata tgttttagaa tgggtccaat ctggagtcga ttttatattt
    15961 acaactggtg gaacaggaat tgacccaaga gataaaacaa ttgatgcgtt agtagaaata
    16021 tttgatagag aaatagatgg aatctccgaa acgatgagag cctatggtca agctagaact
    16081 cctttcgcaa tgttatctag atctattgca ggagtcattg gaaaaagtat agtgatcgcc
    16141 gtacctggta gctctaatgg agctcgtgaa agtctagaag caattcttcc gggtgtcttt
    16201 cacgggcggg acatgttact cggtggcggt catccaggat agctcaaaat attttggtcg
    16261 agcctaacca tattatgttc cataggtaga ataaattcag aaaagggaaa atttcatttt
    16321 cattgtttct agtttctaga tattggtaaa aaagaaaaag aggccaatgg tggaaaatct
    16381 taaagctact ccgttaaacg agattcacag aaatcttggt gcacgtatgg ttccatttgg
    16441 tggatgggat atgcccgtcc aatattcaag tatcatccag gaacatctct ctacccgatc
    16501 taatgcaggt ttattcgacg tatcgcatat gggcgagatt tttcttactg gaaaaaaagc
    16561 agaccttctt tcttttcttg aaaaattaac ctgtaataaa attgaaacaa tgcaagactt
    16621 ccaagttcag tataatgctg tgttaaacga aaatggtggc cttgtagatg acatcacaat
    16681 atacaaattc aatgacgaaa aatatatgat atgctctaac gcttctaatt atgaggcagt
    16741 gtacgaacat ttactcaaat acaatacaaa tctgaaaatc acaaatgaaa gctcaaactg
    16801 gcatcaaata gcaattcagg gtccaaaagc agatgaaatt ttctcaaaat atactgggct
    16861 aaacctacaa gatatcgggt attacaaatt taaagttctc tccattaaag gagaagaaat
    16921 gattgtctca cgaaccggct acacaggtga agatggtttc gaaatctatt cttctatcaa
    16981 agcaggagtg gaactttggt cagatctatt ggaatttgga aaaccattcg gtctagtgcc
    17041 tgttggacta ggagcaagag atactttaag actagaagca aagtatgctc tttatgggca
    17101 tgagttaaac gatagccgaa ctcccgttga atcaggaatt ggttggatcg tcaaagaaaa
    17161 accaactcct tattttgctt atgacaaaat catttcacaa aagaaaaacg gatctgaata
    17221 cggtattacc ggcatcaagt tagctgagtc aggtgtgcta agagaaaaat attcagtata
    17281 tgatacaaat aataatttaa tagggcaaac cacttctgga agttactctc cctctttaaa
    17341 agaaggtatt ggtttagtgt atatcaaaag cgattttatt aaaaatgata cgatagtata
    17401 tatagaaatt cgcggatcaa agaaaaaagg aatcattcaa acagaaagct ttataaaagg
    17461 aagcgttcga aaaaaataat aggagcaatt atggcagaca caaaaatatt agatggatat
    17521 aaatacacag aaaaacatga atgggtcaaa attgaaggag atacagctat tttcggaatc
    17581 tctgattttg cacaaaacgc gttaggagat atagtatttg tagatttgcc aaaaatcgga
    17641 aaaactgtta aaaagggtca aagtttcgga accattgaat ctgttaaggc tgcagaagat
    17701 ttatatgctc caattagtgg agaagtaatt gaagtgaatc cggcaatctc aaaagatcct
    17761 gcgttagtaa acaaggatgc gtttaacgca tggatgataa aagttaagaa cataaattca
    17821 tctgaagcag attcattaat ggattcttca aaatataaag aatttacagc tagcttataa
    17881 gaatcggttt ttgaaatcat ggtaaaatgt tttttttgtg gaactgaaaa ttcagagaca
    17941 gataaattct gtcgtcaatg tggaaatagc ttacaaacag cactcacagg agctaagccg
    18001 gatgcggata ttgaattact cagttcccac ttatcaccaa aatttacgat cgaaaagaaa
    18061 attggtaaag gtggtatggc aacagtttac cttggagaac aaactgcctt acagcgcaaa
    18121 atcgttgtta agtttttaaa taaagatatt tcagatgatg aagaaataag agaacgtttt
    18181 attttagaag caagaacacc tgcaagatta aagcatccta atattgtgga agtaatagat
    18241 gttggactct gtgacgaccg accttattac ataatggaat atgcttctgg cggatctgtt
    18301 gcagacaaac aaaaaaaata tcgtgaccaa ggtatgatgt ttcccttaaa ggaagcagca
    18361 gaactgatca tgaaagtttt agatgcgtta cactactgtc atttaaataa cttacaatct
    18421 catcgcgata tcaaacccgc aaatatcatg tatagagcaa acggagagcc aatcattgtt
    18481 gattttggaa tcgcaaagat tacagactct tccatcaccc gcactcgtat gacgatggga
    18541 acagcaaact acatgagtcc tgagcaatgc caaggcagaa aagacattga tggtcgtagt
    18601 gatgtatatt ctgtcggaat tatgctcttc gaattgttaa ctggagagct tcctttcaaa
    18661 ggagatagtg ggctttccat catgatcaaa caggtaaagg aaaagttacc aagtctttcg
    18721 ggaaaaatta aaaataaatc agaaagagtg gatccagact tttcaatact tggtccagaa
    18781 ttagaagcga taatcgaaaa ggcatgtgca aaatctagaa agaaaagata tcaatctgca
    18841 aaagaatttg cggaagcact tgctaaacta gttggttcgg atattccaaa taccctgaag
    18901 caaacaccga ttcaccgcag tctgaattgg ggcttaattg ttgctatgtt actacttggt
    18961 cttgggatcg gcggttttct aggatataga atgatcattg taaatcctaa aattgaagta
    19021 gaaaaaaata tctttattga tacagatcca ccgggtgcaa aagtaatcaa tactacaaca
    19081 aatatagaag aaggaaaaac tccattctca attgtaaaag agcaaccagg aatctataat
    19141 tataagttaa cgttagacgg ctataaagat ggtattaccg aaattcaact gagtaatgtc
    19201 caaaagaaag aagtaagagc tatcaaatta gatccagaag ttccgattga tacaccggat
    19261 gaaaatacaa atatcccgga cgaggattct aaaacagcaa aagatgctaa ggatgcaaaa
    19321 aacacaactg atagcaaatc cacaaaagat gctaaagaag caactgattc aaaagaaata
    19381 aaagacaatg gtctcttatc gtatggtgga cttctctggc aaacttctga tataaagctc
    19441 atgaattggt attcagcgat tagttattgt agaaatctaa aaatgagact tcctagtaga
    19501 aatgaaatgc gactaccgta taaatctgga atccgcaaac taatgtcgcc ttgttgtgaa
    19561 tattggacag ctacaccaca tgaagatgat cctgaatcag cttacaatat ttctgtaaaa
    19621 agttatgata ctttcttttc acctaaatta aataaattct atgtgagatg tgtagcgaga
    19681 ccttaatcta ctcaccgatc cattcatatt tttcataggg tggatctatc tccaaaattc
    19741 gaacatgatt ttgagatatt aattttaaaa taatggattg tataatcgct aaagccacaa
    19801 cataataagg gctgcctgaa attttttctc ccattgcttc aacggctaac tcatgtaagt
    19861 cgtaagagcc aagcttaagc cttcgaatca cacctctctc atgataattt aaagttttaa
    19921 ttaaaacttt gattgctctt tttggatcat ctggttcctg cccatgagca ggcaataatc
    19981 gttgaatcgg taaattaagc agacgttcaa gggatttgtg ataatgactt agatttccat
    20041 caaattcagc atatatagat gaaatatttt gtaagaccaa gtctcctgta aaataaatcc
    20101 cttctcctaa aatataggga cttaaatgcc acatgttatg ccctggtgta tgcataaaac
    20161 caatcaatcg atctccaata tcgatcacat caccttccac caactcaaca tccattttca
    20221 aaattggatc tgttttcttt gagcctttca aagcagcaag caactcttta aaaccctttt
    20281 cagataactg gatttctttt tttctttttt cgggatcccg aatcgccttg tagagcaggc
    20341 ggttcatggt tcgttggaaa gcttcattgt gccttctaaa atcacctaca aactttgcca
    20401 ttccctgcat cccataatgc ctagcttttg tgtaagaacg caaagtgaga cttgcactta
    20461 tatgatctag atgattgtga gtgtaaaaaa tatgtcggat gttcgaaaaa gacagaccga
    20521 tccgcttcat agctcgctgc aataatccta aattcaaaat aaatccggaa tctattaaag
    20581 caggttcttc tccattcaag atatagatat tatttggtgc ataaaaaggt tgtggcagaa
    20641 tgattttgta gagattggtt ccaatttctt ctacttctgg aatttcagtg tatttgtgga
    20701 atttcatctt attcaatgct ttcttccatc tctatttgag caagttgttt ctggggaata
    20761 gaatcaagtt tgagttttta tttataatgc agaatgcgat ttccatttct aaaatacact
    20821 ctgcctaaat agagtctgac tcgtattcct tcgtttgctc tcatagaatt ggaaaatgga
    20881 ttgatttttt tctttacaag ttatgtctca ttacaataat gagacataac taaatataaa
    20941 ggcatcaaca gtgggaaata ttgcagaaaa actatcacca aaaaatcaat caatagaatt
    21001 caacaagcca actctctcta aagaagaatt ggaaactgtt ctcgattgtc tagtcgatga
    21061 tatgatatct tcgggattaa ttaaagatag atttgaaaaa gattttaaat ctacttttaa
    21121 attacgtaat gttatagctg taaacagtgt aactgctgct taccatcttg ctttaatctc
    21181 gctaggcata tcggataacg atccagtatt gctctcaacg tatgctccac acgcagcttt
    21241 agatgcaatt ttaatgctta gagcaaaacc tatcatagtt gatctaggta aatcctcctt
    21301 tcatatggat gtagaagaat tcaaccatcg agtcgaagaa catctaccca aagctatttt
    21361 aattgatcat acatttggtt gcttatttga tataaaaaaa tacaacacga aagatattcc
    21421 aattattgag gactactcag aagcaattgg cgcagattca gaagatattt ctgttggcaa
    21481 acaaggtcta atttctattt gtggaatgca accaaatcat gtaatcacaa ctggaaacgg
    21541 agctgttgtt ataacaccta acgattctct tgccgaaacc attcgttctc aaaaagttgg
    21601 acacaacgag aaaagagaac caaataaacc taaatatgat tacaatttac ttgatttcca
    21661 agcagctatt ggaattgaac aaatttctaa gctaggtgta atcattgaac gaaaaaagaa
    21721 aattgctcaa atctatttac agtcggtttt agccgcatca cacgaaacct attttaaaaa
    21781 tgcaagccag gatcagttta atcgctttcc tgttgtaatc gcaaaacctt acgaagaagt
    21841 agaaagatat ttcaaatccc ttcagattgg aacagaaaga actatctcct tgcctttaca
    21901 tagaatttta agtgcgagta acagcgattt tccgaatgca gaacgtctct atcaaagagg
    21961 acattgcatt cctatctacc caaatcttac aaaggacaat gtaaatagaa ttgcaaattc
    22021 gattaagggg ctttattaat caagctaaga gcttttttaa ttgctcctgt gaattaatta
    22081 actttttata ttcgccactt agataaaaac aagcaaaagg atttaatttt ccattttcgt
    22141 tttgtattgg agttaataga agaatttgat ttttattttc ctttattaac ttatttagtt
    22201 ctgataaaac tgttttaaac tttaattctt ttgctttaat tgaaacaaaa acgagtgcgc
    22261 gatcattttc agattttcca atctttttat ccagctcaaa taaaattcta cccattgacc
    22321 atctaaaatt gatttcactc atgtattgcg tttcaatcaa tccatctttt ttaaaagtaa
    22381 aaccatccat tgaaattggt ccattgcatt tcccaaaaaa acctagatac tgcgctaatt
    22441 cattgtaaac ttcttctctt acttttctat cttcctcttt tcgaataacg gttcctttat
    22501 aagaaccaaa ttgatcgata aacatatgag ttaaggcaac aaattcaaca cttcgtttgt
    22561 ttatatcaaa taacccactg aagtcatgca ttcttttatc accaacccat tcttctacga
    22621 taatcctatt tgcattctct cggattgcaa ctttgatccg atataaattc tcttttaatt
    22681 cttttttatc ttttataata aaatttcctc ttccagagaa tccaaattct gtctttaaaa
    22741 caattggaaa tttcattgtc gaaacagcat aatcaacttc taaagcacta gtgcaaatta
    22801 ctgaattaag tcgatttagt tttaactttt ccttccaaag aagttgtgta tattttgaat
    22861 tcaattctct cgataaaaga atagtctttc cttttctttt gattcccgac ccatctttat
    22921 tgacactgct tgtatttccc cactcaacta aaacattttt ctttaaaaga tttttactca
    22981 aaaagagttc atgatcataa tatggctctt ctatttgaat tggctttcct gtttccaatt
    23041 tattcttttg ccaataatcc aataactctt cggaaggttt ttcacctaac aaagaataat
    23101 cctcactact tgataaagct aaaaaaagat aatcaaatat attactgcga acaagcattg
    23161 atttatcaaa atcaattgga cccggaaact gtttaatttc aatttcgaat aaagaattta
    23221 acctgtatag attccccatg tttttaactc ctctggaata atttcctttt tacggtttga
    23281 gaattcatct ctatattctg ccaatttttc tataaactct tctgtaaaac ccactttcct
    23341 gcgagtatcc ggcaaataaa aatatccctt ggcacgtctc ggcgtaaaag gtttctctaa
    23401 caatgagata taataatccc aatctgaaat tgactgatct tttgaagagc gtataacgtt
    23461 taaacctctt cttacatgtt tcaattcatc taaaaaaact gtttccataa tttgtgcact
    23521 tttatcatca ccaaattgag aaaaagtctt ttggtatatt gttgcataat caagattagc
    23581 cccttcaaag gaaatagaca tgatagctgt aaatttttca aaggtttgca tcattgggat
    23641 atatttccaa aatataccat tgagaggttt atctccaaat tctattccca aatccgtcat
    23701 tctatctata taaagcttta aatgtttttg ctcatcctgt aaacttctta atagcgtttt
    23761 tcttgcttct gtcgaaaggt cttgaaattt taaaatcgcc caagcaaata tctcggtagc
    23821 cataagttca tgattagcga aatgatgcat tgcaagtcct cgattcacac tcatatgcaa
    23881 atgttccaaa cgaggaattt tagatttttg ttctgatatt tgtattgact tatcccttgt
    23941 tggagtcatt gggatatcta aattaaaatc ccttgtatcc tctaaatttt ctggtggaga
    24001 tattaactta tcacttaacc taccggaaaa gagaatcatc ttacagtaat catttattgt
    24061 attcattcaa ttcatcactt tttacaaaat tacgttgtgc gtttactgtt agcctaattt
    24121 gtttttgcct atgaacagat ttatcaactc cactaaaaca gaaacattta attttaccga
    24181 ttccatagtg aatacgagta atttcataaa atatgttaaa tctctgttat gcgaggctgt
    24241 tattaaatat ttttctctat aatttaattt ggggttattc tagcaagaat gtttatattg
    24301 atatttgcta tgcaaaatgc aatttatctt tcttgtctag aatgagaact aacgattcat
    24361 tctaataaat gacccaactt gatcttttta ataatacctt ttttagtgta tatacattga
    24421 ataacggata agtattttta tatacatcta acgtttttat aaaggtataa attaaaatca
    24481 ataaataaaa ttgatttttc acgagcatat agaagaactc cgagcttacc cgatttttta
    24541 ttacaacctc caatagttca acggagatag attccttgaa agattagttc aacaatttat
    24601 atcttcaatg aatgtttttt atttttaaat taagcttaac tacaagtaag aatttttcac
    24661 tagaatttaa aacattgatc tatttaaaat ccaaagagat gggataaatt caatttgttt
    24721 gcaatttgtt ccgaataatt atgattgcat tcaccatact ttgcaaaaac catgcaggaa
    24781 ggatggttca tatgaaatct ttattattag tattcttagt atttttaaat ttcaattgta
    24841 tgcaaaatat tatggaatgc cccgatgata ctcttttact aaaaaatgaa tgtagtgcaa
    24901 caaaagatag aaaagcatat agtaaaaata tggctctcat tggattgggg atttcaacaa
    24961 gaggatcaat ggcattggca gcaagtgcag ctggtgagtc ttcttctgta agtggaatta
    25021 ccattgaaca caacagactc cgtgcacaag aaaatgccgg cttaccgaac ttggtttggg
    25081 atgcaacagt tgctacttat gcactaaata aagtaaccta tctagcaact acaaataatt
    25141 gcgtaatgag tcattcagca ggtcctacaa atccaggcta tggagaaaat ttagcttggg
    25201 ctagttatac aagttacaca ggtgtcaatg cagttaacgc atggtataat gaaaaatctg
    25261 attacaatta ttctacaaat acttgtgcat caggaaaagt ttgtggtcat tacacacaag
    25321 tagtttggaa aaattcaact gcgatcggct gcgcgggcgt cgtctgttcc aatggtggag
    25381 gcattatatt tggatgtaac tataatcctc caggaaatta ttcaggccag aagccatact
    25441 aacgcatcat tgcgcacaat gtgattaatg taagaatttt taaccacatg cgaaggcgat
    25501 ggtcgcaaat acttggggcg aagggcagga taaacacaga tgaacaccga taaaagatac
    25561 aatgattcag aattatctat cgtttctgta tcgtttcctt atcgttgctc tcggtggtaa
    25621 tcttttaagt ttttttgtta gatcgaactc gccttatttt atactagaaa aaaaatcaaa
    25681 aattggatct acttctgaat cccaatactt gtaatcatgc tctgctgata ctggatcatt
    25741 gaatacaatc tttagatttg gatgtagctc ctttaactta agataaaaat tctttgtttg
    25801 aataaaagga gaaaccttat ctgattgtcc atgccccaaa taaactggta ttttccaatc
    25861 tttcgcactg cgatagacgt tatccacggt ctcccatctt actttatttt cttcataagg
    25921 tccatatgac cctgtcatca gtctatcttt tggcatagtc gcctgatcaa agtcccctga
    25981 tatagaggca gctgcagaga aaatatctgg ctttgtaaaa cctaaaatag ctgcccctct
    26041 tccgcctgtc gatagaccca ttacaaaatt tttactacct ttaactaaaa tcccattttt
    26101 ttgtaaggat ggaattaata tctcccttac ccattttcca ccaggagttg ttgaccattt
    26161 taattttgtt tcagggaaat actctgattc ataattcgtt ttacccattt caggaaaaat
    26221 caaacgaaat cctttttcct ttgcactttt caaaattcta gttttctttt gccaatcatc
    26281 tctaggaaaa ctccaaccat ttaataccaa aacatccgca atcggctttt tatcttcagg
    26341 agtataaata tctacagcaa ttttatttgt gcaaggaggc aaagaaggag taaccgcttc
    26401 cccctcttta ggcaaacaat atgttttaac tccactgctt atttctgtct tttgagaata
    26461 caaattctga gccagtaaaa acgagacaag gcatgctttt ataaaaatag atttcataat
    26521 tttgctttca cccatttttt aactgataca gcttttttca tattttcaat aatttcaatc
    26581 gccatatctt cacttaattt attgaattca ctatcttgag atccaagtat cacatcatct
    26641 agaacattat cgattaatag attgatattt tcctcaccct gctctcccgt taatttagaa
    26701 ataatcactt cattcattga gtcaaagata gcaagaccaa tgtccttggt tacagtttct
    26761 tttatgactg ctggaataat ttgcaaagtt gatgcagtgt ttgaagaacg aaccaatgct
    26821 tctgcaagta atttgcgaat ggtttcattt gctttaggat ttgcaattcc ctttttaata
    26881 ttttctatga tcaatttctt taactgttct cttctagctt ccactgcaga agtaactaaa
    26941 ttgacagaag ctcccgattt aatttcgtct tgcatctctg tcaagattcg aaccgttacc
    27001 atgtcggtta actcttcttt aattatgttt gtataataga taaaagttct tgtaaaaata
    27061 tcatttccaa catttgtgag actactattt tttaagatga tatacatatt gattatacgg
    27121 aacaatcgga aaaatctgaa ttcggctaac ggtatcaatc caagaacgtc ataccaatga
    27181 tacattggat ataaaaacca agcaacatac actcgatttc gaattgatag ataccattga
    27241 accaaaaatt ctaagagaaa taatacaaag aagggaagat caagcttaaa gaaatcatct
    27301 acatactttc catttaatcc ataatgcctg taataatttg gagcgactag aggtgctata
    27361 gtatctctga aaaactggat atgctctttt actgtttttg gatcataggt ccaaaaccat
    27421 ttaaatgcat ctttagatga tgatttttct gcactgactc cgttgaatac ttcatatttt
    27481 ttcttgattt catatttaat tctttgtaaa tttgcggtta atccaacctt ttcaaatgga
    27541 ttttcatcaa tcatcttaaa ggactgttca attaactttt cagagaataa atgaatcgct
    27601 tcttcttttt ctggcgaatc tggtgtatct aaaattcttg catattcatc gactaacttt
    27661 aaataatcct cagtcgttct atgtggttcg attcctaaaa atggatcata aaattttgtt
    27721 acggcaggta agtgagataa ataataaggt ctcaaagtaa gataagatag atcaaaaaca
    27781 attaaatgta aattcatgat tactgccata atcataaata aatcccaaag aagtcgcaaa
    27841 gacaaaccac gctcaataat ctgtttgaag tttaacatat tcgatcaaaa taaaactgag
    27901 aatggcattt ggcaagttta attagtagaa aattttttgg aacttatgaa tttggaaaga
    27961 ggtcatatta gaatcactaa cacaaaagta gtatatgaga gatcgaggaa gctttgctag
    28021 aacttatcaa tatcaacaaa aattataatt catcagaaca ctctttacat gttctaaaaa
    28081 atattaacat taaaatcaac caaggtgaat ttgtcgcaat catgggacct tctggctcag
    28141 gaaagtccac cttacttgga attgccgcag gtctagatcg acccgatacg ggagaagtga
    28201 tcattgattc tattcaaata aaaggactca gcgaagatga actggctcga ttgagagcag
    28261 aaagagttgg ctttatcttt caaaactttc aactgattcg cacgttaagc gctctagaaa
    28321 atgtttgcct tccattgatt ttatctagta aattaagtga aaaggaaata aaagaaaaag
    28381 cgacaaatct tctagaaaaa gtttccttag aacatagact ctcccatttt ccttctcaac
    28441 tttccggggg cgaagaacaa agagtagcta tcgcacgatc ctttatcaat aatccgaaaa
    28501 ttttattcgc tgatgaacca accggaaact tagatgctaa aaacggtgag cttgtaatga
    28561 atatgttaac ctcactcaat aaagaaaata attcaacgct tatcatagta acccatgatc
    28621 caaaggtagc atctctagcc gatagaatct tagaaatgga aaatggttct cttaaagaga
    28681 gagaaacgaa tctagataaa ccacaaaaaa cgaaacttaa agcaaaaaag aaaggtaaaa
    28741 aatgaaactc tcctttcaat ctaaattcat tttaagagaa attctacaac gtcccatcta
    28801 ttccattcaa tttatattag ccgttgccat tggagtcgga actgtaaccg gtatcaattc
    28861 ctataaacaa aatctatacg aagctattgg aaaagaatct agaacaatca tgggcggtga
    28921 tttaatgatt gaatctccaa gtccatattc tagtgaaatt aaaaaattta taaaagaaaa
    28981 tctccctgaa aattcaaaac tgtcttattc tgtaaacttt gcatcgatga tttatactcc
    29041 caataaagaa acgtcacttg ctactgtaaa agttctggat gaaaaatttc ctctctatgg
    29101 tgaaattcaa acaaaacctg tcggactttt taatcatcta aaaaataacg aaactatctt
    29161 agaagagact ttatctaaaa atctgaaatt agaaattgga agttatgtat ttatcggaaa
    29221 tgaaaaattc aaactaaaag gttatgtttt aaaggagcca agttctgttg gaacgtttac
    29281 ctcaatggca ccaacggcta tcataacacc taacgctttt aaaaagacag gtttagaatc
    29341 taggggaagc agaattcgtt ataatgtttt agtggctctg ccaaatggaa ttgattcaaa
    29401 aaactttaaa gaagagaatt tcaaaaaatt catcaacaaa gatttaactc tatatcacaa
    29461 tacagaaata ggatcaggct ctcaacgctt tataaacagc acctttgatt atatgagcct
    29521 actcggactc tctgcattct ttttaggagc aatttcaata ttaatatcca ctaggacaag
    29581 actaagtgaa aagacaaagg aaatcgctgt tctaaaatgc ttaggcgcaa cctcttactg
    29641 gaatatttcc atttttatta gagagatcat tcttttatca atcattggaa cgacaatcgg
    29701 aatcggttta ggttatttta tccaattctt tattccagat ctaaccggat ctgatttttt
    29761 aagaggaatt gatcccaaaa ttaatttcaa gtcctttcta tggggaattt ttattggtgt
    29821 attcgttcct attgtaattg gtatagagtc tatcgtaaaa ataacaagac tgagccctct
    29881 ctttgccatt cggtctgaga tggaagaaaa aattaaaatc cgtttcctac ccgataaatt
    29941 gctcttcttc gagacaattc tcatctacct actcttcttt ctattagcaa gcaatgaaac
    30001 agggagtttt ctaaaaggtt ttattttaag tggtgtcatt ctactattac ctatcttact
    30061 cacaattttc tatcagcttt ttagaatctt aagcttacaa cttctaaaaa cgcctatctt
    30121 cggaaacacg tttcgactgg ttctaaaaaa aataaatcaa acaggaagtg gattggcact
    30181 cccaatcatc ggaattggat ctgctcttac aattcttctt ttatccatta tcattaaaaa
    30241 tagtttactt agcttaagtg gaatcaatca attagataga agacctaacg tatttgcgat
    30301 tgatcttcga aaagaacaaa ttgaagaatt gaaatcgatt caaagtcgat tcccatccaa
    30361 acaagtttta atttctccta tcatcggagc aagactcgca ggtataaaca acaaaccaat
    30421 cttaaaagaa gataccgaaa aagatgcagt caaaagagac tggcgatcaa cagctaaaac
    30481 aagagaatat tttttatctt atcgaaatga aatgtatgaa actgaaaaag ttgtggaagg
    30541 aaaattttgg gaaaacaaag gagaagatga aatctctgtc gaatatgaat ttgccaaaag
    30601 tctaggtgtc aaaattggag acacacttca gttcaatgtg caaggaatcg aagtatctgg
    30661 aaaaataaca aatactcgca gtgtaaactg ggcagatatg aaaccaaact tcgttgtatt
    30721 attttcaccc gggagtttag agtcagcccc agcttatttt atcacatcct tccttttaga
    30781 aagtccagaa gatagatatc aattccaaaa agaattagta agtaaagcgc ctaacgctac
    30841 agtaatagac gtagaaaaag caattcaaaa ctttacagtc attctagata aagtcgctag
    30901 tgtaattggt ctcatgactt ctttcatcat tacatcttca attttactac taatcgcaag
    30961 tctttattct tctaaaagac aaagagaaga agaaacctct ctacttcgag tcatcggagc
    31021 cacatctaga tttgtattta gaatgtattt cattgaagct ttatttgtga gcatctacgc
    31081 ctttctatgc tctctcattc tttctctcat tgcaaattac atcttatcta cttattttct
    31141 aaatctaagg tatacaattc caatctggga aatcgttatt agcctctgct tcactatttt
    31201 gctcattctt tctatttatc tattcaactt aatgggtcta ttcaaaaagc cagcgaagga
    31261 gtttttgaaa tcagtgtagg ggtttttatt gcgacttagt cagtcgcgct gatcccttaa
    31321 atacttttaa gatttatttt tttcttattc ccgcttccca gcggacgggg attattcata
    31381 cttttttctt ttctttttgt aaagaaaaga aaaagtatgc aaaaaagaaa agaaacccga
    31441 aactgcgatt aatcgttata acacgcagga ttctccggtt gggctttttg tatttcggtt
    31501 tttttagtga gaaggtaggt tgttagtgtt tgtggatttt ttattttttg tcgattcagt
    31561 gttactatat agaaaacgtt tattttttct tcttacgaaa agattctcat agtggaacgc
    31621 cccctttgga aagggggaat aagggggatt ggaagtataa aaaagtcact ctgcatcaat
    31681 atcaaaatac gaaaaagctt tgtaaatata cttctctttc ttaattcata actaatccga
    31741 aatttaaaga ggctttaagc tagtgtatct tccaaaccca attcaattaa cagaggacga
    31801 atttctgact ttatttgctc ttcattttct ttttcgattt cacaggaaat acttacttga
    31861 atatttaatt ttggtttttg tgttagctta atcaatatct tactataaaa tagcatccat
    31921 ttcgctaaag gaattattcc tttccatgag agagttgaaa ctttgcttaa tccttgactc
    31981 tcaataggtg tatgctttaa cttctcatgt tctatgaaga ttgttttttg tgaagtcaat
    32041 ccacctgcat taacactaac aacaattgga tcgatagttg gttctgtaac aatcaataca
    32101 ccttcattcg taatcgttcc aaagtcagta gtccatttct gttccgaagg agtatagact
    32161 tgcgaatact gatcgaatcc tctagtcgtc agatgatacg attgtcccac ttttaaattt
    32221 gtagaaggca agataatttc taactttgtt aacttaggtg ggtcttgtaa tcgctttgct
    32281 tcttctggta gtagaataat aaactcatcc gaaatatcta aatcagaatt ataaatagtc
    32341 tttcctattc ttatatcaga aattaaatca gcctttctaa ttccaatggc gaaaagacta
    32401 ttagccacac ctttgataac agtatttttt aaattctctg aattgagaac tcttgggaat
    32461 cgaggggaag aataaaataa gtctctcatt gattttaaac cccatgcagt aaaagcagga
    32521 ggccaatttc ggattagata atttggattt atttcatcgc tgatttctcc atctgatttt
    32581 aatctttgga tgtatagatc aattaatgat ttcgattgac tcgaatggat ttgacctaaa
    32641 tctaatttat gtggtttatt gtccttatcc agataatata agaacctata agacttccaa
    32701 acactttctt taatttcttt tttagctctt tgaatatttt ctttgattgc tttgatttgt
    32761 tcggaatcaa ttttcaattc agatgcttca ctttcaatag ccgtccaagc caaagctttg
    32821 cgtgctgctt ggtttagatt cgaatcactt tcagggatac agaaaaacaa accactttta
    32881 aaaatacggc tagtatttcc atatttcaaa aacgaatcct gcacaaaaga tagagtgtca
    32941 acaccttgca tattcttact tggatgtaat actataaatt gaagcaaggc tctgtctgga
    33001 atttgatgat tttgacttgg aaaataaatt aaatctattc cattgtaatt tggaaataca
    33061 gaacgaattt ctgtttcgag taattcagta atctctattg ggtcaatgct tgcatttcta
    33121 tcaacaaatc gtttgattaa attttcttga gtggagaatt tatatttggc tccacttgca
    33181 gtaagataat aacaaccatc tagtaacgat tgcaaaacag aatctaaaag ccctatatcc
    33241 aaatcaggtt cgccaatggc taacttgatt tccggaactg tcgccaactt atccgtaacc
    33301 tgtcccccat tggattcaaa aaaaataatt ccggcagttc ttctatgcaa agttttttta
    33361 gaaatatttt ctgtagcttc ttcatctaag cgtaaggaat gggattcttt ttttcctgca
    33421 atatctgtcg taacagccgc ttctagtttt gcctcaccca attgctcaaa gacagccgct
    33481 ctaaatttat cgtcttctag tggagcattg cctaaatcaa tcaaaggaaa cttttgattt
    33541 ttcttatatg cttctgaata agttctagaa acccaaaggg ctaataggcg taaaactccc
    33601 cgtgtttgtt gaaatcgagg aagggattgc catttgcgtt caaataaaga aatcacagaa
    33661 ggatgaaatg gataggacgc ttcaaaatgc tctttagctt ggttcatgga aaaccatcca
    33721 ggcaagtggc tttcatgatt ttgtaaccag tttaaatatt ctgcgattgt tttatttgcc
    33781 tccggtggaa gaccattcca ttcaaataat cgacgacgga taatttcatt tgtctccttt
    33841 ccagaagaaa gaaacattgc tttaccaagt ctatctagca gttttttaat tctatcaaag
    33901 tcagcaatat cgtcgggcgt taattccagt tcagatgcag gaatagaaac tgcgagaact
    33961 atattatttc tactccttac aaattcagat aaagtttgta taaaatgata gaattgagtc
    34021 cctaaatttt tataatcgcg atgtttacta atataattta aaatttcatc taaaagaatt
    34081 aaataggaag tattcggatc gaataacttt tgcagatcat ccccagcagg agaaataaat
    34141 tgagtatcat gtttttctaa aagagaataa ccttctctcc caccaagttg aaaagcaatc
    34201 tctccccaag gtgtcaatcg taaaggctca tccgctttcg ttcttccact cacagaacta
    34261 aactctgttc ccacaaaaat agcagtctta acttcaggaa tagaggttac tccagcttta
    34321 cccaaaattt gattaactcc attccactct tggtaatcct taccaccctt agcaagatga
    34381 tataacaatg ttaaagagtg agtctttcct cctccaaact gagtgctcaa attaaaaatc
    34441 ggcgaagtct caacagtgat gccattccat cgcctaacgg cttgtgaggc aaaatctaat
    34501 aagctctccg tcaaataagt tctttgaaaa aacttaactg gatccgtata atccttaccc
    34561 gcaaccccat ctcgtacatg atccaagtgc acagcaaatt cagaagcatc gagaggtctt
    34621 ccttctctta gatcttcacg ggggctaatg attgtatgcc aaggtttgag gttcatataa
    34681 attttcctta acttattcct taattaatac taagtattgc atttacttta gtttgaagta
    34741 gtttcactaa ttctggatcc atgaatttat aatcattatg aatatcgagc acatgcatct
    34801 ctttatattt tatagctctg gtaaattttg taatcatgcg ctcttcatgt tttttctcca
    34861 tgacaaaaat tatatccgcc cattgaacat cttctatggt cacttcacgc actgcatctt
    34921 tacttgttcc tgctgaacga acagaaagag attcatcatc tttatagatt ttttccgctg
    34981 ttggactacg atctctgtta ccgctacaga taaataaaat attgcgattt atcatagatt
    35041 actcagtatt acccagcttt tctctttgtt ctaattgcct gcgggtatct aaactgtgat
    35101 tgctttctac ttcacttagg aaaatatctc tatccagtct atgataccca tgataaagat
    35161 ttggggtatt aggatgagtt ttatattcta taaataattt ggtttctgaa tcataattaa
    35221 ataactctct atttggatca ccaatggcag attgtagtaa tcttatcgcc ttatcttccg
    35281 aacaaaataa tggtgattca aacttctgct gattacctct gcgttcagac atagattttt
    35341 ggtattttcc caaatatatt ataagaagtc tatccaactc ttttttatga ttagtcggat
    35401 tgagacgatc tatcggatac aaatcatgtt tatagttatg atcatatttt ctaggctgta
    35461 ccaagttgct gtaccaagaa taaaaagatt tatcaaaatt tgtaaatgga ttgctgaaag
    35521 caatttggta ccaataggat tcaattgaag tagtaagttt ttcataatca ataatagtat
    35581 aaatatttcc tccatggtaa ttttttaaag ttaccaaaaa actatattga tcaaaatgaa
    35641 aaaataaata tgccatgtaa ccaagaatgt ttttaaaagg tgaaatcttt gtcgcatagg
    35701 caaattccca tttgtaatat tttggatttt cagattctaa tttatcttcc gattcccaaa
    35761 cttccatttt ttttataaga ctgtttatta ttctaacttt attaaataga tattcttcat
    35821 gaaagtcatc atcaggaaca taacccaaat ctcgaattcg ataaatatta atattttctt
    35881 ttgagtctat atcaccaatt gtgtgaataa acgagttgag tgcttcttta ttttgttttg
    35941 ttaaatcatc tatactaata tccagaaaaa cattcaaaac tctttcactc atagtaatct
    36001 cctgaggtca agatcaaatt gatcaaagaa ttttctaggc ggtttcttaa ttcttcctgt
    36061 ctcggttaaa tttatcctct ccacatctga ataattcctt ccttcttccc gcgaaaaata
    36121 gtaaatagcc acatcttcat tggatatacc aattgtttta tctggatttt ctttcttttt
    36181 ctctttgtaa tgctcataag cggctaccat tatcccatta ataatatgat cgctatgagt
    36241 ttctacaaac acttgcacac cagaagccgc acatcgagct ataagctctg ccatcttgga
    36301 ttgtccggct gggtgcaaat gggattctgg attttcgatg ataaccatac cacctggctt
    36361 tgttgtaagt aatgcgagga taacgggtaa cacataagta agaccaaaac caacattctt
    36421 tgttttatat tcagggccat atccatcatt ttgttgaaag ctaaatgcac ctcgaatctt
    36481 tcgaatctct ttaatttcgt aggtggagag ttttgttcca ggagttattt catttaacca
    36541 caaatttgtc tgtacaccca aagaattgga gcctgataca tccaataacg ttcttgggtg
    36601 taatatatat ggattatcta gttgtgttgt cttataggat tcaaaataat ctaaagaaaa
    36661 ctttcctttg ggacctagtt cttttctttc aattacgtat tcgcttgaca tttcattttc
    36721 atcctctggt cctatcctct cagcgggtaa atattgaaaa agattatcag atttaaacaa
    36781 tggctgatca aaaaaggaag ggttaaattc agaactagaa ttcagcctta atgttcccat
    36841 tcgttctatt tgtttttttt ctaattcttc tttgtctgac tctcttaaat agtcaaagag
    36901 gaattgattt ttttcttttg aatcattaaa atagaaacca ataggagcaa attcatttaa
    36961 tactccgcta tataaggcat ccttcccagt cccaatagaa acataatctt cgtgatttaa
    37021 aaccagactt tcaagcttat tcttcaaaaa agactgccgt aacagtagaa ggctctgtaa
    37081 tgctgatgat tttcctacac cgtttatacc agtaaatata tttaagtttt ttaaactaaa
    37141 actttgtaat tcaaatgcct taaaattctg aatcgttaaa ttatttatca ttcttccact
    37201 ccatattcaa atcaggaata tatttcgcca aaatagcata aatctcttca aatctaagtg
    37261 caacgttagc cgacgaagtt gtggaagatg caatagtttc ttcaaaatct gtttctagta
    37321 atgaattaaa ttcttcaaca attttagact tgtttttgta gagaagtttt tcttgttcaa
    37381 tagtaagctt ggataaataa tatccccata catcaaacag tgctctatta aattttattg
    37441 accctctctc tgattctcta ctaaatgcct tttctccaaa aaggaatttt gctctttcta
    37501 atgatgactt aaatcgttgt tctaattcct gttttaattt cgattcattg agaagccttt
    37561 tatttaactc ctccatagct tgatctaaaa agcttcgcat agatccttta taatctcgag
    37621 ctggaataat ttgaaaagcg ataaatcgta atattacttc tctatcctgc attctttttg
    37681 cagatatttg aataacttct ttaaattctt ttgactctgc caattctttt aatagttttg
    37741 gaccttctgc ttgctgacct ttcattcttt gattcaatgc gttccgaatt tcttggttat
    37801 ttaatctcaa ccctccagta tttattctat gaaatatgct atatttcaca gcggcaggag
    37861 taacaggttt aattaaatta catgtagctt ggcattcttt aatcctgcgt ttatattcac
    37921 cagtcagttc atcataagtt ttattattca agtcttttaa gatttttaaa ccactcaatc
    37981 gtaatctttc tttttcctgt ttcaataata caaatttata gattgtgaat aatctctgta
    38041 aaccgtctac caccaaccag tatttatcgt cagttgcatc aaaataaaat gcgggtaatg
    38101 gcaaacgaag taaaatggat tcgatgaatc tactcatcat ttgatcagac caaagattcg
    38161 aattcctttg aaaatcagga ctcatatcga tttcttcgta ttcaatccta gaaattaaag
    38221 tatctaaact tctttgttca atagaaatat ttatatcctt tggatcaaaa acataatctt
    38281 caaattcttc ttcttcattt tcaacttctc ttttatattt atctgaaatt tctgtaggta
    38341 ttgcctttaa cgcttctttg cgtttagatt tattaataat tacattataa aaataataat
    38401 tatgggaatc tcttcttgcc tttggattct tatctaaaag agattttact atttcatacc
    38461 attgctcttt aattttattt ttttctatat ctaatttttg aaatgcaaaa gatacattat
    38521 taactgccgc atttttattt gatctatttt ttccctcgat tgagcgaaag cttggactag
    38581 atttataaaa atggttttct gctggttgta atatttctaa aactaattca ttataatctc
    38641 ttttaatagt tattacagcc ttattgccta tatgtaagga aaatccagat tgccctgttt
    38701 ttttaacaga taatttaatt cgaggatcat tataatctaa ttgaaaggtt ttgagcaaat
    38761 cacttagtat atcaaaaaaa tattcagcac gttgtgtatc ggcttgctca aatatttcta
    38821 aattttgttc gaatatttct ttttcaaatt tttctgtatt ttccaaaggt gccccaggta
    38881 aatctccaaa aattcccatt agctttttct tccttcttta taaatattgc agaccatccc
    38941 ctacactcct ctcctaaatg tttgcacgcc ttcaacccag cggcgttcgt ctgtgccttt
    39001 ggggtagagg gcgtttaatg attgtgctag tttccaaaag cgatcgtcat taccagcttc
    39061 ttcggataga aagcgtttta gagcttgggt tcgaccagcg gcaaatagca acatggattg
    39121 gtgcaatctg tctagcacag tctgacctgt tttttcaacc gttaactctt tccactcgtc
    39181 ggtgtccttt cctagttcgg cgggggtaaa caacgtttgt tcttttgttt ttttcttttt
    39241 attactcaaa gtttcttctt tggcttcttt gccgaagaga taattggctc tttctgctac
    39301 agggattaat cgtgaagtct cccctttgac ttcaacgaca gacgaaatac tttttagttc
    39361 agcacctaaa ccttgcgcga tacgatttgc ggtatccgag tcgagagaaa aaccgccttt
    39421 tagttttcca gccttcgcag acttaggttt atcctcatcg tcatcttctt cactagaatc
    39481 actttctgaa tcagaaccac cagctagagt ccagagccaa attgctgtta cccgtgcgtc
    39541 aggttccaga ccttctgtat ctgcatcctc gaatacgaga tgaagtgctt ctctcgaaac
    39601 cacttcccaa actttttcta gatactcacg caggttgact aaatccccat ttggtttttc
    39661 tactctttcg tattgactat aaatttccaa cgcaggacct atacaggcaa agatagcatc
    39721 cgctcctaca attccttctt tcgccatgcg aggcatccaa tctcggattc gcttaggcaa
    39781 ttctcgcaac acatcgcgcc aatcacccgt taacccagaa tccatttttt tggttccatc
    39841 caattttcct ttcccatccc aaaactctaa aggttgacga ggacggcaaa caagatgtat
    39901 agacgtagca agtgccgcag aattcattgc tcgaagtcta gaacccattt cggtatcaat
    39961 aggccatgag gcagtaatca tccaaccagc atttaacata gattgcaatt gcgcttccca
    40021 tcccgatgtt gttttattag caaacacaac aacaccaatt cccttcggag aaagaattct
    40081 atttccttcc gccatagcct tttgcatagt agattcaaaa tagtttttgt ctttgccttt
    40141 gatctcatct aaaatacatt catctgcttt atttgttagc tcttccttga ataattcagg
    40201 atagtaatta ccaaccgttc tctttaacca tacatagaaa aaatcagaaa gatcagcata
    40261 cggaactgca tcataataag gaggatctgt aaaaaaacaa tctgccgatt ggtcggctaa
    40321 tggatgtaat gcggcagagg attgcattgc agtgttgttt ttattacgta caatcacgtt
    40381 atcacacacc ataaaaatcc attcaagcat acttgctaag tttctacttg aagaacaaaa
    40441 tggatttgct tcacaaaaat cccataccat agaaattgct tggcgggcaa acaaatgtcc
    40501 taacttttct ccagtagatt gccaacttcc cattgaagaa ttgccatcag acattcgtga
    40561 taatacgaac cctaaacaag tcttaacagc cttttccaat tctagatcag ctttcgaaac
    40621 cttagcttcc tttactaatc gagtcaatgt tgtgagagta agtaactgcc tagatgtaaa
    40681 caaatccttg aattgttcca ttccataatt tctttgcgaa aaagcccttc ctgctccact
    40741 tccaccacct cttggagtta attcttgagg aactaaacta aattctcccc gatgggacgt
    40801 tatacgcttt tgtaattcct ttgtagcttt ttggattgat tgtaaatcag catccgttgg
    40861 aagtcgatag aatcttcctt gttcattaga acgagtggta aca
//
LOCUS       DAOQOS010000044        38884 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_420_length_38884_cov_5.809009, whole genome shotgun sequence.
ACCESSION   DAOQOS010000044 DAOQOS010000000
VERSION     DAOQOS010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 38884)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 38884)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38884
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_420_length_38884_cov_5.809009"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            218..451
                     /locus_tag="PLF66_11425"
     CDS             218..451
                     /locus_tag="PLF66_11425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64699.1"
                     /translation="MRKKFIYPMIFYLFSCLNRTEELRKAQKEANLCALISRSIANGN
                     QENQFIGNVFCLTELDKKVRAIDKKDYFKSDDH"
     gene            540..4085
                     /locus_tag="PLF66_11430"
     CDS             540..4085
                     /locus_tag="PLF66_11430"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HMZ64700.1"
                     /translation="MNNANQHQSPALRMEAWSINCIFVYISMYAANGNLLTVADSLPA
                     HYVEYLFLMRLILLIHIVRRTPNVLNPDGGETTASEYYDLLGRSIRKESPGVQNLSIV
                     ETTEYDAKGQVYRKSNPYLETLETPVYTTYTYNPYEGYVNRIDHPDSRYTTISYNGFN
                     ESSQTYASDASLIQSQDLVKNAKGQTLSRTIDGLTISYQYDAASRVTKVLDAMSGATT
                     TVYDDAGRRKSVTDENSGTTSYTYDLVGNIKTQTDARNITTTFTYDNLNRPTGVSYSN
                     GEGNTVLTYDEGGQAKYALGRLTTVVDAAGKLELAYDIKGNRIYQKRKIDDLEIIFRR
                     EYDLQNRLASTTYPDGTKVYQKYATTGHFSGITMDTADGSSKGYTVGSYRGPVIEDGQ
                     FKIIRETGNGVKMEIQYDPIKRRPIGLVTKLASGHIENAESYTYDQKNNITKIADTIV
                     NGRTQDFVYDAQNRLTKATGKYGVENYTYSDNGNLTQRGNFTLAYGDANHKHAVTAVT
                     SPSTGTFNYSYDEAGNMISRNGDAMSYNSQGKLKQIDTAGGDKLVYLYDSTGNRIKKT
                     SKTSKTSGVVAYSFDTFDGLSAGSLYEITRTPGEGDKHTLYFKGLYGDTFSQMTRNDA
                     ALITDASNGSKSSNLFDVALSTGILSFAKGDDSPPNLSLKSFLCSNVAGSCFDYYKNT
                     AKFYYLKGAVKVASVIYTVEYDVFVWILLVVVLYLVYLFSNKKTVIEFIEMTTSGIGQ
                     SIVTSTSSATDSWFAKRTLSVTPLLTIAILFTFTQCGFVGGKTKGNAPWLLLASGING
                     NTQSVDDPSNGSGSSGGGGGSSIVPVIGMFFLHPDHLGSITMITDGRGNLIAGGNNGG
                     KSHISYKPYGEIDRTDSSGPDISKFKFTGQEEDKESGLMYYKARYYDRMIGRFLQADS
                     MVFTDRTMGMNRYMYVEGNPVRFGDDSGHKRQNGDQERQLNYLTVAAVGFDSGGLEGA
                     ALAVAGMQNAKNRTERLGDLQITYPRHHRYSQRDALADVAHAFQRAAHDINHFNNHAI
                     PGGFKWASHGAENAAHTYSNLLFGKYHHRNSPLDQFYRTGIGRIQKKYNNYLKTAFTR
                     NRYCIRAGVETAFFIWAAYDVAAFLIFDQGAGLASPSLAGRIAALIASETGLAGPAST
                     VAVAGINVIANTNTLIGPGAQGMLNDIQHCSIKSL"
     gene            4082..4843
                     /locus_tag="PLF66_11435"
     CDS             4082..4843
                     /locus_tag="PLF66_11435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64701.1"
                     /translation="MKKIIIIFFYFLIFLNCTSQNTINEINNSNIGFSDQFVGSGRLI
                     VKFISKESVFYENVDIKITRRFPETKYQMSWESYAPLPLLLPTGKETAEPTIHEPEVQ
                     KTLEYMEGNREYNFYIKEGEYYLALESERKEYKLIDQEEKSFLFQFGFTNIVKRMPKE
                     RVVTPYNSLCESETQNYLYKNFASCPKVKIIKNRTTLIQIGVSEAIPIPIRTRIAMVF
                     GGIFFFPLRKRPFQMYFYRNMKVELLNPKVEENAE"
     gene            4833..5048
                     /locus_tag="PLF66_11440"
     CDS             4833..5048
                     /locus_tag="PLF66_11440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64702.1"
                     /translation="MQNEVRIMANYLYFQRFLCFLGKKKLIKDFLAQPSPALRIEAVS
                     INCIALEVYCYAVSRNLLIVAESLTAE"
     gene            5296..6678
                     /locus_tag="PLF66_11445"
     CDS             5296..6678
                     /locus_tag="PLF66_11445"
                     /inference="COORDINATES: protein motif:HMM:NF017183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative Ig domain-containing protein"
                     /protein_id="HMZ64703.1"
                     /translation="MKLNYKNIFYICLILFFSLNCLKAKKSNFDMRGNGLYIFSMLSG
                     NLSNSSSNSSTLNASTPPSNFTYNGTSNGLVSGSTGTSLLFRRTIAASVMPTMTGTAT
                     SITVSPSLPSGLSINATNGTISGTPTVSQVATNYTVTVANSSGSINVVISITVLPIVY
                     AATFGGGLAISSDGGASFTMKTTTDGLGCNTQNGVSVDSSGIIYAANNSNSCTGGISV
                     STNNGVSFSFLNASLNGLQNFSVFSNTPGLVYVGVYNTGGGLYYSTNGGSSFTQRFGT
                     TYLDSDYVNDIYVTSSNVYIANGFSSSYGVSISTDGGANFTKRNSGNSSLPAAVVSGI
                     YAVGSNVYAATASGLYISTNSGTSFSLSGTGAVAANKVQVVSNVIYVASTSGLMISTN
                     AGSSFSTRTTANGLPSNSVSSVFVYADGTIYVGTNSGIGISTDGGSTFSIRNASHGLT
                     NLNIQQVTVR"
     gene            6908..7183
                     /locus_tag="PLF66_11450"
     CDS             6908..7183
                     /locus_tag="PLF66_11450"
                     /inference="COORDINATES: protein motif:HMM:NF026878.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin type 47 domain-containing
                     protein"
                     /protein_id="HMZ64704.1"
                     /translation="MQESDLDWRNSGKSFEEALAEAFRRTGVDKTSFEPKEWAKDING
                     KSGVVYWKGPKGAEVSIDAPHTRRGPDVFHIGYKTQGKGNQLRGIFF"
     gene            7212..7694
                     /locus_tag="PLF66_11455"
     CDS             7212..7694
                     /locus_tag="PLF66_11455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64705.1"
                     /translation="MRNFVKESIAETLQTTGFKLDEMTPLESILFRKNILGKFTGGQD
                     FRTLWEVTVGSDFSVCDRNAWQWLDEFLLLDEFYLFFDTVDDPTINIFHENQSFTKFL
                     SEFDGYVFYLTNKNLDFLISFNDSHYLTAHGTAEPWLRNKVKELSKTGWTDASGKSYL
                     "
     gene            8206..8484
                     /gene="rpsF"
                     /locus_tag="PLF66_11460"
     CDS             8206..8484
                     /gene="rpsF"
                     /locus_tag="PLF66_11460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012389072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HMZ64706.1"
                     /translation="MRAYEITSIIVEGSQSIIDETKKAIKDILTKYSAEVTSEEDWGI
                     KKLWYSISGHESGFYSHIKCKADAKTIEKIEREFMLNQNILKSLVIRS"
     gene            8486..8917
                     /locus_tag="PLF66_11465"
     CDS             8486..8917
                     /locus_tag="PLF66_11465"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HMZ64707.1"
                     /translation="MANDLNRVTLIGRLTRDPEIKTLSNTSVCNFSIANNRVYTANGE
                     KREETHFFDCEVWGKLAEVLKQYATKGKQIAIEGRLQQQVWDAPDGKKNSKIKIRVEN
                     FQLLGGAGSSSSANQSYPQEMPVESYDPGYTSDINEDDSIF"
     gene            8988..9302
                     /gene="rpsR"
                     /locus_tag="PLF66_11470"
     CDS             8988..9302
                     /gene="rpsR"
                     /locus_tag="PLF66_11470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HMZ64708.1"
                     /translation="MKEKIYSKRDEDDEDDDKNFRGKDGEKFPKKNAKYKRKVCKFCA
                     DKTIAASLDYKRVDILERFITNRGKILPRRITGTCAKHQRQLVREIKKARSISLLPFR
                     VI"
     gene            9314..9760
                     /gene="rplI"
                     /locus_tag="PLF66_11475"
     CDS             9314..9760
                     /gene="rplI"
                     /locus_tag="PLF66_11475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017851930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HMZ64709.1"
                     /translation="MKVILQKDVTNLGEAGDQKEVADGYARNYLLPNRLAIRANEGSA
                     KVIEHQRKLAAIKKEKRVKSMQEIAKSIEGKEVQILVKVGENDKLFGSVTAIDIANAL
                     KKDGIDIDKRKIEVAEHIKALGSYEAKVKLAEGINPKLKIKVEKSE"
     gene            9767..11098
                     /gene="dnaB"
                     /locus_tag="PLF66_11480"
     CDS             9767..11098
                     /gene="dnaB"
                     /locus_tag="PLF66_11480"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010578313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HMZ64710.1"
                     /translation="MNPDPLYELESEKAFLASILLKGSPGSLELYIEPEDFYHDLHKR
                     MYASMQELIDASITVDPISLTNQLKEMSRFRDEAKDQEYIFNLYRDTVVVQPISYYAK
                     RIKKYSDRRKYIKVLRDSIEEVHAEKEDNEGLFTRIESDLAKISRQVQARGLKLVKDD
                     KSDLIDYVQKMYETKGATSGLRTHFDGLDEVTTGLKPHELIIIAARPGLGKTTFALNI
                     ASNVALKEKKIVAIFSLEMGRMELLIKMICAEARINSNSLKTGAIHPSDQKKLLESII
                     KVTSAPIWIDDSGALTIWEFKSRVRQLLITTPLSLIVVDYLQLMNDPAVKEGRQQEVA
                     SISRNLKQMAREANCPIIALSQMSRAVEQRSKEQKPQLSDLRESGAIEQDADIVAFIY
                     REDKVKDPDEIPEDQKNKAEIIIAKNRAGQTKNFPLAFTPEYSRFDNLSST"
     gene            11142..12188
                     /locus_tag="PLF66_11485"
     CDS             11142..12188
                     /locus_tag="PLF66_11485"
                     /inference="COORDINATES: protein motif:HMM:TIGR04142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HMZ64711.1"
                     /translation="MKISRFGYKFFLCFLLVNFLSLLVPAPESINRILAIVGKTSISQ
                     LDFERGEEIFKALQKQKKPGSKPPKGSLRTQVLDFLIARAIIDITADEESIQANEKNV
                     EAEIDRMMKGSGVNDRAQFEKLISERTGLPFDIWLNDLPYQIKRGQLLQVRVTIKPPS
                     DQEVQAWYNANKPKVGFEIKFREICLTPKNGSVEEEQRISSEINSIKKEARKDRDAFT
                     LIASGPRNQAAGKGINDWVPTFEIFNKSPYLVNVLGRLDDGKISDAYIDDRRRYCIVR
                     LEGKRPTPLETIRRNIQEIIQRERIDSSFDDWILTRRKEVLITVYDKEYIAENKLEAP
                     DESFNFNKIDAGSP"
     gene            12185..13702
                     /locus_tag="PLF66_11490"
     CDS             12185..13702
                     /locus_tag="PLF66_11490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001977578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spiro-SPASM protein"
                     /protein_id="HMZ64712.1"
                     /translation="MKPFDFSVSSVAIFLTSEQKHILEISDSSFFIDTFFRKLDLLFP
                     NLPIYSNFRFSNRSNIILIEVNTESDFLFAVTEKLPESISGDADFDETFFAYFIGISP
                     LISIELTQTLVQRHIRYLAQYSYSENLPKGIVPFFVSREFVSTLPEKIPATVHDYLVK
                     NLNNYDVEIFFQEPDLRQYRLDFSLSDPRSLKTSDFFLKKNPDLEYKELLTMILENPN
                     GFRLFPSYIEMEIYRGCDYKCSFCPRQFISNERDGELMSLVFVEKFLKDLESSFPYPL
                     TFCFGGMGEPLLHPDFSSILAAILNYPHTKELILETALYSNLDSLKEGLKLAGENQKK
                     LNLIINLTTLDSNQYKSIYQSELNPNDILEKISHLSSVMSPSNLHVQMIKMKEVEEEI
                     ENYFNHFEKKGINVILQKYNRYAGLMPEKRVSDLTPIHREFCWHLNRDVYINSDGNVA
                     ICRQDFNHPIGNLNTESIFTIWEKGMRIFTESLKGNHDKTMAPCLNCDEWYTFNA"
     gene            13695..15278
                     /locus_tag="PLF66_11495"
     CDS             13695..15278
                     /locus_tag="PLF66_11495"
                     /inference="COORDINATES: protein motif:HMM:NF014409.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64713.1"
                     /translation="MHEFFAFIQARSTSTRFPKKVLKTIPPESNDTILNHIIQRLKKV
                     IPSENIVLLVPKGDVEVIDYAQKNSIQYFEGSEDDVRDRFIQASKKYKAEYVIRLTAD
                     NPFIDLEYLELLMEAFEVPDLEIASFQGLPIGMGVEIFKMSSIEKELPTGLEPKHREH
                     VSLHLKESGSFRFEKFHTLLSTKEIEICSKIRLTIDEESDYKLCTEVYNKLAPKNPFF
                     GVKDVIKLYQKEKELFQINQNVNQVTFKVTHNDQKKNRIFILFAEPKEYGSGHYERCK
                     TLSVSLQSSGYNVSCAYVLPENKDYDLFIIDHRDMEIPVEIQHKTIILIDHFGQERYN
                     YFPHDLLPHMFNEFEDVVRNSLFPIGIESYKEIPEKKQVLVYAGSLNFRSSFLLDRFA
                     FKHFADAGYEIIRVGGVPRKKANFGIKTIPRTNKRDFLRMLAESEFFISYYGQSVMDA
                     SFLNKKIMIYAISDYHERLALHFSKNSEAVYVGNIYVKNMNMTAAYQKFLRKVDLNLQ
                     NEGLTILKNTIADLLKKNS"
     gene            15280..16326
                     /locus_tag="PLF66_11500"
     CDS             15280..16326
                     /locus_tag="PLF66_11500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64714.1"
                     /translation="MKQSILFLLILSLFFSVQADPYEDYENEDDKTVTADPDREKMIS
                     DAKVKLEISKLTHTALGHIKAKEWKNAEAVTKQIFELSEVSVDYFYLKGNLSYCYGEY
                     YAAIGFLNTALKYNPNHEPSHFLMGMIHSKRNEWDKAIYSFDKAAQYGSYNPYYRMNL
                     AISYFQVDNFDKAAAEARKTLELKENYLFAKILLIKSLMKVNKKEAWFYTQGLMEKNS
                     DKPNVYSLYIQLLFEYKNNYPEIIKELGKKNNLSLNEKRYLAHSYFRENDLNRSLNLF
                     KQIIASERETEDDQVIYMKILLQQGKDAEAERYLISMIRSNPSRKKHFTDIYQGLLDK
                     RDMVKGMYLPIIVR"
     gene            complement(16313..17344)
                     /locus_tag="PLF66_11505"
     CDS             complement(16313..17344)
                     /locus_tag="PLF66_11505"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:NF006083.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HMZ64715.1"
                     /translation="MIKKIGVLGAGSIGCYIGGHLLYSGCDVVFIGRERLQKEILENG
                     MTLSHFSGKKVFIEPSKIKYKTSIEELKNCDLILITVKSQDSESTAQELKNIFKDDST
                     KTLISFQNGVTNASRIESILKESKVIAGMVPYNVISKGSGIFHSGTSGKLMIQETSTI
                     SKEIVSIFQKADLEIKEHPNLEGVLWGKLIFNLNNAINALAGVPLKEELSDPMFRKIL
                     ARTMKEALSILKESKIKPVSLGKMIPWLAPIILGLPNFLFFRVASSMIKIDPEARSSM
                     WEDLNKKRKVEFDFINGEILALAKKNQIGAPIQNKIANLIKEAEEKKQGSPCYSAEKL
                     LELLNITER"
     gene            complement(17341..17691)
                     /locus_tag="PLF66_11510"
     CDS             complement(17341..17691)
                     /locus_tag="PLF66_11510"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HMZ64716.1"
                     /translation="MKIFICILVLFFSQTLTAISRADDYLLFSTEKGHLEHTKKALKM
                     GAFVNVRDSFGRTPLMFAAMFGNPDIVMLLILNGAGADFGIRDFDGKTALMYATEKNH
                     TKVIELLKSYGAIE"
     gene            complement(17771..19546)
                     /locus_tag="PLF66_11515"
     CDS             complement(17771..19546)
                     /locus_tag="PLF66_11515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64717.1"
                     /translation="MYQFTFIWKEINYITNEFIPEQKNSIQKGLNELEIETIEKEYKV
                     SLAKEVIYWFKEANGQIRPNPFWKTGLYWYSLIQCLEKQREFKRLDLYPLAEDKEGNV
                     YSAYKMEKENQWQITVNQKENLSKTWVEWGEEQFAILIKEQVLPLLTSNLREDRIRFS
                     IYARWTTYGLRIEKMIKEEWQRVLNQDILKLENWRQAESEAYLSLRRASVPQELEIVK
                     SLLAQSNPKDASPPPPSFWLGFAVIANKPNHYYCKNWLEDWFLLLYKNNSILKNWLYS
                     KNLDIIAFLSFSKRKPIQKKALDLLSKEKDGYEAIWASVRRHGEKLKQEDLEKVAKFF
                     LENDSDVLNRARIWLEDSHDSVVTLASLILLKNGRKDFIEISKAKGSKQAVKTLAQYK
                     LSSSEWKEFLRNTSHVYSWLEAICVLVKENQELVIAFSKERWVNDLANIIEGLELTHS
                     DRHRILGLIKDFPIQENNPAILKLALPLFPPGSKELYVEDKSELALYYLSCYYNHASE
                     SEKKQIQMAEDSYKKITCAKCGHNFHWNSYYTTPNVFCPNCKDKILESALGVYFYIGN
                     HARLNAYKYKPRLKQYPYNPIEDSF"
     gene            19682..20053
                     /locus_tag="PLF66_11520"
     CDS             19682..20053
                     /locus_tag="PLF66_11520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015676133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HMZ64718.1"
                     /translation="MKLAANIFTGIVMLEHVYILVLEMFLWNTPIGMKAFGFTQEFAD
                     MTKVLGANQGLYNGFLAAGLLWGFLTKDLDQSHSIKLFFVSCVAVAAIYGGYSANISI
                     LFKQGVPAFIALTLLLISGRK"
     gene            20064..20876
                     /locus_tag="PLF66_11525"
     CDS             20064..20876
                     /locus_tag="PLF66_11525"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HMZ64719.1"
                     /translation="MPKAIDENKIHQLLEKAVKGNQPALESILKEVQDFIYKLSLRML
                     WNPVDAEDATQEILIRILQNLKTFKYESKFSTWVYRVTVNFLINQKKKTYALEENYKG
                     MIRKLEKANSQIELEKKQDTEGLKLGCTYAMLLSLDRNYRIAFILHYVFELKGEEAAE
                     ILQISEVNYRKRVSRAKEKLLSFMTSRCNFINDSLTCKCPNVLTHLVSQNFITSQDTK
                     KLKSTYGSKITDKEILRNYDSMERLAILYKENSNWRSSSILNRKLKKLVLAE"
     gene            complement(20877..21326)
                     /locus_tag="PLF66_11530"
     CDS             complement(20877..21326)
                     /locus_tag="PLF66_11530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64720.1"
                     /translation="MQKRKYLLQPKKYHFQLLNKQTVQFILVKQGRMFSTGIGAASDD
                     KNKGYRLSVYELFDLKDYSSKELIEKFTVTEKIFLREIQDTANFYLIEIEMSTENPNG
                     SVVELVHGFAPIQLNAINKTNQGNPDINSGLCSFGNCGIIRRVDWGK"
     gene            complement(21431..22315)
                     /locus_tag="PLF66_11535"
     CDS             complement(21431..22315)
                     /locus_tag="PLF66_11535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64721.1"
                     /translation="MIKFKRNLKETVFQYKYIILTLVLIFSLGLVGFFYYNQKLKVRP
                     PEEDVQFNFTIDGKNYIMPSVVHSFHFLNSMGFPWQGLTESNPTHDYDNETQRALNLG
                     GRSADGTVLVFADEKIKAIEARGIFLKLADSLEITEQIKDRLFDLDTALIVHADQSVL
                     DERIIHLEYEIEKALHDKKKDHLAVLMELGAWLEGLHIASKGIINNYKPMYAEILRQP
                     HIAQIYLDIMEALIHHAKTEKEKKLLKSISSHLEQAAKIANHSHKESFPIEKVKELYE
                     ISIQIKTLIESNGETVKP"
     gene            complement(22318..22935)
                     /locus_tag="PLF66_11540"
     CDS             complement(22318..22935)
                     /locus_tag="PLF66_11540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64722.1"
                     /translation="MNKSIAILLIVLSPICSMAILPGRSLSANVSKVVNRLKEKNYTI
                     EGGRRFHIGLKKNESVRYVITVPVFLSKTAIGIATDENVNKVRVVIFSQISSEGKERA
                     IEGEDIESSNYIKEITDKSYHYIVEVTLLDTKLDQGYSSVDVLVAYAPMQLATKEDVK
                     DFYYEHTGEIEYYSDPIRAKQAGYRKSDTGRIPCSAIDYGRTCVR"
     gene            23238..24542
                     /locus_tag="PLF66_11545"
     CDS             23238..24542
                     /locus_tag="PLF66_11545"
                     /inference="COORDINATES: protein motif:HMM:NF021778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HMZ64723.1"
                     /translation="MLGFGIFKKIQISFALNRIDLDYKSFILFSLFLGFCNPAEFENP
                     CDPKSETFRKNYLIISDLGIKSGYCGISSTPFSNGSNSNTGTNQTPTIPTKVAKFLMV
                     SNAGSNNVNVYTINSTTGALTQISGSPFAAGGGARFVTAHPSGLFAYVANGSGSSISA
                     YLINTTTGFLTPISGSPFAIPVNPYALTINAAGTLLYVAPYSGTLIYMYSINLTTGAL
                     TFVNSFSNTGVYAASLAIDPTGKYMYAGDTTNPGRIDAFQIDSSNGFLTPIAGSPYIN
                     GGNDTIGVTVDPTGKYVYATNYFTNDIYGYVLNPTTGQLSGIPGSPFATGSSAPTFII
                     VEATGKFLYVPNSGTNNISGFTVNQTTGALTSISGSPFLCGASPSVVYADPSSTFLYS
                     ANTSGNNVSGFTINSTTGALTPIAGSPFAAGTSPFGFGFVSY"
     gene            24573..28136
                     /locus_tag="PLF66_11550"
     CDS             24573..28136
                     /locus_tag="PLF66_11550"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HMZ64724.1"
                     /translation="MLRILLIGLFVLAVNTFAEDSAKKSWTQKKRVALIVGVNEYKDT
                     TSLKYPVSDSKKMKTIFEKVGYFDHIEILNDEQGKADNTKLPTKENIENTFKALIALK
                     PDTILFYFSGHGLIDGEKNNAIAPSDLKVSKDFKKIENTINLDELAKQVDTSIEQGIF
                     LIDACRSPLPKGERISLDKANVSETNLKEKQILKPFSWKKKLTFSTETIREDVENARG
                     VTVLIGTSPDNSSLEDDSIKSGIFTYYMIKGLEGGILDENIEEYVTMSSLRSYIDKKF
                     IAHKQENLQILEENLKKTKKDSENYQILQEKVLETKKSLPQKTYLIAPARGVTGDVMI
                     SFGRPNLDNLIERVKYFDDDGILIHAKVHLENGSRKSYMNFFSKQESTGKFYPDDLDG
                     VGKMKFTYDENYFEARQFDKEGRETFTHKLEYDPDKKNWIYAGVVSKSDIGAKFEKVT
                     FDTKGKILKKGYFDWNSENGFISYIGGITYTYDSLGKIKEELNAVKGRDYTVKYFYNS
                     KGLLISKKIYRFDNAKNKDILYEDENNTHEYKYSYDSSGNLILEEYFNLKGKLQDDLN
                     NIAYKKYSYDKSGNKIEEEAFRSTHNPLSKEGVTSTVFKYDSKKRVIEKSFFAEAEVD
                     SNKKKIKQIDDFGVHAYRYTYDEECLRDRSIAIRRRRDFYQEGVDWQANAKTSENCYK
                     SVEMLDRSGSPTPNRLGATKIEFINDGGRELFKRYLNSSGKFLYADIPHQVYYANRFD
                     DKGNHIERDLRLVSGELLENQDGFAGIHFKYDQDRREIERIYIGANGKPVTGIRGVAR
                     YVNTYNKNGKLISVEMFDPKGKLVAEDESKVAKEIYKYNEKDQLVLTEYYGVDGKLFS
                     DGGLAREKRDYDPNGNVILIEEYSSANKLITKEKNQYNPKNQITRKEIWNEKELVLNK
                     EYFYDKENRLIEENSGSEKTKYTYLDPTHFKEEKELNGQKVPVFGNAFSVLYELNSSG
                     KIISKTYLDESGKNPINQKRMPSVYRYEYDGDGNLILEECLDFYGNLVDPNSNLEPAR
                     KIFRYDANRNKIFETYFREDGWLFNIIRNGLSYSRHTIEYDLECARDFINRNKSKNQS
                     EIEVMLREFSGCEIFRSYHKSNGELLQRYTRDKQNNEYLSMEKVDAKGYISVGGERGI
                     QKDTYIQLFENPEGNSNSKRIRLTLDRFHRPIGISFEAGKR"
     gene            complement(28313..29581)
                     /locus_tag="PLF66_11555"
     CDS             complement(28313..29581)
                     /locus_tag="PLF66_11555"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006863552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HMZ64725.1"
                     /translation="MGISKKTIRDMELNGKRVVMRVDFNVPIKNGAIQDDTRITAALP
                     SIKYILDKGAKSLVLMSHLGDPKKDMKKAKEKAEKAGKPFNEEDYINGQHKMAPIGAH
                     LAKLLGREVKVTDSCIGESVLSTVNALSNGQVVLLENTRFHKEETSESAEDREKLAKE
                     LAKYGDIYVNDAFGTAHRAHASTETLAKYLPAVAGFLMEKELEFLEEKVVKNPAKPFV
                     AIVGGAKVSSKITVIEKLMDKVDKIIIGGGMAFTFFKAMGKEVGTSLVELDQLEVAKH
                     TMAKAKEKNIEFILQTDAIVASAFANDANKKTVDIDQIPADMMGLDIGPKSIDLFKSA
                     LSNAKTVFWNGPVGVFEMESYAKGTVEIANSLASLSNAITVIGGGDSVSAVNKAGVSD
                     KMSHISTGGGASMELVEGKVLPGVAALNNK"
     gene            complement(29615..30562)
                     /locus_tag="PLF66_11560"
     CDS             complement(29615..30562)
                     /locus_tag="PLF66_11560"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004750345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HMZ64726.1"
                     /translation="MIDRISIPFLEGIWGGPSTFSILMLIAFLTGSKLLPLEYKRKGL
                     VPEAADTIVFLGVLGTLVGAKIFYIFEIWDQIFVVPGMFSYPWTHWNGFQELNDPSCE
                     VCRTSMGLWSSLFNGGGLVFYGGFLFGTLFIYFYLKWNEYDLGKYYDGMASTMAIGYA
                     IGRLGCFVSGDGCYGFHTDVNIPILVFNFHGAHPSGVPVWNTPVIEAIISFLYFLYFH
                     YWARFQNFKKWSIFFQYIALHGLARLCIEFLRVNKAVFPFIEPPKMVNIPDASGNPTF
                     LTGYYWHGFSQSQYISIAFILVSVIFFIKMRLWEKETAN"
     gene            complement(30609..31628)
                     /locus_tag="PLF66_11565"
     CDS             complement(30609..31628)
                     /locus_tag="PLF66_11565"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HMZ64727.1"
                     /translation="MELSENKLTLLERMTLKYYRLQKSFQKLHIFGKTKVSLLLIPHS
                     NENIVKVEISHYSLGFLALLITSLFIMGISFLGYYIYTRNDHVDLYSRGNTEKVFFLH
                     HDLMGDILEDTIEDLASETEDLNKVTWERSLRDSKAKEELGLMQETAEKANINPETEL
                     DSNMKIFIYTVEKFANVYESLSTLKANFQNSMDYLETRESIFESMPRGRPLGPGVGLI
                     TSTYGHREDPFYVDVGEFHNGIDFASANGTPIYATAPGIVADASGSDGGLGLHVRINH
                     ENGFFTMYGHCSKLLVAEGDVVKQGDIIALVGSTGKATGSHLHYEVHIGLDPPINPQE
                     FINID"
     gene            31734..33101
                     /gene="pcnB"
                     /locus_tag="PLF66_11570"
                     /pseudo
     CDS             31734..33101
                     /gene="pcnB"
                     /locus_tag="PLF66_11570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000670771.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004652 - polynucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006378 - mRNA polyadenylation [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
     gene            33455..34249
                     /locus_tag="PLF66_11575"
     CDS             33455..34249
                     /locus_tag="PLF66_11575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64728.1"
                     /translation="MSQDRHSFKSLLGFVLIAYLFLVSFCLLAEEKIDSMILHEPKDE
                     TQYGSNIRRAEQTLNQTISKLYLEISSYSNLLNLKIKSLPHRTLVKKGTAKGEDCIIS
                     TTEKDVDCISIEQFDFIEGSFGEAKGTKSKLIVLFFDQKASADNKDLSPKLKKVKSRI
                     YSHNYYSQDKTWMEVVDNDPLGNPDHDDKIIIRSESNPYFSSTPVKTEEVGNRESYSL
                     KSIENTKSHPIRNHFKREAYSKHLQLFEKLLARILNFNLKTENNKN"
     gene            complement(34253..35038)
                     /locus_tag="PLF66_11580"
     CDS             complement(34253..35038)
                     /locus_tag="PLF66_11580"
                     /inference="COORDINATES: protein motif:HMM:NF037627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HMZ64729.1"
                     /translation="MPTKIKKDSFRKLLRFWRKQKRISQLSLSLDLEISSKHLSFLET
                     GKSNPSRSLVLKIADYFKMPFRQKNLFLISAGYSPEYSEESFYSDKLKIIRDAFLQIL
                     DKHERYPALVVDTSYQILMKNKGYEQIVKTFAGINAFHQFENSIELFLSPDGFRNYLQ
                     NLSQLESFILNRLKEEMNFSQSKKLFNLYHKISRHPRAKEVFSTQEEMNLPVLKFTLQ
                     KENKKASFFTILSTLGTPTDVTSQELRLELLFPVDEETKKLFL"
     gene            35120..35512
                     /locus_tag="PLF66_11585"
     CDS             35120..35512
                     /locus_tag="PLF66_11585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64730.1"
                     /translation="MQITQILPYLAGGFLAIYLIFTIISKEKSKIGWIFPAFLSLLFF
                     SYSVFTVSQEGPIGFWVEHTRNFWGNQIWFDLLLAASIGWYFILPKARELKMNLPFWA
                     FAIVATGSIGFTAMVSRLLYLEEKREKQ"
     gene            complement(35624..36058)
                     /gene="ssb"
                     /locus_tag="PLF66_11590"
     CDS             complement(35624..36058)
                     /gene="ssb"
                     /locus_tag="PLF66_11590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015678373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HMZ64731.1"
                     /translation="MANDLNNVVLIGRMTKDAEYKTVGQTSLANFSIANNRTYVDNTG
                     TRKDLVNYFECTVWGKLAEVLKQYGGKGKQLMVEGRLEQSTWDAPDGKKMSKVKIRVE
                     NFQLLGGRSDSGASSSTDTNKMTSHPDPFPSDIDEIPADDIF"
     gene            36421..36825
                     /locus_tag="PLF66_11595"
     CDS             36421..36825
                     /locus_tag="PLF66_11595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64732.1"
                     /translation="MKIIAISLYILSGLLFFYSLTQPVFLFSDHEPIEGLHVLFWGWW
                     GMILGHPGWLANWLYSATITMNLLGKRKIAIILGLIAVAFSLDGFRVTEWYFNEGNST
                     PIKDLGVGYSYWLAAIGVGAFSTLFSTNQFID"
     gene            complement(36815..37468)
                     /locus_tag="PLF66_11600"
     CDS             complement(36815..37468)
                     /locus_tag="PLF66_11600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004787949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HMZ64733.1"
                     /translation="MNEANIKVNEYLVRGCGRCKLYDTPDCKVHNWEQELKALRKILL
                     ETELVEEIKWSMPCYTHNKKNILILAAFKEYCSVNFFKGSLLKDPKMILETAGDNSQS
                     GRLIRFTNLKDVKTLESSLKEYIQEAIKIEESGKKVKTKDVSEYNVPEELESIFKKDS
                     VFKKAFQALTPGRQKGYLIYFSQPKQSKTRIDRIKKYRPLILEGKGFNDSYKEKINR"
     gene            37629..38852
                     /locus_tag="PLF66_11605"
     CDS             37629..38852
                     /locus_tag="PLF66_11605"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HMZ64734.1"
                     /translation="MKDVEKLKIDSEFGAHTYSLNYARVQLFNKVFFICNLIVILLNI
                     VNYSKGIGNKNPYSQLYIPAYFFYLVYFFIMSLITFYLKWQPADKIRWFHKVNVYLFA
                     AMMLVWINVITFIDLRVNGGITVYALGLLGMASILFLNPIRAVIFYIISAIAFYTLLK
                     THNMPEDKIIADSVNGTIMIVVSFIISQIIFMEKKKQFIKDIQIQKQQSDLEKINSDL
                     QSQNLKNETLQMIIKNYIPWTTWKKADLNSTLSLPKIPEEEIEITCLFLDIVNFTVFS
                     EKNSPKVVIEKLNKVFRPIVATIYRNNGDIDKFIGDAVFAVFEDPKQALKSGFEIFHL
                     LREADLESMDDIRIGIHTGRVIRGDVGGEFRRDNTLIGDTVNIASRLQTSCLPNELII
                     SETSANLCQNQSVAK"
BASE COUNT        12987 a         6350 c         7070 g        12477 t
ORIGIN      
        1 ttagtctcct tttggaagcc taaaatcgtg tccttttttt atgtaggggt tcaaaactgg
       61 gcgacgttag atatcaaaaa attcaagaac atcgcgccta tattaaggct cttggctgta
      121 cttatgcatc aagagtagct ttttctggag atcaagattt acaaacgttt ggttttgttc
      181 attgtcttaa cgaatacata aaggataggg attaattatg agaaaaaaat ttatctatcc
      241 aatgattttt tatttgtttt cttgtctcaa tcggacggaa gaacttcgta aagcacaaaa
      301 agaggctaat ttgtgcgctt taatttcaag aagcattgct aatggaaatc aggaaaatca
      361 attcattggt aatgtattct gcttaactga attagataag aaagtccgag ctatcgataa
      421 gaaggactat tttaagtctg atgatcatta atgcagtttg aaagagaacg ggtaatataa
      481 gtgatgcatc aaaaaattaa tgagcagaat ttaaaaagga taaaagcaac ggaacacaaa
      541 tgaataacgc aaaccaacac caaagccccg cgttaaggat ggaagcgtgg tcaataaatt
      601 gcatattcgt ttacatttcc atgtatgcgg ctaatggcaa tttattgacc gtagctgaca
      661 gcctgccggc gcattacgtt gagtatctct tcttaatgcg tttaattctg cttatccaca
      721 tagtccgccg aacgcccaat gtattaaacc cagacggagg agaaacaaca gcaagtgaat
      781 actatgactt attaggaaga agcataagaa aagaatcacc tggtgtgcaa aatctttcaa
      841 tcgtagagac aacagagtat gatgcgaaag gtcaagttta tagaaagtcc aatccgtatc
      901 tagaaacctt agaaacacct gtgtatacaa cttacacata caatccatat gaaggttatg
      961 taaaccgaat cgatcatcca gatagcaggt atacaacaat ctcttataat gggtttaatg
     1021 aatcttccca gacctatgcg agtgatgcaa gcctaatcca atcacaagat ttagtaaaaa
     1081 atgcgaaagg gcaaactcta tcaagaacga tagacggatt gacaattagt tatcaatatg
     1141 atgcagcttc gcgtgtgaca aaagtattag atgcaatgag tggagcgact acaactgtat
     1201 atgatgatgc aggtagaaga aaatctgtga ctgatgaaaa ttctggaaca acaagttata
     1261 cttatgacct agttggaaat atcaagacac aaacagatgc aagaaatatc acaactacat
     1321 ttacttatga caacttgaat agaccgacgg gtgtaagtta cagtaacgga gagggtaata
     1381 cagtattaac ctacgatgag gggggacaag cgaagtatgc gctaggtcga ttgacgactg
     1441 tagtagatgc tgctggtaaa ttagaattag cctatgacat caagggaaat cgaatttacc
     1501 agaagagaaa gatagatgat ctagaaatta tctttagaag agagtatgat ttgcagaata
     1561 gacttgcgtc tactacttat ccagatggaa caaaggtata tcagaagtat gcgacgactg
     1621 gacatttcag tggaatcact atggatactg cggatggtag ctcgaaaggt tacactgtag
     1681 gttcttaccg aggacctgtg atagaggacg ggcaatttaa gattatccgc gagactggta
     1741 atggcgtaaa gatggaaatt caatacgatc caattaaacg cagaccaatc ggacttgtga
     1801 caaagctagc gagtggacat attgaaaatg cggagtcgta tacatacgac cagaagaata
     1861 atattacaaa gattgcagac acaatcgtga atggacgaac acaggatttt gtttacgatg
     1921 cgcagaatcg tttgactaaa gcaaccggaa agtatggagt tgagaattat acttattctg
     1981 ataatgggaa tctaacccag agaggtaact tcacattagc ctatggagat gcaaatcata
     2041 aacatgcagt tactgctgta actagcccat caacaggaac atttaattat tcttatgatg
     2101 aagcgggtaa tatgattagt cgaaatggtg atgcgatgag ttataactcg cagggtaagc
     2161 taaagcaaat agatactgct ggaggagata aattagttta tctttatgat tcaactggaa
     2221 atagaattaa gaagacaagt aagacaagta agacaagtgg agtcgttgca tatagctttg
     2281 acacattcga cgggctcagt gcaggctcat tatacgaaat aacccgcaca ccaggagaag
     2341 gggataaaca tacgttatac ttcaaaggct tgtatggtga tacattcagt cagatgacaa
     2401 ggaatgatgc ggctttaata acagatgctt ctaatggtag taaatcctct aatcttttcg
     2461 atgttgcttt atctacagga atactctcct ttgcgaaagg agatgattcc ccccccaatc
     2521 tttctctaaa aagtttctta tgttctaatg tcgcaggttc ttgttttgat tattacaaga
     2581 atacagctaa gttttattat ttaaaaggtg cggtgaaagt agcaagtgta atatacactg
     2641 tcgagtatga cgtgtttgtt tggatactat tggttgtggt gttgtatttg gtttatttat
     2701 tcagtaataa aaaaacggtc attgagttta tcgaaatgac aactagtgga attggtcaga
     2761 gtattgtcac ttcgacaagc tcagcgaccg attcttggtt cgccaaacgc acattatccg
     2821 taacaccgtt attaacaata gcaattttat ttaccttcac acagtgcggg tttgtaggag
     2881 gaaagacgaa aggaaatgcg ccgtggttac tacttgcaag tggaatcaat ggaaatacac
     2941 agagtgtaga tgacccttcg aatggatcag gttcctctgg tggtggcggt ggatcgtcaa
     3001 tagttccagt aattggaatg ttctttttac atccagatca tttgggtagt ataacgatga
     3061 taactgacgg tcgtggtaac ttaatagctg gagggaataa cggtggtaag tcccatatat
     3121 cttacaaacc gtatggtgag atagatagaa ctgactcctc tggtcctgat atatctaagt
     3181 ttaaattcac tggtcaggaa gaagataaag agtctgggtt aatgtattac aaagcgagat
     3241 actatgatag gatgattggt agattcttac aagcagatag tatggtattt acagatcgaa
     3301 caatgggtat gaatcggtat atgtatgtgg aagggaatcc ggtgagattt ggagacgata
     3361 gtgggcataa acgtcagaat ggagatcaag agaggcaatt gaattattta actgttgccg
     3421 cagttggttt tgatagtggg ggacttgaag gtgcagcact tgcggtggca ggtatgcaaa
     3481 atgctaaaaa cagaacggaa agactgggag accttcagat aacctatcct aggcaccatc
     3541 gatattcgca acgagatgct ttagcagatg tcgcacatgc gtttcaaaga gcagcccatg
     3601 acattaatca ttttaataac catgcaatac cgggtggttt taaatgggca agtcacggag
     3661 cagaaaatgc agcacatact tatagcaatt tattatttgg aaaataccat catagaaatt
     3721 cgcctttaga tcaattctat agaactggta taggtaggat tcaaaagaaa tacaataatt
     3781 acttaaagac agcctttact agaaataggt attgcattcg agctggagtt gagaccgctt
     3841 tttttatttg ggcggcatat gatgttgcag cgttcttaat ttttgatcag ggggcaggat
     3901 tggcatcacc aagtcttgca ggcaggatcg ctgcattaat agcaagtgaa acaggtttag
     3961 caggtccagc atctaccgtt gcagtggctg gaataaatgt tattgctaat actaacacat
     4021 tgattggacc gggtgctcag ggaatgctta atgatataca acattgttcg ataaaaagtt
     4081 tatgaaaaag ataatcatca tattttttta ttttttaata tttcttaact gtacaagcca
     4141 aaatacaata aatgaaataa ataattctaa cattggattt agtgatcagt ttgttggtag
     4201 cggacgactg atagtaaaat ttatttccaa agaatctgtt ttttatgaaa atgtagatat
     4261 taaaattact agaagatttc ctgaaacaaa atatcaaatg tcttgggaaa gttatgctcc
     4321 attaccgctt ttgttaccaa ctggaaaaga gacagcagaa ccaacaattc atgaaccaga
     4381 agtgcaaaag acacttgagt atatggaagg taacagagag tataatttct atataaaaga
     4441 gggagaatat tatttagcac ttgaatccga aagaaaagaa tataaattaa ttgaccaaga
     4501 agaaaaatct ttcctatttc aatttggttt tacaaatata gtaaaaagga tgccaaaaga
     4561 gagagttgta actccatata attcgctttg tgaaagtgaa acgcagaatt atttatataa
     4621 aaattttgcc agttgtccta aagtgaagat tataaagaat cgcacaacat tgattcaaat
     4681 tggtgtttca gaagccatac caataccaat acgtactaga attgccatgg tctttggtgg
     4741 gatatttttt tttccacttc ggaagcgtcc ttttcaaatg tatttctatc gaaatatgaa
     4801 agttgaattg ctaaatccta aggttgaaga aaatgcagaa tgaagtaaga attatggcaa
     4861 attatttata ctttcaaaga tttctttgct ttctaggtaa gaaaaaatta ataaaggatt
     4921 ttctagcaca accaagcccc gcgttaagga ttgaggcggt atcaataaat tgcatagcac
     4981 tagaagtcta ttgctatgca gtaagtcgca atttattgat agtagccgaa agcctgacgg
     5041 cagaatagta ttatgcgctg cttaggatag ctaatttcct ttacacgcga gaggaccggg
     5101 acgcccgaaa aaaagcaaag agtagaggaa agaagctatt cagaaataat ttattattta
     5161 attcttttac tggattcata attttttctg ctttttctca gaatctacta aaaaataatg
     5221 tggataatcg ctgtaaacga acacctaaaa ttgcaaaaga gtgtgttttc gtttgtttat
     5281 taaggttgaa ctattatgaa actaaattat aaaaatatat tttatatatg cctaattctt
     5341 ttcttttctc taaactgttt gaaagctaag aaatctaact ttgatatgag gggaaacgga
     5401 ttatatattt tttctatgct ctctgggaat ttgtctaatt caagttctaa ttcttctact
     5461 ttgaatgcct caacgccccc ttctaatttt acctacaatg gaacaagtaa tggacttgtg
     5521 tctggatcaa caggaacttc tcttttgttc agaagaacaa tcgcagcttc tgtaatgcct
     5581 accatgactg gaacggctac ttctatcaca gtttctcctt ctcttccttc tggtcttagc
     5641 atcaatgcaa caaatggaac catttctggg actccgaccg tatcacaagt agctaccaat
     5701 tatacagtca cagttgcgaa ttcttcaggt tcaatcaatg tagtgatctc tatcactgtg
     5761 ttgcctatcg tctacgcggc aacctttgga ggaggtcttg ccatctcgtc cgatggtggt
     5821 gcgagtttta caatgaaaac aactacagat ggacttgggt gtaatactca aaatggagtg
     5881 tctgtcgatt cttctggaat tatctatgca gcaaataatt cgaatagttg cacaggagga
     5941 atttctgtat caacgaacaa tggagtttct ttttcttttt taaatgcatc gcttaatggc
     6001 ttacaaaatt tttctgtatt tagtaatact cctggattag tttacgttgg agtttataat
     6061 acaggcggtg gactttatta ttcaacgaat ggtggttcta gttttacaca aagatttggg
     6121 actacctatt tagattcaga ttatgtaaat gatatttatg taactagttc gaatgtttac
     6181 atagcgaatg gattttcttc ctcgtatgga gtttctattt cgactgacgg tggtgcaaat
     6241 tttacaaaga gaaatagtgg aaatagttct cttcctgctg ctgtagtcag tggaatttat
     6301 gcggttggat caaatgtcta tgcggcgact gcaagtggtt tgtatatctc taccaatagt
     6361 ggcacatcct tttctctttc cggaactggt gctgttgcag cgaacaaagt ccaagttgta
     6421 tccaatgtga tctatgtagc atctacaagt ggacttatga tatcaaccaa tgcaggttct
     6481 agtttttcta caagaactac agccaatggt cttcctagta actctgtcag ttctgtgttt
     6541 gtttatgcgg acggaactat ctatgtggga accaattcgg gaattggtat atcgactgac
     6601 ggtggttcta cattttctat aagaaatgcc tctcacggtt tgactaattt aaatattcaa
     6661 caagtcacag ttagatgaaa tgtaaaagtg gcaaacgggc ggaacgcacg aaagatcgcg
     6721 aagtttgtaa accctctgtg ctcttcgtgg tgaagaagaa ttataaatgc ataacgtata
     6781 attgtttata gaatcctaaa aagaatatct attaaattcg gacaccaaaa catacttgaa
     6841 agaataaata gaaaaaatag atttaattaa tgaattttat agtagaacca aaaataaaaa
     6901 cagaagaatt caagaaagtg atttagattg gagaaattct ggtaaatctt ttgaggaagc
     6961 attggcagaa gcatttcgaa gaactggtgt tgataagact agttttgaac caaaggaatg
     7021 ggcaaaagat ataaatggta agtcgggggt agtttattgg aagggtccga aaggagcgga
     7081 agtaagtatc gatgctccac atacaagaag aggaccggat gtttttcata ttggctataa
     7141 aacgcaaggt aaaggcaatc aactacgggg catatttttt tagaatgtgt tccctttttt
     7201 aggaaagtag aatgagaaat tttgtaaaag aaagcatcgc tgaaacatta caaacaacag
     7261 gtttcaagct ggatgaaatg actccgcttg aatctattct atttagaaaa aatattctgg
     7321 gaaaatttac tggtggtcag gattttagaa cgctttggga agttactgtt ggttctgatt
     7381 tttctgtatg tgatagaaat gcatggcaat ggctcgatga atttttatta ttagatgagt
     7441 tctatctttt ttttgataca gttgatgacc caactatcaa tatttttcat gaaaatcaat
     7501 ccttcacaaa gtttctttca gaatttgatg gatatgtatt ctatctcaca aataaaaatc
     7561 ttgattttct tatttctttt aacgatagtc actatctaac ggcgcatggc acagccgagc
     7621 cttggcttcg caataaagta aaagaacttt ctaaaacagg ttggacagat gcatctggaa
     7681 agagttatct gtaattcttt tttggctttc aatacaaaac aatatcagag catttttaat
     7741 tatcctttct actcttcgtt tgaatccgaa tggaagtgtc gaagttcggg aagaaaaata
     7801 ggaatggaaa taaataacgt aatttcgatg tgacagaaaa tttaaaagat tgccacggat
     7861 aaaataataa ttattaaaac catccgcatt tatctgtgtt catccgtgtc tatccgtggt
     7921 taaatatata tgtttaactg taaaaatgat ttgtaagtgg ttaaattagc tgtgaatttg
     7981 accacttgag tagcaggggg ctgaaggctg aaatcccaaa aaaagaatag aagtcgatcc
     8041 ttttgctctt tatccaccgc atcctccaaa tgctttcaaa ctccttgacg cgaagcccga
     8101 ccgttttagt ctggtataaa ttcaaaagct tctaccctta ctgtctcaat ttttcgcaaa
     8161 aattgaatta gaacagaact aagggttaac ccaaaggaga aaattatgcg agcgtatgaa
     8221 attacgtcta tcatagttga gggatctcaa tctattatag atgaaactaa aaaagcgatt
     8281 aaggatattt taactaaata ttctgctgaa gtcacttccg aagaagactg gggaatcaaa
     8341 aaactctggt attccatttc tggtcatgaa tctggttttt acagccacat caaatgtaaa
     8401 gccgatgcaa aaacaatcga aaagattgag cgcgaattca tgctcaacca aaacatatta
     8461 aaatcactcg tcattcgttc ctagtatggc aaatgacctg aatcgagtca ccctcattgg
     8521 tagactcacc cgtgatccag agattaaaac tctttccaat acttctgttt gcaactttag
     8581 tatagcaaat aacagagtgt ataccgcaaa cggagaaaaa agagaagaaa ctcatttctt
     8641 tgattgtgaa gtatggggca aattagcaga agttctaaag caatatgcta caaaaggcaa
     8701 acagattgcg attgaaggac gtttgcaaca acaagtttgg gatgcaccag acggaaagaa
     8761 aaatagcaag atcaaaattc gggtggagaa tttccaatta ttaggtggtg ctggttcgag
     8821 ttcttcggct aatcaatctt atccgcaaga aatgccagtt gaaagctatg atccaggtta
     8881 tacatccgat atcaacgaag atgattcaat tttttaaaaa ctaaactatt aaaaggacta
     8941 ttactatgtc agaacaagaa atgaaagaca ttcaaccaga ttatcaaatg aaagaaaaga
     9001 tttacagcaa acgcgatgaa gacgatgaag atgatgataa aaacttcaga ggaaaagatg
     9061 gcgagaagtt tcctaagaaa aatgctaaat acaaacgtaa agtttgtaaa ttctgcgcag
     9121 acaaaacaat cgcagcaagt ttagattata aacgagtaga tatcttagaa agattcatta
     9181 caaaccgtgg taaaattctt ccaagaagaa tcacaggaac ttgtgcaaag caccaaagac
     9241 aattagttcg tgaaatcaaa aaagcaagat ctatttctct cttaccattt agagtaatat
     9301 aaggagtcat tcgatgaaag ttattttaca aaaagatgtt acaaatttag gcgaagcagg
     9361 tgatcaaaaa gaagtagcag atggatatgc aagaaactat ctacttccaa atcgattagc
     9421 aattcgtgca aatgaaggat ctgcaaaggt aatcgaacac caaagaaaac tcgctgctat
     9481 caaaaaagag aagcgtgtta aatctatgca agaaattgca aagtctatcg aaggaaaaga
     9541 agtgcaaatt cttgttaaag ttggagagaa cgataaacta tttggatctg ttactgcaat
     9601 tgatatcgca aatgctctca aaaaagatgg aattgatatc gataagcgca agattgaagt
     9661 tgctgagcat atcaaagcac ttggtagtta tgaagcaaaa gtcaaacttg ctgaaggtat
     9721 taacccaaaa ctaaaaatta aggtagaaaa atcagaataa ttttctatga atccagatcc
     9781 actttatgag ttagaatctg agaaagcttt tttagcttct atacttttaa agggatctcc
     9841 cggttctttg gaattataca ttgaaccgga ggatttctat cacgatctcc acaaaagaat
     9901 gtatgcatct atgcaggaac tcattgatgc atcgatcact gtagatccta tctcactgac
     9961 aaaccaatta aaagaaatgt ctcgtttcag agacgaagcc aaagatcagg aatatatttt
    10021 taatctctac cgagataccg ttgttgtgca accgatttcc tattatgcaa agcgtatcaa
    10081 aaaatactcg gaccgaagaa aatatataaa agttctccgt gattctattg aagaagttca
    10141 tgcagaaaaa gaagacaatg aagggctttt tactcgaatc gaaagtgatc ttgccaagat
    10201 ctctaggcag gtgcaagcga gaggtttaaa attagtaaaa gatgataagt cagacttaat
    10261 tgattacgtt caaaaaatgt atgagaccaa aggtgcaacg agtggactta gaactcactt
    10321 tgatggactc gatgaagtaa ctactggtct gaaacctcac gaattaatca tcattgcagc
    10381 aagacctggt cttgggaaaa caacatttgc cttaaacata gcatctaacg ttgctctaaa
    10441 agaaaaaaag atagtagcta tcttttctct tgagatgggg cgcatggaat tactcattaa
    10501 gatgatttgt gcagaggcta gaattaattc gaatagttta aagacaggtg caattcatcc
    10561 aagcgaccaa aaaaaacttt tagaatctat tatcaaagta acctccgctc cgatttggat
    10621 tgatgattcg ggtgctctta ccatctggga gtttaaaagt agggtaagac aattgcttat
    10681 tactacgccg ctttctttaa ttgtagtaga ttacttgcag ctcatgaatg atccagcggt
    10741 caaagaagga agacaacaag aggttgcttc tatttctaga aatttaaaac agatggcaag
    10801 agaagccaac tgtcctatca ttgcactttc ccaaatgtct cgagcggtag agcaaagatc
    10861 caaagaacaa aagccccaac tttctgactt aagggaatct ggagcgattg aacaggatgc
    10921 tgatattgtt gcctttattt atcgagaaga taaagtaaaa gacccagatg aaattcctga
    10981 agaccaaaag aacaaagcag aaattataat cgcaaaaaac agagctggac aaacaaagaa
    11041 ctttcctcta gctttcactc ccgaatacag tcgttttgat aatctaagca gtacttaaaa
    11101 aacaattgaa aaaaacgata ccttctctat aacataattc tatgaaaatt tctagatttg
    11161 gatataaatt ttttctatgt tttctcttag ttaatttttt atctcttcta gttcctgctc
    11221 ctgaatctat taatcgtatt cttgctattg tagggaagac atctatttcc caacttgact
    11281 ttgaaagagg agaggaaatt tttaaagcac ttcaaaaaca aaaaaagcct gggagtaaac
    11341 caccgaaagg atctctcaga acacaggttt tagacttttt aattgcaaga gccatcattg
    11401 atataacagc agatgaagaa tcgattcaag ccaatgagaa aaatgtagaa gccgaaatcg
    11461 atcggatgat gaaaggaagt ggtgtaaatg atcgagctca atttgaaaaa ttaatttcag
    11521 agagaactgg tttacctttt gatatttggc tcaatgactt gccctatcaa attaaaagag
    11581 ggcaactctt acaagttaga gttaccatta aacctccttc tgatcaagaa gtccaagcat
    11641 ggtacaatgc gaataaacca aaagttggtt ttgaaattaa attccgtgaa atttgtctta
    11701 cacctaaaaa tggctctgta gaagaagaac aacgcatctc aagtgaaatc aatagtatca
    11761 aaaaagaagc acgtaaagat agagatgcat tcacgctcat cgcaagtgga cctagaaacc
    11821 aagcagctgg aaaaggtatc aatgattggg ttcctacttt tgaaattttt aataagagtc
    11881 cttatttagt aaatgttctt ggacgcttag atgacggaaa gatttcggat gcatacattg
    11941 atgatcgtag acgttattgt attgttcgtt tggagggaaa gcgtccaacg cctctcgaaa
    12001 caattcgcag aaatatccaa gagattattc agagagaacg aattgactcc tcctttgatg
    12061 attggatttt aactagaaga aaagaagttt taattaccgt ttatgacaaa gaatacatag
    12121 cagaaaacaa actagaagct ccagatgaat cgtttaattt taataaaatt gatgcgggtt
    12181 caccatgaaa ccatttgatt tttctgtatc ttctgttgcg attttcctaa cgtcagaaca
    12241 aaaacatatc ttagaaatct cggattcttc tttttttata gatacttttt ttcgaaagtt
    12301 agatctactc tttccgaatc tacccatata ttctaatttc cgattttcaa atcgatccaa
    12361 tataattttg atcgaagtaa acacagaaag cgatttttta tttgcagtta ctgagaaatt
    12421 accagaatct atttcgggtg atgcagattt tgatgaaaca ttctttgctt attttatagg
    12481 aatttctcct ctgataagca ttgaattaac acagacgtta gtccaaagac atattcggta
    12541 tttagctcaa tactcctata gcgaaaatct acccaaagga attgttcctt tttttgtatc
    12601 tagagaattt gtatctactc ttccagagaa aattccggca actgtgcatg actatctagt
    12661 gaaaaattta aataattacg atgtagaaat attttttcaa gaaccagatc taagacaata
    12721 ccgactagat ttttctcttt ccgatcctag atccctaaaa acttccgatt tttttctaaa
    12781 aaagaatcca gacttagaat acaaagaact tcttactatg atcttagaaa atccaaatgg
    12841 atttagattg tttccttctt atatcgagat ggaaatatac agaggatgtg actataaatg
    12901 tagtttttgc cctcggcaat ttatttcgaa tgaaagagat ggagagctaa tgagtcttgt
    12961 gtttgtggaa aaatttttaa aagacttaga atcaagtttt ccttatcctc ttacattttg
    13021 tttcggtgga atgggagagc ctcttttgca tcctgatttt agttctattc tagcggctat
    13081 tttaaattat ccgcacacca aagaattgat tttagaaact gctctttatt caaatctaga
    13141 ttcactcaaa gaaggattaa agttagcagg ggaaaaccaa aagaaattaa atctaatcat
    13201 aaatttaaca acccttgatt ccaatcagta taaatctatt tatcaatcgg aattaaaccc
    13261 aaatgatatt ttggagaaaa tttctcatct atcgagtgta atgagtccta gtaatttaca
    13321 cgtccaaatg attaagatga aagaagtaga agaagaaatt gaaaattatt ttaatcattt
    13381 tgagaaaaag ggaataaatg taattttaca gaagtacaat cgttatgcag gactgatgcc
    13441 cgaaaagagg gtgagtgatc tgaccccaat tcatcgcgaa ttttgttggc atctaaatcg
    13501 agatgtttat atcaattcag atggtaatgt tgccatttgt agacaagact tcaatcatcc
    13561 aataggaaac ctaaacaccg agtcgatctt tacgatttgg gaaaaaggaa tgcgaatttt
    13621 tacagaatct cttaaaggaa atcacgacaa gacaatggct ccttgtctca attgtgatga
    13681 atggtatact ttcaatgcat gaattttttg cctttataca agctagaagc acatcgactc
    13741 gtttccctaa aaaggtctta aagacgattc cacccgaatc taatgacact attttgaatc
    13801 atattattca aagacttaaa aaagtaattc caagtgagaa tattgtatta cttgttccaa
    13861 agggtgacgt ggaagtcata gactatgctc aaaagaattc tattcaatac ttcgaaggat
    13921 cagaagacga tgtgcgagat cgttttattc aagcatctaa aaaatacaag gcagaatacg
    13981 ttatacgact aaccgcagat aatccattta ttgacttgga atatttagaa ttgcttatgg
    14041 aagctttcga agttcctgat ttagagattg cttcttttca gggacttccg attggaatgg
    14101 gtgttgagat ttttaaaatg agttctatcg agaaagaatt acctactgga ctagaaccaa
    14161 agcatcgaga gcatgtaagt ttacatttaa aagagtcagg cagttttcgt tttgaaaaat
    14221 ttcatacact tctatccaca aaagaaattg aaatttgtag taagattcgt cttacaattg
    14281 atgaagagtc ggattataaa ctctgcacag aggtttacaa taagttagct cctaaaaatc
    14341 ctttttttgg agtcaaggat gttattaagc tctatcaaaa agaaaaagaa ctttttcaaa
    14401 tcaatcaaaa tgtaaatcaa gttacattta aggtaactca caatgatcag aagaaaaata
    14461 gaatctttat tttatttgca gaacctaagg aatatggttc tggacattat gagaggtgta
    14521 agactttgtc cgttagtctt caaagttccg gctacaatgt atcttgtgcg tatgttcttc
    14581 cggaaaataa agactatgat ttatttatta tcgatcatag agatatggaa atcccagttg
    14641 agattcaaca taagacaatc attttaattg atcattttgg tcaggagagg tataattatt
    14701 ttccacatga tttacttccc catatgttta atgaatttga agacgttgtg cgtaactctt
    14761 tattcccaat tggcattgag tcttataagg aaattccaga aaagaaacaa gttttagttt
    14821 acgcgggaag tttgaacttt agatcttctt ttttacttga tcgttttgct tttaaacact
    14881 tcgcggatgc tggctatgaa atcattcgag taggtggagt tccaagaaaa aaagcaaatt
    14941 ttggaatcaa aacaattcct cgcacaaata aaagagactt tttacgaatg ctcgctgaat
    15001 ctgaattttt tatctcttat tatggtcaga gtgtgatgga cgcaagtttc ttaaataaaa
    15061 agataatgat ctacgctatc tccgattatc atgaaagact tgcacttcat ttttcaaaaa
    15121 actcagaagc agtttacgtt ggtaatattt atgtaaaaaa tatgaatatg acagctgcct
    15181 accaaaagtt cttacgaaaa gtagatttga atctacaaaa tgaaggactg acaatcttaa
    15241 aaaatacaat agcagattta ttaaaaaaga attcttaata tgaaacaatc tatactattt
    15301 ttattaattt tatccttatt cttttctgtt caagcagatc cttatgaaga ctatgaaaat
    15361 gaagatgaca aaactgtaac ggcagatcct gaccgtgaaa aaatgatctc ggatgcaaaa
    15421 gtcaaattgg aaatatctaa actaacgcat acagcccttg ggcatatcaa ggcaaaagag
    15481 tggaagaatg cggaagcagt cacaaagcaa atttttgaac tttcagaagt atcggtggat
    15541 tatttctatc taaaaggaaa tttaagctat tgttatggtg aatactatgc agccattggt
    15601 tttttaaata ctgcactcaa atacaatccg aaccatgagc caagtcattt tcttatggga
    15661 atgattcatt ctaagagaaa tgaatgggat aaagctattt attcttttga taaggcggct
    15721 cagtatggtt cttataatcc atattatcga atgaatcttg ccatttctta tttccaagtg
    15781 gataattttg ataaggcggc agcggaagca agaaagactc tggagttaaa ggaaaattat
    15841 ctatttgcga agattttgct aattaagtcc cttatgaaag taaataagaa agaagcttgg
    15901 ttttatacac aaggcttgat ggaaaaaaat tcagacaagc caaatgtata ttctctctat
    15961 attcaacttt tgtttgagta taaaaataat tatcctgaaa ttataaaaga gttaggcaaa
    16021 aaaaataatt taagcctaaa tgaaaagcgt tatttggcac attcctattt tagagaaaat
    16081 gatctaaata gatctttaaa tctttttaaa caaattattg cctccgaacg agaaacagaa
    16141 gacgaccaag tcatttatat gaaaatatta ttacaacaag gaaaagatgc agaggctgaa
    16201 agatatttaa ttagtatgat tcgatctaat ccttcacgca aaaagcattt taccgatatt
    16261 tatcaaggtc ttttagataa gcgcgacatg gttaagggaa tgtatctgcc gattatcgtt
    16321 cggtaatatt taaaagttct aaaagttttt ccgcactgta gcaaggagaa ccttgttttt
    16381 tctcctctgc ttctttgatt agattggcaa ttttattttg aatcggagca ccgatttgat
    16441 ttttctttgc tagagccaaa atctctccgt tgataaaatc gaattctact tttctttttt
    16501 tatttaaatc ttcccacata gaagatcttg cttctggatc aatttttatc atactcgatg
    16561 cgactctaaa aaataaaaag ttaggtagac ctaaaatgat cggagcgagc caaggaatca
    16621 ttttcccgag gcttactggt tttattttgg actctttcaa aatagaaaga gcttctttca
    16681 ttgtcctagc caatattttt ctaaacatag gatcggacaa ttcttctttg agtggaactc
    16741 ctgccagtgc gttgattgca ttgtttaagt taaaaattaa ttttccccag agaactcctt
    16801 ctagattcgg atgttctttt atttctagat cggctttttg aaagatagaa actatttctt
    16861 ttgaaatagt ggaagtttct tgaatcatta attttccaga ggttcccgaa tgaaagattc
    16921 cagatccttt agatataacg ttatacggaa ccattccagc aattactttt gattctttta
    16981 gaatactttc gattctagat gcattggtta ctccattttg gaagctaatc agtgttttgg
    17041 ttgaatcatc tttgaaaata ttttttaatt cttgtgcagt gctttctgaa tcctgacttt
    17101 tgactgttat taaaattaag tcacagtttt ttagttcttc tattgatgtt ttgtatttta
    17161 ttttacttgg ttctataaaa actttcttcc ctgaaaaatg ggaaagagtc attccatttt
    17221 ctaagatttc tttctgcagt ctttctcttc ctataaagac tacatcacat ccactataaa
    17281 gtagatgtcc accgatatag catcctatac ttcctgcgcc taatactcca atctttttga
    17341 tcattctatg gctccatagc tttttaacag ttcaatgact tttgtgtgat tcttttctgt
    17401 tgcatacata agagctgttt ttccatcaaa atcccgaatt ccaaaatctg caccagctcc
    17461 atttaaaata agtagcatta caatatcagg atttccaaac attgcagcaa acataagagg
    17521 agttcttcca aaagagtctc ttacattgac aaaggctccc atcttcaaag ctttttttgt
    17581 atgttctaga tgtccttttt ctgtgctgaa caataaataa tcatcagcgc ggctaatcgc
    17641 tgttaatgtc tgtgagaaaa ataaaacaag aatacagata aatattttca tacattccat
    17701 tcttctaaga ttcactttca gagtcatttc aaaaatagga atcaactgaa actggattta
    17761 ggattcttcg ctaaaatgaa tcctcgattg gattgtaagg gtattgtttt aatcttggtt
    17821 tatacttata tgcattgagc ctagcgtggt ttcctatata aaaataaaca ccaagcgctg
    17881 attctagaat tttatcctta caattagggc agaaaacatt tggagttgta taataggaat
    17941 tccaatggaa gttgtgacca cacttcgcac aagtaatctt tttataacta tcttcagcca
    18001 tttgaatttg ctttttttct gattcacttg catggttata ataacaagag agataataca
    18061 aagctaattc agacttgtct tctacgtata attcttttga ccctggagga aataacggta
    18121 aggcaagttt taaaatggca ggattgttct cttggattgg aaagtctttg attagaccta
    18181 aaattctatg cctatccgag tgagttagtt ctaagccttc aattatattt gctaaatcat
    18241 ttacccaacg ttcttttgaa aatgctatta ctagttcttg attttctttt actaaaacgc
    18301 atatcgcttc taaccaactg taaacatggg atgtatttct aaggaattct ttccattcac
    18361 tggaacttaa tttatattga gctagagttt taactgcttg tttggaacct ttcgctttac
    18421 tgatttcgat aaaatccttt cgcccattct ttaataaaat aagacttgca agtgtgacca
    18481 cagagtcatg ggaatcttct agccaaattc ttgctcgatt taagacatct gaatcgtttt
    18541 ctaaaaaaaa ctttgcaact ttttccaagt cttcttgctt caatttttct ccgtgtcttc
    18601 tcacacttgc ccagattgct tcgtatccgt ctttctcctt agataaaaga tctaacgctt
    18661 ttttttgaat tggttttctt ttactgaagg aaaggaatgc aattatatct agattcttag
    18721 aataaagcca gttttttaga attgaattat ttttataaag gagtaaaaac caatcctcta
    18781 accaattttt acaatagtag tggtttggtt tatttgcaat gactgcaaat cctaaccaaa
    18841 atgagggtgg gggaggactt gcatcttttg gattagattg agcaaggagt gattttacta
    18901 tttctaattc ttgtggaaca gaagctcgtc ttaatgacag atacgcttca ctttccgcct
    18961 gacgccaatt ttctagtttt aaaatatctt gatttaaaac tctttgccat tcttctttta
    19021 tcatcttctc gattcttaat ccataggttg tccaccttgc atagatagaa aaacgaattc
    19081 tatcttctct taaatttgat gtgagaagtg gaagaacctg ttcttttatt agaattgcaa
    19141 attgttcttc tccccactcg acccatgttt ttgagagatt ttctttttga ttaacagtga
    19201 tttgccactg attttctttt tccattttat aagcagagta aacattgccc tctttatctt
    19261 ctgccaatgg atataaatct aaccttttaa attctctttg tttttctagg cattggatta
    19321 ggctatacca ataaagccct gttttccaaa aaggatttgg tcttatttga ccatttgcct
    19381 ctttgaacca gtagattact tcttttgcga gagaaacctt atattctttt tcaattgttt
    19441 caatttctaa ttcatttaaa cccttctgga ttgagttttt ttgctctggt ataaattcgt
    19501 ttgtaatgta gtttatttct ttccagataa atgtgaattg atacatgctt taagtccagt
    19561 gtttagaata ggtctatttt ttttactttt caagtccacc ggtttattaa aaaaaaataa
    19621 ctatccttgt cacaaatggt tttagagtct tgtcctacta ggtattaaaa aggagtttca
    19681 aatgaaatta gcggcaaata tatttacagg gattgtgatg ttagaacatg tttatatcct
    19741 agttctagag atgtttttat ggaatacacc aatcggaatg aaagcattcg gctttacaca
    19801 agagtttgcg gacatgacaa aggtgctcgg tgcaaatcag ggtttataca atgggttttt
    19861 ggcagcaggt ctgttgtggg gatttttaac caaggatcta gatcagagtc actcgattaa
    19921 acttttcttt gtaagttgtg tagcggttgc tgcaatctat ggcgggtata gtgcaaatat
    19981 ttccatttta tttaaacagg gagttccggc gtttatcgca ttgactctac tcttaatctc
    20041 cggaaggaag taatttattt tttatgccta aggcaataga tgaaaataaa atccaccagt
    20101 tactagaaaa agcagtaaag ggaaaccaac ctgcactgga atctatttta aaggaagtgc
    20161 aggattttat ttataaactt tctcttcgaa tgctttggaa ccctgtggat gcggaagatg
    20221 caacacagga aatcctaatt cgaattttac agaatctaaa aacgtttaaa tatgagagta
    20281 aattttctac ttgggtatac cgagtgacgg taaattttct aatcaatcaa aaaaagaaaa
    20341 cgtatgcact cgaggaaaat tacaaaggta tgattcgaaa attagaaaaa gcaaattcac
    20401 aaattgaact agaaaaaaaa caggatacag aaggtttaaa gcttggatgc acctatgcaa
    20461 tgcttttatc attggatcga aattatcgca tcgcgtttat actacactac gtttttgaac
    20521 taaagggaga agaggctgca gagatattac aaatttccga agtaaattat agaaaaagag
    20581 tctcccgtgc taaagaaaaa ttactctctt ttatgactag ccggtgcaat tttatcaatg
    20641 attcattaac ttgtaaatgt cctaacgttc tcactcattt agtatcgcaa aattttatca
    20701 cttcacagga tactaaaaaa ttaaaatcca catatgggag taaaattaca gataaggaaa
    20761 ttcttcgaaa ctatgattcg atggaaagac ttgctatact ctacaaagaa aactcgaact
    20821 ggagatcttc ttctatttta aatcgaaaat taaaaaagct agttttagca gaatagctat
    20881 tttccccagt cgactctccg aatgattcca caatttccaa aactacaaag cccagaattg
    20941 atatctggat ttccttggtt tgttttattg attgcattca attgaattgg tgcaaatcca
    21001 tggactaatt ctacaacgga tccatttgga ttttcagtag acatttcaat ttcaatcaaa
    21061 taaaaatttg cagtgtcttg aatttctctt aggaaaatct tttcagtcac tgtgaatttt
    21121 tcgattaatt cttttgagga gtaatctttt agatcaaaga gttcataaac agaaagtcga
    21181 tagcctttgt ttttatcatc tgaagcagcg cctattcctg tcgaaaacat acgcccctgt
    21241 ttaactaaaa taaattgaac tgtctgttta tttaaaagtt gaaaatgata cttctttggt
    21301 tggagtaaat atttcctttt ttgcatttct ttttttacga tcaatacatt ttcatttaaa
    21361 gtgctttctt gctcttgggc gttgacttca aacagaaata taaagagaaa tgaaattagt
    21421 atttgtagga tcatggtttt acagtctcgc cgttagactc tataagtgtt ttaatttgaa
    21481 tagaaatttc atagagctct tttactttct caatcgggaa agattcttta tgagaatgat
    21541 ttgctatctt tgccgcttgt tctaaatggg atgaaattga ttttaaaagt tttttctctt
    21601 tttctgtttt tgcatgatga atcagagctt ccattatatc taaatagatt tgcgcaatat
    21661 gaggctgtct tagaatttct gcatacatag gtttataatt attaataatc cctttgctcg
    21721 ctatatgaag tccttctagc catgctccta gttccataag aactgctaaa tgatctttct
    21781 ttttatcgtg taacgctttt tctatttcat attctaaatg aataattctt tcatctaaaa
    21841 cggattggtc ggcatgaaca ataagtgctg tatccaaatc aaagagtcta tcttttattt
    21901 gttctgttat ctctagtgaa tcagcaagtt ttaaaaagat tcctcgtgct tcaattgctt
    21961 ttattttttc atctgcgaag actaaaaccg ttccatcggc agatctacca cctagattta
    22021 aagctctctg tgtttcattg tcgtagtcat gagtcgggtt actttctgtt agcccttgcc
    22081 atgggaaacc catcgaattt aaaaaatgaa aggagtgaac aacagatggc atgatataat
    22141 ttttgccatc aatggtaaaa ttgaattgca catcttcttc tggtggtctc acctttagct
    22201 tttggttgta gtaaaaaaat cctacaagtc caaggctaaa tataagaact aacgttaata
    22261 taatatattt atactggaaa acagtttctt ttaaatttct tttaaatttt atcataatta
    22321 cctcacgcaa gtgcgaccat aatctattgc agagcaagga attctaccgg tgtctgattt
    22381 tcgataacca gcttgttttg ctcgaattgg atcgctataa tattcaattt ctcctgtatg
    22441 ttcgtagtaa aagtctttta catcttcttt tgttgcaagc tgcataggcg cataggcaac
    22501 taaaacatca acagaggaat aaccttggtc tagttttgta tctaggagtg tgacttcgac
    22561 tatgtaatga taggacttat ctgtgatttc ttttatataa ttggaagatt ctatatcctc
    22621 tccttcgatt gcacgttcct ttccttcaga tgaaatttgg gaaaaaatta ccactctgac
    22681 tttatttaca ttttcgtctg tcgcaatacc gatggctgtt ttggatagga agactggcac
    22741 tgtaatcaca tatcggacgg attcattttt ctttagacca atgtgaaatc ttcttccacc
    22801 ttcaattgta taattctttt cttttaatcg gtttaccact ttactcacat ttgcacttaa
    22861 acttcttcct ggaagaattg ccattgagca aataggacta aggacgatta atagaatggc
    22921 aatagattta ttcatatatg gttcaatcat ttgaaaggct tagttttttt cgtctctttt
    22981 ttttgagaag gaaggaataa atatgataga ctctagcatt taagattctg caaagaagtt
    23041 gaattttaga aatgtcatat aggattaaat atctaaaatc tttttggttt aaaaaatact
    23101 ttgcgaatca aattaagtat gtatacttgt agaaataaaa actggctcaa agcagatatg
    23161 gggaattttc attccttttt agatgaataa agttttcaga gataagcctt ttttataatt
    23221 ttaggagata ggataagatg ctaggctttg gtatttttaa aaaaatacag attagttttg
    23281 ctttaaatag aatagattta gattacaaga gttttattct cttctctcta tttttaggct
    23341 tctgtaatcc agctgaattt gaaaatccat gtgatcctaa atcagaaacc tttcgaaaaa
    23401 attatttaat aatatcagac ttaggaataa agagcggtta ctgtggaata tcctcaactc
    23461 cgttttctaa tggatcgaat tccaatacag gcacaaatca aactcctacc attcctacta
    23521 aggttgcgaa atttttaatg gtatcaaatg ctggatctaa caatgtgaat gtatatacga
    23581 ttaattctac aactggagcg cttacccaaa tttctggttc tccctttgct gcaggaggtg
    23641 gagctagatt tgtaactgct catccgagtg gcttatttgc atatgttgca aatggttctg
    23701 gttctagtat atctgcttat ttaatcaata cgacgactgg ttttttaact ccgatttctg
    23761 gttctccgtt tgctatacct gttaacccct atgcactcac gattaatgcg gcaggcactt
    23821 tactctatgt agctccctat tctggaacat taatttatat gtattctatc aatcttacaa
    23881 caggtgcttt aacttttgtg aattcatttt caaatacggg tgtttatgct gcatcccttg
    23941 caatcgatcc aacaggtaaa tacatgtacg ctggtgatac aacgaatccc ggtcggattg
    24001 atgcgtttca aatcgatagt tccaatggct ttctaactcc tatagccggt tcaccgtata
    24061 tcaatggagg caatgataca ataggcgtaa cagttgaccc gacaggaaaa tatgtttatg
    24121 ctacgaatta ttttacaaat gatatttatg gttatgtttt aaatcctacc actgggcagt
    24181 tatcgggtat tccaggttct ccctttgcga ctgggagttc tgccccaacg tttataattg
    24241 tagaggcaac cggtaaattc ttatatgttc caaacagtgg cacaaataat atttctggat
    24301 tcactgttaa tcaaacaaca ggagcattga cttctatttc aggatctccc ttcctatgtg
    24361 gggcatctcc gagcgttgtt tatgcagatc cttcgagcac ctttctttat tctgcaaata
    24421 catcaggaaa taacgtttct ggatttacga ttaattctac aacaggtgcg ctgactccta
    24481 tagcaggctc accttttgcg gcaggaactt ctccatttgg ttttggtttt gtaagttatt
    24541 aattctatta tttaaatttt aaggagtaac tgatgttacg aattttactg attggcttat
    24601 tcgtattagc cgttaacacg tttgcagaag attctgctaa aaaaagctgg actcaaaaaa
    24661 agagagtagc actgatcgtt ggagtcaatg aatacaaaga tacgacctct ttaaaatatc
    24721 cagtcagtga ttccaaaaag atgaaaacta tttttgagaa agttggctac tttgatcata
    24781 tcgaaatatt aaatgatgag cagggaaaag cagataatac aaagttgcct acaaaagaaa
    24841 atattgaaaa tacttttaaa gcactcattg ccctaaagcc agatacgatt ttgttttatt
    24901 tctcgggtca tggtttaatt gatggagaaa aaaacaatgc catcgctcct tctgatttaa
    24961 aagtttctaa ggattttaag aagatagaaa atacaatcaa tctagatgaa cttgcaaaac
    25021 aagttgatac atcgattgaa cagggaattt ttttaataga tgcttgcaga agcccgcttc
    25081 ccaaaggtga aagaatttct ttggataaag caaatgtcag tgaaacaaat ttaaaagaaa
    25141 aacaaatctt aaaaccattt tcctggaaaa agaaactaac gttttcaacg gagacgataa
    25201 gagaagatgt agaaaatgcg cggggtgtta cagttttaat tggaacttcg cctgacaatt
    25261 cttccttaga agatgatagt ataaaaagcg gaatctttac gtattatatg atcaaaggtc
    25321 tagaaggtgg gattttagat gagaatatag aagaatatgt tacgatgtct tcccttcgtt
    25381 cttatataga taaaaaattc atcgcacata aacaagaaaa tctacaaatt ttagaagaga
    25441 atttaaaaaa aacaaaaaag gattcggaga actatcaaat tttacaagaa aaagtgctag
    25501 agactaagaa gagtttgccg cagaaaactt atttaattgc tccagctaga ggtgtgactg
    25561 gagatgtaat gatttctttt ggtagaccaa acctagacaa tctaattgag agagtaaagt
    25621 attttgatga tgatggaatt ttgattcacg cgaaggttca tttggaaaac ggaagtagaa
    25681 agtcctacat gaattttttt tcaaaacagg agtccacggg gaagttttat ccagatgatt
    25741 tagatggtgt aggtaaaatg aaatttacct atgatgaaaa ttatttcgaa gcaagacagt
    25801 ttgataaaga agggagagaa acttttactc ataagctaga atatgatcct gataaaaaaa
    25861 attggatcta tgcgggagtt gtttccaaat cagacattgg agcaaagttt gaaaaagtaa
    25921 cgtttgatac aaaaggaaaa attttaaaga agggctattt cgattggaat agcgaaaatg
    25981 gatttatttc ttatatagga ggaataacgt atacatatga ttctttggga aaaataaaag
    26041 aagagttaaa tgctgttaaa ggaagggatt atacagtaaa atatttttat aacagcaaag
    26101 gattgctgat ttctaaaaaa atataccgat ttgataatgc aaagaataaa gacattctat
    26161 acgaggatga aaataatacc catgaataca aatattctta cgattcatcg ggaaacttaa
    26221 ttttggaaga atattttaat ttaaaaggga aactacaaga tgatctaaat aatatcgctt
    26281 ataaaaaata ttcttacgat aaatctggaa ataaaataga agaagaagca tttcgttcaa
    26341 cgcacaatcc acttagtaaa gaaggagtta caagcacagt ttttaagtat gattctaaaa
    26401 aaagagtgat tgaaaaatct tttttcgcag aagcagaagt tgattcaaat aaaaagaaaa
    26461 tcaaacagat agatgatttt ggagtccatg cctatcgtta tacctatgat gaggaatgcc
    26521 ttcgagatcg aagcattgct attcgtagaa gaagagattt ttaccaagaa ggagtggatt
    26581 ggcaggcaaa tgccaagaca tctgaaaact gttataaatc tgttgaaatg ctagatcgat
    26641 ctggaagtcc cacaccaaat cgtttaggtg ctaccaaaat tgaatttata aatgatggag
    26701 gaagggaatt atttaaacgc tatctaaata gtagcggcaa attcttgtat gccgacattc
    26761 cacaccaagt ctattatgca aaccgatttg atgataaagg aaatcatatt gaaagagatc
    26821 tacgacttgt atcaggagaa cttttagaga atcaagatgg ttttgctgga attcatttta
    26881 aatacgatca agatagacga gaaatcgaga gaatttatat cggtgccaat ggaaaaccag
    26941 tcacaggaat cagaggagtt gctaggtacg taaatacata caataaaaat ggaaaattga
    27001 tttctgtaga aatgtttgat cccaaaggaa agttagtcgc cgaggatgaa tcaaaagtag
    27061 caaaagaaat ctataaatac aatgagaaag atcaactggt actgaccgaa tattatggag
    27121 tggatgggaa attgttttct gacggtggac ttgctcggga aaaaagagat tacgatccaa
    27181 atggaaatgt aattttaata gaagaatatt cctcagcaaa taaacttatt acaaaagaaa
    27241 aaaatcaata caatccaaag aatcaaatca ctcgaaaaga aatttggaat gaaaaagaat
    27301 tggttttaaa caaagagtat ttttatgaca aagaaaatcg tttaattgaa gaaaatagcg
    27361 gaagtgaaaa aacgaaatat acatatctag atccaactca ttttaaagaa gaaaaagaat
    27421 taaatgggca aaaagtccca gtattcggaa atgcattttc cgtcctttat gaattaaaca
    27481 gttctgggaa aattatttct aaaacttatt tagatgagtc agggaaaaat cctatcaatc
    27541 aaaaaaggat gccttccgta tatcgttatg aatatgatgg cgatggaaat ttaatattag
    27601 aagagtgttt agatttttat ggaaatctag ttgatccaaa tagtaattta gaacctgcac
    27661 gaaaaatttt tagatatgat gcaaatagaa ataaaatctt tgaaacatat tttagagaag
    27721 atgggtggct atttaatatt atccgcaatg gattgagtta ttccagacat acaatagaat
    27781 acgatttaga atgtgcaaga gattttatta atagaaataa aagtaaaaat caatctgaaa
    27841 tagaagttat gctcagagag ttttcaggat gcgaaatctt tagatcctat cacaaaagta
    27901 atggggagtt attgcaaaga tatactagag acaaacaaaa caatgaatat ctatcaatgg
    27961 aaaaagttga tgcaaaagga tatatcagtg tagggggaga aagaggaatt caaaaagata
    28021 cttatatcca actctttgaa aacccggagg gaaattccaa ttcaaagcga atcaggctta
    28081 cactcgatcg ctttcatcgt ccgattggaa tttccttcga agcagggaaa cggtagaaat
    28141 ccattgtagg gattgtgtaa aagcaagatt tttaccacga agtgtacgaa ggccacgaag
    28201 aaaaatggta aatttcttag gaaattttga ccttcgcgat cttcgtgctc ttcgtggtta
    28261 gatttttctt taaactttta tacagttcct actatggctc gtttacaagc ggctatttat
    28321 tgttaagcgc tgcaactcct ggtaggactt ttccttctac tagttccata gaagccccac
    28381 cacctgtaga gatgtgcgac attttatcgg aaacgcctgc tttgtttacg gcagaaaccg
    28441 aatcaccacc acctattaca gtgatcgcat ttgagagaga tgcaagtgaa tttgcaattt
    28501 ctactgttcc tttcgcataa ctttccattt caaagactcc aacaggtcca ttccagaaaa
    28561 ctgtctttgc attggaaaga gcagatttaa aaagatcaat tgatttcggt ccaatatcga
    28621 gtcccatcat atcagcagga atttgatcga tatcgactgt ctttttattt gcatcatttg
    28681 caaaagcgct cgcaacgatt gcatctgttt gaaggataaa ttcaatattc ttttcttttg
    28741 cttttgccat tgtatgcttt gctacttcta gttgatcgag ctcaacaaga gaagttccca
    28801 cttcttttcc cattgccttg aaaaatgtaa aagccattcc accaccgatg atgattttat
    28861 ctactttgtc cattagcttt tcaatcactg tgattttaga agaaaccttt gctcccccaa
    28921 cgattgcaac aaaaggctta gctggatttt ttactacttt ctcttctaga aattctaatt
    28981 ctttttccat taaaaatccg gcaactgcag gaagatattt tgcaagtgtc tcagtcgatg
    29041 catgtgctct atgggcggtt ccaaatgcat catttacata aatatcaccg tatttagcta
    29101 attcttttgc taatttttct ctgtcttctg ctgattcaga agtttcttct ttgtggaaac
    29161 gagtgttttc taaaagaacg acttgtccat tggagagagc atttactgtg gataatacag
    29221 attcgccgat acaactatct gttactttta cttctcttcc taaaagtttt gcaagatgtg
    29281 ctccgatagg tgccatctta tgttgcccat tgatgtaatc ttcttcatta aaaggctttc
    29341 ccgctttttc agctttttct tttgcctttt tcatatcctt tttgggatca cctagatgag
    29401 acattaatac taaggacttt gctcctttgt ctagaatgta tttgatagaa gggagtgcag
    29461 cagtgattct agtatcatct tgaatcgctc catttttgat tggaacattg aagtcgactc
    29521 gcattacaac tcttttgcca tttagttcca tgtctctgat tgtttttttt gaaattccca
    29581 taagttcctc ttttgtttaa ttttgtggtt aattctaatt agccgtttct ttttcccaaa
    29641 gtctcatctt tataaaaaag ataacagata ctaaaataaa tgcgatcgat atatattgag
    29701 attgggaaaa tccatgccag taatatccag ttaagaaggt tggatttcca cttgcatctg
    29761 gaatgtttac catctttggc ggttcgataa aagggaagac cgctttgttt acacgtaaga
    29821 attctataca taaacgggcg aggccatgga gtgcaatgta ttgaaaaaag atactccact
    29881 ttttgaaatt ttgaaaacgt gcccagtaat gaaaataaag aaaataaaga aaagagatga
    29941 ttgcttctat cacgggagta ttccaaacag gaactccaga aggatgtgct ccatggaaat
    30001 taaaaactag aatcggaatg tttacatctg tgtgaaatcc ataacacccg tcaccggaaa
    30061 caaagcaacc gagtctacct atcgcatatc cgatcgccat tgtagaagcc attccatcgt
    30121 agtattttcc taggtcgtat tcattccatt ttaaatagaa atagataaaa agagttccaa
    30181 ataaaaaacc accgtaaaaa acaagaccac caccattaaa aagagaactc caaagaccca
    30241 tactcgttct acatacttca caacttgggt catttaattc ttgaaagccg ttccaatgtg
    30301 tccagggata agaaaacatt ccgggaacca caaagatttg atcccaaatt tcaaagatgt
    30361 aaaatatttt tgctccaacg agagtgccaa gaactcctaa aaaaacgatt gtgtcagctg
    30421 cttcaggaac gagtcctttt cttttgtatt cgagggggag aagtttagat ccagttaaaa
    30481 atgcgatcag cattaaaatg gagaaggtag aaggacctcc ccagattcct tctaaaaacg
    30541 gaattgaaat tctatctatc atattataac cagagaagtt ggttttattt aagaggcaag
    30601 atgaaaaact aatcgatgtt tataaattct tgtgggttga ttggcggatc gagtccaatg
    30661 tgtacttcat agtgtaagtg agatccggtt gcttttccag tggatccaac gagtgcaatg
    30721 atatcgcctt gctttacaac gtctccttct gctacgagta atttggaaca gtgaccatac
    30781 atggtaaaaa atccattttc atggttgatt cgaacatgaa gaccaagacc tccgtccgag
    30841 ccagaagcat ccgctacgat tccaggcgct gttgcataga ttggggttcc atttgcagaa
    30901 gcaaaatcaa ttccattgtg aaactcacca acatccacat aaaacggatc ttccctatgt
    30961 ccataggtgg atgtaatcag tccaactccc ggaccaagag gtctacctct cggcatagat
    31021 tcaaaaatac tttctcttgt ctctaaatag tccattgagt tttgaaagtt tgctttgagt
    31081 gtagataaac tctcgtatac atttgcaaat ttttctacag tgtagataaa aattttcata
    31141 ttagagtcta gctctgtttc cggattgata tttgcttttt ccgccgtttc ttgcatcaaa
    31201 cccaattctt cttttgcttt agagtctctc aaagatctct cccaggtcac cttatttaaa
    31261 tcttctgttt cagatgctag gtcctcaatt gtgtcttcta gtatgtctcc cattaggtca
    31321 tggtgtaaaa agaaaacctt ttctgtattt ccacgggaat atagatccac atgatcattt
    31381 cgagtataga tatagtagcc aagaaaggaa attcccatga taaaaagact ggttataagc
    31441 aaagctagaa atccaaggga atagtgggaa atttccactt tgacaatatt ttcattgcta
    31501 tgaggaatca gcagaaggga aactttggtt ttaccaaaaa tatgtagctt ttggaaactt
    31561 ttttgtaatc tgtaatactt gagagtcatt ctctcaagca aagttagctt attttccgat
    31621 aattccatta gagcgttaat ctattaaagt tccaagtttt agctacccag tcttcgcgtc
    31681 aatttgtagt tgattttaat agataaaatc ctagcttaga caatatacac atgataaaat
    31741 ttatagcaaa tctttttaaa aaaaagccac agactgttga caatattcta atctaccctg
    31801 aaggcaaacg ctattacaaa gactcccatg tgattcgaaa aaatttaatc gatgaagatg
    31861 cacttaaaat tatgcataga ttgagcaagt tcaatcataa agctttttta gtgggaggtg
    31921 gggtaagaga tattttactc gggaagaaac caaaagattt tgatatagcg acgagtgcta
    31981 ctccaaatca aatcaaaaat attttcaata attgccgtat cattggcaaa cgattcaaaa
    32041 tagttcacgt tatgttcaaa ggcaaaatta ttgaagtctc aacatttcgt tccctacctg
    32101 atcatagact tgggaaacaa acagaggaca aggattatct cctaagaaga gataataatt
    32161 atgggacagc aaaagaagat gccgcaagac gtgattttac aatcaactct ctttactatg
    32221 atccaagaaa tgattctatc atagactttg tgggtggatt tgatgatatc aaaaataaag
    32281 ttcttcgagt cattggagat cctgacgttt ccttcaaaga ggatccggtt agaatgctcc
    32341 gtgctgttaa gtttcaggtt ctacatggac taacgttaga aaaagaaaca aaagcgggaa
    32401 tcaaaaaaaa tcgactagaa ttagagaaag catcttcttc tagaatgtta gaagaataca
    32461 ataaaatctt tcgcacatgg agaacttctg aaatctttca aggactagca gaaaattttc
    32521 tattagatgt tttgtttaaa gatgcatttg atattgcaaa aaagaattcg aattggcaaa
    32581 aagaattttt agagacagac attggaaaac gtttagtgat tgcagatcga atgttgcaag
    32641 agcgagagga actaacgtct atcattttct ttgcacttat cttttctgat ttagtaaaag
    32701 acgctttgga taaagacaag aacaagcaga atatggttca tgccataaaa gtagccctag
    32761 atccaatctg tgctagaatg gaaattccaa agaaagacaa agagagattg ataaaaatct
    32821 ttgcgagcca aaaaagattt ttcaagacgg aagaaacaaa tccaagtcac aatgaagtat
    32881 tccgtaagaa agactttttc tatgaagctt ttatggttta taaaatcaat gcgttagcag
    32941 agaaacacga acaagcaatt caatcggctt ttttctggga aatttcaaca cgaaatcgtc
    33001 caaaaccatc tcaaagaatc atccacaacg aatccgcagg tggtgaaaga gaaagattta
    33061 gaaactttag aaatgaccga aagcccaagc gttttaacaa acagaatagt tattcaaaag
    33121 atcacgtaaa agaaagtcat tcgaaagaga atcaccataa agatctccat ccaaaggctg
    33181 tttcggaaga agaagtagta aagaatcatc atgctgagcc ccaagaattg gatagaaatg
    33241 ttcctgagga aattagagaa gtagaaatcg aggaaacagt tgtagtagag tcgtctacag
    33301 aggctaataa tacgaatccg caaccacaaa aagaaagaaa accattcaga agaaatcgat
    33361 atagaaatcg ttttagaaga tatggaaata aaaacaaaaa aacacaaggg gagaattctc
    33421 ctaacaacaa ttcacaagaa aaacctgaat aacaatgagc caagatagac attctttcaa
    33481 aagtctactt ggctttgttt taatagccta tcttttttta gtatccttct gtcttttggc
    33541 agaagaaaaa atagactcta tgattttgca tgaacctaaa gatgagacgc aatacgggtc
    33601 aaatattcgg cgtgcagaac agactttgaa tcaaacgatt tcaaaattat atctagaaat
    33661 ttcttcttat tctaatctct taaatttaaa aattaaatca cttccccatc ggactcttgt
    33721 gaaaaaggga acagcaaaag gggaagattg tattatttct actacagaaa aagatgtaga
    33781 ttgtatcagc attgagcaat ttgattttat agaaggttca tttggagaag cgaaaggaac
    33841 aaaaagtaaa ttgattgttt tatttttcga tcaaaaagca tctgcggata acaaagattt
    33901 gtctccaaaa ctaaaaaaag taaagagtag aatctattcc cataattatt attcccaaga
    33961 taaaacatgg atggaagtgg ttgacaatga tccgttagga aatccagatc atgatgataa
    34021 aattatcatt cgttctgaat ccaatcctta tttttcttct acaccagtca agaccgagga
    34081 agttggaaat cgagaaagtt attctctaaa gtctattgaa aatacaaaat cccatccaat
    34141 tcgcaatcat ttcaaacgag aagcctattc caagcatctt cagctttttg aaaaattatt
    34201 agctcgaatc ttaaatttta atttgaagac agaaaataac aagaactgaa ttttataaaa
    34261 aaagtttctt agtttcctca tccactggaa ataatagttc taacctgagt tcttgggaag
    34321 taacgtctgt gggagttcct aaggtagata gaattgtgaa aaagctagcc ttcttatttt
    34381 ctttttggag tgtaaatttc agaacgggta aattcatttc ttcctgcgtt gaaaatactt
    34441 ctttggctct tgggtgtctc gagattttat gatacagatt aaatagtttt ttgctttggg
    34501 aaaaattcat ttcctctttc aatcgattca aaataaaact ctctagttgt gatagattct
    34561 gtagataatt tcgaaatcca tctggtgata gaaacaattc gattgaattc tcaaattgat
    34621 gaaatgcatt tatgccagcg aaagttttta caatttgttc atatccttta tttttcatta
    34681 atatttgata actagtatcg acaacgagtg cagggtagcg ttcatgttta tcaagtatct
    34741 gcagaaaagc atctcgaatg atttttaact tgtctgaata gaatgactct tcactgtatt
    34801 caggtgaata accagctgag attaaaaaaa gattcttttg gcgaaaaggc attttaaaat
    34861 aatccgcaat tttcaaaacg agagatctac ttgggtttga tttaccagtt tctaaaaaac
    34921 ttaaatgctt agaggaaatt tctaaatcta gagaaaggct taattgactt attcgttttt
    34981 gttttctcca aaagcgaagc aattttcgaa aggaatcttt tttaattttt gtgggcattt
    35041 ttacctctga ggtaattgat accaatctct tatgtgctat actttttcaa agtaaagaaa
    35101 tttttaaagg acaaggttta tgcaaatcac tcaaatttta ccatatctag caggaggttt
    35161 tttagccatt tatctaattt ttacaatcat ttcgaaagag aaaagtaaga taggttggat
    35221 ttttccggca tttctatctc ttcttttttt tagctattct gtatttactg tatcacaaga
    35281 aggtcccata ggtttttggg tagagcatac tcgaaacttt tggggaaatc aaatttggtt
    35341 tgatctatta cttgccgcct ctattggttg gtattttatt ttaccaaaag caagagaact
    35401 taaaatgaat ctaccctttt gggcattcgc cattgttgcg actggttcta ttggatttac
    35461 cgcaatggta tcgcgcctat tgtatctaga agagaaaaga gagaagcagt aagagaattg
    35521 aagtaaactt tccttgctta gaactaaatg agagaggata gatgcgaata ctcttacttg
    35581 aaaaaacttt cttaaaagta agagtattta aattgtgacc atattaaaat atatcatcag
    35641 ctgggatttc atcgatatcc gatgggaatg gatctggatg tgaagtcatt ttatttgtat
    35701 cagtagaaga gctagctcct gagtccgatc ttccacctag aagttggaaa ttttcgactc
    35761 taatttttac cttactcatt ttctttccat ctggtgcatc ccaagtagat tgttcgagtc
    35821 taccttctac catcagttgc tttcctttcc cgccgtattg tttcaagact tcggctaact
    35881 ttccccaaac agtacactca aagtagttta ctaaatcctt tcgagttcct gtattatcta
    35941 catacgttcg gttgtttgcg atgctaaaat ttgcaaggga tgtttgtcct accgttttat
    36001 attctgcatc tttggtcatt ctaccaatta aaaccacatt gtttaaatca tttgccataa
    36061 attcctcact taaaaataaa taattacaat tactaacaat ctaaagccat taagttaaat
    36121 atttaaagac taccaccgat agggacgatg aacaccgatg aaaaatccga ttatataaaa
    36181 gtaatcatcg gttttatcgt tttgttatcg gtgtcctcgg tggttattcc cttttcttat
    36241 ataacattgg ttatctgccg tatgaattta ttttgtttct aaaataagat tataacaaca
    36301 tacggacaag ataggatcag attagaaata ctttgctttt agaaataaaa aaaagtcaat
    36361 ctgtatatgt gacttaattc tgaatttaca aagcaatttt atgtgcataa actgatttgt
    36421 atgaagataa ttgcaatttc tctctacatt ctcagtggat tattgttttt ttattccctg
    36481 acacaaccag tttttctatt ttcagatcat gaacctatcg agggcttgca tgttttattt
    36541 tggggttggt ggggaatgat tttaggacat ccaggctggc tagccaattg gctttattca
    36601 gccacgatta caatgaattt acttggtaag cgcaaaattg ctattatcct cggcctcatt
    36661 gcggtcgctt tttctttgga tggatttaga gttacagaat ggtactttaa tgaaggaaat
    36721 tctactccta ttaaagatct aggagtaggg tattcctatt ggcttgcggc gattggggtc
    36781 ggtgcttttt cgactctttt ttctacaaat cagtttatcg attaattttt tctttgtaag
    36841 aatcattaaa gcctttccct tctagaatca gaggtctgta ttttttaatt ctatctattc
    36901 ttgttttgga ttgcttgggt tgggaaaaat agatgagata tcccttttgt cttcctggtg
    36961 ttaatgcttg gaatgccttt ttaaaaaccg aatctttttt aaaaatcgat tccaactcct
    37021 ctggaacgtt atactccgat acatcctttg tttttacttt ttttcctgat tcttctattt
    37081 taatagcctc ttggatgtat tctttcagag aagattcaag tgtttttaca tcttttaaat
    37141 tggtaaatcg aatgagtctt cctgattgac tgttatctcc ggctgtttct aaaatcattt
    37201 ttggatcttt tagaagagat cctttaaaaa aattaacaga gcaatattct ttaaatgctg
    37261 ctagaatgag aatattcttt ttgttgtgag tataacaggg catactccac tttatttctt
    37321 ctactaattc tgtttctaat aaaatttttc tcagggcttt taattcctgt tcccaattgt
    37381 gaactttaca atctggtgta tcataaagtt tacatcttcc acaacctctc actagatact
    37441 catttacttt tatatttgct tcgttcattt acacacacct tttatattcg taaaatagaa
    37501 tcccaaaatt tcatagagct attttacaat ccaatcaaaa ataaaagttt gaatcaatta
    37561 tttaatattg attccatttg atgaaaaaat cattcttggt ttacatggtt attataggga
    37621 tttatagaat gaaagatgta gaaaaactaa aaatagattc agaattcggt gctcacactt
    37681 attctttaaa ctacgcaaga gtgcagcttt ttaataaggt attttttatt tgtaatctca
    37741 ttgttatttt gctgaatatt gtaaattatt ccaagggaat tggaaataaa aacccttatt
    37801 cccaattgta tattcctgct tatttttttt atttagtcta tttttttatt atgagtctaa
    37861 tcacgtttta cctcaagtgg caacctgcgg ataagataag atggtttcac aaagtaaatg
    37921 tttatttatt cgcagctatg atgttagtat ggatcaatgt gattactttt atagacctgc
    37981 gagtaaatgg aggaatcaca gtttatgccc tcggcttatt aggcatggct tccatactat
    38041 ttttaaatcc aattcgagcg gttatttttt atataatcag tgcaattgct ttttatactc
    38101 ttttaaaaac acataatatg ccggaagaca agatcattgc agattcagta aacgggacaa
    38161 ttatgatagt ggttagcttt attatttctc aaatcatttt tatggaaaag aaaaaacaat
    38221 ttataaaaga tattcaaatt caaaaacagc aatctgattt ggagaaaata aattcagatt
    38281 tacagtcaca gaatttaaaa aatgaaactc tccaaatgat tattaaaaat tatattcctt
    38341 ggactacatg gaaaaaagca gatttaaatt cgactctttc tcttcctaag atcccagaag
    38401 aagaaatcga aataacttgc ttatttttag atatagtaaa cttcactgtt ttttcggaaa
    38461 agaattctcc aaaggttgtg attgaaaaat taaataaagt ttttagaccg atagtcgcta
    38521 ccatttatcg aaataatggt gatatagata aatttatagg tgatgcagta tttgccgttt
    38581 ttgaggatcc aaagcaagcc ttgaagagtg gatttgaaat ttttcatctt ttgagagaag
    38641 ctgatttaga atctatggac gatatacgaa ttgggattca tacaggtaga gttattcgag
    38701 gggatgtagg aggggaattt agaagagaca atactttgat aggggatact gtgaatattg
    38761 cttctagatt gcagacatct tgccttccaa atgaattaat tatttcggag acttcagcca
    38821 atctttgtca aaatcagtct gttgcgaaat aagaaaaagg gtttacagaa tagattttaa
    38881 taaa
//
LOCUS       DAOQOS010000045        38852 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_423_length_38852_cov_5.965848, whole genome shotgun sequence.
ACCESSION   DAOQOS010000045 DAOQOS010000000
VERSION     DAOQOS010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 38852)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 38852)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38852
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_423_length_38852_cov_5.965848"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1006)
                     /locus_tag="PLF66_11610"
     CDS             complement(<1..1006)
                     /locus_tag="PLF66_11610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010569918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HMZ64735.1"
                     /translation="MDDKNKSTLIDFITAKEAILKELSSLESVSSGEISLPEISPEKN
                     EIKVEFQTSSIGPEQIVESIKTIKNHIENIRIHTFEPGFYVFQALNENVFDSKSILDN
                     IKFRFISSNNGNRVEVSKKGDFKREEIFAIIALFKYITGANSKKNANPKELLNRLGVN
                     VFDPAEEALSGNLMTFDHIAGYSGVKQEILESIVMPILSPYTFDQVSKLTRKFPTRNR
                     PRAVLFEGDPGVGKTTMAKVVSCLCKIPMVYVPIESILSKYYGESSQNLAYVFDAAAL
                     FPSSLIFLDEIDSLAGSREEGIVEATRKLLSVLLRKLDGFEGKPRTITIGATNRKQDL
                     D"
     gene            1113..1613
                     /gene="fliN"
                     /locus_tag="PLF66_11615"
     CDS             1113..1613
                     /gene="fliN"
                     /locus_tag="PLF66_11615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020765973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HMZ64736.1"
                     /translation="MADGSLSQEDIDALLGGGSFGGESSGGDSGASDDPFAGMNLGGG
                     GDGPSSDAIAAALGPGFPPPQAPKPSVGKIGGSMASSTSNLNLLMDVTMSLTVELGRT
                     NMYIKDVLQLSEGAVVELDKNIGDELDLLANGKIIGRGKIIIIDDYYGVQVTHIIDPM
                     ERLALG"
     gene            1620..2315
                     /locus_tag="PLF66_11620"
     CDS             1620..2315
                     /locus_tag="PLF66_11620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64737.1"
                     /translation="MTKQFTIILAVCLIAFTNCDKKQSKNFKFGTNISSKDVDTNNDG
                     KIDTKGYYMDDAGNYRLIYNELDRNVDGFSDLMIWVGSSAAPRKDAQVKDIVKVHEEE
                     DEDYDGKIDTLRWYLPNEFVALSQHDTDKDGYFESTKYYNFNKVEVRTEKDTNFDGRA
                     DIVSWLSRAELDTNFDGIPDTFTEAPTKLILNDKIERKVDLRSLDKSKSWFLNPTLIP
                     LEERSIIGSGFFQ"
     gene            complement(2325..3524)
                     /locus_tag="PLF66_11625"
     CDS             complement(2325..3524)
                     /locus_tag="PLF66_11625"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HMZ64738.1"
                     /translation="MTQIKIKEWQLLSLLACIQFANILDFVIMMPLSPKLMEAFSISP
                     AEFGFIVSSYAFSAGFAGILGAFFIDRFDRRFALNSCYAGLTIATLLCGLVHSYSLLL
                     MARILAGAFGGLTSAVTFAIIADLIPYERRGKATGIVMSAFSLASVIGVPIGLYLADL
                     YNWNAPFIGLTVFSCVVLVFAHFYTPSVKGHLSSMAKHPLKTLEVLFLNKNHLIAFSL
                     ITMMMFGGFSVIPFIAPYLVKNIGISNLELSYVYILGGAFTVFTSRLIGSLADKFGKP
                     RTFAIVAFISMIPILGITNLPKVSIYLTLAVTTCFFVFVSGRFTPAMAMISASVHPLN
                     RGSFMSMFSSVQQIASGLAASLSGFIIQKTPDGQLTEYPTVGLVAVIATIIAIWISTK
                     LKTVTAE"
     gene            complement(3524..3805)
                     /locus_tag="PLF66_11630"
     CDS             complement(3524..3805)
                     /locus_tag="PLF66_11630"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HMZ64739.1"
                     /translation="MPAKEKEISFEEALNELEQIAEKLERGQLTLEDSIKAYERGMEL
                     KKICTERLKEAEGKIEYLAKAPDGKIVKESAIKEAKEPKEKKNKEEILF"
     gene            complement(3806..5077)
                     /gene="xseA"
                     /locus_tag="PLF66_11635"
     CDS             complement(3806..5077)
                     /gene="xseA"
                     /locus_tag="PLF66_11635"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015679821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HMZ64740.1"
                     /translation="MSEVRPLSVSEVNRSIKSILLERRELKDIWVKGEISNYSPSGAG
                     HIYFSLKDQTSVIRCTFFSFQNKNYKGKKLQDGMEVQVFGSINLYEPGGYYSINVARV
                     EELGKGDILYRIEKLKAELNARGIFNPDRKRPIPRFPKTLGIATSPNGAAVEDIIRIA
                     KERYPNINILIAPCQVQGDTAPSSIVSAIEELNNPKWEVDVIIAGRGGGSFEDLMPFN
                     DESVVMAYYNSRVPIISAVGHQIDSLLCDFAADAFVPTPTAGAELAVPEIEELENFLE
                     DIENRFNQSLKYKLDIARDRLHLISNKHVFVEPRMLLTDRIQRVDEILNRIFLLGKNN
                     IAAKNNLLQKFDNIKIYAKSVLNAKDNQFQLTLGRVENFSPLETLKRGYSVVRNKKKE
                     VISSKSQVKVGEELELIMKDKETIKIEVKEK"
     gene            complement(5082..6248)
                     /locus_tag="PLF66_11640"
     CDS             complement(5082..6248)
                     /locus_tag="PLF66_11640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010419329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HMZ64741.1"
                     /translation="MRKIIALLKFFIADLVSSMGHGELKNSKYVLPVVLIVLFSIAST
                     FAFLVYQSYFWAAFISLILYVGSRDYYLKLKSRIPNRFKGAAPSIMILIVLITIVLPC
                     FFIIRTLLDELISLLFVLKVNLSEDRIVPTLMSINLITDYFTDTEFFWVQLPNMYREI
                     VSSYGDILNVDSLYGILSNTTSFILGGIRIPLAILVNICFSFMLLFFFYKDGHKLEVF
                     LMNNLPFSEEVEKQIGQRISEAVKAVLKGNILISLLQGFVAGVLLFFAGIPNPILYGS
                     IASFFSLIPVIGTAVVWLPAGLYIGFFEESWIVAVIYMSLSFTSYMVLENLVKPNILD
                     KKLNLHPFLLFLALLGGIQQFGIVGLVIGPIAVTILVILWDFWMAYRRKEFGGN"
     gene            complement(6196..6627)
                     /locus_tag="PLF66_11645"
     CDS             complement(6196..6627)
                     /locus_tag="PLF66_11645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64742.1"
                     /translation="MNKFISLLILSCLSIFAEELKEETFQFENLRVIKRPASLPNNVI
                     ASDFIPSGATILQAVELTNVTGMIPEAGLFLESKNTTEELIKFYENALASRDWRIIQK
                     DNKGNKSVLLGENPSKKVMTILIRDEKDYRVVKVFYRRSGF"
     gene            complement(6684..7250)
                     /locus_tag="PLF66_11650"
     CDS             complement(6684..7250)
                     /locus_tag="PLF66_11650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64743.1"
                     /translation="MEYLNRFLLFLFICLSFPLLFSQTQEEILEDLREDNSYKTETSQ
                     YSFLEKLRSIYKNVKSDLENKEDKNAIQHANELEKLGITSDKFSFRGKSELGEMETLY
                     LKIRKLRGEAFYNLRDFKNALSDSKFIVENHPKSVVFDYTRYAVSLYYSGEERQARKI
                     LNQAKAKFKNSNDQEILNKTLQLLFPNG"
     gene            complement(7234..7818)
                     /locus_tag="PLF66_11655"
     CDS             complement(7234..7818)
                     /locus_tag="PLF66_11655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64744.1"
                     /translation="MNFRFILLSLWGLIAVNCATFTEFNPEVKDVVFKNQNPAKSLLV
                     NVRQRVTINGTLSDVSAKGEENARNRYAYFCRESGLFKEVKTGLDIADMKLLIESDNN
                     GDANRLLAFLSGLTLFLIPAYAQDDLNLNFSFRDKNDTIIKEYKRRVTFNTWIHLILI
                     PLMPFKFPIVEVNDGVESITRSILDEANRDGIFK"
     gene            complement(7820..8413)
                     /locus_tag="PLF66_11660"
     CDS             complement(7820..8413)
                     /locus_tag="PLF66_11660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64745.1"
                     /translation="MNLLKEILLIHFLLIGCISLPPSATLLDPVKQQNTIPEKVVIEK
                     TNIPELSDRYRKATEDNLTMNWKDFLVKGNYFEKSILFTDKPEDWTNYKNIQLHFSKF
                     SQERTIDPWYFPLALVTLTIYIWIGGGVSIDTGIYNASLLVKDEKGKVIYQVSKEKME
                     ERSINIYNRYSMTSFAEVKTKLITELLEDYQLQKKGK"
     gene            8744..10093
                     /locus_tag="PLF66_11665"
     CDS             8744..10093
                     /locus_tag="PLF66_11665"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HMZ64746.1"
                     /translation="MIVDSEIIPILKNLELFSETPESSLKEIASVLQIESFSKNEKII
                     EQGDPGTCLYIVYSGRVFVHFGEHKVAEIGEKQTFGEFSLLNSEPRNASISALVDCKL
                     LRLEQVDFYRIMGHNNQFMRGIIRILIRRLGEQNNELIQTLKKREAELTRLVAERTKE
                     LELALKEIESQKVDLEKSYEEILKQKSEIEEKNLNITESIRYAHNIQGSILPPDALIK
                     KALPDSFIFFKPRDVISGDFYWFHESNAENDSKIVLAAIDCTGHGVPGALMSMIGNGL
                     LNDIVKVRHIIDPGSILNELHKGVRIALNQEQNGIRDGMDMALCVIDRKSGILEFAGA
                     MNSIFYIQNNTLNEIKGNRKSIGGTQSEEARIFSTNIIELNTTTAFYICSDGYYHQFG
                     GEFHKKFSSKRFKDLLLEIHLLPMDKQYTILQDTMTEWIGEGSQMDDMLVIGVKLSI"
     gene            complement(10388..13069)
                     /locus_tag="PLF66_11670"
     CDS             complement(10388..13069)
                     /locus_tag="PLF66_11670"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HMZ64747.1"
                     /translation="MNEENILPSDNPINFSKHDLHFQEIYDLSPFPLLILDREGHITF
                     INKNFSNLISMDSNEMTGRNIRSLITSDSLFSFDEFLESIHNTNSLKNCEVRIMVSNY
                     KIKFLKLEGVAENGKCIVTAIDITEMKMLELTSLSVQADLLSILEAIPELLFDVDLEG
                     KVFTFHSKRKDLLYFPADKIIGKQVNELLPPNVAGLCMNAIRHANEHGHTFGVQYDLE
                     IGQNLKSFEMSVSRKIQNYLDSPRFLVMCRDITSQKKIEIDLIRSEARYRGLFDNLNV
                     GTVVHSPDTSILMMNERASELLGEVKHEFLGKKSSDPEWHFILEDNTLCPQDLYPIHQ
                     ILKTKKPIQNKVFGIYRKRTRDIVWVNVNGFPILDKTNNILEIVISFIDITDRKKIED
                     TLRISEANMKAVLDNSTDAILFLNHKHKVQFFNLLAKLYIKNIFGIDLYHDLDMRNFL
                     ESGDLADFEENFGIALDGKIVTIERVYRYRRQKNWFFLQYAPVKNENNETVGVMIIAR
                     DIDPDKKIQLELKQSEIKFHTIFDKAPLGIILIESKSGKIIQSNQKFLDTIQYSTDEI
                     KNLTFQEIIYPDDLLQNLDKFRLFLDPSISLISMEIRCFRKDKSILWVNVNYVPFNIE
                     DIDTHYHIAIFSDITDFKITEEKNKAYLKTLEKLNATKDKFFNIIAHDLRNPFTGISG
                     LAEMLETKSSLESTQTAKENQRIAHLILTASKSAYALLENLMQWAKSQTGEIQFSPNI
                     ISLDSIIYSALQVVSVNAFKKNISIKTDIKDSPKVLADSSMTEAILRNLLTNAIKFSY
                     INGEIILRTKIVYNYLEISITDHGIGIEEENIEKLFRIDSKFTKLGTNSEKGTGLGLV
                     LCKEFVQKQGGDIWVTSKIGLGSTFVFTLPIGVITTS"
     gene            14051..14575
                     /locus_tag="PLF66_11675"
     CDS             14051..14575
                     /locus_tag="PLF66_11675"
                     /inference="COORDINATES: protein motif:HMM:NF019235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1564 family protein"
                     /protein_id="HMZ64748.1"
                     /translation="MVHSQNNSFSNRKIEFPRNDFQSSDFSSTLLIPSQHFLIFQEKI
                     AKHKGIRSYIAYLLVKYQLAISNGMIPGYSKVTTKYQEKGQNLHKVAFRPRPQDWAEL
                     KLYRISFGMSISAFIIYLLIADSVDFAETVSKYLESVGISSTPNLDLCSKVYLYNSKS
                     IYTIIFQYRESRDH"
     gene            complement(14572..15732)
                     /gene="hemW"
                     /locus_tag="PLF66_11680"
     CDS             complement(14572..15732)
                     /gene="hemW"
                     /locus_tag="PLF66_11680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HMZ64749.1"
                     /translation="MEIATYRKGFAGLYLHFPYCIHKCSYCDFYSVGIGKTDPPNQDI
                     LFNNYKKEFLLRIQIDPTILDYTFDTIFIGGGTPSLANLDLLKDFIEWVRSYLKFSPN
                     TEFTMEMNPEDISEEKLVQLFNLGVNRVNAGIQSFDKNLLATLDRYNDEDKYSSVLRI
                     LSSSPIKRFGIDLIYGIPGQTKDMFLSDLQNAISKKVTHISLYSLTMEKGTEYYRKVK
                     RKESLPPNEELQIEILNSLPTLLKEKEFRQYEVSNYALLGEESRHNLKYWTMEKYLSL
                     GPGAHGFTDNGRYFNHRSIEKYLSGEFGRNYEKSESLDELALCLFRLFLPIDLRSFFI
                     LIPDHVDDLEKLISIWQTQDLCTYKQGIFQWRQNAIIRLDDLILQVSELVNS"
     gene            complement(15735..17426)
                     /locus_tag="PLF66_11685"
     CDS             complement(15735..17426)
                     /locus_tag="PLF66_11685"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 protein"
                     /protein_id="HMZ64750.1"
                     /translation="MKIKKIIPFLIISFSISATDLTEIRKRALEHASEIVSKMSDEEK
                     AGQVIHIAIPKNYLDEVAIAELQKVRPGGVILFGVNLGKKKEIRALTKGLQNEMKNLN
                     SPPLFISIDQEGGRVVRVEKGVTGFPGALAIGQTKNPAYAFNVGLITSYELNNLGINL
                     LLAPVMDINNNPENPVINTRSFGMTLDAGDLAISYEKGARIGGAVPVIKHFPGHGDTN
                     VDSHLGLPVIEKTESQLLEFELIPFQKSIQEGARAVMSAHIVYPNLDPKYPSTLSKKI
                     LTDILRKKLGFEGIILTDAMEMHAISKNYQNEKTGTLAILAGADIILLTSWGNTTTHY
                     YNMILESLKNKEFDVDGKNMLDESLKRQIALKIENGLFHTSYSYKKIEDEELEKFITE
                     KRKISTARYNDLKIANIPELNARISTEAIRSYKTPFTSFTISESRKFAYVCKNENVKN
                     ELIDLNIPYYTEKKVQKLLKKKLVKTLVFDTKDQKELDAVSNLAEKNPSIQFILLHYG
                     SPFLKLPNQGNVKILFSFSPTKDSIHALVHTALNADKPRDIPVTELIFSRIIQRN"
     gene            complement(17595..18236)
                     /locus_tag="PLF66_11690"
     CDS             complement(17595..18236)
                     /locus_tag="PLF66_11690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64751.1"
                     /translation="MAAKFLITLCIFFTYGILAQAKSVASNASIMTADPGDALREKAL
                     QDSIKEFEAFINAKVSEIERLQTKIVTYDQRLRKVITIVNENTLSTFEEHQNYSVVRY
                     IEYEFESSKLKEVRFIYKKKSLRDNVIYVYRTMYMTPGNFDGIKIITEKVEQKAKGVT
                     TAENYKDFSADVKLRTLKTIDTNLLGSIYKMDGYLQNAAHEKDKKSHRELEGF"
     gene            complement(18254..18970)
                     /locus_tag="PLF66_11695"
     CDS             complement(18254..18970)
                     /locus_tag="PLF66_11695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64752.1"
                     /translation="MSVADKFIDEDNFMLFKTYIVISIFTTGLLFAQNQIKNSDKGEG
                     IGAVQQKSDKPKPVANSASAKAMQDKIASLEKEIIVKISDIEKLQAEAVEFNPEMKKE
                     IRIVYEQPKDHPIPGPQKYLVYRYVEYEMDGGSKIKEIRVISKHRSLMEDMHSIVKVV
                     SFKPNDMSSIKVTVDKLETKAKGIQEVVNYNDFAPEVKLQALKTIDTTLFNSIFRLDT
                     FIQRNQLSKNDKNRNQLEGL"
     gene            19097..19969
                     /locus_tag="PLF66_11700"
     CDS             19097..19969
                     /locus_tag="PLF66_11700"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HMZ64753.1"
                     /translation="MKSPKNYFIFLILTFLCSIFLTSCEKLADATFSDKDDIEMGASL
                     DKEIRSKPSEFRILENPELENYVQTIVKQLIRSPLVKRKKVYKYKVTLLNDDKAINAF
                     CTPGGYIYVYTGLLKFLENEASLAAVLSHEIAHAEKRHVRQRMLSALGIELIVSAILG
                     NGSSIFQEMGVKLAGTLALLSNSRADELEADEWGFLYLGNSPYYQGAMSYFFEKISKE
                     EKRSSASKALEKLLSTHPIPEERLKVNSERIRKAKIQPPIPSNLLESRYKKMIRKHLG
                     AGETHDGESIDDKG"
     gene            19972..20589
                     /locus_tag="PLF66_11705"
     CDS             19972..20589
                     /locus_tag="PLF66_11705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64754.1"
                     /translation="MIFETVNEINEAIKAINYASYLIRLCALNSQIAISSISFHSQKS
                     AGFTPISKELIHISSLIETKANDLFQIMFKSLRTITESQKIARNQSLQKKFFQDYNKR
                     SDRNYDLTHLENKILSDYNNKRDKLKEDFQIQFQSFQKIIRETIKLCKNTRPIVFSVK
                     IESVYIEGNEGAFDELSKELSDFISKIEDLLKRIENLNQRLLEGN"
     gene            20591..21340
                     /locus_tag="PLF66_11710"
     CDS             20591..21340
                     /locus_tag="PLF66_11710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020776265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HMZ64755.1"
                     /translation="MKKFQYIYSEGDHKWGIISRDPEKPSYLIDTNEYVICKGNKLII
                     TDPGGIEIFPSVITSISTEQNPIDIEYIFASHQDPDIISSLTLWLDINPSIKCFTSFL
                     WSTFLPHFGGNDSTFSIIPDIGSSFNFHGLELEFVPAHYLHSSGNFHLYDPKSKIYFS
                     GDIGAALLPEAVAKEGKIYVDDFDNHIAYCKGFHERWMGSNEAKLDWCNRVSKMDIEQ
                     LCPQHGMIFKEKDVMRFINWFSELKIGVLRS"
     gene            complement(21337..22668)
                     /locus_tag="PLF66_11715"
     CDS             complement(21337..22668)
                     /locus_tag="PLF66_11715"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HMZ64756.1"
                     /translation="MKQEDLLDAIPHYLLHSKSQIAFILDEQGKFLISNYLFKDTFGE
                     PKHQREFESIFEQNAKLENLIQQLKNDPQEIQSIDLRVKLNKLYKSISFEVSYLKNKD
                     KNIGYIFIGYLNLNESSSFLSLELKCKAILECTQEVFILIDRNGIILAFNKTAFDMIF
                     RTQGKELKESRPMIEFIMDTDLESFSKNFKTALLGNSVHFEKQFQFYGYSLWADISYL
                     PIRENENVIAVAFVAYSIDDKKMSELKMQQNENILTAIYNSSSEGTLFLDLDLRILFF
                     NKVAAATAQAIFQKELEIGTSALDYLSEYLKDDFLGYFHRAKKGESIVIEETDGIKWW
                     LFSMFPVYDENKNMIGIANNVQEITSEKENEFKIIHQRARLKEIAWQQSHRVRQHVAN
                     ILGLCNLFLNHKDRTEEEKDRSIEYIFKSARELDQVIHEIVRQTEEQSISI"
     gene            complement(22732..23214)
                     /locus_tag="PLF66_11720"
     CDS             complement(22732..23214)
                     /locus_tag="PLF66_11720"
                     /inference="COORDINATES: protein motif:HMM:NF013777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HMZ64757.1"
                     /translation="MKNILRFCKGKNKKNNIAWILDTPNSENGMTVYKLKDMVHYCPF
                     ELTLSILNGKWKPVILFKLMQNKTLRYGDLKKALPKTSDKMLSKELKDLEEFGIISRK
                     AYSETPPRVEYSLTKKGEKLKPIFGLMRKWGSSYKVVTEEDPSQTDKRIAVKILATEV
                     "
     gene            23272..24018
                     /locus_tag="PLF66_11725"
     CDS             23272..24018
                     /locus_tag="PLF66_11725"
                     /inference="COORDINATES: protein motif:HMM:NF014068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HMZ64758.1"
                     /translation="MSANLNVMNPDKKKKILIVAANASTSKTTGWPIGFWWAELTHPY
                     WEFVEKGFEVEIISPKGGDLLTDGYSDPEDASGYSAHDILSLGFKKSPKHAELIKNTK
                     SIKDVKISDYDALFIAGGQSPMYTMIDDTELHKFVADFYEAGKVVSIVCHGTCVLLKV
                     KLSNGKLLVDGKTWTGFANSEEQFADNFVGKRIQPFWIEEEAKKIPNTNFIVDGMFKP
                     FAVRDGRLITGQQQYSGAAAAKLVIEALGV"
     gene            24168..24785
                     /locus_tag="PLF66_11730"
     CDS             24168..24785
                     /locus_tag="PLF66_11730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HMZ64759.1"
                     /translation="MKIGIIGTGNVGGALATKWAQKGHEIFLGVKDTNSFKGKELLSN
                     PNTKVLPTQEAVKQAEVILIATPATAAIEVAKSLGDTTGKIIIDAMNVVMGKGPSGFS
                     NTADAILANTQTKDVVKCFNTTGFNNMENPIYGNVGIDMFMAGSSEKGKEITRKLALD
                     AGFGECYNVGGNDKFQLMEQFAFFWINLAMMQGLGRNIGFKLLKR"
     gene            24787..25725
                     /locus_tag="PLF66_11735"
     CDS             24787..25725
                     /locus_tag="PLF66_11735"
                     /inference="COORDINATES: protein motif:HMM:NF013246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family ion channel"
                     /protein_id="HMZ64760.1"
                     /translation="MIVKKNFNPIKVFSYIKYEMIYTSILAATVYYLHTNLLTEKISI
                     PFSIAGLLGGALAIFLGFRNNTSYARWWEARQLWSNIASSSRILARLILTFTDSHSHQ
                     QNYVKERSELFKKEMIYKQIAWVHSLRLNLRKENSWEEIKPLLPESEWTQLQTKTNKT
                     GFIQFLMGQRIYKAMADGTLGGFDSFQMEGQLLSLSNYQSGCERTKDTPLLKQYDFFT
                     RVFLVVFISVLPFTLLSELQRLNVIYLLIPITILISFVFTIVGKVGKVNEDPFENKIT
                     DVPLNSICNSIELELREMLEDKNLPKKIEPVDGYLN"
     gene            complement(25867..27027)
                     /locus_tag="PLF66_11740"
     CDS             complement(25867..27027)
                     /locus_tag="PLF66_11740"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HMZ64761.1"
                     /translation="MEKSPTHILIVDDVGENLQVLGNILSKEGFDTSFALNGKQAVSI
                     VEDTMPDLILLDIAMPVMDGFEVCKLLKSQENTKAIPIIFLTAKTEMDNMIHGFSIGA
                     ADYVTKPFNTLELLARVRAHVELKKSKDIILEQNKELSVLVAKLSEKNTELNSLIAAK
                     DKFFSIIAHDLKGPVGNLNSFLSFMAAQYENISKEEFHEDLILLESASNKIKGLLENL
                     LTWARSQRGDMQYKPESISIYDVVKSSIQLFEPSAANKKILLNNKVSTDIKAFADSQM
                     ITTVIRNLIGNAIKYTDENGSVTISANESGGKVELTVEDSGIGMSSNIAENLFRIDVK
                     HFSTEGTNGEKGTGLGLILCKEFIDRHQGRIWAESELGKGSKFRFTIPITAS"
     gene            complement(27028..29367)
                     /locus_tag="PLF66_11745"
     CDS             complement(27028..29367)
                     /locus_tag="PLF66_11745"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HMZ64762.1"
                     /translation="MLKREKRILIVIDFNEDKILLQNVLQKEGYILDFISSDELWIHQ
                     NESNPDLIILDIEVNQERNTLDRLKAAHTTKDTPVIYINHLRDSLHPLSILEIGCTDF
                     ISKPLNAAELVVRVHNQINFNSLQKRLEFTNTALEESVLRRTAELEEANRRLLVSEER
                     WQFALEGSGDGVWDWNLETNEVFFSKRWKEMLGYNENDRWDTYEEWTRHIHPDDVGWV
                     TDNIQLHLSGATLSYESEHRVVKKDGNYIWVLARGKVIQSIKGVPSRMVGTHTDITDR
                     KLIEENLQKAKAEAEVANRLKSQFLANMSHEIRTPMNAILGFAEILKDKIGANPDLLE
                     YLVGIQKSGRNLMNLINDILDIAKIEAGRLEIVNLPVNLNSIISDIRQMFSIQVSQKK
                     LGFEIAIDDHLPESILLDELRLRQVLFNLIGNAIKFTEKGGIRINVRCIKNNSDDNMV
                     DLIFEVIDSGIGISSNEIATIFDPFKQQQGQSNLKYGGSGLGLSIAKRLVEMMKGRIT
                     LESKIGKGTKFTIFLPKIEISDQPAITNTEVSRDTASVKFVQARVLLVEDNEPNRQVV
                     VGFLENSNLKIKEAFNGRMALDMIYQEPFDLILMDIHMPEMGGKETTQIIKQNEKYKK
                     IPVIILTAFARNEDVLEMKGFADAVLTKPITKAKLISEVARFIPTLESETIVTQQGLT
                     EDNFIEELEKLILKQKISTEFKSQYFVWYRESEIARKSLNTNRLRTFIIDLVHISDTF
                     NMELFKQYANYLNDLIHSFAIGKLSNALNQLEIAFERLR"
     gene            complement(29369..30364)
                     /locus_tag="PLF66_11750"
     CDS             complement(29369..30364)
                     /locus_tag="PLF66_11750"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HMZ64763.1"
                     /translation="MEIRDTGIGIAHYELDSIFSPFIQQSGQDAMRFGGSGLGLSITR
                     KLVEMMGGSITVDSKLGAGSSFRVFIRELEISSLAHNSKEKDFEISNIAFEKAKILLV
                     EDIESNRRVVTGFLAQWNFQILEAANGRLALDILEREMVNIILMDMQMPEMDGKTASL
                     LIRSNERLKKIPILVLTATAMKEMVDDILTFADGYLCKPISRVELIRELARFLPNTKK
                     EETEIHIKVNSDTFLEDIKKILLEKNYNPDFISKLKNWFLETETARKSIQTNKLTIYI
                     QELKEIGEANHIIPLVGFSLELLQLIKNFSIPQVAIKLQILEQLHFIILSLSEDH"
     gene            complement(30373..31965)
                     /locus_tag="PLF66_11755"
     CDS             complement(30373..31965)
                     /locus_tag="PLF66_11755"
                     /inference="COORDINATES: protein motif:HMM:NF024140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HMZ64764.1"
                     /translation="MLDFLKGRNQSKIVKTISFWYIVIFFCLFVIALIAVPAILRLDS
                     IAKDIYIHPFTVSNAAIELRYNVARIRNIMLKSFFSSNKNEIENLSREVNEIENTVQK
                     NLESIRENFLGDQSKVDEIDRLLSNWKQMREEEFEYLISGRVGKAAELRNRVEEEIYL
                     PINELTNYIIGFARNKVVIFVTESEMTMNRVIISLFCLILLILLSGFFILIRVIKLQR
                     ESENAINIEREQYLIEARIFAEEELIRNKYKLKILADNVPAIVSMVDKNLKFQFVNKR
                     FAEYFKTSSEECVGKNVREIVGDRIFKQVEENYYKALAGESITFESVFDFKDGEERYF
                     EIKYTPFIEDGKQTGIIVLSHDITDRKLSEINLLNAKEGAESANRLKSEFLANMSHEI
                     RTPMNAILGFSEILKDKIGNNPVVLDYLSGIQRSGKNLISLINDILDLAKIEAGKLEI
                     TYSPVNLFNVIYDIQQIFSLQTAQKNLKFEISIDETLPKSLLIDELRLRQVLFNLIGN
                     AVKFTEKGGIYNPFQEEARVVV"
     gene            32095..32967
                     /gene="ppk2"
                     /locus_tag="PLF66_11760"
     CDS             32095..32967
                     /gene="ppk2"
                     /locus_tag="PLF66_11760"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002774720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HMZ64765.1"
                     /translation="MGSENEEKIDDEKKEKKDKKKPKRFTSEGEGSSKLSSKKYEKHL
                     AKLQLELVKMQYWVKYAGAKIVIIFEGRDAAGKGGTIKRIVEPLNPRGCRVVALGTPS
                     DREKTQWYFQRYIPHLPAAGEIVIFDRSWYNRAGVEHVMGFCTEEQYRDFMQDCPEFE
                     RMLVRSGIILLKYWFSVSDDEQERRFQSRTTDPARRWKLSPMDLESRDKWVEYSKAKD
                     AMFSHTNIPEAPWFTVEADDKKRARLNCLHHLLSKIPYLDMTPDPLILPPRKSADDYV
                     RPPRNEQFFVPEVY"
     gene            complement(33145..33963)
                     /gene="lpxA"
                     /locus_tag="PLF66_11765"
     CDS             complement(33145..33963)
                     /gene="lpxA"
                     /locus_tag="PLF66_11765"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: protein motif:HMM:NF003657.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HMZ64766.1"
                     /translation="MSSILFKPGIHPTAVIESGATIGNNVSIGAYSFIGPKVTIKDNV
                     RIGQNVIIAGKTTIAEGNTIYPINVLGTPGQDLKYTDYEDGILNIGQNNIIREKCSIH
                     ASTPGHETVIGNNNFIMAETHIGHDCHVGNFVVLASVGLAGHTEIQDYAVLGAKSGFH
                     QFCRIGRYAMIAGVAAITKDVPPFALSEHERPNEFSGLNIIGLKRKGFTKDDIHRIRE
                     IYEFLYDMTGAMENRIQPLREKFPNDKFATEIADFIEKSKRNLCVPGSKNPRRK"
     gene            complement(34484..35416)
                     /locus_tag="PLF66_11770"
     CDS             complement(34484..35416)
                     /locus_tag="PLF66_11770"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HMZ64767.1"
                     /translation="MLRIILIIIFIILGNCSGKEKEYHPRAVVIFRTGDVRTTAKSLA
                     LGDEITDTDLVMVGGKSVCDLQMIGTETLAVIRIKEYSKFKLKGVQVGFTKTNNFIVD
                     SGSAMLNVSKLGRNEEVNASTPTLTAGVRGTKYDLNVAPNGNSKVNVVEGSVSMKVRV
                     PELEKYSRTEIKQSKTLSAVDDALESKEIVIESGKSSQVPKSVSTKILKDTGLETVIK
                     KDSASEVDKDVDPKAVEEKLEKLEKEDLNNTTSDIDQASMTQKLKEYEELTPIEKEII
                     NDKEKRKSVIKARSEKLEKSWLQRLLDWSKNIKF"
     gene            35848..36480
                     /locus_tag="PLF66_11775"
     CDS             35848..36480
                     /locus_tag="PLF66_11775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HMZ64768.1"
                     /translation="MKYNLFFLCILTLFYCNTPPKREATKNPKEEYDHCYIKSFYPAE
                     YKTIEDRILIAPASIKEETIPALIKTVEETVIDKPGKSVWVAKENDRYCFEEIPPTYK
                     TIKRDVLISPETTKMVEHPDEYKVVTRKELLQEARVEWTEILCENKYTPELIKKIQRS
                     LQAKGYPTGREAELDNETFTAINKFQKANQLKVNKTKSIYLETIQALGVK"
     gene            complement(37101..>38852)
                     /locus_tag="PLF66_11780"
     CDS             complement(37101..>38852)
                     /locus_tag="PLF66_11780"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HMZ64769.1"
                     /translation="EKLSNSKENDLAIAEYSSSFFKEKSIEKLNKIINLYLLQNQKEA
                     CLSFLKKAERESEDNTDLLEQIAFYYLKLNQITTGIGLYKKILEKNPESYRSYYQLGL
                     NSLEKDRRLSLSYFEKAIAIERHDSNLYIARGINYYKLGNRERAGEDFRYALSLESDL
                     EIASYNLGILLYNDNLIQDAELIFLDLTKKYPNFQEPFYHLSYIYFERKDFSKAEKYI
                     LKSLSIDRNAASLYAYIQILKELQNEKDKTKITKSIQSLKKELIDNFPQSSYAKNLST
                     ETIGDRSEKVSIQSYPLEDMIQSQPIYINQSLIVNYGTYITRIHSEKKSILWRIETPS
                     SYRILKANTRLYGLSKSSLDQFDLETGKLLWSIPMRKNAIIRFQISDTILYSEKIAGV
                     ETLYSYSMDGEFLSSISIPPKSKWELTITGKLYIFHNNSDGMSWEIYDSKLQPLTESF
                     TLLSAEAEIRIIGTIDDSCYLLRENTIYRFNAKGEFLNSGKLDEKATLYIYKKYIYMK
                     TQKNLYIVSNNLDQFKKMEGVEVNQSEFPIDNNTYLSSDGIIKVRDQSGKILWSENLS
                     KRADKTKSSAYSVYIAE"
BASE COUNT        12840 a         6833 c         6008 g        13171 t
ORIGIN      
        1 tatctaaatc ttgctttcta tttgttgcgc ctatcgttat cgttctaggc tttccttcaa
       61 atccatctaa cttgcgaagt aaaacagaaa gtaatttccg agtagcttct actatacctt
      121 cttccctact tcctgcgaga gaatcaattt catctagaaa aattagagag gagggaaata
      181 gagcagcagc atcaaataca tacgctaaat tttgagaact ttctccgtag tatttactta
      241 aaatagattc aattggaaca tagaccatag gaattttgca aagacaagaa actacttttg
      301 ccattgtagt ctttccgacc ccaggatctc cttcgaacaa aacagctctt ggtctatttc
      361 gtgtcggaaa ctttcttgta agtttggaaa cttggtcaaa tgtatatgga cttaagattg
      421 gcattacaat cgattctaaa atttcctgtt tcacaccaga ataaccggca atatgatcaa
      481 aggtcattaa gttaccagaa agtgcctctt cggctggatc aaatacattt acaccaagac
      541 gatttaaaag ctcttttggg ttcgcatttt ttttggaatt tgcaccggta atgtatttaa
      601 agagagctat gattgcaaaa atctcttctc ttttaaaatc ccctttttta gagacttcta
      661 ctctgttccc attatttgag gatataaagc gaaacttaat gttatctaaa atggacttac
      721 tatcaaagac attttcattc aaggcttgaa atacataaaa tcctggctca aatgtatgaa
      781 ttcgaatatt ctctatgtga tttttaatcg tcttgataga ctctacaatt tgttctggtc
      841 ctatagaact ggtctgaaat tcaactttga tttcattttt ttcaggggaa atctcaggaa
      901 ggctaatttc accagaggaa acagattcca aagaagagag ttcctttaaa atagcctctt
      961 ttgcggtaat aaaatcaata agggtagatt tatttttatc gtccatggta ctaattttac
     1021 cgttattaaa aatgtctttg tatcgtcttt tattctttac ttgacaaggt atttttttta
     1081 taaaaagttc aaaacacaga ggtatttgat ttatggcaga tggttccctt tcacaagaag
     1141 atatagatgc actcctaggc ggaggaagtt ttggtggcga gagtagtggt ggggactcag
     1201 gtgcgagtga tgatccattt gcaggaatga atcttggcgg tggaggcgat ggtccgagtt
     1261 ccgacgcaat tgccgctgca ttaggtccag gttttccacc tccgcaggcg cctaaaccaa
     1321 gtgttggaaa gatcggtggt tcaatggctt cctccacatc gaacttaaat ctacttatgg
     1381 atgttaccat gtctctcaca gtagaacttg gtagaacaaa tatgtatata aaggacgttt
     1441 tacaactttc tgaaggcgct gtagttgaat tagataaaaa tattggggat gaactcgatc
     1501 tactcgcgaa tggtaaaatc attggacgag gtaaaataat aatcattgat gactactatg
     1561 gagtacaagt aacgcacata attgatccaa tggaaagatt agctctgggc taaaaatata
     1621 tgacaaaaca attcacaatt atactcgcag tttgccttat tgcatttaca aactgtgata
     1681 aaaaacaatc caaaaacttc aaatttggaa ccaatatcag ttcaaaagac gttgatacaa
     1741 acaatgatgg taaaattgat acaaaaggct attatatgga tgacgctggc aactatcgtc
     1801 ttatctacaa tgagctagat agaaatgtag atggattcag tgatttgatg atctgggtag
     1861 gatcttccgc tgcgccaaga aaagatgcac aagtaaaaga tattgttaaa gtccatgaag
     1921 aagaagatga ggattatgat gggaaaatag atactttgcg ttggtatctg ccaaacgaat
     1981 ttgttgcact atcccaacat gatacagata aagatggata ctttgaatcc actaaatatt
     2041 ataattttaa caaagtggaa gttaggaccg agaaggatac aaacttcgat ggaagagctg
     2101 atattgtttc ttggttaagt cgagctgaac tagatacaaa ctttgatgga attcctgata
     2161 catttacgga ggctccaaca aaattaatct taaatgacaa aatcgaaaga aaagtagatt
     2221 tgagatcact cgataaatct aaatcttggt ttttaaatcc aacactcatt ccattagaag
     2281 aaagatctat tattggaagt ggcttttttc aataagccac tactctactc tgctgtaaca
     2341 gttttcaatt tagtagaaat ccagattgca attattgtag cgataaccgc aaccagaccc
     2401 accgtaggat actcagtcaa ttgaccgtcc ggagtttttt gaataataaa acctgataag
     2461 cttgcagcaa gacctgaagc tatctgttga acagaggaaa acatactcat aaaactccct
     2521 cgattgagtg gatgaacact tgcagaaatc attgccattg caggagtaaa tcttcccgag
     2581 acaaaaacaa aaaagcaagt tgtaacagca agtgttagat aaatagatac ctttggtaaa
     2641 tttgtaatac cgaggattgg aatcattgag ataaaggcaa ctatggcaaa agttcgcggc
     2701 ttaccaaact tatccgcaag cgatccaatg agtctagaag taaataccgt aaaggctccc
     2761 cctaggatat aaacataact caattctaag tttgaaattc caatgttttt taccagataa
     2821 ggagcgataa acggaattac tgagaatcct ccaaacatca tcattgtaat aaggctaaat
     2881 gctattaaat gatttttatt taagaagaga acctctagtg tcttgagcgg atgctttgcc
     2941 atactagata aatgtccttt tacggatgga gtataaaaat gagcaaaaac caaaacgaca
     3001 caactaaaaa ccgttagtcc aatgaaagga gcattccaat tatataaatc agctaaataa
     3061 agtccaatag gaactccaat aacagatgca agagaaaagg cagacataac aatacctgtc
     3121 gctttgcctc ttctctcata tggaatcaaa tctgcaatga tggcaaatgt aacagcgctt
     3181 gttagcccac caaatgcacc tgctaggatt ctagccatca gaagaagtga ataggaatga
     3241 actaatccac aaagaagagt cgcaatggtg agtcccgcat aacaactatt taacgcaaat
     3301 ctacgatcaa agcgatcaat gaaaaatgct cctagaatac ctgcaaatcc agcactaaat
     3361 gcataagaag aaacgatgaa tccaaattca gccggactaa tactgaatgc ttccataagc
     3421 tttggactca aaggcatcat gattacgaaa tccagtatat ttgcgaactg aatgcaagcg
     3481 agcaaggaaa gcaattgcca ttctttaatt ttaatttgtg tcattagaat aatatctctt
     3541 ctttattttt tttctcttta ggttcttttg cttccttgat tgcactttct tttacaattt
     3601 taccatctgg ggctttagca aggtattcaa tctttccttc tgcttctttg agtctttctg
     3661 tgcagatttt ttttaattcc attcctctct catatgcttt gattgaatcc tctagtgtga
     3721 gttgacctct ctctaatttt tcagcaattt gttctagttc gtttaacgct tcttcaaatg
     3781 agatttcttt ttcttttgca ggcatttatt tctccttcac ttcaatttta attgtttctt
     3841 tgtctttcat gataagttct aattcttctc ccactttcac ttgcgattta gatgagatga
     3901 cttctttctt tttatttcga acaacagaat aaccccgctt caaagtctcg agaggagaaa
     3961 aattttctac tcttccgaga gtcaattgga actgattgtc tttggcattt aaaacagatt
     4021 tggcataaat tttaatatta tcaaattttt ggagtaaatt attcttagcc gcaatattat
     4081 tttttccaag cagaaaaatt cggtttagaa tttcatcgac tctttgaatc cgatctgtga
     4141 gaagcattct aggctccaca aaaacatgct tattagaaat taaatgcaat cgatcccgag
     4201 caatatccaa tttatattta agagactgat tgaatctgtt ttcaatatct tctagaaaat
     4261 tttctaactc ttctatttcg ggaactgcaa gttctgctcc agcagtcggt gtgggaacaa
     4321 acgcatccgc cgcaaaatcg cagagcagag aatcaatttg atgtccaaca gcagagataa
     4381 tcggaactct tgaattgtaa taagccatta caacagactc gtcgttaaaa ggcatcaaat
     4441 cctcaaaact tcccccgcct ctacctgcaa taattacatc tacttcccac tttggattgt
     4501 ttaattcttc gatagcactc acgatagaag aaggtgcagt atccccttgc acttggcaag
     4561 gagcaatcag aatattgata tttgggtatc tttcttttgc gattcgaatg atatcttcta
     4621 cggcagcacc gttaggcgat gtcgctatgc ccaatgtctt tgggaatcgc ggaattggtc
     4681 ttttacggtc tggattaaaa atacccctgg catttaattc ggctttgagt ttctcgattc
     4741 gatagagaat atctcccttt ccgagttctt ccacccgagc gacattaatg ctataatacc
     4801 cgcctggctc atagagattg atacttccaa agacttggac ttccatccca tcttgcaatt
     4861 ttttaccttt gtagttctta ttttgaaaag agaagaaagt gcaacggata acacttgttt
     4921 gatccttgag ggagaaataa atatgtccag caccagaggg actataatta gaaatttccc
     4981 cctttaccca gatgtctttt aattcacggc gttctagtaa aatggatttg atagaacgat
     5041 tgacttcact gactgagaga ggacgaactt cagacacaga actaatttcc accaaattct
     5101 tttcttctat atgccatcca gaaatcccag agaatcacta aaattgttac agcaattgga
     5161 ccaatgacga gtcctacaat tccaaattgt tgaattcctc caaggagtgc aaggaataat
     5221 aagaagggat gaaggtttaa ttttttatcg aggatatttg gttttacaag attttctaaa
     5281 accatgtaag atgtaaacga tagactcata tagatcactg ctacaatcca gctttcttca
     5341 aaaaaaccaa tgtagagtcc ggctggaagc cacacaacag cggttccaat cacaggaatt
     5401 aatgaaaaga aagaagcaat gcttccataa agaattggat ttgggattcc tgcaaaaaat
     5461 aaaagaactc ccgctacaaa tccctgtaaa agagagatga gtatatttcc cttaagaact
     5521 gcttttacag cttcagaaat tctttgtcct atttgttttt ccacttcttc cgaaaaaggt
     5581 agattattca taagaaaaac ttctaatttg tgtccatctt tataaaagaa aaatagaagc
     5641 ataaaagaaa agcaaatatt tacaagaatc gcaagaggaa ttcgaattcc tcctaaaata
     5701 aaggaagtag tattactcag aattccatag agactatcta catttagaat atctccatag
     5761 gaactaacga tctctcgata catgttagga agctgcaccc aaaaaaattc tgtatcggta
     5821 aaataatcag taattaaatt aatactcata agtgtaggaa caattctatc ctcacttaga
     5881 tttaccttta aaacaaataa gagagagatt aactcgtcga gcaaggttcg aatgataaag
     5941 aaacaaggga ggacaatcgt aatcaaaaca ataagaatca tgatacttgg tgcggctccc
     6001 ttgaaacgat ttgggattct actttttaat ttcaaatagt aatccctaga acctacataa
     6061 agtataagag aaatgaatgc tgcccaaaaa taggattgat aaactagaaa ggcaaatgtg
     6121 ctagcaatag aaaataaaac aattaatacg acaggcaata cgtatttact attttttaat
     6181 tctccgtgac ccatactaga aaccagatct gcgataaaaa actttaacaa cgcgataatc
     6241 tttctcatct cgaattaata tagtcataac ttttttggat gggttttcac caagtaaaac
     6301 agatttgttt cctttgttat ctttttggat gatgcgccaa tctctagatg ctaaagcatt
     6361 ttcgtaaaat tttataagtt cttctgttgt atttttagat tctagaaaca atccggcttc
     6421 cggaatcata ccagtaacat ttgtaagttc tacagcttgc agaatggtag ctccactagg
     6481 gatgaaatca cttgcgatta cattgttagg aagagaggct ggtcttttga ttactcttaa
     6541 gttttcaaat tgaaatgtct cttctttgag ttcttctgca aatatggaaa gacaagataa
     6601 aattaaaagg gatataaatt tattcatgga taccttagag tttttagtat ttttgaatga
     6661 aggtaagcaa gcaacaaaaa tttttatcca ttcggaaaga gaagctgtaa tgttttattt
     6721 agaatctctt ggtcattcga atttttaaat ttcgctttgg cttggttgag tattttacgg
     6781 gcttgccttt cctctcctga ataatagaga gagactgcat aacgggtata atcaaataca
     6841 acggatttag gatggttttc cacaataaac ttcgaatcgg agagagcatt tttaaagtct
     6901 cgtaaattat aaaaagcttc ccctcttaat tttcgaattt tcagatataa tgtttccatc
     6961 tcgcctaatt ctgatttgcc gcgaaaacta aatttgtcgg aagtaattcc taatttttct
     7021 aactcatttg catgttgaat tgcattcttg tcctctttat tctctagatc tgatttaaca
     7081 tttttgtata tagatcgtag tttttccaga aacgaatatt gggatgtttc cgttttataa
     7141 gaattgtcct cccttagatc ctctaggatt tcttcctgtg tttgggaaaa taaaagagga
     7201 aaagaaagac aaataaaaag aaacagtaaa aatctattta aatattccat ctcggtttgc
     7261 ttcatctaaa attgatcttg tgattgattc gaccccatca ttgacctcta caatgggaaa
     7321 tttaaaaggc atgagtggaa ttagaatcaa atgaatccat gtattgaatg taactcttcg
     7381 cttgtattct tttatgattg tatcattttt atctcggaaa gaaaagttga gatttaaatc
     7441 atcttgtgcg taagcgggaa ttagaaagag agttaagcct gaaagaaaag ccaaaagtcg
     7501 atttgcatct ccattattat cagattcaat cagtagcttc atatcggcaa tatctagacc
     7561 tgtttttact tccttaaaga gtccagactc tctacaaaaa taggcatatc ggtttctggc
     7621 attttcttcc ccttttgcag atacatctga taaggtccca ttgattgtaa ctcgctgtct
     7681 aacgtttact aaaagtgatt ttgcaggatt ttgattttta aaaactacat ccttaacttc
     7741 tggattaaat tcagtaaagg ttgcgcaatt aacagctatt agtccccaaa gacttagtag
     7801 aataaatcga aagttcattt tattttccct ttttttgtaa ttgataatct tctaacaatt
     7861 cggtaattaa tttagttttt acttcggcaa aactagtcat agaataacga ttgtatatat
     7921 tgatacttcg ctcttccatt ttctctttag atacttgata gatcacttta cctttttcat
     7981 cttttacaag taggcttgca ttgtatattc ctgtatcaat agagactccc ccaccaatcc
     8041 aaatatagat tgtaagtgtg accaaggcga gtgggaaata ccaaggatca attgttctct
     8101 cctgtgaaaa tttagagaaa tggagttgga tatttttata gtttgtccag tcctctggct
     8161 tatcagtaaa aagaattgat ttttcaaaat aattaccttt taccaaaaag tccttccaat
     8221 tcattgttaa attatcttct gtagcttttc tgtatctatc agacaactca ggaatattcg
     8281 ttttttcaat gacaaccttt tcaggaatgg tattttgctg tttcacggga tcgagtagcg
     8341 tagctgaggg aggcaatgag atacatccaa ttaagaggaa atggatgagt aagatttctt
     8401 ttaaaagatt catattcact taaaagtatc tatgtaattt ttatgtcaag cgactctatt
     8461 aaattcaaat tataaataaa gcttttatca aaaataataa accaatcaaa taaaaatgaa
     8521 aaaaagattt aaatgtgttt atacaattct tttcgttttt ttacaactac tatgaattgc
     8581 aactatcgca tatagcgagt tagaatataa ataatcaaat ttaaatccag attagaaaaa
     8641 cttcgataca aagaatagaa ggaaaaagag aagaattcaa ggatcagaaa aaaaatcgat
     8701 ttgactcatt gataagatag attatactat gtgaaaattt caaatgatag tagatagcga
     8761 aataattccg attctaaaaa acctagaact atttagtgaa acacctgaat cctctctgaa
     8821 agaaattgca tccgttttac aaatagaatc tttctctaaa aatgaaaaaa tcatcgagca
     8881 aggagatcct ggaacttgtc tttatatagt gtattcagga agggtctttg ttcactttgg
     8941 tgaacacaaa gttgcagaga ttggagaaaa gcagacattt ggagaatttt cacttctaaa
     9001 ctcagaacca agaaatgcat caatcagtgc cttagttgac tgtaaactgc ttcgattaga
     9061 gcaagttgat ttttatcgaa tcatgggtca caacaatcaa tttatgagag gaatcattcg
     9121 aatcctaatt cgaagacttg gcgaacaaaa caatgaattg attcaaactt taaaaaaacg
     9181 ggaagcagag ctcacaaggc ttgttgcaga aagaacaaaa gaattagagc ttgccctaaa
     9241 agaaattgaa tcccaaaaag tagacttaga aaaatcatat gaggaaattc taaaacagaa
     9301 gtcagaaatc gaagagaaaa atctaaacat aacagagagt atccgctacg cccataatat
     9361 tcaaggatct attctaccgc ctgacgcact aatcaaaaaa gcacttcctg attcatttat
     9421 tttctttaag ccaagagatg tgataagcgg ggatttctat tggtttcacg aaagcaatgc
     9481 agaaaatgac tctaagatag ttcttgcggc tattgattgc acaggtcatg gcgttcctgg
     9541 agctcttatg tcgatgatag gaaatggtct tttaaatgat attgtaaaag taaggcatat
     9601 aatagaccct ggatccattt taaatgaact tcataaagga gttcgaattg cactcaatca
     9661 ggaacaaaat gggattcgag atggaatgga tatggcactc tgtgtgattg acagaaagag
     9721 tggaatccta gaattcgcag gtgcgatgaa ttcgattttt tatatacaaa ataatacgtt
     9781 aaacgaaatt aagggaaata gaaagtcaat cggtggaaca cagagtgaag aagcaagaat
     9841 tttctctacc aatatcattg aactaaacac aacaactgca ttttacatct gttcagacgg
     9901 atactatcat caatttggtg gggagtttca taaaaagttc tcttctaaga gatttaaaga
     9961 tcttttatta gaaattcatt tattaccgat ggataaacaa tatactatac ttcaggacac
    10021 aatgacggaa tggattggag aaggaagtca gatggatgat atgttagtaa ttggagttaa
    10081 actttctata taattttgat ttttaattta taaaaatcag tttagaatcg attcttaaaa
    10141 actaattctt taatattcaa atcagtatta atctactcaa atcaaagaat ttaacgtgaa
    10201 tacgagataa gaagatgttt cattttgtca tccccgaaat tttatatcag gggtctctcg
    10261 aaattatcgt tatacatact cccgataagg ggatttcgga aatgacagct ttatagaatg
    10321 acggttcgaa taatttgtca gttgattatg ccgaactcac gttaatttag atagtttcta
    10381 aaagaaatta actagtagta ataactccaa ttggtagagt aaaaacaaac gttgagccta
    10441 aaccaatttt acttgtaacc caaatatctc ctccttgttt ttgaacaaat tctttgcaaa
    10501 gaactaaacc gagaccagtt cctttttcag aattagttcc taacttggta aattttgagt
    10561 caattcgaaa taacttttca atgttctcct cttcaattcc gatcccgtga tcagtaattg
    10621 aaatttctaa ataattataa actatttttg ttcgaagtat tatttctcca ttgatataac
    10681 tgaatttgat agcgttagtg agtaaattac gaagaattgc ttcagtcata gaagaatcag
    10741 ctaagacttt agggctgtct tttatatctg tttttataga tatatttttc ttaaatgcat
    10801 ttacactaac gacctgaaga gctgaataaa taatggaatc aagagaaata atatttgggg
    10861 aaaattgaat ttcaccggtt tgactctttg cccattgcat tagattttct agaagagcat
    10921 aggctgattt agaagcagtt agaattaaat gggcaatcct ttgattttct tttgctgtct
    10981 gagttgattc gagacttgat ttcgtttcaa gcatttcggc aagacctgag attcctgtaa
    11041 atgggttacg taagtcatgt gctataatat tgaaaaactt atcttttgta gcatttaact
    11101 tttctaatgt ttttagataa gctttgtttt tttcttctgt tattttaaaa tcagtaatat
    11161 cagaaaagat agcaatatga tagtgagtat cgatgtcctc aatattaaac ggaacatagt
    11221 ttacatttac ccaaagaata cttttatctt ttcgaaaaca tcttatttcc attgagataa
    11281 gagatatact cggatctaag aataaacgaa atttatctag gttttgtaat agatcatctg
    11341 gataaataat ttcttgaaac gttaggttct ttatttcatc agtagagtat tgaattgtat
    11401 ccagaaattt ttgatttgac tggataattt tacctgactt tgattcaata aggataattc
    11461 caaggggtgc tttgtcaaaa attgtatgaa atttgatctc actttgtttt aactctaatt
    11521 ggattttttt gtctggatca atgtctctag caatgatcat gactccgact gtttcattgt
    11581 tctcgttttt aacaggggca tattgcagaa aaaaccagtt tttttgcctt ctgtatctat
    11641 aaactctttc tatggtgaca atctttccat ccaaagcaat accgaaattt tcctcgaaat
    11701 cggctaagtc accactttct aagaaattcc tcatatccaa gtcatgatac aaatcaattc
    11761 caaaaatatt tttaatatag agttttgcaa gtaggttaaa aaattgaact ttatgcttat
    11821 gatttaaaaa caaaatagca tctgtgctat tatctaaaac agctttcata tttgcttcag
    11881 agattcgaag agtatcttct attttttttc gatctgtaat atcaataaaa ctaataacga
    11941 tttctaaaat attgttagtc ttatctaaaa taggaaagcc atttacatta acccaaacaa
    12001 tatctcttgt tctttttcga taaatcccaa aaactttatt ctgaatcggt tttttagttt
    12061 ttagaatttg atgaatagga tacaaatctt gtggacaaag ggtattatcc tccaatataa
    12121 aatgccattc gggatcacta cttttctttc ctaaaaactc atgcttgact tcacccaaaa
    12181 gttcggatgc cctctcattc atcatgagaa tggaagtgtc cggtgaatga acgacagttc
    12241 ctacatttaa attgtcaaat aacccacgat atcttgcttc gcttcgaatt aaatcgattt
    12301 caattttttt ctgagaggtt atatcgcgac acattactag aaatcttgga ctatctagat
    12361 aattttgaat ttttctagaa acagacattt caaaagattt tagattttgc ccaatctcta
    12421 aatcatattg aacaccaaat gtatgaccat gttcatttgc atggcgaatt gcattcatac
    12481 aaagaccagc tacattagga ggcaataatt cgttaacctg cttcccgata atcttatcag
    12541 ctgggaaata caataagtct tttcttttgg aatgaaatgt gaaaacttta ccttctaaat
    12601 ctacatcaaa gagaagctct ggaattgcct ctaggatact aagtagatca gcttgaacag
    12661 aaagagaagt aagttcgagc attttcattt ctgtgatgtc gattgccgtt acaatacatt
    12721 tgccattttc tgcaacgcct tctagtttta gaaattttat tttataattg gaaaccatga
    12781 ttcgaacttc acagtttttg agtgaatttg tattatgtat actttctaaa aattcatcga
    12841 agctaaagag actatcggat gtgatcagac tgcgaatatt tcgaccagtc atttcattag
    12901 aatccataga gattaaatta gaaaaatttt tatttataaa cgttatatga ccttctctat
    12961 ctaaaattag aagtggaaag ggagaaaggt catagatttc ttgaaaatgt aaatcatgct
    13021 tactgaaatt aattggattg tctgagggta aaatattttc ttcattcata agtctaacat
    13081 ttatcaagga caccgttttt ttacggctat cagccttttc taatcttcac gatttaaact
    13141 gcaagagttt taccaaatgt tttccctaag tatataaaat ttttttaaaa taattgttac
    13201 tgtagatctt ttcttcatta ttaagtaaat atactgaatt atatcacact tctacctgta
    13261 tttattctgt aaaataatta aacataacgt ttaaaaatta aacgttatgt gaattggaat
    13321 cattattaat gtattagaag gaatctatgg ctaatagaag ttatgcgatc gagaatgaat
    13381 atcaattcaa taattacttt gcgttcagtc tatttagatt ttgtaaatat ttccccctct
    13441 ctaagctaga taaatattac taatttaaaa atatcggaat caatctactc taaatagatt
    13501 tttcgcatat cgcttttctt taaaatattt cactgaaact ttttattgag tcaaaatata
    13561 gaaaactata cattgtatat tttataagtt ccttaaagat aattactatt acttagttta
    13621 aatctttctt atttgctcta ctttttcgtg aaacccggca ttcctcctac tcaccacaga
    13681 tcagaatcgg agttccgaca agatcgattc tgataagctt aaaaaccctt cttcataaag
    13741 aaaatacaac tttttccttg caaagcgtgt tcaaacattt atccttttta gaactctatt
    13801 cttgatgctt tcttccgaaa tataaacctt ctccagaata attaccccgc cccaaaagaa
    13861 acatttgcat cttttcacta ttttttatat cgaaattcct ttttttataa aaagcgcgct
    13921 cacaatctgc aaaattttcc cctatataga tagatctctt gtataattca atctccttgc
    13981 ttttgataaa aagaaatctt tgaaaatttt tttcaagaga gtcacaacta atttttcaat
    14041 ggaggatgca atggttcata gccaaaataa ttctttttca aatcgtaaaa ttgaatttcc
    14101 tagaaatgac tttcagtctt ccgatttcag ctctaccctt ttaattccaa gtcaacactt
    14161 tctcattttc caagaaaaga ttgccaagca taaaggtata cgttcctata tcgcctatct
    14221 tctcgtaaaa tatcaacttg ccatttcaaa tggtatgatt cctggctact caaaagtaac
    14281 taccaaatac caggaaaaag gacagaatct tcacaaggtt gcctttcgac cgcgaccaca
    14341 agactgggca gaattaaaac tttatagaat cagctttggt atgtctatct cagcctttat
    14401 catttactta ctcatagccg actctgttga ttttgcagaa accgtctcga aatacttaga
    14461 gagcgtcgga atttcgtcta ccccaaattt agatctatgc tctaaagtat atctttataa
    14521 cagtaagtct atctacacaa ttatttttca atatcgagag agtcgagatc attaactatt
    14581 taccaattca ctcacttgta agattaaatc atccaagcgg ataatcgcat tttgtcgcca
    14641 ctgaaaaata ccttgtttat aggtgcaaag gtcttgcgtt tgccagatgc ttattaattt
    14701 ctcaagatca tcaacatgat ccggaatcaa aataaagaaa gatctcaaat caataggaag
    14761 aaataaacga aataaacaca aagcgagttc atctagactt tctgactttt cataatttcg
    14821 cccgaattct cctgaaagat atttctcgat cgaacggtga ttaaaataac gaccgttatc
    14881 cgtaaaccca tgagcacctg gtccgaggga caagtatttt tccattgtcc aatattttag
    14941 gttatgtcta gattcttccc ctagtaatgc ataattggag acctcatatt gtcggaactc
    15001 cttttctttt agtaatgttg gtagagagtt tagaatttct atttgtaact cctcatttgg
    15061 agggagagat tctttccttt ttacttttct ataatattca gttcctttct ccatggtaag
    15121 actatagaga ctaatatgtg ttaccttttt agaaatagca ttctgtagat ccgacagaaa
    15181 catatctttt gtttgccccg gaataccata gattaaatcg ataccaaatc tctttatcgg
    15241 agaagaagat agtattctca aaacagaaga atacttatct tcatcattat aacgatctag
    15301 agtagccaac aagtttttat caaagctctg aattcctgca ttgactcgat tcactccaag
    15361 attaaataac tgaactagtt tttcttcaga aatatcttca gggttcatct ccatagtaaa
    15421 ctcagtgtta ggtgaaaatt ttaaataaga tcgaacccat tcaataaaat cttttaataa
    15481 atctaagttt gctagagagg gtgtcccacc accaataaag attgtatcga atgtataatc
    15541 tagaatggtt ggatcaattt gaattcttaa tagaaattcc tttttataat tattaaacag
    15601 aatatcttga ttaggtggat ctgttttacc aatccctact gaataaaaat cacaataaga
    15661 acatttatga atacagtagg gaaaatgtaa atataaccct gcaaagcctt ttctgtaagt
    15721 agcaatttcc aaacttaatt tctttgaatg attcttgaaa aaattagctc tgtgactgga
    15781 atatctcttg gtttatctgc attcaatgct gtatggacta acgcatgaat tgaatccttt
    15841 gttggggaaa atgaaaatag gatctttaca tttccttgat ttggcaattt gagaaaagga
    15901 gagccatagt gtaataaaat aaattgaata cttggatttt tttctgccaa gttagaaact
    15961 gcatctaatt ctttttgatc ttttgtgtca aatactaagg tttttactaa tttctttttt
    16021 aaaagctttt gcactttttt ttctgtataa taaggaatat tcaaatctat taattcgttt
    16081 tttacatttt catttttaca aacgtaagca aattttctag attcagaaat tgtaaaacta
    16141 gtaaaaggtg ttttataact tcgaatagct tctgttgata tccgagcatt caattcggga
    16201 atattagcaa tctttaaatc attgtatctt gctgtggata tttttctctt ttcggttata
    16261 aatttttcca actcttcatc ttcaattttt ttataagaat aggaagtatg aaaaagacca
    16321 ttttcgattt ttaaagcaat ctgacgtttt aagctttcat ctagcatgtt tttaccatct
    16381 acatcaaatt ctttattttt aagactttct aagatcatat tgtagtaatg tgttgtagta
    16441 tttccccaac tagtaagaag aataatatca gcacctgcta aaatcgcaag ggttcctgtt
    16501 ttttcattct gataattttt agagattgca tgcatttcca ttgcatcagt aagaattata
    16561 ccttcaaatc caagcttttt tcttagaata tctgttagaa tttttttcga aagcgttgag
    16621 ggatatttag gatctaaatt tggataaacg atatgcgcac tcatcactgc acgggcaccc
    16681 tcctgtattg atttttgaaa tggtatcaat tcaaattcca atagttgaga ctccgttttt
    16741 tcaataacag ggagacctaa atgactatct acattcgtat caccatgacc tggaaaatgt
    16801 tttatgacag ggactgctcc tcctatccta gctccttttt cataagagat agctaaatca
    16861 ccggcgtcta acgtcatacc aaaggatctt gtatttataa ccggattctc tggattatta
    16921 ttaatatcca taacaggggc taataaaaga ttaatcccaa gattatttag ctcatacgaa
    16981 gtaattaaac caacattaaa tgcataggct ggattctttg tctgccctat tgcaagcgct
    17041 cccggaaatc cagttacacc cttttcaact ctcacaactc ttccaccttc ttggtctatg
    17101 gaaataaaaa gaggtggaga gtttaaattt ttcatctcat tttgtagccc ttttgtcagt
    17161 gctcgaattt cttttttctt tcccagattc acaccaaata aaatgacacc accgggtcta
    17221 actttttgca actctgcaat agccacctca tctagataat ttttgggtat agcaatatga
    17281 atgacttgac cagctttttc ctcatcgctc atttttgata cgatttctga ggcatgctcc
    17341 aatgccctct ttcgaatttc agttaaatca gttgcagaaa tggaaaaaga tataattaga
    17401 aatggaataa tttttttgat cttcataaga gtaagaaaat atttcatgac tatttagtca
    17461 gtaatatttt aacttaaaga ttgttctagg aattgtttaa ggagaataga attatttttt
    17521 tctatatcgg atttagagtt ttaagacagg gacaaagacc atgaaataaa ttccatgatc
    17581 tctgttgtag ttttttagaa accttccaat tctctgtgag actttttatc cttctcatga
    17641 gcagcatttt gtagatagcc atccatttta tagatagatc ctaataagtt tgtatcaata
    17701 gttttgagtg ttctaagctt aacgtcagct gaaaaatctt tataattctc tgctgttgta
    17761 acacccttcg ccttttgttc tactttttct gttataatct taataccatc aaaattccca
    17821 ggagtcatat acatggttct gtaaacatat attacattgt ctctgagtga cttcttttta
    17881 tagatgaatc taacttcttt tagtttgcta ctttcaaatt catattcaat gtagcgcaca
    17941 acgctatagt tttgatgctc ttcaaaagtt gaaagagtat tttcatttac aattgtaatc
    18001 acttttctta gtctttgatc ataagttaca atcttggttt gtaatctttc aatctcggaa
    18061 actttcgcat taataaatgc ttcaaattct ttgatactat cctgcaaagc cttttccctg
    18121 agagcatccc cgggatcagc agtcataatc gatgcgttac tcgcaacaga ctttgcctga
    18181 gccaaaattc catatgtaaa gaatatacac agtgttatta aaaactttgc agccataaaa
    18241 atctcctttt attttataaa ccttctaatt gatttctatt cttatcattc tttgaaagct
    18301 gatttctttg aatgaatgta tctaatctaa aaatggaatt aaaaagagtt gtatcgattg
    18361 tcttcaacgc ttgcaatttg acttctggtg caaaatcatt ataattcact acttcttgaa
    18421 ttccttttgc cttcgtttct agtttatcaa cagttacctt tatactagac atatcatttg
    18481 gtttaaaaga tacaactttt acgattgagt gcatatcttc catcagggat ctatgtttgg
    18541 aaatcactct aatttctttt attttacttc caccatccat ctcatattca acataacggt
    18601 atacgaggta tttttgtggt cctggtatgg gatgatcctt tggttgctcg taaacaattc
    18661 gaatttcctt tttcatttca ggattaaatt ctacagcctc agcttgtaac ttttcaatat
    18721 ctgaaatttt cacaatgatc tctttttcca atgaagcaat cttgtcttgc attgccttag
    18781 cacttgcact gttcgcgact ggtttcggtt tatctgattt ttgctgaaca gctccgatac
    18841 cttctccctt gtcagaattc ttaatttgat tttgggcaaa gagcaaccca gtagtaaata
    18901 tagatataac tatataagtc ttaaatagca taaaattatc ctcgtctata aatttatcgg
    18961 caacggacaa acaaaaatat tgcctaagta aataaaagtt ttcaattctt atttattcaa
    19021 ggattaaatt cctttcttca tttaaaaaca attaaatact ccttgaccat tgattctttt
    19081 tttacagtat taaaccgtga aatcaccaaa gaattatttt atctttctca ttctgacttt
    19141 tttatgttcc attttcctta cttcttgtga gaaattggca gatgcaacgt tttcagacaa
    19201 agatgatatt gaaatgggtg ccagcttaga taaagaaata cgttcgaaac catctgaatt
    19261 ccgtatttta gaaaatccag aattagaaaa ctatgtacaa accattgtaa aacaattaat
    19321 tcgatctccc ttagtaaaaa ggaaaaaagt ttataaatac aaagtaacac tcttaaatga
    19381 tgataaagct atcaacgcat tctgcacacc cggtggttat atttatgttt atacaggctt
    19441 attaaaattt cttgaaaatg aggccagtct tgcagccgtt ttatcccacg aaattgccca
    19501 tgcagaaaaa cgacatgtta gacaaagaat gctctccgct ttaggaattg aactcatagt
    19561 ttcagcaatt ctaggaaatg gttcatccat atttcaggaa atgggcgtta agcttgctgg
    19621 cacactagca ctcctatcca acagtagagc ggatgagtta gaagcagatg agtggggttt
    19681 tttatattta ggcaatagtc cttactacca aggagcaatg tcctactttt ttgaaaaaat
    19741 cagcaaagaa gaaaagcgtt caagtgctag taaagctctc gaaaaattgc tttctaccca
    19801 tccaattcca gaagaaaggt taaaagtaaa ttcagaaaga attagaaaag caaaaattca
    19861 accaccgatt ccgtctaacc ttcttgaatc cagatacaaa aaaatgattc gaaagcacct
    19921 aggtgcagga gaaacacacg atggcgaatc aatagacgat aaaggttagt tatgatattt
    19981 gaaactgtaa atgaaatcaa tgaagccatc aaagccatca attatgcttc atacttaata
    20041 cgactttgtg cattgaatag tcaaattgca atttctagta taagttttca ctctcaaaaa
    20101 tcagcaggat ttacaccaat atcaaaggag ttaattcata ttagctcctt aattgaaaca
    20161 aaagcaaacg atttgtttca gatcatgttt aaatcgctta gaacaattac tgaatcacaa
    20221 aaaattgctc gaaaccagtc tctacaaaag aaattctttc aagattacaa caagcgctcc
    20281 gatcgaaatt atgatttaac gcatcttgaa aataaaattc tttccgatta taataataaa
    20341 agagataaat taaaggaaga ttttcagata caatttcaat cctttcaaaa gattattcgc
    20401 gagactataa aactttgtaa aaatactaga ccgatagtct tttcagtcaa gattgaatcc
    20461 gtttacattg aaggaaatga aggcgctttt gatgaattat caaaagaatt atctgatttt
    20521 atttctaaaa ttgaagattt attaaaaaga atcgaaaatt taaaccaaag actattagaa
    20581 ggaaattaat ttgaaaaaat ttcaatatat atactccgaa ggagatcata agtggggaat
    20641 tatttctcgt gatccagaga aaccgagtta tttaattgat acaaatgaat acgttatttg
    20701 caaaggaaac aagctaatta ttactgatcc aggcggaata gaaatatttc cctcagtaat
    20761 cacctctatt tcaacagaac aaaatcctat cgacatagaa tatatttttg ctagtcacca
    20821 agatcctgat atcatatctt ctttaactct ctggttagat attaatccgt ctataaaatg
    20881 tttcactagc tttctttggt caacgttctt acctcacttc ggtggaaatg atagcacatt
    20941 ttcaattata ccagacatag gttctagctt taattttcat ggtttagaac tggaattcgt
    21001 tcccgctcat tatctacatt cctccggtaa ctttcattta tatgatccaa aatctaaaat
    21061 atatttttca ggagatattg gtgctgcgct tttgcctgaa gcggtcgcaa aagaaggaaa
    21121 aatttacgta gatgactttg ataaccatat cgcctattgt aaaggctttc acgaaagatg
    21181 gatgggttca aatgaagcaa aactagattg gtgtaataga gtaagtaaaa tggatattga
    21241 acaactctgt cctcagcatg gaatgatctt caaagaaaaa gacgtaatgc gatttattaa
    21301 ctggttttca gaactcaaga ttggagtttt aagatcttaa atacttatag actgctcttc
    21361 tgtttgacga acaatttcat gaataacttg atctagttcc ctagctgatt taaaaatata
    21421 ttcaatactt ctatcttttt cttcttctgt tctatcttta tgatttagaa agagattaca
    21481 caaaccaaga atattcgcta catgttgtct aactcgatgt gattgttgcc atgcaatttc
    21541 tttaagtcta gctctttgat gtatgatttt aaattcgttt tctttttctg aggtaatttc
    21601 ttgaacgtta ttagcaatac caatcatatt cttattttca tcataaacag gaaacatact
    21661 aaaaagccac catttgattc catctgtttc ttcaataact atactctcgc ctttcttagc
    21721 tctatggaag tatcctaaaa aatcatcttt taaatattca gataaataat ctaatgcact
    21781 tgttccaatt tctaattcct tttgaaaaat tgcttgagct gttgcggcag ctactttatt
    21841 aaaaaaaaga attctcagat ccaaatccaa aaacaaggtt ccttcactgg aactattata
    21901 aatagctgtt aatatgtttt cattttgctg catttttaat tctgacattt ttttatcatc
    21961 gatcgaatat gcgacaaacg ctaccgcaat cacattttca ttttctcgta tcggtagata
    22021 agatatatct gcccaaagac tatatccata aaattgaaat tgtttttcaa aatgaacgga
    22081 atttccaagt aaggctgttt tgaaattttt agaaaagctt tctagatcag tatccatgat
    22141 aaattcaatc ataggacgag actcttttag ttcttttcct tgggttctaa aaatcatatc
    22201 aaaagctgtt ttgttaaaag ctaaaataat tccatttcga tctatgagta taaaaacttc
    22261 ctgtgtgcat tctagaattg ccttacattt taactctaaa gatagaaagg atgatgattc
    22321 attcaaattt agatatccaa taaaaatata acctatgttt ttatctttgt tttttaaata
    22381 ggagacttcg aaagaaatac tcttatatag tttgtttaat ttcactctca gatcaatgga
    22441 ttgaatctct tgcgggtcat tttttaattg ttggatcaga ttctctaatt tagcattttg
    22501 ctcgaatatt gattcaaatt ccctctggtg cttcggctcg ccaaaagtat ccttaaacaa
    22561 ataatttgaa attagaaatt ttccctgttc atctaaaata aatgcgatct gtgatttaga
    22621 atgcagtaga taatgaggga ttgcatcgag aagatcttct tgcttcataa ataaatcttt
    22681 tctgcaagaa tcatacgtca tcaatactaa tcaagaataa aacaattaaa ctcagacttc
    22741 tgtagctagt atttttactg ctattctttt atcagtctga ctagggtcct cttccgttac
    22801 aactttatag ctacttcccc attttctcat cagcccaaaa ataggtttca acttttctcc
    22861 ttttttggtt agagaatact caactcgtgg tggagtttct gaatatgctt ttctagatat
    22921 aattccgaat tcttccaaat cttttaattc tttggaaagc attttatctg atgtttttgg
    22981 aagagccttt ttcaaatcgc catagcgaag agttttattt tgcattaact taaacaaaat
    23041 tacaggcttc cattttccat tcaaaattga gagagtcaat tcaaatggac aatagtgaac
    23101 catatctttt aacttataaa cagtcatacc gttctcacta ttcggagtat ctagaatcca
    23161 tgcaatatta ttttttttat tttttccttt acaaaacctt aaaatatttt tcatgtgtaa
    23221 cataaattat tacacttact tacctagaag taagtaataa aggaaacaaa aatgagcgca
    23281 aatttaaacg ttatgaaccc tgataagaaa aaaaagatat tgattgttgc agcgaacgca
    23341 agcacatcca aaacaactgg ttggccaatt ggattttggt gggcagagtt gactcatcct
    23401 tattgggagt ttgtagaaaa aggttttgaa gtagaaatca tttcaccaaa aggtggagac
    23461 ttactaacag atggatacag tgatccagaa gatgcaagtg gatattctgc tcatgatatt
    23521 ctaagtttag gttttaaaaa atcaccgaaa catgcagaac tcataaaaaa tacaaaaagc
    23581 ataaaagatg taaaaatctc tgactacgat gcattgttta tcgcgggtgg tcaatctcct
    23641 atgtatacta tgatcgatga tactgaatta cataaattcg tcgcagattt ctatgaagct
    23701 ggtaaggttg tatctatcgt ttgtcatgga acttgtgttt tgctaaaagt taaattatca
    23761 aatggaaaat tactagtaga cggaaaaact tggacaggtt tcgcaaattc agaagaacaa
    23821 tttgctgata actttgtagg aaaacgcatt cagcctttct ggatcgaaga ggaagcaaaa
    23881 aaaataccaa atactaattt catcgtagac ggaatgttta aaccatttgc agttcgagat
    23941 ggaagactta ttactggtca acagcaatac tcaggagcag ctgctgctaa gcttgtaatt
    24001 gaagcgttag gtgtttaaat ccttaatttc ttcatttcta aaataactat gagattaaat
    24061 ttgaggtttt tattttaaat tcatttccaa agagtatatc taagaaatat gagttatatt
    24121 tattctcaaa tttaatctaa attaataact aaccaaagga gtatagtatg aaaataggaa
    24181 tcatcggaac aggcaatgta ggtggagcct tagctacaaa gtgggcacaa aaaggacatg
    24241 aaatcttttt aggagtaaaa gatacaaata gctttaaagg gaaagagcta ctttccaatc
    24301 ccaacacaaa agttctacca acacaagaag cagtaaagca agcagaagta attctcattg
    24361 ctacaccagc aacagctgcc atagaagtcg ctaagtcgtt aggtgataca acaggaaaaa
    24421 tcatcattga tgcaatgaat gttgttatgg gaaaagggcc atctggattt tccaatactg
    24481 ctgatgcaat actcgccaat acacaaacta aagatgttgt aaaatgcttt aatactactg
    24541 gattcaacaa tatggaaaat ccaatctatg gaaatgttgg aatcgatatg tttatggctg
    24601 gtagcagtga aaaaggaaaa gaaatcacta gaaagttagc acttgatgca ggctttggag
    24661 aatgttataa tgtaggtgga aacgataagt ttcaattaat ggaacagttt gcctttttct
    24721 ggatcaatct tgctatgatg caaggacttg gtcgaaacat tggatttaag cttctaaaga
    24781 gataaaatga tagttaaaaa aaatttcaat ccgattaaag ttttttctta tataaagtat
    24841 gaaatgattt atacttctat tttagcggct actgtgtatt acctgcatac gaaccttctg
    24901 actgagaaaa tatctattcc tttttccatt gcaggtctac tcggtggtgc acttgcaatt
    24961 tttcttggat ttcgaaataa tacgtcatac gctcgatggt gggaggcaag acaactctgg
    25021 tcaaatattg caagttcctc ccgaattctt gctagactca ttcttacctt cacagattcc
    25081 catagccatc aacaaaacta tgtgaaagaa agaagtgaat tatttaaaaa agaaatgatc
    25141 tacaaacaaa ttgcatgggt gcatagttta agattaaatc ttcgaaaaga aaatagttgg
    25201 gaagaaatca aacccctact cccagaatct gaatggactc aattgcagac aaaaacaaac
    25261 aaaacaggct tcattcaatt tttaatgggt caaagaattt ataaagctat ggcagacgga
    25321 acgttaggtg gttttgatag ctttcaaatg gaaggtcagc ttttatctct tagcaattac
    25381 caaagtggct gcgaaagaac aaaagacacg ccactcttaa aacaatacga tttttttact
    25441 cgggtatttt tagttgtttt tattagtgtt ttaccattta cccttctatc cgaattacaa
    25501 cggttaaatg taatttatct tttaatacca attactatct taatctcctt tgtatttacg
    25561 atagttggaa aagtaggaaa ggtaaatgag gatccatttg aaaataaaat tacagacgtt
    25621 ccactcaatt caatatgtaa ctctattgag ttagaactca gagaaatgtt agaagataaa
    25681 aatctaccta aaaaaattga acctgtcgat ggatatttaa attaattatg attcgcaaat
    25741 ataatttcta gttcttagaa ttcttttttt tgggatattc aatactatac aaagctgcga
    25801 agattgatta taaatattat aaatgaactt tatttatatt gtttaattta cttcgcagat
    25861 tctatttcag ctagctgtaa ttggaattgt aaagcgaaat ttgcttcctt tccctaattc
    25921 actctccgcc caaattcttc cctgatgacg atcaataaat tccttacaga gaataagacc
    25981 caatccagtt cctttttcgc cattcgtccc ttcggtgcta aaatgcttca catctattct
    26041 gaaaagattt tccgcaatat ttgaactcat tccaattcct gaatcttcaa cggttaattc
    26101 aactttgccg ccactttcat tcgctgagat tgttacgcta ccattttcat ctgtatattt
    26161 aatagcattt ccaattagat ttcgaataac ggtcgtgatc atctgagaat ctgcaaatgc
    26221 ttttatatca gtcgaaactt tattatttag taaaattttt ttgttagcag ctgaaggttc
    26281 aaaaagttgt atactcgatt ttacaacatc ataaatagaa atcgactccg gcttatactg
    26341 catatcccct ctctgggatc ttgcccatgt aagtaaattc tctaatagcc ctttaatttt
    26401 atttgatgca gactctaata aaattaaatc ttcatgaaac tcttcttttg aaatattttc
    26461 atattgagca gccataaaac tcagaaatga atttaaattt ccaactggac cttttaaatc
    26521 atgtgcaatg atagaaaaaa atttatcctt tgcggcaatc aaactattta gttcagtatt
    26581 tttttcagat aacttcgcaa ccaaaacact cagttcttta ttttgttcta aaataatatc
    26641 tttggatttt ttcaattcaa catgtgctct aactcttgca agtagctcta atgtattaaa
    26701 aggcttggtg acgtaatcag ccgctccaat actaaaccca tgaatcatat tgtccatttc
    26761 tgtttttgca gttaagaata taatcggtat agcttttgtg ttctcttgcg atttcaaaag
    26821 cttacatact tcaaatccat ccatcacggg catcgcgata tcaagcaaaa ttaaatcagg
    26881 cattgtatct tctacaatcg atactgcttg tttaccattc aatgcgaaac tagtatcaaa
    26941 tccttctttg cttaaaatat ttcctaagac ttgaagattc tctccaacat cgtctactat
    27001 caatatatgt gtaggacttt tttccattta tcttaaccgc tcaaatgcta tttctaattg
    27061 gtttaaagca ttcgatagtt tacctatcgc aaaagaatgt attaaatcat tcagataatt
    27121 tgcatactgt ttaaacaact ccatattaaa tgtatcacta atatgaacta aatcaattat
    27181 aaaagttcgc aatcgatttg tatttaggga ctttctagca atttcacttt ctctatacca
    27241 tacaaaatat tgacttttaa attcggtgga tattttttgt ttcagtatta atttttcaag
    27301 ctcttctata aaattatcct ccgtcaatcc ttgctgtgtt actatcgttt ccgattccaa
    27361 agttgggata aatctagcaa cttcagaaat cagcttcgct ttcgtaattg gtttcgtcaa
    27421 cactgcatcc gcaaaacctt tcatctctaa aacatcttca ttccgagcaa atgcagttaa
    27481 tattatcact ggaatttttt tgtatttttc attttgttta atgatttgtg ttgtttcctt
    27541 accacccatc tcaggcatat gaatatccat tagaatcaaa tcaaacggtt cttgataaat
    27601 catatccagt gccattcgtc cgttaaacgc ttcttttatt ttcaaatttg agttttctaa
    27661 aaagccaact acaacttgac gatttggctc gttatcttca accaataata ctcttgcttg
    27721 aacaaatttt acactcgctg tatctctgct tacttctgta tttgtaatcg caggttgatc
    27781 tgatatttca atcttaggta aaaaaattgt aaacttagtt cctttaccaa ttttactttc
    27841 taaagtaatt cgacccttca tcatctccac gagtcttttc gcaatagata gacccaaacc
    27901 agatccacca tactttaaat tactctgccc ttgctgctgt ttaaacggat caaatatcgt
    27961 tgcaatttca ttcgatgata ttccaatccc tgaatcaatc acttcaaata ttaaatcaac
    28021 catattatca tctgaattat ttttgataca tctaacgttt atacgtattc ctcctttttc
    28081 cgtaaatttg attgcgttcc caattaaatt aaacagcacc tgcctgagcc taagctcatc
    28141 cagtaaaata ctttcaggta aatgatcgtc tatagcaatt tcaaaaccta atttcttttg
    28201 gctgacttga atggaaaaca tctgtcgaat atcgctaata attgaattta gatttacagg
    28261 caaatttact atttcaagtc ttcccgcctc aatttttgca atatccaaaa tatcattgat
    28321 taaattcatt aaattgcgac cgcttttctg aatcccaacc agatattcca ataaatcagg
    28381 attcgctccg attttatctt ttaaaatttc tgcaaaccca agaattgcat tcattggcgt
    28441 tctaatttca tggctcatat ttgcaagaaa ttgactcttc aaacgattag ctacttctgc
    28501 ctcagctttt gctttctgta aattttcttc aattaacttt ctatcagtta tatctgtatg
    28561 ggtgcctacc attcgggagg gaactccttt gatagattga attacctttc cgcgtgcaag
    28621 cacccaaata taattcccat ctttcttaac aaccctatgc tcggactcgt aactaagagt
    28681 cgccccagat aaatgaagtt gaatattatc agtaacccat ccaacgtcat ctggatgaat
    28741 atgccgagtc cattcttcat aggtatccca tctatcgttc tcattatagc caagcatttc
    28801 tttccatcgc ttagaaaaaa agacttcatt cgtttccaaa ttccaatccc aaactccatc
    28861 tccactccct tctaaagcaa attgccatcg ttcttcactt acgagcaaac gacgattagc
    28921 ctcttctaat tccgccgttc ttcttagaac gctctcttca agagctgtat ttgtaaattc
    28981 taaccttttt tgaagtgaat tgaaattgat ttgattatgc actctaacaa ctaactcagc
    29041 tgcatttaga ggcttcgata taaaatccgt gcaaccaatc tccaaaatcg aaagtggatg
    29101 taaggaatct cgaagatgat taatgtatat aactggcgta tcctttgtag tatgcgctgc
    29161 ttttaatcga tctagtgtat tcctttcttg attgacctcg atatcaagta taatcaaatc
    29221 ggggtttgat tcattctggt gtatccataa ctcatcgcta gatataaaat ctaaaatata
    29281 accttctttt tgtaaaacat tttgaagtaa tattttatct tcattaaaat caatgactat
    29341 taatattctc ttctctcgtt ttaacaaatc aatgatcctc tgataaactc aaaatgataa
    29401 aatgaagttg ttctaagatt tgcaacttaa tagcaacttg cggaatagaa aaattcttta
    29461 ttaattgcaa taactccaga gaaaatccta caagcggaat aatatgattt gcctcaccga
    29521 tttcctttaa ttcttgaata taaattgtta acttattcgt ctgtattgat tttctagctg
    29581 tttctgtttc aagaaaccaa ttcttaagct ttgaaataaa atctggattg taatttttct
    29641 ctagcaatat tttctttata tcttccagaa atgtatctga atttactttt atatgaatct
    29701 ctgtttcttc tttcttagta tttggaagga aacgagctaa ttctcgaatt aactctactc
    29761 tagaaattgg cttacaaaga tatccatctg caaacgttaa gatatcatct accatctctt
    29821 tcatagcagt ggcagtcaat actagtattg gaattttttt taatctttca ttgctacgaa
    29881 ttaaaaggga tgctgtctta ccatccattt ctggcatttg catatccata agaatgatat
    29941 tcaccatctc cctctccaaa atgtctagag ccaaacgacc gtttgccgct tctaatattt
    30001 ggaaattcca ttgtgctaaa aagccagtga caactctacg attagattcg atatcctcta
    30061 ccaaaagaat cttcgctttc tcaaaagcaa tatttgaaat ttcaaaatct ttttctttag
    30121 aattatgggc aagagaagat atttctaatt ccctaataaa tacccgaaag ctggaacctg
    30181 ctccaagttt actgtctaca gtgattgatc ctcccatcat ttctactaac ttcctagtaa
    30241 tagacaatcc aagtccagac ccaccaaatc tcattgcatc ttgacctgat tgttgaataa
    30301 atggactaaa gattgagtct aattcatagt gcgcaatccc aatccctgta tctcttatct
    30361 caataactaa atctatacaa ctactctcgc ttcctcttga aatggattat aaattcctcc
    30421 tttttcagta aatttaacag catttccaat taaattaaat aaaacttgtc tgagtcttaa
    30481 ttcatcaatt agtagagatt tgggaagggt ttcgtctatc gatatttcaa attttaaatt
    30541 cttttgagca gtctgaagag agaaaatctg ctgaatatca taaataacat tgaataaatt
    30601 aactggggag taagtaattt ctaattttcc tgcttcaatt ttggccaaat ccaaaatatc
    30661 atttataaga ctgattaaat tctttccact tctttgaatc ccagagagat aatctaaaac
    30721 tactggatta tttccgattt tatcttttag aatttctgaa aaccctaaga ttgcattcat
    30781 tggagttcga atttcatgac tcatattggc aagaaattca ctcttaagac gattagcact
    30841 ttctgctccc tcttttgcgt tgagaagatt tatttcggaa agttttctat ctgttatatc
    30901 atgagacaaa acaataattc ctgtttgttt tccatcctca ataaaaggag tatatttaat
    30961 ttcaaaatat ctttcctctc catctttaaa atcaaataca ctttcaaaag ttatggactc
    31021 tcctgctaat gctttataat aattttcttc tacttgttta aatattcgat caccaactat
    31081 ctctcttaca ttcttcccca cacattcttc tgatgatgtt ttaaaatact ctgcaaatct
    31141 cttattaaca aactgaaatt ttaaattctt atctaccatt gatactatcg caggaacgtt
    31201 atctgcgagg atttttaatt tatacttatt tcgaattaac tcctcttcag caaaaatgcg
    31261 agcttcaatt aaatactgct ctctctcaat attaatagca ttttctgact ctctctgcag
    31321 ttttataact ctaattagaa taaaaaatcc agatagtaaa atcaatagta ttagacaaaa
    31381 aagacttatg attacacgat tcattgtcat ctctgattca gttacaaaga taactacttt
    31441 atttcgagca aaaccaatta tataatttgt taattcgtta atcggtaggt atatttcttc
    31501 ttctacacga tttctaagtt cagcggcttt tcctactctt ccagaaataa gatattcaaa
    31561 ttcttcttct ctcatctgct tccaattact cagcaatcta tctatctcgt ctactttgga
    31621 ttgatctccc aaaaaatttt cacgtatcga ctctagattt ttttgaactg tgttttcaat
    31681 ctcattcacc tcccttgata gattttcaat ttcattctta tttgaagaaa agaaactctt
    31741 tagcataatg tttcgaattc gagcaacgtt atatcggagt tcaatggctg cgttacttac
    31801 agtaaagggg tgaatatata tatctttcgc aatagaatct aagcgaagaa tagcaggaac
    31861 agctatgaga gcgattacaa ataaacaaaa aaatattaca atataccaaa aggaaattgt
    31921 tttaacaatt ttggattgat ttcttccttt taaaaaatcc aacataatat ccactttttc
    31981 tgatacattt ttaattgtaa aaaagtattt cttttctcta agagaattaa acatgcagaa
    32041 aacaatttac agaaatttga aagatttaag aattgagcga ataggagaat cttaatgggc
    32101 tcagaaaatg aagaaaagat agatgatgag aaaaaagaga agaaagataa aaaaaaacca
    32161 aaacgtttta cttccgaagg agaaggtagc tctaaacttt ctagcaaaaa gtatgaaaaa
    32221 catttagcaa aacttcagct cgagttagta aaaatgcaat actgggtgaa atatgctgga
    32281 gcaaaaattg tgattatatt cgaaggtaga gatgctgcag gcaaaggagg aacgataaaa
    32341 cgtatagttg aaccgttaaa cccaagaggg tgtagggtcg tagccttagg aacaccgagc
    32401 gatcgagaaa aaacacaatg gtattttcaa cgttatattc cacatctacc agctgcaggt
    32461 gaaatagtaa tatttgatag aagttggtat aatagagcag gcgttgaaca tgtaatggga
    32521 ttctgcacag aggaacaata tagagatttt atgcaagatt gtccagagtt tgaaagaatg
    32581 ttagttcgtt ctggaattat acttttaaaa tattggttct cagtgagcga tgatgagcag
    32641 gaaagaagat ttcaatccag aacaacagat ccggctagaa gatggaagct aagtccaatg
    32701 gatttagagt ccagagataa atgggtagag tattcgaagg cgaaagatgc aatgttttct
    32761 catacaaata ttccggaagc accttggttc actgtagagg cagatgataa gaagagagct
    32821 agattaaact gccttcatca cttacttagt aaaattcctt acttagatat gactccagac
    32881 ccattgattt tacctccacg taaatcagcc gatgattatg tgagacctcc gcgcaacgaa
    32941 cagttcttcg tgccagaggt ttattaactt gatctcgagg ttggattttt tacaccacca
    33001 ataaacatgt gatggcgtta gtcgaaagat attctagcaa aggagaaggc tcaacaccga
    33061 tatttttaat attcttaaat tttatcggtg gcagtctttc aattttctag tgcatcaaac
    33121 taacatcaat ttagaattag ctctttattt tcttcgagga tttttagaac ctggaacaca
    33181 gagatttcgt tttgattttt cgataaagtc agcaatctct gttgcaaact tatcatttgg
    33241 aaatttttct ctcagtggtt gaattctatt ttccatagcc ccagtcatat cataaagaaa
    33301 ttcataaatc tccctgattc tatggatatc atcctttgta aaacctttcc gtttgagacc
    33361 tatgatattg agccccgaaa attcatttgg tctttcgtgt tcggataaag caaatggagg
    33421 aacatctttg gtaatagccg caactccagc gatcattgca tatcttccaa ttctgcaaaa
    33481 ttggtgaaat ccagacttag cacctaacac cgcatagtct tgaatttctg tatgtcctgc
    33541 gagaccaaca ctcgcaagaa ctacaaaatt tccaacatgg caatcatgtc caatatgagt
    33601 ttcagccatt ataaaattat tatttcctat gactgtttca tgtccgggtg tgcttgcatg
    33661 aatgctacac ttttctcgga taatattatt ttgcccaatg tttaaaattc catcttcata
    33721 atctgtatat tttaagtctt gtcctggagt tccaagtaca ttgatcggat aaatagtatt
    33781 gccctccgca atagtagttt ttccagctat aattacattt tgcccaattc ttacattgtc
    33841 cttgattgta accttaggac ctataaacga ataagctcct attgatacgt tatttcctat
    33901 cgttgctcct gattcaatca cagcagtagg gtgaattcct ggcttaaata gtattgatga
    33961 catagaatcc tctttttcct aaattcgcct tttgaaaaaa ggcttttatc gatataaaaa
    34021 aacgaaagat actatagtct aggtttattt tttgaaaaat ttaaaaatct tacaaagaat
    34081 caactttttg gatttaaagt atagagaaaa aataccagaa tgattgaaat aatactagca
    34141 ccaatcataa gacctctgcc agctttccaa tttccaaatc ctgaaaaata taattgatag
    34201 gatcgagaaa gtataaagct agatttctaa cagacaagaa aaggaattcg tctagcctcc
    34261 taagtaaaaa ctaaatatca attgattcga ttctaaaaat aactgctttg tataaatttt
    34321 tctagaatca gaccaaaaga atccaacaaa gaataatata gtaaggaaaa agctgtttag
    34381 aattaaggtc ggcgcgaaaa cttgatttcc cttaaatgag ctttttaact cacctaacac
    34441 tgagcataag gtgagtgatt cactttcaaa caaagctcta attttaaaat tttatattct
    34501 tactccaatc caaaaggcgc tgcaaccagg acttttctaa tttttctgaa cgagctttga
    34561 taacagattt tcgtttttcc ttatcattta taatttcttt ttcgattgga gttaactctt
    34621 catattcttt aagtttttga gtcatactag cctgatctat atcggatgtc gtattgttta
    34681 aatcttcctt ctctagtttt tctaacttct cctcaactgc ctttggatcg acatctttat
    34741 ctacctcaga cgcagaatct tttttgatca ctgtttctag tccagtatct tttaaaattt
    34801 ttgtagatac tgattttgga acctgagaag attttccact ttcaattaca atctctttag
    34861 actcaagtgc atcatcgacg gctgagagag ttttactttg cttgatctct gttctagaat
    34921 atttttccaa ttcaggaact ctcactttca tggagaccga accttcgacc acgtttactt
    34981 ttgaatttcc gtttggcgca acgtttaaat catacttggt tccacgaact cccgctgtga
    35041 gagtgggcgt tgaggcattg acttcttcat ttctcccaag cttagataca ttcaacatag
    35101 cagaccctga atctacaata aaattgtttg tctttgtaaa tccaacctgg actcccttga
    35161 gtttaaattt ggaatattct ttaatacgaa tgacagctaa cgtctctgtt ccaatcattt
    35221 gcaaatcaca gacagacttt ccgccaacca taactagatc tgtatccgta atttcatcgc
    35281 ctaaagctag tgattttgca gtagttctaa catctcccgt cctaaaaata acaactgctc
    35341 ttggatggta ttctttttcc ttaccagaac aatttcccaa aatgataaat attataatta
    35401 aaataattct taacaaattc tgctcctaat caataattca tatagattcc tacctgttat
    35461 gaaaacagat ttgtaatttt tgaaaagaaa ataaataaat cataatcgtc ccattactac
    35521 tctatggtgt taaattttat tttttttcta ttagatctaa ccgttccaca aataaaaaaa
    35581 gatttataga aaatccaaga agtaaacaaa gactttttgt tcaacctaaa caattctatt
    35641 gaattaaaag gaaatcggtt ctcaatcttt gaaaaatttc ttaatagaac taaatttagt
    35701 tctatgctaa attgatacgt taacagaaaa agatattacg atttctgagt ttctcaaatc
    35761 agaaatttta attataaata taccatcttt tgagttaaag atttgtgccc tatgattact
    35821 ctaaaattaa taacagagga gagatagatg aaatataatt tatttttttt atgcatacta
    35881 acgctttttt actgcaatac gcctccaaag agggaagcga ctaaaaatcc gaaagaagaa
    35941 tatgaccatt gctatattaa atctttttat ccagcggaat ataaaacaat agaagataga
    36001 attctaattg cccccgcctc tataaaagaa gaaaccattc ctgcgcttat aaagacagtc
    36061 gaggaaactg tcattgataa acctggtaaa tcagtctggg tcgcaaaaga gaatgataga
    36121 tattgttttg aagaaattcc acctacatat aaaaccataa aaagagatgt tctcatctct
    36181 cccgaaacta caaaaatggt agaacatcct gatgaataca aggttgtgac ccgtaaagag
    36241 ctcttgcaag aagctagagt agaatggacc gaaattctat gtgaaaataa atacactcca
    36301 gagcttataa aaaaaatcca aagaagtcta caagccaaag ggtatccaac gggcagagaa
    36361 gccgagttag ataatgaaac ttttacagct ataaataaat ttcaaaaagc aaatcaatta
    36421 aaagtaaata aaacaaaatc catctacctc gaaacaatac aagcccttgg agtaaaatga
    36481 agtttattta cgaactgata gaacacaaac ctattcattt gagaatagta agatggaatt
    36541 gaaaatatta gagaatggaa gaccttagac caaacgccaa aagaaaagtc ccattttaaa
    36601 aataaaaaga tgcatttggt ctaaacacat attagtttta ccgcacgtct gtatgtgcgg
    36661 tataccaaat actactcaaa taaaatcctt gtgggaaatt acttttgtca tttggtatat
    36721 attctacaag atctatcctt tttaaaatgg ataaagcgat aagtaaataa aatctctttg
    36781 gaatgctgaa tctactaatc tactttaatg agtttgttca agagtaaaaa gccaaaatga
    36841 tcatgtattt tactaactgt ccttacgcaa aagatagaaa tcaataagta ggataaagga
    36901 aattagaata ttagataaag cgaatattca atagcctccg gtatagctcg gctgatgggg
    36961 agcgaaaagc gatgggagga aagaaagccg ttaggcgcta atttcatttg aacgtttggt
    37021 tcaccaaaac tcaatcattg cttttgttaa gcaatgataa tataaggtga gttactaatt
    37081 tatttcagaa agaaaaatat ctattctgcg atatatactg aatatgcaga agatttagtt
    37141 ttatcagctc ttttggatag gttttcagac catagtattt tcccagactg atctctaact
    37201 tttataattc catcactcga aagataagta ttattatcaa ttggaaactc tgattgattc
    37261 acttctactc cttccatttt tttaaactga tcaagattgt tacttactat gtataaattt
    37321 ttttgtgtct tcatatagat atattttttg taaatataaa gagttgcttt ttcgtctaac
    37381 tttccactat tcaagaattc accttttgca ttgaagcgat aaattgtatt ttctctaagt
    37441 aaataacaag aatcatcaat ggttccaata atgcgaatct ctgcctccgc actcaaaaga
    37501 gtaaatgatt ctgtcaatgg ctgtaattta gagtcataga tttcccaact cattccatca
    37561 gaattattat ggaatatata aagctttccc gtaatcgtta attcccattt gctctttggt
    37621 gggattgaaa tcgatgatag aaattcccca tccatagaat aagaatataa agtttctact
    37681 cccgcaattt tttctgaata tagaatagta tcactgatct gaaagcggat aatagcattt
    37741 tttctcatag gaatactcca aagaagtttc cctgtttcca agtcaaattg atctagggaa
    37801 gatttagaaa gaccatagag ccttgtattt gccttcaaaa ttctatatga cgatggagtt
    37861 tcaattcgcc aaagtattga ttttttttca gaatgaattc gagtaatata ggttccataa
    37921 tttacaatca gtgattgatt gatatagatt ggttgagact ggatcatatc ttctaacgga
    37981 taactttgta tagaaacttt ttctgatcta tctcctattg tttcagtgga aagatttttt
    38041 gcataggaag attgcggaaa attatcgatc aattctttct ttaatgactg aatcgatttt
    38101 gtaattttag tcttatcttt ttcattttgt aattctttta aaatttggat atatgcgtat
    38161 aaactggctg catttctatc tatagagagg cttttcaaaa tatatttttc tgctttcgaa
    38221 aaatcttttc tctcaaagta gatataactt agatgataga atggttcctg aaaatttgga
    38281 tactttttgg tcaggtctaa aaagatcagt tctgcatctt ggattagatt atcattataa
    38341 agtaagatcc ctaagttata cgatgctatt tctaaatcag actcaagact taacgcataa
    38401 cgaaaatctt cccctgctct ctctcgattg cctaatttgt aataatttat tcctcttgct
    38461 atatataaat ttgaatcgtg tctttctata gcaattgcct tttcaaaata tgataaagaa
    38521 agtcttctat ctttttctag actattcaaa cctagttgat agtaacttcg atacgattcc
    38581 ggattctttt ccaaaatctt tttatataaa ccaattcctg ttgttatttg atttaatttt
    38641 aaatagtaaa aagcaatctg ttccagtaaa tcggtgttat cctcactttc cctctctgct
    38701 tttttaagaa aagaaagaca agcttccttt tgattctgca gtagatatag atttattatt
    38761 ttatttaatt tctcaatcga cttctcttta aaaaaggaag atgagtattc tgctatagct
    38821 aaatcatttt cttttgaatt actaagtttt tc
//
LOCUS       DAOQOS010000046        37742 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_446_length_37742_cov_6.911534, whole genome shotgun sequence.
ACCESSION   DAOQOS010000046 DAOQOS010000000
VERSION     DAOQOS010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 37742)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 37742)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37742
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_446_length_37742_cov_6.911534"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(648..1106)
                     /locus_tag="PLF66_11785"
     CDS             complement(648..1106)
                     /locus_tag="PLF66_11785"
                     /inference="COORDINATES: protein motif:HMM:NF025677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase zinc-binding domain-containing
                     protein"
                     /protein_id="HMZ64770.1"
                     /translation="MYVCNTDKAKKPNYIPSLERASQYKSIWKKHWEEFLRIYDMKFL
                     SKYGELSEGKIEEVEKLLGCGDFLNGFQRYSCEECDVNLIVPFSCKSRLCLSCYRKKL
                     FGWSVNLSHILNIDLRHIHVTFTIPVVRYARSDSATHQPDMIVYARYSSR"
     gene            1970..2470
                     /gene="flgN"
                     /locus_tag="PLF66_11790"
     CDS             1970..2470
                     /gene="flgN"
                     /locus_tag="PLF66_11790"
                     /inference="COORDINATES: protein motif:HMM:NF016985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FlgN"
                     /protein_id="HMZ64771.1"
                     /translation="MKTNLELLEKTFKEEIGIYKNLLILETSKKEAILRSNGKHLENF
                     TKQTSTIMENLATVEAKRMKLVADFFSDEKNGAVVPTVTEFLNRLDKVTLAKFKPITN
                     ELKFVVTDLKEKLTVNEELLKSKLDIFALSIDALKNAGEAQVSDLYGDNSKSKVRTNV
                     MLNMRA"
     gene            2480..4387
                     /gene="flgK"
                     /locus_tag="PLF66_11795"
     CDS             2480..4387
                     /gene="flgK"
                     /locus_tag="PLF66_11795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004765678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HMZ64772.1"
                     /translation="MGSTFSGLEVGKKGLSVHQQALHTTGHNISNADNKQYSRQRVQI
                     TSAEPLYDPAYNRALVAGQIGQGSKVPEIERIRDSFIDDRIQETSSIKDYWSAKNDYL
                     YRIEMIMNEPGGMSLRSQMDQFWAAWQELANYPDESAHRSVVKEKAVGLGSRIEDTYR
                     KLTQLQEQANREVESKTNQLNVLGEGIRALNERITKAEALGDRPNDLYDRRDGMLQEL
                     SELVDISIGRSDKDEFMVFIGQQIFVQGIKKESVELVGNPENDGKFKLIWARDGKDVL
                     LRSGHLQGLIEVRDFVLHEKIDNVDTFAINLTDTVNEIHKDGFGLNGKTNINFFQPRD
                     LSLNSNAEYDSDGDGINDKTAIYRVTGKMTLDPARPISISGNITLHKNDEKSTPVIIP
                     YNVDDTLNDVIKRINRSESGVVAFMNHDNQLVIKAVTASNSRENFMIRHLEDSGNLLV
                     GMTGILNGSGVAGSFDYNRLGEINKFQSNPSDITLTPQFHPASFVRVSDEIANNVNSI
                     AAGRGKDVGGTGDYNKANGQKDGNNALLIASFLKDKPMMVNYDQNFTEFYNGMISKIG
                     TEAREAKQELGTQTALLREFEKLRQSVMGVNLDEEMANMVQFQHSYNAAAKMINVQNE
                     MLDVIINRLGV"
     gene            4390..5655
                     /locus_tag="PLF66_11800"
     CDS             4390..5655
                     /locus_tag="PLF66_11800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001223328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein 3"
                     /protein_id="HMZ64773.1"
                     /translation="MRITNQISNNTLINTLARHQSQLDETQNQLGTGLKLSRPSDEPS
                     AVANNMYFRTRVNELDQFMKNAGEGKARLQLIDGQLERVTEILQRVRELTVQASNGIY
                     QGDKGFELEVAIGKEVNELLKATVDIANTRDATGRYLFGGHTIDRPPFESVEAKMKGL
                     KGIEVQDQIIGVEYRGDIGDQLREIERGEYVGVNVAGNKAFWGTNMSITANRDSSSYS
                     ATSDQKFKIDGTEISVSVGDTLDDIIDKINSASIDVKASKLGSDNITLSSTAPHQIWL
                     EDVDSGTVLRDIGLIEVNYAEPPSNYSKEATVVGNSIFDVMIQLRNDLTAKDQERISG
                     RDLADIDMALENILRHRSIVGAKMNRLEEHEKRIDFDKTFMAELLAKNEGIDFPETIM
                     NLKWLETIHQSALSVGSKVIKPTLMDFLR"
     gene            5657..6109
                     /gene="fliW"
                     /locus_tag="PLF66_11805"
     CDS             5657..6109
                     /gene="fliW"
                     /locus_tag="PLF66_11805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010573183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliW"
                     /protein_id="HMZ64774.1"
                     /translation="MTKEIQTKPFGAMKIDERQILSFPEGILGFEKYNEFALIEENEE
                     SPFKWLQSLSELDLAFIVIQPDLFAPDYKPVIGQEDLETIQLSQIEEGVVMTIVTIPN
                     DNPRMMTANLQGPVIINPKNRMAKQCISKNENHPLRKLILENVPMESV"
     gene            6109..6348
                     /gene="csrA"
                     /locus_tag="PLF66_11810"
     CDS             6109..6348
                     /gene="csrA"
                     /locus_tag="PLF66_11810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012390131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HMZ64775.1"
                     /translation="MLVLARRLNQSIIIGNDIEVVIVDIKGDQVKIGIKAPKNVSVHR
                     AEVHQEIQAENIQAVNVSIKPEELGKLSNILKKKK"
     gene            6498..7028
                     /locus_tag="PLF66_11815"
     CDS             6498..7028
                     /locus_tag="PLF66_11815"
                     /inference="COORDINATES: protein motif:HMM:NF019235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1564 family protein"
                     /protein_id="HMZ64776.1"
                     /translation="MQSKQYNGKTISKPKICYPNANLKLSDFSSTLLIPNKYLTLFQT
                     KLMQHKGVARYIAYLLSKYQIFIANGMIPGYSNVTTKYQEKGQNLQKVSFRPRPEDWA
                     ELKLYRVSFGMSISAFLVYLLIADSVELAATVSNYLETVGISAIPNFHLSAKVYLWNQ
                     KTYYSTVFQYRKSHYH"
     gene            complement(7061..8032)
                     /locus_tag="PLF66_11820"
     CDS             complement(7061..8032)
                     /locus_tag="PLF66_11820"
                     /inference="COORDINATES: protein motif:HMM:NF014075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF115 domain-containing protein"
                     /protein_id="HMZ64777.1"
                     /translation="MYSEEYSSFRDKITAKLGVNASTISYFARTWLRNYFINLQLCSD
                     RDNTGLIFKDSLPIQNKPVLYIGAAPNLEDQLEWIKKNQDDFFLLAADTACYLLLQNG
                     IRPDYILSIDSGRGTIYHFRNIPEDIPIISWLGGNRYIFSLKNPILLCLTSYPLDQVL
                     HSVVFTSSNEIIQNPSLNVAGLAKALAEKFLAKSLSLSGISFKKENGKTHSRGTGYES
                     FHVPKVNRYYPMESYIPHSYQKDISSKNQKALYHLTDTTKVPILKLDTHLPYLKDSSI
                     YKTKPLSLKINQKQINQFLHLLKNPNIQKEILNELGVEKSVLLRFIF"
     gene            complement(8471..8959)
                     /locus_tag="PLF66_11825"
     CDS             complement(8471..8959)
                     /locus_tag="PLF66_11825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002741605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HMZ64778.1"
                     /translation="MSTQEFEIHKLIELARTKKYELAVAGFAALDKIDRVPVPKKLKT
                     RKIAVQALYALSDGLVKYDYFTAEQRKKLQAEANMSDAPYKGFKGLFSNSAAPVVEED
                     IEEDYIPQEEEAKHDFVAEGDSDELDSASDDEDDNDDDDLDDEDDDDLDDDDDDLDDE
                     DD"
     gene            9148..9723
                     /locus_tag="PLF66_11830"
     CDS             9148..9723
                     /locus_tag="PLF66_11830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HMZ64779.1"
                     /translation="MKQFIPFLFILFLLSCYSQKQSNFDVSLYEGLDLSGKQIVLKDL
                     SYKRLAINVYAPTCVPCVKEIPALNAIYKDISKDKSLGLFMVVDPYDIVPDAEPSNTF
                     EQLYPKAKEIMEKEVIEKKIELPILIMKRPFKVSQGDGLITGRPETLLFKTNPLILYY
                     NFIGPLSEEKDISIIQNQNKIKFFKLMLGGV"
     gene            9723..10421
                     /locus_tag="PLF66_11835"
     CDS             9723..10421
                     /locus_tag="PLF66_11835"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012389787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HMZ64780.1"
                     /translation="MRSLIVRFLDCEGPGILESSLRNANYKITYHDAYKKGIQLVPES
                     QQIFDIIVLMGGPQAVYDPKLESFFYPYLKLVENALSIPGKKVLGICLGSQIIAKALG
                     SKVEKGKNGPEVGFSNVSVSNSSHPIFSGISSKQLTTFHLHGDTFDLPQGSERLLASE
                     KYENQMFSYKDKAIGIQCHFEVTYPMLEVWWNIHKEIPSTLGKISPEIKTKQQEMEAN
                     ARILFDNILKLKAN"
     gene            10445..13186
                     /gene="secA"
                     /locus_tag="PLF66_11840"
     CDS             10445..13186
                     /gene="secA"
                     /locus_tag="PLF66_11840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020780757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HMZ64781.1"
                     /translation="MFQKVLQFIFGSKYERDLKRLTPIVAQINALEPEIQKLSNDELK
                     NQTQKFKNLLASGKTLDDILPEAFATIREVSVRTMGLRHYDVQMMGGIALHWGNIAEM
                     KTGEGKTLTSTLAVYLNSLAGKGVHVVTVNDYLARRDALWMKPIYDFLGVTVGIIQHD
                     MEHEERQLAYGSDITYGTNNEYGFDYLRDNMVPRKEHKVQREHFFAIVDEVDSILIDE
                     ARTPLIISGAADESTDNYIKIDTIIPKLVEKDDYEVDEKARNVLLTEAGVKKVEQIIG
                     VDNLYSPDNVELVHHVHQALKAHKIFQRDVDYVVQNGEVLIVDEFTGRLMPGRRYSDG
                     LHQALEAKEKVTIARESQTLASITFQNYFRIYEKLSGMTGTADTEAEEFAKIYKLDVI
                     VIPPNIKMKRIDHPDRVYRTEREKFNAIINEIKELHAKKQPVLVGTISIEKSELLSNR
                     LRMAGLPHNVLNAKFHEKEAEIIANAGKPGAITIATNMAGRGTDIVLGGAQKFKDLME
                     KWTEVDPQIDEFKLAIKKKDYDTAKQIAESFSQHGKKKKAKEIIDEVLIWEKAHEEVL
                     NNGGLHILGTERHEARRIDNQLRGRSGRQGDPGSSRFYLSLEDDLMRIFGSDRISSIM
                     QRLGMEEGQEIEHKMVSNAIARSQKRVEGHNFDIRKHLLEYDDVMNAQRIFIYNLRND
                     VLNEKANMEELIQDWVKEVVDLQIEAHCQGKNPAAWDYISMNEWLTTMNVSVRIQDGE
                     FSRSSEVQERLREKLVTEFVNVYKGKISKVGKEVWQYLERSILLDILDHRWKEHLYVM
                     DHLRDGIWTVGYGEKNPLVEYKIQGFKIFATMVDNLKAEVIGFLMKVEVAEDTKIEEK
                     EIEYRKVGEESTDIDLFNMNGNRNDLIQTTNKRKIDTNVTSGSSGGSSSRKSSRRKR"
     gene            13187..14059
                     /locus_tag="PLF66_11845"
     CDS             13187..14059
                     /locus_tag="PLF66_11845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012476089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HMZ64782.1"
                     /translation="MLLNEILINTKTGVSSCPYCKGVGIVLDEVIPGSRTGSLSLCSC
                     VTDHCIPCPAKAKAPYRIYEESMDKMIPCFCQEARLTRNRLHYLIESSNIPPRYRYRF
                     LNSMDVSGNNMISLFVAHDWANELILNWNNPTHWKDKPKQGMYLSGGTGSGKTLLACI
                     ILNELIFRYGVKCRYAKISKDFLNALKDTYQKDSEYHGQERTIEMEFAEIDVLVIDDF
                     GVQKDSDWVNAKLYDLIDSRYEKEKITLLTSNLPLYDWKEKGEGRVYSRLCEMTHEIK
                     LECPDYRLKFQMST"
     gene            14066..14476
                     /gene="folK"
                     /locus_tag="PLF66_11850"
     CDS             14066..14476
                     /gene="folK"
                     /locus_tag="PLF66_11850"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010571226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HMZ64783.1"
                     /translation="MSNNLKHTAFISLGSNLGDRNLFLRKAIQAIEEIPCKIIQHSKI
                     IETKALDFIDQPDFLNCILVIKIDLSPIELLDALQSIENKLGRIKRFDKGPREIDLDI
                     LTYDNLIFKNERLVLPHHSIKTRPFIKELLEDLK"
     gene            complement(14709..16181)
                     /locus_tag="PLF66_11855"
     CDS             complement(14709..16181)
                     /locus_tag="PLF66_11855"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HMZ64784.1"
                     /translation="MDKQSENHKEEIIKALRKELVSSEIRFQKIITHNSIPILIVGHD
                     GMIRFANPAAEYLFHKMKERLINMPFGYPILQGEVAEIVIHAKNQPDSIAEMRVVETI
                     WENEPVFLISLRDVTERRNMMERLRVEEERYRSLVELSPFGIYVHLDGVCVFANTAGI
                     ELLGGKNPEDIIGQSVMRFVHPDYRDLVVDRIGSILTGDKHLLPIEEKFIRLDGVVID
                     VEVSGIRFDYMGKVAIQLVVIDITERKKSEENVYKLNSELQRSFSQKTKELDLMHLEL
                     DSLQSIIASDDNAPLKSIHDSTEYLKEKLVDKIDSNEFKHLENIQKQTANASYLIESL
                     LILTNITDANLDMETIDITSICRNLMNEIEKLYSEQNLEFSIQGNLQLFGNRKLIRIV
                     LYNLLDNAIKFSKNKPIVKIEVGSIQKEGKNIFFVKDNGVGFHSESERIIFRPFQKLE
                     PSEGIGMGLTIVNHIIEKHNGKVWAESEIGVGSTFYFFIE"
     gene            complement(16174..16455)
                     /locus_tag="PLF66_11860"
     CDS             complement(16174..16455)
                     /locus_tag="PLF66_11860"
                     /inference="COORDINATES: protein motif:HMM:NF019309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0048511 - rhythmic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="circadian clock KaiB family protein"
                     /protein_id="HMZ64785.1"
                     /translation="MEKLAFYLYVSGKAQSSEIAIQTVKSFIAEEVGSDCDLFIFDIS
                     EDPYLAEAAKILVTPTLIKENSEKSIRVVGNFNNRRKLLESFELEKVYG"
     gene            complement(16516..17088)
                     /locus_tag="PLF66_11865"
     CDS             complement(16516..17088)
                     /locus_tag="PLF66_11865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64786.1"
                     /translation="MFRFLCIIGFAVFLLVGCKEKNGNIGNSNPNLTTEEKKSEFIKA
                     QISWAYIDLPLEMKIYEIKSQQSYELWETRSVPSKDSSPASIEIPNSTIVIQSGRYKE
                     FILGMQNQTDKPIHFFAAPHSVDPPQYSLGFKFKCLCINHAFTVKPKEFWYRVVRLNI
                     DKEFRGDSFTIKHDLIGISEQRMKDFEFKP"
     gene            complement(17095..17760)
                     /locus_tag="PLF66_11870"
     CDS             complement(17095..17760)
                     /locus_tag="PLF66_11870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64787.1"
                     /translation="MSPAKDFLSILQNDYNNFEKYFKSLLKNYSRVFDFIERVIQIIP
                     LEVFVLLFFSLIILILINSVSPSTSKINFLFAVLASVGVYFIIAKVGVLNKKRFSDTM
                     DRTFISALFLLIPVYSFYFLTLIYKYFTGVYRKRKIASPVSMEKSIFHVQSAYNDLMS
                     EYYSLSENNFDTSQLKEKIQILQIASDGLLNLLNPVPKPMANVNHVESIESISDPEVV
                     TTK"
     gene            17762..18799
                     /locus_tag="PLF66_11875"
     CDS             17762..18799
                     /locus_tag="PLF66_11875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64788.1"
                     /translation="MKTPVFLINIFGRVSKKYNSLSFLTKIISIYTLLAIPFLYAHES
                     HLPWFVILILILTTLFVSLSTLFLSILILKKLFQKDFILWQEALYTVSVILIVYSFGF
                     FENQAGVIFLLFVLLFFLFYRAVRVVVYYRIILVGMAIFVNGLLTFKYLQFSEIYLFN
                     RSIQTKYNEHEMDLSQWSFSEETRILKNETLRLTLKLPKDFFFHNPKDLKMKEKTGTG
                     QIAGILSSSQVDPNRYPLVRIFFVPEFIKVDSTTIREEYSSILDFEKHQGNMEEIKNL
                     GERLHPERKWQGNFWTFYDIIRPRYSKTGFYIYPLKEGSYLILDITENLVDKQFHENE
                     IEEIITSIQQN"
     gene            complement(18796..19533)
                     /locus_tag="PLF66_11880"
     CDS             complement(18796..19533)
                     /locus_tag="PLF66_11880"
                     /inference="COORDINATES: protein motif:HMM:NF040144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5683 domain-containing protein"
                     /protein_id="HMZ64789.1"
                     /translation="MKLKIFLYLFILLLSSAVFSETIIMRNGNVKKGRIVSQDKLSIV
                     FEEKDAKDPEVFSKKEVLKVSYKDLTQQEINQILKENGGAEMVDVSPGTPVLDPVGGR
                     RSITLGKAFLRSLVLPGWGQMYQNRQIAATAYFILFLGGLGITGYQYKEYKSESDDYN
                     YKAKNYMFALYYRDSFTQNLARADMTAQKAVLDRSLHRLEISAGILAGVYLLNLFDVV
                     IFRPKDNLSLRASAFTDTLSVKLEYQF"
     gene            complement(19740..20351)
                     /locus_tag="PLF66_11885"
     CDS             complement(19740..20351)
                     /locus_tag="PLF66_11885"
                     /inference="COORDINATES: protein motif:HMM:NF017945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate hydroxylase PuuD"
                     /protein_id="HMZ64790.1"
                     /translation="MEDNNGLLLYAVRWIHYVSGVCWIGLLYFLNLVNVPLQGKLDAG
                     VKKVLVPELMPRVLFWFRWAAMFTFLSGLTYYVLFFEMGGVRAATPNWSVWILLGMLL
                     GSIMWFNVWFVIWPNQRKIINGVKSGNPADPSIPKKALLFSRINTFLSVPMLFFMGYG
                     RHAGQHVSIASGEGIMVVILAGAVGAGVAWFAIKQSGSISTEV"
     gene            complement(20464..20991)
                     /locus_tag="PLF66_11890"
     CDS             complement(20464..20991)
                     /locus_tag="PLF66_11890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004488043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HMZ64791.1"
                     /translation="MKKILIILFLGFIGNIYAVSIYDFKVKDIKGNEVNLSSYKGSPV
                     LIVNVASKCGYTYQYEGLEKIYKKYKDRGFKIIGFPANNFGAQEPGSNDEIAKFCKLK
                     FDVSFDMMGKISVKGEDKHILYKYLTENSDKKGEIGWNFEKFLIDKNGKIVDRFESSV
                     EPNSEKILKRIESIL"
     gene            21309..21902
                     /locus_tag="PLF66_11895"
     CDS             21309..21902
                     /locus_tag="PLF66_11895"
                     /inference="COORDINATES: protein motif:HMM:NF018184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HMZ64792.1"
                     /translation="MKHFSMFLFAFIIFTQCMPSPDLAVYQSLKDPKISTKSSIKVLT
                     ITLTGDPSQTSGEAFGKLYKLKYKLKNDSLKEAWPRARWPKPFETPRNEYVGIFALPV
                     SNEVDSIPENIENPEPKVQLETWNYGSVAEILHIGSYQNENPTVEKLKKFISENDYEI
                     IGFHEEEYLKGPGMFFKGNPDEYQTIIRYPVKKLEKK"
     gene            21920..22822
                     /locus_tag="PLF66_11900"
     CDS             21920..22822
                     /locus_tag="PLF66_11900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64793.1"
                     /translation="MFSYPFILSLFLFLLVFNASLFSQATETKEKPAKKDLFTEEDEN
                     SPAVERFRKREFELGVRFGLGYHAEDRFESQLRNYKSESSPYILTSVYLSPIRSTTNT
                     EILARIRFFDNGKAGFVYGNTQFKPFHLSEVTSLNFTNFYTASRLNFNLKVEYFFLTY
                     THEFKFRNFYLEGGMGLGINTISWKTNGDTISRFEYTPQSGFMVGNGLGYKLEGSFNK
                     KVTDNWILQIGSFYNYYTVPSFSGQFNDETSSFYLRSDGSIAPLSTAQFRDSIVDTNY
                     ASRQLDMRAGNLVLFLSLIFRFSL"
     gene            23699..24775
                     /locus_tag="PLF66_11905"
     CDS             23699..24775
                     /locus_tag="PLF66_11905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64794.1"
                     /translation="MIYWLSIILSLIFHFNCSGEGKSEKIKPNYLTLSYANSKSGYET
                     ANLSIADHNKLQNQISIYREIYNEALGLYKSKKYLDAVGKFEEAMDIYVEAETYYSYG
                     KSLMNIGKFSESIKAYEISEALDYENKTNLYYNLACAYSLSNELESSIYNLKLSIQKG
                     FKHFSILEQDSNLVFVRKNNRWKHILMQIQTDKLLTKNVKDFLNNPSKKIKLISQSKT
                     INSINGEEIKTTEIELFEGIVFFKEDSIDKNNQHITLYQEGSYAIQKDKIQIELKKGK
                     KEISQYIDNTKKVLDLLQTPPLTFELLYKENLNGFMKNDFVIFGDDSLFVLDKKKCAY
                     IRDENSDCEGCKKQYEQKGYFCAY"
     gene            complement(24790..25413)
                     /locus_tag="PLF66_11910"
     CDS             complement(24790..25413)
                     /locus_tag="PLF66_11910"
                     /inference="COORDINATES: protein motif:HMM:NF014543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HMZ64795.1"
                     /translation="MNFKRSIILTLVLSLVAMTTLTNCSSGDKKEEPPVKKDDSKSAT
                     DSAKTSAPSTRAAGGDCVGDCKNGEGAMTFADGSKYQGQFKNEKFNGKGSLLFSNGDN
                     YIGDFKDGNMDGNGVYKYANGDQYEGQMKDGKFNGYGTLIGSKGTKYVGWFVDGKFNG
                     NGTFTTRQGRTYSGEFKDDLLNGKGTVKEKDGKLVYDGPFKDGQPVK"
     gene            complement(25633..26112)
                     /locus_tag="PLF66_11915"
     CDS             complement(25633..26112)
                     /locus_tag="PLF66_11915"
                     /inference="COORDINATES: protein motif:HMM:TIGR02246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgcJ/EcaC family oxidoreductase"
                     /protein_id="HMZ64796.1"
                     /translation="MTQNNINLNDKENIRSLYEKWIFAWNHQNAKSMSDLVSEDGDII
                     GFDGSHMRGPNQVYDTISQIFAAHPTGLYVSIVQEIKLLSETTAILRAVVGMIPRGGS
                     EINPAVNAIQSLIAVKKKEQWFIELFQNTPAAFHGRPEVAEQLTKDLRSALSEFNKN"
     gene            complement(26133..26582)
                     /locus_tag="PLF66_11920"
     CDS             complement(26133..26582)
                     /locus_tag="PLF66_11920"
                     /inference="COORDINATES: protein motif:HMM:NF017015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HMZ64797.1"
                     /translation="MILYELFPHWETRTNRILKSLENRTPYELDFKFRSNMRSLGNLY
                     RHIISAEIFWMEDVVAGRGQMFSEIDEKEFSNAKSISDKWRSVRNRSQKIVKPFSLSD
                     LKKTFQNPRKKSFDLNYILWHVVEHEIHHSGQISAMLRAQNLPSPIN"
     gene            complement(26579..27142)
                     /gene="pth"
                     /locus_tag="PLF66_11925"
     CDS             complement(26579..27142)
                     /gene="pth"
                     /locus_tag="PLF66_11925"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010573531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HMZ64798.1"
                     /translation="MKLIVGLGNPGDKYNNNRANIGFKILDVIANNINVEIKTKKKKS
                     MIGRGDFEGTEVVLLKPQTFSDLSGESVLYIASFLKIKVQDIVVIHEDTNLSLGQIVV
                     EKGGDDKSQLGVASIANSLRSKNFIRIRIGIKNNSFKGKDKDKFLKEDFSPAENLRLI
                     EIINDAEAAIRSISLGDIDEVVEKYRL"
     gene            complement(27154..27840)
                     /locus_tag="PLF66_11930"
     CDS             complement(27154..27840)
                     /locus_tag="PLF66_11930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016550507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HMZ64799.1"
                     /translation="MSRLLVKAVKRTTTGKNENNRLRAKGLVPINIIFDGKSLPGSVD
                     ESEINKVINSGIRSATLLDMDMEGDGKSSVFVKEIQRFPATNRVRHIDFYKVTPGKKI
                     TAKVAVKTSGVAKGSKSGGQFEHLIHELKIKSTPEDLKDLIVVDVTDLEVGQSIKVSQ
                     LNVPKSWEILINGDPIVTSVNVTKAILAAERNEKVAAAAAADPKAKAAPAKGGAKAPA
                     AAAAPAAKKK"
     gene            complement(27871..28809)
                     /locus_tag="PLF66_11935"
     CDS             complement(27871..28809)
                     /locus_tag="PLF66_11935"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016545874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HMZ64800.1"
                     /translation="MINDLAVFSGSSNRPLAESICSHLNMPVGKIILKKFSDGEISVK
                     IDENVRGRDIFIIQSISNPANDHLMELLLIIDALRRASARRITCVIPYYGYGRQDRKV
                     EPRVPISARVVADLLQTMSPHRILTMDLHADQIQGFFHIPVDHLYYSPVVVEYILSKK
                     MDDLVIVSPDSGGAERARFLGKRVNASLAIIDKRRPRANESEVMNVIGEIEKKHCIIL
                     DDMIDTAGTISKAANALLKKGAKSVMCCATHGVLSGSAADRLNEVDFSEVILSDTILL
                     PDAKKIRNLTKLSVGKLFANAIERIHNEESISSLFI"
     gene            complement(28806..29564)
                     /locus_tag="PLF66_11940"
     CDS             complement(28806..29564)
                     /locus_tag="PLF66_11940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010571098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HMZ64801.1"
                     /translation="MSQTDKICAVVLAAGKGTRMKSELPKVATLLNGKPLLLHVLDNL
                     IASGIKDIYVVVGFKKEVVMDLCKSYTNIHFVEQKEQLGTGHALLCCENSLKDFQGNL
                     LVACGDVPLLTSTSFKNLANFHLTNHHSVTVLSAEIDTPKGYGRLIRNNANQLLRIVE
                     EKDATDEERNVKEINTGTYIFNSPEVFQNLKKVNTENAQGEYYLPDLVKIANLKNEKT
                     GALVLNNHLESSGVNSPEDLQRLESYIAEGKIAV"
     gene            complement(29600..29672)
                     /locus_tag="PLF66_11945"
     tRNA            complement(29600..29672)
                     /locus_tag="PLF66_11945"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(29638..29640),aa:Gln,seq:ttg)
     gene            complement(29676..30569)
                     /gene="ispE"
                     /locus_tag="PLF66_11950"
     CDS             complement(29676..30569)
                     /gene="ispE"
                     /locus_tag="PLF66_11950"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010571099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HMZ64802.1"
                     /translation="MLSPAKINLGLKILYKRPDQYHELESVFLKLNWGDDIEFVENDS
                     GKIRLVSQNELSGIKYNLFEEVSERGDFTKNILFKTFEKVKTHLDSNLGIDVHLTKRI
                     PPGGGVGGGSSNAASLLTFLATKLSNEKRNLIMDFAPKIGADIPFFLRPGHFLVSGIG
                     EVLKPIQVASGFGILAIPPFSISTKESYLSLKKPLQTDWVQKSWNLLDEASTVALAQG
                     DWAKLQDRFENDFERYAFSEFSELEMLKQSLYKSGCSYVSMTGTGSCVYGFVQDRDHG
                     TDILKKMTQNYPKHYFIQFSF"
     gene            complement(30573..31541)
                     /locus_tag="PLF66_11955"
     CDS             complement(30573..31541)
                     /locus_tag="PLF66_11955"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HMZ64803.1"
                     /translation="MNPTKFRVSNKLNLIKIITENPGISLSKLRSYTHYKNNDQLKKA
                     LGELFMVGSYPYTPADYIEIDFNENETLNINLPVSLDKTIGLTINEWLAIRKILEEEL
                     SNPEVEDSYKSQYKSILDKIKRIIPYSEANAHENIKSKIEEAIQKKRKLSFYYAGWKN
                     ENPELRTVDPWFVFSEKNEYLVAYCNDRLGRRNFRLSSISNPQVLEQQINHYLDASEQ
                     NKHILEFNQFLNVSEQKSDMAHILFSKEIEFNLSKYAKLEIISNKINGNNQFIYAKTK
                     ILEKNWFLDMIKGFGNRIIILSPENLRAEVLSHIDQINIPKIIPSN"
     gene            complement(31590..32414)
                     /locus_tag="PLF66_11960"
     CDS             complement(31590..32414)
                     /locus_tag="PLF66_11960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64804.1"
                     /translation="MLPKISNDQLEAAFNKYKQERNIKLLLDTAGLWIMNKARYRYRL
                     DEDELSEVFIHFYENGQKCIEFFERNHYISFPAYLSVYTKHLAFSTYKRRKQIYNEEY
                     LGLWSEGMMIRDYIPHILDNTHRIRMHLKELCSMGRIIVSLRFNLKLEIEDLKILYKN
                     MTKPIEVFHQEYEDRIHRMRAKREDLNLTLNKYNHRIINSSNSSTHIYRARKQRIINS
                     LLDTYILYSIKEISDLLAISRHQTGRLYRRSMEVLRDRIYAQNEENDRKESFSIAA"
     gene            complement(32577..33041)
                     /locus_tag="PLF66_11965"
     CDS             complement(32577..33041)
                     /locus_tag="PLF66_11965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015676734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64805.1"
                     /translation="MKKIFFFIFAISLQLLSEPLKVGDEMTLLQFETQKEEKIIISKE
                     TRTLIFTSDMEASKIAHELFEKLGDDYLKQNNAIFISDIHKMPFLITKFVALPKMRKY
                     PYSIALIREEGPGFIFPREKGRLTVISLSEYKVSSIQFVENTEDLKNKIDRK"
     gene            complement(33096..33599)
                     /locus_tag="PLF66_11970"
     CDS             complement(33096..33599)
                     /locus_tag="PLF66_11970"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HMZ64806.1"
                     /translation="MNLTIEKASIENMDAIKNILSTTWKHTYSDFFYQETINKITHEW
                     HNVEVLSRQIQNEKVIFKVGSIQNQLIGIGTAFEVEENLIQLGRLYILPEFQRKGFGE
                     RIFKALLVDVRERKCKSFLLHVEEKNQKAFNFYKKIGFHPIRTIQEDVFGFRINLIEM
                     ELKIGDN"
     gene            complement(34127..36259)
                     /locus_tag="PLF66_11975"
     CDS             complement(34127..36259)
                     /locus_tag="PLF66_11975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010416921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HMZ64807.1"
                     /translation="MSANIKEKPTKESGERNFKKLSNVEHVRMRTGMWLGQNSMSTFE
                     QHFFKKDSKGNYEISHEELNDIPAKLKCLDEACMNAVDEYRKNQNDKALKDGEKMTKL
                     IVSLSADCGRVTVEDNGRGIPAKNAEGVFLHLMYGENFDDQVKQDHVAGQNGVGISLV
                     RMVSNFFRVTTENKGQSYKKLFTVHDEVRKLLRSFKFSPEEFEKIILYYDEHGNFKEC
                     PLLNKDHLSKLEPLMAKTFMIETIKPCGDSHGTAVEFELEPKYFNKLDTKFNPNLMRQ
                     YLQDIAMTNPGLEVQFHHKNKSEKFKFKKGMEEIFINSDLTYFKMDYKDPNSASQIHL
                     ETYFVIGQNKTLTWVNSNFAVQGGSAIEYLENRICDEVRKKSQIVSLEKKLKTQSTRN
                     DVRNCFHMYVNLRILNPRFKSQDKSYLINDLNEDIRNAVDKNLDKLIKKTDLLEEVKM
                     QMEKRTQLKELEDAQKGLRKASKNNIPKLMPPTGKPSDAGRVLFIAEGDSAIAGLRPA
                     RNPKLHGLFPLRGKPMNCKGMSLAKALANEELKNIVAILGLPINEKLKDAKELNYEKI
                     SIITDADFDGYAIRSLMLSFFYEYWPELFDFGVIHISSAPLFEVDVKWKDGKKETIFC
                     IDDKDYDKLMDRIRKNGGEMLRKKRNKGLGETGKEAMKFAVEECMTKIILSNRKSAQK
                     TQDLWFHKDFAEQRREAISEYSMAVIQD"
     gene            complement(36259..37641)
                     /locus_tag="PLF66_11980"
     CDS             complement(36259..37641)
                     /locus_tag="PLF66_11980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008595655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HMZ64808.1"
                     /translation="MKNTQETTRTDFPKVLFEDQVNDDQRKYSRYVCDSRAIPHEIDG
                     LKPVQRRILWAMWNSDARNRHTKTVKVAGLAMGYHPHGDKSIQDALSAMAQDFTFANN
                     IPLVHGEGTFGDVLDPNAIASPRYTEVKLSDFAKDLGFFEGLSDIDYVKNYDETEDEP
                     IHFVGKVPIVLLNQIQGIATGFRCFIPGHKLSDIIESQVAYLKTGKPKKIKPWYKDYN
                     GEVRVVTNENGSDVMYTTFAFKWEGESLFLTHAPQTWNRDKVIALLEEMIERKDNWLK
                     DYIDSSSQTFRIELVYKKGEKPTEKDIKALFNKENLDTLSFNVITHEGRLKNFKPEEI
                     IKRFCDFRKTHLIRRFKRLAGLEQEKIDRNSELIRFINEKWNQKVTNIKSKQEFENQL
                     KSAKFKYFEWLASIPVYRMTLEEVKKCQEAIAEAKTKFAEFTALSKQDNKLTAFMIDE
                     LTELRNKWDK"
BASE COUNT        12576 a         6683 c         6076 g        12407 t
ORIGIN      
        1 atgctgcggt tttgcatcaa aactattctc aatggcctgc gcccaagttc gttgttcgat
       61 gaactcaagc ttttccttga ttcgattgga atagattccg tagtagcgta tcatcttttt
      121 atttggatcg ggaagaaaaa ataacatctt cgccatgaac tcataaatag caatcgtttg
      181 atcctgaaag cttttctctc tagtatcttt ctctacccaa cttttgtatc gaaatgtaat
      241 ttcctttttc tcatggttta cagctcgaat ctgtgaattg tggaaaaaac cagaagcgat
      301 atattctgct gttctaaaga gaggttcttt ttcgttaccg tctatcggtt gaaagtaaac
      361 atgaaaacca tttttctatt tgttttggat aacaagagat tccgcttttg agataacttt
      421 ttctttttca taatgtttac agaatgcctt catccaacta aatgcaaatt tcttgtaacg
      481 cataaaagaa agattcaata cttccttggt ctctttgtgc ggttatataa aaagtcactt
      541 cgatacaatt ttcgtcagcg gataatgaca ggaaaacatt tcttttatta gtttttcttt
      601 atcgcacctg taacgaaaat cactcagtga ccgccttttt gcaacctcta acgagatgaa
      661 tatctggcgt atacaatcat gtccggttgg tgagtagcag aatccgagcg agcgtatcga
      721 accaccggaa tcgtgaaggt aacatgaata tgccgcaaat cgatatttag tatatgagaa
      781 agatttacag accaaccaaa aagtttcttt cgatagcaag ataaacatag tcttgactta
      841 caactaaatg gaacgattaa gtttacatca cattcttcac aagaatatct ttgaaatcca
      901 ttcagaaaat ctccacaacc taaaagtttt tctacctctt ctatcttccc ttccgaaagc
      961 tctccatact tcgatagaaa tttcatatcg tagattctta gaaactcttc ccaatgcttc
     1021 ttccaaatag atttgtattg actcgctctc tctagactgg gaatataatt tggctttttt
     1081 gctttgtctg tattgcacac atacattata gaaaacctct acaatagatg gataaaaaat
     1141 tcgtatgtat gtgcgggctt ttgggaaaaa aattctacac tgacgtggtg gagagtgttt
     1201 tacctatcgt atcaccaaac ttcccttaaa cttaggggta aacattggtt aaacttatcc
     1261 taaatatata tcactaccta tctttaagat aagtttagtc ctatctttag gggagcttta
     1321 ctgtaaaagg attccatcgt ttctatcgtt ctgttatcgg tggtaatctg ttaaaattgt
     1381 gggtaaggat gccaatataa aaagggactt actatacacc aaacgctttc tgtaattttt
     1441 aatatggaat ttttaggaat tgtgtttggt ggaaccgaac gagactacta gagaagaata
     1501 aactttcttt ctattcgcgt tcagtttagt agttcgtata gtcctgctat agtggttgct
     1561 atattaaaaa ttgattttaa atataccaaa gaataaagaa gctcaacatc aatccaatag
     1621 agaatgtgtt ttatccctta tcaagcattc ttgtagcaaa caatatccca atctgcaaac
     1681 ccaatcctcg atcggtcaat gctgtcaact taagaattac caccgaggac accgataacg
     1741 gaacgagaca gaaacgattg gcaattgaat tatcgaatct tttatcggtg tcctcggtgc
     1801 aatcggtggt aatcttttaa atttctatca catcgaactg gtgttattta gaatctaaat
     1861 cttttatggg acatattgtt cggacaaagt tctatttccc tttaaaataa tttaaaaaag
     1921 cctaatttta tgttgagatg cgccgattta gattaaaagg gtatctcctg tgaagacgaa
     1981 cttagaactt ttagaaaaga catttaaaga agaaattggt atctataaga atctgctaat
     2041 tttagagaca agtaagaaag aagcaatcct tcgttccaat ggaaagcatc tggaaaactt
     2101 tacaaagcaa acttctacca tcatggaaaa tcttgcaact gtggaagcaa agagaatgaa
     2161 actcgttgct gattttttct ccgatgaaaa aaatggagca gttgttccaa ctgtcacaga
     2221 atttctaaat cgtctagata aagttaccct agcaaaattt aaaccaatca caaatgaatt
     2281 aaaatttgta gtaaccgatt tgaaagaaaa actaacagtc aatgaagagt tactcaaatc
     2341 aaaactcgat atatttgctc tctctatcga tgcattaaag aatgcaggag aggctcaagt
     2401 atctgattta tacggcgata atagtaaatc gaaagttcgc acaaatgtca tgttaaacat
     2461 gagagcataa ggagtattta tgggatcaac attttcagga ttagaagtag gaaaaaaagg
     2521 attatctgtt caccaacagg cactccatac aacaggccat aatatttcca atgcggataa
     2581 taagcagtat tcacgccaaa gggtgcaaat tacatctgct gaaccactct atgatccagc
     2641 ttacaatcgt gcgttagtag ctggtcaaat cggtcaaggt tctaaagttc ctgaaatcga
     2701 aagaattcgt gatagcttca tcgacgatag aatccaagaa acaagttcta ttaaagacta
     2761 ctggtctgct aaaaatgatt atttatacag aattgaaatg attatgaatg aaccaggtgg
     2821 aatgagtctt cgttcccaaa tggatcaatt ctgggcggct tggcaagagc ttgcaaacta
     2881 tccagatgaa agcgcgcatc gatctgttgt aaaagaaaaa gctgtaggac tcggaagtag
     2941 aatcgaagac acataccgca aattaaccca attacaagaa caagcaaacc gagaagttga
     3001 atcaaaaaca aaccaattaa atgttttggg agaaggtatt cgtgcgttaa acgaaagaat
     3061 caccaaagca gaagctctcg gtgatagacc aaacgattta tatgatagaa gagatggaat
     3121 gctacaagaa ctatctgaac ttgtagacat cagcatagga agaagcgata aagatgaatt
     3181 catggttttt attggacaac aaatcttcgt gcaaggaatc aagaaagaat ctgttgaatt
     3241 agtaggcaat cctgaaaatg acggaaagtt caaactgatt tgggcaagag atggaaaaga
     3301 tgtattgctt cgctctggtc acttacaagg tctcatcgaa gttcgtgatt ttgtattaca
     3361 tgaaaaaatc gacaatgtag atacatttgc cattaacctc actgacacag tcaatgaaat
     3421 ccacaaagat ggatttggtc tcaatggaaa aacaaatatc aactttttcc aacctagaga
     3481 tttatctctc aactccaatg cagaatatga ttcagatgga gatggtatca acgataaaac
     3541 agcgatctac agagtaacag gtaaaatgac tctagatcca gcaagaccta tttctatttc
     3601 tggaaatatc actcttcaca aaaacgatga aaaatcaaca cccgtaatta ttccgtataa
     3661 cgtagatgat actttaaatg atgttatcaa aagaatcaat cgctctgaat ccggtgtagt
     3721 tgcttttatg aatcatgaca atcaacttgt aatcaaagca gtcactgcaa gtaattcacg
     3781 agaaaatttc atgattcgtc acttagaaga ttctggaaat ctactcgttg gtatgactgg
     3841 aattctaaat ggtagcgggg ttgctggatc ttttgattac aaccgattgg gtgaaatcaa
     3901 taaattccaa tccaatccaa gtgacatcac actcactcca caatttcatc ctgcttcttt
     3961 cgttcgtgtt tcggatgaga ttgcaaataa cgtcaatagc attgccgctg gacgtggtaa
     4021 agatgtgggt ggaacgggtg actacaacaa agcaaatggt caaaaagatg gaaataacgc
     4081 tcttttaatt gcaagcttct taaaagacaa accaatgatg gtaaattacg atcagaattt
     4141 tactgaattc tacaacggaa tgatttccaa aattggaaca gaagcaagag aagcaaaaca
     4201 agaactagga acgcaaactg cacttctcag ggaatttgaa aaattacgcc aatctgtaat
     4261 gggtgtcaac ttagatgaag aaatggcgaa catggttcag tttcaacatt cttacaatgc
     4321 agcagcaaag atgattaacg ttcaaaatga aatgttagat gttataatca atagactcgg
     4381 agtataaaaa tgagaattac aaatcaaatt tctaataata cattaataaa tacattagcg
     4441 agacatcaat ctcaattaga tgaaacacaa aaccaactcg gaactggttt aaaactaagc
     4501 cgtccttctg atgagccttc tgctgttgca aacaatatgt attttagaac tagagtcaac
     4561 gaattagacc agtttatgaa aaatgcagga gaaggaaaag cacgtctcca gctcatcgat
     4621 ggtcaattag aaagagttac agaaatttta caaagagtaa gagaactcac tgtccaagca
     4681 tccaacggaa tttaccaagg tgacaaaggc ttcgaattgg aagttgcgat tggaaaagaa
     4741 gtaaatgaac ttctaaaagc gacagttgat atagcaaata ctagagatgc tacaggacgt
     4801 tatctatttg gcggacatac aattgataga cctccttttg aatctgtaga agcaaagatg
     4861 aaaggcttga aaggaatcga agtacaagac caaatcatcg gagtagaata cagaggagat
     4921 ataggagatc aacttagaga aattgaacgt ggggaatatg ttggtgtgaa tgttgctgga
     4981 aacaaagctt tctggggaac aaacatgagc atcactgcaa atagagattc ttcctcctac
     5041 tctgcaactt ctgatcaaaa atttaaaatc gatggaacag aaatttctgt ttccgttggt
     5101 gacacgttag atgatatcat agataaaata aacagcgcat cgattgatgt aaaagcaagt
     5161 aaacttggta gcgacaatat tacattatca tcaactgctc ctcatcaaat ctggttagaa
     5221 gatgtggatt ctggaactgt tcttcgcgac atcggactta ttgaagtaaa ttatgcagag
     5281 ccgcctagca attattccaa agaagctact gtagttggaa attctatctt tgatgttatg
     5341 attcaactca gaaacgatct aactgcaaaa gaccaagaga gaatttctgg tagagatctt
     5401 gcggatatcg acatggcact cgaaaatatt ttgcgtcaca gatccatcgt aggtgctaag
     5461 atgaatcgtt tagaagagca tgaaaagaga attgattttg ataaaacatt tatggctgaa
     5521 ctcttagcta aaaatgaagg cattgatttc cctgaaacaa tcatgaatct caaatggctc
     5581 gaaacaattc accaatcagc tttaagtgtt ggctcaaaag tcattaaacc aaccctgatg
     5641 gattttttac gttaatatga ctaaagaaat tcaaacaaag cctttcggtg ctatgaaaat
     5701 agacgaaagg caaatactta gcttcccaga agggatttta ggctttgaaa aatacaatga
     5761 gtttgccctg attgaagaaa atgaagaaag tccatttaaa tggcttcaat ctttatcaga
     5821 actagatcta gcattcattg taatccaacc agatttgttt gcccctgatt ataaaccagt
     5881 aatcggacaa gaggatttag aaacaattca actctctcaa atagaagagg gagttgtaat
     5941 gacgatagtc accataccaa acgataatcc aagaatgatg actgcaaatc tgcaaggtcc
     6001 agtgattata aacccaaaaa acagaatggc gaaacagtgc atttccaaaa atgaaaatca
     6061 tccgcttcga aaattgattt tggaaaatgt tccaatggag agtgtctagt gttagttcta
     6121 gcaagacgtc tcaatcaatc catcattatc ggtaatgata tcgaagtagt tatcgtcgat
     6181 atcaaaggcg atcaagtaaa gattggaatc aaagctccta aaaatgtttc tgttcacaga
     6241 gcagaagttc accaagaaat ccaagcagaa aatatccaag ctgtaaatgt ttctatcaaa
     6301 ccagaagaac ttggcaaact cagtaatatt ttaaaaaaga aaaaataaat taatcctaca
     6361 tttttttttc agaataagcg cacaaaactt taaaaatttt tctctattat aataatactc
     6421 tatttgttcg tcactcaaag ctaattcctc aatttcagga gtaattcaaa aatctaatct
     6481 atacctaagg agtccaaatg caatctaagc aatacaatgg aaaaactatt tccaaaccta
     6541 aaatttgcta tcctaatgca aacctcaaac tttctgattt cagttccact cttctgattc
     6601 ctaataaata cctcactctt tttcaaacaa aactcatgca acacaaaggt gttgcaaggt
     6661 atatcgccta tcttttgtct aaataccaaa tctttattgc aaatggtatg atacctggtt
     6721 actcaaatgt aactaccaaa taccaagaga agggtcaaaa tctacaaaag gtctcatttc
     6781 gtcccagacc tgaagattgg gcagaactga aactctatcg agttagtttt ggtatgtcaa
     6841 tctctgcctt tttagtttac ttactgatag cagactcagt cgaattagca gcgaccgtct
     6901 cgaactactt agagactgtc ggaatttcgg cgatccctaa ttttcacttg agcgcaaaag
     6961 tatacctttg gaatcaaaag acatactatt caactgtttt tcaatatcga aaaagccatt
     7021 atcactaaaa agaccacaac taacgtttat aaatgactta ctaaaaaata aatcttagca
     7081 aaacactttt ctcaactccc aactcattta aaatttcttt ttgaatgttt gggtttttga
     7141 gaaggtgcag aaattgattt atttgttttt gatttatttt taaagaaaga ggctttgttt
     7201 tgtaaatgga ggaatctttt aaatagggaa gatgagtatc caattttaag ataggaactt
     7261 tcgttgtatc ggttagatgg taaagtgctt tttggttttt tgagcttata tctttttgat
     7321 aagagtgagg aatataggat tccatcggat aataacgatt tacctttgga acatgaaagg
     7381 attcgtaacc tgttcctcta gaatgggttt ttccattttc ttttttaaag ctaattcctg
     7441 agagggataa agatttcgct agaaattttt ctgctaatgc ttttgcgagt ccagcgacat
     7501 ttagagaggg attttgaatt atttcattag aactagtaaa tactacagaa tgtaaaactt
     7561 ggtccagtgg ataagaagta agacagagaa gaataggatt tttaaggcta aaaatataac
     7621 gattaccacc taaccaagat ataataggaa tatcttctgg aatatttcta aaatggtaga
     7681 tagttccacg accagagtcg atcgataaaa tataatcagg tcgaataccg ttctgcagaa
     7741 gtagataaca agcagtatcc gctgccaata aaaagaaatc gtcttggttt ttcttaatcc
     7801 attccagttg atcttccaaa ttgggagccg cgccaatata caatactggt ttattctgaa
     7861 taggaagact atctttaaat ataagaccag tattatctct atcagaacaa agctgtaagt
     7921 ttataaaata atttctgagc caagttcgtg caaaataaga aatagttgaa gcgttcaccc
     7981 ctagctttgc ggttatctta tcccgaaagg aactgtattc ttctgaatac attctagaat
     8041 aataaggaaa agcaaaaata aactttttca gttttaagta gtctttatag ggaaattcaa
     8101 ttagaggtaa aacagataat tccccaccga gttcttggaa ctttgaactt agatctagaa
     8161 tttttttttt acttctggaa tttggagaac ggaaggatct ggttcgactg aagttagatt
     8221 tatttttccg cgtgttagat ttaaaattgc ttcaatctga taacccaatc caaatccaaa
     8281 gcaaatcatt gtatcaggag agtaggatag ggaactgtat tcatttaaga ctgtttcagc
     8341 aaagcgattg ctttctttga taggatcatg cttagaataa ataaggctac cattctgaat
     8401 atttttcaga tttggtagcc catttgaatt tatatgaaga ttgtgagaaa tgtttagctc
     8461 ctagaatgaa ttagtcatcc tcatcatcta gatcatcatc atcgtcgtct agatcatcgt
     8521 catcctcatc gtctaaatca tcatcgtcat tgtcatcctc atcgtccgaa gcagaatcta
     8581 gttcatcaga atcgccttct gcaacaaagt catgtttagc ttcttcttct tgcgggatat
     8641 aatcctcttc aatgtcctct tccacaacag gtgctgctga attagaaaat agacctttaa
     8701 agcccttgta cggagcatca gacatatttg cctctgcttg taatttcttg cgctgttcag
     8761 ctgtgaaata gtcatatttc acaagaccat cagatagagc atagagggct tgcactgcaa
     8821 tttttctagt cttaagtttt tttggaactg gaactctatc aattttatcc aaagcagcga
     8881 atccagccac tgccaattca tattttttag ttcttgctag ttcaattagt ttgtgaattt
     8941 caaattcttg cgtgctcatt tatactccta taggaaattt aggtatttag tacaagattt
     9001 aggtataatt gcaaaaaaaa atcatagtac gaggcaatac gccatgattt caaggaaaaa
     9061 aaaccagtca atgattttaa taattaaaat ttattcttac ttgccttacc ccccctatac
     9121 ctagatatag ttaaaaaata catacaaatg aaacaattca tacctttttt gtttatacta
     9181 tttttacttt cctgttattc ccagaaacag tcgaactttg atgtatcctt atatgaaggg
     9241 cttgatttat ccggaaaaca aattgtctta aaagatctat cttataagag acttgctatc
     9301 aatgtctatg cacctacctg tgttccttgt gtaaaagaaa ttcccgctct aaatgcaatc
     9361 tataaagaca taagtaaaga taagtcccta ggactcttta tggttgtaga tccatatgac
     9421 attgtaccag atgcagaacc atccaatact tttgaacaat tatacccaaa agcaaaagaa
     9481 attatggaaa aagaagtaat cgaaaaaaaa atagagttac ccatattaat tatgaaacga
     9541 ccgttcaaag tctcccaagg ggatggactc ataacgggtc gaccagaaac tttattattt
     9601 aaaacgaatc ctttgatttt gtattacaat ttcattggac cattgagtga agaaaaagat
     9661 atttcaatca ttcaaaatca aaataagatc aaatttttta aattaatgtt aggaggagtt
     9721 taatgagaag cttaattgtg cgatttcttg attgtgaagg acctgggatt ctagaatcct
     9781 ctcttcgtaa tgccaattat aaaattacat atcatgatgc atataaaaaa ggaattcaac
     9841 ttgttcctga atcacaacag atttttgata ttattgtctt aatgggtgga cctcaagccg
     9901 tttatgatcc taagcttgaa tcttttttct acccttatct taagttagtc gaaaatgcgt
     9961 tatctattcc cgggaaaaaa gttttaggaa tctgtctagg ttcccaaatc attgcaaagg
    10021 cattaggtag caaagtagaa aagggtaaaa acggaccaga agttggtttt tcaaatgtat
    10081 ctgtatccaa ttcaagccat cctatttttt cagggatctc atctaagcag ttaacaacat
    10141 ttcacctaca tggggatact tttgatttac cgcaagggtc agaaagactt ttagcgagcg
    10201 agaaatatga aaatcaaatg ttttcctata aagacaaggc aatcggaatc caatgtcatt
    10261 ttgaagtaac ttatccaatg ttagaagtat ggtggaatat tcacaaagaa attccatcta
    10321 cacttggcaa gatatcacca gaaataaaaa cgaaacaaca agaaatggaa gcaaatgccc
    10381 gcatcctttt cgacaatatt ctaaaattaa aggcaaatta atcaaaagga aaaaataaat
    10441 cacaatgttt caaaaagtat tacaatttat tttcggaagt aagtatgaaa gagatctaaa
    10501 gcgtctaacg ccgatagtcg cccaaatcaa cgctttagaa cccgaaatcc aaaaactgtc
    10561 aaatgatgaa ttaaaaaacc aaacgcaaaa atttaaaaac ttattagctt ccggcaaaac
    10621 tttagatgat attttaccag aagcatttgc aaccattaga gaagtatctg ttcgcacaat
    10681 gggacttaga cactatgatg tccagatgat gggaggaatt gcactccatt ggggaaatat
    10741 cgcagagatg aagactggag agggaaaaac acttacctcc actcttgcag tttatcttaa
    10801 ttctttggct ggcaaaggag tgcatgttgt aacggttaac gactatttgg cacgcagaga
    10861 cgcgctctgg atgaaaccta tttatgactt cttaggagtt actgttggta ttatccagca
    10921 tgatatggaa cacgaagaaa gacaattggc ttatggttct gatattactt acggaacaaa
    10981 taatgaatac ggatttgatt acttacgcga taatatggtt cctagaaaag aacacaaagt
    11041 gcaaagagaa catttctttg caatcgtgga tgaggtagat tctatcttaa tagatgaagc
    11101 tagaactcca ttgattattt ctggagctgc tgatgaatct acagataatt acataaaaat
    11161 tgatacaatc attccaaagc tagttgaaaa agatgattat gaagtcgatg aaaaagcgcg
    11221 taacgttctc ttgacagaag caggggttaa gaaagtcgaa caaataatcg gtgtagataa
    11281 tctttactct cctgacaatg ttgaattagt tcatcatgtt caccaagcat taaaggcaca
    11341 taaaattttc cagagagatg tagattatgt agttcaaaac ggagaagttt taatcgtaga
    11401 tgaatttact ggaaggctaa tgccgggtag gcgctattcc gatggattac accaagcact
    11461 agaagcgaaa gaaaaagtaa ccattgctag agaatctcaa acacttgctt ctatcacttt
    11521 tcaaaactat tttagaatct atgaaaagct ttccggtatg acaggaacag cagataccga
    11581 agcagaagag tttgcaaaga tttacaaatt agatgtgatt gtaattcctc caaatatcaa
    11641 gatgaaaaga atcgaccatc ccgatagagt ttacagaaca gaaagagaaa agttcaatgc
    11701 aatcatcaat gaaatcaaag aacttcatgc aaaaaaacaa cctgttcttg ttggaaccat
    11761 ttcgattgaa aaatcagaac ttctctctaa tcgactcaga atggcaggtc taccacataa
    11821 cgtcttaaat gcaaagtttc atgaaaaaga agctgagatc attgcgaatg caggcaagcc
    11881 tggagcgatc acgattgcga ctaacatggc aggtcgtgga acggatattg ttttaggtgg
    11941 tgctcaaaaa tttaaagact taatggaaaa atggacagaa gttgatccac aaattgatga
    12001 atttaaatta gcaatcaaga aaaaagatta cgatacagct aaacaaattg cagaatcttt
    12061 ttcacaacac ggtaagaaaa agaaagcaaa ggaaattata gacgaagtat taatctggga
    12121 aaaagctcat gaagaagttc taaataatgg tggcttacac atattaggca cagaacgtca
    12181 tgaagctcgt cgaattgaca atcagcttag aggtcgttca ggaagacagg gagatccggg
    12241 ttctagtcgc ttttatttat ctctagaaga tgatttaatg cgtatctttg gatcggatag
    12301 aatttcttcc attatgcaaa gacttggaat ggaggaaggg caagagattg aacataaaat
    12361 ggttagcaat gcgattgctc gatctcaaaa gcgagtggaa gggcacaact tcgatattcg
    12421 taaacattta ttagaatacg atgatgttat gaatgcacaa aggattttca tctataatct
    12481 tcgaaatgac gttctaaacg aaaaagcaaa tatggaggaa ctcatacaag actgggtcaa
    12541 agaagttgta gacctccaaa tagaagcaca ttgccaggga aaaaatccag ccgcatggga
    12601 ttatatttct atgaatgagt ggctcactac tatgaatgta tccgtccgaa ttcaagatgg
    12661 agaattttct agatcgtctg aagttcaaga aagacttcgc gagaaactag ttactgagtt
    12721 tgtaaatgta tacaaaggaa aaatatctaa ggtcggtaaa gaggtttggc agtatttaga
    12781 aagaagcata ctactagaca tattagatca tagatggaaa gagcatttat atgtaatgga
    12841 tcatttacga gatggaatct ggaccgttgg ttatggtgag aaaaatcctt tagtcgaata
    12901 taaaattcaa ggttttaaaa tttttgctac aatggttgac aatctaaaag cagaagtaat
    12961 tggctttttg atgaaagtag aagtagcgga agacactaag attgaagaaa aagaaattga
    13021 atatagaaaa gtaggcgaag aaagcactga catagattta ttcaatatga atggaaatcg
    13081 aaatgatctc attcaaacta ccaataaacg taaaatagac acaaatgtaa cctcaggaag
    13141 ttctgggggc tcgtcatcta gaaagagcag tagacgaaag cgttagatgc tattaaatga
    13201 gattctaatc aatacaaaga caggagtatc ctcctgtcct tattgcaaag gtgtgggaat
    13261 tgttttagat gaggttatac ctggttctcg cactggttca ttaagtctat gctcctgcgt
    13321 tacagaccac tgtatcccct gccctgctaa agctaaagca ccttatcgga tctatgaaga
    13381 gtccatggat aaaatgattc cttgtttttg tcaggaagca cgactcacaa ggaatagact
    13441 ccattacttg atcgaatcat caaacattcc tccgagatac agatatcgat ttttaaattc
    13501 tatggatgtg tctgggaata atatgatctc tcttttcgtt gcacatgatt gggcaaatga
    13561 attgatcctg aattggaata atccaactca ttggaaagac aaaccaaagc aaggaatgta
    13621 tttatcagga gggacaggtt ccggcaaaac tttattagcc tgtatcatat taaatgagct
    13681 catttttcgt tatggagtaa aatgccgcta tgcgaaaatt agtaaagatt ttctaaacgc
    13741 actcaaagac acttaccaaa aagacagtga ataccatggt caagaaagaa ctatagaaat
    13801 ggaatttgca gaaatagatg ttctagtaat cgatgacttt ggagttcaaa aagactctga
    13861 ctgggtgaat gcaaaattat acgatttaat tgattctaga tatgaaaaag aaaaaataac
    13921 tctactcact tcaaatttac ctctttatga ttggaaggaa aaaggagaag gtagagtata
    13981 ctcaagactc tgtgaaatga ctcatgaaat taagttagaa tgccccgact atcgtctcaa
    14041 attccaaatg agcacttagt ctactttgtc aaacaatcta aaacatactg cttttatatc
    14101 acttgggtct aatttaggag atcgaaatct ttttttacga aaagcaattc aggcaataga
    14161 agagattcct tgtaaaataa ttcagcactc aaaaataata gagactaaag ctttagattt
    14221 tatagatcag ccagactttt tgaattgtat tcttgtaatt aaaatagatc tgtcgcctat
    14281 cgaattatta gatgccttac aaagtattga aaacaaatta ggaagaataa aacgttttga
    14341 caagggacca agagaaattg acttagatat tcttacctat gataatctaa tttttaaaaa
    14401 tgaaagactt gttttaccac atcattccat caaaacaaga ccatttatta aagaactatt
    14461 agaggactta aaataaattg atttagaatt ttgctctaac taaattcaat tcgatctgat
    14521 tcatccaaat caacggattg aagaattata gaaaaaaatc tcgcttgatt tattgctttt
    14581 taaagtcttg ggacttcaaa gtgcagctca tcaattatat acaacaaaat acaaaaattc
    14641 tattcagact ttttagttat aaatcttttt cagaagaaaa tactctaggt agtgatattt
    14701 ttaaaattct attctataaa aaaataaaaa gtggaaccaa ctcctatctc actttctgcc
    14761 caaactttcc cattgtgttt ttcaatgata tgatttacta tggtgagtcc catgccgatt
    14821 ccttcactag gttctagttt ctgaaatggt ctaaatataa ttctctcact ctcagaatga
    14881 aagccgacac cattgtcttt cacaaagaat atattttttc cctctttctg aatagaaccg
    14941 acttctattt tcacgatagg tttatttttt gaaaatttaa ttgcattatc taataaatta
    15001 taaagcacaa tacgtattag ttttctattt ccaaataatt gaagattgcc ttgaatgcta
    15061 aattctaaat tttgttccga atagagtttt tctatttcgt tcattaaatt tctgcaaata
    15121 gatgtaatat caatcgtttc catatctaaa tttgcatcag tgatatttgt cagtattaat
    15181 aaactttcta ttaaatagga tgcgttagca gtttgctttt gaatattctc taaatgttta
    15241 aattcatttg aatctatttt atctactaat ttttccttca aatactctgt tgaatcatga
    15301 atagatttaa ggggagcatt gtcgtcactc gctataatag attgaagcga atccaattct
    15361 aaatgcatca gatctaattc ttttgttttt tgagaaaagg atctttgcaa ttcgctattt
    15421 aatttataaa cattttcttc tgactttttt ctctctgtaa tgtcaataac gactagttgg
    15481 attgcaacct tgcccatata atcaaatcga ataccggaaa cttctacatc aatcactacc
    15541 ccatctaagc gaataaattt ctcttctatc ggaagtaaat gtttatctcc agttaaaata
    15601 gaaccaattc tatcaacaac aagatctcta taatcaggat gaacaaagcg cataacgctt
    15661 tgaccaatga tatcttctgg atttttgcct cctaaaagtt caatgcctgc agtatttgca
    15721 aatacacaga ctccatctag atgaacatat attccaaaag gtgataattc aacgagactt
    15781 ctataacgtt cttcttctac gcgtagtctc tccatcatat ttcttctttc tgtcacatca
    15841 cgaagagaaa ttaaaaagac cggttcattt tcccatatgg tttcaactac tctcatctct
    15901 gcaatagaat ctggttgatt tttagcatgt ataacaatct cggcaacttc tccctgtaaa
    15961 atcgggtagc cgaatggcat attgattagc ctttctttca tcttatgaaa taaatactct
    16021 gctgcagggt tagcaaaacg tatcatccca tcatgaccaa ctattaagat aggaattgaa
    16081 ttatgtgtaa ttattttttg aaatcttatt tcgctagaaa caagctcttt tctaagtgct
    16141 tttatgattt cttctttgtg gttttcagat tgtttatcca tacacttttt ctaactcgaa
    16201 agattctaac aactttcgtc tattattaaa atttcctaca acacgtatgg acttttctga
    16261 attttcttta atgagtgtag gtgtgactaa aatctttgca gcttcagcta ggtaaggatc
    16321 ttctgatata tcaaagataa ataagtcaca atcggatccg acttcttctg caatgaaact
    16381 tttcactgtc tgaattgcaa tttctgaaga ctgagctttt cctgacacat agaggtaaaa
    16441 agcgagtttt tccatgttct atattacatg aattttcatt ccgtgcattc agcaagattt
    16501 ttttaagttc aggatttaag gtttaaactc aaagtctttc attctttgtt ctgatatacc
    16561 aatcaaatca tgtttaattg taaaactatc gcctctaaac tctttatcaa tattaagtct
    16621 cacaactcta taccagaact ctttcggttt tacagtaaat gcatgattaa tacataaaca
    16681 tttaaattta aatccaagag aatattgtgg aggatccaca ctgtgaggag ctgcaaaaaa
    16741 atgaattggt ttatctgtct gattttgcat tcccaaaata aactctttgt atctaccgga
    16801 ttgaattaca atggtagaat ttggaatttc tatcgaagcg ggagaggaat ccttagatgg
    16861 aaccgacctt gtctcccaca actcatagga ttgttgtgat ttgatttcat agattttcat
    16921 ttcaagaggc aaatcaatat atgcccatga aatttgagct tttataaatt cagatttttt
    16981 ttcttctgtc gttaaattag gattcgaatt cccaatgttt ccattctttt ccttacatcc
    17041 aactaggaga aaaacagcaa aaccaattat gcataaaaac ctaaacataa aaggctattt
    17101 ggtagttact acttctggat cactaatcga ttcaatggat tctacatggt ttacatttgc
    17161 cataggttta ggcacaggat tcaataaatt taaaagacca tcacttgcaa tttgtaagat
    17221 ttggatcttt tcctttaatt ggcttgtgtc aaagttattt tctgaaagcg agtagtattc
    17281 gctcatcaaa tcgttataag cactttgcac atgaaaaata gacttttcca tagatacggg
    17341 agatgctatt tttcttttcc tataaacacc tgtgaaatat ttgtatatta aagttaaaaa
    17401 ataaaaagaa taaacaggaa tcaaaagaaa taaagcggat ataaaagttc tatccattgt
    17461 atcactaaat ctcttcttat ttaaaacgcc taccttcgcg atgataaagt agactccaac
    17521 agaagcgagg acagcaaaga gaaaattgat tttagaagta gatggggaca cactattaat
    17581 aagaatgagt atgataagag aaaaaaataa cagaacaaaa acttctagag ggataatttg
    17641 aatcacccgc tctataaaat caaacacacg ggagtagttt tttaataacg atttaaaata
    17701 cttttcaaag ttgttataat cattttggag aatagataaa aaatcttttg ctggcgacat
    17761 tatgaaaacc cctgtttttc ttattaatat attcggtaga gtctcgaaaa agtataattc
    17821 attaagtttt ctaacgaaaa ttatttccat ttatactcta ttagctatcc cattcctata
    17881 cgcacatgag agtcatttac catggttcgt gattctaatc ctaattttaa caaccctttt
    17941 tgtgtctttg tcgactctct tcttgagtat tctgatccta aaaaagttat ttcagaagga
    18001 ctttatcctt tggcaagaag cactctatac tgtatctgtt atactcatag tctattcttt
    18061 tggcttcttt gaaaaccaag ctggagtcat tttcttgctt tttgttttac tcttctttct
    18121 attttataga gcagtcagag tggttgttta ttatcgaatt attttagtcg gaatggctat
    18181 ttttgtaaat ggacttctaa cttttaaata tttacagttt tcagaaatat atttattcaa
    18241 tcgttccatt caaacaaaat acaatgaaca tgaaatggat ctatctcaat ggtcattctc
    18301 agaagaaact cgtatactta aaaatgaaac actaagattg actttaaaac ttcccaaaga
    18361 ttttttcttc cataatccaa aagacttaaa gatgaaagaa aaaactggaa caggacaaat
    18421 agctggtatt ctctcttcct cacaagtcga tccaaaccgt tatccgctcg ttaggatctt
    18481 ttttgtccca gagtttataa aggtagattc aactacaatc agagaggaat attcctccat
    18541 tttagatttt gagaaacacc aaggaaatat ggaagaaatt aaaaatctag gtgagagact
    18601 tcatcctgaa agaaaatggc agggtaactt ttggactttt tatgatatta tacgacctag
    18661 atattcaaaa actggttttt acatttaccc tttgaaagaa ggaagctatt taattttaga
    18721 tatcacagaa aacttagtag ataaacaatt tcacgaaaat gaaattgaag aaattattac
    18781 aagtattcag caaaactaaa attgatattc aagcttaacg gataacgtat cagtaaatgc
    18841 agaagctcgt agagataaat tatctttagg tctaaaaatc actacatcaa ataaatttaa
    18901 taaataaaca cctgctaaaa ttcctgccga aatttctaaa cgatgtaagc tcctatctaa
    18961 aactgctttt tgagcagtca tatctgctct tgctaaattt tgagtaaacg aatctctata
    19021 atataaagca aacatgtaat tctttgcttt ataattatag tcatcactct ctgatttata
    19081 ttctttatac tgataacctg taatccctaa tccaccaaga aaaagaataa aataggcagt
    19141 tgcagcgatt tgtctatttt gatacatctg tccccagcca ggcaatacca gtgatcgaag
    19201 aaacgccttt cctagagtga tacttcttct tcctccaact ggatctaata cgggagttcc
    19261 aggagaaaca tcaaccattt ctgctccacc attttccttc agaatctgat tgatttcttg
    19321 ttgagttaag tctttatagg aaacttttag aacttctttt ttggagaaaa cttctgggtc
    19381 tttagcatct ttctcctcaa aaacaatcga aagtttatcc tgacttacaa ttcgaccttt
    19441 ctttacattt ccatttcgca ttataattgt ttctgaaaaa actgcacttg agagaagaag
    19501 aataaataga tataagaaga tttttaattt catgacgcaa ccaaagattt agtttagtcg
    19561 attattattt ggattgaatt ggatttgtca atgaattatt agaagttctt ttggtggaac
    19621 aaatgcaatc tgccatagtt caaacttttg ttcgaaccat gacagtaaag tatatactat
    19681 ttttggatgt attcttttat gaaatgccta gtttgttcta ggatttcagc aaattatgat
    19741 taaacctctg tgctaatgct tccagattgt ttaatagcaa accatgctac tccagcccca
    19801 acagctcctg cgaggataac aaccattata ccctcaccag atgcgatgga aacatgttga
    19861 ccagcgtgtc ttccgtatcc cataaaaaac aacattggaa cggagaggaa tgtattgatt
    19921 ctagaaaata atagagcttt cttaggaata gatggatcag ccgggttacc acttttcact
    19981 ccattaataa tttttctttg atttggccaa ataacaaacc atacattgaa ccacataata
    20041 gaacctaaga gcattccaag tagaatccag actgaccaat ttggagttgc agctcttact
    20101 ccacccattt caaaaaagag aacgtaatag gttaatcctg ataaaaatgt aaacattgcc
    20161 gcccatctaa accaaaagag aacacgtggc attaattcag gaacaagaac tttttttact
    20221 cctgcatcta attttccttg taaaggaacg ttgactaaat ttaaaaaata gagaagtcca
    20281 atccaacaaa cgccggaaac gtagtgaatc cagcgaaccg cataaagtaa taaaccatta
    20341 ttatcttcca tttcttactc caattatata tctattattt ggactaattc agataaacag
    20401 tatagaatcc agattagtct ctaatttaac taaattagaa tgattctaga tttgacaagt
    20461 tttttaaaga atgctttcaa ttcttttcag aatcttttcg ctatttggtt ccacagatga
    20521 ctcaaatcga tctactattt ttccattttt atcaattaag aacttttcga agttccaacc
    20581 aatctcccct ttcttgtcag aattttcagt taagtattta tatagaatat gtttatcttc
    20641 tccttttaca gaaatttttc ccatcatatc aaagcttaca tcaaacttta acttacagaa
    20701 ctttgcgatc tcatcattac ttccaggttc ttgcgcaccg aaattatttg caggaaatcc
    20761 gattatttta aaacctcgat ccttatattt tttatagatt ttttctaagc cttcatattg
    20821 ataggtgtaa ccacattttg atgcgacatt cacaatcaaa actggagatc ctttgtagct
    20881 ggaaagattc acctcatttc ctttgatatc ttttactttg aaatcataaa tgctaaccgc
    20941 ataaatattt ccaataaaac ctaagaataa aatgattaat atttttttca tacacttttc
    21001 ctctcttcat tagtctgatt ccatttgttc caattttata ttttttagga ttacaaatgt
    21061 taaatgataa attctaaaaa ttaaatttct aatatcagga ataactttgg gcaaacaaaa
    21121 aattttaaac ttcaaagaag tgaaagttta ggaatactaa attcaatttg aaaaatattt
    21181 caaataatga aaagaatgta tacgattctc cttttaaaaa attctaaaga gataaaaatc
    21241 gaatcacaaa agaaaaggaa agaattaact tttattgact ctatcaaaaa ggaaataact
    21301 ttagatatat gaaacatttt agtatgtttt tatttgcatt cattatattt acccaatgta
    21361 tgccttcacc agatttagca gtctatcaat ccttaaaaga tcctaagatt tcaactaagt
    21421 cttctataaa agtattaaca attacactca ccggtgatcc aagtcaaacc tcaggagaag
    21481 cattcggaaa attatataaa ttaaaatata aattaaaaaa tgattctcta aaagaggctt
    21541 ggcctagagc aagatggcct aaaccttttg aaacaccgcg caatgaatat gttggaatct
    21601 ttgcattacc agtatctaac gaagtcgatt ccattcccga gaatattgaa aatcctgaac
    21661 ccaaagttca gttagaaacc tggaattacg gaagcgttgc cgaaatttta catataggat
    21721 cttatcaaaa tgaaaatcct acagttgaaa aattaaaaaa atttatttca gaaaatgact
    21781 atgaaattat tggattccat gaagaagaat atttaaaagg acctggtatg tttttcaaag
    21841 gaaatccgga cgaatatcaa acaatcatac gttatccggt aaagaaatta gaaaaaaaat
    21901 agatttggtt ttatataaat tgttttcgta tccctttata cttagccttt tcttatttct
    21961 tcttgtcttt aatgcctctc tattttctca agcaacggaa acaaaggaaa agccagctaa
    22021 gaaagattta ttcacagaag aagatgaaaa ctctccagca gtagaaagat ttagaaaaag
    22081 ggaatttgaa ctaggtgttc gtttcggatt aggatatcat gcagaagata gatttgaatc
    22141 ccaactcaga aattataaat cggagtcttc tccctatata ctcacttcag tttatttgtc
    22201 tccaattcga tcaacaacaa acaccgagat tttggcacgt attcgcttct ttgataatgg
    22261 aaaagctggc tttgtgtatg gaaatactca attcaagcca ttccatttat ccgaagttac
    22321 aagtctgaat tttacaaact tttacacagc aagtagacta aacttcaatc taaaagtaga
    22381 gtatttcttt ctaacctata cacatgaatt taaatttaga aatttttatt tagaaggtgg
    22441 aatgggttta ggtatcaata ctattagttg gaaaacaaat ggtgatacaa tctcgcgttt
    22501 tgaatacact ccacaatcag gctttatggt tggaaatgga ctaggatata aattagaagg
    22561 ttcatttaat aagaaggtta ctgataattg gattctacaa ataggttcct tttataatta
    22621 ctatactgtt ccttctttta gcggacaatt caatgatgaa accagttcct tttacttacg
    22681 atcagatgga agtatagctc ctctttctac agctcagttc agagactcta ttgtagatac
    22741 caattatgca tctagacaat tggatatgag ggctggaaat ttagttcttt ttctatcatt
    22801 gatttttcgt ttctctttat aatcttcctt caaatcattt cccccacgag cattccttaa
    22861 aatacattga gggaatacac gttatcaagg ctctcttaaa ttgcttggac agagagagtt
    22921 tctacctctc tcaatctatc acttcttatc gagaataatt aaagttacaa ttaactgatc
    22981 cgatttaatt tatttttttg taagatggag tctatcggca gtaattcaga ttctttatct
    23041 caaaatataa attatgaaag aaaatttgca tacttaaatt taggtatagg tgaacaggga
    23101 tagagaaaag agagataaag acaaaaccct atctatgact aaccgaagat aattttccat
    23161 tgaaagaatc ctgaaaattc tttttcaaaa agaatagaaa cctaaactct ttgattcaag
    23221 taaaataaat tccataaacc atccagttta gtaataaatt tattccagtt caatgtaatt
    23281 tgaagttgtt ttttcactgc aaatatatcc taatttaaat tgtaataaat aggttctaag
    23341 aaaatttagt aaacatctct tgttatattg tatatcaaat gataacaata agaacagata
    23401 aaaacggata aggattctat aaatattgat tcttaaacgg tttagaaaca agtcagaata
    23461 taaagtatga gtcgacaaaa gttgaactaa gcaattacaa ttcaagcatt gaaaagaata
    23521 gtagtagaga cgttaggatc atctaaaatc gaacaaatga agtgtcagct gttcatctta
    23581 agatgattta agttagtaaa gagaatttta tattttgaat ttgtaaaaat gatcacgaaa
    23641 tgggtaaata cttttggcat ttagattatt aaaaaaaacg aagaattttg ctgctaaaat
    23701 gatttattgg ttatcaatca ttctttcttt aattttccat ttcaattgca gtggtgaagg
    23761 aaaatcagaa aaaataaaac ctaattatct aacactctca tatgcaaatt ctaaatctgg
    23821 ttatgaaact gcaaatctca gtatagccga tcataataaa cttcaaaacc aaattagtat
    23881 ctacagagaa atttacaatg aggctctcgg tctatacaag agtaaaaaat acttagatgc
    23941 tgtcggcaaa ttcgaagagg caatggatat atatgtagaa gccgaaacat attacagcta
    24001 tggtaaaagc cttatgaata ttggaaaatt ttcggaatca attaaagcct atgaaatttc
    24061 tgaagctcta gattatgaaa acaaaacaaa tctttattat aacctcgctt gtgcctatag
    24121 tctttccaat gaattagagt cttccattta taatttaaaa ttgtccattc agaaaggatt
    24181 taaacatttt agtatattag aacaggattc aaatttagtt tttgtccgaa aaaacaatag
    24241 atggaagcac atactaatgc aaatccaaac agataaactt ttaacaaaaa atgttaagga
    24301 ttttcttaat aacccaagta aaaaaataaa attaataagt cagtccaaaa caataaatag
    24361 tataaatgga gaagagatta aaacaacgga gatagaacta ttcgaaggaa ttgttttttt
    24421 caaagaggat tctattgata aaaataatca acatattaca ctctatcaag aaggttctta
    24481 tgcaattcaa aaggataaaa ttcagattga actaaaaaaa ggcaaaaaag aaattagcca
    24541 atatattgat aatacaaaaa aagttttaga tttattgcag acgccgccac taacgtttga
    24601 attgctttat aaggaaaatt taaatggctt tatgaaaaat gactttgtta tttttggaga
    24661 tgattcttta tttgttttgg ataaaaagaa atgtgcttat attagagatg aaaattctga
    24721 ttgtgaaggt tgtaaaaagc agtatgagca gaaaggatac ttctgcgcat actagcgatt
    24781 aaccgttact tattttacag gttgtccatc tttgaaagga ccgtcataaa caagctttcc
    24841 atctttttct ttaacagttc cttttccatt taataaatca tctttgaatt cgccggaata
    24901 agttctacct tgtctagttg taaaagttcc atttccatta aattttccat ctacaaacca
    24961 accaacgtat ttagttcctt ttgaaccgat taacgttcca tatccattga acttaccatc
    25021 tttcatctga ccttcgtatt ggtctccatt tgcatacttg taaacaccat tgccatccat
    25081 attaccatct ttgaaatcgc cgatatagtt gtcaccattg ctaaaaagaa ggcttccctt
    25141 tccattaaat ttctcatttt taaattgtcc ctggtatttg cttccatcag caaatgtcat
    25201 tgccccttcc ccattcttgc aatcaccaac acagtctcca cctgcagcac gagtgctagg
    25261 agcagaagtc tttgctgaat cggttgcaga tttagaatca tcttttttaa caggtggttc
    25321 ttctttttta tcaccggatg agcagtttgt aagggtagtc attgccacta gtgacaatac
    25381 taaagttagt attatgcttc ttttaaaatt catatcaaaa aatctccttt ttatacaaat
    25441 acggacaatt actcgtatta gcatacagta attttattct aaaatcgtat ttggtctatc
    25501 atttttctcg gttctatatt tgtcacactt ttttacaaaa agacagaata gttcaggaaa
    25561 ggaaaattaa atgattcaaa tttgaaggat caaattttaa atattgtttc aatttaaaaa
    25621 aatggatgcc tgctaatttt tattaaactc agataatgca gaacgtaaat cctttgttag
    25681 ttgctctgca acctctggcc ttccatgaaa tgcagctggt gtattttgaa aaagttcaat
    25741 aaaccattgc tcctttttct taaccgcaat caaggattga atggcattta ctgcaggatt
    25801 tatctcggaa ccaccacgcg gaatcattcc aacaactgca cgtaatattg ccgttgtttc
    25861 ggaaagtaat tttatctctt gcacaatgga aacataaaga cctgtaggat gtgctgcaaa
    25921 aatttggcta attgtatcat agacctgatt cggacctcgc atatgagagc catcaaatcc
    25981 tataatatct ccatcctcac tcactaagtc tgacatactt tttgcattct gatgattcca
    26041 tgcaaaaatc catttctcat aaagagaacg tatgttttct ttatcattta gatttatatt
    26101 gttttgggtc attaaataag cgttatgcgg gattaattga taggactcgg taaattctga
    26161 gcccttagca tagcagagat ttgacctgaa tgatgtattt catgttcaac tacatgccaa
    26221 agaatatagt ttagatcaaa agattttttt cttgggtttt gaaatgtctt ttttaaatca
    26281 gaaagactaa aaggcttaac gatcttctga gaacggttgc gcacagatct ccatttgtcc
    26341 gaaatagact tcgcattgga aaattctttt tcatcaattt cggaaaacat ttgaccacgc
    26401 cctgcaacta catcttccat ccagaaaatt tctgcagata taatatgtcg gtataagtta
    26461 ccaagagaac gcatattaga tctaaattta aaatccaatt cataaggagt tctattttct
    26521 aacgatttta aaatacgatt tgttctagtt tcccaatgag gaaataattc atataatatc
    26581 ataagcggta tttctccaca acttcatcaa tgtcgccaag gcttatagaa cgaatagccg
    26641 cctctgcgtc attaatgatt tcaattaatc gtaagttttc tgctggtgaa aaatcttctt
    26701 ttaaaaattt atctttatct tttcctttaa aggagttatt cttaattccg attcggattc
    26761 ggataaagtt tttagatctc aatgagttag cgatagacgc aacgcctaac tgtgatttat
    26821 catccccacc tttttcaaca acaatttgac caaggctaag atttgtatcc tcatggataa
    26881 caacaatatc ttgcaccttt atttttagaa aagatgcaat atacaaaact gattcaccag
    26941 atagatcgct aaaggtctga ggcttgagta gaacaacttc agtcccttcg aaatctcccc
    27001 gcccaatcat agattttttt ttcttggtct tgatctcaac gttgatgtta ttcgctataa
    27061 catctaaaat tttaaaacca atattcgccc ggttattatt gtatttatcc cccgggttac
    27121 caagccctac gattaatttc attttttatt ttgttacttt ttcttagcag caggagcagc
    27181 agcggcagca ggtgcttttg caccaccttt agcaggggca gcttttgctt ttggatctgc
    27241 agcagcagcg gcagcaactt tttcatttct ctcagcagca aggatcgctt tagttacgtt
    27301 cacagaagta acaattggat ctccattaat caaaatttcc caactttttg gaacgttaag
    27361 ctggctaact ttaatacttt gtccaacttc taagtcagta acatctacaa cgattaaatc
    27421 ttttaaatct tctggcgttg atttaatttt tagctcgtgt atgaggtgct caaactggcc
    27481 accactcttt gaaccctttg caactccaga tgtcttaaca gcaactttag ctgtgatttt
    27541 cttaccagga gtaactttat agaaatcaat gtgtctaact cgattcgtag ctgggaaacg
    27601 ttgaatttct ttcacaaaca cagaagactt tccatcacct tccatatcca tatccaaaag
    27661 tgttgcactt ctaattccag aattgatcac tttattgatt tctgattcat ctacactacc
    27721 aggaagtgac tttccatcaa agattatatt gattggaacc aaaccctttg cacgaagtct
    27781 gttattttca ttctttcctg ttgttgttct cttaaccgct tttactaata atctactcat
    27841 aatttttgtg tcctttaata ttattacatt ttatataaat aacgaactaa ttgattcttc
    27901 attgtgaatt ctttcgattg cattcgcgaa taattttcca actgatagtt ttgttaggtt
    27961 tctaattttt tttgcatctg gcaataaaat cgtatcagaa agaattactt cagaaaaatc
    28021 aacttcattt agtcgatctg ccgcactacc agataaaact ccatgggtag cacaacacat
    28081 aacagatttt gctcccttct ttaagagcgc attcgctgct ttggatattg tccctgctgt
    28141 atcaatcata tcatccaata taatacaatg tttcttttcg atctcaccaa taacgttcat
    28201 tacttcagat tcatttgctc ttggacgacg tttatcaatg atagccaaac ttgcattgac
    28261 tctctttcct aaaaatctag ccctctctgc cccacccgaa tctggagaaa caatcaccaa
    28321 gtcatccatt ttcttactaa gtatatactc cacaacaact ggagagtaat aaagatggtc
    28381 tactggaata tggaagaatc cttgaatttg atctgcatgt aaatccatag ttaaaattct
    28441 atgaggactc atagtttgta ataaatcagc aaccactcga gcagaaatcg gaaccctagg
    28501 ttcaacctta cgatcctgcc gaccatatcc ataatagggg ataacacatg ttatccttct
    28561 tgcagatgct cgcctaaggg catcaatgat taataataat tccattagat gatcgttagc
    28621 cggattacta atagattgaa ttataaatat gtctctacct ctaacgttct catctatctt
    28681 tactgaaatt tcaccgtcgg aaaatttttt taaaataatc ttaccaaccg gcatattgag
    28741 atgagagcag atggactctg ccaatggtct attcgaacta cctgaaaaaa cagctaaatc
    28801 gtttatcata cagcaatctt tccttctgca atataacttt ctaatctttg cagatcttct
    28861 ggcgaattta ctccagaact ttctagatgg ttatttaata ccagcgcacc cgttttttcg
    28921 ttttttaaat ttgcaatttt aactaaatct ggtaaataat attcaccttg cgcgttttca
    28981 gtgttcactt tctttaaatt ttgaaaaact tctggagagt taaaaatata agttcctgta
    29041 tttatttcct taacatttct ctcttcatcg gttgcatctt tctcttctac aattcttaat
    29101 aattgatttg cattatttcg aatcagcctt ccataacctt tcggcgtatc aatttctgca
    29161 gataatacag ttacactatg atggtttgta agatgaaagt ttgctagatt tttaaaacta
    29221 gtagaagtta ataacggaac atctccacaa gctactaata aatttccttg aaagtctttt
    29281 aatgaattct cgcagcacag aagggcatga cctgttccaa gttgttcttt ttgttccaca
    29341 aaatgtatgt ttgtatagga tttacataaa tccattacta cttctttttt aaagccaaca
    29401 accacataaa tgtcttttat accagaagcg attaagttat cgagaacatg gagtaggagg
    29461 ggtttcccat ttaaaagagt agccactttg ggaagttcag atttcattct ggttcctttg
    29521 cctgctgcta aaacaacggc acaaatttta tctgtctggc tcattctttt ttattccgat
    29581 ttttcagtaa caaattttgc tgggctgcta ggattcgaac ctagggaatg gctggaccaa
    29641 aaccagctgc cttaccactt ggctacagcc caattttaga agctgaattg aataaagtag
    29701 tgttttggat agttttgagt catctttttc aagatgtccg ttccatggtc tctatcctgc
    29761 acaaacccat acacacagga acctgtccct gtcatagata cataactgca tcctgacttg
    29821 tataggcttt gcttcagcat ttccagttca gaaaattcgc taaaagcata cctttcaaaa
    29881 tcattttcaa atctatcttg gagttttgcc caatctccct gtgcaagtgc aacggtagag
    29941 gcttcatcca agagattcca tgatttttgt acccagtcag tttgtaaagg ctttttcaag
    30001 cttaagtaag attcttttgt actaattgaa aaaggtggga tagccaagat accaaaacca
    30061 gatgcaactt gaattggttt taaaacttca ccaataccag agaccagaaa gtggccaggt
    30121 cttagaaaga aaggaatatc tgccccaatt ttgggagcaa aatccatgat tagatttctc
    30181 ttctcattag atagtttggt tgcaagaaag gtaagtaagg aagcagcatt agaacttccg
    30241 ccacctactc caccacctgg ggggatacgc tttgttaaat ggacatcaat acccagatta
    30301 gagtcaaggt gagtcttgac tttttcgaat gttttaaata atatattctt ggtaaaatcc
    30361 ccacgctcag agacttcctc gaaaagatta tatttaattc cagataactc attttgcgaa
    30421 acaagccgta tcttaccaga atcattctct acaaattcta tatcatcacc ccaattaagt
    30481 tttaaaaata cactttctag ttcatgatat tgatcaggtc ttttatataa gatttttaga
    30541 cctaaattaa ttttagcagg agataacata cattagttgc ttggaatgat tttcggaatg
    30601 ttgatttgat cgatatgcga aagcacttct gctcttaaat tttcaggaga aagaattata
    30661 attctatttc caaatccctt gatcatatct aagaaccaat ttttctctaa tatctttgtc
    30721 tttgcataaa taaactgatt attaccattt attttattgc taataatttc aagttttgca
    30781 tatttagaaa gattaaactc aatttccttg gaaaataaaa tatgcgccat atctgacttt
    30841 tgctctgaaa catttaaaaa ttgattgaat tctaatatat gcttattttg ttcagatgca
    30901 tcaaggtaat gattaatctg ttgttctaaa acttgtggat tcgaaataga tgataagcga
    30961 aaatttcttc tgccgagtct gtcattacaa taggcaacta aatattcatt cttctcgctg
    31021 aatacaaacc agggatcaac agttctcagc tctggatttt cattcttcca gcctgcataa
    31081 taaaaagaaa gctttctttt cttttggatc gcttcctcaa ttttgctttt tatattttca
    31141 tgtgcattgg cttcactata tggtataata cgttttattt tatcaagtat agatttatat
    31201 tgagacttgt aagaatcttc tacttcagga tttgataatt cttcctctaa aattttacgt
    31261 atagcaagcc attcattgat tgtgagacct atcgttttat ctaaactaac cggcaaattt
    31321 atattaagtg tttcattttc gttaaaatct atttcgatat aatcggcagg agtataagga
    31381 taagagccta ccataaacaa ttcaccaaga gccttcttta attggtcatt atttttatag
    31441 tgagtataag atcgcaattt ggaaagagaa attccaggat tctcggtaat aatcttaatc
    31501 aggtttagtt tgtttgagac ccgaaatttt gtaggattca ttcattttga ataaatccta
    31561 gtgtttctaa tttggcaaga aataattttt catgctgcaa tactgaatga ttctttccga
    31621 tcattctctt cattttgagc gtaaattcta tccctcaaaa cctccatcga tctgcgatag
    31681 agtcttcctg tttggtgcct agaaattgcc aataaatcac tgatttcttt tattgaatac
    31741 aaaatataag tatccaataa ggaatttatg atgcgttgct tcctagctct gtagatatgg
    31801 gttgagctat ttgaagaatt aataattcta tgattgtatt tatttaaagt aagatttaaa
    31861 tcttctcgct tcgcacgcat tcgatgaatc ctatcctcat attcctgatg aaaaacttct
    31921 ataggctttg tcatgttttt atatagaatt ttcaaatcct ctatttccaa cttcaaatta
    31981 aatctgagac taacaatgat tcgccccata ctacaaagtt ctttcaagtg catccgaatc
    32041 ctatgcgtgt tatccaatat atgcggaatg taatctcgaa tcatcatacc ctctgaccac
    32101 aaaccaagat attcttcatt ataaatttgt tttcttcttt tataagtgct aaatgctaag
    32161 tgctttgtat aaacacttaa ataagctgga aaacttatat aatgatttcg ttcaaagaat
    32221 tcaatacact tctgcccatt ttcatagaag tgtataaaaa cttcagaaag ttcatcttca
    32281 tctagtctat atcgatatcg cgctttattc attatccaaa gtccagcggt atcgagtaat
    32341 aattttatat ttctttcctg cttatattta ttaaatgctg cttctagttg atcatttgaa
    32401 atttttggta gcatacaagt atccctttag gtaaatattt ttacacagga tgaaaggata
    32461 tatggtagcg tctaactttt tagaattaag gatattacgc agtttctaca gattttgtag
    32521 gtaatgcgta atttttttaa tttttttttc taactcaatg tagaaaaaat atatttttac
    32581 tttctatcaa ttttattttt tagatcttct gtattttcaa caaactgaat agatgatact
    32641 ttatactcag aaaggctaat cactgttagc cttcctttct cacgagggaa aataaagcct
    32701 ggtccttctt ctctaatgag tgcaatcgaa tatggatatt tacgcatttt tggaagggca
    32761 acaaactttg taattaaaaa aggcatttta tgaatatcag aaataaaaat tgcattgttt
    32821 tgctttagat aatcatctcc tagtttttca aataactcat gagcaatttt actcgcctcc
    32881 atatcagaag taaagattag agttcttgtt tctttcgata tgataatctt ttcttctttc
    32941 tgagtttcaa actgcaagag agtcatttca tctcctactt ttaatggttc acttaataat
    33001 tggaggctaa tggcaaatat aaaaaaaaat atttttttca tgaagacaaa tctaaaatga
    33061 aatcaagtgt tagcaagaga tttaatttgc tgttctcaat tatctcctat ttttaattcc
    33121 atttcgatta aattaattcg gaatccaaat acatcttcct gtatagttct aatgggatga
    33181 aagcctattt ttttataaaa attgaaggct ttctgatttt tctcttctac atgcaagagg
    33241 aacgacttac attttctttc tctgacatct actaataacg ctttgaatat tctctctccg
    33301 aatccttttc gctgaaattc tggtaaaata taaagtcttc ctaactgaat cagattttct
    33361 tctacttcaa atgcagttcc tattcctatc aattgatttt gtatagaccc aactttaaaa
    33421 atcaccttct cgttttgaat ttgtctagac aaaacttcaa cgttatgcca ctcatgcgta
    33481 attttattta tagtttcttg ataaaaaaaa tctgaatagg tatgcttcca agtagtagat
    33541 agaatattct taattgcatc catattctct atactggcct tttcgattgt taagttcata
    33601 catcaccctc cttttgaaat attataccaa acgtcaaaag aaaagtccca ttttaaaaat
    33661 agaaatgtgg gtttggtcta aacatttctt agtgttcccg aaaatcagta agttcggtat
    33721 accaaatgct attcaatgaa aatccttcgg ggaaattact tttctcattt ggtatataaa
    33781 agtctttaaa ttaattctag ctagttttac cagtcaaaaa acttaagatt tttaaagatt
    33841 ttaatgttaa aaatcaattt aggctaaatc attttcaaat tcttttcata agcctaaagc
    33901 aaaaaagcaa taggaatgag ttagaatcat ttcgcatatc gcattaaagc actccatctg
    33961 ccaagcaatg ccagatagct aacagggatt taccgccccc acgaagtctc cgactaagga
    34021 gacggaggtg tgcttgattt tttgcttagc aaaaaagggg cggaatttcc cgtgaaaatg
    34081 tcttgcagac ctcagcccct tccgcctgat ggctaacggg gcttcgtcaa tcttgtatga
    34141 cagccattga gtattcggag atggcttctc ttctttgttc tgcaaagtcc ttgtgaaacc
    34201 aaaggtcttg tgtcttctga gcagactttc ggttacttaa aataatctta gtcatacact
    34261 cttctactgc aaacttcatt gcttcttttc ctgtttctcc gagtcctttg tttctcttct
    34321 ttctaagcat ctcaccgcca tttttacgaa tacggtccat tagcttatcg tagtccttgt
    34381 catcgataca gaagatagtt tcttttttcc cgtctttcca cttaacgtct acttcaaaca
    34441 atggagcaga agatatatga atcactccaa aatcaaaaag ttcaggccag tattcataga
    34501 aaaaagaaag cataagagat cggattgcat atccatcaaa gtcggcatcc gttatgatac
    34561 ttatcttttc atagtttaat tcttttgcat ctttcaactt ttcattgatc ggcaaaccaa
    34621 gaatagccac aatgttcttt aactcttcat ttgcaagagc cttagcaaga ctcattcctt
    34681 tacagttcat gggtttacca cgtaaaggga atagcccatg taattttgga tttctagcag
    34741 gacgaagacc agcaatagca gaatcacctt cagcgataaa tagaacgcgc cccgcatcac
    34801 ttggcttacc ggtaggcggc ataagctttg gaatattatt tttagatgct tttcttaagc
    34861 ctttttgagc atcttccaac tcttttagct gagtcctctt ctccatttgc atttttactt
    34921 cttcgagaag gtctgttttt ttgatcagct tgtctaggtt tttatccaca gcgtttcgaa
    34981 tatcttcatt taaatcatta atcaaataag atttatcctg cgatttaaaa cgtggattta
    35041 aaattcgtag atttacatac atatgaaagc aatttcgcac atcgtttcga gtagattgag
    35101 tttttagttt tttctcaagt gatacgattt gacttttctt tcttacctca tcacagattc
    35161 ggttttctaa atattcaatt gcggaaccac cttgcactgc aaagtttgaa ttcacccaag
    35221 ttaaagtttt attttgtcca attacaaaat aggtttccag atgaatttgg gaagctgagt
    35281 ttggatcttt gtaatccatt ttaaaatagg ttaaatctga attgataaaa atctcttcca
    35341 ttcccttttt aaatttaaac ttctcagatt tatttttatg atgaaactga acttcgagtc
    35401 cagggtttgt catagcgata tcttgtagat actgacgcat taaatttgga ttgaatttcg
    35461 tatctaattt attaaaatac ttcggctcta attcaaattc aactgctgtc ccatgcgaat
    35521 ccccgcaagg cttaatagtt tcgatcataa aagtttttgc catcaaaggt tctaatttag
    35581 atagatgatc tttatttaaa agtggacatt ctttaaagtt accatgctca tcataataaa
    35641 gaataatttt ttcaaactct tcaggtgaaa acttaaaact tcgtaacagt tttcgaactt
    35701 catcatgaac agtaaaaagt tttttataag actgaccttt gttttcagtc gtaactctaa
    35761 agaaattaga aaccattcta acaagggaaa tcccaacccc attttgccca gcaacatggt
    35821 cttgcttcac ttggtcatca aagttttccc catacatcaa atgaagaaaa acaccttcgg
    35881 catttttggc tggaattcct ctcccgttgt cctctacagt aactcttcca caatcagcag
    35941 agagactaac gattaattta gtcatctttt cgccatcttt tagagcttta tcattttgat
    36001 ttttacggta ttcgtctacc gcattcatac aagcttcatc taagcatttt aattttgcag
    36061 gtatatcatt taactcttca tgagaaattt cataatttcc cttagaatcc ttcttaaaaa
    36121 aatgctgttc aaatgtagac atggagtttt gtccaagcca catacccgtt ctcatacgaa
    36181 cgtgttctac gttagataat tttttaaaat ttctttcgcc tgattctttt gtaggttttt
    36241 cttttatatt agcactcatt atttatccca cttgtttcta agttcagtta attcatcaat
    36301 cataaaagcc gtaagtttgt tatcttgctt tgatagagca gtaaattcag caaactttgt
    36361 ctttgcttcc gcaatagctt cttggcattt ctttacttct tctaacgtca tacgatagac
    36421 aggaattgat gcaagccatt caaaatattt gaatttagca cttttaagtt gattttcgaa
    36481 ttcttgttta gatttaatat tagttacttt ttgattccat ttttcattga taaaacggat
    36541 taactcagaa tttctatcaa ttttttcttg ctctagtcca gctagcctct taaatctgcg
    36601 gataaggtgt gttttgcgaa aatcgcagaa tctcttaata atttcctctg gtttaaaatt
    36661 ttttaagcga ccttcatgtg ttattacgtt aaacgaaaga gtatctaagt tttctttgtt
    36721 aaaaagagct ttaatatctt tctctgtcgg cttttcccct tttttataaa caagttcgat
    36781 tctaaaagtc tggctcgaag aatcgatgta atcctttaac caattgtctt ttctttcgat
    36841 catttcttcg agtaaggcaa tgaccttatc tcgattccat gtttggggtg catgtgtaag
    36901 aaataaactt tcaccttccc atttaaaggc aaaggttgta tacataacgt ccgaaccatt
    36961 ttcattcgta acgactctta cttctccgtt atagtcttta taccatggtt taattttctt
    37021 aggcttacct gtttttaaat aagcaacctg tgactcgata atatcggaaa gtttatgtcc
    37081 aggaatgaaa catctaaaac ctgtcgcaat tccctgaatc tgatttagca atacaatagg
    37141 aacttttcca acaaaatgaa ttggctcatc ttcggtctca tcataattct ttacataatc
    37201 aatatccgaa agtccctcaa agaatcctaa gtctttagca aaatcagaaa gcttcacttc
    37261 tgtatatcga ggagaggcaa ttgcatttgg atcaagaaca tcaccgaatg ttccttcccc
    37321 gtgaacgagt ggaatattat tcgcaaaagt aaaatcctgt gccattgcgg aaagtgcatc
    37381 ctgaatggac ttgtctccgt gaggatgata ccccatcgca aggccggcaa cttttacagt
    37441 cttagtatgt ctgtttctgg cgtcggaatt ccacattgcc catagaattc ttctttgaac
    37501 tggttttaat ccgtctattt catgcggaat agcgcgagaa tcgcatacat aacgagaata
    37561 ttttctctgg tcatcgttta cttggtcttc aaataatacc tttggaaagt cggttcgagt
    37621 tgtttcttgt gtatttttca tcttcttctt gttttgcggt agtaagtctg tctattctgc
    37681 ttgtcaagca ttttcataat tttacattgg cttaacaaat aaaaggcctc tttttcattt
    37741 tt
//
LOCUS       DAOQOS010000047        36987 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_461_length_36987_cov_6.369950, whole genome shotgun sequence.
ACCESSION   DAOQOS010000047 DAOQOS010000000
VERSION     DAOQOS010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 36987)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 36987)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36987
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_461_length_36987_cov_6.369950"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..309
                     /locus_tag="PLF66_11985"
     CDS             <1..309
                     /locus_tag="PLF66_11985"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HMZ64809.1"
                     /translation="LGAGNFFGEMALLTGAIRTADIIALTQTRLIEITRSDFEPLLKA
                     HPEIFQLVDKVSHSRKADTETQKILSQLPPEPVKDPWYKVYLKKVRHFFMELIKRPTE
                     "
     gene            complement(441..1247)
                     /locus_tag="PLF66_11990"
     CDS             complement(441..1247)
                     /locus_tag="PLF66_11990"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HMZ64810.1"
                     /translation="MKDIKNKVTLVTGASQGIGRAIATSLALAGANVAIIASRKEEVE
                     KTAAEIRALGGKCEGFACNLMDTELFFQVHDQIVAKMGPIEILVNNVGAGTFKPMHLM
                     TRKEAMLPVQLPFGIAVAASHAVIPSMLEKKSGHIVNLISPAGIFPLPYMMPYTAARY
                     AMLGLSHSLYEELHHKGIGVTMVCPAQVNTNYFQKNDADMGWYPKISKVFPVLEPKEV
                     GDAVVEGILKDKREVILPFFLRALIFFYRPIPRFWVGLFKLLGLWGPSNK"
     gene            complement(1640..2020)
                     /locus_tag="PLF66_11995"
     CDS             complement(1640..2020)
                     /locus_tag="PLF66_11995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010410511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04452 family lipoprotein"
                     /protein_id="HMZ64811.1"
                     /translation="MKKLILSILSIYIFSQCVILNPIGLNSDRIKGTEAASKIRSAAI
                     TQDLLLSVAFLGRPIISILSILADDIAGIDGSKYYLESEVNSCISKIRGFEGFLLGAG
                     LTVAASCKLKPADGYITGKPLPKL"
     gene            complement(2573..2962)
                     /gene="queF"
                     /locus_tag="PLF66_12000"
     CDS             complement(2573..2962)
                     /gene="queF"
                     /locus_tag="PLF66_12000"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016753021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HMZ64812.1"
                     /translation="MKKKISSYEGKQTHIRDLKTPTIETFTNIYKGSDYTIDFAIPEF
                     TAICPKTGLPDFGTIYITYTPNSECVELKSLKEYILFFRNVGIFHENVVNKIFEDLMK
                     SIKPKYLKVFGDFSVRGGIKTTVKRES"
     gene            3000..4802
                     /gene="guaA"
                     /locus_tag="PLF66_12005"
     CDS             3000..4802
                     /gene="guaA"
                     /locus_tag="PLF66_12005"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004770725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HMZ64813.1"
                     /translation="MIHSKKIGVIDFGGQYAHLISSRVRRLGAYTEILSNEESLEKYK
                     EYTGLILSGGPNSVYEKDSPQLDEKVLHMGIPILGICYGHQLIMKLLGGKVESSLNRE
                     YGPAFLEMEEFPAIVKGFSEKEKIWMSHGDEVVSLPEGFHPFGRTDHCRYAGVYHTEK
                     KIYGIQFHPEVTHSEKGMLFLENFISICGLSNTWGMDQFLEEKISEIKKETKDNNVFM
                     LISGGVDSTVSYLLLAKALGGERVKGLLIDTGFMRKNEVSNLSEKLHSLGVDLLVSDE
                     SERYYEALKGKSDPEEKRKIIGQLFIDSKDAIAVKYHLTDEKWLLGQGTIYPDTIESG
                     GTKHSHKIKTHHNRVQGILDLMEKGRIIEPIRDLYKDEVRELGTRLGLERNWVNRHPF
                     PGPGLAVRMLSSNEYHGFSNNEGLTSVISKFDKLQAKVLPIQSVGVQGDQRSYSACAV
                     LTDCLSDWDKYEEASTQITNLVKGVNRVVLLPFVKDFTQMEFRFSKIELDRKHSDLLR
                     EIDDVVWNHLVEHKLVEAIWQMPVVLVPVGASVGKFSVVLRPVESQEAMTANFYRMNR
                     DQLRSLCDKLSRFNELSYVFYDITNKPPGTIEWE"
     gene            complement(5610..5963)
                     /locus_tag="PLF66_12010"
     CDS             complement(5610..5963)
                     /locus_tag="PLF66_12010"
                     /inference="COORDINATES: protein motif:HMM:NF020397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HMZ64814.1"
                     /translation="MKENSKIKDFKNYTKTLPSSTIKVLEEIRKIVKAQVPKAEETIS
                     YQMPAFKLKKVFFYYAGFKNHIGIYPPIKKDKKLLKELAPFAGPKGNLKFPLDKPIPF
                     SLIERVAICLAKEYE"
     gene            6219..6407
                     /locus_tag="PLF66_12015"
     CDS             6219..6407
                     /locus_tag="PLF66_12015"
                     /inference="COORDINATES: protein motif:HMM:NF014664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HMZ64815.1"
                     /translation="MEFQKFNSIAPNIVKALRDMTQSVDATGLEKTLTELIKIRVSQI
                     NGCAYCLQFHINLARKSG"
     gene            6507..6656
                     /locus_tag="PLF66_12020"
     CDS             6507..6656
                     /locus_tag="PLF66_12020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64816.1"
                     /translation="MLLEEIPESKYDKLSLYFSQQEVVYLTSSIATINAWNRIAESLK
                     FLVPA"
     gene            6953..>10481
                     /locus_tag="PLF66_12025"
     CDS             6953..>10481
                     /locus_tag="PLF66_12025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64817.1"
                     /translation="MQSSLYHSILVRITTVKRIFQAKLNLLEQLKIVAHNNFHRKKVR
                     ERLLVKAYLQAEELASHSKRFSFESILKKAPIWIQNEMNRQEKKRKRHFKSISKSTRI
                     LLPTSILSKFKIQEFFHLAISLEKTFEKIVLIPILIIALVLEKLGFRVFEKRLVRFLS
                     KGFRLWITVICYLAVFQSLRAVDFDDLKETSPGFFGREKIYGSEKEKRYAIDGFFIEW
                     ERWPRTNPTHSSFHLFWFLNQVNYPKYQRSQLFPLYLSESSKVDQRYEANHLLLSNYS
                     KEKDGSYAYRLFPFLWFGNGAGKSHHGVFPLYYKSKMDTANQKEDFLVMPFYFGSSES
                     TSDEEKTFSLSPLHFNSNTKAKQISENRFFAPIVPLYYSYSDEAKIQRNYLGFIDTEK
                     DKKSDSISRVWVMPVFYWREKSYLNVFPLFFRNVAEDEKSGAWYGLAPPVYYSYSPLH
                     STFYLLNYYSTSNQTKEKSETFTAFAPFYFQTSDNTGYSQILIPGLYYSETNADKSTH
                     KNITLLYDHIYDKKEKLDRLFIAPFLFYKKDSYLNIAPFYFSSLSKSEEYRNWYSFIP
                     PIYYSRSPTEKIFYILNYYSSSISIDKNSSDFTTIAPVYFNWSNNKGYNETIVPFIYY
                     SKTKEDSSSYKNVFLIYSQVNDHRGKLNQILVSPIFFYKRDSYFHFAPLYWNWRSDEY
                     DETSKITQADNYLLFPFFYHESKKRASYTNILGLVSREYDHKGKLAGSMFAPFYFYKK
                     DSYEIIFPFLLKKGMDNTDKKGTQMGLFYYNDWNSDEETLLAGNYYSHKDIKDNSYFR
                     TLFPIYYEWKNKKWSGEVTPFYLTLDFPNRDRFHLNASGYSYSLNVGTIESDKKLGKF
                     HRYVDADYSWLGYAVRISTRTTFDFLEEGQAKETVDTLDITPSLSKKKSFTRENSKQF
                     YGVNLLFGLYGWEMGDTKRHIRAFPLTWLTWDTRSNDKVITAPPIFVHYSSEDLRYTV
                     LFPFYGNQKTKESERTSIALIGYIDEEIQDKNIKEKSILWPLINWHSSDLKTSSRFIP
                     FYWHRKQKEEIGLVTNFITPFFYHNQTVEKNGNEVANTFSPLWFRFESKDQKDSLRQH
                     FTIPIYYYRENISSVDRSELFLSLPYNSYTEIDGTSKKPIRKTIVILPTYYNKEYAKT
                     ANDTENTKFYSPLYIYNGESNQASKKEDFS"
     assembly_gap    10482..10581
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <10582..11904
                     /locus_tag="PLF66_12030"
     CDS             <10582..11904
                     /locus_tag="PLF66_12030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64818.1"
                     /translation="ARVFPLYSHAISKDKTGSVTNVTRWIFPTYYEKNYAKTANDTEN
                     TKFYSPLYIYNGESNQASKKEDFSWFLPIPFLYHSYETDNDRTYWNWLAFVGYEDAKT
                     FNHARVFPLYSHAISKDVTGSVTSVTRWILPTYYEKNYAKTSNDTENIKFYSPLYIYN
                     RESNQASKKEDFSWFLPIPFLYHSYETANERNYWNWLGFAQYENAKNKKSILAFPVLY
                     MKEENKKYSALIVGLYLDSNAKQSYLNFLLLTENEHNKETEERKVNLLLRTLQFAKSK
                     EKFNFKLLYGAGGVEKSSDVTDVHLLWLEHENKKDSLYNNFLPIYYNKDTGNEKRSWF
                     TPTLSYSKKDTKEKLNVIGLGSLYYNKEDLEKKEDHMNILMGALYYNNSIPKERGYKE
                     HGSLWGLLWEYKSESETKYSKISVLKFVYSKTTDETGESYQRIMGVRL"
     gene            12203..12946
                     /locus_tag="PLF66_12035"
     CDS             12203..12946
                     /locus_tag="PLF66_12035"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HMZ64819.1"
                     /translation="MAAKEHYDKHLGSFYSWMVGDFESKQNEFSEFLNEMKILPRSSK
                     NVIDLGAGHGIQTISLAKLGFHVTAIDFNEQLLQELKKNSEGFDIKIVNDDIKNISKY
                     SNLNPELVVCYGDTISHLASLAEINKIITDIFLVLDRGGKILLSFRDYSKELIRDDRF
                     IHVKSDDSRILTCVLDYTIDKVLVTDLLFEKIDNTWIQKVSSYEKVRISSNSIVELLE
                     ATGFKIERNLVWNRLIHILAAKPLDSKID"
     gene            13356..14843
                     /locus_tag="PLF66_12040"
     CDS             13356..14843
                     /locus_tag="PLF66_12040"
                     /inference="COORDINATES: protein motif:HMM:TIGR00813.1"
                     /note="Members of the Solute:Sodium Symporter (SSS), TC
                     2.A.21 as described in tcdb.org, catalyze solute:Na+
                     symport. Known solutes for members of the family include
                     sugars, amino acids, nucleosides, inositols, vitamins,
                     urea or anions, depending on the system; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/solute symporter"
                     /protein_id="HMZ64820.1"
                     /translation="MESFFAFSDASVHPLDISLIVSYLFGILVFGIYVGKGVKNSSDF
                     FLAGKSLAWWVVGLSIIGSNIGSNDYVGAAGSAYKIGIAQANFEWIGAIPAMILAAFL
                     FIPYYWKAGVYSIPEYLGLRYNQSVRLISASVLSVFTVLMVGAFLWSTALMLKTYLGW
                     PIYFSILVSAFVVGVYTATGGLKAVTITDTVQVFIMFAGAFGLMFLGIEKSGGFNAFI
                     TKINTEYPEHLKAFLPATHSEFPWPGVVLGLGLVLSPAYWCTSQVILQRTLGAKSQWD
                     GQASMIFAAFAKTLVPFLIIFPGFLALVLVKTPLEYPDQALPWVVKNVLPPGLSGLLF
                     VSFIAALQSSVDSTLNSTATMVTRDIVGVIRKERDDSFDLKLGKIVTTVALLLGMIVA
                     PFTSLFAGIYAFIQEMLSLFQGPMFALVLWGILSRRPTSKAGLYTIVFGLLFAFGLNR
                     TGVNMLYVAFYSFIGSSIILFVSSLFTEKKTTTELKNLTIHEVEK"
     gene            14840..15049
                     /locus_tag="PLF66_12045"
     CDS             14840..15049
                     /locus_tag="PLF66_12045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64821.1"
                     /translation="MKHFLQDFWATLPEWYAKPSAALLFILLLILIWKLPVERIYEEC
                     PSFWKDLRIWSSILLVVQMVIYFIF"
     gene            15342..16400
                     /locus_tag="PLF66_12050"
     CDS             15342..16400
                     /locus_tag="PLF66_12050"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HMZ64822.1"
                     /translation="MDLEIIHKTFYGNTILEWCIAFSIIVGALVLSKVIYWFFKHAAR
                     SLTQKTKTNIDDIIVDMMEEPVSLAIVLTGIYFGVGMLNITSGIEGFKGKVFQFLFIL
                     NIAWAIERLVNSFFENYLVPLSAKTENDLDDIVIPIVQKGVAFTIWSIGVVVGLNNAG
                     YDVGAILAGLGIGGLAFAIAAKDTISNMFGGLTVFIDQPFSIQDRVKVKGIDGRVTDI
                     GLRTTKLITMEGRRVTIPNSAFIDNPVENISSEPTRKIVQTLKLAHTNNYTKIEEAIL
                     ILKNILDRNPKVEKESFIVSLTGIEETGFILQVIYYIIPKASKEIFDTQTEVNQEILS
                     QFEQNWIKFAYNVLPKTT"
     gene            16397..17395
                     /locus_tag="PLF66_12055"
     CDS             16397..17395
                     /locus_tag="PLF66_12055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008592148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3089 domain-containing protein"
                     /protein_id="HMZ64823.1"
                     /translation="MKYFTLFIFLISGCAFLVKPEKTIEKNDTLILPDYSNLSFWAAH
                     PEKKDNADLVPDSSLSDRQEFASADVFYIHPTTLIQAKYWNGDLTDSVLNDRTDEGTI
                     KPQASIFNDCCKVYAPRYRQAAIYAFLKETKEGKQALDFAYEDVKKAFEYYARNLNKG
                     RPWILAGHSQGALHSIRLLKEVISNSDIGKSMVAAYVLGWPFRGEEIGFPICKSQEDI
                     RCVIGWNTYVWGNYPSQRKELYNGAVCVNPLSWKADNEYMPKEKNLGSLPKGFNRIIP
                     TLADAKCEDGILWVNSVSSEGFPTLEFGKNYHLVDFHLFYMNIRENSKLRIKSFPY"
     gene            17552..19258
                     /locus_tag="PLF66_12060"
     CDS             17552..19258
                     /locus_tag="PLF66_12060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64824.1"
                     /translation="MRVKSKILLGILLLMYTINNCKSENKEGLNPLTLIALIFATRSS
                     GGIVNVSGTITYEHIPVNTSTKTLDTTGKTERPARGITVRVLDSSSNTVASTTTDSSG
                     KYTITGANVSSSTFTVAAVAEMVKTSSPTYNFKVVNTVASGSIGTTYAVASASTSVSS
                     CSSSCTVNVTALDSNRGNGPFSILDVVYIAIQKVLTADSNTTFPPLNLRWTSTSTTGS
                     FFTTSSSTCGSGVSNCITLLGNRSGDSDEFDRHVIAHEFTHYLESALSRSDSVGGSHS
                     TNDTLEPRVAYGEGLGNAMGAIFLDDPIYTDTTASGGFNLNMETGTHTNVGYYSEASV
                     QSVIWDLYDSASDSKNSQTDNLNYSFTKIWNAVVALKSINGITYLHEFILALKNANAS
                     DTTSINSILTMETIAATEAGEGNVSKSSAGTSNAHICTGSGASTGYPYNFIIESPGGT
                     TGTVINAVSISGSQNCGITSYANNKLFGSKFYRVTPANSGTMTVIATDGGGTTEDPDV
                     YIFQQGTSVKTCNQGISENCQTTVTAGKVYIIEIRTYSTCAVTACGTGNTNSATITIT
                     LP"
     gene            19268..19792
                     /locus_tag="PLF66_12065"
     CDS             19268..19792
                     /locus_tag="PLF66_12065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64825.1"
                     /translation="MFIQFIKFIFILILATSCSLNSNHSANSKVFYSDKQNPHAKMQL
                     PLMISWSHDKADNANQPISVQIAANALSDLTNVTIKLTLSPDLKLVSGETSKTISTMS
                     AGSTTELNLTVIPLKEGSFDINILLNGLMNGESIGSARTIRLDTSSNNTKKTEEQPDP
                     SGLYIIPGKTEYGK"
     gene            complement(19834..20955)
                     /locus_tag="PLF66_12070"
     CDS             complement(19834..20955)
                     /locus_tag="PLF66_12070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010569617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HMZ64826.1"
                     /translation="MSRVSYFDYNATHPPFTKIVSEAYTEYSVNFYNPSGATRFSLSR
                     QSIIESARSYFSEITGKDKKQFVFCSTGTEANTLLVASLVDYLKQFKRVFISPFEHSS
                     FYGPLDFYGIQYELIQTNKTGLIDLEDLSSKMKEDPAPIFMIYAANETGVIQPMSDIH
                     SIAKEYKMPIFSDLMQAYGKININYSLLDGFTFSGHKIGAGMGASLVYSPESYLKKEF
                     GIFKGGNQENNHRAGSENSAAIQAFELVSKEQFKTLSEKNLRLTAYRNQIEKVLTKLG
                     AEIIAENSPRLPSTTFTLLPIDDIDFFMLGMEEANLVVSTGSSCKSRTRDPSFSLLKM
                     GYSKEEALRAIRISTGYFTTQDEVDRLIEVMTQLIKKLK"
     gene            complement(20952..22310)
                     /locus_tag="PLF66_12075"
     CDS             complement(20952..22310)
                     /locus_tag="PLF66_12075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64827.1"
                     /translation="MIKILKDNLVYLLLLFLIWAIAFPIVISNRDSLFSWYAISMSPS
                     GYSPKVARKFIEKGDKALDKNLDDAIVSPRIKEELEDEKKRPAILLSHLELMEEACDY
                     YKKIAGKDEVLQYPSWLDRNTKWSSDRELSADGIGKSRGHLPTVLNPEEYWKKNVDSV
                     LGALDYYKRALNYSGPEILAADKIRSVAKAVCRPEEIILAYTSYITVSDAYVEKILEE
                     EDRQFAKKNQSFFSPILRLFGMAGPAERKEMYGLNPLMKRSIIWSQIRKNIKEGIKGG
                     SGEDPNLPLLSDYIRGLNILLLDTNIRNSSPKEADALYETILFFLQGTTDPGQQKNER
                     FFRFKRGILLFNMGDYERAKEQFTAAKEFEDLKDESPVSMRLIASHIFQSNLMIAKCN
                     FKLGNYKEALIALKSVENLLANVDGRNGGKIEIDLLTDYKNTLRETLKKLDRFKEADE
                     IE"
     gene            22751..24721
                     /locus_tag="PLF66_12080"
     CDS             22751..24721
                     /locus_tag="PLF66_12080"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HMZ64828.1"
                     /translation="MKKVLFVIMLFFSIGIWADEGARCKPLAGETEYYSPVFLLSMGI
                     KMQSQANHANSKNPQQVVSLHKKAIQYFHAYIQCAKENAIPVNSSTYYLKATSHFEIR
                     EWNECSTDLDTSLALSNNQYKDALILKSRLLIKQNKFKQAADLLESVISFYSEDSDLL
                     YFLGSLSAEVENYPKAILYFTSLYNLIQKKEGDAKYRNFILKYLGDIYSKKGDGQRAI
                     HYYKLYLKYKENDPEVFFTLAQIYITLGDFNESRIYLNKILKTNPDSKPVIQLLAEMH
                     FIENKMFSLSYFENLHKEGKFKEEGLLASIYKVLVGKHKEAEEYFVTMSSKNPGRLSL
                     RMALIIIYSKLKKTPELASELKITSEIAYANKQYNLAIELAMQLLDISLNNPDLKIKP
                     VLLYDFIATCYEDSGAPFLGIHYLRKALDTLESDSERINLTLHMANIYRNSKVKRYDE
                     SNKILQPILKSENPNAYFIQGMNYFSMEQYKESIESFSNAIAIDAKNPNYYFFRASAY
                     EKNKQLNETIQDLKKSLELDNTNAATYNFLGYLYAENNMELVDAYNFIKKAVELEPDN
                     GSYQDSLGWVLFKMEKLKESLHHLNLALQIMTDRKEDDPVVYDHLGDVFFKMNDMDNA
                     SENWKKSVEIQSSPMEKEKILQKIKKLGSSKS"
     gene            24737..25540
                     /locus_tag="PLF66_12085"
     CDS             24737..25540
                     /locus_tag="PLF66_12085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64829.1"
                     /translation="MKKTISILFLFSLILLGCKTEEGSVKEPEYTDGKQTQNLKYNDP
                     RAKSLVEIVERLEKNSNSFTGEFTMKILSGDKLKESNTVNGKIFFDKPTGKVKIQLME
                     PFFGLIISQIISDNSRIRIKSSGQEKIHEQAMGDIYMVDPNTRKTIVIPYPIIYFSIA
                     QNFIEEFRSDKSYLSPSDKRVLVKRGTDEYKYFFYDKGLSSLELFAPDKNIRAICQIP
                     EKYRTGDHPPERISTRVTDATTELDTSQVDIHYKNVKRNATIPPKTFEF"
     gene            26157..29780
                     /locus_tag="PLF66_12090"
     CDS             26157..29780
                     /locus_tag="PLF66_12090"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HMZ64830.1"
                     /translation="MDTYHISPYPELTSNIDSIQYFWHMLPYAVPGFITFFCGLILAL
                     IGFFRVFSRKEKTGYYLSFSGMCLAFGMLGFVLALRAVIHDTEILLRWNTVLYTFILL
                     YAPSSAHLIYYILDKKYKIALVGAGINWIATLFAFYGIVTNQAFTGNFLQYSFGIYPI
                     ASIYLKAWGIIGAATFFFLGVPTYAHYLKRNSFYEKRFLVIGHILSILLVISNLPSFM
                     GIPLFPGSTFAFIPMLLLAYGIFLTDFSDLRVMLFEKNGFFYVLNLFASLVLLLIAIA
                     VIFFFSPLQYQYQSKSLLTWQLIPLISVLLVVGLGIFIGGSNPHKPLNQMGAFSLYLY
                     GFQLLASISIGLSPDPMVGHRIEQLCFLLFCFAPSIQSRFAFLSFEKKIPKFFILIDI
                     ASFLLAVLALSPYLFVGYYNFPWGRFSASGPVIHGLGMVGFIAMIFTLNEWNQKRKRK
                     EIKPIGNFAVFYLATGAIMLLFNIPATMGIPLYPLGNLSIFPTMILVYAIVRYGESTI
                     NTESFRISSYLVPFALGIVIFILCLVWVNIPREVSFESRLFHLFISGVPLILLSFLST
                     FILIRPIAARVDKTLESLRREKEITEKQKMEIEIINDYSKKLNSKLDLDKILDEIFEY
                     FDDRYSIEGIIIQFLDKEKNEFYSYKTTTPENAAQEIIDYSQNLRLPFDERLGILFSA
                     YKKLRTLYVPRVVYNLQPPIIQDMINALRFQNFLFVPLIIQDEVIGSMFFTSYRKRID
                     LSKTDISNISIFCNQISTSIQNSKLFKQVKEEKEKALEAKLEAEKAKFEVEILGDFAK
                     LVNSENILDHIFSYAVINLIQNLNADIFWLQLVDKNKNELYTRCISNSGNVPEVINKF
                     KAMRIPLSAQSGSIYLTYKRKKILYISDISKTRSMLSEIDKSLINEFNLKSAFQIPLL
                     INDEVIGILHINKLGGMKKLTQDDIRSTEALCEQLGIAINKTALYEKAELERRKSENL
                     LLNILPKDVASELKEKGAADPVQFDSVSVLFTDFQGFTTIAERMKPNDLIKELDACFI
                     QFDRITERCNLEKLKTIGDSYMCAGGIPRSNKTHAIDCVLAAMQMQDFMKRTREEKTE
                     QGVPYWEIRLGIHSGPLVAGVIGEKKFAYDVWGDTVNTASRMESSGHPGKINISGSTY
                     ELIRDYFECEHRGRVKAKNKGEIDMYFVLRLKPEFSDDEEGKIPNQKFKEIYNQKNRF
                     YPI"
     gene            complement(30222..32111)
                     /locus_tag="PLF66_12095"
     CDS             complement(30222..32111)
                     /locus_tag="PLF66_12095"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HMZ64831.1"
                     /translation="MALDGEWDFYWNQFLTFHDIMESVPLKSLKENENLIQIPGNWKG
                     KLISKRPLAGDGYATFHLHVKLLAGTNDMALRIPVMGTANKVWIDDKLIFEAGKIGKT
                     KEEMTAYLMPQNHLLNLENKHEFHITAQISNFMDRTGGVWGSFVLGTVENIYKQKFVT
                     ISLEMMVFGGLFIMSIYHFGLFYTRTRDKSTFFFGFFCLNFAIRTIVTEERILMSTFP
                     FLSFDFTYRLEYICGYIAFPLFVSFMNYLFEEEINKRYLKIIWIFFSFLCLTVLLLPH
                     YEYTHLLIFFEIHVFLSIPYLLYVNFSAIFKKRDGALSSFIGIIILAIGAVIDILHNE
                     HIVFWHYVTPFTLFGFLFFQSFSLSTKFSKAFQISETQSIELREQKNTLSAINDELTT
                     LQRELEHKVKLRTDELEIIYKQNLNEIQKVNALEKEIAIQKERQELFVDIHDNLGGKL
                     LILSLQLKSIEPDKTISKNLKARIVKTIDEILKGLRNHLLAFEDISYIEKNFNKGFKN
                     FLVRRYSTAERKIQLDVDSNVSIHAIPKDYYHNLFSILQEIINNDLKYGRDKTKWLVK
                     NSEDSILFQMHAHSEFNNIPWKAGNGHKTIQKRVKLIGGIFFEKLENDFYFCELKINA
                     SKVNL"
     gene            32981..34330
                     /locus_tag="PLF66_12100"
     CDS             32981..34330
                     /locus_tag="PLF66_12100"
                     /inference="COORDINATES: protein motif:HMM:NF019237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HMZ64832.1"
                     /translation="MQRGKKDNGPLLLGAAALANQIRNNSGAGSSGANANTTVVTPTI
                     TSPAAGQYSTSQTITITTTTSDATIYYTVDGTDPTTSSNVYTQAISIWSLAGQSRTIK
                     AIGVKSGFSNNSIATQTVGVFNYIPHKTGQTLCWDAVGNPTTCTGTKHDGDLQNGITR
                     SYTGPTQNSTYTSDYTSTDNVTGLVWKTCSQGLSGATCASGTIAPMNWQDAQTGSNGC
                     SSLNALNSGNGYAGKKDWRLPTRQEVETLTDRGRNNPAIDPTPFPATSGSYWTSTAYG
                     LNTANAWTVQYNAGSMNNVVKTDNTVNVRCVSGLSRGYIGSFTDNGNGTIKDNVTGLI
                     WQKCSMGQNNDATCSGTATSRSWANALADCNGLSLASRTWRLPNVNEINSIVDTTKTS
                     NPTIDATFFPSTSSIYWSSSTVAIALSSAWILPFGSGGNVTDQNKNNTYNVRCVSGP"
     gene            complement(34523..35797)
                     /locus_tag="PLF66_12105"
     CDS             complement(34523..35797)
                     /locus_tag="PLF66_12105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002972945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HMZ64833.1"
                     /translation="MTLVRKILNLVKSFLKSKFFKSSIYVSLSKVFSSICNLLFMIFA
                     VNILNKAENGIFQYYLGYLPVILAVAEFGLPSAIVKFISSVSDNKEKIGVILSSSLLI
                     KIISFLLLLALGFGAYTFYAEDPLIIFILLIGGTTISFVSFFESIFVSFQAYKALAFW
                     NPLANVMRLILLYLTHKYSELPLTYLDILAIFSLSPIFILILFFFIFNRNKIYWGGKL
                     KEITHVTKELSLFNTWAFVASIFAIISDRLEIFLINKYHSSEQVAVYGTALQLFSGFV
                     IILSTLNSLIYPRLSALVHTDEFKNFLLKSVFAGFAIAILLSPGYLLAEPILNLLFNH
                     KYEEAIPVFKILYPNYMLQLVFAPLGIALFAMGKPRILAILALLRLVFGYILDTALIP
                     EFGVLGAGTAFLLGQVISWLVLVGYFWAMFWK"
     gene            complement(35851..36834)
                     /locus_tag="PLF66_12110"
     CDS             complement(35851..36834)
                     /locus_tag="PLF66_12110"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HMZ64834.1"
                     /translation="MNEHHVLFENGYLFGNLFLEAIPHPTGTIGDILRWLSTLCHFMG
                     GANFFVFLLPFVYLCYNRNFGIKLGVALLSTAFFNGLAKYMFESARPIDLPDSILAVQ
                     SDLIKETSFGFPSGHSHVSILVWGILFLEFKNKFFRAFALFVILFTPFSRMYAGVHYP
                     GDVIGGFSMGLISLILIEFLFYKIPDFPRIEFNEDSKNRIVRSISLFILALTLSSTLL
                     PKGITHPQISSLEQVLIGSGSIAGFSIGLILLKGLYSEASEWQVVSSFKDFLVRAGFL
                     LPGILVFYLGLGILGKTFHIEESLFRYLRYLLTNLYIVLLAPLFWYRFRSK"
BASE COUNT        12339 a         5924 c         6586 g        12038 t
ORIGIN      
        1 cttggagcgg gaaatttctt tggggaaatg gctctactta caggtgcgat tcgaactgca
       61 gatatcattg ctcttaccca aacaaggcta atcgaaatta cccgcagtga ttttgagcca
      121 cttctaaagg cacacccaga aattttccaa ctggtagata aagtttccca ttctagaaaa
      181 gcggatacag agacacaaaa gattttgagt caattgccgc ctgaaccggt gaaagatcct
      241 tggtataaag tttatctaaa gaaagtaaga catttcttta tggagctcat taaacgacca
      301 actgagtagt gaattcgatg taaaaatttt ttaaccacgg atagacacgg ataaacacgg
      361 atgtggattt tttatcagtg tttatacatt cgaaagactc agtgcaagtc ggtggtaatc
      421 ttttttttat cgaattgacc ttatttattg cttggtcccc agagtcctag tagtttgaaa
      481 agtccaaccc agaagcgagg aattggtctg tagaaaaata taagtgctcg tagaaagaaa
      541 ggaagtatga cttctctttt gtcttttaaa attccttcta cgactgcatc tccaacttct
      601 tttggttcta gaacaggaaa tacttttgaa attttaggat accatcccat atccgcatca
      661 tttttttgaa aatagtttgt atttacctgt gcggggcaaa ccattgttac accaattcct
      721 ttgtgatgca attcttcata caaagaatga gagagtccta gcatcgcata acgtgcagct
      781 gtatatggca tcatataagg gagtgggaaa attccagcag ggctgattag atttacaata
      841 tgacccgatt ttttttctag catagaagga atgactgcgt gagaagcggc aaccgcaatg
      901 ccgaatggaa gttgaacggg aagcatggct tcttttcttg tcatcaagtg cattggttta
      961 aaagttccag ctccaacgtt gtttactaaa atttcaatgg gacccatctt tgctacaatc
     1021 tgatcatgca cttggaaaaa aagttctgta tccattaggt tgcaagcaaa tccttcacat
     1081 ttaccaccta acgctcttat ttctgcagcg gttttttcta cttcttcctt tctacttgca
     1141 ataattgcga catttgctcc tgctagtgca agactcgttg cgattgctct tcctattcct
     1201 tgagaagcac ccgttacgag tgtaacttta ttttttatat ctttcatatt ctctccctat
     1261 tcggtaagat ttgtaattag gaatcatttc aacaagtaaa attctgattc cactgtgagg
     1321 atagaactct tatgaaaatt tataaattct taaaaggatt agaaataaga ttcaaagaat
     1381 ggatagaaag aagattgttt tgttactata aaaaattaca tagtaaaaga gggttttgat
     1441 tttatttcag tcggggaatg gattaaagtt tggatttaag gctgaatgtt tctgtctaga
     1501 aatttggaaa gttagtaggt cggtgaaaaa atgaaaccta tgcgttttca atctaatcta
     1561 gttgagtgat tctattgtat ttcaatcaat ttgaaatata tcgcacaggt tttatgaatt
     1621 ggcaaaatcc tttgtagaat tataattttg gcagaggttt tcctgtgata tatccatctg
     1681 caggttttaa tttacaagat gctgctacag tgagtcctgc ccctaataaa aatccctcaa
     1741 aacctctaat cttgctaata catgaattta cttcactctc caaatagtat ttacttccat
     1801 caattccggc tatatcatct gcaagtatag aaagaataga aattatgggt ctacccaaga
     1861 atgcaacact caatagtaaa tcttgtgtga tcgcagcaga cctaatttta ctagctgctt
     1921 ctgttccttt tattctatca gaattcaaac caatcggatt taaaatcaca cattggctaa
     1981 agatatagat tgatagaatt gatagtatta attttttcat agtttgtcct caaattttat
     2041 taaaaccaga ttactgattt ctttagtagg ttgtcagtct tatttgagaa tcttttctta
     2101 taaatgcact aagaaacctg ttttttattt tagaggtgag atttttttgt tggaatccta
     2161 aagatttaac cttaaaattt accggctaat tccaattatc ctgtaatgag taggattgtt
     2221 gctgataggt aaatggaaga atctaggtgt agatgataga agagaatacg agcgatagaa
     2281 taaaaatatt ttttgtatcg ttcgactttt atagagcaaa aagcgatggg tgaaaagtca
     2341 tttattccct ttgctcgagc ctattgccat tgttcaatga caataggata tcgcacacca
     2401 gaacgttttg ctctcctaaa ctctatcatt gattttccaa atagaataga agatctattc
     2461 agaatcgttt gatacaggaa agtttttctt gtccgaattt aatatccaag ggaacaaaat
     2521 ctttttgttt tcctattcat ttggatcatt gtaaaaaatc agacttggtt ttttaagatt
     2581 ctctttttac agtagtctta attccacctc ttacactaaa atcaccaaat acttttaaat
     2641 acttaggttt gatcgatttc attaagtctt caaagatttt atttactaca ttttcatgga
     2701 aaattcctac atttctaaag aacagaatgt attcttttaa agattttaat tcaacacatt
     2761 cagaattggg agtataggtg atatagatag ttccaaaatc tggaagtccc gttttaggac
     2821 agattgcagt aaattctgga attgcaaaat caattgtata atcactcccc ttataaatat
     2881 tagtaaatgt ttcaattgta ggggttttta aatcgcgaat atgagtttgt tttccctcat
     2941 aggaagatat tttttttttc atggttcctc taactttttc aatgattttt aggtaattta
     3001 tgattcatag taaaaaaatt ggcgttatag atttcggcgg acagtatgca catttaattt
     3061 cttctagagt gagaagactt ggggcatata cagaaatttt atctaatgaa gagagtttag
     3121 aaaaatacaa agaatacacg ggtcttattt tatcgggagg accaaatagt gtttatgaaa
     3181 aggattctcc acagctcgat gaaaaagtct tacacatggg aattccaatt ctaggaatct
     3241 gctatggtca tcaattgatt atgaaacttc ttgggggaaa agttgagtct tctctcaatc
     3301 gagagtatgg acctgctttt ttagaaatgg aagagtttcc ggctatcgtt aaaggtttct
     3361 cagaaaaaga aaaaatttgg atgagtcatg gagatgaagt tgtttcttta ccagaaggat
     3421 ttcatccctt tgggcggaca gatcattgtc gttatgcggg agtttatcat acagaaaaaa
     3481 aaatctatgg aatccaattt cacccagaag taacacattc agaaaaagga atgctgtttt
     3541 tggaaaattt tatttctatc tgtgggttat cgaatacttg gggaatggat caatttttag
     3601 aagaaaaaat atccgaaata aaaaaagaaa caaaagacaa taacgttttt atgcttatat
     3661 ccggtggtgt tgattctact gtttcttatt tacttcttgc aaaagctttg ggtggagaaa
     3721 gagtaaaagg acttctgatt gatactggct ttatgcgaaa gaatgaagtt tctaatttat
     3781 ctgaaaagtt acattctttg ggagtagatt tacttgtttc cgatgaatcg gaaagatact
     3841 atgaagcttt aaaaggaaaa tcagatccgg aggagaaacg aaaaatcatc ggtcagctat
     3901 ttatagattc aaaagatgcg atagctgtta agtatcacct aacggatgaa aaatggcttc
     3961 ttgggcaagg aacaatttat ccagatacaa ttgaaagtgg tggaaccaaa cattcccata
     4021 aaattaaaac ccatcacaac cgagtgcagg gaattttaga tttaatggaa aaaggtagaa
     4081 tcattgaacc aatacgagat ttatacaaag atgaagtaag agagttagga actagacttg
     4141 gactcgaaag aaattgggta aatcgacatc catttcctgg acctgggctt gctgttcgaa
     4201 tgctttcttc caatgaatac catggattct ctaataatga ggggctaaca agtgttatct
     4261 ctaaatttga taaactacaa gcaaaagttc ttccaattca atccgttgga gtgcagggag
     4321 accaaagaag ttatagtgct tgcgctgttc taacggattg cctttcggat tgggataaat
     4381 atgaggaagc ctctactcaa ataacaaact tagtaaaagg tgtaaaccgt gtagtattac
     4441 ttccttttgt aaaagatttt actcaaatgg agtttcgttt ttctaagatt gaattggatc
     4501 gtaaacattc cgatttattg cgtgaaattg atgatgtggt ttggaatcat ttagtagagc
     4561 ataagctcgt agaggcaatt tggcaaatgc ctgttgtttt agttccagtc ggtgcaagtg
     4621 ttggaaaatt cagtgtagtg cttagacccg ttgaatcgca agaagcaatg acagcaaatt
     4681 tctatcgaat gaatcgtgat caattgcgat cgttatgcga caaactcagt cgattcaatg
     4741 aattatctta tgtgttttat gatatcacta acaagccacc cggaacgata gagtgggagt
     4801 aatgacttag gtttggcaga attcaactcg ttcaaaaaaa ggaattttta ccaaacgtca
     4861 aaagaaaagt cccatttcaa aaatgaaaaa gcgggtttgg tctaaacact tcttagtgtt
     4921 cccgaacatc agtatgttcg gtataccaaa tgctactcaa ataaagtcct tgtggaaaat
     4981 tacttttgtc atttggtata taaaattttg acaaacgatg gaaataaaat attaatcgta
     5041 tcttcttccc attggattaa gaatttacca tgataagata caatattggg atattaaaat
     5101 agtcaaaaaa actattttat aactcacctt atattatcat tgcttaacaa aagcaatgat
     5161 tgagtttgtg tgagccgagt gttcaaatga ggtttgctcc tagcggaggg cttgccaaac
     5221 tcaatgtttc tccctaaggg tcgaaacata aggcaggcta ttgattttat tagtttatta
     5281 gcattctcta atattttaaa aatcatcttt tgtggctcaa tttattaatt ttgtgtaagg
     5341 tgagttagta agcaaagagg gtgagggcgt ttacattgaa gtgataccgg aaatgatgat
     5401 tttcaagctt tagacaagaa aaaatttaca aaaacatatc ctccgcatta ccctgttgaa
     5461 agtgaagtta atttacggtt aaaagaatca gaatcaaaac agtaagccaa acaattttca
     5521 caaataaaaa aagtttatct cttgtctttc accccaactt aatctgcctt tccaattgaa
     5581 ggataacacc gatttgccta attctttcct tattcatatt ctttcgctag acatatcgct
     5641 acacgttcga tcagagaaaa gggaattggt ttatctaatg gaaattttaa atttccttta
     5701 ggacctgcga atggtgcaag ttcttttaat aattttttat ccttttttat aggtggataa
     5761 attcctatgt ggtttttaaa tccggcataa tagaaaaaaa ctttttttag tttaaaggcg
     5821 ggcatttgat aactaatcgt ttcctctgcc ttaggaactt gtgcttttac aatttttctg
     5881 atctcttcta atactttaat tgtagaagag gggagagtct tagtataatt tttaaaatct
     5941 tttatctttg aattttcttt cataattgga tgtcttattg aaggaattcg atactctcat
     6001 tgtcaataga tttatttttg gatgtgatat aaatcaattg attccattgt gaatcaaaaa
     6061 aagaaaggaa tatatattaa atgactgatg ttaagcaaaa tttataattt aaaggataaa
     6121 tgatattttt taaaatatta gagaatgtta atgaattaat aaaattaata gatataacaa
     6181 aaagaaaatt aaaaatatta ataaagaagg atttctacat ggaatttcaa aaatttaatt
     6241 cgattgcacc caatatcgta aaagcattgc gtgatatgac acagagtgtt gatgcaacgg
     6301 gcttggaaaa aactttgaca gaactcataa aaattcgtgt ttctcagata aacggttgcg
     6361 cctattgtct tcaatttcat attaacctcg ctcgtaaatc tggttgagag gaaaataaaa
     6421 tagaacgtgt ttctcgatgg aaagaggcag atattttttc agagagagaa caagctgcac
     6481 tggattgggc agaaagtctg acgaaaatgt tattagaaga aatacctgag tccaagtatg
     6541 ataaattaag tttatatttt tctcagcaag aagttgttta tctaacctct tccattgcta
     6601 caatcaatgc ttggaatcgg attgcagaga gtttaaaatt tctggtccct gcataaaata
     6661 tttggctgaa aaatctaatt cgatgttgaa taaacactgt ttcactaacc cggaatcgtt
     6721 tctatctgtt taaaaaattt atattgatta taaaatttaa gtatgtttgt ctattttttt
     6781 acaaaaaatg aacaaacttt tttatatttt aaaaaaaaat gcaaaaatta aaaactatgt
     6841 ttgtctaagc ttttgtgtaa caaaaaagga aaagaaataa agaatatttt tagatttatt
     6901 ctaaatttcg tttctaggtt caaagagtaa tattagaaaa aggtaaatag aaatgcaatc
     6961 aagtctttat cattctattc tagtgagaat aactactgtt aaacgaatct ttcaggcaaa
     7021 actaaatctt ttagaacaat taaaaatcgt tgcacataac aattttcata ggaagaaagt
     7081 cagggagaga ttacttgtta aggcatacct acaagcggaa gaattagcat ctcacagtaa
     7141 acgtttctct ttcgaatcta ttttaaaaaa agcgcctatc tggattcaga atgaaatgaa
     7201 tcgtcaggaa aagaaacgaa aaaggcactt caaatccatc tctaaatcaa ctcgaattct
     7261 tttacccact tctattttat ctaaattcaa aatacaagag ttttttcatt tagccatatc
     7321 attagagaaa acctttgaaa aaatagtttt aattccaatt ttaatcattg ctttagtgct
     7381 agagaaatta ggttttcgtg tattcgaaaa aagattagtt cgatttctat ccaaaggatt
     7441 tcgcttgtgg ataaccgtta tttgctacct agcagttttt caatctttaa gagccgttga
     7501 ctttgatgat ctaaaagaaa cttcacctgg tttttttgga agagagaaaa tctatggtag
     7561 tgaaaaagaa aaaagatacg cgattgatgg tttttttatt gagtgggaaa gatggccgcg
     7621 aacaaaccct acacatagct ccttccattt attttggttt ctgaatcagg taaattaccc
     7681 taagtatcag cgcagtcagc tcttcccact ctatctttct gaatctagca aagtggatca
     7741 acgctatgag gcaaatcatt tattactatc aaattatagt aaagaaaagg atggatctta
     7801 cgcatatcgt ttatttcctt tcttatggtt tggaaatggt gcaggaaaat ctcatcatgg
     7861 tgttttccct ttgtattata agtcaaaaat ggatacagca aatcagaaag aagatttttt
     7921 agtgatgcca ttttattttg gttcctctga atccacttcc gatgaggaaa aaacattctc
     7981 attatcaccc ttacatttta acagcaatac aaaagcaaag cagatatctg aaaatagatt
     8041 ttttgcgcct atcgttcctc tttattattc ctattcagat gaagcaaaga ttcagcgaaa
     8101 ttatctaggt tttattgata cagaaaaaga taagaagagc gattctattt caagagtttg
     8161 ggtgatgcct gttttttatt ggcgagaaaa atcatattta aatgtctttc ctttgttttt
     8221 tagaaatgta gcagaagatg aaaagtcagg agcttggtat ggtcttgccc ctcctgttta
     8281 ttactcctat tctcctctcc acagcacctt ctaccttctt aactactact ctacatcaaa
     8341 tcaaaccaaa gaaaaatctg aaacctttac cgcctttgct ccattctatt ttcaaacatc
     8401 ggataatact ggttattcgc aaatattaat acccggattg tattattctg aaacaaatgc
     8461 agacaaatcg acccataaaa atattacctt attgtatgat catatatatg ataaaaagga
     8521 aaaactagat cgacttttta tagctccttt tttattttat aaaaaggata gttatctaaa
     8581 tattgctccc ttttatttta gttctctttc taaatcagaa gaatatagaa attggtatag
     8641 ttttatacca ccaatttatt acagtagatc tccaacggag aaaatctttt atatactaaa
     8701 ttattactcc tcttctattt ccattgataa aaattcttca gacttcacta caatcgctcc
     8761 tgtctacttt aattggtcaa ataataaagg ttataatgaa acgatagttc cattcatcta
     8821 ttactcaaaa acaaaagaag attcatcaag ttataaaaat gtctttttaa tttattctca
     8881 ggtcaacgat catcggggga aactaaatca aattttagtt tcccccattt ttttctataa
     8941 aagagattct tactttcatt ttgctccact ctattggaat tggcgatcag acgagtatga
     9001 tgaaacttca aaaataactc aagcagacaa ttatttatta tttccctttt tctatcatga
     9061 aagtaaaaaa agagcaagct atacaaatat tttaggactt gtgagtcggg aatatgatca
     9121 caagggaaaa ttagcaggat ccatgtttgc tcctttttat ttttataaga aagattccta
     9181 tgaaattata tttccttttc tactcaaaaa aggaatggac aatacagata aaaaaggaac
     9241 tcagatgggg cttttttatt ataatgactg gaactcggat gaagaaacgt tacttgcagg
     9301 aaactattat tctcacaaag atataaaaga taattcttac tttcgaaccc tatttccaat
     9361 ttattatgaa tggaaaaata agaagtggtc tggggaggta acaccgtttt acctcactct
     9421 tgattttcca aatagagatc gatttcattt aaatgcctct ggttactctt atagtctgaa
     9481 tgttggaacg atagaatcag ataaaaagct aggcaaattt catcgatatg tagatgcaga
     9541 ttatagttgg ttgggttatg cagttagaat ttctactaga actacttttg actttttaga
     9601 agaagggcaa gcaaaggaaa ctgtggatac tcttgatata acgccaagtc tatcaaaaaa
     9661 gaaatctttt actagagaaa attcaaaaca attttatgga gtgaatctac tctttggtct
     9721 ctatggttgg gaaatgggag atactaaaag gcatataaga gcatttccac ttacatggct
     9781 tacttgggat accagatcaa atgacaaagt gataaccgct cctccaatct ttgttcatta
     9841 ttcttctgaa gatttacgat atacggtatt atttccattt tatggaaatc aaaaaacaaa
     9901 agagtccgag agaacgtcaa tcgctctcat tggatatatt gacgaagaaa ttcaagataa
     9961 aaacataaaa gaaaaatcaa ttttatggcc gcttatcaat tggcattcat cagatttaaa
    10021 aactagttct cgatttattc ctttttattg gcatcgaaaa caaaaggaag agattggact
    10081 tgttacaaac tttattacac cattctttta tcacaatcaa acagtcgaga aaaatggaaa
    10141 tgaagtcgca aatacgttct ctcctctttg gtttcgattt gaatcgaagg atcaaaaaga
    10201 ttctttgcgc cagcatttta caattccaat ttactattat agagaaaata tttcgtctgt
    10261 cgatcgatca gagctttttc tttctctccc atataattcg tatactgaaa tagatggcac
    10321 tagcaagaaa ccaatccgta aaacaattgt gattctacca acctattaca acaaagaata
    10381 tgcaaagact gcgaatgata ctgaaaacac caagttctat tctccccttt atatttacaa
    10441 tggggaatca aaccaagcaa gtaagaaaga ggatttcagt tnnnnnnnnn nnnnnnnnnn
    10501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10561 nnnnnnnnnn nnnnnnnnnn ngcaagagtc tttcctcttt attcacatgc aatttcgaaa
    10621 gataaaactg ggagtgtaac aaatgttacc cgctggatct ttccaactta ctatgaaaaa
    10681 aattatgcta agactgcgaa tgatactgaa aacaccaagt tctattctcc gctttatatt
    10741 tacaatgggg aatcaaacca agcaagtaag aaagaggatt tcagttggtt tctaccaatt
    10801 ccatttcttt atcattcgta tgaaacagac aatgatagaa cgtattggaa ttggctagca
    10861 tttgtcggct atgaggatgc aaagactttc aatcacgcaa gagtctttcc tctttattca
    10921 catgcaattt cgaaagatgt aacaggaagt gtaacaagtg ttacccgctg gatcttgcca
    10981 acttactatg agaaaaatta tgcaaagact tcgaatgata cagaaaacat caagttctat
    11041 tctccgcttt atatttacaa tagggaatca aaccaagcaa gtaagaaaga ggatttcagt
    11101 tggtttctac caattccatt tctttatcat tcgtatgaaa ctgcaaatga gagaaactat
    11161 tggaactggt taggatttgc gcaatacgaa aatgcgaaga ataaaaaatc tattttggca
    11221 tttccagttc tatatatgaa agaagagaat aaaaaatatt ctgcactgat cgttggattg
    11281 tatttagata gtaatgcaaa acaatcctat ctaaatttct tactattgac agagaatgaa
    11341 cataataagg agacagagga aagaaaagta aatcttttgc tgagaacgtt acagtttgct
    11401 aagagtaagg agaaatttaa tttcaaactt ttatatggag caggtggggt ggaaaaaagt
    11461 tctgatgtga ctgacgttca cttactatgg ttagagcacg aaaacaaaaa ggactcatta
    11521 tataataatt tcctaccaat ctattataat aaagatactg gaaatgaaaa gagatcctgg
    11581 tttactccaa ctttgtctta ttcaaaaaaa gatacgaaag aaaaattaaa tgtcattgga
    11641 cttggatctc tttactataa caaagaggat ttagaaaaga aagaagacca tatgaatatt
    11701 cttatgggtg ctttgtatta caacaacagt attccaaagg agcgtggtta taaagagcat
    11761 ggaagtcttt ggggactttt atgggaatat aaatcagagt ctgaaaccaa atactctaaa
    11821 atttctgttt taaaatttgt atattctaaa acgacagatg aaactggcga gagttatcag
    11881 aggataatgg gggttaggct ttaaaaaaat aggagaatag aatcagggaa aagtctttaa
    11941 ttttagaaaa aaactaagat atggaaatgt ggaagaggaa aaaggattgt tgaacttgta
    12001 attttcccag attcagattg tttacttgtt ctagactagt tgagagaaag gatttagatc
    12061 attgggatta atggaaagta tcttgctgtt atagacagga ctccaatttt ggttataaag
    12121 acttttttct gaaatgccaa ttgagggtcg attcttcagt cgagttgagt aataagaaaa
    12181 aggaaaattt attggagaga aaatggcagc gaaagaacat tatgataaac atcttggtag
    12241 tttttattct tggatggttg gtgattttga gtcaaaacaa aatgaatttt cagaatttct
    12301 aaatgaaatg aaaatacttc ctagatcctc aaaaaacgtt atagatcttg gggcagggca
    12361 cggcatacaa actatatctc tagcgaagct aggttttcat gtaacagcaa tcgattttaa
    12421 tgaacaactt cttcaagagc taaagaaaaa ttcagaaggg tttgatatta aaatagtaaa
    12481 tgatgacatt aagaatattt caaagtattc aaatttaaat cctgaattag ttgtttgcta
    12541 tggtgatact atatcgcatc tcgctagtct tgcggaaata aataaaatca taacagatat
    12601 atttttagtc ttagatcgtg gcggtaaaat tcttctctct tttagagatt attctaagga
    12661 attgattcgg gatgatcgtt ttatacatgt aaaaagtgac gatagtagaa tactaacctg
    12721 tgttctagat tatacaatag ataaagtttt agtgactgat ttattatttg aaaaaataga
    12781 caatacttgg attcaaaaag ttagctccta tgaaaaagtt agaatctcct ccaattcgat
    12841 tgtagaatta ctagaagcca caggatttaa aattgaacga aatttagttt ggaacagatt
    12901 gattcatatc ttagcagcta aacctctaga ttcaaaaata gactgatata ttatatcaaa
    12961 tgacaaaatt aatttccgag aaagattatc attgattcgc atttggtata ccgaaggaca
    13021 ctaagaagtg attctaaact gggagattac tcttgacgtt ctctataagc agaaaagttt
    13081 ataactagta tttttagtga gcgatttaaa atttaaaaac tagaatcatc taagtcaata
    13141 attcaagtag aacttatcat catcgaaaca ataggcttgt tgttttctaa attcgagcag
    13201 ttatttttat gttgcatgag acaggactga gtttataaaa cctcaatatg aatctaaatt
    13261 tgaaatacaa caatctagaa aaatttactt actaaattta aatctatttg actcggttgg
    13321 cttttatctc cttagttgac tgaaaaggaa atcttatgga atcatttttt gcattcagtg
    13381 atgcttccgt gcatcccctc gatataagtt taattgtttc gtatttattt ggaatcttag
    13441 tttttggaat ctatgtaggg aaaggagtta aaaattccag tgactttttt ctagcaggaa
    13501 aaagtttagc ttggtgggta gtgggtcttt ctatcattgg atctaatata gggtctaacg
    13561 actatgtagg ggctgctggg agcgcatata aaattggaat tgctcaggca aattttgaat
    13621 ggattggagc aattcctgct atgatccttg cggctttttt atttattccg tattattgga
    13681 aggcaggtgt ctattcgatt ccagaatatc tgggtctgcg atacaatcaa agtgttcgat
    13741 tgatttcagc cagtgtatta tctgttttta ctgttttaat ggttggggct tttttatggt
    13801 cgactgctct gatgttaaaa acctatctag gatggccaat ctattttagt attttggttt
    13861 ctgcttttgt agtcggagtc tatacggcaa caggaggatt gaaagcagta acgataaccg
    13921 acacagttca ggtttttatt atgtttgcag gtgcattcgg acttatgttt ttagggattg
    13981 aaaaatcagg cgggtttaat gcttttatta ctaagattaa cactgagtat ccagaacatt
    14041 taaaagcatt tcttcctgca acacattctg aatttccttg gcccggagta gttttgggac
    14101 taggtcttgt tttgtcccca gcttattggt gcacaagtca ggttatttta caaagaacgt
    14161 taggagcaaa gtctcaatgg gatgggcagg cgtctatgat atttgcagcg tttgcaaaaa
    14221 ctcttgttcc atttctaatc atatttccag gatttctagc tcttgtatta gtaaaaaccc
    14281 ctctcgagta tccggatcaa gctttacctt gggtggttaa gaacgttttg cctcccggac
    14341 tttctgggct tttatttgtt tcctttattg ctgctttgca atcttcagtg gattctactt
    14401 taaattcgac cgcaactatg gttacaagag acattgtagg agtgatacga aaagaaagag
    14461 acgattcttt tgatttaaaa ttaggaaaaa ttgtaacgac agtagctctt ttattaggaa
    14521 tgattgttgc accttttaca tctctgtttg cgggaatcta tgctttcatt caagaaatgc
    14581 tttctctatt tcagggtcct atgtttgcgt tagttttatg ggggatctta agtagacgtc
    14641 ctacttctaa ggctggattg tatacaattg tatttggact tctatttgca tttggtctga
    14701 accggacagg tgtaaatatg ctctatgttg cgttttatag ttttattgga tctagtatca
    14761 ttttatttgt ttctagttta ttcactgaaa agaaaacgac cacagaatta aaaaatctta
    14821 ccattcatga ggtagagaaa tgaaacattt tttacaagac ttttgggcta ctctacctga
    14881 gtggtatgca aaaccgtcag ccgctctatt atttatactc cttttgattt tgatatggaa
    14941 attgccagtg gaacggattt acgaagaatg ccctagcttt tggaaggatt tacgaatttg
    15001 gtctagcatt ttacttgttg ttcaaatggt gatctacttt attttttagt aattaatttg
    15061 aatttgaggt aaaaaaattt tcaccaccga ttcacaccga tacctgaacg atgaagacgc
    15121 gatggataat tttgaatcat tgtatctttt atcgatgata atcgtcctta caaattcaac
    15181 atggctcagt gcaagtcggt ggtaatcttt taattttccg ttacatggaa ctggcggtaa
    15241 ttaattgaga gagaatctct atttatctaa atattgggat tatgaagtaa aaataaaaaa
    15301 cttggttgaa tggaatctcc ctttcaaatt tcaatcctag tatggactta gaaataattc
    15361 acaaaacatt ttatgggaat acgattttgg aatggtgcat tgcattttcg attatcgtag
    15421 gtgctcttgt attatctaaa gttatttatt ggttttttaa gcatgcagct cgtagtctca
    15481 ctcaaaagac aaagacaaat atcgatgata tcattgtaga tatgatggaa gagccagtat
    15541 ctctagctat agttttaact ggaatctatt ttggagtggg gatgttaaac ataacgtccg
    15601 gtatagaagg ttttaaggga aaagtttttc aatttttatt tattttaaat attgcgtggg
    15661 caattgaaag attggttaat tctttttttg aaaactattt agttccgctt tctgctaaaa
    15721 cagaaaatga tctagatgat atagtgattc caattgtgca aaagggagta gcatttacta
    15781 tttggtcgat aggcgttgta gtaggtttaa ataatgctgg ctatgatgta ggagcgatac
    15841 ttgcaggact tggaattgga ggtcttgcgt ttgccattgc tgcaaaagac acgatttcca
    15901 atatgtttgg tggacttacc gtttttattg atcaaccgtt tagtatccaa gatagagtaa
    15961 aggtaaaagg aatagatggg agagtaacgg atataggtct tagaactaca aagctaatta
    16021 ccatggaagg cagaagagtc acaattccaa attctgcttt tatagataat cctgttgaaa
    16081 atatttcttc tgagccaact agaaaaattg tgcaaactct caagctagct catacaaata
    16141 attatactaa aatagaagaa gcgatattaa ttctaaagaa tattttagat cgaaatccca
    16201 aagtagaaaa ggagtctttt attgttagtt taactggaat tgaggagact ggatttatac
    16261 ttcaggttat ttattatatt attccaaagg cttccaagga aatatttgat acacaaacag
    16321 aagtaaacca agagatcctt tcccaattcg aacaaaattg gattaagttt gcatataacg
    16381 ttcttccaaa aactacatga aatattttac tctttttatt ttcttaattt cgggttgtgc
    16441 ttttttagta aagcccgaaa agacgataga aaagaatgat acactcattc tcccagatta
    16501 ttctaatttg agtttttggg cagcacatcc tgaaaaaaag gacaatgcag acttagttcc
    16561 cgattcttct ttatcggata gacaagaatt tgcttccgct gatgtttttt atattcatcc
    16621 aacaacattg attcaggcaa aatattggaa tggagattta acggattcag ttttaaatga
    16681 tcgaacagat gagggaacca ttaaaccgca agcgagtatc tttaatgact gttgtaaagt
    16741 ttatgcaccc agatatagac aggcagcgat ttatgctttt ttaaaagaga caaaagaagg
    16801 caaacaggca ttagactttg catatgagga tgtaaaaaaa gcttttgagt attatgcaag
    16861 gaatttgaat aaaggaagac cttggatttt agcaggacat agtcaaggag cacttcattc
    16921 tattcgtctt ttaaaagaag tgatttcgaa ttcagatatt ggtaaatcaa tggtagccgc
    16981 ttatgtattg ggatggcctt ttcgtggaga agaaattggc tttcctatct gtaaatcgca
    17041 agaagacatt cgttgtgtaa ttggctggaa tacatatgta tggggaaatt atccgagtca
    17101 gcgtaaagaa ctctataacg gtgcagtttg tgtaaatccc ctcagttgga aagcggataa
    17161 tgagtatatg ccaaaagaaa aaaacttagg cagtcttcct aaggggttta atcgaattat
    17221 ccccacgctt gcagatgcca aatgtgaaga tggaatcctt tgggtcaatt cagtttcaag
    17281 cgaaggtttt ccgacattag agtttggtaa aaattatcat ttggtagatt ttcatttatt
    17341 ttatatgaat atcagagaaa attctaaatt gagaataaaa agttttcctt actaataaaa
    17401 acttgaactt catttgttga atatttagtt tggctcaaga ttacttttgg gtcatccatt
    17461 ttattctcca tactacatag gtttagagat taataagaga cattaaacaa gaaaattgaa
    17521 aggagttttg tagcatctgc tactagatca aatgagagtt aaatcaaaaa tattattggg
    17581 gattctttta ctaatgtata caatcaacaa ttgtaaaagt gaaaataaag aagggttgaa
    17641 tccattgact ctgattgcat taatctttgc tacccgaagt agtgggggaa ttgtaaatgt
    17701 aagtggaact ataacctacg agcatatacc tgtaaatact tcaactaaga ctttggatac
    17761 aactggaaaa acagaaagac cggcgagagg tataacggta agagtcttag atagtagttc
    17821 aaacactgtt gcatcaacaa caacagatag ctctggaaaa tacacgataa ccggtgcaaa
    17881 cgttagttct tctactttta ctgtagcggc tgtagctgaa atggtaaaaa ccagttctcc
    17941 aacctacaat ttcaaggttg taaatacggt agcctctggt tctattggaa ctacttatgc
    18001 tgtagcctca gcaagcacaa gtgtaagttc ttgttcatct tcttgcacag taaatgtgac
    18061 cgcactagat tcaaatagag gaaatggacc ttttagtatt ttagatgttg tatatatcgc
    18121 gattcagaaa gttttaactg cagattcaaa tacaaccttt cctcctttaa atcttcgatg
    18181 gacttctact agtacgacag gtagtttttt tactactagc tcttctacct gtggaagtgg
    18241 tgtgagtaat tgtattacac ttttagggaa tcgatcgggg gattcagatg aattcgaccg
    18301 tcatgtgata gcacatgaat ttactcatta tttagaatct gctctatcta gaagtgactc
    18361 tgtgggaggc tctcattcta caaatgatac tttagaacct cgtgttgctt atggggaagg
    18421 tttaggaaat gcgatgggcg caatattttt agatgatccc atttatacag atactaccgc
    18481 atctggtggt tttaatttga atatggaaac aggaacgcat acaaatgtcg gatattattc
    18541 agaagcatcc gttcaatctg taatctggga cttgtatgac agtgcttccg attctaaaaa
    18601 ttctcaaaca gataacttaa actactcttt tactaaaata tggaatgcag tggttgcatt
    18661 gaaaagtatc aatggaatta cgtatcttca tgaatttatt ctggctctaa aaaatgcgaa
    18721 tgcaagtgat acaacctcca ttaatagcat tttaactatg gaaacaattg ccgcaactga
    18781 agcgggtgag ggcaatgtaa gtaaaagttc agctggaacg tctaacgccc atatctgcac
    18841 aggctctgga gctagcaccg gttatccgta taattttatt atcgagtcac caggtggaac
    18901 gacagggact gtcatcaatg cagtgagtat atcaggaagt caaaattgtg gtataactag
    18961 ttacgcaaat aataaattat ttggaagtaa gttttataga gttactcctg ctaattcggg
    19021 aacgatgacc gttattgcta cagatggtgg tggaacgact gaagatccag atgtatatat
    19081 ttttcagcag ggaacaagcg ttaaaacttg taaccaaggt atttctgaaa attgtcaaac
    19141 gacagttact gcaggaaagg tatatattat tgaaattcga acctattcaa cttgcgcagt
    19201 caccgcttgt ggaacaggca atacaaattc agccacaatt actattacat taccataagg
    19261 atttactatg tttattcaat ttattaaatt tatttttata cttatacttg ctacttcttg
    19321 ttctttgaat tcaaatcatt cagcaaattc aaaagttttt tactcagata agcaaaaccc
    19381 tcacgccaaa atgcaacttc cattaatgat tagttggagt catgataaag cggataacgc
    19441 taatcagcca atctcagttc aaatagctgc aaatgcttta tccgatttaa caaatgttac
    19501 tattaaattg acgttatctc ctgatctaaa actagtatcg ggtgaaacgt ctaagacaat
    19561 ttcaactatg agcgcagggt ctactacaga attaaattta acggtgattc cgttaaaaga
    19621 gggatctttc gacataaaca tcttgttaaa tggacttatg aatggagaga gcattggaag
    19681 cgctagaacg attcgcctag atacaagttc taataatacc aaaaaaacag aagaacaacc
    19741 agatccatca ggcttatata ttattcctgg taagactgaa tatgggaaat aatattctac
    19801 aacccatatt taatttgtgt gatatcagtt aatttatttc aatttcttaa ttagctgggt
    19861 catgacttct atcaatcggt ctacttcatc ttgtgtagta aagtagccgg ttgagatgcg
    19921 tatcgcacgt aacgcttctt ctttagagta gcccattttt aaaagagaga agcttggatc
    19981 tctagttcta gatttacaag atgaaccagt agatactact aaatttgctt cttccatacc
    20041 aagcatgaag aaatcaatgt cgtctatcgg tagaagtgta aaggtagtag agggaagacg
    20101 aggtgagttt tctgctataa tttccgctcc taattttgtg aggacttttt caatttgatt
    20161 tctgtatgcg gtgagtctta aatttttttc tgaaagagtt ttaaattgct ctttactgac
    20221 taattcaaag gcttgaatgg ctgcactgtt ttctgatcct gctctatgat tattttcttg
    20281 gttcccacct ttaaaaattc caaattcttt ttttaaataa ctctcaggag aatagacaag
    20341 acttgctccc attcctgctc cgattttatg accgctgaat gtaaatccat ccaatagcga
    20401 ataattaatg ttaatctttc cgtatgcctg cattagatct gaaaagattg gcattttgta
    20461 ttcttttgca atagaatgga tatcgctcat tggttgaatt acaccagtct catttgctgc
    20521 ataaatcata aagattggcg cgggatcttc tttcattttc gaagataggt cctccaaatc
    20581 aatgagtccg gtcttatttg tttgaatcag ctcatattga attccataaa aatcgagagg
    20641 tccataaaag gaggaatgct caaaaggaga aatgaatact cttttaaatt gttttagata
    20701 atccactaga gacgcaacta acaaggtatt tgcctctgtt cctgtagaac agaatacaaa
    20761 ttgcttttta tcttttccag ttatttcact aaaataagag cgcgcggact caatgataga
    20821 ttgcctagaa agagaaaagc gagtagcacc tgatggatta tagaaattga ctgaatattc
    20881 agtgtatgcc tccgaaacta tcttagtaaa aggtggatgg gtcgcattgt aatcaaaata
    20941 agaaactcga ctcactctat ttcatctgcc tctttgaaac gatccagttt ctttaatgtt
    21001 tcccgcagag tatttttata atcagtcaaa agatctattt ctattttccc accatttctt
    21061 ccatctacgt tggcaagtaa gttctcgact gactttagag cgattaacgc ttctttgtaa
    21121 ttacctaact taaaattaca tttagcaatc attagatttg attggaaaat gtgactcgca
    21181 atgagtcgca tggatacagg agattcgtct tttaaatctt caaattcttt tgctgctgta
    21241 aattgctctt tggctctttc ataatcaccc atattgaaaa gtaggattcc tcgcttgaat
    21301 cgaaagaatc tttcattttt ttgctgacct ggatctgttg ttccttgcaa gaaaaataaa
    21361 attgtttcgt ataacgcatc tgcttcttta ggtgatgaat ttctgatatt tgtatctagg
    21421 agtagaatat ttaatcctcg gatataatcg gaaagaagag gcaaattggg atcttctcca
    21481 cttccacctt taattccttc tttgatattt tttcgaattt ggctccagat aatagatctc
    21541 ttcataagag gattcaatcc atacatttct tttctttctg caggtcctgc cattccaaaa
    21601 agtctgagga tcggagaaaa gaaagattgg ttttttttag caaactgtct gtcttcttct
    21661 tctagaattt tttccacata tgcatcagat acagttatat aactagtata ggctaaaata
    21721 atttcttctg gtctacaaac agcttttgca acagatctaa ttttgtctgc tgccagaatt
    21781 tctggacctg aataattgag agctcgtttg taataatcca gcgcacctaa aacagaatcc
    21841 acattctttt tccaatactc ttccggattg agcacggtgg gcagatgacc tctgcttttt
    21901 ccaattccat ctgcagaaag ttctctatcg ctagaccatt ttgtatttcg atcgagccaa
    21961 gaagggtatt gtaatacttc atcttttccg gcaatttttt tataataatc acaagcttct
    22021 tccattagtt ctaaatgaga gagtaagata gctggtcttt ttttttcatc ctctagttct
    22081 tctttgattc taggagaaac aatggcatcg tctagatttt tatctaaagc tttgtctcct
    22141 ttttctatga actttcgtgc tactttaggg gagtagccgc tcggtgacat cgaaattgca
    22201 taccagctaa agagggaatc tcggttggag attacaattg gaaaagcaat tgcccatatc
    22261 aaaaacagga gtagtaaata aaccaaattg tcttttaaga ttttaatcaa tggcttcctc
    22321 gttaaatttg ataggcatat taatagaatc gtaaaaaaac ccgattctgt aaattaattt
    22381 attggtttcg gggcgcgaat aattagaact agttgaattg tttgatttag aagaattaaa
    22441 agattaccag cgatgaagac gaggggcacc gattgctcta acaaatagat aagttttgtt
    22501 cttttatctt actccttgtt aaagttttta aggttcaaat attcgccctt taaatttata
    22561 taaccgaagc cattaagtcg aaataggaat ccatcgtata atatcgttca gttatcggtg
    22621 tcatcggtgg tgaaaaataa ttcctttttt gttttatata ccgattgact cattggttga
    22681 ataacaagaa tgtatttctt acagttacag actggtttaa tctagagaat gagtatttct
    22741 ggaggagagg atgaaaaaag ttttattcgt cataatgctg tttttttcga ttggcatttg
    22801 ggcagatgag ggagcaaggt gtaagccact agcaggggag accgagtatt atagtcctgt
    22861 atttcttttg tctatgggaa ttaaaatgca gtcccaagca aatcatgcaa attcgaaaaa
    22921 tccacagcaa gttgtatctt tgcataaaaa ggcaattcaa tactttcacg cttatattca
    22981 gtgtgcaaaa gaaaatgcga tcccagtgaa cagttctacc tattatctaa aagcaaccag
    23041 tcattttgaa attagagaat ggaatgaatg ttctacggat ctagacacat ctctagcttt
    23101 atcaaataat caatacaaag atgcactgat tttaaaatct agacttctca ttaagcaaaa
    23161 taaatttaaa caagcggcag acttactaga gagtgttatt tcattttatt cagaagattc
    23221 cgatctactt tattttttag gttcactcag tgctgaggta gaaaattatc ccaaagcaat
    23281 tttatatttt acttctctgt ataatttgat tcagaaaaaa gaaggagatg ctaaatatag
    23341 aaatttcatt ctaaaatact taggtgatat ttactctaaa aagggagatg gacaaagagc
    23401 cattcattat tacaaacttt atctaaagta taaagaaaat gatccagaag tgttttttac
    23461 tcttgctcag atttatataa cgttaggcga ttttaatgaa tccagaattt atctaaataa
    23521 aattttaaaa acaaatccag actcaaaacc tgtcattcaa ttattggcag aaatgcattt
    23581 catagaaaat aaaatgtttt ctttgagtta ttttgaaaat ctacacaaag agggtaagtt
    23641 taaagaagag gggctattag cttcaatcta taaagttcta gttggaaaac acaaagaagc
    23701 agaagaatac tttgtaacca tgtcttctaa aaatccaggg cggctatcgc tacgaatggc
    23761 tcttattatc atctactcta aactgaaaaa aactccagag cttgcaagtg aattaaaaat
    23821 tacttctgaa attgcctatg caaataaaca atacaatctt gcgattgaac ttgcgatgca
    23881 acttttagat atttcgctga ataatcctga tttaaaaata aagcctgttt tattgtatga
    23941 ttttatcgcg acctgttatg aagacagtgg cgcgcccttt ctgggaattc attatttaag
    24001 aaaggcattg gatacattag aaagtgattc tgaaagaatt aatctaactt tgcatatggc
    24061 gaatatttat agaaacagta aagtaaagag atatgatgaa tccaataaaa tactgcaacc
    24121 tattttgaag agtgaaaacc caaatgctta ttttattcaa ggtatgaact atttttcaat
    24181 ggaacaatac aaagaatcaa ttgaatcttt ttctaatgcg attgcgattg acgcaaaaaa
    24241 tccaaattat tattttttca gagcttctgc ttatgagaaa aacaaacaat taaacgaaac
    24301 aattcaagat ctaaaaaaat cattagagtt agataatact aatgcggcta cctacaattt
    24361 tttaggttat ttgtatgctg aaaacaatat ggaattagtg gatgcgtata actttatcaa
    24421 aaaagcagta gagttagaac cagacaatgg atcctaccaa gacagtctcg gttgggtatt
    24481 gtttaagatg gagaagttaa aagaatcctt acatcatcta aacttggcac tccaaattat
    24541 gacggacaga aaagaagatg atcctgttgt gtatgatcat ttaggagatg tattctttaa
    24601 aatgaacgat atggacaatg caagtgaaaa ttggaaaaaa tcagttgaaa tacaatccag
    24661 tccaatggaa aaagagaaaa ttttacaaaa aataaaaaaa ttaggaagca gtaaatcata
    24721 attagccgga agaaatatga aaaaaactat tagtatctta tttttattta gtttaatctt
    24781 actcggatgt aaaacagagg aagggagcgt aaaagagccc gaatacacag atggaaagca
    24841 aacccaaaat ttaaaataca acgaccctcg agcaaagtcg ttagtggaaa ttgtagaaag
    24901 attggagaaa aattcgaact cttttacggg tgaatttacc atgaagattt tatcgggtga
    24961 caagttaaaa gaatccaata ctgtgaatgg aaagattttc tttgataaac caaccggaaa
    25021 agtaaagatc caactgatgg agcctttctt tggtttgatt atttcacaga ttatatcaga
    25081 taattctagg attcgaatca agtcttccgg acaagaaaag attcacgaac aagcgatggg
    25141 cgatatctat atggtagatc ctaatactag gaaaactatt gtgattcctt atccaattat
    25201 ttattttagc attgcacaaa atttcatcga agagtttaga tcggacaaaa gctatctaag
    25261 tccttccgac aaacgagttc tagtaaaaag aggaacggat gagtataaat atttctttta
    25321 tgataaaggt ttatcttctc tagaattatt tgctcctgat aaaaacattc gcgcaatttg
    25381 tcaaatccct gagaaataca gaacgggaga tcatcctcca gaaagaatct ctactcgtgt
    25441 gacagatgcc acaacggaac tagatacatc tcaagtagac atccattata aaaacgtcaa
    25501 acgaaatgct actatccctc ctaagacgtt tgagttttaa ttcgacgtaa aagattacca
    25561 catgcgaagg cgttggtcgc gaatacttgg agcgaagggt ggactaaaca ccgataatac
    25621 acaccgatcg ctttgcttgc actgctaata ccgataaggg tatgctgggt gttgtttttt
    25681 gtcaatgtta ctggattgtg taagaattat tttaccacga agagcacgaa gggacacgaa
    25741 gtagggtctt gatttatcaa gaccttcttt ataggataac accgatttgc ctggattgaa
    25801 ataaaatgat aaaagaaacc tgtggctcga aacccttgca cggaggcttc tgcggctaat
    25861 agttgtttgt tcctctaaaa tttgattccc attgatattt tgaaaagcca ggtgtttctt
    25921 ggatactgta ggaatgtctt tataaccttt tgagctctta aattttggcg aaaatttccc
    25981 cctctagttc aaaacacctt cttatgaaag actcacaatc ctttttgttt taagcttttc
    26041 acttgtagga tagaaatggg agacaattat ggttaaagtt gggtgtaaat taagcgatag
    26101 acgctatata taaaacctat acaaaaataa atatgaaata aagaaagata gcaaagatgg
    26161 atacttatca tattagccct tatcctgaac ttacatcaaa tatagattcc attcaatatt
    26221 tttggcatat gcttccgtat gctgtgcctg gttttattac ttttttttgt ggcttgattt
    26281 tagccttaat cggttttttt agagtatttt ctcgaaaaga aaaaactggt tactacctct
    26341 ctttcagtgg tatgtgttta gcctttggaa tgcttggatt tgtattagct cttcgagcgg
    26401 ttattcatga tacagaaatc ctattaagat ggaatacagt tttatataca tttattcttt
    26461 tgtatgctcc ttcttctgct caccttatct attatatcct agataaaaaa tataaaattg
    26521 ctttggtagg agctgggatc aattggattg caactctatt tgccttctat ggaattgtaa
    26581 ccaatcaagc gttcactgga aattttttgc agtatagttt tggtatttat cctatagcgt
    26641 ccatatattt gaaggcgtgg ggaattattg gagctgcaac attttttttt ctgggtgttc
    26701 ctacttatgc tcactatctc aaacgaaatt ctttttacga aaaacgattt ttagtaatag
    26761 ggcatatctt aagtattttg ttagtgatat ccaatttacc aagctttatg ggtattcctt
    26821 tgtttccagg aagcacgttt gcttttattc ctatgttgct tttagcatac ggaatttttt
    26881 tgactgattt ttctgatctc agggtaatgc tctttgagaa aaatggattt ttttacgtat
    26941 tgaatttatt cgcaagtcta gtacttcttt taattgccat agcagttatt ttctttttct
    27001 ctcctttgca atatcaatat caatctaaaa gtttattaac ttggcaattg attcctttga
    27061 tttcggtttt attggttgta ggtctgggaa tttttatcgg agggagtaat ccccataagc
    27121 cgctcaatca aatgggagcg ttttctcttt acctgtatgg atttcagctt ttggcgagta
    27181 tttctattgg actatcgccg gatccaatgg ttggacacag aattgagcaa ctttgttttt
    27241 tgctcttttg ctttgccccc tctatacaat cccgatttgc gtttttaagt ttcgaaaaga
    27301 aaattccgaa gttttttatt ttgatagata ttgcatcctt tttacttgct gtactcgctc
    27361 tgagtcctta tctttttgtt gggtattata atttcccatg gggaagattt tccgcatctg
    27421 gacccgtgat tcacggtctt ggaatggttg gctttatcgc catgattttt actttaaatg
    27481 aatggaatca aaagagaaag cgaaaagaaa tcaagccaat cggaaacttt gcagtctttt
    27541 accttgcaac cggtgcaatt atgttgctgt ttaatattcc cgcaacgatg ggaattcccc
    27601 tttatccgct tggtaatcta tccatctttc caacgatgat attagtgtat gcaattgtaa
    27661 gatacggaga atctacaatc aatacagagt cttttcgaat tagttcttac ttagttcctt
    27721 ttgccctcgg aattgttatt tttatactct gtttagtttg ggtaaatatt cctagagaag
    27781 tttcttttga aagtagactc tttcatttgt ttatcagtgg agttcccctt atactattaa
    27841 gttttctttc tacatttatt ttaattcgtc ctattgcagc aagagttgat aagactctcg
    27901 aaagtcttcg gagagagaaa gaaattactg aaaagcaaaa gatggaaatt gaaattataa
    27961 atgattactc taaaaaacta aattcaaaat tagatttaga taaaatttta gatgaaattt
    28021 ttgaatactt tgatgatcga tattcgattg aaggtatcat catccaattt ttagataaag
    28081 aaaagaatga attttattct tataaaacga caactcccga aaatgctgca caggaaatta
    28141 ttgattacag tcaaaatctt cgactgcctt ttgatgaaag actagggatt cttttttcgg
    28201 cgtataaaaa attaagaaca ctgtatgttc cccgcgttgt ttacaatctc caaccaccta
    28261 ttatacagga tatgatcaac gcccttcgtt ttcaaaattt tctattcgtt ccgctaatca
    28321 ttcaggatga agtgataggt agtatgtttt ttacatccta ccgaaagagg atcgatttat
    28381 caaaaactga tatttcaaac atatccattt tttgtaacca gatatccact tccattcaaa
    28441 attcgaaatt atttaaacag gtaaaggaag aaaaggaaaa agccttagaa gcaaagctag
    28501 aagcagaaaa agcaaaattt gaagtagaaa tcttaggaga ttttgccaaa ctagtcaatt
    28561 cagaaaacat actcgatcat atcttttcgt atgctgttat caatttaatt caaaatctaa
    28621 atgcagatat tttctggtta caattagttg ataaaaataa aaatgaactc tatacacgtt
    28681 gtatctccaa ttcgggtaac gttccagaag ttataaacaa attcaaggca atgagaatac
    28741 ctctgagtgc ccaatcaggc tccatttatc tcacatacaa aaggaaaaaa attctctata
    28801 tttctgatat ttcaaaaaca agatcaatgc tttctgagat tgataaatct ttgataaatg
    28861 aattcaatct aaaatcagcc tttcaaatac cgttgctaat taatgatgag gtgataggaa
    28921 tacttcatat taacaagttg ggtggaatga aaaaactaac gcaggatgat attcgttcta
    28981 cggaagcgct ttgtgaacag ttgggaatcg caatcaataa aacagctctc tatgaaaaag
    29041 cagaattaga aaggcgaaag tcagaaaatt tattattaaa cattttacca aaggatgttg
    29101 cttccgaatt aaaagaaaaa ggtgcggcag atccagttca atttgattca gtgagtgtat
    29161 tatttacaga ttttcagggc tttaccacca ttgccgaaag gatgaaacca aacgatttaa
    29221 tcaaagaact agatgcttgt tttattcaat ttgatagaat tacagagcgt tgcaatttag
    29281 aaaaattaaa aacaataggc gacagttata tgtgtgctgg tggaattcct cgatcaaata
    29341 aaacgcacgc aattgattgc gtactagccg ctatgcaaat gcaagacttc atgaaaagaa
    29401 caagggaaga aaaaacagaa caaggtgttc cttattggga aatcagactc ggaatacata
    29461 gcggaccact ggtagccggt gttataggcg agaaaaaatt tgcttatgat gtctgggggg
    29521 atacggtgaa tacagcaagt aggatggaat catctggaca tcccggaaaa ataaatatca
    29581 gtggatcaac gtatgaactc attcgagatt atttcgaatg tgaacatcgg ggtcgcgtaa
    29641 aagcaaaaaa caaaggagag attgatatgt attttgtatt acgcttgaag ccggaatttt
    29701 ctgatgatga agaaggaaaa ataccgaatc aaaagtttaa agaaatttat aatcaaaaaa
    29761 atagatttta cccgatctag ttcaaaatat ctttttatca aagactccta attctttttt
    29821 gctttatggt atttactcgt aagatagaaa cgggagacga tgatggaaag agttgaagac
    29881 aaaataggcg atagccgcta tagagaaaaa atatagtaat tgatcttaga caaataagaa
    29941 gattatgaag tttaaatcga aataggaaat attagaagaa tctaatgaat aaaattaata
    30001 gcctctggta gaatctcagt taatgcaata aatttaagga ttctatccaa agatatcact
    30061 tctattgagt ttcgtccaca tctagagtct gtatgacttc tagtattcga gcctccaaca
    30121 aatctccgat caaagatttc ggcgagaggt ttcgggggtg gtagttttaa attaattaca
    30181 ttagctccta acaccaagca agattctaaa cgataaaaaa attatagatt tactttcgat
    30241 gcattgattt ttaattcaca aaaataaaaa tcattttcta atttttcaaa aaaaattccg
    30301 cctatgagct tgactctttt ttgtatcgtc ttatgaccat tacccgcttt ccatggaata
    30361 ttattaaatt cagagtgagc atgcatttgg aatagaatcg agtcttccga attcttaact
    30421 aaccatttgg ttttatctcg tccatacttt aaatcattgt taattatctc ctgcaaaata
    30481 ctgaatagat tgtgataata atcctttggt attgcatgaa tggaaacatt tgaatctaca
    30541 tctaattgaa tttttctttc tgcagtagaa taccttctaa ctaagaaatt tttaaatcct
    30601 ttgttaaagt ttttttcaat gtatgaaata tcttcaaatg cgagaagatg atttctaagt
    30661 ccttttagaa tttcatcaat ggtttttaca atcctagcct ttagattttt actgatcgtt
    30721 ttatctggct caattgactt taactgtaag gaaaggatta aaagttttcc acctaagtta
    30781 tcatgaatat ctacaaataa ctcttgtctt tctttctgaa ttgcgatttc tttttcgagc
    30841 gcatttactt tttgtatttc atttaagttt tgtttgtaaa taatttctaa ttcatccgtt
    30901 ctaagtttta ctttatgctc taattctctt tggagtgtgg ttaactcatc gttaatcgca
    30961 ctcaatgtat ttttttgttc tctcaattca atagattgag tctctgaaat ttgaaatgct
    31021 ttggaaaatt tagttgagag tgaaaaagat tgaaagaaca aaaaaccaaa tagagtaaag
    31081 ggtgttacgt aatgccagaa tacaatatgt tcattgtgca aaatatcaat cacagcacct
    31141 atggctaata tgattattcc gataaaggag gaaagagctc cgtctctttt cttaaagatt
    31201 gccgaaaaat ttacatagag tagatagggt atggaaagaa aaacatgaat ctcaaaaaaa
    31261 ataagaagat gagtatattc ataatgtggt aacaataaaa cagtaagaca aagaaatgaa
    31321 aagaatatcc aaattatttt caaataacgt ttattgattt cttcttcgaa taaataattc
    31381 ataaaggaaa cgaagagggg aaaggctatg tatccgcaaa tatattccaa tctgtaggta
    31441 aaatcaaaac ttaaaaatgg aaaggttgac attagtattc tttcttctgt cacaattgtg
    31501 cgaatcgcaa aattcaaaca aaaaaaacca aagaaaaagg tgcttttgtc tctcgttctg
    31561 gtataaaata aaccaaaatg gtagatgctc attataaaaa gtccaccaaa gaccatcatt
    31621 tctaaagata tagttacaaa tttttgctta taaatatttt caactgtgcc aagaacaaaa
    31681 cttccccaaa ctccgcctgt tctatccata aaatttgaaa tttgtgcagt aatatgaaat
    31741 tcatgtttat tttctaaatt gagtaaatga ttctgcggca taagataggc tgtcatttcc
    31801 tcttttgtct ttccaatttt tccagcttca aaaattaact tatcatcaat ccaaacttta
    31861 tttgctgttc ccatcactgg aattcgaagt gccatatcgt ttgtgcctgc taaaagtttt
    31921 acatgcaaat ggaaagtggc ataaccatct ccggctaacg gtcttttact gataagtttg
    31981 cctttccagt ttcctggtat ttgaattaaa ttttcattct ctttgagact tttaagaggt
    32041 acactttcca tgatatcatg aaaggttaaa aattgattcc agtaaaaatc ccattctcca
    32101 tctaacgcca catttccatc gcttgcaaaa tcccaattga ttaaatcgat agttcccttg
    32161 atagccttta cttcgtgatg tggttctttt ttatcacaag aaaggattga aaatagggta
    32221 agtagaacta atattaagtg cttcagagtt tctctccttt ctagtaaatt atctattcaa
    32281 tcgaggaaag cttttcattg gatcagattt taccccgttt agttcaaaac accttattat
    32341 aaaagaatcg caatcctttt ttattgtaag catttcactt gtaagataga aatgggatag
    32401 gatgatggaa aaagttcgat gcaaattagg cgttatccgc tagaaatata ttctaagtgg
    32461 aatcattatt actttagcgg attagttgct aggcaaatag gtccggattc ttctcaatta
    32521 tgagctaatt ttaccttcac aaataaaacg aatcaatgta ttgactgctt tgataaaatt
    32581 atgcatcttg gcgaaaggat cgttatctat atctcggcaa ttaattttat ccctattatt
    32641 atttatggct gatgataaat gatatgcctg ttcaacttac atctcgcaag cttttcggct
    32701 ctactccata cgataggaaa gaattctata tatttattct ctagtcataa aaatattctg
    32761 gcgaatatcg tatttgcttt tctctgtttc ttgtagtaaa gaatgaaata tgcaatgaaa
    32821 attattagaa acaacacgca aaaatagtaa aacaaaagta gaacgaaaaa aatatgagtc
    32881 tgtaaaatca ttaaaattac ataaatattc aatggagaaa taaacaaatg aatcaaatta
    32941 gaattctaat tgcaatcata accgcattgt ctttaggaaa ttgcaaagag gaaaaaaaga
    33001 taatggacca ttacttttag gtgcagcagc tttagcgaat caaataagaa ataattcagg
    33061 tgccggtagt tctggcgcta acgccaatac aacagtagtc actcccacta ttacgtcacc
    33121 agcagcaggc caatatagta cgtctcaaac tattacaata actacaacca catctgatgc
    33181 tactatttat tatacggtag acggcacaga tcctactact tcttcgaatg tatacacaca
    33241 ggctatcagc atttggagtt tagcaggaca atctagaact ataaaggcta ttggagtaaa
    33301 atctggtttt agtaataata gtattgctac tcaaacagtg ggtgttttca actatatccc
    33361 tcacaagact gggcaaacac tctgttggga tgcagtagga aatccaacta cttgcacagg
    33421 aactaagcat gatggagatt tgcaaaatgg cattacaaga agctatacag gtcctactca
    33481 gaattctact tatacatccg attacacatc cacagacaat gtaacaggct tagtttggaa
    33541 aacttgttca cagggtttaa gtggtgcgac ctgtgcgtct ggaactatag ctcctatgaa
    33601 ttggcaggat gctcaaacag gttctaatgg ttgtagcagt ctaaatgctc ttaacagtgg
    33661 aaatggatat gcaggcaaaa aagactggag acttcctacc cgtcaggaag tagaaacatt
    33721 gacagatagg ggaagaaata atcctgcaat agacccgact ccttttcctg ctacgtcggg
    33781 tagttattgg acttctacag cttacggact gaatacggca aatgcctgga cggttcaata
    33841 taatgctgga agcatgaaca atgttgttaa gaccgataac acagtcaacg tacgttgtgt
    33901 gtcaggtctt tccagaggat atattggaag cttcacagat aacggtaatg gaaccattaa
    33961 ggacaatgta accggactta tatggcagaa atgcagtatg ggtcaaaaca atgatgctac
    34021 ttgttcagga accgcaactt ctagatcctg ggcaaatgct ttagcagatt gcaatgggct
    34081 cagtcttgca tctagaacct ggcgtttacc caacgttaat gaaatcaatt cgatagtaga
    34141 tacaactaaa acgagcaatc caaccattga cgctacattt tttccatcaa ctagcagcat
    34201 ttattggagt tctagtaccg tcgcgattgc tctctccagt gcgtggatac tgcctttcgg
    34261 aagcggcggc aatgtcacag accaaaataa gaataatacc tacaatgtgc gttgtgtatc
    34321 tggaccttag ttcagatact tgcgattcaa gtttcagcac gtttttggaa agcaaagaag
    34381 tgctgaaatt cgtgggaatg aaatttgatt ttttgtatct cacccaattc tttttgaata
    34441 ttttccattt cataaattat gaaaaaattc tccccctcta tttcaaagca acttctttct
    34501 gaatgtattt cctgaaaata cgttatttcc agaacatagc ccagaaatag ccaacaagga
    34561 cgagccatga aatcacttgt cctaaaagaa aagcagtgcc tgctcctaat actccgaatt
    34621 cggggatgag cgcagtatct aaaatatatc caaagacaag tcttagaagt gcaaggattg
    34681 ctagtattct aggctttccc atggcgaata acgctatccc gagaggtgca aatacaagtt
    34741 gtaacatata attaggatat agaattttaa aaacaggaat tgcttcctcg tatttatgat
    34801 tgaataaaag atttaaaatt ggctctgcga gtagataccc gggggagagt aaaattgcaa
    34861 tagcaaatcc agcaaagaca gattttaaaa gaaaattttt aaactcatct gtatgcacaa
    34921 gagcagaaag tcttggatag atgagtgaat tgagtgtgga tagaatgatt acaaatccac
    34981 taaaaagttg aagtgctgtt ccatacactg ctacttgctc tgaggaatgg tatttattga
    35041 ttagaaaaat ttctaatcgg tctgagataa ttgcaaagat agaagccaca aatgcccaag
    35101 tattaaaaag agaaagttcc tttgttacat gcgttatttc ttttagcttc cctccccaat
    35161 agattttatt tcgattgaat ataaaaaaga aaaggattaa tataaaaatt ggacttaaac
    35221 taaatatagc aagaatgtct aggtaggtaa gaggtagttc tgaatacttg tgagtgaggt
    35281 ataataaaat tagccgcatt acattggcta aagggttcca aaaagcaaga gccttataag
    35341 cttgaaacga aacaaagata ctttcaaaaa aagatacaaa ggaaatcgtc gttcccccaa
    35401 ttaacagaat aaatatgata agagggtctt ccgcgtaaaa ggtataagct ccaaatccga
    35461 gtgcgagaag gagtaaaaaa gaaatgatct taatcagtaa agatgaggat aaaatcactc
    35521 cgattttttc tttattgtct gatacggaag aaataaattt tacaatcgca gatggcagac
    35581 caaactcggc aacggcaagg atcacaggta gatatcccaa atagtattga aagattccat
    35641 tttctgcttt gttcaaaata tttactgcaa atatcataaa aagtaaattg cagatcgagg
    35701 aaaatacttt cgataaactt acataaatag aggatttaaa gaatttagac tttaaaaaac
    35761 ttttaactag attcagaatt ttacgaacta aagtcattct ttttactaac tcacctcaga
    35821 ttagacgata tacgattgat atatttgaaa tcattttgat ctaaatcgat accaaaagag
    35881 tggagccagt aaaacaatat agagattggt cagtaaatac cgtaagtatc taaataaact
    35941 ttcttcaata tgaaatgtct ttcctaggat tccaagacct agataaaaaa ccaatattcc
    36001 cggaagtaaa aaaccagctc gaaccaagaa atctttgaaa ctacttacca cttgccattc
    36061 agatgcttcc gaatataaac cttttaaaag aatcagtcca atcgaaaaac ctgcaatgga
    36121 accacttcca attaaaacct gttctagaga agagatttgt ggatgtgtga ttccttttgg
    36181 aagcagagtg ctcgaaagtg tcagagcaag aataaaaagg gaaatggatc taacgattct
    36241 attctttgaa tcttcattaa attctatcct tggaaagtct ggaatcttat aaaataaaaa
    36301 ctcaatgagg ataaggctga ttagccccat gctaaaacca ccaatcacat ctccaggata
    36361 atgcacgcct gcatacattc tactaaaggg tgtaaataga attacaaaaa gcgcaaaagc
    36421 tctaaagaac ttgtttttga attcaagaaa taagatcccc caaaccaaaa tcgaaacatg
    36481 agaatgaccg gaaggaaatc caaatgaggt ttctttgatc aaatcacttt gcacagcaag
    36541 aatggaatcg ggtagatcaa tcggtcttgc actttcgaac atatactttg cgagtccgtt
    36601 aaaaaaagca gtggagagaa gtgcgactcc tagtttgatt ccaaaatttc tattatagca
    36661 taaatataca aatggcaaaa gaaatacaaa aaaatttgca cctcccataa aatgacaaag
    36721 agtagagagc cacctgagta tatctccaat ggttccagtc ggatgaggaa ttgcttctaa
    36781 aaataaattt ccgaataagt atccgttttc aaataataca tggtgctcgt tcatactagt
    36841 cctctttgaa ttttctaatt ttatttatat ttttacttat taaaatggat tcacctgtaa
    36901 tcttcccctc aactttatga atccctttta aaatagaatt ttctgcaatt cctgttgcta
    36961 caaatccaaa aacatttgcg ggaacta
//
LOCUS       DAOQOS010000048        36763 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_467_length_36763_cov_6.496676, whole genome shotgun sequence.
ACCESSION   DAOQOS010000048 DAOQOS010000000
VERSION     DAOQOS010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 36763)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 36763)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36763
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_467_length_36763_cov_6.496676"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1267
                     /locus_tag="PLF66_12115"
     CDS             <1..1267
                     /locus_tag="PLF66_12115"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: protein motif:HMM:NF014895.2"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     pyruvate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HMZ64835.1"
                     /translation="IFFLLYNPYFRRLDKVALEIDLLHRKMRVGEVVVEEGEVISIDG
                     NSGEVFIGKIDTVVPDIKDPYLIKLLHWADEIRKLEVWANADYPVDAQRARDNGAQGI
                     GLCRTEHMFFQTERLPTVQKMILAKEEATRQEALNALLPMQRDDFIGLFRVMNGLPVV
                     IRLIDPPLHEFLPTISSLLLDLSDLKIRLLRESNNLGQVDAILATINEKKTLLERVEE
                     MHEQNPMLGLRGVRLGIHLSALTRMQVRAIIEAACTVTKEGIHVIPKIMIPLTSHVNE
                     LKQQKEILEEEAKQVLSERGMKISYKFGTMIETPRAALTADELAVEAQFFSFGTNDLT
                     QTTYGISRDDAEKGFLIEYLEQGILKDNPFATIDQRGVGKIMQIGVELGRKTRPDLEI
                     GICGEHGGEPASVHFCHTIGLNYVSCSPF"
     gene            complement(1510..2283)
                     /locus_tag="PLF66_12120"
     CDS             complement(1510..2283)
                     /locus_tag="PLF66_12120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HMZ64836.1"
                     /translation="MKIKIIYKFCFFLYLFHFSVLALPISPDGIGAIVEEKLRTEGYY
                     FPNREIPLYAITDEGKFVHDFEYSIVYAKENFEDIQKGELCIQESGQKYLKIKFSDFI
                     SLNYEGRYFIVYEQKKGFVRILRDTVRNMTDKNPKDLSLGFFVKLSDLKKANFYSETW
                     IGIIQKKETLYNSVKIGLNLRKSPDSKSEKLTKILKTNYEIIPSRETKGQWLKVKVLL
                     HRGEFCEAGDDVIEKEWEGWIKAIDDSGKPNVWFFASGC"
     gene            complement(2424..3305)
                     /locus_tag="PLF66_12125"
     CDS             complement(2424..3305)
                     /locus_tag="PLF66_12125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64837.1"
                     /translation="MAPLILKYIRISFLFILGIFYSSCASMSVYNLVYPYSIKPDPEI
                     VKEVSMDESKRLRLRLKSDIFFESLRCSEENLDDHFRQIHGKDIFDCNEKTDKFIKLN
                     IDDSIPKTWLPILDKGNSLLDLSTIDSASYLLYPNVELYSFDLESAYYNENRNRIILI
                     DKSGQCFLFGRHIFSDQGAFYSAEDPWKKLYEKEECKKYLASSMLKEIPIRKMKMPII
                     IIEYNGFTRSSFKLASSIRTIDRNQYIQIKSFSKDPNYIYSPLFLMSVPFDIVTSPIQ
                     LIIFGILYYIAPGTLHN"
     gene            3655..3849
                     /locus_tag="PLF66_12130"
     CDS             3655..3849
                     /locus_tag="PLF66_12130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64838.1"
                     /translation="MSIQPIDPLVLFTDRIFEPFNNSSQEYGDERLYDFLLNSQKLPM
                     NEVFTKLIADVDPFFGEKIR"
     gene            complement(3888..6053)
                     /locus_tag="PLF66_12135"
     CDS             complement(3888..6053)
                     /locus_tag="PLF66_12135"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HMZ64839.1"
                     /translation="MHLKQGKFRGLTSLLPSILFLGLFSFLFYCKGSSNSKQNPLLQE
                     GVLNLSDENISSQTFMQNGFLKLDGAWQFYWKEFKSPDEIKNNTQPKSFIKVPSSWND
                     FIFEGKKVGGYGHATYAANLRLPSEWIGKILTLKIPHMGTAYRLYVNNELISENGKVG
                     ETKDEMKPQYINRTKSFVVTDQSTHIIIHVSNFHHRKGGMRFDSVYLGWEESIQSVQT
                     SNSLYIFFLMGSLAIMAIYHFGLFFLRKEDIAALVFGAFCVLISIRLCFVGDVWITSV
                     IPNLDWAWMYRFEYMSLYLSAPVFSIFTYYVFREEFNRKVLFVSVGFYLLLTTVVLFT
                     QPYFFSLSLILFQVVSFINTLYVAFVIGLAIYKKREGSIAAMIGFLFFTLAIINDLLY
                     NNEIILTGNGNLIPFGVFLFIFSQSFILSQIFSNAFKSVKILSNNLSKTNEAYSRFVP
                     VQFLNYLNKNSIVDIQLGDQVQKDMAILFSDIRSFTELSEKLTPKENFNFLNSYLQRM
                     SPVIQKNNGFIDKYIGDAIMALFPGEIEDAIHAAIDMQKEIRVYNQQRLKIGYDPIKI
                     GCGIHSGNLMLGIIGADARMEGTVIADSVNVASRIEGLTKVYDASILVSDIILKKINN
                     PSDYNYRFIDRVKVKGKSDYTVIYEIYDGQPNFMIELKTQTKKFFEDAITFYYDKKFR
                     ESIIAFEKVIEINPNDTATLIYLKRAKYYLENEITEFLE"
     gene            complement(6801..7253)
                     /locus_tag="PLF66_12140"
     CDS             complement(6801..7253)
                     /locus_tag="PLF66_12140"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HMZ64840.1"
                     /translation="MIKRLFFDTSVLIPAFAEAHPNHKQCFSFLEKISNQKIEVVTSA
                     HALLEFYSVFSKLPLKTRISPLEIKEIIEKNIYPNFKVIALEEKDYKEIILQITSLGL
                     SGGIVYDLFHVVAARKVKSNAIITYNEKDFQKINFAKIPIFLPQDFKN"
     gene            complement(7250..7501)
                     /locus_tag="PLF66_12145"
     CDS             complement(7250..7501)
                     /locus_tag="PLF66_12145"
                     /inference="COORDINATES: protein motif:HMM:TIGR01439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HMZ64841.1"
                     /translation="MTVVIDKVGRLVVPKEIRDQLGIGPNSELEILIDGLEIRVRKIE
                     EKSTLKKKGKFTVVSTDWVGETDPVKLIQKARKERDSQL"
     gene            complement(7888..8982)
                     /locus_tag="PLF66_12150"
     CDS             complement(7888..8982)
                     /locus_tag="PLF66_12150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64842.1"
                     /translation="MQEKAKQRNRFYPFFLSIFIFVQCVTTAKIPVVESAPPNFYETM
                     KGARSLGIFVDDIPNSSEDDWKKTIHSGIESALNDFKYFKIVDIHNRSQRLKEMSFAQ
                     KMGDEIRSLGSELSVQALLYVQIPEPPQYDCKLSSSSKKETSCVLRDKDGKCLKNISR
                     NIITYEKTLRYTILANAKLIHISSGQMLVASNSEPAILTNRGKSMNISCPSRSEAHLR
                     AIQIASNNIANTISPRVRPFSIPLFTSAVGVKDEDRIKEVESYLKRGNSWVNSDNPNF
                     EFAKKDWELALSKSKNTSANAFWNLAIFYWQNGEIPKAEEFFQYSIENGGADFFDSTW
                     ENFKLIFRNKRRDVINKFYEEKKRYELENS"
     gene            9314..9652
                     /locus_tag="PLF66_12155"
     CDS             9314..9652
                     /locus_tag="PLF66_12155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010568732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HMZ64843.1"
                     /translation="MAQNKKTEFKENIQNLSEFAKAISHPARISILKTIAQKQECICG
                     EIVEVLPLAQSTVSQHLKDLKEVGFIKGEVEGTKSCYCINWKKVEEFEKEIEKLFQDL
                     KKHKNEDNCC"
     gene            9818..10306
                     /locus_tag="PLF66_12160"
     CDS             9818..10306
                     /locus_tag="PLF66_12160"
                     /inference="COORDINATES: protein motif:HMM:NF041414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsI/CadI family heavy metal resistance
                     metalloenzyme"
                     /protein_id="HMZ64844.1"
                     /translation="MNNVLEKSFPQVGILKPHIAIVVKNVEESIAFYTKMFGKKPSKV
                     RSGYAKFDLEFPSLNLTLNESGKNVPHGALSHLGIQVSSTEDVLKVKKYWEELGLTAR
                     DELGVSCCYAIQDKTWVKDPDGNEWEVFVVLEDNLPEFKFIGGTANACCSTSSSSGQS
                     CC"
     gene            10308..10553
                     /locus_tag="PLF66_12165"
     CDS             10308..10553
                     /locus_tag="PLF66_12165"
                     /inference="COORDINATES: protein motif:HMM:NF024590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HMZ64845.1"
                     /translation="MKIKILGTGCQKCVALEYQTQTAVNQLQLDATIEKVTDMEEIMK
                     YDILSTPALVIDEQVIFTGKVLKTEEIKTLLLQYNSK"
     gene            10550..11518
                     /locus_tag="PLF66_12170"
     CDS             10550..11518
                     /locus_tag="PLF66_12170"
                     /inference="COORDINATES: protein motif:HMM:NF015715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HMZ64846.1"
                     /translation="MIERLIHIILYSWFGLDPSAQLTIAIDFFFYDSIKILILLYSVS
                     MLMNFVNTYFPIEKIRSFLTNKNLFGFEYFLASLFGAVTPFCSCSSVPLFIGFVKGGI
                     PLGVTFAFLITSPLVNEIAVAMFLGIFGFKVTAIYVVSGILIGMTAGFILGNLQQEKF
                     LTEWVKEILNKSVARQTNYILENKSFRERILEASKESFGILKGIVLYVFIGIAFGAAI
                     HGYVPNDFFIQYITKENLFAIPVAVLVGIPLYSNAAGIIPIIQVLVAKGIPLGTALAF
                     MMAVVGLSLPEAMLLKKVMQIRLLLTFFFVVGVSIILLGYFYNLVL"
     gene            complement(12255..12998)
                     /locus_tag="PLF66_12175"
     CDS             complement(12255..12998)
                     /locus_tag="PLF66_12175"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HMZ64847.1"
                     /translation="MIKKIILFLILAFVLGLCKKKDNDLEKEQQLSSTPAKSIEVQKN
                     FKSSKSMQGECVGTSLIVEAKAGLRLREEPSLSAKVITTILYKNLVDFIEDSKVPVES
                     EGKTGTFFKVSYESNIGYAFSEFLTYSKKDETICGFEVKINEVNSETGNYFIGLTDSK
                     NGYECNAMYATLDCFYLVFNSKNHLILDTRDLNEKPEPWKSWLNKYEIDIDAGFADDG
                     CTFTRRYAFNIYNHVRRTIEEKKECSPVR"
     gene            complement(13053..13214)
                     /locus_tag="PLF66_12180"
                     /pseudo
     CDS             complement(13053..13214)
                     /locus_tag="PLF66_12180"
                     /inference="COORDINATES: protein motif:HMM:NF012895.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
     gene            13401..13691
                     /locus_tag="PLF66_12185"
     CDS             13401..13691
                     /locus_tag="PLF66_12185"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HMZ64848.1"
                     /translation="MNLLAYVRSQRKELIAIAKKHGAYDLRVFGSVARGEESDKSDID
                     ILVSFEKGRSLFDEVRLFDELTNHLNHKIDLVGEDSLSPLLKSKILKEAILI"
     gene            13688..14011
                     /locus_tag="PLF66_12190"
     CDS             13688..14011
                     /locus_tag="PLF66_12190"
                     /inference="COORDINATES: protein motif:HMM:NF014043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HMZ64849.1"
                     /translation="MKDDLIYVRHILNEMSDIIEFSKGGENWFFQDKMTQKAIIRSLE
                     VIGEAVKNISADFRESNPQVPWKDMAGMRDRLIHGYFDVSLERVWNVVENIIPSLKKQ
                     MENLL"
     gene            complement(14555..16051)
                     /locus_tag="PLF66_12195"
     CDS             complement(14555..16051)
                     /locus_tag="PLF66_12195"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HMZ64850.1"
                     /translation="MKIHKLYLYLGAIFFVLFFGSCVDKSKPNIQSGEVAKKEEIPSK
                     DYLSIPWVNFQLNQLKSDSTKYTLQRQAKELDEQGFALYKKKKDKEAVTLYEKSIDSY
                     PLGETYYNYGNSLSNIDRLEDSIKAYKIARYLNAPKPEFVLYNIACSYSRLNQVEMSY
                     KYLTKAVDNGYKAFDYIKNDSDMANTRKDPNWNNRFKEIISPSRKKSKEEIVEKEDCE
                     FLGKVWSENSCKEKKITISKTEKENCEILGKVWSESSCKEKEITEVKIEKENFTNSIG
                     MEFVLIPAGEFMMGCSEEENKCFDSEKPAHKVRITKPFYMGKFEVTQGQWKAVMGTNP
                     SNYKDCGDNCPVEQVSWNDVQEFIKKLNSKEGKKYRLPTEAEWEYVARAGTTTKYYSN
                     DLDSIAWYDKNSGRKTHPVGQKKPNAFGLYDMSGNVSEWCEDWYHGGYYGESPSNDPA
                     GPSSSSNRVLRGGSWYYSVDLMRSSYRIKYSPDLRSYGIGFRLLFSAD"
     gene            complement(16364..17884)
                     /locus_tag="PLF66_12200"
     CDS             complement(16364..17884)
                     /locus_tag="PLF66_12200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64851.1"
                     /translation="MNNFFYTLVLFLFLLDFTNCKKSNVEKFDSPLILNFVYPVEFDL
                     VDSVKLDAKKIGKIRFNDIIQVSEREIFQIEKDNVKSEKFFLKVNCSEKINCIWNNVY
                     IDLGKYTLINNKIKSDHYLIGELLTKEEFDKEVSIRNWILDPKKEFPSEINIKKVEDY
                     LSFIENFQDRIITENEIKLVLHALKFPEKVKDINRLRILANKYPIIQNVFKKFFENPE
                     EKDSWNKTILNKLNIEGVKPMTAIDIITDTSKFNELFSNSWSTLSNKFNSQKKVFYFQ
                     EYYLKAVLYGSNEFEVIKSSGNLITKEIQLSIEILDKVNLKINDTIFELQEIESIPES
                     NGITFKLKTNEEEILLKSTNRIPEYFIKETIIGKKTFVSTLPSIETIFEQNNYAKALL
                     LVFIKEADTYSNMEFTKQKKFSVIWQILLNHPEKKRFKYGSKNITVLDNHYGCGDIGC
                     DSKDITCFSESHGKLHLSYTSNEIYNDNPRVHLTFGDQSTEDAVICNRYINYNSEK"
     gene            complement(18030..18158)
                     /locus_tag="PLF66_12205"
     CDS             complement(18030..18158)
                     /locus_tag="PLF66_12205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64852.1"
                     /translation="MSNPIPACPTLCIPKAANKHLRSTKSFGNLNGRRYWKKLNAI"
     gene            18203..19282
                     /locus_tag="PLF66_12210"
     CDS             18203..19282
                     /locus_tag="PLF66_12210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HMZ64853.1"
                     /translation="MIKKMILFLILTFVLGFCKKKSSEPVKVEEPTKNYFSTQWVNFW
                     VAKLEYAPEQKARLAEAKKLNSKAFALYKSKKDKEAVELYEKSIDSYPLGETYYNYGN
                     SLSNIDRLEDSILAYKIALHLNAPRPELVLYNTACSHSRLNQVEEAYSYLAKAIDRGY
                     NAISYIEKDPDMANLRKDSRWESKIQELVNSKQYNNEDLAGQLKISYRPRGGDEFILC
                     SSGVAITPIDPFSGIGGACGDRSAFENTQYMYKIGRWKLTNGDLEITFEKECSPDIIQ
                     SSRGDASTMGCQGLAGTPKFKKCSDSISSGNSDVTFSKDKVSTILANDSKDMDTVYEL
                     NKNYSEPKQCDPNFIPKTLDDLKLE"
     gene            complement(19328..19570)
                     /locus_tag="PLF66_12215"
     CDS             complement(19328..19570)
                     /locus_tag="PLF66_12215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64854.1"
                     /translation="MDNIKIDPMWYVSIVGYIIINFTIKYFLKKYKEERAGANPQTVN
                     PPQAGGDKKPTQGGGDKKPAQASGGDKKGGGPPPKK"
     gene            complement(19589..20635)
                     /locus_tag="PLF66_12220"
     CDS             complement(19589..20635)
                     /locus_tag="PLF66_12220"
                     /inference="COORDINATES: protein motif:HMM:NF024827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HMZ64855.1"
                     /translation="MKSVLIVIILFFSLYCNEMKPPLFPKGVSSVETGWEGKRSLSSK
                     ECQSCHNQTHLEWKDGMHAKAWTDPLFQNAFKIERQEWCVNCHAPLSEQKKEFLDNSN
                     SDKSLLAEGINCASCHVREGKVLGFQNFKNDFHEVIKFDSLSTSEFCENCHQFNFPRF
                     EKNKIHYSNEPMQNTYSEWKNSNSSRTCQDCHYDGHRLLGNHNPERLEKDFSEIEYEF
                     FEKGLLHISLRLKNNRAHILPTGDLFHSIQLEIATDPEYKHFHYRKKWARIYGIGNIG
                     GNTFWNRKLIRNTGLTPNEELISLTIDAPNSNKPLYARLVYYFHDEELGGRNDLPSKE
                     KMYVIREKILRFSK"
     gene            20820..21161
                     /locus_tag="PLF66_12225"
     CDS             20820..21161
                     /locus_tag="PLF66_12225"
                     /inference="COORDINATES: protein motif:HMM:NF015735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HMZ64856.1"
                     /translation="MDKFIFIFRGGDTHISTANSQAAMDMIQTWNDWMQGLAEKGILA
                     GGDALQVSGKHVKGPKKVVSDGLYAKGNEMVGGYLIVNANDMNDAVEISKGCPILIED
                     GSVEVRPIQKR"
     gene            21345..21917
                     /locus_tag="PLF66_12230"
     CDS             21345..21917
                     /locus_tag="PLF66_12230"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HMZ64857.1"
                     /translation="MKTKSGEKEKLREKIIDQSIKVIKKKGISGASVDTIMESLGLTS
                     GALYSHFSGKDDLLALSIEKQINSLTDFVSTLIENDRKEGLKNFIKYYLSEKHVRDLE
                     NGCIFAALISEVAQGPQRIKTVFHAGLDKFYSALEKGLPNDYKDKSRTVQFIFSTMVG
                     SLSIARTMKSGIERDDFLNNVLASVLQLLP"
     gene            22334..22582
                     /locus_tag="PLF66_12235"
     CDS             22334..22582
                     /locus_tag="PLF66_12235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004477020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HMZ64858.1"
                     /translation="MISLRLPQELERKLSEVATMENKSKSEVIKESLLYYINHLSQKP
                     SAYDLGKKYFGQYKSGKQDKSVNHQKYIKDAILKKQKN"
     gene            22583..23005
                     /locus_tag="PLF66_12240"
     CDS             22583..23005
                     /locus_tag="PLF66_12240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016761799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HMZ64859.1"
                     /translation="MIKSLIDTGPIVAFFDESDSYCQDIRNFLKDFKGHFYTNLAITT
                     EVCYLLSDSKKTRNSFLQWIKDGAITILAQENKDFILIQYFMEKYSDRPMDFADASLI
                     VLSESHGIKDILTLDSDFLFYKNKKGKSLNILNKDMIR"
     gene            23214..24278
                     /locus_tag="PLF66_12245"
     CDS             23214..24278
                     /locus_tag="PLF66_12245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010572797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64860.1"
                     /translation="MFNKIKKFTFIFLVLTFPVFAQDSKKEIASDEKETTKKKEEDKK
                     DEPKGPKWFSLNPGVTLESVTVDITGRGHNATMTQGGPGRSTWMFDVKSRNIQFTENI
                     GVYLLLHNSEFLLNNQFLPKVAQADAPSSSESSGSSSSGSSPSSDGRAKQNIGTRIRG
                     NYTMAIPVLFYGNDKIGRIGIGAGQANVSMNGSADFQNPLNTYAFLGLDSSNKVNFLD
                     TVSKYQFITGNINPVSGDPVATYLLSNLSQGNNLELLGLYYASQGLLRNPDVISLLPY
                     IRNPRLYSPIEAYALASLAVSNVNFARQNVFSFMAYYETPKFGYFRFRLAFGGPVFKD
                     NGYKFEFTTFHLALFVPIEF"
     gene            complement(24400..24654)
                     /locus_tag="PLF66_12250"
     CDS             complement(24400..24654)
                     /locus_tag="PLF66_12250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64861.1"
                     /translation="MKRILLDYQYPIYETISISLTLDYDRLYVYYENYLPPAFFRFTN
                     ASGVSKPEIAFDEMLSSKNIYRDPLLNKIKSLRLGAVVVF"
     gene            complement(25138..25395)
                     /locus_tag="PLF66_12255"
     CDS             complement(25138..25395)
                     /locus_tag="PLF66_12255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64862.1"
                     /translation="MKYSIKQIIDEQKSFHSYRYLILRNDKPIALFTHNHRGECESLV
                     TLSDGKEHHLPFGQCSEFLIGGGSTPLELSENALVFIQSLI"
     gene            25539..26570
                     /locus_tag="PLF66_12260"
     CDS             25539..26570
                     /locus_tag="PLF66_12260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64863.1"
                     /translation="MRFAKLIILVIIFFPVLFSIKAETRVELKAGISLSDFQPEFQKR
                     YYLDDLIAGGSDGLGFGAINRKYSQSYFAALLNPFDFKFFLTKSDYVFLFGAEYLGLN
                     GMTTSRMQSNFNYSPTGFFMGEGSIDSKARNANTRLSAGRKFDSIIISGLIMKRDFQS
                     EYEKIVILYAPGFLGRVNAESNYYTSNLFAGFSVTKLFEKGKITTEVSFRPKNQGGIS
                     SAGKIEQLGVIRFPVDIPTGNLSSELLVTNYLYGDKKPNIFGVRILMDYQYPVYENFS
                     ISFTIDYDRLYVYYNSYQPPAFFRFANASGISKPEIAYAEILSSYIIYRDPILNEIKS
                     LRLGALVFF"
     gene            complement(26567..26977)
                     /locus_tag="PLF66_12265"
     CDS             complement(26567..26977)
                     /locus_tag="PLF66_12265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64864.1"
                     /translation="MNKKIILRIAAGLVLFTCLGHTIGIFMPLPPEEVELIKIEDAMK
                     TALVPMPIGKKQSFFDLLLGSNIGLSVYLLVSGISLILISSEEKLQSSNKQILKLESL
                     GLLALSVISIFYFFPIPAICTGIAGILGLYSSLD"
     gene            complement(27799..28449)
                     /locus_tag="PLF66_12270"
     CDS             complement(27799..28449)
                     /locus_tag="PLF66_12270"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HMZ64865.1"
                     /translation="MVKIIIFLLMSFNFLQPQDLKKKDALDTKIQNYLENKKGSWSDF
                     NVPFEDGRALEKIILKNQYKNILEIGTSSGHSTIWLAKAASQTGGKVITIEIDKGRFE
                     KAKKNFEDAGVSHLIEMHLGDAMLIIPSIKTNFDFVFSDATWSTQPADGYTKFFKLCE
                     PKLRIGGMFTMHNVTDGYGDDGRLMKYIEILENYEMHIITVSNHGILVSKKLKERE"
     gene            complement(28437..29411)
                     /locus_tag="PLF66_12275"
     CDS             complement(28437..29411)
                     /locus_tag="PLF66_12275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64866.1"
                     /translation="MNIKIISLILLTLLFSGEIMAEYKIENVSFLSKGKKLVGKLLIP
                     SEKPKGAFTILGPVAFAKEQSPIQYASRLAKEGYIALIFDPRSYGESEGEPRRFENRK
                     WKVEDVQSSVDYLLTRKEISPDKIFALGICQGVNWTLEASTLDKRIKKVVLVASHLLT
                     PETAHRYVGGEEGVKNKLNQARLSKEKFEETGKVDYIPIVREFDLDSTALLFPKPIAE
                     WYLPWNNRAPYFGFRGSWENKITHMSELDIWGHDVTPIVKQLQSPILMIHSDNAATGP
                     DIPKKIFELIPSKEKKLVWFEEKQFQFQFYEECITIDKVVWEIVNWLK"
     gene            complement(29497..30354)
                     /locus_tag="PLF66_12280"
     CDS             complement(29497..30354)
                     /locus_tag="PLF66_12280"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HMZ64867.1"
                     /translation="MNTMFPIYRIQKFTNQDSEIWVSRFEDMPEIAEIEFPHKHNFYE
                     ILWITKGRSKQSIDFQNYKINPNTLFFISPNQLHHFEDYDNISGYCIMFTEGFLLGPI
                     GNKNFLNELLFMNHLYRNPYMHLTKENSKDLAPFFNLLLNEFNLAKNSDIVSSLLFVI
                     LKKIQYYFQKNISVFENLQIQKYTEFVELVRKNVKRYFTSKDFSERLFISQQHLNRIV
                     NKITGKSTGEIIHEIKIIDIKTELLYSEKSISEIAFEFEFEDLSYFTKFFKKITKLTP
                     SEFRNQKFK"
     gene            complement(30647..31390)
                     /locus_tag="PLF66_12285"
     CDS             complement(30647..31390)
                     /locus_tag="PLF66_12285"
                     /inference="COORDINATES: protein motif:HMM:NF020795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1963 domain-containing protein"
                     /protein_id="HMZ64868.1"
                     /translation="MLLRTRFQIVESPTEILEPVTKFGGQPVWIGQPLWPLSPETSEQ
                     MLFLGQIALDEKLFPDSKGTIVYIFFSAESEPVYNEAFAVVIQTPETVYTNGNDIEFI
                     SEATGPAIYELQQLEEDEDEEDNRAVYREYRVILEAEENEDLIPLEERYTFDNLDYDT
                     GFQFSRPELAGNKIGGQPLYIEGLDTPPEAFNSEEWLLLLQLAPTEGYWNNLQPNFYP
                     FHLELGEFGILTVFISRDYKRIEWYIQQP"
     gene            31698..31922
                     /locus_tag="PLF66_12290"
                     /pseudo
     CDS             31698..31922
                     /locus_tag="PLF66_12290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002078171.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
     gene            complement(31985..32422)
                     /locus_tag="PLF66_12295"
     CDS             complement(31985..32422)
                     /locus_tag="PLF66_12295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015679783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HMZ64869.1"
                     /translation="MLVYLDSSVLIKKYFQEIGSGYIDQIWDESDYIAISQVGYSEIL
                     GTIHKKQKMERFSDRVKNQINKAFLEDWDSLIKVNVDTTLNSELIRIHTKYLLRGFDA
                     IHLATGIILSKQFEKDLQFLTADTNLQNSAEKEKLDIGKYVWK"
     gene            complement(32422..32700)
                     /locus_tag="PLF66_12300"
     CDS             complement(32422..32700)
                     /locus_tag="PLF66_12300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HMZ64870.1"
                     /translation="MILVGVRDLKAKLSHFIHQAKTGNEIIVTEHGKAVARLIKEPTG
                     KNSLTEKLYRLAEKGTIELPTRNRTKKKFVSLKTKSKKSASDLLLESR"
     gene            complement(33021..33491)
                     /locus_tag="PLF66_12305"
     CDS             complement(33021..33491)
                     /locus_tag="PLF66_12305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64871.1"
                     /translation="MKNIPYLLLLYFLLNCASSGYNYKYSKSLSNIANPNETQFRVLP
                     GSPLYPTKDLALLTLDPYIVLLSLDDEESFPYGFEFVVLKPGNHKARIRFSSPNFRSN
                     SAVTVNFISKPNQTIFFYVLNRQELAGTQKQRALMVFFQKQNLHLHAIDSCNET"
     gene            33612..33791
                     /locus_tag="PLF66_12310"
                     /pseudo
     CDS             33612..33791
                     /locus_tag="PLF66_12310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010413974.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
     gene            complement(33852..34409)
                     /locus_tag="PLF66_12315"
     CDS             complement(33852..34409)
                     /locus_tag="PLF66_12315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64872.1"
                     /translation="MLSPAKINLGLKILFKRPDGYHELISVFLKLNWGDDMEFISLPE
                     QKMSLESIVELTGRQKEDYIAVSEQGNISKNILHKAYVRSLDRNPNLPGVHIKLTKRI
                     SPGGGLGGGSTNAASLLKFLFPEETQNPNPDFLQFASGIGADVPFFLGDGHSLVTGIG
                     EKNFTPFSSKHLFMKDSQSFFTLSL"
     gene            complement(34409..36652)
                     /locus_tag="PLF66_12320"
     CDS             complement(34409..36652)
                     /locus_tag="PLF66_12320"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HMZ64873.1"
                     /translation="MFKTIFFKLTYRLELFTYLVNVPVAIYFSAITGTLKETHEFVYL
                     SVGSAIAGTIGACIGIGWRWFFLKREIGIIQNPQLPDNLLRQSKIKLLKYPLMEPIVI
                     TLRWIFAAALAHVIFDIFAGKVHSELHMTYPFLMVLMLPLAYISFLYISEITIRPVYQ
                     TETFKNIDIDFHSIPQFSTLKRISFSMIALMLLPLVVLGYMLTGISFGTIKIEDPFFH
                     LGIMVCLFSIPVALSAYVCTKSIKNSIHDVSHVLSQLGKGQFNIVCSPSAANEFGRQA
                     HHLNQVIGRLNSMYSEINNLNSGLEKKIEERTIELKSSLDEVSKLKLQQDGDYFLTSL
                     IVNPLAYNEARKSDCKIEFFMEQKKKFRFKSKEYQIGGDICIADVIELKDKIYTVFIN
                     GDAMGKSIQGAGGALVLGVIFKSIVSRTKLVRSNQNIFPEKWLKNAFLELQNAYESFD
                     GSMLISVVMGIIENTTGMMYYINAEHPHTVLYRDEKTSFLEEGSALLKIGIKGLHNHI
                     TIRTFLLYPGDTIFIGSDGRDDILLRNDSEMKLFNEDETLFLKIIESEKGDLSRIINS
                     IKKTGELSDDLSIIKIQFFNKETYPEAETSVIHEHIGKARKFTFEKMPNKAEAELRLL
                     YEKYPYHKSSLIALINFYRTQSKWSEAVKYTEELSMQEPWNNVLLLKAGYMYFKSGQL
                     NLAADYLERLKLRGTADTRCLLLLVKTYLKTNNPKRARKILDQLLYIDPNNKKGQELL
                     RNFKDYC"
BASE COUNT        11963 a         6235 c         6023 g        12542 t
ORIGIN      
        1 tatattcttt cttttgtaca atccttattt taggcgactt gacaaagtcg cattagaaat
       61 agatctgctt catagaaaaa tgagagtcgg agaagtggtt gtggaggaag gggaagtaat
      121 ctcaattgat ggaaatagtg gtgaggtatt tatcggaaaa atcgatacag tcgttcctga
      181 tattaaagat ccttatctaa ttaaattatt acactgggca gatgaaattc gtaaattaga
      241 agtatgggct aatgctgatt atccggtaga tgctcaaaga gctagagata atggagcaca
      301 gggaatcgga ctttgtagaa cagaacatat gttctttcaa acggaaagac ttcctactgt
      361 tcaaaaaatg attttagcaa aggaagaagc aactagacaa gaagcgttaa atgctttact
      421 tcctatgcag agagatgatt ttattggttt atttcgagta atgaacgggc ttccagttgt
      481 gattcgttta atagaccctc ctctacatga atttctacca accatttctt ccttattact
      541 cgatttatct gatttaaaaa tcagattgct aagagaatca aacaatctag ggcaagtgga
      601 tgctatctta gccacaatca atgagaaaaa aactctactt gaaagagtag aagaaatgca
      661 tgaacaaaat cctatgcttg gcctaagagg tgttcgactg ggaattcatt tgagtgcatt
      721 gactagaatg caagtcagag cgattataga agcagcctgc acagtaacaa aagaaggaat
      781 tcatgttata ccaaagatca tgattccact tacaagtcat gtaaatgaat taaagcaaca
      841 aaaggaaatt ctagaagaag aagcaaagca agttctatct gagcgaggaa tgaaaatttc
      901 ttataaattt ggaacaatga ttgaaactcc aagagcagca ctcacagcag atgaactagc
      961 cgtagaagct caattcttct cttttggaac aaatgattta actcaaacta cttatggaat
     1021 ttcgagagat gatgcagaga aaggattttt aatcgaatac ttagagcaag gtattctaaa
     1081 agataatcct tttgcgacta tcgaccaaag aggtgtagga aaaatcatgc aaatcggagt
     1141 ggagctggga agaaaaacac gcccggattt agaaatagga atctgcggag aacatggagg
     1201 agagcctgca tctgttcatt tctgtcatac gatcgggtta aattacgtta gttgttcccc
     1261 tttctgaatt ccaattgcaa gacttgcagc tgctcaagtt gttctaaaag aaaaaggagt
     1321 gaaagcaaga taagaaatat agtcagcttg atgtaatatg tttatctgtg ttcatcatat
     1381 tttaaaaaat attgcatcaa gctctcgtta aataaaaaaa cttagttcag aaaaaagatt
     1441 cactttattt caatctatag gctatgtttt tacgctgagc aagacttcag caaaaaaata
     1501 ttagtatctc taacatccag aagcaaagaa ccaaacgtta ggctttccag aatcatcgat
     1561 agcctttatc caaccttccc actctttttc gatcacatca tccccagctt cgcaaaactc
     1621 acctctatga agaagaactt ttacttttaa ccattgccct tttgtttctc ttgaaggaat
     1681 gatttcataa tttgttttca aaatctttgt caacttttca cttttagaat ctggactttt
     1741 tcgcaaattt aatcctattt tgacagaatt atacaatgtt tctttctttt gaataattcc
     1801 tatccatgtt tctgaataaa aattcgcttt ttttaaatcg gataatttta caaagaatcc
     1861 tagactcaga tcttttggat tcttatctgt catatttcgc acagtatctc ttaatatgcg
     1921 aacgaatcct tttttttgtt catatacaat aaaatatctt ccttcataat tcagactaat
     1981 aaaatcggag aatttaattt ttagatattt ctgacctgat tcttgaatac ataattcccc
     2041 tttttgaata tcttcaaaat tctctttagc gtaaacaatt gaatactcaa aatcatgcac
     2101 aaactttcct tcgtcggtta tcgcatacaa agggatttcc ctatttggaa aataatatcc
     2161 ttctgttctt aatttttctt ccacaatggc gccaattcca tctggtgata taggtaaagc
     2221 taatacagaa aaatggaaca gatataaaaa gaaacaaaac ttataaataa tttttatttt
     2281 catggcatta acatccaaac gcgagtatga atgtcaattg atttcttaga agtaaaatta
     2341 aaaattagag atacagagaa atttcgacaa ataagtatta gccactaaat gaaaaaaata
     2401 aatttgtctc attttcttta gaatcaattg tgaagtgttc cgggagcaat ataatataaa
     2461 ataccaaata ttataagctg aatcggagaa gtaactatat caaagggaac actcattaga
     2521 aagagtggag aatagatata gtttggatct ttactaaaag attttatttg tatatattga
     2581 tttctatcga tagttcttat agatgaggca agtttaaaac tagatctagt aaaaccattg
     2641 tattcaatta taattattgg cattttcatc tttcgaattg gaatttcttt taacatggag
     2701 cttgctagat atttcttaca ttcttctttt tcatagagtt tcttccaagg atcttcggca
     2761 gaataaaaag ctccctgatc agaaaatata tgtcttccaa aaagaaaaca ttgtccggac
     2821 ttatcaatta atataattct attcctattt tcattgtaat atgcactctc taaatcaaag
     2881 ctataaagtt ctacattcgg atacagcaaa taggatgcac tatcgatagt cgatagatca
     2941 agtaaactat ttcctttatc aaggattggt agccatgttt ttggaatcga atcatcgata
     3001 ttgagcttta taaatttatc tgtcttttcg ttacagtcaa aaatatcctt tccatggatt
     3061 tgtctaaaat gatcatccaa attttcctcg gagcatctta gggactcaaa gaaaatatct
     3121 gatttcaagc gtagacggag tcttttggat tcgtccattg atacttcttt cactatttca
     3181 ggatccggct taatcgaata aggatagacc aagttgtata cagacatact cgcacaggag
     3241 gagtaaaata taccaaggat aaataaaaaa gaaattcgaa tatatttaag aattagagga
     3301 gccatttctc ttattttatg gaccgcttcc aattgtcagc cgaatgtttt tcttctaaaa
     3361 aaatattttt tataatacat tctcttttag atgagatttt ctgttatagc gcctatcgtt
     3421 attataaatc aatcaaaatt atttatattt cttttcataa aactcattta gcttatcaaa
     3481 tgaggaaatg gattctatcg tttaatagct ttgacatcta tatcctacta aactttaatt
     3541 taaaaaaaca tagaactcaa tatgcatcta ctcctctttg attttgactc agtagaatca
     3601 ggtcaaaaac tgaaatcatg attgaactaa gagaagaaat agaattagaa tgaaatgagt
     3661 attcaaccta tcgatccact tgtattattt acagacagaa tttttgaacc attcaataac
     3721 tcatcgcagg aatatgggga cgaaagattg tatgattttt tattgaatag tcaaaaactt
     3781 ccgatgaatg aagtttttac gaaattgatt gcggatgtcg accctttctt cggtgagaag
     3841 ataagatgat attactgtta tcagaatcga gtaataaatt taaactctca ctctaaaaat
     3901 tcagtgattt cattctctaa ataatacttc gcccttttta aatagattag agtagcagta
     3961 tcattcggat tgatttcaat taccttttcg aaagctatga tagactcccg aaacttttta
     4021 tcatagtaaa atgtaattgc atcttcaaag aacttctttg tttgtgtttt tagctcaatc
     4081 atgaaattag gctgaccatc gtaaatttca taaattacag tatagtctga ttttcctttt
     4141 actttgacac gatcgataaa gcgatagttg taatcagatg gattattaat cttttttaaa
     4201 ataatgtcgc tcactagaat agatgcatca tatacttttg tgagtccttc aattcgagag
     4261 gctacattca cagagtctgc aattacagtt ccctccattc tagcatctgc tccaataata
     4321 cctaacatta aatttccaga atgaattcca caaccaattt tgatcggatc atatccaatc
     4381 tttaatctct gttgattgta tacacggatt tctttttgca tatcaatagc ggcgtgtatc
     4441 gcatcttcta tctcacctgg aaaaagtgcc atgattgcat ctccaatgta tttatctatg
     4501 aatccattgt ttttttgaat cactggactc atacgctgta agtaagaatt taaaaaatta
     4561 aaattttctt taggcgttag tttttcactc agttctgtga aggaacgaat gtcagaaaat
     4621 aaaatcgcca tatccttttg gacttgatcg cctaactgaa tatcaacaat gctgttttta
     4681 tttagataat ttagaaattg aactggcaca aaacggctgt aagcctcgtt tgttttactc
     4741 aaattattag atagaatttt aactgatttg aatgcattgg agaaaatctg agataaaata
     4801 aaagactgag aaaaaataaa aagaaagacc ccgaatggaa ttaaattgcc attgcctgtt
     4861 aagataatct cgttattata taacaaatca tttataatcg ctaaagtaaa aaataaaaag
     4921 cctatcatcg cagcaataga accttctctt tttttataga tcgctaaccc gattacaaat
     4981 gctacatata aagtgttaat aaaagaaact acttgaaaga gaattaaact tagagagaaa
     5041 aaatatggtt gggtaaataa aactacagtt gttaaaagta aataaaaacc tactgataca
     5101 aaaagaactt ttctattaaa ttcctcgcga aaaacataat aagtaaatat agaaaaaaca
     5161 ggagcggata aatagagaga catatattca aaacgataca tccatgccca atctagattg
     5221 gggattacag acgttatcca cacatctccg acaaaacaga gtcgtataga aattaaaaca
     5281 caaaacgcac cgaatactaa agcggcaata tcttcttttc tcaagaaaaa tagaccaaaa
     5341 tgataaattg ccatgatagc aagacttccc attaaaaaga aaatatataa agaattacta
     5401 gtttgaacag attgaattga ctcttcccag cctaaataaa cggagtcaaa tcgcatccca
     5461 cctttcctat ggtgaaagtt agaaacatga atgattatgt gtgtcgattg atccgttact
     5521 acaaaacttt tcgttcgatt gatgtattga ggtttcattt catcttttgt ttctccaact
     5581 tttccatttt cagaaatgag ctcattgttt acataaagtc tatacgcagt tcccatgtgt
     5641 ggaattttta atgttaggat tttcccaatc cattcagaag gcaaacgaag attagccgca
     5701 taggtagcat gaccatatcc gcctaccttc tttccttcaa aaataaaatc attccaagaa
     5761 ctcggaactt ttataaaaga ttttggttgt gtattgtttt taatttcatc tggagactta
     5821 aattctttcc aataaaactg ccaggctcca tctagtttca aaaaaccatt ttgcataaag
     5881 gtttgtgagg aaatattctc atcgctcagg tttaaaactc cctcttgtaa aagtggattt
     5941 tgttttgaat ttgaactccc cttgcaataa aatagaaagc tgaataatcc tagaaataga
     6001 attgaaggaa gaagagaagt caatccccga aattttcctt gttttaaatg catgaatagt
     6061 cctttttatt ttgagatgga aacagaaaaa tacaaataat accatataaa atggtaactc
     6121 actttacgca aaaggaaatc ttacgttttt tttaaatttt cattttacct atagaaaaat
     6181 aggtttcaga atagagacag atctcagata ataataaatt cagattgcgt tttataaata
     6241 aggattttga tttgaatgag atatgcgtta gtttcttata ataactaata tttttacttt
     6301 atcacttctt attctaattt aaccttactc aagattcatt tataagaaag gaagaaaaca
     6361 tcgtttaata tacgttttct ccttgaccta ctcctctcca tactttttat tattcatggg
     6421 aaataccaaa acttatttta atataataaa tcttctatcc ttcaaaactg taactatatc
     6481 tgataaaata tttcctttat atattattag gtttgctcgc taaatcaaga atttgagaat
     6541 aagcaaacaa gcattgcaga tcgaatgaat cagttctttt aaatttcata tagaatattc
     6601 tagcggctat cgttaatttt aattccaact ttttctacaa tcgtatctct ttcattctta
     6661 caagtgaatt ttttatcgca aaaaaggaat aggagtcttt tgggataaga tgttttgaaa
     6721 taaacttggt tcaatttcta tcctaaaagg acgctaggcg aaatgtagat tgtagaagtt
     6781 ctcgaatgat atataatcta ttaattctta aagtcttggg gaaggaaaat tggaattttt
     6841 gcgaagttta ttttttgaaa atctttttcg ttataggtaa taattgcatt tgatttaact
     6901 ttacgtgccg ccacaacatg aaataaatcg taaacgattc cgccagaaag acctaaagaa
     6961 gttatttgca agataatttc tttataatcc ttttcttcta aggcgatcac tttaaagttt
     7021 ggataaatat ttttctcaat gatctctttt atctctaagg gagatattct tgtttttagg
     7081 ggtagttttg aaaatacaga ataaaattct aggagcgcat gagcggaagt aaccacttct
     7141 attttttgat tgctgatttt ctctagaaaa gaaaagcatt gcttatgatt tggatgtgct
     7201 tccgcaaagg caggtattaa aactgaagta tcaaagaaaa gtcttttgat catagctgac
     7261 tatcgcgttc tttgcgtgct ttttggatta gtttgacagg atcagtctcg ccaacccaat
     7321 ccgtagagac aacagtgaat tttcctttct ttttgagagt tgatttttct tctatttttc
     7381 tgactcgaat ctcgagacca tcgattaaaa tttccaattc agaatttgga ccaataccta
     7441 gttgatctct aatttcttta gggactacga gtcttcctac tttatcaatt actactgtca
     7501 taatgactcc atactaattc ccatttacca attgtcaatg ggaattggca attttacata
     7561 gcaacacaag ttcgaaaggt taaattaatt ttgagttttt aaatcttctt agattagtga
     7621 agtgtgtgta gtcaatttgt ttgaatagat cctttcatca aaagcagggc tgcaagtctt
     7681 tctagcggct atcgttaatt ttaattccaa ccctttctat aatcgtatct ctttcattct
     7741 tacaagcgaa agttttatcg caaaaaagga agaactccta tacaaatggt ttagagttac
     7801 acacttggag ttataataca atgccttcca aaatttttcc tatttaaata acgatatgat
     7861 tcatagtcaa tatcctagac actggattta ggaattttct aattcatacc tttttttctc
     7921 ttcatagaat ttgttaatta catcgcgacg tttgtttcta aaaataagtt tgaaattttc
     7981 ccaagtggaa tcaaaaaaat ctgctcctcc attttcaatc gaatactgaa aaaactcttc
     8041 tgcttttgga atttctccat tttgccagta aaaaattgcc aaattccaaa atgcgttagc
     8101 cgatgtgttt tttgatttac taagagcaag ttcccaatct tttttggcaa actcaaagtt
     8161 ggggttatca ctattaaccc aagaatttcc cctttttaga taagactcaa cttccttaat
     8221 tcgatcctca tccttaacac cgacagcaga ggtgaaaagt ggaatggaaa atggtcttac
     8281 acgaggagaa atagtatttg cgatattgtt tgaagcgatt tgaatggctc gcaagtgagc
     8341 ttctgatcta gaaggacagg aaatattcat agattttcct ctattggtta atatggcagg
     8401 ttctgaattt gaggctacta acatttgtcc gctagatata tgaatcaact ttgcatttgc
     8461 taaaatagta taccttagtg ttttttcata agtaattata ttacgactaa tatttttcaa
     8521 acattttcca tctttgtccc gaagcacaca agatgtttct ttttttgaac tggaggaaag
     8581 tttacagtca tactgtggtg gttcgggaat ttgaacataa agtaaagctt gcaccgatag
     8641 ttctgatcct agggaacgaa tttcatctcc cattttttga gcaaaactca tttcctttaa
     8701 tctttgagat cgattgtgaa tatctacaat tttaaaatac ttaaaatcat tgagcgcaga
     8761 ttctattccc gaatgaatcg tttttttcca atcatcctcg gaggaattgg gaatgtcatc
     8821 tacaaaaatc cccaaagagc gagccccttt cattgtctca taaaaattgg gtggagctga
     8881 ttctactact ggaattttcg ctgttgtcac acattgaaca aaaataaaaa tcgataaaaa
     8941 gaaagggtaa aatctgtttc tttgttttgc tttttcttgc attgtaaatt cctaaagaat
     9001 atctatcatt tcagcattga ctttttttct agcgcttatc gctaatttgt atccaatttt
     9061 ttccataaca ctctcccatt tccatcttac aagtgaaaga cttcaagcaa aaaaagattg
     9121 tgggtctttt agaataaggt gtttggaact agatgtgata aaatatttta attagaattt
     9181 tttttaaaag gtctcctttt tcactctttt actaaatata aattcggcac aacaagagtt
     9241 tatgtttatt accctcgaaa tttatttata tttattgatt gacaaaatat atatcgtaat
     9301 tatacgatta atcatggcac aaaacaaaaa gacagagttt aaggaaaata tacaaaatct
     9361 ttctgaattc gcaaaagcaa tttcgcatcc agcgcgaatt tccatcctaa agacaatcgc
     9421 gcaaaagcag gaatgtattt gtggtgaaat tgtagaggta ttgccattag cacaatcaac
     9481 cgtttctcaa catttaaagg atttaaaaga agttggattc attaaaggcg aagtcgaagg
     9541 aaccaagtct tgctattgca tcaattggaa aaaggtagaa gagtttgaaa aagaaatcga
     9601 aaagttattt caagatttaa aaaaacataa aaatgaggat aactgttgtt aacctgtaat
     9661 ttccaattgg aaaaaagttg taaaagtcaa atagcgatgg gtcagcttag gcactttgca
     9721 aaaagcaagg caatgaagat gaaattgcaa atctatttca tataacgctt taaataataa
     9781 aagaataaag tagaaaattt attgaggaga atagcaaatg aataacgtat tagagaaatc
     9841 ttttccacag gtagggattt taaaaccaca tatcgcgatt gtcgtaaaga atgtagagga
     9901 gagcatagca ttttatacaa agatgttcgg aaaaaaacca tctaaagtaa gaagtggata
     9961 cgctaagttt gatttagaat ttccatccct aaatctaact ttaaacgaat caggaaagaa
    10021 tgttccacat ggtgcactct ctcacctagg tattcaagtt agttctaccg aagatgtttt
    10081 gaaagtaaaa aaatattggg aagaacttgg attaaccgct cgtgatgaat taggtgtatc
    10141 ttgctgttat gcgattcaag ataaaacttg ggttaaagat ccagatggaa atgaatggga
    10201 agtttttgta gtcttagaag ataatcttcc tgaattcaaa ttcatcggtg gaacggctaa
    10261 tgcgtgttgc tctacatcct ctagctcagg tcaatcttgt tgttaatatg aaaattaaaa
    10321 tactaggcac aggttgccaa aaatgtgtag cacttgaata ccaaacacaa acagcggtga
    10381 accaattgca actagacgcc actattgaaa aagtaacgga tatggaagag attatgaaat
    10441 acgatatact ctccactcca gcacttgtga ttgatgagca agtcattttt actggaaaag
    10501 tgctgaagac agaagaaatt aaaacactac tacttcaata caactcaaaa tgatcgagag
    10561 gttaattcat attattctct atagttggtt tgggctcgat ccttcggctc agctaactat
    10621 agcgattgat ttctttttct acgattcaat taagatttta attcttttgt attcggtttc
    10681 tatgctcatg aattttgtga atacatattt tccaatcgag aaaattcgta gttttctaac
    10741 aaataaaaat ctcttcggat ttgaatactt tctggcatcc ctctttggag cggttactcc
    10801 gttttgctcc tgctcgtccg ttcctctttt tattgggttt gtaaagggtg gaattccact
    10861 tggtgtaacc tttgcttttt taattacatc tcctcttgtg aacgaaattg ccgtagccat
    10921 gtttcttgga atatttggtt ttaaggtaac tgcgatttat gttgtgagtg gaatattgat
    10981 tggtatgact gctggtttta tattaggaaa cttacagcaa gaaaaatttc tcacggaatg
    11041 ggtaaaggaa atcctaaaca aatctgtcgc aagacagaca aattatattt tagaaaataa
    11101 aagttttcgg gagcgaattt tagaagcgtc taaggagtca ttcggtattt taaaaggcat
    11161 tgttttgtat gtattcattg gaattgcttt cggtgcggct attcacggtt atgttccgaa
    11221 tgattttttt atccagtata taacaaaaga aaatctattt gcaattccag ttgctgtttt
    11281 ggtaggcata ccactctatt caaatgccgc ggggataatt cccattatcc aagtgttagt
    11341 cgcaaaagga attcctcttg gaacagccct tgcttttatg atggcagtgg tcggactttc
    11401 tctaccggaa gcaatgcttc taaagaaggt tatgcaaatt agactcttat tgactttctt
    11461 ttttgttgtt ggagtttcca ttatattact tggatatttt tataatttgg tgctttaaga
    11521 atcttaggag tggtatctaa tctttctagc gactatcgct aatttccata aagagacatt
    11581 gtcctttttc aaaggatacc aaatatcact cttatcagat acttctcctc gagcaaccga
    11641 accaaaaact cttaagtcat aagcccccgt gcttttttac aattaactct tttcgctaac
    11701 ttcgcgacat aggctaataa attcatagtg aataaaattc ttaaaatcta aacaaaaatt
    11761 aaaaattctt tcttctcaac tttttcggga gttgcacaat aaaaaataaa aaaaataaac
    11821 aatagtgtaa tggaaaaaaa tattctatga atcatcactt attaacagtc ctataccaat
    11881 tgccaaaagt aaattctaat tttaaactag atgacatgct cttggcaatt gccctttttc
    11941 gaacatatcg ttacccacat cttttaagat taccaccgat atcaccatcc gagaagcgaa
    12001 gatgatgaac accgataata ttttaattag tactaattgt tacgatcgta tgcatttaga
    12061 agacgaatat cttgaatcct tttaaataaa aacgtgatta aactaacgaa tatcgtaaaa
    12121 taacaataat aaaacctatc ggtgttcaga ccccccatat cgctccaagt attcgcgact
    12181 aacgcctttg catttccttg gtggtaatct ttaaaaaaat attgtgggta aggatatgtt
    12241 ttgcgaaagg ggacttatct tacagggcta cattcttttt tttcttcaat ggtacgcctc
    12301 acatggttat aaatattaaa cgcataacgt cttgtgaaag tacatccatc gtctgcaaaa
    12361 cctgcgtcta tatcaatctc atatttattt agccaggatt tccaaggttc tggtttctcg
    12421 tttaaatctc ttgtgtctag gattagatgg tttttagaat taaaaacaag ataaaaacaa
    12481 tcaagggtgg cgtacattgc attacactca tacccatttt ttgaatcggt aagaccaatg
    12541 aagtaatttc ctgtttctga attgacttca tttattttta cttcgaatcc acaaattgtt
    12601 tcgtcctttt ttgaatatgt taaaaattca gaaaacgcat aaccaatatt agactcataa
    12661 gaaactttga aaaaagtccc ggtcttgcct tctgattcta caggaacttt tgagtcttcg
    12721 ataaaatcaa ctaagttctt atagaggata gtcgtaataa cctttgcgga taagcttggt
    12781 tcttctctga gtcttagccc agccttcgct tctacaatca aagaagttcc cacacattct
    12841 ccttgcattg atttagacga tttaaaattt ttttggactt cgatagactt agcaggtgtg
    12901 gaagagagct gttgttcttt ttctaaatca ttgtctttct ttttacaaag acctaaaaca
    12961 aaagctagga ttaaaaatag aataattttc tttatcattg gaacctcctt cgcaaggtgt
    13021 atattttact gccgatattt ttatgtcaag ctctatcctt tcccagtcga tccaaaccca
    13081 actgttccac gctttgtatc caaagaaatc gtctcgacca aattccactc catatcccaa
    13141 gagcggcaaa caagaatttg tcctatacga agtccgttgg taactttaaa cggagcttta
    13201 cttaaatgca aagaagaaaa ttttccccgt ctagttcaaa acatctcttt atgaaagact
    13261 tgcaaacctt tttgtattaa gcctttaact tgtaagatag aaacgggaga gaataatgga
    13321 ggaagttcgg tgcaaattcc tggaaagagg agcattttta attcttgtca aaaatttaag
    13381 ttttaagaat tttattaact atgaacttat tagcctatgt ccgaagtcag cgaaaagagt
    13441 taattgcaat tgcaaaaaag catggggctt atgacttaag agtctttggt tcggttgctc
    13501 gaggagaaga gtctgataag agtgatatcg atattttggt atcctttgaa aaagggcgaa
    13561 gtctctttga tgaagttaga ctttttgatg aactaactaa tcatttaaat cataaaattg
    13621 atttggttgg tgaagattcg ctttctcctc tcttgaaatc aaaaatatta aaggaagcaa
    13681 ttcttatatg aaggatgatt tgatttatgt aagacatatt ctaaatgaaa tgtcagacat
    13741 tatagaattt tcaaaaggtg gagagaattg gttttttcag gataaaatga ctcaaaaagc
    13801 aattatacgt tctctagaag taattggaga agctgtcaaa aatatttccg cagacttcag
    13861 agaaagtaat cctcaagttc catggaagga catggctggt atgcgcgatc gattaattca
    13921 tggttatttt gatgttagcc ttgagcgcgt ttggaacgtt gtggaaaata taatcccatc
    13981 tttgaaaaaa caaatggaaa atcttttata gaggctactc ttcatttgtc acatatccga
    14041 caaggcaagt tctaatagaa tgtgatggga attagataaa ttatccatct atttgaaacc
    14101 atttctttcg aaagacactc gcaattctac acctttgctc tcgtatctga gctagaaaac
    14161 aaatccaata tcccgacatt gctcatagga taagatcgga tcaattcccc tcactcggga
    14221 cgaatgctaa gcggaagcca ttaaagatat tccacttagt taattgttga taaaaataaa
    14281 agaacgggaa aacctagcat aatcaatcca agtgtttcca aaagtaagtt ttcgatgcga
    14341 gcagaaaacg aaaatcgatg cctcttgggg aatttctctt aacatgcccc gttttccaaa
    14401 ttgtaaggtc atacagcttt tgttgtaaat ttagttcatg tttcattttt caaaaaaaat
    14461 ttgggcttcg cccatccaga aagaccagac ttttctatgc ctataggcat agaaaagaca
    14521 gaagttcaga ttcacagact cgcacataac aacgctaatc cgctgaaaag aggaggcgaa
    14581 acccaatgcc gtagctgcga agatcgggcg aatatttgat gcgataggag gaacgcatga
    14641 gatcgacact atagtaccac gagccaccac gaagtacccg attcgaactg gaagaaggtc
    14701 ctgcgggatc attacttggg gattccccat agtatccgcc atgataccaa tcctcacacc
    14761 attccgatac atttccagac atatcataca atccaaacgc attcggcttt ttttgtccca
    14821 ctggatgtgt ctttctgcca ctatttttat cataccaagc aatggaatcc aaatcatttg
    14881 agtaatactt agtagttgtt ccagcacgag ccacatactc ccactctgcc tctgtcggaa
    14941 ggcgatactt cttgccttct ttggaattta atttttttat aaattcctgc acatcattcc
    15001 aacttacttg ctccactgga caattatctc cgcagtcttt ataattggaa ggatttgtcc
    15061 ccattaccgc cttccactgt ccttgggtta cttcaaattt gcccatgtaa aaaggttttg
    15121 taattctgac tttatgcgct ggtttttcag aatcaaaaca tttattctct tcctctgaac
    15181 agcccatcat aaattctcct gccgggatta aaacaaattc cattccaata gaatttgtaa
    15241 aattttcttt ttctattttt acttcagtaa tttccttttc tttacaacta ctttcgctcc
    15301 aaacttttcc caaaatttca caattttctt tttctgtttt tgatattgta attttctttt
    15361 ctttacaact attctcactc caaacttttc ctaaaaattc acaatcttct ttttctacga
    15421 tttcttcctt actctttttt cgggagggac tgataatttc tttgaatcta ttgttccagt
    15481 ttggatcttt tcttgtattt gccatatcag aatcattttt gatataatca aaagccttgt
    15541 atccattatc cacagctttt gttaaatatt tataagacat ttccacctga ttcaatcggc
    15601 tataagaaca ggctatattg tataacacaa attctggctt gggtgcattt agataccgtg
    15661 caattttata agctttaatg gaatcttcaa gcctatcaat gttggaaaga gagtttccat
    15721 aattgtaata ggtttcgcct aacggataag agtcgataga tttttcatat agtgtaacag
    15781 cctctttgtc ctttttcttt ttgtagagag caaatccctg ttcatccaat tccttggctt
    15841 gtctttgcaa tgtatacttt gtagaatcag atttcaattg attcaattga aaattcaccc
    15901 aaggaataga aagataatcc tttgacggga tttcttcttt ttttgcaacc tcaccactct
    15961 ggatgttcgg tttagattta tctacacaag aaccgaaaaa caatacaaaa aaaatggcac
    16021 ctaaataaag atatagttta tgaattttca taacattctt ctttcctgtc atatttatat
    16081 ttgtcaaata aatttagacg gtgggttttt ctttcaagga atttccatcg cattctatta
    16141 gtcctttcct cgtccaaagt taaacaaaat cagatattgc cgtctttctt cgccgtcgtt
    16201 gcggcaattt agtagtagcc tctctagaac gggtaaaatt ttatcctaac caaattgaat
    16261 ttctaaaagt tgcttatgaa tttcgctcag actttcatta ctacgattta attaatatcg
    16321 ggagatgatt ctttacccaa atatctaatg tttttataaa ttatcatttt tctgaattat
    16381 aatttatata cctattacaa attactgcat cttcggtaga ttggtcacca aaagttaaat
    16441 gtacccgagg attatcatta taaatttcat tactagtata actaagatgt aactttccat
    16501 gactttcgga gaagcaagtg atatctttcg aatcgcaccc tatatcccca caaccataat
    16561 gattgtcgag tacagttatg ttttttgatc catatttgaa tcgttttttt tcaggatgat
    16621 ttaaaagaat ttgccaaata acactaaact ttttctgttt agtaaattcc atgttgctat
    16681 atgtatctgc ttcttttata aaaactaata acaaagcttt tgcgtaatta ttttgttcaa
    16741 agattgtttc tatgcttgga agtgttgaaa cgaaagtttt ttttcctatt atagtctcct
    16801 taataaaata ttcaggaatt cgatttgttg attttaataa aatttcttct tcatttgttt
    16861 tcaatttaaa agttattcca ttagattctg gaattgattc aatttcttgt agttcaaaaa
    16921 tagtgtcatt aatttttaaa ttaactttat ctaaaatctc aatacttaat tgaatttctt
    16981 ttgtaattaa atttccagaa gattttatga cttcaaattc attactacca tataatactg
    17041 cttttaaata atattcttgg aaataaaaaa ccttcttttg cgaattaaat ttattagaaa
    17101 gagttgacca tgaatttgaa aaaagttcat taaatttaga ggtatccgta ataatatcga
    17161 ttgcagtcat tggcttgaca ccttcaatat ttaacttatt gagtatggtc ttattccaag
    17221 aatctttttc ttcaggattt tcaaaaaatt ttttaaaaac attttgaata ataggatatt
    17281 tatttgcaag aattcttaat cgattaatat cttttacttt ttcaggaaat tttaatgcat
    17341 gtaaaaccaa tttaatttca ttttcagtta taattctatc ttgaaaattt tcgataaaac
    17401 taagataatc ttctactttt ttaatgttta tctcagatgg aaattccttc ttgggatcaa
    17461 gtatccaatt tcgaatagaa acttctttat caaattcctc tttagttaaa agttctccaa
    17521 ttaaataatg atctgatttt attttgttat ttatcagtgt atattttcct aagtctatat
    17581 agacgttatt ccaaatacaa tttatctttt cagaacaatt aactttaagg aaaaattttt
    17641 cagatttaac attatctttt tctatttgaa agatttctct ttcagagact tgtattatat
    17701 cattgaaacg aatttttcct attttcttag catctaactt tactgagtca acaagatcaa
    17761 attcaactgg gtaaacaaaa tttaatatta agggagaatc aaatttttcc acattacttt
    17821 ttttacaatt tgtaaaatct aataaaaata aaaacaagac taaagtataa aaaaaattgt
    17881 tcattaagct acctcattga tgatattgtt acaaaactta gatctagctg aaattcattg
    17941 tgttcatttg ataatttcaa ataaaatttg tataatcttt ttttattgat ataaatttcc
    18001 cccgtttgat tttctttcta gcgctatcgc taaattgcat tcaacttttt ccaatatcgt
    18061 cttccgttta agttaccaaa ggacttagta gagcgcaaat gtttgtttgc cgcttttggg
    18121 atacaaagcg tggggcaggc ggggataggg tttgacataa aattatcggc ggtaaaattt
    18181 atacattgca aaggagagcg caatgataaa gaaaatgatt ctatttttaa ttctaacttt
    18241 tgtattaggt ttttgtaaaa agaaaagtag tgagcctgta aaagtagaag agcctactaa
    18301 aaattatttt tctacacaat gggtaaactt ctgggtcgcc aaattggaat atgccccaga
    18361 gcaaaaagca agattggctg aggctaaaaa actcaactca aaagcctttg ctttatacaa
    18421 aagtaaaaaa gacaaagaag cagttgagtt atatgaaaag tctattgact cttatccgtt
    18481 aggcgaaact tattataact atggaaattc cctctcaaat atagatcgtt tagaggattc
    18541 aattttagct tataaaattg ccttacattt aaatgctcca cgtcctgaat tagttttata
    18601 caacacagct tgctcacaca gtcgattgaa tcaagtagag gaggcatata gctatttggc
    18661 gaaagcgata gatagaggat acaatgctat tagttatata gaaaaagatc ccgatatggc
    18721 aaatctaaga aaggattctc gttgggagag taaaatccaa gagctggtaa attccaaaca
    18781 atataataat gaggatttag ccggacaatt aaaaatttcc tatagaccac gtggaggtga
    18841 tgaattcatt ctttgttcaa gtggagttgc gattactcct attgatcctt ttagtggaat
    18901 tggtggcgcc tgtggggaca gaagtgcttt tgaaaatact caatatatgt ataaaattgg
    18961 aagatggaaa cttacaaatg gagatttaga aataacattc gaaaaagaat gcagtccaga
    19021 cattattcaa tcttctagag gggacgcttc gactatgggt tgccaaggat tagctggaac
    19081 tcctaaattt aaaaagtgct cagattctat tagtagcggt aattcagatg taacgttttc
    19141 aaaagataag gtttcgacta ttctagcaaa tgatagtaaa gatatggata cagtttacga
    19201 attaaacaaa aactattcag aaccaaaaca atgtgatcca aattttattc cgaagacact
    19261 ggatgatttg aagttggagt aggataatcg agattaggcg taaaaattca tttcctattt
    19321 tctggattca tttcttggga ggtggacctc cacctttttt gtctccaccg ctggcttgtg
    19381 ctggcttctt atctcctcca ccttgcgttg gttttttatc accacctgcc tgtgggggat
    19441 taacggtctg aggatttgcg cctgctcttt cttctttgta ttttttaaga aagtatttaa
    19501 ttgtaaaatt tatgataatg tatccaacga tagatacata ccacattgga tctattttta
    19561 tattgtccat ttttactcct tccttttatt attttgaaaa tcttaggatc ttttcacgga
    19621 taacatacat tttctctttt gagggtaaat catttcgtcc gcctagctct tcgtcatgga
    19681 aataatagac gagccttgcg tagagaggct tatttgaatt aggcgcgtcg attgtgagag
    19741 aaatcaattc ctcattggga gttagcccag tatttcgaat cagtttgcga ttccagaatg
    19801 tatttccgcc aatgtttcca atgccataaa tgcgtgccca ttttttgcga tagtggaaat
    19861 gtttgtattc tggatcggtt gcaatttcta attgaatgga atgaaataaa tcacctgttg
    19921 gaagaatatg agcacgattg ttttttagtc gtagactaat atgaagtaac cctttctcaa
    19981 aaaattcata ctctatttct gaaaaatctt tttctaatct ttccggatta tgatttccaa
    20041 gtagtctatg tccatcgtag tgacagtcct ggcaggtgcg ggaagaatta gaatttttcc
    20101 attctgaata ggtattctgc atcggctcgt ttgagtagtg aattttattt ttttcaaatc
    20161 ttggaaaatt aaactgatgg caattttcgc aaaattcgga agtagaaaga gaatcgaatt
    20221 ttataacttc gtgaaaatca tttttaaaat tttggaagcc caatactttc ccctctctca
    20281 catgacagga agcacaatta attccttcgg ctaataagga tttatctgaa ttgctattgt
    20341 ctaggaattc ttttttctgt tccgaaaggg gagcgtgaca gtttacacac cattcctgtc
    20401 tttctatttt aaatgcattt tggaaaagag gatcagtcca agctttggcg tgcattccat
    20461 ctttccactc taaatgagtc tggttatgac agctttgaca ctctttagag gataagctcc
    20521 gtttgccttc ccatcccgtt tccacggaag aaactccttt tgggaatagt ggaggtttca
    20581 tttcgttgca gtagagagaa aaaaataaga tgattacaat cagaacggat ttcatattca
    20641 ataggatttc aaaaaaaaca tttatctagg tttatcatca tggcaagcga ttaattgtgc
    20701 cttcctaaaa ttcgatatta ataacgatcg ttattaatta aaagattgac tttaattatg
    20761 ataacgatta ctatgaataa tgcttaagta aataagcaca ataaatagga gattttaaaa
    20821 tggataaatt tatttttata ttcagaggtg gagatactca tatttccaca gctaattcac
    20881 aagccgcaat ggacatgatt caaacatgga atgattggat gcaagggctt gcggaaaaag
    20941 gaattctcgc gggaggcgat gcacttcaag tatcaggcaa gcatgtgaaa ggtccaaaaa
    21001 aagtagtttc agatggtcta tatgctaaag gaaatgaaat ggttggcggt tacttaatcg
    21061 taaatgcaaa tgacatgaat gatgcagtag aaatttcaaa agggtgtccg attttgattg
    21121 aagacggtag cgtagaagtt cgcccaattc aaaaaaggta aatgtaaatt ttggaggata
    21181 cttcaaaata gccggaaatc aataaaacaa ttgaccatct tttttgaaat gagtaaggaa
    21241 agatggtttc tgttttaaag ggtatctttt ttcaaagtat ctgaaagaaa aattgaattt
    21301 ttaatctaag cttaaatata attctagtaa aaggggaaat cggaatgaaa actaagtctg
    21361 gagagaagga aaagcttaga gaaaagatta tagatcaatc tatcaaagtc ataaaaaaga
    21421 aaggtatttc tggtgccagt gtagatacta ttatggagtc ccttggttta acgtcaggcg
    21481 ctttatatag tcatttctcg gggaaagatg atctattggc actaagcatt gaaaagcaga
    21541 tcaactcact aactgacttt gttagcactt taatagagaa tgataggaag gaagggttga
    21601 agaattttat taaatattat ctaagtgaga agcatgttcg agatttagaa aatggatgta
    21661 tctttgccgc tcttatatca gaagttgcac aaggacctca gaggataaag actgtattcc
    21721 atgctggatt agataaattc tattcggctc tagaaaaagg attacctaac gactataaag
    21781 ataagagtag gacagttcaa tttatttttt ctacgatggt tggctcccta agcattgcca
    21841 gaacaatgaa atctgggata gagagagacg actttttaaa caatgtcttg gcatctgttt
    21901 tgcaattgct cccataaaaa ttttcacccg tcttattcaa aaaatatttt taggaaagac
    21961 ttgcattttt ttcatcagtt tacaaatcca gatacgatga atatccaaat tggtaatttc
    22021 ttatatgttt ctattttcat cctactataa catataacgt tacaagattg aatattttgg
    22081 taaaatccca agaaaattca tttgtttctt ttgatatttt tctttctact ttctaacgcc
    22141 atcactagtt tgcatcgaac ttcctgcata atcgtctccc atttctatct tacaagtgaa
    22201 tactataaag cgaaaaaggt ttgcgagtct tttagaatga gttgttttga aggaaacggg
    22261 gtaaaatttt agctaattaa ctgattgaca aaaagaattt ttgtattaca gatgtgatac
    22321 aaggattgag aaaatgatta gtcttagatt accgcaagaa ctagaaagaa aattatccga
    22381 agttgctaca atggaaaata agagcaaatc ggaagtaata aaagaatctt tattatacta
    22441 tataaaccat ctttcccaga aaccatctgc ttatgatcta gggaaaaaat atttcggtca
    22501 atacaagagc ggtaaacagg acaaatccgt taatcatcaa aagtatatca aagacgctat
    22561 actcaaaaaa caaaaaaact aaatgataaa aagcctgatt gatactggtc ctattgttgc
    22621 tttttttgac gaatccgatt cttattgtca ggatattcga aatttcctga aagattttaa
    22681 aggacatttt tatacaaacc tggcgataac aacagaagta tgttacctat tatctgatag
    22741 taaaaaaact agaaactcct ttctgcaatg gataaaagat ggagccatta caattctagc
    22801 gcaagaaaat aaagatttta ttttaattca atatttcatg gaaaagtatt cagatcgtcc
    22861 gatggatttt gcggatgctt ctttaatcgt tctttcagaa tctcacggaa taaaagatat
    22921 actcacacta gacagcgact ttttatttta caaaaataaa aaaggcaagt cacttaatat
    22981 tttgaataaa gatatgattc gatgattttt ctatctatct agcagctatc gcttaattca
    23041 caccaaactt atccgtcaat tatttcccat ttctatctta caagtgaatg acttaaatca
    23101 aaaaaggatt gtgaggcttt tagaaagaga tgttttgaat taaacggtgg agtattttta
    23161 tgttgaatcg attcccttaa aattttattc cgtaaaataa atagggagtt tctatgttta
    23221 ataagatcaa aaaatttact tttatcttcc ttgttctaac ctttccagtt tttgcgcaag
    23281 attcaaaaaa agaaatcgct tctgatgaaa aggaaacaac taaaaagaaa gaggaagata
    23341 aaaaggatga gccaaaagga ccaaaatggt tttctttaaa tccaggtgta acgcttgaga
    23401 gcgtaacggt ggatataacc ggcagaggtc ataatgcaac tatgacgcag ggtggacctg
    23461 gaagatcaac ttggatgttt gacgtgaaat caagaaacat ccaattcact gaaaacattg
    23521 gagtctattt acttttacac aattccgaat ttcttctaaa taatcaattt ttaccaaaag
    23581 tcgctcaggc tgatgctcct tctagttcgg aatcttctgg ctcttcctct tcaggcagtt
    23641 ctccttcgtc tgatggtaga gccaaacaaa atataggcac tcgcattcgg ggaaattaca
    23701 caatggcaat tcctgtttta ttttatggaa acgataaaat aggacgaata gggataggag
    23761 caggtcaagc caatgtttca atgaacggtt cagcagattt tcaaaaccct ttaaatacat
    23821 atgctttctt aggtctcgat agttctaaca aagttaattt tttagataca gtttctaaat
    23881 atcaatttat aactggtaac attaatccgg tgagtggtga cccggttgca acttatttac
    23941 tttccaatct ctcacaagga aataatttag aattattagg actctattat gcttcccaag
    24001 ggcttctcag aaatcctgat gtgatatctc tcttaccata tattcgaaat cccagacttt
    24061 actctccaat cgaagcttat gctcttgcaa gtcttgcagt gtctaatgtc aattttgcga
    24121 gacaaaatgt attttctttt atggcttact atgaaactcc aaaatttgga tactttcgtt
    24181 tccgacttgc ttttggcgga cctgtattta aagataatgg ttataagttt gagtttacaa
    24241 catttcattt agcgctcttt gttcccattg aattttaaca aaggattcat tcccaatttt
    24301 tttctaagtt taatattcaa cagtctttaa aattgactct gcggtttacg actatttgca
    24361 tcatcgatca gagtctttta ataaaaatct ttcgccaatt taaaaaacta ctactgcccc
    24421 gagacgcaaa gatttaattt tgttcaggag tggatctcta tagatgtttt tactagatag
    24481 catttcatcg aatgcaattt ctggcttaga aactccagaa gcatttgtga atcgaaagaa
    24541 ggcaggaggc agataatttt cgtagtaaac atataaccta tcataatcaa gcgttaacga
    24601 aatggaaata gtttcataaa ttggatactg gtaatctagg agtatccgct tcaagaaaat
    24661 acttgtcttt ctagcggcta acgcctaatt tgcatcgaac ttctcccata attgtctccc
    24721 gtttctatct tacaagtgaa tggcttaaag caaaaaagga ttgcgagtgt tttgataata
    24781 aattgttttg aactaaacaa ggtaaaatat ttattgttga tttaaaatta ttatgcaaat
    24841 tctattctgt gaaaccgcat tgattactta tgacttcata atttatattg taatcatcga
    24901 ggaagctgat gcatttaact cgaagaaaaa acaagacgtt ttttatggga gttgaactta
    24961 acatagttta ctaatcgttg gatttattca ggtttcatct ttgtctggta gaaatgaagg
    25021 aggcgtcttt tttggaaatt gtggcaatat tgactttatt gcgagagctt ctcaaacaaa
    25081 gaagtcaaaa ggcaatgtca tggggggatt aaaaaaataa ctaaaaattt aatttcttta
    25141 aattaatgat tggataaata caagcgcatt ctctgacaat tcaagaggtg tcgatccacc
    25201 gccaattaga aattccgagc attgtccaaa aggcagatga tgttctttgc catctgaaag
    25261 agtaacaagt gattcacatt cccctcggtg attgtgagtg aataatgcta taggtttatc
    25321 attccttaaa attaaatatc tataagaatg aaatgatttt tgttcatcaa ttatttgttt
    25381 tattgaatat ttcattaatg acttttcttt ctaggaattt gcatcgaact ttttccataa
    25441 tcgtctatca tttctatctt acaagttaaa agcttaaagt aaaaaaggat tgcaaggctt
    25501 ttaaaaagag atgttttgaa ctaaactggg taaaattttt gagatttgca aaattaatta
    25561 ttttagtaat cattttcttt cctgtcttat tttcaatcaa agcagaaact agggtagaac
    25621 tgaaggcagg catttccctc tccgattttc aacctgaatt tcaaaaacgt tattatctag
    25681 atgatttgat tgctggtgga agcgatggat tgggatttgg agcgattaat cgaaagtatt
    25741 cacagagtta tttcgctgca ttgttaaatc cctttgactt taagtttttt cttacaaaga
    25801 gtgattatgt atttttattt ggagcggaat atttaggtct aaatggaatg actacctctc
    25861 ggatgcagtc taattttaat tactcaccaa cgggcttttt tatgggggaa ggaagtattg
    25921 attccaaggc tagaaacgca aacactagat tatccgctgg taggaaattt gattctataa
    25981 taatcagtgg tttaattatg aagcgcgatt tccaatctga gtatgaaaaa attgtaatac
    26041 tatatgcacc aggattttta ggtcgagtga atgcagagag caattactat acttctaatc
    26101 tatttgctgg tttcagtgtt acaaaactat ttgaaaaagg aaaaattaca actgaggttt
    26161 cttttcgtcc caaaaatcag ggaggtattt ctagtgcagg aaagattgaa caattaggtg
    26221 taattcgatt tccagtggat ataccaaccg gaaatttatc gagcgaatta ctagttacga
    26281 attatcttta tggagataaa aagccaaata ttttcggagt gcggatactc atggattacc
    26341 aatatccagt ctacgagaat ttttcaattt cttttacaat agattatgat agattatatg
    26401 tttattataa tagctatcag cctcccgctt tctttcgttt tgcaaatgct tctggaatct
    26461 ctaaaccaga aattgcttat gcagaaatac tttcgagtta catcatttac cgagatccta
    26521 ttctaaatga aattaaatct ttgcgactcg gagcattagt atttttttaa tctaacgaac
    26581 tatagagtcc taatattcct gcgattccag tgcagatagc ggggatagga aaaaaataaa
    26641 aaatacttat aacggataat gctaataatc ctaaactctc aagttttagg atttgtttgt
    26701 tgctagattg aagtttctct tcagatgaaa ttaaaatcaa actgattcca gaaaccaata
    26761 aatatacact aagaccaata ttagatccca gtaataaatc aaagaaactt tgcttctttc
    26821 ctattggcat gggaactaaa gctgttttca tagcatcttc gattttgatt aattcaactt
    26881 cttccggtgg caacggcata aaaattccga tggtatgtcc taaacaagta aacaaaacca
    26941 atccagccgc aattcttaaa attatttttt tattcataaa aataaatttt tagtaaatta
    27001 atttttgtca aagtgaaatt taaaaaagga aacaaaaatt aagaacctgt atccaaacca
    27061 actcatcttt ttattccact caaagtagga cttaactcga gacaatagga aaaaattact
    27121 ctttcaattc tattccaata aaggtaattt cttcttgtat gaaactttta tctaggatta
    27181 tttttagttg ttcctcaata tctgctacaa cttgatacag cggaatattt ttttatccaa
    27241 caaaactttc agtaatctat cctctccaaa ctcttccaaa ttcatattaa gttattcaaa
    27301 tattccatct gtaaataaat atatcctaaa gtcttttgaa aaatctctca cttctgtatt
    27361 gtaatccgaa tagaaccatc ccattaattt tcttgttcta ggcagcaaat cgtgattatc
    27421 cttatgcaac aatatcatat ttggatgacc agttgaagag taataatgtt tttttctttc
    27481 tagcggctat cgcctaattt gaatcgaact tttccataat cttctatcat ttctatctta
    27541 caagttgaaa gcttaaagta aataaggatt gcgagtcttt tagaaataga tgttttgaat
    27601 taaaagggat aagattttga tggggaaaag aaagtatctt caatcagata cgcttgcatt
    27661 tctgaccgag gcgatttgaa ttatttgaaa tcatcactac attatctgac agtcgtagta
    27721 aattatcgaa tccttttttc tggctttgcc ccagtagttt gcgagaactc tcggctccaa
    27781 aaatgattac ttttttattc attctctttc ctttaatttt ttacttacta aaattccatg
    27841 attagaaaca gtgatgatat gcatttcata gttttctaat atttcaatat acttcattaa
    27901 tctaccatca tctccataac catcagtaac gttatgcatt gtaaacattc cacctattct
    27961 aagttttggt tcacaaagtt taaaaaactt tgtataacca tccgctggtt gtgtgctcca
    28021 agttgcatca gaaaaaacaa aatcaaaatt tgtttttata ctaggaatga ttaacattgc
    28081 atcgcctaaa tgcatttcaa tcagatgaga tactccagca tcttcaaaat tcttttttgc
    28141 tttttcaaat cttcctttat ctatttcaat agtaattact tttccaccag tttgagaagc
    28201 tgcttttgca agccatattg tactatgacc ggatgaggtt cctatttcta aaatattctt
    28261 gtattggttc tttaggatta ttttttcgag cgcccttcca tcctcaaaag gaacgttaaa
    28321 atcactccaa gaacctttct tattttctag ataattttga atttttgtat ccaatgcatc
    28381 cttctttttt aaatcctgtg gctgtaaaaa attgaaactc ataagtaaaa aaataattat
    28441 tttaaccaat tcactatttc ccaaactact ttatcgatcg tgatgcattc ttcatagaat
    28501 tgaaattgaa attgtttttc ttcaaaccaa accaattttt tttctttaga tggaatgagt
    28561 tcaaatattt ttttgggtat atccggtcct gttgctgcat tatcagaatg aatcattagg
    28621 attggagact gtaattgctt aacgataggc gttacatcat gtccccagat atctaattct
    28681 gacatatgtg taattttatt ttcccaagat cctctaaaac caaaataggg agctcgattg
    28741 ttccacggta gataccattc agcgataggt ttaggaaaaa ggagggctgt tgaatctaaa
    28801 tcaaactcac gaacaatagg tatataatca actttgcctg tttcttcaaa cttctcttta
    28861 gagagccttg cctggtttag tttatttttt actccctctt ctccacccac atatctgtga
    28921 gctgtttcgg gagtgagtaa atgagaagca actaaaacta cttttttgat tcttttatct
    28981 aaagtagagg cttctagtgt ccaattcact ccttggcaaa ttcctaaggc aaagatttta
    29041 tcaggactaa tttcttttct tgttagtaag taatcaacag aagactgaac atcttctact
    29101 ttccattttc tattttcaaa tcttctaggt tccccttcac tctctccata agatcgtgga
    29161 tcaaagatga gcgcaatata tccttctttg gctaatcgac tcgcgtattg gattggactt
    29221 tgttcttttg caaatgctac aggtcctaaa atggtaaatg caccttttgg tttttcacta
    29281 ggaatcagta actttccaac taatttcttt cctttactca aaaaggaaac attttctatt
    29341 ttatactctg ccataatttc tcctgaaaat agaagagtga gtagtattaa acttattatc
    29401 tttatgttca ttcccatctt tcctatactc tatttatata taattatagt ctgatcttta
    29461 aaaatcttgt tagaacaaaa aatagaaaga ttggtattat ttgaacttct gatttctaaa
    29521 ttcagaaggg gttaatttgg ttattttttt aaaaaattta gtaaaatagg aaagatcttc
    29581 aaattcaaat tcaaaggcaa tctcagaaat tgatttttct gaatacaaaa gctcagtctt
    29641 tatatctata attttaattt cgtggataat ttcacctgtt gatttgcctg taattttatt
    29701 tacaattcta tttaaatgtt gttgggagat aaataatctt tctgagaaat ctttggatgt
    29761 aaagtagcgt ttaacgtttt ttctaactag ttctacgaat tctgtatatt tctgaatttg
    29821 taaattttca aatacagaaa tatttttttg aaagtagtat tgaatttttt ttagaatgac
    29881 aaagagtaga ctggaaacaa tatcactgtt ttttgctaaa ttgaattcat ttaaaagtag
    29941 attaaaaaag ggagctaaat ctttagagtt ttcttttgtt agatgcatat aaggatttcg
    30001 atataaatga ttcataaata ataattcatt taaaaaattt ttattcccaa tcggacccaa
    30061 aagaaagccc tccgtaaaca taatacaata cccgctaata ttatcataat cttcaaaatg
    30121 atgtaactga tttggtgaga taaaaaataa agtatttggg tttattttat aattctgaaa
    30181 atcaatcgat tgtttacttc taccctttgt tatccaaagg atttcataga aattatgttt
    30241 gtgaggaaat tctatttcag caatctccgg catgtcttca aatctactca cccaaatttc
    30301 agaatcttga tttgtaaatt tctggattcg gtaaattgga aacattgtat tcataggatt
    30361 cttttaggca aattttgtta gactaaatat agctttttct ttctagcggc taacgtctaa
    30421 tttacattca acttctccca taattgtcac ccatttctat ccttcaagtt ataatcttaa
    30481 aacaaaaaag gattacgagt ctttcataaa tattagaatt tttagttaat aatttggcta
    30541 tccctttgat gaaaaaatat aagttataaa aatgattatt atataacggc gatcgcctgc
    30601 gttaagggtt tcgccattgc ttgtcaaaat tgctacattt tttcttctat ggctgctgaa
    30661 tataccactc aatccgctta taatctctgg aaataaaaac tgtgagaatg ccaaattccc
    30721 ctaactctaa atgaaaagga tagaaattag gttgtaaatt gttccaatat ccttcagtgg
    30781 gagctaattg taaaagaagt agccattctt ccgagttgaa tgcttccggt ggagtatcta
    30841 gaccttcgat atataatggt tgaccaccga ttttatttcc tgcaagctca ggtcgagaaa
    30901 actgaaatcc tgtgtcataa tccaagttat cgaacgtgta tcgttcctca agaggaatca
    30961 aatcttcatt ttcttctgct tctaatataa ctctatactc cctataaaca gctcgattat
    31021 cttcctcatc ttcatcttcc tctaattgct gtaattcata tatagctggc ccagtagctt
    31081 ctgaaataaa ctcgatgtca ttaccattcg tgtagacagt ttcaggagtt tgtatcacta
    31141 ctgcaaaagc ttcattataa actggctctg attctgcact aaagaaaata tatactatcg
    31201 tgcccttgct gtcaggaaat aatttttcat caagagcaat ttgccccaga aacagcattt
    31261 gctcacttgt ttcaggagaa agaggccaaa ggggttgacc aatccatact ggttgtcctc
    31321 caaatttagt aaccggttct aaaatttcag taggactttc aacaatttga aatcgagttc
    31381 taagtagcat ttaattaacc tttcatatta acaggttgca gagatatatc tttctttcta
    31441 ggaatttgca ttcaacttct cccataattg tctcccattt ctatcttaca agtgaatggc
    31501 ttaaatcaaa aaaggattgc gaggctttta gaataagatg tttggaactt aacagggtaa
    31561 aatttttatc taggaatttg catcgaactt ctcccataat tgtctcccat ttctatctta
    31621 caagttaaag gcttaaatca aaaaaggttt gtgagtcttt tagaataaga tgttttgaac
    31681 taaacagggt aaaatttgtc aaaggagatt tattacggct aatcgacgtt atccaaataa
    31741 gtcgttccac cgttcgcaac attcgccaaa actttttctg ggcactttgt tataacacaa
    31801 tcggaattcc catcaccggc ataggcttat tagccccttg gatcagcggg gcggcaatgg
    31861 ctattagttc gatttctgtt gtattaaatg cgctgtcgct aaattggagg agggagactt
    31921 aggtcggtag gttcgaatgg gcaaggttag atcgggacgt gtttgtttcg aaatttgaat
    31981 tggattactt ccagacatat ttcccgatat ctagtttttc cttttcggct gagttttgta
    32041 gatttgtgtc agcagttaag aactgtaagt ctttttcaaa ctgcttggag agaattatac
    32101 cagtcgcaag gtgaattgca tcaaaacctc tgagaagata ttttgtatga atccgaatca
    32161 attccgaatt taacgttgta tctacattta ctttgattaa agaatcccaa tcttcgagaa
    32221 aagctttatt gatttggttc tttactctat cagaaaatct ttccattttt tgttttttat
    32281 gaatcgtccc aagaatttca gagtatccaa cttgggaaat cgcaatataa tctgattcgt
    32341 cccagatttg atctatatat cccgaaccaa tttcttggaa gtatttttta attaatacac
    32401 tggaatcgag ataaacgagc attatctgct ctccagtagt aggtcggaag cagatttttt
    32461 acttttcgtt tttaaagaaa cgaatttttt ctttgtccta tttcttgtag gaagttcgat
    32521 cgtacccttt tcagcaagcc tatagagttt ttcggtcagg gaattttttc cagtaggctc
    32581 tttaattaat cgagccacgg cttttccatg ttcggttaca attatctcat ttccagtctt
    32641 tgcttggtgg ataaaatggc ttaattttgc ttttaaatct cttactccaa caagtatcat
    32701 actaatctct ccctgtgact acattttata gaatatggtc acatttctac attgtcaagc
    32761 ttttattttt cttgtagatt ctgctgaatt aaggaattaa atgtttctcc tactatcatt
    32821 caaaaattca ggaacggcaa agagcaagtt agcctcacta cctttttgaa attaaaagat
    32881 tactttggat tggaagtaaa gattttacgg ggcaataagg ctttggcgat aatttgatga
    32941 ccctttataa tcgtttcttt taactttttt caatcctata agtaaattta gaagggtttt
    33001 tggaaatgaa attaatagta ttaagtttca ttacatgaat ctattgcatg gagatgcaaa
    33061 ttttgtttct ggaagaaaac catcaaggct ctttgcttct gggttccagc caattcttgt
    33121 ctgttcagca cataaaaaaa aatagtttga ttaggcttcg atatgaaatt gaccgtaacc
    33181 gctgaattag agcgaaagtt cggcgatgaa aagcggatac gagctttatg atttcctggt
    33241 tttaaaacta caaattcaaa accatacggg aaagattctt catcgtccaa tgaaaggagc
    33301 acaatgtaag gatccaatgt caataacgcc aagtccttag ttggatagag agggcttcca
    33361 ggaaggactc taaattgagt ttcattggga tttgctatat tagataaact tttgctatat
    33421 ttgtaattgt aaccgctgct agcacagttt agaagaaagt aaagaagcaa tagatagggt
    33481 atatttttca tggtatttac gaatgctagt gtttattcga tgtcaattcc tatttagaaa
    33541 aagttaaaga aatatagact tatcagtatt ttagtcatta atcctaatta gattctatta
    33601 aagaaaaaaa cattaaacaa attccaaaca tacatccggg agaaatctta gaagaagagt
    33661 ttttaattcc gttagaaatt acagcatatc gtttagcaaa ggaaacggca ataccacaaa
    33721 ctcgtattag ccagatcata aaaggcaaaa gaagcattac agcggatact gccattcgcc
    33781 tctctaaatt ttctttctag gaatttgcat cgaacttctc ccataattgt ctcccatttc
    33841 tatcttacat gttaaaggct taaagtaaaa aaggattgcg agtctttcat aaaaagatgt
    33901 tttgaactaa acggggtaaa atttttctct ccgatcccgg tcacaagaga atggccgtca
    33961 ccgagaaaaa aaggcacatc cgcgccaata ccggaagcaa attgcaaaaa atcaggattc
    34021 gggttttggg tttcctccgg aaataaaaat ttcagaagac tggcagcgtt tgtgcttccg
    34081 ccgccaagtc ctccgccggg agaaattctt ttagtcagct ttatatgcac gcctggcaga
    34141 ttcggatttc tatcaagaga acgaacgtat gccttgtgta atatattttt acttatattc
    34201 ccctgttcag aaacggcgat ataatcttcc ttttgcctgc cggttaactc aacgattgac
    34261 tcgagagaca tcttttgctc tggcaaggaa ataaattcca tatcgtctcc ccagttcaat
    34321 tttaaaaata ccgaaattaa ttcgtgataa ccgtcaggtc gtttaaaaag aattttcagt
    34381 cccaggttaa ttttagcagg cgacagcatc agcaataatc cttaaaattt cttaacaatt
    34441 cctgaccttt tttattattc ggatcgatat aaagcaattg atccagaatt ttcctggctc
    34501 ttttcgggtt atttgttttg agataagttt tcaccagcaa tagcaagcat cttgtatccg
    34561 ctgttcccct gagcttcaat ctttctaggt aatcagccgc caaatttaat tgcccggatt
    34621 tgaaatacat ataacccgct ttcaaaagca aaacattatt ccagggctcc tgcatggaaa
    34681 gttcttccgt atattttacc gcttcgctcc attttgattg agttctgtaa aaatttatca
    34741 aagcaatcag agagctttta tgatacggat atttttcata aagcaatcta agttccgctt
    34801 cggctttatt cggcattttt tcaaatgtaa attttcttgc cttgccaata tgctcatgaa
    34861 taacggatgt ctccgcttcc ggataagttt ccttattaaa aaattgaatc tttattatgc
    34921 ttaaatcgtc cgaaagttcg cctgttttct tgatcgaatt tataattcga ctcaaatctc
    34981 ccttttccga ttctatgatt tttaaaaata acgtttcatc ttcattaaat aatttcattt
    35041 cggaatcatt acgcaagagt atgtcatctc tgccatccga acctataaaa atcgtatccc
    35101 ccggatagag taaaaatgtt ctaatagtaa tatgattatg caaacctttg attccgattt
    35161 ttaatagagc cgatccttct tccaggaaag aagttttttc atcccgatat aaaaccgtat
    35221 gcggatgctc tgcgttaata taatacatca tcccggtcgt attttctata atgcccatca
    35281 ctacggagat caacatggaa ccatcgaaag attcataagc gttttgcaat tccaaaaacg
    35341 cgttcttgag ccatttttca ggaaatatat tttgattgga acgaacaagt tttgttctcg
    35401 atacgatgga cttaaatatg actcccaaaa ccagagcgcc gccagcgccc tgaattgatt
    35461 ttcccatcgc gtccccgtta ataaaaacag tatatatttt gtcttttaat tcgattacat
    35521 ccgcaataca aatatctccg ccgatctggt attccttgct tttgaaccgg aacttttttt
    35581 tctgttccat aaaaaattcg atcttgcaat cggattttct tgcctcatta tatgcaagag
    35641 gatttacgat aagagacgtc agaaaataat ctccgtcctg ttgcaatttg agtttactca
    35701 cttcatccag cgaacttttt aattcgatag ttcgttcttc gatttttttt tccagcccgc
    35761 tgtttaaatt atttatttcg gaatacatcg aattgagtct tccgataacc tggttcaagt
    35821 gatgagcctg ccttccgaat tcatttgcgg cggaaggcga acacacgata ttaaattgcc
    35881 ctttccccaa ttgggacaaa acatgactga cgtcgtgaat actatttttt attgacttag
    35941 tgcatacata agcggacagg gcaaccggaa tggaaaaaag acaaaccatg attcccaggt
    36001 gaaaaaacgg atcttcaatt ttgatagttc cgaagctgat tccggtcagc atatatccga
    36061 gcacaactaa aggcaagagc atcagcgcaa tcatggaaaa actgattctt tttaatgtgc
    36121 tgaattgcgg aatggaatga aaatctatgt cgatgttttt aaatgtttcc gtctggtaaa
    36181 ccggtcggat cgttatttca ctgatatata aaaaagaaat ataggcaagt ggaagcatta
    36241 aaaccatcag gaaaggatag gtcatgtgca attcggaatg aacttttccc gcgaaaatat
    36301 caaatatgac atgagctaat gctgctgcaa atatccagcg taatgtaatg actatcggtt
    36361 ccatcagagg atattttagt aattttattt ttgactgtct taacaggtta tcaggcaatt
    36421 gaggattctg aatgattccg atttcccttt ttaaaaaaaa ccatctccac ccgattccaa
    36481 tgcacgctcc tatcgttcct gcaatggcgg agccgacaga caagtaaaca aattcatgag
    36541 tttcctttag agttcccgta atcgcagaaa aataaatggc aaccggaaca ttgaccagat
    36601 acgtgaataa ttcgaggcga taggttaatt taaagaaaat ggttttgaac atgaggaact
    36661 ccgaatttca tcttacgaac aaagaatctt ctttttgttt tcctgtcaat aatcagtctg
    36721 ttgcgaaata agaaaaaggg tttacagaat agattttaat aaa
//
LOCUS       DAOQOS010000049        36254 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_475_length_36254_cov_5.737893, whole genome shotgun sequence.
ACCESSION   DAOQOS010000049 DAOQOS010000000
VERSION     DAOQOS010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 36254)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 36254)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36254
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_475_length_36254_cov_5.737893"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            110..1813
                     /locus_tag="PLF66_12325"
     CDS             110..1813
                     /locus_tag="PLF66_12325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000906630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HMZ64874.1"
                     /translation="MLIVIFGAVMVLGISIFIHELGHLLCGMLVGVKARIFSFGYGRG
                     IWKKRIGETTYQITAIPIGGYVMFKGDQYGRRLKGKDGELLSTPPLKRMIPVLGGPLF
                     NLILGFTIFFLIALIGDDNSWGNKIFIDKVNRDYSAAYKAGLRTGDRIVAINGKATET
                     FEDIFTSVGLSGGEEIEVSYERKGETKSIQITPDIYSAGGRPTLGIEPYGERHIVVTF
                     TYKEQIGHWIKSTFDPNKKAFDYFQKNFGQQGVEEISNKKLGKEDESPSTRAIAFLKD
                     GDMILEVEGKPVSTVGELQTLLGQYQNQVVKIHLMRKLIPLISPWAAKEETVEVPVRG
                     SDLLELSQIVDTKNPEFVLQSLVIASHDKNINRKLLNIRIDGRKFLNFAELKTYLNSL
                     STNEIEFESGELHYKCKFRVKPIGLLGFIADMKFEPEAISKKYSVVEALAISGEKVYK
                     SVATSVRALGLLFRGMISVKGNLSGPVGILQSAGISLEYGWLFYFNFVANISIALMFI
                     NLLPIPIADGGHLVLYLYEAIAGKPLPPRAIETLFKIGFIFLLLLGVYVTYYDIMSRN
                     F"
     gene            1819..3558
                     /locus_tag="PLF66_12330"
     CDS             1819..3558
                     /locus_tag="PLF66_12330"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016559869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HMZ64875.1"
                     /translation="MKASNYILPTTKEDPSDAVVASHKLMIRAGLVRKSSSGLYHFLP
                     MGLRVLKKIEKIVREEMDKSGALEFELPILTPSDIWEQSGRWERMGKEMFRINDRHDV
                     PHCLGPTHEESFTSLMKPILKSYKDLPKNVYQIHTKFRDEIRPRFGVIRSREFIMKDA
                     YSFHLDDASLDETYQLMRKTYRQIFNRCGLATIPVQADSGTMGGSGSEEFMVVSPIGE
                     ETLALNDESGYAGNVEKTPVLWKHTAKENQASLEKVSTPNVKAIDEVAKFLGVEPKNT
                     IKAVLVKADEKFDILIFIRGDREVNPVKVNNHFATGEIRPLSNAEIEKLGLVVGFTGP
                     LLTEEAASKLKILFDTSINPTEGYVVGGNEIDFHVKNFVLSRDLKKEVNQKDFCMVEV
                     GDPCPLSGKPLKLEKGIEVGHIFKLGTKYTKAFDVKVSDKSGKMVLTTMGCYGIGVNR
                     TMATIIEQGNDEKGIIWPISVAPFEVCLVSISKSEEEIKKVSEIYEYLNTNGLDIFWD
                     DRDLGPGFKFKDSEIVGFPIRITLGKNFFEKNEFSLLNRKTGEEKNIPFESKEQLLNE
                     VEKLKRELYGALV"
     gene            3755..4954
                     /gene="trpB"
                     /locus_tag="PLF66_12335"
     CDS             3755..4954
                     /gene="trpB"
                     /locus_tag="PLF66_12335"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016548938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HMZ64876.1"
                     /translation="MKEKDFYERKGYFGEFGGRYAPEILTDALLQLEKVYEKLKNNPK
                     FLKELNYYNQNYVGRPSLLTYAERLTKAWGGARIWLKREDLNHTGAHKINNTIGQALL
                     TKSMGKKRIIAETGAGQHGLATATVGALFDMETVIYMGAEDVRRQELNVYKIKLLGAK
                     VVPVTSGTATLKDATSEAMREWALSVANTHYIVGSAIGPHPFPTIVRDFQSVIGKETK
                     QQFKKLNKKLPDAVIACVGGGSNAIGMFYGFINDKKVKLYGVEAGGLGNKPGEHSATI
                     TYGRTGFLHGTKTLIIQDAGGQIVPAHSVSAGLDYPGVGPEHAHLATIKRVEYLSVTD
                     KEALASFVEVSKLEGIIPALETAHAFSAAKKLAKEMGKKKDLVICLSGRGDKDSMEVS
                     RLLGDKL"
     gene            4951..5751
                     /gene="trpA"
                     /locus_tag="PLF66_12340"
     CDS             4951..5751
                     /gene="trpA"
                     /locus_tag="PLF66_12340"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002150802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HMZ64877.1"
                     /translation="MSSLKECFEKDKNRSKFIPYISLGDPEYALSVEWSKALIDGGAD
                     ILELGIPFSDPVADGPVIQKSYMRALAKGFSMDKVLETTKQIHAYKEEIPLVYLTYLN
                     PILSYGEDKFFKKAYLSGIRGIVIPDLPFDAKDAREVIEKARANGIDIIQLITPANTK
                     ERIEALKKFSSGFIYYVTSYGVTGERSNFASDLKKRIEMVRKTMNLPVCAGFGISNAA
                     QAKSIGEYADGIIIGSAIQRIIEEKSANVDLCAKALKDYANEIAKALR"
     gene            5981..8278
                     /locus_tag="PLF66_12345"
     CDS             5981..8278
                     /locus_tag="PLF66_12345"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004779811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HMZ64878.1"
                     /translation="MSEKPSKFSIIDIGAISIFTLGALSVIISAFLPPNVFSAQLSII
                     GVILLLCSFYYSYKFIEKAAKDKQVFGSILIAIALSLTIFTAKNILKLLQTAEESSIS
                     WRFYLLKGTPGKGQIVSEKGYIEKINHIEGARKNIRIIGIKTETLEKLNGTWPIDWRK
                     YAEIINAFQGSDSLLLFDIFFMDYKPAQKEAMEAALKNNKNVIFDYSIESTAESRSSV
                     LNLEKRVNLLRKYQLKNVTDPEDQGISWLSFAVPPIEPISENSAGLGFANIRKEENLP
                     NRHMPLVAKLLRHGPNGETEYFPSIDLIAAAQYYGIDVVSDTEVVMGKYVKLKNIPKK
                     MIKDDKGNEVDIMTNPNPEREITIPIDVYGQMEINFVGSLYCFRDDDLFDVAEWGKGY
                     AENYYDTNTIFLVAMYYATGRGASSDVHLSPYGDMSGIEHHAHALNTILNQSFMASTS
                     EPVDLFIFLIIGILIGFVQPKIRTWLAFIFIFAFVIGYTALSLALFLKSNLIIPLPSI
                     IIEQLMIFVTIIGFKILTEEANVKYIRNTFSKFVSKDVVDELLKHPEKIALGGSRREI
                     TIFFSDIRGFTTLSEALSPEELVKLLNEYLSSMTEIIIETKGTIDKYMGDAIMAFWGA
                     PVDLEDHAYYACIASLIQMDKLEKLQNSWKERNLPSIDIGIGLNTGYAVVGNMGSSQR
                     MDYTCMGDTINLGSRLEGSNKTYGTNIIISEYTYERVKDRVYARELDLVQVKGKTHPV
                     RIYELIGLVNDDDIKKVTKPIVTTN"
     gene            8309..10186
                     /gene="dxs"
                     /locus_tag="PLF66_12350"
     CDS             8309..10186
                     /gene="dxs"
                     /locus_tag="PLF66_12350"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010418102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HMZ64879.1"
                     /translation="MSYKYLDKINDPVDLRKLSREELREVCSEVRTYIIDTLASVGGH
                     FASNLGVVELTVALHYVFNTPIDRLIWDVGHQTYPHKILTGRRESLKTVRKYGGISGF
                     PKREESPYDLYNTGHAGTSISQLLGEAVSRDLLKKKYNCIAVIGDASIATGMALEALN
                     HGGHIRPDCLVVLNDNYMSISKNVGSISNYLNNIITSNFFNKYKKVYYTILKWLPLIG
                     PAMVSVSRRLEKGFKDFLVPGALFEDLGFHYFGPIDGHDIDKLVTVLEKMKNLEGPLL
                     LHVITQKGKGYIPAENDPIKYHGVTPFNIEDGAMNVDSKIGYSKIIGECLIQLTTKNP
                     AVVAITPAMIEGSGLKNYSIKFPQNTFDVGIAEQHSVAFAGALLGGGAIPYMCIYSTF
                     LTRAMDQMVEDISLMNLPVRFVIDRAGCVGSDGETHQGLFDLGYLCGLPNMSIIAPSS
                     AQDLIDTMFYMENYNKGPIAIRFPKASEAADNINYTQRNQIEEGKLRLVAEGKDVTLI
                     SIGSMLDTAKKTKAILEKSNISVGIIDLFWIRPLDVEGLTNIISKSKQFAILDESYID
                     AGASGYVLNRIPPKYLNRFIKTFAFPPDIIIHGEKPEIFKHYRIDADSIASEIISFLK
                     N"
     gene            10231..10698
                     /locus_tag="PLF66_12355"
     CDS             10231..10698
                     /locus_tag="PLF66_12355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64880.1"
                     /translation="MEKINFVSAHNETGKDLFNQGDIERATFYFLKAMEEGDSAESLF
                     YLGLIQNQNGNFRDALSYYYRAILLNPDYGNPCNEIGVILLRHGREREAVFWLKRSVR
                     CSFNDAPHIPLYNLATLYKMWNRPERSLQYLHKALELAPNFLEAIELRNQLLS"
     gene            11298..12251
                     /gene="cas6"
                     /locus_tag="PLF66_12360"
     CDS             11298..12251
                     /gene="cas6"
                     /locus_tag="PLF66_12360"
                     /inference="COORDINATES: protein motif:HMM:NF021551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR system precrRNA processing
                     endoribonuclease RAMP protein Cas6"
                     /protein_id="HMZ64881.1"
                     /translation="MLKSKLNFSKVRVFFEALEDCKLPRFSGSFLRGTFGHALKEAFC
                     VMAHRNCAKCMVRDSCGYFQVFESQDETKRQDGFLYKPHPYILTPCLKNEFKKGEILE
                     FSITIFGEAIRYMPYFIFSFQKMGEKGFGVERYKFNLTQIKDFYTGNSVYDGSLISSR
                     NITELTIQEFASKLCPNPTQENLNYILSFETPARFVENKKDVHILSPSILLDSMIRRY
                     KTMHQFYGEFIDSDLELGNLQLSPYKKQRYISWKRYSNRQGRTLDQGGISGSYQVSLT
                     EPKLALFLYCMEILHVGKSTTFGLGKVGIESLQQDIAMIGG"
     gene            12255..13250
                     /gene="cmr1"
                     /locus_tag="PLF66_12365"
     CDS             12255..13250
                     /gene="cmr1"
                     /locus_tag="PLF66_12365"
                     /inference="COORDINATES: protein motif:HMM:TIGR01894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III-B CRISPR module RAMP protein Cmr1"
                     /protein_id="HMZ64882.1"
                     /translation="MEKITFECEVITPMFLGGVDGKSAELRAPSIKGALRFWWRAMNG
                     DLPLSELHQKEAEIFGGGGDGGRRSQVVVRVEQGNLNKVSLVYQGKIASQPSFYTRPI
                     NAPILKYLSIGTFEFPGNNPRESIKENQNFRIIIQVMKKFETEIIKSLLYLSTFGGIG
                     SKARNGFGSFKVKSINSRPVDYIQIKKNDFKNIIPKYCSFSQNIRLFKTSQSFSKWDK
                     ALEDIGDCYRLARLESDGTKYKFSNRVLLTEPLNQANDQRIIDRRTKPFFMNVRKEQN
                     NYTGYLLYLPSDYIEDTKMLKKQLSKEQINKQYLEACNIFLKKLKTISKITEVVI"
     gene            13247..14980
                     /gene="cas10"
                     /locus_tag="PLF66_12370"
     CDS             13247..14980
                     /gene="cas10"
                     /locus_tag="PLF66_12370"
                     /inference="COORDINATES: protein motif:HMM:TIGR02577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III-B CRISPR-associated protein Cas10/Cmr2"
                     /protein_id="HMZ64883.1"
                     /translation="MNNYLFLFTIGPVQSFIAQARKTKDLFAGSQILSDLIEEAINAL
                     IKMEPQSEIIFPGKGIPSKPNRFIAIIDNPEMIGKNLESIIRNSFREKVLKDIKDDFK
                     NINDEKLNFHANSFLEIYWNAIPIENNSYNAHEYTRLEKELGEFKNARIFNQWEINSE
                     KNRKCSICGERDNLVIKEKQLPNTISIKNDFRFEEGEGLCGICLYKRIYKVSSDSQSF
                     DSTAEIATMDSIEYLLKNEKDIFLSYKNQIQSQFFDSQLLFEENLNRDYFKKQKKEPL
                     IPNLNQLQKELKEINKKLEKAKLKFQKYYAIIVFDGDNMGKWLSGNNNLLKEDVPLKD
                     FHKSLSECLGNFAKDCKDFLKNPNGRSVYAGGDDFLGFINLSYLIKSLNYFYESFDKL
                     INLPLKSYLKENQKLSFSAGIAVAHYKTPLSEVINSARVMEKEAKSIDITKDAFAITI
                     LKHSGERQSCKLHWNDLKDSSNISKIEEIVQSLQTEKFSNTFMQNIVQEFKKLISKDK
                     NESLNKIVTAEIDRLVERAYLLKEDKKENSEKLSKVVKDLYLYNDLNSKNPNNFFSVL
                     GIADFLRKHKN"
     gene            15002..16069
                     /gene="cmr3"
                     /locus_tag="PLF66_12375"
     CDS             15002..16069
                     /gene="cmr3"
                     /locus_tag="PLF66_12375"
                     /inference="COORDINATES: protein motif:HMM:TIGR01888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III-B CRISPR module-associated protein
                     Cmr3"
                     /protein_id="HMZ64884.1"
                     /translation="MTTIKINPLDVLFFRDGKPFSMGEDVWATGIFPPFPSTLYGALR
                     ASYFSDHIDKLEYANIEGKDDSLKLEIKKVYYSENSNVLFPIPLDLVRKVSTKKDEES
                     ALLLKLKENSSSISNYPFKKVLSLDINEKLESLSGNYFYNFELQKYLKKESEKFSYID
                     SQKLLIQESKIGIARNKFTKTSDEGKIYRVSMIRSKENIDFGIHIEYENLELESKGKL
                     RLGADGKIASFEKVENQRIEPPKINNLFKLYLSTPAIFTNGWLPFGMNPEKLETQKDG
                     IHLKLLTCAIGRYTNVGGFQIKKGIQSSKAMYKAVPAGSVYYFETDSNEPNKIIELFH
                     GKSISDIHSKEGFGICFVGAV"
     gene            16071..17321
                     /locus_tag="PLF66_12380"
     CDS             16071..17321
                     /locus_tag="PLF66_12380"
                     /inference="COORDINATES: protein motif:HMM:NF021189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64885.1"
                     /translation="MKKIITTVGTSIITNCLQEDDSFISILGSVKDSFSDEWDSQKSN
                     IEIIRKKVTSWANIKGEKASAEIQSILKIKEELNEEVEVYLISTDTVSSRLSAEIIKN
                     FFYEFNANITVIFNFKEDVIKGLQVINKSKFEKEGLVELIKRIEEITENNWEDSVFNI
                     TGGYKALIPFSTIFCQFNRIRAYYLFQEDEKSKEKFELISFPVIPINLDYNFFEKNIN
                     NLKLLNKGVDNFYELKKTIPKELYPFIWSEEDLAELNSIGKYFFNKYESHLIIYMLKG
                     MASTIPEQKYSKRLYDSFDSLYSQLEKYLISNSNLINANTNELKKCLDEQGECALNHG
                     PLKNSEYYIFKAPMTKPEIRLCYNFEISNGIIVKIRVFDFRIGEFDHSKYFNEFSISY
                     KHHKEKNNLNELIPITYLKGINNY"
     gene            17358..18257
                     /gene="cmr4"
                     /locus_tag="PLF66_12385"
     CDS             17358..18257
                     /gene="cmr4"
                     /locus_tag="PLF66_12385"
                     /inference="COORDINATES: protein motif:HMM:TIGR02580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III-B CRISPR module RAMP protein Cmr4"
                     /protein_id="HMZ64886.1"
                     /translation="MFKLAKPLFLICETPLHAGSGDSLGVVDLPIQRERHTGFPKIEA
                     SSLKGALRESFEVKIEDRKNKNLFSDVYSLFGPEDDDAKLNPRSGSLAFTDARLLFFP
                     VKSVKGVYAYVTCPLILEKFIKELKLSIPEMKDIPNIQFSESKRTKTSSLIVSGDKII
                     LEEYTYEVKVDDNLSTFAKWISEKLFNTPEQKYWKDKLTNDLVVLSNDDFKDIVNLFT
                     EVITRTKINNETGTVETGALFTEEFLPQESILYSLVMASNEFIENGKKANEMMSNFES
                     GLQSVFQLGGNSTLGKGLLRRVF"
     gene            18259..18636
                     /gene="cmr5"
                     /locus_tag="PLF66_12390"
     CDS             18259..18636
                     /gene="cmr5"
                     /locus_tag="PLF66_12390"
                     /inference="COORDINATES: protein motif:HMM:TIGR01881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III-B CRISPR module-associated protein
                     Cmr5"
                     /protein_id="HMZ64887.1"
                     /translation="MPVINKIEQGRAEYAYKCAEKGKGIGKEYKSNVKKLPMLIKTNG
                     LGASLAFFFSKGKEETKPHGLINKQIHEWLKQNNPDLFSPQSELIKTIVDLDSVKYRF
                     ITNEVLSFLNWLKRFSDGLIKED"
     gene            18637..19920
                     /gene="cmr6"
                     /locus_tag="PLF66_12395"
     CDS             18637..19920
                     /gene="cmr6"
                     /locus_tag="PLF66_12395"
                     /inference="COORDINATES: protein motif:HMM:NF015728.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III-B CRISPR module RAMP protein Cmr6"
                     /protein_id="HMZ64888.1"
                     /translation="MNEFEGVIVENFFIKFKNQNNEEKSLRIPGDIYGLTKGQFVGEK
                     CKITVENGQISKVILLSTGKELPKKQLAEDNRPNKNGEHKHKERHPDNRSEITIPQNN
                     KSDKFYGPIDTNELFKQFAGIENYLLKLQKFATAENPGTNKEKFTFYKKDSKEIKPNY
                     NSSTINSLSIRHISNAKSLLGAKNIKDITLKTDWRMALGLGNESVYETSITLHHIYGI
                     PYIPSSSIKGIVRSWIITNCFSDNGNNMLEAEGRAIENQLFCEIFGCPSELKVEEKDK
                     KKTFKSYYQSNEPKIGGDREGKIIFFDAYPINLINESIKVDIMNPHYSDYYSSEGNNI
                     KPPGDYYNPVPIPFLTVQNTSFQFIIGTKQIPKKIIEYKEYEKLKPFDESSLKSEERF
                     KDSFTLIDLAYLWLKLALAEHGIGAKTAVGYGYMK"
     gene            19917..20600
                     /locus_tag="PLF66_12400"
     CDS             19917..20600
                     /locus_tag="PLF66_12400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64889.1"
                     /translation="MKKKYSTIILLTFLLISFTCKYAVKAELYISEILETANKSSKES
                     FAVSELKFGVGSLEKCEEDKEYLTNLLSNYFNNIRDLKCANIEMDTFLISTVDIPIVA
                     FTKKEFEPVDGLLNITVKPGKPIRVGLFLNESEFSDLKAKINDKYWQSIKMEDFSLNF
                     TLFNDTKQITKLEVTSSFVNSEPVPFWQSYQLNPKNKLSIELSNVLKEYINKKTISDF
                     IKIEVESKK"
     gene            20608..21006
                     /locus_tag="PLF66_12405"
     CDS             20608..21006
                     /locus_tag="PLF66_12405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64890.1"
                     /translation="MFLNVTAHELTTEQRELAASYSDTIVDLKKLNPSLHERLVSCPL
                     EKGELLALADELLMFIGEVSQMNKNRLTLHLPIGSPAFMALFFMKLDREKLPCRIVFS
                     HSDRVSVDEKQEDGSVVKRAVFKFVKFIEI"
     gene            21035..22027
                     /locus_tag="PLF66_12410"
     CDS             21035..22027
                     /locus_tag="PLF66_12410"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HMZ64891.1"
                     /translation="MQSKDVEHLEQIKFEQMETIFQIIGMIESKSALLEKSSKPMTRE
                     KMEEYLNQIEGTPKIKRKNKEDSYPDKRRQIANVRKIFNRLSNKFHIEGNRLGTSFIN
                     LRTGSNNQKGLKEDNAYQTLLVIYLSLLLYKGTIHLDFLYQFLELEFPFDFLTSFQYS
                     IHKKINISFHYRSDRNNHDFIVDPFLPVKIYFKDNHWFLVGWDLKYNSWNQYLIHSIK
                     KLTLSSKKLDLSKIPNFDFIEYRKSAFAGGVLNNSPIHTVEIEVPLDLYEAVRKRRRE
                     GNWEKKENFYIWKLQTYDLNEIFDYIFRWNGRLKIKSPDSVKKEFQTKIRSFLE"
     gene            complement(22125..23351)
                     /locus_tag="PLF66_12415"
     CDS             complement(22125..23351)
                     /locus_tag="PLF66_12415"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HMZ64892.1"
                     /translation="MVKLTPNKINFKIKDSFDFDSFLSALNWDLKNESVEIDFSESKL
                     VDYQALALFVPYLMYLKNQNCKLEIKIIKDSVLGKMWDKLDATKWDKITAKNDVNFSF
                     HKTKPFLAIRSYEDVKRVIHRIENYTKNFHIEYEKTLRYIISELLYNTIEHGHNENIP
                     SFVQFSNNVKVSEISFVIADTGVGIKEHLKKAYPGIESDIDAIKLAIKPQVSGTFIPN
                     SPYQNGNNAGMGLFISTNIVKKLYSDIYIISGHGQVHISPTDITEKRLENSWHGTLVY
                     VNLRFGKTTKVNLEQVMHSLREEAEQEIKNRDSQVTTGHYLNIKNYFGGYAEDKGEAI
                     NYRDKYLVANAKDDKDLILDFADVVNSPHSFLNALLSIPIKIYGMKAYKKIKVKNAQP
                     NIRETIDFILDDLTRE"
     gene            23523..23771
                     /locus_tag="PLF66_12420"
     CDS             23523..23771
                     /locus_tag="PLF66_12420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64893.1"
                     /translation="MKKIDNPNVLDLVLAFISNHRNPIEINVSIRNKDGSREIQIFTG
                     KKETIVTFTFKILLLFVMSGLYILSDGNIIHTNQNKSH"
     gene            23791..26220
                     /locus_tag="PLF66_12425"
     CDS             23791..26220
                     /locus_tag="PLF66_12425"
                     /inference="COORDINATES: protein motif:HMM:NF016955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HMZ64894.1"
                     /translation="MIKLVLIIFIILVIAIFRIVRGIVKTVSNELPFWTEAAGKILNE
                     FLEEENDSGHNPTANLKYRPSFETAFKRQMDQFARFIDQNCKTNLQTFQFLEFCISMH
                     MAKADGVIDSSEIDGIRNYFKETYVEHLDETLLAKASPLVREYIQNYEPDELILSACL
                     TLNVWYDILEDTFPDSEIRDELTMQIFSFVYQVAMMDGVIASSEERFFKGVCEFFKFP
                     TDFQDHIKRTAEYQFNVRKNKANTENKEAKDFENALKLFQLTPNYTKEDLQKAWKNFA
                     KLNHPDRFHNVDRELYEKINAQFLDAKNGYDLLLSKIENPTAKPYYEPSPSAAPSQEE
                     QKQEPPKEEKKETYYSYSKEEFREPSEKVYTEPEKNPFLAYLGNLVSNIQNFVVTKFS
                     PAQLKLIFWGNTKRKMVTIATPIVILLASILIPLLVESHYKKLLESDRVSDYNNAYRY
                     FKVQGEASKDLMKELFRNSNNSILKTNTLHYLLEEKENSSDTHEFILEMLKKEDPIVI
                     KNLEHLNGYYPWSDSIYNSTLELLKRKNEHLREVVAKMIQPRLKQLPIQNKNLEIFLG
                     CIKDTSYTVSKFCWNGLEHYKEEDKVQKAILENLDSISPEIKDITHSMLWELLLREEG
                     TDKLIEANKKYPLTAFILSSISSEENKVLTIFEKNPKFRKANYRAWLGILNKKSEDDI
                     KQYLSSLDESVRREPSIRPLILKYEKEVETKMPEPDLVEPDSYLSEREENHNPDNSIR
                     YARVNEPSGLFLRDKCSTNSSKIELIPNGERVEVLSKDREDYLYDVRSNWYKIRYQGK
                     TGCSFGAFLSF"
     gene            26221..27231
                     /gene="cas1"
                     /locus_tag="PLF66_12430"
     CDS             26221..27231
                     /gene="cas1"
                     /locus_tag="PLF66_12430"
                     /inference="COORDINATES: protein motif:HMM:TIGR00287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas1"
                     /protein_id="HMZ64895.1"
                     /translation="MADLYITEHGISLHRSGESLLLKKDGTQFKELELKNIETIQIFG
                     YAQASSQVLFELLERGIELAFYNSSGNLIGQLTPPHVKNIELRMKQYDLSKNESFKLD
                     FGKEMIQGKMESCIYLLEEFGKNSEFDFRSEILALKDLKSASSLSQNIESLLGFEGIF
                     AKRYYENYGKLFREKGLFNGRSRRPPKDAANSVLSLLYTILTNRISAYIDGIGFDPYI
                     GFLHSLEYGRLSLGCDMIEPLRPLFCDWFTLKLFNQKRIKPEDFRVEDNGYYLDDLSR
                     KKFYPLLHNELKENRDYLYLRGELLDWVKMQTEWLKTVFLAGSVTSFKEYAKAKQNNG
                     TG"
     gene            27218..27520
                     /gene="cas2"
                     /locus_tag="PLF66_12435"
     CDS             27218..27520
                     /gene="cas2"
                     /locus_tag="PLF66_12435"
                     /inference="COORDINATES: protein motif:HMM:TIGR01573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HMZ64896.1"
                     /translation="MEQGKLYLVCYDFPSSKAGDRRRVKVVKILEGYGNRVQKSVFEL
                     RIHKKEELDQLLKKIKRWFIEAEDSLRIYPLNAIAESEIQIHGEGEIYKVEDAYFF"
     repeat_region   27817..30014
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=27817..27853
                     /rpt_unit_seq="ctaacggatgagtttcctcgaatcagaggattgcgac"
     gene            31093..31875
                     /locus_tag="PLF66_12440"
     CDS             31093..31875
                     /locus_tag="PLF66_12440"
                     /inference="COORDINATES: protein motif:HMM:NF023043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="HMZ64897.1"
                     /translation="MAVEQSEISSKSRLITLLLSFLFVHRFYVGRIVSGIFFFFTAGG
                     FGFWWLIDVIQILSGSFKDQFGKFILNWEADKKQLTLSGVALGFVVLLAMLGGKNKNG
                     ETSSPTFKDALQSSGSNSTEEKKSFPTVGDKIQTSHFAITLKGYKIQNSVKTGNMFSK
                     LDPEEGNKYLVLDVNYKNTDNESRMIGGEGKVFVEMNGKEFEYDKSEHILLDGWGVLL
                     DQLNPMTQKNTKLVFKIPDELKGKIYWEPVRSSDRFFVGEAK"
     gene            32145..32516
                     /locus_tag="PLF66_12445"
     CDS             32145..32516
                     /locus_tag="PLF66_12445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64898.1"
                     /translation="MTPSDLPYRERYAELYNRSKEGGIRKVESAEEFIFKLNSTETDF
                     LYIPAKHTIQVYLAMKGLGFTSNFEKLYELTRQEIGEEFANMFHYLFSLNEKQFDATK
                     EECILGLEYAKKILALYSSEV"
     gene            32586..32753
                     /locus_tag="PLF66_12450"
     CDS             32586..32753
                     /locus_tag="PLF66_12450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64899.1"
                     /translation="MEYFPARKTRKAGYAVEVFNLIGETIGVYDLSESQLEPIPQSAV
                     PSIRKIQKIAV"
     gene            32890..33195
                     /locus_tag="PLF66_12455"
     CDS             32890..33195
                     /locus_tag="PLF66_12455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64900.1"
                     /translation="MLSAEKIKLLQELTKETNPDASIEKIVQDYIDTKIAYWRMVDKF
                     YSEKMGMSFEVYEDTKREEWDGSDWNKIEEFHDWEDAFIGIKYYLELKDKWIFQTSN"
     gene            33171..33365
                     /locus_tag="PLF66_12460"
     CDS             33171..33365
                     /locus_tag="PLF66_12460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64901.1"
                     /translation="MDISDIQLKLESKLPQIDSIEDIHFYQVDEYSIKGNATVKKIYN
                     LNIRFKTFHERMTLSFALIY"
     gene            complement(33711..34400)
                     /locus_tag="PLF66_12465"
     CDS             complement(33711..34400)
                     /locus_tag="PLF66_12465"
                     /inference="COORDINATES: protein motif:HMM:NF026198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding domain-containing protein"
                     /protein_id="HMZ64902.1"
                     /translation="MIKYSLIKNRLSTAGVSPYLPKTLLEGSLVFEQFLKALAYGSTA
                     TLADVKAVLENLERVCIENLSQGRSVNLGFCTIRPQVKGSFQNLEDSFSQEKNWIEVS
                     IAPNPNFLKKITQDAKLQRVSLSKTLPLLFSLENHTTGLEASLVAGDLVTIHGENLKF
                     DKSVPNVGVFFEGAGVEQRVSEYSKIAGKSISFKVPNGLNPGVSYKLKVKNSFGQEIR
                     TGELEMEIKAA"
     gene            34645..34968
                     /locus_tag="PLF66_12470"
     CDS             34645..34968
                     /locus_tag="PLF66_12470"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HMZ64903.1"
                     /translation="MENPKQFYKAIGRRIADARQSMHMSQEDAADKLNITRVTWNHIE
                     KGNQKLTLDRLIDIAGLLQIAPTKLVPGLVSSDETDVLTEKNFSSDEQALILERLDKY
                     KKQKK"
     gene            35020..35538
                     /locus_tag="PLF66_12475"
     CDS             35020..35538
                     /locus_tag="PLF66_12475"
                     /inference="COORDINATES: protein motif:HMM:NF017885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HMZ64904.1"
                     /translation="MESKFANEIKKLFTLIQYKTGAVDLNKIAEKLNIRVIEDDLPDN
                     VSGVLDCRTKDDPIILLNSNHHLNRQRFSLAHEIGHFVLHKMSGVHMDTKIFLRSDAP
                     NLHGVRIEREANQFAAELLMPEEEIRKAWKNLPEDWFDKSPLEVIAKQFKVSEGALFI
                     RLKQLKGIGGIW"
     gene            35582..35980
                     /locus_tag="PLF66_12480"
     CDS             35582..35980
                     /locus_tag="PLF66_12480"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HMZ64905.1"
                     /translation="MIQLPNPSYETAIPIFQKFNDLQAVYLFGSVATGQTNAESDVDF
                     GFVADKNIKDELSLELIKAGFGNFSLVYIPEATPVLQFEIVDKNFILYQKPDFDTSSF
                     RSKIIKVYLDMEYYKGIQRQYLKERILNGK"
     gene            35970..>36254
                     /locus_tag="PLF66_12485"
     CDS             35970..>36254
                     /locus_tag="PLF66_12485"
                     /inference="COORDINATES: protein motif:HMM:NF014043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HMZ64906.1"
                     /translation="MENKSVIQTKIELLNDYLSFLQQSQKYSESEFIQSMMIQGSVER
                     YLHLSIETTIDMGNHIISKNGWGSVHWYQDIATLFQEKGYIDSELAALWIR"
BASE COUNT        12694 a         5499 c         6801 g        11260 t
ORIGIN      
        1 tctgttttgc aatctatacc aattgaatat cctgaagcat tggaaccatt tctagaagcg
       61 gatcgaaagg caagagaatg ggttcgggaa aattcatagg aggtcttaat tgttaatcgt
      121 tatttttgga gccgtaatgg ttctcggaat ttctatcttt atccatgagt tgggtcattt
      181 actgtgtgga atgttagtcg gtgtaaaggc tcgtattttt tcatttggtt atggcagagg
      241 catttggaaa aagagaattg gagaaacaac ctatcaaatc actgcaattc caatcggtgg
      301 atacgttatg ttcaaaggag atcaatacgg aaggcgctta aaaggaaaag atggtgaact
      361 tctttctact cctccactca aaagaatgat tccagttcta ggtggacctc tatttaattt
      421 gattttaggt tttacgattt tctttttaat tgctctgata ggagacgata actcttgggg
      481 caataagatt tttattgata aggtaaatcg ggattattcg gcagcctata aagcagggct
      541 tcgaacaggg gatcgaattg ttgcaatcaa tggaaaggcg acagaaacct ttgaagatat
      601 ttttacaagt gttgggcttt cgggtggaga ggaaattgaa gtttcctatg aaagaaaagg
      661 ggagacaaag tcgattcaaa ttactcccga tatttattcc gcaggaggaa gacccacttt
      721 aggtattgag ccctatggag agcggcatat tgttgttacc ttcacttaca aagaacaaat
      781 aggacattgg ataaagtcta cttttgatcc aaataaaaaa gcgtttgatt attttcaaaa
      841 aaactttggt caacagggag ttgaagaaat ttccaataag aaactaggaa aagaagatga
      901 atctccttct acgagagcaa tcgcattttt aaaagacgga gatatgattc tagaagtaga
      961 aggtaagcca gtttctactg ttggagagtt gcaaactctt ctaggacaat atcaaaacca
     1021 agtagtgaaa attcatctaa tgcgaaaact gattccactg atttctcctt gggcagcaaa
     1081 ggaagaaaca gttgaggttc cagtgagagg ttcagaccta ttagaactat cgcaaatagt
     1141 agatacgaaa aatcctgaat ttgttttaca aagtctagtg atcgcaagcc acgataaaaa
     1201 tattaatcga aaactattaa atattcgaat cgatggaagg aagtttttaa atttcgcaga
     1261 attaaaaacc tacttaaatt cattgtctac aaacgagata gaatttgaat ccggagaact
     1321 tcattataaa tgtaaattta gagtaaaacc aattggtcta cttggattta tagctgatat
     1381 gaaatttgaa ccagaggcaa tcagtaaaaa gtattctgtg gtagaagcgt tagctatttc
     1441 aggagaaaaa gtttataaat cggttgcgac atcggttcga gcactcggac ttttatttcg
     1501 tgggatgatt tccgtgaaag gaaatttatc tggacctgtt ggaattttac aatctgctgg
     1561 aatcagcttg gaatatggat ggttatttta ttttaacttt gtggcaaata tttctattgc
     1621 acttatgttt ataaatcttt tgcctattcc aattgcagat ggcggacatc ttgttttata
     1681 tctctatgag gctattgcgg gtaagccgct accacctaga gcgattgaaa ctctatttaa
     1741 aatcggattt atatttttac tattattagg agtgtatgta acatactacg atatcatgtc
     1801 gagaaatttc taaaaactat gaaagcatct aattatattt taccaaccac aaaagaagat
     1861 ccatccgatg cagtcgttgc atctcataaa ttaatgatcc gtgcggggct tgttcgaaag
     1921 tcctcttctg ggctttatca tttcttacca atgggtctta gagttttgaa aaagattgaa
     1981 aaaattgttc gagaagaaat ggacaagagt ggagcactcg aatttgaact accaattctt
     2041 acgccttctg atatttggga acaaagtgga agatgggaga gaatgggaaa agaaatgttt
     2101 agaataaacg ataggcatga cgtgcctcat tgtctgggtc ctacacatga agaatctttt
     2161 acttctctta tgaaaccaat tttaaagtcg tataaagatc tacctaagaa cgtttatcag
     2221 attcatacaa aatttagaga tgaaatcaga cctcgcttcg gagtgattcg ttctcgcgag
     2281 tttattatga aagatgcata ttcttttcat ctagatgatg cttccttaga tgaaacctat
     2341 cagttgatga gaaagacata tagacagatt tttaatcgtt gtggtctagc aacgattcct
     2401 gttcaagcag attcagggac tatgggggga agtggatcag aagaatttat ggttgtttcg
     2461 cctatcggag aagagacact tgcactcaat gatgaaagtg gatatgcagg aaatgtcgaa
     2521 aagactcccg ttttatggaa acatactgcg aaggaaaatc aagcgagtct agagaaagta
     2581 tctactccga atgtgaaagc gatagacgaa gtagcaaagt ttttaggtgt agaacctaaa
     2641 aatacaatta aggcagttct cgtaaaagcg gatgagaaat ttgatatatt gatatttata
     2701 cgaggagata gagaagtaaa tccagttaag gtaaacaatc attttgcgac aggagagatt
     2761 agacctttgt ccaatgcaga gattgaaaaa cttggcttag tagttggatt tacaggacct
     2821 cttcttacag aggaagcggc ttctaaactt aaaattttat ttgataccag tatcaatcca
     2881 acagaaggat atgtagtggg aggaaatgaa attgatttcc atgttaaaaa ttttgtttta
     2941 tctcgtgatc taaaaaagga agtaaatcaa aaagattttt gtatggtgga agtaggagat
     3001 ccttgtcctt tatcgggaaa acctctcaag ctagaaaaag gaatcgaagt aggtcatatt
     3061 tttaaattgg gaacaaaata tacaaaagca ttcgatgtaa aagtttctga caagtcaggc
     3121 aaaatggtat taaccacaat gggttgttat ggaattggag tgaatcgaac catggcaaca
     3181 atcatagagc aaggaaacga tgagaaagga attatctggc caatatcagt tgctcctttt
     3241 gaagtttgtt tggtgagtat ttctaaatca gaagaagaaa taaaaaaagt ttcagaaatc
     3301 tatgagtatt taaacacaaa tggtttagat attttttggg acgatagaga cttaggtccc
     3361 ggttttaagt tcaaagattc agagattgtt ggatttccaa tacgaattac tcttggtaaa
     3421 aatttctttg agaaaaatga attctcatta ctcaatcgaa agacaggaga agaaaaaaac
     3481 attccatttg aatcaaaaga acaattatta aatgaagtgg aaaaattgaa aagagaatta
     3541 tacggagcat tagtttaatc ctcttagggc taagctgacg atttacggat taccactgat
     3601 gcacaccgat aacgaaacga taaagaaacg atggatatcc tatgaacatc acatctttta
     3661 tcggtgttca tcgtcttcat cggtggtaat ctttgaacaa gccccctttt tcgcaaaggg
     3721 ggcagggggg atttgagagt cgaggagttt atttatgaaa gaaaaagatt tttatgaaag
     3781 aaaaggttat tttggagagt ttgggggacg atatgcgccc gagattttga cagatgcact
     3841 tctacaacta gagaaagtgt atgaaaaatt aaagaacaat ccaaaatttc taaaagaatt
     3901 aaactactac aatcaaaatt atgtaggtag accgtctctc cttacgtatg cagaaagact
     3961 taccaaagca tgggggggag ctagaatttg gctcaagaga gaagatctaa accatacagg
     4021 tgctcataag attaataaca caatcggaca agctcttcta acaaaatcca tgggcaaaaa
     4081 aagaatcatt gcggaaacag gagctgggca acatggatta gccactgcga cggtaggcgc
     4141 actattcgat atggaaacag tcatctacat gggtgctgag gatgtcagaa gacaagagct
     4201 caatgtttat aaaataaaac ttctaggagc aaaagttgtt ccagttactt caggaactgc
     4261 gactctaaaa gatgcaacaa gcgaagcaat gagagaatgg gcacttagtg ttgccaatac
     4321 tcattatatt gttggatctg ctataggtcc gcatcctttt ccgactatcg ttcgagattt
     4381 tcaatccgtg atcggaaaag aaacaaaaca acaatttaag aaattaaata aaaaattacc
     4441 agatgctgtg attgcctgtg tgggcggagg ctccaatgcg attggaatgt tttatggatt
     4501 tataaatgat aaaaaagtta aactttatgg agtagaagcg ggtggacttg gaaacaaacc
     4561 aggggaacat tctgctacta tcacttatgg tagaactgga tttttgcatg gaactaaaac
     4621 tttaatcata caagatgcag gtggacagat tgttccagct cattctgtct cggcaggact
     4681 agattatcct ggagtaggac cggaacatgc gcatcttgcc acaatcaaga gagttgaata
     4741 tcttagtgta acagataaag aagcgttagc ttcctttgta gaagtttcaa aattagaagg
     4801 aattattcca gcactcgaga cagcacacgc tttttctgct gctaaaaagt tagcaaagga
     4861 aatgggaaag aaaaaagatt tagtaatttg tttatcagga agaggagaca aggattccat
     4921 ggaagtaagt cgtttactag gagataagct atgagcagtt taaaagagtg ttttgaaaaa
     4981 gataaaaatc gctctaagtt tataccttat atttctttgg gagatccgga atacgcgctt
     5041 tctgtagaat ggtcgaaagc actcattgat gggggagcgg atattttaga attaggaatt
     5101 ccattttcgg atccagttgc agacggacca gtgattcaga agtcttatat gagagctctt
     5161 gcaaaaggtt tttcaatgga taaggtttta gaaactacaa aacaaatcca tgcatataaa
     5221 gaagagattc cactcgttta tcttacttat ttaaatccaa ttctatctta cggcgaagat
     5281 aaatttttca agaaggcata tctatcagga attcgtggca ttgtaattcc agatttacca
     5341 tttgatgcaa aagatgcgag agaagtgata gagaaagcaa gagcgaatgg gattgatatc
     5401 attcaactca tcacacccgc caacacgaaa gaaagaattg aggcactaaa gaagttttct
     5461 tccggcttta tttattatgt gacttcttat ggtgttacag gagagagaag taattttgca
     5521 tcggatctaa aaaaaaggat agagatggtt cgaaaaacaa tgaacctacc cgtttgtgct
     5581 ggatttggaa tttccaatgc agcccaagct aagtctatcg gagaatatgc agatggaatt
     5641 attataggat cagcaattca aagaatcatt gaggaaaagt cagccaatgt ggatttatgt
     5701 gcaaaagcac taaaagacta tgcaaatgaa atagcaaaag cacttcgttg attggggttt
     5761 ttaaatactc atttggagtc attattaatt cggagcaaag gaattttaga atgaaaagaa
     5821 tactgagtct ttcgttaggt aaaatgtttc ttagcgtgga ggattcagtt gagaagataa
     5881 gtcattaatc tctgtttaaa taacttaatt cttgacgatt ctcctttcat tttgaatgat
     5941 tcaaaaagtc ttacatacaa aatttagttc ggagcaatct atgagcgaaa agccaagcaa
     6001 gttttctata atagatattg gagcaatatc tatttttaca ttgggcgcac tatctgtcat
     6061 tatcagtgca tttcttccgc caaatgtatt ttccgctcaa ttgagcataa taggtgttat
     6121 cctacttctt tgctcttttt attattcata taaatttatt gagaaggcag ccaaggataa
     6181 acaggttttt ggtagtatac tcattgcgat tgctctatcc ttgactattt ttactgcaaa
     6241 aaatattcta aaattactgc aaacagcaga agagtcttct atctcatgga gattctattt
     6301 actcaaaggc actccaggga aggggcaaat cgtttccgaa aaaggttata ttgaaaaaat
     6361 taatcatatt gaaggtgcta gaaaaaatat ccgcatcatt gggattaaga cagagacttt
     6421 agaaaaacta aatggaactt ggccgattga ttggagaaag tatgctgaga ttatcaatgc
     6481 attccaaggc tcagacagtt tactcctttt cgatattttc tttatggatt ataagccagc
     6541 tcaaaaagag gcaatggaag cagcccttaa aaataataaa aatgttattt ttgactattc
     6601 gattgaatct actgctgagt ctagaagttc tgttttaaac ttagaaaaac gagtcaattt
     6661 acttcgcaaa taccaattaa aaaatgttac tgatccagaa gaccaaggga tttcttggct
     6721 tagttttgct gttccaccga ttgaaccaat ctctgaaaac tctgcgggtc ttggatttgc
     6781 aaatattcga aaagaagaaa atcttccgaa ccgtcatatg ccacttgtgg caaaactatt
     6841 aagacacgga cccaatggag aaacagaata cttcccgtct atcgatttaa ttgccgctgc
     6901 tcagtattat ggaattgacg ttgtgagtga tacagaagtg gtcatgggca aatatgttaa
     6961 actaaagaac attcctaaaa aaatgatcaa agacgataaa ggcaacgaag ttgatataat
     7021 gacgaatcca aacccggaga gagaaattac aattcctata gatgtatacg gacagatgga
     7081 aattaatttt gtgggttctc tttattgttt tagagatgat gacttatttg atgtagcaga
     7141 gtggggcaaa ggctatgccg aaaactacta tgatacaaat acaatcttct tagtagcaat
     7201 gtattacgca acaggaagag gggcatcgtc tgacgttcac ttatctccat acggggatat
     7261 gtcagggatt gaacatcacg ctcatgcttt aaatacaatt ttaaatcaga gctttatggc
     7321 gagcacttcc gaacctgttg atttatttat atttttaatt attgggattt taataggatt
     7381 tgtgcaacct aagataagaa cttggctcgc ttttattttt atatttgcct ttgtgattgg
     7441 ctatactgca ctttctctcg cattgttttt gaaatctaat ttaattattc ctcttccgag
     7501 tataattatt gaacaattaa tgatctttgt gactatcatt ggatttaaga ttttgacaga
     7561 ggaagcaaac gttaaataca tacgaaatac tttctcaaaa ttcgtatcta aagatgtggt
     7621 agatgaactt ttaaaacatc cagaaaaaat tgcacttggg ggttctagga gagagatcac
     7681 aattttcttt tctgatatta gaggatttac aacactatca gaggcattaa gcccagaaga
     7741 attggtgaaa ttgttaaatg aatatttatc tagtatgaca gagattatta ttgagacaaa
     7801 aggaacgata gataaataca tgggtgatgc gattatggct ttctggggag caccggtaga
     7861 tttagaagat catgcttatt atgcttgtat tgcatctttg atccaaatgg ataaactaga
     7921 aaaattgcag aatagttgga aagagagaaa tctaccgtct atcgacatag ggattggact
     7981 caatacagga tatgccgttg taggaaatat gggaagttct cagagaatgg attatacctg
     8041 tatgggtgac acaatcaatc ttggttcaag gttagagggt tcaaataaaa cttatggaac
     8101 aaacatcatc atttctgaat atacatatga gcgtgtaaaa gatagagtct atgcaagaga
     8161 gttagattta gttcaggtga aaggaaaaac acatcctgtt cgaatttacg aactcatagg
     8221 actagtaaat gatgatgata ttaaaaaggt gactaaacca attgtgacaa caaattaatt
     8281 ccaaagtatc ggaaggtttt cgagtagtat gagttataaa tatttagaca agataaacga
     8341 tccagtagat ttacgaaaac tttcccgaga ggaattgaga gaagtttgtt cggaagtaag
     8401 aacctacatc atagatacac tcgcaagtgt aggcggacat tttgcaagca acttaggagt
     8461 tgtagaacta acggttgctt tgcattatgt atttaacacc cccatagata gacttatttg
     8521 ggatgtagga catcaaacct atccacataa gattttaacg ggaagaagag aatctctaaa
     8581 aacagttcga aaatacgggg gcatctcagg atttcccaaa agagaagaat ctccctatga
     8641 tctttataac acaggtcatg caggaacttc tatttctcaa ctgctaggag aagctgtttc
     8701 tagagattta ttaaaaaaga aatacaactg cattgcagtg ataggcgatg cgtcaattgc
     8761 tacaggtatg gcgcttgaag ccttaaatca tggaggtcat atccgacctg attgcctagt
     8821 ggtgctaaac gacaactata tgtctatttc gaagaatgtg ggatctattt cgaattatct
     8881 aaataatatc atcacgtcta atttttttaa taaatacaaa aaagtatatt acacaatttt
     8941 aaaatggcta ccactcattg gtcctgctat ggtgagtgta tctagaagat tagaaaaagg
     9001 gtttaaggat tttttagttc caggtgcact ctttgaagat ttaggatttc attattttgg
     9061 tccgattgat ggacatgata ttgataaatt agtaacagtg cttgaaaaaa tgaaaaacct
     9121 agaaggtcct cttcttttgc atgtgatcac acaaaaagga aaaggttata taccggcaga
     9181 gaatgatcca atcaaatacc atggagtgac tccgtttaat atagaagatg gtgcaatgaa
     9241 tgtagattcc aagattggtt atagcaaaat cataggagaa tgtctcattc aactaacgac
     9301 caagaatccg gcagtcgttg cgattacccc tgcgatgata gagggaagtg gattaaaaaa
     9361 ttattcgatc aaatttcccc aaaatacatt tgatgtagga attgcagaac agcattctgt
     9421 tgcgtttgca ggagcgttac tcggtggggg agccatccct tatatgtgta tctattctac
     9481 ctttttaaca agagcaatgg atcaaatggt agaagatatt tctcttatga atcttccagt
     9541 gcgctttgta atcgatagag caggctgtgt gggttcagat ggagaaacac accaaggtct
     9601 ttttgattta ggttatcttt gcggtctacc taatatgagt atcatcgcac cttcaagtgc
     9661 acaagattta attgatacta tgttttatat ggaaaattat aacaaaggtc caattgcaat
     9721 tcgatttcca aaagcaagtg aagcagcgga taatatcaat tatacacaga gaaaccaaat
     9781 agaagagggc aaacttcgat tagtcgcaga aggaaaagat gtaactctga tatcaattgg
     9841 gtctatgtta gacactgcta agaagacaaa agctatatta gaaaaatcta atatttcggt
     9901 tggaatcatt gatttatttt ggattcgtcc actcgatgtg gaagggttaa caaatataat
     9961 ttctaaatca aaacagtttg ctatattaga tgaaagttat atagatgcgg gtgcgtcggg
    10021 ttatgtctta aatcgaattc ctccaaagta tttaaatcgt tttattaaga catttgcatt
    10081 tccacctgac ataataattc atggtgagaa accagaaata tttaaacact atcgcattga
    10141 tgcagattca atcgcctctg aaattatttc ctttctgaaa aattaaagac ctgtttttct
    10201 aagaccgatt atatagtaac ggttaagtaa atggaaaaaa taaattttgt atctgctcat
    10261 aatgaaacag gaaaagacct attcaatcaa ggcgacatag aaagagccac tttttatttt
    10321 ctaaaagcaa tggaagaagg agatagtgcg gaaagccttt tctatttggg tctcattcag
    10381 aatcagaatg gaaattttag agatgcactt tcttattatt atagagcgat cctgttaaat
    10441 ccagattacg gaaatccgtg caatgaaatt ggtgtgattc tactcagaca tgggagagaa
    10501 agagaagcag tattttggtt aaagcgatcg gttcgctgct ctttcaatga tgcacctcat
    10561 attcctcttt ataatctagc aactctctat aaaatgtgga atcgtcccga aagatctctt
    10621 caatatcttc acaaagccct agagctagcg cctaacttcc tagaagcaat cgaattaaga
    10681 aaccagttat tgtcataggt tgagattccc gtgtaggtga tacaggggtg tcttacctaa
    10741 tgctgtaaac ttaggaatta ccaccgagga caccgataac tgaacgatga agaaacgatt
    10801 gagaattttt aattatcgta tcttttattg gtgccatgcg gtgtggattt gacaagctca
    10861 ctaaccaagc ctagtccgaa ccgttcatcg tctttcctct caaaatccat tgccgctcgt
    10921 tctcggtaat aggtggtaat ctctgagaat ttcccattga ctgtattgtg tctcacttac
    10981 acgggacgga gtcgtttatt cgttatatct acacaagcgt gcttgatctt ttgtttgaaa
    11041 tattagataa tcaactgaaa gattcgataa aattccagca ttttagtaaa gcacaaatat
    11101 atatgtaagt tttccaaaga ctctatttaa taaagtgctc tctaaaaaaa ttgaatataa
    11161 tataaaaata attctagaga ataaaaaaga attctattga atttcgagag ggatgtcgat
    11221 tcttgataca tctctataaa cggaaggatt tactagggat aaatactaat tgtttagatt
    11281 agtaaagaga ggaagttttg ttaaagtcaa aattaaactt ctcgaaggta agagtttttt
    11341 ttgaagcact agaggattgt aaattacctc gttttagcgg ctctttcctg agaggaacat
    11401 ttggtcatgc cttaaaagag gctttttgtg taatggcgca tcgaaattgc gccaagtgta
    11461 tggtaagaga ctcctgcgga tactttcaag tatttgaaag ccaagatgaa actaaaagac
    11521 aagatggatt cttgtataag ccgcatccgt atatcttgac tccttgttta aaaaatgaat
    11581 ttaaaaaagg ggaaatatta gaattttcca ttacaatttt tggggaagca attcggtata
    11641 tgccttattt tattttctcc ttccaaaaaa tgggagaaaa aggttttgga gtagaaaggt
    11701 ataaatttaa tctcactcaa ataaaggatt tttacacagg taattctgtt tatgatggta
    11761 gtcttatttc ttctcgaaac atcacagaat taacgatcca ggaatttgca tctaagcttt
    11821 gtccaaaccc aacacaggag aatttaaact atatcctttc ctttgaaact cctgctcgat
    11881 ttgtagaaaa taaaaaagat gttcatatcc tctctccttc tattctatta gatagcatga
    11941 ttcgtcgtta caaaacgatg catcaattct atggtgagtt tatcgattca gatctagaat
    12001 taggcaacct acaactgtct ccttacaaaa aacaacgtta tatttcatgg aaacgatatt
    12061 ccaaccgtca aggcagaacc ctcgaccaag gcggaatctc tggttcctat caagtctccc
    12121 tcacagaacc taaactagca ctttttcttt attgtatgga aatcctacat gtaggtaaga
    12181 gcacaacgtt cggtctaggc aaagtaggga ttgaaagttt acaacaagac attgcaatga
    12241 ttggaggatg aaaggtggaa aaaataactt ttgaatgtga agtaattact cctatgtttt
    12301 tagggggcgt agacggaaag agtgcagaat tgcgtgctcc tagtattaaa ggagcactgc
    12361 gtttttggtg gagagcgatg aatggagatt tgccgctttc agagctccat caaaaggagg
    12421 cagaaatttt tggaggtgga ggagatggtg gaagaaggag tcaagtggtg gtaagggtgg
    12481 agcagggaaa cttgaataaa gttagtttgg tttatcaagg taaaattgca agtcaaccta
    12541 gtttttacac tcgacctata aatgcaccta ttcttaaata tttatcaatc gggacctttg
    12601 aatttcctgg aaataatcca agagaatcaa taaaggaaaa tcaaaacttt aggataatta
    12661 ttcaggttat gaagaaattt gaaactgaaa taattaaatc cctcttatat ctatccactt
    12721 ttggtggaat tggttctaaa gcaagaaatg ggtttggttc atttaaggtg aaaagtatta
    12781 attcaagacc agtagattat attcaaataa agaaaaatga ttttaagaat attattccaa
    12841 agtattgttc tttttctcaa aatattcgtt tatttaaaac ttctcaatca tttagtaaat
    12901 gggataaggc gctcgaagat ataggagatt gttatcgatt agcaagattg gaaagtgatg
    12961 gtactaaata taaattcagc aatcgagttt tacttacaga acctttaaat caagcaaacg
    13021 atcaacgtat aattgataga agaactaagc cattctttat gaatgttaga aaagagcaaa
    13081 ataattatac aggatatctt ttatatcttc catcagatta tattgaagat actaaaatgc
    13141 ttaaaaagca attatctaaa gagcaaatta ataagcaata tttagaggca tgcaatatct
    13201 ttttaaaaaa gttaaaaaca atttctaaaa tcacagaggt tgtaatatga ataattatct
    13261 attccttttc accattggtc ctgttcaatc ttttattgct caggcacgta aaacaaagga
    13321 tttatttgca ggcagtcaaa ttttaagtga tttgatagaa gaagctatta atgcattaat
    13381 aaaaatggaa cctcaatctg aaattatttt tccaggaaaa ggaataccat ctaaaccaaa
    13441 tcggtttatt gcgattatag ataatcctga aatgatagga aaaaatttag aatcgatcat
    13501 tagaaatagt tttcgcgaaa aagttttaaa agacataaaa gatgatttta aaaatataaa
    13561 tgatgaaaaa ttaaattttc atgctaacag ttttttggag atttattgga acgctattcc
    13621 aatagaaaat aattcttata acgcccatga atatacgcgt ttagaaaaag agctgggaga
    13681 attcaaaaat gcaaggattt ttaatcagtg ggaaataaat tcagagaaaa atcgaaaatg
    13741 ctctatttgt ggagagagag ataatcttgt tataaaagaa aaacagttac cgaatacaat
    13801 ttctattaaa aatgatttta ggtttgagga aggagaaggc ttatgcggta tttgcctgta
    13861 taaaagaata tataaggttt cttcagattc tcaaagtttt gattccacag cggaaattgc
    13921 aacaatggat tccatagaat acttactaaa aaatgaaaaa gatatttttt tatcgtacaa
    13981 aaatcaaatt caatctcagt tttttgatag tcaattatta tttgaagaaa atttaaatcg
    14041 ggattatttc aaaaagcaga aaaaagaacc attgatacca aatcttaatc agctacaaaa
    14101 ggaactaaaa gaaataaata aaaaactaga aaaagcaaaa ttaaaatttc agaaatatta
    14161 tgccataatt gtttttgatg gtgataatat gggtaagtgg ttatctggaa ataataacct
    14221 attaaaagaa gacgtacctc ttaaagattt tcataagtct ttatccgaat gcctcggaaa
    14281 ttttgcaaaa gattgtaaag attttttgaa aaatcccaat ggaagatcag tttatgctgg
    14341 aggtgatgac tttctaggat ttattaatct ttcttattta ataaaaagtc ttaattattt
    14401 ttatgaaagt tttgataaat taattaatct accactgaaa tcttatttaa aagaaaatca
    14461 aaaacttagc ttttcagcag gaatagcagt ggcacattat aaaactcctc tctctgaagt
    14521 tataaattct gctagggtta tggaaaaaga agctaaaagt atagatatta ctaaagatgc
    14581 atttgcaatc acaattttaa agcattctgg agagagacaa agttgtaagt tacattggaa
    14641 tgatttaaag gatagttcta atatttcaaa aattgaagaa attgttcaaa gtctccaaac
    14701 agaaaaattt tcaaatactt ttatgcagaa tatagttcaa gaatttaaga aattaatttc
    14761 aaaagataaa aatgagtcct taaataaaat tgttaccgct gaaattgata gattagttga
    14821 aagagcttat ttactaaaag aagacaaaaa ggaaaattct gaaaaattat caaaagtcgt
    14881 aaaggatttg tatttatata acgatttaaa ctcaaaaaat cctaataatt ttttttcagt
    14941 tttaggaatt gcagactttt taagaaagca taaaaattaa aataaaaatg agaaataaat
    15001 tatgactact attaaaataa atcccttaga tgtattattt tttcgagatg gaaaaccgtt
    15061 ttccatggga gaggatgttt gggcaacagg tatcttccct ccatttcctt ctaccttata
    15121 cggtgcatta agagcttcct atttttcaga tcatattgat aaattagaat atgctaatat
    15181 agagggaaaa gatgattctt taaaattaga aatcaaaaaa gtatattata gtgaaaactc
    15241 taatgtatta tttccaattc ctttagattt agttcgaaaa gtaagcacaa aaaaagatga
    15301 ggaatctgct ctactattaa aattaaagga aaattcgagc agcatttcta attatccatt
    15361 taaaaaagtt ttatcattgg atattaatga aaaattggag tctctatcag ggaattattt
    15421 ttataatttt gaactacaaa aatatttaaa aaaagaatca gaaaaatttt cttatataga
    15481 ttctcaaaaa ctcttaatcc aagaatcaaa aattggaata gcaaggaata agtttactaa
    15541 aacctcagat gaaggtaaga tatatagagt ttcaatgata agaagtaaag aaaatattga
    15601 ttttggaatt catattgagt atgagaattt agaattagaa agcaaaggaa aattaagatt
    15661 aggtgcggat ggaaagattg ctagctttga gaaagtagaa aatcagagaa ttgaaccacc
    15721 aaaaattaat aacttattta agctttattt atctactcca gccatcttta caaatggttg
    15781 gctaccattt ggaatgaatc ctgaaaaact tgagactcag aaagatggaa ttcacctaaa
    15841 attattaaca tgtgctattg gaaggtatac taacgttgga gggtttcaaa ttaaaaaagg
    15901 gattcaaagc tctaaggcaa tgtataaagc cgtccctgca ggaagtgtct attattttga
    15961 aacagattct aatgaaccaa ataagatcat tgagttgttt catggaaaat caatatcaga
    16021 tattcattcg aaagaaggat ttggaatttg ttttgtaggg gctgtatagt atgaaaaaaa
    16081 ttataacaac tgtcggtaca tcaataataa caaactgtct tcaagaagat gatagcttta
    16141 ttagcatttt gggatcagtt aaagattctt tttccgatga atgggattct caaaagtcaa
    16201 atatagaaat aataagaaaa aaggttacaa gttgggctaa tattaaaggt gaaaaagctt
    16261 ctgcagaaat tcaaagtatt ttgaaaatta aagaagaatt aaatgaggaa gttgaagtat
    16321 atttaatttc tactgataca gtatcatcaa gactatcagc tgaaattatt aagaattttt
    16381 tttatgaatt caatgctaat ataactgtaa tttttaactt taaagaagat gttataaaag
    16441 gtttacaggt tatcaataaa agtaaatttg aaaaggaggg tttagttgag ttaataaaaa
    16501 gaatagagga aattactgaa aataactggg aggattcagt cttcaatatt actggaggtt
    16561 ataaagcatt aattcctttt tctacaatat tttgtcaatt taatagaata agagcttatt
    16621 acctgtttca ggaagatgaa aagtctaagg aaaagttcga attgataagt tttcctgtaa
    16681 ttcctatcaa tttagattat aatttttttg agaaaaatat taataatcta aaattattaa
    16741 acaagggagt tgataacttt tacgaattaa aaaaaacaat tcctaaagag ctttatcctt
    16801 ttatttggag tgaagaagat ttagctgaat taaactctat tggtaaatat ttttttaata
    16861 aatatgaaag tcatttaata atttatatgt tgaaaggaat ggcttctaca atacctgagc
    16921 aaaagtattc taaaaggtta tatgattcat ttgactctct ctattctcaa ttagaaaaat
    16981 acttaatttc gaatagtaac ttaataaatg caaatacaaa tgaattaaaa aaatgccttg
    17041 atgaacaggg agaatgcgct ctaaatcatg gtcctttaaa aaattctgaa tattatattt
    17101 ttaaagcgcc gatgaccaaa ccagaaattc ggttatgtta taattttgaa atttctaatg
    17161 ggattattgt gaaaatcaga gtttttgatt ttcgaattgg tgaatttgat cactcaaaat
    17221 attttaatga gtttagtatt tcgtataaac accataaaga aaaaaataat ttaaatgaat
    17281 taattcctat tacctatttg aaaggaatca ataattatta atttgtatta ataaaataaa
    17341 taaatagagg acataaaatg tttaaacttg caaaaccatt gtttttaatt tgtgaaaccc
    17401 cactccatgc aggaagtgga gattcattag gagttgtaga cttaccaata caaagggaac
    17461 gtcatacagg atttccaaag atcgaagctt ccagtttaaa aggagcctta agggaaagtt
    17521 ttgaagtaaa gattgaagat agaaaaaata aaaatctttt ttcagatgtg tattctttat
    17581 ttggtcctga agatgatgac gctaaattaa acccaaggtc cggttccctt gcttttacag
    17641 atgcaagatt acttttcttt cctgttaagt cagtaaaggg agtgtatgct tacgtgacat
    17701 gtcctcttat tttagaaaaa ttcatcaaag aattaaaatt atccattcct gaaatgaaag
    17761 acattcctaa tattcaattt tcagaaagta agagaacaaa gactagttca ttaattgtat
    17821 ccggtgataa aattatttta gaagagtata cttatgaagt aaaagtagat gacaaccttt
    17881 caacttttgc aaaatggatt tctgaaaaac tttttaatac cccagaacaa aaatattgga
    17941 aagataaatt aacaaatgac cttgttgttt tatcaaatga tgattttaag gatattgtaa
    18001 atttatttac agaagttata actaggacaa aaatcaataa tgaaacaggt actgttgaaa
    18061 ccggagcctt gtttactgaa gagtttttac cacaggaaag tattttatac tcattggtga
    18121 tggcatcaaa tgagtttatc gaaaatggca aaaaggcaaa tgagatgatg agtaattttg
    18181 aaagtggact acaatcagtt tttcaattag gtggaaattc cacattaggg aagggattat
    18241 taaggagggt tttttaatat gccagtgatc aataagatag aacagggaag ggctgagtat
    18301 gcatataaat gtgcagagaa aggaaaaggc atcggaaaag aatacaaatc taatgtaaaa
    18361 aaacttccaa tgctaataaa aacaaatgga ttaggggctt ctttagcttt ctttttttca
    18421 aagggcaagg aagagacaaa gccgcatggg ttgattaata aacagattca tgaatggtta
    18481 aaacaaaata atcctgattt attttctcct caaagtgaat taattaagac aattgttgat
    18541 ttggattctg taaaatatcg attcattacc aatgaagtcc tctctttttt aaattggtta
    18601 aaaaggtttt ctgatggatt gattaaggag gattaaatga atgaatttga aggtgtgatt
    18661 gttgaaaatt tttttattaa atttaaaaac cagaataatg aagaaaaatc tttaagaata
    18721 ccaggcgata tttatggttt aacaaaaggt caatttgttg gcgagaagtg taaaataacg
    18781 gttgagaatg gtcaaatttc aaaggtaatt ttattatcca ccggaaagga actacccaaa
    18841 aaacaattag cagaagataa tagaccgaat aaaaatggtg aacataagca taaagaaagg
    18901 catcctgata atagaagtga aataacaatt cctcaaaata ataaatctga taaattctat
    18961 ggtccaattg atacaaatga actatttaaa caatttgcag gtattgaaaa ttatttattg
    19021 aaattgcaaa aatttgcaac cgcagaaaat ccaggtacga ataaagaaaa atttacgttt
    19081 tataaaaaag attcaaaaga aataaaacca aactataatt cctcaactat taattccctc
    19141 tcaatccgac atatatcaaa cgcaaaatca ctattaggcg ctaagaacat aaaagatata
    19201 actttaaaaa cagattggcg catggctcta ggtctaggca acgaatctgt ttacgaaact
    19261 tccattacat tgcatcatat atatggcatc ccttatatac cgtccagttc cataaaaggt
    19321 attgtaagga gttggattat aactaactgt ttttctgata atggaaataa tatgttggaa
    19381 gcagaaggaa gggcaataga gaatcaattg ttttgtgaaa tatttggatg cccttcggaa
    19441 ttgaaagtcg aagagaagga taagaaaaag acctttaaaa gttactatca atcaaatgaa
    19501 ccaaaaattg ggggagatag agaagggaaa ataatattct ttgatgcgta tcctataaat
    19561 ttaattaatg aaagtataaa agtggatata atgaatccac attactcaga ctattactct
    19621 tcagaaggaa ataatattaa acctcccggt gattattata acccggttcc gattcctttt
    19681 ttaacagttc agaatacttc ctttcaattt ataatcggaa caaaacagat tccaaagaaa
    19741 atcattgaat ataaagaata tgaaaaatta aaaccgttcg acgaatccag tttaaaatcg
    19801 gaagagcgtt ttaaagattc atttactcta atcgatttag cgtatctatg gctcaagctc
    19861 gctctcgccg aacacggcat tggtgcaaag acagcagttg gttatggtta catgaaatga
    19921 aaaaaaaata ttcaacaatt atactactaa cgtttttact tatctcattt acttgtaagt
    19981 atgctgtaaa ggcagaatta tatatctcgg aaattctaga gactgcaaat aagagttcta
    20041 aagaaagttt cgctgtatcg gagctgaaat ttggtgtagg gtctttggaa aaatgtgagg
    20101 aagataaaga atacttaacg aatctccttt ctaattattt caataacatt agagacttaa
    20161 agtgtgcgaa tattgaaatg gatacttttt taatatctac agttgatatt cccatagtag
    20221 catttacaaa aaaggaattt gagccagtag atggattatt aaatattact gttaaaccag
    20281 gtaagccaat tcgagttgga ctttttttga atgaatctga attttccgat ttaaaagcaa
    20341 aaatcaatga taagtattgg caaagtatta agatggagga tttttcatta aacttcacct
    20401 tatttaatga tacgaagcaa attaccaaat tggaagtaac aagttccttt gtaaattcag
    20461 aacccgttcc tttctggcaa tcctatcaat tgaatccaaa gaataaactt agtattgaat
    20521 tatcaaatgt attaaaagaa tatataaaca aaaaaactat ttctgatttt ataaagattg
    20581 aagtagaaag taaaaaataa ctcatatatg ttcctaaacg tcacagcaca tgaactaaca
    20641 acagagcaac gcgagttagc cgcaagttat tctgatacga ttgtggattt aaagaaatta
    20701 aatccaagtc tacacgagag attggtttcg tgtccgctgg agaaggggga gttattggcg
    20761 ttagccgatg agttattaat gtttatagga gaagtgtcac aaatgaataa aaatagacta
    20821 acgcttcatt tgcctatagg gagtcctgct tttatggcgc ttttttttat gaagttggat
    20881 cgagagaaac ttccttgtag aattgtattt tcacattcag atcgagtgag tgtagatgaa
    20941 aagcaagaag atggaagcgt tgtaaaaaga gcagtgttta agtttgtgaa atttatagaa
    21001 atatagacaa gcgatagaaa gccgatagaa aactatgcaa tcaaaagacg ttgaacattt
    21061 agaacaaata aaatttgaac aaatggaaac tatctttcaa attataggaa tgatagaatc
    21121 taaatcagca ctcttagaaa aaagttctaa gcctatgacg cgagaaaaga tggaagagta
    21181 tctaaatcaa atcgagggga ctccaaagat aaaaagaaaa aataaggagg atagctatcc
    21241 agataaacga aggcagattg cgaatgttcg taaaatattc aatcgcctca gtaataagtt
    21301 tcatatagaa ggaaatcgtc taggcacttc ctttataaat ctaagaactg gttctaataa
    21361 tcagaaggga ttaaaagaag acaatgccta tcaaacatta cttgtaatct atttgtctct
    21421 acttctatac aagggaacga ttcatttgga ttttttgtat caatttttag aattggaatt
    21481 tccatttgat tttcttacat cctttcaata ttcgattcat aaaaaaataa atatctcttt
    21541 ccactatcgc agtgatagaa ataatcatga ctttattgta gatccatttc ttcctgtgaa
    21601 gatttatttc aaagacaatc attggttttt agtaggttgg gatttaaaat acaattcttg
    21661 gaatcaatat ttgattcata gtattaaaaa acttacattg agttctaaaa aattagattt
    21721 atctaaaata ccaaattttg attttataga atatcgcaaa tctgcctttg caggtggagt
    21781 cttaaacaat agccctatac ataccgttga aattgaagtt ccgcttgatt tatacgaagc
    21841 agttcggaaa cgaagaagag aagggaattg ggaaaaaaaa gagaactttt atatttggaa
    21901 gcttcaaacc tatgatctaa atgaaatctt tgattatatc ttccgttgga atggtcgttt
    21961 aaaaatcaaa tctcccgatt ctgtaaaaaa agaatttcaa acaaagatta gaagcttttt
    22021 agaataatgt taacgtcagt ttggtgtaac agaaaattta aaagatgacc accgataaag
    22081 acgatgggca ccgataggtc ttttagttag attttgttat ttaattattc tctcgttaga
    22141 tcatccagaa taaaatctat tgtttctcta atattcggtt gtgcattttt aacttttatt
    22201 tttttgtaag ctttcatccc atatatctta attggaatgg ataataatgc attcaaaaag
    22261 ctgtgcggtg aatttacgac atccgcaaaa tctaaaatta aatccttatc atcttttgca
    22321 ttggctacta ggtatttatc tctataatta atagcttctc ccttatcttc tgcataaccg
    22381 ccaaaataat ttttaatatt taaataatgt cctgtggtta cttgagagtc tcggtttttt
    22441 atttcttgtt ctgcctcttc tcttagagaa tgcatcactt gctctaaatt gacttttgtt
    22501 gttttaccaa accgcagatt tacatagacc agtgttccat gccaactatt ttcaagtctc
    22561 ttttctgtta tatcggttgg ggaaatatga acttgtccat gacctgagat gatatagata
    22621 tcagaatata atttcttaac tatatttgtg gaaataaata atcccattcc cgcattgttt
    22681 ccattttgat atggactatt cggaataaaa gttcccgaga cttgtggttt aatggctagt
    22741 ttaatagcgt ctatatctga ttcaatgcct ggatatgctt tttttaaatg ttctttaatt
    22801 cctacacccg tatcagcaat tacgaaagat atttcactaa cctttacatt gttgctgaat
    22861 tgaacaaatg aaggtatgtt ctcattgtgt ccatgctcaa tcgtattgta taataattca
    22921 gaaattatat atctaagagt tttctcatat tcaatatgaa agttctttgt ataattttcg
    22981 attctatgaa taacacgttt aacgtcttca taggatcgaa tggctaaaaa aggtttagtt
    23041 ttatgaaaag aaaaatttac atcattcttt gctgtgattt tatcccattt agttgcatcc
    23101 aatttatccc acattttacc caaaacactg tctttaatta ttttaatttc taatttacaa
    23161 ttctgattct ttagatacat gagataagga acaaacaatg cgagtgcttg atagtcaact
    23221 agcttcgatt ctgaaaagtc tatctccacc gattcatttt ttaagtccca atttaaagca
    23281 gatagaaatg aatcaaagtc aaaactatct tttattttaa aattaatctt gttcggtgta
    23341 agctttacca tatctcattc attcagtaat tatagttttt tgtcaaaaga aatatttttg
    23401 tttaaataca cgcgtccgtg tttgtaataa aaaagctatt cgtataaaat atgcgaagag
    23461 cataaatgca tttttaagtt catttaatct ctgactttag gcttaatttt caaggagatt
    23521 ttatgaagaa aatagataat ccaaatgttc tagatttagt cctagctttt atttccaatc
    23581 atcgcaatcc aattgaaatc aatgtaagca ttcgaaataa agatggatct cgagagattc
    23641 aaatttttac cggcaagaaa gaaacgatag ttacatttac ttttaagata ctgcttttat
    23701 ttgtaatgag tggattgtat attttatcgg atggaaatat cattcataca aatcaaaata
    23761 aatctcatta atcttctata aggaaatacc atgatcaaat tagttcttat tatttttatt
    23821 atcttagtca tcgcaatatt tagaattgtg aggggaattg tgaaaacagt ctccaatgaa
    23881 ttgcctttct ggacagaagc cgctggtaaa atattaaatg agtttttaga agaagaaaat
    23941 gactctggtc ataacccgac ggcaaatcta aagtatcgtc cttcttttga aactgctttt
    24001 aaaagacaaa tggatcaatt cgcaaggttc atcgatcaaa attgtaaaac caatctgcaa
    24061 acgtttcaat tcttagagtt ttgtatttct atgcacatgg caaaagcaga cggagtcatt
    24121 gacagttctg aaatagatgg aatccgtaat tattttaaag aaacgtatgt agaacaccta
    24181 gatgagacgt tacttgcaaa agcatctcct cttgtaagag agtatattca aaattacgaa
    24241 ccggatgaat taattctttc tgcttgtctt acgctcaatg tatggtatga tattttagaa
    24301 gatacatttc ctgattcaga aatcagagat gaattgacca tgcaaatatt ttcttttgtc
    24361 tatcaggtgg ctatgatgga tggagtgatt gcttcttccg aagaaagatt ttttaaagga
    24421 gtctgtgaat tctttaaatt cccaaccgat tttcaagatc atatcaaaag aacagcagaa
    24481 taccaattca atgttcgtaa aaataaagcg aatactgaga ataaagaagc aaaggatttt
    24541 gaaaatgctc tgaaactttt tcaacttact ccgaattaca caaaagaaga tctacagaag
    24601 gcatggaaga attttgcaaa attaaaccat cctgatcgat ttcataacgt tgatagagaa
    24661 ctctatgaaa aaataaatgc tcaattctta gatgcgaaaa atggctatga tctacttctt
    24721 tcaaaaatag aaaatccaac tgcaaagccg tattatgagc cttctccttc tgccgctccc
    24781 tctcaggaag aacaaaaaca agagcctcca aaagaagaga aaaaagaaac ctattattcg
    24841 tatagcaaag aagaatttag agaaccttcc gaaaaagttt atacagaacc agagaagaat
    24901 ccattccttg catatttagg aaatcttgtt tcgaatattc aaaattttgt tgttactaaa
    24961 ttttcgcctg ctcaattgaa actgattttt tggggaaaca cgaaaagaaa gatggttaca
    25021 attgcaacac ctattgtcat ccttcttgca agtattttaa ttccactgct agtagaatcc
    25081 cattacaaaa aattattaga atcagatcgt gtatccgatt acaataacgc ttatcgttat
    25141 ttcaaagttc agggagaagc ttcaaaggat ttaatgaaag aactatttag aaattcaaac
    25201 aattcaattt taaaaacgaa cactcttcat tatttattag aagaaaaaga gaatagttca
    25261 gatacacatg aatttatctt agagatgttg aaaaaagaag atccaattgt aattaaaaat
    25321 ttagagcatt taaatggata ttatccttgg agtgattcga tttacaattc tactttggag
    25381 ttattgaaac gaaaaaatga acatctaaga gaagtagtgg caaagatgat tcaaccaaga
    25441 ctgaaacaac ttccgattca aaataaaaat ttagaaattt tcttaggatg tataaaagat
    25501 acaagttaca cagtaagtaa attttgctgg aatggattag agcattataa agaggaagat
    25561 aaggtccaaa aggcaatcct tgaaaatcta gattcaattt ctcctgagat caaagacatc
    25621 actcactcta tgctttggga acttctttta agagaagaag gaacagataa gttaatagag
    25681 gcaaataaga agtatccgct cacagcattt attttaagtt ccatttcaag cgaggaaaat
    25741 aaagtgctta ctatttttga aaaaaatcct aagtttcgaa aagcaaatta tagagcatgg
    25801 cttggaattt taaataaaaa atcggaagat gatatcaaac aatatctttc ttctttagat
    25861 gaaagtgtaa gaagagagcc ttctatacga cctctgatcc ttaaatatga aaaggaagta
    25921 gaaactaaaa tgccagagcc agatttagtg gaaccagact cgtatctaag tgaaagagaa
    25981 gaaaatcaca atcccgataa ttccattcga tatgcaagag tcaatgaacc ttccggttta
    26041 tttcttcgag ataaatgttc taccaattct tctaaaattg aattgattcc aaatggagag
    26101 agagtggaag ttctttcaaa agatagagaa gactatttgt atgacgttag aagcaattgg
    26161 tataaaatac gctaccaagg aaaaactggt tgtagctttg gagcattttt gtctttttaa
    26221 atggctgatc tatatataac agaacacggt atcagtctac atagatcggg cgagtctctt
    26281 cttttgaaaa aagatgggac gcagttcaaa gagttagaac ttaaaaacat agaaaccatt
    26341 cagatttttg gatatgcgca ggcttcttcg caagtcttgt tcgaactatt agagagagga
    26401 attgaacttg ctttttacaa ttcaagtgga aatttaattg gacaactaac gcctcctcat
    26461 gtaaaaaata ttgaactcag aatgaagcaa tatgatcttt ctaagaatga aagttttaaa
    26521 ttagattttg gaaaagaaat gattcaaggg aaaatggaaa gctgtattta tttgctagaa
    26581 gaatttggaa agaactctga atttgatttt agaagcgaaa ttctagcttt aaaagattta
    26641 aaaagtgctt cttccctttc gcagaatata gaatctcttc ttgggtttga aggtattttt
    26701 gcaaagagat actatgaaaa ttatggaaaa ctttttagag aaaaaggatt attcaatgga
    26761 agaagtagac gtcctccgaa agatgcggct aattctgttt tgtctctact ctatacaatt
    26821 ttaacaaatc gaatttctgc ttatatagat ggaattggat ttgaccctta tattggtttt
    26881 ctacatagcc ttgaatatgg aagactgagt ttaggttgtg atatgattga accacttcgt
    26941 cctttatttt gtgattggtt tactttgaaa ttattcaatc agaaaagaat caagcctgaa
    27001 gattttagag tagaggataa cggctattat ctagatgatt tgagtcgaaa gaaattctat
    27061 ccacttcttc acaatgaatt aaaagaaaat agagattatc tctatttaag aggagaactt
    27121 ttggactggg tgaaaatgca aaccgagtgg ctaaagactg tttttcttgc agggagtgtt
    27181 acttctttca aggaatatgc aaaggcaaag cagaacaatg gaacagggta aactgtattt
    27241 agtctgttat gattttccct cctcaaaagc aggcgatagg agaagagtga aggtggtaaa
    27301 aattttagaa ggctatggaa acagagtgca gaaatccgtt tttgaattgc gaattcataa
    27361 gaaagaagaa ttagaccagc tattaaaaaa aatcaaacgt tggtttatag aagcagagga
    27421 ttctttgcgt atctatcctt tgaatgcaat tgcagaaagt gaaattcaaa tccacggaga
    27481 aggggaaatt tataaagtag aagatgccta ttttttttaa gtatagcttt acagaaaaat
    27541 ggatttggaa ctctccaaat ccagtcctac acaccatccc ttatctcaaa atccaaagaa
    27601 atttattgac taaatccttt tttggtcatt ttgtatggtt tttcgatcta aatctactag
    27661 taaattttac aaacacagcc gattttagta gagtgagtag gtataaattc ccttttctca
    27721 gaaagtttga ttttaggctt agaaaaggga aagccaattt aaaatgactt gctacccccg
    27781 ttagaatgca ttattttggt agttgggatg ggtactctaa cggatgagtt tcctcgaatc
    27841 agaggattgc gactgttcta acccctgagc cttgtatttt ggttttaaag attgactaac
    27901 ggatgagttt cctcgaatca gaggattgcg acatcgacat ggagacagtt gagaatttgt
    27961 ttttctttct tgctaacgga tgagtttcct cgaatcagag gattgcgacc atccacaaaa
    28021 catcggctaa tggtacttat gcagtgatct aacggatgag tttcctcgaa tcagaggatt
    28081 gcgacttata tgtatttgga actagtgcaa atagctcttt ttcagctaac ggatgagttt
    28141 cctcgaatca gaggattgcg acaagcgtgt tgagcttcat gtagcactag agttataaag
    28201 tcctaacgga tgagtttcct cgaatcagag gattgcgact tggaagttca agtgtctctc
    28261 cataaggaag ttttatcaat ctaacggatg agtttcctcg aatcagagga ttgcgacttt
    28321 taacaagata aggataatat ctagtataaa aagttgacta acggatgagt ttcctcgaat
    28381 cagaggattg cgaccctgga acagcaatcg gggtgttagg tgatttcaat tctagctaac
    28441 ggatgagttt cctcgaatca gaggattgcg acatttctta aaactaaagt ttccattttc
    28501 actcttttcc actaacggat gagtttcctc gaatcagagg attgcgacgg tgtaatatga
    28561 accgcaatca aaaggaataa atcctttcta acggatgagt ttcctcgaat cagaggattg
    28621 cgactagaat atatttacca ttttctggtt tggtattatt ccatctaacg gatgagtttc
    28681 ctcgaatcag aggattgcga ctaagtcagc agttgcagcg atttcaattg cacgaatctt
    28741 tctaacggat gagtttcctc gaatcagagg attgcgacct tcatagattc ttcataattc
    28801 attactttct tttctccatc taacggatga gtttcctcga atcagaggat tgcgactttg
    28861 aatggaccgt tctagatcag cctcattgat atcacctaac ggatgagttt cctcgaatca
    28921 gaggattgcg acttgaatag atcaaactcc ccgttgtata gttctccatg cgctaacgga
    28981 tgagtttcct cgaatcagag gattgcgact ctcattatgc ttcgctgtaa aataaacaac
    29041 cttatctatt ctaacggatg agtttcctcg aatcagagga ttgcgacttc ataatccaac
    29101 tcttcctgga aaaactcttc tggtctaacg gatgagtttc ctcgaatcag aggattgcga
    29161 cttcaaatac atgagtaccc atgtgttgta tcaattctgg gctaacggat gagtttcctc
    29221 gaatcagagg attgcgacta ggatataact taccttcgta aataactcca cacatatgga
    29281 ctaacggatg agtttcctcg aatcagagga ttgcgacacg atctaaatta gaagctttag
    29341 caatagcact catttcttct aacggatgag tttcctcgaa tcagaggatt gcgactcggg
    29401 attaacctta gcagctttac aagcggcttc gatcactaac ggatgagttt cctcgaatca
    29461 gaggattgcg actacaaaca atagtcccat cctctctttg tcttaatact tgctaacgga
    29521 tgagtttcct cgaatcagag gattgcgaca ttgggaattc aaagttgatg acgtttccgt
    29581 ttttttctaa ctaacggatg agtttcctcg aatcagagga ttgcgacttt ggtaaaacgg
    29641 ggttaagttt tgtcatataa actcacctaa ctaacggatg agtttcctcg aatcagagga
    29701 ttgcgaccaa tactactact tcggatccat ttacttttag aacccatcta acggatgagt
    29761 ttcctagaat cagaggattg cgacctcgta atagatcaac actaaatact ctaatgatgg
    29821 cagctaacgg atgagtttcc tcgaatcaga ggattgcgac gtaaatggca actatcgcaa
    29881 gcaaagcaaa aagaaattaa ctaacggatg agtttcctcg aatcagagga ttgcgacgaa
    29941 agtgtaacca cttaacattt ttattcgata agaaaagcta acggatgagt ttcctcgaaa
    30001 tcgaaccccc ctttgctctg atggagcaaa cctagcaggc ctcacaacct accgcctgat
    30061 ggctaacggt tgttcgccgg aatgcgaccg cttgtttgtg tagttttgct ttgtagtttt
    30121 aagggtagtg gataagatag atttcctcga atcagaggat tgcaacgtac cagactcaca
    30181 gctgaggtcg aagttataga tgttctaacg attaggtttc ctcgattccc ccctttgctc
    30241 tgatggagca aacctagcag gcctcacaac ctaccgcctg atggctaacg gttgttcgcc
    30301 ggattgcgac cactggtttg tttagaattg ctttacagtt ttaagggtag tggataagat
    30361 ggactagcct cgattgagag gattgcgaca tagggtcgcc attttcatct attgtagatt
    30421 caggctcgct aacgattagg tttcctcgat tcccctcttt gctctgatgg agcaaacagg
    30481 caggtctcac aacctaccgc ctgaagggct aacggttgtt cgccggattg cgactgcttt
    30541 tttgtgtagt tttgctttgt agttttaagg gtaggggata agatagattt gcctcgaatt
    30601 agaggattgc gacacctctt taccaattcc ctctaatatc caagtcattc gctctaacga
    30661 ttaggtttcc tcgacttcga accgcccttt gctctgacgg agcaaaccta gcagacctcg
    30721 gcactttccg ctgatgctat cagtgcctcg tcggattgcg accgctggtt tgtgtagatt
    30781 tgatttactg ttttaagggg aggggataag aaggatttac ctcgactgag agagaatttt
    30841 tcttcttgaa ccgccccctt tcgtaaaggg ggaataaagg ggattagaag aactaacgta
    30901 tcctagaatc agaaggattt aaaatctctc ccttactact tttgctatca ttacgcatgt
    30961 atccatataa acaataaatc ttttaatttc acgctagcgt gtttgtaaat ttccaataaa
    31021 tcctatacaa tatcgatttt cacctaatgg aaataggtgt gaaatttata tattagtaag
    31081 gagaatatat acatggcagt agaacaaagt gaaatttcat ctaagagtag attgattaca
    31141 ttactactta gttttttatt tgtgcataga ttttatgttg gacgaatcgt ttccggcatt
    31201 ttcttttttt tcacagcggg tggttttggt ttttggtggc tcattgacgt tatacaaata
    31261 ctatccggct cattcaaaga tcaatttggt aaattcattt taaattggga agcggataaa
    31321 aagcaattga ctctatctgg tgttgccttg ggatttgtag ttctattagc aatgcttgga
    31381 ggtaaaaata aaaatgggga aacttcctct cccactttca aagatgcttt gcaatcttct
    31441 ggctctaatt caaccgaaga gaaaaaatct tttcccactg taggagacaa gattcaaact
    31501 tctcatttcg ctatcacttt gaaaggttat aaaattcaaa attccgtaaa aacaggaaat
    31561 atgtttagta aattagatcc cgaagaagga aacaaatatt tagttctcga tgtgaattac
    31621 aaaaacacag acaatgaaag tagaatgatc ggaggcgaag gaaaagtatt tgtagaaatg
    31681 aatggaaaag aattcgaata cgataaatcc gaacacattc tactcgatgg atggggagtt
    31741 cttctagatc aactcaatcc tatgacccaa aagaatacta aattggtttt taaaattcca
    31801 gatgaattaa aaggaaaaat ttactgggaa ccagttcgta gttctgatcg cttcttcgtt
    31861 ggagaagcaa aataatttaa ttcaccccct aatcttattt tcctatttca aagtttcacc
    31921 aaacaaaaaa attgtgactc ttctaaatca caatctcttc ccactctctg agtgggaaga
    31981 gtctatcgta ttcgtcttgg gatttagagc attaaaatga gaaattatat tttgatagaa
    32041 aaatcgatcg aattattgta acaagagaaa gctttgagaa agaaacagcg ctaatctata
    32101 attcaataaa gaacaccggt agaatcctat ttggagaaat agatatgact ccaagtgatt
    32161 tgccttatag agagcgttat gcggaattat ataaccgttc taaagaaggt ggaattagaa
    32221 aagtagaatc tgccgaagag tttattttca aactaaattc aacggaaacc gattttctat
    32281 atattcctgc aaagcataca atccaagtgt atttggcaat gaaaggacta ggttttacca
    32341 gtaattttga aaaactctat gagttaacaa gacaggaaat cggtgaagaa tttgcaaata
    32401 tgtttcacta tttattttct ttaaatgaaa agcaatttga tgcaaccaaa gaagaatgta
    32461 ttcttggtct agagtatgca aaaaaaatat tggcacttta tagcagtgaa gtttagatat
    32521 gtaataaaag ggggcttgcc gggaagaaga aatggaaagc ctttgaaggt tttttctgtt
    32581 tgccagtgga atattttcca gctcgaaaaa cacgtaaggc tggttatgcg gtagaagttt
    32641 tcaatctaat tggagaaacg ataggcgttt atgatttgtc agaatcacag ttagaaccaa
    32701 ttccacagtc tgccgttcct tcgatacgaa agattcaaaa gatagccgtt taacatatat
    32761 ttctaaactc tttactgtgt tagaagattt cacattgaca acatattctg aatggaagga
    32821 agttgcagat aggattcgga acattctttc attcttgaca tttaatagga atctaaaaag
    32881 aatctaacta tgctatctgc tgaaaaaata aagcttctac aagaactaac caaagaaacg
    32941 aatccagatg cttcaataga aaagatcgtt caagactata ttgatacaaa gattgcttat
    33001 tggagaatgg ttgataagtt ttactcagaa aaaatgggta tgagttttga agtctatgag
    33061 gatacaaaaa gagaagaatg ggatggcagc gattggaata aaatcgaaga atttcatgac
    33121 tgggaagatg catttatcgg aatcaagtat tatttggaat taaaagataa atggatattt
    33181 cagacatcca actaaaatta gaatcaaaat taccgcagat tgattctatc gaagatattc
    33241 atttttatca agtggatgaa tattcaataa aaggaaatgc aactgttaag aaaatttata
    33301 atctaaatat tcgattcaaa acatttcatg agcgtatgac gctctctttt gccctgattt
    33361 attaaaatca aagaatctgg ggacttgacc gtgataataa aatcggatgg catattcatc
    33421 cattgggcaa tcctagctct cacgtttcta tcgaatcgca aacgatcgaa caaatagtgg
    33481 atattttaga atctattgtg tctcaaattt aaaggaaaat gaaaaactgc attgaatgtt
    33541 ttctcttttt gaggagattc ccaaaacacc tagctacttt tcaaaaagtc ccagctattt
    33601 ataaattttt agccttgact tttgtagaag ttaagttacg ttataaaaaa cttaaagcta
    33661 cttttgccga agtagcttta aaagataaat tgtgaaacag ggaattggga ttaggcagct
    33721 ttgatctcca tttccaattc gcctgttctt atttcttgcc cgaaactatt tttcactttt
    33781 agcttgtaac ttactcccgg attaagaccg ttaggcactt taaaggagat ggatttacca
    33841 gctatctttg aatactcgga tactctttgt tctactccag caccttcaaa gaatacaccc
    33901 acatttggaa ctgatttatc aaatttcaaa ttctccccgt ggatggtgac taagtcgcca
    33961 gcaactaaag aggcttctag tccagttgtg tgattttcca aagaaaaaag taggggaagc
    34021 gttttactca gtgagactct ttgtaatttt gcatcctgcg taattttctt tagaaaattt
    34081 ggatttggtg caatagagac ttcaatccag tttttttcct gcgaaaaact atcttctagg
    34141 ttttggaagc ttcctttcac ttgcggtcga atcgtgcaaa agcctaaatt cacagatcta
    34201 ccttgtgaaa gattttctat acatactcgc tccaagtttt ccaatacagc ctttacatca
    34261 gctaaagttg cagtggagcc gtaggcgaga gcctttagga attgttcgaa gactaaactg
    34321 ccttctaaca gggttttggg taaatacggg gaaactcctg ccgtagataa ccgattttta
    34381 atgagggaat acttaatcat ataagcatct ccttaggttt ggatttatta tgtcgcggac
    34441 ataatattct actaccttat attagtgcaa agaaaagtaa acaaaaaaat atatattaaa
    34501 aatagatatg tataaaaata attacatata gaaaaaaaat cttatcctat tttgatatag
    34561 aaaattcaga actattcctt gactcgaaac tcgttcgcta caaaactggt aattcgatcc
    34621 tttttagggc gggagaactt aaaaatggaa aatccgaagc aattttataa ggctattggt
    34681 agacgtattg cagatgcgcg tcaatccatg cacatgtcgc aagaagatgc ggctgataaa
    34741 ttaaatatta ctcgtgttac ttggaatcat atagaaaagg gaaatcaaaa actgacttta
    34801 gatcggttaa tcgatatagc aggtctttta caaattgcac ctactaaatt agttcccggt
    34861 cttgtttcaa gtgacgaaac ggatgtttta acagagaaaa atttctcctc agatgaacaa
    34921 gcactcatat tggaaagatt agataaatat aaaaaacaaa aaaagtaagg aattctttcc
    34981 gatcgtttga atcggggatt ccaacaaagg gagaactcta tggaatcaaa gtttgcgaat
    35041 gaaataaaga aattgttcac gctcattcaa tataagactg gagcagtgga tctaaataaa
    35101 atagcagaga aattaaatat tagagtgatt gaagatgatc taccagataa cgtctctggt
    35161 gtcttggatt gtagaaccaa ggatgatcct attattcttc tcaattcgaa tcatcatctc
    35221 aatcgacaaa gattttctct tgcacatgag attggtcatt ttgttttaca taagatgagc
    35281 ggagttcaca tggatacaaa aatctttctt cgctctgatg cgcctaacct tcatggtgtc
    35341 cgaattgaaa gagaggcaaa tcaatttgcc gcagaactct taatgcccga agaggaaatt
    35401 agaaaagcct ggaagaatct tccggaagat tggtttgata aaagtccttt ggaagtcatt
    35461 gcaaaacaat ttaaagtaag cgaaggcgct ctctttattc gattgaagca attaaaagga
    35521 attggtggta tttggtagac gtgcattatg attcacaaat cgaaaaagga gaagagtaaa
    35581 aatgattcaa ttgccaaatc cttcctacga aactgcaatt ccaatctttc aaaaatttaa
    35641 tgatttgcaa gctgtctatt tgtttggatc tgtcgcgaca ggtcaaacaa atgcggagag
    35701 tgatgtagac tttggatttg ttgcggataa aaatataaaa gacgaactga gtctagaatt
    35761 aatcaaagcg ggctttggaa attttagttt agtctatata ccagaagcaa cacctgtttt
    35821 acaatttgaa attgtagata aaaattttat cctctaccag aaaccagatt ttgatacatc
    35881 ttcctttcgc tcaaaaatta tcaaagtata tctagacatg gaatactaca aaggaattca
    35941 aagacaatat ctaaaggaac ggattctaaa tggaaaataa atcagtcata caaacaaaga
    36001 tagaactttt aaatgattat ctctcttttt tacaacaatc gcaaaagtat tcagaatccg
    36061 aatttataca atctatgatg attcaaggaa gtgtggaaag atatttacat ttatctatag
    36121 agactacaat tgatatggga aatcatatta tttcaaaaaa tggttgggga agtgttcatt
    36181 ggtatcaaga tatagcgact ctatttcagg agaagggata tatcgattct gaattggcag
    36241 cactatggat tcga
//
LOCUS       DAOQOS010000050        35372 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_498_length_35372_cov_5.632217, whole genome shotgun sequence.
ACCESSION   DAOQOS010000050 DAOQOS010000000
VERSION     DAOQOS010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 35372)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 35372)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35372
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_498_length_35372_cov_5.632217"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(140..577)
                     /locus_tag="PLF66_12490"
     CDS             complement(140..577)
                     /locus_tag="PLF66_12490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004476172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HMZ64907.1"
                     /translation="MLYFEDLVVDQRFELGSYKITKEEIIRFAKEFDPQPFHIDEEKA
                     KSSIFGSLIASGWHTNSIYMKLFVKEMMSRAHGMGSPGLEELKWKKPVFPDDTLSGIF
                     TIIEKKPFKGNLGLVRARNELMNQNGVLVLSFIGLMLFAKRPQ"
     gene            827..2857
                     /gene="ispG"
                     /locus_tag="PLF66_12495"
     CDS             827..2857
                     /gene="ispG"
                     /locus_tag="PLF66_12495"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020784592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(E)-4-hydroxy-3-methylbut-2-enyl-diphosphate
                     synthase"
                     /protein_id="HMZ64908.1"
                     /translation="MNLRYNIFPFSYKRMITREVKVGDVGIGGTNPIRIQSMTTSDTQ
                     DTNTTVKQILDLTEAGCEIVRVTVPSMADAENLINIRAELKKVHCRTPIVADIHFTPT
                     VAMKVVDFVEKVRINPGNFADKKKFAVREYTDAEYKEELNRIADIFIPLVRRCKELGV
                     AMRIGTNHGSLSDRIMNRYGDTPAGMCESALEFIRIAESEGFYDLVVSMKSSNPIVMV
                     EAYRLLASKFLDHDMNYPLHLGVTEAGNGNDGRIKSAIGIGSLLEDGIGDTIRVSLTE
                     DAVREIPVAKKLVEKYNDLFKKQLEREKSIPVPPAITVSDNVSFSEFRNPFVYERFYS
                     DSVPIGDLRIGEKYPIRVETAINIDNANVRYLENLIAEQEKINPNVKHEIFSFLIQNE
                     EELNKLLVLKKTAKIKIPFSANLGEFDSRILEHSHSLLQFDKIVFNPFHYNFRSLNFI
                     LFLKSFQDRRKTCEFKIPADDMNAIPELVKILRENHLKNYIFSLETNEILLDNRKLAY
                     FLQSSEFPILLFGKFENLEESLYKASVGIGGLFVDGIGDAIRIVSKTANPNQMLNLSF
                     DILQATRLRTSKTEFISCPSCGRTLFDLQETTARIKERTGHLVGVKIAIMGCIVNGPG
                     EMADADFGYVGAGPGKVHLYYGKEIVSKSIPSEEADEKLIELIKEKGMWKDP"
     gene            2854..4146
                     /locus_tag="PLF66_12500"
     CDS             2854..4146
                     /locus_tag="PLF66_12500"
                     /inference="COORDINATES: protein motif:HMM:NF015573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HMZ64909.1"
                     /translation="MIHFFITIVILGIASVVTQKIDILKRYRIPVSLTSSLIVLAFFT
                     GFPSLKELPIYATWKTWPSIFIALIFAALFLERSEGKQVTSEIRESLGETYLVWITIV
                     GQMVVGLFLSLILFKPFFNLPLAFTSVLETGFAGGHGTAVAMNQSYIDNGLPNGQEYG
                     LFSATVGLILGIVGGIILIRRERGAGIVKEEEGDKLNLDPLKVIITLTLIFGAFLIGS
                     LLKENILHLLDYIQADSFLSILKRLIPSLPLFVYALIGGVLVKAFLKLIQQENLIENS
                     IINLASNVFMELLIFSGIASIDMKVISDAIVPLAILFSIGFIWNVFCHYYVRPKLIKD
                     EYSFELSLINFGMVNGTTGIGLMLMKMVDPECKTKALKVYAEAAPFTSPFIGGGVLTL
                     SLPYILNIVNPYLVLLILIICIGTLFIIGKKSKQKYLL"
     gene            4490..5455
                     /locus_tag="PLF66_12505"
     CDS             4490..5455
                     /locus_tag="PLF66_12505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64910.1"
                     /translation="MKQKANINYTLPIFSILAALLINCPSSNEKKDDTTKNLLILSAL
                     SKSSSSSGSGTGTGTGTDGSSCTTNSDCNSSTVCVSGYNLNQTTTTSKCTTIPASSGT
                     ITINGTASSGTISSSAPIVDYTLTISTPGNYLITMFPTTSTLDSVLQIYDSTGRTSVN
                     SSIDSTLSGLRERVLYNFTTAGTYRIRAASYYLSGTFGGGFKVQVANTPSSVSGGGSC
                     NFFSNGTASSCYDFSSGSTYVSGACNGGTYNATSSCATVNAAATPTVSGRCLYAFNSI
                     FSNGHGLVVRNYYSAGTGIGAAVSTGQGSTDCNSIDGGSTNIIFQ"
     gene            complement(5925..6197)
                     /locus_tag="PLF66_12510"
     CDS             complement(5925..6197)
                     /locus_tag="PLF66_12510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64911.1"
                     /translation="MKVLKIKWSFLKKAPFRNFYFPISLTANSNDFSKPESFFTLSRA
                     FVNACSAAVGVREQERLLQVELEEQYEDAVHVYGIEEVQPPDVPAK"
     gene            6526..7767
                     /locus_tag="PLF66_12515"
     CDS             6526..7767
                     /locus_tag="PLF66_12515"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HMZ64912.1"
                     /translation="MPRGNGKDIILQAFHWNLVKTKGTGTMDWRHDSWYQVLTNMADE
                     IADMGFTMVYLPPPWIDDSIWEANGKHGGGEGYFWRDFNLNSRYGSKQELTALITKLH
                     SLGLKVIVDLVTNHRDGTRMQEDIWEHPGEHWSIGRRDTGGTFMDGKYDLALDNPKVN
                     NRFVSAMDELMQDCGVDGWRWDYVWGYAVEDVVSWIKRTKGEEYFSVGEYWQSSPYLK
                     NDPMIQKYGTNEADRILGWARDSNGCAFDICLKREIQSANPANLKYGLNLRKNPDERS
                     SVVTFVDNHDMGASPFSSANGWGQECWPCPPQFKSSAYAFILTAPGTPCVYWPDCFDW
                     GLKEEMKHLISLRKKAGIISKSDWIDLTDKYSGFAGIFLNEYGEESLALSIGSYFQNP
                     GSDWKVGYEKPGEWTVWIRNK"
     gene            7812..8573
                     /locus_tag="PLF66_12520"
     CDS             7812..8573
                     /locus_tag="PLF66_12520"
                     /inference="COORDINATES: protein motif:HMM:NF015909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64913.1"
                     /translation="MHLKDFENSPTLEKSAYDLVEKDLQERLYILFSEASKQRFATIL
                     ILEGWAGSGKGDLLKSITTRLDPRRIRVYSLENSDPKHNTHHFLYEYWNRLPLYGETS
                     IFDGSWYSRVSYEKQKKNISKSEYKDAFKSILNFEETLLNDRYIILKYFLNISEKEQK
                     KRFKKAIEEGKKWMVSDSDWEELENYDDYEKLFEEYINKTDSTICPWNVVSAKNKYYC
                     RISVMESIIRNLEEKLKIDSRDMLQILKSQEQQAI"
     gene            8573..9313
                     /locus_tag="PLF66_12525"
     CDS             8573..9313
                     /locus_tag="PLF66_12525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002973565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactose-lipid carrier transferase"
                     /protein_id="HMZ64914.1"
                     /translation="MTNALKFKDITFGESLGNKEYKKIIDELQEKARLFSHYSHKKKR
                     SIVLVFEGWDAAGKGGAIRRLTSRIDPRLYTVTSIAAPNEVERNHHYLWRFWTKLPSH
                     GNIGIFDRSWYGRVLVERVEGFTKEEDWQRAYGEIVNFEEDLTDAGSIIIKYWLHVSP
                     DEQLKRFNARQDDPLKRWKLTEEDWRNRDKWSLYEAAAEEAFTRTHKPNAPWYIIPAN
                     DKYYARTEVLRLFCDRLESELNIPSKFS"
     gene            9310..11730
                     /locus_tag="PLF66_12530"
     CDS             9310..11730
                     /locus_tag="PLF66_12530"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HMZ64915.1"
                     /translation="MRYFFFILVFFFLISCNPRLFKQSAVRGEIDASDISFQSSIVYK
                     LDGDFVFNWNELNTNFEKDENSIYINVPHSWNGFNWKGTEIDGKGYGTFKVRIKLPPN
                     APPLAITMKEQGTAFKVIVNNEIIKESGKVGKDYYSSMPRTVPLTLYLPKYNDHLDIV
                     VQISNYHYRKGGMWNPIILGTHEGITKYINFKRDMEIFLAGVIFITVLYHLGIYLFYR
                     IDTSSVIFSLFCFVVLVRLISCGYRLIAEIFPDLPYGFYSRLEFFSWFFAIPLGIHFI
                     CKIYPGITSPIFIRLFYSIAALFTSLLIFSPEFYSRTAIPSQIVGSLEFAFGFWVIIK
                     AVNQEREGSYLFFTGTIILLTVVANDLLYMNEIYRGEELGPFGILSFILFQSILLSKR
                     FLSAFIEKEKLKDTVTRELEEKVKIRTHELNQAKEEAEKANQAQKDFLANMSHEIRTP
                     MNGIIGMTEILLDSNLSDDQKDYVVTLKNSSQSLLVLLNDILDYSKIESGKLELVSQP
                     FNIREAIKDTVQLFLVQAKKKGIGIHLFIYEDFSENVMGDSTRLKQILANLISNAVKF
                     TEKGKVEIIAKKISETLDSDLISIQINDTGIGIPKDKQHLLFQRFMQANTSSTRKYGG
                     TGLGLAISQKLIQVMDGKLYIESEEKVGSKFTIKIPLKKSFHKMESIDDKTFTKEKPM
                     PRNLKILVAEDDSTNQKLIDLVLSKLGYTPVFVNNGLEATQIVREKSFDIIFMDIQMP
                     EMDGIDATKTIMHDPLVDKKPYIIAVTANAIKGDKEKYLAAGMYHYLTKPILIEDIKN
                     ILDELETK"
     gene            complement(11739..12185)
                     /locus_tag="PLF66_12535"
     CDS             complement(11739..12185)
                     /locus_tag="PLF66_12535"
                     /inference="COORDINATES: protein motif:HMM:NF015690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF309 domain-containing protein"
                     /protein_id="HMZ64916.1"
                     /translation="MKFDPEVNPILDAIAAKEDPDSTREYAFEKALEFYKKNKFFEAH
                     EIFEFQWKKEFGVQKLYLQALIQISVSMNKVFVNVNLVGAESQAKLAREKLLKVLNSP
                     ISKEATEYIKNILESLDEWIDWIQKKESLNSKKPEIISLEKILVLI"
     gene            complement(12175..13662)
                     /locus_tag="PLF66_12540"
     CDS             complement(12175..13662)
                     /locus_tag="PLF66_12540"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HMZ64917.1"
                     /translation="MDPFLNFYKKDLLSGYCTQEGTKEYFNRLGLNATSYSELDKLKI
                     SRIGFGSYRIFKGNSVFNKALEDSLLHGVNLIDTSTNYGDGSSESLIGDVLEELIDGK
                     KLSRSEVFVVSKIGYIQGRNLDIIEEKNFSDITYYDTNCYHCISPDFLEDQLERSRKR
                     LGLNTIDAILLHNPEYFLMDREKHNVEKDKAIEEYYLRIERAFEFLEEARQKGVIRYY
                     GISSNTFPVGEKEYTHTSLNKVLEIVESVRIKKNLSNSGFRIIQFPANLYEMNFAFEK
                     NNSGKTILEIAKEKNLFTLINRPLNAIAKSQRMDRLAIRSDININQIENKFEEYKKNL
                     KYFQENMFSKLEIEEEFTFLSILENYKNQFQNVEHFRQSLNYNILPHIRGFLNRVEKL
                     DREKKFYKDYVNLLNQGIQVFEVYLEACQNEKIKPLYERVKNLYSEFKDKTLSQISVY
                     SLLQFNGVNSVLVGMRRTEYVNDILPILSYPSISIEEKKFQNLEV"
     gene            complement(13664..14359)
                     /locus_tag="PLF66_12545"
     CDS             complement(13664..14359)
                     /locus_tag="PLF66_12545"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF013308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IspD/TarI family cytidylyltransferase"
                     /protein_id="HMZ64918.1"
                     /translation="MNNIFGIILSGGSGSRMKSDKPKQFSYLRDMTLLEHSVKSFNEW
                     GFFKSIVVVSNPDYILETENSISKYLLANDRIVAGGATRHESSLKGLEALHYDDKDIV
                     IFHDAARPFFLKSELHAVANAAVMYGAATVSDNVNETIVSCFEGKVENILKRDEINLI
                     KTPQSLHTSLLQILLKEKLEEEPTDLCSWALQFGVKTYLIRSNPFNMKITRDGDLELA
                     DRYFDLFRQLEQN"
     gene            complement(14352..16139)
                     /locus_tag="PLF66_12550"
     CDS             complement(14352..16139)
                     /locus_tag="PLF66_12550"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015681937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HMZ64919.1"
                     /translation="MSSNDSSKKLPSIRKSIIGSKRAGVSFPLLALLTRDSFEGGDFH
                     TLELMADWAKKTGVSIIQILPLNDLGNGKSPYSSISAFAIDPLYISLHKLGINIRSRK
                     ERIASLEINMLRIRELKIQNLRIHYNKIYNESLKEIIRNFMNQHPWIASYGAFKVLYN
                     RNGGAHWRDWNYGRDYTINLQKEIMKENEEEFYFILWIQYIAYQQLRDVKKKFEQEGI
                     FLKGDMPILTSSNSADVWARTDLFDLSLNSGAPPDNFSDEGQNWHFPIINWDKMKAEN
                     YAWWKERLEYLEHFYHLYRIDHVLGMYRIWAIPLSAKSAKFGYYHPQTGVSRKEFEEV
                     KLDADDFLAREIIYEFQKDKFIFYWDFYKLPGYYTLPEEVKKVLYPLSHKHLEEDEKE
                     WRREGEDVLNFFFENTEMQACAEDLGAVPSFVRDSIHENKIIGLDIIRWTRSFEDGSF
                     IPADKYRPLAVSSLSVHDTSIALDWWNSCSKEDQKSFAELIDLTEAEISATKVMKAML
                     RFALSTNSLFSINLLHDFMFDESYFPEKFSPNNMLAYPERHRINIPGTPEEKNWSYRF
                     PFLVEDLLANTGHIKEIRMLIDEAGRIHE"
     gene            16246..18174
                     /locus_tag="PLF66_12555"
     CDS             16246..18174
                     /locus_tag="PLF66_12555"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HMZ64920.1"
                     /translation="MTQNPKDIQANSNSEYSNFTKSKFTIRLKLLMIISTIILFSLSI
                     IISIATYVFKDDARTTIESNTLGTTLILTDNLTSKFLALSKNLKLVAEHISEDNGKST
                     NRDFKKIFFENDSDVLFLGVYRQEGEELILTEKLYNEGRETNASLEENDFISTANAMS
                     SYFSKSFAGATILRNASPIFKNPVLGLSIPFKEIKNKKTIIVSFIRLNGILESFQAAG
                     SSNNFLVDDEGNLLAHPNIDFIKAGKNVSSSPIVKSMLESNTNNKYQRFLDEDNSYYL
                     GSFKKLGFAAGGVISTVKEEKAFEQIYNLQRRNIYLMIIVLMLSILFVYFYANSLTNP
                     ILKLVDASHEVEGGNYHVKLPIGAHDEIGILTNSFNSMTKGLDEREKMKDAFGKFVNK
                     DIAELAMKGEIKLGGERKYCAIFFSDIRSFTAISEKLEPEEVVEFLNQYMTEMVNCIT
                     KTNGIVDKFIGDAIMATWGALRHDDNAVENAINGTLMMRDALRKFNEGRGGDKKPIIQ
                     IGAGINSGYVISGQIGSNERLEYTVIGDAVNLASRIESLNKPMGTDILISEEAYNKVK
                     DIFHVEKMQQIKVKGKEDPQTIYAVLGRKDDPNCIQTLVELRKIMNIKYEPPAPAPKG
                     KKKKSDDEEKEVKYEILE"
     gene            18158..19828
                     /locus_tag="PLF66_12560"
     CDS             18158..19828
                     /locus_tag="PLF66_12560"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HMZ64921.1"
                     /translation="MKSWNKDWNIPAFLIFNIILFAGIFYFDLHRSINVGSFEPIGTV
                     SFKINSILRKFDSSVVWYSIQTNSPLANKDTIKTYPESDAMIHLNNGTEIRVDEDTMI
                     YLDYSGNKPSINFEGGNIQINSSNSKDKNNEINVKTKDKTITLSGGDAKIGSGNGDDV
                     SVTVDKGSATIEGGGVNRKINENEIATISKSGISTRKLPYSLIEPENQRKFIATSTPF
                     SVNFKWAPTGSHTEPTFELSRVKDFKRVDTRLKNVTSANLSLAAGVYYWRVGGKNESG
                     QIEYSDSKKFILIKEVGLKLFSPASGANVASSSSGARVTFSWTPSETVSDYVVDISNS
                     PDFAPILRSVPVGTNTISMGNFTEGTYYWRVTTKPATGIPSIRSNPSSFSIGTPKPQE
                     AKVVDNKPILEEKQKEEKQKEEAKKVEEAKKAEAKKVEESIAGIAPEHLSPSSGKIDI
                     TKSKQIHFKWDKVKNAAYYILKIAPAEKKGNTLISERTSANGYTLKNFSKFSEGTFLW
                     EVTPFDKKNNSGKTSRGKFTIDLSDDGLKNLKPEEIKILSPDTIYRDK"
     gene            19829..21760
                     /locus_tag="PLF66_12565"
     CDS             19829..21760
                     /locus_tag="PLF66_12565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64922.1"
                     /translation="MMKNKKNNFTILYKISLILSFLSIILFSFELFAKDTGFIEWKAI
                     PAANGYRVEIKSAGKIISETNVSSNIHYVDLPKGNYEFRIGVLNFFKKPVVWSYWNPL
                     KVIISQIPILVSEREEKVIGEPLVIEGDHFLENTKVSLIKDGVTTNVDSKIVGEKQIS
                     IPTDKMQVGTYDLILENPNNKKLTKKEFLVLYNSKGGIRRKAPEKIIKKKDIEISSES
                     LEKADVSFDESEPGKTKINIGPKSKEKLDINLENKSNLDVSVDINNRSAGKVNLNVEN
                     KSKDKITLNVTNHSKEKMIVGVIDKSKGKIDVNITPKSKQKVDIDTIFADIEDFDVTI
                     ENKAKSKFEINIGKPAPEVIVPKRTPARIINRNDISVSADSLDKVDIAVDDTKPNKLS
                     VDIGPHTEEDLEISLENKSKENVAIDLNNKAKGSVKLLVENKSDENLAINVNNASKEA
                     LMVEILDASIGNVDITKDEKSKGRINISRSEKVVEYFDVTIENKTRDKFDINIGKKEP
                     DPLVFKKSNEVFPRDYYTYSLKKYQDFLNGLKRTCSSSLDIPDILIEECYSRHATLNL
                     EDIDRQVLYNFIKLNSGNYISRIGAYKFFIENCSPPLAFILELADYRLKNLTMDENEI
                     FYIKKMKESFSSCRSKDGQ"
     gene            complement(21729..22844)
                     /locus_tag="PLF66_12570"
     CDS             complement(21729..22844)
                     /locus_tag="PLF66_12570"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HMZ64923.1"
                     /translation="MNQQALILIVDDTELNVILLNQILLNAGYRVLTANGGRKCLEIL
                     ETEVPDIILLDVIMPEINGFDVAEKVKSNDKTKDIPIIFLSAVSDFDSKIKGLERGAV
                     DYIIKPYDDREVIARIEVHLKIKALEQERLKHINELEKLNTEKDNLLQVMAHDIRSPL
                     TSIMIMSEFLMSSNTTLDSEKVLKYSNTIFHSSQKILQLINQLMQSIKTEVAKIELTK
                     FDLVRSINHSIDLLARSLEKKSIHLEVKYFSESIPVLLDESKVDQILNNILYNAVKFT
                     NTNGKISLIVEKEIKEGKEYLHISIADNGVGIPKSILNELMTKKFSSHRAGTLGEEGT
                     GLGLSITKNLIAKLNGKIEIESEENVGTIVHLYFYSY"
     gene            complement(22846..24675)
                     /gene="uvrC"
                     /locus_tag="PLF66_12575"
     CDS             complement(22846..24675)
                     /gene="uvrC"
                     /locus_tag="PLF66_12575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012389048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HMZ64924.1"
                     /translation="MELERLNIIKEKIKNLSDSPGCYLWKNEKAEVIYVGKAVRLADR
                     VRSYLNPNITDVKTLALQNEIDDLDWIATYTEEEALILEDNLIKRYNPKYNIRLKDDK
                     RYPFICVSTDEPFPMVYLTRRIRADKKKYFGPYTDVKATRDLLDTIHKVFPIRKTEQK
                     LPLPKPRRPCLNFHIKRCLAPCQGNISEEEYRVIVDQIVKFLEGKKDSLLEELRIRME
                     AHSERLEFERALIYRNMIENIQSIQKRQTVINQAGGDEDIIAYAKREDDGQIVILEVR
                     DGRMESKKSFALNGLKLSSDEEVFTSFLKLYYLQANFIPTSIVLPQSVKKDIEVLFEF
                     LAKNLGFKPKLKFPSAGEKKALLGLAAKNAEMNLSERLLATKLKDQTNALKELKENLK
                     LKQLPRVIECYDISHFQGTNPVASGVMFVDGKPYKAGYRRYIMKSYSGINDPGMMHEV
                     IARRLQRLVNENESLPDLIVIDGGEVQLARATEAAIALDLPDLPMIGLAKKREEIYFP
                     GNKNPYSFDINSPSMRLLRHLRDEAHRFGVSFHRERRNKSQLKTALDGIADLGIQRKK
                     AILKYFLGKKKLANVNLSELKEIPGIGDKLALKIYESLNQLKK"
     gene            complement(24680..25672)
                     /locus_tag="PLF66_12580"
     CDS             complement(24680..25672)
                     /locus_tag="PLF66_12580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004451934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HMZ64925.1"
                     /translation="MQSKILPCCAFTPLGQKKKHITREEIHLRIKKGETVFPWKAEFK
                     EESREYPSHLELLEKYDIPYIIDIHSHFFPEIVMKLIWRWFDSVQWGIAYRYSVDRCI
                     EYLKRNRVYFYTTLNYAHKSNMAEWLNDWIFSNSKNWKGAIPFGTFYPEKGVLSYVRK
                     AVEEYGLRGFKLHCEVSKLDLNRTELEDTFYYLEEKQIPIVIHSGHAPLPGEFTGIEY
                     FSRFMKKFKNLSVIVAHMGAYEISDYAALLDIYPNLKLDTTMVFVDFLATGEDTDEFL
                     PILDRYSEKILFGSDFPNIPYVLSHPIRKLLEAPLSRVTKKNIFYKNAMNLFSL"
     gene            complement(25662..27728)
                     /locus_tag="PLF66_12585"
     CDS             complement(25662..27728)
                     /locus_tag="PLF66_12585"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HMZ64926.1"
                     /translation="MNLDKLSVIFLNLFIISFPLSVTVSQSFAFLSIVTFLYSSIQHK
                     SLMITLRNRAFIASLGIYFFLLPSFFWNIKEYQWKEIASVFTRSEFSDFWMCFIVLPA
                     FYHIRNLEYRKVMIRFFFISVGLVIVSGFISIFTPFRLAKFITDGFQVKEGVRLQHFA
                     GDLWGRYTYLPIGLMNTHLTFGGLCGLFFPGISSYFLLTIPNRKTGFNLLLGFVLLVF
                     SIVLFYNQSRSIWLGLFFTFGLIAVKLTTQIDFGKVQSNWKILFLFFGVFIFILIVSV
                     SIYKKNWLLQRAFQEGLKDNTTENQRYFIYKNTFAMIRDHWGMGVGPGRFDKEHLRKS
                     EEMISQNEQLWYELFITPRQHAHHDPLHFYMIGGIFGLVTVLHFWFYLFRLFLKNSLT
                     DQTVLFSGILVLFVAGFFQCYLLDDEVALPFFAFIGLFGGSLQKEDARSKAMEGIKAR
                     KKANLEDSFLVESLSLGNRISYLKNKIALNGKNSPFEKYNFFTLCILFIPLIVSISFI
                     LFKIRLDPMYVYNRKITLQNLEDKILVQESLRGKQVLFPTSHMKEKDFIRIEGCLSHR
                     FGKPILIRKEPFQLSLSLFNTENPPKQVFIRVIERDAFDQDQLYRVHQFKEIGDEYKF
                     DLNPHQKNRIRLDDPKLFQDVKVSSRFPENIYFRDFAFHFEGFDKTKESFDFPQINFG
                     DLCNAE"
     gene            27747..28577
                     /locus_tag="PLF66_12590"
     CDS             27747..28577
                     /locus_tag="PLF66_12590"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016545021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HMZ64927.1"
                     /translation="MFSLDKKENKKINFSVAIITYNEEANIRECIESVNSLADEIVVL
                     DSNSTDKTLEIAKTFEKVKIYSQKFKGHIEQKNDAILLCQGEWILSLDADERATPELL
                     KNIESTLNNPNDLNGYKVSRLTFHMGRYIRHSGWYPLYRYRFFRKGFAKWTGENPHDY
                     IEINGKGDKLKGDIIHYSFKDLSHQVDTINKFSSIVAFTRSKKGISFSVFRSLIKPLS
                     KFLEIYIFKLGFLDGYPGFVIAISSAYSTFLKTAKMYEMEKEFIKRPSNLRPDYGNEN
                     "
     gene            28567..29331
                     /locus_tag="PLF66_12595"
     CDS             28567..29331
                     /locus_tag="PLF66_12595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64928.1"
                     /translation="MKIKNIFKKFFPKRHLADNPEKPQRPPKRPKGFERELEDLKLKL
                     TDFLLTLKVNSGLVVKRNMYSLTKNGHGLYRLEGIEKTGRAFSIIVSTANHLQLNAEG
                     KVTGLIQVNEAELNRVIQNEYSSLKAMFDKFRNLNLNDKALEILDEKNKFNWKEIILW
                     NRYWKEMLFLHMSANHIALLIISLGDSFKNVYLEVATKKQKQIISDELFYLNQGVNSE
                     EMNPNSKNLNLMNFDIAMEEFKKSIQKIKKKMDLES"
     gene            29361..30092
                     /locus_tag="PLF66_12600"
     CDS             29361..30092
                     /locus_tag="PLF66_12600"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HMZ64929.1"
                     /translation="MDSSRRNILKDFSFELRKDSIHAVIGESGSGKTSFANTLFHLTK
                     KDLNLKYESYSIFGKDYSDYQESDWVKMRGKQITMIPQSPGKSFHPYISIGSQILDYF
                     KNKEPNLAQKDSILSLLEQVQIRNPEAAFHSLPKNLSGGEKQRILVAMAIAVKPEIVV
                     ADEPTTALDSINEKLTLNLLYTLLKNSGIGLVLITHDMRIVRELADDVTVLRSGELIE
                     KITLLNKNMNKLQSEYSQKLVSKSL"
     gene            30114..31664
                     /locus_tag="PLF66_12605"
     CDS             30114..31664
                     /locus_tag="PLF66_12605"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HMZ64930.1"
                     /translation="MLFRNLIKVSSIKIVLGFLMPLGLVADSKVQFEKLDTTRYPLVH
                     IYMQENKAKPLQGENIHITETQEGFTRQVQTINILKSNQLRPIRLILSIQASNEVDRL
                     QYSKQLATSFVQSLGKEDRIGLHIFARDTYFLNLNMDKAEALEKIDAISQGSGNRTNY
                     ALNFLLSQIREDELPNLLFTITTDKEVEVDDPIASVIKKSRGLKLPIHMLALSERKYL
                     NIADYSEGDFYPIEKNTSIALMQSQLYSFRKMPPIIEYKSPFAENVKLFRPTSIDLKL
                     KVGESDFKASYELTLLSFIKSKFANIEFFYSFMLVVLLICILLLYAVTKRAREIARRE
                     EERRREEELLKNDLYYHENMSTDTTESPTFKSYSSYEDETVIDETEDLYSYNEDSLSE
                     SPTQRDLDNLDDGYDVSPDLLESGEKYERGILILKEGPNPGRQFTINKPEITIGSTAS
                     NDLVLWDSSLSPMHAKIKRVKNIFILFDTVSNSGVYLNGKKLLRPKALYDFDEIRLGN
                     SLLLFRGK"
     gene            31666..32211
                     /locus_tag="PLF66_12610"
     CDS             31666..32211
                     /locus_tag="PLF66_12610"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016279 - protein-lysine
                     N-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein"
                     /protein_id="HMZ64931.1"
                     /translation="MQYSENILKKTKSESYVSPKVKCVSIEGKGKGLIAKEKISKGEI
                     VSISGGLIFKEEDHQKELVEKGIGDYCYYIEEGFLICPINPEDPSDDWRMNHCCEPNC
                     GVRGQIVFVALRDIEINEELTFDYAMTETDPNYSVPLSCDKSSCRKTFSGNDWKNPDI
                     QKKYKGHMSLYIERKIQNNET"
     gene            32201..32800
                     /locus_tag="PLF66_12615"
     CDS             32201..32800
                     /locus_tag="PLF66_12615"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HMZ64932.1"
                     /translation="MKHEILKDRVFTISNFLTVSRAFFLPFFIEYAYLYHLDTSIIKY
                     YYILISICGYVVLSDYLDGLIARLLKEETILGRYLDPVSDKIVTIGGLSTITAYFHFP
                     LWILILYIIREIIGVWVGLFLYFKRGLQGKPNIWGKWGVALVAISVVHYLSLPLLQRN
                     YPNLVFTKEPEISGYLLIAVVFIGSVKYLTDYKKDLVKE"
     gene            32922..34058
                     /locus_tag="PLF66_12620"
     CDS             32922..34058
                     /locus_tag="PLF66_12620"
                     /inference="COORDINATES: protein motif:HMM:NF021446.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2157 domain-containing protein"
                     /protein_id="HMZ64933.1"
                     /translation="MNLIKKLNLWKDAGLISDIQYASIIKYEEEHKPKNRTAYTIITL
                     GALVICLGIISLIASNWEYINDGVKLVIDFLILGGISYSIYIQRENNKKWLLEVLIIS
                     YFIMILASIGLISQVYNTGGKLYQAFLFWCLITLPLVFQSSGRATTHIWLIAFLFSST
                     SFLFDRISLFNKDEVAVVWLFSFLPTSLLFISFPLKQSKVDTLTVFGKTCLFWSILGF
                     LSGTIVFSFLKDISKIKNDSGLLAIQGLLMISLLFSILSVYLSLGQNKKIAAFLGIGS
                     ILYLFMLSSHLFYFHSEFLDTIFFILIWFVIGLLFHLIDSKQLFEFAIVVIGIRFLVA
                     YFQLFASLALTGIGLIFSGILIIVISVLYIRNRDKITNWIGELI"
     gene            34055..34540
                     /locus_tag="PLF66_12625"
     CDS             34055..34540
                     /locus_tag="PLF66_12625"
                     /inference="COORDINATES: protein motif:HMM:NF025703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDYXXLXY domain-containing protein"
                     /protein_id="HMZ64934.1"
                     /translation="MNSKKIFLLALLFPFIALLLLTFYKAYSLQSGLRFILPISGYDP
                     INPIAGHYVTYQVEYGPMTCEGVDPKAKEESCICLSLAHDNHYFLPTCEEREIKRCDA
                     YLRGKCRYGRFEAGIEEYFIPEEKAKSIDETIRKGKSKIKISVQKNGTALVEDLILVE
                     D"
     gene            complement(34800..>35372)
                     /locus_tag="PLF66_12630"
     CDS             complement(34800..>35372)
                     /locus_tag="PLF66_12630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64935.1"
                     /translation="RGQVFLISGGNFSGKGAKMQAHFNLLNKAGFDAAFIGEDELSYL
                     EMNPALKNLKLPVIAERENSFDAVLEKIIKSEGIQFRISHILPDVEDTSIDVQLLFKN
                     GGSYDYLKAIDVKKPVYFFTNEELSSAFSFKKNIYTAKCPAPDMLGKLNLYFRNKNLI
                     RQKQEFIPLNTVESNRSWIEPDREIVNELK"
BASE COUNT        12319 a         5974 c         5760 g        11319 t
ORIGIN      
        1 ttcaattctg attttatttt agcattcaat gactcgacat actcccaaac aaaagagtcc
       61 ttgtataatt ttttctgtag atctttttta atttgatttg cggttacttt cgttatgccg
      121 ctcacaagaa caactggttt cattgaggcc ttttagcaaa aagcattaga ccgataaaac
      181 ttaaaaccaa tactccattt tgattcatca attcatttct agcccgaact aaacccaaat
      241 ttcctttaaa tggtttcttt tcaataattg taaatattcc tgataatgtg tcatcaggaa
      301 atacaggctt tttccatttc aattcttcta aaccaggcga acccattcca tgcgcacgac
      361 tcatcatttc tttcacaaac aatttcatat agatcgaatt tgtatgccaa ccactcgcaa
      421 tcaaagatcc gaatatcgaa gatttagctt tctcttcatc aatatgaaat ggctgaggat
      481 caaattcttt tgcaaatcga atgatttctt ctttcgtaat tttataagac cctaattcga
      541 acctctgatc tactaccaaa tcttcaaaat ataacatgtc caagcatcac cctcttctta
      601 tcaatttacc gattatgtat ttctttcttt aaagcaaatt aattcctgcc gattccactg
      661 acatccaaat taaaacaaac tatcaaaata attttttacc aaaaaatcaa tctagtatct
      721 catctttcta cttaccccaa gctaaactaa ttgtaattta gattatctga caaattatag
      781 cgaataatta ctttacgaaa atgcctatta gcaaaatggt taaaacatga atttacgata
      841 taatattttc ccatttagct ataaaagaat gatcacaaga gaagtaaaag tgggagatgt
      901 aggaattgga ggaaccaatc caattcgcat ccaatcaatg acaacctctg atacacagga
      961 tacaaatacc actgtaaagc aaattctaga cttaaccgaa gcaggttgcg aaattgttcg
     1021 tgtaactgtt ccatctatgg cagatgcaga aaatttaatt aatattcgag cagaacttaa
     1081 aaaagttcat tgcagaactc ctattgtagc agacattcat tttactccaa ccgttgcaat
     1141 gaaagttgtt gactttgtag aaaaagtgag aatcaatcca ggaaattttg cagataagaa
     1201 aaaatttgca gtcagagaat atacggatgc tgaatacaaa gaagaattaa atcgaattgc
     1261 tgatattttt attccactcg taagacgttg caaagaatta ggcgttgcaa tgagaatcgg
     1321 aacaaatcat ggttccctat cggacagaat catgaaccgt tatggcgaca cacccgcagg
     1381 tatgtgtgaa tctgctctcg agtttattcg aatcgcagaa tcagaaggat tttatgacct
     1441 agtcgtttct atgaaatcct caaacccaat cgttatggta gaggcatatc gactcttagc
     1501 ttcaaagttt ttagatcatg atatgaatta tccactccac ctaggcgtaa cagaagcagg
     1561 aaatggaaat gatggtagaa tcaaatcagc aatcggaatt ggatctctct tagaagatgg
     1621 aatcggagat acaattcgag tttccctaac cgaagatgca gttcgggaaa taccagtcgc
     1681 aaaaaaactg gtagaaaaat acaacgactt atttaaaaaa caattagaaa gggaaaaatc
     1741 aattccagtt cctcctgcaa ttactgtttc tgataacgtt tctttttcag aatttagaaa
     1801 tccatttgta tatgaaagat tctatagtga ctcagttcct atcggagatt tacgaattgg
     1861 agaaaaatat ccaattcggg ttgagactgc aatcaatatt gacaatgcaa atgtccgcta
     1921 tttagaaaat ttaatagcag aacaggaaaa aataaatccc aatgtaaaac atgaaatctt
     1981 ttcttttcta attcaaaatg aagaagaact caataaacta ttagttctaa aaaagactgc
     2041 caaaataaaa attccttttt ccgcaaacct tggagaattt gacagtcgta ttctagaaca
     2101 ctctcattcc ctacttcaat ttgataaaat tgtttttaat ccatttcact acaactttcg
     2161 atctctgaat ttcatactat tcttaaaaag ctttcaggac cgaagaaaaa cttgtgagtt
     2221 caaaattcct gcggatgata tgaatgcaat tccagaactc gtgaaaattc tccgcgaaaa
     2281 tcatttaaaa aactacatat tctctttaga aacaaacgag attcttttag acaatcgcaa
     2341 actagcttat tttttacaga gttcagagtt cccgatttta ttatttggta aatttgaaaa
     2401 cttagaggaa tctttgtata aagcgtcagt cggtataggc ggactttttg tagatgggat
     2461 tggagatgcc atccgaatcg tatctaaaac cgcaaatcca aaccaaatgt taaatttgag
     2521 ctttgatata ttacaagcaa cgcgtcttag aacttctaaa acagaattta tatcctgccc
     2581 ttcatgcgga agaacattat ttgatttgca ggagactaca gctaggatca aagaaagaac
     2641 gggtcattta gttggagtta aaattgcaat catgggttgc attgtaaacg gacccggaga
     2701 aatggcagac gcggacttcg gttatgttgg ggctggtcct ggtaaagttc atctctacta
     2761 cggaaaagag attgtttcta aatctattcc aagcgaagaa gctgatgaaa agctgataga
     2821 acttataaaa gagaaaggta tgtggaaaga tccatgatcc atttttttat cacaattgta
     2881 atcctaggta tagcgagtgt cgttactcaa aagatagaca ttttaaagcg atacagaata
     2941 cctgtatcgc ttacctcttc tctgattgtt ctagcattct ttactggatt tccaagttta
     3001 aaagaacttc caatctatgc cacatggaag acttggccta gtatatttat cgcacttatt
     3061 tttgctgctc tgtttctgga aagaagtgaa ggcaagcaag tcacctctga aatcagagag
     3121 tcgttagggg aaacatattt agtttggatt acaattgttg gacaaatggt tgttggactt
     3181 ttcctatctc taatactttt taaacctttt tttaatctac cgcttgcttt tacatcagtc
     3241 ctagaaactg gatttgctgg tggtcatggc actgcagttg caatgaatca atcttatatt
     3301 gacaatggat taccaaatgg tcaagaatac ggactcttta gtgcaacagt cggactgatc
     3361 ttaggaattg ttggagggat aattctcatc cgacgtgagc gtggagctgg tattgtcaaa
     3421 gaggaagaag gcgacaaatt aaaccttgat cccttaaaag tcattatcac tcttactctc
     3481 atcttcggtg cttttttaat tggatcactg cttaaagaaa atattttaca tttactcgac
     3541 tatatccaag cagattcttt tctatcaatt ctaaaaagac ttattccttc tctaccattg
     3601 tttgtatatg cgttaatcgg cggagttcta gtaaaagctt ttctaaaact aatacaacaa
     3661 gagaatttaa tcgaaaattc aataataaat cttgcttcga atgtatttat ggaattgtta
     3721 atcttttcgg gcattgcaag tattgatatg aaagtgattt cggatgctat cgttccttta
     3781 gctattttat tttctattgg gtttatttgg aatgtatttt gtcattacta tgttcgccct
     3841 aaactaatca aagatgagta ttcattcgaa ctcagtctaa ttaattttgg aatggtcaat
     3901 ggaacaactg gaatcgggtt aatgcttatg aaaatggtgg atccagaatg caaaacgaaa
     3961 gctttaaaag tatatgcaga agctgctccc tttacttccc cattcattgg aggtggcgtt
     4021 ctcacacttt cacttcccta tattctgaat atagtaaatc cctatctagt gcttttaatc
     4081 ttaataattt gtatcggaac ccttttcatc atcggaaaaa aatccaagca gaaatactta
     4141 ttataaaata agatttctaa aacgctattc catactaaat caaagattag aaaaatacaa
     4201 ctaactgaga ttatcccctc atgctttagt ttaagatttt actaaattct cctttattta
     4261 ataaattaga gtctttctta aacgccaatc aggaattacc cttctgaaat cgaaagaatg
     4321 gatagatatt ataaaaggaa ggcataaatc tcaggattta tgtttaattc gttttatttt
     4381 aatactcatg ttatccacac tttataccat ttttttaaaa atcacttttc ttaaaattgg
     4441 ataattctaa aagtgacagt aaatcaattt ataggaaaaa aaaatctcaa tgaaacaaaa
     4501 agctaacatc aattacaccc tacccatatt ttcaattctt gctgcactct tgatcaattg
     4561 cccaagtagt aatgagaaaa aagatgatac aacaaaaaat ctactgattc tgtcagcact
     4621 aagtaaaagt tcctcttctt ctggatccgg aacaggaact ggaactggaa ccgacggatc
     4681 atcctgcaca actaattctg actgcaatag ttcaaccgtt tgtgtaagtg gatataattt
     4741 gaaccaaacc actacgacct ctaaatgcac aaccattccg gcaagtagcg gaactataac
     4801 aatcaacgga actgcatcta gtggcacaat ctcttcctct gcgcctatcg ttgattatac
     4861 gctgactatt tctacacctg gaaattatct aattaccatg tttccaacaa ctagcaccct
     4921 tgatagtgtt ttgcaaattt acgattctac tggaagaacc tctgtcaatt catccataga
     4981 ttccacttta tctggattaa gagaaagagt tctttacaat ttcacaacag caggcactta
     5041 cagaattaga gctgctagct attatctatc tggaacattt ggaggcgggt ttaaagttca
     5101 agtagcaaat actccgagtt ctgtatcggg tggtggaagt tgcaatttct ttagtaatgg
     5161 aactgcaagt agttgctacg acttttcgag tggttcaact tatgtatccg gagcttgcaa
     5221 tggtggtaca tacaatgcaa ctagctcttg tgcaactgta aatgcagcgg caacacctac
     5281 ggtcagtggt cgatgcctct atgccttcaa ttcaattttc agcaatggac acggacttgt
     5341 tgttagaaac tactatagcg caggaactgg aattggagcc gcggttagta caggtcaagg
     5401 atctacagat tgcaattcaa tcgatggcgg tagcacaaat ataattttcc aataaaaaga
     5461 aattatacga aagagacttg gtttcagaaa aatcagtctc tttttgttcc atcctaaaga
     5521 tcctctcttg ttctctgttt tcgaaagaga tgaaacgatc atctggaaaa atttgactac
     5581 tgtttactct atctatattc ttgttataaa aattttattt ataatataaa ttacttaatt
     5641 ctaggtagtc ttgatttctt taacggaaac taaaaaaata tttacaaaat aatcagaatt
     5701 gagtgtgtat gcctatagga gtaaattaac tatgaaaaaa aatttatatc agtgatttgt
     5761 ttaatcacgt tagtatttgg gatgcttttt agtgctggat gcggtacttc taaagcagat
     5821 aaggaagaaa aagataataa caacttgctc gttctattgt tattactagc atcttctaga
     5881 agttctgcaa cttcttcatc ttctggttct agcagaacta catgctattt tgcaggaaca
     5941 tctggtggct gtacttcttc aattccatac acatgtacag catcttcata ttgctcttct
     6001 agctctactt gtagcaacct ctcttgttct ctaacaccaa ctgcagcaga acaagcgttt
     6061 acaaatgctc ttgataaagt aaaaaatgat tctggtttac taaaatcatt agaattagct
     6121 gttaaagata taggaaaata aaaattccta aatggagctt tttttaagaa gctccatttt
     6181 atttttaaga ctttcaatcg aatctttctt ttatcaatag aattcctact ctcattagta
     6241 gcaaacctac agacctccca actctgaatt gaaaactaaa taaaattcta ataaactcaa
     6301 acttaaatta tctgccgaaa gaatactctc atttaaaaaa gtatttgttc taaacacttc
     6361 tgactctttc ttttttcaat gtgcattttt aaaagatcct aatcataata attttttgga
     6421 tgaaaattgt tttgtatttt gctatagaaa accaattctg caaaaaataa ctatttcctc
     6481 tttacaaacc ttaaccaatt tataaactaa gggcaggaga agatcatgcc aagaggaaac
     6541 ggaaaagata tcatattaca agcatttcat tggaatttag taaaaacaaa aggaaccggc
     6601 actatggatt ggagacacga ttcttggtat caggttttaa caaacatggc agatgaaatt
     6661 gcggatatgg ggtttacaat ggtttacctc cctcctccat ggattgatga ttctatttgg
     6721 gaggcgaatg gcaaacacgg aggtggcgaa ggatatttct ggcgagattt caatttaaat
     6781 tcaagatatg ggagcaaaca agaattaact gcactgatta caaagcttca ttctctcgga
     6841 ttaaaagtaa ttgtagactt agtgacgaat catcgcgatg gaactagaat gcaagaggat
     6901 atatgggaac atccgggaga gcattggagt atcggtaggc gcgataccgg tggaactttt
     6961 atggatggta aatatgacct tgcattagac aatcccaaag taaataatcg gtttgtttct
     7021 gccatggatg aactcatgca agactgcggt gttgatggtt ggagatggga ttatgtttgg
     7081 ggttatgcag tggaagacgt tgtgagttgg attaaacgca caaaaggaga agaatatttt
     7141 tccgttggag aatactggca aagctccccc tatttaaaaa atgatcctat gatccaaaaa
     7201 tacgggacca atgaagcaga tagaatctta ggttgggcaa gagattcgaa tggttgtgct
     7261 tttgatattt gtcttaagag agaaatccaa tcagcaaatc cagccaatct gaaatatggt
     7321 ttaaatcttc gtaaaaaccc agatgaaaga tcttccgtag taacgtttgt agacaaccat
     7381 gatatgggag catctccatt tagttctgca aatggctggg gacaagaatg ttggccctgt
     7441 cctccacagt tcaaatcatc tgcctatgca tttatactaa cggctcctgg aacaccatgt
     7501 gtatactggc ctgattgctt cgattgggga ctcaaagaag aaatgaaaca tttaatatcc
     7561 ctccgtaaaa aagcaggaat catttctaaa tcagattgga ttgatctaac cgataaatat
     7621 tcgggattcg caggaatctt cttaaatgaa tacggagaag aatcgcttgc tctatcgatt
     7681 ggatcctatt ttcaaaatcc tggttcagat tggaaagttg gatacgaaaa accgggtgaa
     7741 tggacagtat ggatccgaaa taaataaaaa actgttttca aaataaaata ttttttttaa
     7801 atagggatat catgcatcta aaagattttg aaaattcacc taccctcgaa aaatctgcat
     7861 acgatttagt agagaaggac ttacaagaaa ggctttatat tttattcagc gaagctagca
     7921 aacaacgatt tgccactatt ctcatactag aaggctgggc aggatcaggc aaaggagatt
     7981 tactaaagag tataacgact cgacttgatc ctcgcaggat cagagtttat tctctagaaa
     8041 attctgatcc aaaacacaac actcaccatt ttttatatga atattggaat cgactccctt
     8101 tgtatgggga aacatctatt tttgatggat cttggtattc aagagtaagc tatgagaaac
     8161 agaaaaaaaa tatcagtaaa tcggaataca aagatgcttt caaatcgatt ttaaattttg
     8221 aagagactct tttgaatgat cgatatatta ttttaaaata cttcttaaat atctccgaaa
     8281 aagaacaaaa aaagcgtttc aaaaaagcga tagaagaagg aaagaaatgg atggtatctg
     8341 acagtgattg ggaagaactt gaaaactacg atgactacga aaaattattt gaagaataca
     8401 taaataaaac agactcaaca atctgtccat ggaatgttgt ttctgcaaaa aacaaatact
     8461 attgtagaat ctctgtcatg gaatcaatca ttcgcaactt agaagaaaaa ttaaaaattg
     8521 attctaggga tatgctccaa atcctaaaat cacaagaaca gcaggcaatc taatgactaa
     8581 cgcattaaaa tttaaagata ttactttcgg cgaatctcta ggcaataagg aatataaaaa
     8641 aatcatcgat gaattgcagg aaaaagccag actcttttct cattacagcc acaaaaagaa
     8701 aagatctata gtattagttt ttgaaggatg ggatgcagca ggaaaaggcg gcgctatcag
     8761 aagactaacg tctaggatag atccaagact ttacacagta acttcgattg ctgcgccaaa
     8821 tgaagtggaa aggaatcacc attatctatg gagattttgg actaaactac cctctcatgg
     8881 aaacattgga atctttgatc gctcatggta tggcagagtt ttagtcgaga gagttgaagg
     8941 ttttacaaaa gaagaggatt ggcaaagagc ttacggggaa attgtaaatt ttgaagagga
     9001 tttaacggat gcaggaagta ttattataaa atattggctc catgtttctc cagacgaaca
     9061 attaaaacgt ttcaatgcaa gacaagatga ccctttaaag agatggaagt taaccgaaga
     9121 agattggaga aatagagaca aatggtcatt gtatgaggca gcagcagaag aggcattcac
     9181 aagaacacat aagcctaacg ctccttggta tattattcca gcaaatgata aatactatgc
     9241 aagaacggaa gtgcttagat tattctgtga tcgtttagaa agtgaattaa acataccatc
     9301 taagttttcg tgagatattt tttcttcata ctagtattct tttttctgat ttcttgcaat
     9361 ccccgcttat ttaagcaatc ggcggttaga ggagaaattg atgcttctga tatttctttt
     9421 caatctagta ttgtttataa attggatgga gactttgttt tcaattggaa tgaactaaat
     9481 actaatttcg aaaaagatga aaatagtatt tatattaatg ttcctcattc ttggaatggt
     9541 tttaactgga aaggcacaga aatagatggt aaaggctatg gaacatttaa agttagaata
     9601 aaactgccac ctaacgctcc acccttggca attacaatga aggaacaagg aacagccttc
     9661 aaagtaatcg taaataacga aatcataaaa gagtcgggca aagtaggaaa agattactac
     9721 tcatcaatgc ccagaacagt tccactcaca ctctatctac caaagtataa tgaccattta
     9781 gacattgtag tccaaatttc aaattatcac tatcgtaaag gtggaatgtg gaatcctatt
     9841 atactaggaa cccacgaagg aattaccaaa tatattaatt tcaaacgaga tatggaaatt
     9901 tttttagctg gtgtaatttt tattacggtt ctatatcacc taggaattta tctattctac
     9961 agaattgata cttcctctgt tatttttagc ttattttgct ttgtcgtctt agtaagactt
    10021 atctcttgcg gctatcgatt aattgcagaa atttttcctg accttcccta tggtttttat
    10081 tctaggctcg aatttttcag ttggtttttt gccatacctt taggaattca ttttatttgt
    10141 aaaatctacc ctggaatcac aagtccaatt ttcatacgtt tattctattc tattgcggca
    10201 cttttcacat ctcttttaat tttttctcct gagttttata gtagaaccgc aataccatct
    10261 caaattgtcg gttcccttga atttgctttt ggcttctggg ttattataaa agcagtcaat
    10321 caagaaagag agggttctta tctttttttt acaggaacga ttattttact tacagtagtt
    10381 gcaaatgatc ttttatatat gaatgaaatc tatcgagggg aagaactcgg tcctttcggg
    10441 atactttcct ttatcttatt tcaatcaatc ttactttcaa aacgatttct atctgcattt
    10501 attgaaaaag aaaaattgaa agataccgtt acccgcgagc tagaggaaaa agttaaaata
    10561 agaactcatg aactcaatca agcaaaagaa gaagcagaaa aagccaacca agctcaaaaa
    10621 gattttcttg cgaatatgag tcatgaaatt agaacaccta tgaatggaat tatcgggatg
    10681 acagaaattt tattagatag taacctatca gatgaccaaa aagactatgt tgtaacttta
    10741 aaaaatagca gtcaaagtct actagttttg ctaaacgata ttttagatta ttcaaaaatc
    10801 gaatcgggaa aattagaatt agtctctcaa ccgtttaata tacgagaggc aattaaagac
    10861 actgttcaat tgtttttagt tcaagccaaa aaaaaaggca ttggtattca cttattcata
    10921 tatgaagatt tttctgaaaa cgttatggga gattcaacaa ggctaaaaca gattcttgca
    10981 aatttaatta gcaatgcagt taaatttact gaaaaaggta aggtagaaat tattgcaaaa
    11041 aaaatcagtg aaactttaga tagcgatcta atctcaatcc aaataaacga cacaggaatc
    11101 gggataccta aagacaaaca acatctactc tttcagcgtt ttatgcaagc aaatacttct
    11161 agcacaagaa agtatggagg aacaggacta ggtcttgcga tttcacaaaa attaattcaa
    11221 gtcatggatg gtaaacttta tattgaaagt gaagaaaaag ttggatcaaa attcacaatc
    11281 aaaattccac tcaaaaaaag ttttcataaa atggaatcta ttgatgacaa aacttttaca
    11341 aaagaaaaac caatgccaag aaatttaaaa atactcgtcg cagaagacga ctcaaccaat
    11401 caaaaattaa tcgatttagt tttatctaaa ctaggatata ctcccgtttt tgtaaacaat
    11461 ggtttagaag cgactcaaat cgtcagagaa aaatcttttg atatcatttt catggatatt
    11521 caaatgcccg aaatggatgg gattgatgca acgaaaacaa ttatgcatga cccactcgtt
    11581 gacaaaaaac cttatatcat tgccgtaacg gctaacgcta taaaaggaga taaagaaaaa
    11641 tatttagcag ctggtatgta tcattatctc acaaaaccta tattgattga agatattaaa
    11701 aatattttag atgaactgga aacgaaatag attttgattt aaattaacac taatatcttc
    11761 tcgagtgaaa taatttctgg cttcttacta ttaagacttt cctttttttg aatccaatcg
    11821 atccattcat ctaaggattc aagtatgttt tttatatatt cagttgcctc cttagaaatc
    11881 ggagaattta aaacctttaa gagtttttct ctcgcaagtt ttgcttgtga ttcagcccct
    11941 actaagttta cattcacaaa aaccttattc attgaaacag aaatctgaat caaagcttgt
    12001 agatataatt tttgaacacc gaattctttt ttccattgaa attcaaatat ctcatgtgct
    12061 tcaaaaaatt tatttttttt ataaaactct agagccttct caaacgcata ttcgcgagtg
    12121 ctatcaggat cttcttttgc agctatcgca tctaaaattg gatttacttc tggatcaaac
    12181 ttcaagattt tgaaattttt tctcctctat agaaatggat ggataagaca gaattggtaa
    12241 aatatcattt acatattcag ttcttctcat tccgactaga actgaattca ctccattgaa
    12301 ttgcaataaa ctatataccg atatttgcga taaagtctta tctttaaatt cagaatataa
    12361 attttttacc ctttcgtata aaggctttat tttctcattt tggcaggctt ccaaatatac
    12421 ttcaaatact tggattccct gattcaatag atttacatag tctttgtaaa actttttttc
    12481 tctgtctagt ttttctactc tattcaaaaa gcctctgata tgcggaagta tattataatt
    12541 caatgattgc ctaaagtgtt ccacattttg aaattgattt ttgtaatttt ctagtataga
    12601 aagaaaagtg aattcttctt ctatttctag tttagaaaac atattctctt ggaaatattt
    12661 tagattcttt ttatactcct caaatttatt ttcaatttga ttgatattta tatcacttcg
    12721 aattgcaagg cgatccattc tttgactttt agcaatcgcg tttaatggtc ggttgatgag
    12781 tgtaaataga tttttctctt tagcaatttc taaaattgtc ttaccagaat tatttttttc
    12841 aaaggcaaaa ttcatttcat aaagattagc tggaaattgt ataattctaa accctgaatt
    12901 cgatagattc tttttaattc gaacagattc aactatttct aaaactttat ttaaagaagt
    12961 atgcgtgtat tctttttcac caacaggaaa tgtattacta gagattccat aatagcgaat
    13021 aacgcctttt tgtctcgcct cttctagaaa ttcaaaggct ctttctattc ttaaataata
    13081 ttcttctatt gccttgtctt tttctacatt gtgtttttct ctatccatta aaaaatattc
    13141 tggattatga agtagtatgg catctatcgt attaagtccc aatcgttttc tagaacgttc
    13201 caattgatcc tctaaaaaat ctgggcttat acaatggtag caatttgtat catagtaggt
    13261 aatatctgaa aaatttttct cttcaataat gtctaaattt ctaccttgaa tatatccaat
    13321 cttcgaaact acaaagacct cggagcgact taactttttc ccatcgatta attcttctaa
    13381 gacatctccg ataagcgatt cactagaacc atctccataa ttcgtggatg tatcaatcag
    13441 attgactcca tgtaaaagag aatcttctaa tgctttgtta aaaacagaat ttcctttaaa
    13501 aatgcgatag gatccaaagc cgattctaga aatttttaat ttgtctaatt cagaatagga
    13561 agttgcattt aaacccaacc gattaaaata ttctttggtg ccttcctgag tgcaatatcc
    13621 acttagtaaa tctttcttat agaaatttaa aaatggatcc aaatcaattt tgctccaatt
    13681 gtcgaaatag atcaaaatat cgatctgcta attccaaatc cccatcccgc gtaattttca
    13741 tgttaaatgg attagagcga atcaggtaag tcttcactcc aaattgtaaa gcccaagagc
    13801 ataaatccgt tggctcttct tctaattttt cttttagtaa aatttgaaga agggaagtat
    13861 gcaaagactg aggagtcttg attaaattaa tctcatctct ttttaaaata ttttccacct
    13921 ttccctcaaa gcaactaaca atagtttcat ttacgttatc tgaaacagtg gcagccccat
    13981 acatcaccgc tgcatttgct actgcatgca attctgactt cagaaaaaat ggtcttgcag
    14041 cgtcatgaaa gattacaata tctttatcat cataatgaag tgcttcgagt cctttcagag
    14101 aactttcatg tcttgtcgct ccaccagcta caattctgtc atttgccaaa agatattttg
    14161 aaattgaatt ttctgtttcc aagatataat ctggatttga gacaactaca atagatttaa
    14221 aaaatcccca ttcattaaaa gatttaacag aatgttctag taatgtcata tcacgaagat
    14281 aggaaaattg cttaggttta tctgatttca tcctactacc ggatccaccg gataaaatta
    14341 ttccaaaaat attattcatg aattcttccc gcctcatcaa ttagcattcg aatttctttt
    14401 atatgtcctg tgtttgctag taaatcttcc actaagaaag gaaatcgata cgaccaattt
    14461 ttctcttctg gagtacctgg aatattaatt ctgtgtctct caggataggc taacatattg
    14521 ttaggtgaaa acttctcagg gaaatacgat tcgtcaaaca taaaatcatg cagtagattg
    14581 atcgaaaata aactattcgt agataacgca aagcgaagca ttgctttcat aactttcgtt
    14641 gcagaaatct cagcttctgt taaatcaatc aactcagcaa agcttttttg atcttctttc
    14701 gagcaagaat tccaccaatc gagtgcaatc gaagtatcat gaacagataa agaggatact
    14761 gcaagtggtc tatatttatc agctggaata aagctaccat cttcaaaaga tctagtccat
    14821 cgaataatat caagcccaat gattttattt tcgtgaatac tatcgcgcac aaaagacggc
    14881 acagccccta aatcttctgc acaggcttgc atttctgtat tttcaaaaaa gaaatttaat
    14941 acatcctcac cttcccttct ccactccttt tcatcttctt ctaaatgttt atgcgatagt
    15001 ggatagagga cttttttaac ttcttctggt aaggtatagt acccgggtaa cttataaaaa
    15061 tcccagtaaa aaataaattt atccttttgg aattcataaa tgatctctct agcaagaaaa
    15121 tcatctgcat ctaatttaac ttcttcgaat tcctttcgac taacgccagt ctgaggatgg
    15181 tagtaaccaa attttgcaga tttggcagat aacgggatcg cccaaatccg atacattcca
    15241 agcacatgat caattctata aagatgataa aaatgctcta gatactctaa tctttctttc
    15301 caccaagcat aattctcagc tttcatttta tcccaattga taatcgggaa atgccagttt
    15361 tgtccctcat cactgaaatt atctggggga gcacccgaat ttaaactcag atcaaataaa
    15421 tcagttctag cccacacatc cgcactattc gaagaagtta aaataggcat atcacctttt
    15481 aaaaagattc cttcttgttc aaatttcttt tttacatctc tgagttgttg gtaggctatg
    15541 tattgaatcc aaagaataaa ataaaactct tcctcgtttt ctttcataat ttctttttgg
    15601 aggttaatag tgtaatctct tccataattc caatcccgcc aatgagcacc gccattccga
    15661 ttgtagagaa ctttaaatgc accataggaa gcaatccatg gatgctggtt cataaaattt
    15721 cgaataatct ctttcaaaga ttcattgtag attttattgt aatgaattct taaattttga
    15781 atcttgagtt ctcttattct tagcatattg atttcaagag aggctattct ttcttttctg
    15841 ctgcgaatat taattccaag cttatgcagc gaaatataaa gtggatcaat tgcaaatgca
    15901 gaaattgaac tatagggact tttcccattt cctaagtcat ttaaaggtaa aatttggatt
    15961 atagacacac cagttttttt agcccagtct gccattaact caagagtatg gaaatctcca
    16021 ccttcaaatg aatcacgagt taataatgca agcaaaggaa aagaaacacc ggctcgttta
    16081 cttccgatta tagactttcg gatggatggt aattttttag aagaatcatt tgaactcatg
    16141 gaaatcatac tgaaaaaata gtatttttgg taaacttaaa aaacaggttt ttttattgat
    16201 taatttatgg cttaactcaa aggtaattaa gttggtaaaa aaaatatgac ccagaatcca
    16261 aaagatatac aagcaaattc aaactcagaa tattcaaact ttacaaaatc taaatttacc
    16321 attcggttaa aattactaat gatcatctct acgatcatcc tattttccct ctcaatcatc
    16381 ataagtatag cgacttatgt atttaaagat gatgcaagaa ctacgatcga atctaacact
    16441 ttaggaacta ccctaatatt aaccgataac ctaacatcta agttcttagc attatctaag
    16501 aatttaaaat tagttgcaga acatatctca gaagataatg ggaaatctac caatcgagat
    16561 ttcaaaaaaa ttttttttga aaatgatagt gatgttttat ttctaggcgt ttacagacaa
    16621 gaaggagaag aactcattct cactgaaaaa ctttacaatg aaggaagaga aacaaacgcc
    16681 tcgttggaag aaaatgattt tattagcact gcaaatgcaa tgtctagtta cttttcaaaa
    16741 tcttttgcgg gagctactat cctaagaaat gcgagtccaa tttttaaaaa tccagttctt
    16801 ggtttaagta ttccttttaa ggaaataaaa aataaaaaaa caatcatagt cagttttatt
    16861 cgattaaacg gaatattaga atcgtttcaa gcagctggta gttcaaataa ctttctagtg
    16921 gatgatgaag gaaatttact cgctcatcca aatattgatt tcattaaagc aggcaaaaat
    16981 gtttctagtt cgcctatcgt taaaagcatg ctcgaaagca atacaaataa taaataccaa
    17041 cgatttttag atgaggataa tagttattat cttgggtcat ttaagaaatt aggttttgct
    17101 gcaggtggag ttatttccac tgttaaggaa gaaaaagcat tcgaacaaat ctacaaccta
    17161 caaagaagaa atatctattt aatgattatt gtcttaatgt tatctatcct atttgtttat
    17221 ttctatgcaa acagtttaac aaatccaatt cttaagttag tggatgcttc tcatgaagta
    17281 gaaggtggaa attaccatgt aaaacttccg ataggcgcac acgatgaaat tgggatttta
    17341 acaaactcat ttaactctat gacaaaggga ttagatgaaa gagaaaaaat gaaagatgcc
    17401 tttggtaagt ttgtgaataa agatatagca gaacttgcta tgaaaggcga aataaagcta
    17461 ggaggagaaa ggaaatactg tgctattttc ttctccgaca ttagatcgtt tacagcaatc
    17521 tcagaaaaac tagaaccaga agaagtcgta gagtttttga atcaatacat gacggaaatg
    17581 gtaaactgta tcactaaaac caacggaatc gtggataagt ttattgggga tgcgatcatg
    17641 gcaacttggg gcgcgctcag acacgatgac aatgcagtag aaaatgcaat caatggaact
    17701 ctcatgatgc gtgatgcgct tcgaaaattc aacgaaggta gaggtggtga taaaaaacca
    17761 atcattcaaa ttggagctgg gattaacagt ggttatgtga tctcaggaca aatcggttcc
    17821 aatgaacgtt tagaatatac agtgattgga gatgcagtaa acttagcttc tcgaattgaa
    17881 tctttaaaca aaccaatggg aacagatatt ttaatctcag aagaagccta caataaagta
    17941 aaagacattt ttcatgttga gaaaatgcag caaatcaagg taaaaggaaa agaagacccg
    18001 cagacaattt atgcagttct tggacgtaaa gatgatccaa attgcattca gacgttagtt
    18061 gaactgcgta aaataatgaa tatcaaatac gaacctcctg cacctgcacc aaaaggtaaa
    18121 aagaaaaaat ctgacgatga ggaaaaagaa gtaaaatatg aaatcctgga ataaagactg
    18181 gaacattcct gcatttttaa tcttcaatat aattttattt gcaggaatat tttattttga
    18241 tctgcataga agtataaacg taggttcctt tgaaccaatt ggaactgtat cgtttaagat
    18301 aaactccatc ctcagaaaat ttgattcatc tgtagtttgg tattctattc agaccaattc
    18361 tcctcttgca aataaagaca caatcaagac ctatcctgaa tcagatgcta tgatccattt
    18421 gaataatgga acagagatac gtgtagatga agacacgatg atctatctag attatagtgg
    18481 aaataagcct agcatcaact ttgaaggtgg aaatattcaa atcaatagtt ctaattccaa
    18541 agataagaat aatgaaataa acgttaagac gaaagataaa acaattacgt taagcggcgg
    18601 ggatgctaaa ataggatcag gaaatggcga tgatgtaagt gttactgtgg acaaaggaag
    18661 cgcgaccatt gaaggtggag gcgtaaaccg gaaaataaat gaaaatgaaa tcgcaacgat
    18721 cagcaagtct ggtatcagca caagaaaatt gccttactca ctgattgaac cagaaaatca
    18781 aagaaaattc atagcaacaa gcactccatt ttctgttaat ttcaaatggg ctcccacagg
    18841 ttctcataca gaacctacat ttgaattaag tcgtgtaaaa gattttaagc gtgtagatac
    18901 tagattaaaa aatgttactt ctgccaatct aagcttggct gcaggtgttt attactggcg
    18961 tgttggcgga aaaaatgaat caggtcaaat tgaatacagt gatagtaaaa agtttatctt
    19021 aattaaagaa gttggtttaa aattattttc gccagcctct ggagcaaatg ttgcgagctc
    19081 ctccagtgga gcaagagtta ctttttcttg gacacctagc gaaacagtga gtgattatgt
    19141 ggtagatatt tctaatagcc cagatttcgc cccaattctt agatctgttc ctgtaggaac
    19201 taatacaatt agtatgggaa attttacaga aggaacctat tattggcgag ttacaacaaa
    19261 accagccact ggaattccat ccatccgaag taatcctagt tctttttcca taggaactcc
    19321 aaaaccacaa gaagcaaagg tcgtagacaa taaacctatt ttggaagaaa agcaaaagga
    19381 agaaaaacag aaagaagaag caaagaaagt agaagaagct aaaaaagctg aggctaaaaa
    19441 ggtagaagaa tccatagcag ggattgctcc tgagcacctc tccccttctt ctggaaaaat
    19501 tgatataact aaaagcaagc aaattcattt taagtgggat aaagtaaaaa atgcagctta
    19561 ttatattctg aaaattgctc ctgccgaaaa aaaaggaaat actttaattt cggaaagaac
    19621 aagtgcaaac ggctatactt taaaaaactt ttcaaagttt agcgagggaa catttttatg
    19681 ggaagttact ccttttgaca aaaaaaataa ttcaggcaaa acatcccgtg gaaaatttac
    19741 tatagatctg tcagatgacg gtcttaaaaa tctaaaacct gaagaaatta aaatactctc
    19801 tccagatact atttatagag ataaataaat gatgaaaaac aaaaagaata attttaccat
    19861 actgtataaa ataagtttaa ttttaagttt tctttctatt atccttttta gctttgaact
    19921 cttcgcaaaa gatacgggat tcatcgaatg gaaagcaata ccggcggcaa atggttatag
    19981 agttgaaata aagtcagcgg gaaaaattat ttcagaaaca aacgttagtt caaacataca
    20041 ttacgtagat ttgcccaaag gaaattacga attccgaatt ggtgttttga atttttttaa
    20101 aaaacctgta gtctggtctt attggaatcc actcaaagtt atcatttcgc aaattccaat
    20161 ccttgtatcc gaaagagaag aaaaagtaat tggtgaaccc ttagtgatag aaggagacca
    20221 tttcttagaa aataccaagg taagtttaat taaagatgga gtcactacaa atgtagattc
    20281 taaaattgta ggtgaaaagc aaatctcaat tccaacggat aagatgcaag taggaaccta
    20341 tgacttaatt ttagaaaacc caaacaacaa aaagcttaca aaaaaagaat ttttagtttt
    20401 gtataactca aagggtggaa tcagaagaaa ggctcctgaa aaaattataa aaaagaaaga
    20461 cattgaaatt tcaagtgaat cattagaaaa ggcagacgtt agctttgatg aatctgaacc
    20521 tggcaagaca aaaatcaata ttggtccaaa atcaaaagaa aaattagata tcaatctaga
    20581 aaacaaatct aatttggacg ttagtgttga tatcaataat agatcagcag gtaaggtaaa
    20641 tttaaatgtt gaaaataaat caaaagataa gattacttta aatgtaacaa atcattcaaa
    20701 agaaaaaatg attgttgggg tcattgataa atccaaaggt aagattgatg taaacattac
    20761 cccaaaatca aaacagaaag tagacattga tactattttt gcagacattg aagacttcga
    20821 tgtgacgata gaaaacaagg caaaatctaa atttgaaatc aacattggaa agcctgctcc
    20881 tgaagtcatt gttccaaaaa gaactccagc aagaattatc aatcgaaatg atatctctgt
    20941 aagtgctgat tcattggata aagtagatat tgctgtggat gacacaaaac caaataaact
    21001 ctcagtagac ataggaccgc atactgaaga agatctcgaa atcagtctag aaaataaatc
    21061 gaaagaaaat gttgcaatcg atttaaataa caaagcaaag ggtagtgtta agttactagt
    21121 agaaaataaa tcagatgaaa atctagcgat caatgtaaat aacgcatcga aagaggctct
    21181 catggtagag attctagatg cctctattgg aaatgtggat attacaaagg atgaaaagtc
    21241 taaaggtcgt ataaatatta gtcgctccga aaaggttgta gaatactttg atgtaaccat
    21301 tgaaaacaaa acaagggata agtttgatat taatattggt aaaaaagaac ctgacccact
    21361 tgttttcaaa aaatcaaacg aagtttttcc aagagattac tatacctata gtttaaagaa
    21421 ataccaagac tttttaaatg gacttaaacg cacctgttct tccagtttag atattccaga
    21481 tatattaatt gaagaatgtt attcgagaca tgcaacatta aacttggaag acatagatag
    21541 acaagtttta tataatttta ttaaactaaa cagtggaaat tatatttcta gaattggagc
    21601 atataaattc tttatagaaa attgtagccc gcctttagca tttatattag aacttgccga
    21661 ctatcgttta aaaaatttaa ctatggatga aaatgaaatt ttctacatta aaaagatgaa
    21721 agaaagtttt agtagctgta gaagtaaaga tggacaatag ttccaacatt ctcctcgctt
    21781 tcgatttcaa ttttaccatt tagttttgct attaggttct ttgttatcga aagcccaaga
    21841 cctgttccct cctctcccaa agtccctgct ctgtgagagg aaaatttctt agtcattagc
    21901 tcgtttagta ttgactttgg aataccaaca ccgttatccg ctatgcttat atgtaaatat
    21961 tcttttcctt cttttatttc tttctctacg attaaactta ttttaccatt tgtatttgta
    22021 aatttaacag cattgtatag aatattgttt aagatttgat ccactttaga ttcatccagt
    22081 aatacaggaa ttgattcact aaaatatttt acttctaaat gaatagattt tttttccaag
    22141 gatctagcca gcaaatctat ggaatgattg attgatcgaa ccaaatcaaa tttggtcaat
    22201 tcaatttttg caacctctgt cttgattgat tgcatcagtt gattgatcaa ttgaagaatc
    22261 ttttgacttg aatgaaatat agtattagaa tattttaaaa ccttttctga atcgagtgtt
    22321 gtattggatg acatcaaaaa ttcagacatg atcataatag aagtcaaggg tgaacgaata
    22381 tcatgtgcca ttacttgtaa tagattatct ttctctgtat tcagtttttc caattcattt
    22441 atatgcttta atctttcttg ttcgagagcc tttattttta aatgaacctc tattcttgca
    22501 attacttctc tatcatcata gggcttaata atatagtcaa cagctcctct ctctagacct
    22561 tttatctttg aatcaaaatc ggatacagca ctcaaaaata taattggaat atcctttgtt
    22621 ttatcattcg atttaacttt ctcggctaca tcgaatccat tgatttcagg catgatgaca
    22681 tctaaaagaa taatatctgg gacttccgtt tctaaaattt ctaaacattt tctaccacca
    22741 ttagctgtta aaactctata accagcattg agtaaaattt gatttaataa gataacgttt
    22801 agctctgtgt catcaactat tagtattaat gcttgttggt tcattttatt tttttaactg
    22861 gttaagactt tcataaatct ttagagccaa tttatctcca ataccaggga tttcttttaa
    22921 ctcggaaaga tttacatttg ctaacttctt cttccctaaa aaatatttta aaatcgcttt
    22981 ttttctttga attcctagat ccgcaatacc atctaacgca gttttcaatt gtgatttatt
    23041 tcttctttcc cgatgaaaac tcacaccaaa tctatgagct tcatctcgta aatgcctcaa
    23101 aagtctcatt gagggagaat tgatatcaaa agaatatgga tttttgttcc caggaaagta
    23161 gatttcttct ctcttttttg ctagaccaat cattggaaga tcaggcagat ctagtgcgat
    23221 agccgcttcc gtagctcttg ctaattgaac ctctccccca tcgataacga ttagatctgg
    23281 taaactttca ttttcattta ctaatctttg taatcttcta gcaatcactt catgcatcat
    23341 accaggatca ttgattccac tatagctttt cattatatat cttctatatc cagccttgta
    23401 gggcttacca tctacaaaca taacaccact cgcgaccgga tttgttccct gaaaatgact
    23461 gatatcatag cattcaataa ctcttggcaa ttgtttcagc tttaagtttt ctttcaattc
    23521 ttttaatgcg ttagtttggt cttttaattt agtagcaagt aatctttccg ataaattcat
    23581 ttctgcgttc ttggcagcaa gaccaagaag tgcttttttt tctccagcac tgggaaattt
    23641 tagtttaggt ttaaatccta aattcttagc taaaaattcg aataaaactt ctatatcttt
    23701 cttaacactt tgcggaagaa caatactagt tgggataaaa tttgcctgta aataatagag
    23761 ttttagaaaa gatgtaaata cttcttcatc cgaagatagt tttaatccat tcaatgcaaa
    23821 agatttctta ctttccattc ttccatctct tacttctaag attactatct gaccatcatc
    23881 ttcccttttt gcgtaagcta taatatcttc atccccacca gcttggttaa tcactgtttg
    23941 ccgcttctga atgctttgta tattttcaat catatttctg tagatcaatg ctcgttcaaa
    24001 ttctaatctt tctgaatgag cttccattct aattctaagt tcttctagca gagaatcttt
    24061 tttgccttct aagaatttca cgatttgatc tactatgact ctatattctt cttctgaaat
    24121 atttccctga caaggagcaa ggcatctttt tatatgaaag tttaaacaag gtcttctggg
    24181 ttttggaaga ggaagttttt gttcggtttt gcgaattggg aatactttgt gaattgtatc
    24241 tagtaagtct ctagtagctt tcacatctgt atacggaccg aaatactttt tcttatcagc
    24301 tctgattctt cttgtgagat ataccattgg gaaaggctca tctgtagata cacagataaa
    24361 tggatatcgc ttatcgtctt ttaagcgaat attatacttt ggattgtatc ttttaatcag
    24421 attgtcttct aagattaaag cctcttcttc tgtataggtt gcaatccaat ctagatcatc
    24481 aatttcattc tgaagtgcta aagtttttac atcggtgata ttgggattta agtaactgcg
    24541 aactctatct gctagtcgaa cggcttttcc tacatagatt acttctgctt tttcattttt
    24601 ccaaagatag catcccgggg aatccgaaag attcttgatt ttttctttta taatatttag
    24661 tctttccagt tccatacgat tacagagaaa ataggttcat tgcattttta taaaatatgt
    24721 tcttctttgt gactctgctc agtggtgctt ccaatagttt gcgaattgga tgagacaata
    24781 catagggtat atttggaaaa tcggaaccaa ataaaatttt ttccgaatat cgatctaaga
    24841 ttggaagaaa ttcgtctgtg tcctctcctg tcgctaagaa atctacaaac accatggttg
    24901 tatctaattt taaatttgga tatatgtcaa gtaacgctgc ataatctgaa atttcatagg
    24961 cacccatatg cgccacaatt acagaaagat tcttaaattt tttcataaat ctagaaaaat
    25021 attctatgcc tgtaaactct cctggcaaag gagcatgacc tgagtgaatc acaattggaa
    25081 tttgcttctc ttctaaatag taaaaagtat cttctaactc agttcgattc aaatctaatt
    25141 tagaaacttc acagtgcaat ttaaatcctc ttagcccata ctcttcgact gcttttctca
    25201 cataacttaa aacgcctttt tctggataaa aagttccaaa tgggatcgca cctttccaat
    25261 ttttagaatt actaaaaatc caatcattta accattccgc catgttggac ttatgcgcat
    25321 aatttaaagt agtataaaaa taaacccgat tcctttttaa atactctatg catctatcaa
    25381 ctgaatatcg ataagcaatt ccccattgca cagaatcaaa ccatctccat atcagtttca
    25441 taacaatttc aggaaaaaaa tgactgtgta tatcaataat atagggaata tcgtattttt
    25501 ctaatagttc taaatgactt ggatactctc tactctcttc cttgaattct gccttccatg
    25561 gaaaaacggt ttcccctttt ttgattctta aatgaatctc ctctctagtg atatgtttct
    25621 ttttttgtcc taaaggagtg aaggcacaac agggaagaat cttactctgc attgcaaaga
    25681 tccccgaagt ttatttgtgg aaaatcaaaa ctctcttttg ttttatcaaa tccttcaaag
    25741 tgaaatgcaa aatctctaaa atatatattc tctgggaatc gagaagagac ttttacatcc
    25801 tgaaataatt tcggatcatc caagcggatt ctatttttct gatgaggatt taaatcaaat
    25861 ttatattcat ccccaatttc tttaaactga tggacacggt agagttgatc ttggtcaaaa
    25921 gcatctcttt ctatgactcg aatgaaaact tgtttaggag gattctcagt attaaacaaa
    25981 gataaactaa gctggaatgg ttctttacga attagaattg gcttaccaaa tcgatgagag
    26041 agacaacctt caattcgaat aaagtctttt tccttcatat gagaggttgg gaataacacc
    26101 tgtttgcctc taagagactc ttgcacaaga attttatctt ctagattctg aagagttatt
    26161 tttctgttgt aaacatacat tggatctaaa cgaatcttga ataatataaa actaatactt
    26221 acgataagag gaataaataa aatacaaagc gtaaaaaaat tgtatttttc aaaaggagag
    26281 ttctttccgt ttaaagcaat tttatttttt aaatagctga ttctatttcc caaagagagt
    26341 gattcaacta aaaaagaatc ttctagattt gcttttttac gagcttttat tccttccatt
    26401 gctttagatc ttgcatcttc tttttgtaaa cttcctccga ataatccaat aaatgcaaaa
    26461 aaaggaagag caacctcatc atccaaaaga tagcattgaa aaaaacctgc tacaaataga
    26521 accaagattc cagaaaacaa aacggtctga tctgttaaag aattctttaa aaaaagccta
    26581 aaaagataaa accaaaaatg caaaactgtg actaatccga atataccgcc aatcatataa
    26641 aaatgaagag gatcatggtg ggcatgttgc ctaggagtaa taaacaattc ataccaaagt
    26701 tgctcatttt gagaaatcat ttcttctgat tttcttaaat gttctttatc aaatcgaccg
    26761 ggtccgactc ccatccccca atgatctcta atcatagcaa aagtattttt ataaataaaa
    26821 taacgttgat tttctgttgt attgtctttt agaccttctt gaaaggctct ttgcaaaagc
    26881 caattttttt tataaatgga gacgcttaca ataagaatga aaataaaaac tccgaaaaat
    26941 agaaagagaa ttttccaatt ggattgcact ttaccaaaat ctatttgtgt tgtgagttta
    27001 acagcaatta gcccaaacgt aaaaaatagt ccaagccaaa tagaacgact ttgattataa
    27061 aataacacaa tgctaaaaac gagcaataca aaccctaaaa gaagattgaa tccagtttta
    27121 cgatttggaa tcgtgagtaa aaaataagaa gaaattccag gaaagaaaag tccgcaaagt
    27181 cccccaaaag taagatgtgt attcataaga ccaattggga gataagtata ccttccccaa
    27241 agatcccccg caaaatgctg aagcctaaca ccttctttta cttgaaatcc atctgttata
    27301 aattttgcta aacgaaaggg agtaaaaata gagataaatc cagatactat cacgagtcct
    27361 acggaaataa aaaagaagcg aatcattact tttcggtatt ccaaatttcg aatgtgataa
    27421 aaggctggta atacaataaa gcacatccaa aaatcagaaa actcagatct tgtaaataca
    27481 gatgcaattt ccttccattg atactctttt atattccaaa aaaaggacgg caaaagaaaa
    27541 aaataaatcc ccaatgaggc aataaaagct ctatttcgaa gggtaatcat taggcttttg
    27601 tgctgaatag aactatatag aaaagtaaca atcgatagga acgcaaaaga ctgactcaca
    27661 gtcacagaaa gaggaaaaga tataatgaag aggtttaaaa aaatgaccga caatttatct
    27721 aagttcataa acgatttaat acaggtattt tctctttgga taagaaagaa aacaaaaaaa
    27781 taaacttttc tgtagcaatc ataacctaca atgaagaagc gaatattagg gaatgtattg
    27841 agtcagtaaa ttctctagca gatgaaattg tagttttaga ttcaaatagc acagataaaa
    27901 ctctagagat tgcaaagaca tttgaaaaag taaaaattta ttctcaaaag ttcaaaggtc
    27961 atatagaaca aaaaaatgat gctattcttc tctgtcaagg ggaatggatt ctttctctag
    28021 atgcggatga aagagccact cctgaacttt tgaaaaacat tgaatctact cttaataatc
    28081 ctaacgactt aaatgggtat aaagtatctc ggttaacgtt tcatatggga agatacattc
    28141 gacattctgg ttggtatcca ctctatcgat acagattctt tagaaaaggt tttgctaagt
    28201 ggacaggaga aaatccacat gattatatcg aaatcaatgg aaagggagat aagttaaaag
    28261 gggatatcat tcattatagt ttcaaagatc tttcccatca ggtagataca attaataaat
    28321 tttcatctat tgttgccttt actcgatcta aaaaaggaat ctctttttct gtgtttagat
    28381 ctttaataaa acctctttct aaatttttag aaatttatat tttcaaatta ggtttcttag
    28441 atggctaccc tggatttgtt atcgcaatct cctctgccta ctctaccttt ctaaaaaccg
    28501 caaaaatgta tgaaatggaa aaagaattta tcaaacgacc ttccaacttg agaccggatt
    28561 acggaaatga aaattaaaaa tatatttaag aaattttttc ccaaaagaca tctagcggat
    28621 aatccagaga aaccgcaaag accacctaaa agaccaaaag gatttgagag agaactagaa
    28681 gatttaaaat taaaattaac agattttctg cttactctca aagtaaattc aggtctagta
    28741 gtaaaaagaa atatgtattc tcttactaag aatgggcatg gactctatcg attagaggga
    28801 atagaaaaaa caggaagagc tttttctatc attgtaagca cagcaaacca tcttcaatta
    28861 aatgcagagg gaaaagtaac aggactcatt caagttaatg aagcagaatt aaatagagtg
    28921 atccaaaatg aatactcaag ccttaaagca atgtttgata aatttagaaa tttaaattta
    28981 aacgataaag ctctagaaat tttagatgaa aagaataaat tcaactggaa ggaaatcatt
    29041 ctctggaatc gttattggaa agagatgcta tttctacaca tgagcgcaaa tcatatagct
    29101 ctactgatta ttagcttagg ggattctttt aaaaatgtct atttagaagt ggcaacaaag
    29161 aagcaaaaac aaattatttc agatgaattg ttttatttaa accagggtgt aaactcagaa
    29221 gaaatgaatc caaattccaa aaacttaaat ctaatgaatt tcgatattgc aatggaagaa
    29281 ttcaaaaaga gtattcaaaa gattaaaaag aaaatggatt tggaatcgtg aatctaattg
    29341 aaattaaaaa tcttagtata atggattcct ctagaaggaa tattttaaaa gacttttcct
    29401 ttgaacttag aaaagactcc atccatgccg tgattggaga atcaggtagt ggtaaaacat
    29461 cttttgcaaa tactctcttt cacctaacaa agaaagactt aaatttaaaa tatgaatcct
    29521 attccatttt cggaaaagac tattccgact atcaagaatc ggattgggta aaaatgcgag
    29581 gaaagcaaat aactatgatt ccacaaagtc caggaaaatc ctttcatcct tatatcagca
    29641 ttggaagtca aatcctagac tattttaaga acaaagagcc aaatctagcc caaaaggatt
    29701 ccattctatc cttactagaa caggttcaga ttagaaatcc tgaagcagcc tttcactctc
    29761 ttcccaaaaa tctatctggt ggagagaaac aaagaatatt ggtagcaatg gcgattgcgg
    29821 tgaagcccga aattgtagta gcagatgaac caactaccgc acttgactcg atcaatgaaa
    29881 agttgactct aaacctgctc tatactctat tgaaaaacag tggaattgga cttgttctaa
    29941 tcacacatga tatgagaatc gttcgagagt tagcagacga tgtaaccgtt cttagatcgg
    30001 gagaactaat cgaaaaaatc actttactaa ataaaaatat gaataaatta caatctgaat
    30061 attcacaaaa gttggtttca aaatcattgt aatcagagag aaagaggtat agtttgttat
    30121 ttagaaacct tataaaagta tcttctataa aaatcgtttt aggcttttta atgccattag
    30181 gtctcgttgc agattctaaa gttcaatttg aaaaacttga tacgactaga tatccacttg
    30241 ttcacatcta tatgcaagaa aacaaggcaa aacccttaca aggggaaaat attcacatca
    30301 ctgaaacaca ggaaggattt actaggcaag ttcaaacaat caatatccta aaatcaaatc
    30361 aactcagacc cattcgttta attctttcga tccaagcatc caatgaagta gaccgtttac
    30421 aatattccaa acaacttgct acctctttcg ttcaaagttt aggaaaagaa gatagaattg
    30481 gtttacacat ctttgcaagg gatacttatt ttttaaattt aaatatggat aaagcagaag
    30541 cgcttgagaa aatagatgcc atttcgcaag gctctggaaa tagaaccaac tatgcgctaa
    30601 actttctttt atctcaaatc agagaagacg aacttcccaa tctacttttt acaattacta
    30661 cagataaaga agtggaagtg gatgatccaa ttgctagtgt aataaaaaaa tcgcgtggat
    30721 taaagcttcc gattcatatg cttgcgctga gtgaaagaaa gtatttaaac attgctgact
    30781 acagtgaagg ggatttttat cccatcgaaa aaaatacatc gattgcactc atgcaatctc
    30841 agttgtattc ctttcgaaaa atgccaccaa tcatagaata taaatcgccc tttgccgaaa
    30901 acgttaagct atttcgacca acctctattg atcttaaact caaagtggga gaaagtgatt
    30961 ttaaagcaag ctatgaatta actttactca gttttataaa atctaaattt gcaaatattg
    31021 aattctttta ctccttcatg ctagtagttc tacttatttg cattttactt ttatacgcag
    31081 ttacaaaaag agcaagagaa attgcaagaa gagaagaaga gagaagaaga gaagaagaac
    31141 ttttaaaaaa tgatctttat taccatgaaa atatgtcaac cgacacaaca gaaagcccaa
    31201 catttaaaag ttactctagc tacgaagatg aaacagtcat tgatgaaaca gaagatttat
    31261 attcttacaa tgaagactct ttatccgaaa gtcctactca aagagattta gacaatctag
    31321 atgatggata tgatgtttcc cctgatctat tagagtcagg tgaaaaatat gaaagaggta
    31381 ttttaattct aaaagaagga ccaaatcctg gtagacaatt tacaatcaat aaaccagaaa
    31441 tcacaattgg tagcacagcg tctaacgact tagttctttg ggattcttct cttagtccaa
    31501 tgcacgctaa aataaaaaga gtaaaaaaca tatttatttt attcgatact gtctcaaact
    31561 caggagttta tctaaatgga aagaaacttc ttagaccaaa agcactttac gatttcgatg
    31621 aaatccgatt aggaaatagc ttacttttat ttagaggaaa ataatttgca atattcagaa
    31681 aatatcttaa aaaaaacaaa atccgaaagt tatgtaagcc caaaagttaa atgcgtttct
    31741 atcgaaggaa aaggcaaagg actaattgca aaagaaaaaa tctctaaggg agaaattgta
    31801 agtatttctg gtgggttaat ttttaaagaa gaggatcacc aaaaggaatt agtagagaaa
    31861 gggattggag attattgcta ttatatcgaa gaaggatttt tgatttgtcc aatcaatcca
    31921 gaagatccaa gtgatgattg gagaatgaat cattgttgtg aaccaaattg tggagtgaga
    31981 gggcaaattg tttttgtagc acttagagac atcgaaatca atgaagaact aacgtttgac
    32041 tacgcaatga cagaaacaga tccaaattac tcagttccac tgagttgcga taaatctagt
    32101 tgtagaaaaa ctttttccgg taacgattgg aaaaatccag atattcaaaa gaaatacaaa
    32161 ggacacatgt ctttatacat agaaagaaaa attcaaaata atgaaacatg aaatcttaaa
    32221 agacagagtt tttacaattt caaatttttt aactgtttca agagcctttt ttcttccctt
    32281 ttttatagag tatgcctatt tgtatcacct agatacatct attataaaat attactatat
    32341 tctaatctct atctgcggtt atgttgtgtt aagtgattat ttagatggac ttatagcgag
    32401 actcttaaaa gaagaaacaa ttctaggaag atatctagat ccagtttctg ataaaattgt
    32461 aacgattggg ggactttcca ctataacagc ctactttcat tttcctcttt ggattttgat
    32521 tttgtatatt attcgagaaa tcatcggagt ttgggtggga ttatttttat acttcaaaag
    32581 agggttacaa gggaaaccaa atatttgggg caaatgggga gtagccttag tcgcaatttc
    32641 tgtggttcat tatctaagcc tacccttact ccaaaggaat tatccaaatc ttgtttttac
    32701 aaaagagcct gaaatcagcg gatacttact cattgccgtt gtctttatag gatctgtaaa
    32761 atatttaacc gattacaaaa aggatttagt aaaagaataa ttattttttg aaaaagaaaa
    32821 atcatactat acgaatttcc taaacctgtt aaagataaat aaatgctttt tttatcatag
    32881 tgtatcctta aaattaaatc aaagacgagt gtcaaaattt tatgaatcta ataaagaaat
    32941 taaatctctg gaaagatgct gggttaatca gtgatattca gtatgcttcc attataaaat
    33001 acgaagaaga gcacaaaccc aaaaatagaa cggcttatac tattattact ctcggcgcac
    33061 ttgttatttg cctcggtatc atctctctaa tcgcatccaa ttgggagtat ataaatgatg
    33121 gagtcaaatt agtaattgat tttctaattc ttggcggaat atcttattcg atctacattc
    33181 aaagagaaaa taataaaaaa tggcttctag aagtcttaat tatttcctat tttataatga
    33241 tcttagcgtc tatcggtctt atatcacagg tttacaacac gggtggaaaa ctttaccaag
    33301 cttttttatt ctggtgcttg attacactgc ctttagtatt tcagtctagt ggaagagcca
    33361 caacacatat ttggctgatt gctttcttat tttcttccac atctttctta tttgatagaa
    33421 tttcattatt taacaaggat gaagtagccg tggtttggct cttttctttt ctacccactt
    33481 cccttttatt tatttctttt ccacttaaac aaagtaaagt cgatacactg acagtctttg
    33541 gaaagacttg tcttttttgg agtatacttg gatttttatc cggaacaatt gtcttttcct
    33601 ttttaaaaga tatttcaaaa attaaaaatg atagcggttt acttgcaatc cagggcttac
    33661 tgatgattag ccttcttttc tcaattctaa gtgtttacct aagcttaggt caaaataaaa
    33721 agatagctgc ctttttagga attggttcta tactttatct atttatgctc tcttctcatc
    33781 tattttattt tcactctgaa tttttagaca caatcttttt tattttgatt tggtttgtta
    33841 ttggattgct ttttcatctc attgattcaa aacaattgtt tgaatttgcg attgtagtaa
    33901 tagggattcg ctttttggta gcctattttc aattatttgc ctcgcttgca cttactggaa
    33961 ttggacttat attctctgga attctaatta ttgtaatttc tgttctatat attagaaata
    34021 gagataagat tacaaattgg ataggagaat taatatgaat tctaaaaaaa tatttctact
    34081 tgcattactt tttccattta ttgccttatt attactaacg ttttataagg catattcact
    34141 gcaatcaggt cttcgtttta ttctaccaat tagtggttat gatcctatca atccaattgc
    34201 tggtcactat gtaacctatc aggtagaata tggacctatg acttgtgaag gagtagaccc
    34261 taaagcgaaa gaggaaagtt gtatttgtct ctctctagca catgacaatc attacttttt
    34321 accaacctgc gaagaaagag aaataaaaag atgtgatgcc tatctacgag gaaaatgtag
    34381 gtatggtcga tttgaagcag gaattgaaga atattttata ccagaagaaa aagcaaaatc
    34441 gatagacgaa actattcgaa aaggtaaatc aaaaattaag atctccgttc aaaaaaatgg
    34501 aactgccctt gtagaggatt taatattggt agaggattga atttatttaa cgtgcgttct
    34561 atataagaat atttaaccac atgtgagggt gttcgtcgtg aatgattgga gcgaagggag
    34621 tactagacac agatgaacac cgatgagttc aaatctagat gaatcattat gatatggtca
    34681 ttccaatgat agaatttgaa ttttcgaaag tgaaaaatgg tatcaaacca atgaatattc
    34741 ttaatatatc cgtgtatatc tgtggtaatc ttataaatct tcttatatta aaactaacgt
    34801 tacttcaatt catttacaat ttcacgatct ggctcaatcc aagaacgatt agattcaaca
    34861 gtgttcaaag gaataaattc ttgtttttga cgaattagat ttttatttct aaaatataaa
    34921 tttagttttc ctagcatatc aggtgcggga cattttgctg tataaatatt tttcttaaag
    34981 ctaaatgcag aactgagttc ttcatttgta aaaaagtaaa ccggcttttt cacatcaatc
    35041 gcttttaaat aatcataaga tccaccattt ttaaataaaa gttgcacatc aatacttgta
    35101 tcttctacat ccggtagtat atgactaatt ctaaactgaa tcccttctga ctttataatt
    35161 ttttctaaga ctgcatcaaa actattttct ctctctgcaa tcacaggaag ttttaaattt
    35221 ttgagagcag gattcatttc taaataactg agttcgtctt ccccgataaa agctgcatcg
    35281 aatcctgctt tgtttaatag attaaaatgc gcttgcattt tagcaccctt accggaaaaa
    35341 tttccgccag agattaaaaa aacctgacct ct
//
LOCUS       DAOQOS010000051        35119 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_508_length_35119_cov_6.509468, whole genome shotgun sequence.
ACCESSION   DAOQOS010000051 DAOQOS010000000
VERSION     DAOQOS010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 35119)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 35119)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35119
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_508_length_35119_cov_6.509468"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            220..633
                     /locus_tag="PLF66_12635"
     CDS             220..633
                     /locus_tag="PLF66_12635"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HMZ64936.1"
                     /translation="MKFPLEDILILSSAMSFGAGVFFLYSYYVNKKANANLSIAPGTL
                     EEQLVHLGKNYYEIGREENSDTIKINSKKVSRRHAILRKKFGKFYITDVSSTNGTFLN
                     NRKIEPFEEYQLFNEDEIRIGGVMLKFYNLTNQSF"
     gene            889..2742
                     /locus_tag="PLF66_12640"
     CDS             889..2742
                     /locus_tag="PLF66_12640"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HMZ64937.1"
                     /translation="MKYLNKLIENISSASILPDDSLQLRINKKLISIISFFITISGLL
                     WGVMYIALEFYLAAISPILYSVIIGFSLILFRKNKNFDRFVKTQLLMILILPFSLQWS
                     LGGFENSGAVMVWAILGPFGSLIFQENKDAIFCFLLYLLFLLVSILQNLYFPFESIPE
                     SIKILFFGLNISFVSIFSFFTMLHFVNVSKKEHEREALLNQQREIIDSQKKITESYAR
                     FVPMEFLSFLGKDSIEDTMLGDFIDDELTVLFSDIRGFTTLSESMESHELMKFLNSYL
                     SEVSPIIKSNFGIIDKFIGDAIMAIFPQSPLNALYASIELIQKIRSLNESLEQFKINK
                     IHIGIGMHTGKMTVGTIGSDTRMDTTVVGDAVNLASRIEALTKVFHVPILISEQFYKL
                     LDAEDTQYIREIDITSVKGKSQPVKIFEVFGADPPDIIQKKLETKNTLMEAIHLFREG
                     KWNEAKNLFTACQKKCPEDPIPVIYINRCENLKNENKAKIIQSIEDLKQKQKTVLIVD
                     DNSAMLEVLQFKIRKNKYYAITANNGLDAIQKYEQFSPEVILTDLYMPDMNGFDAAKK
                     ILEIANRRKEPVKIIFITSEDSDEIKEEILKNGYSFLPKPINYTELFNQLS"
     gene            2775..3227
                     /locus_tag="PLF66_12645"
     CDS             2775..3227
                     /locus_tag="PLF66_12645"
                     /inference="COORDINATES: protein motif:HMM:NF021538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2269 domain-containing protein"
                     /protein_id="HMZ64938.1"
                     /translation="MHYPLIKLIHILSATLMIGTGFGSAFYLYFTYKKGNVGTVRDVL
                     KLVILADTIFTTPSVFLQLITGILLTEMIGLTFSKWFWEVMGISLIVFVLWVRAAFIQ
                     LRLRKILEQENEITDKFHREMKIWFYLGIPSFSASMLIYYMMVFKVTF"
     gene            3228..4451
                     /locus_tag="PLF66_12650"
     CDS             3228..4451
                     /locus_tag="PLF66_12650"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HMZ64939.1"
                     /translation="MHILILGATGFIGNHIFHSLATKHKVSIAGRQAIGSYPHWHFLD
                     FTKEEDWNSILDGVDLVINCIGSIEGNLTQIQRTGPIHFYKLCTQKKIRILHISAIGA
                     DSTNPKTEFLQTKKETDDFLLNYENAKILYPAIVIGKEGKSTQFFTELASLPIAFLPK
                     NLNPPTIHIRQLTSLVSDVVENFANYPKQIFAVAKEESFLELLKKINPRKRITVNIPL
                     FLPKLVFTIFPKLKIGIFSKDMFTLLEDLPTEKYYNHPANFTNLSFKDPIQEKKTTDL
                     IDPKELIQSQSIPYFLGLISVSFIWIWSAISSLISWDLSNQIMKEIIPNDLISAFSIY
                     LGCFVDIVLGITMFYEKLRSPVLKLQILIIATYTIILSIFAPHYWLHTLGVIGKNIPL
                     LALIYYLLQKENETK"
     gene            4438..4812
                     /locus_tag="PLF66_12655"
     CDS             4438..4812
                     /locus_tag="PLF66_12655"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HMZ64940.1"
                     /translation="MKQNKFSIKKRLESFSYAWNGILLLIKEEHNARIHLFAAFLAIS
                     LGFYFSLSSIEWICLLITIGLVISLEAVNSAIENIADFISPERHKQIKKIKDLSAGAV
                     LIVAIVAFVVGLILFLPKVNLK"
     gene            4850..5527
                     /locus_tag="PLF66_12660"
     CDS             4850..5527
                     /locus_tag="PLF66_12660"
                     /inference="COORDINATES: protein motif:HMM:NF018766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1361 domain-containing protein"
                     /protein_id="HMZ64941.1"
                     /translation="MILKILKDKNRFHETLFMGIISVICFGFSIFRFFYTETKVFLFL
                     NWNLFLAFIPWAVTSIAILKPKLQDYKLTVFILLSVWLLFFPNAPYIFTDLFHLRLNS
                     KMPKWFDLILILSFAWTGLLFGLLSLWDIESILSKFFSSIWIRVISICLLFISSFGIY
                     LGRYLRWNSWDIIQDPFGLLQDIGDRIVNPTDHPKTWGVTLFMGIFLTIVYLSFSIIQ
                     KRNSRNS"
     gene            complement(5530..6159)
                     /locus_tag="PLF66_12665"
     CDS             complement(5530..6159)
                     /locus_tag="PLF66_12665"
                     /inference="COORDINATES: protein motif:HMM:NF013488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
                     /protein_id="HMZ64942.1"
                     /translation="MKVEIWSDIMCPFCYIGKRKFEEALEKYPNKEDVEVVWKSYQLD
                     PSIKYQAGKDIYTYLAEAKGQTVEWSKRMHENVVNMAKSVGLEYNFDKAVVANSFDAH
                     RIIQYAKEKGKGDAIEERLFRAYFTEGKNFSDPETLASLGEEIGLDPKEILEILSKDS
                     YSDQVISDIEEGQSLGLRGVPFFVFNRKVGVSGAQDSSVFLQALDQAGN"
     gene            complement(6596..7186)
                     /locus_tag="PLF66_12670"
     CDS             complement(6596..7186)
                     /locus_tag="PLF66_12670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64943.1"
                     /translation="MKEINEMSIGELAAFVSSHLRENGINATLSGGACVSIYSSNRYQ
                     SYDLDFIENLSSSRQKLKSILSKIGFVEESRYFKNPKTKFFIEFPLGPLTVGDEPVKE
                     VIKIKYETGELQIISPTESIKDRLAAYYHWNDNQCLEQAFLIAEMKEINVAELERWSV
                     NENKADVFSKIKNKFILLQKNLRKLKKKKQNSCSNV"
     gene            complement(7183..7512)
                     /locus_tag="PLF66_12675"
     CDS             complement(7183..7512)
                     /locus_tag="PLF66_12675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64944.1"
                     /translation="MLETLLGSMTREKVLIYLYIREEGYAREINSFYKVDLTPIQRQL
                     EKLEIGSVLFSKRVGKTKLYAFNPRYPFLKELKQLLAKAFEFYPTEIKDSLLLNRRRP
                     RRSGKPL"
     gene            complement(7686..8183)
                     /locus_tag="PLF66_12680"
     CDS             complement(7686..8183)
                     /locus_tag="PLF66_12680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64945.1"
                     /translation="MLIEKFLSFPDAENFPSGVYDLDTISFELDQWRLLEVILHLGWV
                     YDTYLLRIVVDTSVDWELWHDSSAVSHIEQRKVAHNPFFHYIEFSIPYDMQLNVDLEF
                     DKVSGRVVKIHFQPAVPELVFVIAFEFETGSFIGLCAKGWRLFNTTKPDDSWPSDEWK
                     DWNTM"
     gene            complement(8269..8859)
                     /locus_tag="PLF66_12685"
     CDS             complement(8269..8859)
                     /locus_tag="PLF66_12685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012389173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HMZ64946.1"
                     /translation="MPHYGFLRETNALAKKAGIDKDTGLHRTGLEEYLKIIFTDTNDW
                     IHDKALGLVDGKLYRTRPDYRSESLKMIVEFDGLQHYTKPDIIEKDFKLTETYTKLGY
                     TVVRIPYFIQLSNQAVKTLFGIDVEEKLFDEKIPSLGVKGQNTPAYLCPAGLKRMAED
                     FKKFPEQYKVNFVFLKKQNDPFRTGVEFLEKEYNSI"
     gene            complement(8896..9198)
                     /locus_tag="PLF66_12690"
     CDS             complement(8896..9198)
                     /locus_tag="PLF66_12690"
                     /inference="COORDINATES: protein motif:HMM:NF026989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64947.1"
                     /translation="MAVHKNSNENMDDHHLYEIVDSEDNIVFKYGICGKPLNSDGTSK
                     RANEQLTIFNRLVGYAKYIANILITGIPGRVKAKEIENDYIAKFEKKYGHRPRGNP"
     gene            complement(9200..9796)
                     /locus_tag="PLF66_12695"
     CDS             complement(9200..9796)
                     /locus_tag="PLF66_12695"
                     /inference="COORDINATES: protein motif:HMM:NF025390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4240 domain-containing protein"
                     /protein_id="HMZ64948.1"
                     /translation="MISVKGKYENGKIELNQLPKIQSRTNVIVTFLEEDTDVLFNEDY
                     FWDLIDLIDWNQDTDSKILSPLLDRLVSLSVENICQFKEILSEKLFLLDTKEHAKHIG
                     DYAYKGDSSFSPDIFLFTRALVVAKGKDFYTRVLLNPKLMPKNSDFEAILYLPAEAYK
                     FKTSKEMNYVPKYIPETFSNNKGWGDDFDLKKMVFGDK"
     gene            complement(9996..10919)
                     /locus_tag="PLF66_12700"
     CDS             complement(9996..10919)
                     /locus_tag="PLF66_12700"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020763966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HMZ64949.1"
                     /translation="MAIPDFQSIMLPLLKYLKDQKEQSSKDIKDHICKEFKLTQKDLE
                     AKLPSSSSISLISNRVAWAEAHMKMAGIIESPRRGFYKITNEGLNVLKNPPEKINIKF
                     LATFEPYKRKREDWQNKEKEENNSVNENDEVNSLTPDEMLEIGYKKIKNELSIELLNK
                     IKECSPKFFESLVVELLIKMGYGGSLEERGSITGKVGDEGIDGVIKEDKLGLDIIYIQ
                     AKKWEGNIGRPEIQKFVGALQGQRAKKGIFITTSSFNENSYDYVSKIDIKVVLINGEE
                     LANLMIDYNVGVASKRSFEIKKIDTDYFIEE"
     gene            11782..12348
                     /locus_tag="PLF66_12705"
     CDS             11782..12348
                     /locus_tag="PLF66_12705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64950.1"
                     /translation="MKSSQINFYLMPEDWKEVERYVQRNGLMLLVSPSPAEKLRVVSS
                     INSSSNSEFTIHKYYTALKTDEDKIIQKFVKAQNYYTIDESNSPVVELPVPFYQNHLE
                     KLNRGRLYYTKEFYLEKTFAPKENQFLNMANDLFKWFRKTFRTDKLDGYEDFIISPSA
                     FQFHQNGGILLLNPELSTSIPNKEKVLA"
     gene            complement(12398..12667)
                     /locus_tag="PLF66_12710"
     CDS             complement(12398..12667)
                     /locus_tag="PLF66_12710"
                     /inference="COORDINATES: protein motif:HMM:NF014043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HMZ64951.1"
                     /translation="MYTRTYKLYRVLSDQKTFDAVLMNFVVIGETVGKLDEKTLELTN
                     SIPWKKIKSFRNIIAHDYMGVVPEEVWEIIKSDIPDLHEKLKNIL"
     gene            complement(12722..12898)
                     /locus_tag="PLF66_12715"
     CDS             complement(12722..12898)
                     /locus_tag="PLF66_12715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64952.1"
                     /translation="MGTDLIFEFDESANQLFASKERIREIVSQHFGVKVDLCREKYIK
                     PFFKESILKDAIFI"
     gene            12971..13672
                     /locus_tag="PLF66_12720"
     CDS             12971..13672
                     /locus_tag="PLF66_12720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64953.1"
                     /translation="MKLFILLTIFSLQRLFAVEVVKFNGEAFDLKTGKRLYYDHHEEY
                     YEGGKHLYSIIQYKDTNGKVFAKKRIVFQKSPTKADFKLEDFRDGYIEGAEVNGDQIK
                     FLYRKNEKEELKEAMITAPNNAVLDGGFDYFVRENWDMILAGNRKQLNLCAPSQLDCF
                     KFVVYKTGEGKRKEADVVMMKVELNNALLAVFVKPILLVYNKETKKLLQYDGISNINN
                     EDGKSHVVRIIYDNK"
     gene            13681..14463
                     /locus_tag="PLF66_12725"
     CDS             13681..14463
                     /locus_tag="PLF66_12725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015681342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HMZ64954.1"
                     /translation="MNFKREALNLNGGKAEIITIQTNEQNSLTRPTMKELANILKEIQ
                     ADDSYKGAILTSENPKFFSNGLDAETLINTPADELVEAVGGICILFGEMLRFDKPLVT
                     EVTGHAMGGGAVMTMASDYKYMLSTGCRISFTEIGFGLPLPGMFVYKLQQSIHLTKLN
                     EICMEAAAYKGPEAKEVGMIDEIAGTREEIRKLSTKKLESVFRFPLSAVRYTKQNINR
                     QALQDFDIHLKNSSDWLYVPVIRNNMLEAMKALKEKRRPVFQ"
     gene            14614..15081
                     /locus_tag="PLF66_12730"
     CDS             14614..15081
                     /locus_tag="PLF66_12730"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HMZ64955.1"
                     /translation="MNTKYFYQLGLILILILSACATIENNPYESRLADAVYEHNLYSV
                     KYALATGAGINEKKSFYGNHTALMIACKEGNYEIAEWLLENGADPNMTTRDGHTALML
                     AAYNRYPDIVKLLLKHKANVNIVSKRGHTALSETTRTDSEKIKGFLKEAGAHE"
     gene            15074..15481
                     /locus_tag="PLF66_12735"
     CDS             15074..15481
                     /locus_tag="PLF66_12735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004788928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64956.1"
                     /translation="MNSLKKIFLGLVVLFLFHQCTNTNALAACRKKCTENESLCIAAL
                     YDKGNFNNNISACALQQDSCHFTCERDSKLIKISRGHGSSSGFRSGGSGHGSSGHSSG
                     GGGHSSGSGGGSGGHSSGGGHGGGGGIIFNDAF"
     gene            15541..15930
                     /locus_tag="PLF66_12740"
     CDS             15541..15930
                     /locus_tag="PLF66_12740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64957.1"
                     /translation="MNYIRYGFIAAGFTNIAGVLLFSKFFTNPFMNEASPVVMSNFGL
                     LMIIVWGFAYISVSTAYQNVPWLVLLFTVEKLIYTISWILWFKQNDLTFPYTKDLFAG
                     LFYTLYGFNDFLSMLFFGWVFWISRKE"
     gene            16002..17486
                     /locus_tag="PLF66_12745"
     CDS             16002..17486
                     /locus_tag="PLF66_12745"
                     /inference="COORDINATES: protein motif:HMM:NF013595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HMZ64958.1"
                     /translation="MENLGLKKILLSLHCLLLSFCLNAERSPFDMNNQASPIGSLLMF
                     SGSGRTSALPSPTNTAFQYSQTDYTTYTGSTLSISAPSITSGSVSSFSISPTLPNGLA
                     LDTATGSINGVPTGSTSNQTYTITGKGQINDATSTLSIKIGSTSAAGLVGQSSFNFSG
                     GGSGNSSINGLYDISLDSNSNLYIADSNNNRALIFLSGSSSASTVFGQFGSYASIAIN
                     NGGISADSLFSPRGITVDNSGNLFIADTSNNRILYYLPGSTTATRVYGQLGNFTTGTI
                     NNGGISANSLAGPYKPTVDADGNLYIANLFAHRVLYYPAGSTTATRVYGQLGSFTTNT
                     LNNGGISADSLNAPVGTALDANGNLYIADRQNNRVLVYPPGSTTATKVFGQFGSFTTN
                     TANNGGVSANSLSAPTGITIDTNGNVYIADSANHRVLYYPTGTTTATRVFGQMNSFTS
                     NTANNGGITASSLNTPYSVAVDIKNNKLYVADNGNNRVVIFYVP"
     gene            17600..18781
                     /locus_tag="PLF66_12750"
     CDS             17600..18781
                     /locus_tag="PLF66_12750"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010577299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HMZ64959.1"
                     /translation="MEQAVIIDGVRSGFGSFGGSLKDFGATEMGVEVAKAAIAKSGVK
                     AEDIGESIFGNVVPSGKDSIYLARHIGLKAGLKVETPALTVNRLCGSGMEAIVLAAKK
                     IYLNETNVVLAGGSESMSQAPYVVRNARWGIKYGPTEFEDSLNQGLTDLYVELPMGMT
                     AENLAVQYSISRQEQDEWAALSQERAEKATLDGILKEEITPITLPGKTPIVFDKDEFI
                     RGKASRDKMAGLKPAFKKDGTVTAGNASGINDGAGAVIVSSKSYADKLGKKPLAIIKS
                     YGHSGCDPAKMGIGPALAIPRALSSAGLSLKDIGLIEVNEAFAAQYLAVQKELGLNPE
                     ITNVNGGAIAIGHPLGASGTRVTLTLAYEMRRRKVKYGVASLCIGGGQGIAIVLENPN
                     L"
     gene            complement(18778..19128)
                     /locus_tag="PLF66_12755"
     CDS             complement(18778..19128)
                     /locus_tag="PLF66_12755"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HMZ64960.1"
                     /translation="MKTEIRKSDRIFPKDFADYAVHLNAAGESFSGYLGNISETGLCA
                     IMPASFNVEVNAMLEGSVLHWPTSDNMDISGRVAWKTEMEFQKKPHVMIGMEFSEKFI
                     FPEYLIALSLSISD"
     gene            19449..20453
                     /gene="dusB"
                     /locus_tag="PLF66_12760"
     CDS             19449..20453
                     /gene="dusB"
                     /locus_tag="PLF66_12760"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015677258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HMZ64961.1"
                     /translation="MIKIGNVEIPNNIVLSPMAGVSDSPYRQICREMGSGFAYTEFVS
                     TDGISHHSKKSIDLFRFQKMERPISFQIFGNNLEIITEACRIIEDLGPDIIDLNMGCS
                     VAKVSHRGSGAGLLRKPEYVGKIIESMTKAVKVPITAKIRIGWDHASLNYKEIVHVLQ
                     ESGVQAISVHGRTKSMAYKGLADWNVIAEIKSFAKVPIFGNGDIQSYAQAKERIKTSG
                     VDGVLIGRAAIGNPWIFAGIDKSTVSFPEVRAMIFRHLSLMTDFYGEQMGLVLFRKHV
                     SKYLRNYFGIADLRHKLVTTNSLNEFHDLLNNFEIKEFKMVSEEENENLNCETYASAE
                     "
     gene            20663..20788
                     /locus_tag="PLF66_12765"
     CDS             20663..20788
                     /locus_tag="PLF66_12765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64962.1"
                     /translation="MFRPKEETLSCVPPYAGAGFATEQIGGIAATAKRYRAISLS"
     gene            21081..21986
                     /locus_tag="PLF66_12770"
     CDS             21081..21986
                     /locus_tag="PLF66_12770"
                     /inference="COORDINATES: protein motif:HMM:NF025224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HMZ64963.1"
                     /translation="MKQFYVIFILIFISSNNLLAQANCEKFESLKKAFMDNKKESVCE
                     LDLRNQHLKSLPTNMGDLINLEILHLENNKLSELPESLVYAKKLVRVHLDNNQFRKFP
                     EVLLDLDQIVRISMNQNELTEIPTKIENLKELNYLSFMGNRIESLPKQIGKLKKLTYL
                     NLISNDLKELPQMFYNLHHLENLYLGNNDSLKISDEIYKMKNLIRLSISGIGLENLST
                     DIKNLKNLKWLSISSNKIETLPDEICELKDLEAFFFGYNKITKLPHRFEELKKLKKIS
                     LVDLDLGITVENDLRNTFPEAEIEF"
     gene            complement(21988..22992)
                     /locus_tag="PLF66_12775"
     CDS             complement(21988..22992)
                     /locus_tag="PLF66_12775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020776807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HMZ64964.1"
                     /translation="MTSIPETGKAIELINYDGLESSLRVAQRKIGSLKETDVLVKIYA
                     SSINPSDLMFIRGLYGIKKKIPCGGGFEGSGTIVAIGSAVTRVKVGDRVACSAHYMGD
                     GSWAEYMVTPEYNCIPLIEKVTLEQGATFFVNPLTACGLTNIAIKEKRGGIVQTAAAS
                     ALGKMIQRYAKRKGLPVINIVRKDDQVELLKSLGSEHVLNSTSPDYEKNFTRLVKKLE
                     ITYAIDAVAGKTGEDVLNMMPGNSKLVVYGALSEDPIGVSAGSLIFQRKKVEGFWLSY
                     WIADTPKEEFAEIVRDAQENLSTDFKSEINKRFSLDDGYKAIEFYKNNMTSGKVLFVA
                     "
     gene            complement(23119..23883)
                     /locus_tag="PLF66_12780"
     CDS             complement(23119..23883)
                     /locus_tag="PLF66_12780"
                     /inference="COORDINATES: protein motif:HMM:NF024778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HMZ64965.1"
                     /translation="MNYEIIFISSFIYGFSSSFHCITMCGPFVGMLNIIGESKLTTNV
                     IYNLGRFTSYTLIGSLMGFLGFSLNLSSEVTKIQNISIILSTIFILATGIALIFNKKL
                     IPDSGANKIIKKLFHPFMEAMRKSTHTLLFSFIFGMFTGLLPCTILYPAFAMSLATGN
                     PMLGGISMSSFFLGTLPGLLLFGLGFHKIKSYVLKDYAALVGIGIITVGLSTVFLRMN
                     HNHANHHHMGGSSLNQESDKEVDKAEETESQHHHHH"
     gene            complement(23880..24035)
                     /gene="ccoS"
                     /locus_tag="PLF66_12785"
     CDS             complement(23880..24035)
                     /gene="ccoS"
                     /locus_tag="PLF66_12785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004460308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HMZ64966.1"
                     /translation="MEALLITIAIAFIIAFIGLIVFLISIKNGQFEDIEAPKYRILFE
                     DEKESWR"
     gene            complement(24037..26541)
                     /locus_tag="PLF66_12790"
     CDS             complement(24037..26541)
                     /locus_tag="PLF66_12790"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HMZ64967.1"
                     /translation="MVLSKEFESLNKNTKSQKMSSIPTTCYHCQNPIPKNLIVTSKLQ
                     GEDRYYCCTGCQTVSELISEMGKDFFYDLRGSSNLEPVAEYRKEEAENLDSEVTYSEF
                     VTSVNKTDSEVYINITNIHCSACVWLNEKVLSETKGIKKVRINFSTGRAHIVWDDKIL
                     KLSQIFSIIQSIGYLPKLYAPWKKEKAYNNYATDLLIRMVVAAFSFGSIMAFTVSLYA
                     GYFTGIELGFKRLFHFYSWLLATPVYLYSGVPFFRGAYYGLKNKTLNMDFLLILGISL
                     AYFYSVYVTLTDKGEVYFDSVCTIFFFILIGKYLEAVSRNMANRKINSLLSKLPELCT
                     IIENGVEQKIPSNRVKKDDLILVKAGERLSVDGVLESDHGHVDESFLTGESKPVSKKK
                     GEKIYAGSLSITNAIYLRVINSTQNSTLSILQKMIEDALLEKPAIQRKTDRIATKFIS
                     VVLFVAVSTFIGWMLYSSDFERSLIYSIAVLIVACPCALGLAIPSTLVINNIINSEKG
                     IILKNLDIIEPLSKLDTLIFDKTGTLTEGTLSVIEDTILDPDLNQKLIYSLEKKSTHP
                     IAKSIVNAYSNPSSINDAFVDVKEVEEIQGYGIRGILLYGNKEYRVSIGNLDFIQKYV
                     SLENIKIEDTNLGTIVYVSVNEKYIGLIRLNDIPRVGIKEEILKLKNRVKDIRLLSGD
                     NNKNVTRIAKELGIENYKGELKPEEKLQALNDLQASKKIVGMVGDGINDAGILAKSNV
                     GISLELASDISIDKSDIILMKNDLKGVRLSIEYARLTSRAIKQNIGISFMYNSIMLPL
                     AAFGFMQPVYCALFMTLSSLTVVANSFLLRLFARKV"
     gene            complement(26544..27056)
                     /locus_tag="PLF66_12795"
     CDS             complement(26544..27056)
                     /locus_tag="PLF66_12795"
                     /inference="COORDINATES: protein motif:HMM:NF017559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HMZ64968.1"
                     /translation="MIQVSNNIKSESGSIRFAFIVMTLAFTALIAATWYTVKIAMAGH
                     EPVMDKNYYEKGMNYEKEIAQTVQMRSEGYHFRTTLLEKDSEVTLGNNQLSIGIFKNE
                     EPVKGAKLVLTRERSATNKFTEKKEFIYDKDGMYKANVDFPLDGPWQVTLNASVDGRS
                     FEKTFMIVVK"
     gene            complement(27037..28362)
                     /gene="ccoG"
                     /locus_tag="PLF66_12800"
     CDS             complement(27037..28362)
                     /gene="ccoG"
                     /locus_tag="PLF66_12800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004465025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HMZ64969.1"
                     /translation="MIISRHITGAIRSRRYVVEALLGLIYVIIPWLSWGDKPFMRLDI
                     PARKFHLLGGIYIPQEGIFLHLFLLTAGLSLFFFTSLIGRVWCGWACPQTVFTDFFDV
                     IGRFILGNKYGKKDANPYGKALLYAVWIAFSIFSAFHIVAYFNDPKEMLSRIMSLDFK
                     DVKFQYFIAFFSATLFIDMTVVREQFCKYACPYARFQTVMMDADSYNVTYDYKRGEPR
                     RNKQVKIGDCTACNMCLVVCPTGIDIRDGLNVGCIACGKCIDACTIQMGKENKKSLIN
                     YDSLNRVEKNQKIRWIRPRTVIYATLLFAVLTTAIVLLSDRVPLYASIIPERSLEPMI
                     IPDQKVRNFYILNLQNMTYENRELKLEVVDNERLKPVIHTGTEDSKVLVPANGTAKLR
                     IFIESGNLPKSEKAASIVQVKLQLQDTSNTSYKINKTIPLRLPDDPSIQ"
     gene            complement(28373..28900)
                     /locus_tag="PLF66_12805"
     CDS             complement(28373..28900)
                     /locus_tag="PLF66_12805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002975696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HMZ64970.1"
                     /translation="MDHNKEFDGIKQADNPMPEWWKLFFGLAIVFAVFYTIYFHWFSE
                     WKMADSFNKEVAEYEKQFPMAKVVVSADGSNPMRENADAIAEGKKNFQNFCVACHGPE
                     AKGLVGPNLTDAEWIHGDTDAEIYNVIMEGVGVDKAKLGRGAMPPHKASLGSERVYQV
                     MAWLASNNPSLKKVK"
     gene            complement(28903..29046)
                     /locus_tag="PLF66_12810"
     CDS             complement(28903..29046)
                     /locus_tag="PLF66_12810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016544811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit 3"
                     /protein_id="HMZ64971.1"
                     /translation="MMTALVVYKALRLPVLVIAIMFITFYLYSKKRRELVETPKYRML
                     DED"
     gene            complement(29067..29750)
                     /locus_tag="PLF66_12815"
     CDS             complement(29067..29750)
                     /locus_tag="PLF66_12815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002151300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit II"
                     /protein_id="HMZ64972.1"
                     /translation="MMQKIFDWFSDIADHWDLNGVKFAIYTTIAILIGGLFELIPPFF
                     LTQTVVPIASVKPYSPIELAGRDIYIAEGCNSCHTQMIRPFKWEVDRFDPKKVYGKDG
                     YSKGGEYVYDHPFLWGSKRTGPDLAHESQIQDSVDWHKRHLMNPRETSPGSTMPSYAW
                     LFDAGATINPANIKSHMQGLSKVGVPYSQADYDLVESQLAGKTKGDALIAYLLKLGRD
                     TANLQETAK"
     gene            complement(29761..31218)
                     /gene="ccoN"
                     /locus_tag="PLF66_12820"
     CDS             complement(29761..31218)
                     /gene="ccoN"
                     /locus_tag="PLF66_12820"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010574627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit I"
                     /protein_id="HMZ64973.1"
                     /translation="MNTNTSAGSGSASAGSDPKYNDFIVKAFILSAFLWGFAAMAIGV
                     LIAFQLVYPQLNYTEYFSFGRLRPLHTNAAIFGFALSAIFATAYHSAQRLLRVRIWSD
                     QLAMIHLVLYNVTILLAAITLPLGLTQGKEYAELEWPLDLLIVIWYVIFFVNYLMTII
                     KREEKQLYVAIWFYVASFVTVPILFIVNNLAIPVGLMKSYSIYSGVYDANIQWWYGHN
                     AVAFVLTTPFLGLMYYYFPKHIKQPIYSHKLSIIHFWSLVFLYIWAGPHHLLYSPIPE
                     WLQTTGMVFSVMLWMPSWGGMLNGFLTLTQAKDKIKTDATLKMMLVGITFYGMSTFEG
                     PLLSIRAISALAHNTDWIIGHVHSGTLGWVGFMSAAAFYYLVPRMWNTNLYSEKLANL
                     HFWLGTLGILLYIVSMWVSGITEGAMWRAITDKGFLQYPNWVEITALLKPYRIMRGVG
                     GGMFFIGYVIMIYNLVKTIGTAGSGFKEIDLRVKA"
     gene            31419..32117
                     /locus_tag="PLF66_12825"
     CDS             31419..32117
                     /locus_tag="PLF66_12825"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HMZ64974.1"
                     /translation="MENRINCSSCSSKEYGVFKCASPTALDKIYGDKSHLQFKKKDII
                     FKEHEKMNGFYCIQTGVVRTYKTSASGKEQTFQIAGRGKWLGFRDLISGEESNHSSIC
                     LEDVEVCFIPKETITHLIKSDADFQIEVMKYLADEWKDMENHVHSMGTKQIHSRLAEL
                     LITFHGASGGTENELNINVTREVMATCIGTTTETLIRALADFKDRNWIRVEKNKIELI
                     NLKALYNISEATTN"
     gene            complement(32139..32930)
                     /locus_tag="PLF66_12830"
     CDS             complement(32139..32930)
                     /locus_tag="PLF66_12830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64975.1"
                     /translation="MILRIIIFIFFLNLFSGGILSKEEKVIHVFVSLCDNKNQGIVPV
                     PEKLGNGEDLENNLYWGARYGVKSYFKTQKDWKIISSSLNLNDFILERIVFWNPTKNA
                     YLIADAYKGSAIKKSIENLILSAKQIHLDSVLIYNPFLKKEILLQLAANADLLAFVGH
                     NGLMDFSLELPLKKINNYKSIPIILLACYSKSYFSSQFNSSEISPILWTNGLLAPEAY
                     VLNAGVLSWLENKNASQIRDDAARAYQIYQKCGLKGARRLFSSGW"
     gene            33608..>35119
                     /locus_tag="PLF66_12835"
     CDS             33608..>35119
                     /locus_tag="PLF66_12835"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: protein motif:HMM:NF004531.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HMZ64976.1"
                     /translation="MNKWVYSFDELNQAEEKMGGDWDKVRGLLGGKGANLADMTRAQI
                     PVPPGFTVTTEACNAYIANDGHFVEGIWEQEMDALRALEARTGKKFGDPSNPLLVSCR
                     SGAKFSMPGMMDTVLDIGLNDTTVEGMATLSGNVSFAYDSYRRLIQMFGSVVIGIPDD
                     AFEEVLKEAREEAEVESDSDLDAESLKKIIQKYKSVFKQKAWREFPQDPIEQIRLATE
                     AVFKSWNGKRAIDYRNAEKIPHDLGTAVNIVAMVFGNMGNTSATGVVTTRNISTGDRE
                     LEGDYLINAQGEDVVAGIRATKPISMLKREMSDSYAELLTICKLLEKHYKDVQDIEFT
                     IERGKLWILQTRDAKRTAQAAVRIAVEMAEEWIINKEQAMLRVTPAQIDFFLHPQFKA
                     EEKEKAEHTGQLLARGLNVSPGAATGVIAFDADLAVKWAAEGKNVIMVRPETKPDDVH
                     GMLAAKGILTSRGGRTSHAALVARQFGKPAIVGVNELVRLCQVAHVCKIELKWS"
BASE COUNT        11732 a         6315 c         5736 g        11336 t
ORIGIN      
        1 gattgctccg ctggtacctg aaaccagtgc gtctaccaat tccgccactt cggctattga
       61 aactggaatc tatcatttaa ttgcaagtgt tttagtcatt ccatttttcc ctaaaatgcc
      121 ctcattcaga aagaagatag gttgctgttt ttattgctaa gtcaaaatat ttctggaaat
      181 agagttgaaa aataaaattg atccattact tttaccgcca tgaaatttcc tttagaagat
      241 atcttgattt tatcgtctgc aatgtctttt ggtgccggag tatttttcct ctattcctat
      301 tatgtaaata aaaaagcaaa tgcgaatcta tcaattgctc caggaacttt agaagaacaa
      361 ttggttcatc tcggtaaaaa ctattatgaa attggtcggg aagagaattc agacacaatc
      421 aaaatcaact ctaaaaaagt aagcagaaga cacgcaatac tcagaaaaaa gtttggaaag
      481 ttttatataa cagacgttag ttccacaaat ggaacttttt taaataatcg aaaaatagaa
      541 ccttttgaag aataccaatt gtttaatgaa gatgaaatcc gtattggtgg agttatgtta
      601 aaattttata atcttacaaa ccaaagcttt tagattgtat agaaaaactt gtctctattt
      661 tttagatcca gaatcggtag tgaataaaga gtcatatatt ccaagatttg atgcatttta
      721 aatacatctt ttatcagatt aaatcaaaat attttttcaa aatagcaagc ggataaacaa
      781 aattacctct ttctattttt taatcctttt acctactttc ttttataaaa aacttgtaaa
      841 cttctttgcc agctgaaaac attcgtaaaa aggattcttt tttagtaaat gaaatattta
      901 aataaattaa ttgaaaatat tagttctgcc tcaatacttc ctgatgattc tcttcaactt
      961 agaataaata agaagttaat cagtattatc tccttcttta ttacgatcag tggtttgcta
     1021 tggggtgtga tgtatatcgc tctagaattc tatcttgcgg ctatttctcc tatattatat
     1081 tcagtcatca ttggattttc cttaattcta tttcgaaaga ataaaaattt tgatagattt
     1141 gtaaaaactc aacttctaat gattctaatt ctaccctttt cccttcaatg gtcattgggt
     1201 ggatttgaaa attcaggagc cgttatggta tgggcaatac taggtccttt cggttccctt
     1261 atcttccaag agaataaaga tgctattttt tgtttcttac tttatttatt attcctttta
     1321 gtttctattc ttcaaaattt atattttcct tttgaaagta tcccagagag cattaaaatt
     1381 cttttctttg gactcaatat atcctttgtt tctatttttt ctttttttac gatgcttcac
     1441 tttgttaacg tttccaaaaa agaacatgag agagaagctc tgctaaacca gcaaagagag
     1501 atcattgact cacaaaaaaa aataacagaa tcatacgctc gttttgttcc aatggaattt
     1561 ttatcttttt taggtaaaga ctccatagaa gacaccatgc taggagactt tatagatgat
     1621 gagttaaccg ttttgttttc cgatattaga gggtttacaa ctctatctga atcgatggaa
     1681 tcccatgaat taatgaaatt cctaaattct tatctctccg aagtatctcc aatcattaaa
     1741 tctaattttg gaattattga taaattcatt ggcgatgcaa tcatggcaat tttcccacag
     1801 tctccgctaa atgctttata tgcgtctatt gaattaattc aaaagattcg aagtttaaat
     1861 gagtcgcttg agcaatttaa aataaataaa attcatattg ggattggaat gcatacggga
     1921 aaaatgactg ttggaacgat aggttccgat acaagaatgg atacaacggt tgtaggcgac
     1981 gcagtcaacc ttgcttccag aatagaagca ttaacaaaag tttttcatgt tccaatttta
     2041 atatcagaac aattttataa actactagat gcagaggata cacaatacat aagagaaata
     2101 gatatcactt ctgtgaaagg taaatcacag cctgttaaaa tatttgaagt gttcggtgca
     2161 gatccaccag atataattca aaagaaactg gaaacaaaaa atactttaat ggaagcaatt
     2221 catcttttta gagagggaaa atggaatgaa gctaaaaatc tttttactgc ctgtcaaaaa
     2281 aaatgcccag aagatccaat tcctgtgatt tatatcaatc gatgtgaaaa tctgaagaat
     2341 gagaacaagg caaaaattat tcaatccatc gaggatctaa agcaaaaaca aaaaacagtt
     2401 ttgattgtag atgacaattc agccatgctc gaagttctac aattcaaaat taggaaaaat
     2461 aaatactatg caatcacagc aaataacgga ctagatgcga ttcagaaata cgaacaattt
     2521 tctcctgaag ttattttgac tgatttatat atgccagata tgaatggctt tgatgcagca
     2581 aagaaaattt tagaaattgc aaacagaaga aaagaaccag ttaaaattat atttatcaca
     2641 agtgaagatt cagatgaaat aaaagaagaa attctaaaaa atggctattc ttttttacct
     2701 aaaccaataa actatacaga attgtttaat caactatcgt agaattgaat agtcattaaa
     2761 atcgaaaaac taacatgcat tatccgctta ttaaacttat tcatatactc agtgcgacgc
     2821 tcatgattgg aactggattt ggaagtgctt tctatttata tttcacatac aaaaaaggaa
     2881 atgtgggaac cgttcgtgat gtattaaaat tagtaatact agccgataca atctttacaa
     2941 cgccttctgt gttcttgcag ctgattacag gtattttact tacagaaatg atcggattaa
     3001 catttagtaa atggttttgg gaggtaatgg gtattagcct catcgtattc gtgctttggg
     3061 taagagctgc cttcattcaa ctcagactta gaaaaatttt agaacaggaa aatgaaatta
     3121 cagataaatt tcatagagaa atgaaaatat ggttctattt aggaattcct tctttctcgg
     3181 cttctatgct aatttattat atgatggttt ttaaggtaac attttaaatg catattttaa
     3241 ttctaggcgc aactggtttt attggaaatc atatctttca ttctcttgct acaaagcata
     3301 aagtgagtat tgcaggaaga caagcgatag gtagttatcc tcactggcat tttctagatt
     3361 ttacaaaaga agaagattgg aactctattc tagatggagt ggatcttgta atcaattgca
     3421 ttggtagtat tgaaggaaat ctgactcaga ttcaaaggac aggtcctatt catttttata
     3481 aactctgcac tcagaaaaaa atacgaattc ttcacatatc tgctatcgga gcagattcta
     3541 caaatccaaa aacagaattt ctacaaacaa aaaaagaaac agatgatttt cttctgaact
     3601 atgaaaatgc aaagattctc tatccagcaa tagtaattgg aaaagaggga aaaagcactc
     3661 agttctttac agagcttgcg tctcttccga ttgcctttct tccaaagaat ttaaatcctc
     3721 caacgattca cattcgtcaa cttacgtctc ttgtctcaga tgtagtagag aattttgcaa
     3781 actacccaaa gcaaattttt gcagtcgcaa aagaagaatc ctttctagaa ctgttaaaaa
     3841 agatcaatcc tagaaagcgg ataacagtga atatccctct atttctaccc aagctagtat
     3901 ttacaatctt tcctaaatta aaaattggaa ttttttctaa agatatgttt acattgctag
     3961 aagatcttcc aacagaaaaa tactacaatc atcctgctaa ttttacaaac ctgtctttta
     4021 aagatccaat tcaagaaaag aaaacgacgg atttaattga cccaaaagaa ctgattcaat
     4081 cccaatctat tccttatttt ttagggctaa tcagtgttag cttcatttgg atttggtctg
     4141 cgattagctc tcttatttct tgggatctaa gcaatcaaat catgaaagaa atcattccaa
     4201 atgatttaat ctccgctttt tcgatctact tgggatgctt tgttgatatt gttttaggaa
     4261 tcacaatgtt ctatgaaaaa ctaagaagtc cagttttaaa actccaaatt ttaatcattg
     4321 caacctatac aattatactt tctatctttg caccgcatta ttggcttcat acactgggtg
     4381 ttataggaaa gaatattcca cttctagcac tcatctacta tctattacaa aaagaaaatg
     4441 aaacaaaata aattctctat caaaaaaagg ctagaaagct tttcctatgc ttggaatggg
     4501 attcttcttt taatcaaaga agagcataac gcacggattc atttatttgc agcttttctt
     4561 gcgatctctc ttggttttta cttttccctt tcttcgatag agtggatttg tttacttatt
     4621 acaattgggc ttgtaatttc actcgaggca gtaaattctg ctattgagaa tatagccgac
     4681 ttcatttctc cagaacgtca taaacagatc aaaaaaataa aagatctttc cgctggagcg
     4741 gttttaattg tagcgattgt cgcctttgta gttggtttaa tcctctttct cccaaaggtg
     4801 aatctgaaat aacgaacatg acgcaaaatt gatagcataa ggaataaaga tgattctaaa
     4861 aatattaaaa gacaaaaacc gatttcacga aactcttttt atggggataa tatccgttat
     4921 ctgctttggc ttttcgatct ttcgattttt ctatacagag acaaaggttt ttctatttct
     4981 aaattggaat ctatttcttg cttttattcc ctgggctgta acaagcattg caattctaaa
     5041 acctaaacta caagactata agctaacagt ctttatcctc ttatctgtat ggctcctttt
     5101 ctttccgaat gcaccgtata tatttacaga tttatttcat cttagactca attctaaaat
     5161 gccaaagtgg tttgatttaa ttttaatcct ttcttttgca tggacgggtt tactctttgg
     5221 tcttttaagt ctttgggata tagaaagtat tttaagtaaa ttttttagtt ctatctggat
     5281 tcgagtgata tcgatttgtc ttttatttat cagtagtttt ggaatttact taggaagata
     5341 tttaagatgg aatagttggg atattatcca agatcctttc ggacttttac aagacatagg
     5401 agaccggatt gtaaacccaa ccgatcatcc taagacttgg ggagtaacgc tttttatggg
     5461 aattttttta accattgttt atttaagttt ttcaattatc caaaaaagaa attctcgcaa
     5521 tagctaaaat cagtttcctg cttggtcgag tgcttgtaaa aatacagaag aatcttgtgc
     5581 gccagagact ccaaccttac ggttgaatac aaaaaaagga acacctctca gtccaaggct
     5641 ttggccttct tcgatatctg aaattacctg gtcagagtaa gaatcttttg aaagtatttc
     5701 taaaatttcc tttggatcaa gaccaatctc ttcgccgaga gaagcaagtg tttctggatc
     5761 ggaaaaattt tttccttctg taaagtaagc acgaaacaat ctttcttcga ttgcatctcc
     5821 ctttcctttc tctttcgcat attgtataat tctatgcgcg tcaaaagagt tagcaactac
     5881 cgctttatca aaattgtatt ctaaaccaac agactttgcc atattcacaa cattttcatg
     5941 cattcgcttt gaccattcaa ctgtctgccc ttttgcttct gcaagatagg tataaatatc
     6001 tttacctgct tggtatttta tacttggatc taattgataa cttttccaaa caacttctac
     6061 atcttcttta ttgggatatt tttcaagtgc ctcttcaaat tttcttttcc ctatatagca
     6121 aaaaggacac atgatatccg accagatttc tactttcatg aataaatgat ttcttatccc
     6181 tgactcccga caagtgtttt ttaatgggta gggagtattt taaagattac caccgatcgc
     6241 ttcgctaaca ctgatgaaca ccgatgttcc ctatttatcg tttgcatcat agttttgttg
     6301 ttttatcatt ctttacaagc ggctaatagc aataacacac agattcatca ttttgacatt
     6361 ttgtatttcg gttgtttagt tttaattcaa cttatagacc acttttcata aataagattc
     6421 gctatttttc acaaaggtgg tattcccaat tgccaaaaca gcatattcga tttcaaatca
     6481 gaatttactt ttggcaattg gtatagtttt gagaaataaa tagtaagatt gtttctgaat
     6541 ctggaatctg atatatctat tttattccta tctattcgtt agtcggtatc tctatttaaa
     6601 catttgaaca actattttgt tttttctttt ttaatttccg aagatttttt tgcaatagaa
     6661 taaatttatt tttaatcttt gaaaaaacat ctgctttatt ctcattcaca gaccatcttt
     6721 ctaactcagc tacattaatt tctttcattt ctgcaatcag gaaagcttgt tctaagcatt
     6781 gattatcatt ccaatgataa taagcagcaa gtcgatcctt aatggattcg gtaggtgaga
     6841 taatttgcaa ctctcctgtt tcatatttta tttttatcac ttcttttaca ggctcatcac
     6901 cgacagtcaa tggaccaagt gggaattcga taaagaattt agttttagga tttttaaaat
     6961 atctactttc ctctacaaat ccaatcttag ataatatact tttaagcttt tgtctgctag
     7021 atgaaagatt ttctataaaa tcaaggtcat aagattgata tctatttgag gaataaattg
     7081 aaacacatgc tccaccagaa agtgttgcat tgattccatt ctctcttaga tgcgaggaga
     7141 caaatgcagc caattcgcca attgacattt cattaatttc tttcatagag gctttccgct
     7201 tcgacgaggt ctgcgtcgat ttaaaagtaa actatctttt atttcagttg ggtaaaattc
     7261 aaaagccttt gcaagaagtt gctttaactc tttcaaaaac ggataacgag ggttaaaggc
     7321 atacagtttt gttttaccaa ctcttttcga aaacagaacg cttccaattt caagtttttc
     7381 caattgtcgc tgaataggtg tcaaatcaac tttgtaaaag ctattaatct cacgagcata
     7441 accttcttct cgaatataca aatagattaa tactttttcc ctcgtcatag aaccaagcag
     7501 tgtttctaac atttgaactg acctcattta tagttcaaat gattaactta tgaggtcaat
     7561 caataaatta aataatttga tgcaactctt ttagatttgc gtcattctac taatgagaag
     7621 tattatatta ttacagcaaa aagtttttct acagaacgaa tcctataaag tttatctgtt
     7681 caaaattaca ttgtattcca atctttccat tcgtcacttg gccaagaatc atcaggttta
     7741 gtagtattaa aaagtctcca tccttttgca cacaaaccaa tgaaggaacc ggtttcaaat
     7801 tcaaatgcga taacaaaaac aagttcagga acagctggtt gaaagtgaat cttaaccacc
     7861 ctaccgctca ctttatcaaa ttcaagatca acattcaact gcatatcata aggaatagaa
     7921 aattcaatgt agtgaaagaa aggattgtgc gcaacttttc tttgttctat atgactaaca
     7981 gcggaactat cgtgccaaag ttcccaatca actgacgtat caacaacgat acgaagaagg
     8041 tatgtatcat atacccaacc caaatgaaga ataacttcca atagtctcca ctgatctagc
     8101 tcgaacgata tagtatcgag atcatataca ccagatggaa aattctctgc gtcgggaaag
     8161 gataggaatt tttctattaa cataagaaat actctagcct aaataaaaat atttgaaagt
     8221 gaaatttcct tcctcttctt acttttccta atttcttttt tagtaaattt aaattgaatt
     8281 atattctttc tccagaaact caacacctgt tcgaaacgga tcattttgtt tttttagaaa
     8341 gacaaaattt actttgtatt gttctggaaa ttttttaaaa tcctctgcca ttcgtttcaa
     8401 accagcagga caaagatacg caggagtgtt ctgtcccttc actcccaaag aaggaatctt
     8461 ttcatcaaag agtttttctt ctacatctat accaaataac gtttttaccg cttgattcga
     8521 aagttgtatg aaataaggaa tacgaacaac ggtatatcct aactttgtat aagtctcagt
     8581 aagtttaaaa tctttttcga taatatctgg cttcgtgtaa tgttgcaacc catcaaactc
     8641 aacaatcatt ttcaaacttt cacttctata atctggtcta gttctataca atttaccatc
     8701 caccaaaccg agagctttat catgaatcca atcattcgta tcagtaaaaa taattttcaa
     8761 atactcttct aatcctgttc tatgtaaacc agtatcctta tcaatgcctg cctttttcgc
     8821 aagtgcattc gtttctctta aaaatccgta atggggcatg ggctattcaa ttttattgtg
     8881 aacgtataat tctgattacg gattccctct aggtctatga ccatactttt tttcaaattt
     8941 agcaatataa tcattttcaa tttcttttgc tttcaccctg ccgggaatgc cagttattag
     9001 aatgtttgca atatacttag cataccctac taagcgatta aatatagtta attgttcatt
     9061 tgctcttttg gaagtgccat cagagtttaa aggttttcca caaattccat atttgaatac
     9121 aatattgtcc tctgaatcta cgatttcata cagatgatga tcatccatat tttcattaga
     9181 gtttttatga actgccattt cacttatcgc caaataccat tttcttcaaa tcaaaatcat
     9241 caccccaacc tttattatta gaaaatgttt cgggtatgta tttcggcaca tagttcattt
     9301 ctttacttgt tttaaattta taagcctctg ccggcaaata taaaatagcc tcaaaatcag
     9361 aattctttgg cattaacttt ggattcaata aaaccctagt ataaaaatct ttaccctttg
     9421 caacaactaa cgctctagta aataaaaata tatcaggaga aaaagaagaa tctcctttat
     9481 aagcataatc tccaatatgc tttgcatgtt cttttgtatc taggagaaac aatttctcag
     9541 aaagtatttc tttaaactga caaatatttt caacagataa tgaaactaat ctgtctagta
     9601 gaggtgaaag tattttagaa tccgtatctt gattccaatc tattaggtcg attaagtccc
     9661 aaaaataatc ctcattgaat agaacatctg tgtcttcttc caaaaaggtt acaattacat
     9721 ttgtgcgact ttgaatttta ggaagttgat ttaattcaat ttttccgttt tcatatttgc
     9781 cctttactga tatcatattc ccctctttcc tttaatctaa atattggaaa agattaagac
     9841 aacatttttt tgatgttatt ttaaaaaact tagaaagctc tatctcgcta gtcggaaact
     9901 gaacagaatt cgaatttcaa aagtcgacaa ggttgtaata tagaattatt ttcttgtatt
     9961 ctgccttccg aaagcaggtt ctctccttat aaaaatcatt cctcaataaa ataatcagta
    10021 tctatttttt ttatttcaaa acttcttttt gaagctactc ccacattata atcgatcatt
    10081 aagtttgcca attcttctcc attaattaaa acaactttga tatctatttt tgaaacgtag
    10141 tcgtatgaat tttcattgaa agatgaagta gttataaaga ttcctttttt cgctcgctga
    10201 ccttgcaaag cacccacaaa tttttgaatt tcaggtctac ctatattgcc ctcccatttt
    10261 ttcgcttgga tataaataat atccagacct aatttatctt cttttataac tccatcgatt
    10321 ccttcatctc caacttttcc agttatgctt cctctttctt ctaacgatcc accgtatccc
    10381 atttttatta ataactctac tacaagggat tcaaaaaact ttggagagca ttctttaatt
    10441 ttatttaaaa gttctattga taattcattc tttatttttt tataacctat ttccagcatc
    10501 tcatctggag ttaatgagtt aacttcatca ttttcattta cgctattatt ctcttctttt
    10561 tctttattct gccaatcttc tctttttctt ttatatggct caaaggttgc gagaaatttt
    10621 atattaattt tttcaggcgg attctttaaa acatttaaac cttcattcgt tattttataa
    10681 aaaccacgtc taggagattc aataattcca gccatcttca tatgcgcctc agcccaagct
    10741 actcggttag aaatcaatga aatggaacta gaggaaggaa gctttgcttc taaatctttt
    10801 tgcgttagtt taaactcctt acagatatga tcctttatat ctttactaga ctgctccttt
    10861 tgatctttca aatattttag taatggtaac attatacttt gaaaatctgg aatcgccaat
    10921 gtgtatctcc tatcttttat aattcaaatt atgaatcaaa tcttccctta cttctctgct
    10981 ttctaactat aatgtcaata gtaataattt aagtaataaa aaccaacaaa aatatagttt
    11041 taaattaatc taccacactc tctcccatac taaaaacctc atttcattat accaatatca
    11101 aaactatacg aaccactata ccaagtcagc agtaaatttg tagataatat gtaacgttca
    11161 tttccatatt ccaattccat tttgcctata ccaaattgtt gaaagagtta cgctgaaacg
    11221 ataaacaaaa ccaaagaaaa agcaaactca caaaatcatt cctcggattc aatcttgaaa
    11281 acccttcgtg tatcttcgtg cgcttcgtgg tagagaaacc ataacgacag attcaattct
    11341 cataaaaatg atttgaatta tcagtaattc atttttccat ttttctaatg ataaatttaa
    11401 ttcacgataa ttgattttct attccgttaa cttcaattca aggaatcatc ataataaatt
    11461 gcattgtagg aaacgtcagg tcatgtgacc tgacctacaa atcgcgaaat gatttcttcg
    11521 ttattggact ttcaaccaac acaagaccgc acatcgcgtg acctgttcta cgaaatttat
    11581 aaaggcaaac aatccaaacc catcagtgtg catctgtgtt tatccgtggt aatctttttt
    11641 cttttttaca tcaaattgga aaacagaata aaaatggatt actagtgaaa aaagaaaaag
    11701 atactataga ggaaatttat gagaatatgg gaatgagggc agagtttcat ccttacaaca
    11761 caattgaaca ggaaaacgat cgtgaaaagt agtcaaatta atttttatct aatgccagag
    11821 gactggaaag aagtagagcg ttatgttcaa aggaacggac taatgctatt agtctcccca
    11881 agccccgctg agaaactgcg agttgtatct tctataaatt cttcttccaa ttctgaattc
    11941 acaattcata aatattatac cgcgttaaag acagatgaag ataaaataat tcaaaagttt
    12001 gtcaaagcac aaaattatta tacaattgat gaatccaatt ctccggttgt ggaattacca
    12061 gttccgttct atcaaaacca tttagaaaag ttaaatcgag gtagattgta ttacacaaaa
    12121 gaattttatt tggagaaaac ctttgcgcca aaagagaatc aatttttaaa tatggcaaat
    12181 gatctcttta aatggtttcg taaaacattt aggactgata aattagatgg ttatgaagat
    12241 tttattattt ccccctctgc atttcaattt catcagaacg gtggaatttt attactgaat
    12301 cctgaattat caacatctat tccaaataaa gaaaaagttt tagcataaca tttcacttaa
    12361 ttctttatgg attagatttt acactaaatc ctatttatta taaaatgttt tttaatttct
    12421 catgtaagtc aggaatatca gatttaataa tttcccagac ttcttctgga acaacaccca
    12481 tatagtcatg agcaataata tttctaaaag attttatttt tttccatgga atcgaatttg
    12541 ttaactctaa agtcttttca tctaactttc ctactgtttc accgataact acaaaattca
    12601 taagaactgc atcgaaagtt ttttggtctg ataaaactct gtataatttg tatgttcttg
    12661 tataaatgcg agaatcttat ctatagattc aagaattaaa aataaattac ctcggtcttt
    12721 atcagataaa tatagcatct tttagaatcg attccttaaa aaaaggttta atatactttt
    12781 cacgacagag atcaactttt acaccgaaat gttggctaac gatttctcgg attctttctt
    12841 tggatgcaaa caattgattt gcactttcat cgaattcgaa tattaaatcg gtgcccatcg
    12901 gtggtattcc ttaaatgttt ttctaaattc aaaccgattc ctttagtcta aatgataaaa
    12961 atgaggaact atgaaactat ttattctttt aacaatattc agcttacaac gattattcgc
    13021 agtagaggtt gtgaaattca acggggaagc ctttgattta aaaacgggaa aacgactcta
    13081 ctacgatcac catgaagagt attatgaagg cggaaaacat ttatactcaa tcattcaata
    13141 caaagataca aatggaaaag tctttgcaaa gaagagaatt gtatttcaaa agagcccgac
    13201 gaaagcagat tttaaattag aagactttag agatggatac atcgaagggg cagaagtaaa
    13261 tggagatcaa atcaaatttc tctatcgaaa aaatgagaag gaagaattaa aagaagcaat
    13321 gattactgcg ccgaataacg ctgtgttaga tggtggtttt gattatttcg ttcgagaaaa
    13381 ctgggatatg attcttgcag gaaatcgaaa acaattgaat ctttgtgctc cctctcaact
    13441 cgattgtttt aagtttgttg tctataaaac aggagaagga aaaagaaaag aggcagatgt
    13501 tgtcatgatg aaagtagaat taaacaatgc gctcctagca gtctttgtaa aaccaatttt
    13561 attagtttat aacaaggaaa caaaaaagct tttacaatat gatgggattt ccaatattaa
    13621 caatgaagac ggcaaaagcc atgttgtccg aattatatat gacaataaat agaggttatt
    13681 atgaatttta aaagagaagc actcaatcta aatggtggca aagcagaaat cattacgatc
    13741 caaacaaatg aacaaaattc tttaacaaga cccaccatga aagaacttgc aaatatttta
    13801 aaggaaatac aagcagacga ttcctataaa ggagcaattc taacatctga aaatccaaaa
    13861 tttttctcca atggtttaga tgcagaaacc ttaatcaaca ctcctgctga tgaacttgtt
    13921 gaggcagttg gtgggatttg tattctattt ggagagatgc ttagatttga caaaccgtta
    13981 gttacagaag taactggtca tgcaatgggt ggtggagctg ttatgactat ggcgagtgat
    14041 tataaatata tgctctctac tgggtgtcgt atttctttta cagaaattgg atttgggttg
    14101 ccacttcctg gaatgtttgt ttataaatta caacaatcca ttcaccttac aaaattaaat
    14161 gaaatttgta tggaagctgc tgcctacaaa ggacccgaag caaaagaagt cggcatgatc
    14221 gatgaaattg caggaaccag agaagaaatt agaaaactct ccacaaaaaa attggaatct
    14281 gtattccgtt tccctttatc agctgtgcga tatacgaaac agaacatcaa cagacaagca
    14341 ctgcaggatt ttgatattca tctgaaaaat tctagtgatt ggttatatgt tcctgttata
    14401 cgaaacaata tgctagaagc aatgaaagca ctcaaagaaa aaagaagacc tgtttttcaa
    14461 taagtaaaat tttcttgggt gacaatccct actctcataa atgaaattac ttatacgaaa
    14521 cctttttgta taggtagttt cgttacctat acatctcacc tatagactca ttcccaaaat
    14581 tttcctaaac tcaaagcagt ttgaggaaaa aaaatgaata caaaatattt ttatcagctt
    14641 ggattgatac ttatcctaat tctttcagcc tgtgctacca ttgaaaataa tccctatgag
    14701 agtagattag cggatgccgt ttacgaacat aatttatatt ccgttaaata tgcattagcc
    14761 actggagcag gtatcaacga gaaaaaaagt ttctatggga atcatacagc tctcatgatt
    14821 gcctgcaaag aaggaaacta tgaaatagcc gaatggttat tagaaaatgg agcagatcca
    14881 aatatgacaa caagagatgg tcatactgct ctaatgcttg cggcttacaa ccgttatcca
    14941 gatatagtca agctactatt aaagcataaa gcaaatgtaa acatagtttc caaaagagga
    15001 catactgcac tttcagaaac aactaggact gattcagaga aaattaaagg ttttctaaaa
    15061 gaggcagggg cacatgaata gtttaaagaa aatattctta ggacttgtag ttctattttt
    15121 atttcatcaa tgcacaaata cgaatgcact tgcagcttgc agaaaaaagt gcacagaaaa
    15181 cgaatctctc tgtattgcag cgttatacga caaaggaaac tttaacaata atatttccgc
    15241 atgcgcgctt caacaggact cctgtcattt tacttgtgaa cgagatagta aattaataaa
    15301 gatatcacgg ggtcatggaa gcagtagtgg atttcgtagc ggtgggagcg gacatggaag
    15361 tagcggtcat agcagtggag gtggtggaca cagtagtggt agcggcggtg gaagtggagg
    15421 tcatagtagt ggtggcggtc atggaggcgg tggcggaatc atttttaatg atgctttcta
    15481 gaaattggac ttgtaaaaaa aatctttcca tatcgaaagt attattctaa aggatagaat
    15541 atgaattaca ttcgatatgg ttttattgca gcaggtttta caaacatagc tggtgttttg
    15601 cttttttcaa agttctttac aaatccattt atgaacgaag caagcccagt cgtaatgtca
    15661 aactttggtc tactcatgat cattgtttgg ggttttgcct atatcagtgt ttccactgcc
    15721 tatcaaaatg ttccctggtt ggttctttta ttcacagtag aaaaattaat ctatacaatt
    15781 tcatggattc tttggttcaa acaaaatgac ttaacctttc cctacacaaa agatcttttc
    15841 gcaggattgt tctatactct ctatgggttt aatgattttt tatcgatgtt attttttggg
    15901 tgggtattct ggattagccg caaagaataa atttttacag tccaggttct tccttgtaag
    15961 taaggacgac tgagaaacaa atctatgata gactctttct catggaaaat cttggactga
    16021 agaaaattct tctatcacta cattgtttac ttctatcttt ctgtttgaat gcagaacgat
    16081 ctccttttga tatgaataac caagcaagcc ctattggatc gcttctgatg ttttcaggat
    16141 ctggaaggac ttcagcgcta ccaagtccaa cgaatacagc attccaatat tcacaaacag
    16201 actacaccac ttacactgga tcgactttat cgatttcagc cccctctatc accagtggat
    16261 ctgtatcttc tttctctatc tctcctactc ttccaaatgg cttagccctt gatacagcaa
    16321 ccggctctat caatggagtt ccgacaggat ctacctccaa ccaaacctac acaattacag
    16381 gcaaaggaca aatcaatgat gcaacttcaa cactgagtat taaaattgga tccacatcag
    16441 cggctggtct agttggacaa tctagtttta attttagtgg aggaggatca ggcaattctt
    16501 ccatcaatgg tttgtatgat atcagcttgg atagtaatag taacttgtat attgctgatt
    16561 caaacaataa tagagcatta atttttttat cgggaagttc ttcagcctcc actgtttttg
    16621 gacaatttgg aagctatgct tccatagcaa tcaataatgg aggcatctct gcggattctc
    16681 ttttttctcc gcgtggcata accgttgata attctggtaa tttatttata gcagatacaa
    16741 gcaataatcg aatcttatac tatctccccg gttccaccac tgcaacacgc gtctatgggc
    16801 agctgggaaa tttcactacc ggcacaatca acaatggagg aatttccgcg aactctctcg
    16861 ctggtcctta caaacccact gtagatgcag atgggaattt atacattgca aatttatttg
    16921 cacacagagt tttatattat cctgcaggtt ccacaacagc tactcgtgtc tatggacaac
    16981 tgggaagttt tacaaccaat actctcaata atggaggcat ctctgctgat agcttaaatg
    17041 cacccgtagg aacagcgtta gacgccaatg gaaatctata cattgcagat agacaaaaca
    17101 atcgagtctt agtttatcca cccggatcta caactgcaac taaagtcttt ggacaatttg
    17161 ggagttttac aacgaataca gcgaataacg gtggagtatc tgctaatagc ttaagtgctc
    17221 ctactggtat aactattgac acaaatggaa atgtttatat agcagatagt gcgaatcaca
    17281 gagtgttata ctatccgact ggaacaacaa ctgcaactag ggtctttggt caaatgaata
    17341 gctttacaag taatactgct aataacggag ggataacagc tagtagcctc aacactccct
    17401 atagcgttgc agtagatatt aaaaacaata aactctatgt agcagacaat ggaaataatc
    17461 gtgtcgtaat tttttatgtt ccctaaagaa aattaggagt ataaatccgt tttggagaga
    17521 atctaagact tgctaattca ggcgctcaga gaaaactgtt cttagctcta ggctaattta
    17581 attttcatag gatgtaaaca tggaacaagc agtaatcatt gatggcgtaa gaagtggatt
    17641 tggatctttt ggtggatctt taaaagactt tggcgcaaca gaaatgggag tagaagtagc
    17701 aaaagctgcg attgcaaagt cgggcgtaaa agcagaagat attggagaaa gtatttttgg
    17761 aaacgtagtt ccctctggaa aggattccat ctatctagca aggcacattg gactcaaagc
    17821 aggcctaaaa gtcgaaaccc cagctctcac agttaataga ctttgtggct caggaatgga
    17881 agctatcgtt cttgcagcaa agaagattta tctaaatgag actaacgtag tattagccgg
    17941 tggttcagag tcaatgagcc aagctcctta tgtagtcaga aatgctagat ggggtataaa
    18001 atatggacca acagaattcg aagatagttt aaaccaaggt ctcaccgatc tatatgtaga
    18061 attaccaatg ggtatgacag cagaaaattt agcagttcaa tatagcattt ctagacaaga
    18121 acaagatgag tgggcagccc tttcgcaaga aagagcagaa aaagccacac tagatggaat
    18181 attgaaagag gagataacac cgattaccct cccaggaaaa actccaatcg tttttgataa
    18241 agacgaattt ataagaggaa aagcatctag agataaaatg gcaggtttga aaccggcatt
    18301 caaaaaagat ggaacagtta cagcgggtaa cgcatctgga atcaatgatg gagctggtgc
    18361 tgtgattgta tcttctaaat catatgcaga taaattagga aaaaagccac tagctattat
    18421 aaaatcgtat gggcatagcg gttgtgatcc agccaaaatg ggaatcggtc ctgcacttgc
    18481 aattccacgc gctttatcct ctgctggtct ttctttaaaa gacattggac tcatagaggt
    18541 taacgaagca tttgccgctc aataccttgc agttcaaaaa gagttaggat taaatccaga
    18601 gattacaaat gtgaatggtg gagcgattgc aatcggtcat ccactgggag caagcggaac
    18661 tagagtaaca ctcacacttg cttatgaaat gagaagaaga aaagttaaat acggagttgc
    18721 ttctctttgt attggtggtg gtcaagggat tgcgattgtt ttagaaaatc caaatctcta
    18781 atcactgatg gaaagactga gtgcaattaa gtattcagga aaaataaatt tttcagaaaa
    18841 ttccataccg atcataacgt gtggtttttt ctgaaattcc atttctgttt tccatgcaac
    18901 tcttccagag atatccatat tatcacttgt aggccaatgc aaaacacttc cttcaagcat
    18961 tgcattcact tctacattga aactagccgg catgatagca caaagacctg tttcggaaat
    19021 attgcctaga tagccgctaa aactctcccc agcggcgttt agatgaactg cataatctgc
    19081 aaaatctttg ggaaaaattc tatctgactt tctaatctct gttttcataa cccttgtgta
    19141 aaatacatat tgtaaaatgc aaggactttt taaaataacg aatagcacac tgcgtgcaat
    19201 gctctagaat taagaaaaac tcataaagac taccaccgat tgccgccgaa caagcggcaa
    19261 tggattttgg gtggtaagac gatgagcacc gataaaaaaa tatgataatt ttaaattgat
    19321 caatcgtttc tgtatccttc tgttttcggt gtccttagtg gtaatcattg attttcacct
    19381 tgaacaagat gagcgaatta aagaatcaat tgatagaatt tttaggtttt gaatcttgga
    19441 atgaatctat gataaaaatt ggcaatgttg aaattccgaa taatatcgtg ctctctccaa
    19501 tggcgggagt ctcagatagc ccatacagac agatttgtag agagatggga tcaggctttg
    19561 cctatacaga atttgtttca accgatggaa ttagccacca ttccaaaaaa tcaattgatc
    19621 tattccgatt ccagaaaatg gaaagaccga tttcctttca gatttttgga aataatttag
    19681 agatcattac agaagcttgc agaatcatag aagacctagg accagacatc attgatttaa
    19741 atatgggctg ctctgttgca aaagtttctc atagaggatc aggcgctgga cttttaagaa
    19801 agccagaata tgtaggtaag atcattgagt ccatgacaaa agcggtgaaa gttcctatca
    19861 ctgcgaaaat tagaattggt tgggaccatg caagtttaaa ttacaaagag attgtgcatg
    19921 ttttacaaga gtcaggtgtg caagcaatct ctgtgcatgg aagaactaaa tcaatggcat
    19981 ataaaggtct tgctgattgg aacgttattg cagagatcaa atcttttgcc aaagttccta
    20041 tttttggaaa tggcgatatt caatcctatg cacaggcaaa agaaagaatc aaaacatctg
    20101 gagtagacgg agtattgatt ggtagagcag ctatcggaaa tccttggatc tttgctggaa
    20161 ttgacaaatc aacagtatcc tttccagaag ttcgcgcgat gatctttcgt catttgagtc
    20221 tcatgacaga tttttatgga gaacaaatgg gacttgttct tttcagaaaa catgtttcaa
    20281 aatatctaag aaactatttt ggaattgcag acctaaggca taagttagtt accacaaaca
    20341 gcttaaatga gtttcatgat ttattaaaca attttgaaat taaagaattt aaaatggttt
    20401 cggaagaaga aaacgaaaat ctcaactgtg aaacctatgc ttccgcagag taattctata
    20461 caaaataaca aaagtaattt tctataagga ttttgattga gtagcctttc gtatatcgca
    20521 catacagacc tgagagaact ctaagaagtt ctatacaaac ctacctttta tttttaaaat
    20581 ggaatttgct tttgatgttt gagataaata acgtgagttc ggcataagga attgacaaat
    20641 tgttcagacc gtcattccca ttatgtttcg acccaaggaa gaaacattga gttgcgttcc
    20701 accctacgca ggtgctggat ttgcgaccga gcaaataggc ggaatagccg caaccgccaa
    20761 gcgatatcga gcaatatcat taagttaagg atttttgtgt cattctttaa agctgtcact
    20821 cccgaaatcc cctatcggga gtttatgtaa tgataatttc gagagacccc tgataatcgc
    20881 ctccgcggca ctggatgtga gtgaatagta tcgggtggaa ttcgggggtg aaaaaatgaa
    20941 acatcttttc atgtcgaatt cacgttaaat aaaagaaaat taaatcagtc ttcaattttt
    21001 aaaatttatt taaaaagaat ttacatttag caaatagggt atagaatcta gaaatctatt
    21061 catgctatga tggtagtcca atgaaacaat tttatgtaat ttttattcta atatttattt
    21121 cttcgaacaa tctcctcgct caagcaaatt gtgaaaaatt tgaatcctta aaaaaagcat
    21181 ttatggataa taaaaaagaa tctgtttgcg aacttgattt acgaaatcaa cacttgaaat
    21241 cacttccaac gaatatgggc gatttaataa acttagaaat tctccattta gaaaataata
    21301 aattatccga attgccagaa tcattagttt atgctaagaa actagtcaga gttcacttag
    21361 acaacaatca attcagaaaa tttcctgaag ttttattgga tttagatcag attgtaagaa
    21421 tctcaatgaa ccaaaatgaa ctaacagaaa tacctacaaa gattgaaaat ttaaaagaat
    21481 taaactactt aagttttatg ggaaacagaa ttgagtccct acctaaacaa attgggaaat
    21541 taaaaaaatt aacctattta aatttaattt caaatgattt gaaagagctt ccccaaatgt
    21601 tttataattt acatcatcta gaaaaccttt atctcggaaa taatgatagc ctaaaaattt
    21661 ctgatgaaat ttacaaaatg aaaaatctaa ttcgcctatc gattagtgga atcggactgg
    21721 aaaatctttc gacagatatt aagaatttga aaaatctaaa atggctttcg attagttcaa
    21781 ataaaattga aactcttccg gatgaaatct gtgaattaaa agatctagag gcattctttt
    21841 tcggttataa taaaataaca aaactgcctc atagatttga agaactaaag aaattgaaga
    21901 aaatttcttt agtggatcta gatcttggaa taaccgttga aaatgattta agaaatacat
    21961 ttcctgaggc agaaattgaa ttttaaatta tgcgacaaat aatactttgc cgcttgtcat
    22021 attatttttg tagaactcaa ttgctttgta tccatcatcc aatgaaaagc gtttgttgat
    22081 ttctgactta aaatcagtgg atagattttc ttgcgcatct ctaacgattt cagcaaactc
    22141 ttcttttgga gtatctgcaa tccaataact caaccaaaaa ccctctactt tctttctttg
    22201 aaaaatcaaa gaacctgctg aaacaccaat cgggtcctcc gaaagtgcac catagacaac
    22261 aagcttagaa ttaccaggca tcatattcaa aacatcttcc ccagtctttc ctgcaactgc
    22321 atcaattgca taagtgattt ccagcttctt gactagtctt gtaaaattct tttcgtaatc
    22381 aggtgatgta gaatttaata catgctctga tcctaatgat tttaacaact caacttgatc
    22441 atcttttcga acaatattaa taacaggtaa tccttttcgt ttcgcatatc gctgaatcat
    22501 cttacctaat gcacttgctg ccgcagtctg cacaataccg cctcttttct ctttaatcgc
    22561 aatgttagtt aacccacaag ctgtaagagg attaacaaag aaagtagcac cttgctctaa
    22621 agtaactttt tcaataagcg gaatacagtt gtattcgggg gttaccatat attctgccca
    22681 ggatccgtct cccatatagt gagcactgca agcaactcgg tcacctactt tcactcgtgt
    22741 cacagcagat cctattgcta ctatcgttcc gctaccttca aacccaccac cacacggtat
    22801 ttttttctta attccataaa gtccgcggat aaacatcaaa tcagacggat taatagaaga
    22861 tgcataaatc tttacaagaa catcagtttc ttttaaacta ccaatttttc tttgggcaac
    22921 acgaagcgaa ctttctaagc catcataatt aatcagttca atcgctttac ctgtttctgg
    22981 aatactcgtc attgttttct ctcttgaatc tattttagct taaattaaaa atgattcttg
    23041 tttgcgtaaa gcataaatta tcaaagagag aaattataca tttacttgtc ggtatataaa
    23101 tataccgaca aaattttatt aatgatggtg atgatgctgg ctttcggttt cctctgcttt
    23161 atctacttct ttatcagatt cttgattcaa actggatcca cccatatgat ggtgatttgc
    23221 atgattatga ttcattctta aaaatacagt gcttaagccc actgtgataa ttccaatccc
    23281 aactaaagca gcataatctt ttagaacata ggattttatc ttatgaaatc ccagtccgaa
    23341 gagtaaaaga cctggcagcg ttcctaggaa aaaggaactc atagaaattc cacctaacat
    23401 cggattgcct gtggcaagag acattgcaaa tgctggatat aaaattgtgc aaggaagaag
    23461 acctgtaaac attccaaaaa taaatgagaa taataatgtg tgggttgact tcctcattgc
    23521 ttccataaat ggatgaaata attttttaat aatcttattg gcaccactgt ctggaattaa
    23581 tttcttattg aatattaaag caatcccagt tgctaaaata aaaattgtgg agagtataat
    23641 tgaaatattt tgaatttttg taacttcgct tgataagttt aaactaaaac ccaaaaagcc
    23701 cattaaagat ccaatcaatg tatacgatgt aaatctaccc aaattataaa tgacatttgt
    23761 tgttagtttg gattcaccta taatattgag cattcccaca aatggtccac acattgtgat
    23821 acaatgaaat gaactactaa agccataaat aaaagaagaa ataaaaataa tttcgtaatt
    23881 catctccaac tctccttctc gtcctcaaaa agaattctgt atttcggagc ttcaatatct
    23941 tcgaactgtc cattcttgat tgaaattaaa aaaactatta aaccaataaa tgcgataata
    24001 aatgcaatcg caattgtaat caataatgct tccatgttat accttcctcg caaataatct
    24061 taataaaaat gaattagcta ctacagtaag agagcttaat gtcataaata gcgcacaata
    24121 cacaggttgc atgaatccaa aagcggctaa cggtaacata atagaattgt acatgaaaga
    24181 tattccgata ttttgcttaa tggctctaga agttaacctt gcatactcta tagagagccg
    24241 aacccctttt aagtcattct tcatcaaaat gatatctgac ttatctatag aaatatcaga
    24301 agctaattcg agagaaattc ctacatttga tttagcaaga attccagcat cattaattcc
    24361 atcacctacc atacctacaa tttttttact agcctgcaaa tcattcaaag cttgtaattt
    24421 ttcttctggc ttcaattcac ctttataatt ttcaatacca agttccttcg ctattcgagt
    24481 tacattctta ttattatcgc ctgataataa ccgtatgtct ttcactcgat tctttaactt
    24541 taaaatttcc tcttttattc caactctagg tatatcgttt aaccgaatga gccctatgta
    24601 tttttcattc acagaaacat atacgatagt tcctagattg gtatcttcaa tttttatatt
    24661 ttccaagctt acatattttt gtataaaatc taaatttcca atgctaactc tatattcttt
    24721 attcccatac aataaaatcc ctcgaattcc atacccttgt atttcctcta cctctttcac
    24781 gtccacaaaa gcatcattga tagagcttgg atttgagtag gcattcacaa tcgactttgc
    24841 aattggatgt gttgattttt tttcgagcga gtatatcaat ttttgattta gatccggatc
    24901 taggattgtg tcctctatta cagaaagagt tccctctgtt aaagttccag tcttatcaaa
    24961 aatcagagta tctaatttag agaggggctc gattatatct aaattcttta aaataattcc
    25021 tttttctgaa ttgattatat tattaatcac aagagtagaa ggaatcgcaa gtcctagagc
    25081 acaaggacaa gcaacaatca ataccgcaat tgagtaaatc aaagatcttt caaaatcaga
    25141 ggaatagagc atccatccaa taaatgtaga aaccgcaaca aataaaacta cactaataaa
    25201 ctttgtggca attcgatcag tttttctctg tatagctggt ttctccaata acgcatcttc
    25261 aatcatcttt tgtaagatcg atagagttga gttttgagta gaattaatta ctcttaaata
    25321 aattgcattt gtaatcgaaa gcgaaccagc gtaaatcttt tcgccttttt tcttggatac
    25381 tggttttgat tcacctgtca gaaaagactc atctacatgt ccatggtcag attctaaaac
    25441 accatctaca gaaagtcttt ctcctgcttt tactaagatc aaatcatcct tcttaactcg
    25501 attggatgga atcttttgtt caactccatt ttctatgatt gtgcaaagct cgggtaactt
    25561 actaagcaaa ctatttatct ttcgatttgc catatttcga gagacagctt ctaaatattt
    25621 cccaattaat ataaaaaaga aaatagtgca aacagaatca aaatacactt cgcctttgtc
    25681 ggttaacgtt acataaacac tataaaaata cgcgagcgaa atacctaaaa ttaaaagaaa
    25741 atccatattg agagttttat tttttaatcc atagtaagca ccgcgaaaaa aaggaacgcc
    25801 cgaatagaga tacacgggcg tggcaaggag ccaagagtag aaatggaata atcgtttaaa
    25861 accaagttca atccctgtaa aatatcctgc atacagagaa acagtaaatg ccattatgct
    25921 tccaaatgaa aaagcagcta ctaccatccg aatcagtaga tctgttgcat aattgttata
    25981 ggctttttct tttttccatg gtgcatatag ctttggtaaa tacccaatac tttgaataat
    26041 agaaaaaatt tgagacaatt tgagaatttt atcatcccat acaatatgcg ctctacctgt
    26101 tgaaaaatta attcttactt tcttaattcc ttttgtctca gataaaactt tttcattgag
    26161 ccatacacaa gcagagcaat gaatattggt aatattaata tatacttctg aatcagtttt
    26221 attaacggaa gtcacaaatt cagaataggt tacttcactg tctaagtttt cagcttcttc
    26281 ttttctatat tctgcaactg gctctaagtt ggacgatcct cttaaatcat aaaagaaatc
    26341 tttacccatt tcggaaataa gttcacttac agtttgacag ccggtacaac agtaatatct
    26401 atcctctcct tgaagcttgg atgtaacaat tagatttttg ggaatagggt tttggcaatg
    26461 gtagcatgta gtaggaatag aactcatttt ctgcgatttc gtatttttat ttagagactc
    26521 aaactctttg gagagaacca tctttatttt accacgatca taaaagtttt ttcaaaactt
    26581 ctaccatcta cacttgcatt taaagtgact tgccatggtc catcaagcgg aaaatcaaca
    26641 ttagccttat acattccatc tttatcataa ataaattctt tcttttcagt aaatttattt
    26701 gtagcacttc tctctcttgt taaaactagc tttgcacctt tcactggttc ttcattttta
    26761 aagataccaa tagaaagttg attatttccg agagtaactt cagaatcttt ttctaagagc
    26821 gttgtgcgaa aatgatatcc ttccgaacgc atttgaacgg tttgagcaat ttctttctca
    26881 tagttcatac ctttttcgta atagttttta tccatgacag gttcatgacc tgccatagca
    26941 attttcactg tataccaagt ggcagcaatt aaagcagtaa acgcaagagt cattactatg
    27001 aatgcaaagc gtatgcttcc actttccgat ttaatattat tggatacttg gatcatctgg
    27061 taacctcaac ggaattgtct tattaatttt ataagaagtg ttgcttgtat cttgtaattg
    27121 caatttcacc tgcacgatac ttgcagcttt ttctgatttt ggtaaatttc cggattcaat
    27181 aaagattcta agctttgcag ttccatttgc aggaactagc acttttgaat cttctgttcc
    27241 agtgtggata accggcttta gccgttcatt gtccacaact tctaacttta attccctatt
    27301 ctcataagtc atattttgaa gatttaagat ataaaaattt cgaacttttt gatccgggat
    27361 aatcattggt tctaaacttc tttctggaat gatactagcg tagagcggaa ctctatcgga
    27421 cagtaatacg attgctgttg ttaaaaccgc aaataataat gtagcataaa tgacagttct
    27481 tggacgaatc caacgaatct tttgattttt ttcaactcga ttgagcgagt cataattaat
    27541 taatgatttt ttattttctt tgcccatttg aattgtgcag gcatctatac attttccgca
    27601 tgcgatacaa cccacattta atccgtctcg aatatcaata cctgttgggc agacgactag
    27661 acacatatta caagctgtgc agtctccaat tttaacttgc ttatttctgc gaggctctcc
    27721 tcttttataa tcataggtta cgttatacga atcagcatcc atcatgactg tttgaaatct
    27781 tgcatacgga caagcatact tgcagaattg ctctctaact actgtcatat caataaagag
    27841 agttgctgag aaaaatgcta taaagtattg aaacttcaca tctttaaaat ctaaactcat
    27901 aattctagat agcatttctt ttgggtcatt gaaataagcc acaatatgga atgcagagaa
    27961 aattgagaaa gctatccaaa cagcgtataa cagcgctttg ccgtaaggat tagcatcttt
    28021 ctttccatat ttattgccta atatgaatct accgataaca tcaaaaaaat cggtaaacac
    28081 tgtctgcggg caagcccatc cgcaccacac tctaccaatg agagatgtga aaaagaaaag
    28141 cgatagtccc gcagtcagta gaaacaagtg taagaaaatt ccttcttggg gaatgtaaat
    28201 tccacctaat agatgaaatt tccttgctgg aatatctaga cgcatgaaag gtttgtcccc
    28261 ccaagacaac caagggatga tcacataaat caaacccagt aatgcctcaa cgacatagcg
    28321 cctactcctg attgcccccg ttatatgtct agatataatc atttctttac tcctatttca
    28381 ctttttttaa acttggattg ttacttgcaa gccaagccat tacttgatat actctctcag
    28441 agcctaaaga agctttatgc ggaggcattg cgcccctacc aagctttgct ttatcaactc
    28501 caaccccttc cataatgaca ttgtagattt ctgcatctgt gtcaccatgg atccattctg
    28561 catcagtcag gtttggtcct actaatcctt ttgcctctgg tccatgacaa gcaacacaaa
    28621 agttctggaa gtttttcttt ccttctgcaa tcgcatctgc attttcacgc attggatttg
    28681 atccgtctgc actaactaca acctttgcca tcggaaattg tttttcatat tcagcgactt
    28741 ctttattaaa actatcagcc attttccatt cggaaaacca gtgaaagtaa attgtataaa
    28801 acactgcgaa aacaattgca agtccgaaga aaagtttcca ccattctggc atcggattat
    28861 ctgcttgttt gataccatca aattctttat tatgatccat atttagtcct catctaacat
    28921 tctatatttt ggagtttcaa ctaattccct tcgtttttta ctatataagt aaaatgtaat
    28981 aaacataatt gctattacta acacagggag cctgagcgct ttgtaaacta ccagagcagt
    29041 catcatttga tcttccatgg catttcctac ttagcagtct cctgcaaatt agcagtatca
    29101 cgtcctaatt tcaaaaggta tgcaattaac gcatctccct tagttttacc tgctaactgt
    29161 gactctacta aatcataatc agcttgcgaa taaggaactc caaccttaga aagaccttgc
    29221 atatggcttt ttatatttgc agggttaatt gtagcacccg catcaaataa ccatgcatac
    29281 gatggcattg ttgaaccagg tgaagtttct cttggattca tcaagtgacg tttgtgccaa
    29341 tcaactgaat cctgaatttg tgattcgtga gctaaatcag gacctgttct tttagatccc
    29401 cataaaaatg ggtgatcata tacatattcc ccacctttag agtatccatc tttaccataa
    29461 actttctttg gatcaaaacg atccacttcc catttaaaag ggcgaatcat ttgggtatga
    29521 caactattgc atccctctgc aatgtaaata tctcttcctg ccaattcaat tggagaatag
    29581 ggttttactg aagcaatagg aactaccgtt tgtgtcaaaa agaatggagg aatcaattca
    29641 aataatcccc caatcaagat tgcgatagtc gtatagattg caaacttaac gccatttaaa
    29701 tcccaatgat ctgcgatatc agaaaaccaa tcaaagattt tttgcatcat ataagcctcc
    29761 ttatgccttc acccgaaggt ctatttcctt gaaaccgctt ccagctgttc caattgtttt
    29821 aaccaagtta tatatcatga taacatagcc gatgaagaac attcctcctc caacccctct
    29881 catgattctg tatggtttta gaagtgcagt aatctcaacc cagtttggat attgcaagaa
    29941 acctttatca gtaatagctc tccacattgc accttctgta ataccagaaa cccacataga
    30001 aacgatgtat aataaaatac ctaacgttcc taaccagaaa tgtaaatttg ctaatttttc
    30061 tgaatataga tttgtattcc acattcttgg aactaaataa tagaatgcag cagcagacat
    30121 gaatcctacc caacctaatg ttccactatg cacgtgacca atgatccaat cagtattgtg
    30181 agctaacgca ctaattgcac gaatagaaag aagtggtcct tcaaatgttg acataccgta
    30241 gaaagtaatc cctacgagca tcatcttcaa agttgcatct gttttaattt tatcttttgc
    30301 ctgtgtgagt gttaaaaatc cattcaacat accaccccag gatggcatcc agagcatcac
    30361 actaaaaacc attcctgtag tttgtaacca ttcaggaatt ggggaataga gcaagtggtg
    30421 aggaccagcc caaatgtata gaaatactaa actccagaag tgaatgatag atagtttgtg
    30481 actataaatt ggctgtttga tgtgttttgg gaaataataa tacatcaaac cgaggaacgg
    30541 agtcgttaat acgaaagcaa ctgcgttatg tccataccac cactgaatat ttgcatcata
    30601 cacacctgag taaatcgaat aagatttcat taatcctacc ggaattgcta aattgtttac
    30661 aatgaataag attggaacgg tcacaaaaga tgcaacataa aaccaaatcg caacatagag
    30721 ttgtttttct tctcttttga taatagtcat gagatagttt acaaagaaaa tcacatacca
    30781 aataacaatt aataaatcaa gtggccactc taactcagca tattctttac cttgggttaa
    30841 acctaatggc aatgtaattg cagcaagcaa aattgttaca ttgtaaagca ccaagtgaat
    30901 cattgcaagt tgatcactcc aaattctaac tcttagtaat ctttgtgctg aatgatacgc
    30961 agtcgcaaaa atcgcactga gcgcaaaacc gaaaatagcc gcatttgtgt gaagcgggcg
    31021 taaacgacca aagctaaaat actctgtata atttaattgt ggataaacta attgaaatgc
    31081 aatcaatact cctattgcca tagctgcaaa tccccaaaga aaggctgaca gaataaacgc
    31141 ttttacaatg aagtcattgt atttaggatc agaacctgca gatgcagagc ctgaaccagc
    31201 ggatgtattt gtgttcataa ataattcctt ctccttaaag taccgtatag aataaatatt
    31261 gtaaaagaat caaagggttt ttataagttt tgctttcaaa ctaatatgat ttttgtcata
    31321 tggtaaaatt agagaaaaaa aatttagaat tctataaata tataaaaagt ttcacgagga
    31381 aggaattagt gtattaccgc ttcttcgtaa ggattaaaat ggaaaatagg atcaattgtt
    31441 ctagttgttc aagcaaagaa tatggtgttt ttaaatgtgc ttctccaact gcgttagaca
    31501 aaatctatgg agataaatct catcttcaat tcaagaagaa agatatcatc tttaaagagc
    31561 atgaaaaaat gaatggcttt tactgcattc aaactggcgt tgttagaact tacaaaacat
    31621 ctgctagcgg caaagagcaa acatttcaaa ttgcaggacg tggcaaatgg ttaggatttc
    31681 gagacctaat ttcaggtgaa gaatcaaatc attcctcgat ttgcttagaa gatgtagagg
    31741 tttgtttcat accgaaagaa acaattactc atttaataaa gtcagatgct gactttcaaa
    31801 ttgaagtaat gaaatatcta gcagatgaat ggaaagatat ggagaaccat gttcattcaa
    31861 tgggcacaaa gcagattcat agcagattgg cagaattgct catcacattc catggagcgt
    31921 caggcggcac agaaaatgag ttaaacatta atgttactcg agaagtaatg gccacctgca
    31981 ttggaactac gactgagact ttaattcgtg ctttagctga ttttaaagat agaaattgga
    32041 ttcgagtgga aaagaataaa atagagctaa tcaatttaaa agccttatac aacatttcgg
    32101 aagcaacaac caattaactt cggttatttg cgagtttgtt accagccact agaaaataat
    32161 ctccgtgccc cttttaagcc acatttttga taaatttggt aggctctggc ggcatcatcc
    32221 ctgatttgac tggcattttt attttcaagc cagcttaaaa ctccggcatt taaaacatat
    32281 gcttcaggag caaggagtcc atttgtccag agaatagggc ttatttcact cgagttaaat
    32341 tgactcgaaa aataggattt agaataacaa gcaagtagaa taatcggtat agatttgtag
    32401 ttatttattt tttttaaagg caattctaaa ctaaaatcca ttaaaccatt gtgacctaca
    32461 aaggcgagta aatcggcatt agccgctaat tgtaatagaa tttctttttt taagaatggg
    32521 ttatagatca gtactgaatc tagatgaatt tgcttcgcag atagaattaa attttctata
    32581 gattttttta tcgcacttcc tttgtatgca tccgcaatta gataagcatt tttcgtagga
    32641 ttccaaaata caattctctc caatataaaa tcatttaaat tcagtgaact agaaatgatt
    32701 ttccaatcct tttgtgtttt aaaataggat ttcactccat aacgcgcgcc ccaatataaa
    32761 ttattttcta aatcttcacc atttcctaat ttttctggaa caggaacaat tccttggttt
    32821 ttattatcac agagcgaaac aaaaacatga atcacctttt cttcttttga taaaattcct
    32881 ccagagaaaa gatttaaaaa gaaaataaat ataataattc taagtatcat gagtaaatga
    32941 gtttattcac tatttatttt tgtatatatt tttttaaaga atgtttctat ttgtgtataa
    33001 cgtctaaaaa tcataatgat tcatatagaa aaaaaatact ctttggtata gaaagaaagg
    33061 aaagaaaatt gaaaaatatc atttcttcta gtcgtttcaa tctttcctac actaaataaa
    33121 ataataatat atgagcctag ttgactctct gaatcccctc atttacccaa agggaaaaaa
    33181 ataaaaacta tgaatcgaat ttcgcaaagc aagctaagca ggcttattag gttgactaac
    33241 tgagtagaac ttttcaccaa aatgttttta taaaaacgta gataagattt tccaaacctt
    33301 cctttctcca taaatttcta tcataccaat tagaatcaga aaagaagata aagattgtat
    33361 atcctatgca aaaaaagatt ctctctttag gacattctta caatttccca atcagaaagt
    33421 atccaaccga attgaaaaag gaattgaata tatgattcaa gaaaaagtaa gttagttgaa
    33481 acataattga tttatggaat aaaaatcaat tcagcgcact gacttatatc atatatcgtt
    33541 cctaaaaatt atgagtgaga aactaccgta ttcttttcca acatggttaa aatacagtga
    33601 ggactatatg aacaagtggg tatattcttt tgatgaatta aatcaagcgg aagagaaaat
    33661 gggaggagat tgggataaag tgcgaggtct tttaggaggc aaaggtgcaa atcttgccga
    33721 tatgactcgg gctcagattc cagttccacc aggatttaca gtaacgaccg aagcgtgcaa
    33781 tgcttatata gcaaatgacg gtcactttgt ggaaggtata tgggaacaag agatggatgc
    33841 tcttcgtgct ctagaagcaa ggactggtaa aaagtttgga gatccttcca atccactcct
    33901 agtctcttgt agatcaggag caaaattttc catgccaggg atgatggata ccgttctaga
    33961 cattggatta aatgatacga ctgtagaagg aatggctaca ctttctggaa atgtatcctt
    34021 tgcgtatgac tcctatcgaa gattgattca aatgtttggg tccgtcgtaa ttggaattcc
    34081 ggatgacgcc ttcgaggaag tattaaaaga agcaagagaa gaagcggaag tagaatcgga
    34141 ttctgaccta gatgcagaat ctttaaaaaa aatcattcag aagtataaat ccgttttcaa
    34201 acagaaagca tggagagaat ttccacaaga tccaattgaa caaatacgtc ttgctacaga
    34261 agcagtgttc aaaagctgga atggaaaacg cgcaattgat tatagaaatg cggaaaaaat
    34321 tccacacgat ctaggaacag cagtgaacat cgttgcaatg gtttttggaa atatgggtaa
    34381 cacatctgct actggagttg ttacaactcg aaatatttct acaggcgata gagaattaga
    34441 aggagactat ctcattaacg ctcaggggga agatgttgtg gcaggtatta gagccacaaa
    34501 accaatttca atgctgaaaa gagaaatgtc tgattcctat gcagaacttt taacaatctg
    34561 taaactacta gaaaaacatt ataaagatgt gcaggatata gagttcacaa tcgaaagagg
    34621 taaactttgg attcttcaaa ctagagacgc aaagagaact gcacaagctg ctgtccggat
    34681 tgcggtcgaa atggcggaag agtggattat aaataaagag caagcaatgc ttcgtgtaac
    34741 tcctgcacaa attgatttct ttctacatcc tcagtttaaa gcagaggaaa aagaaaaagc
    34801 agaacataca ggacaacttt tagctagagg acttaacgtt tctccaggcg ctgcaacagg
    34861 cgttattgca tttgatgcag acctagcagt taaatgggca gcagaaggga aaaatgtaat
    34921 catggtcaga ccagaaacaa aaccagatga tgtgcatgga atgcttgctg caaaaggaat
    34981 tctaacaagt agaggcggta gaacaagtca tgctgctttg gttgcaagac aatttggtaa
    35041 acctgctatc gtcggagtaa atgaattagt gcgactttgt caagtcgcgc acgtttgtaa
    35101 aatagaattg aagtggtca
//
LOCUS       DAOQOS010000052        34176 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_537_length_34176_cov_5.558366, whole genome shotgun sequence.
ACCESSION   DAOQOS010000052 DAOQOS010000000
VERSION     DAOQOS010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 34176)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34176)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34176
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_537_length_34176_cov_5.558366"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..669)
                     /locus_tag="PLF66_12840"
     CDS             complement(<1..669)
                     /locus_tag="PLF66_12840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64977.1"
                     /translation="MNENKNIKESKKKFHDSSKKKNYKPVNNKFTRDVNQEERRFKKN
                     TEEKNFFGFQKNKDSESNFSKNRKFSRDFEDNSFGSKRNTFKKNDRRPKSNQFKTNGE
                     SESFSENRFKSNTRPNSGKKFSFRDRNQSEGGGDKERGRYWSKGANNRNPMSGNRFQP
                     EREIQDDIGNRKPRFERQERDDIGNRKFENRKFQDKKFGDRKFGDKKFEDKKFGDRKF
                     GDKKF"
     gene            769..1686
                     /gene="lipA"
                     /locus_tag="PLF66_12845"
     CDS             769..1686
                     /gene="lipA"
                     /locus_tag="PLF66_12845"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010569602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HMZ64978.1"
                     /translation="MNPLKKKPRTKNTSPMGEKPEWLRVKVKFPTEQDPVSSVRKNLT
                     NTRLNTVCESASCPNLNNCWSRKTATYMLAGDICTRKCSYCDVASGKPLPLDKEEPIR
                     IANSVRELGLRYVVITAVNRDDLKDGGAMHFHQTVQEIRKQSPECRIELLIPDLKGKR
                     ESLDLIYSANPDIINHNIETVKSLFKEVAPAKNYETSLSVLKDISEHGFITKSGIILG
                     LGETLEEVKECIQDLRQNGVSMLTIGQYMQPTNTHYPVKEFIHPDIFTELKKFSYSLG
                     YKHVESGALVRSSYHADEQADFLKSLKNA"
     gene            1736..2032
                     /locus_tag="PLF66_12850"
     CDS             1736..2032
                     /locus_tag="PLF66_12850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64979.1"
                     /translation="MLKVNVFFDEESIRKSIYDSLATELPKIEGEICNYDIKQIILDM
                     AKKIIPYEIEWADAEKFGKNKMLIFHKNKVLQVDATELIESIRVLHQDYLEPQI"
     gene            complement(2029..3207)
                     /locus_tag="PLF66_12855"
     CDS             complement(2029..3207)
                     /locus_tag="PLF66_12855"
                     /inference="COORDINATES: protein motif:HMM:NF033165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein LipL45"
                     /protein_id="HMZ64980.1"
                     /translation="MNQISIVFLLCITTFFSQCKKPVDRIENKQEKKDNTPTALVLFS
                     VGESRIVHFDLTEERAALGSSFKTGDKITTGPKARVDIQINENAVIRLGANTKLDFVS
                     IAQNSSGSSDTKLQLTNGKIFANVKKENKNEDFVVSTPTVIAGVRGTSFILEVNREES
                     AVLKVVDGSVAVSPRVPIFEKIPLEEIDKNTGLKKINQTLQGAQVIIEKDQAIELPAN
                     DRVLASEKLDNKTIKEIISRLTDAANTKPESAEFTKNEQQELKTIVSVDPKLASEMIR
                     LNEDLSSGRIDEAKADELEKRRTALENQISNKQEVEKTKFNESIVVEPKKLQSNRDII
                     KYYERIEKIILINGKTEVGAIINQEDSIMVVHTENGIVRINTNEIIEVIYDYQTKYRF
                     "
     gene            3513..4430
                     /locus_tag="PLF66_12860"
     CDS             3513..4430
                     /locus_tag="PLF66_12860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64981.1"
                     /translation="MARKLLYNLNFIILFWIGTQFYHCSITQTQHRALQNFGDTALAM
                     STLSSNEMIAMRNATIQMNLNRIRIDSLSKPKSESPKDMSELKDLDGSFDLTTVSLRL
                     KAIASIRYYAKLLLSLSSDSSQKELDKATTNFLFSLENLSTEYKKMDEKKLNSIGEAI
                     RLFGGIFTEYKKKDSIKEIILNSREQIEILCDLLAKDFDPKSGKLATSFILTGEDLLL
                     AAEETLDPAEGKPERTKAKALLAYNLAQKNIQKKDTILPEIAKSILILKQANAELYET
                     FRSEVYTEKDIIKFVNTVKSVQDYKNILK"
     gene            4436..4732
                     /locus_tag="PLF66_12865"
     CDS             4436..4732
                     /locus_tag="PLF66_12865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64982.1"
                     /translation="MSKKEINYLDMLEGLKLLVEKAEGKEKAELRAKQKDFIKQVKKI
                     IALDIQADNEKYAQAIASLDKTNKDIETAIKDVNKISHVLTSLTHTMNVIGEFV"
     gene            4741..6288
                     /locus_tag="PLF66_12870"
     CDS             4741..6288
                     /locus_tag="PLF66_12870"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-like domain-containing protein"
                     /protein_id="HMZ64983.1"
                     /translation="MKVFVILLALTFCKITVDNKDLPPKFKYGGTEPAFEFPVNAEWV
                     NLKESISIEDLKGKITLLYFWKFTSIHCLQSLEEVKKLEKKWKKELIPIGIHAAKFTE
                     EKKKNSILNAIYKLDIKHPVLNDKDFFYSSKYNVNSWPYFVLIDPTGNVLGIHIGEGF
                     LEGFDKIIEGMVLEFQEKSLINFNPIQKLQTISFTSKSKLSFPTKIIFNQKGTEIFIS
                     DSHHNQVLRVDVETKKILDRIGSGEKGFKDGLFSAANFYSPQGMVLNDNLLYIADSKN
                     HSIRIANLKTKNVSTLIGNGEQAKGINVTGKGVSLISPSELILYKNKLYVTMQGAHQI
                     WTVDISNGEADIYAGQGNENLFDGSLQEASFAQPISITRDNVSFYITDAETSSIRKIN
                     YKSKEKVKTLIGRGLLEFGDINGKWEKAKLQYPMAITYSNGFLYLADTLNHKIKQVDL
                     LKEEITTLAGSGKEGADNGSSLEARFSSPEGIAVFKNKIFIADTNNHSIRFLDLETNI
                     VSNFDID"
     gene            6329..7027
                     /locus_tag="PLF66_12875"
     CDS             6329..7027
                     /locus_tag="PLF66_12875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016548728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HMZ64984.1"
                     /translation="MHFNRITKLYIVLSSIFLTFLLMAELTGSKLITAFGFVMTMGVI
                     PFPITFIVTDILNEFYGKKGVRFTTFLGMAMILLAYFLIVIDLQIPANQISPVDDISF
                     QKVFANSGLVIVGSITAYLIGQLIDIQVFHFIRTKTNGRFIWLRATGSTIISQLIDSY
                     VVIFIAFGKYEPSEVLGFPFYSLVNIPSLVSISTTNFVYKLLIAILLTPVIYISHTLI
                     ERYLGDEMIHLHHD"
     gene            7268..8689
                     /locus_tag="PLF66_12880"
     CDS             7268..8689
                     /locus_tag="PLF66_12880"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HMZ64985.1"
                     /translation="MADELEIFKKSLEIVDSIPSLSIESIEKYLKEIDTHTDGGLKAT
                     ILVIDDDKKIRDSLQTILEKKYNVILCENGQSGLQALTSSTFCVILDIKKSKDGFNTF
                     FEIKKRFLNLPVIFYSSLQHIKTPYDLLNEYQPFAYVVKEGDNRALLDTIAVAVVYYL
                     QINKNVLLIKQLQKMNQELEKSKKKYKDLVENSLDIIFSLDADGTIQSINKAASNILK
                     YNVKRLPGRKFSDLIYKTSIQNQVSLNEKVFQENFHALIESRDQVAFNCDMVTDYGEP
                     IEMHLKLKYIPSDDSFVIFGSAFNIEDDILLRLCETENQIYKIGNYLTQVDFICKRVS
                     KACSKYLDHDIAGELRLCLKELLVNSVEHGNLGITFEEKSESLANDEYFQVLINRQKD
                     PKNITKKVIVEYSLLADQVEFRITDMGDGFDHEKMLTRPRNDSTLSQVGHGRGIMMAR
                     EFFDKIEFNGKGNSVYLLKRFTR"
     gene            complement(8782..9975)
                     /locus_tag="PLF66_12885"
     CDS             complement(8782..9975)
                     /locus_tag="PLF66_12885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016550486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LeuA family protein"
                     /protein_id="HMZ64986.1"
                     /translation="MSNQTTRKPFFYDVTLRDGNQALRKPWNLLEKEIIFKQLVKLGV
                     NAIEVGFASASESDFNACEHLAKIAPENTVISSLSRAKNHEIDLSWAAIRHANQPRIH
                     IVYPVSSFAIQNMLKISNEEVIQNVTKAVSYARTLAGTKGSVQFSGEHFGDCIDTVEF
                     AIEVFKSAVKAGADVINLANTVERYRPMIFIQMIEEVVKELKDKAIISVHTHNDLGMA
                     TATTVESFFTGVTQIETSLNGLGERAGNTNLFEVACALYNCGESIDLNLNEIYPTAQL
                     VAKMSGIPIHEKAPLIGSDVFSHRSGIHQDGVNKTKHLKKSAYGAIDPTLIGRTDGHR
                     ITFTSQSGGGAIKSILSANGIHFSDEDISSLQPILKKHSDEIGRELVEEEVVQFAKKI
                     QKIVA"
     gene            10324..11532
                     /locus_tag="PLF66_12890"
     CDS             10324..11532
                     /locus_tag="PLF66_12890"
                     /inference="COORDINATES: protein motif:HMM:NF015400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HMZ64987.1"
                     /translation="MDKETKYDIVVFGATGFTGDLTAKYLAKKMKDESFRLGIAGRDE
                     IKLNRLKKELIAINPKCSEVGLVIADVKNYPSLLSMAKETKTVITTVGPYLKYGDSVV
                     KACIEGGADYLDLTGEGGFVNHVINTYDKKAKENNVKILNCCGFDSIPADLGTYYTVS
                     QLPSNEPIEVECFASFASTNTSTFGQFNSISGGTWHSAIGFMSLAELDRQKNSYTQIA
                     ASATNGRKVNPLPLQFRLREETKTYGAPLPVVDIEVVLRSAADIESYGPNFSYGHYAG
                     IHSTPLLIGGILGMGLLFSLAQFEPTRNFLLNLRKPGEGPDKEQRESNSFQLSFVGKS
                     RSKLVKTVVSGGDPGYGDTSKILGESALCLVLDRKEIPEKYGVITPAIAMRDKIIPRL
                     QKAGIQFKTL"
     gene            11578..12186
                     /locus_tag="PLF66_12895"
     CDS             11578..12186
                     /locus_tag="PLF66_12895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64988.1"
                     /translation="MKISLNSFTNFLHAVALASYTWLVYNGLIEKRPHYDIYILTCFA
                     LLFVIKIFGIIVHIPSIENDRNKHNFFWIIISILCIVLNFATLKSINTNLFLVSTGTG
                     IVAILAFLYIRSLFNSGGKFYLIASAFTLVHGACAIVTREKLSLAWFFLLMSNLLWIV
                     FSKIPYLHKHKYHNDIYHFMLIGSTYYLYSTIESGLWKSPIF"
     gene            12229..12402
                     /locus_tag="PLF66_12900"
     CDS             12229..12402
                     /locus_tag="PLF66_12900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64989.1"
                     /translation="MDMTLDQILIEIAKMDYIDQQSLATFLLDLVHTQEKMKEAMRQI
                     EESKESSRALMYP"
     gene            complement(12388..13587)
                     /locus_tag="PLF66_12905"
     CDS             complement(12388..13587)
                     /locus_tag="PLF66_12905"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016549011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HMZ64990.1"
                     /translation="MTQPIEQAIEEIRSGKMIILVDSEDRENEGDLVIAAEFADKDAI
                     NFMATHGRGLICIPMTEDRLSKLELARMVPTGGDHHGTAFTVSVDARHGVTTGISAAD
                     RAKTISTLIDEKTKPEDLMRPGHLFPLQAVKGGVLRRAGHTEASVDLCLLANLKPAGV
                     ICEIMNEDGSMARMKDLESFAKTHGLNIYTIEDLIRYRRTKDKLIHLEAEARLPTDYG
                     DFLIKAYTTEIDDKTHVALVKGEISKEDDVLVRVHSECLTGDIFMSNRCDCGPQLHAA
                     LMQIQKEGKGVLVYMRQEGRGIGLVNKLKAYAIQDTGLDTVEANEKLGFAPDLRDYGI
                     GAQILRDIGVEKMRIMTNNPRKLVGLEGYGLEITGRVPLEIQSVEENHNYLLTKKHKL
                     GHILGIH"
     gene            13715..14089
                     /locus_tag="PLF66_12910"
     CDS             13715..14089
                     /locus_tag="PLF66_12910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002973718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HMZ64991.1"
                     /translation="MELKLNNIGNVKVIEIYGKFDIENTEEFEALYNKQLESKPGILA
                     IDMNKLDYIDSSGIGCLIKSLNSIKNQKGVLILVGLKPMILNVFKLAKLDLFFQIMTN
                     DEFRNKYEDKDDSDIDELLGKR"
     gene            complement(14112..14717)
                     /locus_tag="PLF66_12915"
     CDS             complement(14112..14717)
                     /locus_tag="PLF66_12915"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010578545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HMZ64992.1"
                     /translation="MFTGLIETTGIILEKIPTNDGMIFQVEAILENNDLKLGDSISIN
                     GACQTVTELNSDKTKFKYYSSYKTLELTNFRNLEVGTKVNLERSVTYGARMGGHLVQG
                     HVDGVGVVRDRYAKDDGAVEVFLIDLPRNLCKYVIERGSIAIDGISLTVVSIQENTLE
                     LVLIPETIKKTNANLWNKGVIVNIETDLVARYVENMLLRKD"
     gene            complement(14720..15949)
                     /locus_tag="PLF66_12920"
     CDS             complement(14720..15949)
                     /locus_tag="PLF66_12920"
                     /inference="COORDINATES: protein motif:HMM:NF012601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase"
                     /protein_id="HMZ64993.1"
                     /translation="MNLPENFPEILDKLRRLSFQAMGYSSPNPPVACVITDLKGMILA
                     EAHTQRVGENHAEREAYKLFKEKYKNISEIKHLVFVTLEPCTHFGRTPPCLDLILEYK
                     PSCVYYGLADPNPLVRKRDGFLECSQRGIRIEQNLEIKKIASEFLTGFISRLEKNRPQ
                     FFIKSAISREGFYSNSERTRLSLSNIYSNQITQMLRAKLDAVIVGPKTVYRDLPRLDF
                     RGFISNRLEPSLENNITNFMDCLFYFSFEKDSLDYHNLHINEYQPYRIFILSLNQIPE
                     KEFFDAQSEISMRAKKSIFVLLDFDEKSDKHASLFQKLSEISLESPEILLNSQDLYPR
                     LIELFQKYSLNTVLIEGGNLLYSLFSKNFQERDSIYLIKTNKEIENGLRPEIGLISKK
                     KKYEKNIDTDTWEVYGE"
     gene            complement(16177..17127)
                     /gene="secF"
                     /locus_tag="PLF66_12925"
     CDS             complement(16177..17127)
                     /gene="secF"
                     /locus_tag="PLF66_12925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010569282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HMZ64994.1"
                     /translation="MFNINFSKYKYVSITFSILCIIAGFAYTFIVHKGFAHSLDFNGG
                     LRAVVVLEKGQGRPEIEKFFKEKEIEAIVIPLDKEKNHYQIDIGLGAVPKIEEYNNLN
                     KPKTSESGQTKSRVTIDELIFMLKHGFKLQEDRILSADQVGAVVGGELTTTGITLLFS
                     TLAIMTIYLSFRFQFKFALAASIALLHDLIFALAVIGAFQIKPSVPIIAALLTLFGYS
                     INDTIVIFDRIRENSNSKVKVSTPTMINMSINQTLGRTINTSFTTLISIVALIIGGAV
                     ELYDFAFVLTFGIIIGTYSSIFIAAPVIEIYNKIFHKETI"
     gene            complement(17120..19051)
                     /gene="secD"
                     /locus_tag="PLF66_12930"
     CDS             complement(17120..19051)
                     /gene="secD"
                     /locus_tag="PLF66_12930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016548924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HMZ64995.1"
                     /translation="MKSSSWVLLPITILVLSLVILFPNFAVRELELHISPSVASMTDQ
                     EKQELLNRFQERWKAEYNTQNALKIEPDPALGIPAQNYFLVTGRFITSAKINQISQEN
                     INLFLESKNNLRPTPIEKFFKNGKSMTIKLGLDLQGGMRVVMKGDFENYVSKLRDIYS
                     KEISDLKATIAANDKSKEEKDKATARLKDIEDSFILTESRKIIELEKAKLIIDNRLTN
                     QNLTEPQVRIQKDQDSIEVSLPGVANSSQILEILQNTETVEYRLEEPDGPNSYSQLIE
                     KAEQELMSQNKREETEIVQFQKVVASRPGKKVKDEFLFMLEEKYKIPRDKYKLYAYYA
                     RGNKPNSPLLPRRFIVLEKAIALSGNDLTDARPSFNSNNYGWTVSFSLTPAGTDKFLE
                     VTKNNKGRNLAIIWGDQVISNPRINEPIAGGHAEISGSFNQEDAIQLSNIISEGALPI
                     PLSVLEMRFIGPTLGIESIEVGLKSVMYGFILVILYMIFYYKLAGIVANISLIANLII
                     LMGLLSLMDFTLTLPGFAGIILTVGMAVDANVIIYERIKEELIAGKSLPIAVTQGFEN
                     AFWTIFDSNITTLISGILMIRLGNGPIKGFAITLCWGIITSMFTSLLLSRILLNLLVN
                     DIGIRKLKLGFRNYEVKNV"
     gene            complement(19079..19795)
                     /locus_tag="PLF66_12935"
     CDS             complement(19079..19795)
                     /locus_tag="PLF66_12935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ64996.1"
                     /translation="MKNFLRHTKTNQVLFNFSNAVTSVLRIFLTFLLSLSITFVVYAQ
                     DDDSDFLDKVLEDKTKKTDTKKVTQQTNPAAAKKKKPTKNGKKVPPAKTAAKTDPRKT
                     KPVPPVANTGEGNTVAHTPNLPQIQNNKLNLEYWVHEDLAMNPENIPGLEPAKTEVNP
                     PPAKTTASNVSEKPAEKKNPEKTIVDTKSNAIFNFINEYKKVIFIFAAIIIFAIYRLR
                     YVGPRDYNNSGRIFSKFRNK"
     gene            complement(19797..20120)
                     /gene="yajC"
                     /locus_tag="PLF66_12940"
     CDS             complement(19797..20120)
                     /gene="yajC"
                     /locus_tag="PLF66_12940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012388388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HMZ64997.1"
                     /translation="MYLLATFQYLIYLAQDAGGQSSSLSALILFPIMLVIMYFLVIQP
                     QRKEEKKKQEMIQGLVKGDSVVTTSGIHGKVVEFKDNNESVVLNVAKETNITFSTSAI
                     LKKKS"
     gene            complement(20186..21190)
                     /gene="trpD"
                     /locus_tag="PLF66_12945"
     CDS             complement(20186..21190)
                     /gene="trpD"
                     /locus_tag="PLF66_12945"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015676012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HMZ64998.1"
                     /translation="MNLNSLLQKSVAGNSLSEEEAYFLMISIMKGEVSDILLSSFLTS
                     IRMRVETADELVGFVRAMRDSCVKPSSGFNFDYLDTCGTGGDEKKSINISTLSALTLG
                     SMGVKIAKHGNRSVSSLCGSSDLLSALEYNVTVSHSEAESRLREKGFTFLFAPHWHPS
                     MKFAVETRKQLGFRTVFNILGPMSNPFSPPYQVLGVYTKELLPKVMHVLTKLGTKAAI
                     VCNSVDGFDEFSIFADTNYLLYQNGEVSTHLFSPDSLGLSSIQENEIFCNSKEEAVSL
                     SQRILSGEKLSGSHAVALNAGAALYLMEKAPNIKEGYHMAFDQLGSGKVMEFFQNLKN
                     "
     gene            complement(21187..21918)
                     /gene="pgsA"
                     /locus_tag="PLF66_12950"
     CDS             complement(21187..21918)
                     /gene="pgsA"
                     /locus_tag="PLF66_12950"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010575355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HMZ64999.1"
                     /translation="MEKSDFNLPNFLTVLRVLLVPFFIYFLIQDDFFFRLYALVIFLL
                     ASFTDLIDGYLARKWKQETEFGKFLDPLADKILVIGSFMTFILLDKQIELWMVLLIIL
                     RDMLITSLRWLAIQSGTSLHTTMMGKIKTAFQMGAIFVILVLFMAVSTKQRNVINSMY
                     EQGEASGKSGYQIAYENYSTFMNSPQEFTMNNFNWVDSAATFLPYYVMLITTIVTVFS
                     GLRYIFTNYELLNPFTIIKLIRRKN"
     gene            complement(21920..23254)
                     /locus_tag="PLF66_12955"
     CDS             complement(21920..23254)
                     /locus_tag="PLF66_12955"
                     /EC_number="2.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MiaB/RimO family radical SAM
                     methylthiotransferase"
                     /protein_id="HMZ65000.1"
                     /translation="MKSSVHEKSALKSFYITTLGCPKNTADSLHMEKSLLDEGLVPAK
                     SPEESDYHLINTCTFIQSATEETIDTILSAGKIKKQDEQRLVVVGCFAERYPKEIRSD
                     IPEVDLVFGTGRYSQAGKIIKEQFPQDFIHFKDVNDSLIDRDVLSTMQGFKPYAYVKV
                     SDGCNRGCHFCIIPSLRGEFRDQSEDRIYDDSRLAIRRGAKEICIVSQDTVFYGRDTD
                     KLKDVLTKITEIKGLELLRLLYLYPDKKTEKLLDLFISNPKIAPYLESPIQHVSERVL
                     KSMNRTGSFSFFRDLFTKARQVKDLEIRTSIILGYPGETADDVDEVLRFVEEVRPEKL
                     ALFAFSPQEGTKAAKLKLDVSKKEAARRVNLVRETHLNILKEIHLARVGKTYHAIVDS
                     VEDGIAEARRFQDAPEIDETVYIQNARLEPGQIGRVKINSFSEYDMDGTWEN"
     gene            complement(23257..24375)
                     /locus_tag="PLF66_12960"
     CDS             complement(23257..24375)
                     /locus_tag="PLF66_12960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012388384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4115 domain-containing protein"
                     /protein_id="HMZ65001.1"
                     /translation="MNTNKRVGQILREVREEKKLSVKDVSRDTNIAIKFIIALENEDY
                     AQFPAETFTVGFLKNYSDYLKLDTAGMINLYRGEQLVESQQPLEELTKPTVKMIALEL
                     KTNKFLPLGIILGICLAVFLVIFFSESGDTATNDEKPAEVTTDTPNPTSPLIPEISYI
                     SQSVPENSSVPFTLTPEQGFTFSVNNQQCKIFIKGVKKIDGENFASIGFNIFPEKKVY
                     TFDSKVGSETVLSYNNPELTSLRREIKIVTQAVTDNSAKILVRLLGDSKPDSNNKPIG
                     DVPIQVTLYFIRSSYVEFIIDGQTGERGLISSGETKQLEARDRLEIKVGDGGAVEMIQ
                     NGKDRVKLGKPGRLAKKIFLKVPNPYDNTQFIIKELGE"
     gene            complement(24398..25171)
                     /locus_tag="PLF66_12965"
     CDS             complement(24398..25171)
                     /locus_tag="PLF66_12965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004499376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HMZ65002.1"
                     /translation="MHFLIHKTTKNEQVLKNQTLFIKRFLFLISIFLFLFSNSIFAQA
                     HNWNSPSEVVKNIKKKFADLKSYTAEFKIQTVNNKKTTNMRGKTTYKAPGKIRYEFAE
                     PDGDLIVSDGKTLWIYIKKINAVGKQDLEINKQNTSGEPIFQSASPAGLNRLFRKYHY
                     KFDTISQPRNANNDGKSYFVLSLEQREKIGGFENMLLFVDAETYLIKKTIATDSRGKQ
                     TTIEFANMVLDPEVEDGTFNYHISGNSKIVNNPLVSDNQ"
     gene            25600..25977
                     /locus_tag="PLF66_12970"
     CDS             25600..25977
                     /locus_tag="PLF66_12970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015679641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HMZ65003.1"
                     /translation="MKLHTGIITKQIQESKKFYTEVLDFEIKFESEWFILLNVKNKPN
                     NELALMLPNQTQTRKTYFQEEYQKGIWFILEVESIKNYYEKIKTKKVKIDLELTEEEW
                     GDIHFTILDPNGIGIDIVEERKS"
     gene            26221..27084
                     /locus_tag="PLF66_12975"
     CDS             26221..27084
                     /locus_tag="PLF66_12975"
                     /inference="COORDINATES: protein motif:HMM:NF018184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HMZ65004.1"
                     /translation="MFQSYEKKYSSKIFPVHEYILENLDRKISLNQLAFLSGFSNFHF
                     HRIFKELQGEPIATHLKRLRLEKAAFELKISEIPLKEISIQAGFDSYEAFSRAFKKFF
                     LLSPQEFRKRNAISQKQIHHFSNLPKGIKLNEIHIRKIDSVKLAFLRKLGPYEDLPGP
                     DLNSKEFKMLLEFVKLNQMNPKNHLMIGVCQDDPEITKSNFVRFDIGFSITKKCKLLP
                     EMGIQTLKGGNYLVCRYRGEYKGLTEIYNFLLYEYCPKYKYKITSDPLFEVYIYGFEK
                     EIADIFIPLKK"
     gene            27552..27764
                     /locus_tag="PLF66_12980"
     CDS             27552..27764
                     /locus_tag="PLF66_12980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65005.1"
                     /translation="MPVALELDFKQLKTLIDQLNIEEKNELSKYLNNLTLSTRIKKLR
                     ASNKKVKISEQEIMSVVKEVKRKKAK"
     gene            27761..28162
                     /locus_tag="PLF66_12985"
     CDS             27761..28162
                     /locus_tag="PLF66_12985"
                     /inference="COORDINATES: protein motif:HMM:TIGR00305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HMZ65006.1"
                     /translation="MKIFIDTNIWISYFLSRSFGNLDSIFAKKDIEIFTCIEQMDELE
                     RVLRYPKLKNYFPEDVIADLLLFFSEKCTLVAIQRIVQECNDPEDNYILDIGIQHNLN
                     YIITGDSDLLSMHPFHKLSILKIQNFLHLIE"
     gene            28461..28949
                     /locus_tag="PLF66_12990"
     CDS             28461..28949
                     /locus_tag="PLF66_12990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65007.1"
                     /translation="MFEALDNDSSEIPKEAFLWILALYSLYFEFKISIDYESYDTLPP
                     MDDFFESEEIYQEELNRIDYKEKLTGDIEKFQKIFRKLLVKTYPDDLDKLSFFLNLDV
                     CWSEEEFIQFYTEMNESEITNTNDFCLVRDEKYNKYLNTVSIQNLEESKMMDGLAFVG
                     EL"
     gene            29298..29690
                     /locus_tag="PLF66_12995"
     CDS             29298..29690
                     /locus_tag="PLF66_12995"
                     /inference="COORDINATES: protein motif:HMM:NF023390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS/SEC14 domain-containing protein"
                     /protein_id="HMZ65008.1"
                     /translation="MEEIKFENSMAQVKYNKDLKIVEVHWKKSTVDEFKFQEVLNNGL
                     KELLENTTNKWLANTSLLGVIPQRTAEWVEKEWFPNVLKTVKKIKMAIIVPESMITKM
                     SIDKTIKTDVLEQQYFENLDEARNWLKG"
     gene            complement(30227..30667)
                     /locus_tag="PLF66_13000"
     CDS             complement(30227..30667)
                     /locus_tag="PLF66_13000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase"
                     /protein_id="HMZ65009.1"
                     /translation="MSLPVVFPPVSIGERVLIDGGVLNNVPDDLIRRKGADKILSVNV
                     SPLKDKTFYQLFDDKSISNKSLLRSLWEYIKYPPILKIMGRASMLEGREITKAKVSRM
                     DLFLHFHLEDFNLFDFSLYKDIIDKGESEAEKNFPEIKKLFLPE"
     gene            complement(30664..31956)
                     /locus_tag="PLF66_13005"
     CDS             complement(30664..31956)
                     /locus_tag="PLF66_13005"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding and patatin-like
                     phospholipase domain-containing protein"
                     /protein_id="HMZ65010.1"
                     /translation="MSPIEFRIRFLKKLKLFEGVQSKTLRKIALSLSEKKFHNKDIIY
                     FQGEISESLFIIRYGEVLVRVNNQKSIYLGPQEILGEVSVITKMQHSSTASASMDCLL
                     YELKGDLFLDLAETDRNLSRNLISFVSERFRDNLIVSKSSSMPRRLIAHVQMGIGEKF
                     SEEVRKVASVCEEVIVGKTRMISTEEFIGLSREKMIIKLSDLRSKSPVIHVLFKTPDK
                     IYEFENILIQADYILFYEQSIESGWTEKINTFQYLRNSLRNYEGRAIRYFQEKSISYR
                     ADVEKEKVYTNREFMARSIMARTRGLTLGGGGARALAHVGLLKVLERNKIQFDYVSGS
                     SMGAVIGALYARGESAENIENFVKKYFGGLDTPFDPTIPLISFYKGRKMKAMLKEIFG
                     DERLEDSKIPFVTSAIDLHAGEEYVFDKGPFWRLFFAQ"
     gene            32261..32986
                     /locus_tag="PLF66_13010"
     CDS             32261..32986
                     /locus_tag="PLF66_13010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65011.1"
                     /translation="MIVSFLLLIHALVNIENLTYKLKSSEPITIELHSLDPDTQKFIY
                     STLLEILNFYNKEDLFTAIYTALFESTMNCVKANAKKAFFQEKDLKVYDEFDFRSGTK
                     LFKKQVNDNNLPYYCSLAKKLGLTITINFIHSVKGLKVEILNNGHLFKEEENRIRTKL
                     ALGQKYVNIVEYHKDNSDKTEGEGLGLVVSILMLKEAGIPISDFRIGCKNGVTRTRIE
                     VPFTSSYISERQKYKMEFDRQSF"
     gene            complement(32983..34008)
                     /locus_tag="PLF66_13015"
     CDS             complement(32983..34008)
                     /locus_tag="PLF66_13015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012390271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HMZ65012.1"
                     /translation="MAQNPIKGISKMGFQWETRDPFLFCVHHEDYYPKGNSSMGPANQ
                     YLQGRNIGMDFEVKDGFRMYHGEKIPGFPNHPHRGFETVTVVRSGFVDHSDSMGASGR
                     YSAGDVQWMTAGAGVQHSEMFPLIKEDEENKLELFQIWLNLPRVNKFVKPHFTMLWNE
                     SIPRYTAKDTDGKKIEVEIIAGSLGGMKAPSPPPDSWAADPKNQVAIWIIRLESGAKW
                     TLPASNSSVNRMLYFFRGKSLSVAANTVMPYNNIDLISDQDVLLENGEGEAQLLLLQA
                     KPIGEPVVQYGPFVMNTRQEIQDAFNDFQATHFGGWSWTSSEPVHDRKKGRFAKHADG
                     KEEFPNA"
BASE COUNT        11560 a         5935 c         5400 g        11281 t
ORIGIN      
        1 gaacttcttg tccccgaatt tgcggtctcc gaatttttta tcttcgaact tcttgtcccc
       61 gaatttgcgg tctccgaatt ttttatcttg aaactttcga ttttcgaact ttcgattgcc
      121 tatatcgtct ctttcttgtc tctcaaatct aggcttgcga tttcctatat cgtcttgtat
      181 ttctctctct ggttgaaacc gatttccaga cataggatta cggttgtttg cccctttact
      241 ccaatatcta cccctctcct tatcgccccc tccctccgat tgatttctgt ccctgaaaga
      301 aaactttttt ccactatttg gtcttgtatt tgatttgaaa cggttttcag aaaaactctc
      361 gctctcgcca tttgttttaa attggttgga tttgggtctt ctgtcatttt ttttgaatgt
      421 atttctttta cttccaaacg aattgtcttc aaaatctctc gaaaattttc tatttttgga
      481 aaaattagac tcagagtcct tatttttttg aaatccaaag aaattcttct cttctgtatt
      541 ctttttaaaa cgtctttctt cttgatttac gtctctggta aatttattat tgactggctt
      601 ataattcttt tttttactag aatcatggaa tttcttcttg gactctttta tattcttgtt
      661 ctcattcata gtttaaactc ctatccagtt tctgaaatta gattctcatg caaagagatt
      721 tattctattg atttcaaaaa taaacgaatg actatggtat aaccgactat gaatccacta
      781 aaaaaaaagc ctagaactaa aaataccagt ccaatgggag aaaaaccaga gtggctaagg
      841 gtcaaagtaa aatttccaac agaacaagat cctgtctcta gcgtccgtaa gaatctcaca
      901 aatacaagat taaacactgt ttgcgaaagt gcttcttgcc ctaacttgaa taattgctgg
      961 tctagaaaaa cggcaaccta tatgctcgca ggagatattt gcacaagaaa atgctcctac
     1021 tgtgatgtag cctccgggaa acctcttcca cttgacaaag aggagccaat ccgcattgcc
     1081 aattcagtaa gagaattagg acttcgttat gttgtgatta cagccgttaa ccgcgatgat
     1141 ttaaaagatg gaggtgcaat gcattttcat cagactgttc aggaaattag aaaacaaagc
     1201 ccagaatgta gaattgaact attaattcca gatttaaaag gaaaaagaga atctttagat
     1261 cttatttact ctgcaaatcc ggatattatc aatcacaata ttgaaacagt aaaaagtctt
     1321 tttaaagaag tggctcctgc taaaaactat gaaacatccc taagcgtctt aaaggatatt
     1381 tctgaacatg gatttattac aaagagtggt atcattttag gacttggcga aacactagaa
     1441 gaagtaaagg aatgcatcca agacttgcga caaaatggtg tttcaatgct tacaatcggt
     1501 caatatatgc agccgactaa cacacattac ccagtaaaag aatttataca ccccgatatt
     1561 tttacagaat taaaaaaatt ttcctattca cttggttata agcatgtaga atcaggagca
     1621 cttgttagaa gttcttacca tgcggatgaa caggcagatt ttctaaagag tctaaaaaat
     1681 gcataaaaaa gagtttttaa tattcataag aaaggtttct ttctgaggta aggctatgct
     1741 aaaggtaaat gttttttttg atgaagaaag tatccgcaaa tctatttatg attcgcttgc
     1801 aactgaattg cctaagatag aaggagaaat ttgcaattat gatataaagc aaattattct
     1861 agatatggca aaaaaaatca ttccttatga aatagaatgg gctgatgcag aaaaattcgg
     1921 aaaaaataaa atgctaatct ttcataaaaa taaagttctg caggtagatg ctacagaact
     1981 tattgaatca attcgtgtat tacatcaaga ttatctagaa ccacaaatct aaaagcgata
     2041 ctttgtttga taatcgtaga tcacttcaat gatttcattt gtattgatcc gaacgattcc
     2101 attttccgta tggacaacca taatggagtc ttcttgatta ataattgccc caacttctgt
     2161 tttaccattt attaaaatta ttttttcaat ccgttcatag tatttaataa tatctctatt
     2221 ggattgcagt ttcttaggtt caacgactat cgattcgtta aactttgttt tttctacttc
     2281 ttgcttattg gaaatttgat tttcgagagc agttcttctt ttctctaact catctgcttt
     2341 tgcttcgtca attcttccgc tacttaaatc ctcattgagt cgaatcattt cactggcaag
     2401 ttttggatcc acagaaacaa tcgttttgag ttcttgttgt tcattttttg taaattctgc
     2461 agattccggt tttgtgtttg cagcatccgt tagtcgcgaa attatctctt taatagtctt
     2521 attatctaat ttttcggaag ctaagactct atcgtttgca ggaagctcga ttgcttggtc
     2581 tttttcaata atcacttgag cgccttgtaa agtttggttg atcttcttga gtcctgtatt
     2641 tttatcaatt tcttctaatg gaattttttc aaatattgga actcgtggag agacagctac
     2701 cgacccatcc actactttca aaactgcact ttcttcccga tttacttcca aaataaaaga
     2761 agttccccga acacccgcaa tgactgtagg tgtagagaca acaaagtctt catttttatt
     2821 ctcttttttc acattcgcaa agattttccc atttgtaagt tgtaattttg tgtcggaact
     2881 tccagaagaa ttttgagcaa tcgaaacaaa atctagcttt gtatttgcac ctagtcggat
     2941 cactgcattc tcattaattt gaatgtccac tctagctttt ggtcctgttg taattttatc
     3001 gcctgtttta aaagatgagc ctaaagcagc tctttcttct gttaaatcaa aatgaacgat
     3061 tctagattct ccaacactaa aaagaaccaa tgctgtaggt gtattatctt ttttttcttg
     3121 tttgttttca attctatcaa caggtttttt gcactgagaa aagaaggtag tgatacaaag
     3181 tagaaaaaca atagagatct gattcataaa aaagatttcc tcgcaatata tgtaaattca
     3241 ttactacaaa caggcattat acaaaaagaa gtgaaaggta aagctaataa ttttggaagc
     3301 ctatgaaaat cgaatcttaa agcttttgaa ggaatctgca atctagagct tgtgatggaa
     3361 accagattgc atgacacatt cccttgtata aggcaagtca ttaaaacatc ttccaacact
     3421 attcagaaag ttaaaagcaa tttttttcaa aataagtcta ttttcgtttg tatttagttt
     3481 gcaaagaaat aaatgttcct tcaggagaga ttatggctcg taaattatta tacaatttga
     3541 attttataat tttattctgg attggaaccc aattctatca ctgttccatt acacaaacac
     3601 agcatagagc attacaaaac ttcggagaca cagcactcgc tatgtcaaca ttaagcagca
     3661 acgaaatgat cgccatgcga aacgcaacta tccaaatgaa tctcaatcgt attcgcatcg
     3721 atagtctatc caagccaaaa tcagagtcac ctaaagatat gtctgaactg aaagacttag
     3781 atggatcgtt tgatcttact accgtttctc ttcgtttgaa ggcaattgct tccattcgat
     3841 attacgcaaa attattatta tccctttcat ctgattcttc ccaaaaagaa ttggataagg
     3901 ctactactaa ttttttattt agtctggaaa atctttccac tgaatataag aaaatggatg
     3961 agaaaaaact caactcaatc ggagaggcaa ttcgactctt tggaggaatc tttactgaat
     4021 acaaaaagaa agactcaatt aaagaaatta ttttaaactc aagagaacaa attgaaattc
     4081 tatgcgattt gcttgcaaag gattttgacc ccaaaagcgg taaattagca acctccttta
     4141 ttttaacagg agaagattta ttactcgccg cagaagaaac tttagatccc gccgaaggta
     4201 agcctgagcg gacaaaagca aaagcacttc ttgcttacaa tttagctcaa aagaatatac
     4261 aaaaaaaaga tacaattctt cctgaaattg caaaatctat tcttattctg aagcaagcaa
     4321 atgcagagtt gtatgaaaca tttcgctctg aggtctatac agagaaagat ataattaaat
     4381 ttgttaacac tgtcaaatca gttcaagatt ataaaaatat tttaaagtag gatatatgag
     4441 taaaaaagaa atcaattatt tagatatgct agaaggtctc aaattacttg tagaaaaagc
     4501 agaaggaaaa gagaaagcag aactgagagc aaaacaaaaa gattttatta aacaagtcaa
     4561 aaaaatcatt gctcttgata tacaagcgga taatgagaag tatgcgcaag caatcgcaag
     4621 cttagacaaa acaaataaag acattgaaac agctatcaaa gatgtaaata aaatttcaca
     4681 tgttctaacc tctctcacgc acacgatgaa tgttattgga gagttcgttt aaacttaaaa
     4741 atgaaagtct ttgttatact acttgctttg accttttgca aaataacagt agataacaaa
     4801 gatttacctc caaaatttaa atacggagga acagaaccgg ccttcgagtt tcctgtcaat
     4861 gcagaatggg ttaatctaaa agaatccatt tctattgaag atttaaaagg aaaaataacc
     4921 ctactctatt tttggaaatt tacatctatt cattgcctgc aaagtttaga agaagtaaaa
     4981 aaattggaaa aaaaatggaa gaaagaactg attccaattg gaattcatgc ggctaaattt
     5041 acagaagaaa aaaagaaaaa ttctattcta aatgcaatct ataaactcga tataaaacat
     5101 ccagtattaa atgataaaga ctttttctat tctagtaaat acaatgtaaa ttcttggccc
     5161 tattttgttt taatagatcc aacaggcaat gttttgggaa ttcacatagg agaaggtttc
     5221 ttagaaggtt ttgataaaat cattgaagga atggttttag aatttcagga aaaaagccta
     5281 atcaatttta atccaattca aaaactacaa acgatctcct ttacttctaa atccaaatta
     5341 agttttccta caaaaataat ttttaaccaa aagggaacgg aaatttttat ttcagattca
     5401 catcacaacc aggttttacg agtggatgta gaaactaaaa aaatcctaga tcgaattggt
     5461 tcaggagaaa aaggatttaa ggacggttta ttttcagcgg ctaatttcta ttctccacaa
     5521 ggtatggtat tgaatgacaa tttactctac atagcagatt ctaaaaatca ctccattcgt
     5581 attgccaatt taaaaacgaa aaatgtctct acattgatcg gaaatgggga acaggcaaaa
     5641 gggatcaatg taacaggaaa aggagtttct cttatttcac ctagcgaact aatcctttat
     5701 aaaaacaagt tatacgtaac gatgcaaggg gcacatcaaa tctggactgt tgacatttca
     5761 aatggagaag cagatatcta tgctggacaa ggaaatgaaa acctatttga cggaagtttg
     5821 caagaagcat ctttcgcaca accaatctct atcacaagag acaatgtttc cttttatatc
     5881 acagatgcag aaacaagctc aatacgtaaa ataaattata agtcaaaaga gaaagtgaaa
     5941 accttaatcg gtcgagggct cttagagttt ggggatataa acgggaaatg ggagaaagca
     6001 aaactgcaat acccaatggc tattacttat tcgaatggtt ttttatatct agccgatacg
     6061 ttaaaccata aaataaaaca ggttgatctt ctaaaagaag aaataacaac tttagcagga
     6121 agtggaaaag aaggagcaga taacggttct tctttagaag caagattttc aagtccggaa
     6181 ggaattgctg ttttcaaaaa caaaatcttt atcgcagata cgaataatca ctcaattcgt
     6241 ttccttgact tagaaacaaa tatagtttcc aattttgaca ttgattaata tattataaaa
     6301 agcctaattt ttaggtaagg aatttaagat gcatttcaat agaatcacaa agttatatat
     6361 tgtcctatcg agtatatttt taacctttct tctaatggcg gaattgacag gcagtaaact
     6421 catcacagcc tttggattcg ttatgacaat gggtgtgatt ccatttccta tcacttttat
     6481 tgtgacagat attctaaatg aattttatgg aaagaaagga gtccgattca caacctttct
     6541 aggaatggca atgatcctac ttgcttattt tctaatcgta attgatctac aaattcctgc
     6601 gaatcaaatt tctcctgtag atgatatctc atttcaaaag gtgtttgcaa actcaggtct
     6661 tgtgatcgta ggatcgatca ctgcttatct catcgggcaa ttaattgaca ttcaagtttt
     6721 tcatttcatc agaaccaaaa caaatggaag gtttatttgg ttaagagcaa ctggctctac
     6781 catcatctct caattgattg attcttatgt agttatattt attgctttcg gtaaatatga
     6841 accaagtgaa gttttaggat ttccatttta ctccctggta aatataccta gcttagttag
     6901 tatttctact actaattttg tatacaaatt gctaattgcg attcttctta cacctgttat
     6961 atatatatcc catactctca tcgaacgtta ccttggagat gagatgattc atttgcacca
     7021 tgactaattg aaaatcaaaa atagaatcca atgatttaaa aatagatcgg aaagaaagat
     7081 gaatagaaat ttccaaatca aagtaaatgt gtaatggagt cagtttttcc tactgactgg
     7141 attcattgaa tagatagaaa ttcaaactgt tgactaccat tccgttttaa agaaatattt
     7201 caattacaga aaatctaatt gtaaaaaacc atagaattag gtaagactgg ttttattgga
     7261 tggttgtatg gcagacgaat tagaaatctt taaaaagtct ttagaaatag tagatagtat
     7321 cccctcactt agcattgaaa gcatagaaaa atatctaaaa gaaattgata cacacacaga
     7381 tggaggttta aaagctacaa tactagtcat tgatgatgat aaaaaaattc gtgactcatt
     7441 acaaacaatt ttagaaaaaa aatacaatgt aattctttgc gagaatggac agagcggatt
     7501 acaagcatta acctcttcta ccttctgtgt aattttagat ataaagaaaa gtaaagatgg
     7561 atttaatact ttttttgaaa ttaaaaagcg tttcttaaat ttacctgtta ttttttattc
     7621 ctcacttcaa catattaaaa ctccctatga tcttttaaat gaataccaac cttttgctta
     7681 tgtggtaaag gaaggagaca atagagcact cttagatacg attgcagttg cagtagtcta
     7741 ttatttacaa atcaataaaa atgtgcttct aatcaaacaa cttcaaaaga tgaatcagga
     7801 attagaaaaa tccaagaaaa aatacaagga tttagtagaa aactcactcg atattatctt
     7861 ttctctagat gcagatggaa caattcaatc tataaacaaa gctgcatcca atattttaaa
     7921 atacaacgtt aaacgacttc caggaagaaa gtttagtgat cttatctata aaacttctat
     7981 acaaaatcaa gtaagtttaa atgaaaaagt atttcaggaa aattttcatg cactgattga
     8041 gtctagagac caagtcgcct tcaactgtga tatggtaacc gattatgggg aaccgatcga
     8101 aatgcattta aaattaaaat acatcccgtc tgacgatagc tttgtaatct ttggttcagc
     8161 atttaacata gaagatgata ttttactaag gctttgtgaa acagaaaatc aaatttataa
     8221 aattggaaat tacctcacac aagtcgattt tatttgtaaa agagtctcca aagcatgttc
     8281 caaatattta gaccatgata tagctggaga attacgactt tgtttaaaag aacttttagt
     8341 taattcagtt gagcatggaa atcttggaat cacctttgaa gaaaaatccg aatctctcgc
     8401 gaacgatgaa tactttcaag ttttaatcaa cagacaaaaa gatccaaaaa atattacgaa
     8461 gaaagtaatt gtagaatact cattattagc cgaccaagta gaatttagaa tcacagatat
     8521 gggagatgga tttgatcatg aaaaaatgtt aactcgccct agaaatgatt caactctttc
     8581 tcaagtaggt catggaaggg gtattatgat ggcaagagag ttctttgata aaattgaatt
     8641 caatggaaaa gggaattctg tttacctatt aaagagattt actcgttgaa atttatatcc
     8701 caaaaataaa tctaatagaa taaaaaatcc actgaagagg aaactggagt ttcagcatct
     8761 atcagtggtt tttttaatat cttaggctac tatcttttga attttctttg caaactgaac
     8821 gacctcttcc tctactaact cgcgcccaat ttcatctgaa tgttttttca aaattggttg
     8881 caaggaagaa atatcctcat ctgaaaaatg aattccattg gcagataaaa tcgatttaat
     8941 cgcaccacca ccggattgac ttgtaaacgt tatacgatgt ccgtcggttc ttcctatcaa
     9001 tgttggatca atagcaccat atgcagactt tttcaaatgc ttcgttttat tgactccgtc
     9061 ttgatgaatt ccgcttctat gagaaaagac atcacttcca atcaggggag ctttttcatg
     9121 gattggaatt ccggacattt tagcaaccaa ttgtgcagta ggatagattt catttaaatt
     9181 taaatcaata ctttccccac aattatacaa agcacaagca acttcgaata aatttgtatt
     9241 tccagctctc tcccctaacc catttaaaga tgtttcaatt tgcgtaactc ctgtaaaaaa
     9301 gctttctaca gttgttgctg ttgccatacc taaatcattg tgagtatgga cagaaataat
     9361 cgccttatct tttaattctt ttacaacttc ttctatcatc tgaataaata tcataggacg
     9421 atatctctcc actgtatttg ctaaattaat tacatcagct cctgctttta cagccgattt
     9481 aaaaacttca atagcaaatt ctactgtatc gatacaatct ccgaaatgtt ccccgctaaa
     9541 ttgcacactt cccttagttc cagctaacgt tcttgcatag ctgactgctt tggttacatt
     9601 ttgaattact tcctcattcg aaatctttaa catattctga atcgcaaaag aactcacagg
     9661 atacacaatg tgtattctag gttgatttgc atggcgaatt gccgcccaag acaaatcgat
     9721 ttcatgattc ttagcacgag aaagacttga tattacagta ttttcgggag caatttttgc
     9781 taaatgctca caggcattaa aatcactttc actagcagaa gcaaatccaa cttcaattgc
     9841 attgactcct agcttaacta attgtttgaa gataatttcc ttttcgagta agttccaagg
     9901 ctttcgcaaa gcctgatttc catctcgtaa agtcacatca taaaagaaag gttttcgggt
     9961 tgtttggtta ctcattgtta ttctcctttt tttccactca tttcgatcgg gctaggagtt
    10021 tacagatagt atccacaaac aaaaaaagcc cgctcggttg gagtgggctt ttcatttcac
    10081 aacttattgc tcacgccaac ctacataagt aggagaagga ggagcggaag aagtgtgaat
    10141 aaaatttgat tcatactttc ttcatcggaa atttgagaaa tgttgtcaag tagaaattga
    10201 aatttatttt tcaaatctga ttttaaagtt ttattctttt ttaatacaaa atctcttttc
    10261 atttcattta aaaaaattga ccgtctgaat ttaaaaatcc gcaatgaaga aaagaaggtg
    10321 atcatggata aagaaacaaa gtatgatata gtagtctttg gagctacagg atttacaggc
    10381 gatttaactg caaaatatct cgcgaagaaa atgaaggatg aatcctttcg tttaggaatt
    10441 gctgggcgag atgagattaa attaaatcga ctaaagaagg aactaatcgc gattaacccc
    10501 aaatgcagtg aagttggttt agtgatagca gatgtaaaaa attatccaag tttactttca
    10561 atggcaaaag agacaaagac agtcattaca acggtaggac cttacttaaa gtatggagac
    10621 tctgtagtta aagcctgtat cgaaggcgga gctgactatt tagatttaac tggcgagggt
    10681 gggtttgtaa accatgtaat aaacacatat gacaaaaaag caaaagaaaa caatgtaaaa
    10741 attctaaact gttgtggttt tgattccatt ccagcagatc ttggaaccta ctatactgta
    10801 tcgcaattac cgtctaatga accaatagaa gtagaatgtt tcgcaagttt tgcgagcaca
    10861 aatacctcaa cttttggtca gtttaattct atttctggag gaacttggca ttctgcaatt
    10921 ggatttatga gtttagctga gttagacaga caaaaaaact cttacactca gattgcagct
    10981 agtgcaacta acggtagaaa ggtaaatcca ttgccattac aatttagatt gagagaagaa
    11041 acaaaaacct atggagcgcc actcccagtt gtagacattg aagttgttct tcgctctgca
    11101 gccgatatag aatcctatgg acctaacttt tcttatggac attatgcagg aattcattct
    11161 accccacttt taattggagg gattttagga atgggtcttt tattctctct tgctcaattt
    11221 gaaccaactc gaaattttct tttgaattta agaaaacccg gggaaggacc tgacaaagag
    11281 caaagagaaa gcaattcgtt tcaattgtct tttgtaggaa aatcgagatc caaacttgta
    11341 aagacagtag taagcggagg cgatcctgga tatggtgata cttctaaaat tttaggtgaa
    11401 tccgctcttt gtttagtttt ggatcgaaaa gaaattcctg aaaaatatgg agttatcact
    11461 ccagcgattg caatgcgaga taaaatcatt ccaagattac aaaaagcagg aattcaattc
    11521 aaaacattgt aatcgatttt ctatttacaa gaatcaaaag agaaattttc tagaagcatg
    11581 aagatttctc tgaattcatt tacaaatttt ctacatgcag tagcgcttgc atcttacaca
    11641 tggcttgtat ataacgggct cattgaaaaa agaccccatt acgatatata catactaacc
    11701 tgttttgccc ttttattcgt aatcaaaatt tttggaatca tagtccatat accttccatt
    11761 gaaaatgatc gaaataaaca taacttcttt tggatcatta tttcgattct atgtattgtt
    11821 ttaaactttg ctacattgaa atcaatcaat acaaatctat ttttagtttc cactggaaca
    11881 ggaatcgtag caattcttgc ctttttatat atccgatccc tttttaattc tggaggaaaa
    11941 ttttatctga tcgcttccgc ttttaccttg gtgcatggcg catgtgcaat tgtgaccaga
    12001 gaaaagttaa gtcttgcttg gtttttttta ttaatgtcta acttgctctg gattgttttt
    12061 tcaaaaattc cttatttaca taaacacaaa tatcacaatg acatttatca ttttatgtta
    12121 atcgggagca cctattatct ctacagcaca attgaatctg gtctatggaa aagtccaatt
    12181 ttttaaaaac aagttgactt ttaattttca tttcacaaat tttcttctat ggatatgacc
    12241 ctcgaccaaa tactcatcga aatagctaag atggactata tagaccaaca gagcttagct
    12301 actttccttt tagatttagt gcacacacaa gaaaagatga aagaagctat gcgtcagata
    12361 gaagaaagca aagagtcatc tagagcctta atgtatccct aaaatatgac ctaatttatg
    12421 tttcttagtt aaaagataat tgtgattctc ttccacagac tgaatttcaa gaggaactct
    12481 ccctgtaatt tccaatccgt atccctctag tccaactaac tttcgggggt tattcgtcat
    12541 tatccgcatt ttttcgacac ctatatcacg cagtatctgt gcaccgattc catagtcgcg
    12601 tagatcagga gcaaagccta atttttcatt tgcttctact gtatccaaac ctgtatcttg
    12661 gattgcatag gcttttaatt tattcactag accaatccca cgcccctctt gtctcatata
    12721 aactaaaaca cctttacctt ctttttgaat ttgcatcagt gctgcatgaa gctgtggtcc
    12781 acaatcacat cgattgctca tgaagatatc accagtcaaa cactcagaat gaacacgaac
    12841 taaaacatca tcctctttcg atatttctcc cttcacaagt gccacatgag ttttatcgtc
    12901 tatctcagta gtgtaagctt tgattaagaa gtcaccataa tcagttggaa gtctagcttc
    12961 tgcctctaag tgaatcaatt tatcttttgt tctacggtag cggattaaat cttcaatggt
    13021 gtagatattt aaaccatggg tcttcgcaaa gctttccaag tctttcattc gagccatact
    13081 accgtcttcg ttcataatct cacagattac acctgctggt tttaaatttg cgagtaaaca
    13141 taaatcaaca gacgcttccg tatgcccagc tcttcgcaat accccacctt taactgcctg
    13201 aagaggaaat aaatgtccag gtctcattaa atcttctggt tttgttttct catcaattaa
    13261 ggtagagatt gtcttcgctc gatctgctgc agaaattcca gttgtaactc catgtcttgc
    13321 atctacagat actgtgaatg ctgttccatg gtgatcgccg ccggtaggca ccattctagc
    13381 taattctaat ttacttagtc tatcttccgt cattggtata cagatgagtc ccctgccatg
    13441 ggttgccata aaattaattg catctttatc tgcaaactca gctgctatca ctagatcccc
    13501 ttcgttttct ctgtcctcag agtcgacgag aataatcatt ttaccacttc taatttcttc
    13561 gattgcttgc tcaataggtt gtgtcattct ataaccattt catttaggaa agagttttaa
    13621 actataaaaa aatactattg attttatata tgtaataatt atgattagtc aaacaggttg
    13681 gtttttaaag ctgattgtct gaaaggatac aggaatggag ctaaagttaa ataatatagg
    13741 caatgtcaaa gtaattgaaa tatacggaaa atttgacatc gaaaatacgg aagaatttga
    13801 agcactttat aataaacaat tagagtcaaa gccaggcatt ctagctattg atatgaacaa
    13861 attggattat attgattctt ctgggattgg atgtttgatt aaatctctca atagcataaa
    13921 aaatcaaaaa ggagttttaa ttctcgtggg tttaaaacct atgattttaa atgtttttaa
    13981 attagcaaaa ctagatttat tttttcaaat tatgacaaat gatgagttca gaaacaaata
    14041 cgaagacaaa gatgattctg atattgatga actcttaggc aaaagataaa caaaaagcgg
    14101 gtattacccg cttaatcctt tcttaataac atattctcca cataacgagc tactaaatca
    14161 gtttcaatat ttacgattac gcctttattc catagattgg cgttggtttt ttttatagtt
    14221 tcaggtataa gaactagttc taaagtattt tcttgtatag atacgacagt aagtgatata
    14281 ccgtctatcg caatactacc tcgctcaatg acatacttac ataaatttct agggagatca
    14341 atgagaaata cttccactgc tccatcatct ttcgcataac gatctcttac aacacctact
    14401 ccatccacat gtccttgaac cagatgcccg cccattctag ctccgtatgt aacagatctt
    14461 tctaaattta cttttgttcc aacttctaaa tttctaaaat tggtcagttc aagagtttta
    14521 taggaagaat agtatttaaa cttagtttta tcagaattta attcggttac ggtctgacag
    14581 gctccattga ttgaaataga atctcctaat ttcaaatcat tattttctaa aatggcttct
    14641 acttgaaaga tcattccatc gtttgttgga atcttttcta aaatgattcc tgtcgtttca
    14701 attaatcctg taaacatttt tattctccgt atacttccca agtatctgta tcaatatttt
    14761 tttcatattt cttttttttt gaaattaaac ctatctctgg tcttaatcca ttttcaattt
    14821 ctttatttgt tttaatgaga tagatggaat ctctttcctg aaaattcttt gaaaagagtg
    14881 aatagagtaa attcccacct tcaattaaaa cagtattcaa agaatatttc tgaaataatt
    14941 ctattagcct aggatatagg tcttgagagt tcaggaggat ttccggtgac tctaaagaaa
    15001 tttcagaaag tttctgaaat agagaagcat gtttgtctga tttttcatca aagtctaata
    15061 aaacaaaaat tgattttttg gctctcattg agatttcaga ttgtgcatca aaaaattctt
    15121 tttctggaat ttggtttaaa ctcagaataa aaattctata aggctgatac tcgtttatat
    15181 gcaagttatg ataatctaaa ctatctttct caaaactaaa ataaaataaa caatccataa
    15241 aatttgttat attattctct aaacttggtt ccaatcgatt cgaaatgaat cctctaaaat
    15301 ctagtcgtgg taaatctcga taaactgttt ttggaccaac aatcaccgca tctaattttg
    15361 ctctaagcat ttgagtgatc tgatttgaat agatattcga caggctcaat cttgttcttt
    15421 ccgaattaga ataaaaacct tctctggaaa tagcagactt aataaaaaat tgcggtctgt
    15481 ttttttctag tctagaaata aaaccagtta agaattccga ggcaatcttt ttgatttcta
    15541 gattctgctc tatcctgatt cctctttgac tgcattctaa aaaaccatct ctttttctta
    15601 caagtggatt tggatcggct aatccatagt aaacgcaaga gggtttatac tcaaggatta
    15661 gatctaaaca aggaggagtt ctaccgaaat gagtgcaagg ttctagagtt acaaaaacaa
    15721 gatgctttat ttcagaaata tttttgtatt tttctttgaa aagtttatag gcttctctct
    15781 ctgcatgatt ttctcccact ctttgcgtat gagcctccgc taaaatcata ccttttaaat
    15841 cagtgattac acacgcgaca ggaggattag gagaagaata acccatcgcc tgaaacgata
    15901 ggcgtcttag tttatctaaa atttctggaa agttttcggg tagattcatc aaatataaag
    15961 agtaagacaa ctagatcgca tttcaaaaaa agatatttta aaaggttata ggatcaggga
    16021 taaataggaa gatttttcaa tccatctttt catctgtggg ttaaaaagtt cttatatcga
    16081 actaacgtta attaccaccg ttcgagccat ctcttttatc gatgatgatc ggtggtaatt
    16141 ttttttactt tacttaaagt atggaaacaa tttcatctag atcgtctctt tatgaaatat
    16201 cttattgtaa atttcaataa ccggtgccgc aataaagata gaagagtaag ttccaataat
    16261 aattccaaat gttaaaacaa aggcaaaatc ataaagttct actgctcctc caataatcaa
    16321 tgcaacgata gatatcaaag ttgtaaaaga agtgttaatg gttctaccca aagtttgatt
    16381 gattgacata ttaatcatag ttggagtaga aactttcact ttagaatttg aattttctct
    16441 gattctatcg aaaatcacga ttgtatcatt gattgagtat ccaaaaagag tcaagagtgc
    16501 tgcaataatt ggcacacttg gttttatttg aaaagcccca ataacagcta acgcaaaaat
    16561 taaatcatgt aagagtgcaa tcgatgctgc aagtgcaaac ttaaactgaa aacggaagct
    16621 gagataaata gtcatgattg caagagtaga aaaaagtaag gtgatacctg tagtcgttaa
    16681 ttccccgcca actacagcac ctacttgatc tgcacttaaa attctatctt cctgcaactt
    16741 aaatccatgc tttaacatga aaatcaattc atcaattgta actctagact tagtttgtcc
    16801 tgattctgaa gtctttggtt tgttaagatt attatactct tcaatctttg gaactgctcc
    16861 aagacctata tcgatttggt agtgattttt ttccttatca agcggaatca caatcgcttc
    16921 gatttctttt tctttaaaga atttttcaat ctcaggtcta ccctgacctt tctccaaaac
    16981 aacaactgct ctaagtcctc cattgaaatc aagagagtga gcgaatcctt tatgcacgat
    17041 aaacgtatag gcaaatcctg ctataataca aaggattgag aatgtgatag atacatattt
    17101 atattttgaa aaattaatgt taaacatttt tcacctcgta atttctaaaa cctagtttca
    17161 gtttcctaat tccaatatca tttactaaca ggttcaaaag aattctgcta agtaaaaggg
    17221 aggtaaacat agaagtaata atcccccaac aaagtgtaat cgcaaatcct ttgatcggtc
    17281 cgttaccaag acgaatcata agaattcctg aaatcaatgt ggtaatatta ctatcaaaga
    17341 ttgtccagaa tgcattttca aaaccttgtg ttactgcgat aggaagtgac tttccagcaa
    17401 ttaattcctc tttaattctc tcatagatga tcacattcgc gtcaaccgcc atacctactg
    17461 taagaatgat ccctgcaaaa ccaggtaagg tcaaggtaaa gtccatcaaa gataataatc
    17521 ccattaggat aattaaattt gcaataagag atatgtttgc aactataccc gctaatttat
    17581 aataaaaaat catatagagg ataacgagta taaacccata catcacagat tttaaaccta
    17641 cttcaatcga ttcaataccg agtgttggtc caataaatct catctctaga acagaaagtg
    17701 gaattgggag cgctccctcg ctaataatat tagagagttg gattgcatcc tcctgattga
    17761 aactaccaga aatttctgca tgtccaccag caataggttc attgattcta ggattggaga
    17821 tgacttgatc tccccaaata atagctaaat ttcttccttt gttgttttta gtaacttcta
    17881 aaaatttatc tgtccctgca ggtgtaagtg aaaaagatac agtccatcca taattatttg
    17941 aattaaaaga aggtctagca tctgttagat cgttacccga taaggcaatt gctttttcga
    18001 gcacgataaa tcttcttgga agtaaaggag agtttggctt attccctcta gcataataag
    18061 catagagttt gtatttatct cttggaattt tgtatttttc ttctagcata aataaaaatt
    18121 catcttttac ttttttccca ggacgactcg ctacaacttt ttggaattga acgatctcag
    18181 tttcttctct tttgttttga gacattaact cctgttctgc tttttcaatg agttgagaat
    18241 agctgttggg tccatctggt tcttctaatc tgtattcaac tgtttctgtg ttctgaagaa
    18301 tttctaggat ttgggaagag tttgcaactc ctggaagtga cacttcaatc gaatcctgat
    18361 ctttttgaat acgaacctga ggctctgtca aattttgatt cgttaaacga ttgtcgataa
    18421 tcagctttgc tttttccaat tcgataatct ttcttgattc tgttaaaata aaactatctt
    18481 caatgtcttt aagacgagct gttgctttat ctttttcttc tttcgatttg tcgttagctg
    18541 ctatcgttgc ttttaggtcg gaaatttctt tagaataaat gtctctcaac ttagaaacat
    18601 aattttcaaa atctcctttc attacaactc gcataccacc ctgtaaatct aatcctaatt
    18661 taatagtcat cgatttacca tttttaaaaa atttctcgat aggtgttggt cttaaattat
    18721 ttttagattc aagaaataga ttaatatttt cctgcgaaat ctgattgatt ttggcagatg
    18781 taataaatct ccctgttact aaaaaataat tttgagcagg aataccaagt gctggatccg
    18841 gctcaatttt taaggcgttc tgtgtattgt attcagcctt ccatcgttct tgaaaacgat
    18901 ttaaaagttc ctgtttttcc tgatctgtca tacttgccac agatggacta atatgtaatt
    18961 ctaattccct cactgcaaag tttggaaata aaatcacaag agataaaact aatatagtta
    19021 tgggtagaag tacccaagat gaagatttca actctaactc tcctacgttt ttattgattt
    19081 atttgtttcg gaatttgcta aaaattctgc cactattatt ataatcccgc ggacctacat
    19141 accttagtcg gtagattgca aaaattataa tagcagcaaa tataaatata actttcttat
    19201 attcatttat gaaattaaag atagcatttg acttagtatc aacaatagtt ttctcaggat
    19261 ttttcttctc agctggtttc tcagaaacgt tagatgctgt cgttttagct ggaggcggat
    19321 ttacttccgt tttggctggc tctagtcctg gaatattttc tggattcatc gctaagtctt
    19381 catgaaccca atattctaaa tttagcttat tattttgaat ttgaggtaaa ttcggagtat
    19441 gcgctaccgt gtttccttct ccggtattcg caactggtgg aacgggcttt gttttacgtg
    19501 gatctgtctt agctgctgtt ttcgctggag gaactttttt accgtttttc gttggtttct
    19561 tttttttagc ggcagcagga ttcgtctgtt gtgttacttt ttttgtatct gtctttttag
    19621 ttttatcttc taggacttta tctaaaaagt cggaatcatc gtcctgagca taaacaacaa
    19681 aggtaataga cagactaaga agaaaagtta aaaatatgcg tagaacactt gtcactgcat
    19741 ttgagaaatt gaatagaact tggttcgtct ttgtatgtct caggaaattt ttcatattaa
    19801 gattttttct ttaaaatggc actcgttgaa aatgtaatat ttgtttcttt cgcaacattc
    19861 agcactacag actcattatt atctttgaat tcgactactt tgccatgaat accacttgta
    19921 gtgacaaccg aatctccttt taccaatcct tggatcattt cttgtttttt cttttcttct
    19981 tttctttgtg gctgaatcac tagaaaatac atgatcacta acataattgg aaataaaatc
    20041 agtgcagaga gtgaagaact ttgacctcca gcatcctgtg caagataaat caagtattga
    20101 aacgttgcaa gtaaatacat attagaatcc ttctataaat aaaaacattt gtaatgtcag
    20161 tttgaaaatt gcagaatgtc agtcattaat tttttaaatt ctgaaagaat tccattactt
    20221 tacccgatcc aagctgatca aatgccatgt ggtagccttc tttgatattg ggagcttttt
    20281 ccattaaata aagagcagcc cctgcgttta aagcgacagc atgagatcca gaaagcttct
    20341 ctccagaaag aattctttgg gataagctaa cagcctcttc cttggaatta caaaaaatct
    20401 cattttcttg aatcgatgaa agacccaagg aatctggact aaaaagatga gtggagactt
    20461 cgccattttg ataaagcaaa taatttgtat ccgcaaaaat tgaaaactca tcaaaaccat
    20521 ctacagaatt acatacgata gccgccttag ttccaagctt tgttaggaca tgcatgacct
    20581 taggaagtaa ttcttttgta tatacaccta aaacctgata aggcggagag aatggattac
    20641 tcataggtcc tagaatatta aatactgtcc taaatcctaa ttgctttcta gtctctacag
    20701 caaatttcat agatggatgc caatggggtg caaataagaa agtaaaccct ttttctctca
    20761 aacggctctc tgcttctgaa tgggagacag ttacattata ttctagtgcc gaaagtagat
    20821 cactagagcc acagagagaa gatacggatc tgtttccatg ctttgcaatt ttaactccca
    20881 tagatccaag tgttaaagcg gataacgtcg aaatattgat agattttttt tcatctccgc
    20941 ctgttccaca agtatctaaa taatcaaaat taaatccaga agatggctta acacaagaat
    21001 ctctcatagc tcttacaaat cccactaatt catcagcagt ttctactctc attcgaattg
    21061 atgtcaaaaa agaggagaga agaatatcgg atacttctcc tttcataatt gaaatcatca
    21121 aaaaatatgc ttcttcttct gagagagagt tcccagctac ggatttttgc aaaagagaat
    21181 ttagattcaa ttttttctcc tgataagttt tataatggta aatggattta atagttcata
    21241 attagtaaag atataacgaa gaccagaaaa gacagtaacg atagtagtaa tgagcataac
    21301 ataataagga agaaaagttg ctgctgaatc tacccaatta aaattattca tagtaaactc
    21361 ttgaggacta ttcataaaag ttgaataatt ttcatatgca atttgatatc ctgattttcc
    21421 cgaagcttca ccttgttcat acatagagtt aataacgttt ctttgtttgg tagaaacagc
    21481 cataaacaaa acgagaataa caaagattgc acccatttga aatgcagttt taattttacc
    21541 catcatggta gtatgaagag atgttccaga ttgaattgct aaccatctaa gagaagtaat
    21601 tagcatatct cgtaaaatga ttaatagaac catccatagt tcaatttgtt tatctaataa
    21661 tatgaaagtc ataaaagatc caatgactaa tattttatca gccaaagggt ctaggaattt
    21721 tccaaactca gtctcttgct tccactttcg agctaaatat ccatcaatta agtctgtgaa
    21781 agaggcaaga aggaaaatta cgagagcgta taatctaaaa aaaaaatcgt cttgtattaa
    21841 aaaataaata aaaaatggaa caagtaaaac tctaagaacg gttaaaaaat tcgggagatt
    21901 aaaatctgat ttttccattc taattctccc aagttccatc catatcatat tcggagaaag
    21961 aattgatctt aactcttcca atctgacctg gttcaagtct tgcattttga atatagacag
    22021 tctcatcaat ttcaggtgca tcctgaaatc gtctcgcttc ggcaatccca tcttctacac
    22081 tgtcaacgat agcatgatag gtttttccta ctcttgctaa atgaatttct ttgagtatgt
    22141 ttagatgtgt ttctctaact aaatttactc tacgagctgc ttctttttta cttacatcta
    22201 gctttaactt ggctgcctta gttccttctt gtggagaaaa ggcaaagaga gctaactttt
    22261 ctggtctcac ttcttcgaca aaacgtaaaa cttcatctac atcgtctgca gtctctccag
    22321 gataacccaa aataatagaa gttctaattt caagatcttt cacctgacgt gctttcgtaa
    22381 ataaatctct aaaaaaagaa aaagaaccag ttcgattcat tgattttaaa actctttccg
    22441 aaacatgctg aatcggcgat tctaggtaag gtgcaatctt tggatttgaa ataaaaagat
    22501 ctaaaagctt ttctgttttc ttatccgggt aaagatagag taaccgtaaa agttctaaac
    22561 cttttatttc ggttatctta gttaatacat cttttaattt atctgtgtct cttccataaa
    22621 aaactgtgtc ctgagaaaca atacaaattt cctttgcgcc tctacgaata gcaagtctgc
    22681 tatcatcata aattctatct tcgctttgat ctctgaattc tccacgaagg gaaggaataa
    22741 tacagaaatg acatccacgg ttgcaaccat cagatacctt tacataggca tatggtttga
    22801 aaccctgcat tgtagataaa acatctctat ctataagaga atcattcaca tctttaaaat
    22861 gaataaagtc ttgtggaaat tgctctttaa taattttacc agcttggcta taacgccctg
    22921 ttccaaaaac taaatccact tctggtatat cacttcgaat ttcttttggg tatctttctg
    22981 caaaacaacc aacaacaact agtctttgtt catcttgttt ctttattttt ccagcagaca
    23041 gtatagtgtc tatcgtctct tccgttgcag attgaataaa tgtgcaagta ttaattaaat
    23101 gataatcact ttcttctgga gacttcgcag gaactagacc ttcatctaga agagattttt
    23161 ccatatgcaa agaatctgct gtgttctttg gacatccaag agttgtgatg tagaaagatt
    23221 taagagcaga tttttcgtgc accgaagatt tcaattttac tctccgagtt ctttgataat
    23281 aaattgagtg ttatcgtatg ggtttggaac ttttaagaag attttctttg caagtcttcc
    23341 tggcttacca agttttactc tatcttttcc attttgaatc atctcaacag ctcccccatc
    23401 ccctacttta atttctaggc gatctctagc ttctaattgc tttgtttcac cactggaaat
    23461 aagtcctctc tcgcctgttt gcccatcaat aataaattct acatagctag agcgaataaa
    23521 atatagagtc acttggatag gaacatcgcc tatcggtttg ttattactat ctggtttaga
    23581 atcccccaat agtcttacta aaatctttgc ggaattatcc gttactgctt gggtaacgat
    23641 tttaatttct ctacgaagcg aagttaattc tggattgtta taacttaaaa ccgtttcact
    23701 tcctactttt gaatcaaagg tataaacttt tttctcaggg aaaatattaa aaccaatgga
    23761 agcaaagttt tctccatcaa ttttcttaac acctttgata aaaattttgc attgttgatt
    23821 attgacactg aaagtaaatc cctgctctgg tgttaatgta aaaggaacac ttgaattttc
    23881 aggaacagat tgagaaatat aggatatttc cggaatcaat ggacttgtag gatttggagt
    23941 atcagtcgtt acctctgctg gcttttcgtc gttagttgca gtatctcctg attcagaaaa
    24001 gaagatcaca agaaaaacag caaggcagat tcccaaaata ataccaagag gtaaaaactt
    24061 atttgttttt aactctaggg caatcatttt tactgttggc tttgttaatt cttctaaggg
    24121 ttgttgtgac tcaactaatt gttctcctcg gtagagatta atcataccgg ctgtatccaa
    24181 ctttaaataa tcagaataat tttttagaaa accaaccgta aaggtctcag ctggaaattg
    24241 tgcataatct tcattttcca atgctataat aaatttaatt gctatgttag tatcacgaga
    24301 aacgtctttt acagagagtt ttttttcttc tctgacctct ctcaaaattt gtccaactct
    24361 tttgttagta ttcactgaaa tctccaatca gatattttta ttgattatcg cttactaaag
    24421 gattatttac gattttagaa tttccactaa tatgataatt gaaagttcca tcctctactt
    24481 ctggatctag aaccatgttt gcaaattcaa tagtggtttg tttgcctctg gaatcagtgg
    24541 caatggtttt tttaattaaa taggtttctg catctacgaa taatagcata ttctcaaaac
    24601 caccgatctt ttctctttgt tctaaagata atacaaagta agattttcca tcgttatttg
    24661 cattgcgggg ttgtgagatt gtatcaaatt tataatggta ttttcgaaag agtcgattga
    24721 gtccagcagg agaagcagat tggaaaatag gctcaccaga agtattctgt ttattgattt
    24781 ctaagtcttg ttttcctacc gcattgattt ttttaatgta aatccaaaga gtcttcccat
    24841 cggatacgat taaatctcca tctggttcag caaattcgta gcgaatctta ccgggagctt
    24901 tgtaggttgt cttcccacgc atattggtcg tttttttatt attcacagtt tgaattttaa
    24961 attcagccgt gtaggatttt aaatccgcaa acttcttttt gatattcttc acaacttccg
    25021 aaggagaatt ccagttatga gcctgcgcaa aaatagaatt gctaaacaaa aaaaggaaaa
    25081 tagagattag aaacagaaat ctttttataa aaagagtctg attttttaga acttgctcat
    25141 tctttgtagt tttgtgaatc aaaaagtgca tagattacct atcaataaaa caatacttta
    25201 aaacctaagt aaattgttaa gacaaatttt taatgattcc agtgagtcat tgctttataa
    25261 agcctttaaa atccacagat aaacaggcga aggtgcaggt cgcgagtatt tggagtgatg
    25321 ggtagaatga acatagatga gtatggtaat ttttttttag cagcctcgct catttgcatc
    25381 taaattcttc tctcatcaat tcccatttgt ttcatgagtg aatgattata atttttagaa
    25441 tgagatttgt tgaaattaaa aagtatgatt tacactgcct agactgaaac tgcaaaattg
    25501 taaaattaag ataagccaat agataaagaa taaaaaagca aaaacggtca agtagccaac
    25561 tccaaaatag aagacaatat ttgtaaagga gtgaataaaa tgaaactaca tacagggatc
    25621 attacaaaac aaattcaaga atccaaaaaa ttctatacag aggttttaga ttttgaaatc
    25681 aaattcgaat ctgaatggtt tatactttta aatgtaaaaa ataaaccaaa caatgaatta
    25741 gctctaatgc taccaaacca aacccaaact agaaaaacct atttccaaga agaataccaa
    25801 aaaggaattt ggtttatctt agaagtagag agcataaaaa actattatga aaaaataaaa
    25861 actaaaaaag taaaaattga tttagagtta acggaggaag aatggggaga tattcatttc
    25921 acgattcttg atccgaatgg aattggtata gatatagtag aagaaagaaa atcataaaga
    25981 ccgatttaga aggcagaatc tatttaggta agaaaatcat taaagattta aatcatattg
    26041 gtatttttat cttggatcaa ttaaaacaaa aaggttctgt cttctttatc ttgaaatctg
    26101 taaaaagaat caaaaagtaa gctactctac tcatactatt tgtattcttt tgaagaagcg
    26161 attgaaaata ttcactggac ttaatttacg atacaagaaa aaaagagact cattgattct
    26221 atgtttcagt cttatgaaaa aaagtatagt tcgaaaattt ttccagtgca tgaatacatt
    26281 ttagaaaatc tagatagaaa aatttcttta aatcaattgg cttttctttc tggattttcc
    26341 aatttccatt ttcacagaat atttaaagaa cttcagggtg aaccaattgc tactcaccta
    26401 aaaagattga gattagaaaa agcggcgttt gaattaaaga tatcagaaat tccgttaaaa
    26461 gaaatttcga ttcaagctgg atttgattct tatgaagcat ttagtagagc attcaaaaaa
    26521 ttctttttat taagcccaca agagtttaga aaaagaaatg caatctctca aaaacaaatt
    26581 catcacttta gtaatttgcc aaaaggaatt aaactcaacg aaattcatat tcgtaaaatt
    26641 gattctgtaa aacttgcctt tctaagaaaa ttaggacctt acgaagattt acccggacca
    26701 gatttaaatt caaaagaatt caaaatgcta cttgaatttg taaaactaaa ccaaatgaat
    26761 ccaaaaaatc atttgatgat tggagtttgt caagacgatc cagagatcac taaatccaat
    26821 tttgttcgtt ttgatattgg ttttagtatt acaaaaaaat gcaaattact gccagaaatg
    26881 ggaattcaaa ctttaaaagg cggaaattat ctagtgtgta gatatagagg agaatacaaa
    26941 ggcttaactg aaatatataa ttttctatta tatgaatatt gtccaaagta taaatataaa
    27001 ataacaagtg atcctctttt tgaagtttat atttatggat ttgaaaaaga aatagctgat
    27061 atttttatac cactaaaaaa atgattctcc tgattgagta gatgcaatta aatattctgg
    27121 aatttcaatt tttatcctcc actccactct taacattctg ttgaaattat tatcacatat
    27181 aatttctctt atcaaaaata ctataccatt cctcacgata caatcactca gagtctagcc
    27241 taagcaaatc taaaaatcat agtcaatctt taactctgaa tcattgagag taggttttct
    27301 ccaaagtatt tcaaagttgt ttagataaac ttttttccat ttctaaatgt ggatgcgtta
    27361 aaagaaaaag aatgcttact caaagaaaac aaatccattc aatcattcga tattcgtaaa
    27421 tataagtgat aagaactcgt gccacaagat caaaacgatg attgtactgg aatggataaa
    27481 ctgagaaaaa taacgattgc tttttacgat aattaataat aaatctaaaa ttgacaatcg
    27541 ggagaaattt tatgccagta gcattagaat tagactttaa acagttaaaa acattaatcg
    27601 atcaattaaa catagaagag aagaatgaac tttctaaata cctaaataat cttaccctat
    27661 caactagaat taaaaagcta cgtgcctcta ataagaaagt aaaaatatct gaacaagaaa
    27721 ttatgagtgt ggtgaaagaa gtaaaaagaa aaaaagcaaa atgaaaattt tcattgatac
    27781 aaatatttgg attagttatt tcctatctag atcctttggg aatctagatt caattttcgc
    27841 taagaaagat attgagatat ttacttgtat tgagcaaatg gatgaactgg aaagagtatt
    27901 aagatacccc aaactaaaga attatttccc tgaagatgta atcgcagatt tattactttt
    27961 tttttcagaa aaatgcacat tagttgccat tcaaagaatt gtgcaagagt gtaatgatcc
    28021 tgaagataat tatattttag atattggtat acaacacaat ctaaactata tcataaccgg
    28081 tgattcggat ttactctcca tgcatccttt ccataaactt tctattctaa agattcaaaa
    28141 ttttttacat ttaatagaat gagtatattt cattttaaat ccatttaact cgtagtcgaa
    28201 caaaaagaaa tagttcaaaa actgttagag attgtagatg cgatagagga aacatacagg
    28261 gtaaatttat agcaggtgct agaacagttt caagctgaaa gggagacgta gacatgaaaa
    28321 accaaagagt atcttatagc tggtatgcag taaaagaaat tgcaatgtat gtttttaaaa
    28381 attacgaatt tgaaatattc tggataaaaa aaacttgagg atatttagaa gaaaatttaa
    28441 aagcaaccaa aaacggaaaa atgtttgaag ctctcgacaa tgattcatca gaaattccaa
    28501 aggaagcttt tctttggatt cttgctttat atagtctcta ttttgaattt aaaatcagca
    28561 tagattatga aagctatgat acactgcctc caatggatga tttttttgaa tcagaagaaa
    28621 tttaccaaga ggaattaaac cggattgatt acaaagaaaa acttacggga gatattgaga
    28681 aatttcagaa aatattcaga aagcttttgg taaaaaccta tcctgatgat ttggataaac
    28741 tctcattttt tttaaactta gatgtctgtt ggtcagaaga agaatttata caattttata
    28801 cagagatgaa tgaatctgaa atcacaaata ccaacgattt ttgtttggtt cgagatgaaa
    28861 aatacaataa atatctaaat actgtatcca tccaaaattt agaagagagt aaaatgatgg
    28921 atggacttgc ttttgttgga gagttatgaa atttagattg tgattgtggt agatcttact
    28981 cgacaaagaa ccactaaatc atattcccat ttcctttcta gcgactaacc tccaactttc
    29041 tcactaatct tccctattcc atcttaaaag ttgatgacga aagagcaaag atagattgcg
    29101 aatctttcag acattgatgt ttataactaa acagggaatt tttttctata aaacaaaaac
    29161 atcactagca taataccatg gaattaacaa gtattatccg ctaaaataaa aagaaaatca
    29221 aaaagaatta aaatgaattg cgaagaaaaa aataattttt atattgaaaa caataatata
    29281 caaaggattt ttcaaaaatg gaagaaatta aatttgagaa tagtatggca caagtaaaat
    29341 ataataaaga tttaaaaatt gttgaagtgc attggaaaaa atcgaccgta gatgaattta
    29401 aatttcaaga agttttaaac aatggattaa aagaactatt agaaaacaca acaaataaat
    29461 ggttagcgaa tacgagtcta ctcggtgtta tcccccagag aactgctgaa tgggtagaaa
    29521 aagaatggtt ccccaatgtt ttaaaaactg taaagaaaat taaaatggca attatagtcc
    29581 cagaaagtat gattacaaaa atgagtatag ataaaacaat caagacagat gttttagagc
    29641 agcaatattt tgaaaattta gacgaagcaa ggaattggct gaaaggataa aattaaggag
    29701 attaaaaata gactaaaatt ttttttgtgt atattgttca aatatactaa taattttata
    29761 actcttgctc ctatttacaa aatagaggca agagatttaa caataatagg ttaaagtata
    29821 agacaattat tacaagtttt tatccaataa ttcggtggag taattcaata aaaaagaacg
    29881 gataagggaa tagaataatc aacaaaccta tcagaattca taaaaaagca aaaggaggat
    29941 actaagtcat tggcaataga gccaatcagc caagtcatat aaatttttct ttggaaggta
    30001 acctctgatt ttaactcatt cgaccacaat ccaaaataaa ttctaataca agtaaaagat
    30061 aaatttttta aatctagcgg atatcgctaa ttttcattca attctttcaa aattgtctcc
    30121 tattttttcc ataacctaaa agcttttcta cctcatctat tttctgtatc aactctccac
    30181 atatcgctta tcggtgttca tccgtggtta atcttttaaa aatcccctac tccggtaaaa
    30241 acagcttctt aatctcagga aaatttttct ccgcttcaga ttcgccttta tcaataatgt
    30301 ctttataaag gctaaaatca aataagttaa aatcctctaa atgaaagtgc aaaaataaat
    30361 ccatacgaga cactttcgct tttgtaatct cacgaccctc taacatactt gctctaccca
    30421 tgatttttaa gattggcgga tacttgatat attcccataa acttctaagt aaagatttat
    30481 tcgaaatgga tttatcatcg aagagttgat aaaaagtttt atcttttaaa ggagaaacat
    30541 tcacacttaa aattttatct gctccttttc ttcgaatcaa atcatctggc acattgttta
    30601 aaactcctcc atcaatgaga actctttccc caatgctaac aggtgggaat acaaccggta
    30661 gactcattgt gcaaagaaga gcctccaaaa gggtcctttg tcaaatacat actcttcccc
    30721 tgcatgtaaa tcgatggccg acgttacaaa ggggatctta gaatcttcta atctctcatc
    30781 tccaaatatt tctttgagca ttgctttcat tttacgacct ttgtaaaagg aaatgagtgg
    30841 aatggttgga tcaaagggag tatctaaacc accaaaatat ttttttacaa aattctctat
    30901 attttctgcg ctctcccctc ttgcatagag tgctccaatg actgctccca tggaagagcc
    30961 agatacataa tcaaattgaa ttttatttcg ttctaaaact tttagaagtc ctacatgcgc
    31021 aagtgctcta gctcctccgc cgcctaacgt aagcccacga gtcctagcca taatagaacg
    31081 agccataaat tctcgatttg tatagacttt ttctttttct acatccgctc tataagatat
    31141 tgatttttcc tgaaaataac gaattgctct cccctcataa ttgcgcagtg agtttcttag
    31201 atattgaaat gtatttattt tctcagtcca acctgattcg atggattgct cataaaataa
    31261 aatataatcc gcttgaatta gtatgttttc aaattcatag atcttatcag gagtcttaaa
    31321 taaaacatgt atcacaggag atttagatct gagatcactc aacttgataa tcattttctc
    31381 ccgagaaagt ccaataaatt cctcagtgga gatcattcgt gttttcccga caatcacctc
    31441 ttcacataca ctcgcaacct ttctaacttc ttctgaaaat ttttcgccga tccccatttg
    31501 cacatgagct atgagtcttc taggcataga tgaagatttc gatacaatta ggttatcacg
    31561 aaatcgctca gagacaaagg aaatcaaatt tctagaaagg tttcgatctg tttcagctaa
    31621 atctaaaaat aaatctcctt ttaattcata taaaagacaa tccatagagg cggaagctgt
    31681 gcttgagtgt tgcatttttg taatcacact cacttctccc aaaatttctt gcggtcctag
    31741 gtaaatggat ttttgattat tcacccgaac taaaacttca ccatagcgaa tgataaaaag
    31801 actttctgag atctcccctt ggaaataaat aatgtcttta ttgtgaaatt ttttttcaga
    31861 gaggctgagt gcaatcttac gcaatgtctt agattgcacg ccttcaaaaa gttttaattt
    31921 ttttaaaaaa cgaattcgaa actcaatagg actcataggc atatttatta tttatatcga
    31981 attatacaga actcaataaa taatttttct tttagtaaat ttatttcatg caaataaaaa
    32041 acgagcctaa ggactcctga gaaattatcg tatctcttac aagcggatgc tatttctttc
    32101 cgaaaatgct ctaaaccaat tcaaatcgaa ttctattatc agtgggaatc ttttcttttt
    32161 tccctcttat tcgcacttac tatttcgata cagtattcga aatactgttg atctcactct
    32221 agaaatgaat tacatttttt taagttttct ttttttttaa ttgattgttt cttttcttct
    32281 attaattcat gcattagtga atattgaaaa tttaacatat aaattaaagt caagtgaacc
    32341 gataacaata gagcttcatt cgctagatcc agatactcag aaatttatct attctacact
    32401 cctagaaatt ttaaattttt acaacaaaga ggatttattt actgccatct ataccgcttt
    32461 atttgaatct acgatgaact gcgtgaaagc aaatgcaaag aaagcttttt tccaagaaaa
    32521 agatctaaaa gtttacgatg aatttgattt tagaagcgga accaaactat tcaaaaaaca
    32581 ggtgaatgat aataatcttc cctattattg tagccttgcc aaaaaacttg gacttacaat
    32641 tacaattaac tttatacatt cagtcaaagg cttaaaagta gaaatcttaa ataacggaca
    32701 tcttttcaaa gaagaagaaa atcgaattcg aaccaaactg gctctcggtc aaaaatacgt
    32761 taacattgta gaataccaca aagataattc cgataaaacc gaaggagaag gtcttggact
    32821 agtcgttagt atcctaatgc taaaagaagc tgggattcca atcagtgact ttagaattgg
    32881 ttgcaaaaat ggcgttacac gaactagaat cgaagtccca tttacttcca gttatatcag
    32941 cgaaagacaa aaatataaaa tggagtttga tagacaaagc ttctaagcat tcgggaattc
    33001 ttctttccca tctgcatgtt ttgcaaaccg tcccttcttt cgatcgtgca caggctctga
    33061 actagtccaa gaccaacctc caaaatgagt tgcttgaaaa tcattaaaag catcttggat
    33121 ttcttgtctt gtattcatca caaacggtcc atattgaaca actggttctc caattggttt
    33181 tgcttgcaag agaagtagtt gtgcttcccc ttcaccgttt tccaacaata catcttgatc
    33241 tgaaattaaa tcaatattat tatacggcat aacagtatta gccgcaacgg ataaactttt
    33301 cccacggaaa aaataaagca ttcgattcac cgaggaatta gaagcgggta atgtccattt
    33361 ggctccactt tctaatcgaa tgatccaaat cgcaacttgg tttttaggat cagctgccca
    33421 agaatcaggc ggaggactcg gagctttcat tccaccgagt gagcctgcaa tgatttccac
    33481 ttcgatcttt tttccatctg tatcttttgc tgtgtatcta ggaatcgatt cattccaaag
    33541 catggtaaaa tgtggtttta caaacttatt cacacgaggg agatttaacc aaatctgaaa
    33601 gagttctagt ttattttctt cgtcttcttt tatcagtgga aacatttcag aatgttgaac
    33661 tccagcaccg gctgtcatcc attgcacatc gcctgccgaa taacgtccag aggcacccat
    33721 agaatcagaa tgatctacaa atccagatct tacaacagta actgtttcaa atcctctatg
    33781 cgggtgattg ggaaaacctg gaattttttc tccatgatac attctaaatc catcttttac
    33841 ctcaaaatcc attccaatat ttctgccttg caagtattga ttcgcaggtc ccatagaaga
    33901 atttcctttc ggatagtaat cttcatgatg cacacaaaat aaaaatggat ccctcgtctc
    33961 ccattgaaat cccatttttg atataccttt gatcggattt tgagccatgt tccaattctc
    34021 cttaccaaat aattttgaaa gattaaaata ctttattaaa cctgtcggaa ttcctatcgc
    34081 taaaattcca agaactgctt tttttagaaa tgattttcta gaatcagtct gttgcgaaat
    34141 gttaaagaat taaaagtcag cctttaaaaa taaatg
//
LOCUS       DAOQOS010000053        33773 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_553_length_33773_cov_6.119995, whole genome shotgun sequence.
ACCESSION   DAOQOS010000053 DAOQOS010000000
VERSION     DAOQOS010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 33773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33773
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_553_length_33773_cov_6.119995"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(133..1386)
                     /locus_tag="PLF66_13020"
     CDS             complement(133..1386)
                     /locus_tag="PLF66_13020"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HMZ65013.1"
                     /translation="MHKQSFKILAFSLFTISIFILALHGFFLALCKINKIPNFRFDSF
                     LDPNFRLDNPNFEKELTEGDILIYLPQSKLRKSTPDGLSLKFAGETVTYYSGYRRTSP
                     IQREKGKGVKRVISVGDSFTWGWKVSNEDTVSNHLEQLLNQSKPNGVEKVEVLNAGMT
                     ASTITNSYESLKNYDVQFSPDVVLLTFCENDISDLKNRNATDAFTYDMLKKIGWFGPI
                     RFFIAGKYKKERELLLKQIKEMNQNDLVFEKFGIKPSISHIDWPNDLKKFTQTKIDRD
                     DIKNLVIHYYLKNEGNILPYTKEDDKETWELWKEWEEALVKMKKLSEDKNFKLIVYTY
                     PIFVRIHEPQFDNRDTAENIIEAICKRHNIMHINPLKLFREEDKKLRSEKKYLFFLPE
                     DGHPTAEGNKLSAKVLAENIKPLLE"
     gene            complement(1643..2398)
                     /locus_tag="PLF66_13025"
     CDS             complement(1643..2398)
                     /locus_tag="PLF66_13025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65014.1"
                     /translation="MDLLNMGIPGTGPREYFSQFMGEGLELKPDMLLLSFYIGNDFNE
                     SFRDYLDENGNLIDPKKEKWFKKSYLFTFFHYFLTIQTKFEGNIFNGLTEYCDECPNF
                     EEERFLEIVSFNRENFSPNNVLLPAMIDKAFKYIEQINEICRKQNIKFRVVIIPEEVQ
                     VSSKFGTFLFNKYQKYYKDRNWDMELPNKIFRSKLDSLGIKYLDLYPIMKNHPKSDFL
                     YKPRDTHWNILGNQIASDEIKKFIQEDIPLNRK"
     gene            3008..4834
                     /locus_tag="PLF66_13030"
     CDS             3008..4834
                     /locus_tag="PLF66_13030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002973828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase"
                     /protein_id="HMZ65015.1"
                     /translation="MNILGISCFYHDSAAAILKNGEVVAAAQEERFTRKRHDANFPNR
                     AIEYCLETANLKVSDLDYVAFYDKPFLKFERIIESALSAAPKGLPTFMKAIPIWMSQK
                     LFAGEEIKKQLGKKEFKGKLIFPEHHESHAAAAFFPSPYLDAAFLTVDGVGEWTTTSF
                     GVGRDNRIEILSDIKFPHSLGLLYSAITYYTGFKVNSGEYKVMGLAPYGEPKYVKLIL
                     DNLIDLKEDGSYKINLEYFDYVGGDRMTNKKFDELFGAPARKPETSLTQREMDLARSI
                     QEVVEIVVMKMARHIHKVTGMKYLCLSGGVALNCVANGKILREGPFDDIWIQPASGDA
                     GSALGAALITHYHINNAPRKGTNFARLQKASYLGPEFSEEYIENYFKTNNIPYEKVPK
                     TEVAKRTAKVLSEEKVVGWFQGRMEFGPRALGARSIIGDPRSRNMQSIMNLKIKFRES
                     FRPFAPSVLREDVSKYFEHEGDSPYMLIVAPVKDSIRIKMTPEQEKLFGIEKLNVPRS
                     TLPSITHVDYSARIQTVSREDHPMYYDLISEFKKLTGVSVVVNTSFNVRGEPIVCTPE
                     DAYRCFMRTNMDYLVVENFILDKTKQPELKNDSDWKNEFELD"
     gene            4821..5234
                     /locus_tag="PLF66_13035"
     CDS             4821..5234
                     /locus_tag="PLF66_13035"
                     /inference="COORDINATES: protein motif:HMM:NF039992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SxtJ family membrane protein"
                     /protein_id="HMZ65016.1"
                     /translation="MNSTNKEETKAKELKSFSLGGGLLLSALAGFFYYKEKIEIAYVF
                     AALGLAFLFIRLINFKWITPVHTAWMKFAHILGVVNTRIILFIVFILTVVPISLLLKI
                     FRKDILDKNLQKEAVTYWNDRPKKELNIKHYERHF"
     gene            5283..5399
                     /locus_tag="PLF66_13040"
     CDS             5283..5399
                     /locus_tag="PLF66_13040"
                     /inference="COORDINATES: protein motif:HMM:NF039953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5989 family protein"
                     /protein_id="HMZ65017.1"
                     /translation="MERKKFWLLPIIVLLVLLSILLVMTQGSAISPFIYTLF"
     gene            5396..5794
                     /locus_tag="PLF66_13045"
     CDS             5396..5794
                     /locus_tag="PLF66_13045"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HMZ65018.1"
                     /translation="MINVYCDGASRGNPGDASIGVIAYTDSTKEKVLFTISKAIGKET
                     NNVAEWTALLFALEKCLELGEKEISVYLDSELVVKQMNGVYKTKHPDLIDIKKKVDKH
                     KLEFSKIEFIHIPREKNKEADKLANRALDE"
     gene            5967..7610
                     /locus_tag="PLF66_13050"
     CDS             5967..7610
                     /locus_tag="PLF66_13050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65019.1"
                     /translation="MKYRKGYLQNFYWIAHSVPICIILFLFNLSLLSDSFELKDGKIL
                     SGTEVSRTKKNITIKEANGKLKTIPLDKLKFITIEKKIEIKNKETSEEQKDISELRKK
                     IEELNTKIESLSEKQKKEQNNNYKEEVISNKDIKEPSTEKTYTSTGGAKSIHSYSEPP
                     VSDEPNIELTHEIVSDFIWRGNSYGGEYLSRRNNTAYSGTSQYWAYQPNIRLNSPLKG
                     LYIEFWGNFALVGRNDRDSDMRLMQASSGSPAIDPNVYFSKLSAIQTDPSNTNILNSG
                     LFYDPPNNIVNSNCSSDGVGGCSNPNYSFVDPRSVGKHKEKNGMARTDGGFTTFAYNF
                     QNKKYGDITWGVWFYYQMDKNSKYSWDEYFIFWGLPFWEGILKPTVSFYTQSSFESNS
                     LNAGGHYLSFSVSHTFFEGKFFRIQPASNIGYKYPNDNINQKSGFYDVTSNLKFFFAD
                     FFFSLNHVYRPNLYMYDNDVMYYTLSQGSQAQPNFSQYDGKTVDPSKLYGVKNEAVYS
                     AIDKIDSPDIVKSYIKSEYQSQKIIQNLFYISFGFNHKF"
     gene            complement(7607..8698)
                     /locus_tag="PLF66_13055"
     CDS             complement(7607..8698)
                     /locus_tag="PLF66_13055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HMZ65020.1"
                     /translation="MNAVQDTYLYRILIKIVSYTTGIMFHSVHKYFRTKEQLVNAPHP
                     TILMANHVTELDIVALSHVYPHIPERIKFCFAMRQDIIEPDFLIKEFEPKGFIRFILM
                     LIDKSQIIKILLVYVGGIPVKRAFRDDARKLLKQGELRDIVDSQWDQLAEGVQKGRNL
                     FLFPEGKFSETGNLDPIKRGTYFIFKRIPNVSYNYFNFTYDFISENKPVLHIGFGENS
                     HFPQDADEFKVADEIKARLGNNYVITAGNLFSYFILQADVANGISKDILLKRISNFLN
                     KLKETNEYYIAENLLTSELSSLFNKFIEKASSKGFIKIQNETIQSTEKLKTITFRNGK
                     DMRKKNIYLYHKNQLKYYFGSFEKILSEI"
     gene            complement(8748..9149)
                     /locus_tag="PLF66_13060"
     CDS             complement(8748..9149)
                     /locus_tag="PLF66_13060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65021.1"
                     /translation="MNLELKDELELNTKYKFGNEKEISEIIKLFQFSNLNLIISNPEG
                     EIFFASNSFYLFSHYTVKEAIRENIETILGKSFDMFELEILKRELECFSVRRTLKTKW
                     GKKIEVNTYSSILHEFQNIFIVTIIIPAPVM"
     gene            9421..9579
                     /locus_tag="PLF66_13065"
     CDS             9421..9579
                     /locus_tag="PLF66_13065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65022.1"
                     /translation="MTEKEAKNIDPKFTEKSANEFARSIETGELYEELKGKKKPSEED
                     DNSKKNKS"
     gene            complement(9881..10135)
                     /locus_tag="PLF66_13070"
     CDS             complement(9881..10135)
                     /locus_tag="PLF66_13070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003003412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET transporter"
                     /protein_id="HMZ65023.1"
                     /translation="MEFESIIGYIAASLTTISFLPQMIRVVMTKKTEDISRNMYILLN
                     IGVSFWLFYGFLKTDYPIIIANTITLVFSLTILIYKIIHK"
     gene            complement(10150..11466)
                     /gene="xrtN"
                     /locus_tag="PLF66_13075"
     CDS             complement(10150..11466)
                     /gene="xrtN"
                     /locus_tag="PLF66_13075"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR04476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase N"
                     /protein_id="HMZ65024.1"
                     /translation="MLKFAFLPFLLFIFIFLNPYLIRSILYNSNSPISISSFYPLLFI
                     PFFLKNDSKRTLNHLQFIAGLILLIFSIYFFSFNFYWISGIILCVSFLPLLGMNFSNF
                     GFILFLISPPFFEKYDLLISFELRLFLSNLSGFILSFIDRATSVSGNQIFFRNVNYIV
                     DPACEGLKFLIATVLLATVFSYFYFRNGISLPKIIFLSIYWVISIILWIWTNLTRILI
                     LILFGVNSNSIWHSIIGILCFLSLIILPFFLSWIFYIREEVRFYFPTNIKKQYLLIYF
                     LPILLFFLLIYRAKNLSIPDFSWKNNYGSFHIDISEDKESKFYRNQSATFILKKNLPF
                     LGLGHHPKICFEAVGYHFKSEKEIQIDSEIFFRQAEVERDGRKYFLSWWYVEEIENDS
                     RFRSGSEWLWRKKLLTSQNSFLQVNLITNTEKESIEFYKELVKKGL"
     gene            complement(11459..14077)
                     /locus_tag="PLF66_13080"
     CDS             complement(11459..14077)
                     /locus_tag="PLF66_13080"
                     /inference="COORDINATES: protein motif:HMM:TIGR04477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XrtN system VIT domain-containing protein"
                     /protein_id="HMZ65025.1"
                     /translation="MWDLIKNYFTKEVKIFKYENPRYVWILYWIIALVTFSFISIVTF
                     KHLESTHFRGLQPFALFIGGIFSLFLFYLIIRILLEKKRTGFILITLFLSGTAFVVEK
                     YFLNNEIFSPFTPRTTITLWITFFTLFASIFYKNIPKWILLFFPPILIIGAILSFCFV
                     LIMIPAMMIGWVLIIFGIGILPYTPLMAFIGFLYMLYCIVVELKDNQYYLQYTFSKYL
                     TYLVLILTSIYFIWFQSEWNKGQFIIRNHFLETKMINSKRSLIDDDLPAWASLGMKLP
                     VNHISELYLQPESNNREVLFNWFGSDKLFDPFAFVVSNISKKSDLLNEDREHLLRLLF
                     GYTHVSLNRLWNGNSLLTSDIESKIRLYPKARLAYTEIKMSVYNQSGLGQQEAIYTFK
                     VPEGSICTNLSLWIEGKEEPARLTFLSKAQKAYNTIVGIEKRDPSYVTWMEDNLLRVK
                     IFPVLANNYRTFKIGFISPLKTQTNSLEYNGFQIEGPILDSANHKLEANLFEAKDSNL
                     ISDGYTIQKKLSKESDSSYTSWFGYGKYKDKWSIQVQALPSATGRFQFSDLELKVTDL
                     QIQKVDFLPTKIYILFNSSLSKKEWKKIYSDLTSLNLEAQIVLVTNEWFYSRNKEENL
                     DFIENQNLPKFNLFPFYKIANQTDILVITNREDSSIPLEELKGSLFYNKSKEFFGKNK
                     RKLFVYSINQSLSSYLNSLKEYGLIENIRIPYDSLKEALKTKKLNIPENTDNKYSFPD
                     SSITIEMEKRKEKTNVSGNDLLARIVLYKRILYTYGKRFGNPKKDYLEKDLFEMAELG
                     RIVSPVTSLIVLESESDYKRFGIDKNKKDLSKVNLTQDASKIQHGNVPEPEEWLIAII
                     CILCFYFIGKYKFKRA"
     gene            complement(14084..19396)
                     /locus_tag="PLF66_13085"
     CDS             complement(14084..19396)
                     /locus_tag="PLF66_13085"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HMZ65026.1"
                     /translation="MLSFPDFNIKEKIFENQHTIIYKGVYTKDQSSVIIKTHKSELPT
                     NREIAAYRKEFEITSRLQIEGALKSFQLLKDGARYAIVFEDINGISLKSFLVQERIDL
                     GRLIKIFLSIAEALNHIHKNGIIHKDINPLNILIEPSSFQLKIIDFGISSIYSKENIE
                     NMQIDYLEGSIPYISPEQTGRMNRPIDYRTDFYSLGVTFYELLTGNLPFESNDSMDVI
                     HSHIAKSPVSPWLKATQNFPGNDFLLKKLSELILKLLSKDPENRYQSASGICHDLSKL
                     LDFHLKAEQNSPFYLEIGKNDFSGKLQIPLKLFGRDKEKQFLLDAFRRISNGPSEMLF
                     IHGQPGVGKTALVNDMKLTVLNSNGFFIYGKYEEANLNIPYSAFITASREFLRQILLG
                     SKAYIETWRERILNILGVNAKLLTNAMPELEIITGKLKDLDDLDENETNNRFHLTLRD
                     FIHIFQYSRQPILIFLDNIDYADSDSFHLLEYFINNIHSKNILLICSYLNKEESQNIF
                     LSSIKEKYSDVPDLELLNLKTEDVIDLVAETINYPKEDCKELANVIFFKTNGNPFFVH
                     EFLKFIYLEKYLQPIEKSEGGFVWMWKIDIIRNLNINDDMADIVTANLKLLHSTTEEL
                     IQIASCLGNSFTSEMLSMITGYNRFQIEESLIGAVASGLILPSGNSYKFLHEKVQIAA
                     YSLLENKKKLQLHSQIASKLLTTLSEEEIKNDLFSIINHLNLGKNFISPDTSRIRFIE
                     LNLEAGRKAKASAAYDLALNFFLEGIYFFESSAEKKKKELKVNLYLEAAEVAYLLNRS
                     VELETFLQKTKLEQDEDILIKARANEIRNKYLITSGDPIAAINNTIKILKEIKINFPS
                     SPKIYHVLLSILKVKFVLRNKTIYSLRKLNEATDPRKIAAMRLISNIGSAAYFSSPHL
                     LPLLILENVYLSLTSGYTQHTPFSFSAYGIILCGTLGQIDQGYQFGMLALNIYEEIGG
                     IKNKTKILHMVNSFIVHWKDHIRLTLDPLWECYKSGHETGDLEFAFYGLIQYSIHALF
                     AGKELAPLEKEVATGLKSFESYKHQNSSYQMEVIRLTMVTLMEDKEDSFSTLDKAEWI
                     LRDNKLDPNTIEANKFYYHFFNLYINFIFGRYKESIQEAKLATSYSGSMRSLAVYTFL
                     VFLESIALLQEYDKGNILQKRNYLSKVAKNQRKLKKWSKFSKANFLHRYHLIEAERNR
                     VQGQILSAMDNYALASQLSKESEYINDAAVISELTGKFYLELDKLDIAKTYLNSALYY
                     YHIWGADRKVKQLEEKYPGIVDNIYKKDLNSETESTSIITHRGDSESFNLASMVKVSE
                     TISREILLDKLLESLVKILIENAGAERGVLILQKDDKLFIEAEASFDKNNISVLQSVP
                     VENADLPLSIINYSVRFNEYIVLNDAAKSERFSKDPYVLKNNSKSILCFPIFHKQGLF
                     GIFYLENNLTSYAFKKNSVELLVTISTQSAISIENARLYSDLENRVNERTKELDESLK
                     QQYLLNNNLMQITEELNLSLRKIKKDLALAKKVQDSILPKNLERIHSLNISTFYSPMD
                     EVGGDFFDITEINPYKIRIFLADATGHGTQGALITMAIKSEYETLKISPLNTAEIMEK
                     LNNEFLRKFLSVNAFFTCILLDIDPLRGKLVYTAAGHPPQILIRNEQIQKLSSSGKMI
                     GVKENVKYRETELDFESGNKLFLYSDGIFEEFNFMRNEFGEEKLWEILQSHAKSSVSE
                     TLNSVVQQVDDFIGNLPQEDDMTFIGIEYKENFKIESRKDRNNL"
     gene            complement(19400..19996)
                     /locus_tag="PLF66_13090"
     CDS             complement(19400..19996)
                     /locus_tag="PLF66_13090"
                     /inference="COORDINATES: protein motif:HMM:NF018701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1318 domain-containing protein"
                     /protein_id="HMZ65027.1"
                     /translation="MNRNTIFIFFLSFVNCILTPPPITFTQSQTAAEKQMVGEDKELE
                     KDGWLISSIKTSSSGSDEWKKDSILYEESEFKDKDYSSLLGVIAYLAPEVKTYRTKGV
                     LGEGLNGLLQKPAVTLDKPTQDEYSNPEKKKRVENVMKLINDARVRIYETRMLSLSKT
                     ERSKEEQSKLREKILLSNYYTLEAGEYYESSKGNWIKK"
     gene            complement(19989..23033)
                     /locus_tag="PLF66_13095"
     CDS             complement(19989..23033)
                     /locus_tag="PLF66_13095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65028.1"
                     /translation="MSSVNLQTLKSFLKKKSFLFFLFFLILYKSIFNVFSGKYLFTYI
                     FKNYSTGSFEGEVKQFSLFYGIQIDSIVIRSGENFNKEILFSCDRVAFLYNLPYLFLG
                     KLKLSEISLTKPFIQILERNNKLNIAELFVSNATKEEKPKESKPLPEEINLYLPVSAY
                     LNFFIQDLSFRMEKEKGDNFFLTEEKDIDLKILIATKRFRKIPLDASIVNIFETIDIS
                     LNPKRDLQIFYQDNHNQIKQGLSTSLAIYLDSNQNPTAFHSNLKFGSKQIELTVKNRK
                     LPSFAFEASYDLNYLPIDDSLVLENLNIHFDQDDWIRAKGKINKVMTQEREFAIDLYE
                     SKLDLSPVNSIYRQIPGLPDLDFKGKLELAPINVNGTMNSLTIKADIKGKDLLLKQKS
                     KSHSIPYLNLNALANLDLSTKKEPTKENPIPILNSVELKTDLSYNTMLANLKAQLENE
                     KNISAQLNLKNLFLRNFTNAVSGNLSGNINIEGTNFSNLNTKLDSQVRSLEYSVSGSK
                     SGINHVDLSLLGKVALGEKFSFQSLEVNNLIAKIKNPEMSESLSLGANGKLSMNPKLD
                     VNLEKLSFSTDLNKLIPILPLSLKEQIAPLKKTLGSKPSINGKIHYSDSNDKMQIDGG
                     LIIAIPGLNINDIKTDISVQIKKDSTGEITISKCDISALKGVLTGKLNGNLIKKQTTD
                     KPPMGAYFPSLNLSLLMSSASQIEIINGIKFDGLISLDLNLKDYLAKGKLDSKNSNLV
                     YYSGTCKENDYTNCNLMYINRINIDFPFEHNLKITSTKNLIEGNKAKYISTYGQSLPP
                     NFTIQNVIGTHPYIQNDLFKFLQENGIQPGLAARLDYKENVLYMNALRISTLDGFIFG
                     KDILFNLGNTNMEEMEYAATIQIKDIDLKQLLPDKSRKGIADGKIRGDINLSGKNLKD
                     SIGNLNLFLSVFKIGEDFGRSAIRIISPPNLLTDGIISSYSQINKIEIELSKGLVYAH
                     ILFHRSILSTVFMSIENNKISQERMPLANFLNRAQSEVSKYE"
     gene            complement(23040..23381)
                     /locus_tag="PLF66_13100"
     CDS             complement(23040..23381)
                     /locus_tag="PLF66_13100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008594836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HMZ65029.1"
                     /translation="MKIKVILKNDVHIIKIEGPIKAGNEFELGEKIEEYIKKGKAPKF
                     IIDLKKVPFINSAGLGIFLNIYKHVDGLKGRMVFANLNSDIENLMEITKLASVFEIFK
                     TADEALESFDF"
     gene            23730..24059
                     /locus_tag="PLF66_13105"
     CDS             23730..24059
                     /locus_tag="PLF66_13105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65030.1"
                     /translation="MKSKIQRQVVKFLLFFGLVTPFVITYTLPDLSFSTEYNNNYFEL
                     TNKSYSSSDCILSETNDDLEETSFFFLSSFSLFSYNQYSSIASYYNFTVLSKIPFELF
                     SIPPPLS"
     gene            24135..25910
                     /locus_tag="PLF66_13110"
     CDS             24135..25910
                     /locus_tag="PLF66_13110"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HMZ65031.1"
                     /translation="MSFLHILTGFSLFLPILWGIQIGKDFMGLDFPLLFGLCIQAVIG
                     NFILIYARGYETKERGKIIFGYFLFFAGLSGAYLAGKTIWLPVFWEISTLGSLFIYLG
                     QGFSVKAIRSVLALFLASSTSMVFLASWVFLPEGKLGYSFLMLGLLIKAAFSLFHMWL
                     PEAHAGPPAHGSAAYSGLMINLPLLLFVRYMMDWWNEIPLTHYLIPMAGVGVFLGGIT
                     SFFSRDAKKSLAYSTVENSNFLWLFLFVSALWIGSEIEEYNSLGKSFLVLFYLMLLHH
                     SFSKVFQFLSIGYICKKAKSTLIDTCRGVGRISGLSTLELSPGTMSFAVIPGTTGFIA
                     EATLLFLISKTIDLSNVDDSVFFLLAIVLSLSGLVLGSAAHVRLYLPLVLSIPDRKMV
                     AEIESHSHKVPSGVSIALKLLGAGIFIIPILLILPTFIMNKYISCNANSTYECVNILS
                     GKYALLANWIPSYLLVWLFKLSLISAGMTVFYMSLVAFRWAHKIYKRRIWDCGSNYAG
                     PELSIPASVISDPLYHSIGRYFINKDGELYFDNSVINGLVHSLNLGKYWIDKVESGEI
                     AYYILFSALSFLLSLGFIIFLKVHI"
     gene            25920..26789
                     /locus_tag="PLF66_13115"
     CDS             25920..26789
                     /locus_tag="PLF66_13115"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015679172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HMZ65032.1"
                     /translation="MEIINQFNLLLFFIAFPLIVGGIIRKVRARAQGRKGPSILQNFY
                     DISRLFVKRPIDGVHSGFFAEIAPVTVCFAGIIMWSVAIYEWLPFIIIPFFLALQRMA
                     TTAFAMETGTSFGGLGSSREILLSISSEPILLLSILVAQSKLQLGFSWVSIALGTLFL
                     GALMIAVLAELARPPFDDPRTHLELTMVHEAMLLEASGKTMGFFELGYQMKIATLFVL
                     VVRIGLEHSNFVNISIAKPMENVITFLGAIGISILVGYWEAISVRRKWVWVPEVMGIT
                     LLAILVLGTLVKL"
     gene            26799..27431
                     /locus_tag="PLF66_13120"
     CDS             26799..27431
                     /locus_tag="PLF66_13120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020775590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenase"
                     /protein_id="HMZ65033.1"
                     /translation="MTYNFFDLIYLLLVLTGIVILIENRLERVLLLTGLQGFLLAFPV
                     FQVHSVSDPHSWILASMVLVFKTTLTPYVLFWSVKKSKVSVHTKPRFGYLATFFFFIM
                     GLIASLYIVNGLGELPKEVDKIGVIYVFLLIYLGLITFIVRTHWIVLICGFVMIENGI
                     FLLTLILDKGLPFGVEFGAFIDALLVIVAAVALQLRGDSIKQMGVKSSWN"
     gene            27422..28639
                     /locus_tag="PLF66_13125"
     CDS             27422..28639
                     /locus_tag="PLF66_13125"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HMZ65034.1"
                     /translation="MELTSLSIVSTITFILVILTLVFAFTKSDEKVKYWSGLVILLFL
                     AIMLAWWSDDLALQWVFIEATTLIVALLVSMSRTEKSIEVAWKFLLLNSFGLSLAFLG
                     LIILSFGIHSQVTTNINDILNQVSSHQTVLVETGMWLAIFGYSAKLGLFPNHFWVSDT
                     YAESPSQISSIVSSLLPPAVCIALRPLVKMDYQFTSEHFSSSSGLLILGMLTMLYSLW
                     TVFQTNDIRRITAQIALFHSGGLAIFVFLNPPDTIFYFILAANVTVKALLFSAMGIFR
                     IDAGTRKLTDIKPDQGLNKMSTTLYVISVGMAFVIPFSPFFVMDMLFLKLGLQATKYW
                     IIAVPIFSLIFFLVVFNKILPVLNIESRRLLPEYEKVLKIRMAFTIMILILSVCVGLY
                     GIFLFANGGFTNV"
     gene            28632..30086
                     /locus_tag="PLF66_13130"
     CDS             28632..30086
                     /locus_tag="PLF66_13130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010419261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal (Ni/Fe) hydrogenase large subunit"
                     /protein_id="HMZ65035.1"
                     /translation="MSNNSLFDSFEFITGLFYNKRTSTYYKFLQNNESVRMVPLKDAK
                     LQDILKEIRFPIWVLRHSLGVEGGEENYSDVDIESVKKIDDKRKLEISHSMIGNNSIR
                     DLSFNGISVQVAPDSYSHAVGPIHAGVIEPGHFRFAVRGEYIQHLTIRLGFQKRNILN
                     LLKGKTPLQAMSISESISGDSAVAYATAFARIYEESLGLQVSNEVKLIRMVLLELERV
                     AIHIGDIGAMAGDIGYYPLHGVCATDRGVPLGVMETLTGSRFGKGAIFPGEVRLSKNL
                     NLSVLITLAANLINCFRRVEYQFMRAVNSSTFRERLDECGRISRTQVLQNSIIGMPAR
                     ATGIRMDMRYSDPLYQTAKYPFDLNLEREHLNGDAWARFYLRFLEIKASVAWLEKILP
                     ELDLSKAGNGSLQILSKTKFKSGIYFQSVEGWRGSVLAALDINTKGIIQEAYVRDPSV
                     LNWHALELAVRGELIGDFPLNNKSFNLSYVGFDL"
     gene            30083..30907
                     /locus_tag="PLF66_13135"
     CDS             30083..30907
                     /locus_tag="PLF66_13135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004425548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HMZ65036.1"
                     /translation="MMGIYELVNFFKKNTTMDFEKASPINQNARGIPVPSKLATQGSC
                     KNCKVCEANCPTKAIQVKSEVELKIDYGACLQCGICVNVCPSDRLQNSSLVYAFALNR
                     EELKVSYVKGDFVPKEYPTPESILPFQKLTKKRGFNYREVAAAGNNTVECELNASFNN
                     FFDSEGQMVRSVASPKHADAILFSGPVSKNMEGPLQTAWDCVAEPKALIAAGTEAIMG
                     GVFQAGKKPKEPDLYIAGDPPRPDVMLSAFRYLMGQIQYSFQSTLKERLKTLREEK"
     gene            30993..31979
                     /locus_tag="PLF66_13140"
     CDS             30993..31979
                     /locus_tag="PLF66_13140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008589958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent bicarbonate transport family
                     permease"
                     /protein_id="HMZ65037.1"
                     /translation="MSFSIILDNFLNPPVLFFFLGVAAVLVKSNLEMPEALSKFFSMY
                     LLFSIGFKGGVELVKSGFSQEHFLLLLACSVMATIVPIYSFFILRAKLDVHNASAIAA
                     SYGSVSAVTFVTAGAFLHTVNIEFGGFIVAGMALMESPAIVLGVLMDRIFGRGVTESG
                     EKESFSWKELLHEAFFSGSIFLLVGALVIGALTGEHGWNAEKAFADDMFKGFLSFFLL
                     DMGLSAAKRLGDLKKSGVFLVAFGILMPLLNAILGVGLAKLIGAPKGDALMFIVLCAS
                     ASYIAVPAAMRMAIPQANPSLYLPMALAITFPFNIIVGIPLYYYLVTQFIGG"
     gene            31982..32269
                     /locus_tag="PLF66_13145"
     CDS             31982..32269
                     /locus_tag="PLF66_13145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65038.1"
                     /translation="MKTAKKVEIITGSIEFKYIIKVFSENKIAYTAITNISGQGAHGE
                     KSSDEIFGILKNSYLISTCNEQEWDKIKEDVRRVIKTYGGVCMLTDVEVFA"
     gene            32293..32901
                     /locus_tag="PLF66_13150"
     CDS             32293..32901
                     /locus_tag="PLF66_13150"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HMZ65039.1"
                     /translation="MSFIGSIPLGAINLTALQIYLQRDLKSVVYFSFATSAIEFVYSF
                     FAIKGEGLISSNSSLDLFFNYISIVVLFVLGVTSLLSKSKVLSRKSLEEADNPNLPHP
                     YIASLRKGFTLGLLNPLAIPFWIVMIGVLEKQNLIQTKSNFEVLSLMLGIGLGGWICV
                     ICFAFISGKIIQKKEFRLTVVNQIIGVIFLGLGIYQVYKLFK"
     gene            complement(33202..>33773)
                     /locus_tag="PLF66_13155"
     CDS             complement(33202..>33773)
                     /locus_tag="PLF66_13155"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HMZ65040.1"
                     /translation="SERIFPIGRLDKDSQGLLLLTNNGDIVNKILRAENNHDKEYLVT
                     VDKPITDSFISGMTKGVPILGQVTKKCIVKKENTFAFKITLVQGLNRQIRRMCEYFGY
                     NVTKLERIRIMNIHLKGIQGGDYREITEDEFKTLSKLTENSTGTSATGTKSKPSTKRH
                     ILAKQNQHTKSKSHRSNRRRNEPRKKFSK"
BASE COUNT        10770 a         5136 c         5672 g        12195 t
ORIGIN      
        1 agcttcctct aatattctaa tttcctttat catacttatc aatttctatc ttttgcgtaa
       61 ggtcagttat taagaaatga acttatgatg atagatactt tttttaaaac tctgatctat
      121 catgcattta aattattcta ataaaggctt tatattttca gccaaaactt tagcactcag
      181 tttattccct tctgctgttg gatgtccatc ctctggtaaa aaaaataaat atttcttctc
      241 acttcttaat tttttatctt cttcgcgaaa tagttttagt gggttaatat gcattatatt
      301 atgacgttta cagattgctt ctattatatt ttcagctgtg tctcggttat caaattgtgg
      361 ctcatgaatt cttacaaaga ttggataagt ataaacaatg agcttgaaat ttttatcttc
      421 acttagcttt ttcattttca ccagtgcttc ttcccattct ttccacaact cccaagtttc
      481 tttatcatct tcttttgtgt aaggtaaaat attcccctca ttttttaaat aataatgtat
      541 aactaaattt ttaatatcat ccctatcaat ttttgtttga gtaaattttt ttagatcatt
      601 tggccaatct atatgcgata tgctcggttt tattccaaat ttttcaaata caagatcatt
      661 ttgattcatt tctttgattt gttttagcaa aagttctctt tcttttttat acttcccagc
      721 aataaaaaaa cgaattggtc caaaccatcc aatttttttt aacatatcat atgtaaatgc
      781 gtcagtcgca tttctatttt ttaagtctga aatatcattt tcgcaaaagg tcaaaaggac
      841 tacatcagga gaaaactgaa catcgtaatt ttttaagctt tcataagaat ttgtgatagt
      901 ggatgctgtc attcctgcgt ttaatacttc tactttttcg acaccatttg gtttcgattg
      961 atttaatagt tgttctagat gattggaaac agtatcttca ttcgaaactt tccagcccca
     1021 tgtaaaacta tcccctacag aaattactct ttttacgcct tttccttttt ctctttggat
     1081 tgggcttgtt ctcctgtatc cagagtaata agtcactgtt tctccagcaa atttaaggct
     1141 gagtccatct ggtgttgatt ttcgtaattt ggattgtggg aggtaaatga gaatatctcc
     1201 ctccgttaat tctttttcaa aattgggatt gtctaaacga aagtttggat ctaaaaatga
     1261 atcaaatcta aaatttggaa ttttatttat tttacataga gctagaaaaa aaccgtgtaa
     1321 tgctaaaata aaaatactga ttgtaaacaa actaaatgct agaatcttaa atgactgttt
     1381 gtgcaaacta ttaacctcat agaaagtatg aactgatccc gaacacaaaa aagaaaaatc
     1441 taatcttgaa aaaattatgc gtttggttat atcaaatgag attcaaatgc aatctttatg
     1501 gaaaattact tttgtcattt gctatataag tttaaagtta agaaactatt tacacctgca
     1561 agtgaaattt atttccttaa ctttagtttt ttcttctgat tgaatcaaat ggattgaaca
     1621 cttagaaata aataaaatca tttcattttc tatttaaggg gatgtcttct tgtataaatt
     1681 tttttatttc atcggatgca atttgattac cgagtatatt ccaatgagta tctctaggtt
     1741 tgtataaaaa atctgatttt ggatggtttt tcatgattgg atataaatct aaatacttga
     1801 ttcctaagga atctagttta ctccgaaata ttttatttgg aagttccata tcccaatttc
     1861 tatctttata atatttttga tacttgttaa acaagaaggt tccaaattta gagcttactt
     1921 gaacttcttc tggtattata acaactctaa atttaatgtt ctgcttacgg caaatttcat
     1981 ttatctgttc tatgtatttg aatgctttat caatcatcgc tggcagcaaa acgttgtttg
     2041 gtgaaaaatt ctctcgatta aaactaacaa tttctaaaaa tcgttcctct tcaaagttgg
     2101 gacattcatc gcaatactca gtcaatccat taaaaatatt tccttcaaac ttagtttgaa
     2161 ttgttagaaa ataatggaag aaagtgaaca aataggattt cttaaaccat ttttcttttt
     2221 ttggatctat tagatttccg ttctcatcta agtaatcacg aaaggattca ttgaagtcat
     2281 ttccaatata aaaagagagg agtagcatat ccggtttgag ttctaatcct tctcccataa
     2341 attgagaaaa atactctcta ggtcctgtcc caggaattcc catatttaga agatccaaat
     2401 tgtaattttc cttttgaaat gactcttccg acaaagtaag ataattgtat ttataccgaa
     2461 cgccaaaatt aatttcccat ttttcaattg caaataagtg ttcggtctat accaaacgtt
     2521 actccaataa aatccttgtg ggaagtttct tttggcgttt ggtatatagg gaacgactcc
     2581 atatgcaaaa gaatctctaa ttgctatgat tctatattta ttttcctctt ttttattgaa
     2641 ctcaaggtct ttatagccct gagaatttaa tgtaaaatcg cagtcttttg tatgaggttt
     2701 gcccttaaaa cgagtgtagg agttgctgta aaaaataaaa acaggaagaa aaataattat
     2761 aaactctaag aaaaccttcc cctataagaa aggagaatag gattgtaata ctagtaaaag
     2821 agattttttg attttttctt aatttattta ggattttctt tagtatttat aaatcaagta
     2881 tttggaaatt ttagcacgaa ttctagactg tgatttttgg aatcagtaag cgatcgctca
     2941 aatttatttt aaatttctct atatatctaa tttacaaata ggaatctctt taaaacatta
     3001 acactatatg aatatcttag gaatatcttg cttttatcat gatagtgcag ctgccatttt
     3061 aaaaaatgga gaggtcgttg ctgctgcaca ggaagaacga tttacaagga aaagacacga
     3121 tgccaatttc ccaaatcgcg ctattgaata ttgtttggaa actgctaatc tgaaagtcag
     3181 tgatttagac tatgttgctt tttatgataa acccttttta aagtttgaga gaattattga
     3241 atctgcattg agtgctgctc caaaagggct cccaactttt atgaaggcaa ttcctatttg
     3301 gatgagtcag aaactttttg caggggaaga aattaaaaaa caattaggca aaaaagaatt
     3361 taaaggaaag ctaatttttc ctgagcacca tgaatcacat gctgccgctg cttttttccc
     3421 atccccttat ttagatgctg cttttttaac tgtagatgga gttggggaat ggacaactac
     3481 aagttttgga gttggtcgcg acaatcgaat tgaaatacta agtgatatta aatttcccca
     3541 ttctcttggt cttctctatt ctgcgattac ctattacaca ggctttaagg taaactcggg
     3601 agaatataag gttatggggc ttgctccata cggtgagcct aaatatgtga agctaatctt
     3661 ggataacctc attgatttaa aagaagacgg ttcttataaa atcaatctag aatattttga
     3721 ttatgtgggt ggagacagga tgacaaacaa aaagttcgat gagctctttg gtgcgcctgc
     3781 tcgaaagcca gaaacaagcc taacacaacg ggaaatggat ctggctcgat ccattcaaga
     3841 ggttgttgaa atagtagtta tgaaaatggc aagacacatt cataaagtaa caggcatgaa
     3901 atatctttgc ttatctggtg gtgttgcttt gaattgtgtt gccaatggta aaattttaag
     3961 agagggtccg tttgatgaca tatggattca accagccagc ggggacgcag gatctgcgtt
     4021 aggtgctgcc ttaattactc actatcatat taacaatgct cccagaaaag gaacaaactt
     4081 tgcccgactt caaaaggctt cctatttagg acctgaattc agcgaagagt atattgaaaa
     4141 ttattttaaa acaaataata ttccgtatga aaaagttcct aagacagaag tggcaaaacg
     4201 aactgcaaaa gttttatcgg aagaaaaggt agttggatgg ttccaaggta gaatggaatt
     4261 tggaccaaga gcattaggtg ctagatctat tattggagat ccaagatcta ggaatatgca
     4321 atctattatg aatcttaaaa ttaaatttag agaatcattt agaccttttg ctccttctgt
     4381 tttacgagaa gatgtatcaa aatactttga gcatgaaggc gattctcctt atatgttgat
     4441 agtagctcct gttaaagatt cgattcgaat taaaatgact cctgagcagg aaaaactttt
     4501 tggaattgaa aagctaaatg tgcctagatc tactctgccg tcaattacac atgtggatta
     4561 ttctgcgcga atacaaactg ttagccgcga agatcatcct atgtattatg atttgatctc
     4621 agagtttaaa aaattaacag gcgtgagcgt tgttgtaaat acatccttta acgttcgagg
     4681 ggagccaatt gtgtgcactc cagaagacgc atatagatgt tttatgagaa cgaatatgga
     4741 ttaccttgtt gtagaaaatt ttatcttaga taagacaaaa caaccagaat taaaaaatga
     4801 cagtgattgg aagaatgaat ttgaactcga ctaacaaaga agaaacaaaa gcaaaagaat
     4861 taaaatcttt ttcacttggg ggcggattat tattatccgc attagcagga tttttttact
     4921 acaaagaaaa aattgaaata gcttatgtat ttgcagcttt aggattagca tttttgttta
     4981 ttcgtttaat taattttaaa tggattacgc cagtgcatac tgcttggatg aagtttgctc
     5041 atatattagg ggttgtaaat acacggatta ttttatttat agtttttatt ttaactgttg
     5101 ttcctatttc tctcttatta aagattttta gaaaagatat tctagataaa aatcttcaga
     5161 aagaagcggt tacctattgg aacgatagac ctaaaaaaga actaaatata aaacattacg
     5221 aaagacattt ttaaaagggg aaaaatatgt tatctttaat aaaagaattt tttgaattct
     5281 taatggagag aaagaagttt tggctgttgc caattattgt attactcgtg ttattatcta
     5341 tcctattagt tatgacacag ggatcagcga tctctccatt tatctataca ttattttgat
     5401 caatgtatat tgcgacgggg catctcgtgg aaatccaggg gatgcctcga taggcgttat
     5461 agcctacaca gattctacca aagaaaaagt cctttttaca atttcgaaag ctataggcaa
     5521 agaaaccaac aatgtagccg agtggacggc tctccttttt gcacttgaaa agtgcttaga
     5581 gttaggagag aaagaaatat cagtttatct agattcagaa ctagttgtaa aacagatgaa
     5641 tggggtttat aaaacaaaac atccagattt aattgatatc aaaaagaaag tagataagca
     5701 taaattagaa ttttctaaga tagaattcat acatattcca agagaaaaaa ataaggaagc
     5761 cgataagctt gcgaatcgtg ctcttgatga ataaaaaaat aaagcttgca taataattca
     5821 atttaaatac aaagcagatt tcaaaaagga atcaactgat tttgtttaaa tggtatttta
     5881 aaaaaattac aaaattaata actgctgtca tttattctga ttctcggaat aatctttctc
     5941 aggaaatgaa tttgctaagt gtggatatga aatatagaaa gggatactta caaaatttct
     6001 attggatagc tcattcagtc ccaatttgta taattttatt tttgtttaat ctttcccttc
     6061 tatctgatag ttttgaatta aaagatggta aaatactttc cggaactgag gtctctagaa
     6121 ctaaaaagaa tattacaatc aaagaagcta atggtaaact caaaacgatt cctctagata
     6181 aactgaaatt tataacgatc gaaaagaaaa ttgaaattaa gaataaggaa acttcggaag
     6241 agcaaaaaga tatttcagag ctgagaaaaa aaattgaaga attgaataca aagattgaat
     6301 ctttgagtga aaaacaaaaa aaagagcaaa ataataatta taaagaagaa gtcatttcga
     6361 ataaagatat aaaagagcct tctacagaaa aaacatatac ctctactgga ggtgcaaaga
     6421 gtattcattc ctattcagag ccgccggtga gtgatgaacc taatattgag ctaacgcacg
     6481 aaatcgtatc agattttatt tggagaggaa atagttacgg tggagaatat ttaagtagaa
     6541 gaaataacac agcgtattca ggcacctctc agtattgggc atatcaacct aacattcgac
     6601 tcaattctcc tttgaaaggt ctttatatag agttttgggg aaactttgcg ttagtaggta
     6661 gaaatgatcg tgattcagat atgagattaa tgcaagcgag ctctggctct cccgctatag
     6721 atcctaacgt atatttttca aagctaagtg caattcaaac agatcctagc aatactaata
     6781 ttttaaattc aggtctattc tacgatcctc caaataatat cgtaaatagt aattgtagtt
     6841 ctgatggagt aggcggttgc tcgaatccga attattcttt cgttgatcca agatcggttg
     6901 gaaaacacaa agaaaaaaat ggaatggctc gaacggacgg aggttttaca acatttgctt
     6961 ataattttca aaataaaaag tatggtgata taacatgggg ggtttggttt tattatcaga
     7021 tggataaaaa ttcaaaatat tcatgggatg aatattttat tttttgggga cttccttttt
     7081 gggaaggaat tttgaagccg acggtttcct tttatactca atcctcattt gaatctaatt
     7141 cactcaatgc aggaggacac tatctctcct tttcagtttc gcatacattt tttgaaggaa
     7201 agttttttag aattcagcca gcctctaata ttggttataa atatccaaat gataatataa
     7261 atcaaaaatc tggcttttat gatgttactt ctaatttaaa atttttcttt gctgattttt
     7321 tcttcagttt gaatcacgtc tatcgtccca atctatatat gtatgataat gatgtaatgt
     7381 attatacact ttctcaggga tcacaggcac agccaaattt cagtcaatat gatggtaaaa
     7441 ccgttgatcc atctaaacta tatggagtta aaaatgaggc agtgtattca gctattgata
     7501 aaatagattc acctgatata gtaaaaagct atattaaatc agaataccaa tcgcagaaaa
     7561 ttatacaaaa tttattttat ataagttttg gatttaacca taagttttag atttcagaga
     7621 gaatcttttc aaaagaacca aaatagtatt tgagttggtt tttgtgatag aggtagatat
     7681 ttttttttcg catatctttc ccgtttcgaa aagtaatggt ttttaatttt tctgtgcttt
     7741 gaattgtttc attctggatt ttaataaaac ctttggagct tgctttttct ataaatttat
     7801 taaataaaga cgatagttct gaagttaata gattttcagc aatatagtat tcatttgttt
     7861 cttttagttt atttaaaaaa tttgaaattc tttttaataa aatatctttt gaaattccat
     7921 tggcaacatc ggcttgtaat ataaaataag aaaacaggtt acctgctgtg ataacatagt
     7981 tattgccaag tcgagcttta atttcatcgg ctactttaaa ttcatccgca tcttgaggaa
     8041 aatgactatt ttccccaaaa ccaatatgta aaactggttt attctctgaa ataaaatcat
     8101 aggtaaaatt aaaatagttg taggatacat ttgggattct tttaaaaata aaatatgtgc
     8161 ctcttttaat tggatctaaa tttcctgttt ctgaaaactt accctcagga aatagaaata
     8221 ggtttcttcc tttctgaaca ccttctgcga gttgatccca ttgtgaatct acaatgtctc
     8281 tcaattcccc ttgttttaaa agtttcctag catcgtctct aaaagctctc tttacaggaa
     8341 tcccaccgac atatacaagt agaattttta taatttgaga tttgtcgatt aacatgagga
     8401 taaatcggat gaaacctttg ggttcaaatt ctttaattaa aaaatctggt tctataatat
     8461 cttggcgcat tgcaaagcaa aatttaattc tctctggaat gtgtggatat acatgagata
     8521 aggcaacaat gtctagttca gtaacatgat ttgccattag gattgttgga tgcggggcgt
     8581 taacaagttg ttctttcgtt ctgaaatatt tatgaacaga atgaaacatg attcctgtag
     8641 tataggatac aatctttatt aatattctat ataaataagt atcctgcact gcgttcacag
     8701 tgaaagaata gataggtttt aattttttgc aatcacaaaa tctatactta catgacaggt
     8761 gcaggtatta taattgtaac tataaaaata ttttgaaatt catgaaggat actgctataa
     8821 gtattaactt ctattttttt tccccacttt gtttttaaag ttcttcttac actgaagcat
     8881 tctaattctc tcttgagaat ttctagttca aacatatcaa aagatttccc aagaatagtt
     8941 tctatgttct ctctgattgc ttcttttact gtataatgac tgaaaaggta gaaggaattg
     9001 cttgcgaaga aaatttctcc ttctggattt gaaataatta aattcaaatt actaaactga
     9061 aagagtttta taatttctga gatttctttt tcattgccga atttatattt agtgtttaat
     9121 tccaattcat ctttgagctc tagattcatt ttaacctctt tttatttgat gtatctatca
     9181 tccctttttt cattactcaa acattacaaa aaagtaaaga atcgaccatt tatgatgtat
     9241 tgttatattt ttagaacctg tgtattcatt ttgattgcaa tctaaaatga gggattcagc
     9301 tcgcttaaat caaatttaat aaataacaga gtatttttct ttacagaaaa attattgacc
     9361 attcttggta atgatagtat actttttcat aagaaactat ttagaaaggt aggtgatcca
     9421 atgacagaaa aagaagcaaa aaatatagac cctaaattta cggagaaatc tgcaaatgaa
     9481 tttgctcgtt caattgagac gggtgagctt tacgaagagc tgaaaggaaa aaagaaacct
     9541 tctgaggaag atgataattc aaaaaaaaat aaatcataat tgaatctatt catctttata
     9601 atgtcttaaa aaagagagtt caatcaattt ttttttatat tttatattca tgatcccttt
     9661 taaaaattct ataaatctaa tttcaaaata tctaattgca taaaggatta cgttttctag
     9721 taattggatt ttatttgtaa agattagcaa agcctgaaat caaagtctag aatataaatt
     9781 tagaatattt atttaacacc acccaaacgt tttcgatttc tggatttaga ctataaatcc
     9841 atttgagtag gttcccctct ttgactaggg cgaatctatt ctatttatga atgattttat
     9901 aaattaaaat agttagactg aaaaccaatg ttattgtatt tgcaattata attgggtagt
     9961 ctgtttttaa aaatccataa aataaccaaa acgatacacc aatatttaaa agtatataca
    10021 tgtttcttga tatatcttct gtctttttgg tcataacaac tcgtatcatt tgtggtaaaa
    10081 aagaaattgt tgttaaggat gctgctatat atccaatgat tgattcgaat tccatagagc
    10141 catttttgcc tagagacctt ttttgactaa ctctttataa aattcaatcg attccttttc
    10201 tgtattggtt attagattaa cttgaagaaa ggaattttga ctagttagta gcttttttct
    10261 ccataaccat tctgatcccg atctaaatcg actatcgttt tctatttcct caacatacca
    10321 ccaagaaaga aaatattttc tgccatctct ttcaacttcc gcttgtctaa aaaaaatttc
    10381 agaatctatt tggatttctt tttcagattt aaaatggtat cctactgctt cgaaacaaat
    10441 tttgggatga tgaccaagac ctaagaaggg gagatttttt tttaagataa atgtagcaga
    10501 ctgattacga taaaatttag attctttatc ttctgaaata tctatatgaa aactaccata
    10561 gttattcttc caagaaaaat ctgggatgct aagattcttt gctctataga ttaaaagaaa
    10621 gaacaataaa ataggtagaa agtaaatcag taagtattgt ttttttatat ttgtaggaaa
    10681 ataaaatcta acttcttccc taatataaaa aatccaagat aaaaaaaatg gtagtataat
    10741 taaactaaga aagcagagaa ttcctattat gctgtgccat atcgagtttg aattgactcc
    10801 aaataaaata agaattaaaa ttcgagttag gtttgtccat atccagagga taatggatat
    10861 tacccagtaa attgatagga aaataatttt aggtaaagaa atcccatttc taaaatagaa
    10921 ataagaaaaa acggttgcga gtaatacagt ggcaattaaa aattttaatc cctcacatgc
    10981 tggatctact atgtaattta catttcgaaa aaatatttgg tttccactca cagaagtggc
    11041 gcgatctata aaagataaaa taaatccaga aagattagat agaaataatc taagttcaaa
    11101 gcttattaat aaatcatatt tctcaaaaaa aggaggactg atcaaaaaaa gtataaatcc
    11161 aaagttcgaa aaattcattc ccaagagagg caaaaaagat acacagagta tgataccgct
    11221 tatccaataa aaattgaaag agaaaaaata aattgaaaaa atcagtaata tcaatccagc
    11281 aataaattgc aaatgattca aagttctctt ggaatcattc tttaaaaaga aaggaataaa
    11341 cagaagtgga tagaatgaag aaatagaaat aggtgaattc gagttataaa gtatagatcg
    11401 aattagatag gggtttaaaa aaataaatat gaaaagtaga aaaggcaaaa aagcaaattt
    11461 aagcacgttt gaatttgtat ttcccgataa aataaaaaca tagaatacag ataatcgcga
    11521 ttagccattc ttccggttca ggaacatttc cgtgttgaat tttggatgca tcttgcgtta
    11581 gatttacttt actcaaatct tttttatttt tatctatccc aaatcttttg tagtctgatt
    11641 cagattctaa aacaatcaat gatgtaactg gactcacaat acgacctaat tcagccatct
    11701 caaataaatc tttttctaga tagtcttttt tcggatttcc aaagcgttta ccgtatgtat
    11761 ataaaattct tttatataaa acaattctcg ctaataagtc atttcctgaa acgttagttt
    11821 tttcctttct cttttccatt tcaatagtta tactcgaatc aggaaatgaa tatttattat
    11881 ctgtattttc aggtatattt agctttttag ttttaagcgc ttcctttaat gaatcgtaag
    11941 gaatacgaat attctcaatt aatccgtatt cttttaggct gttcaaatag cttgataaac
    12001 tttggttgat cgaatataca aaaagttttc ttttattttt tccgaaaaat tctttgcttt
    12061 tattataaaa aagacttccc tttaattctt ccaatggaat agaactatct tctcgatttg
    12121 taatcactaa gatatctgtt tgatttgcaa ttttgtagaa tggaaaaaga ttaaacttag
    12181 gaagattttg gttttcaata aaatctaaat tctcttcttt attcctagaa taaaaccatt
    12241 cattggtgac taaaacaatt tgagcttcta agttcaaaga cgttaaatca gagtatatct
    12301 tcttccattc ttttttcgag agtgatgaat taaaaagtat ataaatcttt gtcggtaaaa
    12361 aatctacttt ttgtatttgc aaatctgtaa cttttaattc taaatctgaa aactgaaatc
    12421 tcccagttgc ggaaggaagg gcttggactt ggatgctcca tttatctttg tatttaccat
    12481 aaccaaacca acttgtataa gatgagtcag attctttgga taattttttt tgaattgtat
    12541 atccatcgga aattaaatta gagtctttgg cttcaaaaag atttgcctct aatttatgat
    12601 ttgcagagtc taaaatcgga ccttcaattt gaaatccatt gtattctaga gaattcgttt
    12661 gtgtttttaa aggactaata aaaccaattt taaatgtgcg atagttgttt gctaagacag
    12721 gaaaaatttt tactctaagt aaattgtctt ccatccaagt tacataggaa ggatctcgtt
    12781 tttctatacc tacaattgta ttataagctt tttgagcctt tgataaaaat gtgaggcgag
    12841 ctggttcttc ttttccctct atccaaagac tgaggtttgt gcaaatgctt ccttctggaa
    12901 ctttaaatgt ataaatcgct tcttgttgtc caagtccaga ctggttgtag acactcattt
    12961 ttatttctgt gtaagctaat cttgcttttg gatagagtcg aatttttgac tctatatcag
    13021 aggttaataa tgaattgccg ttccataatc gatttagact aacgtgtgtg tatccaaata
    13081 aaagtcttaa taagtgttct ctatcctcat tgagtaaatc tgattttttg gaaatattag
    13141 aaacaacgaa ggcaaaagga tcaaaaagtt tgtcggaacc aaaccaatta aataggactt
    13201 ctctgttatt ggattctggt tgcagatata attcacttat gtgattgact ggaagtttca
    13261 ttccaagaga agcccaggca ggtaggtcat catcgattaa gcttcttttg gagttgatca
    13321 tttttgtttc taaaaaatga ttccgaatga taaattgacc tttgttccat tcactttgaa
    13381 accatataaa gtagatagac gttaagatta atactaaata tgttaaatat tttgaaaagg
    13441 tatattgtag ataatactga ttatccttaa gttcaacaac aatacaatag agcatatata
    13501 gaaaacctat aaatgccatc agaggagtat aaggaaggat accaattcca aatataatta
    13561 aaacccatcc tatcatcata gcgggaatca taattaatac aaaacaaaat gagagaattg
    13621 ctccaatgat cagtattgga gggaaaaata aaagaatcca ctttggaata tttttataaa
    13681 atatggaagc aaataaagtg aaaaatgtga tccacaaagt aatggtggtt ctaggtgtaa
    13741 atggagagaa aatttcatta ttcaaaaaat acttttcaac aacaaaggca gtcccactca
    13801 aaaagagagt aattagtata aaaccagttc tttttttttc taatagaatt cgaatgatta
    13861 aataaaataa aaataaagaa aaaataccac caatgaagag tgcaaagggt tgtaatcctc
    13921 taaagtgagt cgattcaaga tgtttaaagg ttacaataga aataaaacta aacgttacga
    13981 gtgctatgat ccaataaagt atccaaacat atctaggatt ttcatactta aagattttta
    14041 cttctttcgt aaaatagttt ttgattaaat cccacataat taactacaga ttatttcgat
    14101 ctttccttga ttcaatctta aaattctctt tatattcaat tccaataaat gtcatatcat
    14161 cttcttgggg aagatttcct ataaaatcgt ctacttgttg aacaacagaa tttaaagttt
    14221 cactcactga acttttggca tgagattgca agatttccca aagcttttct tccccaaatt
    14281 catttctcat aaaattgaat tcttcaaaaa ttccatctga ataaagaaaa agtttattcc
    14341 cagattcaaa atctaattcg gtttctcggt attttacatt ttctttaaca ccgatcatct
    14401 ttccagaaga actcaatttt tgtatttgct catttctaat aagtatttga ggtggatgac
    14461 ccgcagcggt atatactaat ttgcctcgca atggatcaat atctagtaga atacaagtaa
    14521 agaaagcatt cactgataag aattttctta aaaattcatt gtttagtttt tccattattt
    14581 ctgctgtatt taaagggctg atctttaaag tttcatattc actctttatt gccattgtaa
    14641 tcaatgctcc ctgagtccca tgaccagttg catctgctaa gaaaattctt attttataag
    14701 gattgatttc tgtaatatca aagaaatccc cacccacttc atccattgga ctataaaatg
    14761 tagaaatatt tagagaatga attctttcta aattttttgg aaggatacta tcttgaactt
    14821 tttttgcgag cgccaagtct ttttttattt ttcgaaggga aagatttaac tcttctgtaa
    14881 tttgcattaa attattattt aacaaatatt gctgctttaa tgattcatcc aattcttttg
    14941 ttctttcatt tactctattt tctaagtctg agtaaagtct tgcattttcg attgaaattg
    15001 cggattgagt agaaatcgtt acgagtaatt caacgctatt ctttttaaaa gcataggacg
    15061 ttaagttatt ttctaaataa aaaattccaa ataatccctg tttatgaaaa atcggaaagc
    15121 atagaattga tttagaatta ttttttagaa cataagggtc ttttgaaaac cgttcactct
    15181 tggctgcgtc atttaataca atgtattcat taaagcgaac agagtagttt ataatagaaa
    15241 ggggcaaatc ggcattttcc actggaacag attgtaaaac tgatatatta tttttatcaa
    15301 aagatgcctc tgcttcaata aagagtttat cgtctttttg taaaatcagg actcctcttt
    15361 ccgctccagc attttcgatt aatattttaa caagtgattc aagtagttta tctaataaga
    15421 tttcacggga gattgtttcc gaaactttta ccatactagc aaggttaaaa gactcactat
    15481 cgcctctgtg tgttataatc gaagtgcttt ctgtttcact atttaaatcc tttttataga
    15541 tattatctac aattcctgga tacttttctt ctaattgttt tacttttcta tccgctcccc
    15601 aaatatgata gtaataaagg gcagagttta gatatgtttt cgcaatatct aatttatcta
    15661 gttctaaata aaactttcca gtaagctcgc ttattactgc tgcatcgttt atatattcac
    15721 tttctttgct tagctgagag gctaacgcat agttatccat cgctgaaaga atttgacctt
    15781 ggaccctatt tcgctctgcc tcaattaaat ggtatctatg taaaaaattt gctttcgaaa
    15841 actttgacca tttttttaat ttacgctgat tctttgctac ttttgaaaga taatttcttt
    15901 tttgtagaat atttccttta tcatattcct gcaagagggc aatagattct agaaaaacta
    15961 aaaaagtata cactgctaaa gatcgcatag atccagaata agaagtcgct agttttgctt
    16021 cttgaatcga ttctttatat ctaccgaaaa tgaaattaat atataaattg aaaaagtgat
    16081 aatagaattt atttgcttcg attgtatttg gatctaattt attatctcgg agtatccatt
    16141 ctgctttgtc taaagttgaa aatgaatcct ctttatcctc cataagagta accattgtta
    16201 aacgaattac ttccatttgg tagcttgagt tttgatgttt atatgattca aaggatttta
    16261 atccagttgc gacttctttt tctagcggcg caagttcttt tcctgcaaag agagcatgaa
    16321 tagaatattg tattaatcca taaaaagcaa attctagatc tcctgtttca tgtccacttt
    16381 tgtagcactc ccataaaggg tctaacgtca atcgaatatg atccttccaa tgcacaataa
    16441 aggaattaac catatgcaat atttttgtct tattttttat cccgcctatc tcttcataaa
    16501 tattaagagc aagcattcca aactgatatc cttggtcaat ctgcccaagt gttccacaca
    16561 aaataattcc gtatgcagaa aatgaaaatg gagtatgttg agtatagccg ctagtcaaag
    16621 aaagatagac attttctaat attaataatg gaagtaaatg aggagatgag aagtaggcgg
    16681 cagacccaat gtttgatatg agccgcattg ctgcaatctt tcttggatct gtcgcttcat
    16741 ttagtttacg aagagagtat attgttttat ttctaagtac gaattttacc ttgagaatac
    16801 ttaatagaac atgataaatt ttcggagaag atggaaaatt gattttaatt tcttttaata
    16861 ttttaattgt attattgatt gcggctatcg gatctccgga tgtgattaaa tatttatttc
    16921 taatttcatt tgctcgggct ttaattaaaa tatcttcatc ttgttctagt ttggtcttct
    16981 gaagaaatgt ttccagttca acagaacgat ttaatagata ggcaacttca gcggcttcta
    17041 gatataaatt tacctttaat tcttttttct ttttttccgc agaggattca aagaaataaa
    17101 taccctctaa aaagaaattc aaggctaaat cgtatgccgc tgaggctttt gctttccttc
    17161 ctgcttctaa atttaattct atgaagcgaa ttctagaagt atctggtgag ataaagtttt
    17221 ttccaagatt taagtgattg ataatagaaa ataaatcatt tttaatttct tcctcagaga
    17281 gagttgttag taatttagag gctatttgtg aatggagttg taattttttt ttattctcta
    17341 ataatgagta agctgctatt tgcacttttt catgcaagaa tttataagaa ttaccggaag
    17401 gtaggataag acctgaggca acagctccaa tgagggattc ttcaatttga aatctattat
    17461 atccagttat cattgaaagc atttcacttg tgaatgaatt tcctagacag gatgcaatct
    17521 gaatcaattc ttccgttgtg gaatgaagca atttcagatt tgcagttacg atgtctgcca
    17581 tatcatcatt tatatttaaa ttgcggataa tgtctatttt ccacatccaa acaaaaccac
    17641 cctctgattt ctctattggc tgtagatact tttctaaata tatgaacttt agaaattcat
    17701 gaacgaaaaa aggattcccg tttgttttaa aaaaaattac atttgctaat tctttacaat
    17761 cctctttcgg ataattaatt gtttctgcaa ccaaatcgat cacatcttca gtctttaaat
    17821 tcaataattc tagatctggc acatctgaat atttttcttt tatactagaa agaaaaatat
    17881 tttgactttc ctctttattt aaataggagc aaattaagag aatattttta ctatgaatgt
    17941 tattaataaa atattcaagt aaatgaaaag agtcagagtc cgcataatcg atattatcca
    18001 aaaaaattaa aataggttgg cggctatatt gaaatatatg aataaaatca cgtaacgtta
    18061 aatgaaatct attatttgtt tcgttctcat ctaaatcgtc tagatctttt aattttccag
    18121 taattatctc caactcaggc atagcattgg ttaatagctt tgcatttact ccgagtatat
    18181 ttaagattcg ttctctccaa gtttcaatgt atgctttgct acctaatagg atttgtctta
    18241 gaaactctcg tgatgctgtt ataaaggcag agtagggaat gtttaaattt gcttcttcat
    18301 actttccata tataaaaaaa ccatttgagt ttagaaccgt tagtttcata tcattcacta
    18361 gagctgtctt tccaactcct ggttgtccat gaataaatag catctcactt ggtccgttcg
    18421 aaattcttct gaatgcatct agtagaaatt gcttttcttt atcccttcca aagagtttta
    18481 gaggtatttg cagttttccc gaaaaatcat tttttcctat ctctaaatag aatggagaat
    18541 tttgttctgc tttcagatgg aagtcgagta attttgataa gtcatgacat attccacttg
    18601 cagactgata tctattttca ggatccttag atagaagttt taaaattaat tctgagagtt
    18661 tttttaatag aaaatcattt cctgggaagt tttgagttgc ttttagccaa ggggagactg
    18721 gacttttcgc gatatgagaa tggattacat ccatagaatc atttgattca aatggtagat
    18781 ttcctgtaag aagttcataa aaagtaacac ccaatgaata aaaatctgtg cgatagtcga
    18841 taggtctatt cattcttcct gtctgttcag gggaaatata aggaatactt ccttctagat
    18901 aatcgatttg catattttca atattttctt tgctgtaaat agaggaaatt ccaaagtcta
    18961 tgatttttaa ctgaaagcta cttggttcta ttaaaatgtt taatgggttt atatctttat
    19021 gaataattcc atttttatga atatgattga gagcttctgc aatggataaa aaaattttta
    19081 ttaatctacc aagatcaatt cgctcttgca caagaaaaga tttgagcgag attccattta
    19141 tatcttcgaa tacgattgca tatctcgcac catcctttaa taactgaaaa gatttaagag
    19201 caccttcaat ttgaagtctg gaagtaattt cgaactcttt gcgataggcg gcaatctctc
    19261 tgttggtagg taactcactt ttatgagttt ttattattac gctagattga tctttagtgt
    19321 aaactccttt gtaaataatt gtatgttgat tttcaaaaat cttttctttt atgttaaaat
    19381 caggaaatga tagcataggc tattttttga tccagtttcc tttagacgac tcgtaatatt
    19441 ctcccgcttc cagtgtgtaa taattagaaa gcaaaatttt ttctctaagt tttgattgtt
    19501 cttctttact tctttctgtt ttactcagtg aaagcattct tgtttcataa atgcgaactc
    19561 ttgcatcgtt gattaacttc atgacattct caactcgttt ctttttctcg ggattagaat
    19621 actcatcttg cgtaggctta tcgagagtaa ccgctggctt ctgcaataat ccatttagtc
    19681 cttcccctaa aacgcctttt gttctataag tttttacttc aggagctaga taggcaatca
    19741 ctccaagaag agatgagtag tctttatctt taaattcact ctcttcatat aggattgaat
    19801 cttttttcca ttcatcagaa ccagaagagg atgttttaat tgaggagata agccatccat
    19861 ctttttctaa ttctttatct tcaccaacca tttgtttttc ggctgctgtt tgagactgag
    19921 taaatgtgat aggaggggga gttaatatgc aatttacaaa actcaaaaag aaaataaata
    19981 ttgtgtttct attcatactt ggagacctca gactgcgctc tgtttaaaaa atttgccaac
    20041 ggcattctct cttgagagat tttgttattc tcaatgctca taaagactgt agatagaatc
    20101 gatctatgaa ataaaatatg agcatagact aagccttttg acaattcaat ttcaatttta
    20161 ttgatttgag aatagctact aatgatgccg tctgttaata aattaggtgg actgataata
    20221 cggatagcac ttcttccaaa atcttctcct attttaaata cgctcaaaaa taaatttaaa
    20281 tttccgattg aatcttttag gtttttacct gacaggttga tatctcctct aatttttccg
    20341 tccgcaattc cttttctaga tttatcagga agcaattgtt ttagatctat atctttgatt
    20401 tgaattgtag ctgcatattc catttcttcc atatttgtat ttccaagatt aaataatata
    20461 tctttaccaa agataaaacc atctaaagta gaaattctaa gtgcattcat atacagaaca
    20521 ttttctttat aatcaagtcg agctgctaat ccaggttgaa ttccattttc ttgaagaaat
    20581 ttgaataagt cattttgtat ataaggatga gttcctatca cattttggat tgtaaagtta
    20641 ggcggaagac tttgaccgta agtgcttata tactttgcct tgtttccttc tattaaattt
    20701 ttagtagaag taatttttag attgtgctca aaaggaaaat caatgttgat ccgatttata
    20761 tacattaagt tacaatttgt ataatcattt tctttacaag ttccagagta ataaactaga
    20821 tttgaatttt tagaatctag tttgcctttc gctaaataat ctttcaagtt tagatcaaga
    20881 gaaattaaac catcaaactt tataccatta ataatctcga tttgggaagc tgacgacata
    20941 agaagagaaa gatttaagga tggaaagtag gctcccatcg ggggtttatc ggttgtttgt
    21001 ttcttgatta ggttcccatt taattttcca gtcaaaactc ccttgagtgc tgatatatcg
    21061 cattttgaaa tagttatttc gcctgttgaa tctttcttaa tttgaacaga tatatccgtt
    21121 ttgatatcat ttatattaag ccccggaata gcaataatca atccaccatc gatttgcatt
    21181 ttatcatttg aatcggaata gtgaatcttc ccatttatgc taggtttaga acctaacgtt
    21241 tttttgagag gtgcaatttg ctcttttaag ctgagcggaa ggattggaat tagtttattg
    21301 aggtctgttg aaaatgacag cttttctaaa ttaacgtcga gcttagggtt catagataat
    21361 tttccatttg ctcctaaact gagagattca gacatttctg gattttttat ttttgctatt
    21421 agattattta cttctaatga ctgaaaagaa aatttttctc caagggcaac tttcccaaga
    21481 agggaaagat ctacatgatt tattcctgat ttactaccgc tgacagaata ttcaagagaa
    21541 cgaacttgag aatctagttt tgtatttaaa ttggaaaaat ttgttccttc tatatttata
    21601 tttccagaaa gatttcctga gactgcattt gtgaaatttc gtaagaatag attttttaaa
    21661 tttaactgag cagaaatatt tttttcattc tctagctgtg cctttaggtt tgctaacatt
    21721 gtattataag aaagatctgt ttttaattct accgaattga gtattggaat tggattttct
    21781 tttgttggtt ctttttttgt ggataaatct aaatttgcca aagcatttag gtttaagtaa
    21841 ggaattgagt gtgatttgga tttttgtttt agaagtaaat cttttccctt aatatctgct
    21901 tttattgtca aagagttcat agttccattc acgttaatag gggctaactc taatttgcct
    21961 ttaaagtcca aatctggtag tcctggaatt tgtctgtaga tggaatttac aggagagaga
    22021 tctaatttag attcgtataa atctatcgca aactctcttt cttgcgtcat tactttgttg
    22081 atttttcctt ttgctcgaat ccaatcatcc tgatcaaaat gtatatttag attttctaaa
    22141 actagactat cgtcaatggg taaataattt aaatcataac tagcttcaaa ggcaaagcta
    22201 ggaagctttc tatttttaac tgttaattca atttgcttag aaccaaattt tagattagaa
    22261 tgaaaggcgg taggattttg atttgaatct agatatatgg caagtgacgt tgagagtcct
    22321 tgcttgattt gattgtgatt atcttgataa aaaatttgta agtcacgttt aggattgaga
    22381 gaaatatcta tggtttcaaa aatattcacg atacttgcat ctaatggaat ttttcgaaat
    22441 ctttttgtag cgattaggat tttaaggtcg atgtcttttt cttctgtcag aaaaaaatta
    22501 tctccttttt ctttttccat tctaaaactt aggtcttgta taaagaaatt taaataggcg
    22561 ctgactggca aatagaggtt tatttcttct ggaagtggtt tagattcttt aggcttttct
    22621 tcctttgttg cgtttgaaac aaaaagctca gcaatgttta gtttattatt tctttctaaa
    22681 atttgaataa agggctttgt aagagatatt tctgaaagtt ttaatttacc tagaaataga
    22741 tagggtagat tataaaggaa tgcaactcta tcacaagaaa ataaaatttc tttattaaag
    22801 ttttcaccgg aacgaatgac tatagaatca atttggattc cataaaaaag agaaaattgc
    22861 tttacctcac cttcaaaact tcctgtcgaa tagtttttga agatgtaggt aaataggtat
    22921 ttgccgctaa atacattaaa tatactttta taaagtatta ggaaaaataa aaaaaagaga
    22981 aaagattttt tcttaaggaa acttttaaga gtttgtaaat taacactact cacagtagtt
    23041 tagaagtcaa aagattccaa tgcctcatcc gcagttttga aaatttcgaa aacacttgct
    23101 agttttgtaa tttccattaa attctcgata tcagagttta aattggcaaa aaccattctg
    23161 ccttttaatc catccacatg cttatagata tttaaaaaga taccaagacc tgcagaatta
    23221 ataaaaggaa ctttctttaa atcaataatg aactttggtg cttttccttt tttgatatac
    23281 tcttcaattt tttcacctaa ctcaaattca ttgccggctt taataggacc ttcaatttta
    23341 ataatatgga catcgttttt taaaattact tttattttca taaccctaat tctttaattt
    23401 agatacaatt gctatcgaag ttctatttta caatgcagaa tatttttgca gttcatatct
    23461 attctgatat tatataacct actgagcagt gaattttttc attttgcaaa ataagctgta
    23521 aaaaacatta taaaataaga aaacatctat gaatgtgttc taattttcat aaatgataca
    23581 atccaatgta aaagaataac atatataatc ctacacttga atctatcaaa tagattcgat
    23641 ttttgtctgt ataagtaaag tattttttaa aatgattgtt tttttttatt cttatttcta
    23701 aatgacagta ggatttgtaa tacaaagaaa tgaaatcgaa aattcaaagg caggtagtta
    23761 agtttttact cttctttggc ttagttactc cttttgtcat tacatacact cttcccgatt
    23821 tatctttttc aacagaatac aataataact attttgagct tacaaataaa tcttattctt
    23881 cctcggattg tattctctcc gaaacgaatg atgatttaga agaaactagt tttttctttt
    23941 tatctagctt ttctcttttt agctacaatc aatattcatc tattgcttct tattacaatt
    24001 ttactgtatt atccaaaata cctttcgaat tatttagtat acccccacct ttatcctaat
    24061 ctcgatttat ttcaattagc aaaaacatta ggagtccgag tgaaatttta attttacatt
    24121 ttaggaaaaa aattatgagt tttttacaca ttctaacagg cttttctctc tttcttccca
    24181 ttctttgggg gattcagatt ggaaaagact ttatgggcct agattttcct ctcttgtttg
    24241 gcctttgtat tcaagcagtc attggtaact tcattttaat ttatgcaaga ggctatgaaa
    24301 caaaggaaag agggaagata atttttggct atttcctatt tttcgcagga ctttctggag
    24361 cgtatctagc cggaaaaaca atatggcttc ccgttttttg ggaaatttct acacttggct
    24421 ctttattcat ttatctggga caaggatttt cagtgaaagc aattcgaagt gttcttgccc
    24481 tttttttagc aagtagcact tccatggttt ttttagcttc ttgggttttt ttgccagagg
    24541 ggaaacttgg ttattcattt ttaatgcttg gtcttttgat taaagcggca ttttctcttt
    24601 ttcatatgtg gcttccagaa gctcatgcag gaccgcctgc acatggttct gctgcttatt
    24661 ctgggttaat gattaattta ccgctattgc tatttgttcg ttatatgatg gattggtgga
    24721 atgaaattcc attgactcat tatttgatcc caatggctgg ggtgggagtg tttttaggag
    24781 gaattacatc tttctttagt agagatgcca aaaagtctct tgcttatagc accgtagaga
    24841 atagtaattt tctttggctt tttctatttg tatccgctct atggattggc agtgaaattg
    24901 aggaatacaa ttctctagga aagtcctttt tagttttatt ttatttaatg cttcttcacc
    24961 actctttttc taaagtgttt cagttcttgt cgattggata catttgcaaa aaagcaaaat
    25021 caacactcat tgatacctgt agaggtgtag gaagaatttc tggtctttct actttagagc
    25081 tgagtcctgg aacaatgagc tttgcagtca ttcccggcac aaccggtttt attgcagaag
    25141 caaccttact ctttttaatt tcgaaaacca ttgatctctc caatgtagat gattctgtct
    25201 tctttttatt ggcaatcgtt ctaagtcttt ccggtttagt attgggaagt gcagcacacg
    25261 ttagactcta tctcccactt gtgttatcta ttcctgatag aaaaatggta gccgagatag
    25321 aatcacattc acacaaagtg ccaagtggag tttcgattgc actcaaatta cttggtgcag
    25381 gtatttttat aatcccaata ctcttaatcc ttccaacttt tatcatgaat aaatacattt
    25441 cctgtaatgc taattctact tatgaatgtg taaatatttt atctggtaaa tatgcgttac
    25501 tcgcaaattg gattccttct tatttattag tttggctttt taaattgagt ttgatttcag
    25561 ctggaatgac agttttctat atgagtctcg ttgcatttag atgggcacat aaaatataca
    25621 aaagaagaat ttgggattgc ggtagtaatt atgcaggacc tgagttatca attcctgctt
    25681 ctgtaatttc tgatccattg tatcattcta taggaagata ttttataaat aaagatggcg
    25741 agctatactt tgataatagt gtgatcaatg gtttagttca tagtttaaat ttaggaaaat
    25801 attggatcga taaagttgaa tctggagaaa tcgcttatta tattctattc tctgcattgt
    25861 cctttctact atcgcttgga ttcatcattt tcttaaaggt tcatatatag gatttgcaaa
    25921 tggaaattat aaatcaattc aatctacttt tattttttat tgcattccct ttgatcgtcg
    25981 gcggtatcat tcgtaaagtg agagctagag cgcaggggag aaaaggaccg agcatacttc
    26041 agaattttta cgatatttct aggcttttcg ttaaacgtcc gattgacggt gttcattcag
    26101 gtttttttgc agaaattgca cctgttacgg tttgttttgc aggtatcatt atgtggtctg
    26161 ttgctatcta tgaatggttg ccttttatta tcatcccatt ttttctggct ttgcaaagaa
    26221 tggcgactac cgcatttgca atggagacag gaacttcttt tggaggttta ggttcctcta
    26281 gagagatttt actttccatc tcttccgaac caattttgct cttaagtatt ttagttgctc
    26341 aaagtaaatt acaacttgga ttttcttggg tgagtattgc acttggcaca ttgtttttag
    26401 gggctcttat gattgcagtt cttgctgaac tggcaagacc tccatttgat gatccaagaa
    26461 ctcacttaga acttactatg gttcatgaag cgatgctttt agaagcttct ggaaagacga
    26521 tgggtttttt tgaattagga tatcaaatga aaattgcaac cctctttgtt ttggttgttc
    26581 gaattggttt ggaacattcc aattttgtaa acatttcaat tgctaagcca atggaaaatg
    26641 taatcacctt cttgggagca attgggatat ctattttggt tggatattgg gaggcaatta
    26701 gcgttaggcg taaatgggta tgggtgcctg aagtaatggg tattacatta ttagcaattc
    26761 tagtattggg gacgctggtt aaactgtagg ggctttttat gacatataat ttttttgact
    26821 taatttattt actactcgtt ctcaccggaa tagtgatact cattgaaaac agattggaaa
    26881 gagttttgtt gttaactggg ttacaaggtt ttctattggc atttcctgtc tttcaggtac
    26941 atagcgtatc agatccacac tcatggattt tagcttcgat ggttctagtt tttaaaacaa
    27001 ctcttacgcc ttacgttctt ttttggtcgg taaagaaaag taaggtgagt gtccatacga
    27061 aacctaggtt tggatatcta gctacttttt tcttttttat aatgggactt atcgcatctc
    27121 tctacatagt aaatggtctt ggggaattgc caaaagaagt agataagatt ggagtcatct
    27181 atgtatttct attaatttat ttaggtctta ttacatttat cgtgcgaacc cattggatcg
    27241 ttttgatttg tggttttgtg atgatcgaaa atggtatttt tctattaaca ttgattttag
    27301 ataaaggact tccattcgga gttgagtttg gagcgtttat tgatgcgtta ttagtgattg
    27361 ttgcggcagt agcactccaa cttagagggg attcaattaa acaaatgggg gtcaaatcat
    27421 catggaatta acttcattaa gtattgtatc aacaattact tttattctcg ttattctaac
    27481 gttagtattt gcattcacca agagtgatga aaaggtcaag tattggtctg gattagttat
    27541 attacttttt ttggcaatta tgcttgcttg gtggtcggat gatctagcat tacaatgggt
    27601 ttttattgaa gccacaactt tgattgttgc ccttttagtt tcaatgagta gaacagaaaa
    27661 gtctatagaa gtagcttgga agtttttact tttaaattcc tttggtttga gtttggcatt
    27721 tttgggattg attatactga gttttggaat tcattcacaa gttactacaa atataaatga
    27781 catcttaaat caagtaagtt cccatcaaac cgttcttgtc gaaacgggaa tgtggcttgc
    27841 gatctttggg tatagtgcca agttgggatt atttccaaac cacttctggg tgagtgatac
    27901 atatgcggaa agcccaagtc agatatcgtc tatcgtttct agtttacttc ctccggctgt
    27961 ttgtatagct cttagacctc ttgtaaaaat ggattatcag tttacatctg aacattttag
    28021 ctcctcaagt ggacttttaa ttcttggaat gttaacaatg ctttatagtc tatggactgt
    28081 ttttcaaaca aatgatattc gtcgtataac agctcagata gctttgtttc atagtggtgg
    28141 acttgctata ttcgttttct taaatcctcc agacacaatt ttttatttta tattagcggc
    28201 taacgttaca gtgaaagcat tattattttc tgcaatggga atatttagaa ttgatgcagg
    28261 cactagaaaa ttaacagata tcaaaccaga tcagggactc aataagatgt ctacaactct
    28321 gtatgtaatt tctgttggga tggcatttgt aattcctttc tcaccgtttt ttgtaatgga
    28381 tatgctcttt ttaaaacttg gtctgcaagc gactaagtat tggattattg cagttccaat
    28441 ttttagttta atattctttc tagttgtatt taacaagatc ttacctgttt taaatattga
    28501 gtcgaggcga ttacttcctg aatacgaaaa ggttctaaaa attagaatgg catttacaat
    28561 tatgatttta attttaagtg tctgtgtggg tctctatgga attttccttt ttgcgaatgg
    28621 aggatttacc aatgtctaat aacagtttat ttgattcttt tgaatttatt acaggtctat
    28681 tttataataa acgaacctcc acttattata aatttttaca aaataacgaa tcagttagaa
    28741 tggttccttt aaaggatgca aaattacagg atatattaaa ggaaattcga tttccgattt
    28801 gggttttaag gcatagccta ggggttgaag gtggagagga aaactattca gacgtagata
    28861 ttgagtctgt gaaaaaaatt gatgacaaac gaaaattaga aatcagtcat tccatgattg
    28921 gaaataacag tattcgagat ttgagtttta atggaatttc tgttcaagta gcaccggata
    28981 gttattctca tgctgtcgga ccaattcatg caggagtcat agaaccagga cattttagat
    29041 ttgcagttag aggtgaatac atacagcacc taacgatccg ccttggattt caaaaaagaa
    29101 atattttaaa tcttttaaaa ggaaagactc ccttacaagc catgtccatt tctgaatcta
    29161 tctcaggaga tagtgcagtg gcttatgcaa ctgcttttgc tagaatttat gaagagtctc
    29221 ttggtcttca ggtttcgaac gaagtaaaac taattagaat ggttctatta gaattagaga
    29281 gagttgcgat tcatattgga gacattggag caatggcggg ggatattggg tattatcctc
    29341 tacatggtgt ttgtgctact gatagaggcg ttccactcgg tgttatggaa actctaactg
    29401 gctcaagatt tggaaagggt gcaatctttc caggagaagt taggctcagt aaaaatttaa
    29461 atctatccgt tcttataaca cttgcggcga atttaatcaa ttgttttaga agagttgaat
    29521 atcaatttat gcgcgcagtg aattcatcta cctttagaga aaggttagat gaatgtggaa
    29581 gaatttcaag aacacaagtt ttacaaaaca gtattattgg tatgcctgcg agagcgactg
    29641 gaattagaat ggatatgcga tattccgatc cactctatca aactgcaaag tatccattcg
    29701 atttaaactt agaaagagaa catctaaatg gcgatgcttg ggcacgtttt tatttacgtt
    29761 ttttagagat caaagcgtcc gtagcatggc ttgaaaaaat tcttcctgag ttagatctat
    29821 ctaaagcggg taacggttcg ctacagatat tgagtaaaac taaattcaaa tcaggaatct
    29881 attttcaaag tgtagaaggt tggagaggat ctgttcttgc tgctttggat ataaatacca
    29941 aaggaatcat tcaagaggca tatgtgcgag atccttctgt tttaaattgg catgcattgg
    30001 agttggcggt tcggggagaa ttgattggag attttccttt gaataataaa tcttttaact
    30061 taagttatgt ggggtttgat ttataatggg aatttatgaa ttagtaaact tttttaagaa
    30121 aaatacaaca atggattttg agaaggcatc gcctatcaac caaaacgcca gaggaattcc
    30181 tgtcccatcg aaattagcaa cacaaggctc ttgtaagaat tgcaaagtct gcgaagccaa
    30241 ttgtcctaca aaagcaattc aggttaaatc agaggtagaa ttgaaaattg attatggagc
    30301 ttgtttacaa tgtggaatct gtgtaaatgt gtgcccatca gataggttac aaaattcttc
    30361 ccttgtatat gcatttgcac tcaatcgaga agaactaaaa gtatcttatg taaaaggaga
    30421 ctttgttcct aaggagtatc caactccaga atcaatttta ccatttcaaa aattaaccaa
    30481 gaaaagaggc ttcaattata gagaagttgc agcagccgga aataacacag ttgagtgcga
    30541 actaaatgct agcttcaata atttttttga ttcggaaggt cagatggtta gaagtgtggc
    30601 ttccccaaaa cacgcagatg caattttatt ttctggacct gtaagcaaaa atatggaagg
    30661 acctttacaa acagcttggg attgcgttgc tgaacctaaa gcgttaatcg ccgcaggaac
    30721 agaagcaatt atgggaggag tctttcaagc aggaaaaaaa ccaaaagagc ctgatcttta
    30781 tattgcagga gatcccccta gaccggatgt gatgctcagt gcatttaggt atttaatggg
    30841 acaaattcaa tattcattcc aatccacctt aaaggagaga ttaaagacac taagagaaga
    30901 aaaataactt ataaatatat aaactaacgt attagagata aataaagcaa aagcaatcga
    30961 ggaataaaaa taaagtagga gttacttcac gtatgagttt ttcaattatt ttggacaatt
    31021 ttttgaatcc cccagttttg ttcttttttt taggggtagc agcggtctta gtaaaatcaa
    31081 atttggaaat gcctgaagca ttgtctaaat tcttttctat gtatctttta ttttccatcg
    31141 gatttaaagg aggagtggaa ttggttaaga gtggctttag ccaggaacat tttttattat
    31201 tgcttgcttg tagtgttatg gctactatcg ttccaattta ttcgtttttt atattaagag
    31261 ctaagttaga tgtgcataat gcgtctgcga tcgctgcttc ttatggttcg gtgagcgctg
    31321 tgacatttgt tacagctggt gcttttttac atacagtaaa tattgaattt ggtgggttta
    31381 ttgttgctgg tatggctctt atggagtccc ctgcaattgt tcttggtgta ttgatggatc
    31441 gaattttcgg gcgaggagta actgaaagtg gcgagaaaga aagtttttct tggaaagaac
    31501 ttttgcatga agcttttttt agtggttcca tttttttatt agtgggagcg ttagtgattg
    31561 gagctttaac cggtgagcat ggctggaatg cagaaaaagc ttttgcagac gatatgttca
    31621 aagggttttt aagtttcttt ttacttgata tgggtctttc ggctgctaaa agattgggtg
    31681 atttaaaaaa gagtggtgtt ttcttggtag catttgggat tttaatgcca ttattaaatg
    31741 cgatacttgg agtgggctta gcaaaattaa ttggtgcccc aaaaggggat gcacttatgt
    31801 tcatagtgct ttgtgcttct gcttcttata tcgcagttcc tgctgcaatg cgtatggcaa
    31861 ttccacaggc aaatccgagt ctttatttac caatggctct cgcgattaca ttcccgttta
    31921 acatcattgt tgggattccg ttgtattatt atttagtaac tcaattcatt ggaggttaaa
    31981 aatgaaaaca gcaaagaaag tagaaatcat tactggttct attgaattta aatatattat
    32041 caaagtgttt agtgaaaata aaattgctta tactgcgatt acaaatattt ccggacaagg
    32101 agcacacggt gaaaaatctt ccgatgaaat atttggaatt ttaaaaaact cgtatcttat
    32161 ctcaacttgc aatgagcagg aatgggataa aataaaagaa gatgtgagga gagtgattaa
    32221 aacatatggc ggtgtttgta tgctaaccga tgttgaggtg ttcgcatagt taattttttt
    32281 atagcttttg cagtgagttt tattggatcg attcccttag gtgcaattaa tctcactgcc
    32341 ttgcagattt atctccagag agatttaaaa tctgtggtat acttttcgtt tgccacatcg
    32401 gcaatcgaat tcgtttactc cttttttgcg atcaagggtg agggactaat ttctagtaac
    32461 tcatctcttg atctcttttt taattatata tctattgttg ttctttttgt tttaggagta
    32521 actagcttat tatctaagtc gaaagtgctg tcgaggaagt ctttagaaga agcggataat
    32581 ccaaatttgc ctcaccctta cattgcatca cttagaaaag gatttacgct tggattacta
    32641 aatccacttg ctattccttt ttggattgtg atgattggtg tattggaaaa gcaaaatcta
    32701 attcaaacaa aatcaaactt tgaagttttg agtcttatgc ttggaattgg acttggcgga
    32761 tggatttgtg ttatttgctt tgcatttatt tcaggaaaaa taatacaaaa aaaagagttt
    32821 agacttacag tcgtaaatca aatcataggt gtaatttttc ttggtttggg aatttaccaa
    32881 gtttataaat tatttaaata actcagttta ggatcattca ctaacaaaag cagcgattga
    32941 gtttatctga acccagagtt tttatgaggt catctcctaa cgtaatgctt atccatctat
    33001 tgctttatga ctcagtagta agaatctatc ccagaatatt gattttagtc ttttatttgt
    33061 atttctcata ctcttaaaat catcctttac tcactatttc ttaattttgt ttaagattag
    33121 tcaccaagtg ctggaaaaga tttaaaaact gcctataaaa tatatctcag aaagattcaa
    33181 ggaagtgctc tgtaaagcaa actatttgga gaattttttt ctaggttcgt ttcttcttct
    33241 attgcttcga tgagattttg atttagtgtg ttggttttgt tttgcaagaa tgtgtctttt
    33301 ggtagagggc ttactttttg ttccggtggc gcttgttcca gttgagtttt ctgtcagttt
    33361 ggagagagtt ttaaattcat cctctgtaat ttctcgatag tcgcctcctt gtattccttt
    33421 taagtgaata ttcatgattc taattctttc tagttttgta acgttatatc caaagtattc
    33481 acacatacga cgtatttggc gattgagtcc ttgcactaac gtaattttaa aagcaaacgt
    33541 gttttctttt tttacaatac attttttggt aacttgtcct aatattggaa ctccctttgt
    33601 catacccgaa ataaaactat cggtgattgg tttatctaca gtaacaagat attctttatc
    33661 gtgattattt tctgctctaa gtattttatt tacaatgtct ccattgtttg taagaagtaa
    33721 tagcccttgg gaatctttgt ctaagcgtcc aataggaaaa atacgctcag aat
//
LOCUS       DAOQOS010000054        33235 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_577_length_33235_cov_6.698192, whole genome shotgun sequence.
ACCESSION   DAOQOS010000054 DAOQOS010000000
VERSION     DAOQOS010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 33235)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33235)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33235
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_577_length_33235_cov_6.698192"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..391
                     /locus_tag="PLF66_13160"
     CDS             <1..391
                     /locus_tag="PLF66_13160"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HMZ65041.1"
                     /translation="KDNEEYHSLMYAVRGGNLEVVKLLVQNKANVNAAGLDGWTPILD
                     AANLGYPEIVKFLLKSGAYLNEANTYEETPLILAAKNAHTAVVKLLLKQGADATLADA
                     EGKTALQHADAAGHKEIVEILKKNLSK"
     gene            395..2215
                     /locus_tag="PLF66_13165"
     CDS             395..2215
                     /locus_tag="PLF66_13165"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HMZ65042.1"
                     /translation="MNLDLNFILEKSTSAIFFADSQNKIIYVNKSFCSIFKETEEQVI
                     GKTFSSLIQYPNPNDLNSFEYQLDKARNLQEEIKGEHVLQLKTGKLLVFEVTTHPYLD
                     PEKGLTLISFLDDITYFRDSELILIQTNKKLQDLQLAVNQSSIVSITDPYGIIIDLNE
                     NFSKISGYKREELIGKKHNVINSGYHSNYFFMDMWLTISAGNVWQGEVKNKRKDGEYY
                     WVQSHIIPFLDDKGNIIQYLSIRQDITVRKNAESLLAASEKKYRLLYERAPIGIALIG
                     PDLKIIECNDFILDLLGYEESEYIGEYSLNFVHPDFIEYKKIAIEKLFNKEISRFYIE
                     EKRITKNGSYIWVGVYSNAVTDSAGNVVYRLDFIIDIENRKKAEEDFLKLDEAKNAII
                     NIVAHDLRSPISGIISLVDLMISEEQNTSNMKYLEMLQTSSHHALNITRDILEMSELT
                     RTNTFLDKESTELNSFLNECIQFQELQAKEKSIDILFTSKVEKMYVDINRDKMKRAIT
                     NLISNSIKFTKESGKIELTLSIKDQRAFITIKDNGIGIPKSKQPHIFEKFSIARRTGT
                     KGEKSTGLGMFIVKEIIDKHGGRITFESEENKGTTFYIEL"
     gene            2212..2637
                     /locus_tag="PLF66_13170"
     CDS             2212..2637
                     /locus_tag="PLF66_13170"
                     /inference="COORDINATES: protein motif:HMM:NF020397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HMZ65043.1"
                     /translation="MKQKKDLTESNQIIEDILKPHTKEILSIVAELRDFFKEEFPSLI
                     EKGYPMWKGIGYSDSKSGYLCAIFPLEGEVKLGFEYGIILKDKDNVLKGDGKQLRYIV
                     ITNESEINRKQIKDFVKQSLALPVLKKDKLAMIDLIRKA"
     gene            complement(2620..3618)
                     /locus_tag="PLF66_13175"
     CDS             complement(2620..3618)
                     /locus_tag="PLF66_13175"
                     /inference="COORDINATES: protein motif:HMM:NF012575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HMZ65044.1"
                     /translation="MSALDHWHPVLKSGELKNKPISIILNEKQIVLFRDKKGLVAGLE
                     NACPHRRMALSEGFLKNGRITCIYHGMSFDRKGNGISPGTEDCKISAKYFDCIEFYGA
                     IWIKSAESFAPFPYFNIEGFRLIDICRYTIDAPLELVLDNFTEVEHTPDVHLFLGYDP
                     SRMKEVLVETTTEDDFVRIKNTGPQKKLPFIFNSILELNKNDLFVDDWTTYFSPIYSV
                     YDQYWIDPKTNQSRENRFRIYVFFNPISDKKVQLFAFSYMKYSLFGNFGLNLFIKPSI
                     KYIINLEILRDKRMLEKILDKDPHVAGMRLTRFDRSLGPNRKRIEKIYKGNSSLSN"
     gene            complement(3627..4763)
                     /locus_tag="PLF66_13180"
     CDS             complement(3627..4763)
                     /locus_tag="PLF66_13180"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF012946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HMZ65045.1"
                     /translation="MGELKEIHTLIIGGGPSGLAIGAVLRNGGVSFSIIEKYDKVGHA
                     WHNHYERVHLHTVKQLSSLPFMPYPSDYPRYVSRDLFTAYLDLYSKKFSLIPYFNQPA
                     KSIRYENGYWYTETPDVMYKTKKVVVATGYNHSPHSPKWDGMENFQGKILHSKEYLNG
                     KPFTGKRVLIVGIGNTGGEIAIDLFESGAKVDICVRGPVRIVPREIWGTPMQYSALLF
                     SYLPPKIGDYLSKTILGLILPDYSEYGLITPEYGTISQLKFQEKVPLIDVGTVSLIKQ
                     GFINVMPDIVRITKDSVVFKNDREEKYDVILLATGYRSGLDSILSNHPELLNEKGYPK
                     IRGQESSMKGLYFLGFNNHVGGFLRNISIEAKKIGSQIIKEFPN"
     gene            complement(4807..5580)
                     /locus_tag="PLF66_13185"
     CDS             complement(4807..5580)
                     /locus_tag="PLF66_13185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65046.1"
                     /translation="MKFLFLKSCFLFFIILPFLISAESEIKPTHVYTDCKFHPLKVKD
                     RLDCVVEDFSGNEMVLYVYEIQNQTLSKNPQKIIIPSWYNFATVEYIDILGEGKKMIF
                     AVFEGTAGSNTMQKILIVFHFQNDSFRPVLFETISYVLSRRGLSQSLNLKYKFLDFGT
                     KRVSIQLDYTYNVIISNKVQQDRRKTWTDELIWDNKNLTFYDAEQETKKNKKAEFHVE
                     KKIYETRLEFPPIHSINMEKITDLIYKSKIFDVLVDEKS"
     gene            5753..6658
                     /locus_tag="PLF66_13190"
     CDS             5753..6658
                     /locus_tag="PLF66_13190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65047.1"
                     /translation="MFRIKLLLLFIFTSSTLLANMRSPWVINRYPSYSLTKTNTELKV
                     LKEDLNFYCDRVYEGDPNYESLLDKKCKVEVAYTIQSNKDAKLYFEFILPSEKDVFVS
                     INNENENIAQTKAILVSDTEKEGYRLSDLCRFCEKTINSLYSASFSGKMTTGENIIKL
                     RYDQPLAVSEISYGYFKSSKWSNSFSYELWPLKEWKLDPNFKITIKFSTEVGGYFERF
                     YSEKISADCRGIDLRFSKIPSDPFKKEIGSNGFKEYYEYNQNLNPMYLLKDSKTSYEK
                     NRLVYELSFTDKFPDRLQCFFGKEK"
     gene            6655..7575
                     /locus_tag="PLF66_13195"
     CDS             6655..7575
                     /locus_tag="PLF66_13195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65048.1"
                     /translation="MNLTQKINFTFYSLLILLNLIHCKDAENPYIPTPDIWSRNYLEN
                     IPRNYEIETEVREILNTGAKFCETSFQNKKINLVGDNDNIFIEGVDQVNSFVYLSVYK
                     QFIKGKSDIFILKSRCCPMGPCYTSYFFQKQQNGEWKLFDTIAGEVIDYKKTKDSTIV
                     KILDSSLTGLTFMGFWKDEKFQPFLAYHQMNLEIPPTFSREIKKIPEARQLSLLKKPK
                     DYHVDNLSFRIQVKADTNVFILNETEKHYFVLVKASKEQVSDVVENFKAEREVLVSQI
                     QGIYPILEKTKDLEKLLDKIYYNIGWIEKF"
     gene            complement(7610..8542)
                     /locus_tag="PLF66_13200"
     CDS             complement(7610..8542)
                     /locus_tag="PLF66_13200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008593737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HMZ65049.1"
                     /translation="MKPIQTIDCHYIIDEFAAAYLMLEGEEALFIDNNTNFAIPHLLA
                     RLEENKIKRENVKYLIITHIHLDHAGATSRLVQELPNAIVLAHPKASPHVINPKRIIE
                     SASMVYGKENFIKMYGDILPVPENKVRSMQDGEVLKFGSRELTFIYTRGHANHHFVIY
                     DSKTNSIFTGDSFGISYPWLREGSSPFLFPSTTPTDFHAEEARISIEKIIKTGADTAY
                     LTHYDSWTDMKLGRDQMLFGIDRMEKIYLELVDANRDDSGLQELAVSRIHSYFEEEFS
                     KRNIPLSVIKKVNADIEINAQGLVFAAKRAKRKS"
     gene            9014..10918
                     /locus_tag="PLF66_13205"
     CDS             9014..10918
                     /locus_tag="PLF66_13205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004472507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HMZ65050.1"
                     /translation="MIKSINSLAIGGLFICFFTFGISSQEAKKTAKDTNPTSKQAKDG
                     KEAKETGKEAKDAESSPSFNLDPPAEVKYEEQPNDGKGIPEIQAELAKEEPYKSPMKG
                     KLSGDFLRSMLLSPEHQDAVRKVDRLWFGDIFRIGFQARPRLDYSFNRDFDKRTQDGT
                     NFGTQNSQVWFAIDPTPNVSAKVTIQDVRVWGGDQSRKDGQLGYLGLSNSAGIEVNST
                     PSSTNVINIQNRTGIREGFIVLKNYVEGLEFTIGRQVLGFGDNRYIGGRNDGQTGNSF
                     DGVKAKYAFNKMFSTEAFTSILAEESNGGGGNNTSNGQRKGTVNDTYLSGLYNTLKFE
                     DFLIDLYLFNIDRKWEQSITTTPPTSMDRTRQRDDLNTAGFRITNRTNNNILPKFKSW
                     DWTVESSMQFGNTGVRVNADWDVFQIQAPNGQRLYSQRQEYDSRFFLAQTGYTFFDRF
                     RVGIQYSVGTGDNSRSDSRMRTFDASFATRSGGFPYFDSGAGITNTTFWSNTRIKSLH
                     LMWNTEKLGRWIGVIYDTQKDQVNDAWYSSGGTPNTGLSYENTTGGRFGGSNTLGQKI
                     GKHLFYEYNLIWQYYLKDYVSIWAGASYLTAGDSIRGVRVNPLASDATARYSLDNKSY
                     SFFLFVQFAM"
     gene            10930..11859
                     /locus_tag="PLF66_13210"
     CDS             10930..11859
                     /locus_tag="PLF66_13210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65051.1"
                     /translation="MNDSKSKKYSRQILFLILILALFGFTKCTNELRNSGLGPKKKNN
                     NVQLQFLSYVYADTLTNLGVNSRCARSSGTIISNPAVANPNSTVYNLRNCNINKVDGF
                     STNRVTSSTTGFVGTSTSSLIVSNGSQVGTDGTGGKRNAEVTFTVNSSSGYLDVIVYG
                     DGNTNSFSGPTWRITSASNQFKDQNGSSSNSQIAYRSQDGVFRVPISGATPSLPANPI
                     RTVSTGSSRTICLDFFGSSNTLQAAWDGSCTSVATSQKSTRSSYPILIDPADANLTSS
                     SFAPQTTVGTKIGFILNNITLTSFTIANQLAQE"
     gene            11876..12880
                     /locus_tag="PLF66_13215"
     CDS             11876..12880
                     /locus_tag="PLF66_13215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008589795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HMZ65052.1"
                     /translation="MINFIRLISIATILTSGMTLLSKDKDITLLNVSYDPTREFYVEY
                     NKLFASYWNSKTGDNVRINQSHGGGGKQARAVIDGLEADVVTLAVSYDIDVVASKGLL
                     APTWQKSLPNNSTPYTSTIVFLVRKGNPKSIKNWDDLIKPKVSVITPNPKTSGGARWN
                     YLAAWGFAAKKFNDDRGKIEDFIKTLYKNVPVLDSGARGSSTTFAQNGIGDVLLTWEN
                     EAFLIQNELGKDKFEIVYPSLSILAEPPVAVVEKNTEKHGTTEVARAYLKSLYSEPAQ
                     ELIAKFGYRPRSEAVLKKYESKFPKIELLTIDGNFGGWTKAQKEHFADGGTFDKLYVK
                     "
     gene            complement(13074..14255)
                     /locus_tag="PLF66_13220"
     CDS             complement(13074..14255)
                     /locus_tag="PLF66_13220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HMZ65053.1"
                     /translation="MKQIESAPFKEMAPTTPVETNSIIKKTYGRYLDEFKDGDVFVHP
                     RAFTIDFSFAQEFATTFMEANPLFLSRPYAQAHGFKDMLVSPLMVFNMALSIGVQNDS
                     EKAMANLGYYNVQFLAPVYPGDTLSARTKVLKVDDKGPDKPGIAHVRTLCLNQSGDLV
                     LQYERKIMINHSNGKPKGDSKPGNPNSHFPETDKPVIKLPSLLYPKDFRETTWESTYF
                     ESFKAGQIYIHANGRTITDEHCAWTYRVGNTHPLHYDQLYSKSLTGAMSGERIVYGGL
                     VFAWLVGMASRDISENMLWDLGYTEGYHTQPSISGDTVTAISRIISAEDVGDKYGIPA
                     GEVHLQFIGLKNIKADAAYEKYGSDLFIKENDKKKLGKEKLPEKIFEIERKLVIKKKP
                     V"
     gene            complement(14341..16413)
                     /locus_tag="PLF66_13225"
     CDS             complement(14341..16413)
                     /locus_tag="PLF66_13225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65054.1"
                     /translation="MGSILNTISLLLSQSIYLISFYLGCVILFYSEKKDRQAIVAGIS
                     FFLCSLFFIGHLLVLGKGFIYILSLSSSIIQPTIIILSLLPLAWTYIVYRFYDDIFLF
                     GKFSFRNTVFLILAIYFLITTFIVIYFIPIQKNRTVYDIILQIPDTVFYIYSCYIILC
                     TLSSILFLLRTELKSNSLEEIAKSSSYRTILNSSYILFFLSILVGIASIQGRDYLNVE
                     ISSIRSNPFPSFLLFLDSIANIAIMATIVIVGRAVISYQVFTGRYLAMKSLKEYWKNI
                     SIFVFANGFILVCSGLLGYSTDLKQVLINFLAIFSIVQVQMISRKREEENKKTLKPFL
                     FNENLYLSITDTSQRIEFQLEKTFQLLCDTTLNTTRACLIPLGAYSSYIPKPLFYPEE
                     KLDFKINNFLSQVPQNKSLFYLNREKFSGYVLGITIQDKNENIGVLYLGVKKDYTLYT
                     EEEIDIARIGSGKIMDLLSVAEISRLLVNLQKKQLMDSKILDHQTRRVLHDDILPEIH
                     SIMIEIDSMTNELAKKNLINSLSDLHKKISNLLKLIPLHPTNLNHTNFIIEIENLVKS
                     ELGGESVQYQIQEEAEKKLELLESIALEVIYYAVRELLRNISRYARSPDRALEVQIYL
                     ILENGNLELWIEDNGKGYEESKEQKGAGQGLLLHSTLMAVIGGALIKETHIGESTKVR
                     LILKKFFL"
     gene            complement(16398..17144)
                     /locus_tag="PLF66_13230"
     CDS             complement(16398..17144)
                     /locus_tag="PLF66_13230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003006438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HMZ65055.1"
                     /translation="MLEQLKIILIEDNVKLRKALKQGLEETKKVSVLYDCDNGAAALE
                     FCRSHEVDVLLFDVQIIGPNGIESAITIRKEFPRKPVVFYSIQDDDSYFRDFRRSGIL
                     SHYAYVKKSNYLLPSMILPLLKDAYSGRSFIDPEIETRVAEVKYSDENSPLALLEPNE
                     REVAKLISVGLSNEQIALKLGVKDKRTISRINGQIYTVWGLADNATDEKVARTRASLI
                     VRENKLLTWTEKGKIFYLNERGEEVNWDQY"
     gene            17264..18562
                     /locus_tag="PLF66_13235"
     CDS             17264..18562
                     /locus_tag="PLF66_13235"
                     /inference="COORDINATES: protein motif:HMM:NF014453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HMZ65056.1"
                     /translation="MRELITITFTILALGLFSPLMILIAVCIYLEDKGSIFFLQERIG
                     LREIPFMVIKFRTMRDGKVTYVGNILRKTGLDELAQLFNILIGDMNIVGPRPLTRYDI
                     DRLGWNQYKFTKRFSVKPGLTGLAQIYGGRGARISKVFEFSYLKLSSLSLDLRIIFVT
                     FFMNAFGKKKIRNYLWKKLKSRRRTPSWKNWLTYFKKNSLSPMKESIDTVNLSERKLE
                     ALRYSLSIFQLGESGEGRIAKEIDKCNIYGVNETYRECLKLFVKEEGKHGRLLALMVR
                     SLGGKLAKHNWTESLFSFGRRLMGIRLKLLVLLAAEAVSVVFYKLFIKELPLSGMRAT
                     LIEICRDEEFHLRFHQDFFKIRMNTKFSKIIFKLLWRSITLTAFCVVVLDHRKSLLVF
                     NASITKIFEEYKSIVLKVEEEVCFKNNLSKPFSKLVLHLR"
     gene            complement(18555..20186)
                     /locus_tag="PLF66_13240"
     CDS             complement(18555..20186)
                     /locus_tag="PLF66_13240"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HMZ65057.1"
                     /translation="MELTLRKKLIIGFTLLDLIISILLGVFMFQFSYGMFFQNFKDHK
                     TSIAKFGSSLLSGEEDPLLQSEKQSNQSDLKEKIKILNQIIFSENNIQSICILSYSPD
                     EDNFRYVIKNDGTMNGQVYQAEKSQIEKLRKVLREKSREPVFSEDEIFTKEAYVYSKI
                     FNLKEEVIGILVLEIDSKPILDFKTKMAIVAIVLCIFTFCLTGLVAVFMAKYFFSPLE
                     VLSDAVNNLASGNLIMIENFDKKDEFGKLAKSFNAMVNNLKIASEVQYNLIIEISNLN
                     DNLEKIVEERTRTIQSQSQELEKQIMIAKKIQLSLLPEDVPRIKGATLSFRYQPMMGV
                     GGDFIDFDYKNTNDLILFICDVSGHGVPAAFLATMVKMSLQECYDMKLSPAESIQRIQ
                     RSLQGKLSGHFLSAVYCHIDLQKGLMSSANAGHLPILKVGLNEEHQFINSKGRIICEA
                     FKPSSEQIITKLEVGDKIILYTDGVTEARNNKNELFGYERLVDISLKHHFETSFNMCN
                     HIYNSVLSFMGNSDAHFTDDITLLVAEYSGKEISI"
     gene            complement(20342..22033)
                     /locus_tag="PLF66_13245"
     CDS             complement(20342..22033)
                     /locus_tag="PLF66_13245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65058.1"
                     /translation="MKTFLIALYLLFEISLSAIEPEKIDSVYWPMQVRLPISGTFAEF
                     RNSHLHMGCDYKTYGINGFSVLSVFDGNIASMSYSANGYGLSLNLFSPSLKIYAKYGH
                     LNDLSGDVYGLEELKNALLLLGKPEGFQVKLKPDFFKVKSKDRIARSGETGSGVSHLH
                     LELFDGKEYFNPLSFINYKQEDKNPPVIQSLFIDSDNGFSKNFPAISITKNTYSFTNP
                     EEVKLSGKIKLKVAGFDRISSKNQNNVYAVKLKINELVVFERNFNRMTYKEASDKDSL
                     YDMNKSSLSPAFYVYNLYDDKNSHSIDLNTVDEGKQMLAEVLLLDASGNESSLKFKFM
                     TVKNENYVKTKPATKFSSEDEKLTLNFSKTSIIGDGGIKITRLDKIPSEVNLTNLIPS
                     GDAYQITSYNFSWKGGADGVLSGNFPDKEEALYLYDTVLNRWVPIAGKKSTNSISFSF
                     HRLGIISVMRDKSPPVINYPYLIYRHYNLPEIQDSTMIERFYHASDKGSGVSGRIEVL
                     LEGSSYPYTFDRDRNFIKLEIPKTIRSIKNPVLVQVRVSDNAGNKSNWFTDIIRF"
     gene            22082..22681
                     /gene="lptE"
                     /locus_tag="PLF66_13250"
     CDS             22082..22681
                     /gene="lptE"
                     /locus_tag="PLF66_13250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012389288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HMZ65059.1"
                     /translation="MGLSFISFRNFTILFIFSFHFFSCAYFLKEPGRPPKVNGVLIDD
                     SYRRLYIHNIQNDSYGNAVHTMLTPMVKAEVDRRGRFIQTRDKSSASFRFYASVIHYQ
                     KVGNLMDAFGQHISAEISLVVRLEIQEAATGEKIPLERDEILIRGYYSDQMGYRESEE
                     QAQSRMLQKLAYRISEESENAWYYFVKEKYYKNVSLEKK"
     gene            22722..23027
                     /locus_tag="PLF66_13255"
     CDS             22722..23027
                     /locus_tag="PLF66_13255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65060.1"
                     /translation="MKKIKLSRGKSALIDDDDFERVSQYNWYINENAEKTKLYAMRSK
                     LKSEEGIVKGTKVYMHRFILGIVEKSAVIQHKNGNTLDNRKANLVVKKRTVTKKSAK"
     gene            complement(23030..24475)
                     /locus_tag="PLF66_13260"
     CDS             complement(23030..24475)
                     /locus_tag="PLF66_13260"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HMZ65061.1"
                     /translation="MKFTYLLFITILLFCKFENQEEYSNIVRVGIASDPTTLDPIYSV
                     DLTSQKINSLLFVKLFHYKKDGSITGDLANEFKLNGNKILIQLKNEKTIKGYTLNAND
                     VKYSLNRLRTEKGPRKSRYSFIQDIKILSPFQIEITFDQANRKYLDLLSLAPASIYRE
                     ESHKINGDFISSGIYFLEKWNKNETIELSLNRADTVGYPQRLFLQVLNQPSSAIYLFQ
                     KSQLDIFKVPYFLLEHPAIANKERKSIKGKSVQYIAINHTNSCYDLAFRKALNYAVDR
                     NQIIEKIFNSSASDVNTTVTNEYLEPFTNDKFYYTYNKDLARKYLEESKCYPDILTKK
                     IELRMRADDENKAKGSVLAQYLKDIGLNIIILPMEKTKLYKENGEKKGDLTLLTWYID
                     YDSVYNFIDPILASDAFGNGGNRSFYANRTVDEFIKQTRNNPDAGINPKDILNIIKED
                     APLVFLWSIHENYILSNQAKQFPELIDVILQ"
     gene            complement(24472..25278)
                     /locus_tag="PLF66_13265"
     CDS             complement(24472..25278)
                     /locus_tag="PLF66_13265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65062.1"
                     /translation="MSLFPQDSESEKSIVETETNTKKNQDEKRLSAQFIIGNGVGQLG
                     VLKDRYDPATSLSQFVVLALFSSAQAAPGFASSLSGNLNSSSLFNLYVLNDQLNSAAI
                     QSSSQSSINRFYVEAHPPDDHIAFLYGLSTGSYSLKADASRNNNVFLPSLFFGSSNPI
                     LNIYMAQSFVENSRAINLSIDTLDGAVKYYFTPESKFDPYVSAGLGVGSCFFNCVAIR
                     FFGKVGFRYNFESSYLFLEEEFQYVYFHTKENIYNPMRERMTLLGFGVYF"
     gene            complement(25361..28207)
                     /locus_tag="PLF66_13270"
     CDS             complement(25361..28207)
                     /locus_tag="PLF66_13270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HMZ65063.1"
                     /translation="MESLVQFTLDFEKIPTKEVKGEVVYLKSHPEFGFGKIISATKTG
                     LTVYFLSVGKELLIDHSDPNLQYVSSNLKALYTKEERKFEMNLSLKALELKLTHAFDK
                     LSSLSNSRTRLLPHQIESTYIVVNSLKPRFILADEVGLGKTIEAALIMKELIFRNAYK
                     RVLIVTPSPLLVQWKQELKNKFNEDFTIIKRKNFISDDENNWQNFNHVITSVDFIKNP
                     RYAEDILKKKWDIVIFDEAHRLRRDYSKITRAYVFAEKVSKKCECLLLLTATPFRGKL
                     EELFYLMHLVDPNILGPYHTFINDYVVGSKADLKEKISKVLLRRRKVEIGGFTKRFAK
                     TVKIDLSDAERQFYEETTEYVRREYNLAMNTKNRAIGFVMIVFQKLLDSSHVALLSAL
                     TRRKFMIESRMHKFKLKAEELEDWDLDETEGVEDFISGLDDSAHIELQNVRRELLSLN
                     RLILLGKSIKEDRKSMKLKETLYRLRKEGHKKFIIFTQFRSTQDYLYSILDDFKVVLF
                     HGSLSADGKEEAIQKFKEDFEILICTEAGGEGRNLQFSNVLFNYDLPWSPLKIEQRIG
                     RIHRFGQKKDVYIFNFASKDTVAERILEVLTNKIKLFEDSIGASDALLGTIEDELDFN
                     SSFMRFITGSKTKEEIEIEMDLRIKVAEQGFNKLNDLVTPKLIDFNLQDYYNHTLSER
                     QYNNTHLEEFLISFAKYFPNEFGSNLQINPENNRQYLIQNGKEVKHGTFFSEDALVND
                     SLEFLAFGHPLVEKSIQYLIDTKSGFARTYFSYPEYESKIFFVFLVEFQFSLKRTEIF
                     YLEYDSSIDMVSELNTIPEFIQDNVLAANINLSENPLRVESGFIRCYEKLLNIVETRK
                     DELVKNTLSIFKKEEFKIEVTSQKVIRQLQEKLNRQEAQEKWDPRPERKSVINRTKNE
                     IMKVREDYEREIRKVRNGSTIHSRIELFQVYIGC"
     gene            complement(28207..28917)
                     /locus_tag="PLF66_13275"
     CDS             complement(28207..28917)
                     /locus_tag="PLF66_13275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000369363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HMZ65064.1"
                     /translation="MNHVNAIVNEIFYSLSGEGVSQGVPTVFVRFAGCGLRCGMDGSR
                     KLWCDTGYALSHNAGTSMSLTEILEKVESFSSTPTQILLTGGEPLEGDRKFFCSNFAK
                     IVYEKRHNSKFAFTRVETNGKEDIQNLQNMVFSIDYKLPGSGMEDQMNPDNFHFIRER
                     NNSLDEVKFVVRDRKDFDRSLEVIQEFQLNQNLIFSSVYTELKPQLLADWLKDANIEG
                     ARFSIQLHKYLWGEVRGV"
     gene            complement(29399..30070)
                     /locus_tag="PLF66_13280"
     CDS             complement(29399..30070)
                     /locus_tag="PLF66_13280"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ElyC/SanA/YdcF family protein"
                     /protein_id="HMZ65065.1"
                     /translation="MKNFYFKIAVICISFLILSFLFALYLSYSIHKNAESFLFRDLNK
                     IPNCYTGLVLGAFVKKTGIPSDVLEDRLLTAVTLYRNKKIKRFLLSGDHGRKNYDEVN
                     NMKAYLKNLGIPEEDIFLDHAGFDTYDSIVRAKEIFAVQDVILVSQEFHLPRAIYIAR
                     SKGLSAYGAISENYQYNTFQNLKNSFRDIFACVKAYLEVSLNLKPRFLGEKIPITGDS
                     KLSYD"
     gene            30344..31762
                     /locus_tag="PLF66_13285"
     CDS             30344..31762
                     /locus_tag="PLF66_13285"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HMZ65066.1"
                     /translation="MNQKTTGFEKCEVDLSNTGFIQGFGCLLRVSYKTRSIVSVSKNS
                     SEFLQKESLIGKTLTDVFGEDMESRLWNYPFKAPINESWENFKIYGQSDEFGIILEIE
                     ANTDLPKRKPRFVLFGSKTREEIINKTLTRLKEESGFERVMLYEFLPDGSGQVTAELY
                     SGSGDNYLGLRYPATDIPAVARKLYLRNAYRYIYDATQESIPILNANNEIDTTVNLSY
                     CFLRNVSPFHKEYLKNMGVKCALSFSLVREDRLIGMFSLHNIEPKFVSHESRLQMVQI
                     TKEFLHNLSIRESEERMQFLDGYRREVTNFIQSVNHGHERENEILGFLQLVGATGLAI
                     KMGENWTAYGKVQITEELKSIEESITQVEAKGIFTTDCISSIYIGMESDKDFASGVLC
                     VWYTDLSTKEKKSFLFVKPEVLQEVVWGSRVELYPGDVNPLNSFGQWKELMSMHSNPW
                     SRQSHNAAQAILYVGLSKSFPI"
     gene            31986..33230
                     /locus_tag="PLF66_13290"
     CDS             31986..33230
                     /locus_tag="PLF66_13290"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HMZ65067.1"
                     /translation="MKRKRILLAEDDQYTIKYLSDLISNHGYHIDAARDGLEALNYYK
                     QDNYPVILTDLNMPKLNGLDLVKEIRSLNEISVIIVLSNETDIERVISVMREGVYDYL
                     IKPPRPEDILIKINHAYKLSQLNLMKFNIEKERELRLNNQLNWMNWVKTKQSSQKETY
                     ETVLQNLKHSLNQGAGIGTIVSLIDILKSNGKLENDKYIFPKKIIELLYENGKFASNT
                     LERVELIAGLGEREIHLEPVSVNDLIDFLNKIILAQTELLTIKQQRIVLSNTSNTKSE
                     RFVRLDQNLFQLLISEILVNAMKFSEENTAIYVLFFIGVNLTITVLSPPNSNANTIGV
                     PPEMESLCLEPFFRVSNVIDERYRTLEMGLGLTMAHKIMKIHSGNLEITNIKNYLEDR
                     DVFPVKVQTTLTFPLDTQLKFS"
BASE COUNT        11584 a         5651 c         5290 g        10710 t
ORIGIN      
        1 gaaagacaat gaggaatacc actctttgat gtatgcagta agaggtggaa atttagaagt
       61 tgtaaagctt ttagtccaaa ataaagccaa cgtaaatgca gctggattag atggatggac
      121 tcctatttta gatgcagcta atctgggtta tcccgagatt gttaaatttt tgttaaaatc
      181 aggcgcctat ttaaatgaag caaatacgta tgaagaaaca cctttaattt tggcagcaaa
      241 gaatgcacac actgctgtag ttaagttatt attaaaacaa ggagctgatg ctacacttgc
      301 tgatgcagaa ggaaagacgg cactgcaaca tgctgatgct gctggtcata aagaaattgt
      361 agaaatatta aaaaagaact tatcgaaata aaatatgaat ctagatctta attttatttt
      421 ggagaaatca acttctgcaa ttttcttcgc tgatagccaa aataaaatca tctatgtaaa
      481 taaatctttt tgttcaattt ttaaagaaac tgaagaacaa gtcataggaa aaacttttag
      541 ctcactaatt caatacccaa acccaaatga tctcaactct tttgaatacc aactagataa
      601 agctcgaaat ctacaagaag agataaaagg tgaacatgta ttacaattaa aaactggcaa
      661 gctattggtt tttgaagtaa ctacacatcc ctatttagat ccagaaaagg gtttaaccct
      721 aatcagcttt ctagatgaca ttacttactt cagagattct gaattgatac tgattcaaac
      781 gaataaaaaa ttacaagact tacaattagc ggtaaaccaa tctagtattg tatccattac
      841 cgatccttat ggaatcatta tcgatttaaa tgaaaatttc tctaaaatca gtggatataa
      901 aagagaagaa ctcattggta aaaagcataa cgttataaat tcaggatatc attccaatta
      961 cttctttatg gatatgtggc tcactatttc agctgggaat gtttggcagg gtgaagtcaa
     1021 aaataaacgc aaagatggag aatattattg ggttcaatcg catatcattc cattcttaga
     1081 tgacaaaggt aatatcattc agtatctatc gattcgccaa gatataacgg ttagaaagaa
     1141 cgcagaatcc ctattagccg caagtgaaaa aaaatataga cttttatatg agagagctcc
     1201 catcggtatt gctctgatag gtcctgactt aaaaataatt gaatgtaatg attttatttt
     1261 agatttactc ggatacgaag agtcggaata tattggtgaa tattctctta attttgttca
     1321 tccagacttt atcgaatata aaaaaatagc tattgaaaaa ttatttaata aagaaatcag
     1381 tcgattttat attgaagaaa agagaattac taaaaatgga tcgtatatct gggtaggcgt
     1441 ttactccaat gctgtaactg attcagctgg aaatgtagtc tatcgtcttg attttataat
     1501 agacattgaa aatagaaaaa aagctgagga agatttttta aaattggatg aagcgaaaaa
     1561 tgctattata aacatagtag cacacgatct aagatctcca atcagtggaa ttattagctt
     1621 agtagattta atgataagcg aagagcaaaa tacctcaaat atgaaatatc tagagatgct
     1681 ccaaacttct agccatcatg ccctaaatat cactcgcgat attttagaaa tgagcgaact
     1741 cacaagaacg aatacttttt tagacaaaga atctacagaa ttaaattctt ttttaaatga
     1801 gtgtattcag tttcaagagt tacaagccaa agagaaatca attgatattt tattcacaag
     1861 taaagtggag aaaatgtatg tagatatcaa tcgagataaa atgaagcgag ctataacaaa
     1921 tctgatttcc aactcaatta agtttacaaa ggaatcgggt aaaatagaat tgacattatc
     1981 tataaaagac caaagagcat ttatcacaat caaagataac ggaatcggaa ttccaaaaag
     2041 taaacagcct catatttttg aaaagttttc tattgcaaga cgaacaggaa caaaagggga
     2101 aaagtcaaca ggacttggga tgtttatagt aaaggaaatt atagacaaac atggtggacg
     2161 tataacgttc gaaagtgaag aaaacaaagg aacgactttt tatatagaat tatgaagcaa
     2221 aaaaaagatc taactgaatc caaccaaatc atagaagata ttttaaaacc tcatacgaaa
     2281 gaaatactat caatcgtagc agagttgaga gattttttca aagaggaatt tccatctcta
     2341 attgaaaaag gctatcctat gtggaaagga ataggctata gcgattcaaa atcaggttat
     2401 ctctgtgcca tttttccatt agaaggcgaa gtaaagttag gttttgaata cggaatcatt
     2461 ttaaaagaca aagacaatgt tttaaaaggc gatggaaaac aactacgcta tatagtgatt
     2521 acaaacgaat cagaaatcaa tcgaaaacaa atcaaagact ttgttaaaca atcgctagcc
     2581 ttaccagtct taaaaaaaga taaattggcg atgatcgatt taattcgaaa ggcttgaatt
     2641 tcctttatag attttttcga tcctttttct atttggtccg agagagcggt caaaccgtgt
     2701 tagccgcatt ccagccacat gtggatcttt atctaaaatt ttttcaagca ttcttttatc
     2761 tctcagaatt tccaaattta taatgtattt gattgaaggt tttataaata gattcaatcc
     2821 aaagttacca aagagagaat atttcatata ggaaaatgca aagagttgca ccttcttatc
     2881 cgatattgga ttaaaaaaaa catagattct aaatctgttt tccctgcttt gatttgtctt
     2941 tggatcaatc caatactggt cgtaaacaga atagataggg gaaaaataag ttgtccagtc
     3001 atctacaaac agatcatttt tatttaattc caaaatagaa ttaaagataa aaggaagttt
     3061 tttttgaggt ccagtatttt tgattctaac aaaatcatct tcagttgtag tttcgacaag
     3121 cacctctttc attctgcttg gatcatagcc taaaaataaa tgaacatcag gagtgtgctc
     3181 aacctctgta aaattatcta agaccaattc aagaggagca tctatcgtat agcggcatat
     3241 atcaattagt cgaaaccctt caatattaaa atatgggaaa ggagcaaaag actctgctga
     3301 tttgatccag attgctccat aaaactcaat acagtcaaag tattttgcag aaatcttaca
     3361 atcttccgtt ccaggactga tcccattacc cttgcgatca aaactcatac cgtggtaaat
     3421 gcatgttatc ctcccattct ttaaaaagcc ttcactaaga gccattcttc tatggggaca
     3481 agcattttct aatcctgcta ctaaaccttt tttatctctg aacaatacaa tttgtttttc
     3541 atttaggatg attgagatag gtttgttttt caattcaccc gatttcaaaa ccggatgcca
     3601 atgatccaat gcagacatgt ttcctcctag tttggaaatt cttttatgat ttgacttcca
     3661 atcttttttg cttcgattga gatatttcgc aaaaaaccgc ctacatgatt attgaaacca
     3721 agaaaatata gacctttcat ggaagactcc tgtcctctta ttttgggata acccttttca
     3781 tttaataatt ctggatgatt ggataaaata gaatccaagc cagatctata tccagtggca
     3841 aggagtataa cgtcatactt ctcttctctg tcatttttaa acacaacaga atcttttgta
     3901 attcttacta tatcaggcat aacgtttata aatccctgct taataagact cacagttcct
     3961 acatcaatga gcggaacttt ttcttgaaat ttcaactgtg atattgtccc atattcaggt
     4021 gtaataagtc cgtattcaga atagtctgga aggattaaac ctagaatggt tttactcaaa
     4081 taatcaccta tcttaggagg aagataacta aaaagcaaag ctgagtattg cattggagtt
     4141 ccccaaattt ctctcggaac aattctgacc ggtcctctaa cgcaaatatc tactttagct
     4201 ccggattcaa ataaatcaat ggcaatttct ccgccagtat taccgatacc tacgattaat
     4261 acgcgtttgc cagtaaaagg ctttccattt aggtattctt tgctgtgtag aatttttcct
     4321 tggaaatttt ccataccatc ccattttggg gaatgcggtg aatgattata accagtggca
     4381 acaactacct tcttagtctt atacataacg tctggtgttt ccgtatacca atagccattt
     4441 tcatatcgaa tcgatttagc cggttggtta aaataaggaa ttaacgaaaa ttttttagaa
     4501 tataaatcaa ggtaggcagt aaataaatct cttgatacat accgaggata atcagaagga
     4561 tagggcataa atggaagaga cgatagttgc tttacagtat gtaaatgaac tctctcataa
     4621 tggttatgcc atgcatgacc aactttgtca tatttctcta tgatgctaaa ggatacaccc
     4681 ccatttctaa gaactgctcc aatcgctaaa ccagaaggtc ctccgccaat aattaatgta
     4741 tgtatctctt ttaattcacc catgaatcaa aacgtctgac tcaagggtat tcttagcaag
     4801 acttttttat gatttctcat ccactaagac atcgaaaatt ttagatttat aaatcagatc
     4861 tgttattttt tccatattga tggaatgaat aggtggaaat tccagccttg tctcatagat
     4921 ttttttctct acatgaaatt cagctttttt gttctttttg gtttcctgtt ctgcatcata
     4981 aaaagttaag tttttattat cccaaatcaa ttcatctgtc caagtctttc tgcgatcttg
     5041 ctgcacctta ttcgagatga ttacattgta agtataatcc agctgaatcg aaactctttt
     5101 tgttccaaaa tctaaaaact tgtattttaa attaagcgat tgcgatagtc ctctacgtga
     5161 caaaacataa gaaatcgttt caaacaacac aggtcgaaaa gaatcatttt gaaaatgaaa
     5221 tacaatgaga attttctgca ttgtgtttga tcctgcagtt ccttcaaaaa cagcaaagat
     5281 catcttcttc ccctctccta atatatcaat atactcaacg gttgcaaaat tataccagga
     5341 gggaataatt atcttttgtg gatttttaga aagagtttga ttttgtattt catatacata
     5401 aagcaccatc tcatttcctg aaaagtcttc tacaacacaa tcaagtctat ctttaacttt
     5461 caaaggatgg aatttacaat cagtgtaaac atgtgttggt tttatttctg attctgccga
     5521 aattaaaaaa ggaagtatga taaaaaaaag aaagcacgat tttaaaaata gaaacttcat
     5581 ttttatacaa gcctccttct ttttatacta tcgaattttt tataaaaata ctaatactaa
     5641 aataaagtat ttaaactgaa tggaatacta aaatagcatt gcagtttttt tattctataa
     5701 attgattaaa gactttgtgc aactctgcaa aactgcatac ataagaggaa atatgtttcg
     5761 tataaaacta ttattattat ttatttttac cagtagcact ctactcgcca atatgagatc
     5821 accatgggtc ataaaccgat acccatcgta ttcactaact aaaacaaata cagaactaaa
     5881 agtattaaag gaagatttaa atttttactg tgatagagtt tatgagggag atccaaacta
     5941 tgaatctcta ttagataaaa aatgcaaagt agaagtggct tatacgattc aatcgaataa
     6001 agatgcaaag ttatattttg aatttatatt accctccgaa aaagatgtat ttgtttccat
     6061 taacaatgaa aatgaaaata ttgcccaaac gaaagcaatt ctagtttctg atacagaaaa
     6121 agaaggctac agactatccg atttatgtag gttttgtgag aagacaatca attcactcta
     6181 tagtgcttct ttctcaggaa agatgactac aggagaaaat ataattaagc ttcgttacga
     6241 tcagccgtta gccgtcagcg aaataagtta tggctatttt aaaagtagta aatggagtaa
     6301 ttccttttca tatgaactct ggccattaaa ggaatggaaa ctagatccaa attttaaaat
     6361 tacaattaag ttttctacag aggttggcgg atattttgaa agattctact cagaaaaaat
     6421 ctcagctgat tgtagaggaa ttgatctacg cttctcaaag attccaagtg atccttttaa
     6481 aaaagaaatt ggttcaaatg gttttaaaga atactatgaa tacaatcaaa atttaaatcc
     6541 aatgtatcta ctaaaagact ctaaaacttc ctacgaaaaa aatagactgg tatatgagtt
     6601 atcttttaca gataaattcc cagatcgttt gcaatgcttt tttggaaaag agaaatgaat
     6661 ctaactcaaa aaataaattt tacattttac tcactgctaa tacttctaaa tttaattcac
     6721 tgcaaagatg cggaaaatcc ctatatacct acacctgata tttggagccg aaattactta
     6781 gaaaatattc cacgtaacta tgaaatagaa acagaggtta gggaaatttt aaatacagga
     6841 gcaaaattct gcgaaacttc cttccagaat aaaaagatca accttgttgg cgacaatgac
     6901 aatatattta ttgagggagt cgaccaagta aattccttcg tttatctttc tgtttataaa
     6961 caatttatca aaggaaagtc agatatattt attttaaaat caagatgttg tccaatggga
     7021 ccttgttata cttcttattt ttttcaaaag caacaaaatg gagaatggaa attatttgat
     7081 acgatagccg gcgaagtgat cgattataaa aaaacaaaag atagcacaat tgtaaaaatt
     7141 ttagacagtt ctctaacagg tttaactttt atggggtttt ggaaggatga aaaatttcag
     7201 ccttttctag cctatcacca aatgaaccta gaaatcccac ccaccttttc gagggaaatt
     7261 aaaaagattc cggaagcgag acaactcagt ttattaaaaa aacctaaaga ttatcatgtt
     7321 gataatctct cctttcgtat tcaagtaaaa gcagatacga atgtttttat tttaaatgaa
     7381 acagaaaaac attattttgt cttagttaaa gcaagcaagg aacaggtttc tgatgttgta
     7441 gaaaatttta aagctgagag agaagtttta gtttcgcaaa tacaaggaat ttatccaata
     7501 ttagaaaaaa caaaagacct agaaaaatta ctcgataaaa tctactacaa cataggttgg
     7561 attgaaaaat tctaataact aagtaaaatc atattcttta ttacaaagac taagattttc
     7621 gcttcgctct tttggctgca aatacaagac cttgtgcgtt aatctcaatg tctgcattca
     7681 cttttttaat caccgaaaga ggaatatttc ttttggagaa ttcctcttcg aaataagaat
     7741 gaattctact gacggctaat tcctgtaaac cgctatcatc tctattcgca tcgactaact
     7801 ctaaatagat tttttccatt ctgtcgatac cgaaaagcat ttgatctctg cctagtttca
     7861 tatcagtcca tgaatcataa tgagtcaaat aagcagtatc cgcacctgtt tttataattt
     7921 tttctatgct aattctagct tcctcggcat gaaagtcagt aggtgtagtg gacggaaaca
     7981 aaaaaggcga acttccttct ctcagccatg gatagctaat tccaaaagag tcacctgtga
     8041 atatagaatt cgttttagag tcataaatta caaaatgatg atttgcatga cccctagtat
     8101 agataaacgt taactcccta gaaccaaact ttagaacctc cccatcttgc atgcttctta
     8161 ctttattttc tggaacaggt aagatgtcac catacatctt tataaaattt tcctttccat
     8221 agaccatact tgcactttca ataattcgct ttggattaat cacatgagga ctagcttttg
     8281 gatgtgcgag aactatcgca tttggaagct cttgcaccaa acgagatgta gcccctgcat
     8341 ggtctaaatg aatatgagta atgattaaat acttaacatt ttcccttttg attttatttt
     8401 cttctaatcg agcgagcaaa tgcggaattg caaaatttgt attattatca ataaagagtg
     8461 cttcttctcc ttctagcatc aaataagcag ctgcgaattc atctataata taatggcaat
     8521 caatggtttg gataggtttc atgactcgat tcattcgttc aaaatagctt ttgtcaagat
     8581 gttttacttc tctcacattt tgtcttagag tgaattaata accgattggg aataaaaaag
     8641 aaagatttcg attttgagat tgagtattca ctgcttctac catcacctcc tcccgttctt
     8701 tacacagaga ataaacccgt aattctatct agattttaaa aaggaatgac gaggtatgaa
     8761 tcaagatatt caattctatt taaagaagaa ccagaaatct taactgtcca aagcaatatt
     8821 taagtgaaaa aagagaacat tatccaatat aacagaaaat agaactcatt ttctgccaat
     8881 ataattgatt ttatatataa tatatcatat tttttccgtt ttattgatta aattatccat
     8941 tatattggat attttgttgc aaaaatcacc ggcctatttc atataaacat attccaaaaa
     9001 aaggaggaat aaaatgatca agagtatcaa tagtttagca ataggaggtc ttttcatttg
     9061 tttcttcaca tttggaattt cctcccaaga agctaagaaa actgcaaagg atacaaaccc
     9121 aacttccaaa caagcaaaag atgggaaaga agcaaaggag actggaaagg aagcaaaaga
     9181 tgctgagagc tctccctctt tcaatctaga tccgccagca gaagtaaaat atgaagagca
     9241 accaaatgat ggaaaaggaa tccctgaaat ccaagcagag cttgcaaaag aagaaccata
     9301 taaaagtccg atgaaaggaa aactttctgg agactttttg agaagtatgt tactttcgcc
     9361 cgaacaccaa gacgctgtcc gcaaagtaga tcgcttgtgg ttcggagata tattccgcat
     9421 aggttttcaa gccagaccaa gacttgatta ttcatttaat cgagattttg ataagagaac
     9481 acaggatgga actaacttcg gaacacaaaa ttcacaagtc tggttcgcga ttgacccaac
     9541 acctaacgtc agcgcaaaag taactattca agatgttcgt gtttggggag gagatcaatc
     9601 tagaaaagat ggtcaacttg gatatttagg tctgtcaaat tctgcaggaa tagaagtcaa
     9661 ctcaacacct tcatctacta acgttataaa tattcaaaat agaactggaa tcagagaagg
     9721 ttttatcgtt ctaaaaaatt acgtagaagg attggagttt acgattggac ggcaggtctt
     9781 aggatttggc gataaccgct atataggagg tcgaaatgat ggacaaaccg gtaactcatt
     9841 tgatggagtg aaagcaaagt atgcttttaa taaaatgttc tctacagaag cttttacttc
     9901 tattttagca gaagaaagca atggaggtgg tggaaacaat acctcaaatg ggcagagaaa
     9961 gggaactgtg aatgatacat atctctccgg tttatacaat actctaaagt ttgaagattt
    10021 cttgattgat ttatatctat tcaatattga ccgaaagtgg gagcaatcca ttacgacaac
    10081 tccacctaca agtatggata gaactcgtca aagagatgat ttaaatacag ctggatttag
    10141 aattacaaat cgaacgaata acaatattct cccaaagttt aaatcatggg actggacagt
    10201 ggaatcgtcc atgcaattcg gaaatactgg tgttcgagtg aatgctgact gggatgtatt
    10261 tcaaatacag gcgcctaacg gtcaaagact ctactctcag agacaagaat atgattctcg
    10321 gttctttctt gctcagacag gttatacttt ctttgatcgt tttcgagtag gaattcaata
    10381 ctcagttggg acaggagata atagtcgttc tgattcaaga atgagaacct ttgatgcttc
    10441 ttttgctaca cgatctgggg ggtttccata cttcgattct ggagctggta ttaccaatac
    10501 aaccttttgg tctaatactc gtattaaatc ccttcacctc atgtggaaca cagaaaaatt
    10561 gggaagatgg attggtgtaa tctatgatac tcaaaaagat caagtgaatg atgcctggta
    10621 ttcttcaggt ggaactccca atactggact tagttatgaa aatacgacag gcggtagatt
    10681 tggtggttcc aacacactcg gtcagaaaat tggaaagcat ctcttttatg aatacaatct
    10741 aatctggcag tattatctaa aagactatgt atccatttgg gctggagcct cttatctaac
    10801 agcaggcgat tctattagag gtgttagagt aaatcctctt gctagtgatg ccacagctcg
    10861 ctactcactc gacaataagt cctatagctt tttcttattt gttcaatttg caatgtaatg
    10921 gaggtttata tgaatgattc aaaatcaaaa aaatattcaa gacagatttt atttctaatt
    10981 ttaattctcg ctctatttgg atttactaaa tgcacaaatg aattgagaaa ttcgggacta
    11041 ggccccaaaa agaaaaataa taatgtccaa ttacagtttc tgtcttatgt gtatgcagat
    11101 actctcacga atcttggagt aaattcaaga tgtgcacgct catcaggaac aattatttca
    11161 aatccagccg tagcaaatcc aaatagcact gtatataatt tgagaaattg caatataaat
    11221 aaggtagatg gtttttccac aaacagagtt acttcttcta ctaccggttt tgtaggaact
    11281 tctacttcta gcttaatcgt ttccaatgga agtcaagttg ggacagatgg aacaggcgga
    11341 aaaaggaatg cagaagttac atttacagta aatagctcta gcggctactt ggatgtaatc
    11401 gtttacggag atgggaatac caattctttt tccggaccaa catggagaat aacgtctgcc
    11461 tcaaatcagt ttaaagacca aaatggaagt tctagcaaca gtcaaattgc atacagatcc
    11521 caggatggag ttttcagagt tccaatcagt ggggctactc cgagtttgcc tgcgaaccct
    11581 attcgaacag tctctacagg atctagtaga acaatctgct tagatttttt tggaagctcc
    11641 aacactttgc aggctgcatg ggacggtagc tgcacaagcg tagcaacttc ccaaaagtct
    11701 acacgttcta gctacccaat cttaattgat ccagcagatg caaatttaac atcttcctcc
    11761 tttgctcctc aaacaacagt agggacaaag ataggattta ttttaaataa cataacactt
    11821 acaagtttca caatagcaaa tcaattagca caggaataat tttaaggaga aaataatgat
    11881 aaattttata agattaatta gcatagcaac catactcact tccggaatga ccctcttgtc
    11941 caaggataag gatataacgc ttttgaatgt atcctacgat ccaacaagag agttttatgt
    12001 tgaatacaat aaactttttg caagttattg gaattctaaa acaggggaca atgtaagaat
    12061 caaccaatca cacggaggtg gaggaaaaca agcaagagca gtgattgacg gactagaagc
    12121 ggatgtagta actctcgctg tatcgtatga tattgatgta gttgcttcta aaggtttact
    12181 agccccaact tggcaaaaga gtcttccaaa caacagcact ccctatacat ctactatcgt
    12241 atttttagta agaaagggga atcctaaatc aattaaaaat tgggatgatt taataaaacc
    12301 aaaagtaagt gtaattactc caaatccaaa aaccagtggt ggggctagat ggaactattt
    12361 agctgcgtgg ggatttgcag cgaagaaatt taacgacgat agagggaaga tagaagactt
    12421 tattaagact ttatataaaa atgttcctgt tttagattca ggtgctagag gctcaagcac
    12481 aacgtttgca caaaatggaa ttggagatgt tttactgact tgggagaatg aagcattttt
    12541 gatacaaaat gaattgggta aagataaatt cgaaatcgtg tatccatctc taagcatatt
    12601 ggctgaaccc cctgttgcgg ttgtcgaaaa aaatacagaa aagcatggca cgacagaagt
    12661 cgctagagct tatttaaaat cactctattc ggaacctgca caagagttaa ttgcaaaatt
    12721 tggttatcga cctagaagtg aggcagtctt aaaaaaatac gaatctaaat ttccaaagat
    12781 tgaactttta actatcgatg gaaattttgg tggttggaca aaagcacaga aagaacattt
    12841 tgcagatgga ggaactttcg ataaattata cgttaaatga tagagtgatg ttcagtatcg
    12901 gataaaaacg atactgaaag taattttaaa taaaaattat aaattacctc aggttaaata
    12961 aggattgcgt aaaaacttaa attggaattg gggataattc ccgttcaacc cagattttac
    13021 acaatcctat tttttaatag attcaaatca aaaatcgaat tgaatcgatt tgatcagaca
    13081 ggcttcttct taataactaa ttttctttcg atttcaaata ttttctcagg aagtttttct
    13141 tttccaagtt ttttcttatc attttctttg ataaacaaat cacttccata tttttcatac
    13201 gctgcatccg ctttgatatt cttaagccca ataaattgga gatgaacttc tccagcaggt
    13261 attccatatt tgtcgccaac atcctcagca gaaataatac ggctaatcgc tgttactgta
    13321 tccccactga tggaaggttg tgtatgatat ccttctgtat aacctaaatc ccaaagcata
    13381 ttttcagaaa tatcacgcga tgccattcca accaaccaag caaagactag tccaccataa
    13441 acaattctct ctccactcat tgcaccagtt aggcttttag aatagagttg atcataatga
    13501 agcggatgtg tatttccaac tctataggtc catgcacaat gttcatctgt aatagttcta
    13561 ccatttgcat gaatatagat ttgccctgct ttaaaagatt cgaaataagt tgattcccaa
    13621 gtagtctctc taaaatcttt tggataaagt agagaaggca attttattac cggcttgtct
    13681 gtttctggga aatgactgtt tggatttcct ggtttagagt cgccctttgg tttgccattt
    13741 gagtggttaa tcataatttt gcgctcatat tgcaaaacta aatcaccact ttggtttaag
    13801 caaagagttc gaacatgagc aataccaggt ttatctggtc ctttatcatc aacctttaat
    13861 acttttgttc tagcagataa cgtatcacca ggatagacag gtgctaaaaa ttgaacatta
    13921 taataaccta aattcgccat tgccttttca gaatcattct gcacaccgat cgacaaagcc
    13981 atattaaata ccataagcgg gcttactaac atatccttaa atccatgtgc ttgtgcatat
    14041 ggtctagaaa gaaagagagg attagcctcc ataaacgttg ttgcaaattc ttgcgcaaaa
    14101 gagaaatcta ttgtaaatgc tcttgggtga acaaatacat ctccatcttt aaattcatct
    14161 aaatatctac cgtatgtttt ttttataata gagtttgtct caacaggagt tgttggtgcc
    14221 atttctttaa atggtgcaga ttcaatttgc ttcataatct aatccttttt ttataattcg
    14281 ccttattttc taaaataaaa catcataagg cagttatgta tagatttttc atctatcctt
    14341 ttatagaaag aattttttta gtataaggcg aaccttggta gactcaccga tatgcgtttc
    14401 tttgatcaag gctcctccta tgactgccat gagagtgcta tgtagtaaca aaccttgccc
    14461 cgctcctttt tgttctttgc tttcttcata gccttttcca ttgtcttcta tccataattc
    14521 taaatttcca ttttctaata ttagatatat ttgaacttct agcgctctat ctggagatct
    14581 tgcataacga gaaatattac gaagcaattc tctcactgca taataaatca cttctagagc
    14641 tatcgattcg agtaattcta attttttttc tgcttcctct tgaatttgat attgaacaga
    14701 ttcaccacct aactctgatt taaccagatt ttcgatttcg attataaagt tagtgtgatt
    14761 taaatttgta ggatgtaagg gaattaattt taataaattg gatatttttt tatgtaagtc
    14821 agacaaagaa tttataagat tttttttagc taattcgttt gtcatagaat caatctcaat
    14881 cataatggaa tgaatctcgg gaagaatatc gtcatgtaaa actctccttg tctgatgatc
    14941 taatatttta ctatccatta attgtttctt ttgaagattt acaagcaacc tagaaatctc
    15001 tgccacagat aataaatcca ttatcttacc agatccgata cgcgctatat ctatctcttc
    15061 ttctgtataa agcgtataat cttttttgac acctaaatat aataccccta tattttcatt
    15121 tttatcttga atggtaattc ctaaaacata accagaaaac ttttccctat ttaaataaaa
    15181 caaacttttg ttttgtggaa cttgacttag aaaattgtta attttaaaat ctaatttttc
    15241 ttccgggtag aaaagaggtt ttggaatata cgatgaatag gctcctagcg gaataagaca
    15301 agcgcgagtt gtatttaagg tagtatcaca taataattgg aatgttttct caagttgaaa
    15361 ctctattcgc tgtgaggtat cagtgataga taaataaaga ttttcattaa ataaaaatgg
    15421 ctttaaagtt tttttatttt cctcttccct ctttctagaa atcatttgca cttgaacaat
    15481 cgaaaagatt gctagaaaat taattagaac ctgtttaaga tcagttgagt agcccaacaa
    15541 accactacaa accaaaataa atccatttgc aaaaacaaag atggaaatat ttttccaata
    15601 ttcctttaaa gatttcatag ccaagtatct accagtaaat acttgatagg aaataactgc
    15661 tctcccaaca attacgatag ttgccataat agcgatattt gctatcgagt ctaagaatag
    15721 taaaaatgaa ggaaagggat ttgaccgtat acttgaaatc tctacattta agtagtctct
    15781 accttgaata gacgctatac caactaaaat agataaaaag aaaagtatat aagaagaatt
    15841 caaaatagtg cgataggaag aagatttcgc aatttcttct aaactattgc tctttaattc
    15901 tgttcgcaaa agaaagagaa tggagctaag agtgcataaa ataatataac aggaataaat
    15961 ataaaaaaca gtatcaggta tttgcaagat aatatcataa acagttctat ttttttgaat
    16021 aggtataaaa taaataacga tgaaagtagt aatcaaaaaa tagattgcta atatcaaaaa
    16081 aactgtattt cgaaaactaa attttccaaa caaaaatata tcatcataaa atcgataaac
    16141 gatatatgtc caagccaatg gcaataaact aagaataata atggttggtt gaataatgga
    16201 agaagaaaga gatagaatat atataaaccc ctttcctaaa acaagtaaat gtcctataaa
    16261 aaataaagag caaagaaaaa aacttattcc tgctacaatt gcctgcctgt cctttttttc
    16321 agaataaaat aaaatcacac aacccaagta aaaggaaatt agatatatag actgagataa
    16381 aagcaaagaa atagtgttca gtattgatcc caattcactt cctctcctct ctcattcaaa
    16441 taaaatattt tccctttttc tgtccaagtg agtaatttat tctcacgaac aataagagag
    16501 gctcttgttc ttgcaacttt ttcgtctgta gcgttatccg ctaatcccca tactgtataa
    16561 atctgtccat tgattcggct tatggttctt ttgtccttta ctcctaattt aagagcaatt
    16621 tgttcattgc taagtccaac agagattagc tttgcgactt ctctttcatt tggttctaaa
    16681 agagcaaggg gtgaattctc atctgaatat tttacttctg caactcttgt ttcaatctct
    16741 ggatcaataa agctccttcc cgaataagca tctttcaata aagggagaat catagaaggc
    16801 agtaaataat ttgatttttt tacataggca taatgactta agattccaga gcgtctaaag
    16861 tcgcgaaagt aactatcgtc atcttggata gaataaaaca caacgggttt acgaggaaat
    16921 tcttttcgaa ttgtaatagc agactctatt ccatttggtc caataatttg cacatcaaat
    16981 aataatacat caacttcatg acttctacaa aactccaaag cagcagcacc attatcacaa
    17041 tcatataaga ctgaaacttt ttttgtttct tctaaaccct gcttgagtgc ctttcttaat
    17101 ttaacattat cctcaattaa aatgatttta agttgttcca acattccata caatcctttc
    17161 aataaaatat accatttata attccagtca agcacttgag tgcaatttta acaacactca
    17221 agcggtcgca tctctaaaca aaagatgtaa tattagattt attatgagag aactaattac
    17281 aataacattt acaatattag cacttgggct tttttctccg cttatgattt taatagcggt
    17341 gtgtatctat ttagaagata aaggatctat tttcttttta caggaaagga ttggtcttcg
    17401 agaaattcca tttatggtta ttaaattcag aactatgaga gatggtaaag taacatatgt
    17461 aggaaatata ctgcgaaaaa ctggactaga tgaattagct caattattca acatactaat
    17521 tggcgacatg aacatagtag gaccaagacc attgacacga tatgatattg atagacttgg
    17581 ttggaatcag tataagttta caaaaagatt tagcgttaaa ccaggattaa ccggtcttgc
    17641 tcagatctat ggaggtagag gtgcaagaat ttcaaaagta tttgaatttt cttatttgaa
    17701 actttctagt ctaagcttag atttgcggat aatctttgtt accttcttta tgaatgcttt
    17761 tggaaaaaaa aagattcgta attatctatg gaaaaagcta aaaagtagaa gaagaactcc
    17821 aagttggaaa aactggctga cctattttaa aaaaaattca ctttctccaa tgaaagaaag
    17881 tattgataca gtcaatttat cagagaggaa attagaagca cttcgctact ctctatctat
    17941 ttttcaattg ggagagtcag gagagggaag aattgcaaaa gaaatagata aatgcaatat
    18001 ctacggtgta aatgaaacat atcgagagtg tctaaagcta ttcgtaaaag aagaaggcaa
    18061 acatggacgc ctattggcgt taatggtaag aagtctagga ggaaaattag caaaacataa
    18121 ctggacagaa tctttattta gctttggaag gaggttaatg ggtattagac tcaaacttct
    18181 agttctactg gcagctgaag ctgttagtgt agtattttac aaattattta ttaaagaatt
    18241 gcctttatct ggaatgaggg ctaccttaat cgaaatctgt agagatgaag aatttcatct
    18301 ccggtttcat caggattttt tcaaaataag aatgaacaca aaattttcta aaatcatctt
    18361 taaactttta tggagaagta ttactcttac tgctttctgc gttgttgtat tagatcatag
    18421 gaaatcactt ttggtcttta atgcttctat aacaaagatt tttgaagaat ataaaagtat
    18481 agttctgaag gtagaagaag aagtttgctt taaaaataat ctttcaaagc ctttttctaa
    18541 attggtttta catctcagat agaaatctcc ttgcctgaat attctgctac aagcaaggtt
    18601 atatcgtctg taaaatgggc atccgaatta cccataaaag acaagactga gttataaata
    18661 tgattacaca tattaaaaga agtttcaaaa tgatgcttga ggctaatatc aactagcctc
    18721 tcatatccaa aaagctcatt tttattattt ctagcctctg tgactccatc cgtgtaaaga
    18781 ataattttat cccctacttc taattttgta attatctgct cagaactagg cttaaatgct
    18841 tcgcaaataa ttctaccttt tgagtttata aattgatgct cctcatttaa cccaactttt
    18901 aaaatcggta aatgtcctgc atttgcagaa gacattaaac ctttttgtaa atcaatatga
    18961 caatagacgg cagaaagaaa atgtccgctt aattttcctt gtaaagatct ttgaattctc
    19021 tgaatagact ccgcaggact taacttcatg tcataacact cctgcaaaga cattttaacc
    19081 atagttgcta aaaatgcggc aggcactcca tgacccgata catcacatat aaataagatc
    19141 aaatcatttg tatttttata atcaaaatca ataaaatcac caccgacacc catcatcggt
    19201 tgatatctaa aactcaaggt cgctcccttg atacgtggaa catcttctgg taaaagagaa
    19261 agctgaatct tctttgcaat catgatttgc ttttccaatt cttgtgattg ggattgaatc
    19321 gttctagttc tctcttctac tattttttct aagttatcat ttaaatttga aatttcgatt
    19381 attagattgt attggacttc agaagctatt tttagattat tcaccattgc attaaagctt
    19441 ttggcaagtt ttccaaactc atctttttta tcaaagtttt caatcatgat taaattgcca
    19501 gaagccaagt tattaactgc atccgacaaa acttccaaag gactaaaaaa atatttcgcc
    19561 ataaaaacag caacaagtcc agtgagacaa aaggtaaaaa tacataaaac gattgcgact
    19621 atcgccattt tagttttaaa atctagaata ggctttgaat caatttctaa tactagaatc
    19681 cctatgactt cctcttttag attgaaaatc tttgaatata cataagcttc tttcgtaaaa
    19741 atttcatctt ctgaaaatac tggttcccta gatttttctc ttaatacttt cctcaatttt
    19801 tctatttgag atttttctgc ttgatagact tgtccattca ttgttccatc atttttaatc
    19861 acataacgaa aattatcctc atctggagaa tagcttagaa tacaaatgga ttgaatgtta
    19921 ttttcactga aaataatttg gttaagaatt ttaatttttt ctttgagatc agattgattt
    19981 gattgtttct ctgactgaag aagtggatct tcttcgcctg ataaaaggga agaaccaaac
    20041 tttgctatag aggttttgtg atctttaaaa ttctggaaaa acattccgta agaaaactgg
    20101 aacataaata ccccaagcag aatcgagata attaggtcca aaagtgtaaa accgatgatt
    20161 aattttttac gaagtgttaa ttccatctag accaagttac aaatttaaaa atacttttgt
    20221 cacctatgaa aatatatttc attagataaa aaaattgcat ttaaccaact taattaacga
    20281 agacaggtag tttaacttaa gagaatcaga ggaaagcaat tattaaaacc ctgaatctat
    20341 actagaagcg aataatatct gtaaaccaat tcgatttatt tcccgcattg tcagaaactc
    20401 gcacttgaac aaggactgga tttttaatac ttcgaatcgt ttttggaatt tctagcttaa
    20461 taaaatttcg atctcgatca aaagtatatg gataagaact cccctcaagt aaaacctcaa
    20521 ttcttccact caccccagaa cccttatctg atgcatggta aaatctttct atcattgtag
    20581 aatcttgaat ctcaggtaaa ttataatgcc tataaatcaa ataaggatag ttaatcaccg
    20641 gtggagattt atctcgcata acagaaatta tccctaagcg atgaaagcta aatgaaatag
    20701 aattagtaga tttttttcca gcaataggca cccatcggtt tagaaccgta tcgtataaat
    20761 acaatgcctc ctctttatca gggaagtttc cagatagaac cccatctgct ccccctttcc
    20821 atgaaaaatt gtaagacgtt atctgatatg catctccaga gggaattagg ttggtaagat
    20881 ttacttcaga tggtatctta tctagtcttg ttattttgat tccaccatcc ccaataatgc
    20941 tagtttttga aaaattgaga gtcaactttt catcttcgga gctaaacttt gtcgctggtt
    21001 tcgtctttac ataattttca ttttttacag tcataaactt gaattttaag ctggattcat
    21061 ttccagatgc atctaatagc aatacttcag ctaacatctg ttttccctca tctactgtat
    21121 ttaaatcaat agaatgggaa tttttatcat catataaatt ataaacataa aaagctgggg
    21181 aaagagatga tttattcata tcatagagac tatctttatc tgaagcctct ttataagtca
    21241 ttctattaaa gtttctctca aataccacta gttcatttat ctttagctta actgcgtata
    21301 cattattttg atttttagaa ctaattcgat caaagcctgc cactttcaat ttaatctttc
    21361 cactcaattt tacttcttca ggatttgtaa aagagtatgt atttttagtg atcgaaatgg
    21421 ctggaaaatt tttactaaaa ccattgtcag aatcaataaa taaagactga attacaggtg
    21481 gattcttatc ttcttgttta taatttataa aactcaaagg attaaaatat tctttcccat
    21541 caaataattc taaatgcaaa tgggaaactc ctgatcccgt ttcacctgaa cgagcaattc
    21601 gatcttttga ttttacttta aagaaatcag gcttcaattt aacttgaaaa ccttcaggct
    21661 tgcctaaaag taaaagtgca ttttttaatt cttccaaacc ataaacgtca cccgatagat
    21721 cattcaaatg tccatattta gcgtaaattt tcaagcttgg ggagaaaaga tttaaactta
    21781 gcccataacc attcgcagag taactcatgg atgcaatgtt cccatcaaaa acggataaca
    21841 ctgagaaacc gttaatacca taggttttat aatcacagcc catgtgtaaa tgggagtttc
    21901 gaaattcagc aaatgttcct gatataggaa ggcgaacttg catcggccaa taaacagaat
    21961 caatcttctc tggctcaatc gcagagaggg atatctcgaa caataaatat aaagcaatca
    22021 ggaacgtttt cataatgaaa taaagattta caatagactt gctgtcaatt ttatttcctt
    22081 tatgggttta tccttcatct cttttagaaa ttttactatt ctatttattt tttcttttca
    22141 ttttttttcc tgcgcttatt tccttaagga acctggacgt cctccaaaag taaatggtgt
    22201 tttgatagat gactcttatc gtagactcta tattcataac atacaaaatg attcctatgg
    22261 aaacgcagtt cacacaatgc ttactccaat ggtaaaagca gaagtagaca gaagaggaag
    22321 atttattcaa actagagata agtcttctgc ttcatttcga ttttatgcaa gcgttataca
    22381 ctaccaaaaa gttggaaact taatggatgc atttggacag catataagtg ctgaaatctc
    22441 tttagtagtt cgtcttgaaa ttcaagaggc ggctactggg gaaaagattc cgctagagcg
    22501 agacgaaatt ttaattcgag gatattactc cgatcaaatg ggatacagag agtccgagga
    22561 acaagcacaa tctagaatgt tacagaaact agcctatcga atttcagaag aaagtgagaa
    22621 tgcatggtat tattttgtaa aagaaaaata ttataagaat gtgagtcttg aaaaaaagta
    22681 gatagaattt attcctactt taaaaaatat gtaagtcagc tatgaaaaaa atcaaacttt
    22741 ctcgtggaaa atcagctctt atcgatgacg atgacttcga aagagtgagc caatacaatt
    22801 ggtatatcaa tgaaaatgca gaaaaaacaa aactatatgc aatgcgctcc aaattaaaaa
    22861 gtgaagaagg cattgttaag ggcacgaaag tttacatgca ccgtttcatt ttaggaattg
    22921 tagagaaatc tgcagtgatc caacataaga atggaaatac attagataat cgaaaagcaa
    22981 atctagtcgt taaaaagaga actgttacta aaaaatctgc aaaataattt cattgaagaa
    23041 taacatcgat tagttctgga aattgttttg cttgatttga taaaatataa ttttcatgaa
    23101 tcgaccacag aaatacaagt ggcgcatctt ccttaattat attcagaatg tcttttggat
    23161 ttattccagc atcaggatta ttcctagttt gttttataaa ctcgtccacc gtcctatttg
    23221 cgtaaaaaga tcgattgcct ccatttccaa aagcgtcact cgctagaata ggatcaataa
    23281 agttataaac tgaatcgtag tctatatacc aagtcagtaa cgttagatct cctttctttt
    23341 ctccattttc tttgtataat tttgttttct ccattggtag aatgataata tttaaaccaa
    23401 tatctttcaa atattgagct aaaacagaac cttttgcttt attctcatca tctgctctca
    23461 ttctaagttc aattttcttt gttaaaatat ccggataaca tttagattct tctaaatact
    23521 ttctagctaa gtctttatta taagtataat agaatttgtc atttgtaaat ggttctaaat
    23581 attcatttgt aactgtcgta ttaacgtcac ttgcagaaga attaaatatt ttctctatga
    23641 tttgatttct atctacagca taattgagtg ctttacgaaa ggctagatca tagcaagaat
    23701 ttgtgtgatt tatagcaata tactgcacag actttccttt tatcgatttc ctctccttat
    23761 ttgctatcgc tggatgctct aaaagaaaat aaggaacctt aaaaatatcc aattggcttt
    23821 tttgaaataa ataaatcgca ctcgaaggtt ggtttaatac ttgtaaaaat agtctctgcg
    23881 gataaccgac agtatccgct ctatttaaag aaagctctat tgtttcattt ttattccatt
    23941 tctctaagaa gtaaattcca ctactaataa aatcaccgtt tattttgtga gattcttctc
    24001 tatagataga ggcaggtgca agcgataaaa gatctaaata ttttctatta gcctgatcaa
    24061 aagttatttc tatctgaaac ggagatagaa tttttatatc ctgtataaaa gagtaacgag
    24121 atttccttgg acctttttct gttctaagtc tatttaaaga atatttaaca tcattagcat
    24181 ttaaagtgta accttttata gtcttttcgt tctttaattg gattagaatt ttatttccat
    24241 tcaatttaaa ttcattcgct aaatctccag taatacttcc atcctttttg taatgaaata
    24301 attttacaaa taaaagcgaa ttgatttttt gagacgttag gtcaacagaa taaattggat
    24361 ctaatgtagt aggatcagat gcaataccaa cacgaactat atttgaatac tcctcttggt
    24421 tttcaaactt acagaaaagt aaaattgtga taaaaagaag ataagtaaat ttcaaaaata
    24481 aactccaaat ccaagtaaag tcattcgttc tcgcatagga ttataaatat tctcttttgt
    24541 atggaagtaa acatattgaa attcttcttc taaaaataaa taactacttt caaaattata
    24601 tctaaatcct acttttccaa aaaaccgaat ggctacacaa ttaaaaaaac aggaacctac
    24661 tcccaagcca gcactaacgt agggatcaaa tttactttcc ggagtaaaat agtattttac
    24721 cgctccatcc aaagtatcga ttgaaagatt gatcgctcta gaattttcga caaaggattg
    24781 agccatgtag atatttaaaa tcggattcga agaaccaaaa aataaactag gtaaaaaaac
    24841 gttattattc ctagatgcat ctgcttttaa agaataactc cctgttgata gtccatataa
    24901 aaatgcgata tgatcatcag gtggatgcgc ttctacataa aatcgattta tagaagattg
    24961 gctagaagat tgaatcgccg ctgaatttaa ttgatcattt aaaacataaa gattaaatag
    25021 agaactagaa tttagattac ctgaaagaga agaagcaaag cccggtgctg cctgtgcaga
    25081 actaaataaa gctaaaacaa caaattgcga cagagatgtt gctggatcat atcgatcttt
    25141 taatacacct aattgtccaa ccccatttcc aatgataaat tgagcagaaa gtcttttctc
    25201 atcttgattc ttctttgtat tcgtttcagt ttctacaatc gacttttcag attcagagtc
    25261 ctgagggaat aacgacaaaa acgaaattaa aaaaaagagg attagtttca acatgatgaa
    25321 actatgagtg agctagttat aaaatcaatc aatcataaaa ttagcagcca atataaacct
    25381 gaaaaagttc aatacgagaa tgaatagtgc taccatttct cacttttcga atttctcttt
    25441 cgtagtcttc tctaactttc atgatttcat ttttagtgcg gttaataaca gatttacgct
    25501 ctggacgagg atcccacttt tcttgggctt cttgcctatt taatttttct tgtaattgtc
    25561 taattacttt ttggcttgtt acttcaattt taaattcttc tttcttaaaa atagaaagag
    25621 tattttttac taattcatcc tttcttgttt caacaatatt taataatttt tcatagcaac
    25681 gaataaagcc tgattccact cggagcgggt tttccgacaa attgatattt gccgccaaaa
    25741 cattatcttg aataaattca ggaatcgtat ttagctctga aaccatatca atcgaagaat
    25801 catattctaa atagaaaatc tcagttcttt taaggctaaa ttgaaactca actagaaata
    25861 caaagaatat tttagattca tattcaggat aagaaaaata ggttctagca aaaccgctct
    25921 ttgtatcaat taaatactga atggactttt caacaagagg atgtccaaat gctaaaaact
    25981 ctagagaatc atttactaac gcatcttccg aaaagaaagt tccatgctta acttccttac
    26041 cattttgaat caaatactgc ctattattct ctggattgat ttgtaagttt gagccaaatt
    26101 catttggaaa atattttgca aaggatatca aaaattcttc taggtgagta ttattgtatt
    26161 gtcgctcact taatgtatga ttgtaataat cttgtagatt aaaatcgatc agcttaggag
    26221 ttactagatc atttagttta ttaaatcctt gttctgcaac ttttatacgc aagtccattt
    26281 caatttcgat ttcttctttt gtctttgagc ctgtaataaa acgcataaaa ctagaattga
    26341 aatctagctc gtcctctatt gttccaagta aagcgtctga cgcacctatc gaatcttcaa
    26401 aaagttttat tttattcgtc aaaacttcta agattctttc tgcaactgta tctttactcg
    26461 caaaattaaa aatatataca tctttcttct gaccaaatct atgaatacgc ccaattcgct
    26521 gttcaatttt aagtggactc caaggtaagt cataattgaa gagaacattg gagaattgta
    26581 aatttctacc ttctcctcct gcttccgtgc atattaatat ttcaaaatct tctttgaact
    26641 tctgaatcgc ctcttctttt ccatctgccg aaagagatcc atgaaataga actactttaa
    26701 aatcatctag aatgctataa agataatcct gagtgcttct aaattgagta aaaataataa
    26761 actttttatg accttcttta cgcaatcgat agagagtctc ttttaacttc atagattttc
    26821 tatcttcttt gatcgacttt cctaaaagta tgagtcgatt taaagatagt aattctcgtc
    26881 taacgttctg aagctctata tgagcagagt catctaaacc agaaataaaa tcttccacac
    26941 cttctgtctc gtctaaatcc caatcttcta actcttccgc tttcaattta aatttatgca
    27001 ttcttgactc gatcataaat ttacgacgag tcaatgcaga aagtaaagcg acatgggatg
    27061 aatcaagcaa tttctgaaac acaatcatta caaaaccgat tgctcgattt tttgtgttca
    27121 tcgcaaggtt gtattccctt cttacatatt cagttgtctc ttcataaaat tgtctttctg
    27181 catcggataa atcgatcttt actgttttag caaaacgttt tgtaaatccg ccaatttcaa
    27241 cttttcttct acgaagtaag actttagaga ttttctcttt gagatcggct ttactcccaa
    27301 caacatagtc attgataaat gtatgatatg gtcctaaaat atttggatct actaaatgca
    27361 tcaaataaaa gagttcttcc aattttccac gaaatggagt agcagttagt aaaagcaaac
    27421 attcacactt cttagaaact ttttccgcaa atacataagc gcgagtaatt ttagaatagt
    27481 ctctcctcaa tcgatgagct tcatcaaata taactatatc ccatttcttt ttaagaatat
    27541 cttccgcata acgtggattc tttataaaat caactgaagt aatgacatga ttgaagtttt
    27601 gccaattgtt ttcatcatca gatataaaat tttttcgttt aatgatagta aagtcttcat
    27661 tgaatttatt ttttaattct tgtttccact gaactaataa aggagatgga gtaacaatca
    27721 aaacacgttt atacgcattt cgaaaaatta attctttcat gataagtgcg gcttctattg
    27781 tttttcctaa accaacttca tctgctaaaa taaaacgtgg tttgagacta ttcacgacaa
    27841 tataagtaga ttcaatttgg tgtggaagaa gtctagttct agagttagac aatgaggaga
    27901 gtttatcaaa agcatgagtt aattttaact ccaatgcctt taaagaaaga ttcatttcaa
    27961 attttcgctc ttctttagtg tataacgctt ttaaattaga agaaacatat tgtagatttg
    28021 gatcggaatg atcaattaat aattcttttc caacggagag gaaatacact gttagccccg
    28081 ttttagttgc ggatataatt tttccaaaac caaattcagg atgagattta aggtaaacta
    28141 cctcaccttt tacttccttc gttggtattt tttcgaaatc aagtgtaaac tgaaccagcg
    28201 actccatcaa actccccgaa cttcccccca aagatactta tgcaattgta tagaaaatct
    28261 ggctccttct atgtttgcat ccttcagcca atctgcgagt aattgtggct tcaattctgt
    28321 gtaaacagaa gaaaatatta gattttggtt tagttgaaac tcttgaatca cttctaggct
    28381 tcgatcaaaa tccttacgat cacgaacaac aaacttgact tcatccaaag agttatttct
    28441 ttctcgaata aaatgaaagt tgtcagggtt catctggtct tccataccag aacccggcaa
    28501 tttataatcg attgaaaata ccatattttg caaattttga atgtcttcct tcccgtttgt
    28561 ttccacacgc gtaaaagcaa atttagaatt atgtctcttt tcataaacta tttttgcaaa
    28621 attagaacaa aaaaattttc gatctccttc cagaggctct ccacccgtta aaagaatctg
    28681 agtcggagtt gaagaaaaag attctacttt ttccaatatc tcagtcaggc tcatagaagt
    28741 ccctgcattg tgagaaagtg cataacctgt gtcacaccaa agcttacgag atccatccat
    28801 tccacaacgc aacccacacc ctgcaaagcg aacaaagacg gtcggaactc cttgtgaaac
    28861 tccctcaccc gacaaagaat agaatatctc atttacgatt gcgttcacat gatttatgtc
    28921 gtaaaaaaaa agagtttgga aaaggaaatt tattccagat cagttatatc accaatatct
    28981 gaacaataaa aaagaaagac ggattcgaat agcatcgggt ggaattcgag ggtgataaag
    29041 ggaaacctca tcttatatca aactcacgtt aactaaatat attataagga aacatcttta
    29101 gaatcaaaat tagtttccca caatgacttt aaatgagtag cctttggttt actaaataga
    29161 tggaagtgtg gcgcactgag aggtgcttag agtaaacctt attttaaaat ctttttaggt
    29221 aagacaagag ccgtctaccc atcttcttta tcgatgagaa ttagttttaa atgcgtttag
    29281 gaaagatatg gatcgtatac gaaagatcac aaggaggcaa attgatgtta ctctagtcaa
    29341 tatgaaccca agcatttttt tagaatcatt gcaattcgaa gttcataaaa aaagacattc
    29401 aatcataact gagtttacta tcccctgtaa ttggaatttt ttctccaaga aatcttggct
    29461 ttaaatttaa actgacttct agatatgctt tcacgcaggc aaaaatatct cggaaactat
    29521 ttttcaaatt ttgaaatgtg ttgtattggt agttttcaga aatagcaccg taagccgaaa
    29581 gaccttttga cctagcaata taaatagccc taggcaaatg aaactcttga gagactaaga
    29641 tcacatcctg aacagcaaaa atttcttttg ctctgacaat cgaatcatat gtatcaaatc
    29701 cagcatgatc caaaaaaata tcttcctcag gaatgcctaa atttttgaga tacgctttca
    29761 tattgtttac ttcatcataa ttttttcgac catgatcgcc actgagtaaa aaacgtttga
    29821 tctttttatt tcgatagagt gtgacagctg ttaagaggcg atcttctaaa acgtcagatg
    29881 gtataccagt tttctttacg aaagcaccta agactagccc cgtataacag tttggaatct
    29941 tattcaaatc cctaaataaa aaagattcag catttttatg aattgaataa cttaaataga
    30001 gtgcaaataa aaaagagaga attaaaaagc taatacaaat aactgctatt ttaaaataaa
    30061 aatttttcat cattcttccc attccaaaat ttttgaatta gaataaaaca aaaaaaattt
    30121 ccctttcgct ctattgatac cacagtctat tgatttaatt tattagaaat ctctaatatt
    30181 cacaaaagta tcctttgtat taaattttct aatcacaatt aataactaat ctcgtttgaa
    30241 aggattgagt tctttctttt tatatgaatc ctaaatccta gccatttcta ttgacaaaaa
    30301 cttatttttt actgaaatga cgatagtaat ctaagggcat tctatgaatc aaaaaacaac
    30361 tggctttgaa aaatgtgaag ttgatctaag taatacgggg tttatccaag gttttggatg
    30421 tttattaaga gtcagttaca aaacgagatc gattgtaagt gtttctaaga attcaagcga
    30481 attcctacaa aaagaatcct taatcggaaa aacactgaca gatgtttttg gagaagatat
    30541 ggaaagtcga ctctggaatt atccatttaa agcaccgatc aatgaatctt gggagaattt
    30601 taaaatctac ggacaatccg atgagtttgg tattatacta gaaattgagg ctaataccga
    30661 tttgccaaaa cgcaagcccc gatttgtttt atttggatcc aaaacgagag aagaaattat
    30721 caataaaact ttaactcgtt taaaagaaga aagcggtttt gagagagtta tgctctatga
    30781 atttttaccc gatggaagcg gtcaagtaac agccgagctt tatagtggaa gtggggataa
    30841 ttatctagga ttaagatatc ctgcaacaga tataccggct gtcgcacgaa aactatatct
    30901 acgcaatgct tataggtata tatatgatgc aacacaagaa tcaattccaa ttctcaatgc
    30961 aaataatgag atagatacaa ccgtcaactt aagttattgt tttttaagaa atgtttctcc
    31021 atttcataaa gaatatctaa aaaacatggg agtcaaatgt gctctttctt tctcccttgt
    31081 cagggaagat cgtttaattg gaatgttctc tttgcataat attgaaccta aatttgtttc
    31141 ccatgaatct agattacaaa tggttcaaat cacaaaagaa ttcttacata atttaagcat
    31201 tcgagaaagc gaagaaagaa tgcagttttt agatggctac cgtagagaag taacaaactt
    31261 tatccaaagt gtaaatcatg gtcatgaaag agaaaatgaa atcttaggat tcctacaatt
    31321 agttggcgca actggactcg caataaaaat gggagaaaat tggacagctt acggaaaagt
    31381 tcaaatcacc gaagaactta aatcaatcga agaaagtata acccaagtag aggcaaaagg
    31441 tatttttacg acagactgta tatcttctat ttatattgga atggaatcag acaaagactt
    31501 tgcatccggt gttctatgtg tatggtatac tgatttatca actaaagaaa aaaaatcttt
    31561 tctctttgta aaaccagaag ttttacaaga agtagtttgg ggaagtcgtg tagagttata
    31621 tcctggagat gtaaatcctc tcaactcttt tggacaatgg aaagaattaa tgagcatgca
    31681 tagtaatcct tggtctaggc aatcgcataa cgctgcacaa gcaattcttt atgtaggatt
    31741 atcaaagtcc tttcctattt agcattcatt ttttataaaa taataaaaaa gtagaataac
    31801 cctaagtgct actttttgat atatagtatt atatacggag gctaactaat aataacctct
    31861 ataattctac tcattgatca tttattttcg aacataagtc aaaactgatt attttttttt
    31921 aatctttaca aaactagttt acgctttcga tggaaacaat tacaagagag ataaggagta
    31981 attaattgaa aagaaagaga attctattag ctgaagacga tcaatatact ataaaatacc
    32041 tttctgattt aattagtaat catggatatc atattgatgc agcaagagat ggtctcgaag
    32101 cacttaatta ttacaaacaa gacaattacc cagtaatcct tacggattta aatatgccta
    32161 aactcaatgg gctagatctt gtcaaagaaa ttcgatccct aaatgaaatc tcagttatta
    32221 tagttttatc aaacgaaacg gacattgaaa gggtaatctc tgttatgcgc gagggtgttt
    32281 acgattattt aataaaacct ccaagaccgg aagatatttt aattaaaatt aatcacgcct
    32341 acaagctcag tcaactcaat cttatgaaat ttaatataga aaaagaaaga gaattgagat
    32401 tgaacaatca actcaattgg atgaattggg tcaaaacaaa acaatcttcc cagaaagaaa
    32461 cctacgaaac agtgcttcaa aacttaaaac acagtctaaa ccaaggagct gggataggaa
    32521 ctattgtcag tttaatagat atactcaaat caaatgggaa actggaaaat gataaatata
    32581 tcttccccaa aaagataatt gaactactct atgaaaacgg aaagtttgca tcgaatacac
    32641 tagaaagggt tgaactcatt gcaggcctag gtgaaagaga aattcattta gaacccgtct
    32701 ctgtaaatga tttaatagac tttttaaata aaattatctt agctcaaact gaactactca
    32761 caataaaaca gcagagaatc gttttatcaa atacttcaaa tacaaaatca gaaagattcg
    32821 ttcgcttaga tcagaactta tttcaattat taatctcaga aattctagtc aacgcaatga
    32881 aattttcaga agaaaatact gctatttatg tattattttt catcggggta aatctaacga
    32941 taaccgtttt atctcctccg aattcaaatg caaatacaat tggtgttccc cccgaaatgg
    33001 agtctctatg cctagaacca ttttttagag tatccaatgt aatcgatgag cgatacagga
    33061 cattagaaat gggtttagga cttacaatgg cgcacaaaat aatgaaaatt cattcgggaa
    33121 atcttgaaat tacaaatatt aaaaactatt tagaagacag agatgtattt ccagtaaaag
    33181 tgcaaacaac tctaacattt ccactcgaca cacaattaaa attttcatga aacca
//
LOCUS       DAOQOS010000055        33155 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_582_length_33155_cov_6.765166, whole genome shotgun sequence.
ACCESSION   DAOQOS010000055 DAOQOS010000000
VERSION     DAOQOS010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 33155)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33155)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33155
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_582_length_33155_cov_6.765166"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1056
                     /locus_tag="PLF66_13295"
     CDS             <1..1056
                     /locus_tag="PLF66_13295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HMZ65068.1"
                     /translation="EFTSDYQSKKTLPQVLIYVSLDSFEFDLLTDIYRTDLMNSGDPY
                     EVVVYVAETGDLEKLFSEMFNLSMFSPQKLLIIKSGSDFFKGILTAGKKELYESFRRN
                     MPNLGDRVYVLVHYDAKDIPAKLGNLFDNRFGLIKNRSFYAEERRPALEAILKTEKVQ
                     LEEHAKDELIHRIPPNTGAYIKAIRKLKLMLNKKEFSLADVEEVLFNRSEFNPFQLVD
                     LLFSNNKAEFFRELSKLKPSAESNFVFLSLLTALLNRTDEVRKAKVLFKRFKDENDSE
                     FFKYLGMASYSDGRKKFIKSRLRRETRLFSTRALDTLYTLILSFNKKAKTSSVKDDNN
                     YSVIREFDKLFDLLASS"
     gene            1165..1416
                     /locus_tag="PLF66_13300"
     CDS             1165..1416
                     /locus_tag="PLF66_13300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65069.1"
                     /translation="MIQELKPLSNAHFIPSSKSSYSNSDLFHMHKTLVQCAESFNEYL
                     TKSKEPVRFTDNVVPLKKEFLKFIQTQEKSTLFDDFLNF"
     gene            1772..2296
                     /locus_tag="PLF66_13305"
     CDS             1772..2296
                     /locus_tag="PLF66_13305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020777655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPP family protein"
                     /protein_id="HMZ65070.1"
                     /translation="MKKRFYDLPRKLKAPLRSHSAPLSWKYAIWSFISGTVSILAILW
                     VTQITGYPLLIGSFGATAVLLFGVPDSALTQPRNLIGGHFISAVIAILLVQFFGSGFW
                     VTGLSVGLSIFAMYLTRTLHPPGGATALIGVMGNCTPMFLFVPVLAGVLILFFIALFT
                     NNFVYHRQYPKHWL"
     gene            complement(2431..2661)
                     /locus_tag="PLF66_13310"
     CDS             complement(2431..2661)
                     /locus_tag="PLF66_13310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65071.1"
                     /translation="MELTAFESRISLELSLNEKDSRRMDRVIADLSENIGMSKQEILD
                     FLIYGSTKELEALHISYDWEAFRRKIQSRLKK"
     gene            complement(2674..3018)
                     /locus_tag="PLF66_13315"
     CDS             complement(2674..3018)
                     /locus_tag="PLF66_13315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HMZ65072.1"
                     /translation="MSQQVRKSKRFYIKDSDNFIVQLETNPGKTVNCFLNDISANGAC
                     IILDKTVFVQREKQYPFVFLKKKEDGSLEKFATATGKMAWYLTKEFRNEDMMYLGIEF
                     SNEINLPPYITN"
     gene            3384..4232
                     /locus_tag="PLF66_13320"
     CDS             3384..4232
                     /locus_tag="PLF66_13320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004448743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HMZ65073.1"
                     /translation="MSNWNFLKQKIENDDFIIDCRNQAAYEESTIKGAYYFPFIKKAF
                     GSDAESQKKMYNPLKTILNLVQEANKSRVIVFDEGMGMFSARMVYLLRAIGFREAYML
                     ALKWPFEGDTEPGKHIIEAEPTEKAKPLNGVVDKAFMEKNLTRLQIFDTRTKEEYDGK
                     LPRLTSPEPGTLSGRLPGAFLWDWQTFYDQEGFVISRDIFIKRLQGFPFMPERTTVLY
                     DYNGARSCLCALMLKEVGYQDVHTYQGSWFEWRKSNLPKQAVSLYGASAASAAAPRVG
                     GIDKKK"
     gene            4250..6562
                     /locus_tag="PLF66_13325"
     CDS             4250..6562
                     /locus_tag="PLF66_13325"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF007746.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HMZ65074.1"
                     /translation="MIKKILLGIIISFYFQCSMGQPSQVRVLKEVNTKGEIGSDIQYA
                     IEDNSLRVFNRKFSFVEFPTDRSFLTIAKGSPTVKYHLAAFKFKDNFTATCRNQSIDD
                     IEKIENGIKISGILHDKDCYVKYDIVLKAENSHTLNFDVNISDKSYNRINLVYRSTKD
                     EQFFGFGEQYTHFNMKGKNPFIFTEEQGIGRGDQPITFAANLTEGAGGNEYTSYAPMP
                     HYITTNHRSVYFENSAYSKFNLQNPNFVKVLFWENNLKGTFWVSDKPLELIEYYTAKT
                     GRYPALPDWAYGTWIGLQGGTGKVKKVVDHALAAGNPITAVWIQDWVGRRVTGFGDQL
                     KWRWYAQEESELKDSNGNPEPSYPDFKNFTAEMNRKGVKVLGYINSFLADANPKNSRK
                     QPIKEPNSCGYKLFIPFYGCNPEREPDSFTNPMLEEAKAKGYLVKNQAGEDYIIESVG
                     FPAYLIDLTNPEAVKWTKNIIKKNMIDQGLSGWMADFGEWLPFDAKLYSGISAELYHN
                     IYPVDWARINREAIQEAGKEGEIVFFTRAGYTGSNRYSTAFWLGDQMVSFGTNDGLAS
                     TVVGLNSGGISGIAINHSDIGGYTGIKNPPWYFPNYDRTEELNRRWIELNAFTPIYRT
                     HEGNVPSQFNQVYSDVQMTRIFARFGKIHYALKDYFTFLVAEAKEKGYPVIRHPYLNY
                     PSDPNTYDLKHQFMVGEDLLVLPVVEKGETTVTGYFPKGKWKHAFTGKTIIGGRTFTV
                     PAPLGEPAVYVKEGGAWSDRVFTSIQGALK"
     gene            complement(6895..9558)
                     /locus_tag="PLF66_13330"
     CDS             complement(6895..9558)
                     /locus_tag="PLF66_13330"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HMZ65075.1"
                     /translation="MKNLSWRIILPGIRAKLSLFTILFVGIIIFVMSQLFIRQQKMTL
                     EESYEKEITASKSYIENVVSDLEGISRSLILIEGFRDQIKENQKALAALKKTYFVEEK
                     SFMGLFKINRDFGLKKLGLIQSKKSARKIDTFFSIYYTEKDIKELEANIKTQFKDKDG
                     KPISDKVFSDLQRYANQVVKAESLLEKNLENKAADSVIAANRKNLSTQKAQLRKQILN
                     QFQEEQKRKIKELGLPTTKIRIQSFSLLSQGDQVEAEIGFDTNIFETKGEVNSKSIDD
                     YLQNGFNQQVGILAKQDLSATSKDKFILDQKNYQTQSDLFYKNHLTVETAEKLNSKYL
                     TSEWKEYFEKEKEIQTKFKEISDKLKARIEVLKADKTRVSPSRDLEFRKLYIEYAQNL
                     QKREALVNEITKKIPNLENLEKTNLDFSNEQSLHIKNAILNLRDVALYEDIILKYKSN
                     PTAYLEYASNEKLRQEAKIKWKKMRQWIMDANSETVPADLKKMITEGTISKSRSEAEE
                     LMWELDANPIYSEDEKNLAKELQAANQIGFTRTVIDMQEGYNRIEEQKNKILYTSYFI
                     GVVAFFLALYLSDRMVRKIKRIIHSAAELGQGNLDTKFQHGGSDEFGVLTTALNQMTV
                     DLKHRQEMLIEYAAAEEIQKGLLPVEMPSNANGILEFGNFYKSMSGVGGDYFDFMNSG
                     EDKIVFCIGDVSNHGIGPALVMVALRSQLQSLVRNGETDLRKILLSMNEQIYADTPSH
                     IFVTFFLGIYDKKTGKVNYMNCGHSKPFLYRAISNKVESLPSGGMPLGAIDNDIFDTT
                     IEEESIQLEKGDLFFQYTDGVNEAMNGKSELFGSDRMEEIILKYGKESPEKVTNEIAK
                     QVEIFSGKKIFCTGPSELNDDIAMIAFRRVN"
     gene            complement(9545..9976)
                     /locus_tag="PLF66_13335"
     CDS             complement(9545..9976)
                     /locus_tag="PLF66_13335"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HMZ65076.1"
                     /translation="MREINFQFPNQLSILSNVRTKINDFIGSDLDNILKNRIILSIDE
                     AISNIIEHGFPEVKDSLISVYARLEKDKITFVLEDEGIPFNPLTKPPVDVDEHLELGE
                     DGGMGIHIVQKIMNVEYERVHLKNRLTLTKNFTEETEFEKS"
     gene            complement(9973..10305)
                     /locus_tag="PLF66_13340"
     CDS             complement(9973..10305)
                     /locus_tag="PLF66_13340"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="This anti-anti-sigma factor, or anti-sigma factor
                     antagonist, belongs to a family that includes
                     characterized members SpoIIAA, RsbV, RsfA, and RsfB;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0045152 - antisigma factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor antagonist"
                     /protein_id="HMZ65077.1"
                     /translation="MEITESESKNIWILTLNGAMDAHTSPAIKTKLETGIAAGKTKIV
                     VNMKDVSYLSSAGAGALLAGLTASRKKSGELRLCSFQAGVKDAFDVMGFSKAFKVFPD
                     LEKALDGF"
     gene            complement(10324..11238)
                     /locus_tag="PLF66_13345"
     CDS             complement(10324..11238)
                     /locus_tag="PLF66_13345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65078.1"
                     /translation="MDTQKISKVTPTLAEELSRYIRLLAKAGKGSFHRHLYQPLVYAG
                     WERQMKYNDSKFIMARIDEARSDLAKLNTIGPLCKRLISQSLLESASSLGNSSIFFLD
                     RMGSIPVLAKSEEGVEFVTIIKDPLTEFNLDNQTKKEKSFEDKLENISNQELTDALNP
                     VELGKKEVIVKIQKEALALFNQISIYSNKGDIHRCRKLIANYLIQYAEQEDNNRDEVE
                     KVIEALESRSPGFTSELNDFIAINLYYSIFSGISEGDLKKAIQAIRKYAFIFQGNPDI
                     KYFMEIDELERKLQAVITKKDLWKQLRK"
     gene            complement(11456..12004)
                     /locus_tag="PLF66_13350"
     CDS             complement(11456..12004)
                     /locus_tag="PLF66_13350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008589164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HMZ65079.1"
                     /translation="MRKIIAALNMTLDGFCDHTAGIPDEEIHQHYTDLLGQGDAILYG
                     RITYELMEFWKPFIENPSDEKFMNEFATAIDRIPKIIFSHSLKSIDWKSARLANRDLK
                     ETVLELKQQSGKDILVGSRSLFIQLLNLGLLDELQICIHPVVIGKGLPLFDHINDRTL
                     LKLTKTKTFGSGAILIYYEPSR"
     gene            12735..13067
                     /locus_tag="PLF66_13355"
     CDS             12735..13067
                     /locus_tag="PLF66_13355"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HMZ65080.1"
                     /translation="MQNNVTLKQNLKVNDNFIYSFCQKWKIDEFSFFGSVLRNDFSTE
                     SDIDVMVAFSQDSRWSLFDLMDMREELENQFNRKVDLINKKAVERSKNPYRKNNILNS
                     YEVVYAKK"
     gene            13054..13389
                     /locus_tag="PLF66_13360"
     CDS             13054..13389
                     /locus_tag="PLF66_13360"
                     /inference="COORDINATES: protein motif:HMM:NF014043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HMZ65081.1"
                     /translation="MQKNDLSHVMDIFNACNNIIAFSKGISHEEFEEDIKTQSAILHQ
                     FMIMGEATKRLTEEFKSEHNSIPWRKIAGFRDILIHQYDNIIPDEVWRIIETSVPDLA
                     RDLATLLIS"
     gene            complement(13498..13737)
                     /locus_tag="PLF66_13365"
     CDS             complement(13498..13737)
                     /locus_tag="PLF66_13365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65082.1"
                     /translation="MKTIQSQIPDFLFTQIENIARKENISIDQIVSMAISALLPIITM
                     QENIDSRAKRGSWSHFQSILEKVPAVEPDALDKLD"
     gene            complement(13734..14162)
                     /locus_tag="PLF66_13370"
     CDS             complement(13734..14162)
                     /locus_tag="PLF66_13370"
                     /inference="COORDINATES: protein motif:HMM:NF024861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HMZ65083.1"
                     /translation="MKKYKIIIDTNVLVSGLKSRLGASYKLLSSLNDERIQLFLSNSL
                     LFEYEEILKRNNHLVNLDLNEIDSLLDDICAIASTKNIFYLWRPTTSDPDDDFIVDLA
                     IASNIDYIITFNVSNFLNAKKMNIKTITPKEFLQLLGGIQ"
     gene            complement(14159..14296)
                     /locus_tag="PLF66_13375"
     CDS             complement(14159..14296)
                     /locus_tag="PLF66_13375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65084.1"
                     /translation="MKTLLAKEKDLFPDDIDIEYFDTGEFKVLTEDEIKSIISMEGIR
                     T"
     gene            complement(14696..15622)
                     /locus_tag="PLF66_13380"
     CDS             complement(14696..15622)
                     /locus_tag="PLF66_13380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016549642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ kinase"
                     /protein_id="HMZ65085.1"
                     /translation="MDIEKIVLVIRATRLQESVQRFSTKAQAKFFVKSRGQSFDDYEK
                     EDSNYQYARDQVTHAIPSDIKLQVIDRSFLPNFVFGPKDIVMTLGQDGLVVNTAKYLN
                     GQPIVAVNPDPERFDGILLPFQISTLPKAMHSLLKGSPRIRNITMGKVQLNDGQSLFA
                     FNDFFVGARSHISARYTIKFRGREERHSSSGVIVSTPAGSTGWLSSVFNMAKGIANFR
                     GDNSDLSPSRLEWDERRMIFVVREPFKSKWSGSDLVAGEISEQNNIILESHMPESGVI
                     FSDGMESDFLEFNSGTTATIALAEKFTRLVVG"
     gene            15989..19189
                     /locus_tag="PLF66_13385"
     CDS             15989..19189
                     /locus_tag="PLF66_13385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016550697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HMZ65086.1"
                     /translation="MEFLEPYTKYILFNFFSFGTLLVTIFTFAFAYFFLTLPNKSEST
                     LHLGIGFLFLAFFNTGYFFAASYYHPFAAYHRWFTGVFILPAILHFGQFFFKYPRDTN
                     PKLSKLVFRIMWAVAILGAILFISGTWNADRKFHFTGHYWDFDAEPISRMLAVLIAIY
                     SIISFILIGAWKVYVVKTRERWVILKILIAMLIAAVTPNVTNIMSRDGAIERSAYLIA
                     LTLFLVIGFFIISIVYINSTKDRTTFMVKIVGLTLVTLLLILQAANFYSIRDKEVDYD
                     TLKSENMSRIIEGGDKSKDTKYFIQLNLDELEIKKKDYPSSINLDLPLVEVDFRNTVI
                     YEDIKNLPESNFRSALNEYLFNTHPYFEGFKNSIGEYLVRNKDLGDSDLKDAIFPFLD
                     SLNKFSFIHSNKISQLDEKDFCQAITKYLNANALKNFKIAIEKNINQCKINGRLLDSA
                     SHRKEINKYFRHFQPANTRRFRKSIDEYGNQKHYIAYTHYEVYTQVVSEVGYSYLGYR
                     QYMHTSASNQQFILILVVCIVIGVYPLFFRGSLVTPLWDLVHALEKVNQGYLDVEVPI
                     KVNDEIGFLSDSFNAMVISIKYARSELQHYADKLEDKVQERTKEVQEKMDEVQKLKVQ
                     QDGDYFLTSLLAKPLFMNANKSEITKTEFFIKQKKNFEFKGKQVEIGGDICVTGNLRF
                     GTKLKHTRYTMAMNADAMGKSMQGAGGSIVMGVVMNSLMARSAAKDWVVDKAPEVWLT
                     DVYYEIHRVFKTFNGSMVISCTVMLINDETGETYYFNAEHPYSVIYRNGRAEFIEDRL
                     NLRKLGLDSEIPFQVFKIQLEPGDIILLASDGRDDIDLTPEEPVRTINEDDTLFLKHV
                     VAGRADIQEIYHSLQRSGEFIDDFSILRIAYREAVVHYANPLDEPGIILPKDIDHDDL
                     ESVYSECKKLLRDNKDEAARQLLQITYNRSEANQNNLKINKLYGLLSFKARDYVSAVD
                     VIRRYLKDDPAYEEFWYYLSMAEKKLGHYNMAMEAGEKLRERNPDHTLNLLNLADVNR
                     LMGKRDASIALTELVLKLEPDNTGANRLMQILTSIL"
     gene            19186..20121
                     /locus_tag="PLF66_13390"
     CDS             19186..20121
                     /locus_tag="PLF66_13390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65087.1"
                     /translation="MIPEFPEDIVYLEKSDRHPNLYDVSLSNIDKLRDYDSIEVHIVL
                     YPYSRRITSEHISFFPFEEYVKDVQSNQLSAYSKIQLKFHKFFGVALGSVIAGFFYKY
                     KPEDLFSVESVVAVFAAYAIGKELWADLERFFINISKRWRLRYTEDYYKYELEKFTTM
                     AHYSSLAKRKRYGKVTLLPEKINFLELSNSQTLRMHFNMKDIKPAEAKSAHIFSIHIE
                     PSLQSDFEKYGHLFGVKLSLNKKTLGFTKRLELFQSLHNREKGCLDDEEVWSANSIFC
                     RKAITLGKIKFFVGRDLLSGKSLVRYVEQKFRKKR"
     gene            complement(20269..21591)
                     /locus_tag="PLF66_13395"
     CDS             complement(20269..21591)
                     /locus_tag="PLF66_13395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65088.1"
                     /translation="MAATAELTENTITHWTDKNPRKFSHADRSTVTLFTTGFPAHTNA
                     LLVDAVALAGINLEILEEPNSEALQLGREFGNRGQCNPTYFTVGNLIKKLIHMRDKEG
                     ISTEDIINNYGFITAGGCGPCRMGMYATEYRKALRDSGFEGFRVLLLTRSGDVDDVLM
                     EGLKMGNVDLLRGFGAIMIGDLLNAVNVRKRPFEIEKGSVDRTMLEVRQTIKTHIRKK
                     QMWRGLWKARKQIKGMKLDTSKKKPLVHIMGEFWAKTTDGEGNYNLYRFIEQEGGVVS
                     SESLIDWLMHQLWRARWMEKRKRESFWMSSSEMKILLVEKLVKTIFKMMGLVLGETVK
                     ISDHDMLANISHEHFDLHVDGGEDHMEVAHHIHISKHKLADLIISVKPFTCLSSSAVS
                     DGVQSRISDLYPQVEFIAVETNGDGAANFYSRIQMAMHKVINQMKAGN"
     gene            complement(21498..24806)
                     /locus_tag="PLF66_13400"
     CDS             complement(21498..24806)
                     /locus_tag="PLF66_13400"
                     /inference="COORDINATES: protein motif:HMM:NF013986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HMZ65089.1"
                     /translation="MINKNSEYLLGIDVGSTTVKIVLTDKNLNILFTRYERHHTKQGQ
                     CVLAFLKTLEENFPSFKPGNVSIFMTGSGAVTLAPKIGARFIQEVNAVAMAVEKSHPD
                     AESVIELGGQDAKVIIFKENPGTGKRILTFMNDKCASGTGATIDKCMIKVGMDPSELP
                     KIEFNPEKLYHVAAKCGVFAETDIVNLIKSGTAQHDIMNSLADAIVNQNLSVLTRGHT
                     LKDKIILLGGPNHYLPFLVQCWKYRIKQIWKERGFQDLYEGNIEEQVIVPENSLYYAA
                     LGSCYYGASSHSEEYKGYKELEEFISGGRKAELKNIAGDPLIQDSEELTQFKEEYAIP
                     NFTTPNYQPGQTVRAFLGVDGGSTSTKCALLDEDGKLIIKEYTLSKGNPFEDFKVLID
                     KIREKTSKKGVQLDVIGFGVTGYAANIFEKVFSADVNVIETVAHMKSSQNYFGDIDVI
                     CDIGGQDIKVLFMKSQELSNFKLSNQCSAGNGMILQGMAEQFGHSVREYADIAFKAEL
                     APVFSYGCGVFLDTDRVNFQKQGFAKEEIMAGLAQVLPKNIWQYVVQVPRLEQFGNKF
                     VLQGGTQYNLAALKAQVDYIKKRVKNAKVFVHPHPGEAGAIGAALEARETVMMRGYTK
                     FAGLDEAYDIQYSSKSDESTRCRFCSNSCSRTFIDTLTPSGQKVRYISGFSCDKGTVE
                     SKEELKELMAKKNTKAKSNPNLVQLQAKEIFKLGENTFIHPKRENIRIAMPRVLNMWV
                     MAPFFNSYFLNLGLKKENIIWSSETNEKMAKDGMKYGSIDPCFPAKVVQAHIHNLIYH
                     KEMDYIYFPAVTHIPNFLENVMASTSCPVVQGTPLVMKASFTTEKDYFNERKIQYVTN
                     ALNFEDLKYLKKQIFETWEKILNVTRKESDEAVDKSMNELRNFELRMQEQGRKILEEA
                     EANLSMAVLLIGRPYHLDPGLNHHILDEIQSLGFPVLTMTSIPKEKEWLQKYFDGNPF
                     DIRDVWPENFSANSAQKVWAAKFACRNKHIAVVDISSFKCGHDAPTYGIIDSMVSASK
                     AAYLTLHDLDQTAPTESFKIRLKTYAYNLNKRIEKMRLENEKWQQQLNSQKIQLHIGQ
                     TKTQENLAMPIAAL"
     gene            25989..27671
                     /gene="ilvD"
                     /locus_tag="PLF66_13405"
     CDS             25989..27671
                     /gene="ilvD"
                     /locus_tag="PLF66_13405"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016753122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HMZ65090.1"
                     /translation="MAEDLKRRSKMTTEGDNRAPNRAMLRAVGFVDEDFKKPMIGVAS
                     TWSEITPCNSHINKLAEKVKEGIRRAGGMPQIFNTITVSDGISMGHEGMHYSLPSREV
                     IADSIEVVTNAMRFDGVVAIGGCDKNMPGCLMALARLDIPSIFVYGGSIMPGTCDGHD
                     VDIVSVFEAVGKFNAGTMTRDEFLRVEQSAIPGAGSCGGMYTANTMSSAIEALGMSLP
                     GSASMPAVSSRKANDCYAAGIAIQELIKKNITPRQILTKKAFENAIRVVLVLGGSTNA
                     VLHLIAIAKEMGVGLSLEDFDRISKVTPHLADLKPGGKYAMFDLDKAGGVHGVMKQML
                     ADGMLHGDCLTVTGKTIAENLADMPALAKEQIIVRSKENPLFASGPLVILKGNLAPDG
                     AVAKISGLNKFAITGPAKVFDSEDTCFEAIMADKIKKGDVIIIRYEGPKGGPGMREML
                     AVTAAIIGKGLGEDVGLMTDGRFSGGTHGLVVGHITPEAFDGGPIAIVKEGDIVTIDA
                     TKNLLEVKLSQEEIATRLKAWKKPEPRYKSGVLAKYAHLVQSASNGAITNLL"
     gene            27939..28667
                     /locus_tag="PLF66_13410"
     CDS             27939..28667
                     /locus_tag="PLF66_13410"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HMZ65091.1"
                     /translation="MKEFLVIIPVYNEKATVLDVAIKTISACIDFADILFVNDGSSDG
                     TEEVLNSIQKEHPYLFVHHKKNGGYGSSLIYGFEFGANRNYPYLITMDCDEQHQPKDL
                     ARFANFNRKIDVVSGSRYAPKSRSIGIVPPEDRVEINRRITKAINKKYGWYITDAFCG
                     FKRYSTKKIKDYKFEENGYAFPMEFWAYAYKNNLSLEEIAVDKIYITDDRSFGEDLDK
                     KRKRYKYYLEAWRIAERKLKDYHR"
     gene            complement(28720..28920)
                     /locus_tag="PLF66_13415"
     CDS             complement(28720..28920)
                     /locus_tag="PLF66_13415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65092.1"
                     /translation="MNNVLLDTDILIDSSRKKESAISFLEKLEKDFVLSISYITKTTK
                     IINLLKNFPFYLTKKSNPPTNL"
     gene            complement(29170..29730)
                     /locus_tag="PLF66_13420"
     CDS             complement(29170..29730)
                     /locus_tag="PLF66_13420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65093.1"
                     /translation="MSEQKFIEEDLEFAFDGWEEIIQFDKTPDYKKLSDTLPNSKGVD
                     FLGVYRNGVYFIEVKDFRAHRIENQYRLAEKGTNLMIEVGQKVKSSLACISGGVRNST
                     NHEKFWKSIAQKILEKDLKVFIILWLEQDEALIKNSTMQARYKANISSYIQTLKTKFV
                     WFTTKVDIRNKPVGASDMGIKVEYLR"
     gene            complement(29723..30793)
                     /locus_tag="PLF66_13425"
     CDS             complement(29723..30793)
                     /locus_tag="PLF66_13425"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HMZ65094.1"
                     /translation="MNYKFSDLSINNITKFEHESLKFVPGINVIIGKNGTGKTHLLKI
                     LYTIAKSSSLANKDKLSFNERINNRIKGIFKIESNGKLIRNFEDKDFTINYKIFDEDK
                     DFFIQYYAFDQKLVSGFDQNITSIPIEHTFNVTYFPTQEMITAYSEFIPFYEKYDVKY
                     DETLYFLAKALSEPVTREQNEFQKKILAVIRDQLEGTLIKKGNTFYIKKETGEIEASM
                     LAEGMKKLATLLYLIENGTITENSILIWDEPEIYFNPKLISVLTLILNHLAMSGIQIF
                     MATHDYLLSHQLSLMDEYRSSYSEEIAEIRFFCLEESEKEGVKVHTGKNLTEIQNNPI
                     LEEFANHYDKERELFQRSMKDE"
     gene            complement(30807..32714)
                     /locus_tag="PLF66_13430"
     CDS             complement(30807..32714)
                     /locus_tag="PLF66_13430"
                     /inference="COORDINATES: protein motif:HMM:NF022062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIPR family protein"
                     /protein_id="HMZ65095.1"
                     /translation="MLEIVNNYETFKNEWLAPVLQKGKNPERTGSEFGRKLFSQWFEI
                     DEDSADFFEFNNHYTLGVDLAYYHEGSDEEENSNKEFVWYLIYSDLGDALKNLDSFES
                     YYDRLIQTIKNQKFITKEDLPFQQNLKSFLTKASKANEKLSYKIAIVLALSDEQKWDD
                     YDFVRQYIHRKEQPDSINIDVEIISIRTIYNRILEERNRTDIFDIKANLVESGSDLLV
                     GSIKLIDLYNFLKVYKKEKKDLDLIYQKNVRKYLGSRNKVNSDIRRTIIENPESFGLY
                     NNGVTIVAADYDYKKGEETNLIEPYIVNGCQTSKTIWATLEYEFANNTKNPHFHDWEK
                     KLNSSTVVLKIVKIGDITEDKNSAELLKSITRFTNSQNAVKATDFISLEESFNRFHAE
                     MADKYNIYLEIQRGGWDSRRALQKLNPTILPFFRDDDYANAFELIKVFASGWLKRPAL
                     AYGKNPPFAPGGSIFKQITDKADKNYINIEDLYASFLLQKEANFHKFGSVAELQSRKQ
                     TRHFFYFIVIEILRDIIYKAFNKPTNEKEIITKSLIQILKNESAKSELIENAALPILE
                     DYMTETKEHSIFREEEYKNDLNSFLKNDKWVGTTSNFKFLVSVQLSTMKRAYRGVIPY
                     NLLLDEVKKVG"
BASE COUNT        10349 a         5747 c         5689 g        11370 t
ORIGIN      
        1 gagtttacct ctgactatca atcgaagaaa acacttccgc aagttctgat ttatgtatca
       61 ttggattcgt ttgaatttga tctactgacg gatatttatc gaacggattt gatgaattca
      121 ggagatccat atgaagttgt agtctatgtt gcggaaacgg gagatttgga gaagttattt
      181 tcggaaatgt ttaatctgag tatgttctct cctcaaaagc ttttgattat aaagtctgga
      241 tctgactttt tcaaaggaat actcacagca ggtaaaaaag aactctacga aagttttaga
      301 cggaacatgc ctaatttagg ggatagagtc tacgtattgg ttcattacga tgcaaaggat
      361 attcctgcta aattgggcaa tttattcgac aatcgttttg gcttaattaa aaataggagc
      421 ttttatgcag aagagagaag acctgctttg gaagctatat taaaaacaga aaaagtgcaa
      481 ctagaagagc atgcgaaaga tgagcttatc catagaatcc ctccaaatac aggagcctat
      541 atcaaagcaa tccgtaagct caagctgatg ttaaataaaa aagagttttc ccttgcagat
      601 gtagaggaag tattatttaa caggtctgag ttcaatccct ttcaattggt ggatttactc
      661 tttagcaata ataaagcaga gttttttagg gagctttcca aattgaaacc gagtgcggaa
      721 agtaactttg tatttttatc ccttttgacg gcgcttctca atcgaaccga tgaagttcga
      781 aaagcaaagg ttctattcaa gagatttaaa gacgagaatg attccgaatt ttttaagtat
      841 ttaggtatgg cttcttattc ggatgggagg aagaaattca taaaatctag gctgagaaga
      901 gaaacaaggc tcttttccac tcgagctcta gatactctct atactttgat tctatcattc
      961 aataaaaagg caaagacaag ctcagtcaaa gatgataaca attattctgt gattcgggag
     1021 tttgataaat tattcgattt attagcatcg agttgacagg atttttggcc attttattat
     1081 gtcccttgcc cctcagggta atctcttttt ttttatatct aagagataga accaattgcc
     1141 gatttttatt gtatggagaa gaatttgatt caagagttaa aacctttatc taatgcccat
     1201 tttattccat cttctaaatc ctcatattct aactcagatt tattccatat gcataagact
     1261 ttggtgcaat gcgctgaaag ttttaatgaa tacctcacaa agtccaaaga accagttcga
     1321 ttcacggaca atgtcgttcc gcttaaaaaa gaatttttaa aatttatcca aacccaagaa
     1381 aaaagcactc tctttgatga ctttttaaat ttttaaccca gcgaaaattt cccgtaagaa
     1441 ttttttagcc acctacgaag ccattgatcg agaatgcttg gatcgaatcg aagggaggaa
     1501 cttggtatcg caaaaaagca tgacccgaaa actcttccta ctcagcggtt ttaaggtaaa
     1561 tagacttggt gaaatattaa tttgtaaaaa atcgaaacga tgagttcgag tcgctcgaca
     1621 agttcgatat aaattagcca ttttagtttt caaccctgct caaactgaaa tccgattttt
     1681 aaaaactcta ttttctaaag gctaaacctt taaacaagcc tcctgttaaa aaaaggaatg
     1741 aatctttgtc tttttttaaa agtatggaag tgtgaaaaaa aggttctatg atctacctcg
     1801 taaacttaag gcacctctca gatctcattc tgctcctctt tcttggaaat atgcaatctg
     1861 gagttttatt tctgggacag tctctatcct tgcaatcctt tgggtaacac aaattacagg
     1921 ttatccgctc ttgattggat cttttggtgc aacggctgtt ttactctttg gagtgcctga
     1981 ctctgctttg acacaaccaa ggaatttaat cggcggacat tttatttctg cagtgattgc
     2041 aattcttcta gttcaatttt ttggttctgg tttttgggtt acaggtctct cggttggatt
     2101 gtcaattttt gctatgtatt taacaagaac gttacatcct cctggtggtg cgactgcttt
     2161 gattggagtg atgggaaact gcactcctat gtttctattt gttccagtgc tcgcgggagt
     2221 tctgatttta tttttcattg ctttatttac aaataatttt gtttatcata gacaatatcc
     2281 aaagcattgg ttgtagattt agagtgattc attcaaatga tggcggctaa ttagagttag
     2341 ctactatctt aacctggttt agattctcag tggagacata ctcgtcttta ttaaattatt
     2401 ttttatatgt agagtgtaaa aaagtgattt ttacttctta agtctgcttt gaatttttct
     2461 tcgaaatgct tcccagtcat agctaatatg gagtgcttct agctctttgg tggagccata
     2521 gataagaaaa tcaagaattt cctgttttga cataccaata ttttctgata aatcggctat
     2581 gactcgatcc attcttcgcg agtctttttc gtttaagctg agttctaagg aaattcttga
     2641 ttcgaaggca gttaattcca attataaagt cctttaattt gtgatatagg gaggaagatt
     2701 gatttcattg gaaaattcta tacctagata catcatatcc tcatttctaa attcttttgt
     2761 taaataccat gccattttac ctgtagctgt ggcaaatttt tctaaactac catcctcttt
     2821 ttttttcaaa aaaacaaatg gatattgctt ttccctctgc acgaatactg ttttatctaa
     2881 aattatacaa gctccattgg cagaaatatc atttaagaaa caattgactg tttttcctgg
     2941 attggtctct aattgaacga taaaattatc tgagtccttt atataaaatc tcttactctt
     3001 tcgaacttgc tgtgacatat aacctcggtt gttcttcctt ttaatctagg tgtgttacgc
     3061 ctatactaat ttgatttaaa atcttttcct attgcttcca taaaaaggag aaagtttcta
     3121 ataaaaacaa aaaaagcaaa gtagtttttt tcttttcatt gcataaaaaa ataatttcct
     3181 tatgtctcat gttaattttt ataaaaaaaa ataacacaaa atgtagccct ttttttaaat
     3241 ggcaaagaga gcttttatat ctctgcattt ttaaaagaac catgtgattc taacgttatt
     3301 cttttaagag tcaatttgag atattttaat aatcaaaaaa aattagcgaa tcaattcgct
     3361 agaacattta ggaaaggaat actttgtcaa actggaactt tttaaaacaa aaaatagaga
     3421 atgatgattt tatcatcgat tgcagaaatc aggcagcata tgaagaaagc acgatcaagg
     3481 gagcctatta ttttcctttc atcaaaaaag catttggctc agatgccgaa tcacaaaaga
     3541 aaatgtataa tccgctcaag acaattctga atctagttca agaagcaaac aaatcgagag
     3601 tgatcgtatt tgatgaaggc atgggaatgt tttctgctag aatggtttat ctactacgtg
     3661 caatcggatt tagagaagca tatatgctag ctcttaaatg gccctttgag ggagatacag
     3721 aaccaggtaa gcatataatc gaagctgaac caaccgaaaa agcaaaaccg ttaaatgggg
     3781 ttgtggataa agcatttatg gaaaagaatc tcactcgtct acaaatcttt gatactcgaa
     3841 ccaaagagga atacgatgga aagcttccaa gacttacaag ccctgaacct ggaacgttat
     3901 ctggtagact acccggtgct tttttatggg attggcaaac attttatgac caagaaggtt
     3961 ttgttattag ccgtgatata tttataaaga gattacaggg attcccattt atgccagaga
     4021 gaacgacagt tctttatgat tataatggag ctagatcttg tttgtgtgca cttatgctaa
     4081 aagaagttgg ttatcaagat gtgcatacat accaagggtc ttggtttgaa tggagaaaat
     4141 caaaccttcc taaacaggcc gtttctctgt atggggcaag cgcagcgtca gccgcagctc
     4201 cacgtgttgg tggaattgac aaaaagaaat aataaactag gagtttataa tgataaaaaa
     4261 aatactatta ggtataatca tatcttttta ctttcagtgc tcgatgggac aaccttcaca
     4321 agttcgagtt ctaaaggaag taaacaccaa gggtgagatt ggatcggata tacaatatgc
     4381 aattgaagat aattcacttc gtgtcttcaa cagaaaattt tcttttgttg aatttccaac
     4441 agatcgctct tttttaacaa ttgcaaaggg ctctccaacc gttaaatacc acttagccgc
     4501 gtttaaattc aaagataatt ttacagctac ttgccgtaat caatcgatag acgatataga
     4561 aaagatagaa aatggaatta aaatttcagg tatcttacat gataaagatt gctatgtaaa
     4621 atatgatata gttctaaaag cagaaaacag tcacacttta aattttgatg tgaacatttc
     4681 tgataaaagt tacaacagaa ttaatcttgt ctatcgttct acaaaggatg agcaattttt
     4741 tggatttggt gaacagtata cacactttaa tatgaaagga aaaaatcctt ttattttcac
     4801 tgaagagcaa ggaattggaa gaggcgatca accaattaca tttgcagcaa atcttacgga
     4861 aggtgctggt ggaaatgagt atacaagcta tgctccaatg cctcattata tcactacaaa
     4921 tcatagatca gtttactttg aaaacagtgc ttactctaaa ttcaatttac agaatcctaa
     4981 ttttgtaaaa gttctttttt gggaaaataa tttaaaagga actttttggg tatcagataa
     5041 acctttagaa ttaatcgaat attacactgc aaaaactgga cgttatccgg cgttgccaga
     5101 ttgggcatac ggaacttgga ttggacttca aggtggaaca ggaaaagtta agaaagttgt
     5161 tgatcatgcg ttagccgcag ggaatccaat tacagcagta tggattcaag attgggtggg
     5221 aagacgtgtg actggttttg gagatcagct caagtggaga tggtatgctc aggaagaatc
     5281 tgaattaaaa gattcaaacg gaaacccaga accatcttat ccagatttta agaatttcac
     5341 agcagagatg aaccgaaaag gcgtgaaagt tctaggttat atcaattcgt ttttagcaga
     5401 tgcaaatcct aaaaatagtc gtaagcaacc aatcaaagaa ccaaactctt gtggatataa
     5461 actttttatt cctttttatg gatgcaatcc agaaagagag cctgattctt ttacaaaccc
     5521 aatgctagag gaagcaaaag caaaaggcta tctagttaaa aaccaagctg gagaggatta
     5581 tataattgag tcagtaggat ttcctgccta cttaattgat ttaaccaatc cagaagcagt
     5641 gaaatggaca aaaaatatta tcaagaaaaa catgatcgat caaggtctat caggttggat
     5701 ggccgatttt ggagaatggt tgccgtttga tgcaaagctc tacagtggaa tttctgctga
     5761 actttatcac aatatttatc cagtagattg ggcaagaatc aatagagaag caattcaaga
     5821 agccggtaaa gagggcgaaa ttgttttctt tacaagagcg gggtatacgg gaagtaaccg
     5881 ctactcaaca gctttctggt taggtgacca aatggtaagc tttggaacta acgatggtct
     5941 agcttctact gtagttggat taaattctgg tggaatcagt gggattgcaa tcaatcacag
     6001 tgatatcggt ggatacacag gaattaaaaa tcctccatgg tattttccaa attatgatag
     6061 aacagaagaa ctaaatagaa gatggattga gttaaatgca tttactccaa tttatagaac
     6121 tcatgaagga aatgttccct ctcaatttaa ccaagtttat tctgatgttc agatgactag
     6181 aatctttgct cgttttggaa agattcacta tgcactgaaa gactacttta ccttccttgt
     6241 tgcggaagca aaagaaaaag gatatcctgt cattcgtcat ccttatttga actatccatc
     6301 tgatccaaat acctatgatt taaaacatca gtttatggtt ggagaagatt tattagttct
     6361 accagttgta gaaaaaggag aaactactgt gactggatat tttccaaaag gaaaatggaa
     6421 acatgctttt actggtaaaa caattatcgg tggaagaacc ttcacagtcc cagcacctct
     6481 cggtgaacca gccgtttatg taaaagaggg tggggcttgg tcggatcgtg tatttacaag
     6541 catccaaggc gcactaaaat agtctaacgt tagttcgatg gagcaaaaaa tttaaaaaat
     6601 taccaccgag gacaccgata actaaacgat agagaaacga ttgataattt tgaattatcg
     6661 tatctcttat cggtgttcag ttcacccttc gctataagta ttcgcgaccg atgccttcgc
     6721 atgttcatca cagttcatcg gtggtaaaaa atcttacatt gaaattacgt tactctatct
     6781 tcaccaattt ataatcagaa ttggttacta tctattttct attttgactt tctactctca
     6841 acctctatta agacttagac ctgtgactaa cgttaaagaa ttcttttctt tctattaatt
     6901 gactcttcta aatgcaatca tcgcaatatc atcatttaat tcagagggac ctgtgcagaa
     6961 aatctttttg cctgaaaaga tttctacttg ttttgcaatt tcatttgtga ctttctcggg
     7021 cgattctttt ccatatttca aaatgatttc ttccatccta tcagaaccga ataactctga
     7081 tttgccgttc attgcctcat tgactccatc cgtatattga aaaaataaat ctcctttttc
     7141 taattggata ctttcttctt caatggttgt atcaaagata tcgttatcaa ttgcaccgag
     7201 tggcatccca ccggagggaa gagattctac tttattggaa attgctctat agagaaaagg
     7261 tttagagtga ccgcagttca tataatttac ttttcctgtt tttttatcat aaatacccag
     7321 aaagaaagtt acaaaaatat gagaaggtgt atctgcatag atttgttcat tcatagaaag
     7381 taaaattttt cgtagatctg tttcgccatt tcgaactaaa gattgaagct gagaacgaag
     7441 ggctaccatt acaagagcag gaccgattcc atgattggaa acatctccga tacaaaatac
     7501 gattttgtct tctcccgaat tcataaaatc aaaatagtct cctccaacac cggacataga
     7561 tttataaaaa tttccaaatt ctaaaattcc attcgcgtta gacggcatct ctaccggaag
     7621 caaacctttt tgaatttctt ctgccgccgc atattcaatg agcatttcct gacgatgttt
     7681 taaatctact gtcatttgat tgagtgctgt tgttaaaact ccaaactcat cggaaccacc
     7741 atgttgaaac tttgtatcta aatttccttg tcctagttca gcagcggagt gaatgatcct
     7801 ttttattttt cgaaccattc gatctgataa gtataaagct aaaaagaaag caactacacc
     7861 aataaaataa gatgtataaa gaattttatt tttttgctct tcaattcgat tgtatccttc
     7921 ttgcatatca atgactgttc tagtgaatcc aatttgattc gctgcttgta attctttggc
     7981 tagatttttc tcatcttcag aatagattgg gtttgcatct agttcccaca tgagctcctc
     8041 tgcttctgat ctacttttgg aaatcgttcc ttctgtgatc atttttttta gatcggctgg
     8101 aactgtttct gaattagcat ccatgatcca ctgacgcatt tttttccatt tgatttttgc
     8161 ctcttgacgg agcttttcat tggatgcata ttctaaatac gctgtaggat tcgatttata
     8221 tttcaggata atgtcttcat ataaagctac atctctcaaa tttaaaattg catttttgat
     8281 atgtaaggac tgttcattgg aaaaatctaa atttgttttc tctaaattct ctaggttcgg
     8341 aattttcttt gtgatctcat ttactagagc ttccctcttt tgtaaattct gtgcatactc
     8401 aatgtagagt tttctaaatt ctaaatctct agacggagat actcttgtct tatctgcttt
     8461 taaaacttca attctagctt ttagcttatc tgaaatttct ttaaacttag tttggatttc
     8521 cttttccttt tcaaagtatt ctttccattc gctagttaga tatttagaat ttaatttttc
     8581 tgctgtttca actgtaagat gattcttgta aaagagatca gattgtgtct ggtagttttt
     8641 ttgatctaaa ataaatttat ctttagaagt tgcagataaa tcttgttttg cgaggattcc
     8701 aacttgctgg ttaaatccat tttgcaaata atcatcaata gatttagaat tcacttctcc
     8761 ttttgtttca aagatattag tatcgaatcc aatctcagct tctacttggt ctccttgcga
     8821 gagtaaagaa aaagattgaa ttcgaatctt tgtcgttgga agtccaagct ctttgatttt
     8881 tcttttttgt tcttcttgaa attgatttaa aatctgtttt ctaagttgtg ctttttgtgt
     8941 agagaggttt tttcgattag ccgctatgac agagtcagcc gctttgtttt ctagattttt
     9001 ttccagtaat gattctgctt ttacaacttg atttgcatat ctttgtaagt ccgaaaatac
     9061 tttatctgaa attggttttc cgtctttatc tttgaattga gtttttatat ttgcttctaa
     9121 ttctttaatg tctttctcgg tataataaat cgaaaagaat gtatcaattt ttcgagctga
     9181 ttttttagat tgaatcaaac ctagtttttt tagaccaaag tcgcgattga ttttgaataa
     9241 acccataaag cttttttctt ctacaaaata ggttttcttt aaagcagcta acgctttttg
     9301 attctctttg atttgatctc tgaaaccttc aataagaatt aaacttctag aaattccttc
     9361 taagtcagaa actacatttt caatataaga ctttgaggca gtgatttctt tttcataact
     9421 ttcttccaat gtcatttttt gttgcctgat aaaaagttgt gacattacaa aaatgatgat
     9481 acctacaaag aggatggtga ataaggataa ttttgcgcga atcccgggta atatgattct
     9541 ccaactaaga tttttcaaat tcagtctcct ctgtgaaatt tttggtaagc gttaaacggt
     9601 tttttaaatg aaccctttcg tattctacat tcataatttt ttggacaatg tgaatcccca
     9661 ttcccccatc ttctcctaat tctaaatgct catctacatc gacagggggt ttagtcaaag
     9721 gattgaaggg gatgccttca tcttccaaaa caaaagtaat tttatctttc tccaatcgag
     9781 cataaacaga tatcaaagaa tcttttactt caggaaagcc atgctctata atattagaaa
     9841 tagcctcatc tatagaaaga atgattctat ttttaaggat attgtctaga tctgatccta
     9901 taaaatcatt aattttcgtt cgaacattag aaagaataga aagttgattt ggaaattgaa
     9961 aattgatttc tctcaaaatc catccaaggc tttttctaaa tcaggaaata ctttgaatgc
    10021 tttagaaaat cccattacat caaatgcatc ttttacccca gcttgaaaag aacaaagtct
    10081 aagttctcct gattttttac gactggcagt aagaccggca agtaaagcac ccgcgcctgc
    10141 agaggaaaga taactaacgt ctttcatatt gactacaatc tttgtttttc cagctgcaat
    10201 tccagtttcc aattttgttt taattgctgg agatgtatgc gcatccattg ctccattaag
    10261 cgttagtatc caaatattct ttgattctga ttctgttatt tccattattt cctctttata
    10321 atattatttg cgtagttgtt tccataagtc tttttttgtg atgactgctt gtagtttgcg
    10381 ttctaattca tcaatttcca tgaagtattt gatgtcagga tttccttgga aaataaatgc
    10441 atactttcga atggcttgga ttgctttttt taaatcgcct tctgagattc cactaaagat
    10501 agaataatat aaattgatcg caataaaatc atttagttct gaagtaaatc caggagagcg
    10561 actttctagt gcttcgatta ctttttctac ttcgtctcga ttgttgtctt cttgttctgc
    10621 atattgaatt aaataatttg caatcaattt tctacatcta tgaatgtcac ctttgttact
    10681 gtaaatggaa atttggttga atagtgccaa tgcttctttt tgaattttta cgatgacttc
    10741 ttttttcccg agttctactg gatttaacgc atcagtaagt tcttgattgg aaatattttc
    10801 tagtttgtct tcaaaagatt tttctttttt agtttgattg tctaaattga attcggtgag
    10861 tggatctttg atgatcgtaa caaattccac tccttcttcg cttttagcta ggacaggaat
    10921 agagcccatt ctatctaaga aaaaaatact ggaattacca agacttgaag cagactcaag
    10981 caaggactgg gaaatcaatc ttttgcagag aggaccaatt gtgttgagtt ttgcaaggtc
    11041 agacctagct tcatcgattc ttgccatgat gaatttagaa tcattatatt tcatctgacg
    11101 ttcccatcca gcataaacca gtggttggta taaatgccta tgaaaagaac cttttcccgc
    11161 tttagcaaga agtcgaatgt atctgcttaa ttcttccgct agtgttggtg tgacttttga
    11221 aattttttgg gtatccacaa taatctccct gcttaataga atccatttgg atgaaattga
    11281 taagaatatg attctagtag atttaaaata gtcaagatga ctttttcttc tcagtttatt
    11341 tcttttttga gagttatagc cattttaatg attaccacag attcacacgg atgaacaccg
    11401 atgtttgagt ttcattcatt cgttctatga tttttacttc tgattttctt aattcttatc
    11461 tactaggctc ataatagata agaatagcac cactgccaaa tgttttggtt tttgtgagtt
    11521 ttagaagtgt cctatcgttt atatgatcaa ataaaggaag accttttcct atcacaacag
    11581 ggtgaatgca aatctgcaat tcatcgagta agccaagatt taggagttgt ataaataaac
    11641 tgcgactgcc aactagaata tcttttcccg attgttgttt tagttctaat acagtctctt
    11701 ttaaatcacg gtttgcgagt cttgccgatt tccaatctat gcttttaaga gaatgagaaa
    11761 aaataatttt tggaattcta tcaattgcag ttgcaaactc attcataaat ttttcatctg
    11821 aaggattttc tataaatggt ttccaaaatt ccattaattc atacgttatt cgcccatata
    11881 aaatagcatc accctgtcct aataaatcag tatagtgttg atgtatttct tcatccggta
    11941 tccctgctgt atgatcgcaa aatccatcca gtgtcatgtt cagtgctgca attatttttc
    12001 tcattttaat tctccatcac catttattta tataatattt ttatccgacc ttgtcaagcc
    12061 cactaggtta ctagaatttt tttaaaagaa ttatttactc accttacgca aggtgaacca
    12121 agcgttcaaa tgaggtttgc tcctagcgga gggcttgccg ccgcaggcta ttgattttat
    12181 tagtttatta gcatttccta atattattaa aatcatttct ttttccttaa aatgtcaatt
    12241 ttgcgtaagg tgagttattt attatttccg cttcccagcg gggggttcat actttttctt
    12301 ttctttatgt aaagaaaaag tatgcaaaaa gaaaagaaac ccgaaactgc ggctaatcgc
    12361 tataacacgc aggattctcc attggggctt tttctatttc ggtttgttgg tgattttttt
    12421 tatatgcaat agcaattcta aaaatttatc ttccgaagct atagaaaaag ctttagaaga
    12481 ataattactt ttagtaaatt aattttattt agatattcgt caatttgctt tcgggtctac
    12541 tttctagtta tgaaatacca gattgagtaa tggttttaaa tttctgttgt atattttaaa
    12601 aatgttttct ggaaaaccta tttttgtaag tcctttcatt cacttctaga atagtattca
    12661 aagtgaaaag gtaaataaaa acaggttgct gaattatcta aatatattta atataaaaaa
    12721 ataggcactt atctatgcag aacaatgtta cattgaagca gaatttaaag gtaaacgata
    12781 actttattta ttccttttgt caaaaatgga agattgatga attttctttt tttggatctg
    12841 ttttgcgaaa tgatttttct actgaaagtg atattgatgt aatggttgca ttttcacaag
    12901 attcaagatg gtccctcttt gatttaatgg atatgcgaga agagctagaa aaccaattca
    12961 atcgaaaagt ggatttaatt aataagaaag ccgttgagag aagtaaaaat ccatatcgta
    13021 aaaataatat tctgaactct tatgaggtag tttatgcaaa aaaatgattt atcgcatgtt
    13081 atggatatat tcaatgcatg caataatatt atagcctttt caaaaggaat ttctcatgag
    13141 gagtttgaag aagatatcaa aactcaatct gcgattctgc atcaatttat gattatgggc
    13201 gaggctacaa agcgtttaac ggaagagttt aaaagtgagc acaactctat accatggcga
    13261 aaaattgccg gttttagaga tatactaatt caccaatatg ataacattat accagatgaa
    13321 gtttggagaa ttatagagac tagcgttcca gatttggcaa gagacttagc tactttatta
    13381 atttcgtagg gaataagagc cctttttcat ttcctctagt ctaggcgtct atcgatctct
    13441 actttatatg ctttttctat ttcagctttt tcccgattgt tttctattta gccaatctta
    13501 atctagctta tccagtgcat ctggttcgac tgcggggact ttttctaata tagattgaaa
    13561 atgggaccaa cttcctctct tggcacgact atcaatattt tcctgcatcg taataattgg
    13621 gagtagtgct gagattgcca ttgagactat ttggtctata ctgatatttt cttttcttgc
    13681 aatattttct atttgcgtaa acagaaaatc aggaatttgg ctttggatcg ttttcattga
    13741 atccctccta ataattgtag aaactctttc ggagtaatag tttttatatt catctttttc
    13801 gcatttaaaa aattagagac attgaatgta ataatataat ctatattaga tgcaatagct
    13861 aaatcaacaa taaaatcatc gtctgggtca gatgttgtag gtcgccacaa gtaaaatata
    13921 ttttttgtgg atgcaattgc gcaaatatca tctagtaaac tgtcgatttc attcagatct
    13981 aagtttacca aatgattgtt tctttttaaa atctcttcat attcaaaaag cagagaatta
    14041 gacagaaata attgaattct ttcatcattt aaagatgata ataatttata ggaagccccc
    14101 aatctagatt tcaatccgga aaccaataca tttgtatcaa ttataatttt atatttcttc
    14161 atgtgcgaat gccttccatg cttatgatag acttaatctc atcttccgtc aaaaccttaa
    14221 attcacctgt atcaaaatat tctatatcaa tgtcatctgg aaataaatct ttctcttttg
    14281 caagtaaggt tttcaattca atttgaatcc tcttattcaa ctcaatcctc taagatctaa
    14341 tttatttcga tcttcattaa tatgctacta agaatttaat attcgttcgc ctgtgttctc
    14401 ttatgctttt tccggttcat tctgaatgat ttaaaaaaga attcacttcc atttcaatcg
    14461 ccaatgctct ttgtattacc tctgtcagca ctgtgatttt tttgattttt atgattcgtg
    14521 tgattcttta ataagtaaaa attttaccta taacaaaatc atggtaatca catgaatcat
    14581 aaaatcctta gttcagacag tgtttacact tcataaaaaa acaaagataa aatcaaatat
    14641 ccaaacaccc caactccaaa tcccatcggt gtaatcggtg gtaatcttct aatccctacc
    14701 caacaacaag ccgtgtgaac ttctctgcaa gagcgatagt cgctgtagtg ccggagttga
    14761 attctaaaaa gtcagactcc attccatcgg agaagattac gccggactca ggcatgtggc
    14821 tttcgaggat aatattattt tgctcggaga tttcaccggc gacaagatcg gagccactcc
    14881 atttgctttt gaaaggctct cgcactacaa agatcattct tctttcgtcc cattccaaac
    14941 gagagggaga caaatcactg ttatcccctc gaaaatttgc gattccttta gccatgttga
    15001 agacagaact gagccaacct gtagagccag ccggagttga gacgatcaca ccgctagagc
    15061 tatgacgttc ttctcttcct ctgaacttga tagtataacg agcactgata tgagatctag
    15121 ctcctacaaa aaaatcattg aaggcaaaga gagattgacc atcgtttaac tgcactttgc
    15181 ccatcgtgat atttcgaatc cttggacttc ctttgaggag agaatgcatt gccttgggta
    15241 gagtggatat ttggaatgga agaaggatac catcgaaacg ctcaggatcg ggatttactg
    15301 caacgatggg ttgaccgttt agatattttg ctgtatttac aacaagacca tcttgtccta
    15361 gagtcatcac aatgtctttt ggaccgaata caaaatttgg taagaagctt ctatctatca
    15421 cttgtaattt tatatcggaa ggaattgcat gcgttacttg gtctcttgca tattgataat
    15481 tagaatcttc tttttcgtag tcgtcaaacg attgtcctcg cgattttaca aagaattttg
    15541 cctgtgcctt agtgctaaac ctttgcacac tttcttgtag tcttgtcgcc cgaatcacta
    15601 aaacaatctt ttcaatatcc ataatcttct aaaaggattc cccaatgctc atttgtcaag
    15661 taaacggttt attcaccacg aagggcacga agaacgcgaa gggtttgata aagactactt
    15721 ggagcgaaga gtgggatgaa caaataagga cttctctttt cttcgtgaat cttcgtgctc
    15781 ttcgtggttc attttttttt gcatctgaat accatcgttt tacatcgttt tgtaatcggt
    15841 gtcctcagtg gtaatctttg aaagtcttcc tatgctgggg atgcggaaat aataaaaaaa
    15901 aggattgttt tctaggtgtg aaattcaata gcttactaag aaccaagtct actgaatcat
    15961 tcagattcaa aggctagtga ggatatgaat ggagttttta gaaccctata caaaatacat
    16021 tctttttaat tttttttctt ttggaacatt actcgtaacg atctttactt ttgcctttgc
    16081 ttattttttt ctcaccttac ccaataaatc agaaagcact cttcatttag gtataggctt
    16141 tttatttttg gcttttttca atactggata tttttttgcc gcctcttact atcacccttt
    16201 tgccgcttat cacaggtggt ttactggagt ttttattctc cctgccatat tacacttcgg
    16261 acaatttttc tttaaatacc cgcgcgatac aaatcctaaa ctctccaaac ttgtctttag
    16321 aattatgtgg gcagttgcca ttctcggagc gattttattt ataagcggaa cttggaatgc
    16381 ggatcgaaaa tttcatttta cagggcatta ttgggatttt gatgcagaac caattagtag
    16441 aatgcttgcg gttctaattg caatttattc aatcatttct ttcatactca ttggtgcttg
    16501 gaaagtttat gtagttaaaa caagagaaag atgggtcatt ctaaagattt taattgcgat
    16561 gctcatcgct gccgtcactc ctaacgttac aaatattatg agtcgagatg gagcaatcga
    16621 aagatcagct tatttaatag cattgactct ctttttagtt ataggatttt ttatcatatc
    16681 gattgtttat attaattcca ccaaagatag aactaccttc atggtcaaga ttgtaggact
    16741 aacactggtt acactcttgc tcattttaca ggcggctaac ttctattcca tacgggataa
    16801 agaagtagat tatgatacat taaaatcaga aaacatgtct cgtatcatag agggtgggga
    16861 taaatcaaag gatactaagt atttcattca gctcaattta gatgaattgg aaattaaaaa
    16921 aaaagattat cctagttcga ttaacttaga tttgccttta gtcgaagttg attttagaaa
    16981 tacagtaatc tatgaggata taaaaaattt acctgaatct aattttagat ctgcactcaa
    17041 tgaatattta tttaatacgc atccttattt tgaaggattt aaaaatagca ttggagagta
    17101 tctagtcaga aataaagatt taggagatag tgatttaaaa gatgcaatct ttcctttttt
    17161 agatagctta aataaattta gtttcattca ctccaataaa atcagccaat tagatgaaaa
    17221 agatttttgc caagcaatta cgaaatattt aaatgcaaat gcgttaaaga attttaaaat
    17281 tgccattgag aaaaacataa atcaatgcaa aatcaatgga agacttttag attcagcaag
    17341 tcaccgaaaa gaaatcaata aatacttcag acattttcaa ccagccaata caagaagatt
    17401 cagaaaaagt atagatgaat atggcaatca aaaacactac atcgcttaca cacattatga
    17461 agtttataca caggtagtca gtgaagttgg ttattcttat ttaggatatc gacaatacat
    17521 gcacacatct gcttctaacc agcagtttat attgattcta gttgtttgta ttgtaattgg
    17581 ggtctatccg ttatttttta gaggaagtct tgttactcct ctttgggatt tagtccacgc
    17641 tttggaaaaa gtgaaccaag gatatctaga tgtagaggtt cctatcaaag taaatgatga
    17701 gatcggattt ttatctgatt catttaatgc gatggtgatt tcgattaagt atgcaaggtc
    17761 tgagttgcaa cactatgcag ataaactaga agacaaagtc caagaaagaa ctaaagaagt
    17821 gcaagagaaa atggacgagg tgcaaaaact caaagtgcag caggatggag attatttcct
    17881 aacgtcctta cttgctaagc ctctctttat gaatgcaaat aaatccgaaa tcacaaagac
    17941 tgaatttttt attaaacaaa agaagaattt tgagtttaag ggtaaacaag tagagatcgg
    18001 tggcgatatt tgtgtcacag gaaatttacg atttggaact aaattaaaac acacacgtta
    18061 tacgatggcg atgaacgcag atgcaatggg aaaatctatg caaggagcag gtggatcgat
    18121 cgttatgggt gtcgtgatga attctcttat ggcaagatcg gctgctaaag attgggttgt
    18181 cgacaaagct ccggaagtgt ggctaacgga tgtatattac gagattcacc gtgtctttaa
    18241 aacatttaac ggttccatgg taatttcttg cactgtcatg ctcatcaatg atgaaactgg
    18301 agaaacttat tattttaatg cagagcatcc ttattctgtt atttatcgaa atggaagagc
    18361 ggagtttatc gaagatagac tcaatttaag aaagcttgga ctcgattcag aaattccatt
    18421 tcaagttttc aaaatccaat tagagccagg ggatataatc ttgttagcct ctgatggtag
    18481 agatgatatt gatttaactc cagaagagcc tgtgcgaacg atcaacgaag atgatacttt
    18541 atttctaaaa catgtagtgg caggtagagc ggatatacaa gaaatctatc acagcctaca
    18601 aagatcggga gaatttatag atgacttctc tatacttcgt atcgcctatc gcgaagcagt
    18661 agttcattat gcgaatccac tagatgaacc aggaattata ctccctaaag atatcgatca
    18721 tgatgattta gaaagtgttt acagtgagtg taaaaaactt ttacgtgata ataaagatga
    18781 agcagctaga cagcttttac aaataactta caatcgttcg gaagcaaatc aaaacaattt
    18841 aaagataaac aagctctatg gattgctatc ttttaaagcg agagactatg tttctgctgt
    18901 agacgtgatt cgacgttatc tgaaagatga tccagcttat gaagagtttt ggtattatct
    18961 ttctatggca gagaaaaaac ttggacatta taatatggct atggaagcag gggagaagtt
    19021 gcgcgaaaga aatccagatc atactctcaa cctactcaac ctagcggatg tgaatcgatt
    19081 aatggggaaa agagatgcct ctatagcgct tacagagctt gtattgaaat tggagcctga
    19141 taatacgggt gcaaatcgac tcatgcaaat tctcacatcc attttatgat accagaattt
    19201 ccagaagata tagtttattt agaaaaaagt gatcggcatc ccaatctata tgatgtaagt
    19261 ctatctaata ttgataaact acgagattac gactcgatag aagtccatat tgttttatat
    19321 ccgtatagta gacgcataac gtcagaacat attagttttt ttccatttga agagtatgta
    19381 aaagatgtgc agtccaatca actttctgcg tattcgaaaa tacaattgaa gtttcataag
    19441 ttttttggag tagcacttgg ttctgtcatt gctggatttt tttataagta taaaccggaa
    19501 gatttatttt cagtagaatc tgttgtagct gtctttgctg cttatgcaat tggaaaagaa
    19561 ctatgggctg atttagaaag attttttatc aatatttcaa aacgctggag actacgttat
    19621 acggaagact actataagta tgagctagaa aaatttacaa cgatggctca ttattcttct
    19681 cttgcgaaga gaaagcgtta tggcaaggtc acacttttac cagagaaaat taatttctta
    19741 gaactcagta attcccaaac acttagaatg cattttaaca tgaaagacat taagcccgca
    19801 gaggcaaaga gcgctcatat tttttcaatt catatcgagc caagtttaca atctgatttt
    19861 gaaaaatatg gtcacttgtt tggagtaaaa ctcagtttga ataaaaaaac gttaggtttt
    19921 acaaagagac tagaactctt tcaatccctc cacaacaggg aaaagggctg tctagatgac
    19981 gaggaagttt ggtcggctaa ttccattttc tgtcgaaagg caatcacact cggtaaaatt
    20041 aaattcttcg tcggaagaga cttactctct ggcaaatccc tcgtgcgtta cgtggaacag
    20101 aagtttagaa aaaagaggta aaaaattaac caccgaggac accgataact gaacgatcta
    20161 gagacgacgg ataatttttg aattatcgta tcttttatcg gtgttcatct gtgctcatcg
    20221 gtggtaatct tttaatattt ctaccatata gaactgacgt ctatttcttc aattccccgc
    20281 tttcatttgg ttgattactt tgtgcattgc catttggatt ctagaataga aatttgccgc
    20341 accgtctcca tttgtttcta ctgcaatgaa ttctacttgt ggatataaat ctgaaattct
    20401 agattgcact ccatcggata cagcagagga actaagacaa gtaaaaggct ttacactgat
    20461 gattaaatct gccagtttgt gtttagagat atggatatga tgtgcgactt ccatatgatc
    20521 ttctccaccg tctacatgca gatcaaagtg ttcgtgggaa atgtttgcga gcatatcatg
    20581 atcagaaatt tttactgtct ctcctaaaac aagtcccatc attttaaaaa tcgtttttac
    20641 taatttctca acaagtaaaa ttttcatttc actcgaactc atccagaaag attcacgctt
    20701 tcttttttcc atccaacgag cacgccagag ttgatgcatg agccaatcaa ttaaagattc
    20761 actcgataca actccccctt cttgttcaat aaatctgtag aggttatagt ttccttctcc
    20821 gtctgtagtc tttgcccaaa attctcccat tatatgcact aacggtttct ttttggatgt
    20881 atctagtttc atacctttga tttgtttacg tgctttccaa agacctctcc acatttgttt
    20941 tttgcggata tgagtcttga tggtctgacg aacttctagc atcgttctat ctacagatcc
    21001 tttttcgatt tcaaaaggac gctttctaac attgactgca ttgagtagat ctccaatcat
    21061 gatggctcca aatcctcgaa gtaaatcgac attacccatc ttgagtcctt ccatgagaac
    21121 atcatccacg tctccagaac gcgttagtag taatactcta aaaccttcaa acccagaatc
    21181 acgaagagcc tttctgtatt ctgttgcata catccccatt cgacaaggac cacaacctcc
    21241 cgcagtgata aaaccataat tgtttatgat atcttctgta gaaattccct ctttatcgcg
    21301 catgtgaatc aattttttaa ttagattccc cactgtgaaa taggtcggat tgcattgacc
    21361 tctatttcca aattctctac ccagttgaag ggcttctgaa tttggttcct ctaagatttc
    21421 tagattgata ccagcgagtg ctacagcatc tactaataac gcatttgtat gagctggaaa
    21481 tccagttgta aatagagtta cagtgctgcg atcggcatgg ctaaattttc ttgggttttt
    21541 gtctgtccaa tgtgtaattg tattttctgt gagttcagct gttgctgcca tttttcattc
    21601 tccaatcgca ttttttcaat gcgtttattt aaattatatg cgtaagtttt taatctgatt
    21661 ttaaaagatt ctgttggtgc agtttgatct agatcgtgaa gcgttaggta tgccgccttt
    21721 gaagcagaaa ccatactgtc aatgattcca tacgtaggag catcgtgacc acatttaaag
    21781 ctagatatat ctactactgc gatatgctta tttctacaag caaacttcgc cgcccaaact
    21841 ttttgagcag agttagctga aaagttttct ggccaaacat ctctaatatc aaacggattt
    21901 ccatcgaaat atttttgtaa ccattctttc tcttttggaa tgctagtcat agttagaacc
    21961 ggaaatccaa gtgattggat ttcatctaag atatgatgat tgagtcctgg atctaaatga
    22021 taaggtctac caattaaaag aactgccatg gatagatttg cttcagcttc ttctaaaatt
    22081 tttctacctt gttcttgcat tctcagttca aagtttctga gttcattcat ggatttatct
    22141 actgcttcat cactttcttt tcgtgtaacg tttagaattt tttcccaagt ctcaaagatt
    22201 tgctttttta aatatttcaa gtcttcaaaa ttcaatgcat tcgtaacata ttggatcttt
    22261 ctttcattga aataatcctt ctctgttgta aaggatgctt tcattacaag tggagttcct
    22321 tgcacaacag gacaagaagt agaagccatt acattttcta gaaaatttgg gatatgagtg
    22381 actgcaggaa agtaaatata atccatttcc ttatgataaa tcaaattgtg aatatgggct
    22441 tgcacaactt tagcaggaaa gcatggatcg atagatccat atttcattcc atcttttgcc
    22501 atcttctcat ttgtttccga actccagatg atattttctt tttttaatcc taggtttaga
    22561 aagtaagaat taaaaaacgg agccattacc cacatgttta aaacacgagg catcgcaatt
    22621 ctaatattct ctctctttgg atgaataaag gtattttctc ctaacttaaa aatttctttt
    22681 gcttgtaatt gaactaaatt tggatttgat tttgcttttg tatttttctt tgccattaat
    22741 tcttttagtt cttctttgct ttcaactgtt cctttatcac atgagaatcc agaaatataa
    22801 cgcactttct gtccacttgg agtaagcgta tcgatgaaag ttctagagca agaattggaa
    22861 caaaatctac aacgagttga ctcatctgat ttagaagaat attgaatatc ataggcttca
    22921 tcgagtccgg caaactttgt gtagcctctc atcatcacag tctctcttgc ttctagagct
    22981 gcgcctatcg ctcccgcttc tcctggatgt ggatgaacaa atacttttgc attcttcaca
    23041 cgttttttaa tataatctac ctgtgctttt agtgcggcta gattgtattg agttcctcct
    23101 tgtaaaacaa atttgtttcc aaattgttct agtcttggaa cttgaaccac atattgccaa
    23161 atatttttag gaagaacctg tgcaagtcct gccatgattt cttcttttgc aaaaccttgc
    23221 ttttgaaagt tcactcgatc tgtatctaga aagactccac acccataaga aaatacagga
    23281 gctagttctg ctttgaatgc gatatccgca tactctcgca cagaatgacc gaactgttct
    23341 gccatacctt gcaagatcat tccattgcct gcactacatt gattggatag tttgaaattt
    23401 gaaagctctt gtgatttcat aaaaagaact tttatatctt gtccgcctat atcgcagata
    23461 acgtctatat ctccgaaata attttgagaa cttttcatgt gagctacagt ttcaattaca
    23521 ttcacatccg cagaaaaaac tttttcaaaa atattcgcag cgtatcctgt tacaccgaaa
    23581 ccgattacat cgagttgaac tcctttctta cttgtctttt ctctgatctt atcaattaaa
    23641 actttaaaat cttcgaacgg atttcctttg gaaagagtgt attctttgat aatgagctta
    23701 ccgtcttcat cgagtaacgc acatttcgta gaagtagaac caccgtctac tccgaggaag
    23761 gctctcactg tttgtcctgg ctgataattc ggagtagtga aatttggaat tgcatactct
    23821 tctttgaatt gtgtgagttc ttctgaatct tgaatcagtg gatctcccgc aatgtttttt
    23881 aattctgcct ttcttccacc cgaaataaat tcttctagtt ccttgtagcc tttgtattct
    23941 tctgaatgac tagaagctcc ataataacaa ctaccgagtg ccgcataata gagagaattc
    24001 tctggaacaa tcacttgctc ttcgatgttg ccttcataca aatcttgaaa tccacgttct
    24061 ttccaaatct gtttgattct atatttccag cattgaacta aaaatggaag atagtggttc
    24121 ggtccaccga gtaaaatgat tttatctttt aatgtatgac cgcgagtgag aacagaaagg
    24181 ttttgattta caatagcatc cgcaagcgaa ttcataatgt catgttgagc agttcctgat
    24241 ttgattaaat tcacaatatc agtttccgcg aatactccgc attttgcggc aacatggtat
    24301 aatttttctg gattgaattc tattttgggg agttccgacg gatccattcc cactttaatc
    24361 atacatttat caatggtggc tccggttcca gaagcacatt tatcattcat aaaagtaaga
    24421 attcgttttc cagttcctgg attctctttg aagataatta cctttgcatc ttgtccacct
    24481 aactcaatga cgctctctgc atctgggtga gacttttcca ctgccattgc aactgcattt
    24541 acttcttgaa taaaacgagc tccaattttg ggagcaaggg ttaccgcacc agaaccagtc
    24601 ataaatatgg aaacattacc tggtttaaaa gaaggaaagt tctcttctaa ggtttttaaa
    24661 aacgcaagga cacattgacc ttgttttgtg tggtgtcttt catatcgagt gaataagata
    24721 tttagattct tgtctgtgag aacaatttta acggtagtgg aacctacatc gattccaagt
    24781 aagtattcag agtttttatt tatcatttta tttccaatgg ttataaaagt ttttaacaaa
    24841 atcctagcag agaattttca ctttaatgta aatagtttgt tccctacaaa taaaaaccaa
    24901 tacaccaaat tagcaatatg tttaagtggt ttttgctaaa aacggactat gatctatatc
    24961 aatatttttt ttgaaaaaaa atgttttctt ggccaccaaa aattgtaggc agtataaatg
    25021 tgaattattt taaacaatgt tagttctaaa tcgataagta ggagatctat aggggataat
    25081 attgattagt cagtgatcca aagaaatcat ttagagatga aagatagaat cttttttcat
    25141 tctcccgttt cattttaaaa ataaaaggaa tttttaagaa acaaataaaa gcacgccttg
    25201 tggtatcact ttgaggagga agagtaaaaa aagtcatcgg caatagactc tcctttttca
    25261 ctttccttgg gtctttcctt gattttagat ttttttgaac aagaataatt agaaatctag
    25321 agagtaattt ggcttattct tttaggggat aattatgatt agtctctatt ttagaactca
    25381 tttggtttct aattttagtt tgaaataaaa tgagaatcta gatggatcaa gggaatgtaa
    25441 agttcaatct gcaccaatcc tctattatag ttatctcaat tattcattgg agaaagttat
    25501 aagattctat caatctgaat tgaatacaaa attactgtat ggagatttgg ggaatagatt
    25561 ggcgactatc gatataaaaa aatagaaacc tatttaaatc aatccttccc attctcagag
    25621 acttctcctt gtcctgttgg aaaaatttaa atctgatctg aatcgatttt gtagttttaa
    25681 aaccttgcaa agaaaattct tatctctgag aatacaaagg cttttaagtt gggtctcttt
    25741 ctggttttta caaatagaaa gcaattgaaa ccgaaatcct cgtataaaaa atacctttac
    25801 aatattggct tttctacaat ttactaaaag cattgctaag aactgtgtaa aagctgagca
    25861 aaagagtcgc agaggaatgc cttaccagag cattttgttt tcgaaaatat ttgcttttac
    25921 acaatctcta aaactaaatg tttatcatta aatgagcagg aaagaatttt tttaacaaag
    25981 gaaatacaat ggcagaagat ttaaaacgaa gaagtaagat gactacagag ggagacaata
    26041 gagcgccgaa cagagcaatg cttcgtgcag ttggatttgt agatgaagat tttaaaaaac
    26101 ctatgatcgg tgtcgcatct acttggtcgg agattactcc ttgtaattct catataaata
    26161 aattagctga aaaagtaaaa gaaggaattc gccgcgcggg tgggatgcct cagatattta
    26221 acacaattac agtatccgat ggaatttcaa tggggcatga gggaatgcat tattctctac
    26281 cttctcgaga agtgattgcg gattctatag aagtggtaac taacgcaatg cgttttgacg
    26341 gtgtggttgc aatcggtggt tgcgataaaa atatgcctgg ttgcctcatg gctttagcta
    26401 gacttgatat cccttctatc tttgtttatg gtggatcgat catgccagga acttgtgatg
    26461 gtcatgatgt ggatatagtg tctgtatttg aagcagttgg taaattcaat gctggcacaa
    26521 tgactcgaga tgaattttta cgagtagagc aatcggctat tccaggcgca ggaagctgtg
    26581 gcggaatgta tacagcgaac acaatgtcat ccgcgatcga agctctcggt atgagtcttc
    26641 ctggttctgc ctctatgcct gctgtaagtt ctcgtaaggc aaatgactgt tatgcggctg
    26701 gaattgcaat tcaagaatta atcaagaaaa atattacacc aagacaaatc ttaacaaaga
    26761 aagcatttga aaatgcaatt cgagttgtat tggtgttagg tggatcaacg aatgcagttc
    26821 ttcatttgat tgcgattgca aaagaaatgg gtgttggtct tagtttggaa gattttgata
    26881 gaatcagtaa agtcactcca catcttgcag acttaaagcc tggtggaaag tatgctatgt
    26941 ttgatttaga taaagcaggt ggggttcacg gtgttatgaa acaaatgtta gcagatggaa
    27001 tgctgcatgg agattgtcta actgtaacag gaaagacgat agctgaaaac ctagctgata
    27061 tgcctgcact tgcgaaagag caaatcattg ttcgttcaaa agaaaatcct ctttttgctt
    27121 ctggacctct tgttatactc aaaggaaatt tagctccaga cggagcggtt gcaaagattt
    27181 ctgggttgaa caaatttgca atcacaggac cagcaaaagt ttttgattca gaagatacat
    27241 gttttgaagc gattatggct gataagatta aaaaaggcga tgtaatcatt atccgctatg
    27301 aaggaccaaa aggtggacct ggtatgagag agatgcttgc tgttacagct gctattattg
    27361 gaaaaggtct tggggaagat gtagggctta tgacagacgg aagatttagt ggtggaacac
    27421 atgggcttgt agtcggacat attactccag aagcatttga tggcggacca attgcaattg
    27481 tgaaagaagg ggatattgta acgatagacg caactaagaa cttactcgaa gttaaacttt
    27541 cacaagaaga aattgctact agattaaaag cttggaaaaa accagaacca agatataagt
    27601 ctggtgtgct tgctaagtat gcacatttag ttcagtctgc atcgaatgga gcaattacga
    27661 atttactcta atctactcta aaataaatga gtgagtgcga ttttaaaatc aatctcactc
    27721 atctaattta gtttcaactt tttattccaa gtaaagaccc taacaaaaag aattcccatt
    27781 aaaaacaaat aaataaacca aatttcgatt gaatagaaat agttatcgcc taagaaagat
    27841 acagccaatt gatcaaaatc ttcctctcta gccttggttt cataaaacaa atttaaaaaa
    27901 aatctagcat tcaagataag attacacaaa gtagtgggat gaaggaattt ttagtcatca
    27961 tccctgttta caatgaaaaa gccacagttc tggatgtagc aattaaaacg atttccgcct
    28021 gtattgattt tgctgatatt ttatttgtaa atgatggttc ttccgatggg acagaggaag
    28081 ttttaaactc aattcaaaaa gaacatcctt acttgtttgt gcatcacaaa aaaaatggag
    28141 gatatggttc aagtcttatc tatggatttg aatttggggc aaatcgaaat tatccgtatt
    28201 taataacaat ggattgtgat gaacagcatc aaccaaaaga tttagctcga tttgcaaatt
    28261 ttaacagaaa gattgatgta gtgagtggaa gtcgttatgc gccaaagtcc agatcgattg
    28321 ggattgttcc tcctgaagat cgagtagaaa tcaatcgtcg cattacaaaa gcaattaata
    28381 aaaaatatgg ttggtatatt acagatgcct tttgtgggtt taaacgttat tctacaaaaa
    28441 aaattaaaga ttataaattt gaagagaatg gatatgcctt cccgatggag ttttgggctt
    28501 atgcgtataa aaacaattta agtttagaag aaattgctgt agataaaatc tatattactg
    28561 atgatcgaag ctttggtgaa gacctagata aaaaaagaaa gagatataag tattatctag
    28621 aagcatggag gatagcggaa agaaaattaa aagattacca ccgataacgg aacgacaata
    28681 tattaaatca caaagaaact ttcgagaaat aataacgatt tacaggttcg ttggaggatt
    28741 acttttcttt gtaaggtaaa aaggaaagtt ctttaataaa ttgataatct ttgtggtttt
    28801 tgttatataa ctgattgaga gaacaaaatc cttttctagt ttttctaaaa atgaaatcgc
    28861 cgattccttt tttctagatg aatcaattaa aatatctgta tcaagtagaa cgttattcat
    28921 acttaccaat tttttttctc agttctttaa cataggcgat actattttcc atttccttat
    28981 gatctttgaa tgcattgatt aaagggcttt ttcgaatccc tctttttttt gattttgaag
    29041 cattccctat taactacata cttagtcgct aaaaattgta taaaatcatc agcttccttt
    29101 tgcgccgatt taggtaatga ttgatatgtt ataacagttg aatttcccat agtgataaat
    29161 tgacaatctt catcgtaagt attcaacttt aatacccata tcgcttgcac caactggctt
    29221 attgcgaata tccactttcg tagtaaacca tacaaatttc gttttcaagg tctgaatgta
    29281 agaacttata ttcgctttat acctagcctg catagttgaa tttttaatta atgcttcatc
    29341 ttgctccagc caaagtatta taaaaacttt taaatctttt tctaaaattt tctgtgctat
    29401 acttttccaa aatttttcat gatttgttga attccgcacg ccaccagata tacaagctag
    29461 actacttttt accttctgac ctacttcaat cataaggtta gtgccttttt ctgccaatcg
    29521 gtattgattc tctattcgat gggctctaaa atcttttact tcaataaaat aaacaccgtt
    29581 tcgataaacg cccagaaaat ctacaccttt tgaattcggg agcgtatccg ataatttttt
    29641 ataatcagga gttttatcaa actgaattat ttcttcccag ccatcaaatg caaattctaa
    29701 atcctcttca ataaactttt gttcactcat ctttcattga cctctgaaat aattctcttt
    29761 ctttatcata atggttagca aactcttcta agattggatt gttttgaatt tcagtaagat
    29821 tttttccggt atgtactttt acaccttcct tttctgattc ctcaaggcaa aaaaatctga
    29881 tctcagctat ttcctccgag tacgatgatc tatattcatc cattaatgaa agttgatgcg
    29941 agagaagata atcatgtgta gccatgaata tttgaatacc agacattgcg agatgattta
    30001 atatcaaagt aagaactgaa attaacttag gattaaaata aatttctggt tcatcccaaa
    30061 ttagaattga attttcagta attgttccat tttcaattaa atacagaagt gtagccaatt
    30121 ttttcatccc ttcggctaac atagaagctt ctatttcacc cgtttctttc tttatataaa
    30181 atgtattgcc ctttttaatt aatgttcctt ctagttgatc tcttataacc gctaaaatct
    30241 ttttctgaaa ttcattttgt tctctcgtaa caggttcgct caatgctttc gctaagaaat
    30301 acaatgtctc atcatactta acatcgtatt tttcatagaa tggaatgaat tcagaataag
    30361 ctgtaatcat ttcttgtgtg ggaaaatagg ttacattaaa agtatgttca ataggtattg
    30421 atgtaatgtt ctgatcaaat ccggatacta gtttttgatc aaacgcataa tactgaataa
    30481 agaaatcttt atcctcatca aaaatcttat aattaattgt gaaatcttta tcttcaaagt
    30541 tacgaatgag tttcccgttg gactcaattt taaatatacc ttttattcta ttattgattc
    30601 tctcattaaa agacaattta tctttattgg caaggctact tgactttgct attgtataaa
    30661 gaatttttaa taaatgagtt tttcccgtac cattcttacc aataatcaca ttgattccgg
    30721 gcacaaactt caatgattca tgctcgaatt tggtaatgtt attgatggat aaatctgaaa
    30781 atttataatt caaattagtt tctcctttaa cctacttttt ttacttcatc caataggagg
    30841 ttatatggaa taacccctcg ataagccctt ttcattgttg ataattgaac tgaaacaagg
    30901 aacttaaagt tacttgttgt gcctacccac ttatcgtttt ttagaaagga attcaaatca
    30961 tttttgtatt cctcttcacg aaaaatagaa tgttcttttg tctctgtcat atagtcttct
    31021 aaaatgggaa gtgcggcatt ttcaattaac tcagacttgg cactttcatt ttttaaaatt
    31081 tgaattagag acttagtaat tatttccttt tcattggttg gcttattaaa tgccttataa
    31141 attatatccc gcaagatttc tattactatg aagtagaaaa aatgccttgt ttgttttctt
    31201 gactgtaatt cagcaactga accgaactta tggaagttag cttctttctg taatagaaag
    31261 cttgcataca aatcttcaat atttatataa tttttatctg ctttgtcagt gatttgttta
    31321 aagatacttc caccgggcgc aaacggagga ttctttccgt atgccaacgc tggtcgctta
    31381 agccaacctg acgcaaaaac ttttatgagt tcaaatgcat ttgcataatc atcatctcta
    31441 aaaaatggaa gaatagtagg attcaatttt tgtaaagctc gtctagaatc ccagccacct
    31501 ctttgaattt ctaagtaaat attgtattta tctgccattt ctgcatgaaa ccgattaaag
    31561 ctttcttcta atgaaataaa atcagtagct ttgaccgcgt tttggctatt agtaaaccgt
    31621 gtgattgatt ttagaagttc agcagaattt ttatcttctg taatgtctcc aattttcaca
    31681 atttttaaaa caacagtaga tgagttcagt tttttttccc aatcatgaaa gtgtggattc
    31741 tttgtattat tcgcaaattc atattctaaa gttgcccata tagtcttaga ggtttgacag
    31801 ccatttacaa tgtatggttc aataagatta gtctcttctc cttttttata gtcataatca
    31861 gcagcaacga tcgttacacc gttattatat aatccaaaac tttctggatt ttcaataata
    31921 gttcttctta tatcagagtt tactttgttt cgacttccta aatacttccg gacatttttt
    31981 tgatagatta aatctaaatc tttcttttcc ttcttgtaga cttttagaaa attatacaaa
    32041 tctattagct taatagatcc aaccaataaa tccgaacccg actcaactaa gttggctttg
    32101 atatcaaaaa tatcagttcg atttctttct tctaatattc tattgtagat tgtgcgaata
    32161 gagataattt caacatctat attaattgag tcaggttgct cttttctatg aatgtattgt
    32221 cttacaaaat cataatcatc ccatttctgt tcatctgata aggctaatac tatcgcgatt
    32281 ttatatgata atttttcatt tgccttggaa gctttagtta gaaaactttt taaattttgc
    32341 tgaaaaggta aatcttcttt tgttataaat ttctggttct ttatagtctg aattaatcgg
    32401 tcataatatg attcaaagga atctagattt tttagagcgt cacctaagtc tgaatagatt
    32461 aaataccaaa caaactcttt attagaattt tcttcttcat cagacccttc atgataatag
    32521 gctaaatcaa ctcccagagt gtaatggtta ttaaactcaa aaaaatctgc cgaatcctca
    32581 tctatctcaa accactgact aaatagcttt ctgccaaatt cactacctgt ccgctcaggg
    32641 ttcttacctt tctgcaaaac tggagccaac cactcatttt taaatgtttc ataattattt
    32701 actatttcta acattaacta cttcctctga atgagatagc caactgattc aaatacgtca
    32761 cctaataaaa atttctttct aagattttcc agatcattca attaactaca actttgctca
    32821 catatctttt gttctaataa gaaaaggcga atcactctta caggaatacc tataatctct
    32881 aataaaaacc ctagcttaaa actcgtcaac ttggattaag catagctctt tatgtctctg
    32941 cttctttgcg tgagactctt attaacaata cactaaagct cctctaaagt taggactaaa
    33001 cttcccttaa agatagggta atgagatata tttaggggaa gtttaaccaa tgtttacctc
    33061 taagttcaat agatgtttgg tataagcaaa accaaaatct tccgtgctta ttctaccctt
    33121 cgctccaagt attcccgacc agcgccttcg catgc
//
LOCUS       DAOQOS010000056        33050 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_586_length_33050_cov_6.748841, whole genome shotgun sequence.
ACCESSION   DAOQOS010000056 DAOQOS010000000
VERSION     DAOQOS010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 33050)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33050)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33050
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_586_length_33050_cov_6.748841"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..516
                     /locus_tag="PLF66_13435"
     CDS             <1..516
                     /locus_tag="PLF66_13435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015676638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65096.1"
                     /translation="CHSAGCYATEYWLSNLGGTASSKGFKISGVTALAAASGGSELAN
                     SLNAVSFGYLGNAMDVALIVTNARSSFNHNNTAGVTVAHVPGYKGMIGASAILPGEDD
                     YAVAYHSSCGYSTVGGLSKCQSSITQYEGIWPFGGNVTYAQYLGHTRAPSVPVDGLYE
                     NHSEITVEGWR"
     gene            complement(672..851)
                     /locus_tag="PLF66_13440"
     CDS             complement(672..851)
                     /locus_tag="PLF66_13440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65097.1"
                     /translation="MIAEKKFLCVIWVGSEEAFILLYLTFDSVFPQFKKPKFKTKASF
                     AKFSCILFKFLNAID"
     gene            complement(1141..2745)
                     /locus_tag="PLF66_13445"
     CDS             complement(1141..2745)
                     /locus_tag="PLF66_13445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016561193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HMZ65098.1"
                     /translation="MSEIIESKIDVNSTEFKENYKDLELKTEQIRKLINRVETGGGEK
                     AIAKHKERGKLTARERIKYLIDKETTFLEVSALAAESVYPDIVPSAGIVTGIGRVSGI
                     DCMIVANDATVKGGTYYPLTVKKHIRAQEIALENRLTCIYLVDSGGAFLPKQDEVFPD
                     KEHFGKIFFNQAQMSARGIAQIAVVMGSCTAGGAYIPAMCDESIIVKGNGTIFLGGPP
                     LVKAATGEVVTPEELGGADVHCKTSGVTDHYAENDEHALEITRTIVSNLNLKPKRETL
                     SFSEPLYPANELYGIIQRDTRKSYDVKEVIARLVDGSKFHEFKKLYATTIVTGFAHIH
                     GYPIGIIANNGVLFSESALKATHFIELCNARKIPLLFLQNITGFMVGKKYENSGIAKD
                     GAKMVNAVSTSVVPKFTVIIGGSYGAGNYGMCGRAFDPRFLWMWPNAKISVMGGEQAA
                     NVLLTVKLDQLAKEGKTMSPEEQAEFKRPILEDYENKSSCVYSSARLWDDGILDPKET
                     RHALGIALSVAANQDIPEPKYGIYRM"
     gene            complement(2748..3785)
                     /locus_tag="PLF66_13450"
     CDS             complement(2748..3785)
                     /locus_tag="PLF66_13450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010572769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase"
                     /protein_id="HMZ65099.1"
                     /translation="MKNKVQRVLINESITPDGLRIITRFKSFSEIRNGIFTTLQRIKF
                     QYPNAFIYYKNPDIFFTKAFLERNPECKLYENEEFDLEITPNDYLSWKLLPLIAKQIE
                     EDLNLYESAWKWQKKLKVKIEDYSVFGKKKHLYIHPSAIIYPNVVFDVSSGPIIIDKN
                     VKISPFSFLEGPLYIAEDAQIDDARIGGASIIGQACRIGGEVENTIFENFSNKHHEGF
                     VGHSHIGSWVNIGALATTSDLKNNYGIVKIKIGEKQISTDTIKFGSIIGDFSKIAIGV
                     MLNTGTVVDIGSNVIKSRVGGYIHPFSWSESQERYRLDTFIQDTKKIMARRNQTLRSE
                     EEALLKKLYEG"
     gene            complement(3795..5627)
                     /gene="glmS"
                     /locus_tag="PLF66_13455"
     CDS             complement(3795..5627)
                     /gene="glmS"
                     /locus_tag="PLF66_13455"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010572770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HMZ65100.1"
                     /translation="MCGIVGYAGNKSVESVLMVGLACLEYRGYDSAGIAVIDNDELEI
                     RKEKGKIKALEALLKEKPVRGNVGVGHTRWATHGEPSQINAHPHTNEKQTIAVVHNGI
                     IENYMQLKTKLQSLGYIFQSSTDTEVISHLLSMELSQKKSIKEAFLSLFKMIQGKWAI
                     AVVFEKEPEKVYFAQDGAPLLIGKGKKEYYLASDLSALVRNSSEVCYLKPKQWGYFTK
                     EDLELFDFDGNSVAPEMKKQEAKWEDVDKAGFPHYMLKEIYEQAGIFRKVIERRINEN
                     GDLDFSEMELSKEILSKVNRIVIQAAGTSLHSGMIGKHYLEQFTKIQTDTEMSSEFRY
                     RNPVVEGDTLIIAISQSGETADTLASIHEAKAKFLKVLSMVNNVNSTIARESDAFIDT
                     CAGMEIGVASTKAFTAQVLHLLLFAIYLSSIKWMMSDELRKELVEEIKQLPTKIEFIL
                     NKAPELEKWASDFINTKDFVFLGRTYNHPIALEGALKLKEISYIHASGYAGGEFKHGP
                     IALITNEVPVVCIANKSEIYPKMISNIQEIKARNGKIISIVTEGDVEAKSLSDYCFEV
                     PPCHEFLSPILNVIPLQLLSYYVAIARGCSPDQPRNLAKSVTVE"
     gene            complement(5632..7008)
                     /gene="glmM"
                     /locus_tag="PLF66_13460"
     CDS             complement(5632..7008)
                     /gene="glmM"
                     /locus_tag="PLF66_13460"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004475442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HMZ65101.1"
                     /translation="MNYREISINNSNSLMISVSGIRGIIPTGLNFENIIQFTLAYANV
                     LKPKTIIIGRDSRPSGEYIENIVSGVLLGIGINVKTIGIVPTPTLKSIVKETKSDGGI
                     IITASHNPLEWNAFKFVGKNGFFFDAKQIQELKDFIQKGDLPLPKFSPKSIKSNSSES
                     LIDLHFESVLKQIDLAKIKKRKFKVFVDAVNGGGSLVIPEFLKRLGCKVYETYCTPDG
                     TFPRPPEPTPKALIKTAKLMKKTDAEIGFALDPDADRLIVLTPKRGVISEEYTLPLSV
                     QSVLGRKGKDMVVNLSTSFLTQEAIARYGKKVHLAKVGEANVVSEMIQKKAFFGGEGN
                     GGVIDPNISSFGRDSLAGIGHILNFLASNKSSVDTEMDKIPKLYMQKESISVQGRNLK
                     DILEKLKDNFKGSKINEKDGIRFDFESSWVHVRASNTEPIIRIIAEAKNENDLQALLT
                     KTKQRIES"
     gene            complement(7042..7114)
                     /locus_tag="PLF66_13465"
     tRNA            complement(7042..7114)
                     /locus_tag="PLF66_13465"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7079..7081),aa:Val,seq:tac)
     gene            complement(7236..7502)
                     /gene="rpsT"
                     /locus_tag="PLF66_13470"
     CDS             complement(7236..7502)
                     /gene="rpsT"
                     /locus_tag="PLF66_13470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004785420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HMZ65102.1"
                     /translation="MANLKSSKKDIRRAEKRRDRNSKRKSAIRTFAKSVLKSVKAGKK
                     EDAMNFYKEYSSLIDKAAKNKIIHKKTADRSKSRLSKKIAGIKN"
     gene            7648..7926
                     /locus_tag="PLF66_13475"
     CDS             7648..7926
                     /locus_tag="PLF66_13475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65103.1"
                     /translation="MYPILISLDEPILHFLVSEKIDLSILLKIGLLKEESSDFQYFHS
                     ENKKNIRLNLERSYFVLLNSFCVKNHSYMHIVAGELILEIILQILSYE"
     gene            7919..8899
                     /locus_tag="PLF66_13480"
     CDS             7919..8899
                     /locus_tag="PLF66_13480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65104.1"
                     /translation="MNKDNEYIKVESISEIQNNLKVDLLNKKFQDKDGNRFVVRFGNE
                     SRKLEILRIITKSELESGHIGELETKNEPSHDHKIDLEKEREVEEQKQKENLRQADRQ
                     EINSKFNEDSGDGFGDIDLNIEAVDTVHKKVHDHSLINPRSAADERRYIFDLLKAIDK
                     NKDRVVSILTNVQNSRIYELTGDPSENKNIISNFTREFEGDIFRILENLQNKFKEITS
                     FPKTTHHYSMNYTVLQKDFLAKLETDKAKFDYILRWDFQEPTLILIKKFAKMVTDLLN
                     VLNKKEEDQIKTLSFQQQTLFRDAKIAGVYCLNDLDFLKRSVESWKESAV"
     gene            8909..9706
                     /locus_tag="PLF66_13485"
     CDS             8909..9706
                     /locus_tag="PLF66_13485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65105.1"
                     /translation="MPELSNKEIEALLLKIRNEYKTYAQENPNIFKLAPFEERYTQIL
                     RSRGSLELFFKDELLFLEQLKAKHKELKARREASQAPTIGRIMEENETRILKYNKIDF
                     HPMAKNELKYFYGAISNFVETDLQLLYQIFRGTAEINSIHDSIIQLERIGLNRKGQLP
                     FRISEHMKILTSVSVNQGKIEQDSQSIMKDGCLALKHISMYLEDAEKNKKINMKLIFK
                     FNEKDYPELFKKYNGISFDTGAEMIIVNCANIITDFRMDSIVGLKPR"
     gene            9703..10392
                     /locus_tag="PLF66_13490"
     CDS             9703..10392
                     /locus_tag="PLF66_13490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004751232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HMZ65106.1"
                     /translation="MKLSIVIPCYNEKNTIQNILEVVRKVPIKDREIILVDDCSKDGT
                     REILQNQLKGLVDKLIFHEVNQGKGAALRTGFQAATGDIVIVQDADLEYDPFEIPTVI
                     EPIVQGKADVVFGSRFLGGGPHRVVYYWHRLGNMVLTTLSNMFTNINLTDMETCYKAF
                     KRDIIQSIKIEENRFGFEPEITAKIAKIKEIRIYEVGISYYGRTYAEGKKIGWKDGVR
                     AIWCILKYNLF"
     gene            complement(10618..11049)
                     /locus_tag="PLF66_13495"
     CDS             complement(10618..11049)
                     /locus_tag="PLF66_13495"
                     /inference="COORDINATES: protein motif:HMM:NF020950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease inhibitor I42 family protein"
                     /protein_id="HMZ65107.1"
                     /translation="MKNYIIITLLVASFLATNALSANSKKRIVKKVNHITTIEDESDD
                     ITIKMKKGKLEGIKLPVKMATGYSWILVKHSDNLKQPFKPRIIQPKKKEEDKEVSIKE
                     FQIFYIRALESGSGMVEFEHRQPFDKESPPDKEIKIKIEVE"
     gene            complement(11063..12286)
                     /locus_tag="PLF66_13500"
     CDS             complement(11063..12286)
                     /locus_tag="PLF66_13500"
                     /inference="COORDINATES: protein motif:HMM:NF012340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HMZ65108.1"
                     /translation="MKKNKLQPRKILLAISLFSFLFSTLYIHSQTSESMKDILQKQKD
                     ANKQNLDDLKAKLSKLEQEIKDKNYSFKVGITEAMKYEISQIASTKPPRSIDYNKVNQ
                     EAQKRLKMEAKRKKQKKKVKFTSDDSEETEEFDYSEEDPLPDEFADMQDYSEDDPYQQ
                     TEDKGYIEPPPPPKKNPSKCTVDLKAWDWRAEGKVTPVKQQGSCGSCWAFTTAAAFES
                     SYLIQNNKSIDMSEQQIVNCSRAGSCASGGWPGDAFDYLLMKGPTSSGSRSGIDESAE
                     PYKGKDMQCKPYKTTSYQTVAWGYVANGNRTPTVKEIKQALCKYGPLASAVYVTNHFQ
                     AYTGGVFNEKVKTSSASDVNHAITIVGWDDSLNAYLIKNSWGDKWGIKGYMWIDYKSA
                     NIGYGTMWVVARNEK"
     gene            complement(12401..13288)
                     /locus_tag="PLF66_13505"
     CDS             complement(12401..13288)
                     /locus_tag="PLF66_13505"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HMZ65109.1"
                     /translation="MNFILSTIQNELIASREEEEKDLLEKGIFLAEYLFHFVEKKDTT
                     ILIDKDRKIIHYREGNLPFNLKTGEFLPNETITSKALLTNTFIKTKKSKEESAFQIAY
                     VGSAIPIQNKNGNAIGAIGWISSVESWDIISETSSVTSKSIQFILDSFHSIHTSSQKT
                     NNSAKEAYEQILATNKKITNINEVIHMIKKIADQTNMLSLNASIEAARAGDAGKGFEI
                     VASEVGKLATHTKNSLASMTTGLMEIIQSTQLLSNKIENIKNDSNRQDETIETMKVRV
                     ASVIRDLKSIEQLAKKITN"
     gene            13891..14832
                     /locus_tag="PLF66_13510"
     CDS             13891..14832
                     /locus_tag="PLF66_13510"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004785482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HMZ65110.1"
                     /translation="MAKKKYANSFTLFGYSIFHPLNLILLTNCIIFLMQLGENMTMTY
                     LFALTPNLFFSGSYWQIFTYGFLHSTFFDLIPFHILMNMYGFYMLGRYLDPIIGKAKL
                     AGLYFASQLGGGIFIIGYALLMKYLVMPDNLELILKTPTLGASGAVFGLLAVFGLLYP
                     EEELILLIFPARAKNAVFLLLIIGFMFEYFFNAPISNLGHLGGALVGFLFYYSFLRKS
                     NNRIVFRITFNPDELEPRKEIANDPFRNQVEQNLRIRHQIKTISSLKDKEEYLQPYQI
                     PNANICPPSTYNTEDEFCLRCEWFGNCMLRKSKMENE"
     gene            14834..15937
                     /locus_tag="PLF66_13515"
     CDS             14834..15937
                     /locus_tag="PLF66_13515"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HMZ65111.1"
                     /translation="MKKTFQKKILLVLILLGLIAFIPLISDSIDILTDDDKDGISDAL
                     EKYAGLDPKKDECEPNGCSGFYGDEYLVMILDQSGSMAEKIGSDTRMEAAKRVSIKLI
                     DKFPATAAGLGVYSYGTSTTITGEPAEDTSGCKSFNELQSPFKRLDRKELKEHISKMQ
                     PDQSTPLAFSLLKFRESILDKKGKFNLILITDGEESCNGNPVEEAKKLVMLNSKTLAI
                     KLSVIGFGVDDKTAEELKKIAIASEGTYQSVASEEALEDIFQKPIKDIIASFQGILCL
                     HKQVDKLLLCEQSRDNKLRAAFNRLNSPLSSPFSDEDKKTIIKYYPETEKYIQARIQA
                     YTKIKKEGTESYVKKIDELSKMIVSPADRIKKK"
     gene            15998..19033
                     /locus_tag="PLF66_13520"
     CDS             15998..19033
                     /locus_tag="PLF66_13520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65112.1"
                     /translation="MNPFLTVLKNLWKRKLIRFLFILAVIYKLVFNVFTGQVLIEKIF
                     HSISTGTVSLNVKRFSLFFGIIIENIKLSSGFDFDNKPVLTVDRLAVTYNLPLLFTGR
                     LKLTEISLTKPELHMWQKNGKWNVETLFVSSEEKKEEEKKEESTESSNELSLPIPVSG
                     YLNFFIHDLKIEIEAEKGEKYFYAFLDNFSFYFLLNTKRFSKLPYSTKAVSIIDELKI
                     ELNPKEEISLSMKDNIAALQTKMRLSLLLNNDNTGDVPILTSKLDIGSDKIPIKMKTG
                     DVQPFNFKIGYDLNFIPEKDIVELKDLIFSFAGDKLFEGKGTIKDALKEDPEVQFEIV
                     ESNINLDNVERITDTVPIIPPMNLSGFFSLRPFTVNGKLSDLHVHSGINGKKIVVELF
                     RKIHDMPVIDFQFDADLNLLTKEESSDINILPQLKRSIIHNLEGQYNGIVLKIKGEVS
                     PKEKVDLFLDLDRVIIEKFVPDVLGRAHLYITAKGDKLSFMNLDLKAVLSGLRYKLGR
                     GLSGVNQVELNLKTAIDFKKDFQLETLKVEPLTFAVKNENKEVAARIDSAMNLDMKNG
                     TKAEIDKLKLEANITHLIPTVPVSLRGTIAGLRQSLGNILTLNGKASYKEEAKNKIIN
                     LGLGASFPALELTDMNITGLINIAKDEKETLTIDNITLSAFQNKFKGLFKGNLFKPKT
                     ANPPFGEYAGEINGNLTLESDVFRYVLKGMSFKGDLDIDINVKDHLISGALRSNDSYI
                     VYKTSECPGKGCQVYELNGLKMNIPFLHDINDKTTEEMLDGNKTNFVKTYGLDAPPNL
                     SIQSIVGTHPSLENAKLDFVKSISGSPGFVARVDYIENFLTIDNMKAYSMNGYIYGKD
                     ILFNIGNGDPAQIEYSAVIQVRDIDLSELLPKDMRKKIDDGKIKLDLNISGQNLNDPI
                     ENLELFFSTFYIGEDFGKSAIRIVSPENVITDQIIKSYRVDKIELELTKGLVYATIKF
                     QKSVFNTLVFNVENDMISQERIPLASFLKRTEKELSNYK"
     gene            19041..19646
                     /locus_tag="PLF66_13525"
     CDS             19041..19646
                     /locus_tag="PLF66_13525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1318 domain-containing protein"
                     /protein_id="HMZ65113.1"
                     /translation="MYTRLYIFPILILMNCTFLSLRPPAITFTQSQTAAERQMIGEGK
                     DLEKDGWIISSIRTSASGSDVWEKEILDKEIPEGELDEVTYTALKRLAYLSKDVRDFK
                     KKDFIGEALDGQIKINPLLNESRFKKQFPESKKQIDELIQMVNESRRIVYESKLQKLN
                     SMNLKEQELIKKKDALQLSYYQLVEDGEYYEAKRGKWGRKE"
     gene            19649..27727
                     /locus_tag="PLF66_13530"
     CDS             19649..27727
                     /locus_tag="PLF66_13530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65114.1"
                     /translation="MIFSFFQKKYNGWNQIRLCFLFIFFANCSLLTYRVNEVKPNEFQ
                     YENISKAHFTPNNEKPYPLTVRRGNNIYNSTTKDARFLFFASDANGNFDIYMRDLQSS
                     AVLLVTSHPAPQYKPAISPDGKRLAFVSERYDSEGDIVLIDINPEKMIDSYLSGSYEV
                     ENFKQDVITNPNHKEISKIERVMDSDPDWSPDGRYIAFATKRFGGNNPDIGIIDTKNN
                     NSITRITTKGGATPRFAPDGKTIYYISFQDELMGEIYSIDINTKEEKRITKDKYMDFS
                     PTVSSDGKYLYYTSIRVDTNCNKKLDERDNSFIIRKDLTTGESIQLTAGNLSLFDTRY
                     SNLNGGSILFSASLENALNIYFIPKDGEIPKQETITKQYELARLYKSRSMDYFKLAFN
                     SMYLFFGQDPLFPLYRSRADKQIAIDYLEDGEDTKGENLLKEMLKTKGDKNQGLSYAL
                     SIVQQGIIKNKNPIPELTSYYEEMKNEKGVHPQMLPSLLMTIADYYKEINDISMAEKT
                     YQTIRLEYPNFYRIMEVKRKEGYFEYQMNPNKIPDSFIEIINNPNSNREDKKFVMDDI
                     IKMLGGQDDLEKKSRLIDVYLKEQKLDQVCPELAGLLYYTRAVILNEQDKFEESIKLV
                     NSYLTNINKGSYVYLKSKILQSDNYRDIKDEAKSNQELVDFVKNYDYFSGVEITEEEM
                     ERSFYYFETRAREYERKGELKESIQNFVYNNLLLSLAIEKKLPIQKTYQDFSVFYEKM
                     MVDTSFNLSRYVKDKDLKNIINKFNLLGRRQLDVAGRTTRSLAYFFRWKYFRIFGDFR
                     DLQLLFPMEEDAFQQSEEYFKTRLKVARENLEYATIYGYAYYLVSKAVQSETYFINED
                     ALTRARKAKILNQLKQAEYDLQWIIYANPNYTDAYLLLGWLYQYIDVRKKGLVYPENE
                     TDSEVFESLYAKFFPYKYLEENISLYSQILQFLGPNYENKKALSDIHLNLANNYFLLS
                     NYPKAFTEYQEVENLSKFILEKNRFENYRQKAVYYFNLARIHVYNGKIKESIPYLKNA
                     LELYYKNEYFPLLAKIGTKNIPELNSYLDDVKKKIALLNALLGLVQMELRMYSDAITS
                     FSTAVSMNGKSDYINDISLYNSLAFCYQELGDYKKSETSLKLADKEYSRKKRRLAEIF
                     SFSIGDKFWSIALPDSARVIGEGRFPGEIPLDFENLITRGVRIANYIEKNEFDEVSRQ
                     IEDRSNFIKERKLDSTLIGEKILNNRLNELGFNEFSRGNYFEATKIYTEEYEDLKKKG
                     KLKEAFQSYIKSDIALYSHIEQNAEKKENLLKELNTNIKFLNKFKTEEILSCLKNYKF
                     EENTTDEMKEEICRNQFYTDYYNYDPYLGNNYFYAAEIYLTSKEYEKAFTYYGMTLPL
                     FKNPSGLQDDEIGLEKDLFTSKQRTRLRFLTAIVYLRIGETEKFEKILRESYYLANEF
                     QLEKELISYYILEAEYNFKMGRYKKSIEFCERAENSLKSSPGLWYEIDEISINNIYSL
                     KSANLVKLKNYTDLASNREKLFSAIFFRQLMINELRFQDKEIFKTINNLQLYVWEDKE
                     LIYKIEAINLAKGDPKEVVTAKAKNLENILYEMKNLEKILPNGMDALSWYSKPKKFIP
                     KLKSDELLISYYTTGLDGTQVILNSNNNIEFNDFKLNGKRDTETFSRELEGILQHYPQ
                     VKKLVIIPSPLMYDINFRLLPYKGKPLNETYEVRHLFRLSQLEREADVEFSRLKRITS
                     VNPNIKVDTEKGIDLNPINLVLPKVEPKKDMKELNLRIIQSNELKSYLTDTDILEGPT
                     DFSNRKQYIGEKRPGMISMKDVVEDQWTIPFIILTNYTKSMDNYIKTGFLYDILQFSG
                     VQSIILLEPTAANAKIRDNLISNIKNANKIIKEEKLHLVGEYINEYPENQRIFESEFK
                     KYTSLAVKEERKRNFLKSMKYLLQANSVLPENNAEYLIRSELNLAKLKTKTFPSVSNY
                     LVHYENLLNRFEINSREEEAILYELLISCYEAPIAVNCDKYFSRYNALSNATDEKKYV
                     VNYYKNLREGNLNIIDPDYDKFISIKTEEDPYLKNMRLASLFARGFIWDKARQHAEIA
                     KKLAESKQEVNYADNAISDIEYEIFFIKGIDPDTIREDKIYFYATNRIWDAYRKKARE
                     LAENQSDASRRTYQSRLLDAYDSIENKADFEPTTLGPLYLKDGRPALYLLKESERDFL
                     FHLLLKSIITQTGEELNNQFDILIETELRLRNRNRALWMQIQWAGALYHRGDYESAKR
                     YFNEFDKNFRDYYPEKSLVKTFYTLKYKLSRVFPEIEFATSERDFIKEEFKEWFGYYE
                     ICEQKQPIDFLPILNQLVASKKKEKLDLFNARELDDFISFLQLESIEKKDYAVLADLG
                     FAKDKYKSINDKVLGRQVLFSDLPEFKSISRALVAKLPKDQIFSAVIDLGIKSYVVRM
                     KEGTITNELGFNDNRDIKYSILDYYYTIKDGGVSIVKQESIEARYKEILKFSKNKIDY
                     IYLPSYHFKAVIEPDEQDYFYYVLSPELLLERPIYDTTQDFVPGFKVEAKNISTYSPK
                     WWRMIQELEKYELRNVSGVGKGNRIQISQEDLLLENGRKLQFANMGLNEIKRIEKRKG
                     VWVMTGNLLSESSVQNDDFPHSLRYIDSIHQGPGVVFASLHNQTATAFFLKNFFKNLN
                     YRYPFIERYYDAFTTTQEEFPFDKYWNGFRPYTNVFIK"
     gene            27756..28217
                     /gene="tsaA"
                     /locus_tag="PLF66_13535"
     CDS             27756..28217
                     /gene="tsaA"
                     /locus_tag="PLF66_13535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008590699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HMZ65115.1"
                     /translation="MEFTIRPIGLIRSERLESIDDHWNQVRSFIELTDEYSSESIRGL
                     EDFSHIEIVYYFHKVKASQIVIKAEHPRENPNYPLVGIFAQRKKSRPNLLGLTISEID
                     KIEDKKIFLKSCDAIDGTPVIDIKPVFKEFLPKTEIKQPVWVSDLMKDYWE"
     gene            complement(28377..29192)
                     /locus_tag="PLF66_13540"
     CDS             complement(28377..29192)
                     /locus_tag="PLF66_13540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HMZ65116.1"
                     /translation="MQSKKQTAICLNSAFFGFYAHAGFVKGISELKFKPSFVSGCSAG
                     AIVGALYASGVNIDEMISIIKEFKKKDFWEGNIFSHLIKPFRHGFKNYSGQLTGVKLK
                     NFLHPFLGNKKIEDFPIRFGAATVNLTKRKREFITKGDAIDAILASSAFPLLFEVQKI
                     NGMDYTDGGLIDHEPTKELILDKSIHTIITHNVHKEQEESKYILGRAVSQGLSVIEEE
                     TRDLKDLLAKKYKTKIHRITTITPAVDPDKLHIGIGNIELGRQSVHKNAKLFK"
     gene            complement(29401..31026)
                     /locus_tag="PLF66_13545"
     CDS             complement(29401..31026)
                     /locus_tag="PLF66_13545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016754788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HMZ65117.1"
                     /translation="MKIKIILFLSLLVFSSSNLFAKRYGLILGSNYKGNKAGISELNL
                     CEADATYINDQIKKVGNFDEIKVLLGSQITKANIEREIKAIGAKATTSDVVFLFFAGH
                     GFFQRDASAKNGMRNYLVCYDRPHLSDDELNDYLKTIKTQKTVFVFDCCFSGGIAKKG
                     KATRGDKDVPIPDGSQGTVRQDPEDFYFQNKAIISSADDNQTAIEVGGDINHGIFTYQ
                     FGRALESGDLNGDKVVTALEAFFKSRDAVIKMAEDNDHKQVPQVSGDASGIFLAGNVT
                     PVIPPPPVDPKPEPKPEPKPEPASDPVKPDPVQPPVTPAEPPVINVTTNGDLLIKTTI
                     IRDRAYGLSTLPPEEMMKQKKVREGDRRVKVLIDDKEVNFKLIPIKSDFWGSVVRGGR
                     LIQGEIYHIRVSKVATGVHKITIKADEYPEVVTTFAVLENKDNVLELTNAMSGFGVIQ
                     GNVFYKTLDNPVIKQPIYMPTVKSVNNVQKVLTDSEGNFWFTNLIPGDYEVKASFAED
                     LKLNNSIIKVKEGEVTRVQVILNVKLPLTKTKY"
     gene            complement(31189..32190)
                     /gene="ilvC"
                     /locus_tag="PLF66_13550"
     CDS             complement(31189..32190)
                     /gene="ilvC"
                     /locus_tag="PLF66_13550"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020769171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HMZ65118.1"
                     /translation="MAKLYYDADCDLSVLQGKTIAVIGYGSQGHAQAQNMKDSGLKVI
                     IGLKDGSKSIKEAKEAGFEVYSIAEAAKKADIIQILAPDTVQAATYEKDIQPNLQPGN
                     AIVFSHGFNIHYDLINPPKDIDVYMVAPKGPGHLVRRVYTEGGGVPCLIAIHQDATGT
                     AHKRALAHAAGVGGGRAGILETSFREETETDLFGEQVVLCGGLAALIQAGFETLTEAG
                     YDPEIAYFECLHEVKLITDLIYEGGLARMRFSISDTAEYGDLTRGPRIIDAGTKARMK
                     EVLNEIQKDKGAKFAKEWVAETKAGYPNFQKLRDKGAAHPIEAVGKKLRSMMKWLAK"
     gene            32728..>33050
                     /locus_tag="PLF66_13555"
     CDS             32728..>33050
                     /locus_tag="PLF66_13555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65119.1"
                     /translation="MTKKDEPIFYCETCGRAGDYLDFVDLSDEEIDPFSIYTVCSECL
                     NGMRKNELVGIILLVLYLISGIFVGLEVKNQLNLLFVIYSLNFIRRILYFRFLKNKIR
                     RDNKS"
BASE COUNT        10850 a         5322 c         5803 g        11075 t
ORIGIN      
        1 tgtcacagtg ctggttgcta tgcaactgaa tattggttat ctaacctagg tggaacagct
       61 tctagtaaag gtttcaaaat ctctggtgtt acagccttag ctgctgcatc tggcggttct
      121 gaattagcaa attctttaaa tgcagttagc tttgggtatt taggaaatgc tatggatgtt
      181 gcgcttatcg ttacaaatgc aagatcttct tttaatcaca acaacacagc tggtgttaca
      241 gttgctcatg ttcctggtta taaaggaatg attggggctt ccgcaattct tccaggagaa
      301 gatgattatg ctgtagcgta tcactcttct tgtggttata gcactgttgg tggtctttca
      361 aaatgccaaa gttctattac tcaatatgaa ggaatttggc cgtttggtgg gaatgtaaca
      421 tatgctcaat accttggtca cacaagagct ccttctgttc cagttgatgg tttatatgag
      481 aatcatagtg aaataactgt cgaaggttgg agataaataa aagtaaaaag gaaaaagatt
      541 accacaaagt gtaaaaactt tgtggttttt tcatcccgcc tatcgtatct ttttcatttc
      601 gttcaaaaaa aataatgaat actaatctag tttgaatctg tctatttgtg tgatatttaa
      661 atcattgcaa gtcagtcgat tgcattcaaa aacttaaata atatacaact aaattttgca
      721 aaacttgctt tagtcttaaa cttcggcttt ttgaattgtg ggaatacaga atcaaaagtg
      781 aggtaaagta ggatgaatgc ttcctcagaa ccaacccaaa tcacacatag aaatttcttt
      841 tctgcaatca tagtagattc ctcgttcgat atttttttgt gaatatgaca gtgattgtat
      901 aaagatccat ttagatcggt ttatcgaatg ggtatatgta aataagtgtg agtccatttc
      961 gttttctgaa cagaatgaag gcaatccaag aaaggtcatt tcgataaact caatgtttct
     1021 ctctatgggt cgaaacataa tgctcaatga ccgatttttt ttgtatcctg aatcaaattg
     1081 caataacaaa aaacttatca gtgcctatcg gtggcaatct tttaatgtta tggtaaaaac
     1141 ttacatccta taaattccat acttaggttc tggtatatcc tgattagccg caacggagag
     1201 tgcaatgcct agtgcgtgac gtgtttcttt tgggtccaaa attccatcat cccataagcg
     1261 agcggagcta tagacacaag aagatttatt ttcatagtct tctaggattg gtcttttaaa
     1321 ctcggcttgc tcttccggtg acatggtttt tccttctttc gctaattggt ctaatttaac
     1381 ggttaatagc acattagccg cttgctcccc gcccattaca gaaatttttg cattcggcca
     1441 catccataaa aatctaggat caaaggctcg cccacacatt ccataatttc ctgctccata
     1501 agagccgcct atgataacag taaatttagg aactactgaa gtagaaactg catttaccat
     1561 ttttgctcca tcttttgcaa taccggagtt ttcgtatttt ttaccaacca tgaagccggt
     1621 gatgttttgt agaaataaaa gtggaatttt acgagcgtta caaagttcta taaaatgtgt
     1681 tgccttgagt gcactttcgg agaataaaac accgttattc gcaataattc ctatcggata
     1741 tccatgtata tgtgcaaatc cagtcacgat agtagtggca tataattttt taaattcatg
     1801 aaattttgat ccatcaacga gtctcgcaat tacttctttc acatcatagc ttttacgagt
     1861 atctctttgg atgattccat agagttcatt agctgggtaa agtggctcac taaaagaaag
     1921 agtttctctt ttaggtttta gatttaaatt agaaacaata gtgcgtgtga tttcaagtgc
     1981 atgctcatca ttttctgcgt aatgatctgt aacccctgat gtcttgcaat gcacatctgc
     2041 tcctcctaat tcctcaggag ttactacttc gcctgtcgct gctttaacaa gtggaggtcc
     2101 tcctaaaaaa atcgttccat ttcctttaac gattatagat tcatcacaca tagctggaat
     2161 ataggctcca ccggctgtgc agcttcccat cacaactgca atttgagcaa taccacgtgc
     2221 tgacatttga gcttggttga aaaatatttt accgaaatgt tctttgtcag gaaaaacttc
     2281 atcttgtttt ggaagaaacg ctccccccga gtcaacgagg taaatgcatg ttagtctgtt
     2341 ctctaaagcg atttcttgtg cacgaatatg tttctttact gtgagagggt agtaggttcc
     2401 acccttcaca gttgcatcat ttgcaacaat catacaatcg attccagaaa ctctcccaat
     2461 tcctgtaacg attccagcag acggaactat atcagggtag actgattcag cggctaacgc
     2521 tgacacttct aagaaggttg tttctttatc aattaagtat tttattcttt ctcttgcggt
     2581 gagttttcct ctttctttgt gctttgcaat tgccttttcg ccaccaccag tttctactcg
     2641 attgattaat tttctgatct gttctgtttt aagttctagg tctttataat tctctttaaa
     2701 ctctgttgaa tttacatcaa ttttggattc aatgatttca gacattttta gccttcataa
     2761 agttttttta atagagcttc ttcttctgat cttaaagtct gattcctacg tgccataatt
     2821 ttttttgtat cctgaataaa tgtgtccagt ctatatcttt cctgtgattc agaccaagag
     2881 aaaggatgta tataaccacc tacacgagat tttattacat ttgaacctat gtcaactacc
     2941 gttccagtat ttagcataac tccaatcgca atctttgaaa aatctccgat aatagaacca
     3001 aatttgattg tatcggtgct gatctgtttt tctccaattt tgattttaac aattccataa
     3061 ttatttttta agtcactcgt tgttgcaagt gcgccaatgt tcacccaact tccaatatga
     3121 gaatgcccaa cgaaaccttc atgatgcttg tttgagaagt tttcaaaaat tgtattttct
     3181 acttctcctc caattctaca cgcttgaccg attatacttg caccaccaat tcgagcatcg
     3241 tcgatttgcg catcttctgc tatataaaga ggtccttcta aaaaggagaa tggtgatatt
     3301 ttgacatttt tatctataat aatgggacca gagctgacat caaaaacaac atttggataa
     3361 ataattgcgg aaggatgaat gtataagtgt ttcttctttc caaaaactga ataatcttca
     3421 attttaactt ttaatttctt ttgccacttc cacgcgctct catataaatt tagatcctct
     3481 tcaatttgct ttgcaatcaa gggaagtaat ttccaagata gatagtcgtt aggcgtaatc
     3541 tctagatcaa attcttcatt ctcataaagt ttgcattcag gatttctttc taagaatgct
     3601 tttgtgaaga atatatcagg attcttataa taaatgaatg cgttgggata ttgaaatttt
     3661 attctctgta gtgttgtaaa aattccattt ctaatttcag aaaatgattt aaagcgggta
     3721 ataatcctta atccatctgg agtaatggac tcattgatta aaaccctctg aactttattt
     3781 ttcatttata aaagttattc aacagttaca gactttgcta agttacgcgg ctgatccggt
     3841 gaacatcctc tcgcaatcgc tacataataa gaaagtaatt gaagcggaat tacatttaaa
     3901 attggactca agaattcatg acaaggtgga acttcgaaac aataatcaga aagtgattta
     3961 gcttctacat ctccctcggt tacaatactg atgatctttc cattacgagc ctttatctct
     4021 tggatgttag aaatcatctt aggataaatc tctgatttgt tggcaataca aaccactgga
     4081 acttcatttg tgatgagtgc aattggaccg tgtttaaatt ctcctcctgc atatccagaa
     4141 gcatgaatat aagaaatttc ctttaacttc agtgctcctt ctaaagcgat aggatgatta
     4201 taggttcttc ctaagaatac aaagtctttt gtattaataa aatcacttgc ccatttttct
     4261 aactcaggtg ctttatttag aataaattca atttttgttg gaagttgttt gatttcttcg
     4321 actaattctt ttcttagctc atcagacatc atccatttga tacttgaaag ataaattgcg
     4381 aaaagaagta aatgtaatac ttgagcagta aaagcttttg tgctcgcaac tccaatttcc
     4441 attcctgcac acgtatcaat aaatgcgtca gactctcttg cgatagtaga gttgacgtta
     4501 ttcaccattg ataagacttt tagaaatttt gcttttgctt catgaatgct tgccaatgta
     4561 tccgcagttt ctccagactg agaaattgct atgatcaatg tatcaccctc aacaacggga
     4621 ttacgatatc tgaactcaga ggacatttct gtgtctgttt gaatctttgt aaactgttct
     4681 aaatagtgct taccaatcat tccagaatgt aaactagttc cagctgcttg aattacgatt
     4741 cgatttactt ttgaaagtat ttccttactt aattccattt cagaaaaatc taaatcacca
     4801 ttttcattga ttcttctttc gattactttt cggaaaatac ctgcttgctc atagatttct
     4861 tttaacatgt aatgagggaa accagcttta tccacatctt cccattttgc ttcttgtttt
     4921 ttcatttctg gagcaactga atttccatca aaatcgaaaa gttctaaatc ttcttttgtg
     4981 aagtatcccc attgttttgg ttttagatag catacttcag aggagtttct gactaaagcg
     5041 gacaagtcag aagctaagta gtattctttt tttccttttc cgatgagtaa gggagcgccg
     5101 tcttgtgcaa agtaaacttt ttcaggctct ttttcaaaaa caactgcaat cgcccattta
     5161 ccttgaatca ttttaaaaag acttaaaaag gcttctttta tggatttttt ttggctaagc
     5221 tccatagaga gcaaatgcga aatcacttct gtatcagtac tgctttggaa aatatatcca
     5281 agtgattgca attttgtttt taattgcatg tagttttcta taataccatt gtgcacaact
     5341 gcaatagtct gtttttcgtt ggtatggggg tgtgcattga tctgtgatgg ttccccatgg
     5401 gttgcccaac gtgtatgtcc aacacccaca tttcctctaa caggtttttc ttttagcaaa
     5461 gcttctagtg ctttaatctt acctttttct tttcggattt caagttcatc attatcgatg
     5521 actgctatcc cggcagaatc gtagcctcga tattctaaac aagccaagcc aaccattaat
     5581 acagattcaa cactcttatt tcctgcataa ccaactattc cacacatata tttaactctc
     5641 tattctctgt tttgtttttg taagtaacgc ttgtaaatca ttctcatttt ttgcttctgc
     5701 aatgattcga atgattggtt ctgtgttaga agcacgaaca tgtacccaag atgattcgaa
     5761 atcaaatcga attccatctt tttcatttat tttgctgcct ttaaaattgt ccttcaattt
     5821 ttctaaaata tcttttagat ttcttccttg gacagatatg ctttcttttt gcatataaag
     5881 ttttgggatt ttatccatct cggtatcaac agaagatttg ttggaggcaa gaaaatttaa
     5941 aatatgacct attccagcta aggaatctcg tccaaaagag cttatatttg gatcaatgac
     6001 cccgccattg ccttctccac caaaaaaagc ctttttttga atcatttcac tgaccacatt
     6061 tgcttctcct acctttgcaa gatgaacctt tttcccataa cgggcaatgg cttcttgggt
     6121 gaggaaactg gtagatagat tgactaccat atctttgcct tttcttccta gaactgattg
     6181 gacacttaag gggagggtgt attcctctga tattacgcca cgctttggtg ttaatacaat
     6241 gagtctatct gcatcaggat ccaatgcgaa tccgatctct gcatccgttt ttttcattag
     6301 tttggcagtt ttgatcagag cttttggagt aggctcaggt ggacgcggaa atgtcccgtc
     6361 aggcgtgcaa taagtttcat ataccttaca gcctaatctc ttcagaaatt cgggaattac
     6421 caagcttccg ccgccattga cagcatctac aaatacctta aatttccttt ttttaatctt
     6481 tgccaaatct atttgcttta gaacagattc aaagtgaaga tcaattaaac tttcagatga
     6541 attggatttt atcgatttag gagaaaattt tggaagtggc aagtctccct tctgaataaa
     6601 atctttcaat tcttgaattt gtttcgcatc gaaaaagaag ccattttttc ccacaaattt
     6661 aaatgcattc cattcgagtg gattgtgaga ggctgtaatg atgattcctc catcactttt
     6721 tgtttctttt acaattgatt ttagtgttgg agttgggaca atacctattg tcttaacatt
     6781 aatacctata cctagtagaa caccagatac aatattttct atatattccc cactgggtct
     6841 agaatctcta ccaatgataa ttgtttttgg ttttaagaca tttgcgtaag caagtgtaaa
     6901 ttgaatgatg ttttcgaagt tgagacctgt ggggataatg cctcttatcc ctgaaacaga
     6961 aatcatcaat gaattggaat tattgattga aatttctcgg tagttcatat taaatttttt
     7021 aaaaatttaa taaatagaac atgggcgatg acaggattga actgccgacc cgctgcttgt
     7081 aaggcagctg ctctcccagc tgagctaatc gccctaagaa tatattccag aaaaaataaa
     7141 cttaagattt tgtcgagcga aattataaaa aacttagtga tatttttttt gaagttcgca
     7201 gacgaagcaa tgtttctaat tacttaattt attccttaat tttttatccc tgctatcttt
     7261 tttgaaagtc tgcttttgct tcgatccgct gtctttttat gaatgatctt gttttttgca
     7321 gctttatcga ttaaagaaga atactcttta taaaaattca ttgcatcttc tttttttcca
     7381 gcctttacag actttaatac gctttttgca aaagttctga tagcagattt tcttttagag
     7441 tttctatctc ttcttttttc tgctcttcta atatcttttt ttgaggattt taaatttgcc
     7501 aatgtcgtta acctttacta aataattttc ctgattgtac ttgctatgct ttaactttaa
     7561 gcctctaagt caagataaaa tattttttta ggctttaggc gcacagagaa cggatttttt
     7621 ttctcatata aggaaaggag ggatattatg tatcctattt tgatttcatt agatgagcca
     7681 attctccatt tcctagtttc tgagaaaata gacctatcta tcttactgaa aatcggactt
     7741 ctaaaagaag agagttctga ttttcaatat tttcattcag aaaataaaaa aaatattcgt
     7801 ttaaatcttg agagaagtta ttttgtctta ttaaattctt tttgtgtgaa aaatcacagt
     7861 tatatgcata tagttgcagg ggaattaatt ttggaaataa ttttgcagat attatcttat
     7921 gaataaagac aatgaatata taaaagtaga atctatctca gaaattcaaa ataatctaaa
     7981 ggtggactta ttaaataaga agttccaaga taaggatgga aatcgatttg tggttcgatt
     8041 tggaaatgaa agtcggaaat tagaaattct acgtatcata acgaagtcag agttagagtc
     8101 gggacacatt ggcgaattgg aaactaaaaa tgaaccttct cacgaccata aaattgatct
     8161 tgaaaaggag agagaggtag aggagcaaaa acagaaagag aatctcagac aggctgacag
     8221 acaggaaatc aatagcaaat ttaatgaaga ttcaggtgat ggttttggtg acattgattt
     8281 aaatattgaa gcagttgata cggttcacaa aaaagtgcac gatcactcct tgataaatcc
     8341 tagatctgcc gcagatgaga gaagatatat atttgatttg ttaaaagcaa tcgataaaaa
     8401 caaagaccgt gtagtctcca ttttgacgaa tgtgcaaaac tctcgtattt atgaactcac
     8461 tggtgatcca tctgaaaata aaaatattat ctcaaatttt acaagggaat ttgaaggaga
     8521 tatttttaga atccttgaaa acctacaaaa caaattcaaa gaaatcactt ctttccctaa
     8581 gactactcat cattattcta tgaattatac agttttgcag aaagactttc tagctaagtt
     8641 agagaccgat aaagccaaat ttgattatat tctcagatgg gattttcagg agccaactct
     8701 tatactaata aaaaagtttg caaaaatggt tacagatttg ttaaatgtat taaataaaaa
     8761 ggaagaggat caaatcaaaa ctttaagctt tcaacaacag acattattta gagatgctaa
     8821 gattgccggt gtctattgtc tgaacgattt agactttcta aaaagaagtg tggaaagctg
     8881 gaaagaatct gccgtatagt atataaatat gcctgaacta tcaaacaaag aaatagaagc
     8941 cttactctta aaaattcgaa atgaatataa gacttatgca caagagaatc caaatatttt
     9001 taaattagcc ccttttgaag aacgatatac gcaaatccta agatcgagag gaagtttgga
     9061 gttattcttt aaggatgagt tactttttct agagcaatta aaagcaaagc ataaagaact
     9121 gaaagcaaga agagaggcaa gccaagctcc aaccattggt agaatcatgg aagaaaacga
     9181 aactcgtata ctcaagtata acaaaattga ttttcaccct atggcaaaaa acgaactcaa
     9241 atatttctat ggggcaattt ctaattttgt tgagacagat ttacagttgc tttatcaaat
     9301 ttttagagga actgctgaga tcaacagtat ccatgattcg attatacagc tcgaacgaat
     9361 tggactcaat cgaaaaggac aattgccttt ccgtatttcc gagcatatga aaattcttac
     9421 aagtgtgtca gtgaatcaag gtaagataga acaagatagt cagtcgatta tgaaagatgg
     9481 ttgtcttgca ctgaaacata tttcaatgta tttagaagat gctgaaaaaa ataaaaaaat
     9541 aaatatgaag cttatattta aatttaatga aaaagattat ccagaattat ttaaaaaata
     9601 taatggaatt agttttgata ctggagcaga aatgataatt gtaaattgtg caaatatcat
     9661 tactgatttt agaatggatt ccatagtagg attaaaacca agatgaagct atccattgtt
     9721 atcccatgct acaatgaaaa aaatacaatc caaaacatct tagaagttgt tagaaaagtt
     9781 cctattaaag atagggaaat tattcttgtt gatgactgct ctaaagatgg aaccagagaa
     9841 attttacaaa atcaattaaa aggtttagtt gataagttaa tatttcacga agtcaatcag
     9901 ggcaaagggg ctgcccttag gacaggtttt caagcagcga caggcgatat agtaatcgtg
     9961 caggatgctg atttggagta tgatccattt gaaataccta cagtcattga gccaatcgtg
    10021 caaggtaaag cggatgtcgt tttcggatct agatttctag gtgggggacc tcatcgagtt
    10081 gtttattatt ggcatcgact tggaaatatg gtattaacaa cattatcaaa tatgtttaca
    10141 aatatcaatc taaccgatat ggaaacttgc tacaaagcat tcaaacgaga tatcatccaa
    10201 tctataaaaa ttgaggaaaa tagatttggc tttgagccag aaataactgc taaaattgca
    10261 aagataaaag aaattagaat ctatgaagtt ggtatttcct attatggaag aacctatgca
    10321 gaaggtaaaa aaattggctg gaaagatggt gttcgtgcga tttggtgcat tttaaaatat
    10381 aacttatttt aagtgatctg agcgaacatt ataccaatta taaatactct gtttataatc
    10441 tttctaaata tctgtcccta aatgtttctg gtatttattc gaaatgagca cggaaatgta
    10501 taaatagctg gaaagagaat tttttgtttt gaaaagtctt gtatgctttc actctttaaa
    10561 tgagttttga atccttttaa agagtgtttt cgaatggata ccaaatagaa ttaaacgtta
    10621 ttctacctcg atctttattt tgatttcttt atcgggtgga gattctttat caaacggttg
    10681 tctatgctca aactcaacca tcccagagcc tgactctaga gctcgaatgt aaaagatttg
    10741 aaattcttta atactaactt ccttgtcttc ttcttttttc tttggttgaa tgattctagg
    10801 tttaaacggc tgctttaaat tatcagagtg cttgactaag atccaactat aaccagtcgc
    10861 catttttacg ggaagtttta ttccttctag tttccctttt ttcattttaa ttgtaatgtc
    10921 atctgattca tcttcaatcg tggtgatgtg attgactttt tttactatcc gtttttttga
    10981 atttgcggaa agtgcgttag ttgctagaaa actagcaact aataatgtta tgataatata
    11041 atttttcatt ctatgtcctt ttctactttt cattgcgggc tacaacccac atcgttccat
    11101 aaccaatatt tgcagatttg taatctatcc acatatagcc tttaattccc catttgtcac
    11161 cccatgaatt tttaataaga taggcattta aactatcgtc ccagcctaca atcgtaattg
    11221 catgatttac atcactcgca ctagatgttt tcactttttc attaaacaca ccgcctgtgt
    11281 aagcttgaaa atggtttgta acatatactg cacttgctag aggtccgtat ttacaaagag
    11341 cttgttttat ttcttttaca gttggtgttc tgtttccatt tgccacataa ccccacgcaa
    11401 ctgtttgata gcttgttgtt ttgtatggct tgcactgcat atcttttcct ttgtaaggtt
    11461 ctgctgattc atcaatcccg cttcttgaac cagatgaggt cggacctttc ataagtaagt
    11521 aatcgaaagc atcacctggc caaccaccac tagcacaact tccagcacgg gaacagttaa
    11581 caatctgttg ttctgacata tcaattgatt tattgttttg aattaaatag ctagattcga
    11641 aggctgccgc agttgtaaaa gcccaacagc ttccacaaga tccttgttgt ttgacagggg
    11701 ttactttccc ttctgctcgc caatcccaag ctttcaaatc aactgtgcac ttggatggat
    11761 tttttttagg aggaggtggt ggttcaatgt aacctttgtc ttctgtttgt tgatagggat
    11821 catcctcact ataatcttgc atatcagcaa attcgtcagg caatggatct tcttcactgt
    11881 aatcgaattc ttctgtttct tcagagtcat cacttgtgaa ttttaccttc tttttttgtt
    11941 tttttctttt agcttccatc ttgagacgct tttgtgcctc ttggtttact ttattatagt
    12001 caatggaacg cggtggtttg gtagatgcta tttgagaaat ttcatacttc attgcttctg
    12061 ttatacccac tttgaagcta tagtttttat cttttatttc ttgttcaagc ttgctcagtt
    12121 ttgcttttag atcatctagg ttttgtttgt ttgcatcctt ttgtttttgt aaaatatctt
    12181 tcatcgattc acttgtttga gaatgaatgt agagagtaga aaacaaaaaa gagaaaaggg
    12241 aaattgctag caggattttt ctaggttgta gtttgttctt tttcatgagc gaagattctg
    12301 tttattttta taagagagtc aagaaaaaaa tttcctaaaa acggtgaatc tgattcgggt
    12361 aaaactgatt catattttgc tttttgagaa tgagtaaaaa ttaatttgtt atcttttttg
    12421 ctaattgctc aatgcttttt aaatcacgga taacacttgc aactcttact ttcattgttt
    12481 cgattgtttc atcttgtcga ttcgagtcat tttttatatt ttctatttta ttagaaagaa
    12541 gttgagtaga ttgaattatt tccataagtc cagttgtcat acttgctaga ctattttttg
    12601 tgtgggttgc tagtttacca acttcagatg caacaatttc gaaacctttg ccggcatcac
    12661 ctgctctagc tgcttcaatc gaagcattta atgataacat atttgtttga tctgcaattt
    12721 tttttatcat atgaatgact tcattgatat tagtaatttt tttatttgtc gcaagaattt
    12781 gctcatacgc ttctttagcg gaattatttg ttttctgtga agaggtatga atcgaatgaa
    12841 aagaatccag tataaactgt atggacttac ttgtaacaga acttgtttct gaaataatat
    12901 cccaactttc tacagatgag atccaaccga tcgctccgat tgcatttcca tttttatttt
    12961 gaattggtat tgcagatcct acataagcga tttgaaatgc tgattcttct tttgactttt
    13021 tggtttttat aaatgtattt gtaagtagag cctttgatgt gattgtttcg tttggtaaaa
    13081 attcaccagt ttttaaattg aagggtagat ttccttccct gtaatgaatt atttttctat
    13141 ctttatctat taggatggtt gtgtcttttt tttctacaaa atgaaataga tactccgcca
    13201 aaaatattcc tttttcaagt aagtcttttt cttcttcctc tctgcttgcg attaattcat
    13261 tttgaatagt tgatagtata aaattcattt agtttcctta tttataatct agatcttgcg
    13321 tccagtcgga tgggagaata attattatcc gctaatatta tcggtcaatc tgtctttact
    13381 aaaagactat ttatcttaaa taccaagtgt aaatttttag attccatttg ggtggtcttt
    13441 cttaaatcat cctttatagt gttgaaaaaa ttatcctgct agcgatttat ttccaattat
    13501 gtcatcgatt taaaaattga gttgttctga gttattttat tatttaatca ttagaatttg
    13561 aaaataaaaa tctttttaaa tcaacgaccc aatcttatga aattcccttt atcggattat
    13621 caaatttgat gatctgaatt aggtgtatgc tcttaggtat ttaaaaaaag gagagtagta
    13681 aagatgatac ggattgtttc aaaagcaaac tcaaatttct aaaaatttac ttggaagcta
    13741 attcggattc ttatttaatt ttcagactga gttttggcac tcctaaagta attttgaaat
    13801 agaatagaat cgtcagattc ttataaaaga aatatgggaa tcgagcgata gcttgactaa
    13861 aaaaaagagt tgtaaaatct aatccttaat atggcaaaaa agaaatacgc taactccttt
    13921 actctgtttg ggtattctat atttcatcca cttaatttga tattattaac aaattgtatc
    13981 atatttttaa tgcagcttgg tgagaatatg actatgacat atttatttgc tctaactcca
    14041 aatttatttt tttctggtag ttattggcag attttcacct acggattctt acattctaca
    14101 ttttttgatc tgattccttt tcatatactt atgaacatgt atggatttta tatgcttggt
    14161 agatacttag atccaattat tggaaaggca aaactagctg gactctattt tgcatcacaa
    14221 ttaggtggtg gtatttttat tattggttat gcacttctta tgaaatactt agtgatgcct
    14281 gataatttag agctaatttt aaaaactcca acgttaggtg caagtggagc tgtattcgga
    14341 cttcttgctg tttttggact tctttatcca gaggaagaat tgatcctttt aatttttccg
    14401 gctcgtgcaa aaaatgctgt atttctcttg ttaattatag gttttatgtt tgaatatttt
    14461 tttaatgcac caatcagtaa tttaggtcat ttaggtggtg cgttagtagg ctttttattt
    14521 tattattcat ttcttcgtaa atcaaataat agaattgtat ttcgaattac atttaatcca
    14581 gatgaattag aaccacgtaa agaaattgca aacgatccat ttcgaaatca agttgaacag
    14641 aacctacgaa ttagacatca aataaaaaca atttcttctt tgaaagacaa ggaagaatat
    14701 cttcaacctt atcaaattcc aaatgcaaat atttgtcccc cctctactta taatacggaa
    14761 gatgaatttt gtttgagatg tgaatggttt ggaaattgta tgcttcgtaa atctaaaatg
    14821 gagaatgaat aatatgaaaa aaacatttca gaaaaaaatt ttactagttt tgattttgtt
    14881 ggggctaatt gcatttatcc cattgattag tgattcaatt gatatactga cggacgatga
    14941 taaggacggg attagtgatg cattggaaaa atatgcaggt cttgatccca aaaaagatga
    15001 gtgtgagcct aacggttgta gtggttttta tggagatgaa tatcttgtga tgatcctcga
    15061 tcagagtgga agtatggctg aaaaaattgg atcagataca agaatggaag cggcgaaacg
    15121 tgtttctata aaattgattg ataagtttcc tgccacagcg gcgggacttg gagtttattc
    15181 ttatggaact agcacaacga taaccggtga acccgcagag gatacatcag gttgtaaatc
    15241 ctttaatgaa ctacaatccc ctttcaagag attagacaga aaagaattaa aagaacatat
    15301 tagtaagatg cagccagacc aaagcactcc acttgctttt tcgcttttaa aatttagaga
    15361 atcaatctta gataaaaaag gcaaatttaa tttaattctt attacagacg gggaagaaag
    15421 ttgcaatggg aatccagtag aggaagcaaa aaaacttgta atgttgaata gtaagacttt
    15481 agccattaaa ctttccgtta ttggatttgg ggttgacgat aaaaccgctg aagaactaaa
    15541 aaaaatagca atcgcatccg aggggaccta tcaatctgtg gcatcggaag aagcgttaga
    15601 agatatattt cagaaaccca tcaaggatat tattgcaagc ttccaaggca ttctttgtct
    15661 tcacaaacag gtggataaat tattgttatg tgaacaaagt agagataata aattgagagc
    15721 tgctttcaat cgactcaatt ctccattgtc gagccctttc tcagatgagg ataaaaaaac
    15781 aattatcaaa tattatccgg aaacggaaaa atacattcaa gcgagaatac aagcttatac
    15841 taaaattaaa aaagaaggga cagagagtta cgttaaaaag attgatgagt tgagtaaaat
    15901 gattgtgtct cctgcagaca gaattaaaaa gaaataactt gattctcgtt gattttgcca
    15961 atgaaaatgt ttttaaattg aggcatctaa tttttttatg aatccatttt taacagtttt
    16021 aaagaactta tggaaaagaa aattaattcg ctttttattt atcttggcgg tcatttataa
    16081 acttgtcttc aatgttttta caggacaagt tctcattgaa aaaatttttc attctatctc
    16141 aacaggaaca gtatctctaa atgtaaaaag attttcatta tttttcggaa tcataattga
    16201 gaatataaaa ttatcttcag gctttgattt tgataataaa cctgttctaa ctgttgatag
    16261 acttgcggtt acttataatt taccgctttt gtttacaggt agattaaagc ttactgaaat
    16321 ctctctgaca aagcccgaat tacatatgtg gcaaaaaaat ggaaaatgga atgtagagac
    16381 tctatttgta agctctgagg aaaaaaagga ggaagagaaa aaagaagaat caacagaatc
    16441 ttctaatgaa ttatcccttc caattccagt gagtggctat ttaaacttct ttattcatga
    16501 tttaaaaata gaaatagaag cagagaaagg tgaaaaatac ttttatgcct tcttagataa
    16561 ttttagtttt tattttctac tcaatacaaa acgattttca aaacttcctt attctactaa
    16621 agcggtatct atcattgatg aactaaaaat agaactcaat ccaaaagaag agattagctt
    16681 atctatgaaa gataatatcg cagctctgca gacaaaaatg cgtttatctc ttttattaaa
    16741 taatgataat accggcgatg ttccaatttt aacttctaag ctagatattg gttctgataa
    16801 aattccgatt aaaatgaaaa caggagatgt tcagcctttt aattttaaaa ttggatatga
    16861 ccttaatttt attcccgaga aagacattgt tgaactaaag gatttaatct tttcgtttgc
    16921 gggggataag ttatttgaag gaaagggaac aattaaagat gctttaaaag aggatccgga
    16981 agttcaattt gaaattgtag aatcaaatat aaatttagat aatgtagagc ggataacgga
    17041 cactgttcca attataccgc ctatgaatct aagtggattt tttagcttaa gaccatttac
    17101 cgtaaatggg aaattgagtg atttacacgt tcacagtggt atcaatggga aaaaaatcgt
    17161 agtagaactt ttcagaaaaa tccatgatat gccggttatc gactttcaat tcgatgctga
    17221 tctgaatctt ctaacaaaag aagagtcttc ggatataaat attttaccgc aacttaaaag
    17281 atcaattata cacaatcttg aagggcaata caatggaata gttttgaaaa taaagggaga
    17341 agtttctcca aaagaaaaag ttgatttgtt tttagattta gatcgggtaa ttattgaaaa
    17401 atttgttcca gatgtattag gaagagctca tctttatata actgctaaag gtgataagct
    17461 ttcttttatg aatctcgacc ttaaagctgt tttaagtgga cttcgttata aattaggtag
    17521 aggtctttcg ggagtcaatc aagttgagtt aaatttgaaa acggcaattg attttaaaaa
    17581 ggattttcaa ttggaaactc tgaaagtaga gcctttgact tttgcagtta aaaatgaaaa
    17641 caaagaagtg gctgctcgta ttgattccgc aatgaactta gatatgaaaa atggaacaaa
    17701 agcggaaatt gataaattaa aattagaagc aaacattact catttaattc ctactgttcc
    17761 agtgtcgtta cgcggaacca ttgcaggact aaggcagagt ctcggaaata ttctcactct
    17821 aaatggcaaa gctagttata aagaagaagc gaaaaataaa attattaact taggtcttgg
    17881 ggcgagcttt ccagctttag aactaacgga tatgaatatt actggactta tcaatattgc
    17941 aaaggatgag aaagaaacgc ttaccattga taatataact ctctctgcat tccaaaataa
    18001 attcaaaggt ctttttaagg gaaatctttt taaacccaaa acggcaaatc ctccctttgg
    18061 agagtatgca ggagagataa atgggaatct cactctagag tcggacgtat ttcggtatgt
    18121 tttaaaaggt atgtccttta agggagattt agatattgat ataaatgtaa aagatcatct
    18181 catcagtgga gcccttaggt caaatgattc gtatatcgtt tataagacaa gtgaatgccc
    18241 aggcaaaggt tgtcaagtgt atgagttaaa cgggcttaag atgaatattc catttctaca
    18301 tgatatcaat gataagacga cagaagaaat gctagatggg aataaaacaa attttgtgaa
    18361 gacttatggg ttagatgctc caccaaatct ttcgattcag tcgatagtcg gaacacatcc
    18421 atctctcgag aatgctaaat tagattttgt aaaatcaatc agtggaagtc caggatttgt
    18481 agctcgtgtt gattacatag aaaacttttt gacaattgat aatatgaaag cttactcaat
    18541 gaatggatat atttatggca aggatatatt gtttaatatt gggaatggag atccagctca
    18601 gatcgaatat tcagcagtaa tacaggtgag agatattgac ttgtcagaac tccttcccaa
    18661 agatatgaga aagaaaatcg atgatggaaa gattaaactt gatctgaata tctctggtca
    18721 aaatttgaat gatcctattg aaaatttgga attgtttttt agcacatttt atatcggaga
    18781 agattttggt aaaagtgcaa ttcgaattgt gagtccagag aatgtgatta cagaccaaat
    18841 tataaaaagt taccgagtag ataaaattga attggaactt acaaaaggtc tcgtgtatgc
    18901 cacaattaaa tttcaaaaat cagtttttaa tacactcgtt ttcaatgtgg aaaatgatat
    18961 gatttcacaa gagcgaattc cactcgctag ctttttaaaa cgaacagaaa aagaattatc
    19021 taattataaa tgagggttga atgtatacta gactttatat ttttccaatt ttaatcttaa
    19081 tgaactgcac atttctttct ttaagacccc cagctattac ttttacacaa tcacaaacag
    19141 ccgcagaacg tcagatgata ggagaaggaa aagatttaga gaaagatggg tggatcattt
    19201 cttccattcg cacatctgct tctggatccg atgtttggga aaaggaaatt ttagataaag
    19261 aaattccgga aggggaattg gatgaggtaa cctataccgc attaaagaga cttgcttatc
    19321 tatcaaagga tgtgcgagat tttaaaaaga aagactttat aggagaggct cttgatgggc
    19381 aaatcaaaat taacccctta ttaaatgagt ctagattcaa aaagcaattt cctgaaagta
    19441 aaaaacaaat agatgaatta attcaaatgg taaatgaatc taggcgaatt gtgtatgaaa
    19501 gtaaattgca aaagcttaat agtatgaatt tgaaagaaca agagttaata aaaaagaaag
    19561 acgctcttca gttatcgtat taccaattag ttgaggatgg tgaatactac gaagcaaagc
    19621 gcggtaagtg gggacgtaag gaataatgat gattttctca ttctttcaga aaaaatacaa
    19681 tggatggaat caaatccgac tttgtttttt atttattttt tttgcgaatt gctccctttt
    19741 gacttataga gtaaatgaag taaaaccaaa tgaatttcaa tatgaaaata taagtaaagc
    19801 tcattttact cctaataatg agaaaccata tccactaacg gttcgtcgtg gaaataatat
    19861 ttataattct acaacaaagg atgctcgttt tttgtttttt gcaagtgatg caaatgggaa
    19921 ttttgatatt tatatgcgag atttgcagag ttcggctgtt ctactcgtaa ctagtcaccc
    19981 ggctcctcag tataaaccag caatcagtcc agacggaaag agactcgctt tcgtttcaga
    20041 gaggtatgat tcagaaggtg atatagtttt gatagatata aatcccgaga agatgatcga
    20101 ttcttaccta agtggaagtt atgaagttga aaattttaag caggatgtaa ttacaaatcc
    20161 caatcacaaa gaaatttcga aaattgaaag agtcatggat tcagacccag attggtctcc
    20221 agatggaaga tatattgcat ttgctacaaa acgatttggt gggaataacc cagatattgg
    20281 aatcattgat accaagaata ataattcaat tactcgaatt acaacaaaag gaggggctac
    20341 tcctcgtttt gctccagatg gtaaaacaat ctattatatc tcttttcaag atgaattaat
    20401 gggagaaata tattccatcg atataaacac gaaagaagaa aaaagaatca caaaagataa
    20461 atacatggat ttcagtccaa cagtatcttc tgatggcaag tatttatatt atacatcaat
    20521 tagagtcgat acaaattgta ataaaaagtt agacgaaaga gacaatagct ttattattcg
    20581 aaaagaccta actactggtg aatctattca gctaacggct ggtaacctct ctctatttga
    20641 tacaagatat tcaaatttaa atggaggatc tattctattc agtgcgtctc ttgagaatgc
    20701 tctgaatata tattttattc caaaagatgg agaaatccca aaacaagaaa cgattacaaa
    20761 acaatatgaa ttagcccgtt tgtataaatc gagatcaatg gactatttca aattggcatt
    20821 taattctatg tatctttttt ttggtcaaga tccactcttt cctctgtatc gatctagagc
    20881 agataagcaa attgcaattg attatttaga ggatggagaa gatacaaaag gtgaaaatct
    20941 tcttaaagaa atgttaaaaa caaagggaga caaaaatcag gggctctctt atgcactttc
    21001 tattgttcag caaggtatta taaaaaataa gaatccaatt ccggaactaa cgtcctatta
    21061 tgaggaaatg aaaaacgaga aaggtgttca tccgcaaatg cttccttctt tgctcatgac
    21121 cattgcagat tattataaag aaataaacga tataagtatg gcagaaaaga cataccaaac
    21181 tatcagatta gaatatccca atttttatcg aataatggaa gtaaaaagaa aggaaggata
    21241 tttcgaatat caaatgaatc cgaataaaat tccagatagt tttatagaaa taatcaacaa
    21301 tccaaattcc aatcgagaag ataaaaaatt tgtgatggat gatatcatta aaatgttggg
    21361 tggacaggat gatttagaga aaaagtcgag gctaatcgat gtttatctca aagagcagaa
    21421 attagaccaa gtatgtccgg aattagctgg tttgctctat tatacgagag cagttatttt
    21481 aaatgaacag gataaattcg aagaaagtat caaattagta aattcctatt taactaatat
    21541 caataaaggg tcttatgttt atctaaaaag taaaatctta caatcagaca attatcgaga
    21601 tattaaagat gaagctaagt cgaatcaaga acttgtagac tttgtaaaaa attatgatta
    21661 tttttctggg gttgaaatta cagaagaaga aatggaaaga tctttttact atttcgaaac
    21721 aagagcaaga gagtatgaaa gaaaaggtga attaaaagaa tcgattcaaa attttgttta
    21781 caataactta cttttatccc ttgcgattga aaagaaatta ccgattcaaa aaacctatca
    21841 ggatttttct gttttctatg aaaaaatgat ggtggataca tcatttaatc tatcaagata
    21901 tgttaaagac aaagacttaa agaatatcat aaataaattt aatttattag gaaggagaca
    21961 gctagatgtt gccggtcgaa caactcgctc cttagcttat ttttttagat ggaaatattt
    22021 tagaatattt ggtgacttca gagacttaca gctgttattc cctatggagg aagacgcctt
    22081 tcagcagtca gaagaatatt ttaaaactcg tttgaaagtt gcgagagaaa atctagagta
    22141 tgcaactatc tatggatatg catattattt agtttcaaaa gcagtccaat ctgaaactta
    22201 tttcatcaat gaggatgctc ttactcgtgc aagaaaggct aagattctaa atcaattaaa
    22261 gcaagcagag tatgatttgc aatggattat ctatgcaaac ccaaattaca cagatgctta
    22321 tttacttctt ggatggctct atcagtatat agatgtaaga aaaaaaggat tggtctatcc
    22381 agaaaatgaa actgattctg aagtgtttga atcactttat gcaaaatttt ttccatacaa
    22441 atatttagaa gagaatattt ctctttattc gcaaatactt caatttcttg gacccaatta
    22501 tgaaaataaa aaagcgttat ctgatataca tttaaaccta gcaaataact attttctact
    22561 ttccaattat ccaaaagcat ttactgaata ccaagaagtg gaaaatctat ctaagtttat
    22621 tttagaaaag aatcgcttcg aaaattatag acaaaaggct gtttattatt ttaatcttgc
    22681 gcgtattcat gtttataatg gaaaaataaa agaatccatt ccttacttga aaaatgcttt
    22741 agaattgtat tataaaaatg aatacttccc tttgcttgct aaaattggaa caaaaaatat
    22801 tccagaatta aacagctatc tagatgatgt aaaaaagaaa atcgcattac tcaatgcact
    22861 tctcggtctt gtgcaaatgg aattgcgtat gtattcggat gcaattacat ccttttcaac
    22921 tgccgtttcc atgaatggca aatcagatta tataaatgat ataagccttt ataattctct
    22981 cgctttttgt tatcaagaat taggcgatta caagaagtcg gagactagtt taaaattagc
    23041 agataaagaa tatagtcgta agaagcgaag actggctgaa atattttcct tctcgattgg
    23101 cgataaattt tggagtatag cgttacccga cagtgctcgt gtgattggag aaggtagatt
    23161 ccctggagag attccactcg atttcgaaaa tctaattaca agaggagtta gaattgcaaa
    23221 ctatatagaa aaaaatgaat tcgatgaagt atctagacaa atcgaagata gaagtaattt
    23281 tataaaagag agaaaattgg attcgactct gattggggaa aagatattaa acaacaggct
    23341 caatgaactt ggattcaatg aattttctag aggcaattat ttcgaagcta caaaaatcta
    23401 tacggaagag tatgaagatt taaagaagaa aggtaaatta aaggaggctt ttcaatctta
    23461 tataaaatct gatattgcac tctattctca cattgaacaa aatgcagaga aaaaagaaaa
    23521 cctactaaaa gagttaaata cgaatattaa atttctaaat aaattcaaaa cagaagaaat
    23581 tctatcttgt ttgaaaaatt ataaattcga agaaaatacg acagacgaaa tgaaggaaga
    23641 gatttgtcga aatcaatttt atactgatta ttataactat gatccatatc ttggaaacaa
    23701 ttacttttat gcagcagaaa tctatctaac aagtaaagaa tacgaaaaag catttacata
    23761 ttacggaatg actcttcctc tttttaagaa tccttccgga ttgcaggatg atgaaattgg
    23821 tttagaaaaa gatttattta cgagtaaaca aagaactaga ttacggtttc ttacggctat
    23881 cgtttactta cgaattggtg aaacagaaaa atttgaaaaa attctgagag aaagttacta
    23941 tttagcgaat gaatttcaat tagaaaaaga attaatcagt tactatattc tagaagcaga
    24001 atacaacttt aaaatgggtc gatataaaaa atccattgag ttttgtgaga gagcagaaaa
    24061 ttccctaaag tcaagtccag ggctttggta tgaaattgat gaaatttcaa ttaataatat
    24121 ttactcactt aaatctgcaa atttagtaaa gttaaagaat tacacagatc tagcatcgaa
    24181 tagagaaaaa ttatttagtg cgattttctt tagacaactc atgatcaatg aattgcgttt
    24241 tcaggataaa gagatattta aaacgattaa taatttacaa ctatatgttt gggaagacaa
    24301 agagttaatt tataaaattg aagcaatcaa tttagcaaaa ggtgatccaa aagaagttgt
    24361 aactgcaaaa gctaagaatc tagaaaatat tctctatgaa atgaaaaatt tagaaaaaat
    24421 tttaccgaat ggaatggatg cactgagttg gtattctaaa ccgaaaaaat ttattccaaa
    24481 actaaaatcg gatgaattac tcatcagcta ttacactact ggattagatg gaacacaggt
    24541 catcctaaat agtaataaca atatcgaatt caatgatttt aagttaaatg ggaagagaga
    24601 tacggaaact ttttcgagag agctggaagg aatattacag cattatccgc aagttaagaa
    24661 attagttatt attccttctc cgcttatgta tgatattaat tttagattgc ttccatacaa
    24721 aggaaagccg ttaaacgaaa catatgaagt aagacattta ttccgactaa gtcaattaga
    24781 aagggaggcg gatgtagagt tttctcgact caagcgaatt accagtgtga atccaaacat
    24841 taaggtagat acagaaaagg gcatcgattt aaatccaatt aatttagttc ttccaaaagt
    24901 tgaacctaaa aaggatatga aggaattaaa ccttcgtatc attcaatcga atgagttaaa
    24961 aagttattta acagacacag atattttaga aggaccgact gattttagca atcgaaaaca
    25021 atatatcggg gaaaaaagac ccggtatgat tagtatgaaa gatgtggtag aagaccaatg
    25081 gaccataccg tttattattt taacaaatta tacaaagagt atggataatt atataaagac
    25141 tggattttta tatgatattc ttcaattttc tggtgttcaa tcgatcattc tattagagcc
    25201 tacagcggct aacgcaaaaa taagagataa tttgatttct aatattaaga acgccaataa
    25261 aataataaag gaagaaaaac tccatttagt tggtgaatat ataaatgaat atcctgagaa
    25321 tcagaggata tttgaaagtg agtttaaaaa atatactagt cttgctgtaa aagaagagag
    25381 aaaaagaaat tttcttaaat ctatgaaata cttattgcag gcaaattctg tattaccaga
    25441 aaataacgct gaatatctaa ttcgctctga gttaaactta gctaagttaa agacaaagac
    25501 atttccaagt gtttctaact atttagttca ttatgaaaat ttgctcaatc gatttgaaat
    25561 caattcaaga gaggaggagg caattctata cgaactatta attagctgct atgaggctcc
    25621 tattgcagtg aattgcgata aatactttag tcgttataat gctctttcca atgcaacgga
    25681 tgagaaaaag tatgttgtca attactataa aaatttgaga gaaggtaatc tcaatataat
    25741 cgatccagat tatgataaat ttatatcgat taaaacagaa gaagatcctt atcttaaaaa
    25801 tatgagactt gcatcgttat tcgcaagagg ttttatctgg gataaagcaa gacaacatgc
    25861 agagattgca aagaaattag cagaatccaa acaagaggta aattatgctg ataatgctat
    25921 ctcggatatt gagtatgaaa tattctttat taaaggaatt gatccagata cgattcgaga
    25981 ggataaaatt tatttttatg caacaaatcg aatttgggat gcttatcgca aaaaagcaag
    26041 agagcttgct gaaaatcaat cagatgcttc taggagaacc tatcaaagca gattattaga
    26101 tgcatatgac tccatcgaga ataaagccga tttcgagcct acaactcttg gaccgcttta
    26161 cttaaaagat ggaagacctg ctctttattt attaaaagaa tccgaaagag actttttatt
    26221 tcacttactt ttgaaatcga ttataacgca aacaggtgaa gagttaaata accagtttga
    26281 tatactcatt gaaacagaac ttcgtttaag aaatcgaaac cgtgctcttt ggatgcagat
    26341 ccaatgggcc ggtgcgttat accatagagg agattatgaa agtgcgaaga gatatttcaa
    26401 tgagtttgat aaaaatttcc gtgattatta tccagagaaa agtcttgtta aaacatttta
    26461 tacgcttaaa tataaattgt ctcgcgtatt tcccgagatt gaatttgcaa caagcgaaag
    26521 agattttatt aaagaagaat ttaaagagtg gtttggctat tatgaaatat gtgaacagaa
    26581 acagccaatc gattttttac cgattttaaa tcagcttgta gcttcaaaga aaaaagagaa
    26641 attagattta tttaatgcaa gagaattaga tgattttatt agtttcttgc aattagaatc
    26701 tattgaaaag aaagactatg ccgtattagc agatttaggt tttgcaaagg ataaatataa
    26761 atcaattaat gataaagtat taggtagaca ggtattattt tcagatctac cagaatttaa
    26821 gtctatctct agagcgttag ttgcaaaatt accaaaggat caaatattct cagcagttat
    26881 agatctagga attaagtctt atgttgttcg aatgaaagag ggaacgatca caaatgagtt
    26941 aggatttaac gataaccgag atataaaata ctcgatactc gattattatt acacaattaa
    27001 agatggagga gtttctatcg ttaagcaaga atcgatagaa gcaagatata aagaaatttt
    27061 aaaattttca aaaaataaaa ttgattatat ttatcttcct tcctatcact ttaaagcagt
    27121 catagaaccg gatgagcaag attattttta ttatgtatta agtcctgagc ttcttttaga
    27181 aagaccaatc tatgatacga ctcaggactt tgttccaggc tttaaggtgg aggctaaaaa
    27241 tatttcaacc tattctccaa aatggtggag aatgattcaa gaattagaaa agtatgaatt
    27301 gcgaaatgtc tctggagtgg gtaagggaaa tagaatccaa atatcacagg aagatttact
    27361 tttagaaaat ggtcgcaagc ttcaatttgc aaatatgggg ttaaatgaga ttaagcgaat
    27421 agaaaaaaga aagggtgtgt gggtaatgac tggtaacctc ttgtctgaat cttctgtgca
    27481 aaatgatgat tttccgcatt cgttacggta tatagatagc attcatcaag gaccgggtgt
    27541 agtgtttgcg agtcttcata accagacagc aacagctttt ttcttaaaaa atttctttaa
    27601 gaatttaaac tatcgttatc cgtttataga aagatactat gatgctttta cgacaacaca
    27661 ggaagagttc ccatttgata aatattggaa tggatttaga ccttatacaa atgtgtttat
    27721 aaaatagttt tatgaattta gttaggagat aaactatgga atttactatt agacctattg
    27781 gattgattcg ttcggagagg ttggaatcga ttgacgatca ttggaatcaa gttagatctt
    27841 ttattgaatt aacagacgaa tattctagtg agagtattcg tggactagaa gatttttctc
    27901 acatagaaat tgtgtattac tttcataaag taaaagcgag tcagattgtt ataaaagcgg
    27961 agcatccaag ggaaaatcca aattatccgc tagtaggaat atttgcacag cgaaagaaat
    28021 ctagaccaaa tttactcggt ctaactattt cagaaattga taaaatcgaa gacaaaaaaa
    28081 tatttctaaa gagctgtgat gcaattgatg gaacccctgt cattgatatc aaaccagtgt
    28141 ttaaagaatt tcttccgaaa acagaaatta agcagccagt ttgggtttcc gatttaatga
    28201 aagactattg ggaataaaga gtgaatcgaa gtgtgtaagt attttcttcg ccaatggtga
    28261 agactgataa atgaatgata aagaaacgat ggatttagaa tttatcctat ctttcctatg
    28321 cgctcatcgt ccctatcggt ggtaatcttc taaaatattt ctttatcgaa attgcattac
    28381 ttaaataact ttgcattttt atgaacgctt tgtcttccca gttcaatatt tccaatgcca
    28441 atatgaagtt tgtctggatc gactgcgggg gtaatggttg ttatcctgtg gatctttgtt
    28501 ttatatttct tagctaataa atcttttagg tctcttgttt cctcttcaat cactgaaagt
    28561 ccttggctga cagcgcgtcc taaaatatat ttagattctt cttgttcttt atggacgtta
    28621 tgtgtgatga ttgtatgaat cgatttgtct aagatcaatt cttttgttgg ctcgtgatca
    28681 atcaatcctc catccgtgta atccatacca tttatttttt gaacctcaaa gagtaatgga
    28741 aaagcactgg aggcaagaat tgcatcgatt gcatctcctt tagtaataaa ttctcttttt
    28801 cgtttggtta gattgactgt agcagctccg aatcgaatcg gaaaatcttc tatctttttg
    28861 tttccaagga atggatgtaa aaaattcttt agtttaacac ctgttagctg tccactgtaa
    28921 ttcttgaaac catgtcgaaa tggttttata agatgagaaa aaatatttcc ttcccagaag
    28981 tcttttttct taaattcttt gatgatagaa atcatttcat ctatattgac tccacttgca
    29041 taaagggcac caactatcgc accagcagag caaccagaaa caaaggaagg tttaaatttt
    29101 agttcagaga ttccctttac aaaacctgca tgtgcataaa acccgaaaaa ggcagagttg
    29161 aggcaaatgg ctgtttgttt ttttgattgc atagaatacg gataggggct gtgtaaaaac
    29221 tttaaaaaaa atctaaccac gaagagcacg aagatcgcga aggtcaaaat ttcctaggaa
    29281 attttcaact tttcttcgtg gccttcgtgc attcccgccc atcgcttgac gctcacaata
    29341 gccaagctat ttgggtgaaa atcttgttct tacacaaccc ctaaagaatc aaaaaaattt
    29401 ctaatacttc gtcttcgtta atggaagctt aacgtttaaa atcacttgaa ctcgtgtgac
    29461 ttcaccttct tttactttga tgattgaatt gtttaatttc aaatcctctg caaaagaagc
    29521 cttcacttca tagtcacctg gaattagatt tgtaaaccaa aaatttcctt cactatcagt
    29581 tagaactttt tgaacattgt tcacgctctt tactgtaggc atataaattg gttgtttgat
    29641 gactggattg tccaaagttt tataaaacac atttccttga atcactccga aaccagacat
    29701 agcatttgta agttctagaa cattgtcctt attttccaaa actgcgaatg ttgttacaac
    29761 ttcaggatac tcatctgctt tgatggtaat cttatgaacc ccagtcgcta ctttggagac
    29821 tcgaatatga tagatttctc cttgaatgag tcttccacct cttacaacgg atccccagaa
    29881 atcagacttt attggaatga gtttgaaatt tacttctttg tcatcgatta atactttaac
    29941 tcttctatca ccctctctta cttttttttg cttcatcatc tcttcagggg gtaatgtgga
    30001 taagccgtaa gctctatctc gaatgattgt agtcttgatg agtaaatcac cgttagttgt
    30061 tacgttgatg acaggaggct cagcaggagt aacaggtggc tgaactggat ctggtttcac
    30121 aggatccgaa gcaggttcag gtttaggctc tggttttggt tctggcttag gatcaacagg
    30181 aggaggtggg atcacagggg ttacattccc tgctaagaag ataccacttg catctcctga
    30241 gacttgtgga acttgtttgt gatcgttgtc ttctgccatt ttaatcacag catctctaga
    30301 tttaaaaaat gcctctaatg ctgttactac tttatctcca tttaaatcgc cactttctaa
    30361 agcgcgacca aattgatatg taaagattcc atgattgata tcaccaccaa cctcaattgc
    30421 tgtttggttg tcatccgcag aagaaataat tgctttgttt tgaaaataaa aatcttctgg
    30481 atcttgtcta acggttcctt ggcttccatc tggaattgga acatctttgt ctccacgagt
    30541 tgcctttcct tttttcgcaa ttccaccgga gaagcagcaa tcaaaaacaa aaacagtctt
    30601 ttgtgttttg attgttttta gataatcatt taattcatca tcggataggt gaggtctgtc
    30661 atagcagacg agataatttc gcataccgtt ttttgcagag gcatctcttt gaaagaaacc
    30721 atgacctgcg aaaaataaga acacaacgtc acttgtagtc gctttcgctc caatggcttt
    30781 tatttctctt tcaatatttg cctttgtgat ttgggagccg agtagaactt tgatttcatc
    30841 gaagttcccg acttttttga tttgatcatt gatatacgta gcgtcagctt cgcaaagatt
    30901 caactcactg ataccagctt tatttccctt gtagttgctt cctaaaatta atccatatcg
    30961 ctttgcgaat agattggaag aagagaaaac gagtaaagac aaaaacaaaa taattttaat
    31021 cttcattgga tctcctttat gtggtgaaac gcgcattatg cgcttgaact tactattttc
    31081 gcattgaatg tttttttacc acggataaac acagattagc acggatggac taatttccta
    31141 aatcttttat cggtgttcat ccgtgttcat cggtggtaat ctttcttttt acttagctag
    31201 ccacttcatc atgctgcgga gttttttacc aaccgcttcg attggatgcg cagcaccttt
    31261 gtcgcgtaac ttttggaagt ttggatagcc agcttttgtt tctgcaaccc actctttcgc
    31321 aaatttcgca cctttgtctt tttgaatttc atttagaact tctttcattc tcgcttttgt
    31381 gccagcgtca atgatacgtg gtcctcgggt taaatcaccg tattccgctg tatcagaaat
    31441 agaaaatctc atacgagcaa gtccaccttc atagatcaag tcagtgatga gttttacttc
    31501 atgcaaacat tcaaaataag cgatttcagg atcgtaacct gcttcggtta acgtttcaaa
    31561 accagcttgg atgagtgcag cgagtccacc gcaaagaact acttgttctc cgaagaggtc
    31621 agtctctgtt tcttctctaa agctagtttc tagaattccc gctctaccac caccaactcc
    31681 ggcagcgtgt gcgagtgctc tcttgtgtgc agttcctgtt gcatcttggt gaattgcaat
    31741 taaacaagga actccaccac cttctgtata aactctacga actaaatgtc caggaccttt
    31801 tggggctacc atgtaaacat ctatgtcttt aggagggttg attaaatcat agtgaatgtt
    31861 aaatccatgc gaaaacacga ttgcatttcc tggttgtaaa tttggttgaa tgtctttttc
    31921 gtaagttgct gcttgcactg tatcaggcgc taaaatctgg atgatgtctg ctttttttgc
    31981 tgcttccgca atggagtaaa cttcaaaacc agcttcttta gcttctttga tggatttaga
    32041 tccatctttg agtccgatga tgacttttaa acctgagtcc ttcatgtttt gagcttgtgc
    32101 atgtccttga cttccatagc caattactgc aattgttttg ccttgtaaaa cgcttagatc
    32161 gcaatctgcg tcgtaatata attttgccat tgtttccttc gtgaatattg aattttacag
    32221 tcattatctt aggtaaactt agaatctcaa ccttaaaata gatcggttct tatgtcgttt
    32281 gggcgttgcg gcgatttcac agtagtaggg aacgcatata tgcgttcact acaaagatta
    32341 acccgccgcc gcgctctcac tctggtcctg ttttttctga cttcgatacg ccattaagaa
    32401 atggctactc agtcaccgga aaaaacatga ccccgttcga tcgctaacgc ataggagttg
    32461 tgaagttttg aactattcta aaccaaacct cttcgcaaac tctttttttt gtttccaaag
    32521 tctgctcgat ttgtctataa tccaacagaa aataaatcga tttttagtaa gttcgaatta
    32581 cacttgctta tgatagaaac atcattcctt cttgctaaaa ataaaaatac aaaaattatt
    32641 atagaatgat tgaattgatt gcaggtgatt tactagaaca agatgtagat gtaatcgtca
    32701 atccatggaa ccagaatttt ataaaatatg acaaaaaaag atgagccgat tttctattgc
    32761 gaaacatgtg gtcgagcagg agactatctt gattttgttg acttgagcga tgaggaaatt
    32821 gatccctttt ctatctacac agtttgttcg gaatgtttaa acggcatgcg aaagaatgaa
    32881 ttagtaggga ttattttgtt agttctttat ttaatcagtg gtatttttgt tgggctagaa
    32941 gttaagaatc aattgaatct tctctttgtt atttatagtc tgaattttat tagaagaatt
    33001 ttgtatttta gatttttaaa aaataagata agaagagata ataagagtca
//
LOCUS       DAOQOS010000057        32780 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_601_length_32780_cov_5.270833, whole genome shotgun sequence.
ACCESSION   DAOQOS010000057 DAOQOS010000000
VERSION     DAOQOS010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 32780)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32780)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32780
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_601_length_32780_cov_5.270833"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..435
                     /locus_tag="PLF66_13560"
     CDS             <1..435
                     /locus_tag="PLF66_13560"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HMZ65120.1"
                     /translation="AYLAGILHDITKQKTKDFHREIFIRNDFSYNDLPEAAYHPFSAV
                     FYLKEKYLFTDVEILSAIEKHTLGNENLSLLDEILYVSDFLGSDYAKRQPEYQNWILE
                     TEKQLSFGVILKSSKVIEELIEKKFRIHPLTVFMYNKYLNIH"
     gene            510..824
                     /locus_tag="PLF66_13565"
     CDS             510..824
                     /locus_tag="PLF66_13565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65121.1"
                     /translation="MKKIIFSILVSMISLSSVWAIDEFEKLMVKTADTPEEKKAIYKY
                     LMKEAENKRKMAEKFKEMANVSKGGKAITQINHKKEMLEEAKSLEESAVEYEELAKKV
                     KE"
     gene            complement(1376..1849)
                     /locus_tag="PLF66_13570"
     CDS             complement(1376..1849)
                     /locus_tag="PLF66_13570"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HMZ65122.1"
                     /translation="MNRFSLIIFISLLGFQMTQCSSGDVKGDPSLSIVNDLNKQLETL
                     EIEGFEGNKTTLSDKLYKEWEPKGIPVLKEIIPKIPEGYALKVTGHADPYGGEEAAKK
                     IADGRAKHIRDRVAKALGIEGSKLETKNYGANEYEQEKNQSISKNRRVEFQIYKQ"
     gene            2157..2675
                     /locus_tag="PLF66_13575"
     CDS             2157..2675
                     /locus_tag="PLF66_13575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65123.1"
                     /translation="MSSKRFSVERKQDRVIYTYDRYDHFSFILIGFSAYFIVDFLINF
                     KPEKYALDSINMDSVFGIISLLVPAYLFYSIINSGFNSTILEINYDSVIISESPIPIS
                     FTKKILRKDIVKIFTSSESFENDGRWEQHTIFLQLADNTELPVFKYITDLSECKTMEA
                     MIRRDLFSSELD"
     gene            3088..4455
                     /locus_tag="PLF66_13580"
     CDS             3088..4455
                     /locus_tag="PLF66_13580"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HMZ65124.1"
                     /translation="MKVFFPLQKKFKTRGLFQLLFFLLDLGSKKASPEDQIRIRITNL
                     ISLSFILGIFIFTFMQLILFSLEEMPFLISFGILNLIILLLNGLGFSSYTKGLHIIII
                     NLSIFVIVQKEKDLPAIVVYQLLAAFLPFLLFRWNQIKYIIISILFNFALVILSSPYL
                     FSWLTPNTHTKEQIVYETAFALWNSYVCSICFLLYFYVQNEKMKRRVASQTLRTKNLN
                     AQLTELLEEQKLTTQIIAHDLRNPLSSILGIHSILNPILKSNQELPQQISKFIDLIFK
                     SAENGIRVIEDIQDAYSFEQKDTTIEIHSFVLEDLLNEMKSNFLQKIQEKQLHFIISC
                     PTGLRLKTNRLRLSRAIENALTNAIKFTPVQGDIFIKVFTKDYIDITIQDTGIGIPKK
                     LHRYLFQKFTQAKRRGIQNEASTGLGMYIIHKIMTSLGGTVTVDSIENQGTSISLLLP
                     LSNME"
     gene            complement(4491..5258)
                     /locus_tag="PLF66_13585"
     CDS             complement(4491..5258)
                     /locus_tag="PLF66_13585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010410626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HMZ65125.1"
                     /translation="MKLITLNCNGIRSAKTKGLFDYLKKSKADILAFQEIKASREDID
                     LDFFHKLGYSEFIFPAEKKGYSGTAIFSLQKPKSHEIGIGNKLFDSEGRNILVEFKNF
                     AFINTYFPSGTSGEERQAVKMDFLDFYLKKKSEWEKKYKKLIICGDVNIAHTEKDIHN
                     PKGNEKNSGFLPEERKWVTDFLQSGMVDAFRAANPDKLHAYSWWTYRFNARANNKGWR
                     IDYFFVTKNLEKKIQSCFIDASLVVSDHAPVILEMDI"
     gene            5301..6587
                     /gene="hisD"
                     /locus_tag="PLF66_13590"
     CDS             5301..6587
                     /gene="hisD"
                     /locus_tag="PLF66_13590"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010574549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HMZ65126.1"
                     /translation="MAIKIKKISIKNESEYSEILKRAKGDISEALNVVIPILEDVKKN
                     GDAAIRRYTEKFDSLSLNEFKFETKDLEAKIPEDWKKALENAKSNIRQFHAAQFKTNM
                     EVEVSGNRLGIKYTPIESVAVYAPGGKALYPSSVLMGAIPAKIAGVKKVSLITPPSKD
                     GNLNPVLLYAAQISGVDTIYTIGGAQGIAGLAYGTETIRKAEFIVGPGNRFVTAAKSY
                     LAGIGQIGIESPAGPSEVLIIADDSANPSWVACDMLSQAEHGEDSVAILVTDSEKLSL
                     EVSKELEKALSERPKRKEIKSKAIYDNSIILIFPTIEEGIEFSNLYAPEHLEIITRNH
                     KKDFEKILHAGSVFLGPYSPVAMGDYISGTNHVLPTAGGSRIYSSLGVDTFLKRVTYQ
                     EIQKDSLKKLYPFVKTLSEIEGLDEEHGTSVYLRTL"
     gene            6686..7012
                     /locus_tag="PLF66_13595"
     CDS             6686..7012
                     /locus_tag="PLF66_13595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012388725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HMZ65127.1"
                     /translation="MELKFREKGKHKIIRLIGSMDIYTASKIKKEITQIIDDEECESV
                     IFDMSEVHHMDSSGIALLANIQKKMKSNGNKFGLLSPTNDIMGVLKLSSLDTFFTIYQ
                     SDLQIS"
     gene            complement(7040..7121)
                     /locus_tag="PLF66_13600"
     tRNA            complement(7040..7121)
                     /locus_tag="PLF66_13600"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7085..7087),aa:Leu,seq:tag)
     gene            7177..9240
                     /locus_tag="PLF66_13605"
     CDS             7177..9240
                     /locus_tag="PLF66_13605"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HMZ65128.1"
                     /translation="MTSHTSKTNSETRVIIVEDDPTILMHLEFMLKNKGYQVVGNATN
                     SVDALLVIREKNPSIILMDVSIEGDIDGIDTALIIKEEFNIPVVFITSFFDEATIARA
                     KIATPYGYLIKPVAPKDLYIAIDISLYNHSMERQLKENEEWFYTSLGSINDGVITIDN
                     NSNIKFINPSAEKLLHLTSSVVGKSLHDVYIPIREKMQKENPKSDYLLMHDRTENLYL
                     LQSDGSKIFLDETVRPIQDTHLGKSLGKIIVFKDITKRLALEKKVMDRLRYEIGLGLF
                     SRVLLSPFTQLSNLNSTFKELLSYIGLTRIVFSSISQVGYETVFSVIEEATVDEVSKV
                     GIGFPTDFQQFIHLQENVLKENNLIYGNIKNAPDKLKSTLSLREIKSYIFIPIFYQHT
                     LDGFIFLEDAVSIREWPEEDLQIFRIIGDLISTFIERTRNENLIKNHRDYLEKLVEEK
                     TSELQEAVKLAQAANKAKSEFLASMSHELRTPLNSIIGFSKLIKLPEGYEKEKEFITY
                     INSAGNHLLKIVNDILDISKMESGKTVMIKTKFSLYDSLISSIYIMKPQASKKNMTVI
                     QPEQTDLNLMGDEKKIRQVFINFLSNAIKFTGENGIIEINLKEINGFAEISFKDNGVG
                     ISLEHQKFIFDKFYQVGQVMYSENEGTGLGLSICKHIIENHGGVILLDSTPGKGSKFT
                     IRLPL"
     gene            complement(9235..9612)
                     /locus_tag="PLF66_13610"
     CDS             complement(9235..9612)
                     /locus_tag="PLF66_13610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65129.1"
                     /translation="MLTFFSILFTFIFIGMVLMIFYNSEDKRLDQQRKERELKEDNFQ
                     GADPKKVYGKNWDKNIKRPRICPICGTGLKKTEFLYAYMEDSFGPSGRRPVHIYGCKF
                     CYLGQVSDSSQEEINKVSDTLDL"
     gene            complement(9614..10291)
                     /locus_tag="PLF66_13615"
     CDS             complement(9614..10291)
                     /locus_tag="PLF66_13615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003001738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65130.1"
                     /translation="MGIRRFQNHAIIKEFNGLKKFFRAHETSVSRRRIGDFKRFVSLI
                     ESSGDTIGFDLMGSVNFGQATEHSDTDVVMYLHCDTGRIGECDNENCTRLNLYKNLLI
                     NTLVYEYSNNTYPVQVVDCINLNQLDYDLKIRNYNSLALVKFGFYRSICRGVNRKLLY
                     QYEQRLEDDNELCDVIESSLADCFLGLIHSSQHSYSFKKYIERVEDGGFKVPETMAKK
                     INSYLSY"
     gene            complement(10291..11298)
                     /locus_tag="PLF66_13620"
     CDS             complement(10291..11298)
                     /locus_tag="PLF66_13620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020766603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HMZ65131.1"
                     /translation="MKIIYLTDIHDGLKGLKNVFQQTTPNLYLLSGDIIYKAFFSTDL
                     IIEFCTYQEELEILAKNAGEDITPYDYATRVIRFSNKYSEEIQQSCVRYRELFNKAAK
                     TMKEKYQIIEKLIKNYAGSPCILLPGNYDIDLQYTALYELDIHRKSMEYGGLKFSGYG
                     GAPISTSGIPQKLAVKFHEYNKYGHSYSEPEDFFKEELPDIAMIHDPAYGFLDKIPGF
                     GNIGSQGIRRYLDEAEKKPSLVVSGHVHEDQGILKKGKTVFLNPSNFGAVDSVYGFQP
                     GGFFAEITIENKLVSSVGLYRLTDGVKNHLMEINTTEKVLKAEYVMENSPVSEEEFVR
                     K"
     gene            complement(11299..12051)
                     /locus_tag="PLF66_13625"
     CDS             complement(11299..12051)
                     /locus_tag="PLF66_13625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65132.1"
                     /translation="MIEYFVRRATRKIGQVMKTTEQIMTTPILDLFQPEKSIDILLNA
                     ADEQLKEDYKVSLNLRYINELIYIQVVEETEHLDTFTIDSNKGKVYLEGNFITRYYPL
                     AKFFRIESVNYKVELTPIWVKNNHVRFRISNFQIWDSRPRRFDIVRFISSFDFFHKRY
                     IIQTIAKLFPNFLSLTRLNNEIRVNLNYFLNKVNPAHNIEIKKLSAHNHKILFQVRSN
                     IIIKSLMDMFGIDIVVVEPIQDTDSFINEYGI"
     gene            12153..13676
                     /locus_tag="PLF66_13630"
     CDS             12153..13676
                     /locus_tag="PLF66_13630"
                     /inference="COORDINATES: protein motif:HMM:NF012457.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HMZ65133.1"
                     /translation="MKKIIHSLSIFLIVLAILSCGKERKKQLMTDFSSVQETMQFPKD
                     FLFGTSTAAEQMEETPNSDWGKFIQDAYSNKRFETMGHGIAKPGHINNLGSYSTDHIL
                     KKTNHNELYEKDFQMAKQMKHNAYRFSVAWDRIFPRPNMKEPDREGILYYKKLISSMK
                     ANGLYPLLTLFHFSSPAWFWEEKNGKVGWERDDALEQYEKFLRVVVDNFAEDVDHWCT
                     LNEPMVFILGGYMQGVFPPLQTRKEITDIIPVMSRLLHAHALSRKIIKESEKRTGKKN
                     LIGYTMHTRAFEPFRNYAPLDRIISEKVEQAFIWDFIDAATTGVLKVTNTDYKEEIPG
                     LKDSLDYLGINYYGRYYIKSNIFSPTKFETLFSDPDTKREIVNDLDWEAYPLGFSYIL
                     STAYSKYKLPLYILESGTADREDNDIKRQANIVTHLAEIWNLIHTKKADVRGYIHWSL
                     IDNFEWAEGFEAKFGLVKVEYHNNYKRTPRASAAVYTEIIEKGISKELLEKYSKDYK"
     gene            complement(13900..14625)
                     /locus_tag="PLF66_13635"
     CDS             complement(13900..14625)
                     /locus_tag="PLF66_13635"
                     /inference="COORDINATES: protein motif:HMM:TIGR02206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02206 family membrane protein"
                     /protein_id="HMZ65134.1"
                     /translation="MNLFNFYLLEPRFEMYSLSHIIAISLTFLSIILLYLFQKRIQEN
                     QRIEILLRYLIVSILASGEILLQYWYMATNEWKIEHGLPLQLCDFSIFLCIILLIRKS
                     FSIFEIVYFTGVGGALVAILSPELWLGFPHFRFYHFFLSHAAILFANFYMIWIYQFRP
                     NLKSILKVFFFINGFGVFVFFINSMIDANYMFISKPTYNNTFLNHLGPYPYYLISLEG
                     IMIIICFILYIPFRIRDYLTPLK"
     gene            14848..16353
                     /locus_tag="PLF66_13640"
                     /pseudo
     CDS             14848..16353
                     /locus_tag="PLF66_13640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004503938.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
     gene            16615..17025
                     /locus_tag="PLF66_13645"
     CDS             16615..17025
                     /locus_tag="PLF66_13645"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HMZ65135.1"
                     /translation="MDYKAIGILKGNTITLETEIPIRGKVTVVIQQETENVTQHQILS
                     FLKENISECRNLYRIEKIGIFGSLARNEQTSDSDIDIIIKFSENPKNVFNSKNKIRES
                     IEEKFKKKVDLANEKYLKSHVRDQILKEAIYDIE"
     gene            17062..17856
                     /locus_tag="PLF66_13650"
     CDS             17062..17856
                     /locus_tag="PLF66_13650"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HMZ65136.1"
                     /translation="MKKVILILFCISLSLHAEAFTVGEVLKYNISLWGITVGYSTMSV
                     SGKTLVNGEECFIFETKAEGTPFINRIFKVDDRIKSIWNIPRKLPVYSEKKLHEGFYK
                     RNHSIVFDLKKKTAHWEQNQFSGNTDKAGVQKQGAKWVEKEGDYANLPSEFQDILSAI
                     YYQREYKAQVEVGKSFSISLFDDLKLTEMKIQILREEKLELKINGEEKEIDTIVVQPF
                     IKTTGIFRSKGNIYIWISNDSKRMPVKITADIPFAGHVTVLLKEVI"
     gene            18015..19490
                     /locus_tag="PLF66_13655"
     CDS             18015..19490
                     /locus_tag="PLF66_13655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65137.1"
                     /translation="MLVVETMDVKHNDYPSIKDIDQNILKNRINRNISQRNQWKDMSY
                     FFFLNLIITILFTVSIFPDEVVRKNGTVISNVKATIKRQTIRIEYENGRIEETSKKEF
                     KAVRVRPIVWSSLLTSLTIEQKKIEEALEKERVAEASADGSDWEFRPEEEQISPWKNA
                     ALGLIPGYSGLYLTKNYWSAGIFTALETVALANFLDVANAQKTSPNIVEFHQAHNITG
                     WILTSGIGGYFRSRATNSNSPMLASENLSYGFIPTYVLYNHTYKGYKGGLSGREISPI
                     AVEGESNRLHAQEIQYASALLGGILIVDGILSYFSASAWNEGIYTGPKSQKPTTRLSR
                     VSRSVFFPGWGQIYGGETIKGYSYLTVGLALIGNIILAESNVANTYRKYQDAPFILYP
                     IYAVYAFQNNPVPAYGASNLLAKDKQEGLIEAVEKRNQAWTYFGVFWAWNLLDAAFLS
                     GVNKIDNKIRITPNISYRNNLGVGNRLSLETYYTLNLTLEF"
     gene            19492..20694
                     /locus_tag="PLF66_13660"
     CDS             19492..20694
                     /locus_tag="PLF66_13660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65138.1"
                     /translation="MKKIISIVFILFVLNCRNNEVAAAKQNILDPANPISYSLFKLSS
                     DLIGSSSSSCSSCGAAVSSNQPNSLIGFSDRIIIPSESNSSTNPSVWVLSDGINFTKF
                     TVKIGDCVGAFSSTSSTVCVPRLVGYENGMYAVYGQKVTNSSGQQTIAAEYYGTGSNL
                     NTINLTTTSPGGNANINSTFLYSAGKFHFYGCANNQCALYTTSDGSSWIASVGFSCEL
                     IYLADNGSPTCGLSRYFNGSTWVNAGTISGGANSSYSALYIDNIYRAWRNNSGTLSIF
                     NSTSGTITTGITFPGSANSTVSMNGVTNSPSQLAKTSVGVYLMVTSFFSNNVSTFIWL
                     RSSDGNTWTSYNPSIPTDLGTLSNLFGYAALGSSFYTVAQFKSGTNFNNYLLKSSDGL
                     NWTRVTLP"
     gene            complement(20784..23609)
                     /gene="uvrA"
                     /locus_tag="PLF66_13665"
     CDS             complement(20784..23609)
                     /gene="uvrA"
                     /locus_tag="PLF66_13665"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001156251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HMZ65139.1"
                     /translation="MADSFIKIRGAREHNLKNINVDIPRDKLVVITGLSGSGKSSLAF
                     DTIYAEGQRRYVESLSSYARQFLGQMEKPDLDLIEGLSPAISIEQKTTHRNPRSTVGT
                     VTEIYDYLRLLYARLGKPHCPVCDAEISSKSVDQIVEQVEEFPEGTKIQILAPLVREQ
                     KGEHKDVLERIKKDGFNRVRINGEIKTLDEEIVLKKTFKSTIEIVVDRLVMKKGLGSR
                     LADSIETALKQSEGTLIIEDGKKDHTFSQKLACPNNHEISFPELSPRIFSFNSPQGAC
                     QTCDGLGSLLEFEEELIVNDPALSLMEGCLEVWGKSQGYWYIATVHSLAKSLKFDYNT
                     PWEKLPKNIKKIILYGDKSIKIDYDFRKEDSHFEFSREYEGVIPNLKRRYKETKSDSM
                     RQWFETFMTNLPCPDCHGKRLKPESLAVRLHGLTIDKFTEMSVERALDFTAKLPIKGS
                     ESIIATPILKEINQRLTFLRDVGVGYLTLERSAGTLSGGEAQRIRLATQIGSKLMGVL
                     YILDEPSIGLHQRDNTKLINTLKDLRNLGNTVLVIEHDEETMIESDYILDLGPGAGVH
                     GGEIVAKGTPKEIMKDKNSVTGKYLSGKESIPMPKDRRKGNGNKLRIIEASHNNLKNV
                     TVDLPLGMLTVITGVSGSGKSTLINEILYNELAHKINKTKVISGKHKKIEGIQFIDKV
                     INIDQSAIGRTPRSNPATYTGLFTPIRELFANLEDAKLRGYSPGRFSFNVSGGRCETC
                     QGDGILKIEMHFLPDVYVTCDVCKGKRYNKETLEVKFKGKNIYEVLEMTIEEGCTFFE
                     NIPMVKRKLETLKEVGLDYIKLGQAATTFSGGEAQRIKLATELSKRPTGKTLYILDEP
                     TTGLHFDDVKKLMSVLQSLVDKGNSMIVIEHNLDVIKCADYIIDMGPEGGDGGGEIVA
                     MGTPEEIAKVSKSHTGQYLKKVLAKEK"
     gene            complement(23853..24395)
                     /locus_tag="PLF66_13670"
     CDS             complement(23853..24395)
                     /locus_tag="PLF66_13670"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HMZ65140.1"
                     /translation="MIYLAFNKPFQVLSQFKPLDDKKTLADYIRLPEKPKAVGRLDYD
                     SEGLLLLSNDIRFIQKMTDPDKEIEKEYLVQVEKIPTEEDLAKFKKGIRTQSESYKPA
                     IAILTDAPDFLWERNPPIRKREKIPTSWIRVIIREGKNRQVRKMTAAIGFPTLRLIRI
                     RIGNISLGNLKPGEYRKINI"
     gene            complement(24376..25623)
                     /locus_tag="PLF66_13675"
     CDS             complement(24376..25623)
                     /locus_tag="PLF66_13675"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HMZ65141.1"
                     /translation="MKNLIPIQLTNSTAMLVLGGHPWIFRNKFTRYEKSLKDGDWILL
                     IGAGNKELGVGIYAKTGLIAVRIIYFGENFSLEILQQKIQKRMEKRIPLLSQTDSIRW
                     IHGENDSLPGVTIDGHGNCLVIMCYAKSLESFARYIGRYLFYLFKKDNRIKLKPETIL
                     QSVPKRTGFETKEAVPRILRGIKTDWVKINYRSISYWIDPFGQKSGIYNDIRNLRDYI
                     LNHKEYFASKKILNLFSSNGLLSICLERAEAKEIISIDASRKAILVQEWNLRKEKDLK
                     LLTESETKIGNHRIIKLDIFKNLKETLEKEKTIYDVIIIDPPNLTASEKDKETARKIY
                     KKLIKETIPNLANKGTLILCSCSNRIHPNDFERICKEAISESGRKVKNPIRLKNEIDH
                     PILDTFPEGNYFKAHIYHDLSSL"
     gene            complement(25799..27088)
                     /locus_tag="PLF66_13680"
     CDS             complement(25799..27088)
                     /locus_tag="PLF66_13680"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020766739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HMZ65142.1"
                     /translation="MAEKAILKIGDKDYELPVIEGSEKEKAIDITKLRAQTGYVTIDS
                     GYLNTGACTSSITFLDGELGILRYRGIPIEQLAEKSTFTEVAYLLIYGKLPNHSELNV
                     WNESLTKHTLIHEDLKRLFSGFPKDAHPMAIMSCIIASLSTYYQDSYDPENPEHREIS
                     IRRLLAKFPTIAAFSYKKSIGQPAVHPKNSLDYCANFLNMMFSVPAEEYKIDAEIVKA
                     LNLLLILHADHEQNCSTSTVRLVGSSLANIYAAISSGICALWGPRHGGANQEVLEMLT
                     EIKQSGMSVKEVVTKAKDKNSTFRLSGFGHRVYKNFDPRAKIIKKACDSVLLKMGIKD
                     PLLDIAKELEEAALKDQYFVDRKLYPNVDFYSGIIYRALGIPTNMFTVMFAMGRLPGW
                     IAQWKEMIESPDMKIGRPRQIYVGPTEVSYEDAKKNA"
     gene            27229..29022
                     /locus_tag="PLF66_13685"
     CDS             27229..29022
                     /locus_tag="PLF66_13685"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HMZ65143.1"
                     /translation="MFIQNGIRIYLILSLLIFFPNCKQEKKNNLLPLGMLFLYSQSQA
                     RSSNCNYNDFTTDGSSDPYLSSQWYYSNSGEDLNPSSVWNSNRGERVRVAVVDDGLDI
                     YHEDLTSNVVPGGSYNFGVGYYGFSTSLSDPFSVVATHGTAVGGVIGARDKNGLGIAG
                     IAPRSCLVGYNVLVNSTDANTYTAMTYNASDVSVSNNSWGATDGNGKTAANSSLWASG
                     VDLGVTTGRNGKGTVYFWAAGNGANTNNATAASGVSAYPAGSLETDNSNYDGQANYYG
                     VAAVCALTDDGKRVNYSEKGANLWVCGYAQKTNYSTTQDGIWTTDITGYDGYNPLPSD
                     YLASGYSQSKQLSNYNYSKIFNGTSGATPMATATAALLVNANPNLGWRDVKLILAESA
                     RKNDPTDSDWLTNAGSKISSSGTPYNINHKYGFGVIDINRAVTLAKTWTNVGTMLQYA
                     TSTITANASFSNNTTLAAGASSSVNITSSNTSITKIEFIEITVTTASADASDLEIALT
                     SPQGTSSTLAEAHICSVGSNTYPSSTSQVGACGNYSGWVFGTSRHLGETPIGTWTLRV
                     ADRRSGTGSSSGWGNGGTLTSWKIKFYGRAN"
     gene            29023..29568
                     /locus_tag="PLF66_13690"
     CDS             29023..29568
                     /locus_tag="PLF66_13690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65144.1"
                     /translation="MKVFINNRSLYLVVIFLTSALSILAEPKALTWKEGEREHLIYLS
                     QDYIAEIDPPKSENKERNIRIIPISDTKLKSALAKGNLPSNYQGTHSEVFEDGNSRRK
                     MTLPGDIFVEFEESWTEKNVKDWADTENLILISKFPGIKNIYRFKTDPGIKSLNLANT
                     ILTKAGVKAAYPNWWREFVRR"
     gene            complement(29576..30412)
                     /locus_tag="PLF66_13695"
     CDS             complement(29576..30412)
                     /locus_tag="PLF66_13695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65145.1"
                     /translation="MKKSIIWNISNGVEFPVELWKHPKLAILINNVPLKEFKSIELNP
                     QEINLFFISAKQEEWSPIEYEFIKMFGSRSEISLLLLSQVGNTPIQINTANKGNLELL
                     ESPIRKKELKLIMEKTIQAEFYKTSAVEIGSGCLQNVGFFEGLFELAHAEYKNSQDII
                     HAFEKMLEYESKIKSYNNEVSQAIMRVNEMREAEMLTLVETIKANEKLDLLREKELKE
                     AIEIQEATEKALQYSRIEEMNMDKILRAHDRIFKYSEQEIKDLIKENIELKKKLGIKV
                     EE"
     gene            30664..30993
                     /locus_tag="PLF66_13700"
     CDS             30664..30993
                     /locus_tag="PLF66_13700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002771622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HMZ65146.1"
                     /translation="MVTILLLTVSNIFMTFAWYGHLKFKHLDLWKVILASWGIAFFEY
                     CLMVPANRVGHGRFNATELKTIQEAITLIVFSIFSILYLKEEFKWNYAVGFLLIVLAV
                     YIIFKKW"
     gene            31000..32382
                     /gene="murF"
                     /locus_tag="PLF66_13705"
     CDS             31000..32382
                     /gene="murF"
                     /locus_tag="PLF66_13705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004788621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HMZ65147.1"
                     /translation="MIETFNYPLFTIRSLFNDFRWTESERLKYITTSSSEVKNNSLFV
                     PLRGNRDGHEFIPDALKKGAISFLCEKDHPILKDLNFDEIQKAIIVDNTLYALGKLAT
                     FHRNRFQPFIIGITGSSGKTTTKELMGLVSESIGKSNVVITEKNYNNEIGVPFTLFRI
                     NEKTKIVVCEMGMNHLKEISRLTAMVKPSIGMITNVGPCHIENLGSLENIARAKAELI
                     EGMPPKSILYVPESILHRNIFQEKAKEYKVILKSFSLNKNPNLEILERKTDGFLLNLF
                     GTSVHWKIPGDKILENLSGVLAIAKEIHFSSEQIKKQLSLFKAKDKRFVVEKGYFHLI
                     NDTYNANPDSMESSLSSLKQISEGKNYFAILGDMKELGKFSKKYHEQVGLFCRKLGLS
                     GLITYGADAKWIQKEFSSNKNNISNSHFDYESIDALVEYAKKNIPKGSYVLVKGSRSM
                     KMERIVEGLK"
     gene            32383..>32780
                     /gene="purE"
                     /locus_tag="PLF66_13710"
     CDS             32383..>32780
                     /gene="purE"
                     /locus_tag="PLF66_13710"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020768763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HMZ65148.1"
                     /translation="MKKKNSPIVGVIMGSFSDYETMKEACNILERFHISYEKKIVSAH
                     RSPDVMFDYAKTAVKRGIKVIIAGAGGSAHLPGMVASLTTLPVLGVPIQSKALSGLDS
                     LYSIVQMPGGIPVGTLAIGNAGAINSALLAV"
BASE COUNT        10892 a         5216 c         5678 g        10994 t
ORIGIN      
        1 gcttacttag ctggaatatt acacgatatt acaaaacaaa aaacaaaaga ttttcatagg
       61 gaaatattta taagaaatga cttttcgtat aacgatttac cagaggcagc ttatcatcct
      121 ttttctgcgg ttttctattt gaaagaaaaa tatctgttta cagatgtaga aattttatct
      181 gcaattgaaa agcatacctt aggtaatgaa aatttgtctc tcttagatga aattctttat
      241 gtatccgatt ttttaggatc tgattatgca aagaggcaac ctgaatatca aaattggatt
      301 ttagaaacag aaaaacaact atcgtttgga gtgatattaa aatcttcgaa agtgatagaa
      361 gaactgattg aaaagaaatt tcgtatccat ccacttacag tttttatgta caataaatat
      421 ctaaatatac attaaaaaaa gaaaagaatt taaacgaact tttattggtt cttgttgtct
      481 aaagaataat taaaacaata ggagtttgta tgaaaaaaat tattttttct attcttgtat
      541 cgatgattag tttgtcttct gtttgggcaa ttgatgagtt tgaaaagctt atggtaaaaa
      601 ccgctgacac accggaagaa aaaaaagcta tctataaata tttaatgaaa gaagctgaga
      661 acaaaagaaa aatggctgag aaatttaaag agatggcaaa tgtaagtaag ggcggaaaag
      721 caattacaca aatcaaccat aaaaaagaaa tgttagaaga agctaagtct ttagaagaaa
      781 gtgcagtaga atatgaagaa ttagctaaaa aagtaaaaga ataattcatt tcggcctgga
      841 ttggataccg ttttaatttt cttaaatgct tatcaaaaaa tccaggcatt cccacccgtt
      901 taaattctcc tttaaacgat acttcaatta agctagaggt aaatatcgat tattcgttcc
      961 ttctactact ttatctatta ttcttaaaga gaagtgtagt ctcatcttaa attgagtttt
     1021 cgtctattga taaaaaatta caaggcagct tgaatatgcg gtcttaaaaa atgaaaaaag
     1081 tatagataat ataagtatag ggaagcgtag gtcgcgacag ttttggaaaa aaagaaatag
     1141 ttagattgtt ttggtctaat tcctttttct cgtgaatata tattctgaat tctgctaagt
     1201 tttaatttta gtataaccaa ctcacacaaa tagaaaaaaa tatgcgaaca ggaatgatct
     1261 cagtttttta taaatcaagc aatcattcga tatattgtag cagtatggat cccaactaat
     1321 ttttacttcg aaaagaagtc gagagactca tactgacata aaatatttga ataacctatt
     1381 gtttgtagat ttgaaattct actcttcggt tttttgaaat agattgattc ttttcttgtt
     1441 cgtattcgtt agcaccgtaa ttttttgttt ctaacttgga accttcaatt cctaatgctt
     1501 tagcaactct atctcgaatg tgcttggctc ttccgtctgc gatttttttg gctgcttctt
     1561 ctccgccata tggatcagca tgccctgtaa cttttaaagc gtatccttct ggaattttgg
     1621 gaatgatttc tttcagaact ggaatccctt taggttccca ctctttgtaa agtttgtcac
     1681 ttaacgttgt tttgtttcct tcgaaacctt caatctcaag agtctctaat tgtttgttca
     1741 aatcatttac aatcgataaa ctagggtctc ctttaacgtc tccggaacta cactgtgtca
     1801 tttggaagcc taataaacta ataaaaataa taagtgaaaa tctattcatg tattctcctt
     1861 tatgtattta gacaggattt tggatgaaag tttatttttt tcttttttac ttataaaaaa
     1921 tttcaaacct ttatctcacg atttaagttc gtctcatatt tacaaaaaga atcaaaattg
     1981 gttttaagga tttcatttat aatattagaa ttgtaaatta aaaaagtata aaaaaggata
     2041 gaaagaaatt acttaaatct ctaagaaata aaaacttacc actttaatag ggattttcag
     2101 cataatttta gttgtcttaa atctattcta aaaaatgatt ctttataagt agtaatatga
     2161 gttcgaaaag atttagtgtc gaaagaaagc aagaccgagt catttataca tatgaccgtt
     2221 atgatcattt ttcctttata ttgataggat tctctgcgta ttttattgtt gatttcttaa
     2281 ttaattttaa gccggagaag tatgcattgg attcaataaa tatggacagt gtttttggta
     2341 taatatcctt actcgtccct gcttatttat tttactccat tataaattct gggttcaatt
     2401 ctactattct tgaaataaat tatgactctg tgattatttc cgaatctccc attccaattt
     2461 ctttcactaa aaaaatttta agaaaagata tagtcaaaat atttacaagt tctgagtcat
     2521 tcgaaaatga tggacgatgg gaacagcata caatattcct tcaactagcg gataatacag
     2581 aattacctgt cttcaaatac attacagatt taagtgaatg taaaacaatg gaagcaatga
     2641 tcagaagaga tttgtttagc tcagagctgg attagagaag aatatcttat acctgtggtt
     2701 tcttaaaaca ggctttgaca acctgtttaa aaaactagat tctgtgtgat cgataaactc
     2761 tatataacat actgaatcct gcggattttg cggggaaaat attcattaca caaataacta
     2821 cgcgatcaga tgtgataccg attggagtgt taaaatgtgt ttcggtttcc atttcgtcta
     2881 tggttctaga agttgcttgg taactatccg atatgcgaaa gatagcagaa gaatttattc
     2941 tctttggcag tgtttgatag gcggacatct aatatttatt ggaaggacat cctaaattta
     3001 tacgctttct gcttcttaat cagctaaagt aagttaaaat aattttgctt tccttattct
     3061 gccgtctaat tagggtaaga gatcacgatg aaagtatttt tcccattaca aaaaaagttt
     3121 aagacacgag gtttgtttca acttttgttt tttttgttag atcttggttc caaaaaagca
     3181 agtccggaag atcaaattcg aattcgaatt acaaatttaa tttctttatc ctttatactt
     3241 ggaattttta tttttacatt catgcagttg attttatttt ctctagagga gatgcctttc
     3301 ttaatttcct ttggaatttt aaatctaatc attttactat tgaatggttt gggatttagt
     3361 tcttatacga aaggcttaca tataattata atcaatcttt ccattttcgt aattgttcaa
     3421 aaagaaaaag atttacccgc tattgtagta tatcaattat tagccgcatt tcttccattt
     3481 ttgttgttta gatggaatca aattaaatat ataataattt ctattttatt taattttgcc
     3541 ttagtgattt taagttcacc ttatcttttt tcatggctca ctccaaatac acacacaaaa
     3601 gaacaaattg tttatgaaac tgcttttgct ctctggaata gttatgtgtg ctcgatttgt
     3661 tttttattat atttctatgt tcaaaatgaa aaaatgaaac gtcgtgtggc atcccaaact
     3721 cttagaacta agaatttaaa tgcacaactc actgaattat tagaagaaca aaaactcact
     3781 actcaaatca ttgcgcatga cttacgtaat ccgctgagtt ccattttagg tattcattcc
     3841 attttaaatc cgatccttaa atcaaatcaa gaactccctc aacaaatttc taaatttatc
     3901 gatctaattt ttaaatcagc ggaaaacgga attcgagtga tagaagatat tcaagatgca
     3961 tactcatttg aacaaaaaga tacaactatt gaaatccatt cttttgtttt agaagattta
     4021 ttgaatgaaa tgaaatcaaa ttttcttcaa aaaatccaag aaaagcaatt gcatttcatt
     4081 atatcttgcc cgactggact tcggctaaag acaaatcggc ttcgtttatc aagagcgatc
     4141 gaaaatgcgc ttaccaatgc tattaagttt actccagtgc aaggagatat atttatcaag
     4201 gtatttacaa aagattatat tgatataacg attcaggata caggtattgg aatcccaaag
     4261 aaactacata gatatttatt ccaaaaattt acacaggcaa aaagaagagg tatacaaaat
     4321 gaagcttcta ctggacttgg gatgtacatc attcataaaa ttatgacttc tctcggtgga
     4381 accgtcactg tcgacagtat agaaaatcaa ggaacaagca ttagtctttt attgcctttg
     4441 tctaatatgg aatgaatttt ttaatgaata aaagacgtta tcctatatat ttatatatcc
     4501 atttctaaaa tcacaggtgc atggtctgag acaacaagac ttgcatcgat aaaacaggat
     4561 tgtatttttt tctctaaatt cttagtaaca aaaaaataat cgattctcca tcccttatta
     4621 ttagcacgtg cattgaatct gtaggtccac caggaataag catgtagctt atcaggatta
     4681 gccgctcgaa aggcatctac cattcctgat tgtaaaaagt ctgttaccca ttttctttct
     4741 tccggaagaa atccactgtt tttctcattg ccttttggat tgtgaatatc tttctctgta
     4801 tgtgcaatat taacatctcc gcagataatt aattttttat atttcttttc ccattctgat
     4861 ttctttttta aatagaaatc taaaaaatcc attttcacag cttgacgttc ctcgccggaa
     4921 gttccggaag ggaaatacgt atttataaag gcaaagtttt taaactcaac caatatattt
     4981 ctaccttctg aatcaaatag tttattgccg attccgattt catgagattt aggtttttgc
     5041 aaggaaaaga tagctgttcc actataacct tttttttcgg caggaaaaat aaattcgcta
     5101 taacccaatt tatgaaaaaa atccaaatca atatcttctc tagatgcttt aatttcttga
     5161 aaagcaagaa tatcggcttt actttttttc aaataatcaa agagtccttt tgtttttgcg
     5221 gagcgaattc cattacaatt cagtgtgatt agtttcataa attccttttc tcctaacatg
     5281 ctttctgttc cctagttatt atggctataa aaattaaaaa aattagtata aaaaacgaat
     5341 cagagtattc tgagatatta aaaagagcaa agggagatat ctctgaagct ttaaatgtag
     5401 taattccaat tctagaagac gtaaagaaaa atggagatgc ggctattcga cgttatactg
     5461 aaaaatttga ttccctttca ttaaatgagt tcaaatttga aacaaaagat ttagaagcaa
     5521 aaattccaga ggattggaag aaagctctag aaaatgcaaa gtcaaacata agacaatttc
     5581 atgctgctca gtttaaaact aatatggaag tagaagttag tggaaataga ttaggtataa
     5641 aatacactcc aattgaatct gtagcagttt atgcaccagg tggcaaagct ctctatcctt
     5701 cctctgtttt aatgggagca attcctgcaa aaatcgctgg agttaaaaaa gtctctctca
     5761 tcactccacc atcaaaggat ggtaatttaa atcctgtttt attatacgca gctcaaattt
     5821 ctggagtaga tacaatctat acgataggtg gagctcaagg gattgcagga ttagcttatg
     5881 gaacggaaac aattcgaaag gctgaattca tagtaggtcc aggaaataga tttgtaactg
     5941 ctgcaaagtc ttatttggca gggattggac agattggaat tgaaagccct gcgggtccaa
     6001 gcgaagtatt aattattgcc gatgatagtg caaatcctag ttgggttgcc tgtgatatgt
     6061 tatctcaagc agagcatgga gaagattccg ttgcaatact tgtgaccgat tcagaaaaat
     6121 tgtctttaga agtgagtaaa gaattggaaa aagcgttatc agaaaggcca aagagaaaag
     6181 aaataaaatc gaaagctatc tatgataaca gtattatatt gatttttcca acaatcgagg
     6241 agggaattga attttcaaac ctttacgcac cagaacattt agaaattatt acacgaaatc
     6301 ataaaaaaga ttttgaaaag attttacatg ctggttctgt attcttaggt ccatattcac
     6361 cagtagcaat gggagattat atttcaggaa caaatcatgt ccttccaact gctggaggaa
     6421 gtagaattta ttcatcactt ggggtagata ctttcttaaa aagagtaacg tatcaagaga
     6481 tacaaaaaga ttcattaaag aagctttatc catttgtaaa aacactttcg gaaattgaag
     6541 gcctagatga agagcatgga acttctgttt atctaagaac cttataaatg agaattctcc
     6601 atatgaaata aaaattttct ttacaaaatt tatatttcat atataaggtc tccattttca
     6661 aagcgtagtc atatttggag ggacaatgga gttaaaattt agagaaaaag gtaaacacaa
     6721 gattatccga ctgattggaa gtatggatat ctatacagcc tctaaaatta aaaaggaaat
     6781 aacgcaaatt attgacgatg aggagtgtga gtctgttata tttgatatgt ctgaagtgca
     6841 ccatatggat tcttccggaa tagcacttct agcgaatatt caaaagaaaa tgaaatccaa
     6901 tggaaataaa tttggactct tatctccaac aaatgatatt atgggagttt taaaattatc
     6961 ttccttagat acatttttta ctatctatca atcagatttg cagattagtt aatggaatta
     7021 aataaataga tgtttgactt gcgagtgaag ggacttgaac ccccacacct ttcggcacca
     7081 gaacctaaat ctggcgtgtc taccaatttc accacactcg catgatgcta taatttagga
     7141 tttgactttt atgtcaaatc ctaaatagat ttactcatga cttcccatac atcgaagacc
     7201 aattctgaaa caagagtcat tattgttgaa gatgatccta caattctaat gcatttagaa
     7261 tttatgctta aaaataaagg ctatcaagta gttggaaatg ctacgaatag tgtggatgca
     7321 ttattagtga ttcgggagaa gaatccgagc attatcttaa tggatgtatc aatcgagggg
     7381 gatatcgatg gaattgatac agccctcata attaaagaag agtttaatat tcctgtagtg
     7441 tttatcactt ccttttttga tgaagctaca atcgcaaggg ctaagattgc gactccctac
     7501 ggttatttaa ttaaaccggt tgcacctaaa gatctatata tagcaattga tatttctctc
     7561 tacaatcatt cgatggaacg tcagttgaaa gagaatgagg aatggtttta tactagtctt
     7621 ggaagtatta atgatggagt tataacgatt gataataata gtaatataaa gtttatcaat
     7681 ccttctgcag aaaagctttt gcatctaact tcttcagtcg ttggaaaatc tttacatgat
     7741 gtgtatattc caattagaga aaaaatgcag aaagaaaatc caaaatcaga ttatcttttg
     7801 atgcatgacc gtactgagaa tttataccta ttacaatcag atggttctaa aatatttcta
     7861 gatgaaactg ttcgccccat tcaagatact catctaggaa aatcactcgg gaagattatt
     7921 gtatttaaag atattacaaa aagattagct cttgagaaaa aggtaatgga tcgtttgcgt
     7981 tatgagatag gtttaggttt attttcaaga gttttacttt ctcctttcac ccaattatca
     8041 aatttaaatt ctacatttaa agaactttta tcctatattg gattgactcg tattgttttt
     8101 tctagtattt ctcaagttgg ttatgaaact gtcttctccg tgattgagga ggcaacagtt
     8161 gatgaagtta gtaaagtagg cataggattt ccaactgatt ttcaacaatt catacattta
     8221 caggaaaatg ttttaaaaga aaataatttg atctatggta atataaaaaa tgctcctgat
     8281 aaattaaaat caactctatc tcttagagaa attaaatctt atatttttat tccgattttt
     8341 tatcagcata ctttggatgg atttattttt ttagaagatg cagttagcat tcgtgaatgg
     8401 cccgaagaag atttacaaat ttttagaatt attggtgatt taatttcaac ctttatcgag
     8461 agaactcgaa atgaaaatct gataaagaac catcgagatt atttagaaaa gttagtagaa
     8521 gaaaagacaa gtgagttaca ggaagcagtc aaattagccc aggccgcaaa taaagctaag
     8581 agtgaatttc ttgcaagtat gagtcatgaa ctcagaactc cactgaattc tataattgga
     8641 ttttcaaaat taattaaact cccagaagga tatgaaaaag aaaaagaatt cataacctac
     8701 atcaacagtg ccggaaatca tctgcttaaa attgtaaatg atatattaga tatttctaaa
     8761 atggaatcag gaaaaactgt aatgataaaa acaaagtttt cgctttatga ttctttaatt
     8821 agttcaatct atataatgaa accccaagca tctaagaaaa atatgactgt gattcagccg
     8881 gaacaaactg atttaaatct aatgggagat gagaaaaaaa ttagacaagt gtttatcaat
     8941 ttcttatcaa acgctataaa atttacaggg gagaatggga tcatagagat taatctaaag
     9001 gaaataaatg gatttgcaga aatatcattt aaagacaatg gggttggaat tagtttagag
     9061 catcaaaagt ttatctttga taaattttac caagtaggac aggtcatgta ttctgaaaat
     9121 gaaggaacag gacttggtct ttccatttgc aaacatatca ttgaaaacca tggtggagtt
     9181 attttgcttg atagcactcc cggcaaagga tcaaagttta caatccgttt acctttataa
     9241 atctaaagta tctgatactt tgtttatttc ttcttggctg ctatcagaaa cttgtcccaa
     9301 gtaacagaac ttgcagccat aaatatggac aggtctacgt ccacttggac caaatgaatc
     9361 ttccatatat gcatacaaaa attctgtttt tttaagacct gtgccgcata tcggacagat
     9421 tcgaggtctc tttatatttt tatcccaatt tttcccataa acttttttgg gatcagctcc
     9481 ctgaaaattg tcttctttta attctctttc ttttctttgt tgatctaatc ttttatcttc
     9541 tgagttataa aaaatcataa gaaccatacc aatgaaaata aaagtaaaaa gaatagaaaa
     9601 aaaagtcagc atatcaataa ctcaaataag aattaatttt tttcgccatc gtttcaggaa
     9661 ctttaaaacc accatcttct actctttcaa tatatttttt aaaagaataa gaatgttgag
     9721 aggaatgaat taaacctaaa aagcagtcag ctaatgagct ttcgatcaca tcacataatt
     9781 cattatcatc ttcaagtctt tgttcatatt gatagagtag tttacgattt acacctctac
     9841 aaattgagcg ataaaaacca aatttaacaa gtgctagaga attgtaattt ctaatcttca
     9901 aatcataatc aagctggttt agatttatac aatctacaac ttgaacggga taggtattat
     9961 tagaatactc ataaactaac gtattgatta aaagattttt gtaaagattg aggcgtgtgc
    10021 aattttcgtt atcacattca ccgattcgtc ctgtgtcaca atggagatac ataacaacat
    10081 ccgtatctga atgctctgta gcttgaccaa aatttacaga tcccattaag tcaaatccaa
    10141 tcgtatctcc agacgactcg atgagggaga caaatcgttt aaaatcaccg atacgtcttc
    10201 tggaaacaga tgtttcatga gcgcgaaaaa atttctttaa tccgttaaac tcttttataa
    10261 tcgcatgatt ttggaatctt cgaataccca ttatttcctc acaaactctt cttctgaaac
    10321 aggtgaattt tccataacat actcagcctt tagaactttt tcagttgtat tgatttccat
    10381 aagatgattt tttacaccat ccgttaagcg atataaccca acagatgaaa ccagtttatt
    10441 ttctattgta atttctgcaa aaaatccgcc tggttgaaaa ccataaacag aatcaaccgc
    10501 tccaaaattg gaaggattta gaaaaacagt tttccccttt tttagaattc cttggtcttc
    10561 atgcacgtgt cctgaaacaa ctaaactagg ctttttttct gcttcatcta aatatctacg
    10621 aatgccttgg gatccaatat ttccaaatcc aggaatttta tctaagaatc cataagcagg
    10681 atcgtgaatc atggcaatgt caggcaattc ctctttaaag aaatcttctg gttcactgta
    10741 agaatgacca tatttattgt attcatgaaa tttaactgca agcttttgag gaattcctga
    10801 agtggaaata ggtgcgcctc cataaccaga aaattttagt ccgccatact ccattgattt
    10861 tctatgaata tccaattcat ataaagcagt gtattgcaga tcaatgtcat aatttcctgg
    10921 aagcagaata caaggagagc cggcataatt tttaattagc ttttcaatga tctggtattt
    10981 ttccttcatt gtcttggcag ctttattaaa aagctctctg tagcgaacgc aggattgttg
    11041 aatttcctcg gaatatttat tggaaaaacg aatcacccta gttgcatagt catagggggt
    11101 aatgtcttca cctgcatttt ttgctaaaat ttctaattct tcttggtaag tgcaaaactc
    11161 gattatcaaa tcagtgctaa aaaaagcttt ataaataata tctccagaaa gtaggtagag
    11221 gttaggcgtt gtttgttgaa aaacattttt taggcctttt aatccatcgt gaatgtctgt
    11281 gagataaatt attttcattt atattccgta ttcgttaata aaagaatcgg tatcttgtat
    11341 cggctcaaca actacaatat caattccaaa catatccata agtgatttta tgatgatatt
    11401 ggatcgaact tgaaataaaa ttttatgatt atgagcggat aattttttta tctcaatatt
    11461 atgagctgga ttcactttat ttaagaagta atttagattt acacgaattt cattgtttaa
    11521 acgagtcaaa cttaaaaaat ttggaaagag ttttgcaata gtttgaatga tatatctctt
    11581 atgaaagaaa tcgaaactag atataaatcg aactatatca aaccttctcg gtcgactatc
    11641 ccaaatttga aaattactga ttcgaaatct aacatgattg ttttttaccc aaattggtgt
    11701 gagttctact ttatagttca cactttctat tcggaaaaat tttgcgagtg gatagtatct
    11761 tgtgataaag tttccttcta gatatacttt tcctttattt gaatcaattg taaatgtatc
    11821 taaatgttct gtttcttcca caacttgaat gtaaatgagt tcattaatat aacgtagatt
    11881 gagactgacc ttatagtctt ctttaagctg ctcgtctgct gcattgagta gtatatcgat
    11941 tgatttttca ggttgaaata aatctagaat tggagtagtc ataatttgtt cggtagtctt
    12001 catgacttgt ccaatttttc tagtagctct tctgacgaaa tactctatca tactaatttc
    12061 atgttttcga aaaagaattg gaaagcaatc gtaaatttaa aactgctcca gaaattgttg
    12121 acactatagt atgatactcc taacttgtga acgtgaaaaa aattatacac tccctatcga
    12181 tctttttaat agtattggca atcctttcct gtggaaagga aagaaaaaaa caactaatga
    12241 cagattttag ttcagttcag gaaacaatgc agtttccgaa agattttctt ttcggaacat
    12301 cgactgcagc cgaacaaatg gaagaaactc ccaatagtga ctggggaaaa ttcatacaag
    12361 atgcttatag caataaacgt tttgagacaa tgggtcatgg aattgcaaaa ccagggcaca
    12421 tcaataatct aggaagttat tctactgatc atattctaaa aaagaccaat cataacgaac
    12481 tctatgaaaa agattttcaa atggcaaaac agatgaagca taacgcgtat cgtttttccg
    12541 ttgcatggga tagaatattt cctcgtccaa atatgaaaga gccagatagg gaagggattt
    12601 tatattataa aaagctgatt tcttctatga aggcaaatgg tctttatccg ctactaacat
    12661 tatttcactt ctctagtcca gcatggtttt gggaagaaaa gaatggtaaa gttggttggg
    12721 aaagagacga tgcattagag caatatgaaa aatttttaag ggtagttgtg gataattttg
    12781 cagaagatgt agaccactgg tgcacattaa atgaaccgat ggtttttatt ttaggtggat
    12841 atatgcaagg agtcttccct ccgctccaaa ctagaaagga gataacagat attatccccg
    12901 taatgtctag gcttttacat gcccatgcac tttcacgtaa aattattaaa gaatcagaaa
    12961 agcgaacagg caagaaaaac ttaattggtt atacaatgca tacaagagct ttcgaaccat
    13021 ttagaaatta cgctcccttg gatcgtatta tttctgaaaa ggtagagcaa gcttttattt
    13081 gggattttat agatgctgca accacaggag ttttaaaagt aacaaataca gattataaag
    13141 aggaaattcc aggattaaaa gacagtctag attatctagg aattaactac tatggacgtt
    13201 attatataaa atcgaatatt ttctctccaa cgaaatttga gactctgttt tctgatccgg
    13261 atactaaaag agaaattgta aatgatttgg attgggaagc atatccatta ggattctctt
    13321 atatactttc aactgcctat agtaaatata aacttcctct ttatatctta gaaagtggaa
    13381 ctgccgatcg ggaagacaat gatataaaaa gacaggcaaa cattgtgact cacctggctg
    13441 aaatatggaa tttaattcat actaagaaag cagacgttag gggatatatt cactggtcac
    13501 tcattgataa ctttgaatgg gcagaaggat ttgaagcaaa atttgggttg gtaaaagttg
    13561 aatatcacaa taattacaaa cgaacaccta gagcaagtgc tgctgtctat acagaaatta
    13621 tcgaaaaagg catttcaaaa gaactcttag aaaaatacag caaggattat aaataaaacc
    13681 attccataca gaatagattc aaaatcctaa tctgaatgaa tagctcacag tatatttata
    13741 cagtgagcta tttttgtagg gtagaggttc taaatagaat gaatagaccc acattactaa
    13801 aagagcaagc gatgctgtaa aaattcaaga gtttcagaac cattcaaaat tataatagcg
    13861 agtaagaatt aaatctgtta tttttctatc atatcaaatc tatttcaaag gagtaagata
    13921 atcacgaatt cggaagggaa tgtataaaat aaagcagata atgatcatta tcccctcaag
    13981 agaaattagg tagtagggat aaggtcctaa atgatttaaa aaggtattat tgtaagtggg
    14041 ttttgaaata aacatatagt ttgcatcaat catgctattt ataaaaaata caaagacacc
    14101 aaatccatta ataaaaaaga aaacttttag aatcgatttt aaattaggac gaaattgata
    14161 gatccaaatc atatagaagt tagcaaataa gattgctgcg tgtgacaaga aaaaatgata
    14221 gaatcgaaaa tgaggaaatc caagccagag ctctggagat aaaatggcaa caagtgctcc
    14281 accgactcct gtaaaatata caatttcaaa aatagaaaaa cttttgcgga taagtaagat
    14341 tatgcacagg aaaatcgaga aatcacaaag ttgtagcgga agcccgtgtt ctattttcca
    14401 ttcatttgtt gccatatacc aatattgtaa aagtatttct ccacttgcta agatagaaac
    14461 aattaagtat cgaagtaaaa tttcaatcct ttgattttct tggattcttt tttgaaatag
    14521 ataaagaagg attatactta ggaatgtgag gctaatcgcg attatgtgcg atagggaata
    14581 catttcaaaa cgtggttcaa gtagataaaa gttaaataaa ttcatcgata aataaaaaag
    14641 aaggaataga tcaaattaaa tatttttaaa aaatcattct tccatttcat cccatttttt
    14701 aaagtatagt ttgtcatttg attttttatc tctttatcct tagattctaa gatagatcag
    14761 tcctttttag aaagcattca gaatgaaagc ttaggcaaat aaaaaaatct tgtaaataca
    14821 attgaaattc aaaattctgt aattagaatg tctactgata ctccacagtc ccccgatcta
    14881 agaacagtcc gactcaattc cgctgcggac attccaaagg atttcaaagt tcataccgat
    14941 ataaatcggt ggaatccact caagcatatt cctattttaa attggtatat catggcggag
    15001 atcattggtc cgtttagtgt agctcttgct ttttttacga tcatctatat gagtatggct
    15061 ctgcaaaaaa tggtagggct ttttgtaggc aagggtgtag atatctttcg attgattgat
    15121 tattttggat atcttttagg aaatacatta ccttctacga ttccaatggc atgtcttatg
    15181 agtggaatca tggcatcagg tcgcctatcg ggtgattcag aaattactgc aatgcgttct
    15241 gcgggcgtga gcttttctaa aatctatgct aactttctaa tctttggttt tatcacagct
    15301 ctctttgtag cttatttgaa tttttatttg gctcctgaaa atactagaaa gatgaatgaa
    15361 tttaacaatt ggattgccgc ttataatcca atgcttgctg ttactcctgg gcaattcagt
    15421 ggagaccaaa cccaagaact ctttcagaag aaaggaagaa ccctttatac agaaggactg
    15481 aatcgggaaa caggtgagct ttctggaatt cagattcgag agtgggaaat ctacatggag
    15541 ggaaatgaat tcattcaaca caataacatt acgattccaa tgggtggttc gcgcgtaacg
    15601 cagataatct cagcaaagaa tggaacgatc attgaaaaga aaaattcgaa gggagaatat
    15661 gaaaaatcag ttcgattgaa agatggattc gtaattgagt ggaatgaaaa gaaagatgga
    15721 tttgtagtga cggactttat gggtggggaa atggattata atatccctac cgaaaaagaa
    15781 aaaagtgtaa tcggttttaa tataaaaccc gaaacctttt cgtttcctct tctaattaaa
    15841 attcgaaata gtatagaatc agaaggactc gaagaaattc cgggacttga aattttaaaa
    15901 gaattaaata tttctattaa aggtgtaaat ggattaaacc aattagtaga aaaaatgaaa
    15961 atggatataa ttctagattc ccaaaaagga aatgtatctt ccgaggaaat gagtaaccga
    16021 tatgcggtat tcacacagct acagggatta ttaaaagaca caaaaaaaac tctcattgct
    16081 tttaatattg aaattcatag aagacttgca atgccagtct cttgccagat atttttcttt
    16141 ttatcatttc cgcttgggct tgttgtgaag cgctcaggta agggaatgag ttttactctt
    16201 gcgattgtat ttttatttat ctataatgct ttttttatat taggaagtgg gatttcttat
    16261 aagtcaaatg ttccagactg gattggaccc tggagtgcaa atattgtaat tggaacaatt
    16321 agtatctata tcatggctac aagaacagat attagtttta gagatagctt tataggaaaa
    16381 gtatactact tcttaaaaaa gaaactcagt cccttctttg aaaagttaaa tcgtattctc
    16441 tctcctttta caaatagagt taaacgcgtg ttagccccta ttggaagccc aattttaaaa
    16501 ggattacaat ggattttttc gaaagtagca aatggttata aattttcttt tgaaaaaata
    16561 aagagcttaa tcgatcgatt taaaaagaga aaggaaaagg aatagtagaa actcatggac
    16621 tataaagcaa tcggaatttt aaaaggaaat acaattactt tagaaactga aattcctatt
    16681 cgcggaaagg taacagttgt tatccagcaa gaaacagaga acgttacgca acaccaaatt
    16741 ctttcttttt taaaagagaa tatttctgaa tgtagaaatc tttacagaat tgaaaaaatt
    16801 ggaatctttg gatctttagc tcgaaatgag cagacaagtg atagcgatat tgatataatc
    16861 ataaaatttt ctgaaaatcc taaaaatgta tttaatagta aaaataaaat aagagaatcg
    16921 attgaagaga aatttaaaaa gaaagttgat cttgccaatg aaaaatatct caagtcacac
    16981 gtgcgtgatc aaattttaaa agaggctatt tatgacattg aatgacaaat ttagcctaga
    17041 aatactttct aaaatttgag aatgaaaaaa gtaatattaa tccttttttg tatttcttta
    17101 tcccttcacg cagaagcatt cactgtcggg gaagttttga aatacaatat tagtctatgg
    17161 ggaataacgg ttggatattc taccatgtct gtttctggta agacactggt caatggagag
    17221 gaatgtttta tatttgaaac aaaagcggaa ggaactccct ttatcaatcg aatctttaaa
    17281 gtagacgatc gaatcaaaag tatttggaat attcctagaa agcttcctgt ctatagtgaa
    17341 aagaaattac acgaaggttt ttataaaaga aatcattcta ttgtctttga tttaaaaaag
    17401 aaaacagcgc actgggaaca aaatcaattt tccggaaata cagacaaagc aggtgttcaa
    17461 aaacaaggag cgaagtgggt tgaaaaagaa ggggattatg ctaatttgcc gtctgaattt
    17521 caagatatac tttctgctat ttattaccaa agagaataca aggcacaagt cgaagtaggg
    17581 aagtcttttt ctatttcctt atttgatgat ttaaaactta ccgaaatgaa gattcaaatt
    17641 ctaagggaag aaaaactaga attaaaaatc aatggggagg aaaaagaaat cgatacgata
    17701 gtcgttcaac cctttatcaa aacaacagga atctttcgtt ctaagggaaa tatttacatt
    17761 tggatttcaa acgattccaa gcgaatgcca gttaagatta ctgctgatat accttttgct
    17821 ggtcatgtca cagttctttt gaaagaagtg atttgaaatt ctttttgaaa tttagataac
    17881 atactttttg tatttcctac ttcaatctta gaaatagact ttaatatcaa agaagaagtt
    17941 atttttctaa ataaaaagtt ttatgagtaa gtaaaaacaa aaaaatcttg aaaaataaag
    18001 cacgtctaaa aatagtgtta gtcgttgaaa ctatggacgt taaacacaat gactatccta
    18061 gcatcaagga tatagatcag aatattttaa aaaatagaat aaatagaaat ataagccaaa
    18121 gaaatcaatg gaaggatatg agttatttct tctttttaaa tttgataata actattttgt
    18181 ttactgtttc tatttttcca gatgaagtag taaggaaaaa tgggactgtg attagcaatg
    18241 tgaaagcaac gatcaaacgt caaacgatac gaatagagta tgagaacggt cgaatagaag
    18301 aaactagtaa gaaagaattt aaagccgtta gagtaagacc tattgtttgg agtagtctat
    18361 taacgtctct tactatagag cagaagaaaa tagaagaagc attggaaaaa gaaagagttg
    18421 cggaagcatc agcggatggt agtgattggg aatttcgccc tgaggaagaa caaatttctc
    18481 cttggaaaaa tgctgcctta ggactcatac ctggatacag cggtctttac ctcactaaaa
    18541 actattggag tgctggaatc tttactgcat tagaaacagt tgcattagca aactttttag
    18601 atgtagccaa tgcccaaaaa acttcaccaa atatagtaga gtttcaccaa gcgcataata
    18661 ttacgggttg gatactaact tcaggcattg gtggatattt cagatctaga gcaacgaata
    18721 gcaattcccc aatgttagct tctgagaatc tatcttatgg attcattcct acttatgtac
    18781 tttataatca tacttacaaa ggttataaag gaggtttgag tggtagagag atatcgccta
    18841 tcgcagtgga aggagagagt aatcgactcc atgctcaaga aatacaatat gcctctgctt
    18901 tattaggagg tattttaatt gttgatggta tattgtctta tttcagtgct agcgcctgga
    18961 acgaaggaat ttacacaggt ccaaaatccc aaaagccaac aactagactt agtcgtgtaa
    19021 gtagatctgt tttttttccg ggttggggac aaatctacgg aggtgaaaca ataaaaggtt
    19081 actcatactt aaccgtaggg ctagctttga ttggaaatat tattttagct gaatcaaatg
    19141 tagcaaatac ttacagaaaa tatcaagatg ctccttttat tttgtatcct atttatgcgg
    19201 tgtatgcatt tcaaaataat ccggtgccag cctatggtgc ttctaatctt cttgcaaaag
    19261 ataaacaaga aggattaatc gaggcggtag aaaagcggaa ccaagcatgg acgtatttcg
    19321 gagtattctg ggcttggaat ttattggatg cagcatttct ttcaggagtt aacaaaatcg
    19381 acaacaagat acggataaca ccgaatatat cctatcgaaa taatcttggt gtaggcaatc
    19441 gattgagttt agagacctat tatactttaa atctaacact ggagttttaa tatgaagaaa
    19501 atcatatcca ttgttttcat tttatttgtt ctaaattgta gaaataatga ggtagcagcg
    19561 gcaaaacaaa atattctgga tccagctaat ccaatctcct attctctatt taaattatca
    19621 tccgatctaa ttggatcatc gtcttcttcg tgttcaagtt gcggtgctgc tgtctcttct
    19681 aatcaaccta atagtctaat tggcttttct gatagaatca tcattccttc cgaatcaaac
    19741 tcatctacca acccgagtgt ttgggtttta agtgatggta taaattttac aaaattcaca
    19801 gtaaagatag gagattgtgt tggtgcattc agttctacta gttcgacagt ctgtgttcct
    19861 agattggtag gctatgagaa cggtatgtat gcagtatatg gtcaaaaagt tacaaatagc
    19921 tcaggtcagc aaacgatagc cgcagaatac tatggaacgg gaagcaattt aaatactatt
    19981 aatctaacaa ctactagtcc aggaggaaat gcaaatatta atagtacctt cctctactca
    20041 gcaggtaaat ttcattttta tggatgcgcc aataatcaat gcgctttgta tactacatca
    20101 gatggctctt cttggattgc ctctgttggt tttagttgtg aacttattta tcttgcagat
    20161 aacggttctc ctacttgcgg tctctctcga tactttaacg gatcaacttg ggtaaatgca
    20221 ggaacaattt ctggaggggc taacagtagt tattctgctt tatatatcga taacatctat
    20281 cgtgcttgga gaaataattc gggaacactc tctattttta attcaacctc tggaacaatt
    20341 actactggaa ttacatttcc tggctcagcc aatagcactg tgagtatgaa tggggtaaca
    20401 aatagtccta gccaattagc caagacaagt gtcggagtat atcttatggt tacatctttt
    20461 ttttctaata atgtatctac gtttatttgg ctacgttcta gcgatggaaa tacttggact
    20521 tcctataatc ctagtattcc gaccgacctt ggaacgttaa gcaacttatt tgggtatgca
    20581 gctcttggaa gtagctttta cacagtggct caatttaaat cgggcacaaa ttttaataat
    20641 tatttgctta agtcaagcga tggattgaat tggacaaggg taactttgcc ataaaaaaga
    20701 aaggcaataa aatgaagatt gttttgcaat gtttagaatc cattttgata actttgtttt
    20761 tgcctaacaa tactgatcct gttttatttt tctttcgcta ataccttttt caaatactga
    20821 ccagtatgtg atttagaaac ctttgcaatt tcttctggag ttcccatggc gactatttct
    20881 ccaccgccat caccaccttc cggacccata tctatgatat aatctgcgca tttgattacg
    20941 tctaggttgt gttcaatcac aatcatagag ttacctttat ctactaacga ctgcaaaaca
    21001 ctcattagct tttttacatc atcaaaatga agtcctgttg ttggctcatc tagaatatat
    21061 aaggtttttc ccgttggacg ttttgataat tcagtcgcga gtttgattcg ttgtgcttct
    21121 ccaccagaga aggttgtagc ggcttgtcct aatttgatat aatcgagtcc tacttctttt
    21181 agtgtctcta attttcgttt taccattgga atattttcaa agaaagtgca gccttcttcg
    21241 attgtcattt ctaggacctc gtagatattt tttcctttga actttacttc tagtgtttct
    21301 ttgttgtatc tttttccttt gcaaacatcg caggtaacat acacatctgg caagaaatgc
    21361 atttctattt ttaagattcc atcgccctga caggtttcgc atcttcctcc agaaacgtta
    21421 aacgaaaaac gaccagggct atagcctcga agctttgcat cctctaagtt tgcaaataat
    21481 tctcgaatcg gagtaaaaag accagtatag gttgcaggat tggaacgagg agttctccca
    21541 atcgccgatt gatctatatt gattactttg tctatgaact gaatgccttc aatttttttg
    21601 tgttttcctg aaatgacttt tgttttgtta attttatgag cgagttcatt gtataaaatt
    21661 tcattgatta acgttgattt gccggagcca gaaactcctg tgattacggt aagcattcca
    21721 agtggtaaat cgactgtaac attttttaaa ttattatgag aggcttctat aattctaagt
    21781 ttatttccat tcccttttct tctatctttc ggcatgggta tggattcttt tccagaaaga
    21841 tattttccag tgacagaatt tttatctttc atgatttctt ttggagtgcc tttcgctaca
    21901 atctctcccc catgcacacc agcacctggt cctagatcta aaatataatc ggattctatc
    21961 atggtttctt catcgtgttc aatgacaaga accgtatttc caagatttct tagatccttt
    22021 aaagtattga tgagttttgt gttatctctt tggtgtaaac cgatagacgg ctcatctagg
    22081 atgtaaagaa ctcccattaa cttggaacca atctgtgttg caagccggat tctttgtgct
    22141 tccccaccgg ataaagttcc agcacttctt tctaaagtta aataacccac ccctacatct
    22201 cgaagaaatg tgagtcgttg attgatttct tttagaatag gggttgcaat gatactttca
    22261 gatcctttga ttggaagttt tgcggtaaag tcgagagctc tctccactga catttctgta
    22321 aacttatcta tcgttaaacc atggagtcta acggctaagc tttctggttt taatcgtttg
    22381 ccatggcagt cagggcaggg gaggtttgtc ataaatgttt caaaccactg gcgcattgaa
    22441 tctgactttg tttctttgta tcttcttttt aaatttggaa ttactccttc gtattctcta
    22501 gaaaattcaa aatgagagtc ttcttttcta aaatcataat ctatcttgat tgatttgtct
    22561 ccgtatagaa tgattttttt gatattttta gggagtttct cccacggagt attgtaatca
    22621 aattttaaac tttttgcgag agaatggact gttgcgatat accaatagcc ttggcttttt
    22681 ccccaaactt ctaaacatcc ttccatgaga gagagtgctg gatcatttac gataagttcc
    22741 tcttcaaatt ctaaaagaga accaagacca tcacaggtct gacaagcacc ttgtggtgag
    22801 ttaaaagaga aaattctagg acttaattct ggaaaggaaa tttcatgatt attaggacaa
    22861 gcaagttttt gagagaaggt atgatctttt ttaccatctt ctatgattaa cgttccttct
    22921 gattgtttga gagcagtttc aattgagtct gcaagtcttg aacctaatcc ttttttcatc
    22981 acaagtcgat cgactacaat ttcaatggtt gacttaaaag ttttttttaa gactatctct
    23041 tcatctaacg tcttaatttc cccattgatg cgaactcgat tgaatccatc ttttttaatt
    23101 ctttctaaaa catctttgtg ttcgcccttt tgttctcgca ctagtggtgc taaaatttga
    23161 attttagttc cttctggaaa ttcttccact tgttctacaa tttgatctac tgacttagaa
    23221 gaaatttctg catcacaaac tggacaatgt ggttttccaa gtcttgcata gagaagtctt
    23281 agatagtcat aaatctctgt aacagttcct acagtggatc ttggatttct atgtgttgtt
    23341 ttttgttcaa tggatatggc tggacttaaa ccttcaatca aatctaaatc tggtttttcc
    23401 atttgaccga ggaattgtct tgcgtagcta gagagagatt caacatatcg cctctgtcct
    23461 tctgcataaa ttgtatcaaa ggcaagagag gattttccag aaccagaaag acctgtaatg
    23521 acaactagtt tgtctctcgg aatatcaaca ttgatgtttt ttaaattgtg ttctcttgca
    23581 ccgcgaattt ttatgaagga atctgccata tatacaaact tttcaaaaaa agatagaagt
    23641 cgatcagaaa tttcatttaa ctagagaggt gttgaaaagg aagcaattaa ataacgccag
    23701 ttcggcgtaa cagaaaaatt aaaagattac caccgatagg gacgattatc accgataaaa
    23761 gatacgataa ttcaaaatta tccatcgttt ctgcatcgtt cagttatcgg tgtcctcggt
    23821 ggtaatcgtt tatttttctt ttgttgagct agctagatat tgatttttct gtattctccg
    23881 ggttttaagt ttccgagtga aatgtttcca atccgaattc tgattaatct aagtgtagga
    23941 aaaccaatgg cagctgtcat ttttcttact tgtctatttt ttccttcgcg gataatcact
    24001 cttatccaag aggttgggat tttctctctt ttacgaatcg gaggatttct ttcccaaaga
    24061 aaatctggag catctgtgag aatggctata gccggtttgt agctttctga ttgagttcta
    24121 ataccttttt taaatttagc taagtcttct tctgttggaa ttttctctac ttgaactaaa
    24181 tactcttttt caatttcttt atctggatct gtcatctttt gaataaagcg tatatcgttt
    24241 gagagaagca ataatccctc actgtcataa tctagtctcc ccactgcttt aggtttttct
    24301 ggcaaacgaa tgtaatccgc tagtgtcttt ttatcatcta atggcttaaa ctgagaaaga
    24361 acttgaaagg gtttgttaaa ggctagataa atcatgataa atatgtgctt taaaataatt
    24421 tccttctgga aatgtatcta gaataggatg atcgatttca ttttttaatc gaattggatt
    24481 ctttaccttc cgccctgatt cagaaatagc ttctttacaa attctttcaa aatcatttgg
    24541 atgaattcga ttcgaacaag aacagagtat aagtgttcct ttatttgcaa gatttggaat
    24601 cgtttctttg attaattttt tataaatttt tctagcagtt tctttgtctt tttcactggc
    24661 tgttaggttt ggtggatcga tgatgattac atcataaatt gtcttttctt tttctaaggt
    24721 ttcttttaaa tttttaaaaa tgtctaattt tattattcta tgattaccga ttttagtttc
    24781 tgattcagtt agaagtttta gatctttttc tttccgtaaa ttccattcct gaaccaaaat
    24841 tgcttttcta cttgcatcga ttgaaataat ttcttttgct tctgctcttt ctaaacagat
    24901 agataataaa ccattagagg aaaatagatt tagtatcttt ttactagcaa aatattcttt
    24961 atgatttaag atatagtctc ttagatttcg tatatcgttg taaatgccag acttttgacc
    25021 gaatggatca atccaataag agatacttcg gtagtttatc ttgacccaat ccgtttttat
    25081 tcctcttaag attctaggaa ctgcttcttt ggtttcaaag cctgtgcgtt ttggaaccga
    25141 ttgcagaatg gtttcaggtt ttaatttaat tcgattgtct tttttaaata gataaaataa
    25201 atatcgacct atataacgag caaaactttc tagagacttt gcatagcaca ttattactaa
    25261 acaatttcca tgcccatcga tggtcactcc agggagacta tcgttttctc catgaatcca
    25321 tcggatacta tctgtttgtg aaagaagtgg aattcgtttc tccatcctct tttgtatttt
    25381 ttgttgtaaa atttctaagg aaaaattttc tccaaaataa ataattctaa ctgcaatgag
    25441 tcctgtcttt gcataaatcc cgacacctaa ttctttattg cctgcaccga ttagtaaaat
    25501 ccagtctcca tcttttaaag atttttcata ccgagtgaat ttgtttctaa aaatccaagg
    25561 atgaccacct aaaactaaca tggctgttga atttgtgagt tgaattggaa ttaaattttt
    25621 caaagcaaat aaagggtttt atgagttaaa gaaaaagttc gattactctt ggtaatctat
    25681 gaaagttatg cgtgcacaaa atctagtata acaaaaattt ttaaagatca ccacaaatat
    25741 cccatcggtg tttatccgtg tttatcagtg gtaatctttt tcttaaatca atgaacggtt
    25801 aagcgttctt tttagcatcc tcgtaagata cttctgttgg tccaacatag atttgtcttg
    25861 gtctaccgat tttcatatca ggagattcaa tcatttcttt ccattgtgca atccaaccag
    25921 gtagtcttcc cattgcaaac ataactgtaa acatgtttgt aggaatacct aacgcacgat
    25981 agatgatacc actgtaaaaa tctacgttcg gatatagttt tctatcaacg aagtattggt
    26041 cttttagagc tgcttcttcc aactcttttg caatgtcaag aagtggatct ttgataccca
    26101 ttttaagaag tacactatcg catgcctttt tgataatctt tgctcttgga tcaaagtttt
    26161 tataaactct atgtccaaaa ccactcaatc ggaaagtaga atttttatct tttgcttttg
    26221 ttacaacttc ttttactgac attccacttt gtttaatttc agtgagcatt tccaatactt
    26281 cttggtttgc tcctccatgt cttggtcccc atagagcgca gataccagaa gaaatagctg
    26341 catagatatt cgcaagacta gaacctacaa gtcttacagt ggatgtggaa caattttgtt
    26401 catggtcagc atgtaaaatt aaaagtaagt tgagtgcttt tacaatctct gcatctattt
    26461 tatattcttc agctggaact gaaaacatca tatttaagaa atttgcgcaa tagtccaaag
    26521 aattcttcgg atgaaccgca ggctgtccaa ttgacttttt ataagaaaaa gcagcgatag
    26581 tcggaaactt agctaaaagt cttcgaatcg aaatttctct atgttcaggg ttctctggat
    26641 cgtatgaatc ttggtaataa gttgaaagtg atgcaatgat acaagacatg attgccattg
    26701 gatgagcatc ttttggaaag ccactgaaga gtcgttttag atcttcatga attaatgtat
    26761 gtttagtaag agattcattc caaacattta gttctgagtg attaggtaat tttccgtaga
    26821 ttaataaata tgctacttcg gtaaaagtag acttttctgc aagttgttcg attggaatcc
    26881 cacgataacg cagaatgcca agttctccat ctagaaaagt aatcgaactg gtgcaagcgc
    26941 ctgtatttaa atatccacta tcaatagtaa catatccagt ttgtgcacgt aattttgtaa
    27001 tatcaattgc tttttctttt tccgaccctt cgattactgg taattcgtaa tctttgtcgc
    27061 ctatcttgag aatagccttt tctgccataa ttaacatcct tttatgaaat ttagaattca
    27121 agtagatata aaaaaggagt ggaagattta gtaaaacaaa atttttagat caatgatttt
    27181 ctgtgataat tgtttgcttt ctgtttttaa aatttaaata tagattttat gtttatccaa
    27241 aatggtatcc gcatttattt aattctatcc ctattaatct ttttcccaaa ttgcaaacag
    27301 gaaaagaaaa ataatcttct gcctttggga atgctatttc tatattcgca gagccaagct
    27361 agatcttcca attgtaatta taatgatttc actacagatg gaagttcgga tccgtatttg
    27421 tcttctcaat ggtattatag taattcagga gaggatttaa atcctagttc tgtctggaat
    27481 tcaaataggg gggaaagagt tcgggtcgct gtagtcgatg atggtttaga tatttaccat
    27541 gaagatttga cttcgaatgt agttccaggt ggaagttata attttggggt tggctattat
    27601 ggtttttcaa cttctctttc tgacccattt agtgttgtag ctactcatgg aacggctgtt
    27661 ggtggagtga ttggggctag agataaaaac ggattgggaa ttgctggtat tgctccaaga
    27721 tcctgtttag ttggatacaa tgttttagta aattcgacag atgcaaatac ctatactgcc
    27781 atgacttata acgcatctga tgtttcggta tccaataata gctggggcgc aaccgatgga
    27841 aatggaaaga cagccgctaa tagttccctt tgggcaagcg gtgtggatct aggcgtaaca
    27901 actggtagaa atggaaaggg aactgtatat ttttgggcag ctggaaacgg agccaatacg
    27961 aataatgcga cagccgctag cggagtttct gcctatccag ctggaagttt agaaactgat
    28021 aattccaatt atgatggaca agcaaattat tatggagtcg ctgcagtctg tgcactaacg
    28081 gatgatggga aaagagtaaa ctactctgaa aaaggagcaa atctatgggt atgtggatat
    28141 gcgcaaaaaa caaattattc tactacccaa gacggaattt ggacaaccga tataactggc
    28201 tatgatggtt ataatcctct tccatcagat tatttagcaa gtggttattc tcaaagtaaa
    28261 caactctcaa attataatta tagtaaaatt ttcaatggaa cttcaggagc cactccaatg
    28321 gcgactgcta cggcagcttt acttgtaaat gcaaatccaa atttgggatg gagagatgtt
    28381 aaattgattt tggcagaatc agctcgaaaa aatgatccaa ctgattccga ttggctcaca
    28441 aatgcaggat ctaagatttc tagttctgga actccctata atataaatca taaatatggg
    28501 tttggggtaa tcgatattaa ccgcgctgta actctagcta aaacttggac gaatgttgga
    28561 actatgctac aatatgcaac ctcaactatc acagcaaatg ctagtttttc caataataca
    28621 acattagctg ctggagcctc aagttctgtt aatattacaa gtagcaatac atccattaca
    28681 aaaattgaat ttattgagat aacagtaact acagcaagtg ctgatgcatc tgatttagaa
    28741 attgcactta cttctccaca aggaacgtca agcactttag cagaggcgca tatttgtagt
    28801 gttggttcga atacctatcc ttctagcaca agtcaggtag gtgcctgtgg aaattattct
    28861 ggatgggttt ttggaacatc taggcattta ggagaaactc ctatcggaac ttggacattg
    28921 agagtagccg ataggcgatc tggaaccgga agttcttctg gatggggaaa tggaggaaca
    28981 cttacaagtt ggaaaataaa attttacggg agagcaaatt aaatgaaggt atttataaat
    29041 aatagatctt tatacttagt tgtgattttt ttaacgagcg ctttatcgat attagccgaa
    29101 ccaaaggctt taacatggaa agagggagaa agagaacatt taatctatct aagtcaagat
    29161 tatatagcag agatagatcc tccaaaatct gagaataaag agaggaatat acggattatc
    29221 cccatctcgg atacgaaact taaatcagct cttgcaaaag gaaacctacc ttcaaattat
    29281 cagggaactc attcagaagt atttgaggat gggaatagtc ggagaaagat gacacttcct
    29341 ggtgatatat ttgtagaatt cgaggaatcc tggacagaaa agaatgtgaa agattgggca
    29401 gatacagaaa atttaatact tatttcaaaa ttccctggaa tcaaaaacat ttaccgtttt
    29461 aagacagacc caggaattaa aagcctgaat ctagcaaata ctattttgac aaaagctggc
    29521 gtgaaagccg cttatccgaa ttggtggaga gagtttgtaa ggaggtagaa gatttttatt
    29581 cttctacttt gattcctaat tttttcttta attcaatatt ttctttgatc agatctttga
    29641 tttcttgttc tgagtattta aagattctat catgggctct tagaatttta tccatattca
    29701 tttcttcaat tctagaatat tgtaatgctt tttcggtagc ttcttgaatt tcaatcgctt
    29761 cttttaattc tttttctcga agtaaatcta atttttcatt tgctttaata gtctccacaa
    29821 gtgtcaacat ttccgcttca cgcatttcat ttacacgcat gattgcttgg cttacttcat
    29881 tattgtagga tttaattttg gattcatact ctaacatttt ttcgaaggca tgtataatat
    29941 cttgtgaatt tttatattct gcatgcgcca attcaaatag cccttcgaag aatccaacgt
    30001 tttgtaaaca gccagatcca atttctactg cagatgtttt atagaattct gcctgaattg
    30061 ttttttccat aatgagtttt aattcttttt ttcggatagg actctctagt aattctaaat
    30121 ttcctttgtt cgctgtattg atttgaattg gggtattgcc tacttgggat agaagtaaca
    30181 aggagatctc agatctactt ccaaacattt taataaattc atattcaatt ggggaccatt
    30241 cttcctgttt tgcactaata aaaaagagat taatttcctg tggattcaat tctatacttt
    30301 taaattcctt tagaggcaca ttattaatca agatggcaag ttttggatgc ttccataatt
    30361 ctacaggaaa ttctacacca tttgagatat tccagataat tgactttttc atatatctta
    30421 tccttttttg tataaatgtt tttgagacct tgaaaattaa aaatctctag cttgagattc
    30481 atgattgcag ttctagaaat tttttaaagc ataatttatt gtgtttcagt ctagatagaa
    30541 tcagtagaat ctttttgttt gatcaaaact agtaaaaatg acgaaatctt tctatgcaaa
    30601 ctatttactt ttaatcctct aaatctttta tagaataata aggataaaaa gatcttaatt
    30661 cttatggtaa caattttatt acttacagtc tcaaatatat ttatgacatt tgcttggtat
    30721 ggacatctaa aatttaagca tttagattta tggaaagtga tattagcaag ctggggaatt
    30781 gctttttttg aatactgcct gatggttcca gcgaatcgtg taggtcatgg tagatttaat
    30841 gcaactgaat tgaaaacaat tcaagaggca attactctca ttgtatttag tatattttct
    30901 attttgtatc taaaagaaga gtttaaatgg aattatgctg ttggtttttt actcattgtc
    30961 cttgcagtat atataatttt taaaaaatgg taacataaaa tgatagaaac ttttaattat
    31021 cctctcttta ctattcgctc tctttttaat gattttagat ggactgaatc tgaaagatta
    31081 aaatatatta ctacatcttc ttccgaagta aaaaataatt ctctcttcgt ccctctcaga
    31141 gggaatagag acggacacga atttatacca gatgccttaa aaaaaggtgc catttcattt
    31201 ttatgtgaaa aagaccatcc cattctaaaa gatttaaatt tcgatgaaat tcaaaaggcg
    31261 atcattgtgg ataacacact gtatgcgcta ggtaaattag ctacttttca tagaaataga
    31321 tttcaacctt ttattattgg cattacaggt tctagcggaa aaacaactac caaagaatta
    31381 atgggattgg tatctgaaag cataggaaaa tccaatgtag tgataactga aaaaaattat
    31441 aataatgaaa taggagttcc ttttacatta tttagaatta acgaaaaaac aaaaattgtg
    31501 gtttgtgaga tgggaatgaa tcacctaaaa gaaatctcaa gactgactgc aatggtaaaa
    31561 cctagcattg gtatgataac taacgttggt ccatgtcata ttgaaaactt aggctcctta
    31621 gaaaacatag caagggcaaa ggcggaacta attgaaggta tgccgcctaa atctatttta
    31681 tatgttccag aatctatttt acatagaaat atatttcaag agaaagcaaa agaatacaaa
    31741 gtgattctaa aatctttttc tctgaataaa aatccgaatt tagaaatttt agaaagaaag
    31801 acggatggtt ttttattaaa tctatttggg acttctgttc attggaaaat acctggtgat
    31861 aagattttag agaatctatc tggagttttg gcaattgcaa aggaaattca ttttagttca
    31921 gaacaaataa aaaaacaact atctttattt aaagcaaagg ataaaagatt tgtagtagag
    31981 aaaggctatt ttcatttaat caatgataca tataatgcaa atccagattc aatggaatca
    32041 tctctttctt ctctaaagca aatatcagaa ggaaagaatt attttgcaat tttaggtgat
    32101 atgaaagaat taggaaaatt ctctaaaaaa tatcatgaac aagtcgggtt attctgtaga
    32161 aaattaggat tatctgggct tataacatat ggagccgatg caaagtggat tcaaaaagaa
    32221 ttctcttcca ataaaaacaa catttcaaac tcccattttg attatgagtc tatcgatgcg
    32281 ttagtcgaat atgcaaagaa aaatattccg aagggaagct atgtgcttgt gaaaggatca
    32341 agatcaatga aaatggaaag aatagtagag ggattaaaat aaatgaaaaa gaaaaattct
    32401 ccgatagttg gtgttattat gggatctttt tctgattatg aaacaatgaa agaagcttgt
    32461 aatatattag aaagatttca tatttcctat gaaaagaaaa tagtttcagc tcatagatct
    32521 cctgatgtta tgtttgatta tgccaagaca gctgtcaaaa gaggaattaa agtaattata
    32581 gccggagcag gtggctctgc tcatcttcca ggaatggttg catctctcac cactcttcca
    32641 gtgttaggag ttcccattca gtccaaggcg ttaagtggtc tagatagtct ttattctatt
    32701 gtccaaatgc caggaggaat tcctgtagga actcttgcga taggaaatgc aggtgcgatc
    32761 aattcagcat tactagcagt
//
LOCUS       DAOQOS010000058        32110 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_614_length_32110_cov_6.651349, whole genome shotgun sequence.
ACCESSION   DAOQOS010000058 DAOQOS010000000
VERSION     DAOQOS010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 32110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32110
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_614_length_32110_cov_6.651349"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1273
                     /locus_tag="PLF66_13715"
     CDS             <1..1273
                     /locus_tag="PLF66_13715"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HMZ65149.1"
                     /translation="LAHSLIRSGKQELSTTIDLEIQNYARETLYKQLMNLKNKNVKDG
                     SVIVLENATGNILAYVGGAGKDASSAYEIDGILSRRQAGSTLKPFVYALALEKNLIRD
                     TTIINDNPIDIQVGTGIYSPSNYQGDFHGDVTARVALASSLNVPAVKVLSMMHLDDFT
                     NTLESLGFTELREANFYGLSIALGSIEINLKELTNAYRTLANDGFYSDTRVLTDDPFG
                     AKVRVFSEETADTIKDILSDKEARALIFGLENPLVTRFKSSVKTGTSKDMRDNWCVGF
                     NSRYTVGVWVGNFSGEPMWNVSGVTGAAPVWRDILNYLSSGEINISQVEAKKKDTVFI
                     PTKNPSALIKIIHPVNQSIFAIDPDIPDENQALYFEANKIDKEVFWMLNGKRLSEIKG
                     MYFWKPISGKYKLAIVTKANKILDQIEFTVR"
     gene            1270..2151
                     /locus_tag="PLF66_13720"
     CDS             1270..2151
                     /locus_tag="PLF66_13720"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HMZ65150.1"
                     /translation="MNLNFYRPTPVEIFDTINGFDLFIKREDKTFGGFGVKWKKVEGL
                     IQFLKNGHVKEVLIYGNPHSNYVSTFSFFLKKSGFFVYSIFYTNDSKLVTPNSILSHR
                     FSDSVISIKAKANRDFYIQNFKSGFPNGFVIPEFGIHESGIKSLNTLWRDAHFEKMNY
                     LFLDIGTGYTLLSALEYFKEKPIQIIGVAIGNRFDKIQYDLEKNAEKLSVDKNLLKNI
                     QILEPKISPSFASKNKEIENWIREIWEKKQIPLEPIYSGKTLYTVLDFIQKKRFQGNG
                     IYVHQGGLLNHLKYFMP"
     gene            2343..2960
                     /locus_tag="PLF66_13725"
     CDS             2343..2960
                     /locus_tag="PLF66_13725"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HMZ65151.1"
                     /translation="MSKFKICIADDHPVVREGLRVILQADPRFKVVGLFPDGKSLLDF
                     LKLNSCDLLILDIGIPGGEDFHILKQVKSNTPEMKVVIFSMYEGAQFFLEAKKHGADS
                     YVLKKDSYSLMPSIVEMTLNGVSYCSEELKRYKDSSDVKVTLNTIEEKIVKGLEQNLS
                     YKEIAESIQKSEKTVQSYIYKMRKRFQVQNNTELLMFLYKNYSHP"
     gene            3037..5046
                     /locus_tag="PLF66_13730"
     CDS             3037..5046
                     /locus_tag="PLF66_13730"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HMZ65152.1"
                     /translation="MPFTMYLHRLNFILLFLFLGIPNLLNAQTFSVEKKMNGILIENY
                     YFLADPNAKLNIKDVILSESFRPIKEKKSLGIEPGAIWLRIDFENSSDRPIEWILQVV
                     YHQFDFVDFYSIHDLEIKKELHIGDHRLFEERLIPHGSNLYPAKISPKEKCRIFIRLA
                     YEKEGRFNTALKIWTPKEFQNNSNFYHIFFAGSMIGVFTLVFINLFFYISTRTKAYIW
                     YILYQLSIILMGCSFTGIGYYVIWKDWIWFTDLIPTLSPALVFMNAATFTISFLETKK
                     FSSKIDLFLKLLAVLHILPICGAILGYKAFASDLVMFLSSFLVLYPFLGYYFWYKGKR
                     EARFYALAWTLWTTGMLTGLFRIAGKIESTFIFEVIPFLGVILEALFFSLALADRINI
                     FQFEKEVAEKKYTQSLEKFNEDLSLKVEEKTKELQLKNQILASMNKDKDTFLSLIAHD
                     LLGPVDSFKMATQMIQSNRKKVKLKDKKNFQKLLQSSAENIYSLLENLLEWAKSQKGI
                     IVFSPKELKCREIAEKTIQLLRPNAQAKQIIIENNIKINLSLFADENLLTTIFRNLIS
                     NAIKFSIEGGKVIVGISKNSPKEITFFVQDNGVGVEKEKLKSLFEPGVNASERGTKGE
                     SGVGLGLQICKEFTELHGGRIWVESKLGKGSIFFFTIPKRTTSSG"
     gene            complement(5030..6172)
                     /locus_tag="PLF66_13735"
     CDS             complement(5030..6172)
                     /locus_tag="PLF66_13735"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HMZ65153.1"
                     /translation="MYKNLFIIFICQALVQSGSILMTSTNTMIGLEITGSQTLSTMPV
                     AFNTIATLLLTIPASFYMARFGRKSGFILGILFALVGISLVILGISQKNFIIYCLGTI
                     FIGFQSSFSTYFRFAAAEVVHRDFKSRAVSLVLASGVIAALIGPNLYKWGLSVWKDLH
                     FIGGFILSYPLYFIAFSVILFLNTGKQATLDWKKSKSIKDLFLHTPLPKIIGIGSIAY
                     VVMVLIMTATPLGMKHHHFEMNDITQIIQFHILGMFVPSFFTGSLIKRFGDRKIMLIG
                     TFLFLICIGINFIDHTFLHFAYSLILLGIGWNFLYVGATTLLSYQIHPEDQPKAQAIN
                     DFVVTGFSAISIATSGSLHELLGWVNVNLIAIPITILGIIIIIRKK"
     gene            complement(6193..7233)
                     /locus_tag="PLF66_13740"
     CDS             complement(6193..7233)
                     /locus_tag="PLF66_13740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016758184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HMZ65154.1"
                     /translation="MAIIDEIKRELSKVNHPEQKRDLVSLGMLGDINPTDDGFLIKIK
                     TPTADRKLQIGLEAQIRQSVSKISNIGKIKIKFDVDPNLKFDDGNRIPGVKRIIAIGS
                     GKGGVGKSTVTANLAMLIASKGYKVGLLDADIYGPSIGKLFGINGRVALGTDENRIEP
                     VQKYGIKIISFSFLVDPNQPVVWRGPMLGKALEQFLYDVIWGELDYLVIDLPPGTGDV
                     QLSLAQLIDINGAVIVTTPQAVATLDASKAGSMFNQVKIPILGVIENMSYFIPPDLPD
                     RKYAIFGEGGGQKLADSFKSPLLGKIPMMMEIMQSGENGNPIVHADKNSPVVKAYDEI
                     FTQLEKEILNWE"
     gene            complement(7674..8033)
                     /locus_tag="PLF66_13745"
     CDS             complement(7674..8033)
                     /locus_tag="PLF66_13745"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HMZ65155.1"
                     /translation="MILVDTNVWVEFFRGSKSSIALSDSIESGDVYLHPFVLTELILG
                     GLKKDIQIRMENFVNVSILSEIQLRKFIAEEKLFGRGLGFVDIHLLASARIQNCNLWT
                     FDSYLKKAADYLGIDTI"
     gene            complement(8033..8221)
                     /locus_tag="PLF66_13750"
     CDS             complement(8033..8221)
                     /locus_tag="PLF66_13750"
                     /inference="COORDINATES: protein motif:HMM:NF021474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HMZ65156.1"
                     /translation="MNTTLNLDKDLLNQALKLYPDKTKKAIVEMGLRELILASERREL
                     AKLFGSQKNLISPRRVRK"
     gene            complement(8444..8839)
                     /locus_tag="PLF66_13755"
     CDS             complement(8444..8839)
                     /locus_tag="PLF66_13755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65157.1"
                     /translation="MKNKMNLERKKKIIDNMQIYIAELKGKISFVNESNTSFKEISYS
                     TGSFFSDVDTVIQSAIPRFRLRDIPAKSYVIIEDVDVYEDGRIIYSNAKFVETNGEIT
                     EVRYLKKIEILPSDPFPGKLVERELEEIS"
     gene            complement(8921..9661)
                     /locus_tag="PLF66_13760"
     CDS             complement(8921..9661)
                     /locus_tag="PLF66_13760"
                     /inference="COORDINATES: protein motif:HMM:NF016041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HMZ65158.1"
                     /translation="MKDWVWNWTFWELIFYSSILNLLIFVACIYTGRIFQKFFQKNKA
                     YELMAENFSKIEIVFSISTVLINTIILLLGWVLWKNHFIKIKTDDSMPIIVFDALVLF
                     LSMDVCMYFFHRIAHNPFIFNLVHTTHHEFKNPKPVTLFVLNPFETIGFGVLWLILLC
                     LYSSSWYGMIFYLNLNTLFGLIGHLGVEPFPSNWVRIPILSKITTGTFHILHHQNLDY
                     NFGFYTTIWDELFNTTHPEYKSRFEEIA"
     gene            9821..10372
                     /locus_tag="PLF66_13765"
     CDS             9821..10372
                     /locus_tag="PLF66_13765"
                     /inference="COORDINATES: protein motif:HMM:NF021886.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HMZ65159.1"
                     /translation="MLKYAILGYLNYRPMFGYELKQAMDGSTSFFWHSKLSQIYMTLK
                     SLEKEKKLKSEIENQEDKPDRKRYTITKLGKEDLNTWLETPLTDIPQNKDELLLKLFF
                     SANQDKKSILSELRIQKKLHENQLNTYINVTAKVIKDAKKENPACAKDSILWEATRRM
                     GELYEKAYIQWIDETIRVVESKL"
     gene            10382..10813
                     /locus_tag="PLF66_13770"
     CDS             10382..10813
                     /locus_tag="PLF66_13770"
                     /inference="COORDINATES: protein motif:HMM:NF021435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2141 domain-containing protein"
                     /protein_id="HMZ65160.1"
                     /translation="MRLKKIFLIGILFFAFPSFTETNFSKLMIRIDGVKNKKGTVNIA
                     IYKSDADFKNENFAYVSKISSDASVYFVFEKVEYGKYAIAIYHDENENRKLDRNMIGI
                     PSEGYGFSNNAKGSILGPPKFQDTSFSVEEPQKILTIQMSY"
     gene            10828..11991
                     /locus_tag="PLF66_13775"
     CDS             10828..11991
                     /locus_tag="PLF66_13775"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HMZ65161.1"
                     /translation="MKYTTVLIFLFFMQCSIASIKEGEENIVKTFQKTIAKSKFSKAA
                     TLLVHSDTLKVHIESSAGFLDNSKTIPVKKDQPFHVASIGKIFTTVLIYQIIESGKLK
                     LDDRISKVLSPDLLKNLFVYEGVDYSEKVTISDLLEHTSGVADYFEALDKNDKSLLDE
                     IQKNPDKLWTPKEILDYTRLNKKAVGKPSEKFHYSDTGFILLGLIIEKITNKSFEKII
                     QEKIFSPLGMKNSYVYLRSEPIDKSKLAPISTMMLGDKDVTNYKSISADWAGGGIIST
                     AQDLLLFHQALIKGKLISNLNYSSLVGKNKFLDGIYYGKGLMTVRFNDMSFLMPKTPE
                     LYGHSGLLGTLLFYSPEYDSYIIANLGSTEDVGESFEMMFWIMQDLKEIKNLK"
     gene            12001..12840
                     /locus_tag="PLF66_13780"
     CDS             12001..12840
                     /locus_tag="PLF66_13780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65162.1"
                     /translation="MVSLRFFKTSKENFDLKILFYISFICFLISSFGFVFDGRVLNYT
                     PLWLKPLKFAISSLIYSITLIYFVRFIPSERFLVWANKITSYGLILELLIIYFQAARG
                     RMSHFNFLTLEDMVLFQIMAVAIVLVWLAFFIYIKGFFSIPLGRNIFLFSIRMGLIIS
                     FLSMPMAFTMTQPSSADLKKIEANKSPIGFTMGSHSVNEKDQTKRLPFTSWSRTGGDL
                     RIAHFLGLHGMQILPLFGMFLFRFRYDYKMGKIIISLLSIIYFSFMVFTLMQALEGVP
                     FIR"
     gene            12850..14421
                     /locus_tag="PLF66_13785"
     CDS             12850..14421
                     /locus_tag="PLF66_13785"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HMZ65163.1"
                     /translation="MENKYDVIVIGSGIGGLAAATLLSKIANKRVLVLEQHWELGGLT
                     HEFKRKVKYSWDVGLHYIGNMDKNSLPMRVYNFISNGNIKWNSLPYEFERFIYPDMTF
                     TVPSNENEYIQKLSKSFPDETKSIQKYFKDMKKATQWATLNMMATLVPSFFGFFIRLW
                     NKKTESIALSTTKDYLDLNFKNEKLKSLLVSQWGDYGLPPSKSSFLIHSIIATHYLDG
                     AIYPEGGAGSIAKYAEPIIEANGGKCLLSSMVTGLIVDHNTVIGVEVQKKRGEKRKEI
                     YQAETVISCTGALHTYGELMKGHISESYIEELKKLPRGKSAVTLYLGLKNDPREMGFK
                     GENSWIYESYDHDLEAANAANSLLAGNPRACYLSFPSLKNHSAKGFTAEIISFADYND
                     FTQWENSKWKKRGDDYEKLKSKIAEGLLNLVEKKYPGFKDLVDYTELSTPLTYIELAG
                     RSNGEFYGLASTPERFKIKWLGPKTPLKNFYLAGTDAMALGITGAMMGGVAAAACVLD
                     PGGFPKIIKGVNRKV"
     gene            14424..14864
                     /locus_tag="PLF66_13790"
     CDS             14424..14864
                     /locus_tag="PLF66_13790"
                     /inference="COORDINATES: protein motif:HMM:NF019032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1499 domain-containing protein"
                     /protein_id="HMZ65164.1"
                     /translation="MAPEPILTFLLICCSSFIGPRTGVYDGELNYCPPTPNCVSSQSW
                     KLNPIHSIRPYQYENQTKQEAYDQLKKLLDSKENVHVMADPENFYIRTYYFTKVFRFP
                     DKVEFLFEENQPIVQIRSASVFGIFDIFHNRIRMEYIRKELGWL"
     gene            14939..16102
                     /locus_tag="PLF66_13795"
     CDS             14939..16102
                     /locus_tag="PLF66_13795"
                     /inference="COORDINATES: protein motif:HMM:NF013296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HMZ65165.1"
                     /translation="MSSKPPVINTSRKFSDGMASAHSLLIQAKNGIKGNQKGERYAIP
                     AFNVTTFQSINAAVSAAEMLGASAILAFSNSALKSFGLGDQTFGVEVTSEYVAKLAKR
                     SKIKIATHLDHGDYISESGRKVVQAAVQGYTSVMADNSTDHKAERAMPLSDNIDLTRE
                     VVNMAHPLGLSVEGELGVLAGEEDEDTKSDHSTYTNPEELEQFVKGTGVLMLAPTIGT
                     MHGPNKGKPGHKVKLNIELAHQLLKIADSINPEILFVAHGASTLYPEVVDFVLKNLSP
                     SHPSVQKWKDFVGTDWDQIQGLIGAGFAKINTDTENRQTYVAALIGALNKDSAKIDIR
                     HYDKVTTEALTQSYIKKFILAGDYGVWYEPKIDINKFKFDLSIDLATATGTGK"
     gene            16292..17137
                     /gene="dapF"
                     /locus_tag="PLF66_13800"
     CDS             16292..17137
                     /gene="dapF"
                     /locus_tag="PLF66_13800"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017851178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HMZ65166.1"
                     /translation="MQKIKFTKMEGIGNDYVYIDATKENIRLTPDQIQKLSNRNFGIG
                     SDGVIFIRKSDKADFMMDMYNSDGSSSEMCGNGIRCVGKYIFDYKLTSSKTPKIETGK
                     GVLELNMETDAHGKVNMVTVDMGEPILVPEKVPVLLPGTDPIIEHTIDIKGYKIKFTA
                     VSMGNPHAVIFVEDSDQFPVTEIGPILEHHPLFPKRTNVEFVSIRGKDHLYQRTWERG
                     AGETLACGTGACAVHVAANLTNRAGKKTRIDLRGGTLYIEWTESGSIIMKGPATTVFE
                     GEFQI"
     gene            17312..17590
                     /locus_tag="PLF66_13805"
     CDS             17312..17590
                     /locus_tag="PLF66_13805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65167.1"
                     /translation="MYRFFIVSILFSGLFINSILSAKCYSFRKIESIKVCIEGDSNAD
                     RRKAQDVCKDVLGADCGGVAGYTGSCAKSEKLKCYNKDAKEQKHITVD"
     gene            complement(17610..18251)
                     /locus_tag="PLF66_13810"
     CDS             complement(17610..18251)
                     /locus_tag="PLF66_13810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012387959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlbB"
                     /protein_id="HMZ65168.1"
                     /translation="MGTMADKARILYLLLLILFLLTVGFFIFDYYGLIDADEIFPALA
                     KKPSPVNWDKESPTEVEKLEYKKAREKLDEEIAEIERIRQSLDEEREKLQNENEKLNE
                     MKKSIQEKEKQMAMAKKDLEKREAKVKVLANKIANMPPPKAIALLENWPDQDIIDVFK
                     QMDKDAEEEGTNTITNYLITLFKDPKRQATITNKWLDHEADRIPNDNTSVLTE"
     gene            complement(18261..18446)
                     /locus_tag="PLF66_13815"
     CDS             complement(18261..18446)
                     /locus_tag="PLF66_13815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65169.1"
                     /translation="MPNLSNKSDEGNSFVSLLYNINSEKAPKDIKIQSSLVRLTNKVN
                     ELEAAVFRLDKAIHDYN"
     gene            complement(18418..18984)
                     /locus_tag="PLF66_13820"
     CDS             complement(18418..18984)
                     /locus_tag="PLF66_13820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65170.1"
                     /translation="MKKFEFDLETVLELRKIRVDEELKKLSIIVGNLNRLQNEIKDNN
                     RIILNSTTKYSGSDIKTLRLFEGYIKSLYLQNETLQKSMNEQQEILQEARQRVIEVEK
                     DAEVLEILKRDAWKEFHEKMLKQERSEEEEVTNNEFIHGRKQPDEKTETPTVKIKKKI
                     KTVKSDKSKPKSEYEKLMEYAESLKQKR"
     gene            complement(18987..20351)
                     /locus_tag="PLF66_13825"
     CDS             complement(18987..20351)
                     /locus_tag="PLF66_13825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012387962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030257 - type III protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0046034 - ATP metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FliI/YscN family ATPase"
                     /protein_id="HMZ65171.1"
                     /translation="MIEKKFTEKIDILSKYKLIVSKTEPIRKSGKVLKVLGNVIFSQG
                     PPDSKIGEIMEIERQDGKGYLQCEIIGFDGHKYTLMPLGEVSGIFPKAFVFSSGKKLT
                     IHVGKELLGRVLNGAGRPIDGKGIIVTGEERSPENETPNPLDRPIIREQVVTGVRAID
                     GLLTIGRGQRVGIFSGSGVGKSTLLGMIARYTNADVNVISLVGERGREVNEFLENEIG
                     QEAMAKTVVFIATSDAPKMQQVNCALLATSVAEYFRDQGLHVNLMMDSLTRFAQANRE
                     IAVSYGEPSITRGFSASVFSKLSKLIERSGTSKSGGSITGIYTVLTEPDEMNDPVADA
                     VRGYIDGHIVLSRELAERNHYPAIDIPSSLSRVMPYIVGEDHKMYSDFVRELIATYKK
                     SEKLIMLNAYVKGADPKTDLAISKKIMIDEFLTQKIEQKVNFQDMLYQLRDIFLTPVD
                     DELL"
     gene            complement(20377..21309)
                     /gene="fliH"
                     /locus_tag="PLF66_13830"
     CDS             complement(20377..21309)
                     /gene="fliH"
                     /locus_tag="PLF66_13830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008592071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HMZ65172.1"
                     /translation="MAKLVFKPMQIAETQSGNDALELAIPEKYKKFHKAEEEEEFEVD
                     QEGNIIEQYQGPSIDEIEAELDRYRQETEEQVRQMLADAEAKAEKIVEDGKTRAFQLI
                     QDSKEKVKTEEDSGRAKAEQILDRAKLEVERMIKEAEMKVAEIEHEAYQRGYDAGREV
                     GFKKGQGEVRRLIDRLGTIVGKAIDIREDIIQASEKQMVEMILIIARKVIKDEIIERK
                     EIVLNNIREALKRIKDRDRVDIRVNFSDLEITTAHKDELIKLMESLRKVNIFEDSRID
                     RGGVIIETDVGAIDARISTQLKEIEEAIRNAEPI"
     gene            complement(21313..22281)
                     /locus_tag="PLF66_13835"
     CDS             complement(21313..22281)
                     /locus_tag="PLF66_13835"
                     /inference="COORDINATES: protein motif:HMM:NF013838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliG C-terminal domain-containing protein"
                     /protein_id="HMZ65173.1"
                     /translation="MTRTKNQNSKAAILYNLIGDHLPKEVLAQLSQDELEKLLQKVTA
                     LKAPSANEEKNVLSKFADTFNKYRGVSNSALQAKIAKEIEKLIQETANNKVSAIAELR
                     KKKRSELALIVNEEEPRLIALVMSFANPDEASSLIEDFPEKKREEIIYEIHKIDFHSE
                     TVRNELERFLNFKYELIKNNQTVSKVKNRGSKITAEILSRVSPHVSFSLFSKIKKKNP
                     DFAENINKHFYTIEDLQYANRSSLTEFLSKIHPIVIASSFKGIETSVKDQLIERCEPW
                     LAKQISLEMDSMGPISLAEIEEAQTAIITLLNESIDKGTIKLWKVG"
     gene            complement(22278..23984)
                     /gene="fliF"
                     /locus_tag="PLF66_13840"
     CDS             complement(22278..23984)
                     /gene="fliF"
                     /locus_tag="PLF66_13840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002146932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HMZ65174.1"
                     /translation="MPESLQKVIAQIKELFDKLDSTKKIILGAVFATVVIALIILSTF
                     SLEKNAVILFKDLQQKDYGEITKKLDALGYRYSGSGDSVISVDPEKRQEIITKLAQEN
                     LIPVGVQGWELFDIEKFTETQFDKDVKKYRALKGAIEKSLMTLQSIEKADVNIAFPET
                     EYFSQDGSTTPVKAAVILHFVPGIEGLSRKEIKGIVNLVSRGVPKLKPEDVSVADSTG
                     KIISDFEEDLEKEKIELRLIQEKMRIADAERVKKLVDIRNTLRWLLAGEDRVDVTRFE
                     YDLNWDEESFKENLVSPVIAKADNPNTPYDETELVDGYSLKISDKETSEKFNGRGFTP
                     EGPAGTEPNIPPGYKDVDYQKANYEKDEKIRNYEFNRKVRDVKKQKWKIDKITLAVAI
                     DGIWTKKESEDGYKFDRTYIPVSDEDLRKIKKNLETAIGADKTRGDVVNVMSIPRDRT
                     AQFAAEDAELARQKAIRNAIIISLAILTFLILVILIYRAIKKEIARRRRLREEELAAQ
                     QQMMREAALRVMEEGGADVELSLDEKLRKELLENAINLAKEKPEDVAQLLRTWLSEEE
                     AG"
     gene            24097..25446
                     /locus_tag="PLF66_13845"
     CDS             24097..25446
                     /locus_tag="PLF66_13845"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HMZ65175.1"
                     /translation="MFDVNFILILLFSGAILFAYDRHYNTPLIYVKEERVYIATISFG
                     FAAVGSALGIQIALSKLGFQENILNNAFIYSALVEESVKCVFILLVLSYNRVNEVLYD
                     GLYYGLVFGGAFGLIENIIYASVLPFWPMMLRTITSSALHLLNGGIIGYFTAKYLFTA
                     KRHRSSYLLQGFLLCYVSHALYNLAGFTGGKFLMILPILLVFNFILVEFISAYARSSL
                     PKFALDLIHLSILDYELIRRHTKYESWLYNEQKVFKKKTNLFQKVSLNRILSVSTAFL
                     ISLFFITLYFLFPEIRSKVFKEILFYEYVSIFINYPLMVSISILFAGLVNPDFFQREI
                     LRVPLMCVLSAKSKDYEETTVVFYLTFHGFYAPFMYPEKLEGDLLLSFQIGNKNIENI
                     KGKTIWIHGANNETQNIKSRLSVTGALIQFEKNPFFLITYWNWARWVLRIQNLLRLV"
     gene            25448..26077
                     /locus_tag="PLF66_13850"
     CDS             25448..26077
                     /locus_tag="PLF66_13850"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016544445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HMZ65176.1"
                     /translation="MIEILYRDEFLLAAVKPAGIPVHETKDPKRENFTGLVQKELSLP
                     YIRTVNRLDLETSGIVLFGLTEERNKEIDEILKNADKYYLALVEGNPPDEFRIESFLK
                     DGNKRVSTVRSGGKKAITEFKTLERLNDRGYSLVSAHLITGRRHQIRIHLFENGFPIL
                     GDKVYTNRKTPLANRSMLHAYELHFQNLFGENIQIKAKPPNDFLKFLNR"
     gene            26511..27032
                     /locus_tag="PLF66_13855"
     CDS             26511..27032
                     /locus_tag="PLF66_13855"
                     /inference="COORDINATES: protein motif:HMM:NF019320.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme NO-binding domain-containing protein"
                     /protein_id="HMZ65177.1"
                     /translation="MKGVIVMCLKEMIETNYGSDKWIEVLVNSNVDTKTAFIAFADID
                     DAIVLKMLGSASKILKITSIQAADAFGDYWVNVYAQKMYRSMFVGKKTAKEFLLSMDQ
                     LHVGVTQNIKNAHPPRFEYELKGEKTLIIKYKSDRGLIDIFIGLIKGVGKLYKENLLV
                     SKLGNNLVQVQFN"
     gene            27063..27755
                     /locus_tag="PLF66_13860"
     CDS             27063..27755
                     /locus_tag="PLF66_13860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65178.1"
                     /translation="MNKKILLISFFLYFNCYNENRQLKPLSNKYFLKTIEANVPIELK
                     EIQIFREWTNESYLKPPNLNSYIIYVDSEKFKIYFKEKLQEKIAESNLVQEDNVKVEI
                     ETFSLSYKIVKDFGIIIPHQIITSNMQVRTKVTILNRVETIEYTKKLVSESNLGSPFL
                     IFYSLTALMRMGNVVLGTAALGSSILTGGIFYFDAMSDNIERYVESDLDRYSYYLRNK
                     LEKIKITIKERE"
     gene            27952..28686
                     /locus_tag="PLF66_13865"
     CDS             27952..28686
                     /locus_tag="PLF66_13865"
                     /inference="COORDINATES: protein motif:HMM:NF013838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliG C-terminal domain-containing protein"
                     /protein_id="HMZ65179.1"
                     /translation="MSFDDRIFSKFDFSEVDREKCIPLIEEIIRLAELSRKQGLLLLE
                     EELSTIKDPYLKMGLQLIIDGTEPDLVEEILDRIVFFSNASPERVLEFCIMRTGILSI
                     QAGNNPYVLNHQLYAYIGIEYIEEYNFTEPETRIRELVVNSNFVPLDISLEFNFLFSR
                     LDDFSVQRIVREIDLNVLGCAMTNVSIECESKILSNISTRAALLLSQERQFRTIPLEE
                     CIENQQMILELYERLVEMGEIIRVEE"
     gene            complement(28687..29979)
                     /locus_tag="PLF66_13870"
     CDS             complement(28687..29979)
                     /locus_tag="PLF66_13870"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HMZ65180.1"
                     /translation="MKQVIFILLFLFNTLSSEEIKSIRYLKPNGSLSEVSDLVIENGF
                     ISRITESKPKDTIRYILPSFCDANVTLGVNPAGGDQDRKSLETSLKAFLAFGFTHIQS
                     IADGPWIQKVKSDIDSGKLVGPKITIAQRPLLAKSKETKDISNQLYFVTENKEALQTE
                     LNAQLNAFNKTIHIFNRHNEDSSLLFDSELLLDLRLSSEAKNKRITIHTFGDRISILD
                     SLISGNRYLAHPIPNDMNREIARQHIQEMHLMPMLNVYQNLHLNADSDGIDELNYLKK
                     KSKFFTDYFAASYESALKLELEEIEIQTRKKEFKSYIDFLKTHPILKEKMILGSGSGN
                     RLSFAGVSGMQELRILAKAFDTNEIFMIPTKNTCEYLGGSYRGLIQVGEEANIILVKE
                     NPTKNLETLFQIEKVYLNGKAVTLYLPEPVKKKPFRKK"
     gene            complement(29976..31232)
                     /locus_tag="PLF66_13875"
     CDS             complement(29976..31232)
                     /locus_tag="PLF66_13875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65181.1"
                     /translation="MDQLLLKLHQAIQFLGKISILFLFTLLFSFCASGDELEIKPNEA
                     DKSYWDEVASYLAGVPLDENNRFLPLTSLNYYKNYSRSIDKAWGKITSNYINHVTEWS
                     KEFIPDSHKENTSLYLLSGADFINLYHFYPKSNRYIMVGLETPGYINDPILFKPEELK
                     NAFYTLDASLNQISTQNYFTTILMEKKFANKYFPGVAPVLLVFLKRIGFQIDELQRIS
                     ITPDGIIKEIKSEEVKDVPDAYQGVRIIFHSKDNPRFRELIFLKFFVSPNSMEPTSVE
                     GKFFSKQKKLNLIMKSAIYLMHMEKYKNFIQSILSKTEIVSEDDSGIPVRYFQKEDWN
                     IDVFGSYTKRLSIRYVPKEANMFKSEKGNIRTESQKFTQEDLKEIYETSAKPLRFKYG
                     YGGAFRGTARNNLILLRRRALLKTKA"
     gene            complement(31368..>32110)
                     /locus_tag="PLF66_13880"
     CDS             complement(31368..>32110)
                     /locus_tag="PLF66_13880"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HMZ65182.1"
                     /translation="IYALVYGKSTYPSELLNVKDETKTKTILWLFYLIQVDRRIILVD
                     TGFTEKKYVSSFQISDFKHPTLLIRNLNLTPEQVTDIILTHSHFDHIGGAHYFPNAKI
                     YINTKELEVFQSSDGFKQFEKVFEVRNFRNQIQKIDSNFYLTPEISIYFSGGHTIGSQ
                     YVHLKTENKEFVITGDECYLAKECLEGIGLSAKAAYNLSNNKIFIDKLSELRKKNTRL
                     EVLTLHDFELVRTTGIPKNGIITIYEKK"
BASE COUNT        10225 a         5169 c         5539 g        11177 t
ORIGIN      
        1 tttagctcat tctttaattc gaagcggcaa acaagagtta tctacaacaa ttgatctaga
       61 gatacaaaac tatgctaggg agactctcta taaacaatta atgaatctaa aaaacaaaaa
      121 tgtgaaagat ggatctgtta tagttctaga aaacgcaact ggaaatattt tagcctatgt
      181 aggtggagcc ggaaaggatg cttcttctgc ctatgaaatt gatgggatat tatcgagacg
      241 acaagcagga tctacactca aaccgtttgt atatgcgctt gctcttgaaa agaatctaat
      301 tagagatact actattatta atgataatcc aattgatatt caagttggaa ctggaatata
      361 cagtccttcc aattatcaag gagattttca tggagatgtt actgctagag ttgcactcgc
      421 ctcttcgctt aacgtccctg cagtgaaagt tttaagtatg atgcacttag atgattttac
      481 aaatactctt gaaagtttag gttttactga attgagagaa gcaaatttct atggattatc
      541 gattgccctc ggttctatcg aaattaatct gaaagaatta accaatgcct atagaactct
      601 tgcaaatgat ggcttttatt ctgatacaag agttttaacc gatgatccct ttggtgccaa
      661 ggtcagagtt ttttctgagg aaacagctga tacgattaag gatattttgt ctgataaaga
      721 ggccagggca ttgatctttg gtttagaaaa tcccttagtt acacgtttta aaagcagtgt
      781 aaaaacagga acgagtaaag atatgagaga caattggtgt gttggattta atagtcgtta
      841 tacggtaggg gtttgggttg gaaatttttc gggtgaacca atgtggaatg tgagtggagt
      901 cacaggggct gctcccgttt ggagagatat tttgaattat ctttcatcag gcgaaataaa
      961 tatttctcaa gtagaagcaa agaagaaaga cactgttttt attcctacaa agaatccaag
     1021 cgctttaata aagatcattc atcctgtgaa tcagtctatt tttgcaatag atccagatat
     1081 tccggatgaa aaccaagcac tttattttga agcaaataaa atcgataagg aagtattctg
     1141 gatgttgaat ggcaaacgtc tctcagaaat taaaggaatg tatttttgga aaccaatttc
     1201 tggaaaatat aaattagcga ttgttacaaa agcaaataaa atattagatc aaattgagtt
     1261 tactgtaaga tgaatttaaa cttttatcga ccaacacccg tagaaatttt tgatacaatc
     1321 aatggctttg atttatttat taaaagggag gataaaacat tcggtgggtt tggagtgaaa
     1381 tggaaaaagg tagaaggtct gattcaattt ttaaaaaatg gtcatgtgaa agaagtattg
     1441 atctatggaa accctcattc aaattatgtt tctacctttt ccttcttttt aaaaaaatct
     1501 ggcttttttg tgtattctat tttctatacg aacgactcta aacttgtaac tcctaattcc
     1561 attttgagtc atcgattttc tgattcagtc atttctatca aagcaaaagc aaatcgagat
     1621 ttttatatac aaaattttaa gtcaggattt ccaaatggat ttgtgattcc tgaatttgga
     1681 attcatgaat ctggtattaa gtctttaaat actctttggc gcgatgccca tttcgaaaaa
     1741 atgaattacc tttttttgga tataggaacc ggctatacat tgttatccgc tttagaatat
     1801 tttaaagaaa agccaattca aattattggg gtagctatag gcaatcgttt tgataaaatt
     1861 caatatgatc tagaaaaaaa tgctgagaag ctttctgttg ataaaaatct tttgaaaaac
     1921 attcaaattt tagaaccgaa aatttctccc tcttttgcca gtaaaaataa agaaatagaa
     1981 aattggattc gagagatttg ggaaaaaaaa caaattcctc ttgaaccaat ttattctggc
     2041 aaaactcttt acactgttct cgattttatt caaaaaaaga ggttccaagg aaatggaatt
     2101 tatgttcacc agggtggact tttaaatcat ttaaaatact ttatgccttg attttacgaa
     2161 atgaatttaa catttccaac gctcaaactt tctgttttag attttactat tgattgcaaa
     2221 atcaaattct tgaaaaaact tttagtcttg cttgcaaaat attatgaatt tttccgagag
     2281 agagagttat actttccaag atttgattta accttttcgt ttctttttta ctatgacttt
     2341 caatgagcaa atttaaaatt tgtatagcag atgatcatcc tgtggtaaga gaaggattgc
     2401 gcgttatact tcaggcagat ccaagattta aagtcgtagg tctttttccg gatggtaaat
     2461 ctctactaga ttttttaaaa ttgaactctt gtgatttgtt aattttagac attggaattc
     2521 ctggtggaga agattttcac atcttgaaac aagtaaaaag caatacacca gagatgaaag
     2581 tagttatttt ctctatgtat gaaggagcac aatttttctt agaagcaaaa aagcacggtg
     2641 ctgactctta tgttttaaaa aaagattctt attctttaat gcctagtatt gtggagatga
     2701 ctctaaatgg agtttcctac tgttcagaag agttaaaaag atataaagat tcttcagatg
     2761 tgaaagtgac tctcaataca attgaagaga aaatagtgaa aggacttgaa cagaatcttt
     2821 cctataaaga aattgcagag tcgattcaaa aatcagaaaa aacagttcag agctacattt
     2881 acaaaatgag aaaaaggttt caggttcaaa acaatacaga gcttttaatg tttctataca
     2941 agaattactc ccatccttaa aaaactgatt tttattttaa atcaaatacc tcagattgag
     3001 ttgacttaat aacaccttgt tagagtgttt tttgaaatgc catttacaat gtatttgcat
     3061 cgattgaatt ttattctact atttctgttt ttgggaatcc caaatctttt aaatgctcaa
     3121 actttttctg tagagaaaaa gatgaacggg attctgattg aaaattatta tttccttgcc
     3181 gatccgaatg caaaactaaa tatcaaagac gttattctat ccgaatcttt tagacctata
     3241 aaagaaaaaa aaagtttagg gattgagcca ggtgctattt ggcttagaat cgatttcgag
     3301 aattcgtctg atcgaccaat tgaatggatt ttgcaagttg tttatcatca gtttgatttt
     3361 gttgattttt attctattca tgacttagaa ataaagaaag agctacacat tggcgaccat
     3421 cgtctttttg aggaaagatt gattccgcat gggagcaact tatatcctgc aaagatatct
     3481 cccaaagaaa aatgtagaat ttttattcgc ctagcctatg aaaaagaagg tagatttaat
     3541 actgctttga aaatctggac accgaaagaa tttcaaaata actcgaattt ctatcatatt
     3601 ttctttgcag gttcgatgat tggtgttttt actttagtct ttatcaattt attcttttac
     3661 atctcaacaa gaaccaaagc ctatatttgg tatatactct atcaactatc gattatttta
     3721 atgggttgta gctttacagg aattggatac tatgtaatct ggaaagattg gatttggttt
     3781 actgatctaa tccctacctt atctcctgca ctggtattta tgaatgcggc tacattcaca
     3841 atcagttttt tagaaacaaa aaagttttca agtaagatcg atctgttttt aaaattactt
     3901 gcagtgttac atatattacc tatatgcgga gccattctag gatacaaagc atttgcttct
     3961 gatttggtaa tgtttctaag tagtttctta gttttgtatc catttttagg atattacttt
     4021 tggtataaag gaaaacgaga ggctcgtttt tatgcgttag cttggactct atggactaca
     4081 gggatgctta ctggactctt tcgtatagca ggtaaaattg aatctacttt tatatttgaa
     4141 gtaattccat ttctaggagt aattttagaa gctttatttt tcagtcttgc tttggctgat
     4201 cgaattaata tttttcaatt cgaaaaagaa gtcgcagaaa agaaatacac acaatcactc
     4261 gaaaaattca atgaagatct cagtctgaag gttgaggaga aaacaaaaga attacaattg
     4321 aaaaatcaaa tacttgcttc gatgaataaa gataaggata catttttatc ccttattgcc
     4381 cacgatcttt tgggtcccgt tgattctttt aaaatggcaa ctcagatgat tcagtcaaat
     4441 agaaagaaag tcaaattgaa ggacaaaaaa aattttcaaa agttgcttca aagttctgca
     4501 gagaatattt attctcttct agaaaatctt ttagagtggg caaaatcaca gaaaggaatc
     4561 atcgtatttt cccctaaaga attgaagtgc agggagattg ccgaaaaaac gattcaacta
     4621 ttaagaccta acgctcaagc taagcaaatc ataattgaaa ataatattaa aatcaatctg
     4681 tctctctttg cagatgaaaa cttgttaacc actatttttc gtaatttaat ttcaaatgca
     4741 attaagtttt ccatcgaagg aggtaaggtc attgttggta tttctaaaaa ttctccgaag
     4801 gaaattactt ttttcgtgca agataacggt gttggagtcg aaaaagaaaa attaaagagc
     4861 ctttttgagc caggtgttaa cgcttccgag agaggaacta aaggagagtc aggtgttggt
     4921 cttggcttac aaatctgtaa agaatttacg gaacttcatg ggggaagaat ctgggtggaa
     4981 agtaaacttg ggaagggaag tatttttttc ttcaccattc ctaaaagaac tacttcttcc
     5041 ggataattat aattatcccc aagattgtta ttggtatcgc aattaggttt acattgaccc
     5101 aacctagaag ttcatgtaag gagccagagg ttgcaatgga aatggcagag aaccctgtta
     5161 caacaaaatc atttatcgct tgtgcttttg gttgatcttc tggatgaatt tggtaggaaa
     5221 gtagtgttgt tgctcctaca tacaaaaaat tccatccaat tccaagtaat atcagtgaat
     5281 aggcaaagtg aagaaatgta tggtctataa aattgatccc aatacaaatt aaaaaaagga
     5341 aggtgccgat gagcatgatt tttctatccc caaatctttt aatcagactt cctgtaaaaa
     5401 aagaaggaac aaacatacct aaaatgtgaa attgaatgat ctgcgtgatg tcattcattt
     5461 caaaatgatg atgcttcatg ccaagtggtg ttgcagtcat gatcaaaacc atgacgacat
     5521 acgctatcga tccaatacct ataattttag gaagaggggt atgtagaaat aaatccttga
     5581 tggacttact ttttttccag tcgagtgtag cctgtttgcc tgtatttaaa aagagaatta
     5641 cactaaatgc aataaaataa agcggatagg agagaataaa tcctcctatg aaatgtaaat
     5701 ctttccatac actaagaccc catttataaa gattcggtcc gatgagtgca gcaatgacac
     5761 cgctcgctag gacaagggaa actgctctag acttaaaatc tctatgcact acttctgcag
     5821 ctgcaaaacg aaaataggta gaaaaagaac tttgaaatcc aataaaaatt gtcccaagac
     5881 aataaatgat gaaattcttt tgagatattc cgaggataac aagacttatc cccacaagag
     5941 cgaataaaat tcctaagata aatccacttt ttctgccaaa tcgtgccata tagaaagagg
     6001 caggaattgt aagaaggagg gttgcaattg tattgaatgc gactggcata gtggaaagag
     6061 tttgacttcc agtaatttcc aatccaatca tggtatttgt ggaagtcata agtatacttc
     6121 cactttgcac aagagcctga cagataaata taataaataa atttttatac atctacaagc
     6181 tcttcatttc tattattccc agtttagaat ttctttttct agttgagtaa aaatttcatc
     6241 atacgctttc actactggac tatttttatc tgcatggacg attggatttc cattttctcc
     6301 tgactgcatg atttccatca tcataggaat ttttccaagg agtggagatt taaatgaatc
     6361 agctagtttt tgtcctccgc cttcgccgaa gattgcatac tttcgatctg gaagatctgg
     6421 aggaatgaaa taactcatat tttcaatgac tccaaggatt ggaatcttaa cttgattaaa
     6481 catagatccg gcttttgagg cgtctaacgt tgctacggct tgtggagttg ttacaatcac
     6541 tgctccattt atatctatta attgcgcaag cgatagttgc acatctccgg ttcctggagg
     6601 taaatcaatt actaagtaat ccaattcacc ccaaatcaca tcgtataaaa attgttcgag
     6661 tgctttgcca agcattggtc ctctccaaac cactggttgg tttggatcca ctaaaaagga
     6721 gaaagaaata attttaatgc cgtatttttg aactggctca attctatttt catctgttcc
     6781 aagagcgact cttccattga ttccaaaaag ttttccaatc gaaggtccgt aaatatctgc
     6841 atctaataga ccaactttat aacctttgct tgcaatgagc atcgcaagat tagcggttac
     6901 tgttgatttg ccgacaccgc ccttaccgga gcctatcgcg ataattcttt tcacgcccgg
     6961 aattctattc ccgtcatcaa attttaaatt tgggtctaca tcaaatttaa ttttaatttt
     7021 tccaatattg gaaattttag atacggactg acggatctgt gcttccagac caatttgtaa
     7081 ttttctatct gcagtaggtg ttttgatttt aattaaaaat ccatcatctg ttggattgat
     7141 atcaccgagc attccaagag agactaaatc tcttttttgt tctgggtggt ttactttact
     7201 gagttctctt ttaatttcat caattatcgc catttgaaat cccttttttt agatcttctt
     7261 tgtttaatta tccattgatt caaataccct tgttaagcaa aggttttttc tactagtctt
     7321 acatgcctta taagcctgcc tgaatcagtt tctatgagag taatagactc tagattttct
     7381 aagcaaacaa tatcagtaaa aaatcccatt ttagagctta cgaatcatta gccgcaatat
     7441 agaaaatgat ttttcagttt actgcttatt gcaaaagcga tgaactccct agataaatca
     7501 ttcgctagca cgattccatt ttccaatatt ctatgaaaga ggatggattg gattattttt
     7561 gattgcatag atttttgatt aattttatta aaaatttatt ttatttagag cacactaagg
     7621 tgagagaaag acgggaattt agcgctagcc gcaataaaaa ttttatttct aaattagatt
     7681 gtatctatac ctaagtaatc ggcagctttt ttcagataag agtcaaatgt ccataagtta
     7741 caattctgga ttcttgcgga ggcaagcaaa tgaatatcta caaaaccaag tcctctacca
     7801 aataattttt cttctgcaat aaatttgcgt aattgtattt ccgagagaat ggagacattt
     7861 acaaaatttt ccattcgtat ttgtatatct ttttttaatc cacctagaat taattcagta
     7921 agaacgaatg gatgcaaata tacgtcacct gattcaatcg aatctgacaa ggcaatacta
     7981 gatttgctcc cacgaaaaaa ttctacccat acatttgtat cgacaagaat cattatttgc
     8041 gaactcttct gggtgatatt aaattttttt gagatccaaa aagtttagct agttctcttc
     8101 gctctgaggc aagaatcaac tcacgcaatc ccatttcaac aattgctttt ttagttttat
     8161 ctggatataa ttttaaagct tggtttaata agtctttatc taaattcaat gtagtattca
     8221 tacataacat tctatgtatt cttatgtata aagtcaagca ttgtgcgaat tgagcagccg
     8281 tgtttcaatc aattgtttac tatttatttt tctatattta atcttaaccg gaattaaatt
     8341 tcctttgtcg taacgaaaag aagcagacgc acgaaattct tttttataaa agaataatcc
     8401 gcttcatttt gtcagcagag aactttttca ataaccctaa aacctaactg atttcttcta
     8461 actctctttc aacgagtttt cctggaaatg gatcgctagg gagaatttct atttttttga
     8521 gataccttac ttccgtgatc tctccatttg tttcaacaaa ttttgcattg gaataaataa
     8581 ttcttccatc ttcatataca tctacatctt caatgataac ataactctta gcaggtatat
     8641 ctcttaatct aaatcttgga atagcggatt gtatcacagt atccacatct gaaaaaaaac
     8701 tccccgtgct atacgaaatt tctttaaacg aagtattgct ttcatttaca aatgaaatct
     8761 tgcctttgag ctctgctata taaatttgca tattatctat aatctttttt ttcctttcca
     8821 aattcatttt atttttcatt cattttttag ggaagaaatg tttgcgctct ttgcaatgct
     8881 ttgtatcttt ttcaaaataa agaggaaagt tttagagacg ttatgcaatc tcttcgaatc
     8941 tagatttata ttcaggatga gttgtattga atagctcatc ccagatggtt gtataaaaac
     9001 caaaattgta atctaaattt tgatggtgca gaatatgaaa cgttcctgtt gtgattttag
     9061 agagaattgg aattctgacc caattagatg gaaatggttc tacccctaga tgaccaatta
     9121 aaccaaaaag tgtattcagg tttaaataaa aaatcattcc ataccaagaa gaggagtaga
     9181 ggcaaagcag tattagccat aaaactccga atccgattgt ttcaaatgga tttaatacga
     9241 acaatgtgac tggtttcgga tttttaaatt cgtgatgtgt cgtgtgaact aaattaaaaa
     9301 taaatggatt atgagcgatt ctatgaaaaa aatacataca aacgtccata gataaaaata
     9361 atacaagtgc atcgaaaact ataattggca tagagtcatc tgttttgatt tttataaaat
     9421 gatttttcca tagaacccag cctagtagca aaataattgt attgattagc actgtggaga
     9481 tagaaaaaac aatttctatt ttagaaaaat tttctgccat taactcgtaa gctttattct
     9541 tttgaaaaaa tttttgaaaa attctacctg tgtaaataca agcaacgaaa atcaatagat
     9601 tcagtattga tgaatagaaa atcaattccc aaaatgtcca attccaaacc caatctttca
     9661 tacttttaac agtgataaaa gctttctttt taatttctac cttttttgat tctcaattgt
     9721 aaaaactgag tctagttttt tgtccgaaat aggcttgact tttatatata aaaatttata
     9781 tataaactat gatatatttt aaaaagggaa ctgagattca atgctaaagt atgcgattct
     9841 aggttatttg aattaccgtc ctatgtttgg atatgaactg aaacaagcaa tggatgggtc
     9901 tactagtttt ttttggcact ccaaattgag tcagatttac atgactttaa aatcattaga
     9961 aaaagagaag aaactaaaat cagaaattga aaatcaggaa gataagcctg atcgaaaaag
    10021 atatacaatt actaagcttg gaaaggaaga tttgaatact tggttagaga cgcctttgac
    10081 ggatatcccg caaaacaaag atgaacttct attaaaactc tttttctccg cgaatcagga
    10141 taagaaaagt atcctttcag aacttcgcat ccaaaaaaaa ttacatgaga atcaactcaa
    10201 tacctatata aacgtaacgg caaaagtaat taaagatgct aaaaaagaaa acccggcttg
    10261 tgcaaaggat tcaattttat gggaagccac tcgcagaatg ggtgagttat atgaaaaggc
    10321 ttatattcaa tggatagatg aaacgatacg cgtcgtagaa tcaaaattat aggagagcta
    10381 aatgagacta aaaaaaatat ttctgatagg aattttattt tttgcatttc catcttttac
    10441 agagacaaat ttttcaaaac taatgattcg aattgatgga gtaaaaaata aaaaaggaac
    10501 tgtaaatatt gctatttata agagcgatgc agattttaaa aatgaaaatt ttgcatacgt
    10561 aagtaagatt agctcggatg caagtgtata ctttgttttt gaaaaggtgg aatatggtaa
    10621 atatgcaatt gctatttatc atgatgaaaa tgaaaatcgc aaattagatc gaaatatgat
    10681 tggcattccc tcagagggat atggtttttc aaataacgca aaaggctcaa tcctaggacc
    10741 tcccaagttt caagatacat ccttctcagt agaagagcct cagaaaattc ttacgattca
    10801 aatgagttac taaataagga gcctcccatg aaatatacaa ctgtcttaat tttcttattc
    10861 tttatgcaat gcagtattgc ctctataaaa gaaggcgaag aaaacattgt taaaacgttt
    10921 caaaagacta tagctaaatc aaaatttagt aaggcagcta ctttattagt tcattccgat
    10981 actctaaaag ttcacataga atcgagtgca ggatttttag ataactctaa aactattcct
    11041 gtaaaaaaag atcaaccttt tcatgtagca agtattggta agatatttac aaccgtttta
    11101 atttatcaaa ttattgaaag cggtaaatta aagttagacg atcgtatcag taaagtatta
    11161 agtccagatt tattgaaaaa tttatttgta tatgagggtg ttgactattc agaaaaggtt
    11221 acaatttccg atttactcga acacacatca ggagttgcgg attattttga agccctagat
    11281 aaaaacgata aatccctact agacgaaatt caaaagaatc cagataaatt atggactccg
    11341 aaagaaattt tagattatac aagactcaat aaaaaagcag tgggaaaacc tagtgaaaaa
    11401 tttcattatt cagatactgg atttatctta cttggactta ttattgagaa aattacaaat
    11461 aaaagttttg agaaaatcat tcaagagaaa atcttttctc cactgggaat gaaaaattca
    11521 tatgtttact taagaagtga acctatcgac aaatcaaagt tagccccaat ttctacaatg
    11581 atgctagggg ataaagatgt aacaaattat aaaagtataa gtgccgattg ggcagggggc
    11641 ggaattattt ctacagctca agatttactt ctttttcatc aagcacttat caagggaaaa
    11701 ctcatctcta atctaaatta ttcctccttg gttggcaaaa ataaatttct agatggaatt
    11761 tattatggca aaggattaat gactgttcgc ttcaatgata tgtcgttttt aatgcctaaa
    11821 acacctgaac tctatggtca ttctggcctt ctaggaacgc ttctatttta ttcaccagaa
    11881 tatgattcct atataattgc aaacttaggt agcacagaag atgtagggga aagttttgag
    11941 atgatgtttt ggattatgca ggatttaaaa gaaattaaaa atttaaaata gaagataatt
    12001 atggttagcc tcagattctt taaaacttca aaagaaaatt ttgatcttaa aatattattt
    12061 tatatctctt ttatttgttt tcttatttct tcttttggat ttgtttttga tggtagagtt
    12121 ttaaattata ctcctctttg gttaaagcct ctaaaatttg caatctcaag tttaatctat
    12181 tcaatcactc ttatatattt tgtaagattt attccgagtg aaagatttct ggtttgggca
    12241 aataaaataa cttcctatgg tcttatatta gaattgctta taatctattt tcaagccgct
    12301 cgcggaagaa tgagtcattt taatttttta actttagagg atatggtttt atttcagatt
    12361 atggcagttg cgatcgtttt ggtatggttg gcttttttta tctatataaa aggttttttt
    12421 tcaatacctc taggtagaaa tatattttta ttttctattc gaatggggct aatcattagt
    12481 ttcctctcaa tgccaatggc ttttacgatg acacaaccta gctctgctga cttaaaaaaa
    12541 attgaggcaa ataaaagtcc aattggattc acaatgggtt cacatagtgt taatgaaaaa
    12601 gatcaaacaa agagacttcc ttttacaagt tggtcacgca ctggaggaga tttaagaatt
    12661 gctcactttt taggtttaca tgggatgcag atcttaccgt tgttcggaat gtttttattt
    12721 agatttagat atgattataa gatggggaaa attatcatta gcctcttgtc tattatatat
    12781 tttagtttta tggtttttac attaatgcag gctttagagg gagttccttt tatacgctga
    12841 ggagggtata tggaaaacaa atatgatgtg atcgtgattg gttctggtat tggtgggttg
    12901 gcagcagcta ctctattgtc taaaatagca aataaacggg ttctagtttt ggaacaacat
    12961 tgggagctag gtggactaac gcacgaattt aaaagaaaag taaaatatag ttgggatgtt
    13021 ggtcttcatt atattggaaa tatggataaa aatagccttc cgatgagagt ttataatttt
    13081 ataagtaatg gaaatataaa gtggaattct cttccttatg aatttgaaag gtttatttat
    13141 ccggatatga cgtttaccgt tccatcaaat gagaacgagt atattcaaaa actatccaaa
    13201 tcatttccag atgagacaaa atcgatccaa aaatatttta aagatatgaa aaaggcaacg
    13261 cagtgggcaa ctcttaatat gatggcaact ctagttccat ccttctttgg atttttcatt
    13321 cgcttatgga ataaaaaaac agagagtatt gcattatcaa ctacaaagga ctatttggat
    13381 ttgaatttta aaaatgaaaa attgaaatct cttttagtga gtcaatgggg agattatggc
    13441 ttgcctccct ctaaaagctc atttctgatt cattctatca ttgcaactca ttatctcgat
    13501 ggagcgattt acccggaagg gggagcaggt tctattgcaa aatatgctga gcctattatt
    13561 gaagcaaatg ggggaaaatg tttgttatcc tctatggtta ctggattgat tgtagatcac
    13621 aatacagtaa ttggagtgga agtgcaaaaa aagcgagggg aaaagagaaa agaaatatac
    13681 caagcagaaa ctgtgatttc atgcacaggt gcccttcata cttatggtga attaatgaaa
    13741 ggtcatattt ccgagtcata cattgaagag ttaaagaagt tgccaagggg gaaaagcgct
    13801 gttaccctct atctcggttt aaaaaatgat ccaagagaaa tgggttttaa aggggaaaat
    13861 agctggatct atgaatccta tgatcacgat ttggaagcgg ctaatgctgc taattcttta
    13921 cttgctggaa atccaagggc ttgctatctc tcttttcctt cccttaaaaa tcattctgca
    13981 aaaggtttta cagctgaaat tatatctttt gctgattaca atgattttac tcaatgggaa
    14041 aactctaagt ggaaaaagcg tggggatgat tatgaaaaat taaaatcaaa aattgcagag
    14101 ggtctattaa accttgttga aaaaaaatat ccaggattta aggatttggt agattataca
    14161 gaattatcca cgccattgac ttatattgaa ctcgccggta gatcgaatgg agaattttat
    14221 ggactcgctt caacaccgga gagatttaaa attaaatggt taggaccaaa aactccttta
    14281 aagaattttt atttagctgg cacagatgca atggcgttag gcatcacggg agcaatgatg
    14341 ggaggtgtag cggctgccgc atgtgtttta gatccaggtg gatttccaaa aataataaag
    14401 ggagtaaaca gaaaagtata attatggctc cggaaccaat ccttactttt ctattgattt
    14461 gctgttctag ttttattgga cctagaacag gggtgtatga tggagaattg aactattgtc
    14521 cacctacacc aaattgtgtt tcctctcaga gttggaaatt gaatcctatt cacagcatcc
    14581 gaccgtatca atatgaaaat caaacaaagc aagaagcata tgatcaattg aaaaaattat
    14641 tagatagcaa agagaatgtt catgtaatgg cagatccgga aaatttttat attagaactt
    14701 attatttcac aaaggttttc agatttccag acaaagtaga atttttattt gaggagaatc
    14761 aaccaatagt tcaaatacga tcagcttctg tgtttggaat ttttgatata tttcacaatc
    14821 gaattcggat ggaatacatt cgcaaggagt taggctggct ttaaaaaata cttggcatac
    14881 aaactaaact ttttaatttc taattagagt tcgattaatc actccaatga ggtaaaaaat
    14941 gtcatctaaa ccaccagtaa tcaatacatc cagaaaattt tcggatggaa tggcttccgc
    15001 acacagtctt ctaattcaag ctaaaaatgg aattaaggga aatcaaaaag gagaacgata
    15061 tgcgattcct gcttttaacg taactacatt tcaatctatt aatgctgcgg tttctgctgc
    15121 tgagatgtta ggtgcgagtg caatcttagc tttttccaat agcgctttaa aatcatttgg
    15181 tttaggtgat caaacctttg gtgttgaagt aacttctgaa tatgttgcaa agctcgctaa
    15241 acgatcaaaa attaaaatag caactcattt agaccatggt gattatattt ccgaatctgg
    15301 tcgtaaggtt gtgcaagcgg ctgttcaagg ttatacatct gtgatggcag ataattcaac
    15361 agatcacaaa gcagaaagag caatgccttt gagtgataac attgatctta caagagaagt
    15421 cgtaaatatg gcacaccctc tgggactatc ggttgagggg gagttgggag ttttggctgg
    15481 tgaagaagat gaagatacta agtctgacca tagcacttat acaaacccag aagagttaga
    15541 acaattcgta aaaggcactg gagttttaat gcttgcgcca acgataggca ccatgcatgg
    15601 accaaataaa ggaaagcctg gacacaaagt aaaactaaat attgaactag cacatcaact
    15661 tttgaaaatt gcagattcta ttaatccaga aattttattt gtggcacatg gggcttctac
    15721 tctttatccg gaagtcgttg attttgtttt aaaaaatctt tctccttctc atccatccgt
    15781 tcaaaaatgg aaagactttg taggaactga ttgggatcaa atccaaggat tgattggtgc
    15841 tggttttgca aaaattaata cagatacaga aaaccgtcag acctatgtcg cggctctcat
    15901 tggagcgctt aataaagatt ctgctaagat tgacatacgt cactatgata aagttacaac
    15961 agaagccctt acacaaagct atattaaaaa gtttatacta gcgggtgact atggagtttg
    16021 gtatgagcca aaaatcgata taaataaatt caagtttgac ttatctatcg atttagcaac
    16081 tgcaacagga acaggtaaat aaggattatc cgctaatagt ttgaccttac tcaaaacaag
    16141 ggaaaactaa ttgccatcga aggtcaccgg taaaaaacga tttgaaagga agagtttgaa
    16201 ataaatatcg taatttttat cggtgaaggc tattgattct aggaatatat tttaaaaact
    16261 caccgagtct aattcataag gaaacaaaac tttgcaaaag attaaattta caaaaatgga
    16321 aggcataggc aatgactatg tttatatcga cgcaacaaaa gaaaacatac gtttaacccc
    16381 tgatcaaatt caaaaattgt ctaatcgaaa ttttggtatt ggaagtgatg gcgttatatt
    16441 tattcgcaaa tcagacaaag ctgattttat gatggatatg tataactctg atgggtcttc
    16501 ttctgaaatg tgcggaaatg ggattcgatg tgttggtaag tatatatttg attataaact
    16561 tacttctagt aaaactccaa aaattgaaac cggtaaaggt gtcttggaac taaatatgga
    16621 gactgacgca catggaaaag taaatatggt gactgttgat atgggggaac cgattttagt
    16681 tcctgaaaaa gttcccgtat tacttccagg cacagatcca atcatcgagc atacgattga
    16741 tataaaagga tataaaataa agtttactgc tgtaagtatg ggaaatccac atgctgttat
    16801 atttgttgaa gattcagatc agtttccagt aacggaaata ggtcctattt tagagcatca
    16861 tcccttattt ccaaagagaa ctaacgttga atttgtttcc attcgaggaa aagatcatct
    16921 ttatcagaga acatgggaac gtggtgcggg ggaaacgtta gcttgtggaa cgggtgcttg
    16981 tgctgttcat gtagcagcta acctaacaaa tcgagctggc aaaaaaacta gaattgattt
    17041 aagaggtgga actctctata ttgagtggac agaatcagga agtattataa tgaaaggtcc
    17101 tgctacaact gtctttgaag gtgagttcca aatttaaaaa tctataattc tataaaaata
    17161 ccatttctgg tttgattaaa agcggctaat atcaattagc tgcttttgaa atctgcaatc
    17221 attttattct gaataaaatt taaatcctat tgtcgatctt ttctttttta tttctctgga
    17281 aaaacgagag taaaaattaa aaggagtttt tatgtatcgc tttttcatag tatctattct
    17341 tttttctggc ttatttatta attccatttt atctgctaaa tgttattctt ttcgtaagat
    17401 agaatctatt aaggtttgta ttgaagggga ttcaaatgct gatagacgaa aggcgcaaga
    17461 tgtttgtaaa gatgttttgg gagctgattg tggtggagtt gcgggctata ctggttcttg
    17521 tgctaaatca gagaaattaa aatgttacaa taaggatgca aaagagcaga aacatataac
    17581 tgttgattga atctgctctt tgcttttagt tattcagtaa gaacacttgt gttatcattt
    17641 ggtattctgt ctgcttcatg atccaaccat ttgttcgtaa tggtcgcttg tctttttgga
    17701 tctttaaata gagtaattaa gtagtttgtg atggtgtttg tgccttcttc ttctgcgtct
    17761 ttatccattt gtttaaaaac gtcaatgatg tcttggtcag gccagttttc taatagagcg
    17821 atagcctttg gtggtggcat gttcgcaatc ttatttgcta gaactttcac ctttgcctct
    17881 cgcttctcca aatctttttt tgccatcgcc atttgttttt ctttttcttg aatgctcttt
    17941 ttcatttcat tcaatttctc gttttcattt tgaagctttt ctctttcttc atctaaagat
    18001 tggcgaattc tctctatctc tgcaatttct tcatctaact tttctcgtgc ttttttatat
    18061 tctaattttt caacttccgt tggcgattct ttatcccaat tgacaggact tggtttcttt
    18121 gcaagagcag gaaaaatttc atctgcatca atgagcccat aataatcaaa aataaaaaaa
    18181 ccaaccgtta gtagaaataa aattaataat aacagatata aaattctcgc cttgtcggcc
    18241 atcgttccca tagatactcc ttagttataa tcgtgtattg ctttatcgag tctaaatact
    18301 gccgcttcta gttcatttac cttatttgta agtctgacaa gtgagctttg aatttttatg
    18361 tccttgggag ccttttctga attaatatta tataaaaggc ttacaaaaga gtttccttca
    18421 tcgcttttgt ttgagagatt cggcatactc catgagcttt tcatactctg atttaggttt
    18481 acttttatcg gacttaactg tcttaatttt ctttttgatt ttaacagtag gtgtttcagt
    18541 tttctcatct ggttgttttc tcccgtgaat aaattcattg tttgtgacct cttcctcttc
    18601 cgatctttcc tgcttaagca ttttctcatg gaattccttc catgcgtctc tttttaatat
    18661 ttctaaaact tcagcatctt tttctacttc aatgactctt tgtctagcct cttgcaaaat
    18721 ttcttgttgc tcattcatac ttttctgtaa ggtttcattt tgcaaatata agctttttat
    18781 atacccttca aataatctta aagttttaat gtctgaccca gaatactttg tagttgagtt
    18841 tagaataatt cgattgttat ctttgatttc attttgaaga cggttaagat tcccaacgat
    18901 gatggaaagt ttttttaact cttcatccac tctaattttt ctaagttcta ggactgtctc
    18961 taaatcaaac tcaaactttt tcatgtttat aacaactcat catctacagg cgttaagaat
    19021 atatctctta attgataaag catatcctga aagtttactt tctgctctat cttctgagtt
    19081 aagaattcat ctatcattat tttcttactg atagctaagt ctgttttggg atctgcacct
    19141 tttacatacg catttaacat aatcagtttt tctgattttt tgtaggttgc aatcaattct
    19201 cttacaaaat cagaatacat tttatggtct tctccaacga tatacggcat cactcttgat
    19261 aaagaagagg ggatatcaat tgcaggataa tgatttctct cagcaagttc tctagataat
    19321 actatatgac catcgatgta acctcttacg gcatcggcaa ctggatcatt catttcatct
    19381 ggttcggtta agactgtata aattcctgta atacttccgc ccgatttgga tgttccagat
    19441 ctttcaatta gttttgataa tttagaaaat accgatgcag aaaaaccacg tgtgattgaa
    19501 ggttctccat aagaaactgc aatctctcta tttgcctggg caaatcgagt gagagagtcc
    19561 atcatcaagt ttacatgcaa accttgatct ctaaaatatt ctgctacaga tgttgcaagt
    19621 aacgcacaat ttacctgttg cattttgggt gcgtctgatg ttgcaataaa tacaaccgtc
    19681 tttgccattg cttcttgccc aatttcattt tctagaaatt catttacctc acgacccctc
    19741 tctccaacga gtgagattac attcacatct gcatttgtat atcgtgcaat cattcctagg
    19801 agtgtggact taccaacacc cgaaccagaa aaaattccaa ctctttgtcc acgtccaatt
    19861 gtaagaagtc catcaatggc tctgactcct gttacaactt gttctctgat gattggtcta
    19921 tcgagtggat ttggagtttc attttcagga gacctttctt ctcctgttac aataatacct
    19981 ttaccgtcta tcggtcttcc agctccattt aaaactctac ctaaaagttc tttcccgacg
    20041 tgaatggtta gtttttttcc tgaagaaaaa acaaatgctt tcggaaagat tccactgact
    20101 tcgcccaatg gcataagtgt atatttgtga ccatcgaatc cgataatttc acattgaaga
    20161 tagccttttc cgtcttgtct ttcaatttcc atgatctcac cgatcttcga atcaggagga
    20221 ccttgtgaaa aaataacgtt tccaagaacc tttaaaactt tgccactttt acggattggt
    20281 tctgtcttgg aaacgattag cttatactta gaaagtatat ctattttttc tgtaaatttt
    20341 ttctcaatca tggagtgtaa ctactatcgc ttagttttag attggttcag cgtttcgaat
    20401 tgcctcttca atttctttga gctgtgtaga aatacgagca tcgattgctc caacatctgt
    20461 ttcaatgata acaccaccac gatcgatccg tgagtcttcg aaaatattga cctttcgtaa
    20521 agattccata agtttaataa gttcatcttt atgtgcggtt gtgatttcaa ggtcagagaa
    20581 gttgacacga atatccacac gatctctgtc tttaatacgt ttgagtgctt cacggatgtt
    20641 atttagaacg atttcttttc tctcaatgat ttcatcctta attactttac gagcaatgat
    20701 taaaatcatt tcgaccattt gtttttcaga agcttgtata atgtcttctc tgatatcaat
    20761 tgcctttcca acaattgttc caagtcggtc aatgagtcgt ctaacttctc cttgtccttt
    20821 tttgaaacca acctcgcgac ccgcatcgta tcctctttga tatgcttcat gttctatctc
    20881 agcaactttc atttccgctt ctttgatcat acgctctact tctaattttg cgcgatctaa
    20941 gatttgttct gcctttgctc ggccggaatc ttcttcggtt ttaacttttt ctttggagtc
    21001 ttggatgagt tggaaggctc ttgtctttcc atcttccaca attttctctg cctttgcttc
    21061 tgcatcggca agcatttgac ggacttgctc ttctgtttct tgacgatagc gatctaattc
    21121 agcttcgatt tcatcgatag aaggaccttg gtattgctca attatattcc cttcttggtc
    21181 tacttcaaac tcttcttctt cctctgcttt gtgaaatttt ttatatttct cagggatcgc
    21241 aagttctaaa gcgtcattgc cagattgcgt ctccgcaatt tgcattggtt tgaatactaa
    21301 tttagccatt gattaaccta ccttccataa tttaatagtg cctttgtcga ttgattcatt
    21361 gagaagtgtt atgattgctg tttgcgcttc ttcaatttct gctaaagaga ttggacccat
    21421 tgaatccatc tctaaactaa tttgctttgc aagccatggc tcacaacgtt ctataagttg
    21481 atcttttacc gatgtttcaa ttcctttgaa agaagaagcg atcacgattg ggtgaatctt
    21541 agaaagaaat tctgttaaag aagaacggtt tgcatattgt aagtcttcga ttgtataaaa
    21601 atgtttgttg atgttttctg caaagtctgg attcttcttt ttgattttgg aaaatagact
    21661 gaaagataca tgtggactta cacgacttaa tatctcggca gtaattttac ttcctctatt
    21721 ttttacttta gaaactgttt ggttgttttt gataagttca tatttaaagt ttaaaaatct
    21781 ttctagttcg tttcgaactg tttcagagtg aaaatcaatt ttatgaattt cgtaaataat
    21841 ttcttctctt ttcttttctg gaaagtcttc tatgagagaa gatgcttcgt ctggatttgc
    21901 aaaactcatt acaagagcga tgagtctagg ctcttcttca tttacaatga gtgcaagctc
    21961 ggatcttttt ttcttacgaa gctctgctat agctgatact ttattgttag ctgtttcttg
    22021 gataagtttt tctatttctt ttgcaatctt tgcttgcaga gcagaattag aaactcctct
    22081 atatttatta aaagtatctg caaatttaga taaaacattt ttctcttcat ttgcagaggg
    22141 ggcttttagt gccgttactt tttgcaaaag cttttctaat tcatcttgag ataattgagc
    22201 gaggacttct ttaggaagat gatccccgat cagattatac aaaattgcag ctttactgtt
    22261 ttggtttttt gtgcgggtca acctgcctcc tcttcagaca gccatgttct gagtaattgt
    22321 gctacatctt ctggcttttc ttttgctagg ttgatcgcat tttccaataa ttcttttctg
    22381 agtttttcgt cgagagagag ctccacatcg gctcctcctt cttccataac tcgaagtgct
    22441 gcttctctca tcatttgttg ttgagctgct aactcttctt ctctgagtct tcttctgcga
    22501 gcgatttctt tcttgattgc tctgtaaatt aagataacta atattaagaa agtgagaatt
    22561 gcaagagaga tgataattgc atttcgaatt gctttttgtc tcgctaattc tgcatcttct
    22621 gctgcaaact gagcagttct atctcttggg atagacataa cgtttacaac atcccctcga
    22681 gttttatctg ctccaatggc tgtttctaaa ttctttttga ttttacgtaa atcttcgtcc
    22741 gaaacaggga tataagttct atcaaatttg tatccatctt cggattcttt tttagtccag
    22801 attccgtcta tcgcaacagc gagagtaatt ttatcaattt tccatttttg tttctttaca
    22861 tctctcactt tacgattgaa ttcgtagtta cgaatttttt catctttttc gtaatttgct
    22921 ttttgatagt caacgtcttt gtatccagga gggatatttg gttcagtccc tgccggacct
    22981 tctggagtaa atccacgtcc attgaatttt tcagaagttt ctttgtcgga aatttttaag
    23041 gaatatccgt ctactaattc agtttcatca tatggtgtat ttggattgtc tgcttttgct
    23101 ataacagggg aaacaagatt ttctttaaat gattcttcat cccaatttaa atcatattca
    23161 aaacgagtta catctactcg gtcttctccc gctaggagcc atcggagagt gttgcgtata
    23221 tcgactagtt ttttaacacg ttctgcatct gctatacgca ttttctcttg aatgagtctg
    23281 agctcaattt tttctttttc taaatcttct tcgaagtcac tgataatctt tccagttgag
    23341 tcagctacac ttacatcttc tggttttaat tttggaactc cgcgagaaac taaatttaca
    23401 atccctttga tctcttttct ggaaagacct tctattcctg gaacaaagtg gagaataacg
    23461 gcagctttta cgggagtggt agaaccatct tgcgaaaaat attctgtttc aggaaaggca
    23521 atgttcacat ctgctttctc aatgctttgt agagtcatga gagatttttc aatcgcacct
    23581 ttgagagctc tatatttctt aacgtcttta tcaaattgag tttcagtgaa tttttcgata
    23641 tcgaataatt cccagccttg cactccaaca gggattaaat tttcttgtgc aagctttgtg
    23701 ataatttctt gtcttttctc tggatccaca gaaatcactg agtctccact tcctgaatat
    23761 ctataaccga gagcatctaa tttttttgta atttcaccgt agtctttttg ttggaggtct
    23821 ttaaagagaa taactgcatt tttttctaga gagaaagtgg atagaataat cagtgcgatc
    23881 acaacggtcg caaacacagc tcctagaatg atttttttag tgctatctag tttatcaaat
    23941 agctctttga tttgggcgat aactttttgt aaagactcgg gcataagtat cctcggaaat
    24001 tattctctat gacataataa gaaatcgaca gaaatgtaca attttttttt aaattttctg
    24061 taaatttttt cgatttttga aaaagtaccc tagaagatgt tcgatgtcaa ctttattctg
    24121 atattactgt tcagtggagc aattcttttt gcttatgatc gtcactataa tacacccttg
    24181 atctatgtaa aagaggaaag agtttatatt gccaccatct cttttggttt tgcggctgtc
    24241 ggctcagccc taggaatcca aattgctctt tctaagttgg gatttcagga aaatatccta
    24301 aacaatgctt ttatttattc agcgttagtg gaagagagtg tgaaatgcgt ctttatttta
    24361 ttggttcttt cttacaatag ggtaaatgag gtgttatatg atggtcttta ttatggacta
    24421 gtatttggcg gggcatttgg tctgattgag aatataatct atgcatctgt tctcccattt
    24481 tggccaatga tgcttcgtac gattacatcc tccgctcttc atttattaaa tggaggaatt
    24541 atcggttact tcaccgcaaa atacctgttt accgcaaaga gacatagatc gtcctatttg
    24601 ctacaaggtt ttcttctatg ttatgtctca catgctctct acaacctagc aggattcact
    24661 ggtggaaaat ttttaatgat tctaccaatc ttattagtat tcaattttat cttagtagaa
    24721 tttatttcag cgtatgctag aagttctctt cctaagtttg cattagatct gattcattta
    24781 tccattttgg attatgaatt gattcgaagg catactaaat atgaatcatg gctttacaat
    24841 gagcagaagg tttttaagaa aaaaacgaac ctattccaaa aagtgagttt gaatcggatt
    24901 ctttctgtca gcactgcttt tctaattagt ttgtttttta taactctcta ttttttattt
    24961 ccggaaatta gaagcaaagt ttttaaagag attttgtttt atgaatatgt gagtattttt
    25021 attaattatc ctcttatggt ttcgattagt attttatttg caggtcttgt gaatcctgat
    25081 ttttttcaaa gagaaatact tcgagtgcca cttatgtgcg tgttgagtgc taaatcgaaa
    25141 gactatgaag agacgactgt cgttttttat ttaacttttc atgggtttta cgctcccttt
    25201 atgtatccag aaaaactgga aggagatttg ctcttaagct ttcagattgg aaataaaaat
    25261 atagagaata taaagggtaa aacaatttgg attcatggag caaataatga aacacagaat
    25321 ataaaatctc gtctatcggt tacaggtgcc ttgattcaat ttgagaaaaa tccttttttc
    25381 ctaattactt attggaattg ggcaagatgg gttttgcgaa tacaaaactt actaaggctt
    25441 gtttgatatg attgaaattt tatatagaga tgaatttcta ttagccgctg taaagcctgc
    25501 aggtattcct gtgcatgaaa ccaaagaccc gaaacgggaa aatttcaccg gtcttgtgca
    25561 aaaagaatta tctcttcctt atatcagaac agtcaatcga ttggatttag agacaagtgg
    25621 aattgtttta tttggtctta cagaagagcg aaacaaggaa atagatgaga ttttaaaaaa
    25681 tgcggataaa tactatttgg cgctagtgga aggaaatcct ccggatgagt ttcgcattga
    25741 atcttttttg aaagatggaa acaaaagagt ctcaacggtt cgaagtggtg ggaaaaaagc
    25801 aatcactgaa tttaaaacac ttgagcgttt aaatgatagg ggatattcgt tagtatccgc
    25861 acatttaatt acaggtcgaa gacatcaaat tcgaattcat ctctttgaaa atgggtttcc
    25921 tattctagga gataaagtct acacaaatcg taaaactccg cttgcgaatc gatctatgtt
    25981 acatgcatat gagttacact ttcaaaattt atttggtgag aatatacaaa tcaaagcaaa
    26041 accacccaat gactttttga aatttttgaa tagatagatt tagaaaaagc aaccagaact
    26101 agtataggat tttatcattt agcgaaatat tgaaattttt ttttaaaagt tcaatagatg
    26161 aatattagag aattctaata atccaaagta ttagtccttg cattgaaata gattgaatga
    26221 aattgagaat aattaatttc tctttttcct ttgtttactt gcccaatcaa aaaatccttt
    26281 tttaaatttg ataaaatttt agataaagta atgttattat tcaccactta tagtttattt
    26341 tatttagagg ataaatggtg ttatcctcta aatggttttg agatgcaatt atgttttgga
    26401 taaataatat tatcctcact tcaaaaaaat atatttacat atcaaaataa aagagcatac
    26461 tgcttctatg aaataatact tgtgaaatat ttttttagaa ggaggaattt atgaaaggtg
    26521 ttattgtaat gtgcttgaaa gaaatgatag agacaaatta tggatcggat aaatggatag
    26581 aggtattagt aaattcgaat gtagacacta aaacagcctt cattgctttt gctgatattg
    26641 atgacgcaat tgttttaaaa atgcttggat ctgcatctaa aatattaaaa attacatcta
    26701 tacaagcagc tgatgcattc ggagattact gggtgaatgt atatgctcaa aaaatgtatc
    26761 gtagtatgtt tgttggcaaa aaaacagcaa aggaattttt attatcgatg gatcaacttc
    26821 atgtaggagt gactcaaaat attaaaaatg cacatcctcc cagatttgaa tatgaattaa
    26881 agggtgaaaa aacacttatt attaaatata aatctgatag aggtttgata gatatcttta
    26941 ttggattaat caaaggtgtt ggaaagttat ataaagaaaa tctacttgtt tccaaattag
    27001 gaaataattt agtgcaagtg caatttaatt aacttaatga aagaagatac tgagaggtgg
    27061 ttgtgaataa aaagattcta ctcatctctt tttttttata ctttaattgc tataatgaaa
    27121 atagacaact aaaaccactt tcaaataaat atttcctaaa gacaatagag gctaacgttc
    27181 ctattgaact aaaagagatt caaattttta gggaatggac gaatgaaagt tatcttaaac
    27241 ctcccaattt aaactcatat attatttatg tagacagcga aaaatttaaa atttatttta
    27301 aagaaaaact gcaagaaaaa atagcagaat caaatttagt ccaagaggat aatgtaaaag
    27361 ttgaaataga aactttttcc ttatcgtata aaattgtaaa agatttcgga atcataatcc
    27421 ctcaccaaat tatcacatcg aacatgcaag ttagaacgaa agttaccatt ttaaatcgcg
    27481 tagaaacaat cgaatacaca aagaagcttg tatctgaaag taatttaggt tcgccatttt
    27541 taattttcta tagcttaaca gctcttatga gaatgggaaa tgtagtatta ggaacagcag
    27601 cacttggaag tagcatttta acaggtggaa ttttttattt cgatgcaatg tcagataaca
    27661 ttgaacgtta tgtggaaagt gatttagata gatacagcta ttatctaaga aataaattag
    27721 aaaaaattaa aattacaata aaagaaagag aataaacaat gattttgaga atattggcga
    27781 gtgcgagtca ataaaaatag aataattcta aataattctc cgtttttagc gtttgaatcc
    27841 aaaaaataaa tcctaaaact agagccaatt tctgattaga atcagacgat aaatagaata
    27901 aaccttgaaa agttttctag gctgaatcat ataacctgta gggagcaact gatgagtttt
    27961 gatgatcgaa ttttttctaa atttgatttt agtgaggtgg atagagaaaa atgcattcca
    28021 ttgatagagg aaataattag acttgcagaa ctatctagaa agcaagggtt actcttgcta
    28081 gaagaagagt taagcacaat caaagatcct tatctaaaaa tgggacttca attgatcatt
    28141 gatggaacag agcctgattt agtcgaagaa attctagatc gaattgtatt cttttcaaat
    28201 gcatcgccag aaagagttct tgagttttgt attatgcgca ctggtatact atcgattcaa
    28261 gctggaaata atccctacgt attaaatcat caactttatg cttatatagg aattgaatat
    28321 atagaagagt ataattttac tgaacctgag acaagaattc gcgaacttgt tgttaattct
    28381 aattttgttc cccttgatat ttcattagaa tttaattttc ttttttctag attggatgat
    28441 ttttccgtcc agagaattgt tcgtgaaatt gatttaaatg ttttaggatg cgcaatgact
    28501 aatgttagca ttgaatgtga atcaaaaatt ttaagtaata tttccacaag agcagctctt
    28561 cttttgtcgc aggaaagaca atttagaacc attcctttag aagagtgtat cgaaaatcag
    28621 caaatgattc tagaattgta tgaaagactt gtagaaatgg gagaaatcat ccgagtggag
    28681 gagtaattac ttttttcgga aaggtttctt tttgactggt tccggcaaat aaagagtaac
    28741 cgctttccca tttaaataaa ctttttctat ctgaaagagt gtttctagat tctttgttgg
    28801 attctctttt actaaaatta tatttgcctc ttctccgact tggatgagtc ctctgtaaga
    28861 tccacctaaa tattcacatg tatttttagt tggaatcata aagatttcat ttgtatcaaa
    28921 agcctttgct aagattctta gttcttgcat cccacttact cccgcaaagg acaaacgatt
    28981 tcctgatcca ctccctaaaa tcattttctc ttttaaaata ggatgtgttt ttaaaaaatc
    29041 aatatatgat ttaaattctt tttttctggt ttgaatttcg atttcttcta attccaattt
    29101 caacgcactt tcataagaag ctgcaaaata gtctgtaaag aatttactct ttttctttaa
    29161 atagtttaat tcatctattc catcagaatc tgcatttaaa tgtaaattct gatatacatt
    29221 tagcattggc attaaatgca tttcttgtat atgctgtctt gcaatttctc tgttcatatc
    29281 attcgggata ggatgtgcca gataacggtt tccggatatt aatgaatcta gaattgatat
    29341 tctatctcca aatgtatgaa ttgttattct tttatttttt gcttctgagg atagtcttaa
    29401 gtctagcaat agttcggaat caaataagag gctagaatcc tcattgtgtc ggttgaaaat
    29461 atgaattgtt ttattaaacg catttaactg agcatttaat tcagtttgca gtgcttcttt
    29521 gttttctgta acaaaataca attgatttga aatatcttta gtttctttac ttttggctaa
    29581 taacggtcgc tgtgctattg taattttggg tccaactaac tttcctgaat ctatatctga
    29641 ttttactttt tgtatccagg gaccatctgc tattgattga atatgagtga acccaaaagc
    29701 aaggaaggct tttagagatg tttcaagact ctttctatcc tggtctccgc ctgcgggatt
    29761 tactccgagc gttacattcg catcgcaaaa agatggaagt atatatcgaa tcgtatcttt
    29821 gggtttactc tctgttatcc gtgaaataaa accattttct attacaagat cacttacttc
    29881 actcaaagac ccgttaggtt ttaagtatcg tatagattta atttcttcgg aactgagtgt
    29941 gttgaataaa aataataaaa taaaaataac ttgtttcatg cttttgtttt caacaaagca
    30001 cgtcttctta aaagaatcaa gttatttcta gcagttcccc gaaatgcccc accatagccg
    30061 tatttaaatc gaagtggttt tgccgatgtc tcataaattt ctttcaagtc ttcctgtgta
    30121 aatttctgtg attctgttct aatattccct ttttctgatt taaacatatt tgcttccttg
    30181 ggcacatagc gaatactaag ccttttggta taacttccaa atacatctat attccagtcc
    30241 tctttttgaa aatagcgaac tggaatacca gaatcatctt ccgagacaat ttctgtctta
    30301 gatagaattg attgaatgaa atttttatac ttttccatat gcatgagata gattgctgat
    30361 ttcatgatga ggtttaattt tttttgtttg gaaaaaaact ttccctcaac acttgtaggt
    30421 tccattgaat tcgggcttac aaaaaatttt aaaaaaatta attctctaaa cctaggattg
    30481 tcttttgagt gaaatatgat tcgaacccct tgataggcat ctggaacatc ctttacttct
    30541 tcgcttttga tttcttttat aattccatca ggggtaatgg aaattctttg tagttcatcg
    30601 atttgaaaac caattctttt taaaaaaact aacaagacag gtgccactcc agggaaatat
    30661 ttatttgcaa atttcttttc cattaaaata gttgtgaagt agttttgagt agaaatttgg
    30721 tttaggctag catctaacgt ataaaaagca ttttttaatt cttctggttt aaaaagaatc
    30781 ggatcattta tatagcccgg agtttctagt ccaaccatta tatatctatt tgattttgga
    30841 tagaaatgat ataggtttat aaaatcagca ccactgagta aatagagaga agtattttct
    30901 ttatgtgagt ctggaataaa ttcttttgac cattctgtta catgattaat atagttactg
    30961 gtaatctttc cccaagcctt atctattgat ctcgaataat ttttataata gttgagactg
    31021 gtaagaggta aaaagcgatt attctcatct agaggaaccc ctgcaaggta agacgccact
    31081 tcatcccaat agcttttatc tgcttcgttt ggttttattt ctaattcatc ccctgatgca
    31141 caaaaggaga ataataacgt gaatagaaat aggattgaga ttttccctaa aaattgaatt
    31201 gcctgatgaa gtttcaaaag taattggtcc atgctttgta aattttaaaa tagttgaaaa
    31261 gactaacgtt ttcgtgatct caatgttaac ttgtcttatg aatgtttaac aaattgtgat
    31321 ttgatttttt gcactgtgtc tatgttgtaa taaatttatt gctaatttta ttttttttca
    31381 tagattgtaa taattccatt tttgggaatt ccagtcgttc tcacaagttc aaagtcatgt
    31441 aacgttaata cttccaaacg agtatttttt ttacgcaatt cagacagttt gtcaataaat
    31501 attttattat tcgaaagatt ataagccgct tttgcgctga gtccaattcc ttctaagcat
    31561 tcttttgcaa gatagcattc atctcctgta attacaaatt ctttattttc cgttttgaga
    31621 tgcacgtatt gagatccaat tgtatgtccg cctgagaaat aaatggatat ttcaggagtt
    31681 aaataaaaat tactgtctat cttttgaatt tgatttctaa aatttcttac ttcaaatact
    31741 ttttcaaatt gtttaaatcc gtccgagctt tgaaatactt ccaactcttt tgtatttata
    31801 taaatctttg catttggaaa ataatgggct ccgccaatat gatcaaaatg gctgtgcgta
    31861 aggataatat ctgttacttg ttcaggagtt aaatttaaat ttcgaatgag caaggttgga
    31921 tgcttaaaat cagagatttg aaaagaggaa acatattttt tttcagtaaa gcctgtatct
    31981 accagtataa ttcgtctatc gacttgaatt agataaaaca gccatagaat cgtttttgtt
    32041 ttggtttcat cttttacatt taaaagttcg gatggatagg tgctttttcc atagacaagg
    32101 gcataaatac
//
LOCUS       DAOQOS010000059        31741 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_627_length_31741_cov_6.627501, whole genome shotgun sequence.
ACCESSION   DAOQOS010000059 DAOQOS010000000
VERSION     DAOQOS010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 31741)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31741)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31741
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_627_length_31741_cov_6.627501"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(362..1315)
                     /locus_tag="PLF66_13885"
     CDS             complement(362..1315)
                     /locus_tag="PLF66_13885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010572249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HMZ65183.1"
                     /translation="MNIQELREWISEAEKGEKYPLAKLISSIERKDSFSFRKTLFQEL
                     SQHPELKRNSITIGITGTPGAGKSSLIGELCHDFLKKQRRKKLAVVAIDPSSKLSGGS
                     ILGDRTRVVIPARENRIFFRSQASQLELGGVNPYTYHVIRLLRYFFDYIIIETVGIGQ
                     NETEVSRLADYSFLILQPLAGDGIQFMKSGIMEVPDAFIINKCDEKELATSSFHMLIS
                     SLEFLKDTLNGNTIPRIFQTSALKKIGIDSLLEFMLNIQEQKSKEMETKEQIEKMIRS
                     EYGNFGVRVLQEVSSTQSQFFMRQYETIEEDFNQEITKRIR"
     gene            1429..2649
                     /locus_tag="PLF66_13890"
     CDS             1429..2649
                     /locus_tag="PLF66_13890"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HMZ65184.1"
                     /translation="MNHLLTSNKIPKLTLALIFFTMLPVTMIVPVFKDLVKDRLGGDN
                     LMVSLFMSYAMLGSFLFSPVAGFLSDKFKNRKYFISVFAILDGICFFLLVLADNLQLL
                     QIIRFLEGVCHIFVIGLLLSLIADRENDSTNEHFYKKGILMGLAGMFLSLGVGIGSPM
                     GVLGKKNPLLPFYVAGTIMICIGIISFLLLHDYEFIYHERITFQKWSDAFQKNRLILI
                     PYLYNFIDRFTVGFFVTSFNLHLREELQFSAGEVGLYLSLVLIPMSLFSYPFARIAKK
                     TGPFLLMMVGSLLYGISLGISGGVNDKVFLFTLLILCGTGAGVMFVPSMMLASQMSPK
                     GMNASVMAGFTGVGSIGFMLGPIASTLMERFLKTNFESDFSFSILSLLFGSLEVLVVL
                     FTFPFRQKLKEAIQ"
     gene            2646..2822
                     /locus_tag="PLF66_13895"
     CDS             2646..2822
                     /locus_tag="PLF66_13895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65185.1"
                     /translation="MNLIWQNYFVYLIVLIAFIKLTQPIWEFLYRIAFAKKEVLVEDN
                     SCYTGTCAKCKVRS"
     gene            2819..3442
                     /locus_tag="PLF66_13900"
     CDS             2819..3442
                     /locus_tag="PLF66_13900"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012476717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HMZ65186.1"
                     /translation="MSFNQKYWDEMYAEEEGSVIDGIHNAKEHALYAKSILDLGEVKV
                     KSMADLGFGKAILLKEFTKQFRPERIIAIDPSEEAVSILKKQKWIQSTRHKIYKSTLE
                     KFNPKTKVDLGICNSVFQYISTPNIRKTYEKLAGFCKYLYFSVPTKEDYLYMEKELNF
                     VDPYALVRTKKFYLDSLSPYFTIVSHNLLESKILINESGFLYEFFRF"
     gene            3495..4541
                     /locus_tag="PLF66_13905"
     CDS             3495..4541
                     /locus_tag="PLF66_13905"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HMZ65187.1"
                     /translation="MKVTALGVHSAFATGTYKDVIETAKVEAMISEAVKNAKDTHAHL
                     TVNAIKNIIKQNTTRAYFPRYQSNFLLEFQTQGKIKQDVYRLVLDFGSDIRHSLANVG
                     LRMADIDGYYCSHPHADHIGGIEGIALSTVFNPFWNKKKTEWLSAEKKRRSDDDSPKQ
                     LDPITDRLFRREKIPADCKPDLWGHREVLDDLWDAARPGLDTLQGVKNVSLETFFNQI
                     VMSKSLEYQINDGEKSWIFYTVESTHVIGGTSYMPSYGLMFESSDKKIYFPTDTLYIM
                     PQIMKPFYESADIIYHDCETGPRSGVHSHIDDIRKIDPAIKKKCYLYHYTEEPQVDPD
                     EFRGIMHTGDVHEY"
     gene            5035..5589
                     /locus_tag="PLF66_13910"
     CDS             5035..5589
                     /locus_tag="PLF66_13910"
                     /inference="COORDINATES: protein motif:HMM:NF039392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6266 family protein"
                     /protein_id="HMZ65188.1"
                     /translation="MAKIINTFGPIANGKIGDLVFYQLKGKNYVRSAQNRFKDHKTQK
                     QIEHRNKFATLIQLARLFKSVVRFQVKNTNTNFQTFCKHNWKNISIQYGKVVVDYDNL
                     ILTDHFPLPLLHLEVKKQSNQIFFQWAKDHLNLDDSFKTICVVFSKEQEKVFFTEVNR
                     NDLSALVTLPYDVYSYHTFTFTYK"
     gene            complement(5842..5979)
                     /locus_tag="PLF66_13915"
     CDS             complement(5842..5979)
                     /locus_tag="PLF66_13915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65189.1"
                     /translation="MEEVSSIDLIIEKYKEGIDETLLIENLKLTYTERRLSKITTDRH
                     G"
     gene            complement(5983..8247)
                     /locus_tag="PLF66_13920"
     CDS             complement(5983..8247)
                     /locus_tag="PLF66_13920"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004428736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HMZ65190.1"
                     /translation="MAFDIEMIKNLYSKMGARIDAARKIVGRPLTLSEKILYSHLWDG
                     EPKTVFERGKSYVDFAPDRVAMQDATAQMALLQFMQAGRKKVAVPSTVHCDHLIEAKV
                     GSDADLSIANEKNREVYDFLKSVSNKYGIGFWKPGAGIIHQVVLENYAFPGGMMIGTD
                     SHTVNAGGLGMIAIGVGGADACDVMSGLPWELKFPKLIGVKLTGKLNGWTSPKDVILK
                     VAGILTVKGGTGAIVEYFGEGAISMSCTGKGTIANMGAEIGATTSTFGYDDSMARYLK
                     STGRADVAALADGIKQHLTADPEVYADPAKYFDQLIEINLSELEPHLNGPFTPDLATP
                     ISKLKEAALANGWPTKIEVGLIGSCTNSSYEDISRSVSLAKQAAEKNLKPKAEFTITP
                     GSEQVRYTIERDGFIEVFNKIGAKVFANACGPCIGQWARAGAEKAEKNTIVHSFNRNF
                     AKRADGNPNTYAFVGSPELVTAFAIAGDLTFNPITDYLTNEKGEKVKLDPPVGEELPA
                     KGFAVKDAGYQEPVADGSNVQVAVSPTSDRLQLLDSFPVWEGTDLKGLKLLIKAKGKC
                     TTDHISMAGPWLKYRGHLDNISNNMLIGAVNFFNEKTDSVKNQLTGSYDSVPKVQRAY
                     KAAKVGSIVVGDENYGEGSSREHAAMEPRFLGVRAILVKSFARIHETNLKKQGMLALT
                     FANKDDYAKIQEDDSIDILGLTTFKEGVPLTLVLNHKDGSKEEIKANHTYNASQIEWF
                     KAGSALNIIKAQQK"
     gene            complement(8594..8827)
                     /locus_tag="PLF66_13925"
     CDS             complement(8594..8827)
                     /locus_tag="PLF66_13925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65191.1"
                     /translation="MDAYNSIPKQSKKEKEIYLETLRRMTPTEKFNKVMELNELTKSL
                     FKQGLRKRFPEKTEEEIHILFLKRLEKCHNSNY"
     gene            complement(8836..9216)
                     /locus_tag="PLF66_13930"
     CDS             complement(8836..9216)
                     /locus_tag="PLF66_13930"
                     /inference="COORDINATES: protein motif:HMM:NF020903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1987 domain-containing protein"
                     /protein_id="HMZ65192.1"
                     /translation="MENLKLEKTKTTPKVDFDAKLNTLSITGEVYPEDAEKFFKPIFE
                     WLNRYLAQLHHKCIVSFNLKYFNTASSGRISQFLETLEKAYKKGKDINIHWYYEPEND
                     VLEEVAEEFREIVTIPFQIIEKKL"
     gene            complement(9260..9682)
                     /locus_tag="PLF66_13935"
     CDS             complement(9260..9682)
                     /locus_tag="PLF66_13935"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HMZ65193.1"
                     /translation="MEIIVEKTQDPNESAFLWARLREHGLEKLSNPSLEYNCLFSILV
                     KEAEEIIGGMLGEIYYKGLHISLLWVREDYRNKGVGTILMEKAEGLARETESTVVYLD
                     TFSFQAPRFYEKLGFSILGQIENFPEGYTRFFLFKRIL"
     gene            complement(9975..10472)
                     /gene="tpx"
                     /locus_tag="PLF66_13940"
     CDS             complement(9975..10472)
                     /gene="tpx"
                     /locus_tag="PLF66_13940"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HMZ65194.1"
                     /translation="MADITLKGNTIHTVGNLPKIGEDAPSFTLTKTDMSDVTNKDFAG
                     KKVVLNIFPSIDTPVCATSVRKFNAEATKFPNTVVLCVSTDMPFALNRFCGAEGLKDV
                     IPVSELRKREFGEKYGVRIADGPLAGVFSRAVVIIDENGKVVYTEQVPEIAQEPNYAK
                     ALEKL"
     gene            complement(10840..11496)
                     /locus_tag="PLF66_13945"
     CDS             complement(10840..11496)
                     /locus_tag="PLF66_13945"
                     /inference="COORDINATES: protein motif:HMM:NF013586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HMZ65195.1"
                     /translation="MIILPFLVIFFTQTLAADDRVGEALSKSPDFIWIQNSESIQIDL
                     KYASANNFMKENLYGSYNKCYLHREANEKLKRAIQKLNEKKPKFKFVLFDCLRPRSIQ
                     YKLWDKVKGTSQEPYVANPKSGSIHNYGFALDLSILDETGKELDMGTPFDSFLPLSEP
                     TKEEAFLKSGELTKKQWENRILLRNIMQDAGFIQRPNEWWHYDGFSAKTIRKNYKIIE
                     "
     gene            complement(11634..12242)
                     /locus_tag="PLF66_13950"
     CDS             complement(11634..12242)
                     /locus_tag="PLF66_13950"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HMZ65196.1"
                     /translation="MKIGIICGSHRKDSESAKVSSYFETLIKADQKDIYLLKLGTSGI
                     PLWEESAWTDGEVWKEKWQPHSKELKTCDGFLIVSPEYGGMAAPAIKNFFLLCNKQEL
                     SHKPGLIVTVSGARGGAYPVSELRASSYKNTQICYIPEHIIVRNAKQVLNDNPENPLE
                     EDLYLRKRIPYALKLLYSYTDALQLVRNSSIPDYRAFGNGMS"
     gene            complement(12293..12934)
                     /locus_tag="PLF66_13955"
     CDS             complement(12293..12934)
                     /locus_tag="PLF66_13955"
                     /inference="COORDINATES: protein motif:HMM:NF019238.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1569 domain-containing protein"
                     /protein_id="HMZ65197.1"
                     /translation="MTAIFIKRYTFTNLDQVLDHLAILEKSNYIETTGLWSFYQILTH
                     CKDHIHYSMNGFPYYYAAFFRKTLGKYLLSKLLKRGYMEPNGYNGDTISNRVEGNEMQ
                     ALNELREAIYAFRSFNGEFASHPMYDKMDKATWEKFHSMHIANHLSFVEYLPEKMRMK
                     DSVSENLGKEEPISKPSIPVTTLSKLEAMDAMIRKPVPKSKGKIKNVIQKRTK"
     gene            complement(13562..14263)
                     /locus_tag="PLF66_13960"
     CDS             complement(13562..14263)
                     /locus_tag="PLF66_13960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002556565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HMZ65198.1"
                     /translation="MSKKNDFSTLDLISAYEGKPKTTQTLSLKDIEPSPNQPRQFGKN
                     NVDDLVDSMKRMGLIEPIVVRKNNSKYLIVAGERRFHAAERLGWKEIPAIVTEANPEL
                     CYEMALAENEKRKSLNPWEVGKAIQYLRKERKKTALEVADILGYTERYVKQLSSIARL
                     ELKHVSEFIGQGNDPSVKNLEKLLKQKEGRGGEMISSSKKDKTSISLKKLNQKQRESF
                     LKEIRPIAKKYGIEI"
     gene            complement(14247..15176)
                     /locus_tag="PLF66_13965"
     CDS             complement(14247..15176)
                     /locus_tag="PLF66_13965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015679370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HMZ65199.1"
                     /translation="MKKKEKVDLSIEEASEFYGVSVIGLQKKIKEWKIPVSKSGKYQT
                     EILDKYFTRNQSVDKVANVIAISNQKGGEGKTTISLFLSEALSFDSKVLLVDWDPQAN
                     ATRLFVNEPERTVFDCLGYRKSQKFSVDTVIIPIRPNFHLLPSSLSLANLTTPFERDD
                     FELLKDALLPVLSSYDFILIDCPPSLGLGLENALIAADYIIVPVQTRAFSVQGLKDLH
                     ETIQKIQNKANPRLKLLGAVFNQFEGNRALSGLSESVKKYFPVFKTSIYRKESIPQAQ
                     AKRKMLDGCDKETNKSFLDLAKEVRSKIHVKEK"
     gene            15325..16323
                     /locus_tag="PLF66_13970"
     CDS             15325..16323
                     /locus_tag="PLF66_13970"
                     /inference="COORDINATES: protein motif:HMM:NF028249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative porin"
                     /protein_id="HMZ65200.1"
                     /translation="MKPYSILFIGMSFFIPLLQIFGDGGFVGIIGARNEGEHIFETGN
                     KYPNLSGIRGGSRITYQRNFNLAGIEGGYFKNKYSFRGRVISNFWNQNSGGTRDEDFF
                     LGNSSSEKGSKFSPFQGVLHDTAYTFTGTANFADGKGRSTISEYNLEGFFRYHFGDAS
                     PDPWATGSGFFLSGGLRYSYAKLQVYDVIQYVASRPIFYGPIGSGLSYSYSFTEIPVG
                     GGYIFNLGNFKIEPSVHLLYAFIKTRDFHIQRALNFLAHTAGPGFLARIEGSYSISEN
                     AMIKIGVTGHRQFTGGSFKTRGGLYENDILSNYLGSYKSYINTKEVIFDMSYIMRL"
     regulatory      16360..16454
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            16497..18113
                     /gene="thiC"
                     /locus_tag="PLF66_13975"
     CDS             16497..18113
                     /gene="thiC"
                     /locus_tag="PLF66_13975"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016545285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HMZ65201.1"
                     /translation="MNNPNQFQSLAKDFPLKEKIQLDGHEIPEKKIKLTNGQEVCLYN
                     TEGPIGFDPKKGIPKRRIEWVKERKSRGDQNFSQMHYARKGIITEEMKFVAFREGIDP
                     EFIRSEIALGRAIIPANINHPELEPMIIGRNFLVKINANIGNSAITSSIEEEVEKLKW
                     AIKWGADTVMDLSTGKNIHETREWIIRNSPVPIGTVPIYQALEKVHGKAENLNIDLFI
                     ETLIEQAEQGVDYFTIHAGVLLKYIPLTAKRITGIVSRGGSIMAKWCLAHHKENFLYT
                     HFERICEVMKKYDVSFSLGDGLRPGCTADANDEAQFGELQTLGELTKIAWKHDIQVMI
                     EGPGHVPMHLIQENMQKQLEICHEAPFYTLGPLVTDIAPGYDHITSGIGAAMIGWMGT
                     AMLCYVTPKEHLGLPNKEDVKTGVITYKIAAHAADLAKGHPGARNRDDLLSKARFEFR
                     WEDQFNLSLDPETARNFHDETLPQERMKEAHFCSMCGPHFCSMKLTQDVRDFAEKNGY
                     SQEEVFAKGMEEKSNEFKEKGSHLYTVSQS"
     gene            18209..19288
                     /gene="hemH"
                     /locus_tag="PLF66_13980"
     CDS             18209..19288
                     /gene="hemH"
                     /locus_tag="PLF66_13980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011671250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HMZ65202.1"
                     /translation="MKVTLVNLGGPRTSDEIEQFLLDLFCDPLVFDLPIPEFFRIRLA
                     RFIAKKRAPKVAHAYASMGFGGGSPLVAETEKQAKALEKKLIEKTKQEWKVQVAMACG
                     YPNLRELPKEDLIPSKDNIILPLFPQFSRSTTMSIAKLIESQTNQCPANLQNGSCGNH
                     CGTNKKGAICPLNRKGFVAPFHKDPKFIEATGKLILDYFQGKLNAKDFLHLDTAKGIE
                     NWQEITIVFSAHGIPIRLIEKGDIYRTEIEENVKNITAELNRFGFRGKSFLSFQSRVG
                     PTKWTEPNTIRMLEELGKNGHKKVAIFPISFVSDHLETLEEIGVQLKEVANASGITDY
                     FRIPAHGIYPDFIEALASIVLKERD"
     gene            19848..22418
                     /locus_tag="PLF66_13985"
     CDS             19848..22418
                     /locus_tag="PLF66_13985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016547526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HMZ65203.1"
                     /translation="MSIFSSIESYSRNVKIRTKMIILISGVVLISVLPLSLIVLYRNQ
                     AVVLNKTFEVCSNLAQNISNLATEELLMNETYDTTRTSIKRLKDSNISGLLESYVVNV
                     DGKYVADLNEQRIDQQISDSDKGYFKKLEKLEMKEINSNSNQPILRFSYPIFITEYET
                     KEIRKVGIAVFEFDKKKVYEPVVQIRTTIIGVAGVLFIVGIIIAAYAAFAFSKPIQKL
                     SEGAQKIGGGDLNHRIKLTGSDELGQLAKSFNLMTSQIQDFTQNLELKVAQRTEELNR
                     TLQEVQALKIAQDGDYYLTSILLNPLQPNNNRSSKVKTEFMIEQKKKFSFRKWHSQIG
                     GDICITDTITLNGKEYTVFINGDAMGKSIQGAGGALVLGVVFNAGLIRSRIEKFQSTY
                     PEIWLKERFLDLHNVFLSFDGSMYISICLGLIDNSTGLLYYINAEHPWTVLYRDGKAS
                     FLEQELALRKIGTPDQEDQFYVRMFQLMPGDVVITGSDGRDDLVLKNKEGIEYIQEDE
                     SQFLLRVEEGEGKIAQIAQRVKETGALMDDFSLLRISFNEEYAELENTKEIPYHVTES
                     VSAGMELLEEGNAEEAIKKVEQFLHDYNDFPDMLKLLGKVYFQKGDFPKAIECFEQYL
                     TLNPSDNEYLYALSNTYRVYGKLNLAADIAERLFLRDQKHLLNLINLATIYYGLRVFG
                     RAESMIKRALYIDPENKHAELLYESIREAIIEKSSNNNGQVKKLTLSERLKAENENLL
                     EQAEELYKNKDYQGALILYQQLLDDENSENSRILLKIANCNSFLNKLDQAIIFYDRAL
                     KTDPMNYHAHNNLGGIYFKQGLYLKAKNQWMKSLEIKTDFKPAEINLQRLEKMESKLS
                     "
     gene            22443..23963
                     /locus_tag="PLF66_13990"
     CDS             22443..23963
                     /locus_tag="PLF66_13990"
                     /inference="COORDINATES: protein motif:HMM:NF016292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HMZ65204.1"
                     /translation="MVRKIKLILTLSLFLTHAIYADKPIEVLLSSDNRIYEQGLYGIQ
                     SVFEGELKISYLDIITAEQPDIASYFKAIDDSGAPLFITIGPAATKAAKENLKKTPIV
                     FSMINSPKSLGIDGGNLCGVSMDISIGEFFQALKDIKPSAKTVTAFYTTNDGEYSAGE
                     GEYTDLKYKLIYNRKKLADKKEFKTALEEIKGKTDAFFMVNDPLYSSVEFEILSEFAK
                     KNSIILMTSFPALVKVGATFGISPDYSKIGVLTGQMANRINSKSSTCSEERVLLPDQS
                     SFFLNEKYAKDSGIDLPEAIVERAKLTKLFDVGVNLFNEGKLNSAKIVFEAILKRDPG
                     NKSAYSFQQLILERMSGTKTRELLASADNHFKNKNYAQARADYQKVLAINPSIGAAKE
                     GIQASLLAQSEQERAQGTDLAKRGKPFEAIKMFMAALRTLPSNAQANADLNAIRNFES
                     PNMKSYIEKGIAHYNDRNYNSSIEIFENALLVVPNNKEAIEYLRLSYKKRDAMIVL"
     gene            24764..27664
                     /locus_tag="PLF66_13995"
     CDS             24764..27664
                     /locus_tag="PLF66_13995"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HMZ65205.1"
                     /translation="MRFFALISIVFLTTSLFAQENQNKSKIIYITHFNAYKSQKSDSI
                     SNKIYKSLEEDFKSLGYEVRESNTDLSSTLKTAKANNSMFVIDGYYKLNETGGLSIYS
                     QIYNPDTGYMIDALNITDELYGAEGVSLDPKETQKPADSTIDEFKKKVVIRIRTNTKR
                     TERRENINEAVTTTALGKDKDLKFPIAEENVASASADVFKILAEKETVSVASNVIKDA
                     KKQPVSVSIITKEQIKMSGARTVNEVLTTYVPGFFTVEDQDDTIAGFRGFAPDSNAKV
                     LLLINGHNMNTEWFWGPPDSIINGLNMEYIERIEVIRGPGSVTLGQGALLGVINIITK
                     NGSTTNGTTLSTKLGQNNYSIGTLQAGGSGKENPDLKTFFQVSTARYNGQELRSEGWA
                     RTQTLSGQEGYYDYQRGLTQLVPTADVSKKYSDSLRLFDTYTPDPSGGFNSVITRRNV
                     ATSGARLKRADSDVVTGVINYKNLELTGFYTNQTRDLYNFYRDRNRVQNTIKSGTITY
                     TYDVSDKVSLKFKNYYTVDDIFLRSQKGLTLGGTREYRYGGSIILGLNDLIKNNNTAI
                     GIEFRKYDMGQVNSDGNNFILNYSQNTADNALLLDKNGLTPNERNRYVYPGSIAVRSF
                     FMEDFYKLSEKIDIFGAFRYDKHPYWGSNVAPRVGALYEANKDLRFRFSYQEGFRGVV
                     GVSYAGGFEGDGHLRIQNFPYIEASNIPSSFDKQGYPSGFYRDVPKTKPEKMKSFEFA
                     TNYSFTSNLSLENILFFNKVEKVIDVGVLYCDKPNPTATAPGPNGCNMPRLGNDTPGN
                     WNGYWFYKNNPGEIRQGGAEISLNYKNRLISATFSQSIVKLLTASPGQTDSIYLTSDP
                     NNRQFRGYPSNVTRLHALLYPVDKVTVSFTYIFYPSWYSPTNERVEGNHLANLGFNYK
                     YLENFEFHFILKNLFAAGNLYPMISNAGGPDLSNGTPAVEKRTFWGGFSYTF"
     gene            complement(27661..28026)
                     /locus_tag="PLF66_14000"
     CDS             complement(27661..28026)
                     /locus_tag="PLF66_14000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65206.1"
                     /translation="MIFKNKYILFLMLIAQSCTGVYYGIDGPDVISRHDAVNKINDAY
                     LVKFASCGFDSTNVALLVKRLTTSDRKILDGAYYATKDVDSCVKQVLFISCKDSAAFT
                     CNISPKDFFGGGGLLQGGF"
     gene            28890..>31741
                     /locus_tag="PLF66_14005"
     CDS             28890..>31741
                     /locus_tag="PLF66_14005"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HMZ65207.1"
                     /translation="MKLHIQTFFLATFFLIAIPVYSQDKQNKKIFISTFSAYKSNKDS
                     NVSNKVVESLTSKLQSVGYEVSNIESENLKDNLNQSKQKGAGLLISGFYRREDNGNLS
                     LYGQIYNPDKEVMIDALNLTTDISGIEGIALDPKEIKTSDEQNIDDFSTKITNRIKAN
                     IKRSERHENINENIVNHPIGKENTFPLPKEDLNATSEEVFKLLSEKDDVVVSVSKFSQ
                     KTSEAPADITVISREQIRRSGFRNLTEALNFVPQVYTHWIGQNWSSDFRGLFVNNQIE
                     RRVLYLQDGKKLNDYFHFGEFYSDVYTDMERIEKIEVIKGPGAALYGNNSITGVVNVI
                     TRKPTKKNEMELVTEYDSVLRNLTTRALYYSKFSEKFSVSLDASRFEGKGYYTSGYNS
                     WGGTRYYDPNSAANAPNFNPTPANSTFGTSEYGRNTAEVETGQRAWTSTGLMANNGKW
                     FPNYNLDVKYGDWNLRSFYMSKRTSWIPPQSDSGAFGGDTVYGSPRNDRIWGVGVVSL
                     DYTPSFLEKYEASFKIFRQINLNSDYREKDYQGFAQATNPPGVPSSYGSTPATRLTSQ
                     TYLNYFQSMGGGVVKRYSSTANTQGIEYQVTPYKIDNKDAIIKSFRFMTGGNMQAVNY
                     INYQTNVGLNGLIDRRQQGIADDGRQFGIWTQLTSTFKTDTTIVLGLRYDAQKIYNVY
                     RHQNGMLNDVAQESVTDPYLRTPSTGGNQPFVGPGNTINLTTPVNDVFGNRVATGYVQ
                     PFQRKNFVAEDKTPRIAFIQNIKATDTTIKLMYAEAFRMVTPQELIRLPRELGNAQSE
                     KVRNREINIIQPLFKGALVLNFDYFRMTGSTIYAFNAATLAFGQSPEWSNNGGSLSVT
                     YVVDNKWRLNGSYSSYQLRRPSDSSFLNTLFTPKSQALNSPTKLWKAAISRSVINDNY
                     TISLEFYYNGSIYLMENPPRTNSQVLNNDGTLRELPP"
BASE COUNT        10681 a         5599 c         5416 g        10045 t
ORIGIN      
        1 gtaggctcca ctaattcttg ctttttacaa ttcagaaaga aaactaaaac aatcaaaaat
       61 atagcgttaa acgctatcca tatcttattc ataaaatctc cttgggttcc ccgattcatc
      121 taaaaatctg gtctcatctg aactgaaaca ttccgattcg tcaagaatat aaaaagttaa
      181 aaacaatttc tagtttgaat atacctctaa atgttgactc aatgaaattt tagaagggaa
      241 agtaaaagtc gcttccgaat ctacttttcg aataatagta ttggaaattg atcaagaaat
      301 aggattaaat ctaaaaaaaa ttcgctatgt gatataagaa atatatcgta ttcgtgtgaa
      361 tttatctaat tctcttagta atttcctgat tgaagtcttc ttcgatagtt tcatactgac
      421 gcataaaaaa ttgagattga gtagaagata cttcttgtag aacacgaact ccgaaattac
      481 catactcaga gcgaatcatt ttttctattt gttctttggt ttccatttct ttagattttt
      541 gctcttgaat atttaacata aattccagaa gagaatcgat acctattttt ttaagagcac
      601 tggtttgaaa gatacgagga attgtatttc cgtttaaagt atctttcaaa aattcaagag
      661 aactaatcag catatgaaag ctagatgttg ccaattcttt ttcatcgcat ttattaatga
      721 taaacgcatc tggcacttcc atgattccac ttttcataaa ctgaattccg tcgccggcta
      781 acggttgtaa aatgagaaaa gaataatcag caagtcttga aacttctgtt tcattttgtc
      841 caattccaac cgtttcgata atgatataat caaaaaaata acgaagcaat cgaattacat
      901 gataggtata aggatttact cctcctagtt ctaattggct cgcctgtgat cgaaagaaaa
      961 tgcgattctc acgggctgga attacaacac gcgtgcggtc tcctaaaata gaaccaccac
     1021 ttaacttact agatggatca atcgcaacga ctgctagttt cttgcgacgc tgttttttta
     1081 aaaaatcgtg gcataactct ccgatgagag aagatttccc cgctccggga gttcctgtga
     1141 ttccaatggt aattgaattt cttttgagtt caggatgttg tgacaactct tggaataaag
     1201 tctttcgaaa agaaaaagag tcttttcgtt ctatagagga aattaatttt gcaagtggat
     1261 acttttcccc tttctctgcc tcactaatcc attctcgtaa ttcttgaatg ttcataaaat
     1321 tcttgctgaa gtctctaact gattttacaa cgttaaaaag gtcaaagtat tttaaaacaa
     1381 tttttatctt atgtggaaat tgaatgcaaa ctgtttaaac aatcatctat gaaccattta
     1441 cttacatcaa ataaaatccc aaaactaaca ctcgcgctta ttttttttac aatgttaccc
     1501 gttacaatga ttgttccggt gtttaaagat ctagtcaaag atcgactcgg gggagacaat
     1561 ctaatggttt ctctttttat gagctatgcc atgctcggtt cttttttatt ttcacctgtt
     1621 gccggctttc tttcagataa atttaaaaat agaaagtatt tcatttcggt atttgcaatt
     1681 ctagatggaa tttgtttttt tctacttgtg ctcgctgaca atttgcagct actacagatt
     1741 atccgcttct tagagggagt ctgccatata tttgtgattg gactccttct ctctttaatt
     1801 gcagataggg aaaatgattc tacaaacgag catttttaca aaaaaggaat cctaatggga
     1861 cttgcaggaa tgtttctcag tttaggagtg ggaattggtt ctcctatggg agtgctcgga
     1921 aaaaagaatc cacttcttcc tttttatgta gcgggaacaa ttatgatttg cattggaatc
     1981 atttcctttt tactcctcca tgattatgaa tttatttatc atgagcgaat aacgtttcaa
     2041 aaatggtcag atgcatttca gaagaataga ttgattttaa ttccttatct atataatttt
     2101 attgatcgct ttactgttgg tttttttgta actagtttca acctacactt aagggaagaa
     2161 ttgcagttta gtgcaggaga agttggtctt tatctttcac ttgtcttaat tcctatgagt
     2221 ttgttttcct atccattcgc aaggatcgca aaaaaaacag gaccctttct tttaatgatg
     2281 gtaggctccc tactctatgg aatctcactt ggaatttctg gtggagtaaa tgacaaagta
     2341 ttcttattca ctctactcat cctttgtgga acaggggcag gcgtaatgtt tgttccctct
     2401 atgatgcttg cttctcaaat gtcaccaaag ggaatgaatg ccagtgtgat ggcaggattt
     2461 accggtgtag gatcgattgg ttttatgcta ggtcctatcg cttctactct catggaaaga
     2521 tttttaaaaa caaattttga atctgatttt tctttttcga ttctttctct tctctttggt
     2581 agtttagaag ttttagttgt tctatttaca tttccatttc gtcaaaaatt aaaggaggct
     2641 attcagtgaa tctgatttgg caaaactatt tcgtttatct cattgtattg attgcattta
     2701 taaaattaac ccaacctatt tgggagttcc tctatcgaat cgcattcgct aaaaaagaag
     2761 tattagtcga agataattct tgttatacag gaacatgcgc caagtgtaag gtaaggtctt
     2821 gagcttcaac cagaagtatt gggatgaaat gtatgcggag gaggaaggtt ctgtcattga
     2881 tggaattcac aatgcaaaag aacatgctct ttacgcaaaa agcattctag acttgggaga
     2941 agtaaaagtc aaaagcatgg cggacttagg tttcggaaaa gcaattctat tgaaagaatt
     3001 tacaaagcaa tttcgaccgg agcggataat cgccattgac ccatcagaag aagctgtttc
     3061 tattttaaaa aaacaaaaat ggatccagtc tacacgacat aaaatttaca aatctacatt
     3121 agagaaattc aaccccaaaa cgaaagtcga tttaggaatt tgtaactcag tatttcaata
     3181 tatctccaca cctaatataa gaaagaccta cgaaaagctg gctggatttt gcaaatatct
     3241 atacttttct gttccaacca aagaagacta tctctacatg gaaaaagaat tgaactttgt
     3301 tgatccctat gcattggttc gaactaaaaa gttttattta gactcactat ctccctactt
     3361 cacgattgtc tcgcacaact tactcgaaag taaaattcta atcaacgagt ctggttttct
     3421 ttatgaattt tttcgttttt aaataaatga gtggcaaaaa ttttcgatac attgaaatct
     3481 gttaagggaa gattatgaaa gtaacagcat taggcgttca ttcggcattc gcaacaggca
     3541 catataaaga cgttattgaa actgcaaaag tagaagcaat gatttcagaa gcagtcaaaa
     3601 atgcaaaaga tactcacgca cacctaacag tcaatgcgat taaaaacata atcaagcaga
     3661 acaccacaag agcctatttc ccaagatacc aaagtaactt tctattggaa tttcaaacac
     3721 aaggcaagat caaacaagat gtttacagac ttgtattaga ttttggaagc gatattcgac
     3781 attccctagc aaatgtagga cttagaatgg ctgatataga tggatattat tgttctcatc
     3841 cacatgcaga tcatattggt ggaatcgaag ggattgcact ctcaaccgta tttaatcctt
     3901 tttggaataa gaaaaaaaca gaatggctct cagcagaaaa aaagcgcaga tctgatgatg
     3961 atagtcctaa acaacttgat ccaattactg acagactctt tagaagagaa aaaattccag
     4021 cagattgtaa gccagatctt tgggggcaca gagaagtttt agatgatctt tgggatgcag
     4081 cacgacctgg ccttgatact ctacagggtg taaaaaatgt ttccctcgaa actttcttta
     4141 atcagatcgt tatgtccaaa agccttgaat accaaatcaa tgacggcgaa aaaagttgga
     4201 ttttctacac agttgaatcc acccacgtta ttggcggaac ttcttatatg ccatcctatg
     4261 gtcttatgtt tgaaagcagt gataagaaaa tttacttccc aacagacact ctctacatca
     4321 tgccacaaat catgaaacca ttttacgaat ccgcagacat catctaccat gactgtgaaa
     4381 ccggtcctcg cagtggagtg cattcgcata tagacgatat acgaaagatc gatcctgcaa
     4441 ttaaaaagaa atgctatctc tatcactaca cagaagagcc tcaagtagat ccagatgaat
     4501 ttcgcggtat catgcacaca ggcgacgtgc atgagtattg attttaacca cagatagtca
     4561 tggatgaaga ctgatgggtt tccatattcc ttttctcaaa aaatattttt ctttttgatt
     4621 agacagttct catttaatta tatttttttc tgacaaattt ttaataccca ttctaaatga
     4681 tacgcgctac ccccaccctt tctatggact tggtatagtg gttcgtatag tgttgctata
     4741 gtccttgcta tatggaaaat gaattttgag tataggatat actctattag ccgctaagcc
     4801 atcgattaga aaaaggattt ctttatattc ccttcaagcc tatcgtttaa aatcgtttcg
     4861 ttataggtgt tagcgaagcg atcggtggta atcttttaaa aattttttgc gcaagtgcgt
     4921 aatgtttgaa aaaatatttt ttaagaagat aaagatatat ggttctaaag aacgataaac
     4981 gtatgaatgc aactaaatta agtggcattc aattttatat acaggtatta ttttatggct
     5041 aaaattataa atacatttgg acctattgca aatgggaaga taggtgattt agttttctat
     5101 caactaaagg gaaaaaatta tgtaagaagc gcacagaatc gctttaaaga ccataagaca
     5161 caaaaacaaa tagagcatag aaataaattt gcaacactca ttcaactggc tcgtcttttt
     5221 aaatcagtcg ttcgctttca ggtaaagaat acaaatacaa atttccagac cttttgcaaa
     5281 cacaattgga aaaatatttc gattcagtat ggcaaagttg ttgttgatta tgacaatctg
     5341 attttgacag atcattttcc tcttccccta ctccatctag aagtaaagaa acaatccaat
     5401 cagattttct ttcaatgggc aaaagaccat ctaaatcttg atgattcttt taaaacaatt
     5461 tgtgttgttt tttctaaaga acaggaaaaa gtattcttta cagaagtaaa tcgaaatgac
     5521 ctttccgcat tggtcacact cccgtatgac gtttattcgt atcatacttt tacgtttaca
     5581 tataaatagg agtagcgttt ggtataccga acatactgat gttcgggaac acaaataagt
     5641 gtttagaccg aacacttatt tgtaattgaa aaatgggaaa ttaattttgg cgttcggtat
     5701 aaatcggggt agttaaagtg aattcgatgt aaaaattttt gaccactgac ttgcactgag
     5761 cctgtcgaat gtgtgcacac cgataactga acgataaaga tgcgatagat aattttaaat
     5821 catcgtatct tttatcggtg ttcatccgtg tctatcggtg gtaatctttg aaagtcttct
     5881 ttctgtgtag gtaagtttta aattttcaat gagtaaagtc tcatctattc cctctttgta
     5941 tttttcaata atgaggtcta tagacgagac ttcttccatc ttttacttct gttgtgcttt
     6001 gatgatgttc agtgcagaac ctgctttgaa ccattcaatc tggcttgcat tgtaagtatg
     6061 gtttgccttg atttcttctt tcgatccgtc tttgtgattt agaactaacg tcaaaggaac
     6121 tccttctttg aaagttgtaa gacctaagat atcgatggaa tcatcttctt ggatctttgc
     6181 ataatcgtct ttatttgcaa aagtcagagc aagcattcct tgtttcttta aatttgtctc
     6241 atgaatacga gcaaatgatt ttacaagaat tgcacgaact ccgaggaaac gaggttccat
     6301 tgccgcatgt tctctagaag atccttctcc ataattctca tcccctacta caatcgaacc
     6361 tactttagct gccttgtaag ctctttgcac ttttggaaca gaatcataac ttcctgttaa
     6421 ttgatttttt acactgtctg ttttttcatt aaagaagttt acagcaccaa tcaacatatt
     6481 gttggaaata ttatctagat gtcctctgta tttgagccaa ggtcctgcca ttgagatgtg
     6541 atctgtagtg cactttcctt ttgctttgat taaaagcttt aaccctttta gatctgttcc
     6601 ttcccaaact ggaaaactat cgaggagttg caagcgatcc gatgtaggac ttactgctac
     6661 ttgaacgtta gacccatctg caactggttc ttggtatcct gcatctttta ctgcaaagcc
     6721 ttttgctgga agttcttctc ccactggagg atctagtttt actttctcgc ctttttcatt
     6781 tgttaaataa tctgtaattg ggttaaatgt taaatcccct gcaatcgcaa aagcagtaac
     6841 tagttctgga gatcctacaa aggcgtaagt gtttggattt ccatctgccc gttttgcaaa
     6901 gttacggtta aacgaatgca cgattgtgtt tttctctgcc ttctctgctc ccgcgcgcgc
     6961 ccactgtcca atacaaggtc cgcaagcatt tgcaaaaacc tttgctccga ttttattaaa
     7021 tacttcaata aaaccatctc gctcgatcgt atatcgcact tgctcagatc caggagtaat
     7081 tgtaaattct gcttttggtt ttagattttt ctctgctgct tgttttgcaa gagatacaga
     7141 tctagaaata tcttcataag aggagtttgt gcaagaaccg ataagcccta cttcgatctt
     7201 tgttggccaa ccatttgcca aagctgcttc ttttaattta gaaatcggtg tagctaaatc
     7261 aggagtaaat ggtccgttta aatgaggttc taactcagag aggttaatct cgattagctg
     7321 atcaaagtat tttgctggat ctgcatatac ttcagggtct gctgttagat gttgtttgat
     7381 tccatctgca agagctgcta catctgcacg accagtcgac ttgagataac gagccattga
     7441 atcatcatat ccaaatgtag aagtggttgc cccgatttcg gcacccatat ttgcgatagt
     7501 tcctttacca gtgcaagaca tagaaattgc accttctccg aaatattcta cgatagcacc
     7561 agttccacct tttactgtca gaattccggc aactttgaga ataacgtctt ttggggatgt
     7621 ccaaccattt agcttacctg ttagtttaac gccgattagc tttggaaatt ttaattccca
     7681 aggaagacca ctcatcacat cacaagcatc tgctccccct actccgatag cgatcattcc
     7741 gagtccgcca gcgtttactg tgtgagagtc ggttccgatc atcattccac ctgggaatgc
     7801 ataattttct aaaacaactt ggtgaataat accagcacct ggtttccaga atccgattcc
     7861 atatttattg gaaactgatt ttaagaagtc atagacttct ctgttttttt catttgcgat
     7921 agaaaggtca gcatcggatc caacttttgc ctcaattaaa tgatcgcaat gaacggtaga
     7981 cggcacagcc actttctttc taccagcttg catgaattgt aataaagcca tttgtgcagt
     8041 agcgtcttgc attgctactc tatctggagc aaagtctaca taagatttgc cacgttcgaa
     8101 tactgtttta ggttccccat cccagagatg agagtagaga attttttcag agagagtgag
     8161 tggtctgcct actatttttc tagcagcatc aattcttgct cccatcttcg aatataagtt
     8221 ttttatcatt tctatatcaa atgccattgt tcccgttcct aaatttatat tgatttaatt
     8281 tatatactca ctctacgcaa aattaataaa ttgagccaca aaagatgatt ttgaaaatat
     8341 tagagacagt tattgcacta atacaatcaa tagcctgcct tatgtttcga cccttaggga
     8401 gaaacatgag tttcgcaagc cctccgctag gagtaaatct catttgaacg tttggctcac
     8461 cttgcttaag gtgaattata tattcgattc ttttcaaatc tatgtgttta tacaattaat
     8521 atttcggatt tttagagaat ttcggtgagt tgttttaagg aaaggaatag aaatcctata
     8581 aatcttctga ctcttaataa ttcgaattgt gacatttttc taatcgtttt aaaaagagaa
     8641 tatgaatttc ctcttccgtt ttttctggga agcgctttct aagcccctgt ttaaaaaggg
     8701 atttggtaag ttcgttcaac tccattacct tattaaattt ctctgttgga gtcattcgac
     8761 gaagagtctc taaatagatt tctttttctt ttttagattg ttttggaatg gaattatagg
     8821 catccatagt agacttcaaa gttttttttc gataatttga aacgggatag ttactatttc
     8881 tctaaactct tctgcgacct cttctaatac atcattctca ggctcataat accaatggat
     8941 attgatatct tttccttttt tataggcttt ttccaatgtt tccaaaaatt gactgattcg
     9001 acccgaggag gctgtattaa aatactttaa attaaaagaa acgatgcatt tgtgatggag
     9061 ttgtgcaaga taacgattta accattcaaa gattggttta aaaaactttt ctgcatcttc
     9121 tggatatact tcccctgtga tagataaagt gtttagtttt gcatcgaagt ctactttggg
     9181 agtagttttt gttttttcta actttaaatt ctccattatc caaaccgttt aggataaagt
     9241 atattcaggc aagaagaatt tagaggattc ttttaaaaag aaagaatcga gtatatcctt
     9301 ctggaaaatt ttcaatttgc cctaagattg agaaacctaa cttttcatag aatctaggcg
     9361 cttgaaagct aaatgtatct aagtatacaa cggtgctttc tgtttcccga gcgagtcctt
     9421 ctgctttttc catgagtatt gtccctactc ccttgtttcg ataatcctct cgaacccaaa
     9481 gaagtgagat atgtaaacct ttgtaataaa tttcgccaag cattccacca atgatttcct
     9541 ctgcttcttt tactaggata gaaaaaagac aattgtattc taatgaagga ttggaaagtt
     9601 tttccaatcc atgttctcga agccttgccc ataaaaaagc agattcattt ggatcttgtg
     9661 tcttttctac aataatttcc atgggtagta aggattaaaa aaacttatag ttcaaagaaa
     9721 atcaaatata aaaatcagat atttgtattt tataactaac tgaccttacg caaaagataa
     9781 aaattgataa gtatgataaa ggaaattaga atattagata aagctaataa attcaatagc
     9841 ctccggtata gctcggctga tggggagcga aaagcgatgg gagaaaatga gagatttttg
     9901 aactacagag aaaaattctc tatctaacgt cagttcgagc ttagatcatt tctctgcagt
     9961 ttctatttct tgttttaaag tttctctaac gcttttgcgt aatttggttc ttgtgcgatt
    10021 tctggaactt gttctgtata gaccactttc ccattctcat caatgattac aactgctcta
    10081 gaaaaaactc ccgctagtgg tccatcggca atacgcactc catatttttc cccgaactca
    10141 cgtttacgta gttcagatac gggaataacg tcctttagtc cctccgctcc acaaaaacga
    10201 ttcaacgcaa aaggcatatc agtagatacg cataaaacaa ctgtatttgg aaatttagtt
    10261 gcttccgcat tgaatttacg aaccgaagtt gcacagacag gagtatcaat acttgggaaa
    10321 atatttaaca ctactttctt tcctgcaaag tctttattcg taacatcgct catatctgtt
    10381 ttagtcaatg tgaaacttgg agcatcttct ccaatcttag gaagatttcc aactgtatga
    10441 attgtatttc cttttaatgt tatatctgcc atttttaatc cttttctact ctttgtatat
    10501 ttacagtgat ttcgtaaaaa gtgtatcctt aggaaaataa aagactacca ccgatggaca
    10561 cggatgaaca ccgataaaaa attatgaatt tattaaattc ttatctgctg ctatcaatgt
    10621 ttcttcgtat gtcataacag actctcacta tccgtaataa tccttacttc ggaactcttt
    10681 acacacccct gatcattgtt actatgagtt tatttattac tagtgctact ttgtcaagtt
    10741 cactaatttt tatttagact aggaataggt aaggaataag cagttgtaaa attttataac
    10801 aatctaggcg actttacaaa gtcgaaatat tgatagtatt tattcgatta ttttataatt
    10861 ttttcgaatg gtttttgcag aaaatccatc ataatgccac cactcgttcg gtctttgtat
    10921 gaacccagcg tcctgcatga tatttctaag tagaattcga ttctcccact gctttttggt
    10981 taattcgcct gattttaaaa aagcttcttc tttggttggc tcggataaag gtagaaaact
    11041 atcaaatgga gttcccatat ctaactcttt tcctgtttca tctagaatcg atagatccag
    11101 agcaaatcca taattgtgaa tcgatccaga cttagggttt gcaacatacg gctcttgtga
    11161 tgttcctttt actttgtccc aaagtttata ttgaatcgaa cgaggtcgta aacaatcaaa
    11221 caatacaaac ttaaattttg gctttttctc attgagtttt tgaatggctc gctttaattt
    11281 ttcatttgct tctctatgca gataacactt gttatatgaa ccatatagat tttctttcat
    11341 gaaattattt gcactcgcat acttcaaatc aatctgaatt gattccgaat tttgaatcca
    11401 aataaagtct gggcttttgc tcaaagcctc ccctactcta tcgtctgctg ctaatgtttg
    11461 agtaaaaaaa attacgagga atggtagtat tatcattctt atatataaat taaactctcc
    11521 ttattaaatc aacaacagaa gaatctaaat aaatttttca acagagacgt taaatatttt
    11581 cgcaagcttc tttgctgtat tcagactgat ggaacgggtg cctttttcca tatctatgac
    11641 atgccatttc caaatgccct gtaatcagga atacttgaat tgcggactaa ttgcagagcg
    11701 tcagtataag aatataaaag ttttagtgca taaggaattc tttttctaag atataaatct
    11761 tcttctaaag gattttcggg attatcattt aacacttgtt tggcgtttcg aacgatgatg
    11821 tgttctggga tgtaacagat ctgtgtgttt ttataactgc ttgctcttaa ctcggaaacc
    11881 ggataagctc ctcctcttgc gccagagaca gttacaatca gcccaggttt gtgggataat
    11941 tcttgtttat tgcagagaag aaaaaagttt ttaatcgcag gtgcagccat tcctccatat
    12001 tctggagata caatgagaaa accatcacag gtttttaatt ctttcgaatg gggttgccat
    12061 ttttctttcc aaacttctcc atctgtccaa gcagactctt cccaaagagg aatgcccgaa
    12121 gttcctaatt ttaaaagata gatgtctttt tgatctgctt ttattaaagt ttcaaaataa
    12181 gaactaacct tagccgattc cgaatctttt ctatgacttc cacaaataat tccaattttc
    12241 aaaattctca cctcttctgg aattattttc agactctgtt ttgaagaaaa gattattttg
    12301 ttcttttttg aatcacattt ttgatcttac cttttgattt tggaactggc tttcggatca
    12361 tcgcatccat tgcctctaat ttactcaaag ttgtgacagg tatcgatggt tttgagatcg
    12421 gttcttcttt tcctaggttt tctgaaacgg agtctttcat tctcatcttt tctggtagat
    12481 attccacaaa actaagatga ttggcaatgt gcatagaatg aaatttttcc caagtagctt
    12541 tatccatttt atcatacata ggatgagagg caaactcgcc gttaaatgaa cgaaatgcat
    12601 aaattgcttc tcttaattca ttgagtgctt gcatttcatt tccttctact ctatttgaga
    12661 ttgtatctcc attataacca tttggttcca tatatccacg ttttaaaagc ttagaaagta
    12721 gatacttgcc gagagttttt ctaaaaaagg ctgcataata atagggaaat ccattcatgg
    12781 aatagtgaat atgatcctta cagtgagtta aaatctgata aaacgaccac aaaccggttg
    12841 tttcaatata atttgatttt tctaaaatgg ctaaatggtc tagaacttga tctaaatttg
    12901 taaatgtata tctttttata aatattgcag tcataagaaa ccttctaaaa aagaaacaaa
    12961 taggtttctt aattttattt tttttacaag caaaataatt aaatcagatc ttttttattt
    13021 gaattttcaa cgcaaaaccc taccctactc cagattccgt gtatcattga gatagtaatt
    13081 ttttcaccac cgatgacacc gataacggaa cgataaaaaa cgatggattc ctattttggc
    13141 tgaatgctgt gattcaatat atttaaatga ccaatattta aatctttaga aacgaaatca
    13201 aatccacaaa tcctgtaaag taacaaaacc gaactttgcg taggtccatc ggttgcagtc
    13261 aggtttccca tacttctgag taaggaaatg atcgtttcca acctttacaa tcaataccta
    13321 tcctctggtc aaatccctct cgatagatct acatacgact gttaatttat aaataattta
    13381 tttacaaaaa aatattatca aaaagttaga aatggataag cacgataaca aactgataaa
    13441 aaacatgatt acttgagaat aatcaaatct ttcatcggtg ttcatcgtcc ctatcggtgg
    13501 tagtctttaa atggttttct taactaaatt agattgccct tgcatgttag tttctctagg
    13561 actaaatttc aatcccatat ttcttagcga ttggtcgaat ttcttttaaa aaagattccc
    13621 tttgtttttg gtttaatttt ttcagactga tagacgtttt atctttttta gaggatgaaa
    13681 tcatttcacc ccccctaccc tctttttgtt ttaagagttt ttctaaattc ttcaccgaag
    13741 gatcatttcc ctgtccaata aattcggaaa catgctttaa ttcgagtctt gcaatagaac
    13801 tcaactgctt tacatatctt tctgtataac ctaaaatatc agcaacttct aaagccgttt
    13861 tttttctttc ttttcttaga tattgaattg cctttccaac ttcccaggga tttaaacttt
    13921 tcctcttttc attttctgca agtgccattt cataacaaag ttctggattt gcttctgtta
    13981 caatcgctgg aatttctttc cacccaagtc tctctgctgc gtgaaatctt ctctctcctg
    14041 ctacgattaa atatttagaa ttgttttttc taactacaat cggctcaata agccccattc
    14101 gtttcatgga atcgactaaa tcatctacat tgttttttcc aaattgtcta ggctgattcg
    14161 gacttggctc aatatctttt aagctcagag tttgtgttgt ctttggctta ccctcataag
    14221 cggaaattaa atctaaagtt gaaaaatcat ttttctttga catgtatttt actcctcact
    14281 tcttttgcta aatctaaaaa cgatttattt gtttctttat cacatccatc cagcatcttt
    14341 ctcttggctt gggcttgtgg tatactttct tttctataaa tgctcgtttt aaaaacaggg
    14401 aaatattttt ttacactttc agaaagtcca gataaggctc tatttccctc gaattgatta
    14461 aatactgcac ctagcaattt taatcttgga tttgccttgt tttgaatctt ttgaattgtc
    14521 tcatgtaaat cttttaatcc ctgcacacta aatgccctag tttgaacagg aacaataata
    14581 tagtctgctg cgattagcgc attttctagc cctaatccaa gagagggagg acaatctatg
    14641 agaataaaat cataagagga aagcactggc aaaagagcat ctttcaaaag ttcaaaatca
    14701 tctctttcaa aaggagtcgt caaatttgca agtgacaagg aagatgggag caaatgaaaa
    14761 tttggtcgaa ttgggatgat gactgtatca acggaaaact tttgactttt tcggtaaccg
    14821 agacaatcaa acaccgttct ttccggttca ttcacaaaaa gtcgagtagc atttgcctgc
    14881 ggatcccaat ccacgagtaa aactttggaa tcaaaagaga gtgcttctga taaaaaaaga
    14941 gaaattgttg tttttccttc tccacctttt tggttcgaaa ttgcaattac atttgctact
    15001 ttatccaccg attgatttcg agtaaaatat ttatctagaa tctctgtttg atacttacct
    15061 gatttagaaa ctggaatttt ccattctttg attttctttt gaagaccaat tacggatact
    15121 ccataaaatt cggaagcttc ctctatactt aaatcgactt tctctttttt tttcatatga
    15181 attattccaa aataatcatc gactaatttc ctaaaaagca aaaaaagaat ctaagaataa
    15241 ggggtgaaat gatttcacgg taaaatcttt cattctatca tttctttgat ttacataatt
    15301 tagattgtga gggttaatgt tttggtgaag ccatatagta tactttttat tggaatgagt
    15361 ttttttatcc ctcttttgca aattttcggg gatggcggat ttgtaggtat cattggagcc
    15421 cgaaatgaag gagaacatat ctttgagaca ggcaataaat atccaaacct ttctggaatc
    15481 agaggtggat ctagaataac ctatcagcgg aattttaact tagccggaat tgaaggtggg
    15541 tattttaaaa acaaatattc ttttcgtggc agagtgatct ccaatttttg gaatcaaaac
    15601 tctggtggaa caagagatga agactttttt ttaggcaata gttcatctga aaaaggttcc
    15661 aaattttctc catttcaagg agtcctacat gacacagcat acacatttac agggacagca
    15721 aactttgcag acgggaaagg tagatctacc atttcggaat acaatctaga aggatttttc
    15781 agatatcatt ttggtgatgc atctcctgat ccttgggcaa ctggttctgg tttttttctc
    15841 tcaggcggtc tcagatattc ttatgcgaaa ctccaagttt atgatgtgat ccaatatgta
    15901 gcatctagac ccatatttta tggtcctatt ggttcaggat tatcctattc ctattctttc
    15961 acagaaattc cagttggagg tggttacatt tttaatttag gtaattttaa aatagaacca
    16021 tctgttcact tactttatgc ttttattaaa actagggact ttcatattca aagagcactt
    16081 aatttcttag cacacacagc aggtcctgga tttctagcaa gaatcgaagg tagttacagc
    16141 atctctgaaa atgcaatgat aaaaatagga gtcacaggac acaggcaatt tacaggaggc
    16201 tcctttaaaa cgagaggagg actttatgaa aatgatatcc tctcaaatta tttaggttcc
    16261 tataaaagtt atataaatac aaaagaagta atctttgaca tgtcctatat aatgcgatta
    16321 taaaaagatt gacttgtata tagatacaaa tccaaatgaa tcaaccgttg ggggtgggta
    16381 atttttttac tctgagagag tccccaatac ctgatctgga taatgccagc gtaggaaaac
    16441 ggaatagacc cttttgattt attctccaac ggattccata catggacgaa taaactatga
    16501 acaatccaaa ccaatttcaa tcactcgcaa aagacttccc tctaaaagaa aaaattcaat
    16561 tagatggaca cgaaattcct gaaaagaaaa tcaaactaac aaacggacaa gaagtctgtt
    16621 tatacaatac agaaggacct attggttttg atcccaaaaa aggaattcca aaaagaagaa
    16681 ttgagtgggt caaggaaaga aagagtaggg gagatcaaaa cttttcccaa atgcattatg
    16741 ctagaaaagg aatcattaca gaagaaatga aatttgtagc atttagggaa ggaatcgatc
    16801 ctgaatttat tagaagtgaa attgcacttg gaagagcaat cattcctgcc aatataaatc
    16861 atccagaatt agagccaatg atcatcggta gaaactttct ggtaaaaatc aatgcaaata
    16921 tcggaaactc tgcaatcact tcctctatag aagaagaggt ggaaaaacta aaatgggcaa
    16981 tcaagtgggg tgcggatact gttatggatc tctccaccgg aaaaaatatc catgaaacga
    17041 gagaatggat tattcgaaat tcaccagttc caattggaac agttccaatc tatcaagcac
    17101 tagaaaaggt gcatggcaaa gctgagaatt taaacataga tttattcatt gaaacgttaa
    17161 tcgaacaagc tgagcagggt gttgattatt ttacaattca tgctggagta ttactcaaat
    17221 atataccttt aaccgccaag cggataacag gcattgtctc ccgaggtggg tctattatgg
    17281 caaaatggtg tcttgctcat cataaagaaa attttctata cactcatttc gaaagaattt
    17341 gcgaagtaat gaaaaaatac gatgtttcct tctcactagg agatggacta agacctggtt
    17401 gcacagcgga tgcaaatgat gaagctcaat tcggagaact tcagactttg ggtgagttaa
    17461 caaaaatcgc ttggaagcat gatatacaag tcatgataga aggtcccgga catgttccaa
    17521 tgcatttgat ccaagagaat atgcaaaagc aattagaaat ttgccacgaa gcccctttct
    17581 atacactggg tcctcttgtg acagatatcg cacccggata cgatcatatt acttccggaa
    17641 ttggagcagc tatgattggt tggatgggaa cggctatgct atgttatgtg acacccaaag
    17701 aacatcttgg tcttccgaat aaagaagacg ttaaaactgg agtgatcact tataaaatag
    17761 cagcacatgc tgctgattta gcaaaaggac atcctggagc tagaaatcga gacgatttat
    17821 taagcaaggc tcgattcgaa tttagatggg aagaccaatt caatctctct ctcgatccag
    17881 aaacagctcg taattttcat gatgaaacac tcccgcaaga aagaatgaaa gaagcgcatt
    17941 tctgctccat gtgtggtccc catttctgct cgatgaaact gacccaagat gtaagagatt
    18001 ttgcagagaa aaatggctat tcccaagaag aagtctttgc aaaaggcatg gaagaaaaat
    18061 caaatgagtt taaagaaaaa ggctctcatc tctatacagt gagtcaaagc taagctttta
    18121 gaattcattt tccaacccat aaaaattacc ttttacaaaa agagtcgatt gtgagacatg
    18181 gaagcaatca ctcttttgta ggcaaataat gaaagtaaca ttagtcaacc taggtggacc
    18241 tagaacctca gatgaaattg aacaattcct cttggatttg ttttgcgatc cccttgtttt
    18301 cgatctccct attccagagt tttttcggat tcgacttgca cggtttattg caaaaaaaag
    18361 agcccctaaa gtagcacatg cttacgcttc aatgggattt gggggaggtt cacccctagt
    18421 tgctgaaaca gaaaaacaag ctaaggcttt agagaaaaaa ctgatcgaaa aaacaaagca
    18481 ggaatggaaa gttcaagttg ctatggcttg cggatatccc aacctaagag aacttcccaa
    18541 agaggattta attccttcca aggataatat cattctacct ctgtttcctc aattttccag
    18601 atcgaccaca atgagtatcg ctaagttaat cgaaagccaa acaaaccaat gccctgcgaa
    18661 tctacaaaac ggtagttgtg gaaaccactg tggaacaaat aaaaagggag cgatctgccc
    18721 tttaaaccga aagggtttcg tagctccttt tcacaaagac ccaaaattca ttgaagcaac
    18781 tggaaagtta attctagatt atttccaagg gaagttaaat gctaaagact tccttcacct
    18841 agatactgca aaaggaattg aaaactggca agaaattacc attgtctttt ccgcacacgg
    18901 aattcccatt cgactcattg aaaaaggcga catatacaga actgaaattg aagagaatgt
    18961 aaaaaatatc acagcagaac taaatcgatt tggatttaga ggcaaatcat tcttatcctt
    19021 tcagagtagg gtaggaccta ctaaatggac agagccaaat acaattcgca tgctagaaga
    19081 attaggaaaa aacggacata agaaagtagc cattttccca atcagctttg tgagtgatca
    19141 tctagagaca ttagaagaaa tcggagttca gttaaaagaa gtagccaatg ctagtggaat
    19201 tacggactat tttagaattc cagcacatgg aatttatccg gattttattg aggcgcttgc
    19261 atctattgtc ctgaaagaac gggattgagt tagtctttag atccatacta tcttgctagg
    19321 atctttcaaa cacgaagtta agggttttaa atttcttttc ttcgtgttct attgctaaaa
    19381 cccaaaattg ctcttcataa aattagataa attccttctt tattttttag aattggagag
    19441 ttttgaaatc tccataaaaa atcagactcg caaattgtaa tttactaaca aaagagtctc
    19501 gaaagaaatg aatcgatacg taaaagtaag aatactcaat ctaattcaat atcaaagtat
    19561 taaaattaaa aatcaaatca gatcttctta agaaaaattg tgtaaatact tttggcagta
    19621 agggaatagt caggaaaaat aatttatttt gaatattttt cttgacttaa ttttcaaacc
    19681 gttacgagtt ttgtaagtgt acttgaatca acaatagata tttgaaatta atttttccga
    19741 tataaaaaaa agctaaaaga agtttttgaa attagtaata tcaaattcaa tattcaaaat
    19801 tcaaacttaa aactaactgc caaattcaaa ttagagagaa gagatttatg agcatatttt
    19861 catccatcga aagctacagt cgcaatgtca aaattagaac caaaatgatt attttgattt
    19921 caggggtagt tttaatcagc gtgctacccc tttctctgat tgtattatat cgaaaccaag
    19981 cagtagtctt aaataaaacc ttcgaagtgt gtagcaatct cgctcaaaac atctcaaacc
    20041 ttgctaccga agaattacta atgaatgaaa cgtatgatac cactcgaact tcgattaaaa
    20101 ggctaaaaga cagtaatatt tctggtctct tagaatcata cgtagtcaat gtagatggaa
    20161 aatatgtagc cgacttgaat gaacaaagaa tcgatcagca aatttctgat tcagacaaag
    20221 gctatttcaa aaaattagaa aaattagaga tgaaggaaat caattccaat tctaatcagc
    20281 caatccttcg tttctcctat ccaattttta tcacagaata tgaaacaaag gaaatcagaa
    20341 aggtaggaat tgctgtcttt gaatttgata aaaagaaagt atatgaacca gttgtccaaa
    20401 ttcgaaccac tatcatcgga gttgctgggg ttttatttat tgtagggatt atcattgcgg
    20461 catacgcggc atttgccttt tctaaaccaa tccaaaaatt atccgaaggt gcacaaaaga
    20521 ttgggggagg ggatctcaat catagaatta aactaactgg atctgatgaa ttaggacaac
    20581 tcgctaaaag tttcaatcta atgacttccc aaattcaaga cttcacacaa aacctagaat
    20641 taaaagtcgc tcaaagaaca gaagaactaa atagaacttt gcaagaagtc caagctctaa
    20701 aaattgcaca ggatggagac tattatttaa catccattct attaaaccct ctacaaccta
    20761 acaacaatcg tagctctaaa gtaaaaactg agtttatgat tgagcaaaag aaaaaattct
    20821 cattcagaaa gtggcactct caaatcggag gcgatatctg catcaccgat acgatcactt
    20881 taaatgggaa agaatataca gtttttatca atggggatgc aatgggaaaa tccattcaag
    20941 gagccggtgg agctcttgta ttgggtgttg tatttaatgc aggtctcatt cgatccagaa
    21001 ttgaaaaatt tcaaagcaca tatcctgaaa tctggctcaa agaaagattc ctcgatttac
    21061 ataacgtatt tttatccttt gatggatcta tgtatatttc aatttgtcta ggtcttatag
    21121 acaactcaac gggattgctc tattacatca atgcggaaca tccttggaca gtattatacc
    21181 gagatgggaa agcatccttt ttggaacaag aacttgctct tcgaaaaatt ggaactccag
    21241 accaagaaga tcaattttat gttcgtatgt tccagttaat gcctggggac gttgtgatta
    21301 ctggttctga cggtagagat gatttggtct taaaaaataa agaaggaatt gaatacatcc
    21361 aagaagatga aagccaattt ttactcaggg tagaagaggg agaaggcaaa attgcacaga
    21421 ttgctcaaag agtaaaagaa actggggcac tgatggatga tttttcgctt cttagaatat
    21481 cattcaacga agaatatgca gaactagaaa atacaaaaga aattccttat catgtcacag
    21541 aatcagtgag tgcaggtatg gaattattag aagaaggaaa tgcagaagaa gcaattaaaa
    21601 aagtagaaca atttctacat gactacaatg actttccaga tatgctaaaa cttttaggaa
    21661 aagtctattt tcaaaaagga gatttcccga aagcaattga atgctttgag caatatttaa
    21721 ccttaaaccc aagtgataat gaatatctat atgctttatc taacacatat agagtttacg
    21781 gcaaattaaa cttagccgct gacatagcgg aaagattatt tttaagagat caaaagcatc
    21841 tcctaaatct aatcaactta gcaactattt attacggtct tcgtgtcttt ggtagagctg
    21901 aatctatgat caaacgagca ctctatatag acccagaaaa taaacatgca gaacttcttt
    21961 atgaatctat cagagaagcg atcattgaga aatcttctaa taacaatggg caagtaaaga
    22021 aattaacact ttcagaaagg ctaaaggcag agaatgaaaa tctacttgaa caagcagaag
    22081 aattatataa gaacaaagat taccaaggag ctttaatttt atatcaacaa ttactagatg
    22141 acgaaaacag tgagaactct agaattcttc tgaagatagc gaactgcaat tctttcttga
    22201 ataaactaga tcaagctatt attttttatg atagagcatt gaaaacagat ccgatgaatt
    22261 atcatgctca caataattta ggtggaatct atttcaaaca aggtctctat ctcaaagcaa
    22321 aaaatcaatg gatgaaaagt ctagaaataa aaacagactt caaaccagct gaaattaatt
    22381 tgcaaagact tgaaaaaatg gaaagtaaac ttagctaata aaggttaaca ggaagtatac
    22441 gtatggtaag aaaaataaaa ctcattttaa cactctcact ttttttgaca catgcaatct
    22501 atgctgataa accaatagaa gttttactct cttcagacaa tagaatttat gaacaaggac
    22561 tctatggtat acaatctgtc tttgaaggtg aattaaaaat atcctattta gatattatta
    22621 ccgcagaaca acctgatatc gccagctatt ttaaggcgat agacgactca ggggctcctt
    22681 tatttattac aattggacca gcagcaacaa aagcagcaaa agaaaattta aaaaaaaccc
    22741 ccatagtttt ttcgatgatt aattctccaa aatcccttgg aattgatgga ggaaatcttt
    22801 gtggcgtaag tatggatatt agcattgggg aattcttcca agcattaaaa gatattaaac
    22861 catctgccaa aacagtaacc gcattttata ctacaaatga tggagagtat tctgctggag
    22921 aaggagaata tactgatcta aaatataaac tcatttacaa tcgcaaaaaa ttagcagata
    22981 aaaaagaatt caaaactgct ctagaagaga taaagggaaa aacagatgca ttcttcatgg
    23041 taaatgatcc actttattcg agtgttgaat tcgaaattct atctgaattt gcaaagaaaa
    23101 atagtatcat cttgatgact agctttcctg ccttagtaaa agttggagct acctttggta
    23161 taagtcctga ctatagtaaa atcggtgtat tgactggtca aatggcaaat cgaatcaatt
    23221 ctaagtcaag cacatgcagt gaggaaagag ttctactgcc agatcaatcc tcttttttct
    23281 taaatgaaaa gtatgcaaaa gattctggaa tcgatcttcc tgaagcgata gttgaaagag
    23341 cgaaacttac aaaattattt gatgtgggtg ttaatttatt taacgaagga aaactaaatt
    23401 ctgccaaaat tgtatttgaa gctattttaa aaagagatcc aggcaataaa tcagcatact
    23461 ccttccaaca actgattcta gaaagaatgt ctggaacaaa aacgagagag ttattggcaa
    23521 gtgcagacaa tcatttcaaa aataaaaatt atgcacaagc gagggctgac taccaaaaag
    23581 ttttagcaat caatccaagt attggtgcag ctaaagaagg aattcaagca agtttactag
    23641 cccaaagtga acaagagaga gcgcaaggaa ccgatttagc aaaaagagga aaacccttcg
    23701 aagcaattaa aatgtttatg gcagcactga gaactctccc ttccaatgca caagccaatg
    23761 cagatttgaa tgcaatcaga aattttgaat ctcctaatat gaagtcttat atagagaaag
    23821 gaatcgctca ttacaatgac agaaactata actcttctat agaaattttt gaaaatgcat
    23881 tactcgttgt tccaaataat aaagaggcaa tcgaatactt acgactctcc tacaaaaaga
    23941 gagatgcaat gatcgtatta tgaaagaaat tagatactca atagattttt aaataaactt
    24001 taagaattca aataataaat cgtttttaaa cttttgattc ttagaaacca aatcatttct
    24061 tgtgctaggt aactctaata tcagcaaaaa ggatgtgatt ggagtttatt ttagatatat
    24121 tcagattaga tttcaaattc aagtttgatc accactcata gaagaggaat atctcatatc
    24181 cctcttctta tacaaatcta ttgtcttcgg cattcataat acataaaaca cttttgagat
    24241 cttgtatatt ttactaaatt cacaagcttt ctttaaaact tttatcacaa atccaacaga
    24301 acatatagta tatcaataaa aggtctttcg atagattcta caggaaatct aaagtcataa
    24361 agacttgaga acttttttta aagaaaccaa atcaaaacct tctttatata gcttacttcc
    24421 ttcagggaaa tatcttccgt ctatcgcact atttttaaca caaggtagaa ttatgcataa
    24481 ggatatattt tttacataac aacagaaatc gaataccttt agtcttttga agcgaataat
    24541 agtcgttatc cgcaataaat aaaaaatatt tatattttat aaaaaattat tatttctgtt
    24601 tgacattata aaatgagaat tgtatgaatg gaaaagaatt ttccatcgaa aataaattca
    24661 agcgtaaatc aataatctcc tcacgtggga tactgtgctg tcgcgtgcac atttaaatat
    24721 ataaaacaaa aaaagttttg aacaaaactt tcaagaggat ttcatgagat tttttgctct
    24781 cattagtata gtatttttaa caactagctt gtttgcacaa gaaaaccaaa ataaatctaa
    24841 gatcatttat attacacatt ttaatgcata taaatctcaa aaatctgatt ctatttcaaa
    24901 taagatttac aaaagtttgg aagaagattt caaatcactt ggatatgaag taagagaatc
    24961 aaatacagat ctatctagca ctcttaaaac tgccaaggca aacaactcaa tgtttgtgat
    25021 tgatggatat tacaaactaa atgaaactgg gggacttagt atctactcgc aaatatataa
    25081 tccagatact ggttatatga ttgatgcatt aaatatcaca gatgaactct acggagcaga
    25141 aggagtttca ttagatccaa aagaaacaca gaaaccagca gattctacta tagatgaatt
    25201 caaaaagaaa gtcgtaatcc gtattcgaac aaacacaaaa agaacagagc gaagagaaaa
    25261 tatcaatgaa gcagtaacaa ctacagctct aggaaaagat aaagacttaa aatttccaat
    25321 tgcagaagaa aatgttgcta gcgcatctgc agatgtcttt aaaatcttag cagaaaaaga
    25381 aacagtatct gtagcatcta acgttattaa agacgcgaag aaacagcctg tatctgtttc
    25441 aatcatcaca aaagagcaaa tcaaaatgag tggagccaga actgtaaatg aggttttaac
    25501 aacttacgtc cctggatttt tcactgtgga agaccaagat gatacaattg ctggctttcg
    25561 tgggttcgca cccgatagca acgcaaaagt tttgcttcta atcaacggtc acaatatgaa
    25621 tacagagtgg ttttggggtc caccagactc aatcatcaat ggattaaaca tggaatacat
    25681 tgagagaatt gaagtaattc gaggtccagg ttctgttaca ttggggcagg gcgctctatt
    25741 aggtgtaatt aacattatca caaaaaatgg aagcactaca aacggaacaa cactttctac
    25801 taaactagga cagaacaatt actctattgg aaccttacaa gctggaggga gtggaaaaga
    25861 aaaccctgat ttaaaaacat ttttccaagt gtctacagct cgatacaatg gacaagaact
    25921 gagaagcgaa ggatgggcta gaacacaaac tctttctgga caagaaggat actatgatta
    25981 tcagcgcggt cttacacaat tagtgccaac ggctgatgtc tctaaaaaat attctgatag
    26041 tttgagactt tttgatactt acactcctga tccatctgga ggattcaatt ctgtgatcac
    26101 tcgaaggaac gtggcaacta gtggagcacg gctaaagaga gcagacagtg atgtagtaac
    26161 tggtgttatc aattataaga atctcgaatt aacaggattc tatacaaacc aaacaagaga
    26221 cttatataat ttctatcgag atagaaatcg agtgcaaaat acaataaaga gtggaaccat
    26281 cacgtatacc tatgatgtga gcgataaagt ttcacttaaa tttaaaaatt attatacagt
    26341 ggatgatatt tttcttagaa gccaaaaagg actaacgtta ggtggcacca gagaatatag
    26401 atatggtgga tcgatcatat taggactcaa tgatctaatt aaaaataaca ataccgccat
    26461 tggtatagaa tttagaaaat atgatatggg gcaagtaaac tctgatggga ataatttcat
    26521 actaaattac tctcaaaata cagcagacaa tgctttgcta ttggataaaa atggactcac
    26581 tccaaatgaa agaaatcgct atgtctatcc aggtagcatt gcagtaagaa gtttctttat
    26641 ggaggatttt tataaactgt ctgaaaaaat agatattttt ggagcctttc gctatgataa
    26701 acatccctat tggggaagta acgttgctcc ccgcgttgga gcactctatg aagcaaataa
    26761 agatttgcga tttagatttt cttatcaaga aggttttaga ggtgtagtag gggtttccta
    26821 tgcgggagga ttcgagggcg atggccatct tagaattcag aattttcctt atattgaagc
    26881 atctaacatt ccttcttctt tcgacaaaca aggttatcct tctggttttt acagagatgt
    26941 cccaaaaaca aagccagaaa aaatgaagag cttcgaattt gcaacgaact actcctttac
    27001 ttcgaactta tcgcttgaga atattttgtt ttttaacaaa gtagaaaaag taatcgatgt
    27061 aggagttttg tattgcgata aaccaaaccc gacagcaaca gccccaggac caaatggatg
    27121 caatatgcca agacttggaa atgatacacc tggaaactgg aatggatatt ggttctataa
    27181 aaataaccca ggagaaatca gacagggtgg agctgagatt tctttaaatt acaagaacag
    27241 attgatttcg gcaactttta gccaatctat tgttaaacta ctaacggcat cgccaggaca
    27301 aacagacagt atttatttaa caagcgatcc aaacaatcgc caatttagag gttatcctag
    27361 taacgttacg cgactacatg cattacttta cccagtagat aaagttacag tatcttttac
    27421 ctacattttt tatccttctt ggtattctcc tacaaacgaa agagtggaag gaaatcattt
    27481 ggcaaattta ggatttaatt acaagtatct agaaaatttt gaatttcatt ttatactgaa
    27541 gaatcttttt gcggctggca atttatatcc gatgataagc aatgctggag gtcctgatct
    27601 ttcgaatgga acaccagcag ttgaaaaaag aactttttgg ggagggttta gctatacatt
    27661 ctaaaaacca ccttgtaaca atcctccacc tccaaaaaaa tcttttggag aaatgttaca
    27721 tgtaaatgct gctgagtctt tacatgaaat aaaaagaact tgtttaacac atgaatcaac
    27781 atctttggtt gcatagtatg ctccatccag aatttttctg tcgctcgttg taagtctttt
    27841 tactaggagt gcaacgttag ttgaatcaaa accacaagaa gcaaatttaa ctaaatatgc
    27901 atcatttatt ttatttactg catcatgcct agaaataaca tctggtccat ctataccata
    27961 ataaactcca gtgcagcttt gagcgattaa cattaaaaaa agtatatatt tgtttttaaa
    28021 aatcataacc acattctgaa taacaaatca aaaaaataaa gaataatttt aatttaaaat
    28081 tataaaatat attttttata taaattaatt ataatcaaat aaaaataaaa gacctaattt
    28141 tctaaaattt aaacttctaa attcaatttc ttacttttta aaagtcataa ttatcatgat
    28201 acgatagtcg atttatctta gtttaatttt ctaaagtaaa tgtcatttat ttttatctag
    28261 atattgatag aaccctaaaa caaatgaaat ttctagaaac ttgttattta agtttttcaa
    28321 aaatggagat ttttgaattg aatcaacttt ttaaaaaata gcacgatcaa aaaacctagc
    28381 aatcaataag caaagaaggt aaaggcgcaa aaaattcaga gtagggtaga tgatcttaca
    28441 atactaattc aaacctattt ttcaaatagc gaaataaaaa cattcttact ctcgagagca
    28501 atctacaagt ttttttgaaa caactgaagt tagatttccc tagtagaatc aaactttctt
    28561 tccaaggcta attctgatta acctccggca attcaataaa gtaattttgc ctagtattaa
    28621 aaaaatattc cttttttatt tcatttaact atgtgtataa aaaagttgag ataggttcta
    28681 ttttaaagaa ataactatat tctcaaatat ataaatctac ttttgcgcta taggcatttc
    28741 cctataatac ctcaacgatt ctagatttta taattgacaa tatttttaat ttacattcag
    28801 agtgaaaaca cactccaaga taaatgcgtt aagaattgaa tgatatgcag accaatcatt
    28861 tgacaaagta ttttacaata aggaaattta tgaaactaca tatacaaacc ttttttttag
    28921 ctactttctt tttgattgcg attcctgttt actctcagga caaacagaat aaaaagattt
    28981 tcatatcaac attctctgca tataaatcga ataaagattc aaacgtatcc aataaagtgg
    29041 tagaaagttt aaccagtaaa ttacaatccg ttggctatga agtttcaaat atagaatcag
    29101 aaaatttaaa agacaatcta aatcaaagta aacaaaaagg agcaggctta cttatctcag
    29161 gattttatag aagagaagat aatggaaatt taagtctata tggacagatc tacaatccag
    29221 ataaagaagt tatgattgat gcactgaatc ttacaaccga tatctctgga atagaaggaa
    29281 ttgcactgga tccaaaagaa attaaaacat cagatgaaca aaatatagat gacttctcaa
    29341 caaaaattac aaatcgaatt aaagcaaata taaagagatc agaaagacat gaaaatataa
    29401 atgaaaatat agtaaaccac ccaataggaa aagagaatac atttcccctc cccaaagaag
    29461 acttaaatgc tacatctgaa gaggtcttta aattactttc agaaaaagat gatgtagttg
    29521 tatctgtttc taagttttct caaaaaacat ctgaggctcc tgcagacata acagtaatta
    29581 gccgcgaaca aattcgaagg tcaggcttta gaaatcttac agaagctctc aactttgttc
    29641 cacaagtcta tactcactgg attggacaaa actggagttc tgactttaga ggcttatttg
    29701 taaataatca aatagaaaga cgagttctat atttacaaga tggtaaaaaa ttaaatgatt
    29761 acttccactt tggagaattc tactctgatg tttatacaga tatggagcgt atagaaaaaa
    29821 tcgaagtaat taaaggaccc ggagcagcct tatatggaaa caactctatc actggagttg
    29881 taaacgtaat tacacgtaaa ccaactaaaa aaaatgaaat ggaacttgta actgaatatg
    29941 actctgtttt aagaaatcta accacaagag cattatacta ttcaaagttc agcgaaaagt
    30001 tttccgtctc acttgacgca tccagatttg agggtaaagg gtattatact tctggttata
    30061 actcttgggg aggaactaga tactatgatc caaatagtgc agctaacgcc cctaatttca
    30121 acccaacacc tgctaactct acttttggaa cgagtgaata tggaagaaac acggcagagg
    30181 tggagactgg acagcgggca tggacttcca caggtcttat ggcaaacaac gggaagtggt
    30241 ttccaaacta taacttagac gttaaatacg gagattggaa tttaagatcc ttttatatgt
    30301 ccaagagaac aagttggatt ccaccacaat ctgattctgg agcttttggg ggagatacag
    30361 tttacggttc ccctagaaac gatagaattt ggggagtagg ggtcgtttct ttagattaca
    30421 caccttcatt tctcgaaaaa tatgaagcta gtttcaagat attcaggcaa atcaatttaa
    30481 atagtgacta tagagaaaaa gattatcagg gctttgctca agctacaaat cctccagggg
    30541 ttccaagctc ttatggttca actccagcaa cgcgcttaac ctctcaaacc tatctaaatt
    30601 attttcaatc catgggtggc ggtgtcgtta aacgctactc ctcaacagca aatacacagg
    30661 gaattgaata ccaagtcact ccctataaaa tagataataa agatgctatc attaaatctt
    30721 tccgctttat gactggtgga aacatgcaag cagtgaacta tatcaattat caaacaaatg
    30781 ttggtctaaa tggattgatt gatagaaggc aacagggtat tgccgatgat ggaagacagt
    30841 ttggtatatg gacgcaatta acatccactt ttaaaacaga taccacaatt gttcttggac
    30901 tgagatatga tgctcagaaa atctataacg tctatcgaca tcagaatgga atgttaaatg
    30961 atgttgcaca agaatctgtt acggatcctt atcttagaac tcctagcact ggtggaaacc
    31021 aaccctttgt tggaccaggt aacacgatca atctaactac tccagtcaat gatgtatttg
    31081 gaaatagagt tgctaccgga tatgtgcaac cttttcaaag aaagaacttt gttgcagaag
    31141 ataaaactcc tagaattgcc tttattcaaa atattaaagc aacagacact acaattaaat
    31201 taatgtatgc ggaagcattt agaatggtta ctccacagga gttgattcga ttgcctagag
    31261 agttaggaaa tgctcaatcc gaaaaagtta gaaatagaga aataaacatt attcaaccac
    31321 tctttaaagg agcgttagtt ctcaattttg attattttag aatgactgga agcacaattt
    31381 atgcatttaa tgctgcaact cttgcatttg gtcaatcgcc tgaatggagc aataatggag
    31441 gatctctatc tgttacctat gtagttgaca ataaatggag attaaatgga agttattcta
    31501 gttatcaact tagaagacca agtgattcct cttttctcaa tactcttttt acaccaaaat
    31561 cacaagctct caattctcca acaaaactat ggaaggcagc tatttctaga tctgtcataa
    31621 acgataacta cacaatcagt cttgagttct actataacgg ttcaatttat ttaatggaaa
    31681 atccccctag aacaaatagt caagttctaa acaacgatgg cacacttaga gaattaccac
    31741 c
//
LOCUS       DAOQOS010000060        31721 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_628_length_31721_cov_6.778437, whole genome shotgun sequence.
ACCESSION   DAOQOS010000060 DAOQOS010000000
VERSION     DAOQOS010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 31721)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31721)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31721
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_628_length_31721_cov_6.778437"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            66..1355
                     /locus_tag="PLF66_14010"
     CDS             66..1355
                     /locus_tag="PLF66_14010"
                     /inference="COORDINATES: protein motif:HMM:NF012307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase/maturase family protein"
                     /protein_id="HMZ65208.1"
                     /translation="MISSPSNFFSVENLHKAYLKCRKGKRGKIHTRGFEIELYQRLNE
                     LSLSLQKQSYKPSRSVCFYVSKPKLREIFAADFGDRVVHRLLIDLIEPYYEKKFITDS
                     FACRKEKGTHRAVLRLKEFIRIGTNHSKKPLYYLQLDIKGFFMEIDKRILCSIIEPEI
                     DQTLQWLCKTIICHNPTKKFIYRGKVVKKGILPPHKTLFHEDENKGIPIGNLTSQFFA
                     NVYLNELDQFIKRGLGIKYYIRYVDDFLILHENPEKLMHWKEKIIQYLYQKLKLTLKD
                     DVMPLSVYKGIDFLRIPRVLSRFSRFRFQIRYYMKEFSKHLLIVQVGKFFELYGAGLE
                     EVIPYLPFKPRYRGQMKTFGIPIKKSKYAKNYLKDILKICKRKYITYLILTQSDEQAE
                     SVKIRMPRYQQQLIHPIQLELFNNSQKFYKYRRNKNV"
     gene            1348..1821
                     /locus_tag="PLF66_14015"
     CDS             1348..1821
                     /locus_tag="PLF66_14015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004789234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HMZ65209.1"
                     /translation="MFKKKIISNVSFTLLLLLLAKQIAATPFTDNGDGTIKDSASGLV
                     WQKCSSGQNATTCSGTATALTWATAITACNSLSLASKTWRLPNRNELQSIVDYTKTSA
                     PMIDATAFPSTQSSYWSSTTYAPFTSFAWYVYFSVGNVLSNFKTDIYYVRCVSGP"
     gene            2194..2457
                     /locus_tag="PLF66_14020"
     CDS             2194..2457
                     /locus_tag="PLF66_14020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65210.1"
                     /translation="MEASKLQINYKELLFKISSFPEETTRQLLEYIDFLWERHSPAVD
                     GLSELDKNELDKRVKKYFQNPETGFSLSQVISRLEKDLGEKLS"
     gene            complement(3118..3345)
                     /locus_tag="PLF66_14025"
     CDS             complement(3118..3345)
                     /locus_tag="PLF66_14025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002178217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HMZ65211.1"
                     /translation="MDVKEYITRDPKIMFGKPVIKGTRIPVDLILEKLALGETIEQLL
                     LAYPKINQVSINACLLYASLLIKNEVVYSAA"
     gene            3474..3740
                     /locus_tag="PLF66_14030"
     CDS             3474..3740
                     /locus_tag="PLF66_14030"
                     /inference="COORDINATES: protein motif:HMM:NF015630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0175 family protein"
                     /protein_id="HMZ65212.1"
                     /translation="MLHRTYIIDRTDLIIQIPETAFSITRSDKESFAKELLLAAAMKW
                     YEMGKISSGKAANLVGMNRVDFLYRLKEYNISTFQYTAEEILEE"
     gene            3751..3948
                     /locus_tag="PLF66_14035"
     CDS             3751..3948
                     /locus_tag="PLF66_14035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65213.1"
                     /translation="MPNFISNTSPILYLSKIELIEKNYSPSENFIIPNGVKEEILSGT
                     VNDSAKQWIQNPENKYCYVKN"
     gene            4042..4257
                     /locus_tag="PLF66_14040"
     CDS             4042..4257
                     /locus_tag="PLF66_14040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65214.1"
                     /translation="MQTILIQVTNEKAYKILADLEDLNLLKILKQMDTIPKKLSQKYA
                     GKLDSTIIDEFQKFVQNGRNEWNNASI"
     gene            complement(4713..5996)
                     /locus_tag="PLF66_14045"
     CDS             complement(4713..5996)
                     /locus_tag="PLF66_14045"
                     /inference="COORDINATES: protein motif:HMM:NF014305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HMZ65215.1"
                     /translation="MKLYFKIFLFFIFIITNESFAQIKLDNIFTYEDGISYSTVLPVN
                     YNKNKKYILAIGLHGLFSDGSRMVNPFSYYSRYMNIILVCPDGNFTDNGRNGVKWGYD
                     KSDKYILNLVELIKTKYKVYDDVFLFGFSQGGNQGLQTALTNPDIFRYFAGLSGGYTS
                     LTDKQISTAGKINILFISGDTGEGEAFTKREMDNRFQVLKRYNPYAIRKVYPGMRHEV
                     TPEEAFYMFDWLMNVNQKDRSTHIIKEDYYKFYLDSANALTNAKYQESIQYAYNSIEK
                     NKIFAPSYFNLISAFFGMQTIPNFKKYFFQTLELYSAYDFFDVVPLLNLIENIKLSDV
                     DFLLKEKFLEFFQTKMNEHEAKLSPLFKGEVYLLIAELYIISHNPDLARASFKKALSF
                     YSQIPSKNLIYLDGQVKKKILYITEYIKELEVSGK"
     gene            complement(6005..7681)
                     /locus_tag="PLF66_14050"
     CDS             complement(6005..7681)
                     /locus_tag="PLF66_14050"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HMZ65216.1"
                     /translation="MNYFRNFIFLSILLSLVSCKKNLELEWIKRSATKDIDLFLTPNK
                     FNVLEQDWIKIKHTVRYDKSDLIRHLKTYGRTIDDLKNNPELQYTESDNKVTILAGRT
                     AHSTHYEYDSSIPVIFYGPNWIQKGEYSEPIHQQHIVPTLAKILKIRNPNGVETSSLN
                     QILKTGTNANLPEIIVTVVLDQAGQQYYKAHPEIPIQISKLKRESAYFPNAHVAHIDA
                     ETAVGHAAIGTGAYPRKNGIIANGYLRFSNGKFEKDEIYTLTEDTVNPEELWTETLAD
                     VLDNEYHGESEVVSQCYALRASIGMAGHGAAKIKSVDYIGDKDLVYWLATSQSKWVTD
                     NRYYSLPSETAEYNPLKTFNEEYPDGWEGFSVKGLEEIGKYWGVLMGNPAETRSQAEL
                     FRKVLKNHIIEKGKHKDGIPDLAYLTIKATDAVAHQFGSESLEAKETFREADKQVGLL
                     FDFLQKEYGDKFIMVVTADHGGIPMPEISGGERYTIKELIHEVNSLLPESSQKTESLI
                     KFMAIAQISLDHDVMTKHSITEEMIIRKILSIQVNGKPFFKNVHRKSELN"
     gene            complement(7703..11383)
                     /gene="metH"
                     /locus_tag="PLF66_14055"
     CDS             complement(7703..11383)
                     /gene="metH"
                     /locus_tag="PLF66_14055"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004756335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HMZ65217.1"
                     /translation="MTTTKPNIEDLLKERILVLDGAMGTMIQRYSLTEEDFRGERFKD
                     HPHPLKGNNDLLCITRPDIIESIHLQFLEAGANIIETCTFSSSRISQADYKMEHIVRE
                     LNIAGVKVARSAIDKFQKTNPDHPCFLAGAFGPTTKTASLSPDVNNPAYRAVSFDELV
                     DVFYEQVDALLEAGVDLLLPETNIDTLNLKAAIFAIEKCFKNRGFRVPVSLSVTITDK
                     SGRTLSGQTVEAFWNSVAHAKPLSVGINCALGAEDMRPYIEELAHVAGTHISCYPNAG
                     LPNAFGGYDQTPEDFGHHLHDFAVQGWLNIAGGCCGTTPDHIRAVAEAVKNVKPHKPV
                     HPAEVTRLSGLEPLNITEDKGFLMVGERTNVTGSPKFKNLILAGDMEGALAVALQQVQ
                     NGANIIDVNFDEALLDGEKLMTEFLNLVASEPEISKVPIMIDSSKWSVIEAGLKCVQG
                     KAIVNSISLKEGEDKFIEQAKLVRLYGASVIVMAFDEQGQAATKADKVRICQRAYKLL
                     TEKVGFPPSDIIFDPNILTVATGIEEHNNYALDFIEATREIKATCPYAKVSGGVSNIS
                     FSFRGNNPVREAMHSVFLYHAIKAGMDMGIVNAGMLTVYEEIPKDLLELVEAVILNRR
                     PDATERLISFAESVKASGEKAQVKDDAWRKEPVAARLSHALVKGIVEYIDEDAEEARK
                     NYKKTLEVIEGPLMDGMKVVGELFGAGKMFLPQVVKSARVMKKAVAYLLPFMEAEKAS
                     SESNVKKDKILMATVKGDVHDIGKNIVGVVLACNNYEVIDLGVMVPADRILKEAREHN
                     VSAIGLSGLITPSLDEMVYVAKEMEREGFKIPLLIGGATTSPAHTAVKIAEQYSEPVV
                     HVLDASRVVNVMNELLNAEAKTKYAENVRKEQARLREQYLNRNAERNNLSLEDARKNK
                     PKYNWSTDEIAKPSFIGKRVFADFPLSDLVPYIDWSPFFHTWELRGRFPQILEDEVVG
                     KQATDLYNDAQKLLKDIVDNKRFTAKAIVGMFPANSVGDDIEVYSDESRSKILTTFHT
                     LRQQMTKPDGQFNYALADYIAPKDSGRIDYIGGFAVTSGHGADEFEKLFEEKHDDYNS
                     IMAKALADRLAEAFAEYMHKYIREEWGFGKDENLSNSDLIKEKYRGIRPAAGYPASPD
                     HTEKGILWDLLEVEKNTGIKLTESYAMWPGSSVSGLYFSHPDSKYFSVGKIGKDQISD
                     YAKRKNMEIQIVEKWLSPYLDY"
     gene            complement(11400..11699)
                     /locus_tag="PLF66_14060"
     CDS             complement(11400..11699)
                     /locus_tag="PLF66_14060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004788267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HMZ65218.1"
                     /translation="MAHVVLENCLDCKYTNCAAVCPAEAFREGPDMLYIDPDSCIDCG
                     ACIPECPVEAILPDYEIPNRSSSYIQLNAEESKKYPLIINNKRPLKGDRCIENKK"
     gene            complement(11740..12111)
                     /locus_tag="PLF66_14065"
     CDS             complement(11740..12111)
                     /locus_tag="PLF66_14065"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HMZ65219.1"
                     /translation="MRIIVDTNIWIDFLAGKPETLGLKSLVSELKVLRHIWIESELRV
                     GNIPKSEIFFSYYKQIPETIFVNYEILFQFIQKEKLAGKGLSFIDIGLYASAKMGNHK
                     IWTNDKNLKKLCESKKILFKG"
     gene            complement(12114..12308)
                     /locus_tag="PLF66_14070"
     CDS             complement(12114..12308)
                     /locus_tag="PLF66_14070"
                     /inference="COORDINATES: protein motif:HMM:NF021474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HMZ65220.1"
                     /translation="MRTTIILSETLINKAMEETGISEKTKLIHMGLELLIQQKAMEKL
                     SNLYGSAPKTKHITRGRKTN"
     gene            complement(12533..13846)
                     /gene="ahcY"
                     /locus_tag="PLF66_14075"
     CDS             complement(12533..13846)
                     /gene="ahcY"
                     /locus_tag="PLF66_14075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017855632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HMZ65221.1"
                     /translation="MSATTKTEDFKVKDLSLATWGREEIILAEKEMPGLMAIRKEYSS
                     KKPLAGARIAGSLHMTIQTAVLIETLTELGAEVRWASCNIFSTQDHAAAAIAKAGIPV
                     FAWKGETEEEYWWCTEQTIKFANGKGPNMILDDGGDLTYHIHTKHPHLLAEIKGVSEE
                     TTTGVVQLMKMMAKGELKIPAINVNDSVTKSKFDNLYGCRESLADGIKRATDVMLAGK
                     LCIVAGFGDVGKGSAASLRNFGARVIITEIDPICALQAAMEGYQVLRMEDIVDKADIV
                     VTATGNNDIVTLEHMKSMKDGAILCNIGHFDTEIQMARLNSMPGIQKKEIKPQVDKYT
                     FPDGKSIIILAEGRLVNLGCATGHPSFVMSNSFSNQVLAQIELYTNPGKYPVGVYRLP
                     KHLDEKVAAFHLEQIGVRLTKLTKEQAEYLGVSPEGPFKPEHYRY"
     gene            complement(13830..14822)
                     /locus_tag="PLF66_14080"
     CDS             complement(13830..14822)
                     /locus_tag="PLF66_14080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002975807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HMZ65222.1"
                     /translation="METISKIKNSNQGKMILSALKSASDETRIRILNILSFGSFNVNE
                     ITEVLGMGQSRISRHLKILHDAGLLQSQREGTWVYYKLPEDTTANPSFAVDMNRLILA
                     YKEDLPHRETDQQKVAILLRNREEKSTRYFNKVGKNWDRIQEEVLNPDLYRNQILSYL
                     PDKSETILDLGCGPGGLFPYLLKKSKQVIGIDSSEKMLEEARKTYSNQKKIKIVEAPL
                     ENLPFKTGTADAVVASMVLHHISNPPLVLNEASRVLKNKGVLCIVDLKKHDKEFMRDA
                     YADLWLGFDPNLLTDWLRAANFELKEIIEQETHSYFKIIAIKAIKKGGQNVRNN"
     gene            15044..17182
                     /locus_tag="PLF66_14085"
     CDS             15044..17182
                     /locus_tag="PLF66_14085"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HMZ65223.1"
                     /translation="MFSFFYPRQVLFLSFNCSVKRRLTFCALVFVLILGACSKTSSTI
                     KLAEKGVLDLSNWSCDENGSVRLDGEWEFYWGKYLEPNDFQSASLEKDYITVPHRWNQ
                     HKIGNDYLPADGYATYRLTIILPESNKVKAFRLSIQNTAYKLWIENDLIVETGKFGMT
                     KESMIPKYLPRTVEYYTDKRQITIILNVSNFHNRFGGIRTSIEFGSSENIKASRERSI
                     ALDLFVSGSLFIIGLYYIGIYLLRRKESSALFFAVYALMYSLKSLLEGERFLVHLYPD
                     FPWELDLKISYLVTFSAPAIFGSFLYSLFNAEFQKIVIRIIQFISFILVLIVLFTNAR
                     FYSILAIPHQIYLILVGLYASYIFIKAIKHSRAGAKILFIGFIVYFILAAINDSLYSS
                     GMIQSFYMSGYALFLFVLSQGLVLAIRFSNAFLSVERLTTELSKTNKAYSRFIPVEFL
                     KFLKKDKIQDVQLGDQIQEQMTILFADIRSFTTLSESMTPFENFNFINSYLKRINPII
                     RKNNGFIDKFIGDAVMAIFPHSAEDGVKAAIEIQNEMKVYNEHRRNNGYRPVEIGIGI
                     NTGLLMMGTVGDKNRMDTTVIGDTVNLSSRLESLTKEYRVPIIVSEHSVAKIPSLDQY
                     YLREIDVVQVKGKSKPVSIYECFATADSKEIENKTNSASLLMNGLIKFHLGEYEEALE
                     NFQKAQLISVDDPIIQMHIDRCKNLIQSKR"
     gene            17184..17909
                     /locus_tag="PLF66_14090"
     CDS             17184..17909
                     /locus_tag="PLF66_14090"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017213545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HMZ65224.1"
                     /translation="MLNKESNVKSYLVTGGSGGIGRAIVKDLAENYLVYNLDQKAPDA
                     KLKGEIFIRTDLTSSDSIQSALYSIKKEQLCGIVYSAGYGGPFVDITKVTEDLWDKVF
                     SINIRGAFILVKEFLSYFQQNRFGRIVVIASSQSIVGAKHSVAYSSSKHALIGFVKSI
                     ADEWGEYGITANAISPGYVDTAMGVQENEVSDHLKKILDKTPSKKIAKPEEISRIVKF
                     LISEESGYINGANIVVDGGITAI"
     gene            complement(17906..18859)
                     /gene="corA"
                     /locus_tag="PLF66_14095"
     CDS             complement(17906..18859)
                     /gene="corA"
                     /locus_tag="PLF66_14095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015679393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HMZ65225.1"
                     /translation="MFLSNWILTNKEKKEIKDTNIPLAIPKTKTWIHLTPKNEEELTR
                     LLQKFEIHSLTIEDIMNFHSRVKLEEFPNYTFLVFRGLHLENNRITAKNFNFVVTKNS
                     LITIVLDHRNTIRDLIVDWNKNKILLEKGIEFIIHKIIDVETDHIIPIVYRIEDQAEE
                     LESQVFAANTELDINSIFMMRGNLQQIKKVINLHIEILKELETRNTDFISVESDAFFR
                     DVRDHSIRILEIAETVKEMISSALEVHLTISTRKSNNIMKILTIMTAIMLPMSLIAGL
                     YGMNFKYMPFLEMEIGFYMTLIIMFLTGTMMALYFKLKNWF"
     gene            complement(18875..20617)
                     /locus_tag="PLF66_14100"
     CDS             complement(18875..20617)
                     /locus_tag="PLF66_14100"
                     /inference="COORDINATES: protein motif:HMM:NF036516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M61 family peptidase"
                     /protein_id="HMZ65226.1"
                     /translation="MAPKKKLPLKTNKTQLHYKVIITEPHLNYFHVEISFKSKNDKVI
                     FHMPTWSPGSYLIRDYSSHLHNFSVVANKGNPLKWKQIGLSSWEIISDSKPFTIKYRV
                     YAFENSVRTNFLDSEYGFINPPSLFLYPEGFLNNKIEISIDTNSYFKYIYSSLEKKEN
                     HYIASNFDQLYDSPIHISNHESISFETGDCHHELIIEGNISKQIKANLISDLKKITDY
                     ETKLFGINPNKYYLFILNLSDDVYGGLEHASSSVNAFDPSKLHERNEYNRLLGLLAHE
                     YFHLWNVKRIRPIELLPYNYLEPNLTRELWIAEGITSFYDNYILHQCGFYSNEDYLAE
                     LLSDISRLEDSIGDENMSLEDSSFTAWTKFYKQQPNSHNTGISYYIKGSILVLCMNIY
                     ILKNTDCKYSFIDILKALYHKFYIKKNRGFSKEEFFQTSEEVLGFNLQDKFEKYLSDT
                     IRIPVFDFLEEVGIKREEVNSKISFGFDTKERDGKILISKIYQHKTAGKTDINLNDEL
                     IAINGQRASREMIESTKASLETGDEITLLLSRKNKIIERKLKCETSRTYRLVFDNVPS
                     EKILKLRKAFLGNR"
     gene            complement(20712..20912)
                     /locus_tag="PLF66_14105"
     CDS             complement(20712..20912)
                     /locus_tag="PLF66_14105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_465540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HMZ65227.1"
                     /translation="MAQGTVKWFNKVKGYGFISIDGGEDIFVHYSEILKDGFKQLKDG
                     ERVEFEIINGKKGPAAAQVKAI"
     gene            complement(21150..21485)
                     /locus_tag="PLF66_14110"
     CDS             complement(21150..21485)
                     /locus_tag="PLF66_14110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010573885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65228.1"
                     /translation="MIHHLAIATTNVLKMAEFYKTLPGLNWKEDKFTVDGSLRSVWFD
                     TANGCLLMLEKFPYSKAPEALIFQFEPHFNFTDIGLTIIKRTDYTFYFNDPDGNQLGY
                     SSYPNKLKF"
     gene            complement(21591..22070)
                     /locus_tag="PLF66_14115"
     CDS             complement(21591..22070)
                     /locus_tag="PLF66_14115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002075466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2147 domain-containing protein"
                     /protein_id="HMZ65229.1"
                     /translation="MKKKFLAVAAFVAIFFAGSLSANDKILGKWLTEGGKSHVELSKC
                     GDAVCGKIVWLKFPTYAESDAAVKDGKAKAGDAKVDTENPDASKRTQPIVGLVFLKGF
                     KYDAGDDEWTNGEIYDPESGKLYVGELKMDGANSLKLNGHLKISKWLGKKQTWTRVQ"
     gene            complement(22232..22483)
                     /locus_tag="PLF66_14120"
     CDS             complement(22232..22483)
                     /locus_tag="PLF66_14120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65230.1"
                     /translation="MNRDEKVIEIESELCQLKLCVDSEDLESMKRKELANSLLHKIDS
                     DLLTIQMDSFQHSKFNIGVFHLYIELEILRNSLKKTGIL"
     gene            22742..24277
                     /locus_tag="PLF66_14125"
     CDS             22742..24277
                     /locus_tag="PLF66_14125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65231.1"
                     /translation="MSQNNLKIQILWRVGLFIISILFLDWFFFSYLFLKIPNELEWDT
                     SPWYNFLQKKETISFPENKKKVLVTGSSVALYSYLPQDVNLDLVNASIQSDFYSHVAM
                     SPTDFYYYADSIIAKKPDLVLYLINPGDLQMDHFPKAKDGQGFLDYSEEGRLHAYAER
                     HPVKFYYPLNFLMENLSSLRKIEILHLLTKSILYVNRYRSFAFDPIDAYLERHTRRKT
                     SYHNYTGVTLNPIQWRKGWTSQKFEVECETKKDNFFSDTIFIPEKDTTVKVFAANGDL
                     LANVSYTKAGWHILEFNFSSGQNKFHFEVSKVVSSKLIDNKLYGKEYFYGIRLSQNFC
                     KNELEQNIAYNRSNSQEDEILKDMSNVDYETDYFTRMYKDADQYHDLEKKIPVRPEVQ
                     RLYHLHNVKKYLNQKGAYRWSEFSMLERAAKKMQDAKIKFVIINNPENPLELNLYKDS
                     IWYKDYLNFFQEMSQNYSIKFYDHKESNLSIQDFVDSHHLTYGGSKKMTKIYSEIIKD
                     NIR"
     gene            24281..25480
                     /locus_tag="PLF66_14130"
     CDS             24281..25480
                     /locus_tag="PLF66_14130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015679281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65232.1"
                     /translation="MNTVINFLEKSYIRYSLFLIVYSIVTISLWQKYNYNPTSMINFG
                     MEFAVQNKEKMPDGAVVILGNKDDLGAGYDGQIFYFYSRTISELNMNWPLGFDESYRA
                     PRIGYPLLVGIIGFFGKMGAVYGMYIVNISLFLLSYILLRRILDEDKKIYSIFYLLSP
                     FSLGSYAVLVSDSVMVSLVIIAYYFYQEEESLYFVLVASLAILCKEPALFLFFPLGVK
                     ALIDKRIPKALLVLSILVIPILWHLYLKVTFPNWRATRLTDFILPLEGIITYTKSILN
                     SVSNTTDYKELARLLSRFPLLVLLMVGLITLFTGNIKRGIEFRLGIGLVFFMVSTASY
                     YHFWSVYENVSRMFTLSIPLFILLKNDEEDTKTGLYFLLTILILLLFLVKVVLIQKTQ
                     SYTIWGM"
     gene            25487..26176
                     /locus_tag="PLF66_14135"
     CDS             25487..26176
                     /locus_tag="PLF66_14135"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HMZ65233.1"
                     /translation="MHKKKIIVVGAGSGIGKALFERVRKTNDSDVYGLSRRGESFSNK
                     FQSGLNYRCDVTNEGDIHNFFYKYQESNKTLDALFLCQGDGLFGEIEKIDRLSLEKHF
                     QLNLFSQFSILQFAYPLLRISDHPFVCFLSSTAGKVGFPESTAYCASKHAVAGLAKSL
                     REEWKKDNIRVMTVYPGAISTSIWEGREGFDQNDMIAPEDFAEYLASFITLPKTINLD
                     ESYVLPQKGIL"
     gene            26176..26901
                     /locus_tag="PLF66_14140"
     CDS             26176..26901
                     /locus_tag="PLF66_14140"
                     /inference="COORDINATES: protein motif:HMM:NF014626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HMZ65234.1"
                     /translation="MCTRFSLVFNQLLEIQEQFNTHNVLDGMDFKKMYLPTDRILVIY
                     RNEELGKNEMIKMRWGLIPNFIKDFDQIKKYSLYNARSESLEEKQSFKSLLNRNRCLI
                     PVDLFYEYKTDGEVKEQYAFSLNPESRFAVAGLWDLWTNPKTKEKIYSCTMITTIGNE
                     IVAPIHEKNRMPVILERDLYDKWLDPNIDFSVLKDLIVPYNASKMKAVKNTDKDNLPL
                     NPRIKISNQEKEELNQPSLFSSK"
     gene            26979..27665
                     /locus_tag="PLF66_14145"
     CDS             26979..27665
                     /locus_tag="PLF66_14145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HMZ65235.1"
                     /translation="MELSILFSILTQCVPQIYKDSPGFIGLKDRSKTKTEYFPENAKY
                     LGFKENFNPDLNQFRQFSNLESLEISSPELKDLSQLGSLKTIQYLNLSGTSVENLMPI
                     SELSNLHSLILNQTLVSDLSSISKLKKITRLSLANTKIKNIEFCEYFPELRRLDIRNT
                     LVEDLKPIMKSEKLIDLYIGGTKIQNLKPLYGLKDLVYLEVDSTSIAEKDVLDLRKVL
                     PYLKVVQRGF"
     gene            complement(27662..28318)
                     /locus_tag="PLF66_14150"
     CDS             complement(27662..28318)
                     /locus_tag="PLF66_14150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65236.1"
                     /translation="MKLNKTIFLILIFPICLSASDFIPAKIWFSNKLEFTNGFEFIQL
                     FIQQDRFILAKLNYNKIRKWEELRIIGTIKTNSENEIDFYPEMCSVFATKKLGLRWIL
                     IQGFDCDHLNIKFTKNRQEISICPSIGILENYTIKSDSKPSENSISAIVVSKEENISQ
                     AWSLQMRYIKKGSNGLYKNIPFQVLETVDSTGSFVSSEFIPIGELIQISNPKQTGFFD
                     "
     gene            28421..29941
                     /locus_tag="PLF66_14155"
     CDS             28421..29941
                     /locus_tag="PLF66_14155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase MA family protein"
                     /protein_id="HMZ65237.1"
                     /translation="MRSTLTLLFQTFFLLIFIIQFNGCYTQSKSRDSKNIMSILSLKL
                     VIDAQNSGCNGGFWVRNLLSSTQASYCVNAKLVSSTTNMDLYLDNNLTTNLNYSNITN
                     AFETNIFPIERDAYGSPSDINGDKKVTVLILDIKDGSSANTGFIAGYVDPINFYIDSP
                     GSGLRSNQREILFMDGVELVALRDRDLANGKPDTFLSTLAHEFQHLIRFQYSRGTDDT
                     WIDEGTSEVTSDLTGYGPQSARFSCFKGDANSSSACLGGIGSTSLGSPSLFNWSGTLK
                     NYAYAYTFMKYIYESSGSNSTSRNTFFRQTVQGNNGVRGNTAYNLISLFMGSSNFNAN
                     VLNSDNKTTFKMLMASFLGQAVGYSNLNNVYFGNTTPVNIDSVRTTYPFSSTLTLLSS
                     PNPFSGINTSSFNLSPSQVSRVIKSTTGVTSNSSDIVIVSNGSSGANTEFVIFNGDNV
                     GSRTATTSATASTLELISYPELDLKDTDGIICPNSHFSKLNEIDQKRLDLKLYQKK"
     gene            29954..30652
                     /locus_tag="PLF66_14160"
     CDS             29954..30652
                     /locus_tag="PLF66_14160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65238.1"
                     /translation="MSDKYRYALLVIVSSLIGVSFEVFAQENYTFLNRAADRFQKEKV
                     ELKNYRVKQEVASKIKETSGSVIEKRFQIGYFRSPDEFIFICRELEINGMKQFLNKPL
                     IERTSKTEWDWLSKEGISLHSFQILSIESNAVKYLVSPKKILTGFFRGQIWIQPDSGR
                     ILKIIKEPIIKKKEMMQYMIEVNFDKDYRFQMPSRTILRAVYQVGSRITEVEVDARFQ
                     DYEFNIDFSQEIPK"
     gene            30841..>31721
                     /locus_tag="PLF66_14165"
     CDS             30841..>31721
                     /locus_tag="PLF66_14165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65239.1"
                     /translation="MTQNFVKFIPIGDTGSICYLFDNLKDFEGKEMDQLQSLISDWKN
                     RKIQRMILLKSLTKKIDYDSVTNRLSTNGFSSILYSPSQDLKTQVQIFETLLDKDLKG
                     NIGVLFLGSSPSLIELIVKISIYADKNTSASEVYQYLTDEEPAELTLEELKKFQDFLE
                     TDPYILTKPDRAEFKRLTPPIFEKIETKSALSAEEANATTAFTLNLSSLNTNLSDDAP
                     LLSETSRIQFNLESLVETDFRKEESPEEHYENLKTTTMPQEIDDSLSLDVDLAEEIEY
                     ISSLESLPSNPTLQKIS"
BASE COUNT        10026 a         5145 c         5507 g        11043 t
ORIGIN      
        1 ggcaatgtga acaacaacaa taagactaat aacaattatg tgcgttgtgt gtcgggagcg
       61 tggtcatgat ttcgtcccct tctaattttt tttcagtaga gaatttgcat aaggcatact
      121 taaagtgtag gaaaggaaaa cggggaaaaa tccatacaag agggtttgag attgagttgt
      181 accagagact caatgaattg tctctctctc ttcaaaagca aagctataag ccctctcgtt
      241 ctgtttgctt ttatgtatct aaaccaaaac tgcgggaaat ctttgccgca gattttggag
      301 acagggtagt gcatcgacta ctcattgact taatagagcc ttattatgaa aagaaattta
      361 ttacagattc gtttgcctgt agaaaagaaa aaggtactca tagagcagtc ctacggctaa
      421 aagagtttat ccgcatagga acaaatcatt ctaaaaaacc tctgtattat ttacagttag
      481 atataaaagg tttttttatg gaaatagata aacgaatatt atgctctatt atagagcctg
      541 agattgacca aactttacag tggttgtgta aaacgattat ttgccacaat cctacaaaga
      601 agtttattta tagaggaaag gtcgttaaaa aagggatatt gcctcctcac aaaactttgt
      661 ttcatgaaga cgagaacaaa ggaattccaa taggaaattt aacaagtcaa ttctttgcga
      721 atgtttattt gaatgaacta gaccagttta taaaaagagg actagggata aaatactata
      781 tcagatatgt agatgacttt ttaattttgc atgaaaatcc agagaagtta atgcattgga
      841 aagaaaaaat tatacaatat ctatatcaaa aattgaaact tactttgaag gatgatgtta
      901 tgcctttatc agtatacaaa ggaattgatt tcctaagaat tccaagagtt ttatctcgtt
      961 tttctagatt tcgattccag attcgatact atatgaaaga attttctaaa catcttttga
     1021 tagtccaagt gggaaaattt tttgaattgt atggagctgg cttagaagag gttattccct
     1081 atttgccttt taaaccccgt tatagaggac aaatgaaaac gtttggaata ccaataaaga
     1141 aaagcaaata tgcaaaaaat tatttgaaag atattttaaa aatatgtaaa cgaaaatata
     1201 taacatatct aattttaact caatcggatg agcaagcgga atctgtaaaa attcgaatgc
     1261 ctcggtatca gcaacaatta atccacccca tacaactgga attatttaat aattcgcaaa
     1321 aattttataa atataggaga aacaaaaatg tttaaaaaaa aaataattag caatgtttcg
     1381 tttacgctac tattacttct acttgcaaag caaatagccg ctactccctt tacagataat
     1441 ggagatggga cgattaagga tagtgcttca gggcttgtat ggcagaagtg tagcagtggg
     1501 caaaatgcga ctacctgctc tggaacggcg acggctctaa catgggcaac tgcaattact
     1561 gcgtgtaatt ctctaagcct tgcaagtaaa acttggagac tgccaaatcg aaatgaattg
     1621 caaagtatcg ttgattatac aaaaacctca gcgccaatga ttgatgcaac agcctttcct
     1681 tctactcagt cctcttattg gagttctact acctacgcgc cttttactag ctttgcgtgg
     1741 tacgtctatt tctctgttgg caatgtgctc agcaacttta agactgatat ctattatgtg
     1801 cgttgtgtgt cgggaccgtg acttggttat tgggtcattt ggttattttc ttttagtttg
     1861 atttttaatc gaaatattta cgttatgcga aagtgtattt gtttaaaatt taatttagcg
     1921 aataatgcgg attattcgga tgtatgagaa accgcgaatt tcatcattaa atgaaggcga
     1981 cgtcgcgtga cgtcgccttc aatcattcaa ccgcaccgaa attctcaaaa acgaatatat
     2041 attcaaagtg atttattttg tgagactttc ttgttggatt tacattttac ccaaaaatta
     2101 aaaaaccttg caatattggg ttacaagatt aagaggataa aaagaattca ataaattgct
     2161 tgccttctta tttcttctag ctatagtaac cgtatggaag cctctaaatt acaaattaac
     2221 tataaagaat tactttttaa aatttcttct tttccagaag agacgactcg acaattatta
     2281 gagtatattg attttctttg ggaaagacat agtcctgctg tggacgggtt atcggaatta
     2341 gataaaaatg aattagacaa aagagttaaa aaatattttc aaaaccccga aacaggtttt
     2401 tctcttagtc aagttatcag tagactagaa aaagatttag gagaaaaatt aagttagtcc
     2461 atgcatcagg caatttttac agcagaggct aatcaggaat tgcgggaagc aatactttgg
     2521 tttgaaaatc agtagaaggg tgttggaaaa aatttagcca atgaggttat agataatgtt
     2581 cgtaagttag aagcaaaacc agatgcatat tcttccgatg ataaaggtct acgcaagacc
     2641 ggtttgaaat ctttccctta ttttatctat tatcttttca aatcgccaat agtgttaatt
     2701 attgcagtct ggcataagag tagattaacg gatgctgttg aagaaagggc tagtaaaatc
     2761 attgttgata aggatgaaag ctgaaaatat atttccctgg caaagaatat actagaagtt
     2821 tggaaagaaa tttgcaagta aaatttattt agtgaataat gaggattatt caaaagaagt
     2881 atgattgaaa gtgaggtcac gtgaccgcgc attcaatttt aagcgcaaaa taaaaattac
     2941 ccgcactaga acggttttct aatcctcaca aaattatatt caattacgct gaatgaaaaa
     3001 cgcatttgct ctagattatc ttggattgta tttaacacaa tggatgcttt ggtttcactt
     3061 ggtaaaccgc ttaggcgaat taggatgcgt caacactttc atcagcgaca aagtgcatta
     3121 tgccgccgaa taaactactt cattttttat cagaagcgaa gcgtaaagca aacaggcatt
     3181 aattgagact tgattaattt tggggtaggc taatagtaat tgttcgatcg tttcaccaag
     3241 tgccagtttt tcaagaatta agtctactgg aattcgcgtt ccctttataa ctggcttgcc
     3301 aaacataatt ttagggtctc ttgtaatata ttctttaacg tccatcgtaa taattagtat
     3361 gttgacaatt gaaggaaaaa agcaagtcaa attagataag cggaaactaa aagaaaatac
     3421 tagcagaatg cttagcgttc taaataaaac gaactccaaa aaaagattga ttcttgttgc
     3481 atagaactta tattatagat aggactgatt taattataca aattccagag actgcatttt
     3541 ctatcacccg ctcagataaa gaatcttttg ccaaagaact attgcttgca gctgcaatga
     3601 aatggtatga gatgggtaaa atttcatctg gaaaagcagc taatttggtt ggtatgaacc
     3661 gagttgattt tttatatagg ctcaaagaat ataatatttc tacctttcaa tatacagcag
     3721 aagaaatttt agaagagtaa aaattcctgc atgcctaatt ttatttcaaa tacatcgcct
     3781 attctttatt tatccaagat agaacttatt gagaaaaatt actcaccttc tgaaaatttt
     3841 attattccga atggagtgaa agaggaaatt ctatcaggca ctgtaaacga ttccgctaaa
     3901 caatggatac aaaatcctga aaataaatat tgttatgtaa aaaattaacg actacgtttc
     3961 aacgtaagaa gctaaactcg caattaaacc ttgcttgatt ttaaaattaa gcaaggttaa
     4021 aaggaagggc ggaggctttt tatgcaaaca atattgattc aggttacgaa tgaaaaagca
     4081 tacaagatac tagctgattt agaagattta aatttattaa aaatcttaaa acaaatggat
     4141 actataccca aaaagctttc ccaaaaatat gcaggtaaat tagattcaac tattatagat
     4201 gagtttcaaa aatttgttca aaatggtagg aatgaatgga acaacgcaag tatctaattt
     4261 aaatgtttac gctctcgatg atgaagttgt tgctgctatc attaaaattc gtaaaaatta
     4321 caagacgaag tagccagatg ctttgattgc agctactgca ttggtaaacg attttatttt
     4381 aattactcga aatacttctg attttaaaaa tatttctcac ttaaacattg ttgatgcata
     4441 taatttatag taaaaaattt aatttagcga ataatgaaga ttattcggaa atgtatattc
     4501 atgggaaacc ccaaaagtca tcgttaaatg aaggcgacgt cacataacgt tgcttttatt
     4561 tataaaacat tccccaagat gcccatagca gaatgatgat aaaatgagtg gtattagtat
     4621 ggtctcattg cgtttctgtg cctatcgtcc tcccttccaa aatccattgc tgtttgattg
     4681 cggaaatcgg aggcaatcat ttataaaaac tcctacttcc cactcacttc taattcttta
     4741 atgtattccg tgatataaag gattttcttt tttacctgtc catctaggta gattagattt
     4801 ttagatggaa tttgagaata gaaggagaga gcttttttga aggaggctct tgctagatct
     4861 ggattatggc tgatgatata aagttctgca attaataaat agacttctcc tttgaagagg
     4921 ggagagagtt ttgcttcgtg ttcattcatc ttggtttgga aaaattctag aaacttttct
     4981 tttaataaaa agtcgacatc tgatagtttt atattttcga ttaagtttaa aagaggaacc
     5041 acatcaaaaa aatcataagc ggaatagagt tctaaagttt ggaaaaaata tttcttaaaa
     5101 tttggaatag tttgcattcc aaaaaatgca gagattagat taaaatagga cggagcaaag
     5161 attttatttt tctcaatgct attgtatgca tattgaatac tttcctgata ctttgcattt
     5221 gttagagcat tcgcactatc taaataaaat ttataataat cttctttgat gatatgtgtt
     5281 gatcgatctt tttgatttac attcatcagc cagtcaaaca tataaaaagc ttcttcgggt
     5341 gttacctcat gtcgcattcc aggatagact tttcgaattg catatggatt gtaacgtttc
     5401 aaaacctgga agcgattatc catttctctt ttggtgaatg cttctccttc tcctgtatct
     5461 ccagaaataa atagaatatt tatttttcca gcagtagaaa tttgtttatc ggttaacgat
     5521 gtgtaacccc cagataatcc tgcaaaatat ctaaatatat caggatttgt taatgctgtt
     5581 tgtagacctt gatttccgcc ttgcgaaaat ccaaagagaa atacatcatc atacactttg
     5641 tattttgttt tgatgagttc tactaagttt agaatgtatt tatcactttt atcgtatccc
     5701 catttcactc catttcttcc attgtcagta aaatttccat cagggcatac gaggataatg
     5761 ttcatatacc gcgaatagta gctaaatggg tttaccattc tactaccatc gctgaataat
     5821 ccgtgcaatc ctatcgctaa aatatacttt ttgttcttat tataatttac cggtaaaacg
     5881 gttgaataag aaattccatc ttcatatgtg aaaatattgt ctagtttgat ttgagcaaaa
     5941 gactcattcg ttattataaa tataaaaaat aaaaaaatct taaaataaag cttcattcaa
     6001 acaattaatt tagttctgat tttctatgca catttttgaa aaaaggcttt ccatttactt
     6061 ggatacttaa gatctttcga atgatcattt cttctgtgat agaatgcttt gtcattacat
     6121 catgatctaa agaaatttgt gcaattgcca taaatttaat taagctttca gtcttctggg
     6181 aagattctgg tagtaatgaa tttacttcat gaatcaactc tttgatcgta taacgttctc
     6241 caccagagat ttcaggcatt ggtatccctc cgtgatctgc ggtaacaacc ataataaatt
     6301 tatctccata ctctttttgt aaaaaatcaa agaggagacc aacttgttta tctgcctctc
     6361 taaaagtttc ctttgcttct aatgactcag atccaaattg atgggctaca gcgtctgtcg
     6421 ctttaatcgt gagataagct aggtcaggaa ttccgtcttt atgtttgcct ttttctataa
     6481 tatggttttt taaaaccttg cgaaataact ctgcttgtga tcttgtttcc gcaggatttc
     6541 ccattaatac tccccaatac tttccaattt cttctaagcc ttttactgaa aatccttccc
     6601 atccatctgg atattcttca ttgaaagttt tgagtggatt gtattctgct gtctccgatg
     6661 gaagcgaata atatctatta tccgttaccc atttggactg agaagtcgcg agccaataga
     6721 ctaaatcttt gtccccaata tagtctacag atttaatctt tgccgctcca tgaccagcca
     6781 ttccaatgga agcacgaagt gcatagcatt gactaactac ttctgattct ccgtgatatt
     6841 cattgtctag aacatctgct aacgtttcag tccaaagttc ttccgggttc actgtgtctt
     6901 ctgttagtgt atagatttca tctttttcaa actttccgtt ggaaaatcgt aagtaaccat
     6961 ttgcaatgat tccatttttt ctaggataag ctcctgttcc aatggcggca tgacctacag
     7021 cggtttctgc atcgatatgc gcgacatgag catttggaaa ataagcggat tctcttttga
     7081 gtttagaaat ctgaatcgga atctcaggat gtgctttgta atactgctgt cctgcttgat
     7141 ctaagacaac agttacaatg atttcaggta aatttgcatt tgttcctgtt tttaaaattt
     7201 gattgagtga ggatgtttct actccatttg gatttctaat ttttagaatt tttgcgagag
     7261 taggaactat gtgttgttga tgaatcggtt ctgaatattc ccctttttga atccaatttg
     7321 gaccataaaa gatgactgga atacttgaat catattcata gtgagtagaa tgagctgttc
     7381 ttcctgctaa aattgttact ttattgtcag attcggtgta ttgtaactca ggattgtttt
     7441 ttaagtcgtc tatcgttcta ccataggtct tgagatgacg tatcaaatcg gatttatcat
     7501 aacgaacggt atgtttaatt tttatccaat cttgctctaa aacattgaat ttatttggag
     7561 ttaaaaataa atcaatgtct ttagtggcag agcgtttaat ccattctaac tctaagtttt
     7621 tcttgcagct aacaagggag agtagaatgg ataggaatat aaaatttctg aagtagttca
     7681 cgtatcactc ctttagagag acttaataat ctaaataagg agagagccac ttttctacaa
     7741 tctgaatttc catatttttt cgttttgcat aatcactgat ttgatcttta cctatttttc
     7801 ccacagaaaa atatttagaa tctggatgag aaaaataaag tccactgact gaacttccag
     7861 gccacattgc ataactttcg gtgagtttaa tgccagtatt tttttccact tctaataagt
     7921 cccaaaggat tcctttttcg gtgtgatcag ggcttgcagg atatccagca gcaggtctaa
     7981 tgcctctgta tttttctttg attaaatctg aattggaaag gttttcatct tttccaaaac
     8041 cccattcttc gcgtatatac ttatgcatat actcagcaaa tgcttctgcc aatctgtctg
     8101 ctaacgcctt tgccatgatt gagttataat catcatgctt ttcttcgaat aatttttcaa
     8161 actcatctgc accatgtcca gatgttacgg caaaacctcc aatataatca atacgtcctg
     8221 agtcttttgg agcaatgtag tcggctaacg cataattgaa ctgtccatca ggtttagtca
     8281 tttgctggcg aagtgtatgg aatgtagtta ggatcttaga tctggattca tccgaataga
     8341 cttcaatatc atcgcctaca ctattagcag gaaacattcc tactattgct tttgcagtga
     8401 atcgtttatt gtctacgatg tcttttaata atttttgagc atcattgtaa agatcggtag
     8461 cttgttttcc tacaacttca tcttccagta tctgtgggaa tcgacctcta agttcccatg
     8521 tgtgaaagaa aggagaccag tcgatgtaag gaaccaaatc agaaagtggg aagtcggcaa
     8581 atacgcgttt gcctataaaa gatggcttgg ctatttcgtc tgttgaccaa ttgtattttg
     8641 gtttattttt tcttgcatct tctagactta aattatttct ctctgcattt ctgtttaaat
     8701 attgttctct aagtctagct tgttcctttc ttacgttttc tgcatacttt gtttttgctt
     8761 ctgcatttag tagctcattc attacattta caactcgaga agcatctaaa acgtgaacga
     8821 caggttctga gtattgctct gcaattttca cagcagtatg tgcagggctt gtggttgcgc
     8881 caccaatcaa aagtggaatt ttaaatcctt cccgttccat ttcttttgca acatagacca
     8941 tttcatcaag agaaggggtg attaatccag aaagtccaat agcactaacg ttatgctctc
     9001 tagcctcttt taatattcta tctgctggaa ccataacgcc caaatcgatt acttcataat
     9061 tattgcaagc aagcacgaca cctacaatat tttttccaat atcgtgcaca tcgcctttaa
     9121 cagttgccat tagaatttta tcttttttga cattggattc actggatgct ttttctgctt
     9181 ccataaatgg gagtaggtag gcaacggctt ttttcattac acgtgcactt tttacaactt
     9241 gtggtaaaaa cattttacca gcaccgaaaa gttctccgac tactttcatc ccatccatca
     9301 aaggaccttc gattacttca agagtttttt tgtaattctt tcttgcctct tctgcatcct
     9361 catcgatgta ttctacaatt cctttcacaa gagcgtgaga aagtcttgcg gctaccggtt
     9421 cttttctcca agcatcgtct tttacttgtg ctttctctcc actcgctttg actgactcgg
     9481 caaaactgat taatctttct gtggcatccg gtctacggtt taggattaca gcttctacaa
     9541 gttccaataa atcttttgga atttcttcat aaactgtgag cattcccgca ttaacaattc
     9601 ccatgtccat accagctttg attgcatgat ataaaaagac agagtgcatt gcttctctca
     9661 caggattatt tcctctaaaa gaaaaagaaa tattgcttac tccaccactt acttttgcat
     9721 aaggacaagt tgcctttatt tctctagttg cttcgatgaa gtctaacgca taattattat
     9781 gttcttcgat accagttgcc acagttaaaa tatttggatc aaaaattata tcactaggtg
     9841 gaaagcctac tttttcagtc aatagtttat aggctctctg acaaattctg actttgtctg
     9901 ctttggttgc tgcctgacct tgttcgtcaa atgccattac aattacactt gccccataga
     9961 gacgaactag ttttgcttgt tcgataaatt tatcttcgcc ttctttgaga gaaattgagt
    10021 taacgattgc ttttccttgc acacacttca aacctgcttc aatgactgac catttagaac
    10081 tatcgatcat gatgggaact ttcgaaatct caggctctga tgccacaaga tttaaaaact
    10141 cagtcatcaa cttctctcca tcgagtaagg cttcatcaaa atttacatcg ataatatttg
    10201 ctccattttg cacttgttgt aaggctactg caagtgctcc ttccatatct cctgcaagaa
    10261 ttaggttttt aaattttgga gagcctgtga cgttagttct ctctccaacc attaggaatc
    10321 ctttatcttc tgtgatattt aatggctcta agcctgaaag tctggtgact tctgccggat
    10381 gaacaggttt atgcggttta acattcttaa ctgcttctgc tacggcgcga atatgatccg
    10441 gagtagttcc acaacaacct cctgcaatat tcaaccatcc ttgcactgca aagtcatgta
    10501 aatgatggcc aaaatcttca ggcgtttgat cgtatccacc aaaagcattt ggaagacctg
    10561 catttggata acaactaata tgagttcctg caacgtgtgc tagttcttcg atatatggtc
    10621 tcatatcttc tgcgcctaac gcacagttaa taccaacact gagtggcttt gcatgggcaa
    10681 ccgaattcca aaacgcttct actgtttgcc cagaaagagt tcttcctgat ttatctgtga
    10741 ttgtaacaga gagggaaaca ggaactcgaa atcctctgtt tttaaaacac ttctctatag
    10801 caaaaattgc tgcttttaag tttaaagtat caatatttgt ttctggtaag agtaaatcaa
    10861 ctcctgcttc taagagagca tctacttgct cataaaaaac atctactaat tcatcaaagg
    10921 aaacagctcg ataagctgga ttattgacgt caggcgagag agatgcggtc tttgtagtag
    10981 gtccaaaagc gcctgctaaa aagcaaggat ggtctggatt tgttttctga aatttatcaa
    11041 tagcagatct tgctactttt acaccagcaa tatttaactc tcgaacgata tgctccattt
    11101 tataatcagc ttgtgaaatt ctagaggaac taaaagtgca agtttcaatt atattcgctc
    11161 cagcttctaa aaactgtaga tgaatgcttt cgataatatc tggtctagtg atacagagta
    11221 ggtcgttgtt tccttttaat ggatgaggat ggtctttgaa tctttctcct ctaaagtctt
    11281 cttcggtcaa tgaatacctt tgtatcattg ttcccatagc tccatctaga actaaaattc
    11341 tttcttttag taagtcttct atatttggtt ttgtagtagt catctgaatg tttgccgcat
    11401 tattttttat tttctatgca gcgatctcct ttgagtggtc ttttattatt tataattaat
    11461 ggatattttt tggattcttc tgcgtttaac tgaatgtaac tgctagatct atttggaatt
    11521 tcataatcag gtaaaattgc ttccactgga cactctggaa tacaggctcc acaatcaatg
    11581 caggaatctg gatcgatata gagcatatcg ggtccttctc taaatgcttc cgcaggacaa
    11641 actgcagcgc aatttgtata tttacaatct aaacaatttt ctaatacgac atgtgccata
    11701 tctatattcc ttttgtattt gatagaaatt tcatggatgc tagcctttaa atagaatctt
    11761 ttttgattcg catagttttt ttagattctt gtcatttgtc caaattttat gatttcccat
    11821 tttcgcagag gcgtagagtc ctatgtcaat aaagctcaaa ccttttccgg ctagtttttc
    11881 tttttggatg aattggaaaa gaatttcata gttcacaaaa attgtttctg gaatttgttt
    11941 gtagtagcta aaaaatattt ctgatttagg aatatttcct acacgtaatt ctgattctat
    12001 ccagatatgt cgtaatactt ttaattctga gacaagagat ttgagtccca atgtttcagg
    12061 cttaccagct aaaaaatcaa tccagatatt tgtatctaca atgattctca ctgctagttt
    12121 gtttttcttc ctcgtgtgat atgttttgtt ttcggagcag agccataaag gttagagagc
    12181 ttttccattg ccttttgttg gattaacaac tcaagaccca tgtgtatgag ctttgttttt
    12241 tcactaattc ctgtttcttc cattgctttg tttattaatg tttcagaaag tataatagtc
    12301 gttctcatgt tctgacacct caaattgatg catataaaca gtatatatga atatgcatca
    12361 gtttgcaagt agaatactta aagattgtcg ccgattcagg cgatgaacgg ttcgactaga
    12421 cttagttggt gagcttgtcg aatccacacc gcatctcacc aataaaagat gcgatcattt
    12481 aaagctaacc atcgttcagt tatcggtgtg catcggtggc aatccttaat ttttagtatc
    12541 tgtaatgttc tggtttaaat ggaccttctg gtgatacacc taaatactca gcttgttctt
    12601 ttgtgagttt agtgagtcta acaccgattt gctccaaatg gaatgcagca actttctcat
    12661 ctaagtgctt tggtaaacga tatacgccaa ccggatattt tcctgggttg gtatagagtt
    12721 caatttgagc aagaacttgg ttagagaaag aattagacat tacaaaagat gggtgacctg
    12781 tagcacaacc aaggtttact aagcgaccct ctgcaagtat gataattgat tttccgtctg
    12841 ggaaagtata tttatccacc tgaggtttaa tttctttctt ttgaattcct ggcattgagt
    12901 taagacgagc catttgaatt tcagtatcga agtgtccaat gttacaaagg attgctccgt
    12961 ctttcatgga tttcatatgt tcaagagtaa caatgtcgtt atttccagtt gcagtaacaa
    13021 cgatatccgc tttatcaacg atgtcttcca ttctaagaac ttggtatcct tccatagcag
    13081 cttgaagagc acaaattgga tcgatctcag tgatgattac tcttgctcca aaattacgaa
    13141 gagaagccgc agaacctttt cctacatcac caaaacctgc aacgatacaa agttttccag
    13201 ctaacataac gtcagttgct cttttgattc catctgcgag agattcacga catccgtaaa
    13261 gattgtcaaa tttagatttt gtaacagagt catttacatt gatagcaggg atttttaatt
    13321 cacctttagc catcattttc atgagttgaa caactcctgt agttgtttct tcagaaacac
    13381 ctttgatctc agcaagtagg tgtgggtgct tggtatgaat atggtaggtt aaatcgccac
    13441 catcatctaa gatcatattt ggaccttttc cgtttgcaaa cttaattgtt tgctctgtgc
    13501 accaccagta ttcttcttca gtttcgcctt tccatgcaaa tactggaatg ccagcttttg
    13561 caatagctgc ggctgcgtga tcttgagtag agaaaatatt gcaagatgcc catcttactt
    13621 ctgcacctaa ttcggttaac gtttcaatca atactgctgt ctgaattgtc atgtgcaaag
    13681 aaccggctat tcttgctccg gctaatggct ttttgctgct atattcttta cgaattgcca
    13741 tgagacctgg catttctttt tctgctaaaa tgatctcttc tcttccccat gtagcaagag
    13801 ataaatcttt tactttgaaa tcttctgttt tagttgttgc ggacattttg tcctcctttt
    13861 ttgattgctt tgattgctat aattttaaaa tatgaatgcg tttcttgttc tattatttct
    13921 tttaactcaa agtttgctgc gcgtagccag tcagttaata aatttggatc aaatcctaac
    13981 cataaatctg cataagcatc ccgcataaat tccttatcgt gtttctttaa gtcaacgata
    14041 catagaaccc ctttgttctt cagaacacga ctcgcttcat ttaaaacgag tgggggattt
    14101 gaaatatgat gtaaaaccat cgaggcgact accgcatcgg ctgttcctgt cttaaatggt
    14161 aaattttcta aaggggcttc tacaatttta attttctttt gattggagta agtttttcta
    14221 gcttcctcta acattttttc tgatgaatca attccaatta cttgttttga tttttttagt
    14281 aggtagggga ataatcctcc tggaccacaa cctaaatcta aaatagtttc tgatttatca
    14341 ggaagataag ataaaatttg atttctataa aggtctggat ttaaaacttc ttcttgaatt
    14401 cgatcccagt ttttacctac tttgttgaaa taacgggtgc ttttttcttc tcgatttctt
    14461 aataaaattg caaccttttg ttgatctgtt tctctgtgtg gtaaatcttc tttataagca
    14521 agaattaagc ggttcatatc cacagcaaag gaaggattcg cagttgtgtc ttcagggagc
    14581 ttgtaataaa cccaagtgcc ttctctttgt gattgcaata gacctgcatc atgaagaatt
    14641 tttaaatgtc tggaaattct agattgtccc attcctaata cttccgtgat ttcatttaca
    14701 ttgaatgaac cgaaagaaag aatatttaaa attcgaatcc tagtttcatc agaagctgat
    14761 tttaaggcac ttaaaatcat ttttccttga ttcgagtttt ttatttttga aatggtttcc
    14821 atatctatat aaccatatat caagatatct tgatatatgc aagtagtatt tataataaat
    14881 ctgtatgttt aaataatgaa agaattaaaa ggttctggag aattctttca atttacttga
    14941 atatcatcta aattattaat tctctaatat taattttata aacaccttat ttattgtagc
    15001 cataaaaatt gctttattaa aatgtttctt taggatttat tctgtgttta gttttttcta
    15061 tccaaggcag gttttatttt tatccttcaa ttgttctgta aaaaggcgat taacgttttg
    15121 cgctttggta tttgttttaa tattaggggc ttgctccaaa acaagttcaa ctattaaact
    15181 agcagagaag ggcgtattag atttatctaa ttggagttgt gatgagaacg gttcagttcg
    15241 gttggatgga gagtgggagt tttattgggg gaaatattta gaaccgaatg actttcaaag
    15301 tgcaagtctg gaaaaagatt acataactgt cccccatcgg tggaatcaac ataagatagg
    15361 aaatgattat ttacctgcag atggatatgc gacctatcgt ttaactatca ttctccctga
    15421 atctaataaa gtaaaagcat tccgactttc cattcagaac acagcttata agctctggat
    15481 agaaaatgat ctgattgtag agactggaaa gtttggaatg acgaaagaat ctatgattcc
    15541 gaagtattta ccgagaactg ttgagtatta tacagacaaa aggcaaatta caattatact
    15601 caatgtatca aattttcata atcgttttgg cggaattagg acttcgatag aatttgggag
    15661 ttctgagaat atcaaagctt ctagagaaag aagcattgct ttagatctct ttgtatcagg
    15721 tagtttattt attataggac tttattatat tggaatttac ttacttagaa gaaaagaatc
    15781 atcagctctt ttttttgcag tttatgcttt aatgtattcc ttaaaatcat tattagaagg
    15841 agagagattt ttagttcatc tatatcctga tttcccttgg gaattggatt taaaaatttc
    15901 ttatttagta acattttctg cgcctgccat tttcggaagt tttctttata gcctatttaa
    15961 tgctgaattt cagaaaattg taattcgtat cattcaattt attagtttta ttttggtttt
    16021 aattgtttta tttacgaatg ctcgttttta ttccattctt gcgatacctc atcagattta
    16081 tcttattctc gttggcttgt atgcctctta tatttttatt aaagctataa aacacagtag
    16141 ggcgggcgca aaaattttat ttattgggtt tatcgtctac ttcatattag ccgctattaa
    16201 tgattctttg tattcgagtg ggatgataca aagcttttat atgtcaggct atgctctttt
    16261 tttatttgta ttatcacagg gtttagtgct ggcgattcga ttttccaatg catttttatc
    16321 tgttgagaga ttaactactg aattatcaaa aacaaacaaa gcttatagtc gctttatacc
    16381 ggtagagttt ttgaaatttc ttaaaaagga caaaattcag gatgttcaac tgggtgatca
    16441 gatccaagaa cagatgacta ttttatttgc ggatattcgc tccttcacaa ctctttctga
    16501 aagtatgaca ccattcgaaa attttaattt tataaattca tatttaaagc ggataaatcc
    16561 aattatccgt aaaaataatg gttttataga taagttcatt ggcgatgcag tgatggcaat
    16621 atttccacac tcagctgaag atggagtaaa agctgctatt gaaattcaaa acgagatgaa
    16681 ggtttataat gagcatagaa gaaataatgg ctatcgtccg gttgaaattg ggattggaat
    16741 caatacaggt ctattaatga tgggaacggt aggcgataag aatcgaatgg atactactgt
    16801 aattggagat acagttaatt tatcttctag acttgaatca ttaacaaagg aatatcgagt
    16861 tcctattata gtatcagagc attctgttgc aaaaattcct tcattagatc agtattattt
    16921 acgtgagatt gatgttgttc aggtgaaggg caaatccaaa cctgtatcta tttatgaatg
    16981 ctttgcaaca gcagattcga aagagatcga aaataaaaca aactctgcat cactattaat
    17041 gaatggttta attaaatttc atttaggtga atatgaagag gcattagaaa attttcagaa
    17101 agctcaattg atatctgtcg atgatccaat aatccaaatg catattgatc gatgcaaaaa
    17161 tctaattcaa tcaaagaggt aatatgttaa ataaggagag taacgttaag tcttatttgg
    17221 tcacaggggg atcaggtgga ataggaagag cgattgttaa agacttagca gaaaactact
    17281 tagtttataa tttagatcaa aaagctcctg atgcaaaact aaaaggagaa atttttattc
    17341 ggacagatct gacctcttct gatagtatac aatctgcact ttactcgatt aaaaaagaac
    17401 aactttgtgg aattgtttat tctgctggat atggaggacc gtttgttgat ataacaaagg
    17461 tcacagagga tttgtgggat aaagtatttt ctataaatat aagaggggct tttatattgg
    17521 taaaggaatt tctatcttat tttcaacaga atcgatttgg tcgaattgtg gtaatcgcat
    17581 cttcgcaaag tattgtaggt gcaaagcact ctgttgccta ttcatcttct aaacatgcgt
    17641 taataggatt tgttaagtct attgcagatg agtggggcga atacggaata accgcaaatg
    17701 caattagccc aggctatgtt gatacagcaa tgggtgttca agaaaatgaa gtctctgatc
    17761 atttgaagaa aattttagat aaaactcctt ctaagaaaat tgcaaaaccc gaagaaattt
    17821 cgaggattgt taaattttta atttcggaag agtcgggata tataaatgga gcaaatattg
    17881 ttgtggatgg tggaattact gcaatttaaa accaattttt taatttaaag tataaagcca
    17941 tcattgttcc agttaaaaac ataattatta atgtcatata aaaaccgatt tccatttcta
    18001 aaaatggcat atatttaaag ttcattccat ataatccagc tatgagtgac attggaagca
    18061 tgatagcagt catgattgtg agaattttca ttatattatt tgacttgcga gtggaaatag
    18121 ttagatgaac ttctaatgca gatgagatca tttcttttac tgtttctgca atctctaaaa
    18181 ttcgaataga atggtcgcga acatctctaa aaaaagcgtc agactcaaca gatataaaat
    18241 cagtattacg agtctctaac tcttttaaaa tttcaatgtg tagattgatt acttttttta
    18301 tttgctgtag attcccgcgc atcataaaga tggagttgat atcaagttct gtattcgcag
    18361 caaaaacttg agattctaat tcttctgctt gatcttcaat tcgatataca attggaatta
    18421 tatgatctgt ttccacatca atgattttat ggataatgaa ttctattcct ttttctaaga
    18481 gaattttatt tttattccaa tccacaatta aatcgcgtat tgtgtttcta tgatccaaaa
    18541 caattgttat taaactattt tttgttacga caaaattgaa attttttgcc gttatgcgat
    18601 tgttctctaa atgcaatcct ctaaaaacta aaaaagtata attaggaaat tcttccaatt
    18661 ttacacggct atggaaattc attatatctt caatcgtaag ggaatgtatt tcaaattttt
    18721 gaagtagtct tgttagttct tcttcatttt ttggagtcag atgtatccac gttttagttt
    18781 taggaattgc aagtggaata ttagtgtctt taatttcttt tttttctttg tttgttaata
    18841 tccagttact taaaaacatt gagaaatcca ataattatct gttacctaga aatgcttttc
    18901 gtagtttgag tattttttca gaaggcacat tgtcaaaaac taaccgatag gttctagatg
    18961 tttcacactt taattttctt tcgatgattt tattttttcg agaaaggagt agggtaatct
    19021 catcaccagt ttctaaactc gctttagttg attcaatcat ttctctgctc gctctctgtc
    19081 catttatagc gatcaattcg tcattgaggt taatatctgt tttccctgct gttttatgtt
    19141 gatagatttt tgaaataaga attttcccat cacgttcttt tgtatcaaag ccaaaactaa
    19201 ttttagaatt aacttcttct cttttaattc caacttcttc taaaaaatcg aaaactggaa
    19261 ttcgaattgt atctgataaa tacttttcga atttatcttg taaattaaat cctaaaactt
    19321 cttcagatgt ctgaaagaat tcttctttgg aaaagcctct atttttttta atataaaatt
    19381 tatggtataa agcttttagt atatcaataa aagaatattt acaatctgtg ttttttaaaa
    19441 tataaatatt catacacaag actaagatcg aacctttaat atagtaggag attcctgtgt
    19501 tgtgtgagtt tggctgttgt ttgtaaaatt ttgtccaagc agtaaaggag gaatcttcta
    19561 gactcatatt ttcatcgcca atagaatcct ctaatctaga aatatctgaa agtaattcag
    19621 ctaggtagtc ttcattagaa taaaatccgc attggtgtaa aatgtagtta tcataaaaac
    19681 tggttatccc ttctgcaatc caaagctctc ttgttaaatt tggttctaaa tagttataag
    19741 gtaatagctc tattggtcta attcgtttaa cgttccagag atggaaatac tcatgagcga
    19801 gaagtcccag tagccgattg tattcatttc tttcatgcaa ctttgatggg tcaaatgcat
    19861 ttacacttga tgaggcatgt tccaatccgc cataaacatc atctgataaa tttaatataa
    19921 ataagtagta tttattggga ttaataccaa ataattttgt ttcgtagtcc gtgattttct
    19981 ttaaatcaga gatgagattt gctttaatct gtttggaaat atttccttca attattaact
    20041 catgatgaca atctcctgtt tcaaaagaaa ttgattcatg attagagata tgtattggag
    20101 aatcataaag ttggtcaaag ttagacgcaa tataatgatt ttcctttttt tctaaactcg
    20161 aataaatgta tttaaaatat gaattcgtat caattgaaat ttcgatctta ttatttagaa
    20221 agccttcagg atataaaaat agagaagggg gatttataaa tccatactct gaatctaaga
    20281 aatttgttct cactgaattc tcaaaagcat agactctgta tttaattgta aatggctttg
    20341 agtcggatat tatttcccaa cttgataatc caatctgttt ccattttaac ggattaccct
    20401 tattagccac cactgaaaag ttatgcaaat gacttgaata gtctcggatt aaataggatc
    20461 caggagacca agttggcata tgaaaaataa ctttgtcatt tttgcttttg aatgaaattt
    20521 ctacatgaaa gtaatttaaa tgaggctcag taataattac tttgtaatgt aattgagttt
    20581 tgtttgtttt taatggtaat tttttttttg gagccaaacc agattccttt tgattaaagc
    20641 atttttaagg gaagtttagc ttgtgaatag gtgatatttc tggatttatc atccagaaat
    20701 atcgttagcc actagatagc ttttacttgt gctgctgctg gtcctttttt accattgatg
    20761 atctcaaatt ctactctctc accatctttt aattgtttaa atccatcctt cagaatttct
    20821 gaataatgga caaaaatatc ttccccgcca tcaatagaaa tgaaaccata gcctttcact
    20881 ttattaaacc atttgactgt tccttgtgcc actgctttat ctccttatgg aagtttattt
    20941 atccattagc ctaagtgaaa taaggtgaga gaggtagagt aaaaacttct tgcgggagaa
    21001 ttgttactaa aatacttatc tgcataactt aagattactt attgctaaag aattataaaa
    21061 tcctaaaact agtataatac tcagaatatt ttttgtaaat aaaaaaatta ttaaatatat
    21121 actagaatga aaaagtagta catgattaat tagaatttta atttatttgg ataggaacta
    21181 tatcctaatt ggttgccatc aggatcgttg aagtagaatg tataatccgt tcttttgata
    21241 atcgttagac caatatcggt gaagttaaaa tgcggttcaa attggaagat aagtgcttca
    21301 ggagctttag aatatgggaa tttttctagc atgagcaaac agccatttgc cgtatcaaac
    21361 caaactgacc tcaaacttcc gtccactgta aacttatcct ctttccaatt taagcctggc
    21421 aaggtcttat agaattcagc catttttaaa acgtttgtgg tggctatcgc tagatgatga
    21481 atcattttag tttatgggat aattgctgtt atgtttgggg tatcgagaaa catgtattat
    21541 gtttctcgat agtatttggc gattttttgg atcgtttata ggtggaaaga ttattgaact
    21601 ctagtccaag tttgcttctt gccgagccat ttactaattt tcaagtgacc gtttaatttt
    21661 aggctgtttg cgccgtccat ttttaattct ccaacataga gtttgccgga ttctggatca
    21721 tagatttcgc catttgtcca ctcatcatcg cctgcatcat atttaaaacc ttttaaaaaa
    21781 acgaggccaa cgataggttg agttctttta gatgcatctg gattttctgt gtcaactttt
    21841 gcatcgcctg cttttgcttt tccgtctttt actgcagcgt ctgattcagc atatgttgga
    21901 aattttaacc aaacgatttt tccgcaaaca gcatccccac acttagatag ttccacatgg
    21961 gattttccac cttctgttaa ccattttcct aaaatcttat catttgcgga aagacttcct
    22021 gcaaagaata ttgctacgaa tgcagcaact gctaaaaact tttttttcat tcgaatctcc
    22081 ttgaattttg gtctttttgt agacttggat gatcatattg attttagaaa aatggcaagc
    22141 taaaatttta aaaaactttt atttcataaa aaaactaaga aatcttactt gtagagcgtt
    22201 aattcttttt gcagtgcaaa atataaatac attataggat gcctgttttt tttagagaat
    22261 ttctaagaat ctcaagttca atgtacaaat gaaaaacccc aatattgaat ttggaatgtt
    22321 gaaaagagtc catttgaatt gtcagaagat cactatcgat tttgtgcaat agagagttcg
    22381 ccaattcttt tcttttcata ctctctaagt cctcactatc tacacagagt tttaattggc
    22441 aaagttcgga ttctatttcg attactttct catctctatt cattgtagtc ctccttggta
    22501 tcaggtatat ttactagtta aattttatta ttaattagat gtatttactt ggtcaagtcc
    22561 tatttattga aaaatgcaat gcttttgaaa aattttacta cttctagtgt attagattga
    22621 attctggttt tgtttagttt tttagtgggt tgaatgtttt tagaattgat tcgaaaagaa
    22681 gactttattt gttaaaaaca atttgcaaaa ttttgcaatg aattagatac cgtggatagt
    22741 cgtgagccaa aacaatttaa aaattcagat tctttggagg gtgggtctct ttatcatctc
    22801 tatccttttt ttagattggt ttttcttttc ttatttattt ttaaaaattc caaatgaact
    22861 cgaatgggat acatctccat ggtataattt tttacagaaa aaagaaacga tctcatttcc
    22921 agaaaacaaa aaaaaggtct tagtaacagg aagttccgtt gcgctatatt cctatttgcc
    22981 tcaggacgta aatttagatt tagtaaatgc cagcattcag tctgactttt atagccatgt
    23041 tgcaatgtct cccacggatt tttattatta tgcggattcc ataattgcga aaaagcctga
    23101 tttagtttta tatcttataa acccaggtga tctacagatg gatcatttcc caaaggctaa
    23161 agatgggcag ggatttctgg attactctga agaggggagg cttcatgctt acgctgaaag
    23221 gcatccggtt aagttttatt atccgctaaa ctttctaatg gaaaatcttt cttccttaag
    23281 aaaaatagaa attttacatc tactaaccaa atcaatttta tatgtcaatc gatatcgtag
    23341 ttttgctttt gatccgatag atgcttacct agaaagacat acccgaagga agactagcta
    23401 tcataattac acaggtgtta ccttgaatcc tatacaatgg cggaaaggct ggacttcgca
    23461 aaaatttgaa gtagaatgtg aaactaaaaa agataatttc ttttctgata ctatttttat
    23521 accggaaaaa gacactacag taaaggtatt tgcggctaat ggtgatttgc ttgcaaatgt
    23581 ttcctataca aaagctggtt ggcatatact ggaatttaat ttttcaagcg ggcagaataa
    23641 gtttcatttt gaagtttcaa aggttgttag ttccaagtta atcgacaata aactttatgg
    23701 gaaagagtat ttttatggaa tccgtctatc gcaaaatttc tgcaaaaatg agttagagca
    23761 aaatatcgct tataatcgat ccaatagtca agaagatgaa attttaaaag atatgtcaaa
    23821 tgttgattat gaaacagatt attttactag gatgtataaa gatgcggatc aatatcatga
    23881 tctagaaaag aaaattcccg ttcgtccaga agttcaaagg ctctatcatc tacataatgt
    23941 gaaaaaatat ttgaaccaaa agggagcata tcgttggtca gaattttcaa tgcttgaaag
    24001 agctgccaaa aaaatgcagg atgctaagat taaatttgta attataaata atccagaaaa
    24061 tccattggag ttgaatcttt ataaagatag tatttggtat aaagattatc taaatttttt
    24121 ccaagagatg tctcagaatt attctatcaa attttatgat cataaagaat cgaatctaag
    24181 tatccaagac tttgtagatt ctcatcattt aacctatgga ggatctaaaa agatgaccaa
    24241 aatttattca gaaattatta aggataatat acgttagcct atgaatacag ttattaactt
    24301 tcttgaaaaa tcttatatta gatattctct ttttctcatt gtatactcta tagttacaat
    24361 ttcactttgg cagaaatata attacaatcc tacttccatg attaatttcg gaatggaatt
    24421 tgctgttcaa aacaaggaaa aaatgccaga tggcgcggta gtgatacttg gaaataaaga
    24481 tgatttaggc gctggatatg atgggcagat attttatttt tactcaagaa ccattagtga
    24541 gttgaatatg aattggcctc ttggttttga tgaaagctat agagcacctc gtatcggtta
    24601 tccattgtta gttggtatca ttggattctt tggcaagatg ggagcagtct atggaatgta
    24661 tattgtaaat atttctctct tcctcctatc gtatatattg ctaagaagaa ttttagatga
    24721 agataagaaa atctattcta ttttttattt actttcacct ttttcacttg ggagctatgc
    24781 tgttcttgtg agtgattctg taatggtatc tttggttatt atcgcatatt atttttatca
    24841 ggaagaagaa agcttgtatt ttgttttagt tgcgagtctt gcaatactat gcaaagaacc
    24901 agctttattt ttattctttc ctctaggcgt aaaggcatta atagataaac gtattccaaa
    24961 agcattactt gtcttatcaa ttttagtaat tccaatttta tggcatcttt atctgaaggt
    25021 aacatttcca aattggaggg caactagatt aacagatttt attttgccac tagagggaat
    25081 cattacttat actaaatcaa ttttaaattc tgtttcgaat acaaccgatt acaaagagtt
    25141 ggcacgtctt ctttctcgtt tccctttact agtcttacta atggttggac ttataacgtt
    25201 atttacaggc aacatcaagc gaggaattga gtttagactt ggtatcgggc ttgtattctt
    25261 tatggtatct actgcaagct attatcattt ttggtcagtt tatgaaaatg tttccagaat
    25321 gtttactcta agtattccgc tttttattct tttgaagaat gatgaggaag atactaaaac
    25381 gggtttatac tttttactta ctattttaat tctgctttta tttttagtaa aggtagtatt
    25441 gatccaaaag actcaaagct atacaatttg gggaatgtaa gtcccgatgc ataaaaaaaa
    25501 gatcatagtt gttggggctg gatctggtat cggaaaagcg ttatttgaaa gagttcgaaa
    25561 aacaaatgat tctgatgtat acggcttatc ccgaagagga gaaagttttt cgaataaatt
    25621 tcaatcagga ctcaactatc gttgcgatgt gacaaacgaa ggagatattc acaatttttt
    25681 ttataagtat caggaatcaa ataaaacatt ggatgcttta tttctttgtc aaggagatgg
    25741 actctttggg gagatagaaa aaatagatag actttctcta gaaaaacatt ttcaattgaa
    25801 tttattttct caattttcaa tcttacaatt tgcttatcca ttgcttcgta tttctgatca
    25861 tccttttgtt tgttttttat cttctacagc tggtaaagtt ggtttccctg aatccactgc
    25921 gtattgcgca tctaagcatg cagtggcagg gttagccaag tctttgcgag aggaatggaa
    25981 aaaagataac ataagggtaa tgactgttta tcctggagcg ataagcacat ctatttggga
    26041 gggaagagag ggttttgatc aaaatgatat gatagcgccg gaagactttg ctgaatatct
    26101 tgctagtttt ataactttac caaagacaat aaatttagat gaaagctatg tcctgcctca
    26161 gaaagggatt ttataatgtg cactcgtttt agtttagttt ttaatcaatt attagaaatt
    26221 caggaacaat ttaatactca caacgtttta gatggaatgg attttaaaaa aatgtattta
    26281 ccaacagacc ggatactagt gatttaccga aacgaagaat taggtaaaaa tgaaatgata
    26341 aaaatgcgtt ggggtttgat tccaaatttt attaaggatt ttgatcagat taaaaagtat
    26401 tctctttaca atgcaagatc tgaaagctta gaagaaaagc aaagttttaa aagtttattg
    26461 aatcgaaatc ggtgtttaat ccctgttgat cttttctatg aatataaaac ggatggggag
    26521 gtgaaggagc aatatgcatt ttccctgaac ccagaatcta gatttgctgt tgcgggactc
    26581 tgggaccttt ggacgaatcc taagacaaaa gaaaaaattt attcttgcac gatgatcact
    26641 accattggga atgaaatcgt tgcacccata cacgagaaaa atcgaatgcc tgtaatttta
    26701 gagagagatt tatatgacaa atggctagat ccaaatatag atttttctgt attgaaggat
    26761 ttgattgtcc catataatgc aagtaaaatg aaagcagtta aaaatacgga taaagataat
    26821 ttgcctctaa atccaagaat aaaaatttcc aatcaagaga aagaagaatt aaaccaacct
    26881 tctctgttta gttctaaatg aaatattttg aacgaaaaat atttttgctt ttttggatcc
    26941 aagaaagatt tttaaacaaa ctgtttgtaa ttagggtttt ggaactatcg attttattca
    27001 gtatactcac tcaatgtgtt cctcaaattt ataaagattc tccaggcttt attggtttga
    27061 aagataggtc aaagactaag actgaatatt ttcctgagaa cgcaaagtat cttggtttta
    27121 aggaaaattt taatccagac ctcaaccaat tcagacaatt ttcaaatcta gaatcacttg
    27181 aaatttcctc tccggagctg aaagacctat cacaattggg gagtttaaaa acgattcagt
    27241 atttgaattt aagtgggacg tcagtagaga acttaatgcc catttctgaa ctctcaaatt
    27301 tacattcatt gattttaaat caaacattgg taagtgattt gtcctccatt tcgaaattaa
    27361 aaaaaataac aagactttct cttgcgaata ctaaaattaa gaatattgag ttctgtgagt
    27421 attttccaga acttaggcgt ctagatatta gaaacacact cgtagaggat ttaaaaccaa
    27481 ttatgaagtc tgagaagcta atagatttgt atattggagg aactaaaatt caaaacttga
    27541 aacctttgta tggactaaaa gatttggtct atttagaagt tgattcaact tcaatagcgg
    27601 agaaagatgt attggatctt agaaaggtat tgccttattt aaaggtagtt cagagaggct
    27661 tttagtcaaa aaagcctgtt tgttttggat ttgagatttg aatcaactca ccaataggaa
    27721 taaattccga ggaaacaaag ctacccgttg aatcaaccgt ctctaggact tgaaagggaa
    27781 tgtttttgta gagcccattg gagccttttt ttatatatct catttggagg ctccaagctt
    27841 gcgatatatt ttcttctttt gagacaacga ttgcagaaat ggaattctca cttggttttg
    27901 aatctgactt tattgtataa ttttccaaaa tgccaataga agggcaaata gaaatctcct
    27961 gcctattttt ggtgaattta atatttaggt gatcacaatc gaaaccctga atcaaaatcc
    28021 atctcagtcc cagttttttg gttgcgaaga ctgaacacat ttcgggataa aaatcaattt
    28081 cattttctga atttgtttta atggttccaa tgattctaag ttcttcccat ttccttatct
    28141 tgttataatt taattttgca agaataaacc gatcctgctg aatgaatagt tgtataaact
    28201 caaaaccatt tgtgaattct aacttatttg agaaccagat ttttgctggt atgaagtcac
    28261 tggcagataa acagatcgga aaaataagaa ttagaaaaat tgttttattc agtttcattt
    28321 gataattctt ttcaattgat ctgtctatat ttaatagtca aacaaaaact gtttcatata
    28381 aattatttga caatacagaa ataggatacc tagataaaat atgcgctcca ctctgactct
    28441 tctcttccaa acttttttct tattaatttt tataattcag tttaatggtt gttatactca
    28501 atccaaatct agggactcaa aaaatattat gagtattcta tccttgaagc ttgtaatcga
    28561 tgctcaaaat tcagggtgta atggaggctt ctgggttaga aatttacttt caagcacaca
    28621 ggcaagttat tgtgtaaatg ctaaacttgt ctcttctacc acaaacatgg atttatattt
    28681 ggacaataat ctcacaacta acttaaacta ttcaaatata actaatgcat ttgaaacaaa
    28741 tatttttcct atagaaagag atgcatatgg ctccccctct gacattaatg gggataagaa
    28801 agtaacagtt ctcattttgg atataaaaga tgggtcaagc gcaaacactg gttttattgc
    28861 aggatatgtt gatccaatta atttttatat tgatagtcct gggagtggtc ttcgatcgaa
    28921 tcaaagagaa attttattta tggatggagt cgaattagtt gcgttacgcg atagagattt
    28981 ggcaaatgga aagccagata cttttttatc aactttagct cacgagtttc agcatctaat
    29041 tcgctttcaa tattctagag gaactgatga cacttggatt gatgaaggca caagtgaggt
    29101 aacaagtgat ctgactggat atggtccgca aagcgctcgc tttagttgtt ttaagggtga
    29161 tgccaatagt tcttctgctt gtttgggtgg gattggaagc acaagtttgg ggagtccatc
    29221 tctttttaat tggagtggaa ctttaaagaa ttatgcgtat gcgtatacat ttatgaaata
    29281 tatttatgaa agttctggct caaactcaac aagtagaaat acttttttta gacaaacagt
    29341 gcaaggaaat aatggcgtta ggggaaatac tgcttataat cttatctcct tgtttatggg
    29401 atctagtaat ttcaatgcaa atgttttgaa ctcggataat aaaactacat ttaaaatgct
    29461 aatggcttca tttcttgggc aggcggtagg gtattcaaat ttgaataatg tttattttgg
    29521 aaacacaacc ccagttaata tagatagtgt aagaactaca tatccgtttt cttctacttt
    29581 aactttgctt tcaagtccca acccattctc tggaataaat acatcttctt ttaatttatc
    29641 tccttcccaa gtaagtagag ttataaagag cacgacaggc gtgacatcta actcgtcaga
    29701 tatagttatt gttagtaatg gttctagtgg cgcgaataca gagttcgtta tattcaatgg
    29761 agataacgtt ggctctagaa cggcaactac gagtgcaact gcttctacac tggagctaat
    29821 ttcttaccca gaacttgatc taaaagatac ggatgggatt atttgtccca atagtcattt
    29881 tagcaaacta aatgaaatcg atcagaaaag attagattta aagttgtatc aaaaaaaata
    29941 atcaatcttc cgaatgagtg ataaatatag atatgcttta ttagttatcg tttcctctct
    30001 aattggagtg agttttgaag tatttgcaca agagaattat acttttttaa atagagctgc
    30061 tgatcgtttt cagaaagaaa aagtagaatt aaaaaattat agagttaaac aggaagtagc
    30121 ctctaagata aaagaaacct ctggatccgt aatagaaaag agatttcaaa taggatattt
    30181 tagaagtcct gatgaattca tatttatttg tagggaatta gaaatcaatg gaatgaaaca
    30241 atttttaaac aaaccactca tagagagaac aagtaaaaca gaatgggatt ggttgtctaa
    30301 ggaaggaatt tcgttacatt catttcaaat tttatctatt gaatcgaatg cagtcaaata
    30361 tcttgtgagt ccaaagaaaa tcctgactgg cttttttcgt ggacaaatct ggatacaacc
    30421 agattccgga aggattctaa aaattattaa agaacctatc attaagaaaa aagaaatgat
    30481 gcagtatatg atagaagtaa atttcgataa ggattatcgg tttcagatgc catctagaac
    30541 gattcttcga gccgtttatc aggttgggag tcgaattact gaggttgaag tagatgctcg
    30601 atttcaggat tatgagttta atatcgattt cagtcaggag attccaaaat agttttgatt
    30661 gtattgtttg atagagctgt atttagaagt taattgaacc agcaaaagct tgcctgaatg
    30721 attagggttc agaaagtcaa tgcaaacaaa taatagttta tgcttgcaaa atatatcaaa
    30781 ttttaacaca tagatagaga atccattaag gctatgtcgc tttaaacatt gaaaatgatc
    30841 atgactcaaa attttgtaaa atttatacct ataggtgata caggaagtat ttgctacctg
    30901 tttgataatt tgaaggattt tgaaggaaag gaaatggatc agttacagtc acttatcagt
    30961 gattggaaaa acagaaaaat tcaaagaatg attcttctta aatcattaac aaagaagatt
    31021 gattatgact cagttacaaa caggttgtca actaacggtt tttcatccat cctatactct
    31081 cctagtcaag atttaaaaac acaagttcaa atttttgaga ctttattaga taaagattta
    31141 aaagggaata ttggtgtttt atttttggga agttctcctt ctctaattga gttaatcgta
    31201 aagatttcca tttatgcaga caaaaatact tctgcttcag aagtttatca atacctaact
    31261 gatgaagaac ctgctgaatt gactttagag gaattaaaga agtttcagga ttttttagaa
    31321 actgaccctt atattttaac aaagccagat agagccgaat ttaaaagatt gacacctcca
    31381 atttttgaaa agattgagac caagtctgcc ttatccgcag aggaagcaaa tgcaactact
    31441 gcttttactt taaacttaag ctctttaaac actaacttgt cggatgacgc acctctttta
    31501 agcgagactt caagaattca attcaattta gagtctttag ttgagactga ctttagaaag
    31561 gaagaatctc ctgaagagca ttatgagaat ttgaaaacaa caacgatgcc tcaagagatt
    31621 gatgatagct tatctttgga tgtagaccta gctgaggaga tcgaatatat tagttcattg
    31681 gaaagtctcc cttctaaccc tactctgcaa aaaatatcaa t
//
LOCUS       DAOQOS010000061        31622 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_631_length_31622_cov_5.951025, whole genome shotgun sequence.
ACCESSION   DAOQOS010000061 DAOQOS010000000
VERSION     DAOQOS010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 31622)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31622)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31622
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_631_length_31622_cov_5.951025"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            386..1048
                     /locus_tag="PLF66_14170"
     CDS             386..1048
                     /locus_tag="PLF66_14170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65240.1"
                     /translation="MPSCHACHSDGGGSPLNNFGKAFKDGGSNLAAFGKIAAQDSDGD
                     GTANEAEAKAKSNPGSKNSLPGKAGNWLETSSLIPKEVQAAFSNIQTWQLRDAVLTSN
                     DIEAAKKMGGNLSASDENTIYIPLENQYPVGTAIIFPVTFQAKTFYLLLTTDKQLKIK
                     QVQVLDAREVPAAKNSKVYSTFTGQQAQSLPSKNGESLDDVISNTVKNAGILLYVRLR
                     GA"
     gene            1052..1801
                     /locus_tag="PLF66_14175"
     CDS             1052..1801
                     /locus_tag="PLF66_14175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65241.1"
                     /translation="MIRKFLLVFLTLLFLLTFTLIAQQTEEFKFGDTPVSAEETIKVD
                     APKPAWMTIGGPIALLVFFFGLIFTVFKMLPYRETPLRFDLHDLPIAAQRGIGMAVIM
                     FGISFSFGAFEARYQIGLHGDAQTYFHEMGLGKLIAITHAHLIGFTTSFFIIGIPFSL
                     HFNRLKIYQLIFPLGLAASLTDIISWWGLKFVSPYFEYITWWCGFVFTVCYIWMLIGL
                     VRIIFFPNIHWFPDYINDEKLRKQKEENSKN"
     gene            1842..2606
                     /locus_tag="PLF66_14180"
     CDS             1842..2606
                     /locus_tag="PLF66_14180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HMZ65242.1"
                     /translation="MTAEQTKDLKLAVLIDADNVPYSNVKGMLEEVAKYGTPTFKRIY
                     GDWTKPTLSGWKNVLLENAITPVQQYSYTTGKNSSDSALIIDAMDILYTGGVDGFCIV
                     SSDSDFTRLATRLREAGMKVIGIGEKKTPAPFIASCNKFIYIEILHKENKYAAKVKSA
                     NKGNVKKEIEVVAKIDDDLIQMISESINDIAEDEDGWVFLGVLGSLILKKQPDFDPRN
                     YGHKKLLDIIKTIPSIEIDNRPTGKNNISHFYVRTK"
     gene            complement(3035..3208)
                     /locus_tag="PLF66_14185"
     CDS             complement(3035..3208)
                     /locus_tag="PLF66_14185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65243.1"
                     /translation="MDSAIIEYRLNEIIFLNTSDSALEIKDESVFSIEEGILEMSNVK
                     LYDTAIQLLFVAS"
     gene            complement(3190..3423)
                     /locus_tag="PLF66_14190"
     CDS             complement(3190..3423)
                     /locus_tag="PLF66_14190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65244.1"
                     /translation="MQKEDGRVYCTRNGNFRLDKNLELVSETNCKFFDRIRLLTGTSS
                     LEVRKNIDIFISVDAGLDTNIINESYTLWIQQL"
     gene            4104..4730
                     /locus_tag="PLF66_14195"
     CDS             4104..4730
                     /locus_tag="PLF66_14195"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004788688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic pyrophosphatase"
                     /protein_id="HMZ65245.1"
                     /translation="MSELKSDYVLHPWHGISPGKKVPDEVDAFIEMIPTDTVKYEVDK
                     QSGYIRLDRPQKYSNHCPSLYGFIPQTYCGEKIAKFCMDKTGRKDIEGDGDPLDICVL
                     TSSAITHGNILLSVVPIGGMRMIDKNEADDKIIAILKGDTIFGHMQDIHHCPADLINK
                     LKHYFLTYKVMPDQSDPPSVEITGVYGAQEAKQIIQTSLQDYKNKFSV"
     gene            4663..6189
                     /locus_tag="PLF66_14200"
     CDS             4663..6189
                     /locus_tag="PLF66_14200"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HMZ65246.1"
                     /translation="MHKKQNKLFKHLYRTIKINFLYSFTSILILFLYSLVSIFPQAAK
                     SSLPAKQAPIAEKKISKEEPKKNIFQNEDIFSQSDSYSYELEKKIQTWKLEEIETYAV
                     SNNPLYLAEKQNIGMARGDLITSALYRNPVLSYQQQFIPLNVTESFTSHGPSVGTNGV
                     AYQNYRQNLPGGVGGPQELAPSVSWEYDFGIIRNQKMKVATQQFQGQIAQFADFDRMF
                     RLRLRQNYWLHLYLTELINFQKEFYENYQDLLDLNRFRAEKGDIALLEYDRLELERIR
                     IERDYKDADILRAQIAKELRLLIGVAPSNQILNFRGKLQFNTTQELGINLSDFNIEDR
                     PDLQALKSKAIQNKLTIDLRKKEGYTSYLNFGGEVRLKGNEQYAGVYASIPLKVFDRN
                     QGEILKAEENYKKSQLEVEYKRKQIYSEIRAALREVIAREELLLNYREIKLLDKNKDV
                     QEKYRLAYIRGASNLVTFLEAEKNYFTVLKGYFEQLYLYYNSIEVFRAAIGKLGNINE
                     "
     gene            6182..7381
                     /locus_tag="PLF66_14205"
     CDS             6182..7381
                     /locus_tag="PLF66_14205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004458404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HMZ65247.1"
                     /translation="MNKLFFEFAKNLNKYWYVVIVLLGVGGYLIFQKFKSKPKKAETA
                     QTQTQKEKRIYISKDILKNHSLEYVHLRERSRDEEITLPGKVSYDLENLANVGSRVTG
                     RIVAVYVKEGDHVRQGSKLASISSVELSNAQSTYLKARVKLEALKVQAQRANELMDKK
                     IISSREFEIANMEYKTVKTEMETSYNTLEIFGLTRAEIESLESSNNRQQYLVIRSPIN
                     GTVTDRKAVNGQAVNIDENLFVVADLRKLWVLLDVYEKDLNSVKIGAEATIKTLGDKQ
                     EVVKAYVAHVSEVIDQIKHTAEIRLEVDNAESKLKPGQTISATVKDLSSAAKARKIQV
                     VPSEAVHKIEGKTIVFAANDDGSFEVLDVTTGDTIDDDIEIKSGIPQNKNIVSTGSFV
                     LKSEYLK"
     gene            7378..10470
                     /locus_tag="PLF66_14210"
                     /pseudo
     CDS             7378..10470
                     /locus_tag="PLF66_14210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565838.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
     gene            10621..11760
                     /locus_tag="PLF66_14215"
     CDS             10621..11760
                     /locus_tag="PLF66_14215"
                     /inference="COORDINATES: protein motif:HMM:TIGR00056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMZ65248.1"
                     /translation="MEPITKTNLASFKTFRENESLILQIDGNLNISSTPEIWRETNKL
                     LEETSFNKLIIEANGIQNCEGVGISLLFNYKRIANHKKKEFEIRGLNKKFESLLNLFT
                     HEKEAKQLVNFYDNWGTTERIGFHTVLIWEEVKDIIIFIGECSVLLFRSLKNPKKIKW
                     FDVFKISETSGVNALSIIILIGFLLGLIMSFQSAIPMQRFGAEIFVANLVSMSLFREL
                     GPLMTAVIIAGRTASSFAAEIGTMKVSEEIDAIKTMGLSPLEFLVIPRLIAAIIISPM
                     LTTFFNLAGLIGSAVVMMSFQYPFITFVNQVLSAVKNLDMLGGLFKATVFGSVIAAIG
                     CYHGLNTSTGASSVGEATTRSVVNGIISIAILDGIFSVLFYYLGI"
     gene            11808..12575
                     /locus_tag="PLF66_14220"
     CDS             11808..12575
                     /locus_tag="PLF66_14220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004786946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HMZ65249.1"
                     /translation="MNTESNSPIIQVKDFTAVMGEDVIIDNISFDVWSGEIFVILGGS
                     GCGKSTLLKHMIGLIEPATGDIQIEGQSIVNATEEEKKKIQNRIGVMYQQGALFGSMN
                     LIENVSLPLNEFTNFSPEAIHLIAQMKLQMVGLDKYSKHMPSEISGGMKKRAAIARAM
                     ALDPKIIFLDEPSAGLDPITSAELDHLIKKLSRDFNITFVVVTHELESIYSIADRVIM
                     LDKRVKKIVAMGEPAYLRDNSENPWVRQFFKREINKV"
     gene            12592..13536
                     /locus_tag="PLF66_14225"
     CDS             12592..13536
                     /locus_tag="PLF66_14225"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HMZ65250.1"
                     /translation="MRQKANYFRIGMFVIASLAIVSLFIIILGAGNLFQKKLILETYF
                     DESIQGLDIGSIVKFRGVKVGTVKEITFVQDKYRLDPDNPNFSQGRYVLVKMAVKNFF
                     NVNSKAEIDPIVKHMIENGLRVKLTSQGLTGTSYLEIDYLNKDSNEPLAISWEPTDVY
                     VPSTKSTITKIGASMDEFLKKLDAADIENMAKNLNTLVVTLDTSLKEANIGEMSNNIN
                     GLLSELRQSNKELKKIVENPKMQDLPSKLNDSLTLMSQSMNKLNNMLSNNNKDISVAV
                     ENFRIVTEDLREVSNNAKKYPSMLLFGEAPKSSEIGKK"
     gene            13533..14132
                     /locus_tag="PLF66_14230"
     CDS             13533..14132
                     /locus_tag="PLF66_14230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65251.1"
                     /translation="MKLILVILSIFLMFIGCLSISKEAPKKRFFTIEADLTKIKKAPS
                     KFNKIKVNKLKINSSYEGKEFIYRKDLDFESDYYNQFLTSPSTNITEQVISWINESKL
                     SEVVVNRISSSDSNLSLEGNINLLYADFSANKVTSIIEIEFYFVDNAKDQIIFKKLYN
                     KKISISDKDPVSILRGWNQGLSEILSSLETDLRAITLEK"
     gene            complement(14121..15758)
                     /locus_tag="PLF66_14235"
     CDS             complement(14121..15758)
                     /locus_tag="PLF66_14235"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HMZ65252.1"
                     /translation="MSRKIVIIGGVAGGATAAARARRSDESAEITILERGNYISFANC
                     GLPYYISGEIKKRSNLILQTPESFFTRYNILVKLKTEAKKIDRNRKVVIVKTESGEEE
                     IKYDKLILSQGANPILPPIPNLSPKNSFSLRTIDDMDSIHNYIDSESPKTAVVVGGGF
                     IGLEMTEALVERGLSVTLVEKYIHVMPLADSEFGEMIERSLKEKNVNVIHSDSAVDYD
                     PNEKKLILESKTKIPADMILFSVGVRPELEIAKEAGLEIGKTNGVIVNEYMQSSDPDI
                     YVVGDMAEITNRITGEKTRIPLAGPANRQGRIAGANACGENKKYTGAIGSSVVKIFHK
                     TFAMTGLTEKQSRNILASSVTVHPNHHAGYYPGSKQLTLKLTFHMETGKILGAQAFGE
                     EGVEKRVDVIATAIAGGLTIDDLEELDLCYAPPFSSANDPVNMAAFVASNQIEGFSPI
                     ITAADFMNLYKANPNLYIIDVRNPIEYQNGNVKGAINIPLHELRSRLNEIPKEQTIYV
                     HCQVGFRGHLATRILLQSGIKKVFNVTGGYKSIELFL"
     gene            15933..16988
                     /locus_tag="PLF66_14240"
     CDS             15933..16988
                     /locus_tag="PLF66_14240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65253.1"
                     /translation="MKYFILALLTFILLDCDKNRKEAPSSDRKEFFQIKDYDDLPVKR
                     GLYADHYPTSNERRIDFFMKPIESIGGCYIGVGTDQNLSFIAKAKSEFAWLMDFDPVI
                     VRVNKIHILFLQIAPTYSDFKNLWLRKNKKESYLLVVKHFEKEPDFEIIKQAWETAHQ
                     NYSGVTERLTEIEYMNKTFGLITFSNSPEEYSYIRNMAIEGRIQALNGDLMGTKTMAS
                     IASAAKQMNCPVRILYMSNAEEYFRYPENFRKNILGLPTDSKSLVIRTMTSGTKATFG
                     YPDGEKYPDTYPFHYNIQPIDNLKIWMGFKAYISSVAMLQKRTTLEKGFSILNATPLE
                     SGFVETGDITKKPPGAW"
     gene            complement(16989..18677)
                     /locus_tag="PLF66_14245"
     CDS             complement(16989..18677)
                     /locus_tag="PLF66_14245"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HMZ65254.1"
                     /translation="MKLSFGSNPDSNDFFSNKQLSEKVYFLLLGLVLVFIAFIRIRLI
                     DFPLERDEGEYAYMGQLMLDGIPPYKFAYNMKLPGTYGMYALFMGLFGETTRGIHTGF
                     IILNITTILILFFFVRKLFNSSTALISSAAYGVYALNPYILGFAAHATHFLLLFAVSA
                     CYFLLSAKEQKGSLPYFFIGFLFGMSFLMKQQAIFLILFSGIYFLFLNYLSWKEGNFE
                     LFSFFQKGLMLVIGTILPFLITVFILYIWGVFPSFWYWTFQYASKYAGMSTLSEGLIN
                     LKSVFKLLTYGYQILWILTAIGFIIYLILSHPIYKKVYLYLLVFFSFLTTTPGLYFRE
                     HYFIGFIPFAFIFFGISMEFIRIQVFNKIERMNQKHTKWTLYSSFIIICILFLVIFKF
                     KAHKDYYFKETPLRLSRQIYGVNPFQEAIQISKYIKENTKEEDQIIVLGSEPEIYFYS
                     NRKSATGYIYMYPLMEIHPYSKEMQIDLVKDLETKEHSHLIYVNQKLNYTWGILQKSE
                     RYLLDWIDKNKNEKYELVGIAEIISLDETIYKWGDEAKSYKPQSDYYIEVLRKK"
     gene            complement(18721..19539)
                     /locus_tag="PLF66_14250"
                     /pseudo
     CDS             complement(18721..19539)
                     /locus_tag="PLF66_14250"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
     gene            complement(20077..21333)
                     /locus_tag="PLF66_14255"
     CDS             complement(20077..21333)
                     /locus_tag="PLF66_14255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65255.1"
                     /translation="MKLSTEGIEFNDLKSFSNFISKLDLLGTVQLREALTDKKGNILI
                     KELVLVRESSIKKLESIADQYTPSFKIKTTPELTAKIRKALSAAIMPIVNEADKTFIS
                     TLYTGSTSGHQNIENLINNAFYTDLIVLAMYQIMEDDPNFFKYIVGMGLICLGTVIQK
                     NYTIKLIHRNAFLTGICADLSLYGTNYWKNSNLDDMLLSELTKLNVKIAENLGMTEEI
                     IYAIENHVVRGLYVVDMPEPIDMEVLKQNPFLQSSESQKENNQNPSDNEYYEDTIRIL
                     KEALKITRFISETNKKLADSDDIIQKLIIMLAYNVERKYFDPELADPMIDCFRQYEKI
                     VKRMRKIAKLENLCLHPPSAWAYPKPKAVQILCKNNHIGCLHYISGWNINVIVAQEAV
                     GYIGTNLKPGSYPKCRLEQQLDDKDE"
     gene            21468..22547
                     /locus_tag="PLF66_14260"
     CDS             21468..22547
                     /locus_tag="PLF66_14260"
                     /inference="COORDINATES: protein motif:HMM:NF024842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HMZ65256.1"
                     /translation="MKIGIIGSGITGAVLARLLPDAVVFERASHSGGRTTTRFLGNSP
                     FDIGATIFKGSIDYIEKGKLNKFDFLKFLSEHLPELEVVPHKTHPGSYHLTTAMQDLT
                     RHLLSNTKVEYNHLAESISKKPSEKEWTLHFSNGKTETFDLIILTAPVPQVISILKNS
                     GIMQAWDELIRFRGEYRSCLVLTGIWKNLPTDIIQKIKSLKQQTYLFKDEDAEYFSLE
                     SEKYRISESDNSLVVTIQFSSAFSSKNLERWCDDEKKPLYYILNSNQYFFNRVFHALD
                     LPELISKKPDHMDTHKWRYSQADFALFKDTEIQLDHPKLLEFFSLSRKHNIWMTGDWI
                     WGSRIVRCALGAMITAKEILKEGVN"
     gene            23055..23390
                     /locus_tag="PLF66_14265"
     CDS             23055..23390
                     /locus_tag="PLF66_14265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65257.1"
                     /translation="MRTILTKSFISLVLVFCLGNSFLYELLHKHKSVNLHFHEEGVLT
                     NDHTQKVDDDCALCGSFFQSRFFTNQGWILLSSITLLLLDRFFSFDVFIKSILPTTSL
                     GRSPPFLIA"
     gene            23493..24254
                     /locus_tag="PLF66_14270"
     CDS             23493..24254
                     /locus_tag="PLF66_14270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65258.1"
                     /translation="MTKFITISIIIALNILITGCEEKKKDSTPSAAALFLLSRGSTGS
                     CASGATTSISNTALGTTSASATQYTISGCDTNSLNALGFSSQNISTGATGTSVNSKIA
                     ANGDLFSAGDVNIEITFSLNSANAYLEVIGQGSGNAANIDGPAIRINPTNTQARGTGS
                     ATLANITSTTTSVGQTVTYCLDFHNESPSFHAVVWASSCTSVSTTDRNNYTGREATVP
                     STFPGKRIGFILNNATLTSFTVRTKVGTAVNLLEF"
     gene            24576..26279
                     /locus_tag="PLF66_14275"
     CDS             24576..26279
                     /locus_tag="PLF66_14275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65259.1"
                     /translation="MIIKYHYVLSIIFILFWSAFLNQKVFPDETLTMNQTHKTKVENL
                     FLYDYDQHGGKKRPPMFHIPLNVFGANQKKREEAELTSDEKYKTLVDRIIKLEAQLAQ
                     LDNKKSSNSKNTSQEDWEIQLEKDLENQRTISEKQRGQSGDNNQKNLDDSKEPLEDWE
                     IQLEREIQQQKALSKKTQNSRNTNSPTVQNPLTSNQNAQNLMMDINAAVDMVGQWDKN
                     KKKTTSNDFDIREAEFGFNAAVDQLMRGSLLVAAHNEDGKYLFEIHEAKVQFPFLMKN
                     VSATVGKMFLDQGRLNRIHRHDRPFTQAPIVHRKLMGEEANQDTGAEINILFPWSTIN
                     QELVLGATNGRVWGHSHSEGPPKNNPMFYAHLKNFYYFGNNWGSQFGFTGIRYEPDQN
                     TRTVRSQYGIDIVVKWNRSFLRSFMFMAEFWLRETEFPYERLKNNKPEMDRQLGYYAF
                     VDYQYHQQWHVGFRYDYFSVSSLRDKYGYRAPNAEIAYTPQITFKPSEFSYIRASFER
                     RYTKDFTIESSRLDFRPIDDQLRDYVENADKPKSSPTVVSYQFYIQCVFILGSHPPHV
                     Y"
     gene            26315..27250
                     /locus_tag="PLF66_14280"
     CDS             26315..27250
                     /locus_tag="PLF66_14280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015678898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HMZ65260.1"
                     /translation="MIIKLKKFFFLILFYSFTLMAEVNVVTTVTDLKYIAEQVGKDKI
                     KVESMIRGFDDPHFVMTRPDFLVKLNQADVFCQVGLDLEIGWAPLLVQQSRNIKIQKG
                     QNGFCDASVGINILGKPTVQLDRSMGDMHIFGNPHYISDPVNAIQVANNITLAFQRVD
                     PDNNDLYQQNFEEFRERLINLTKEEMKLFKPYFGSKVAVFHDEFMYLANRFRFNANLT
                     LEERPGIPPSSRYLEQVIKNMKANNIKVILISPVNNPQFAEYVASQVSGSVVVTMPTA
                     VGAMPEIKTYEDMIRTGLKKIKEALDKTKTVGGKK"
     gene            27252..27893
                     /locus_tag="PLF66_14285"
     CDS             27252..27893
                     /locus_tag="PLF66_14285"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HMZ65261.1"
                     /translation="MAYVFKTENLSVGFDMHYPILEDINITLNQGDFACLIGSNGCGK
                     STFIKTISGVLPKCGGEINIDSKTRISMVPQFKKIQYQYPLTVRDVLLLSEKFSFLKK
                     KEFTKNQMAVIQSIDIESILSLLIRECSGGQLQKVLIARSLLSDANLIFLDEPMDALD
                     SKSKRNITEILKMEIKEKNKSIFIITHNVESSWISEFNRVFVVNDRKIMETKK"
     gene            27890..28720
                     /locus_tag="PLF66_14290"
     CDS             27890..28720
                     /locus_tag="PLF66_14290"
                     /inference="COORDINATES: protein motif:HMM:NF013143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HMZ65262.1"
                     /translation="MNEILSTLKFFYPQIVIGILIGSLTACLGVILVLRRMAFFGVSL
                     SHVVSSSVAVSLFLGIKSDLLILLISTFFMIPLVMLYKNEDLSGDTILGIILVFFSSI
                     SQLLLSLGGNVKNHLMAAYFGDILTSQVKFSPEIISILLFCSLLFLILYKRILFLSFD
                     EIEFKIRKLSPRLTEFSFYFIMTVSISISVNLFGSFFSIAHLLIPVYTGLFFARSMRF
                     LYFFSIGFSVFSTLIGFLFSLKSFTYNSEIIYLPTSSTIVVTMVIISLVSFIIKKSLK
                     "
     gene            28826..29518
                     /locus_tag="PLF66_14295"
     CDS             28826..29518
                     /locus_tag="PLF66_14295"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HMZ65263.1"
                     /translation="MANYLIIGSSSGIGKEISSLLNEKKDVTVIGAGRENRQGESNFH
                     VLDVTKPETFPELDCDLHGLVYCPGTITLKSFRTLKEDDFMKDFEINLLGAFRILQKY
                     LPLLQRTNQASVVLFSTVAVGLGLPYHASISAAKGAIEGLTRTLAAEWSPKIRVNAIA
                     PSLTDTKLSEKLLDTDTKRKSAEERHPLKKIGQARDIASLACWLLTDSSQFVTGQVWK
                     MDGGLGSVKITP"
     gene            29551..29979
                     /locus_tag="PLF66_14300"
     CDS             29551..29979
                     /locus_tag="PLF66_14300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65264.1"
                     /translation="MVFLLNIFSTFFLTGLIWTIQIVHYPLFLKIGNNEFLDYHHGHS
                     FRISLIVMPMMLLELISTFLLSIVESNEYKLFHYSGLFLVLIIWLSTFFLSVPEHTIL
                     GEGWNEMSIKRLVTTNWIRTIAWSLHSLLLIIELRSKLVL"
     gene            complement(30091..30741)
                     /locus_tag="PLF66_14305"
     CDS             complement(30091..30741)
                     /locus_tag="PLF66_14305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HMZ65265.1"
                     /translation="MKHINFLFLIIFILNCNSIVQLRNQKTTASINPEDVSPGCYRQV
                     GMTPPVYKETTTTEVEKGSSKTETIEGKKETINEKVLERPAYNSCSYNGDILFCKEVP
                     AVYKNVTMTVEYPSYTKTIIIPDVQKTISKPVLVSPEKPDVRNVLCPEKATPTLIKRF
                     QVNLKSAGFDPGAEDGQLHTSTMNAIRQYEIKNGFEVAERSGEDYIMQKTADILGK"
     gene            31019..>31622
                     /locus_tag="PLF66_14310"
     CDS             31019..>31622
                     /locus_tag="PLF66_14310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015681155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HMZ65266.1"
                     /translation="MEVSVIIIIAMSILSLVTAAVFTSKVISIKVGGEDGKDTPEDSR
                     LKTISAAIAEGAMAFLIREYKVISIFIAVMTVVIFFLLDNPKTPDFNEGLFTAIAFIS
                     GAIISCISGFIGMRIATVGNVRTAQAAKESISKAFRIAFDSGAVMGFGLIGMAVLGLI
                     VLFIIFTGAFPSVEKHILMEALAGFGLGGSSVALFGRVGGG"
BASE COUNT        10484 a         4903 c         5690 g        10545 t
ORIGIN      
        1 atgccaagcc attgtttctt tgataccttt tcttggaaaa agattactcc cagaataaca
       61 atcggtatta ctgaagccgc ctttattgca cctacatacg cctgattcgg agcaagcatt
      121 ttagcctgat aagttgccag catattgacg gtgtacgtta caccaacaat catcaaaggc
      181 caaacattac tcctgacact cttattgatt gacttccaat aagttaaagt gttttgcttc
      241 cggcttttta ataggagcat tgtgactaaa attacaaaag gtaccgttag aactgaagtg
      301 taaaagttgt agcttacagg attgctcatt tctagcatgc ttacttctat gtttgaaaag
      361 atactacaaa caacaatatg gttacatgcc atcatgccat gcttgtcatt ctgatggtgg
      421 tgggagtcct cttaataatt tcggaaaggc ttttaaggat gggggaagta atctagctgc
      481 ctttggtaaa attgcagcgc aagatagtga cggagatgga actgcaaacg aggcagaagc
      541 aaaagctaaa tcaaatccag gtagcaaaaa ctctttacct ggaaaagcag ggaactggct
      601 cgaaacatct agtttgattc caaaagaagt gcaagcagca ttttctaata ttcaaacatg
      661 gcaattgaga gatgcggttt tgacttctaa tgatatagaa gcagctaaga aaatgggggg
      721 aaatctaagt gcctccgatg agaatacaat ttatattcca ttagaaaatc aatatcctgt
      781 tggaactgca attatattcc ctgttacatt tcaagctaag acattttatc ttttattaac
      841 cacagacaaa caattaaaaa ttaaacaagt gcaagtctta gatgcgcggg aagtccctgc
      901 agcaaaaaat agtaaagtct attctacttt tacaggtcaa caagcacagt ctctaccaag
      961 caagaatggc gagagtttag atgatgtgat ttcgaatact gttaaaaatg caggaatttt
     1021 attgtatgtt agattaagag gagcctgata gatgattcgt aagtttcttt tagttttcct
     1081 tactcttctg tttctactaa cgtttacatt gattgctcaa cagacagaag aatttaaatt
     1141 tggtgatact ccagtctctg cggaagaaac aattaaagta gatgctccta agcctgcttg
     1201 gatgactatt ggtggaccta tagcactttt agtattcttt tttggattga tttttactgt
     1261 ttttaaaatg ttaccatata gagaaactcc attacgtttt gacttgcatg atttaccaat
     1321 cgccgcgcaa agagggattg gtatggctgt aattatgttt ggaatttctt tttcgtttgg
     1381 agcatttgaa gctaggtatc agattggtct tcatggagat gcccaaactt attttcatga
     1441 aatggggctt gggaagttaa tcgccattac ccacgcacat ttaattggtt ttacaactag
     1501 tttttttatc atcggaattc cgttttctct tcactttaat cggttaaaaa tttatcaact
     1561 tatctttcca ttgggtcttg ccgcttctct cacggatatt atttcttggt gggggctaaa
     1621 gtttgtaagt ccttactttg aatatattac atggtggtgt ggttttgtat ttacagtatg
     1681 ttatatctgg atgttaattg gtttggttcg tattattttt ttcccgaata ttcattggtt
     1741 tcctgattat atcaatgatg aaaaattgag aaaacaaaaa gaagaaaatt ctaagaacta
     1801 gaaacaatat tattaaatga aatctatttc aggtaagtaa aatgaccgca gaacaaacga
     1861 aggatttaaa attagcagta ttaattgatg cggataacgt tccctattca aatgtaaaag
     1921 gtatgttaga agaagtcgca aagtatggga caccaacctt taaaaggatt tatggagatt
     1981 ggactaagcc aacgctatcc ggttggaaga atgttttgct tgaaaatgca attacacctg
     2041 ttcaacaata cagttatacg acaggaaaga attcttctga ttcagctctt atcatagatg
     2101 caatggatat cctctacaca ggtggtgtag acggtttttg tatcgtttca agtgatagtg
     2161 attttacaag attagctact cgtctaagag aagccggtat gaaagtcatt gggattggag
     2221 agaaaaaaac tccagctcca tttattgctt cttgtaataa gtttatttat atagaaatat
     2281 tacataaaga aaataaatat gcagctaaag tgaaatcagc caataaagga aatgtaaaaa
     2341 aagaaattga agtagtcgcg aaaattgatg acgatcttat tcagatgatt tccgaaagta
     2401 tcaatgacat cgcagaagac gaggatggct gggttttctt aggagtgtta ggaagtttaa
     2461 tcttaaaaaa gcaacccgac ttcgatccaa gaaactatgg acataaaaaa cttttagata
     2521 tcattaaaac aattccttct atcgaaattg ataaccgtcc gacaggtaaa aataatattt
     2581 ctcatttcta tgtaagaacg aaatagtttt tgcctgaaga gtaaaaatat cgaatgaata
     2641 aaaaagattc aatacttata acagtctttt cagtattgaa tcgaagatga tcaaggggca
     2701 aacactctat tatccttaat tatatcatat ttactctttg attctttgcg gatgatcctt
     2761 taggagaaaa aattactaat agtcaagatc caagaattta actgctgagt ctttaaattg
     2821 attcatactt cgttctggaa tcgcttcaat gctattcaag cttttcttgt ttaactgaat
     2881 tcaactcctg caaaaatagt tttttaagtt gattttcttt ttctctatag aataaaattt
     2941 gttgataggc tgactctagt tcgatacttg atcttattat ttctttaatc agaattattt
     3001 taatatcttt attttttatc agatgcgagt caagctagct tgcaacaaat aaaagttgga
     3061 tagctgtatc atagagtttc acattactca tttctaggat tccttcttct atactaaaaa
     3121 cactttcgtc tttaatttcc aaagcagagt cagaagtatt taagaaaatt atttcattta
     3181 gcctatactc tataattgct gaatccaaag agtatagctt tcatttatta tgtttgtatc
     3241 tagccctgca tcaacggaaa taaaaatatc aatattcttt cttacctcca aacttgaagt
     3301 tccggtaagc aaacgaatgc gatcgaaaaa tttacaattt gtctcagaca ctaattctaa
     3361 atttttatcc aatctgaaat ttccattccg agtgcaatag actcgcccat cctctttttg
     3421 cattttaaaa aaaactttcc ctaagacttc taaattgagt taggaatgag tgactcttaa
     3481 tgatccctgt gctttgttta atttcttatt taccattaaa ctgccaaaat tgcttttata
     3541 tccgaatgtt ttttcatttc ttaaatcttc caatcatagt ttatactcat ctattcgttc
     3601 gatttctttt gactgaattg tattgaaatg taatataaaa attagaagta atggtttgaa
     3661 ttttcctttc tccattttgc tcacatttat aaagatttct ttaagaaacg atgagtattt
     3721 tgtttttttc gattcagaag aaataaattt atgaaatgta aaaatgaaga tttaattttt
     3781 ccaagattat aaatgaatca atcttacact agattagaat caaaaacata tcataaaatt
     3841 tctaattgcc tttcgcttca tgataagtaa ttaaagggaa attaccgtta tcttctagat
     3901 aactaggaat aatactttgt gcatataaat taactagaat caaatgggtg acattaccta
     3961 atcggatact aagaatcgct ttacagaaaa ttatttttct tattctttct gatttttcaa
     4021 aaatttcatg agtgatttcc gtatttgata tttttttaga gaaattcaat ttacaaattc
     4081 gtccaaacat ttagtttgcg tttatgagtg aattaaaatc tgactacgtt ctacaccctt
     4141 ggcacgggat atctcccggt aagaaagttc ctgatgaagt ggatgcattt attgaaatga
     4201 ttcctactga cacagtgaaa tatgaagtgg ataaacaatc tggatatatt cgtctggatc
     4261 gtccacagaa gtattcgaac cattgtccat ctttgtatgg ttttattcca caaacctatt
     4321 gcggtgaaaa gattgcaaag ttttgtatgg ataagactgg aagaaaggat attgaaggag
     4381 atggcgatcc tttagatatt tgtgtgctaa cgtcaagcgc tattactcat ggaaatattc
     4441 ttctaagtgt agtgccaatt ggtggcatga gaatgattga taaaaatgaa gcagacgaca
     4501 aaattatcgc gattttgaaa ggagatacga tttttggtca tatgcaagac attcatcatt
     4561 gtcctgctga tttgattaat aagttaaagc attacttttt aacatataaa gttatgccag
     4621 atcagagtga tcctccttct gtcgaaatca ctggagttta tggtgcacaa gaagcaaaac
     4681 aaattattca aacatcttta caggactata aaaataaatt ttctgtatag tttcacttct
     4741 atactaattc tatttttata tagtttagta agcatttttc cgcaagcggc taagagcagt
     4801 ttacctgcaa agcaggcacc gattgcggaa aaaaaaattt ctaaggaaga accaaagaag
     4861 aatatttttc aaaatgaaga tattttttct caaagtgata gttattccta tgaactagaa
     4921 aagaaaatac aaacttggaa attagaagaa attgaaacct atgcagtttc aaataatcct
     4981 ctatatttag cagagaagca aaacattgga atggctcgag gagatttaat tacctctgct
     5041 ttgtatagaa acccagtatt gtcctatcag caacaattca ttcctttaaa tgtgacagag
     5101 agttttacaa gccatggacc tagtgtagga actaacggcg ttgcctatca gaattacaga
     5161 caaaatcttc cgggtggagt cggtggacca caggagttgg ctccaagtgt atcctgggaa
     5221 tatgattttg gtattatacg aaatcaaaaa atgaaagtgg caacacaaca attccaaggg
     5281 cagattgcac aatttgctga ctttgataga atgtttcgat tgagacttag acaaaattat
     5341 tggcttcatc tctacttaac ggaattaatt aattttcaaa aagaattcta tgagaattac
     5401 caagacttat tggatctaaa tcgatttaga gcagagaagg gagatattgc attattagaa
     5461 tatgatcgtc tagaacttga aagaattcga attgaaagag attacaaaga tgcagatatt
     5521 ttaagagctc aaattgcaaa agagttaagg ttactcattg gagttgcccc ctccaatcaa
     5581 attttgaatt ttcgaggcaa gttacaattc aatactacac aagaacttgg gattaatctt
     5641 tctgatttta acattgaaga tagaccagat ttacaagcgc tcaaatcaaa agccattcag
     5701 aataagttaa ccattgatct aagaaagaaa gaaggttata cttcctattt aaattttgga
     5761 ggagaagttc gattaaaggg aaatgagcaa tacgccggtg tgtatgcaag tatcccatta
     5821 aaagtttttg atcgaaatca aggggaaatt ttaaaagcag aagaaaatta caaaaagagt
     5881 caattagaag tcgagtataa gcgtaagcag atttactcag agatcagagc tgctttgcgt
     5941 gaagtaatcg ctagagaaga acttctatta aactaccgcg aaattaaatt attagataaa
     6001 aacaaagatg ttcaagagaa atatcgatta gcgtatattc gtggagcttc caatctagtt
     6061 acatttctag aagcggagaa aaattatttt actgttttaa agggttattt tgaacaatta
     6121 tatctttatt ataattcaat tgaggttttt agagcagcaa taggaaagtt aggtaatata
     6181 aatgaataag ttattcttcg aatttgcaaa aaatttaaat aagtattggt atgtagtaat
     6241 tgttttactt ggagtgggtg gatatttaat ttttcaaaaa ttcaaatcta aacctaagaa
     6301 agcagaaacg gcacaaactc aaacacaaaa agaaaaaaga atctatatct caaaggatat
     6361 tttaaagaac catagtttag agtatgttca tttaagagaa agaagtcgtg atgaagaaat
     6421 aacgttaccc ggaaaggttt cgtatgattt agaaaatctt gcaaatgtag gttctcgagt
     6481 caccggaaga attgttgcag tgtatgtcaa agagggagat catgtgcgac aaggtagtaa
     6541 gcttgcatct atttcatccg tagaattgag taatgcgcaa tcaacctatt taaaagcaag
     6601 agtaaagtta gaggctttga aagttcaagc tcaaagagca aatgaattaa tggacaaaaa
     6661 aatcatttca tctagagaat tcgaaattgc aaatatggaa tataaaacag ttaaaacaga
     6721 aatggaaaca agttataaca cacttgaaat ttttggtttg actagggctg agatagaaag
     6781 ccttgaatcc tctaacaata ggcaacaata tttggttatt cgaagtccta tcaatggaac
     6841 tgttactgat agaaaagcgg taaatggtca ggcagttaat attgatgaaa acttatttgt
     6901 tgttgcagat cttcgaaagc tttgggttct tttagatgtt tatgaaaaag atttgaattc
     6961 agttaagatt ggagctgaag caacaattaa aacgttaggt gacaaacaag aagtagtaaa
     7021 ggcttatgtt gctcatgtga gtgaggttat tgatcaaatt aagcatactg ccgagatacg
     7081 tttagaggta gacaacgccg agtccaagtt aaaaccagga caaacaatta gtgcaacagt
     7141 gaaagattta tcctccgctg ccaaagctcg taaaattcaa gtggttccat ccgaagcagt
     7201 tcataaaata gaaggaaaaa caatagtatt tgcagcgaat gacgatggtt cctttgaagt
     7261 gttagatgtc actacgggtg atacaattga cgatgatatt gaaatcaaat ctggtattcc
     7321 tcagaataaa aatatagtca gcactggttc ctttgtatta aagagtgagt atttaaaatg
     7381 attgaagctc taatcagttt ttctattaag aaaagatttc ctgtcatcat aataacttta
     7441 ataatttctc tcattggttt atacaatgcg attcatcttt ctgtagatgc agtgcctgat
     7501 gttactaacg ttcaagtatc ggttgttact ccttcaccag gcttgtctcc gattgaggtg
     7561 gaacaattaa taacttatcc tgttgaaata gccatgacag gtctaccaaa tgtggaagaa
     7621 attcgatcta tttccagaac gggtgtaagt agtgttacta tcgtatttaa ggatcatgta
     7681 aatatttggt ttgctcgtca gttagtaaat gaaagattaa aagacttaga agatgatatc
     7741 ccacctggtt atggtattcc tggtctatct cctgtagcaa ctggtcttgg ggatatatat
     7801 gaatttgttc taacttcaga tcgacattct ccaatggatc ttagaacgta tatggattgg
     7861 gatctttctt taaaattaaa atctttgcct ggagtgattg atgtcaatac aattggggga
     7921 gatgtaaaac agtatcagat tttaattgat ccacgtcgac taacggctca taatataaca
     7981 ttttctgcat tgctagatag attacaagaa gtgaaccgaa acgccggtgg tggatatgta
     8041 atgaagggaa atgagcaatt tgtaatcaga ggagaagggc agtttaaaag tgtagaagaa
     8101 ttagagaata ctgcaattca aacggataaa gatggaaccc ctcttttact cggaaatatt
     8161 gccacaatta aagtaggacc ttcgattcgt ttcgggctta ttacaaaaag tggggctact
     8221 aacggagaag ttgttggtgg aacagtaatg atgctcattg gagaaaattc aagagaagtg
     8281 gttcaggtga tcaaaaagcg agtagaagaa ttaaaaaacg atctacctga agggatgaaa
     8341 attgaacctt tctatgatcg atccgaattt attaaccgcg ctctcaaaac agtttttatt
     8401 aacttagcag aaggagcgat tcttgtattt attgcgctta tcgtttcttt agggactgta
     8461 aaaggaggtg cgcttgttgc tctagcaatt ccaatttcta tgttaattgc cgtaattttt
     8521 atgaggcaaa ttggagtagt tgggaattta atgagtttag gagcacttga ctttggactc
     8581 ttagtggatg gtcctattgt aatgcttgag tccgtaatgg caggattcat tgctaaaaga
     8641 tctatgtttc ctgataccct tgatgaagca gaaaaacgaa ttcgttccgt ggagttaata
     8701 caaactagct gtatacgagt tgctcgagcg gcagcctttt ctgtggcaat tatcatgctc
     8761 gtctatttgc ctcttatggt attagaagga gtggaaggaa ggatgtttcg tccaatggct
     8821 attactgttg cactagcgtt agccgctgct cttttatttt ctttaacaac tttccctgcg
     8881 gcagtttctt atttatttga aaatcctgaa ttccatcata gtcattattg ggatgtaatt
     8941 gaggaaaaat ataaaatatt tttggactat ggcttaacaa agagaaagca agtcgttata
     9001 acttcacttg ctctatttat tttttcatta gtagcaggta tgacattagg agcagaattt
     9061 ttacctcgta ttgatgaagg agagttaaat atcgacgtta agcgattgcc ctctacatcg
     9121 ataaatcact cccgcgattt gaaccgcgaa atcgaaaatg ttctaatgca atttcctgag
     9181 gtaactagcg ttgtatctaa aacaggtagg ggggaatctg cagccgaacc tgttggaaca
     9241 gacgaagggg agttaatggt taaactcaaa gacaaaaaag aatgggttac tgccaaagac
     9301 agggaatctt taatgagttt gatgaaagat aagattctaa aaacagtgcc gtctagttat
     9361 attagtatgt ctcaaccaat tgaaaataga gtgaatgcac tacttgctgg atcaaaagcg
     9421 gatatcgtta tcaaagtata tggtgataat ttagaacagc ttaagagtat tggggatacg
     9481 atagccgata tattagagaa tattgaaggc actggtgatt tgcgtgtgca acgtgtttta
     9541 ggacttcctt tactcgagat aaaagcgaac agagctaaaa tggcgcggta tggagtgacc
     9601 gcaaatgaaa tgctagacat agtagaaacc ttgcaaattg gtcgaaatgc tggtaaggtt
     9661 tttgaagaag gtctgaagcg ctttgattta gttgtcaaat tagacgtatc gtttaaagaa
     9721 ttagaatttg taaaaagtgt tccgatcatg acaacttttg ggaatactgt tcctctggga
     9781 caagttgccg atatcacaat caaagaggga cctgcatcga ttacaagaga atctctcaaa
     9841 agaagattat tcgttgaggt aaatattcgg ggaagagatt tggtgagtta tatcaatgaa
     9901 gcaaaagaaa aaactgccaa agtgattgag gacttgccag atggatatta tgtgcaatgg
     9961 ggtggtcagt ttgaaaactt tacaagagct aaaaataggc tgatgcttgt ggttccaatt
    10021 gcaatggtaa ttattttttc tatgttagtg gctgcgtttg gaagcgttcg ttatgcgatc
    10081 ggagtttttc taattgttcc tcttgctgtt tcgggtggaa tattaagttt agtttttaga
    10141 ggacttccat ttagtattcc tgctggtgtt ggttttatcg ctgttagcgg aatagcagtg
    10201 ttaaacgggg ttgtttatgc ttctgtttta cgagaacaac tagaagcagg tttgaggcta
    10261 tcggaagcag ttgtaaaggc atcgatatta tctcttcgac caattatgac aacagaaagt
    10321 attgccgcta ttggttttat tccaatggca atatcaacta acgctggtgc ggaagtgcag
    10381 cgccctctgg ctacagttgt gattggagga gtattagttg cgaccttgtt atctagatta
    10441 ttgcttccta tagctatgga gtttttactt aaaaacacga atgacgaaga accactttgg
    10501 gattctgaga ataaatccct ggaagagtct attgcaattg ccaaggaaga ttccgtttaa
    10561 attttagttg tattatttaa atagctagaa atctatttga aaagagactc taagatttac
    10621 atggaaccta taacaaaaac caatttagca tcttttaaaa catttcgtga gaatgaaagt
    10681 ttgattctcc aaatcgatgg aaacttaaat ataagttcta ctcctgagat ttggagagag
    10741 acaaataaac ttttagaaga aacaagcttt aataaattaa tcattgaggc taatggcata
    10801 caaaattgtg aaggtgttgg tatttcttta ttatttaatt ataaacggat tgcaaatcat
    10861 aagaaaaaag aatttgaaat cagaggatta aataaaaaat ttgaaagcct cctgaattta
    10921 tttacacatg aaaaggaagc caagcaactt gttaattttt atgacaactg gggaacgaca
    10981 gagcgaattg gatttcatac tgttctgatt tgggaagaag ttaaagatat aatcatcttt
    11041 attggggagt gttctgtttt attatttcga tccttaaaga atcctaagaa aatcaaatgg
    11101 tttgatgttt ttaaaatttc agaaacttct ggtgtgaatg ctttatcaat tattattttg
    11161 ataggatttt tgttaggact aatcatgtct tttcaatctg caattccaat gcagagattt
    11221 ggtgcggaaa tctttgtggc aaatttagtc tctatgtctt tgtttcgaga attgggtcct
    11281 cttatgaccg ctgtaatcat tgcaggaaga actgcttcct cttttgctgc cgaaattgga
    11341 actatgaaag tcagcgaaga gatagatgct attaaaacaa tgggtttaag tcctcttgag
    11401 tttttagtta tccctagact cattgcagcg attatcataa gtcctatgtt aacaacgttt
    11461 tttaatcttg caggtttaat tgggagtgcg gttgtaatga tgtcttttca atatccattt
    11521 attacttttg taaatcaagt tttgtctgct gtaaaaaatc tagatatgct tggtggactc
    11581 tttaaagcca ctgtttttgg gtctgtcatt gctgcgattg gttgctacca tggattgaat
    11641 actagcacag gggcaagctc tgttggagaa gcaacaacta gatcggttgt gaatggaatc
    11701 atttctatcg caattctaga tggaattttc tcggtactat tttattatct cggtatataa
    11761 atttattgac taatcgaatt acatcgatgt taaattgaag aaaatctatg aatacagaat
    11821 ccaattcccc aatcattcaa gtaaaagatt ttacagcagt aatgggtgag gatgtgatta
    11881 ttgataatat tagcttcgat gtttggtcag gagaaatttt tgtaatctta ggtggttcgg
    11941 gatgtggtaa gagcactctt ttaaaacaca tgattggtct cattgaacct gcaactggtg
    12001 atatacaaat tgaaggacaa agtattgtaa acgcaaccga agaggaaaaa aagaaaattc
    12061 aaaacagaat aggagtcatg tatcaacaag gtgcactgtt tggatcaatg aatcttatcg
    12121 aaaacgttag tcttccttta aatgagttta ctaatttttc tccggaggca attcatttaa
    12181 ttgcacaaat gaaactccaa atggtagggt tagataaata ttccaaacat atgccttcag
    12241 aaataagtgg gggaatgaag aagagagcgg ctatcgcacg tgcgatggct ctagatccga
    12301 aaataatttt tttagatgaa ccttctgctg gtctagatcc aattacatca gctgagttag
    12361 atcatctaat caaaaaatta tctagagatt ttaatattac atttgttgtt gtgactcacg
    12421 aactagagag tatttattcg attgcggata gagttatcat gttagataaa agagtaaaaa
    12481 aaattgtagc aatgggagaa ccggcttatt taagagataa tagtgagaat ccctgggtgc
    12541 gccagttttt caaaagggaa attaataagg tctgattaga ggaaaaagat tatgagacaa
    12601 aaagcaaatt attttagaat tggtatgttt gtaatagcaa gtttggcaat cgtttctcta
    12661 tttatcatca tacttggcgc tggaaatctt tttcagaaga aattgatttt agaaacctat
    12721 tttgatgaat ctattcaggg gttagatata gggtctattg taaaatttag aggtgtgaaa
    12781 gttggaactg taaaggaaat tacctttgtg caagataagt atcgattaga tccagataat
    12841 ccaaactttt cacaaggacg ttatgtattg gtgaagatgg ctgttaagaa tttttttaat
    12901 gttaactcta aagcagaaat agacccaatc gtgaagcata tgatagaaaa tggactccga
    12961 gttaaattaa catcgcaagg actcacggga acatcttatt tagaaatcga ctatttaaac
    13021 aaggattcaa atgaaccact tgcgattagt tgggaaccta ccgatgttta tgttccctct
    13081 actaaaagca ctatcacaaa aatcggggca tctatggacg aatttttaaa gaaattggat
    13141 gcagcggata ttgagaatat ggcaaagaat ctaaatacgt tagttgttac tttggataca
    13201 tctctaaagg aagcaaatat tggggagatg tctaacaaca taaatggact tttgtctgaa
    13261 ctgcgacaat caaataaaga gttaaaaaag attgtagaga atcctaagat gcaggattta
    13321 ccttcaaaat tgaatgattc acttacatta atgtctcaaa gtatgaataa attaaataat
    13381 atgttgagta ataataataa ggatatttct gttgcggttg aaaattttag aattgtaacg
    13441 gaagacttaa gagaagttag taataatgcc aaaaaatatc catccatgtt actatttggc
    13501 gaagcaccaa aatcatctga aataggtaaa aaatgaagtt aattcttgtt atcctctcta
    13561 tatttttaat gtttattggg tgtttgagta tttccaaaga agcacctaaa aaaagatttt
    13621 tcacaattga agcagacttg acaaaaatta aaaaagctcc tagcaaattt aataagataa
    13681 aagttaataa attgaaaata aattctagtt atgaaggaaa agaatttatc tacagaaaag
    13741 atttagattt tgaaagtgat tattataacc aatttttaac ttctccatct acaaacatta
    13801 cagaacaggt aatctcttgg ataaatgaaa gcaagttatc tgaagttgtt gtaaatcgga
    13861 ttagtagttc tgactctaat ttatctttag aaggaaatat aaatttgctc tatgcggatt
    13921 tttcagcaaa caaagtaacc tctattattg aaatagagtt ttattttgta gataacgcaa
    13981 aagatcagat tatatttaaa aagttataca ataagaaaat ctcaatctca gataaagatc
    14041 ctgtctctat tctacgaggt tggaatcaag gcctatcaga aatactaagt agtttagaga
    14101 cagacctaag agctattact ttagagaaat agctcgatac ttttgtatcc accagtgaca
    14161 ttgaatactt ttttgattcc agattggagc agaattcggg ttgcaagatg acctctaaaa
    14221 ccaacttgac aatgaacata aatagtttgt tcctttggta tttcatttaa tctagaacgc
    14281 aattcatgta atggaatatt gattgctcct tttacatttc cgttctggta ttcaattggg
    14341 tttctcacat cgataatgta aagattagga tttgctttat acagattcat aaaatctgca
    14401 gctgtgataa ttggactaaa tccttcaatt tgattggaag caacaaatgc agccatattt
    14461 acaggatcat ttgcggatga gaagggaggt gcatagcata aatccaactc ctctaaatca
    14521 tctatcgtga gtccacctgc aatagctgtt gcaatcacat ccactctttt ttcgacccct
    14581 tcttctccaa atgcctgtgc tcctaaaatc tttccagttt ccatatgaaa cgttagtttc
    14641 agagtaagct gttttgagcc aggataatag ccagcatgat ggtttgggtg aactgtaaca
    14701 gaagatgcta atatatttct gctttgcttt tctgtaagac ctgtcattgc aaaagtttta
    14761 tgaaagattt ttacaacaga tgatccaatc gctcccgtat attttttatt ttcgccacaa
    14821 gcgtttgctc ctgcaattct tccttgtcta tttgcaggtc ctgcaagtgg aattcgagtt
    14881 ttttctcctg tgatacgatt tgtaatctct gccatatccc ctacaacata aatatcagga
    14941 tcggacgatt gcatatactc attcacaatc actccatttg tcttgccaat ttctaagcca
    15001 gcttctttcg caatttctaa ttcaggtctt actccgactg aaaagaggat catgtctgct
    15061 ggaatttttg tcttactttc taaaattaat ttcttttcgt ttggatcata atctacagct
    15121 gaatctgaat gaatcacatt tacattcttt tcttttaaag atctttcaat catttcgcca
    15181 aattcagaat ctgctaaagg cataacgtga atatactttt ctactaacgt tacactgagc
    15241 cctctttcta caagggcttc ggtcatctct aagccaataa aaccaccacc tacaacgact
    15301 gctgtttttg gagattctga atctatataa ttgtgaatac tatccatgtc atcgattgtt
    15361 cttaaggaaa aagaattttt gggagaaaga ttaggaattg gtgggagaat tggattggca
    15421 ccttgagata agatcagctt atcatatttt atctcttcct ctcctgattc agtttttact
    15481 attacaactt ttctgttgcg atcaatcttt ttggcttcag tctttaattt tactaagata
    15541 ttatatcgag taaaaaaact ttcaggggtt tgtagaatta gattactacg ttttttgatt
    15601 tcaccagaga tataataagg aagaccacaa tttgcaaacg agatatagtt tcctctttct
    15661 aaaatagtaa tttctgcaga ttcatctgat cttcttgctc ttgctgctgc agtcgctcca
    15721 cctgcaactc caccgataat aacaattttt ctactcattt tatttctcct gaatctatac
    15781 tataccctat agggtatatg gcaaccattt ttttattaag tctagtatgt tttatacata
    15841 ctcaatactt gaataaaact tttaaaaatg taaatgactt tttaaattga gatacaattc
    15901 tgactgttaa aaaacatagg ctggtttgac tcttgaaata ttttatattg gcactactta
    15961 catttattct tcttgactgt gataaaaaca gaaaggaagc tccttcctct gaccgaaagg
    16021 aattttttca aattaaggat tatgatgatt tgcctgttaa aaggggactt tatgcagatc
    16081 attatcctac atcgaatgag cgcagaattg atttttttat gaagccaatc gaatcaatcg
    16141 gaggctgtta tattggtgtt ggaacagatc aaaatttaag ctttattgca aaagcaaaat
    16201 ctgagtttgc atggttaatg gattttgatc cagtaattgt tcgtgttaat aaaattcata
    16261 ttttattttt acaaatagct cctacttatt ctgattttaa aaatctttgg ttgagaaaaa
    16321 ataaaaaaga atcttatctt ttggttgtaa aacattttga aaaggaacct gattttgaaa
    16381 ttataaagca agcatgggaa actgcccatc aaaattattc tggtgttaca gaacgactta
    16441 ctgaaataga atatatgaat aaaacctttg gtttaattac attttcaaat agtcctgaag
    16501 aatattccta tatcagaaac atggctatag agggtcgaat acaggcatta aatggagacc
    16561 taatgggaac taaaactatg gcgtctattg cttctgccgc taagcaaatg aattgtcctg
    16621 ttcgaatttt gtatatgtca aatgcagaag aatattttag ataccctgaa aattttagaa
    16681 aaaatatttt aggtcttccg actgactcaa aaagtcttgt gattagaaca atgacgtcag
    16741 gcacaaaagc gaccttcgga tatcccgatg gagaaaaata tccagatact tatccatttc
    16801 attataatat tcaaccaatc gacaatttga aaatatggat gggatttaaa gcatatattt
    16861 catccgttgc aatgcttcaa aaaagaacta ctttagaaaa aggtttttcc atattaaatg
    16921 caacaccttt ggaatcagga tttgtagaaa caggagacat tacaaaaaag cctcctggag
    16981 cttggtgatt attttttccg taaaacttct atataataat cggattgagg cttataactc
    17041 ttagcctcat ccccccattt ataaattgtt tcgtctaagg aaataatttc tgctattcca
    17101 actagctcgt atttttcatt tttattttta tctatccagt ctagtaaata cctttctgat
    17161 ttctgtaaaa ttccccaagt gtaatttaat ttttgattca catagattag atgagaatgc
    17221 tcttttgttt ccaaatcctt tactaaatct atttgcatct ctttgctgta aggatgaatt
    17281 tccatgagtg gatacatata gatatatcct gttgcagatt ttcgattgga ataaaaataa
    17341 atttccggtt ctgatcctaa aactatgatt tgatcctctt cttttgtatt ttcttttata
    17401 tacttggaaa tttgtatagc ttcttggaaa ggatttacac catagatttg acgcgatagg
    17461 cgtaaaggtg tttctttaaa gtagtagtct ttgtgcgctt taaacttaaa tatcactaaa
    17521 aataaaatac aaataatgat aaaggaagaa tagagagtcc attttgtgtg tttttgattc
    17581 attctttcta ttttattgaa aacttgaatt cgaatgaatt ccatagagat tccaaaaaaa
    17641 ataaatgcaa acggaatgaa accaatgaag taatgttctc tgaaatacaa accgggagta
    17701 gttgttaaaa aggaaaaaaa taccaaaaga tacaaataga cttttttata tattggatgc
    17761 gagagaatta aatagatgat aaatccaatt gccgttagta tccagagaat ttggtagcca
    17821 taagttaaaa gcttaaaaac agattttagg ttgattaacc cctcggataa cgttgacatt
    17881 cctgcatatt tacttgcata ttgaaaagtc caataccaaa agcttggaaa cactccccat
    17941 atgtataaaa tgaaaactgt tattaaaaat ggaaggattg ttccaataac tagcattaat
    18001 cccttttgaa aaaaggagaa gagttcaaaa ttgccttctt tccaacttaa atagtttaga
    18061 aataggaaat aaattcctga aaataaaatc aaaaagattg cctgctgctt cattagaaaa
    18121 ctcataccaa acaaaaaacc tataaaaaaa tagggaaggc ttcctttttg ttcttttgca
    18181 ctcagcagaa aataacaagc actcacagca aaaagtaata aaaagtgcgt cgcatgggct
    18241 gcaaatccaa gtatatatgg attgagtgca taaacaccat aagctgctga agaaataaga
    18301 gctgtcgaag aattaaataa ctttctaacg aaaaagaaga gtataagaat ggttgttata
    18361 tttaaaatga taaagccagt gtgaattccg cgtgtcgttt cgccaaacaa acccataaag
    18421 agagcataca ttccatatgt gccaggtaat ttcatattat aggcaaattt atagggcggg
    18481 atgccatcta acattaattg tcccatataa gcatattcac cctcgtctct ttctaatgga
    18541 aaatctatga gccgaattcg aatgaatgcg ataaaaacta gaacaagacc taaaaggaga
    18601 aaataaactt tctcgcttaa ttgtttattt gaaaaaaaat cattagaatc gggattagag
    18661 ccaaaggata acttcataaa acgaaattaa taggaggaaa taaccattca agtattaaat
    18721 ttatgattca attccaatta ttgtaatatc atcttgaaag gagtcatcgc ctaaaaatgt
    18781 ttctaagtta ttttgaatat tttgaatgat ttcatttatt ttgtagtttt ttgtgttttg
    18841 taaaatggaa actaatcttt cctctccaaa ctcttctcta gatttattga actcctcaaa
    18901 taaaccatct gtaaatagga atagtttact accttgcgga aaatcaagcg tatcctctga
    18961 atatttttca ttgtttacta caccaatcaa ttagcctgtc ttatttaatg ataaaatatt
    19021 ttcttcttgg atcaaatatt gttccgggtg accggcagat gcatagataa gctttcgatt
    19081 tttcagatca atgtctgtca caaaacaact aaagaaaaag ctgagagaat agtatttatt
    19141 gtaaaaagta ttattaaagg atttgagtag ttgtccaggt gagagtgaca attgttttgt
    19201 tccttctaat tcacttttga tcagcatggt gattaacgct gcttcaacac catgacctgt
    19261 tgcatctgca agaaagattc taatttttcc ttctccaatc ggataaatat caaataaatc
    19321 accacctact tctacaagag ggatatactt ggattgaatt acaatggttt catatttata
    19381 attttctata ggtagcaatt ttgtctgaat cttttttgca aatttgagtt cttgttttat
    19441 atttgataaa agtttttctg aaatttcttt ttctatttga gcttttttag tcagttctga
    19501 actttttctt acaaatccat gtatgatttt atttaccata aaaagtccaa agagcatcca
    19561 aactgaagag atgagcatag aagcgatcgt tagtttagtc tctccttctg aaaaaacatt
    19621 tacatttgct aaaaagccaa tgtagactaa tattccagcg aggctatatt gagtggcgca
    19681 aagaccagcg gcaaatagcc cgaatcctct cgattgttgc atagaggtaa gagcagtgat
    19741 gatgaaataa cccgtgatta gataggaatg aatatttccg gtcatctgga tgataatagt
    19801 tgcagctgta atatctatga aactaggtat gtaaccagac caagtatgtc ttttttctat
    19861 aaagatttct gtttcaatta caaacatcca aagacaggca aaaaaaagat gaataaatcc
    19921 cgccattata agagatttat gctctatttt catatagagc attaaggcta aatcaaaaat
    19981 tgaaattaat attctcgcaa tattaattgt ccggaacttt tcaattttat ccggtgagag
    20041 atgaattgtt gacccctcta tcaaatgaaa cctcaatcat tcgtctttat cgtctagttg
    20101 ctgctccagt ctacatttcg gataacttcc tggttttaag ttggttccaa tatatccaac
    20161 agcttcttgt gccacaataa cgttaatatt ccaacccgaa atataatgaa gacatccaat
    20221 gtgattattt ttgcaaagaa tttgaactgc tttaggttta ggataagccc aagcagatgg
    20281 aggatgtaaa catagattct ccagttttgc gatttttctc atacgcttta caatcttttc
    20341 atattgccta aaacaatcta tcattgggtc agcaagctct ggatcaaaat attttctttc
    20401 tacgttatac gcaagcatta taattagctt ttgaatgata tcatcagagt ccgctagttt
    20461 tttatttgtc tcagatataa aacgagttat ctttaaagct tcttttaaaa ttctgatcgt
    20521 gtcttcataa tattcattgt ccgatggatt ttgattgttt tctttttgag attcagatga
    20581 ttgtagaaat ggattttgtt ttaatacttc catatcaatt ggttcaggca tgtccactac
    20641 atagagacct cgaacaacgt gattttctat cgcatagatt atttcttctg tcattccaag
    20701 attttctgct atcttcacat ttaattttgt taattcacta aggagcatat cgtctaggtt
    20761 ggagtttttc cagtaattag ttccatagag agatagatcc gcacaaattc cagtaaggaa
    20821 tgcatttcga tgaatcaatt taatggtgta atttttttga attacagttc ctaggcagat
    20881 taatcccatt ccaactatat atttgaaaaa gttaggatca tcttccatga tttgatacat
    20941 tgcaagcacg attaaatcag tataaaaagc attattgatt aaattttcaa tattttgatg
    21001 tccagatgtg cttcctgtat agagagttga gataaaggtt ttatctgctt catttacaat
    21061 cggcataatt gcggcggata acgcttttct aatctttgcg gttagctctg gagtagtttt
    21121 aattttaaaa gatggcgtat attgatcagc aatagattct agttttttaa tactagattc
    21181 ccttaccaaa actaattctt tgattaagat atttcctttt ttgtcggtga gagcctctct
    21241 gagttggact gtgcccaata aatccaattt tgatatgaag tttgaaaaag attttagatc
    21301 gttgaactca attccctcag tgctcagttt catatattac tcttttgaac gtattttcta
    21361 tctttataaa atataccgct tctttgtgca ttaaatattt ttaattctaa ataaggcagc
    21421 cgattcttct tgacgaatca tttcgaataa tgtattaaaa ccttatcgtg aaaattggaa
    21481 ttattggaag cggaataacg ggagcagttc ttgctcgtct tctaccggat gctgtagtat
    21541 ttgagcgagc aagtcactct ggaggtagaa ctaccacaag attcctaggt aattctccat
    21601 ttgatattgg agctactatt tttaagggca gcattgatta tatcgaaaaa ggaaaattaa
    21661 acaaatttga ttttttgaaa ttcctatcgg agcatttgcc tgaattagaa gttgttcccc
    21721 ataaaacgca tcctggttcc tatcacctaa ctaccgcaat gcaggattta acgagacatt
    21781 tactctcaaa tacgaaagtg gaatacaatc atcttgctga atcgatatcc aaaaaaccaa
    21841 gtgaaaaaga gtggactttg catttttcga atggtaaaac agaaaccttt gatttgatta
    21901 ttttaactgc acccgtccct caagtaatct caattttaaa aaattcagga atcatgcaag
    21961 cgtgggatga attgattcgt tttcggggag aatacagatc ttgcttagta ctcacaggta
    22021 tatggaaaaa cttacctact gatataatac aaaagataaa atctttgaag cagcaaacat
    22081 acttatttaa agatgaggat gctgaatatt tttctctaga gagcgaaaaa tatagaatca
    22141 gtgaaagtga taacagtctc gttgttacaa ttcaattctc aagtgctttt agttctaaaa
    22201 atttagagcg atggtgtgac gatgaaaaga aacctcttta ttatatccta aatagtaatc
    22261 agtatttttt taaccgtgta tttcatgctt tagatttacc cgaattaata agcaagaaac
    22321 ccgatcatat ggatacgcat aagtggcgtt attcgcaagc tgattttgct ttattcaagg
    22381 atactgaaat tcaactagat catcctaaat tattagaatt tttttccctt tctagaaaac
    22441 ataatatttg gatgacagga gattggatat gggggtcaag aattgttagg tgtgcgttag
    22501 gtgctatgat tacagccaaa gagattttaa aagaaggggt gaattaagtt tttgtttagg
    22561 atcatctctt tagtttaaaa agacttttgg ttgagtgctg actaaaaaaa tttcattaga
    22621 cttagcattt tttattaact caccttatgc aaaatttata aatttagaaa taaaccatga
    22681 tttttagaat attagataat gctaatgaat taacaaaatt aatagcctgc catagctcgg
    22741 ctgttggaga gcttataaag cgatggatcg acaagccatc cattttaact ttgctcaagc
    22801 ctattgtgct ttttcaatga caatagacca tacctcgttt gaatgtttgc ttcatcttgc
    22861 ataaggtgag ttattaaaag acttttatca tctttgtttt caaggtttta gtgaatcaag
    22921 cctttctttg ctagttactt ttcatagatt gtatcaggta ggaattaaaa aaatacaata
    22981 tttaaatgaa acattgttgc atttgggaaa ggtgtaaata ttctggcaat gtatttattc
    23041 gtatgagaat agttatgaga acaatcctaa caaaatcatt tatttctctc gttctagttt
    23101 tttgtttggg caattctttt ttgtatgaat tgcttcataa acataaatct gtaaatttac
    23161 attttcatga agagggagtt cttacgaatg atcatacaca aaaagtcgat gatgattgtg
    23221 ccctttgtgg ttcattcttt cagtctagat tttttacaaa tcagggttgg atccttctaa
    23281 gttctattac tctcttgtta ttagatcgat ttttttcgtt tgacgttttt atcaaatcaa
    23341 tcctacccac tacaagccta ggtcgctctc ctccctttct tattgcttaa aaaaattaac
    23401 caatcatttg gtataaattt acttaaaata ataacgaata ttcctccctc gagaaaaact
    23461 tgtgggaaat ttttaaaata ttggagaaga aaatgacaaa atttattaca atttctataa
    23521 ttatcgcttt aaacattcta attactggtt gtgaagaaaa gaagaaagat agcactccct
    23581 ccgcggctgc acttttttta ctaagtagag gctcaacagg ttcttgtgcg tcaggtgcaa
    23641 caactagtat atctaatact gcattgggta ccacatcagc atctgccaca cagtatacaa
    23701 tctcaggctg tgatacaaac agtttaaatg cacttggttt cagtagccaa aatatttcaa
    23761 ctggagcaac agggacatca gtaaatagta aaattgcggc taacggcgac ttgtttagtg
    23821 ctggtgatgt aaacattgaa attacattta gcttaaatag tgctaacgca tacttagaag
    23881 tcatcggtca aggatctggc aatgcagcta atattgatgg tcctgcaatt cgtatcaatc
    23941 caacaaatac acaagctcgt ggaactggga gtgcaactct tgcgaatata acatctacca
    24001 ctacttccgt tggacagact gtaacctatt gtttagattt tcacaatgag agtccaagct
    24061 ttcatgctgt ggtttgggca tcaagttgca catctgtttc aaccacagac agaaacaatt
    24121 atactggaag ggaagcaaca gttccttcta cgtttccggg taaaagaatt ggttttatat
    24181 taaacaatgc tacactcaca agtttcactg ttagaacaaa agtgggaaca gcggtaaatc
    24241 ttttagagtt ctaattgctc aaactgcata agaccctttc acctacatac tgctgtcagg
    24301 gtctttattt gagtgaaacc aacacagatt gtaacaaaac agaaagtata aggaaaagtc
    24361 tatttcaatg actgactctt aaattgtttt ttgttagata cagtagttca aaaattataa
    24421 atgatttttg aatcaccaat tctgttaaac atgaagagta ttgatttaaa agtttgtttt
    24481 acaaggtctt tcttgtaatc aaactttttt ctatttaata atacttcatt attttttcaa
    24541 aattaaattt ctttatttca tttttgtagg gttttatgat tattaaatat cactatgttt
    24601 tatctattat ttttattcta ttttggtctg catttttaaa tcagaaagtt tttccagatg
    24661 agactcttac aatgaaccaa actcataaaa ccaaagtgga aaatcttttt ctatatgatt
    24721 acgatcagca tggaggaaaa aaaagacctc ctatgtttca tattcctctc aatgtatttg
    24781 gagcaaatca aaaaaaaaga gaagaagcag aacttacgtc agatgaaaaa tataagacgt
    24841 tagtagatcg aattattaaa ctagaagcac aacttgccca gctagataat aaaaaatctt
    24901 ccaacagtaa aaatacaagt caagaagact gggaaattca gttggaaaag gatttggaga
    24961 atcaaagaac gataagcgaa aaacaaagag gacagtcggg cgacaataat caaaaaaatt
    25021 tagacgatag caaagaacct ctcgaagact gggaaattca attagagaga gaaattcaac
    25081 agcagaaagc tttatcaaag aaaactcaga attcaagaaa tacaaattca ccaactgtgc
    25141 aaaacccact tacatccaac caaaatgctc aaaatttgat gatggatatc aatgccgctg
    25201 tagatatggt tgggcaatgg gacaaaaata agaagaaaac tacatcgaat gattttgata
    25261 ttcgggaagc tgaattcgga ttcaatgcag ctgtggatca attaatgcga ggatccttgc
    25321 tcgttgccgc acacaatgaa gacggaaaat atcttttcga aattcacgaa gcaaaagttc
    25381 aatttccttt tttaatgaaa aatgtatctg ccactgttgg aaaaatgttt ctagaccaag
    25441 gaaggttaaa tcgtattcat cgccacgata gaccatttac acaagctcca attgttcacc
    25501 gaaaattaat gggcgaagaa gcaaatcaag atacaggggc ggaaataaat atccttttcc
    25561 cttggagcac aattaatcaa gaattagttc ttggagcgac caatggaaga gtttggggac
    25621 attcacattc agagggacca cctaaaaaca atccaatgtt ctatgctcat cttaaaaatt
    25681 tttattattt tggaaataat tggggcagcc agtttggatt tactggaata cgatatgagc
    25741 cagatcagaa tacaagaaca gttcgaagtc aatatggtat tgatattgta gtgaaatgga
    25801 atcgttcttt tttgagatca tttatgttta tggcagagtt ctggcttcga gaaacggaat
    25861 tcccatacga gagattaaag aacaataaac ctgaaatgga tagacaattg ggttactatg
    25921 catttgtaga ttatcaatat catcaacaat ggcatgttgg atttagatat gattattttt
    25981 ctgtctcaag cttgagagat aaatacggat acagagcgcc taacgcagaa attgcctata
    26041 ctccacaaat tacattcaaa ccttctgaat tctcatatat tagagcttcc tttgaaagaa
    26101 gatatacaaa agattttaca atagaatctt ctaggctcga ttttagaccg atagacgatc
    26161 agttaagaga ttatgttgag aatgcagata agccaaagag ttctccaacg gttgttagct
    26221 atcaattcta catacagtgt gtatttatac taggatctca tcctccacat gtttattaat
    26281 aaaaatttaa aaaataaaaa taaggaaact ttcaatgata ataaaactta aaaaattctt
    26341 ctttctaatt ctattctact catttactct aatggcagag gtaaatgtag taaccactgt
    26401 tactgattta aaatacattg cagagcaagt tgggaaagat aagattaaag ttgaatcgat
    26461 gatcagagga tttgatgatc ctcactttgt gatgacacgc cctgattttt tagtgaaatt
    26521 aaaccaagcc gatgtttttt gtcaggtagg acttgattta gaaattgggt gggcaccact
    26581 tctcgttcag caatctagaa atattaaaat tcaaaaaggg caaaatggat tttgtgatgc
    26641 atctgttggt attaatattt taggtaagcc gactgtgcaa ttagatcgtt ctatgggtga
    26701 tatgcatata tttggaaatc cgcattacat ttctgatcca gtcaatgcaa ttcaagttgc
    26761 aaataatata acattagctt ttcagagagt tgatccagat aataatgatt tatatcaaca
    26821 aaactttgaa gaattcagag agcgattaat caatctaaca aaagaagaaa tgaaattatt
    26881 taagccttac tttggatcta aagtggctgt attccatgat gaatttatgt atctagcgaa
    26941 tcgttttcgt tttaatgcaa atttaacgtt agaggagaga cctggaatac ctccttctag
    27001 tcgatactta gagcaagtga taaaaaatat gaaagccaat aatattaaag tgattttgat
    27061 atcgccggtg aataatcctc aatttgcaga atatgtagcg agccaagttt ctggtagtgt
    27121 tgttgtaact atgcctacgg ctgtaggagc aatgccagaa ataaaaacat atgaagatat
    27181 gattcgaact ggtttaaaga aaatcaaaga agcacttgat aaaactaaaa cagttggggg
    27241 aaaaaaataa gatggcatat gtttttaaaa cagagaatct tagtgttgga ttcgatatgc
    27301 actatcctat tttggaagat attaacataa ctttaaatca aggtgatttt gcctgtttaa
    27361 ttggctccaa tggctgcggg aagagcacat ttattaaaac gatttcaggt gttcttccga
    27421 aatgtggagg tgaaattaat attgattcga agactagaat ttctatggtt ccgcaattta
    27481 agaaaattca atatcaatac cctttaacgg taagagacgt tttacttctc tcagagaaat
    27541 tttctttttt aaaaaagaag gagtttacaa agaaccagat ggcagtcatt caaagtattg
    27601 atattgaatc cattctttca cttctcataa gagaatgtag cggggggcag ctgcaaaagg
    27661 ttttgatcgc aaggtcttta ttatcagatg cgaatctaat ttttcttgat gaaccaatgg
    27721 atgcacttga ttcaaaatcc aaacgaaata taacggaaat cctaaaaatg gagataaagg
    27781 aaaaaaataa aagtatattt attattacgc ataacgttga atcttcttgg atttctgagt
    27841 ttaacagagt atttgtagta aatgatagaa aaattatgga gaccaagaaa tgaatgaaat
    27901 tctttcaact cttaaattct tctatcctca aatagtcatt ggcattttaa ttggtagcct
    27961 aacagcatgt ttgggtgtaa ttctcgtttt gcgtagaatg gctttttttg gtgtatctct
    28021 ttcgcatgtt gtttcatcct ctgttgctgt aagtctattt ttaggtataa agagcgatct
    28081 attaattctt cttatttcta cttttttcat gattccactt gttatgcttt ataaaaatga
    28141 agatttatca ggtgacacaa ttttaggtat cattctagtt tttttctctt cgatttcaca
    28201 gcttttactc agccttgggg ggaatgttaa aaatcattta atggcggctt attttggaga
    28261 tattctaacc tcccaagtta agtttagtcc agaaataatt tcaattttac tattttgctc
    28321 tttgttattt cttattcttt ataaaagaat tttattttta tcttttgatg aaattgaatt
    28381 taaaattagg aagttatctc cgaggctaac tgaattttct ttctatttca taatgactgt
    28441 ctccatttca attagtgtaa atttatttgg atcctttttt agtattgctc atttactaat
    28501 accggtttat acagggctct tttttgctag gagtatgcga tttttatatt ttttctcgat
    28561 tggctttagt gtgttctcta ctttaattgg ttttctattt tccctaaaat cttttacata
    28621 caattcagag attatttatc ttcctacatc tagtacgatt gtagtcacca tggttattat
    28681 tagtcttgta tcctttataa ttaaaaaaag tttaaaatga tttgagagat ttttttattt
    28741 ctactagaaa attcaaaaaa tatatactat tcctcgatag ccctaaaata gatattttga
    28801 aattaagaag agtcaaagaa ttattatggc gaattattta attattggtt caagttcagg
    28861 aattggaaaa gaaattagct ctctcttaaa tgaaaaaaaa gatgtcactg taattggcgc
    28921 aggtagggaa aacagacagg gtgagtcaaa tttccatgta ttagatgtta caaaaccaga
    28981 aacatttcca gaactcgatt gtgatttaca tggacttgtt tattgcccag gcacaatcac
    29041 attaaaatcc tttcgaactt taaaagaaga tgatttcatg aaagattttg aaatcaattt
    29101 acttggtgca tttcgtattt tacaaaaata cttaccattg ctacagagaa caaaccaagc
    29161 ttccgtagtt ttatttagca ctgttgcggt tggactgggt ttaccgtatc acgcatctat
    29221 ttctgccgca aaaggagcaa tagagggatt aactagaacg ttagcagccg agtggagtcc
    29281 taaaatacgg gtgaatgcaa ttgctccctc tttaacggat acgaaattat cggaaaagct
    29341 tttagataca gatactaaga gaaaatcagc agaagaacga catcctctca aaaaaattgg
    29401 acaagcaaga gatattgcaa gcctcgcctg ttggttgttg acagatagta gtcaatttgt
    29461 tactggacaa gtctggaaaa tggatggtgg actcggaagt gtaaaaatca caccatagat
    29521 atatcatcta tcgtaaggaa ggatttaaaa atggtatttc tgctaaatat attttctaca
    29581 ttttttttga caggactcat ctggacgatt caaatcgttc actatccact ctttttaaaa
    29641 ataggtaaca atgaattttt agattatcac catggacatt cttttcgaat tagtttgatt
    29701 gtgatgccta tgatgttatt agaacttata tctacttttc tactttccat tgttgaaagc
    29761 aatgaatata aactatttca ctattcaggc ttgtttcttg ttctcatcat ttggctttct
    29821 acattttttt tatctgtgcc cgagcacaca attttaggag agggatggaa tgaaatgtct
    29881 ataaagaggt tggtgacaac aaattggatt agaactatcg cttggtctct tcattctctt
    29941 ttactcatta tagaattaag atccaagctt gttttataat ttaaaaaaaa acaatgataa
    30001 gagtaatcac gcaaagattg tttccaattg aaatttcttc aaagaataaa aatattttcg
    30061 agcgaaaatc tttttattct tttatcaaac ttactttccg agtatgtcgg ctgttttttg
    30121 catgatgtag tcttcaccac ttctctctgc aacttcaaaa ccatttttaa tttcatattg
    30181 tctgattgca ttcattgtag aagtatgaag ttgaccatcc tctgctcctg gatcaaaacc
    30241 tgcactttta agatttactt gaaatcgttt gattagagta ggagttgctt tttctggaca
    30301 aagaacattt ctaacatctg gcttttctgg agaaactagg actggcttag agattgtttt
    30361 ttggacgtca gggatgataa ttgtttttgt ataggatgga tattcaacag tcattgtaac
    30421 atttttatat acagcgggaa cttctttgca aaaaagaata tctccattgt aactacagga
    30481 attataagca ggtctttcta ataccttttc attgatagtc tctttttttc cttctattgt
    30541 ttctgtttta gaggagcctt tttctacttc agtggtagta gtttctttgt aaacgggtgg
    30601 agtcattcca acttgtcgat agcatccggg gctaacgtct tccggattaa tactcgcggt
    30661 tgttttttga tttctaagtt gtactattga attacaattt aaaataaaaa taattaaaaa
    30721 tagaaagttt atatgcttca tatagggttc ctttttaaaa catactgaaa aagccatgtt
    30781 taataggcaa atcaaaaaac atgaggaaaa ggatttaata agtcctccaa aagtaggctc
    30841 taataactat aactacgttt ctagttatta gagaaattta gattcatttt agaattttgt
    30901 ttcttttttt aatgtctagg aacctttttt tgtcttgacc tataccattt ctgcaaattc
    30961 taatcggata gtcacaaaaa ttcaatttaa aaaatcaatc aatcaagtag aggtttgaat
    31021 ggaagtatcg gtaataatca ttatagccat gtctatactc tctctcgtta ctgctgctgt
    31081 tttcacaagt aaagtgattt caattaaagt aggtggagag gatggaaaag atacaccaga
    31141 ggatagtaga ttaaaaacta tctcagctgc tatcgcagaa ggtgctatgg cttttttaat
    31201 tcgagaatac aaagttatta gtatttttat tgctgtcatg acagtcgtta tttttttcct
    31261 tttggataat ccaaagacac ctgatttcaa tgaaggtctc tttacagcaa ttgcttttat
    31321 atcaggagca attatctctt gtatctctgg atttatcgga atgagaatcg caactgttgg
    31381 aaacgtgcgc actgcacaag cagctaaaga atctatttca aaagcattta gaattgcttt
    31441 tgattctggc gctgttatgg gatttggtct cattggaatg gcagttttag gactgattgt
    31501 tctatttatc atttttactg gagcattccc atccgttgag aaacatattc ttatggaagc
    31561 acttgcaggc tttggtctgg gtggttcttc ggttgctctc tttggtcgtg tgggcggtgg
    31621 ta
//
LOCUS       DAOQOS010000062        31256 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_645_length_31256_cov_5.542547, whole genome shotgun sequence.
ACCESSION   DAOQOS010000062 DAOQOS010000000
VERSION     DAOQOS010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 31256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31256
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_645_length_31256_cov_5.542547"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1057
                     /locus_tag="PLF66_14315"
     CDS             <1..1057
                     /locus_tag="PLF66_14315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002978217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HMZ65267.1"
                     /translation="RALFADEMKGNIYTRFSNPNNSEFIEKVAYLEGAEDGIATASGM
                     SAVYTSLAGLLKSGDHILVSRSVFGSTHQILTQIFPRFGIESTYVDINKPETWEENIQ
                     KNTKMIFVETPSNPALDLIDLEWLGKLCKKYNLILNVDNCFSTPYIQKPIEYGADLVI
                     HSGTKYMDGQGRVIGGVIVGKKELIQDIRFFARHTGPSLSPMNAWVLSKSLETLAIRM
                     ERHSYNAFKLAEFLETEKDVKRVIYPFLPSHPQFNLAKKQMKLGGGIVTFLLEGGIER
                     GRKFLNSLELFSHTANLGDTRTIATHPASTTHSKLSDEERASVGIDAGLIRISVGLEH
                     IEDIIKEIKNALVESKK"
     gene            complement(1062..1592)
                     /locus_tag="PLF66_14320"
     CDS             complement(1062..1592)
                     /locus_tag="PLF66_14320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010418595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HMZ65268.1"
                     /translation="MIYKFNGITPTIAASAFIAPSAEIIGDVKIGENTSIWFQTLIRG
                     DVNYIRIGDNCNIQDMSLIHVARDVYPVNIGNNVSLGHRVTIHGATLKDFSFVGMGAT
                     IMDDVELGEYAFVGAGSLVTPGKKIPPGVLVMGSPAKIIRDINEKEREIITRTANNYQ
                     KYKDNYLNKENFIRLE"
     gene            1947..2495
                     /gene="lepB"
                     /locus_tag="PLF66_14325"
     CDS             1947..2495
                     /gene="lepB"
                     /locus_tag="PLF66_14325"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HMZ65269.1"
                     /translation="MHNYKSKSNLSDPVEFFKKLGIRVTISVLIAFLIAYLIKFVFFF
                     PFTVQNDFMAPEIKKDKTIYLTHLFQKDKILIGDIVLVRINENQVLLGRVMGRKGDRI
                     SIKNKKLYRNGNFIQESSDKIQFTDKRSPFASSFSKRDNLDEVLVKDKTYFLLVDNRD
                     DGIDSRQIGLISQDSIIGKLLF"
     gene            2637..3242
                     /locus_tag="PLF66_14330"
     CDS             2637..3242
                     /locus_tag="PLF66_14330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002997031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4254 domain-containing protein"
                     /protein_id="HMZ65270.1"
                     /translation="MNFVPEDVLSVFQKSIEDWHLKLAPSENPYNKDSMNHILYAKNQ
                     IDTIQWHVEDEIRRVDIPDSEIATLKRRIDALNQERTNLVEVLDDYFLNLYKNVTVQS
                     GARMNSETPAWLIDRMSILELKIYHMREQTERKDASESHINQCKNKLAILLEQRKDLS
                     TCLLELLDDLKKGVKFMKVYRQMKMYNDKDLNPALYANEKK"
     gene            3243..4298
                     /locus_tag="PLF66_14335"
     CDS             3243..4298
                     /locus_tag="PLF66_14335"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017214912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HMZ65271.1"
                     /translation="MNLLVMRFSAMGDVALLAPALIAISANYPNVQITLVTRGNFVPF
                     FYNMPNVNVIGMNLKKYKGLFGIWKFYKELEKLGPYDKIIDLHSSLRSHAFALIYRFR
                     GIPTFRINKGRKEKKEQIRKKNKILKNLPHTVDRYLKVFERAGFPAVTRRGPWINVDP
                     DSKVFAQDFFKRMGLKKKETLWVGFAPFAGHALKEWPMYKSINLVRLIQTEFNACIFL
                     FGSNEERKKLKVIQGDLENCFIVSGEKMGIRGELGIMEKLDLMVGMDSSNIHLMALLK
                     KPVIGIYGTTHPFSGFAPYGQEDSGVLQIENLPCRPCSIYGNTTCYRKDFACMEMIDP
                     ADVIKRIRFILNVNTLW"
     gene            complement(4545..5816)
                     /locus_tag="PLF66_14340"
     CDS             complement(4545..5816)
                     /locus_tag="PLF66_14340"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015682428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HMZ65272.1"
                     /translation="MRVMYFSDTFLPKVDGVALSIKNFSELLSDRGHEFLIACPKYGD
                     GDFSRVNDKIVVERFTCGYLPSYPDIKVVLPNPDKIKRLIKDFKPDLIHIHTPGLLGQ
                     YAISAAEKYGIPTIGTYHTLMAEQNTYVSLYRLLKLDKLFMKINRFDKELKLKDLIKV
                     QKWDNFNIRKKIILKLCNNLYDRCDLIISPSNLLREQLLEYEIQKPITVVSNGMDLSR
                     FKGTVKELPANPRLLHVGRISYEKNCDVIINSFKIILEKLPTATLTIIGDGPAIATLK
                     HQAEKMKLEDKIIFAGFIDNSKLHEVYPQYDLFLTASTMETQGLVILESIACGLPAVG
                     VKSFAIPELIKDEKNGYCAEPFNVVEIADRCVEILTDKEKYKKFSENSIKIASEHELN
                     GCVLKMEDVYERVIILHKDKEKKDHLLNLFM"
     gene            complement(5880..7013)
                     /locus_tag="PLF66_14345"
     CDS             complement(5880..7013)
                     /locus_tag="PLF66_14345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65273.1"
                     /translation="MRKIKLLFYFILFLSFSIFAQEESRYIYIEWEKIPKAKEYQIQI
                     SDSVKFFSILYTEKTKETSVKIEPNARYKYGRIAAIDDLGNKGKPSDIFEIESRIMEK
                     RKPNALHSDLSNYLTANHLIQLDSSDNKSKGFVTYYKINDGKWLNYQGGIQLQKEGKN
                     TIQYYSIDLLGNRERMKAVEYILDTEGPLAEFSISDTFQDSEKFLYTNKNSKINLSIQ
                     DALSGIQSANVFLRTANESIEIDWNEKESISIPEKFSDRTVELFIVATDRIGNTKTYS
                     KFFRHDIKTPEVSSDIFPKKNTEGRLSYITQIQAKDTCSGIQGIFYSINNGPFEVYSE
                     PFTISEVGEYELKIYAIDNAGNKSPIQYERIKISEIQKKKPVK"
     gene            complement(7194..7853)
                     /locus_tag="PLF66_14350"
     CDS             complement(7194..7853)
                     /locus_tag="PLF66_14350"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HMZ65274.1"
                     /translation="MSVKTLETKTMRVIIADDHELIRRVMAVIFQKMPNIKIVGEAGN
                     GEELFSLLKETPCDLLCLDVYMPSFNFFSAIPIYKKLNKKMKILVLTSMDDHFSIKKI
                     IKEDIDVIVSKSEPAQVITDAVNAVEAGKRFFSYVIQDIINSEYVQSLSPNKLIDVLT
                     PQEKKVMSFVARGFKNKEIARELNVQEFTIDFHKKNIKTKMNVSSNAEITKIAIEHNL
                     I"
     gene            8538..8696
                     /locus_tag="PLF66_14355"
     CDS             8538..8696
                     /locus_tag="PLF66_14355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010573318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65275.1"
                     /translation="MSDNHNPQGYWTFLVILSLNILFFVYISFIHPGVEPVDKIKASN
                     SVKQEEKK"
     gene            8787..9647
                     /locus_tag="PLF66_14360"
     CDS             8787..9647
                     /locus_tag="PLF66_14360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002755275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HMZ65276.1"
                     /translation="MILKCLKTYLVFSFLFHFFSMNLFSYDPSYTQNNNEMPSEIVDM
                     KVVEKLGKNINLNLKFKDENANEVTLQNYFKSDKPVFLTIIYYKCPTLCNFHLNGISK
                     VFKSLDWSVGKEFEFVAVSMDPKETPELAAKKKDAYLTDYKKSGTSRTNDGWHFLTGE
                     EPQIKELADSLGFKYKWNAPIKQWIHPAVAYIITPEGKISRYLHGIEFGERDLKLSLV
                     DASNGKIGNFIDKFALFCFQFDPNKNKYTLYAYNIMRIGGAITVVILGAFLFAFWRLQ
                     MKNTNTKGEF"
     gene            9648..10619
                     /gene="coxB"
                     /locus_tag="PLF66_14365"
     CDS             9648..10619
                     /gene="coxB"
                     /locus_tag="PLF66_14365"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010577229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HMZ65277.1"
                     /translation="MSWLNIHLANTFMPVEGTDLAGGVDNLYIFLLISSLIAFVILIG
                     GMTGFIIKYKRKSANDKTAYITHNNVLEFLWSFIPFVIMMVIFYWGKILYDEYRTVPE
                     DAEEIHVTGRQWVWRFAYKNGYAFSTKPVSDAMLNVPVNKPIKLVMTSGDVIHSFYIP
                     GFRNKQDVVPGRYTYIWFKATKVGQFQVFCTEYCGLEHSNMPAMINVMPQAEYDAWFK
                     AYVPEASAGGLADKGKELFGERGCTACHSINGTRIVGPTMKGLAGSKREFSDGTSAVA
                     DDAYLRESILNSAAKIVKDYPAAMPIFKGQIEEEDVSAIIDYIKSLK"
     gene            10628..12226
                     /locus_tag="PLF66_14370"
     CDS             10628..12226
                     /locus_tag="PLF66_14370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HMZ65278.1"
                     /translation="MAHATATDTGHDYLSHQKGLWSWLTTIDHKRIGIMYMIVILFFF
                     LIGGIFALLVRMELFSAGPTLGIDSGTYNRLMTFHGAIMVFMVIVPGIPSIFGNFLIP
                     IMLGAKDVAFPRLNLASWYIFMAGAITAFASLFFGGADTGWTFYTPYSIRQPTGVIFV
                     VLGAFTMGMASILTGLNFIVTVHKLRAPGQTMSRIPLFVWSTYATAIIQVLATPVLGI
                     TLLLLVAEKTLGVGIFDPTIGGDPVLFQHFFWFYSHPAVYIMVLPAMGIISELVATFS
                     KKTIFGYTAIAYSSLAIGAVSFIVWGHHMFVSSQSEFAGIMFSVITMLVGVPTAIKLF
                     NWIATMYMGSIKLDAPMLFAIGFMFLFTIGGLTGVWLATLGMDVHFHDTYFVVAHFHY
                     VMVGGTLMAFTGGIFYWFPKMFGKVCNDSIARISWVFIFTGFNVTFFPQFILGAMGMP
                     RRYFDYVPQYESLNRISTIGSWLIGLGFLVALFAIIQGLLKGEKASNNPWGGTTLEWQ
                     ATSPPHHENFEKTPIVSVGPYEYR"
     gene            12213..12938
                     /locus_tag="PLF66_14375"
     CDS             12213..12938
                     /locus_tag="PLF66_14375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002974686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3 family protein"
                     /protein_id="HMZ65279.1"
                     /translation="MSTVSSESHFHHAHHFDSAEHQYASAKQGVWLFMVTEILMFGGL
                     FVAYLIYKSLYAPVFHVGSSYLDVKMGTINTFLLITSSFTMALGIYFNQKNDRKKAVL
                     FLALTILCALGFMVVKFFEYSHKIHLGLVPGKYFNNPEFVQKVADFANNMQAQSSAHQ
                     DMDIAFLKKIAPLYFGFYFVMTGLHGFHVLVGACLILWVLIKTAKGNFSSEYYTPVEG
                     VGLFWHIVDLIWIYLFPLLYLVG"
     gene            12948..13550
                     /locus_tag="PLF66_14380"
     CDS             12948..13550
                     /locus_tag="PLF66_14380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016546470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HMZ65280.1"
                     /translation="MEEFVNYGLYFIACVCVLVPLFGLGLAPGIIVNLEVAAILGTLY
                     AQIVEKGLIKTLIKDKGDTALIKPMVPTLQNIQNTIDSIESGDMGKKEEKHDHEDHGH
                     HIIPIPVYAGVLGILVLGTIITVGVAQIDFGAWNTVIAMFVATIKASFVLLYFMHLKY
                     DNAMNRVIFGSGFFFLLILFGFSIVDILTRTKLLLGFTKF"
     gene            13550..13750
                     /locus_tag="PLF66_14385"
     CDS             13550..13750
                     /locus_tag="PLF66_14385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016545845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65281.1"
                     /translation="MSLATCKCNTDRNSPNAEEIRKYSKMSMFLLLFGVSAVPVSLEY
                     KCKKCGEIFDRLSEEELKNYKY"
     gene            14490..19061
                     /gene="gltB"
                     /locus_tag="PLF66_14390"
     CDS             14490..19061
                     /gene="gltB"
                     /locus_tag="PLF66_14390"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HMZ65282.1"
                     /translation="MHNNEQNLSPDSHLPKAQGLYDPSYEKDACGVGFVAHIKGKSSR
                     DIIDKGIRLMCNLEHRGAEGAEPKTGDGAGIKIMIPDKFFRKVLPFSLPPFGEYAVGM
                     LFLPQDPLIREGVETIIEKVIIDEGEHCLGFRDVPVNPDVVGTVARKTIPVFRQVFIG
                     KSQNTEAGDAFERKLFLIRRIIDRRFRTEYKLDRSQYYVPSFSSRTIVFKGMLLGNQV
                     KPFYLDLSSPDMESSFCLTHTRFSTNTFPTWDLAHPYRLIAHNGEINTLQGNINWMAA
                     RQIVMESPYYGAELKRMLPIIMEGQSDTATFDTVLELLCMGGRSLPHAVMMMIPEAWS
                     KNTAMDPDRRAFYEYHATLMEPWDGPAAIAFCDGKVIGATLDRNGLRPARYKITKDDI
                     VILSSEAGVLNTEPDMVLKQGRLRPGRMLLIDLEKGQILDDEAIKREISTQKPYRKWV
                     EENMVRLNQLPDAAGTKKEENHNTIIEQMRAFGYNSEDLLTTMKPMALNGEEPTGSMG
                     SDVSLPVLSDKPQPLFRYFKQNFAQVTNPPIDPIREELVMELTTYIGPEGNLLDETPE
                     HCHRLELEHPVLTNDELEKIKRINQGSLKAITFSILFDPNKKHDMRHSLNQVCQKAAD
                     SVRAGYNLIIISDRGVSKDKAAIPSLLAVSGIHHYLIRAGLRTKTGLILESGEPREVS
                     HFALLCGYGANAVNPYLAFDALNDSIREGVFSDIPDYKTAQKNFIKSIGKGLFKVFSK
                     MGISTLQSYCGAQIFEAIGLDLELVSTYFTGTATRIGGLSLEMLEEETVRRHKDAYDP
                     TFFPGGLEPGGVHYYRKTGDPHLFNAQTIHKLQQATIKGDYKIYKEFSKLIDDQKEKQ
                     ITLRSLFQIDEKKCKPIPIEEVESVSEIVRRFQTGAMSFGSISHEAHSTLAIAMNRIG
                     AKSNTGEGGEDEERFKTLPNGDSMRSAIKQVASARFGVTTNYLVNADDLQIKMAQGAK
                     PGEGGQLPGHKVDKNIARLRFSTPGVTLISPPPHHDIYSIEDLKQLIFDLKNVNPRSR
                     VSVKLVSEVGVGTVAAGVAKAHADHILISGHDGGTGASPLSSIHYAGTPWEIGLAETH
                     QTLVINGLRDRVYLAVDGKIMTGKDVVIGALLGAEEFGFSTSALITIGCVMMRKCHLN
                     TCPVGVATQDAHLRTKFTGKPEYVINFMHFIAEEVRELMAQMGFRTFTEMIGQVEMIR
                     FDRPKNHWKARGLDFSKVLAKPKPAHFPTEMYRTKEQNHGLERQMDNEIIRRSRAAID
                     YKQKVKFDMKIINLNLSVGTMLAGEVARRYGELGLPEDTIDITFRGNAGQSFGAFITN
                     GITLRLIGDSNDYVGKGLSGGKIIVAAPQEAAYDPTENIIVGNTCFYGATKGTAFING
                     FGGERFAVRNSGAKIVVEGIGDHGCEYMTGGSVIVLGKTGRNFGAGMSGGTAYVWDPD
                     GSFPKNVNLQMVELEKLSDPEEIAQVFKLITLQKEYTNSKVATNILANWKTEVEKFVK
                     VMPTDYKKALLKLKEEAANNINTKEASHG"
     gene            19054..20493
                     /locus_tag="PLF66_14395"
     CDS             19054..20493
                     /locus_tag="PLF66_14395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015679307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HMZ65283.1"
                     /translation="MGKPTGFVEYKREGLKYESKETRIQNYKEFEKPFEEGVAKVQAA
                     RCMDCGIPFCHSETGCPVDNLIPEFNDFVYKGKWKEAAENLHSTNNFPEFTGRLCPAP
                     CEGACTVGLIDQPVSIKAIERTIVDRAFEEGWVGPKPASSKSGKKVAIVGSGPAGLAA
                     AQQLARAGHDVVVFEKADRIGGLLRYGIPDFKMEKWHIDRRVKQMEAEGVVFKTNVHV
                     GKDIQAKELLSGYDSVILAMGAEKPRDLNVPGRELKGVHYAMDFLTLNNKKVAGDSVD
                     YISAKDKHVIVIGGGDTGSDCVGTSNRHGAKSITQIELFPEPPKDRDPSTPWPLYPKM
                     LRTSSSQEEGQVIRKWSINTLGFRGNEKGEVTAILGNQVRFENGKFEPIPGTEFEWPA
                     DLVLLAMGFVSPVKEGLISQLMEQGLELDKSGNVKASFGLKNGAFATSIPKVFACGDV
                     RRGQSLIVWAISEGRKCADQVNHFLSTKV"
     gene            complement(20774..23239)
                     /locus_tag="PLF66_14400"
     CDS             complement(20774..23239)
                     /locus_tag="PLF66_14400"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HMZ65284.1"
                     /translation="MIQFIFILFISLSGILAQEKAVDPDPKSENTNNQIEKPKQSKDE
                     HIEENFFKIEENIVVTASRTKEKLKNAPASMVIITEEDIRNRGYQSIDEILYDLPGFD
                     LSFSNGIPYIMGYQRGYRTPFMSRTLFMIDNKIQNDMYTQEAELSRQIPMTNIKRIEV
                     LYGPASAVYGANAFQGIINIITHDGTEPRKMKSSEIEKSSYSKISLQGGENNTKGVDA
                     SSTIRIGEWAFAASGKLFSSNEPDLSNRSGFNSNYWYGNPTVWGGVRYYQNDGVNLSR
                     YHDPTKNYGLEASVSNGGLKIGTIAWSRKEGYGVKYPGDRAQNNSFWEVMSRQVYIEY
                     TSNVSSNLTTNTLFTFRESAFRGPWAEAEPDTKKGKEMYSYLSQTYWGNQNKSFLLNH
                     NIDYKISNSILLTTGLKVEKRDMTKQYDIPGYWPGSYDSWNRYEGKQALDLTPYFPNG
                     YAVQYSDQPIYYKYADGKTNMPKDNIVQVYDLGGFAITTYEIGKFRFSPGIRYDYNSI
                     YGQSINPRASAIYKYSSTGTIKLLYGEAFQEPPFILLYGGWSGRQANNKLKPEKERTT
                     ELIIMKQFSSVLSELSFYYSRYENVIKETARNEGSRKIYGFEFRNKIQLNNPIPNSNK
                     LDLYFYYTFTEAISESYYNFNAPRTAWTPQNTNPVDWISGTTVLGKYENDFYAANPTA
                     PPLPRKKSYHTLGDISPHKISAGINLPIQHILNINLRVNYVGPRQLYLSNELRNKGMP
                     FERDKGIILNNYFLFNAAFSFNLKYTTFVLKVFNLLNHSFLHPGQEAADAGNNYYERS
                     NGYRNSILAQPGRYYLFSLNFEF"
     gene            23846..24715
                     /locus_tag="PLF66_14405"
     CDS             23846..24715
                     /locus_tag="PLF66_14405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001053467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HMZ65285.1"
                     /translation="MNKPITGIIAAQRTAEGEGFIVNRPFPTKFLNFYDPFLLLDEMG
                     PTIYEKGEAKGAPDHPHRGFETVTYMIEGEGEHEDSAGNRGIIEAGGVQWMTAGKGVI
                     HSEMPSRKIQEEGGKVHGFQLWVNLPSIAKMIPPKYQEKKSSEIPIYEGEGLWVKIIA
                     GELFGVKAEIHTHTPILYWHVRLSKNKKIELPVPIDFNVFLYLIEGKGIFEANGQTLS
                     GKQMIQFSNSKGESIYLENPSGENLDILVLGGKPLNEPVARYGPFVMNTQKEIEEAFS
                     DYRNGNMGSIDRP"
     gene            complement(24718..25809)
                     /locus_tag="PLF66_14410"
     CDS             complement(24718..25809)
                     /locus_tag="PLF66_14410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65286.1"
                     /translation="MWNRIKVLMLFLFTLSILSEDILDIDNPTITWSASEGSTEYILQ
                     IKNEVDEITYEIETKETYYKVDLEPGRYSHRVGTYNKFGKVTSYTDWVPFIVVRSLPP
                     IVTSEQFFISSKSEKEKTILIEGKNLYKDTKVFLKNENSTVQLKNKVFKKGTIEITID
                     NENMKEGSYDLILENPRKKILTLNQFYQLGETKSLPNSSRENPYPYWKEAGKSMLIPG
                     WGQYSKEHFPSSIFFNLCFIIAGANYSYSYQTFHSDKKKYNNTINQGILYQMIDKQDS
                     VLLYNYFNSNEYFKRGEDSANKVYTASILFASIYTLNILDALLWKTSISLTKQEATLN
                     LYTKVYSNPTNLNITNQNSYIEFGFRFVF"
     gene            complement(25799..27916)
                     /locus_tag="PLF66_14415"
     CDS             complement(25799..27916)
                     /locus_tag="PLF66_14415"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HMZ65287.1"
                     /translation="MDLKKDKSYLVILLLLLIILIASVLLYKNIHSGATGNDNEVVGT
                     LTFKHKTIERKYDTDVIWENIESGLQIRNKDTIRSGSFSDAVLTLKDKTKININENSM
                     IYIDMNEGDINLNFAYGSMSLSKRVDGYSDTTLKIKSGNNTLEVKNSDLTLEKKSKEE
                     LTFQVNQGTAKLKTGNQEKEIKENESAKLNKAEIEISEVNLSLQSPADGTILTDKKEN
                     PSVTFSWNSKNAKNLKIELAYDSRFQNIYQSINAENNSIALNLQQGTYYWRISSEKDK
                     IKKRSIREYSAFRKITIFTVTSPILLSPTKNQTFNFTTVSPIIPFSWNKLETARNYKL
                     DISRKSDFSEIIKSVTTNLTSLGVDNLEPGKYYARISIDPIREEFKVDPSKTISFSIE
                     KKNDLESPQLISPSDGQSITSIQQDKSEIILLAKDLAEINRYNFQVSKNNNFSALTIN
                     ETVTANQIRLNKKLELGTYFWRVIGISQDGKNTPPSEIRKFSIIENQPIELISPKSGS
                     EFDMKDNPVVFRWKKLPVQAEYLLEVSASKDFQEKIHSIKTQELSNSFSISTEGTFFW
                     KVTAFSDDGSILSKSAILSFSIEEVEILKPIFPTRNERVDMSPLDSLTLRWEKTKKDT
                     RYEVELYNNKNGEKTLIAKSKTKNSSYQIKDLSKLDEGNFIWTLQDLSKDKSNKVVIP
                     FKIYLSEKPKVPEIRTPKKFYVE"
     gene            complement(27920..30244)
                     /locus_tag="PLF66_14420"
     CDS             complement(27920..30244)
                     /locus_tag="PLF66_14420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004451977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HMZ65288.1"
                     /translation="MEENKPEKNRIQYTPDFTMDAIPILDINTSITEIHKSIENNLEP
                     NSDETSKEVQKPKLVDEKTKGDELSSNSSIEKNPKPKTQKTGFLAKMENLRETPSTST
                     EKVNSDTATPDKPTSKAKYSLQLKMMLVISIVIATTVSVMIALATYFFKDDNQKRIDQ
                     NNLDIVRIINSKVVTDLVAEINKGKILALTLKQEFKSNSQKRFFIEQYFKNDNSFLFL
                     GVYKKENGKMETVDSIFNQEYLKKVSLTEEDVISPIHQNLKYFSKSFEGVPTLLNTSP
                     GTQETTISISIPLEEGEKSEYILVILLRLEKILDAFKKSGINTTYMVNEDGMVLAHPD
                     PKIISSGKNFMTTPIVRQMLTSQAKNELKPFQDTDGKKYLGAFQKLGFANAGIISVVE
                     EDLAYKAVYQIQQRNILIMIISICVSLIIVFIFAKSISNPLLKLLDETIKISKGIFQL
                     EIKRTTNDEVGVLTDYFQSMAKGLEEREKVKSMLGSMIDPIVVSEGMRDLAALKRGDE
                     KMITAFFSDVAGFSTISEQLNSVQLAGLLNEYLSAMTIILKNNEGVLDKYIGDAIVGI
                     FGAPVPVNEHYIKAARASLEMIHKLKDLREYWTKNNLYSKDAQEMDIRIGLNTGLAKV
                     GFMGTDAMGSYTMMGDTVNLAARLEAAAKDYGVNILISESVKTEIEKEMFTRELDLVR
                     VKGKNEPVKLYELISNHTDVSSNIKEATGLYEEGFKAYLRKEWLKSIQFLQQSEKAKG
                     KKDKACRQLIERCEFYKTNPPDSNWDGVYTRTHK"
     gene            30456..>31256
                     /locus_tag="PLF66_14425"
     CDS             30456..>31256
                     /locus_tag="PLF66_14425"
                     /inference="COORDINATES: protein motif:HMM:NF017183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig domain-containing protein"
                     /protein_id="HMZ65289.1"
                     /translation="MKTNLFIIGTIILLAQCRPNLDKSEIPSISYLLYKQNKNTNTLS
                     FAYPSQSYSFLVNVPIATQSPVASTVFDSYSVSPALPLGLIFDTKTGQISGTPTTASP
                     SSSYTITATNSQGSATFTISITVTNSPPTGLAFTGSPFSWTENQSITVTPVVVGVVTN
                     CISTPNLPTGLTISSSCVISGIPTIVQGITTYTITASNIYGSISVAVDIAILASPIKR
                     IFVTNSGYTPNVAFNNLATADNICNTDSAKPAGLGTYAAMIAIPLLRVA"
BASE COUNT        10013 a         4842 c         5675 g        10726 t
ORIGIN      
        1 aagagcttta ttcgccgatg aaatgaaagg aaatatatat actcgatttt cgaatcccaa
       61 taattctgag tttattgaaa aggttgctta tttagaaggg gcagaggatg gaattgcaac
      121 tgcttctggg atgtcggcag tttatacaag ccttgctggg cttttaaaat ctggtgatca
      181 tatccttgtt tctagatctg tttttggttc tacacatcaa atcctgacac aaatatttcc
      241 tcgatttgga attgaatcta cctatgtgga tattaataaa ccagagactt gggaagaaaa
      301 tatccagaag aatactaaaa tgatttttgt agagacacct tccaatcctg cattggattt
      361 aattgattta gagtggctcg gaaaactttg taaaaaatac aatttaattt taaatgtaga
      421 caattgtttc tccacacctt atattcaaaa accaatcgag tatggtgcag accttgtgat
      481 ccattctgga accaagtata tggatggaca gggtagagta attggtggag tcatagtagg
      541 aaaaaaagaa ctgattcagg atatacgttt ttttgcaaga catacaggac cttctttatc
      601 accaatgaat gcttgggttt tatctaagag cttagaaaca cttgcgatta gaatggagcg
      661 tcattcgtat aacgcattta aattagcaga attcttggaa acagaaaaag atgtgaaaag
      721 ggtaatctat cctttcctac cttctcaccc tcaattcaat cttgcgaaaa agcaaatgaa
      781 attaggtggt ggaatagtta cttttctatt agagggtgga attgagcggg gtcggaaatt
      841 cctgaattcg ttagaactat tttctcatac tgccaatctg ggggatacta gaacgattgc
      901 tacccatcct gcatccacaa ctcactcaaa actttcggac gaagaaagag catccgtggg
      961 aatagatgct ggattaattc gaatctcagt tggtttagaa catattgaag atataataaa
     1021 agaaattaaa aatgctcttg tagaatctaa aaaataagag tttattctaa ccgaataaag
     1081 ttttctttat ttaaataatt atctttatat ttctgataat tgtttgcagt tcgagtaatg
     1141 atttctcttt ctttctcatt tatatctcta atgatttttg caggtgaacc cattacaaga
     1201 actcctggcg gtattttttt tccaggagtt actagggaac ctgcacctac aaatgcatat
     1261 tcgcctaatt ctacatcatc cataatagta gctcccattc cgacaaaaga gaaatctttg
     1321 agagtggctc catgaattgt cactctatga ccaagagaaa cgttatttcc tatattaact
     1381 ggataaacat ctcttgcaac gtgaattaaa gacatatcct gtatattgca attgtctcca
     1441 attcgtatat agtttacatc gcctcgaatt aatgtttgaa accaaatcga tgtattttcc
     1501 ccaattttta catctccaat aatttctgca cttggagcaa taaatgcgga tgcagcaatc
     1561 gtaggagtaa ttccattgaa tttatatatc atattaaacc ttaaaagcag gattgatgat
     1621 tcattaaaat ttgtaaattc taatctattt gaaaagaaag gagtaaattt ggagaataaa
     1681 aatgagcgat cgaaaaaagg tagctagaat cagaatgaat atcttttgat ctacattttc
     1741 cgcgaaacgg atttatattc agaaataact gattcgatag aaagctgata gcattgttat
     1801 aaaatgtttc atttgaaatt attaaagatg gcaattcatt ggtataagaa taaagaagag
     1861 cgcattactc tagatttgtt attggggaaa tttcagtttt ttttatcttg ctgatttcaa
     1921 aaagaatttc ttattggttt atctagatgc acaattacaa atcgaaatcg aatctttctg
     1981 atccagttga attttttaaa aaattaggaa tacgagtaac tatttcagtg cttatagcat
     2041 tcctgattgc ttatttaatc aaatttgttt tcttttttcc atttactgtt caaaatgatt
     2101 ttatggctcc tgaaataaaa aaggacaaaa caatttatct aactcattta tttcaaaagg
     2161 ataaaatact aatcggagat atcgttttag ttcgaatcaa tgaaaaccaa gttttactcg
     2221 ggagagtcat gggtaggaaa ggagaccgta tttccattaa aaataaaaaa ctatatcgaa
     2281 atgggaactt tatacaagag agttctgata aaatacagtt cactgataaa cgttccccgt
     2341 tcgcaagctc cttttctaaa agggataatc tagatgaagt tctagtaaaa gataaaactt
     2401 attttctact cgtagacaat cgggatgatg ggatagactc aaggcaaata gggctcatct
     2461 cacaagattc aattattgga aaactcttat tttagaactt gcatttattt aatcctacaa
     2521 aaaaatataa ttaaatcgtt attagatttg taaagaaaaa gaaactaaat ttgttttgta
     2581 tttatcgtta tttaatttct ctgattacga tttatcttta ttaaaaggca tttagcatga
     2641 actttgtacc agaagatgta ttatctgttt ttcaaaagtc gattgaggat tggcatttaa
     2701 aattagcacc atccgaaaat ccatataaca aggattcaat gaatcatatt ttatatgcaa
     2761 agaatcaaat tgatacgatt cagtggcacg tagaagacga gattcgcaga gtggatattc
     2821 ctgattcaga gattgcaact ttaaagagaa ggatcgatgc actgaatcaa gagagaacta
     2881 acttagttga agtactagac gactattttc taaatctata taaaaatgta actgttcaat
     2941 caggtgctag aatgaattca gaaacgccag catggttgat tgatcgtatg agtatattag
     3001 agttaaaaat ctatcatatg cgagaacaaa cagagagaaa agatgcgagc gaaagtcaca
     3061 tcaatcaatg caaaaataaa ttagccatct tattagaaca aagaaaagat ttatctacct
     3121 gtttattaga acttttagat gatttaaaaa aaggtgtcaa gtttatgaaa gtctatcgac
     3181 aaatgaaaat gtataacgat aaagacttaa accctgcact ttacgcgaac gaaaaaaaat
     3241 aaatgaactt attagtaatg agattttctg cgatggggga tgttgcactt ctcgcacccg
     3301 cattaattgc tatatcggct aactatccga atgtgcagat aacccttgtt acccgaggca
     3361 actttgttcc ttttttttat aatatgccaa atgtaaatgt aattggtatg aatttaaaaa
     3421 aatacaaagg tttgtttggt atatggaagt tttataaaga attggaaaaa ctaggtcctt
     3481 atgataaaat tatagattta cactctagtt taagaagtca tgcctttgca ctcatctatc
     3541 gatttagagg aattccgaca tttcgaatta ataaaggtag aaaagagaaa aaggaacaaa
     3601 ttcgcaagaa gaataagatc ttaaaaaacc taccacatac agtggatcga tatttaaagg
     3661 tgtttgagcg agcaggtttt ccagctgtca caagaagagg tccttggatc aatgtagatc
     3721 ctgattctaa agtttttgca caagattttt ttaagagaat gggtttaaag aaaaaagaaa
     3781 ccctttgggt tggttttgcg cctttcgctg gtcatgcatt aaaagaatgg ccaatgtata
     3841 aatcgatcaa tttagttaga ttgatacaaa ctgaattcaa tgcttgtata tttttatttg
     3901 gttctaatga agagcgaaaa aaattaaaag tgattcaggg agatttagaa aattgcttca
     3961 ttgtctcagg agaaaaaatg ggaattcgtg gagagcttgg gattatggag aagttagatc
     4021 ttatggttgg catggattca tccaatattc atttgatggc tcttcttaaa aaaccagtca
     4081 tagggatcta tggaacaacg catccttttt ccggatttgc tccctatgga caagaagatt
     4141 ctggggttct acaaattgaa aatcttccat gcagaccttg tagtatctat ggaaacacta
     4201 cttgctatcg aaaggacttt gcctgtatgg agatgatcga tcccgcagat gtaatcaaac
     4261 gaatccgatt tattttaaat gtaaatactt tgtggtaacg attcatccaa atcatgaaag
     4321 ccaattcttt cagttgtaag aaagaaaaaa gattgcaaga gatggtcagt gagaatgcgt
     4381 tggtcgctaa agctggcagg gatggaaaca gcaaatacag ataaaagatt cgatacccga
     4441 tacttgttgt tgagagatgt tgattaaatc attttcttct tttggtatag agactgtgag
     4501 caagattttt aaatctaact cactttgctc tataaacaaa aagattacat aaaaagattt
     4561 aagagatggt ctttcttttc tttgtcttta tgcagaataa taactctttc gtaaacatct
     4621 tccatcttta gaacacatcc atttagttca tgttcggatg caatcttgat cgaattttca
     4681 gagaattttt tatatttttc tttatcagta agaatttcta cacatctatc tgcaatttcc
     4741 actacattaa atggctccgc gcaatatcca ttcttttcat ctttaattaa ttcaggaatt
     4801 gcaaaggatt taacaccgac agccggtaga ccacaggcaa tcgattctaa aattacaaga
     4861 ccttgtgttt ccattgtaga agcagttaag aataaatcat attgtggata gacttcatgt
     4921 aattttgaat tatcaataaa acctgcaaag ataattttat cctctaactt cattttttca
     4981 gcttgatgtt tgagtgttgc aatagcgggt ccatcaccta tgatagtgag tgtagctgtt
     5041 ggtagtttct ctagaataat tttaaatgaa tttatgatta catcacaatt tttttcataa
     5101 gaaattcttc ctacatgaag caaacgagga tttgccggta gttctttcac tgttccttta
     5161 aatctggata agtccattcc atttgaaact acagtgatcg gtttttgaat ttcgtattcg
     5221 agtaattgtt ctcttaataa atttgatgga gatatgataa gatcgcatct atcgtataga
     5281 ttgttacaaa gctttaagat gatttttttt ctaatattaa aattatccca tttttgaacc
     5341 ttaatgagat cttttaattt gagctcttta tcaaagcggt tgattttcat aaataactta
     5401 tctagtttta aaagtcgata taaagacaca tatgtatttt gctctgccat gagagtgtgg
     5461 taggttccaa ttgtaggaat tccatatttt tctgctgcac taatcgcata ttgtccaagt
     5521 aatccaggag tatgtatgtg aataagatct ggtttaaagt ctttaattaa tcgtttaatt
     5581 ttatctggat ttggtagaac aactttaata tctggataac ttggaagata accacatgta
     5641 aatcgctcta ctacaatttt atcatttact ctggaaaaat ctccatctcc atatttaggg
     5701 caagctatca aaaactcatg ccctctatct gataatagtt cagaaaaatt ttttatagat
     5761 aaagcaactc catctacttt aggtagaaaa gtgtcagaaa aatacattac tcgcatcgaa
     5821 ttaggctcct tagttactgg tcatttttta attttataat ttcattcaca atcaaaaact
     5881 tattttacag gtttcttttt ttgtatttcc gaaattttga ttctttcata ttgaattgga
     5941 gatttatttc ctgcattgtc aattgcatag atttttagtt catattctcc aacttctgaa
     6001 atggtaaagg gttcagaata gacttcaaaa ggaccattat tgatagaata gaaaatgcct
     6061 tgaattcctg aacaggtatc ctttgcttga atctgggtaa tatacgatag gcggccctct
     6121 gtatttttct tggggaatat atctgaagaa acttctggag tttttatatc atgtctaaag
     6181 aacttgctgt atgtctttgt gttaccaatt ctatccgttg caacgataaa tagttccaca
     6241 gttctatctg aaaatttttc cggaatcgaa atactttctt tttcattcca atctatttca
     6301 atggattcat tcgctgttcg taaaaaaaca tttgcagatt gaattccaga cagagcatct
     6361 tgaatgctaa gatttatctt agagttttta tttgtataga gaaatttttc agaatcttgg
     6421 aatgtatctg atatagaaaa ttcagctaat ggaccttctg tatctaaaat atactcgact
     6481 gctttcattc tttctctgtt gccaagtaaa tcaatagaat aatattgaat tgtattttta
     6541 ccttcttttt gtaattgaat tccaccctgg taattgagcc atttgccatc gttgatttta
     6601 tagtaggtaa caaacccttt cgacttatta tcagatgaat ctaattgaat taaatgatta
     6661 gctgtgagat aatttgataa atcagaatgt aaggcattcg gttttctttt ttccatgatt
     6721 ctagattcaa tttcgaaaat atcagaaggc ttacctttat ttcccaaatc gtcgatagct
     6781 gctattcttc catatttata tcttgcattt ggttctatct ttacggaagt ttcttttgtt
     6841 ttttcagtgt aaagaatact aaaaaatttt actgaatctg aaatttgaat ttgatattcc
     6901 tttgccttgg gaattttttc ccactcaatg tatatatatc tactttcttc ttgagcaaag
     6961 attgaaaaag aaaggaatag aataaagtaa aaaagcaatt ttatttttct catttaaaat
     7021 ttcttcctat aaatatttag aacattcatg acagaatgtt tttttttaag atttcgtata
     7081 gcaaaaattt aatttataga ttctggtttg aatgcgtttt ttagtgaaga attgagtggg
     7141 ggagtggggg gtaataaagg taagccgcaa gaggcgactt acctttatgt gatttagatt
     7201 agattgtgct caattgcaat ttttgtgatt tctgcatttg aacttacatt catttttgtt
     7261 tttatatttt tcttatggaa gtcaatggta aattcttgta cgtttagctc tcttgcgatt
     7321 tctttatttt taaaacctct agctacaaaa gacattactt ttttctcttg tggtgttaga
     7381 acatctatta gtttatttgg gctgagagat tgaacatact cactgttgat gatatcttga
     7441 atcacatagg agaagaaacg ttttcctgct tcgacagcat ttacagcatc tgttattact
     7501 tgtgcaggtt cacttttaga aacgattaca tcgatatctt ctttaatgat ttttttgata
     7561 gagaaatgat catccattga ggtgagaact aggattttca tctttttgtt taattttttg
     7621 taaattggga ttgctgagaa gaagttaaaa gatggcatat atacatcaag acatagtaaa
     7681 tcgcatggtg tttcttttaa aaggctgaat agttcttctc catttccagc ttctccaact
     7741 attttaatat ttggcatttt ttggaaaata acagccatta ctcttcgaat gagttcgtga
     7801 tcatctgcaa tgattactct catagttttt gtttctaagg ttttaacgct cataaaagtt
     7861 tgtaagtgca atagcactta tctcctgtaa atttttttag acctattata aatatctgta
     7921 caccttaatt tttcgtaaac ccttttattc aaaaaaactc tatttttcaa attttattta
     7981 ctaggtcaat gtccatatta ctatagtata atcctatgaa ttatataaga atgtgtccaa
     8041 atttaataat taactgctat cagaaaagaa aaaatgtttt tattgagttg gagttttttt
     8101 taaaacaatt gctagtgttt ttaaaataaa gacacttaaa tgagttaaga aagtttaatt
     8161 aaaaccttca gggaaataga gggaatgaat gaaaatacta ttccgaatga tttctgaaag
     8221 caatcctaat gctaaacatg tgaaaataga attcttggct aaaccaattt tttgagtttg
     8281 agctgattga agttcaggtg aagaaaaaat gtatcggtag attcttggaa acttattttt
     8341 gaaatcaaaa cacattcatc caccaatcgg tttttatgaa aagctctatc cgaatagaat
     8401 cctagagtag gataggcaat gaaatgtgca tatagaaaaa atataacgaa tttttttatt
     8461 tgtttttctg aatcctattg actacaattt ataggattaa tgtatagtat ttttaaattt
     8521 atttaaggaa attttaaatg agtgacaatc acaatccaca aggctattgg acctttttgg
     8581 ttatcttatc actaaatata ctcttttttg tatatatctc ttttattcat ccaggtgtag
     8641 aacctgttga taaaattaaa gcttccaatt cagtaaaaca ggaagagaaa aagtaaaatt
     8701 ctctttctat ctatcttaag ttatttttga tctatggtgg atacaagact cgaatatata
     8761 tgtctagatt taatgggaaa cagtttatga ttctgaaatg tttaaaaacc tatttagtat
     8821 ttagtttttt atttcatttt ttctcaatga atttgttttc ctatgatcct agctacacac
     8881 aaaataataa tgaaatgcct tctgaaatcg tagatatgaa ggtagttgaa aaattaggaa
     8941 aaaatataaa cttaaattta aaatttaaag atgaaaatgc aaatgaagta accctacaga
     9001 attatttcaa atcagataaa ccagtctttt taactatcat ctattataag tgtccaacac
     9061 tctgcaattt tcatttaaat ggaatatcta aagtttttaa atcactcgat tggtctgttg
     9121 gaaaagaatt cgaatttgtg gctgtgagta tggatccaaa agaaactcca gaacttgctg
     9181 caaagaaaaa ggatgcctat ttaactgatt ataaaaaaag tggaaccagt agaaccaacg
     9241 acggctggca ttttctgaca ggggaagaac ctcaaataaa agaattagcg gattctttag
     9301 gttttaaata caaatggaat gccccaatta aacaatggat tcatccagct gtggcttata
     9361 ttattacacc ggaaggtaaa atatcccgct atttgcatgg aatcgaattt ggagagaggg
     9421 atttaaaact ttctctagtc gatgcctcca atggcaaaat tggtaacttc atagataaat
     9481 ttgccttatt ttgttttcaa tttgacccga ataaaaacaa atatactctg tatgcttaca
     9541 atataatgag aatcggtggg gcgattacag tggtgatttt gggtgcgttc ttatttgctt
     9601 tttggcgact acaaatgaaa aatacaaata caaagggaga attctaaatg tcttggttga
     9661 atattcatct ggcaaatact tttatgccag tagagggaac agatttagcg ggtggagtag
     9721 ataatcttta tatctttttg ctcatttcta gtctgattgc ttttgtaatt ttaatcggtg
     9781 gaatgactgg ttttattatc aaatacaaac gtaaaagtgc gaatgataaa actgcttata
     9841 ttacacacaa caatgtgcta gagtttctat ggagttttat tccattcgtt atcatgatgg
     9901 tcattttcta ttggggtaaa attctttatg atgaatatag aactgttcca gaagatgcag
     9961 aagagatcca tgtaacagga agacaatggg tttggagatt tgcttataaa aacggatatg
    10021 catttagcac aaaaccagta agtgatgcaa tgttaaatgt accagtaaat aaaccaatca
    10081 aacttgttat gacatcgggc gatgtaatcc acagctttta tattcctgga tttagaaata
    10141 agcaagacgt agttccagga agatatactt acatttggtt caaagcgaca aaagtgggtc
    10201 agtttcaagt attttgtact gaatattgtg ggttagagca ttccaatatg ccagctatga
    10261 ttaacgtaat gccgcaagca gaatatgatg catggtttaa agcctatgtt ccagaagcaa
    10321 gtgctggtgg tttggctgac aaaggaaagg aattatttgg cgaaagaggt tgtacagctt
    10381 gccattctat caatggaaca agaattgtag gacctacaat gaaagggctt gccggatcta
    10441 agcgagagtt ttcggatgga acatctgctg ttgcagatga tgcatattta agagaatcta
    10501 tcctaaattc tgctgccaaa attgtaaaag attatccggc tgctatgcca atctttaagg
    10561 gacagatcga agaagaagat gtctcagcaa ttattgatta tatcaagagt ttaaagtagg
    10621 agtgaacatg gcgcacgcga cagctacaga tacaggtcat gattatttaa gtcatcaaaa
    10681 aggtctttgg tcttggttaa caaccataga tcataaacga attggaatca tgtatatgat
    10741 tgtgattctt ttcttttttc tcattggtgg aatatttgcc cttctagtta gaatggaatt
    10801 attctctgca ggtccaacac tcggtattga ttcaggaacc tacaaccgtc taatgacgtt
    10861 tcacggagcc ataatggtat ttatggtgat tgttcccggc attccttcca tatttggaaa
    10921 ttttttaatc ccaattatgt taggagcgaa agatgtggca tttccaaggc taaatttagc
    10981 tagctggtat atctttatgg ctggagcgat tactgcattt gcatctttgt tctttggtgg
    11041 tgcagatact ggttggacat tttatacacc ttatagcatt aggcaaccaa ccggagttat
    11101 ctttgttgtg ctaggtgcat tcacaatggg tatggcatct attctaacag gattaaattt
    11161 tattgttact gttcacaaat taagagctcc tggacaaaca atgtccagaa ttccattatt
    11221 tgtttggtca acatatgcaa ctgcgattat tcaggtttta gcaactcctg ttcttgggat
    11281 aactttgtta ttattagtgg ctgagaaaac tttaggggtt ggtatttttg atccaaccat
    11341 tggaggagat ccagtattat ttcaacattt cttctggttt tactctcatc cagctgttta
    11401 tatcatggta ttgccagcaa tgggtatcat cagtgaatta gttgctactt tttctaaaaa
    11461 gactatcttt ggatatactg caatagctta ttctagttta gcgattggtg cggtgagctt
    11521 tatcgtatgg ggacaccata tgtttgtaag tagccaatct gaatttgcgg gcatcatgtt
    11581 ctccgttatt acaatgcttg ttggggttcc aaccgcaatt aaattgttta attggattgc
    11641 aaccatgtat atgggatcta tcaagttgga tgcaccgatg ctatttgcga tcggatttat
    11701 gtttttattt acgatcggtg gattgacagg tgtatggtta gcaactctcg gtatggatgt
    11761 gcatttccat gatacttatt tcgtagttgc tcactttcac tatgtaatgg tgggtggaac
    11821 tcttatggca ttcactggtg gaattttcta ctggtttcca aaaatgtttg gaaaagtttg
    11881 caatgatagt atagctcgaa tttcttgggt gttcattttt accggcttca atgtaacatt
    11941 ctttccgcaa tttatcttag gtgcgatggg aatgccaaga agatattttg actatgtgcc
    12001 acaatatgaa tctttaaata gaatttctac gataggttct tggttgattg gtttaggatt
    12061 tttggttgcg ttatttgcta ttatacaagg tcttctcaaa ggggaaaaag cttccaataa
    12121 cccttggggt ggaacaacat tagaatggca agctacctcg ccaccacatc atgaaaattt
    12181 tgaaaaaact ccaattgtaa gtgtaggtcc gtatgagtac cgttagttca gaatcccatt
    12241 ttcatcatgc tcatcacttt gatagtgctg agcaccaata tgcttcagct aaacaaggtg
    12301 tttggctgtt tatggttacc gaaattttaa tgttcggtgg gttgtttgtt gcctatttga
    12361 tctataagtc attgtatgca ccagtgtttc atgtaggaag ctcttattta gatgtaaaaa
    12421 tggggactat taacacattt ttactgatca ctagctcttt tacaatggct ttgggcattt
    12481 acttcaatca gaaaaatgat agaaaaaagg cagttttatt tttagctctc acgattctat
    12541 gtgcccttgg gtttatggtc gtaaaattct ttgaatatag ccataaaatt catttaggtt
    12601 tagtgccagg taaatacttt aacaatcctg agttcgtaca aaaagtagca gattttgcta
    12661 acaacatgca agcgcaatct tctgctcatc aagatatgga tatagcattt ctaaagaaaa
    12721 ttgctccttt gtattttggt ttttattttg taatgactgg acttcatggt ttccacgttt
    12781 tagttggtgc ttgtttaatt ctttgggtgc ttataaaaac agcaaaggga aattttagtt
    12841 cagaatatta tactcctgta gaaggtgtag gattattttg gcacattgtc gatttaattt
    12901 ggatatattt atttccatta ttatacttgg ttggataagg agataccatg gaagaatttg
    12961 ttaattacgg actttatttt attgcctgtg tttgcgttct agttccactt tttggattag
    13021 gtttagctcc agggattatt gtaaatttag aagttgctgc tattcttgga acactctatg
    13081 cccagatagt agagaaggga ttaattaaaa cattgattaa agataagggc gatactgctt
    13141 taatcaaacc aatggttcca actttacaga acatccagaa tactattgat tcaattgaat
    13201 caggtgatat gggtaaaaaa gaagagaaac acgatcatga agatcatggt caccatataa
    13261 ttccaattcc tgtttatgca ggtgtattgg gtatccttgt attggggaca attataactg
    13321 taggagttgc tcaaattgat tttggagctt ggaatacagt gatagcaatg tttgttgcaa
    13381 cgattaaggc aagctttgta ttattatact tcatgcattt aaagtatgac aatgcaatga
    13441 accgtgtaat ttttggttct gggttcttct ttttattaat cttgtttgga tttagtatcg
    13501 ttgatatcct aactagaaca aaattattac ttggattcac aaagttttaa tgagtttagc
    13561 tacctgtaaa tgcaatacag atcggaactc tcctaacgca gaagaaatta gaaaatattc
    13621 aaaaatgtct atgtttcttc tgctatttgg agtatccgca gttcctgtat ctctcgaata
    13681 taaatgtaaa aaatgtggag agatattcga ccgtttatcg gaagaggaat taaagaatta
    13741 taaatactaa gtatttataa attcctctca ttgattcaaa ttatatttca ttattctaaa
    13801 aaaatcacat aacaatcaaa aatattttta aaagatatat cctagtttat taagatgcat
    13861 acttttgaag tttcaaataa aagaatagtt ggctatttat caaatgcatt ctaaaaatta
    13921 gtagtgtgtt ttttagaaat ttatttcagc ttttcctgac tgctatttgt atgattcatt
    13981 caattctatt tataatcgtc aatgcggatc cttttggtag ataagacaat gctaatacaa
    14041 caatctggaa ctgggaattc ataggattca aaagaattta taagaagtaa aaactctggt
    14101 actctaattg cagtaatatt gcgatagacg ggattttatc acagtagaaa ctatctataa
    14161 tggcttattt tggtgaaact agttagataa ataaactatt tgatcaaaaa ctgtcaataa
    14221 aaacaccaaa agagtgtatt tatgattaga gtgagtaatc gaaatttaga atcaaacaga
    14281 tcagaattta aaatcacggc tggttttttt ataaaaatac cttagaaaaa cgttttaaac
    14341 aattgattgc agtaacttag atacaatgtt tggtaaaaaa acaaatttat cttttcaggc
    14401 ttatctagga tatggaaata ataacatttt gaaatgctct atttgaaaaa ttgaatgaat
    14461 tgaatttgca aaacaacaaa ggatagtgta tgcacaataa cgaacaaaat ttatcaccag
    14521 attctcattt gccaaaagca cagggtttat atgatccatc atatgaaaaa gatgcctgtg
    14581 gagtaggctt tgttgcacat ataaaaggga aaagctcacg cgatatcatt gataagggta
    14641 tccgattgat gtgtaatctt gagcacagag gtgcagaagg tgcagaaccg aaaacagggg
    14701 acggtgctgg aattaagatt atgattccgg ataaattttt tcgtaaggtt ttaccatttt
    14761 cacttccacc ttttggggag tatgcggttg gtatgctttt ccttccacaa gatcctttaa
    14821 ttcgagaagg cgttgagaca attatagaaa aagttatcat tgatgaggga gagcattgct
    14881 tagggtttag agatgttccg gtaaacccag atgttgtggg aactgttgct cgtaaaacaa
    14941 ttcctgtttt tagacaagta ttcattggta agagtcagaa tacagaagca ggggatgcct
    15001 ttgaaagaaa actctttctc attagacgta ttattgatag aagatttaga actgaatata
    15061 aattagatcg cagccaatac tatgttccta gtttttcttc tagaactatc gttttcaaag
    15121 gaatgcttct tggaaatcaa gttaagccat tttatttaga tttaagttct cctgatatgg
    15181 aaagttcatt ctgcttaacc cacaccaggt tttctacgaa tacatttcct acatgggatt
    15241 tagcgcatcc ttatcgtcta attgcgcata acggtgagat caatacattg caaggaaata
    15301 ttaattggat ggctgcacgc cagattgtta tggaatctcc ttattatggt gcagaactaa
    15361 agagaatgct tccaattatt atggaaggac agagtgatac agcgactttt gatacagtat
    15421 tagaattgct ttgtatgggg ggaagaagtt tacctcatgc agtaatgatg atgatacctg
    15481 aagcgtggtc aaaaaatact gcaatggatc cagacagaag agcattttat gaataccatg
    15541 caaccttaat ggagccatgg gacggtcctg cggctatcgc tttctgtgat ggtaaagtaa
    15601 tcggtgccac tctagatcga aatgggttac gccctgcacg ttataaaatt acaaaagatg
    15661 acattgtaat tcttagctct gaagcgggtg ttttaaatac agagcctgat atggtcttga
    15721 aacaaggaag actcagacca ggaagaatgt tattgataga tttagaaaaa ggtcaaattt
    15781 tagatgatga ggcaattaaa agagagatta gtactcagaa accatataga aaatgggtag
    15841 aagagaatat ggttcgactc aatcaattac cggatgcagc cggaacaaag aaagaagaaa
    15901 atcataatac tatcatagaa caaatgagag cctttggtta taattcggaa gacttgttaa
    15961 ctactatgaa gccaatggcg cttaacggtg aagagccaac aggatctatg ggttcagatg
    16021 taagccttcc agtcttatct gacaaaccgc agccactttt ccgatatttt aaacaaaact
    16081 ttgctcaagt gacaaatcca ccaattgatc caatcagaga agagttagtg atggaactaa
    16141 cgacatatat tggaccggaa ggaaatcttt tagatgagac tccagagcat tgtcatcgtc
    16201 tagaattgga acacccagta ttaacaaatg atgaattgga gaaaattaaa cgaatcaatc
    16261 aaggttctct aaaagcaatc accttttcta ttttgtttga tccaaacaaa aagcacgata
    16321 tgcgacattc ccttaaccaa gtatgtcaga aagcagctga ttctgttcgt gctggctata
    16381 atttaataat catttctgat agaggtgtgt caaaggacaa agcagcaatt ccaagtttac
    16441 ttgctgtttc tgggattcac cattatctaa ttcgcgctgg tcttagaaca aaaactggtt
    16501 tgatcttaga atcaggtgaa cctagagaag tgtctcattt tgcactcttg tgtggatatg
    16561 gagctaatgc tgtaaatcca tatctagcgt ttgacgcttt aaatgattca attcgagaag
    16621 gagttttttc agatatcccg gactataaaa cagcgcaaaa gaactttata aagtccattg
    16681 gtaaagggtt gtttaaggtt tttagtaaaa tgggtatttc tacattgcag tcttattgtg
    16741 gcgctcagat ttttgaggca ataggattag acttagaact cgttagcact tactttactg
    16801 gaactgcaac acgtataggt ggtttgagtc ttgaaatgct agaagaagaa acagttcgaa
    16861 gacataaaga tgcatatgat ccgactttct ttcctggggg actagaacct ggtggagttc
    16921 attattatcg taaaacgggt gatccgcatt tattcaatgc gcaaaccatt cataaattgc
    16981 aacaggctac aataaagggt gattataaaa tttataaaga gttttcaaaa ttaatcgatg
    17041 accagaaaga aaagcagata acactgcgta gcctctttca aattgatgag aaaaagtgta
    17101 agccaatccc aatcgaagag gttgaatctg tttctgagat agtgagaaga tttcaaacag
    17161 gagcaatgag ttttggttct atctcgcatg aggcgcatag cactctagcc atcgcaatga
    17221 atcgaatcgg agcaaaatcc aatacaggag agggagggga agatgaagaa aggtttaaaa
    17281 ctcttccgaa tggagatagt atgcgatctg ccattaaaca agttgcttct gctcgttttg
    17341 gagtgactac aaattattta gtaaatgcag atgatttaca aataaaaatg gcacaagggg
    17401 caaaacctgg agagggtgga cagttacctg gtcataaggt tgacaaaaat attgctagac
    17461 ttcgttttag tactcctgga gtaacactca tttctcctcc tcctcatcat gatatttatt
    17521 ctattgagga cttaaaacaa ttaatctttg atttaaagaa cgtcaatcca agatcaagag
    17581 taagcgttaa gctagtatct gaagttggtg taggaacagt tgcggccggt gtagctaagg
    17641 ctcatgcgga tcatatttta atcagcggtc atgatggagg aacaggagca agtccacttt
    17701 catctattca ttatgcagga actccatggg aaattggttt agctgagact catcaaacgt
    17761 tagtcatcaa tggtcttaga gatcgagtat atctggctgt tgatggtaag atcatgactg
    17821 gaaaagatgt agtgattgga gccttattag gagctgagga gtttggattt tcaacttcag
    17881 cactcattac gatcggttgt gtgatgatgc gtaaatgtca tttaaatact tgtcccgttg
    17941 gtgttgcaac acaagatgca catcttagaa ctaaatttac agggaagcca gaatatgtta
    18001 ttaacttcat gcattttata gcagaagaag ttagagagtt aatggcacaa atgggattta
    18061 gaacctttac tgagatgatc ggtcaagtag aaatgatacg ttttgataga ccaaagaatc
    18121 attggaaggc gagaggactt gatttttcaa aggtgttagc gaagccaaaa ccagctcatt
    18181 tcccgactga aatgtataga acgaaagagc agaaccacgg actagaacga caaatggata
    18241 atgaaattat tcgtaggtct agagcggcta tcgattataa acaaaaagta aaatttgata
    18301 tgaaaatcat caatctgaat ctttctgtgg gaacaatgct tgccggagaa gttgcaagac
    18361 gttatgggga attaggctta ccagaggata caattgatat tacatttaga ggaaatgcag
    18421 gacagagttt tggggcattt attactaacg gtatcacact cagactcatt ggagactcca
    18481 atgattatgt aggaaaaggt ttatctggtg gtaaaatcat agtagctgca ccacaagagg
    18541 cagcttatga cccaactgaa aatatcatcg ttgggaatac ttgtttttat ggagcaacca
    18601 aaggaactgc ctttatcaat ggttttggag gagagagatt tgccgtaaga aactcaggtg
    18661 ctaaaattgt agtagaggga attggagatc atggatgtga atacatgacc ggtggttctg
    18721 tgattgttct tggtaaaaca ggaagaaatt tcggggctgg aatgtctggg ggaactgcct
    18781 atgtttggga ccctgatgga tcttttccaa aaaatgttaa cttgcaaatg gtagaattag
    18841 aaaaactatc tgatccggaa gaaattgcac aggtattcaa attgattact cttcagaaag
    18901 aatacacgaa ttcaaaagtt gcaaccaata tacttgcgaa ttggaaaaca gaggtagaga
    18961 aatttgtgaa agttatgcca acagattata aaaaagctct cttgaaatta aaagaagagg
    19021 ctgcaaacaa tatcaacacc aaggaggcaa gtcatgggta agccaacagg atttgtagaa
    19081 tacaaaagag aaggattaaa atacgaatcg aaagaaactc gtattcaaaa ttataaagaa
    19141 tttgaaaagc cttttgaaga aggtgtagct aaggtccaag cagctcgttg catggattgt
    19201 ggaattccat tctgtcatag tgaaactggg tgtcctgttg ataatttaat tcctgagttt
    19261 aatgactttg tttataaagg aaagtggaag gaagcagcag aaaatctgca tagcacaaat
    19321 aattttccag aatttactgg tcgtctatgt cctgctccct gtgaaggagc ttgcactgtt
    19381 ggtttaattg atcagcctgt ttcgattaag gcaatcgaaa gaacgatagt ggatagagct
    19441 tttgaggaag gttgggttgg cccgaagcct gcaagtagta agtcggggaa aaaagttgca
    19501 atcgtaggat caggtcctgc aggacttgct gctgctcaac aacttgctag agccggacat
    19561 gatgtagttg ttttcgaaaa agcagatcga attggtggac ttctcagata tggtattcct
    19621 gattttaaaa tggagaaatg gcatattgat agaagagtga agcaaatgga agcagaaggc
    19681 gttgtattta agactaacgt tcacgtggga aaagatatac aagctaaaga gcttctaagt
    19741 ggctatgata gtgtaattct tgcaatgggt gcagaaaaac caagagactt aaatgttcct
    19801 ggtagagaat taaaaggtgt tcactatgct atggattttt taacattaaa caacaagaaa
    19861 gttgcgggtg attcggttga ttacatttca gcaaaagata aacatgtgat cgtaatcggt
    19921 ggaggggata caggttcaga ctgtgttgga acttccaatc gacatggagc aaaatcaatt
    19981 actcaaatag aattgtttcc cgagccacca aaagatagag atccttctac tccttggcct
    20041 ctttatccta aaatgctaag aacctcttcc agtcaagaag agggtcaggt tattcgaaaa
    20101 tggtcgatca atacattagg ttttagagga aatgaaaaag gggaagtgac tgcaatttta
    20161 ggcaatcaag ttcgttttga aaatggaaaa tttgaaccta ttccaggaac tgaatttgaa
    20221 tggccagcgg atctagtttt acttgcaatg ggatttgtgt cacctgtaaa agagggactc
    20281 atttcacaat taatggagca gggtttagaa ttagataaat caggaaatgt aaaagcgagt
    20341 tttggattaa aaaatggcgc ttttgctact tctattccta aagtatttgc ttgtggagac
    20401 gttagacgtg gtcagtctct aattgtttgg gcaatttcgg aaggaagaaa gtgtgctgat
    20461 caagtaaacc attttctttc aacaaaagta tgatcaaatg actctgctag aatcagaagt
    20521 cagataaaaa aattattaag gggtttcaaa tcggaagccc cttttttata taaaaattaa
    20581 attcaccact gatagagaaa ttcccttcta tactatctaa gatttgtaat acaagattta
    20641 taaaaacctt tattttttga aagaattaac agcaccgaaa ttctgtgaaa acgatcgaac
    20701 tatttattcg aaaacaaatc ttattttgat aggtagttta aaccaaaaca atttagcaaa
    20761 gctttacaat gaatcagaat tcaaaattca gactaaagag ataatatctt cctggttggg
    20821 ctaaaataga attacgatag ccgttagatc gttcatagta attatttccg gcatcagctg
    20881 cttcttgtcc cggatgcaaa aaagaatggt ttagtaaatt aaaaaccttt aacacaaatg
    20941 ttgtatattt caaattgaaa gaaaaagccg cattgaataa gaaataatta tttagaatga
    21001 tacctttgtc tctctcaaaa ggcatacctt tatttcttaa ttcattactt agataaagtt
    21061 gtcttggtcc aacataattg actctaagat tgatatttaa aatatgttga atcggtaaat
    21121 ttatacctgc acttatttta tgaggagaaa tgtctcctaa cgtatgataa gactttttcc
    21181 ttggtaaagg aggggcagta ggattagctg cataaaaatc attctcatat tttccaagaa
    21241 ctgtagtccc cgatatccaa tcaacaggat tggtattctg cggagtccat gcagttctag
    21301 gagcattgaa attataatat gattcgctta tcgcttcggt aaatgtataa taaaaataca
    21361 aatctaattt gttagaattt gggattggat tgttgagttg aattttattt ctaaattcaa
    21421 aaccataaat ctttctagag ccttcatttc tagcagtttc cttaataaca ttttcatacc
    21481 tagaatagta aaaagacaat tcagataaaa cactagaaaa ttgcttcata ataattaact
    21541 ctgttgttct ttccttttct ggttttaatt tattgtttgc ctgtctacca gaccatccgc
    21601 cataaaggag aataaagggt ggctcttgaa acgcttcgcc atataaaagt ttaattgttc
    21661 ccgtgctgct gtatttatag attgctgaag ctctcggatt aatggattgg ccgtagatag
    21721 aattgtaatc atatctaatc cctggactga atctaaattt tccaatctca tatgtcgtaa
    21781 ttgcaaatcc acctagatca tacacctgaa ctatattgtc tttgggcata tttgtttttc
    21841 catctgcata tttataatag atgggttggt cactgtattg aactgcataa ccatttggga
    21901 aatagggcgt tagatctaaa gcttgctttc cctcataacg gttccaagaa tcataggacc
    21961 caggccaata tccaggaata tcatattgct tggtcatatc tcttttttct acttttagac
    22021 cggttgttaa taggattgaa tttgaaatct tataatcaat attatggttt aaaagaaaac
    22081 ttttgttttg attgccccaa taggtctggc ttagataaga atacatttct ttaccttttt
    22141 ttgtgtctgg ttccgcttct gcccaaggac ctctaaatgc tgattctctg aaggtaaata
    22201 aagtatttgt ggtaaggtta ctcgaaacgt tagacgtata ttcgatatat acttgtcgac
    22261 tcataacttc ccagaaacta ttattttgcg ctctatcgcc gggatatttt actccatagc
    22321 cttcttttct gctccaagct atagttccaa tctttaagcc tccgtttgaa acacttgctt
    22381 ctaaaccata attcttcgta ggatcatgat accttgataa attcactcca tcattttggt
    22441 agtatcgaac tccaccccaa actgttgggt tgccatacca ataattagaa ttaaatcctg
    22501 atcgattgga taagtccggc tcattgctag aaaataattt tccagaagcg gcaaatgccc
    22561 attcgccaat acgaattgtt gagctagcat ctacaccttt ggtattattt tcaccccctt
    22621 gtaatgaaat tttagaatag gaagattttt caatctctga agatttcatc tttctaggct
    22681 cagttccatc atgagtaatg atatttataa ttccttgaaa agcatttgca ccgtaaacag
    22741 cagaagctgg tccgtataaa acttctattc ttttaatatt ggtcatagga atttgtctag
    22801 aaagctctgc ttcttgtgta tacatatcgt tttgaatttt attatctatc ataaaaagtg
    22861 ttctagacat aaaaggagtt cggtatcctc tctgataacc cataatataa ggaatcccat
    22921 ttgaaaaaga caaatcaaat ccaggtaagt catataaaat ttcatcgata ctttgatatc
    22981 cgcgatttct aatatcttct tcagttataa ttaccatact ggctggagca tttttaagtt
    23041 tttcttttgt tctagaggcg gtaactacaa tattctcttc tattttaaaa aaattctctt
    23101 caatatgttc atcttttgat tgtttaggtt tctctatctg attgtttgtg ttttcagatt
    23161 taggatctgg gtccactgct ttttcttgtg ctaggattcc tgataaagag atgaataaaa
    23221 taaaaataaa ttgtatcata aaaagcatcc aaagtaattc gattcagaga catataaata
    23281 taaatgtgag gctaatggca acagaaaaat agtagatgct cttacaactt ctactgatta
    23341 taaaatttta ttaaaggaga tttagcgagc ctatcggtta tttgaaagga aattcaaacc
    23401 ctctattcaa tgcttcatta gaataacata atatagtaat atcttctatt caaagtaatc
    23461 atttcttatt ttttccaaaa tgtcagggat taaaattttt aactaacagc attcatctaa
    23521 catgtattag ctttttaaat tcaaaattta gataaattcg ttttacttac ttggaaataa
    23581 atacaaacaa gtaattcaat ctgtttctat ttggaatgaa atatttttag aaaagatgaa
    23641 tgtattatta ttattattat tattttataa aaatcaaaaa tcatgaatcg aattgttttc
    23701 tatccaatgg aaaaagtatt ttatagtttt tcatttttaa ccggattttc taacaatgta
    23761 atttttctaa attgaattat ctaaacttat aaataaaata agttggttta caggatctat
    23821 ctacaaacta atttgcaaat catatatgaa taaaccaata actggaataa tagcagccca
    23881 aagaacagcc gaaggagagg ggtttatagt aaatagaccc tttcctacta aatttctaaa
    23941 tttttatgat ccatttttac tcttagatga aatgggtccg actatctatg aaaaaggaga
    24001 ggcaaaggga gccccagatc atcctcatag aggatttgaa accgttacgt atatgattga
    24061 aggggaaggg gaacatgaag actctgccgg aaatagaggg atcattgaag caggcggtgt
    24121 gcaatggatg actgcaggaa aaggagttat tcatagcgaa atgccatcta ggaaaatcca
    24181 agaagaaggt gggaaagtgc atggatttca attatgggtt aatttaccgt ctatcgctaa
    24241 aatgattcca ccaaaatacc aggaaaagaa atcctctgag attccaatct atgagggaga
    24301 agggctctgg gttaagataa ttgcaggaga attatttgga gtaaaagcag aaattcatac
    24361 acatactcca attctttatt ggcatgttag gctttctaaa aataaaaaaa tcgaattacc
    24421 tgttccgatt gatttcaatg tatttttata tttaatagag ggaaaaggta tatttgaagc
    24481 aaatggacaa acgttatccg gaaaacaaat gattcaattc tctaattcta aaggagagtc
    24541 aatttattta gaaaatccat caggtgagaa tcttgatata ctagttctag gtggaaagcc
    24601 tctcaatgaa ccagtggcta ggtatggacc gtttgttatg aatacacaaa aagaaattga
    24661 agaagctttt tcagattata gaaatgggaa tatgggaagt attgatagac cttgagttta
    24721 aaatacaaac ctaaaaccga attcaatata cgaattttga tttgtaatat ttaaatttgt
    24781 aggatttgaa tagacttttg tataaagatt gagggtcgct tcttgttttg tgaggctaat
    24841 tgacgttttc catagaagag catctaatat gtttaaagta taaatagaag caaatagaat
    24901 cgatgcggta tatactttat ttgcggaatc ttctcctctt ttaaaatatt cattgctatt
    24961 aaaataatta tagagtaaaa cagaatcctg cttatcaatc atttgatata aaataccttg
    25021 attaatagta ttattgtatt tttttttatc tgaatggaat gtctgatagc tatatgagta
    25081 attcgctcca gcgataataa aacataagtt aaagaatatg gagcttggaa aatgttcttt
    25141 gctgtattgt ccccaaccag ggattaacat cgatttgccg gcttccttcc agtatgggta
    25201 tggattttct ctagaagaat ttggtagact tttggtttcc cctaattgat agaattgatt
    25261 caaagttaga attttttttc taggattttc aagaattaga tcataacttc cttctttcat
    25321 attttcattg tctattgtga tttcaattgt tcctttttta aaaactttat ttttcagctg
    25381 aacagttgaa ttttcatttt ttaaaaagac tttagtatct ttgtatagat tttttccttc
    25441 aattaatata gttttttctt tttctgattt ggaagaaata aagaattgct cgcttgttac
    25501 gataggtgga agggatctta ctactatgaa tggaacccaa tctgtataag aagttacttt
    25561 tccaaacttg ttgtaggttc caactctatg cgaatatctg cctggttcta aatctacttt
    25621 gtaataggtt tccttggttt caatttcata tgtgatttca tctacctcat ttttaatttg
    25681 taatatatac tcagttgaac cttcacttgc actccaagta attgttgggt tatctatatc
    25741 tagaatatcc tctgagagaa tagatagggt aaatagaaat agcattaaga ctttaattct
    25801 attccacata gaatttcttc ggagttctaa tctctggaac tttaggtttt tcagataaat
    25861 atatcttaaa aggtataact actttattgg atttatcttt agataaatcc tgtagagtcc
    25921 aaataaaatt tccttcatct aatttactca aatcctttat ttgataagaa gaattctttg
    25981 ttttagattt tgctattaat gttttctcac catttttatt gttatagagt tctacttcat
    26041 atctggtatc ttttttagtt ttttcccatc tcagcgtgag gctatctaaa ggagacatat
    26101 cgactctttc gtttctagta gggaaaatag gttttaaaat ttcaacttct tctatactaa
    26161 aagataaaat tgcacttttg gaaagaattg aaccgtcatc cgaaaaagct gtgactttcc
    26221 aaaagaaagt tccttcggtg gaaatcgaaa atgaatttga aagctcttgc gttttgattg
    26281 aatgtatttt ttcttgaaag tctttacttg cagaaacttc taatagatac tctgcttgaa
    26341 ctggtagttt tttccatcta aaaacaacag gattgtcttt catatcaaat tcagatcctg
    26401 atttgggaga gattaattca atgggttgat tttcaataat tgaaaatttt cttatttcag
    26461 atggtggtgt gtttttgcca tcttggctaa tgcctattac ccgccagaaa tatgttccaa
    26521 gctctaattt tttatttagt ctgatctggt ttgcggtgac tgtttcattt atagtaaggg
    26581 ctgaaaaatt attgttttta gaaacttgaa aattatacct attaatttct gctaaatctt
    26641 ttgccagaag tataatttca gatttatctt gctgaataga agtgattgat tgtccatctg
    26701 aagggctaat taattgaggg ctttctaaat catttttttt ctcgatgcta aatgaaattg
    26761 ttttagatgg gtcaaccttg aattcttctc taattggatc aatggaaatt cgagcatagt
    26821 atttaccagg ttctaagtta tctactccga gggacgttag attggtagta acggatttaa
    26881 ttatttcgga gaaatccgat tttctactaa tatctaattt ataatttctt gctgtttcta
    26941 atttattcca agaaaaagga atgatagggg ataccgtagt aaagttgaat gtctggtttt
    27001 tagtaggaga gagtagaata ggggatgtaa ctgtaaagat tgttattttt cgaaatgcac
    27061 tgtattccct aatagagcgt ttttttattt tatctttttc tgaactgatt ctccaataat
    27121 acgttccttg ctgtaagttt aaagcgattg aattattttc tgcgttaatg gattgataga
    27181 tattttggaa cctagagtcg taagccaatt caatttttaa attctttgca tttttagaat
    27241 tccaactgaa tgtaacggat ggattttctt ttttgtcagt aagtattgtt ccgtctgcag
    27301 ggctttgcaa tgataagtta acttctgaaa tttcaatttc tgccttgttt agttttgccg
    27361 attcattttc ttttatttct ttttcttgat taccggtctt taattttgca gttccctgat
    27421 ttacctgaaa agttaattct tctttacttt ttttttcgag tgttagatca gagtttttaa
    27481 cttctagtgt attgtttcct gattttattt taagagttgt atcagaatat ccatctactc
    27541 ttttagaaag ggacatggat ccgtaagcaa agttcagatt tatatctcct tcattcatat
    27601 cgatatagat catactgttt tcattgatat taattttagt tttatctttt aaggtcaata
    27661 ctgcatctga aaaactacca gatcgaatcg tatctttatt tcgaatctga agccctgact
    27721 caatgttttc ccagattaca tcggtgtcat attttctctc aattgtctta tgcttaaacg
    27781 ttagtgtgcc taccacttca ttgtcatttc ctgttgcacc cgaatgaata ttcttgtaga
    27841 gtaatacaga ggcaatcagt atgattaaaa gtaatagtaa aataactaaa taagatttat
    27901 ctttttttag atccatagtc tatttatgag ttcttgtata aactccatcc caatttgagt
    27961 caggtggatt cgttttatag aattcacatc tctcaataag ttgtctgcat gctttatctt
    28021 tttttccctt tgctttttcg gattgctgta gaaactgaat ggatttgagc cattcttttc
    28081 ttagataagc tttaaatcct tcttcgtata aaccggtggc ttcttttata tttgaagata
    28141 catctgtatg attagaaatt aattcataga gttttactgg ttcatttttt ccttttactc
    28201 gaaccaagtc tagttctctt gtaaacattt ccttctctat ttccgttttt acagattcag
    28261 aaattaaaat attaactcca taatcttttg cagccgcttc taaacgagca gccaagttga
    28321 ccgtatcgcc catcatcgta taagaaccca tagcatcggt tcccatgaat ccaacttttg
    28381 caagtcctgt atttaggcca atccgaatgt ccatctcttg tgcatcttta gagtataagt
    28441 tgtttttagt ccaatattct cttaggtctt ttagtttatg aatcatttct agagaagcac
    28501 gagctgcctt tatataatgc tcatttacag ggacaggtgc accgaatatt ccgactatcg
    28561 catctcctat gtatttatct agaacacctt cattgttttt taatataatt gtcatagcag
    28621 aaaggtattc attaagtaat cctgcaagtt gaacggaatt taattgctca ctgattgttg
    28681 agaagcctgc cacatcggag aagaaggctg taatcatttt ctcatcccct cgtttaaggg
    28741 ctgctaagtc tcgcataccc tcagatacca caataggatc gatcatacta ccaagcatgc
    28801 ttttaacttt ttctctttct tctagtcctt tagccatact ttgaaaataa tcagttagaa
    28861 ctccaacttc gtcattggtt gttcttttga tctctaattg aaaaatacct tttgaaattt
    28921 taattgtttc atcgagtagt tttaaaagag gatttgagat agattttgca aatataaata
    28981 cgatgataag ggatacacag atcgaaatga tcataatcaa gatatttctt tgttgaattt
    29041 ggtaaactgc tttataggct aagtcttcct caaccacaga aataatacct gcattagcaa
    29101 atcctaattt ttgaaaggct cctaaatatt tttttccatc tgtgtcctga aacggtttta
    29161 attcgttctt tgcttgactc gttagcattt gacgcacaat aggggttgtc ataaagtttt
    29221 ttccagaaga aataatcttt ggatctgggt gagctaaaac cattccatct tcatttacca
    29281 tgtatgtagt attgattcca gattttttaa atgcatccaa gattttttct aatcttaata
    29341 gtataacaag tatgtattca gatttttctc cttcttccaa aggaatacta atagagattg
    29401 ttgtttcttg tgttccaggg ctggtattta gaagtgtagg cactccttca aaagatttgg
    29461 aaaaatattt tagattttga tgaatcggcg aaatcacatc ttcttcggta agggaaactt
    29521 tctttaagta ttcctggtta aaaatactat ctactgtttc cattttccca ttttcctttt
    29581 tatatacacc tagaaataga aagctattgt catttttgaa gtattgttca atgaaaaatc
    29641 ttttttgaga attgctttta aattcttgtt tgagtgtaag ggctagaatt tttcccttat
    29701 tgatttctgc gacaaggtca gttactactt tggaattaat aattcgcaca atatctaagt
    29761 tgttctgatc aattcttttc tgattatcat ccttaaagaa ataagtggcg agtgcaatca
    29821 taactgaaac ggtagttgca atcacgattg atataaccaa catcattttt aactgaagag
    29881 aatattttgc ttttgatgta ggcttatcgg gtgtagccgt atctgagttt actttttcag
    29941 tagaagtgga aggtgtttct cttaaattct ccattttggc aagaaagcca gttttttgag
    30001 ttttgggttt tggatttttt tcaatgctag aattagaact tagttcatca ccctttgttt
    30061 tctcatctac taattttggc ttttgaactt ctttcgaagt ttcatcagaa tttggttcca
    30121 gattgttttc gattgattta tgaatctctg ttatacttgt gttgatatcg agtataggaa
    30181 tcgcgtccat cgtaaagtca ggagtatact gaattcggtt tttttctggt ttattttctt
    30241 ccataataca ttttataaat tcaaggcggt aaaatagcaa ataattctat atttgtcaat
    30301 aaattttata tgtatcgaat agaaagtttc aactagtatg tcctaaaaaa tgcataccag
    30361 tagatttttt aatcgaattt tcttgaaaag aaggtattct ataattgttg tttagaaaaa
    30421 ataagaccat ttccgagaaa tggaatgaaa tagatatgaa aacaaatctg tttataatag
    30481 gaacaatcat tttactcgct cagtgtagac caaacctaga taaatctgag ataccatcca
    30541 ttagctatct actttataag caaaataaga atacaaatac acttagtttt gcctatccta
    30601 gtcaatccta ttccttttta gtaaatgttc caattgcaac acaaagtcca gttgcatcca
    30661 ctgtctttga ttcttattct gtcagtcctg ctttgccttt aggcttaatt tttgatacaa
    30721 aaacaggtca aatatcggga actcctacaa ctgcaagtcc ttcatcaagt tatactataa
    30781 cggcaacgaa ttcacaaggc tctgcaacat ttactattag catcaccgtt accaattcac
    30841 ctcctacagg acttgctttt accggaagtc cattttcttg gacagaaaat caatctatta
    30901 ctgttactcc tgttgtagtt ggagttgtaa caaattgtat ctctacacct aatttaccta
    30961 cggggctaac aatcagtagc tcttgtgtga tctctggaat tcctaccatt gtgcagggaa
    31021 taactaccta tacaataact gcttccaata tttatggaag catttctgtt gctgtggata
    31081 tagcgatatt agcctctcct attaagagaa tatttgtaac gaatagtgga tatacaccta
    31141 acgtcgcttt taataattta gctacggctg ataatatctg caatactgat tctgcaaaac
    31201 cagcagggtt gggaacctat gcggcgatga ttgcaattcc tctgcttcga gttgct
//
LOCUS       DAOQOS010000063        30628 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_666_length_30628_cov_6.159193, whole genome shotgun sequence.
ACCESSION   DAOQOS010000063 DAOQOS010000000
VERSION     DAOQOS010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 30628)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30628)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30628
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_666_length_30628_cov_6.159193"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            457..2682
                     /locus_tag="PLF66_14430"
     CDS             457..2682
                     /locus_tag="PLF66_14430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HMZ65290.1"
                     /translation="MRSILILLLLFFSQCLTAKKSPFDFRSPNVLLGSLYFSFFGDYS
                     VSGIITGFTTGTLILQNNLANDLTLSPNTIYSFGGLKRGTIYSITIKSNPVGYVCSIE
                     NFSGIISANVTNANITCAPIAITALYPQNGSNWNDYINKGSGDIFSQPDTACNTSNTG
                     GYRTCIHAGEIRSFELPSRSSCTNLSATDNLDALNWICKTNSKGSVTFYSTALKRGKY
                     LSNLMDWNTSSWQNLTITIKDGSTTLAVSNPIKLWSNAIDTTEPATPSTSGTIYLYGT
                     TTFPTNIIINANKIALLINPNGIKLIRSAGTCNSIAGIDITNRSFVWLEGNMDFGNST
                     QNSYGISITNSKFSVFKNIRIQNTGVNCITTGNINNAINLNGSSNDYFEDIYLGQTFG
                     TSPANGNGLLLTASDDNVFNGISISNMATDGIQLNSSKGNTIINATVMSSPAECVLIQ
                     SSSNNNTFLNLTTVNSNNYGVRSVGSGNGLFQNSVFFNHLNHGLSLFTGSSITVQNLI
                     ASNNNSAQVNLNTTSSYFTGIIKTSSTTCINTASSGGVTSTCQPVSPSDFSAPISISP
                     SSNPFVGYSATDSKNTTANLSGNPSGIALASMTDWVRFDHLYKGIGKFNSASFPSTVH
                     LGVCNSANCTIWDFSLKASDTILRNANIPSASSCPTTTVTHSFSTSTVTFLRNSIEIL
                     NDGVGNENGLCEANEDCLLTPNIGSYQGHGNLVSASSLSGCNDITSLGIKLYQYESNG
                     Y"
     gene            2806..3906
                     /locus_tag="PLF66_14435"
     CDS             2806..3906
                     /locus_tag="PLF66_14435"
                     /inference="COORDINATES: protein motif:HMM:NF024813.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HMZ65291.1"
                     /translation="MGIFDKLKGEFIDVIEYTDDSLSGLVHRFERHGNEIKNGAQLTV
                     RESQTAIFVNEGEMGDVFKPGRYELTTKNIPILTTLKSWKYGLNSPFKAEVYFVNTKI
                     YTGLTWGTKNPIMLRDPEFRQVQVRAFGTYSIRVNDPIKLVKEVSGTSSSFDIENVED
                     QLRSLLVSEFTDALAEMSIPVLDVARNFKELGAKIQGFVNEEFGKLGLEVTKFIIENI
                     SLPPNLQKFLDEDTGAAMVRDVNKVNQMKMGDAMLNASQNEGAGGVMGMGMGAGMGMA
                     MGQQMGGMGQQQQTNNPPPIPGAVAYFVAVNGQQQGPFNLDQLKTAISNGQITRDSLV
                     WKNGMANWVKAGDNPETSGLFGSVPPPLPPQQ"
     gene            3925..5037
                     /locus_tag="PLF66_14440"
     CDS             3925..5037
                     /locus_tag="PLF66_14440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65292.1"
                     /translation="MEEVAADKKEFPCSQCGAKLTFKPGTDKIVCGYCGHEQVVSQPK
                     AEATGTTQIVEHDFNDAVKNARSVPASQVVSGGKEIQCSACGANSILTGQSDRCAFCG
                     SPLVAEVKGQEAMFVPESLLPFHIDKAQAKQKYMDWIKGLWFAPNDLSKRATASGMDG
                     VYLPYWTYDADTYSRYTGQRGEYYYTTESYTDSKGNTQTRQVRHTRWYPASGSVSVDF
                     DDVLVCASKSLPQEMIDELEPWDLQELKPYDARYLSGFVTERYKIGLEEGFSVSKEKM
                     DGPIRSKVRQDIGGDEQQITSLNTTYSNVKFKHLLLPLWISSYRYNEKIYRFVINART
                     GEVNGDRPWSWIKITLASIAGLAIAGAIYYFTQNAG"
     gene            5260..6933
                     /locus_tag="PLF66_14445"
     CDS             5260..6933
                     /locus_tag="PLF66_14445"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HMZ65293.1"
                     /translation="MYKSSYIEGKKKPVLLYIDSDPVYLEKIYSILRKEFEKDYEIQT
                     AGSHLIALEIVQTCNRNLIELPIVLCSQQIPVIRASEIIAIIHETNPSTRKIIFTESI
                     SSEDLLTAINKANIFKALPKDIEEKDLVSIIQEGIKSFYSEKDLREKNVKLEKLLFFN
                     NDTNLANWERFQQRLLEYSRKNLSLTVAIIKIENYTYFLEYFGAKIYKKILLELIEII
                     SSTALSGEEMFHTQQDEFLVLSKKPVTEDFIYKFSVLRLLLKSQYIVVENLSFQISLF
                     ISITSGNKNLYSTAKSGLIHRFNGSGPTNASIHINEIEFHKENILWGQKLNTAIQNKL
                     ITPFYQGIQDNKTGEIVKYECLVRMHDNGEIIYPDKFLNLASAMGLMKLITIIVMDKA
                     FSHFQNSNKSISINITQFDLEYKDFAQLVQAKLALYKINPSRITFEILENVSFGLESQ
                     TLKTLHELKEIGCKIAIDDFGVHYSNLARLLEIQPDYLKIDGWFIKNIIQNRNAYLVT
                     KAIIELSHSLNAEVVAEFVTNEEIQNKLVEMGVEFSQGYFIGKPEEKIF"
     gene            complement(7146..7436)
                     /locus_tag="PLF66_14450"
     CDS             complement(7146..7436)
                     /locus_tag="PLF66_14450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65294.1"
                     /translation="MDLNTEMYPIDFSEDELEVLKGTSVPYKTIHKKLTSTKKHKDGF
                     YYIKFTIKEIRELNEYLAAILNRDTANESILSEVIDRLDATLKNTAGYPIID"
     gene            complement(7451..8041)
                     /locus_tag="PLF66_14455"
     CDS             complement(7451..8041)
                     /locus_tag="PLF66_14455"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015679614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HMZ65295.1"
                     /translation="MNLIRERKCWIFDMDGTLTVPVHDFPAIKTELGTPLDIDILHAI
                     SLMSAEDAKRAHVKLQEIEFRLASIGRVAAGTIELLQALSDNGCSLGILTRNSLINTE
                     ETLRASGLNQFFREDFIISRDQATPKPSPDGIHILLDKWNASPNDAVMVGDYIFDLEA
                     GNRAGLKTIYVDPTGEFVFRSHASHCVRQLDEVLEI"
     gene            complement(8038..8439)
                     /locus_tag="PLF66_14460"
     CDS             complement(8038..8439)
                     /locus_tag="PLF66_14460"
                     /inference="COORDINATES: protein motif:HMM:NF025705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4399 domain-containing protein"
                     /protein_id="HMZ65296.1"
                     /translation="MFKYISILLIVFSLVACTKKPTGKVFFLTPQNGEEVKSPVVFKM
                     GVEGMEVAPAGEVQLGKGHHHIIINGESYDSGKTIPFVEKKVFHFGKGQTEASIELEP
                     GEYDVTLQFANGAHISYGPELSSKIRIKVVP"
     gene            complement(8905..9528)
                     /locus_tag="PLF66_14465"
     CDS             complement(8905..9528)
                     /locus_tag="PLF66_14465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004786769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HMZ65297.1"
                     /translation="MSQKRFLNIIFFVSILSFANCGYNRIQELDEEVNANMAEILNQY
                     KRRADLIPGLVKIVEGYASHEKEVLVEITKARSSVGGLQATPEALKDPEAFKNFQDAQ
                     NSLGSALQRLLVVTERYPDLKANEGFRDLQAQLEGTENRIAVARGRYIKAVQEYNTTI
                     RKFPTVLTAKAFGYEPKSSFTVENEGDIKNLPSNITTPPPMDFGTKK"
     gene            complement(9938..11539)
                     /locus_tag="PLF66_14470"
     CDS             complement(9938..11539)
                     /locus_tag="PLF66_14470"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HMZ65298.1"
                     /translation="MNYKLKLFYILFLIINLFAESCNIGKGLANQNFISYLVRLRDGN
                     EAIGNNPAPPNPATSLNVVGITPINNSINYTIGTPITILFNKPIDLSSLTIIGTNGPC
                     GSNVQISTDNFATCLGGTVTLSPDSLAANITLTIPLCVETTYTIQVKVTSAVKDTTGI
                     TLASTYTSSTSFQTQPAFVKVGVSGGTNTVSALAYSCNFLFVGGNFTTLGASSRNNLG
                     VIDLVTGNESSVNMGTNGAVTALLAYNGLIYIGGSFTNAGGQPRNNGAAIDPRTGAAT
                     PWNPNASGQINAIVQNGTTAYVGGAFTTIGGQARNRIAEIDLNTGNATAWNPNANTAT
                     INAIAYDATNVYLGGSFTSITIGGQTFTHLAAVDRTTGNGLAGWCGTATTFPVNALLL
                     TGGVLFVGGAFGGNLCNLVIYNFGGLVPSTAANGGYPMGGGTGAGNAVKAFSVYGNNL
                     YVGGIFSGAGSFFSNVRNYLGSTSLSGVINSWDPNAAGGSNGVTAMVVIGSGIVVAGD
                     FTTINGGTAKNRLAFVDTNTGALRP"
     gene            complement(11710..13203)
                     /locus_tag="PLF66_14475"
     CDS             complement(11710..13203)
                     /locus_tag="PLF66_14475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012387862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008775 - acetate CoA-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate CoA transferase"
                     /protein_id="HMZ65299.1"
                     /translation="MNPYIQSKVMTAEDVANRMPIQGTIATSGFTPAGYPKVIPKAYA
                     ARIEKEKQAGKNPWFNLYTGASTGEELDGVLGRTGCMKKRLPFQSTPDVRNRINAGEI
                     DFVDMHLSHVAHYIRDGILEPIDVAIVEAVDVSSDGRIWLTTSSGMSRTYLHHAKEIY
                     IELNSNHPIELKGFHDLTAPEQPPHGRALHISRVDDRIGRPYVVVDPEKIKGIVLTNL
                     EDHSGDFKAPDANSVLIAQHILEFILFEIKKNRIPKEYLPFQSGVGNVANAVLSCIAK
                     DSRFHEIEMWTEVLQDSIFDILEVGKLRCASTSALTFSKAGHQKFLDDITELKYKFII
                     RPQEISNHPALVRSLGIIAMNTALEADIYGNVNSTHVTGTGMMNGIGGSGDFARNAYV
                     SIFMAPSVAKGGAISAFVPMVSHVDHTEHDTQIIVSEQGLADIRGLAPRKRAEVIIEK
                     CVHPSYKDAMREYLRDSFATAKTKHTPHDLKKAFNWHIKLLETGSMK"
     gene            complement(13459..14220)
                     /locus_tag="PLF66_14480"
     CDS             complement(13459..14220)
                     /locus_tag="PLF66_14480"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HMZ65300.1"
                     /translation="MKKIKLGNKELPVLGLGLSGILPRVIQKNNTPFKESIVNALSIG
                     YRFLDTAEIYGFGESEKLTGEALYQSEVPREKITLSTKVWFINLSRKRLQKSCEMSLR
                     RLKTDYIDIYSIHWPNPLISLDETIEVLDRFISSGKIRYVGICNAKTEYINKVNQSLL
                     GKVLTVQFPLNYIDRKEEDKIIFLKERNIFPVAYSPLKSGMIQKNKMLKKLGVEINRS
                     PEEIALQWLKEKEISILIRSLSRKHLIDNFKLLNG"
     gene            complement(14195..15040)
                     /locus_tag="PLF66_14485"
     CDS             complement(14195..15040)
                     /locus_tag="PLF66_14485"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HMZ65301.1"
                     /translation="MLERWEEIRGLVSILLPFHNEGKYIKLAIDSVLNQDYKNFELIC
                     IDDSSTDNSRLILEEYQKVENRIHIYQNPQQLGIALSLNKGIHFARGEFIARIDGDDI
                     WMDQSKLLKQIEFLNKNKDHVVVGTSCRIIDRSNNVIGFLKQTQKNEEIERKIFFRNP
                     FIHSTVCFRKSALREVGLYDPKVLHAEDYDLWLRMKKIGKFSNLEEYITDYRIHRNSI
                     SFINQKRQSLSVIYLQWKYRNRFPKFWLGIFIQILLLFFPTFLFLYLLEFKSGFNNEK
                     NKTRK"
     gene            complement(15003..16058)
                     /locus_tag="PLF66_14490"
     CDS             complement(15003..16058)
                     /locus_tag="PLF66_14490"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HMZ65302.1"
                     /translation="MKLLAQKFHLELNQKIEEYLEQFPNREKANKTMIVKIVIFLALW
                     FFSIGLLYFLRGGYLFYIGTYLLIGFTTVLVIANIGHDASHGNISPISIINKILSFTL
                     NLSGSHFSSWRLKHDEGHHKYTNIQTQDPDIDTGPILRLSPFIQWRWIHRYQFIYIFL
                     IYPLLSLLLILFADFKVVIVNQRIQRNKKIGLIVEMILSKVFYIFYMLGIPIYFSEVG
                     ALPVLTGFIFMHFILGILLAVVFLPSHFNSNSEFYENAKLAYNENSWSIHQIKTTVDI
                     ESKNYLFQFFFGALNLNVLHHIKPRICHTHLFYLQSVLEELCKKYKLNYIQLSLKDAL
                     IGHYKHVRAMGRDSRVG"
     gene            complement(16055..16846)
                     /locus_tag="PLF66_14495"
     CDS             complement(16055..16846)
                     /locus_tag="PLF66_14495"
                     /inference="COORDINATES: protein motif:HMM:NF025871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HMZ65303.1"
                     /translation="MQKKFWKIFKKENRINDSKREIHLEELDTLLARRWKEALSSRFA
                     LKLIKKSRGIDRRIYAMYVIQIYHWAYHTGRTLAITGANPFNTNHEYMDFCYKHAREE
                     NGHELMALSDLREMGVPIQNPAIDMPPPLPGTEVLIAFVRWMATQKNPHRILGYDYWT
                     ENPYKYINSTVLKMAGNFGLKQDQMSFFFKHKNIDIHHEKDIEEILLKVCKTEEDWDM
                     VKETATTTMKILFDMIEDLFDSFEKLKKGQMKKYEILNQMPGIKH"
     gene            complement(16806..18065)
                     /locus_tag="PLF66_14500"
     CDS             complement(16806..18065)
                     /locus_tag="PLF66_14500"
                     /inference="COORDINATES: protein motif:HMM:TIGR01426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HMZ65304.1"
                     /translation="MAHFAFISSPVPGHLNPASSLAQGLNKLGNQTTFINIPDAENFI
                     RSRNLNYSVIGENNFPKGSWHSNWGPASKSADILSTSKTLNVHYKLAETMLSFVPDRI
                     KELKIDALVADHLQFEGSSIAEKMNLPFYTLCPTLPLYSDPEGKYPPPFTDWMYGESI
                     FTRLRNKAAYPIVKFIMKPYLKIINRKRKEWGLDLLKDLSETFSNRIQLSVIPEEFDF
                     PREFLPKNYITIGPLIQESFLGNSISDISRPIIYVSMGTIRNQLPQFFSTLLESLSHL
                     TDYQIILGRGAWNGNPVSIPEFKNLTILDYAPQSAILEKAVLCINHGGANTVLESCAA
                     GVPCLLFPFGEDQYAMAIRMQHKGCGLILNPKSISSRIIQNKVLHLLKEKSYAQNAKS
                     LQEKFTKYKEASHRGAEKILEDFQKRK"
     gene            18388..19527
                     /locus_tag="PLF66_14505"
     CDS             18388..19527
                     /locus_tag="PLF66_14505"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HMZ65305.1"
                     /translation="MSSFLKEDKQVYVERVNESLNVREKHYGKYPLYYLGLIPVSTLD
                     ILTLPIQAPIIFFSMFTNPNVIIYFLPIDRSILDPPESKNTLLKGRDKEYKSKKELEY
                     IEKYPLHYLVKTESINSNTKLKLIKKLLDSGENVNQLDNTDLPVIYYARNSEIIKLLL
                     DSGAQPYGDEFLHDSIAILNPELLSKLISKYSLDINKKDKNGCNVLYNASTRNLQFPE
                     NIELLLDKGATYNRCNTKGDPLSYLLSHNLYRAFNKLISRMKEQNIDLKQELKTEDIL
                     QNNLLHILIQTSLQTSNSKERKISINSIIDSGIDINAKNSQGYTALILASEKDDFEAV
                     EILVQRKADLNIADELNRTALHYAVDDSKVKRLLIKSNTQSGKEK"
     gene            19529..19987
                     /locus_tag="PLF66_14510"
     CDS             19529..19987
                     /locus_tag="PLF66_14510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65306.1"
                     /translation="MYLRKAFRKISRIITYCLLLGIYSCSWTGSGADDYGNNINHWDE
                     RSLRGDKVINKIYSAIQVGIFLRISLCPTNSLNSNCNNTQLITYTTSYFLLKSKISDQ
                     SYYYIESVEKCERKILQGSFLVDPTYIFLSCQLIEQKYYGSKKRRNYILD"
     gene            20093..21592
                     /locus_tag="PLF66_14515"
     CDS             20093..21592
                     /locus_tag="PLF66_14515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004465931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photolyase"
                     /protein_id="HMZ65307.1"
                     /translation="MISSYNQNRNRDTNQKPILENKTYVLYWMQAYRRMHSNHALDYA
                     ISISKELQKELVIYEGLRMDYRWNSKRFHKFILEGMCDNREECEKLEINYWSFVETND
                     NPAKGLLKKIAEKACIIITDDFPSFIIPEQIEKLANKIDCKLIAVDSNSIIPISLYGE
                     KASAARVLRPRVHKLFAEAYIHQAKTKITKKDLFLTNHKSKAPFKEFSGKREDIEKVL
                     SKITFSNHVEPVRDTIGGSKEAAKKLSHFIKKNLNLYGDNRSNPQPQDKAPVTGLSPY
                     LHFGHISVETIVTECLNADRKDKWAPDLLNLDAKGDKDLFFGKNLSLNSFFDELLTWR
                     DIGYLFFFAEREFHKDLKVLPDWLQKNLEKHAKDKREFLYKLKDFEAAETHDPLWNAA
                     QKELVLTGRMHNYMRMLWGKKIIEWTATYKEAFEIAEELNNKYAYDGRNPNSYTGILW
                     CFGLFDRPWFPERNVFGTIRFMSSDSTKKKFKMKEYLEYVDNLDLPRLI"
     gene            complement(21569..21949)
                     /locus_tag="PLF66_14520"
     CDS             complement(21569..21949)
                     /locus_tag="PLF66_14520"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HMZ65308.1"
                     /translation="MEKKILVVEDDEHSGFFLKEALEIHGYKTSIAANGKIALEEYAK
                     NPVPLIITDLEMPFMGGRELISEINKNIAPPLVIVQSAHHEVHSLKDIMKLGVYDYLF
                     KPIQLADLILKVEKAFHLYQTGQI"
     gene            complement(21961..23595)
                     /locus_tag="PLF66_14525"
     CDS             complement(21961..23595)
                     /locus_tag="PLF66_14525"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HMZ65309.1"
                     /translation="MFNPESIHKLKIKQIVGKPVILCVDDELIILSSLSQQLKRKYGV
                     DFTIESVDSPDSALEIVKKCNEDNIDIPVIICDRMMPSMNGDELLILIHKTNPNTRKI
                     LLTGQASTVSISNALNNANLYRYISKPWKNEELTSSIDEALFAYYNDRFLEEKTLELE
                     RNLLLEEIKYKNLVEQMSGAIYLMSMEGKRDFLFQSPQIYSITLYTKEELNYDLWLSR
                     IFPADTENVLKQLDFVLEEQKSALFKYRFYRKDEKLIWIEEDITLLSKNRKPNVIQGI
                     RNDITGRMLAEDKLKQYAIEIEKANQKLLKAFDQAEQANKAKSQFLANITHEFNTPLN
                     SILGYCQLLQMDQIGALNEKQAKFVSYIHESGNHLLSLVNTILDFSKMEAGKVQIEKF
                     EFDLSDLIKEVIHSQEAIGLEKNIKIEMNYDINNRYTIFADRTRIKQVLINLISNAIK
                     FTPEGKSVGINLKGVSDSILLECWDNGIGISEKDLNKIFEPFEQIRNEYTVKTKGSGL
                     GLSIVKSILDLHGFSIGLVSEEGKGSVFTITIPKQK"
     gene            23946..26171
                     /locus_tag="PLF66_14530"
     CDS             23946..26171
                     /locus_tag="PLF66_14530"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HMZ65310.1"
                     /translation="MEGFLDNLSIENADKKLRTIIEYSPQAIVIYDLNLGHFIDANHN
                     AEILFGYDRSELLKLNPIDLSPPEQPNGESSKALKIKKIRETLENGITNFEWIHKDRQ
                     NNIIHCEVYLTKLPDTSHNIILGSIVDITEKVKSREKLKENEEMLRLALGAAKLGIWI
                     WNISNNVITWSDGVYEIFDVSPSEFHGTITEYENLIYKEDRKNVFNSIRWTLTNSVTY
                     SIEHRILTKNNKIKWLEGKGSVERDSNGNPVRMLGSVVDITERKLTEEYLRASEERLS
                     FFYKFTNEAIIILDGNKLRIIDSNPKFRQLFLYDDGEFINLEASSLMIRESWQRIEYS
                     FHKEIEQFESEILARKKSNIIFPALLRVHSFEDRGHKNYILTIIDISTAKEAEQLKKI
                     NKEILLQNEKIENQKLELENAILNLKQTQAKLIQSEKLASLGQLITGIAHEINNPIGA
                     ILASSQNLEDCLEESRRLFPSIIQIFNTMDKESIEEFSNLILESIGKKSFLIGLDRRK
                     KRQEMFTMLQNLNLPTELDDMIVDLGVVSLNQYRNILKNPHIQLLLKFALQEVFANRN
                     ISTIQMSVDRSSKVIYALKNFTHFETDAKKKLNQVSDTIETVLTIYQNHLKKIEIIKS
                     YKYNEAIYCYPDDLIHLWTNIIYNALQAMKFQGILKIEIQKQNDFIEISITDSGEGIP
                     IEIKDKIFEPFFTTKPPGEGSGLGLDIAKKIVDKHMGKIDVKSEKGSTTFTVLLPIFL
                     I"
     gene            complement(26229..26957)
                     /locus_tag="PLF66_14535"
     CDS             complement(26229..26957)
                     /locus_tag="PLF66_14535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015679304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF547 domain-containing protein"
                     /protein_id="HMZ65311.1"
                     /translation="MKSFWKIILFLYLSGLPLLAFDHSHKIYDSILKKYVSVGRVDYK
                     AINKEPSLKIYLDSLSSVSEVEYNSFTKEQKLSFLINAYNAFTIQLILDHYPIKSIRK
                     IGILPLAPWKKEFFKLLGEKRSLGWIEHEKLRKDFEEPRIHFAIVCASIGCPILINEA
                     YIPEKLESQLKLVQTQFLNDSSKNRYDTQFNILYISPIFDWFKEDFTKKSSLIAYLQP
                     FFKDKIATNAKIEYTEYDWNLNEK"
     gene            complement(26954..29089)
                     /locus_tag="PLF66_14540"
     CDS             complement(26954..29089)
                     /locus_tag="PLF66_14540"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HMZ65312.1"
                     /translation="MKIKKIFIVLSIILLIGFARYYNLQDYLTFDSLRANRDLLHNYY
                     LANQISFIVIYILVYVISVAIALPGAAILTLAGGAIFGVLKGVVIVSFASTVGATFSF
                     LISRYLLRDYLKEKFIDKFEAINKGVKKDGIFYLFTLRMIPLFPFFLINVLMGLTDLS
                     VIKFFIVSQVGMLLGTVVYVNAGTQLASINSPRDILSFNMLLSFVALGILPLFARSII
                     NLIQNRKVYNKYKKPKNYDFNLIVIGAGAAGLVTSYIATTVRSRVAIVEKHKMGGDCL
                     NYGCVPSKALLAVSKKIHLQRQFSEYGIRSVKVDFNFIDIMKRVNRVIKEIEPNDSIE
                     RYTKLGVECFTGEAKIVNPYTVSIDGKEYTTKSIVIASGAAPIIPNIKGISDIDYLTS
                     ETLWSLKKLPKRLIVLGGGPIGCELAQAFNRLGSTVTIVEMGSRILPKEDIDVSNFLL
                     DTFQKEGLNLLLSHKAIEFKRKGNQKILICEHNEKKVEIVFDEVILAIGRKARTEGFG
                     LEELGIPITKEGTIETNEYLETIFPNILVCGDAAGPYQFTHTASHQAWYASVNALFGI
                     FKKFKVDYRVIPWTTFTDPEVAHVGLTEMDASKRKLNFEKTVYNIAELDRAIADGEAK
                     GFIKVLTKKGTDKILGVTIVSSHAGEILAEYVLAMKHGIGLNKILGTIHTYPTFSEAN
                     KFAAGNWKKQNKPEKILNLLEKFHTWRRN"
     gene            29179..30240
                     /locus_tag="PLF66_14545"
     CDS             29179..30240
                     /locus_tag="PLF66_14545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020778819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65313.1"
                     /translation="MEVMKNNFIFIRGVGVIGWPLTALFLSLKDLLPNTIIAIEPYQL
                     TKENIYHIEYLCDRGAVFVKSALNDSGNFSSQFNTIPFEEARNLCDIVLDCSPPSIAE
                     KRLSEYGSNLYKNCKGFIAQGSEHKFGKQLLFPDNTSILNSNMDRFYHISTCNTHTLS
                     AIIRIFENLSVAMESIDFVIIRRDADMAKNDPHVTGPLFQIPESENGTHHSRLLNELY
                     QTVQKQYPVTSSAMTVNSPYMHITRFRIEVDRIMKKEQILAEIEKDPLLSITNLISSN
                     MIFSSGRERGDYGRIFSHAVFVEPSLEVNGRVIRGLAATPGDSNVLISTIYAALKVLE
                     VENLDLILQALYQKVIQRV"
     gene            complement(30241..>30628)
                     /locus_tag="PLF66_14550"
     CDS             complement(30241..>30628)
                     /locus_tag="PLF66_14550"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HMZ65314.1"
                     /translation="FHLKIKNFSDFKNSEFKVTTPTITAGVRGTQFMIQEGEDTDSSG
                     NDQIDPGAYVKEGSIEVKPSSSPSAIAVEANQQLVTSQKKLKVEILDEYAREKMKILD
                     TLNVMKEVNYKNLQEQREKNKELIKK"
BASE COUNT        10365 a         5416 c         4803 g        10044 t
ORIGIN      
        1 cgaaaagcga tgggaggaaa gcccgccgct aggcgcaaac ttcatttgaa cgtttagctc
       61 acccaaactc attcattgct tttgagaagc aatgatattg taagatgagt aagtaaaagc
      121 tttctccagc ttttttagtt agagaaagag atcctaaaaa catatcaaga tatatttaaa
      181 taaaccactg aattctagga gtagttttaa gatttttctt agagcctcct aactattttt
      241 ctgataaaat gcctgcattt atttgttgta tattaacaac gaacaggata caattttaaa
      301 aaattaacta ccaatgataa ttttgagaat attagagaat cctaataaat tcaacagcct
      361 acaatgcaac ctggctcaaa gtgataaaaa aaatcggaat atttaaacta acgattcgat
      421 tcaaaattta tttttaaatg cgataggtaa taaacaatga gatctatttt aatactttta
      481 cttttatttt tttcccaatg cttgactgct aaaaaatctc cttttgattt tagatccccc
      541 aatgtattat tgggcagtct atattttagt tttttcgggg attactcggt aagtggaata
      601 atcacaggat ttacaaccgg aacactcatt cttcaaaaca atttagcaaa tgatttaaca
      661 ctctctccca atactattta ttcgtttggt ggactaaaaa gagggaccat ttattcgatc
      721 acaatcaaat caaatccagt tggatacgtt tgttcgattg aaaatttttc tggtataatt
      781 tctgctaacg ttacaaatgc gaatattact tgtgctccaa ttgcaatcac agctctctat
      841 cctcaaaatg gttccaattg gaatgactat atcaataaag gttccggtga tattttctcc
      901 caacccgata cagcttgcaa tacctcaaat acaggtggat acagaacctg cattcatgca
      961 ggagaaattc ggtcctttga actaccttct agatctagct gcacaaattt atcagctaca
     1021 gataatttag atgcactcaa ctggatttgc aaaaccaact caaaaggaag tgtcactttt
     1081 tattcaactg ccttaaaaag aggaaagtat ctttctaatt taatggattg gaatacaagt
     1141 tcatggcaaa atctaacgat tacaataaaa gatggatcca caacgttagc cgtgagcaat
     1201 cctattaagc tatggtcaaa tgccattgat acaacagaac cagcaacccc atccacatct
     1261 ggaacaatct atttatatgg aacaactacc tttccaacga atataattat caatgcaaat
     1321 aaaattgctc tattgataaa tccaaatgga atcaagttga ttcgaagtgc aggaacctgc
     1381 aactcaatag caggaattga tataacaaac agatcctttg tatggttaga agggaacatg
     1441 gactttggaa atagcactca aaactcttat ggtatctcta tcaccaattc taagttttct
     1501 gtttttaaaa atatccgaat tcaaaataca ggagtaaatt gcattactac aggaaatata
     1561 aacaatgcaa tcaatctaaa tggatcttcc aatgattact ttgaagacat atacttggga
     1621 caaacattcg gaacttcacc tgcaaatgga aatgggttat tattaactgc cagtgatgat
     1681 aatgtattta atggaatcag tatttctaat atggcgactg acggcattca actaaattct
     1741 tccaaaggaa atacaattat caatgcgaca gtcatgagtt ctccagcaga atgtgtttta
     1801 atccaatcta gttcaaacaa taataccttt ctgaacttga cgacagtcaa ttcaaacaat
     1861 tatggtgttc gttctgtagg tagcggcaat ggattgtttc aaaactcagt attttttaac
     1921 cacttaaatc atggattaag tctttttact ggatcttcta ttactgttca aaatttaatt
     1981 gcgagcaata ataattcggc tcaggtaaat ttaaatacaa cttcctctta ttttacagga
     2041 atcattaaga cttcatcaac aacatgcatc aacactgcaa gctctggtgg agtaacatct
     2101 acttgccaac ctgtatctcc ctctgacttt tcagctccca tttcaatcag cccttcttct
     2161 aatccttttg ttgggtatag cgctacagac tcaaaaaata caactgcaaa tttaagtggg
     2221 aatccaagtg gcattgcact cgcctctatg acagattggg ttcgatttga ccatttgtat
     2281 aagggaatcg gaaagtttaa ttccgcttct tttccaagca ctgttcatct aggagtttgc
     2341 aactctgcta attgcactat ctgggatttc tctctaaaag cgagcgatac aattcttcga
     2401 aatgcaaata ttccctccgc aagctcttgc ccaaccacta cagtcactca ttctttctct
     2461 acaagtacgg ttactttttt aaggaattca atagagattc taaatgatgg agttggaaat
     2521 gagaatggtc tctgtgaggc aaatgaagat tgtttactca ctccaaatat tggttcctat
     2581 cagggacatg gaaatctagt ttctgcgtct agtctcagtg gctgcaatga catcacttct
     2641 ctcggaatca aactctatca atatgagtct aatggttatt gaatcattca aaattagtct
     2701 ttttccaagg gaagtctttg ttccttctct tttttaattg attatttttg tttgtaggta
     2761 actatacaat ttacaggatt catttttgtt ctaaggagat aaaagatggg tatttttgat
     2821 aaactgaaag gggaatttat tgatgtcatc gaatacaccg atgatagcct ttctggattg
     2881 gtgcatagat ttgagcgaca tggaaatgaa attaaaaacg gagcacagct aaccgttcgt
     2941 gagtcgcaga cagcaatttt tgtaaatgaa ggagaaatgg gagatgtttt taaacctggt
     3001 cgatatgaac taacgacaaa gaacattcca attcttacaa cactcaagag ttggaaatac
     3061 ggtctcaatt ctccattcaa agcagaagtc tatttcgtaa acacaaagat ttatacagga
     3121 ctcacatggg ggactaagaa tcctattatg ttgcgtgatc cagaattcag acaagtgcaa
     3181 gtaagagcat ttggaaccta ctccattcga gtcaatgatc caatcaaatt agtgaaagaa
     3241 gtttctggaa catctagtag ttttgatatt gaaaacgtag aagatcaact cagaagttta
     3301 cttgtgagtg aatttacaga tgcgttagca gaaatgagta ttcctgttct agatgttgct
     3361 cgtaatttta aagaactagg agcaaaaatc caaggttttg taaatgaaga atttggaaaa
     3421 ctaggacttg aggtaacaaa attcattata gaaaatattt ctcttcctcc aaatcttcaa
     3481 aaatttctcg atgaagatac cggtgctgca atggttcgtg atgtaaataa agtgaaccag
     3541 atgaaaatgg gtgatgcaat gctgaacgct tctcaaaatg aaggagctgg tggcgttatg
     3601 ggaatgggga tgggtgctgg tatgggaatg gccatgggtc aacaaatggg tggaatggga
     3661 cagcaacaac aaaccaataa tcctcctcca attccaggcg ctgtagctta ctttgtagcg
     3721 gttaacggtc aacaacaagg acctttcaat ctagaccaac ttaaaacagc tatttccaat
     3781 gggcagataa cacgcgatag cctcgtttgg aaaaatggga tggcaaattg ggtaaaagca
     3841 ggtgataacc cagagacttc tgggctcttt ggatctgttc ctccaccgct cccaccacaa
     3901 caataaattt tggagaatct ctgaatggaa gaagtagcag cagataagaa agaatttcct
     3961 tgctcccaat gtggagcaaa gcttaccttt aagccaggca cagataaaat tgtttgtggc
     4021 tattgtggcc atgagcaagt tgtatcacaa ccaaaagcag aagccactgg cacaacacaa
     4081 attgtagaac atgattttaa tgatgcagta aaaaatgctc gatctgttcc agcaagccaa
     4141 gtagtctcag gtggaaaaga aatacaatgc agtgcctgtg gagccaattc tatcttaaca
     4201 ggtcaatccg atcgctgcgc tttttgtgga tccccactcg tagcagaagt aaaaggacaa
     4261 gaagctatgt ttgtcccaga aagccttctt ccatttcaca ttgataaagc acaagccaaa
     4321 caaaaataca tggattggat caaaggttta tggtttgcgc ctaacgattt aagtaaaaga
     4381 gcaactgcta gcggtatgga cggagtctat ttgccctatt ggacttacga tgcagatacc
     4441 tattctcgct acactggtca aagaggagaa tattattata caacggaatc ttacacagat
     4501 agcaaaggca atacccaaac gagacaagtt agacatacta gatggtatcc cgcttccggt
     4561 tctgtttcag ttgactttga tgatgttttg gtttgcgcct ctaaatctct accacaagaa
     4621 atgatagatg aattagagcc ttgggattta caggaactaa aaccgtatga cgctcgctat
     4681 ctaagtggtt ttgtcacaga acgttataag ataggtctag aggaaggttt ttctgtttca
     4741 aaggaaaaaa tggatggacc aattcgctct aaagtgcgtc aagatattgg cggggatgaa
     4801 caacaaatca catctttaaa tacaacctat agcaacgtta aattcaaaca tttactcctg
     4861 cctttgtgga tttctagtta tcgttataat gaaaaaatct atcgatttgt aattaacgca
     4921 cgaaccggag aagtaaatgg agacagacct tggagttgga ttaagattac tcttgcctcc
     4981 attgctggtc ttgcaattgc aggtgcaatc tattacttca ctcaaaatgc cggatgatct
     5041 ctgcttaggc tcaagcttgg aattgatgtg tatgataaag gaaatgattc tcttaaactt
     5101 ttcgaataga tcaataaacc aagtttgttt cgctctttca agtgatacac ggactaaaag
     5161 caaaacgaat gtccacactt acaataagag ggctagaact tggctcaaaa tcctacagtt
     5221 ctacgcacct ctatctaaga tgagttatta aataagttta tgtataaatc ttcttatata
     5281 gaagggaaga aaaaaccagt tttactttat atcgattctg atcctgtata tttagagaag
     5341 atttattcca ttcttagaaa agaattcgaa aaagattacg agatccaaac agccggttct
     5401 catttaatcg ctcttgaaat tgttcaaacc tgcaatcgaa atctaatcga actaccgatt
     5461 gtcctatgtt cgcagcaaat accggttatc cgcgcttcag aaattattgc aatcatacat
     5521 gaaaccaatc ctagcactcg aaaaataatt tttacagaat ctatttcctc tgaagattta
     5581 ctaacagcta tcaataaagc aaacattttc aaagctcttc caaaagatat tgaggagaaa
     5641 gatttagttt ctataatcca agaaggaata aaatcattct actctgaaaa agatttacga
     5701 gagaagaatg taaaactaga aaaattactt ttttttaata atgatacaaa tcttgcaaat
     5761 tgggaaagat ttcaacaaag actactcgaa tactcaagga agaatctatc actcacagtt
     5821 gcgattatta aaattgaaaa ttatacatac tttcttgaat actttggagc aaagatttac
     5881 aaaaagattc ttttagagct aatcgaaatt atctcctcca cggctctttc tggagaagaa
     5941 atgtttcata ctcagcaaga tgaattttta gttttatcta aaaaacctgt gacagaagac
     6001 ttcatctata agttttcagt tctccgtcta ttattaaaat cacaatacat tgtagtagaa
     6061 aatctttcgt ttcagatttc tctttttatt tctattacaa gtggaaataa aaatttatat
     6121 tcaactgcaa aatcaggtct tatccataga ttcaatggtt ccggtcctac caatgcatct
     6181 atacatataa acgaaatcga atttcataaa gaaaatattc tttgggggca aaaactaaac
     6241 acagcaattc aaaacaaact gattactcct ttttatcaag gaatacaaga taataaaact
     6301 ggagaaatcg tgaagtatga atgcttagtc cgaatgcatg acaatggcga aattatttat
     6361 cctgataaat ttttaaactt agcaagtgcc atgggactca tgaagttaat tacaattatt
     6421 gtaatggata aagcattttc tcattttcag aactcaaaca aatcaatatc cattaacata
     6481 actcaatttg atttagaata caaagacttt gcacaactcg tccaagcaaa attagcttta
     6541 tacaagatta atccttcacg gataacgttt gaaattttag aaaatgtttc ttttggatta
     6601 gaaagccaaa ctttgaaaac attacatgag ctaaaagaaa tcggctgtaa aattgcaatc
     6661 gatgattttg gagttcatta ttccaatcta gcaaggctac ttgaaattca gcccgattat
     6721 ttaaaaatcg atggctggtt cataaaaaat attattcaaa atagaaatgc atatcttgtt
     6781 acaaaagcaa ttatcgaatt atcgcatagt ttaaacgcgg aagtggtagc tgaatttgtt
     6841 acgaatgaag agatccaaaa taaattagta gaaatgggag ttgaattttc tcaaggttat
     6901 ttcataggca agccagaaga gaaaatattt tgattttcat gtattaaaat tatatagatt
     6961 tgagaacgtt ttaatccttc tggatctaaa tttttgaaga agttttgaat aaatttgttt
     7021 ggggctctat ttagaacccc tcttaatttt gccttttcat tttactcgaa tcttttaagt
     7081 ctgctgtttt ctgattgaat aaatgatatg aagcgttatg tcaattacct atttgtattt
     7141 aaaaattaat cgataatcgg ataacctgct gtatttttta gagtagcatc caatcgatca
     7201 atgacttcgc ttaaaatact ttcatttgct gtatctcgat ttagaatagc agctaaatat
     7261 tcatttaatt ctcgaatttc tttgatcgta aatttaatat aataaaaacc atctttatgt
     7321 ttcttggtag acgttagctt tttatgtata gttttgtaag gaacacttgt tccttttaat
     7381 acttctaact catcctcaga aaagtcgatt ggatacattt ctgtgtttag atccattttt
     7441 attctccttt ttaaatttct agaacttcat ctagttgtct tacacaatga ctggcgtgac
     7501 tgcgaaaaac aaattctcct gtcggatcaa cataaatcgt ctttagacca gcgcgattcc
     7561 cagcctctag atcaaaaata taatcaccga ccataactgc atcatttgga gaagcattcc
     7621 atttgtccag taagatatga ataccatccg gactaggctt tggcgtcgcc tgatcgcggc
     7681 taattataaa atcctctcga aaaaattggt ttagaccaga tgcgcgtaat gtctcttctg
     7741 tattgatcaa actatttcga gttaaaatcc ctaaagaaca accgttatcc gaaagagctt
     7801 gtaacaactc tatcgttcca gcggcaaccc ttccgataga agcaagtcta aattcaattt
     7861 cttgtaattt gacatgggct cttttcgcat cttctgcgct cattaaacta atcgcatgta
     7921 aaatatcaat atctagaggt gtcccaagtt ctgttttaat tgctggaaaa tcatgaactg
     7981 gaactgttaa tgttccatcc atatcaaaga tccagcattt tctctctcga attaaattca
     8041 agggacaacc ttaatgcgta ttttagagga tagctctgga ccataagaaa tatgagcccc
     8101 atttgcaaac tgaagagtta catcatattc tccaggctca agttcgatcg aggcttccgt
     8161 ttgtccctta ccgaaatgga aaactttctt ttccacaaat ggtatagtct ttccagaatc
     8221 ataagactcc ccattaataa ttatatgatg atgtccttta ccaagttgaa cttcaccagc
     8281 gggagcaact tccattccct caactcccat cttgaacaca acagggcttt ttacctcttc
     8341 tccgttttga ggagttagaa aaaaaacttt accagtcggt tttttagtgc aggcaacaag
     8401 actaaacaca attaatagta tggaaatata tttaaacata aatttctcct tctcatgttt
     8461 tgtttaacta agattagaat tgagtttttc ttgaaagtaa aaatttattc catttcaatt
     8521 ggaatctctc cttacatact aagcaaactt ggaaatgtgc ctgatcctat tatcattgaa
     8581 aaatgaaatc aagttgagct aaagagaaaa gaaatacttt tcacccatcg ctttttcatt
     8641 ctccctgaac atagtagatc catttactat gaaatctctc ggatcatagg aatagaaaac
     8701 actacctaat taggagaaga catggatact aaaaccatgt ttatcgattt gaattagaaa
     8761 agaaaaaagc cctgcgattg catttaagac aaagcgcagg gagaaaaagt tctatcattc
     8821 gaatctgtaa atgtattacc cgatcgaatc actgtttaca aaaaataaat tctgattcta
     8881 tatttgcaat cgaaccgatt cagattattt tttggtgcca aagtccattg gcggaggagt
     8941 cgtaatatta gaaggaagat ttttgatatc cccttcattt tcaacggtaa aggaagactt
     9001 tggttcatat ccaaatgctt ttgcagttaa tacagttgga aatttacgaa ttgttgtatt
     9061 gtattcttgg actgctttga tataacgtcc tcttgcaact gcgattctat tttcagttcc
     9121 ttctagctgt gcttgtaaat ccctaaagcc ttcatttgct tttagatccg gataacgctc
     9181 cgtaaccaca agaagtcttt ggagtgcaga acctagtgag ttctgggcat cttgaaaatt
     9241 tttgaaagct tctggatctt tcaatgcttc tggagttgct tgtaaacctc ccacactgct
     9301 tcttgctttc gtaatttcta caagcacttc tttttcatga cttgcatagc cctctacaat
     9361 ctttacaaga cctggaatta aatccgcacg tctcttgtat tgatttaaaa tctctgccat
     9421 attcgcattt acttcttcat ctaattcttg gattcgattg tatccacaat ttgcaaacga
     9481 caaaatggat acaaaaaata ttatatttaa aaatcttttt tggctcaatg gtttctcctt
     9541 ttacatacgt tagtcgtaat tgtaggattg tgtaaaaact taaaaaaatc taaccacgaa
     9601 gagcacgaag atcgcgaagg tcaaaaattc ctaggaaatt ttcaactttt cttcgtggcc
     9661 ttcgtgcact tcgtggtgaa aatcttgttc gtacacaacc ccagcaacgt ttccttttaa
     9721 ataacgtcag ttcagtaaag ttaatgaaat attaccacag actcactctg aggcaagtcg
     9781 aatgagcaaa cactgaggac actaataaaa atgaagacaa ttaaaaccta tcagtgttta
     9841 tccgtgttca tccggggtta aaaatcttag acataactca cgttaaatac attccaaaca
     9901 aaaaggataa aaattcaaac taaaaacaaa atggatttta aggtcggagt gctcctgtat
     9961 ttgtatctac aaatgctaaa cgattcttag cagtgccacc gttgatagta gtaaaatctc
    10021 cagcaacaac aattccggag cctattacaa ccattgccgt aacaccattc gaacctccag
    10081 cagcatttgg gtcccatgaa ttaattaccc cactgagact tgtagatccg agatagtttc
    10141 ttacattact aaaaaaagaa cccgcaccag aaaaaattcc acctacatat aaattatttc
    10201 cgtaaacaga aaaggctttt accgcattcc ctgcaccagt tccccctccc atcggatatc
    10261 caccattagc cgcagttgaa ggcactagtc caccaaaatt ataaataaca aggttgcata
    10321 gatttccacc gaaggcacca ccgacaaaga gaacaccacc tgttagtaat aatgcattca
    10381 ctggaaatgt agtggctgtc ccacaccaac cagccagccc attccctgtt gtcctatcaa
    10441 ccgccgcaag atgagtaaaa gtttgtcccc caatagttat agaagtaaaa cttcctccta
    10501 aatatacgtt agttgcatcg taagcaattg cattgatcgt tgcagtattt gcatttggat
    10561 tccaagcagt tgcgtttcca gtatttaaat ctatctctgc aattcgattt ctagcctgcc
    10621 ctcctatggt agtaaatgcc cctcctacat aagcagtcgt tccattttga acaattgcat
    10681 tgatttgacc actagcattt ggattccaag gagtggcagc acctgttctt ggatcgatag
    10741 ccgccccatt gtttctaggt tgtccaccag cattcgtaaa acttccgcca atataaataa
    10801 gtccattata agctaataga gctgtcacgg ctccatttgt tcccatattt acactcgatt
    10861 catttccagt taccaaatca atcacgccta aattgtttct actagaagct ccaagtgtag
    10921 taaaatttcc tcctacaaaa agaaagttac aggaataagc taacgcactg acagtatttg
    10981 ttccaccgga gacccccacc tttacaaagg caggctgtgt ttgaaaagat gtgctacttg
    11041 tataagtcga tgcaagtgta attccagtgg tatcttttac tgccgaagta actttgactt
    11101 ggattgtgta ggttgtttcc acacaaagag gaattgtgag agtgatattc gcagcaagtg
    11161 aatcaggaga aagtgtaact gttcccccaa gacaagttgc gaaattatca gtagagattt
    11221 gaacgttaga tccacaggga ccatttgttc caatgattgt taaggaagat aaatcaatcg
    11281 gtttattaaa taaaattgta attggagttc caattgtata gttgatagaa ttatttatgg
    11341 gagtaatacc aactacattc aaactagttg ctggatttgg aggtgcggga ttattcccaa
    11401 ttgcctcatt tccatccctc aatcgaacta ggtaagatat aaaattttga ttggcaagcc
    11461 ctttaccaat attacaagac tcagcgaaga gatttattat tagaaataga atataaaaaa
    11521 gttttaattt ataattcatg cactaaagca taggcgcaat ctagaaaggg tcaagaattt
    11581 aataaatgaa aatcggtatc atatagaaaa aagaaaaaga ggctacaaaa ggaaaatctt
    11641 aagtagcctc tattcttctt gaatataaaa tagtttaact tgaaagttat tacttctttt
    11701 atataatctt tacttcatac ttccagtctc taaaagtttt atatgccagt tgaatgcttt
    11761 tttgagatca tgcggagtat gttttgtttt agcagttgca aaagaatctc tcaggtattc
    11821 gcgcattgca tctttataag atgggtgaac acatttttct atgatcactt ctgctctctt
    11881 tcttggagcc aaaccacgaa tatccgcaag tccttgttcg gaaacaatga tctgtgtatc
    11941 atgctctgta tgatctacgt gtgataccat tggaacaaag gcagaaattg ctcctccttt
    12001 tgcaactgat ggagccatga aaattgaaac ataggcgtta cgcgcaaaat ccccacttcc
    12061 tccaattccg ttcatcattc ctgttccagt aacatgagta gaatttacgt ttccataaat
    12121 atctgcttct agtgctgtat tcattgcaat gataccaaga cttctcacaa gagctggatg
    12181 attcgaaatc tcttgaggac gaatgatgaa cttatattta agctcagtga tatcatctaa
    12241 aaatttttga tgacctgctt ttgaaaatgt gagtgcagaa gtagaagcac aacgtaattt
    12301 tccgacttct aaaatatcaa agatagagtc ttgtaaaacc tccgtccaca tttcaatttc
    12361 atggaacctt gaatcttttg caatgcagga taacactgcg ttagccacat tccctactcc
    12421 agattgaaaa ggaagatatt cttttggaat tctattcttt ttgatttcaa ataaaataaa
    12481 ttctagaata tgttgtgcaa ttaaaacgct atttgcatcc ggtgctttaa aatctccaga
    12541 atgatcttct aaattagtaa gaacgattcc cttgattttt tctggatcca caactacata
    12601 aggtctacca attctatcat ccactcggct gatatgaagc gctctaccat gaggaggttg
    12661 ctctggtgct gtcagatcat gaaatccttt taattctatt ggatgattcg aatttaattc
    12721 aatataaatt tcctttgcat gatggagata ggttctactc attccagaag aagttgtgag
    12781 ccagatccga ccgtcagacg atacatctac cgcttccaca atagctacgt caattggttc
    12841 taaaattcca tctcgtatgt aatgagctac gtgactcaag tgcatgtcta caaagtctat
    12901 ttctcctgca ttaattcgat ttcgaacatc tggagtagac tggaaaggta gacgcttttt
    12961 catacagcca gttctaccca atacaccatc taactcttcc ccagtagaag cacctgtgta
    13021 taaattaaac caaggatttt ttcctgcttg tttttctttt tcaattctag cggcatatgc
    13081 ctttggaatt acttttggat aaccagcagg cgtaaaccca ctagtagcta tcgttccctg
    13141 gataggcatt ctatttgcca catcttccgc agtcattact ttagattgaa tataaggatt
    13201 catctttacc tcattaaatt tatttctata tttttacaat aactcaagta taaaccaaat
    13261 aacaaaagta atttcccaaa ggattttatt taaggagcat ttggtatagc tattcaaaca
    13321 aaataaagca attaaaggta aagattgcaa gcctgttttg taaaaattaa aaacacctaa
    13381 tactgatatt tatcatatct caaatcttag gtctttcgaa tcactcaaag ctcttagaac
    13441 caaaatggat tgaaaacttc atccattcaa caacttaaaa ttatcgatca gatgttttct
    13501 actcagagag cgaatcaaaa tagatatttc tttttccttt aaccattgca aagcaatctc
    13561 ttccggactt cgattgatct ctacgcccaa tttttttaac attttatttt tttgaatcat
    13621 tccactctta agtggagaat aggcgacagg gaaaatattt ctttccttta aaaaaataat
    13681 tttatcttct tcttttctat ctatataatt caacgggaat tgaactgtta atacttttcc
    13741 tagaagggat tgatttactt tatttatata ctcagtcttt gcattgcaaa tacctacata
    13801 acgtattttt cctgaggaaa tgaatctatc cagcacttca atcgtttcat ccaaggatat
    13861 caatggattt ggccaatgaa tggaataaat atctatataa tctgttttca atctgcgcaa
    13921 actcatctca caagattttt gcaaacgttt cctactcaga tttataaacc aaacttttgt
    13981 tgatagagta attttttctc ttggaacttc tgattgatag agagcttctc ccgttaattt
    14041 ttcggattca ccaaatccat aaatttctgc tgtatctaaa aatctatacc caatactgag
    14101 cgcatttact atcgactctt taaatggagt gttatttttt tgaattactc tcggtaaaat
    14161 tcccgataga ccaagaccta aaacgggaag ttctttattt cctagtttta tttttttcat
    14221 tattaaaccc agatttgaat tccagaagat atagaaataa aaaagtcgga aaaaacaaaa
    14281 gtaaaatctg gataaaaata cctaaccaaa attttggaaa tcggtttcta tatttccatt
    14341 gtaagtagat taccgatagc gattgtcttt tttgatttat gaaagaaata ctatttctat
    14401 gaattctata atccgtaatg tattcctcta aattcgagaa ctttccaatt tttttcattc
    14461 taagccataa atcgtaatct tctgcatgta aaactttggg atcataaaga cctacctccc
    14521 ttagagctga tttccgaaag cagacagttg aatgaataaa tggatttcta aaaaatattt
    14581 ttctttcaat ctcctcattt ttctgagtct gtttcaaaaa tcctatcacg ttattcgacc
    14641 gatctataat tctgcaagat gtcccaacta ctacatgatc tttattttta tttagaaatt
    14701 ctatttgttt taaaagttta gattgatcca tccaaatatc atctccatct attctagcaa
    14761 tgaattctcc tcttgcaaaa tgaattcctt tatttaagga taaggcaata cctaattgct
    14821 gaggattttg atagatatga attcgattct ctaccttttg atattcttct agaattaatc
    14881 tgctattgtc tgtggaagaa tcgtctatac aaatcaactc aaaattttta taatcctgat
    14941 ttaatacaga atctatggca agttttatat atttcccttc attgtgaaac ggaagaagaa
    15001 tactaaccaa ccctcgaatc tcttcccatc gctctaacat gtttataatg tcctatcaaa
    15061 gcatctttta aactaagttg tatatagttt aatttatatt ttttacacaa ctcttctaaa
    15121 acactttgca aataaaataa atgagtatgg caaatacgag gctttatatg atgcaataca
    15181 tttaaattta atgctccaaa gaaaaattga aaaagataat ttttagattc tatatctact
    15241 gtcgttttaa tttggtgaat tgaccaagaa ttttcattat aagccaattt tgcattttca
    15301 taaaattcag aattagaatt aaaatgagaa ggtaaaaata caactgcaag caaaattccc
    15361 aaaataaaat gcataaaaat aaatccagtg agaacaggaa gagccccaac ctctgaaaaa
    15421 taaattggaa ttccaagcat ataaaatata taaaatactt tactcagaat catctcaacg
    15481 attaaaccta tctttttatt tctttgaatt ctttgattca caatcacaac tttaaaatcg
    15541 gcaaataaaa tcaataaaag agaaagaagt ggataaatca aaaaaatata aatgaactga
    15601 tatcgatgaa tccatctcca ttgaataaat ggagagagtc gaagtatagg acctgtatct
    15661 atatctggat cttgtgtttg aatatttgta tatttatgat gtccttcatc atgttttagt
    15721 ctccaagaag aaaaatgact tccagataga tttaaagtaa aagataatat tttatttatg
    15781 attgaaatag gactgatatt tccatgagaa gcatcatgac cgatattcgc tatcactaaa
    15841 acagttgtaa atccaatgag taaataagtt ccaatgtaaa ataaataccc acctcttaaa
    15901 aaataaagca aacctatcga gaaaaaccaa agtgctagaa agattactat ttttacaatc
    15961 atagtcttat ttgctttctc tcggttcgga aactgctcta gatattcttc aattttctga
    16021 tttaattcta aatgaaattt ttgagccaac aatttcaatg tttaatacct ggcatctgat
    16081 ttagaatttc atattttttc atttgtccct tctttaattt ttcaaaagag tcaaataaat
    16141 cttcaatcat atcaaagaga attttcattg tggtagtcgc tgtctctttt accatatccc
    16201 aatcttcttc tgttttacaa accttcagca aaatttcttc aatatccttt tcgtgatgaa
    16261 tatcaatatt tttatgttta aaaaagaaac tcatctgatc ttgctttaaa ccaaaatttc
    16321 ctgccatttt taaaacagtt gaattaatat acttatatgg gttctctgtc caataatcat
    16381 atcctagaat tcgatgtgga tttttttgtg tcgccatcca tcgaacaaat gcaattaaaa
    16441 cttcagtccc tggaagtgga ggcggcatat cgatagctgg attttgaatt gggactccca
    16501 tttctcttaa atcggaaaga gccattaatt catgtccatt ttcttctctt gcatgtttat
    16561 aacagaaatc catatattca tgatttgtat taaatggatt tgctcccgta atggcaagtg
    16621 ttctacctgt atgataagcc caatgataaa tctgaattac atacatagcg taaatcctac
    16681 gatcaatacc tctacttttt ttgataagct ttagcgcaaa tcgactagag agcgcttctt
    16741 tccatcgtct agctaataac gtatccaact cttctaaatg aatttctctt ttgctatcat
    16801 tgatcctatt ttcttttttg aaaatcttcc agaatttttt ctgcaccacg atgcgatgcc
    16861 tccttatact ttgtaaattt ttcttgtaaa ctttttgcat tttgtgcata agacttttct
    16921 tttaataaat gtaaaacctt gttctgaatg atcctactag agattgattt tggatttaaa
    16981 atcaaaccgc agcctttgtg ctgcatacga atcgccatcg cgtattgatc ctctccaaag
    17041 ggaaaaagta ggcatggaac tccagcagca cagctttcca aaactgtatt tgctcctcca
    17101 tgattgatac aaagaacagc tttttctaat atagctgatt gcggagcata gtcgagtatg
    17161 gttaaatttt taaactcggg tatggatact ggatttccat tccaagctcc tcttcctaaa
    17221 ataatttggt aatcagtcaa atgactcaat gattctaata aagtagaaaa aaattgaggc
    17281 aattgatttc gaattgttcc catcgaaaca taaatgattg gtctagatat atcgcttatt
    17341 gaatttccta aaaaagattc ttgaatcaaa ggaccaatag taatataatt ctttggtaaa
    17401 aattctcttg gaaaatcaaa ctcctcaggg ataacgctta actgaattcg attcgaaaaa
    17461 gtttcagaca aatccttgag taagtctaat ccccattcct ttctctttcg attgataatt
    17521 tttaaataag gtttcataat aaattttact attgggtagg ctgctttatt tcttagcctt
    17581 gtaaaaatgc tttcgccata catccaatct gtaaaaggag gaggatactt tccttctgga
    17641 tcggaataga gtggtagtgt tgggcaaaga gtataaaaag gtagattcat tttttctgca
    17701 atactagaac cttcaaattg aagatgatct gcaaccaatg catcaatttt taattctttg
    17761 attcgatctg gaacaaaaga aagcattgtc tctgctaatt tatagtgaac gttaagggtt
    17821 ttacttgtgg ataatatatc tgcactttta gaagcaggtc cccaattaga atgccaactt
    17881 ccttttggaa aattattttc accaatcact gaataattta aatttctaga tcgtataaaa
    17941 ttttctgcat caggaatatt tataaatgta gtttgatttc ctagtttatt caaaccttgt
    18001 gcgagtgaac ttgcagggtt taaatgccca ggaacgggtg atgaaatgaa tgcaaaatga
    18061 gccattgaat attcttttta aaacaccttt aaataaagat ttatggaaaa cactaaatct
    18121 ttcaaatcca ctattacaat atattttatt ttgactctat taaataaaag tcttttctaa
    18181 ttcgatcgca ttagttaaaa ttttcttgga gtattgactt tcttttgttt tcaaattaaa
    18241 atagaaaaat tataaaaata tccgcaaaag ggatttatgt ttaggaaaat attaaaattc
    18301 atttggagga aaaatattaa attacttccc ttaagccttg ctcttttata tttgaattgt
    18361 tttgcaaata atgcttataa tgcgaaaatg agtagcttcc tcaaggaaga taaacaagtc
    18421 tatgtcgaaa gagtaaatga aagtttgaat gtaagagaga aacattatgg aaaatatcca
    18481 ttatactatc taggattaat tccagttagc acattggata tactcacatt gccaatacag
    18541 gctcccataa tattcttttc aatgtttaca aatcccaatg tgataatata ttttcttcct
    18601 atagacagaa gtattttaga tcctcctgaa tctaaaaata cacttttgaa agggagagat
    18661 aaagaatata aaagtaaaaa agagttagaa tatattgaaa aatatccact tcattattta
    18721 gttaagacag aatctataaa tagtaatact aaactgaaac taataaaaaa acttttagac
    18781 tctggggaga atgtaaatca attggataat actgatttac ctgtaattta ttatgccaga
    18841 aattcagaaa taattaagct tcttctagat tctggagcgc aaccatatgg tgatgaattt
    18901 ctccatgact ctatcgcaat tttaaatcca gaattacttt ccaaattgat ttcaaagtat
    18961 tcccttgata taaacaaaaa agataaaaat ggatgcaacg ttttgtataa cgcatctacg
    19021 aggaatttac aatttcctga aaatatagaa ttacttttag ataaaggcgc aacatataat
    19081 aggtgcaata ctaaaggcga tcctttatct tatttactct cacataactt gtatagagct
    19141 tttaataaat taatttctag aatgaaagaa caaaatatag atctcaagca agaattaaag
    19201 acagaagata tacttcaaaa taaccttttg cacattttaa tacaaacaag tttgcaaact
    19261 tcaaactcaa aggaaagaaa gatttctatc aattctatta ttgattcagg tatagatata
    19321 aatgcaaaaa atagtcaagg ctacaccgct ttaatccttg catccgaaaa agacgatttt
    19381 gaggcggttg aaattttggt tcagaggaaa gcggatttaa atattgctga tgagttgaat
    19441 cgaactgcat tacactatgc agttgatgat tcaaaagtaa aacgtttatt aatcaaatca
    19501 aacactcaaa gcggaaaaga gaaatgatat gtatctaaga aaagcttttc gaaaaataag
    19561 tagaatcata acatattgtc ttcttctcgg catttattct tgttcttgga cagggtcagg
    19621 tgcagatgat tacggaaata atatcaatca ctgggatgag agatccttga gaggagacaa
    19681 agttataaac aaaatttatt ctgcaataca agttggtatt ttcttacgaa tcagtctttg
    19741 cccgacgaat tctctaaatt ctaattgtaa caatacacag ttgataactt atacaacttc
    19801 ttacttcttg ttaaaatcaa aaattagcga tcagagctac tattacattg agtctgtaga
    19861 gaaatgtgaa aggaaaattc tacaaggttc ttttttggta gaccctactt atatatttct
    19921 ttcctgtcag cttattgagc aaaaatatta tggaagcaaa aaaaggcgta attatattct
    19981 ggattgaaat aaaagaggaa ggtccacaca ttcagattat taaataccaa tattttctca
    20041 taaacagaaa ataacagtat ctatataacc catttttaaa acactgaacc atatgatttc
    20101 cagttacaat caaaatcgaa acagagatac aaatcaaaaa ccaattttag aaaataaaac
    20161 ttacgttctg tattggatgc aagcgtatcg ccgaatgcat tcaaaccatg cacttgacta
    20221 cgcaatttct atttcaaagg aattacaaaa agaattagtc atttacgagg ggttaagaat
    20281 ggattaccgg tggaactcaa agcgatttca taaattcatt ctagaaggaa tgtgcgacaa
    20341 tagagaagaa tgtgaaaaat tagaaatcaa ttattggagc tttgttgaaa caaatgacaa
    20401 tccagcaaag ggacttttaa aaaaaatagc agagaaagcc tgtattataa tcacagatga
    20461 ttttccttcc tttatcattc cagaacaaat agaaaaactc gcaaataaga ttgattgtaa
    20521 attaatagca gtagattcca atagcataat accgattagc ctctacggag aaaaagcatc
    20581 agccgctaga gtgctcagac cgagagttca taaactcttc gccgaagcgt atatccatca
    20641 ggcaaaaact aaaattacaa aaaaagattt gtttttaaca aatcataaat caaaggctcc
    20701 tttcaaagaa tttagtggta agagagaaga catagaaaaa gtcttatcta aaataacttt
    20761 ttccaatcat gtagaaccag taagggatac gattggagga agtaaagaag cggcaaaaaa
    20821 gttaagtcat ttcataaaaa agaatttaaa cttatatggg gataatcgtt ctaacccaca
    20881 accacaagat aaagctcccg taactggact ttctccctat ttacattttg gtcatatcag
    20941 tgtagaaact atcgttacag aatgtttaaa tgccgatcga aaagacaaat gggcgcctga
    21001 tttattaaac ttagatgcaa aaggagataa ggatttattc tttggaaaaa acttaagttt
    21061 aaattctttc tttgatgagc tactcacttg gcgggatata ggatacttat tttttttcgc
    21121 agaaagagaa tttcataaag atctcaaagt tcttcctgat tggcttcaaa aaaatttaga
    21181 gaagcatgca aaagataaaa gagaatttct atacaaatta aaagactttg aagctgctga
    21241 gacacacgac ccactctgga atgcagctca aaaggaatta gttttaacag gtagaatgca
    21301 taattatatg agaatgctct ggggcaagaa aatcatagaa tggacagcaa cttataaaga
    21361 agcttttgaa atcgcagaag agttaaacaa taaatatgca tacgatggac gaaacccaaa
    21421 ctcctataca gggatcctat ggtgttttgg actttttgat agaccttggt ttcccgaaag
    21481 aaatgttttt ggaactattc gttttatgtc ttccgattca actaaaaaga aatttaaaat
    21541 gaaagaatat ttagaatatg tggataattt agatttgccc cgtttgatat agatgaaaag
    21601 ctttttcgac tttcaaaatt aaatctgcca attgaattgg tttaaaaaga tagtcgtata
    21661 cacctaattt cataatatct ttcaaggaat gcacctcatg gtgagcactc tgcacaatca
    21721 ctagaggagg tgcaatattt ttattaatct ctgaaattaa ttctcttcct cccataaatg
    21781 gcatttctaa atctgttata attaaaggaa ctgggttttt ggcatattct tctaatgcga
    21841 ttttaccgtt agctgctatt gaggtcttgt atccgtgaat ttcgagtgct tcttttaaga
    21901 agaatccaga atgctcatcg tcttcgacta ctaaaatttt tttttccatt tttaattttc
    21961 ctatttttgt ttgggaatcg tgattgtaaa tacacttccc tttccttctt cacttacaag
    22021 tccaatcgaa aacccatgca aatctaaaat actctttaca atcgaaagac cgagtcccga
    22081 tcctttcgtt ttaacagtat attcatttcg aatttgttca aatggctcaa atattttatt
    22141 taaatctttc tcggagatac caattccatt gtcccaacac tccaaaagga tagaatctga
    22201 aactcctttt aaatttatac caacactttt tccttcagga gtaaatttaa tagcattgct
    22261 aattaaatta atcaatactt gtttgattct agttctatcc gcaaatatcg tatatcgatt
    22321 gttaatgtca taattcattt caattttaat atttttctct agtcctatag cctcttggga
    22381 atgaattact tcttttatca gatcagataa atcaaattca aacttttcta tttgaacctt
    22441 tcctgcttcc atcttagaaa aatctaaaat cgtattaacg agagataata agtggttgcc
    22501 actttcatga atataggata caaacttcgc ttgcttttca tttaacgctc caatttgatc
    22561 catttgtaat aattggcaat agcctaatat tgaattgagt ggtgtattaa actcatgtgt
    22621 tatatttgcc aaaaattgag attttgcttt atttgcctgt tctgcttggt caaatgcttt
    22681 taacaatttc tggtttgcct tttctatttc aatggcatac tgctttagtt tatcttctgc
    22741 tagcatcctg ccagtaatat catttctaat tccttgaatt acattcggtt tacgattctt
    22801 agagagaaga gttatatctt cttcgatcca aattaatttt tcatcctttc gataaaaacg
    22861 atatttaaat agagcacttt tttgttcttc caaaacgaaa tcgagttgtt tcaaaacatt
    22921 ttcagtatca gcagggaaga ttctacttaa ccataaatca tagttcaatt cttcttttgt
    22981 atataaagtg atagaataaa tctgtggact ctgaaaaaga aaatcacgct tcccttccat
    23041 tgacattaaa taaatagcac cactcatttg ttctactagg ttcttatatt taatctcttc
    23101 taagagaaga tttctctcta attctaaagt cttttcttct aaaaatctat cattgtaata
    23161 agcaaacaaa gcctcatcta tagaggacgt taactcttcg ttcttccaag gctttgagat
    23221 atatcgatat aaattcgcat tatttagagc gttactaatg cttactgttg atgcttgacc
    23281 agtgagtaga atttttcttg tattgggatt tgttttatgg attagaatta gtagttcatc
    23341 tccattcata ctaggcatca ttcgatcaca aataattacg ggtatatcaa tattatcctc
    23401 attacatttt ttaacaatct ctaatgctga atcgggagag tcaaccgact caattgtaaa
    23461 atcaactcca tattttcttt tgagttgttg cgataaagag gatagtatta ttaattcatc
    23521 atctacgcat aagattacag gttttccaac gatctgttta atttttaact tatgtataga
    23581 ttcagggtta aacataggga tacttacttt ttacaaaatc attcttacta gaaaagcttt
    23641 ttttatccag tccaattact tttattttta ttgtattttc tttattttcc tagatttact
    23701 atacatccta aatatactga aaagaattaa atgaaattag ctgaatgaaa ctttaaaatt
    23761 atatttttat cttacaaaac cagactattt aaattgatct tttttcttat gagtcgctag
    23821 tttttaaaaa atcatctcac tttccttaat ttccctaatt tgcctattgc ctcttactaa
    23881 atattcttgc agaatcattt atacctgaat acattgtgct attgtaaaca gggtttaaag
    23941 agtgtatgga aggtttttta gacaatctat caattgagaa tgcagataaa aaactaagaa
    24001 caatcattga gtattcacca caagcgattg ttatttacga tttaaatctt ggccatttca
    24061 ttgatgcaaa tcataacgca gagattctct ttggttatga tagatccgaa ctattaaaac
    24121 taaacccaat tgatctaagc ccgcccgaac aacccaatgg agaatcctcc aaagctttaa
    24181 aaatcaaaaa gattcgagaa actctagaaa atggaattac taattttgaa tggatacata
    24241 aagacaggca aaataatatt atccactgtg aggtatatct tacaaaacta ccggatacat
    24301 cccataacat tattttaggt agcatagtag atataacaga aaaagtaaag tccagagaaa
    24361 aattaaaaga aaacgaagag atgttaagac ttgcgttagg cgcagcgaaa ctaggaatct
    24421 ggatttggaa tatttctaat aatgtaatca cgtggtctga tggtgtttat gaaatttttg
    24481 atgtaagtcc atcagaattc catggaacta ttactgaata cgaaaacctg atttataagg
    24541 aagataggaa aaatgtattt aattcaattc gatggacttt aacgaatagc gtaacttact
    24601 ccattgaaca tagaatttta actaaaaata ataagattaa atggctagaa ggaaaaggat
    24661 cagtagaaag agattcaaat ggaaatcctg tccgaatgct tggaagcgtt gtagacataa
    24721 cagagagaaa attaactgaa gaatatttaa gagcaagtga agagcgacta tcgtttttct
    24781 ataaattcac caatgaagca atcatcattc tagatggaaa taaactcaga ataatcgata
    24841 gtaatcctaa atttcgccaa ttatttttat atgatgatgg ggaatttata aacttagaag
    24901 caagtagttt aatgatacgt gagtcttggc aaaggattga atattccttt cataaagaaa
    24961 tcgaacaatt tgaatcagaa atattagccc gaaaaaaatc gaatattatt ttccctgctc
    25021 ttcttagagt tcattctttt gaagacaggg gtcacaaaaa ttatattctc acaattattg
    25081 atatatcaac agcaaaggaa gcggagcaat taaagaaaat aaataaagaa atacttttac
    25141 aaaatgaaaa gattgaaaat caaaaactag aattagaaaa tgctatttta aatttgaaac
    25201 aaacacaagc aaagttgatt caatccgaaa agttagcaag ccttggacaa ctgattactg
    25261 gtatagcaca tgagatcaat aatccaatcg gtgccatcct agcatctagc cagaatctag
    25321 aagattgttt agaagaatcg agaagattat ttccttctat cattcagata ttcaacacaa
    25381 tggataaaga aagtattgaa gaattttcga atttgatttt agaatccatc ggtaaaaaat
    25441 cttttttaat tggtcttgat cgaagaaaaa aaagacaaga aatgtttacg atgctacaaa
    25501 atctaaatct tccaacagag ttggatgata tgattgtaga tttaggagta gtatctctta
    25561 atcaatatag aaatatttta aaaaatcctc atattcagtt actcttaaaa ttcgccttac
    25621 aagaagtttt tgcaaataga aatatttcaa caatccaaat gtccgtagac agatcctcca
    25681 aagtaattta tgctctgaaa aattttaccc attttgaaac ggatgcaaag aagaaattaa
    25741 atcaggtatc tgatacaatt gaaacagttt taactattta tcaaaatcat ctaaagaaaa
    25801 tcgagattat aaaatcttac aaatacaatg aggctattta ctgttatccg gatgacttga
    25861 ttcatctctg gacaaatata atctacaatg ctttgcaagc tatgaaattt cagggaatat
    25921 taaaaataga aattcaaaag caaaatgatt tcatagaaat ttctattaca gattcaggag
    25981 agggcattcc gattgaaatc aaagataaaa tttttgaacc attttttaca accaagcctc
    26041 cgggagaagg aagcggacta ggactcgaca ttgcaaaaaa aattgtagat aaacatatgg
    26101 gaaaaataga tgttaagtca gaaaaaggct ctacgacttt cactgtatta cttcccattt
    26161 ttttaattta atttactttt ccgaaacgaa tccaagtcat tttgcttata gagcgattga
    26221 ctcccttatt atttttcatt taagttccag tcatactctg tatattctat tttagcgtta
    26281 gtcgctattt tatctttaaa aaatggttgc aaataagcaa ttaaactaga tttctttgta
    26341 aaatcttctt taaaccaatc aaaaatcggt gatatataaa gtatgttgaa ctgtgtatca
    26401 tatctatttt tactagaatc atttaaaaac tgagtttgaa ctagttttaa ttgagattct
    26461 aatttttcag gaatatacgc ttcatttatt aagataggac atccgataga agcacataca
    26521 atcgcaaaat gaattcgtgg ctcctcaaag tctttacgta atttttcatg ctctatccaa
    26581 ccaagacttc ttttttcccc tagcaattta aaaaattcct ttttccaagg ggctaatggc
    26641 aatataccta ttttacgaat gcttttaatt ggataatgat ctaaaattaa ctgaatcgta
    26701 aaagcattat aggcattaat tagaaaggat aatttctgct cctttgtaaa gctattatac
    26761 tcaacctcag agacagagga aagtgaatcc aaatatattt ttaagctcgg ctccttgtta
    26821 attgctttgt aatcgactct accgacagat acatatttct ttaagattga atcatatatt
    26881 ttatgactat gatcaaaggc taataacggt aatccactca aatataaaaa tagaattatt
    26941 ttccaaaatg atttcaattt ctcctccatg tatgaaattt ttccaatagg tttaatattt
    27001 tttctggttt gttttgtttt ttccaatttc ccgctgcaaa tttatttgct tcggaaaagg
    27061 taggataagt atgaatggtt cctaaaattt tatttaggcc aattccatgc ttcatagcca
    27121 aaacatattc ggcgagtatt tccccagcat gagaggagac aatcgttact cctaaaatct
    27181 tatctgttcc tttttttgtt aggactttta taaatccttt tgcctctccg tcggctatcg
    27241 ctctatctaa ttctgcaata ttataaactg ttttttcgaa attcaatttt cttttactag
    27301 catccatttc agttaaacca acatgagcta cttcaggatc tgtaaatgta gtccaaggta
    27361 tcacacgata gtctacttta aactttttaa agattccaaa gagagcattg acagaagcat
    27421 accatgcttg atgggaagca gtatgagtaa attgatacgg acctgcagca tccccacaaa
    27481 ctagaatatt tggaaatata gtttctaagt attcatttgt ttctattgtt ccttcttttg
    27541 tgatagggat tcctagttcc tctaacccga agccttccgt gcgagccttt ctaccaatag
    27601 ccaaaataac ttcatcaaaa acgatctcta cttttttctc attgtgctca cagataagta
    27661 ttttttggtt tccttttctt ttaaactcaa ttgctttatg agatagcaat agattgagac
    27721 cttctttttg aaaagtatct agtaagaaat tggaaacatc tatatcttct tttggaagaa
    27781 ttctgcttcc catttctact atcgtaacag ttgatcctag acgattaaat gcttgtgcca
    27841 attcacaacc gataggtcct cctcccaaaa caatcaatct cttaggtaat tttttaagac
    27901 tccaaagagt ttcagaagtt aagtagtcaa tatcagaaat tccttttata tttggaatta
    27961 taggagctgc tcctgatgca atgacaatcg atttggtggt atattctttt ccatcaatag
    28021 agactgtata gggattaaca attttcgcct caccagtaaa acattcaacc ccaagtttag
    28081 tataacgttc gatagaatca tttggctcaa tctcttttat gactctattt actcttttca
    28141 ttatatcaat aaagttaaaa tcaactttca ctgatctgat cccatactca gaaaactgcc
    28201 tttgaagatg aatttttttt gaaacagcaa gcaatgcttt agacggaacg cagccataat
    28261 ttagacaatc cccgcccatc ttgtgcttct ctactattgc aacgcgagat cgaacagtcg
    28321 tagcaatata agatgtaacg agtccagctg cccctgctcc aatgactatt aaattgaaat
    28381 cgtaattttt tggtttttta tatttattat aaacctttcg attttgaatt agattgataa
    28441 tagatcgagc aaataacggt aatattccta atgctacaaa agaaagaagc atattgaatg
    28501 atagaatgtc tcttggcgaa ttgatactag ccaattgtgt tcccgcattt acataaacca
    28561 ctgttcccaa aagcatacca acctgactca caataaaaaa ctttattaca gataaatcag
    28621 ttaagcccat taaaacgtta attaaaaaaa atgggaatag aggaatcatt cttagagtaa
    28681 ataaataaaa gatcccatcc ttttttactc ctttatttat agcttcaaat ttatctatga
    28741 atttctcttt tagataatct ctaagtaaat atctcgatat taaaaaactg aatgtagcac
    28801 caacagtgct tgcaaaggaa acaataacta ctcctttcaa aactccaaag attgctccac
    28861 cagctaacgt taatattgct gctccgggta atgcaatcgc aacagaaatt acataaacaa
    28921 gtatataaat tactataaag ctgatttgat ttgccaaata ataattatgg agcaaatcac
    28981 gatttgccct caagctatca aaggttaaat aatcctgtaa attataatag cgggcaaacc
    29041 ctattaataa aattatactc aatactataa atatcttttt tattttcata ctattccatt
    29101 ttttaaagat aagttttatt tttaaacaac tcagattaca atctaattct aattgcgaga
    29161 gagcaagcct ttaacattat ggaggtcatg aaaaataatt tcatttttat tcgaggggtc
    29221 ggtgtgattg gctggccgct aactgcctta tttctttcct taaaagattt attgcctaat
    29281 actattattg caatagagcc atatcaattg actaaagaaa acatttatca tattgaatat
    29341 ttatgtgata gaggagcagt ttttgtaaaa tcagcattaa acgattctgg aaatttttct
    29401 tcccaattca atactattcc ctttgaagag gcaagaaacc tctgtgatat tgtcttggac
    29461 tgttctccac cgagcattgc agaaaaacga ctttctgaat acggaagtaa tctatacaaa
    29521 aactgcaaag gtttcatagc ccaaggaagt gaacataaat ttggcaaaca attattattc
    29581 cctgataata cgagtatatt gaattctaat atggaccgct tctatcacat tagcacctgt
    29641 aatacacata cactttctgc aatcatacga atttttgaaa atttatcagt tgctatggag
    29701 tctatcgatt ttgtaattat ccggagagat gccgacatgg ctaaaaatga tcctcatgtg
    29761 acaggacctt tatttcaaat tccagaaagt gaaaatggaa ctcaccattc tagactccta
    29821 aatgaactct atcaaaccgt tcaaaaacaa taccctgtaa catcgagtgc gatgactgtt
    29881 aactctccct acatgcatat aacccgattt agaattgaag tggatcgtat aatgaaaaaa
    29941 gagcaaatcc tagcagaaat agaaaaagat cccctacttt ccattacgaa tttaatctca
    30001 tctaatatga ttttttcatc gggtagagaa aggggagatt atggcagaat cttttcccat
    30061 gctgtttttg tagaaccttc cctcgaagta aatgggaggg tgatacgagg gttagccgct
    30121 acaccaggtg attcgaatgt tctgatttca acgatttatg cagctcttaa agttttggag
    30181 gtagagaacc tagatttaat tttacaggct ctctaccaaa aggtgattca aagagtatga
    30241 ttatttctta attaattctt tatttttttc tctttgttct tgtagatttt tataatttac
    30301 ttctttcata acgtttaacg tatctagaat cttcattttc tcacgagcat attcatctag
    30361 aatttctact tttagttttt tctgagaagt aactaactgc tggttggctt caaccgctat
    30421 tgcggatgga gaactacttg gttttacttc gatagatcct tcttttacat aggcacctgg
    30481 atcaatttga tcattgccag aagaatctgt atcttctcct tcttgaatca taaactgagt
    30541 tcctctaacg cctgcagtga ttgtaggagt agtcacttta aactccgaat ttttaaaatc
    30601 actaaaattt ttaattttta aatgaaaa
//
LOCUS       DAOQOS010000064        30463 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_667_length_30463_cov_6.358129, whole genome shotgun sequence.
ACCESSION   DAOQOS010000064 DAOQOS010000000
VERSION     DAOQOS010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 30463)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30463)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30463
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_667_length_30463_cov_6.358129"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(8..730)
                     /locus_tag="PLF66_14555"
     CDS             complement(8..730)
                     /locus_tag="PLF66_14555"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HMZ65315.1"
                     /translation="MLAERKEYIQDGNNKIRISNYEKTKFDVSLISINFTDSIKGESE
                     YELNDPQMSELAESIKTVGLIQPIVLRKIGILKAITSKGQEIKQEGYEVVAGRRRFTA
                     ISKILKWKTVDAIVRDYDDSIDTLAVQFQENEERKAWTDHNYATAIKMLREKKPGIST
                     IEIAKTFNKTPDWVKKKNDHIRVIDSFEKVNGEGATSHLAELSTSEINATGQVKDETA
                     RVDLITDIFSLLRNVKELKVSL"
     gene            complement(730..897)
                     /locus_tag="PLF66_14560"
     CDS             complement(730..897)
                     /locus_tag="PLF66_14560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65316.1"
                     /translation="MPKKTELEEFIDYAFYNNGINQDALHKSYREFREKKLKEKIDKL
                     ETQKQNLIGAL"
     gene            complement(890..1210)
                     /locus_tag="PLF66_14565"
     CDS             complement(890..1210)
                     /locus_tag="PLF66_14565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65317.1"
                     /translation="MNLFQDYKDYFSELNDSFKKTKNGFEKIISNRTKSSKKEKIFSN
                     SLARIERLVQLIEDDISDIEDVINSNMSVAKNQQLERALDEIDLFIESEKRKIYVYAE
                     KANA"
     gene            complement(1212..1724)
                     /locus_tag="PLF66_14570"
     CDS             complement(1212..1724)
                     /locus_tag="PLF66_14570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65318.1"
                     /translation="MIHLINGAPNIVLIPKKEFNGTIIKGGFIFNTRSEDDFKADIHL
                     SPGMQILQLGNHSEYTFKFKDPVTYEGFAWYQFPENEKPTPILLFQDDAERNISKNGA
                     AYSYKALIYNSRKSLYCITGENREVDFRNVYMNFIQLFVPGIKEEKPVEVNEPKPVNS
                     TQLLLFGRGY"
     gene            complement(1721..2023)
                     /locus_tag="PLF66_14575"
     CDS             complement(1721..2023)
                     /locus_tag="PLF66_14575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65319.1"
                     /translation="MNENEVGTIICCLNCVHGTRKKSDPYFVFCNNSSSPARGNWMTI
                     ESSCNEFEDKPLKKLATGFILGAFNAPFAGIFFMPKDPVKLFLKLFPGFKFHRGES"
     gene            complement(2020..2439)
                     /locus_tag="PLF66_14580"
     CDS             complement(2020..2439)
                     /locus_tag="PLF66_14580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65320.1"
                     /translation="METVTQEEEKSQEVKPRFKVGDKVYIQNETNPMILESALITDVY
                     ISESLNYEHKRKIYEFIKYKPEKIETVFSINYKVYVMNEESEFFSREMHEKKCYISKE
                     EFFKNIELTDFIEDLQKAEAERQAEIAEYYKQEGESI"
     gene            complement(2513..3394)
                     /locus_tag="PLF66_14585"
     CDS             complement(2513..3394)
                     /locus_tag="PLF66_14585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65321.1"
                     /translation="MIDFAKVPDIDPDIHPIGNYRKITQWMVDFCEGDFVEAILLSFF
                     VAMYHNQRKYFLEAIRKNQIFKQYGLTKKESESIELIYSYRQISGYIFNATKKYEVLK
                     ARIDSLAARGVITIKKALTVRDKQLLGIDTRLSTDRTNVYLFHPTPLIEYLQPYLNLQ
                     NDHDNEIQNSINLLEGKGILQKTKDGYFLPFQNKKTSGATSHLKKFQTQQAAIAPAKD
                     KADIIADLKKHSKKNFSAVTNERILKSKILIQNEKIIITIGEYGMSVSDKKTIEKYFS
                     ELEETKSFKVVFEDNDN"
     gene            complement(3391..3690)
                     /locus_tag="PLF66_14590"
     CDS             complement(3391..3690)
                     /locus_tag="PLF66_14590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65322.1"
                     /translation="MNTNQTEIVDLESTKILKKLSAVLEDEKNHQIEELSFIIRMNRK
                     EMAEIREKYGLTRTEFFKIPDPDGIHYFKLRTQNFLMESTIKTITMFTRKLRGQK"
     gene            complement(3687..4559)
                     /locus_tag="PLF66_14595"
     CDS             complement(3687..4559)
                     /locus_tag="PLF66_14595"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HMZ65323.1"
                     /translation="MKNLQQTSIDQYNRKVKKFNDWLSQKKYKRITFKILRQYFVYLR
                     FTLKFSEANLHAHKFAIFKSIQEYYEDKHDYKTVAVFREFFRSLEYRIRPAVLDLNQL
                     PTLKEVVQIMAYASQKEFCLIFFLYITGFRNHELRKILLTDCKVNPKKTKLTIIIEGK
                     NRKRRAVTIPVWLYSMIRKQYAGKVYLFESKDNKPLQPTTVQFLVREATKRVGIKKIV
                     TPKILRISLLNHIYYSIPGISPDWMRERFGHTDATRERWYRLFTNKDNAQFSKFENKF
                     IKEIKKISGKRLIA"
     gene            5070..6731
                     /locus_tag="PLF66_14600"
     CDS             5070..6731
                     /locus_tag="PLF66_14600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65324.1"
                     /translation="MRKIILIIGIGFLLNCNLFSKKKEDDNKLGILAYLATRTSASED
                     TTNDGIYQDFSVTSGTTVNATYNASALSVQDLNLAITSGTDNNTKVEVSVVDPDGLEQ
                     SYFRNGDGAMTVNFTPTKTGTYRVNFKNNSERSVSINNSSVSGGTATSSAGRSNTNAK
                     FKDIFLKAYVGLSTRCNTFNTQVTAASGSFYVQPIVFLGKIGTGKKVSDITTATIKLA
                     YGGTTLTLQRLDDMNANLYDNPNNPSNSIPSSDKANKIKYIKSYYQSLFGAAGELYTI
                     GDTFNKSTGSTCSSASTLPLGTSNFGNSVITMTLVDSANGINESFKIRPVVNAIDFSQ
                     FFKKSDGTAFNDFSTCSYDKTTGSSASCAKTFSLTDPPYVQLSSQALDTSVSAPTRLL
                     FFGANYPKSYYTQLISNSVALSNGSMKSITIDGCLNNGGLNSVKLSATDKVYLPLREF
                     NLAKDDISQLKFQPGTYTHLKDFYQGNADLSGSVDLPVCIPSTTQECPTYKTITVNSS
                     KCRVKVDSGITVGAFSSSPTWTTADTNYLLYPDNLGGGLSARITE"
     gene            6871..7410
                     /locus_tag="PLF66_14605"
     CDS             6871..7410
                     /locus_tag="PLF66_14605"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HMZ65325.1"
                     /translation="MNAIKNTTLLEAVAAVIQHEKDCFDFYLDIYEKVKEGSVKDFFK
                     ELAEDVEEHIKLIQEMYNDLSGGVEFPNLKQLTAIHKFDKTHLTKLMRKIDRNTEHAF
                     KDDIDAVQRAMQVSEDAREFASKMKEKFKDPAIKSFFNKLAFIKEEERIMAESQYLFL
                     QQTDKTKYYWEDDSLVNSK"
     gene            7870..9150
                     /locus_tag="PLF66_14610"
     CDS             7870..9150
                     /locus_tag="PLF66_14610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65326.1"
                     /translation="MFNKTIYFVFGIFTNLGEIYLFLFLFTQITSCNFISASKAKTNL
                     DRNLLVAGVAIFSNQSKDDVVQYAQTCKQELGLSAETLAPWNCIDGLEIPITVNGKIP
                     DDDTYRNLMQGNIACDYPSWLQPPEACLNYAFVQERNLSPDVKAVLLCRARDSNLNKT
                     RAQRLLDLKNNPTIETFLNYYRQRTIALIWTNTKTGKTCFFDSRVEYAGYIPSPDDTR
                     YPTYEDLPEPKFLIDANGEALDKSLWQRNAYSVWKNPSFVFNTDQCISCHTASPFITS
                     PWLKQVYQPPRLSSSTPYVIIGDFYNKKSFPLYSVTTEPIYKDGLLEAQVCTSCHRIS
                     NRFECDKLIDYSVGLKSPVSKPENAVLPNLWMPPEVQNTVTTKEDWARKYKSHYDRLK
                     CCCQNPNAINCSYQDINQNSLSTLQRGKGPEVCN"
     gene            9301..10437
                     /locus_tag="PLF66_14615"
     CDS             9301..10437
                     /locus_tag="PLF66_14615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002179795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HMZ65327.1"
                     /translation="MNSMNDWNKIYNEYPVNQELIWLNNCGTTPAGNRAIKDVTRYLE
                     AYSRMGVFNETEKYPTVKRRITEILTSILNSDSEEIGIIHNTSEGMNFISHGLNLQSG
                     DEILLLENEYPSNVYPFQHWEEKGVSLKFTPMANTPKEFLENFKSSLSPKTKVVSLSL
                     VHWCTGMPLPIEEIGNICRERNIELVIDGAQGVGLIPVDVKKMNISYMAFPAWKWLLG
                     PLGLGGIFVAKDRLATMKTIFKGQNSVVNADEYLPYKNQIRSGAERFEYSTSNFTDWV
                     YWKSTLEMLFEIGFSNVQERIYSLQSYLADGLRKKGYTIYSDSYPEVKTGILACEKAG
                     SDSASLVKHLRENKVISAVRLNRVRLAPHIYNSFEQLDKVIELL"
     gene            complement(10608..10781)
                     /locus_tag="PLF66_14620"
     CDS             complement(10608..10781)
                     /locus_tag="PLF66_14620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65328.1"
                     /translation="MKTKKFDTIAMKRKAQEKISKQTEGLSEKELLEYWNQYTLKNKK
                     LQSPKKKNVSRKK"
     gene            complement(10993..11142)
                     /locus_tag="PLF66_14625"
     CDS             complement(10993..11142)
                     /locus_tag="PLF66_14625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65329.1"
                     /translation="MNDRDSVRLSHILDACLELTGIISNFSDVETFLSHRLYQHATVR
                     LLEII"
     gene            complement(11416..11640)
                     /locus_tag="PLF66_14630"
     CDS             complement(11416..11640)
                     /locus_tag="PLF66_14630"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HMZ65330.1"
                     /translation="MNFLLDTHIFLWYINGNEKLKVEYRKIIEDFENTIFISAASLWE
                     IIVKAKMGKLPLPAPYYDYIISQINIHSFS"
     gene            complement(11637..11861)
                     /locus_tag="PLF66_14635"
     CDS             complement(11637..11861)
                     /locus_tag="PLF66_14635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65331.1"
                     /translation="MQPIRIIYSNLPDILEIPKELKNKKAEIIIWPLEQSDEKKLQKR
                     PFGLAKNDFQIPDSFFDPLPNDILIGFGAL"
     gene            complement(11936..13545)
                     /locus_tag="PLF66_14640"
                     /pseudo
     CDS             complement(11936..13545)
                     /locus_tag="PLF66_14640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012476784.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein"
     assembly_gap    13546..13645
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<13646..16693)
                     /locus_tag="PLF66_14645"
     CDS             complement(<13646..16693)
                     /locus_tag="PLF66_14645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004776233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HMZ65332.1"
                     /translation="MTNNQSLFKQENITLDKVAKKSDLEVLLYNFRTVAVSEREKGTY
                     FEELIRTYLKNEPKYKDLYEEVWHYGDWAKQEGMDARDTGIDLVARANGTNEYYAIQC
                     KFYDPDYRVQKSDIDSFFTASGKKPFSHRVIVTTTNNWSEHAEDALLNQTPPVTKIDL
                     YALENSQIDWSKYKSKTKPVFKPKKKLRPHQEIAVKSVLEGLKKEERGKLIMACGTGK
                     TFTSLKIAEKLAGKGKRVLFLVPSLALLSQSLTEWTQESEVPLHSFAVCSDSEVGKKR
                     NKEDDVVNTFAHELQFPATTEPSKLAKEMKSRHDAHHMSVVFSTYHSIDVIGEAQKKY
                     KLEDFDLIVCDEAHRTTGATFGDEDESKFVKVHDGKFIRANKRLYMTATPRIYGEAAK
                     VTAEKENVALCSMDDEKLYGKQLHVITFSQAVQSDLLVDYKVIVLAVDEAHISSRLQK
                     LLADEDNQIKVDDAAKIIGCWKALSKQGAEDLEEDNPMRRAVAFCQVIEYNQNSKTHK
                     VSSKQIAKMFQAVVDSYISSERSRPVSNNTNETINLKCQVEHVDGSMNATEKESKIHW
                     LKRDSEENVCKILSNVRCLSEGVDVPALDAVLFLTPRNSQVDVVQSVGRVMRKAEGKS
                     KGYVILPVVIPAGIPAHEALNDNKTYKVVWQVLQALRSHDDRFDAMINKMELIGPDKS
                     KMEVIAVTDSIAKKTKSGGKSGESGNGNTIGKTGGSRPTPTKTDTQQKLQFDIGEIEI
                     ALYAKVVQKCGNRHHWEDWANDIAKIANTHITRIRTIVENKNNTKERKAFYDFADELR
                     DDLNESITNEEVIEMLAQHLITKPVFDALFSKYEFTKQNSVSRAMDEILSILEKHHIE
                     KEADTLKSFYASVRMRAEGIENAQGRQKIILELYDKFFRNAFPKLTERLGIVYTPVEV
                     VDFIIHSVNDILRSEFGESLGSKNVQILDPFTGTGTFITRLMQSGLLTKEELIQKYKT
                     GLHANEIVLLAYYIAAINIESTYHDLVNTDYTPFTGILLTDSFQ"
     gene            complement(16694..17083)
                     /locus_tag="PLF66_14650"
     CDS             complement(16694..17083)
                     /locus_tag="PLF66_14650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65333.1"
                     /translation="MEIIFYAKNGKQYILEVSESFYKWLALSEFSKMQTSKVYKVDIE
                     GDEAEEIEAVELTEQNRNSYISFFQKCILRESYDLIVKMESSSKLGLLKASSFEGSLE
                     NLQYRIKKFYEFIKCLEDRAWVYMEYV"
     gene            complement(17086..17289)
                     /locus_tag="PLF66_14655"
     CDS             complement(17086..17289)
                     /locus_tag="PLF66_14655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65334.1"
                     /translation="MPYIEIKTREKINIGLAFELIEKGTITAVVTTGTITPNAKKLFD
                     EAGIAWAENIPESEFINVKSMEI"
     gene            complement(17289..17516)
                     /locus_tag="PLF66_14660"
     CDS             complement(17289..17516)
                     /locus_tag="PLF66_14660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65335.1"
                     /translation="MEKPNKPTDEDRMIQKLTEHKNLIGWMLTLFQKENIQSERTYKN
                     DPKGDIYYPDEKDTEKVKEIVRDLNKKYNTK"
     gene            complement(17757..18254)
                     /locus_tag="PLF66_14665"
     CDS             complement(17757..18254)
                     /locus_tag="PLF66_14665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65336.1"
                     /translation="MKLNKQPAIEYELVKRWAIALQLDKYFSLVSEDIYRAENIDNSL
                     ERELPNAEVEMFQFQRAQEIIGTNMAVVTYMVDALQFFEKMSQDNIKKIAVEIALLGA
                     KGFRTDKNDYTLSSIPNTQFSGYKILAYYYVSWKLALPEMLSQLQLPFNDEYKVALTM
                     FKKEK"
     gene            complement(18258..19706)
                     /locus_tag="PLF66_14670"
     CDS             complement(18258..19706)
                     /locus_tag="PLF66_14670"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HMZ65337.1"
                     /translation="MQIIHKIDDFLFNLFPNIKKEGDDKNSLLVEKLEQYYSYGQYKP
                     KVRIENGYVIIDIDISEIQSQEFDYKKAIQYCDTGKFREAEPILKSLIKKNPTNSEYY
                     RILGQVYSEQGNQDEAINCLIDSLRWNSKNGWALLLMGNIFSKFKNDISTAMKYYDQS
                     LKVNPNDYIVITNIGGSLMQQGKFSEAKKYFLHSLEINKEYPNTQYALGIIAEKENNL
                     QTAFMYSIAAAKLCKPQDQIYQISLKQAFEVSERIVQENKETAIIKNYLHKLEFECDK
                     QIELVEDSTISTAAKIEFAENHNRKKHILRYKPSYPTIQHLILHELIHLDFVIQARKE
                     KANLLFISNSENKKEFISSIQSTIDLLTKKEIQQEAIQNYTQALFEGINQQIYNTPID
                     LFIEDLIYSEYPELRSYQFLSIYTFIQEGIRAVTDPKTLEVAPTDLISKSKIFNLVTA
                     LHYKDLFGFDLIAQFKPTNAELEQAKNFLSEY"
     gene            complement(19690..20010)
                     /locus_tag="PLF66_14675"
     CDS             complement(19690..20010)
                     /locus_tag="PLF66_14675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65338.1"
                     /translation="MIEAGKGAQHEIVDILLTRYACYLIAQNGDSRKEEIAFAQNYFA
                     IQTRRAELVEKRILEFERVKAREKLSQTENQLSLSMFFLCDIRFLIDPKLYPSKKLSN
                     ANNT"
     gene            20404..20940
                     /locus_tag="PLF66_14680"
     CDS             20404..20940
                     /locus_tag="PLF66_14680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65339.1"
                     /translation="MNLSIAPTILGILLFGVGMFFFLRYFFKLNSVVYKPVGVVLIIL
                     VGIQMMFSELRFGGDPESNTIVGKGPAIAPTAPADTYNVFFGVGENDFTSIPVGSGNT
                     FIETNTFMAKSNIYIRGDVPVRVLIYPTLGVVTLPNGLRFIPFFKNVYKTRAYNDNEK
                     AITIKVNITLGAVNIMER"
     gene            complement(20942..22981)
                     /locus_tag="PLF66_14685"
     CDS             complement(20942..22981)
                     /locus_tag="PLF66_14685"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HMZ65340.1"
                     /translation="MKSIRYKIIFGLLGIQILIFSLLGYTIFDIMQKELVDKLRAYML
                     SVAQACAGAIDPIKHSTFKSHEAKSDPDYIRYFHYLNTIYSREKYISYIYTVNYDPEK
                     DKFFYALDGADAETDKIWLESELFGFSIYNNASNQITVRYDSKEYNSDFSIQTSKGMI
                     NIGISNKPRQKKIFINEIEVIKFISDEPLVITSANKEVHTLNRNLKIQMPIFDQNEIL
                     QITFSKKGEPETEPGTEYVVSETELEKNKRILKSGKDHVDEDTFITNYGKDITATAHI
                     KNEEGVTIGLVILYMESKTFQESKESFLTTAAIIFILTFLGTTLLGFLFANYIANPIS
                     ALEQGVTELAEGNLNTKVILPRKDEFGSLARNFNEMVDNIRTSKEESELLNTQLKNTI
                     ESYSRFVPTEFLNELKRDNILNVKLGDQHTKHMTILFSDIRSFTALSEKMSPEENFQF
                     INGYLGRVSPIIRKHRGFIDKYIGDAVMALFPIQPQDAVLAALEMQKEVFIYNSHRLS
                     IGYEPIQIGVGIHTGSVILGTIGEEKRMEGTVISDVVNTASRLESLTKKFGSAILISD
                     EVYNDLPTLEPYQFRYLGKVRVKGKSKSIKIYELLDAAPTEMIASKIQTREEFHLALK
                     LYQSKGFAEAYRILSEKVFPIGNDKAAEYYIKQIQKFLKFGIPENWDGVEDMEDK"
     gene            23344..25101
                     /locus_tag="PLF66_14690"
     CDS             23344..25101
                     /locus_tag="PLF66_14690"
                     /inference="COORDINATES: protein motif:HMM:NF025038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HMZ65341.1"
                     /translation="MLKKLTKTDVSVDLKDSDFAKLDLKDKEFHPEYFIFQVEALKQI
                     RQALDNPQFYQHFIIAGDEGAGKRSSIISLLQKEYSAKNKFPKHFYFRKTNSIVEDPE
                     YKEGLTPLFDPDAETTPVIYDPTPNTMSLVGIPTEKKYHPGNLIKATGGFLILPISKL
                     VQEPVVYDLLKSCLLTERIDFINLPELNFFQSLDRTLPQFPINVRVILIGDEHIYEQL
                     LKKDNNLHEVFKMKIDLEYEAELNKKNIARFSGLIDSISLKDYPAPALSAKRRLLEEA
                     LKLNESKSKFSLNVTDIKTIYEEAMVLSKSKKDVKGASITDADIDAAISNLEKRDSLP
                     KKKYYEDLKSGVYNVIVSGKRLGRINGLSIFTPYSTHQEYGQVSVISSRALMGSGNFI
                     NIEREVNLSGDVHDKGVFILQSYLKGLFANFSSFAVDISILFEQSHSIVDGDSATVAE
                     LIATLSALSGYEIPSNIAITGSMSQYGEIMPIGAINQKVEAWFNISRLLGSPKDSFIA
                     YIPTANAKDLVLSAEISKAIQDNKFTIYSFSHVTEVVPEILKMPMGKIEKDGKYTPDS
                     ILRRIEDRLEKKKEVERDS"
     gene            25138..26451
                     /locus_tag="PLF66_14695"
     CDS             25138..26451
                     /locus_tag="PLF66_14695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65342.1"
                     /translation="MYGSTIFNTKISILISILSGIGFIAVLALTPIIPQKKKGNALPE
                     EELPLKTPDQKIYSKKEGAKKEESFFSIKAFKKTQEEIHKSIWGNSFSKPNHDELLTT
                     FGHDNQIRPNPSQQTDIYTYYKDEKVSLNSIGDSRSVTNQILEYRYQNKITETNTLLQ
                     NRNTQIRNVEMSYQINSNFSANFKSTQFDIFDDRLKQYPTTMAGISFTSKYVSTGFIA
                     GESSFNNPYRYNQVGFLNGNNYYRDTEIQLRDTDRANRNLFEWQANISPTKNLMFQTA
                     VYNSNRSLINETNVSEGVRIAMAIGLKYIVLNFKYNYLSDNLMRSLMRPDSPSFFNKD
                     YAAFGLTFFIDKNKKYSIYIGNNVYNIATMTKPTDSSSTVPVNNSFSASFRGRNSDFN
                     TTFFFNFKNNANREFYYSNFGVFRLPISSQVNFDYATSMGLELSF"
     gene            26468..27703
                     /locus_tag="PLF66_14700"
     CDS             26468..27703
                     /locus_tag="PLF66_14700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65343.1"
                     /translation="MLISSDLILSKTYQYFHTSKRLKVVKNLTAKLVLYSMTCWLILY
                     CASSDTNRRLITVNPSDLYWRDLSLFLSGLPIDEKNRFYKLTELSSYKSYAKTIDSYW
                     TKIQSDYLDKVRPFKKQYMPDFAKSNTAFYPLSGADFINLNAFYPDAISNIMVGLEPA
                     GKISDPNSLTQDQLKKGLDSIQSMVGEMAAQNYFTRKRMRREFANPHFSGTSPVLLIF
                     LTRLGLTIDFWERVELSANGKLIPETQAHIEDKNPDKVEAVRIYYHMINDKYSRELVF
                     FRMYINENSAEESTSEGKFFLSQGRFNLLLKSAEYVLQMSQYRKFIETLLSRTDIVVQ
                     DDSAIPYNAFSKDIWSPQLFGIYTARARLQNTPNVPDQEDLIKDFKADAKPLPFKYGY
                     GVLKGNDKSNLMILSRKQK"
     gene            complement(27955..29301)
                     /gene="hemN"
                     /locus_tag="PLF66_14705"
     CDS             complement(27955..29301)
                     /gene="hemN"
                     /locus_tag="PLF66_14705"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016545636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HMZ65344.1"
                     /translation="MDRSKLIAKYDIPAPRYTSYPTVPYWSDSPTTEEWLDAIRKSLE
                     PETAAISLYLHIPFCETLCTFCGCNTSITKNHSVEEPYVANLLKEFQMYIDRVPEITK
                     RPIKELHLGGGSPTYLSVDNLKALLTPILSKMNVVKDPSFAIEVDPRRTRKEQLEALR
                     SLGFNRISLGVQDFNPEVQRMINRHQPFEKTEYITNTARELGYSSVNFDLIYGLPKQT
                     SESMASTIEQTLTLKPDRIAFYSYAHVPWIKASQRLFTEADLPKGADKRALYEESRLK
                     FESSNYREIGMDHFALTTDKLWKAYETKTLHRNFMGYTEYKTDIQLGLGVSAISDSWT
                     CFHQNEKILKKYQKRLDAGELPTLRGHKLTKEDLEQRSLILQLATQWNVSLPSSLVEN
                     TKEYLRTMEEDGLISWNQNTLSIEESGRPFLRNICMGLDLRLRTKSPETRVFSQAI"
     gene            complement(29301..30323)
                     /locus_tag="PLF66_14710"
     CDS             complement(29301..30323)
                     /locus_tag="PLF66_14710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016551352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HMZ65345.1"
                     /translation="MASERFTNAINYIEQNIPPVWMMRQAGRYHSHYQNLRSKYSFME
                     LCKIPEVAAEVALGPVQDFDFDLAILFSDLLFPLEGLGMGLKYTDQGPRLDWHLDATN
                     FDKLKSATDAIELLQFQKECVRLTREVIPKNKSVIGFVGGIWTLFTYAVIGKHDGSLT
                     QAKVLTETRKKFFSLMQDILTQNIQLQLDAGAEVVMIFDTAAGELSNSMFNEIVFPYV
                     EYFAKTFPKKIGYYSKNTTDTIVRKVLTQSNLAGVGFDHRLPLTNLIRENKFGFTQGN
                     FDQSLLFLETSEFKKVLKSYINEMKQLSPKERIGWVSGLGHGVLPKTPETNVKLFVET
                     IREEFA"
     gene            complement(30313..>30463)
                     /locus_tag="PLF66_14715"
     CDS             complement(30313..>30463)
                     /locus_tag="PLF66_14715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001201859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HMZ65346.1"
                     /translation="EEKFPSNRTIDNSIVRLRQSLGKHGLKAIKSIRSVGYRWMGEEG
                     EFSGE"
BASE COUNT         9168 a         5257 c         5139 g        10799 t
ORIGIN      
        1 tttattttta aaggctgact tttaattctt taacatttcg caacagactg aaaatatctg
       61 taattaaatc aactctagct gtttcatctt ttacttgtcc ggttgcgttt atctcactgg
      121 ttgaaagctc tgccaggtgc gacgtagcac cttccccatt tactttttca aaagagtcaa
      181 tgactctaat atgatcattc tttttcttca cccaatcagg agtcttatta aatgtttttg
      241 caatttcaat cgttgaaata ccaggcttct tttctctaag cattttaatt gcagtcgcat
      301 aattatgatc tgtccaggct ttacgctctt cgttctcttg aaactggaca gccaatgtat
      361 caatcgagtc gtcgtaatct ctaacaattg catcaacagt tttccatttt agaattttgg
      421 atattgcggt aaatcgtcgt ctacctgcaa ctacttcata accttcttgt tttatttctt
      481 gccctttgga agttatagct ttaagaattc caatctttct taaaacgatt ggctgaatga
      541 gtccaaccgt tttaattgat tcagcaagtt ccgacatttg cggatcattt aattcatatt
      601 cactttcacc cttgattgaa tcggtaaagt taattgatat taggctaaca tcaaactttg
      661 ttttttcata gtttgaaatt ctgattttat tatttccatc ttgaatatat tcctttcttt
      721 ctgctaacat tataaagctc ctattaagtt ttgtttttga gtttccagtt tatcaatttt
      781 ctcttttaat ttcttctctc gaaattctct gtaagattta tgcaatgcat cttgattgat
      841 tccattattg taaaatgcat aatctataaa ttcctctagc tctgtttttt taggcattgg
      901 ctttctccgc ataaacataa atttttcttt tttctgattc aatgaacaaa tcaatttcat
      961 ctaaagccct ttctagttgt tgattttttg caactgacat atttgaattt ataacgtctt
     1021 ctatgtcaga aatatcatct tctataagtt gaactaatct ctcgattcgt gccaaactat
     1081 tactaaaaat cttttctttt ttacttgatt ttgttctgtt agaaattatt ttttcgaagc
     1141 cattttttgt ttttttaaaa ctatcattca gctctgaaaa ataatccttg taatcttgga
     1201 ataaattcat attaataacc cctaccaaat aaaagtaatt gagttgaatt cactggttta
     1261 ggctcattaa cctcaaccgg cttttcttct ttgattccag gcacaaacaa ttgaataaaa
     1321 ttcatgtaga catttctaaa atccacttct cgattttctc cagtgataca atagagagat
     1381 tttcttgaat tgtaaatcaa agccttataa ctataagccg ctccattttt agaaatgttt
     1441 ctttctgcat catcttgaaa taaaagaata ggagtaggct tttcattttc tggaaattga
     1501 taccatgcga aaccttcata agtgacagga tctttaaatt taaaagtata ctcagaatga
     1561 tttcctagtt gtaggatctg catacctgga ctaagatgaa tgtcagcttt gaaatcatct
     1621 tccgagcgag tattaaaaat aaatccgcct tttataatcg tcccattaaa ttctttcttt
     1681 ggaatcaaaa cgatgttagg cgctccattg attaggtgta tcatgattca cctctgtgaa
     1741 atttgaaacc aggaaagagt ttcaaaaaaa gttttactgg atctttaggc ataaaaaata
     1801 taccggcaaa gggagcgtta aacgctccta gaataaatcc ggttgcaagt ttttttaaag
     1861 gcttgtcttc gaattcatta caactagatt caatggtcat ccaattgcct ctagccggtg
     1921 aacttgaatt attgcagaaa acaaaatatg gatctgattt tttcctagtt ccgtgaacgc
     1981 aattcaaaca acaaataatt gttcctactt cattttcatt catatagatt cgccttcttg
     2041 tttgtagtat tcagctattt ctgcttgacg ctcagcttct gctttttgga gatcttcaat
     2101 aaagtcagtc aattcaatgt ttttaaaaaa ttcttctttt gaaatgtaac attttttttc
     2161 atgcatttct cttgagaaaa attcactctc ttcattcata acatatactt tgtagttaat
     2221 tgaaaaaact gtttcaattt tttctggttt gtattttata aactcataga ttttacgctt
     2281 atgttcgtaa ttcaaacttt ccgaaatata tacatctgta attaaagcag attctaaaat
     2341 catagggttt gtttcgttct gaatataaac cttatcgccg actttaaatc ttggttttac
     2401 ttcttgactt ttttcttctt cttgtgttac tgtttccata ttaaacacct ccaagtgttc
     2461 ataaattacg tccagtgctc gaacactgga cgtgtcttat attttataaa acctaattat
     2521 cgttatcctc aaagactact ttgaaagatt ttgtttcctc taactctgag aaatattttt
     2581 ctatcgtttt tttgtctgaa acactcattc catattcccc gatagtgatt atgatttttt
     2641 cattctgaat taaaatcttt gatttaagaa ttctttcgtt tgttactgct gaaaaattct
     2701 tcttagaatg cttttttaaa tctgctataa tatccgcttt gtctttagcc ggtgcgattg
     2761 ctgcttgctg tgtttggaat tttttcaggt gcgacgtagc accggaggtt tttttatttt
     2821 gaaaagggag aaagtaaccg tctttcgttt tttgcaaaat tccttttcct tcgagaaggt
     2881 ttatagagtt ttgaatttcg ttgtcatggt cgttttgtag atttagatag ggttgtaaat
     2941 attcaatcaa aggtgtaggg tgaaataaat aaacgttagt tctatctgta gacaatcgcg
     3001 tatcaatgcc taataactgt ttatctctaa ctgttaaagc tttcttgatt gtgataactc
     3061 cacgagctgc tagagaatct attctcgcct taagcacttc atattttttt gtagcattga
     3121 aaatataacc tgagatttgg cggtaagaat aaattaactc aattgattca gattcttttt
     3181 ttgttagtcc atattgcttg aaaatctgat tttttcgaat tgcttccaaa aaatattttc
     3241 tttggttatg atacattgca acaaagaagc tcaatagaat tgcttccaca aaatcccctt
     3301 cgcaaaagtc aaccatccat tgtgtaatct tgcgatagtt ccctattggg tgaatgtctg
     3361 gatctatgtc aggaactttt gcaaagtcta tcatttttga cctctaagtt ttcgggtgaa
     3421 cattgtaata gttttaatag tcgattccat taaaaaattt tgagttctta gtttaaaata
     3481 atggattccg tcaggatctg gaattttgaa aaattcagtg cgggttaatc cgtatttctc
     3541 acgtatctct gccatctcct tgcgattcat gcggataata aaggatagct cttctatctg
     3601 gtgatttttt tcatcttcta aaactgcgga caattttttt agaattttag tagattctag
     3661 atctactatt tcggtttgat ttgtattcat gcaattaacc tcttacccga tattttctta
     3721 atctctttga taaatttatt ttcgaattta gaaaattgtg cattgtcttt gtttgtgaaa
     3781 agtctatacc atctttctct tgtcgcgtct gtgtgcccga atcgctcgcg catccagtca
     3841 ggagaaattc caggaataga ataatagata tgatttaata aactgattct tagaattttg
     3901 ggagtgacta ttttttttat tccgactctc ttagttgctt ctcttactaa gaattgaaca
     3961 gttgttggtt gtagcggttt attatcctta gactcaaata agtaaacttt accggcatac
     4021 tgttttctaa tcatagaata aagccaaact ggaattgtga ccgctcttct ttttcgattc
     4081 tttccttcta tgatgattgt aagttttgtt tttttaggat ttactttgca gtcagtcagt
     4141 agaatttttc tcaactcgtg gtttctaaat cctgtaatgt aaagaaaaaa gattaaacag
     4201 aattctttct gacttgcata agccatgatc tggactactt cttttaaagt tggcaattgg
     4261 tttaagtcta atacggcagg tctaattctg tattctaatg atctaaaaaa ttctcggaaa
     4321 actgcaacag ttttgtaatc gtgtttatcc tcataatatt cttggattga tttaaaaatt
     4381 gcaaacttat gggcgtgtag attagcttca ctgaatttaa gtgtaaagcg taggtataca
     4441 aaatactgtc taagtatttt aaatgttatc cgcttatatt ttttctgact caaccaatca
     4501 ttaaattttt tgaccttacg attgtattgg tcaatggagg tttgttgtaa atttttcatt
     4561 gattctttca tattcctttt tctgaaagaa tcggtctttt gagttgacag tatattagct
     4621 aaatctactg tttataaggt tagttatatt aaatccaaaa ataatgacta ctgatttgaa
     4681 aaaatcccaa gctaaaataa ctaaaaaata cttcggtcga agtaatctac atgcgtatca
     4741 aaaaaatgaa tcgcagcttt gatttctagg aaatcaaaac tttgtttggg ctagagatta
     4801 gaacaaaaga aaatgaaatt cagataaaaa cgatatccga acgattttac tctacttcga
     4861 tcgaatattt gtaattcgat caaattagtt taccttgatt ttagataacc tgaaagcaaa
     4921 agagagttgg aataaatgtt aggattcata actcactaat gaatgggtaa taattgatta
     4981 ttaatctaat tcctttttca aaacagaata ggttgagatt ataaaatatt tatccagata
     5041 gtagggagat aaattttagg agaaataaaa tgagaaagat aatcttaata ataggaatcg
     5101 gatttttatt gaattgtaat cttttttcaa aaaagaaaga agatgacaat aaattaggaa
     5161 tccttgcata tcttgcaact agaacgagtg caagtgaaga tacaacaaac gatgggattt
     5221 atcaggattt ttctgttaca tccggaacaa ctgtaaatgc cacatacaac gcatctgctt
     5281 tatctgttca agatttgaat ttagcaatca catctggaac agacaataat acgaaagtag
     5341 aagtttctgt agtagatcca gatggattgg aacagtctta cttccgaaat ggagatggag
     5401 caatgacagt taattttact cctactaaga cagggactta tagagtaaat tttaaaaaca
     5461 actcagaaag aagcgttagt ataaataata gttctgtttc tggtggaacg gctacttcct
     5521 ctgcaggaag atcgaataca aatgctaaat ttaaagatat attcttaaaa gcatatgttg
     5581 gactcagcac aagatgtaat acttttaaca cacaagttac agctgcaagt ggaagctttt
     5641 atgtgcaacc gattgtcttt ctaggtaaaa tcggaactgg gaaaaaagtt tcggatatta
     5701 ctactgccac aataaagtta gcttatggtg gaactactct aacattgcaa agattagatg
     5761 atatgaatgc aaatctttat gataatccaa acaatccatc aaacagcatt ccatcatctg
     5821 ataaagcgaa taaaattaaa tatataaaat cttattatca aagtctattt ggtgcagctg
     5881 gagaattata tacaataggc gacacattta ataaaagcac aggatctact tgtagtagtg
     5941 ctagcacttt accacttgga acttctaact ttggaaatag tgttataact atgacattgg
     6001 ttgattctgc aaatggtata aatgaatctt ttaaaataag acctgttgta aatgcaattg
     6061 attttagtca gttttttaaa aaatcagatg gaacagcatt taatgatttt agcacttgtt
     6121 cttatgataa aacgacaggt agttctgctt cttgcgcaaa gacatttagt ttaacagatc
     6181 ctccgtatgt tcaattgtct tcccaggctc ttgatacaag cgttagtgca ccaacaagac
     6241 ttttattttt tggagcaaat tatcctaagt cttactatac tcaactcatt tcgaattcag
     6301 ttgccttgag taatggatca atgaaatcta taacaattga tggttgttta aacaatggag
     6361 gattgaatag cgttaaatta tcagcaacgg ataaagtata tttgccgctt agagagttta
     6421 atttagcgaa agacgatatt agccaattaa aatttcaacc agggacttat actcacttaa
     6481 aagattttta ccaaggaaat gctgatcttt ctggtagtgt agatttgcca gtatgtattc
     6541 cctcaactac acaagaatgt ccaacctata aaacaattac agtgaattcc tctaagtgta
     6601 gagtgaaagt tgactctggt ataaccgttg gagcctttag ttcttctcca acctggacta
     6661 ccgcagacac aaattatctt ttatatccag ataatctagg aggtggactt agcgctcgta
     6721 ttactgaata aggttaaata ttcacttaga tggaatgtgt agagagataa gtcagttttt
     6781 tggatctaca catctccatc aattttttaa aaaaacattt gcaatagaaa acgattcttt
     6841 gttacatttg tatttgtaag gagagctatt atgaatgcaa ttaagaatac aacattgcta
     6901 gaggcagtcg cggctgtaat ccaacatgaa aaggattgtt ttgattttta tctagacata
     6961 tatgagaaag tgaaagaagg gtctgtaaaa gattttttca aagagctagc agaggatgtt
     7021 gaagaacata tcaaactcat tcaagaaatg tataatgatt taagtggagg agtagagttt
     7081 ccaaatctaa agcaactcac tgcgattcat aaatttgata aaactcatct tacaaagcta
     7141 atgagaaaga ttgatcgtaa cactgaacat gcatttaaag atgatattga tgctgtgcag
     7201 agagcgatgc aagtttcaga agatgcaaga gaattcgctt caaaaatgaa agaaaaattc
     7261 aaagatcctg cgatcaaaag tttctttaat aaactggcat ttataaaaga agaagaacgt
     7321 attatggcag aatctcaata tctattctta caacaaacgg acaaaaccaa atactattgg
     7381 gaagacgatt cgcttgtgaa tagtaaatag gcaaatattg attcgtttta aatgaatcac
     7441 ctttcgctat cattgcttaa taaaagcaat gattgagttt gggtgaacca aacatctaca
     7501 tgaggtttgc ttctagagga ggttttaaaa tcgcacaagc ttttgatttt atgcattagc
     7561 attctctaat attttaaaat catcatttat tcctcaaatg atcaattttg ggaaacctca
     7621 gttaaataaa ttattttctt tcaaaaatct tcatccctga ttatgatttc ggggatgatg
     7681 tttattatcc gctagccttg acttttttta tattctgtaa gttattcatt gccaaataaa
     7741 gtatatttaa ttttacatgt tacttttttg aggtatattt cagcaaggga tacttactgt
     7801 taatctacca agattttaaa atacttgaat caaacaattt tgaggtaaga aggaatgtcg
     7861 aatcgattta tgttcaataa aacgatctat tttgtttttg gtatttttac caacttggga
     7921 gaaatatatc ttttcctttt tctttttact caaattacta gctgcaattt tatatctgct
     7981 tcaaaagcaa aaacgaattt agatcgaaac cttttggtag ctggggttgc cattttttca
     8041 aatcaatcta aggatgatgt tgtgcaatac gctcaaacct gtaagcagga acttgggcta
     8101 agtgctgaaa ctcttgctcc ttggaattgc attgatggtt tagaaattcc aatcacagta
     8161 aatgggaaaa ttccagatga tgatacttat cgaaatttaa tgcaaggaaa tatagcttgt
     8221 gattatccat cttggctaca acctcctgaa gcttgtttga attatgcttt tgttcaggaa
     8281 agaaatctaa gcccagatgt gaaggcagtt ttactttgtc gtgctcgtga ttctaatttg
     8341 aataaaactc gcgcacaacg tcttttggat ctaaagaata accctacaat agaaaccttt
     8401 ttgaattatt atagacaacg cacaattgca ttaatttgga caaatacaaa gaccggcaaa
     8461 acttgttttt ttgattctag agtggaatat gcaggctata ttccgtcacc cgatgacact
     8521 cgatatccga cttatgaaga ccttcctgaa ccgaagtttt taattgatgc aaatggagaa
     8581 gcgttagata aaagcttatg gcaacgtaat gcctattcgg tgtggaaaaa tccttccttt
     8641 gtatttaata cagatcaatg tatatcctgc catacagcaa gtccgtttat tacaagtcct
     8701 tggttaaaac aagtctatca acctccgcgg ctatcttcgt ctactcctta tgtaattatt
     8761 ggagatttct ataataaaaa atcatttccc ctctattccg tgacaacgga accgatttac
     8821 aaagatggat tactagaagc acaagtttgc acttcttgtc atagaattag caatcgtttt
     8881 gaatgtgaca aactgatcga ttattctgtt ggcttaaaat caccagtgtc aaagccagag
     8941 aatgcagttc ttccaaatct ttggatgcct ccagaagtgc agaacacagt aactacaaaa
     9001 gaggattggg caaggaaata taagtcacac tatgatcgac ttaaatgttg ctgccaaaat
     9061 ccgaatgcta tcaattgttc ctatcaagat ataaatcaaa attcactatc cacacttcaa
     9121 agaggaaaag gtccagaggt ttgtaattaa atggaaccga tttcaatgtg gaagattctg
     9181 attatccgct acgtaatttt ctatttaaaa taagaattga tacaagtata actaaattta
     9241 agttggaaaa gtctggatgc attttgacca gaaaattata agtctttttc ttttatttgg
     9301 atgaatagca tgaacgattg gaataaaata tacaatgaat acccagtaaa ccaggaactt
     9361 atctggttaa ataactgcgg aacaactcca gcaggaaata gagcaattaa agacgttact
     9421 cgttatttag aagcttattc tagaatggga gtgtttaatg agacggaaaa atatccaacc
     9481 gttaaacgac gcattacaga aatacttacc tccattttaa attctgattc tgaggagata
     9541 ggaatcatcc acaatacatc cgagggaatg aattttattt cccatggttt aaatttgcaa
     9601 tcaggagatg aaattcttct cttagaaaat gaatatccaa gcaatgtata tccatttcag
     9661 cactgggagg aaaaaggagt gagtttgaaa tttactccaa tggcaaatac tccaaaggaa
     9721 tttttagaaa attttaaatc ctctctatct cccaaaacaa aagtcgtttc tctttctctc
     9781 gtgcattggt gcactggtat gccacttccg attgaagaaa ttggaaacat ttgcagagag
     9841 agaaatattg aattagtaat tgatggagcg cagggagtag ggttgattcc tgttgacgta
     9901 aagaaaatga atatttccta catggctttt ccagcatgga agtggctctt aggtccattg
     9961 ggacttggtg gaatatttgt tgcaaaagat cgattagcca cgatgaagac aattttcaaa
    10021 ggacaaaatt ctgtagtgaa tgcagatgaa tatctacctt ataaaaatca aattcgctct
    10081 ggcgcagaac gtttcgaata ttctacaagt aattttacag attgggtgta ttggaagtct
    10141 acattagaaa tgctttttga gattggtttt tccaatgtcc aagagagaat ttactcttta
    10201 cagtcctatc ttgcagatgg ccttagaaag aaaggctata caatttattc tgattcctat
    10261 ccggaagtaa aaacaggaat ccttgcttgc gagaaagctg ggtctgattc agcaagtctt
    10321 gtcaagcatt tgagagagaa taaagtcatc tctgctgtgc ggttaaatcg tgttagactc
    10381 gctcctcata tctacaactc ctttgagcaa ttagataaag tcatagagct tttgtagaga
    10441 aattacgcag ggacaagacc taattctttt atgctatgaa tggcttttag ttcttgcttg
    10501 tttttttttg tgctatcaac gtcagacgca ttcacaaatg tccatttaaa aaaatttttt
    10561 tctttctaat ttaattgtaa gaatgaaatg cctagctatt cagggtttta tttctttcta
    10621 gaaacattct tctttttagg gctctgcaat tttttgttct ttaaagtata ttgattccaa
    10681 tattctaaaa gttctttctc tgataaccct tcggtttgct tagaaatttt ttcctgagct
    10741 tttcgtttca ttgcaattgt atcaaacttt ttagttttca tattggataa cctctggtga
    10801 tctcgtaggt tacaaagttt tacttatctc gttcatgaga tcaggtattt catttttaac
    10861 aacaaggtaa actcttttat aatctacgcc aaaatactga tggattgaaa taattctcca
    10921 atctttccac gcttgccaag gaatagaagg atttgcttct ttaaattctt ctgataaact
    10981 tccactagcc tctcaaataa tttcgagtag gcgaaccgtt gcatgttggt ataaacgatg
    11041 cgataaaaaa gtttccacat cagaaaagtt cgaaataatt cctgttaatt ccaaacaggc
    11101 atctagtata tgactcaggc gaacactgtc cctatcgttc ataaaccaaa ctcatatctt
    11161 tctggataaa tggatttctg aaattgattc cggtgttaat agatcaactt tttgcttaat
    11221 ttgtttttct ataaaattct actctcttaa taaaaaggat ttatcatatt ccaagtattt
    11281 tagtatattt tcatcatctg aaattaatgt atactttgac tcgatagctt ggcagattag
    11341 aattctgtca aatgggtctt tatgaaattc aggtaatttc tcaagatgtc taaataaact
    11401 gttatcaatt gaacactaac taaaactgtg aatatttatt tgagaaatga tgtaatcata
    11461 ataaggtgcg ggtaatggta attttcccat cttggcttta acgatgattt cccataaaga
    11521 tgccgcactt atgaaaattg tattttcaaa atcttctata atttttcgat actcaacttt
    11581 aagtttttca tttccattta tataccaaag gaaaatatgt gtgtccaata agaaattcat
    11641 aatgccccaa atccaatcaa aatatcattt ggtaatggat caaaaaagga atcaggaatt
    11701 tgaaagtcat tcttcgccaa accaaaaggt cttttttgta actttttctc atctgattgt
    11761 tctagaggcc aaataataat ttctgccttt ttatttttta attcctttgg aatttctaaa
    11821 atgtccggta aatttgaata tattattcta attggttgca tcacaaactc cttttccata
    11881 tgttattcgt ccattgccaa aatgcaagca caatctttag gcaactctcc atcactcaca
    11941 cctctaatct cggcaacgtc ttcacaatct tcatcgtctc gaggctaacc gtaattatcc
    12001 tctgaaataa ctccaacgga tactttggat tcttctctgt ttcgattccc cagaggttta
    12061 catgtctgtg atcccactgt ctttgtcggt ctttacacaa taacgttcta cgatccagtc
    12121 taatgccggt ttgccgttta cgatgtattc gtaggcttct tcggggatat cggtgattgt
    12181 gataaaatcg ttgtattgta aggaacgcat atatgcgttc cctacgtcgg cggtgtcggt
    12241 tgatttcata tatttcatct gggttacaaa atatttttct gcctcgaaga ttttttcgta
    12301 tttggatttt gtcgttgtag tggacgcacc gccgtgcgtc cctacgattt ttacggattc
    12361 gttgatcgta actggataca attccgcctt ttcgtaatcg acgtgaatct ttgctaattc
    12421 gcgtcctgct tttgtgaacg ctcggaaatc ttctacccgt ttcacgcaag gaatccgcgg
    12481 cagttctttc gataaattgt ccgcatactt tgtcttatac tctggactgt gcaatagtcc
    12541 gtagatataa taaaaaatat cttctttcgt gattttttct tttggatacg cggtttggaa
    12601 atgcgctaga cccgcgtcgg ttatcgcatg acgtcgccgt agggacgaac ggccgttcgt
    12661 ccctacgggg gatttaaaca atccttcctc cgccaaatct tccttatcct ctgcatcggt
    12721 atcatacaaa tacaacggaa aacattggga cttggaaatg atttccaaat ctggaatgat
    12781 attactaatc atcgtagaaa attcttttgt agaaccaata ccagtcacgc aaatcaccaa
    12841 attttctgat tcgggtgtgg gaaagatttt ggggatttgt aaaacccttt caattaaatc
    12901 tctattgaaa tataaatttt gctttgtaaa aggtctatac attccgtgcg tgaatgattc
    12961 attatataaa tttaaaattt tattcttttc taatgatttt ttcaaattta cagtccaact
    13021 tattttcttt gaatcagagt ttataaatct atcagtggct ggaaaatcgc cttccttttt
    13081 ccctttacat gcttttttat atctgtcaac ttctgaatta taaaaagtaa tcatcctatt
    13141 tatattttca gttaactctt tcttagaaaa attataaacc caagaatccc tatttgtggc
    13201 taagcctaaa gaaaaattct caaacagcac aacactcgac ttatccttct tatctccgat
    13261 ggaaatgaat tcgtagaaac tatcgtctct ttggttaatc cagtcgttgt atttatctgg
    13321 gtggattttt acccatgcct tttgctggct aataccgttt atgctcttaa aggtagaaat
    13381 ttttcccaat ttttcttgtt gggttagata atcgcctatg tcatgaaagt aaatttctcc
    13441 ttgtttcttt gcttttgcgt tttttacaaa gatgctgatg gcgataggag ttcttgttcc
    13501 ttgtccgaat acattgtctt tttcttttct tctcaattcg cctgannnnn nnnnnnnnnn
    13561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13621 nnnnnnnnnn nnnnnnnnnn nnnnnttgga aactatcagt aagaaggatt ccggtaaacg
    13681 gggtgtaatc ggtgtttacc aaatcatgat atgttgattc aatattaatc gcggcgatgt
    13741 aataggcgag taatacgatt tcatttgcat gaagtccggt tttgtatttt tggatgagtt
    13801 cttcttttgt gagaagtccc gattgcatga ggcgggtgat gaaggttcca gtgcctgtaa
    13861 atggatctag gatttggaca tttttacttc ccagactttc gccaaactcg gagcgcaaaa
    13921 tatcgtttac actgtggata ataaaatcca ctacttcgac gggagtataa acaattccaa
    13981 gtctttctgt gagttttgga aatgcatttc ggaaaaattt atcatagagt tctaaaatga
    14041 ttttttgtcg tccttgtgca ttttcaattc cttctgcacg cattcgaacc gatgcataaa
    14101 aactcttcag tgtatccgct tctttttcta tatgatgctt ttctaatata cttaaaattt
    14161 catccatcgc acgggagact gagttttgtt tggtaaattc atatttgcta aagagtgcgt
    14221 caaagactgg ttttgtaatt aagtgttggg cgagcatttc aatgacttct tcattggtga
    14281 tactttcatt caaatcatct cggagttcat ccgcgaaatc gtaaaatgcc tttcgttctt
    14341 ttgtattgtt tttattttct actatcgttc gtattcttgt gatatgagtg tttgcaatct
    14401 ttgcaatatc atttgcccaa tcttcccagt ggtgacgatt cccacatttc tgaactactt
    14461 tggcatagag agcaatttct atttctccaa tgtcgaattg gagtttttgt tgggtgtcgg
    14521 tttttgtagg ggttggtcgt gaccctcccg ttttgccgat agtattccca ttaccagatt
    14581 ctcccgattt accaccggat tttgttttct ttgcaatgga atctgtgact gcaatcactt
    14641 ccattttgct tttatctggt ccgattaatt ccattttgtt aatcatcgca tcgaatctat
    14701 catcatgaga tctgagtgct tgcaaaacct gccagactac tttgtaggtt ttattgtcgt
    14761 ttaacgcttc atgcgcaggg attccagcag gaatcacaac gggtaatatg acgtaaccct
    14821 tactcttgcc ttctgctttt cgcatcactc taccaacgga ttgaactaca tcgacttgcg
    14881 aatttctagg agttagaaat aaaactgcat cgagtgctgg aacatctact ccttccgaaa
    14941 gacatcttac attgcttaga atcttacata cattttcttc tgaatctctt tttaaccaat
    15001 gaatctttga ttctttttca gttgcattca tacttccatc tacatgttct acttggcatt
    15061 ttaaattgat tgtttcattt gtattatttg agaccggtct cgaccgttca ctactgatgt
    15121 aggaatctac aacagcttga aacattttag caatttgttt agaacttact ttatgtgtct
    15181 ttgaattctg gttgtattcg atcacttggc aaaaggctac cgcacgacgc atcggattat
    15241 cttcctctaa atcttctgct ccttgtttag aaagcgcctt ccaacatcca atgatttttg
    15301 ccgcatcgtc tactttgatt tggttgtctt cgtcagcgag taatttttgc aaccgactac
    15361 taatgtgtgc ttcatctact gcaagaacaa ttactttata atctacaagt aaatcacttt
    15421 gcactgcttg ggaaaatgtt attacatgaa gttgttttcc atataacttt tcatcatcca
    15481 ttgagcagag tgctacattt tctttttctg ctgtgacttt agccgcttct ccgtaaattc
    15541 taggagttgc agtcatgtag aggcgtttgt ttgcacgaat gaattttcca tcgtggactt
    15601 ttacaaattt actttcgtct tcgtctccga aggttgcgcc tgtcgttcta tgcgcttcat
    15661 cacaaacgat taaatcaaag tcttccaatt tgtatttttt ttgagcctca ccaattacat
    15721 caatcgaatg ataagtagaa aatacaacac tcatgtgatg tgcatcgtgt ctagatttca
    15781 tttcctttgc tagtttactt ggctctgtag tggcaggaaa ttgtaattca tgggcaaatg
    15841 tatttacaac atcatcttct ttgtttctct tttttccaac ttcgctatcc gaacaaaccg
    15901 caaaactatg aagtggaact tcactttcct gtgtccactc cgttagcgac tgcgaaagaa
    15961 gagctagact cggaactaaa aacaagactc gtttgccctt acccgctaac ttctctgcaa
    16021 tcttcaagct agtaaatgtt tttccggttc cgcaagccat gatgagtttc ccacgctctt
    16081 ctttttttaa accttctaaa actgatttta cagcaatctc ttggtgaggt cttaattttt
    16141 ttttgggttt aaaaactggt ttggttttgg atttatactt cgaccaatca atttggctat
    16201 tctcaagtgc atagagatca attttggtaa ctggtggagt ttgatttaac aaagcatctt
    16261 ctgcgtgttc actccaattg tttgtagttg ttacaatcac tctgtggcta aatggctttt
    16321 tccccgaagc agtaaaaaag ctatctatgt ccgacttttg cactctatag tctggatcgt
    16381 agaatttaca ttgaatcgca taatactcgt tagtcccatt tgcccgcgcc accaaatcaa
    16441 tcccagtatc tcgtgcatcc attccttctt gtttcgccca atcgccataa tgccagactt
    16501 cttcatacaa gtctttgtat ttcggttcgt tttttaaata cgtgcgaatc agttcttcaa
    16561 aataagttcc cttctcacgc tccgaaacag caacagtgcg aaagttgtaa agcaaaactt
    16621 ctaagtcaga ctttttggct actttgtcta acgttatgtt ttcttgtttg aaaagagatt
    16681 gattgtttgt catttacaca tactccatat agacccatgc ccgatcttct aagcatttta
    16741 taaattcata aaatttctta atgcgatatt gtaagttctc taaacttcct tcgaaagagc
    16801 ttgctttgag tagccccagt ttagaactag attccatttt tacaattaaa tcatagctct
    16861 ctcttagaat acatttttga aaaaaagaaa tatacgaatt gcggttttgt tctgttagct
    16921 cgactgcttc aatttcttcc gcttcgtctc cttcaatgtc cactttataa actttggaag
    16981 tttgcatttt ggaaaattca gaaagtgcta accatttata aaaggattca gatacctcta
    17041 agatatattg ttttccattc ttagcataaa aaataatttc cattgttaaa tctccatact
    17101 ctttacattt ataaactcgc tctcaggaat attctccgcc caagctattc cagcttcgtc
    17161 aaatagtttt ttagcattcg gagtaattgt tccagtagta actacagcag ttatagttcc
    17221 tttttcaatt aactcgaatg ctaatcctat attgattttt tctctagttt taatttctat
    17281 atagggcatt attttgtatt atatttttta tttaagtcac gaactatttc ttttactttt
    17341 tctgtatctt tctcatctgg ataataaata tcacctttgg gatcgttttt atatgttctt
    17401 tctgattgga tattttcttt ttgaaaaaga gtaagcatcc agcctatcag atttttatgt
    17461 tctgttagct tctgaatcat cctatcttcg tcggttggct tatttggctt ttccattttc
    17521 agattacact ctaaaagtaa atgatgttaa aacagaaaac tctagtcacc aagcaaggtc
    17581 aaatgttttt tagatttttg gcaaacttag tgagtaggtt ttccctctat ataagaattt
    17641 gctaaacatc aagctattgc tatattaaca caaaaggatt gagagattaa tgccaattcc
    17701 aatcgaaatc accttcctga cgtcaggaaa gtgatctgca atttcctctc tcgaaactat
    17761 ttttccttct taaacatagt taaggctact ttgtattcgt cgttaaatgg gagttgaagt
    17821 tgggataaca tttcaggaag agcaagtttc cagcttacat aataataggc taatatcttg
    17881 taaccggaaa attgtgtatt agggatagaa gatagagtat aatcattctt atccgttctg
    17941 aatcctttgg caccgagtaa agcaatttcg actgcgattt ttttgatatt gtcctgcgac
    18001 attttttcaa aaaactgcaa agcatccacc atatacgtca cgactgccat attcgttcca
    18061 atgatttctt gggcgcgttg aaattgaaac atttccactt ctgcgtttgg aagttctctt
    18121 tctagcgaat tatcgatatt ttccgctcta taaatatctt cactgacaag actgaagtat
    18181 ttatctagtt gtaaggctat cgcccaacgt ttaactaatt catattcgat tgcaggttgt
    18241 ttgtttaatt tcaaatttca gtattcagat aaaaagttct ttgcttgttc tagttcggcg
    18301 ttggttggtt taaattgtgc aattaaatca aatccgaata aatctttata atgaagagct
    18361 gttactagat tgaaaatttt acttttagaa ataagatcag tcggcgcaac ttctaaagtc
    18421 tttggatctg ttactgctcg tattccttct tgtataaaag tataaataga taaaaattga
    18481 taacttctaa gttctggata ctcactgtat atcaaatctt cgataaataa atctatcggt
    18541 gtattgtaaa tctgttgatt gattccttca aataaagcct gagtgtaatt ttgaatagcc
    18601 tcttgttgaa tttctttttt agttagtaag tcaatcgtag actggataga ggaaataaat
    18661 tcttttttgt tttcagagtt agaaataaaa agtagattgg ctttttcttt tcgtgcttga
    18721 atcacaaaat ctaaatgaat gagttcatgt aaaatcaaat gttgaatggt aggataagaa
    18781 ggcttatagc ggagtatatg tttttttcga ttatgatttt ccgcaaattc aatcttagcc
    18841 gcagtagaaa tcgttgagtc ttccactaat tcaatttgtt tatcacattc aaattcaagt
    18901 ttatggagat agttttttat gattgcggtc tctttgtttt cctgaactat tctttctgaa
    18961 acttcaaatg cttgctttaa agaaatttga tatatttgat cttgtggctt acatagtttt
    19021 gccgctgcga tggaatacat aaaagcagtt tgtagattat tttctttttc tgcaataatt
    19081 cctagtgcat attgagtatt tggatactct ttgttaatct ctagagagtg taggaagtat
    19141 ttctttgcct cagaaaattt accctgttgc attagacttc caccgatatt tgtgattaca
    19201 atataatcat tcggattgac ctttagagat tggtcgtagt atttcattgc agtggaaata
    19261 tcattcttaa acttagaaaa aatatttccc atgagcagta atgcccaacc gttctttgag
    19321 ttccatcgca aggagtctat taggcaatta attgcttcgt cttggtttcc ttgttctgaa
    19381 taaacttgac cgagaatgcg atagtattcc gagttagttg gatttttttt gatgagtgat
    19441 tttaagattg gttcggcttc tctgaatttg ccggtatcgc agtattgaat cgcttttttg
    19501 taatcgaatt cttgggattg aatttcagag atgtcaatgt ctattataac ataaccgttt
    19561 tctattctaa cttttggttt gtattgtccg taggagtagt attgctctaa tttctcgaca
    19621 agtagactat tcttatcatc cccctctttt tttatattgg gaaataaatt aaataagaaa
    19681 tcatctattt tatgtattat ttgcattgct taatttcttt gatggatata actttgggtc
    19741 aattaaaaat cgaatatcgc agagaaaaaa catagaaagt gataattgat tttccgtttg
    19801 gcttaatttt tctcgggctt ttactcgttc aaattctaaa atcctttttt caacaagttc
    19861 tgctcttctc gtttgtattg caaagtagtt ttgtgcgaat gcaatttctt cttttctgct
    19921 atcaccattt tgagcaatca gatagcacgc ataacgtgtg agtagtatat caacaatttc
    19981 atgttgagca ccttttccgg cttcgatcac cttcctgaca tcaggaaagt gatcggcaat
    20041 ttcttcccct gcatgagcgc aggcttcttt ggcttttgag attacttttt caaagttttc
    20101 ccatttggta tagcctaatc attcttgtag atcacgggca ctccagcatt caattccttc
    20161 taaatcacta gcaaccgatt caaataaacg aaatagattt ttgatttctt ctgttttcat
    20221 ttcaacctcg atcttctgtc aaatctacct aagctcaatc acaatactca agaattcctt
    20281 taattgtaaa tctttttagg agttagaatg gaaaaacggg ctgtgaaacg acttccattc
    20341 gaattttcaa catctataga aaggaatcgc cgatactaaa aactaggcag gagaactatt
    20401 actatgaatt tatcaatcgc gccaaccata ctgggcattc tactttttgg cgttggaatg
    20461 tttttttttc tgcgctattt ttttaaatta aactcggttg tttacaaacc agttggtgtt
    20521 gtgctcatca tactcgtcgg aattcagatg atgtttagcg aacttcggtt tgggggagac
    20581 cctgagagca atacgattgt gggaaaaggt ccagcgatcg caccgaccgc accagcggat
    20641 acttataatg tattttttgg agttggggaa aatgatttta cttcgattcc ggtggggagt
    20701 ggaaatacat ttattgagac aaatactttt atggcgaaga gtaatatcta tattagaggc
    20761 gatgttcctg ttcgagtttt gatttatcct acgctcggag tagtgactct tccaaatgga
    20821 cttcgtttta ttcctttttt taaaaatgtg tataagacaa gagcctataa tgacaatgaa
    20881 aaggcgataa ccattaaggt aaatattacg ttaggcgctg tgaacattat ggagagataa
    20941 tttatttatc ctccatatct tcaacaccgt cccagttttc gggaatacca aattttaaaa
    21001 acttttgaat ttgcttgata tagtattcag ctgctttgtc atttccaatc ggaaatactt
    21061 tttcagaaag aattctatat gcctctgcaa atcctttaga ctgatatagt ttgagtgcaa
    21121 gatggaattc ttctctagtt tgaattttac ttgcaatcat ttctgtcgga gctgcgtcta
    21181 gaagttcata aattttgatg gattttgatt ttccttttac cctgacttta cctaaatatc
    21241 taaattggta aggttctaac gtgggcaaat cgttgtaaac ctcatccgat atcagaatcg
    21301 cagagccaaa ttttttcgta agactctcta gccgagaagc agtatttaca acatctgaaa
    21361 tcactgttcc ttccattctt ttttcttcgc caattgttcc tagaattaca gaacctgtat
    21421 gaattccgac tccaatttga attggttcat aaccgatcga gaggcgatgg gaattataaa
    21481 taaatacttc tttttgcatt tcaagggcag caagaacagc gtcttgtggt tgaatcggaa
    21541 aaagagccat cacagcgtct ccaatgtatt tgtctataaa gcctctatgt ttgcggataa
    21601 ttgggcttac ccgcccaagg tatccattga taaattgaaa gttttcttcg ggagacattt
    21661 tctctgaaag tgcagtaaat gatcgtatat ctgagaaaag aatcgtcatg tgctttgtat
    21721 gttgatcacc taattttaca tttagaatat tgtccctttt taattcgttt agaaattcgg
    21781 ttggaacaaa acggctatag gattcgattg tgttttttaa ttgtgtattg agtaattcgg
    21841 attcttcttt ggaggttcta atattatcta ccatttcatt gaaatttctt gcaagacttc
    21901 caaattcatc ctttctggga agaattactt ttgtgtttag atttccttct gctaattcgg
    21961 tgactccttg ttctaaagca gagattgggt ttgcgatata atttgcaaat aagaatccga
    22021 gtagggtagt tcctaaaaac gttagtataa atataatggc tgctgttgtt aaaaatgatt
    22081 ccttcgattc ttgaaaagtt ttggattcca tataaagaat tacaagtcca atggttactc
    22141 cttcttcgtt tttgatatgt gcagttgctg taatatcctt tccataattg gttataaatg
    22201 tatcctcatc tacatggtct ttcccgcttt tcaaaattct tttattcttt tctagttctg
    22261 tttcggaaac cacatactct gttcctggtt ctgtttccgg ttctcctttt ttactgaatg
    22321 tgatttgtaa aatttcattt tgatcaaaaa tgggcatttg aatttttaaa tttctattta
    22381 gtgtatgcac ctccttgttt gcagatgtaa ttacaagagg ttcatcggaa atgaatttga
    22441 ttacctctat ttcattgata aatatttttt tttgtctagg tttattactg attccaatgt
    22501 taatcattcc tttggaagtt tgaattgaaa aatcagagtt gtattctttt gaatcgtatc
    22561 tcacagtgat ttgattggat gcgttattgt agatcgaaaa accaaatagc tcagattcta
    22621 accagatttt atctgtttct gcatctgctc catctagagc ataaaaaaat ttatccttct
    22681 ctggatcata attcactgtg tagatatagc ttatgtattt ctctctgctg taaattgtgt
    22741 tgaggtaatg aaaatagcga atatagtcag ggtcagattt tgcctcatga gatttaaaag
    22801 tggaatgttt aattggatcg attgcaccgg cgcaggcttg ggcaacagaa agcatgtagg
    22861 ctcttagctt atcaaccaat tccttttgca tgatgtcaaa aatggtatat ccaaggaggc
    22921 taaatataag gatttgaatt cctagtaacc caaaaatgat tttataacgt atacttttca
    22981 aatatcttac caattacaaa agttgtaaat aataccacca atgaacgtgg atagcttcct
    23041 ttgtgtagat aaaactaatc gaagattcaa tactattagg atattcattg gtttttatcg
    23101 tttttaggtt cgatggtaat tcgtgatttg acgaaatgaa tcattgtcca tttcattgaa
    23161 tcgactaaat tgcaaaagaa actttttcgt cttttcaaaa aggaatttcc atttagatcc
    23221 ttttgtttgc tgatcctgaa gactcttaat tatgtctaat aatacccaat ctttcccgta
    23281 aattctcgga ttgtcaatat taatctaatt gcctgaatag actctattta aaaaatagca
    23341 gatatgttaa aaaaattgac aaaaactgat gtatcagtgg atttaaaaga ctctgatttt
    23401 gcgaaattag atttaaaaga caaagaattt caccctgaat actttatatt tcaagtagaa
    23461 gcgttgaagc aaattcgaca agcccttgat aatcctcagt tttatcaaca ttttatcatt
    23521 gccggtgatg agggagcagg aaagcgaagt agcatcatct cccttttgca gaaagaatat
    23581 tctgcaaaaa acaaattccc aaagcatttc tattttagaa agacaaactc gattgtggaa
    23641 gacccagaat acaaagaggg tttgactcca ctttttgatc ctgatgcaga gactacaccc
    23701 gttatctatg atcctacacc aaatacaatg agtctcgtcg gaattccgac agaaaaaaaa
    23761 taccatccag gtaatttgat taaagcgact ggtggttttt taattttacc aatttcaaag
    23821 ttagtgcaag aaccggttgt ctatgattta ttaaaatcct gtttacttac agaaagaatt
    23881 gattttatca atttaccaga gttgaatttc tttcaatctt tggatagaac tcttcctcaa
    23941 tttccaatca atgtaagagt gatcttaatt ggtgatgagc atatttacga gcaacttcta
    24001 aagaaagata ataatctaca tgaagttttc aaaatgaaaa ttgatttgga atatgaagca
    24061 gaattaaata agaaaaacat tgcgcgtttc tcaggactca ttgactctat tagtctcaaa
    24121 gattatcctg cgcctgcgtt atccgcaaag agacgactcc tagaagaagc tttaaagtta
    24181 aacgagagta aatcaaagtt ctctttaaat gtgactgata tcaaaactat ctacgaagag
    24241 gcaatggtcc tttctaaatc taagaaagat gtaaaaggtg cttctattac agatgcagat
    24301 attgatgcag caatttctaa tttagaaaag cgagattcac ttccaaaaaa gaaatactat
    24361 gaagacttaa aaagtggcgt ttacaatgta atcgtcagtg gaaagagact agggcgaatt
    24421 aacggtttat ctatttttac tccttattct acacaccaag agtatgggca agtgagtgtt
    24481 atatcatcgc gtgctctaat ggggagcgga aattttatta atattgaaag agaagtaaat
    24541 ttatcaggtg atgtgcatga taaaggtgtt tttattttac aatcctacct aaaaggactc
    24601 tttgcaaatt ttagttcttt tgcagtggat atctcaatct tatttgaaca aagtcattct
    24661 attgtagacg gtgactctgc tactgttgct gagttgattg ctacgttatc cgctttatca
    24721 ggatatgaga ttcctagcaa tatagcgatt actggttcaa tgtctcagta tggagaaata
    24781 atgccgatag gtgcaatcaa ccaaaaagta gaagcatggt ttaatatctc acgccttcta
    24841 ggtagtccga aagattcatt tatcgcatac attccaactg ctaatgcaaa ggatttggtt
    24901 ttatctgctg aaattagcaa agcaattcaa gacaataaat ttactattta ttccttttcg
    24961 catgtgactg aagttgttcc tgaaattttg aaaatgccta tgggcaaaat tgaaaaagat
    25021 ggtaagtata ccccagacag cattttaaga agaattgaag atagacttga aaagaaaaaa
    25081 gaagtcgaaa gagattcatg aaaggtcgta gattaaatag gtattcttgc caaagtcatg
    25141 tatgggtcta caatatttaa tacaaagatt tcaatcctca tttcaatact atcggggatt
    25201 ggattcattg ctgttctagc cctcactcct atcattcctc aaaaaaagaa agggaatgct
    25261 ttgccggagg aagagcttcc tcttaaaacg cccgatcaaa aaatttattc aaagaaagag
    25321 ggtgcaaaaa aagaagagag ttttttctct atcaaagcat tcaagaaaac acaagaagag
    25381 attcataaat caatttgggg aaacagtttt agcaaaccta atcacgatga attactgaca
    25441 acttttgggc atgacaatca aattcgtcca aatccgagtc aacagacaga tatctatact
    25501 tattacaaag atgaaaaagt ctctttgaat tcaattggtg attcaagaag tgttacaaat
    25561 caaatcttag agtatcgtta tcaaaataaa ataaccgaga caaatacttt actacaaaat
    25621 agaaatacac aaattcgaaa tgtagagatg agttatcaaa tcaactcaaa cttttctgcc
    25681 aattttaaat caacccaatt tgatatattt gatgatcggt taaaacagta tccgactacg
    25741 atggctggta ttagttttac aagtaaatat gtgtccacag gttttatagc tggggagtct
    25801 agtttcaaca atccgtatcg atacaatcaa gttggatttt tgaatggaaa caattattat
    25861 cgtgacacag aaattcagct tagagataca gatcgtgcaa atagaaattt atttgagtgg
    25921 caggcaaata tttctccaac aaaaaattta atgttccaaa ctgctgtata caattcgaat
    25981 cgctctttga ttaatgaaac aaatgtttcc gagggagttc gaatcgcaat ggctattgga
    26041 ctcaaatata tagtattgaa ttttaaatac aattatctat ctgataattt aatgagatct
    26101 cttatgagac ctgattctcc ttcgtttttt aataaagact atgctgcatt tggtctgact
    26161 ttttttattg ataaaaataa aaagtattct atatatatag gaaataacgt ttacaatatt
    26221 gcaactatga cgaagccgac cgattctagt tccactgttc cggtgaataa ttcattttca
    26281 gcatctttta gaggaagaaa tagtgatttt aatactactt tcttttttaa ttttaaaaac
    26341 aatgcaaata gagagtttta ttattctaat tttggagtat ttagattgcc tatttcttct
    26401 caagtaaact ttgactatgc aacttctatg ggattagagt tgtcatttta attttaggaa
    26461 aatatacatg ttaattagtt ctgatttaat tctttcaaaa acttatcaat attttcatac
    26521 ttcaaagagg ttgaaagttg ttaaaaattt aactgctaaa cttgttctat actcaatgac
    26581 ttgttggctc attttatatt gtgctagttc tgatactaat cgtaggttaa taactgttaa
    26641 tccttccgat ctttactggc gtgatttgtc tttattctta tctggactcc caattgacga
    26701 gaaaaatcga ttttataaat taactgaact aagtagttat aaatcgtatg caaagacgat
    26761 agactcctat tggacaaaga ttcagtctga ttatttggat aaagttagac cctttaagaa
    26821 acaatacatg cctgattttg caaagtcaaa tacagcattt tatccactca gtggagcaga
    26881 ttttattaat ctaaatgcat tctacccaga tgctatatca aatatcatgg ttggacttga
    26941 acctgctggt aaaatatcag atccaaattc tttaacacag gatcagctga agaaaggact
    27001 tgattccatt cagagtatgg ttggagagat ggctgcccaa aactatttta ctagaaaaag
    27061 aatgcgaaga gagtttgcaa atcctcattt ctctggaaca tctcctgttt tattaatctt
    27121 tttaacacgc cttggactta ccattgattt ctgggagagg gtagagcttt cggctaacgg
    27181 taaactaatt cccgaaacac aagctcatat agaggataaa aatccagata aagttgaagc
    27241 ggtcagaatt tattatcaca tgatcaatga taaatacagt cgtgaattgg tattttttag
    27301 aatgtatatc aatgaaaatt cagccgagga atcgacttcg gaagggaaat tctttttgtc
    27361 gcaaggaaga tttaatttgt tactgaaatc tgctgaatat gttttgcaga tgtcacaata
    27421 tagaaagttt attgaaactt tgctttcaag aactgatatt gtagtgcagg atgattctgc
    27481 aattccatat aacgcttttt ccaaagatat ttggagccct cagctttttg gtatttatac
    27541 agctcgagct cggctacaaa atactccaaa cgttccggat caggaagatt taataaaaga
    27601 ttttaaagca gatgcgaaac cacttccttt taaatatggc tatggagttc taaaaggaaa
    27661 tgataaatcg aatctaatga ttttatccag aaagcaaaaa taattaacat aagctcaata
    27721 taccaattcc caaaagtaaa ttccgattgg aaactagaaa accgtccttt gggaattgga
    27781 aatacccctt ttgtgaaaaa tagcagaact tatttatgag aatggtatat aagaattttt
    27841 caccaccgat acacatcgat aactgaacga tacgaaacga tggataattt tgaattatcg
    27901 tatcttttat cggtggtaat cttttaattt tctgttacgc cgaactgatg ttaattatat
    27961 cgcttgtgaa aatactcttg tttcaggcga tttagttctc aaacgtagat caagccccat
    28021 gcatatattt cttagaaatg gtctacctga ttcctcaatc gaaagtgtat tttgattcca
    28081 agaaataaga ccatcttctt ccattgttcg aagatattct tttgtatttt ctacaagcga
    28141 acttggaaga ctaacgttcc attgtgtggc taattgcaga ataagtgatc tttgttctag
    28201 gtcttccttt gtaagcttat gacctcgaag agtagggagt tcccctgcat ctaaacgttt
    28261 ttgatatttc tttagaattt tttcattttg atgaaaacaa gtccagctat cagagattgc
    28321 agatactcca agtccaagtt gaatatctgt tttgtattct gtataaccca taaagtttct
    28381 atgcaaagtt tttgtttcat aagctttcca aagtttgtct gttgttagag caaaatgatc
    28441 cattccgatt tctctataat tggaactttc aaatttaagt ctagactctt cgtataacgc
    28501 tcttttatct gctcctttag ggagatctgc ttctgtaaac aatctttgag aggcttttat
    28561 ccaaggaaca tgtgcataac tatagaatgc aattcgatct ggctttaagg ttaacgtttg
    28621 ctctatcgtg cttgccatgg actcgcttgt ttgtttgggc agtccataaa ttaaatcaaa
    28681 gtttacagaa gagtaaccca actctcgagc tgtatttgta atatattctg tcttctcgaa
    28741 cggttgatgt ctgttaatca ttctttgcac ttctggatta aagtcttgca caccgagact
    28801 aattcgatta aatcctaaac ttcttaaagc ttctaattgt tcctttctag ttcttctagg
    28861 gtctacttca atcgcaaagc taggatcttt tactacattc attttggaga gaataggagt
    28921 aagaagtgct tttagattgt cgacagacag ataggtcgga cttccgccac ctaaatgaag
    28981 ttcttttatt ggtcgttttg tgatctcggg aaccctgtcg atatacattt gaaattcttt
    29041 taaaagattt gcgacataag gttcttccac agaatgattt ttcgtaatag aagtattgca
    29101 tccacaaaat gtgcataacg tttcacaaaa cgggatatgc agatataagg aaatagctgc
    29161 tgtttcgggc tcaagtgatt ttcgaatcgc atcaagccac tcttctgtag tagggctatc
    29221 tgaccaatac ggaacagtag gataacttgt ataacggggc gctggaatgt cgtatttagc
    29281 aattaatttt gatctgtcca ttaagcaaat tcctctctta ttgtttctac aaataatttg
    29341 acgttagttt ctggcgtttt gggtaaaact ccatgaccga gcccagaaac ccaaccgatt
    29401 ctttctttag gagaaagttg tttcatttca ttgatataag attttaagac ttttttaaat
    29461 tcagatgtct ctaaaaataa aagagattga tcgaagtttc cttgggtaaa tccaaattta
    29521 ttttctctga ttaaattggt aagtggaagt ctatgatcaa agcctacacc tgccaaatta
    29581 ctttgagtta gaacttttct tactatagtg tctgtcgtat tcttggagta atatcctatt
    29641 tttttaggga aagttttggc aaagtattct acgtatggaa atacaatttc attaaacatt
    29701 gagttggaaa gctctccggc tgctgtgtca aagatcatga ctacttctgc gcctgcatct
    29761 agttgtaatt gaatgttttg tgtaagtata tcttgcatga gagagaaaaa cttttttcta
    29821 gtctctgtta atacttttgc ttgtgtaaga cttccatcgt gtttcccgat gactgcataa
    29881 gtaaataacg tccatattcc acccacaaaa ccaattacag atttattttt aggaatgact
    29941 tccctagtca gtcgaacaca ttctttttga aattgtaaaa gttcaatagc atcagtggca
    30001 gatttaagtt tgtcaaagtt cgttgcatct aaatgccaat cgagtctcgg accttggtct
    30061 gtgtatttta atcccattcc aagtccttct aatggaaata gtaaatcaga aaataaaata
    30121 gctaaatcaa agtcgaaatc ctgaaccgga cctaatgcta cttccgctgc aacttcagga
    30181 atcttgcaaa gttccataaa gctatatttg gatcttagat tttgatagtg ggaatgatag
    30241 cgaccggctt gtctcatcat ccaaacgggt ggtatatttt gttcaatgta atttattgca
    30301 tttgtaaatc gttcactcgc cactgaattc tccttcttct cccatccatc gatagcctac
    30361 acttcggatc gatttaatgg ctttaagtcc atgttttccg agtgactgtc tgagtcgaac
    30421 aattgaattg tctatagtgc ggttcgatgg aaatttttct tcc
//
LOCUS       DAOQOS010000065        30179 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_680_length_30179_cov_6.908578, whole genome shotgun sequence.
ACCESSION   DAOQOS010000065 DAOQOS010000000
VERSION     DAOQOS010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 30179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30179
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_680_length_30179_cov_6.908578"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            247..2700
                     /locus_tag="PLF66_14720"
     CDS             247..2700
                     /locus_tag="PLF66_14720"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HMZ65347.1"
                     /translation="MDKISQFTTFLRDRYYNNIKAHRILPIEEGVYSDFPDGLHASLQ
                     NILQAGGIKSLYGHQISAFQSISSGNDTLMISRTASGKTLSFLLPILNDYISGKQPFT
                     TMLLYPTKALSRDQEGTLGKLMHAVASGRKLGTFDGDTPREEREKLKDNADFIITNPD
                     MLHSGILPNHNRKWKNFLSRLKYVVVDEVHTYRGAFGSHVANVFSRLIRICRLHGANP
                     IFICSSATVGNPSEHVEALFQRSFTVIDNDTSPRPKRDLFFLNPSLYKGIGGEMYRKG
                     PSSLSIPLIRYASENNIRTICFCKARQEVERLYTAVLESAPHLRSKIRPYRGGLLPNE
                     RRQIESDLFAGKINTIISTNALELGIDIGDLDLCILSGHPGTLASFWQQAGRVGRKGN
                     GAAIVFIAKDSPIDQYLVNHPEFVTSAPLEEAWLSSHNPYIILQHLPCAAYEYPLQME
                     ESQYAGDTYDFAVDVLVKNLTLKPYHESFRYNLQKYPAMGVNLRGMTDYNVEIFHNGK
                     VIGEIDPISARATLHKDAIYQHLGKRYMSINLDLEKKLCEVEEVNVDYFTEAVWETMI
                     QMTDVDEDKTLNGALLNFGEVNVNKQPKLFKKIKERTNENIGYGPITLPPFIYDTTGL
                     CLYPPKTWLEAVEAVDKRYSEAALYGLSYILKRSAPSLCMADVNDIQTDVSLSATARN
                     EWKSALYLYDAHEGGVGYAEKIYDKISDALTLCLAIIEECECEAGCPSCVTANPTGLE
                     NTDLQELLEESNASVACTRSLIQALLTGNFDLPEVILHKQAPPKKVDKVELDPETIKL
                     QKRLNRASSILQKKRDRVY"
     gene            complement(2697..3107)
                     /locus_tag="PLF66_14725"
     CDS             complement(2697..3107)
                     /locus_tag="PLF66_14725"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HMZ65348.1"
                     /translation="MTSKYVLDTNVISEFTKPKPNPKILSFLEKEKDNYCICAPVYQE
                     LLFGIELLVPSKKKNMFLEFIRDVVDSLDVLSYDLESSKIHAKLRAQLQNKGKTLPYV
                     DLEIASICIRNHSTLVTQNPKDYKAIPDLKLKVF"
     gene            complement(3104..3418)
                     /locus_tag="PLF66_14730"
     CDS             complement(3104..3418)
                     /locus_tag="PLF66_14730"
                     /inference="COORDINATES: protein motif:HMM:NF014643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HMZ65349.1"
                     /translation="MDWSENTYIPKEQFVETQPDVTVAEAKNHLPKLIHNSETNGVIP
                     ISRHGKVVAYIVSERDFRKLTQSTNGFLDKLKSFREKHNFIDEDIWSNVRSKETGREI
                     VL"
     gene            complement(3735..4364)
                     /locus_tag="PLF66_14735"
     CDS             complement(3735..4364)
                     /locus_tag="PLF66_14735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65350.1"
                     /translation="MRIIAFLLLLSLINCWKVYTRNETVLDKQTLETKEIKEQKDVFI
                     LSHSLTPKGLFLFLDAYKVEEQSQIDFIREKYKENKDFTFNDKYNFQEKCKGCGTGGL
                     FILPIVVSVILIELTTIPFRLVSFPQERIKEEIQKGNVRKIQSISTNDIYFAILDKNS
                     NRKYKFQSDRIFIPIAELKLDSINSRKFPYQLIDSRTNDILLTGNFDFS"
     gene            complement(4377..5354)
                     /locus_tag="PLF66_14740"
     CDS             complement(4377..5354)
                     /locus_tag="PLF66_14740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65351.1"
                     /translation="MKFIVTILIVMNVTYLSGESKSQKERVLAGLILRTGDIVDSIRP
                     IYANVNKTGKLDDLSFGGEQVGGKGGSERIVLKNGYVIVGFILDKGNYFGASHIARLR
                     VVWQKWNRNKPEGDLILSDSFSSGNNLMDAVKLELIAPSASYIYELSATATEHTNGDN
                     FLSDIQISTINENKLRKNLNTSPIFLTPITYEIDIEIISGFNRGKKATGFFTYNTSNI
                     KGEGEEFIEVSDIEFIYDKDAYTRDMFDSVPKVRLLNGEFKELQLVGGPQTKRFGINS
                     GFNRNQFGRDSEKFILNGEDYFGYLNADTYVEGAGKVKYTKQIASEQSR"
     gene            5573..6490
                     /locus_tag="PLF66_14745"
     CDS             5573..6490
                     /locus_tag="PLF66_14745"
                     /inference="COORDINATES: protein motif:HMM:NF013559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M12 family metallopeptidase"
                     /protein_id="HMZ65352.1"
                     /translation="MKIKSLFAMLLLSTLLLPNCNSEKKDITEIQRNLLIYLISPPHS
                     NISQSSPNPNIYQTIFTNNCKTKSISIQGGNVDNFDYPPTVDDMIFSTEETTDELELR
                     SASISGCSNLWNGAVIPFEIDPQFSNVNLLYSAFATWESQTVIRFIKRTNLHRDYIFI
                     IPSNECSSFVGKRGGAQNVNLSSSCTYKNILHELGHVIGFYHEHQRIDRDNFITIHTE
                     NIQPNKLSSFKKLFPSYSFSISYDLYSIMHYTPYAFSYNSKPTITLKDQSANFGNLGT
                     LSTIDVNIVNQLYQNSFTTINNIPAVYSR"
     gene            complement(6492..7793)
                     /gene="purB"
                     /locus_tag="PLF66_14750"
     CDS             complement(6492..7793)
                     /gene="purB"
                     /locus_tag="PLF66_14750"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004787068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HMZ65353.1"
                     /translation="MIDRYSNPEIAKIWSLENKFEIWKDIEILACEARKDRGEIPEAD
                     FNDIKTKAKFKVEEILKLEETLQHDVIAFLTNLGSYIGPASRHVHHGLTSSDIGDTAL
                     CVQMKQAIEIIIKRVETLLETVKEKAIQEKNSPCMGRSHGIHAEPMTLGLKFALFYAE
                     MQRNLERLKQAQKEISVGKFSGAVGTYSNIDPAIEEFVCTRLGLAVDPITTQVITRDR
                     HGFYLSVLGLTASSLDRMATEIRLLQKTEGREVEEPFTKGQKGSSAMPHKRNPVICER
                     ISGIARVIRSNVNVGFQDMQLWHERDISHSSAERIVLPDSTIALEYILDKMNYVIKNL
                     HIYPDASQRVIGVTRGLIFSQKLMLAMIERGGISREKAYEKVQSHAMAVWADQSQTMK
                     SRLLADPEVNQVLTAEVLDEICDIKPYLARVDIIYKRLGLV"
     gene            complement(7790..8266)
                     /locus_tag="PLF66_14755"
     CDS             complement(7790..8266)
                     /locus_tag="PLF66_14755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002746546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbuC family cupin fold metalloprotein"
                     /protein_id="HMZ65354.1"
                     /translation="MKEKVQLITEDLFSSVSEKAKLSPRKRANHNFHELTEVYQRFLN
                     VLTRGTYVQPHKHENPPKPETFIALKGSLGFLIFDDVGNITGKYVLSDNGPIRGIDVQ
                     PGVWHGLVTLSDVCVCFEGKSGPYDPKEDKFFASWAPTEADDARFDTIKYWESLFK"
     gene            complement(8450..9664)
                     /locus_tag="PLF66_14760"
     CDS             complement(8450..9664)
                     /locus_tag="PLF66_14760"
                     /inference="COORDINATES: protein motif:HMM:NF024571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HMZ65355.1"
                     /translation="MNSRYIETLLSEISFGQNKMSFISGPRQCGKTTFGQMLLKERGI
                     GNYCNWDDIEFRRIWAKSPKSIVPVGKKHTPILILDEIHKSRLWKRELKGLYDTLASG
                     LDILVTGSARLNVYNRGSDSLVGRYYHFRMHPFSLGEMLGYLPLAPDEFILSLKNRVR
                     KHRKAHITAFNALFKFGGFPEPLFAEDERKARLWRTSRIERVLREDLRDLSRIQEIGK
                     LEMLAALIPERVGSLFSRASLREILELSFDTVTRWTEALKELYYIYEIKPWQRNISRS
                     IKKEGKVYLWDYSEIPSPPARFENLIASHLLKACHYWTDGGYGNFELCFIRNKEKEEI
                     DFLVTKDKNPFLAVEAKWNEKEISPHWKKFHPHLKDAYCIQVTKEEIEWKKTSNSSIN
                     VCSASEFLSYLF"
     gene            complement(9760..10842)
                     /locus_tag="PLF66_14765"
     CDS             complement(9760..10842)
                     /locus_tag="PLF66_14765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017862372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HMZ65356.1"
                     /translation="MNPREFFIEDRLEKFRLTVCCNLGESGLRNFTVGEIFDKINLPI
                     DIMRGISLADSPNRGREDLRTEIASLYNNVSPDQVLITSGTSEALYIIFHLIIEKGDV
                     VSYFSPAFQALYEIPSMLGAELNPVLVKDSLDVKELFQDNVKLAILNHPHNPTGIGLQ
                     KEDIDLLKEIILNYKGYTIFDEHYRFLDYNSELGFSGALLNEKCIATGSITKCFGVTG
                     LRIGWIVADKKLIEKARSFKDYLTHTVNPISEFIAYEIIKKRKEILTPIKYSIQQNIE
                     IFYKAAHDLPFIQSFRKPDGGLVSIAKLKEGVLSEYYSDELLKQCDIFVLPANNFEQE
                     GWIRIGFGETPERFKAGIERWKYLKI"
     gene            complement(10839..12074)
                     /locus_tag="PLF66_14770"
     CDS             complement(10839..12074)
                     /locus_tag="PLF66_14770"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HMZ65357.1"
                     /translation="MLGNLKNVSILIVDDTPKNIQLLGTILKNAGYKVNVATNGLQAL
                     SVLEKLKPDLILLDVMMPDLDGYETCKRIKEINHLKDIPIIFLTAKTQTEDIVKGFEL
                     GAVDYIVKPFNALELLARVKTHLSLKFNQEMLQTLLNFQKDIVIMTDGERVLAANQSL
                     LDFSRVDSLDTFNMNYPHILDFLLLENEIDKEANLRLTKLPKDEELKVKIRNPKGEFL
                     IFRVKQNIIPEKGVSILNLTDITLFENQKTTLEEKASLDELTKVFNRSKFQQLFKQTI
                     DENKFNPKSLCLVIFDIDHFKRINDTYGHNKGDEVLVSVSQFIKAQIRMTDILCRWGG
                     EEFTLLLVGSNIDAGVRICEKIRLLVETYSFLEDRPVTISIGITQYQEDDTLESFIAR
                     ADNALYRAKKSGRNRTEKN"
     gene            complement(12064..15141)
                     /locus_tag="PLF66_14775"
     CDS             complement(12064..15141)
                     /locus_tag="PLF66_14775"
                     /inference="COORDINATES: protein motif:HMM:NF024140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HMZ65358.1"
                     /translation="MSVTKYYITKVSWYNQLKIKKKALVGITAICFLILVGISLSIHL
                     VNKIHEETKEIHNKWMQSIIQISEMRYQIDQIYKFENKHVAADKISEKEMIEKRISNL
                     QIDLVGSKTYYRVLNYHDEEEKSFAKFRDDLEEYLKIHNQILEESRKFNKASIRSLQE
                     KSKTIVDQLNSDLYELLSLNQRGELESNQIVDKSYYSLIFQMILTSFFTVVFIYLVIN
                     YTFKQFVFPLQLAEAAANRIAKGDWKVKIEYDSNDELGNFIKTFNEMVEEHRKIDAEK
                     SRIEKEVRRSEELFRTLIEISPDSFLLIGLDFSVLWANNNMLRMFGYGSIDEIRELNA
                     VAFIEQDERGKIASIMKSLLSSDYSNYNYESKGIRKDNTVFPIEVQLSVYQNENVNVG
                     ILTNIRDISERKLAEQALIENEERYRIITENMADVILVIDARTMKLLYVSPSVQNLTG
                     YTVLEMMSIPINDLLSLRSLIYAKRAFPLRVKRLTAKKKNINDIYIDEIEQYKKDGTT
                     IWTEIVSRYVYNENTKSLEVHAVMRNISERKKIEREINEKSQILAGILANMPVIVFRV
                     NAEGLIIQSIGAGLRKMGLFDNQAVNINIFEVYSEFCPTLRDALQGEPQVFISNGLYN
                     DGNEWYFQNYFFQDEFTGGLIGFGLDITEQMIAKKAAEAASKVKGDFLANISHEIRTP
                     MNAIIGFTEILKGSIKDDIQKKYFENIVSSGKTLLTLINDILDLSKIESGKVELQTSS
                     VNIKEVFSDLKNIFIQKINEKNLDFQIIINENLPPEIILDEVRFKQIFLNLLSNAIKF
                     TEKGYIRLTARAISKVVDTTKIDLIFTVEDTGIGVPIDEQRAIFEAFTQQKGQNHAKY
                     GGTGLGLAISSRLVSLMNGSISIKSEVGKGSIFQILLKDVSISDESILEGKNDIILEK
                     PRIDLENQEEARHQILELDDAGIQNLKTLLVEMENDLYDEWKELSDASSINEIEEFAY
                     KIKYLGEKYNYPPLMRYSENLQAKALLFDMNSLIVLLRTYPELIQNLRSHYVG"
     gene            complement(15141..17600)
                     /locus_tag="PLF66_14780"
     CDS             complement(15141..17600)
                     /locus_tag="PLF66_14780"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HMZ65359.1"
                     /translation="MNGCNIGLALKDSPKVEAGVLNLTFWDFEKQGAVNLEGEWEFYW
                     KKIYRPSQTEISKELLSGYFEFPGYWNGRIVDKEILSGDGYATFKVKVMLPESMHSIP
                     KKKLALRMKEQATAYELYLGNELLTGRGRIGTNPKESTPGYGTETIFFETNQQSFELT
                     LIVSNYHHRNGGIWSIPILGTQDQILQLKYERNSIEFLLGGSILIMTIYHFMLFIFRK
                     NDLTSLYFASFCLVTLMRVISTGEIMIVQLIPNIHYEVVTKFEYMSFFLLISIFFKFF
                     YQLFPDDVSDLLVKISMSIGGVLSMIVCFSSLRLYNYFVNPNYVVIITIIVIIYIYQV
                     RLVVKRREDALVIFLFSNFLILFIVNDILYNLKVIVTGELMQIGVFIFLLAQSVILSR
                     RFSRAFRDIEDLSNHLTLVNAAYSNFVPREFLSILNKESITEVSLGNLAQRDVTILVS
                     GIKGFSAISERLSPKEIFELLNEYYIGITAIIRKKRGFIGKFIGDEIMVLFPDFPQNA
                     ILAATEMNQYTHSFNLRNKELGKSTFNIGVGIHSGNVTFGTLGGIDRMDTTVIGNPVE
                     MAQKLETLANSFFVNVLISSDTYLQLEKKERELCREIEYLKIQGTEGFLTVYEYFGAD
                     TEDMLKKKKNFNAEYFRGLTLFRGGFFDKAEEIFTKCKTILPEDPILALYINRCRDKT
                     LSDSVETVEANSNKPEIALIICRDEEFTIRLEKALKKEKLDVITTKTALEAISICSNI
                     KPKILFMNIHMVDMNAKDLIEKLRVEQNLSVDDTYIVAITSDSSQLVKSVIYESGADD
                     FIMDKFHPDSLNQIISRVLNR"
     gene            17765..19063
                     /locus_tag="PLF66_14785"
     CDS             17765..19063
                     /locus_tag="PLF66_14785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65360.1"
                     /translation="MIQRLFFLAIPISFILYCTTAKPVDIKKQNPKLYRELIRDYSVV
                     SEENPGKIETLKFIENETQSIVGKINNFPIESCTWFFDRKVKDKNNLDDTMFLQKAIN
                     LNCGGQYYIIELDKQNIQANKLKYQGVYKGIFDQLRKAIIVEASCSIYSTTNKEEDDK
                     NTAIECYPYPYDLERYTKNQKREVVAGYFLYTENQETLESLMMASKEEKDNTKVLQEL
                     KKDFVSETDANNQKTNPSIGKNFTFKNCEYIDFREAQTLNKKGKDLKAKIDKFVESDL
                     TDPTNKESLRDAIEEYRTCGNRCYSKDKEIQILFEIKNEEAKRKTLIIKKLSSRSELS
                     KYKRFNFYPVEGSLKSIDFSVKGDIEKIYLDPIIETTPTQSDIKPEPSKVIEEVKKAE
                     EEEKTKEKKKKKSKITETVVQPIPEPDAEGNTESVLPKQE"
     gene            19197..21185
                     /locus_tag="PLF66_14790"
     CDS             19197..21185
                     /locus_tag="PLF66_14790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015682196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FapA family protein"
                     /protein_id="HMZ65361.1"
                     /translation="MSSKLKAAMFEEESALEKLEKEQVEVIGDTIDESLRLAAKHFGK
                     ELYQLDYEVIERGKKSLFFSHPYRIRVFISNHEDNIQELAGIDKKLTGGSGKLLSTEL
                     KKLIVPKDKDGWVSIKYYRHGLYMKEIPPVGSGKYPTVQDIIKKLNLRGIAPPNEAAI
                     QSFLDSKSTEYVRFGDAKLKPFAEAGAKVEISDDNMKAFVTLLPPKLAGRDLEIADVV
                     YELKKEDIVYGIKEDVIREFLENERYNEPFVAAAGDPPIHGKNAQIIYHVRTEKKVVL
                     KEDANGKVDYKDLDLIENVVVGQLLAEKTPPEKGKYGRNLFNEMLEAKDGQDIPLNQG
                     KGTILSEDKMKLTAEVNGQVIFAEDRLSVETVYRISGDVNMRTGNITFLGSIVITGNI
                     EDNFQVKASGNIEIYGTVQKAQVEADGDIIIRQGVTGRGEARIESTGGNVVSKFLQDA
                     IVVTDKDVVVQEVIMNCDVSAGGKVTCNGKKANILGGRIRAGTLISAKNIGSPANPYT
                     ELIVGVNPKLLKQIEDYTAKRKESHDKLELLSKTYKTMKARQDDDPSSFSEENAAYLA
                     KLEVGIKKLEKRIAEYDKEIQTVNSYMEQAGDEGKVFFEKKMYGGVSVRIKNAEPYKI
                     KNDLQSKILFLSENKIQMKPFADPEAEGGQSKRGGRRK"
     gene            21202..22065
                     /locus_tag="PLF66_14795"
     CDS             21202..22065
                     /locus_tag="PLF66_14795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65362.1"
                     /translation="MELLALVIINIVFGVILYYAVSIKVTNSVRDYQIVKLKKEIQAH
                     TLTFFKESESYLALMDSRIAVLKNLLHKAEQMGIDFKNLPEQEKLAASLSTTNTESNQ
                     NKKPYVSKFDEEAIQDYPQKTVKLKPQSSVIKKTEEEDNGFFSGLIGGLGKGLKSIFG
                     INSDIMIDPNLDTNTKTKKNSVIDVSIGGNPLEATESVQDNLKTEESFQTVFNTVNNP
                     YIQPKQEDTLRISIKAALEELPADATKVEKVVHLLKKGYSHTDISEEMGLAIPEISLI
                     ETIKIERSRRI"
     gene            22067..22360
                     /locus_tag="PLF66_14800"
     CDS             22067..22360
                     /locus_tag="PLF66_14800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016560763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF370 domain-containing protein"
                     /protein_id="HMZ65363.1"
                     /translation="MSAFKILNIGFSNILMTSRVVAIIHADSASARRLRSEAKNSGHL
                     VDATNGRKTRSVILTDTNHLVLSSLKVDSLAKRIEADDNSLPALEEESLESEP"
     gene            22344..22937
                     /gene="gmk"
                     /locus_tag="PLF66_14805"
     CDS             22344..22937
                     /gene="gmk"
                     /locus_tag="PLF66_14805"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004785244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HMZ65364.1"
                     /translation="MKVSPRGILYVISSVAGGGKSTLISLLLNKYPDIHFSISVTSRP
                     VRPGDEPGKNYHFVSRDEFERLIAQDFFLEWALVHDNYYGTSKRYIEKELRKGYKVIL
                     DIDVQGFRIIKSKIQNIKSIFILPPSEEVWIDRLKKRGSDSPESIERRIQNGRKELQQ
                     AGDYDYQIVNDKLEDSFAELEKILFGDKPKEDSPQHE"
     gene            22930..23511
                     /locus_tag="PLF66_14810"
     CDS             22930..23511
                     /locus_tag="PLF66_14810"
                     /inference="COORDINATES: protein motif:HMM:NF015559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HMZ65365.1"
                     /translation="MNNSLSVQSGILKGKKIPIPSTVKGNSNFTSAILKKSVFSILDS
                     LHLQNLINLEESFFIDLFAGSGQMGIEAVSRGFKNSVFFELDKNRFRGLKDFLPTIKK
                     DFILLHRDSFRYYDSFDNEPVKSLVFYIDPPYSFWDLNLEKILRLTNDILNSYTQKNI
                     VLLVQSPFDPKWNSFEIRQVGNNYLLSLVNKIL"
     gene            23704..24600
                     /locus_tag="PLF66_14815"
     CDS             23704..24600
                     /locus_tag="PLF66_14815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65366.1"
                     /translation="METLKSLKIEKIYLRLFDVDRVGNINLPVGEVTIIKTPINILII
                     PVIYITNRSLVGLEKDSIQKLAVNVLKKTEKIMDNNHLPLKAIQFDCDWTDSTRNNYF
                     YFLKEIQNISKNKIALSTTIRLHQIKYREKTGIPPVQEGFLMFYNMGQINEASTRNSI
                     FNKEDASKYLSRLENYPIPLSYILPIFSWVVHFRDGRILDLITKKDMDSILNSTNLEK
                     TKSDDLFLVKESFLSEGRFFKVGDLLKKETISSIDLIEAAELLKNSNKQNNYDIIFFD
                     LDEINLKRITNETLNKTISIFN"
     gene            24563..26836
                     /locus_tag="PLF66_14820"
     CDS             24563..26836
                     /locus_tag="PLF66_14820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65367.1"
                     /translation="MKLSTKLFLFLISFLLTSISILIACGPYGWMDFSYNTLLTPEII
                     QNPKAEKFFWSYKYSLYKRFKDNYNTDFNSTNVSEWSNFFPSKVHEDKIKFWLYSSNL
                     KEIDEMIFALQGKGTVATENVKKFSLIGSATPNSCIEFLYYVGFARRNEEFTTNVIDL
                     YSWDEPKKTKDLSQLQKQIDGGLRFFEKEKNDFLKERFIFQLIRLYYFNSQYEEAISF
                     YKKNEAFFKTNNSMKARSLGYVAASHIKLDQIGQANYLYAYLFANEPIMQETAYLSFR
                     PQSGKDWEESLKLAKTKQEKTALWFLFGITFDPETGIQEIYKIDPQSDYLEVLLVKEI
                     NHLEEHFLLVQPSLPLVGINIEESHQFYSRKKSFLKTVTEIAKDDKIKNKFVWNISSA
                     YLNYTYSNFSEGDNYLAKAKTFIDKDVKNKLLYNSQFSIVFAYGKLLSNSKLTPSVEA
                     EILPHLKTIFSKQIESIPNFRYDYPRFWISNAIAVRYASQGEYEKAEVVQNGTLRLHF
                     SKLENTKKMISYFDRKDHSELEKFYQARSSLKKKHYQQLLGIHYALRDELELSLETFK
                     SIPNYKYTFQTDPFAIRKKDCYECDILPKSAKKYDSIYFIQKLIDLKKEAVQNKNKQA
                     ENYFLVANAFYNMSFFGTSKFYRNDVHSFKQNYEMWQYSKFSKMDFPEMDNSTALKYY
                     LLAASASNDPEFKAKMNFFAAKAELNEWHVQNKNYDKHFQSGLYYSILKKNFSNTRYY
                     SEIIKECIYFARYINGK"
     gene            complement(26993..28006)
                     /locus_tag="PLF66_14825"
     CDS             complement(26993..28006)
                     /locus_tag="PLF66_14825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001011676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HMZ65368.1"
                     /translation="MRFKKEIQLTDRVISKDHKPFLVAEIGLNHNNDLEIGKKTIAAA
                     KKAGADAVKFQSYVTEEFIDPRSSDAKFLFDIFKQYELSEHFHREFQKTALNEGLIFF
                     STPLCVTSVDLLLNLKVPVLKIASGDIVNSELLEKCASSGLPLFVSTGAADLYEVTRA
                     VEFLETKNVSELCLMHCVSLYPTPPEKLNLKTIQLYSSMYDFPIGFSDHSAGALGAAV
                     AIGYEACVIEKHFTLDRSLPGPDHSISINPEEMKQLADNCNFAFQMKGEKIKKVTEEE
                     FKGRFFGRRSLYLHPEKQKPIAMRPALHVKDDYSVDAWSHLTFKVRDFNKTKPGEPIR
                     LDI"
     gene            28088..28642
                     /locus_tag="PLF66_14830"
     CDS             28088..28642
                     /locus_tag="PLF66_14830"
                     /inference="COORDINATES: protein motif:HMM:NF024436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HMZ65369.1"
                     /translation="MKVIFIIIFLFISCASTRYINPEESEPTREWGPTEISATVDTMV
                     VSVQNYFETTKEKPYIELNKIQNKTSEHIDTGMLANEISTNLIKRKIVFVDRSHRGDA
                     LKEMELGQRGIVSANSALPTGEFLSPNFKLNGEITDNVRYVNEEKVQYIVVTLRLLKL
                     STGGVVWQDEKKFLKVSTNQKFGW"
     gene            28686..29006
                     /locus_tag="PLF66_14835"
     CDS             28686..29006
                     /locus_tag="PLF66_14835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HMZ65370.1"
                     /translation="MPATVLPDTDIDTEIKEGNRGGPWKVVLWDDDEHTYDYVIEMLM
                     EICQMSIEKAFHHALEVDTEKKTIVFEGELEHAEHVQDRIHAYGPDPRMAESKGPMSA
                     TLEQ"
     gene            29104..29856
                     /locus_tag="PLF66_14840"
     CDS             29104..29856
                     /locus_tag="PLF66_14840"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HMZ65371.1"
                     /translation="MINKKKVIVVLPAYKAALTLEKTYKEIPFDIVDDVILVDDASPD
                     NTPEVAKKIGIKHVIVHEKNKGYGGNQKTCYNTALKLGADIVIMLHPDYQYTPMLITA
                     MASIISLGLYPVVFGSRILGTGALKGGMPLYKYFFNRCLTLFQNILLGQKLSEYHTGY
                     RAFSREVLLKTNYEVNSDDFVFDNEMIGQIFYEGFEIGEVTCPTKYFPEASSINFKRS
                     VKYGLGVLSVSLKFRIQKMGLFKFKIFEKKPA"
     gene            complement(29873..>30179)
                     /locus_tag="PLF66_14845"
     CDS             complement(29873..>30179)
                     /locus_tag="PLF66_14845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65372.1"
                     /translation="IYEYYKVPTIFQNLFSDDEEIVKESYWLINSKRNEIYQNFLIEL
                     YGKNYKPEQLSFDLDEIKKMILSLERSDWKKIGKIYFTVFLIVSLLLFYLLLPDEVI"
BASE COUNT        10257 a         5336 c         4680 g         9906 t
ORIGIN      
        1 ctccccatca gccgagctat accgcaggct attgaattta ttcatttatt agctttattt
       61 aatattctaa tttcctttat catacttatc aatttctatc ttttgcgtaa ggtcagttag
      121 atataatttt cacgcgaaaa gaatcgatat aagaaaagta ttgcttttct tcctacacat
      181 ctcggttaac aaaaactaaa tttattttct tgacctccta tatgattagc aaaattctat
      241 ttagatatgg ataaaatttc acaattcaca acatttctaa gagatcgtta ttataataat
      301 attaaagcac atagaattct tcccatagaa gaaggagtct attctgattt ccctgatgga
      361 cttcatgctt ctttgcaaaa tattttacaa gcaggcggaa ttaaaagttt atatggtcat
      421 caaatatctg cctttcagtc tatatcttct ggcaatgaca ctcttatgat ttctagaaca
      481 gcaagtggca aaactctttc ctttctactt cccattttaa atgattatat atctggcaaa
      541 caaccattta ccacaatgct cctttatccc acaaaagcgc tatccagaga tcaagaaggg
      601 acgttaggaa aactaatgca tgcagttgca tctggtcgca aactaggaac gttcgacggt
      661 gacactccaa gagaagaaag agaaaaacta aaagacaatg cagattttat tattacaaat
      721 ccagacatgt tacattcagg aattttaccc aatcacaacc gaaaatggaa aaactttctc
      781 tccagactca aatacgttgt agtagatgaa gtgcatactt atagaggagc ctttggttcc
      841 catgttgcaa atgtgtttag caggctaatt cgcatttgcc gcttacatgg agcaaatcca
      901 atttttatct gttcctctgc aacagtagga aacccttccg agcatgtaga ggcacttttt
      961 caaagaagtt ttacagttat tgacaatgat acctctcccc gtcccaaaag agatttattt
     1021 tttctaaacc catctttata taaaggaatc gggggagaaa tgtatcgaaa aggtccatcc
     1081 tctctttcga taccactcat acgttatgca tctgaaaata atatcagaac catctgtttt
     1141 tgcaaggcaa gacaggaagt tgaaagactt tatacagcag ttctagaatc agctcctcat
     1201 ctccgatcta aaattcgacc ctatagagga ggtcttcttc caaacgagcg aagacaaatt
     1261 gaatctgacc tgtttgcagg aaaaatcaat acaattatat ctactaacgc tcttgaacta
     1321 ggaattgata ttggagatct agatctatgt attctttcgg gtcatcctgg aacccttgca
     1381 agcttttggc aacaagcggg acgagtcggc agaaaaggaa atggtgctgc gatcgttttt
     1441 attgcaaagg attcgcctat cgaccagtat ttagtaaatc atcccgaatt tgtaacgagt
     1501 gctccactag aagaagcttg gctttcttct cacaatcctt acattatctt acaacatctt
     1561 ccttgtgccg cctatgaata tccacttcaa atggaagaga gtcaatatgc aggagatacg
     1621 tatgattttg cggtagatgt tttagtaaaa aatttaacac tcaaacctta tcatgaatct
     1681 tttcgttata atttacaaaa gtatccagca atgggagtga accttcgcgg tatgactgac
     1741 tataatgtgg aaattttcca taatggaaaa gtgattggag aaattgatcc aatcagtgca
     1801 agagccactc ttcataaaga tgccatctac caacatcttg gaaaacgtta tatgtcaatc
     1861 aatctagatt tagaaaaaaa actatgcgaa gtcgaggaag taaatgtaga ttattttact
     1921 gaagcagttt gggagactat gatccaaatg acagatgtag atgaggataa aactctaaac
     1981 ggtgcactct taaattttgg cgaagtgaat gtaaacaaac aacccaaact tttcaaaaaa
     2041 atcaaagagc gcacaaatga aaacattgga tatggaccaa ttacacttcc cccttttatt
     2101 tatgatacaa caggactttg tctctatcct cctaaaactt ggctagaggc tgtagaagca
     2161 gtggataaac gttattcgga agcagccctg tatgggctta gttatatttt aaagcgatcc
     2221 gcaccaagtc tttgtatggc ggatgtaaat gatattcaga cagatgtatc tttatctgct
     2281 acagctcgta acgaatggaa atctgcactc tacctatatg atgcccatga aggaggagtt
     2341 ggctatgcag aaaaaatcta tgataaaatt tcagatgcac ttacactctg ccttgcaatc
     2401 attgaagaat gtgaatgtga ggcaggatgt ccttcttgtg tgactgcaaa tccaactggt
     2461 ttagaaaaca cagacttgca agagttgctt gaggaatcta acgcatctgt ggcttgcact
     2521 cgaagcctaa ttcaagccct cttaacgggt aacttcgatt tgccggaagt gattttacat
     2581 aagcaagctc cacccaaaaa agttgacaaa gtagaactcg atccagaaac gattaaacta
     2641 caaaaacgac tgaatcgagc atcttctatt ttgcaaaaga agagagatag agtgtattaa
     2701 aacactttca atttcaaatc aggaatggct ttataatcct ttggattttg agttacaaga
     2761 gtagaatgat ttcggatgca tatagatgca atttctaaat ctacatacgg aagagtcttt
     2821 cccttgtttt gcaattgggc tctgagcttt gcatgaattt tagaggattc aagatcgtaa
     2881 cttaacacgt ctagtgaatc gaccacatct cggataaatt ctaaaaacat atttttcttt
     2941 tttgaaggaa cgagtaattc aattccaaaa agcaattctt ggtaaacggg agcgcaaatg
     3001 cagtaattgt ctttttcctt ttctaaaaag gaaagaattt taggatttgg tttcggtttt
     3061 gtaaattcgg aaatgacgtt tgtatctaaa acatatttag aagtcacaga acaatctctc
     3121 ttcctgtttc tttgctacgc acattactcc aaatgtcttc atcaatgaag ttgtgttttt
     3181 ctcgaaaaga tttaagctta tctagaaagc catttgtgga ttgggttaat tttctaaaat
     3241 ctctttctga gacaatatag gcaactactt tgccatgacg gctaattggt attaccccgt
     3301 ttgtctctga attgtgaatc aatttgggca aatgattctt tgcctcagcc accgttacat
     3361 ctggctgtgt ttccacaaat tgttctttcg gtatatatgt attttcactc caatccatat
     3421 agctataatt atagccttat ttttatttgt caacgatttt tacttttttt ctcactttta
     3481 agtaaatagt agcatccttc gaaattaaca actcccctta cgcacttcgt gcgtaagggg
     3541 agttgtttga cagggagcct gccggcagct tggggactaa tcgtccccaa acccctattt
     3601 tcattaactt attagtattt actaatattc ttttttttat tttaaatatt aatactaaat
     3661 ttcactttaa gtaatataag ttatttaaat gattttaatc tgacaggttc aaattctttg
     3721 aatttagaaa tgctttacga aaaatcgaaa ttgcctgtaa gtaaaatatc gttagttctt
     3781 gaatcgatca attggtaagg aaattttctt gaattaatgg aatctaattt caattcagca
     3841 atgggaataa agattctatc actttggaat ttatacttcc ggtttgagtt cttatctaat
     3901 atcgcaaaat aaatatcatt tgtagaaata gattgaatct tacgaacatt tcctttttgg
     3961 atttcttctt taattctttc ttgtggaaat gacaccagtc gaaaaggaat cgttgtaagc
     4021 tcgattaaaa ttactgaaac tacaattggt aaaatgaaca accctcctgt cccgcaacct
     4081 ttgcattttt cttggaaatt atatttatca ttgaatgtaa aatccttatt ctctttatat
     4141 ttctctcgaa tgaaatcgat ttggctttgt tcttctacct tgtatgcatc tagaaataaa
     4201 aagagtcctt tcggtgtcag agaatgcgat agtataaata catctttctg ctctttgatt
     4261 tcttttgtct caagagtttg tttatctaaa acggtctcat ttctggtata aactttccaa
     4321 caattgatta gagagagtag aagtaggaat gcaattattc tcatagtatt actttttcat
     4381 cggctttgtt cgcttgcaat ctgctttgta tatttaactt tacctgctcc ttctacataa
     4441 gtatctgcat tcagataacc aaaataatct tcgccattta aaataaattt ttctgagtct
     4501 cttccaaatt gatttcgatt aaaaccagaa ttgattccaa atctctttgt ctgaggtcca
     4561 cctacaagtt gtagctcttt gaattctcca ttcagcaagc gaacctttgg aactgagtcg
     4621 aacatatcac gagtgtaagc atccttgtca tagatgaatt ctatatcaga aacttctata
     4681 aattcttccc cttctccttt tatattggat gtgttataag taaaaaatcc tgttgccttt
     4741 ttgcctctat taaaaccaga aatgatttct atatctattt cataagtaat aggtgttaga
     4801 aagataggcg atgtatttaa attttttcta agcttatttt cattgattgt agagatttgg
     4861 atatcagata aaaaattatc tccatttgta tgctctgttg cagtcgcgct caattcatat
     4921 atataagaag cgctcggagc aattaattct aatttgacag catccattaa attgtttccg
     4981 ctgctaaaag aatcagataa aatcaaatcg ccttctggct tatttcgatt ccacttctgc
     5041 caaacaactc taagtcttgc aatatgactt gctccaaaat aatttccttt atctaaaata
     5101 aacccgacaa tcacatatcc atttttcaaa acaattcttt cagaccctcc ttttcctcct
     5161 acttgctctc caccaaaaga taaatcatct aattttccag tcttatttac attagcgtat
     5221 atcggtcgta tcgaatctac aatatcacct gttctcagta tcaaaccagc taagactcgt
     5281 tctttctgtg atttactttc tccagagaga taagtcacat tcatcacaat taaaattgtt
     5341 actataaatt tcatatatcg tatttgattt ctccttttat aagaactatt gttacccaag
     5401 tctttaaaga aacaatcatc cattttcttt tatggttacg ttctaatgag aaaacgaaag
     5461 actaatcctt gtcaatttat ttcgataaat tctctttcat tcgataggtg attcccgttt
     5521 ttgcggctat cccctattgg aaaatgaatt tatttgaatt agtatacatt ctatgaaaat
     5581 taaatcatta tttgcaatgt tattactttc gacactgctt cttccaaatt gtaactctga
     5641 aaaaaaagac attacagaaa ttcagcgcaa tctcctgatt tatctgattt ccccacccca
     5701 tagcaatatt tcacaatcaa gtccaaatcc taacatttat caaactatat tcacaaataa
     5761 ttgtaaaaca aaatctatct caatacaagg aggaaatgtg gataattttg attatccacc
     5821 aacagtggat gatatgattt tttcaaccga ggaaacgacc gatgaactag aattaagatc
     5881 ggcttctata tcgggctgtt ccaatctttg gaatggtgca gtaattcctt tcgaaattga
     5941 tccccagttt tcgaatgtga atttacttta ttctgccttt gcgacatggg aaagtcagac
     6001 tgtaatccga tttataaaaa gaacaaatct acatagagat tatattttta ttataccttc
     6061 aaatgaatgc agctctttcg ttgggaaaag gggtggagcg caaaatgtga atctatcttc
     6121 atcctgcact tataaaaata ttttacatga gctaggtcat gtgataggat tttatcacga
     6181 gcaccagcga atcgatagag ataatttcat taccatacac acagaaaata ttcaaccgaa
     6241 taaattgagt tcctttaaaa aactttttcc aagttattct tttagtataa gctatgatct
     6301 atactccata atgcattata ctccctatgc attttcttat aattcaaagc caacgattac
     6361 actaaaagac caaagtgcaa actttggcaa tttaggaact ctttctacta tcgatgtgaa
     6421 tattgtaaat caattgtatc aaaatagttt cacaacgata aacaatattc ctgcggtcta
     6481 ttctagataa gctacactaa acctaatctc ttataaatta tatctacacg agcaagatat
     6541 ggttttatat cacaaatttc gtccagaact tctgcggtta atacttgatt tacttcaggg
     6601 tcagcaagta atcttgactt catcgtctgc gattgatctg cccatacagc cattgcgtga
     6661 gattgaactt tttcgtaagc tttttctcgg gaaattccac cacgctcgat cattgcaagc
     6721 attaacttct gagaaaaaat caatccacga gtaacaccaa ttaccctctg acttgcatca
     6781 ggatagatat gtaaattctt aatcacgtaa ttcattttat ctagaatata ttctaatgcg
     6841 atagtcgaat ctggtaacac aattctttcc gcagaagaat gagatatatc tctctcatgc
     6901 caaagctgca tatcttgaaa tccaacgttt acattggagc ggataacgcg cgctatcccc
     6961 gatattcttt cgcaaattac tggatttctt ttatgtggca tagcagagga gcctttttgt
     7021 cccttcgtaa atggctcttc cacttctctt ccttctgttt tttgtaaaag tcgaatttct
     7081 gtagccattc gatctaaact agaagctgtt aaacctaaaa cagacaaata aaatccatgt
     7141 cgatctcttg taatgacttg cgtagtaatt gggtctacag caagtcctaa tcttgtgcat
     7201 acaaattctt ctatggcagg atcaatgttg gaataagtcc ctactgctcc agagaatttt
     7261 ccaactgaga tttctttttg agcttgtttt agtcgttcta gatttctttg catctcagca
     7321 tagaagagag caaatttcaa accaagtgtc attggctctg cgtgaattcc atgtgagcgt
     7381 cccatacaag gagaattttt ttcttgaatt gctttttctt ttactgtttc gagtaacgtt
     7441 tctactcgtt taatgataat ctcaattgct tgcttcattt gcacacaaag tgcagtatct
     7501 ccaatatcag aagaagtcag cccatggtga acgtggcgag aggctggacc tatataagat
     7561 cctaagttag ttaaaaaagc aatcacatca tgctgaagag tctcttctag ttttaaaatt
     7621 tcctctactt taaattttgc ttttgtttta atatcattaa aatccgcctc aggaatttcc
     7681 cctctatctt ttcttgcttc acaagctagt atttcaatgt ctttccatat ctcaaatttg
     7741 ttttctaaac tccagatctt tgcgatttct ggattgctat atctatcaat catttaaata
     7801 aactctccca atattttatt gtatcaaaac gtgcgtcatc cgcttcagta ggagcccaag
     7861 atgcaaagaa tttatcttct tttggatcat aaggtcctga ctttccttca aaacatacac
     7921 aaacatcact gagtgtaact agtccatgcc aaacaccagg ctgaacatca atccctcgaa
     7981 ttggtccatt gtcggataat acgtatttgc cggtaatatt tccgacatca tcaaagataa
     8041 gaaatccaag acttcccttg agcgctataa atgtttctgg ctttggggga ttttcatgtt
     8101 tatgtggttg cacataagtt cctcgagtga gaacatttaa aaatcgttga tacacctctg
     8161 ttaattcatg aaaattgtga tttgctcgct tgcgtggact gagctttgct ttctcggaaa
     8221 cagaagaaaa caaatcttcc gtgattaatt ggactttttc tttcataatc ttaattctag
     8281 ttttttaaat aagccttcat tggcaattag aaattcatcc atatctatca ttcttttatc
     8341 tctctccttg acgatagtcc ttaggttgat aacattttga attcggatat tacgaatggg
     8401 aagagttcaa attccattct gactacatta cgacgatatc gtaatatagt cagaatagat
     8461 aagataaaaa ttctgaggca gagcagacat taatggatga gttagaagtt tttttccact
     8521 ctatttcttc ttttgtcact tgtatgcaat aagcatcttt tagatgcgga tgaaattttt
     8581 tccaatgagg cgaaatttct ttttcattcc acttagcttc cactgctaaa aaaggatttt
     8641 tatcttttgt aactagaaaa tctatttcct ctttttcttt atttcgaatg aaacacaatt
     8701 caaaatttcc gtaacctcca tccgtccagt aatgacatgc tttgagtaag tgagaggcga
     8761 ttagattttc aaatctagct ggcggggaag gaatttcgct gtagtcccaa agatatactt
     8821 tgccttcttt ttttatagat ctagaaatat ttctttgcca aggttttatt tcgtaaatat
     8881 agtataattc tttgagtgct tctgtccatc tagttactgt gtcaaaactt aattcaagaa
     8941 tttctcgtaa agaagcacga ctaaataaag agcctactct ttccggaatg agtgcggcta
     9001 acatctctaa tttcccaatt tcttgtattc tgctcaaatc gcgtaaatct tctctaagaa
     9061 ctctttcgat tctacttgtt ctccaaagtc ttgcttttct ttcgtcttca gcgaataacg
     9121 gttctggaaa tcccccaaat ttgaataagg cattgaatgc tgttatatga gcttttcgat
     9181 gttttcttac tctatttttt agagatagaa taaactcatc aggggctaat ggtaaatatc
     9241 ccaacatttc tcctaagcta aatggatgca ttctaaagtg ataatatcgt ccaactagac
     9301 tgtcacttcc cctgttgtat acattgagtc ttgcacttcc tgttactaaa atatctaagc
     9361 cggaagcaag agtgtcatat aaacctttta gttctctttt ccataatcta gatttgtgaa
     9421 tttcgtctaa gattaaaatg ggagtatgtt ttttgccaac gggcacaatt gattttggtg
     9481 attttgccca aattcttcta aattcgatat cgtcccaatt gcaatagttt cctattcctc
     9541 tttcttttaa aagcatttgt ccaaaggtag ttttaccgca ttgtcttgga cctgagatga
     9601 aagacatttt attttggcca aacgaaattt cagaaagtaa agtttctatg tatctagaat
     9661 tcatttctga ctagattacg atgaagtcgt aatctagtca atgaataatt ctgactaaat
     9721 ttggataaga tcgtaatcta gtcagaatta attatttcct taaattttta aatatttcca
     9781 tctttcaatt cccgccttaa atcgctcagg agtttctcca aatccaattc gaatccagcc
     9841 ttcttgttca aagttattag cgggtaatac aaatatatcg cattgcttta gtagttcatc
     9901 agaataatat tcagataaaa ctccttcttt caatttagca atagacacta agcctccatc
     9961 tggcttccga aaagattgaa tgaaaggtaa atcgtgtgct gccttataaa atatttcaat
    10021 attttgttga attgaatatt tgattggagt tagtatttcc tttctctttt ttatgatttc
    10081 atatgcgata aattctgaaa ttggatttac agtatgtgtt aagtaatcct taaaggatct
    10141 agctttttct attaattttt tatccgcaac gatccaacca atccttaaac cagtgacccc
    10201 aaaacatttc gtaatcgagc cagttgcaat acatttttca ttcagaagag ctcccgaaaa
    10261 tcctaattca gaattgtagt ccaagaaacg atagtgttca tcaaatatag tataaccttt
    10321 ataatttaag attatttctt ttaaaagatc aatgtcttct ttttgtagtc caatgcccgt
    10381 tggattgtgc ggatgattta gaatagcaag tttaacattg tcttgaaaca attcctttac
    10441 atccaaagaa tcctttacta aaacaggatt caattcagct ccaagcatag aaggtatttc
    10501 atataacgct tgaaaggcgg gagaaaagta tgagacgaca tccccctttt ctatgattaa
    10561 atgaaatatt atataaagtg cctcgcttgt tccactggta atcaaaactt gatcagggct
    10621 cacgttatta tagagactag caatttctgt tcttaaatct tctcttcctc gattcggtga
    10681 atctgctaaa gatattcctc tcataatatc aatcggcaaa tttattttgt caaatatttc
    10741 acccactgta aaatttctaa gcccactctc acctaaatta caacaaacag taagtctaaa
    10801 tttttccaat cgatcttcaa taaagaattc cctgggattc aatttttttc tgtcctattt
    10861 cttccgcttt ttttcgctct ataaagggcg ttatccgctc tagctataaa agattcaagg
    10921 gtatcgtctt cctgatattg tgtaatacca atacttattg ttacaggtcg gtcttctaga
    10981 aatgagtaag tttcaaccag taaccttatt ttttcacaaa tacgaacgcc tgcatcaatg
    11041 tttgaaccaa ctaaaagtaa ggtaaactct tcgccacccc atctacataa tatatctgtc
    11101 atccgaatct gtgctttaat aaactgtgac acacttacaa gaacttcatc tcctttattg
    11161 tgcccataag tgtcattaat tctcttaaaa tgatctatat caaaaattac taaacataat
    11221 gattttggat taaatttatt ttcatcaata gtctgcttga ataattgttg aaatttggaa
    11281 cgattaaaga ccttagtcaa ctcatcgaga cttgcctttt cctctaacgt tgttttttga
    11341 ttttcaaaga gggtaatatc agttagattt agaatgctaa ctcctttttc tgggataata
    11401 ttttgtttta ctctgaaaat taaaaattct cccttaggat ttcttatttt tactttcaac
    11461 tcttcatctt ttggaagctt cgttaagcgt agattagcct ccttgtctat ttcattctct
    11521 aaaagtaaaa agtctaatat gtgcggataa ttcatgttaa atgtatctaa ggaatcaacc
    11581 ctcgaaaaat cgagcaaact ttgatttgct gcaagaactc tttccccatc tgtcataatt
    11641 acaatatctt tttggaaatt caaaagagtt tgcaacattt cctgattaaa ttttaaagac
    11701 aaatgcgtct ttactcttgc caataactct aacgcattaa aaggctttac gatataatct
    11761 acagccccaa gttcaaaacc tttgacaata tcttctgttt gtgtcttggc tgttaaaaaa
    11821 ataattggta tatcctttag atgattaatt tccttaattc ttttgcaagt ttcatatcca
    11881 tccagatcag gcatcattac atctagtaat attaagtctg gctttagttt ttcaagaaca
    11941 gacaatgctt gtaaaccgtt agtagcaaca tttactttat aacctgcgtt ttttaaaata
    12001 gttcctaaaa gctgtatatt ttttggagta tcatcaacaa ttaatattga aacattcttt
    12061 aaattaccca acataatgtg acctcagatt ctgaattaac tcggggtagg ttcttaataa
    12121 tactattagt gaattcatgt caaataaaag tgctttcgct tgtaaatttt cagaatatct
    12181 catcagtgga ggataattat acttttctcc taaatatttt attttgtatg caaattcttc
    12241 tatttcgttg atactagatg catctgataa ctctttccat tcatcataca aatcattttc
    12301 catttcgact agcaaagtct ttaaattctg gataccagca tcatccaatt ccaatatttg
    12361 gtgtctagct tcctcttgat tttcaagatc tatccgaggc ttttcgagga taatatcatt
    12421 tttcccctct aaaatacttt catccgatat tgatacatct tttaaaagaa tttgaaaaat
    12481 actaccctta cctacttcgc ttttgatgga gatacttcca ttcataagac taacgagtcg
    12541 acttgaaatg gctagaccaa gaccagttcc tccatactta gcatgatttt gtcccttctg
    12601 ttgtgtaaat gcctcaaaaa ttgcacgttg ttcatcaatc ggaactccaa ttcctgtatc
    12661 ctcaactgtg aaaattaggt cgatcttggt tgtatcaact acctttgaga ttgctcttgc
    12721 ggttaaccgt atgtagccct tttcagtgaa tttaattgca ttactaagta aatttaaaaa
    12781 tatttgttta aaacgaacct catctagaat tatttcggga ggcagatttt cattaataat
    12841 gatttgaaaa tccagatttt tttcatttat cttttgaata aatatatttt ttaaatcaga
    12901 aaatacttct ttgatattaa cagaagaagt ttgtagttca acttttcccg attcgatttt
    12961 agagagatct aatatatcat taattaaggt taacaatgtt ttgccacttg aaacaatgtt
    13021 ttcaaaatat tttttctgaa tatcatcttt gatggatcct ttcagaatct cagtaaaacc
    13081 gatgatcgca ttcattggag ttcgaatctc atgacttata tttgctaaaa aatcaccttt
    13141 tactttgctt gccgcttcag cagctttctt tgcaatcatt tgttctgtaa tatctagtcc
    13201 aaaaccaatc agaccacctg taaactcatc ttgaaagaaa tagttttgaa aataccattc
    13261 atttccatca ttatataaac cattggaaat aaaaacttgc ggttcccctt gcaatgcatc
    13321 tcttagagta ggacaaaatt cagaatacac ttcaaatata tttatattca cagcttgatt
    13381 atcaaagaga cccattttcc taagtcctgc tcctatgctt tgaataatca atccctctgc
    13441 attcactcta aatacaatta caggcatatt tgctaatatt cctgctaaaa tttggctttt
    13501 ctcattaatt tctctttcaa ttttttttcg ttccgatata ttgcgcataa cggcatgcac
    13561 ttctaaactt tttgtatttt cgttatagac atatctcgat acaatctcag tccaaatagt
    13621 agtcccatct tttttatatt gctctatctc atctatataa atatcattta tatttttctt
    13681 tttagcggtt agtcttttaa ctctcaaagg aaatgctctc ttcgcataga taagagatct
    13741 taatgacaac aaatcgttaa tcggtatact catcatttcc aaaactgtat agcctgttag
    13801 gttttgaact gatgggctta cataaagtag tttcattgtt cttgcatcaa taacaagaat
    13861 aacatccgcc atattctctg tgataattcg atatctctct tcattttcaa ttaaagcttg
    13921 ttccgctaac tttctctctg agatatctct tatattcgtt agaattccaa catttacatt
    13981 ctcattctga taaactgaaa gttgaacttc aataggaaat acagtattat cctttcgaat
    14041 tcccttactc tcatagttat aattagaata atcagaactt aataatgatt tcattatcga
    14101 agctattttt cctctttcat cctgttcaat aaaagctaca gcatttaact ccctaatttc
    14161 atctatcgat ccataaccga acattctaag catattgtta tttgcccaaa gaactgaaaa
    14221 gtctagacct attaataaaa atgaatcagg tgaaatttct atcaatgttc taaaaagttc
    14281 ttcacttcgc ctaacttctt tttcaatacg acttttctcc gcatcaatct ttcgatgctc
    14341 ttctaccatt tcattaaacg ttttaataaa attccctagt tcatcattgg agtcatattc
    14401 tatttttact ttccaatccc cctttgcgat cctatttgca gctgcttctg ctaattgaag
    14461 tggaaaaaca aattgtttaa atgtataatt aattaccaaa taaataaaga cgacagtaaa
    14521 aaatgaggtt aatatcatct gaaagataag tgaataatag gatttatcaa caatctgatt
    14581 ggattctaac tcaccacgtt gatttaatga taacaattca tataaatctg aatttaattg
    14641 atctactatc gttttagatt tttcttgcaa gctacgaatt gaagccttat taaactttct
    14701 agactcttca aggatttggt tatgaatctt taaatattct tctagatcgt ctctaaattt
    14761 agcaaaagat ttctcttctt cgtcatgata atttagaaca cgataataag ttttacttcc
    14821 cactaaatcg atttgcaaat ttgagattct cttttcaatc atctcttttt ctgatatctt
    14881 atccgctgca acatgtttgt tttcgaattt ataaatctga tcaatttgat atcgcatttc
    14941 agagatttga ataatacttt gcatccattt attgtgaatc tcttttgtct cttcatgtat
    15001 cttatttact aaatgaatag ataaagaaat accaaccaat atcaaaaagc aaattgcggt
    15061 aattccgact aaagccttct tttttatctt taattgatta taccaactaa ctttcgtaat
    15121 gtagtatttt gtgacagaca ttatcgattt aatactctac taatgatttg attgagcgaa
    15181 tctggatgaa acttatccat tataaaatcg tccgcaccag attcataaat aacagattta
    15241 acaagttggg aactatcgga tgttatagca acaatgtagg tatcatccac tgataagttt
    15301 tgttcgactc tcagtttttc aataagatct ttcgcattca tatcaaccat atggatattc
    15361 ataaataata ttttaggctt aatattgcta caaattgaaa ttgcttcaag agcagtcttt
    15421 gtagtaataa catccagctt ttcttttttt aaagcttttt caagtcgaat tgtaaattct
    15481 tcatctcgac atatgatgag agcaatttcg ggcttattgc tatttgcctc aacagtctca
    15541 actgaatcag atagagtttt atctctacag cgattgatat acagagcaag aattggatct
    15601 tctggtaata ttgttttaca tttagtaaat atctcttccg ctttgtcaaa aaatccacct
    15661 cggaataaag ttaatcctct aaaatattca gcattaaaat tcttcttctt tttcagcata
    15721 tcttctgtat cagcaccaaa gtattcgtat acagttaaaa aaccctctgt cccttgaatc
    15781 tttaaatatt caatctcacg acagagttct ctttcttttt tttcgagttg taaataagta
    15841 tccgatgata ttaaaacatt tacaaagaaa gagttggcta gtgtttctag tttttgtgcc
    15901 atctctacag gattaccaat tactgttgta tccattcgat caattcctcc taacgttcca
    15961 aatgtaacat ttcctgaatg aataccgaca cctatattaa atgtagattt acctagttcc
    16021 ttgtttctta aattaaaaga atgagtatat tgattcatct ccgtagctgc aagaattgca
    16081 ttttgtggaa aatctggaaa caaaaccata atttcatctc ctataaattt tccgatgaat
    16141 cctctttttt tgcgaataat agccgttatc ccaatataat actcatttaa taattcgaat
    16201 atctccttag ggctgagtct ttcactaatt gcagaaaatc cttttatacc acttactaat
    16261 atagtgacat ccctctgggc taaatttccc aatgatactt ctgtaatact ttccttattt
    16321 aatatagata gaaattctct tggaacaaaa ttgctatagg ctgcattcac taaggtgagg
    16381 tggtttgaca aatcttcaat gtctctaaat gctctagaaa atcttctcga taaaattaca
    16441 gattgagcaa gtaaaaaaat aaaaacacct atttgcatca attcaccagt aacgatgact
    16501 tttaaattat ataatatgtc attcacaatg aacaagatta aaaaattgga aaataaaaat
    16561 attacaagag catcctccct tctcttaaca actaatctga cttgatatat atagattata
    16621 acaataatcg taataatgac tacataattt ggatttacaa aataattata gagtcgcaaa
    16681 ctagaaaagc aaactatcat gcttaagact cccccaatag acatactgat ttttacaaga
    16741 agatctgaaa cgtcatctgg aaataactga taaaaaaatt taaagaatat tgaaatcaaa
    16801 agaaagaatg acatgtattc aaattttgtg acaacctcat agtggatatt cggtattagc
    16861 tgcacaatca taatctcacc agtagaaata acgcgcatga gagtcacaag gcagaaacta
    16921 gcgaaataaa gactagttaa atcattcttt ctaaatataa agagcatgaa gtgatagatt
    16981 gtcatgatga gtatactacc acctaagaga aactctatag aatttctttc gtattttaat
    17041 tgaagaattt gatcttgggt tcctaagata ggaatactcc atattcctcc attgcgatga
    17101 tgataattgg aaacgataag cgttaactca aagctttgct gattcgtttc aaaaaaaatg
    17161 gtttctgttc cataccctgg tgtagactct ttagggtttg tgcctatccg accacgtcct
    17221 gttaataatt cattgcctaa atacagttca taagcggtag cctgttcttt cattctaagt
    17281 gctaatttct tttttggaat agaatgcata ctctctggca gcataacttt cactttaaaa
    17341 gtagcatatc catctccact taaaatttcc ttatctacaa ttctaccatt ccaatatcca
    17401 ggaaattcaa agtatccaga caataactcc tttgaaattt cagtttgaga aggacgatag
    17461 atttttttcc aataaaactc ccactccccc tctaaattca ctgcgccttg cttttcaaaa
    17521 tcccaaaagg tcagatttaa aactcctgct tccacttttg gtgagtcttt tagagcaaga
    17581 ccgatattac atccgttcaa tagtaaaaga gaaataaata agaaaaaacc tttggctaaa
    17641 agcatatgca atgtttatag gaaccaatga aaaaagaaat ctcttttact ggactttttg
    17701 gcgaaaaaat aaatgttggg gttatacaca acggacacga acaaaacaac ctatgaaatt
    17761 acttatgatt caacgattat tttttttagc aattccaatt agctttattc tttattgcac
    17821 aaccgcaaag ccagtagata tcaaaaaaca aaatcccaaa ttatacagag agttaattcg
    17881 cgactattca gttgtgagtg aagagaatcc gggtaaaatt gaaacattaa aatttatcga
    17941 aaatgaaact caatcgattg taggaaaaat aaacaatttc cctatcgaat cctgcacttg
    18001 gttctttgat cgaaaagtaa aagataaaaa caatctagat gatactatgt ttctacagaa
    18061 agcaattaac ctaaattgtg gtggtcaata ctatataatc gaactagaca aacaaaacat
    18121 tcaggcaaac aaactaaaat accaaggagt ttataaaggt atttttgatc aactccgcaa
    18181 agcaattatc gtcgaagcat cctgcagcat ttattccaca acaaacaaag aggaagatga
    18241 taaaaataca gctattgaat gttaccctta cccttatgat ttagagcgtt atacgaaaaa
    18301 tcaaaagaga gaagttgtag cgggatactt tctctacaca gaaaatcaag aaaccttgga
    18361 aagtctcatg atggcatcca aggaagaaaa ggataacaca aaagttttac aagagttaaa
    18421 aaaagatttt gtaagtgaga cagatgcaaa caatcaaaag acaaatcctt caattggaaa
    18481 gaatttcact tttaaaaatt gcgaatatat tgatttcaga gaagcgcaaa ctttgaataa
    18541 gaaaggaaaa gatttaaaag caaaaattga taaatttgta gaatctgacc ttactgatcc
    18601 cacaaataaa gaaagcctaa gagatgcaat agaggaatat agaacttgcg gaaaccgttg
    18661 ctatagcaag gacaaagaaa ttcaaattct atttgaaatt aaaaatgagg aagccaaaag
    18721 aaaaacgctt attattaaaa agctatcctc tagatctgaa ctctcaaaat ataaacgttt
    18781 taatttttat cccgttgaag gaagcctaaa atccattgat ttttctgtga aaggggatat
    18841 agaaaaaata tacttagatc ctatcataga aacgactcca acccaatctg acataaaacc
    18901 agaaccttct aaagtaatag aggaagtaaa aaaagcagaa gaggaagaaa aaaccaaaga
    18961 gaaaaaaaag aaaaagtcta aaataacaga aacggtagtt cagcccattc cagagcctga
    19021 tgcagaggga aacacggaat ctgttcttcc aaaacaagag taatttttcg aatcggttca
    19081 tttttgcgtt tttactcggt ctaattttac cgaaaataaa aaagatgctt gagtgttttt
    19141 tcgattggct tacaaaatat actccaagtt ttattttttt taaataaagg tgctaaatga
    19201 gtagtaaatt aaaagctgcg atgtttgagg aagaatcagc tcttgaaaaa ttagaaaaag
    19261 agcaggtaga agtcataggc gatacaattg acgaaagttt acgacttgcg gcaaaacact
    19321 tcgggaaaga actgtatcaa ttagattatg aagttatcga gcgagggaaa aaaagtctct
    19381 ttttctctca cccctacaga attcgagttt ttatttctaa tcatgaagac aatatacaag
    19441 aattagctgg aatcgataaa aagcttaccg gtggaagtgg caagctctta tctacagaat
    19501 taaagaaact aattgttcca aaagacaaag atggttgggt cagcatcaaa tactatcgtc
    19561 atggacttta tatgaaagaa atcccacctg tgggaagtgg aaagtatcca acagtccaag
    19621 acattataaa aaaattaaat ttaaggggaa tcgctcctcc aaatgaagca gctatccaaa
    19681 gctttttaga ttctaaatca acagaatacg ttcgatttgg agatgcaaaa cttaaaccgt
    19741 ttgcagaagc cggagctaaa gttgaaatct ctgatgataa tatgaaggca tttgttacac
    19801 tcctacctcc aaaactagca ggaagagact tggaaatagc ggatgtcgtt tatgaattaa
    19861 agaaagaaga tattgtttat ggaatcaagg aagacgttat tcgcgagttc cttgaaaatg
    19921 aaagatacaa tgaacctttt gtagccgctg caggtgatcc acctattcat ggaaaaaatg
    19981 ctcagatcat ttatcatgtt agaactgaaa agaaagttgt attaaaagaa gatgcaaacg
    20041 gtaaagttga ctacaaagat ttagatttaa ttgagaacgt tgtagttggg caattattag
    20101 cagaaaaaac tccaccagaa aaaggaaaat acggtcgcaa tttatttaat gagatgttgg
    20161 aagcgaaaga tggtcaagac attcctctca atcaaggcaa aggaacaatt ctttcagaag
    20221 acaagatgaa attaactgct gaagtaaatg ggcaagttat ttttgcagaa gatcgcctat
    20281 cggtcgagac agtttacaga atcagtggcg atgtgaatat gagaacagga aatattacat
    20341 tcctaggttc cattgtgatc actggaaata ttgaagataa tttccaagtt aaagcatcag
    20401 gtaatatcga aatctatgga actgtgcaaa aggcacaagt agaagcagac ggtgatatta
    20461 tcattcgcca aggcgtaaca ggtagaggag aagcaagaat tgaatctacc ggcggaaacg
    20521 ttgtttctaa atttctacag gatgcgatag ttgtcacaga taaggatgta gtagttcaag
    20581 aagtaataat gaactgtgat gtgagcgcag gaggaaaagt aacctgcaat gggaaaaaag
    20641 caaatatact tggtggaaga atcagagcgg gcactcttat ttctgctaaa aatattggat
    20701 ctccagcaaa cccatacaca gaactcatag tcggagtaaa tccaaaatta ttaaagcaga
    20761 tagaagacta tacagctaaa agaaaagaat cacatgataa actagaactt ttaagcaaaa
    20821 catacaagac tatgaaagca agacaagatg atgatccatc ttcttttagt gaggaaaatg
    20881 ccgcttattt agctaaacta gaagtgggta ttaaaaagct agaaaaaaga atcgcagaat
    20941 atgacaagga aattcagact gtaaattcat acatggaaca agctggagac gaaggaaaag
    21001 tcttctttga aaagaaaatg tatggtggag tatcagtgcg aattaaaaac gcagagcctt
    21061 acaaaattaa aaatgattta caatcaaaaa ttttatttct atcagagaat aaaattcaaa
    21121 tgaaaccttt tgctgatcca gaggcagaag gcggacagtc taaacgaggc ggaagaagaa
    21181 agtaggcaag gagatttata gttggaactt ttagcattag tcattatcaa tatagtcttt
    21241 ggcgtaatct tatattatgc tgtctctatt aaagtgacta atagtgttag agactaccaa
    21301 atagtaaaac tcaaaaaaga aatccaagct catacactta cattcttcaa agaatctgaa
    21361 agttatcttg ctttaatgga ttcacgtata gcagtattaa aaaatctttt acataaagcg
    21421 gaacagatgg gaatcgattt taaaaatctc ccagaacaag aaaagttagc cgccagctta
    21481 agcactacca atactgaaag taatcaaaat aaaaaaccat atgtatctaa atttgatgaa
    21541 gaggcaatac aggattatcc acaaaaaacc gttaagctaa aaccacaatc aagtgttata
    21601 aaaaagacag aagaagagga caatggattt tttagtggtc taatcggagg tctaggaaaa
    21661 ggcttaaaat ctatatttgg aattaatagc gatatcatga ttgatccaaa tctagataca
    21721 aatacaaaga ctaagaaaaa ttctgtgatt gatgtttcca ttggtggaaa tccattagaa
    21781 gcaaccgaat ccgttcagga caatctcaaa acagaagaat cttttcaaac agtatttaat
    21841 acagtcaaca atccctatat tcaaccgaaa caagaagata cattacgcat ttccattaaa
    21901 gccgctttag aagaattacc agctgatgca acgaaagttg aaaaagttgt tcatttatta
    21961 aaaaaaggtt attctcatac agatatttct gaagaaatgg gactcgcaat tccagaaatt
    22021 tcactcatcg aaacaataaa aatagaaagg tctcgaagaa tctgaaatgt ccgcttttaa
    22081 aattctgaat ataggttttt caaatatttt aatgacttct cgtgttgttg cgatcatcca
    22141 tgcagattct gcttctgcaa gacgcctacg ttccgaagca aaaaattctg gccacttggt
    22201 tgatgcaact aacggaagaa aaacaagatc cgttatacta accgatacaa atcatcttgt
    22261 tctatctagt ctcaaagtag attctctggc aaagagaatt gaggctgatg ataatagcct
    22321 acctgcattg gaggaggaat cccttgaaag tgagccctag aggaatatta tatgttatat
    22381 cctctgtcgc tggtggaggc aaatcaacat taatctcttt attactgaat aaatatcctg
    22441 atattcattt ttcaatctca gtaacatcta gacctgttcg acccggagat gagcctggaa
    22501 agaattatca ttttgtaagc agggacgaat tcgaaagatt aattgctcaa gactttttct
    22561 tagaatgggc actcgtgcat gataattatt atggaacttc aaaacgttat atcgaaaaag
    22621 aactacgaaa agggtataaa gtaattcttg atattgatgt gcaaggtttt agaattatca
    22681 aatccaaaat tcaaaatata aaatcaatct tcatacttcc cccatcagaa gaagtttgga
    22741 ttgaccgact taaaaaacgc ggttcggata gtccagaaag tatagaaaga agaattcaaa
    22801 atgggcgtaa agaattgcaa caagccggcg attatgacta ccagattgta aatgataaat
    22861 tagaagattc ctttgccgaa ttagagaaaa ttttatttgg cgacaaacca aaggaagatt
    22921 ctcctcaaca tgaataattc tctttccgtc caatccggca tattaaaagg gaaaaaaatt
    22981 ccaataccat caaccgttaa gggaaatagt aattttactt cagctatact aaaaaaatca
    23041 gtattttcta ttcttgattc acttcatctt caaaatttaa tcaatttaga agagtccttt
    23101 tttatagatt tgtttgcagg atctggtcaa atgggaatag aagccgttag tcggggattt
    23161 aaaaactctg ttttctttga gctagataaa aatcgtttta gaggtttgaa agatttttta
    23221 ccaactataa aaaaagattt tattctatta cacagagact catttcgcta ttatgactca
    23281 tttgataacg agccagtcaa atcactcgtt ttctatattg atcctcccta ctccttctgg
    23341 gatttgaatc tagagaaaat cttgcgctta acgaatgata ttctaaacag ttacactcaa
    23401 aagaatattg ttttattagt ccaatcccca tttgacccaa aatggaacag ttttgagata
    23461 agacaggtag gaaataatta tctattatct ctcgtaaaca aaattctttg atacatgaaa
    23521 aaatgaatac aggtctaatc attgcgtcac tttcttatca agaaaattaa tttaattttt
    23581 tatctcttca tcttattgcc ttttatttta tttgttacaa atatttgcct atctaaaaaa
    23641 aaccagcctc atccagtgtc tttttatttt tggaaaactc aactcaatct atcagaatat
    23701 gaattggaaa cattgaaaag tttaaaaata gagaagatat accttcgtct atttgatgta
    23761 gatagagtgg gtaatatcaa tttacctgtc ggagaagtta ctatcataaa aactccaata
    23821 aatatcttaa tcattccagt catttatatt acaaatagaa gtttagtagg attagaaaaa
    23881 gattccattc agaaattggc agtaaatgtt ctaaaaaaaa cagaaaaaat aatggataac
    23941 aatcatttgc ctttaaaggc aatccaattt gattgtgact ggacggattc tacacggaat
    24001 aattattttt attttttaaa ggaaattcaa aatatttcta aaaataaaat tgctctatcc
    24061 actacgattc gacttcatca aattaaatat agagaaaaaa ctggaattcc tcccgttcag
    24121 gaaggatttt taatgtttta taacatggga caaatcaatg aggcttccac tcgaaattca
    24181 atttttaata aagaagatgc ttccaaatat ttatctcgtt tggagaacta cccaattcca
    24241 ctctcctata ttttgccaat tttcagttgg gttgtccatt ttagagatgg tagaatttta
    24301 gatttaatca caaaaaaaga tatggatagt attttaaatt ctactaatct tgaaaaaact
    24361 aaatcagatg acttgttttt agttaaagag tccttcctat cagaaggcag attttttaaa
    24421 gtaggagatc ttttgaaaaa ggaaactatt tcatctatcg atttaataga agcagctgaa
    24481 ttattaaaaa attcaaataa acagaataac tatgatatta tattttttga tctagacgaa
    24541 atcaatctta aaaggataac gaatgaaact ctcaacaaaa ctatttctat ttttaattag
    24601 ctttttactt acatctatct caattttaat cgcctgcggt ccttatggtt ggatggattt
    24661 ttcatataac actctcttga cacccgaaat cattcaaaat ccaaaagcag aaaagttttt
    24721 ttggtcatat aaatactctc tttataaaag atttaaggat aattataata cagattttaa
    24781 ttctactaac gtttcagaat ggtcaaattt cttcccttcc aaagttcatg aagataaaat
    24841 aaaattttgg ctctactcat ccaatttaaa agaaattgat gaaatgattt ttgctttaca
    24901 aggaaaagga acagtcgcca cagaaaatgt aaagaaattc tccctcatag gttcagctac
    24961 tccaaattct tgtatcgaat ttctctacta tgttggattt gcaagacgaa atgaagagtt
    25021 tactacaaat gtcattgatc tatatagttg ggatgaacct aaaaaaacaa aagacttatc
    25081 tcaacttcaa aagcaaattg atggaggtct tcgctttttc gaaaaagaaa aaaatgattt
    25141 tttaaaggaa agatttatct tccaactcat tcgattatat tactttaact cgcaatacga
    25201 ggaggcaatt tccttttaca aaaaaaatga agctttcttt aaaacaaaca attcaatgaa
    25261 agcaagaagt cttggttatg ttgcggcaag tcatattaaa ttagatcaaa taggacaggc
    25321 aaattatctc tatgcctact tatttgccaa tgaacctatt atgcaagaaa cagcttatct
    25381 ctcctttcgc ccacaaagtg gaaaagattg ggaggaaagt ttaaaactag caaaaacaaa
    25441 acaagaaaaa acagcacttt ggttcttgtt cggaattacc tttgatcccg aaactgggat
    25501 tcaggaaatt tataagatag atcctcaatc cgattattta gaagtattgc tagtaaaaga
    25561 aataaaccat ctagaggaac atttcctact tgtccaacca agtctaccgt tagtcggaat
    25621 caatatcgag gagagtcatc agttttattc tagaaaaaaa agttttctaa aaactgtaac
    25681 tgagattgcg aaggatgata aaatcaaaaa taaatttgtt tggaacatct cctctgctta
    25741 cctcaactat acctatagca atttttcaga aggagataat tatttagcaa aagcaaagac
    25801 atttatcgat aaagacgtaa aaaataaatt actttataac tctcaatttt caattgtatt
    25861 tgcctatggg aaattactca gtaactctaa attaactcca agtgttgagg cagaaatcct
    25921 accgcatcta aaaactattt tcagtaagca gattgaatca attcctaatt ttagatatga
    25981 ctatcctaga ttttggatca gtaatgcaat agccgtccga tatgcgtcac aaggcgaata
    26041 tgaaaaagca gaagtagtgc aaaatggcac tcttaggtta catttttcta aattagaaaa
    26101 tacaaaaaaa atgatttcct actttgacag aaaagatcat tccgaattag agaaatttta
    26161 ccaagcaaga tcatccttaa agaaaaagca ttatcaacaa cttttaggta ttcactatgc
    26221 actccgagat gaactagaat tatctctcga aacatttaaa tccattccaa actataaata
    26281 cacatttcaa acagatccat ttgcgattcg aaaaaaagat tgttatgaat gcgatattct
    26341 ccctaagtcc gctaaaaaat atgatagtat ttattttata caaaaactca tagacttaaa
    26401 aaaggaggcc gtgcaaaata aaaataaaca agcagagaat tattttttag ttgcaaatgc
    26461 attctataat atgagtttct ttggaacgag taaattttac aggaatgatg tccatagctt
    26521 taaacaaaat tatgagatgt ggcaatattc aaaatttagt aaaatggatt tccctgaaat
    26581 ggataatagc actgcactaa aatattattt actcgcagca agtgcaagta acgatccaga
    26641 gttcaaagca aagatgaatt tctttgccgc aaaggcagaa ttaaacgaat ggcatgtgca
    26701 aaacaaaaac tatgataaac attttcaatc aggactttat tactctattt tgaaaaagaa
    26761 tttttctaat actagatatt attctgaaat catcaaagaa tgcatttatt ttgcaagata
    26821 tatcaatgga aaataagaga gaaacttacc accgataggg atgattctat ccgataaaag
    26881 atacgataat ttaaaattat ccatcttatc ttcaccgttc agttatcggt gttcatccgt
    26941 ggtgaaaatt tttttaaatc aatttctttt ccaaaaatcg aaatgaaata gtttaaatat
    27001 ctaaacgaat tggttcccct ggcttcgttt tattaaaatc tctaacctta aaagtgagat
    27061 gactccacgc atccacagaa taatcatctt ttacatggag ggctggtctc atcgcaatcg
    27121 gtttttgttt ctcaggatgt aaataaagtg atcttctacc aaagaatcta cctttaaatt
    27181 cttcttctgt taccttttta attttttccc ctttcatctg aaacgcaaaa ttacaattat
    27241 ccgctaattg tttcatttct tccggattaa ttgatataga atgatcagga cctggtaaag
    27301 atctatctaa tgtaaaatgc ttttcgatta cacaggcttc atatcctatc gcaacagcag
    27361 cacccaatgc accagcagag tgatcagaaa aaccaattgg gaaatcatac atactcgaat
    27421 aaagttgaat tgtttttaaa tttaattttt caggaggagt tggatataga gagacacaat
    27481 gcataagaca aagttcggaa acatttttgg tttcaagaaa ttcgaccgct ctagtaactt
    27541 catacaaatc tgctgcaccc gtagagacaa acaatggtaa accactcgaa gcacattttt
    27601 ctaacaactc tgaatttaca atatcaccgg atgcaatttt taaaactggc actttaagat
    27661 ttaataataa atcaacactt gtcacacaaa gcggagttga gaaaaagatt aacccttcat
    27721 tcaaagcagt tttctgaaac tctctgtgaa aatgctctga aagttcatat tgcttaaaaa
    27781 tatcaaataa gaattttgca tctgaacttc tcggatcaat aaattcttcc gtaacatagg
    27841 attggaattt taccgcatcc gctccagctt tttttgcagc cgcaattgtt tttttaccga
    27901 tttctaaatc gttattgtgg ttcaatccaa tctctgcaac taggaaaggt ttatgatctt
    27961 ttgatataac tcggtctgtt agttgaattt cttttttaaa tctcatcaat gtattcatcg
    28021 gaagatttgg attgaataga aatatttttc atcttgcttt ttgattttga acttagaaat
    28081 ttgaatcatg aaagtcattt ttataattat ttttctattt atctcatgtg cgagcacaag
    28141 gtatataaat cctgaggaat cagagcccac ccgagaatgg ggtccaaccg aaataagtgc
    28201 aacggttgat acaatggttg tctcagtcca aaactatttc gaaacaacta aagagaagcc
    28261 atatattgaa ttgaataaaa tacaaaataa aacttcagaa catattgata ctggaatgct
    28321 tgcgaatgaa atatctacaa atctcataaa aaggaaaatc gttttcgttg atagaagcca
    28381 tagaggagat gcactaaagg aaatggaatt gggacaaaga gggatagtga gtgcaaactc
    28441 tgcattacct acaggagaat ttctttcacc taacttcaaa ttaaatggag aaataacaga
    28501 taacgttcgc tatgtgaatg aagaaaaagt gcaatatata gttgttacac ttcgtctttt
    28561 gaagctttct accggaggcg tcgtctggca agatgagaaa aagtttttaa aagtatcaac
    28621 caaccaaaaa ttcggttggt aatttataga atgacaaata ttggtagaac aaagaaatag
    28681 agcctatgcc cgcgacagta ttaccagata cagatataga taccgagatt aaagaaggaa
    28741 atcgaggtgg accatggaaa gtggttctat gggatgatga tgaacatacc tacgattatg
    28801 taatagaaat gctaatggaa atttgtcaaa tgtctataga aaaagcattt catcatgcac
    28861 tagaggtgga tacagaaaag aaaaccattg ttttcgaggg ggaactagaa catgcagaac
    28921 atgtccaaga cagaatccat gcctatggac ccgatccaag aatggctgaa tccaaaggtc
    28981 cgatgagtgc tacattagaa caataaaaat agattgcact taaactataa atatttttct
    29041 tttcaactta agacttaaat ccataagata gtttaagtcc aattcaacca aagaagaaga
    29101 gtcatgatta ataaaaagaa agttatcgta gttctaccag cttataaagc tgccctaacc
    29161 cttgaaaaaa cctataaaga aattccattt gatattgtag atgatgtgat cctagtagac
    29221 gatgcaagtc cagataatac tcccgaagtt gcaaagaaaa ttggtatcaa acatgtaatc
    29281 gttcatgaaa aaaataaagg gtatggtgga aatcaaaaga cctgttataa cactgctcta
    29341 aaattaggag ctgatattgt gattatgctt caccctgatt accaatacac acctatgctg
    29401 atcactgcaa tggcaagtat catttctcta ggtctttacc ctgtagtgtt tggttctaga
    29461 attttaggaa ctggtgccct taaaggtggg atgcctcttt ataaatactt tttcaatcga
    29521 tgcttaactc tttttcaaaa tattttactt ggacaaaaat tatctgaata ccataccggc
    29581 tatcgtgctt tttctagaga agtattactc aaaacaaatt atgaagtaaa ctcggatgat
    29641 tttgtttttg acaatgaaat gataggacaa attttctatg aaggctttga aattggagaa
    29701 gttacctgtc cgacaaaata ttttcctgaa gcatcctcca ttaacttcaa acgaagcgtg
    29761 aaatatggac taggagtttt aagcgttagt ctcaagttca gaattcaaaa aatgggtttg
    29821 tttaaattta aaatttttga aaaaaaacca gcttagtatt ttacctggtg aattaaatta
    29881 cttcatcagg tagcaacaaa taaaataata aaagagacac tatcagaaag actgtgaaat
    29941 aaatttttcc gatttttttc cagtcgcttc tttcaagaga tagaatcatt ttttttattt
    30001 catccaaatc aaaacttaat tgttcaggtt tataattttt tccatatagc tctattagaa
    30061 aattctgata aatttcgttg cgtttactat tgattaacca ataggactct ttaacaatct
    30121 cctcatcgtc cgaaaataaa ttctgaaaaa ttgttggaac tttataatat tcataaata
//
LOCUS       DAOQOS010000066        29139 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_724_length_29139_cov_6.226860, whole genome shotgun sequence.
ACCESSION   DAOQOS010000066 DAOQOS010000000
VERSION     DAOQOS010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 29139)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29139)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29139
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_724_length_29139_cov_6.226860"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..362)
                     /locus_tag="PLF66_14850"
     CDS             complement(<1..362)
                     /locus_tag="PLF66_14850"
                     /inference="COORDINATES: protein motif:HMM:NF026192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HMZ65373.1"
                     /translation="MDSNKMHTPMSGAKKAALLLLSLGKEEAAKVLSHLDDKMIEQVV
                     YEMSQIRMVSKIQREAILNEFKDTVAELQLGQKGGIETARELLSKTVGFNKADEIIKK
                     IDKKEIAQDFEFLNDIEPV"
     gene            462..1670
                     /locus_tag="PLF66_14855"
     CDS             462..1670
                     /locus_tag="PLF66_14855"
                     /inference="COORDINATES: protein motif:HMM:NF014686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HMZ65374.1"
                     /translation="MYNSGYYISIGAGKNQLPLIHKAKQLGLKVIAVDQNKDAVGFKY
                     SEIKILHSILDFRKIYATVLKLFLEEPIIGIGVRSYGKAVLTASYLAEKFNLINTSLD
                     ILRPFYNKKTMKAFLGQNGIPVPTSYIWRYQKDFYDLVSKVSYPCILKPVDNIAKLGI
                     EIFDNKEELLNRLTQLKARNEKFLLEEFINGKEITVLGFVQNQKFTLVSISDKITTDF
                     PPYLELSHQLPSKQEQHLGEVHLLCQSIVNLTNLDNSPFVAEFKISDNGKIYLIEVMP
                     EIGGEYLADLLIPEFYQYDYFKNYIKMTLGERIESVDSQKIKKKNSIGQICFIAPPEG
                     SSKFIQSNNVSIKGDRIFLNEKLKDYNSILNTKEGNSARVGVIGVISSSKTPEELDNE
                     IRKNFNAKFE"
     gene            1654..2280
                     /locus_tag="PLF66_14860"
     CDS             1654..2280
                     /locus_tag="PLF66_14860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017214427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HMZ65375.1"
                     /translation="MQNLNKINPTIWDNHYTKEKSKLVFPDENLVRILSRIPVGTSAL
                     DHGAGSGRHSVLLKSYGYQVTACDYSAKAIEGIQKMLPTITTNVITSTTLPYEDKSFD
                     LVVSWGVLHYNSFDEANTIISEIKRILKPNGYIVGTVRSNLDTHLKTKDGVIGNKDLS
                     GGKVTLYSLDELKDMLKIFKDTEFGYMERTPMGKLEERICHWIFQAKN"
     gene            2256..3134
                     /locus_tag="PLF66_14865"
     CDS             2256..3134
                     /locus_tag="PLF66_14865"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000347674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HMZ65376.1"
                     /translation="MDISGEELKKNCPLDKECDWCFLYRSTYNYYDLPIYKCNTCGIQ
                     TIHPKDKYDLKAMYNSNYYSGNASYYYHDERKNEVYESFVWDARIRNIKKFIPKGNFL
                     DIGSSFGGFLNRAKMAGFETFGVEISEYSAQYANERGIRTYNGSLVEADFPAEFFDVI
                     TLVEVIEHLEKPDIVFPKLFKILKPGGLLLIQTANFDGMQAIQEGPKYHYYLPGHLYY
                     YSLSNLKKILTKNGFKDFTVYHGVDFPLYAKLFKSMGSFRKWTDYFKWFRISYYHLKS
                     KFFKGSTSSMVLYAFK"
     gene            3144..5495
                     /locus_tag="PLF66_14870"
     CDS             3144..5495
                     /locus_tag="PLF66_14870"
                     /inference="COORDINATES: protein motif:HMM:NF015587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjD/YihY/BrkB family envelope integrity
                     protein"
                     /protein_id="HMZ65377.1"
                     /translation="MAQTGLEKIKISDKFYLIPEKKGFKRSYIIFLRILLVSLHRFLA
                     DDCLTRASAIAYTNIVSLVPVLTVALALLTISSGFTEKQDELFDSINTFLQKNEIRID
                     ITPYLETLKDIINSATQIGAIGFIVLIFSATAILRTFEAAFNQIWRITTPRPFADKII
                     FYFFILSIGPLLGAIFTSFAARFADATRASHLYSITRSSDNFLWMAGENGTIVKIQEN
                     GKRLAKLKDFKIDYENMYCMNLDKDAEANCDIPKLNKESFVKIRNRKNQLVTISENGV
                     ILQSFDLGQTWSIAEIVNAKVLDFTIADENNMFIVLTDGEIINYHNKDKFDKVFVNYS
                     NSEDKVIATKIRFPDPEHGYFLDANGYFWKTSDGGKTFQSIKVTKGTLNLTDLAILDN
                     ENYAVVGERGAIYRTHDAGKTWIDISHKRVSYTKIWYMQNEGKDELIVLNDLGSILYS
                     NDLGNKWQLTYTPTNGKLHAMIPINPKFGFTAMNPEDESVPTEEEIAKQKEKQTVGDI
                     LAVGEFGKISIGDVKDGTLEWHTIAGGDNIFSLYSFIKTVIPLSAIWIFFMMLYTLIP
                     NTKVPLKASAIGAALTGFILLSFFWAFGIYLRSFATSTMVIYRALAAVPIFLLVVYCF
                     SIIVLFGAEITATLHYKDRYNSNRHPFDDGEQDIKYTYYNSIKYLVLLYENQSKNKAF
                     LTVNSLKTSLNLSTLDFDALTEKLVSDKFIVKVEGGQIAPCSMPSDLTLFDLYDKISH
                     ESYDLPSFADQKGFVIKISEQLKEVNNFSKQTLSKIKFSDLLV"
     gene            5506..7320
                     /locus_tag="PLF66_14875"
     CDS             5506..7320
                     /locus_tag="PLF66_14875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016546861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HMZ65378.1"
                     /translation="MIKYDYDYIVVGSGFGGSVSAMRLSQKGYKVAVIESGKRWKSDD
                     FPKTSFSLSKYVWFPKIFCYGIQRINLLNDVMILSGAGVGGGSLVYANTLYVPPQPFF
                     ETPIVQKMGAEKGLLPFYDIAKRMLGVIENPKLWEVDDYMKQTADEMGVGHTFKRTPV
                     GINFHEKDKVDPYFNGEGPERNSCNFCGGCMVGCRFNAKNTLDKNYLYFAEKLGATII
                     PETKVDKLIPLSPDGGDGYEVQTRSTTGFFGNPRKTFRARNVVLSAGVLGTLGILLKM
                     QQEKIMTRLSPMLGKTVRTNSESLVGVTSLRKGVDFSKGIAITSSIYPDEHTHIEPVR
                     YSAGSDAMGTIAAGTLVDGGKGVPRFLRFLWSCVQHPINTIRFLSPFGFAKKSIILLV
                     MQTVDNSIRIVRKRRLIWPFQKTITSTQETGTHIPTFIPIANEFARRLAKRIGGVARS
                     SLNEVILDIPTTAHILSGCIIGDSPETGVIDLKNQVYGYKNLLICDGSMVPANLGVNP
                     SLTITALSERAMSFVPLASEVKEHRFFDYEKNWKITDALTGKEIKSSETQPKDAEKKE
                     NSKSISKPKVSNKDTKAAKPAPAKKKATQVKAKKKIKK"
     gene            7511..8035
                     /locus_tag="PLF66_14880"
     CDS             7511..8035
                     /locus_tag="PLF66_14880"
                     /inference="COORDINATES: protein motif:HMM:NF014543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65379.1"
                     /translation="MNFKTYFFVLLLLSLPLAAQGNIKCIDGDCVNGNGITVDDAGIR
                     YEGSFQNGQKNGKGTQIEPNGNIYKGDWKNNLPNGKGTYTLRSGETRKYTGSFKDGYY
                     NGVGTLQYANGDKYIGEFKNDKRSGYGTFISKAERKIYIGKWKSDKQNGKGIYYAYTS
                     TEKVKFNKGSLVLR"
     gene            complement(8028..9026)
                     /locus_tag="PLF66_14885"
     CDS             complement(8028..9026)
                     /locus_tag="PLF66_14885"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HMZ65380.1"
                     /translation="MRIQGFAALNKKEELSEFQYDSKPIKDYDCIVKVHTCGICHSDI
                     HMMDNDWNMSSYPLVPGHEVVGVVEEVGKLVNHLKAGDRVGVGWQSGACLECEDCLRG
                     NENLCRRAESTIVGRHGGFASHIQLDSRFCFKIPDSIASEYASPLLCAGITVFSALRY
                     AGMTSGQNIGVIGIGGLGHLAVQFASKLGNRVTVFTTSPDKEEFALANGATEVVVGSP
                     KKVKNKLNIILNTTHNDLDWPRFLSFLDSDGTLSFVGVPPSTLSNVHVGMLLDRRLRI
                     MGSPIGGRAMIHDMLRISAEQKIRPVIERFTASNANAAITKVRENKIRYRAVLDFI"
     gene            complement(9047..14773)
                     /locus_tag="PLF66_14890"
     CDS             complement(9047..14773)
                     /locus_tag="PLF66_14890"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HMZ65381.1"
                     /translation="MQETLDYNRIETIFEGNKYSIFRAEGIDNRNVILKIFNSEHPTQ
                     IELNQIENEYKILEKLKKIEGVTQIITRGRFKGKIAFVFKNTKGESLEKLIHKKIPLY
                     TFFKIAKLIVAAIDRIHVQKIIHNDIKPSNIIYDSDTSNLEILNFGSSMEANGDYTLV
                     DISQRLDRDLSYISPEQTGRVNHAIDMKSDYYSLGATLFHLITGRPPFLVKDSIDLIY
                     SHIAKEPISPTKIDPNIPEGLSKIILKLLSKNPEERYHSSYGLLYDLESISSGQIIPE
                     DFQVGQKDFLSDFHIPKKLYGRENEVKQLTEVYYSMYNSGKPELALIQGGAGIGKSAL
                     VKGINEIVSDSRGFFVIGEYDQFNQSHAYSGIIKALSHLMQVLLTETEDEINNWRNRI
                     YSQMGTQIFSIVNIIPELELILGSEPPEEIQANEGLSINMQDIFLKFIRIFGTLHHPL
                     VLFLDDVQWADSASITLIQRLIQNQSLGYILIILGFRTSQRLINQSLSSMLDKLKKDK
                     IYPCTIRLKELNLKAITQFLSDSLFSTTDSVKDLADIVLGKTAGNPFFMNEFLKDLSR
                     ENLITIDRLQGKWVWDIEKIKKAKVSENIIELLVNQIKKLPILYQSILKTAACIGARF
                     DLWLLSISLDIPFQDLLNYLKRLTEQDFIYLSEPSIQKLKEYSNNIQSTNQAQEIIYC
                     FQHDRVFEAAYSLLDKEQRSTLQLSIGRFMLEGNFSSDTIQDIANHMNAGSEKIENPN
                     EIKRLIELNLEASQKAKNSTAHDVSLEYLKEAQKLLFRFEDPWSEIFNITLEVYKELS
                     ELYYLNVLFEEMEETTSIFLKYANNQSELIHGYRVLVNYYTTISNFAKAIEIAKESLN
                     RIDFYFPFQDFSNEREKCLNTVQNFLQNHSPEEILKLPKMESEKSRLVISMLSTLMPA
                     AYMYNIDLWTFLVLKSASIIFQEGICEEVYSLSGLGILLGIMHDDYKTGHQIALIALK
                     IAQGYKNQSEITKAANVLANYTLPFQKHLKEAKEINNICMEVGKNSAELQHLSYSLVF
                     DILNLFYSGVSLNKILNEVIPKNMVLCQHSKSLVGKDSINAIRLIILMMTTSSDDKME
                     QEVIREYAGRYLLQWKKNQNYSTVYIFKVLRAYFYYLNENYNDAYTQINRAKQYEQYA
                     TGMFTQAESVFIESLILCSIASNAKKEERNGYLEKIRKNQIILKKWSKSCPDNFFHKY
                     LLIEGEICRIKGHYFRASKYYDEAIEEAHTNEFIHIEALANEICARLYIKINKSRIMQ
                     SYINEAYYLYDRWGAVAKVKLLEEKFPEVFFKANRNTSEKKEVDQLINSIDLDAVIKA
                     SQALTEEIVLEKLLEKVMRVLFENAGAEKGLFIRYVEEEFKILAIGNSNQNDIEVVSN
                     QSEKSELSSEIAPKALIHYVARLKKYVVISDARKEDLFINDPYLRKKNPKSILCYPVL
                     RHERLIGLIYLENNYTINAFTKERLEVLAILSSQLAISIENSELYSNLEAKVTERTAN
                     LNQALHEVQLLKEKQDADYFLTSLLIHPLGKNKIQNDLIRIEFFIEQKKKFLFKNKQI
                     EIGGDLCTTDRIFLNGRSYIVVLNADAMGKSIQGAGGILVLGSVFKSIIQRTHSSTLN
                     ETLHPEKWIKNSFLEMQRVFESFDGYMLISMVLGLLEEETGIFYFINVEHPKLVLYRQ
                     KKAKFIKNVGRRFRKMGIEGIELPIQISLFKFLPEDVLILGSDGRDDIILGQDEYGLD
                     ILNFDENLFLEFVEEGRGNLKSIYEAIRKKGEVNDDLSLIRVSYKELNASVHSIENPP
                     LQDKKIELSKEIESLRKNRQLGETISLYEQIFYLDPMDTDLLIEFSNLLFQVKNYKKA
                     IEYLEIIKLRYPNKINNLQRLIISNIRLRNFQKADSLLAELLSLDSRNRKARFLENIL
                     SDRVIKSV"
     gene            complement(14781..15518)
                     /locus_tag="PLF66_14895"
     CDS             complement(14781..15518)
                     /locus_tag="PLF66_14895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017852230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HMZ65382.1"
                     /translation="MITTYLGIFLAFLSVIIAIILEGAHIGAFFKLSAIVLIVGGTLG
                     ATVASFSVIQIKAMLAILNDIITQQKRLDFSEIFIKLLEKTRRDGLLSLEDEIESIDN
                     DLAQKGLRLIVDGTDPSTVEDILFEWSEQKQEVEMNSAKILETAGGFCPTIGIIGTVM
                     GLVHVLENLGAGTTALGQGIATAFIATFYGIGFANLVFLPIANKVKAYSRTEHEVRQA
                     VIRGLLSIQAGDNKRIMLERMSPFLES"
     gene            complement(15518..17722)
                     /locus_tag="PLF66_14900"
     CDS             complement(15518..17722)
                     /locus_tag="PLF66_14900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65383.1"
                     /translation="MSKASNRFFVNSFGLRILPNILLCLSIGFLGSLVFKNAYQEIKL
                     LEKEDVFFQAAKLVLGASFLLGFILKRILHGMKAIYMEYSTLVLLISEMISLNGFSSG
                     ILQVDYIFYTDIFIFFASLNAFVLGLLISRMKNFRLWAFLSGGSVYIIYSLFNPEILI
                     KPETYVMPLVFVMTLELTLFSIIPKAHNAIHKESRKTHPINNVFFYTSLSLFVCHSIL
                     YYVNGKSIDSFVLGAGLGLLIFNIIFHYGLLNSMIGWKFLVGRVVVLVNLILTLHLSR
                     FELASGFLLFTDMACVAIYRPSRYKTIHVILAILVGFILYGITYFLNLYYLRYEFRYS
                     LIFAFLLLIWGPNLILNSIRLRSKIVTFLFSFLISAYFFSPIPYDYKMNFVKNESLKP
                     IPYLLSSIGLNEENFVYFNVNLPFKNQDTLPKKNTFRNKIVVLGLTDDLETTLTYLYH
                     LNKNGYPFVIFQDKKARQLTNKLIDFDSIEYPLFRIYFPRNTIPILQLEKENAAKSES
                     WEVQHIETRIRNLETNEEIADILINLQNYSAGSLRDTTILYRNKFYNSYKAYSEYYYK
                     AGDYDRAVQMAGLAFRFDVKDDSLLETAYNSILRITTEKEQIPVMRILLTKNRYKEQI
                     IKRLYPMLLTMKKKDEALEKISELIDYYSKDTEIDHSEDLKTLKIEKAKIYLQFSNIR
                     EAEEIIMTESRKGNGSIVWQKLMTDLITLKENLYKPYQHSGTNVQNTPRSIE"
     gene            complement(17793..19523)
                     /locus_tag="PLF66_14905"
     CDS             complement(17793..19523)
                     /locus_tag="PLF66_14905"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HMZ65384.1"
                     /translation="MKILILLIFLFGCGTKKANQNLLLSLITENPQVPKAFFSYPGRA
                     SDEIKKKEVIEAILDLINNSQFSLKIYAYSLNNPEIISALKEADKRGVKIEIVGDNDQ
                     KYDLLKENGLSVRVWKQSGLHHIKVILSDNKTFFTGTGNFSRYGLTKDWNGYIQFEIE
                     KINRARMNEFLSETLSERLFKTNGISFINSPEDGYLSQDLLLNSIDSAKSNIEFLIFD
                     QYDPVMSHSIRQASTRGIGITGVYNSPVDPEGLYLNDSFYGLGSNIYKDGNEDIIETE
                     SFSEGGLLHHKTMLIDRHTLLSGSYNYSTNARDSNREILFLTNNPILVTEFQREVDRV
                     KEASYLLPKKNYYHYPRIQRLLSKSINADILCLPESVNSPIIELGSGIWKTYLYYPKV
                     INKNCFSITDYANISSGLTHGSRSDFFSLTGLWDSFKVYDRSSDLVFLYESEYLDPIF
                     YSRKRFLSKNPKYLSFTNSRVYFEMREDIDFAGREMKLWIPGKEMRRGRLEQQPGTQT
                     AFFAPISTNSSERFFAAVWIEMPEEIYFFCYQDRNRTNRDSFEFILGKIEAAENLGGQ
                     FETACLSNSQ"
     gene            complement(19520..20833)
                     /locus_tag="PLF66_14910"
     CDS             complement(19520..20833)
                     /locus_tag="PLF66_14910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65385.1"
                     /translation="MKWMFVFMISLPIWAEYTIDVISAYKKTTEEKVEQNKTTVIENK
                     YSDNIIQLNTNMDGKRNLLEYKIQLSINKRSKDNDFKDILYLGDNCYLLLPIKKFQLV
                     LGRSLFPESLYLKKQWKDGMEGFGLKAQISDQFFLQVYLVDFYRGFPLFEKYFLYKNF
                     SEVNQKGERFRHGLTFVYRKETLFSKIQFTYLNLGNWGDGTKDDLKAQPKGDSDFIYK
                     TTFHISERLKYFTIGFELQIARGIDKVQYNPNRKEKSIPISGELICFYFESYWKNLKT
                     NFEVFLPDSDKRNKQGEVLESGFVGEGTHPTNALLLNQELNFYPSGWITPLGLQKVNS
                     YREGRMNSLWIHIGSSLRLDDLHFGLELDHLTPRLSSGASNGGISFQRGDYSNNYLAE
                     VTAFLLYDKRLTDGYFIKVNTSKLTTSKEIGLEGTSAYIQGGILF"
     gene            complement(20830..23826)
                     /locus_tag="PLF66_14915"
     CDS             complement(20830..23826)
                     /locus_tag="PLF66_14915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65386.1"
                     /translation="MKKYLFLFLILLVFACRSKKARTIWDFPTKPNTDSVYFTYGTKA
                     GSNTDVNPNQLPSWGPSLKITDQSICLESQDDFFKSKEYGPSLGLCLEPREDNKDLLT
                     TAIATLNELDESKIRPDGYLIQEEQKIHLYYQTITEIVNEISSSGSRTLFLGDSRLHN
                     ALSKRLDFSIENYKSVSGDREHRIETSFSLPYFSIFVLDKTVKIAFPPLESTTPRSQY
                     HYLVLDVTNLVDLVSLIESNFTRIQATNDTWRASCSREKLEITEVMGYASGVTKRFIE
                     WSSNSENPICLDTLQLSISGKKVTIDSNSTFLFPKAVKLFVEESSPLQGIVLNADWRE
                     IKGSAAIELISYDLKDNWNLPENVNFNWAEEEFSIKKRGNSCEWANSVLHNSKLCGDP
                     GIEFDENTVIPGTNVGVNHCKPEDFILTELNATGLKPENRMDRTGKFLEVKYLGGVSC
                     KLTGLFLNIGSIKVPLTMQALSLEPNSIWLVTDGRHVTGVKNLIRRNLDFLQWQHSIS
                     LQSSSSTKLLWKGLKAEEYFLNESSNGNLFSMVVDGESFYHHPVQKPNLLQAVYRQFH
                     SMSPGEANERVLQDAEGHISEVLWAGGYKNSSSILDEKFIELKTKGDGTLEVEVSLGN
                     KNYRYFIPISRNDAFTVLAKSNLQCFPDAKELVSDKLILSDEVTTIRLKSDGKIINEV
                     KYFPSRGNGFNNTSQKIRASYAYTGLASIWKTSSQPLRVSIASDCIGQTFATPNAPNE
                     FEPFLSEENFDYQGSGKFYLAASMWDINLPYSVKLYSKFPEEEAIIGLEKLSSNLLKQ
                     IPNLLNSIGLIYQKLENYSDLKIYNRDGIFIEGVMVNPSNPQNEWVLFCNRSKSIKDL
                     TEYEIEDEDSFDQIDTYFKRKKFNLPSGLNSSLFSGNTSVLQPGQCGYLVDPESSLLN
                     LKPVGVSPTLIYTVRTTSTIGNGISSGEYLDLYKVQGGNRFHIHSFGNRYSHSPFTIP
                     VITDEIILLQSNKRGEDRYDYEVVKW"
     gene            complement(23919..24548)
                     /locus_tag="PLF66_14920"
     CDS             complement(23919..24548)
                     /locus_tag="PLF66_14920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004752579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65387.1"
                     /translation="MQNSILKNKASITGLLLLFSSAILFSCSSTKEEVKDTKDLKTFY
                     PAGPKEKILDENGNEVEITSKDPELFQKDSKNPDELFRVVISSESYKVRQIRKTDLIK
                     RKPDPGGDSIIMEELIKYNKKDFVDDGIISVKLNTKTGKMETINTDRSTRIQQLLKII
                     SGDATRWILEHKNTEEPTVTKYLITYNVVLLNKATRDQIKEELKKEVRK"
     gene            complement(24875..26344)
                     /locus_tag="PLF66_14925"
     CDS             complement(24875..26344)
                     /locus_tag="PLF66_14925"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HMZ65388.1"
                     /translation="MLIFNSGLSFSKYAFFIYILGFAAVLANRFISVHIEIEDLNKNL
                     ESKVEERTKELNETLQEVKLLKEHQDGDYFLTTLIIQPLTSNVSGDEKVTFKTLIRQK
                     KKFEFKNRTHHIGGDICISEIIYLKGKKYIAFVNGDAMGKSIQGAGGALALGVVFKSI
                     LSRAKIFDKEESVFPERWLKLAFQELQSIFETFDGSMMVSVVMGLVDNDRGFLYYINA
                     EHPFCVLYRDGKASFIEKELSLHKIGMVLSEHSGEFKISTFKLENSDVIFMGSDGKDD
                     IRIGIDDKGGRIINEDESLFLATVEQADGNLEKIFELTEKKGEITDDFSLIRIEYNDN
                     SQIETEVDNSQIKLALEYYNKEEFRKAYEILLQIEPSNTKSYLRLMSNTTYRLGDYKQ
                     AAKLYTTFIDLYPEESNFLYAAAKSYRMIGDLEVAADYAERLKLREPKNVNNLIFLAE
                     VYTELKVTSRAYKLYILISRLDPENQKLGELKQKLNPVG"
     gene            complement(26284..27411)
                     /locus_tag="PLF66_14930"
     CDS             complement(26284..27411)
                     /locus_tag="PLF66_14930"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HMZ65389.1"
                     /translation="MKYLLLIFFLPFSVFPLDRIDLTKSCSNNICNPQAWYIINSYDS
                     SFLQLEEPDEKWKKVEAFPIWLNKFYQEEGSLATYTLLTYFDIPEDILNYPKHIGIRF
                     GEIGEVFEIYFNGTLIAKEGEVKDGKVSYHRTVRGVVYQVPRELMRASHNQLLVKLSG
                     HPKFDHTGFYLTRYYDFGFYDNIKYDEQDRVSLALMTVYMIVGLYHLFLYYRRRVELY
                     NFYYGAFSFIVGVYIYTRSSAIFENAWDTTIIQRVELAVLYPGFSFLSQSLDVLFFGK
                     SRKATRFYSYFSYIIGGLTIICPEMYQAEYILRLWQISILTIGIPLIGRIFYHAIKSS
                     VQNAKRLFVGLVIFIMAAIYDICSYLIRGFLSLNMHFLFIF"
     gene            27746..28249
                     /locus_tag="PLF66_14935"
     CDS             27746..28249
                     /locus_tag="PLF66_14935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65390.1"
                     /translation="MYQHNIKENLNTKFKIVMIFILLFNLSCATLLPTEKRIYSETVE
                     YKKSQDSWKKAVSSWVSTRFGERGLSTKKEEKDILSFVLGMGYDPKKTLPTVGSCHFI
                     LNVEYANNALNLHFENIFFVNGYGRVGTTARMHDGESGPNNEQELNSIIEKCIKVVAT
                     DLKDSIK"
     gene            complement(28626..>29139)
                     /locus_tag="PLF66_14940"
     CDS             complement(28626..>29139)
                     /locus_tag="PLF66_14940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65391.1"
                     /translation="LFQFNQWNDPVVVTHNLLPIFILFFLFILLFFFNSLFRFYGRNF
                     FILTLNFLSFLFIGIDIEPHYFEFWILPASLFTIYFSLFWNYIYEKSRDYFLLTPIAS
                     ILGIFFFVSASIVSIHNYRYELIFHSRNFKTEGVYEKIGETPIEYFKSSANYKYPNNP
                     YQEVDSFNLK"
BASE COUNT         9873 a         5122 c         4441 g         9703 t
ORIGIN      
        1 actggctcaa tatcatttaa gaactcaaaa tcctgagcaa tttccttttt atcaatcttt
       61 ttgataatct catcggcttt attgaaacct acagtttttg ataagagctc tctagcagtt
      121 tcaattcccc ccttctgtcc gagttgcaat tcagctacag tgtctttaaa ttcgtttaaa
      181 atggcttctc tttgaatttt tgataccatt cggatctggg acatctcata gacaacttgt
      241 tcaatcattt tatcatctaa gtgagaaaga acttttgcgg cttcttcttt tccgagagaa
      301 agaagtagta gagctgcttt tttagcacca ctcatagggg tatgcatttt attagagtcc
      361 atggcctgcg tgacataata aaaatgcttt agaaattaag tcaagaaaaa actttgtttg
      421 actgaaaagg caaatctgga cgaatcttac tatatcgaca catgtataat tccggatatt
      481 atatttccat aggcgctggc aagaatcaat tgcctctcat acataaagcc aagcaactag
      541 gacttaaagt catcgcagtc gatcaaaaca aagatgcagt cggttttaaa tactcagaga
      601 taaaaattct acactccatc ctggacttcc gtaaaattta tgctacagtc ttaaaactat
      661 ttttagaaga accaattata ggaataggag tgagatccta cggcaaagca gttttaactg
      721 cctcctattt agccgagaaa ttcaatctaa tcaatacaag cttagacatt ctaagaccct
      781 tctataacaa gaaaacaatg aaggcatttt taggacaaaa tggaattcct gttccaacct
      841 cttatatttg gagataccaa aaggacttct atgatttagt ttctaaggtc tcataccctt
      901 gtattttaaa acctgtagac aatattgcga aattgggaat agaaatattt gataacaaag
      961 aagaacttct aaatcgttta acacaactca aagctagaaa tgaaaaattc ttattagagg
     1021 aatttatcaa cggaaaagaa ataaccgttc ttggctttgt tcaaaaccaa aaatttacgt
     1081 tagtctctat atctgataaa atcaccactg actttccacc ctatttagaa ttatcacacc
     1141 aactcccaag taagcaagaa caacatctag gagaggtgca tctattatgc cagagtattg
     1201 taaatcttac aaatctagac aactctcctt ttgttgcaga atttaaaatc tccgacaatg
     1261 ggaaaattta tctaatagaa gtaatgcctg aaattggagg cgagtattta gcagatttat
     1321 taatcccaga attttaccaa tatgattatt tcaaaaatta tattaaaatg acgttaggtg
     1381 aaagaattga atcagttgat agccaaaaaa taaaaaagaa aaactccatt ggacaaatct
     1441 gttttatcgc tccacctgaa ggatcatcca agttcattca gtcaaacaat gtttcaataa
     1501 agggagatcg catttttcta aatgaaaaat taaaagatta caactcgata ttaaatacta
     1561 aagaaggaaa ctccgcacga gtaggagtaa taggtgttat ctcttcatcc aaaacaccag
     1621 aagaattaga taatgaaatt aggaaaaact tcaatgcaaa atttgaataa aataaatccc
     1681 actatttggg ataatcatta tacaaaagaa aagtccaagc ttgtattccc agatgaaaat
     1741 cttgttcgaa ttttatctag aattccagtt ggaacttctg ccttggacca tggggctggc
     1801 tcaggaagac actctgtatt gttaaaatct tatggatacc aggttacagc ttgtgactac
     1861 tccgcaaaag caattgaagg aatccaaaag atgcttccga ctataacgac taacgttata
     1921 acatcaacca ctctacccta tgaagataaa tccttcgact tagttgtaag ttggggagtc
     1981 ttacattaca atagttttga tgaagcaaat actataatct ccgaaatcaa acgaattcta
     2041 aaaccaaatg gctatatagt agggacagtc agatctaatt tggatactca tctaaaaact
     2101 aaggatggag tcattggaaa taaagatctt tctggaggca aagtaacatt atactcacta
     2161 gatgaattaa aagatatgtt aaaaattttt aaagatacag aatttggata tatggaaaga
     2221 acccctatgg gaaaattaga agagaggatc tgtcattgga tatttcaggc gaagaactaa
     2281 aaaagaactg ccctctagat aaagagtgcg attggtgttt tctttataga tccacttata
     2341 attattacga tctccctatt tataaatgta atacatgtgg gatacaaacg attcatccaa
     2401 aagataaata cgatttaaaa gcaatgtata actcaaatta ttattcaggg aatgcaagtt
     2461 actactacca tgatgaaaga aaaaatgaag tttacgaaag ttttgtttgg gatgcaagaa
     2521 ttcgtaacat taaaaaattt attcccaaag gtaatttttt agatataggc tcttcattcg
     2581 ggggattctt aaatagagcg aagatggctg gtttcgaaac ctttggtgta gagatttcag
     2641 aatattcagc tcagtatgca aatgaaagag gaattagaac ctataatgga tccttagtag
     2701 aggcagattt tccagccgaa ttctttgatg taataacgtt agtcgaagta attgaacatt
     2761 tagaaaaacc agatatagtt tttcctaaac tattcaagat tttaaaaccg ggtggactcc
     2821 tcttaattca aactgcaaat tttgatggaa tgcaggcaat ccaagaaggt cctaaatatc
     2881 attactatct ccccggtcac ctttattact actcccttag taatttaaaa aaaattttga
     2941 ccaaaaatgg atttaaagat ttcactgtct atcatggagt agactttccg ttgtatgcaa
     3001 aactttttaa atcaatggga agttttagaa aatggacaga ttattttaaa tggtttcgaa
     3061 tttcttatta tcatctaaaa agcaaatttt ttaagggaag cacttctagc atggttttat
     3121 atgctttcaa atgaggttta attatggcac agacaggatt agaaaaaatt aaaatttcag
     3181 ataagttcta cttgataccc gaaaagaaag gttttaaacg ttcttatatt atcttcctaa
     3241 gaatacttct tgtttcactt catcgatttt tagcagatga ttgcttaact cgtgcatcgg
     3301 ctatcgcata cacaaacatt gtatccctag ttcctgtatt aactgtggca cttgcacttc
     3361 ttacaattag ttcaggtttt accgaaaaac aagatgaact ttttgatagt atcaatactt
     3421 tcttacagaa gaatgagatt cgtatcgata ttactcccta tttggagaca ttaaaggata
     3481 taattaattc agctacacag attggggcaa ttggatttat cgttttgatc ttttctgcaa
     3541 cagcaatctt aagaaccttc gaagctgcat ttaatcagat ttggcgtata acgactccaa
     3601 gaccatttgc tgataaaatc attttttatt tttttatatt atctatcggt ccacttttag
     3661 gtgcaatctt cacaagtttc gctgctcgtt ttgctgatgc aacaagagct tcacatttgt
     3721 attcaatcac tcgttcctct gacaactttc tatggatggc gggagagaat ggaactatcg
     3781 ttaaaatcca agaaaacggg aagaggctag ccaaactcaa agattttaag atagattatg
     3841 aaaatatgta ttgtatgaat ctagataaag atgcagaagc aaactgtgat attcctaaac
     3901 taaataaaga aagttttgta aaaattagaa atcgaaaaaa tcagctagtt actatctctg
     3961 aaaatggagt gatcttacag tcttttgatt taggtcaaac ttggtctatt gcagaaattg
     4021 taaatgctaa agttttagat ttcactattg cagatgaaaa caatatgttt atagttttaa
     4081 cggatggtga aattataaat tatcacaata aagataaatt tgataaagta tttgtaaatt
     4141 actcaaactc agaagataaa gtgatcgcca ctaagattcg atttccagat ccagaacatg
     4201 gttatttttt agatgcaaat ggttattttt ggaaaacaag tgacggagga aaaacattcc
     4261 aaagcataaa agtcacaaaa ggaactctaa atttaactga ccttgcaatc ttagataatg
     4321 aaaattatgc agttgtagga gaaagaggag caatttatcg aacccatgat gcgggaaaaa
     4381 cctggataga catttctcat aaaagagttt cgtataccaa aatttggtat atgcaaaatg
     4441 aaggaaagga tgaactaatc gttttaaatg atttgggatc cattttgtat tctaatgatt
     4501 taggaaataa atggcagcta acgtatactc caacaaatgg taagttacat gcaatgatac
     4561 caatcaatcc aaaatttgga tttactgcca tgaatccaga ggatgaaagt gttcccacag
     4621 aagaggaaat tgcaaaacaa aaagaaaaac aaactgtagg cgatattttg gctgtaggag
     4681 aatttgggaa aatttccatt ggggatgtaa aagatggcac tcttgaatgg cacacaattg
     4741 caggaggaga caatattttt tccttatact cctttattaa aacggtaatt cctctatccg
     4801 ctatttggat cttttttatg atgctttata cactgattcc aaatacaaaa gttccattaa
     4861 aagccagtgc gattggtgct gctcttactg gttttatttt attgtctttc ttttgggctt
     4921 ttggaattta tttgagatca tttgcaactt ccactatggt aatatataga gcgttagctg
     4981 cagtcccaat ttttctttta gtcgtctact gtttttccat tatcgttttg tttggagctg
     5041 aaatcacagc tactctacat tataaagaca gatacaacag taacagacat ccctttgatg
     5101 atggcgaaca ggatataaaa tacacctatt ataatagtat taagtattta gtattgctct
     5161 atgaaaatca aagtaaaaac aaagcttttc taactgtaaa ttcactcaaa acgagtctaa
     5221 atctatcaac acttgatttt gatgctttaa cagaaaaatt agtatccgat aagtttattg
     5281 taaaggtaga aggaggtcag attgcacctt gttccatgcc atctgattta acgttatttg
     5341 atttatatga caaaatctca catgaatcct atgatcttcc tagctttgca gatcaaaaag
     5401 gatttgtaat taaaataagc gagcaattaa aagaagtgaa taatttttca aagcaaacac
     5461 tatctaagat aaaattttca gatttattgg tgtaaggtaa tttaaatgat taaatatgat
     5521 tatgactata ttgttgtagg ttctggtttt ggaggaagcg tttctgccat gagactttct
     5581 caaaaaggtt ataaagtggc tgtaattgaa tctggtaaaa gatggaaatc agatgatttt
     5641 cccaaaacta gtttttctct ttctaaatat gtatggtttc ctaaaatctt ttgctatggc
     5701 atacaacgta ttaacctctt aaatgatgtt atgatcctaa gtggagcagg agttggggga
     5761 ggaagtcttg tatatgcaaa tacactctac gtcccacctc aaccattctt tgaaacaccg
     5821 attgtgcaaa aaatgggagc agaaaaagga ctacttccat tttacgatat cgcaaaacga
     5881 atgttaggtg taattgagaa tccaaaactc tgggaagtag acgattacat gaaacaaaca
     5941 gccgatgaaa tgggagtcgg acataccttc aagagaactc cagttggaat caactttcat
     6001 gaaaaagaca aagtagatcc atattttaat ggagaaggac ctgaaagaaa tagctgcaat
     6061 ttctgcggtg gttgtatggt aggatgtaga ttcaatgcaa aaaatacttt agataaaaac
     6121 tatttgtatt ttgcagagaa acttggagca accattattc ctgaaacaaa agtagataag
     6181 ctaattccac tttcaccaga tggtggcgat ggatatgaag tgcagactcg atctaccact
     6241 ggattctttg gcaaccctag aaaaacattt agagctagaa acgttgtatt atcagcagga
     6301 gttctaggaa cgttaggcat tcttcttaaa atgcagcaag aaaagatcat gactcgactt
     6361 tctccaatgc taggaaagac agttagaacc aatagcgaat ctttagtagg tgttacatct
     6421 ttaagaaaag gagtcgattt ttcaaaagga attgctatta catctagtat ttatcctgat
     6481 gagcatactc atattgaacc agttcgttac tcagctggtt ctgatgctat gggaacgatt
     6541 gctgctggaa cgttagttga tggcggaaaa ggtgttcctc gatttttgag attcctatgg
     6601 agttgtgtcc aacatcctat caatacgatt cggtttttgt ctccctttgg atttgccaaa
     6661 aagtcaatca ttctattagt tatgcaaact gtagataata gtataaggat tgtaagaaaa
     6721 agaagactca tctggccttt ccaaaagaca ataacttcaa cacaagaaac aggaacacat
     6781 attccaactt ttattccaat cgcaaatgaa tttgccagac gtttagcaaa acgaattgga
     6841 ggagtggcaa gaagttcttt aaacgaagtt atactggata taccaactac cgctcacatt
     6901 ttaagtggat gtatcattgg agatagtcca gaaacaggag tgatcgatct aaaaaatcag
     6961 gtttatggat ataagaattt actgatttgt gatggttcta tggtgcctgc caacctaggg
     7021 gtgaatccaa gtttgaccat aacagctttg tcagagcgag caatgtcttt tgttccactt
     7081 gcttctgaag tgaaagaaca tagatttttc gattatgaaa aaaattggaa aataaccgat
     7141 gctcttacag gaaaagaaat aaagagttca gagactcagc ccaaagatgc agagaaaaaa
     7201 gaaaactcta aatctatttc aaagcctaaa gtatcaaaca aagataccaa agctgcaaag
     7261 ccagctccag caaagaaaaa agcaactcaa gtcaaagcaa aaaagaaaat aaaaaaataa
     7321 tccagaatat acacgaagaa agccttagca ttcttcgtgt atattactaa aaacatttct
     7381 ttctcattaa taaaaaccta agaattcttc cttgcaactc tttcaccaat cagaatcggc
     7441 ttaaataatt ccttcaaaat tcaaattatt ccgatactag aaaaagatat acttagatcg
     7501 agagtgctat atgaatttta aaacctattt ttttgtatta ttacttttat ctctaccgtt
     7561 agccgcacag ggaaatatta aatgcataga tggggattgt gtcaatggaa atggaatcac
     7621 cgttgatgac gcaggtatcc gctatgaggg ctcatttcaa aatggacaaa aaaatggaaa
     7681 gggaactcaa attgaaccga atgggaatat ttataaagga gactggaaga ataatctacc
     7741 aaacggcaaa ggcacctaca cactcagatc gggggaaact cgtaaatata caggatcatt
     7801 caaagatgga tattacaacg gtgtaggaac tctacaatat gcaaatggag ataaatacat
     7861 tggcgaattt aaaaacgaca aacgcagcgg atacggaact tttatctcaa aagcagagag
     7921 aaagatttac atagggaaat ggaaaagcga taaacaaaac ggcaaaggta tatactatgc
     7981 atatacctct accgaaaaag ttaaatttaa taaaggctct ctagttctta gataaaatct
     8041 aaaactgctc tatagcgaat tttattttct cttacttttg taattgctgc attcgcattg
     8101 gatgcagtaa atctctcaat gacaggtcga attttttgtt cggcagaaat tcttaacata
     8161 tcatgaatca tagctcttcc acctattgga cttcccatga tcctgagtct tctatctaat
     8221 aacattccta catgaacgtt agacaaagtg cttggaggaa ccccaacaaa actcaaagtc
     8281 ccatcagaat ccagaaaact taaaaagcga ggccaatcta aatcattgtg agttgtattc
     8341 agaatgatat tgagtttatt tttaactttt ttaggagaac caacaacaac ttcagtagcc
     8401 ccatttgcaa gagcaaactc ttctttatct ggacttgtag taaagacagt cactctattt
     8461 cctaatttag atgcaaactg aacggctaaa tgacctaatc ccccaattcc aataactcca
     8521 atattttgtc ctgatgtcat gcctgcataa cgaagagcag aaaacactgt tattcctgca
     8581 caaagtaacg gagatgcata ctctgatgca atggagtcag gaattttaaa acaaaatcga
     8641 ctatctaatt ggatatgact tgcgaatcca ccatgtctgc caactatcgt gctttctgct
     8701 ctacgacaaa gattttcatt tccacgtaaa caatcttcac actctaagca agctcctgat
     8761 tgccaaccaa ccccaactct atctccagcc ttcaaatggt ttactagttt ccctacctct
     8821 tccactacac ctacaacttc atgccctggc actagtggat agctagacat attccagtca
     8881 ttatccatca tatgaatgtc gctgtgacaa attccacatg tatgaacttt tacaatacaa
     8941 tcataatcct ttatgggttt tgaatcatat tgaaattctg atagctcttc ttttttgttt
     9001 agagcggcaa atccttgtat tctcataact cctccatttt aaatatttat accgatttta
     9061 taacacgatc agatagaatg ttttcaagaa acctggcttt gcgatttcta gaatctagtg
     9121 acaaaagttc tgccaataga ctatccgctt tttgaaaatt tctaagacga atatttgata
     9181 taatcaaacg ttgtaaattg tttatcttat tcggatatct aagtttaatt atctctaaat
     9241 attcgattgc ttttttataa ttcttaactt gaaacaataa atttgaaaac tcaattaata
     9301 gatctgtatc cattggatcc aaatagaata tctgctcata caaagaaatt gtttctccta
     9361 attgtctatt ttttcgtaga ctttctattt cctttgacaa ctctattttt ttgtcttgta
     9421 acggtggatt ttctattgaa tgaacagaag catttagttc tttataactc actcgaatta
     9481 gagacaaatc atcattaacc tctcccttct ttcgaattgc ttcatatata gacttcaaat
     9541 tccctcttcc ctcttccaca aactctaaga ataaattctc atcaaaattt aatatatcga
     9601 gaccatactc atcctgacct aaaataatat catctctacc atctgaccca agaatcaaaa
     9661 catcctccgg aagaaattta aaaagtgata tttgtatcgg aagttcaatt ccttctatac
     9721 ccatttttct aaatcgtcta ccaacattct taataaattt agctttcttc tgtctatata
     9781 aaaccaattt aggatgttca acatttataa aatagaagat tcctgtctcc tcctccaata
     9841 atccaagaac cattgatata agcatataac catcaaaact ctcaaagact cgttgcatct
     9901 ctaagaagct attcttaatc catttttcag gatgcaaagt ctcatttaaa gtcgaagaat
     9961 gtgttctttg aatgattgat ttaaatactg atccaagaac aaggattcca ccagcccctt
    10021 gaattgattt ccccattgca tctgcattta aaacaacgat atacgatcta ccatttaaaa
    10081 atattctgtc ggtagtgcat agatctccac caatctcaat ctgtttgttt ttgaataaaa
    10141 actttttctt ttgttcaata aagaattcaa ttcgaattaa atcattttgt attttattct
    10201 ttcctaatgg atgaattaaa agagatgtta aaaaataatc tgcatcttgc ttttctttta
    10261 ataactgaac ttcatgcaac gcttgattta aattggctgt tctctctgtt acctttgcct
    10321 ccaaattaga gtataattct gaattttcta tagaaattgc gagctgacta gaaagtattg
    10381 ccaaaacttc taatctctct tttgtaaatg cgttaatagt gtaattattt tctaagtata
    10441 ttaaacctat gagtctttca tgccttaaaa ctggataaca aagaatcgac tttggatttt
    10501 tttttcttag atatggatca ttgataaata aatcttcttt tctcgcatct gagataacaa
    10561 cgtatttttt tagccgagca acataatgta taagagcctt tggtgcaatc tcagaactta
    10621 gttctgactt ttcagattgg tttgatacta cctcaatatc attttgattt gaatttccga
    10681 ttgctaatat cttaaactct tcttccacat atcgtataaa cagacctttt tcagcacctg
    10741 cattttcaaa taaaacgcgc ataacttttt ctaataattt ctccaacaca atttcttctg
    10801 ttaaagcttg tgaggctttt attaccgcat ctaaatctat actgtttatc aattgatcta
    10861 cttctttttt ttcagaagta ttgcgatttg ctttaaaaaa tacctcagga aatttttctt
    10921 ctaataattt aactttagcg actgcgcccc agcgatcata taaataataa gcttcattga
    10981 tataactttg cattattcta gacttattta tttttatata caatcttgca catatttcat
    11041 ttgccaatgc ttcaatatgt ataaactcat tcgtgtgtgc ttcctcaatt gcctcatcat
    11101 aatatttact agcacgaaaa taatgacctt taattctaca aatttcacct tctattagaa
    11161 gatacttatg aaaaaaatta tcaggacaag atttactcca ttttttaagt atgatttgat
    11221 tttttctaat tttttctaag tatccatttc tttcttcttt tttagcgtta ctcgcaatag
    11281 agcatagaat caaagattca ataaatacag attcagcttg agtaaacatt cctgttgcat
    11341 attgctcata ctgctttgct ctattaattt gtgtatatgc atcattgtaa ttttcattta
    11401 aataataaaa gtaggctctc agaactttga aaatataaac tgtgctataa ttttgatttt
    11461 ttttccattg aagtaaatat ctacctgcat actcccgaat tacctcctgt tccattttat
    11521 catctgaact tgtggtcatc attagtataa ttaaacgtat cgcattaatt gaatcttttc
    11581 caacaagact tttggaatgc tgacacaaaa ccatattctt aggaatcact tcatttaata
    11641 ttttattcaa gctaactcca gaataaaata aattcaatat atcaaaaact aagctataac
    11701 ttaaatgttg taattcagcc gagtttttac ctacctccat acaaatattg ttaatttctt
    11761 ttgcttcttt taaatgtttc tggaaaggca aagtataatt agccaaaaca tttgcagctt
    11821 tagtaatttc agactgattt ttatatccct gagcaatctt taaagcgata agcgctatct
    11881 gatgacccgt tttataatcg tcatgcataa tccctaataa tattccaaga ccggatagag
    11941 aatagacttc ttcacaaatg ccttcctgga atattattga agctgattta agaacaagaa
    12001 aagtccataa atcgatatta tacatatatg cagcaggcat aagagtagaa agcattgaaa
    12061 tcaccaacct agatttttca gattccatct tgggtaattt taggatctcc tcgggagaat
    12121 gattctgtag aaaattttga actgtattca aacatttttc tctctcattc gaaaaatctt
    12181 gaaacgggaa ataaaaatct atacgattca aagattcttt agcaatctca atcgcttttg
    12241 caaaatttga aatagtagta taataattta ctagaacacg atagccatgt attaattcag
    12301 attgattatt tgcatatttt aaaaatatag aagtagtctc ttccatttcc tcaaatagaa
    12361 catttaaata atagagctca gataactctt tgtaaacttc tagtgtaata ttaaagattt
    12421 cagaccaagg atcctcaaaa cggaaaagta atttttgtgc ttcttttaaa tactctaaag
    12481 aaacatcatg agctgttgaa ttttttgctt tctgtgaagc ttctaggttg agttcaatta
    12541 atcttttaat ttcattcgga ttttcaattt tctcagaacc tgcattcata tgatttgcaa
    12601 tgtcctgaat agtatcgctc gaaaaattcc cttccaacat aaatcttcca atcgatagtt
    12661 gcaatgttga tctttgctct ttatctaata aactatatgc agcttcaaaa acacgatcgt
    12721 gctgaaagca gtaaataatt tcttgtgctt gatttgtgga ctgaatatta tttgaatact
    12781 cttttaattt ttgaattgag ggttctgata aatatataaa atcttgttct gttaaccgtt
    12841 ttaaataatt cagtaaatct tgaaaaggta tatcgagaga aatactaagt agccaaagat
    12901 caaacctagc tccaatacaa gcagctgttt ttaaaatact ctgatatagg attggtaatt
    12961 ttttaatttg atttaccaaa agttctataa tattttctga gacttttgct ttttttattt
    13021 tctcaatgtc ccaaacccat tttccttgca acctatcaat ggtaatcaaa ttctctctag
    13081 ataaatcctt taagaattca ttcataaaaa aaggatttcc agcagtctta cctaagacaa
    13141 tatcagccaa gtcttttact gaatctgttg tagaaaaaag agaatcagat aaaaactgtg
    13201 ttattgcttt taaatttaat tcttttaatc gaatcgtgca cggatagatt ttgtcttttt
    13261 ttaacttatc aagcatagag gataaacttt gattgattaa ccgctgactt gttctaaagc
    13321 cgaggataat taatatatac cccaaggatt gattttgaat taatctttga attaatgtta
    13381 tggaagcaga atctgcccat tgaacatcat ccaaaaataa taccaatgga tgatgaagag
    13441 tcccaaagat tctaataaac tttagaaata tgtcttgcat gtttatagaa agaccttcgt
    13501 tagcttgaat ctcttcaggt ggctctgatc ctaaaatgag ttctagttca ggaattatat
    13561 tcacaattga aaagatttgt gttcccattt gagaataaat tcgattcctc caattattaa
    13621 tctcatcctc agtttcagtt aataaaactt gcatcaaatg agatagggct tttataattc
    13681 ctgaataagc atgactttga ttaaattggt catattcccc gatgacaaaa aatcccctac
    13741 tatctgagac tatctcattt atccctttaa cgagagcaga ttttccaatc cctgctcctc
    13801 cctgaattaa ggctaactct ggtttgccgc tattatacat tgagtagtag acttctgtta
    13861 gctgttttac ttcattttct cttccatata attttttagg tatatggaaa tcggataaaa
    13921 aatctttttg tccaacttga aagtcttccg gtataatttg ccctgaactt atcgattcca
    13981 aatcatataa aagtccataa gaactatgat atctctcttc tggattcttt gatagtaact
    14041 ttaagattat ttttgaaaga ccttctggta tatttggatc tattttagtt ggggatattg
    14101 gctcttttgc aatatgacta taaatcaaat caatggagtc tttcactaag aagggaggtc
    14161 tacctgttat taaatgaaag agtgtagcgc ctaacgaata ataatctgat ttcatatcaa
    14221 tcgcatgatt cactcttcca gtttgttcag gcgatatata agatagatct ctgtccaatc
    14281 tttgactgat gtcgacgagt gtataatcgc cgttagcctc catactagac ccaaaattca
    14341 atatttctaa attacttgta tcagaatcat agattatatt ggaaggtttt atatcattgt
    14401 ggattatttt ttgaacatgg attcgatcaa ttgcagctac aatcagcttt gcaattttaa
    14461 aaaaagtata aagcggtatt tttttatgaa ttaacttctc taggctttcg cctttagtat
    14521 ttttaaaaac aaaggcaatt ttacctttaa aacgtcccct tgtaatgatt tgcgttacgc
    14581 cttctatctt ctttaatttc tcaagaatct tatactcatt ctctatttga tttaattcta
    14641 tttgcgttgg gtgctctgaa ttaaatatct ttaagattac gtttcgatta tcaatacctt
    14701 ccgctcgaaa tatactatat ttatttcctt caaatatagt ttcgattcta ttataatcta
    14761 acgtctcttg catgaaacga ctaagattct agaaaaggag acattctttc taacataatt
    14821 cttttgttat ccccagcttg aatagagaga agaccgcgaa tcacagcctg cctcacttca
    14881 tgttcagtcc tagaataagc cttcacttta tttgctatag gcagaaatac aaggttggca
    14941 aaaccaattc cataaaaggt tgctataaat gcagtcgcta ttccctgacc aagtgctgtt
    15001 gtgcctgcac ccagattttc caatacatgc acaagaccca taacagttcc aataattccg
    15061 atcgtaggac aaaaaccacc agctgtctct aaaatcttag cagaattcat ttccacttct
    15121 tgcttttgct ctgaccactc aaataaaata tcttcaactg tagatggatc tgttccatct
    15181 acaattaatc gcaaaccttt ctgagctaaa tcattatcaa ttgattcaat ttcatcttcc
    15241 aaagagagaa gcccatctct tctcgtcttt tctaataact ttataaaaat ttcagagaaa
    15301 tccaatcgct tctgctgagt tattatatca ttcaaaatcg caagcatagc cttgatttga
    15361 ataacggaaa aactagcaac agttgctcct aacgttcctc ccacaattaa aacgatagca
    15421 gataatttaa aaaatgctcc aatatgagca ccctctaaaa taattgcgat tatgacagat
    15481 aaaaatgcta aaaatattcc aagatatgtt gtaattatca ctcaatactc ctcggtgtgt
    15541 tctgaacgtt agtccccgaa tgctgatagg gtttatataa attttctttt agtgtgatta
    15601 ggtcagtcat aagtttttgc caaactatcg atccattgcc ttttctagac tcagtcataa
    15661 taatctcttc tgcttctctg atatttgaaa attgaagata gattttcgcc ttctctatct
    15721 tcagagtttt taagtcctca gaatgatcga tttcagtatc ttttgaatag taatcaatca
    15781 actcagaaat tttctctaaa gcttcatctt tctttttcat tgttaaaagc attggataca
    15841 atcgcttaat aatctgttct ttgtatctat tttttgtcag gagaatacgc ataacaggta
    15901 tttgctcctt ctctgtggta attctaagta ttgaattgta agcagtctct aaaagactat
    15961 cgtctttcac atcaaatcga aatgcgagtc ctgccatctg aacagctcga tcataatcac
    16021 ctgctttata ataatactct gaataagcct tgtaagaatt ataaaactta tttcgatata
    16081 aaatcgtagt atctctgaga gagcctgcag aataattttg aaggttaatt agaatatctg
    16141 caatttcttc attggtctct aaatttcgaa ttctagtttc aatgtgttga acttcccaac
    16201 tctcagactt tgccgcattt tccttttcta gttgtaaaat cggaatcgta tttctaggaa
    16261 aataaatacg aaaaagcgga tattcaatgg aatcaaaatc gattaatttg tttgttagtt
    16321 gtcttgcctt tttgtcttga aaaatgacaa atggatatcc gttcttgttt aaatgatata
    16381 gataagtcaa ggtcgtttct aagtcatcgg tcaaacctaa gacaactatt ttattacgaa
    16441 aagtattttt ctttggtaat gtatcctgat ttttaaatgg taagtttaca ttaaagtaaa
    16501 caaagttttc ttcatttaat ccaatcgaac tcagtaaata tggaatcggt tttaagcttt
    16561 cattttttac aaagttcatt ttataatcat agggaatagg tgaaaagaaa taagcggata
    16621 ttaaaaaact aaaaagaaaa gtaacaattt tacttcgcaa gcgaattgaa tttaaaatta
    16681 aattcggtcc ccaaattaat aaaagaaatg caaatataag agaatatcta aactcgtagc
    16741 gaagatagta tagatttaaa aaataagtga tcccatataa aataaatcca actagaatcg
    16801 caaggataac atgtatcgtt ttataacgac ttggacgata gatggcaaca catgccatat
    16861 ccgtaaaaag caaaaatcca ctcgccaact caaatcgaga aagatgcaag gtaagtatca
    16921 gattcaccaa tactacaact cttcctacta aaaacttcca gccaatcatt gaatttaaaa
    16981 gaccgtaatg aaaaataata ttaaagatca aaagtccgag cccagctccc aaaacaaaag
    17041 agtctataga ctttccattt acgtaataga gaatggagtg acaaacaaac aaagaaagtg
    17101 atgtgtaaaa aaatacatta ttaattggat gagtctttct gctttcttta tgaatagcat
    17161 tgtgtgcttt tggaataatt gaaaataacg ttagttccag tgtcatcaca aacactaaag
    17221 gcattacata cgtttccggc tttattaata tctcggggtt aaatagggaa taaatgatat
    17281 aaacactccc cccacttaaa aatgcccaaa gtctaaaatt cttcattcta gaaataagaa
    17341 gacccaaaac aaatgcattc agacttgcaa aaaaaataaa aatatctgta taaaaaatat
    17401 aatcaacttg gagaattcca gatgaaaatc catttagact aatcatttcc gaaatcaata
    17461 aaaccagtgt cgaatattcc atatagatgg ctttcattcc atgtaatatt cgtttgagga
    17521 taaaaccaag aagaaaggag gctccgagca ctaactttgc cgcttgaaag aaaacatcct
    17581 ccttttcaag aagcttgatc tcttggtaag catttttaaa aaccaatgag cctaaaaagc
    17641 ctatagataa acataaaagg atattgggaa gaattctaag cccgaaagaa ttcacaaaaa
    17701 atcggttgct ggctttactc atgggttctc aactactaaa cttccctaag attggttttt
    17761 tggaactaaa aaaaatttca ggatcaaatt tgctactgag aattggacaa acaagcggtt
    17821 tcaaattgcc cgccaagatt ctctgccgcc tcaattttac ccagaataaa ttcaaaagaa
    17881 tcccgattgg ttctatttct atcttgatag caaaaaaaat aaatttcctc aggcatttcg
    17941 atccatacag ctgcaaagaa tcgttctgac gaattggtag aaattggagc aaaaaaggcg
    18001 gtctgagttc caggctgttg ttccaatctc cctctccgca tctcttttcc cggaatccag
    18061 agtttcattt ctcgtcctgc aaaatcaata tcttccctca tctcaaaata aactctagaa
    18121 ttagtaaaag agagatactt tggattttta gaaagaaacc tttttctaga ataaaaaatt
    18181 ggatctaaat actcagactc atacaagaaa actaaatcac tagaacgatc atagaccttg
    18241 aaagaatccc aaagtccagt cagtgagaaa aaatcagatc tactcccatg agtgagaccg
    18301 gaagatatat ttgcataatc tgttatagaa aaacaatttt tatttatcac tttaggataa
    18361 tataagtatg tcttccagat accactgcct aattcaatga taggagaatt tacactttca
    18421 ggcaaacaaa gaatatccgc atttatactt ttagataata atctttgaat ccgaggatag
    18481 tgatagtaat ttttcttggg caataaataa gaggcttctt ttaccctatc tacctctctt
    18541 tgaaattcag ttactaaaat aggattgttt gttaaaaata gtatttctcg attcgaatcg
    18601 cgtgcgttag tagaataatt ataagaacct gacagaagtg tatgtcgatc tatcaacatt
    18661 gtcttatgat gcagtagccc tccttcagaa aatgattctg tctcaattat atcttcattt
    18721 ccatctttgt aaatattaga acccaatcca taaaaagaat catttagata taaaccttct
    18781 ggatcgacag gcgaattata aacaccagtg attccaattc ctcttgtaga tgcttgtctg
    18841 atcgaatgac tcataacagg atcatactga tcaaaaatta gaaattcaat attcgatttg
    18901 gcagagtcta tactgtttaa taataaatct tggcttaaat atccatcctc aggagaattt
    18961 ataaaggata taccatttgt tttgaataat ctttcgctta atgtctcact taaaaattca
    19021 ttcatccttg cacgatttat tttttctatt tcaaactgaa tgtatccatt ccaatctttt
    19081 gtgagaccat atctagaaaa atttcctgtg ccagtaaaga aggttttatt gtcagaaaga
    19141 attactttaa tatgatgtag accggattgt ttccaaactc ttacactcaa tccattttcc
    19201 tttaatagat catacttttg gtcattgtct cctacgattt caattttaac tcctctttta
    19261 tctgcttctt ttaaagcgga tataatttct ggattgttta aagagtaagc atatatcttt
    19321 aaactaaatt gggagttatt gattaaatct aaaatagcct ctatgacttc tttttttttt
    19381 atttcatcac ttgctctacc tggataagaa aaaaaagctt ttggaacttg ggggttttct
    19441 gttattaaac ttaaaagcag attttgattt gctttctttg ttccacagcc aaatagaaat
    19501 attaataaaa ttaatatctt caaaataata ttcctccctg tatataagcg gaagttcctt
    19561 ctaatccaat ttctttagaa gtcgtaagtt tagatgtatt tacttttata aagtatccat
    19621 ccgttaaacg cttatcgtat aatagaaatg ctgttacttc tgctaaataa ttattggaat
    19681 aatcacctct ttgaaaagag atacctccat tcgatgcacc agagcttaat ctaggagtga
    19741 gatgatctaa ctctaaacca aaatgtaaat catctaagcg taaactagaa ccaatatgaa
    19801 tccaaagtga attcatccta ccctcacgat aggaatttac tttctgaaga cctagaggag
    19861 taatccaacc ggatggatag aaattcaatt cttgatttag taataatgcg ttagtcggat
    19921 gcgttccttc tccaacaaat cctgattcta aaacttctcc ttgtttattt cttttatcag
    19981 agtctggtaa gaaaacttcg aaatttgttt ttaaattttt ccaatatgac tcaaaataga
    20041 aacaaatcaa ttcccctgat atcggtattg atttctcttt ccggtttgga ttgtattgaa
    20101 ctttgtctat accgcgagca atttgcaatt caaatccgat cgtaaagtat tttaaccttt
    20161 ccgatatatg aaaagtagtt ttatatataa aatcactatc tccttttggt tgtgctttta
    20221 gatcatcttt tgtcccatct ccccaatttc ctaaattcaa ataagtaaat tgaatttttg
    20281 aaaataaagt ctccttacgg taaacaaaag ttagtccatg cctaaatctt tctcccttct
    20341 gatttacctc tgagaaattt ttatataaaa aatacttttc aaaaagagga aaacctcgat
    20401 aaaaatcgac aaggtaaact tgtaaaaaaa attgatcgga gatttgggct tttaacccaa
    20461 atccctccat tccatctttc cattgttttt taagatataa actttcggga aaaaggcttc
    20521 ttcctaaaac gagttgaaac tttttaattg gaagaagcaa ataacaatta tccccaagat
    20581 atagaatatc tttaaaatca ttatcttttg atcttttatt tatactcaat tgtatcttat
    20641 actctaatag atttctctta ccatccatat ttgtatttag ctgaatgata ttatcagagt
    20701 atttgttttc tatcacagta gttttattct gttccacttt ttcttcggtt gtttttttat
    20761 acgctgagat tacatctatc gtatactcag cccaaatagg aagagaaatc ataaaaacaa
    20821 acatccactt caccacttta caacctcata gtcatagcga tcctctcctc ttttatttga
    20881 ttgtaaaagt ataatctcat cagttatgac tggaatagta aatggagaat gcgaatacct
    20941 atttccaaag gagtgaatat gaaaacgatt tcctccctgc actttataga ggtctaaata
    21001 ttcccccgat gaaataccgt ttccaattgt agacgttgtg cgaactgtat aaatcagagt
    21061 aggagaaaca ccaactggtt ttaaatttaa tagagaagat tccggatcta ctaaataccc
    21121 acactgacca ggctgaagaa cactcgtatt gcctgaaaac aaggagctat tcaaaccgga
    21181 aggtaggtta aattttttcc tcttaaagta agtatctatt tgatcgaaag aatcctcatc
    21241 ttctatttcg tattcagtta aatcttttat actcttactc ctgttacaaa acaagaccca
    21301 ttcattttga ggattactag gattcaccat aaccccttcg ataaaaatac catcccgatt
    21361 gtagattttt aaatcagaat aattttccaa tttttgatat ataagaccta tactatttaa
    21421 taaattagga atctgtttta atagatttga agatagtttc tctaatccaa ttatagcttc
    21481 ttcttctgga aacttactat atagttttac tgaatatgga aggtttatat cccacatgga
    21541 tgcggctaaa tagaattttc cgctcccttg ataatcaaaa ttttcctctg acagaaatgg
    21601 ttcaaattca ttcggagcat ttggtgttgc gaatgtttga ccaatacaat ctgaggcaat
    21661 actgactctt agtggctgac tagaagtttt ccaaatagaa gcaagtcctg tataggcata
    21721 acttgctctg attttctgac ttgtattatt aaatccatta ccacgggatg gaaaatattt
    21781 tacttcattt atgattttac cgtccgactt aagtctgatc gttgttactt catcacttaa
    21841 tattaattta tctgatacta attcttttgc atctggaaag cattgtagat tagattttgc
    21901 taaaacagta aaagcatcat ttctagaaat tggaatgaaa taacgatagt tcttattccc
    21961 taaagaaact tctacttcga gtgtgccatc cccttttgtt tttagctcaa tgaatttttc
    22021 atctaaaata gaactagaat ttttatatcc accagcccat agaacttcag agatatgccc
    22081 ctccgcatct tgtaatactc tttcatttgc ctcaccagga ctcatcgaat gaaattgtct
    22141 ataaactgct tgtaatagat ttggtttctg aactggatga tgataaaaac tttctccatc
    22201 caccaccatt gaaaataaat ttccattact tgattcattt agaaaatatt cctctgcctt
    22261 taaacctttc cataaaagct ttgtgctaga actagactgg aggctaattg agtgttgcca
    22321 ctgaaggaaa tctaaatttc ttctaattaa atttttaact ccggtaacat gtctcccatc
    22381 tgtaacaagc caaatggagt ttggttctaa actgagggct tgcatagtaa gtggaacctt
    22441 tattgaacca atatttaaaa acaagcctgt taacttacaa gatactccgc ctagatattt
    22501 gacttccaag aattttccag ttctatccat tcgattttcc ggtttgagtc cagttgcatt
    22561 taactcagtc aaaataaaat cttccggctt acaatgattt accccaacgt ttgttcctgg
    22621 aatgactgtg ttttcatcaa actcaatgcc tggatcacca caaagcttag aattatgcaa
    22681 aacagagttt gcccactcgc atgaatttcc cctcttttta atagaaaatt cttcttcagc
    22741 ccaattgaaa tttacatttt ctggcaaatt ccaattatcc tttagatcat aggatattaa
    22801 ttcgatcgct gcagagccct taatctctct ccaatccgca tttaaaacaa tcccttgcaa
    22861 tggagaactt tcttctacaa ataacttaac cgcctttgga aataaaaagg tagaattgga
    22921 atcaatcgta actttttttc ctgaaatcga tagttgcaat gtatctaagc aaataggatt
    22981 ttcagaatta cttgaccatt cgataaatcg tttggtgaca ccggaagcat atcccatcac
    23041 ctctgtaatt tctaattttt cacgactaca gcttgctctc catgtatcgt ttgtcgcttg
    23101 tattcttgta aaattcgatt caattaaaga tactaagtct actaaattcg ttacatctaa
    23161 aactaagtaa tgatactggg atcgaggagt cgtagattct aaagggggaa aagcaatctt
    23221 aaccgtcttg tctaaaacaa aaatagaaaa ataaggaagt gaaaaactag tttcaattct
    23281 atgttctcta tctccagaga cagatttgta attttctata ctaaaatcta accgtttaga
    23341 aagggcattg tgtagtctac tatcgcctaa aaataaagtt ctagaaccac tcgaactaat
    23401 ttcatttacg atttcagtta tcgtttgata ataaaggtga atcttttgct cttcttgaat
    23461 gagataacca tcgggtctga ttttagattc atctaattca tttaaggtag caattgcagt
    23521 tgtgagtaaa tctttattat cttctcttgg ctctaaacac aaaccaagac taggtccata
    23581 ttcctttgat ttgaaaaaat catcttggga ctcaagacag atggactggt ctgttatttt
    23641 taaggaaggt ccccaagaag gaagttggtt tggatttaca tctgtattac ttccagcttt
    23701 tgttccataa gtaaaataga ccgaatcagt gtttggttta gttgggaaat cccagatagt
    23761 cctcgctttt ttagaacgac aggcgaagac taatagaatt aaaaatagaa aaagatattt
    23821 tttcatttgt ttctcctttt cttaacaggg tcaaaaaaga gaaactatgt gcggaaaaaa
    23881 tttaattcat tacgcagttg cgcaattttt ttttattttt atttacgaac ctctttttta
    23941 agctcttcct tgatctggtc acgagtcgct ttattgagta ataccacgtt gtatgtgatt
    24001 aagtattttg ttactgttgg ttcttccgta tttttatgct ctaaaatcca gcgagtcgca
    24061 tcaccggaaa tgattttcaa gagttgctga attcgtgtgg aacgatctgt attgattgtt
    24121 tccattttac ctgtcttggt gtttagttta acactgatta tcccatcatc aacaaagtct
    24181 tttttattgt atttgattaa ctcttccata atgattgagt ctccacccgg gtcaggcttt
    24241 cgtttgatta aatctgtttt acgaatttgt ctgactttat aggattcact cgaaatcaca
    24301 actcgaaaca attcgtctgg gtttttagag tctttttgga atagttctgg atctttagat
    24361 gtgatttcta cttcatttcc attttcgtct aggatctttt ccttaggtcc tgctggataa
    24421 aaagttttta ggtcttttgt gtctttgact tcttcttttg tgctagagca agagaagaga
    24481 atcgcagaag aaaatagtaa aagtagtcct gtaatactcg ccttattttt tagaatagaa
    24541 ttttgcataa cttgtccttg gtgttttaga tagaagcttt ttgatttttt actactacgt
    24601 caagagaaaa gagaatttgt ttatcctaca tcaatccaac acatccttac ccagatcttt
    24661 taataaatta ctaccgatgg caccgataaa agatatgcta attcaaaatt atcaatcgtt
    24721 tctgttcgtt cagttatcgc tggtaattct taagacaaca ttgagcgaag ggtggaatga
    24781 ataccgatat aaatgaataa atttaaaacc atcggtgttc atccgtgttt atcggtggta
    24841 atcagtttta acttacttgt gagtaaagat aagatcaacc caccggattt aacttttgtt
    24901 ttaattctcc tagcttctga ttttctggat cgagtctaga aattagaata tacaatttat
    24961 aggcacgaga agtcactttt aattctgtat aaacttctgc tagaaaaatt aaattattca
    25021 cattcttagg ttctcttagt ttcaaacgct ccgcgtaatc cgctgcaact tctagatctc
    25081 caatcatacg atagcttttt gcagcggcat acaagaagtt agactcttct ggatataaat
    25141 cgataaatgt tgtataaagc tttgctgctt gtttataatc accgagtcta taagtcgtat
    25201 ttgacattag ccgcaaataa gattttgtat tagaaggttc tatttgtaaa agaatctcat
    25261 atgctttgcg aaattcttct ttattataat attcgagtgc taatttaatt tgggaattat
    25321 ctacttccgt ttcaatttga gaattatcat tgtattcaat tctgataaga ctgaagtcat
    25381 ctgtgatttc tcctttcttt tccgtaagtt caaatatttt ttctagattt ccgtctgcct
    25441 gctcaactgt agcaagaaaa agactttcat cttcgtttat aattctacca cctttatcat
    25501 caatgcctat acgtatatcg tcttttccat ctgaacccat aaaaatcaca tccgaatttt
    25561 ctaatttgaa tgtgctaatt ttaaattcac ctgaatgctc agaaagaacc attccaattt
    25621 tatgcaagct taattctttt tcaataaacg atgcctttcc atcccgatag agaacacaaa
    25681 atggatgctc tgcattgata taatataaaa aaccacgatc gttatccacc aaacccatta
    25741 caacagaaac catcatggat ccatcaaagg tctcaaaaat actttgcaat tcttgaaagg
    25801 ctaattttaa ccatctctct ggaaaaacag attcttcttt atcaaatatt tttgctctag
    25861 ataaaatgga tttaaatacg actcctaatg caagagcgcc tcccgcacct tggatcgatt
    25921 ttcccatcgc atctccgttt acaaaagcaa tgtatttctt tccttttaaa tagatgattt
    25981 cagaaataca aatatctcct ccaatatgat gggttctatt tttaaattca aatttctttt
    26041 tttgtcggat caaagtttta aaagtaactt tttcatctcc agaaacgtta gatgttaaag
    26101 gctgaatgat taaagtagtt agaaaataat ctccatcttg gtgttctttt aagagcttta
    26161 cttcttgcag ggtctcattt aattctttgg ttctttcttc tactttactt tctaggtttt
    26221 tatttaaatc ttcaatttca atatgaacag aaataaaacg gtttgcgaga acagccgcga
    26281 atcctagaat ataaataaaa aatgcatatt tagagaaaga aagccccgaa ttaaatatga
    26341 gcatatatca tagatagccg ccattataaa aataaccaat cctacaaata atcgctttgc
    26401 attttggaca ctgcttttga ttgcatgata aaagattcta ccaattaaag gaataccgat
    26461 tgttaatata gaaatttgcc acagccgtaa aatatactct gcttgataca tctcggggca
    26521 aatgatcgtt agcccaccaa tgatataaga aaaataactg tagaatcgag ttgcctttct
    26581 agattttcca aaaaataaaa catctaggga ttgagacaaa aatgaaaatc ctggatacaa
    26641 aacagcaagc tccactcttt gtatgattgt agtatcccac gcattctcaa aaattgcaga
    26701 acttcttgta taaatataaa ctccaacgat aaacgaaaag gctccataat aaaaattata
    26761 aagctctact cttctacgat agtagagaaa tagatggtag agtcctacaa tcatataaac
    26821 agtcatgagt gcaagactca ctctatcctg ctcatcgtat tttatattat cataaaaacc
    26881 aaaatcataa tatcttgtca gataaaaacc agtatgatcg aatttaggat gaccggataa
    26941 ttttactaga agctgattgt gacttgcacg catcagctct cttggaactt gataaacaac
    27001 tcctctgacc gttctatggt aagaaacttt tccatctttc acttctcctt cttttgcaat
    27061 caaagttcca ttgaaataaa tctcgaacac ttctccaatt tcaccaaaac gaattccaat
    27121 atgtttcgga taatttaaaa tatcctctgg aatatcaaaa taggtaagaa gagtgtatgt
    27181 tgcaagagag ccctcctctt gataaaattt atttagccaa attggaaaag cttctacctt
    27241 cttccatttt tcatctggct cttctaattg aaggaaagaa gagtcatagc tatttataat
    27301 ataccaagct tgcggattac aaatgttatt tgaacaagac ttagttagat cgattcgatc
    27361 caatggaaaa actgagaatg gtagaaagaa aataaggagt agatatttca ttcgattaaa
    27421 gttcctgaga aataaggtaa gcataaaaat aatacttaaa atattttata attgtaaaat
    27481 caaaaagtaa atatttttca aaatcgaaag acttttcttt ttatagatca agtaaattcg
    27541 cataacacca tatatcctct atatttaaga tatatacttc gggatatccc taaattagaa
    27601 taagattttc taataggcaa agattcgtcc ctttttgaat agtattaaga ggggatgtgt
    27661 attcattcga atcctgagaa aattcatttc tatgtgacaa aatcttacac agaacccaaa
    27721 aaaaccaaac aagtaggaat taataatgta tcaacataac ataaaagaga atctaaatac
    27781 aaagtttaaa atcgtaatga tttttatatt acttttcaat ttaagctgcg cgaccttact
    27841 cccaacggaa aagcgaatct attcagaaac agtcgaatat aaaaaaagtc aagactcatg
    27901 gaaaaaagca gtctccagtt gggtttctac acgcttcggc gaacgaggat tatccacaaa
    27961 gaaagaagag aaagacattt taagttttgt tttaggaatg ggctatgacc ctaaaaaaac
    28021 attaccgacc gttggaagtt gtcactttat tttaaatgta gagtatgcga ataacgcact
    28081 caatttacac tttgaaaata ttttctttgt gaatggatat ggtagagtgg gaactacagc
    28141 tcgtatgcac gatggggaaa gtggtcctaa caacgaacaa gagttaaatt ccatcattga
    28201 aaaatgtatc aaagttgtgg caacagattt gaaagattcg ataaagtaat ttaagccagt
    28261 tcgatgtaac agaaaaatta aaagattacc accgatacac acagatggac acggataaaa
    28321 tattaagaat tattaaaacc atccgtgttt atctgtgttc attcgtggtg aaaaatcctt
    28381 aacttgacag cattacctgt cggcaacttg ggctagccgc ccaaaccctc tgattttttg
    28441 atccattaat aaaccataat ataataattt ttttaatcat atttatcatt tcctatctat
    28501 taatatcagt tactaatttt tattacatag tatactagat agaataacgt ctactctttt
    28561 gataaacgca cttggaataa aaataaaacc catcggtgtt catcagtggt taaaatgaat
    28621 tcaccttatt ttagattgaa agaatctact tcttgataag gattattcgg atacttatag
    28681 ttagctgaag atttaaagta ttcgattgga gtctctccaa ttttttcata gacaccttct
    28741 gttttaaaat ttcgagaatg aaaaattaat tcatagcgat aattatgaat cgatactata
    28801 gaggcactta cgaaaaaaaa gattcctaaa atagaagcga taggtgttaa tagaaaataa
    28861 tctctcgatt tctcataaat ataattccaa aaaagactaa aataaatagt aaaaagagaa
    28921 gcaggtagaa tccaaaactc aaaataatgt ggctctatat ctattcctat aaatagaaaa
    28981 gatagaaaat ttaaggttaa aatgaaaaag tttctgccat agaatcgaaa caaagaatta
    29041 aaaaagaaaa gtagaatgaa taaaaagaat agaatgaaaa tcggaagcaa attgtgagta
    29101 accacaactg gatcattcca ttgattgaat tgaaataaa
//
LOCUS       DAOQOS010000067        28973 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_730_length_28973_cov_6.674320, whole genome shotgun sequence.
ACCESSION   DAOQOS010000067 DAOQOS010000000
VERSION     DAOQOS010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 28973)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28973)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28973
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_730_length_28973_cov_6.674320"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..910)
                     /locus_tag="PLF66_14945"
     CDS             complement(<1..910)
                     /locus_tag="PLF66_14945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004438298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HMZ65392.1"
                     /translation="MQEYKKKIEEEQKVATKKKAMIPNLSRLLGNLAVYYFVPSELSF
                     FHWIILFICLYSSFWFVLELLIVLDYRKYKFLPYIPATSDLISAFVLIYLTGSGNSAF
                     IVAVISFIVVSMLFSSDTHQSIYLVIMSSSLYILLLVGLYFEVYPYVNLLNFQNNKNL
                     ANYIFSFGLLFIILVGMFNSVRMISKANVELNEKLKQLIEVSDREKNRSESLLLNILP
                     FEIAEELKDKGIVQPVFYESVTILFTDFVGFTIIAEHMSPEELIKTLDASFTKFDKII
                     ERYKLEKLKTIGDSYMCAGGLPIRNRT"
     gene            1237..1494
                     /locus_tag="PLF66_14950"
     CDS             1237..1494
                     /locus_tag="PLF66_14950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65393.1"
                     /translation="MNPMNIRSTYAFDEETIQIIKSFSKEWKVSQAEVIRRSVRIALQ
                     LERKVKTPMQVLNNLDKKPPLTKKQAKSIQSKIKQSKLAWD"
     gene            1495..1875
                     /locus_tag="PLF66_14955"
     CDS             1495..1875
                     /locus_tag="PLF66_14955"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HMZ65394.1"
                     /translation="MRKIHLDTNFLIELHVIGSKSRSILKSLLKEKVELSISAITWHE
                     FLCGPLDSEYQENILNLVEKRIVAVSADVAELGAILFNASGRKKGSKADCLIAATSIY
                     SHAELLTWNLSDFKNFQQFGLLLV"
     gene            complement(2103..2372)
                     /locus_tag="PLF66_14960"
     CDS             complement(2103..2372)
                     /locus_tag="PLF66_14960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65395.1"
                     /translation="MVVVNKQTLIHWMLLDSLAKENYYREKIQIFQKKYNVTFEAFEN
                     KIETAEKESFEEWDDFIEWEAYEGFLSRLKERIHDIRNGNIQLAG"
     gene            complement(2485..2712)
                     /locus_tag="PLF66_14965"
     CDS             complement(2485..2712)
                     /locus_tag="PLF66_14965"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HMZ65396.1"
                     /translation="MKNSTYTDEYQKFLRLLKKARKEAKLTQVEVADILHQPQSYVSK
                     CESGEKRVDIIEVCKFAKIYKKPIEYFVKGI"
     gene            2830..3516
                     /locus_tag="PLF66_14970"
     CDS             2830..3516
                     /locus_tag="PLF66_14970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65397.1"
                     /translation="MSKYFEIKKEVGIQIDKISEPMKITFGTGWQIDIKYRYKCPYCQ
                     TLDNLHKSIRANNDGEHLLINTSDTCFNCKASYKPQLILKCDFSELRKEEKIKSKSVE
                     IQHDAETSESNINLANDSNSKKQEAGFIKRLIVASFKCTYYLFADRKGNIIMGIVVII
                     GLIARLFSDKHEEIPLGLATLLVIIAIAGFRNHKIYKSSELKFHQQLGEDCLAGLRDM
                     FNIGLNEKVF"
     gene            3529..4665
                     /locus_tag="PLF66_14975"
     CDS             3529..4665
                     /locus_tag="PLF66_14975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65398.1"
                     /translation="MSKSLRNFFNRRSVKYFAFAIISYFLFIVIGIKAISYISPSDLK
                     FWENIVPIAGLFFSGILGFILDKEKEQGLKLYEKKIEACQDFLKVLEDVIEGGIILLN
                     RDITSKKDELNKIFFAVSKLRMHFEEKDILEIIGKCTDISDALNERRKNNKNEKDYYQ
                     KLSSALFGISKIFKELLEQLKPEGQKENLKEPNQFVIAIDEFILNVIQPTDQSHVSEN
                     TWHVNVGEDYNIADPKDQYRCWQDMLNFGFWSAGGAKRYADGTKKLKEGDTIYAYLSG
                     KGYIARGDVKEKAKLVRDFFSGKDKNKIISKSFLEHLNDKSFEGFHNADIGEYAVKVE
                     WNFKNPPVTNSDLHISPMTICKMNQANLAILEREFQTKKGEIKK"
     gene            4662..5576
                     /locus_tag="PLF66_14980"
     CDS             4662..5576
                     /locus_tag="PLF66_14980"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HMZ65399.1"
                     /translation="MKKLLFNILLILNIIFINCVSVEERTNQLNHAARYGQKDQVDQL
                     IKDGAVLSKDTLNSAIISGNIEVVKFLLSKGLDINGSDRNKMDSLTYSFESPKSQKEE
                     MFKFLLAEGAKMENPYNILYLGLRDMITPAVTAAIESGAYVDNSLVFEAGTPEHLEIL
                     LKNGANINAQNSSGYTLLHINIICCSPRPISSKFLIEKGINVNLKNNYGQTALHIAIK
                     EVDRVKFLLEQKNIDINARDNDNATPLHYIWDADTAKILLSKKGINKNPIDKFGETPY
                     QKVRREVLERETDRNKALLYTLKQAGCH"
     gene            complement(5581..6045)
                     /locus_tag="PLF66_14985"
     CDS             complement(5581..6045)
                     /locus_tag="PLF66_14985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65400.1"
                     /translation="MEDLKNKLYKLMWVPETKEIIWDMQTEEAIPADKGIYKEVNSIF
                     NLEDWSEEFRQIYSAWLEEDKDELYDIKNAFYQKTLNNIEQVNKKAKDYHLYLWFDID
                     RTLNENFIWNTCPITLTPLICLGDNYHYLNRFISKDKFLVLPDMEQVVVNRR"
     gene            complement(6140..6346)
                     /locus_tag="PLF66_14990"
     CDS             complement(6140..6346)
                     /locus_tag="PLF66_14990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65401.1"
                     /translation="MERILNLEKKLFFLALALLLVVTFNFMPSLEILIPPIVVSNDSV
                     NETADGIISKNRKGSIRAEFPSEF"
     gene            6445..6585
                     /locus_tag="PLF66_14995"
     CDS             6445..6585
                     /locus_tag="PLF66_14995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65402.1"
                     /translation="MKRQKRTKGAIVEIPLNNGYHTYGRVLKSGMAFYDLKSNLRDKK
                     AS"
     gene            6709..6972
                     /locus_tag="PLF66_15000"
     CDS             6709..6972
                     /locus_tag="PLF66_15000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65403.1"
                     /translation="MQTLEIQIQNDGDYQLFLSLANRLNCKVIKKPALKKKEWQLTNE
                     MKSELDARLLAHEKNPEEGYSMADVKKELEKRLGRKIQVKARG"
     gene            6938..7255
                     /locus_tag="PLF66_15005"
     CDS             6938..7255
                     /locus_tag="PLF66_15005"
                     /inference="COORDINATES: protein motif:HMM:NF016876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HMZ65404.1"
                     /translation="MGEKYKLKPEAKKDLVQAVEWYEKNQEGVGLRLYEEVYDKIEAL
                     SKKPNSHSTFYREFRKASLKVFPFLIIYIIKEPIILIFAIWHKSRDSKKLLERVDRTE
                     KDK"
     gene            complement(7363..10269)
                     /gene="gcvP"
                     /locus_tag="PLF66_15010"
     CDS             complement(7363..10269)
                     /gene="gcvP"
                     /locus_tag="PLF66_15010"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010577155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HMZ65405.1"
                     /translation="MKMTMISSSVASEKSSNPLTPADTFLKRHVGSDSKEVKEMLATL
                     GYESLDAMINTAVPANIRLKNPIQVTEAKSEYEVLQELKRIFSKNKIFKNYIGTGFND
                     CIVPAVIQRNILENPGWYTAYTPYQAEIAQGRLEALLNFQTMIMDMTGLEIANASLLD
                     ESTAAAEAITLCHAVKSDENGDSIFISDECHPQTIDVVRTRAIPVGFNVVVGDYKKFK
                     ADKSFFAVLIQYPATNGNVYDYEELIKQAHANGVLVVTAADLMSLAILKSPGELGADV
                     AVGSTQRFGLPLGFGGPHAGYLATKEEYKRSLPGRLIGVSKDSQGAPAMRLSLQTREQ
                     HIRRDKATSNICTAQVLLAVLSSMYAVYHGPEGIRHIATRIHLFTSILAKGIESLGFK
                     LNSKEFFDTLSIAVDKTKQSEIRKIAESKNVNLRYEENWIGISIDETVGKEDILVLLS
                     IFNQGNAVNVHIAADTVAYNFSEKLVRKSEYLTHPVFNTYHTETKLLRYIRKLESRDV
                     SLTHSMIPLGSCTMKLNASTEMLPLTWPEVSKLHPFAPIDQTLGYREMFSQLEKWLCE
                     ITGFKAISLQPNAGSQGEYAGLLAIRDYHLSKNQGHRTVCLIPTSAHGTNPASAVMAG
                     FKVVVTACDENGNIDVSDLKEKAEKYKNELGALMITYPSTHGVFEATIKEICEIIHTN
                     GGQVYMDGANMNAQVGLTSPGEIGADVCHLNLHKTFCIPHGGGGPGVGPIGVASHLVP
                     FLPGHALVENGSKRESNSVTSAPWGSASITTISWIYIAMLGGEGLTYATKMAILNANY
                     IAKRLESYFPVLYRGNKNLVAHECIIDVRRFKATAKIEVDDIAKRLIDFGFHAPTMSW
                     PVPGTLMIEPTESESKEELDRFCEAMIQIHREITDIENKKLDEIDNPLKNSPHTAKMV
                     MSDKWDHLYSRELAAYPLPYLKEHKYWPVVGRVDNVYGDRNLVCACLPIDSYNE"
     gene            10811..11149
                     /locus_tag="PLF66_15015"
     CDS             10811..11149
                     /locus_tag="PLF66_15015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020776951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HMZ65406.1"
                     /translation="MAQNKKSEFKEKIQNLSELAKSISHPARISILKTIAQKQECICG
                     EIVEVLPLAQSTVSQHLKELKEIGLIKGEVEGVKSCYCINWKKVEEFEKEINKLFQEL
                     YTFKNKDYCC"
     gene            11267..11680
                     /locus_tag="PLF66_15020"
     CDS             11267..11680
                     /locus_tag="PLF66_15020"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010575515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HMZ65407.1"
                     /translation="MKKILVLCTGNSCRSQMAEGWLRHFANNKAVIYSAGIETHGVNP
                     KAIQFMKEVGIDISNHTSNHINEYKDIDFDFILTVCDHAKENCPYFPTNALRLHHNFV
                     DPSKVIGTEEEIANAFRMTRDEIRDYCKNFIKEIE"
     gene            11677..12687
                     /gene="arsB"
                     /locus_tag="PLF66_15025"
     CDS             11677..12687
                     /gene="arsB"
                     /locus_tag="PLF66_15025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HMZ65408.1"
                     /translation="MSFFERFLTIWVILCIIFGVLLGQIAGDWVSKLTNMSLFEVNIP
                     IAVLVWLMIYPMMVQIDFSSIKDVGKNPKGLILTLVVNWLIKPFTMALFGWIFFLNLF
                     STFLPADRANEYLAGAILLGAAPCTAMVFVWSYLTKGDPLYTLVQVSINDLVILFAFI
                     PIVGFLLGISNISIPYNTLIYSVVIFVVIPLSAGYVSRKYIINAYGKDWFETKFLNTL
                     KPVSILALLATLVLLFAFQGSIILKNPFDILLIAIPLTIQTYFIFGISWAMGKKLNLP
                     HNVCAPASMIGASNFFELSVAVAIALFGVNSGAALATVVGVLIEVPVMLTLVKMANRW
                     RF"
     gene            complement(12684..13178)
                     /locus_tag="PLF66_15030"
     CDS             complement(12684..13178)
                     /locus_tag="PLF66_15030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65409.1"
                     /translation="MKKLYLLMNLVWFSCLNTNYNLENLKTTKEELFKEAVFYATRND
                     SKSMDKIILNRKEHNEFFWKHVGERFTSDPGMTPDLAYDHMSLETRIAWRELIRDLNG
                     KNLEFSKVECTAPSEVYGPFTLIRGCISYFRNLDTDEMIQIKKIRNIIQYNGKVKIYN
                     LKRD"
     gene            complement(13273..15888)
                     /locus_tag="PLF66_15035"
     CDS             complement(13273..15888)
                     /locus_tag="PLF66_15035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65410.1"
                     /translation="MKKKLFISILMSISVFQFSCEYIRQSKEKPSALSRLMVSYPPKP
                     RDPGLYISGTILDSTGKAIPNATVQILGRGESSVSILPPASVRTIGRPALPPPSTNPN
                     GFTTLSAVLSDKVGWGSTATSCPSSATAAAYTTQGQLTSVSFNGTPGGCILGFNNTTG
                     AVNSTVSNSAPNCSAPTGAGGLVGSNGVGACNFFGSTNSIPNGYYFDNSQGASATAID
                     GSACPGTNFDRTATNNGNATGCPATFIAPHSELSAANLSSGNLSKGAQYVLQTCGRTV
                     VDAVNSFGTTAPLSTNPDSFTGFGQNVRVYCDTSTLSSAATSVGISNTVVPRNTVVAS
                     FTTDATGSFNGIKFTVPNVGNTYDIKVTTSAGRVLKTRRLKINNNGVSNVLQPVTEAI
                     SRALLESSTGKDITSTESSWSNIAASQVDGTSSPSSVPSGSYDFIPTNISIEIAVPRN
                     YIGILSGTITSDVVWTRTDGQYYLNGTVIVPSGRTITIGPGVTVIGSASPGGALLIKP
                     GGKIFVNGTANAPVVFTSEKSAGSRAPADWQGIIIQGNGVQTFGGAGSSAIGEGDVGN
                     YGGSNNSDSSGSINYLRVEFAGAPFSPGNERNCISLMAVGSGTSLNYVQCHRGFDDGF
                     EQWGGAFNARYLISTGNRDDQFDFADGFIGTYQFAIGQIYASTAPTNDDITRCIEGDG
                     NSALSCTSSARNGGDCSDPYFANVTCVGAGGSTGGNGALGGPVTNHGDAIFVRRATPT
                     FQLGDYTNILVDNYGNNKAVGCRTDSRTVDARITNLFYTRSATSDCPTPVTNLATPSA
                     GISTQVVTTISETSPDFTPLATSTPTISATDTITVTNFTNTSGASGFRSAAYYGAVRD
                     ANDTWWKGWTSFSQN"
     gene            complement(15912..18908)
                     /locus_tag="PLF66_15040"
     CDS             complement(15912..18908)
                     /locus_tag="PLF66_15040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016544723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HMZ65411.1"
                     /translation="MKKQINFLILLLMLVSSAVFGQAKTGAGSIQGDVLDAQTAEPMF
                     GVTAVIRSISKSARTDLDGKFTITGVPDGEYDIEFIMQGMDTQKRKISVSGGKPAKIN
                     VAMGIKKLDTVVVEGRAMNDTESSLLKLQKKASTVSDGISAQAIAKTPDSSAGDAARR
                     ITGITLVGGKYVFVRGLGERYSNTLLNGVPLPSPEPNKRVVPLDLFPSALIKNIIVSK
                     TFIPEDSAEFAGGTVKIETKDYPDKFFLKVGFSMGYNNNTTGKQFQTYSGGGQDWLGL
                     TAGNRERPDIVSTLPGVQFNAVGPGSFGYNSGAITAGSLAFNNQWSSNTTVASPNKGF
                     NISTGNVFNVANRKLGVIFALTYGNDLQFREERDVTNRVGGIVPRAPVGGKNAYLIKY
                     YDYNQKIWNESVNWGSAFNTTYEIANGQRVHWKNFFSVNNDKEVWNYGGSSFASGQQI
                     DATKLNYIMRNLYNTQLGGDHIIKIADKFTKLDWKVSQSEAFRNQPDMRDSYYQVPLT
                     TDPATNSQIPQYNAGAGLAESQLSKSRFFSRTLDINRYISFDYEIPFTQWGGLQSKFK
                     VGYSYLTRERGFDAQRYYFRKADPGTNLGGGALTPAVYPVPPEAVLNPFNRGVQGYYV
                     SESTRPTDTYEARQKLTSKYAQVDMPLTPKLRFIGGVRNENNYQVVSTVNPFDRLASF
                     AANYDFKTYAITNEFQQNLIDPTYRQPRAINKNTNTLPSANFVYSLDDRTNIRLAYTE
                     TITRPDFREMSSFEFTNILGGPPEKGNPYLKRSYIHNYDLRYEFYPKGDDLISFGVFR
                     KNLSSPIEKVMEVDSQFRYTYTNAKSAYIQGAEFEARKGLDFISEKLEKWSVGFNMFL
                     IKSEVTFNDWLYYQLSPNDPKRPTNLSRPLQGQSPYVYNVNVRYKLDKVGDHTITVLY
                     NEFGKRINAVGGIGIPDTYERPVGMLDMVYNLKWKDKWDFKLAARNLTDTRIKIVQED
                     PWRGKDTTINSYRLGPTITFSASYNF"
     gene            19220..21136
                     /locus_tag="PLF66_15045"
     CDS             19220..21136
                     /locus_tag="PLF66_15045"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HMZ65412.1"
                     /translation="MIKNKIQKIYAAKSSLRAKLFLGFIILSLVLSSVLGFTLYQVSS
                     SVYYKSFLQHKKSLGISISSSIDGEIFQSFITEKSSETPEFKRYFNFMQRVSANEEYI
                     TWIYSVLIDKKTNKLIYAIDATHISEDTIWIENMFIGLKVYINRNGRLVVYWDSIEHV
                     EDFQIVNKGKKFEIEIDSLNETVSINKIQILKVTKKNPFTVETKKGILNKENPAQTAT
                     IKVEEESLDVSLNFAEKNSQSSIPGWEFIETEDLVNKIKGSINTCTFHMQNEPEQLAY
                     GTFNIVIAPIFKSHTECIGAVIFSVSISDIRQFNKTMMIAGFTVTIIALILSVIVSYI
                     LSIFFTDPLHKLSQAVDELSSGNMETNVIIQSKDEFGFLSEKFNEMVANLKKAYKEKE
                     SLAALRHELNVAQKIQTAILPKSIPNLPNLDIAVNYYPMTQIGGDYYDFHMIDKNKIG
                     IFIADVSGHGIPAAIIASMLKIAFSIESAFAEDPSRLLSRINQSMLNKVGTNFISASY
                     IYIDLETNKIKHAKAGHPSIYIHSPETDEVKEFNQKGKIIGLFPEIQTETVEISIQKG
                     DRIILYTDGIIEARNSKEEMFGEELFVGYIRKHNSMKPADFARLVMNDVKSWIGNNAS
                     KHSDDVTLMVVDIK"
     gene            complement(21173..22135)
                     /locus_tag="PLF66_15050"
     CDS             complement(21173..22135)
                     /locus_tag="PLF66_15050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004787585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HMZ65413.1"
                     /translation="MKQILIILGIVAMISCQSTQEIREATKTVEESQVIKEETKETSK
                     TIATPKYDEIGFASWYGIQFQGKVTASGEVFDRFKMTAAHRTIPFGSVVKVQNLENNK
                     EADVTINDRGPADEGSIIEVTEKAAEILNFKEESVAKVGINIIKPMEENPILVKDDSF
                     SVDDDDDDDDDDDIDEETAPPAKKPDAKKPQTVTPVAPDKKPAPAPAPAPVKTAPANN
                     SKTTAPAEIPTVNVPTPTTSNTNNASQPKGETVQIGVFKEQRRAEAFRDQIKKDFSEK
                     IFLFSRNGTYVVQIGDFAKREDAVALREKLKAKKIANCFIPPKN"
     gene            22471..23457
                     /locus_tag="PLF66_15055"
     CDS             22471..23457
                     /locus_tag="PLF66_15055"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65414.1"
                     /translation="MKNRSDTMTPSNFSGYVNTIKENVKRKYSHYKVNKVRQHYRDGL
                     SFEDNAEYSKAIESYLECLKLDPDYIKCLKRLAKLYSNLEEHSNAIQTYTKIAKLQPN
                     EENFFELGQEYYKQNRFKKAILCLKKSLYYKRRFIQSHRLLASIYARVENRDKTEQYL
                     SNVIKIDPNHKGSMEELIKLYFIQGRYRDSLNLLEKYKEVYVEDSNIKFLKSDIYVKM
                     GKYSKALNIAYESLIEEEGFQSFIEDMQYKKVNPTLKEKDFLRKIVLIKNEKKKLLEE
                     NLKSYQSGDETKFPDPKISYDLALLYLLLGNQKKAMKYLFFARQLNEERKYI"
     gene            23473..24480
                     /locus_tag="PLF66_15060"
     CDS             23473..24480
                     /locus_tag="PLF66_15060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65415.1"
                     /translation="MHITFLFLIIFVLNNCFSIFRPTPVLEDDKSDILKADKQFILLD
                     DNLKPIEKPDFTGLDKKVKALQSAKTFHDLNNLAVYGVKLSYIESSEEILRKLQKEKP
                     TEQVPFLNLLRIYYLLEEYDIAKKMLNDYYKANIQDKKKVFDFMKYLKSTNRMEEHVL
                     FLDVISSYQEHEMQALEELGNYFLSIGELELAKSYFEKILTIYAYNPIALQSMMQIHY
                     TNEAWTDVITYAAPLRKQKTKEKNYHTMLAKAYFELGDYAQSLKISEEAPEQEKSSID
                     FLVVWRDSILCNDLKAPLTKLNKYFQIAQKKNPTLKESEFFLASSKEGRQMISNIIHG
                     Y"
     gene            24484..25461
                     /locus_tag="PLF66_15065"
     CDS             24484..25461
                     /locus_tag="PLF66_15065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004456970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HMZ65416.1"
                     /translation="MKEKILFILFCLLIASCGPNERIKALVRNANEIFGVIPDKMPGS
                     ENDTLAIINLGRKLYFDNRLSVNDNQSCNTCHNVLNKRAGVDNLNFSPGVFGKNGNRN
                     TPTVLNAGFQIAQFWDGRAKKLEDQTRIPLFNPLQMAIPDERYLVDKIASIPEYKDLF
                     EKAYGDKSGKITFDQITNSISAFQRTFKTNDRFDDFQRGKYKALNPEEIRGLEIFLSV
                     GCANCHKGPLLGGNSFQKLGVKKQYENKEDLGRTKVTGKEEDLFVFKVPMLRNVALTA
                     PYFHDGSINTLDLAVKKMADIQLGKSLSEQEIEYIVAFLNALTDKSREN"
     gene            25463..26272
                     /locus_tag="PLF66_15070"
     CDS             25463..26272
                     /locus_tag="PLF66_15070"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HMZ65417.1"
                     /translation="MEVIKRNNISIKGEGKKVMLFAHGYGCDQNMWRFITPEFEKDYQ
                     VILFDHIGSGKSDLGYYNKNKYAKLHGYANDILDICDALNLKNVILVGHSVSTMIALL
                     AANKNKSLFSHIIMICPSPCYINEENYIGGFSKEDILEMLESLESNYLGWSSSITPVI
                     MGNPERPELTEELKNSFCRQDPEIAKHFARATFLGDYRKDLEECMTPTLIIQCSNDAI
                     APKEVGDYTNKHIHKSEMVTIEATGHCPHLSHPTETIQAIKNYLAVNLTDE"
     gene            26269..28203
                     /locus_tag="PLF66_15075"
     CDS             26269..28203
                     /locus_tag="PLF66_15075"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HMZ65418.1"
                     /translation="MIENDALKIENCLNDAPCGYLRLDSNGRIFFANNTLLKWLNFEL
                     DNLTNKVWTFLLSGGSRIYEQTHFTPLLRMQGYVEEINLDLIDSKGDKIPFLINAKIL
                     VEDSTVDIFLFRFNQRKSYEKEILIAKKNAELSAKVKSEFLSNMSHEIRTPLNGIIGF
                     SELLLSSKLDSSQEEYIRIINQAGNSLLELINDILDLSKLEAEKVELHIEKTNVYQLL
                     EHCIDLIKFKANEKEIELLLYISPNVPEYIFTDPIRLRQVIINLLGNAVKFTEKGEIE
                     LRVEGNITNLNSNQIEISFTIRDTGIGISNEKQRVIFDAFSQADSSISQKYGGTGLGL
                     TISNKLLKLFNSKLNVNSQPGKGSEFYFTVSSHFEINPNSEKDLYLDSFSALILDQND
                     RSRTIISKMLDDLRIKNKSSANIELALSSLDSNVFNLLIINNNLTSFSEFLNKSNNFN
                     VYNDSKFTTILLLESTVADWNEDFQYPSNIVKIFKPLRKKDLVDVLINRFPNIVRKSL
                     IKQQETLYSMVNENLKIMIVDDDEVNLFLAKTVLDLVMPKAHIVQFNNGLEALKNIEK
                     ENPNIIFLDLQMPELNGIETTIEIRKINSFKEVPIIAFSAGMIPEDLEKCKDAGMNDY
                     LTKPVKKEDFQRVLEKWLSK"
     gene            28376..28960
                     /gene="msrA"
                     /locus_tag="PLF66_15080"
     CDS             28376..28960
                     /gene="msrA"
                     /locus_tag="PLF66_15080"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016549689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HMZ65419.1"
                     /translation="MLYRIILSLFIICLPLTAQEKVEVAVFAGGCFWCMESPFEKING
                     VKEVISGYTGGKKENPTYEEVSSGLTGHLEAVQVYYDPKKISYIQLLEIFWRQIDPTD
                     PDGQFVDRGKQYRTAIFYKTNEEKTQAERSKKNLDKSGKFSSPIVTEILPLIKFYPAE
                     EYHQDFYKKNPVRYNYYRYGSGRDQFLEKIWDKK"
BASE COUNT        10007 a         4939 c         4753 g         9274 t
ORIGIN      
        1 gagttctatt ccgtattggt aatccacctg cgcacatata actgtcgcct atcgttttta
       61 atttttccaa tttatatctt tctataattt tatcgaattt agtaaagcta gcgtccaatg
      121 ttttaatcaa ttcttctgga gacatgtgtt ctgcgattat tgtaaatcca acaaagtcgg
      181 taaataaaat cgtaacactc tcataaaaaa cgggttgcac aattcctttg tcttttaact
      241 cttctgcaat ctcaaatggt agaatattca aaagaagact ttcgcttcta tttttttctc
      301 tatccgaaac ctctattagt tgttttaatt tctcatttaa ctctacattt gcctttgata
      361 tcattcgaac agaattaaac attccaacta gaatgataaa taacaaacca aaggaaaaaa
      421 tgtaattcgc taagtttttg ttgttttgaa agttgagtaa atttacataa ggatagactt
      481 caaaataaag acctactaaa agtaaaatat aaagagagga agacatgata actaaataaa
      541 tggactgatg agtatcagaa gaaaaaagca tactcacaac aataaaagaa ataacagcaa
      601 cgataaacgc tgagttgccg ctacctgtaa gataaatcaa aacaaaagca gaaattaaat
      661 cagatgtagc aggtatatag ggtaagaatt tatattttct ataatctaat acaattaata
      721 attctaaaac aaaccagaaa gatgagtata aacaaataaa caaaattatc cagtggaaga
      781 aacttaattc acttggaaca aaataataaa cagctaaatt gccaagaagc ctggataagt
      841 ttggaatcat tgctttcttt tttgtagcaa ctttctgttc ttcttcgatt ttctttttat
      901 actcttgcat atctgaatca atatattttt ttatagatat ttattgtcga gtaatagtta
      961 gattttgaat aggaattgaa attagaaata tgaatcttgg ctcattgctt tcgctaattt
     1021 gttatttagt ttaggaaaat agaataaaag tgacagtaga acttgctctc tcaggctaat
     1081 aaaaataagc cgctgattga aatggattat gaactcaatc ctatcgacca actttttatc
     1141 tgaattatat ttggagatgt atggctaatc tcaaatttca attttttttg tataaaaata
     1201 atttgacttt tattcatacc atataacgct tatggtatga atccaatgaa tattcggtcg
     1261 acttacgctt tcgatgaaga aactattcaa attatcaaaa gtttttccaa ggaatggaaa
     1321 gtttcccaag cagaggttat tcgtcgttct gtgagaattg ccctccaatt agaaagaaag
     1381 gttaaaactc ccatgcaagt tttaaataat ttagacaaga aaccgcctct tacaaaaaaa
     1441 caagcaaaga gtatccaaag caaaatcaaa caaagtaaac tcgcttggga ttaaatgcgc
     1501 aaaattcatt tagatacaaa ttttttgata gagcttcatg tcattggaag caagagtcgc
     1561 tctatattaa agtctctgct gaaagaaaaa gtcgaacttt ctatcagtgc cattacttgg
     1621 catgaatttt tgtgcggacc tttagatagc gaataccaag aaaacatttt gaatttagta
     1681 gagaaaagaa ttgtagcagt ctcggctgac gttgcagagt taggagcgat tttattcaat
     1741 gctagcggtc gcaaaaaagg atccaaggca gattgcctaa tagcggctac ttccatttat
     1801 tcgcacgctg aacttttaac ttggaatcta tcggatttta aaaacttcca acaatttgga
     1861 ttgttgctag tttgatttag actttgtttg ttaaaggaaa agcaaaacac gtcaacgatt
     1921 ctacttttaa agtaatcgaa ctattacaag atgaaaattt tgaagcaaac cttactacta
     1981 tcaaccatat tgtgagaaaa aattctaatt ttttgtatcc gttaaccgcc gaggaaggtt
     2041 aggacttaaa aaacttttta aaaataattt ctaatacttg ataatttgaa acaatttcac
     2101 tgttatccag ccaattgaat atttccgttt cggatgtcat gaatcctttc ctttaagcga
     2161 cttaaaaaac cctcgtatgc ttcccattct ataaaatcat cccattcttc gaaagattct
     2221 ttttcggcag tttcgatttt attttcaaag gcttcaaaag taacgttgta ttttttttga
     2281 aaaatttgaa ttttctcacg ataataattt tctttcgcaa gcgaatccaa aagcatccaa
     2341 tgaatgagag tttgtttatt cacaacaacc atagctataa atgaaaacta tatttgttaa
     2401 atgtcaaggg aaaaatgttt gagattagaa tacagaaaag ttagaatgta taactaaatc
     2461 ataatagaac gtaactttat ccaatcaaat ccctttcaca aaatattcta ttggcttttt
     2521 gtatattttt gcaaatttgc aaacttcaat aatatcaact ctcttctcac cggattcaca
     2581 ttttgaaaca tacgattgcg gctgatgtaa aatatctgcc acttccactt gagtaagttt
     2641 agcctccttt cttgcttttt taagtagcct caagaatttt tgatactcgt ctgtgtaagt
     2701 tgagtttttc actggacata ttctatatcc taatatggaa tatcccaaat tgggatattg
     2761 ttaaatatgg cttcaatcaa taattataag caattcaaca gtaattaaaa taaagattaa
     2821 ggaaaaccta tgagtaagta tttcgagatt aaaaaagaag ttggtattca aattgataaa
     2881 attagtgaac ctatgaaaat tacttttggg acaggttggc aaatagatat aaaatatcgc
     2941 tataaatgtc catattgtca gactttagac aatctacata aatcaataag agcgaataac
     3001 gatggggagc atttactaat taatacttcg gatacatgtt tcaattgtaa agcttcttat
     3061 aaaccgcaat taattttaaa gtgtgatttt tctgaattaa gaaaagaaga aaaaataaaa
     3121 agtaaatctg tagaaataca acatgatgca gaaacatctg aatctaatat aaatctagca
     3181 aatgactcaa attccaagaa acaagaagct ggattcataa aacgattaat tgtagcgtct
     3241 tttaaatgca catactacct atttgccgac agaaaaggaa atataataat gggtatcgtc
     3301 gtgatcatcg gcctcattgc aaggctattt tctgataaac atgaagaaat acctttagga
     3361 ttagcgacat tattggtaat tatagcaatt gctggatttc gaaaccataa aatatataaa
     3421 agctctgaat taaaatttca ccaacaactt ggagaagatt gtctcgcggg actcagagat
     3481 atgtttaata ttggattaaa tgaaaaggta ttttaaatag aggttaatat gagtaaatca
     3541 ttaagaaatt tttttaatag aagaagcgtt aaatacttcg ctttcgctat tataagttat
     3601 tttctattta tagtaattgg tattaaagcg atcagctata tttctccaag cgaccttaag
     3661 ttttgggaaa atattgtccc tatcgcaggg ttattttttt caggaatctt gggctttatt
     3721 cttgataaag aaaaagaaca aggattaaaa ttatatgaaa agaaaattga agcatgtcag
     3781 gattttctta aagtattaga agatgtaatc gaaggtggta taatcctact taaccgtgat
     3841 attacatcaa agaaagatga attaaataaa attttttttg cagtatccaa acttagaatg
     3901 cattttgaag aaaaagacat tctggagatt atcggtaaat gcacagacat ttccgatgca
     3961 ttaaatgaga gaaggaaaaa taataaaaat gaaaaagatt attatcagaa attgtcatca
     4021 gctctatttg gaatatctaa aattttcaaa gaactgttag aacaactcaa accagaaggg
     4081 caaaaagaga atctcaaaga accgaatcaa tttgtgatag ccatagatga gtttattcta
     4141 aatgtaatac agccaacaga ccaaagccat gtatctgaaa acacttggca tgtaaacgtt
     4201 ggagaagatt ataatattgc agacccgaaa gatcaatata gatgttggca agacatgctg
     4261 aattttggct tctggagtgc gggaggagct aagcgttatg cggatgggac taaaaaatta
     4321 aaggaaggtg ataccattta tgcatatcta agtggtaaag gatacattgc tcgaggggac
     4381 gttaaagaaa aagcaaagct agtaagagat tttttttctg gaaaagataa aaataaaatt
     4441 atttcaaaat catttttaga acatctgaat gataaatctt ttgaaggttt tcataatgca
     4501 gatatcggag aatatgcggt taaagtagaa tggaatttca aaaatccacc cgttacgaat
     4561 tctgatttgc atattagccc tatgacaatt tgcaaaatga atcaagcaaa tcttgccatc
     4621 ctagaaagag aatttcaaac caaaaaagga gagattaaaa aatgaaaaaa cttttattca
     4681 atatactttt aattttaaac ataattttta ttaactgcgt ttctgttgaa gaacgaacta
     4741 atcaattaaa tcatgctgct agatacgggc aaaaagatca agtagaccaa ttgataaaag
     4801 atggggctgt tctttcgaaa gatactttaa attccgccat aatttctggg aatatcgaag
     4861 tagtaaaatt tttgttaagc aaaggcttgg atataaatgg tagtgataga aataaaatgg
     4921 attctcttac atactcattc gaaagtccta aaagccagaa agaagaaatg tttaaattct
     4981 tgttagcaga aggtgctaaa atggaaaatc cttataatat cctgtattta ggtttacggg
     5041 atatgattac gcctgccgtt actgctgcca ttgaatcagg ggcgtatgtg gataattccc
     5101 tagtttttga ggctggaact cctgaacatt tagaaatatt gttaaaaaat ggtgccaata
     5161 taaatgctca aaattcaagt ggatatacat tattgcatat aaatattata tgttgttcgc
     5221 ctcgtcccat tagctctaaa tttcttatag agaagggcat taatgttaat ttaaaaaata
     5281 attacggaca aaccgcgctc catatcgcaa taaaagaagt agatcgagta aaattcttat
     5341 tagaacaaaa aaacattgat atcaatgcaa gagataatga caatgcaact ccattacatt
     5401 atatttggga cgctgataca gcaaaaattc ttctatcgaa gaaaggaata aataagaatc
     5461 ccattgataa gttcggcgaa actccatacc aaaaagttag aagagaagtg cttgaaagag
     5521 aaaccgatag aaataaggct ttattatata ctctaaagca ggcaggctgt cattaatcag
     5581 ctaacgccta ttcaccacaa cttgctccat atccggcaaa actaaaaact tatctttcga
     5641 tataaaacga ttcagataat ggtaattatc ccctaaacag ataagtggag tgagcgttat
     5701 tggacatgta ttccatataa agttttcgtt taaagttcgg tcaatatcaa accacaaata
     5761 gagatgataa tcttttgctt tcttatttac ttgttctata ttattcagag tcttttgata
     5821 gaatgcattt ttaatatcat acagttcatc tttgtcctct tcgagccaag ctgaataaat
     5881 ctgcctaaac tcttcactcc aatcttctaa attaaaaata gaatttactt ctttgtatat
     5941 acctttatcc gcaggtatag cttcttctgt ttgcatatcc cagataattt cttttgtctc
     6001 tggaacccac atgagtttat ataatttatt ttttaaatct tccatctttt aataacttca
     6061 aagcttttcg tgcttttctt gcttgctcac catctttact gtttttagct atactttcga
     6121 tttcttctat tgtcttatct taaaattcac tcggaaattc tgctcgaata cttccttttc
     6181 tatttttaga aataatgccg tccgccgttt catttactga atcgtttgaa acaacgatag
     6241 gaggaattaa aatttcaaga ctaggcataa aattaaaagt tactacaagt aataaagcca
     6301 aagcaagaaa aaataatttc ttttctaaat ttaaaatcct ttccataaag taaaacaagc
     6361 atattaaaac tattttagaa tagcaagaat aaatcttttt cttgctattt actctttagc
     6421 atttactctg agagtatgga agctatgaaa agacaaaaaa gaacgaaagg tgcaattgta
     6481 gagattcctt tgaataatgg atatcataca tacggaagag ttctaaaatc cggaatggct
     6541 ttctatgatt taaaaagtaa tttacgagat aagaaagcat cgtaggctaa cgttcattca
     6601 ccacaacttt ctccagatct ggcaaaacta aaaatttctc tttcgaaata aagaatttct
     6661 attgacattg actcaaatga aattctaaat agtgagggga atataaaaat gcaaactcta
     6721 gagatacaaa ttcagaatga tggtgattat caattatttt taagtttagc aaatagatta
     6781 aattgtaaag ttattaaaaa gcccgctcta aaaaagaaag aatggcaact aacgaacgaa
     6841 atgaaatctg aactagatgc gagacttttg gcacatgaaa aaaatcctga agaaggttat
     6901 tcaatggcag atgtaaaaaa agaattggag aaaagacttg ggagaaaaat acaagttaaa
     6961 gccagaggct aagaaagatt tagttcaagc cgttgaatgg tatgaaaaaa accaagaagg
     7021 agttggatta agactttatg aagaagttta tgataaaatc gaagcattgt ctaaaaaacc
     7081 aaattctcat tctacttttt atagagaatt tcgcaaagca agtttaaaag tttttccttt
     7141 tctgattata tacatcataa aagaacccat tattttaata ttcgctattt ggcataagag
     7201 ccgtgattca aaaaaattat tagaaagagt tgatagaact gagaaagata aataaatatc
     7261 actccttcta atttccattc acaaaataca caattggctt ttggcatatt tttacatttt
     7321 acatacttat catctaagtc tgtaaaatat aaaaaacaag aattactcgt tgtaactatc
     7381 gatcggtaga caagcgcaaa ccagatttct atctccgtaa acgttgtcaa cgcgacctac
     7441 aacaggccaa tacttatgtt ctttaagata tggaagcgga taggctgcca attctctaga
     7501 atataaatga tcccatttat ctgacattac catcttggca gtgtgtggag agtttttaag
     7561 aggattgtct atttcgtcta acttcttatt ttcgatatca gttatctctc tatgaatttg
     7621 aatcattgct tcacagaatc gatctagctc ttctttggat tcactttctg ttggttcaat
     7681 catcaaagtt ccagggacag gccaagacat agtaggtgca tgaaagccaa agtctatcaa
     7741 acgttttgca atatcatcta cttcaatttt tgctgttgct ttgaagcgtc taacatcgat
     7801 tatacactca tgtgcaacta gatttttatt tcctcgatag agcacaggaa agtaagactc
     7861 taatctcttc gcaatatagt ttgcatttaa aatcgccatc ttagttgcat aagttaatcc
     7921 ttcgcctcca agcattgcaa tgtagatcca agaaatcgtt gtaatactag cacttcccca
     7981 gggagcactt gtcacagaat tactttctcg tttggatcca ttttctacta acgcatgacc
     8041 cgggagaaat ggaactaaat gagaagctac cccaatcgga ccaacaccag gaccaccacc
     8101 accatgtgga atacaaaatg ttttatgtaa attcaaatgg catacatccg ccccaatttc
     8161 acctggactt gtgagtccta cttgtgcatt catattcgcc ccatccatat aaacctgtcc
     8221 gccattggta tgaatgattt cacaaatttc tttgatcgtt gcctcaaata ctccatgagt
     8281 agatgggtag gtaatcataa gagcacctag ttcattttta tacttctcag ctttttcttt
     8341 tagatcagaa acatcaatgt ttccattttc atcgcaagca gtcacaacaa ctttaaatcc
     8401 agccattaca gcggaagcag gatttgtccc atgagcagaa gttggaatga ggcaaacagt
     8461 tctatgcccc tgattcttag aaagatggta gtctcgaatt gcaagaagtc ctgcatactc
     8521 tccttgagaa ccagcatttg gttgaagaga aattgcttta aatcctgtta tctcacagag
     8581 ccatttttct aattgagaaa acatttctct ataacctaac gtttgatcta tcggcgcaaa
     8641 aggatgcagt ttagaaactt ctggccatgt aagaggtagc atttctgtag atgcatttaa
     8701 cttcatcgtg caagatccaa gcggaatcat ggaatgagtt aggcttacat ctcttgattc
     8761 taattttcga atgtatctta gaagttttgt ttctgtgtga taggtattaa agaccggatg
     8821 agtgagatac tcgctttttc ttaccaactt ttcagagaaa ttataagcaa ctgtatccgc
     8881 tgcaatatga acgttaaccg cattgccttg attaaaaata gaaagtaaaa caagaatatc
     8941 ttcttttcca actgtttcat caatagaaat gccaatccaa ttttcttcat aacgcaagtt
     9001 tacattttta gactcagcaa tttttctaat ttcagattgc tttgttttat ctactgcaat
     9061 cgaaagtgta tcaaaaaatt cttttgaatt taatttaaat ccaagtgatt cgattccctt
     9121 tgcaagtatt gaagtaaaaa gatgaatccg agttgcgatg tgtctgattc cttctggtcc
     9181 atgataaaca gcatacatag aagatagaac ggcgagtaaa acttgagccg tgcaaatatt
     9241 actagttgcc ttgtctcttc gaatgtgttg ttctcttgtc tgaagcgata gacgcattgc
     9301 cggagcacct tgagaatctt tagaaacgcc aatgagacga cccggaagac ttcgcttgta
     9361 ttcttcttta gtagcaaggt atcctgcatg aggtcctcca aatccaagag gaagtccaaa
     9421 tctctgggtg cttcccactg caacgtcagc acctaattct ccgggagatt ttaaaatagc
     9481 aagactcatt aaatccgctg ctgttactac taatacacca tttgcatgcg cttgcttgat
     9541 caattcctca taatcataga cattcccatt ggttgctggg tattggatta atacagcaaa
     9601 gaaagattta tctgctttga actttttata atcaccaacc actacattaa acccaactgg
     9661 tatagccctc gttcgcacaa cgtctattgt ttgaggatgg cattcatcgg aaataaaaat
     9721 tgagtctcca ttttcatcgg acttaaccgc atgacaaaga gtaattgcct ctgccgccgc
     9781 tgtggattca tctaataaag aagcatttgc aatctcaaga cctgtcatat ccataatcat
     9841 ggtttgaaaa ttaagtaagg cttctagtct accctgagcg atttctgctt gataaggagt
     9901 ataggctgta taccaacctg gattttctaa aatatttctt tgaatcacag caggcacaat
     9961 acaatcatta aatccagtcc cgatataatt cttaaaaatt ttatttttgg aaaaaattct
    10021 ttttaattct tgtaatactt catactcact cttagcttct gttacttgaa ttggattttt
    10081 taaacgaatg tttgcaggca cagctgtatt tatcatagcg tctaacgact catatccaag
    10141 agttgcaagc atctctttta cttctttaga gtcagaccca acatgtcttt ttaaaaacgt
    10201 atccgcagga gttagagggt tagaggattt ttcggaagca accgaactag aaatcattgt
    10261 cattttcatt tccttttgag cctagacaac gagaaggttt gggcgattcg aaatcacttt
    10321 agtgttcaaa aggatttcga ccgcgctttc aggcgtgcat ggcacgctct tcaatcgcta
    10381 tcgcgtaggt ctaagcgaat tactttccat aaattcataa aaaggcatgc tgtcaaatac
    10441 gattatatca ctaaaagcat catagaaatt catctagtaa cagctttcag tttaagttta
    10501 aatttcataa gttctaaagt atgattttga aataacaaaa atcttctatt taaattactc
    10561 actcaccttc cattccctcc ctaatataaa aagcaatgac tgagcgttaa aactaggttt
    10621 gcttctattg tcattataaa atgacaatag gtttgagcag agtaaaagag aggaagcaca
    10681 acgcttttcc actcatcgtt tgcttctaat tttataaatt ttgtattagg agagttattg
    10741 aattaaatta aaataccaat actttcagaa aatttcttta caaatatata tcgtaaatat
    10801 acgattagtt atggcacaaa ataagaaatc tgaatttaaa gaaaaaattc aaaatctttc
    10861 tgaattagca aaatcaattt ctcacccggc gcggatatcc atactcaaaa caattgcaca
    10921 aaaacaagaa tgcatctgtg gagaaatagt agaagttttg ccattggcac agtcaactgt
    10981 atctcagcac ttaaaagaat taaaagaaat cggtcttatc aaaggagaag tagaaggagt
    11041 caaatcctgt tattgcatca attggaagaa agtagaagaa ttcgaaaaag agataaataa
    11101 actattccaa gagttatata cattcaaaaa caaggattat tgctgttaga tgttcatttc
    11161 aataaattca gaaataagtc aagtttaaaa aggctttcgc atatgttact tatgaatcgg
    11221 cttctaaatt atagcaagtt taacattaaa aataccagga gagaatatga agaaaattct
    11281 agtattgtgc acaggaaaca gttgtagatc tcaaatggca gaaggttggc ttagacactt
    11341 tgctaataat aaagcagtaa tctatagcgc aggtatcgag acacatggag tcaatccaaa
    11401 agcaattcaa tttatgaagg aagttggaat cgatatatca aatcacactt cgaatcatat
    11461 caatgaatac aaagatattg attttgattt tatcttaaca gtttgtgacc acgcaaaaga
    11521 aaactgtcct tatttcccaa ctaacgcatt gagactgcat cacaactttg ttgatccatc
    11581 aaaagtaata gggacagagg aagaaattgc aaatgctttt cgtatgacgc gtgatgaaat
    11641 acgtgattat tgtaaaaatt ttattaagga aattgaatga gcttttttga aagattctta
    11701 acaatttggg taatactttg tattatcttt ggagtattac taggtcaaat agctggagat
    11761 tgggtaagta aactaacaaa tatgagtttg tttgaagtaa atattccgat tgctgtttta
    11821 gtatggttaa tgatttatcc catgatggtt cagattgatt tctcatcgat caaggatgtc
    11881 ggaaaaaatc caaaaggact tattcttaca ctggttgtga attggcttat caaacctttc
    11941 acaatggctt tatttggttg gatctttttt ttaaatttat tttccacctt ccttccagct
    12001 gacagagcaa atgagtattt ggcaggagcg attttacttg gtgcagcacc ttgcactgca
    12061 atggtttttg tctggtctta tctaacaaag ggtgatcctc tttacactct cgtgcaagtc
    12121 tcaatcaatg atttggtaat tctatttgcc tttattccga tagtaggatt cttacttggc
    12181 atttcaaata tttcaattcc ttataataca ttaatttatt cagtagttat ctttgttgta
    12241 atacctttgt ccgctggtta cgtctccaga aaatatatca taaatgctta tggcaaagac
    12301 tggttcgaaa ctaaatttct gaatacttta aaacctgttt ctattttagc acttcttgct
    12361 actttggttc tactcttcgc atttcaagga agtattatat taaaaaatcc ttttgatatt
    12421 ttgctaatcg ctattccact aacgattcaa acatatttta tattcggaat ttcttgggct
    12481 atgggtaaaa aattgaattt gccacataat gtttgtgcgc cagcatcaat gattggagct
    12541 agtaattttt ttgaactctc tgtagctgtg gcaatcgctc tttttggagt aaattctgga
    12601 gcagctcttg cgactgtcgt aggtgttctg atcgaagtgc ccgtgatgct aacactcgtt
    12661 aagatggcaa atcgatggag attctaatct ctttttaaat tatatatctt tactttgcca
    12721 ttgtattgaa taatatttcg gatcttttta atttgaatca tctcatctgt atctagattc
    12781 ctaaaataag aaatacaacc tcgaattaaa gtgaaaggtc catatacttc agagggagct
    12841 gtgcattcca ccttgctaaa ttcaagattc tttccattta aatctctaat tagctctctc
    12901 caagcgattc ttgtttcgag actcatatgg tcgtaagcta aatcaggagt cattccagga
    12961 tctgatgtaa atctttctcc cacatgcttc caaaaaaatt cattatgctc tttacgattt
    13021 aaaataattt tgtccatcga tttggaatca tttctagtcg cataaaaaac agcttcttta
    13081 aagagttcct cttttgttgt cttcaaattt tccaagttat agtttgtatt taagcaacta
    13141 aaccaaacta gattcataag caagtatagt tttttcataa aattattttg atattaagaa
    13201 atttcttcag atttagaaag atgcggcagt tccttaaatg gatcattaga aactaccgca
    13261 agaattcaat ttttaatttt gagaaaaact agtccagcct ttccaccatg tatcgtttgc
    13321 atctctcaca gcaccatagt atgcagcact tctaaaacca gaagcgccag aagtatttgt
    13381 gaaattcgtt acagtaattg tatctgttgc agatatcgtt ggtgtcgatg tagccaatgg
    13441 tgtaaaatca ggacttgtct ctgatattgt tgtaactact tgcgtagata ttccggctga
    13501 cggagtagca agattcgtta ccggagtagg acaatcactt gtcgcagatc ttgtataaaa
    13561 aagatttgta atccttgcat caactgttct agaatcagtt ctacaaccaa cagctttatt
    13621 gtttccataa ttatctacaa gtatatttgt ataatctccc aattgaaagg tcggagtcgc
    13681 tcttcttaca aagatcgcat ctccatgatt tgttacagga ccaccaagtg ctccatttcc
    13741 gcctgtagaa ccgccagctc caacacaagt cacatttgca aagtatggat cagagcaatc
    13801 tcctccgttt cttgcgcttg acgtgcagga caaagcggag tttccatctc cctctataca
    13861 tctagtaata tcatcatttg ttggcgctgt agaggcataa atttgcccaa tcgcaaattg
    13921 gtaagttcca ataaaaccat cagcaaaatc aaactgatca tccctatttc cggttgaaat
    13981 aagataacga gcattaaacg ctcctcccca ttgttcaaac ccatcatcaa atccacgatg
    14041 acactgaaca taattaaggg aagttcctga tccaacagcc atcaaggaaa tacaatttct
    14101 ctcatttcct ggagaaaacg gagcgccagc aaattctact ctcagataat tgattgatcc
    14161 agaggaatct gagttattgc ttccaccata attaccgacg tctccttcgc ctatcgcact
    14221 actaccagct cctccaaatg tttgaactcc atttccttga ataataattc cctgccaatc
    14281 tgcgggagct ctcgatcctg ctgatttttc agatgtaaat accaccggtg cattcgctgt
    14341 tccattcaca aaaatctttc cacctggttt gattaataaa gccccaccag gagatgcaga
    14401 accaataacc gtaactcctg gtcctattgt gattgttcta cctgaaggaa cgattactgt
    14461 tccattaaga tagtattgtc catctgttct cgtccaaaca acgtcagacg ttatagttcc
    14521 agaaagaatt cctatgtaat tcctaggaac agcaatttca atggaaatat tggttggaat
    14581 aaagtcataa ctcccactcg gaacactaga aggacttgac gttccatcta cttgcgatgc
    14641 agcaatatta ctccaactac tctctgtgct agtaatatcc ttaccagtgc ttgattctaa
    14701 aagtgctctt gaaatagctt ctgtaacagg ttgtaaaacg ttagacactc cattattatt
    14761 aatcttaagt cttcttgtct ttaaaactct tccagcagat gttgtcactt ttatatcata
    14821 tgtatttcca acatttggaa cagtaaattt aataccatta aaagaacctg ttgcgtccgt
    14881 tgtaaaggaa gcaaccactg tatttctagg gactactgta tttgaaatac caacagatgt
    14941 tgcagcactc gaaagagtag aagtatcaca atataccctc acattttgac caaaccctgt
    15001 aaatgaatca ggattcgtgc taagtggcgc agtagttccg aaactattca cagcatctac
    15061 taccgttcta ccacaagttt gtaaaacata ctgtgctcct ttagatagat ttccggaaga
    15121 aagattagca gcagacaatt cactatgagg tgcaataaaa gtagcaggac atcctgtcgc
    15181 attaccgttg ttagtcgcag ttctgtcaaa gttagtgcca gggcaagcac ttccatcaat
    15241 cgcagttgca gaagctccct gtgaattatc aaaataataa ccatttggaa tagaatttgt
    15301 ggagccaaaa aaattacaag caccgactcc attgctacct accagaccac ctgcacctgt
    15361 gggtgcacta caattaggcg cactgtttga aacagtgcta tttactgctc cagttgtatt
    15421 gttaaatcct aagatgcaac cacctggagt tccattaaag ctaacacttg ttagttgtcc
    15481 ttgtgtagta taagctgctg ctgttgcgga tgacggacaa ctagtagctg tcgaacccca
    15541 acccacctta tcactgagaa cagcagacaa agtagtaaag ccatttggat ttgttgaggg
    15601 tggaggcaat gcaggtcttc caatcgttct aacactcgct ggaggtaaaa tggaaaccga
    15661 gctttctcct cttcctaaaa tctgaactgt tgcatttggt attgcctttc ctgtgctatc
    15721 gagtattgtt cctgaaatgt ataaaccagg gtctctaggc tttggaggat aagagaccat
    15781 taaccgactt aatgcacttg gcttttcttt tgattgtctt atgtattcac atgaaaattg
    15841 aaatacacta atagacatta atattgatat aaataatttc tttttcataa aagtatttct
    15901 cctgaatcta tttaaaagtt gtaagatgct gagaaggtaa ttgtaggtcc aagtctgtaa
    15961 gaattgatag ttgtatcttt tcctctccaa ggatcttcct gcacgatttt aattctagta
    16021 tctgttaaat ttctagcagc caatttaaaa tcccatttat ctttccattt caaattataa
    16081 accatgtcaa gcattccaac aggtctttca taagtatctg gaattcctat accgcctacc
    16141 gcattaattc tttttccaaa ctcattgtat aaaacggtaa ttgtatgatc tcctacttta
    16201 tctaatttat atctaacgtt tacgttatag acataaggag attgtccttg tagtggacga
    16261 gataagttag taggtctttt aggatcgtta ggacttagtt gataatacaa ccaatcgtta
    16321 aatgttactt ctgatttaat taagaacata ttaaatccaa cagaccattt ttctagcttt
    16381 tctgaaataa aatcgagccc ttttcttgct tcgaactccg caccttgaat atatgcagat
    16441 ttagcattgg tataggtata tctaaattga gaatctactt ccattacctt ttctattgga
    16501 gaacttaaat tttttctaaa tacaccgaat gaaattagat catctccctt tggataaaac
    16561 tcataacgta aatcatagtt atgaatgtaa gatctcttta gatatggatt tcctttttct
    16621 ggcggtccac ctaggatatt tgtaaactca aacgaagaca tctctctaaa atctggacga
    16681 gtgatcgttt ctgtataagc gagtcgaata tttgttctgt cgtcgagcga ataaacaaaa
    16741 ttagccgaag gaagtgtatt cgtattttta ttgatagctc ttggctgtct gtaagttgga
    16801 tcaattaaat tttgctgaaa ctcatttgta atcgcataag ttttaaaatc ataattagca
    16861 gcaaatgaag caagtctatc aaacggattg acagttgaga caacttgata gttattttca
    16921 ttacgcaccc caccgataaa tctaagcttc ggtgtaagcg gcatatctac ttgcgcatat
    16981 ttagatgtta atttttgtct tgcttcataa gtatctgtag gtcttgttga ttcactcaca
    17041 taatagcctt gaacacctct gttaaacgga tttaaaactg cctcaggtgg aacagggtaa
    17101 acggcaggcg taagagctcc tccaccaagg tttgttcctg gatccgcttt tctaaaataa
    17161 tatctctgtg catcaaaacc tctctctctg gttaagtaac tatatccaac tttgaatttg
    17221 gattgaagac caccccactg agtaaaaggt atttcataat caaaactaat atatctattg
    17281 atatccaaag ttctagagaa gaagcgactc ttggaaagct gtgactctgc aagaccagca
    17341 cctgcattgt attgtggaat ttgtgagttt gttgcagggt cagtcgttag tggaacttga
    17401 taatagctat ctctcatatc aggttgattt ctgaaagctt ctgattggga aactttccaa
    17461 tccaatttcg taaatttatc tgcgatctta ataatatgat ctccacctaa ctgagtatta
    17521 tagagattac gcataatata gtttagcttg gttgcatcaa tttgctgccc acttgcaaaa
    17581 ctagatcctc cataattcca aacttcctta tcattattca cactaaagaa attcttccag
    17641 tgaactcttt gcccatttgc aatttcgtaa gtagtgttaa atgcagagcc ccagttaaca
    17701 gattcattcc atattttttg attataatca taatatttaa ttaggtatgc attttttcct
    17761 ccaactggag ctctaggaac aataccacct actctatttg ttacatctct ttcttctcta
    17821 aattgtaaat catttccata cgttagtgca aatataactc caagttttcg gtttgctacg
    17881 ttgaaaacat ttccagtcga aatattaaag cccttattag gagatgctac agttgtattg
    17941 gaagaccact gattattaaa agcaagagat cctgcagtaa ttgcaccaga gttgtatcca
    18001 aaagaacctg gaccaactgc attaaactga acgcctggga gcgttgaaac gatgtcaggt
    18061 ctttctcggt taccagctgt tagccctaac cagtcttgtc ctccaccaga atatgtttga
    18121 aattgtttcc ctgtggtatt attattataa cccattgaaa atccaacttt caaaaagaat
    18181 ttgtctggat aatctttggt ttcaatctta accgttccac ccgcaaattc agcagaatct
    18241 tctggtataa atgtttttga aacgataatg tttttaatca atgcagaagg aaataaatcg
    18301 agtggaacaa cccttttatt tggctccggt gatggaagtg gaactccatt taaaagtgta
    18361 tttgaatatc tttctccaag accccgaaca aaaacatatt taccaccaac aagggtaata
    18421 ccggttatcc tccgtgctgc atctcctgcg cttgaatctg gtgtcttggc gatcgcctgt
    18481 gcagagatcc catcagaaac agtggaagct ttcttttgta atttcaataa ggaagactca
    18541 gtatcattca tagcccgacc ttccactacg acggtatcta attttttaat tcccattgcc
    18601 acattgattt ttgctggttt accaccgcta acagaaatct ttcgtttctg agtatccatt
    18661 ccttgcatga taaattcaat atcatactca ccatcaggaa cacccgtaat ggtaaatttt
    18721 ccatctaaat ctgttcttgc tgatttcgaa atagagcgaa tcacagctgt taccccaaac
    18781 ataggctccg ctgtctgcgc atctaaaaca tccccctgaa ttgatcctgc tcctgttttt
    18841 gcttgtccaa atactgccga agatacaagc attaaaagca gtatcagaaa gttaatttgc
    18901 tttttcataa tttaatctct tattattcct ttcagtcctg cttataagaa tacaaggatt
    18961 agtgttacat ttttattact tgaattttaa ttgtagatta cttacatttt aagaaatgat
    19021 tacaaggaat cacttgactg aaaaatgaaa ttcattacta tttaatgaat cgttttgcaa
    19081 attatgaatt ttgattgctt atgaaaagta aagtttcata cttaaaccaa attgtcattt
    19141 atgtgcaaaa ccaaatatta gaatactagt aaaaaccaag tttaaccacg ttagtttaat
    19201 tgcgaagcaa aggaatgctg tgataaaaaa taaaattcaa aaaatatatg cagctaaaag
    19261 tagcctaaga gcaaaattat tcttaggatt cattatactt agcttggttt tatcatcagt
    19321 tcttggtttc actctttatc aagtgtctag ctcggtttat tataagtcat tcttacaaca
    19381 taaaaaatct cttggtatat caatttcgag ttcgattgat ggcgagattt ttcagtcctt
    19441 tataactgaa aaatcttctg agactcccga gtttaagcga tatttcaatt tcatgcaaag
    19501 ggtttcggca aacgaagaat atatcacttg gatctattca gttctcattg acaaaaaaac
    19561 aaataagtta atatatgcga tagacgctac ccacattagt gaagacacta tttggataga
    19621 gaatatgttt ataggactta aagtctatat aaatagaaat ggaaggctag ttgtatattg
    19681 ggatagtatt gaacatgtcg aagattttca aatcgtgaat aaaggaaaga aattcgaaat
    19741 tgaaattgat tccttaaacg aaacagtaag tattaataaa attcaaattc taaaagtaac
    19801 taagaaaaat ccatttactg tagaaacaaa aaaaggaatt ctaaataaag aaaatcctgc
    19861 ccaaacagcc acaatcaaag ttgaagaaga aagcctagat gtatctctta attttgcaga
    19921 aaagaatagt caatcctcaa ttccaggctg ggaatttatt gaaacagagg atttagtaaa
    19981 taaaatcaaa ggttctatta atacctgcac ctttcatatg caaaatgaac cagagcaatt
    20041 ggcttatgga acttttaata tagtcatcgc tcctatattt aaaagtcata cagagtgtat
    20101 cggagctgta attttcagtg tttccatttc tgatatccgt caatttaata aaactatgat
    20161 gatcgccgga tttactgtga ctataatagc gcttatttta tcagtgatag tatcctatat
    20221 tttatccatt ttttttacag accctctgca taaattatcc caagctgttg atgaattatc
    20281 atcaggaaat atggaaacta acgtaataat acaatcaaaa gatgagtttg gtttcctgtc
    20341 agaaaaattc aatgaaatgg tagcaaatct aaaaaaggcg tataaagaaa aagaatcgtt
    20401 agccgcacta agacatgaat taaatgtagc acaaaaaatt caaacggcaa ttttaccaaa
    20461 atcaattcca aacttaccaa atttagatat agccgttaat tactacccaa tgacacaaat
    20521 tggcggtgat tattatgact ttcatatgat cgataaaaat aaaattggaa tttttattgc
    20581 tgatgtttct gggcatggaa ttcctgctgc aatcattgca tctatgctaa aaattgcctt
    20641 ttcaattgaa tctgcttttg cagaagatcc atcccgattg ctttccagaa tcaaccaaag
    20701 tatgctaaat aaagttggaa caaactttat tagtgcaagc tatatctata tcgatctaga
    20761 gacaaacaag atcaaacatg caaaagctgg tcatccatct atctacattc attctcctga
    20821 gacagacgaa gtaaaagaat ttaaccaaaa aggaaagatt attggtttat tcccagaaat
    20881 tcaaactgag actgttgaaa tttctatcca aaagggagac cgcatcattt tgtatacaga
    20941 tggtatcatt gaagcaagaa attccaaaga agaaatgttt ggagaagaat tatttgttgg
    21001 ttatattcga aaacacaact ctatgaagcc tgctgacttt gcacgattgg ttatgaatga
    21061 tgtaaaaagt tggataggaa acaacgcatc aaagcacagt gatgatgtta ctttgatggt
    21121 agtcgatatc aaataaaccc cgaagatcgg ggcttatttg aatgcaccac aattaattct
    21181 taggtggtat gaaacaattt gctattttct tagcttttag tttttctcta agagctactg
    21241 catcttctct ttttgcaaaa tccccaattt gaacaacata tgttccattt cttgagaata
    21301 agaaaatttt ctcagaaaaa tcttttttta tctgatctct aaatgcttct gctcttctct
    21361 gctctttaaa tactccaatt tgaactgttt ctccttttgg ctgagatgcg ttatttgtat
    21421 tggaagttgt aggagtagga acattcacag ttggaatttc tgctggcgca gttgtcttac
    21481 tattattagc aggagctgtc tttacaggtg cgggagctgg agccggtgca ggtttcttat
    21541 caggagcgac aggcgtaaca gtctgcggtt tttttgcatc aggtttcttc gctggtggag
    21601 cagtttcttc atcgatgtca tcatcatcgt catcgtcatc atcgtcatcc acactaaaag
    21661 aatcatcctt tacaagaatt ggattttctt ccattggctt aataatatta atccctactt
    21721 ttgcaacaga ctcttctttg aagttcaata tctcagctgc tttttctgta acttcaataa
    21781 tgcttccttc atcagccggt ccgcgatcat tgatagtaac atctgcttct ttattgtttt
    21841 ctaaattctg cacttttaca actgatccaa atggaatcgt tctatgtgca gcagtcattt
    21901 taaaacgatc gaagacttct ccacttgcag ttactttacc ttgaaattga attccatacc
    21961 aagaagcaaa gccaatttca tcatatttag gagttgcaat cgtctttgaa gtctccttag
    22021 tttcctcttt aattacttgg ctttcttcga ctgttttagt agcctctcta atttcttgag
    22081 tagattggca agagatcatg gctacgattc ctaaaataat cagaatttgt ttcatgtgtt
    22141 tagaacctac ctattagttc attaatctat tacttgaaat actaattagc tttatcaggt
    22201 gtaaactctg agaaaatcta cacgatttag cataaatact tcacgcttat tattaataat
    22261 cgacagtaaa ttaagagaga cttggcattt ttctcaatta atttgaattt aaaaattgaa
    22321 atcctttaga tcgagggatg gttctacaga actcgcttaa tttcaattcc actcatctag
    22381 catttttaat agaattttaa ttttcttgcc taatttattt taagttcata gtttgatatt
    22441 caaaagggtt tctaatttaa gagtaatagt atgaaaaatc gatctgatac aatgactcca
    22501 tctaactttt ctgggtatgt gaatacaatc aaagaaaatg taaagagaaa gtattcccat
    22561 tataaagtaa acaaagtgcg tcaacattat agagatgggc tcagctttga agacaatgca
    22621 gaatatagta aggcaattga atcttacctt gaatgcctca aattagatcc tgattatata
    22681 aaatgcttaa agcgccttgc taaactctat tccaatctag aagagcattc gaatgcaatc
    22741 cagacttata caaaaatcgc aaagctacaa ccaaatgaag aaaatttctt tgaactagga
    22801 caagaatatt acaaacaaaa tcgttttaaa aaagcgattc tttgcctaaa aaaatctctc
    22861 tattataaac gaagatttat ccaatcccat agacttcttg caagtattta tgcacgagta
    22921 gaaaacagag acaaaacaga acagtattta tctaatgtta ttaaaatcga tccaaatcat
    22981 aaaggatcca tggaagaatt aatcaaatta tatttcatac aaggaagata tagagactca
    23041 ctaaacttat tagaaaaata taaagaggtc tatgtagaag attcgaatat aaaattttta
    23101 aaatcagata tctatgtgaa gatgggtaaa tattcaaaag cattaaatat tgcctatgaa
    23161 tctcttattg aagaagaagg atttcagtct ttcattgaag acatgcaata taaaaaagtg
    23221 aatccaactt taaaagaaaa agattttcta cgaaagatag ttttaattaa aaacgagaaa
    23281 aagaaattgc tagaagaaaa tttaaaaagc tatcaatcgg gcgatgaaac aaaatttcca
    23341 gatccaaaaa tctcctacga tcttgcttta ttatatttac ttctaggaaa tcaaaaaaaa
    23401 gcgatgaagt atttattctt tgcaagacaa ctcaatgaag aacgaaaata tatataggaa
    23461 catttttatt taatgcatat aacgtttcta ttcctaataa tttttgtttt aaataattgc
    23521 ttctctatat ttcgtccaac gcctgtatta gaagatgata aatcagatat tctcaaagcc
    23581 gataaacagt ttattctatt ggacgataat ctaaaaccaa tcgaaaagcc agattttact
    23641 ggtttggata aaaaagtcaa agcattacaa tctgcaaaaa catttcatga tttaaataat
    23701 ctagctgttt atggagtaaa actttcttac atcgaatcga gcgaggagat tttacgaaag
    23761 cttcaaaaag aaaaaccaac agaacaagtc ccttttttga acttactcag aatctactat
    23821 ttactagaag aatatgatat cgcaaagaaa atgttaaatg attattataa ggcaaatata
    23881 caggacaaaa agaaagtctt tgattttatg aaatatctaa aatccacaaa taggatggag
    23941 gagcatgttc tgtttttaga tgttatttct agctaccaag aacatgagat gcaagcttta
    24001 gaagaattag gaaattattt tttatccatt ggtgagctag agctcgcaaa atcttatttc
    24061 gaaaaaatac ttactatcta tgcatacaac ccaattgctc ttcaatccat gatgcaaatt
    24121 cattacacaa atgaagcatg gacagacgtt attacatatg ccgcaccact acgaaaacaa
    24181 aaaacaaaag aaaaaaacta ccatactatg cttgcaaaag cctattttga attaggagac
    24241 tatgcacaat cccttaaaat ttcagaagaa gcacccgaac aagaaaaaag ttcaattgat
    24301 tttttagttg tttggagaga ttctatattg tgtaatgatt taaaagcacc acttaccaaa
    24361 ttaaataaat actttcaaat tgctcaaaaa aagaatccaa ctttaaaaga atcagagttt
    24421 tttttagctt cttctaaaga aggacgacaa atgatttcaa atataattca tggatactaa
    24481 cgtatgaaag aaaaaattct atttattcta ttctgtttac taattgcttc atgtggacca
    24541 aatgagcgaa tcaaagcact agtcagaaat gcaaatgaaa tttttggtgt aataccagat
    24601 aagatgcctg gtagcgaaaa tgacacattg gcaatcatca atttaggaag gaaactttat
    24661 tttgacaatc gactttcagt caatgataac caatcctgta atacttgtca taacgtctta
    24721 aacaaaagag ctggtgttga taatttaaat ttttcacccg gggtttttgg taaaaatggg
    24781 aacagaaata ctcctactgt tttaaatgca ggatttcaaa ttgcccagtt ctgggatggg
    24841 agggcaaaaa aattagaaga ccaaaccaga attcctcttt ttaatcctct ccaaatggca
    24901 attcctgatg aaagatatct agttgataaa attgcaagta taccagagta taaagatttg
    24961 ttcgaaaaag catatggaga caaatcgggc aaaattacat ttgatcaaat tacaaatagt
    25021 atttctgcat ttcaaagaac atttaaaaca aatgatcgat ttgatgattt tcaaagaggg
    25081 aaatataaag cgttaaatcc agaggaaata agaggattag aaatttttct atctgttggt
    25141 tgtgccaatt gtcataaagg tcctctgcta ggaggaaatt cttttcaaaa attgggagtc
    25201 aaaaaacaat acgagaacaa agaagattta ggaagaacaa aagtaactgg aaaagaagaa
    25261 gatttatttg tatttaaagt tccaatgctt agaaacgttg cgttaactgc tccttatttt
    25321 cacgatggaa gtataaatac gttagatctt gctgttaaaa aaatggcaga tatacaactt
    25381 ggaaaaagtt tatcagagca agaaattgag tatatagttg cattcctaaa tgcgttaaca
    25441 gataaatcaa gggagaattg atttggaagt aatcaaaaga aataacatta gtataaaagg
    25501 cgaagggaaa aaggtaatgc tctttgctca tggatatggt tgtgaccaga atatgtggag
    25561 gttcataaca cctgaattcg aaaaagatta tcaggtaatt ttatttgatc atataggttc
    25621 agggaaatcc gatctaggct attataacaa aaataaatac gcaaaactgc atgggtatgc
    25681 aaatgatatt ttagatattt gtgatgcttt gaatttaaaa aatgttatat tggttgggca
    25741 ttctgtaagc acaatgattg ctcttcttgc tgcaaataaa aacaaaagtt tattttctca
    25801 cataataatg atttgtcctt ccccttgtta tataaatgaa gaaaattata ttggtggctt
    25861 ttccaaagag gatattttgg aaatgctaga atcacttgaa agcaattacc ttggttggtc
    25921 gagttctatc acacctgtca taatgggaaa tccagaacgt ccagaattaa cggaagaatt
    25981 aaaaaatagt ttttgcagac aagatccaga aatagcaaag cattttgcac gggctacctt
    26041 tttgggggac tatcgtaaag atttagaaga atgtatgacg cctacgctta tcattcagtg
    26101 ttcgaatgat gcaattgctc ctaaagaagt cggtgattat acaaacaaac atatacacaa
    26161 aagtgagatg gtaacaattg aagcaacagg acattgtccg cacctaagtc atccaactga
    26221 aacaatccaa gctatcaaaa attatttagc agtaaatcta acagatgaat gattgaaaat
    26281 gacgcattaa aaattgaaaa ttgtctgaat gatgctccat gcggttactt acgattagac
    26341 tctaatggaa gaattttctt cgcaaataat actctactca aatggttaaa ttttgaatta
    26401 gataatttaa ctaataaagt atggactttc ttattatcag gtgggagtag aatctatgaa
    26461 caaacacatt ttactccgct ccttagaatg caaggttatg ttgaggaaat caatctagac
    26521 cttatagatt caaagggaga taagattcct tttttaatca atgcaaagat cttagtagag
    26581 gactcaactg ttgatatatt tctttttcgt tttaatcaaa gaaaatcata cgaaaaagaa
    26641 atcttaatcg caaaaaaaaa tgcagagtta tcagccaaag ttaaatctga attcttgtca
    26701 aacatgagcc atgaaattag aactccactc aatggaatca tcggttttag tgaattatta
    26761 ttaagctcaa agctagactc atcacaggaa gaatacatac gaattataaa tcaagctgga
    26821 aactctttat tagagctaat taatgatata ttagatttat caaaactaga agcagaaaaa
    26881 gttgaacttc acatagaaaa aacaaatgtc tatcaattat tagaacactg tattgattta
    26941 attaagttta aagcaaatga aaaagaaatc gagttacttt tatatatatc gcctaatgtt
    27001 cctgaatata tttttacaga tcccatacga ttacggcaag ttatcataaa tcttttagga
    27061 aatgcagtta agtttactga aaagggagaa atagagttaa gggtagaagg taatattacg
    27121 aatctaaatt caaatcaaat agaaatcagt tttacaatta gggatactgg gattggaata
    27181 tcgaatgaaa aacagagagt tatctttgat gccttctcac aagcagattc tagcatttca
    27241 caaaaatatg gaggaactgg tttaggatta acaatatcaa ataaattgct aaaattattc
    27301 aattccaaac tgaatgtgaa ctctcaaccc ggaaagggaa gcgaatttta ttttactgtc
    27361 tcctctcatt ttgaaataaa tcccaattca gagaaggatt tatacttgga ttccttttct
    27421 gctttaatat tggatcagaa tgatagaagt cgaaccatca tttctaaaat gctagatgat
    27481 ctgaggataa aaaataaatc ttcagctaac atcgaattag ctttgagctc actcgatagc
    27541 aatgttttca atttattgat aataaataat aatttaacaa gctttagcga gtttttaaat
    27601 aaatccaata attttaacgt gtataatgac agtaaattta ccactatact tttattagaa
    27661 tcaacagtag ctgactggaa tgaggatttt caatatccct caaatatagt aaaaatcttt
    27721 aaaccattaa gaaaaaagga tttagtagat gtcttaataa atagatttcc aaacatagta
    27781 agaaagagtc taataaaaca acaggaaacc ctatattcaa tggtgaatga aaatttaaaa
    27841 ataatgattg tagatgatga tgaagtaaat cttttcttag ccaaaacagt attagatctt
    27901 gttatgccaa aagcacatat tgttcaattt aacaatgggt tggaagcttt aaagaacatt
    27961 gaaaaagaaa acccaaatat cattttcctt gatcttcaaa tgccagaatt aaatggaata
    28021 gagactacaa tcgaaataag aaaaatcaat tcttttaaag aagttcccat tatcgctttt
    28081 tcagccggaa tgattcctga agatctcgaa aaatgtaaag atgctggaat gaatgactac
    28141 ctaacaaagc cagtcaaaaa agaagacttt caaagagttt tagaaaagtg gttatctaag
    28201 taaaatcgct aattcactct ttcataaatc agctaaaaat cccaccatca cacaacacta
    28261 acacagctac tgaatcagca gacaaccaaa acccatagcg accatattta gattgaaaga
    28321 gtctttttag aattttattt acaataactt attactgttt tattatagaa agagtatgct
    28381 ttatagaata attctctctc tttttattat ttgtttgcca ttaaccgctc aagaaaaagt
    28441 ggaagttgct gtgtttgcag gtggatgttt ttggtgcatg gaaagtccat ttgaaaaaat
    28501 aaatggagta aaagaggtga taagcggtta cacaggtgga aaaaaggaaa atccaactta
    28561 tgaagaagtt agctctgggc tgactggaca tttagaagct gtgcaagttt actatgatcc
    28621 taaaaaaatc tcatatattc aattattaga aatcttttgg agacaaatag atccaacaga
    28681 tcccgatgga caatttgtag atagaggcaa gcaatataga acagctattt tttataaaac
    28741 taatgaagaa aaaactcagg cagaacgatc taaaaagaac ttagataaat caggaaagtt
    28801 tagttctcct atcgttacag aaattctccc ccttataaaa ttttacccag cggaagaata
    28861 tcatcaggat ttctataaaa aaaatcctgt gagatataac tactatcgtt acggatcagg
    28921 tagagatcag ttcctagaaa aaatttggga caaaaaataa ttatttcgat aat
//
LOCUS       DAOQOS010000068        28828 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_734_length_28828_cov_5.789907, whole genome shotgun sequence.
ACCESSION   DAOQOS010000068 DAOQOS010000000
VERSION     DAOQOS010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 28828)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28828)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28828
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_734_length_28828_cov_5.789907"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..329)
                     /locus_tag="PLF66_15085"
     CDS             complement(<1..329)
                     /locus_tag="PLF66_15085"
                     /inference="COORDINATES: protein motif:HMM:NF024144.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadherin-like beta sandwich domain-containing
                     protein"
                     /protein_id="HMZ65420.1"
                     /translation="MIWFIRKLHFLLIIMFLAFILNCSHDDSFKIKLVLPLLNSNSSN
                     TATTSGSLTNLVPSTGTLSPSFSSTVFNYTMSVGNSVSSIQFTPTSSDTTARITINGA
                     TVSSGASS"
     gene            complement(696..2810)
                     /gene="ppk1"
                     /locus_tag="PLF66_15090"
     CDS             complement(696..2810)
                     /gene="ppk1"
                     /locus_tag="PLF66_15090"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF003921.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HMZ65421.1"
                     /translation="MIEKNELQFDWSKLDPNNLDHPELHISRELSWFEFNQRVLDQAY
                     NESHPLLERVKFLSIVSSNLDEFFMVRLSTILKKLNAGIEDVTPDGFTTQQHFESINL
                     RTHRMLGDMKRCWSEFLRPALAEKDIHFWDLKDYNEAITIHLKNYFLKDIYPVLTPLA
                     FDPSHPFPHISNLSMNFAVVVNHNDQTKFARVKIPSILQRFIPIPQELTGKPGIHFAF
                     LEDVIQKNMDILFPGTQIENSYLFRVIRDTDLVIQEDEAGDLLKTVDQGLKQVRWGAV
                     SLLRIHKSMPERILNILTENFQVNAGVIHPTDGRMALGDFISLLKLHLPQLKDKPFSP
                     RMVFDEEEGDALFERIRFQDYLVHHPYDSFGNVETFIKVAVNDPSVVAIKMTLYRIGS
                     NSPLVDQLIQAVEAGKQVAVLVELKARFDERNNILWAKRLEAAGAHVVYGMLKLKTHC
                     KLCLVVRKEGDTIRRYAHIGTGNYNRSTAQIYTDLGLFTSNEQICDDVSEVFNFLTGY
                     SNQKKYRKLLVAPNSLRSGIESLIEREIQNAKEGKEARIILKINALADVKMVRMLYRA
                     SQAGVKIQMIIRGFCCLRPGVPGFSENIKISSIVGRFLEHHRIYYFFNGGQEEIYFGS
                     ADLMERNLDRRVEVVCPVLDPKIKDHIKNQILDLMLSDNVQAYDLLTDGNYKPVTPKD
                     ASETISSQIRLLDYYTNLDNTD"
     gene            3209..4618
                     /locus_tag="PLF66_15095"
     CDS             3209..4618
                     /locus_tag="PLF66_15095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65422.1"
                     /translation="MNEVKEVIWSERNLLHLKDIILNHKNLDLIPNGFNPDIYKKLKL
                     ETREFLSRKFNIDFTVKPIIANGHQPELQHPGILFKDLLVHRIAAVTDSLPLHIVVDT
                     DQFEMTYTYPEKVGDGYAHLKTLHLKDAANRIYLHRPLTQAEKEELVSILNSQKQEAK
                     SFVAKEQFESLEKILDRKIELLKTNDFLHEINEIIRSEFYTSRNIQIHTINVSELVNL
                     NSFKALVNTIRENLDAFMQVYNNSLAEYRKEHKIKNHAQPIPNLISGEMPFWILDPNT
                     NKRNVMKLDDSLDHGVILPKAVTLTMFMRLFIADFFIHGKGGGRYEEVSEHIIKDFFK
                     IEAAPYEVASATMNLNHTNQFKIPTIEEKDLDAKLRDIVYSPDKFLEPSHPLVLQKKE
                     LQSKFKNPDSDKKELHKSISSINEELSKLILPEKEKLEQLKQDFPLLQHTNQVFQSRT
                     MPFYYYYIDELIKYVQNYT"
     gene            complement(4609..6708)
                     /locus_tag="PLF66_15100"
     CDS             complement(4609..6708)
                     /locus_tag="PLF66_15100"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HMZ65423.1"
                     /translation="MLQTILRIFQNVKIKKRLFSTLLLLSIPLCFLLFFMIRTQNRAI
                     QFGKKEIVGVDYNRLLLHSLKKGRDLLGVIYTNQNTLENQKLVPILKEMEDITKTLDA
                     LDAQYGSELDTADLYSVYKEEFRKINTSISNQKTNELNIPILNFLNKTLELNSHVGDT
                     SNLILDPDLDSYYLMDISLIKIPALFAVSNEIEKLIHNTYQTKTFTEADRLQLFTLHS
                     EYQSILKQIENSYQVAFKYNPKLKLILESKKNQALVLFNQYKESVYSFSSSMSSGLEV
                     AKSEKILNSLMDYNKVLFDLYEDTVDAEKKLLEKRVTHFQIEQYTSLILVLLVVCFTV
                     FIQYMIVISITNPLLEAVSKFELLSKGNLNNKIDYYGKDEIGILSTSINHFIEYLTNL
                     IQSLTKLTNESNLIFAEISKMTSDLTLSTENQASSSEESAAALEEISSNFTQIASLIE
                     KETGDIAEIGSITDEISSSTKKASESIHNLGIIIESSAKEVKKGETVIRETVYSMNQI
                     KSAADEISKIIVLITEISKQIGLLALNASIEAARAGENGRGFSVVADEISKLSLKTEE
                     SVKHIRSLISSTNSSIKDGIHNVGTVVDVLKIVIERINETNKNAKLVDEEISSQSTNI
                     NVISSSHTKLESLSSGIDQSTKEERLAIRQVTESINRIASETQMISENTAKLREAAIK
                     IASISTEVTKTVSVFQV"
     gene            7182..7517
                     /locus_tag="PLF66_15105"
     CDS             7182..7517
                     /locus_tag="PLF66_15105"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HMZ65424.1"
                     /translation="MDLQIKVEPKYLIIYLTGPIDARTAREIEESLERIISYHQDKDI
                     IINLTDVKYISSSGLRIFISLKNSLIESTQKLKLCNLAEPVIRVFEVTRVAELFDIFK
                     TEAEALTEY"
     gene            complement(7504..8115)
                     /locus_tag="PLF66_15110"
     CDS             complement(7504..8115)
                     /locus_tag="PLF66_15110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65425.1"
                     /translation="MKTLNKFLFLLSIFFFSCISKEYRLIGITSRMEEVFYYGNDFKV
                     TAWEVDQERLDKIKLDLKNSIALENPSNAKDRWREVSELSLKDGNYILFQILPDTKVP
                     TDFLKFSFQWNGNQSTKTYSYYSEILETNIRGRYYYPIGGFYGGGGGFMYPTNSRQTE
                     QITQRLYHIYNFLLLFKNGKEGKNILKVFTPAGNQMEFELNTQ"
     gene            complement(8133..9494)
                     /locus_tag="PLF66_15115"
     CDS             complement(8133..9494)
                     /locus_tag="PLF66_15115"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HMZ65426.1"
                     /translation="MNRKTFLKFLLTISPLLKTKSLFPQTEKIKKEVLIIGAGISGLA
                     AARRLTSYGFQVTILEARNRIGGRIWTDSSLGTPVEMGAGILNGFKTNPLKKQIDQLK
                     LQSILLTLDKSEIYDTKGNQFSEEDDLKLEEYYKDFLSRLDSKKNSASKTDSLETIKE
                     DLISSMNLNQRDKYALDWLIETEIVNRYGAELKNLSLKYYDESEPLVGSDFLLVNGLS
                     ELTNSLAKGQNIKLSHIVQKIEYDKKGKVTTDKGEFSGDYIIITVPLGVLKKKKIQFQ
                     PELPQNKTDAIHKLGFGLVNKIFYKFPKKFWTKDLGNFGFIGDQNFESYEFQDLNPIK
                     NTSVLALIVSGDKAIKIEKSTKKDIAKEGVDSLKRIFGNRVSSPLEVNYSKWNTDPFS
                     FGTHSYLSIHSEPSFYDVLSESVDDILFFGGEATNKDYPSTVHGAFLSGEREAEKIIA
                     LQK"
     gene            complement(9548..9817)
                     /locus_tag="PLF66_15120"
     CDS             complement(9548..9817)
                     /locus_tag="PLF66_15120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65427.1"
                     /translation="MDLVELTKAVQDAIDKGATSVEQVHRQIANMPLDFLSKIEQLEG
                     PVSQAREFQDKTIGSIYEAIHNINAKASEIATDLLSKINSGNTTT"
     gene            complement(10033..11088)
                     /gene="bioB"
                     /locus_tag="PLF66_15125"
     CDS             complement(10033..11088)
                     /gene="bioB"
                     /locus_tag="PLF66_15125"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004777846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HMZ65428.1"
                     /translation="MIKLQEKIRSQVSSIITREEALNILEGKTNLLETVYRANMEREK
                     YFSKKVRIHILDNIKNGHCPEDCGYCAQRKNANSGVATYSLKPEEEILEDAKKAKENG
                     AYRFCIVTAGTGPNDILIDKLSSTISKITNDLGLRVCLSAGLLDEAKASKLKDAGLDR
                     YNHNLNTSESHYGEICTTHTFQDRLNTLEYVNKSDIGICSGLIVGMGESLSDLVDVAF
                     TLKRLGVISIPVNFFIPVLGHAIKNPNRLTPELCIRILSVFRLVNPDSEIRIGAGREG
                     HLKSLQSMGLFIANSLFASGYLNVKGSEVNQTIEMIRDAGFEPELEEGVLPVDLETNN
                     HLYAQENIQNLYKYKKE"
     gene            11226..11699
                     /locus_tag="PLF66_15130"
     CDS             11226..11699
                     /locus_tag="PLF66_15130"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HMZ65429.1"
                     /translation="MFSASQNNLEGMKQALALGANVNATDELECNALMFASMYGYKEI
                     VDFLLANGAKPLSSDYSSNTALLYAAQNGHTAIAEALIHAGADVNKKDDYNRTALHYA
                     ANYGYIDLILLLIRNGAELEVKNKNDNTPLDLAQISGHLEAARILKEEIDSRKYQ"
     gene            complement(11693..12976)
                     /gene="bioA"
                     /locus_tag="PLF66_15135"
     CDS             complement(11693..12976)
                     /gene="bioA"
                     /locus_tag="PLF66_15135"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016548498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HMZ65430.1"
                     /translation="MIWHPYTIQKGQPDPLKIVRAKGEFLYDINGNSYIDAVSSWWIS
                     IHGHNHPYIIERVKESLSNIDHVLLAGYTHESAEELARELIDLNQNTFKAVFYSDNGS
                     CAIDIAIKIATQYFKNLGKTKKETFLHFSQSYHGDTIGAMSVAGKSTFNSAFGNLLFF
                     SPEFTSPDCYNCPLKKNPTTCKEECLSNLEEYLKANSETTAAMIIEPIIQGAEGMKIY
                     KKEILVKLKQLAETYDVLLILDEIFTGFGRTGKNFAYEQAAIKPDIITLAKGLSGGVM
                     PLAATLVTEKIYEAFFDEDPKKAFYHGHTMTGNPTACAAGLASITLYKKEKRLEDVVR
                     LEKYFTERITDLKTKFSDKVLNPRILGSICAFELEGESNYLHPISKKIKAFSIERGVL
                     IRPLGNTIYVAPPYTIKEESLEKIFDVVQKIVETH"
     gene            complement(12973..13641)
                     /gene="bioD"
                     /locus_tag="PLF66_15140"
     CDS             complement(12973..13641)
                     /gene="bioD"
                     /locus_tag="PLF66_15140"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF024891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HMZ65431.1"
                     /translation="MAIFITATGTNVGKTVFASMLMAKYGRHFGLRYWKPVQTGALSD
                     SDLLKVKKLTGLQDKFFMDTDYHFLKAASPHYAGELEFKSVDTIRLAEKFMTQMAEPT
                     IIEGAGGLLVPLNSSTMMADILKKAKIPVILISSPVLGTINQTLLSIEAMQHRDIPIL
                     GFYMFGEKNDLSRNNIFTIQEFSGVKCLGELGIPASVSEGKPFLQYVDTYFDTTETIK
                     NLLK"
     gene            complement(13644..14807)
                     /locus_tag="PLF66_15145"
     CDS             complement(13644..14807)
                     /locus_tag="PLF66_15145"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HMZ65432.1"
                     /translation="MHKRLFYLDNLKTFALLLGVLFHTSIVYAPEIGYAIKNPERNYF
                     FSVIVHFIHVFRMPLFFFLSGFFSFMVLNKHGGRNFTFSRFERMFAPMIVGLLFFSPI
                     QYFIVYNQKNNPISFLEYYPLFFTPNEFDLSHIWFLVYLFLFSFLLLLNHKFHIGKLL
                     AKILFKNSFLNKIIQFKKTNSLNLFIRFVFLSFASIYLINFLFNKDDSFLRIQPVSFI
                     YYLSFFLIGVYAYKNEILQNPDITFGSKLKPIFRLIRFIVLFLIYLYLNETDPYWMSF
                     AYDSKRILWRGVHLLIDSSLAWILIFELAPFFKRYLDHTGPILDHLRNSGMSVYLIHH
                     PISLLIGYFLLSVEISVFVKFSIHTISVYFLSFFFYYYVIKKSFILNRVFGTK"
     gene            14856..16700
                     /locus_tag="PLF66_15150"
     CDS             14856..16700
                     /locus_tag="PLF66_15150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010415254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HMZ65433.1"
                     /translation="MKNRFNLFIVFIFFYFPLFAKEVVIVGNIIGAGSYIETDDNTYP
                     FKHTELQKELSELTGKKVRMLCLFEEDSCVPLRYEIAPFANDKNLAKWTIKKIPKYVY
                     RGLIAFNPSVTPDGNILFWTVLTPEGHGSNTQKIWYSEMDTHGFWKKGVQMDPPLNNK
                     APAAIISALPGGNELFVFGSHADQESFEEIKRQMDAEKAEIVKTSKSAREVEGRYAAV
                     RERYRREMEKIQNKVPLYKSFKQGNGWSVPQRIPFPDFYNLYRSEDNANLQIFGGSTL
                     SSSGKTLIYSAKHKDSVGKLDLYVSNITDGVFPIGTNLGKEINTEYEEMAPFLASDDR
                     TLYFSSNGHSGLSIYFTQRVGDSWTEWSHPQEISKNLKGVNFFSIPASGNWAYASREG
                     QLLMTYLPNETKPNPVIIVKGRITNDKGVPLGAEVYYESLTTKENKGSTISDPNTGKF
                     SIVLPYGDNYGFHAKKEGYLPFHRSKDLRESEKLYQEVEVDMILPKIEKGGEITINNL
                     FFESNRSEIKKESEPELDRLGEIMKMNKNLEVALEGHTDNVGKSADNIALSLARANAV
                     AEYIVKKFGIEPSRLKVEGRGEEAPLTDNNTADGRAKNRRVVFRILKN"
     gene            16773..17726
                     /locus_tag="PLF66_15155"
     CDS             16773..17726
                     /locus_tag="PLF66_15155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65434.1"
                     /translation="MRFRFLCLYFVSNLLLFSEELNRQYVLDAYSLRKESFGLGIQKK
                     ENADSLWTWYHFAKEFRNSFRFALQLSAISHSKKKLEVDFFSSKYQFPFSSSEQLITI
                     SPGFRYSYIHFYTKFYSNTSGRYDAILGLWLYKEKSVLLSYSKNSKEQSHSFSFSILT
                     GKNPTIALGLGKEYKGGNTEWIVNLGVSFGFDSYETGISYLPRTEKEDSGTFVFASRS
                     LKEKEESLFGEEMNPYVDNGHLPEKREQVAEIRIKKRERVVYEISIDELLKFRIPLAI
                     AIRIHRASKSKEDYLELLKRLPADLVRKCNKIQFDKARKKN"
     gene            17723..19006
                     /locus_tag="PLF66_15160"
     CDS             17723..19006
                     /locus_tag="PLF66_15160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65435.1"
                     /translation="MNRFLYYFLLFLFVGKIYSEESRPRIIFYGTTEYFESRYVKDNQ
                     RTKTNFYVGSVYKNWQGAVEKRGETPLASMEFKNKYFYFSAGNRYKPISGFYILRNEK
                     YYSAFQNPRLGLIEQPLKRSYWMGVNPFNWSAGFFIGEDMAVKRPAFYFKSPENLLAY
                     TYSPETKIHFIAANIRDYRPKSLNKMEFNLSSQLLGKRENYFGYFNFRVFEKEKGLET
                     ELSLYKEDNGGLFVSNQDNLKETSSEKAVFLKISKYHYNRVEIFQTETKALRERRIGI
                     NSSLFSGKYGAICVSGRGYEDVKSLSDKREISTTIGVGISYEYRFKSTEFMFRLEQRK
                     NQDELAELKWTIRPIVDWKLEISSLLQKDSNQFRSLYEQWSDGENINTILTNRSAAFK
                     LKLVSSFLVFNVSGSRRPNGTGEVYFANIQMKQEF"
     gene            19720..20919
                     /locus_tag="PLF66_15165"
     CDS             19720..20919
                     /locus_tag="PLF66_15165"
                     /inference="COORDINATES: protein motif:HMM:NF025253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HMZ65436.1"
                     /translation="MNTENKEQSSLNESIDKVGTYFSKQKQVIKESLFKGAFQGVGFL
                     LAIGVTTVLAVAVTGTINTFSSGGVIKSADINTNFTTLKTAIEGITSSQWTTNGANIG
                     YTAGNVGVGTTSPSEKLSVKGTGSFSDSNTVASNFLNVASDTAAINIITHGSNYGSTL
                     YGVSRNNMGLIEGQSNSAFVIGTSNTAPIIFGVNRTENMRIGTNGNVGIGTTSPSDNF
                     SVGNKVLISSSSSPSNPPAGGAVLFMNVGNDLIAKDSTGQNLNIGDFTASDKRLKKDI
                     IPIIGALDKIKELSAVYFNWDRSVKRVSDYSEKRQLGLIAQEIEKQIPEIVSIDSFGY
                     KRMEYTKMIPVLVSATQEQQKEIERLSSNLISAREENSQLRKRLERVESVLAAVERQS
                     NEKMVRK"
     gene            21212..21538
                     /locus_tag="PLF66_15170"
     CDS             21212..21538
                     /locus_tag="PLF66_15170"
                     /inference="COORDINATES: protein motif:HMM:NF016167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HMZ65437.1"
                     /translation="MTFLEIEKEIKNLHSGEKAQLFHLLAMDISKNFPGIEKLQGVCG
                     GSACVVRTRIPIWSLEYCRRNGLSEAQILFDYPTLRAADLVSAWGYVSLNQLEIDKEI
                     QENLDA"
     gene            21538..21876
                     /locus_tag="PLF66_15175"
     CDS             21538..21876
                     /locus_tag="PLF66_15175"
                     /inference="COORDINATES: protein motif:HMM:NF037832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HMZ65438.1"
                     /translation="MARFYCNENFRYSVVLELRKLGHDVLTSLEAGNANKSIPDEDVL
                     SFSINERRILLTFNRKDFIRLHKERSTHFGILVCTDNPDNIFLANKIHELVLSQEIVN
                     KLIRNNRPNS"
     gene            complement(22411..22473)
                     /locus_tag="PLF66_15180"
     CDS             complement(22411..22473)
                     /locus_tag="PLF66_15180"
                     /inference="COORDINATES: protein motif:HMM:NF016167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65439.1"
                     /translation="MLEDYPDLEKDDLRACILFA"
     gene            complement(22467..22607)
                     /locus_tag="PLF66_15185"
     CDS             complement(22467..22607)
                     /locus_tag="PLF66_15185"
                     /inference="COORDINATES: protein motif:HMM:NF016167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HMZ65440.1"
                     /translation="MNSENLLKRITLNPEVCFGKPTIRNLRYPVELMLELLSGEMTKK
                     KF"
     gene            complement(22650..23243)
                     /gene="recR"
                     /locus_tag="PLF66_15190"
     CDS             complement(22650..23243)
                     /gene="recR"
                     /locus_tag="PLF66_15190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010577450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HMZ65441.1"
                     /translation="MTDSLFKKLVGALSSLPGIGKKSAYRLGFYILRMDSSSFQNFID
                     TIETVKSSLKFCKKCGGITEFEVCEICSSDKRQTNIICIIENPEDIFFIENTGEYKGK
                     YHVLNGVISPLDGIGPENLRIKELLERLKEEPFEEVLIATNPTLEGDATASYLFNLIK
                     PIGLKITRIAHGVTVGSTLEYADQYTLGKAIRARLTI"
     gene            complement(23236..23586)
                     /locus_tag="PLF66_15195"
     CDS             complement(23236..23586)
                     /locus_tag="PLF66_15195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004765668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HMZ65442.1"
                     /translation="MFGKKLDKMAETLSKMKDMKNQMAAIQKRISVIRVTASAGAGMV
                     DVTASGDGIITNVKINKNLFEGDDMKMLEDLIISATNEALKKSKDAMTHEMKNVTGGF
                     DPNELSKLFGGSGD"
     gene            complement(23597..24949)
                     /gene="dnaX"
                     /locus_tag="PLF66_15200"
     CDS             complement(23597..24949)
                     /gene="dnaX"
                     /locus_tag="PLF66_15200"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002102006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HMZ65443.1"
                     /translation="MSEESHLVFARKYRPQLFKEVIHQDLAIGALMNALKNNRVGHAY
                     IFFGPRGVGKTTIARLLAKRLNCESPEDNEPCNKCKSCTEIMKGISNDVLEIDAASNR
                     GIDNIRDLRENVKFSPMGGRYKIYIIDEVHMLTDQSFNALLKTLEEPPHHVVFILATT
                     EFHKIPETILSRCQDFVFKKVPLPNLQSYVEHLCKIEKIDYDADGIFWVAKKGDGSVR
                     DTLSFLEQAVTFTDGHITGKQIGKMIGYQGVEVQIEFLKSILGEKTFHKSFEMIEKIY
                     NEGTDLHKFAWEFVEFTHALIIIKENLADRDTLNYPIEDIEKISKELEPFSVEVLTLL
                     SEKIYSMYEKLMYLKLRNSFEMKIFIEISIRKLIFDMNKPSVAGVLEKIHDLYQILQK
                     EGERYTASPRQSNPVAAKENLPQKGNTEVLQKEEIDVESILKDKFLGTEVDSSKLPEI
                     "
     gene            complement(24961..25044)
                     /locus_tag="PLF66_15205"
     tRNA            complement(24961..25044)
                     /locus_tag="PLF66_15205"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(25008..25010),aa:Ser,seq:gga)
     gene            25249..25761
                     /locus_tag="PLF66_15210"
     CDS             25249..25761
                     /locus_tag="PLF66_15210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002974835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HMZ65444.1"
                     /translation="MKKIYLAGPEVFLPDSFAVLNQHKEICKRYGYLGLSPFDGEVTT
                     EVGLHRAKKIYLENCKLIREADIVLANCNSFRGALMDDGTAFEIGYAKALGKKIFGYI
                     NKREPLPNLVEKVVPTVAHPSGYRMDSQGYLLNEDFGNSINLMLEFSILESGGELVEG
                     SFEDALKRIV"
     gene            complement(26169..26639)
                     /locus_tag="PLF66_15215"
     CDS             complement(26169..26639)
                     /locus_tag="PLF66_15215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65445.1"
                     /translation="MIILQIKEEMKLRKFEEALSLIEQIPENFMTSEILILKGLCIQL
                     SENLKYDLKDAELSLKKAIELDSNNLDAKMELGWFYYNVLNESEESYHLFGQALEDIQ
                     SKLTELLEGFMKINLESSSYFDAKQFIVNYTNLILQNEKIKILIADNESLRESI"
     gene            26860..27279
                     /gene="rnpB"
                     /locus_tag="PLF66_15220"
     ncRNA           26860..27279
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PLF66_15220"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(27274..>28828)
                     /locus_tag="PLF66_15225"
     CDS             complement(27274..>28828)
                     /locus_tag="PLF66_15225"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HMZ65446.1"
                     /translation="TLCFLPLIFYFITLIGYAETYLRLYEFRIFTGDRIGAEKMKVFA
                     LEAATKAIDLAPQSSIAHRAMSFYYFYLDPDFEKSKFHAQKAVELDPKDPEAAMRLFV
                     VKTKENKKLLSPDNKDLLSIYTLNPNLISTNVYLAKAYRLAGNYTKAIEQYQKILEIS
                     PKTISIYAEISSVFADQEKWEEALQWLDKADEIEPNGFQVQIHYANYYIRKKEYKKAN
                     EYLTLAQKIQPKSTIPYEALANIALREKNYEEATTLLQKCIELDPKKGSFYNLLGNVY
                     KSRNNFQMAIINFQKATKVEPKLTLAYENLANLYLEQNRIDMAIKSYEKCIEIEPSNS
                     YYLRSIAEVYITKKDYESAISYYNKAQEADPNEYMIYNELGSTYVLKKEYEKGKEKFT
                     RAVELGNEEVKSYGHKGLADLKKKQRKFEEAIDLYKKAISLHSANIEASYGLANAYSK
                     LGNNQEAIVVLKSVIESHPEMGKAYYELGKYLQAEKRSDEANESFKKACELGEAKSCN
                     PPKEKKKKK"
BASE COUNT         9240 a         4620 c         5122 g         9846 t
ORIGIN      
        1 gaacttgctc cagaactaac ggttgccccg tttatcgtta tacgggctgt ggtatccgag
       61 gaagtaggag taaactgaat ggatgaaacc gagtttccaa cactcattgt gtaattgaaa
      121 acagtcgagg aaaaagaagg agagagagtt cctgtagacg ggacaagatt cgtcaaagac
      181 ccactcgttg tagctgtatt cgacgagttg gaattaagaa gtggtaaaac caatttaatc
      241 ttgaagctat catcatgact acagtttagg atgaatgcta gaaacatgat aatcaataag
      301 aaatgcagtt ttctaataaa ccaaatcatt ttaatctctc ctaacttaat agactattga
      361 attgaaaaga aaattccaat tcaattggat aagtaaattt ttaaattata taccagataa
      421 caaaagtaat ttcccaaaag gattttattt gagtcgcatt tggtataccg aacatatgat
      481 gttcgggaac actgagaagt gtttagagca aacgcatctt attattttta aaatgggact
      541 tttcttttgg cgtctgtcta aatcaagatc agtgagtagt tgattctatt taggattaaa
      601 aaaagagaaa ggactttttt agatttaaaa aatttccttt ctctctgcat attttacttg
      661 gacttaacgt ttgacgatta gtagaaatac ctaaattaat ccgtattatc taggttagta
      721 taatagtcta agagtcggat ttgagatgag attgtttcac tagcatcctt tggggtaaca
      781 ggtttgtaat ttccatctgt aagtaagtca tatgcttgga cattatcact taacatcaaa
      841 tctaaaattt ggttcttgat atgatcttta atctttggat caagcacagg acaaaccact
      901 tccacacgtc tgtctaaatt tctctccatt agatctgctg aaccaaaata aatttcctct
      961 tgtcctccat tgaagaaata ataaattctg tggtgctcta aaaatcttcc tacaatggat
     1021 gaaattttta tgttctcact gaatccgggc acaccaggac gtaaacaaca aaagcctcga
     1081 atgatcattt gaatttttac acccgcttga gatgctctat aaagcattcg aaccatcttt
     1141 acatcagcga gagcattgat ttttaaaatg atccttgctt cttttccttc ttttgcattt
     1201 tgaatttctc tttcgattaa agactctatc ccagagcgaa gtgaattggg agccactaag
     1261 agctttctgt attttttctg attggaatat ccagttagaa aattaaatac ttcggaaaca
     1321 tcatcacaga tttgttcgtt gcttgtaaag agtcctaggt ctgtatagat ttgagcagtg
     1381 gatcgattgt aattgcctgt tccaatatgt gcataacgtc taattgtatc gccttctttt
     1441 ctcacaacca gacaaagttt acaatgtgtc tttagtttta gcattccata gactacatgt
     1501 gctcccgctg cttctagtct ttttgcccat agaatattat tcctttcatc gaacctagct
     1561 tttaattcaa ctaaaactgc aacttgtttg cccgcttcaa ctgcttggat caattgatcc
     1621 acaagaggag aattcgaacc gattctatat agagtcattt taattgcgac aacggaggga
     1681 tcattcactg ctacttttat aaatgtctct acatttccaa aagaatcata aggatgatgc
     1741 actaaataat cttggaagcg aattctttcg aataacgcat ctccttcttc ttcatcaaaa
     1801 accattctgg gagaaaatgg tttgtctttg agttgtggta gatgtagttt taacagtgat
     1861 ataaaatctc caagagccat tcttccatct gttggatgaa tcactcctgc atttacttgg
     1921 aagttttctg taagaatgtt aagaattctt tcaggcatag atttatgaat tcttaaaagg
     1981 gaaactgctc cccagcgaac ttgttttaag ccttgatcaa ctgtctttaa tagatcgcct
     2041 gcctcgtctt cttgaatcac taaatcagta tcacgaatca ctctaaataa ataactattt
     2101 tctatttgcg ttccaggaaa aagaatatcc atattttttt gaatcacatc ttctaagaat
     2161 gcaaagtgaa ttcctggctt gccagttaat tcttgtggaa ttggaataaa tctctgtaaa
     2221 atacttggta tttttactct tgcaaatttt gtttgatcgt tgtgatttac tacaaccgcg
     2281 aaattcatac ttaaattgga tatatgtgga aatggatgac ttggatcgaa tgcaagcggt
     2341 gttaaaacgg gatagatatc ttttaaaaaa taatttttca aatgaatcgt aattgcttca
     2401 ttataatctt ttaaatccca aaaatgaatg tctttttctg cgagtgctgg tctcaaaaat
     2461 tcactccaac atcttttcat atctcctaac atacgatgtg ttctaagatt gattgattca
     2521 aaatgttgtt gtgtagtaaa accgtcaggc gttacatctt ctatgcctgc atttaatttt
     2581 tttaaaatag tagaaagtct taccatgaaa aattcgtcta gattgctact gactatggat
     2641 aagaatttaa ctctctctaa gagtggatga gattcattat aagcttgatc tagaactctc
     2701 tgattgaact caaaccaact gagttctcta cttatatgca attcaggatg atctaaatta
     2761 tttgggtcta gtttactcca atcgaattgg agttcatttt tttcgatcat gatattctca
     2821 cttcaaattt tttctagttc caatatgaaa cattctagaa atttgtaaaa tggaaataaa
     2881 gaaatagaaa taaaaagtaa aaattgattg aatactaagt ttggtttaat catttgcttc
     2941 ccacaaacaa aaaatgaatt gagttttatc aaaacatttc cctttcagta gtgtgaatcg
     3001 aaattttgaa gtatagaacg taacgttatt ttccttgccc aattagaagt atttatattc
     3061 cggaatggaa tgattgattg atctatgcag attgagcact tttttttaag tagcccttaa
     3121 aaagagaaga tattttgcag gagacttgtc ttctttaaga acttgacagt agaaatcaaa
     3181 agaaagctaa gtatagatag gcaaaaaaat gaacgaagta aaagaagtaa tttggagcga
     3241 gcgaaatctt ctccatctaa aagatataat tctaaatcat aaaaacttag atttaattcc
     3301 aaatggtttt aacccagata tctataagaa attaaaacta gaaacgagag agtttctatc
     3361 tcgtaaattt aatatagatt ttaccgtaaa accaatcatt gccaacggtc atcaacccga
     3421 gctacagcac ccaggaattc tatttaaaga tttactcgtt catcgaattg ctgcggttac
     3481 agatagttta cctctgcaca tagtggtaga tacagaccag tttgagatga cctataccta
     3541 tccagaaaaa gttggagatg gatatgctca tttgaaaaca ttgcatctaa aagatgcggc
     3601 taaccgtatt tatctgcatc gtcctctaac tcaagcagaa aaagaagaac ttgtatctat
     3661 tttaaattcc caaaaacaag aagcaaaatc ctttgtcgca aaagaacaat ttgaatctct
     3721 agagaaaatc ttagatcgaa aaatagaact tctaaagaca aatgattttt tacacgaaat
     3781 caatgaaatc attcgaagcg aattctatac aagtagaaac attcagattc atactatcaa
     3841 tgtaagtgag ttagtgaatt taaattcatt taaagcgctt gtgaatacga ttcgagaaaa
     3901 tttagatgcg tttatgcaag tgtataataa ttcacttgct gaatatagaa aagaacacaa
     3961 gattaaaaat catgcgcaac ctattccaaa tttaatttct ggagaaatgc ctttttggat
     4021 acttgatcca aatacaaata aaagaaatgt tatgaaatta gatgattctc tagaccatgg
     4081 agtgattttg cccaaagcag ttactctcac aatgtttatg agattattca tagctgattt
     4141 ttttattcat gggaaaggag gaggacgtta tgaggaagta tcagaacata ttataaaaga
     4201 tttctttaaa atagaagcag cgccctatga agtagcaagt gctactatga acttgaatca
     4261 caccaatcaa tttaaaattc caacaataga ggaaaaggac ttagatgcaa agctaagaga
     4321 cattgtgtat tctcctgata agtttttaga gccatcgcat cctcttgtgt tacaaaaaaa
     4381 agaattacaa tctaaattta aaaatccaga ttcagataaa aaagaacttc acaaatcgat
     4441 ttcgagcatc aatgaagagt tatcaaaact aatattacct gaaaaggaaa aattagaaca
     4501 actcaaacag gattttccgc tcttgcaaca tacaaatcag gtatttcaat ctagaacaat
     4561 gcctttctat tattactata tagatgagct cattaagtat gttcaaaatt atacttgaaa
     4621 tacactgact gttttagtga cttctgttga tatagatgca attttaattg cggcttctct
     4681 cagctttgct gtgttttctg atatcatttg agtttcacta gcaatacgat ttatactttc
     4741 tgtcacttgc cgaatcgcta gtctctcttc ttttgtgctt tgatctattc ctgaagaaag
     4801 actttctaat tttgtgtgac tactagaaat aacgtttatg tttgtagatt gagaagatat
     4861 ttcttcatct actagttttg catttttatt tgtttcattg attcgttcta ttactatttt
     4921 tagaacatct acgacagttc ctacattgtg tattccatct tttattgaac tatttgtaga
     4981 tgagattaaa gaacggatat gttttacact ttcttctgtt tttaaagaga gcttggaaat
     5041 ttcatctgct accacagaaa atccccttcc attttctcct gctcttgccg cttcaatgct
     5101 tgcattcaat gcaagtaatc caatttgctt cgaaatttct gttattaaaa caatgatttt
     5161 actaatttca tctgctgctg atttgatttg attcatagag taaacagtct ctctaatcac
     5221 tgtctctcct ttttttacct ctttagcaga actctcaatt atgattccaa gattgtgtat
     5281 ggattcagat gcttttttgg tagaggaaga tatttcatct gtgatagaac ctatttctgc
     5341 aatatcacct gtttcctttt caattaaact tgcaatttga gtgaagttag acgatatctc
     5401 ttctaaagct gctgccgatt cttcagagct agatgcttga ttctctgtgg atagagttag
     5461 atcggaagtc attttggaga tttctgcaaa tataagattt gattcatttg ttaatttagt
     5521 cagtgattgg attaagtttg ttaggtattc aataaaatga tttatggagg tagataaaat
     5581 tccaatttca tctttgccat aataatcaat tttgttattt aaatttccct tggaaagtaa
     5641 ttcaaatttg gatactgctt ctagtaaagg gtttgtgata ctgattacaa tcatatattg
     5701 aataaagact gtaaaacaaa caaccaataa gactaaaatt aaagaggtgt attgttcaat
     5761 ttgaaaatga gtaactcttt tttctaaaag ttttttttct gcatctactg tatcttcata
     5821 taaatcaaat aataccttgt tataatccat taacgaattt aaaatttttt ctgatttagc
     5881 cacttctagt ccagaactca ttgagcttga aaaagaataa actgattctt tatactgatt
     5941 gaataggact agagcttgat tctttttaga ttcaagaatt aattttaatt ttggattgta
     6001 tttaaaagcg acttgatagg agttttctat ttgcttaagg atagattggt attctgaatg
     6061 aagcgtaaaa agttgtaatc tatctgcttc tgtgaatgtt tttgtttgat atgtgttgtg
     6121 aatgagtttt tcgatttcat ttgagacagc gaataacgct ggtattttaa ttaaagatat
     6181 atccattaga tagtagctat ctaaatcagg atctaaaatt aaattggatg tatctccaac
     6241 atgtgaattt agttcaagag tcttatttaa aaaatttaag ataggaatgt ttaattcgtt
     6301 tgtcttttga tttgagatag aagtattgat tttcctaaat tcttctttat aaacggaata
     6361 taaatcagct gtgtctagtt ctgatccata ttgtgcatcg agagcatcta aagttttagt
     6421 tatgtcttcc atttctttta gaattgggac taatttttga ttctcaaggg tattctgatt
     6481 tgtatatata acaccgagta ggtctcttcc cttttttaag gaatgtagaa gcaaacggtt
     6541 gtaatccacg ccaactattt ctttttttcc aaattgaatt gctctatttt gagtgcgtat
     6601 cataaaaaaa agaagaaaac aaagtggaat cgaaagtaaa agcaatgtgg agaaaagtct
     6661 ttttttaatt tttacattct gaaatattct aagtatagtc tgtagcacgg gataatcatt
     6721 tccgagctat caacaaatta caagcaattt agatagaaga ttagctatga aatctaattt
     6781 tctcaagtgt atgtatcctt gaattaaaga gtatattctt aaaaaaataa aaaaatgaca
     6841 aattatctct aacgttatct cttcttattt tctttttttt agaattagta acagtgggtt
     6901 ctttttattt tctagaaatg aatggataca aagcggaatt gggatctaat agaaatgtat
     6961 atattttcta tacttttaat ccttctttat ttagaacacg attcttgaga gagaatccat
     7021 aatcagattc aaaattcaat catacttcaa tttttgtttt tgcactttga aaatttggga
     7081 ttcaagcatt tagcactaat tttactgaaa aataagatat ttaggttaga ttgaaaccca
     7141 ttttaggatt gtataattgg atattcctat aaaattgacg tatggatttg caaattaagg
     7201 tcgagccaaa atatttaatc atctatttga caggacctat tgatgctagg actgcacgag
     7261 aaatagagga aagtcttgaa aggattattt cctatcatca ggacaaggat ataattataa
     7321 atcttacaga tgttaagtat atctcttctt ctggactgcg tatttttatt tcccttaaaa
     7381 acagtttgat cgagtccaca cagaagttaa aactttgtaa tttagctgag ccagtgattc
     7441 gtgtttttga agtaacacga gttgcggagt tgtttgatat ttttaaaaca gaagcagaag
     7501 ctcttactga gtattgagtt cgaattccat ttgattcccg gccggtgtga atactttcaa
     7561 aatattcttc ccttcttttc cattcttaaa taataataaa aaattataaa tatgataaag
     7621 acgctgcgtt atttgctctg tctgtctaga attcgttgga tacataaagc caccaccgcc
     7681 tccataaaat ccaccgatag ggtaatagta tcttcctcga atgtttgttt ctagaatttc
     7741 actataatag gaatatgttt tagtggactg gttgccattc cactgaaaac taaatttcaa
     7801 aaaatctgtt ggcactttgg tatcaggaag gatttgaaat aatatgtagt ttccatcttt
     7861 taaggaaagt tcggaaactt ctctccacct gtctttcgca ttggaaggat tttctaaagc
     7921 aatgctattt tttaaatcta gttttatttt atcgagtctt tcttgatcta cttcccaggc
     7981 agtgacttta aaatcatttc cataatagaa aacttcttcc atacgggagg taatcccaat
     8041 tagcctatac tcttttgata tacaggaaaa gaaaaagatt gaaagtaaga atagaaattt
     8101 gtttagagtc ttcataggtt taaaaaatgt ttttattttt gcaatgcaat tattttttca
     8161 gcctctcttt ctccacttaa aaaagcgcca tggacagttg atggataatc cttgttagtt
     8221 gcttctccgc cgaagaataa aatatcatct actgattctg aaagcacatc gtaaaaggat
     8281 ggctctgaat gaatgcttag ataggaatga gtgccaaaac taaacggatc tgtattccat
     8341 ttagaataat ttacttctag tggagaggat actctatttc caaaaattct ttttaaggaa
     8401 tcgactcctt ctttagcgat atcctttttg gtagattttt ctatttttat agctttgtcc
     8461 ccagatacaa tcaaagcaag aactgacgta ttcttaattg gatttaaatc ctgaaattcg
     8521 taagattcaa aattttgatc tcctataaaa ccgaaatttc ccaggtcttt tgtccaaaat
     8581 ttctttggaa atttataaaa gattttattt actaatccaa aacctaattt atgaattgca
     8641 tctgttttat tttgtggtag ttcaggttga aattgtatct ttttcttttt gaggacaccg
     8701 agaggaactg ttatgatgat atagtcgcca ctaaattctc ctttgtccgt tgtaaccttc
     8761 ccttttttgt catactcaat tttctgaact atgtgtgaca attttatatt ttgacctttc
     8821 gcaagagaat tggttaattc ggataagccg tttaccaaaa gaaagtctga gcccacaagt
     8881 ggttctgatt catcgtagta tttgagagat agatttttta attctgcgcc atatcgattt
     8941 acgatttctg tttcgatgag ccaatcgaga gcatatttat ctcgttggtt tagattcata
     9001 ctcgatatta aatcttcctt tatggtttct agtgaatccg ttttagaagc agaatttttt
     9061 tttgaatcta atctagatag aaaatcttta taatactctt ctagttttaa atcatcttct
     9121 tctgaaaact gattcccttt tgtatcatag atttccgatt tatctaaagt aagaagaatg
     9181 ctttgtagtt ttaattggtc tatctgtttt tttaagggat ttgtcttaaa tccatttagg
     9241 attcctgctc ccatttccac tggtgttcca agagaggagt ctgtccagat tcttcctcca
     9301 atcctatttc tagcttctaa tatcgttacc tgaaatccat aagatgttaa tcgtctagct
     9361 gcggctaatc ctgatatacc agcaccaata attagaactt ctttttttat cttttcggtt
     9421 tgagggaata gagattttgt ttttaaaaga ggtgagattg tgagtaaaaa tttgagaaat
     9481 gtttttcgat tcatattttt aagtcattgt cattttaaat caaataacaa tgactgttta
     9541 caactaatta ggttgttgtg tttccagaat taattttaga gagtaggtct gttgcaattt
     9601 cacttgcttt tgcattgata ttgtgaattg cttcatagat actgcctatc gttttgtctt
     9661 gaaattctct tgcttgagaa acaggtcctt ctaattgctc aattttagat aaaaaatcga
     9721 gtggcatatt tgcaatttgt ctgtgaactt gttctactga cgttgctcct ttatcgattg
     9781 catcttgaac tgcttttgtt aattctacta aatccattgg ttaccttcct tttgacacag
     9841 ctaagcaaat agaaagaaaa tgagcaagtt ctttttcgat cagcaatgtt gtttcaaaac
     9901 gtattccttt tgattacaaa tacgaaaact tagaagcctt tagaaataga actaatactt
     9961 ttgtgagatt tagacagaac caagagtttg agtttttctc tcttcccaaa cccctgattc
    10021 tattgcttcc atttactctt ttttgtattt gtataggttt tgaatgtttt cctgtgcata
    10081 cagatgattg tttgtttcta gatccaccgg aagaactcct tcctctaatt caggctcgaa
    10141 tccagcatct cgaatcattt caatcgtttg gtttacttca cttcccttca cattcaggta
    10201 tccagaggca aaaagggaat ttgcaataaa taatcccatc gattgtagac tttttaaatg
    10261 tccctcgcgt cctgccccaa ttcgaatttc tgaatctgga tttactaaac ggaatactga
    10321 taatattcga atgcatagct ctggagttaa gcgatttgga tttttaattg catgtccaag
    10381 aactggtata aaaaagttta cgggaattga gattacaccg agtctcttta aagtaaatgc
    10441 tacatctact aaatcagata ggctctctcc cattcctaca atgagcccac tgcagattcc
    10501 aatatccgat ttgtttacat attcgagagt gtttaatcta tcttgaaatg tatgagttgt
    10561 gcaaatctct ccataatgag attccgatgt atttagattg tgattgtatc ggtctagacc
    10621 tgcatctttg agtttgcttg cttttgcttc atctaagaga ccagcagata aacaaactct
    10681 taagcctaga tcatttgtga tttttgaaat agtagaagaa agcttatcta ttaaaatatc
    10741 atttggaccg gttcctgctg taacgataca gaaacgatag gcgccatttt cttttgcttt
    10801 ttttgcatct tctaatattt cttcttctgg ttttagagag taggttgcta cgcctgaatt
    10861 tgcatttttt ctctgtgcac agtagccaca gtcttcagga caatgtccat tttttatatt
    10921 gtctaaaata tgaattcgga ctttttttga aaaatatttt tctctttcca tattggctcg
    10981 gtaaacggtt tcaagtagat ttgtttttcc ttctagtata tttagggctt cttctctggt
    11041 aataatgctc gatacttgtg atctaatttt ttcttgcagt ttaatcataa ttggctataa
    11101 ttgaatcaag tttctgaaat acaagagaaa tttaaggaga gaaagccatg aagtttttaa
    11161 aagtatttac cctaattttc atcctaagtt cctctctact cttatcctag ccaaatgaag
    11221 attttatttt ttctgctagc caaaataatt tggaagggat gaaacaagcc ttagcattgg
    11281 gtgccaatgt aaatgcgaca gatgaattgg aatgcaatgc tctcatgttt gcttctatgt
    11341 atggatataa agaaattgta gattttttat tggctaacgg tgctaagccg cttagttctg
    11401 actattctag taatacagct cttttgtatg cagctcagaa cgggcataca gcaattgcag
    11461 aggcattgat tcatgcagga gccgatgtaa ataaaaaaga tgactacaac cggactgctc
    11521 tgcactatgc agcaaactat gggtatatag atttaattct tttactcatt cgaaatggag
    11581 ctgagttaga agtaaaaaat aaaaatgata atactccgct cgacttggcg caaataagtg
    11641 gtcatttaga agcagctaga attttaaaag aagagatcga tagtcgaaag tatcaatgag
    11701 tttctacgat cttttgaaca acatcaaaga ttttttctag agactcttct ttgattgtat
    11761 aagggggagc aacatagatt gtatttccaa gaggtcgaat caatactcct ctttcaattg
    11821 aaaatgcctt tattttctta gatattggat gcaggtaatt ggattctccc tctaattcaa
    11881 aagcacaaat agatcctaat attcttgggt ttaaaacttt atcactgaat ttcgttttta
    11941 aatcggttat tctttcggta aaatattttt ctaaacgaac tacatcttct aatctttttt
    12001 cttttttata aagagtaatt gaggcaagtc ctgctgcaca agcggttgga ttccccgtca
    12061 ttgtgtgacc gtgataaaaa gcttttttgg gatcttcatc aaagaatgct tcatagattt
    12121 tttctgtaac aagtgttgca gctaatggca ttactccgcc agatagacct tttgctagag
    12181 tgatgatatc tggttttatt gcggcttgct cataggcaaa gttttttcct gttcttccaa
    12241 atcctgtaaa aatttcatcc aaaattaaaa ggacgtcata ggtttccgct aattgtttta
    12301 gttttactag gatttctttt ttatatatct tcattccctc cgcaccttga attataggtt
    12361 ctataatcat cgcggctgtg gtttccgagt ttgcttttaa gtattcttct aaattagata
    12421 gacattcctc tttacaagtg gttgggtttt tctttagagg acaattgtag caatcaggag
    12481 aagtaaattc aggggagaag aaaagtaaat ttccaaacgc tgagttgaat gtagattttc
    12541 cggcgacaga catagcgcct attgtatcac catgatagct ttgactaaaa tgtaaaaaag
    12601 tttccttttt ggttttacct agatttttaa aatattgagt tgctatttta attgctatat
    12661 cgattgcaca ggatccattg tcagaataaa aaactgcttt gaatgtattt tgattgagat
    12721 caattaattc ccttgcgagt tcttcggctg attcatgtgt gtaaccagca agcaaaacat
    12781 gatcgatatt cgaaagactc tcttttactc tctcaattat atagggatga ttatgaccat
    12841 ggatgctaat ccaccaagag gacactgcat ctatgtagga attcccattt atgtcgtata
    12901 gaaattcccc tttagcacga acgattttaa gtggatctgg ctgtcctttt tgaatcgtat
    12961 agggatgcca gatcatttta aaagattttt aattgtttct gttgtatcga aataagtgtc
    13021 aacatattgc aagaatggtt tgccttccga tacacttgca gggattccta gttcgcctaa
    13081 acattttact cccgaaaatt cttggatggt gaaaatatta tttctagata gatcgttttt
    13141 ttcaccaaac atgtagaaac cgagaattgg aatatcccta tgttgcattg cttcaatgga
    13201 gagaagagtt tgattgattg ttccaagaac aggagaagaa attaaaatga caggaatctt
    13261 tgctttcttt aaaatgtccg ccatcattgt gcttgagttt aacggaacta aaagaccacc
    13321 tgcaccctct atgattgtag gttctgccat ctgcgtcata aatttctctg cgagtcgaat
    13381 ggtatctaca cttttaaatt ctaattctcc ggcatagtga ggagaagcag cttttaaaaa
    13441 atgatagtct gtatccatga aaaacttgtc ttgtagacca gttagctttt ttacttttaa
    13501 taggtctgaa tcactcaatg cacccgtttg aactggcttc caataacgaa gaccaaaatg
    13561 ccttccgtat tttgccatta acatagatgc aaaaactgtt tttccgacat tcgttccagt
    13621 tgccgtgata aatattgcca tatctactta gttccaaaca ctctgtttaa aataaatgat
    13681 ttttttatta cgtagtagta gaaaaaaaag ctcagaaagt agacggaaat tgtatgaatt
    13741 gaaaatttta caaagactga aatttcaacg gagagtaaaa aatatccaat taataaagag
    13801 attggatgat gaattaaata aacactcatt ccagaatttc ttaaatggtc gagtatggga
    13861 cctgtatgat ctaagtaacg tttaaaaaaa ggagccaatt caaaaattaa aatccaagca
    13921 aggctactat ctattaacag gtgaacccct cgccaaagta ttcttttgct atcatatgcg
    13981 aaactcatcc aataagggtc tgtttcattt agataaagat aaattagaaa taaaactata
    14041 aaacggatca gtcgaaagat tggttttaat ttagatccaa atgtaatatc aggattttgt
    14101 aaaatttcat ttttataagc atagacacca attagaaaaa aacttaggta gtaaatgaag
    14161 ctaacaggtt gtatccgcaa aaaggaatca tctttattaa atagaaaatt aataagatag
    14221 atcgaagcaa aagaaagaaa cacaaagcgt ataaataaat tgagagaatt ggttttttta
    14281 aattgaatga tcttattcaa aaatgaattt ttgaagagaa ttttcgctaa taattttcct
    14341 atatgaaatt tatgatttaa taaaagtaaa aagctaaata aaaaaagata aactaaaaac
    14401 cagatatgag agagatcaaa ctcattgggt gtaaaaaata agggataata ttccaaaaaa
    14461 gaaatcggat tatttttttg attgtatacg atgaagtatt gaatgggaga aaagaaaagc
    14521 aatccaacta tcatcggagc aaacatcctt tcaaatctgg aaaaagtaaa atttcttcca
    14581 ccatgtttat ttaaaaccat aaaagaaaaa aaaccagata aaaagaaaaa taaaggcatc
    14641 ctaaaaacgt gaataaaatg aacaatgaca gagaaaaaat aatttctttc cggattttta
    14701 attgcatagc ctatttcagg cgcataaaca atcgatgtgt gaaaaagaac gcctagaagc
    14761 aaagcaaaag tctttagatt atctagataa aatagtcgtt tatgcatgac tcattaagta
    14821 gaatctatat ttttccgatg tatagtaggg acattatgaa aaacagattt aaccttttta
    14881 ttgtatttat ttttttttat ttcccactgt ttgcaaaaga agtcgtcatt gttggaaaca
    14941 tcattggtgc cggttcttat attgaaacag atgataatac ctatcctttt aaacatactg
    15001 aattgcaaaa ggaactttct gagctgactg gcaaaaaagt gagaatgctt tgtctctttg
    15061 aggaggattc ttgtgttcca cttcgttatg agattgcacc ttttgcaaac gacaagaatt
    15121 tagcaaagtg gacaattaag aaaattccta aatatgttta tagggggctt atcgcattta
    15181 acccctctgt tactccggat ggaaatattt tattttggac tgtattaact ccagaaggac
    15241 atggaagcaa tactcaaaaa atttggtata gcgagatgga cactcatgga ttctggaaaa
    15301 agggagtgca gatggatcca ccactcaata acaaagctcc ggctgcaatt atctctgctc
    15361 tacctggtgg aaatgaatta tttgtttttg gaagtcatgc agatcaagaa tcattcgaag
    15421 aaataaaaag acagatggat gcggaaaaag cggaaattgt aaagacatct aagtctgcaa
    15481 gagaagtaga aggacgatat gcggcagtga gagaaagata tcgtagagaa atggaaaaaa
    15541 tccaaaacaa agttcctctc tataaaagtt tcaaacaagg aaatggttgg agtgttccgc
    15601 agagaattcc ctttcctgat ttttataatt tgtatagaag cgaagacaat gctaatttac
    15661 aaatctttgg aggatccact ctttcttcaa gtggcaaaac tttgatttat tctgcaaagc
    15721 ataaagattc ggtaggtaaa ctagatttat acgtttcgaa tataacagac ggagttttcc
    15781 caattggaac caacttaggg aaggagatta acacagaata cgaagaaatg gctccctttc
    15841 ttgcgtcaga cgatagaacc ctttattttt caagcaatgg tcatagtggt ctttctattt
    15901 actttacaca aagagttgga gatagttgga ctgaatggtc tcatccacaa gagatttcta
    15961 aaaacttaaa aggtgtaaat ttcttttcga ttccagcttc tggaaattgg gcttatgcga
    16021 gtagagaagg acaattgctc atgacgtatt tacccaatga aacaaagcca aatcctgtga
    16081 ttatcgtaaa gggtagaatt acaaatgaca aaggtgttcc cttaggtgca gaagtttatt
    16141 atgaatctct tactacaaag gaaaacaaag gctctaccat ttcagatcca aatactggaa
    16201 agttttcgat tgttttgcct tatggtgaca attatggatt tcatgcaaag aaagaaggat
    16261 atttaccttt tcatagaagt aaagatttga gagaatcgga aaaactctac caagaagtag
    16321 aagtagatat gattcttcca aagatagaaa aaggtgggga aataacgatt aataatcttt
    16381 tctttgaatc caatcgaagt gaaattaaaa aagagtcaga acctgaacta gatcgcttgg
    16441 gtgagatcat gaagatgaat aaaaatctag aagttgcact tgaaggccat actgataatg
    16501 ttgggaagag tgcggataat attgctttgt cgctcgcccg tgcaaatgca gttgctgaat
    16561 atatagtcaa aaaatttgga atcgaaccaa gccgtttaaa ggtagaagga agaggagaag
    16621 aagcacctct tacagataat aataccgccg atggaagagc taaaaatcgt agagttgtat
    16681 ttcgaatttt aaagaactag tttttttgcg caagtgcgta tatttataaa taaaatgcgc
    16741 acagcctaag ggtttttata cccaatacaa gtatgagatt cagattctta tgcttgtatt
    16801 ttgtttcgaa cttactttta ttctctgaag agctgaatag acagtatgtt ttagatgcat
    16861 atagtttacg aaaagagtcc tttgggcttg gtattcaaaa aaaggagaat gccgatagtc
    16921 tttggacttg gtatcatttt gcaaaagagt ttcgaaatag tttcagattt gctttacaac
    16981 tatcggctat cagccattcg aagaaaaagt tagaagtaga tttcttttcc tcaaagtatc
    17041 aatttccatt ttctagttca gagcagctaa tcacgattag ccctggattt agatattcct
    17101 acattcattt ttatacaaag ttctattcga atacttccgg tagatacgat gcgatattag
    17161 ggctttggct ttacaaagaa aaatcagttc tgttgagtta ttcgaagaat tcaaaggagc
    17221 aaagccattc tttttccttt tccattctaa caggtaagaa tccaacaatt gcattgggtt
    17281 taggaaagga atataaggga ggtaatactg agtggattgt aaatttagga gtaagttttg
    17341 gttttgattc ttatgagacc ggaatctcct atttgcctag aaccgaaaaa gaagattcag
    17401 gaactttcgt atttgcttct aggagtttaa aagaaaagga ggaatccctc tttggagagg
    17461 aaatgaatcc ttatgtggat aatggtcatt tgccagaaaa aagagaacaa gtcgcagaaa
    17521 ttagaattaa aaaaagggaa agagttgtat atgaaatttc gattgatgaa ttattaaaat
    17581 ttagaatccc acttgcaatc gcaattcgaa ttcacagggc gagtaaatct aaagaagact
    17641 acttagagct actaaaaaga ctaccagctg atttagttcg aaagtgtaac aagattcaat
    17701 ttgataaagc aaggaaaaag aattgaatcg gttcttgtat tatttcttgc tttttttatt
    17761 tgtaggtaag atttactcag aagaatctcg ccctaggatt attttctatg gaacaacaga
    17821 atattttgaa agtagatatg taaaagacaa ccaaagaacg aaaaccaatt tttatgttgg
    17881 ttctgtttat aaaaactggc agggtgctgt ggagaagagg ggagaaactc cattagcctc
    17941 gatggaattt aaaaacaaat atttctattt ctccgcgggg aatcgatata aaccaatctc
    18001 cgggttttat atcctaagaa atgaaaaata ctattctgca tttcaaaatc caagacttgg
    18061 tttgatagaa caacctctaa aacgttctta ttggatgggg gtaaatccat ttaactggtc
    18121 tgctggattt tttattggtg aagatatggc tgtgaagaga cccgcttttt attttaaatc
    18181 tccagagaat cttttggctt atacttatag tccagaaaca aagattcatt ttatagctgc
    18241 taatattaga gactatcgtc ccaagagtct caataaaatg gaatttaact taagttctca
    18301 gcttttagga aagagagaaa attattttgg gtatttcaat tttagagtat ttgaaaaaga
    18361 aaaaggttta gagacagagc tttcacttta taaagaagac aatggaggtc tttttgtttc
    18421 aaatcaggat aatttaaaag agacttcttc cgaaaaggca gtctttctta aaatttcaaa
    18481 ataccattac aatcgagtgg agatttttca aacagagaca aaagccctac gcgagcggag
    18541 aatcggtatt aactcttccc tattctcagg gaaatatgga gccatttgtg tatctggtag
    18601 gggctatgag gatgtaaaaa gtctttcaga taaaagagaa atttcgacta ctattggagt
    18661 tggaatttct tatgaatata gattcaaatc tacagaattt atgtttcgtt tggagcaaag
    18721 aaagaaccaa gatgaattag ctgaactcaa atggacaata cgaccaatag tagattggaa
    18781 attggaaatt tcttcgcttt tacaaaaaga ttcaaatcaa tttcgctctc tttatgaaca
    18841 atggagtgat ggagaaaata taaatacaat tcttacaaat cggtctgccg catttaaatt
    18901 aaagcttgtg agtagctttt tagtatttaa cgttagtgga tctagaagac ccaacggaac
    18961 gggagaagtt tacttcgcaa atattcaaat gaaacaggag ttttaagcaa ttcttaggtt
    19021 tacttgctgc tcgagtttcg caagaattaa acttatccta ttttgggaat gatctcggtg
    19081 ttagtgtgcc gactcttgct agttggattt taattttaga attaagttat attatttttt
    19141 actccttact tattttcaaa attttgggaa aagaattatc aagacaccta agatttattt
    19201 tcatgatacc ggtatcattc tacacttatt gaatttaaaa gaaaaagctt tgttagagaa
    19261 agcccttttg tcggttccat ttttaaaaac ttagtcgcta gtgaaatatt aaaaaagaaa
    19321 tttgcagatg aaaagaaacc tgaaatttac tattggatat ctccgagtcg tatcgaagta
    19381 gatttaattt atgaggataa tggtcgttaa attccctgtg aaatataact ctaaaaaatt
    19441 atagaaccag aattcgcaaa aaacctacga ctgtggatga aaatttctgg agttaaatca
    19501 ggtctcattt tatcaaacag caccgaaaag tagtcctatc tggaaatatt caaaatagaa
    19561 atttgtaaaa tctttagttt ttgtatcaaa gcctcgaaag cacaagtgga attcctgctg
    19621 aaaataagat tgcagctagg ttcgcgcacc taaaaattac ttgtatttaa ttggtgtaaa
    19681 accaccaact gttactgaca gaaaataggg agatatcgta tgaatacaga gaacaaagag
    19741 caatcgagtt taaatgaaag tatagacaaa gtaggaactt acttttcaaa acaaaaacaa
    19801 gtgatcaaag aaagtctttt taagggagcc ttccaaggag ttggattcct actagcgata
    19861 ggcgtaacga ctgttcttgc tgtagcagtg acaggaacaa tcaatacttt tagcagtggg
    19921 ggagtgatta agtctgctga tatcaatacg aattttacta cgttaaaaac agcaatcgaa
    19981 ggaataacgt catcacaatg gactacgaat ggggctaata ttggttatac ggcagggaat
    20041 gttggcgttg ggactacgag tccctctgag aaattgagcg ttaaaggcac tggttcgttt
    20101 agtgattcga atacagtcgc atctaatttt cttaacgttg catctgacac tgctgcgata
    20161 aatataatta cacatggaag taattacggt agtactctgt atggcgttag tcgaaataat
    20221 atgggactca ttgaagggca aagtaatagt gcatttgtta taggaactag taatactgca
    20281 ccaattattt ttggcgtgaa tcggactgaa aacatgcgaa ttggaacaaa tggcaatgta
    20341 ggaatcggaa caactagtcc aagtgataat ttctctgttg gaaataaagt tttaatttca
    20401 agttcaagta gcccttcgaa tcctccagca ggtggagcag tattatttat gaatgttggt
    20461 aatgatttaa ttgcgaaaga ctcgacaggg caaaatttaa atataggaga ctttactgct
    20521 tctgataaaa ggttaaagaa agatattatt ccaattattg gggcacttga taaaataaag
    20581 gagctatctg cagtttattt taattgggat cgttcagtaa aaagagtttc cgattattct
    20641 gagaaacgac aattaggtct cattgctcag gaaatagaaa aacagatccc agagatagta
    20701 tctattgatt cgtttgggta taaacgaatg gagtatacta aaatgatccc tgttcttgtt
    20761 tcagcaacgc aagaacaaca aaaggaaatt gaacgtttat cttctaatct tatttctgct
    20821 cgcgaagaaa actctcaact ccgaaaacgt ctcgagaggg ttgaatctgt attagccgca
    20881 gtagaacgtc agtcgaatga gaagatggtt aggaaataaa ttaaagaggt ggaggataag
    20941 caggattcga tgattgattc gattatcctt gtagttgata tccaagaaaa aaatcagtgc
    21001 gcgctataga aaatcgctta gaatttcttt gtgacccatg cctgatgcct tgtattccaa
    21061 atacatcata gactgtaatt cttattctag gaatgcaagg tttattcaat tttgttatac
    21121 ataaaataat tcacaaaagt tttacatttt aaatgaattt atacttgaaa gctctgattc
    21181 gtttcagcac aatattcata ggaaaataaa aatgactttt ttagagattg aaaaggaaat
    21241 aaaaaatttg cattctgggg agaaagccca gctttttcat ttattagcga tggatatttc
    21301 aaaaaatttt ccagggattg aaaagttaca aggagtctgt ggtggtagtg cttgcgtggt
    21361 tcgaactagg attcccattt ggtcattgga atattgccga agaaatggac tttcggaagc
    21421 acaaatactg ttcgattacc cgacgttgcg ggcagcagat cttgtgagtg cttggggata
    21481 tgtaagttta aatcaattag aaattgataa agaaattcag gagaatttgg acgcataatg
    21541 gcaaggtttt attgcaatga aaattttcgc tattctgttg ttttagaatt aagaaaatta
    21601 ggacacgatg tattaactag cttagaagcg ggtaatgcga ataagtctat accagatgaa
    21661 gacgttttaa gtttctctat taatgaaaga cgaatattac taacgtttaa tcgaaaggat
    21721 tttattcgat tgcacaaaga aagatcaact cattttggaa tattagtttg cactgataat
    21781 ccagataata tttttttagc aaataaaata cacgaattag ttctatccca agaaattgta
    21841 aataagctaa ttcgtaataa cagacctaat tcttaaatta aatccgccat agaacgtcag
    21901 gcgaatgaga ggatggtgag gaagtagtta agagaggtgg aggataagta ggattcactt
    21961 gctgagcaac tgattagaga gttttaagaa tcatttctat tttcttatcg gaaatagacg
    22021 ataattctga tttgtcgtat atactcaaag aaatggattt atactcagat ctttttctag
    22081 ggcagtcagc tcttttttta gagagggaaa tttcttaaga agatgtttaa gttctttatg
    22141 gaaattacta tgactaatat ttcaactttc atacagaata gatgagagtg ttggaagctt
    22201 tttatttttt acttttgcgg tttttatttc attcaaagca tctctgattc cagatataat
    22261 ttccatttct ttttgaagag ttttcaattt cttttgcgat tctttccaag tctttattgg
    22321 aacaataaca gcagatttat ttcccttttc gtcagcatat attttaaatt agaaatcata
    22381 taattatgtt aaagttgata tttctatctg tcaagcgaaa agaatacaag cccgtaagtc
    22441 atctttttct aagtcaggat aatcttctaa aatttctttt tggtcatctc tcctgaaagc
    22501 aattctagca ttagttcaac agggtagcgt agatttctga tggtaggctt tccaaaacat
    22561 acttccggat tgagtgtaat gcgtttcaat agattttctg aattcatatc cttggtttac
    22621 cgaattagaa tctattgtca tgtaatttat caaatcgtaa gtcttgcgcg tatcgctttg
    22681 cctagagtgt attgatctgc atactctaga gtgcttccca ctgtaacacc gtgtgcgatt
    22741 cgagtgattt ttaaaccaat cggtttgatt agattgaata aataagaagc agttgcgtct
    22801 ccttctaggg tgggattggt tgcaattaaa acttcttcaa aaggctcttc tttcagacgc
    22861 tctaataatt ctttgatacg aaggttttca ggaccaattc catcgagtgg agaaatgact
    22921 ccatttaaaa catggtattt gcctttgtat tcgcctgtgt tttcgataaa gaaaatatct
    22981 tctggatttt cgatgatgca gataatgttt gtttgcctct tgtcagagga acagatttca
    23041 cagacttcaa attctgtgat cccaccacat tttttgcaga actttaggct ggattttaca
    23101 gtttcgattg tatcgatgaa attttggaaa gaagaagagt ccattctgag gatataaaat
    23161 cctagacgat aggcgctctt cttccctatg cctggtagag aacttaaggc acctaccagt
    23221 tttttaaaaa gagaatcagt caccagatcc accgaagagt ttgcttagct cgttcggatc
    23281 aaatccacca gttacatttt tcatttcatg tgtcatggca tcttttgatt tttttaaagc
    23341 ttcgtttgta gcagagatga ttaggtcttc taacattttc atgtcatctc cctcaaaaag
    23401 atttttatta atcttaacgt tagtaataat gccatctcca ctagcagtca cgtctaccat
    23461 tccagctcct gcagatgcag ttacacggat aacggaaatt cttttctgaa ttgccgccat
    23521 ttggtttttc atgtctttca ttttgctgag tgtttcagcc attttgtcta acttttttcc
    23581 aaacataaat atctccttaa atttctggta atttagatga gtccacttct gttcctaaga
    23641 acttgtcttt taatatagac tcaacgtcaa tttcttcttt ttgtaaaact tctgtatttc
    23701 ctttttgcgg caaattctct tttgccgcta caggatttga ttgcctagga ctcgctgtat
    23761 atctttcgcc ttccttttgc aaaatctggt ataaatcatg aattttctct aaaactcccg
    23821 cgacagaagg tttattcata tcaaagatta attttcttat agaaatttct ataaaaattt
    23881 tcatttcaaa agagtttcgg agtttcagat acattaactt ttcatacata gaatagattt
    23941 tttcagacaa gagtgtcaaa acttctacac tgaaaggctc tagttccttt gaaatttttt
    24001 ctatatcttc aatcgggtag ttgagtgtat ctctatctgc taaattttct ttgatgataa
    24061 tgagtgcatg tgtaaactct acaaattccc aagcaaattt atgtaaatct gtcccttcgt
    24121 tgtagatttt ctcaatcatt tcaaaggatt tatgaaatgt tttttcccct agaatgcttt
    24181 ttaaaaattc gatttgcact tcaacacctt ggtagccaat cattttacca atttgttttc
    24241 cagtgatatg accatctgta aaagtcacag cttgttctaa aaaagaaagt gtgtccctaa
    24301 cagatccatc tccttttttc gcgacccaaa agattccatc agcatcataa tcaatttttt
    24361 caatcttgca aagatgttct acatatgatt gcaggttggg aagaggaact tttttaaaaa
    24421 caaagtcctg gcatctagaa agaattgtct ctggaatctt atgaaattct gttgttgcga
    24481 gtataaatac cacatggtga ggtggctctt cgagtgtttt caaaagtgca ttaaaagact
    24541 ggtctgtgag catatgcact tcatcgatga tataaatttt ataacgtcca cccatgggag
    24601 aaaatttcac attttctcgg agatcacgaa tgttatcgat cccacggtta cttgccgcat
    24661 ctatttccaa cacatcattt gaaatacctt tcataatttc tgtgcaggat ttgcatttat
    24721 tgcaaggctc attgtcctca ggagattcgc aattgagtcg cttggctaga agtcttgcga
    24781 ttgtagtttt tccgacgcct cttggtccaa agaaaatata ggcgtgaccc actctgttgt
    24841 ttttgagtgc attcataagg gcaccaattg ctaagtcttg gtgaatgacc tctttgaata
    24901 gctgagggcg atattttctg gcaaaaacta ggtggctttc ttcggacatg gtagttaaat
    24961 cggagaagcc gggattcgaa cccggggtgc ttttggcaca cacgctttcc aagcgtgcac
    25021 attagaccac tctgacactt ctcctgttgg aaattccaat aaatttgaaa ccctgaataa
    25081 atcgagcgaa aaatatttta ctcttaggag gatggactcc ttgtttcata gtaatagatc
    25141 gttactcaca agtagattaa aatgaactac cacctgctgc gaacaagcgg ctatggattt
    25201 gtgtagaaaa aagatgggta tcgatgtgtt ctaagcaagg aggatagtat gaaaaaaatt
    25261 tacctagcag gtcctgaggt gtttttaccc gattcattcg cagttttgaa tcaacacaaa
    25321 gaaatttgca agcgctacgg ctatttggga ttatccccct ttgatggaga agttactaca
    25381 gaagttggac ttcatcgagc aaagaagatc tatttagaaa attgtaaact cattcgggaa
    25441 gctgatattg ttttagcaaa ttgcaattct tttagaggag cactgatgga tgatggaact
    25501 gcttttgaaa taggttacgc gaaagcactt ggaaagaaaa tctttggcta tataaataaa
    25561 agagaacctt taccaaatct tgtggaaaaa gtagttccga cggttgcgca tccttccggc
    25621 tatcgaatgg atagtcaagg ttatttatta aatgaagact ttggtaactc tattaaccta
    25681 atgcttgaat tttccatttt agaatccggt ggagaactgg tagaaggaag ttttgaagat
    25741 gcattgaaac gaatcgtttg aattcgataa gaatttttcg tgagagcata tgaaaaataa
    25801 agctaggcgg ttactgagcg gagtcgaggt aacccttctt tttttccgat aattcttgta
    25861 tgtaaataac gtttctgtaa acggaaggga actctccctt aatccatcgg aatgggataa
    25921 aaggccaaag aagttgtgct tctaggattg tagtatacct aatttcaaat ttagattctg
    25981 caaaatggat tttgattgta tattccttat tctttaaaaa tgcaatagga aaaggatctg
    26041 attctataaa ctccccataa cgaaagggac gaatgtattc gccgcgaagt tgaatgcttc
    26101 ggttaaatag gtttacaaat aaaaatagaa atcatttaac tctcatttaa aaaaagaatt
    26161 ttgaaaaatt aaatagattc gcgcaaactt tcattatccg ctataagaat ttttattttt
    26221 tcattttgca atatcagatt tgtatagttt actataaatt gttttgcatc aaagtaagaa
    26281 gaggattcta aattgatttt catgaatcct tcgagtagct ccgtgagctt agattggata
    26341 tcctcaagtg cttgtccaaa caaatgatac gattcttcgg attcgtttaa aacgttatag
    26401 taaaaccaac ctaattccat ttttgcatct aggttgttag aatctaattc aattgctttt
    26461 ttgagggaaa gttctgcatc tttcaaatca tattttaaat tttcagataa ctgaatgcaa
    26521 agtcctttta aaatcaatat ttcggatgtc atgaaatttt ctggaatttg ttctatcaat
    26581 gagagagctt cttcgaactt tctaagtttc atttcttctt ttatttgtag aatgatcatt
    26641 cgaatgtcct agaatttttc cttgagtcgt gttgattgct cgacaagttt tttagcctat
    26701 tgtgcagttt cgacctctcc cgttaaagtt caatgatttc ttcaagtgtt ttattttcga
    26761 attcaaaaca ctattgataa aattttttaa aaaacaaaaa ccagttacta gaaaaaaact
    26821 tgtaaataat ttatagaatt ttatggttcc ttttgaaatg aattctgtcg gataatcgcg
    26881 cgaaccttcg gggagtgagg aaagtccgga caccacggaa tgaatgaacc ggataacatc
    26941 cggaatcaat tttccaaaga aaattgagat ggaaagtgcc acagaaaata taccgcctga
    27001 gaaaagtggc aaagttgttt taacagccaa gctacgaatc gggtaagggt gaaatggcga
    27061 gttaagagct caccgcttct tttgtaaaaa agaaggcagg gcaaaccctt catggtgcaa
    27121 tctcaagtaa gcagccgtta tgagtccatt ccaccatggc tgtgggtaga gagcaaagag
    27181 cttcggagta attcgaagac aagataaatg attatcgaat cctttacggc taacgctgag
    27241 aaggattgac agaatccggc ttatagacag aattcatttc ttcttctttt tttcctttgg
    27301 tggattacag ctcttagcct ctccaagttc acaagctttt ttgaaacttt catttgcctc
    27361 atccgatctt ttttctgctt ggagatactt tcctaattca taataggctt ttcccatttc
    27421 tgggtgagat tcgattactg attttaaaac tacaatcgct tcttggttgt ttcctaattt
    27481 agaataagca tttgcaagac catagcttgc ctctatattt gcagaatgaa gtgaaattgc
    27541 ttttttataa agatcaattg cttcttcaaa ttttctctgc ttctttttta agtctgctaa
    27601 tcctttatgt ccgtaagatt tgacttcttc atttccaagt tccactgccc gagtaaattt
    27661 ttcttttcct ttctcatatt cttttttgag aacatatgtg gagccaagtt cattgtagat
    27721 catatattca tttggatcag cttcttgcgc tttgttataa taagaaattg cagactcata
    27781 atcttttttt gtgatatata cttctgctat gcttcgcaga taatagctgt tagatggttc
    27841 gatctcaatg catttttcgt aagatttaat tgccatatca attcgatttt gttctagata
    27901 tagatttgct aaattttcgt aagcaagtgt tagttttggt tctactttgg ttgccttttg
    27961 aaagttgatg attgccatct gaaaattatt tctactctta taaacattac ctaatagatt
    28021 ataaaaagat ccttttttgg gatctagttc aatacacttt tgaagaagtg tagttgcttc
    28081 ttcataattc ttttccctaa gagcgatatt cgctagagct tcataaggaa tcgtgctttt
    28141 gggctgaatc ttttgtgcaa gagttagata ctcgttcgct tttttatatt ccttttttct
    28201 aatataataa ttcgcataat gtatttggac ttgaaatcca tttggttcta tttcgtctgc
    28261 tttgtctaac cattgaagtg cttcttccca tttttcttgg tctgcaaaga cagaactgat
    28321 ctcagcataa atggaaatag ttttgggaga aatctctaaa attttttggt attgttcaat
    28381 tgctttagtg tagtttccag caagtcggta tgcttttgca agatacacat ttgtagaaat
    28441 taaatttgga ttcagtgtat aaatggaaag taagtctttg ttgtcagggg aaagcaactt
    28501 tttattttct ttcgttttta ctacgaagag tctcatagct gcttctggat cttttggatc
    28561 aagttcaact gctttttgtg catgaaattt acttttctcg aaatcaggat ctagataaaa
    28621 ataataaaaa gacattgctc tatgagcgat agacgattga ggagctaaat caattgcttt
    28681 tgtcgctgct tctaaagcaa agactttcat tttttctgca ccgattcggt ctcctgtgaa
    28741 aattctaaat tcataaagac gaagataggt ttctgcgtaa cctatcaggg tgatgaaata
    28801 aaaaattaaa ggaaggaaac aaagggta
//
LOCUS       DAOQOS010000069        28400 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_753_length_28400_cov_6.799365, whole genome shotgun sequence.
ACCESSION   DAOQOS010000069 DAOQOS010000000
VERSION     DAOQOS010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 28400)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28400)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28400
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_753_length_28400_cov_6.799365"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..630
                     /gene="aroC"
                     /locus_tag="PLF66_15230"
     CDS             <1..630
                     /gene="aroC"
                     /locus_tag="PLF66_15230"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001141234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HMZ65447.1"
                     /translation="FANITENLPKTQADVDVNDVRCPNPEAAERMIEKVKEVRKIGDS
                     VGGTIRAVVRNVPPGLGDPVYDKLDGDLGRAILSIPACKGFEIGSGFEGTKYTGSTHN
                     DEFYVEPETGRVRTRTNRSGGMQGGISNGEEILIRAAFKPTATIAKFQNTVNDTPEDV
                     VMKAGGRHDPCVLPRAVPIVEAAINLVLVDAYFYQRALHPDWFLRWKKK"
     gene            complement(671..1777)
                     /locus_tag="PLF66_15235"
     CDS             complement(671..1777)
                     /locus_tag="PLF66_15235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HMZ65448.1"
                     /translation="MATILDITDMASRNVKVAWDLFPSAFSGYLLQDGRRPEIDIKSN
                     HGIHYDWKYGNFGNPEFQKLYVKAKKGQWDADDLSWNTSVDPSNFEFQIFPEKLQPMY
                     GTDMYNKVDRKKQGEMLHSTIAWLLSQFLHGEQGALYAAAMTVEATPWMGAKYYGSTQ
                     VMDEARHVEVFHKYIDTKLNKLYHINDNLFVVIHALTSAGDWDLKFLGMQIMIEGLAL
                     GAFGLIYKFTKEPLLRELLKQVISDESRHVHYGVEALRDYYTKECPEHVRRDREDWAY
                     EVSIMLRNRFMFTEFYEEYFAHAISRDNWIKMVLESNIMSEFRISLFSRLIPNLKAIG
                     LLSDRIKPKYEALGLLRYEGGKSADQISLNELLS"
     gene            complement(1795..2385)
                     /locus_tag="PLF66_15240"
     CDS             complement(1795..2385)
                     /locus_tag="PLF66_15240"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HMZ65449.1"
                     /translation="MPKIVNHAEYREEILEKCFVLFARSGFQGITMREIGKQLDISTG
                     TLYHYFPSKEEIFRQMIIQLSRKQVVILTEQFSKTKSFEERISKLFRFVSQNEEFFQN
                     VLFLIIDYYRQSGLKDPEFFIRDMAAFYRKAIGELVGFGDSPASGAMFTFCLGLVLQS
                     MIQSEGKEREEVFLLIKQMIDSFIASMSAIATQNNN"
     gene            2791..3516
                     /locus_tag="PLF66_15245"
     CDS             2791..3516
                     /locus_tag="PLF66_15245"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HMZ65450.1"
                     /translation="MTLFLDLDNTLYDAKYAYSYAISKLDEEWIRKGRLGNFISLYEK
                     ARQETKADLKNHSSNRLRILYFKKIFDDLEGKLNIDATLELEDLYFKNFIQAIQNYVK
                     ANQANYKTLFNRLKEISNHHKIFILTNENLRTQLMKLGAFFPMNIPIQLLTSEEIGRE
                     KPSPDFFKRALKLSGSKPSECLMVGDNLVDDIKGALGQKIPSVFVKTIFGEMDYSKTK
                     TLSGKKYTEVSNILKALTLFPND"
     gene            3583..4113
                     /locus_tag="PLF66_15250"
     CDS             3583..4113
                     /locus_tag="PLF66_15250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65451.1"
                     /translation="MRWFSLVLFLIFIHCSNSDESDNKKKNESLLVNLLKSKSTTNSY
                     SSSGSGSTSTISSTGGITSSQTNQWQLDYISKLSSGDCKAIASSDKFYCTTADCKGIV
                     TQDKFYCKTSDCKGVANKDKFYCISKLCKAWVTKDKFYCDSSDSDCKAIASNDPFYCR
                     SSQCKAIVRKDKFYCN"
     gene            complement(4150..5106)
                     /locus_tag="PLF66_15255"
     CDS             complement(4150..5106)
                     /locus_tag="PLF66_15255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65452.1"
                     /translation="MVIYINEEVLDTKLEQEKTIADVYDAINQWIESSGKYMVSCIVD
                     GKEKAYSELKDIEIEKVSRLDFYIGEDIDILIASLLELDKYIDNIGNTLFGRDSLTQN
                     ETQNLREGLNWIETVLGSARTLLRLDYTRIIPPGSERNMLEIISKAVDPSNKLDSVPA
                     IEDYLENLRDLKLFIMDLINRVTLLNIDTETVKDILTTYGENMEVLKKEFIRVNENFQ
                     SGKDHLATELLSHSTGRLHIMISGLISVINRDNNLKVEDIKVGEDTLYTVNTEFRDKL
                     SEIEKALADKDIVTAGDILEYELPEILDKFVPFLKEIKTRLN"
     gene            complement(5181..7115)
                     /gene="fliD"
                     /locus_tag="PLF66_15260"
     CDS             complement(5181..7115)
                     /gene="fliD"
                     /locus_tag="PLF66_15260"
                     /inference="COORDINATES: protein motif:HMM:NF018852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HMZ65453.1"
                     /translation="MPAYTVSGLSSGQNTNDIIRKLLELEAKPIKRWEKENEYQKVQI
                     KAWNELRNLTLNLQIKTKALTSFTAPFSSKKVSANEEGYITGEANRNAKSTKQEIEIL
                     KLASKQKIAGNKVKLDTTLPAGQFSIISKDKRIDIDFAGGKLEDLQEKIRNFGSQIVK
                     SNLMKVDSDNMVFSIHALQYGQEAALKFLDPNGILQAAGIVGANVPEPAPTVTNIRLD
                     SAGQTPYQPEKFANSAKPEFAAASSGNGGILLKQNTAFAFPVDKFRVEKLSYLEFYPE
                     KTTGTIPDYLGVGIYYDTKEGEKFKYENIPVKSGKYVVPVAGFARDNLITKIVLGNTG
                     DAEMTVSEIKFVIPGEIQGAAINNIIAPAENALFKIDGIEITRDTNENIKDAIDGVSF
                     TLMKETTQPITMEISVETNKGIVMIKEFVDAYNELIKYSKEVSTSNKDAKVDLSTAAE
                     DNNGVDISAEYWNNKTKSGILAGDNAVVRLIAGLKTATSASYPSSTEPRYKVLSDIGI
                     STGELGSNWKQIQEGLLKIEESTLQIALNDNPESVRELFASDNNNDNRVDDGVGVAIL
                     EHLKPYTQLTSGLVTSKIKLAESTIAENNKKVKEFESHLVNFEKKLKQKYLYMEQGVG
                     KNKAVGNYLKNNFQRKNNDD"
     gene            7198..9255
                     /gene="ppk1"
                     /locus_tag="PLF66_15265"
     CDS             7198..9255
                     /gene="ppk1"
                     /locus_tag="PLF66_15265"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008594414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HMZ65454.1"
                     /translation="MDRTELLNNKDIFFDRELSWLDFNFRVLDEAADTSIPVLERLKF
                     LCITESNLDEFFMVRVAGLRDQVNSGIDDKSLNGMRNSEVILEISKKVKIFIREQYKI
                     LVDQIIPSLKANKIHIITNEADLKPTEISFIKKYYKEEVSSILTPLAIDPSHPFPHLL
                     NRSLNLAITLTGDEDNNRNLFAIVQVPSLLPRFLELPSSNGDRRFFPLEGIITLHLSD
                     LFYGLTVKEIHAFRIMRDSDLSIHEEVEGSLLTNVKKELKNRFWGDAVRLDVHSKIPE
                     TLKHIVMELLKIQDHEMYEIPGFLNMGDLMYFYNLSHTSNLKFSPIPAKNKLGEYSLD
                     KIFQLIRKNDIILHHPYDSFQAVEDLLKFASEDPKVLAIKMTLYRTSGDSPIIQYLKQ
                     AAENGKQVTVLVELKARFDEERNIIWAQRLEDSGVHVVYGIIGLKIHCKVLLIIRRET
                     DKLKRYVHLSTGNYNSTTARFYTDLSLFTNNINITEDIGTLFNALTSSAKMPKLSTIS
                     AAPNYLRDDILGLINQEIENAKQGKPAHIFLKMNSLVDGDLILALYEASSVGIKVDLV
                     IRGICCLRPGVPGVSENIIVRSIVGRFLEHSRIFLFHNAGKPKLFLSSADCMPRNFFK
                     RIEVMFPILDEKVQQKIFKIIEIILKDNVKARILGSDGIYRKLSPVEGEIPIDSQMEL
                     AKI"
     gene            9276..11153
                     /locus_tag="PLF66_15270"
     CDS             9276..11153
                     /locus_tag="PLF66_15270"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HMZ65455.1"
                     /translation="MKHSIIFSFLLFAFTSSYADCEKLLNSFDEPVNLKAGWVFMKGD
                     NPEWKNSDMEDSTWAKKTLPDSGYDPNPDVMVTGYHWYRCHILLPENIKESSISLAVN
                     LGKIRDVDEVFFNGARIGATGKVTPVLQADLEKDRIYSISSRLLEPGKNVLAVRIYTS
                     TDYFGIDSVPILGNEFALQRKNTRKEIFNIISGFVFIAMGLFFIIGSVVRSTNKSNLF
                     FSLFSIILGMYTIIRTQFRYRIFEDFSLSYRVELILLMILPILFVNFITYFVNHKRKL
                     HNWIYEAVMVVLMLLTWFAKTPEKWETIVRYNGYLLLYPLIRCFYIISKNYIQNKSRL
                     KYIFVGTLALLPCVVVDLLKALEFIKFPSIVHFGFMFFLINISIQLSEEMVENYNRFL
                     NQESDLKKMEKSKTKFLVNLSNEFKVYMDGIHEVTKEILSSHETKDTQDKIRRLESYE
                     GLTRSIISDAVVLNAVEGKKYENVIEKFSLKDLVNDTITMIETRLAQKRKSKSVTISS
                     GDVELMQSRELVFLIFYHLLENVYKYTPENSGYQVLIQTDGKTSIEITIADEGNGIDS
                     MEQIDIQKKFVRGSSVNEKIMGVGIGLTLVKAISNYLGGGLEIQSAKGTGSKFIVTIP
                     V"
     gene            11156..13090
                     /locus_tag="PLF66_15275"
     CDS             11156..13090
                     /locus_tag="PLF66_15275"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HMZ65456.1"
                     /translation="MKYFLNIKFVLLILLFFLGNCYAPSEDLITEVLDLSESEWHVDF
                     EDNAEYASPEFSHQSWQAVEVPGNLRLLDSSHRGVFWMRKSFELDAENFTSALALTLG
                     KIYDRDEVYLNGKIIGINGKSPDDIQQNEVAYGRLRIYTIPAGLIRKGTNVVAIKINS
                     NFRAYAGIVSGPVGISTLESASSYLIQHAVDDVIFAAVYLFIGVFFIISFTKMKEMKE
                     YLAFSIFIISFSILHFVRNEFRFEFANNFLVFKYIESALILNMPFFYIQFFQSFFKLS
                     KIKYQNYYFLLNLGLSIVFLAIPNYVFWNNFINIWVINIILILGYSLYVSIQKAKEKD
                     RDAIIYSIALVYFIYSVLKEIFLERGYIQGQSSIEASVLFYILLVTIALRLRFIVLKI
                     KIQNRFEQLKEIDQLREKIFLYMDRILSPSIDESILMTRALKNNNNPEAVAETIPKIQ
                     VIYSEMQNSLDDILELSRLEVMSEPLSKETVDFVDFIRTILAMADVTYTIKVDDKFQI
                     HNSLDLINSLIIRLIDFTGFKDISSIDLIVTSDLKNHLHFRFMLYSVDHKKTQKLYKQ
                     LNDFTVGGNINSVRWAIINEILRLLDGHLEMRLINKKYVRIDFELQALPLEEEKVSKP
                     KFDFKIPKLKFNIKLPTFKK"
     gene            13102..13971
                     /locus_tag="PLF66_15280"
     CDS             13102..13971
                     /locus_tag="PLF66_15280"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HMZ65457.1"
                     /translation="MDDFVIAKDGTRIAYHIRGEGKNTIFLFNGFTCSEPNVKLLVNI
                     LSQKYRIVFWDYKGHGQSETPRNFKEVTVAGSVDDAKRVLDKLEIKEAIFLGYSTGVQ
                     IMFEFNFRYPEYAKALISISGFSDRVMDSFLNLDTTVFGQLGEAMKRLSPVLPHAFSN
                     AWRWIHSLPFDLRLFIASKTFLNEEKTVKEDIQPFLDSMHKQDLNLLIHFIMDVQNHP
                     LSKPLETIKLPFLIMAGGKDLFAPAKRSEEMHRKVSHSELLVISHASHNIIQEEADTL
                     GERILTFLNKNGL"
     gene            14189..16027
                     /locus_tag="PLF66_15285"
     CDS             14189..16027
                     /locus_tag="PLF66_15285"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HMZ65458.1"
                     /translation="MKKLFYILTSFLISFPILAEDALDINFISNNSILLLEYTTVLED
                     KTKSLTMEDILKPENQSRFDDRKVKYSYNFSHTNSAYWLKINLKNLNKEESNIYLEIS
                     YFLLEFVDLYKPAGEKYNVSKSGFSVLQKDREYQSRFFVFPFTIPANSTQSYYLRVAS
                     DNAINLPISLWTAKDFHTHEKNHYAIQTIYLGILISMVLYNLFISFLLRSKDFLLYVI
                     FTLMVGMSFLSISGLLAEAFSNIPPQFIKILPMFFATIGMISFLFFIRRTLHTKTISP
                     KLDKVIIFFIGILFASILPLIIRFKLFARVSILFFLFTSILILSVLILYSLKKVRSAY
                     FFLLAFLFFFLTMILAMLRAVGIIPTNAFTYFGPQLGSVVEMLLFAFALADRYNTFRM
                     EKEKAEKTVKLYLEKSNQELEQKVKQRTKELEEANRELSRMAKQDGLTKIANRRMFDE
                     YLQTEWIKHLKQNECITLILIDIDFFKNYNDYYGHLKGDDCLVQVATAIQDIPKKPSD
                     LVSRYGGEEFAVLLPNTSLSTGLVLAEEIRNRIESLAIPNNNSMVSPYVTLSLGVAAI
                     VPRPRTSPEDLIHIADSALYQAKKLGRNRVIYTKRQKKLPTRILLE"
     gene            complement(16218..17108)
                     /locus_tag="PLF66_15290"
     CDS             complement(16218..17108)
                     /locus_tag="PLF66_15290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65459.1"
                     /translation="MNLLRYSFFLIISLFLIDCASLTFNSKLVFIRSDQIDAEIFVNG
                     KKVGVDSAVIWGSPNKGSLQVEARKEGFEPAKAEIKICLNWITLGNFAYLGASVIGFA
                     IDYFSDSMFYYTESSKVLKLKPKNTFTKQKDEFLLDDITKFAFDSEAKVQVFNMENVI
                     FSESLFLDEVNLSKKTELYPGYYKVNGKVVYEIVRRRKVRGRYQYSYTTYKAPDQVEK
                     VIRIQPNGITIICGEFDKQNKTSSLSAFHVPYEIVKEHNEESWYSPKNPHKASYASNA
                     CSHSGAEDLRDKEYLFDRYK"
     gene            17588..18916
                     /locus_tag="PLF66_15295"
     CDS             17588..18916
                     /locus_tag="PLF66_15295"
                     /inference="COORDINATES: protein motif:HMM:NF019436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65460.1"
                     /translation="MTDLNIPNDSKENIFSKLKSSLKLTEPILNGIFGNYLEESGNEL
                     AIKPGFYYKNKIVDQSSINDLYSELTPNLLIFVHGLACDETYWSFKEESGEKNSFGEI
                     LSSKYNFTSLYFRYNTGRHISDNGNILTDTIEKLVSFYPKKIESINLITHSMGGLVTR
                     SACYYGEEKKQNWLKYINQIFLIGSPHLGAPLEKFGNIVTNVFTSLPQSYMKLAGDVI
                     NQRSSGIKDLRYGSILEEDWKDKEEDALLVNNRTDIPIYENAKYYIVSGSIHKNPKSV
                     LNHWFGDAMVRKKSSRGEGIFDQRDENFFEFPGIDHIQLGHSKEVLSKLEEWLLLENK
                     QLTLTNKKELSNLQEEEKSITKEQMVGLSKLVEKAIAEGAKSVEEVQKELTSKPYDIL
                     SKIFPGIQLTEKIKDMHNTANAGIYKSIQVVNEEINKIARKMLKNDSNTE"
     gene            complement(18963..19781)
                     /locus_tag="PLF66_15300"
     CDS             complement(18963..19781)
                     /locus_tag="PLF66_15300"
                     /inference="COORDINATES: protein motif:HMM:NF025918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042546 - cell wall biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HMZ65461.1"
                     /translation="MLIEKLKYIWCIPKYLPYLQPNLTDEVIKMAESKMGYKLPKRYL
                     ELLSFQNGGYIRYTIPGIPHSQIYGIGPYFPSITDFQWLYEYEGYGLEIRGLFPFDGD
                     GHWNLCLDYRRNKEEPEITFVDIEIISESLIAKNFDEYISKLIIETENEYVIQTSLTI
                     DEVIKFISTAINCDFGKPDLLAHGYPIYRCKHEGNWIWISPNKVKSGFIRLEDPRYEE
                     LKGQMETESLRFTEIDVNAILITFSNREHAVKLTSLLKNCGLIVNELAQFIDYF"
     gene            complement(20364..20639)
                     /locus_tag="PLF66_15305"
     CDS             complement(20364..20639)
                     /locus_tag="PLF66_15305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65462.1"
                     /translation="MKIFYIILGVLLFIFILFLAIFPKIGNHIEAESIEIYKNPKIQT
                     REDIHLSYLETGSFETLECFLFAGGSFLKPQKGSPMAVLYLIHLEIF"
     gene            21546..22454
                     /locus_tag="PLF66_15310"
     CDS             21546..22454
                     /locus_tag="PLF66_15310"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HMZ65463.1"
                     /translation="MKVIALGVYSAFATGTYKEDGSYLPKWQSNFLIEFNTKGKIRDD
                     VFRFVIDFGSDIRHSLAYKGLKVGDIDAWYCSHPHADHIGGVEGIALSTIFNPFWNKK
                     KAEWLKSPDGNLDTVNDRLFDERNIPNECKPSIWAHKSVLDELWQAAAPGLKTLQGVE
                     QVTLDTYFDVVTMKSNEAYTVKDGDRSWKIDTVLSTHVLAGTNLMPSFGLRFRSSDGK
                     NVLFPTDTLWMMPPNMAGFYKRADVIYQDCETGIKSGVHSHIDDIRLADPEVKRKCYL
                     YHYSEEPRVDEGEFKGILRIGDVHEY"
     gene            22652..23527
                     /locus_tag="PLF66_15315"
     CDS             22652..23527
                     /locus_tag="PLF66_15315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002177000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HMZ65464.1"
                     /translation="MQLEEWNKSGQYYNYLHYKIFYKVEGIGIPLLCIHGFPTASYDW
                     SPIWKDLTKSYQVITLDMLGFGFSDKPKDIEYSILKQADIYESLLDSLQIKDYHILTH
                     DYGVSVAQELLARFQDRQKASDNHLNIHSICFLNGGLFPEMHRPRFIQKLLLTPIGFL
                     ISKLFTKKNFEKSFSEVFGKNSKPTQEELDSFWELIRFNDGHLISHKLIHYITERIQQ
                     RDRWVNAILQSKVPIRLINGPADPVSGIHLAEYYKKIVPGADVILLNEDIGHYPQVED
                     PENVLKFYLEFRKNI"
     gene            23558..23914
                     /locus_tag="PLF66_15320"
     CDS             23558..23914
                     /locus_tag="PLF66_15320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cereblon family protein"
                     /protein_id="HMZ65465.1"
                     /translation="MDPIQETLKETNTSAKESKSIVCAHCLFKITSPEFLFFVDGLHV
                     YHFTNPVGNSFQIRLFSKASGCKEEGSPSIEYTWFPNFAWNFALCANCNRHLGWKYHS
                     ELKTFFGLIANQIIEI"
     gene            complement(23911..25197)
                     /locus_tag="PLF66_15325"
     CDS             complement(23911..25197)
                     /locus_tag="PLF66_15325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65466.1"
                     /translation="MRSSLFISFFLFLSGLYSQNLDSVFENATSLNREKRKLEMENTI
                     SSEIKSFQTKKNESVFKKSLEYMQILNFVDEEVQKTFKIILNEYQIKSESFLSQLLET
                     IYTISPIEFQNEMQRIAKETNNPKHFAIAIHYLLRLNPEERKNYSDLLKQKFKNIEKN
                     PILKILSTYLEKSPFEYLSKRPPILDLLRHNYGEDTFVVFSFQRANRDYPGLAIIKKK
                     DGSFLRREDGSIFTISQLAKSMSDLPSYITNGNTPQGIFSIQKIQSVKNDLIGPTPAL
                     ITALPFEIASDVYFQEVRSKSWDMQMYLDILPSSWQSYFPMKEAYYAGEIGRNGIYAH
                     GTTLDSNFYKGSLFFPNSPTRGCLSAKEIWSRKNGKRLYSDQIVLIETVRKLSTSKGY
                     MIVVDLNEESKPVSLDDILMDVLEAEESPMNESNRN"
     gene            complement(25208..25858)
                     /locus_tag="PLF66_15330"
     CDS             complement(25208..25858)
                     /locus_tag="PLF66_15330"
                     /inference="COORDINATES: protein motif:HMM:NF021529.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2259 domain-containing protein"
                     /protein_id="HMZ65467.1"
                     /translation="MKTKWLSILFFPFILFASDSATPHVFGFSKDGSLFAFSESGVQD
                     GSGFPYANGYFIDVEKNVLKEKTIALLFEETDQSYNPDSDAWLKKQIVEEAKKKYGYN
                     LLSLKMETPIKENTFHFQNKKYILKWEPLIQKKYSDKCMGNPELLSIRLVSSNYEKVI
                     QKDKIVPLSRGNGCARNYSFVGIYYFKNSFAAIFSYRYFSFEGDDLRHIAVTWKLD"
     gene            26368..28101
                     /locus_tag="PLF66_15335"
     CDS             26368..28101
                     /locus_tag="PLF66_15335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65468.1"
                     /translation="MKLKQKTKSTKDTLKEDPPFSEKIYLFFAVLISILVFLYWKPIT
                     SGTTLNFDDQVILTPLYNVHSFSDYIELVKDNTIFDLQPIRDLSYILEIKLARFFQIT
                     LHPQAVNLFLWIFSLFILYKIFILEEIPKQISILSLSLISLHPVAVNSIAWASGRKHV
                     LTMLFILAATYCLLQWLKNRKRYSLFLSILFYTLSCLAQPINLGWILWVFYLFKKREK
                     LDRWTSTTNYFIFILFLIGFIIGIANIYYYASERYLDHLGEKFIYEGWDFLGNRILIF
                     GRFFIQLVLPIQPSITSYDPTSPLALIGFILLLSFLYLFYKFKNENLFIWGLFTIIPT
                     IVSNGPSNQHFGWDTYLLTPLAGSGLMLGIFLKIYKLNSSKIYFTFLPILFLYSIQTK
                     ITADAWLNDETLWNYSLKHERTQSSYSGYLKTNLSLRKNISDLWPILNEFKNKYPKHP
                     DIPYIYGRMILEDKQIDIVKKRELFQRENSDNPWFIYYYSAFDASQKNFIDAEKRLLN
                     LFQVNRKNAILNFKDKNTEVLAALYAMCRFGDGKDCKSKIESIQSNIPSRKFSEKDFR
                     DRLSTFHLTYE"
     gene            28108..>28400
                     /locus_tag="PLF66_15340"
     CDS             28108..>28400
                     /locus_tag="PLF66_15340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65469.1"
                     /translation="MNHKRTYYLYMVSIFPLLFIFGTFYFFVHKSLIAFIGFSFSFFI
                     MYVGFNLLGAIFLFKPIENLWKDTQEYSKIELLENTTLSQTKKKAINRIKNLP"
BASE COUNT         9508 a         4793 c         4516 g         9583 t
ORIGIN      
        1 tttgcaaata ttacagaaaa tcttcccaag acacaagctg atgttgatgt aaacgatgtt
       61 cgctgtccaa atccagaagc ggcagagcga atgattgaaa aagtaaaaga agttagaaaa
      121 attggagatt cagttggagg aaccatacga gccgttgtgc gtaacgtccc tccaggacta
      181 ggtgatcctg tatatgataa attagatgga gatctaggtc gtgcaattct ctcaattcca
      241 gcttgtaaag gttttgaaat tggttctgga tttgagggaa ctaaatatac aggctctaca
      301 cacaatgatg aattctatgt agagccagaa acaggaagag ttcgaactcg cacaaataga
      361 tcaggtggta tgcaaggcgg aatctcaaac ggtgaggaaa ttttaattcg agctgctttt
      421 aagcctacag cgactatcgc aaagtttcaa aataccgtaa acgatactcc tgaagatgtt
      481 gtaatgaaag caggtggaag gcatgatcca tgtgttttac ctagagcagt tcctatcgtt
      541 gaagctgcca ttaacctagt gttagttgat gcttattttt atcaaagagc acttcaccct
      601 gactggtttt tgcgctggaa gaaaaaataa aaagcaaacg ttgttcaaaa caacgtttgc
      661 ttttgtgaat ttaagacagt aattcattga gagaaatttg atcagcagat ttaccaccct
      721 cataacgcag aagtcctaac gcttcatatt taggtttgat tcggtcactg agtaatccaa
      781 ttgctttcaa atttggaatc agtctagaaa atagagatat tctaaactca gacatgatat
      841 ttgattctaa aaccatctta atccaattgt ctcttgagat tgcatgtgca aaatattctt
      901 catagaactc tgtaaacata aagcggttac gcagcattat gctcacttca tatgcccaat
      961 cttctctatc tcttctcaca tgctctgggc attcttttgt ataataatca cgaagtgctt
     1021 ccactccata gtgaacatgt ctagattcat cggaaatgac ttgttttagc aattcacgaa
     1081 ggagtggttc ttttgtaaat ttatagatta atccaaaagc tccaagtgcc aatccctcta
     1141 tcatgatttg cattcctaaa aattttaaat cccaatctcc ggcactcgtt aaagcatgaa
     1201 ttacaacaaa taaattatca ttgatatggt ataatttatt taatttagta tcgatgtatt
     1261 tatgaaaaac ctccacatgt cttgcttcat ccattacttg tgtggatcca taatactttg
     1321 ctcccatcca aggtgtagcc tcaacagtca tagcagcagc gtataacgca ccttgctctc
     1381 catgtaaaaa ttgtgataat agccatgcga tagtagaatg aagcatttct ccctgtttct
     1441 ttctatctac tttattatac atatcagttc catacattgg ctgtaatttt tcaggaaaaa
     1501 tttgaaattc aaaattggat gggtctacac ttgtattcca agataagtca tctgcatccc
     1561 attgtccttt cttcgctttc acgtataact tttgaaattc aggatttcca aagtttccat
     1621 atttccaatc ataatgaatt ccatgatttg atttaatatc aatttccggt cttcttccat
     1681 cttgtaataa gtatcccgaa aaagcagatg gaaataggtc ccatgcaacc ttgacgtttc
     1741 tactcgccat atctgttata tctagtattg tagccatact gtttactcct atccttaatt
     1801 attattttgc gttgcgatag ccgacataga cgcaataaat gaatctatca tctgcttgat
     1861 taataaaaat acttcttctc tttctttccc ttctgattga atcatacttt gtaaaaccag
     1921 acctagacaa aaagtgaaca tagccccact agcaggagaa tctccaaacc caaccaactc
     1981 accaattgcc ttacggtaga aagcagccat gtcccgaata aaaaattccg gatccttaag
     2041 tccactttgc cggtagtaat caataatcaa aaaaagcaca ttctgaaaaa actcttcgtt
     2101 ttgtgatacg aatcgaaaga gtttgctgat tctttcttca aatgacttag tcttgctaaa
     2161 ctgttcggtt aagataacca cctgtttgcg actcaactga atgatcatct gtcgaaagat
     2221 ttcttcttta cttggaaagt agtgataaag ggttcccgtt gatatatcta gttgcttgcc
     2281 aatttctcgc attgtaatgc cttgaaagcc agagcgagca aagaggacaa aacacttttc
     2341 tagaatttct tctctgtatt ctgcatgatt tactattttt ggcatctttt tataacatcc
     2401 gttagaaata aacttatttt ataacgatcg ttcggtcaat aaaaaaatta aaaagattcc
     2461 tgcttttttc aaatctcatc tttgaatcca gttcacttcc gtaacaatcc cagaatacta
     2521 gatacaaaaa atctaatttg tattcctttt agaaagcaaa tacaggcaat cctgtttctt
     2581 taagttccga ttcgaacttg gtttcacaac ctagatcgtt ctctgcacga aacgtctatc
     2641 ctctcaaagc attcgaattt tgaatttttg aaaaatagtc cttttcattt ctatagtaaa
     2701 tttgaggtca aactttgatt caaatacaag atatttagaa agtgtaaaaa ttaacttgtc
     2761 atagaaatta ttcaaaagga aaaagaaaac atgactttat ttcttgatct tgataacact
     2821 ctctatgatg caaagtatgc ttacagttat gcaatttcca aattggatga agagtggata
     2881 agaaaaggta ggcttggaaa tttcataagc ttgtatgaaa aagcaagaca agaaacaaaa
     2941 gcagatttaa aaaatcatag ctcgaaccgc ttgagaattc tctatttcaa aaaaattttt
     3001 gatgatctag aagggaaact aaacattgat gcgacacttg aattagagga tttatatttt
     3061 aaaaacttta tccaagcaat tcaaaactat gtaaaagcaa accaagcaaa ctacaaaacg
     3121 ctctttaatc gactgaaaga gatttctaat catcataaaa tttttatact cacaaatgaa
     3181 aatcttagaa ctcaacttat gaaattggga gcttttttcc cgatgaacat tccaattcag
     3241 cttcttacct ctgaagaaat tggtagagaa aaaccaagtc cggatttttt taaaagagca
     3301 ttgaaactct ctggctcaaa accatccgaa tgtcttatgg taggagacaa tttagtagat
     3361 gatattaaag gtgctctcgg acaaaaaatt ccgagcgtgt ttgttaaaac cattttcggt
     3421 gagatggact attctaaaac taaaacgtta agcggtaaaa aatacacaga agttagcaat
     3481 attttaaaag cactcacact gtttcctaac gactagagga ttcccgcatt tagggttact
     3541 acctatttcc ttttttaaat tttaattata aactgaatca ttatgagatg gtttagttta
     3601 gtattgtttt taatattcat tcattgttcc aattcagatg aatctgataa taaaaaaaag
     3661 aacgaatcct tacttgtcaa tttactcaaa tcaaaatcga caacgaatag ttactctagc
     3721 agcggatctg gatctacttc tacaataagt tctacaggtg ggataacttc ttcccaaaca
     3781 aatcaatggc aactagacta tatttcaaaa ctctcctcgg gtgattgcaa ggcaattgca
     3841 tcttctgata aattttactg cacaacagct gattgtaagg gtatcgtcac gcaggataaa
     3901 ttttactgta aaacctctga ctgtaaagga gtagcaaaca aagataaatt ctattgtatc
     3961 tcgaaattat gcaaagcctg ggttacaaaa gataaatttt attgtgattc gagcgactcc
     4021 gattgcaaag caattgcatc aaatgatcca ttctattgta gatcctccca atgcaaagcg
     4081 atcgttcgaa aagacaaatt ctattgtaat taaaattcac agagaatctg aaatctctat
     4141 tcaactgaat taatttaaac gggttttaat ttcctttaga aatggaacaa atttatccaa
     4201 aatttcaggc aattcatatt ctagaatatc tccagcagta acgatatctt tgtctgctaa
     4261 agctttttct atttctgaaa gtttatctct aaactcagta ttcactgtat agagagtatc
     4321 ctcgccaact ttaatatctt ctacttttaa attattatct cgattgatta cagaaataag
     4381 acctgatatc atgatatgaa gtctacctgt ggaatgcgaa agcaattctg ttgccaaatg
     4441 atcttttcca gattggaaat tttcatttac acgaataaat tcctttttta acacttccat
     4501 attttcgcca taagtagtta gaatatcttt tacagtttct gtatcaatat ttaaaagcgt
     4561 tacgcgattg atcaaatcca ttataaagag ttttagatct cgtaaatttt ctaagtagtc
     4621 ctcaatagca ggaacagaat ccaacttatt actcggatcc actgctttag aaataatctc
     4681 aagcatattt ctttctgatc caggagggat aattctagta taatcaagtc gaagaagagt
     4741 tctagcagaa cctaataccg tttcaatcca gttcaaacct tctcttaaat tttgtgtttc
     4801 attttgtgta agagaatctc taccaaataa cgtatttcca atattatcaa tgtatttgtc
     4861 taattctaaa aggctcgcaa ttaaaatatc tatgtcttcc ccaatataaa aatcaagtcg
     4921 actgactttt tcaatctcaa tgtcttttaa ttccgaataa gctttctctt ttccatctac
     4981 aatacaactt accatgtatt tgccgcttga ttcgatccac tgattgattg catcataaac
     5041 gtctgcaatt gttttttctt gctctaattt ggtgtctaaa acttcttcgt ttatataaat
     5101 gaccatgaca ttatctatag aaattgaaag actctatcgg gcaaatacat tttgccaaaa
     5161 ggaatcgatt ctaaggctcc ttaatcgtca ttattttttc tttgaaagtt attctttaaa
     5221 taatttccaa ccgctttatt tttacctaca ccttgctcca tataaagata cttttgtttt
     5281 agtttctttt cgaagttcac aagatgagat tcaaattctt tcactttctt attgttctca
     5341 gcaatggtag attcggctaa tttaatttta ctcgttacta gaccagaagt aagctgagta
     5401 taaggcttca agtgttccag tattgcaaca cctacaccat catctactct attatcatta
     5461 ttattatcag aagcaaacaa ttctcgaaca gactcagggt tgtcgtttaa cgcaatctgt
     5521 aaagtagatt cctctatctt taaaagccct tcctggattt gtttccaatt gcttcccagt
     5581 tcaccggtgg aaattcctat atcagataaa actttgtatc ttggttctgt cgatgatgga
     5641 taagatgcac tcgttgcagt tttaagccct gcaatcaaac gaactacagc attgtcacct
     5701 gctagtattc cagacttagt tttattattc caatactcag cagaaatatc taccccgtta
     5761 ttgtcttcgg ctgctgtgct taaatctact ttggcatctt tatttgatgt gcttacttcc
     5821 tttgagtatt taatcaactc attatacgca tccacaaatt cttttatcat cacaatgcct
     5881 ttatttgttt ctacagagat ttccattgtg attggttgcg tggtttcctt cattaaagta
     5941 aacgatacac cgtctatcgc atctttaata ttctcatttg tatcacgtgt aatttcgata
     6001 ccatctattt taaaaagggc attttctgcc ggagcaatta tattattgat agcagcacct
     6061 tgaatttctc caggaatcac aaatttaatt tcggaaacag tcatttccgc atcaccggta
     6121 tttcctaaaa caattttcgt aattaaatta tctcttgcaa agccagcaac aggaactaca
     6181 tactttcctg acttaactgg aatattctcg tatttgaatt tttcgccttc ttttgtatcg
     6241 taataaattc ctactcccaa ataatcaggg attgttcccg ttgttttttc tggataaaat
     6301 tctaaataag aaagtttttc tactctaaat ttatctactg gaaaagcaaa tgcagtattt
     6361 tgtttgagca aaataccacc atttccacta cttgcagcgg caaattcagg ttttgcgcta
     6421 tttgcaaatt tttcaggctg gtatggagtc tgacctgctg aatctaatcg aatgtttgtt
     6481 actgtgggcg ctggttcggg aacgttagct ccaacgattc cagctgcttg taagattcca
     6541 tttggatcta aaaatttcaa cgctgcctct tgtccatatt gaagtgcatg aatggaaaat
     6601 accatattat cagaatctac tttcatcagg ttggatttaa caatctggct accgaaattt
     6661 cgaatttttt cttgtaagtc ttctagctta ccacctgcaa agtctatatc aatacgtttg
     6721 tctttagaaa taatagaaaa ctgtccagcc ggaagggttg tatccaattt aactttattg
     6781 cctgcaattt tttgtttaga agcaagtttt aggatttcaa tttcttgttt ggtggattta
     6841 gcatttcgat ttgcttcccc tgtaatataa ccctcttcat ttgcagatac ttttttggaa
     6901 gaaaaaggag ctgtaaaaga agttagagcc tttgttttaa tttgtaagtt gagagtaaga
     6961 tttctaagtt cattccaagc cttgatctga actttctggt attcgttctc tttctcccat
     7021 cgtttaattg gctttgcctc taactctaga agtttgcgga ttatgtcatt tgtattttga
     7081 ccggaactaa gcccagaaac tgtgtatgct ggcatttgaa atatcctcct accaaaatat
     7141 ggtcgtcctt agaataaaaa agattactaa aatcgactaa aaaaatacgt tggaaacatg
     7201 gatagaacgg agcttttaaa taacaaggat atattttttg acagagagct ttcttggctt
     7261 gattttaatt ttagagtttt agatgaagca gcggacacat ctatccctgt tctggaaagg
     7321 ctaaagtttc tatgcatcac tgaatcaaat ctggatgaat tttttatggt tcgggttgca
     7381 ggactcagag accaagtcaa ttctggaatc gatgacaaaa gtttaaatgg aatgagaaac
     7441 tcagaagtaa ttttagaaat ttcaaagaaa gtaaaaatat ttattcgaga acaatataaa
     7501 atcttagtag atcaaattat tccatctctc aaagcaaata aaattcacat tattacaaac
     7561 gaagcagatt taaaaccaac cgaaatttct tttataaaaa aatattataa agaggaggtt
     7621 tcgagcatat taaccccgct tgcaatcgat ccttctcatc catttccaca cttactcaat
     7681 cgatctttaa atcttgcaat cacactgacc ggtgatgagg ataacaatcg taatctgttt
     7741 gcaattgttc aagttccttc tctacttcct cgatttttag aattaccatc ttccaatgga
     7801 gatagaagat tttttccact agaggggata atcacacttc acctctctga tttattttat
     7861 ggattaacag ttaaagaaat tcatgctttc agaatcatga gagattctga tctatccatt
     7921 cacgaggaag tagaaggtag tctactcact aacgtcaaga aagaattaaa aaatcgtttt
     7981 tggggagatg cagttcgatt agatgttcat agtaaaattc ctgagacact aaaacatatc
     8041 gtcatggaac tactaaaaat acaagatcat gaaatgtatg aaatcccagg ctttttaaat
     8101 atgggagatc ttatgtattt ttataattta tcccatacaa gtaatttgaa gttttctcct
     8161 atccctgcta aaaataaact aggtgaatat agtttagata aaatctttca gctcattcga
     8221 aaaaacgata taatccttca tcatccctat gattcttttc aagcagttga agacttactg
     8281 aagttcgcta gtgaagaccc aaaagtttta gcaattaaaa tgacattata ccgcacaagc
     8341 ggagattcac ctattattca atatctaaaa caagcagcgg aaaatggcaa acaagtcacc
     8401 gttttagtag aattaaaagc tagattcgat gaagaaagaa atattatctg ggcacaacgc
     8461 ttagaagact caggtgttca tgttgtgtat ggaatcatcg gactaaaaat tcattgtaaa
     8521 gtcttattga ttattcgaag agaaacagac aaactaaaac gttatgtgca cttgagcacg
     8581 ggaaattaca attctacaac agcaagattt tatacagatc tttccctttt tactaacaat
     8641 atcaatatca cggaagatat tgggacattg tttaacgcat tgactagttc agcaaaaatg
     8701 ccaaagctat caacgatttc tgcagcgccc aattatttaa gagacgatat tctaggtctc
     8761 atcaatcaag aaattgaaaa tgcaaaacaa ggaaagcctg cccatatatt cttaaaaatg
     8821 aattctcttg tggatggaga tttgattcta gcactttatg aagcaagtag cgtaggaatt
     8881 aaggtggacc tagtaatcag aggaatttgt tgcttgcgtc caggagttcc tggagtatcc
     8941 gaaaatatta tagttcgttc tattgttggt agattcttag agcattcacg tatattcctt
     9001 ttccataatg caggaaaacc taagttgttt ttatcctcag cagattgtat gcctcgaaat
     9061 ttcttcaaga gaatagaagt aatgttccca attctagatg aaaaagtcca acaaaagatt
     9121 tttaagatta tagagatcat tctaaaagac aacgtaaaag cacgcatttt aggaagcgat
     9181 ggaatctata gaaaactttc tccagtagaa ggcgaaattc cgattgattc acaaatggaa
     9241 ctagcaaaga tatagttttt ggatattaaa tcatgttgaa acactctata attttttcct
     9301 tccttttatt tgcgtttact tctagttatg cggattgtga aaagttactt aactcttttg
     9361 atgaaccagt caatttaaaa gccggttggg tttttatgaa aggagataat cctgaatgga
     9421 aaaactctga catggaagat tccacttggg caaagaagac tttaccagac tcaggatacg
     9481 atccaaatcc agatgtaatg gtgacaggat atcattggta tagatgccat atccttttac
     9541 cggaaaatat taaagagtca tctatctcac ttgctgtgaa tcttggaaaa attagagatg
     9601 tggatgaagt tttcttcaat ggtgcacgaa ttggagcaac tggaaaagta actcctgttt
     9661 tacaagctga cttagaaaaa gacagaatct attcgatttc ttcaagactt ttagaaccag
     9721 gaaaaaatgt attagcagtt cgaatctata cttcaacaga ttattttgga attgattccg
     9781 ttccaatttt aggaaatgaa tttgcactcc aaagaaagaa tactcgtaaa gagattttca
     9841 atattatctc tggctttgtt tttatcgcca tgggattgtt ttttatcata gggtctgtcg
     9901 ttcgttccac aaataaaagt aatcttttct tttcattgtt ttctattatc ttagggatgt
     9961 atacaattat ccgcactcaa ttccgttata gaatctttga agattttagc ctatcgtata
    10021 gagtcgaatt aattctttta atgatactac ctattttatt tgtaaatttt ataacctatt
    10081 ttgtaaatca caagcgtaaa ttacacaatt ggatttatga agctgtaatg gttgttttaa
    10141 tgttactaac ctggtttgca aagacacctg aaaaatggga gacaatcgtt agatacaatg
    10201 gctatctact tctttatcca ctcattcgtt gtttctatat catctctaaa aattacattc
    10261 aaaataaaag tcgtctaaaa tatatttttg taggaacgct tgcattgctc ccctgtgtgg
    10321 ttgtggatct actcaaggct cttgaattca tcaagttccc gtctatcgtt cactttggat
    10381 ttatgttttt ccttataaac atttctatac aactatcaga ggaaatggta gagaattaca
    10441 atcgtttctt aaatcaggaa agtgatctaa agaaaatgga gaaatctaag actaagtttt
    10501 tagtcaattt gtccaatgag tttaaagtct atatggatgg gattcatgaa gtcacaaaag
    10561 aaattctttc ttcgcatgaa acgaaagata ctcaagacaa aatcagaaga ctggaaagtt
    10621 atgaaggact tactcgctct attattagtg atgcagttgt cctaaatgct gttgagggaa
    10681 agaaatacga aaatgtgatc gaaaaattct cgctcaaaga cttggtaaat gatacgatca
    10741 ctatgatcga aactagatta gcacagaaaa gaaaatctaa atcagttaca atctcctccg
    10801 gcgatgtaga acttatgcaa tctagagaat tagttttttt aatattctat catttacttg
    10861 aaaatgttta taaatataca ccagagaatt caggttacca agttttgatt caaacagatg
    10921 gaaagacatc tattgaaata accatagctg atgaaggaaa tggaatagac tccatggagc
    10981 aaatagacat acagaaaaaa tttgttcggg gttctagtgt aaatgaaaaa attatgggtg
    11041 taggtattgg tctaacgctt gtaaaagcaa tctccaacta tctaggcggt gggttagaaa
    11101 ttcaaagtgc aaaaggcact ggttcaaaat ttattgtaac gattccggta taaagatgaa
    11161 atacttttta aatataaaat tcgttttact cattttacta ttttttttag gaaattgcta
    11221 tgctccatca gaagatttga tcactgaagt tctagatctc tctgagtcag aatggcatgt
    11281 cgattttgag gataacgcag agtatgcgtc accagaattt tcccatcaga gctggcaagc
    11341 agtagaagtt cctggaaact tgagactttt agatagttct catcgtgggg tattttggat
    11401 gagaaaatcc tttgagttgg atgcagaaaa ttttacgagt gctctagcat tgacattagg
    11461 aaagatttat gaccgcgatg aagtctatct aaatggtaaa ataattggaa tcaatggaaa
    11521 aagtccagac gatatccagc aaaacgaagt agcctatggc agactgagaa tctatacaat
    11581 tccggcagga ctcattcgta aaggaactaa cgtcgtagca attaaaatca attcaaactt
    11641 tagagcctat gcaggcattg tttccggtcc tgtaggaatt tctactctag agtcagcaag
    11701 ttcatatcta atacagcatg cagttgatga tgtaattttt gccgctgtat atctttttat
    11761 tggagtcttt tttattatta gttttactaa gatgaaagaa atgaaagaat acttggcttt
    11821 tagtattttt atcatctcct tttcaatttt acattttgtt agaaatgaat tcaggtttga
    11881 attcgcaaat aattttctag tatttaagta tattgaatct gctttaattt taaatatgcc
    11941 attcttctat attcaattct ttcaaagttt ctttaagtta tctaaaataa aataccaaaa
    12001 ctattacttc ttattaaatc taggactatc gatagttttt ctcgcaattc caaactatgt
    12061 tttctggaac aacttcatca acatttgggt aatcaatatt attttaatac taggatattc
    12121 gctttatgtt tccattcaaa aagcaaaaga aaaagataga gatgccatca tctatagtat
    12181 cgccttggta tattttattt atagtgtttt aaaagaaata tttttggaga ggggatacat
    12241 ccaaggacaa tcgtctatcg aggcttctgt tttattttat attttgctag taacgattgc
    12301 actgagactt aggtttattg tcctaaaaat aaaaattcaa aatcgctttg aacaattaaa
    12361 agaaatcgat cagttaagag aaaagatttt tctctacatg gatagaattc taagtccttc
    12421 cattgatgaa tcgattctca tgacaagagc tcttaaaaat aataacaacc cagaggcagt
    12481 tgcagagact attccaaaga ttcaagtcat ttacagtgaa atgcaaaatt cattggatga
    12541 tatcttagag ctttctcgtc tggaagtaat gtctgaaccg ttatcaaagg aaacagtaga
    12601 ctttgtagat tttataagaa cgatactcgc catggctgat gtaacttata caatcaaagt
    12661 agatgataag tttcaaattc acaatagcct tgatttaatc aactcgctaa ttattcgatt
    12721 gattgatttt accggattca aggatatcag tagtattgat ctaattgtca catccgatct
    12781 aaaaaatcac cttcactttc gatttatgct atatagtgta gatcataaga aaacacaaaa
    12841 gttatacaaa cagttaaacg attttactgt tggtggaaac attaactctg ttcgttgggc
    12901 aatcatcaat gaaattctaa gactacttga tggtcaccta gagatgagac ttatcaataa
    12961 aaaatatgtg cgaatcgatt ttgaactaca ggcacttcct cttgaagaag aaaaagttag
    13021 taaaccaaaa tttgatttta aaatacctaa gctcaagttt aatatcaaac tcccaacatt
    13081 caaaaaataa ggactaaaaa aatggatgat tttgtcatcg caaaagatgg aacacgcata
    13141 gcctatcata taagggggga agggaaaaat acaattttct tatttaacgg atttacctgc
    13201 agtgaaccta acgttaaatt actagtaaat atactctctc agaagtatag aattgtattt
    13261 tgggattata aaggtcatgg acagtcagaa acgcctagaa atttcaaaga agtaactgtt
    13321 gccggaagcg tagacgatgc aaaaagagtt ttagataaat tagaaatcaa agaagcaatc
    13381 tttcttggtt atagcaccgg tgttcagatt atgtttgagt ttaactttcg ctatcctgaa
    13441 tacgcgaaag cacttattag cattagcggt tttagtgatc gtgttatgga ttccttttta
    13501 aatctggata caactgtatt tggacaattg ggagaggcta tgaagcgact atcgcccgtt
    13561 ttaccgcacg cttttagtaa tgcttggaga tggattcata gtttaccctt tgatcttcgt
    13621 ctttttattg cctctaagac tttcttaaat gaagaaaaaa ctgtcaaaga agacattcag
    13681 ccttttcttg atagtatgca taaacaagat ctgaatctat tgattcattt tattatggat
    13741 gtgcaaaacc atcctttatc aaaacctctt gagacaataa aacttccatt tttgataatg
    13801 gcgggcggta aggatttatt tgcaccagca aaaagatcag aagagatgca tagaaaagta
    13861 tctcattctg aattacttgt tatctctcac gcttctcaca atatcataca ggaagaggca
    13921 gataccttgg gtgaaaggat tttaactttt ctaaataaga atggattata gatttattta
    13981 aacaaattca ttctcaactt ctgaatctaa aagaatctga tcttccaata tcattgtaag
    14041 tctaagattt ttgaaaaatg atatatctca taccgcattg aaaatatttt tgttgattct
    14101 taaatatttc aaattaatta ctgacattac gcaaaattta caatttaagg aataaatgat
    14161 ctaacaattt taggtttgga atatcgattt gaagaaactt ttttatatac ttacatcctt
    14221 tctcatatcg tttcccatat tggctgaaga tgcactagat ataaatttca tttctaataa
    14281 ttcgattctc cttttggagt atacaacggt tttagaagac aaaaccaaaa gtcttacaat
    14341 ggaagatata ttaaaaccag aaaaccagtc aagattcgat gatagaaaag taaaatactc
    14401 ttataacttt tctcatacaa actcagccta ttggctaaaa atcaatttaa aaaacctaaa
    14461 caaagaagag tctaatatat atttagaaat ctcttacttt ttattagaat tcgttgattt
    14521 atataaacct gctggagaaa aatacaatgt aagtaaatct gggttctccg ttctacaaaa
    14581 ggatagagaa tatcaaagta gattttttgt ttttcctttt acaattcctg cgaattcaac
    14641 acaaagctat tatctgagag ttgcctctga taatgcgatt aatttgccca ttagtctctg
    14701 gactgccaag gattttcata ctcacgaaaa aaatcattat gctattcaaa caatctattt
    14761 aggcattcta atctctatgg ttttgtataa tttatttata tcctttctac ttagaagtaa
    14821 agattttctc ctttatgtaa tttttacttt aatggttgga atgagttttc tatcaatcag
    14881 tggtttatta gcagaggctt tctctaatat accaccccaa tttataaaaa tacttcctat
    14941 gttctttgca acgataggaa tgatttcctt tttatttttt attagaagaa ctcttcacac
    15001 taaaaccatt tctcctaaat tagataaagt cataatattc tttattggga ttctattcgc
    15061 atcgattcta cctcttatta taagatttaa attatttgca agagtttcga ttctattctt
    15121 tctctttaca tcgattttaa ttctttcggt tttaatttta tactccttaa aaaaagtcag
    15181 aagtgcttat ttctttctct tagcattttt attctttttt ttaacaatga tcctggctat
    15241 gcttagagca gttggaatca ttcctactaa cgctttcact tattttggac cacaactagg
    15301 ttccgttgtc gaaatgcttc ttttcgcatt tgcactcgca gatagataca atacattcag
    15361 aatggaaaaa gaaaaagcag aaaaaaccgt taaactatat ctagaaaaat caaatcaaga
    15421 attagaacag aaagtaaaac aaaggacaaa agaactagaa gaagcaaatc gagagttgtc
    15481 gcgaatggca aagcaagatg gtcttacgaa aattgcaaat cgtagaatgt ttgatgagta
    15541 tttacaaact gaatggatca aacatttaaa gcaaaatgaa tgtataactc tgattctaat
    15601 agatattgat ttctttaaga attacaatga ttattacggt catctaaaag gagatgattg
    15661 cttggttcag gttgcaacag cgattcaaga tattccaaag aaaccgtctg atttagtttc
    15721 tcggtatggt ggagaagaat ttgctgtatt actcccaaat acaagtttat caacgggact
    15781 tgttcttgca gaggaaatta ggaatcgaat tgaatcactt gccattccaa ataataattc
    15841 catggtcagc ccgtatgtca ctttaagtct aggcgttgct gcgatagttc cgagacctag
    15901 aacttctcct gaagatttaa ttcacattgc agattctgct ttataccaag caaaaaaact
    15961 tggaaggaac agagtgatat ataccaaacg ccaaaagaaa cttcccacaa ggattttatt
    16021 ggagtaacgt ttggtataga ccgaacactt atttgtaatt gaaaaatggg aaattaattt
    16081 tggcgttcgg tataggttca gttcagattc tactagcggt tactcatgag tagtctcatc
    16141 tataatattt tttatcgaat caaataagtt taaggatatt ctgttcagtt ccaaagagag
    16201 acttaaatca ataaggatta tttatatcta tcaaacaaat attctttatc tctaagatct
    16261 tctgctccag aatgagaaca tgcattgcta gcataggagg ctttatgtgg attcttaggc
    16321 gaataccaac tttcttcatt gtgttctttg acaatctcat acggaacatg gaaagcagaa
    16381 agcgaactag ttttattttg cttatcaaat tcaccacaga ttatcgttat tccgtttggc
    16441 tgaattcgaa tgactttctc tacttgatct ggagccttgt aagtggtgta actgtattga
    16501 taacgtcccc ggactttcct cctacgcacg atttcataga caacttttcc atttacctta
    16561 taatatcctg gatacaattc agttttctta ctcaaattca cttcatccaa aaaaagcgat
    16621 tctgagaaaa taacgttctc catattaaaa acctgaacct ttgcttccga atcaaaagca
    16681 aacttagtta tatcatctag gaggaattca tccttttgct tagtaaaagt atttttaggt
    16741 ttaagtttta atactttgct cgattctgta taataaaaca tactatcaga aaaataatcg
    16801 attgcaaaac caattacgga agctcccaag tatgcaaaat ttccgagagt gatccaattc
    16861 aaacatattt taatttctgc tttggctggt tcaaagcctt cttttctagc ttctacctgt
    16921 aaggagcctt tattcggact tccccaaatg actgcagaat ctactcctac tttttttcca
    16981 tttacgaaaa tttccgcgtc tatttgatcg gaacgaataa agactaattt gctgttaaaa
    17041 gttaaagaag cgcaatcaat caagaataac gaaattatga gaaagaagga ataccgtaac
    17101 aaattcattt ttttctatac cttttgtaaa aaatctttag tcactcacct aactcaagtg
    17161 cagcaaatat gcaaaatgca gttcactacc aaaaatatta catttgtgta atttcgtatc
    17221 attttatcct ttctagatgc caaatcattg taaaaagaaa tttctaaaat gcgttagtga
    17281 cttgaatatc cgactatgat tagcagaaag tataagcact tctttagaaa ataattctcg
    17341 agtcagtcac tcattaaaac ttagactata tttcccttca accagtcaac tcggtcaagt
    17401 aaaaagattt tttgaaatta acaccgcgac acaaagaaga acagcgaaat taagtttccg
    17461 ccccacttcg attttaaatc ctaacctgat acattgtgtt ctagatatcc tttgtagttt
    17521 tctttgtttt ttttctttta gaatatttta taatttaaaa attagaaatc ttttttatga
    17581 ttacaaaatg actgatttaa acattccaaa cgattcaaaa gaaaacattt ttagtaaact
    17641 aaaaagttcc ttaaaactaa ccgagccaat actcaacggg atttttggaa actatctaga
    17701 agaatcagga aatgaactgg caatcaaacc tggtttctat tataaaaata agatcgtaga
    17761 tcagtctagt atcaatgatt tgtattcaga acttacaccg aatcttttaa tttttgtgca
    17821 tggtttagct tgtgacgaaa cttattggtc ttttaaggaa gaatcaggag agaaaaatag
    17881 ttttggagaa atcctttctt ccaaatacaa ttttacctct ttatattttc gctacaacac
    17941 aggtagacat atttcggata acggtaatat tcttacagat acaatcgaaa aattggtctc
    18001 cttctatcct aaaaaaatcg aaagcataaa tcttatcaca cacagtatgg gaggactcgt
    18061 taccagaagt gcctgttatt atggggaaga aaaaaaacag aactggttaa aatatataaa
    18121 ccaaatattt ctaatcggat ctcctcattt gggagcacca ctcgaaaagt ttggaaatat
    18181 tgttacaaat gtttttactt ccttacctca atcttatatg aaattggcag gagatgtaat
    18241 caaccagaga agcagtggaa tcaaagattt acgatatgga agtattttag aagaggattg
    18301 gaaagacaaa gaagaagatg cgttactcgt aaacaatcga actgatattc cgatttatga
    18361 aaatgcaaaa tattatatcg tttccggttc tattcataaa aatcctaaaa gtgttttgaa
    18421 tcattggttt ggtgatgcaa tggttcgaaa aaaaagttct agaggagaag gtatttttga
    18481 tcaaagagac gaaaattttt ttgaatttcc tgggattgat catattcagt taggtcattc
    18541 taaggaagtg ttgagcaaat tagaagaatg gctattgcta gaaaataagc aactaacgct
    18601 cactaacaag aaagaacttt ctaatttgca agaagaagag aagtcaatca ccaaagaaca
    18661 aatggttggt ttatctaaat tagttgagaa ggcaattgca gagggagcaa aatcagtaga
    18721 agaagttcaa aaagaattaa cttccaaacc ctatgatatc ctttccaaaa ttttccctgg
    18781 aattcaattg actgaaaaaa tcaaagatat gcacaataca gcaaatgcag gtatctataa
    18841 atccattcaa gtagtaaatg aagaaataaa taagatcgca cgaaagatgt tgaagaatga
    18901 tagtaataca gaatgaaatc tttttctttt tatatttgcg gttaacaaaa agtagctaca
    18961 ctttaaaaat aatctataaa ttgcgctaat tcatttacga ttaacccgca attttttaaa
    19021 agactagtta acttaactgc atgctccctg ttcgaaaaag ttataaggat cgcattcaca
    19081 tcgatctcag taaaacgcaa tgattcagtt tccatctgtc cttttagctc ttcatatctc
    19141 ggatcctcaa gcctaatgaa tccactcttt actttattcg ggctaatcca tatccagttg
    19201 ccttcatgtt tgcatcgata gatgggatag ccgtgtgcga gtaaatctgg ttttccaaaa
    19261 tcacagttga ttgcagtcga aataaacttg atgacttcat caatcgttaa gctcgtctga
    19321 ataacatatt cattctctgt ttctatgatt aatttactga tgtattcatc aaagttttta
    19381 gcgataaggc tttcagaaat aatctcaata tcaacgaaag ttatttcagg ctcttcttta
    19441 ttcctacgat agtccaaaca gagattccaa tgcccatctc catcgaatgg gaacaatcca
    19501 cgaatttcca agccgtatcc ctcgtattca tacaaccact gaaagtctgt tatagatggg
    19561 aaataaggac ctattccata aatttgagaa tgtgggatgc cgggtatcgt atatcgtata
    19621 tatcctccat tctgaaacga taaaagctct aaataccttt tgggtaattt atagcccatt
    19681 ttactttcag ccatttttat tacctcatct gtcaagttag gttgaagata gggtagatat
    19741 tttggaatac accatatata tttcagtttt tcgattagca tctttatcta tttaatttat
    19801 cggttatcgt atattttcaa ttacaattta ctggtatatt cttcagagta attacggcta
    19861 aataaaagat agccgcactt tataaataaa aattacttct actctaattt ttcaatgcca
    19921 tcatatgcat ttgaatcatg ggagggaact acttttattt caagctttag tttttgaaat
    19981 tctgaaattt tagatatttc atttgccatt gtatttcgat ctaaatctac caataaacta
    20041 ctagaaatat ttatgagttt taaactgaaa ttcttctcac ctccatgtta agtcacctgt
    20101 aaaaaaaaat cttttttcag gcgaaaggtt tacaaaaata ccaatatctt cctttgttgt
    20161 atggaagata acgtccttta gatcctttag tccgctagca tgatcccagt gcatatggga
    20221 aagtaagata ctttcgattt ttattttttt actaaaaaga atctcactta ccgggatttt
    20281 tttctcgtat gtaaaaaaag gttttaacca agtaggcgta gattcatatt gggaatcaat
    20341 attttttcct atacctgtat caattaaaat atttccaaat ggatgagata tagaaccgcc
    20401 ataggggaac ccttttgcgg ttttaaaaaa cttccgcctg caaatagaaa acactctaaa
    20461 gtttcaaacg aacctgtttc taaataggat agatgaatat cttctctagt ttgtattttt
    20521 gggtttttgt aaatttcgat tgattctgct tcaatatgat ttccaatctt tgggaaaata
    20581 gcaaggaata gaataaatat gaaaagaaga actccgagga taatataaaa tattttcaag
    20641 ttagtgtcct atgtataatt ctagcggcta tcgcctactt tttattttaa tgtataagct
    20701 tcaacttatt ttatagaata aattagatct ctttaacaag atatactttc aactcttctt
    20761 tgaccagtat ctaaaatagt tgatattcct aaccgattgg ataattacac tgatggcttc
    20821 tacagtaaaa aatattactt taaatcgata ttttactttt atagaaaata gattcaggaa
    20881 taaatttttc ttccgtcagg aaagaaaaag gcattgaatt tgattttatt tagagatatt
    20941 ctaaagacat aggatgtgga tatgaaactc tttacttttc aatcaattct gatagcaatt
    21001 ggggtaatgc ttgttatcca ttgatagagg ctacaatgat tccctatttt attacaaaaa
    21061 tcgaagctac catcattctt attataataa atacccttac tcctattaca aaggatattt
    21121 gcataagcac tatagattcg ttggaggctt tcaaaatacc tcttttcact ctgggcatta
    21181 tggtaggcat aatagaagaa attagggttc aagagacaaa tgattgtata tcgctttctc
    21241 tctatccttt agactaatcg agagtctatt caatgttagg ttattaaata ggtattatac
    21301 taattatatc actaggattc tagcattatt attacttaga aaaataaaaa tatttttagt
    21361 ttgtattttt ttttctttta catttcctaa ttaatgaaaa tttaattaag aataaaaatt
    21421 caacccaaaa gattatttaa aatgtggatt accttgcttg gaaatatttt ctaaaatgaa
    21481 atttttttct attagtattc ttttttttgt aagttcaaaa gtctaggaat gaatagaggt
    21541 tacacatgaa agtaattgca cttggagtat acagcgcgtt tgcaactggg acatacaagg
    21601 aagatggaag ttaccttccc aaatggcagt caaattttct aatcgagttt aacacaaaag
    21661 gtaaaattcg agatgatgtt tttagatttg taatagattt tggttcggac attcgacatt
    21721 cactcgcata caaaggcttg aaagttggtg atatagatgc ttggtattgc agccatcccc
    21781 atgcggatca tattggagga gtagaaggaa tcgctctttc tactattttt aatccatttt
    21841 ggaataagaa aaaggcagag tggctcaaaa gtccagatgg aaatttggat actgtaaatg
    21901 atcgattatt tgatgaaaga aatattccaa atgaatgcaa gccttctatt tgggcacata
    21961 aatctgtatt agatgaactt tggcaagcag cagcgccggg tctaaaaacc ctgcagggag
    22021 ttgagcaagt aacgttagac acatactttg acgttgtgac gatgaaaagc aatgaagctt
    22081 acacggtaaa agacggagac agatcttgga aaattgatac agttctcagc actcatgttc
    22141 tggcaggaac aaatcttatg ccttcttttg gattgcgttt cagaagctcc gacgggaaaa
    22201 atgttttatt ccctacagat actttatgga tgatgcctcc gaatatggca ggattctata
    22261 aaagagcaga cgttatttac caagactgtg aaacaggaat taaatcagga gtccactctc
    22321 acattgacga tatacgactt gctgatccag aagttaaaag gaaatgctat ttgtatcatt
    22381 attcggaaga accaagagtg gatgaaggtg aattcaaagg aatactccga attggagatg
    22441 tgcacgagta ttaaatgaag tcagttcgat gtaaaagaaa aattaaaaga ttaccaccga
    22501 taaaagatat gataattcaa agttatcaat catgtcttca tcgttccgct atcggcgtgc
    22561 acatagtcga cgtggctcag tgcaagtcgg tggtgaaaat atttaatgtc gaattcacgc
    22621 taagtaattc tactgactcc ttggagatac aatgcaacta gaagaatgga ataaatctgg
    22681 gcagtattat aattatttac attataaaat tttttacaaa gttgaaggga ttggaattcc
    22741 acttctctgc attcatggat ttcctactgc ctcttacgac tggtcaccaa tttggaaaga
    22801 tttaacgaaa tcctatcaag tcattacact cgacatgtta ggtttcggtt tttctgataa
    22861 acccaaagac attgaatatt ccattttgaa acaagctgat atttatgaat cccttctaga
    22921 ctcactacag ataaaagact atcacatact aacgcatgat tatggtgtaa gtgttgcaca
    22981 agaactttta gctcgctttc aagatagaca aaaggcatct gacaatcatt taaacatcca
    23041 ttccatttgt ttcttgaatg gtggtctttt tccggaaatg catagaccta gattcatcca
    23101 aaaacttctt ttaactccaa ttggattttt aatctcaaaa ctttttacga aaaagaactt
    23161 tgaaaaaagt ttttctgaag tttttggtaa aaattccaaa ccaacacaag aagagttaga
    23221 tagtttctgg gaactcattc gttttaacga tggacatctc atttctcata aactaattca
    23281 ctacataact gaaagaattc agcaaagaga tagatgggtc aacgcaatcc ttcaatctaa
    23341 agttccaatc cgtttaatca acggtccagc cgatcctgtt tccggcattc atctagccga
    23401 atattataaa aagatagttc caggagcaga tgtaatcctc ttaaatgagg atatcgggca
    23461 ttatccgcaa gtggaagatc ctgaaaatgt tttaaaattc tatctggaat tcagaaagaa
    23521 tatttaattt gacattcgca tataaaataa ttactctatg gacccaattc aggaaacgtt
    23581 aaaagaaaca aataccagtg caaaggaatc aaaaagtatt gtttgtgctc attgcttatt
    23641 caaaattaca tcgccagaat ttttattttt cgtagatggt ttacatgttt atcatttcac
    23701 aaatccggta gggaattctt ttcaaattag acttttttca aaggcaagtg gttgcaaaga
    23761 agaaggaagt ccgtccattg agtatacttg gtttcctaat tttgcctgga actttgctct
    23821 ttgcgcaaac tgtaatcgcc atttaggatg gaaatatcat tccgaattga aaactttttt
    23881 tggactcatt gcaaatcaaa ttatagaaat ttaatttcga ttcgattcat tcataggact
    23941 ttcttctgct tctaaaacat ccataagaat atcgtctaac gaaaccggtt tactctcttc
    24001 atttaaatca actacaatca tatatccctt actagtagag agtttacgaa ctgtttcaat
    24061 caaaacgatt tgatcactgt ataatcgctt tccatttttg cgagaccaga tttcttttgc
    24121 agagagacaa cctcttgttg gagagtttgg aaaaaataga gaacccttat aaaaatttga
    24181 gtccagtgtt gttccatgtg cataaatacc atttcgtcca atctcacctg catagtatgc
    24241 ttccttcatt ggaaaataag actgccaact agatgggaga atatctagat acatttgcat
    24301 atcccaagac tttgatctaa cctcttgaaa gtatacgtca gacgctattt caaatggaag
    24361 agctgttatc agagcaggag ttggtccaat caaatcattt ttaactgatt gaattttttg
    24421 aatcgaaaat attccctgtg gagtattccc attggtaata taagatggaa ggtcggacat
    24481 agatttggct aattgtgaaa tagtaaaaat agatccatct tctctcctaa gaaaacttcc
    24541 atcctttttc ttgataatgg caagtcccgg ataatctcga ttagcccttt gaaagctaaa
    24601 tacaacaaag gtatcctctc cataattatg gcgcaataaa tctagtatag gtgggcgttt
    24661 gcttaaatat tcgaaaggag atttttccaa ataagtggag agtattttta agattggatt
    24721 tttctctata tttttaaact tttgcttcaa taaatcagaa tagttttttc tctcctccgg
    24781 atttaatctt agaagataat gaatagcaat ggcaaaatgc tttggattat tagtttcttt
    24841 agcaattctt tgcatttcat tttgaaattc gataggcgat atagtataga tagtttctaa
    24901 aagttgagat agaaaagatt cggactttat ttgatattca tttaaaatga tcttaaatgt
    24961 tttttgaacc tcttcatcca cgaaatttag aatttgcata tactctaaac tttttttaaa
    25021 tacagattca ttttttttag tttgaaatga ttttatctca gaggagattg tattttccat
    25081 ctcaagtttt cttttctctc gattcaaact agttgcattt tcaaaaacag aatctagatt
    25141 ttgagaatat aggccagata aaaaaagaaa aaaagaaatg aacagagagc ttctcataaa
    25201 aacctcttta gtccaatttc caagtaactg caatatgcct tagatcatct ccctcaaatg
    25261 agaaatatct ataagaaaaa atagcagcaa aagaattctt aaaataatag attcccacaa
    25321 atgaataatt tctagcacat ccatttcccc tactcaaagg aactatttta tctttctgaa
    25381 tcaccttttc ataattactt gaaacaagcc tgatagagag taattctgga ttccccatac
    25441 atttatctga atattttttt tgtatcaaag gctcccattt taaaatgtat tttttatttt
    25501 gaaaatgaaa tgtattttct ttgattggcg tttccatttt caaggaaagt aagttataac
    25561 catatttctt tttggcttcc tctacaattt gtttttttaa ccatgcatcg gaatctggat
    25621 tataagattg atccgtttct tcgaatagga gtgctatcgt tttttctttc aaaacattct
    25681 tttcaacatc tataaaatag ccatttgcat acggaaatcc agaaccatct tgcactccag
    25741 attcactaaa agcaaagaga ctaccatcct tactaaatcc aaaaacatga ggtgtagcag
    25801 aatcagatgc aaataagata aatggaaaaa ataaaataga gagccatttt gttttcatca
    25861 gatttaaaaa taaaaaaata catcacttat gcaattagaa aatgacttga gcttatataa
    25921 aaaaaattga tgattcaaaa tagattctaa ctcaattaga atagatattg aattgaatca
    25981 aaagataaag aacttgaaag aaatttcaat ctgaatccag tgtttgcaaa cagattcaat
    26041 cgattgctaa ttatttatag aagaattcta ttggatagat cattcatgct attcgttaat
    26101 ttagaaatac cttcctcttt tatttcctat attcgtatct gatccagtga accgtttaac
    26161 gaaagtttgc cgcactacct ttttttcttt ggatctagac ggctacttat ctcaactcaa
    26221 ccactcattg gatctttatc agaatcgttt tttttgatta gtttgagcga ttcctaaaat
    26281 cgacaggcaa aatattaaaa aagagtaatt tcaaaaaacg attgctgcat agaggaaaca
    26341 actaaaaagg tatatatggt gaaaggtttg aaattaaaac aaaaaactaa atctactaaa
    26401 gataccttga aagaagatcc gccattttca gaaaaaatat atttgttttt cgctgtattg
    26461 atttcgattt tagtatttct ctattggaaa ccaatcacca gtggaactac tcttaatttt
    26521 gatgatcaag tgatactaac acctctctac aatgttcatt ctttttctga ttatattgaa
    26581 ttagtaaaag acaatactat ttttgatttg cagccaattc gcgatttgag ttatattctt
    26641 gagattaaat tagcaagatt ctttcaaatc actttacatc ctcaagcagt aaatttattc
    26701 ttatggattt tctctctttt tattttatat aaaattttta ttttagaaga aataccaaaa
    26761 caaatatcaa ttctatcctt atccttaatt tcccttcacc ctgttgcagt aaattcaatt
    26821 gcttgggcat ccggtcgaaa gcatgtttta accatgctat ttattctagc ggctacttat
    26881 tgtttgctgc aatggttaaa gaatagaaaa agatattccc tttttttatc aattttattc
    26941 tatacactct cctgtctagc tcagccaatt aacttaggtt ggatcctttg ggttttttat
    27001 ttatttaaaa aaagagaaaa actggataga tggacatcaa ccactaacta ttttattttt
    27061 attttattcc taattggatt tatcattggt attgcaaata tttactacta tgcaagtgaa
    27121 cgttatcttg atcacctagg agaaaaattt atttatgagg gatgggattt tttagggaat
    27181 cgtatattaa ttttcggtag attttttata caattagttt taccaattca accttcaata
    27241 acctcttatg acccaacttc tcctttggct ttgataggct ttattttact tctatccttt
    27301 ctttatttgt tttataaatt caaaaatgaa aacttgttca tttggggact attcacaatt
    27361 ataccaacta tcgtttcgaa tggtccttcc aaccaacact ttggatggga cacctatcta
    27421 ctaactcctt tagctggatc tggattaatg ttaggcattt ttttaaaaat atataaatta
    27481 aatagttcta aaatatattt tacttttctt ccaattcttt ttttgtattc tattcagaca
    27541 aagataacgg ctgacgcatg gttaaatgat gaaactcttt ggaattattc cttaaaacat
    27601 gaaagaaccc aatcttctta ttctggttat ttaaaaacta atctaagtct tagaaaaaat
    27661 atttcagacc tttggcctat tttaaatgaa tttaaaaata aatacccaaa acatcctgac
    27721 attccgtata tttatgggag aatgatttta gaggataaac agattgatat tgttaaaaaa
    27781 agagaattgt ttcagagaga aaatagtgat aatccctggt ttatctacta ttatagtgct
    27841 tttgatgcat cccaaaagaa ttttatagat gcagagaaga gacttttaaa tttattccag
    27901 gtaaatagaa aaaatgcgat tctaaatttt aaagataaaa atacagaagt attagctgcg
    27961 ttatatgcaa tgtgtagatt tggggatggc aaagattgta aaagtaaaat tgaatccatt
    28021 cagtcaaata ttccctccag aaaattttct gagaaagatt tcagagatag actatctaca
    28081 tttcacctaa catacgaatg aggtattatg aaccataaaa gaacatacta tttatacatg
    28141 gtaagcatat ttccattatt atttattttt ggaacattct attttttcgt tcataaatca
    28201 ttgattgctt ttattggatt cagtttttcc ttttttataa tgtatgttgg gttcaatctt
    28261 ctaggagcta ttttcttatt taaaccgatt gaaaatcttt ggaaagatac ccaagagtat
    28321 tcaaaaattg aattattaga aaatacaact ttatctcaaa caaagaaaaa ggctataaat
    28381 aggattaaaa acttaccaat
//
LOCUS       DAOQOS010000070        28148 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_764_length_28148_cov_6.205354, whole genome shotgun sequence.
ACCESSION   DAOQOS010000070 DAOQOS010000000
VERSION     DAOQOS010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 28148)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28148)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28148
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_764_length_28148_cov_6.205354"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2245
                     /locus_tag="PLF66_15345"
     CDS             <1..2245
                     /locus_tag="PLF66_15345"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HMZ65470.1"
                     /translation="KIGFAFYFVGSVSLLIVGLTSYFVAKNSLKEEAFKRVTLVREMK
                     ASQIEDYFQIIRHETQTFAENKAIVDAMREFKIAFDSIERESYISIKLDTAKENLRSY
                     YDREVITRLKKSAIDINSNSIFPKTNSEVILQDLYISSNTNEVGKKNLLNAARESLSY
                     NTVHAKYHQNFMAIQQRFGFYDMFLVDVDSGNIIYTVFKEVDFGTSLLDGPYSNTNIG
                     RVFKETKNLGQKGYVKLVDFEPYIPSYGAAAAFIASPIYDGNKMTGVLIFQLPMDKIM
                     DVMNFHQEWEKSGMGKTGETFLVGQDLKMKSISRFLVETPEEYFKSLDKSDMPLDVIQ
                     KIKNLKTNVGLQENKTKAGLDIARGISGNEIYLDYRHVNVISSYKPLQIEDVKWGIVS
                     KIDEEEAFGAVSLLKTNLFLISFFVFVLIGFFTLLLSKVIIKPIAAIVHRIEDIANGE
                     GDLTKRLTVTSKDELGDLADWFNKFLVKIHSIVLEIASTSQIILVSSNRLTDTSLSLS
                     SSTEETSVQSELIASASTEMSQSVQVIASSIEEMSISIAEVARRASEYAAISREANKT
                     AIETNAIVNRLGEDAKDIGKVIESIADIASQTHLLSLNAAIEAASAGDAGKGFAVVAS
                     EVKELAKQSAISSQEIKEKILSIQENTENTIEAINQIVKIISRVNDISSTIASAVEEQ
                     SITSKEIASNITQTSIGAREVTKNILGINLAAKSGAERAVQTSDLAKELSELTASLEK
                     IVKSFKI"
     gene            2264..2692
                     /locus_tag="PLF66_15350"
     CDS             2264..2692
                     /locus_tag="PLF66_15350"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HMZ65471.1"
                     /translation="MKNKKLEILTWYVGENLYGLFLKDCKEVIKNVEILPIPHSKNYI
                     SGILNLRGEIITVLDAVVKLGYPKESIRGKKSIIRLKEKSKVSIVADKIYDIIEISSD
                     RLENPPSNLSDRELKCIEKVVNSDQGAIVILNLNGITEIE"
     gene            2696..3763
                     /gene="cheB"
                     /locus_tag="PLF66_15355"
     CDS             2696..3763
                     /gene="cheB"
                     /locus_tag="PLF66_15355"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: protein motif:HMM:NF001965.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis-specific protein-glutamate
                     methyltransferase CheB"
                     /protein_id="HMZ65472.1"
                     /translation="MSEQKKITVIIVDDSDIYRTLVFKILSQSSEIEVISLARSGQLA
                     LPRVRHYKPDIVILDMEMPDLDGLKTLEIIRKDSPDTKVIMFCSKTPESAKQTIRALE
                     LGASDFVTKPEATSGESVDAYIEERLVAKIKAIGRYKPVKAPIPKQETVIPKAKPILN
                     LKGEYDVCAIGISTGGPDALRLLLPNIPKNLDGSIFIVQHMPPFFTKQLAESLNSITE
                     LTVVEGADGMKIQKGYVYIAPGGKHMIVRKASGLELCIGVIDSPPEENCKPAVNVLFR
                     SVAELYGRYAVGVIMTGMGQDGYKGFLEMKKVGAYLMAQDEASSLVFGMPSLPIKEKL
                     VSEVKNIKGLSDSIIRLLGTV"
     gene            3760..4581
                     /locus_tag="PLF66_15360"
     CDS             3760..4581
                     /locus_tag="PLF66_15360"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HMZ65473.1"
                     /translation="MTHLNLTKAIYDLTGIHLQDDKLYLLESRLGDILKKYSLNSFDS
                     LAEKIQSRENPELLREFINSITTQETYFFRDSYIYEAFAFKIMPDLISAYGGEDNIRL
                     SQSKIKIWSAGCATGQEPYSIAILIRETFPNLIGAVEIIASDISLVAIEKAKKGIYLD
                     IEVDRGIDDRLKNKYFKKVDKGYEIDFNIRSMVRFIPLNLHTDQYPREQDVIFCRNVA
                     IYFTKEDRKRIYTNLQRSLKAKGVLTLGSAESLFGFVDNFYIQEFKIARYYQFQK"
     gene            4671..5312
                     /locus_tag="PLF66_15365"
     CDS             4671..5312
                     /locus_tag="PLF66_15365"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HMZ65474.1"
                     /translation="MKIHPMKGVCISIPSSWSYKTAEEILQKNPFWVNNAFRKLSKSE
                     KKSNPFTENTEFKTKYREVKVNSWIGKDVSYTVKPETISIYYPKDRNVLDRDIQEKIR
                     FIIEEMLRKEAKLYLPERLEFLANQYDFKYSGVSIKNMKSRWGSCTSSNRINLNLHLL
                     RIPEELCDYVLLHELCHTVHKNHSSKFWNLLASVCIDAKKKDKEMRNYNAEVY"
     gene            5650..5844
                     /locus_tag="PLF66_15370"
     CDS             5650..5844
                     /locus_tag="PLF66_15370"
                     /inference="COORDINATES: protein motif:HMM:NF025733.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich CWC family protein"
                     /protein_id="HMZ65475.1"
                     /translation="MKTKVLGRKTCPICNSAFDCNVGTDLQCLCSSIDLTHEKKNYLK
                     QKFQDCICADCLRKTSAVNA"
     gene            6366..7169
                     /locus_tag="PLF66_15375"
     CDS             6366..7169
                     /locus_tag="PLF66_15375"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HMZ65476.1"
                     /translation="MAVGYKNVLGAKIYTVEAGVGDPILFLHGNPDTSDLWLYHIDKL
                     KDKYRCIAIDTPGFGRSQIPYRFDFSKEAMIAFMDNLLWELDIKTKVTLIVHDLGGFY
                     GLTYLAKYPEKVSRIGIFDTLYFTNYQWHFWANIWRTNAIGELSMAIMNYPLFLFELK
                     RGSRKLSLAEIQKMYSYDSPNMRKAILDTYREFSPSKFEGWEDKMKSGVKNIPSFVAW
                     GKHDPYIPVEFAYQFGSDNVHILDCGHWTPAEEKEKSVELIQKLFQEKI"
     gene            7175..7738
                     /locus_tag="PLF66_15380"
     CDS             7175..7738
                     /locus_tag="PLF66_15380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65477.1"
                     /translation="MEKTKLQILKESTVYVLLLGFISSILFKLLELFISEEEMLLSNI
                     FLIFIFGFLSFVLTALLMGYFFEKDKPIWIGFLKYSIIGICSTVLTVLVTLILLPFVF
                     DFMVEQVSFFWKDAFQRNIIVGLIMIIPMFIIGIFLAFFGIFAFLFVSYFLGNWIFSF
                     LVQWLFVKYSSKDQKDTKLDSNLTPPS"
     gene            7882..9408
                     /locus_tag="PLF66_15385"
     CDS             7882..9408
                     /locus_tag="PLF66_15385"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HMZ65478.1"
                     /translation="MEIENFNLTKYCFSKNLENNPEKKALIFIHENGKREEKKFAELY
                     NEILSLANHLGKFQLPPFSRVLIRLENNLHYAKLFFASIAQGLVPIPLSPRLTGKEVD
                     FIASDSKASLLIYSKNLEVSKELLQNIQSIPEELLKYEDNENIPQFLFKTQKEDPAFL
                     IYTSGTTGKPKGVLHAHRNIVGRIPMIEGWSGISHNDILLHAGQLNWTYTLGVGLMDT
                     WTRGGTSILYNGSSHPELWLELIEKEKATIFVAVPSLFRRILKHVDIKKYNLESLRYA
                     LTAGEALSIPIYKKWKEQTGKELYEALGMSEISTYISSGPSVPIQLGSPGKAQAGRRV
                     VILPIEGGIEPLKNGEIGLIAVHRSDPGLMLRYWNRPEEESQVYRGDWFIGGDLAHID
                     KEGYIFFHGRNNEIMNSFGYRVSPLEVESVISELPFVQEVAVAEIQESEDLSFITAFV
                     VLKTMEVDIDAVRKEIFLYVKSKLADYKNPRKIYFIESLPRNNNGKLLRKNLSSTLQK
                     "
     gene            9436..9813
                     /locus_tag="PLF66_15390"
     CDS             9436..9813
                     /locus_tag="PLF66_15390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65479.1"
                     /translation="MPKKNKKYKAKSESEKDLYRYEDYNSYTQLPTEQMTLSIDIVSG
                     KYQLTITHIDFGRGGTFAGDTTCYTLTAKLNGRRGQYLFLKVEENQSGFWRKEITLDR
                     KNKTFVPWDARDSRTYPASSYQS"
     gene            complement(9808..10728)
                     /locus_tag="PLF66_15395"
     CDS             complement(9808..10728)
                     /locus_tag="PLF66_15395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65480.1"
                     /translation="MNPNENQFLKCKSCSGHITILDASCPYCGEIIVVDRILSDETKK
                     RISWIAIVLESKIWKKKFILFEKTLFKWGISILLLELILFAVFSILSKSNWKPIVSLL
                     PVSISICAAYYLELYYFYVEEAERSAYKKFIEPIIDEFLIGNNYYKVEFEAILDSIQD
                     NSVSFLKRMISPTINPIQEQDSLSRIVIYHSLLIDKNQYRNSTFQCGYTYIILSFIFG
                     ILSFLILLSINWSVWISVIIPFFWVFLLLEYTDKTSFVFNFFGYKPQKNFIEKEVKPS
                     IQKYCEQTGVSLEEIITKSKDLKKSLHYYL"
     gene            complement(10718..11281)
                     /locus_tag="PLF66_15400"
     CDS             complement(10718..11281)
                     /locus_tag="PLF66_15400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65481.1"
                     /translation="MSQFNLSSDKIGIKMYHSFINCISCGANILNGQTNCPYCQIRLT
                     YTAPDPKQLEWLHDFLKNLSSEIYNKFGTNDSIKKSLSLLWFSIPILFGIFGYITTLD
                     WLIGISLALLLSPVTYILYSIQKESAEYYESEIIQYAKKEILPTLNKELEARNLSQND
                     LIEAIRNVTSRKDYFAHSNLCYLIYES"
     gene            complement(11599..12024)
                     /locus_tag="PLF66_15405"
     CDS             complement(11599..12024)
                     /locus_tag="PLF66_15405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016543991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5360 family protein"
                     /protein_id="HMZ65482.1"
                     /translation="MKTFFNTVEIRLLKFFFLITDLGMIIYWSITYLKLIPEEFLFKD
                     YTNPILMSWNWSFLPLDLFIPLTGLSSLFYIKKNPFLFERLALISLLFTSISGLQAVS
                     FWTIRSDFDPNWWIPNLYLLLYPLYFIYRLLKETKKVSA"
     gene            complement(12021..12815)
                     /locus_tag="PLF66_15410"
     CDS             complement(12021..12815)
                     /locus_tag="PLF66_15410"
                     /inference="COORDINATES: protein motif:HMM:NF016041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HMZ65483.1"
                     /translation="METFLDYFFYWLTGVSISLVLYFGLGSIAFLLFWKWKISKKRIQ
                     EKEKADKAQFKTEIIHSIYSLFLFAIIDMVAYFLQKQGYTQIYEDISTYGWGYFFLSI
                     LFMLILHDAYFYWAHRFMHLKPIYKYIHKVHHESVDTTPYTAFSFHPLEAIVEYGIIP
                     IFIFSFPTHFYSLLIFQILMTGFNVIGHMGYEIYPKNWLQIPILNWKTTSVHHNLHHS
                     KFNGNYGLYFTWWDKWMNTEFPNYESELIRVNQKDNLVTNLIEGKV"
     gene            complement(12904..13461)
                     /locus_tag="PLF66_15415"
     CDS             complement(12904..13461)
                     /locus_tag="PLF66_15415"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HMZ65484.1"
                     /translation="MNYLITTLSATFPLSNSLEEELSKNTKILSLKKKKYLIEKGQTA
                     NHLYFVNEGLLRGFYFQKRKNITTWFAKEQEFVTSIYSFVSRKPLIETIEALEDCQLT
                     SISYEDLQRLYSKHMELNRIGRILMEKYFIELEERAISLQHQTAKERYTHFLNRYSDL
                     FHRVKLGHIASFLGITNETLSRIRS"
     gene            13584..14927
                     /locus_tag="PLF66_15420"
     CDS             13584..14927
                     /locus_tag="PLF66_15420"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HMZ65485.1"
                     /translation="MKNIFLNTKRTKNDLSKEQADQFLLELAESGRKNAFLISIILIL
                     IHIPLLYLDFYRYQWGFWEKQEAFSEIAWMHIILVVVLSFYSIFYLIFRYHSSFWINL
                     YAYIFMSFFIQIWAAVFSAIDQKIFGDMSIYAIAIFGLPYIFHIPIIYEGLKIILAQI
                     VFVYCIFRFNINPDKVDGILINSTVSVILAFVASRINYSYQESRFIQIRKIEEQKIEL
                     EKKNESQDIVLKVVAHDLRNPLSNIIGFISVIRMKYADMNLTGYLDLMEKVSRGAIQI
                     IQDLVEVIGLENNSNPLTFQKIELNQYIEQSIYMNQNMGAKKEIVLNFSPDKGAIFVN
                     ISPEKFSRVIENLLSNAIKFSNTKSEIRIIVKKESDKAKIIIQDFGIGIPFSIQKNLF
                     QKFSNARRRGTEGEMSVGLGMYIVYEIVTLHRGKITFTSEEGKGTTFIIELPIII"
     gene            complement(14920..16188)
                     /locus_tag="PLF66_15425"
     CDS             complement(14920..16188)
                     /locus_tag="PLF66_15425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HMZ65486.1"
                     /translation="MVKELEFATKIIEGDESLLGELEIEINKILSVKKTQYSIYPGYA
                     IFDAWTIVANHAYSSGNLEILKKTFRIFTSAYEKKPYLLPHSLDLHYLLGADGIRHLI
                     HFLEIGFANAPIKGVRILEKGTADKSSYALQLLTSTIERIFAYKEEIDSMQEIAIHIQ
                     NLKWNDDKLWVLFYTIKEIQFSEDLKNPVVANLFLPLLAKGDGEGKKKLEALLKRHGH
                     SLGKSKVQAIHLPDVLPNDPSNAMALLKEWKIGSKELKRIKPASEKKIKELSKKLKVK
                     IPDELEKTLLLHESIGDIEFGPLDRMIALREDIKEILEEHLKEDEIFEFDSDEYRDVR
                     DYNPLTDGIPVGTNTSGDIFFLATNAKSSFGNHPIIRFHHNEALTSSIESNSLGEYIS
                     LIIAKVFAEREGMMDEYYKLSNQKRKITIK"
     gene            16501..16950
                     /locus_tag="PLF66_15430"
     CDS             16501..16950
                     /locus_tag="PLF66_15430"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HMZ65487.1"
                     /translation="MLLNVIERNGNGSSLWNELDRLHDELAGGFFSLGGSTRNYHNPP
                     VNVYINKDEALLSALIPGYDPQSIELSVIENKVQIKGTQPKEVTGEKYELHRQEIDQR
                     DFERTIELPFRVDAEKVDAKFKNGILNVKLPKAEADKPKKISVQLEN"
     gene            16965..17354
                     /locus_tag="PLF66_15435"
     CDS             16965..17354
                     /locus_tag="PLF66_15435"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HMZ65488.1"
                     /translation="MLQQELQKQESKNTNLDKVTRRYITPSVNIYESEEETLILFDMP
                     GVDEKSVDISYEKDVLTVEGKNSYSVPEGFEGSYVGFKTGDYIRKFNVNKPIDLEKSK
                     AVMKNGRLNLHLAKVKPNTTKIEVKTE"
     gene            17468..17833
                     /locus_tag="PLF66_15440"
     CDS             17468..17833
                     /locus_tag="PLF66_15440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65489.1"
                     /translation="MKKLTILPLLLFLLQCTSTANVKLLPTNEVSGIETIWKNKTVGK
                     GSDFSIKVSSFLFEDRNLFFLKDGKPIGKGELKITPSVPLLLLGWIPLGIPYLWLATV
                     SEQQTFDLTVDLKRAAESP"
     gene            complement(17837..18628)
                     /locus_tag="PLF66_15445"
     CDS             complement(17837..18628)
                     /locus_tag="PLF66_15445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002626824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HMZ65490.1"
                     /translation="MQYLESETIELDSILIEKLYLNHEETRNSMTWEMGQEFYEYIES
                     LKKRNPLPRCLIISGRNNIFSSGGNLNFLKSFQEKSFEDNKKDMFTFYNYFLSVRTLP
                     FPVVAAVNGHAIGAALSLAFACDLRVFSMESKYSFNFVKLGIHPGMGSSYLVKELFGL
                     DKANYLLMLAEILTGQEAYKLGLCHDVVPFDDVERRATELAINLGESGPLALRLLKRN
                     LSDYEALQSALRKEAEAQAQNFLSKDFVESLSAIEEKRKAVFKDL"
     gene            18750..20024
                     /locus_tag="PLF66_15450"
     CDS             18750..20024
                     /locus_tag="PLF66_15450"
                     /inference="COORDINATES: protein motif:HMM:NF019245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1577 domain-containing protein"
                     /protein_id="HMZ65491.1"
                     /translation="MAVGRTDSMQELITQMESIYGETIIGTDVNLIKHLFYYLRFDNT
                     EFVFEYDDRIMSVVVHNVLQDFVELNVPDFSEASSRRAKIKFEIINVLYIFEVIIEDI
                     RGSIVKIKLPTELQSAEMRQYRRVACDDLFMDFIILFKSFKGGRYVSGDNIHAERQFT
                     HLFREIKFDVPNLKLMNMIITDYIRRVSDEYELVIYKPGDGHDFIKNTLARDNKTIYV
                     SDCAYVDSYIEDLKSKYYKTFFDIYQDKVKEVGDFQARKFFEVMQKRENRAFMVSYII
                     SPITLFDRVIGHIKVYSTAMDKHMLTTSDLEYIHEMTEIFSYGLTKIAIKGNSFNDIY
                     TNTRIVDISISGLLFEISDESLFQYLKKHSSIKMYIPMGKYKLEINGEIIRYIVADDG
                     FKLGVHFFSSNPGDMKVLETYIYEKKGNVLSE"
     gene            19993..20631
                     /locus_tag="PLF66_15455"
     CDS             19993..20631
                     /locus_tag="PLF66_15455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65492.1"
                     /translation="MKRKAMSFLNKIRFFMILLITTAILTENVYSQEEERVHSLTGIL
                     MDFEEAEIQKNQSKLFTHKAKIFFGKMNESKYAEFLFQGFSTDKSVWTGYKIGDRLEV
                     FFPMAAYLDYSPEKPIPLYKVQKKNPNLERILVYKKEVKDLEIQNHLQTLNCFYTKGM
                     DSSKGKKYYYKTGKKYILRFLTKESFLAFPKLQSKNPLIYEIYIPDWDIQKD"
     gene            complement(20881..21750)
                     /gene="rsmA"
                     /locus_tag="PLF66_15460"
     CDS             complement(20881..21750)
                     /gene="rsmA"
                     /locus_tag="PLF66_15460"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HMZ65493.1"
                     /translation="MSYEYPYYSISKIRNFLESKNANPLKKWGQNFIIDPNILDFIIQ
                     SIDKNALAQSSCLAEIGIGLGALTHKLAQLNQRLVLFEIDPVYIEHMKELEYFQSGKF
                     VLSEGDVLKNLNILKDETVYLTGNLPYYITSDILISSIKSIPHLTGAIFMVQKEFADR
                     ICNEISSLSIFLSIFGKFTYLKKISPTCFYPKPEASSALIQFVPTQNSFTPTQIEKFE
                     LLLKSLFWGKRKTIGKSISEAPFLNSKEFTENYPEIQKEILEAMSNCKIDLKLRPEEL
                     KKEDFHRIVPFLK"
     gene            complement(21740..23869)
                     /locus_tag="PLF66_15465"
     CDS             complement(21740..23869)
                     /locus_tag="PLF66_15465"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HMZ65494.1"
                     /translation="MKFFFQFKPAAQVAYFCVGFLFFPFIQDIRGNLYLFLVSIILFL
                     LFILCGYFRLAATILGFSCSFIYFFILSQDIKSNSLTEEDFYSLSQNKKILITFQKEN
                     KRNFYEAIIKAGTKEFRAIIKRKKKDSLLPSFQCDPNSILLKKESDKEEYFRFLKKFN
                     FQFIQIREKKCEMISYKIDPRKSFKFKTESLLERAGITDYANDIAMGLFFGDASYLEN
                     EFKEKAREGGILHLFAASGLHIGVFMGFLFFFAKRLPFLNYYTERIVPILFAFIYLYL
                     LNFPISLMRAYVFASIYIIGNLFFRKMKSIDLILVSAAILLLLDKESFLTLSFNLSYF
                     AVAGILFLKKNLDELFFGKIKNFFTENFSISLSASLGTYPVLLFYFKSFSFGSIFLNL
                     ILVPLTSLVLPLLYISLFFQFTLEQYLQFIFSWNKNLFNLLTPVTYLFQIGIEILWSY
                     SEFILRSILYLSDHLSSSVGFYRTVFSSYLFHLKFYLFYLILLLLSFILLEKKFFLKE
                     KTSLIFKKYSRRFSILSFLFLICFFYFGYTLYPDSEKNHNNLEVSAGSDYYIVRDKNE
                     IYLGGICKYSEYKIQKLLKEKFCTKTIHSIYIEEESCLSLASLCQSKSIDSNLYSKEK
                     LSDWEKIYPFLSQNQNASKRNFNNLILFYPHIDSLYELQRNTRLEGGKIILLFAYRLN
                     DNTKDWNQNKNLLGINPKWNFITPDEL"
     gene            24632..27211
                     /gene="leuS"
                     /locus_tag="PLF66_15470"
     CDS             24632..27211
                     /gene="leuS"
                     /locus_tag="PLF66_15470"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HMZ65495.1"
                     /translation="MDYPFNEIEKKWQDYWEKNQTFRTDINSSKNKFYCLDMFPYPSG
                     AGLHVGHPEGYTATDIVSRYKRMRGFEVLHPMGWDAFGLPAERYAMQTGIHPSVTTKN
                     NIDNFRRQIKMLGLSYDWDREISTTDPDYFKWTQWIFLKIYNSYFDKRVGKARPIMEL
                     IKRFEEDGTAGTGYANKFTKDDWLRMSLVEKEKVLSDFRLVYQAEIPVNWCEGLGTVL
                     ANEEVEEWTNKGYTVVRKPMRQYMMRITAYAERLLSDLDLVSWPQSTLEMQRNWIGKS
                     EGLDLDFITESGERITVYTTRPDTIFGVTYLVLAPEHPLVDKLTTSENKKAVEEYKLA
                     ASKKSEIDRTELSKEKTGVFIGSYVKSPVGAEGLIPIWISDYVLASYGTGAIMAVPAH
                     DSRDFAFAKKFNLPIKQVIEGPAPVTESFDSKESTCINSFSEELNINGLGYFEASIKV
                     ANWIDARGLGKKKTQYKLRDWLFARQRYWGEPIPLVHYEVGFTRDIPESELPLTLPDL
                     KEFAPSGTGESPLANAKDWLPYKHERFGMGRRETNTMPQWAGSCWYYLRYIDPKNDKA
                     ICDKDLEKKWMSVDLYVGGAEHAVLHLLYSRFWHKILFDLGVVSTPEPFKKLVHQGLI
                     LGEDKKKMSKSLGNVVNPDDVVKEFGADTLRLFEMFMGPFDMVKPWSTKGAEGIFRFL
                     NRVWRLFHGKEGEVFQVDDSEPDLDQLKSLHKTIKKVEEGIENFTFNTAIAQMMIFVN
                     EMTPKQTRPRKVLEPFVLVLAPFAPHIAEELWLKLGNTNTLSYENFPSFNPDYLQEDT
                     ITIVVQVNGKLRGEFTASKTITQEEAIAEAKKLEKVLPFLQDKTIKKEIYVKEKLVNL
                     VVV"
     gene            27225..28097
                     /locus_tag="PLF66_15475"
     CDS             27225..28097
                     /locus_tag="PLF66_15475"
                     /inference="COORDINATES: protein motif:HMM:NF025616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HMZ65496.1"
                     /translation="MRNALIIFSFILILFCKQKEVTNAEVAQAAPKAEKKDFYADGTV
                     ADEEETIGGNKQRKESEEVKNIPGNLDKPLNPITDAAKTRFLEYNIQLSYKVEDINKT
                     RENLLNIVKKDSFISRSQTNLSTGYESMFVQIYIPVGTMYQTLVELDKLGQLTYEDIQ
                     TEDLTDKNEEQKIKLSRESIRGSRRSRVANKGSVENWNWKDREDALERSENAEDQAKL
                     ESWRIKDRVSWAKVNLTLSGKELSNRIQFPNYKNAFVSAINFLLEISYSLVWLVPVLA
                     LGFILFKLYRKIRR"
BASE COUNT         9867 a         4086 c         5004 g         9191 t
ORIGIN      
        1 taagatagga tttgcctttt attttgtggg ttctgtatcg ctactcatag ttggattaac
       61 tagttatttt gtagcaaaga attctttaaa agaagaagct tttaagagag ttacgttagt
      121 aagagaaatg aaagcttctc agattgaaga ctattttcaa atcattcgac atgaaactca
      181 aacctttgca gaaaacaaag cgatagtcga cgcaatgaga gaatttaaaa ttgcatttga
      241 ttcgattgag agagaatctt atataagtat aaagctcgat acagcaaagg aaaacctaag
      301 aagctattat gatcgcgaag tcatcacaag acttaaaaaa tcagcgattg atataaactc
      361 aaattcaata tttccaaaaa cgaattcaga agttatacta caagatcttt atattagctc
      421 taatacaaat gaagtaggaa aaaaaaacct tttaaatgca gctagggaaa gcttatccta
      481 taatactgtc catgctaaat accatcaaaa ttttatggcg attcaacaac gttttggatt
      541 ttatgatatg tttttagtag atgtcgatag tggaaatatc atctatactg tttttaaaga
      601 agtagatttt ggaacttcat tattagatgg tccttacagt aatacaaaca ttggtcgagt
      661 tttcaaagaa actaaaaatt taggacaaaa gggttatgtg aaattagttg attttgaacc
      721 ttatatacct tcttacggag ctgctgctgc gtttatcgca tctccaattt acgatgggaa
      781 taagatgact ggggttttga tttttcaact accaatggat aagatcatgg atgtaatgaa
      841 ctttcaccaa gagtgggaaa aatccggaat gggaaagact ggagaaacgt ttcttgttgg
      901 tcaagactta aagatgaaat ctatttctag attcttagtg gagactcctg aggaatattt
      961 taagtcttta gataagagtg atatgccact tgatgtgatt caaaagatta agaatttaaa
     1021 aacaaatgta ggactccaag aaaacaaaac aaaagcaggt ttagatattg cacgtggaat
     1081 atcaggcaat gaaatctatt tggactaccg tcatgtaaat gttatttcta gttataagcc
     1141 tctacaaatt gaagatgtaa agtgggggat tgtaagtaaa atagatgaag aagaagcatt
     1201 tggcgccgtt tctttgttaa agacaaattt atttttaatt tccttttttg tatttgtatt
     1261 gattggattt tttacactcc tattatcaaa agtaatcatt aagccgattg ctgcgattgt
     1321 ccatcgtata gaagatattg caaatggaga aggagatcta actaagcgtc taacggttac
     1381 atcaaaggat gaactaggag atcttgcaga ttggtttaac aaatttttag taaagattca
     1441 ttcaattgtt ttagaaatcg caagcactag tcagattata ctagttagtt caaatcgact
     1501 cacagataca agtttatctt tatcttcttc tactgaggaa acgtctgttc aatcagagct
     1561 gattgcatcc gctagcactg aaatgagtca aagtgtgcaa gtgattgcat cttcgattga
     1621 agaaatgtct atctcaattg ctgaagttgc aagacgtgct agtgagtatg cggctatctc
     1681 tagagaagca aacaaaacag cgattgaaac aaatgcgatt gtaaatcggt taggagagga
     1741 tgcaaaagat attggaaaag tgatagaaag tattgcggat attgcttccc aaacacatct
     1801 actctcttta aatgctgcga ttgaggcagc gagtgcaggg gatgctggaa aaggatttgc
     1861 tgtagttgct tctgaagtaa aagagttagc aaaacaatct gcaatctctt ctcaagaaat
     1921 aaaagaaaaa atcttatcga ttcaagagaa taccgagaat accatagagg ctattaatca
     1981 aattgttaaa ataatttcgc gggtaaatga tattagctct accattgctt ccgccgttga
     2041 ggagcaatcg atcacatcta aagaaattgc ttctaatatt acacagacat cgatcggggc
     2101 aagagaagtg actaagaata ttttgggaat taatctagcc gcgaaatcag gagctgaaag
     2161 agccgtgcaa acttctgatt tagcgaagga gttgagtgag ttaactgcta gcctagaaaa
     2221 gattgtgaag agctttaaaa tataggacat cgattcccca gaaatgaaaa ataaaaagtt
     2281 agaaatattg acttggtatg ttggagagaa tctatatggt ctttttttaa aagactgtaa
     2341 agaagtaata aagaatgttg aaattttacc aatcccacat tctaaaaatt atatctccgg
     2401 aattttgaat ttaagaggag aaattattac tgttttagat gctgttgtaa agcttggtta
     2461 tccgaaagaa tcaattagag gaaagaaatc gattattcgt ttaaaagaaa agtccaaggt
     2521 ttccatcgtt gcagataaaa tttatgacat tatagaaatt tcatcggatc ggttagaaaa
     2581 tccaccttct aatttgagtg atagggaatt aaaatgtatt gagaaagtag taaattcaga
     2641 tcagggtgcg attgtgatct taaacctaaa tggaattact gaaattgagt agaatatgag
     2701 tgaacaaaaa aaaattacag ttattattgt tgatgattca gatatatata gaacgttagt
     2761 ctttaaaata ttatctcaat cgagtgagat tgaagttatt tcacttgccc gtagtggaca
     2821 actagctcta ccacgtgtgc gtcattataa accggatatc gtcatattag atatggagat
     2881 gcccgattta gatggactta aaactttaga aattattaga aaagattctc cggacacaaa
     2941 agtaattatg ttttgttcaa aaacaccgga gagcgcgaaa caaacaatta gagcacttga
     3001 gcttggtgcc tctgattttg tgacaaagcc agaagcaacc tctggtgaat ctgtagatgc
     3061 gtatattgaa gaaagattgg ttgcaaagat aaaagcaatc ggaaggtata aaccagtaaa
     3121 agctccaatt ccaaaacaag agactgtaat tccaaaggct aagccaattc tcaatttgaa
     3181 aggggagtat gacgtttgtg caataggaat ctctacaggt ggtccggatg cacttagact
     3241 attacttcca aatataccga aaaatttaga tggcagtata tttatagtgc agcatatgcc
     3301 tccttttttt acgaagcaac tcgcagagag tttaaattct attacagagt tgactgttgt
     3361 agagggtgca gatgggatga aaattcaaaa aggctatgtt tatattgccc ccggcggcaa
     3421 acatatgatc gtaagaaaag ctagtggact tgaactttgt attggagtta tcgattcccc
     3481 tcctgaagaa aattgtaaac ctgctgtaaa tgttttattt cgttctgttg cggaactata
     3541 tggtcgttat gcggtaggtg ttatcatgac cggaatgggg caggatggat ataaaggttt
     3601 tttagaaatg aaaaaagtag gagcctattt aatggctcag gatgaagcaa gttcccttgt
     3661 atttggaatg ccctcacttc cgattaaaga aaaattagta tcagaagtta aaaatataaa
     3721 aggattaagt gattctatca ttcgtttatt gggaactgta tgactcattt aaatcttaca
     3781 aaagcaatct atgatctaac aggcattcac ttacaagacg ataaactcta tttacttgaa
     3841 agtaggcttg gcgatatttt aaaaaaatac tctttgaata gttttgattc tctcgcagag
     3901 aaaatacaaa gcagagaaaa tccagaactt ctcagagaat ttataaactc tatcacaaca
     3961 caggagactt atttttttag agactcttat atatatgaag catttgcatt taaaataatg
     4021 ccagatttaa tatctgctta tggaggagag gataatattc gtttatctca atctaaaatt
     4081 aagatatggt ctgcaggatg tgctactgga caggagccat attcgattgc tattttaata
     4141 cgcgaaacct ttcccaattt gattggggca gttgaaatta ttgcgagtga tatttcactt
     4201 gttgcaattg agaaagctaa gaaaggtatt tacttagata tcgaagtaga tcgaggaatt
     4261 gatgatagat tgaagaataa atattttaaa aaagtggata agggttatga aattgatttt
     4321 aatattcgca gcatggtgcg ttttatacca ctcaatctac acacagatca atatccaaga
     4381 gaacaggacg ttatcttctg tagaaacgta gcaatttatt ttaccaagga agatcgcaag
     4441 agaatttata caaacttaca gagatctttg aaggcaaaag gtgtattaac tcttggatct
     4501 gcagagtctt tgtttggatt tgttgataat ttttatattc aagaatttaa aattgcaaga
     4561 tattaccaat tccaaaaata gctgaggatc tattgctttc aaaaacgatt cattataatg
     4621 gcgttggctc cattgagata ataaaatcca aaagaacaaa agggatgagt atgaaaattc
     4681 atcctatgaa aggagtctgt atctccattc catcttcttg gtcttataaa acagcagagg
     4741 aaatattgca aaaaaatcca ttttgggtga ataacgcatt tagaaaactc tctaaatcag
     4801 agaagaagtc taatccattt actgagaata ctgaattcaa aacaaaatat agagaagtga
     4861 aagtaaactc ttggataggt aaggatgttt cttatactgt aaagccagaa acgataagca
     4921 tatattatcc gaaagatcga aatgttttag atcgagacat acaagaaaaa atacgtttta
     4981 ttatagagga aatgcttcgc aaagaagcca agttatacct ccctgagcgt ttagaatttt
     5041 tggcgaatca atatgatttt aaatattccg gagtttctat caaaaatatg aagtctcgtt
     5101 ggggtagttg cacatcttcc aatcgaatca atttaaactt acatttactc agaatcccag
     5161 aagagctttg cgattatgta cttctacatg aactttgcca tactgtgcat aaaaatcaca
     5221 gttcaaaatt ttggaatcta cttgcgtcag tctgtataga cgccaaaaag aaagataaag
     5281 agatgagaaa ttacaatgca gaagtatact aagaattctt cgctctcaat caatagaatc
     5341 attaaagaga aatgaagatt tgatttttga aactcattga ttctatagat tctttcttag
     5401 acttctatcc tgagatatta atgaatagat aaaaggtcta atcaatttta ggtatgacct
     5461 atcaagattg ataggtgttg aatcaaaaaa attgtattct gtattcatct attctatttt
     5521 atttaaaaat tcgttatgga ataaagacta tcggttttag aggttaggaa tatagataac
     5581 gtataaaaaa aaatagaatt attctatttt tattttgaca aaatatataa aactttttag
     5641 gtttcctcta tgaagacaaa ggtattaggt agaaaaactt gccctatttg taattctgct
     5701 ttcgattgca atgtgggaac tgatttacaa tgtttgtgtt cgagtattga tctaacacat
     5761 gaaaagaaaa attatctaaa acaaaagttt caggattgta tatgcgctga ttgtctccga
     5821 aaaacatcag ctgtgaacgc ctaagtcgca tttcctttct ttgacatttg ttaccttttt
     5881 taaaaaatta gatattggta tacagtatct aatttttttt attttactca ccattccttt
     5941 ttcttatctg catagcgatt cctgtgaagc tcttcctaaa ctctaaaaaa ttccagttat
     6001 tctcaatgtc tctatgatct tactgaaagt agaattgagt ttttaaaaaa agcatttcgt
     6061 ctagaaaatc taagtaaaaa aagggaggga gtctatagcg atggggtgga gggagaatgg
     6121 aaataaaaag aaaggcaatg agttttcgat tacctgtgtg cgggatatta cttcacaaac
     6181 tggggagata acaggcatta tcccaaataa aaaataaaat tctctggaga aataatctag
     6241 agaatggtat ctgtgagtcg gaaattccat tttctgaatg taaaatagaa gtagtagaac
     6301 aggactgcat tcgttttcat ggagccaatg ggtatttttc cggaaaatat aggaaggttt
     6361 agtaaatggc agttggttat aaaaatgtat taggagccaa gatttatact gttgaagcag
     6421 gggtgggaga tccaatttta tttttgcatg ggaatccaga tacctctgat ttatggctct
     6481 atcatattga taaattaaaa gacaaataca gatgtattgc gatagataca cctggttttg
     6541 gtagatctca aattccatat cgttttgatt tttctaaaga ggcgatgatt gcttttatgg
     6601 ataatctctt gtgggaattg gatattaaaa cgaaggtgac gcttatcgtt catgatttag
     6661 gaggctttta cggtcttact tatctagcca aatatccaga gaaggtttct agaattggta
     6721 tttttgatac tttgtatttt acaaattacc agtggcattt ttgggcaaat atttggagaa
     6781 cgaatgcaat aggcgaactc agtatggcaa ttatgaatta tcctcttttt ttgtttgagt
     6841 tgaaacgagg atctagaaag ctttctttgg ctgagattca aaaaatgtat tcctatgatt
     6901 ctccaaatat gcgtaaggcg atactcgata cctatagaga gttttctcct tctaagtttg
     6961 aaggctggga agataagatg aaatcgggtg ttaaaaatat tcctagtttt gttgcatggg
     7021 gaaaacacga tccatatatt ccagttgaat ttgcatacca atttggatcg gataacgttc
     7081 atatactaga ctgcggacat tggactccag cagaagaaaa ggaaaagtct gtagaattga
     7141 ttcaaaaatt atttcaggag aagatctgat gcaaatggaa aaaactaaat tacaaattct
     7201 caaagaatct actgtttatg tattgcttct tggctttata agttcaattc tttttaaact
     7261 gttggagctt tttatttcag aagaggaaat gctcctttca aatatctttc ttatttttat
     7321 atttggattt ctctcttttg ttttgactgc tttgttaatg ggctattttt ttgaaaaaga
     7381 taaaccaatt tggattggct ttttaaaata ctcaatcatt gggatttgct ccactgtttt
     7441 gactgtcctt gtgactctta tactgcttcc atttgttttt gattttatgg tagaacaagt
     7501 gtcttttttt tggaaggatg cttttcaaag aaatataatt gtcgggctaa ttatgattat
     7561 ccccatgttc atcattggaa tatttttggc tttctttgga atttttgctt ttctatttgt
     7621 atcttatttt ttagggaatt ggattttttc ttttttagtt caatggcttt ttgttaagta
     7681 ttcttcaaag gatcaaaaag atactaagtt agattctaat ttgactcccc cttcatagaa
     7741 aataaattta gttacaaaca aaatgtttta taaattcgta gactgtaaaa ttagaagtca
     7801 attctagttt tcaaaatatt agaaaatgaa aatcgacaaa taaaaacaat aatagcttgt
     7861 gctagactcg actgatcgtt tatggaaatt gaaaatttta atttaaccaa atattgtttt
     7921 tctaaaaatt tagaaaacaa tccagaaaaa aaggcgttaa tttttattca tgaaaatggt
     7981 aagagggaag aaaagaaatt tgcagagcta tacaatgaaa ttctctctct tgcaaatcat
     8041 ttaggcaaat tccaattgcc tcctttttca agagttttga ttcgtttaga aaataattta
     8101 cactatgcca aattattttt tgcatcgatt gctcaaggac ttgtgccaat accgctttcg
     8161 cctaggctaa cagggaaaga ggttgatttt attgcaagtg attctaaagc ttcccttctc
     8221 atttattcta agaatttaga agtatcaaaa gaattacttc aaaatattca atccattccg
     8281 gaagaattat taaaatatga ggataatgaa aatataccgc aatttctatt taaaacacaa
     8341 aaagaagacc ctgcattttt aatctataca tcaggaacca ccggaaagcc gaaaggagtt
     8401 ttacatgccc atagaaatat tgtaggtaga attccgatga ttgaaggctg gagtggtata
     8461 tcacataacg atatacttct acatgcaggt cagttgaatt ggacttatac gcttggtgtc
     8521 ggacttatgg atacttggac aagaggtgga acttctattt tatacaatgg ttctagtcac
     8581 cctgaattat ggttagagtt gatagaaaaa gaaaaggcta ctatctttgt agcagtgcct
     8641 tctctattta gaagaatctt aaaacatgta gacattaaaa aatacaattt agaatcctta
     8701 cgatatgcgc ttactgcagg agaagcatta tccattccta tttataaaaa atggaaagaa
     8761 caaactggta aagaacttta cgaggctctt gggatgagtg aaatctcaac ctatattagt
     8821 tccggtccaa gtgttcccat tcaattaggt tcacccggaa aagctcaagc tggtagacgc
     8881 gttgttattt tgcctatcga gggagggatt gaaccactca aaaatgggga aatcggactc
     8941 attgctgttc atcgcagtga tcctggactt atgcttagat actggaatcg accagaagaa
     9001 gagtctcaag tatatcgagg agattggttt attggaggag atcttgcgca tatcgacaaa
     9061 gaaggatata tcttctttca tggaagaaac aatgaaatta tgaattcctt cggctatcga
     9121 gtttctccat tagaagtaga atctgtaatt tctgaattac cctttgtcca agaagtggca
     9181 gtagcagaga tacaggaatc ggaggacctt agttttataa ctgcatttgt tgtcttaaaa
     9241 acaatggagg ttgatattga tgcagtgaga aaagaaatat ttttgtatgt aaaaagtaaa
     9301 ctcgcagatt ataagaaccc aagaaaaatc tattttatag aatcacttcc aagaaataac
     9361 aatggaaagc ttcttagaaa aaatttatcc tccactttgc agaaataaaa aaattcttta
     9421 ggttcaaatt ccaatatgcc caaaaaaaat aaaaaatata aagcaaaatc tgaatccgaa
     9481 aaggatctct atcggtatga ggattataat tcctatactc agcttccaac tgaacaaatg
     9541 actttatcga ttgatattgt ttcaggaaaa taccaattaa ccattacaca cattgacttt
     9601 gggagaggtg gaacgtttgc aggggatact acatgttaca ctctaactgc aaaattgaat
     9661 ggaagaagag ggcaatattt atttttaaaa gtagaagaga atcaatccgg attttggagg
     9721 aaggagatta ctttggatcg aaaaaataaa acctttgtcc catgggatgc aagagattca
     9781 agaacctatc cggcgagtag ttatcaatca tagataataa tgcaaagatt tttttaaatc
     9841 ttttgattta gtaattattt cttcgagact aacccctgtt tgttcacagt atttttgaat
     9901 cgatggtttt acttcctttt caataaaatt tttctgaggc ttgtagccaa agaaattaaa
     9961 tacaaacgag gttttatctg tatattctaa aagcaagaaa acccagaaaa aggggataat
    10021 cacacttatc cacacagacc aattgataga caataatata agaaaagaaa gaattccaaa
    10081 gataaaactt aatattatat aagtataacc gcattgaaac gtgctgtttc tatattgatt
    10141 tttatctatg agaagggaat ggtaaataac aatcctagat agtgaatctt gttcctgtat
    10201 tggattgatt gttggcgaaa tcatgcgttt cagaaaactc acactattat cttgtattga
    10261 gtctagaatt gcctcgaatt ctactttata ataattgttt ccaatcaaaa attcatcgat
    10321 gattggttca ataaatttct tataagcaga tctctctgct tcctctacat aaaaataata
    10381 aagttctaaa taataggcag cacagataga tatggaaaca ggtaataaag aaacgatagg
    10441 cttccaattt gatttggata aaatagaaaa gacagcaaac agtattaatt ctaataataa
    10501 gatggagata ccccatttaa ataatgtttt ttcaaagagt atgaattttt tcttccaaat
    10561 tttactctct aaaacaattg caatccagga aattcttttt ttggtttcat cagaaagtat
    10621 tctatcgact acgattattt ctccacagta aggacatgac gcatctaaaa tagtaatatg
    10681 accggagcac gatttacatt ttagaaattg attttcgtta ggattcatag attagataac
    10741 ataaattgga atgagcaaag tagtcttttc tagaagtaac gtttcgaatt gcttcaatta
    10801 aatcattttg acttagattt cttgcttcta attctttatt taaagttggt aatatttctt
    10861 ttttggcata ttgaatgatt tcagactcat agtattctgc gctttctttt tgaatcgaat
    10921 ataaaatata agtaactgga cttagaagta gagcgaggct aatgccgatt aaccaatcca
    10981 aagttgtaat atatccaaag attccaaaaa gaattgggat gctaaaccat aataaagata
    11041 aacttttttt tattgaatca tttgttccaa atttattata aatttccgag gatagatttt
    11101 taagaaagtc atgcagccat tctagttgtt ttgggtcagg cgctgtgtag gtaagtctaa
    11161 tttgacaata gggacaattg gtctgaccat ttaaaatatt tgcgccacaa gaaatacaat
    11221 ttataaagga gtgatacatt ttgataccaa ttttatctga tgatagattg aattgactca
    11281 ttgtaaagtg atttatcaaa aatacaaatt acaatttcaa attttgtaat acattcgtta
    11341 tttatattta cctcctggat tcataggatt tcatcctatt tgaattggta actttaaata
    11401 aatgaaatag tttttttcct ttcactttgg tagaaaaata tgatcgaatt caaatatgga
    11461 taacaaagtc ttctgagaaa gagacatttc ccaaaatata gaaatggatc aaacaacttt
    11521 cgattacagc cattatagtt ttccctctat ctaaacataa gaggaaaaac cgtgatctaa
    11581 aatgaatctt ctttatttct aggcacttac ctttttcgtt tcctttaaga gtcggtagat
    11641 aaaataaaga ggatacagta ataaatataa atttggaatc caccaattag gatcaaagtc
    11701 tgatctgatc gtccaaaaac taacagcttg taaccccgag atactagtga ataaaagaga
    11761 gatgagagca agcctttcaa ataaaaatgg attctttttt atataaaata aactcgaaag
    11821 tcccgtgagt ggaatgaata gatcgagtgg gagaaaagac caattccaac tcataagaat
    11881 tggatttgta taatctttaa aaaggaattc ttctggtatt aattttaaat acgttatact
    11941 ccaataaata atcattccta gatcagtaat gagaaagaaa aattttaaga gacggatttc
    12001 tactgtattg aaaaatgttt tcatactttt ccttctatca aatttgttac tagattatct
    12061 ttttgattta ctcgaattag ttcagattca taatttggaa attccgtatt catccattta
    12121 tcccaccatg taaaataaag accatagttt ccattgaatt tagagtgatg tagattatga
    12181 tgcacgctag tagtcttcca gttcaaaatt gggatttgta accaattttt aggataaatt
    12241 tcatatccca tatgtccaat aacgttaaac ccagtcatta gaatttgaaa aatcagtaag
    12301 ctataaaaat gagttggaaa actaaaaata aaaataggta tgattccata ttctacaatt
    12361 gcttctaatg gatgaaatga gaaagcagtg taaggagttg tatcaaccga ttcatgatgc
    12421 actttatgta tatatttata aattggtttt aaatgcataa aacgatgtgc ccaataaaaa
    12481 taagcatcat gtagtattaa cataaataga atagaaagaa agaaatatcc ccatccatag
    12541 gttgatatat cctcataaat ctgagtatag ccttgtttct gtaaaaaata agctaccata
    12601 tcaattattg caaaaaggaa aagtgaatat atagagtgaa tgatttctgt tttaaattgt
    12661 gctttatctg ctttttcttt ttcttgaatt cttttttttg agattttcca tttccaaaat
    12721 agtagaaatg cgatacttcc taagccaaag tataaaacca aactgatact aacacctgtt
    12781 agccagtaaa aaaaataatc taaaaatgtt tccataataa ttctccattc catatttctt
    12841 aaaagaatag acaatctaaa agatgggttt tgagggttga tttatttgat ctagatcaaa
    12901 aagttaagaa cggattctac tcaatgtttc gtttgtaatt ccaaggaatg aagctatgtg
    12961 tccaagtttt actctatgaa ataaatctga atatctattt agaaaatgag tatatctttc
    13021 tttcgcggtt tgatgttgta aggagatagc tctctcttct aattcaataa aatatttttc
    13081 catcaagatt ctaccgattc gattgagttc catatgcttt gaatataatc tctgtaaatc
    13141 ttcataagaa attgacgtta actgacagtc ttctagtgct tcaatggttt caatcaaagg
    13201 ttttcttgat acaaatgaat aaatagaagt aacgaattct tgttcttttg caaaccaagt
    13261 tgtgatgttt tttctttttt gaaaataaaa tcctctgagt agtccttcat ttacaaagta
    13321 aagatggttt gctgtttgac ctttttctat aagatatttt ttcttcttta gagaaagtat
    13381 ttttgtattt tttgatagtt cttcttcaag agaattactt aaaggaaaag ttgcagataa
    13441 agttgtgatc agataattca tctgctctga tcatcggtca tataaagtgt gatttcctga
    13501 ttttttttct agcagtaaat tgatttcctt tacaggaatc tattgtttaa tacaatcaat
    13561 tacattatca taaatattat taaatgaaga atatattttt aaataccaaa agaaccaaaa
    13621 atgatttatc aaaagaacaa gcggatcaat tccttttaga attagcagaa tctggtcgta
    13681 aaaatgcatt tttaatttca attattttaa ttctcattca tattccactt ttatatctag
    13741 atttttatcg ataccaatgg ggtttttggg aaaagcaaga agccttttct gaaatagcct
    13801 ggatgcatat aattttagta gtagttctat ctttttattc tatattctat ctaattttca
    13861 gatatcatag ctcattttgg atcaatttat atgcttatat ttttatgagt ttttttatac
    13921 aaatatgggc tgcagtcttt tctgcaattg atcaaaaaat atttggggat atgtcaatct
    13981 atgcgattgc gatatttgga cttccctata tttttcatat cccaatcata tatgaaggtt
    14041 taaaaattat attagcacaa attgtttttg tttattgtat ttttcgtttt aacatcaatc
    14101 ccgacaaagt cgatggaatt ttgattaact caacggtatc tgtgattctc gcatttgtag
    14161 catctagaat aaattactct tatcaagaaa gtagatttat ccaaattaga aaaattgaag
    14221 agcagaagat agaattggaa aagaaaaatg aatctcaaga tatcgtgtta aaagtagtag
    14281 cacatgatct cagaaatcct ctttcgaata ttattgggtt tatctctgtt atccgcatga
    14341 aatatgctga tatgaatcta accgggtatt tagacctaat ggaaaaggta agtcgtggag
    14401 ctatacaaat tattcaggat ttggtagaag tcattgggtt agaaaataat tcaaatcctc
    14461 taacgtttca aaagatagaa ttaaatcaat atatagaaca aagcatttac atgaatcaaa
    14521 atatgggagc gaaaaaggaa attgttttaa attttagtcc agataaagga gcgatttttg
    14581 taaatatctc tcccgaaaaa ttctcacgtg tgattgagaa tttactatcc aatgcaatta
    14641 aattttcaaa tacaaagagt gaaatcagaa tcatagtaaa aaaagaatct gataaagcga
    14701 aaataattat tcaagatttt ggcattggaa ttcctttttc catacagaaa aatctttttc
    14761 aaaagttttc aaatgctaga agaagaggaa cagaaggaga aatgtcagtt gggcttggga
    14821 tgtatattgt atatgaaatt gtcactctac atagaggcaa aataacattt acctctgaag
    14881 aaggaaaagg cactactttt attatagaat taccaattat tatttaatcg taatctttct
    14941 tttctgattt gagagcttat aatattcatc catcattcct tctctctctg caaatacttt
    15001 cgcaattatg agagaaatgt attctccaag agaattggat tcaatgctgg aagtgagtgc
    15061 ttcattgtga tgaaatcgta taattggatg gttcccaaag ctagattttg cattggttgc
    15121 aagaaagaaa atatctccac ttgtatttgt tccaacggga ataccatcgg ttaacgggtt
    15181 atagtctctt acatctctat actcatctga atcaaactca aaaatctcgt cttcttttaa
    15241 atgttcttcc aaaatttctt ttatatcttc tctgagtgca atcatcctat caaggggacc
    15301 gaactctata tcgcctattg attcgtgaag caaaagagtc ttttctagtt catcaggaat
    15361 ttttactttg agttttttag aaagttcttt aattttcttt tcagaggctg gctttattcg
    15421 cttcaattct ttggatccaa ttttccattc ttttaataga gccattgcat tactcggatc
    15481 attcggcaat acatctggta gatgaatggc ttggacttta gatttgccaa gagaatgtcc
    15541 gtgtcttttg agtaaggctt ctaatttctt tttgccttcg ccatctcctt tcgcaaggag
    15601 tggtaaaaat agattagcta ctactggatt ttttaaatcc tctgagaatt gaatttcttt
    15661 gatcgtataa aataataccc aaagtttatc gtcattccat tttaagtttt gtatgtgtat
    15721 tgctatttct tgcattgaat caatttcttc tttataagca aatattcttt ctattgtaga
    15781 cgtaagaagc tgtaaggcat aagatgattt gtctgctgtt cctttttcta aaatcctaac
    15841 acctttaatc ggggcatttg caaatccaat ctctagaaag tgaatgagat gcctaattcc
    15901 atctgcaccc aataaataat gtaaatcaag agagtggggg agtagatagg gttttttctc
    15961 ataggcagac gtgaagatcc gaaatgtctt ttttagaatt tctaaattac cggaactata
    16021 agcatgattt gcgactatcg tccatgcgtc aaaaatcgca taacctggat aaatactgta
    16081 ttgagttttt ttaacagata ggattttatt tatttctatt tctaattcgc caagaagaga
    16141 ttcgtctccc tctattatct ttgtagcaaa ttcgagttcc tttaccataa gtaggttttc
    16201 ttcatttatt atggaagggc aagaatttat aaaggaagga ctgaataaat tagagattta
    16261 gagttcttta atcttgaagg ttttttgaaa aaaggaacga agttacccac tgtgtataag
    16321 ttggatcttt ttttagagct tttaactgcc ccaaaaaagt agattttttg aaaaaattta
    16381 tccaaaaacg caattttttt tatttacaaa atgaactttt ctatgtatta gcactctata
    16441 tatatgagtg ctaaaaataa ttagcactta atagactaga gtgctaaagg agaaacttat
    16501 atgctattaa atgtaattga gagaaatgga aatggttctt ccctatggaa tgagttggat
    16561 agactgcatg atgagcttgc aggcggtttt ttctcccttg gaggctcaac cagaaactat
    16621 cataatccac cagtaaatgt gtatattaac aaagatgagg ctttgttgtc tgctttgatc
    16681 cctggttatg acccacaatc aattgaatta tcagttattg aaaataaggt tcagatcaaa
    16741 ggaactcaac caaaagaagt tactggagaa aaatacgaat tgcatagaca ggaaatcgat
    16801 caaagagact tcgaaagaac catcgaactt ccgtttagag tagatgctga aaaagtagat
    16861 gcaaagttta aaaatggaat tttgaacgta aagcttccta aagcagaagc agataaacct
    16921 aagaaaattt ctgttcagtt ggaaaactaa gaaaggagtg aaatatgtta caacaagaac
    16981 tacaaaaaca agaaagtaaa aatacaaatt tggataaagt aactcgcaga tacataacac
    17041 catctgttaa tatctatgaa tcagaagaag aaacattgat tctttttgat atgccaggag
    17101 tggatgaaaa atctgtggat atttcctatg aaaaagatgt tctcactgtt gaaggtaaaa
    17161 actcctattc agttccagag ggctttgaag gaagctatgt agggtttaag acaggggatt
    17221 atattcgaaa attcaatgtc aataagccta ttgatttaga gaaatctaaa gcggtgatga
    17281 aaaatggaag attaaacctt caccttgcaa aagtaaaacc aaacacgact aaaattgagg
    17341 ttaagaccga gtagcctcta acctatttcc ctattattca ttttgggtcg agccaacctc
    17401 tcttggttcg accctttttt tgctataaac tcttgacaat atcatttctg aaagaaatgg
    17461 ttgccctatg aaaaaattaa ctatactacc attattactc tttttactcc aatgtacctc
    17521 tacagctaac gttaagttgc taccaacaaa tgaggtttct ggaatagaaa cgatctggaa
    17581 aaataagact gttggaaaag gttctgattt ttctataaag gtctccagct ttctttttga
    17641 agacagaaat ctgttttttt taaaagatgg aaaaccaatt ggaaaagggg aattaaaaat
    17701 tactccctca gttccacttt tacttttagg atggattcca cttggaattc cttatctatg
    17761 gcttgccact gtttctgaac aacaaacctt cgatttaact gtagatctaa aaagagcagc
    17821 tgaaagtcct taattattat aagtctttaa aaactgcctt tctcttctct tcaatagccg
    17881 agagactttc tacgaaatct tttgataaaa agttttgtgc ttgtgcttcg gcttcttttc
    17941 gaagggcact ttgtagtgct tcatagtcac ttagatttct ctttagaagt ctgagtgcaa
    18001 gaggaccact ttcccctaaa ttgatagcta gttctgttgc ccttctttct acatcatcaa
    18061 aaggcacaac atcgtggcag agacctagtt tataggcttc ttgtcctgtt aaaatttctg
    18121 caagcataag caaataattt gctttatcca aaccaaatag ctcttttact aaataagaag
    18181 aacccatccc tgggtgaatt cctagtttaa caaaattgaa agagtattta ctttccattg
    18241 agaaaacacg tagatcacag gcaaaagcaa gagaaagtgc ggctcctata gcatgaccgt
    18301 ttacagcggc aacgaccgga aaaggaagag tccgaactga taggaagtag ttatagaaag
    18361 taaacatgtc ctttttattg tcttcaaagg acttttcctg aaaggatttt aaaaagttca
    18421 gatttccacc agatgaaaaa atattatttc tacctgatat gatcaggcaa cgaggcaaag
    18481 gatttctttt tttgagggat tcaatgtatt cgtagaattc ttgtcccatt tcccaggtca
    18541 ttgagtttct tgtttcttcg tgatttagat ataatttttc gattagaata gagtctagct
    18601 cgattgtttc tgattctaaa tattgcataa attcttccta tattctagat tttatccctc
    18661 tcgtgtggat tctgtaacaa tttgaaaaaa cataaaaatt tgcaaattgt aatttgctaa
    18721 tttgcatatt ttttgacgat atttaattta tggcagtcgg aagaacagac tcaatgcagg
    18781 aattgattac ccagatggaa tccatctacg gggaaacaat tattggcact gatgtgaact
    18841 tgattaaaca tcttttttat tatctacgtt ttgataatac agaatttgta tttgaatatg
    18901 atgatagaat catgtccgtt gttgtgcaca atgttctgca ggattttgtg gaattaaatg
    18961 ttcctgactt ttctgaggca agttcaagaa gagcaaagat caaatttgag attatcaatg
    19021 ttttatatat ttttgaagta attattgaag atatacgtgg ctccattgta aaaataaagt
    19081 taccaactga attgcaatct gctgaaatgc gtcagtatag acgtgttgcc tgcgatgatt
    19141 tatttatgga ttttataatc ctgtttaagt ctttcaaagg gggacgttat gtgagtggcg
    19201 ataacatcca tgcagaaaga caatttactc atctctttag agaaatcaaa tttgatgttc
    19261 ccaatttgaa actgatgaat atgattatta cagattacat tcgaagagtt tctgatgagt
    19321 atgagttagt aatttataaa cctggggatg gtcatgattt tataaagaat acacttgcta
    19381 gagacaataa aactatttat gtttctgatt gtgcttatgt agattcttat attgaagact
    19441 taaaatccaa atattataaa actttctttg atatttacca agataaagtg aaagaagtcg
    19501 gagactttca agctagaaaa ttttttgaag taatgcaaaa aagagaaaat agagccttta
    19561 tggtttctta tatcatttct cctatcacac tttttgacag agtcattggg catattaagg
    19621 tctacagcac agcgatggat aagcatatgc tcactactag tgatttagaa tatatccatg
    19681 aaatgacaga gatttttagt tatggtctta ctaaaattgc aattaaggga aatagtttca
    19741 atgatattta cacgaataca cgaattgtag atattagcat tagtggttta ttgtttgaga
    19801 tctctgatga aagtctattt caatatctaa aaaagcattc tagtattaaa atgtatatcc
    19861 caatgggaaa atataagttg gaaatcaatg gggaaattat acgttatata gtcgcggatg
    19921 acggatttaa actaggcgtg catttcttct cttctaatcc tggagatatg aaagttttag
    19981 agacctatat ctatgaaaag aaaggcaatg tcctttctga ataagattcg attttttatg
    20041 attctactta taacgactgc gattcttaca gagaacgtat acagtcagga agaggaacgg
    20101 gttcattctt taactgggat tctaatggat tttgaagaag cagaaattca aaagaatcaa
    20161 tcgaagttat ttacccataa agcaaaaatc ttttttggga aaatgaatga atcgaaatat
    20221 gctgagtttt tatttcaggg cttttcaaca gataagtcgg tttggacggg gtataaaatt
    20281 ggagatagat tggaagtttt ctttcctatg gcggcatatt tagattattc tccagaaaaa
    20341 ccgattccac tttataaagt gcaaaagaaa aatccaaatc tagaacgtat acttgtttat
    20401 aagaaagaag taaaagattt ggaaatacaa aatcatttac aaactctaaa ttgtttttat
    20461 acaaaaggaa tggattcttc gaaaggaaaa aaatattatt ataaaacggg aaaaaaatat
    20521 attcttcgct ttctgactaa ggaatcattt cttgcctttc ccaaactgca gtcaaaaaat
    20581 cctttgattt atgaaatcta tattcctgat tgggatattc aaaaagacta attttctaat
    20641 tcgaaagttt tatcaataaa tggacacatt tattttaacg tgagttcggt gtaagacatg
    20701 tttcttttga tcacccccga attccacccg atgctattcg catccaatac cgacggggcg
    20761 attatcgggg atatctagat ctatagattc ccgatatcgc ttggcggttg cggcgatagc
    20821 gggtggaatt tcgggaatga cggtctaaat aatttgtcaa atccttatat cgaattcatg
    20881 ttattttaga aaaggaacga ttctatgaaa atcttctttt tttaattctt cgggtcttag
    20941 ttttaaatca atcttacaat tactcattgc ttctaaaatt tctttttgaa tctccggata
    21001 attttcagta aattcttttg agtttaaaaa gggagcctct gaaatagatt tgcctatcgt
    21061 ttttcttttt ccccagaaaa gagatttgag tagaagttca aatttttcga tttgcgtggg
    21121 agtaaaactg ttttgggttg gaacaaattg aatgagtgca gaacttgctt ctggctttgg
    21181 ataaaaacaa gtaggagaaa ttttttttaa gtaagtgaat tttccaaata tggataggaa
    21241 gatagaaaga gaggaaatct cattacaaat tctgtctgcg aattcttttt gcaccataaa
    21301 gattgcgcct gttagatggg gaatcgactt gatcgaggaa attaaaatat cacttgtgat
    21361 atagtaagga agatttccgg tcaaataaac agtctcatct tttaaaatat ttaaattttt
    21421 tagaacatct ccttcggata atacgaattt gccgctttga aagtattcta attctttcat
    21481 atgttcgatg tagactggat caatttcaaa taggactaat ctttgattga gttgtgcgag
    21541 tttatgagtg agagcgccga gtccaatccc tatttctgcg agacatgaac tttgagccaa
    21601 agcattttta tctatcgatt gaatgataaa atctagaatg tttggatcta taataaaatt
    21661 ttgtccccat tttttaagcg ggtttgcatt tttagattct aagaaatttc taattttaga
    21721 aatactataa tatggatact cataactcat ctggtgtaat aaaattccac ttgggattta
    21781 ttccaagtaa gtttttattt tgattccaat cttttgtatt gtcgtttagt cgatatgcaa
    21841 ataataaaat aatttttcct ccttcgagtc ttgtattcct ttgaagctca tataaagaat
    21901 ctatatgagg gtagaagagg atcaggttat tgaaatttct tttggaggca ttttgattct
    21961 gagagaggaa gggataaatc ttttcccaat cagatagttt ttcttttgaa tatagattgg
    22021 aatcaattga tttggactga catagacttg caagggaaag acaggattct tcttctatgt
    22081 agatggagtg gattgttttt gtgcaaaact tttcctttag taatttctga attttgtatt
    22141 cactgtattt acagattccc ccaagataaa tttcattttt atctctcaca atataataat
    22201 cagagccagc agagacttct agattattat gatttttttc cgaatctgga taaagagtat
    22261 aaccaaaata gaaaaaacaa attaaaaaaa gaaaggatag aattgaaaat ctacgactgt
    22321 attttttgaa aatcaaagaa gttttctctt ttaaaaaaaa ctttttttcc agtagaataa
    22381 aacttaataa aagtaagatt aaatagaata gataaaactt taaatgaaat agataggaag
    22441 aaaatacggt gcgatagaat ccaacggaag aggagaggtg atcggaaaga tagagaatac
    22501 ttctcagaat gaattctgaa taagaccaaa gtatttctat tcctatttga aatagataag
    22561 tgactggagt taatagattg aataagtttt tattccatga aaatataaat tgtaaatatt
    22621 gttccaaggt aaattggaaa aaaagagaaa tatagagtag tggcaggact agagacgtta
    22681 agggaactaa aattaaattt aaaaaaatag aaccaaaact aaacgattta aaatagaaaa
    22741 gtaaaacagg ataagttcca agacttgcag aaagagagat agaaaaattt tccgtgaaga
    22801 agtttttaat tttcccaaag aataattcat ccaaattttt tttcaaaaat aaaattccag
    22861 caaccgcgaa ataagataaa ttaaaggaga gtgttagaaa actctcttta tctaagagga
    22921 gtagaatggc agcagaaact aatattaaat caatggattt catttttcga aaaaacagat
    22981 tgccgattat gtaaatagac gcgaatacat aagcgcgcat aagagaaatg ggaaaattca
    23041 aaagatataa atagataaat gcaaataaaa taggaacgat tctctctgta taataattta
    23101 aaaaaggaag tctctttgca aaaaaaaata gaaaacccat aaacacaccg atatgaagac
    23161 ctgaggccgc aaataaatga agaattcctc cttctctagc tttttcttta aattcatttt
    23221 ctaaataact cgcatcacca aaaaacaaac ccattgctat atcatttgca tagtcggtta
    23281 taccggctcg ttctaataaa ctttcggttt taaatttaaa actttttcta ggatcaattt
    23341 tatacgagat catttcacat tttttttctc tgatctgaat gaattgaaaa ttgaattttt
    23401 ttaaaaatcg aaagtattct tctttatccg attctttttt taatagaata gaattgggat
    23461 cacattgaaa actaggaagt aaggaatctt tctttttcct ttttataatt gctctaaatt
    23521 cttttgtgcc tgcttttatg attgcttcat aaaagtttct tttattttcc ttttggaatg
    23581 tgattaatat ttttttgttt tgagataggg aataaaagtc ttcttctgtt agggaattcg
    23641 actttatgtc ttgtgaaaga atgaaaaaat aaataaaact acaagaaaat ccaaggattg
    23701 ttgcggctaa tcgaaagtat ccgcaaagaa taaaaagcaa aaataaaata atggatacta
    23761 aaaaaaggta taagtttccc cttatgtcct gtataaaagg aaaaaataaa aatccaacac
    23821 agaaataagc tacttgtgcg gctggtttaa actgaaaaaa gaatttcata tacataaaaa
    23881 gagaatgaaa acttcaagaa tgtgcacaaa aaattcaaaa gattacgtat ttgcacaaaa
    23941 aaaattttta aaatttaata tcttggaagt aaaaacttag ttatgaaaga tttatatatt
    24001 agtatattag aaaatactaa tataatttga gtaaataaaa tcaatattga ttgagaaaat
    24061 taaaattttg aaacaaaaaa tcaaaaaagt atattatgaa gatgaaattc aaaaaataat
    24121 tttcattcct gttctattca gaaaaatttt aaaaaaaata tttttttgat cttttttttt
    24181 ctcactaaat taatattatt tttgaccttt taggtagaaa aaaatacaac gtaatcccta
    24241 ccacgagtga atttatccac ttaggtagat gttattgact gaagttattg acacactgta
    24301 aaaaaatatg acagagaaac ctatgaagat attacacgaa gaagaatcaa tttttaacaa
    24361 agataaaaaa atggaaattg aaatattata caaatgttcc ggctgttcaa agatggaaaa
    24421 aaaactatac cagcaaagac tttgtaaggc atgtttatca aaagctttta agaggttaat
    24481 aaaagtaatc gactcagtaa gaaaataatt tccttttatc ccacagctaa agaaatggta
    24541 aatacaatcg gttcggttgt ataattttta acttacagat ttttttattt agatatttat
    24601 ttatcaaata tagaaaaaag aacgggctaa catggattat ccttttaatg agatcgaaaa
    24661 aaaatggcaa gattattggg aaaaaaatca aacattccga acggatataa atagtagtaa
    24721 aaataaattt tactgtttgg atatgtttcc ttatccaagt ggagcaggtc tccacgtagg
    24781 tcatccagag ggatacactg caacagacat agtttccaga tacaaaagga tgaggggatt
    24841 tgaagttctc catcctatgg gatgggatgc ttttggtctt cctgcagaac gctatgcaat
    24901 gcagactggc attcatccga gtgtcacaac aaaaaacaac atagataatt ttagacgcca
    24961 aattaagatg ctcggtcttt cctatgattg ggatcgagag atttcgacta ccgatcccga
    25021 ttattttaaa tggactcaat ggatattctt aaaaatatat aattcttatt ttgataaacg
    25081 agtcggaaaa gccagaccga ttatggagct tatcaaacga tttgaagaag atggcacagc
    25141 gggcacaggc tatgcaaata aatttacaaa agatgattgg cttcgtatgt ctcttgttga
    25201 aaaggaaaaa gtcctttcag attttcgttt agtttaccaa gctgaaattc ctgtcaactg
    25261 gtgtgaggga cttggaactg ttcttgcgaa tgaagaggtt gaagaatgga cgaacaaagg
    25321 ttataccgtt gttcgaaagc ctatgcgtca gtatatgatg cgtattactg cctatgcaga
    25381 aagactttta agcgatttag atttagtctc ttggcctcag tccaccttag agatgcaaag
    25441 aaactggatc ggtaaaagtg aaggtttaga tttagacttt attactgagt ctggagaacg
    25501 tataacggtc tatacaacca gacctgatac aatttttgga gtaacctatt tagtattagc
    25561 tccagagcat cctcttgtag ataaactgac aacttctgaa aataaaaaag cagtcgaaga
    25621 atataaatta gccgcatcta aaaaaagtga aattgataga acagaactca gtaaagaaaa
    25681 aacaggagtc ttcatcggat cttacgtaaa aagtcctgtg ggcgcggaag gtcttattcc
    25741 gatttggatt tcagattacg tcttagctag ttatggaaca ggagctatta tggcggttcc
    25801 agcacacgat agtcgcgact ttgcctttgc aaaaaaattt aatttaccaa ttaaacaagt
    25861 cattgaagga cctgctccag ttacagaaag ttttgattca aaagaatcaa cttgtatcaa
    25921 ctcatttagt gaagagttaa atatcaatgg actcggttat tttgaagcct ccattaaagt
    25981 tgcaaattgg attgatgcaa gaggtcttgg aaaaaagaaa acacaataca aattaagaga
    26041 ttggcttttt gctagacaga gatattgggg ggaaccaatt ccactcgtgc attatgaggt
    26101 tggttttaca cgcgatattc ctgaatcaga actccctctt actttacctg acttaaaaga
    26161 atttgctccc tctggaacag gagaatctcc tttagccaat gcaaaagatt ggcttcccta
    26221 taaacacgaa aggtttggaa tgggtagaag agagacaaat actatgcctc agtgggcggg
    26281 ctcttgttgg tattatttaa gatatataga tccaaaaaat gataaagcga tatgcgacaa
    26341 agatttagag aagaaatgga tgtccgtcga tttgtatgta ggaggagctg aacacgcagt
    26401 tcttcatttg ctttattcta gattctggca taagatttta ttcgatttgg gcgttgtatc
    26461 gactcctgaa ccttttaaaa agttagttca ccaaggactt atcttgggag aagacaaaaa
    26521 gaaaatgtct aagtccttag gtaatgttgt aaacccagat gatgtagtaa aagaatttgg
    26581 agcagataca cttcgtcttt tcgaaatgtt tatgggacct tttgatatgg taaaaccttg
    26641 gagcacaaaa ggtgcagagg gaattttcag attcttaaac agagtatgga gactctttca
    26701 tgggaaagaa ggtgaagtat tccaagtgga tgattccgaa ccagatttag atcaattaaa
    26761 gagtcttcat aaaacgatta agaaagtaga agaggggatt gagaatttta catttaacac
    26821 tgcaattgcg cagatgatga tttttgtaaa tgaaatgact ccaaagcaaa ctcgtcctag
    26881 aaaagtttta gaaccatttg ttttggttct cgctcctttt gctcctcata ttgcggaaga
    26941 actttggtta aaattaggaa atacaaatac actttcctat gaaaattttc catcctttaa
    27001 tccagattat ctacaagaag atacaatcac aattgtagtg caagtaaatg gaaagttaag
    27061 aggagagttt accgcttcca agacaataac acaagaagaa gcgattgcag aggcaaaaaa
    27121 attagaaaag gttcttcctt tcttacaaga taaaacaata aaaaaagaga tttacgtgaa
    27181 agaaaaatta gtgaaccttg ttgtagtcta gtaaggggac aatcatgaga aatgctctaa
    27241 ttatttttag ttttatttta attctatttt gcaaacagaa agaagttaca aatgcagaag
    27301 tagcgcaagc tgctccgaaa gcagaaaaaa aagatttcta tgcagatgga acagttgcgg
    27361 atgaagaaga aacaatcggt gggaacaaac aaagaaaaga aagtgaggag gtaaaaaata
    27421 ttccgggtaa tctggataaa ccgctaaatc caattacaga cgctgccaag actcgtttcc
    27481 ttgaatacaa tatccaactc agctataaag tagaggatat aaataagact agagagaacc
    27541 tactaaatat tgtaaaaaag gattctttta tttctagatc acagactaat ttaagcactg
    27601 gatatgaatc tatgtttgtg cagatttata tccctgtcgg gactatgtat caaactttgg
    27661 ttgaattaga taaattaggg caactaacgt atgaagacat ccaaacagaa gacttaactg
    27721 ataagaatga agaacagaaa attaaactta gcagagaatc gattcgagga agtagaagat
    27781 ctagagttgc aaacaaaggt tctgttgaaa attggaattg gaaagataga gaagatgcac
    27841 tcgagagaag tgaaaatgca gaagaccaag ctaaactaga atcctggaga atcaaagata
    27901 gagtttcttg ggcaaaggtc aatctgactc tatcagggaa agaattatct aaccgaattc
    27961 agtttccaaa ttacaaaaat gctttcgtaa gcgcgattaa ctttttactc gaaatatctt
    28021 attcacttgt atggttagtt ccggttctag ctttaggatt tatattattt aaactctatc
    28081 gaaagattag aagataagaa aattaaaaat cacatctttt cacaaatagc aaaagatgtg
    28141 aaaaaaat
//
LOCUS       DAOQOS010000071        28043 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_767_length_28043_cov_5.603794, whole genome shotgun sequence.
ACCESSION   DAOQOS010000071 DAOQOS010000000
VERSION     DAOQOS010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 28043)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28043)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28043
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_767_length_28043_cov_5.603794"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..288)
                     /locus_tag="PLF66_15480"
     CDS             complement(<1..288)
                     /locus_tag="PLF66_15480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65497.1"
                     /translation="MGSFLFAAIYCCLLGCKAGSAFPKIFGIFPIKPTSAPAQFVISP
                     STGLQTSESGTSLQIRISLNKAPTDTVSIQTISFSDSKEGTIDKTSLIFTTS"
     gene            685..1461
                     /locus_tag="PLF66_15485"
     CDS             685..1461
                     /locus_tag="PLF66_15485"
                     /inference="COORDINATES: protein motif:HMM:NF012970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HMZ65498.1"
                     /translation="MIKIWIPFLILFSTNLLKAKETISAKSYLIEDRDTKVILSKKNT
                     SIVLPMASITKLATALLTLKNKDLNDFVKITKTTVLRNETDKSAFLTEGEFYTRKDLL
                     LTMLVSSTNVAAISLAANVSGSPEKFVEDLNLWAKENQLEKTSFGDTFGLSQNSKSNI
                     DDIAKILDLIESNKDLFTILTTTSYTITEKKGKKQNLTTTNEMPTYKEYKIFGKTGTT
                     NAAGKCFAGFITKDSKKWKIILLGSKNVFSDMKILIDQIQ"
     gene            1481..2965
                     /locus_tag="PLF66_15490"
     CDS             1481..2965
                     /locus_tag="PLF66_15490"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HMZ65499.1"
                     /translation="MQFSNLDFLVLISYLAIVLLAGLHSSKNDTSKSFFLADKELPWY
                     FIMLSIVATETSSLTFLNIPGLSFKGDFSFLQVAFGYIIGRAFVANFILPLYYKGGYS
                     SVYEWVGEKFGKETQKSVSSVFLITRVLGDGIRLYATSIPIAIIFHGYFQNLVSEETT
                     GILTLFIITMITTLYTVFGGFKSVVVTDSLQFFIYIGGGVFSFVYLMQDLGNHISFSE
                     ILSTSLSNGKLNFYQGSTGITTSNFFQKPYFFINGLIGGILLSIGTHGVDQMFAQRLI
                     ACKSEKESKLALIGSGILVFLQFVLFLGIGLLLFYKYQGSEIHPDKVFSKYIIENIPT
                     PILGIIVAAILASAMSTLSSSINSMSLSVLFDWMKKNESEHQRVLLSKWISVLWGFVL
                     FGSSLLPYYFSGKTSEGLVEIGLKIASFTFGPLTALFLLVRLKDRFALNLYPSLLIFS
                     LFLSLVTTILFSISLKPAMAYLIPFGIFIFYLTIFTGQFLKPKV"
     gene            3246..6308
                     /locus_tag="PLF66_15495"
     CDS             3246..6308
                     /locus_tag="PLF66_15495"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HMZ65500.1"
                     /translation="MIHIKIMLVFLILSLSHCLGIKSSQEKPKVTSKEGILDLRNWDF
                     YKYGMVELNGDWEYYPEKFDLGENTNEKKPKSFYEFGSKINPNQAYPEYASFRLKILL
                     PENKPELLFFRIAGLNSSYNLYANFKLIAFRGIAGKSKLTTTPSIGSDISQGILDSKE
                     INLILQVSDFYSNKHGFSGKLELGTMSELGSSANLELLTSFGLVLFLSFVFLISITQI
                     SERIFLLLSISSIGLIFHISLSYPYPIRVIASKTREDFIYYFRIFNQGLVTICFAYFY
                     YQICEELKSIWAKISITGAFSVFILFLFLSSNYIPPKLEIISSFYLAFTQIVFTYHFF
                     LDGPSSFARIKAYTKKYKAELDRTLGELTDSKKDFDKKIIERSFNMVTYMEKLEIQTG
                     EMEKLNELIIHLLEGANINSVLDEIFSHIMTYYRSNIAFLYFLDPVTNEFFPYRGITK
                     DIPEEIRSFMENSRMPVSREAGVAYIAYKRKKTIYLENAKTKYTRKREKGKGIPELVS
                     SLYIPVLIRNEFQGIFFLATFNHPLNLNKEKIKFISMFTNQLTAAIQKEKLLKEMEEE
                     KRKAEMAREFAEIAKKEAEEAKEEIEAINSLTKSINENLELKYIMDKIMNFVKDHYGI
                     HYYSLHILNDNKKKMKLLEAKFPDHVTSEEKRRVSQIHIPVQDSEGAFPFIFKTKKMI
                     YYKKLNIDKSNEEERESLRNLHISSMVGIPLKVKNEIIGILNFFSPEELSLTKTHLKT
                     IANLGEQVAGVIHNSSLYKDIKVEKDKSEGLLLSILPPKIATELKNTSKVVPIIYESV
                     TILFTDFVGFTKIAETLLPKQLLIELDGYFTFFDFVCEKYNIEKLKTIGDSYMCAGGL
                     PTANKTHAIDACLTAIEFRDFAIRMQELNATSKDEMMPWELRIGLHTGPVIGGIVGTT
                     KFAYDVWGDSVNIASRIESSSVPGRINISETTYELVKDFFICEHRGKVSAKNKGEIRM
                     YFLNRLKPEFSANEEGTAPNQKFYEYYEKIKLGQPIPIQSSSKLRLVV"
     gene            complement(6593..8737)
                     /locus_tag="PLF66_15500"
     CDS             complement(6593..8737)
                     /locus_tag="PLF66_15500"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HMZ65501.1"
                     /translation="MNTNVKITRTNSGTPKIKANTIEECLYGLGYVHAVDRGMQMVFM
                     KILGQGRLSELLDSSDHSLEIDKFFRRMNWYGRTKKPLAEVDKEKKQWLKSYCDGVND
                     GFAESFPFEFKLLGVPKEAWTIEDSILISRMIGYLTLAQSQAEIERLFIEMVQAGLSE
                     NHLKELFPNILDGAKFDSIKKIKLEDRVVPSSLWEMGAPKMMASNNWVIGGKKTKSGK
                     PILANDPHLEINRLPSVWGEVILSTSDWYAIGGSMPGVPGILTGRTNHLSWGVTYAFI
                     DAYDSWMEDCKEGKYLKEGKYTPFIIRKESIKRKKKKTEEVIFYENEHGTLSGDPFKE
                     GIYLTSQWTGMDSGGITVNSILSLLTIQKTKEAMSAVSQVETAWNFVFADAEGNIGYQ
                     MSGKVPKRKKGLSGFVPFCGWDKKEDWNGFESPSDLPKAYNPKEGFFTTANNDLNSYG
                     KIQPINMGMGSYRADRITDIIRKAENFTVADVCKMHYDVYSKEAEIFMKILSPILPDT
                     KQGHILKNWNFEYDIESEGAYLFEVFYKALFVEVFGKMNLGKSLVEHLQNETGVFIDF
                     YDKFVRILLSEKSIWFGSESREQIFKRVAEVSLNTEIKKWGEVQKIKFTHILFGGKLP
                     KFLGFDHGPISLPGGRATISQGQIYQAAGRTTSFAASIRIVSDMATDEVVSNMPGGPS
                     DRRFSKWYVTDLKNWISGKYKTLSANSSDIKF"
     gene            complement(8742..9326)
                     /locus_tag="PLF66_15505"
     CDS             complement(8742..9326)
                     /locus_tag="PLF66_15505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65502.1"
                     /translation="MNFPFRISFKLFAFAPQMRVTDRDKNLIFYVKQKLFKLKESVTV
                     YADETQTKELYKINADRILDFTANYYFSKVNGEKIGSVRRSGMKSIWKAHYTILDQSD
                     ETIMTIREENPWIKVLDALVGEIPFIGILMGYLLHPSYIVTLEDGSKIFRLKKKASFL
                     ERAFEIEKISDYDKNEEILLLGLMMLTLLERARG"
     gene            9882..10649
                     /locus_tag="PLF66_15510"
     CDS             9882..10649
                     /locus_tag="PLF66_15510"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HMZ65503.1"
                     /translation="MNIRAFGKTDIGVVRENNEDNFLIMNLETDAEGNVINPTSTKNG
                     ILLLVADGMGGAVAGETASLIMVTEASKYLKSHKKIKPDDAVRECLLVAHDQCHKMIK
                     LNPGLMGMGTVATVAIVSENKLYISQIGDTRLYLYRNKTLLQITEDQNFVSELVRIGI
                     ITPEQAMIHPQRNAVTQAVGSIEPILPVNYSENLEVGDKILLCSDGLNSMISDMEIQL
                     ILESSKKLDVIIDNLIYAAKDSGGHDNITVILAEVGE"
     gene            10646..11770
                     /locus_tag="PLF66_15515"
     CDS             10646..11770
                     /locus_tag="PLF66_15515"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HMZ65504.1"
                     /translation="MKSYYIDSEDVKLHVLAEDLKENKETILFLHGYPDTSSSWTKQF
                     EYFKDKFRIAAFDLRGIKNPFQDTIPTNFQMENLMQDLDSVIEFLVGSNGKVHLVGHD
                     WGAGIGWSYISDPSHLHRVFTFTAISCPHPRIFYNNAIGKLFSGNFSEIQKSLEQISK
                     SWYMILFQIPVIPELIWSTFTEPLWQKVMSVSSLPANDELLNLEKDRILDATIGNINI
                     YRGILQNGGFHLPELPQEKVTLPISVIIPENDIALSPIVYEGTKEYFPNAELQILQAN
                     HWVHREKPYHVNQIIEKFLRDFTKNSDIYFHAEWDTGNLSSGEILIQLKKKMANLNPG
                     NILKLKTKDSGLKDALFSWSLLTGNKLIRTKESEFYIKRK"
     gene            complement(11834..12007)
                     /locus_tag="PLF66_15520"
     CDS             complement(11834..12007)
                     /locus_tag="PLF66_15520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65505.1"
                     /translation="MKKVIASLLVVLIMSLGYCSSSEEKEPEAAPAEPAKTEPAKDAK
                     TPAKTPAKTPAKK"
     gene            complement(12108..13289)
                     /locus_tag="PLF66_15525"
     CDS             complement(12108..13289)
                     /locus_tag="PLF66_15525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010577867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HMZ65506.1"
                     /translation="MKMNLISEERQKELIKYRRTIHKNPELKYEEEDTALFVKDHLNR
                     LGLSFQDKIAKTGIVSLIDSGIPGKTVLVRADMDALPIIEENNTEYVSQKKGVMHACG
                     HDAHVSVLMGFAEEIKQDIKSILPSGRVLLVFQPAEEGGSGADKMIEEGILEKYKVDA
                     AFALHVWNHVDLGKVGVVNGTMMASVDEFKIIVKGVSGHGAMPQHTVDPIVVSAHIIT
                     ALQTVVSRSVDPLEPCVVTIGSIHAGNAFNVIPETAELTGTVRTYSKKVYEEVPEKLK
                     KIVNGIADGFGATVEIIYKRVDKPTINDPAMADVVRKAAYKLLGNDSVTEEEVRTMGG
                     EDFSAFLLKVPGCYFFIGSRNKEKGFVNPHHSSKFDFDEEAMGIGLAMIKEIVSSYLS
                     T"
     gene            complement(13519..15090)
                     /locus_tag="PLF66_15530"
     CDS             complement(13519..15090)
                     /locus_tag="PLF66_15530"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002178479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HMZ65507.1"
                     /translation="MNTPKPIVNLIDYSKQPFNLAIATARTCYSSKGILLPEDMVKSE
                     KSIEIRDRVAKSTKKAGHLTTRQHPQFIFTIDKVSRQFVWSFLHSHPFYNSEQVSQRY
                     VEVKKENYYIPQNLNGKELELYLDAVNYSSDSYFDFIDRLNPYIGKEYFSVYRARANY
                     PEKWQDPIKKKSLEIARYLLPLATYTYLFHTINGLTLHRYHRLMNTYDVPEEQRIVVE
                     QMIEAVKSVDPLYVEEMDDPIPLEETSEYQFFEKFYSSKSAYNPSYTKEFIEEFDSDM
                     EGRYSKLVGYNENSPAILASAVRSVLSIPKSKLSDEEAIRLVLSPAHNHHLTSTLNET
                     TMSPISRSLSQVHYTFKKRISHTADSQDQRHRMVPGARPVLLSQYAGKPDYITPAVIE
                     KYPELKEEYEKRMNGIFERVNRFLNECKKEEYATYLFPNAIPIRFYESGDLLNLHHKW
                     RSRLCYNAQEEIFYASLDEVKDIQKINPEISKWIKAPCGIRQMGEQKPYCPEGDKYCG
                     VQVWKQELENYKRVI"
     gene            15321..16115
                     /locus_tag="PLF66_15535"
     CDS             15321..16115
                     /locus_tag="PLF66_15535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012387293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65508.1"
                     /translation="MKKTETELKEIIQNAVNKNNILSMKTYLLSDYGESILKIISSTI
                     LEKFNRMDLMDIAYSSAKELVINATKANLKRVLYKEMGLDINNPDDYQKGLNEFKGSL
                     TEDKIATYKDLFKKHDLPVTATFYYTKDVLNIKIKNNFPLLPIEEERIRDKFQKATSF
                     SSLIDFFMEYGDNTEGAGMGITMVGILLDQSGINKHSFSLYSSDKYKETVAKIEIPLH
                     EGYLSKREVFEKELREKGITPEEFRKNFSYNYKHFDKMKSDEVKTN"
     gene            16208..18742
                     /locus_tag="PLF66_15540"
     CDS             16208..18742
                     /locus_tag="PLF66_15540"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HMZ65509.1"
                     /translation="MHRKLFVFTILFQTILFTQKINAQQVTPSKNSKKNQTDLEIQRQ
                     SLEIERERLTKEQNEFSKLKESFLKEKESLEKEKEELQKQKEELDQQKTNKAKDSTEE
                     DSKEEDFFKLEESISIVSKRVGESEKTSKTSAIVSVYNRQTISDMGARNLADILKQVP
                     GIEILYDQFGSYKVAFRGVLSRSGVLMLLDGHRINNFYDGSTFLDIRADAIEKVEIIR
                     GPGSSIHGTNAFVGVINVITRSSSGRNGNGYVASRLGQYNTIEPTAFYNIKMGENWNL
                     GTYAGYYESKRPEVHLPNDQSCGRTLYRQGICTSVIPLPLADYIKTNDRKKQTNLFLN
                     LNRGDDFYIKGKMIREDRGPNVGELGYLTPNSELTFNLMTLDIGTQKIAITEKLSLSA
                     RLYGDTFLKKDQIQVERPDMINHIGMSPIKKTGYKYDTKGTEVILQYNPLSQLSIMLG
                     AQLEKLTYKDAYVQKNFAGNNTSNLYPFFYDFDNLDRDRNDTILAYFTTGKAFVSDPE
                     SLQKGKTKSRNIQAEFLQVLWDPFKWLSITMGVRRDVYSDFGSTINPKSGFVLTPFEK
                     TRYGSLSFKFLLGSAFRAPTFKEMYDRSQTYQFGGVLGNNTERFQNYGQAGYGPLKPE
                     TIRTGEVGMEYQTPYKPLNIQANWYYTRIYNNIDGVNVSSTPPGKLDIYHNLRGLTIL
                     GTEVEAKLSFSKRNYIFANVSWFQAVDHGGLNGIVTSDTKTFLTSIPQARANLGVNWN
                     LFKYFILNNTIWASSERQSNARFAFERLSTKGFQYSQYYIWNVSLGTTEELNENLEFR
                     FSIFNLNNYKLYDHSNTATEDYNNRAIPAAYIYQRYIEFKVTYFIK"
     gene            18752..19747
                     /locus_tag="PLF66_15545"
     CDS             18752..19747
                     /locus_tag="PLF66_15545"
                     /inference="COORDINATES: protein motif:HMM:NF014871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HMZ65510.1"
                     /translation="MKSYILLFLLLSSLFCTQKQNPFDDRSALVSVVNPLSSGTPKSF
                     GLDLKIDSRLNRGCETRIGNLMADVFADKGNSILGFYNGGAIREDFGLQSIYPNSIIP
                     KGTIPNSDLLKKLFPLTGGNLVRINLNTYRLKQALEVSVGRLNARAERGTDDIDSDGP
                     THGNCWLNPNLSGAGRFLHVSSKMQIEVNPVATAVVIGGSGTTNTLVITSEGRRIIKI
                     IIDGILLYNNSTGDITSGWSAGQSSCTIKGITYSNSAACNFFSATIPKFQFDGGDSNA
                     TLNPNMLEVNNDGSVVVSQTGIGANLDDGTILNDYLQKFTTGPVYPRISGRILMP"
     gene            complement(19754..21451)
                     /locus_tag="PLF66_15550"
     CDS             complement(19754..21451)
                     /locus_tag="PLF66_15550"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HMZ65511.1"
                     /translation="MKKIPLISALFRDSKSIRSKLTLTISFVVILLLTISSIFFTGML
                     LSELRRSLVKRGIMITRSMGLVSENLIASLNFSEVERGAAELVENDREIVYVILESQE
                     GNSIVERDKTLLGSDGERTKDKVISEIKAEEETGQNMEIRQIEHNGLECIDVLYHVKA
                     GDRELATLRIGLTTRYLNLSLYKSVFISVLLIIAFSLMGIFISIRFSLHFSTPILNLK
                     NATEEMQNKNFDYKIEVLSNDEIGLLADAFDEMRMSIKTYSSSLRELNLNLEKKVEER
                     TRELQNVLHEVNILHEQKKGDYYLTANLLHPLIGSFIENPHVKIDYTLNQKMKFEFRN
                     SASQIGGDFILADTLRLGDKNYCFLCNADAMGKSMQGAGGALIFGSVIRSILNRTQYT
                     EISGQITRPEEWLEDCYIELVKVFYSMQGTMSISCILALLDDETGRMHALNAGHPPIV
                     LFRDGKASFLGENDVNQKIGESFVYEKIRNNNLKISHISLNHQDVLFFGSDGKDDVLL
                     KGNDSGKREINSSDQFFLACLEEAKGDIYKVESVIQNKASLMDDYSLLRIEFTNSKI"
     gene            21612..22385
                     /locus_tag="PLF66_15555"
     CDS             21612..22385
                     /locus_tag="PLF66_15555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65512.1"
                     /translation="MKRLFEIILLFCVQFSVFAEEFTIVYFDPDGNLRNITSLQSEGN
                     KYFQSISPDINFIVIASEQILSTELEKLKPQIMIVQSLYYTRQKNSLGFKPFYIFLAK
                     DNHLYFKKIITMNPDFKTLEDLDKKIVASALPKDTTKELTGGERNWKILTVPKDMDAL
                     MAIKFRQADAALVAESSLDNFKKLDPTDAQNFNSIFTTKSIFQPLVVTTKYATNKDLI
                     PKIMDSMKKIKADTNGQNFLRMLNFDDISDNKKVLGSVP"
     gene            22382..23323
                     /locus_tag="PLF66_15560"
     CDS             22382..23323
                     /locus_tag="PLF66_15560"
                     /inference="COORDINATES: protein motif:HMM:NF016292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HMZ65513.1"
                     /translation="MRFISYLIALLLLINSCQSPNRILEGMKFQGNKKKIFILVSEQI
                     SSFEVVKNSFMEEGKKEYQFLSLNLDGNYDDLEQLPAQIRKLKPDLILCIGSKSLESL
                     AGKITEIPILFAMVLDYQRFHPEKYGNIAGVSLSIPPETVFYNFKNYFGEINQYGVLS
                     SRDHISSLRENRERLKNVNIQLTIGETNSPDDVQNKYDAIKKSIQALWMVPDPKVISE
                     ESFLFLVDNTINDKKSFIVYSEQFVKAGALFSTSPNYSTIGSQLAIKSQQILEDRQKP
                     VQVGISPIIGTFLTLNKTTMKKLGLNVRMDGIDQVFE"
     gene            23419..23703
                     /gene="gatC"
                     /locus_tag="PLF66_15565"
     CDS             23419..23703
                     /gene="gatC"
                     /locus_tag="PLF66_15565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002974391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HMZ65514.1"
                     /translation="MEKEKLQAIANLAKLRIEDSQLDSMVNDFNKIMAYVDSVKELNT
                     ESVSEEEIYEWSENKVRPDILGKSLTRDEIAKISPKFENGYIVVPRVIET"
     gene            23700..25157
                     /gene="gatA"
                     /locus_tag="PLF66_15570"
     CDS             23700..25157
                     /gene="gatA"
                     /locus_tag="PLF66_15570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016548584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HMZ65515.1"
                     /translation="MIFKKYAEIKKDLQENKITSVDLCKAYLKRIEENDSKIKAFLYL
                     NKDRILQEAASSDERRKAGKALSDMDGIPIGIKDNICIKGERVSCGSRILENFVSPYD
                     ATAIQKLRDKGFIFFPGLNMDEFAMGSSTENSAYQVTTNPFDTTRIPGGSSGGSAAAV
                     AASMLPVALGSDTGGSIRQPASLCGVFGLKPTYGRISRYGLVAYASSLDQIGPLSNDP
                     QGIIDVLSVLSGSDLNDSTTSPEKPFTVSDLTKIDLKNTKIGIMANEGAEWEKDVKDR
                     FHQIIDILKSKGAKIIELDFSIHSYAIPIYYIIATAECSSNLSRFDGIRYGYRVEDPK
                     KLEDVYIESRSKGFGSEVKRRILLGTFSLSSGYYDAYYGQAQKARVLIKKSYAEYFKQ
                     VDFILQPTSPTTAFKIGEKTKDPVQMYKADILTTSVNLAGLPAINIPAGVDNKGLPIG
                     LQITANKFFEGKLLSFASEVSSLDECKVKLPENIQ"
     gene            25154..25921
                     /gene="hisF"
                     /locus_tag="PLF66_15575"
     CDS             25154..25921
                     /gene="hisF"
                     /locus_tag="PLF66_15575"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HMZ65516.1"
                     /translation="MTELTKRVIPCLDVKGGRVVKGIQFVDLRDAGDPVECAISYEEH
                     KADELCFLDITASSDRREILLHLVESVASKIFIPFTVGGGINSVAMVREVLVRGADKV
                     SINTAAFENSKLLKDASEIFGSQCIVCAIDAKYHSERKRHEVFLHGGRTETGREVLDW
                     ASEAEEMGAGEILLTSMDRDGTKDGFDIPLLKQITTNLRIPVIASGGAGNAEHLVEAI
                     LRGGADAVLAASIFHFKEQSIIEVKQSMKEMGIPVRL"
     gene            25918..27672
                     /locus_tag="PLF66_15580"
     CDS             25918..27672
                     /locus_tag="PLF66_15580"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+:solute symporter"
                     /protein_id="HMZ65517.1"
                     /translation="MSLFTWIDIFIIFIFLFFSLFLPYLFSKKDSTLKGYFQASGRMP
                     WFISGTAMVATTFAADTPLAVTELVAKFGISGNWLWWYGSISSIATVYFFAPLWKKSG
                     VMTDIELVNLRYSGIGAEILRIFKSIYLGFFMNILILAWVNLAMVKVVEVLLPDVSAK
                     LIVSSLFLFAFLYTIYMGLNGISIADTFQFFFAMIGCVVLAYFVLSIPEVGGIDGLQQ
                     KLPPSYFNFIPDFQNPASKDFSVESFLLFIGVIWWSSWYPGAEPGGGGYIAQRILAAK
                     DVESSLLASLWFTIAHYFIRPWPWILVALASVVLFPDLSLEDKGKGYVLIMTKENLPV
                     GLLGLVMAVFMAAYLSTIATHLNWGSSYLINDLYKPYLAKEKSDKHHLAAGKGFQLLM
                     MAFSLLISFYWIKTISGVWQFLLECGAGVGIVLILRWYIPRLNAWSELTGFIAPFVFY
                     SISKFILHWESPTSVLFTVFCVLITILFITFLTPKVDLENLKSFYQKVNPPGLFWAKW
                     AKENNLEWKPSELNLLHSLLLSATGILLIFSGLFSLGNIILKRYDSLVLSISLFIFAI
                     VLTYHLFPRHLDLENKKE"
     gene            27734..27805
                     /locus_tag="PLF66_15585"
     tRNA            27734..27805
                     /locus_tag="PLF66_15585"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27767..27769,aa:Cys,seq:gca)
BASE COUNT         9474 a         4910 c         4627 g         9032 t
ORIGIN      
        1 cgatgttgta aagatgagag aagtcttatc gatagtcccc tcctttgaat cggaaaaaga
       61 aattgtttga atagaaactg tatccgtcgg agctttgttt aaagagattc gaatttgaag
      121 actagtccca gattcacttg tttgaagtcc agttgatggt gagattacaa attgtgcagg
      181 tgcggatgtt ggttttattg gaaagattcc aaagattttt ggaaatgcac taccggcctt
      241 acaaccaagt agacagcaat aaatcgctgc aaacagaaat gaacccatcg ataatttcat
      301 acggggtaaa cttatacaca ctaattaaaa attgtttttt ttgcaaatct aatttaagta
      361 gaagttctaa attctttata ggacattact gattttttac tagagtttaa tcgggtttaa
      421 aatcagaata aactaaaaca cattctttac tggattcgaa attgcgggaa agagtctgga
      481 tccaatttct ctaatgaatc actctctata tgtaatcaga aattaaccca agtagatggc
      541 taaatgcaag tcgagccatt tacaattgtt tttatttctc tccttcctaa aattcacgca
      601 atctctcctg tttcatctat tagaatgcaa aaagcaaatg attaaaatgg tagattttta
      661 cgaattggaa taaactttac caatatgata aaaatttgga ttcccttttt gattcttttt
      721 tctacaaact tacttaaagc aaaggaaacg atttctgcaa agtcttatct aatcgaggat
      781 cgagatacaa aggtaatcct atctaaaaaa aacacatcga tagttttgcc aatggcaagt
      841 atcacaaaac ttgcaaccgc actactcaca cttaaaaata aagatttaaa tgattttgtt
      901 aaaatcacaa aaacaacagt tctaagaaat gaaacggata agagcgcttt tttaactgaa
      961 ggagaattct atacgcgaaa agatttactt ctaactatgc tagtctcctc taccaatgtt
     1021 gcagccattt cactagcagc taacgtttct ggaagtcctg aaaaatttgt agaagattta
     1081 aacttatggg caaaagagaa tcaattagag aaaacctcct ttggagatac atttggactt
     1141 tcccaaaact ctaaatccaa tatagatgat attgcaaaaa ttttagattt aatcgaatct
     1201 aataaagatt tatttacaat actcaccaca actagttata caattacaga gaaaaaagga
     1261 aaaaaacaaa atcttacaac aacaaatgaa atgccaacct ataaagagta taagatattt
     1321 ggaaaaacag gaactacaaa tgcagccggt aaatgctttg ctggattcat tacgaaagat
     1381 tccaaaaaat ggaaaatcat tttgctcgga tctaaaaatg ttttttcaga tatgaaaatt
     1441 ctaatagatc aaattcaata atataggtta cataaatagg atgcagtttt ccaatctaga
     1501 ttttttagtt ttaatttcct accttgctat cgttttactc gcaggacttc atagttccaa
     1561 aaatgataca tctaaaagtt tttttctagc tgataaagaa ttgccttggt attttatcat
     1621 gctctcgatt gtggcaaccg aaacgtctag tcttacgttt ttaaacatac cgggattgag
     1681 ttttaaagga gattttagtt ttttacaagt agccttcggc tatattatag gacgagcttt
     1741 tgtggctaac ttcattttac ctctctacta taaaggtggt tactcttctg tttatgaatg
     1801 ggtaggcgaa aaattcggaa aagaaactca aaaatcagtt tctagtgtat ttttaatcac
     1861 tcgtgtttta ggagatggaa tacgtcttta tgcaacgtca attcctatcg caattatatt
     1921 tcatggctat tttcagaatt tagtttctga agagacaact gggattctta ccttatttat
     1981 aatcacaatg attacaactc tctatactgt cttcggtggt tttaagtcgg tagttgtgac
     2041 agatagtttg cagtttttta tttatattgg aggtggagtt ttttctttcg tatacctaat
     2101 gcaagatcta ggcaatcata tttctttttc tgaaattctt tctacaagtt tatcaaatgg
     2161 caaattaaat ttctatcaag gctcaacagg aattacaact tccaattttt ttcaaaaacc
     2221 gtattttttt atcaatggtc tcataggagg aattttgtta tccattggca cccatggagt
     2281 agatcaaatg tttgcacaaa gactcattgc ctgcaaatca gaaaaagaaa gcaagctagc
     2341 gcttatcggt tctggaattt tagttttctt acaatttgtt ctatttttag gaataggact
     2401 tttacttttt tacaaatacc aaggaagtga gattcaccca gacaaagttt tctctaagta
     2461 catcattgaa aatatcccaa caccaatcct tggaatcata gttgccgcga ttctcgctag
     2521 tgccatgtct actctatcta gttctatcaa ttctatgtcg ttatccgttt tatttgattg
     2581 gatgaaaaaa aacgaaagtg aacatcaaag agttttactg tctaaatgga ttagtgttct
     2641 ttggggcttt gttttatttg gaagttctct actcccgtat tatttctctg gaaaaacttc
     2701 cgaaggctta gttgaaattg gtttaaagat tgcctctttt acgtttggac cactcactgc
     2761 actctttcta ctcgttcgat tgaaagatag atttgctcta aatctttacc catctctttt
     2821 aattttttct ttgtttctaa gcttagtcac cactatttta tttagcattt cacttaaacc
     2881 agccatggca tacctcattc cttttggtat ttttattttt tatctgacaa tctttacagg
     2941 gcaattttta aaaccaaaag tttaatttgt aaatgataca acaattttcc ttttgttaca
     3001 ttgtccctaa actcaatcca aaagtcgcag cataatgtgg tgaaaatttt tttatgaatc
     3061 gagttctgtt cacacaaaac ttataaatgc gaatcagtag taatctttca aaaccgactc
     3121 ttagcaaatt aaatctatcc aaagttcgat gataaaaaga agtaatcctt taaaattctg
     3181 attgacatac tttttactca gcatatcgtc ttggcgtttt tcaccaaaca gggattggtt
     3241 ccaaaatgat tcatatcaaa atcatgctag tttttctaat acttagcctg agccattgct
     3301 tgggtatcaa aagttctcag gaaaagccta aagtaactag caaagaaggt atactcgacc
     3361 taagaaattg ggatttttac aaatatggaa tggtcgaatt aaatggggac tgggaatact
     3421 atcctgaaaa atttgattta ggtgaaaaca caaacgagaa aaaaccaaaa tctttctacg
     3481 aatttggttc caaaataaat ccgaatcaag cctacccaga atatgcaagc ttccgattaa
     3541 aaatactatt acctgaaaat aaacccgaac ttcttttctt taggatagca ggattaaata
     3601 gttcttataa tttatatgct aattttaaat taatagcatt tcgtggaatt gccggcaaat
     3661 cgaaattaac cacaactcct tccattggtt ctgatatttc tcagggaatc ttagattcta
     3721 aagaaatcaa tttaatttta caagtctcag acttttattc taataaacat ggatttagcg
     3781 gcaaattaga gttaggcaca atgtctgaat taggatcgag tgcaaatcta gaattgctaa
     3841 caagctttgg acttgttttg tttttatctt ttgtattttt gattagtatt actcaaatat
     3901 ctgaaagaat atttttatta ttgagtattt cttctattgg acttattttt catattagtc
     3961 tttcttaccc atatccaatc agagtcattg catctaagac aagagaagat tttatttact
     4021 atttcagaat tttcaatcaa ggtcttgtta caatatgctt tgcttatttt tattatcaaa
     4081 tttgcgaaga gttaaaaagc atctgggcaa agatatccat tacaggagcc ttttctgttt
     4141 ttattttatt tctttttctt tcatcgaatt acattcctcc gaaattagaa atcatatcta
     4201 gtttttattt agcatttact caaatcgttt ttacctatca ttttttccta gacggaccaa
     4261 gttcctttgc acgaatcaaa gcatatacaa agaaatacaa agcagaatta gatagaacgt
     4321 taggagaatt aactgatagt aaaaaagact ttgataaaaa aattattgaa cgttcattca
     4381 atatggtcac ctatatggag aaattagaga tccagaccgg tgagatggag aaattaaatg
     4441 aactgattat tcacttattg gaaggagcaa atataaattc tgttctagat gagattttct
     4501 ctcatatcat gacttactat agatccaaca ttgctttttt atatttccta gatcctgtta
     4561 caaatgaatt ttttccctat agaggaatca caaaggatat tccagaagag ataagatctt
     4621 ttatggaaaa ttctagaatg ccagtttcta gagaagctgg agttgcctat attgcttata
     4681 aaagaaagaa aacaatttat cttgaaaatg ctaaaacaaa atatactaga aagagagaaa
     4741 aaggaaaagg aattccagaa ttagtttctt ctctttacat tccagtctta attcgaaatg
     4801 aattccaagg aatctttttt ctagctacct ttaatcatcc tctcaattta aacaaagaaa
     4861 aaattaagtt tatttctatg tttacaaacc aactaacggc tgcgattcaa aaagaaaaac
     4921 ttttaaaaga aatggaagaa gaaaaaagaa aagcggaaat ggctcgtgag tttgcggaaa
     4981 ttgcaaaaaa agaagcagaa gaggcaaaag aagaaattga agcaatcaat agcttaacaa
     5041 aatcaattaa cgaaaactta gagttaaaat atataatgga taagattatg aactttgtaa
     5101 aagatcatta tggaattcat tactattctc ttcatattct aaatgacaat aaaaagaaaa
     5161 tgaaactact agaagcaaaa tttccagacc atgtaacatc agaagaaaaa agaagagttt
     5221 ctcaaattca tattcctgtg caagatagtg aaggtgcttt tccatttatt tttaaaacca
     5281 agaaaatgat ttactataaa aaattaaaca ttgataaatc aaatgaagaa gaaagagagt
     5341 cgcttagaaa tttacatatc tcttctatgg ttggaattcc tctcaaagta aaaaatgaaa
     5401 tcattggtat tttaaatttc tttagtccag aggaattaag tcttacaaaa acacatttaa
     5461 aaaccattgc caatctagga gaacaagttg cgggagtcat tcataattct agtctttata
     5521 aagatatcaa agtagagaaa gacaaatcag aggggctact tttgagtatc ctccctccta
     5581 aaatagcaac agaactgaaa aacacaagta aagtagttcc aattatttat gaatcggtta
     5641 ctattttatt cacagacttt gtaggtttta caaaaattgc tgaaacactc ctaccaaaac
     5701 aactattaat cgaattagat ggatatttta ctttttttga ctttgtatgt gaaaaataca
     5761 atatcgagaa acttaaaaca atcggtgact cttacatgtg tgccggtggt cttccaactg
     5821 caaataaaac tcatgcgata gacgcttgct tgacagcgat tgaatttaga gattttgcaa
     5881 ttagaatgca agaattaaat gctacctcca aggatgaaat gatgccatgg gaacttagaa
     5941 ttggtttgca tacaggtcct gtgattggtg gaattgtagg aactactaaa tttgcttatg
     6001 atgtttgggg ggatagtgta aatattgcaa gtagaattga atcttctagt gtcccaggaa
     6061 gaattaatat ttctgagact acatacgaat tggtaaaaga tttttttatt tgtgaacata
     6121 gaggaaaggt aagcgcaaaa aacaaaggag aaattcgaat gtatttctta aaccgattga
     6181 aaccagaatt ctccgcaaat gaagagggca cagcacctaa ccaaaaattc tacgaatact
     6241 atgaaaaaat caaactcggt caaccaattc caatccaatc tagtagcaaa ctaagactag
     6301 tcgtttaacg tcagtttgat aaaagaaaat ttaaaagatt accactgatt aacaacgata
     6361 aaaaacgatg tgtatacata aattatcata tacattatcg aatgcgtaaa caagagaagg
     6421 cgcgggtcgc gaatactcgg agcgaagggt ggaatataga caccgataaa ataaaaaaaa
     6481 aataaaccca aactcaatgt tgcttcctag ggtcacaaca taatgtgttc atccgtggta
     6541 aaacattctt acaatcaagt tcacgttatt tggatatagt atccttttaa tcttaaaatt
     6601 ttatatcact cgaattagct gaaagagttt tgtattttcc actaatccaa ttttttaaat
     6661 ccgtgacata ccatttagaa aagcgcctat cggatggacc accaggcata ttacttacaa
     6721 cctcatctgt tgccatatct gatacaattc gaatggaagc agcaaaactt gtagttctac
     6781 ctgcagcctg gtagatctgc ccttgagata ttgtagctct tcctcctgga agagaaattg
     6841 gaccatgatc aaaacctaaa aacttaggaa gttttcctcc aaataaaata tgtgtaaatt
     6901 tgattttttg aacttctccc cattttttta tttctgtatt aagacttacc tctgctactc
     6961 gtttaaagat ttgctctcta gattcacttc caaaccaaat agatttttca gatagcaaga
     7021 ttctcacaaa tttatcataa aaatcaataa acacacctgt ctcattttgt aagtgttcca
     7081 ctaacgactt tcctaaattc atttttccaa acacttcgac aaagagtgct ttataaaaaa
     7141 cttcaaatag ataagctcct tcagactcta tatcatattc aaaattccag ttctttagaa
     7201 tatgtccttg tttagtatca ggtaatatag gagataatat tttcataaat atttctgctt
     7261 cttttgaata gacatcataa tgcatcttgc agacatctgc cacagtaaaa ttttcagctt
     7321 tgcgaataat atctgttata cgatcagctc gataagatcc catccccata ttgatcggct
     7381 gtattttccc ataactattt aggtcattgt ttgcagttgt aaaaaatcct tctttgggat
     7441 tataagcttt tggcaaatca cttggggact caaacccgtt ccagtcctct tttttatccc
     7501 atccacaaaa aggaacaaaa ccactcagac ctttttttct tttaggaact tttccagaca
     7561 tttggtatcc tatatttccc tctgcatccg caaatacaaa gttccaagca gtctctacct
     7621 gactgactgc actcatagct tcttttgtct tttgaatcgt caaaagagat aaaatagaat
     7681 taaccgttat tccaccagaa tccattccag tccattgaga cgtcaggtaa atcccttcct
     7741 taaacggatc accggataaa gttccatgtt cattttcata gaatataacc tcttctgttt
     7801 tcttcttttt tcttttaata gattcctttc gaattataaa gggtgtatat tttccttctt
     7861 ttaaatactt tccttcttta cagtcttcca tccaagaatc ataagcatct ataaatgcgt
     7921 atgtaactcc ccaactcaaa tgatttgttc ttcctgtcaa aattccaggg actccgggca
     7981 tactgccacc tatcgcatac caatcagaag tcgataaaat cacctctccc caaacagaag
     8041 gcaatcggtt aatctctaaa tgaggatcat tggctaatat tggtttgcca ctctttgttt
     8101 ttttccctcc aatgacccaa ttatttgaag ccatcatttt aggcgcaccc atttcccaaa
     8161 gtgaagaagg aacaactcta tcttccaatt tgattttttt aattgaatca aattttgctc
     8221 catccaaaat atttggaaaa agttccttta aatgattttc actgagacca gcttgaacca
     8281 tttcaataaa aagtctttca atctctgctt gcgactgggc taaagtcaaa taaccaatca
     8341 ttctagatat taaaatggaa tcttctatcg tccaagcttc tttaggaaca ccgagtaatt
     8401 taaactcaaa cgggaaggat tctgcaaaac catcgtttac tccatcacaa taagacttga
     8461 gccattgctt cttttcctta tccacttcgg ctaacggttt tttagttcta ccataccaat
     8521 tcattcttct aaaaaattta tctatctcaa gagaatgatc actcgaatct aacagttctg
     8581 aaagcctacc ttgccctaga attttcataa agaccatctg cattcctcta tcaacagcat
     8641 gcacataacc taatccatag agacattcct ctattgtatt tgcttttatt tttggtgttc
     8701 ctgaatttgt tcgagtaatt ttaacgttag tattcatttc tttaacccct tgctctttcc
     8761 aataaagtga gcatcataag tccgagtaat agaatttctt catttttatc atagtctgat
     8821 attttttcta tttcaaatgc tctttctaga aaggacgctt tcttttttaa tcgaaagatt
     8881 ttagaaccat cttcgagtgt aactatatag gagggatgta ataaataacc catcagaatt
     8941 ccgataaaag gaatttctcc cactagtgca tccaaaactt taatccaagg attctcttct
     9001 cggatcgtca taatcgtttc atctgattga tctaaaatag tataatgggc tttccagata
     9061 gacttcattc cagagcgcct aacggatcct atcttttccc catttacttt ggaaaaataa
     9121 taatttgctg taaaatctaa aattctatct gcatttatct tatataattc ttttgtttgt
     9181 gtttcatctg catagacggt gacggactct tttaacttaa atagtttttg cttcacataa
     9241 aatattaaat tcttatctct atctgtaact cgcatctgtg gagcaaatgc aaagagttta
     9301 aaagaaattc taaatggaaa attcataaat aagtccttat ggttttacac tcattctttt
     9361 ttttcaatta accttgtcag tatttttatt cgctttcctt tagagttcag atttaacttt
     9421 aaattgactc attgatttca aaagtgagtc tctgaattta attggatcta atagcgcata
     9481 gaaaggatat tttagcgatt caatcgtatg aacctatttt tacggaaaaa catttccaaa
     9541 tcataaccga ttcttttttt taaaatttac aattcagaaa tagactaatt tttgcaatga
     9601 attgaatcaa agtagacgtt aaaccaaaga atcgaggctt tttctaccat tttataggct
     9661 aaaaagaaac tagagtttaa atatcgattt ctcaaattaa gaagatttcc aaaagaaaaa
     9721 actctttgta ataagaattc taagttattc cttaaagatt tattgagaat ccacctagca
     9781 ccgtatttga atcgactctc caatcccttt tcaataattt attccttaaa accataaaaa
     9841 tgttgtccta aatgaattag aatcaattat aacttaaaca tatgaacata agggcttttg
     9901 gaaagacaga tattggtgtc gtcagggaaa acaatgaaga taatttcctg attatgaact
     9961 tggaaacaga tgcagaagga aatgtaataa atccaacctc aacaaaaaat ggaattctac
    10021 tacttgtagc agatggaatg ggtggagcag tcgctggtga aaccgcttct ttaatcatgg
    10081 taacagaagc ctctaaatac ttaaaaagcc ataaaaagat taagccagat gatgctgtta
    10141 gagaatgtct tttggttgca catgatcaat gccataaaat gataaaatta aatcctggct
    10201 taatgggaat ggggacagtt gccacagttg caattgtttc cgaaaataaa ctctatattt
    10261 ctcaaatagg agacactcgt ctctatctct atcgaaataa aaccctactc caaattacag
    10321 aagatcaaaa cttcgtttct gaattagttc ggattggaat cattactcca gaacaggcaa
    10381 tgattcatcc tcagagaaat gcagttacac aggcagtcgg atccattgaa ccaatccttc
    10441 cagtcaatta ttccgaaaac ttagaagtgg gcgataaaat tttactctgc agtgatgggc
    10501 ttaattccat gattagtgat atggaaatac aactgattct agaaagtagc aaaaaattag
    10561 atgtgattat cgataattta atctatgcag ctaaggatag cggtggtcac gataatatca
    10621 ctgtaattct agctgaggta ggagaatgaa gtcctattat attgactcag aagacgttaa
    10681 gctacatgtt ctcgctgagg atttaaagga aaacaaagaa actattttat ttttacatgg
    10741 ttatcctgac acatcgagca gttggacgaa acaattcgaa tattttaaag ataaatttag
    10801 aattgcagcc tttgatttaa gaggaatcaa aaatccattt caagacacaa ttccaactaa
    10861 ctttcaaatg gaaaatctaa tgcaggattt agattctgtt attgaatttt tagttggctc
    10921 caatggaaaa gttcatttag tcggacatga ctggggagct ggaatcggtt ggtcttatat
    10981 ttctgatcct agtcatttac atagagtctt tacctttact gcaattagtt gtccccaccc
    11041 tagaattttc tataacaatg caattggaaa actattcagt ggaaattttt ctgaaattca
    11101 aaaatcatta gagcaaattt caaaatcttg gtatatgatt ctatttcaga ttccagtgat
    11161 tcctgagctt atatggtcta ctttcacaga gccgctctgg cagaaagtca tgagtgtatc
    11221 tagtctaccc gctaacgatg aattattaaa tttagaaaaa gatagaattt tagatgcaac
    11281 cattggaaat ataaatatat accgaggaat cctacagaat ggagggttcc atctcccaga
    11341 actcccccaa gaaaaagtaa ctctaccaat ctctgtaatc attcctgaaa atgacattgc
    11401 tctatctcct atcgtttatg agggcacgaa agagtatttt ccaaatgcag aattgcaaat
    11461 tctacaagcc aatcattggg ttcatagaga gaaaccttat catgtaaacc aaatcattga
    11521 gaaattctta cgagatttca ctaaaaattc agatatttat ttccatgctg aatgggatac
    11581 tggaaatcta agttccggtg aaatcctaat tcaattaaag aaaaaaatgg caaatttgaa
    11641 tccaggaaat attctaaagt taaaaacaaa ggattcaggc ttgaaagatg ctttgttttc
    11701 ttggtctctt ctgacaggaa ataaattaat tagaacgaaa gaatcagagt tttatattaa
    11761 gaggaagtaa aaaaaaaggc ggataatttt ttcaaatcat ccgcgctctt ttcaatctat
    11821 tttttaagtt agattatttt ttagctggtg ttttagctgg tgttttagct ggtgttttag
    11881 catctttagc aggctcagtt ttagctggct cagctggtgc agcttcaggt tctttttctt
    11941 cagaagaaga gcaatatcct aagctcatga ttaaaactac tagtaatgaa gcgataactt
    12001 ttttcattaa atatctcctt aatggtttaa tggtaatatt ttttatatag gctttaaaat
    12061 caaccttatt ttggaaccaa agtaagattt tattttacaa atatttttta ggtgcttaaa
    12121 taagaagaaa caatttcttt tatcattgca agcccaattc ccatagcctc ttcatcgaaa
    12181 tcaaatttag aactatgatg aggatttaca aatcctttct ctttgtttct agaccctata
    12241 aaaaaataac aacctggaac ctttaataaa aaggctgaaa aatcttctcc tcccatagtt
    12301 ctcacttctt cttctgtcac agaatcattt ccaagaagtt tataggcagc ttttctaacg
    12361 acgtccgcca tcgctggatc atttatagta ggcttatcta ctcttttata gataatttca
    12421 accgttgccc caaagccatc cgcaattcca tttacaattt tttttaattt ttctggaact
    12481 tcctcgtaaa cttttttaga ataggttcga accgttccag tcaactctgc cgtttcagga
    12541 attacattaa aagcatttcc tgcatgaata cttcctatcg ttaccacaca gggttcaagt
    12601 ggatcaacag atctggaaac aacggtttga agagcagtga taatatgagc agacacaaca
    12661 atcggatcta ctgtatgttg cggcattgcc ccatgcccac ttacaccttt tacaatgatt
    12721 ttaaattcat ccacggatgc catcatcgtt ccattcacaa cgcctacttt ccctaaatcc
    12781 acatgattcc aaacatgaag tgcaaaagca gcatccactt tgtatttttc aagaatgcct
    12841 tcttcaatca ttttatccgc cccactccct ccttcttctg ccggttgaaa aaccaataga
    12901 actctaccac tcggaagaat tgatttaata tcttgtttta tttcttctgc aaaccccatg
    12961 agaacagaaa catgagcgtc atgtccacaa gcatgcatga cgcctttttt ttgcgacaca
    13021 tactctgtat tattttcttc tatgattgga agcgcgtcca tatctgctcg aactaaaact
    13081 gtctttcctg gaattccaga atctatgaga gatacaattc cagtttttgc aattttatct
    13141 tgaaaagaca agccgagtcg atttaaatga tctttcacaa aaagcgcagt atcttcttct
    13201 tcatatttta actctggatt tttatgaatc gttcttctat attttattaa ttctttttgt
    13261 ctttcttctg atattaaatt cattttcatc tcctactcta cctatttatt aaagctacta
    13321 aagccttcct attatctgaa ttatcaccta acgcttttaa cttcaggatg tcacttagga
    13381 caccggttta ataataaaaa acatttctaa atgatccaat tccttatctg tgcttagaaa
    13441 acaaccggtc ttttcttgaa agaatctttt tcttcttttc taattcgaga cgattttgtg
    13501 aatacttttg ctctacaact aaatcactct tttataattt tctaattcct gtttccagac
    13561 ttgcacaccg cagtatttat ctccttcagg acaatagggt ttttgttccc ccatttggcg
    13621 aattccgcaa ggagctttaa tccatttact gatttcagga tttatttttt gaatgtcttt
    13681 tacttcatct aaagatgcat aaaaaatttc ttcttgtgca ttgtaacaca acctagatct
    13741 ccacttatga tgtagattga gcaaatctcc actttcatag aaacgaatag gaatggcatt
    13801 ggggaagaga taggttgcat attcttcttt tttacattca tttagaaatc gatttactcg
    13861 ttcaaaaata ccattcattc ttttttcata ttcttctttt agttctggat atttttctat
    13921 gacagccggt gttatataat ctggctttcc tgcatactga gacagaagaa caggtctagc
    13981 tcctggaacc attctatgtc tttggtcttg gctatctgcg gtatgcgaaa tccttttctt
    14041 aaatgtataa tgaacctgag aaagagatct tgagattgga ctcatcgttg tttcatttag
    14101 ggtagaagtt aagtggtgat tatgggctgg cgacaagaca agtcgaatcg cctcttcatc
    14161 acttaatttt gacttaggaa tagagagaac ggaacgaaca gcagaagcaa gaattgctgg
    14221 cgaattctca ttgtagccta ctaatttaga ataacgtcct tccatatccg aatcaaattc
    14281 ttcgatgaat tctttcgtat agctaggatt gtaagcggat ttagacgaat agaatttttc
    14341 aaaaaattga tactctgaag tttcctctaa tggaatagga tcatccattt cctctacata
    14401 gagtgggtct acgcttttca ccgcttcaat catttgctct actactattc tttgttcttc
    14461 tggaacgtca tacgtattca taagcctatg ataacgatga agagttaatc cattgattgt
    14521 atgaaataaa taggtataag tagctagcgg taggagatac cgagcaattt ctaaactttt
    14581 tttcttaata ggatcttgcc atttttcggg gtaatttgca cgggcgcgat aaacagaaaa
    14641 atactctttc ccaatataag gatttaatct gtctataaaa tcaaaataag aatcggaact
    14701 ataattcact gcatctagat acaattccaa ttccttgcca tttagatttt gaggaatgta
    14761 ataattttct ttttttactt ctacatatct ttggcttact tgctcagaat tataaaaagg
    14821 gtgcgaatgc aaaaaagacc aaacaaactg acgggagact ttatcgattg taaaaataaa
    14881 ttgagggtgt tgtcttgtag ttaaatgacc tgcttttttt gtagatttag caacacggtc
    14941 tcgaatctct atcgattttt ctgatttgac catatcttct ggaagaagta tccctttgga
    15001 tgagtagcag gttctagcag ttgcaatggc taaattaaat ggttgtttac tgtagtcgat
    15061 taaattgaca attggcttcg gtgtattcat tttcattctc acatacatac ggaaattatt
    15121 atgtcgtaaa gacaagaatt attctaattt gctaaaaatg atttaaaatt ctatttaatt
    15181 ttaaacagga attgaaactt ttccttactc agaaaatagt tatttcctta ttctcagctt
    15241 aaaatattta cttgactcaa caaaactgct aattattatc tcaaaatctg tttaataaaa
    15301 taagtaagtt ggtaattccc gtgaaaaaaa ccgaaactga actcaaagag atcattcaaa
    15361 atgcagtaaa taaaaacaat attttaagca tgaagaccta tctactcagt gattatggtg
    15421 aaagcatact gaaaattatt tcctcaacta tcttagaaaa attcaatcga atggatttaa
    15481 tggatattgc ctactcttct gcaaaagaac tagtcatcaa cgcaacaaaa gcgaatttaa
    15541 aaagagtttt atacaaagaa atgggactcg atataaataa tccagacgat tatcaaaaag
    15601 gattaaacga attcaaagga agtctaacag aagataaaat tgctacctac aaagacttat
    15661 ttaaaaaaca tgatctacct gtcactgcca cattctatta cacaaaagac gttttaaata
    15721 ttaaaattaa aaataacttt ccccttctac caatagaaga agaaagaatt cgagataaat
    15781 ttcaaaaggc aaccagtttc tcaagtctta tcgatttctt tatggaatat ggagacaata
    15841 cggaaggcgc tgggatgggt ataacaatgg ttggaattct actcgaccaa tcaggcatca
    15901 ataaacatag ctttagtctt tattcaagtg ataagtataa agagactgtc gcaaaaatag
    15961 aaatccctct gcacgaagga tatctttcta aaagagaagt gtttgaaaaa gaactgagag
    16021 aaaaaggaat tacaccagaa gaatttagaa aaaatttcag ttataattac aaacactttg
    16081 ataaaatgaa atctgacgaa gtaaaaacaa attaaaacta tctaataaaa aaatagaaaa
    16141 atatttttag aaaagcaatt gcctccctga gactaattgg taacacttgg tcacgcagag
    16201 gcttttaatg catagaaaat tatttgtatt tacgatttta tttcaaacta tactttttac
    16261 acaaaaaata aatgcacaac aggtaactcc ctctaaaaac tcaaaaaaaa atcaaactga
    16321 tttagaaatc caaagacaaa gtttagagat tgagagagaa cgtcttacga aagaacaaaa
    16381 tgaattttct aaattaaaag aaagttttct gaaagaaaaa gaatctctag aaaaagaaaa
    16441 agaagaactc caaaaacaaa aagaagaatt agatcaacag aaaacaaaca aagcaaaaga
    16501 ttcaactgaa gaagactcca aagaagaaga tttttttaaa ttagaggagt ctatttctat
    16561 tgtctctaaa cgagttggag agtcagaaaa aacatcaaag acatccgcta tcgtatctgt
    16621 atacaatcgg caaacaatat ccgacatggg agcaagaaac ttagcagata ttttaaaaca
    16681 agttcctgga attgaaatat tatacgatca atttggatcc tataaagtag cattccgtgg
    16741 agtattgtct agatctggag tgttaatgct tctagatggc catagaatca ataacttcta
    16801 tgatggctct actttcttag atattcgtgc agatgcaatt gaaaaagtag aaattattcg
    16861 tggtcctgga tcctctattc atggaactaa cgcttttgta ggagtaatca acgtaattac
    16921 aagaagttca tccggtagaa atggaaacgg atacgttgcc tctcgactag gacaatacaa
    16981 tacaattgaa cctactgcat tctataatat taaaatggga gaaaattgga accttggaac
    17041 gtatgcagga tactatgaat caaaacgtcc ggaagttcat ttaccaaatg atcaatcttg
    17101 tggacggact ctctatagac aaggaatttg cacaagcgtc attccccttc cacttgccga
    17161 ctacatcaaa acaaatgata gaaaaaagca aacaaaccta tttctaaacc taaatagagg
    17221 ggatgatttc tatataaaag gaaaaatgat tcgagaagat agaggaccga atgtaggaga
    17281 gcttggctac ttaactccca attcagaact aactttcaat ttgatgactc tagatattgg
    17341 aactcaaaaa attgcaatta cagaaaaact atctttgagt gctcgtcttt atggggatac
    17401 attcttaaaa aaggatcaaa tacaagtaga aagaccagac atgataaatc atataggaat
    17461 gagtcctatc aagaagacag gatacaaata tgacacaaaa ggaacggagg taattcttca
    17521 atacaatcct ttgagtcaat tgtctattat gctcggagca caattagaaa aactaactta
    17581 taaagatgcg tatgtccaaa aaaactttgc gggaaataat acatcaaatc tctacccttt
    17641 tttctatgac tttgataatt tagatagaga tagaaatgat acaatcctag cttacttcac
    17701 aacagggaaa gcatttgtat ccgatcctga aagccttcag aaaggaaaaa caaaatctag
    17761 aaatatacaa gctgaattcc tacaagtatt atgggatcca tttaaatggc tatcaattac
    17821 aatgggcgtt agacgagatg tatactctga ttttggtagc acgataaatc cgaagagtgg
    17881 tttcgtcctt actccttttg aaaagacgag atatggtagc ttatctttta aatttttact
    17941 tggttcagcc tttcgggctc caacatttaa agaaatgtat gatagaagcc aaacctatca
    18001 atttggtgga gttttaggaa ataatacaga gcgttttcaa aattatggac aagccggtta
    18061 cggtccacta aagcctgaaa caattagaac aggcgaagtg ggaatggagt atcaaacacc
    18121 atacaagccg ttaaacattc aagcaaattg gtattataca cgaatttata acaatataga
    18181 tggagttaac gtctctagca caccaccagg aaaattagac atttaccata acttgagagg
    18241 cttgacaatt ctaggaacag aagtagaagc aaaacttagc ttttcaaaaa gaaattatat
    18301 atttgcaaat gtttcttggt ttcaagcggt agatcatgga ggactcaatg gaattgtaac
    18361 tagtgacaca aaaacatttt taacaagcat tccacaagcg agagcaaatc taggagtcaa
    18421 ttggaatcta tttaaatatt ttattttaaa taacacaatc tgggcttcct ctgaaaggca
    18481 atccaatgct cgttttgctt ttgaacgact ttctaccaaa ggctttcaat acagtcaata
    18541 ttacatatgg aacgttagtt taggaacaac agaagaactg aatgaaaatc tagaatttcg
    18601 attttcaatt ttcaatttaa ataattataa attgtatgat cactcaaata cggcaacgga
    18661 agattataac aatcgagcca ttccagctgc ctatatctac caacgttata ttgaatttaa
    18721 agtgacttat tttattaaat agagggaaaa aatgaaatca tacattctac tgtttttact
    18781 tttatctagt ttattttgca cacaaaaaca aaatcctttt gatgatagat ccgcgcttgt
    18841 atctgtagta aaccctttgt catcaggaac ccctaagagt tttggtcttg atctaaaaat
    18901 tgatagtcgg ctcaatcggg gatgtgaaac aagaattgga aatttgatgg cagatgtttt
    18961 tgcagacaaa ggaaattcaa ttttgggatt ttataatggc ggagctataa gagaagactt
    19021 tggcttacaa agtatttatc ccaattcaat tattcccaaa ggaactattc ccaactctga
    19081 ccttttaaaa aaactatttc ctctcactgg cggaaatttg gttcgtatca atctaaatac
    19141 ctatcgtctt aaacaagctc tagaagtttc tgttggcaga ttaaatgcaa gagcagaaag
    19201 aggaacagat gatattgatt cggatggtcc aacgcatgga aattgttggc tcaatccaaa
    19261 cttaagtgga gcaggaaggt tcttacatgt gagcagtaaa atgcaaatcg aagtaaatcc
    19321 agttgcaacg gcagttgtca taggtggatc aggaacgaca aacacacttg ttataacttc
    19381 agaaggaaga agaatcataa aaatcataat tgatggaata ttattataca acaatagcac
    19441 aggggatata acatctggct ggagcgctgg tcaatcaagt tgcacaataa aagggataac
    19501 ctactccaat agtgccgctt gtaatttttt ttcggcaacg attccaaagt ttcaattcga
    19561 cgggggagat tcaaacgcaa ccctaaatcc aaatatgtta gaagtaaata atgatggaag
    19621 tgttgtagtt tcacaaacag gcataggagc caatttagat gatggaacca tcttaaatga
    19681 ttatttgcaa aaatttacaa cgggaccagt ttatcctcgt atttcgggtc gaatcttaat
    19741 gccttagata aatttaaatc ttagaattag taaattcaat tctaagaaga gaataatcgt
    19801 ccatgagaga tgctttgttt tgaataacgc tctccacttt ataaatatct cccttagcct
    19861 cttctagaca agcgagaaaa aattgatcac tcgaattgat ttctcttttt ccggaatcat
    19921 ttccttttaa taaaacatca tcttttccat ctgatccaaa aaatagaaca tcctggtgat
    19981 ttaaagagat atgagatatt tttaaattat tgtttcgaat cttttcatat acgaagcttt
    20041 ctccgatttt ttgattcaca tcgttctcac cgagaaaact tgcttttcca tctctaaata
    20101 aaacgatagg cggatgacct gcatttaacg catgcattct tccagtctca tcatctagta
    20161 atgccagaat acaagaaata ctcattgttc cttgcataga atagaatact tttactaatt
    20221 caatataaca atcttctagc cactcttctg gtcttgtaat ttgaccggat atttctgtgt
    20281 attgagttct atttaaaata gatcgaatca cagacccaaa aatcagagca ccccctgccc
    20341 cctgcataga ctttcccatt gcatccgcat tacacaaaaa acaataattc ttatctccca
    20401 aacgaagagt atctgctaaa ataaaatctc ctccaatttg agaggcagaa tttctaaatt
    20461 caaatttcat tttctgattc aatgtgtaat caattttaac atgaggattt tcaataaaac
    20521 ttccaatgag tggatgaaga agatttgccg ttaaataata atctcccttt ttttgttcat
    20581 gtaaaatatt tacttcgtgt agaacatttt gtaattctct tgttctttct tccactttct
    20641 tctctaagtt gagattgagt tctcggagag aggagctgta tgtttttata ctcatcctca
    20701 tttcatcaaa tgcatctgcc agtaatccaa tttcatcatt ggagagaact tctatcttat
    20761 aatcgaagtt tttattctgc atttcttctg tcgcattttt tagatttaaa atgggtgtgg
    20821 aaaaatggag agagaagcgg atactaataa atatccccat taaactaaaa gcaatgatga
    20881 gtagaacaga aataaaaaca ctcttgtata aagataaatt gagataacga gtcgtaagtc
    20941 ctattcttaa tgtggcaagt tctctatcgc ctgcttttac atgatacaat acatcaatac
    21001 actcaagacc attgtgctcg atttgacgaa tctccatatt ctgaccggtc tcttcttctg
    21061 cttttatttc agatatgact ttatcttttg ttctttctcc atcggatcct aaaagagtct
    21121 tatctctttc aacgattgaa tttccttctt ggctttctag aatcacatat acaatttcac
    21181 gatcgttttc tactagttca gcagcacctc tctctacctc tgaaaaattt aaactggcaa
    21241 ttaaattctc agacacaagc cccatactac gtgtgatcat aattccccgc ttcaccaaag
    21301 acctacgcaa ttcagaaaga agcatacctg taaagaaaat actagagatt gttaagagta
    21361 atataacaac aaatgagatt gttagagtta atttactcct tatacttttt gaatctctga
    21421 ataaggcaga tattagggga atttttttca tacagatata atttgttttt ttagcatgga
    21481 catttgagga tttactgaca aggatttttt ccttgcaatc tatttttact ttctatttta
    21541 ttgaaattat tccaatgtaa gaaaaagcaa aactttttgc taacactctt gcatttaaag
    21601 actaattata cgtgaaaaga ctatttgaaa taattctact cttctgtgtc caattctctg
    21661 tttttgcaga agaatttacg atagtctatt ttgatccaga tggaaacttg cgaaacatca
    21721 caagccttca atcagaagga aacaaatact tccagtccat ttcccctgat attaatttca
    21781 tagtaattgc aagtgaacaa attctatcta cagaactaga aaaattaaaa ccacagatta
    21841 tgattgtgca atctttgtat tatacaaggc aaaaaaacag tctaggattt aaaccctttt
    21901 atatattctt agcgaaagac aatcatttgt attttaaaaa aattattacg atgaatccag
    21961 attttaaaac attagaagat ctagataaaa aaattgttgc atctgctctg cccaaggata
    22021 caaccaagga acttacaggt ggagagagaa actggaaaat tctcactgtt ccaaaggata
    22081 tggatgcact tatggcgatc aaattcaggc aggcagacgc tgcgcttgtt gcagaaagta
    22141 gtctagacaa ctttaaaaaa ctagatccta cagatgctca aaattttaat tctatattta
    22201 ccaccaaaag tatattccaa ccgcttgttg ttacgactaa gtatgcaaca aacaaagatt
    22261 tgattcctaa gataatggat tctatgaaaa aaatcaaagc agacactaac ggtcagaatt
    22321 ttttacgtat gttgaacttt gatgatattt ccgacaataa aaaagttttg gggtctgttc
    22381 catgagattc atatcctatc taattgctct tttactttta atcaacagtt gtcaaagccc
    22441 gaaccgtatc ttagaaggga tgaaatttca ggggaataaa aagaagattt ttatcctagt
    22501 ctcagaacaa atttctagct ttgaagttgt gaaaaactcc tttatggaag aaggaaaaaa
    22561 ggaataccaa tttctttctt taaatctaga tggaaactat gatgacttag aacaattacc
    22621 agctcaaatc agaaaattaa aaccagactt aattttatgt attggatcca aatccttgga
    22681 aagtttagcg ggaaaaatca cagaaattcc aatcctattc gcaatggtat tggactacca
    22741 aagatttcac cctgaaaaat atggaaatat tgctggagta tcactgagca ttccacccga
    22801 aaccgttttc tataatttta aaaactattt tggcgaaatt aaccaatatg gtgttttatc
    22861 tagcagagat catatcagtt ctctcagaga gaatcgagaa agactcaaaa atgtgaacat
    22921 tcaattgaca attggagaga ccaattctcc cgatgacgtg caaaataagt atgatgcgat
    22981 taaaaagagt atccaagcac tttggatggt tcctgatccg aaagtaatct cagaagaaag
    23041 tttcttgttt ctagttgata atactataaa cgataaaaaa agttttatcg tttactctga
    23101 acaatttgta aaagcaggag cactctttag cacttctccc aactattcta ccattggctc
    23161 ccaacttgca attaaatccc aacaaatctt agaagataga caaaagccag tgcaagtagg
    23221 aatttcccct atcatcggaa cctttttaac cctcaataaa acaacaatga aaaagctcgg
    23281 cttgaacgta cgaatggatg gaatcgacca agtcttcgaa taatcaaaac tacgaaagat
    23341 tatctagcta gttaagaata atctatttgc gattgcctcc tatttgactc aaaagactat
    23401 ggcagttagg agcaaggaat ggagaaagag aaactacaag caattgcgaa tctagcaaaa
    23461 ttacgcatag aggattccca attagactca atggtaaatg attttaataa aatcatggct
    23521 tatgtggata gtgttaaaga attaaacacg gaaagtgttt cagaagaaga aatctatgaa
    23581 tggtcagaaa ataaagtgcg accagacatt ctaggcaaat cactcacccg cgatgaaatc
    23641 gcaaaaatct cccccaaatt tgaaaatggt tacattgtag ttccaagggt aattgaaaca
    23701 tgatattcaa aaaatacgca gaaataaaaa aagacttaca agaaaataaa atcacatccg
    23761 ttgatctttg caaagcctac ctaaaaagaa ttgaagaaaa cgattccaaa ataaaagcat
    23821 ttttatattt aaacaaagat agaattttac aagaggcggc tagctccgat gaaagaagaa
    23881 aagcaggaaa agctctttct gatatggatg gaattccgat tggaattaaa gacaatattt
    23941 gcatcaaagg agaaagagtt tcttgtggtt ccagaatatt agaaaatttt gtttcgcctt
    24001 atgatgccac tgccattcag aaactaagag acaaaggttt tattttcttt ccaggtctga
    24061 atatggatga atttgcaatg ggctcctcta ctgaaaattc agcatatcag gttacaacaa
    24121 acccctttga tacaactcga attccaggtg gttcaagtgg tggctctgct gccgctgttg
    24181 ccgcttctat gcttcctgta gcacttggat ctgacacagg aggatctata cgtcaaccgg
    24241 catctctttg tggcgttttc ggattaaaac caacctatgg aagaatatct agatatggac
    24301 tcgttgccta tgcatctagt ctagatcaaa ttggtcccct ttcaaatgat ccgcaaggaa
    24361 ttatagatgt attatccgtt ctatctggat ctgatttaaa tgatagcaca acgtcacccg
    24421 aaaagccctt cactgtttcc gatctcacaa aaatcgattt aaagaataca aaaattggaa
    24481 tcatggcaaa tgaaggggct gaatgggaga aggatgtaaa agatagattt catcaaatta
    24541 tagatatttt gaaatcgaaa ggtgcaaaaa ttattgagct tgactttagc attcattcct
    24601 acgcgattcc aatttattat atcatcgcaa cagctgaatg ttcttccaat ctgtctcgtt
    24661 ttgatggaat ccgttatggg tatagagtgg aggatcccaa aaaattagaa gatgtctata
    24721 ttgaatctag aagcaaagga tttggaagcg aagtaaaaag acgtatttta ctaggaactt
    24781 tctctctctc atctggatac tatgatgcgt attacggaca agcgcaaaaa gcgagagtgc
    24841 ttatcaaaaa aagctacgct gaatatttta aacaagttga ttttatctta caaccaacct
    24901 cacctaccac agcatttaag attggagaaa aaacaaaaga tccagtgcag atgtataaag
    24961 cagatattct tacaacaagt gtaaatttag caggacttcc tgcgatcaat attccagcag
    25021 gtgtggataa caaaggtttg ccgatcggat tacaaatcac tgccaataaa ttctttgaag
    25081 gtaaactact ttcttttgcc tctgaggttt cgagcttaga cgaatgcaag gtaaaactac
    25141 cggagaatat tcaatgacag aactcactaa aagagtgatc ccatgcctag atgtaaaagg
    25201 aggcagggta gtaaaaggga ttcaatttgt agacttaaga gatgcagggg atccagtaga
    25261 atgtgcgatt tcttacgaag aacacaaagc agatgaactc tgttttttag atattactgc
    25321 ttcgagtgat agaagggaaa ttctactcca tctggtagaa tcagttgcta gcaaaatttt
    25381 tattccattt accgtaggtg gtggaatcaa ttctgtggct atggtaagag aagttttagt
    25441 aagaggagcc gataaggtat cgatcaatac agccgctttc gaaaactcta aacttttaaa
    25501 agatgcaagt gaaatctttg gttctcaatg tattgtttgt gcgatagacg ccaaatatca
    25561 cagcgaaaga aaaagacacg aagtttttct acatggtgga agaacagaga caggaagaga
    25621 agttttagat tgggcaagcg aagccgagga aatgggagcg ggagaaattt tactcacttc
    25681 tatggataga gacggaacca aagatggatt tgacattcct cttctaaagc aaatcacaac
    25741 gaatttaaga attccagtca ttgcaagtgg tggagcagga aatgcggaac atttagtaga
    25801 agcaattctc agaggtggag cagatgctgt ccttgcagcc tctatctttc actttaaaga
    25861 acaatcgatc attgaagtaa aacaaagtat gaaagaaatg ggtattcctg ttcgtttatg
    25921 agtttattca cttggattga tatattcatt atttttattt ttttattttt ttctcttttt
    25981 cttccttatt tattttccaa aaaagattct actctcaaag gttattttca agcgagcggt
    26041 agaatgcctt ggttcatttc tgggactgca atggttgcaa caacgtttgc cgcagataca
    26101 ccgttagccg taacagagct agttgcaaaa tttggaattt ctggaaattg gctttggtgg
    26161 tatggttcaa ttagttcgat tgcaacggtt tatttctttg ctcctctttg gaaaaaatcg
    26221 ggagtgatga ctgatattga actcgtaaat cttagataca gtggaatcgg cgctgagatt
    26281 ctaagaatct ttaaatccat ctatctaggt tttttcatga atattttaat ccttgcttgg
    26341 gtaaatctag caatggtaaa agttgtagaa gttttacttc cagacgtttc tgcgaaatta
    26401 attgtgagta gcttatttct atttgccttt ctatatacaa tttatatggg attaaatgga
    26461 atttccattg ctgatacatt tcaatttttc tttgcgatga ttggatgtgt tgttcttgct
    26521 tattttgttt tatcgatacc agaagtgggt ggaattgatg gtttacaaca aaagcttccc
    26581 ccatcgtatt ttaactttat tcctgatttt cagaatccag cttccaaaga tttttcagta
    26641 gaatcatttc ttttatttat tggtgtaata tggtggtcta gttggtatcc aggagcagag
    26701 ccaggtggtg gtggctatat agcacaacgc atcttagcgg ctaaagatgt agagtcttcc
    26761 ctactcgctt ccctttggtt cacaattgca cattacttta taagaccctg gccttggatc
    26821 ttagtagcac ttgcaagcgt tgttttattt cctgaccttt cgctcgaaga taaagggaaa
    26881 ggctatgttt tgattatgac caaagaaaac ctgccagttg gacttttggg tcttgtaatg
    26941 gcagttttta tggcagcgta tctttctaca atcgcaacac atctaaattg gggttcatct
    27001 tatctaatta atgatcttta caaaccttat ctagcaaaag aaaaaagcga caaacatcat
    27061 ttagccgctg gtaagggatt ccagctttta atgatggcat tttccctttt gatttctttt
    27121 tactggatta aaacaatctc aggggtttgg caatttttac tcgaatgtgg agcgggagtg
    27181 ggaatcgtgc ttatcttacg ttggtacatt ccaagattaa atgcttggtc agagttaaca
    27241 ggttttattg caccttttgt tttttactcc atctctaaat tcatcttgca ttgggagtct
    27301 cccacttctg ttctatttac agtcttctgt gttttgatta caattctatt tatcacattt
    27361 ctaactccta aagtagattt ggaaaattta aaatcctttt atcaaaaggt aaatcctcct
    27421 ggtctatttt gggcaaaatg ggcaaaagag aataatttag aatggaaacc ttcagaatta
    27481 aatctgctcc atagtcttct actatccgca accggaattc ttttgatatt cagcggtctt
    27541 tttagtttag gaaatattat tttaaaaaga tatgattcat tggttctaag tatttccctt
    27601 tttatctttg caattgttct cacttatcat ctatttccga gacatctcga tttagagaac
    27661 aagaaagaat aattttcact aaattgattt gacacttagg cctgaaataa aatcataaca
    27721 atatcgcgct tatggcgtcg tggccaagtg gctaaggcat ggctctgcaa aagcttgatc
    27781 cccggttcga atccgggcga cgcctcggtc gctaacaaaa tgctgggtga gcgcctagcg
    27841 aacagggtgg ataaatcctg tgagaggcga ctctcgaaaa tgcgaatgtt ttatttatgc
    27901 ctaggtggtg gaactggtag acacctagga tagcttggct gctgggtgag cgcctagcga
    27961 acagggtgga taaatcctgt gagaggcaac tctcgaaaat gcgaatgttt tatttatgcc
    28021 taggtggtgg aactggtaga cac
//
LOCUS       DAOQOS010000072        27931 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_775_length_27931_cov_5.721481, whole genome shotgun sequence.
ACCESSION   DAOQOS010000072 DAOQOS010000000
VERSION     DAOQOS010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 27931)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27931)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27931
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_775_length_27931_cov_5.721481"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..310)
                     /gene="metX"
                     /locus_tag="PLF66_15590"
     CDS             complement(<1..310)
                     /gene="metX"
                     /locus_tag="PLF66_15590"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016752331.1"
                     /note="Catalyzes the conversion of acetyl-CoA and
                     L-homoserine to CoA and O-acetyl-L-homoserine; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HMZ65518.1"
                     /translation="MNLKDSVGIVETKIAKLPDLTLESGQVISPLEIAYETYGVLNPN
                     KNNVILVCHALSGTAHAAGYHAGAEKPGWWDGYIGPGKAFDTNKYFIISTNVIGGCNG
                     S"
     gene            complement(314..1621)
                     /locus_tag="PLF66_15595"
     CDS             complement(314..1621)
                     /locus_tag="PLF66_15595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010574953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HMZ65519.1"
                     /translation="MARQFKPETIALHAGYTKGDPATTSRAIPLYQTTSYVFKDTDHA
                     ARLFGLQEFGNIYTRLMNPTTDVLEKRVAELEGGIAALATASGQSAETLALLNIVEAG
                     QEIVASSSLYGGTYNLLHYTFPKLGIKVHFADPSDPNNFKKYFNDKTRAVYAETLGNP
                     KLDTLDLEAIAKIAHDHGVPFIVDNTLPSPYLVNPIEHGADVVVHSLTKFLGGHGTSI
                     GGIIIDAGKFNYGNGKFKNFTEPDPSYHGLKFWDVFGKFEPFGGANIAYIIKARVQGL
                     RDIGPAISPFNAWQILQGIETLPIRMERHSSNALKVAEFLSKHPKVSWVNYPGLPSDK
                     NYSLAKKYHKRNLFGAIIGFGVKGGVPEAKKFIDGLEIFSLLANVGDAKSLAIHPAST
                     THQQLTEEEQRSAGVTPDFIRLSVGLEHIDDLITDLDEALKKV"
     gene            2235..2573
                     /locus_tag="PLF66_15600"
                     /pseudo
     CDS             2235..2573
                     /locus_tag="PLF66_15600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010574761.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspG"
     gene            complement(2621..4588)
                     /locus_tag="PLF66_15605"
     CDS             complement(2621..4588)
                     /locus_tag="PLF66_15605"
                     /inference="COORDINATES: protein motif:HMM:NF025890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HMZ65520.1"
                     /translation="MELSKKKKWAIRIILFWIIFFGVVYLNYSNIINQIIPYVHRSEK
                     ISYPTIKVTDAEFEDIAKQLQDEFSNYKSVDKSIQNKAVYDAVAKNLSDKVSTFQRNT
                     GLVHIDRFRKAGIREYKGPETCLSCHETMKVKNDHGGYDTVNTRANIESSIHFSLNKF
                     EGFNTYGFNGERVKGIPLGKIDRACGIPGSFTWTGWATIVNTKHGPRSDGCGQCHAGG
                     QYGPISGTMFPGYKPKSFEFESTDCLMCHSAAYDMNQKYVVQDENGKYRWNQDRSLKA
                     AMAAIKPNSDNCLRCHEHNHGGDMYPGNKAAMNLGYKNPRLLHPGAKRGTPTTGIDVH
                     YRAGINCIDCHETHGHLIARGKSGTDLVSNDLPNVEVTCEKCHSSSPHIQNKNQRAFL
                     NAHTDKLACETCHISHLTDENVVLRDWTEPVYSEEEGVWIYKDILRSGKPGEAIIYKW
                     FNGSGTFMAGALGDNPNGLKTFKAFKTNPDEPYKNFDYDSYYEKTFRPIASQGKSKLT
                     PFKRFNAKMFEDMGNQGPFGGMLLPFDYNVYYETGKPKDAVMKAVDDPLIKLMYGNMF
                     KYYMMDKFMYFMGIEKGWTIPFSGKVEPKWMRQDATLMINHSITKEAMKCESCHTSKE
                     KGVMAFEELGYPESRIKDLRNLEELKLIEKK"
     gene            complement(4593..5300)
                     /locus_tag="PLF66_15610"
     CDS             complement(4593..5300)
                     /locus_tag="PLF66_15610"
                     /inference="COORDINATES: protein motif:HMM:NF013460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HMZ65521.1"
                     /translation="MKKENSEKKFLRLSLEQRIQHGFMILSFTLLSITGIPMRFPDVS
                     WVKYIYFFFGGLQGARIVHRVSAVIMILLGLYHLYYIFRLLVINRFNVSKAWPMIPNL
                     KDVKDWWETSLYYFGLKEKLPLYDRFNFREKFDYFAVFWGVPVMMFSGLILWFPVFFG
                     NMLPNIAIPMAYIAHSDEAILAISAIVVWHFYNVHYNPDKFPGSTVWIDGKLTEEEMK
                     VEHPLEYERITGNKIKE"
     gene            complement(5313..6332)
                     /locus_tag="PLF66_15615"
     CDS             complement(5313..6332)
                     /locus_tag="PLF66_15615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65522.1"
                     /translation="MKSFLTKQIPLEKKKFIYVSFIFIFLFFTLGIHSDDLQQNCNKC
                     HAMNNFSYLNEDLKSLKNLTVNTEEFKNSAHGKLNCIDCHKETKSYPHNTDLPKVGCN
                     SDCHSIDKEGKTYTHSKQENEFNDSIHTKGKIKSNPDSPTCINCHGYTNVHNIKKVTK
                     TITQGEKISQCIVCHDNKEMMIRNKVEADAVESYRQSFHYKAVKFGGISSATCQDCHA
                     VHKILPKDNQEASIHPNKVSDTCGQSGCHEGVKLNFAMSGANHLKLRVEKEPILYWEE
                     KMFQILTGGTMAMLVVGIILDIQKKFGWFSVFEKLGAFIKEGFLLFITVLKKVLKLLK
                     ALLYD"
     gene            6891..8513
                     /locus_tag="PLF66_15620"
     CDS             6891..8513
                     /locus_tag="PLF66_15620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012476514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HMZ65523.1"
                     /translation="MSEKTYRKVYKWGDSAKEETLNHHTAEFLEKNFFIQVKKEPYLP
                     GNTEVSIKKKSSLSKAVKDKLISIVGSENFYDSDFERARHSIGRFYTEILKARLGQIE
                     NAPDAVVVPRNQEEVAKIVEVCNTNKIPIIPFGGNSSVTKALQAPKGGISLDLKNLNK
                     ILDFNETNCTVTAESGVFGPVLEKFLNDRGYTCGHFPQSFEFSTVGGWVAAKGAGQAS
                     TGYGKMEDILLGLTAITPNGKLESKIYPAASIGPDLFKFFLGSEGIFGVITTVTLKIR
                     KYDPKNSSMTSFIFPDFESGVNAMREIMQGGFGKPHFFRLQDPEETDIAFRMSGKEGS
                     ISDKFLRLMKFTPMKRSLMYVIVDGDPDYTKFLIRKVRKIAKKNGAFEVGKSPVHKWL
                     EQRYSSAYLRDTLMDQAVMIDTLETAVTWDKLLILWKAARDYIKSRPQTLSLVHISHA
                     YENGANLYFIFMSPMQKEKELEEFNKFHSGLIDIIHKNHGSLSHHHGIGRLTSHLMKE
                     EVGGFGLEVLQGLKKCFDAKGIMNPGGTLGLK"
     gene            8500..9150
                     /locus_tag="PLF66_15625"
     CDS             8500..9150
                     /locus_tag="PLF66_15625"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HMZ65524.1"
                     /translation="MVLNDLNKPQSRKEREFIKRGEEILEVAKTLFKKQNPALVSMDM
                     IAQAVGIGRGTLYLHYKGKDELMIRIVLNRHKELLSDLEKMDLSLPGEELARKAIRVY
                     LEHNLKQPEEYLMQKKCEENIIKENAGEEILKEVEEERLKRIAIIEKVFIKLKSEGII
                     RDIPPYLLAGAIWGMLKGTLDIIVGGHFKQEIEDEEMFFQLVENLLFYGLFKTKEK"
     gene            9153..10796
                     /locus_tag="PLF66_15630"
     CDS             9153..10796
                     /locus_tag="PLF66_15630"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010419173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HMZ65525.1"
                     /translation="MNTNHLRKNRLQLKDLSTEMFDVVVIGGGITGACIFWDATLRGM
                     KCLLVEKNDYASGTSQATSKLIHGGLRYLKNLEIGLVRESLRERRNLARISPHALYTM
                     AFQIPVYNTKDVLMTKFGMMLYNQLSYDRNKLIHTDNLIPEYRFHNREETIYEVPEIP
                     RDHLKGSYLYYDYANLNPERYTTEFIFSARSQGGICRNYTEVMHLEKNGNGIYKIQLK
                     DNITGEVGLIETKTVVNSTGPWADKLESMMFSGKETKLVRSKGIHVVTRKICKDTTVV
                     LKKRNGTHLFVIPWRNKTIIGTTDTVYSDSPDVFKVTQKDILELLEEVNYAYGSIQLK
                     LEDVDFYYGGLRPLVEQEGKNTYNASRKLEIMDHKEEGFAGFYSALGGKYTTSRLLAE
                     KLVDKIAEYLPKNFSECKTETTPILGGNFSDFKTLEQELQKEFPKESGEKIEILVHRY
                     GSEARTILKTEIEEKEIFHLQNGEHIYSEEIAHIALKEDIVYASDFYFRRSGVGVPGK
                     PDPMNNDKILNTLGKYLNWDKSKLAAEKNCIENRYKIIA"
     gene            11040..12170
                     /locus_tag="PLF66_15635"
     CDS             11040..12170
                     /locus_tag="PLF66_15635"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HMZ65526.1"
                     /translation="MKKKILIVDDSETSSMFLKMALQNYDVNSVTSGREMWKLLESEI
                     PDLILMDIVMPEEDGYSLSQKLLLNDQYKDIPIIFQTTKNSSDDLEKAFDTGAVDYIS
                     KPIDEKELRARIKSALKIKELEKELNSALTVIKQDLAVAAKIQNKIIPDDVQVRNGLV
                     FYTKYLPMVEVGGDFYYITEIGSGITRIFLADATGHGVQSALITMLIKSEYENLAKRS
                     ESPSELLYHLNETFINKYQVLKTFFSAFVVDVNINLNTLTYASGGHPEQVLVSGKTNT
                     IELLKKTGRLVGVVPETKYKEITMEFVKGDRLYIFTDGLYEEFNEKEEIYGEEELFDI
                     LQNNNRNPIKQIIEFILSDLKDFMGTRQQDDDITVIGLEIAP"
     gene            complement(12139..13254)
                     /locus_tag="PLF66_15640"
     CDS             complement(12139..13254)
                     /locus_tag="PLF66_15640"
                     /inference="COORDINATES: protein motif:HMM:NF024827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HMZ65527.1"
                     /translation="MRIHLVSYIGFTLLAALFLTQCKQKNIHGFNKGLTAIEVESDIE
                     SSKQCQKCHKEIYREWETSRHKVAFTNDLYRESHRREPLVWCVNCHAPLLKSGGNLEN
                     LGDRVFIEEGVSCIVCHKRGDKILTAHVPQNPKEHSYLEVKSMKKSEFCENCHQFNFP
                     TGTGKVPHREFKFSRQPMQNTFNEWQMSYFYGKENCQDCHMQTISDYRTHTFPGGHSK
                     DYLERTFSLQLYKIEETEIKIILEAKRLGHAFPTGDLFRTLVIKLYDKDNLEIRKILL
                     RYEYEEMEANKNSQTDSAKKLKSRSIITPPVLKENAYFIKNIKMEKNETDRIRFYELS
                     MQYVNESNELFQDSNETENALPFKKETIKVLSQDQLQ"
     gene            13497..16628
                     /locus_tag="PLF66_15645"
     CDS             13497..16628
                     /locus_tag="PLF66_15645"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HMZ65528.1"
                     /translation="MELFQNFYFNYYTIGVITGLFMTGLIALFLSLLPKKSTPTKYLV
                     FIYLTGALVSIGYALVQGIYLPTKWIRIYLLIAITLNHWLSIQLFFQFPENNYPKTAK
                     GFLYSQGIIFFLLDSFLIYKAMNSGVVYDFSGHYYELDSPIALKIYGLIVLVNIFVIT
                     GVSIWRVIKTKTDEKLAIAFIALGFGLAFFVPGLINVLNKSGIVSRTVYITIYCMSTV
                     VGLFMVIVAYINKTKDNTTFMFKIVGISFVVFMVVYDIFAFIVMKEVETTYDIIHNHS
                     TILAMEAKHRPDDLKYVVEYDPEKDTFTYIQNTDNIELPENFKYDLINSYVFDSFSKS
                     KEASLIEMKQILSNLKIKDSEYLSGYASLINEYLEKFEPETGVSKADVNSLILNKERF
                     LFIRSNKVDQLDEKDFRKNLESLLQKEKEDFLPFKQAIEKHLANSNKDGIELKKEILN
                     FLVPMQDTSLRRYRKSLDLKNHYVTFHYIDREKNKIYEIGYSYLEYRKFIHAVAVKLI
                     YILLFALVLMVIGIPIFLSGTLINPLNALLEGLGKVKKGNLDIKIPVKVQDEIGFLSN
                     SFNNMVQSIKDSKEKLEEYAEHLEEKVEERTKELQVSLNQVEKLKSQQDGDYFLTTLL
                     LKPLGVNKVHGSKIKVDFFVKQKKEFKFRAHEHDIGGDICITHQVKLKGRDYITFLNA
                     DAMGKSMQGAGGVLVLGAVFQSIIQRTVSYRAHSEIPPELWIKRAFKEMHKIFESFDG
                     SMLISLVFGLVDEKSGHVYFINAEHPWVILYRDGVADFIEHDMKFRKLGTSGLDNELA
                     ISTFQMMPGDFLIIGSDGKDDLILSKDVKSGTRVINEDEKLFLRRVEEANGDLNKIFE
                     LITNKFELMDDFSLLSISYPNEEIELNEDEANQIAVTLGNARKLVGEHKFKEAIEILE
                     VSYEQNPNRLEVARYLIKAYMKQRRYKEAARVCKEYLDKNELDSSLMFKASYCLKMNH
                     EFKEAIEMAERIKLREPLNIRNLIHLADMYTYNKNYDRSYKLLRKILMLEPENKIAEK
                     ILERVEQERKLVV"
     gene            complement(16625..17185)
                     /locus_tag="PLF66_15650"
     CDS             complement(16625..17185)
                     /locus_tag="PLF66_15650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65529.1"
                     /translation="MPLKHILIFLAIYLSIIPILSWENDFYEKPQYVTYKFPDEKLPR
                     HSFTRFPQAGVMISSFCIYKSGSGRKGRECGAWKPYEYIKTGKLVPLIREKGFFSKGD
                     SPKQIAFLVCSESGGTVETEANEYNPNGREIELEDFCSFKDGSMTSFNFILLYIARRG
                     QNIRTEIEKVKQERLKLLPSPPAKTN"
     gene            17357..20737
                     /locus_tag="PLF66_15655"
     CDS             17357..20737
                     /locus_tag="PLF66_15655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016751002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HMZ65530.1"
                     /translation="MRKVIEYFLSKSLLLNLLTVLIILVGSYKALTMNREAFPNINFD
                     IVTVTTIYPGASPAEVEKLVTKPIEDSIKSVDGIKEYRSASIENRSGIVITIDPNAPD
                     TQKVVDDIKSAVDRTEDLPEDSKKPIVQEVTSSRNPVIEINVGVKADASGKPVLTEKQ
                     FKEKAKQLEDLLLDVPEVGRVVRRGYREAEMHVDINPLALDRISLSTNQIIMALKGKN
                     VNFPGGTIADAGREAIVRTVGEFDTESEIEKVFIRSNDAGRSIILKDVAKVTEDFEDK
                     QYIEKVNGLYSIVLVVVKKERGDAIKLVDKVKKVVTDYGATQSDYITLSYVNDLSKYI
                     RRRLDVLVSNGLQGFVLVVISLFFFLGWRVALMVALGLPLAMALTFIILDYMGMTLNL
                     ISMLGLIIVVGMLVDDAIVISENIYRYLEEGVEPYEACIRGTIEVIAPVTATITTTIA
                     AFGPMLFVTGIFGKFIYSIPMVVIIALSSSLFEAFFILPSHIYDVNKHGIKKGEIKEE
                     GHWFVKVRERFYRPSLSWALKHKGLTVLILVTVFAFCIGLNAAFGKFKLFPGGIEVFI
                     VKISAETGLTLEETEKFARAVEREIALLPQYQEKKANGFVERIQYFFHSLGLVIQPKF
                     HTGEVENYVTRVGIIQKDVNDPFTKRGKNFAQVVVYLTPDAERKRSTEQIINYVRKRT
                     EWLMNEKALAVAKQKELARRNAEKKKNKPSDEENIPIEIPEEFKELKGKLVSLDLEAI
                     QGGPPVGKPVAIEIRGDDFTTLKKIAQDFKDVMGQVEGVIDIGDDFNDGKDEIRLTID
                     EALAAQAGVSVQQIALAVNTAYQGTVATTIKRADEEVDVRVRFPENFRTSIRSLDKIF
                     VNNNMGNLIPISRMTKYEKVPGLASINHLDGKRLLTATANLDETKTTSMKANQEIKKL
                     ADAAKIMDKYPGYRIRYGGENKDTEESLASLGRSFMIAFFIIFIILVSLFRSFVQPLI
                     VASAIPFSFIGVIIAFVTHGQYFSFLSFIGIVGLSGIVVNDSIVLVDFANQLRSQRPD
                     MSSYDILLETGCVRLRPVILTTVTTVLGILPTAYGIGGSDPFLMPMALAIGWGLAFAT
                     FLTLIIVPVFYKIVMDFQIWFSDKFFRKDIKTLAQEQA"
     gene            20772..21866
                     /gene="ychF"
                     /locus_tag="PLF66_15660"
     CDS             20772..21866
                     /gene="ychF"
                     /locus_tag="PLF66_15660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010574732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HMZ65531.1"
                     /translation="MSLNCGIVGLPNVGKSTIFNALTKAGAQMANYPFCTIEPNKGIV
                     EVPDKRLDRLAELYNPKKVIPTVMEFVDIAGLVKGASTGEGLGNKFLSHIREVDAICH
                     VVRAFEDANITHVHGKVDPVEDVSIVNYELILSDLESLEKQYPKLQKLGKTNKEAQES
                     ATVMERIINILKEGKSARLLDLNADEKKIAQKFNLITLKPVLYCANVLDSDIGNKSNP
                     LLEKIYAMAKEENAEVVVLCGKIEEEISGLAPEEQIEFLKDIGETESGLNRMIRSAYK
                     LLNLITFLTAGEQEVRAWTTLAGSPAPKAAAVIHSDFEKGFIRAEVMAFTDIDRLGSP
                     VKVKEEGKLRLEGKDYIVQDGDVIYFRINA"
     gene            21923..24004
                     /locus_tag="PLF66_15665"
     CDS             21923..24004
                     /locus_tag="PLF66_15665"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HMZ65532.1"
                     /translation="MSSEKLSIADLVAESGSRVLAFIARYTSYVFAVSIFSAGFSGAV
                     STVFTLYYFDAVNVSVSETFMFILKALIFTYWIILMIQHGLLGRFGIPGFGLHIKTVN
                     RYVKSNPSIEISKDLSEEEYKELFKSIVALPGFLAFNLFIWVFVLILIAMMLGIFLEG
                     FNGSVIFSLLTIDIICLYDTVCITLIASEIMTGSIREKCKEIMNSKGIQFRDRALSTV
                     LTKFILFLILLAIALYLSNVVTYYNHDHIEKVISFSILALVVSCLLAYMIFRIIYNAL
                     KQIESAAYDLMRGGSGLIFPRSLDKEFINVAIGLNKASKTINDYKISLEEKVQKRTSE
                     LNAANNELIAKGQIAEQELELARNIQQGIIPSDLKTWNGISFAAYYEPMGKVSGDYYD
                     VFYFSKKIFVLMADVSGHGVPAAMITMAAKQAFLQVIQEEKKPADIFKEVNQIILDQV
                     KTSDYLSAFLLRIDEKNRLTYGNAAHPQAIHYIHSREEYQLLDTEGMFIGAVEEASDF
                     YANSELKLQSGDRIYLYTDGVLEHKNTSGEQYGMDRFIETLSRNRNQSLSIQINEVIS
                     SLKKFMGNAPIRDDISIIAIELDPNWAKFIDLFNQGLKNLREKKLEEALAQFLNALKI
                     VPTFLAIQFHLAAIHFHLGDLDKSLEHILVYLKDNPLDKKAIQLIINIYSNQGNEKEM
                     NKWLTQFNNIH"
     gene            complement(24184..24774)
                     /locus_tag="PLF66_15670"
     CDS             complement(24184..24774)
                     /locus_tag="PLF66_15670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65533.1"
                     /translation="MKIRNNLFLILFHSKEEDRWESWKRFAEKNNYTYSLLSDPVIRP
                     ILRGTLYDKPICIEAAYTNILETDLSIRIQSPIHNPQELYFLVEDKKAYKSKKGIFDK
                     TISGFCKNRELEEKFFIKSNSETFPIKMLNRNNLTNDLMGQDNFAIEVANYELTLTTY
                     NILSIDKEFIGFLKLFYSFLSAFEETTIESFLYRKQ"
     gene            complement(24825..25838)
                     /locus_tag="PLF66_15675"
     CDS             complement(24825..25838)
                     /locus_tag="PLF66_15675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020768505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HMZ65534.1"
                     /translation="MDGNTEIINLDPPKFLNNSHVQTIFTTIFPPKNHLKTKYESDVL
                     ILKTKDESGDFLWLDHNPPLQKMDKKAVPYNGHYMILFHGMEGSSDSHYIVSLAEAAL
                     LQGYGVIRVNLRGCGRGEGLSKKGYNAGKTDDIGQIEQYVYTHFSKKIIMCGFSLSAN
                     MVLKFFGEKRVIRSKVFSAVSPPLDLKRACEHIDSPAGIFYRKIFLDSFKEKFKKGFL
                     FASPDIKEHALKAKTMFDFDDMVTAPLFGFKGALDYYKSSSSIHFIPKIKHKGIIIHA
                     KDDPLIPPDSFQSVLWKKNKNLTAILTETGGHVGFITRKTEEIPDGKWINFILLKFFK
                     KFL"
     gene            complement(25817..26149)
                     /locus_tag="PLF66_15680"
     CDS             complement(25817..26149)
                     /locus_tag="PLF66_15680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65535.1"
                     /translation="MSDKKDLESIFRLYLDATKRDDGEIIVLILNYLSHLFVDEEGEN
                     TEPILLEDFTHYEVDDFLNFYLEDNFENFLELQAKSKIMLRNFLKFLKERKYFNKEEV
                     EEWMEILK"
     gene            complement(26139..26777)
                     /locus_tag="PLF66_15685"
     CDS             complement(26139..26777)
                     /locus_tag="PLF66_15685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002974223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HMZ65536.1"
                     /translation="MNINPTNLLKSDDEKEGDEKSQNVLEELKNGNFIQKKFLDTRKI
                     FLWGPVMDDSAKEIVNKLMYLEMIDPGKEITFYINSPGGSVTAGMTIYDTMKMISSPV
                     ATVCMGMAASMGSLLLCAGTKGRRFIWPSGKVMIHQPSIGGQIVAPATDIKIHANEIK
                     KTKEQLNKILAEACGQPLEKIALDTDRDYYMTAKEAIEYGIVDALATSIHVG"
     gene            complement(26793..27236)
                     /locus_tag="PLF66_15690"
     CDS             complement(26793..27236)
                     /locus_tag="PLF66_15690"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HMZ65537.1"
                     /translation="MLSKNQFSISIGLLSIFCIIYSVLLGANGIIERRTLEKRLNILR
                     EEVERLETENMNLEAKRKILREDENALAREARRYYLLSEDSKVLKFKEAVTNESSDRL
                     FASGLPAANVKKKETKEDLPPLNMLKFFYIVGAGSILIGVFMKFK"
     gene            complement(27226..>27931)
                     /gene="eno"
                     /locus_tag="PLF66_15695"
     CDS             complement(27226..>27931)
                     /gene="eno"
                     /locus_tag="PLF66_15695"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020763879.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     2-phospho-D-glycerate in glycolysis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HMZ65538.1"
                     /translation="NTAVGDEGGFAPDLKSNAEGFEVILEAITKAGFKPKDDVLIGLD
                     AASSEFYDKKKKKYVLKAEKNPEKTSEELVNYYAELVAKYPIITIEDGLDQNDWAGWK
                     SLTEKLGSKIQLVGDDLFVTNIQKLQKGISEGICNSILIKVNQIGSLTETLAAIEMAK
                     RAGYTAVVSHRSGETEDTTISHIAVATNSGQIKTGSLSRTDRIAKYNELLRIEEDLGT
                     SAIYKGKKTFYNLHVK"
BASE COUNT         9185 a         4449 c         5034 g         9263 t
ORIGIN      
        1 agctaccatt gcaaccacca atgacgttag tggatattat aaagtattta tttgtatcaa
       61 aagcttttcc gggaccgata taaccatccc accaaccagg cttttcggct cctgcatggt
      121 agcctgctgc atgcgcggtt cctgagagag catggcagac gagtattaca ttgtttttgt
      181 ttggatttaa gactccgtag gtttcatatg cgatttcgag tgggctaatg acttgcccac
      241 tttcgagtgt taaatcggga agcttcgcaa tttttgtttc aacaattcct acggaatctt
      301 ttaaattcat agattacact ttttttaaag cttcgtccaa gtcagtaatt aaatcatcaa
      361 tgtgttctaa accaacagag agacgaataa aatcaggcgt tacaccagca gatctttgtt
      421 cttcttctgt caattgttga tgagttgtag aggcaggatg aattgcaaga gactttgcat
      481 ctccaacgtt tgcaagaaga gagaagattt ctaatccatc tataaatttc tttgcttctg
      541 gaactccacc tttgactcca aatccaatga tagcaccgaa aagatttctc ttgtggtatt
      601 ttttagcaag agagtagttt ttatcagaag gaagacctgg ataattaacc cagcttactt
      661 ttggatgttt acttaggaat tcagctactt ttaaggcgtt agacgaatga cgttccatac
      721 gaattggaag tgtttcaatc ccttgtaaga tttgccatgc attgaatgga gaaattgcag
      781 gaccgatatc acgtaatcct tggactcttg ctttgatgat atacgcaata ttcgctccac
      841 caaatggttc aaattttcca aacacatccc agaattttaa gccatgataa cttggatctg
      901 gttctgtgaa gtttttgaat tttccatttc cgtaattgaa tttaccagca tcaataatga
      961 tcccaccgat agatgttcca tgaccaccta agaattttgt gagggaatga actacaacat
     1021 ccgctccatg ctcaattggg tttactaaat aaggagaagg aagagtgtta tcaacgataa
     1081 acggaacacc atgatcatgt gcaatttttg caatcgcttc taagtctagt gtatctaatt
     1141 ttggatttcc taaagtctct gcatacacag cgcgtgtctt gtcgttgaaa tattttttga
     1201 aattgtttgg atcagatgga tctgcaaaat gaactttgat tccaagcttt ggaaatgtat
     1261 aatggagtag gttgtaagtt cctccataga gagaagagga agcaacaatt tcttgtcctg
     1321 cttctacaat atttaaaagg gcaagagttt ctgctgattg tccagatgca gtggctaacg
     1381 ctgctatgcc gccttcgagt tctgctactc ttttttctaa gacgtcagtg gttggattca
     1441 ttaaacgcgt ataaatattt ccgaattctt gtaatccaaa aagtctagct gcatggtctg
     1501 tatctttaaa tacataggat gttgtttggt aaagagggat tgctctagaa gtagtagcag
     1561 gatcgccctt ggtatagcct gcatggagtg caattgtctc cggtttgaat tgtcttgcca
     1621 taataaatgc tcctgttcgt tataaattga ataaatatac aataaaacgg atgcttcttt
     1681 ttaagtcaaa tagaatattc taataagttg gatggatggt ctgttatatt ggatggatta
     1741 aaagattacc acggataaac accgatggac accgatgttt ggaatcgata aaggctcatc
     1801 tcgactacca ctcgatactg tatcactaag tttagagcat tggtggttta taactttttt
     1861 ctttttaaac ggatacattg gtcatgcatt tagtttttgt atttacttta aatagaattt
     1921 ttttagactg aaggcatgat agtttcaatg aatgaaattc taaaactgag tgtcgtagga
     1981 aaatgaaatc gaaccagacg cacaaaatga gggttagcac ctaacggtta gtttgctgcc
     2041 gcaggctcat aaatttaaat ggataatgca gataaaccgc aaagtcaaaa ctcctttcta
     2101 aaaaaaatcc aacttctttg gtatattctt tggcgggttt ttttatcatt ggatatgtta
     2161 ttattttgtt aatattttta ctatttatat atacaacagt attctacacg tctgtgcact
     2221 atacaggtat cccaagttta aaactgaaaa aagactctca agaattacaa attgctttag
     2281 agaagtatgc agatagttat ggaaaattcc cgaccgaaga acagggatta tccgctctag
     2341 ttgaaaaacc aactctacca cctataccag aaaattatga acccattcta aataaaaaat
     2401 cattcattct tgatccttgg aaaactccct acattttaaa aatttttcca aatggagact
     2461 atgcaattat tacattaagc aaagataaaa aagtaggtgg agaaggaaaa aattctgatt
     2521 ttaatattct aaaagaagaa gaatatccag aatattttaa aaggcatcgt taaaagaaaa
     2581 aacccctgac tgaagtcagg ggtaggggtt aggagtaaat ttatttcttt tcgattagtt
     2641 tcaattcctc taagttgcgt aagtctttga ttctactttc cggatagccc aattcttcaa
     2701 aagccatcac acctttttct tttgaggtat ggcaactctc gcatttcatt gcttcttttg
     2761 tgatagagtg attgatcata agggttgcat cttgtctcat ccattttggt tcaactttgc
     2821 cactaaatgg aatggtccaa cctttctcaa ttcccatgaa atacatgaat ttatccatca
     2881 tatagtattt gaacatattt ccatacatga gtttgatgag tggatcgtct acagctttca
     2941 tcacagcgtc ttttggtttg cccgtttcat aatagacatt gtagtcaaat ggtaaaagca
     3001 ttccaccaaa cggaccttgg tttcccatgt cttcaaacat ttttgcattg aatcgtttga
     3061 atggggttaa cttagatttg ccttgactag cgattggtct aaatgttttc tcataataag
     3121 aatcgtaatc gaaattctta taaggctcat caggatttgt tttaaatgct ttgaatgttt
     3181 tcagtccatt cggattgtcg cctaatgcac cagccataaa agttccagaa ccattgaacc
     3241 atttgtaaat gattgcctct cctggttttc cagaacggag tatgtctttg taaatccaaa
     3301 ctccttcttc ttcgctataa actggttctg tccaatctct aaggactacg ttttcatccg
     3361 ttaggtgaga gatatgacaa gtctcacatg ctaatttatc tgtatgtgcg tttaagaaag
     3421 ctctttgatt tttgttttga atatgaggag aagaagagtg acatttttca caagttacct
     3481 ctacattagg taagtcgtta ctgactaaat cagttcctga ttttccacga gcaattaaat
     3541 gtccatgggt ttcatgacaa tcaatacaat ttataccggc tctatagtga acgtctatgc
     3601 cagttgttgg agttccccgc ttcgctcccg ggtgaaggag tcttggattt ttatatccta
     3661 aattcattgc tgctttattt ccaggataca tatctccacc atggttgtgc tcatgacaac
     3721 gtaagcaatt gtctgaattt ggtttgattg cagccatcgc cgcttttaag cttctatctt
     3781 gattccatct atattttcca ttttcatctt gcacaacgta tttctgattc atatcgtaag
     3841 cagcagagtg acacattaaa caatctgtgg attcaaattc aaaactctta ggtttatatc
     3901 caggaaacat agttccactg ataggaccat attgcccacc agcatgacat tgaccgcaac
     3961 catcacttct aggaccatgc tttgtattaa cgatagtcgc ccaacctgtc catgtgaagg
     4021 agccaggtat accgcaagct ctatcaattt ttccaagagg aattcctttt actctttctc
     4081 cgttgaatcc gtaggtatta aaaccttcaa atttgtttaa agaaaaatgg atgctagatt
     4141 caatatttgc ccttgtgttt acagtgtcat atccaccatg atcgttttta actttcatag
     4201 tctcatgaca acttaaacag gtttcaggac ctttgtattc ccgaattcct gcctttctaa
     4261 aacgatctat atgaacaagt ccagtgtttc tctgaaaggt cgaaacttta tctgatagat
     4321 ttttagcaac tgcatcatag actgctttgt tttgaatcga tttatctact gatttgtaat
     4381 tactaaactc gtcctgtaat tgttttgcta tatcctcaaa ttcagcatcc gttactttga
     4441 tcgttggata ggaaatcttt tccgaacgat gcacataagg aatgatttga ttgatgatat
     4501 ttgaataatt caaatacaca actccaaaga agattatcca aaataggata attcgtattg
     4561 cccatttctt tttttttgag agttccatga ctttactcct taattttatt tccagtaatt
     4621 ctttcatact ctaacggatg ttctactttc atctcttctt ccgttagttt tccatcaatc
     4681 caaacagtgc ttcctggaaa tttatctgga ttataatgca cattataaaa atgccagaca
     4741 acaattgcag aaatagctag gattgcctca tcgctatgag caatatacgc cataggtatt
     4801 gcaatatttg ggagcatgtt tccaaaaaaa actggaaacc acaagataag ccctgagaac
     4861 atcataacag gaactcccca aaaaactgca aagtaatcaa atttttctct aaaattaaat
     4921 ctatcgtata acggtaattt ttcttttaaa ccaaaataat aaagggaggt ttcccaccaa
     4981 tcttttacat cttttaaatt aggaatcatt ggccaagctt tacttacatt aaagcggttt
     5041 attacgagta ggcgaaatat ataatagagg tgatagagtc caagtagaat cataatgaca
     5101 gcagagactc tatggacaat ccttgccccc tgtaagccac cgaagaaaaa gtaaatatat
     5161 ttcacccaac taacgtccgg aaatcgcatt ggaattccgg tgatactcaa aagagtaaaa
     5221 cttagaatca taaagccatg ttgaattctt tgctctaaac tgaggcgtag gaattttttt
     5281 tctgagtttt cctttttcat ataattctcc ttttaatcat aaagcaaagc ttttaaaagt
     5341 tttaagactt tttttagaac agttataaat agaagaaatc cttcttttat aaaagctcca
     5401 agtttttcaa agacagaaaa ccaaccaaat ttcttttgaa tatcaagtat gattccaacc
     5461 acaagcattg ccatcgttcc cccggttaga atttgaaaca tcttttcttc ccaatataat
     5521 ataggttctt tttccactct taattttaaa tgatttgctc cactcatagc aaagttcagt
     5581 tttactcctt catgacaacc tgattgacca caagtatcgc taactttatt cggatggata
     5641 ctagcttctt ggttgtcttt cggaagtatt ttatggactg catgacagtc ttgacaagtg
     5701 gcagaactga ttcctccaaa ttttacagct ttgtaatgaa aactttgtct gtaggattct
     5761 actgcgtctg cttctacttt atttcgaatc atcatttctt tgttgtcatg gcaaacaata
     5821 cactgtgaaa ttttttctcc ttgcgtgatt gtctttgtaa cctttttaat attatgaaca
     5881 tttgtatatc catgacaatt gatacaggta ggactatcag gattggattt tattttccct
     5941 tttgtatgaa tcgagtcatt gaattcattt tcttgtttag aatgagtata agtcttacct
     6001 tctttgtcaa tcgaatgaca atctgagttg caaccaactt taggtaaatc agtgttatgc
     6061 ggataagact ttgtttcttt atgacaatca atacaattta gttttccatg tgcagagttt
     6121 ttaaattctt ctgtgtttac tgttaaattt ttcaaagatt ttaaatcttc gttcaaatag
     6181 gaaaagttat tcattgcatg gcatttatta cagttttgct gtaaatcatc tgaatgaatt
     6241 cccaaggtaa aaaaaagaaa aataaaaatg aaacttacat aaataaactt ctttttttcc
     6301 aatggaattt gcttggtaag gaaagatttc atatactata ccctatatgg tatattttat
     6361 ttaaattgta attaaaaaag caagtcattt aaaaaaaatg ttaaatctat gatctataga
     6421 aataattcta agtttacgct aataaaatca tcccatatga gacaaacaat catttaaata
     6481 gagataacaa ggatttgtct atagaaggat caaaattcag aaatgataat ttaaaaacac
     6541 ctttaattta attcgataag attttcttgt gatgaacaat gaatactttt gatttctctt
     6601 ctatttcttt gctttgtaaa acgggaaaag cccttttact tcttaggtgg aaagattttt
     6661 taggatttca gacttttctt ctcttctcaa tttcctgcaa aatagagaaa aactgttaaa
     6721 aaaggggatc tagggttgtt ttcagattcc aattcattct ttctaagcta ataattctaa
     6781 aaagactctg ggtaggactt ataaacggat cagtgattct ttcgaaaatt tataaattga
     6841 caaaaatgac atacgttcat aaaatggata tagttcaatt aggagtcagt atgtcagaaa
     6901 aaacatatag aaaagtatat aaatggggag attcagcaaa agaagagact ttaaaccatc
     6961 acacagctga atttttggag aagaacttct tcatacaggt caaaaaggag ccttatttgc
     7021 ctggaaacac agaagtgtcc ataaaaaaga aatcctctct ttctaaagct gtgaaagaca
     7081 aattgatttc aattgtggga agtgaaaact tctatgactc cgactttgaa cgagcaagac
     7141 atagcattgg aagattttat acggaaatcc taaaagcgcg acttggtcag attgaaaatg
     7201 caccagatgc agttgttgtt ccgagaaatc aggaagaagt agcaaagatt gtagaagttt
     7261 gcaatacaaa taaaattcct atcattccct ttggtggaaa ttcatcagtt accaaagctt
     7321 tacaagctcc gaaaggggga atttcgctcg atctgaaaaa tttaaataaa attttagact
     7381 tcaatgaaac caattgcact gtcacagcag aatcaggagt ctttggtcct gttttagaaa
     7441 agtttttaaa cgatagagga tatacctgtg ggcattttcc tcagtctttc gaattttcta
     7501 ctgttggagg ctgggttgct gcaaaaggag cagggcaagc ttctactggt tatggaaaaa
     7561 tggaagatat tcttttgggg ctaacagcca ttaccccaaa tggaaagttg gagtctaaaa
     7621 tttatccagc agcctcaatt ggtccggatc tttttaaatt ctttttggga tcagaaggaa
     7681 tctttggagt gattacaact gttacattga aaattagaaa gtatgatcct aaaaattctt
     7741 ctatgacttc atttatcttt cctgattttg aatcgggtgt aaatgcaatg cgtgaaatca
     7801 tgcaaggtgg atttggaaag cctcatttct tccgtttgca agacccagaa gaaacagaca
     7861 ttgcttttag aatgtctgga aaagaaggaa gtatttccga taaatttcta cggctaatga
     7921 agtttacccc catgaagaga tctttgatgt atgtgattgt agacggtgat cctgattaca
     7981 caaaattttt aattcgcaaa gtgcgtaaaa tcgctaaaaa aaatggagcc ttcgaagttg
     8041 gaaaatctcc tgttcacaaa tggctagaac aaagatattc cagcgcgtat ttaagagata
     8101 cattaatgga tcaagctgtc atgattgata cacttgagac tgcagttact tgggacaaat
     8161 tattgattct ttggaaagca gctcgggact atattaaaag ccgcccacaa actttgtctc
     8221 ttgttcatat ctctcacgct tatgaaaatg gagccaattt gtattttata ttcatgagtc
     8281 caatgcaaaa agaaaaagag ctagaagagt ttaataaatt ccattcaggg ttaatcgata
     8341 ttatccacaa aaatcatgga tctctttccc atcaccatgg aatcggaaga ttgacttctc
     8401 atcttatgaa agaagaagtt ggcggttttg gattagaagt tttacaggga ttaaaaaaat
     8461 gctttgatgc aaaggggatt atgaatccgg gaggaaccct tggtcttaaa tgatttaaat
     8521 aaaccccaaa gtcgaaaaga gagagagttt ataaaaagag gtgaggagat tttagaagtc
     8581 gcaaaaactc tttttaaaaa acaaaatcca gctctggtga gtatggatat gatagcacaa
     8641 gcagttggaa ttggaagagg gacactctat cttcattata aaggcaaaga tgaactcatg
     8701 attcgaatcg tcctaaatcg gcataaggaa ttgctttcag atttagaaaa gatggatcta
     8761 tcgcttcctg gtgaggagct tgctaggaaa gcaattcgag tttatcttga gcataacttg
     8821 aaacaaccag aagaatatct catgcaaaag aaatgtgaag aaaatattat caaagaaaat
     8881 gcaggagaag aaattctaaa agaagtagag gaagaaaggc tgaagagaat tgcaatcatt
     8941 gagaaagttt ttattaaact caaatcagag ggaatcatac gagatatccc tccttatctt
     9001 ttagcaggag cgatatgggg aatgctaaaa gggactcttg atattattgt tggaggtcac
     9061 ttcaaacaag aaatcgaaga cgaagaaatg ttttttcagt tagtagaaaa tttactattc
     9121 tatggtcttt ttaagaccaa ggagaaatag aaatgaatac aaatcatttg agaaaaaacc
     9181 gactccaatt aaaagatcta agcactgaaa tgtttgatgt tgtagtgatt ggtggcggta
     9241 ttactggagc ctgtatcttt tgggacgcaa cacttagagg gatgaaatgt cttttggtcg
     9301 aaaaaaatga ttatgcttcc ggaactagtc aagcgacttc taagttgatt catggtggac
     9361 ttagatattt aaaaaattta gaaatcggtc ttgtaagaga gtcgcttcga gaaagaagaa
     9421 acttagctcg tatttcgcct cacgcactct atacaatggc ttttcaaatt ccagtttaca
     9481 atacaaaaga tgttctaatg acaaagtttg gaatgatgct ttacaatcaa ttgtcctatg
     9541 atcgaaataa attgattcat acagacaatt taattcctga atatagattt cacaatagag
     9601 aagaaacaat ttatgaagtt ccagaaattc cacgcgacca tctgaaaggt tcttacctct
     9661 actatgacta tgcgaactta aatccagaac gttatacaac agagtttatt ttttccgcaa
     9721 gaagccaagg tggaatttgc agaaattaca ccgaggtaat gcatttagaa aaaaatggaa
     9781 atggaattta taaaatacaa ttaaaagata atattaccgg agaagtgggt ttgatcgaaa
     9841 cgaagacggt agtgaattcg acaggtcctt gggctgacaa attagaatcg atgatgtttt
     9901 caggaaaaga gacaaagctt gttcgctcta aggggataca tgttgttacc cgaaagattt
     9961 gtaaggacac tacagttgtt ttaaagaaaa gaaatggaac tcatcttttt gtaattcctt
    10021 ggagaaataa aacaatcata ggaactacag atacagttta ttctgattcg ccagatgtat
    10081 ttaaagttac acaaaaggat attttggaac tactcgagga ggtaaattac gcatatggat
    10141 cgattcaatt aaaattagaa gatgtagatt tttactatgg tggacttaga ccactcgtag
    10201 aacaggaagg aaagaataca tacaatgcct ctcgtaaact agaaatcatg gatcataaag
    10261 aagaaggttt tgctggattt tattctgcac tcggcggaaa atatacaaca agtagacttc
    10321 ttgcagagaa gttagtggat aaaattgcag aatatctccc aaagaatttt tccgaatgta
    10381 aaacagagac tactcccatt ctgggaggga atttttctga cttcaaaaca ctcgaacaag
    10441 aattacaaaa agaatttcca aaagagtcgg gtgaaaaaat agaaatttta gttcatagat
    10501 atggaagcga ggcaagaact attttaaaaa cagaaattga agaaaaagaa atctttcatt
    10561 tgcaaaatgg agagcatatt tattccgaag agattgcgca tattgcattg aaagaagata
    10621 tagtttatgc tagtgatttc tatttcagaa gatcgggtgt cggtgttccg ggtaaaccag
    10681 atccgatgaa taatgataaa atattaaata cattaggaaa gtatttaaat tgggataaat
    10741 ctaaattagc cgctgagaag aattgtatag agaatcgtta taaaataata gcttaattta
    10801 attagtataa gatttgaaaa ttctagatag gagtctaaaa aatgatctag aatttatcct
    10861 ttaatatgaa aaattctttt taattttata caattaacat cacaaaccta tatcaactat
    10921 cggaaacggt agactatgcc aaataactta gaatgtgtgc tgtatgatta agattaatcc
    10981 ttaaaatcga ttgacaatct ttatacctgc tgtttttatc tcctcgtcta gtgaaaatta
    11041 tgaaaaagaa aatacttata gtcgatgatt ccgaaactag ttccatgttt ttgaaaatgg
    11101 ctctacaaaa ctatgatgta aattcagtta ctagtggaag agaaatgtgg aaactactag
    11161 aaagtgaaat tccagattta attttaatgg atatcgttat gcctgaggag gatggatact
    11221 ctctttctca aaagcttctt ttgaatgatc aatataaaga cattccaatc attttccaaa
    11281 caactaaaaa ttcaagtgat gatcttgaaa aagcatttga tacaggtgct gttgactata
    11341 taagtaaacc gatagacgag aaagaattaa gagctagaat taaatcagct cttaaaatta
    11401 aagaattaga aaaagaatta aattcagctc ttactgttat taaacaggat ttagcagttg
    11461 ccgcaaaaat tcaaaataaa attattccag atgacgttca agtgcgaaat ggtttagttt
    11521 tttatactaa atatcttcca atggtagaag tcggtggaga tttttattat ataactgaaa
    11581 ttggatcggg tattactcga atctttctag cggatgctac tggacacggt gttcagagtg
    11641 cgttaatcac tatgttgata aaaagtgaat atgaaaatct agcaaaaaga tctgaatcac
    11701 catcagaact tttgtatcat ttgaatgaaa cattcattaa taaataccaa gtgctaaaga
    11761 cttttttcag tgcttttgtt gtagatgtga atataaattt aaatactcta acctacgcct
    11821 ctggtggaca tccagagcag gttttggttt ccggtaaaac aaataccatt gaacttctta
    11881 aaaagacagg aagacttgta ggagttgttc ctgaaaccaa atacaaagaa attacaatgg
    11941 agttcgtaaa aggggataga ctctatattt tcacagatgg actctatgag gagttcaatg
    12001 agaaagagga aatttatgga gaagaagagt tatttgatat tctccaaaat aacaatcgaa
    12061 atcctattaa gcagatcata gagtttattc tctccgacct aaaagacttt atgggaacaa
    12121 gacaacagga tgatgatatt actgtaattg gtcttgagat agcaccttga ttgtttcttt
    12181 tttaaatgga agagcatttt ctgtttcatt ggaatcctga aacaattcat tcgattcatt
    12241 tacatactgc atagacaatt cataaaatcg gatccggtct gtttcgtttt tttccatctt
    12301 gatatttttt ataaaatagg cattttcttt taagaccggt ggagtaatga tagagcgaga
    12361 ttttagtttc tttgcggaat ccgtctgact gtttttattt gcttccatct cttcatattc
    12421 atatctaagt agaatttttc taatttctaa attatctttg tcgtagagtt ttattactaa
    12481 cgttcgaaac aaatctccag ttggaaaagc atgacccaat cttttcgctt ctagtataat
    12541 tttgatttct gtttcctcta ttttatataa ctgaagggaa aaggttcttt ctagatagtc
    12601 tttgctatgt cctccaggga aagtatgggt tctgtaatca ctaattgttt gcatatgaca
    12661 atcctgacag ttttcttttc catagaaata gctcatttgc cattcgttga aggtattttg
    12721 cattggttgt cttgaaaatt tgaattctct atgtggaact tttcctgttc cagttgggaa
    12781 attgaattga tgacaatttt cacaaaactc tgatttcttc attgatttta cttctaaata
    12841 agaatgttcc tttgggtttt gtggaacgtg tgcggttaaa atcttatctc ctcttttatg
    12901 gcaaacgata cacgatacac cttcctctat aaaaaccctg tctcccaagt tttccagatt
    12961 tccaccagat ttaagaagag gagcatggca gttaacgcac catacaagag gttctcttct
    13021 atggctttct ctatataaat catttgtaaa agcaactttg tgcctgcttg tttcccattc
    13081 tctataaatc tctttatgac atttttgaca ttgctttgag ctttctatat cagattctac
    13141 ttctattgca gtaagacctt tattaaaacc atgaatattt ttttgcttac attgtgttag
    13201 aaagagagcg gctaataggg tgaatcctat gtaagatact agatgaatgc gcataagaaa
    13261 tcagtttagt tgctatattc gaaacgtaaa ttcatttttt aattgattca ttagtaatct
    13321 attagagatt cctttagata ttttcattca atagaattgg aattgcaatg aatttttcag
    13381 tttataaaaa agattttgct ttacagaaat aaaaaatgat tcaaagtaac atgccgattt
    13441 tttagttatt cgtgtcaaat tgatttatat tattttgcgt ataacaaggg gtaaccgtgg
    13501 aactttttca aaatttttat tttaactatt atactattgg tgtcataaca ggtttattta
    13561 tgacaggtct tatcgctttg tttttgtctc ttcttcccaa aaaatcaaca cctaccaaat
    13621 acttagtttt tatctatctg acaggagcat tagtgagtat tggctatgct ctagtgcaag
    13681 gaatttacct tcccacaaaa tggataagga tttatttatt aattgcaatt accttaaatc
    13741 attggctttc gattcaatta tttttccaat ttccggaaaa caattatcct aaaacagcaa
    13801 aaggtttttt atactcacaa ggaataatat tttttctatt agattctttt ttaatttata
    13861 aagcaatgaa ctctggagtt gtttatgatt tttccggtca ttactatgag cttgattcgc
    13921 ctatcgcatt aaagatctac ggattaatcg tccttgtgaa tatttttgta atcactggtg
    13981 tttcaatttg gagagtaata aaaacgaaga cagatgaaaa attagctata gcttttatag
    14041 cattaggatt tggtttagct ttttttgttc cagggctaat taatgtttta aacaaatcgg
    14101 gtattgtctc tagaactgtt tatattacta tttattgtat gtctacagtt gtaggactat
    14161 ttatggtgat tgtagcttat attaataaga caaaagacaa tacaacattc atgtttaaaa
    14221 ttgttggtat tagttttgta gtctttatgg tcgtctatga tatctttgca tttattgtaa
    14281 tgaaggaagt ggagacaacc tatgatatta ttcacaacca ctctacaatt cttgcaatgg
    14341 aagcaaaaca tagacccgat gatttaaagt atgttgttga atatgatcca gaaaaagata
    14401 catttacata tattcaaaat acggataata ttgagttgcc ggaaaatttt aaatatgatt
    14461 taattaattc ctatgttttt gattctttta gtaaatctaa agaagcatcg ctaattgaaa
    14521 tgaaacaaat cctttccaat ttgaaaatca aagattctga gtatttaagt ggttatgcta
    14581 gtttgattaa tgaatattta gaaaaatttg aaccagaaac tggagtgtca aaggcagatg
    14641 ttaactcatt gattctaaac aaagaaagat ttttatttat tcgatccaat aaagtagatc
    14701 aattagatga gaaagatttc agaaagaatt tggagtcttt attacagaaa gagaaagaag
    14761 attttttacc atttaaacag gctatcgaaa aacatttagc aaattcaaac aaagatggta
    14821 ttgaacttaa aaaagaaatt ttaaatttct tagttccgat gcaagatact tcgctgcgaa
    14881 gatatcgtaa gagtttagac ttaaaaaacc attatgttac atttcattat attgatagag
    14941 agaaaaataa aatttatgaa atcggatact cttatttaga atacagaaag tttattcatg
    15001 cagtcgctgt taaattgatt tatatccttt tatttgcgtt agttcttatg gtcattggta
    15061 tccccatttt tctttcagga acattgatta atcctttgaa cgcacttcta gaaggactgg
    15121 gtaaagttaa aaaagggaat ttagatataa agattccagt aaaagttcag gatgaaattg
    15181 gttttctctc caattctttt aataatatgg ttcaatctat taaagactca aaagaaaaat
    15241 tggaagagta tgctgagcat ctagaagaaa aagtggaaga gagaacgaag gaattgcagg
    15301 ttagtttaaa tcaagtagaa aaactaaaaa gccaacaaga tggagattat ttcctaacaa
    15361 cacttttact caaacccctt ggtgtaaata aagtccatgg atctaaaatc aaggtagatt
    15421 tttttgtgaa gcaaaaaaaa gaatttaagt ttagagcaca tgaacatgat attggtggag
    15481 atatatgtat aacgcatcaa gtcaaactta aaggaagaga ctacataacg tttttaaatg
    15541 cagatgcaat gggaaagtct atgcaagggg caggtggagt gctggtctta ggtgctgttt
    15601 ttcaatctat tattcaaaga actgtttcgt atcgagctca ttcagaaatt ccaccagaac
    15661 tttggattaa aagagcattt aaggagatgc ataaaatttt tgaaagtttt gatggatcaa
    15721 tgctcatctc acttgttttt ggtttagtcg atgaaaaatc tgggcatgta tattttatca
    15781 atgcagaaca tccttgggtg attttatatc gagatggtgt agcggatttt attgaacatg
    15841 atatgaagtt tagaaaattg ggaacaagtg gattagataa cgagcttgcg atttctacct
    15901 ttcagatgat gccgggagat tttttaatta ttggatcaga tggaaaagat gatttgatcc
    15961 tttcgaaaga cgttaagagc ggcacgagag taatcaacga agatgaaaag ctttttttaa
    16021 gaagagtgga agaagcaaat ggagatttaa ataaaatatt tgaactcatt acaaataaat
    16081 ttgaactcat ggatgacttt tctttgcttt ctattagcta tccaaatgaa gaaatcgaat
    16141 taaatgagga tgaggcaaat caaatagcgg ttactcttgg taatgcgcga aagcttgtag
    16201 gagaacataa atttaaagaa gcgatagaaa tattagaagt atcgtatgaa caaaatccaa
    16261 atcgtttgga agtggcaaga tatttgatta aagcatatat gaaacaaaga agatacaagg
    16321 aagccgcgag agtctgcaaa gagtatctag ataaaaatga attggattcg agtttaatgt
    16381 ttaaagcctc ctattgctta aaaatgaatc atgaatttaa agaagcgatt gagatggcag
    16441 aaagaattaa attgagagaa cctctgaata tacgaaattt aattcattta gcagatatgt
    16501 atacctataa taaaaattac gatcggtctt ataagctact tagaaaaatc ttaatgttag
    16561 aaccagagaa taaaattgct gaaaaaatat tagagagagt agaacaagaa agaaaactag
    16621 tcgtttaatt tgtcttagcc ggtggactag gtaagagttt taatctttcc tgtttcacct
    16681 tttcgatttc tgttcttata ttttgtcctc ttcttgctat atatagaagt ataaagttaa
    16741 aagaagtcat gcttccgtct ttaaaagaac aaaagtcttc tagttcaatt tctcttccgt
    16801 ttgggttata ctcattagcc tcagtctcaa ctgtcccacc ggattcagag cagaccaaaa
    16861 aagcaatttg ctttgggcta tctcctttac taaaaaaccc tttttctcgg atgagtggaa
    16921 ctaatttacc tgtttttata tactcataag gtttccaagc tccgcactcc cttccttttc
    16981 ttccgcttcc cgatttataa atacagaaag aactaatcat tacacccgct tgcggaaatc
    17041 tagtaaaaga atgcctaggt aatttttcat ctgggaattt gtaagtaaca tattgcggtt
    17101 tttcataaaa atcattctcc caactcaata tgggaatgat agaaagataa atcgcaagga
    17161 agattaaaat atgttttaaa ggcataacta caattaagta aatataaatt aaattacaat
    17221 cattaattca aaacaaagcg gataatacta gtgtgcaatt ttgagaaagg attttcatag
    17281 aattagaaaa tgcaagagtg atttgaagta tattgacaat tctacacttt ttatgagact
    17341 gacttcgagg tgacgaatgc gaaaggtcat agagtatttt ctttctaaaa gtcttttatt
    17401 aaatttatta acagttctga tcattctagt tggatcttat aaagctttga cgatgaatcg
    17461 agaagctttt ccaaatatca attttgatat tgttacagtt acaacaattt atccaggagc
    17521 atctcctgcc gaagtggaaa aattagttac aaaaccaata gaagacagta tcaagtcggt
    17581 tgatggaatc aaagaatatc gctctgcctc cattgaaaat agatctggaa tcgtaatcac
    17641 cattgaccca aatgcaccag atactcagaa agtagtagat gatataaaat cggcagttga
    17701 ccgaacagaa gatttaccgg aggattcaaa gaaaccgatt gtgcaagaag ttacctcaag
    17761 cagaaatcct gttatagaaa tcaatgttgg ggtaaaggcg gatgcaagcg gtaaaccagt
    17821 attaaccgaa aagcaattca aagaaaaagc aaaacagtta gaagatttgc ttctagatgt
    17881 tccagaagtt gggcgtgttg tgagaagggg atatagagaa gcagaaatgc atgttgatat
    17941 taatccgttg gctctagatc gtatatcgct ttctacaaac cagatcatca tggctttaaa
    18001 aggaaaaaat gtgaacttcc caggtggaac aatcgcagat gcaggacggg aagcaatcgt
    18061 tcgaacagtt ggagaatttg ataccgaatc tgaaattgaa aaggtattta ttcgctcaaa
    18121 cgatgcaggt cgttctatta tattaaagga tgttgcaaag gtaacagaag atttcgaaga
    18181 taagcaatac atcgaaaaag taaatggtct ttactctatc gttcttgtag tcgtgaagaa
    18241 agaaagagga gatgcaatca aattagtaga caaagttaaa aaagttgtaa ctgattatgg
    18301 agccactcaa tctgattata taacactttc gtatgtaaat gatttatcta aatacattcg
    18361 tcgtcgtttg gatgttttag tttccaacgg tttgcaaggt tttgttttag ttgtaatctc
    18421 tctctttttc tttttgggat ggcgtgttgc cctcatggta gcactcgggc ttccgcttgc
    18481 tatggcgcta acgtttatta ttttagatta tatgggtatg actctaaatc taatttcgat
    18541 gttaggactt atcattgttg ttgggatgct cgtggatgat gcaattgtga ttagtgaaaa
    18601 catttaccgt tatttagaag aaggggtaga gccttatgaa gcttgtattc gtggaactat
    18661 agaagtaatc gcacctgtaa cagcaacgat tacaactacg atagccgcat ttggtccaat
    18721 gctttttgta acgggtatat ttggtaagtt tatctattcg attccgatgg ttgtaattat
    18781 tgcattgtcc tcttcacttt tcgaagcatt ttttatttta ccatcccaca tttatgatgt
    18841 aaacaagcat ggaattaaaa aaggagaaat aaaagaagaa ggtcattggt ttgtaaaagt
    18901 aagagaaaga ttttatagac caagtctctc ttgggcattg aagcataagg gtttaactgt
    18961 tctaatctta gtaacagttt ttgcattttg tattgggtta aatgcagcct ttggtaagtt
    19021 taaattattt cctggtggaa ttgaagtatt catagtaaaa atcagtgcag aaacaggtct
    19081 cacattagaa gaaacagaaa aatttgcaag agcagtagag agagagatag cactccttcc
    19141 acaataccaa gaaaagaaag ccaatggatt tgtagagaga atacaatatt tcttccattc
    19201 attgggactt gtaattcaac caaagtttca tacgggagaa gtagaaaact atgtgactcg
    19261 agttggcatt attcagaagg atgtgaacga tccttttact aagagaggaa aaaactttgc
    19321 tcaggttgta gtatatctta cgcctgacgc agaaagaaag agaagcacag aacaaattat
    19381 aaactatgtg agaaaaagaa cagaatggct tatgaacgaa aaagcattag ccgttgcaaa
    19441 acaaaaagag ctagctcgta gaaatgcaga aaagaaaaaa aataaaccga gtgatgaaga
    19501 aaatataccc attgaaattc ctgaagaatt taaagaactg aaagggaagc tagtatcatt
    19561 agatctagag gcaattcaag ggggacctcc tgttggaaaa cctgttgcga ttgaaattag
    19621 aggagatgat tttacaacac tcaaaaagat tgcacaagat tttaaagatg tgatgggaca
    19681 ggtagaaggt gtcattgaca taggggatga tttcaatgat ggaaaggatg aaattcgtct
    19741 aacgatcgat gaagctctag cagcgcaggc aggtgtctcg gttcaacaga ttgcacttgc
    19801 ggtaaatact gcctaccaag gaactgttgc aactacgatt aaaagagcag atgaagaggt
    19861 tgatgttcga gttcgctttc ctgaaaattt tagaacgtca atccgctctc tagataaaat
    19921 atttgttaat aataatatgg gaaatctaat tccaatatct agaatgacaa agtatgaaaa
    19981 agttcctggt cttgcttcta tcaaccactt agatggaaaa agattattaa ccgcaacagc
    20041 caatctagat gaaaccaaaa ctacttccat gaaggccaac caagaaatta aaaaattagc
    20101 agacgcagct aagattatgg ataagtatcc tggttataga attcgttatg ggggagaaaa
    20161 taaagatacg gaagaatcat tggcaagttt aggtagatcc tttatgattg ctttctttat
    20221 tattttcatt attttggtat ctctctttag atcttttgtt caacctttga ttgttgcatc
    20281 tgcaattcct ttttctttta tcggagtcat cattgcattc gtgacacatg gacagtattt
    20341 tagttttttg agttttatag gaattgtagg tctatcgggt atcgttgtaa atgactccat
    20401 agtattagtc gattttgcaa accaattgag aagccaaaga ccagatatgt catcttatga
    20461 tattctatta gagactggat gtgtgaggtt acgacctgtt attcttacaa ccgttacaac
    20521 tgttcttgga attttaccaa ctgcatatgg aattggagga tcagatccat ttttaatgcc
    20581 tatggccctt gccattggat ggggacttgc ttttgcaact ttcttaacat taattattgt
    20641 tcccgttttt tataaaattg tgatggattt tcagatttgg tttagtgata aattttttag
    20701 aaaagatatt aagacattag cacaagagca agcttagttt aggcttctct tgtttaaaaa
    20761 aaggaattca aatgagttta aattgtggaa ttgtgggact gcctaacgtt ggtaagtcta
    20821 ctatttttaa tgcgttaact aaagcaggtg cgcaaatggc aaattatcca ttctgcacga
    20881 tagagccaaa taaaggaatt gtagaagttc ctgataaaag actagatcgt ttggcagagc
    20941 tttataatcc caaaaaagtt attccaactg taatggaatt tgtggatatt gccggacttg
    21001 taaagggtgc aagcacagga gaggggttag gaaataagtt tttatctcat attagagaag
    21061 tagatgcgat ttgtcatgtt gtaagagcct ttgaagatgc aaacataacg catgtgcatg
    21121 ggaaagttga tcctgtagaa gatgtgagta ttgtaaatta tgagttgatt ctatctgatt
    21181 tagaaagcct tgaaaagcag tatcctaaat tacaaaagct tggaaagaca aataaggaag
    21241 cacaagagtc tgcaactgtt atggagagaa ttataaatat tctcaaagaa ggtaagagtg
    21301 ctagactatt agatttgaat gcagatgaaa agaaaattgc tcagaagttt aatctaatta
    21361 cattgaaacc cgttctctat tgtgctaacg ttttagattc cgatatcggt aataaatcaa
    21421 atccactctt agaaaaaata tacgcaatgg caaaagaaga aaatgcagaa gttgtagttc
    21481 tttgtggaaa aatagaggaa gagatttctg gtcttgcgcc agaggagcaa atcgaatttc
    21541 taaaagatat tggagaaaca gaaagtggtc tcaatcgaat gattcgatct gcttataaac
    21601 ttttaaacct gataacattt ttaacagcgg gagagcaaga agtaagagca tggactacac
    21661 ttgcaggaag tcctgcacct aaagcggcag ccgtaatcca ctctgatttc gaaaagggtt
    21721 ttattcgagc tgaagtaatg gcgtttactg atattgatag acttggttct ccagtaaaag
    21781 tcaaagaaga aggtaaatta aggcttgaag gtaaagacta catagttcaa gatggggatg
    21841 taatctattt tagaatcaac gcttagtgac caatatagag gataatccta gttagcccct
    21901 agcttgattt aagagagggg cattgtcgtc tgaaaaatta tccatagcag atttagtcgc
    21961 cgaatctggc tcacgtgttt tagcatttat tgctcgatat acttcttatg tatttgctgt
    22021 ttccattttt tctgctggat tttcaggagc tgtaagcact gtattcactc tttattattt
    22081 tgatgctgta aatgtttctg tttctgaaac atttatgttc attttaaaag cacttatatt
    22141 cacttattgg ataattttaa tgatccaaca tggtcttctg ggtcgatttg ggattcctgg
    22201 cttcggtctt catattaaaa ccgttaaccg ctatgtaaaa tcaaaccctt ctattgaaat
    22261 ctcaaaagat ttatcagaag aggaatataa agaattattt aaatctattg ttgcactgcc
    22321 cggattctta gcattcaatc tttttatatg ggtatttgtt ttaattctaa tcgcaatgat
    22381 gcttggaatt tttttagagg gctttaatgg gagtgtaatt ttttcccttc tcacgattga
    22441 tatcatttgt ctttatgata cagtgtgtat cactctgatt gcaagtgaga taatgactgg
    22501 aagcattcga gaaaaatgca aagagattat gaattcgaaa ggaattcaat tcagagatag
    22561 agcactttct acagttctca caaaatttat tttattccta attcttttag cgattgcatt
    22621 gtatttatcc aatgtagtca cttattataa ccacgatcat atagaaaaag taattagttt
    22681 ctcgatttta gcattagtag tctcttgtct acttgcttat atgatatttc gaatcatcta
    22741 caatgcgctc aaacagattg aatcggctgc ttatgattta atgagaggtg gatctgggtt
    22801 gatctttcct agatcattag ataaagaatt tattaatgta gcaattggtc taaataaagc
    22861 atcaaaaact atcaatgatt ataaaattag tctggaagaa aaagttcaga aaagaacctc
    22921 cgagttaaat gcggctaata atgagttaat cgcaaaggga caaattgcag aacaagagtt
    22981 ggaacttgct agaaatatac aacagggaat tataccatct gatcttaaaa catggaatgg
    23041 aatttctttt gctgcttact atgagccaat gggaaaagta tctggagatt attatgatgt
    23101 attttatttc tctaagaaaa tcttcgtgct catggcagat gtctctggtc atggagttcc
    23161 cgcagctatg attacaatgg cagcaaagca ggcatttttg caagtgattc aagaagagaa
    23221 aaaaccggct gatattttta aagaagtgaa tcaaattatt ttagatcaag taaaaacaag
    23281 tgattatcta tcggcatttc ttcttcgtat tgatgagaag aatcgactaa cgtatggaaa
    23341 tgcagctcat ccacaggcaa ttcattacat tcattctaga gaagaatacc agcttttaga
    23401 tacagaggga atgtttattg gagcagtaga agaagcttct gatttttatg cgaatagtga
    23461 attaaaactt caaagcggtg accgaattta cctttatacg gatggagtat tagaacataa
    23521 aaatacttcg ggtgagcaat atggtatgga tcgatttata gagacattat cgcgaaatcg
    23581 aaatcaatct ctttcgattc agatcaatga agtaatttct agtctaaaga aatttatggg
    23641 caatgctcca atacgagatg atatatccat cattgcaatc gaattagatc ctaattgggc
    23701 aaagttcatt gatctattca atcaaggtct aaaaaactta agagaaaaga aattagaaga
    23761 ggctctcgct caattcttga atgctttaaa aattgttcca acatttctag cgattcaatt
    23821 tcaccttgct gcaatacatt ttcatttagg tgatctggat aaatcccttg aacatatatt
    23881 ggtctatcta aaagacaatc ctttagataa aaaagcaatt cagcttatta ttaatattta
    23941 ttctaatcaa ggaaatgaaa aagaaatgaa taaatggctt acgcagttta ataatattca
    24001 ttgatctaaa aaaaatttac catcgatagc caccgatggt aaaaatattt caaatagaga
    24061 tcatagagaa taaatcaact ttagtttaag ataagttttt aaacaaggga taattttttt
    24121 attggatctt gtttaaaaga agtgattaga attgctaaga tacaaatgtc catatatttc
    24181 tccttattgt ttgcgatata aaaacgactc aatcgttgtt tcctcaaatg cactcagaaa
    24241 cgaataaaat aattttaaga aaccaatgaa ttctttatct attgagagaa tgttgtatgt
    24301 tgttaacgtt aattcataat tggctacttc aatagcaaag ttgtcctgtc ccattaagtc
    24361 attggttaag ttatttcgat ttaacatttt tattggaaaa gtttctgagt tgctcttaat
    24421 aaaaaatttt tcttctagct ctctgttttt gcaaaagcca gaaattgttt tgtcaaagat
    24481 accttttttt gatttatagg cttttttatc ttctaccaaa aaataaagtt cttgtggatt
    24541 gtgaataggg gattggattc gaatggataa atctgtttct aaaatatttg tataggcagc
    24601 ttctatacag attggtttat catagagagt gcctcgtaga attggtctta ttactggatc
    24661 agagagaaga gaataggtgt agttattttt ttctgcaaat cttttccagc tttcccatct
    24721 gtcttcttct ttagaatgaa acagaattag aaataaatta tttcgaatct tcatggaaat
    24781 atatttccat ggtttataga tttatcaata attttttgac taaattataa gaatttctta
    24841 aaaaatttca aaagtataaa gtttatccac ttaccatcag gtatttcttc ggtttttcta
    24901 gtgataaatc ctacatgccc cccagtttct gtcaggattg cagttaggtt tttatttttt
    24961 ttccaaagaa cactttgaaa tgaatcgggt ggaattaatg ggtcatcttt tgcatgaatg
    25021 ataataccct tatgttttat ttttggaatg aaatgaatgc tcgagctaga tttataataa
    25081 tcgagtgcac ctttaaaacc aaaaagcgga gccgttacca tatcatcaaa atcaaacata
    25141 gtctttgctt ttaaagcatg ttctttaata tcaggagaag caaataaaaa gccttttttg
    25201 aatttttctt taaaggaatc taaaaagatt tttctataaa agattccggc tggagagtca
    25261 atgtgttcac aggctctttt taaatctaga ggaggggata cagctgaaaa aactttggag
    25321 cgaatcactc ttttttctcc aaagaacttt aaaaccatat tggcagataa agaaaaacca
    25381 cacatgataa ttttttttga aaagtgagtg tatacatatt gttcaatctg accaatatcg
    25441 tctgtttttc ctgcattata gcctttttta gataaaccct cgccgcgacc acatcctctt
    25501 aggttaacac ggattacccc atatccctgt aatagcgcag cctccgctaa ggaaacaata
    25561 taatggctat ctgaactacc ttccatcccg tgaaaaagaa tcatataatg accgttatac
    25621 gggacagcct ttttatccat tttttgcaaa ggtggattgt gatctaacca tagaaaatca
    25681 ccactctcat cttttgtttt taaaattaaa acatctgatt catattttgt tttaaggtga
    25741 ttctttgggg gaaagattgt tgtaaaaata gtctgaacat gactattatt taaaaatttg
    25801 ggagggtcaa gattaattat ttcagtattt ccatccattc ttctacttct tctttgttaa
    25861 agtattttct ttcttttaag aatttcaaaa aatttctaag cataatctta gacttggctt
    25921 gtagttctaa aaagttctca aaattatctt ctagatagaa atttaaaaaa tcatctactt
    25981 cataatgtgt aaaatcttct aggagtattg gttctgtatt ttcaccctct tcatcgacga
    26041 aaagatgaga aagataattg aggataagca caattatctc cccatcgtct ctcttggttg
    26101 catccaagta gagtctgaaa atactttcta aatctttttt atccgacatg aatcgaagtc
    26161 gctagagcgt ctactattcc gtattcgatt gcttctttcg ctgtcatgta ataatctcta
    26221 tctgtatcga gtgcaatttt ttctagaggt tgaccacaag cttcagctaa gattttattt
    26281 aattgttctt ttgttttttt aatttcattt gcatgaattt tgatatcagt tgcaggagct
    26341 acgatttgtc ctccaatact tggttggtga atcattacct ttccacttgg ccaaataaat
    26401 cttcttcctt ttgttcctgc acaaagtaga agggaaccca tagatgcggc cattcccata
    26461 caaacagttg caacaggtga agaaatcatt ttcattgtat cgtatattgt cattcccgca
    26521 gtcacagatc cccctggact attgatataa aaagtaattt cttttcctgg gtctatcatt
    26581 tctagataca tgagtttatt cactatttct ttcgcagagt catccattac tggtccccag
    26641 agaaagatct ttctagtgtc taggaatttc ttctgaataa aatttccatt tttaagttct
    26701 tctaaaacat tctgcgattt ttcatcgcct tctttttcgt cgtctgattt tagtaaattt
    26761 gttggattta tgttcataac taccactttt tattatttaa atttcatgaa aacaccaatt
    26821 aaaatagaac cggcccctac aatataaaaa aacttgagca tattgagagg aggtagatct
    26881 tccttggttt ctttcttttt tacgtttgca gcaggaagcc ctgaagcaaa gaggcgatca
    26941 cttgattcat ttgtaactgc ctctttaaat tttaatacct tagagtcttc ggaaagaaga
    27001 taataacgcc tagcctcacg agcgagagca ttttcatctt ctctcaaaat tttccttttt
    27061 gcttctagat tcatattttc tgtttctaat cgttctacct cttctcgaag aatatttaag
    27121 cgtttctcga gagttctcct ttcaataatc ccatttgccc caagcaggac agaatagatg
    27181 atacaaaaaa tagagagtaa accgattgaa atggaaaatt gattcttact taacatgtag
    27241 gttgtaaaat gttttctttc ctttgtagat tgcacttgtt cctaaatctt cttctattct
    27301 gagcaattcg ttatattttg cgattcgatc tgttctagaa agagatccag ttttaatttg
    27361 tcctgaattt gtagcaacag cgatatgcga aatagtagtg tcttccgtct ctccacttct
    27421 atggcttaca acggctgtgt atccagctct tttcgccatt tcaattgcag ccaacgtttc
    27481 tgtcaaagag ccgatttggt ttactttgat tagaattgag ttacaaattc cttctgaaat
    27541 tcctttttgt aatttctgaa tattggtaac aaataaatca tcccctacga gttggatttt
    27601 gcttcctagt ttttctgtaa gtgatttcca tccagcccag tcattttgat cgagtccatc
    27661 ttcaattgta atgataggat actttgcaac taactctgca tagtagttta caagctcttc
    27721 acttgtcttc tctggatttt tctctgcttt taaaacatac ttctttttct ttttgtcata
    27781 gaattcacta gacgcggcat caagaccgat caatacatcg tcctttggtt tgaatccagc
    27841 tttagtgata gcttctagga ttacttcaaa accttctgca ttgcttttta ggtctggtgc
    27901 aaatccacct tcatccccaa cagcggtatt t
//
LOCUS       DAOQOS010000073        27700 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_786_length_27700_cov_5.462471, whole genome shotgun sequence.
ACCESSION   DAOQOS010000073 DAOQOS010000000
VERSION     DAOQOS010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 27700)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27700)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27700
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_786_length_27700_cov_5.462471"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..208)
                     /locus_tag="PLF66_15700"
     CDS             complement(<1..208)
                     /locus_tag="PLF66_15700"
                     /inference="COORDINATES: protein motif:HMM:NF013205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HMZ65539.1"
                     /translation="MSKFIDEVIIQLKAGDGGPGAISFRHEKFVEFGGPDGGDGGKGG
                     DLYLISDSAVQTLDKYIPLKVYKAS"
     gene            complement(229..861)
                     /locus_tag="PLF66_15705"
     CDS             complement(229..861)
                     /locus_tag="PLF66_15705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008595750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65540.1"
                     /translation="MSKDLFTDLTEDEVAYYYTLLNEEVTNYDCGSLCKGDNNGVPFC
                     CITENAVPLLYKKEFALLKSRSDLWSVWNPTDPKERKLIDEHDDENTVFCKCKGVQFC
                     ERDNRSISCRTFPLEPYIDKRGVFVGLVFMKEFTTGCPLTQRSFDIRQEFIDNHFIFW
                     EKLMLRRPSEFETYTKSSKSYRLSRSKTKKEFPILFPSHLKGKDYLKKYL"
     gene            complement(1159..2583)
                     /gene="hslU"
                     /locus_tag="PLF66_15710"
     CDS             complement(1159..2583)
                     /gene="hslU"
                     /locus_tag="PLF66_15710"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016544457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HMZ65541.1"
                     /translation="MSEIIKPVINYEGETFEMSPRQIVESLDEHIIGQKNAKKAVAIA
                     LRNRMRRRKLEPSMREEIYPKNIIMIGPTGVGKTEIARRLSKLYGAPFIKVEATKYTE
                     VGYVGRDVESMVRDLANIALNMVKSELRLKLVDFARINAEEVILDILIPDNTKPAMGF
                     MSDTNPDDPGEVERRERYLETREMMRKKLKSGKLNEQEIEIDISQSPNQGMPMMQVFG
                     ATNLEEMDNQIQNLLGDLVQKKNKKRKMPIKEALDVLADIEADKLLDQEKLQKEAARR
                     VEEMGIIFIDEIDKIAGKESRGGGADVSREGVQRDLLPIVEGATVNTKIGPIKTDHIL
                     FIAAGAFHMSKPSDLIPELQGRFPIRVELDKLSMEDFVKILTAPKSSLTKQYQALLST
                     EGISIDFKEDAIREIAQIAFDVNEKNENIGARRLNTILERLLEDLSFEGPELVDKNPV
                     IDANFVRNKLNEIVENRDLSKYIL"
     gene            complement(2580..3131)
                     /gene="hslV"
                     /locus_tag="PLF66_15715"
     CDS             complement(2580..3131)
                     /gene="hslV"
                     /locus_tag="PLF66_15715"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008590152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HMZ65542.1"
                     /translation="MKNSFYPDFHSTTILCVRRDGKVALAGDGQVSMGQTVMKHTAKK
                     VRRMFHDKIISGFAGSAADAFTLFELFEKKVNAFNGSLSRSVVELAREWRTDRMLRKL
                     EALLIVADKDESYLVSGTGDVISPDEGILAIGSGGNYAYAAARALYSNTSMSAKDIVL
                     EAMKITADICIYTNHNITLEEIV"
     gene            3348..4913
                     /locus_tag="PLF66_15720"
     CDS             3348..4913
                     /locus_tag="PLF66_15720"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HMZ65543.1"
                     /translation="MNSSISEMPIVRGEIIVNRIRLALFLLYATATVISLGVSHPVLT
                     VAYCIGTISILFYTLYSRAKIQRDEFDIRIVFINSFLDIFIVFILRIMQAIYLENGGD
                     YVLKEKILFSAFYLYIGMIPLRYNEKFALFTGSLILFQEIIIQIIATSLGIEFKTKYG
                     GFNLNEYPLPMIININLFLIASISISYILTKLSQSAIYSANEKENIARESLKKNSQIV
                     ENLKASSIKLVELKEKAKFILGDIQASIMTQASNSEETSSSMEEISAASRSISVATED
                     QNNLSHKTMIIVNENETFFSNLKNAINSLGSLNEKLSNVIKSGKIVIGKTGNSMENMR
                     ESSVGIAKVVNVMREIAYQTNLLALNAAIEAARAGESGKGFSVVADEVGKLASKSTLH
                     TKEITENVQKSLEDINVGSKSVEDVAKAFDQILNSYSEIEKFILVCNDALRNFDKNRA
                     AMINSLDELKNNAMNIRNATNEQEMAVSETTNAMMKISEQANAQADTILELNELVDFL
                     TETEQLINKLTIN"
     gene            complement(4910..5602)
                     /locus_tag="PLF66_15725"
     CDS             complement(4910..5602)
                     /locus_tag="PLF66_15725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002975093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HMZ65544.1"
                     /translation="MVSIKQKIDRKETEKSKRTKELIYSTAISLFQKQSYEKATMRAI
                     ASKAGVALGATYYHFKTKEDIVLQFYTISQDDARLQSLEFCRTTKDFKLRLNNIIRYK
                     LDYFSHYQNFITVLARNAGDPRNPLSPFSKETREIRQEAIQIIRDALETSNLNLKEEI
                     SASLPDLLWLYQLAIIYYWISDSSTNKKNTETLIEESLDLVFKLIKMSKLPFFKTILG
                     SIFKIIKTVKGS"
     gene            complement(5663..6526)
                     /locus_tag="PLF66_15730"
     CDS             complement(5663..6526)
                     /locus_tag="PLF66_15730"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HMZ65545.1"
                     /translation="MKRFTIVLLAFLVQSVFTQEMEKTRDYCGVEDLKCIEKRIKKNE
                     FNYEDLNRQDGNGRTSLHYAVEAKNLELIKVILSNYSNLATISDFNGNLPIHRAAQVN
                     SREILDVLLSYSQSELNRVDRNGETVLDIAQGNGFYELTSYLKSRGGKVGKTHSSSTT
                     LWLYGVYVLISCIITIWVAQTFFKNGRIFLLDAFHSTELADSVNHLLVVGFYLINLGY
                     ITIALKIGSKPEDNIQAVEILSFKLGLVILILGFMHFFNLYLFGRLRKRTLLKKELGL
                     DKNVGVVRPVS"
     gene            6728..7291
                     /locus_tag="PLF66_15735"
     CDS             6728..7291
                     /locus_tag="PLF66_15735"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HMZ65546.1"
                     /translation="MIQLENRNPYSKSEILSSILDIESKVSIFFQSISPELFYHNGLG
                     GWSPAQNLSHLIDIGNGTILLLNTPRFLFSIFGKRKTQRDFETLLTDYIESKAVIQIG
                     PIAPSKISSIDESKKLSVLIPNFKKVYDELNTIIFKIPESEFDEYSLPHPTMGMLSLR
                     EVLYLILIHPIHHCYKVEQKILKVTTE"
     gene            complement(7329..9977)
                     /locus_tag="PLF66_15740"
     CDS             complement(7329..9977)
                     /locus_tag="PLF66_15740"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HMZ65547.1"
                     /translation="MFFRSLKKISFLFYLINLLFLFNVFANETTKENDPKSIQGILDL
                     RNQKFYTTEADDGFIKLDGEWEFYWNEFIDPNLLRQPNNYIQVPSNWNEQTIKYFGEN
                     IHPPANGYASYRIKLLLPEQTPDLAISVEGIDSAYSFFINGVLYSSKGKVGRTIDEME
                     ALNQSSYFSIPKLSEMDIVIHVSNFHHRKAGIRDAIFIGNYSVIEGIQKRNHDFDLIL
                     SSCLLIMGLYHVGLYITRRKDRSPLHFSIFCILIAVRTVSINDRMILGALPLLNFTVV
                     HKLEFVSFYYSSWAFMQFLHYLFPDEFSKKWYWFFASVLVPSSLLVIVTHSSIYTGSL
                     IFVQITILLAIFYSLKVIIAAIVHNRVGAKFFFFGMILFFVSIINDILKSMDIVHTPY
                     MTSYGFISFVLFQAMVLSGKFANAFLESERLALELKAFSESLELKVEERTRSLEEARK
                     QAEIEKNLAFLAQKETQKQKEETEELNLLIKSLNEELDIKLIMKKVKNYIFENYNINH
                     YALYIVNSSRSTISLLDADMPDRISKEDQDKIRSLEIPIGNGEGAHAYVLQTQKPLYI
                     PKVLRTGVSKEELSIIETANIQSFFMIPLFLQSEPIGIIDFSSDERMKLSENDVTRLS
                     ILGEQLAGVIYGSNLYKQVQEEKEKSDRLLLNILPKEIAVELKEKGFSEPISFESVSV
                     LFTDFKGFTQIAEKLPPSELVKELDACFVQFDKITERYNLEKLKTIGDSYMCAGGIPT
                     KNRTHAIDCVLAAIEIQSFMEMMKDLKTTKGFSYWELRLGIHSGPIVAGVIGEKKFAY
                     DVWGDTVNTASRMESSGTPGKINVSGSTYQIIRKYFECEYRGMVKAKNKGEVEMYYVL
                     GLKKEFSIRGDGKTPNEEFWKEYRKI"
     gene            complement(10153..10923)
                     /locus_tag="PLF66_15745"
     CDS             complement(10153..10923)
                     /locus_tag="PLF66_15745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004424264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HMZ65548.1"
                     /translation="MEVKGKTVLITGSASGLGKSMALGFGKLGANVIVSDIKQETIDL
                     VVNEIKSNGGNAFGIPGDVSNEADAVRMMEESVSKTGSLDVAILNAGILRDGLLVKVD
                     KETGNVKGKMSIEQWQAVINVNLTGVFLTGREAAVQMINSKKGGVIIPISSVAMHGNP
                     GQSNYSAAKAGVAAMTKLWAHELKNFKIRVAGIAPGFIATEMVLKDMNPAALEKWKSL
                     IPIGRLGEPDEIFKTAQFIVENDLIDGVVLEASGGIKI"
     gene            complement(10933..11616)
                     /locus_tag="PLF66_15750"
     CDS             complement(10933..11616)
                     /locus_tag="PLF66_15750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001129801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HMZ65549.1"
                     /translation="MEKTPENLPDKVRAMVAAREKISLKSSKINARLEKYVLLIITEI
                     LIKLGQERYTEMIYIIVKELAINAVKANQKRIFFEDLGLNILDENQYAEGIIKFKQSF
                     SDSMAEVYGAKCQEKGVYVQINFFYKTAGMYVEVTNNTPVIKAEELRMREKMKKAMSY
                     NDIAEFYMDNMDNTEGAGLGIALIMILMKNESLDPNLFRIVTKPTETIARIEIPFNSD
                     YVSFRNKGA"
     gene            complement(11679..12002)
                     /locus_tag="PLF66_15755"
     CDS             complement(11679..12002)
                     /locus_tag="PLF66_15755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65550.1"
                     /translation="MNSSFFNWLVFSVVMVANSIGVFSVFKPIRNPILHSLHHIFYFL
                     VIVSTLFSIGFSFYIQSLSYSHLGLLFLMTLLPFFRKGGREHRLIGVLSFFCAIIPLI
                     LKYFN"
     gene            12068..13336
                     /locus_tag="PLF66_15760"
     CDS             12068..13336
                     /locus_tag="PLF66_15760"
                     /inference="COORDINATES: protein motif:HMM:NF024806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HMZ65551.1"
                     /translation="MKKIFLFLFITLSLYPVSREDKIVYAFRDTGSGNYSKMVIIGET
                     VSIDKASMMDGNNKIDRDLMQIGVDLRPDTLTVKVLYNPGIRVGQTLYLIEKNPDHIS
                     YKDGNIVGQVKVVSIFNTSFFGQQIRGEGYLRLIDNKVMTVAMPIESESLEDAILLKK
                     QADYFIAKGDIASAITYYKKAIKMDKNYPEAHYFLGKIHSSGKYGVSSESDEQGSGEG
                     YVSAGYEYKIAWNNRDKFLDKHEKFQFYLDYMKYLINRFEIESFKNTNLTTDLDTCLE
                     ISKEALKMSPSNFDALHSISYSYFLYYLRTRDTQVTVDSEEKMAAERINIRKKQSEYL
                     EKAQDTLEKALKIRHQDHKIQALAILLYFEKLRELPNSESLSQVTERNQLRTKIEAHG
                     KQYLIYKPKSDKKDKLEKRIKDILYTAKGF"
     gene            13470..15407
                     /locus_tag="PLF66_15765"
     CDS             13470..15407
                     /locus_tag="PLF66_15765"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HMZ65552.1"
                     /translation="MSEIDSTASICILCNEERTPDGITTNGVFNCITCGNEWILEKRK
                     SQRLAKIQFTGDYSNLITEFVTIFDSNLNLRQLCDKTLSIIKTKFGIEKLGFMIHEPV
                     NQFFKFEKYVGSENASKVITKLKTNSEDTNHPFAKSILSKKPSIRFLNASNRFDKIYT
                     RVTEGVVSQILVPIVYKSMPLGLLVFDFDDKRIAEEKFLDKDVFNVISNQVAIALYHT
                     IIFNKSNTKYKQFVNLHSSGLTLNKHYLNNTLEIIKMSLLTISGLVETDVNILLIHNK
                     KFKVITYHKLIRTIDSLDLSQDSILMDNNEPYKAFFNKQTPTLLETENEPLSKKLGFT
                     GKQILNLPSFTLEVNEYVFLLGRNSTILFTPDEVEVLAAYSELVKITIDNAFLYHKMA
                     KQERLEKEVEIAREIQLNLLPREMPKHPRYEFAGFMVPAREIGGDYYDILVSPDQSET
                     LFAIGDVSGKGLPAGMVMVTARTIIHSVIRRISELKDIVMELNSYLYYNSKNAATMRF
                     MSLTCLKWNVESNVFQYSGCGHGNLLVYRSDKNTVEVIFTGGTVLGITDDISPMYSEG
                     TIELGIGDSILMYTDGVTETMNTRGEQLEEEGLIRSFKKYNKEKPAEILEGIYKDLKD
                     YSVNVTSQHDDITMLIIKRFK"
     gene            15404..15712
                     /locus_tag="PLF66_15770"
     CDS             15404..15712
                     /locus_tag="PLF66_15770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016546714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HMZ65553.1"
                     /translation="MIFQDSTYKIFLEIFNRLGSYQKLIQNYKAIIGFSGGKDSTLLA
                     YFYKFLNQEKLCPEPILFHLDHSIRNNFEQEEEIELYMKDFFSNSIYKKKTFQKSRSI
                     "
     gene            16498..16665
                     /locus_tag="PLF66_15775"
     CDS             16498..16665
                     /locus_tag="PLF66_15775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65554.1"
                     /translation="MRKTVSEILRMYEIPRRVRFKIPMLFRNQQLCSILFSLWDDKLK
                     DFQGDLYYEAK"
     gene            16652..17248
                     /gene="yihA"
                     /locus_tag="PLF66_15780"
     CDS             16652..17248
                     /gene="yihA"
                     /locus_tag="PLF66_15780"
                     /inference="COORDINATES: protein motif:HMM:TIGR03598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HMZ65555.1"
                     /translation="MKQNKLVIHSSAFHSSHSDWKKIPENPTMPQIAFCGRSNAGKSS
                     LLNAILARKEIARVSATPGKTRLINFFLLNDHYFLVDLPGFGYAKASNQTRDDLINIV
                     NGYLNHVKTLKLLFVLCDASRPLPDEELSLIDVASAKKIKVALVRTKVDKLNQKEKAK
                     LMKELEEIKKEYTELDIFLSSTKTNEGILELRNLLAEY"
     gene            complement(17650..17961)
                     /locus_tag="PLF66_15785"
                     /pseudo
     CDS             complement(17650..17961)
                     /locus_tag="PLF66_15785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008590989.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(18123..18596)
                     /locus_tag="PLF66_15790"
     CDS             complement(18123..18596)
                     /locus_tag="PLF66_15790"
                     /inference="COORDINATES: protein motif:HMM:NF033171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipoprotein"
                     /protein_id="HMZ65556.1"
                     /translation="MKVIFPILISVLFLLINCQVSESVGSASTSLNGVSTSSNSISKI
                     SDSVQSLSGSIQSISGSSSGGSKAQMQTYKMDVRDLTAIYYQTGFNHEFKKDLNTVAQ
                     KNGLLEWQTDALTYIAIGEGLKKANMSEYDFKVFLSNFESKKELGKFLKEGYTSL"
     gene            complement(18655..20565)
                     /locus_tag="PLF66_15795"
     CDS             complement(18655..20565)
                     /locus_tag="PLF66_15795"
                     /inference="COORDINATES: protein motif:HMM:NF013239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HMZ65557.1"
                     /translation="MNKATLKYKIFNKIYNSEFRKHSENRYLSEDNLFELKFQRKKDK
                     LGAILFIGSLSWIDHQRPSIGLVILDLRIDLNFDESFTNLNILQHGYQSWSFTTRYSS
                     EDKDYSPRLLGFLKYGQENIYTDHAGKPGNFVSEGLLIGYSKTEEKGFLIGVNEIGSQ
                     FTKFHILWDANKINSLSIIYDFYSTPEFKNNTPVELTQVRLDLFNDTPPELILEEYAS
                     GLGKKAKVKTFEENVPTGWCSWYYYYTNISEEIILSNLHEVKNRKLPIEFFQIDDGYQ
                     NEIGDWTVVNEKFPRGVRFLAEEIKKQGLTPGIWIAPFLVRQKSSFFTLYPEAILKDA
                     KGKPVPAIWNPLWGLDFTYTLDVTHPKSIEFLEELFKTFTKDYGYSYLKLDFLFSASL
                     PGVPYNKRLTPFERYRQALELIRKVVGKNTFLLGCGAPLIPSIGIFDGMRIGCDVTPF
                     WYPEIKRRMLRDKHALSTEKGLINTINRSFMHRNLWLNDPDCLIVRKEKNKMTPEQTI
                     LMATVMAMSGGILLVSDNLVTIEEDRVQILQKALDLSKLCQKKKSIPLGMFGNSFPRG
                     FINENGFFAVWNPTPKDDWIELDIPFKVKLIDSNDYWTGEKIYSLKVDNNLKKIKVML
                     KPFQAIVIGVKN"
     gene            20744..21382
                     /locus_tag="PLF66_15800"
     CDS             20744..21382
                     /locus_tag="PLF66_15800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017214518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HMZ65558.1"
                     /translation="MQQKHSPLKPKVFQSDIDEEYFEAYSKDTHLAIDCEMMGLNPRR
                     DRLCLIQICDSSNRFSLIQILPDQKQAPLLKKLFENPDIVKIFHFARMDMLFLKTYLG
                     IQVNKVFCTKIASKLARTYTDKHGLKELIREFFDVNIDKKNQSSDWGKKVLTKDQLEY
                     ATEDVRYLISLEHQLTEILIRENRYEIASKCFQFLPTLIDLDFLELKDVLEH"
     gene            21379..22740
                     /gene="radA"
                     /locus_tag="PLF66_15805"
     CDS             21379..22740
                     /gene="radA"
                     /locus_tag="PLF66_15805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020771191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HMZ65559.1"
                     /translation="MKKQVRLFICQSCGQEYSRWAGKCESCGEWNTIQEEIGQERFSK
                     QSFKSQVDKYSKPKPISEINEEAYKRIPTGFKELDHVLGGGIVPGSMTLIGGEPGVGK
                     STLILEISKNISKLGKVLYISGEESAGQISLRAKRMGIESKNIFISSEIYAENIASMI
                     ANEEPTIVFVDSIQTISKESIPNAAGTVTQLRECSQLLLETAKKTNIPIFMIGHITKE
                     GSIAGPKVLEHIVDTVLYFESDKLNYYRLLRGIKNRFGAVGDIAVFEMRSNGLTEIID
                     KHNLFISPDHENRTGSILSVVMEGSRSLSVEVQALVSRSSYSQARRTAEGLDNKRLVL
                     LAAVLEKFMGIKTIECDIFCNLAGGLNIDEPALDLAICASIVSSYIEKPAKKNYAVIG
                     EVGLSGEVRTVSHIHTRLKDLTSIGITNIILPKGNLTEVKDKNHKIELIPIESIRELE
                     KLF"
     gene            complement(22800..23084)
                     /locus_tag="PLF66_15810"
     CDS             complement(22800..23084)
                     /locus_tag="PLF66_15810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011669658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65560.1"
                     /translation="MNEITIFAMALLAVPVFARFARVGKETMARFHLIALGGIFLLLG
                     EATRLTAAKIAIIATILPIVDFTSALAAYSLVLLGTIWLSLYYIKHPSEI"
     gene            23314..24006
                     /locus_tag="PLF66_15815"
     CDS             23314..24006
                     /locus_tag="PLF66_15815"
                     /inference="COORDINATES: protein motif:HMM:NF017672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0006672 - ceramide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ceramidase domain-containing protein"
                     /protein_id="HMZ65561.1"
                     /translation="MEIIQLPPECFWSSFAPPTIKYCEENICSIITQPSNTWSNLAYI
                     FIGFYLIWKNKNDSISSLKLVGPMAIATGIASFLYHASFTFFFQFFDLSSMYLFSTMI
                     LSMNLKRAKIIKKEYQHGTQFIILAASMLLLYIFKPFGIPIFALQVLAGLILEFYIFR
                     KMKEEITYSYYKLSLLFLVLAFVAWTVDFRRIICDPMNHILQGHAVWHILSSSCFLFL
                     YKFYSQEKLEKT"
     gene            complement(24054..25040)
                     /locus_tag="PLF66_15820"
                     /pseudo
     CDS             complement(24054..25040)
                     /locus_tag="PLF66_15820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015677674.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
     gene            complement(25046..25825)
                     /locus_tag="PLF66_15825"
     CDS             complement(25046..25825)
                     /locus_tag="PLF66_15825"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004786974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acyltransferase"
                     /protein_id="HMZ65562.1"
                     /translation="MAISVISKKQGVERKLEVRLAENQYEIEQTLSLRYNVFNLEMGE
                     GLPESRQAQKDRDEYDYYSDHLIVKDETANKIVGTYRILKRSVAKANIGFYSENEFDL
                     SKIYEFKDEVAEVGRSCVHADYRDGSVISLLWSGFGEYMLKNDIRYLMGCGSIHSMDS
                     IVASQAYAYLKENNALAGEEFGVTPYESYQLVGFDPNYKVADWKAVNKAIPPIIKGYV
                     RLGAKICGYPALDRIFKTTDVFVLFDRKEIDERYGKHYLKA"
     repeat_region   26002..27636
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=26074..26109
                     /rpt_unit_seq="gtgctcaacgcctaatggcatcaaagataatttcat"
BASE COUNT         9332 a         4940 c         4240 g         9188 t
ORIGIN      
        1 aacttgcttt atacactttc aaaggaatat atttatcgag agtttgaaca gcggagtctg
       61 aaataaggta tagatctcct ccctttcctc catcgccacc atccggtcca ccaaattcta
      121 cgaatttttc atgtcgaaag gaaatcgcac ccggtccccc atctcccgct tttaattgta
      181 taatgacttc atctataaat tttgacatag tttactcgta acagtatttt aaagatattt
      241 ttttaaataa tctttccctt tcagatgaga tggaaataga attggaaatt ctttttttgt
      301 tttagaacgc gataggcgat aagactttga tgattttgtg tatgtttcaa attcagaggg
      361 tcttctaagc attaactttt cccagaaaat gaaatgattg tcgataaatt cttgtcttat
      421 atcaaaactt ctctgagtaa gtggacagcc cgtagtaaac tctttcataa aaacaagtcc
      481 tacaaagact cctcttttat caatataagg ttcaagtgga aatgttctac aactaatgga
      541 acgattgtct ctttcgcaaa actgaactcc tttgcattta caaaagacag tgttctcatc
      601 atcatgttca tctattaatt ttctttcctt tggatctgta ggattccaaa ctgaccataa
      661 atcagatcta gattttaata gggcaaattc ttttttgtat agaagtggaa ctgcattttc
      721 agtaatacag cagaaaggga caccgttatt atcccctttg catagactac cacagtcata
      781 gtttgttact tcttcgttta agagtgtata gtaataagca acttcatctt ccgtaagatc
      841 agtaaataaa tccttactca tacatacttt aaaactgtta agcccttcat tgacaagaat
      901 aaatccattt ttataaatac tactcccttt atggcaaagt tagatttgta gatttaattg
      961 cgatttttct atgtaggggt tacctagaac tccaaagctt aatcgaactt cttttcttac
     1021 gtatcctttc actcacactg tttttgtaat gcgaataaaa ggaatgttca ttgaaatctt
     1081 ctaagtaagc tttgaatgca acaatttcga aattcaatag gactttttaa tttttaatca
     1141 tccaatgatg aaagtaaatt atagaatata cttcgagaga tctctgtttt caacgatttc
     1201 atttaattta tttctaacaa aatttgcatc aatcacagga ttcttgtcta ccagttccgg
     1261 accttcaaaa cttaaatctt ctaaaagtct ttctagaatt gtattcaatc ttcttgcacc
     1321 aatattctca tttttttcat ttacatcaaa tgcaatttga gcaatttctc ttatcgcatc
     1381 ctctttaaaa tcaatcgaaa taccttccgt tgaaagtaga gcctgatatt gtttagtgag
     1441 agatgatttt ggtgctgtaa gaatttttac aaagtcttcc atagaaagtt tgtctaattc
     1501 gactcgaatt ggaaaacgac cttgtagctc tggaattaaa tccgaaggct ttgacatatg
     1561 aaatgcgcca gcagcaataa ataaaatgtg atctgtttta ataggaccaa tttttgtatt
     1621 tacagttgcc ccttctacta tcggtagtaa atctctctgc acaccttctc ttgatacatc
     1681 agcccctcca cctctacttt ccttcccagc gattttatct atctcatcga taaatataat
     1741 ccccatctct tctactcttc ttgctgcttc tttttgtaat ttttcctgat cgagcaattt
     1801 atccgcttca atatcagcta agacatctaa cgcttctttg attggcatct ttcttttttt
     1861 attttttttc tgaactaaat cgcccagtaa attctgaatt tgattgtcca tttcttctaa
     1921 attcgttgca ccaaaaactt gcatcatcgg cattccttgg tttggactct gggaaatatc
     1981 aatttcaatc tcttgttcat ttaacttccc tgactttaat ttctttcgca tcatttcccg
     2041 agtttctagg tatctttccc ttctttccac ttctcctgga tcatcagggt ttgtgtcgga
     2101 cataaaaccc atagcaggtt ttgtattatc tggaattaaa atatccaaaa tcacttcttc
     2161 cgcattgatt ctggcaaaat cgactagttt taatctcaat tcggacttaa ccatattcaa
     2221 ggcaatatta gctagatctc taaccatcga ttcaacatcg cgcccaacgt atccaacttc
     2281 tgtatattta gtagcttcta ctttaataaa gggagcacca tagagtttag aaaggcgtcg
     2341 agcaatttca gtttttccca cacctgtagg accaatcatg ataatatttt ttggataaat
     2401 ttcttcccgc atcgaaggtt ctaatttgcg tctacgcata cgatttcgaa gcgcaatggc
     2461 tactgccttt tttgcatttt tttgcccaat aatatgttcg tctaaacttt caacgatttg
     2521 tcttggcgac atttcaaatg tttcgccctc ataatttata acaggtttaa ttatttcact
     2581 catacgattt cttctaatgt tatattgtgg tttgtataaa tacaaatatc tgctgtaatt
     2641 ttcatagctt ccaatacaat gtcttttgca ctcattgagg tatttgaata aagagctcta
     2701 gctgcggcat acgcatagtt gcctcctgag cctattgcaa gaatcccctc gtctggagaa
     2761 ataacatctc ctgttccaga caccagataa gattcatcct tatctgcaac aatgagaaga
     2821 gcttctaact ttcggagcat acgatctgtc cgccattcac gagctaactc aacgacactc
     2881 ctagaaagac taccgttgaa agcatttact tttttttcaa aaagctcaaa gagagtaaat
     2941 gcatctgccg ctgagcctgc aaaaccagaa ataattttat cgtgaaacat tctgcgaact
     3001 tttttcgctg tatgcttcat tacagtttgt cccatcgaaa cttgtccatc tcctgctaga
     3061 gcgacttttc catccctacg cacacataga atcgtagttg aatggaaatc tggataaaaa
     3121 gaatttttca tagttatttt tctcatgtat aagatctgaa tcagtaacca tcttagagac
     3181 tttcaatcac cttctaaaaa aatggaaatg acttaaggat aagatttcaa aagggctttt
     3241 tttatctaca attatatacc aagcacagtg aaagcaaatt atctggtaaa aatagcaaat
     3301 gatcagattc atttgaaaat taagatgaat catccgacac tatatatatg aattcctcta
     3361 tttcagaaat gcctattgtg cgcggagaaa tcattgtaaa tagaatccgc cttgcactct
     3421 ttcttttgta tgctactgcg actgttatat cactcggtgt atcgcatcca gtccttacag
     3481 ttgcgtattg tattggaaca ataagtatat tgttttatac actctactca agggcaaaaa
     3541 ttcaaagaga tgaatttgat attcgtattg tttttataaa ttcttttcta gatatcttta
     3601 ttgttttcat acttcgtatc atgcaagcaa tctatcttga aaatggcgga gattatgttt
     3661 tgaaagagaa aattctattt tctgcttttt atctttatat tggaatgata cctcttcgct
     3721 ataatgaaaa atttgctttg ttcactggta gccttatttt atttcaagaa attataattc
     3781 aaataatcgc tacaagcttg ggtattgaat tcaaaacaaa atacggagga tttaatttaa
     3841 acgaatatcc tcttcctatg attattaata taaatctatt tctaatagct tccatttcta
     3901 tcagctatat tttaacaaag ctgagccaat cagcaattta ttccgcaaat gagaaagaaa
     3961 atattgcaag agagtccttg aaaaaaaact ctcagatagt agaaaaccta aaagcatcta
     4021 gtataaaatt agttgaattg aaagaaaaag caaagtttat attaggagac attcaagctt
     4081 ctattatgac acaagcatct aattcagaag aaacctcatc ctcgatggag gaaatttccg
     4141 cggcatctag aagtattagt gtggcaacag aagaccaaaa taatctatct cataaaacca
     4201 tgattatagt aaatgaaaat gaaacatttt tttcgaattt aaaaaatgca attaactcgc
     4261 taggctctct gaatgaaaaa ttaagtaatg taattaaaag tggaaaaatt gtaatcggaa
     4321 agactggaaa ttccatggaa aatatgcgag aatcttctgt tgggatcgca aaagttgtaa
     4381 acgttatgcg agaaatcgct tatcaaacca acctactggc attgaatgca gctatagagg
     4441 cagctagagc cggtgaaagt ggaaaaggat tctctgtggt cgcagatgag gtcggaaaat
     4501 tagcaagcaa gtctactctg catacaaagg aaattacaga aaatgtccaa aagtcattag
     4561 aggatataaa tgtaggaagt aaatctgtag aagatgtggc aaaagcgttc gaccaaatac
     4621 taaatagtta ctcagaaatt gagaagttta ttttagtttg taatgatgcg cttagaaatt
     4681 ttgataaaaa tagagctgca atgatcaatt ctctagacga attaaaaaat aatgctatga
     4741 atattcgaaa tgctacgaat gaacaagaaa tggcagtgag tgaaacgact aacgctatga
     4801 tgaaaatttc ggagcaggca aatgcccaag cagatacaat actggaatta aatgaactcg
     4861 tagatttctt aacagaaaca gagcaattga taaacaaact tactataaat taagatcctt
     4921 ttactgtttt tattatttta aaaatagacc ctagaatcgt cttaaaaaaa ggaagtttag
     4981 acatcttaat tagtttaaaa actaaatcta aactttcttc aatcaaggtc tctgtatttt
     5041 ttttatttgt cgatgaatct gatatccaat agtaaataat tgcaagttga taaagccata
     5101 atagatccgg aagacttgcg gaaatttctt cttttaaatt taaattcgat gtttctaatg
     5161 catctcgaat aatttgaata gcttcttgtc gaatctctct agtttcttta ctgaatggag
     5221 aaagtggatt tctaggatca ccagcatttc tagctaaaac agtaataaaa ttttgataat
     5281 gtgaaaagta atccaattta tagcggataa tattatttag gcgtaattta aaatctttcg
     5341 tggttctaca aaactctaga ctttgtaatc tagcatcatc ttgtgaaata gtataaaatt
     5401 gtaatacaat atcttccttg gtcttaaaat gataataagt tgcgcctaat gctacacctg
     5461 ctttacttgc aattgctcgc atggtagctt tttcataact ttgtttttga aaaagagaaa
     5521 tggctgtaga ataaatcaat tccttcgttc gttttgattt ttctgtttct tttcgatcta
     5581 ttttctgttt tattgaaacc ataaatatta tgaagtaaat ggctattagc ctctgccaaa
     5641 agccatttac agattccatt ttttaagaga cgggtcttac tacacctaca tttttatcca
     5701 aacccaattc ttttttcaaa agagttctct ttctaagtct accaaataag tagagattaa
     5761 aaaaatgcat aaaacctaaa atcaatatca ctaaacctaa tttaaaactt aaaatctcta
     5821 ctgcttgtat gttatcttct ggtttagatc caattttcag tgcgatagtt atataaccta
     5881 gattaatgag ataaaaacca acgactaata aatgattcac tgagtccgca agctctgtgc
     5941 tatgaaatgc atctaagagg aaaattcgac catttttaaa aaatgtctgt gcaacccaaa
     6001 tagtgatgat acaactaatt agaacgtaaa ccccatagag ccaaagagtt gtagaagaag
     6061 aatgagtttt tccaactttt cctccgcggg attttagata actagtgagt tcatagaaac
     6121 catttccctg tgctatatct aaaacagttt ccccattcct atccactcgg tttaactctg
     6181 attggctata actaagtaga acatccaaaa tctctcttga attgacctgt gcagctctat
     6241 gaattggcaa atttccatta aaatcggaga ttgtagctaa gttagaataa tttgataaaa
     6301 tcacttttat taactcaaga tttttagctt ccactgcata atgtagactc gttctaccat
     6361 ttccatcttg gcggttcaag tcttcatagt taaactcatt ttttttaatc cttttctcga
     6421 tgcattttaa atcttctacg ccacagtaat ctctggtctt ttccatttct tgggtaaata
     6481 cactttgaac tagaaatgct aacaatacaa ttgtgaatct tttcataaga atctcctttt
     6541 tgaacatgtt caatattatt aaagaacaag aaaaattcaa ttcttttttt gaacatgttc
     6601 atttttttaa ataacaggac tgaaatgggt gtctttcagc gcaaagaatc aatatttttt
     6661 tactctttac ttcaatttat tttttaattg ctttttattg tgtttaaatc caagttaaca
     6721 ataaacaatg atacaattag aaaacagaaa cccttactct aaatcagaga ttctctcctc
     6781 tatcctagat atcgaaagta aagtaagcat tttttttcaa tctattagtc ctgaattatt
     6841 ttatcacaat ggtttaggag gctggtcacc tgcacaaaac ttatctcatc tgattgacat
     6901 tggaaatgga actattctat tactcaatac acctagattt ctattttcta tttttggaaa
     6961 aagaaagacg caaagagatt ttgaaactct attaacagac tatatagaaa gtaaagcagt
     7021 aattcaaatt ggaccaattg caccaagcaa gatttcgtct atcgatgaat ctaagaaact
     7081 aagtgtgtta attcctaatt ttaaaaaagt ttatgatgag ttgaatacta tcatttttaa
     7141 aattccagaa tctgaatttg atgaatattc tcttcctcat cctaccatgg gaatgctcag
     7201 tcttagagaa gtactgtatt tgattctcat ccatccaatc catcattgct ataaagtaga
     7261 acagaaaatt cttaaagtaa ctacggagta aaagatttgg attatgaaaa cattatttca
     7321 tagccaattt aaatcttcct atactctttc caaaattctt cattcggagt tttgccatct
     7381 ccccgaatag agaattcttt ttttaatcca agaacataat acatttctac ttcaccttta
     7441 ttttttgctt ttaccattcc tcggtattca cattcaaaat attttcttat aatttgatag
     7501 gtagacccac tgacgtttat tttacctgga gttccagaag attccattcg acttgctgta
     7561 tttacagtgt ctccccagac gtcataggca aatttctttt cgccaataac tcctgcaaca
     7621 attggaccgg agtgaattcc aagtcttaat tcccaatagg aaaatccttt tgttgttttt
     7681 aggtctttca tcatttccat aaaactttgt atttcaatag ccgcaagaac acaatctatc
     7741 gcgtgagttc tattttttgt tggaattcca ccagcgcaca tataactatc gcctattgtc
     7801 tttaatttct ctaaattata cctctctgta attttatcga attgaacaaa acaggcatcc
     7861 aattctttta ctaattcact tggaggaagt ttttcggcaa tctgagtaaa ccctttaaaa
     7921 tcagtgaata atacagatac agattcaaaa gaaattggtt ctgaaaaacc tttctctttt
     7981 aattcaactg ctatttcttt aggtaatata tttaaaagta aacgatctga tttttctttt
     8041 tcttcctgaa cttgtttgta aagattggac ccataaataa ctccagctaa ttgctcaccg
     8101 agaatcgata ggcgcgttac gtcattctcg gaaagcttca ttctttcatc actggaaaaa
     8161 tcgataatac cgatgggttc actttgcaaa aacaaaggaa tcataaaaaa agattggata
     8221 ttcgctgtct caatgatgga aagctcttcc tttgaaactc cagtgcgtaa aacttttgga
     8281 atataaagag gcttttgtgt ttgtaatacg tatgcatgag cgccttcacc atttccaatt
     8341 ggaatttcta agcttctaat tttatcttgg tcttctttag aaattctatc gggcatatcg
     8401 gcatccaaaa gcgagatagt gcttcgactt gaatttacta tatacaaagc atagtgatta
     8461 atattgtaat tttcaaatat atagttcttc acttttttca ttataagctt aatatccaat
     8521 tcttcattta aactttttat aagaagattt aattcttcgg tttcttcctt ctgcttttgt
     8581 gtttcctttt gtgcaagaaa agccaagttt ttttctatct cggcctgttt tctcgcttct
     8641 tctagactac gcgttctttc ttctactttt agttccaaac tctccgaaaa tgcttttaat
     8701 tccaatgcta gtctttctga ttctaaaaat gcattcgcaa attttccgga cagaaccatc
     8761 gcttgaaaca atacaaaaga aataaatcca taactcgtca tatagggagt atgaacaata
     8821 tccattgatt ttaaaatatc atttataata gatacgaaaa acaatatcat cccaaaaaag
     8881 aaaaattttg ctccaactct attatgaaca atagccgcaa tgattacctt taaggaataa
     8941 aatattgcca atagaatagt aatttggaca aatattaaac taccagtgta gatagagcta
     9001 tgtgtcacaa taacgagcaa tgaagaagga actagaacag atgcaaaaaa ccaataccac
     9061 tttttggaga actcatctgg aaatagataa tgcaagaact gcataaatgc ccaagatgaa
     9121 taataaaaag aaacaaattc taatttatgg acaactgtga aatttaaaag cggtaatgca
     9181 ccaagaatca ttcgatcgtt aatcgaaaca gttctcactg caattaatat acagaatata
     9241 ctgaagtgta gcggagacct atcttttctt cttgtaatat aaagaccaac atgatagagt
     9301 cccattataa gcagacaact cgataatatt aaatcaaaat catggttcct tttttgtatt
     9361 ccctcaatga ctgaataatt cccaatgaat atggcatcac gaattcccgc ttttctgtga
     9421 tgaaaatttg aaacatgaat aacaatatcc atttccgata atttgggaat tgaaaaataa
     9481 ctggattgat tcaatgcttc catttcgtct attgttcggc caactttgcc tttagaggaa
     9541 taaagaaccc cattaataaa aaatgaataa gcagaatcaa ttccctccac tgatattgct
     9601 aagtctgggg tctgttcagg aagtaataat tttatacgat aactggcata tccatttgct
     9661 ggtggatgaa tgttttcacc aaaatattta atagtttgct cattccagtt tgaaggaact
     9721 tggatgtaat tatttggttg tcttagtaga tttggatcaa tgaattcatt ccaataaaat
     9781 tcccattctc catctaattt aataaaacca tcatctgctt ccgttgtgta gaatttttga
     9841 tttcgtaaat ctaaaatacc ttggattgat tttggatcat tctcttttgt tgtttcattt
     9901 gcaaatacat taaataaaaa aagtaaattg attaaataaa acagaaagga aatttttttt
     9961 agcgatctaa aaaacattac accataaatt aatacaaaac gcatacattt caagcgttat
    10021 tttctttata tgaaaaatca tgcttagaat tatttatgaa gtgtataaaa aaaaggcgag
    10081 acgataggca tctcgcttta attttttatt gggatgattt gaactattct cttttggtag
    10141 aatctttgaa ggttaaattt ttatcccgcc agaagcttct aaaacaacac catcaattag
    10201 atcattttct acaatgaatt gggcagtttt aaaaatctca tcaggttcac caagtcgacc
    10261 gattggaatg agtgatttcc acttttctaa tgctgctgga ttcatatctt ttaaaaccat
    10321 ctctgttgcg ataaatccag gcgcaatacc tgccactctg atcttaaaat ttttaagttc
    10381 atgtgcccag agctttgtca ttgccgcaac tcctgctttt gcagcagaat aattactttg
    10441 tcctggattt ccatgcattg cgacagacga tataggaata atgaccccac cctttttaga
    10501 attaatcatt tgaaccgcag cttctcttcc tgttaaaaac acacctgtta agtttacatt
    10561 tataactgcc tgccactgct caatagacat ttttcctttt acattccctg tttctttgtc
    10621 caccttaaca agcaatccat cccttaaaat acctgcattt aatatagcaa catctagaga
    10681 gccagtttta gatacactct cttccatcat acggactgca tccgcttcat ttgaaacgtc
    10741 acccggaata ccaaaggcgt ttccaccatt tgatttaatt tcatttacaa ctaaatcaat
    10801 tgtttcttgt ttaatatcac ttactattac gttagctcct agtttaccaa aaccgagagc
    10861 catagacttt cctagaccac ttgctgaacc tgttataaga acagtttttc cttttacttc
    10921 catgctttta aatcaagctc ctttatttct gaaagataca tagtcagaat taaagggtat
    10981 ctcaattcta gcaatcgttt ctgttggctt cgttacaatt ctaaataggt ttggatccaa
    11041 actctcattt ttcataagaa tcataattaa cgcaatacca agacctgctc cttcagtatt
    11101 atccatatta tccatataaa actcagcaat atcattgtag ctcattgctt ttttcatctt
    11161 ttctctcatt ctgagttctt ctgctttgat aacaggagta ttattagtta cctctacata
    11221 cataccagca gttttataaa aaaaattaat ttggacgtat acaccttttt cctgacactt
    11281 tgctccataa acttcagcca tagagtcaga aaaactctgt ttgaatttta taatgccttc
    11341 cgcatattga ttttcatcca aaatattgag tcctaaatct tcaaaaaaga tcctcttttg
    11401 gttcgctttc acagcgttaa ttgcgagttc tttgacgatt atataaatca tctctgtata
    11461 tctttcctga cctaatttga tcaaaatttc tgtaattata aggagaacat acttctcgag
    11521 gcgagcatta atcttagaag acttaagcga aattttttct cttgcggcaa ccattgctct
    11581 aactttatca gggagatttt ctggagtttt ttccataaca catcctaaaa ttcattctaa
    11641 gaaacgaata tgcaactata gttagcaata tcgaatagtc aattgaaata tttaagtatt
    11701 aaaggaatta ttgcgcaaaa aaaagaaagc acaccaatca aacgatgttc tcttccacct
    11761 tttctgaaaa atggcaacag ggtcatgaga aaaagaagac ctaaatgaga ataacttaaa
    11821 gactgaatat aaaaactaaa tccaatagaa aataaagttg atacaatcac taaaaaataa
    11881 aaaatatggt gcagggaatg gagaattgga ttgcgaattg gcttaaaaac agaaaatact
    11941 cctatactgt ttgctaccat tacaactgag aatacaagcc aattgaaaaa actagaattc
    12001 atataaattt agattctttt ccatttccta aaactaccga tatttaaaag agtatatgat
    12061 gccaacaatg aaaaagatat ttttgttttt atttataaca ctatccttat atccagtcag
    12121 ccgcgaagat aaaattgtat atgccttccg agatactggc agtggaaact actctaaaat
    12181 ggtcatcatc ggagaaacag taagtatcga caaagcttct atgatggatg ggaataataa
    12241 aatcgatcga gatctaatgc aaattggtgt tgatttaaga ccagataccc tgaccgtaaa
    12301 agtcctctac aatcctggaa ttcgagtagg acaaacactc tacttaattg aaaaaaatcc
    12361 tgatcatatt tcttacaaag atggaaatat cgttggtcaa gtaaaagtag tatctatatt
    12421 taatacttca ttctttggtc aacaaatcag aggtgaaggt tatttaagac ttatagacaa
    12481 taaagtaatg acggttgcaa tgccgatcga atctgaaagt ttagaagatg ctattttatt
    12541 aaaaaagcaa gctgactatt ttattgcaaa aggtgatatt gccagtgcaa tcacctacta
    12601 taaaaaagcg attaaaatgg ataaaaatta tccagaggcc cattatttct taggcaaaat
    12661 tcatagtagc ggcaaatacg gagtatcttc agaatcagat gaacaaggaa gcggcgaagg
    12721 atatgtttca gccggatacg aatataaaat agcatggaat aacagagaca agtttctaga
    12781 taaacatgaa aaatttcaat tttatctaga ttatatgaaa tatctaataa accgttttga
    12841 aattgagtct tttaaaaata caaacctgac aacagattta gatacgtgtt tggaaatttc
    12901 aaaagaagct cttaaaatga gtccttctaa ttttgatgca cttcattcca tatcctactc
    12961 ctatttctta tattacttaa gaacgagaga cacccaagtt accgttgact cagaagaaaa
    13021 aatggctgca gagaggatca atattagaaa gaaacaatca gagtatttag aaaaggcgca
    13081 agatacactt gaaaaagcgt taaaaattag acatcaagat cataagattc aagcactagc
    13141 aattctattg tattttgaaa aattaagaga gcttccaaat agcgaatcat tgagtcaagt
    13201 aacagaacga aaccagttaa gaacaaaaat agaagcacat ggaaaacaat atctaatcta
    13261 caaaccaaaa tcagataaga aagataaact tgaaaaacga ataaaggata ttctatatac
    13321 agcaaaaggt ttttgaaccc tacacttttt gaatactaat tcatgaaaag aacatttatt
    13381 tagaaagaaa gaaagtatta cttgcaatta aaatctaaat atatgtaaca tgatttattc
    13441 gagtaaagct agaataatta aataaagtca tgagtgaaat agattcaaca gcctcaattt
    13501 gcatcctatg caatgaagaa aggactccag atggtatcac tactaatgga gtttttaatt
    13561 gcataacctg tgggaatgag tggatattag aaaaaagaaa atcacaaaga ctagcaaaaa
    13621 ttcagttcac tggggattat tcaaatttaa ttactgaatt tgttacaatc ttcgattcca
    13681 atctaaacct aagacaactt tgcgataaaa cgttatctat tatcaaaaca aaatttggaa
    13741 tcgagaaatt ggggtttatg attcatgaac ctgtaaatca gttttttaaa tttgaaaaat
    13801 acgtagggag tgaaaacgct agtaaagtaa ttactaaact aaaaacaaac tcagaagata
    13861 caaatcatcc ctttgcaaaa tcaattctaa gtaaaaagcc ctcgattcgt tttctaaatg
    13921 catccaatcg ttttgataaa atttacacac gggttacaga aggtgtagtg agtcaaatcc
    13981 tagtccctat tgtttataag tcgatgcctc ttggcttact ggtttttgac tttgatgaca
    14041 aaagaatagc agaggagaaa tttctagata aagatgtatt caatgttatt tcgaatcaag
    14101 ttgccattgc actctaccat acaatcattt ttaataaatc gaatacaaaa tataaacaat
    14161 ttgttaatct tcattcctct ggactaacgc tcaacaaaca ctatcttaat aatacacttg
    14221 aaattataaa gatgtcactg cttacaatct ccggattagt agaaactgat gtaaatattt
    14281 tactgattca taataaaaaa tttaaagtga ttacctatca taaattaatt agaacgatag
    14341 actccttaga cctttcgcaa gattctattt taatggacaa taatgagccc tataaagcct
    14401 tttttaataa acaaacaccg acacttttag agacagaaaa tgaaccgcta tctaaaaaat
    14461 tgggatttac tggaaagcaa atcttaaatc ttccctcttt cactcttgaa gtaaatgagt
    14521 atgttttttt attaggaaga aattctacta tattgtttac tccagatgaa gttgaagtat
    14581 tagctgctta ttccgaactc gttaaaatta caatcgataa tgccttttta tatcataaga
    14641 tggcaaagca agaaagactg gaaaaagaag tagaaattgc gagagaaatc caactgaatt
    14701 tattaccaag agaaatgcct aagcatccaa ggtatgaatt tgctggcttt atggttccag
    14761 ctagggaaat cggaggagat tattatgata tattagtttc tccagatcaa agtgaaacgt
    14821 tatttgcaat tggagacgtt tcagggaaag gacttccagc tggtatggta atggttacgg
    14881 caagaacaat tatccactct gttattcgaa gaatcagcga attaaaagac attgtaatgg
    14941 agttaaattc ctatttatac tataactcta agaatgcagc caccatgaga ttcatgtctc
    15001 tcacctgttt aaaatggaat gttgagtcca atgtttttca atattctggc tgtggacatg
    15061 gtaatctact cgtttaccgt tcagataaaa atacagtaga agttattttc actggaggga
    15121 ctgttcttgg aattacagat gatatttcac ctatgtattc agaaggaact atagaattag
    15181 gaattggaga ttcgattctg atgtatacag atggagtgac agaaacgatg aatactcgcg
    15241 gagaacaatt agaggaagaa gggttaattc gatcttttaa aaaatataac aaagaaaaac
    15301 ctgcggaaat attagaaggg atatacaaag atttaaaaga ttattcagta aatgttacat
    15361 ctcagcatga tgacatcaca atgttaatta taaaaaggtt caaatgattt ttcaggattc
    15421 aacatacaaa atatttctag aaattttcaa tcgacttggt tcctaccaaa aattaattca
    15481 aaactataaa gctatcattg gattttctgg gggaaaagac tctacactac tcgcatattt
    15541 ttataaattt ttaaaccaag aaaaattatg cccagaacca attttgtttc acctagatca
    15601 ttccattcga aataattttg agcaagaaga agagattgaa ttgtatatga aagatttttt
    15661 ttccaactca atctataaaa aaaaaacatt ccaaaaatcg cgaagtatct aaaaaaaagt
    15721 ttagaagact caggaagact tgttcgttat aaatatttaa atcgttatgc aaatcaattt
    15781 aatggatata ttgcgacagg ccatcaccaa aatgattatc tagaaaccat tctcattcat
    15841 ttaattcgag gtggcggata ttctgcatta tcctccttac caatctataa aaataaaatt
    15901 tttagaccac tacttgtctt cacagaaaac gaaatcgcta cgattttaga atctgaaaat
    15961 tggaaaatct ttactgatga gacaaatcaa gacaatagtt ttcttcggaa cagacttaga
    16021 aaccaagtta taccaatctt aaaaaatgct ggcataaaaa taaataaagt ctatgaaaac
    16081 tttcatgaaa gtcatttata tgatttcgaa gaagatctaa tcaaacaaaa aaaaatacct
    16141 agctatttaa agttagatct aacaaatcta gaaaatatcc aatcttttga attaaaaacg
    16201 ttattagata actattccaa tattttaaaa attcatcctt tcactagacc tattctaaat
    16261 gagttactaa gaaatataac taaaactttt gaaatagaaa ataaagaagt atatttatgg
    16321 aaaggatcaa atgggatttt atatctagtc cccaaaaact ctcccgtctt tcaaattccc
    16381 aaagtatcct acagagatca aaaattagaa attaaatgga attcccatac acaatattat
    16441 tcagattctt ttcaagtggg ctcttttcaa aacggtttaa agattcagaa aaacaatatg
    16501 agaaaaacag tttccgaaat tttaagaatg tatgaaattc caagaagagt cagatttaaa
    16561 attccaatgc tatttagaaa tcagcaacta tgttccattc tatttagttt atgggatgac
    16621 aaattaaagg attttcaggg agacttatat tatgaagcaa aataaactag tgattcactc
    16681 ttccgccttc cactcctctc attcggattg gaaaaaaatt cctgaaaatc cgacaatgcc
    16741 acaaatcgcg ttttgtggtc gatcaaatgc aggtaaatct agtttactaa atgcgattct
    16801 tgcaagaaaa gaaattgcgc gtgtatctgc aacgccagga aaaaccagac tgataaactt
    16861 ttttcttcta aatgatcatt attttctagt cgatctacct gggtttggtt atgcgaaagc
    16921 atccaatcaa acaagagacg acctaattaa catagtcaat ggttatttaa atcatgtaaa
    16981 gactctgaag ttattatttg tattatgtga tgcaagtaga ccccttccag atgaggaatt
    17041 aagcttaatc gatgttgcga gtgcaaagaa aattaaagta gccctagttc gaactaaagt
    17101 tgataaatta aatcaaaagg aaaaagcaaa actaatgaaa gagttagagg aaataaaaaa
    17161 ggaatatacc gaactcgata tattcctttc ttctacaaaa acaaatgaag gaattttaga
    17221 attgagaaac ctcttggcag agtattaagc aaatgtatct aacgaattac cagttgcttg
    17281 catcaaccct agcttgacaa gggtttctct aaaattgctt tgtaacttag taatcgtctg
    17341 ccagatagat cttccttttt cagaaatttc tttgtttctg agttcatcca attgaacagc
    17401 aattctgctt tttgtttctc ttaactcttg ctctctagct tcctttttaa tatcttcaga
    17461 agttttaaaa gcagggtttg agtaataggt ctttctcagg gacatgtcta tttcagtttc
    17521 gatttgttct agtttttctt tcaagcgact ttcttccatg ttgattccat cacctgaaaa
    17581 ctcagctttt ttagcagtat gaatgtcaac tatatcatcc gttaaagcag gaacagcctt
    17641 tttagatgtt ttgcgagttg gctcctcggt tggttcagaa gtagttacag ttgtctttcc
    17701 tccaactgct accattttac catctcttaa ttcatagtca attttaacat ctaaactacg
    17761 aatttcagcc ttatctcgga ttgctttaga tttaaactca gctacatgat ccaattcatg
    17821 cccaataaca tgcataactg aagtgctcgc tggcgatttt tccaaaaggc catgaccaat
    17881 ataatgaata gaagtatcat tctcttttgg tttctgagaa aagttttgac tctggtttat
    17941 tgaatttatt cctgttgtca tcatactaat atcctcgaac gaatcttttc atttgtgtag
    18001 acatttttac agtattgtaa ataactttac taaaaatgca acaattttct gatttggcta
    18061 ctttttctac cataaaaaag aaaaaagtag cgctttttta ggaaaattta agactttttt
    18121 ctttaaagag aagtatatcc ttcttttaga aactttccga gttccttttt ggattcaaaa
    18181 ttacttaaaa aaaccttaaa atcgtattca gacatatttg ctttttttaa accttctcca
    18241 atagcaatat aggttaatgc atctgtctgc cattctagga gaccattttt ttgagccact
    18301 gtatttaaat ctttcttaaa ttcatgattg aagccagttt gatagtaaat agcggttagg
    18361 tccctcacat ccattttata ggtctgcatt tgtgctttac ttccacccga actagaacca
    18421 ctgatagact gaattgagcc tgatagactt tggacagaat ctgaaatttt cgaaatagag
    18481 tttgaggatg tagaaactcc attgagcgag gtggatgcag accccaccga ttctgaaact
    18541 tgacagttta ttagtaaaaa taaaacacta atcaaaattg gaaaaataac tttcataaga
    18601 tactccttaa aaaattttct catacattaa cccaaaatcc aatcgaattg caacctaatt
    18661 ttttactcca atcacaattg cctgaaatgg cttcaacatt actttgattt ttttaaggtt
    18721 gttatctact tttaaactgt agattttttc tccagtccaa taatcatttg aatcaatcaa
    18781 tttcacttta aatggaatat ctaattcgat ccaatcgtct ttaggggttg ggttccaaac
    18841 ggcaaaaaaa ccattctcat ttataaatcc acggggaaat gaatttccaa acatgccaag
    18901 aggaatcgat ttcttttttt ggcaaagctt actaagatcc aatgcttttt gtaggatttg
    18961 aactcgatcc tcttcaattg taaccaaatt atcgctaact aataaaattc ctccactcat
    19021 tgccataacg gttgccataa gaatggtttg ctcaggagtc attttattct tctcttttcg
    19081 cacgatcaaa cagtcaggat catttagcca tagatttcta tgcataaaac ttcgattgat
    19141 tgtattaata agccctttct cggtagataa cgcatgttta tctcttagca ttcttctttt
    19201 gatttccgga taccaaaaag gtgttacatc acagccaatt cgcatcccat caaaaattcc
    19261 aatcgatgga atcaaagggg caccacatcc taataaaaat gtattttttc caactacttt
    19321 tcgaattaac tcaagtgctt gccggtatct ttcaaaaggc gttaatcttt tattataagg
    19381 aacacctggc agagaagcag agaataagaa atctaatttg agataactat aaccataatc
    19441 tttagtaaag gtcttaaata attcctctaa aaattcaatt gatttaggat gggttacatc
    19501 taacgtataa gtgaaatcaa gaccccaaag tggattccaa attgcaggaa caggcttgcc
    19561 ctttgcatct tttaagattg cttcgggata aagtgtaaaa aatgaactct tttgtctgac
    19621 taaaaagggt gcaatccaaa ttcctggcgt tagtccttgt tttttaattt cttccgcaag
    19681 aaatcgaact cctcttggaa atttttcatt tactacagtc caatcgccaa tttcattttg
    19741 atatccatca tctatttgaa aaaattcaat tggaagcttc ctatttttaa cttcatgtaa
    19801 attggaaagt ataatttctt cagatatgtt tgtataataa taataccaag agcaccaacc
    19861 agtgggcaca ttttcttcaa atgttttgac ctttgctttc tttccgagtc cgcttgcata
    19921 ctcctctaaa atcaattcag gtggagtgtc attaaataaa tccaagcgaa cttgtgtcag
    19981 ttcaactgga gtattgtttt taaattcagg tgtagaatag aaatcgtata taatagaaag
    20041 agaatttatt ttatttgcat cccaaagaat atgaaatttg gtaaattgac tgcctatttc
    20101 attgactccg attagaaagc ctttttcttc tgtttttgaa taaccaatta aaagaccttc
    20161 gctcacaaaa tttccaggtt tgcctgcatg gtctgtataa atattttcct gtccatactt
    20221 taaaaaacct aaaagtctag gcgaatagtc tttgtcttct gatgaatagc gagtggtaaa
    20281 actccaagac tggtagccat gctgcagaat atttaaattc gtaaaggatt catcaaaatt
    20341 caagtcaatc cgtaagtcta aaatcacaag tcctatagaa ggtcgttgat gatcgatcca
    20401 agaaagactt ccaataaaaa gaatcgcacc tagcttatct ttttttcttt gaaattttaa
    20461 ttcaaataag ttatcctcac ttaaataccg attttctgaa tgctttctaa attctgaatt
    20521 gtatatttta ttaaaaattt tatattttaa agtggcttta ttcattttat cctacaacta
    20581 tggtaacatc aatcggatgt aaatattagt ttgatacaga ttggaaagaa gtcattttta
    20641 acaatcattc agtctgttct tcctgtcaaa tataacagta aattatttat gacagctagt
    20701 ataattatca tcttttaaaa ggtatttatc taagtaatta tttatgcaac aaaaacattc
    20761 acccttgaag ccgaaagttt ttcaatctga tattgatgaa gaatactttg aagcatattc
    20821 caaagatact catcttgcaa ttgactgtga aatgatgggt ctaaatcccc gaagagatcg
    20881 tttgtgtctc attcaaattt gtgattccag caatcgtttt agtttaattc aaatcttacc
    20941 cgatcaaaag caagcgccac tacttaaaaa attatttgag aatcctgata ttgttaaaat
    21001 atttcatttt gctagaatgg atatgctttt tctgaaaacc tatttaggta tacaagtaaa
    21061 taaagtcttc tgcacaaaga ttgcgagtaa gttagctaga acctatactg ataagcatgg
    21121 tctcaaagaa ctcatacgag aattttttga tgtgaatata gataaaaaaa atcaatcgag
    21181 tgattgggga aaaaaagttt taacaaaaga ccagttagaa tatgctactg aggatgttcg
    21241 atatctgata tcactcgaac accaactaac agaaattcta attagagaga atcgatatga
    21301 gatagcctct aaatgctttc aatttcttcc caccctaatt gatttggact ttctagaact
    21361 taaggatgtt ttagagcatt gaaaaagcaa gtccgacttt ttatctgtca atcttgtgga
    21421 caggaatact cacgttgggc tggaaaatgc gaatcctgtg gagaatggaa tacaattcag
    21481 gaagaaattg ggcaagagag atttagtaaa caaagtttca aatctcaagt tgataaatac
    21541 agcaaaccaa agcctatttc cgaaataaac gaagaggctt acaagcgtat acccacaggc
    21601 tttaaagaac tagaccatgt tttgggagga ggtattgttc cagggagtat gacgcttatt
    21661 ggtggagaac cgggtgttgg aaaatcaaca cttatacttg agatctccaa gaacatttct
    21721 aaattaggga aagttttata catttccgga gaagaatccg caggccaaat cagtcttcgt
    21781 gcaaaacgaa tgggtataga atctaaaaat atttttatct catccgaaat ctatgcagaa
    21841 aacatcgcaa gtatgatagc aaacgaagag ccaactatcg tctttgtaga ttctatacaa
    21901 accatttcta aagaatcaat tccaaatgca gcaggaacag taacacaact cagggaatgt
    21961 tctcagcttc tattagaaac tgctaaaaaa acaaatatac caatttttat gattggtcat
    22021 attacaaaag aaggaagtat cgcgggtcca aaagttctag aacatatagt agacacagtt
    22081 ctgtatttcg aaagtgataa attaaattat tatagacttc ttcgaggaat taaaaataga
    22141 ttcggggcag taggcgatat agctgttttt gaaatgagat caaatggatt aaccgaaatc
    22201 attgataaac ataatttatt tatctcccct gatcatgaaa ataggactgg ttctattctt
    22261 tccgttgtaa tggaaggaag caggtctctc tctgtggaag tgcaagcact tgtgagtcgt
    22321 tcctcttatt cacaagcaag aagaacagca gaaggattgg acaataaaag acttgtttta
    22381 cttgctgctg ttctagaaaa attcatggga ataaaaacga ttgaatgtga tatattctgc
    22441 aacctcgcag gtggattaaa tatcgatgaa ccagctcttg atttagcaat ctgtgcatct
    22501 attgtatcta gttatatcga aaagccagcc aagaaaaatt atgcagtgat tggagaagtt
    22561 gggctctcag gcgaggtaag aacagttagt cacatccata caagactcaa agatctaaca
    22621 agcattggga taacaaatat tatcctccca aagggaaatt taactgaagt aaaagataaa
    22681 aaccataaaa tagaattgat tccaattgaa agtataaggg aattagaaaa attgttttaa
    22741 acttgagtag aggcgtgaat acaagcccct actctttcct ttttcaaata aaagctatcc
    22801 tatatttcac ttggatgctt tatgtaatag agagaaagcc agatagtgcc taatagcact
    22861 aatgagtaag cggctaacgc gctagtaaaa tcaacaattg gaagaattgt tgcgataatc
    22921 gcgattttag ctgctgtaag cctagtggct tctcctaaaa gtaaaaagat tccaccaagt
    22981 gcaattaggt gaaatctagc cattgtctct ttaccaactc ttgcgaatct tgcaaatact
    23041 ggaacagcaa gaagagccat tgcgaaaatt gttatctcat tcatactgag cctccttggt
    23101 atagatgagg ataacactga tttggcgaaa aagaaagaat ttttttacta cgaatcaaaa
    23161 aaagaaaagc tgcacagttt tttaaaatca atcgtttctt gtcaagaaat atttaatttg
    23221 gagcaactga tctgaaacaa gtatgtaaca aaaaagaaac agaatataaa ataaaaaact
    23281 ttttttattc aatgtaagta gagagatagc agaatggaaa taattcaatt accaccagaa
    23341 tgcttttgga gttcctttgc tccgcctact atcaaatatt gtgaagaaaa tatttgctct
    23401 atcatcacac agccatcgaa cacatggtcg aatttggctt atatatttat tggcttttat
    23461 ttgatttgga aaaataaaaa cgatagtata tcttccttaa aattagttgg tcctatggca
    23521 atcgcaaccg gtatcgcttc ctttttatac catgcgagtt tcacattctt ttttcaattc
    23581 tttgacttat cttctatgta tttattttca acgatgatct taagtatgaa tctaaaaaga
    23641 gctaagataa tcaaaaaaga atatcaacac ggaacacagt ttattattct agccgcttca
    23701 atgcttctcc tttatatttt taagccattt ggaattccca tcttcgcctt acaagtatta
    23761 gccggtctaa ttttagaatt ctacattttt agaaaaatga aagaagaaat aacttattct
    23821 tattataaat tatctttatt attcttagta ttagcatttg ttgcatggac agttgatttt
    23881 agaagaatta tatgtgatcc aatgaatcac attttacaag gacatgcagt ttggcatatt
    23941 ctatcttctt cttgtttctt atttttatac aaattctatt cacaggaaaa attagaaaaa
    24001 acttaaagat taccgcgcct cgactaacga cttcgcaggt ggtataaata ttctcacttc
    24061 atccaatgag agactttata agaagcaagt cttataaagt ctcgtaagaa atttctatag
    24121 tttcgaatca ctgcttgttt gttttctttc gctggattaa aaattccacg ctttcgaaag
    24181 agttttgatg gttctaatgg aatatcagac caagttccta tctctaaagt ccagggcaga
    24241 aatctcgatt ttaaattttc actctcaatt ttattttgat attctaggta gagtaaatcc
    24301 cataaatctc cgtgagttgt ataagtctca ctctgtggac catactcata agcactatgc
    24361 aaattctttt cttttaaatg ccacgccatc ttcttatata ttttttcatc cttgcaagcg
    24421 tctgtcgtaa aagcaaaagg ccaccacaca tgatctatcg atccaaatcc agaatgaata
    24481 tctatcaatg gcataattgc tgttttgtta ttaaaaaaat acttcttcgt aaatcgatag
    24541 gcggcacgtc cttcagactc tagataattc cctctaaaat aaggaagatt agaactgatt
    24601 ctgtgccccc caaaaaagaa cggagcttta atcgcatcta ctcctgaatt tcgcatgaga
    24661 tctacaccat ttggattaga gcgagtggac tttgcgattc cgcccggatt aatgatagga
    24721 atacttacaa ttcccaaatc tccattaaaa atttctggaa gaaaatcttt acggggacta
    24781 tttactaaaa attctaaaaa atcaagaatg actcatactc caattgtctc tagaccatga
    24841 acccctgcca caattccaac tggatttttc tggattgctt tttcttttcc aatttctaaa
    24901 gaataaattg gaaaacgaaa attgtcttta gtctttctag aaaatcccaa ttgcgaaagc
    24961 cgaacgagtt tgcctccatc tttcacaatt ttaaagattc tgttttcata tcgatttaat
    25021 cgacgaattc cttttaacat gaagattatg cttttaaata atgtttacca taacgctcat
    25081 cgatttcttt tctatcaaat aaaacaaata catctgttgt tttgaagatt ctgtctaatg
    25141 ctgggtaacc acagattttt gctccaagtc gaacataacc tttaatgata ggtggaattg
    25201 ctttattcac tgctttccaa tccgccacct tataattggg atcaaaccca actaattgat
    25261 aagactcata aggagttaca ccgaattctt ctcctgcaag tgcattgttc tcttttagat
    25321 acgcataggc ttgagaggca acgattgaat ccatagagtg aatcgatccg cagcccatta
    25381 gatagcgaat atcgtttttt agcatatatt ccccaaatcc agaccataag agagaaatca
    25441 cactcccgtc cctataatct gcatgcacac aacttctacc aacttctgcc acttcatctt
    25501 tgaactcata gatttttgat aaatcaaatt cattttcaga gtaaaagcct atatttgcct
    25561 ttgcaacaga tctctttaat atgcgatagg tcccaactat cttattcgcg gtttcatctt
    25621 ttactattaa atgatcagag taataatcat attcatctct gtctttctgt gcctgtcttg
    25681 attccggtaa gccttccccc atttccaaat taaaaacatt gtatctaaga gataatgttt
    25741 gttcgatttc atattgattt tcagccaatc tgacttctaa ctttctttcc acgccttgct
    25801 ttttgctgat tacactaata gccatatgtt atgattactc ctctctttat acttgtataa
    25861 tctatgatag atgttacagc attgttacgt agaaaataaa aaaagggaat taaagaatac
    25921 cagcgattgg gacgatgaac accgagagta ttctgtttcg acatatagag aggaacattg
    25981 agactatcga ggtaaggggt tgttataaac gcctaatggc atcaaagata atttcatagt
    26041 taagtttgtt ttgttcttac cagtgtcatt ttcgtgctca acgcctaatg gcatcaaaga
    26101 taatttcatc atacctatac agaatttttc cttggtcgac gtttgtgctc aacgcctaat
    26161 ggcatcaaag ataatttcat ttctttggct tatcttgaat aaagagcatt tctttatgtg
    26221 ctcaacgcct aatggcatca aagataattt cataaagcta aaatagaatt gtctttaaga
    26281 actctataag tgctcaacgc ctaatggcat caaagataat ttcataaaag aaagattctg
    26341 atgtagttaa cgcaccagat gagtgctcaa cgcctaatgg catcaaagat aatttcattt
    26401 gcaattttct aattttatta aaatttcttc tatactgtgc tcaacgccta atggcatcaa
    26461 agataatttc atataaatac agtgtctggc ggtataatta ctacagatag tgctcaacgc
    26521 ctaatggcat caaagataat ttcatccgga acagaaataa acagtgtgta taaatagatt
    26581 agtgtgctca acgcctaatg gcatcaaaga taatttcatg attgagagat ggaaggtttg
    26641 ctatagctat aagaagtgct caacgcctaa tggcatcaaa gataatttca tttttcttca
    26701 ttctcttttt ttgtgaaatc tcttagatgt gctcaacgcc taatggcatc aaagataatt
    26761 tcatttttct tcattctctt tttttgtgaa atctcttaga tgtgctcaac gcctaatggc
    26821 atcaaagata atttcatcgc taatagtgcc gagcattacc gccccgccta acggtgctca
    26881 acgcctaatg gcatcaaaga taatttcatt atcacttggt tcataatcta ctaaacattt
    26941 ccctatcgtg ctcaacgcct aatggcatca aagataattt cattttattg cattttcttt
    27001 gtcggcatct aggctcatat gtgctcaacg cctaatggca tcaaagataa tttcatttca
    27061 actatttgtt cttcaactaa cgttccacct ttgtgctcaa cgcctaatgg catcaaagat
    27121 aatttcattg tgtctgtgcc tctgcgagtg tccaaggtga gtttgcgtgc tcaacgccta
    27181 atggcatcaa agataatttc atctttacta ttagactaag tggtatcaaa aatgataggt
    27241 gctcaacgcc taatggcatc aaagataatt tcataattat tctctctgtt ggtctgttta
    27301 ataatctgag gtgctcaacg cctaatggca tcaaagataa tttcatcatc taactcaaac
    27361 ccattcaaag cacttgtagc catgtgctca acgcctaatg gcatcaaaga taatttcatt
    27421 tttcttcctt cttcactaaa agcagagaac atggaaggtg ctcaacgcct aatggcatca
    27481 aagataattt catttttccc gatttctatt tttgtgaatt ttatttcttg tgctcaacgc
    27541 ctaatggcat caaagataat ttcattttgc aggtttagca atttggcagc ctgaaatttt
    27601 gtgctcaacg cctaatggca tcaaagataa tttcatcatc tattttatta gagccaacat
    27661 cgtttagtaa ttgtgctcaa cgcctaatgg catcaaagat
//
LOCUS       DAOQOS010000074        27680 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_787_length_27680_cov_5.761267, whole genome shotgun sequence.
ACCESSION   DAOQOS010000074 DAOQOS010000000
VERSION     DAOQOS010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 27680)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27680)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27680
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_787_length_27680_cov_5.761267"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..285
                     /locus_tag="PLF66_15830"
     CDS             <1..285
                     /locus_tag="PLF66_15830"
                     /inference="COORDINATES: protein motif:HMM:NF028171.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase C-terminal
                     domain-containing protein"
                     /protein_id="HMZ65563.1"
                     /translation="MLSICTVKEEYKPQLPAITHVDGTARVQLVNQKTNPRFYKLLKM
                     FGEKSGIPILLNTSFNLKGEPIVETPLNAIKTFEWCNMDALVLENYIIKK"
     gene            291..1589
                     /locus_tag="PLF66_15835"
     CDS             291..1589
                     /locus_tag="PLF66_15835"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HMZ65564.1"
                     /translation="MNKYEIEKILFILCSENIEIPHIQRVVKNIRYRFPNFKESVFTT
                     DDKFFDHYSKNKELNLVKSSLNAYNEFKVVVLILGNNNERILQEKIDYNRTRVYEADS
                     LGMIRYTKLELDDIEYPFENRHIFNQIASNLGNERIAYFPYSYITQYVGLGPINQFGH
                     RVDPKEINAIQNREKDTKLIVITGGSAAWSIHTNYDEMFANCLEKKLNSENLKYKFKV
                     LNFAQPSAVILNNIFDYILHVQRLKPDLVIFHSGANDLAFGQTSDELLVGEHSIIYQF
                     QFEDWAKILETDRNYNPKNPKITFKEIKNYPRTIIGALKERILQFKTIVESTGGKFLF
                     GLQPMSFSKQAISTKEKESLKKYSENFFKPIMDNLNFLFNKCEYYLTNDKSIELINFH
                     KEFNQYNESYTLFTDQVHTTPDGDSIIADIYFEQIKRYWS"
     gene            1603..1752
                     /locus_tag="PLF66_15840"
     CDS             1603..1752
                     /locus_tag="PLF66_15840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65565.1"
                     /translation="MSFIRILIQLIFRGFFFDFLVMSYFYIFKKDQFISNKEVEANPF
                     IYMMQ"
     gene            1855..2679
                     /locus_tag="PLF66_15845"
     CDS             1855..2679
                     /locus_tag="PLF66_15845"
                     /inference="COORDINATES: protein motif:HMM:NF016908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HMZ65566.1"
                     /translation="MNNFLSKFIRDNINEQFFIFDIGAYKGEFAEQFVEKNINSIIYS
                     FEPDKNSYTALSEKYKKNSFIYNLAISNYEGQKKFYQQKKGYRSSLLKDNYSEEDTEI
                     IDVKVTTFDHFMNQEKIEIRENSKIIFKTDTQGNDLNVLKGAESFLKKYKPIIITEVI
                     FQPLYDSQCHYLDILNFLFSIGYELVYIDGTHLNTDEKISYSDYYFQIKEDSKSDKAN
                     SSFHLHIEESVNKELEMYKTAAKERLDLIQRLDQECNDRLKVIEWQQKEIEKLKSK"
     gene            2688..4391
                     /locus_tag="PLF66_15850"
     CDS             2688..4391
                     /locus_tag="PLF66_15850"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HMZ65567.1"
                     /translation="MGAVNFSLDKNLVKTIQNFSPVDTLVETGTFEGDTIRNLLNDFK
                     TIYSTEISIEYFDRAISDFKNHKHVKLYQLSSPEFIKKIQPDIKNNSSIYWLDAHWMV
                     ADFAAGEKSQCPLLEELDAIGELNNDSYIFIDDARLFLTTPPYPHEKSHWPSFDSIVN
                     KLQSLSKKHKIMVLNDTILFYPNKSEEIINEYAYKHSIDWLAILHKSRDYDNLRVQFD
                     GLMPQMEKLEAEILQSKQKDVLIKSLHRFANELQQELKRKQIPFETQFAMLPGNLLSI
                     IIPTYNQGQYIEDCLKSILNQTYQDIEIIIQDSNSTDQTESICKKYATIDPRIQYYRE
                     KDEGQSDAINRGLAKAKGDYWTWICSDDRYHANPDALKNLIQHFFEKHSESSKVVGVY
                     GNAFFMNETGEILHEYGNFKKDLTVEHFIHNWPLSQPSSILLTKAVRQVNGVDKNLFL
                     GMDLDLFIKILKNGNQFVFVDTPVVDIRLQDNSKSVKYRKDTALNALNLILSHFNTIG
                     NYQESAYFKELFLTAETTEIRKFLSKLNWKFKWKYRVMNLLEKTSITQKLLHLLRKLR
                     G"
     gene            4393..5328
                     /locus_tag="PLF66_15855"
     CDS             4393..5328
                     /locus_tag="PLF66_15855"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HMZ65568.1"
                     /translation="MAKNFTVIGIGGYIAPRHLKAIKETGNHLISSYDRNDSVGIVDS
                     HFPDADFFTEFERYESFNEKQIREGNKIDYVSIMTPNYLHFPHMNYALKIGADAICEK
                     PLVLNPNEIDTLIELEKVYNKKVYTILQLRHHQIIIDLKNKIDNRKSDSKIDIDLTYI
                     TSRGKWYFESWKGDVRKSGGLATNIGIHFFDMLTWIFGKLESNEVHLNEPAKCAGYME
                     LEKARVKWFLSVDYNDVPESQKKKGQRTYRSILIDNEELEFSEGFTDLHTVSYKEILE
                     GRGYGLKDARESIVIASQIRNLPITKTSNKHRFVK"
     gene            5328..5918
                     /locus_tag="PLF66_15860"
     CDS             5328..5918
                     /locus_tag="PLF66_15860"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HMZ65569.1"
                     /translation="MSDFFIHETSLKDESAVIGENTKIWHFSHILKNTVIGKNCSFGQ
                     NCVVGPNVKVGNGVKVQNNISIYEGVEVEDNVFLGPSMVFTNVINPRSFIVRKTEFKK
                     TLLKKGCSIGANATIVCGVTIGRFALIGAGSVVTKDVPDFGMFYGNPGRLQGFVSIAG
                     NRLNFTNNRAVDSYDGTEYELRDGLCYVVNAGSYET"
     gene            5908..6996
                     /locus_tag="PLF66_15865"
     CDS             5908..6996
                     /locus_tag="PLF66_15865"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HMZ65570.1"
                     /translation="MKLNFNDLKTQYNRIKVDLDKSIQNVLEKQNFIMGEEIEILEKK
                     LSQFTGAKYSISCANGTDALQIALMAIDIKPDDEVITTPFSFISTVEVIALLGAKPVF
                     CEIDEKTYNIDATKLESLITKKTKAIIPVSLFGQASDMDTINQIAEKYNLTVIEDAAQ
                     SFGARYKNIRSCNLSKIACTSFFPSKPLGCYGDGGAIFTSNDELYAKIKSIRTHGQEK
                     KYYYQYVGVNSRLDTLQAAVLNEKMKLFPEECELREKVGSRYTELLKTKKNLITPFIS
                     KDNHSVYAQYCLRAENREEIMNHLKTNEIPTMIYYPHPLHLQKAFSYLGYKTGDFPIT
                     EKISKEIFSIPMSPYLTEKEQDLVVSYL"
     gene            7132..8217
                     /gene="wecB"
                     /locus_tag="PLF66_15870"
     CDS             7132..8217
                     /gene="wecB"
                     /locus_tag="PLF66_15870"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HMZ65571.1"
                     /translation="MKKILTIVGARPQFVKAAAVSREFKKHKDIQEVIVHTGQHFDKN
                     MSDIFFEEMEIPKPDYNLNINGLGHGAMTGQMMEKIEEVILKEKPDFLMVYGDTNSTL
                     AGALAAKKIHVRVIHIEAGLRSFNMNMPEEINRILTDRISDILFCPTEVSIENLKREG
                     FENFKVQIVKNGDVMQDAAIYYSKISSTKSRIIETLGLKDQKFILSTIHRAENTDDLE
                     KLKSILSALNQINKEITIVCPLHPRTHNIIKKNNLSTEIKIIEPVGYFDMIELLKNSV
                     LVMTDSGGVQKEAFFFEKPCITMREETEWLELVESGYNVLVGSDSKKIIESVESMKSK
                     KLDFSKDLYGKGKSCSIIAETILNYKN"
     gene            8235..9539
                     /locus_tag="PLF66_15875"
     CDS             8235..9539
                     /locus_tag="PLF66_15875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HMZ65572.1"
                     /translation="MKEELIKKLNNKSAIIGIVGLGYVGLPLALRYSEEGYKVLGIDI
                     DEFKVNEVNFGRSYIEHITSESIQKANKVGFSATTDFQRAGEVDTLIICVPTPLNKYR
                     EPDLSFVTDTVDALLPFIRANQVMSLESTTYPGTTDEELKPRIESKGFIVGVDYYLVY
                     SPEREDPGRKDFNTKTTPKVVGGFSPDCMEVGKALYGGVIDKVVPVSSTRAAEMTKLL
                     ENIHRSVNIGLMNEMKIVADKMGIDIFEVINAAATKPFGFVAYHPGPGIGGHCIPIDP
                     FYLTWKAREYGVHTRFIELAGEINSSMPNYVVDKTIEALNKHGKALKGAKILALGIAY
                     KKNVDDMRESPSVFVMEILRAKGAIVEYHDSHVPVFPKMREHHFELSSVPLTVENLKQ
                     FDAAIVLTDHDDVDYDLVQKNLKIVVDTRGRYKGSFDNLVKA"
     gene            9667..11628
                     /locus_tag="PLF66_15880"
     CDS             9667..11628
                     /locus_tag="PLF66_15880"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HMZ65573.1"
                     /translation="MDFQKSDRKNLILLAVWSLFLFFIAKTFRAYYSMDIPPAWDNLA
                     YQLEGVKLARSFFEGDSSVIRSYFGDNVPVGYIFLLSVSYSVFGFLPESPYIVSALFG
                     FGNLCFIYLISIELNVKRIYALLASLFISTLPNFIYNNFLQTRNDFALSFFILFFLYL
                     LIISLKQKNVWLVFIAGIVSGIGTLFKLSAPGYFIWMYLLFLLIPNDRNDFMTRLKCF
                     LIAGLGAIVTCGWYYIPSLKKILAYYSMWGSFSQTQYKLFSFSDRFLFYFKNFISTHI
                     GSNSIAIILLSILIVGILLNLIIKSYRDKQRELKESFQSSYLYLIVGSFFSVILFLTL
                     NRSFSEAGDTPVLSILFILILVILNNLYSQIRVRKMILILFLVLPISLGNSFSKIAKE
                     KLYSISEFETFVNQIQEFRKKYGMEEEKFLQVFSHPVYNTNAVAWYLHLTNRVPLNSL
                     IKASIEIADWQSLSVSHDVAEMVRVVNKYPFIIVSNENPISFGGEYFVQVNLLNSEIK
                     RQIFESGNYMCEDSFEIGKSKLPIRFCINKTYRQFKFLNKTTDGWLEWDAHASYYSSV
                     SAKFLIKGIPSRDIKAFYLEDIRTKEKFNAKFISVVNHSEYQYEILLPPSTSVRTFKL
                     LASNEQMVPASKEDIRKLALLNTKIEFVE"
     gene            11629..13263
                     /locus_tag="PLF66_15885"
     CDS             11629..13263
                     /locus_tag="PLF66_15885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65574.1"
                     /translation="MRKLKLISTSLTILILVCYIAILAVYQKSLDKNAIPWHDGSHHF
                     TLAANLFNHLSSGDFEYYLESLRNFPELILTYSILNLAFLLVGGVKQAAIPFVYTLFF
                     ILIVLSYFLSISKRERHTKSLLFTIVFILSGIYTTQAGGALDTRVDILSASAGAISLL
                     FITSRNFYFGILFFLFASFLKGAAFFLILPMLLIGIAIEYLKEKITLDWNLKFLLKSI
                     SIAIPLLIFLKVLLASVLSYNLMQTGGSNLESRVQIFISNVNQYLIGGFGYYYKVLKE
                     NTNVVFIFLGIIVAVLYSIWQKNKKLILSGFFVFFCISYSYVLMTMSPLKSGVLIIWF
                     LPCTIVASIFFTDFIFKIRYNKYLILALLSITFIVLYKIPRKYNIPEDYLQLARNNIH
                     QQASEIAIQLDEKYPSRFVKGVVFVNFLSAHGVISYNYDSYTNLIHKKVKKAKIDLAG
                     WELATYSKEWKKEIERFKDYDLILLILQEEPMGIPATNTPQKYGREVYLELEKSRTEK
                     PSCFTKITNTIDLPHIGKRDTYLFSNVPDCKAILFP"
     gene            13269..15530
                     /locus_tag="PLF66_15890"
     CDS             13269..15530
                     /locus_tag="PLF66_15890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65575.1"
                     /translation="MIFLIQYILCFWGIYYIFSGAGEVLKKFLKLKGSPFIWIQKIET
                     ILYGIGFVHFTSTILQLYFSHSVSSIVYLLFLIVLMFLGIYFSIIQSKTKIQFAKLSF
                     VHFLLFLFLLLPFRNFSSEFMASEESGDHTIYLGPVKAQYIQNDSEITKSKWKLALTR
                     AVYDSRIHLLFPPIEKIFPMFEAAKKAKWEIDPIDRVILEGFSPAWWALHWGLIGFGF
                     LLPIHPETIYLSILFFLTSLLCLQVGSIGYLLFNEYTESKILVFFLILLLSLSACIAK
                     ITYGHFYLQLIGLVSLLWIFKATLSWEKTRDKKTFYSALVFSFLIGPSFYFMGTVYWG
                     LIVLCFFLFYYKTNLLIGLFHYIRKPSILIGSFFYLPILLVNIGSFLKFLPSTSSSMQ
                     NKQYWDQVLGGTVTGSDAFLNPILGIVTYFQFYPKLPFKEQIDALNFILVPFAIGILF
                     LLIGGIFKSIFFDLKKNVRKNLFLIFVLIGILTINLKSATHYYNYNKASQYTFPFLIL
                     SFPYFYLKLNANRKSFYLLSLILIFWILLLIPAKFRQIESFSSLHEKSMSNYLLLKER
                     LDHLNKNTIFFFGESRNHALLETYMAGNQTIPVGIYPIDSFKKHFHFENLSLNKFMLK
                     DKDSESYTLFDLSRFLIGADEYWVALSSSIRLDSTKIPNSLYLNSIGKVEVFAELKSL
                     KKLIVYTFHSNTKPTVNRERFNSIENNYMQPENLQSIETVCAKNECKYTYHLEPLGKN
                     FFLIENASRVEVF"
     gene            15577..16593
                     /locus_tag="PLF66_15895"
     CDS             15577..16593
                     /locus_tag="PLF66_15895"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HMZ65576.1"
                     /translation="MKKKIMITGVAGFIGSNVCKHLLQKGYSVVGIDNLSQSDGKNMN
                     EFKENSNFEFYRVDIMNESSIVNAARGCTGIIHLAAYKIPRYSDALDTLKINGIGSEN
                     LIKAAIENNARLVAASTSDVYGMNPELPFAEDKSKLVMGGPKVRRWAYAISKMLEEQM
                     IFAYGERSNLDFSIIRFFGGYGPNQNLTWWGGPQSVFINKALDDEEIEVHGDGQQTRS
                     FTFVGDHVDGVTRCLEVEKARGEVFNIGNDREITIENLAIMIWNLIRPGTTPKIKKIP
                     YETFGKYEDVMRRIPDLNKSKSILGFQPKVPLEEGLIPTIEWQVKRRRELGMPEPKSG
                     SVFK"
     gene            16593..17348
                     /locus_tag="PLF66_15900"
     CDS             16593..17348
                     /locus_tag="PLF66_15900"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HMZ65577.1"
                     /translation="MMNKKIFIITPVLNEEPNMERLLSGWKSIQKDLKDFDFEFILID
                     DGSTDNTVSKAKSVKENLNLTILSHEKNQGPGYAFGTGFEYLAGKLQPHDLVVTMEGD
                     NTSRIDTFTKLVYRQLREGDDVVLASPYAYGGGMTNTAWYRIILSHIANGMVKELIGI
                     HGIHTMSSFFRVHTGEIILRLQSKFGKRIIERRGFESMIEMLKKLILVNASMSEVAMK
                     LDTSLRAGKSKMKIMRTIRGYFKLFMISRKWKV"
     gene            17360..18226
                     /locus_tag="PLF66_15905"
     CDS             17360..18226
                     /locus_tag="PLF66_15905"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HMZ65578.1"
                     /translation="MKIWILLPAFNEEESLPRLLPKISKFCLDSNFNYEIVVVNDGSL
                     DSTLSILQDYSKTLPVTIVNHVINRGLGETERDGFEYIASKIQEEDILIRMDCDDTHE
                     PMYMPSHVEKMREGFDVVISSRFQPGGGQLGVNGYRAFISACANIFMKVLFPIEGVKD
                     FSCGYRSYKGKIIKDAIKVFGNGFIQLKGLGFTSTLETIVKLKLLGARFSEVPFVLRY
                     DQKVSSSKMISSITTLGYLTMAILYHWPFSGWRNSYKGLAKMYRENPEQAILKYNRFA
                     IKRGLTTRIGGG"
     gene            18229..20103
                     /gene="asnB"
                     /locus_tag="PLF66_15910"
     CDS             18229..20103
                     /gene="asnB"
                     /locus_tag="PLF66_15910"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HMZ65579.1"
                     /translation="MCGIVGFFSNNLTKENQIINSMLTSIQHRGPDGHGVFDVDNIVL
                     GHVRLAIIDIQNGIQPFITADNRYVLIFNGEIYNYLELRQTLVSKGYKLTTYSDTEVL
                     LYMFVEYGKKCLDYLNGMFAFAIYDRQSKELFLARDHFGIKPLYYYAKNGKFAFASEI
                     KALLKHPEIQADLDENSLYEYLTFQMVLDKNTLFKDIYKLNPASYMIVKDSKILEEKV
                     YWNLDYTIDESKTIDQFSDELLILLDSSLSIQMRSDVPVGAYLSGGLDSSIVSVLAAK
                     NYLGEFKTFTGGFRESSDYDETYYAKVVSNKIKSQHFEIFPDHNDFLNVYEKLIYHMD
                     EPAAGPGLFPQYIVSKLASENVKVVLGGQGGDELFGGYARYSVAYLEQCIKGAIFDSQ
                     EEGNHIVTLQSIIPNMPLLKQYVPMMKTQFSEGLFDEMDKRYFRLVDRSPSLNKIYSS
                     DFLNQINKEKIFEKFQSIFNFPQTKSYFNKMTHFDTKTLLPSLLQVEDRVSMAVSIES
                     RVPLLDKRIAELSAKMPPTMKFSGGKTKYMLLNAAKNILPTEIVNRKDKMGFPTPTNE
                     WFNGPLKNYVLDHLLGSNSKTKQYFNSANLEKVLKGTEKFSRDVWGALSLEIWFKTFL
                     "
     gene            20100..21422
                     /locus_tag="PLF66_15915"
     CDS             20100..21422
                     /locus_tag="PLF66_15915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65580.1"
                     /translation="MILYIRTDLSDTSWLEYILSEFITIQNAKFEFQIVPLGTTTDEK
                     STLFYTKEKLGKLNWVPNYSNSIVSENLKYIDSRICILENTGDEKNFDIFWNSFVFLT
                     RYEEYLSEKKGKKIQSYSFNHPRKDKESFSLPIVNILFQYLESYIKDRFKTLRFEDTK
                     EMYIEFSHDVDYIKKTIQLRLKQTIFNIIKSLKILLSFSLFWIELKRLFYFLFSTPSY
                     WCFDYWVNLEKKNNIRSIFYIYVKTKTNRGFISWLIDPSYDISNNFELQEKLKHLYEE
                     GFEIGLHGSYKSALDKEQLELEKNILEEILGIKITKTRQHWLRFEEKITPKIHSDLFK
                     FDATLSWNDRMGFRSGIASQHRAYDHENKKPFSHVITPNILMDSNIYDYGITKVNELE
                     KKSLELLQSLDQYKNIYIALSWHQRVCSEDYKWHFIYEKFLNWYSKKR"
     gene            21428..22462
                     /locus_tag="PLF66_15920"
     CDS             21428..22462
                     /locus_tag="PLF66_15920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65581.1"
                     /translation="MKQSKIRFFIFTIFSICFAVGFLWVIIDSIMVWRGFKPLDLSYG
                     FETEFLEGRGYAMRRGTQNYRTFINSLGMRREELPELVPGEKRILTLGDSTCYSSNAS
                     FENSWPNVLERDLRSKGNKITVMNGGVPGYSTMNMYHAFKWYLKRTKIDYVIIHGGWN
                     NYWNDKALVENFDPDSYLKKIFPNSPDLEDVNKNLTVIENIDSSSNLLEHSSGAMKYI
                     ATSSALGHYLVKNIWLTIIDPMLRGSISQGSKVNSVDNKKSITSVNPELAIDDYMLYL
                     GLLIKEARKNNITPIIIKPFSVLRIPGFNALKASKEYPLYMAKDYTWMIPRMKEMDKA
                     SEVFSKYNLA"
     gene            22541..23572
                     /locus_tag="PLF66_15925"
     CDS             22541..23572
                     /locus_tag="PLF66_15925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65582.1"
                     /translation="MGSPSHPFDRGNILLSKILAEKLSEQKILEPAGNVKWVHPKEKQ
                     YLYDLKFREVNFKVGKKGLLFVALFSLFLVISGISIMSFLNQKLSTIQLAISPFISYL
                     VLVSTQYILFQFSEKILINFSFSCFIFIIMIIFSYRKKIREDIIRIISPKVGLSIFLS
                     VLSICFFSLYGFEKLNSITCSTLYNGCRLIQEDLALTDFLFDKDKDSFRLYSLYTLLQ
                     SGEDRPKLLIDIMYNYSMLKQMLPLLEGPLYYFSNMNLETINLFFIFLPIALVSNLVF
                     QILFFERLTFLAKTFAFVFIFTLFNNLPLLFTNISKVWFLGTIGILITSFVSSEKIYR
                     SFFLYYQLV"
     gene            24035..24637
                     /locus_tag="PLF66_15930"
     CDS             24035..24637
                     /locus_tag="PLF66_15930"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HMZ65583.1"
                     /translation="MIRSAKIDDSVAITKIHLETLPNDLLPRLGFSFLKNYYHNLLSS
                     SQALVIVNEDSGQINSFVIFAYNTFELSKLIKKNKIFLAYSILKATFSDFRIPILVLN
                     FIFGKEKYNIKIEEIETIPELYSIATASNYKSKGIGAAITKFGLEILKNKTHRQGCIV
                     KTSSKRAKQFYERLGFLEIGETIRGNNKLSILYIPFEEIR"
     gene            24634..25827
                     /locus_tag="PLF66_15935"
     CDS             24634..25827
                     /locus_tag="PLF66_15935"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HMZ65584.1"
                     /translation="MIPRLKPFMGIDDLFAAISFFKSKSVEKFESEFAKKVNQKYAIA
                     FPYGRTALMILLEAMNLKKKEIICPAYTCIVVPHAIVFSENDPVFLDSNPNDFNMDLK
                     KIEPLLNNDTGAIVATSIFGYPINLDELKRIKKKYPNIKIIQDCAHSFTANYHGEFVH
                     TVGDAAIFAMNISKHMNSIFGGMVTTDDADLAKKLIHLRDSKLKSPPFKKTLFRLAYL
                     FAVYPTFHPFLYGIVNWMERKKLLGKLVEKFDSKIFMPDDYLDKMIDVEARVGISQLK
                     KIDRIIQDRVDAVNYYFDSLKDIKEIQLPPKIEGATYSHFTIQVENRDKLISYMLDKA
                     IQLGQLIEYSVPELDSYKNRNYRVRPECNVASNFVPKVVNLPISSGMKDVQKVVSELR
                     KYYGI"
     gene            25960..>27680
                     /locus_tag="PLF66_15940"
     CDS             25960..>27680
                     /locus_tag="PLF66_15940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65585.1"
                     /translation="MSIYNPNIITLYLKLFYLTAIYCLTGYLVIGKFLKKSIFFSLAF
                     FIFFGVCIQFLLGFLLVELNIKTKYLPLLLLPLFFYRFFLFYKNKKVNLESIEILKNR
                     SSIREVTIFFICLLLSSSFYFIPFISEKTSGFYAYGGGDQTSYFGVSEFVYDTTLKEN
                     FYHLSKYSNQYNFLYETNFPVKSLSYYLFLKANWVLAPQFIAVSPISFAPTYPEESYS
                     AFIAILLFSLNSFIVFLLSKFLIRVSKKKYLIFFFLTMIASGGLSLAWKHATPALFSW
                     ISSLSILVILLLSKEYHIEFLNPFIIAILTLVSFLLYLPSLVLVFIPSLYLFIILLQK
                     GKIAFAFTIQTGIIFLLLGNLNLQYPVRLSLGNAFEDRMLLDYGLKYEQCIKALTGIY
                     DFESLLSFPIKNSRSFEILIVSIVFIGFVIHIFWGKSKRNIAVLLTIVLSLSLSVYYY
                     LKNGHYHVIRLIEFCSILVFPLSAFTLVNFISNKRNTIFLLIRNYFFIFLISVLVFGV
                     IKNRLFVFNLFKNPDYNARASLKNSNSLLLTNDLKKMNTHSEKYFFWMHHGSVQSANN
                     QIMFRNV"
BASE COUNT         9575 a         3879 c         4674 g         9552 t
ORIGIN      
        1 atgctctcca tctgcactgt aaaggaagag tataagccgc aattacctgc aatcactcat
       61 gtggatggaa ccgcaagagt gcagttagtg aatcagaaga cgaatccaag attttataaa
      121 ttgctgaaaa tgtttggaga aaagtcaggg attccgatct tactcaatac aagttttaat
      181 ttaaaaggcg agcctatcgt tgaaacaccg ttaaacgcaa ttaaaacgtt tgagtggtgt
      241 aatatggatg cccttgtctt agaaaattat ataattaaga agtgaaagta atgaataaat
      301 acgaaataga aaaaatactt tttattctct gttctgaaaa cattgaaatt cctcatatac
      361 aaagagtagt taagaatatt agatacaggt ttccaaattt taaagaatct gtatttacta
      421 cagatgataa attctttgat cattacagta aaaacaaaga acttaattta gttaaatcta
      481 gcttgaatgc atacaatgag tttaaagttg tagttttaat tcttgggaat aataatgaaa
      541 gaattctgca agaaaagata gattataata ggacaagagt atatgaagca gactctttag
      601 ggatgattcg atatacaaaa ttagaattgg atgatatcga atatcctttt gaaaacagac
      661 atattttcaa tcaaatcgcc tctaatttag gaaatgagag aattgcttat tttccctatt
      721 cctatataac tcagtatgtg gggcttggtc ctatcaatca gtttggtcat agagttgatc
      781 caaaggaaat caatgcaatt cagaatagag aaaaagatac taaattgatt gttattactg
      841 gtggttctgc ggcttggagt attcatacaa attacgacga gatgtttgcg aattgcttag
      901 agaaaaaatt aaactccgaa aatttaaaat ataaatttaa agtattaaac tttgctcagc
      961 catccgctgt gatcttaaat aatatatttg attatattct tcatgtccaa agattaaaac
     1021 ctgatttagt gattttccat tctggagcaa atgatttagc atttggtcag acttctgatg
     1081 agttgttagt aggtgagcat tcaattattt atcaatttca atttgaagat tgggctaaaa
     1141 ttttagagac agatagaaat tataatccga agaatccaaa aattactttt aaagagatta
     1201 aaaattatcc aagaacaatc attggcgcat tgaaagaaag aattcttcag tttaaaacta
     1261 tcgttgagtc aactggtgga aaatttcttt ttggattgca accgatgagt ttcagtaaac
     1321 aagcaatttc aaccaaagag aaagagagtt taaaaaaata ttctgagaat ttctttaagc
     1381 ctatcatgga taatttaaac tttttattta ataaatgcga atattattta acaaatgata
     1441 aatcgattga actaatcaac tttcataagg aatttaatca atacaacgaa agttatacct
     1501 tgtttacaga tcaagttcac acaactccgg atggagattc gatcattgca gatatttatt
     1561 ttgaacagat aaaaagatat tggagttaaa aggatttaat ttatgagttt tattcgaatt
     1621 ttaatacagt tgatttttag aggattcttt tttgattttt tagttatgag ttatttctat
     1681 atttttaaga aagatcaatt tatttctaat aaagaagtag aagcaaatcc atttatttat
     1741 atgatgcaat aagatcctca tagatccatt tattttactt ttgtatttta ttaagaatga
     1801 gtgcatcatt tatacgaatc tttttagctg agtgctttac tggagaaaat tgaaatgaat
     1861 aattttttat caaagtttat tagagataat ataaatgagc aattttttat ttttgatatt
     1921 ggagcttaca aaggagaatt tgcagaacag tttgttgaaa aaaatatcaa tagtataatt
     1981 tactcctttg aacccgataa aaatagctat acagcgttaa gtgaaaaata caaaaagaat
     2041 tcttttattt ataaccttgc aatctctaat tacgaaggac agaaaaaatt ttatcagcaa
     2101 aagaaggggt atcgctcttc gttactgaaa gataattata gtgaagaaga tacggagatc
     2161 attgatgtaa aggttactac ctttgatcat tttatgaatc aagaaaaaat agaaatcaga
     2221 gaaaattcaa aaattatttt caagacagat acgcaaggaa atgatttgaa tgtattaaaa
     2281 ggtgccgaat cttttcttaa aaaatacaaa ccgatcatta taacggaagt aatttttcag
     2341 cccctttatg attcgcaatg tcattatctg gatattttga attttctttt tagtataggc
     2401 tatgagctag tttacatcga cgggacgcat ctaaatacag atgaaaagat ttcatattca
     2461 gattattatt tccaaattaa agaagattct aaatcagata aagctaattc tagttttcat
     2521 ttacatatag aagaatccgt aaacaaagaa ctagaaatgt ataaaactgc tgcaaaagag
     2581 agattagatt taattcaaag attagatcaa gaatgcaatg atcgtttaaa agtaattgaa
     2641 tggcagcaaa aagaaataga aaaattaaaa agtaaatagg tggtagtatg ggtgcagtaa
     2701 atttctcact tgataaaaat ctagtaaaaa caattcaaaa cttttctcca gtcgatactt
     2761 tagttgaaac aggaacattt gaaggagata cgattcgaaa tctactcaat gattttaaaa
     2821 ctatttattc gacagaaata tcaattgaat attttgatcg agcaatatcc gattttaaaa
     2881 atcataaaca tgtaaaacta tatcaattat catcccctga atttataaaa aaaattcaac
     2941 cagatataaa gaataattct agtatatatt ggttggatgc tcattggatg gtagctgatt
     3001 ttgcggcagg cgaaaagtct cagtgtcctt tattagaaga gttagacgcc atcggagaat
     3061 taaacaacga tagctacatt tttattgatg acgcgagact ttttctaaca actcctccgt
     3121 atcctcatga aaaatctcat tggccaagct ttgattccat tgtgaataaa ctgcaaagtt
     3181 taagtaaaaa acataaaata atggtattaa atgatacaat cttgttttat ccaaataaat
     3241 cagaagagat catcaatgaa tatgcttata aacactccat tgattggcta gctattttac
     3301 ataaatcaag agattatgat aatttaagag tccagtttga tggattaatg ccacaaatgg
     3361 aaaaattaga agcggaaata ttacaatcaa aacaaaagga tgtattgatt aaaagtttac
     3421 atcggtttgc aaatgaacta caacaagaat taaaaagaaa acaaattcca tttgagactc
     3481 aatttgcaat gttaccgggt aatcttttgt ctatcattat cccaacttac aaccaagggc
     3541 aatacattga agattgttta aaatcgattt taaatcaaac ctaccaagat atagaaatca
     3601 ttattcaaga ttcaaattca acagaccaaa ccgaatcgat ttgtaaaaaa tacgcaacga
     3661 tagatccaag gattcaatac tatagagaaa aagatgaagg tcaatcggat gcgatcaata
     3721 gaggactcgc aaaggcaaaa ggggactact ggacttggat atgttcggat gatcgctacc
     3781 atgcaaatcc agatgcatta aaaaatttaa tccaacactt ctttgaaaag catagtgaaa
     3841 gttcaaaggt agtaggtgta tatggcaatg cgttttttat gaatgaaacg ggtgaaattt
     3901 tacatgagta tggaaatttc aaaaaagacc taacggtaga acacttcatt cacaattggc
     3961 ctctttcaca accatcttcc atattgctca caaaagcagt tagacaagtc aatggagtcg
     4021 ataagaattt atttttagga atggatttag acttatttat taaaatttta aaaaatggaa
     4081 atcagtttgt gtttgttgat actcctgttg tggatatacg tttgcaggac aattcgaaga
     4141 gtgtgaagta tagaaaggat actgcattaa atgcgttgaa tttgatttta tcacatttta
     4201 atacgattgg caattatcag gaatccgctt atttcaaaga actatttctt acggcagaaa
     4261 caactgagat tcgaaaattt ctatcaaaac taaattggaa attcaaatgg aagtatagag
     4321 tcatgaatct attagaaaaa acttccatca cacaaaagct tttacattta ttaagaaaat
     4381 taaggggata aaatggcaaa gaattttaca gttattggaa taggtggcta tatagcaccg
     4441 agacatttaa aggcaatcaa ggaaacaggc aatcatttga tttcttccta tgatcgaaat
     4501 gattcagttg ggattgtaga ttcacatttt ccagatgcag atttttttac tgaatttgaa
     4561 agatatgaaa gttttaatga gaagcaaatc agagagggaa ataaaattga ttatgtaagt
     4621 atcatgaccc caaattattt gcactttcct catatgaatt atgctttaaa aattggtgca
     4681 gatgcaatct gtgaaaaacc gttagtctta aatccaaatg aaatagatac tctaatcgaa
     4741 ttagagaagg tttacaataa aaaggtatat actattttac agttacgtca ccatcagatt
     4801 atcatcgatt taaaaaataa aattgataat cgcaaatctg atagtaaaat tgatattgat
     4861 ctaacgtata taacttctag gggaaaatgg tattttgaat cttggaaagg ggatgtaaga
     4921 aaatcaggtg gacttgcaac caacatcgga attcattttt ttgatatgct gacttggatt
     4981 tttggaaaac tagaatccaa cgaagttcat ttaaatgaac ctgctaaatg tgcaggctat
     5041 atggaattag aaaaagcaag agtgaaatgg tttttatcag tagactacaa tgatgttcca
     5101 gaatcacaaa agaaaaaagg acaaagaact tacagatcca tcttaatcga caacgaagaa
     5161 cttgaatttt cagaaggatt tacagattta cacactgtaa gttataaaga aattttagaa
     5221 ggtagaggat atgggttaaa agatgcgaga gagtctatcg tcattgcaag tcagattcga
     5281 aatcttccaa ttaccaaaac ttctaataaa cataggtttg taaaataatg agtgattttt
     5341 ttattcatga aactagttta aaagatgaat ccgcagtgat cggggaaaat actaaaattt
     5401 ggcatttttc ccatatttta aaaaatactg tgatcggaaa gaattgttct tttggtcaga
     5461 attgcgtagt aggaccaaat gtaaaagttg gaaacggagt taaggttcaa aataatattt
     5521 ccatttatga gggcgtagaa gtagaagata acgtttttct aggtccgtct atggttttta
     5581 caaatgtaat caacccgaga agctttatag tccgaaaaac agaatttaaa aaaacactct
     5641 taaaaaaagg ctgtagcatt ggagcgaatg caactatcgt ctgtggagta acaatcggta
     5701 gatttgcact cattggtgct ggctctgttg taacaaagga tgttcctgat tttggaatgt
     5761 tttatggaaa tccaggacga ttacagggat ttgtaagtat agctgggaat cgtttgaact
     5821 ttacaaataa tagagcggtt gattcctacg atggaacaga gtatgagtta agagatggtc
     5881 tatgttatgt agtcaatgca ggtagttatg aaacttaatt ttaatgatct aaaaacacaa
     5941 tacaatcgaa tcaaagtaga tttagataaa tccatacaaa atgttttgga aaagcaaaac
     6001 tttattatgg gagaggagat tgagatttta gaaaaaaaac tatctcaatt tacaggcgca
     6061 aagtattcta ttagttgtgc caatggaact gacgctttac agattgctct gatggcgata
     6121 gatattaagc cagacgatga ggtaattact acaccatttt cctttatatc gactgtagag
     6181 gtgattgcat tactcggagc aaagcctgtt ttctgcgaga tcgatgagaa aacatataat
     6241 atagatgcaa cgaaactaga aagtttgatt acaaagaaaa caaaagcaat cattccggtt
     6301 agtttatttg gacaagcatc tgatatggat acaatcaatc aaattgcaga aaaatacaat
     6361 ttaacagtga ttgaagatgc cgcacaaagt tttggtgcta gatataaaaa tataagaagt
     6421 tgtaatcttt caaaaattgc ttgcactagt ttttttcctt ctaaaccact cggttgttat
     6481 ggagatggag gagctatttt tacatcaaac gatgaactgt atgcaaagat aaaatcgatt
     6541 cgaacccatg gacaagaaaa aaaatattat taccagtatg tgggagtcaa ttctagattg
     6601 gatactttgc aagcagcagt gctcaatgaa aaaatgaaac tatttccaga agaatgtgag
     6661 ttgcgcgaga aagtagggag tcgttatacg gaacttctca agactaaaaa aaatctaatt
     6721 acgccattta tctcaaaaga taaccattcc gtttatgcac agtattgttt aagagcagaa
     6781 aacagagagg agattatgaa tcatttaaaa acaaatgaaa ttcctacaat gatttattat
     6841 ccacatcctc ttcatttgca aaaagcattt tcttatttgg gatacaaaac aggagatttt
     6901 ccaattacag aaaaaatttc gaaagaaatt tttagtattc cgatgagtcc ctatttaaca
     6961 gaaaaagagc aggacttagt tgtaagttat ttgtgaaaga aaaaagaaaa cggtcttatc
     7021 ttttaattta aactcttaaa agttaaaatc taagaccgaa tgaaaaacca atcaactcaa
     7081 agatctctga gttcggttcc tcccttaatc aaaaaaaata caggaaataa aatgaaaaaa
     7141 attctaacca ttgttggggc aagacctcaa ttcgtaaaag cagctgctgt cagcagagaa
     7201 tttaaaaaac acaaagatat acaagaagtg attgtacata caggacaaca ttttgataaa
     7261 aacatgtcgg atattttttt tgaagaaatg gaaattccaa aaccggatta caatttgaat
     7321 atcaatggtc ttgggcatgg ggcaatgact ggacaaatga tggaaaaaat tgaagaagtg
     7381 attttaaaag aaaaacctga tttccttatg gtctacggag ataccaattc aacactagca
     7441 ggcgcacttg ctgctaaaaa gatacatgta agagtgattc atatagaagc aggacttcgt
     7501 agttttaata tgaacatgcc agaagaaatc aatcgtattc ttacagatag aatcagtgat
     7561 atcctttttt gtccaacaga ggtatcgatt gaaaatctaa aaagagaagg ctttgaaaac
     7621 ttcaaagttc aaatagtaaa gaatggagat gtaatgcaag atgcggcaat ttactattct
     7681 aaaattagtt ctacaaaatc tcgtattata gaaacactag ggttgaagga tcaaaaattt
     7741 atcctgagta caattcatag agcagaaaat acagatgatt tagaaaaatt aaaatctatt
     7801 ttgtctgcat tgaatcaaat caataaagag attacgatcg tctgtccttt acacccaaga
     7861 actcataata ttataaaaaa aaacaatcta tccacagaaa taaaaattat agaaccagtg
     7921 ggatactttg atatgattga gcttttgaaa aattctgttc ttgttatgac cgatagtgga
     7981 ggagttcaaa aagaagcttt cttttttgag aaaccttgta ttacgatgag agaagaaacg
     8041 gaatggttag aactagtaga gagcggatat aatgttcttg tcggaagtga ttcaaagaaa
     8101 atcattgaat cagttgaatc gatgaagtca aaaaaattag attttagtaa agacctttat
     8161 ggcaaaggga aaagttgttc tataattgct gaaactatat tgaattataa aaattaaact
     8221 aaaagagtgt tacaatgaaa gaagagttaa ttaaaaaact gaacaataag tctgctatca
     8281 tcggaatagt aggattgggg tatgtgggat taccacttgc tttgcgatat tccgaagagg
     8341 gttataaagt tttaggtata gatattgatg agttcaaagt aaatgaagtg aactttggta
     8401 gaagttatat agaacatatt acatcagagt ctattcaaaa ggcaaataaa gttggtttta
     8461 gtgctacaac tgattttcaa agagccggag aagtagatac actgattatc tgtgttccaa
     8521 ccccactcaa taaatatcga gaaccagatc taagttttgt aacagataca gtagatgctt
     8581 tattgccatt tatacgtgca aatcaagtga tgtcattgga aagtactacc tatccaggaa
     8641 cgacagacga agagttaaaa cctagaattg aaagcaaagg cttcatcgtt ggtgttgatt
     8701 attatttggt ttattctcca gaaagagaag atccaggtcg taaagatttt aataccaaaa
     8761 ctactcctaa agttgtaggt gggtttagtc ctgattgtat ggaagttggg aaagctctct
     8821 atggtggagt gattgataaa gttgttcctg taagttctac aagagcagcg gaaatgacga
     8881 aactcttaga aaacattcat cgctctgtaa acattggtct catgaatgaa atgaaaatcg
     8941 tagcagataa gatgggaatt gatatttttg aagtgattaa tgcagctgca accaaaccat
     9001 ttggttttgt tgcctatcat ccaggtcctg gaattggagg acattgtatt ccaatcgatc
     9061 cattttattt aacttggaaa gcaagagaat atggtgtgca cactcgattt attgagttag
     9121 caggcgagat caatagctct atgccaaatt acgtggtaga taaaacgata gaagctttaa
     9181 ataaacatgg taaggcttta aaaggggcaa agattttagc tttaggtatt gcttacaaaa
     9241 agaacgtaga cgatatgcga gagagccctt ctgtttttgt aatggaaatt ttaagagcga
     9301 aaggcgcgat cgtagaatac catgattccc atgttccagt gtttccaaaa atgagagaac
     9361 accattttga gttatctagt gttccgctca cagttgaaaa tttaaaacaa tttgatgcag
     9421 cgattgttct cacagaccat gatgatgtag attatgattt agttcaaaag aatctaaaga
     9481 ttgttgtgga tacaagagga cgttataagg gttctttcga caatctagta aaagcttagt
     9541 agtaaaatcc tctcttttcg aaaaacgatt agcttagaag agtggtgaaa agaccattct
     9601 ctaatgctaa tccgttttta tactctaaat cctaaatctt cttttaaaca gaactaggtg
     9661 caaactttgg actttcaaaa atcagataga aaaaatctaa ttcttttagc agtatggtct
     9721 ctctttttgt tttttattgc taaaacattc cgtgcttatt actctatgga tattcctcct
     9781 gcttgggata acttagccta tcagctagaa ggagtaaaac tggctcgctc tttttttgag
     9841 ggtgatagca gtgtgattcg aagttatttc ggagataatg ttccagtagg ttatattttt
     9901 ttactatctg taagttattc tgtatttgga tttttaccag agtctcctta tattgtctcc
     9961 gctctttttg gatttgggaa tctatgtttt atttatttaa tctcaattga attaaacgtt
    10021 aagcgaattt atgccctctt agccagccta tttatttcaa ccttaccgaa ttttatttat
    10081 aataattttc tacagactcg aaatgatttt gctcttagtt tttttatttt attttttctc
    10141 tatctactca tcatttcttt aaaacagaaa aatgtttggt tggtgtttat tgcgggaatc
    10201 gtttctggga taggcactct ttttaaattg agtgcacctg gatattttat ttggatgtat
    10261 cttttgtttc tattgattcc aaatgatcgc aatgacttta tgactcgtct caaatgtttt
    10321 ttgatcgcag ggttaggggc aatagtcact tgtggttggt attatatacc tagtctaaaa
    10381 aaaatactcg cttactattc tatgtgggga agtttttccc aaactcagta taaactattc
    10441 agtttttccg atcgattctt attttatttt aaaaatttta tttctactca tattggatcg
    10501 aattcaatcg caattatttt actaagtatt ttaattgtag ggatcctctt aaatctgatc
    10561 atcaagtcct accgtgataa acaaagagaa ttaaaagaat cgtttcaatc ttcttatctt
    10621 tatttgattg tgggtagttt tttctctgtt attcttttcc taaccttgaa tcgatctttt
    10681 tcggaagcag gtgatacacc ggttctatcc attttattta tattgatttt agtgattcta
    10741 aacaatttat attcacaaat acgagtccga aaaatgattt taatcctatt tcttgtattg
    10801 ccaatttctc tcggaaattc attttccaaa atcgcaaaag aaaagctcta ttccatctca
    10861 gaatttgaaa cctttgtgaa tcaaatccag gagtttcgaa aaaaatacgg aatggaagaa
    10921 gaaaaatttt tacaagtttt ctcccatcct gtttacaata caaatgctgt tgcttggtat
    10981 ttgcatttaa cgaatcgagt gccactgaat tcactcataa aagcaagtat agagattgcg
    11041 gattggcaat ctctctctgt aagccatgat gtggcagaga tggttcgtgt tgtaaataaa
    11101 tatcctttta tcattgtttc aaatgaaaat cctatttctt ttggtggaga gtattttgtg
    11161 caagtcaatc tactcaattc tgaaatcaaa agacaaatct ttgaatctgg aaattacatg
    11221 tgtgaggatt catttgaaat aggaaaatca aaactcccga ttcgtttttg tatcaataaa
    11281 acctatagac aatttaaatt tttaaacaaa acgactgacg gttggttaga atgggatgca
    11341 catgcgagtt actattctag tgttagtgct aagtttttaa tcaaaggaat tccatctaga
    11401 gatataaaag cattttattt agaagatatt agaacaaagg aaaagtttaa tgcaaaattt
    11461 atatcggttg tgaatcattc agaataccaa tatgaaattc tactccctcc ctccacatct
    11521 gtcagaacat tcaaattact agcaagcaat gagcaaatgg tgcctgcttc caaagaagat
    11581 atccgtaaat tagctcttct aaatacaaaa attgagtttg tagaataaat gagaaaatta
    11641 aaattaattt ccacttccct tacgattcta attcttgttt gctatatagc aatccttgca
    11701 gtctaccaaa agagtttaga taaaaacgca attccttggc atgatggctc tcatcatttt
    11761 actttggcag ctaatttatt taatcaccta agctcgggtg attttgaata ttatctagaa
    11821 tcacttcgaa attttccgga actgatttta acttattcca ttctaaacct tgcttttctt
    11881 ttagtggggg gagtcaaaca ggctgctata ccatttgtct ataccttatt ttttattctc
    11941 atagtcttgt cgtattttct ttcgatttct aaaagagaaa gacatacgaa atccttatta
    12001 tttacaattg tatttatttt atctggaatt tatactacgc aggcaggagg agcgttagac
    12061 acacgagtag atatattgtc tgctagtgct ggagctatct cacttttatt tataacgagt
    12121 cgaaattttt attttggtat tttatttttt ctatttgcct cctttttaaa aggagctgcc
    12181 ttttttctga ttttaccaat gcttttgatt gggattgcaa ttgaatattt aaaagaaaaa
    12241 atcacacttg attggaatct caaattccta ttgaaaagta tttccattgc aataccactt
    12301 ttgatttttc ttaaagtact tctcgcctcg gttttatctt acaatttaat gcagacagga
    12361 ggcagtaatt tagaaagtag agttcaaatc tttatatcca atgtgaatca atatttaatt
    12421 ggaggatttg gttattacta caaggtatta aaagaaaata caaatgtagt ttttattttt
    12481 cttggaatca tcgtagcagt tctatactca atttggcaaa agaataaaaa attaatttta
    12541 tccggatttt ttgtattttt ctgtatttcg tattcttatg tactcatgac aatgagtcca
    12601 cttaaatcag gagtgcttat catctggttt ttaccttgca caattgttgc ttccatcttt
    12661 tttacagatt ttatttttaa aattagatac aataaatacc taatccttgc cctattgtcc
    12721 ataacgttta ttgtattata taaaatccct cgaaagtata acataccaga agattatttg
    12781 cagttggctc gaaacaatat acaccagcaa gcaagtgaaa tcgcaattca gttagacgag
    12841 aaatatcctt ctcgattcgt aaaaggagtt gtgtttgtaa attttctttc tgctcatgga
    12901 gttatatcct ataattatga ttcttatacg aatttaattc ataaaaaggt gaagaaggca
    12961 aagattgacc tagcaggttg ggaacttgct acctattcaa aggaatggaa aaaagaaata
    13021 gaacgtttca aagactatga tctgattcta ttgatcttac aagaagaacc aatgggaatt
    13081 cctgccacaa atacacctca gaaatatggt agagaagttt atttagaact agaaaaaagt
    13141 agaacagaaa aaccgagttg ttttacaaag attacaaata caattgattt gcctcatatt
    13201 gggaaaagag atacgtattt attttctaac gttcccgatt gtaaagcgat tttatttcca
    13261 tagcatacat gattttttta attcaataca ttctatgttt ctggggaatc tattatattt
    13321 tcagtggggc aggggaagtt ttaaaaaaat ttctgaaact aaaaggctct ccgtttattt
    13381 ggattcagaa aatagaaacc attttatatg ggattggttt tgttcatttt acaagtacca
    13441 ttttacaact ttatttttct cattctgtga gtagtatagt ttatctttta tttcttattg
    13501 ttctaatgtt tttaggaatt tacttttcga tcattcaatc caaaacaaaa atacagtttg
    13561 caaaactttc ttttgttcat tttcttcttt ttcttttttt gcttttacca tttagaaatt
    13621 tttcctctga atttatggct tctgaggagt ctggtgatca cacaatttac ctaggtcctg
    13681 taaaggcaca gtatatacaa aacgattcag aaattacaaa gtcaaaatgg aagttggctt
    13741 tgactagagc tgtctatgat tctagaattc atttgctctt tccacctata gaaaaaatat
    13801 ttcccatgtt tgaagccgct aaaaaagcaa aatgggaaat cgatccaatt gatcgagtga
    13861 tcttagaagg attttctcct gcttggtggg cactgcattg gggtttgatt gggtttggat
    13921 ttttgcttcc gattcatccc gaaaccattt atttgtctat tttgtttttt ttaacttctc
    13981 tcctctgttt acaagttggg tcgataggtt atttgctttt caatgagtat accgaatcta
    14041 aaatcctagt cttctttttg attcttttgc tttctctttc tgcttgtatt gcaaaaatca
    14101 catatggaca tttttattta caattgattg gacttgtttc cctgctttgg atttttaaag
    14161 caactctttc ttgggagaaa acaagagata aaaaaacatt ctattctgct cttgtatttt
    14221 catttctaat tggaccttcc ttttatttta tgggaactgt ttactggggc ttgattgtac
    14281 tttgtttttt tctcttttat tataaaacaa atttgctcat tggcttattt cattatatcc
    14341 gtaagccaag tattttgatt ggatcttttt tttatttacc tatcttatta gtcaacattg
    14401 gttctttttt aaaatttcta ccttccacct ctagttcgat gcaaaacaaa caatattggg
    14461 atcaggtttt aggtgggaca gtcacaggct ctgatgcttt tttaaatcca attcttggaa
    14521 tcgttactta ctttcagttt tatcctaaac ttccttttaa agaacaaata gatgctctca
    14581 attttatatt ggttcccttt gcgattggaa ttctttttct cttaattggt ggaattttca
    14641 aatctatatt ttttgatttg aaaaaaaatg taagaaagaa tttgttttta atttttgttt
    14701 taattggaat tcttactatc aatctgaaat ctgccactca ttattataat tacaataaag
    14761 catctcagta tacatttcca tttttgattt tatcttttcc ttatttttat ttgaaattaa
    14821 atgcaaatcg aaagtctttt tatctacttt ctcttatttt aattttctgg attctattgt
    14881 tgattcctgc taaatttaga cagattgaat ccttttcctc tttgcatgaa aaatcaatga
    14941 gtaattattt actcttaaaa gagcgactag atcacctaaa taaaaataca atctttttct
    15001 ttggagaaag cagaaatcat gcgttattgg aaacatatat ggctggaaat caaacaattc
    15061 cagttggaat ctacccgatt gatagtttta aaaaacattt tcattttgaa aacctctctc
    15121 taaacaaatt tatgttaaaa gataaggatt cagagtctta tactcttttt gatctctccc
    15181 gttttttgat tggagctgat gagtattggg ttgctcttag ctcttcgatt cgacttgatt
    15241 caacaaaaat accaaattcc ctttatttga attctattgg gaaggtagaa gtatttgcag
    15301 aattaaaatc actcaaaaaa ttgattgtct atacatttca ttcgaatact aaacctacag
    15361 ttaatagaga gagatttaat tcgatcgaaa ataattatat gcaacctgaa aatttgcaat
    15421 caatcgaaac ggtttgtgca aagaacgagt gtaagtatac gtatcatctt gaaccactgg
    15481 gaaaaaactt ttttttaatc gaaaatgctt ctagggtaga agttttttga tgtcttttgt
    15541 agtactgaaa taaatattaa ataataagga attgatatga agaagaaaat tatgattaca
    15601 ggtgtagccg gatttatagg ttccaatgta tgcaagcatc tattacaaaa aggttatagt
    15661 gttgttggaa tcgacaattt atcccagagc gatggaaaaa acatgaatga attcaaagaa
    15721 aattcaaatt ttgaatttta tagagttgat atcatgaatg aaagttcaat agtcaatgct
    15781 gctcgaggct gcacaggaat cattcatcta gctgcttaca agatccctag atattctgat
    15841 gcgttagata cattgaaaat caatgggatc ggttctgaaa acctgatcaa agcggctatt
    15901 gaaaataacg ctcggcttgt tgctgcttcc acatcggatg tatatggaat gaatccagag
    15961 cttccttttg cagaggataa atccaaatta gtaatgggtg gtccaaaagt tagaagatgg
    16021 gcatatgcaa tttcaaaaat gcttgaagaa caaatgattt ttgcttatgg agaaagatct
    16081 aatttagatt tttccatcat tcgttttttt ggtggttatg gtccaaatca aaatcttact
    16141 tggtggggtg gtccacagtc tgtatttatc aataaagcgt tagacgatga ggagattgaa
    16201 gttcatggag atggacaaca aacaagaagt tttacctttg ttggagatca tgtggatgga
    16261 gtcactcgtt gtttagaagt agaaaaagca agaggggaag tttttaacat tggaaacgat
    16321 agagaaatca ccattgaaaa tcttgcgatt atgatttgga atttaattcg accagggact
    16381 acaccaaaaa tcaaaaaaat tccctatgag acatttggaa agtatgaaga tgtaatgaga
    16441 agaattccag atctaaataa atcaaaatcc attttaggat ttcaacctaa ggttcctctg
    16501 gaagaaggtt taattcctac aatcgaatgg caagtaaaaa gaagaagaga attaggaatg
    16561 ccagaaccaa aatcaggctc ggtatttaaa taatgatgaa taaaaaaatt tttattatta
    16621 ctcctgtatt aaatgaagag ccaaacatgg aaagacttct ttctggatgg aaaagcattc
    16681 aaaaagattt aaaggatttc gactttgagt ttatactcat tgatgatggt agcacagata
    16741 atacagtctc aaaagctaag tctgtaaaag aaaatttaaa tcttaccatt ctctctcacg
    16801 agaaaaacca aggtcccgga tatgcttttg gaactggctt tgaatatcta gccggtaaat
    16861 tgcaaccaca tgatctagta gtgacaatgg aaggagacaa tacctctaga attgacacct
    16921 ttaccaagct agtctatcga cagctgcgag agggggatga tgttgtttta gcttctccct
    16981 atgcgtatgg tggtggaatg accaatacag cttggtatcg aattatctta agtcatatcg
    17041 caaacggtat ggtcaaagag ctcattggga ttcatggaat ccatactatg agttcctttt
    17101 ttagagttca tacaggcgaa atcatactgc gtcttcaatc taaatttgga aagcgaatta
    17161 tcgaaagaag aggatttgaa tccatgattg aaatgttaaa gaagttaatc ttagtaaatg
    17221 cttctatgtc tgaagttgct atgaaattgg atacatctct ccgagcaggt aagagcaaaa
    17281 tgaagattat gcgcacaatt cgtggttatt ttaaattgtt tatgatttct agaaaatgga
    17341 aagtttagga gtataggaaa tgaaaatatg gattttatta cctgcattta atgaagagga
    17401 gtctcttcct agactattac ccaagatttc aaagttctgt ctagattcaa attttaatta
    17461 cgagattgta gttgtaaatg atggtagttt ggattctacc ctatcaattt tacaagatta
    17521 ttcaaaaact cttcctgtta caattgtaaa tcatgtgatt aatagaggtc ttggagaaac
    17581 agaaagagac gggtttgagt atatcgcttc taaaattcag gaagaggata tattgattcg
    17641 aatggactgt gatgataccc atgaaccaat gtatatgcct agtcatgttg aaaaaatgag
    17701 agaaggattt gatgttgtga tttcttctcg gtttcagcca ggcggtggtc aattaggggt
    17761 aaatggatat agagcgttta tcagtgcttg tgcaaatata tttatgaagg ttctatttcc
    17821 aatcgaaggg gtaaaagatt tttcttgtgg atacagatct tataaaggta aaatcattaa
    17881 ggatgcgatt aaggtttttg gaaatggctt tatccaatta aaaggattag gctttacttc
    17941 aacccttgag actatcgtta agttaaaact tttaggtgca agattttcag aagttccttt
    18001 tgtattgaga tatgatcaaa aagtaagttc aagtaaaatg ataagtagta ttactacttt
    18061 aggatattta actatggcta ttttatatca ttggcctttt tccggatggc gaaatagcta
    18121 taagggtcta gcaaaaatgt atagagagaa ccctgaacaa gcgatcctaa aatacaatcg
    18181 atttgcgata aaacgtggat taacgactag aattggcggg ggttgagtat gtgtggcatt
    18241 gtaggttttt tttccaataa cctgaccaaa gaaaatcaaa tcataaattc aatgctcacc
    18301 tctattcagc atagaggacc tgatggacac ggagtatttg atgttgataa tattgtttta
    18361 ggccatgtta ggctagctat tatcgatatt cagaatggta tacagccatt cataacagca
    18421 gataatagat atgttttaat ttttaatgga gaaatttata attatttaga attaagacaa
    18481 acattagtct ctaaaggtta taagttaaca acttattcag acactgaagt tttgctttat
    18541 atgtttgtag agtatggtaa aaaatgttta gattatctaa atggaatgtt tgcctttgca
    18601 atctatgata gacaatccaa agaattattt cttgctagag atcattttgg aattaagcca
    18661 ctttactatt atgcaaagaa tggaaaattt gcatttgcat ccgaaatcaa agctttactc
    18721 aaacatccag aaattcaggc agatcttgat gaaaactctt tatatgaata tctaaccttt
    18781 caaatggttt tagataagaa tacactcttc aaagatatct ataaattaaa tcctgcctct
    18841 tatatgatag tcaaggattc aaaaattttg gaagaaaaag tgtattggaa tttagattac
    18901 acaattgatg aatcaaaaac tatagaccaa ttttctgatg aactactcat attacttgat
    18961 agttcgttat ctattcaaat gaggtctgat gttccagtgg gtgcatatct cagtggaggg
    19021 ttagattcaa gtattgtatc tgtacttgct gctaagaatt atcttggaga atttaaaact
    19081 tttacaggtg gattcagaga atctagtgat tatgatgaaa cttactatgc aaaggttgtt
    19141 agcaataaga tcaaaagtca gcattttgaa atttttccag accataatga ttttcttaat
    19201 gtatatgaaa aactcattta tcatatggac gagcctgcag caggtcctgg attatttcct
    19261 caatacattg tctcaaagct agcatctgaa aatgtaaaag tagttttggg tgggcaaggg
    19321 ggagatgagc tatttggtgg ttatgcaaga tactcagttg cttatttaga gcaatgcatt
    19381 aaaggggcaa tttttgattc ccaagaagag gggaatcata tagtaacatt gcagtccatc
    19441 attccaaata tgcctcttct aaagcaatat gtgccaatga tgaaaacaca attttctgag
    19501 ggattgtttg atgaaatgga caagagatat tttcgattgg tggatcgttc tcctagttta
    19561 aataaaatat attcatccga tttcttaaat caaatcaata aagaaaaaat tttcgaaaag
    19621 tttcaatcca tttttaattt cccacaaaca aagtcttatt ttaataagat gacccatttt
    19681 gatacgaaga cattgttacc atctctatta caagtggaag atagagttag tatggcggtt
    19741 tccattgaat caagagttcc acttttagat aaaaggattg ctgaactctc agccaaaatg
    19801 ccccctacaa tgaagttctc aggtggaaaa acaaaatata tgttattaaa tgccgcgaaa
    19861 aatatactcc cgactgaaat agtaaatcga aaagataaga tgggatttcc tactccaaca
    19921 aatgagtggt ttaacggtcc gttaaaaaat tatgttttag atcatctact gggatcaaac
    19981 tcaaaaacaa aacaatattt taattctgcc aacctagaaa aagttttgaa aggaacagaa
    20041 aaatttagca gagatgtttg gggtgctttg agtttagaaa tctggtttaa aaccttctta
    20101 tgattctata catccgaacc gatttaagtg acacatcttg gttagaatat attctttcag
    20161 agtttatcac gattcaaaat gcaaaatttg aatttcaaat tgttcccctt ggaaccacta
    20221 ccgatgaaaa atctactctt ttttatacta aagaaaaatt agggaaatta aattgggttc
    20281 caaattattc aaatagtatc gtatcagaaa atttaaaata tatcgattct agaatttgta
    20341 ttttagagaa tacgggtgat gaaaaaaatt ttgatatctt ttggaatagt tttgtttttt
    20401 taactcgcta tgaagagtac ctctcagaaa aaaaaggtaa aaaaattcaa agctactcat
    20461 ttaatcatcc tcgaaaagat aaagaaagct tttctctacc cattgtaaac atactttttc
    20521 agtatcttga atcctatata aaggatagat ttaaaacatt aagattcgaa gatacaaagg
    20581 aaatgtatat agaattttca cacgatgtag actacattaa gaaaacgatc caactcagac
    20641 tgaaacaaac aatttttaat attataaaat cattgaaaat acttttaagt ttttctcttt
    20701 tctggattga attaaaaaga ttattctatt ttctattttc aactccatcc tattggtgtt
    20761 ttgactattg ggtgaatctg gaaaaaaaaa ataatattcg atctatattt tatatatatg
    20821 taaagacaaa aacaaatcgt ggttttattt cttggttaat cgatccaagt tatgatattt
    20881 caaacaattt tgaactccaa gaaaagttaa aacatttata tgaggaagga tttgagattg
    20941 gtttgcatgg cagttataaa tcagcactgg ataaagagca gttagaattg gagaaaaata
    21001 ttctagaaga aattttagga ataaagatta caaaaactag acaacactgg ttgcgctttg
    21061 aagaaaaaat tacacctaaa attcatagcg atctttttaa gtttgatgca actttaagct
    21121 ggaacgatag aatgggtttt agatctggaa ttgcaagtca acatagagcc tatgaccatg
    21181 aaaataaaaa gcctttctcg catgtgatta ctccaaatat attaatggat tccaatattt
    21241 atgattacgg gatcaccaaa gtaaatgaat tagaaaaaaa atccttagaa ctactccaaa
    21301 gtttagatca gtataaaaat atatacattg ctctctcttg gcatcaaaga gtttgttctg
    21361 aagattataa atggcatttt atttatgaaa aatttttgaa ttggtatagt aaaaagagat
    21421 agaaaaaatg aaacaaagta aaattagatt ttttatattt acaatattta gcatttgctt
    21481 cgcggttgga tttctttggg tcatcataga ctctattatg gtttggagag gttttaaacc
    21541 gcttgatctt tcttatggtt tcgagacaga atttctcgaa ggaagaggct atgccatgag
    21601 gagggggact caaaattata ggacatttat aaatagttta ggtatgagac gggaagaatt
    21661 acctgaatta gttcctgggg aaaaaagaat tttaacactt ggggattcta cttgttattc
    21721 tagtaacgct agttttgaaa attcatggcc aaatgttctg gagcgagatt taagaagtaa
    21781 gggaaataag attactgtaa tgaatggggg agtcccagga tacagtacta tgaatatgta
    21841 ccatgctttt aaatggtatc taaagaggac aaaaattgat tatgtaatta tacatggcgg
    21901 atggaataat tattggaatg ataaggcact ggtagaaaat tttgatccag attcatattt
    21961 aaaaaaaatt tttccgaatt ctcctgatct cgaggatgtg aataaaaatt taaccgttat
    22021 tgaaaacatc gattctagta gtaatttact tgaacattct tcaggtgcaa tgaaatacat
    22081 cgctactagt tccgctttag gtcattattt agttaaaaat atttggttaa ctatcattga
    22141 cccaatgtta agaggatcaa tttcccaagg atctaaagta aattcagttg acaataaaaa
    22201 atctattaca tcagtgaatc cagagttagc tatcgatgat tatatgttgt atttgggatt
    22261 attaattaag gaagcaagga aaaataatat aactcctatt ataataaagc ctttttcggt
    22321 tttgcgtatt cctggattta atgctttaaa agcgtcaaaa gaatatccat tgtatatggc
    22381 aaaagattat acttggatga taccaaggat gaaggaaatg gataaagcaa gtgaggtatt
    22441 ttcaaaatat aaccttgctt agtatattga tccaactagt gatttactaa aagaaataaa
    22501 tcttgaaaac gtttttcata aagatgtgaa tatttcctac atgggttctc ctagtcaccc
    22561 attcgataga ggtaatattt tgttaagcaa gatattggct gaaaaacttt ctgaacagaa
    22621 aatccttgag cctgcaggaa atgttaagtg ggttcatcca aaggagaaac agtatcttta
    22681 tgatcttaag tttagagaag taaattttaa agttggtaag aaaggattac tttttgtagc
    22741 tctattcagt ttatttttgg ttattagcgg tatatcaata atgagtttct taaatcaaaa
    22801 actatctact attcaattgg ctatttctcc attcatttct tatttggttt tagtttcaac
    22861 tcaatatata ctctttcaat tctcagaaaa gatattaatt aatttttctt tttcttgttt
    22921 catttttata attatgataa ttttctccta cagaaaaaaa attagggaag acataattag
    22981 aatcatttcg cctaaagtgg gattatcaat atttctttct gttctatcta tctgtttctt
    23041 ttctctttat ggatttgaaa aacttaatag tataacatgt tctactttat ataatggttg
    23101 cagattgatt caagaagact tggcgcttac tgatttttta tttgataaag ataaagattc
    23161 ctttcgttta tattctctgt acacgctttt acagtcaggt gaggatagac ctaaattatt
    23221 aattgatata atgtacaatt attctatgtt aaaacaaatg cttccacttt tagagggacc
    23281 tttgtattat ttttcaaata tgaatttaga gactatcaat ttatttttta tttttctacc
    23341 tattgcgttg gtttctaact tagtatttca aatattgttt ttcgaaagat taaccttttt
    23401 agcaaagaca tttgcatttg tttttatttt tactttattt aataatctcc cgttactttt
    23461 taccaatatt tcaaaggtat ggtttttagg aactataggt atattaataa cttcttttgt
    23521 gtcttccgaa aaaatctata gaagtttttt cttatattat caattggttt aagtagtctt
    23581 tatccagata agttgaatgt tattttatta ttagggttac ttattgtttg gtcttttttc
    23641 gagttaaaga aagaaaaaga atttaaaagt tctgatattt ttaacaatat attgttatgt
    23701 atagtgttgt attttatttc tagaatttta ttttttcaat atgtaccttt caattcagaa
    23761 tttttagaaa aaatatttaa agaaagaatt tttgctcatt caaatttaga tctaaatttc
    23821 tttgttttgc ctattttgat ttttacctgg attgctactt tttatatctc aatcaaaaaa
    23881 cttcctattt ggaattggtt gattgtttta attcatattc tttttatgta tatatgttat
    23941 aagaaaatcg actatatttg gaattctttg ttaatgatag atatatttct agtattttta
    24001 tattcctata catttaatgt aaaggtaggg ttttttgata agatcagcaa aaatagatga
    24061 ttcagttgca attactaaaa ttcatttaga aactcttccc aatgatctac ttccaagact
    24121 tggttttagt tttttaaaga attattatca taatttattg tcatcatcgc aagctctagt
    24181 gatagtaaat gaggatagtg gtcagataaa ctcctttgtt atatttgcat ataacacttt
    24241 tgagttatct aaattgataa agaaaaacaa aatattttta gcatactcca ttctaaaagc
    24301 tacatttagc gattttagaa ttccgattct agttctaaat tttatttttg gaaaggaaaa
    24361 atataatata aagattgagg aaattgaaac tataccagaa ttatattcga ttgcaacagc
    24421 aagcaattat aaaagtaaag gtataggtgc tgccattaca aagttcggtt tagagatctt
    24481 gaaaaataaa acgcatagac aaggttgcat tgttaagact tcttcaaaaa gagctaaaca
    24541 attttacgaa agactcggct ttttagagat tggagagaca ataagaggta ataataaact
    24601 ctctattctt tatattccat ttgaggaaat aagatgatac caagattaaa accatttatg
    24661 ggaatcgatg atttattcgc tgcgatttct tttttcaaaa gtaagtctgt tgaaaagttt
    24721 gaatctgagt ttgcaaagaa agtaaatcag aaatatgcaa ttgcatttcc ttatggaaga
    24781 acagcattga tgattttatt ggaagctatg aatttaaaaa agaaagagat tatatgtcct
    24841 gcgtatacct gtatagtggt accgcatgcg attgtattta gtgagaacga tccagtcttt
    24901 cttgattcca atccaaatga ttttaatatg gatttaaaaa aaattgagcc attattaaat
    24961 aatgatactg gtgcaatagt ggcgacttcc atttttggtt acccaattaa cttagacgag
    25021 ttgaagcgaa ttaagaaaaa atatccaaat attaaaatta ttcaagattg tgctcatagt
    25081 tttacagcaa attatcatgg tgaattcgtt catactgtcg gcgatgctgc tatatttgct
    25141 atgaatatta gcaagcatat gaattctatc tttggaggaa tggtaacaac agatgatgct
    25201 gatcttgcaa agaaactgat tcatttaaga gattcaaaat taaaatcacc tccatttaaa
    25261 aaaaccttat ttcgattagc atatttattt gcagtttatc caacatttca tccttttctg
    25321 tatggtattg ttaattggat ggaaagaaaa aaacttctag gcaagttagt tgagaaattt
    25381 gattcaaaaa tttttatgcc tgatgattac ttagataaga tgattgatgt agaagctaga
    25441 gttggtataa gtcaactcaa aaaaattgat agaattattc aagatagagt ggatgcagta
    25501 aattattatt ttgattcttt gaaagatatt aaagaaattc aattgcctcc taaaatagaa
    25561 ggagctactt actcccattt tacgattcaa gtagaaaata gagataaatt aatttcatat
    25621 atgttagata aagcaataca attagggcag ttgattgagt attctgttcc agaattagac
    25681 tcatataaaa atcgaaatta tagagtcaga cctgaatgca atgttgctag taattttgtt
    25741 cccaaggtag tcaatttacc tatttcttca ggtatgaaag atgtgcaaaa agtcgtgtcc
    25801 gaattgagga aatactatgg tatctaaaga tagtatttct aatgtaaatt aatagatgct
    25861 atttttttaa ttgattgact atatataatg gtagtatttg tactctaaat tgatagattc
    25921 ttaggatttt tcatactttt atagaaaaaa attacactta tgtcaattta taacccaaat
    25981 attataactc tctatttaaa actattttat ctcacagcca tttattgcct aacaggttat
    26041 cttgtaattg gcaagtttct aaaaaagtcg atttttttta gtttagcttt ttttattttc
    26101 tttggtgtat gcatacaatt tttactaggt tttttattag ttgaattgaa tataaaaaca
    26161 aaatatctac cactcttact tcttccttta tttttctatc gcttttttct tttttataag
    26221 aataaaaaag ttaatctaga aagtatagaa attttaaaaa accgatctag tattcgagaa
    26281 gtcactattt tttttatttg cctcttacta tcttcctctt tttattttat tccattcatt
    26341 tccgagaaga cttctggatt ttatgcgtac ggtggcggtg accagactag ttattttggt
    26401 gtatctgaat ttgtttatga tactactctc aaagaaaatt tttatcactt atcaaagtat
    26461 tctaatcaat ataatttttt atatgaaaca aattttcctg taaagtcgct atcttattat
    26521 ttatttttga aagcaaattg ggttttagcc ccccaattta tcgcagtctc tcctatttcc
    26581 tttgctccca cttatcctga agaatcctat tctgcattta tagcaatttt acttttttcc
    26641 ttgaattcgt ttatagtatt tttactatct aaatttttaa taagagtttc taaaaaaaaa
    26701 tatctaatat tctttttttt aactatgatt gcatccggtg gacttagctt ggcttggaaa
    26761 catgctacac ccgctttgtt ctcttggata tcttccttat ctattttagt aatactttta
    26821 ttaagtaagg aatatcatat tgaatttctt aatccattta ttatagcaat tcttacactt
    26881 gtatcatttc tactttactt accttcttta gtattagttt ttattccctc tctatacttg
    26941 tttataattc ttcttcaaaa aggtaaaata gcctttgctt ttacaattca aacagggatt
    27001 atttttttat tacttggtaa tctaaatcta cagtatccag ttagattaag cttaggaaat
    27061 gcctttgaag atagaatgct tctagattat ggtttaaaat atgaacaatg tataaaagca
    27121 ttaactggta tttatgattt cgagtcttta ttgtcctttc caataaagaa tagtcgtagc
    27181 tttgaaattt tgatagtatc aatcgttttc attggttttg ttatacatat tttttgggga
    27241 aaaagcaaaa gaaatatagc tgttttacta acgatagttt tatcgttatc cttatccgtt
    27301 tattactatc taaaaaatgg tcattatcat gtaatccgct taattgaatt ttgctctatt
    27361 ttagtttttc ctcttagcgc ctttacgtta gtaaatttta tttcaaataa aagaaataca
    27421 atatttctgt taataaggaa ttattttttt atattcctaa tttctgttct tgtatttgga
    27481 gtaattaaaa atagactctt tgtttttaat ttatttaaaa accctgatta taatgcaaga
    27541 gcgtccctaa aaaattcaaa cagcctcttg ctgacaaatg atcttaagaa aatgaatacg
    27601 cattcggaga aatacttttt ctggatgcac catggtagtg ttcaatctgc aaataatcaa
    27661 attatgttta gaaatgtaaa
//
LOCUS       DAOQOS010000075        27312 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_804_length_27312_cov_6.431852, whole genome shotgun sequence.
ACCESSION   DAOQOS010000075 DAOQOS010000000
VERSION     DAOQOS010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 27312)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27312)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27312
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_804_length_27312_cov_6.431852"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            225..641
                     /locus_tag="PLF66_15945"
     CDS             225..641
                     /locus_tag="PLF66_15945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65586.1"
                     /translation="MLYTNKELETHFPLSERMKLEKMKAQNSPVYWMNEYIAASIEGA
                     DDVQSLVSYSKNVTEQLESVYADIEPFLKKTSDIRLLADYIRSLEDQVEDLYQEKEEA
                     ILSFEGVKSISDLVNLAKGMEEQLKSLYKEREENIQ"
     gene            651..1019
                     /locus_tag="PLF66_15950"
     CDS             651..1019
                     /locus_tag="PLF66_15950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015679238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="photoactive yellow protein"
                     /protein_id="HMZ65587.1"
                     /translation="MEDFAKGILYNLGYLSRDQADAANFGIIKVDDQGQILLYNRYES
                     ELAGVPVHNAEGKNFFTQVAICTNNRLFFGKFKDGVSSGHLDISFNYVFTYKMKPTNV
                     VIQLYRDQASATNWIFVKKK"
     gene            1081..1443
                     /locus_tag="PLF66_15955"
     CDS             1081..1443
                     /locus_tag="PLF66_15955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65588.1"
                     /translation="MTNLIQDNPELTEKQILQLKYNIKLKIRALGAIISINKDSDVEE
                     VNLAIRNFWFELRSEWIRHNMINNYNMVMTGTADVVSVIQSAFISGMIGELENFIPEN
                     ELEKMNEIMVKVQYGGEL"
     gene            complement(1438..2247)
                     /locus_tag="PLF66_15960"
     CDS             complement(1438..2247)
                     /locus_tag="PLF66_15960"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HMZ65589.1"
                     /translation="MIRIIPIFLTLFFSIPVLSETKWIKSLDKGISIAKEENKPILVD
                     LYTDWCLYCKDLENKIFPSKDVATELEKFVTVRINGDESQDIVSKYSVRGYPTILLLD
                     KNGNYIEKITGLPNNALLITKMQEAYIKKDIESNLLAEQKNSPDSVLSNFNLGVFYYR
                     NEKLKSSLDYFLKSYNATEKDNEEKRQEALFMLGLIQLQEKNFSESISIWNSYMEKYP
                     ENKKGSALYCRGISYYFAGKKQEAKSDLLKAKEFSTSKDMQDKIQMFLSEL"
     gene            2356..2643
                     /gene="hisE"
                     /locus_tag="PLF66_15965"
     CDS             2356..2643
                     /gene="hisE"
                     /locus_tag="PLF66_15965"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HMZ65590.1"
                     /translation="MKFLEELEELLKQRKKDLPEKSYTADLFKRGLDRILKKIGEEAG
                     EVIIAAKNTNESELIHEVADLLFHVQVLLVDKNISLSQIETELKKRHQVKE"
     gene            2651..4078
                     /locus_tag="PLF66_15970"
     CDS             2651..4078
                     /locus_tag="PLF66_15970"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HMZ65591.1"
                     /translation="MLFNSFLFLIFFLIVYLLYHNTGEKFRKPILFISSILFYGSWDI
                     IAFDSVIPRFLIHFLTVITINFFFLKWMEHSKTQESKKVPLTIAIVLNVLNIAFFKYF
                     YFLAEIIGLLIGKPNLKSVAVENISIILPIAISFYTFQIIAYLVDYYRGMISEKNSFF
                     DFAIFILFFPQQLAGPILRANEFLPQLNSKRVLTDNQITEGIGLVGFGLLKKVVLADS
                     MARILDPIFANPSAYSGKAILCALIGFFFQLWGDFSGYSDIARGCGKLLGFELPKNFT
                     GPFFSQSFREMWTRWHITLSSFLRDYIYFPLGGSKGSSFRTDFNNLVTMFIAGVWHGA
                     NWNYVIWGLVIGGSLVFERHILNRFHFWAESKTKAAVLVRFISISLFWFLIADIFRVS
                     SLKDFAIMIERTFYWMSGEKVQYEEFGYLCVFFFILHAIEYNPSLVTSRIKKPIVAIV
                     ITSILFLFVISTLSNRQVQFFYFQF"
     gene            4082..5197
                     /locus_tag="PLF66_15975"
     CDS             4082..5197
                     /locus_tag="PLF66_15975"
                     /inference="COORDINATES: protein motif:HMM:NF019243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1574 family protein"
                     /protein_id="HMZ65592.1"
                     /translation="MKLKYLPILLLLFLFAIDKIFIIPSVRKAIVQNHVNSYEVLNNL
                     LDKLYKEHIEKYKKISNLSADEVEKETIIFMGTSRSVEFANMKDSLFDKNPFVKNAAA
                     TSRIPVTSWTIRAAPFIHIYQIYNHFINTHTRPKLVVVEVSYPSFNKNNIFKKKSDIH
                     DFTFDEFLDSHGEFSNADKIEYLSSVFFVLNRSNINWKGLLSSGINTNTDVNATLDFV
                     LDAKKLMEMRENQGSWMQGTKENQEDEALVNTYQKYTDWVLQSFYQNVQIDLTSFNLL
                     KKIVREAKQKNVNLVLYRARTHSSLKKATARYAKKEEIWLQEVTKLAKENSVSFYDYE
                     TSGALECDYFVDTSHMSKSCFPEMLEKIVEGEKKKSN"
     gene            5225..6775
                     /locus_tag="PLF66_15980"
     CDS             5225..6775
                     /locus_tag="PLF66_15980"
                     /inference="COORDINATES: protein motif:HMM:NF012447.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid ammonia-lyase"
                     /protein_id="HMZ65593.1"
                     /translation="MKDIYILENKNLSLEEFSTILEKKKEIVFSGEVLSRVKKSNSFL
                     DSLLANKVLLYGINTGYGPLATTHLPLSQAKVLQKNLIYHLSTGVGETLSPIYVRASM
                     LARLICLSKGYSGVSLKSITRLKDFLNQDIIPYIPKIGTVGASGDLTPLAHMTLALIG
                     ESKVFYKGKLIDSKIVHKNLKWKPLQLGPKDGLAFVNGTSVMTGISAVNGILSKRVIQ
                     YSIILSLLYAEILQAKEEAFFSVVARLKPHPGIQAFITQWLQYSQDSKRFKKRKQFNN
                     IKETSYENELLQDPYTIRTIPQTIGAVIDVLDSHNKTIEIELNSISDNPIFVEEDEAV
                     IHAGNFQGYSISLAADNLYNALVTIAIHSERKIARITDNKLNGGLPNFLQKNQNGLHS
                     GFMGAQVTATALVAWMRSHATPASIQSIPTNANNQDIVSMGTIASIKTSECLDRIFEL
                     LAIESLVLAQAFDLKEGELEESGFSSTSKNFIKQIRENSPFLEKDRPLSNEIMDLAYR
                     LKNGLIKI"
     gene            complement(6761..8143)
                     /locus_tag="PLF66_15985"
     CDS             complement(6761..8143)
                     /locus_tag="PLF66_15985"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HMZ65594.1"
                     /translation="MNSSSMPLQDLIFERLKEKPHPLFIFKDTIIPAATIWIGVRAWV
                     NFFRENGIKSGDRILLAYPESPAFLHILYAAIWEKLTIAIIRADNLDLPHINKIKPSL
                     IISNKQIENSISPDEMGMPPKRININKKEIPSDPKIRFILQSSGSTGNPKYICLSEES
                     IEHVIQTHSKIIESSKSISLSILPWSHCFGLVLDLFLTTFYSDTIIRDSRSGRDIDSI
                     FENFEKYHVTHFSSVPLVIERILAKEKGIQLLSSLESGIIGGAPISKKLSESLSSTKL
                     RVGYGQTEASPGICLGEKGFFRENYLGSELGCDVILSDSHELLFRGKNAFLGYWKGNS
                     IEYIPNNSWIRSGDIVEKKQEGYFFRGRLDFAFKLPNGLMIQPEELEESLAANSDLSQ
                     CLLYFKDKVYIFYNSKNKTSKEDSILKNIPTILKNYVIQITQLPETNWFYSPKGGIDR
                     KKMLTSLNLY"
     gene            complement(8234..9298)
                     /locus_tag="PLF66_15990"
     CDS             complement(8234..9298)
                     /locus_tag="PLF66_15990"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HMZ65595.1"
                     /translation="MNLTIIFLMLKEKDLEIYLLFDIFVGSATFFLFNLEERKDVLFF
                     TTLNVIGLLLYFFVDIPFIEYTPRNHNDERLLTIIFAILSFSTYCFGLYTFLKINARY
                     EKVLGNQLKSSRNLNYKLQKALRENELLYHTLNHDIKNPLSGILGMQDLLNRLLIRKN
                     IHDPDLNRYLLSLFEANSRALKIVQDFSNISEIITNTDTNLTEIHLLENLQSLVDNFS
                     GVAFNKNLQFEIICKENIKIKINVNSLNRVLENLISNALKFTPRGKKITLSVTLDQYL
                     YISFIDEGIGIPERLLKRIFDLTSTKKRNGLDNEVSTGLGLYICKELMQKLNGTISVE
                     STEGKGSNFTLSLPASIICS"
     gene            9333..9497
                     /locus_tag="PLF66_15995"
     CDS             9333..9497
                     /locus_tag="PLF66_15995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65596.1"
                     /translation="MKSRTIRKFRANKKRNKYVSIHSNRFKGRGDVMINTRITARIEE
                     FVILKKIKLG"
     gene            complement(9646..11469)
                     /locus_tag="PLF66_16000"
     CDS             complement(9646..11469)
                     /locus_tag="PLF66_16000"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HMZ65597.1"
                     /translation="MEIPSYVKNKMLVDWVKETAALCKPDKIYWCDGSEEEYNKLCQD
                     LVDAGVFKKLNPEKRPNSYIAFSDPSDVARVEDRTFICSRRKDDAGPTNNWMNPDEMK
                     SIMTKLYDGCMKGRTMYVIPFSMGPLGSDIAHIGVELSDSAYVAVNMKIMTRMGKKVL
                     DILGDKPFVKALHSVGAPLAPGQKDSTWPCNKEKYITHFPEERTIWSYGSGYGGNALL
                     GKKCFALRIASTMARDEGWLAEHMLILGVENPQGVKSYVAAAFPSQCGKTNFAMLIPP
                     KEMEGWKVTTVGDDIAWIKAKDDGQLYAINPEAGLFGVAPGTNYKTNPNAMEALAKNT
                     IFTNVALTKDGDVWWEEMTDKAPDGLTDWTGKPYDPASGKPAAHPNARFTAPISQCPS
                     VDPEYENPNGVPISALIFGGRRSSLVPIVYQAFNWNFGVYSAATMGSETTAAATGAIG
                     NVRRDPFAMLPFMGYHAADYFTHWLNFGRKLPNPPRIFGVNWFRKNKDGKFAWPGYGQ
                     NMRILKWIVERSQGKISAIESPLGWVPRHEDIDWRGMNFTREQFNEVISIDREGWKGE
                     LLLHEELFTKLYDKIPKEFIWIRELLLSGLWRSPEKWNLGE"
     gene            11666..14188
                     /locus_tag="PLF66_16005"
     CDS             11666..14188
                     /locus_tag="PLF66_16005"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HMZ65598.1"
                     /translation="MLFFIDCRPSNTTKIYSLSGEWQYKTGFDIDWLSEKSDDPSWKK
                     IKLPTNLSRELNLNSYTGYITLRKEIPHELDSRIKEGTSIAINAGRVLDVSYYYFNTT
                     LFGQLGSASPYKPGAMRPFIKDIPNKNLSDTLNRITVVLYTENGKFPLQFMDSIEIGE
                     ADDIYLAYTRKEIFAFIFLSAYFVVGLYHILLASKRPKDIYNLYFGLFCLFASIYWFI
                     ANTFSRDILFQDSVVLHRKLEHVFLFALPPTLLVFLVQFFEKRYGRFALGLTALSGTF
                     IVLSLIFPLPVMRFCAMLWQISNIVVCSYSIYFLYKHIKKGNKDGLYLIFGMLVFGLS
                     IGLDMATSRNFIHLPALSNFAFLLFVMGIAAIMANRFMKVTNEVEELNADLEMKVEDR
                     TKKLSESLDQVNKLKEQQDGDYYLTSLLLQPLSGNFIKTKETEFVTESLVKQKKQFQF
                     RKKTSEIGGDICIVDEIELEGLSFTVFLNADAMGKSIQGAGGCLVLGTVFKSILERNH
                     SNADNRNMPPEHWMKEVFRELQNVFISFNGSMLISAMFGIIDNRNGTMYYINAEHPFS
                     VLYRDRKASFIDEENVKRKIGIEVKESPLRIQLFQLKEGDQLISGSDGRDDILLSVDE
                     SGVRVINENEKLFLQIVEEADGDLIEIEKVLKQKGEITDDLSLLKITYQKKESESSSS
                     LSLEDIDKNLSEAYKCYTNGQIGKALSIYQAIFRHRKDPNLVLEITNLFIEKREYSLA
                     SRFLDIYNKQNPYNSYAIYLNSFVKKKNKEYEIAAQLGERFRLREPTNVKNLVNLADI
                     YRLLNKMELTRKYLSRALKLDPNNKQALKLEESLVLDSTISV"
     gene            complement(14407..16440)
                     /locus_tag="PLF66_16010"
     CDS             complement(14407..16440)
                     /locus_tag="PLF66_16010"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HMZ65599.1"
                     /translation="MQRFNLSLKTLFLFLLILFIFATYSCNPQDKKNYPKITKGQIDV
                     SSWNIENEPLKLEGEWEFYWNELIESNYQTANPADSLQFRKESQTHRKPIHYLKVPGS
                     WAGIEYEGNTIGENGYLSYRLLLRNVKRYEELAIRIDYIASNYKLFQNGKLLANIGKV
                     GKSEFDSSFSNLPKIISLTPTESGEIEIILHVSNFFLSNGGIFYNIYLGKLNYLEKMR
                     ENQLILDFFLFGSIGIMGFYHFGLFLLRRNDKSTLYFSIFCLLSSIRPFFVGERYISS
                     LINPEFHIYLLKAEYLNVYMLAPFFLLFQVSLFKNDFSKLITRLLNTIFITFILLVIF
                     TDSNLYTSTLSYWQILVLIVFLYILFCIFRAAFNKREGALPFLSGLLCVILASVNDIL
                     HVQEIIQTGYYFPIGLLVFIISQAYILSANFSNAFKQSEISREEAEMQKKLAQKREVE
                     IKQLNKAKDEFLANLSHELKTPLTYIYMYSEILSTTNEKETVIGYAKDLLANSKKLKE
                     YLEDLILITDLETNIQLNLEKKSLQELVRIELDKLNDLAKEKQVQIKLEIPKSIDVNV
                     DKKIFGKAIYSILKNSIVFNKINGLISIGARFDEESIFLSITDTGIGIEKKYLDKIFD
                     KFFRIDSSLSYKVSGVGIGLYLAKKTIELHQGHLTVLSELNIGSTFTIQIYRF"
     gene            complement(16427..20146)
                     /locus_tag="PLF66_16015"
     CDS             complement(16427..20146)
                     /locus_tag="PLF66_16015"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HMZ65600.1"
                     /translation="MKSQSKFNLFLIYFVLFLCLGFWNFIYADDSVERPKAVKGILDL
                     RSWNFNAKDFEEGITNLDGEWEFYWEELIDPNETTTRNNRSYLNLPVQWQRIGYPEYG
                     YASFKIKVLLPEAKQSLSFAMPDTGTAYRLFINQKLISQNGKVGKSKQESEPFLQYKT
                     FEIPTERNELDILLHVSNFHYNKAGPWTTIKIGATEKINARAKRTFALDLIVFSSLII
                     MGLYHLGLYMSRRKDTSAFHFGIFCILIALRTISTNELILYEAFPFLPFTIFHRIEFI
                     SFYYGGFSFALFINSLYPGEFSKKILFFIGLFEIPASLSAIFFSMKVYSFFLIYNQYL
                     ILLVIFYTLYVLILAAFRNKVGAKLFLLGFSLFSVSLISDITRSLGLNQLPALASYGL
                     LIFVLFQATVLSRRFANTFHQSEMLSEELRKFSSNLESKIEERTLELVTLNEISRTVN
                     RSNNIQAIIDWTMNHISKNYDIDMFTLSMVEADRLSAKVLYFNLPPNSDIDELNKFVY
                     SIRIPLNDIDHVHGYVFHTKQALYTKELVESKRDFEKKLIEIFQFRSYFCLPLILKEE
                     VIGFLGLSNFGREMILSESEIESLKKIADQITGAVYNSKLLEESERVKNEIQSLNEIT
                     KNINSVSTLTDIMSFVMYYLETDLDYKDFWLILPDKNRNEFRTFSFVSNSLKKSAIDS
                     IKSWSAPINEQTGFIYETFNSQQFYFFEFNDFDTFTPTDQWILETTNWKYFLLLPLVI
                     YGEVIGILSINKQLTNESILPEEIERLINFSDQIAGAIANAQILKESEEAKKSAEVEK
                     EKADLARKEIEYINEFAKVINSERSLDNIFSFAVDNLKQKFDANIFWLQLVTRDKLRL
                     FTRCLISNELEDSTIQKYKNLNAELKPELGSMYLTYFMKKTFYLRDVTQINIDNMSEI
                     DREITEALKIKSVLQIPLLVQNEVIGILHINKIHGMQKFNKQDIKFIESFSEQLAIAV
                     NNSFLYEEVESKREEAEIEKKIAVMAQLEAEKERDKSERLLLNILPRQVAKELKEKGF
                     SAPVYFESATVLFTDFKGFTQIAETMSPVDLVKELDSCFGQFDKVTERYGLEKLKTIG
                     DSYMCAGGIPKANKTHAIDAILAALEIQNFMNQMKEIKEIIGAPYWELRLGIHTGPLI
                     AGVIGEKKFAYDVWGDAVNTASRMESSGTPGKINISQSTYELIKDFFSCELRGKIQAK
                     NKGEINMYFVNGIKEDLSTEGSIPNSKFWNLYAKI"
     gene            complement(20143..22116)
                     /locus_tag="PLF66_16020"
     CDS             complement(20143..22116)
                     /locus_tag="PLF66_16020"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HMZ65601.1"
                     /translation="MFSIKSINGFLIVFAFLFSISCSNENKEILRMENGVLDLQSHNF
                     NQNKSLEISGDFEFYWNEFIHQKDISHHKLNSISASHRWNDSLSSNNKIGSNGYATYR
                     LTILNNKFNDLAINTNGIHSSYAIYINGELVDQSGRVGKSKEESDFKIKPVLLKIPSG
                     EKVEILFHVSNFFHLDGGLKNPFFIGKTKTLKEEKLNRILLDFFLFGALFIMSILHFI
                     IYLLRRSDKSAIYFAFLCLFFSVRPFFRGERILAAVFPYIDENILHKLDYILLFTAFF
                     YILSFLKELFPNEFKTKAFNVYSYILLVFNLIFLLTPIQFSYSTLSAFYILTITALLH
                     GLVSISKAIIYKREGAVSFCVGILALSIFSINDILYTKGILNTGHFIYFGVLVFIFSQ
                     AYLLSSKFSKAFKQSEISRAIAIEQRNLAEKRRIEIELLTNTKDQFLSNLSEELKKPL
                     ENIYSDSEILITQTDQINPTKLGKSIFTNAKILNRYVEDLILTTDLESNIKLNLEYCS
                     VKEILDAEIKNLIELAEPKKIKIENHVSYALKINADRKLLEKLFYEILKNSIQYNTDF
                     GSVYIKEEIRADYIIISIQDTGIGISQDHQKKIFDKFFRINSFISYDVIGAGLGLYIA
                     NKIASLHNGKLEVSSILEEGALFKAYFPRGNNK"
     gene            complement(22122..22487)
                     /locus_tag="PLF66_16025"
     CDS             complement(22122..22487)
                     /locus_tag="PLF66_16025"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HMZ65602.1"
                     /translation="MKKILIVEDQAPMAKILADIFKKENYDVITANNGQIGIDKAKEI
                     KPDLIITDIMMPVKTGLDLLKELRDLPEFAATPIFVITAKGGTTDSKTAADAGASSFI
                     TKPFSPGQIVLEIKKFLGD"
     gene            complement(22484..24145)
                     /locus_tag="PLF66_16030"
     CDS             complement(22484..24145)
                     /locus_tag="PLF66_16030"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HMZ65603.1"
                     /translation="MKLTLLLETFTHIVPLPFGIYFSVVSGSGSYLAVEEYKYVATAG
                     IVNAILMVILGTLWRFISLKKIFSQILEIYKSENVPKEKQVSLKLAILRYPFREGKMI
                     VIRWMTGVIGSHLVAILILNDIRPALHLTAPFLFLMVSPISYIAYFFITENELKKVFQ
                     LRKIKRIQIQKEHIFQFNSFQRILFTLIAIITMPIIVLGYMLYGSISQLIRLENITLH
                     IITMSVLFPIPVLVTGYVVAKTFRNGISSVNTHLDQLGKGNFSIVSMPTSSDDFGLQA
                     IHLNNIIQKLNSLYKETIELNESLESKVIDRTSELQKALKEMETLAESRKRLSVIGEM
                     ASGIVHDIKNPMTTIKAYADLTMSVHIDPSKKSEYLKLILREIDRLSDMTYEILDFSK
                     GNFRLNLYPHSLCEFLRELYDFLIIDFQYAGINLSIECNSYERILFDKERIRRVIINI
                     ANNAREAMHDSKKEYFLKIKSENLNTGIHLHLEDNGPGLPNSIEEKIFEVFATEGKAK
                     GNGLGLYMSKKIIEEHNGTISYKTSKNLGTTFTIFLPKRNEDSKI"
     gene            24340..26862
                     /locus_tag="PLF66_16035"
     CDS             24340..26862
                     /locus_tag="PLF66_16035"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HMZ65604.1"
                     /translation="MKLFHSILYLLIVLIFSNCKQSLSSISDIPIEWEYADSEQYEPL
                     SFLGDKEFKAIDANKVHLLSQFVPNEKGYIWLRGFFNYNSSNEPISILMEKIVWAEQV
                     YLNGHYLGGTIQREKEQWNYWNLYRNYPIAKDSLIDGKNEILLRVYVESEGSVPDRIM
                     VGPRVAIDELAFVKMFYESYLNGIVTVLFIVIALYHSLIYWKRKQDVENLYYAIFCFT
                     YSLYGLNFVGWILGLFLKVNYFYFQKFIFLSMYISAFALYRFIAIFLKRKDRKWFNYL
                     YMILIALPILLIIASPNYSFMYKTRGYIMFFVIPFLLYILLVPIYNYIKYKNAEAKTM
                     LASLVIIFSVALHDILNVIFKWNSFFWLGPGIPLFMGSIMFLLGNKFVDVYNQTDELN
                     ETLEQKVVERTKEVMEKMDVIKALNIQQDGDYYLTSLIERPLGTNYNKSKNVKTDFYV
                     EQKKRFQFKNRNSELGGDICISGNLRFGDGKDRHVFFFNGDAMGKSMQGAGGAIVAGT
                     VINNILARSARNNKIQKITPAEWVTETYLELDSVFKTFDGSMLMSCSCGLVNERTGQM
                     WYFNAEHPWAVIYRDGKAEFIESGLNLRKLGTIIDGNVFNIQEYQLYSGDILFVGSDG
                     RDDLDISHNAEREINEDETRFLRAVEKSGGNLTKLVEAIHSIGKLTDDLSLIRIEFLA
                     KQDSDTVVKNLFEEGKSLIEAGNVSEGLAKLLSHLELKPDDLANLELIANTYYHIKDY
                     QEASFYMEKILEMTPTDTDTIFNLSLCHKHMKDIEVSTQYAEQLFQLNPKKISNVINL
                     ADNYRILGNLEKSRETLNLAIDSISYFENAIKLDRLLKAKGY"
     gene            26929..>27312
                     /locus_tag="PLF66_16040"
     CDS             26929..>27312
                     /locus_tag="PLF66_16040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65605.1"
                     /translation="MNRCNNHLPFQYILDRCQGLILISCLLITTNLFSAEMLAVAMAK
                     VGNAVYERNNKQAQITKGMIFETKDKVITTNGIVDIQLGQSSIIRVGKNSVVLISKLI
                     EENGSEKTELNLNKGIIFSKISKKLD"
BASE COUNT         9092 a         4148 c         4540 g         9532 t
ORIGIN      
        1 aggtgaaacg atctctccta actggaccct cgaatttttt ttaattaagg aaaattagaa
       61 tactgtgaat ttagatcatt ttaaaaaaat tgaaaggctt acattcacag tataattact
      121 acaaagctga gttttttagg aaattaacgc ggtatatttg gtattcagtg aatttcaaat
      181 aaataatact tgaaaaaaaa acagatggtg ttatatacga cggcatgttg tatacgaata
      241 aagaacttga aacgcatttt cctctttcag agagaatgaa actagaaaaa atgaaagcac
      301 agaactctcc ggtttattgg atgaatgaat atattgctgc aagtatcgaa ggcgcagatg
      361 atgttcagag tttagtaagc tattccaaaa atgttacgga gcaattagaa tctgtttacg
      421 ctgatattga gcctttcttg aaaaagacaa gcgatatcag attattagca gattacattc
      481 gctctcttga agaccaggtc gaagatttat accaagaaaa ggaagaagcg attctttctt
      541 ttgaaggagt caaatcgata tctgacttag tgaacttagc gaaaggaatg gaagagcagt
      601 tgaagtcttt atacaaagaa agagaagaaa atatacaata ggagtttaga atggaagatt
      661 ttgcaaaagg gattttatat aatctaggat atttatctag ggaccaagca gatgctgcta
      721 attttggtat tatcaaagtg gatgatcaag gacagattct attatacaac cgttatgagt
      781 cagagctagc tggtgtgcca gttcacaatg cagaaggtaa aaacttcttc acacaagtag
      841 cgatttgcac aaacaatcga ctcttcttcg gtaagtttaa agatggagta tccagtggac
      901 atttagatat ttcctttaac tatgttttta catataaaat gaaaccaacg aatgttgtaa
      961 ttcaattata tagagaccaa gcttctgcta ctaattggat ttttgttaag aagaaataaa
     1021 ttgagagaag aaattaaaaa actaaaatcc ctgtatgaca aatataggga gccatcgatc
     1081 ttgactaatt taattcagga taatcctgaa ctcacagaaa aacaaattct tcagttgaag
     1141 tataatatca aattaaaaat tcgtgcgtta ggtgcgatta tttcaattaa taaagattct
     1201 gatgtcgaag aagtaaattt agcaattcga aatttttggt ttgagcttag aagcgaatgg
     1261 attcgccaca atatgatcaa taattataat atggttatga ctggaaccgc tgatgttgtt
     1321 agtgtgattc aatccgcatt tatttctgga atgattggag aattagaaaa tttcatccca
     1381 gaaaatgaat tggagaaaat gaacgagatt atggtgaagg ttcagtatgg tggagaatta
     1441 taattcggac aaaaacatct gaattttatc ctgcatatcc ttgctcgttg aaaactcttt
     1501 cgcttttagt aaatcactct tagcttcttg tttttttcct gcgaaatagt aagaaattcc
     1561 cctgcaatac agtgctgaac cttttttgtt ttcaggatat ttttccatat aactattcca
     1621 aatcgaaatg gattcagaaa aattcttttc ttgtagttga atgagtccaa gcataaatag
     1681 agcctcctgt cttttttctt cattgtcttt ttcggttgca ttgtaagatt ttagaaaata
     1741 gtcaagagat gattttaact tttcgtttcg atagtaaaat acacctagat taaaattaga
     1801 caaaacacta tctggtgaat ttttttgctc tgcaagtaaa tttgactcaa tatctttctt
     1861 aatataagct tcttgcattt tagtgattaa taatgcgtta ttgggaagac ctgtgatttt
     1921 ctctatatag tttccatttt tatctaaaag caatatagtt ggatagccac gaacggaata
     1981 ttttgatact atatcttgtg actcatctcc attgatccta actgttacga atttttctag
     2041 ttctgtcgct acatctttac taggaaaaat tttattctct aagtctttac aatataaaca
     2101 ccagtcagtg tataaatcca ctaggatagg cttattttct tccttagcga tagaaattcc
     2161 tttgtctaga gatttgatcc attttgtttc agagaggaca gggatagaaa aaaatagagt
     2221 gagaaaaata gggataattc gaatcatggt gatacctctt ttgctagtat aagaattgga
     2281 tttgaaaaaa taaacttaaa aatgtgcgaa taggtattaa aaaataaagg gaaaaaggga
     2341 ataaaatctg aacttatgaa atttttagaa gaattggaag aactactgaa acaaaggaaa
     2401 aaggatttac ctgaaaagtc atatacggct gatcttttta aaagaggatt agatcgtatt
     2461 ttaaagaaaa tcggagagga agcgggtgag gtgatcattg ctgctaaaaa tacaaatgag
     2521 tcagaattaa ttcatgaagt tgccgatttg cttttccatg tgcaggtgtt actagtagat
     2581 aaaaatattt ccttatcaca gattgagacc gagctgaaaa aaagacatca agttaaagag
     2641 tgataattaa atgcttttta attctttttt atttttaata ttttttctaa tcgtatatct
     2701 tctctatcac aatacgggtg agaaatttag aaagcctatc ttatttataa gttcgattct
     2761 cttttatggg agttgggaca ttattgcttt tgattcagta attccccgtt tcttaattca
     2821 ttttttaact gttatcacca ttaacttttt ctttttaaag tggatggaac attctaaaac
     2881 acaagaatcg aaaaaagtcc ctcttacgat tgcgattgtt ttgaatgttt tgaatatcgc
     2941 attttttaaa tatttttact ttctcgcgga gatcattggt ttactaatcg ggaaaccaaa
     3001 tttaaaatct gtcgcggtgg agaatattag tattattcta ccgattgcaa ttagttttta
     3061 tacatttcaa atcattgctt atctagtgga ttattataga ggcatgatta gcgagaagaa
     3121 ttcttttttt gattttgcaa tttttatttt atttttcccg caacagttag ctggaccaat
     3181 tcttagggct aatgagtttt tgccgcaact aaattctaaa cgagttttaa cagacaatca
     3241 aatcactgag gggatcggtt tagttggttt tggtcttttg aaaaaagttg ttttagcaga
     3301 ttctatggct agaatcttag atcccatttt tgcaaaccca tctgcttatt caggaaaggc
     3361 gattttatgt gctttgattg gatttttctt tcagctttgg ggggattttt ctggttatag
     3421 tgatatcgca agaggatgtg gcaaattatt aggatttgaa ttaccaaaaa attttactgg
     3481 accattcttt tctcaaagtt ttagagaaat gtggactcga tggcatataa cgttatctag
     3541 ttttcttaga gattatattt attttccact tgggggaagt aagggctcta gctttagaac
     3601 agattttaat aacttagtta ctatgtttat tgcaggagta tggcatgggg ctaattggaa
     3661 ttatgtgatt tggggtttgg taattggagg atctcttgtt tttgaaagac atatattaaa
     3721 tcgttttcat ttttgggcag aatccaaaac aaaagctgca gtcttggttc gttttatctc
     3781 aatctctctc ttttggtttt taattgctga tatttttaga gtatcatccc taaaagattt
     3841 tgcgattatg atcgagagaa cattttactg gatgagtgga gaaaaagttc agtatgaaga
     3901 gtttggctat ctttgtgtgt tcttttttat cttacatgca attgaatata atccttcttt
     3961 agttacgagt cgaattaaaa aaccgatagt tgctatagta attacttcaa ttttattttt
     4021 attcgttatc tctacattat caaatagaca ggttcaattt ttttacttcc aattttaatc
     4081 tatgaaactt aaatatttac ccatattatt acttttattc ctttttgcta tcgacaagat
     4141 ttttattatc ccttctgtaa gaaaagcgat agtgcaaaat catgtaaact cttatgaagt
     4201 tttaaataac ctgctagata agctttataa ggaacatata gaaaaatata aaaaaatctc
     4261 aaacttatct gctgacgaag tagaaaaaga gacaattatt tttatgggaa cctcaagaag
     4321 tgtagaattt gccaatatga aagattctct ttttgataaa aatccatttg taaaaaacgc
     4381 agcggcaact tctcgtattc cggtaacatc ttggacgatt cgtgcggctc cttttatcca
     4441 tatataccaa atttacaatc attttattaa tacacatact cgacctaagc ttgttgtcgt
     4501 cgaagtgagt tatccatctt ttaataaaaa taatattttt aaaaagaaaa gtgatattca
     4561 tgactttaca ttcgatgagt ttttagattc acatggagag ttttcaaatg ctgataaaat
     4621 tgaatattta tcttctgtat tttttgtctt gaatcgttct aatattaact ggaaaggttt
     4681 gttatcgtct ggtatcaata cgaatacaga tgtaaatgct acattagatt ttgttctaga
     4741 tgcaaagaaa ctcatggaga tgagagaaaa tcaaggtagc tggatgcaag gaacaaagga
     4801 gaatcaagaa gatgaagctc tagtgaatac ctatcaaaaa tacacagatt gggtattaca
     4861 atccttttat caaaatgttc aaattgattt aacttctttt aatttactca agaaaatagt
     4921 ccgagaagca aaacagaaaa atgtaaatct agttttgtat cgcgcgagga ctcattcctc
     4981 cttaaagaag gcaactgctc gatatgccaa gaaagaagaa atttggttgc aagaagtgac
     5041 taaattagca aaggagaatt ctgtttcttt ctatgattat gaaacgtcag gtgctctaga
     5101 atgtgattac ttcgtagata caagccatat gtcaaagtct tgctttcccg aaatgctgga
     5161 aaagattgtt gaaggtgaaa aaaagaaatc aaactaatct ttcgcttttc tagtttaaaa
     5221 caatatgaaa gacatttata tcctagaaaa taaaaatcta tctctagaag aattttctac
     5281 tattttagaa aagaagaaag agatagtttt ttctggagaa gttttatcac gggtaaagaa
     5341 atctaattca tttttagatt ctcttttagc gaataaagtt ttactctacg ggattaatac
     5401 tggatacgga ccgttagcaa ctacacatct accgttgtct caagccaaag ttttacagaa
     5461 gaatttaatt tatcatctat cgactggtgt aggtgaaact ctttctccaa tttatgtgag
     5521 agcgtctatg cttgctagac tgatttgtct ctcaaaagga tattcgggag ttagtttaaa
     5581 atcaattact cgtctaaaag attttttaaa ccaagatatc attccgtata ttccaaagat
     5641 agggacagtg ggagcaagtg gggatttaac tcctcttgct catatgactc ttgcgttgat
     5701 cggagaatca aaggtttttt ataaaggcaa attaattgat tcaaagatag ttcataaaaa
     5761 tctgaaatgg aagccattgc aattaggtcc aaaggatggt cttgcctttg taaatgggac
     5821 ttcagttatg acaggaattt ctgctgtgaa tggaattctt tcaaaaagag ttatccagta
     5881 ttctatcata ctttctttgt tgtatgcaga gatcttgcag gcaaaagaag aagctttttt
     5941 ttctgtagta gccagattaa aacctcaccc tggcatccaa gcatttatta ctcaatggtt
     6001 acaatactct caggattcta aaagattcaa aaaaagaaaa cagttcaata atataaaaga
     6061 aacttcttat gaaaatgaat tattacagga tccttataca atcagaacga ttccacaaac
     6121 aataggcgct gttattgatg tcttagattc tcataataaa actattgaaa ttgaattaaa
     6181 ttcaatttca gataacccaa tttttgttga agaagatgaa gcggttatcc atgcaggaaa
     6241 ttttcaaggt tattcaattt ccctagctgc ggataatcta tataatgcgc tcgttacgat
     6301 tgcaattcat tccgaaagaa aaattgcccg tataacagat aacaaactca atggtggact
     6361 tcctaatttt ttacaaaaaa accaaaatgg attacatagc ggttttatgg gtgcacaagt
     6421 tactgctaca gctcttgtgg cttggatgag aagtcatgca acacccgctt cgatccaatc
     6481 cattccaacc aacgcaaata accaagatat agtcagcatg ggaaccattg cttctattaa
     6541 aacatctgaa tgtttagata gaatctttga actacttgca atagaatcat tggttcttgc
     6601 acaggctttt gatctgaaag agggagaatt agaagaaagt ggatttagtt ctacttccaa
     6661 aaattttata aaacaaattc gtgaaaattc tccctttcta gaaaaagata ggcccctctc
     6721 caatgaaatt atggatcttg catatcgttt aaaaaatgga ctaataaaga tttaaactag
     6781 ttaacatttt ttttctatca attccacctt taggtgaata aaaccaattc gtttctggaa
     6841 gttgtgtgat ttgaattaca taatttttta gaatggtagg aatgtttttt agtatagaat
     6901 cttctttaga tgttttgttt ttgctattat aaaaaatata aactttatct ttaaaatata
     6961 gcaggcattg cgacaaatct gaatttgccg ctaaggattc ttctagttct tctggttgga
     7021 tcataagtcc gtttggaagt ttaaatgcaa aatctagtct tcctctaaaa aaatagcctt
     7081 cctgtttctt ttctactatg tcaccagatc ttatccaaga attatttgga atatattcta
     7141 tcgagttccc tttccagtat ccaagaaaag catttttccc tctaaacaat aattcgtggc
     7201 tatcagaaag aatcacatcg catcctaact cacttcctaa ataattttct cgaaaaaatc
     7261 ctttttctcc caaacaaatt ccgggtgacg cttctgtttg tccataaccg actcgtaact
     7321 tggttgagct gagtgattca gataattttt ttgaaatagg tgcgccaccg atgataccag
     7381 attcgaggga agataataat tgtattcctt tttcttttgc taatatcctt tctattacta
     7441 aagggacgga tgaaaaatga gtcacatggt atttttcaaa attctcaaaa attgaatcaa
     7501 tatctcttcc actccttgaa tctcgaatga ttgtatcaga atagaacgtt gttagaaaga
     7561 gatctaatac aagaccaaag caatgactcc aaggaaggat agacaaagaa atacttttac
     7621 tagactcaat tatttttgaa tgagtctgaa ttacatgttc tatgctttcc tcgcttagac
     7681 atatgtattt aggatttccg gtagacccag aagattgaag gataaaacga atctttgggt
     7741 ccgatggtat ctctttctta tttatattta ttcttttagg aggcataccc atttcatcgg
     7801 gcgagatgga attttctatt tgtttatttg atataattag actaggtttg attttattta
     7861 tatgcggtaa atctaaatta tccgctctaa taattgcaat agttaatttc tcccagatcg
     7921 ctgcgtatag aatatgcaag aaagcagggc tttcgggata ggcgagtaga attctatcac
     7981 cgctttttat tccattttct ctaaagaaat ttacccaagc tctcacacca atccaaatgg
     8041 ttgcagcagg tataattgta tctttaaaga taaacaatgg atgaggtttc tcttttaatc
     8101 gttcaaaaat taaatcttgt aaaggcatac tactcgaatt caatattgga tttaattaaa
     8161 ttagaaaatt tagtaaatac atttctttca atgagttaaa aattttagtc ctacaatctt
     8221 tttaacaaaa tatctaggaa caaataatac tagctggcag gctgagcgtg aaattagatc
     8281 ctttgccttc tgtgctttct acactaatcg ttccatttaa tttttgcatg agttctttac
     8341 atatataaag tcctaatcca gtcgaaactt cattgtctaa tccatttctt ttttttgtgc
     8401 tggttaaatc aaatatacgt tttaaaagtc tttcgggtat accgattccc tcatcaataa
     8461 atgaaatgta taaatactga tctaacgtca cagaaagtgt aatttttttt cctcttggtg
     8521 tgaatttcaa tgcattgcta attagattct ctaaaactcg attgagtgaa ttgacattta
     8581 ttttgatttt gatattttct ttgcaaatga tttcgaattg taaattttta ttaaatgcta
     8641 caccagaaaa attatctaca aggctttgta aattttctag tagatgaatt tcggtcaaat
     8701 ttgtatctgt atttgtaata atttctgaaa tatttgaaaa atcttgcaca atttttaagg
     8761 ctctgctatt tgcttcaaac agagaaagca aataacgatt caaatcagga tcatgaatat
     8821 tttttcgaat taagagtcga tttaataaat cttgcatacc tagaatgcca gagagtgggt
     8881 tttttatatc atgattcaat gtatgataaa gcagttcgtt ttcacgaagg gctttttgta
     8941 atttataatt taagtttctg cttgatttta gttggttgcc taggactttt tcgtatcgtg
     9001 cattaatttt taaaaaggta tataaaccaa agcaatatgt gctaaaggag agtattgcaa
     9061 aaataattgt aagtaatctt tcgtcattgt gatttcttgg ggtatactca ataaaaggaa
     9121 tatcaacaaa aaaatacagt aacaaaccga tcacatttaa agttgtaaaa aataaaacat
     9181 ccttcctctc ttccaaatta aaaagaaaga acgttgctga tcctacaaat atatcaaaca
     9241 atagatagat ttctaagtct ttttctttta acattaaaaa gataatagtt aaattcatag
     9301 aaagagctag aattcttttg gcaatttgaa aattgaaaag ccgaacgatt agaaagttta
     9361 gagcaaataa aaaaaggaat aaatacgtta gtatccattc gaatcgattt aaaggaagag
     9421 gtgatgtgat gataaataca agaatcaccg ctaggattga agaatttgta atattaaaga
     9481 aaattaagtt aggctgatgg gcattgggga cttttttgat cccaaagaat actatatttt
     9541 taagaaaatt ttttattttt tttaagatca tgaaaattgt atttcatgtg agaaataaaa
     9601 aagggattgt gcaagtaaaa cacaatcccc ttttaaaatt agaatttatt cgcctaaatt
     9661 ccatttttct ggagaacgcc agagtcctga tagaagtagt tctcgaatcc agataaattc
     9721 ttttggaatt ttatcataga gtttagtgaa aagttcttca tggagtaaaa gttctccttt
     9781 ccaaccttct cggtcaattg agatcacttc gttgaattgt tctcttgtaa aattcattcc
     9841 tctccaatcg atgtcttcat gtcttggaac ccagccaagt ggagattcga tagcagaaat
     9901 tttaccttga gaacgttcta cgatccattt taggatacgc atattttgtc cgtatccagg
     9961 ccatgcaaat tttccgtctt tgttttttct gaaccagttt acaccaaaaa ttctaggtgg
    10021 gtttggaagt ttacgtccaa agtttaacca atgtgtaaaa tagtctgcag catggtagcc
    10081 catgaatgga agcatagcga aaggatcacg tctaacgtta ccaattgctc ctgttgcagc
    10141 agcagttgtc tcagaaccca tagtcgcagc agagtaaaca ccgaagttcc agttgaatgc
    10201 ttgataaacg ataggaacta agctacttct tctaccaccg aaaataagtg cacttattgg
    10261 aacaccattt ggattttcat attcagggtc aacagaagga cattgactaa ttggagctgt
    10321 gaatcttgcg tttggatgag cagcaggttt tccacttgca ggatcatacg gttttcctgt
    10381 ccaatcggtt aatccatctg gagctttgtc agtcatttct tcccaccaaa cgtcaccgtc
    10441 tttagtgaga gcaacgttag tgaaaatagt atttttagca agagcttcca ttgcgtttgg
    10501 atttgtttta taatttgttc ctggagcaac accaaaaagt cctgcttctg gattgattgc
    10561 ataaagctgt ccatcatctt tagctttgat ccatgcaatg tcatccccaa cggttgttac
    10621 tttccaacct tccatttctt ttggaggaat gagcatcgca aagtttgttt taccacattg
    10681 gctagggaaa gcggcagcaa cataggattt tactccttgt ggattttcaa cacctaagat
    10741 cagcatatgt tctgcaagcc aaccttcatc tctcgccatt gtagatgcga tacgtaaagc
    10801 aaagcatttt ttgccgagta gtgcatttcc accataacca gaaccataag accaaatagt
    10861 tctttcttct gggaaatgag tgatgtattt ttctttgtta caaggccatg tagaatcttt
    10921 ttgtcctgga gcaagtggag ctcctacgga gtgaagtgct tttacgaatg gtttatcgcc
    10981 aagaatatct aaaacttttt tacccatacg agtcatgatc ttcatgttta cagcgacgta
    11041 agcagaatca gataattcta caccgatatg cgcgatatct gatccaagtg gtcccatgct
    11101 aaatggaatc acatacattg tgcgaccttt catacatcca tcatataatt tagtcatgat
    11161 agacttcatt tcatcaggat tcatccagtt atttgtaggt cctgcatcat cttttcttct
    11221 agaacaaata aaagttctgt cttctacacg cgcaacgtca gaaggatctg agaacgctat
    11281 ataagagttt ggtctttttt caggatttag ctttttaaat actccagcat caacgaggtc
    11341 ttggcaaagt ttgttgtatt cttcttccga tccgtcacac cagtatattt tgtctggttt
    11401 acagagggca gcagtttctt ttacccaatc gaccaacatt ttatttttta cataagatgg
    11461 tatttccatg tataattctc ctcatgctta catctaagta ctatttgatt attctatatc
    11521 ttttttcaaa gaactttttt ctcaatttaa ggtcacaaaa atactgagaa ttatcaggat
    11581 tttaaaattc ctctcttttt attcttgctg atttagaaaa attctatata aatgccgaat
    11641 gaagatttta acctgtatca tttttttgtt attttttatt gattgccgcc catcaaacac
    11701 tacaaagatc tactcattga gcggggaatg gcagtataaa actggatttg atatagattg
    11761 gctaagtgaa aaatcagatg accctagctg gaagaaaatt aaacttccaa caaacctatc
    11821 tagagaatta aatttaaatt cttatacggg atatattact ctcagaaagg aaattccaca
    11881 tgagttagat tctcgaatca aagagggaac atcgatcgca atcaatgcgg gaagagtgtt
    11941 agatgtttcc tattattatt ttaatacgac actttttggt caacttggat ctgcaagtcc
    12001 atataaaccc ggagcaatgc gcccttttat aaaagatata ccaaataaga atttatcgga
    12061 tacactgaat cgaattacag ttgttttata tactgaaaat ggaaaattcc ctctgcaatt
    12121 tatggattca atcgaaattg gagaagcaga tgatatctat ttagcatata ctagaaaaga
    12181 gatatttgct tttatatttt tatcagctta ttttgtagtt ggtttatatc atatccttct
    12241 tgcgagtaaa agaccaaaag atatttataa tttatatttt ggtctttttt gtctttttgc
    12301 ttccatctac tggtttattg caaatacatt ttcgagagat attctatttc aggattcagt
    12361 agttcttcat agaaaattag aacatgtatt tttatttgct cttcctccta cacttttagt
    12421 tttcctagtt caattttttg aaaagagata tggaagattt gcattaggtc taacagcgtt
    12481 aagcggaacg tttatagttc tatctctcat ttttcctttg cctgtgatgc gtttttgcgc
    12541 gatgctatgg cagatatcta atatcgttgt atgttcttat tctatttatt ttctctataa
    12601 acatattaaa aaaggaaata aagatggtct ttatttgatt tttggtatgt tagtctttgg
    12661 tctttcgatc ggactagata tggcaacttc tagaaatttt attcatttac cagctctttc
    12721 caattttgcc ttcttattat ttgtaatggg aattgctgct atcatggcga atcgttttat
    12781 gaaagtaaca aatgaagtag aagaattgaa tgctgattta gaaatgaagg tagaagatag
    12841 aactaaaaaa ctctctgaaa gtttggatca agtgaataaa ttaaaagagc agcaagatgg
    12901 tgattattac ctaacgtctc ttttattaca gccattgtca ggaaatttta ttaagactaa
    12961 agaaactgaa tttgttacag aatccctagt aaaacaaaaa aaacaattcc agtttagaaa
    13021 aaagaccagt gaaattggtg gagacatttg tattgtagat gaaattgagc tggaaggact
    13081 gagttttaca gtttttttaa atgcagatgc aatgggaaag tcgattcaag gagctggggg
    13141 ctgtcttgtt ttaggaactg tgtttaagtc aatattggaa agaaatcata gtaatgcgga
    13201 taatcgaaat atgccaccag agcattggat gaaagaagta tttagagagt tgcaaaatgt
    13261 ttttatatca tttaatggat ccatgcttat ttctgcaatg tttggaatta tcgataatcg
    13321 aaatggaact atgtattata ttaatgcaga gcatcctttt tctgttttat atcgggatcg
    13381 aaaggcatct tttatagatg aagaaaatgt taaacgcaaa ataggtattg aagtaaaaga
    13441 aagtccgctc agaatacaac ttttccaatt aaaggaggga gaccaattga tctctgggtc
    13501 tgatggtaga gatgatattt tgctttctgt ggatgagtca ggtgtaaggg tgattaatga
    13561 aaatgaaaaa ttatttttac aaattgtaga agaagctgat ggagatttaa tagaaattga
    13621 aaaagtttta aaacaaaaag gagagattac agacgatctt tccttactta aaattacata
    13681 tcagaaaaaa gaaagtgaaa gctcgagttc tttaagttta gaagatattg ataagaatct
    13741 ttctgaagca tataagtgtt atacgaatgg gcaaattgga aaagcattat ctatttacca
    13801 ggcaatcttt cgtcatagaa aagatccaaa tcttgtatta gagataacta atctttttat
    13861 tgagaagaga gaatactcac tcgcaagtcg atttttagat atttacaata aacagaatcc
    13921 ttataactca tatgctattt atttgaattc ttttgtaaaa aagaaaaata aagaatacga
    13981 aattgccgct cagttaggag aaagatttag actcagagag ccaacgaacg ttaaaaactt
    14041 agtaaatcta gctgatatct atcgcctttt aaataaaatg gaattaactc gaaagtattt
    14101 atctcgtgct ttaaagttag atcccaacaa taagcaagct ctaaaattag aggaaagtct
    14161 tgtattggat tctactattt ctgtttgata tgaaatattt ttaaactaca ttagagaaaa
    14221 aatataaatt gataaatacg ataagaatta ttaattttta acggatcaat aaatatacta
    14281 aatgggtgaa gtaattccca aaaaagatga tatttgattg gtatttgcta aaaggcagat
    14341 tgagcgttag tagatttata actaactttt taaagaaaag aaatcatatt aaatcgaaaa
    14401 ataatttcag aatctatata tctgtattgt gaaggtagat ccaatattta attctgataa
    14461 aacagttaaa tgaccttggt gtaattctat cgtttttttg gctaagtata aaccaattcc
    14521 aacaccactt actttgtaac taagagaact atcgattcta aaaaacttat caaatatttt
    14581 atctaagtat ttcttttcaa ttcctatgcc agtatcagtt atggataaaa aaatactttc
    14641 ttcatcaaat cttgctccga tagagattaa accgtttatc ttattaaata caattgagtt
    14701 ttttaaaatt gaataaattg cctttccgaa tatcttttta tcaacattca catcaataga
    14761 tttaggaatt tctaatttga tttgaacttg tttttcctta gccaaatcat ttaatttatc
    14821 aagctctatt cttactagct cctgaagaga ttttttctct aaattaagtt ggatatttgt
    14881 ctctaaatct gttattaaaa ttaggtcttc tagatactcc tttagctttt tactgtttgc
    14941 taagagatct tttgcatatc caataacggt ttccttttca tttgttgtgc ttaaaatttc
    15001 ggaatacata tagatgtaag tgagtggagt ttttaactca tgagataagt tggcaagaaa
    15061 ttcatctttg gctttgttta attgttttat ttctacttct cttttctgag ctagtttttt
    15121 ttgcatttcc gcctcttctc tagatatctc gctttgttta aatgcgtttg agaaatttgc
    15181 tgataaaata taagcttgtg aaatgataaa tactaaaaga ccgattggaa aataatatcc
    15241 tgtctgaatt atttcttgca cgtgcagaat gtcattaaca gaagctagga taacacaaag
    15301 tagccccgat aaaaatggca atgctccctc tcttttatta aaagctgctc taaaaataca
    15361 aaagagaata tacagaaata caattagaac aaggatttgc caataagata gagtgcttgt
    15421 atatagatta gaatctgtaa aaattaccaa tagaataaac gttatgaata ttgtatttag
    15481 tagtcttgta attagtttcg aaaaatcatt tttaaataaa gatacttgaa agagtagaaa
    15541 gaaaggggca agcatatata catttaaata ttcagccttt aagagataaa tatgaaattc
    15601 tggatttatt aaactagata tatatctttc gccaacgaag aaagggcgaa tggacgatag
    15661 tagacagaag attgaaaaat agagagtcga cttatcattt cttcttaaca agaataaacc
    15721 aaaatggtag aaacccataa tcccaatcga tccaaatagg aaaaaatcta gaattaattg
    15781 attctctctc attttttcca aatagtttag ttttcctaga tatatgttat aaaaaattcc
    15841 accattgcta agaaagaaat ttgatacatg taatataatt tcaatctcac ccgattcagt
    15901 tggagtaaga gaaatgattt taggtaaatt gctaaaacta gaatcgaatt ctgattttcc
    15961 aaccttccca atatttgcga gtaatttccc attctgaaaa agtttataat tggaagcgat
    16021 ataatcaatt cgaatcgcaa gttcttcata tcttttgaca tttcgcaata agagtctata
    16081 gctcaaatat ccattctcgc cgatggtatt tccctcatat tcgattcctg cccatgaccc
    16141 tggaacttta agatagtgaa ttggtttgcg gtgagtttga gattcttttc gaaattggag
    16201 agaatctgcg ggattagcag tttgataatt agattcaatc aattcattcc aataaaattc
    16261 ccattcccct tctaatttta aaggctcatt ttcaatattc caggagctaa catcgatttg
    16321 accctttgta atctttggat aattcttttt atcttgtgga ttacaactgt atgtcgcaaa
    16381 aataaaaaga ataagtagaa ataaaaaaag agtttttaaa ctcagattaa atctttgcat
    16441 ataagttcca aaattttgaa tttggtatac taccttctgt tgacaaatcc tccttgattc
    16501 catttacaaa atacatgtta atttctcctt tgtttttggc ttgaatcttg cctctcaatt
    16561 cgcaggagaa aaaatctttt atcaattcat aagtagattg gctaatattg atttttcctg
    16621 gagttccact tgattccata cgacttgcag tatttactgc atctccccaa acatcatagg
    16681 caaacttctt ttctccaata acaccggcaa tgagtggacc agtatggatt cctaatcgta
    16741 attcccaata cggtgccccg atgatttctt taatttcttt catttggttc ataaagtttt
    16801 ggatttcaag tgctgctagt atagcgtcta ttgcatgagt cttattggct tttggaattc
    16861 cacctgcaca catatagcta tcaccaatgg tttttaattt ttctaagcca tacctttcag
    16921 ttactttatc aaattgtcca aaacaagaat ctaattcttt cactagatct actggcgaca
    16981 ttgtttcagc tatctgtgta aagcctttga aatctgtaaa aaggacagtt gcagactcga
    17041 aatagacagg tgctgagaaa cctttttctt ttaattcttt tgcaacttgg cgtggaagta
    17101 tatttaaaag taaacgctct gatttatctc tctccttttc tgcttctagt tgtgccatga
    17161 ctgcaatctt cttttcaatt tcagcttctt ctcttttgga ttctacctct tcgtaaagaa
    17221 aagaattgtt aacagcgata gcaagttgtt cagaaaaaga ttcaatgaat tttatatctt
    17281 gtttattaaa cttctgcatt ccatgaattt tattaatatg taagatacca atcacttcat
    17341 tctgaacaag gagtgggatt tggagaactg atttgatttt taaagcttct gtgatttctc
    17401 tatcgatctc actcatatta tcgatattaa tttgggttac atctctaaga tagaatgtct
    17461 tcttcataaa ataggttaaa tacattgaac ctagttctgg ctttaattct gcgtttagat
    17521 tcttatattt ttgaatcgtt gaatcttcta attcattgct tattaaacat cttgtgaata
    17581 atcttaattt gtctcttgtg actaattgaa gccaaaaaat atttgcatcg aatttttgtt
    17641 ttaaattatc tacagcaaat gagaaaatat tatctaaact tctttctgaa ttgataacct
    17701 ttgcaaattc gtttatgtat tcgatttctt ttctcgcaag gtctgctttt tctttttcca
    17761 cttccgcact tttttttgct tcttccgatt cctttagaat ttgcgcattc gcaatggcac
    17821 cagcaatttg atctgagaaa tttattaatc tttcaatttc ttctggtaaa atggattcat
    17881 ttgtaagttg tttatttata cttaatatac caataacttc tccgtagata actaatggaa
    17941 gtagtaagaa atatttccaa tttgttgttt caagtatcca ttggtcggtt ggagtaaatg
    18001 tatcaaaatc gttaaactca aaaaaataaa attgttggct attgaaagtt tcatagatga
    18061 atcctgtttg ctcattaata ggagctgacc atgattttat agaatcaata gctgactttt
    18121 tcaatgagtt tgatacaaaa gagaaagttc taaattcatt tcgattttta tcaggcaaaa
    18181 tgagccagaa gtctttataa tcaagatctg tttctaaata atacataacg aaactcataa
    18241 tgtctgttag agttgaaact gagttgatat ttttagtaat ttcgttaaga ctttggattt
    18301 cattttttac tctttcgctc tcttctagta gttttgaatt gtagaccgca cctgtgattt
    18361 gatctgcaat cttctttaag ctctcaatct ctgattcaga aagtatcatt tctcttccaa
    18421 aattggatag acctaaaaat ccgataacct cttctttcaa aataagcggt aggcagaaat
    18481 aagatctaaa ttggaaaatt tcaatcagtt ttttttcgaa atctcttttg gattcaacta
    18541 attctttagt gtataaagct tgtttcgtat gaaatacata cccatgcaca tggtctatat
    18601 cgtttaacgg tattcgaata gaataaacaa atttattcag ttcatcaata tcggaattgg
    18661 gaggtaaatt aaaataaagg actttcgcac tcaatcgatc tgcttctacc atgctaagag
    18721 taaacatgtc gatatcatag tttttactta tatgattcat tgtccagtcg ataatggctt
    18781 gaatattatt agaacgattg actgttctag atatttcatt taacgttacc aattctaaag
    18841 ttctttcttc aattttagat tctaagttag aggaaaattt tcttaattcc tcactcaaca
    18901 tctcagattg atggaaagta tttgcaaatc ttctagatag aacagtagct tgaaataaaa
    18961 caaaaattaa tagcccataa ctcgctaatg cagggagttg atttaaacca agcgatctgg
    19021 ttatatcaga aattaaactc acagaaaata aagaaaaacc aagtagaaat aactttgctc
    19081 caactttatt tctaaacgct gcgagtatta atacatagag agtataaaaa ataacaagta
    19141 agataagata ttggttgtag ataagaaaaa atgaataaac tttcattgaa aaaaagattg
    19201 cagagagaga agcaggtatc tcaaaaagac cgataaaaaa aagaattttt tttgagaact
    19261 cacctggata tagactattt ataaataaag cgaaagaaaa tcctccataa taaaaactaa
    19321 taaactctat tcgatgaaaa atggtgaatg gtaaaaatgg aaatgcttca tataaaatga
    19381 gctcgttagt tgaaatggtt cggagagcga ttagaataca aaaaattcca aagtgaaagg
    19441 ctgatgtatc ttttcttctg ctcatatata agcccaagtg gtagagtccc ataatgatta
    19501 gagaactaaa tacaattaaa tctaaagcaa aggttctttt tgctcttgcg tttatctttt
    19561 ctgttgcacc tatttttatc gtagtccacg gtccggcttt gttataatga aaattagaaa
    19621 catgaagtag aatatctaac tcatttcgct ccgttggaat ctcaaatgtt ttgtattgga
    19681 gaaaaggttc tgactcttgc tttgattttc ctactttacc attttgggag ataagttttt
    19741 gatttataaa taaacgatag gcggttcccg tatctggcat tgcaaaggat aaagactgtt
    19801 tggcttctgg aagcaagact tttattttaa aacttgcata gccatattca ggataaccta
    19861 tcctttgcca ctggactgga agatttagat agcttctatt atttctagtt gttgtttcat
    19921 ttggatcgat tagttcttcc cagtaaaact cccattctcc atctagattt gtaatacctt
    19981 cttcaaagtc ttttgcattg aagttccagc ttcttaaatc caagattccc ttcactgctt
    20041 tgggtctctc tacactatcg tctgcataaa taaaattcca aaatcccaag cataggaata
    20101 atacaaagta gataagaaat agattaaatt tactttggga tttcatttat tattacctct
    20161 aggaaaataa gctttgaaca gagcaccttc ttctaggata gacgatactt ctaattttcc
    20221 attgtggaga gaagcaattt tatttgcaat ataaagtcct aatcctgcac caattacatc
    20281 atagcttata aaactattga ttcgaaagaa cttatcaaaa attttctttt gatgatcctg
    20341 cgatattcct attccagtat cttgaatcga aataataata taatcagctc gaatttcttc
    20401 ttttatataa acagatccga aatcggtatt atattgaatg gagtttttta aaatttcata
    20461 aaataacttt tctaataatt ttctgtctgc atttattttt aatgcatatg aaacgtggtt
    20521 ttcaatttta atttttttag gctcagccaa ttcgattagg tttttaattt ctgcatctag
    20581 aatttcttta acagaacagt attctagatt tagtttaata ttcgactcta aatctgttgt
    20641 cagaattaaa tcttcaacat atcgattcaa aatctttgca tttgtgaata ttgatttacc
    20701 tagtttagtt ggattaattt gatctgtttg ggtaattagt atttctgaat cagaataaat
    20761 attctcaagg ggctttttta attcttcaga taggtttgat aaaaattgat ctttagtgtt
    20821 tgtaagaagt tcaatttcaa ttcttctttt ttctgcaagg tttctctgct ctattgcaat
    20881 tgctctagat atttcacttt gtttaaacgc tttcgaaaat ttagacgata gtagataggc
    20941 ttgagagaaa ataaaaacta aaactccaaa ataaataaaa tggccagtat ttagaattcc
    21001 tttagtatat aaaatatcgt tgatcgaaaa aatagagaga gctaaaattc ccacacaaaa
    21061 ggaaactgcg ccttctcttt tatatatgat tgctttcgaa atggaaacca gtccatggag
    21121 taatgctgta attgtaagaa tataaaaagc agacaaggtt gagtatgaaa attggatcgg
    21181 ggtgagtaaa aatattaaat taaagactaa taaaatataa ctataaacat tgaatgcttt
    21241 agttttaaat tcatttggaa ataattcttt caaaaaggat aaaatataga aaaaagcagt
    21301 gaatagtaaa atataatcca atttatgtaa aatattctcg tcgatatacg gaaagacagc
    21361 ggctaatatt ctttctcccc taaaaaaagg tcgaacagaa aagaataaac acaggaatgc
    21421 aaaataaata gcagacttat cactccttct cagtaaataa ataataaaat ggagtatgct
    21481 cattataaaa agagccccga aaagaaaaaa atctagcaga attctattta gcttttcttc
    21541 ttttaatgtt tttgtttttc caataaaaaa aggatttttt aatcctccat ctaggtgaaa
    21601 aaaattagaa acatgaaata gaatttctac tttttcacca gatggtattt ttagtaggac
    21661 aggttttatt ttgaagtcag attcttcttt tgatttgcct actcttcccg attgatcaac
    21721 taattctccg tttatataaa ttgcatagct agaatggatt ccatttgtat tgatagcgag
    21781 atcattgaat ttattattga gaatagtaag acgataggtg gcatatccat tggatccaat
    21841 tttgttatta gaggataaag aatcattcca gcgatgagat gctgagatag aattcaactt
    21901 atgatgagaa atgtccttct gatgtataaa ttcattccaa taaaattcaa aatcaccgct
    21961 tatttccagg gatttattct gattaaaatt atgagattga agatctaaaa ctccgttctc
    22021 catccttaga atttctttat tttcattaga acaagagata gagaataaaa aagcgaaaac
    22081 gatcaagaac ccattgatcg atttgattga gaacatagtt ttcaatcgcc tagaaatttc
    22141 tttatttcta gaacaatttg acctggtgaa aaaggctttg tgatgaaact gctagctcct
    22201 gcgtcagccg ctgtttttga atccgttgtt cctcccttgg cagttattac gaagattggt
    22261 gttgcggcaa attctggtaa gtcccttaat tcttttaaaa gatctaatcc cgttttcaca
    22321 ggcatcatga tatctgttat aattaaatct ggtttaattt cctttgcttt gtctattcct
    22381 atttgtccgt tatttgctgt aattacgtca taattttctt ttttaaaaat atctgccaag
    22441 atttttgcca taggcgcttg gtcttctacg atcaatattt tcttcatatt ttagagtcct
    22501 catttctttt cggtaaaaat atagtaaatg ttgtgcctag attcttacta gttttatagg
    22561 aaatcgttcc gttatgttcc tcgatgattt tttttgacat ataaagacct aacccatttc
    22621 ctttggcttt accctcagtt gcaaatactt caaagatttt ttcttcaatg ctattaggca
    22681 atccaggtcc attgtcttct aaatgaagat gaatgcctgt atttaaattt tcagatttta
    22741 tctttaaaaa atattctttt tttgaatcat gcatagcttc tcttgcgtta tttgctatat
    22801 taattataac tcgcctgatt ctttctttat caaacaaaat tctttcatat gaattgcatt
    22861 caatggataa attgatccct gcatactgaa agtctattat taaaaaatca tataactccc
    22921 ttaagaattc gcatagtgaa tgcggatata aatttagtct gaaattccct ttactaaaat
    22981 ctaagatttc atacgtcatg tcactgagtc tgtctatttc tctaagtatt aacttcaagt
    23041 attcagattt cttggaagga tcgatgtgaa cactcattgt taagtcagca tatgctttga
    23101 ttgttgtcat tggatttttt atatcatgaa ctataccaga tgccatttct cctattaccg
    23161 aaagtctttt tcttgattcc gctaaggtct ccatttcttt aagtgccttt tgtaattcag
    23221 aggttctatc aattactttt gattcaagac tttcatttag ctcgatcgtt tctttatata
    23281 gagagtttaa tttttgaatg atgttgttta gatggattgc ttgcagtccg aaatcatcgg
    23341 aagaggttgg catactcacg attgagaaat ttccttttcc gagttgatcc aaatgtgtat
    23401 tgacagagga aattccattt ctaaaagtct tggcaacaac gtatcctgtg actaaaactg
    23461 gaattggaaa caaaacactc atggttataa tatgcagtgt gatattttct aatcgaatta
    23521 attgtgaaat agatccatat aacatataac ctagaacaat aattggcatc gttataatcg
    23581 ctatgagtgt aaaaagaatt ctctgaaagg aattaaattg aaaaatatgt tctttttgaa
    23641 tttgtattct tttaattttt cttaattgga aaactttctt taactcattt tctgtaataa
    23701 aaaaataagc gatataggaa atagggctga ccatcagaaa aagaaatgga gctgttagat
    23761 ggagagccgg tcttatgtca tttaaaatca atatggctac taggtgagaa ccgatcactc
    23821 ctgtcatcca acgaatcact atcatttttc cttctctaaa aggatatctt agaattgcta
    23881 gttttaaact aacttgtttc tcttttggga cattttcaga tttgtagatc tctaaaattt
    23941 gtgaaaaaat tttttttaag gaaataaaac gccagagtgt tccaagaatg accattaaaa
    24001 tcgcatttac aataccagca gtcgcaacat atttatactc ttcaactgcc aaataggaac
    24061 cggatccact cacaacgcta aaataaattc cgaaaggaag cggaactata tgtgtaaaag
    24121 tttctaatag aagagttagt ttcaaaaaga atcttataaa catagaattt agagcttttt
    24181 tatgaattat tgggagttga tctctcaaga gtttctacaa gaaatgctca ttctaatttt
    24241 ggggtcaatc aaaaaagaaa agactataaa tttctaggga attcagaatt aaaatgattc
    24301 ctgattgact tgtaaaataa agaatcaaaa gtatttatca tgaaattgtt ccactcgatt
    24361 ctttatttac ttattgttct tattttttca aactgcaaac agagtctcag ctctatttca
    24421 gatattccaa tagaatggga atacgcagat tcagagcaat atgagccttt gagttttcta
    24481 ggagataaag aatttaaagc gatagacgct aataaagttc acttactttc acaatttgtt
    24541 cccaatgaaa agggatatat ctggttacgt ggatttttta attataattc ttctaatgaa
    24601 ccaatttcca ttttaatgga aaaaattgtt tgggctgagc aagtctattt aaatggtcat
    24661 tatttaggag gaacgattca gagagaaaag gaacaatgga attattggaa cttatataga
    24721 aattacccaa ttgcaaaaga tagtttaata gacggaaaaa atgaaatttt acttagagtc
    24781 tatgtagaat cagaaggatc tgttccagat cgaatcatgg ttggtcctcg tgtagcgata
    24841 gacgaattag cttttgttaa aatgttttat gaaagctact taaatggaat agtaactgtt
    24901 ttatttattg taatagcttt atatcattcc ctgatctatt ggaaaagaaa acaggatgta
    24961 gaaaatcttt attacgctat attttgtttt acatactctc tctatggact caattttgtt
    25021 gggtggattc taggtttatt tctgaaggtt aattattttt attttcaaaa atttattttt
    25081 ctatccatgt atatttctgc atttgctctt taccggttta ttgccatttt cctcaagaga
    25141 aaagatcgaa agtggttcaa ttacctttat atgatactca tagcgcttcc gattctgctt
    25201 ataattgctt ctcctaatta ttctttcatg tataaaactc gcggttacat aatgttcttt
    25261 gtaattccgt ttctattgta tatactttta gttccaattt ataattatat aaaatacaag
    25321 aatgcagaag caaagactat gcttgccagt ttagtgatta ttttttcagt agcactgcat
    25381 gatatattaa atgttatatt taagtggaat tccttttttt ggttaggacc tgggattcct
    25441 ctttttatgg gaagtatcat gtttctactt ggcaataaat ttgtagatgt ttacaaccaa
    25501 acagatgagt tgaatgaaac gttagagcaa aaggtagtgg aaagaacaaa agaagtaatg
    25561 gagaagatgg atgtaatcaa ggctctcaac attcaacaag atggagatta ttacttaacg
    25621 tctctgattg aaagacctct tggcacaaat tataataaat caaagaatgt aaaaactgat
    25681 ttttatgtag aacagaaaaa aagatttcaa ttcaaaaata gaaattcaga attaggtggt
    25741 gatatttgca taagtggaaa tttacgtttt ggagatggaa aagatcgtca tgtattcttt
    25801 tttaatggag atgcaatggg taaatctatg caaggggctg ggggagcgat agttgcaggc
    25861 actgtaatta ataacattct agctcggtct gccagaaata ataaaatcca aaaaattact
    25921 ccagcggaat gggtaacaga aacctattta gaattagata gtgtatttaa aacctttgat
    25981 ggatctatgc tcatgtcttg ttcttgtggt cttgtaaatg agaggacagg gcaaatgtgg
    26041 tatttcaatg cagagcatcc ttgggcggtt atctatcgag atgggaaagc ggaatttata
    26101 gaatcaggtt taaatttaag aaaactggga actataatag atggcaatgt atttaacata
    26161 caggaatacc agctttattc gggtgatata ctatttgtgg gatctgacgg tagagatgat
    26221 ttagatatat cgcataacgc tgaaagagaa attaatgaag atgaaactag atttttgaga
    26281 gcagttgaaa aatcgggggg aaatcttact aaattagtgg aagcaattca ttctattggt
    26341 aaattaacag atgatttatc cttgatccga attgaatttt tagcaaagca ggactcagac
    26401 actgtagtta agaatctatt cgaagaaggt aagtcactca ttgaagcagg taatgtttca
    26461 gaaggacttg ccaagttact tagtcattta gaattaaaac cagatgattt agcaaatttg
    26521 gaattgatag caaatactta ttatcatatt aaagattacc aagaagcaag tttttatatg
    26581 gagaaaattt tagaaatgac tccaacggat acagatacaa ttttcaattt atccttatgt
    26641 cataagcata tgaaagatat tgaggtatct actcagtatg cagaacaact gtttcagcta
    26701 aatccaaaga aaatttccaa tgtaatcaat ctagcagaca attatagaat tctaggcaat
    26761 ctagaaaagt cacgtgaaac tttaaatctt gccattgatt ccatttctta ttttgaaaat
    26821 gctatcaaat tagataggtt attaaaagca aaaggctatt aatattcctt gactaaataa
    26881 ctaagtctca ttcaatgaag taataaattt caaaatggag gaaacgttat gaataggtgt
    26941 aacaaccatt taccgttcca atatatttta gatcgatgcc aggggctaat tctaattagc
    27001 tgtctattga ttactacgaa ccttttttca gcagaaatgc tagcagttgc tatggcaaaa
    27061 gtcggaaatg ctgtctatga gagaaataat aagcaagcac aaatcaccaa aggaatgatt
    27121 tttgaaacca aagacaaggt gattacaaca aacggaatag ttgatattca attggggcaa
    27181 agctctatta tccgtgtagg aaaaaattca gttgttttga tatctaaatt aattgaagaa
    27241 aatggatctg agaaaacaga attgaatcta aataagggaa ttatattctc aaagattagt
    27301 aaaaaattag at
//
LOCUS       DAOQOS010000076        26476 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_846_length_26476_cov_6.017713, whole genome shotgun sequence.
ACCESSION   DAOQOS010000076 DAOQOS010000000
VERSION     DAOQOS010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 26476)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26476)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26476
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_846_length_26476_cov_6.017713"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..488)
                     /locus_tag="PLF66_16045"
     CDS             complement(<1..488)
                     /locus_tag="PLF66_16045"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HMZ65606.1"
                     /translation="MKENSKSQFEELYQKYERYIHNLLFKFTRDIDTTEDLKQESFLR
                     IFQSFDSFDPEKGSFSAWASTIARNIFFRYVENSKKNANTVYSEEIISQIGDNSLNPE
                     FTFQKKNIREEIKNLISCLSEPEKSIVIFKYQNNLTLDEIAAKLAISRRTVSRKYITA
                     MT"
     gene            complement(611..2350)
                     /locus_tag="PLF66_16050"
     CDS             complement(611..2350)
                     /locus_tag="PLF66_16050"
                     /inference="COORDINATES: protein motif:HMM:NF017183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HMZ65607.1"
                     /translation="MSLRLILFPILFVSLFNGCLKAKKSPFDASNTSNPVFAINVLNI
                     KPGSISSGASNSTSSAPSNLTYSLSPLQSKLIPGNPITITPTITGTVDSWEITPQPPS
                     WMTFDTKTGVITCNPPAGLPAASYSFTVTATNAGGKVSFNVPLQLLGSGDPVWQVING
                     VTGGNTYAGVNSMKYDSNCNCLYIAGTTTGNLDGQTIPTTGGARSGFLSKYDLNGNKL
                     WTKVFGVSGSSSINTNGITVDSSGNIYITGGVESGNFNGLTISVGASPNVAGYIIKYD
                     PAGNVVWTASSAPNVRHYYSGIVIDINSDIVVAGAVYASSIDGMTNTGWADQAVIAQK
                     FDKTTGARINGAIVGGSSSLGTDGYGVGSDSSGNIYVAGASRAPSPNCGIGSTAYWRP
                     VLFRFNSSMSYIGCTFITVSSTAFAFGATASPNGESYISGYGSGTIDSIGPIGTTDGY
                     FTKFDNLGNKLWTKRLGVPGAITTINSVVYEPNVNMIYITGLTNGNLAGTVTGTRDMF
                     VAKYDPSGNQIWIKMQGINNDSIGLGVGDGTSIVFDQSKTMYSFGHTNGTVNGITNPA
                     APNNALFLVRNVQ"
     gene            complement(2453..4216)
                     /locus_tag="PLF66_16055"
     CDS             complement(2453..4216)
                     /locus_tag="PLF66_16055"
                     /inference="COORDINATES: protein motif:HMM:NF017183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HMZ65608.1"
                     /translation="MKRIAISQIFFCISLLGCLKAEKSPFDASNTSNPVFAVSVFNIK
                     PTILGGGTTPNAITPPSSVSYGSLSSFKMIPGVAVNFTPTVTGTVDSWNINPTLPTGL
                     TFDSKTGSISGTPSASYLSGGFALTTFTIQAVNAFGSTEFKIDLQILGTGENVWTVIH
                     GVTGYATTAGYGSLFFNSNSNSLYSAGATLGALDGESNPSPGVASGFVTKYDLNGNRI
                     WTKLSIATSGGFGQNGLSVDSAENIYIGGSVYGPTTFDGITTPNSYNAAISKFAPDGT
                     RQWSSLRYQNSSESFGNAIYADASGSVFLLGQAKGGLDFSANTAGSDAANSVMKYNSA
                     GTWQSTTLVGTSSASTGRHVGVYAGVTDSAGNLWTGGYSQAGDKCANPDLKRTATIFK
                     YNSSVVYQACYSLVSGTGGTAGEVYIFGITRDSSDNLYVTGYTNRNLDGITKTSTAGD
                     SYYDAFIAKFNSSGTKLWTRLLGVTGNTTTKAYAITKSVDNFYYIIGETNGNLGGQTL
                     NGAIDLFVAKYDTNGNLQTNGAQPWVKLLGSSGSTTVGTGIAFDTNNTMYATGHSTGD
                     IGGTTNPVKPNNAHLLVRFVQ"
     gene            complement(4631..6643)
                     /locus_tag="PLF66_16060"
     CDS             complement(4631..6643)
                     /locus_tag="PLF66_16060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010410559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HMZ65609.1"
                     /translation="MSLTEDHDIQSIFSKKIGRKWSEFELREEQIKMSKAVEDAFQSG
                     EHLLVEAGTGVGKSLAYLLPAAVYSLENQVPVVISTETIALQNQLIAKDIPIVAKILG
                     KKITAEIALGANNYVCKRKLQSVISNGSFPIEMGNHLKPFYEWESETETGIKSEYTGF
                     ATNEFWNQITRDADNCLGRRCPNFSESYYFLEKDKWNKANILIVNHYLLAANIAGEFK
                     ILPEFKNLIIDEAHSFPDILGRSFGLKTSYEDINKIIQFVGGTERRQGLIHKLQDSTI
                     RDKCLEVISNINKSIILFFNKLYSEVPMSFNAQRIQKKFALDDGVLEGLLFILADKLE
                     AAKKSYNKDSDDLQDKEMILDLEMSIKKLNETGEVIEQFRNNKDKEMVVWVEPPEERK
                     NEKFLKISMQPMYPEKIIEEKLIPRLENMILTSATLSSGKKDFKFFKNKIGKIEAGEI
                     LLASPFDYEKHCLIYLPKNVRDPATEPDEYHDDISKLIPMLLDLTEGNAFVLFTSFKS
                     LQTVYDAIVDTCKYPIFSQKELGADKAKAEYLNTENSVLFGVSTFWQGIDIKGDKLKS
                     VIITKLPFQPPNEPVLEARMDEIKKKNGNPFGEMQLPQAILTLKQGFGRLIRSKTDTG
                     IVSILDPRMQTKNYGKQILDALPPAKKVYSLKDLKIEYKKIIMGNL"
     gene            complement(7018..8214)
                     /locus_tag="PLF66_16065"
     CDS             complement(7018..8214)
                     /locus_tag="PLF66_16065"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HMZ65610.1"
                     /translation="MKLRINQIKAGTILDGKICYEDGKTLFANRTVFTKDILKKLESE
                     KVEWVEFYPLQEKTKKNDQDKKEEEKIEKILDGFHYGGITKEVIKESISSFRSITFNL
                     LNGNDSIDFTSCKNSINAVYDQIKTNPSAIINLLDIKNHDDYTFCHSINVGIISLALA
                     QKMGYPESEVQMIGLGGFLHDIGKIAIPASLINKTTVLSEEEKRIMKSHPSHSYRIMK
                     LDKNLNRRIVNMAYEHHERYDGKGYPLGISGDKLDDYSVIVALADVYDALTTVRSYKP
                     AFSPEESIQVIETYTGTHFAPRIAEKFIADIQNSLAAKTQLTKGSLVLLSTNELAQII
                     HYKTNPDGADIIVQLLTDGKNSRLRNPIRINIKGDKVRTIKRGVKTQELDGDSLRVNL
                     TKSTSL"
     gene            8419..8490
                     /locus_tag="PLF66_16070"
     tRNA            8419..8490
                     /locus_tag="PLF66_16070"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8451..8453,aa:Thr,seq:ggt)
     gene            8506..8579
                     /locus_tag="PLF66_16075"
     tRNA            8506..8579
                     /locus_tag="PLF66_16075"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8538..8540,aa:Trp,seq:cca)
     gene            8608..8796
                     /gene="secE"
                     /locus_tag="PLF66_16080"
     CDS             8608..8796
                     /gene="secE"
                     /locus_tag="PLF66_16080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000869352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HMZ65611.1"
                     /translation="MKTLTFLQECRAELQKVQWPSREEVINSTMVVLGTVIVFSLFLF
                     ISDSLFVRLLKWFWSLSG"
     gene            8803..9339
                     /gene="nusG"
                     /locus_tag="PLF66_16085"
     CDS             8803..9339
                     /gene="nusG"
                     /locus_tag="PLF66_16085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HMZ65612.1"
                     /translation="MMAIKWYALQVYSGHENKVKTNIEKMVAQHSLENKITQIKIPTM
                     EVAEMRNGKKTITKKKLMPGYVLIEMEMDPDTQFMVQNLPSVSTFVGNKAPEPLTLEE
                     IKNLFNEMGDVKSEETAKPRLLFNVGETLKIVDGPFANFSGVVDEIFPDKGRLRVKVE
                     IFGRSTPVELDFLQVGKS"
     gene            9370..9798
                     /gene="rplK"
                     /locus_tag="PLF66_16090"
     CDS             9370..9798
                     /gene="rplK"
                     /locus_tag="PLF66_16090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010414651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HMZ65613.1"
                     /translation="MAAKKFVKQIKLQVEAGKANPAPPVGPALGQAGLNIMEFCKQFN
                     ERTKGQIGMKLPVVISVFSDKSFTFVTKSPPAALLVKKALGIESGSGTPHVTKVGTIT
                     RKQLEEIAKTKQADLNANDLNAAVNIIAGTCRSMGVTVAG"
     gene            9810..10502
                     /gene="rplA"
                     /locus_tag="PLF66_16095"
     CDS             9810..10502
                     /gene="rplA"
                     /locus_tag="PLF66_16095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002999964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HMZ65614.1"
                     /translation="MKRGKKYKALKDKVNREKLYNLADAVGLAKETSYTKFDGSIEIA
                     TQVNYKSLQNIRGTVSLPHGTGKTVRVLVFCKGDKQTEAKNAGAEFVGDMDLIAKVAE
                     GWTDFDACVATPDMMKEVGKLGPVLGRKGLMPKPKAGTVTTDVAKAVNELKSGKVEYR
                     PDKGGVIHLGVGKVSFDKQKLVENIQAVVATLFKDRPSDAKGDYLKNFAVSATMGIGV
                     KVDAKELANSVN"
     gene            10518..11042
                     /gene="rplJ"
                     /locus_tag="PLF66_16100"
     CDS             10518..11042
                     /gene="rplJ"
                     /locus_tag="PLF66_16100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010573744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HMZ65615.1"
                     /translation="MANQKNIETVAELKTKLEAKPNFILAAYSGLTVKNMTDLRVSLL
                     KEGSEMKVLKNNLFLRALKESKSHSDKQIDFGAEYKGPIAAIFSKENLPTVAKICKDF
                     AKTNENLVMKGGFFDGTVLDVKGVEGIAGLPSREELLTQIAFGMNNVMSTLARAINAV
                     AEKNAGGAAAPAAE"
     gene            11125..11499
                     /gene="rplL"
                     /locus_tag="PLF66_16105"
     CDS             11125..11499
                     /gene="rplL"
                     /locus_tag="PLF66_16105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002775053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HMZ65616.1"
                     /translation="MSTESLLEQIGNLTLVQAAELVKAMEAKFGISAAAPVMVAGGGG
                     GAAAAAAEEPTEVSVILKAHGDKKIDVIKAVREITGLGLKEAKDLVEAGGKPVKEGIS
                     PDEANTIKKKLEEVGAQVEIKK"
     gene            11681..15358
                     /gene="rpoB"
                     /locus_tag="PLF66_16110"
     CDS             11681..15358
                     /gene="rpoB"
                     /locus_tag="PLF66_16110"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002973774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HMZ65617.1"
                     /translation="MITQTERKRVNFGKITNLDFLPNLIQIQKKSFDWFLQADVKDPT
                     KRKNQGLEAVFQETFPIESPNGDVIMEYSHYILGEKRRDQQECKDTDSSYAIPLKAVI
                     RLIIKETGEIREQVVYMGDLPIMTDQGTFIINGAERVVVSQLHRSPGIFFAYDQIKSA
                     YSARVIPYRGSWLEFEIDNKGILVAKIDRKKKFPATLLLKALKVGLNEDVLKLFYTSS
                     KVKIYGASPKELKKVIGRRTISDIINFETGEVMLEAGAKINEDNIDILKEMKVKEVNV
                     IDYPNGKDNSIIINCLEKDGVNDYEEAIKKLHMIMRPGEPSTIENAEAELKRLFFSPK
                     TFDLGKVGRYKINSKFEFHKPKEFTNCDNRTLRPEDIIETVRYLVMLMSEVENYYHDD
                     IDHLGNRRVRSVGELLSNQLKLGFSRVERVIKERMTVQEIEQQTPQLLISIKPVTAVV
                     NEFFGSSQLSQFMDQTNPLAELTHKRRLNALGPGGLSRDRAGFEVRDVHYSHYGRMCP
                     IETPEGPNIGLILSMSSFARVNDYGFLETPYRTVKSGKVQKQIEFLTADKEEYRYIAQ
                     ASAAIDEKGDFKNKLISTRHRSDFPFRNPNEIQYMDLAPMQVVSVSTALIPFLEHDDA
                     NRALMGSNMQRQAVPLMVEQAPYVGTGMEGRAAYDSRICIIAKKSGIVVKVDSKAISV
                     REEGAKELTVYPLIKYKKTNQGTCFNQKPVVSVIMAEKDGKVVKISKDKMEVQYGEEN
                     ATYSLLYGTKEFAPLVREGASVSKGETLAGQVVTGEKIDDQGNILVKGTILADGPAID
                     NGYLALGKNVLVGFMPWEGYNFEDAIIISEKIVKDDIFTSIHIEEFEIQARETKLGQE
                     QITRDIPNLSDKAFRDLDETGVIRIGAEVKAGDILVGMVTPKGETDLTPEYKLLHSIF
                     GEKAKEVRDSSLRLPNGNEGTVIDIKRYYRERGDDLAAGVEELVKVYVARKRKLLVGD
                     KMAGRHGNKGVVARVVAEEDMPYMKDGTPLDIVLNPLGVPSRMNLGQIFETQLGFIAD
                     KLGINFETPVFDGANETDIEEYAKEASAPNNSKFQLFDGRTGEPFLNEVFCGHIYMLK
                     LAHLVDDKIHARSTGPYSLVTQQPLGGKAQFGGQRLGEMEVWALEAYGASHTLQELLT
                     IKSDDMLGRARIYEAIVKGINSIRPGIPESFNVLVQELRGLALDIIITDTEGSTVDIA
                     DYEDEYSRNKKKIKIESIENI"
     gene            15368..19627
                     /gene="rpoC"
                     /locus_tag="PLF66_16115"
     CDS             15368..19627
                     /gene="rpoC"
                     /locus_tag="PLF66_16115"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004783957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HMZ65618.1"
                     /translation="MKNYTSFDSITIKIASPERIKEWSYGEVKKPETINYRTLKPERE
                     GLFCEKIFGTTKDWECYCGKFKSIRYKGVICDKCGVEVTHSKVRRERMGHIELAAPVS
                     HIWYYRSVPSRMGLLLDMTMNALKTILYFEKYVIIDPADSGRLKGELLDEDEYHSYLD
                     EYGDKFIAGIGGDAIKELLARIDVDAEARVIRQKIQEKKVTDKRLIKRLEVLEAFRDS
                     GNRPEWMVLDNVPVIPPELRPMVQLDGGRFATSDLNDLYRRVINRNNRLKRLLTLKAP
                     EIIVRNEKRMLQEAVDALFDNSRRKRTVKGKGNRPLKSISDMLKGKQGRFRQNLLGKR
                     VDYSGRSVIVVGPELKFHQMGLPKKMALELFKPFIMKRLVDLELAPNIKSAKKKVEAE
                     DKDVFEALETVVREHPVLLNRAPTLHRLGIQAFLPVLVEGKAIKLHPLVCHAFNADFD
                     GDQMAIHVPLSPKAQLEVWMLMLSPHNILNPANGHPICGPTQDIVLGIYYLTSEVPNA
                     KGHGKFFTNLDEIQYALDGGWLDLRAKISVLYNEKIIETTPGRMIFNTAMPKGYNFVN
                     RVLGDKETNKIISEVYDTFGPAATVVMLDDIKKLGYRYATIFAPTISIEDIRVSPQKQ
                     ILVSEANKEVEKADMEYRKGIITNEERKKKVIEIWTKANDRITDSMFKELEKDRGGFN
                     PVYVMAASGARGSKQQIRQLAGMRGLMAKPSGEIIELAIRSNFREGLGILEFFISTHG
                     ARKGLSDTALKTADAGYLTRRLVDISQDVIINEVDCGTDQSVELSIVKEGENVIVSLA
                     DRVFGRYTAEEVIDPVTDKVVYPKNTLIVRSVGQKIENLGYTKIKVRSPLTCDAKHGI
                     CVKCYGMDMARLVPVEIGEAVGTIAAQSIGQPGTQLTMRTFHIGGAASAKVKEKEHKV
                     SFQSVVKLINGRLIQNQKGQTVFSRRGSIVVQHLIQDLQLSDLQNIRVNNGQNVLKGE
                     MISTNSKGEMIHAEMPGRIEISGDRFRIIGEDIVIPVKIGTILLVKENDIVKANSPIA
                     EFDPFNDLVISEVEGTVTWVDMEVGKNIRRDEDVKTSNVIYKVIEQKREKLNPRMVVS
                     NGDTTEEYAVPVDAIITIVTDDKVKIGDILFKIPSIAVKTRDITGGLPRVDELFEARR
                     PKDAATLAETSGRIEDRGEIVKEKRVFYIIPDNPDMERIKVTIPLAKQLRVQDGDYVK
                     QGDQLDDGNFDPHDILRVRGTNALHAYLVKEVQEVYRLQGVHINDKHIEVVVRQMLRK
                     VMITDSGDTSFVSQQQVDRFTFREENKRVDAEGGSPSQATPILLGLTKASLNTESFFS
                     AASFQETTKVLTDAAIKGKTDQLIGLKENVIIGHMIPAGTGMRKYQEIDVFKDFYGDL
                     DKMTAEEEEEKFPQMQTNSLAVQITDKNDLESDVDDEEDDE"
     gene            19715..20089
                     /gene="rpsL"
                     /locus_tag="PLF66_16120"
     CDS             19715..20089
                     /gene="rpsL"
                     /locus_tag="PLF66_16120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HMZ65619.1"
                     /translation="MPTINQLIRHGRNKQKKKTKSPALKSCPQRRGVCTRVMTFTPKK
                     PNSALRKVARVRLTTGIEVTAYIPGEGHNLQEHNVVLIRGGRVKDLPGVRYHIIRGTI
                     DTLGVDKRRNGRSKYGAKRPKA"
     gene            20099..20572
                     /gene="rpsG"
                     /locus_tag="PLF66_16125"
     CDS             20099..20572
                     /gene="rpsG"
                     /locus_tag="PLF66_16125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020770512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HMZ65620.1"
                     /translation="MSRRRTKAEAKNIVPDIKYNDKVIGKFINCLMFDGKKSVAERIF
                     YDAMDNIQSKLSSDPYKVFHEALNNVKPEVEVKSRRVGGVTYQVPIEVRPERRLALGI
                     KWIIRYSRERNEKSMSSKLAAEILDAHKGTGSSIKKKEDIRKMAEANKAFSHYRW"
     gene            20723..21292
                     /locus_tag="PLF66_16130"
     CDS             20723..21292
                     /locus_tag="PLF66_16130"
                     /inference="COORDINATES: protein motif:HMM:NF017496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HMZ65621.1"
                     /translation="MASHILEREFLRENFIPISVEAYHYLAEKNLISEKAELIEGVIV
                     KKLPKSPIHAALVRKLIHFFQKSISNQFTISSENPLIFSHSVPEPDLAIVEFREDFYA
                     KEHPSFAHLVIEIALSTNAFDRDKADIYASAKIPEYWIFNLQNKTLEVFQNPDQDKYQ
                     TTSVLSASDSIHPLFQNEILLDLKEFLSF"
     gene            21612..21950
                     /locus_tag="PLF66_16135"
     CDS             21612..21950
                     /locus_tag="PLF66_16135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65622.1"
                     /translation="MDPMIVHDKTFGAGIQVYSKDKRMEALFSPEDEIIKINSCEIKI
                     ADKISYEESNNAVIGDDFLVDELLHFQSMFSDLEPDEKIFLTTIRNKDTIVIEFSEKL
                     LQATANSLKK"
     gene            22392..23660
                     /locus_tag="PLF66_16140"
     CDS             22392..23660
                     /locus_tag="PLF66_16140"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010573961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HMZ65623.1"
                     /translation="MAKNQRVVITGIGTVLPNASTVDSFWENLRDGKSQIGYLTRFPT
                     DNFSIKVGAELNDFDYKKYLPDLDPKLAKNYNHETLVLMAAMDLAQKNAKLAKDSLDP
                     TRVGFIDSSSRSSLAWWEHAWKRYTELKDESVFDRYAVLTSMASNPTSLTAINSNIQG
                     FVTTISSACVGGHHAIALCFQAIRKGRADVMYAGGHEFPLIKPLMQMYSDPQSRVMSL
                     EKEDPKNAIRPYDIKRDGFALGEGAAVLCLESYDHAMERGAKIYAEILGTLSYNEADH
                     AMRMDITGKKACSGIKKLIQISKRSLEDVDYFCGHGTATYTNDLAEGRSMKLLYDGVP
                     KNKWAPLGSIKPIYGHTFGAAGILNVAATAMMIEHQTLCPTINLKNVDPECDQDHISE
                     GPRKTKLDFAISLAFAIGSQSSFVSLGVPD"
     gene            23873..24835
                     /locus_tag="PLF66_16145"
     CDS             23873..24835
                     /locus_tag="PLF66_16145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016756539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HMZ65624.1"
                     /translation="MKLKSNGIQITGYGDYYPEKILTDEEIRGRLKYPDMHPAEKAVI
                     GDIGVTQRHRSSEKETSVYMASKACEMAMKNAGVSPESIDLYLFCNWTERYYLPDLAP
                     QASLLNGTKNALAFDLGTACTGFVHGVQTASCYLQTGKWKKALVVGSERFSERTKKGG
                     YGEFTAGDAAAAVVMEYTGNTETGLVDTCLYDVGTLVDVITCTAPAGLIRSYPDLVTN
                     AADYSLKAIDLLMERNGLKESDITWVVPHPGTDIVVKDILKRTKIPKEKYLMNYPKMG
                     NVSAASIPTVLAENYNKGIIKKGDVVLTPAVGGGFYWGGILYIA"
     gene            25296..>26476
                     /locus_tag="PLF66_16150"
     CDS             25296..>26476
                     /locus_tag="PLF66_16150"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HMZ65625.1"
                     /translation="MQLIEGYEIKERINTNSDIEVYRAVDKADNKPIILKSIPIHNEF
                     HASIINLKNEFDVMKYLSSDLMLKVYAFQRYSNGFFLAYEDTGGISLKEYTQGKKIPV
                     DKFYDIAIRLAEILSEIHRKKVIHKDIKPENIIINPNNMDVKLIDFGISTRLSKEETK
                     WTAANVLEGSIQYISPEQTGRMNRSVDYRSDFYSLGVTFYQLLTGKLPFENTDLLELV
                     HAHLAKTPLTAHELDSSIPVSLSNIVDKLMAKTAENRYQTALGLKYDLELAKEAKPDQ
                     SIVIGSKDLSDEFKIPQKLYGRSDDILKLIETFAEIHENGASKLMLISGYSGVGKSSL
                     VKEINKPITASRGYFLTGKYDQYNRNLPFSAIIQVFTNLIRLFLTESPESIDTWKSKI
                     LT"
BASE COUNT         8802 a         4394 c         5118 g         8162 t
ORIGIN      
        1 tttgtcatag ccgtaatata tttacgactt actgttcttc ttgaaatagc aagctttgct
       61 gcaatttcat cgagtgttaa attattttgg tatttgaata taactatact tttttcaggt
      121 tcagaaagac aggaaattag attttttatt tcttccctaa tatttttttt ctgaaaggta
      181 aactctggat ttaaggaatt gtctcctatt tgggaaatga tttcttccga ataaacagta
      241 tttgcatttt tcttagagtt ttctacatac ctaaaaaaaa tatttcgagc tatcgtggaa
      301 gcccatgcgg aaaaacttcc tttctcagga tcaaaggaat caaaagattg aaaaattcgt
      361 aaaaaggatt cttgctttaa atcctcagtc gtatctatat ctcgtgtaaa tttgaaaagt
      421 agattgtgaa tatatctctc atacttctga tacaattcct caaattgact tttagaattt
      481 tctttcatcg aatcgtttta aaagcaggtt gttctaaatc tatttcttgt cgagtggaaa
      541 tatcagtctg tcacggctta tatattttat gatgaaattt tagtggaagt agttttaaaa
      601 tatatctaag ttactgcaca tttcgaacta gaaataaggc attattagga gctgcagggt
      661 tagttatacc gttaactgtt ccattggtat gcccaaatga atacatggtt ttgctttgat
      721 cgaatactat tgatgtacca tcgcccactc ccaaaccaat actatcatta tttatccctt
      781 gcattttaat ccatatttga tttccagatg ggtcatattt ggcaacaaac atatcacgtg
      841 ttcccgtaac tgttccggct agatttccat ttgttaaccc tgttatgtat atcatattta
      901 catttggttc atataccacg gaattgattg tagtaattgc ccctggaaca cccagtcgct
      961 ttgtccataa tttattccct agattgtcaa attttgtaaa atacccatct gttgttccaa
     1021 tgggtccaat tgaatcaatt gtgccacttc catatccgga gatataagat tctccattcg
     1081 gggaagctgt agctccaaat gcgaaagcag ttgatgatac ggttataaat gtgcaaccaa
     1141 tatatgacat acttgaattg aatctaaata gaactggtct ccaatatgcc gttgagccta
     1201 tcccacagtt tggtgatggt gctctactag ctccagcaac ataaatattt ccggaactat
     1261 cgcttcctac accataaccg tcagttccaa gcgaactact tccaccaacg atagctccat
     1321 ttatacgtgc accagtcgtt ttatcaaatt tttgagcaat aacagcttga tctgcccatc
     1381 cagtgtttgt cattccatca atgctacttg catatacagc tcccgcaacg actatatcag
     1441 aattgatatc aataactatt ccactataat aatgccttac gttaggtgca gaactggcag
     1501 tccatacaac atttccagca ggatcatatt ttattatata accagctaca tttggtgatg
     1561 caccaacaga aatagtgaga ccattaaaat ttccagactc gaccccacca gttatataaa
     1621 tattcccgga agagtcgact gttatgccat ttgtatttat acttgaagaa cctgaaactc
     1681 caaaaacttt tgtccataat ttatttccat tcagatcata cttacttaaa aaccctgaac
     1741 gagctccgcc tgtagttgga attgtttgac cgtctaaatt cccagttgta gtgccagcta
     1801 tataaaggca attgcagttg gagtcatatt tcatactatt tactccagca tatgtatttc
     1861 cgccggttac tccatttatt acttgccaaa caggatcccc actcccaagt aattgtaatg
     1921 gaacattgaa ggataccttt ccccctgcgt tagtcgccgt aactgtaaaa gaataacttg
     1981 ccgcaggaag tcccgcggga gggttgcaag tgataacacc tgtctttgta tcgaaagtca
     2041 tccaacttgg aggctgcgga gtaatttccc aagaatccac agttcctgta attgttggag
     2101 tgatagtgat tgggttgcca ggaatcagtt tcgattggag tggagagagg gagtaggtca
     2161 ggttgcttgg tgcagaggat gttgaatttg aagcaccaga agaaatagag cctggtttta
     2221 tattgagaac attgattgca aacacaggat ttgaagtatt ggatgcatca aatggcgatt
     2281 tctttgcttt caagcatcca ttgaaaagtg aaacaaataa tataggaaat aaaattagtc
     2341 tcaggctcat aatcctagta acatattaac ttgttccttt ttgcaataat tttttgagta
     2401 aatgatttct tttagaaaaa agacgattaa tagctattaa ccgctttttc ttttattgaa
     2461 caaagcgaac gagtaaatgt gcattgttgg gttttactgg atttgtagtt ccccctatgt
     2521 caccagtaga gtgtcctgtg gcatacatgg tgttattcgt gtcaaaggca attcctgtgc
     2581 ccactgttgt agaaccagat gagcctaaaa gttttaccca tggttgagcc ccatttgttt
     2641 gtaaatttcc attggtatca tatttagcta caaataaatc tatagctccg tttagagtct
     2701 gccctcctaa attaccattg gtctctccaa tgatataata aaaattatct actgattttg
     2761 taattgcgta tgctttggtt gtagtatttc ccgttacacc gagtagacga gtccaaagct
     2821 ttgttccaga agaattaaac ttagcaataa atgcatcata atacgaatcc ccagcagtag
     2881 aagttttagt gataccatcc aagtttctat tagtataacc tgtaacgtaa agattgtctg
     2941 aggagtcacg tgtaattcca aatatatata cttcgcctgc cgttcctcca gttcctgaga
     3001 ctaggctgta acaagcttga tagacaacac ttgaattgta tttaaaaatc gttgcagttc
     3061 tttttaagtc aggattggca catttgtccc ctgcttgtga ataaccacca gtccacaaat
     3121 ttcctgcgga atctgttaca cccgcataca ctcctacgtg tcttcctgtg ctagcggaag
     3181 atgtgccaac taacgtagta gattgccaag ttccagcgct gttatacttc ataacagaat
     3241 ttgctgcatc tgatcctgcc gtattcgcag aaaaatcaag acctccttta gcctgaccaa
     3301 gcaaaaaaac agatccactt gcatctgcat agatagcatt tccaaaagat tcggatgaat
     3361 tttgataccg taagctgctc cattgtcttg ttccatccgg agcaaatttt gaaatcgcag
     3421 cgttatacga attgggagta gtaatcccat caaaagttgt tggaccatag acggaaccac
     3481 caatgtatat attctcagca ctatctacac tcaaaccatt ttgtccaaat ccgcccgaag
     3541 ttgcaataga tagttttgtc cagattcgat ttccatttaa atcatatttt gtgacaaatc
     3601 ctgatgcaac accgggactt ggattggatt ctccatctag agctccgaga gtggcacctg
     3661 ctgaatagag actatttgag tttgaattaa agaaaagact accgtaaccg gctgtcgttg
     3721 cgtatccagt aactccatgg attactgtcc agacattttc accagttcct aaaatttgaa
     3781 gatcgatttt aaattcagta gaaccaaatg cgttcactgc ttgaattgtg aatgttgtaa
     3841 gagcaaaacc tccacttagg taggatgcag agggagttcc tgatatggat cctgttttgg
     3901 aatcgaaggt cagaccagtc ggaagggtag ggttgatatt ccaactatcg actgtgcctg
     3961 ttactgtagg agtaaaattc acagcaactc ctggaatcat tttaaaagaa ctcaaacttc
     4021 cgtagctaac actacttggc ggtgtgattg cgttaggcgt tgttcctcct cctagtattg
     4081 taggttttat gttaaataca ctaactgcga atactggatt cgaggtattg gatgcatcaa
     4141 agggtgattt ctctgctttc aaacaaccta aaagagagat acaaaaaaat atttgcgaga
     4201 ttgctattct tttcatttca acaaatcact ttaattttct ttctatactt ttctgaaacc
     4261 aaatctagta taaactggct ataactagac tttaaatata tgactattct ttaaaaaatg
     4321 aaataaccaa aaaaatcaac tctttttaaa gtctgccctt tattgcacta gaggaaacaa
     4381 gttaacgtta atggaaacga gatcaaaaga taaattgatt tgtaaaaaaa tgaaaagagt
     4441 gtcgcttgaa gattctatct aattgttatt ctcaatagga gacaccaaaa gcgagatttt
     4501 aagttaaaaa gagtgggctt gttttaaacg catccttctc ctctggaacg ttagtttcgg
     4561 tcgtagtgta atttttacct agaggagaga tttgtgagaa attactaaag tattgttttt
     4621 aattattttt ttatagattg cccattatta ttttcttata ctcaattttt aaatctttta
     4681 aggagtagac ttttttggct ggaggcaatg catctaatat ctgtttgccg taattctttg
     4741 tctgcattct aggatctaag atagatacaa ttcctgtatc tgttttagaa cgaatgagtc
     4801 gaccaaatcc ttgtttgagt gtaaggatag cctgaggcaa ttgcatttcc ccaaatggat
     4861 ttccattttt ctttttaatt tcatccattc ttgcctcaag aaccggttcg tttggaggct
     4921 ggaaaggaag ttttgtgata atcactgatt ttaacttatc tccttttata tcaatacctt
     4981 gccaaaaagt agaaactcca aataaaactg aattttctgt atttagatat tctgcttttg
     5041 ctttgtcagc cccaagttct ttttgagaaa agataggata tttacatgta tctactatcg
     5101 catcataaac tgtttgcaat gatttaaaag aagtaaatag cacaaaagcg tttccctctg
     5161 ttaagtctag taacattgga atcagttttg aaatatcatc atggtattca tctggctcag
     5221 ttgctggatc tcttacattt ttaggtaaat aaatcaagca atgcttttcg taatcaaaag
     5281 gggaggctaa taaaatttct cctgcttcaa tttttccgat tttattttta aagaatttaa
     5341 aatctttttt tccactagat aaagttgcag aagtaagaat catattttct aaacgaggaa
     5401 ttaatttttc ttcaatgatt ttttctggat acattggttg catggatatc tttaaaaact
     5461 tttcgttttt acgctcttca ggtggttcta cccaaaccac catttcttta tctttgttat
     5521 ttctgaattg ttctataact tctcctgttt cgtttaactt cttgatagac atttctaaat
     5581 ctagaatcat ttctttgtct tgcaaatcat cggaatcttt gttataggat tttttagccg
     5641 cctctagctt atctgctaat ataaataaga gtccctctaa gactccatca tctaaagcaa
     5701 attttttttg aattctctgt gcgttaaatg acatagggac ttctgaatat agcttgttaa
     5761 aaaaaagaat aatcgattta ttaatattgg atattacttc taagcactta tctctaattg
     5821 tggaatcttg taatttatga atcagtcctt gtcttctttc ggttccgcct acaaattgta
     5881 tgattttatt tatatcttca taactagttt tgagtccgaa ggatcgtcct aaaatatccg
     5941 gaaagctatg agcttcatct ataatcaaat ttttaaattc gggaagtatc ttaaattcgc
     6001 ctgcaatatt tgcagcaagt aaataatgat tcacgattag aatatttgct ttattccatt
     6061 tatctttttc taaaaaataa taagattctg agaaattagg acatcttctt cctagacaat
     6121 tatctgcgtc ccttgtgatt tgattccaaa attcgttcgt tgcaaaccca gtatattctg
     6181 atttgattcc tgtttctgtt tcactttccc attcataaaa aggtttcaga tgatttccca
     6241 tttcgatagg gaaacttcca ttcgagatta cagattgtaa tttccttttg caaacataat
     6301 tatttgcgcc taacgctatt tccgcggtga tttttttccc gagaattttt gctacgattg
     6361 gaatgtcttt tgcgattaat tgattttgta gagcgattgt ctcagtggat ataactactg
     6421 gcacttggtt ttcaagagag tagaccgctg caggaagtag gtaggcaaga ctttttccga
     6481 ctccagttcc agcttctact aaaagatgtt caccagattg aaaggcatcc tctactgcct
     6541 ttgacatttt gatttgttct tctcgaagtt cgaattctga ccatttcctt ccaatttttt
     6601 tggaaaaaat ggattggatg tcgtggtctt ctgttaggct catgatgtca tctcaaggat
     6661 ttccctttgg tttacaatga gaaattttaa caattgaaaa ttctaatcct atagatattg
     6721 gatatttttt taaaatccac tttctaaatg gaatttaaac ttaaatcttt ttcaaaaaac
     6781 attttatttc ttctattatc cgataccaaa aggaaaatcc catttttaaa aatcaaaagg
     6841 tctgtttgat cgaaagagtc ctgattgcca aacttcagta gatgcagtgt cccgaatact
     6901 aatcaaataa attccatgtg aattcctttt atgattaggt atagaaatag aatttatatt
     6961 cttaaacttt tatgtttgaa cgcttttaag tgatttgcaa ttgattctat tattaattta
     7021 gagacttgtt gatttagtga gattgactcg aagactatct ccatcgagtt cctgggtttt
     7081 tactccgcgt ttaatcgttc ttaccttgtc gcctttaatg ttaatccgaa ttggatttct
     7141 taatctactg ttcttcccat cagttagtaa ttgaacaatt atatctgcac cgtcaggatt
     7201 tgttttgtaa tgaataattt gtgctaattc atttgtagaa agtaagacga gtgatccttt
     7261 tgtgagttgt gtttttgcag caagtgagtt ttgtatatca gcaataaatt tttctgcgat
     7321 cctaggagca aaatgcgttc ccgtataagt ttctattact tgaattgatt cttcggggga
     7381 gaaggctggt ttgtatgatc taactgtagt taacgcatcg taaacatctg caagtgctac
     7441 aatcactgaa taatcatcca gtttgtctcc tgagattcca agtggataac ctttaccgtc
     7501 ataacgttcg tggtgctcgt atgccatatt cacaatccta cgatttagat ttttatccaa
     7561 tttcataata cgataggagt gagaagggtg ggatttcatg attcttttct cttcttcaga
     7621 caagacagta gtcttgttga ttaggcttgc gggtattgca attttgccta tgtcatgcaa
     7681 aaaacctcct aatccaatca tttggacttc cgattcggga taacccattt tctgagcaag
     7741 tgctagggag ataattccta cattgatgga gtggcaaaag gtatagtcat catggttttt
     7801 aatatctaga aggttgataa tagcggaagg attggtttta atttgatcgt aaacagcgtt
     7861 gatcgagttc ttgcaggatg taaaatcgat agaatcattt ccatttaata gattgaaggt
     7921 aatgcttcta aaactggaaa tagattcttt gattacctct tttgtgattc ctccatagtg
     7981 aaatccatct agaatttttt cgattttctc ttcttctttt ttgtcttggt catttttttt
     8041 agttttttct tggagggggt aaaactctac ccattctacc ttttcagatt ctaatttttt
     8101 taaaatgtcc tttgtgaata cagttctgtt tgcaaaaaga gtcttgccat cctcgtaaca
     8161 aattttacca tctaggatgg ttcctgcctt gatttggttg attcgtagtt tcataggtat
     8221 ttcaattgta tttactgttt ccgtttctct tttttttaca caaaatcaga tttttcgcct
     8281 agaatcaata ctctatcgaa tcatttctaa aaaacaagga ttttttattt cttatcatta
     8341 tgaatcagtt gactttaaac ctacttcaaa aaaagtgata tttcgggcta ttattagtca
     8401 tttgataagg ctagttttgc cctcttagct cagcggtaga gcacttccat ggtaaggaag
     8461 gggtcgccag ttcaagcctg gcagagggct tattagttct gattcaggcc tgtagtttaa
     8521 tggtagaact aggatctcca aagtccttgg tgggagttcg attctctccg ggcctgccaa
     8581 gagacaatat agtttagagt tattattatg aaaacattaa catttttaca agagtgtaga
     8641 gcagagttac aaaaggttca atggccatct agagaagaag ttatcaactc aacaatggta
     8701 gtattaggca cagtaatcgt tttttctctc tttttgttta tctctgactc attatttgta
     8761 agactcttaa agtggttttg gagtctttca ggttaggtta aaattatggc aattaagtgg
     8821 tatgcattac aggtctattc tggtcatgag aataaagtga agaccaacat tgaaaagatg
     8881 gtcgcacaac attctctaga gaataagatt acccaaatca aaattcctac catggaagta
     8941 gctgaaatgc gcaatggtaa gaagacaatc acaaagaaga aattgatgcc tggatatgta
     9001 ttgatcgaaa tggaaatgga tccagatact caatttatgg ttcaaaacct cccttctgtt
     9061 tctacctttg ttgggaataa agcacctgaa ccacttactt tagaggaaat taaaaacctc
     9121 tttaatgaaa tgggtgatgt taaatcagag gaaactgcaa aaccaagact acttttcaat
     9181 gtgggcgaaa ccctcaagat agtagatgga ccttttgcaa atttcagtgg agtagttgat
     9241 gaaatcttcc cagataaagg aagacttaga gtgaaagtcg aaattttcgg acgctctact
     9301 ccagttgaat tggatttttt acaagtagga aaaagttaaa attattttta taaataaggg
     9361 tttttagaaa tggcagctaa aaaatttgtg aaacaaatta aattacaagt ggaagcaggg
     9421 aaggcaaatc ctgcccctcc agtaggacca gcacttggtc aagctgggtt aaatatcatg
     9481 gaattttgta aacaattcaa tgaaagaaca aagggacaaa tcggaatgaa acttccggtt
     9541 gttatcagtg ttttcagtga taagagtttt acatttgtaa caaaatcacc tccagctgct
     9601 ctactcgtta aaaaagcatt agggattgaa tctggatctg gaacaccgca cgttacgaaa
     9661 gtgggaacaa ttaccagaaa acagttagaa gaaattgcaa aaacaaaaca agctgatcta
     9721 aatgccaatg atttgaatgc tgctgtaaac attatagcag gaacttgcag atctatgggc
     9781 gttactgtag caggttaaaa gggttaaata tgaaacgagg aaaaaaatac aaagcactca
     9841 aagataaagt taacagagaa aaattataca accttgcaga tgcagttggt ctcgcaaaag
     9901 aaacatctta tacaaagttt gatggttcta ttgaaatcgc tacacaagtg aactataaaa
     9961 gcttacaaaa cattcgtggc acagtatctc ttccgcatgg aactggtaaa acagttcgtg
    10021 ttttggtttt ctgcaaagga gacaaacaaa cagaagctaa gaatgctggt gctgagtttg
    10081 taggggatat ggatttaatt gcaaaagttg cagaaggttg gacagatttc gatgcttgcg
    10141 ttgctactcc tgacatgatg aaagaagtag gaaagctagg acctgttctc ggtcgtaaag
    10201 gtcttatgcc aaaaccaaaa gctggaactg ttacaactga cgttgcaaaa gcagtcaatg
    10261 aactcaaaag tggtaaagta gaatatcgtc ctgataaagg tggagtgatt cacttaggag
    10321 ttggaaaggt atctttcgac aaacaaaagc tagtagaaaa tatccaagcg gtagttgcta
    10381 cacttttcaa agatagacct tcagatgcta agggtgatta tcttaaaaat tttgctgtta
    10441 gtgcaactat gggcattggt gtaaaagtag atgctaaaga actagcaaat tcagtgaact
    10501 aaaagagaga ctaaatcatg gcgaatcaaa aaaatattga aacagttgcg gaattaaaaa
    10561 caaaactaga agcaaaacct aattttatat tagctgcata cagcggtttg actgtaaaaa
    10621 acatgacaga cctaagagta agcttattaa aagaaggatc agaaatgaaa gttctaaaga
    10681 acaatttatt tctaagagct ttaaaagaat ctaaaagcca ttctgataag caaatcgatt
    10741 ttggtgctga atataaagga ccaatcgctg ctatcttttc aaaagaaaat ctaccgacag
    10801 tagcaaagat ctgtaaagac tttgctaaga caaatgaaaa cctagtaatg aaaggtggat
    10861 tttttgacgg aaccgtttta gatgtaaaag gtgtagaagg aatcgcagga ctcccatcga
    10921 gagaagaact cttaacacaa atcgcatttg gaatgaacaa tgtaatgtct actcttgcaa
    10981 gagcaatcaa tgcagttgca gaaaagaatg caggtggagc agctgcgcca gcagccgagt
    11041 aaatggttgg aaccgtttat ataaacggtt ccacagaatt aaacaaatag taataatttt
    11101 ttaaaaggac aataaggaga aaacatgtca acagaaagtt tattagagca gataggaaat
    11161 cttactttag ttcaggcagc tgaactagta aaagcgatgg aagctaagtt tggtatctct
    11221 gctgcagccc cagtaatggt tgcaggcggg ggcggtggag cagcagcggc agcagctgaa
    11281 gaaccaactg aagttagtgt tattctaaaa gcgcatggtg ataaaaaaat cgatgtaatc
    11341 aaagcagttc gtgagattac aggtcttggt ctaaaagaag ctaaagacct agttgaagct
    11401 ggtggcaaac cagtgaaaga aggcatttca ccagatgaag caaatactat caaaaagaaa
    11461 ttagaagaag ttggcgcaca agtagaaatc aagaaataat ttctattttg cattttactt
    11521 ttgttaaact atataatttt ctacctcacc tgcaaaagag ggggtagatt tttatattgt
    11581 ttttgtattt tttaaagcag tttttaaaaa aatttaaaat ctgttttttt atcaaatttc
    11641 ttaaagccga aaggctcaca ctccaggagt ttaaatttta atgattactc aaactgaacg
    11701 caaacgggta aactttggca agattacgaa tctagatttt cttcctaacc taatacagat
    11761 tcaaaaaaaa tccttcgact ggtttctaca agcagacgtt aaagatccaa ctaaacgaaa
    11821 aaaccaaggc ttagaagcag ttttccaaga aaccttcccg atcgaaagtc cgaatgggga
    11881 tgtaatcatg gaatacagcc attatattct tggagaaaaa agaagagatc agcaagaatg
    11941 taaagataca gattctagct atgcaatccc attaaaagca gtcattcgtt tgattatcaa
    12001 agaaactggt gagattcggg agcaagttgt ttacatgggc gacttaccga ttatgactga
    12061 ccaaggaact tttatcatca atggggctga gcgggtggta gtaagtcagt tgcataggtc
    12121 tccaggtatt ttctttgcct atgatcaaat taagtctgct tattctgcaa gagtgattcc
    12181 ttatcgtgga tcttggttgg aatttgaaat tgataataaa ggtatactgg ttgcgaaaat
    12241 tgatcgtaag aaaaaatttc cagcaacact tctgttaaag gcactaaaag ttggcttaaa
    12301 cgaagatgta ttgaaattat tttatacctc ctctaaagtt aaaatctatg gagcctctcc
    12361 aaaagaatta aagaaagtaa tcggaagaag aacgatatcc gatatcatta actttgaaac
    12421 cggtgaagta atgctcgagg ctggagctaa gatcaatgag gataatattg atattctcaa
    12481 agaaatgaaa gtcaaagaag tgaatgtaat tgattatcca aatggaaaag acaattcaat
    12541 cattattaac tgtcttgaga aagacggtgt aaacgactac gaagaagcaa ttaaaaagct
    12601 acatatgatt atgcgtccag gtgagccttc gacgattgaa aatgcagagg cagaactaaa
    12661 acgtttattc ttttctccaa aaacctttga tttaggaaaa gtgggtcgtt acaaaatcaa
    12721 tagcaaattt gaattccata aaccgaaaga attcacaaat tgcgataaca gaactttaag
    12781 acctgaagat attatagaga cagtgcgtta tttagtaatg ctcatgtcag aagttgaaaa
    12841 ttattaccat gatgatatag atcaccttgg gaatagacgt gttcgttctg ttggagaact
    12901 tttatctaac caattaaaac ttggtttttc tcgcgtagaa agagttatca aagaaagaat
    12961 gactgttcaa gaaattgaac aacaaacacc acaattatta atttccatta aacctgtaac
    13021 ggcagttgtg aatgaattct ttggatcttc tcagctttct caatttatgg atcaaaccaa
    13081 tccattagca gagttaacac ataagagaag attaaatgcc ctcggaccag gtggtctttc
    13141 aagagataga gctggtttcg aagtgcgtga cgttcattat agtcactatg gaagaatgtg
    13201 tccaattgaa accccagaag gtccaaacat tggtttgatt ctttctatgt cttcctttgc
    13261 gagagtaaat gattacgggt ttttagaaac tccttataga actgtaaaaa gtggaaaggt
    13321 tcaaaaacaa atagaatttt taacagctga taaagaagaa tatagataca ttgcacaagc
    13381 gtcagctgct attgacgaaa agggagattt taaaaataaa ttaatctcta cacgtcatag
    13441 atcagatttc ccttttagaa atccaaatga aattcaatac atggatttgg ctccaatgca
    13501 agtagtttct gtttctactg cactcattcc attcctagaa catgatgatg cgaaccgcgc
    13561 actcatgggt tctaacatgc aacgtcaagc tgttcctcta atggtagagc aggctcctta
    13621 tgttggaact ggtatggaag gaagagctgc ttatgattct cgtatttgta ttattgcaaa
    13681 gaaatctggt atcgtagtaa aagtagattc aaaagcaatc agtgtaagag aggagggggc
    13741 aaaagaatta acagtttatc ctctcatcaa atacaaaaag acaaaccagg gaacttgttt
    13801 caaccaaaag ccagttgtat cagtaattat ggctgagaaa gatggaaagg ttgtaaaaat
    13861 ttccaaagat aaaatggaag ttcagtatgg ggaagaaaat gcaacatact ctcttctcta
    13921 tggaacaaaa gaatttgcac cactggtgag agaaggtgct tctgtttcca aaggtgaaac
    13981 gttagctgga caagtggtaa ctggtgaaaa gattgatgac caaggaaata ttttagtaaa
    14041 aggaactatc ttagcagatg gtcctgcgat tgacaatgga tacttggctc ttggtaaaaa
    14101 cgttttagtt gggtttatgc catgggaagg atacaacttc gaggatgcta ttatcatcag
    14161 cgaaaagatt gtaaaagatg atatctttac ttctatacac atcgaagaat ttgaaatcca
    14221 agcaagagaa acaaaactag gtcaagagca aatcacacga gatattccaa atctctctga
    14281 caaagcattt agagatttag atgaaacagg cgttatccgc attggtgcgg aagtaaaagc
    14341 tggggatatc ttagttggta tggttactcc aaagggagaa actgacttaa ctcctgaata
    14401 taaattgctc cattctatct ttggagaaaa agcaaaagaa gtaagagatt cttctttgag
    14461 acttccaaat ggaaacgagg gaactgtaat tgatattaaa cgttattaca gagagcgtgg
    14521 agatgattta gctgcaggtg tagaagaatt agtaaaagta tatgtagcaa gaaagagaaa
    14581 actactcgtt ggggataaaa tggcaggtcg tcatggtaac aaaggggtag tcgctcgcgt
    14641 cgtggcagaa gaagatatgc catatatgaa agatggaaca ccactcgata tcgttttaaa
    14701 cccactcggt gttccttctc gtatgaactt gggtcagatt tttgaaactc aattaggttt
    14761 tattgcagat aagctaggta ttaattttga aactcctgtt tttgatggag caaatgaaac
    14821 tgatatcgaa gaatatgcaa aagaagcttc tgctcctaat aactctaaat tccaactctt
    14881 tgatggaaga acaggggagc cattcttaaa cgaagtcttc tgtggacata tttatatgct
    14941 gaaattggct cacttggtcg atgacaagat acatgcaagg tctacaggtc cttactctct
    15001 tgttacacaa caaccactcg gagggaaagc tcagtttggg ggacaacgtc ttggggaaat
    15061 ggaagtatgg gcactcgaag catatggagc atctcataca ttgcaagaac tactgactat
    15121 caaatctgat gacatgctcg gaagagctcg tatctatgaa gcgattgtaa aaggtatcaa
    15181 ctcgattcgt cctggtatac ctgagtcgtt taacgttctt gtgcaagaac taagaggttt
    15241 agcacttgat attattatta cggatacaga aggttcaact gtagacattg ccgactacga
    15301 agatgaatat tctagaaaca aaaagaaaat taaaattgaa tctattgaga acatatagag
    15361 gcgactaatg aaaaattata ctagttttga ctcgatcaca attaaaatag catctcctga
    15421 gagaatcaaa gaatggtctt acggggaagt aaaaaaaccg gagactatta attatagaac
    15481 tctcaaacca gaaagagaag gtttattctg cgagaaaatc tttggaacaa caaaagattg
    15541 ggaatgctat tgtggtaagt tcaaatcaat ccgttacaaa ggtgtgattt gtgataaatg
    15601 tggtgtggaa gtaacgcact ctaaagttcg aagagagaga atggggcata tagaacttgc
    15661 agctcctgtt tctcatatct ggtattatag atccgttcca tctagaatgg gattacttct
    15721 ggatatgaca atgaatgctc tcaaaacaat tctttatttt gagaaatatg taatcattga
    15781 tccagctgat tcaggtcgcc ttaaaggtga acttttagat gaagatgaat accattctta
    15841 tctagatgaa tacggtgata aatttatcgc tggtattggg ggagatgcga ttaaagaatt
    15901 actcgctcgc attgatgttg atgcggaagc gcgcgttatc cgccaaaaaa tccaagagaa
    15961 aaaagttacc gacaaacgtt taatcaaacg tctcgaagtt ctagaagctt tccgtgattc
    16021 aggaaatcgt cctgaatgga tggttctaga taacgttcct gttatcccgc cagaactcag
    16081 accaatggtt caattagatg gaggtagatt tgctacttct gatttaaatg atttatacag
    16141 aagagtgatc aaccgaaaca atcgtttaaa aagacttcta acactcaaag ctccagaaat
    16201 cattgttcga aatgaaaaga gaatgttaca agaagcagtt gatgcgttat ttgataactc
    16261 tcgtcgtaaa agaactgtaa aaggaaaagg aaataggcca ctcaaatcta tttctgatat
    16321 gttaaaagga aagcaaggtc gtttccgtca aaatttactt ggtaaacggg tagattattc
    16381 gggtcgttct gttatcgttg taggacctga gttgaaattc caccaaatgg gtcttccaaa
    16441 gaaaatggct ctcgaattgt tcaagccatt tatcatgaaa agacttgtgg atttagaact
    16501 tgctccaaat atcaagtctg ctaagaaaaa agtagaagca gaagataaag atgtatttga
    16561 agcattggaa accgttgtaa gagaacatcc tgttttatta aaccgtgctc caaccttaca
    16621 tagacttggg attcaagcct tccttcctgt tcttgttgaa ggaaaagcaa ttaaacttca
    16681 tccacttgta tgtcatgcgt ttaacgctga cttcgacgga gaccaaatgg caattcacgt
    16741 tccattgtct ccaaaagcac aattggaagt ttggatgctt atgctttcgc ctcacaatat
    16801 tttgaatcca gcgaatggac atccaatttg cggaccaact caagatatcg tattaggtat
    16861 atattacctc acaagtgaag tgccaaatgc aaaaggtcat ggtaagtttt ttacaaacct
    16921 agatgaaatc caatatgcgt tagatggcgg atggttagat ctaagagcaa aaatcagcgt
    16981 tctctataac gagaaaatca ttgagacaac cccaggtaga atgatcttta ataccgcaat
    17041 gccaaaaggt tacaactttg taaacagagt tctcggtgat aaagaaacaa acaaaattat
    17101 ttcggaagta tacgatacat ttggacctgc agcaactgta gtgatgttag atgacattaa
    17161 gaaattaggt tatcgatatg ctactatttt tgctccaact atttcaattg aagatattag
    17221 agtttctcca caaaagcaaa ttctagtatc agaagcaaac aaagaagtag aaaaagcgga
    17281 tatggaatac cgaaaaggta ttatcacaaa tgaagagcgt aagaaaaaag taatcgagat
    17341 ttggacaaaa gcgaatgatc gtattacaga tagtatgttc aaagagctcg aaaaagatag
    17401 aggtggattt aaccctgtat acgtcatggc tgcgtcaggt gctagaggtt ctaaacaaca
    17461 gattcgtcag ttggctggaa tgcgtggact tatggctaaa ccatctggag aaattatcga
    17521 actcgcgatt cgttctaact tccgcgaagg tcttggtata ttagagttct ttatctctac
    17581 tcacggtgct cgtaagggtc tctctgatac tgctcttaaa acggcggatg ctggttattt
    17641 aactagaaga ttagtagata tttcgcaaga tgttattatc aatgaagtag attgtggaac
    17701 agatcaatct gtagagttga gtattgtaaa agaaggggaa aatgtaatcg tttctcttgc
    17761 agatcgagta ttcggacgtt atacggcgga agaagtgatc gatccggtta cagataaagt
    17821 tgtatatcca aagaatactt tgattgttcg atctgttgga caaaaaattg aaaatctagg
    17881 ttatacaaaa attaaggtta gatctccgct cacttgtgat gcaaaacatg gtatttgcgt
    17941 aaaatgctat ggtatggata tggcaagact tgttccggtt gaaattggag aggctgtagg
    18001 aacgatcgca gcacaatcaa ttggacaacc tggaactcag ttaacgatgc ggactttcca
    18061 catcgggggt gctgcttctg caaaagtgaa agaaaaggaa cacaaagtat ctttccaatc
    18121 tgttgtgaaa ttaatcaatg gtagactgat tcaaaatcaa aaggggcaaa cagtatttag
    18181 ccgccgtggt tcaatcgtag ttcaacattt gatccaagat ttacaattat cagatcttca
    18241 aaacattcgt gtgaataatg gacagaatgt attaaaaggg gaaatgattt ctactaactc
    18301 taaaggggaa atgatccatg cagaaatgcc tggtagaatt gaaatctctg gagatagatt
    18361 tagaatcata ggagaggaca ttgttattcc agttaaaatt ggaactattc ttctagtaaa
    18421 agaaaatgat attgtaaaag caaatagccc gattgcagag tttgatcctt tcaacgattt
    18481 agtgatttcg gaagtagaag gaacagttac ttgggttgac atggaagttg gtaaaaacat
    18541 cagaagagat gaagatgtga aaacttctaa cgttatttat aaagtaattg agcaaaagag
    18601 agaaaagcta aatcctagaa tggttgtttc aaatggtgat acaacagaag aatatgcagt
    18661 tccagtggat gcgattatta caattgtaac tgacgataaa gtgaaaatag gggatatctt
    18721 atttaagatt ccgtctatcg ctgtgaaaac aagggatatt acaggtggtt tgccacgcgt
    18781 agatgaattg ttcgaagctc gccgtccaaa agatgcagca actttagcgg aaactagtgg
    18841 acgtatcgaa gatagaggag aaattgtaaa agaaaaacga gtattctata tcatccctga
    18901 taatccagat atggagagaa ttaaagttac gattccactc gcaaagcaat tacgtgttca
    18961 agatggggat tatgtgaagc aaggagacca gttagatgat ggaaactttg acccacatga
    19021 tattctaaga gttaggggaa ctaacgcact tcatgcttat ttagtgaaag aggtgcaaga
    19081 ggtgtataga ctccaagggg tgcatatcaa tgataagcat attgaagtcg ttgtgcgcca
    19141 gatgctacgt aaagtgatga ttactgatag tggagatact tcctttgtat ctcaacaaca
    19201 agtagaccgt tttacattcc gagaagaaaa taaacgagtg gatgcagaag ggggaagccc
    19261 ttctcaagca acaccaattc tacttggact tacaaaagca tctttgaata ccgaatcctt
    19321 tttctcagca gcatcttttc aagaaactac gaaagtgtta acagatgcag ctatcaaagg
    19381 aaagacagat caattgatcg gtctcaaaga aaatgtgatt attggtcata tgattcctgc
    19441 aggaaccggt atgagaaaat accaagaaat cgatgttttc aaggatttct acggtgattt
    19501 agataaaatg accgcagaag aggaagaaga aaagtttcca caaatgcaaa cgaattcatt
    19561 agcagtgcaa atcactgata aaaatgattt agaatcagat gtggatgatg aagaggatga
    19621 tgagtgaaaa caaaatttgt tttcacttta caaaaaggaa taattaaaaa atctgacagt
    19681 agagagaact gttattgtaa atttagagga atgaatgcca acaattaatc aattaattcg
    19741 acacggaaga aataaacaaa aaaagaaaac taagtcccca gctttaaaaa gttgtccaca
    19801 gagacgtgga gtttgcacaa gggtaatgac atttacaccg aagaaaccta actcagcttt
    19861 aagaaaagta gcaagggttc gactcacaac tggtattgaa gtaactgctt atatcccagg
    19921 ggaaggtcac aatttacaag aacacaacgt ggttctcatt agaggtggta gggtaaaaga
    19981 tttaccaggg gttcgttatc acattatcag aggaacgatt gatacgctag gtgttgataa
    20041 gagaagaaat ggtcgttcta aatacggtgc aaagagacct aaggcgtagg agagagcaat
    20101 gtctagaaga agaacaaaag cagaagcaaa aaatatcgta ccagatatta aatacaatga
    20161 taaagtcata ggcaagttta ttaactgcct tatgtttgat ggaaagaaga gcgtagcaga
    20221 aagaattttt tatgatgcta tggacaatat ccaatctaaa ctcagttcag atccttataa
    20281 agtgtttcat gaagcactaa acaatgtaaa accagaagta gaagtaaaat ctagacgtgt
    20341 tggtggggtt acttatcaag ttcctatcga agttcgtcca gaaagaagac ttgcactcgg
    20401 aatcaaatgg atcattcgtt attctagaga gagaaacgaa aaatctatga gctctaaatt
    20461 agcagcagag attttggatg cccacaaagg aactggttct tctatcaaga agaaagaaga
    20521 tatcagaaaa atggcagaag caaacaaggc attcagtcac tatcgatggt agtctgattt
    20581 taattagtag gaaatgtaaa tttccgatac tgcaaagagc cggtaaaaaa ccggcttttt
    20641 ttttgtcttg agtaggatag aatcaatttc aggataaaat gaaaaagctt gacagtggtt
    20701 tgtaagctat caggagtaat gtatggcctc ccatatatta gaaagagaat tccttcgaga
    20761 aaattttatt cctattagtg ttgaggcata tcattaccta gctgaaaaaa atttaatttc
    20821 tgagaaagcg gaattaattg agggagtaat cgtaaagaaa ctgcctaagt caccaattca
    20881 tgcagcgtta gttcgcaaac ttatccattt tttccaaaaa tcaatctcta atcaatttac
    20941 catttcgagt gaaaatccac tgatttttag ccattctgtt ccagaaccgg atttagcaat
    21001 tgtggaattc agagaagatt tttatgcgaa agaacatcct agctttgcgc acctagtaat
    21061 cgaaatagca ctttcaacca atgcatttga tagagataaa gcagatatct atgcatctgc
    21121 aaaaattcca gaatattgga ttttcaatct gcaaaataaa actctagaag tttttcaaaa
    21181 cccagatcaa gacaaatacc aaacgacctc tgttctttcc gcttccgatt ctatacatcc
    21241 actctttcaa aatgagattc ttttagattt aaaagaattc ttatcctttt aataaaaaga
    21301 tttaaagatt actgatagag acagataaaa atacgatagt ctctattaag ttaagatatt
    21361 ttttgatgat agaactatct taataagcaa tgctgctata ttttatgtat tttgattaaa
    21421 gggaaagaaa tgaaatgaaa aaaaatttta tgtataactt tggtagtgct tagtttaaat
    21481 cttttcttgg ttttttgtca ctatgaaagt aatgattttt acttatataa aaaacttgta
    21541 tttgtaaatt aagcgaaaaa caatacaaaa cgcctttgaa aaatactagt agacattcat
    21601 tgcaaacaag aatggatcct atgattgtgc atgataaaac ctttggagca ggaatacaag
    21661 tttacagcaa agataaaaga atggaagcac tgtttagtcc tgaagatgaa attataaaaa
    21721 ttaattcttg tgaaataaaa attgcagata agatatctta tgaggaatcc aataatgcgg
    21781 taattggtga tgattttttg gtagatgagt tactgcattt tcaatctatg ttcagtgatt
    21841 tagaacctga cgaaaaaata tttttaacaa cgattcgaaa taaagataca atagttattg
    21901 aattttctga gaagctgttg caagctacag ctaactcatt aaagaaataa aaatagtaat
    21961 agttcagcct cccttggaaa tactaagtta atcatcctta caaccttcgg cttagctttt
    22021 attattctat tttaaattaa ttaaaattaa ataaaggcta acactcttta tccgtaatga
    22081 atctaaaagg aaaaagattg ctggatattc ttcaaattta ttaaactata agtttgaaag
    22141 cattgcttct gaggaagaaa agtaggattc aattttaaag tgcaaagagg tcaatatctc
    22201 aactttctaa gggttttgct ttactagtga gaaacttcac ttaattttta gaaagaatag
    22261 aatcgtatca ttctggaaag aagcagaaag aaagcaaaaa agcctctttc gaaaaaattt
    22321 cttgactaat tgtttttaaa atatcaactc agtgaataaa taaaaaatcc attgaaggca
    22381 ataggaaaaa tatggctaaa aatcaaagag tagtgatcac aggtatcgga acagtattac
    22441 caaatgcttc tacagtagac agtttttggg aaaatttaag agatggaaag tctcaaattg
    22501 gatatttaac acgctttccc accgataact tttctattaa agttggtgct gaactaaatg
    22561 atttcgatta caaaaagtat ttgcctgact tagatcctaa actcgcgaaa aattacaatc
    22621 atgagacact cgttctaatg gcagcaatgg atttagcaca aaaaaatgca aagctcgcaa
    22681 aagatagctt agatcctaca agagttggtt ttattgattc ctcctctcgc tcttctcttg
    22741 cttggtggga acatgcttgg aagagataca cggaattaaa agatgagtct gtatttgatc
    22801 gttatgcggt attgacttct atggcatcta atccaacgag tttaactgcc attaatagta
    22861 atattcaagg ttttgttaca actatttcct ctgcttgtgt aggaggacat catgcaattg
    22921 cactttgttt tcaagccatt cgtaaaggac gcgctgatgt tatgtatgca ggaggacatg
    22981 aatttccttt gatcaaacca ctcatgcaaa tgtattctga tccacaaagt cgtgtaatgt
    23041 ctctagaaaa agaggatcca aagaatgcaa ttcgccctta tgatatcaaa cgggatggat
    23101 ttgctctagg agaaggagcg gcggtattat gtttggaaag ctatgatcat gccatggaaa
    23161 gaggtgcaaa aatttatgct gaaatcttag gaactcttag ctataacgaa gcagatcatg
    23221 ctatgagaat ggatattaca ggaaaaaaag catgttcagg cataaaaaaa ctcattcaga
    23281 ttagtaaacg ctctttagag gatgtagatt atttctgtgg tcatggaact gctacctata
    23341 caaatgattt agcagaaggt agatctatga agcttttgta tgatggtgtt ccaaagaaca
    23401 agtgggcgcc tcttggttca attaaaccaa tctatgggca cacatttggt gcagctggta
    23461 ttttaaatgt agcagcaacc gctatgatga ttgagcatca gacactttgc ccaaccatca
    23521 atttaaaaaa tgtagatccc gaatgcgatc aagatcatat tagcgaagga cctagaaaaa
    23581 caaaactaga ctttgcaatt tctcttgctt ttgctattgg aagtcaatct tcctttgtgt
    23641 cgcttggtgt gccagattaa ttttaggaaa acaatttgct tttaatttga gtcattgacc
    23701 tttctttgta tgagttgagt gaggcaaatg gaatacacgc caatttagga tactgataaa
    23761 tattaggttt ttctaataga taaaaaagct gaatgaaatt tactaaaaaa gattaaatgc
    23821 aaagcatgtt caaagaactt tattttctag ttaggaatga ataggaggaa tcatgaaact
    23881 taaatcaaat ggaattcaaa tcacaggcta tggagactat tatccagaga agattttaac
    23941 tgatgaggag attagaggta gattaaaata tccagatatg cacccggcag agaaggctgt
    24001 gatcggggat attggtgtta cccaaagaca tcgatccagt gaaaaagaaa cttcggtcta
    24061 tatggcatcc aaagcttgtg aaatggctat gaaaaatgca ggtgtttctc ctgaatcgat
    24121 tgatctatat ttgttctgca attggacgga aagatactat ctacctgatt tggctcccca
    24181 ggcttccctt ttaaatggaa ctaagaatgc acttgctttt gatttaggga ctgcttgcac
    24241 tggatttgtg catggagtgc aaaccgcatc ttgttatctc caaacgggaa aatggaagaa
    24301 ggctctcgtt gtaggttcag aacgtttttc agaacgaaca aagaaggggg gatatggcga
    24361 atttactgct ggggatgcag cggctgctgt agttatggaa tatactggaa atacagagac
    24421 aggattagta gatacatgtt tgtatgatgt cggaactctt gtggatgtga ttacttgcac
    24481 cgctccagct ggactcattc gaagttatcc agacttagtc acaaatgcag cagactattc
    24541 tttaaaagcc attgatttac tcatggagag aaatgggctc aaagaaagtg atataacatg
    24601 ggttgttcct catcctggaa cagacattgt tgtgaaagat attttaaaaa gaactaaaat
    24661 cccaaaggaa aagtatttaa tgaactatcc taaaatggga aatgtttccg cagcatcgat
    24721 tccaactgtt cttgcagaga attacaataa aggtatcatc aagaaaggtg atgtggtttt
    24781 aactcctgct gttggcggtg gcttttactg gggaggaatt ctctacatcg cataattgca
    24841 tttggtctga ttttgcttct cgaaaggaag cgaaatcagc ttatctaata acttctctct
    24901 tctcattctt tttggccaag gatcctgtga ttcttttttt gacattagga ttaaatccca
    24961 tttgaatatt gggttcacct taccctgatt tttattaagt agtgaaaata gaagttgagt
    25021 tcgtaaatac gttgcaattg ttaggaatcg ttttcggggt aaaacgttta gcgtctttgc
    25081 tgttttgaat ttggaatacc caaacaaaaa aatccccaag ttgtcagcag gcactcttct
    25141 aaaaaaaaca ctatatttat tacacggctt actaatttca tttttcctaa ggaaaatttt
    25201 taccttaaaa aaataaattg catgacaaaa ttaaataaaa gcaaacagtt ctgtaatcta
    25261 cttacagaac cgctgttgcg aggtcttatt tttttatgca gttaattgaa ggctatgaaa
    25321 tcaaggaaag gatcaataca aattcagata tagaagttta tagagccgtt gacaaagcag
    25381 ataacaagcc cataatttta aaaagtattc caattcataa tgaatttcat gcatctatca
    25441 taaatttaaa aaacgaattt gatgtaatga aatacctgtc atctgacttg atgttaaagg
    25501 tctatgcatt tcaaagatat tcaaatggtt ttttcctagc ctatgaagat acaggtggaa
    25561 tttctctgaa agagtataca caggggaaaa aaattcctgt tgataagttc tatgatattg
    25621 caattcgttt ggccgaaatt ctatctgaaa ttcatagaaa aaaagtaatt cataaagaca
    25681 taaagcctga aaatatcatc atcaatccta ataatatgga tgttaagctc attgactttg
    25741 gaatttcaac tcgcctgtct aaagaagaaa ccaaatggac agcagctaac gtcttagaag
    25801 gtagcattca atatatttcc cctgaacaaa ccggtcgtat gaatcgatct gttgactatc
    25861 gtagtgactt ctattcttta ggtgttacat tctatcaact tttaactggt aaactcccat
    25921 ttgaaaatac ggatcttcta gaacttgttc atgctcattt agcaaaaact cccctaacag
    25981 cacatgaact cgattcttct attcctgtct ctctgtcgaa tatcgtagat aaactgatgg
    26041 caaagactgc cgagaatcgt tatcagacag ctcttggatt aaaatatgat ttagaattag
    26101 caaaagaagc taaacctgac caaagtattg taattggctc taaagattta tcagatgaat
    26161 ttaaaattcc acagaagtta tacggtagat cggatgatat tttaaaacta attgaaacgt
    26221 ttgcagagat tcatgaaaat ggagcttcta aactcatgct catttctgga tattctggtg
    26281 taggtaaatc ttcattagtc aaagaaatta ataaaccgat tactgcttcc aggggatatt
    26341 ttttaactgg taaatatgat caatacaatc gtaatttacc attcagcgct atcattcagg
    26401 tttttactaa cttaattcga ttgtttttga cggaaagtcc tgaaagtatt gatacatgga
    26461 aaagtaagat tttaac
//
LOCUS       DAOQOS010000077        25749 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_890_length_25749_cov_5.992878, whole genome shotgun sequence.
ACCESSION   DAOQOS010000077 DAOQOS010000000
VERSION     DAOQOS010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 25749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25749
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_890_length_25749_cov_5.992878"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..598
                     /locus_tag="PLF66_16155"
     CDS             <1..598
                     /locus_tag="PLF66_16155"
                     /inference="COORDINATES: protein motif:HMM:NF024122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HMZ65626.1"
                     /translation="SNVSIEYVQLRVKFFEGDTVESLRDLGVNCYKAHYNGKVFSPMK
                     SLIQALKQNGFEVWIITASPEAMYQKILSEELQIPITNIVGVKSIVREGKITGEMILP
                     IPQDHGKKEAIETFVQERPLLVGGNSRGDKEMIEYSSNLKLIINPDEYVAEDQTESIA
                     EYARKNNWVIAKVRDVPANDFSYISSKDFNIKKNKTNE"
     gene            635..1957
                     /locus_tag="PLF66_16160"
     CDS             635..1957
                     /locus_tag="PLF66_16160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020776094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HMZ65627.1"
                     /translation="MILTEFFKPAPPKERLPEKEIEYKYPKLRWQVLESTFLGYAAYY
                     FVRNNLSVVSKEMGEALQYDKSQIGDILAVTAITYGIGKFVMGALSDRSDSRKFMAVG
                     LLITAGINFIFGTVENYFFHLFLWGLNGFVQGMGWPPCGRSIGHWFSLKERGSVFAIW
                     NVAHNVGGGMVGVVAAFSASHWGWRSAFYVPGILAVISSVYIFIRLRDTPQSEGLPPI
                     EEYKKDNPSKQLTEDSERELETKELLTKYIFNNKFLWLFAIANFFVYVVRYSMLDWGP
                     TYLKEMKGASLTSGGMSVLILEFAGIGSTILMGWVSDRLGGRRGMVSLLCMIPIFFAF
                     AAIYYNPPGNLWIDFTMLGIIGFFVYPPVMLLGVASLDVTSKKAVGTAAGFVGLFGYI
                     GRTAQAKGLGWLANHSEYGWNYVLYSILICTILSILFLGFTWNIKPKR"
     gene            2133..2342
                     /locus_tag="PLF66_16165"
     CDS             2133..2342
                     /locus_tag="PLF66_16165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65628.1"
                     /translation="MIFKKLENTNELIKSITCGKARLNESEKVMGGKPFVPSLSHSSS
                     LLSFFNDNRSKRMVHQNSIIAFVKQ"
     gene            2396..3550
                     /locus_tag="PLF66_16170"
     CDS             2396..3550
                     /locus_tag="PLF66_16170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65629.1"
                     /translation="MATVTEELIYEYWDCDKCGQKEIRGDIRTCPNCGNARNENIKFY
                     RIEGKEEKVTDKAQADKFKAGADWLCSFCETLNSVTDKQCLSCGASQEASKKNYFEVQ
                     KEKEKKKEKNKPNQKPQEQPTKKPFNWKKFSVWALTLLGGCIASIYFLSRTHNVTFQI
                     VDVSWERKVPVLRYTNVQKSDWDDQLSGDDIVKISSAQEVRSYEDRQVGTKTERYTET
                     EQYQSGSRRECRTSYESTGSGASKKVTSCDSVPTYSSRTVTKTREVPVYKKFPIYGTK
                     VTYTANEYKFLREDSLSGKDNEPKWPDVKLGIGIKNKEDLKGEPTATYIVEFKKVNPE
                     DQAKDFQKIMTTEDKFRNVYILNQSVIKQVNIFDEIVLEDGEERIEEKKE"
     gene            3547..4386
                     /locus_tag="PLF66_16175"
     CDS             3547..4386
                     /locus_tag="PLF66_16175"
                     /inference="COORDINATES: protein motif:HMM:NF015100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HMZ65630.1"
                     /translation="MIANLEFKKTNFKDKIISVLQSDVRIVQILLMGTLLSIGVYFRD
                     FSIHPMQIFLTFCSGLATHFLWNRILKIKVAILSTVITCFGLSLLLRSDLFWIHPAIV
                     FLAISSKFIIRIRGKHLFNPAMFGVILGISLFPRTWVSPGQWGFELTIGIWLLVFGLI
                     VSGRAGISDISYAFLLFHFLFLAYRIFRFGYQWDVLLHQFQNGALILFAFFMITDPKT
                     IPDHKIGRILHAFLVAMFSYIWAFVFFKTNPYIWALFISSPVVIFWDYLLKAEKYKWK
                     LEN"
     gene            complement(4785..5279)
                     /locus_tag="PLF66_16180"
     CDS             complement(4785..5279)
                     /locus_tag="PLF66_16180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65631.1"
                     /translation="MTIIPRKFDSEIKITETGRWLFRGQEITQENVLSFFKKNICEDD
                     KGIYITNSYADKVEHGYLEANCIFLKIENAIETSEGIFLQAEDESITPLKDFDFYSDK
                     EEKLFCMRKKDQFIKFKLNRKSHDFISNLLEEENGRYFLKTKEYEIPVTEFSGKIEVR
                     VPAF"
     gene            complement(5251..5691)
                     /locus_tag="PLF66_16185"
     CDS             complement(5251..5691)
                     /locus_tag="PLF66_16185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65632.1"
                     /translation="MRISFSILLILLVFNCASSVDFQPDSDFTEENPIYSKQKPEEVE
                     IVAERPVRDFKMVGTVIFRNFVDPIDMKSELYTLQMEMFRRKIDGVWIYKKTLETIPP
                     LIINTQNGEGMTVAYTEANREMGKLVGYPYRYRKINDNNPKKVR"
     gene            5813..6523
                     /locus_tag="PLF66_16190"
     CDS             5813..6523
                     /locus_tag="PLF66_16190"
                     /inference="COORDINATES: protein motif:HMM:NF025115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4163 domain-containing protein"
                     /protein_id="HMZ65633.1"
                     /translation="MRNYAKKVQLFICFLISFNLCSQSKEPLSIDGKVLDKKEKNCSY
                     KIKYPILKLGKDSKYDVSVVRNIGKVFIDEFTNIEKYENEFECNKKDKNASPFTLNGD
                     FTVKLKTDSILSIHSSFSSYVEGNPYPNNVFKTYNFELNSGKEIAFEDLFKKDSKYLI
                     PLHKFMAEAMLKEKVISEKDEFIALKKEKYDFYLTQNAIVFINLFDIHAMQSVEVMIP
                     YDKMKKYLNTDMVLKFVK"
     gene            6520..7269
                     /locus_tag="PLF66_16195"
     CDS             6520..7269
                     /locus_tag="PLF66_16195"
                     /inference="COORDINATES: protein motif:HMM:NF018400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1186 domain-containing protein"
                     /protein_id="HMZ65634.1"
                     /translation="MNKDQVLEAFQKSGRQLSKDVLFEIRDNSKKYKKELIKILSKEA
                     KKPQDPISKFEQNDWIYSMYLLAEFREKEALSPILKLFSRKGNFLNPLTGDIVLTNLG
                     QIIASVANGENDELKKLVLDTSLNEYCRAAFLEALVVMTELNEMDREDLYHYFKDLFK
                     NLEKEPIFPWSVLAQCSVLLFNKSFLKEIKQAYKNNLIDEIYIDLEEINSYISDPELS
                     QLGLAKKKYGIIKDAITAMDWWANYKEEKVV"
     gene            7266..8864
                     /locus_tag="PLF66_16200"
     CDS             7266..8864
                     /locus_tag="PLF66_16200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HMZ65635.1"
                     /translation="MKLRESNIAKLQKENFDVIILGGGINGAVSAAALAAKGAKVALI
                     DKGDFAGFTSQNSSNLAWGGIKYMETFEFGLVRKLCMSRNKLMRNYPSTVEEIRFFTT
                     ITKGFRHSVLKLYAGTLFYWLMGNFFTKPPRYLSKGRINQEEDIINLENAVGGFEYSD
                     AYLHDNDARFVFLFIRSALNYGCIAANYVESTGASKVGDFWNVKAKDNISGKTFTLRG
                     KVLINSCGPFVDEHNQLTNQKTNHRHVFSKGIHLIVDRLTETKHILTFFADDGRLFFV
                     IPMGNRTCIGTTDTQVTSPYAEVTKEDREFVLANINKRVKLTKPLTEKDIIAERCGVR
                     PLVVTNTGDQTADWVKLSRKHEVEVNKSDRHISIFGGKLTDCINVGDEVAAFVKDLGI
                     DLPFFDQKWYGEPSEEIKNEFLHQAKLMGLDAMTNPSSSEPLSKRLWRRYGAQALGIL
                     DDIREKPDRAEILIEGTEYIRAELEEAARREMITKLEDFLRRRAKISLVVRKEELKKS
                     TGLKEACKILFGSEADKKWKEYFG"
     gene            8869..9630
                     /locus_tag="PLF66_16205"
     CDS             8869..9630
                     /locus_tag="PLF66_16205"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HMZ65636.1"
                     /translation="MNTWLQVLLFGFLGVSSIYSEDFYNYTVKDDDNSDKISREQLED
                     VKKWPDILNLNKLDPKKKIKPGTVIKIPSHLLKKKTSLVKVIAIDGVVKLKNEKTGEW
                     KDLKVNDEMSSGQLIVIREKGALEIESISIPGLVIQAREKSSFKLKSNLEVLELSSGE
                     IILSYIPRDRDSLKDLKILTPISTITFSGSDFTVKSEENLDRYTCSRGILNISNGNKS
                     ISLESGFGTIATDSFLQDPVKLLEKVIVKDPKISK"
     gene            9688..10128
                     /locus_tag="PLF66_16210"
     CDS             9688..10128
                     /locus_tag="PLF66_16210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65637.1"
                     /translation="MNIESIKSEIRNKNYELAIQMMDQTPSENRNSEYYLLRGICLQV
                     ANPLGFDIQDAESSLKKSVELEPNNKDAKLELAYFLLNVKHDPSSAKKYFLDVLKITR
                     DEVPESIATIMEHTKELKETYSSFANTLKEQAEIVLSSDNNNLE"
     gene            complement(10170..10517)
                     /locus_tag="PLF66_16215"
     CDS             complement(10170..10517)
                     /locus_tag="PLF66_16215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65638.1"
                     /translation="MKKILLVFVCFFFAANVYSEKSECRQKAEETFKTEREACKDKKG
                     DEKEKCLTDAKTKKKSSIEECQATHKACMEKARSEKQTAMDGCKEKKGEEKKECRSAA
                     DKKFEEARDACKK"
     gene            complement(10718..11836)
                     /locus_tag="PLF66_16220"
     CDS             complement(10718..11836)
                     /locus_tag="PLF66_16220"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF020976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004035 - alkaline phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HMZ65639.1"
                     /translation="MSLLKKILPIAYFVSVLENLYYIKKNLSYSYKALLLLLVYLNSS
                     KIYSEESKQIIQKIGFGSCLEQWNPQDIWKPMIESKPDLFIWLGDNIYFDSYIAEEKK
                     YYYNLLTNRKEYKELKKTSRMLYTWDDHDYGINDSGAEYEDKVNSQRIFLEAFEEKKD
                     SPRWTRPGVYDSYYFGSKGKRLQVIVLDTRYFRSELKRDWRKIFGIKRNIANNDPGAT
                     ILGESQWKWLEKELEKEADLRIIVSSIQIVNNYHPFEKWGNFPAERKRFFDLIKEKKA
                     KRLIVLSGDRHMSELSVEKENLPYPIYDFTSSSFNKPFKFIKPEESPVRIGDAIVEEN
                     FGTLDIHWDAKDPFIEMKIISPSGKQEFYHKIGFKEIE"
     gene            complement(11951..13828)
                     /locus_tag="PLF66_16225"
     CDS             complement(11951..13828)
                     /locus_tag="PLF66_16225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012388861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HMZ65640.1"
                     /translation="MGNFKTLNEMFFSINSNTGNVPTFFSKDTNKKFQGMTFRDAYTH
                     GENVGLYLMSLGLNPGDKVGLMADNRVEWAIADMGVLLNGAANVPRGSDSTPQEIQYI
                     LEHSESKFCFIEHEKLLESVLPIIPKTSVSKIIVLDKDYKSKYDNVINIYEAIEKGKE
                     LRATKLTELLKRARDTKEDDLFTIIYTSGTTGLPKGVMLTHKNMIYNVKSIPPLVGLK
                     KGDRVLSILPVWHIFERANDYSMISSGAAIYYTNVRDLRDDFLKVKPTFMASAPRLWE
                     NLYAGIKAKVEKSEPIRQLLFNTAYEVGKNFKKGLDYIQGNELQTKEEDGMEKLGKTV
                     QSLVTTANLAIPSIALDKIVFEKIREALGGELRGTISGGGALPAHVDEFFNVIGIPVY
                     EGYGMTECAPVISVRCLGKVIQGSVGFTPEGTDVKVLNDKGQEVKTGELGVIHVRGPQ
                     VMKGYYKNPEATAKTIVNGWLNTGDLGFKSFNGTLSVRGRVKDTVVLLGGENVEPVPI
                     ENLLLEHPHINQVIVVGQDQKSLSCLIWPDVEKLKEAGWNISFGEDLNKNEKLRAHYM
                     TAIKDIISGQNGFKSFERISDFRFLPKAMEVGDELTNLYKMKRNVISDKYAKLIKSMY
                     E"
     gene            14122..16092
                     /locus_tag="PLF66_16230"
     CDS             14122..16092
                     /locus_tag="PLF66_16230"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HMZ65641.1"
                     /translation="MKGFTITKYLHHCWGLVFFLFFHLSLLSEPIEVINETSYPLGKF
                     IYYFEDKSNEISFSDVLAGKYEANFEKSRSESPNFGFKDSSYWAKIEIENKGLPQKDF
                     FIEQAFPPIDFFEIYYQENSDWKNIQLGDSFPFYHRIYTHRNFIIPVEIPTHTKKTFY
                     FKMKTKSVSVFRLILYSTEAFHTRTEEYNFFYGFFYGILIILFIKNIFLFIILREKIY
                     SYYVLFVFGIGMYFWVFNGFAFQYFWPNQMNWNDLSNPFFMTLAIFGAAKTSIHFLDI
                     VHYSPRARIVMDALAYLTLLNTLLVFIFPYKYVLYYLYIMITPVAVSIFIAAIISYLR
                     KNPMAKFFLTGYISLIVSAFLVALNNLGIRSSYASPDLLQGASCFAICILSLGLSNRY
                     YVLRQQKLELESKAFQINTRLNRIENELEIAKKIHESLLPSVVSEILNLRLSAKYISS
                     SEISGDFYDFHSLEGKHLGVFIADVTGHGVPAALFSSIVKFAFSREWENMKEPSRLLS
                     NMNTSLFEKIGNNLLSAGYLYIDQISRRITYASCGHPPLLIWKKEDKEFIELKPKGRL
                     IGISREIVLFDSILKLEEGDRILFYTDGLWECENEKGIPFGETALQRFTAEKENLKAE
                     EFANSLIETLQDYSKSSGNFSDDVTFIVIDIL"
     gene            complement(16087..16863)
                     /gene="rlmB"
                     /locus_tag="PLF66_16235"
     CDS             complement(16087..16863)
                     /gene="rlmB"
                     /locus_tag="PLF66_16235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004472508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HMZ65642.1"
                     /translation="MEKKVNRGAIYGKRNVAELIHKCHEENLPAAKWGFREVFVKKQP
                     SREIIEDILNFIPKGIKITYLNGAELDAMFKGVNHQGIILIKEDSRKSNYSGDFSGLQ
                     ELVAEERGPILILDRIQDTGNLGNILRTAECFGITRIVLSERESAPVNDTVERISSGA
                     IHHLKIFRVVNLRQVLDFLKENGYWIVATSDRGLDSWDKLPELDETAIIMGNEEAGVK
                     RILLENADFVFQIPMHGKVSSLNVVVATGIVLDRIVNRNL"
     gene            complement(16848..18257)
                     /gene="cysS"
                     /locus_tag="PLF66_16240"
     CDS             complement(16848..18257)
                     /gene="cysS"
                     /locus_tag="PLF66_16240"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000570123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HMZ65643.1"
                     /translation="MLEVKLYNSYTNKKEVFKPENPNSVKIYSCGPTVYNFNHIGNFR
                     SYIFTDVLRRSLKLLGYQLDQTMNITDIDDKIINESIKLGVTVEEFTKKWIQIFFEDL
                     ATLNIEKLEHYPKATESVPEMIDLIDSLKEKKLIYEKEGSIYYSLAGFKNYGKLSKID
                     IDGMKTGARYDADEYTKDDVRDFVLWKSPKLEGEKFWETKYGNGRPGWHLECSAMIRK
                     IYSSGIDIHTGGIDLLFPHHENEIAQSMGAYPDEHFVNLWMHCEHLLVDNQKMSKSLG
                     NYYTLRDLISKGYDPKAIRYLLLSAHYRTKLNFSLKSLEEASKAIAKIQNTLTRILEK
                     LNYDLSLSKQTGYSKKQLEEFLESIADDLNTSKAVGTIFDFIKTINASLDSNSLSKED
                     AGDTLFYFQKINDLLGILNFEKPKEENLDSEIQALIDKRQEAKKNKDFALADSIRKEL
                     LDKGIILEDSPTGVKWKRK"
     gene            complement(18487..19335)
                     /locus_tag="PLF66_16245"
     CDS             complement(18487..19335)
                     /locus_tag="PLF66_16245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydrofolate dehydrogenase/cyclohydrolase
                     catalytic domain-containing protein"
                     /protein_id="HMZ65644.1"
                     /translation="MSSPIVLDGKKLSEKIKANIQLEIQKLKESHNFVPTLATILVGN
                     DPSSKVYVKMKMNACEKLGMKSKLIELPETTTTEELLSVIDSLNADISVTGILLQHPV
                     PSQIDERLAFDRISEKKDVDGVNSLSFGKLSMGEKTFYPCTPYGIILLLEEYGINPTG
                     KHAVIVGRSPILGKPMAMLLLEKNATVTICHSKTKNLPDFVRQADIVVGAVGKPEFIK
                     ADWLKEGVVLIDAGYNPGNIGDIDLKNSISKSTYYTPVPGGVGPMTISVLLLQTMLAA
                     KEKFSN"
     gene            complement(19683..19946)
                     /gene="rpmA"
                     /locus_tag="PLF66_16250"
     CDS             complement(19683..19946)
                     /gene="rpmA"
                     /locus_tag="PLF66_16250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010570657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HMZ65645.1"
                     /translation="MAHKKGGGSSKNGRDSRSQRLGLKKSGGQLVKAGNILMRQRGTV
                     FRAGTNVGIGRDHTLFALVDGIVTFGYFDKKRKQISIVPKAVA"
     gene            complement(19949..20266)
                     /locus_tag="PLF66_16255"
     CDS             complement(19949..20266)
                     /locus_tag="PLF66_16255"
                     /inference="COORDINATES: protein motif:HMM:NF016233.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HMZ65646.1"
                     /translation="MILIEIRKDQEGNYTGLKSEGHAPDTFGSKGNNILCAAISTLVQ
                     TLHLFLKIMSNVSHETSRTGFLNFDVNNTGKDTNLAFELVLTGIHNLSKQYPEEVQVL
                     IRS"
     gene            complement(20263..20571)
                     /gene="rplU"
                     /locus_tag="PLF66_16260"
     CDS             complement(20263..20571)
                     /gene="rplU"
                     /locus_tag="PLF66_16260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012388757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HMZ65647.1"
                     /translation="MYAVISLSNQQFKVQKDQVFLAQKSDKPVGTEFDAQILLTAQEN
                     KVNIGSPVVSGAKVTLKVLEDVRGEKIHGFKYKRRKNNHRRWGHRQDLQKFQVTNLVL
                     "
     gene            20676..22478
                     /gene="typA"
                     /locus_tag="PLF66_16265"
     CDS             20676..22478
                     /gene="typA"
                     /locus_tag="PLF66_16265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HMZ65648.1"
                     /translation="MEIRNICIIAHVDHGKTTLLDGILKETGAITSKENRERMMDSND
                     LEQERGITIIAKNTAVHYKGTRINIIDTPGHSDFGGEVERVLNMANCSLLLVDAFDGP
                     MPQTRFVLGKALQLGHRPILVVNKVDRDGARPNKVVDMVFDLFHDLNATDEQMDFPII
                     FASAKLGWAVNDISEVPGKNLEPLLDLVLKHVQLSKGDVTAPLQFQVTSLDYNDYVGR
                     IAIGKIYQGTIRRGQDITLVTMKDGKTSNHKISKLYGFEGLKRHEIESAEAGDIVALA
                     GVPELFIGDTVCDLGQPIALPPITVDEPTVSMFFMVNNSPFVGKEGKLVTTRNIRERL
                     DRELQVNVAMRLEETDDKDRFKILGRGELHLSILIENMRREGYELQVSRPEVIYKQAE
                     DGSKLEPYETLVMDLPDQYTGQIISELNRRKGEMTHMDAHESGITRVEYIIPTRGLIG
                     FRSYFISETRGEGVSTSRFLHYGPYKGEIPGRKNGALISMDSGDTTAYSLWKIQERGV
                     LFIDPVIPVYPGMIIGEHSRENDLEVNPCKGKKLTNVRASGSDEAIRLVPPRKLSLEQ
                     SIEFLDDDELLEVTPLSLRLRKKILDGTQRKRVK"
     gene            complement(23072..23647)
                     /gene="cobC"
                     /locus_tag="PLF66_16270"
     CDS             complement(23072..23647)
                     /gene="cobC"
                     /locus_tag="PLF66_16270"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043755 - alpha-ribazole phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ribazole phosphatase"
                     /protein_id="HMZ65649.1"
                     /translation="MEIYLIRHTKVAVPSNICYGQTDVSLANSYQKEIKQVLSNLGST
                     ENFTCYSSPLERCRVLANSISTSPILEKRLMEMNFGDWENKSWDEIGKTEFDQWHSDF
                     VKNHVPNGESYFELYTRSISFLNEILLENKNSILVTHGGVIRAILAHTLGLPLENSFR
                     LKIEYGSISKIQVTNQISQVEFVNISADYHR"
     gene            complement(23632..24390)
                     /locus_tag="PLF66_16275"
     CDS             complement(23632..24390)
                     /locus_tag="PLF66_16275"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HMZ65650.1"
                     /translation="MKRELELFFTALMFFTRLPVPEINYSQEKLNASSRYFPLVGILV
                     GTLTAIAFMFSSIVFPFKIAILLSMTASILITGAFHEDGFADTCDGFGGGYTKEKILE
                     IMQDSRIGTFGTIGLVLILGTKFILLSEIPAMIVPIAIVSGHAISRLISISLLYSMVY
                     VKAEGKAKPLSTALPLGSLIVASLFGLLPLLLFKKFFIFLVILPLLLTRSFLSYYFRK
                     HIGGYTGDCLGAAQQVSEIIFYITVYLFAQWKFI"
     gene            complement(24387..25424)
                     /gene="cobT"
                     /locus_tag="PLF66_16280"
     CDS             complement(24387..25424)
                     /gene="cobT"
                     /locus_tag="PLF66_16280"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002151323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HMZ65651.1"
                     /translation="MLKKFSVLKPDQSILNELAEEINNKTKPPGSLGRLEDIATQIGL
                     IQGTTTPKLKKPTIVIFAADHGLAKEGVSPYPQEVTYQMVMNFLHGGAAINAFCEENQ
                     LDLKIVDSGVNYDFEGAEGMIHAKVGFGTKNILQEEAMTDQELESAIEHGASIVSNLS
                     EQGCNVIGFGEMGIGNTAVSSLLISKLLNLPIENCVGRGASGNDDHVKKKKDLLKKAS
                     QVHNTSDAVSILKAYGGFEIAMMIGAILQAAENKMLILIDGFIVTTSLLFAKTLYKEV
                     INYCIFSHLSGEKAHEMIVNYLGGKPILSLGMRLGEGTGAALAYPIVSASVKFLNDMA
                     SFEEAGVKNKS"
     gene            complement(25513..>25749)
                     /locus_tag="PLF66_16285"
     CDS             complement(25513..>25749)
                     /locus_tag="PLF66_16285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65652.1"
                     /translation="VKAANNHNPLRSEQTLLSKILSFNESTDHAGTYECRPLNTKNVR
                     DADDIWEECECILYGFYPNGLTGLSQEEFPNNNN"
BASE COUNT         8307 a         4228 c         4560 g         8654 t
ORIGIN      
        1 ttccaatgtc tccatcgaat atgtgcaact aagagtgaaa ttttttgaag gagatacagt
       61 agaatctctt cgggaccttg gtgtgaattg ctacaaagct cattataatg gaaaggtatt
      121 ttctccaatg aaatcattga ttcaagcttt aaaacagaat ggttttgagg tatggatcat
      181 aacggcaagt ccagaagcaa tgtatcaaaa aatacttagt gaggaattgc aaattccaat
      241 tacaaatatt gttggagtaa aatccattgt tagagaagga aaaattacag gtgaaatgat
      301 tttacctatt ccacaagatc atggaaagaa agaggcaatc gagacttttg tgcaagagag
      361 acctttactc gttggcggaa atagcagggg tgacaaagaa atgattgaat atagctccaa
      421 cttgaaactt atcattaatc cagatgaata cgtagcagaa gatcaaacag aaagtattgc
      481 cgaatatgct agaaagaata attgggtcat tgcaaaagta agagacgtgc cggctaacga
      541 ctttagttat atttcctcta aagattttaa tatcaaaaaa aataaaacaa atgaataatt
      601 catttcaaaa taaactactt tataggaata taaaatgatt ctaaccgaat tctttaaacc
      661 agcacctcct aaagaaaggc ttcccgaaaa ggagattgaa tacaaatatc caaagcttag
      721 atggcaagtt ttagagtcca cttttcttgg atatgccgct tattatttcg ttcgaaacaa
      781 tctatctgtt gtttcaaagg aaatgggaga ggcgctacag tatgataaat cccaaattgg
      841 tgacatactg gcagtcaccg caattaccta tggtataggc aaatttgtta tgggtgctct
      901 ctcagataga agtgattctc gtaaattcat ggctgtgggg cttttaataa ctgctggtat
      961 taattttatt tttggaacag tagaaaatta ttttttccat ttatttttat ggggactaaa
     1021 tggttttgtg caaggaatgg gctggcctcc ttgtgggaga tcaatagggc attggtttag
     1081 tttaaaagaa agaggaagcg tgtttgctat ttggaacgtt gcgcacaacg taggtggagg
     1141 gatggttgga gtggttgcag cattttctgc gagtcattgg ggatggcgct ctgctttcta
     1201 tgttccgggg atactcgctg ttatctcttc tgtttatatt tttattcgac tcagagatac
     1261 tccacaatca gaaggtcttc ctcctattga agaatataaa aaagacaatc catctaaaca
     1321 attgaccgag gattctgaaa gagaattaga aactaaggaa cttttaacaa agtatatttt
     1381 taataataaa ttcctttggc tatttgcaat tgctaacttt tttgtttacg ttgttcgcta
     1441 tagtatgtta gattggggtc ctacctattt aaaggaaatg aagggtgcaa gtcttacgag
     1501 tgggggaatg agtgttctaa ttttagaatt tgcaggcatt ggtagcacaa ttttaatggg
     1561 ttgggtatcg gatcgacttg gtggaagaag aggaatggtg agtcttcttt gtatgattcc
     1621 aatctttttt gcttttgcgg ctatttacta taatccgccg ggaaatctct ggattgattt
     1681 tactatgctt ggtatcattg gattttttgt ttatcctcct gtcatgttac ttggtgttgc
     1741 ctctttagat gtaacttcaa aaaaagctgt tggaacggct gctggatttg taggactctt
     1801 tggttatatt ggaagaactg ctcaggcaaa gggtttgggt tggctagcca atcattctga
     1861 atatggttgg aattatgttt tatactcaat cctaatttgc accattttat ccattttatt
     1921 tcttgggttt acttggaata taaaaccaaa aagataaaat tttctgtgat ccataaaatt
     1981 tgaaactgaa tattagatac ttctaatatt cagcaaattt gaattgcaaa tttattgctc
     2041 tctaatagta tttcccgaaa ttttaaataa agcaaaagaa aaccgattct ataactcatc
     2101 ttacgcaaaa tttaaaattt gaggaataaa tgatgatttt taaaaaatta gagaatacta
     2161 atgaattaat aaaatcaata acctgcggca aagctcggct gaatgagagc gaaaaagtga
     2221 tgggaggaaa gcctttcgtt ccctctcttt cgcacagctc aagcctattg tcatttttca
     2281 atgacaatag gagcaaacgc atggttcacc aaaactcaat cattgctttt gttaagcaat
     2341 gatactgtaa ggtgagttta caatttaaaa attagaatta tttgaggata tagctatggc
     2401 aactgtaaca gaagagctga tttatgagta ttgggattgc gataaatgtg ggcaaaaaga
     2461 aattaggggc gatataagaa cctgtcctaa ttgcggaaat gcaagaaatg aaaatataaa
     2521 attctatcga atcgaaggga aagaagagaa ggtaacagat aaagcacagg cagataaatt
     2581 taaagcaggt gcagattggc tttgttcttt ttgtgaaacc ttaaattctg ttacagacaa
     2641 acaatgtctt tcttgcggtg cttctcaaga ggcgagtaag aaaaattatt ttgaagtcca
     2701 aaaggaaaaa gaaaaaaaga aagaaaaaaa taaaccaaat caaaaaccac aagagcaacc
     2761 taccaaaaag ccttttaatt ggaaaaagtt tagtgtttgg gcattgactt tacttggggg
     2821 ctgtattgcc tctatttatt ttttaagtag aacacataac gtaacatttc aaatcgtaga
     2881 cgtttcttgg gaaagaaaag ttcccgtctt aagatatacc aatgttcaga aaagcgattg
     2941 ggatgaccaa ttatctggtg atgatattgt aaaaatttct tctgcacaag aagttcgatc
     3001 gtacgaagac aggcaagtag gaactaaaac agagcgatac acggaaacgg aacaatacca
     3061 atctggttct cgtagagaat gtcgtactag ttatgaatca acaggatcag gagcctctaa
     3121 aaaggttact agttgtgata gcgttccaac ctattcaagt agaaccgtta caaaaacgag
     3181 agaagttcct gtgtataaaa aatttcccat ttacggaaca aaagtaactt atacagcaaa
     3241 tgagtataaa tttttacgag aagatagttt atcaggaaaa gacaatgaac ccaaatggcc
     3301 tgatgttaaa ttggggatag gcataaagaa taaagaagat ttaaaagggg agccaacggc
     3361 tacttatatt gtagaattta aaaaagtaaa tccagaagac caagcaaaag attttcaaaa
     3421 gattatgacg acagaagata agtttaggaa tgtatatata ctgaatcaat ctgtgattaa
     3481 acaagtaaat atatttgatg aaattgttct cgaagatggg gaagagagaa tagaagagaa
     3541 aaaagaatga ttgcaaacct ggaattcaaa aaaacgaatt ttaaagataa aataatctct
     3601 gttttacaaa gcgatgttcg gattgtacag attcttctta tgggaactct tttaagtatt
     3661 ggtgtgtatt tcagagactt ttcaattcat ccaatgcaaa tttttctaac tttttgctct
     3721 ggtttagcca ctcatttttt atggaatcga attttaaaga tcaaagtagc cattctaagc
     3781 actgtgataa cttgttttgg tttatctctt ttgcttagaa gtgatttgtt ctggattcat
     3841 cctgcgattg tgtttctagc tatatcctca aagtttatca ttcgaatccg cggcaaacac
     3901 ttatttaacc cagccatgtt tggagtcatt ctaggaatta gtttgtttcc tagaacatgg
     3961 gtttctcctg ggcagtgggg atttgagtta acaattggaa tttggcttct tgtgtttggg
     4021 ctcatagttt ccggaagagc tggtatttcg gatatcagct atgccttttt attatttcat
     4081 tttctttttc tcgcctatcg tatttttcgt tttggatacc agtgggatgt gctactccat
     4141 caatttcaaa atggagcgct gattctattt gcttttttta tgattacaga tcctaagacg
     4201 attcccgatc ataaaatcgg tcgaatcctg catgcatttc tagtcgcaat gttttcctat
     4261 atttgggcat ttgttttttt taaaacgaat ccgtatattt gggcattgtt tatttcttct
     4321 cctgttgtga ttttttggga ttatttactg aaagcagaaa aatacaaatg gaaattagag
     4381 aactaaaatt cgatgcataa ttaacatcag ttcgatgtaa aaatattttc accaccgact
     4441 tgcactgagc cgtgtcgaat gtgtgtacac cgatgactga acgatacgga aacgatggat
     4501 acttttgaat tatcgtatat ttgatctgtg gtaatctttt gaattttttg ttacattgaa
     4561 ctggcgtgaa tttttaataa ccacgaagcg cacgaagacc cacgaagtga ataaataata
     4621 aatcttctct tttcatcgtg ttctctgtgc ttttcgtggt gaattctttt tgatttctct
     4681 taacttgaaa gcattaagcg acagtctccc tgtctctcat cttacgcgcg aatgtgtaag
     4741 gtgagttatt cataaactct attctcgttc aaataggcaa gaaattaaaa ggctggaact
     4801 ctcacttcta tttttccact aaactctgta actggaattt catattcttt tgttttcagg
     4861 aaataacgcc cgttctcctc ttctaaaaga ttcgaaataa aatcatgcga ttttctgttt
     4921 aatttaaatt tgataaactg atcctttttt cgcatacaaa aaagtttctc ttctttatcc
     4981 gaatagaaat caaaatcttt caaaggagta atggattcat cttctgcttg taagaaaata
     5041 ccttctgatg tttcgattgc attttcaatt tttaaaaaaa tacagtttgc ttctaaatat
     5101 ccatgctcca ctttgtctgc gtaagaattt gtgatataga ttcctttatc atcctcgcag
     5161 atattcttct taaaaaaaga gagaacattt tcctgggtta tttcttgacc tctaaaaagc
     5221 caacgaccag tttctgttat tttaatttcg ctatcgaact ttcttgggat tattgtcatt
     5281 tattttccta tatctatatg ggtagccaac aagctttccc atttctcgat ttgcttctgt
     5341 ataagcaaca gtcattcctt ctccattttg agtgtttata atcagaggag gtattgtttc
     5401 taatgttttt ttgtaaatcc atactccgtc aatttttcta cgaaacattt ccatttgaag
     5461 tgtataaagc tctgatttca tgtcaattgg atctacgaag tttctaaata tgactgttcc
     5521 caccattttg aaatctcgta ctggtctttc tgctactatt tcaacctctt caggtttttg
     5581 cttagaataa atgggatttt cttctgtaaa atcagaatcg ggctgaaaat cgacactgct
     5641 tgcacaattg aataccaata aaattagtaa gattgaaaaa gagattctca tacttagaac
     5701 atactttttt tttaacttaa gttcaatagc attttttcgg ggataaatca aaagtaaaaa
     5761 taggctagat tatgatttac atttttaaaa actaaaaaat tgtattcttt atatgagaaa
     5821 ttatgcaaag aaagtccaac tattcatttg ctttttaata tcttttaatc tttgctctca
     5881 atcgaaagag cctttatcta tagatgggaa agttttagat aaaaaagaga aaaactgttc
     5941 ctataagatt aaatatccaa tcttaaaatt aggaaaagat tctaaatacg atgtaagcgt
     6001 cgtacgtaat attggaaaag ttttcataga tgaatttaca aacattgaaa aatatgaaaa
     6061 cgagtttgaa tgtaataaga aagataaaaa tgcaagtcca tttactttaa atggagattt
     6121 cactgttaaa ctcaaaacag attccattct aagtatacat tctagttttt cttcttatgt
     6181 tgaaggtaat ccttatccaa acaatgtatt taaaacatat aactttgaac taaattcagg
     6241 aaaagagatc gcatttgaag atctttttaa aaaggattcc aagtatttaa ttcctttgca
     6301 taagtttatg gcggaagcta tgctaaaaga aaaagtgatt agtgagaaag atgaatttat
     6361 cgccttaaaa aaagaaaagt atgattttta tttaactcaa aatgctatcg tatttataaa
     6421 tctatttgat atacatgcaa tgcaatctgt ggaagtaatg attccatatg ataaaatgaa
     6481 aaaatatcta aacaccgata tggtattgaa gtttgtaaaa tgaataagga tcaagtttta
     6541 gaagcttttc aaaaatcggg taggcaatta tcaaaagatg ttttatttga aattcgagac
     6601 aattcaaaaa aatacaaaaa agaattaatc aaaatactct cgaaagaagc caaaaagccg
     6661 caagatccaa tttcgaaatt tgaacagaat gactggattt actctatgta tttgttagcg
     6721 gagtttagag aaaaagaagc actttcccct atattaaaat tattctcaag aaaaggaaat
     6781 tttttgaatc cattgactgg agatattgtt ttaaccaatt tggggcaaat tatcgcatct
     6841 gttgcgaatg gggaaaatga tgagcttaag aaattagttt tagatactag tttgaatgaa
     6901 tactgcagag cggctttttt agaagcgtta gttgttatga ctgagttaaa tgaaatggat
     6961 cgggaagact tgtatcatta tttcaaagat ctattcaaaa atttggaaaa ggaacctata
     7021 tttccttggt cggttcttgc acaatgttct gttttacttt ttaataaatc atttctaaag
     7081 gaaatcaaac aagcttataa aaacaattta atcgatgaaa tctatattga tctagaggaa
     7141 atcaattctt atatatccga tccagaatta tctcaactag gtcttgcaaa gaaaaaatat
     7201 ggaatcatta aagatgcgat cacagcaatg gattggtggg caaattataa agaggaaaaa
     7261 gttgtatgaa attaagagag agtaatattg caaagttaca gaaagaaaat ttcgatgtta
     7321 ttattttagg cggtggaatt aatggagcag tttcagcggc tgcgttagct gctaaaggag
     7381 cgaaagttgc gttaattgat aagggtgact ttgctggatt tacaagtcaa aattcttcca
     7441 acctagcttg gggtggaatc aagtatatgg agacttttga atttgggctt gttcgcaaac
     7501 tttgcatgtc aagaaataaa ttgatgcgta attatccatc caccgttgaa gaaatccgtt
     7561 tttttactac aattacaaaa ggatttcgac atagtgtttt gaaattatat gcagggacct
     7621 tattctattg gttaatggga aatttcttca caaagccacc tcgctatcta tccaaaggta
     7681 gaatcaacca agaggaagat attattaatt tagagaatgc agtaggtgga tttgaatatt
     7741 cagatgcata tttacatgat aatgatgcta gattcgtatt tctttttatt cgctctgctt
     7801 tgaactatgg atgtattgcg gctaattatg ttgaatcaac tggtgcatct aaagtcggag
     7861 atttttggaa tgtgaaagca aaggataaca tttctggaaa aacatttacg cttcgtggga
     7921 aagttctaat taattcctgt ggaccttttg tagatgagca caaccagcta acaaatcaaa
     7981 agacaaatca tagacatgta tttagtaaag gcattcattt aattgtagat cgacttacag
     8041 aaacaaaaca tattttaact ttttttgcag atgatgggcg gctatttttt gttatcccaa
     8101 tgggaaatag aacttgcatt ggaacaacgg atacacaagt aacttcccct tatgcagaag
     8161 taacaaaaga agatagagaa tttgtgttgg cgaatatcaa caagagagta aaattaacaa
     8221 agcctttaac agaaaaggat ataattgcag aaagatgtgg agtaagaccg ttagtggtaa
     8281 caaatacagg cgatcagact gcagattggg taaagctttc aagaaagcat gaagttgaag
     8341 taaataaatc tgataggcat attagtatct tcggtggtaa actaacagac tgtatcaatg
     8401 taggagatga agtagcagcc tttgtaaaag atttaggaat tgatctacct tttttcgatc
     8461 agaaatggta tggtgagcca tctgaggaaa ttaaaaatga atttttacat caagccaaat
     8521 taatgggact ggatgctatg acgaatccaa gttcgagtga accattatcc aaaagacttt
     8581 ggagacgtta tggggcacag gctcttggaa tcttagatga tataagagag aaaccagata
     8641 gagccgaaat attaattgaa ggaacggaat acattcgagc cgaattggaa gaagctgcaa
     8701 gaagagaaat gatcactaaa ttagaagatt ttcttagaag aagagctaaa atttctctag
     8761 ttgtaagaaa agaagaatta aagaaatcca ctggattgaa agaagcatgt aagattttat
     8821 ttggaagtga agcagataaa aaatggaagg aatactttgg ataaatatat gaatacttgg
     8881 ttacaagttc tactgtttgg attcttggga gtttctagca tttattcaga agacttttat
     8941 aattatacag tcaaagacga tgacaattct gataaaattt ctagagagca actagaagat
     9001 gtaaaaaagt ggccggatat tttaaattta aacaaactag atccaaagaa aaaaataaaa
     9061 ccagggactg tgataaaaat tccaagccac ctactgaaaa agaaaactag tttagttaaa
     9121 gttatcgcaa tagatggcgt agttaaacta aagaatgaaa aaactgggga atggaaagac
     9181 ttgaaagtta acgatgaaat gagctcagga caattgattg tgatccgaga gaagggtgct
     9241 ttagaaatcg aatccatttc cattccagga cttgtaattc aagcgagaga gaaaagtagt
     9301 tttaaattaa aatctaattt agaagtatta gagttaagtt ctggggaaat aatcttgagt
     9361 tatataccaa gagatagaga tagtttaaaa gacctaaaga ttctaacacc gatttccact
     9421 attacttttt cgggaagcga tttcactgtg aaatcagaag agaatttaga ccgttatacc
     9481 tgttctaggg ggattctaaa tatctccaat ggaaataaat ctatttcttt agagtcagga
     9541 tttggaacta tcgcaacaga ttctttttta caagatccag taaaattatt agaaaaggtg
     9601 attgtgaaag accctaagat atctaaataa tcttttcgtg cttgcttttt taaatttaat
     9661 tcataagatt gctatcccga gatatatatg aatattgaaa gtattaaatc tgagattcga
     9721 aataaaaact atgaacttgc catacaaatg atggaccaga ccccatccga gaacaggaat
     9781 agtgaatatt atctattaag aggtatatgc ttacaagttg cgaatcctct tggctttgat
     9841 attcaagatg cagagtcttc tcttaagaaa tcggttgagc tagaacccaa taataaagat
     9901 gccaagctag agcttgccta ttttctgtta aatgtaaaac atgatccatc ctctgcaaaa
     9961 aaatacttct tagatgtact gaaaattaca agggatgaag ttcctgagtc gattgctacg
    10021 attatggaac atactaaaga actaaaggaa acttattcct cttttgcgaa cacattaaaa
    10081 gaacaagccg aaattgtttt atcgagtgat aataataatt tggaataaaa aagagcggat
    10141 aataaaaatt aaccgctctt tttaggagtt tattttttac aagcgtctct tgcttcttca
    10201 aacttcttgt ctgccgcaga gcgacattct tttttctctt ctcctttttt ctctttacat
    10261 ccgtccatag cagtttgttt ttcggaacga gctttttcca tacaagcttt gtgagtagct
    10321 tgacattcct caatgctaga tttctttttt gtttttgcat ctgttaaaca tttttctttt
    10381 tcatcgcctt ttttgtcttt gcaagcttct ctttctgttt tgaaagtttc ttctgctttt
    10441 tggcggcatt cagatttttc tgaatacaca ttcgcagcaa agaagaaaca tacgaatact
    10501 aataaaattt ttttcattct aagtctccta tattaaacgt tagttcgatg taaacatttt
    10561 tcaccaccga tgaacaccga taactgaacg ataaaggcgc gatggataat tttgaatcat
    10621 cgtatctttt atcggtggta atcttttaat ttttctgtta cgtcgaactg gctttaattt
    10681 atataaaata atgtagagta ttaatgcaac tgaatttcta ttcaatttct ttaaatccta
    10741 ttttgtggta aaattcttgt ttgccgctag ggctgatgat tttcatttca ataaatggat
    10801 cctttgcatc ccagtgaata tcgagtgttc caaaattttc ctctacgatt gcatccccaa
    10861 ttcttacagg ggattcttct ggtttgatga atttaaaggg tttattaaaa gagctagatg
    10921 taaaatcata aatcgggtag ggaaggtttt ctttttctac acttagctct gacatatgac
    10981 ggtctccgct tagaacaatc aatctcttcg cctttttttc tttgattaga tcaaagaatc
    11041 tttttctctc agctggaaaa tttccccatt tttcgaaagg gtgataatta ttcactattt
    11101 gaatacttga tacaataatc cttaaatcag cttctttttc taattctttt tccaaccatt
    11161 tccattgtga ttctcctaag atggttgctc caggatcgtt atttgctata tttcttttta
    11221 ttccaaagat ttttctccaa tctcttttta attcagagcg aaaatatcta gtgtcaagta
    11281 cgatcacttg aagtcttttt ccctttgaac caaaatagta agagtcataa acacctggtc
    11341 ttgtccatct tggggagtct tttttttctt caaaagcttc tagaaatatt ctctgcgaat
    11401 tcactttgtc ttcatattcg gcgccagaat cattgatccc ataatcatga tcatcccaag
    11461 tgtagagcat tctagaagtt tttttcaatt ccttatactc tttgcgattt gtgagtaggt
    11521 tgtaatagta ttttttttct tctgcaatat aggagtcaaa ataaatatta tctcccagcc
    11581 agataaataa atctggtttt gattcaatca ttggcttcca aatatcttgt ggattccatt
    11641 gttctaggca agagccgaaa ccgatttttt ggatgatttg tttgctttct tctgaataaa
    11701 tctttgaaga attgagatat acaagtaaga gtaagagcgc tttgtaggag taagataaat
    11761 ttttttttat atagtataaa ttttctaaaa cagaaacgaa gtaggctatg ggaaggattt
    11821 tttttaataa actcataaat ctatcatcta ggatagaaat aaaaaatgca agaggaaaaa
    11881 gatataaaaa aagccgagcg gctaactcgg cttatcctca gaatgaaacc tctttgagat
    11941 ttataatgat ttattcatac atgcttttaa ttaatttagc atatttgtca gaaataacat
    12001 ttcgtttcat tttgtatagg tttgtcaatt catcaccaac ttccatagct tttggaagga
    12061 atctgaaatc actgattctt tcaaagctct taaatccgtt ttgtcctgaa atgatatctt
    12121 tgatagctgt catatagtga gcgcgaagct tttcattttt atttaaatct tcaccaaagc
    12181 taatattcca acctgcttct tttaattttt caacatcagg ccagatgagg caagataaag
    12241 atttttgatc ttgtcctaca acaattacct gattgatatg aggatgttca agtaataagt
    12301 tttcgatagg gacaggctca acgttttctc caccaagtaa tactacagta tcttttactc
    12361 tacctcttac agacaacgtt ccattgaagc ttttaaaacc taagtcaccg gtattcaacc
    12421 aaccattcac aattgtttta gcagttgctt ctggattttt ataataccct ttcataactt
    12481 gtggtcctct tacatgaatg actccaagtt ctcccgtttt tacttcttgt cctttatcat
    12541 tcaaaacctt tacatcagtt ccttctggag taaaaccaac gctaccttga atcactttac
    12601 ctagacatcg gacagagatt actggagcgc attcagtcat accataacct tcataaacag
    12661 gtataccaat tacattgaaa aactcatcta cgtgagcagg aagagctcct ccacctgaga
    12721 ttgttcctct gagttctcca ccaagtgctt ctctgatttt ttcaaatacg attttatcta
    12781 aagctataga aggaattgca agattagctg ttgtaactaa cgactgaact gttttgccaa
    12841 gtttttccat tccatcttct tctttggttt ggagttcatt gccttgtata taatctaaac
    12901 cttttttgaa gtttttacca acttcataag cagtattaaa aagcaactga cggataggtt
    12961 ctgatttttc aactttagct ttaataccgg catagagatt ttcccaaagt cttggagctg
    13021 aagccataaa agttggtttc acttttaaaa agtcgtctct caagtctcga acgttagtgt
    13081 agtagatagc tgctccagag gaaatcatcg aatagtcatt tgctctttca aaaatatgcc
    13141 atacaggtag aatagataat actctatctc ctttttttaa gccgactaac ggtggaattg
    13201 atttaacatt gtaaatcata tttttatgag taagcattac accctttgga agtcctgttg
    13261 ttccagatgt atagatgatt gtaaagagat catcctcttt agtatctctt gctcttttta
    13321 aaagttctgt aagctttgta gctctgagtt cttttccctt ttcaatggct tcataaatat
    13381 tgattacgtt atcatatttt gatttgtaat ctttatcgag gacaatgatt tttgatacag
    13441 aagtttttgg aatgataggt agaacacttt ctagtaattt ttcatgttca ataaagcaga
    13501 atttgctttc actgtgttct aatatgtatt gaatctcttg cggtgtagag tctgaacctc
    13561 taggaacatt tgctgcacca tttaataaaa ctcccatatc tgcaattgcc cattcaactc
    13621 gattgtcagc catgagtccc actttatccc ctggatttaa acctaaggac attaaataaa
    13681 gaccaacgtt ttcaccgtgt gtgtaagcat ctcgaaaagt cattccctga aactttttat
    13741 ttgtatcctt tgaaaaaaaa gtcggaacgt ttccggtgtt tgagttgata gaaaagaaca
    13801 tttcgttcag agttttgaaa ttacccattc ttataattct cctgttttaa aatctatttc
    13861 gatttttatt agacgtttct aataattaaa tgaaaagaaa actaaaagca tatcagatat
    13921 tcaggttgtg ttgataaatg ttagaagctg atattttatc gatatggaac cgacatcttt
    13981 tagcataatc gcattgaatt caaattacag aattatttca acctaaaaat tgaatttcgc
    14041 aatggaaaca tcaaactgct ttacactcaa aagccttttt tataaatatg taatagcaac
    14101 caagagtgat tattaaagga aatgaaggga tttacgataa ctaaatatct ccaccattgt
    14161 tgggggcttg tgtttttttt attctttcac ctatcccttc tttctgagcc aatagaagtt
    14221 ataaatgaaa catcttatcc gctgggcaaa tttatctatt attttgaaga taaatcaaat
    14281 gaaatttcct tttcagatgt attagcggga aagtatgagg ctaatttcga aaaaagcagg
    14341 agtgaaagtc caaattttgg attcaaagat agcagctatt gggctaaaat tgaaattgaa
    14401 aataagggac tcccacaaaa agattttttt atagaacaag cctttccacc tatcgatttt
    14461 tttgaaattt actatcaaga aaattcggac tggaaaaata tacagctagg agacagtttt
    14521 ccattctatc atagaattta tactcatcga aatttcatca ttccagtaga aataccaacg
    14581 catacaaaaa aaacattcta ttttaaaatg aaaacaaaaa gtgtttctgt tttccgtttg
    14641 atactgtatt ctaccgaagc ctttcatact agaacagaag aatataattt cttttatggt
    14701 tttttttatg gcatccttat aatcttattc ataaaaaata tttttctatt tataatactc
    14761 agagaaaaaa tttactccta ttatgtattg ttcgtttttg ggatagggat gtatttttgg
    14821 gtgtttaatg gctttgcatt tcaatatttt tggccaaatc aaatgaattg gaatgacctt
    14881 tctaatccat tctttatgac acttgcgata tttggagctg cgaaaacatc gattcatttt
    14941 cttgatattg ttcattatag tcctagagct cgtattgtga tggatgcatt agcatattta
    15001 actctactca atacactctt agtatttatt ttcccttata aatatgtttt atattatctt
    15061 tatattatga taactcctgt tgccgtatcg atttttatag cagcaattat cagttattta
    15121 agaaaaaatc ctatggcgaa gttttttctg actgggtata taagtctgat tgttagtgcc
    15181 tttttagtag ctttaaataa tttggggatt cgctctagtt atgcatctcc cgatttatta
    15241 caaggagcat cttgctttgc tatctgtata ctatcgcttg gattatcaaa tagatactat
    15301 gtgctgagac aacaaaaatt agaattggaa tcgaaagctt tccagattaa tactaggtta
    15361 aatagaatcg aaaatgaatt agaaatcgca aagaaaattc acgaatctct tcttccatct
    15421 gttgtatctg aaatattaaa tttaagatta tctgcaaagt atatctcctc tagtgaaatc
    15481 agtggagatt tttatgactt ccattcctta gaaggtaagc atttaggagt atttattgcg
    15541 gatgtaactg gacatggagt tccagctgct ttattctcat ctatcgtaaa atttgcattt
    15601 tcgagagaat gggaaaatat gaaggagcct tctagattac tctcgaatat gaatacctcc
    15661 ttgttcgaaa aaattggaaa caatcttctc tctgcaggat atttgtatat tgaccaaata
    15721 agtcgtcgta taacgtatgc aagctgtggt catccgcctt tgcttatctg gaaaaaagaa
    15781 gacaaagagt ttattgagct aaaaccaaaa ggtcgattga ttggaatttc tcgtgagatc
    15841 gttttatttg atagcatttt aaagctagaa gagggcgatc gaattttatt ttatacagat
    15901 ggtttatggg aatgcgaaaa tgaaaaagga ataccgtttg gagaaactgc tttgcaaaga
    15961 tttactgcag aaaaagagaa tttgaaggcg gaggagtttg caaatagtct tatcgagaca
    16021 ttacaggatt attcaaaatc atccggaaat tttagcgatg atgtgacgtt tattgtaata
    16081 gatatcttat agatttcgat ttacgattct atccaataca attccagttg caacgactac
    16141 attgagagag gaaacttttc catgcattgg gatttgaaat acaaaatctg cattttcaag
    16201 tagaattctt tttacacctg cttcttcatt tcccataata atcgcagttt catctaattc
    16261 tggaagctta tcccaactgt ctagtcctct gtcgcttgtc gcaacgatcc agtatccgtt
    16321 ttcttttaga aagtctagaa cttgtcttag gtttacaact ctaaatattt ttagatgatg
    16381 aatcgctcca gacgaaatcc gctctactgt atcatttaca ggcgcgcttt ctctttctga
    16441 gagaactatc ctagtgattc caaagcattc agctgttctt aaaatatttc ctaggtttcc
    16501 tgtgtcttgg attctatcta gaattaggat aggacctctt tcttccgcaa ctaactcttg
    16561 taagccgcta aaatcgcccg aataattgga ttttctgcta tcctctttga taagtataat
    16621 tccttggtga ttgactcctt tgaacatggc gtccaattct gctccattta aataagtaat
    16681 ctttatccct tttggtatga agtttagaat atcttcaatt atttcgcgtg acggttgctt
    16741 ttttacaaag acttctctaa aaccccattt tgcagcagga agattttctt catggcattt
    16801 atgaatgagt tcggctacgt ttcttttgcc gtagatagct cctctattta ctttcttttc
    16861 cattttacac cagtaggact gtcttctaaa atgatacctt tgtccaaaag ttctttacga
    16921 atggaatcag caagtgcaaa gtctttattt ttcttagctt cttgacgctt atcgattaac
    16981 gcttgaattt ctgagtctag attctcttct tttggttttt caaaatttaa aattcccaaa
    17041 agatcattaa ttttttggaa atagaagagg gtatcgcctg catcttcttt tgaaagagaa
    17101 ttgctatcta aactagcatt gattgttttt ataaaatcga agatcgttcc aactgctttt
    17161 gaagtattta gatcgtctgc aattgattct agaaattcct ctaattgttt ttttgaatag
    17221 cctgtttgtt tagagagact aagatcatag tttaactttt ctaaaatacg agtgagtgta
    17281 ttttgaattt tagcaattgc cttactggct tcttctagag attttaagga aaaattaagt
    17341 ttagttctat aatgagcaga aaggagcaaa taacgaattg ctttgggatc gtaaccttta
    17401 gagattaaat ctctcagggt ataatagttt cctaaactct tggacatttt ttgattgtca
    17461 acgagtagat gttcgcagtg catccaaaga tttacaaaat gttcatctgg ataagctccc
    17521 atgctttgtg cgatttcatt ttcatgatgg ggaaagagta gatcaattcc accagtgtgt
    17581 atatcgattc ctgaggagta gatctttcga atcatcgcag agcattctaa atgccaaccc
    17641 ggtctaccat ttccgtattt tgtttcccag aacttttctc cttctagttt tggagatttc
    17701 cataaaacaa aatctctgac atcatctttt gtatattcat ctgcatcata gcgagcgccc
    17761 gttttcattc catcaatatc aatcttactt aactttccat aatttttgaa accggctagg
    17821 gaatagtaga tgctaccttc tttttcatag attaattttt tttcttttaa actatcaatt
    17881 aggtcgatca tttcaggaac agattctgtt gccttagggt agtgttctag tttttcaatg
    17941 tttaaagtag caaggtcttc aaaaaaaatc tgaatccatt tttttgtaaa ctcttctact
    18001 gtgacgccta actttataga ttcattgata attttatcat ctatatctgt gatattcatg
    18061 gtttggtcta attgatagcc aagtagtttt aaagatcttc ttaagacatc tgtaaaaata
    18121 taagacctga aatttccaat atgattaaaa ttataaacag taggaccaca ggaataaatt
    18181 ttaactgagt ttggattttc gggtttgaat acttcttttt tatttgtata ggaattgtat
    18241 agttttactt ctaacatgca tctctcaact ttttaccaga cttatagata gggaaagatt
    18301 ttctatttta ataattaatg taatttcgat ataacaaaaa aattaaaaga ttaccacgga
    18361 taaacaccga ggacaccaat aatgaagaaa tctaaatcca tcggtgttca gccgtgtcta
    18421 ttctgccctt cgctccaacg accaacgcct tcgcatgtgg ttaaatattt ttacatcgaa
    18481 cgcaagttaa ttagagaatt tttcctttgc tgcaagcatt gtttgcaaaa gtaaaactga
    18541 aatggtcatt ggaccaaccc cgcctggaac aggagtatag taggtagact ttgagatgga
    18601 attttttaga tcaatgtctc ctatgtttcc tggattgtag cctgcgtcta tcaaaacaac
    18661 tccctctttt aaccaatctg ctttgataaa ttctggtttt ccgacagccc caactacaat
    18721 gtctgcttgt cttacaaagt caggtaggtt ctttgtttta gaatggcaga tagtaacggt
    18781 tgcatttttt tccaaaagta gcatcgccat tggttttccc agaatcggag aacgacctac
    18841 aatgactgca tgctttccag tagggttaat tccatattct tctagaagta aaatgattcc
    18901 gtagggtgtg cagggataaa atgtcttttc tcccatggaa agttttccaa aagaaagaga
    18961 attgactcca tctacatctt ttttctctga aattctgtca aatgccaatc tttcgtcaat
    19021 ttgacttgga actggatgct gtaataaaat tccagttacc gaaatatcgg catttagcga
    19081 gtcaatgaca gaaagtagtt cttctgttgt tgtcgtttcg ggtaattcaa taagctttga
    19141 tttcatacct aatttttcac aggcattcat tttcatctta acataaactt tagaagaagg
    19201 gtcatttcct actaagatag ttgcaagggt aggaacaaag ttatgggact cttttaattt
    19261 ttgaatttca agttggatgt ttgcttttat tttctcagat aatttctttc catctaaaac
    19321 aattggtgaa ctcataaaac taaaatctca attatgcttt ttttgtctag tatacgaaat
    19381 agaaaatgaa atcaaaaaat aaagattacc accgataaac actgaggaca cagatagaat
    19441 tcaaaggaat gcagatgggt atgattttct ttgtctaacc gatgaatatt ttctattctt
    19501 ttaagggata agatgagtta agaagtaagt agaataaaat gataggaata gaaagaagag
    19561 caaaaccatc ggtgtaaatc ggttttcatc agtggtgaaa atactaacct gtttggataa
    19621 ggttatttaa tattgaaggt aggcaacaga gtgtgaagat cacactctgt atgaaaattg
    19681 gattaagcta ctgcttttgg aacgatggat atttgctttc ttttcttgtc gaaatatccg
    19741 aatgttacga ttccgtctac aagtgcaaat aaagtatggt cacgaccaat gcctacgttt
    19801 gttcctgcac gaaaaacagt tcctctttgt ctcatgagaa tatttcccgc tttaactaac
    19861 tgaccacctg atttttttag accgagtctt tgtgatcttg aatctcttcc gtttttagat
    19921 gaaccgccac ctttcttatg tgccattttt aactcctaat cagaacctga acttcctcag
    19981 ggtattgttt cgataaatta tgtattcctg ttaaaactaa ttcaaaggca agatttgtat
    20041 ccttgccggt attatttaca tcaaaattta aaaatcctgt tcggctggtt tcatgagaaa
    20101 cgttagacat aatttttaaa aataagtgta aagtttgaac taaagtagag atggctgcac
    20161 aaagaatgtt atttcctttt gatccaaaag tatctggtgc atgcccctcg ctctttaatc
    20221 ctgtataatt tccttcttgg tcttttcgaa tttctataag aatcaaagaa ctaaattagt
    20281 tacttgaaac ttttgtagat cttgtctatg accccatctt ctgtgattgt tttttcttct
    20341 tttgtatttg aaaccgtgga ttttttctcc tcttacgtct tctaggactt tcagagtaac
    20401 ttttgctcca cttacaactg gggaaccaat attcactttg ttttcttgcg ctgttaaaag
    20461 aatctgtgca tcaaattctg ttccaactgg cttatcagat ttttgcgcaa gaaaaacctg
    20521 atccttttga actttaaatt gttggttgct aagtgagata accgcgtaca taattttatc
    20581 cgttatttta atagtaaatt ccataattta tcagccttgt taggagtcaa ccgaaatgta
    20641 tcgacaacaa acctttcttg aaatttatgg tagtcatgga aattagaaat atttgtatca
    20701 tcgcacacgt tgaccatgga aagacaactt tgctcgatgg aatcttaaaa gaaacaggcg
    20761 ctattacttc aaaagaaaat agagaaagaa tgatggattc aaacgatcta gaacaagaac
    20821 gtggaatcac aattatagca aaaaacactg cagttcatta taaaggtact cgtatcaata
    20881 tcattgacac accggggcac tccgatttcg gaggagaggt ggaacgtgtg ttaaatatgg
    20941 caaattgttc ccttttgctc gtggatgcat ttgacggacc aatgccacaa actcgattcg
    21001 tattagggaa agcattgcag ttaggtcata gaccaatcct tgtagtcaac aaagtggatc
    21061 gagatggagc tagacccaat aaagtcgtag atatggtatt tgatctattt catgatttga
    21121 atgcaacaga tgagcaaatg gattttccaa ttatctttgc ttcagcaaaa ctcggttggg
    21181 cagtcaatga tatttctgaa gttccaggta aaaacctaga accactatta gatttagtat
    21241 taaaacatgt gcagctttca aaaggggatg taacagctcc tcttcaattc caagttacaa
    21301 gtctagacta caatgactat gtaggaagaa ttgcaattgg taaaatttac caaggaacga
    21361 ttcgcagagg tcaagatata acgttagtta ccatgaaaga tggtaaaact tcaaaccata
    21421 agatttctaa actgtatgga tttgaaggac taaaaaggca tgagatagaa tctgcagaag
    21481 caggagatat tgtagctcta gcgggtgttc ctgaactttt tattggagat acagtttgtg
    21541 acttaggtca accaatagca cttcctccaa ttactgtaga tgagccaact gtatctatgt
    21601 tttttatggt aaacaattct ccattcgtag gaaaggaagg aaaattagtt acgactagaa
    21661 acatacgaga aagattagat agagaattac aagtaaacgt ggcaatgcgt ttagaagaaa
    21721 cagatgacaa agatcgtttt aaaattctag gaagagggga acttcatctt tctatcctca
    21781 ttgaaaatat gagaagagaa ggatatgaat tacaagtttc tagaccagaa gtaatttata
    21841 aacaagcaga agatggaagt aagttagaac catacgaaac gttagttatg gatttaccag
    21901 accaatatac aggtcaaatc atctctgaat taaatcgtcg taaaggcgaa atgactcaca
    21961 tggacgccca tgagtccgga atcactcgtg tggaatatat cattccaact agaggactga
    22021 ttggttttag aagttatttc atttcagaaa caagaggtga gggagtttct acttctcgct
    22081 tcttacatta tggaccatat aaaggcgaaa ttcctggtag aaaaaatgga gcgttaatct
    22141 ctatggactc aggagataca actgcgtatt ctctttggaa aatccaagag agaggagttt
    22201 tatttataga tccagtcatt ccagtttatc ctggtatgat tattggagaa cattctagag
    22261 agaatgacct agaagtaaat ccttgtaagg gtaaaaaatt aactaacgtt cgtgcgagtg
    22321 gttctgatga agcaattcgt ctggttcctc caagaaaact aagcctcgaa caatcgatcg
    22381 aatttttaga tgatgatgag ttattagaag taacacctct atcactcaga ttacgtaaaa
    22441 agattctaga tgggacccag agaaaaagag ttaaataaga attgtttgtg taaagtttct
    22501 tttcgagaga agctttacac aaagtagtat ttcaaaatta aaatttaaaa acagaaagaa
    22561 tttacttttc tctatcacga atcccccttc aggaactgtg taaaagctta aaaaaatcta
    22621 accacgaaga gcacgaagat cgcgaaggtc aaaatttcca aggaaatttt aaatttctct
    22681 tcgtggcctt cgtgcacttc gtggtgaaaa tcttgttttt acacaatccc cgttccgcta
    22741 agagaatcct atcgtaagaa gacgttagct atagaataat actaatttcg gaaaaagtaa
    22801 aaaaatcaaa aaaaacaaaa aaacacccca taccactagc cgctttcaca aacataacgt
    22861 tacccacaat tatgttaaaa ctgactacca cctataaaga cgatgagcac cgatggttcg
    22921 tatagataat cggttttgcc attttacatt atttttcgat ttaatcacga ttaagcatct
    22981 aaggattcaa atattcgtct tataaaggca tttaaccgaa gtaattaaac tgatgtatga
    23041 atctatcgtt tttatcgttc ttttatcggt gtcatcggtg gtaatctgct gaaatattca
    23101 caaactccac ctgactgatt tgatttgtaa cttgaatctt gctaatactt ccatactcaa
    23161 tttttagacg aaaggaattt tctaggggca aaccgagagt atgcgcaagg atggctcgaa
    23221 tgacaccacc atgggtgaca agaattgagt ttttattttc taaaagaatt tcatttagaa
    23281 aagagataga tctagtatac agttcaaaat aggattcccc gtttgggaca tggttcttta
    23341 caaaatcaga atgccattga tcaaattctg tttttccgat ttcatcccag gatttatttt
    23401 cccaatctcc aaagttcatt tccatgagtc gtttctctaa gatgggagaa gtagaaatcg
    23461 aattggctag gactctacat ctttcgagtg ggctagaata acaggtaaag ttttcggttg
    23521 agcctaggtt agatagaacc tgtttgattt ctttttggta ggaattagcg aggctaacat
    23581 ctgtttgccc gtaacagatg ttagatggga ctgcaacttt tgtatgacga attagataaa
    23641 tttccactga gcaaataaat agacggtgat ataaaaaatg atctcggata cttgttgtgc
    23701 ggcgcctaaa caatcaccag tgtatcctcc aatatgtttt ctaaaataat aagacaaaaa
    23761 acttcgagtc agtaacaaag gaagaatcac taaaaagata aaaaactttt taaaaagaag
    23821 gagtggaagt aagccaaata gactcgctac aatgagagag ccaagaggaa gtgcagtaga
    23881 gagaggcttt gcttttcctt cagcttttac ataaaccatt gaatagagta ggctaatcga
    23941 gattaggcga ctgattgcgt gtccacttac tattgcaatc ggaacaatca tagcaggaat
    24001 ttcagataac aaaataaatt ttgttccgag tattaacact aaaccaatag ttccaaaggt
    24061 tccaattcta gagtcttgca tgatctctag aattttttcc tttgtataac ctcccccaaa
    24121 tccatcacag gtatctgcaa agccgtcttc atgaaaagcc cctgtgatta gtatagaagc
    24181 tgtcatcgaa agtagaatcg ctattttaaa aggaaataca atactagaaa acataaatgc
    24241 gatagccgtc agtgttccta caagaattcc cacaagtgga aaataacgag aggaagcatt
    24301 taatttttcc tgagaataat tgatttccgg aacaggaagc cttgtaaaaa acattaatgc
    24361 tgtaaaaaaa agttctaatt ctcttttcat gatttatttt ttactcctgc ttcttcaaaa
    24421 gaagccatat cgtttaaaaa tttcacagat gcagagacga ttggataagc aagtgctgca
    24481 ccggttccct cacccagtct cattccaaga gaaagaattg gtttgccgcc taaataattt
    24541 acaatcattt catgtgcttt ctctcctgat aaatgactga atatacaata atttattact
    24601 tctttataaa gagttttggc aaagagaagg gatgtagtca caataaatcc gtcaatgagt
    24661 ataagcattt tattctctgc tgcttgcaaa attgctccaa tcatcattgc gatttcaaaa
    24721 ccgccataag cctttagaat cgatacagcg tctgacgtat tatgcacttg actcgccttt
    24781 tttaaaagat cttttttctt ttttacatgg tcatcatttc cacttgcccc tctcccaaca
    24841 caattttcaa ttggtagatt gagtaacttg ctaattaaaa gagaagatac tgctgtattt
    24901 ccaattccca tttccccaaa accaatcaca ttgcatcctt gctcagataa atttgaaaca
    24961 atcgaggctc catgttctat tgcactttct aattcttgat ccgtcatcgc ttcttcttgc
    25021 agaatatttt tagttccaaa accaactttg gcatgaatca ttccttcagc gccttcaaaa
    25081 tcgtagttta cacccgagtc tactattttc aaatcgagtt gattttcttc acagaatgcg
    25141 ttaatggctg cacccccatg caaaaaattc atcaccattt gataggttac ttcctgtgga
    25201 taagggctca ctccttcttt tgctagtcca tggtctgcgg caaagatcac aatggtaggc
    25261 ttttttaatt taggagtagt agtgccttgg atgagtccga tttgagtggc aatgtcttct
    25321 aatctaccga gcgaccccgg tggctttgtt ttattattta tttcttctgc taattcattt
    25381 aaaatgcttt gatctggttt taatactgaa aattttttta acaatggatt atcctgcaaa
    25441 tttataacga aactatttag aagactcgtt attttgtcat tcttaaattt ttctctctca
    25501 ttcggtttga ttctaattgt tattgtttgg aaattcttcc tgagataagc ctgtcaaacc
    25561 gtttggataa aaaccataaa gaatgcattc acattcttcc catatatcat ctgcatctct
    25621 cacattttta gtattgagag gtctacattc ataggttcct gcatggtcgg ttgattcatt
    25681 gaatgataga atctttgaga gtaaggtttg ctctgatcta agcggattat ggttgttagc
    25741 tgctttcac
//
LOCUS       DAOQOS010000078        25604 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_897_length_25604_cov_5.505186, whole genome shotgun sequence.
ACCESSION   DAOQOS010000078 DAOQOS010000000
VERSION     DAOQOS010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 25604)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25604)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25604
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_897_length_25604_cov_5.505186"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(191..2206)
                     /locus_tag="PLF66_16290"
     CDS             complement(191..2206)
                     /locus_tag="PLF66_16290"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HMZ65653.1"
                     /translation="MKSPEKRSIDTKEIQNTFTLLEKASETARVGGWEYDLSLKTLSW
                     TRVTYEIHEIPFDTKLDTNLGILFYKEGYSRNRMQEVFSLLVTKGIPYDEELQIITKN
                     GNEVWVRSIGIPEFENGVIVRIYGTLQDIDRQKKDRDKLNNTLNQLEFMHKAIEDSGD
                     CFYMVNLDDHGKMIYVNEASVRHYKAPRETIYSWRVFDWDPNFTPENFPFLVEEIKNK
                     KRLHLESKHKVADGSIVPVEITVNHYLSKEGENLAYGWFSDITERIEMQNQWNEAKLK
                     AESANKAKSSFLANMSHEIRTPLNGVIGFTNILKETPLNEEQKKYLEIIDSSANSLLS
                     IVSDILDFSKIESGMLHLDIIETDLHDFFSSALDVIRFQAQSKDIQLTLSIDDKLPKI
                     GLIDPNRVKQILINLLGNSVKFTSSGSIDLNVKYQSLTESRGILTIAVKDTGIGILPE
                     EFEKLFRPFTQADTTITRKFGGTGLGLSISNMLANLMNSKIEVESEYDKGSTFYFSIE
                     IQVAEVRQEHIELLSKYKTGKERLKDLKSKILIVEDVKSNRILLKHLLQGFLPNSEIL
                     EAYDGKNAVELLETLSSNPKENYPDLIFMDVQMPILDGIEATYIIREKERKLNLNTPI
                     VALTAAALKEEEEVCYAAGMDDFLTKPIERNKLQETLVEYLILSKSN"
     gene            2660..3097
                     /locus_tag="PLF66_16295"
     CDS             2660..3097
                     /locus_tag="PLF66_16295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004784398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HMZ65654.1"
                     /translation="MKSQFDLDNSYAYMIYRTARALRRQFIRISSEQGYDLYPEQWFV
                     LVRIHNSPGCSQIDLAKDFEDRPSMTRGLRTMEQKKWIVIHKDPEDGRKNRIFLTELG
                     QELFSSMAKAVSKERNRMFLSFNENDHKRFREIIDTIFERAME"
     gene            3173..3553
                     /locus_tag="PLF66_16300"
     CDS             3173..3553
                     /locus_tag="PLF66_16300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004787317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HMZ65655.1"
                     /translation="METIEEKDLKSSLESDFLSLMGQIDKQNVSYVEEALHKDYHDSV
                     WIKGASGVFSSTKDDYLGYLSKGKIGGMDRKVEIRNLEILDQFGIIRSKLESNVMKFD
                     GGFTFFLENNQWKLIKVVMVAEKK"
     gene            complement(4032..6128)
                     /gene="mdoH"
                     /locus_tag="PLF66_16305"
     CDS             complement(4032..6128)
                     /gene="mdoH"
                     /locus_tag="PLF66_16305"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF003958.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HMZ65656.1"
                     /translation="MQQTPGSIVYTTRIVTFLALMNLIAGIGTNLLWDYFSLREMTFF
                     KWFVYGIFLILFTNLSYGTTVSIFGFWKSITGGDKYRITKNILSNKEFTLHNVPVAII
                     MPIYGEDVDSVFPRIQTMYNSIQKQKEESNFDFYILSDTQNLEKWILEETAYFELCQK
                     LNAFGRIFYRKRKMNLNKKSGNLADFCRRWGKKYRYMIVLDADSLLTGECIIQLTKLM
                     EENPSTGIIQTSPQIINAETYFQKIMQFSNLVHSNLFAVGANYWQLNSSPFWGHNAII
                     RLKPFMENCGLPSLPKLGAVGGRILSHDTIEAALIRKAGYSVWFAYDLKGSYEESPPN
                     VIDSLKRDQRWCQGNLQHFWFLFAKGLKFTSRIHILLGIFSYASSLLWFMFLVCTVFI
                     YIEDLQFYRLALGPEKWKFFWNFIYFNKALKLQIFSITILFLPRILTLLYVLIKTEYK
                     KYSQNLAIFLVNYFFEFGFSIIQAPILMYMHSKFIMLTLIGVKIEWKSQNRSANNTPS
                     FIEILNTFYILSILGILIGIYFYFQVFGLFYWMLPIWGSWAISPLFIYFISNKDSKNK
                     LLFQNSKALSDIELKNLDIEIKSKHKEITEEKNIDIDSLFLISVDPYYNSLHCFLQKT
                     THHLPEKRIKHGEYLIELLLKKGPSSLTSQDLQIILYNSIFMKKLYSKFWDSSESDLN
                     SWWNKNFVKYKMGLFI"
     gene            complement(6132..6602)
                     /locus_tag="PLF66_16310"
     CDS             complement(6132..6602)
                     /locus_tag="PLF66_16310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65657.1"
                     /translation="MNFKRLIHSQEDQDKKYFKLLALGISDFRVIIKILEKLEIQNLK
                     TNTSIQNSEIDLFVYKYLSNDTLNDIDFSTKHALALTQINNNPTELKLLINKLEKIFL
                     HKIDVSSMTPSPIHFGPLEGFFSDSLSNIDFKTLLITVGFWFGIYGLIIYYFYF"
     gene            complement(6592..8166)
                     /locus_tag="PLF66_16315"
     CDS             complement(6592..8166)
                     /locus_tag="PLF66_16315"
                     /inference="COORDINATES: protein motif:HMM:NF016252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein"
                     /protein_id="HMZ65658.1"
                     /translation="MGVVNLIMGFFAAVLIATGIKKEEPFNSEDRKPKVEILEQHKII
                     ERNFDYEELRKKVKDLYSKPYKESKKIITKNLAELNYDKYKKIRFLPEKSIWREEGSR
                     FQLQFLHPGYIYDYNVLINEVRNSKTYPIYYNPSFFDFSAIQLKDNTLNDIGFSGFKI
                     HYPINTEEHTDEFVVFQGASYFRVVSQNQFYGLSARGLATNTGMPYPEDFPIFKEFWI
                     KKPSTKDESIKVFALMDGKSAVGIYEFEFIPGKTTETLVKAEIILRKEVDRLGIAPLT
                     SMYWYGENSEANHPSAYPEVHDSDGLLILNANDEWIWRPLENPKKPTINSFNSENVKG
                     FGLLQRDREFSSYEDSEMKYHLRPGAWIQPNGNWGKGSVQLYRIPTKQDSDDNIGAFW
                     VPEEMPKVGDTFTFSYKIFWVNQNPNDKNIADVIATRVKPIPKEPDSYLYQIDFKGGK
                     LKGFDLASELEAVITSSEQGSISDISIQKVPETDKWRCSFKLTAKASSKPIELKAFLK
                     KGESVITESWTYTLEL"
     gene            8449..8913
                     /locus_tag="PLF66_16320"
     CDS             8449..8913
                     /locus_tag="PLF66_16320"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HMZ65659.1"
                     /translation="MRKNFYLVMSMAIVLTHHCATSEPAQPKIDPIAELNAELKPARV
                     SGYDLGSSTPSKSRFSEWEKNYLPVIKSVYDRMPEGYKLQIRGNADASGSNQENMEIG
                     QKRAKGIYEFLLKKGFKTDRMVVVSKGEERLMEGEADPFDEDHRRVNFRVIK"
     gene            complement(9114..9557)
                     /locus_tag="PLF66_16325"
     CDS             complement(9114..9557)
                     /locus_tag="PLF66_16325"
                     /inference="COORDINATES: protein motif:HMM:NF019238.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1569 domain-containing protein"
                     /protein_id="HMZ65660.1"
                     /translation="MVWKNTKELREYLISLEGKNITSSGNWTPSQILGHLSDSIEFVL
                     SQKKGAFEIPEWIQNTFGKLILWKFFAQGYMDKGLPNPIQKVVPDSKDIENERVRLLS
                     LMDQFENHRGTFNSHPVFGKLNKEEWNKLHLLHCENHLKYLQTKD"
     gene            complement(9699..10622)
                     /locus_tag="PLF66_16330"
     CDS             complement(9699..10622)
                     /locus_tag="PLF66_16330"
                     /inference="COORDINATES: protein motif:HMM:NF021507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HMZ65661.1"
                     /translation="MKNYFKNFLFLLLIILGLLFLSLSGFYFWLNHRSQPEAKTEEIF
                     KGVTYIRDVRKIPRPLVLHILKIDLRNKNLAFLVTPPDYSGEFVLKAQSTSEFLVKNK
                     LDIAINGDFFLPWYYNSLTDYYPHRKDPVNPNGIAISKGISYVKKTVSKFSTLFIDHQ
                     NQFSFDSKPDLKTISNAISSKPMILREGKIEKETLEKEDHFYSRIHPRTAIALDKERK
                     TLIILVADGRQPDYSMGLTGLEIIYILTEHGATDIMNLDGGGSSTLVISNGENNYLLL
                     NSPIHHNIPGIERPVANHFGIKILCKSSHCN"
     gene            complement(10619..11884)
                     /locus_tag="PLF66_16335"
     CDS             complement(10619..11884)
                     /locus_tag="PLF66_16335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65662.1"
                     /translation="MNPMKIIKYIQYFVVSILISILTCISPPKKEDSKKEEPNYLLAF
                     VTDPNGIAIYKDPSALTSKIGTIPYKQKYLEAEEYSIIPNYKNGATYAEAWHKVFKIQ
                     YANLEGSIIKNIDSSLNFYLEKQNPMFRFGVVESIDDKVYDFPFSDSKEIGKIQQFQL
                     LENSIRCVKCQEERWTQINKNGEIGYTTAKVYYYEKRIDAENAILKKIIHLNGYLKVT
                     AKSMPLLKADTFERIQNKKPKDLLEMRSEFFKVNYSTTKDKVKYYAVAGKNKEVLLTP
                     ESVGKYIEDNQFANYILSNGVYKKNECFLKTLAEKYSFSDFNFFNIEKVFLVSKKGSE
                     YYFIFPRDEILKQKNGDRIPKGAFIAKQNGRCNYITEIEDFYDYSIKKDDKIEPFQIS
                     FSIPGRGGGAESIYGFKDGEFKLLASYLK"
     gene            complement(12089..12418)
                     /locus_tag="PLF66_16340"
     CDS             complement(12089..12418)
                     /locus_tag="PLF66_16340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65663.1"
                     /translation="MTEITFIAIFLAMPIILAGGVNHSFSSLGTLITYTLLIILLNLI
                     LPILYFITRAKFLYYLLGAMDCCVGIFVFFYGLSFIHSDPFFIVGVILTLVGLTIIIF
                     PTIWKYL"
     gene            complement(12678..14030)
                     /locus_tag="PLF66_16345"
     CDS             complement(12678..14030)
                     /locus_tag="PLF66_16345"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HMZ65664.1"
                     /translation="MNIVLLKERLVKIKNKESVSLEALEYLENWIINSEPIERFQINV
                     YTVVNDNYSLNSILPIFLHGVIEEAFTLNWQMHCPHCNMVTDSFHTLREAKSISNCKM
                     CEKEYSPDFKDRVEVTFSLNKDIEDLNLPPFCTPPPSLNPLVEFVLPKGNSMESTIEL
                     EKGEYRYFCPITLAKGILFIDGEKSNDLQEIEISQLEVDYDKKEIHLQPGKIHIVGKN
                     IGAPIAGLFLHKNILMDEIPIAMIKPRLSGITLQHFPLFSKLFGTEVLSGRERLVISS
                     VTVLFTDITASTKMYETLGDVKAYNIVRDHFEILLSEIQNSQGIIVKTIGDAVMASFQ
                     KNLDSLEAIFSSMKRMKEYNRDKDISEAVFLKIGIHRGPAILVNLNDRLDYFGSTINK
                     AARIQSISKSEQISFSEEVFSDKEVRSILKANGIHRVSKSLHNLKGIEGKHSIYQVTL
                     "
     gene            14335..15063
                     /locus_tag="PLF66_16350"
     CDS             14335..15063
                     /locus_tag="PLF66_16350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65665.1"
                     /translation="MKIKSILIFLVIGLIAMNCKSMPSQGPAAKDSAVLGVKVKFNGL
                     ITDRTAARVYLVKLETPTSAKKTKDEKPVSLISTGDVIVSNWTVGNRIYYLNAQPGDY
                     ALIGAEYEVKQAKQQTSSTNVGGGVTVSTSSGGGTTIYTVFFSKDIAEKSKTTVVPGK
                     VSVIGDITVDENKDFDKADEVQKYYGERMRPGMLSVGTVMKLFTTNKLGSLKEFNNSS
                     AMKEEFLKDSKEDFNETNWTHLFQ"
     gene            complement(15309..15947)
                     /locus_tag="PLF66_16355"
     CDS             complement(15309..15947)
                     /locus_tag="PLF66_16355"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HMZ65666.1"
                     /translation="MTDSLKTNIVYGLVENDRLFSSTLAGTLRLRKETKMVYEFSSGE
                     EAVRSNTLSSINFLIVDYRLDGMDGISFLAKPEIKKLKIPKLILTGFNAEEKIFEALK
                     HGVTGYMFKEDFSSLGSILEILLAGGAFITPSIAARVCQFFEEKNESNSAQLTDRESQ
                     ILNELCNGNSPKEIAESLKIQMSTVRYHIKNIYSKLEVNNQLQLVAKVINKE"
     gene            16233..16454
                     /locus_tag="PLF66_16360"
     CDS             16233..16454
                     /locus_tag="PLF66_16360"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HMZ65667.1"
                     /translation="MEPFDLTSREAQIIDLVIQGAKNKDISDFLCISENTVKTHLQNI
                     YAKLDVCSKAQLVAFAFQNNISLPPDTAV"
     gene            17496..18368
                     /locus_tag="PLF66_16365"
     CDS             17496..18368
                     /locus_tag="PLF66_16365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65668.1"
                     /translation="MAEKSYENLYELTHKINLCLSKIEQLNKGSAILDKLILELGSLS
                     LKIKLDLKKYELSSIKTTYVIEAKSTKTTIELDTSKKDIQLNCDEEKAVSPVYLKTKY
                     PLTTWMFSWFGINRVVNSCNFGILPAGTTALIAYGDSKYINKASFDPWGNVQVEDRKR
                     QNYLFWSTTAYIRFNPFRESGMKVYLGPTVGIGYGKDNKINSDNPSLLTGFSLGLTLD
                     DTKFFALTYGIVYDPNTHMLPDYLYNRLPATFSGVNNSGTLSSFTQNQAITIPLKTVM
                     GTYHGWGFSFSYKF"
     gene            18419..19897
                     /locus_tag="PLF66_16370"
     CDS             18419..19897
                     /locus_tag="PLF66_16370"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HMZ65669.1"
                     /translation="MKKIITTILLLILVACSSGDDKSTDPVTPVPDKSTNVRAQTGTV
                     ERLQIEELTYIGPPAEKWIIGGIRGTLTNDLRRAALQKFDVLTVEDQNAALKIATDRR
                     KAGEKINLTDEVARILSIDYRCVGQVQNIGGVVRVTLKLLKAPDFKVYKSTTTDKKRT
                     ELLELQNVVIENLMHDMDEKITTEAITTMEASKTKSLLAYEFYGKGINALDDNPSQAL
                     LSFERAIEIDKNFIDALIQAGRISIYPLSDYKKGSEYLIRAKNILENQGNSNSLEYAT
                     ILRSLGQSYNHKNELSTAESYYLESKKILENLGKENTDVYVEILRDFGVQNRDLDLIF
                     RGISILEKNRKQNTLLYIDCLIGIAKLYLDEESYDNALSYYSKAQEIAENNGYKNTFQ
                     YASILSNIANTLMWKDVYNEKVLNLLNKSKAVRERLNLKNTRGYSFVLTYLGDYYRNK
                     GNNLLALKYYKEAYNLKIKLGVYEDRKRLEDQIQYLETVIQK"
     gene            19951..21201
                     /locus_tag="PLF66_16375"
     CDS             19951..21201
                     /locus_tag="PLF66_16375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65670.1"
                     /translation="MKKIIPTTSHVKVIHELPLILSNSVKTIHELSLRGGMLSLRYIR
                     SLSFLLSILLLLSCASDQGNTKLDSSFVEVTATGISLDEADTEAKLQIVEKGIGTLVD
                     GGSKTIDGESQYKVVSTYTQGFVTDFQRLSNEKVGALYKVRAKGKPNLKAIGDALEQR
                     KREVGNPKMLILMSENMFGKKTKSGSTKSEYIFQADLKKDGFQFDDKETVMKVLAREK
                     GLEVGAYGNPKAEELAQRVAVELNTDILVIGEAILKNGGEIRKGTGLLSIQADVKYKL
                     VDVNSGAILATATDTAIAPDVEPELGATEALNQVTKKLTPQIKEQIAKDWHSGSTTRI
                     SFRGIKLGEFLASNITDTIKKIRGVNGVDDRGNTGIGPTIEVKCFCGRFELLKRISRN
                     SLELDYQLTLKEEKGNLIVFDVKK"
     gene            21320..21586
                     /locus_tag="PLF66_16380"
     CDS             21320..21586
                     /locus_tag="PLF66_16380"
                     /inference="COORDINATES: protein motif:HMM:NF015630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0175 family protein"
                     /protein_id="HMZ65671.1"
                     /translation="MQNVVLEIPSDILFHIRIPENKLILELKRELAVQLYRDNLLSFA
                     NCSRMAEMTKVEFHFLLGEKKIPRHYDVDDFNQDLETIKSLDFI"
     gene            21583..22071
                     /locus_tag="PLF66_16385"
     CDS             21583..22071
                     /locus_tag="PLF66_16385"
                     /inference="COORDINATES: protein motif:HMM:NF023276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3368 domain-containing protein"
                     /protein_id="HMZ65672.1"
                     /translation="MIISNTTPLINFSSIERLDILSTLFGEIIIPPAVKNELIEKINI
                     FPKLQTVLENPNIVVLNLKDDRISGLLNIDLHLGESESIALAIENHAKILLLDEMEGR
                     RIAEYHKLKIIGTVGCLTLAKQKKIIHEVKPILTEIINKGNFWLSEKLYQNVLKTNNE
                     DF"
     gene            22615..23787
                     /locus_tag="PLF66_16390"
     CDS             22615..23787
                     /locus_tag="PLF66_16390"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HMZ65673.1"
                     /translation="MNSHGKKEELENLLHQYKDAIDQSTILSKTDKYGKITYANDEFC
                     KLSKYSREELVGKPHNIVRHPESPKSLFADLWRTIKKGKTWKGIIMNRAKDGERYVTQ
                     ATIIPIIDINGQIEEFISIRTDITELFTIKSQLQLLVEQKTEEISKTFGKLKEDLLIA
                     KKMQRSLLPSNLQTCKDLTIVAEYLPLNQVGGDIYCFDEISNSKYRIFIADASGHGVQ
                     AALVTMAIKGIYDNLKRLETNLSDILKIFNTQYIRLYGSLKTFFTCILIEIDCPNQKL
                     TYASGGHHSSVLLNGKNITLLPKTGSLLGVKDNAEYTQIERTFQKDDRLFLFTDGIFE
                     EFRNLDMFGEEKLHDIFVNNSDLPISDILQKSLSELSQFIGKSEPQDDITILGIHF"
     gene            complement(24424..24624)
                     /locus_tag="PLF66_16395"
     CDS             complement(24424..24624)
                     /locus_tag="PLF66_16395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65674.1"
                     /translation="MKIIGTRKKRTPLECIQAQNKLISELEKLRPYKKKKGIVLKFKT
                     YEELIQFDLERITKLNSPIPTI"
     gene            complement(25306..25458)
                     /locus_tag="PLF66_16400"
     CDS             complement(25306..25458)
                     /locus_tag="PLF66_16400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65675.1"
                     /translation="MEKKLRLESEAVEDLAQNYEYYESRKEGLGEEFLNDDRQKINQV
                     VNVSSI"
BASE COUNT         8683 a         3918 c         4212 g         8791 t
ORIGIN      
        1 aaaattgtaa aaaaaataaa tctgaaaatt aggataaatt aatgtccgcg taaattgtat
       61 tcccataacg cattattttc tttgagatct cttagcatgg ataatttttt atcttaattg
      121 gatatcttgt aaatcagaaa ttaagggaat tgttaaaaat gttaaaagag gtaaaaataa
      181 ttgtataatt ctaattagat tttgaaagaa ttaaatactc tactaacgtc tcttgcaatt
      241 tatttctttc aattggtttc gttaaaaaat catccattcc tgccgcataa caaacttctt
      301 cctcttcttt aagagcggca gctgttagtg ctacaatcgg tgtatttaga tttagttttc
      361 tttccttctc ccgaattata taagtagctt caattccatc taaaattggc atttgaacat
      421 ccataaaaat taaatcaggg taattttctt ttgggttaga agataacgtt tctaaaagct
      481 cgactgcatt ttttccatca taggcttcta aaatttcaga atttggtagg aagccttgca
      541 gaaggtgttt taaaaggatt cgatttgact taacgtcttc aacgattaat attttagatt
      601 ttaagtcttt caatcgttct tttccagtct tatatttaga aagtaattct atatgttctt
      661 gtcttacctc agcgacctga atttcaatcg aaaagtagaa tgtgcttcct ttgtcatatt
      721 ctgattctac ttctatcttt gagttcatga gatttgcaag catgttggaa attgacagtc
      781 cgagtccagt tcctccaaat ttacgagtga ttgtggtatc tgcttgtgta aaaggtcgaa
      841 aaagtttttc gaactcttct ggtaaaatcc caatgcctgt atctttaacg gctatcgtta
      901 aaattcctct cgattctgta agagattggt atttaacgtt taaatcgata gacccagagg
      961 atgtgaactt gacagagttt cctaacaaat taattaaaat ttgtttgact ctatttggat
     1021 caatcaagcc tattttgggt aatttatcat caatacttaa cgttagttga atatccttgc
     1081 tctgcgcttg gaaacgaatt acatccaatg cggaagaaaa aaaatcatgg agatctgttt
     1141 cgataatatc taagtgcaac atccctgact cgattttgga aaaatctaga atatcactta
     1201 ctatactcaa tagagaattt gcagatgaat ctatgatttc tagatatttc ttttgctctt
     1261 catttaatgg agtttccttt aatatgtttg taaatccgat cactccattt aaaggtgttc
     1321 tgatttcatg actcatattt gcgagaaaac tagacttagc tttattggct gattcagctt
     1381 ttaattttgc ctcattccat tgattttgca tttcaattct ttcggtaata tcggaaaacc
     1441 aaccatatgc aaggttttca ccctctttac ttaaataatg attcacagtt atttcaacgg
     1501 gcacaatgga accatctgct actttatgtt tactttctaa atgtagtctt tttttatttt
     1561 taatttcttc tactaggaag gggaaatttt ctggagtgaa attaggatcc cagtcaaaaa
     1621 ctcgccaaga atagattgtc tctcttggag ctttgtaatg ccttactgag gcttcgttaa
     1681 catatatcat tttcccatgg tcatctaaat tgaccatata aaagcaatct ccggagtctt
     1741 cgattgcttt gtgcataaat tctaattgat ttaaggtgtt atttaattta tctctatctt
     1801 ttttctgtct atcgatatct tgaagtgtgc catagattct aactataact ccattctcaa
     1861 attcaggaat cccaatagat ctaacccaaa cttcatttcc attttttgtt ataatctgaa
     1921 gttcttcatc ataaggaatc cctttggtaa ctaataatga aaaaacttct tgcattcgat
     1981 ttcgactgta tccttcttta taaaacaaaa ttccaagatt tgtgtccaat tttgtatcaa
     2041 aagggatttc gtgaatttca taggtgacac gtgtccaaga gagagttttt agagaaaggt
     2101 catactccca tcctccaact cgagcagttt cactcgcttt ttctaaaaga gtaaaagtat
     2161 tttgaatttc tttcgtatcg attgatcgtt tttcagggga tttcaaaagc tactcgtcca
     2221 atactagtaa tagtaaaaat cttataaaaa attctatcta aagtgtctgt ctgtttgtga
     2281 tttttaaata gagaagaatt ttatcataac cttttaattc ataagactga ttgtcttgaa
     2341 ttgttgttaa taaaaaaatg aataaatcca ttttataaat cacaaattat cgattccact
     2401 ttctctagag ttgaatctcc tccaagaata cctttccttt aaaaacgtca acttctattc
     2461 aacatttcgc attaacgcat attttagaaa gattaaatac ataaaaaata agatagaaat
     2521 tctatcctaa tgctttttaa aaatcagcta ataggaaagg atgttttttt cctgcaatca
     2581 ataggttcta tttttagttt gttctcattt ttttatttga caaatttttc aaaagtcata
     2641 ttgttgttat gacaacaata tgaaaagtca atttgattta gataattctt atgcttatat
     2701 gatttataga actgctagag ctttgcgtag gcaattcata cgcatttctt ccgagcaggg
     2761 ttatgatctt tatcctgagc aatggtttgt tttggttcgc attcataaca gtcctggttg
     2821 cagtcaaatt gatctggcaa aggactttga ggacagacca tctatgacta gaggacttag
     2881 gacaatggag caaaaaaaat ggattgttat ccacaaagat ccggaagatg ggcgtaagaa
     2941 tagaatattt ctaacagaat taggtcagga actttttagc tctatggcaa aagcagtttc
     3001 aaaagaaagg aatcgaatgt ttttatcttt caacgaaaat gatcataaac gttttcgtga
     3061 gataatcgat acgatttttg aaagagcgat ggagtaaaat attgccaaaa tgttgttatg
     3121 acaacaaaat gcttttttta taaagaataa attaaacgga gtgtaaaaga atatggaaac
     3181 gatagaagaa aaagatttaa aatcaagttt agaaagtgat tttctttcct tgatgggaca
     3241 gattgataag caaaatgtaa gctatgtgga agaggctttg cataaagatt atcacgatag
     3301 tgtatggatc aaaggagcct ctggagtttt tagttccact aaagacgatt atcttggtta
     3361 tctctcgaaa gggaaaattg gaggaatgga tagaaaagtg gaaattagga atctagaaat
     3421 tttagatcaa tttggaatca ttcgatctaa actagaaagt aatgtaatga agtttgatgg
     3481 tggatttact ttttttctag aaaacaacca atggaagttg attaaagttg ttatggttgc
     3541 tgaaaagaaa tagaaatcac ttaaatctac tacctaagtt gattaaacga taggtagtag
     3601 atttatttcg aattaattaa aactagaatc taattactag attttgaatt aatctctgct
     3661 cttttaaatg aataaatcga tttatttaat atctagatga attttgattt catttaaaaa
     3721 gcttgtaatt ttatcataat aaaatttaag ataagtgtat gacgtctttc aaatcctatt
     3781 aatattttct gaattggatt tgggaaaatt ttcaataaat aaaatcggac atcatttctc
     3841 aacgatcaaa tttctaaaaa tattttgaat ccatttctct ttctaataga ttcatacaat
     3901 tcttgggttg ttgtaattct ttaaagcttt taagatatct aattccaacg ttatatattt
     3961 aaaatgaatt tatcaatgca aataaaatga gcaatattat aagaagtgaa gtcttagtag
     4021 tatcactcaa attagatgaa tagtcccatt ttatatttta caaagttttt attccaccaa
     4081 gagttcaaat ctgattctga agagtcccaa aatttagaat ataatttttt cataaaaata
     4141 gagttgtata gaataatctg tagatcttgt gatgttagac tagaaggtcc tttttttaga
     4201 agaagttcaa ttaaatactc accatgtttg attcgcttct cagggagatg atgagttgtt
     4261 ttttgtaaga aacaatgcaa tgaattataa tatgggtcaa cagagattaa aaatagagaa
     4321 tcgatatcga tattcttttc ttctgtaatt tccttatgtt tagattttat ttcaatatct
     4381 aagtttttta attctatatc acttaacgct ttagagtttt ggaataataa tttatttttt
     4441 gaatctttgt ttgatataaa gtagataaag agcggtgaga ttgcccaact tccccaaata
     4501 ggaagcatcc agtaaaataa tccaaagact tggaaataaa aatagattcc aatcagaatt
     4561 ccaagtatgc ttagaatata aaaagtgttt agtatttcga taaaactagg agtattgttc
     4621 gcagatctat tttgtgattt ccattcgatt tttactccaa tcaatgtaag cataataaat
     4681 tttgaatgca tatacattaa aattggagct tggatgatac taaatccaaa ttcaaaaaaa
     4741 taattaacca aaaaaatcgc tagattctgg gaatacttct tgtattccgt ttttattaga
     4801 acataaagca acgttaatat tctaggcaag aaaagtatcg taatagaaaa aatttgaagc
     4861 tttaaagctt tattaaaata tatgaaattc caaaaaaatt tccatttttc aggtcccagt
     4921 gcaagccgat agaattgtaa atcttcaata taaataaaaa cagtgcatac caaaaacata
     4981 aaccataaca aggaagaggc ataagaaaat attccaagta gaatatgaat cctgcttgta
     5041 aactttaaac ctttagcaaa cagaaaccaa aaatgttgta agtttccctg acaccatcgt
     5101 tgatctcttt taagagagtc gattacattc ggaggtgatt cttcatagct tccttttaaa
     5161 tcataggcga accatacaga ataaccagcc tttcgtataa gagctgcctc gatagtatca
     5221 tgactgagta ttctaccgcc taccgctcct aatttcggta gggaaggaag tccacaattt
     5281 tccataaatg gctttaggcg gataatggca ttatgccccc aaaaaggaga actatttaac
     5341 tgccaataat ttgctcctac tgcaaaaaga ttgctatgaa ctagattaga aaattgcata
     5401 attttctgaa aatatgtttc tgcgtttatg atctgaggag atgtttgtat gattcctgtg
     5461 ctcggatttt cttccattaa cttggtaagt tgaatgatgc attctccagt cagtagactg
     5521 tctgcatcta aaactatcat atagcggtat tttttccccc atcgtctaca gaaatcggct
     5581 aaatttccac tttttttatt taggttcatt ttcctttttc tataaaaaat ccgaccgaat
     5641 gcatttaatt tttggcataa ttcaaagtaa gctgtttctt ccaaaatcca tttctctaaa
     5701 ttttgtgtat cgcttagaat ataaaaatca aaattggatt cttctttttg tttttggatt
     5761 gaattgtaca tcgtttgaat tcttgggaaa actgagtcaa catcttcccc ataaataggc
     5821 atgattatcg caacaggaac attgtgaaga gtgaattctt tatttgagag gatattcttt
     5881 gttatccggt atttgtcccc tccagtaatt gatttccaga atccaaaaat agaaacagtt
     5941 gttccataag aaagatttgt aaataaaatt aaaaaaatac cataaacaaa ccatttaaag
     6001 aaagtcattt ctcttagaga gaaataatcc caaagaagat tcgttccaat tccggcaatt
     6061 aaattcataa gtgctaggaa tgtaacaatt cgggttgtat aaactatcga tccaggtgtt
     6121 tgttgcataa tttaaaaata aaaataataa ataatcaaac catagatacc gaaccaaaat
     6181 ccaactgtaa tcagtagagt tttaaaatct atattggata atgaatcaga aaaaaaacct
     6241 tctaaaggtc cgaaatgaat tggacttggt gtcatactgc taacatcaat tttatgtaga
     6301 aaaatttttt ctaatttatt gattaataat tttagttcag ttggattatt attaatttgt
     6361 gttaaggcta atgcatgttt tgtgctaaaa tctatgtcgt ttaacgtatc attagataaa
     6421 tatttgtaaa cgaataaatc tatctcacta ttttgaattg atgtattcgt tttaagattc
     6481 tgaatttcaa gtttttccaa aatcttaatg attactctaa agtcagaaat gccaagagct
     6541 agtaatttaa aatatttttt atcttggtct tcttgcgaat gaattaacct tttaaagttc
     6601 aagtgtataa gtccaggatt cagtaataac tgactcacct ttttttaaaa atgctttcag
     6661 ttcaattggc ttgctacttg ctttggctgt taatttaaaa ctacatctcc atttatcagt
     6721 ttctggaact ttttgaatgg atatatctga aatgcttcct tgctctgaag atgtaatgac
     6781 tgcttctaat tcggaagcca aatcaaatcc tttcagctta ccacctttaa aatcaatttg
     6841 atagagataa gaatctggtt cttttggaat tggttttact cttgttgcaa ttacatctgc
     6901 aatgttttta tcgtttggat tttgattgac ccaaaaaatt ttataagaaa aagtaaaagt
     6961 atcaccaacc ttaggcattt cttccggaac ccagaacgca ccaatattat catcagaatc
     7021 ttgctttgtc ggaattctat aaagctgaac acttcctttt ccccaatttc catttggttg
     7081 tatccatgca cctggacgta gatggtattt catttctgaa tcttcatagc ttgaaaattc
     7141 tctatctctt tgtaaaagtc caaatccttt aacattctct gagttaaagc tattgattgt
     7201 tggcttcttt ggattttcta aaggtctcca aatccattca tcatttgcat ttagaatgag
     7261 taatccatct gaatcatgaa cttcagggta ggcggaagga tgattcgctt ctgaattttc
     7321 tccataccaa tacatacttg taagaggtgc aattccaagc cggtcaacct cctttcttag
     7381 aatgatttca gcctttacta aagtttctgt tgttttacca ggaataaatt cgaattcata
     7441 tatcccaacc gctgatttac catccattaa tgcaaagact ttaatcgact catcttttgt
     7501 agatggcttc ttaatccaaa attctttaaa tattgggaag tcttctggat acggcatacc
     7561 tgtgttagtt gcaagtcctc tcgcagataa tccgtagaat tggttttgag aaacaaccct
     7621 gaaatagctt gcaccttgga atacaacaaa ttcatctgta tgctcttcgg tattaattgg
     7681 atagtgaatc ttaaatccag agaatcctat atcgtttaac gtattatcct ttaattgaat
     7741 tgcagaaaaa tcaaagaagc taggattata ataaatcgga tacgttttag aatttcttac
     7801 ttcattgatt aaaacattgt aatcataaat atatccagga tgaaggaatt gaagttgaaa
     7861 tcgagatcct tcctctctcc aaatagattt ttcaggaaga aatctaatct ttttatactt
     7921 atcataattt aattcggcta aatttttagt aatgattttt ttagattctt tgtatggttt
     7981 agagtataaa tccttaacct ttttcctaag ctcctcatag tcaaaatttc tctcaatgat
     8041 tttgtgttgt tccagaattt cgactttggg ttttctatct tccgaattga atggttcttc
     8101 ttttttgatt ccagtcgcta ttaagactgc tgcaaaaaat cccatgatta aatttactac
     8161 tcccatactt accacctata tatattaatt aaaatagatt gaatgaggca ccgcccattt
     8221 tctttatcgt tttatctctg acaacttaaa aaaatatttg agatacttaa gtaaaaatta
     8281 cggggtaatt atgattatcc tgtggattat acttttttta tccaatttat attcacagta
     8341 aaatcaaaaa aaaataaaaa aatatttttt aattttaaga aaattatttt tttcgtgtct
     8401 attcattttg aaaataagag atcaaattta aatttggagt aaaagtaaat gagaaaaaat
     8461 ttttatttag taatgagtat ggctattgtc ctaacgcatc attgtgctac ttctgaacct
     8521 gctcaaccaa agatcgatcc aattgccgaa ttgaatgcag aactaaaacc tgctagagtt
     8581 tcgggttatg atttagggag ttcaactccg tctaaatcta gattttctga atgggaaaaa
     8641 aattatttac cagtcattaa atctgtttat gatagaatgc cagaagggta taaactacag
     8701 attaggggaa atgctgatgc ctctggttca aatcaagaga atatggaaat cggacagaaa
     8761 cgggcaaaag gtatttatga atttcttctt aaaaagggat ttaaaacaga tagaatggta
     8821 gtcgtttcaa aaggggaaga acgattgatg gagggggaag ccgatccatt tgatgaagat
     8881 cacagaaggg ttaatttcag agtaattaag taagaagcga atcttttatt tattgagttt
     8941 tatcagactg tgtaaaagta tatttcgaat tatgattttt tttcgatttg cttttacata
     9001 gtttttttta tctaatttaa tctttgtgct agtatttgat agaaaggatt ctcatcgcac
     9061 gaataaaaaa gattatttag gtattctatt ctatagaatt catatatttt tattcaatct
     9121 ttagtttgaa gatactttaa atgattctcg caatggagta ggtggagctt gttccattcc
     9181 tctttattta gttttccaaa aacaggatga gaattaaaag ttccccgatg attttcaaat
     9241 tgatccataa gtgaaagtag tcgaactcgt tcattttcaa tgtccttaga atcgggaacc
     9301 actttctgga tagggttagg aagtccttta tccatataac cttgcgcaaa gaatttccaa
     9361 aggattaatt ttccaaaggt attttgaatc cattctggaa tttcaaaggc tccttttttc
     9421 tgggataaaa caaattctat cgaatctgat aggtgaccta gaatttgaga aggtgtccag
     9481 ttgccggatg acgttatatt ttttccttct agagaaatta aatattctct tagctctttt
     9541 gtgtttttcc agaccattca tttttaccca tatttttaat ctaaggttga atcctttgtt
     9601 agaataaata tagttgtaac agtttcagaa gcaagaatta attcttctaa tttgatttag
     9661 aagaacgctt tctattttat gtctgactat aatccgaatc aattgcaatg ggatgatttg
     9721 cataaaatct tgatcccaaa atgatttgca actggtcttt ctattcctgg tatattatga
     9781 tgaatcggag aatttaagag taaataatta ttctctccat tcgaaattac aagggtagaa
     9841 gatccacctc catctaggtt cattatatcc gtagcaccat gctctgtcag tatataaata
     9901 atttctaacc ctgtaagtcc cattgaatag tctggttgcc taccgtccgc tacgaggata
     9961 atcaatgttt tcctttcttt atcaagtgca attgcagttc taggatgaat tctagaataa
    10021 aaatgatctt ctttttctaa agtttccttt tcgatttttc cctctcgcag aatcataggt
    10081 ttacttgaaa ttgcattgga aattgttttt aaatcaggct tcgaatcaaa actaaattga
    10141 ttttgatggt caataaatag tgtggaaaat ttactaacag tttttttgac ataggagatt
    10201 ccttttgaaa tcgcaattcc atttggattt actggatcct ttctatgtgg ataataatcg
    10261 gtaagagaat tataatacca gggtagaaag aaatcaccgt taatagcaat atctaattta
    10321 ttttttacta agaattcaga tgtgctttgc gcttttaaaa caaattcacc tgaatagtca
    10381 ggcggcgtga ctagaaatgc aagattttta tttctaagat caatttttaa aatatgtaag
    10441 accaaaggtc taggaatttt tctaacatct cgaatgtatg taacgccttt gaatatttcc
    10501 tctgttttag cttccggttg tgatctatga ttgagccaaa aataaaaccc cgatagactt
    10561 aagaatagaa gcccgagtat tatgagtaag agaaataaaa aatttttgaa ataatttttc
    10621 atttcaaata agaagctaga agcttaaact ccccatcctt aaagccatag atagattccg
    10681 cgccaccacc tcgaccaggg atagaaaaag atatttgaaa tggttctatc ttatcatctt
    10741 tcttaatact ataatcatag aaatcttcaa tctcagttat ataattacaa cgaccgtttt
    10801 gttttgctat aaaagcacct ttgggaattc tatctccatt cttttgtttc agaatttcat
    10861 ctcggggaaa aataaaataa tactcactcc ctttttttga aactagaaat actttttcta
    10921 tattgaaaaa attaaagtcc gagaaggaat atttttctgc caatgttttt aaaaaacact
    10981 catttttctt gtaaactcca ttagaaagga tataatttgc aaattgattg tcttcaatgt
    11041 attttccgac gctttctgga gttaatagaa cttccttatt tttaccggca actgcatagt
    11101 attttacttt atcttttgtt gttgagtaat ttactttaaa aaattcactt cgcatttcta
    11161 ataaatcttt tggcttttta ttttgaatcc tttcaaaagt atctgctttg aggagaggca
    11221 tggactttgc ggttactttt aagtatccgt ttaaatgaat aattttcttt agtattgcat
    11281 tttctgcgtc gattctcttt tcataatagt aaacctttgc agtcgtatat ccaatttctc
    11341 cattcttatt tatttgcgtc caacgttctt cttggcattt aacgcatctt atagagtttt
    11401 ctaacaactg gaattgctgt atctttccaa tttcttttga atcagaaaaa gggaagtcat
    11461 agaccttatc atcaatcgat tctacaactc caaacctaaa cattggattt tgtttttcta
    11521 aataaaaatt taaactagaa tctatatttt taattataga accttctaaa tttgcatatt
    11581 gaattttaaa aaccttatgc catgcttcag catacgttgc tccattctta tagttgggaa
    11641 ttattgaata ttcttcagct tctaaatatt tctgcttata gggaattgtg ccaattttag
    11701 aagtcaaagc acttggatct ttataaatag cgatgccatt tggatctgtt acaaaagcta
    11761 acaaataatt aggctcttct tttttagaat cttctttttt cggtggagaa atacatgtta
    11821 gaatagatat taaaattgaa acaacgaagt attggatgta ttttataatt ttcattggat
    11881 tcatcgaaga tttcactgat ctagaaatta atttttggat agatacgaag ttaggtcaac
    11941 tttttatttg ctatccttta cactgaacca ttaaataaat ccttacacaa aattgatagt
    12001 ttgtggagta aaagaatatt ttttaaataa aaaaatacca agcaagcaat aagcgcgaag
    12061 aaatgacagg tgagtgatta tctatgaatt acaaatactt ccatatggta ggaaaaataa
    12121 taattgtaag accgaccaaa gtaaggatta cgcctactat aaaaaagggg tcactatgaa
    12181 tgaacgatag tccataaaag aaaacaaata tacctacaca gcaatccatt gcacctaaaa
    12241 gataatataa aaattttgct cgagtgatga aataaagtat tggtaaaatt aaatttaata
    12301 aaatgattaa aagcgtatac gtgattagag ttcctaacga actgaaagaa tgattcactc
    12361 ctccagccag tatgataggc attgcaagga aaatggcaat gaaagtaatt tcggtcatat
    12421 tttctctctg tttttagcta aattataatt ctagaattaa cagatcaatt ggcgatattt
    12481 ctcatagcct actaaaatta tgaaatagat tggagaaatt ttttatccga aaaaaaccgg
    12541 ctaatggata acaaatattt cgaggatttt cgttagagag tatagaccaa gtaagattcg
    12601 ctatctatta caggcgtttc caaaatttat ttcatctata tggttgagtg ctcaatagtt
    12661 ggaaaaaagg acaacatcta gagtgtaact tgatagatag aatgttttcc ttcgattcct
    12721 tttagattat gcaatgattt tgagactcga tgaattccat ttgctttcag gattgaacga
    12781 acttctttat cagaaaaaac ttcttctgaa aacgaaattt gttcgctttt agagatggat
    12841 tgaattctag ccgccttgtt gatggttgat ccaaagtagt ctagtctgtc attcagattt
    12901 actaagatgg caggtcctct atgaatccct atttttaaga agactgcttc tgaaatatct
    12961 ttgtctctat tgtattcttt catccgtttc atggaagaaa agattgcttc caaagaatct
    13021 agattttttt gaaaagaagc catcactgca tccccgattg tttttacaat aattccttgg
    13081 ctgttctgaa tttcggaaag tagtatttca aaatgatcgc ggacaatatt atacgctttt
    13141 acatcaccta acgtttcata catcttagtc gatgcagtaa tatcagtaaa aagaacagtc
    13201 acagaggaaa taaccagacg ttctcttcca cttaatactt ctgttccaaa cagcttcgag
    13261 aacaaaggaa agtgttgtag tgtaatacca cttagcctcg gttttatcat tgcaattggg
    13321 atttcatcca ttaaaatatt tttatgtaaa aataaaccag caatgggagc cccgatattt
    13381 tttcccacga tatgaatttt gcctggttgt aaatgaattt cttttttatc ataatcgact
    13441 tctaactggc tgatttcaat ttcttgcaaa tcattcgatt tttctccatc gataaataag
    13501 attccttttg caagagtgat cggacagaaa taacgatatt ctcccttttc caattcgatt
    13561 gtgctctcca tagaatttcc ttttggtaat acaaattcta ctagaggatt tagactaggt
    13621 ggtggtgtgc aaaaaggtgg taggtttaag tcttctatat ctttattcaa agaaaacgtt
    13681 acttccactc tatctttaaa atctggggaa tattcttttt cacacatttt acaatttgaa
    13741 atggactttg cttctcttaa tgtatgaaag gagtctgtca ccatgttaca atgagggcag
    13801 tgcatttgcc aatttaatgt gaatgcttct tctattactc catgcaagaa aataggtaaa
    13861 attgaattta gcgaataatt gtcatttacc actgtatata cattgatttg aaaacgctca
    13921 atcggctctg aatttatgat ccaattctct aaatattcca atgcttctag agaaactgat
    13981 tctttgtttt taattttaac tagtctttct tttaataaaa caatattcat gtatatttct
    14041 cccgttgcat tcatctatca cgatacaaat acaatctcta tatgttttct tgaagtcatt
    14101 atttttattt attttgtgat tagaaaatgt aagatattct tcaactaact tttagatata
    14161 ccataaatgg gtcttttatt attcttgact gaattataaa aggtttaaat atttttagtt
    14221 tcttcattcc tcgattcatt tcttttgttc actatttaaa attaaatcca atgtcatgaa
    14281 aactagcttg cccaataaaa tctgggaaat agattgaggc atttcggagg gaaaatgaag
    14341 ataaagagta ttttaatttt tttagtgatt gggcttattg ccatgaattg caaatcaatg
    14401 ccaagtcagg gacctgcagc taaagacagt gctgttttag gagtaaaagt aaaatttaat
    14461 ggtttgataa ccgataggac agcagcaaga gtttatttag tgaaattaga aactccaacg
    14521 agtgctaaaa aaactaaaga tgaaaaacca gtttcattga ttagcactgg tgatgtgatc
    14581 gtttcgaatt ggactgttgg caatcgaatt tattatctaa atgcacaacc aggcgactat
    14641 gctttaatag gggctgagta tgaagtgaag caagcaaaac aacaaacgag ttcaactaac
    14701 gttggtggtg gagttacagt gagcacatct tccggaggag gaactaccat ttatactgta
    14761 tttttttcaa aagatattgc ggaaaaaagt aaaactactg ttgttcctgg aaaagtatcg
    14821 gtgattggag atataactgt tgatgaaaac aaagacttcg ataaggcaga cgaagtgcaa
    14881 aaatactatg gtgaaagaat gcgacctggt atgctaagtg ttggaaccgt aatgaaatta
    14941 tttacaacga ataaattagg ttctttaaaa gaatttaata atagtagtgc aatgaaagaa
    15001 gagtttttaa aagattctaa agaagatttt aatgaaacaa actggactca tttatttcaa
    15061 taaaacattt atatagacta acggtttatt acattgaatc tctagtagga tttataaaat
    15121 tttactagag attgattttt acttaaacgg gagagtcaga ttcagttctt agaaaatcta
    15181 gattctttca actttaaaag attttaaaat tataaaactc gaaacaaaat ataaaaatat
    15241 cattcgatca aaaaattgta gagtagatcc attgaataaa tggaaatgct ctagcattta
    15301 gaatggtttt attctttatt gatgactttg gcaacaagtt gtaattgatt attcacttcc
    15361 aatttgctat aaatattttt gatatggtat cttacagtag acatttgtat cttcagggat
    15421 tctgcaattt cctttggaga atttccattg caaagttcgt ttagtatctg actttcccta
    15481 tcggttagtt gtgcagaatt cgattcgttt ttctcttcaa aaaattgaca tactcttgca
    15541 gcgattgagg gagtaataaa tgctcctcct gctaaaagaa tttcaaggat tgaacccaga
    15601 gaagaaaaat cttctttgaa catatatcca gttacgccat gctttaatgc ctcgaaaatt
    15661 ttttcttctg cattgaaacc tgtcagaatt aatttaggga tttttagttt tttaatttct
    15721 ggttttgcta aaaaactaat tccatccatt ccatcgagtc gatagtcaac gatcaaaaaa
    15781 ttgatacttg aaagagtgtt tgatcgaact gcttcttccc cagaggaaaa ttcgtaaacc
    15841 atttttgtct cttttctgag tcgcaaggtt ccggctaacg tagaactgaa gagtctatca
    15901 ttctcaacga gtccataaac tatatttgtt ttgagtgaat cggtcacgtt taatattgat
    15961 ttgcccaagc attcctaatt tcaacagaaa aactactaaa attaggtatt ttccattaaa
    16021 aaaaatattt ttttcatagg atgactatca agatggggga tggactatca tgccattcgg
    16081 atctataatc actcaaaaga attactatat cggtaatgaa atcctattga actatctata
    16141 catacaatag aaaatgattg gatttactat aaataacaag gaaaaataaa tgagaattta
    16201 tagacaagta aattctttaa aaggtattta gaatggaacc ctttgactta acttctcgtg
    16261 aagcacaaat tattgacttg gttattcaag gtgctaaaaa taaagatatt tctgattttc
    16321 tctgcatttc agaaaacacc gtcaaaactc atttgcaaaa tatttatgca aagcttgacg
    16381 tctgctccaa ggcacagcta gttgcattcg cttttcaaaa taatatcagt cttccacctg
    16441 atacggcagt atgatttttt aaccaccgat acacaccgat gggcaccgat aaaagatacg
    16501 attttaaacg atggatttgg gaaatagatt tcagcccata ctatgaggaa aggttactcc
    16561 ttataccaaa cgtcaaaaga aaaatcccat ttcaaaaata aaaaggtggg tttggtctaa
    16621 acacttctta gtgttcccga acatcagtat gttcggtata ccaaatgcta ttcagttaaa
    16681 atccttctgg gaaattactt ttgtcatttg gtatattagc cgattcgatc tttttgaatt
    16741 aagttctttt cactatgagg tgaacgaaga ccctactgtt cagctattta ctttgttttt
    16801 ctttctaccc tgttctctgt ggaaaaattt taggatgctt ctgaaataaa taatccaaag
    16861 tgtttaaact tttattggct atcattctct tagtcgtatt tcctacaaat ccccagtgtt
    16921 attaaataaa aagataattg caaattttat gaacgctatg cacctataga cactaatgaa
    16981 ggatacagta gtattttaat taaattctat tagaaagact ttctatttaa gtgaactatc
    17041 gatagtcgta taacaacgct tatccgaagc gatagttgaa aaaatcaccc ctttgggtga
    17101 ttgacaaaat atataattta aaatatgcgc ccgaactatc gtattatgga aaatatttat
    17161 ctatcaaact ttttttcttg taaatttttt tgttccaatc ttagttggtt tttctaagag
    17221 agacagtgat atgtttatag cgaaacagcg aaacagcgaa acagcgaaac agcgaaaatg
    17281 gtaagcttta ccttaaacta ttcttaatta atattttaat ttttgggtat cctgctttac
    17341 atgcgaaaga tgtaaaagag actaagcaag ctagtgatat caatgaatca tcctgttatt
    17401 ttgacaatgg ccaaaattct gagactttag taattttcga aaatgacagt aatcagatca
    17461 ttaaagattt taattctaaa tactatgctt acaaagtggc agaaaaatcg tatgaaaacc
    17521 tttatgagct aactcataag attaatttat gtctgagtaa aatcgaacaa ctcaataaag
    17581 gtagtgcaat tttagataaa ttaattctgg aactaggaag tttatctctt aagattaagc
    17641 tagatttaaa aaaatatgaa ttatcatcaa tcaaaacaac gtacgtaata gaagcaaagt
    17701 ctacgaaaac aacaattgag ttggatactt cgaaaaaaga tattcaattg aattgtgacg
    17761 aagaaaaagc tgtttcgcca gtttatttaa aaacaaagta tccactgaca acttggatgt
    17821 ttagttggtt tggtataaac agagttgtaa actcttgcaa ctttggaatt cttccggctg
    17881 gaacaactgc tctcattgca tacggggata gtaaatatat aaataaggca agttttgatc
    17941 cttggggtaa cgttcaagta gaagacagga agcggcaaaa ttatcttttc tggtctacga
    18001 cagcttatat tagatttaac ccgtttcgcg aatctggaat gaaagtatat ctgggaccta
    18061 ctgttggtat aggttatgga aaggataata aaataaattc cgataaccct tctcttttaa
    18121 ctggattttc tttaggttta actttggacg atactaaatt ttttgctctt acttatggta
    18181 ttgtttatga tccaaatact catatgttgc cagattatct atataaccgt ttaccagcaa
    18241 ctttttcagg agtaaacaat agtggcacat tatcctcgtt tacacagaat caggcaatta
    18301 caattccttt aaaaacagtt atgggaactt atcatggctg gggatttagt ttttcgtata
    18361 agttttaaaa ataataatat ataaaatagt agggaacaca aggaaccaaa taaaacccat
    18421 gaaaaaaata ataactacaa tcttattact aattctagtt gcgtgttcta gtggagacga
    18481 taaatcgact gatcctgtta cccccgtgcc ggacaagtct acaaatgtta gagctcagac
    18541 tggaacagtg gagcggttac aaattgaaga gttgacttat atcggaccgc cagcagagaa
    18601 atggatcata ggtggaatta ggggaacttt gacaaatgac ttacgaagag ctgcactgca
    18661 aaaatttgat gtattaacag ttgaggatca aaatgctgct ttaaaaattg ctacggacag
    18721 aaggaaagca ggggaaaaaa taaatttaac ggatgaagtg gcaagaattt taagcataga
    18781 ctatcgatgt gttgggcaag ttcagaatat tggcggggta gtaagggtga ctcttaaatt
    18841 gctaaaggct cctgacttca aagtttataa gtctaccact acggataaaa aaagaacaga
    18901 acttttggaa ctgcagaatg ttgtaattga aaatttaatg catgatatgg atgaaaaaat
    18961 aacaactgaa gccataacta ctatggaggc atctaaaact aaaagtcttt tagcttatga
    19021 gttctatgga aagggaatta atgctttgga cgataaccca tctcaagcgt tactttcttt
    19081 tgaaagagcc attgaaattg ataagaattt tattgatgca ttaatacaag caggtagaat
    19141 ttcaatctat cctctgagtg attataaaaa gggctctgaa tatttaattc gggcaaagaa
    19201 tatacttgag aatcaaggca attcaaactc actagaatat gcaacaatct tgcgtagttt
    19261 aggacaatct tacaatcata aaaacgaatt atccaccgca gaaagttatt atttagaatc
    19321 gaaaaagatt ctggaaaatt taggaaagga aaatactgac gtttatgtag aaatactacg
    19381 tgatttcgga gtgcaaaatc gagatctaga tttaattttt cgagggattt ctattttaga
    19441 gaaaaataga aaacagaata ccttacttta tatcgattgt ctaattggaa tagctaaact
    19501 atacctagat gaagaatctt atgacaatgc tctttcttat tatagtaaag cacaagaaat
    19561 agccgagaat aatggctata aaaatacttt tcaatacgca tccatccttt ccaatatcgc
    19621 taatacttta atgtggaaag atgtttacaa tgagaaggta ttgaatttat tgaataagtc
    19681 aaaagctgtt agagaaaggt taaatttgaa aaacaccaga ggctatagtt ttgtattgac
    19741 ttatttggga gattactatc gaaacaaggg taataattta ttagcgctaa aatactacaa
    19801 agaagcttat aatttaaaga taaaactggg agtttatgaa gacagaaaac gtctagaaga
    19861 ccagattcaa tatctagaaa ccgtcattca aaagtaataa tgtttaacta tggtaaataa
    19921 aaatactttt aaattctaaa ggaactgatc ttgaaaaaaa tcatacctac aacgtcacac
    19981 gtaaaggtaa ttcacgaatt gcctctaatc ctatcgaact ccgtaaagac aattcatgaa
    20041 ttgtctctac ggggtggtat gttgtctcta cggtatatac ggtctctatc ttttctccta
    20101 tcgattcttc tcctcctatc ctgtgcatcg gatcagggga atacaaaatt ggacagtagc
    20161 tttgtggagg tgactgcaac gggaatttct ttagacgaag cagatacaga agcaaagcta
    20221 cagattgtag aaaaaggaat tggaacattg gtagatggtg gaagcaagac gattgatgga
    20281 gaatcccaat acaaagttgt ttccacttat acacaaggat tcgtgacaga ctttcaaaga
    20341 ctttcgaatg aaaaggtagg tgctctctat aaagtgagag caaagggaaa gccgaattta
    20401 aaagcgatag gcgatgcact tgagcagaga aagagagaag ttggaaatcc gaaaatgctg
    20461 attttgatga gcgagaatat gtttggtaaa aaaacaaaat cgggttctac taaaagtgaa
    20521 tacatatttc aagcagatct aaaaaaagat ggattccagt ttgatgacaa agaaacagtg
    20581 atgaaagttc ttgcccgcga aaaaggttta gaagtgggag cttatggaaa tccaaaagca
    20641 gaagagctag cgcaaagagt cgctgttgaa ttaaacacag atattttagt aataggagaa
    20701 gccattttaa aaaatggagg agagattcga aaaggaacag gactcctttc gattcaagca
    20761 gacgttaagt ataagcttgt cgatgtgaat tcaggtgcga ttttggcaac cgctactgat
    20821 acagctattg ctcctgatgt agagccagag ctaggtgcaa cagaagcgtt aaaccaagtt
    20881 actaaaaaat taactccgca aatcaaagaa cagattgcaa aggattggca ttcaggaagc
    20941 acaactcgta tttcatttcg tggaatcaag ctaggagaat ttctcgcaag caatataact
    21001 gacacaatca aaaagattcg aggagtgaat ggcgtagacg atagaggaaa tacaggcatc
    21061 ggaccaacca ttgaagtcaa atgcttctgt ggacgcttcg aactcctaaa acgcatttct
    21121 cgaaacagtt tagaactaga ctaccagctt actctaaaag aagagaaggg aaatttgatt
    21181 gtgtttgatg tgaagaagta gtctggacag ggattttgtt ttcctagacc agtcctttag
    21241 ggcttggcgg atgttaattt ccaaaaacga atcaaggatt tttaattgac agagatagga
    21301 atgaagcaag aataaaagaa tgcaaaatgt cgttttagaa attccttctg atatactttt
    21361 tcatataaga attccagaaa ataaattgat tctagaatta aagagagaat tggctgttca
    21421 gctttataga gataatcttt tatcttttgc aaattgctct cgtatggctg aaatgactaa
    21481 ggtggaattt cactttcttc ttggtgaaaa gaaaattccg cgtcattatg acgttgacga
    21541 ttttaatcaa gatttagaaa caataaagag tttggatttc atttgattat ttcaaatact
    21601 actcctctca taaacttctc ttctatagaa cgactagata ttctttctac tctatttggt
    21661 gaaataatta ttcctcctgc tgtaaaaaat gaattaattg aaaagataaa tatttttcca
    21721 aaacttcaaa cagtattaga aaatccgaat atagtagtgt taaatcttaa agatgataga
    21781 atatctggtt tactaaatat agatttacac ttgggagaat ctgaatcaat tgccctcgct
    21841 atagaaaatc atgcaaagat tttactgcta gatgaaatgg aaggaagaag aattgcagaa
    21901 tatcacaaat tgaagattat aggaacggtt ggctgtttaa cattggcaaa gcagaaaaaa
    21961 attatccatg aagtcaagcc catccttaca gaaattataa ataagggaaa tttctggctg
    22021 agtgaaaaat tataccaaaa tgttttaaaa acaaataacg aagatttcta attaaaaaag
    22081 gaatacaaac agatatattg atctattcag aaaaagaagt aaatattaag aaaactcttc
    22141 ttagttatat catcataaat gcagatagag tttgttcgag aaagaatatc tgtttgatat
    22201 tcagataaaa tattagtatc gcagactatc ttatccaaag acttttcttg aattctgata
    22261 catttaattt tttatttttc cataatccgt aaaatctttt tggctctcgt tccattaatg
    22321 ccaccgcaac cgatcaacta tagcatctga tgtttataga gagtttggag caatagaggc
    22381 attgaaccaa atcctaaaaa aatcacccct ttgggtgatt gactgaattt agaaatgaag
    22441 atacactgta agcattgata ggagtagttt cccaaaatat acccatccta aacagtcaaa
    22501 ctgtttagga tgggctttgc atcagtatca gaatagctgg aaatagcttt atctatttac
    22561 tagatgaatt ctacttatcg catatcttta caattgggaa acgaatcaag ctagatgaat
    22621 agtcatggga agaaagaaga attagagaat ttacttcatc aatataaaga cgcaatcgac
    22681 caaagcacta tactttctaa aacagataag tatggaaaaa ttacctatgc aaatgacgaa
    22741 ttctgtaaac tatctaaata tagtcgagag gaattagtcg gcaaaccgca taatatagtt
    22801 cgtcatccag aatccccaaa atcactattc gcagatttat ggagaacaat aaaaaaagga
    22861 aaaacttgga aaggcataat catgaaccga gcaaaggatg gagagcgtta tgttacacag
    22921 gctacaatca ttcctatcat agatatcaat ggccaaatag aggaatttat aagtatacga
    22981 actgatataa cagaattatt tacaataaag agtcagcttc aattgcttgt tgagcaaaaa
    23041 acagaagaaa tcagtaaaac atttggaaaa ttaaaagaag acttattgat tgcaaaaaaa
    23101 atgcagagaa gtcttttgcc cagcaattta caaacatgta aagaccttac tatcgttgct
    23161 gaatatctac cactcaatca agttggcggt gatatatatt gctttgatga aatatcaaat
    23221 agtaaatata gaatttttat tgcagatgct tctggtcatg gagtgcaggc cgctcttgtt
    23281 acgatggcaa ttaaaggaat ttatgataat ctaaaaagat tagagactaa tctttctgat
    23341 atactaaaaa tatttaacac tcagtatata cgtctctatg gatctcttaa aacttttttt
    23401 acctgtattt taatcgaaat tgattgtcct aatcaaaaac taacatatgc ttctggtggt
    23461 catcattctt ctgttttatt gaatggtaaa aacataactc ttttaccaaa aactggatct
    23521 cttttagggg ttaaggataa tgcggagtat acgcaaatag aaagaacttt tcaaaaagac
    23581 gatagactat ttttatttac ggatggaatt tttgaagaat ttagaaattt ggatatgttt
    23641 ggggaagaaa aattacatga catttttgtg aataatagcg atttgccaat ctctgacatt
    23701 ttgcaaaaat ctctttcaga gttaagtcaa tttattggga agagtgaacc acaagatgat
    23761 attacaatat tagggattca tttttaggat tctaccttcg ctctttaaaa taaaagtaga
    23821 actcctccca tttggtaaat atggagtaac ttctgttttc atacaatgtt tcaaattaaa
    23881 cgatagccgt aggaatcaga ctgtctgatt cttacggatt tttttttacg acctagagca
    23941 aaaagattac gaagtgtagg tatttacttt atttattttc gatgcggcta atattgtttt
    24001 tcctataatt atcttagtcg caatttatgt aagttaattg taaatattat aaacgcgatg
    24061 gacatataaa tactaattaa ggatacagta gtattttaat tagattcttt tagaaatatt
    24121 ttctacttta gtgaactatc gataggtgta taacaatgat tagtcaaaac gatagtagta
    24181 aaaatcacac attcgggtga ttgataaaat taatacaata agatagtcga aaaaactaac
    24241 gtattgtaaa aaatattact ttttaaaaat aatcttgtcg ttttttttgt tccaatttta
    24301 gttggttcct tgataaagaa ggagtttctt ctgcaggagg ttgacttgat tctaaaatgc
    24361 agtttctatg agtcttttag tgaaaatcct gtaacggaaa gtccttaata aataaacttt
    24421 ttattagatg gtcggtattg gtgaatttag ttttgttatc ctttctaaat caaattgtat
    24481 taattcctca taggttttaa attttagaac tatacctttt ttctttttat agggacgtag
    24541 tttttctaat tcagaaataa gtttattctg cgcttgaata cattctaatg gagttctttt
    24601 ttttcttgtg ccaatgattt tcatttgtta taaattttca gattctttcg aattttacaa
    24661 gtagaaaaag aggcttttga atcaatgact gtttgttttc ttcgcatgaa acatatacca
    24721 aacttccctt aaagatccct taaacttagg gtaaagatcc ctatttgatt cgcaaaggga
    24781 agtttacctt aactttaccc taagattagg atatctttgg tgtgaaggga atgcggtgat
    24841 ttcgaaagaa attcatcctt tattttctat cagatcagaa tttcgtttat tagaaataga
    24901 aatctagata ctgcttttac ttttatgcgg caaatgagga gtaaccgcat aaaagaagta
    24961 aaaaattcat aagtaaaacc aaactaaatg aaaaatattg taaagaggga tattgaataa
    25021 atggattgtt ttatctatat ccttgaaatc ttctatattg taagtaaata aatctgcctg
    25081 tatgatctta acggttgcta atagaattgc gtctggaagc tttttcccat tcagatctca
    25141 attttctttg ccatttaaca ggatctgaaa ttctagagaa tactatatac catctaggct
    25201 ctcagaattt ttcataaatt ggcattgatt tgattttgaa ttctattcct tccattgatc
    25261 ggtgttaatc gtcttcatcg gtggtaatct tttatttttt tattgttata tcgaactgac
    25321 gttaactact tggtttatct tttgcctatc gtcatttaaa aattcttctc ctaagccttc
    25381 ttttctactt tcatagtatt cataattttg tgctaaatcc tcaacagctt ctgattcaag
    25441 tcttaatttt ttttccaaat cggttctcca aaatttggat agattcttcc caagtataac
    25501 ctggatgtgg ttccttttgc attcttaaaa gacgagagtc tatttcttct ttatagaagc
    25561 tatctgtttc tggtgaaaga atttcttctg aatccaatgg ctct
//
LOCUS       DAOQOS010000079        25374 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_916_length_25374_cov_6.549666, whole genome shotgun sequence.
ACCESSION   DAOQOS010000079 DAOQOS010000000
VERSION     DAOQOS010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 25374)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25374)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25374
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_916_length_25374_cov_6.549666"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(652..801)
                     /locus_tag="PLF66_16405"
                     /pseudo
     CDS             complement(652..801)
                     /locus_tag="PLF66_16405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002105553.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
     gene            1017..1334
                     /locus_tag="PLF66_16410"
     CDS             1017..1334
                     /locus_tag="PLF66_16410"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HMZ65676.1"
                     /translation="MISVNIEPNQISNIRDVVKGYFQTKPVNKVYLFGSYARKEAGKN
                     SDIDLLLDLIYEPGIAFIFGMMKQELELLLHKKVDLITTNSISKYIAENIEKEKILIY
                     EKI"
     gene            1321..1671
                     /locus_tag="PLF66_16415"
     CDS             1321..1671
                     /locus_tag="PLF66_16415"
                     /inference="COORDINATES: protein motif:HMM:NF014043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HMZ65677.1"
                     /translation="MKKFNSDKIWLESILENIHSLESFSAGMSYEIFDRDLKTKFACE
                     KCIQNIAEASNNLSLEFKQENKNFPWSQMKAMRNIITHEYFRIDYKIVWDVINNFIPN
                     LKTQIVEILSSYLI"
     gene            complement(1781..1903)
                     /locus_tag="PLF66_16420"
     CDS             complement(1781..1903)
                     /locus_tag="PLF66_16420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65678.1"
                     /translation="MALKYVEERLNDYFAGRANRYLEQMNSQDKQSNPKQRTAV"
     gene            complement(2259..3701)
                     /locus_tag="PLF66_16425"
     CDS             complement(2259..3701)
                     /locus_tag="PLF66_16425"
                     /inference="COORDINATES: protein motif:HMM:NF013595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HMZ65679.1"
                     /translation="MRNTVIILFLISTLHCLKLDNKYDTTNTSYWVNTYIYSVVVPRI
                     RSYVSPPSSLSYTNGNNMIFYKGYSNSSLNTNSIKETVSGLTFTASPSLPTGISLNTS
                     TGAISQNSPPSTQASTAYTITGCNSGGCSSISITIRIVNTTATAVYGQAGSFTTSTVN
                     NGGVSANSLYNPEGIAIDSSNGLYVADTLNNRVLYYPSGSTTATRVYGQLGNFSSSTL
                     NNGGISATSLSQPKGVAVDSSGNLYVADSGNNRVLFFSSPSTTATRVYGQGGIFTTNS
                     AGTSSTSLSNPIAIATDSTGVYISDYTNNRVFYFLGTSTTPSKVYGQNNSFTSTTSPS
                     TNTLNSLGNPAELVADSSGGLYVIDTFRSGVLYFPSGITTPTKFIGGGETLSTSFTVS
                     GSELSTPRGIALASDGSLFVGAFGSYLVLNYGAGTATKAYGFGIGGNLSASGITQTSA
                     DYFLGPTSVALDSSSTVYICDKSGNRILKF"
     gene            complement(3711..5723)
                     /locus_tag="PLF66_16430"
     CDS             complement(3711..5723)
                     /locus_tag="PLF66_16430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65680.1"
                     /translation="MIFKNKKFYLLCFLFFFIFSTSIFSEDKKKSVYIGSVQSLNLPV
                     GTDTRVRNGITLSLINNFKNKVAVLDDEVVKKLLERLKLQQLTGCSTEKCEKQLNDAL
                     NADQKIEGTIINEGGKYSLTIKLFNLQDGTPSITNQVKREFTSSQLDYYINELTRSAI
                     DSNYSINDKNAPQDNDPGKINLSNVVLKDVVGSDLSVLNFKSDDTTVDDLIKFSKETL
                     QEGDESFKKKNYDLALKDYESILKGIARLAPEKQNAAKKYKDEIVKRIDNCYINIYNK
                     SLGEIDVKIKSETSATPAKIKDFAEAYEKILLDYQGRIKLLNVPTNPNIESAIVDRLE
                     KLDIANATYSEKKADLQYENYRFSQAIREYKKIYTIIASRPQTKSYKEYLAKLQKKIE
                     ATKETGASFVDNRVKAYCSMAEKKNFQRQLAVDKGKTFEVNDAEDSIKDSLLDARKTL
                     QKSEFASEQLFDFYNKTVERINSDKRESLGKRIEKTDVDLEESQEITKEIAIRRSLLF
                     PGYGQYSERPDKLKGKIMVGAGFVLVGSIFLTGVNYYVQQKAYNNLEGTSPAYFAGFS
                     QYSSIFAYNDYQKSHSARSNVIGAYSFFTTSLFLYAALYVYNVLDIIFFTDRKEVLGL
                     NTFQKTLMASINQSGFNFNISRVNTALGNSNSNLEQKYNFSYTYQW"
     gene            complement(5720..6208)
                     /locus_tag="PLF66_16435"
     CDS             complement(5720..6208)
                     /locus_tag="PLF66_16435"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HMZ65681.1"
                     /translation="MKTIKILLIFFISLELVSSEVHNLPVYDLQDERYTIHSTLSNFT
                     KEKLLLINFTNSNCKPCKIEIPKLLQLHEKLSSQGLVLWIVFVGDNNDTIEPLVKEFG
                     IGDNVKVMKDPLEISYKRMKFTGVPANFIVSKNKEISYTSIGYTDQKQKALEKKVEEL
                     MK"
     gene            complement(6765..7550)
                     /locus_tag="PLF66_16440"
     CDS             complement(6765..7550)
                     /locus_tag="PLF66_16440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65682.1"
                     /translation="MTKGKNKPQTRKPIASLAAKYNHFIKNKIPTRIHMSLILLSVSL
                     TGMLTSRILLELGFSVLWIRYLITSILCYLFFLLYIKIWLLYINPGKNQRKDGSVGNL
                     DLSGIDINIESGESVKISEMLGQGGNFGGGGASASFDGGSSSSILPDIGGVDLDIDGD
                     AAAPILIVVVVGIAVIVIFGGAVFIVYQAPAILSEAAFEGLLATGFFKSTRDISSGNW
                     VGSILKRTWIPFLLISTVATGSGWLIGYLCPIATKIFDISKCL"
     gene            complement(7618..8142)
                     /gene="rplQ"
                     /locus_tag="PLF66_16445"
     CDS             complement(7618..8142)
                     /gene="rplQ"
                     /locus_tag="PLF66_16445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004779232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HMZ65683.1"
                     /translation="MNKRNKVKQLNRTHEHRQAMLNNMVTSLFKHERIESTVAKIKVV
                     RSFAEKLISRAKKNIGEGKQEVALHNKREVMKKIKDRDVVVKLFEDIAPRFSGRNGGY
                     TRIVRLVNRPSDNSEMGILELVDRKSKEELKEAKLAKSKALAEKKAAAKAARKSGTGV
                     KDATVATKKGKEKK"
     gene            complement(8143..9120)
                     /locus_tag="PLF66_16450"
     CDS             complement(8143..9120)
                     /locus_tag="PLF66_16450"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010573768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HMZ65684.1"
                     /translation="MSHKNLLKGFKRPKKIEYYTEVNTPNYGKFIAEPFERGFATTLG
                     NSLRRTLMSSIEGSAISAIRIEGVTHEFSYLEGISEDVSRIILNLKQVRIKYEPEDRD
                     MNKIIHIELKGAGYFKAGDLAVDSSIEIMNPDLLIATLNEDANLVMDIEIQRGKGYFP
                     AEEKKKDIEVLGTIPVDSLFSPIQKVLFEISETRVAQRSDYEKLTLEIWTDGSVSPED
                     ALAQAAKILKEHLTIFINFEEEVEEEVDELDEADQKMKVALSKHVEELELSVRSLNIL
                     RSLEIDFVGDLVKRNEDEMTKSRHYSDECLNELKAKLATLGLSFGMRDF"
     gene            complement(9139..9771)
                     /gene="rpsD"
                     /locus_tag="PLF66_16455"
     CDS             complement(9139..9771)
                     /gene="rpsD"
                     /locus_tag="PLF66_16455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004282060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HMZ65685.1"
                     /translation="MARYTGPVCKLCRREGLKLFLKGSRCLNKDKCSFEDRKNPPGPP
                     PKKKGKISEYSTQLREKQKVKRIYGVLEKQFRDYYEKASHLEGITGETLLQLLERRLD
                     NVLYRMGFASSRAQSRNFIGHGHVLVNGHKVDIASYQVQIGDKVSFKEKLSKTDMMDQ
                     NIKLAQSLNRHPGWVTPDYTKFVGEITSLPAREHVDMPIKEQVIVELYSK"
     gene            complement(9786..10220)
                     /gene="rpsK"
                     /locus_tag="PLF66_16460"
     CDS             complement(9786..10220)
                     /gene="rpsK"
                     /locus_tag="PLF66_16460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HMZ65686.1"
                     /translation="MEKEVKDSKDKKDKKEKKVKKVKKKEKKNVPKGKVYIQASFNNT
                     IVTITDMAGNVLAWSSSGLMSFRGSKKSTPYAAQIAAGNAADKAIDAAGLKEVDVLVS
                     GPGIGRESAIRSLASKGLAIKIIKDITPLPHNGCRPRKRRRV"
     gene            complement(10248..10628)
                     /gene="rpsM"
                     /locus_tag="PLF66_16465"
     CDS             complement(10248..10628)
                     /gene="rpsM"
                     /locus_tag="PLF66_16465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010573766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HMZ65687.1"
                     /translation="MARFAGIDIPKDKRIVVGLTYVYGIGRSSSRKILAKAKISEDVQ
                     VKDLNDDQENAIRRVIEEDGYLVEGDLRSDINLNIKRLMDIGCYRGLRHRKGLPVRGQ
                     RTKTNARTRKGGKKTVANKKKVTK"
     gene            complement(10632..10745)
                     /gene="rpmJ"
                     /locus_tag="PLF66_16470"
     CDS             complement(10632..10745)
                     /gene="rpmJ"
                     /locus_tag="PLF66_16470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HMZ65688.1"
                     /translation="MKVRTSVKKICEHCKIIRRKNVIRVICTNPKHKQRQR"
     gene            complement(10753..10971)
                     /gene="infA"
                     /locus_tag="PLF66_16475"
     CDS             complement(10753..10971)
                     /gene="infA"
                     /locus_tag="PLF66_16475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HMZ65689.1"
                     /translation="MPKEEAITVDGTVMEPLPNAMFRVELENGHKILAHISGKMRMHY
                     IRILPGDKVTVELSPYDLTKGRITYRKK"
     gene            complement(10973..11536)
                     /locus_tag="PLF66_16480"
     CDS             complement(10973..11536)
                     /locus_tag="PLF66_16480"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010573765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HMZ65690.1"
                     /translation="MKRLIFMGPPGAGKGTQAKIICKEYNIPQISTGDILRESIKNST
                     EAGLRAKKFMDAGDLVPDEVVIQIIKDRLKDADCKHGFLLDGFPRTIEQAKALDVLLK
                     DLGIALDAVVNLAVPDSELLARLLERAKIEGRADDNEETIKNRLTNYNNKTFPLLDYY
                     RNHKLLKEINGSGKLDEITQLIKKELS"
     gene            complement(11543..12916)
                     /gene="secY"
                     /locus_tag="PLF66_16485"
     CDS             complement(11543..12916)
                     /gene="secY"
                     /locus_tag="PLF66_16485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016547193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HMZ65691.1"
                     /translation="MLNVITNIFKIPELRSKILFTVFMLLLFRMGTHVTIPGINGLVV
                     SGITSDSTGGLLGMVDLFAGGALLKFSIFALGIMPYISSSIIMQLVMVLIPQLQKLQK
                     EGEEGRKKINQYTKFGTIILCIVQSLAVIYMAKGWSTGQGLSPAKYPGLINPYVQSFF
                     VPLGILTITTGTILLMWMGEQITERGIGNGISLIIFAGIIGRMPEAFLQLFTTDKADP
                     LPILILFIIFIFLIYFTIYLTQGTRKVPLQYGKQMVGRRMVQAKSQSIPFKVNGANVM
                     PIIFASSLILFPQTIVQWLSSNGGQWAGWAVLLDFFNPFSTIWYRSLFYYFCYTGLII
                     FFAYFYTAIQFNPQELAENLKKYGGFVPGIRPGTDTKVYIEKVLNRITLPGSIFLAML
                     ALSPYIIIRFLDLGNNTGGGALVYTFGGTSLLIMVGVTLETLKQVEAQLLMRNYDGFM
                     KKSKVKR"
     gene            complement(12920..13462)
                     /gene="rplO"
                     /locus_tag="PLF66_16490"
     CDS             complement(12920..13462)
                     /gene="rplO"
                     /locus_tag="PLF66_16490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016550805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HMZ65692.1"
                     /translation="MSKRKTSNRIQPSAAFGADRKKNKKAADSSNLIPVPKGAKKERK
                     RIGQGPGSGMGKTSTRGQKGQKARASSMRRGFEGGQMPLHRRIPKRGFRNIFHVIYQP
                     VNLGDLTRYNLSGTVTPEILAEKGLIKDAAGLIKVLGTGDLKGAVAITADACSKSALE
                     KIQKSGGSFNARVVAKPAEK"
     gene            complement(13466..13648)
                     /gene="rpmD"
                     /locus_tag="PLF66_16495"
     CDS             complement(13466..13648)
                     /gene="rpmD"
                     /locus_tag="PLF66_16495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HMZ65693.1"
                     /translation="MMDKIKVTQIKSAIGRIPAHRKTLKALGLRKIGSSRVHNKTPEI
                     LGMVDSVKYLVNVEKA"
     gene            complement(13645..14157)
                     /gene="rpsE"
                     /locus_tag="PLF66_16500"
     CDS             complement(13645..14157)
                     /gene="rpsE"
                     /locus_tag="PLF66_16500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016560639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HMZ65694.1"
                     /translation="MAFMDEEVKEFSEKVVKIDRVAKVVKGGRRFSFNALTVVGDSKN
                     RIGIGFGKANEVPDAIRKSIESAKKNLIKINVIGHTIPHEVVGKFKSARVYLKPSSPG
                     TGIIAGESVRSVVEKAGVQDILSKSYGSGNPLNIVKATYEALKKLETPVIAAKKRGIP
                     LKKLFGQGEI"
     gene            complement(14158..14526)
                     /gene="rplR"
                     /locus_tag="PLF66_16505"
     CDS             complement(14158..14526)
                     /gene="rplR"
                     /locus_tag="PLF66_16505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002748489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HMZ65695.1"
                     /translation="MINKQKHRPGQERRIERARFKLKTNGDRPRLVFNKTNRYLIAQV
                     IDDKVGSTIAYAVTSEKTFPIKGYSKKNKQSATELGRMIAERAKSKGVTKVMLDRSGI
                     IYHGNLAAFAESARKEGLEF"
     gene            complement(14526..15065)
                     /gene="rplF"
                     /locus_tag="PLF66_16510"
     CDS             complement(14526..15065)
                     /gene="rplF"
                     /locus_tag="PLF66_16510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020781891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HMZ65696.1"
                     /translation="MSRVGKSIITLPAKTEVKQAGDKIEIKGPLGTLSTPLYKGISMS
                     VEGNVVKFTRANEEKQTIALHGLVRALFNNCVKGVTTGWEKNLEIFGVGFRAQKKGSN
                     LVMNLGFSHEVIFPEPPGIKIDVADQLKIKISGVDRQLVGQVAANIRGLKEPEPYKGK
                     GIKYANEYIKRKAGKTGKK"
     gene            complement(15090..15488)
                     /gene="rpsH"
                     /locus_tag="PLF66_16515"
     CDS             complement(15090..15488)
                     /gene="rpsH"
                     /locus_tag="PLF66_16515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004779233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HMZ65697.1"
                     /translation="MSLSDPIADMLTRIRNAGSAKHPTCKVAGSKLKNSILAIMKDEG
                     FIADFQESKANNVPEYTITLKYDDTRRPVIREIERVSKPGRRVYIKSEDVKPVKSNLG
                     IDILSTSKGIMTNKKAMKLKIGGEVICRVS"
     gene            complement(15508..15693)
                     /locus_tag="PLF66_16520"
     CDS             complement(15508..15693)
                     /locus_tag="PLF66_16520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HMZ65698.1"
                     /translation="MAKTSIVARQNKKPKFKVRTYNRCPLCGRPRAYMRRFGMCRICF
                     RELASTAQIPGVTKSSW"
     gene            complement(15711..16259)
                     /gene="rplE"
                     /locus_tag="PLF66_16525"
     CDS             complement(15711..16259)
                     /gene="rplE"
                     /locus_tag="PLF66_16525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020770483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HMZ65699.1"
                     /translation="MEQSRLKKKYNQEIKPALMKKFNFKSVSQVPKLEKIVLNVGMGE
                     GHSNPKMLESALDTLAAITGQKAVKTIAKKSIAGFKLRENMNIGCMLTLRGEKMFEFL
                     DRFINVALPRVRDFKGVNPKGFDGRGNYNLSIKEQIIFPEIQFDKVHSVHGMNITVVT
                     STKSDEEAYELLAGFGMPYRKK"
     gene            complement(16262..16609)
                     /gene="rplX"
                     /locus_tag="PLF66_16530"
     CDS             complement(16262..16609)
                     /gene="rplX"
                     /locus_tag="PLF66_16530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012388931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HMZ65700.1"
                     /translation="MATKLPYRGSEPTKFKKIKLKKDDEVICIAGADKGKKGKVLSFD
                     KRKDRVFVSGINKRKKYVRPTQENPQGGIIEIENSIHISNVMLYDSKKKKGARVGFEV
                     KNGSKERTLRAKG"
     gene            complement(16611..17003)
                     /gene="rplN"
                     /locus_tag="PLF66_16535"
     CDS             complement(16611..17003)
                     /gene="rplN"
                     /locus_tag="PLF66_16535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HMZ65701.1"
                     /translation="MIQMQTFLQVADNSGVKRVQCVKVLGGSKKRYATIGDEIIVAVK
                     DSQPAYGLRDSIGKKVHAKAVQRAVVVRTRKEIRRPDGSYIRFDDNAVAIIDDKGNPK
                     GTRIFGPVARELRDKKFMKIISLAPEVL"
     gene            complement(17005..17271)
                     /gene="rpsQ"
                     /locus_tag="PLF66_16540"
     CDS             complement(17005..17271)
                     /gene="rpsQ"
                     /locus_tag="PLF66_16540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020770423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HMZ65702.1"
                     /translation="METKVEVKKRKTIQGKVVSTKMAKTIVMEVEVRQNHPMFKKITR
                     KTSRIKVHDEQGECKEGDVILAMETRPLSREKRHKLLKIVERAK"
     gene            complement(17310..17501)
                     /gene="rpmC"
                     /locus_tag="PLF66_16545"
                     /pseudo
     CDS             complement(17310..17501)
                     /gene="rpmC"
                     /locus_tag="PLF66_16545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004764922.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
     gene            complement(17498..17911)
                     /gene="rplP"
                     /locus_tag="PLF66_16550"
     CDS             complement(17498..17911)
                     /gene="rplP"
                     /locus_tag="PLF66_16550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010573755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HMZ65703.1"
                     /translation="MLAPKRVKYRKWHIGRLKGKDEVANKVSFGEYGLMAVSSGRITA
                     RQIEAARVSINRRVKRGGKLWIRIFPHVPVTKKPAETRMGKGKGNPEFWVSEVRPGRV
                     LFEMSGIDEASAKKALTLASYKLPIHTEFVKRNLI"
     gene            complement(17930..18607)
                     /gene="rpsC"
                     /locus_tag="PLF66_16555"
     CDS             complement(17930..18607)
                     /gene="rpsC"
                     /locus_tag="PLF66_16555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HMZ65704.1"
                     /translation="MGQKVNPIGLRVGITRTWDSIWFAKEEYTKNLHEDIKIREFLQN
                     KFKNSSLVKVVIERFPEKINVNLHTAKPGVVIGQKGQNIENVKSLLKPMSDKPININI
                     IEVKKPETVAQAIAETIATQIEQRMPFRRVMKAELRRAMRGGVEGIKISISGRLNGAD
                     MARTESYKDGRIPLHTLRAKIDLGTKEALTTFGQIGIKVWTYSGDHISTSNDEVEDKY
                     AVKRRTN"
     gene            complement(18622..18954)
                     /gene="rplV"
                     /locus_tag="PLF66_16560"
     CDS             complement(18622..18954)
                     /gene="rplV"
                     /locus_tag="PLF66_16560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010573753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HMZ65705.1"
                     /translation="MESKAVAKYVRVSARKARLVANEVRGYDYTEAVDILKFTQKRVA
                     DEILNVVNSAAANAMVADPKIDLSQVYIKKIYVEDGPIMKRFKARARGRAARIRKRLS
                     HITVVLSN"
     gene            complement(18964..19242)
                     /gene="rpsS"
                     /locus_tag="PLF66_16565"
     CDS             complement(18964..19242)
                     /gene="rpsS"
                     /locus_tag="PLF66_16565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002775069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HMZ65706.1"
                     /translation="MSRSLKKGPFIDDHLMRKITKMNSEGNKKPFKTWSRRSTIFPDM
                     IGHTVMIHNGNKFIPVYINDNMVGHKLGEFAPTRTFRGHSGSDKKVKK"
     gene            complement(19246..20091)
                     /gene="rplB"
                     /locus_tag="PLF66_16570"
     CDS             complement(19246..20091)
                     /gene="rplB"
                     /locus_tag="PLF66_16570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000511540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HMZ65707.1"
                     /translation="MGIRKFKPVTAATRYKSVLDFAEITEERPHKPLTTTVKYNAGRG
                     DGGKISVRRKGGRVKRKYRIIDFRRNKYGIPAVVKTIEYDPYRTSFIALLSYKDGEFS
                     YIIAPEGLKVGDVLVAGESAEIKVGNSLPLEKIPPGTDVHNIELHRGKGGQMARTAGS
                     FATVSAKDGDYVTLKLPSSEVRKVRKECYATIGIVGNKDHNLVSIGKAGRSRWLGRRP
                     KVRGVVMNPVDHPHGGGEGKTSGGRHPVSPWGQPTKGYKTRRKARPSDKFIVQGRKRN
                     RNRGN"
     gene            complement(20094..20402)
                     /gene="rplW"
                     /locus_tag="PLF66_16575"
     CDS             complement(20094..20402)
                     /gene="rplW"
                     /locus_tag="PLF66_16575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002722983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HMZ65708.1"
                     /translation="MNIDSIIIAPIITEKSQSAQEIGKDIGKRTVKYTFKVHPDANKV
                     MIKEAIKKIFQVTPSSVNVIMYHGKMKKFRNFPARRPHWKKAVVTFDNGANLEFGKGV
                     "
     gene            complement(20399..21034)
                     /gene="rplD"
                     /locus_tag="PLF66_16580"
     CDS             complement(20399..21034)
                     /gene="rplD"
                     /locus_tag="PLF66_16580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002999914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HMZ65709.1"
                     /translation="MKARKYSKTGQSLAEVELPGEIFTSKVSSGAIYDAIKAENANLR
                     SGNHATKDRGEVRGGGKKPWSQKGTGRARQGSSRAPHWVGGGTVHGPKPRDYSIPLSR
                     NVKRVAVVSILNKKASESKINILEDIAMTKYSTKEIFNIFKTMGVTNGAFSLVVSGED
                     KFLKASTKNIPTLKYVNAKRIVCRDILYNNQLIITESALKEIVSQYKDVSK"
     gene            complement(21053..21673)
                     /gene="rplC"
                     /locus_tag="PLF66_16585"
     CDS             complement(21053..21673)
                     /gene="rplC"
                     /locus_tag="PLF66_16585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020765762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HMZ65710.1"
                     /translation="MTKGLIGKKLGMSHIYADNGNMIPVTVIEVGPCFVSQVKTVESD
                     GYSAVQLAFGNARETQLSKAEVTHLAKNNIAPKRYLKEFALTGDAPAAGSEIKIADIF
                     KDNDRVKVTGISKGKGYQGVVKRHGHSGGPAAHGSRFHRHPGSMGANSTPSRVFKGVK
                     LPGRTGSIKTSIKNLRVVKVYEDKNILLVSGSVPGPEKSIITIEKL"
     gene            complement(21683..21991)
                     /gene="rpsJ"
                     /locus_tag="PLF66_16590"
     CDS             complement(21683..21991)
                     /gene="rpsJ"
                     /locus_tag="PLF66_16590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008593919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HMZ65711.1"
                     /translation="MTGQRIRVKLRAFDHKLVDQSTYEIVATAKRTGATVSGPIPLPT
                     KVEKFTILRSPHVNKKSREQFEMRTHKRLIDILNTNEDTVEALMKLQLPAGVSVDIKS
                     "
     gene            complement(21996..23210)
                     /gene="tuf"
                     /locus_tag="PLF66_16595"
     CDS             complement(21996..23210)
                     /gene="tuf"
                     /locus_tag="PLF66_16595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011669431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HMZ65712.1"
                     /translation="MAKEKFDRSKPHLNVGTIGHVDHGKTTLTAAITSTLAKVIGGKN
                     KAVKYDEIDNAPEERERGITIATSHQEYETVNRHYAHVDCPGHADYVKNMITGAAQMD
                     AAILVVSATDGAMPQTKEHILLARQVGVPYIVVFLNKADQLKDDERAEMIEMVEEEIR
                     DLLKKYNFPKEGQGPIPIIHGSALKALEGEDSELGTKAVLKLMEALDTYVPNPKRVTD
                     KPFLMPVEDVFSITGRGTVATGRVETGVLKINEEVEIVGIKDTAKTVVTGIEMFRKLL
                     DQAEAGDNIGALLRGTKKEEIERGQVLAKPGTITPHKKFTAEVYVLTKDEGGRHKPFF
                     NNYRPQFYFRTTDITGVCVLPAGMEMVMPGDNVSVTIELIHPIAMDKGLNFAIREGGK
                     TIGSGVVAEIIE"
     gene            complement(23255..25129)
                     /locus_tag="PLF66_16600"
     CDS             complement(23255..25129)
                     /locus_tag="PLF66_16600"
                     /inference="COORDINATES: protein motif:HMM:NF012239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HMZ65713.1"
                     /translation="MQRNIRNIGIFAHVDSGKTTLTERILFEAGEIYSPGSVLEGTTE
                     SDRMREEIERGISIVATTIQIRYKYKNKKYSINLIDTPGHLDFYAQVRNSLLAVDIAV
                     LLLDITTGIRSQTEMLLEEITELGIPLIIFINKIDKAENLKNFILELNESLPRKLNPL
                     FTITDGFTNQIEYTFLKKEIQEDIELSFIEWNDSLVEKYFKKPSKKVLLEGLYSGFKE
                     SKLTPLFAGSASHGASVKELLDFICETDFHRRHSQDLDCRGVLFKRELHPDLGKIYFI
                     KAYEPMRIGDIYFSKEKSFKLESFYEITPNEITKLDIMDGYSIFAATISETSHVSTGS
                     LFLEKKEEANVVSSHNNEYVLLLEPNKSEEKETLRSGLEKLVWEDSGLSFKEKADTGQ
                     FELRGAGELHLDVAIQRLKEFISEIFTFGNLKVARYEKFKNMAITVIFEHSAFNQKLK
                     SGTLISSLEKSPDFSSSVLWECELEEDYKSSVESAFYEVLSNGSRGFPVLGVNLRVLS
                     FERPEVVNDFTSNLLKVAIISGIKSYLPECTIQIGPLCQFEVVIPESYYGSVLAVLGK
                     RNAKIVKTDIVHGNKSLLICEAAAENMLGFTSALRNMTKGKGFLSQKTIFTSLSHFEF
                     "
BASE COUNT         7383 a         5105 c         3929 g         8957 t
ORIGIN      
        1 caatatgaga agcaatttgc ctaaaatcgt gtccactaaa tatgtagtag ttcatttcat
       61 aatgacaccg attcctgtaa tagcaactcc tgcaattcct aagccactag ctgcagctgc
      121 tgcaaaatca cctccaaaag ccattgacca gagttttgca aagccaccac tcgccccgac
      181 tccacctcct aatccttcgt aacccgaacg tatcctatca tagccacctc ttactccaaa
      241 ttcataaatc ccagtggcta ataagcataa gcctgtcaat ggtgccgctc caagtctaac
      301 agcatatcca taaggaccag caacgccaca atgtgagtaa tttactcttg attgctttgc
      361 ataatcctga ttagtcattt tgttaaattg atccgcagca ccttttagtc caccgctcat
      421 ccatcttacc gtgtgatcga ttccgctacc tgcccagcgt gcaccatttc caattccagt
      481 tcctgcccat ctcgctccac ctgtaatatc cttcgctaca cttcctgtgc tgtgtttgcc
      541 acctgccaaa tacatcccga tcgccgctcc ttctgaacca gagaatggtg acccagattg
      601 tgaagcaaaa aaatatcccg ctatcgctcc agccattgcg gatactcctg catgtccgct
      661 tgggtcacgc acattagttg gcgaaccact cacatacata taccgattca tcccaaacat
      721 agactccggc ataacaatac tatcagcttg caggaatctt cctaaaacag gatcataata
      781 ccttgcttta taatacatca aacgtttatg accataaaaa gaataatatt taaaaaaagc
      841 aatagaattt tataaatcat actaaggaat tctagatatg aaagttttta catacatcgc
      901 ataaactcca tttcaacttc tgctcattga gaacttacaa aaactatatt tttaaggaaa
      961 cactctgccc tataaaaata cttgcttttt tatagaatag agatcgttat atatacatga
     1021 tttcggtaaa catagaaccc aatcagatat ccaatatacg tgatgtagta aaaggatatt
     1081 ttcaaaccaa acctgtaaat aaagtgtatc tattcggatc gtatgctcgg aaagaagctg
     1141 gaaagaatag cgatatagac ctactccttg atttgattta tgaacccggt attgctttta
     1201 tttttggaat gatgaaacaa gagttagagt tgttgttaca taaaaaagtt gatttaataa
     1261 caaccaattc tatatcaaaa tacatcgccg agaatataga aaaagagaaa atccttatct
     1321 atgaaaaaat ttaattcaga taaaatctgg cttgaatcca ttctcgaaaa tattcactct
     1381 ttagaaagtt tctctgctgg tatgtcgtat gaaatttttg atagggattt aaaaactaaa
     1441 tttgcttgtg aaaaatgtat ccagaatatt gctgaagcgt caaacaatct ttctttagaa
     1501 ttcaagcaag aaaataaaaa ttttccatgg agtcaaatga aagcaatgcg aaatatcata
     1561 acgcatgaat actttcgaat cgattacaag atagtttggg atgtgattaa taattttatt
     1621 cctaatttaa aaactcaaat tgtagaaatt ctaagttctt atcttattta atgtgaattc
     1681 gatgtaaatt ttttcaccat agatacagaa acgattgata attttgaatt aaacattgta
     1741 aaaaaagaac tgacacttca aatcatacgg ctaatcgtta ttataccgca gttctttgtt
     1801 tagggttcga ttgtttatcc tgagaattca tttgttctag atagcgattt gcgcgacctg
     1861 caaaataatc attgagcctt tcttccacat atttgagtgc catacagaaa atcgctccag
     1921 cccttcacac tatttgtcat cgaatgtttc gtatattgca tgttataaat ttatttatag
     1981 attacattta tgaatcatta ttataacttc ttattgcttt caatctactc aattaaattc
     2041 atcgttaccc caattgtgtt catttgattt ggatattaaa tcattagtag agatatatct
     2101 atttttcagc actgtttcat tgcagatgca gaatcaaagg attccctatg ttgcttattt
     2161 tcgcatcttt cacttttagg acgatctaaa caactatgat tcttccaaat acaaataacg
     2221 accattaccc gctgatgaaa aaacagtcat gcatacaatt aaaattttaa aatgcgattc
     2281 cctgatttgt cacaaatata aacagttgag gaactatcga gtgcaacaga agtaggacct
     2341 agaaaatagt cagctgaagt ttgagtaata ccactagctg acagattacc tccaatccca
     2401 aaaccatatg ctttggtcgc tgtgcctgct ccataattta atacaagata ggagccgaac
     2461 gctccaacaa ataaggaacc atctgaagca agtgcgatcc ctcttggcgt tgaaagctca
     2521 gatccactaa cggtgaaaga ggtggataga gtctctcctc ctccaatgaa tttagtagga
     2581 gttgtaattc ccgaaggaaa atacaacact ccggaacgaa atgtatcaat cacatagaga
     2641 ccaccggacg aatcagctac aagctcagca ggatttccaa gagagtttaa agtatttgta
     2701 gaaggtgaag tggttgaagt aaagctatta ttttgtccat agactttaga aggagtcgta
     2761 ctcgttccca aaaaataaaa aactctgtta ttagtataat cagaaatata aacacctgtg
     2821 ctatctgtcg caatagcgat tggattactt aaactagtcg atgaagttcc tgctgaatta
     2881 gtagtaaaaa ttcctccttg cccataaaca cgggtagcag tcgtgcttgg cgacgaaaaa
     2941 aataaaaccc tattattacc agaatctgct acatataaat ttccagaact atctactgcg
     3001 acccctttag gctgacttaa actagtggct gaaattcctc cattatttaa agtgctactt
     3061 gagaagttac ctaattgccc gtaaactcga gttgccgttg tgcttccaga tggataataa
     3121 agcacgcggt tatttaatgt atccgctaca tataaaccat tactcgaatc tatcgcaatt
     3181 ccttctggat tgtacagact attagcactc actccaccat tgtttactgt gctagtagtg
     3241 aaacttccag cttgtccata aacggcagtc gctgtagtgt ttacaatgcg aatggtaatt
     3301 gaaatagaag agcatcctcc tgaattgcaa ccagtaattg tataggctgt agatgcttgt
     3361 gtagagggag gagagttttg actgattgcg cctgtcgatg tattaaggga tattccagtt
     3421 ggcagagaag gacttgcagt aaatgtcaat ccggacacag tttcttttat actatttgtg
     3481 tttaaagaag aattactgta tcctttataa aaaatcatat tattcccatt cgtataactg
     3541 agagatgacg gaggagaaac ataacttcgt atccgaggaa ctacaacact ataaatatat
     3601 gtatttaccc aataacttgt attggtagta tcatatttat tgtctaactt tagacaatgt
     3661 agagtagaaa ttaaaaaaag aatgattact gtgtttctca ttgggttctc ctaccattga
     3721 taagtataag aaaaattata cttttgttct agattcgaat ttgaatttcc taatgcggta
     3781 tttactcgac ttatattaaa attgaaacca ctttggttta tacttgccat taatgttttc
     3841 tgaaacgtat ttaaacccaa tacttcttta cggtctgtaa aaaagataat atccagaacg
     3901 ttatacacat agagtgcagc atataaaaat aaactcgttg taaaaaagga ataggctcct
     3961 attacattgg aacgagccga gtgagatttt tgataatcat tgtatgcaaa tatcgaacta
     4021 tattgtgaaa aacctgcaaa ataagcaggc gacgttcctt ctaaattatt ataagctttc
     4081 tgctgcacat aataatttac acctgtcaaa aatatggacc caactaaaac aaatccagca
     4141 cctaccataa ttttcccctt taacttatcc ggtctctccg agtattgtcc atagccggga
     4201 aataagagtg atctacgaat cgctatctcc ttcgttattt cttgactctc ctctaaatct
     4261 acatctgtct tttctatacg ttttccaagg gattctcttt tgtcagaatt aattctttct
     4321 actgttttat tataaaaatc aaaaagttgt tcagaagcaa attctgactt ttgaagagtt
     4381 ttcctagcat ctaaaagaga atctttaatt gaatcctctg catcattcac ttcaaatgtt
     4441 tttcccttat caacagccaa ttgtctttgg aaattctttt tctctgccat actacaatat
     4501 gctttaactc tattatcaac gaaagaggcg cctgtttctt tagtagcctc gattttcttt
     4561 tgaagcttag ctaaatattc tttatatgat tttgtttgtg gtcttgatgc aataattgta
     4621 taaatctttt tatattctcg aatcgcttgg ctaaatctat aattttcata ttgcagatcc
     4681 gcctttttct ctgaataagt tgcatttgca atatctagtt tttctaaacg atccacaata
     4741 gcagattcaa tatttggatt ggttggaaca ttcagtaatt taattctacc ttgataatca
     4801 agtaaaattt tttcataagc ttctgcaaaa tcttttattt tagctggagt agccgatgtt
     4861 tctgatttta ttttaacatc aatttcacct aacgatttat tatatatatt tatatagcag
     4921 ttatcaatcc tctttacaat ttcatcttta tatttttttg ctgcattttg tttttccggt
     4981 gctaatcttg ctataccttt taaaattgat tcataatctt ttagagcaag atcataattt
     5041 ttcttcttaa aactttcatc accctcttgg agagtttcct ttgaaaattt aattagatca
     5101 tccacagttg tatcatccga tttaaaattt aaaactgata aatctgatcc aactacatct
     5161 tttaaaacta cattgcttaa attaattttt ccaggatcgt tatcctgggg tgcattttta
     5221 tcattgatgg aataatttga atctattgca cttcgagtta attcatttat ataatagtcc
     5281 aattgagatg aggtaaattc tctttttact tggtttgtga tactcggagt tccatcctgt
     5341 aaattaaata acttaatggt taatgagtat ttgcctcctt cgtttataat agtgccttca
     5401 attttttgat ccgcattcaa tgcatcattt aattgttttt cacatttttc agtggagcat
     5461 ccagtcaatt gttgtagttt taatctctct aataattttt taacaacctc atcatcaagg
     5521 acagctactt tatttttgaa gttgttaatc aaagaaagag taattccatt tctaactctt
     5581 gtgtctgtcc caacaggcaa atttaaggat tggacagaac ctatataaac agatttcttt
     5641 ttatcttcag aaaaaataga agtagagaat ataaaaaaga aaaggaagca tagcaaatag
     5701 aattttttat ttttaaaaat catttcatca attcctcaac tttcttttcg agagcctttt
     5761 gtttctgatc agtataacca attgatgtat aggagatttc tttattttta gaaactataa
     5821 aattagcagg aacaccggta aacttcattc ttttataaga aatttctaag ggatctttca
     5881 tgactttcac gttatcacca attccaaatt ctttaacaag tggttcaata gtatcgttat
     5941 tatctccaac aaaaacaatc caaagcacta aaccttgaga agataacttc tcatgaagct
     6001 gaagtaattt aggaatttct atcttacaag gtttgcaatt gctattcgta aaattgatta
     6061 acaaaagttt ttccttcgta aaattactta gagttgaatg aatcgtatag cgttcgtctt
     6121 gtaaatcata tacagggaga ttgtgaactt cactagatac taactctaag gagataaaga
     6181 atattaggag aatttttata gttttcataa gttataagaa aaacttcttc ctaaaattgt
     6241 ctcctttttt tcgtgataga taaatataaa taatattata gatactgtgt ttacatatct
     6301 taatttaaaa attaattatt tgaatacagt tggagagaat aactatattt atatttcaaa
     6361 aaacaaggga tttttcctgc gatgactgtc tggagatcat tcgcgaaatc tggagttatg
     6421 taaaaataga caaaatgtta tcttaaattg tgtttctatc aagtctctct ttttgagaaa
     6481 agattggtaa taatttacca tagagttcgg atccatactt ttagtaagcg attctacaag
     6541 ttacgggtga taatttttac atagccccac taagaagtgt ttaaaccgaa tgcaaattta
     6601 atctaatcat ttggtatgtg tataattcac tccgcctaac ctcaaaaagc aaaaattcta
     6661 tccatttttc aaaagtccca gctagttata aaaaaagtta aggctgctca taaaaaaact
     6721 aaggctagtt ctggaaaagc agccttgaga gttaggtttt cagctcataa gcacttacta
     6781 atatcgaaga ttttagttgc aatcgggcag agatatccta tgagccagcc agaacctgtt
     6841 gcgacagtgg atatcaaaag aaacggaatc caagttcttt ttaaaatact gcctacccaa
     6901 tttccagaac tgatatctct cgtgctttta aaaaaacctg ttgcgagtaa tccttcaaaa
     6961 gcagcctcag ataaaatagc tggagcctga tatacaataa agactgcccc tccaaagatt
     7021 acaatcacag caataccgac aacgactacg attaaaatgg gagctgctgc atctccatct
     7081 atatccagat caaccccacc aatatcgggc aaaatagagg aggaagaacc tccatcaaaa
     7141 ctagcactcg ctccccctcc tccaaaattt cccccctgcc ctagcatttc agaaatcttt
     7201 acggattctc cgctttcaat atttatatca ataccggaca aatctagatt gccaacacta
     7261 ccatcttttc tttggttctt accgggattg atatagagaa gccaaatttt tatataaagc
     7321 aggaaaaaca aataacaaag aatggaggtg attaaatatc gaatccacaa aacagaaaaa
     7381 ccaagttcta agagaattct agaagtaagc attcctgtta aagatacact caatagaatc
     7441 aaactcatat gaattcgagt gggaatttta ttttttataa aatggttgta tttagccgct
     7501 agtgatgcaa ttggctttcg agtctgtggt ttgttttttc cctttgtcat tgtttcctct
     7561 aaaaacaaaa aaagcccgac atccgccggg cttcttacaa aaagggatta gagactttta
     7621 ctttttctct tttccttttt tggtagcaac tgtcgcatct ttaacgccag ttcctgattt
     7681 tcttgctgct tttgcagctg cttttttctc cgcaagtgct ttgctcttag caagttttgc
     7741 ttctttcaat tcttcttttg attttctatc tactaattct aaaattccca tttcagaatt
     7801 atcagaaggt ctatttacaa gacgaacgat tctagtatat ccaccatttc ttccagagaa
     7861 tcttggagcg atatcttcaa aaagttttac cactacatca cgatctttga tttttttcat
     7921 cacttctcgt ttgttgtgca atgctacttc ttgctttccc tcgccaatgt ttttcttagc
     7981 tctggagatc aatttttcag caaaagaacg aactactttg atttttgcga cagtagactc
     8041 aattctttca tgcttgaata aactggtcac catgttgttt aacatagctt gtctatgttc
     8101 atgggttcta tttaattgtt taactttgtt tcttttgttc atttaaaaat ccctcatacc
     8161 gaacgacaag ccaagcgtag ctaatttcgc ttttaattcg tttagacact catcactata
     8221 gtgtcttgac tttgtcattt cgtcttcatt tctctttact aaatcaccca caaaatcgat
     8281 ctctaggctt ctgagaatat tgagtgagcg cactgataat tctaattctt ccacatgctt
     8341 agatagagca accttcatct tctgatctgc ttcatctaat tcatccactt cttcttctac
     8401 ttcttcttcg aagttaatga agatagtgag atgttctttc aaaatctttg cagcttgtgc
     8461 taaagcatct tctggagaaa cagaaccatc tgtccaaatt tctaaggtta atttctcata
     8521 gtcagatctt tgcgctacgc gtgtttctga aatttcaaat aatacttttt gaattggaga
     8581 aaaaagagaa tctactggaa ttgttccaag cacttcaatg tcttttttct tttcttctgc
     8641 tggaaaatat ccttttcctc tttgaatttc aatgtccatt actaggtttg catcttcgtt
     8701 caaagtagca attaaaagat ctgggttcat aatctcaata gaagaatcaa cagccaaatc
     8761 tcccgcttta aaataacctg cacctttcaa ctcgatatga ataattttat tcatatctct
     8821 atcttctggt tcgtatttaa ttcggacttg cttgaggttt agaataatac gagaaacgtc
     8881 ttcgctaatt ccctcaagat aagaaaactc atgtgttact ccctctattc taatagcaga
     8941 gatagcagag ccttctattg aagacattaa agttcttcgc aaagaatttc ctaaagtagt
     9001 agcaaaacct ctttcaaaag gttctgcaat aaacttacca taattaggag tatttacttc
     9061 tgtgtaatac tcaatcttct ttggtctttt aaagcctttt aataaatttt tatgagacaa
     9121 agctgtaacc ttccattctt atttagagta taactctaca atgacttgct ctttaattgg
     9181 catgtctaca tgctctcttg ctgggaggct tgtaatctct ccaacaaatt ttgtatagtc
     9241 aggagtcacc caacctggat gtctatttaa agactgtgca agtttaatgt tttgatccat
     9301 catatctgtt ttagataatt tttctttgaa agaaacttta tctccgattt gcacttgata
     9361 agaagcgata tctactttat gaccattcac taaaacatgg ccatgtccga taaagtttct
     9421 tgactgtgct ctagaagatg caaatcccat tctgtaaaga acgttatcta atcttctttc
     9481 taagagttgt aataaagttt cgcctgtaat tccttctaaa tgactcgctt tttcataata
     9541 gtcacggaat tgcttttcta aaacgccata aattctcttt actttttgct tttcacgaag
     9601 ctgtgtagaa tactctgaaa tctttccttt ctttttagga ggaggtcccg gaggattctt
     9661 tctatcttca aaactacatt tatctttatt taaacatcta gagcctttta aaaaaagctt
     9721 tagcccttct cttctacaaa gtttacaaac tggtcctgta tatcttgcca tttaaaatat
     9781 ccttatcaaa ctcttcttct tttacgaggt ctacaaccgt tatgtggaag cggtgtaata
     9841 tctttaataa ttttgatagc gagacctttt gatgctaaag atcgaattgc agactcacga
     9901 ccaattcctg gtccagatac taatacatct acttctttta agccagcagc atcaattgct
     9961 ttgtctgctg cgtttccagc tgcgatttga gcagcatatg gagtagattt ctttgagcct
    10021 ctgaagctca tcagccctga agaagaccaa gctaaaacgt taccagccat atcggtgata
    10081 gtaacgatag tattgttgaa agatgcttga atataaactt tccctttagg aacgtttttc
    10141 ttttctttct tctttacttt cttaactttc ttttccttct tatctttttt gtccttactg
    10201 tctttaactt ctttttccat aaaaattatt ccgagttatc cccactatta tttagttact
    10261 tttttcttgt tcgcaacggt tttcttaccg cccttacgag ttcttgcatt ggtctttgtt
    10321 ctttgtccgc gaacaggaag cccctttcta tgacgcaatc cacgataaca accaatatcc
    10381 atcagtcgtt ttatgtttaa gttaatatca gatcttagat ccccttcaac taggtatcca
    10441 tcttcttcaa ttactctacg aattgcattt tcttggtcat cgtttaagtc ttttacttgc
    10501 acatcttcac tgatttttgc ttttgctaaa atctttctag aagaagaacg acctatacca
    10561 tagacatatg ttagtccaac tacaattctc ttatctttag gaatatcaat cccagcaaat
    10621 cttgccattt tttatctctg cctctgtttg tgttttgggt ttgtgcatat tacacggatc
    10681 acattttttc tgcgaataat cttgcaatgt tcacaaattt tttttactga tgttcttact
    10741 ttcataaatt tcctactttt ttctatatgt tattctacct tttgttaaat cataagggga
    10801 aagctctaca gtcaccttat ctccaggtag aattctaata taatgcattc tcatttttcc
    10861 tgaaatatga gccaatattt tatgcccgtt ttcaagttct acccgaaaca tagcattcgg
    10921 aagaggctcc atcacagttc catctacagt tattgcttct tcttttggca atttaactca
    10981 attccttctt aataagctgc gtaatttcat ctaacttacc agatccattg atttctttta
    11041 ggagtttgtg attcctataa taatctaata atggaaatgt tttgttattg taattcgtca
    11101 gtcgattttt aatcgtctct tcgttatcat ctgcacgccc ttcaatcttt gcgcgctcta
    11161 ataaacgagc caataactct gaatccggaa cagccaagtt cacaactgca tcaagagcaa
    11221 ttcctaagtc ttttagtaaa acatccaaag cctttgcttg ctcgatcgtt ctcggaaaac
    11281 catctaataa aaaaccatgt ttacaatcgg cgtcttttaa tctatctttt ataatttgga
    11341 ttacaacttc atctggaact aaatctccag catccatgaa tttttttgca cggagtcctg
    11401 cttctgtaga atttttgata gactctctta gaatatcccc agttgaaatt tgtgggatat
    11461 tatattcctt acaaataatc ttcgcttgtg ttccttttcc cgctccggga ggtcccataa
    11521 atatcaaccg tttcatagtc ttctaccttt taaccttaga ttttttcata aatccatcgt
    11581 agtttctcat cagtaactga gcttctactt gcttgagtgt ttctaaagta acacccacca
    11641 tgataaggag agaggttcca ccgaatgtat atacaagtgc accaccaccc gtattatttc
    11701 ctaagtctag gaaacgaata ataatgtatg gagacaatgc taacatagcc agaaaaatag
    11761 atccaggcaa tgttattcta tttaacactt tctcgatgta tactttcgta tcagttcctg
    11821 gacgaatccc tggaacaaat ccaccatatt tctttaaatt ctcagcaagt tcttgtggat
    11881 tgaattgaat tgctgtataa aagtatgcaa agaaaataat cagtccagtg tagcaaaagt
    11941 aataaaacag agatctatac caaatggttg aaaatggatt aaagaaatct agtaatactg
    12001 cccaacctgc ccactgtcca ccgttcgagg ataaccactg aacgattgtc tgtggaaata
    12061 atatcaagct ggaagcaaag ataattggca taacgtttgc cccattcact ttaaaaggaa
    12121 tgctttggct cttagcttgc accattcttc tacctaccat ttgctttcca tactgcagag
    12181 gaacctttct agttccttgt gtaaggtaaa tagtaaagta gataaggaat ataaagataa
    12241 taaataaaat caagattgga agcggatctg ctttatctgt tgtaaagagt tgcaagaaag
    12301 cttctggcat acgtccgata atccctgcaa agataatgag ggaaatacca tttccaattc
    12361 ctctttcagt gatttgctca cccatccaca taagtaaaat agttccagtt gtgatagtca
    12421 aaatccccag cggaacaaag aaactttgca cgtatgggtt gattaaacca ggatatttag
    12481 caggcgatag accttgacca gtagaccagc cttttgccat gtatataact gctaaacttt
    12541 gcacaataca gagaatgata gtgccaaatt ttgtgtattg attgattttc tttcttccct
    12601 cttcaccctc tttttgcaat ttttgaagtt gtggaattaa aaccataacc aattgcatga
    12661 tgatagaaga agagatatat ggcatgatac ctaacgcaaa aatagaaaat ttgagtaagg
    12721 caccgcccgc aaataagtca accattccaa gtagaccacc tgtagaatca gaagtgatcc
    12781 cagaaacgac cagaccattg atgcctggaa tcgtaacatg agttcccatt ctaaaaagta
    12841 gaagcataaa gaccgtaaat agaattttag atcttagctc tggaatttta aatatgtttg
    12901 tgattacgtt caacatatat tatttttctg ctggtttagc aaccactctt gcattgaaac
    12961 ttccaccgga cttttggatt ttttccaaag cagatttaga acatgcatcc gctgtgatag
    13021 caactgcacc ttttaaatca cctgttccga gaactttaat caatccagct gcatctttga
    13081 ttaagccctt ttcagctaaa atctcaggag tcacagttcc agataaattg tatcgagtta
    13141 aatcacctaa atttacaggc tgatagataa catgaaaaat atttctaaat cctctttttg
    13201 gaattcttct atgaagtggc atttgaccac cttcgaatcc tcttctcatg ctagatgctc
    13261 tagctttttg tcctttttga ccacgagtag aggtttttcc cattccggaa ccaggacctt
    13321 gcccgattct ctttctttct tttttagcac cttttggcac tggaattaag ttagaagaat
    13381 ctgcagcttt tttgttcttt tttctgtctg ctccgaaagc agcagaaggt tgaatacgat
    13441 tgctagtttt tctcttagac atatattaag ccttttctac attcactaaa tattttacgg
    13501 aatctaccat tcctaaaatc tctggagttt tgttatgaac tctagaacta ccgatttttc
    13561 taagccccaa tgcttttaag gtttttcggt gtgcaggaat tctaccgata gcgcttttaa
    13621 tttgggtaac tttgatttta tccattatat ttctccctgt ccaaacaact tcttaagagg
    13681 gataccacgt ttcttagcag cgattacagg agtttctaat ttcttcaagg cttcgtaagt
    13741 tgccttaacg atattcaaag gatttccaga tccatacgat ttagaaagaa tatcttgcac
    13801 tcctgctttt tcaactacag aacgaacaga ctccccagcg atgataccag ttccaggtga
    13861 agaaggtttt aaatacactc ttgcagactt aaatttacca actacttcgt gtggaattgt
    13921 atgaccaatt acattgatct taattaagtt cttttttgca gattcgatag atttacgaat
    13981 tgcatctgga acctcatttg cttttccaaa tccaatacca attctatttt tagaatcgcc
    14041 tacaacagta agagcattga aggaaaatct acgtccacct ttcacaactt ttgctactcg
    14101 gtcgatcttt acgacctttt cgctgaattc ttttacttct tcatccataa atgccattta
    14161 aaactccaat ccttcttttc ttgctgactc tgcaaaagct gctaaatttc catggtagat
    14221 gataccagat ctatccaaca ttacttttgt aacacctttg ctctttgctc tttctgcaat
    14281 cattcttccg agttcggttg ctgattgctt attttttttg gaataaccct tgatcgggaa
    14341 ggttttctcg gatgtaaccg cgtaagcgat tgtgctacct actttatcat caatcacttg
    14401 tgcaattagg tatcgattcg ttttattaaa aactaatctt ggacgatctc catttgtttt
    14461 taatttaaaa cgagctctct cgattcttct ttcttgacca ggtctgtgct tttgtttgtt
    14521 aatcattatt tcttaccagt ctttccagct ttacgcttaa tatactcatt cgcatactta
    14581 attcctttcc ctttataagg ttctggttct tttaatccgc gaatatttgc agcaacttgt
    14641 cctactaact gacgatccac accggaaatc ttgattttta attgatctgc aacatcaatt
    14701 ttgattccag gtggttcagg gaagattact tcatgagaaa agcctagatt cataactaga
    14761 ttagaacctt tcttttgagc tctaaatcca accccaaata tttctaaatt tttctcccaa
    14821 ccagttgtaa cacctttaac acaattatta aaaagggctc ttactaaacc atgcaaagca
    14881 attgtttgct tttcttcgtt tgcacgagta aactttacaa catttccctc aacgctcatt
    14941 gaaatacctt tatacaatgg tgtagaaagt gtcccaagtg gacctttgat ttcaatttta
    15001 tcccccgctt gtttcacttc tgttttcgct gggagagtta tgatactttt acctactcta
    15061 gacataaatt ttagatctct gaagagttat taagaaactc tacaaataac ctctcctcca
    15121 attttaagtt tcattgcctt cttatttgtc atgatacctt ttgaagtcga aagtatatca
    15181 ataccaagat tgcttttaac aggttttaca tcttctgatt tgatataaac ccgtctacct
    15241 ggtttagaaa ctctttcgat ttctctaatt acaggtcttc ttgtatcatc atatttcaat
    15301 gtgattgtat attccggaac attatttgct ttggattctt gaaaatctgc aataaatccc
    15361 tcatctttca tgatagcgag gatggaattt tttaatttgc ttccagcaac tttgcaagtt
    15421 gggtgctttg cacttcctgc atttctgatt ctagtaagca tgtctgcgat tggatctgat
    15481 aaactcatat attaactttc cttctttcta ccaagatgat tttgtaacac ctggaatttg
    15541 agcagtgctt gctagttcac gaaagcaaat tctacacatt ccaaatcttc tcatataagc
    15601 acgaggtctg ccgcaaagag gacaacgatt ataagttctt actttaaact ttggcttttt
    15661 gttttgccga gcaacgatag atgttttagc cataaatctc ttttattctc ctactttttt
    15721 ctataaggca ttccgaagcc agcaagcaat tcatatgctt cttcgtcgga ctttgtgctt
    15781 gtaacaacag taatattcat accatgcaca ctatgaacct tatcaaactg aatctctgga
    15841 aagataattt gctctttaat actcaggtta tagttgcctc gaccgtcaaa accttttgga
    15901 ttgacgcctt tgaaatcccg aaccctcggt aaagccacat ttataaacct atctagaaat
    15961 tcaaacattt tttcaccacg aagagttaac atacaaccta tattcatgtt ttctcttagt
    16021 ttaaaacctg cgatagactt cttagctatt gtcttaactg ctttttgtcc agtaatagct
    16081 gcaagtgtat ccagtgcaga ttctaacatt tttgggttgg aatgaccttc acccattcca
    16141 acatttaata caattttttc aagcttagga acctgagaaa ctgacttaaa gttaaacttt
    16201 ttcatcagtg caggcttaat ttcttggtta tattttttct ttaatcttga ttgttccatg
    16261 attaaccttt tgctctaaga gttctttctt tcgaaccatt ctttacttca aaaccaactc
    16321 gagctccctt cttctttttg gagtcataga gcattacatt agaaatatga attgaatttt
    16381 ctatttcaat aatgcctcct tgcggatttt cctgtgttgg tcgaacgtat ttttttctct
    16441 tattgatacc acttacaaaa actctatcct ttcttttatc aaaggataaa acttttcctt
    16501 ttttaccttt atctgcacca gcaatacaga tgacttcatc atcttttttt agtttaattt
    16561 tcttaaactt agtaggttct gaacctctat aaggtaattt ggttgccata ttataatacc
    16621 tccggtgcta aagagataat cttcataaat ttcttatctc tgagctcacg tgcaactggt
    16681 ccaaagatac gagttccttt tggattgcct ttatcgtcga tgattgctac tgcattgtca
    16741 tcaaaacgaa tataacttcc atctggtctt ctgatctctt ttctggttct tacaacaaca
    16801 gctctttgaa ctgcttttgc atgcactttc tttccgattg aatcccttaa accataagct
    16861 ggttgagaat ctttaactgc tacaatgatt tcatccccaa tagttgcata acgtttcttg
    16921 gatccgccta gaaccttaac gcattgaact cttttcactc cagagttgtc tgctacctgt
    16981 aaaaaagttt gcatttgaat cattttattt cgctctctct acaattttta aaagtttgtg
    17041 tcttttttct ctggataatg gtcttgtttc cattgctaga ataacatcgc cttctttgca
    17101 ttcgccttgt tcatcatgaa ctttaatacg agatgttttg cgagtaatct ttttaaacat
    17161 tgggtgattt tgtctcacct caacttccat tacaatcgtt ttagccattt tagtgctaac
    17221 tactttgcct tgaattgttt tgcgcttttt tacttctact ttagtttcca taaatacaat
    17281 ccttatttct tcttagcagc tgcttttgct gcttcgatct ctctttctct ttgttcagtc
    17341 agaagtctcg caactttttt tcttaggttt cgtatgatca ttgggttttc caatgaacga
    17401 gcaaccacgt attgaaatct ttgtgttctc aattcttttc ttgcatcagc aagttgtgtt
    17461 ttgatttctg catcttttag gtctttcaat ttcagcttca tattaaattc ctcttaacga
    17521 attctgtgtg gattggaagt ttgtaagaag caagtgtaag agcttttttt gctgatgctt
    17581 catcaatacc actcatttca aataatactc ttcctggtcg aacttcagac acccagaatt
    17641 ctggatttcc ttttccttta cccattctag tttccgctgg ctttttagtt actggaacat
    17701 gtggaaatat acgaatccat aattttccac ctcttttcac tcttctgttg atagaaaccc
    17761 tagcagcttc aatttgtctt gcagtgattc taccggagct aacagccatt aaaccgtatt
    17821 ctccaaagga tactttatta gcaacttcgt ctttgccttt aagtcttcct atgtgccatt
    17881 ttctatattt tactctttta ggtgctaaca tttttgttac cagatcctat taattagttc
    17941 tgcgctttac cgcatattta tcttctactt catcgttaga tgtagagata tgatctccag
    18001 aataagtcca aactttaata ccaatttgtc caaaagttgt taaagcttct tttgttccta
    18061 agtctatctt tgctcttaaa gtgtgaagtg ggatacgtcc atctttgtag ctttctgttc
    18121 ttgccatatc agctccattt aaacgtccag agatagaaat tttaattcct tctactcccc
    18181 ctctcatagc tcttctgagt tctgctttca ttactcttct gaaaggcata cgttgctcaa
    18241 tttgagtagc aatggtttcc gcaatcgctt gcgctactgt ctcaggtttt ttcacttcaa
    18301 taatattgat attgattggc ttatcggaca ttggcttcaa taatgatttt acattttcaa
    18361 tgttttggcc tttttgtccg atcacaactc ctggcttagc tgtatgaaga ttcacattga
    18421 ttttttctgg gaatctttcg attacaactt ttacaagaga agagttttta aatttgtttt
    18481 gcaaaaactc tctgatttta atatcttcat gaagattttt tgtgtattct tcttttgcaa
    18541 accagataga atcccaagtt cttgtaattc cgactcttaa accgattgga ttaacttttt
    18601 gtcccattca atatcttcct attaatttga taatactact gtgatgtggc tcagtctctt
    18661 tctgattcga gcagcacgtc ctcttgctct tgctttaaat ctcttcatga taggtccatc
    18721 ttctacatag atttttttga tgtagacttg cgaaagatct atctttggat cagcaaccat
    18781 tgcattagcc gcagcagaat tcacaacgtt taagatttca tcagcgactc ttttttgggt
    18841 aaattttaaa atatctacag cttctgtgta gtcatagcct cttacttcat tcgctacgag
    18901 tcttgctttt cttgcagaaa ctcttacata ttttgctaca gctttagatt ccatttgaga
    18961 ctcctatttt ttaaccttct tgtctgaacc agaatgacca cggaatgttc tggtaggtgc
    19021 aaattcccct aacttatgtc cgaccatatt gtcgttgata tatacaggga taaatttgtt
    19081 tccattgtga atcatcacag tatgtccaat catatcagga aatattgtgc ttcttcttga
    19141 ccaagttttg aaaggttttt tatttccttc cgaattcatt tttgtaattt tgcgcattaa
    19201 gtggtcatcg ataaaaggac cttttttaag acttctagac ataaattagt ttcctctatt
    19261 cctatttctt tttctgcctt ggacgataaa tttatcgctt ggtcttgctt ttcttcttgt
    19321 tttataacct ttagtaggtt gtccccaagg gctcacagga tgtctacctc cggaagtttt
    19381 accttcccca ccaccatgtg ggtgatctac tgggttcatt acaacccctc tcaccttagg
    19441 tcttctacct aaccatctag atctacctgc ttttccgatc gaaactaaat tgtggtcttt
    19501 atttcctacg attccaattg ttgcatagca ttcttttcta actttgcgaa cttcagaaga
    19561 aggtaacttg agagttacat aatctccatc tttcgccgat acggttgcaa aagatccagc
    19621 tgttcttgcc atttgtccgc cttttccgcg gtgtagctcg atattgtgaa cgtcagttcc
    19681 aggaggaatc ttttcaagtg gaagagaatt tccaactttg atttctgcag attcgcctgc
    19741 tactaaaaca tcacctactt tcaaaccttc tggagcaatg atataagaga attctccatc
    19801 tttataagaa agaagtgcaa taaaagaagt tctgtatgga tcatattcaa ttgtttttac
    19861 aactgcaggg attccatatt tatttcttct aaagtcgatg attctatatt tacgttttac
    19921 tctcccacct ttgcgtctaa cggagatttt accaccatct cctctaccag cattgtattt
    19981 aacggttgta gtaagaggtt tatgtggtct ctcttctgtt atttcagcaa agtctaaaac
    20041 tgacttatag cgagttgccg ctgtgacagg tttaaatttt ctaataccca taattatact
    20101 cccttaccaa actctaggtt tgccccgttg tcaaaagtaa cgactgcttt tttccaatgt
    20161 ggtcttctag ctggaaaatt tctgaatttt ttcatttttc catgatacat gattacgtta
    20221 acagaactag gtgttacttg aaatattttt ttgatcgctt ctttgatcat tactttgttg
    20281 gcatctggat gaactttaaa agtgtatttt acagttcttt ttccgatatc ctttccaatc
    20341 tcttgcgcag attgagattt ttcagtgatg ataggagcaa tgattatact atctatattc
    20401 atttacttac atccttatac tggcttacga tttctttgag agcactttct gtaataatca
    20461 attgattatt atagaggata tctctacata ctattctctt tgcatttaca tatttaagag
    20521 ttggaatatt ttttgtggat gcttttagga atttatcttc gccagaaact accaggctaa
    20581 atgccccatt tgtaacaccc attgtcttga agatattgaa aatttctttt gtgctatatt
    20641 tagtcatagc aatatcttct aaaatattga ttttactttc ggaagctttt ttattgagaa
    20701 ttgaaactac tgccactctc ttaacgttac gagatagagg gatagaataa tctcttggtt
    20761 ttggtccatg aacagtaccg ccccctaccc aatgaggagc tctagaagaa ccttgtcttg
    20821 cacgaccagt tcctttttgt gaccaaggct ttttaccgcc ccctctcact tctcctctat
    20881 ctttagttgc atgatttccg gatcttagat ttgcattctc tgctttgata gcatcataga
    20941 tggcaccgct actaactttg ctagtaaaga tttcacctgg taattctact tctgcaagag
    21001 attgtccggt ttttgaatat ttcctggctt tcatataaat ccctttttcc ttttacaact
    21061 tttcaatcgt aatgatcgat ttttcaggac ctggaacaga accggatact aatagtatgt
    21121 tcttatcctc ataaaccttc actactctta aatttttgat agaagttttg atgcttccag
    21181 ttctacctgg gagctttact cctttaaata ctctagaagg tgtagagttc gcacccatag
    21241 aaccaggatg acgatggaat ctagatccat gcgcagcagg tccaccggag tgaccgtgtc
    21301 tttttaccac accctgatat cctttccctt tgctgatgcc agttaccttt actcgatcat
    21361 tgtctttaaa gatatctgcg attttgattt cagaaccagc agcgggagca tctccagtaa
    21421 gagcaaattc ttttaaatat ctcttaggag cgatgttgtt tttggcaagg tgagttactt
    21481 ctgctttaga aagctgagtt tctcttgcat tgccaaaggc taactgaact gccgagtatc
    21541 catctgattc tacagttttt acctgtgaaa caaaacatgg acccacctca atcacagtca
    21601 cgggaatcat gttcccattg tctgcatata tatgcgacat gcctaatttt ttaccaatta
    21661 aacccttagt cataattatt ccttaagact taatatctac cgagacacct gcagggagtt
    21721 gaagtttcat taatgcttct actgtatctt catttgtgtt taagatatca atcagtcttt
    21781 tatgtgttct catttcaaat tgctcacgtg attttttgtt cacgtgaggt gatcttaata
    21841 ttgtaaactt ctcaaccttt gttggaagtg gaataggacc tgaaacagtt gcgcctgttc
    21901 tttttgcagt cgcaacgatt tcatatgtag actgatccac taatttatga tcaaacgctc
    21961 taagtttaac tctaattctt tgtccagtca tactcttact caatgatctc agctacaacg
    22021 cctgagccta tagtctttcc accttcacgg atagcaaagt ttaatccttt gtccattgcg
    22081 attgggtgaa tcaattcgat agtcacagat acgttgtctc ctggcataac catttccatt
    22141 cctgccggta aaacacaaac ccctgtaatg tctgtggttc tgaaatagaa ctgaggacga
    22201 tagttgttaa agaatggttt atgacgacca ccttcatctt tggttaatac gtaaacctca
    22261 gcagtaaact ttttgtgagg agtaattgtt ccaggtttag caagaacttg tcctctttcg
    22321 atttcttctt ttttagttcc acggagcagt gcaccgatgt tatcccctgc ttctgcttga
    22381 tcgagtaatt tacggaacat ctcaatacca gtaacaactg ttttagcagt gtctttgata
    22441 ccaacgattt ctacttcttc gttgattttg agaactccag tttcaactct accagttgca
    22501 acagttccac gaccagtaat agagaatacg tcttctactg gcattaagaa tggtttgtca
    22561 gtaacacgtt ttggatttgg aacgtatgta tcaagagctt ccattaattt aagaacagct
    22621 tttgttccga gttcagaatc ttcaccttca agtgctttta aagcagaacc atggatgatt
    22681 gggatcggac cttgtccttc tttagggaag ttgtattttt tcaaaaggtc acgaatttct
    22741 tcttcaacca tttcaatcat ctcagctctt tcatcgtctt ttaactgatc tgctttgttt
    22801 aagaaaacaa cgatgtaagg aacgcctact tgacgagcga gaagaatatg ctcttttgtt
    22861 tgtggcattg caccgtcagt tgcagacaca actaaaatcg cagcgtccat ttgcgcagca
    22921 ccggtaatca tgttcttaac ataatccgcg tgacctggac aatcaacatg tgcatagtga
    22981 cgatttactg tttcatattc ctggtgagaa gtagcaattg taatacctct ttctctttct
    23041 tcaggtgcgt tatcaatctc gtcatattta acagctttgt tctttccacc gatcacttta
    23101 gcaagtgtag atgtaattgc cgctgtaagg gtagttttac cgtggtcaac gtgaccgatt
    23161 gttccaacgt ttaagtgtgg tttgcttcta tcaaattttt ccttagccat ctctaaataa
    23221 actcctaaaa atatttctca aaaaattata acattcaaaa ttcgaaatgt gacaacgagg
    23281 tgaatatagt tttttgagac aaaaaacctt ttccttttgt catatttctt aaagcacttg
    23341 tgaagccaag catgttttct gcagcagctt cacaaatcag taaagattta ttgccatgga
    23401 caatatctgt ctttactatt ttagcatttc tcttaccgag aacggctaaa actgaaccgt
    23461 aatacgattc aggaatgaca acttcaaatt gacacaatgg accaatttgg attgtgcatt
    23521 ctggtaagta agacttaata ccactaatga tggccacctt aagtaggttg gacgtaaagt
    23581 cgtttacaac ttctggtctc tcaaaagaga gaacccttag gtttactcca agaactggaa
    23641 aacctcgact tccattagat aatacttcgt aaaacgccga ttctacagag gacttataat
    23701 cctcctcaag ttcacattcc caaaggacag aacttgaaaa atcaggactt ttctcaaggg
    23761 aactaattag agtcccactc ttcaactttt gattaaaagc agagtgttcg aaaattaccg
    23821 ttattgccat gtttttaaat ttttcatatc tggcaacttt taaattccca aaagtaaaaa
    23881 tttctgaaat aaattctttt aatctttgaa tcgcaacatc taaatgcaac tcccctgctc
    23941 ccctcagctc aaattgaccg gtatccgctt tctctttgaa actaagcccg gaatcttccc
    24001 aaaccaattt ttctaagcct gaacgaagag tttctttttc ttcagactta ttcggttcca
    24061 acagaaggac atactcgttg ttatgcgatg aaactacatt tgcctcttct tttttttcga
    24121 gaaataatga ccccgttgat acatgagaag tttcagaaat agttgcggca aatatggaat
    24181 agccgtccat aatatctaat tttgtgattt cattgggagt gatttcataa aaagattcta
    24241 acttaaaact tttttctttg gaaaaataaa tatctccaat tcgcatcggc tcataggctt
    24301 tgataaaata aatttttcct aaatccggat gcaattcccg cttaaataaa acacctctac
    24361 aatctaaatc ttgcgaatga cgtctatgaa aatcagtttc acaaataaaa tcaagaagtt
    24421 cttttacact agctccatgg cttgcacttc cagcaaaaag cggagtgagt ttcgattctt
    24481 taaatcctga ataaagacct tcgagtaaaa cttttttaga aggtttctta aaatactttt
    24541 ctacaagaga atcattccat tcaataaagg aaagttcaat atcctcttgg atttcttttt
    24601 ttaaaaaagt atattcaatc tgatttgtaa atccatctgt aatggtaaaa agtggattta
    24661 gtttacgtgg caaagattca tttaattcta aaataaaatt ttttaaattc tctgccttat
    24721 caattttatt gataaaaata atgagaggaa ttccaagctc tgtaatttct tctaaaagca
    24781 tttctgtttg agagcgaatg cctgtcgtaa tatccaaaag taaaacagct atatcgacag
    24841 ccaaaagaga atttcgaact tgtgcataaa aatccaaatg tccgggtgta tcaattagat
    24901 tgatcgaata ctttttattt ttgtatttat aacgaatctg tatggtagtt gctacaatcg
    24961 aaattccacg ctcaatctct tctcgcattc gatcactttc tgttgttcct tccagcactg
    25021 aaccaggact atagatctct cctgcttcaa ataaaattcg ctcagtcagt gtcgtctttc
    25081 cagaatcgac atgagcaaag attcctatat ttcgaatatt gcgttgcatg gtagttcaat
    25141 gtcattttta tacaataggt ttttaggaaa agtaggaatg agagagaggt tttaaatcgc
    25201 ttgaggagta atacaaatac cgtaatttat ttttgaggtc tataaaataa gagctataga
    25261 gagaattgtg gatataacag aaacgagaat ggttacatta gagttgccac ccgaaattaa
    25321 accgggaagg cacaaattgg tgattgtaat ctacgaagag ccattggatt caga
//
LOCUS       DAOQOS010000080        24924 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_939_length_24924_cov_5.954763, whole genome shotgun sequence.
ACCESSION   DAOQOS010000080 DAOQOS010000000
VERSION     DAOQOS010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 24924)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24924)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24924
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_939_length_24924_cov_5.954763"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(247..2898)
                     /locus_tag="PLF66_16605"
     CDS             complement(247..2898)
                     /locus_tag="PLF66_16605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008592757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HMZ65714.1"
                     /translation="MSGIKPPSTKKKMDLSPRTRAFSPYGFLFFSIFCFFSMLSFQDV
                     DAQQSANLFGRLGHYFAYLTFYLFGRASYILGVLGFIFGIISFQKKEPDVGNLLLSVP
                     IILLACSIFCSIFEVNITSLKDGGGYIGLKFSTALEYIFGQSGRIILNVVIFLYGALI
                     IVRESPREINKDYLLNLWKETISKYPIREKLESYIHQVRKKEEEETEEELTGKIPWFE
                     IRRREQSYVDELETPISTVEEEITEESPVFNQTSAPKGFKTTNSEIIDKFEYKSVISE
                     LKPKTTQAIYKEEIKIDPIPKFKSTPSQIISKINQPSLPNEQITASNLQSYISSTSEK
                     KAVDPEDFEEEIMEAELVEADEMLDSSLPGLMTDEDEVLADDLQDMPTEEVDLFGNKI
                     ENKPAQKPVRKQSTVPDIIPKRNTDYYLSSRILARKTEKKSDPLFKLEAEKIGLHIQE
                     IIKQYGYDSKVVSYQRGPIITRYELTPPQGVKLGRITSLTDELKLNLAVKSIRMVAPI
                     PGKNTIGIEVPNKYRDDVYLGDILKDTSALQTQRDLNIVIGKDSITGENVSIDLNKLP
                     HLLVAGTTGSGKSVSMNSMICSLIFNKSPDDVRFVMIDPKMVELALYEDIPHLLMPVI
                     TDPRKATKALAWAVQEMEIRYNLVSELKCRDLKTYNEKVATSLKFSRGRYKHLPYIVI
                     FIDELSDLMMVSGKELEDYITRISQKSRAVGIHLVMATQRPSVDVITGLIKANCPARM
                     AFHVAQKTDSKIILDYNGADALLGKGDFLYKSPTSPDLQRIQAPFISEDEIEQIVEEV
                     RKHADPEYVDINLDEESFQEESSEEDEESFEEAWMIVKTDRKASASYLQRRLRIGYNK
                     AARLMEMMEERGYVGPQIGSKPREILR"
     gene            complement(3377..4924)
                     /locus_tag="PLF66_16610"
     CDS             complement(3377..4924)
                     /locus_tag="PLF66_16610"
                     /EC_number="1.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HMZ65715.1"
                     /translation="MEIINWISENPFWSIGIALFIFLILLFIRDVSQTRHTIIHNFPV
                     VGHLRYFLEMIGPELRQYWVANDKEERPFDRTERRWIYATAKGENSNFGFGTTELLYD
                     PGYPIIKHKAFPFPESKVYKISGDPTCIPSLKVIGDFHKRKYPYRPYSIINISAMSFG
                     SLSKNAIMALNVGAKEAGAYHNTGEGGISPYHLNGADVVWQIGTGYFGARTKEGRFDI
                     GILKQKVAANKTVRMIEVKLSQGAKPGKGGILPGIKVTAEIAKAREVEIGKDCISPGS
                     HAEFSTVPELIQFIELIAGETGLPVGIKSAVGEIEFWELLALEMKKTKRGPDFITIDG
                     GEGGTGAAPLTYADHVSLPFKIGFQRVYTLFQKHGISERVVWIGSGKLGFPDRAVVAL
                     AMGCDMVNVAREAMLSIGCIQAEKCHTDHCPAGVATQNWWLQKGLNPTLKSKRAALYL
                     KGFRKELLALAHSCGYEHPAQFTGRDIEISLGMNRFTTLEDLLGYKRDERKFTRMADY
                     GSFPSRS"
     gene            complement(4984..5994)
                     /locus_tag="PLF66_16615"
     CDS             complement(4984..5994)
                     /locus_tag="PLF66_16615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015682669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HMZ65716.1"
                     /translation="MKAIQLQGNFGLENLKLADLDEPSVLYPNDVLVRIHAASLNYRD
                     YLVVTGKYNPKFKIPFVPVSDGAGEVLQIGNAVTEFKVGDRVLFQFAPKWLDGDANYE
                     QARYTLGGPLDGTLMEKKVYPESALVKIPDHMSYEEGATLPCAALTSWSSLVTYGRIF
                     AGDTIVVLGTGGVSIFALQIGKILGAKVIVTSGSDEKLEQAKSLGADYTINYKTFPEW
                     GKKVKEITGEGADHIIEVGGAGTLEQSIRAIRPFGQISLIGILAGGAKDLNLLPVLMN
                     NVKMQGILVGSKAGLAAMCKAFTYHKIKPVIDKTFPLEESVKAIEYLKSGSHFGKIVI
                     SL"
     gene            complement(6025..6924)
                     /gene="xerD"
                     /locus_tag="PLF66_16620"
     CDS             complement(6025..6924)
                     /gene="xerD"
                     /locus_tag="PLF66_16620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010569547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HMZ65717.1"
                     /translation="MASSAQVKLLQNFQEYLSVEKGLSDNSIFSYGYDLNKFKSYLDK
                     QQIDLLNVQSKDIMKFLVEEKSRKISAKTLAREVVAIRQFYKYLRDEKQVDFNPTDKI
                     ETPEVMRVIPDHLTLEEINELFDHIKEDNIFELRDKCIFELLYSSGLRISEACNLKME
                     DVDLENMTLSIDGKGGKSRLVPFGEKALNILRLYLEKSRPEILKHRKCEYIFVSKKGS
                     FINRKSVWRLLNLYIKRTGIQKKVTPHTLRHSFATHLLENHADLRSVQELLGHIDIST
                     TQIYTQMANKSLKEVHKKHHPRG"
     gene            7146..7778
                     /locus_tag="PLF66_16625"
     CDS             7146..7778
                     /locus_tag="PLF66_16625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65718.1"
                     /translation="MQSKRSNKKNSLRKKNLTNDKPSFFSYFILTTLFFTFLSCSREK
                     QISQDAYKKEQAGKKAEALYDYTRALEINPDYAFANKRIGFILSESPESQDVAIHHLE
                     LAKKESESDIEIDLKLFDSYLLNGEFSKLKKLRGELSVSLDLQTFQLLDDIYNCILKK
                     EESKNTAKKIQISTDVPYAKHFYRSWAACYLFAELPEKSEEIKNRYRNAE"
     gene            7768..8943
                     /locus_tag="PLF66_16630"
     CDS             7768..8943
                     /locus_tag="PLF66_16630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65719.1"
                     /translation="MLSKLILYIFLIFFFSEGLLWIVRPSALEFYRIQKAYHKLDPEY
                     LIDLEPNVNVHVKHFQNFFQMDFSTNEMGFRGSPKVNNDLPQIVCIGDSVTMGFGVSD
                     KETFCNQLDGYTTPSGIKYQALNLGVDAYGPSHINLKLEKFLPKLNTKVLYYFPSNGD
                     NIDEVTFYSKKKNPLALRLFQIQFQLAKYSYLFLALKMTQENLIYRFNETFIWPLKKI
                     DKTKKCLDSILPADECSDAYFPYTPYNLKDDFIRSPKRNPNEAPRFSEKECSDTPVPF
                     EIPNGMIEQINQMVNFTKDKNIKLVLFLAPIDIETAYCSQKDKFHHYYAYLSTLKEYL
                     IQKNIDFVDLNEYTKQMTFEGQMNVRPYYIFGDGHYTALGNAWVYQIVKKKTEEILK"
     gene            8944..10353
                     /locus_tag="PLF66_16635"
     CDS             8944..10353
                     /locus_tag="PLF66_16635"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HMZ65720.1"
                     /translation="MLFNSLHFYIFCFIVFPIYFLLQEEKQKKLLLWASVYFYACLKV
                     AFVPLLLLSFIATFLGSIRIFESDSMKTKKLWLYFVLLINLGILIFFKYTDFLRSISH
                     DFQVFIGGNPGEFKAIGFILPLGISFYTFQAIAYAVDVYRGILKPERSFSEFSLFLLF
                     FPQLVAGPIMRADVLIPQFKGRKIFSLENFSIGMGQIALGIFKKTMIADPISDFIEPI
                     FATPSNYDYLSCLLGVYFFTIQIYCDFAGYSDIAIGSGKILGFNIPINFKRPYLASSS
                     TDLWRRWHISLSTWLRDYIYITLGGSRVSILRNYFNVFVTMVIGGIWHGAAWTFVIWG
                     FICGVSLTIEKFFIEFGYEKYFLKIPKLLRISYTFSLFMLGALFFRSLNFELGIQMLK
                     RILTLESSGIFTINWILFIPISILFIIEVLEETEHLSKIKESGFYKLVRVPMLASIFL
                     VAGAIYTVTASPQFYYFQF"
     gene            10364..11302
                     /gene="trxB"
                     /locus_tag="PLF66_16640"
     CDS             10364..11302
                     /gene="trxB"
                     /locus_tag="PLF66_16640"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000940582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HMZ65721.1"
                     /translation="MAHKIVIIGSGPAGHTAAIYAARANLNPIMYEGFMAGGVAAGGQ
                     LTTTTEVENFPGFPEGIDGTKLTQLFREQSAKYGTTIITQTVTKVDFSKRPFKIYADD
                     EICEAEAVIIATGATAKRMNIKGEHDYWQRGISACAVCDGALPIFRNKELAVIGGGDS
                     AVEEATHLTKFGSKVYLIHRRDKLRASQVMQNRAMNHPKIEILWNTTVEEAIGGAGGI
                     SAIALKDTVNGSTKTLPVGGLFYAIGHTPNTEIFKDFLELDDVGYIKTVPGTTKTNIE
                     GVFAAGDVQDRVYRQAITAAGSGCMAALEAERFLMH"
     gene            complement(11536..14676)
                     /locus_tag="PLF66_16645"
     CDS             complement(11536..14676)
                     /locus_tag="PLF66_16645"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HMZ65722.1"
                     /translation="MGILELANKVFFNFSTVGSLIPALFFLLTAGFLLRTENKTKATF
                     HLGIVYLLFSLVPLDYFINFAVYDPRSAYTRWISAVISLPASFHLIQFFLYFPENKKF
                     RFSSFLLVFGWIFTLLVWGYYIYATYQSPIIYNFSGHYWDFDADPAQEYIALVILLAV
                     VFFILTALWKIYHSNGKDVWVLAGLLLSAMVITVIPAIANFLSRRGLIERGTYFAYFA
                     LLVMIGTFSLIVIYINNKRDKSSFMTKIIGISLVTFFLVLQGVGYFSIRDKEVTFDEL
                     KRKDVALTLHQVIIPEDLRYVKYYSLEKDYFISHANSPETNFDMTQMKIEMINSFYYD
                     SLVKLESTEKLKESDEKLVAILSEMPVYLEAYKVSIVEEIKKAREENSDFEIESYLSN
                     LEHTLSKKRRDIQGLKDENFKTNLITYLEKQKDSTFQNFKKSINSYLERSKSEGRELK
                     NEVLWFISILRPSGSRFYRTDLKTGQHFVSYFYSDLKFKRMYEVGFDYLSFRRFVHST
                     SVRYTLVILTVVCIVLFGFRLFLYGALLKPLDSMIAGVREVNKGDYNVTIPIAVSDEL
                     GFLGRSFNRMVRSIRGAKYKLQKHAQELEEKVSERTSELKTTLGEIQELKTHQDGDYF
                     LTSLLIKPLSKNNNKSDRVHVDFIVSQKKKFQFRSYKEEIGGDICVSYNIILRNRPMT
                     VFLNADAMGKSMQGAGGALVLGSVFQSIIQRTKYSHEMQEEYPEKWLKNTFVELHKVF
                     ESFKGSMLVSILMGLIDDNTGYMYYVNAEHPYTILYRDGKAGFIETSSLLRKLGTIDM
                     QSSVFVQTFQLKPGDVILSGSDGKDDVFLGVNEEGIRVINEEESWILDKVEKAQGDLK
                     KIYSEITRNGEVTDDLSLVRVEYDSHIADFVIDLDMEEVDDLMLEAKQYTSKKGNKMA
                     IEILERAYKKSKAHIELNRKLINLYLHAGNFRKAAKTAEVFSEIHPEEYEYIYIASFC
                     YKRAALFDKALETAERLKLRFPDMAKNLSNLAEINFYLKDYQRSLEMVRRALKQDPEN
                     IRALMIKKKLAKLNIK"
     gene            14953..15924
                     /locus_tag="PLF66_16650"
     CDS             14953..15924
                     /locus_tag="PLF66_16650"
                     /inference="COORDINATES: protein motif:HMM:TIGR02136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HMZ65723.1"
                     /translation="MKKNTFYTMIILSALLVGFCGKKTNRVTADGSSTVYPITSAAAE
                     EFGKQGNGVQVTVGISGTGGGFKKFCNNETDISNASRYVKEEEINACASKKIEFIELP
                     IGYDGLSIVINKENDWAKELTVEDLKKIYQKEGYVKTWKELNPKWPDLEIKAFAPGQD
                     SGTFDYFAEEIVKKKDNFRQQGVSFSEDDNTIVTGVAGNKGGIGFFGVAYYESNKDKL
                     GLAKVVNPKTNEAVEPTFENVKSNKYAPLSRPIFIYVKAASASVESVDKFINFYLDNA
                     AKFTKETGYIPFPDEIYAKVKKRYADKVKGTVVKSKEDKHKPIAELY"
     gene            15973..16947
                     /gene="pstC"
                     /locus_tag="PLF66_16655"
     CDS             15973..16947
                     /gene="pstC"
                     /locus_tag="PLF66_16655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002770331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HMZ65724.1"
                     /translation="MADNNPKSINLLTQAVTSGDRSKIFSRYGKRPSYSLKEKLIRYI
                     LRSFSWVTIGVTLFIIGILFVDAIELFRRYSLIDFITGTEWEPFGESKKLGILPLVSG
                     TLMIAFGASMISLPLGFGTAVYLTQYAGKEVDRFMTPIIEILSGIPTVVFGYFALFAV
                     TPFLQKFFQNMELFNATSASIVVGISCIPLVSSISSEALRAVPPSIRNGAYAVGMNKF
                     NVVTKVIVPSAISGIIASFILAFARAVGETMAVTLAAGASPNMEWNYFKGIQTMTAFI
                     VQISLGDTPAGSTEYYTIYAVGLMLFLITFAFNLIATMIIKRYREIYK"
     gene            16950..17831
                     /gene="pstA"
                     /locus_tag="PLF66_16660"
     CDS             16950..17831
                     /gene="pstA"
                     /locus_tag="PLF66_16660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002770334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HMZ65725.1"
                     /translation="MDKEFNKTRFKYAVLGIAFQTFCLAANFSVIIFLVLLLGDVFLQ
                     GYSTLNLNFLTSYPSRFADKAGILASIVGTFYMMLLTALISVPLGIGAAIYLEEYTRE
                     TRFIRFVKLNIQNLAGVPSIIYGILGLTLFVRFMDLGRSLIAASLTMALLILPIITIA
                     SQEALRAVPQSFRLAGYGLGLTRWQVIRYQVLPMVATGMSTGVILALSRAIGESAPLV
                     MVGALTYVAFLPSGLLDSFSVLSIQIYNWSSFPQQEFHALAASAIIVLLVMLFILNVI
                     AILLRIYFQNKIRGMNR"
     gene            17845..18642
                     /gene="pstB"
                     /locus_tag="PLF66_16665"
     CDS             17845..18642
                     /gene="pstB"
                     /locus_tag="PLF66_16665"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017789372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HMZ65726.1"
                     /translation="MSPKFTVNENNSDNTKSTISIHCENVDIYYGDFPAITDLNLDIE
                     ESKITALIGPSGCGKSTFLRSLNRMNDFIENNRVTGKILLKGQDIYDPHIDPVEIRLR
                     VGMVFQTPNPFPKSIYENIALGPRLNGYTGDLNELVETALQKAYLWEEVKDKLKQNAY
                     SLSGGQQQRLCVARAIAMQPEVLLMDEPTASLDPISTSKIEDLIVELRKDYTVIIVTH
                     NLQQAARIGDTTCLFYHGKLIETGSTKDMFNNPKEKLTKDYLSGRFG"
     gene            18657..19328
                     /locus_tag="PLF66_16670"
     CDS             18657..19328
                     /locus_tag="PLF66_16670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65727.1"
                     /translation="MPKVFICDIEKIEKNFEDVKSRFPEINRDIEVPRDEFNDFVASN
                     MVLAYKRLNELLIADINIFSNKHVMEMLELNHIVLCGHDEKLRYEYMHHLEATRVRFF
                     EGLPPVKRWFNKHDDKDGVAKVAAEIYVGVLSQPQLFIEGNHRTGSLIASWILLRGGL
                     PPFVLTAENARAYFNPSSQIKFSDKRVISDKFKLPKYQKSFKTFLESNIKKKFLTEAE
                     FNKQK"
     gene            19414..19785
                     /locus_tag="PLF66_16675"
     CDS             19414..19785
                     /locus_tag="PLF66_16675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HMZ65728.1"
                     /translation="MRSLKKFFSILLVFIFLQCGTTFSIYETWSYEKRKENRNGSPMF
                     GIPSDFPPVYAGLKIDYYTFQFFKNNYFLGFFVLLDFPLSFTFDTILLPITIPVAISH
                     CQKDKQEKYLHPYACKEGELW"
     gene            19829..20260
                     /locus_tag="PLF66_16680"
     CDS             19829..20260
                     /locus_tag="PLF66_16680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65729.1"
                     /translation="MKHIFLITIFFFLTVGCGVKGKPVEYKDACNLENNDTYIEVSGY
                     LNYGGSVYCSSTSGRMDCGFDFLENTNSQKGFSVNIEQGSSSNSVEKFESGYKKEDIK
                     IRDDKGKIISLSDKVTVTGKIRTVKEPDVVCYIKVYKIEVK"
     gene            20910..22202
                     /locus_tag="PLF66_16685"
     CDS             20910..22202
                     /locus_tag="PLF66_16685"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HMZ65730.1"
                     /translation="MNLIDLENKQSILPDFNPDKKPIFSAYDCGKLALTAQKLIQENK
                     FIFLNEKMRFQTLYEEYSENPELDFLPVFDSNTNEIIGYTMRQKILAELSKGPFSKEL
                     LLRPEVIVENIYDKRVLCIDAYTNLSEASYILMQREEDIRFDPFIITLDGYFYGICSI
                     RKVLDGLNRFLEMDMSFCEISQLKLNLESNYNFSKQSSILSNYQINCLHGPGGDYVGT
                     FSLNENLSLFIHLDVCGKGLKASNVLMVLGTIFKTWIEMETYIDRRYFKLSDKLFHLN
                     HLAYRLTPDEMYATGVFILHDKLTQTIEVADFGHGFVWLKRGSKSARLTDFIPKEDEM
                     EKIQQMPFFGINENFIVKTAKFKVKQGDLLFVCTDGIIEQKNIYKEEFGINRIEKVLS
                     TYLGKNPKELNDQILEEWNDFRKHHKVTDDYSVLSICV"
     gene            complement(22429..24132)
                     /locus_tag="PLF66_16690"
     CDS             complement(22429..24132)
                     /locus_tag="PLF66_16690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65731.1"
                     /translation="MKKIVIILFLFIFTCIGQKGAKIKSIFSLSNTTLSNSKDLRSFQ
                     FLQISPPVTATISGNQITAIIPSSISVKNLVATLDTTGNSILANGVLQVNGQTPNDFT
                     NPIVYSIVAADGTKSDFTVTVNNTGYFILGSAGNINITGLSASDSALEMYYDTSGNSF
                     KSFSNNTMIGAILPANTQYALRFKSQPTGRVCSLTGGSLSGTLTADVSFSINCVTGYL
                     VGGKVLAVLSTFTIPANGATVTTVAGSFPPTASAGSSDGVGSAARFSFVLGITSDGKD
                     LYVGDAGNHCIRKYEIATANVTTIAGLCGTSGTTDGTGTFARFNFPMQIASDGTNLFI
                     CDRANNMIRKVVISTGVVTTLIGSGGASGDQEGIGSVARINFPNGITTDGNYIYVSDR
                     GNNKIKRMLLSSEIVSTIAGNGIAATVDSANGLTASLDTPCDSVSVGTSLYFVDCFGH
                     TLRKIELSGSFAVTTLAGSGAAATTDGTGLAASFNNPHGIETDGSNLFITEYNGQYIR
                     RVGISSLNVVTIAGGVGYQDGTGASALLGNLGGISTDGNRLYFVDSSNNAIRRIDNPP
                     D"
     gene            24313..>24924
                     /locus_tag="PLF66_16695"
     CDS             24313..>24924
                     /locus_tag="PLF66_16695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65732.1"
                     /translation="MILPIPNKTTLNNLKLHIYSSFQEVNAEHWDLIIQNKNIYSTIP
                     YLSAIEKSLVDQIQFRYFIYYNSKLYPVAVGVVQFLNFIDKGFDDKEEVCRIRNSIKK
                     KLLTEKGIQMMSCGTPFASGETGFFYSDTLTHKEAYKHLSHALLQIQKKEKSTPVILI
                     KELFPESIEKRNILVQHKFNEFEIDVNMILKISPKWKNLNDYMD"
BASE COUNT         8101 a         4515 c         4157 g         8151 t
ORIGIN      
        1 attttttatt aaaatctatt ctgtaaaccc tttttcttat ttcgcaacag actgagaatt
       61 catattgcac cgctctttga aagaatataa tttcagtcaa aatgaaagca agattttttt
      121 ggaatgggat ttccccgtag gcgagcctcc gaaggctagc ctatggggaa ggtcaatgaa
      181 aaacggtcaa taaatacctc aaaaattcaa atcgatctaa tttttttcta agaatttaaa
      241 tcgcaactaa cgcaaaattt ctcttggttt agagccaatc tgtggtccta catagcctct
      301 ttcttccatc atttccatga gacgtgctgc tttattgtag ccaatacgaa ggcggcgttg
      361 taaataactg gcacttgctt tacgatctgt ttttacaatc atccaagctt cttcaaatga
      421 ctcttcatct tcttcactcg attcttcttg gaaggattcc tcatctagat tgatgtctac
      481 atattctggg tctgcgtgtt tacgaacttc ttctacgatt tgttcaatct catcttctga
      541 aataaagggt gcttgaatcc tttgtagatc cggagaagtt ggagacttat ataagaagtc
      601 tccttttccg agtaacgcat ctgctccatt gtaatctaag ataatttttg aatctgtctt
      661 ttgtgctaca tgaaaagcca tacgggcagg acagtttgct ttaataagac ctgtaatcac
      721 atcgactgac ggacgttgtg tcgccatcac taaatgaatt ccgactgctc tcgatttctg
      781 gctgatacgt gtaatataat cttctaactc ttttccagaa accatcatca agtcagataa
      841 ctcatctata aaaataacaa tatacggaag atgcttgtat ctacctctag agaatttgag
      901 agaagtagcc accttttcat tatatgtttt taaatctcta cattttaatt cagaaactaa
      961 attatagcga atctccattt cttgcacagc ccaagcaagt gctttggttg cttttcttgg
     1021 atccgtaatc actggcatca gtagatgcgg aatatcctca tataaagcaa gctctaccat
     1081 ctttggatcg atcatcacaa atcgaacgtc atccggcgac ttattaaaga tgagtgaaca
     1141 aatcatagaa ttcatagaaa cagattttcc agaaccagtt gttcccgcca caaggagatg
     1201 aggaagtttg tttagatcaa tcgagacatt ctctccagtt atactatctt ttccaattac
     1261 gatatttaaa tctctttggg tttgtaatgc actcgtgtct tttaagatat cacctaagta
     1321 tacatcatct ctatatttat ttggaacttc aataccgatt gtattttttc ctggaatcgg
     1381 ggctaccata cgaatgcttt ttactgcaag attgagtttt aattcatctg ttaaagacgt
     1441 tattctacct agctttacac cttgtggggg agtcaattcg tagcgagtga tgattggtcc
     1501 tctttggtaa gatacaacct ttgagtcata gccatattgt tttataattt cttggatgtg
     1561 tagaccaatc ttttcagcct ctaatttaaa aagagggtct gatttttttt ctgtcttcct
     1621 agccaaaatc cgagaagata aataataatc tgtatttcgt tttggaatta tatctggaac
     1681 agtcgattgc tttcggactg gcttttgtgc aggtttattt tcaattttat ttccaaacaa
     1741 atctacttct tctgttggca tatcttgcaa gtcatccgct aaaacttcat cctcatcagt
     1801 cataagccca gggagagagg aatcgagcat ttcatctgct tccactaatt ctgcttccat
     1861 aatttcttct tcaaagtctt ctgggtctac tgcttttttc tcagaagtgg aggagatgta
     1921 ggactgtagg ttactagctg ttatctgctc attcggaaga cttggttgat tgattttact
     1981 aatgatttga cttggagttg atttaaattt tggaatcgga tcaatcttaa tttcttcttt
     2041 gtaaatcgct tgggttgttt tcggtttcaa ttctgatatg acagatttat actcaaactt
     2101 atcaatgatt tcagagttcg ttgttttaaa tccttttggc gcacttgttt gattaaaaac
     2161 gggagattct tctgtaatct cttcttccac agtgctaatc ggagtttcca actcatctac
     2221 atagctctgc tctcttcttc gaatttcaaa ccatggaatt tttccagtga gttcttcttc
     2281 ggtttcttcc tcttcttttt ttcgaacctg atgaatataa gattctaatt tttctcgaat
     2341 tggatattta gaaatcgttt ctttccagag atttagcaga taatccttgt ttatctctct
     2401 tggagactcc cgaacaatga taagagcacc atacaaaaag attacaacat ttaatataat
     2461 tcttcccgat tgaccaaaga tatattcaag ggcagtggag aattttaaac caatataacc
     2521 accaccgtct tttaggcttg tgatattgac ttcaaaaatg ctgcaaaaaa tggaacaagc
     2581 gagaagtata atcggaacgg atagaagtag gtttcctaca tctggctctt ttttctgaaa
     2641 actgataatt ccaaatataa aaccaagaac tcctagaata taagaagccc ttccaaataa
     2701 ataaaatgta agataagcaa aataatgtcc aagtctacca aatagattcg cagattgttg
     2761 tgcatctacg tcttggaacg aaagcataga aaaaaaacaa aatatgctaa aaaacaaaaa
     2821 tccataggga ctaaatgccc tcgttctagg acttaaatcc atttttttct ttgtggatgg
     2881 gggtttgata cctgacatag ttatacggat ttattatcgg cagagtaaaa aaatctcaat
     2941 gcgtaaaaat ctcgaaaatt ttttcaatca aataccataa attgccattt acccaagaaa
     3001 gtataccttt gaggctttga tttcgaaagg aatcccagtt gaagtcctat ttctttttcc
     3061 taggattgtg attcgggaaa cgtttttgtt atattaacgg ttcgatgtaa cagaaaaatt
     3121 taaagattac cactgatgag gaggatgagc accgataaaa gatacgataa tttaaaatta
     3181 tcaatcgttt ctataccgaa cgccaaaatt aatttccctt ttttgagtca cagataagtg
     3241 ttcggtctat gccaaatgct actcaaataa aatccttgtg ggaagtttct tttgtcattt
     3301 ggtatatatc gttcagttgt cggtgacctc cggtggtaat tcttaagttg gcagcattgg
     3361 catacgtggg atcgtttcac gatctagaag gaaaactacc gtaatccgcc atacgagtaa
     3421 attttctttc gtctctttta taaccgagta aatcttctaa agtagtaaag cgattcattc
     3481 cgagagaaat ttcaatgtct cttcccgtaa attgagccgg gtgttcataa ccacaagaat
     3541 gagctaacgc aagtaactct tttcgaaaac cttttaagta aagtgcagcg cgttttgatt
     3601 tgagtgtagg atttaaacct ttttgcaacc accagttctg tgttgcaaca cctgcaggac
     3661 aatgatctgt atgacatttt tctgcttgaa tacaaccaat cgaaagcatt gcttctcttg
     3721 caacattgac catatcacaa cccatagcta gagcgactac cgcacgatct ggaaaaccca
     3781 gtttacctga acctatccaa accactcttt ctgaaattcc atgtttttga aagagagtat
     3841 agactctttg aaatcctatt ttaaaaggaa gagaaacatg gtctgcataa gtaagaggag
     3901 cagcaccagt ccccccttct cctccatcga ttgtaataaa atcaggtccc ctcttggttt
     3961 ttttcatttc aagagctagt aattcccaaa attcaatttc gcctaccgca gatttaattc
     4021 caacaggaag tccagtctca cctgcgatta gttctataaa ttgaatgagt tctggaacag
     4081 tagaaaattc agcatgagac cctggagaaa tacaatcctt tccaatttct acttctcttg
     4141 cttttgcaat ctcagcggtc actttgattc ctggaagaat tccacctttc cctggctttg
     4201 ctccttgcga aagttttacc tcaatcattc gaacagtttt atttgcggca actttttgtt
     4261 tgagaattcc aatgtcaaac cgaccctctt ttgttctcgc cccaaagtat cctgttccta
     4321 tttgccaaac gacatccgct ccatttaaat gataaggaga aattccacct tctcctgtat
     4381 tgtgataggc tcctgcttct tttgctccga cgttaagcgc cataattgca tttttactga
     4441 gagatccaaa agacatcgca gaaatattga ttatcgaata aggtctatat ggatattttc
     4501 ttttatgaaa atcgccaatt actttgagac ttggaataca ggttggatcc ccggaaattt
     4561 tatacacctt agattcagga aaaggaaatg ctttgtgttt tataattggg tatccaggat
     4621 catacaataa ctcagtcgtt ccaaatccaa aattgctgtt ttcccctttt gccgttgcgt
     4681 aaatccaacg cctctctgtt cgatcaaatg gtctttcttc tttgtcattt gcaacccagt
     4741 attgcctgag ttccggtcca atcatttcta agaaataacg aagatgacca accactggaa
     4801 aattatgaat gatcgtatgc ctagtttgtg atacatcccg aataaataaa agaattaaaa
     4861 aaatgaataa ggcaatgcct attgaccaga aaggattctc agatatccaa ttgattattt
     4921 ccataacaaa tctccaaatt aaaaatcaaa ttgaatcaaa gagtaaaaaa actgcaacca
     4981 gttttagagg ctaattacaa ttttaccgaa atgacttccg gattttaaat attcaatggc
     5041 tttcacagat tcttctagtg gaaaggtttt atcaatgact ggttttatct tatgataggt
     5101 aaatgcctta cacattgctg caagtcctgc tttgcttcca acaaggatac cttgcatctt
     5161 cacattattc ataagaacag gaagtaaatt cagatccttt gctcctccgg ctaaaattcc
     5221 aatcaagcta atttgcccaa aaggacgaat ggctcgaatc gattgttcta aagttccagc
     5281 accgcctact tcaatgatgt gatctgcccc ttcccctgtg atctctttta ctttctttcc
     5341 ccattccgga aatgttttat aattgattgt ataatctgct ccgagtgatt tagcttgttc
     5401 taatttttca tcacttccag aagtaacaat gaccttagca ccaaggatct ttccaatttg
     5461 taaagcgaag atagaaaccc ctcctgttcc taagacaaca attgtgtcgc ccgcaaaaat
     5521 tcttccatag gtaacaagag aagaccaaga agtaagggcc gcacaaggaa gagttgctcc
     5581 ttcttcataa ctcatgtggt ctggaatttt tacaagagca gattcaggat agactttttt
     5641 ttccatgaga gttccatcaa gtggtccccc tagcgtataa cgtgcttgtt cgtaatttgc
     5701 atctccatct aaccacttgg gtgcaaattg aaatagaact cgatctccta ctttaaattc
     5761 tgtaacagca tttccaattt gtaatacttc tccagctcca tcactaacag gaacaaaagg
     5821 aattttaaac tttggattgt atttaccagt tacaactaaa tagtctctat aattgagaga
     5881 ggctgcatgg attcttacta agacatcatt tggataaaga acagagggct cgtcaagatc
     5941 tgcgagcttt aaattttcta acccaaagtt tccctgcagt tgaatcgctt tcatgatgag
     6001 aaacttttct gcggctaagt agaattatcc tctaggatgg tgtttcttat gcacttcttt
     6061 gagagattta tttgccatct gtgtataaat ctgtgttgta gaaatatcga tatggccaag
     6121 taactcttga acagatctta gatctgcatg attttctaaa agatgtgtag caaaagaatg
     6181 tctgagtgta tgaggagtaa ctttcttttg aatcccagtg cgcttgatat aaagatttaa
     6241 taaacgccaa accgatttgc ggttaataaa agaacctttt ttagaaacaa aaatgtattc
     6301 acattttcta tgttttaaaa tttcaggacg gcttttttct aaatagagtc tgagtatatt
     6361 taatgctttc tctccgaaag gaactaatct ggatttgccg ccttttccat caatacttag
     6421 agtcatattt tctaagtcca catcttccat ttttaaattg caagcttctg aaatacgcag
     6481 tccagaagaa tacaaaagtt caaatataca tttatctctt aattcaaaaa tattgtcttc
     6541 tttgatatga tcaaatagtt cgttgatttc ttcgagtgtc aaatgatctg gaatcactcg
     6601 catcacttca ggagtttcta ttttatcagt agggttgaaa tctacttgtt tttcgtctcg
     6661 taagtatttg taaaattggc gaatcgcaac tacttcgcga gcgagcgttt tcgcagaaat
     6721 ctttctgctt ttttcttcta ctaagaattt cataatgtcc ttagattgaa catttagtaa
     6781 atctatttgt tgtttatcta aataagattt aaatttattt agatcatatc catacgagaa
     6841 aatggaatta tcgctaagac ctttctctac tgaaagatat tcttggaagt tttgtagtaa
     6901 ttttacttga gcactgctag ccaatttttt ttctcactct acttgattaa taagaaaatc
     6961 ggtcgaaact cttttctttt tttgattttt ttcttaaaga ggataagttt ttttataatc
     7021 cactttactg tactagacaa aaatacagat aaaatcatta catttagaaa aaagttttaa
     7081 agattttcgg gataaatatt ccttgcatac taggttcaaa aattcaattt tatataagac
     7141 ataatatgca atcaaaaaga agcaataaaa aaaactctct ccgcaaaaaa aacttaacaa
     7201 atgataagcc ttcatttttt tcctatttca ttctcaccac tctctttttt acttttctgt
     7261 cctgctctag agaaaaacag atcagtcaag atgcttacaa aaaagaacag gctggaaaaa
     7321 aagcagaagc actttatgat tatacacgtg cactcgaaat caatccagac tatgcatttg
     7381 caaacaaacg gattggtttt attttatcag aatcacccga gtctcaggat gtagcaattc
     7441 accacctaga gcttgccaaa aaagagtctg aatctgatat agaaattgat ctaaaactat
     7501 ttgattcata cttattaaac ggagaatttt caaaactaaa gaaactaagg ggagaactta
     7561 gtgtttcatt ggatctacaa acctttcaac ttctagatga tatatataat tgtattttaa
     7621 aaaaagagga atctaaaaat actgcaaaga aaatacaaat ttctactgat gttccctatg
     7681 caaagcactt ctacagaagt tgggcagctt gttatttatt tgccgaactt ccagaaaaat
     7741 cggaagaaat aaaaaataga tatagaaatg ctgagtaaat taattcttta tatttttcta
     7801 atattctttt tctcggaagg tcttttatgg attgtaagac cttctgcact cgaattttac
     7861 cgcatacaaa aagcctatca taaacttgat ccagagtatt taatcgattt agaacctaat
     7921 gtgaatgtcc atgtaaagca ttttcaaaat ttttttcaaa tggatttctc aacaaatgaa
     7981 atgggattta gaggttcacc aaaggttaac aatgatttgc cgcagattgt ttgtattgga
     8041 gattctgtca caatgggatt tggtgtaagt gataaagaaa cgttttgcaa tcagttagat
     8101 gggtatacaa ctcccagtgg gatcaaatac caagcattga atttaggtgt agatgcatat
     8161 ggtccctctc atatcaattt aaaattagaa aaatttcttc caaaactcaa tacaaaagtt
     8221 ctttattact ttcctagcaa tggggataat attgacgaag tgacttttta cagtaaaaag
     8281 aagaatcctc tcgcgctaag attatttcaa attcaattcc aattagcaaa gtatagctat
     8341 ttatttttag ctcttaaaat gacacaagaa aatctaatct atcgcttcaa tgaaacattt
     8401 atctggcctt tgaaaaaaat agataagaca aaaaaatgtt tagattcaat ccttccagca
     8461 gatgaatgca gtgatgctta cttcccatat actccctata atctaaaaga tgatttcata
     8521 cgatccccaa aacgaaatcc aaatgaagct ccgagatttt cagaaaagga atgttctgat
     8581 acgccagttc cctttgaaat tccgaatgga atgatagagc aaatcaatca gatggtaaat
     8641 ttcacaaaag ataaaaatat taaattagtt ctttttctag cgcctatcga cattgaaacg
     8701 gcttactgct ctcaaaagga taaattccat cattactatg catatctctc tactctgaag
     8761 gaatatttaa ttcaaaaaaa cattgatttt gttgatctaa atgaatacac aaagcagatg
     8821 acgtttgaag gacaaatgaa tgttcgcccc tactatatct ttggagatgg gcattatacg
     8881 gctcttggaa atgcttgggt ctaccaaatc gtaaagaaaa aaacagagga gattttaaag
     8941 tagatgttat ttaattctct tcatttttat attttttgtt ttatcgtttt tccgatttat
     9001 tttcttctac aagaagaaaa acaaaagaaa ctacttcttt gggcatctgt ttatttctat
     9061 gcttgtttaa aagttgcctt tgttccacta cttcttcttt cctttattgc aacctttctt
     9121 ggatccattc gtatttttga atctgattca atgaagacaa aaaaactatg gctctatttt
     9181 gttttgctaa tcaatttagg aatcttaatc ttttttaaat acacagactt cttacgctcg
     9241 atatctcatg acttccaagt ctttattggg ggaaatcctg gcgagtttaa agcgattgga
     9301 tttatacttc ctcttggaat ttccttttat actttccagg cgattgccta tgcagttgac
     9361 gtctatcgtg gaattttaaa accagaacgg agcttttctg aattttctct ctttctgctt
     9421 ttctttcctc agttagttgc aggaccgatt atgcgagcag atgttttaat tcctcaattc
     9481 aaaggaagaa agattttctc actcgaaaac ttttccattg gtatgggaca gattgctctt
     9541 gggattttca aaaagacaat gatcgcagat ccgatttcag attttattga accaattttt
     9601 gctacaccgt ctaactatga ttatctctct tgcctactgg gtgtttactt ttttacaatt
     9661 caaatctatt gtgatttcgc aggctattcc gacattgcaa ttggttctgg aaaaatttta
     9721 ggatttaata taccaatcaa ctttaaaaga ccttatctgg caagctcatc tactgatcta
     9781 tggagaagat ggcacatatc gctttctact tggcttagag attatatcta tatcacttta
     9841 ggtggaagta gagtttcaat tcttagaaat tatttcaatg tatttgtcac aatggtaatc
     9901 ggtggaatct ggcatggagc ggcttggact ttcgtaattt ggggatttat ctgtggtgta
     9961 agtcttacaa tcgaaaagtt ttttatagaa tttggctatg aaaaatactt tttaaaaatt
    10021 ccgaaactgc ttcgaattag ttatacattc tctcttttta tgctaggagc tttgtttttt
    10081 agatccctca attttgagct aggaattcaa atgctgaaaa gaatcttaac gctagaatct
    10141 tctggaattt ttacgatcaa ttggattctt tttattccaa tttccatttt atttatcatt
    10201 gaagttctag aagaaacaga acatttgtct aagataaagg aatctggatt ctacaaacta
    10261 gtccgagttc ctatgcttgc ttctattttt ttagtggcag gcgcaattta cactgtgact
    10321 gcaagtcctc agttttatta ttttcaattt taggagttta taaatggcac ataaaatcgt
    10381 aatcataggt tcaggacctg ctggtcatac agcggcaatc tatgcggcaa gagcaaatct
    10441 aaacccaatc atgtatgaag gtttcatggc aggtggggtg gcagcaggtg gacaactaac
    10501 gaccaccaca gaagtcgaaa attttccagg ctttccagaa ggaattgacg gaaccaaact
    10561 gacccaacta tttagagagc agtcggctaa atatggaacc actatcatta cacagacggt
    10621 tacgaaagtt gattttagca aaagaccttt taagatttac gctgatgatg aaatctgtga
    10681 agcagaagca gtaatcattg cgacaggcgc aacagcaaag agaatgaata taaaagggga
    10741 gcacgattac tggcaaagag gaatttctgc ctgtgcagtt tgtgatggag cacttcctat
    10801 ctttagaaat aaagaacttg ccgtgattgg tggaggagat tcagcagtag aagaagcaac
    10861 tcatctcaca aagtttggat ccaaagtata cctaattcac agaagagaca aactcagagc
    10921 ctcacaagtt atgcaaaaca gagcgatgaa tcatcctaaa atcgaaattt tatggaatac
    10981 aacggtggaa gaagcgatcg gtggtgctgg tggaatcagc gcgatagcac tcaaagatac
    11041 agtcaatgga tccacaaaga ctcttccagt aggtggactc ttttatgcga taggtcatac
    11101 accgaataca gaaattttta aagacttctt agaattagat gatgtaggct atatcaaaac
    11161 agttccagga actacaaaaa caaatattga aggtgttttt gcagcaggag acgtgcaaga
    11221 tagagtctat cgtcaagcaa tcacagcagc tggctccggc tgtatggcag ccctagaagc
    11281 cgaaagattt ttaatgcatt gatgtaagta gctactcaaa ggagaaaaca gattaccaag
    11341 gatgaacacg gatagacacg gataaaataa taattattaa aaccatcctt gtttatctct
    11401 gttaatcctg tccttcgctc caactattcg caaccaactc catcacattt ggtgaaaaat
    11461 ccttaacttg acagcataga ccatcggtgt tcatccgtgt gcatccgtgg ttaaaattct
    11521 tttattgaac ggacgttact ttatattcag cttcgctaat ttctttttaa tcataagtgc
    11581 tcgaatgttt tcggggtctt gttttaaagc acgacgaacc atttcaagag agcgctgata
    11641 atcctttaaa taaaaattta tttcggctag attgcttagg tttttggcca tatcgggaaa
    11701 tctaagcttt agtctttccg cagtttctaa agccttatca aataacgcag cccttttata
    11761 acaaaaagaa gcgatataaa tatattcata ttcttctgga tgaatttctg aaaatacttc
    11821 tgcggtcttt gctgcttttc taaaattacc agcatgtaaa tagagattaa tgagttttcg
    11881 attgagttca atatgagctt tacttttttt gtatgctctt tccaagattt caatcgccat
    11941 cttattccct tttttagaag tatattgctt ggcttctaac attagatcat caacctcttc
    12001 catatctaaa tcaattacga aatcggctat atgggaatca tactcaactc taacaagaga
    12061 caaatcatcc gtaacctcac cattccttgt aatctcggaa tatattttct ttagatctcc
    12121 ttgggctttt tctactttat ctagtatcca tgattcctct tcattaatga ctcgaattcc
    12181 ctcttcattc actccaagga aaacatcatc ctttccatca gaaccagaga gtataacgtc
    12241 tcctggcttc aattgaaagg tttgcacaaa tacagaactc tgcatgtcta tcgtcccaag
    12301 tttacgaagt aaactacttg tttcaataaa cccagctttt ccatctctat ataagattgt
    12361 atatggatgc tctgcattca cataatacat ataccctgta ttgtcatcta tgagccccat
    12421 tagaatggat actaacatag aacccttaaa actttcgaat accttatgta attctacaaa
    12481 ggtatttttg agccattttt ctggatattc ctcctgcatt tcatggctgt attttgtgcg
    12541 ttgtatgata gactgaaaga ctgaaccgag taccagtgct ccccctgctc cttgcatcga
    12601 ttttcccatt gcgtctgcat ttaaaaaaac agtcatagga cgattgcgca aaataatatt
    12661 ataagaaaca caaatgtctc cacctatttc ttctttataa gatctaaact gaaatttctt
    12721 tttttgtgag acaataaaat caacatgcac tcgatcactt ttattattgt ttttactaag
    12781 tggtttaatc aaaagagaag ttaaaaaata atctccatcc tgatgtgttt ttagttcttg
    12841 aatttcacca agagtagttt ttaactcaga agttctttca cttacctttt cctctaattc
    12901 ttgagcatgt ttttgtaact tatattttgc tcctcgaata gaacgaacca ttcgattaaa
    12961 tgatcttccc aaaaaaccaa gctcatcaga aactgcgatt ggaattgtta cattataatc
    13021 ccctttattg acttctcgaa cacctgcgat catagagtcg agtggtttta ggagtgcccc
    13081 atataaaaat aatctaaatc caaataaaac aatacagaca accgtaagga taacaagcgt
    13141 ataccgcaca gatgtagaat gaacaaatct tctaaaagat aaataatcaa atcctacttc
    13201 atacattctc ttgaatttta aatctgaata aaaataggac acaaaatgct gacctgtttt
    13261 caaatcggtt ctgtagaagc gagatccaga cggacgtaag atggagataa accagaggac
    13321 ttcatttttt aactctcgcc cttccgattt gcttctttct aaataggagt ttatgctctt
    13381 tttaaaattt tgaaaggtag aatccttttg tttctctaga taggtgatta aatttgtttt
    13441 aaaattttca tcttttagac cttgaatatc tcttcttttc ttggatagcg tatgttctag
    13501 attcgagaga taactttcaa tttcaaaatc tgaattttct tctcttgctt tttttatttc
    13561 ttcaacaatc gataccttat atgcttccaa atacactggc atttcactga gtattgctac
    13621 caatttttca tctgactctt tgagtttctc tgttgattct aattttacaa gagaatcata
    13681 ataaaaagaa ttaatcattt caattttcat ttgagtcata tcaaaatttg tctcaggtga
    13741 atttgcatga gaaataaaat aatctttttc caggctataa tatttcacat atcgcaaatc
    13801 ttcagggatg atcacttggt gtaatgttag agctacatct tttctcttta actcatcaaa
    13861 ggtgacttcc ttatcccgaa tcgaaaaata accaactcct tgtaaaacta gaaaaaaagt
    13921 tacaagacta atccctataa ttttagtcat aaaggatgac ttatctcttt tattgttgat
    13981 atagatcaca ataagagaaa aagtcccaat cattacaaga agagcgaagt aagcaaaata
    14041 ggttcctctc tcgattagac ctcttctact taaaaaattt gcgatagccg gtatgactgt
    14101 tattaccatc gcagacaaaa gtaaaccggc aagaacccaa acatcttttc catttgaatg
    14161 ataaattttc cagagagcgg ttaaaataaa aaaaacgact gcaagtaaaa tgactagagc
    14221 tatatactct tgtgccggat ctgcatcaaa atcccaataa tgcccagaaa aattataaat
    14281 aattggagat tggtaagtgg catatatata gtaaccccaa acgagaagtg taaaaatcca
    14341 tccaaaaact aaaaggaagc tagagaatct aaatttttta ttttcaggaa aatagagaaa
    14401 aaactgaatc aaatgaaaag aagcaggaag agaaatgaca gcactgatcc aacgagtgta
    14461 tgcagacctt ggatcgtaga ctgcaaagtt gataaaataa tcaagaggaa ctagggaaaa
    14521 gagtaaataa acaattccca aatgaaatgt agccttagtc ttattttcag tgcgcaataa
    14581 aaaaccggca gtgagcaaga aaaatagagc tggaatcaga gaacctactg ttgaaaaatt
    14641 aaaaaaaacc ttatttgcaa gctctagtat tcccataaaa ttaggggcta aaattatttg
    14701 ctttctagaa taatgcaagc aaataattct gtttatacaa acctaggtta gaatttttat
    14761 tacaggtttg aataagattc tatttcaaat gactcacatt gtacttgctg catgtcctag
    14821 atttaatttt cctgtcatat atatgtaaca aaaaaaatat aatcagaaag gtgaatagaa
    14881 aaggaaagtt tggtaggcaa taacaagcca aaactgaatt ctattcagga atctaaaaca
    14941 aaaaggttat ttatgaaaaa aaatacgttc tatacaatga tcatcctttc ggctttacta
    15001 gtgggttttt gtggaaaaaa aaccaatcga gtaacagcag acggatcaag cacagtttat
    15061 cctatcacgt cagctgcagc tgaagagttt ggaaaacaag ggaatggagt tcaagtcaca
    15121 gttggaattt ctggaactgg tggtggattt aaaaaattct gtaacaatga aacagatatt
    15181 tccaatgctt ctagatatgt aaaagaagag gaaatcaatg catgtgcctc caaaaaaatt
    15241 gagtttattg aactaccaat cggttatgat ggactatcta ttgttatcaa taaagagaat
    15301 gactgggcaa aagagctcac agtagaggat cttaagaaaa tctaccaaaa agaaggctat
    15361 gttaaaactt ggaaagagct gaatccaaaa tggccggatt tagaaatcaa agcattcgca
    15421 cctggacaag attctggaac ctttgattat tttgcagaag agattgttaa gaaaaaagac
    15481 aatttccgtc aacaaggggt tagcttcagc gaagatgata acacgattgt aactggtgta
    15541 gctggaaaca aaggtggaat aggatttttt ggagttgctt attatgaaag caataaagac
    15601 aaattaggtc ttgctaaagt agtaaatcca aaaacaaatg aagcagtgga accaacattt
    15661 gaaaatgtaa aatccaataa atatgcacca ctctctcgac ctatattcat ttatgtaaaa
    15721 gcagcatctg cgtctgtaga atctgttgat aaattcataa atttctatct agacaatgca
    15781 gctaaattta caaaagaaac tggatacatt ccatttcctg atgaaatcta tgcaaaagtg
    15841 aaaaaaagat atgcagataa agtaaaagga actgttgtta aatctaaaga agataaacac
    15901 aaaccgatag cagaacttta ctaattagtt tcatcgcgtg ggggaaaccc cacgttaaaa
    15961 ggaaaatcga atatggcaga caataatcca aagtcaatca acctgcttac acaagcagtg
    16021 acttccgggg accgctctaa aattttttcc aggtatggaa aaagaccctc ctatagctta
    16081 aaagaaaaac tgattcgata catactaaga tctttctcat gggttacgat aggcgtaact
    16141 ctttttatta taggcatcct atttgtagat gcaatcgaac tttttagaag atatagtctt
    16201 atagatttta tcacaggaac tgaatgggaa cctttcggag aatccaagaa acttggaatt
    16261 ttaccacttg tttcaggaac tcttatgatt gcctttggag cgtcaatgat cagcctgcct
    16321 cttggttttg gaactgctgt ttatctaact caatatgcag gaaaagaagt agaccgcttt
    16381 atgactccga ttatagaaat cctttctgga attccgacgg ttgtattcgg ttattttgca
    16441 ctctttgcag taacaccttt tttacaaaaa ttctttcaga atatggagtt atttaatgca
    16501 acctctgcgt ctatcgtcgt tggaatttct tgcattcctt tggtttcttc catttcatcg
    16561 gaggctcttc gggcagttcc tccatcgatt cgaaatggag cctatgctgt tggaatgaac
    16621 aaatttaatg ttgtcaccaa ggtaattgtt ccttctgcga tatccggaat cattgcctcg
    16681 tttattcttg cctttgctcg tgcagtaggc gaaacaatgg cagtcacact tgctgcaggt
    16741 gcttctccta acatggaatg gaattatttc aaaggaattc aaaccatgac agcctttatt
    16801 gttcaaatca gtttgggaga cactccagct ggttctacag aatattatac aatctatgca
    16861 gtcggtttga tgttatttct catcacattt gcgtttaatt taatagctac catgatcatc
    16921 aaacgctatc gggagattta taaataatta tggataagga atttaacaaa accagattca
    16981 aatatgcagt tcttggaatt gcatttcaaa ccttttgttt agctgccaat ttttctgtta
    17041 tcatattttt agttttatta ctgggagatg tttttttaca ggggtattct actctcaatc
    17101 tgaatttctt aaccagttac ccatctcgct ttgctgataa agctggaatt cttgcgtcta
    17161 tcgttggaac cttttatatg atgcttctaa cagctctaat atctgttcca cttggaattg
    17221 gagctgctat ttatctagaa gaatatacaa gagaaactcg ttttattcgt tttgtaaaat
    17281 taaacattca aaacttggca ggcgttccat caatcatcta tggtatttta gggctaacat
    17341 tatttgtccg ctttatggat ttaggtagaa gtttaatcgc tgcttctctt acaatggcac
    17401 ttcttatttt acctattatc acaatcgctt cacaagaagc tttgcgagct gtcccacaat
    17461 cttttcgact ggctgggtat ggtctaggac ttacaagatg gcaagtaatt cgttaccaag
    17521 tattgccgat ggttgcaacg ggaatgtcaa ctggagtcat tcttgccttg tcaagagcca
    17581 ttggggaatc agctcctctc gtaatggtag gggcacttac atatgttgcc tttttgccct
    17641 ctggtttatt agatagtttt agtgtcttgt ccatacaaat ctacaactgg tctagctttc
    17701 cacaacaaga gtttcatgcg ttagccgcat ccgctatcat tgttttacta gttatgcttt
    17761 ttattctaaa tgtcatagcc atcttactaa gaatttactt tcaaaataaa attagaggta
    17821 tgaaccgata aggtcgaaat cattatgagt cctaaattta cagtcaatga aaacaattca
    17881 gataacacga agtcaaccat cagcattcat tgtgaaaatg tagacatata ttatggagat
    17941 tttcctgcaa tcacagattt aaacttagat atagaagaat ccaagatcac agcactcatt
    18001 ggtccttcag gatgtgggaa aagcacattt ttaagaagtc tcaatcgaat gaatgacttc
    18061 attgaaaaca atcgagttac aggaaaaata cttttaaaag gtcaagacat ctatgatcca
    18121 catattgatc ctgttgaaat tcgactcaga gttggaatgg tatttcaaac tcccaatccc
    18181 ttcccaaaat caatttatga aaatattgcg ttaggtccaa ggcttaacgg ctatactggt
    18241 gatcttaatg agcttgtaga gactgcttta caaaaagctt atctttggga agaagtaaaa
    18301 gacaaactaa aacaaaacgc ctactcatta tccggtggtc aacagcaaag actttgtgtc
    18361 gctagagcga ttgcaatgca gccagaagtg ttactcatgg atgaaccaac agcttctcta
    18421 gacccaattt ctacatcaaa aattgaagac ttaattgtgg agctaagaaa agattatact
    18481 gttatcattg taacgcataa cttacagcaa gcggctcgta ttggagatac cacctgcctt
    18541 ttctatcatg gtaagctaat agagacaggt tccacaaagg atatgtttaa taatccaaag
    18601 gaaaagttaa cgaaggatta tctaagcggt cgtttcggtt aatacaggtt agccacttgc
    18661 ctaaagtatt tatttgtgac atcgaaaaaa tcgaaaaaaa ttttgaagat gtcaaatcac
    18721 gcttccctga aatcaaccga gacattgaag tccctcggga tgaattcaat gactttgttg
    18781 catccaatat ggttctcgca tacaaaagat taaatgagct actcattgca gatataaata
    18841 ttttttccaa taagcatgta atggaaatgc tagaactaaa tcatatagtg ctctgtggtc
    18901 atgacgagaa attgagatat gaatatatgc atcatttaga ggctactagg gttcggtttt
    18961 ttgaaggact tcctcctgtt aaaagatggt ttaataaaca tgatgataag gatggagtag
    19021 ccaaagtagc tgctgagatt tatgtaggag tattaagtca gccgcaactt tttatcgaag
    19081 gaaaccacag aacaggttct cttattgcaa gttggatact ccttcgtggt ggtctccccc
    19141 ctttcgttct tactgcagaa aatgcaagag cttattttaa tccttcctct caaattaaat
    19201 tcagtgataa aagagtaatc agtgacaaat ttaaattacc taaatatcaa aaaagtttta
    19261 agacattttt agaatctaat ataaagaaaa agtttctcac agaagccgaa tttaataaac
    19321 aaaaatagca acgaatagat gtagtaatgc tttttagatt ctttgccaaa aaaatgattt
    19381 ctgtttgtat tctttaaaaa caataaaaaa actatgcgat ctttaaaaaa atttttttca
    19441 atccttcttg tatttatttt tcttcaatgt ggaactacat ttagtattta tgaaacttgg
    19501 agttatgaaa aaagaaagga aaatcgaaat ggatcaccaa tgttcggaat tccatcggac
    19561 tttcctcctg tatatgcagg gttaaaaata gattattata catttcaatt ctttaaaaat
    19621 aattactttc ttggattttt tgtattactc gattttcctc tttcctttac ttttgataca
    19681 attctacttc ctatcacaat tccagtagca atatcccatt gccaaaaaga caaacaggaa
    19741 aaatatcttc atccctatgc ttgtaaggaa ggagaattat ggtagaaaaa atacgtatca
    19801 ttttttttga taactcattg gagtaattat gaagcatatt tttttaatca cgatcttttt
    19861 ctttcttacc gttggttgcg gggtaaaagg caaacctgtt gaatacaaag atgcatgcaa
    19921 tcttgaaaac aatgatacat atatcgaagt aagtggctat ttaaattatg gtgggtctgt
    19981 ttattgttcg tctactagtg gacgaatgga ttgcggattt gactttctgg agaatactaa
    20041 ttctcaaaaa ggcttttctg taaacattga acaaggctcc tcttctaatt ctgttgaaaa
    20101 atttgaatcc ggttacaaaa aagaagatat aaaaatacgc gatgacaaag gtaaaatcat
    20161 ttctctatct gataaagtta cagtgactgg aaaaattaga accgtaaaag aaccggacgt
    20221 tgtgtgctat ataaaagtat ataaaattga agtcaaataa tcatttccat aatgattaga
    20281 aacacatttt acaattccta taccaaacgc tagaagaaaa gtcctcatat aaaataaaga
    20341 tgcgtttggt taaatacttc tgaatgattc ctcaccttcc agttttccaa ctacgattca
    20401 aataattttt accaaggaaa actaccttcc cctttctgtc ctttggttta gattatagag
    20461 tcaacaataa gcagtattta aagtgaattc gacctaagaa gatgtttcct tttggcaccc
    20521 ccgaattcca cccgatgcta ttcgcatcca gcctgcgtag ggtggaattt cgggaatgac
    20581 ggtctgaaca atttatcaat tccttatacc gaactcatgt tttttaaccg aagatgcagt
    20641 aaatttaaaa taccttctac ctctattatt ataaaataat gacaataaac ttatatagat
    20701 ccaagtagaa gaaataaaag ctgggttgtt attcttttag aattcatcca aacttatttt
    20761 attcgattca aactattctg aatttgtaga gattagattc tttttcctat gtaaaatttt
    20821 ttttaagatt tttaaaatta cccttaaaaa ttacaatttt gtattctctc tgtaaatact
    20881 aagaaatata aatctattat tttcttacta tgaatttaat agatctagaa aacaagcaaa
    20941 gcattctccc tgatttcaac ccagataaaa aacctatatt ttctgcatat gactgcggca
    21001 aattagcttt aaccgctcag aaattaatac aagaaaataa attcattttt ttaaatgaaa
    21061 aaatgcgttt tcaaactttg tatgaagagt attcagaaaa tcctgagtta gacttcttac
    21121 ctgtatttga ttccaataca aatgaaatca ttggatatac gatgaggcaa aaaattctag
    21181 cagaactcag caaaggtcct ttttcaaaag aattactact tagaccagaa gtgattgtag
    21241 aaaatattta tgataaaaga gtgctttgta ttgatgccta tacaaattta tcagaagctt
    21301 cttatatttt aatgcaaaga gaggaagaca ttcgatttga tccatttatc ataacgcttg
    21361 atggatattt ctatggaatt tgttctattc gaaaagttct agatggactc aatcgttttc
    21421 tggaaatgga tatgtcattt tgcgaaatct cacaattaaa actcaatcta gaatcaaatt
    21481 ataatttctc aaaacagtcc agtattcttt caaattatca aatcaattgt ttacatggac
    21541 cgggtggaga ttatgtcgga actttttcac tcaatgaaaa tctatctctt tttattcatc
    21601 tagatgtttg tggcaaaggt ttgaaggctt ccaatgtttt gatggtatta ggaacgattt
    21661 ttaaaacttg gattgaaatg gagacttata tcgatagacg ctactttaaa ctctctgata
    21721 agctttttca tctaaaccat ttagcttata gattgactcc cgacgaaatg tatgcaactg
    21781 gagttttcat actccatgat aaactaacgc aaacaattga agttgctgac tttggacatg
    21841 gatttgtttg gctaaaacgt ggttctaaat cagcgcggct tacagatttt atccctaaag
    21901 aggatgaaat ggaaaaaatt caacagatgc ctttctttgg aatcaatgaa aattttatag
    21961 taaaaacagc aaagtttaaa gtaaaacagg gtgacctact ctttgtatgc acggatggaa
    22021 ttatagaaca aaaaaatatt tacaaagaag aattcggaat caatcgtatt gaaaaagttc
    22081 taagtacata tctaggaaaa aatccaaagg aattgaatga tcaaattcta gaagaatgga
    22141 atgattttag aaaacatcat aaagtaacgg atgactattc cgttctttct atttgtgtat
    22201 agacttaata ctatttatca tttttggtga ttgttaaaaa atgtcgccat aattcactca
    22261 aaaaaattcc taggaatcga gaagattgag aggataagaa ttagtaaatt cataccttag
    22321 attcatctct atctccattg attctatttt actctgttgt gatagaccca tctctaaaat
    22381 aaatagctct ttaagttttt ggtttttgta aagaatcaat ttagattttt agtcgggtgg
    22441 attatcaatt ctacgaatag cattattact tgaatccaca aaatagagac gattaccgtc
    22501 agtcgaaatc cctcctagat ttccgagaag tgcagatgcc cctgttccat cttggtatcc
    22561 gacccctcct gcaatcgtaa ctacatttaa actagaaatt cctacacgtc gaatgtattg
    22621 accattgtat tcggtgataa ataggttcga tccatccgtt tcaattccat gaggattatt
    22681 gaaactagcc gcaagacccg ttccatctgt ggttgcggca gccccacttc cagcaagcgt
    22741 tgtaacagca aagcttccag acaattctat tttacgaaga gtatgaccaa agcaatctac
    22801 aaaataaaga gaggtcccga cggaaacaga atcgcaaggt gtgtctaaac ttgcggtaag
    22861 accatttgca ctatctaccg ttgctgcaat tccattgcct gcaatggttg aaacaatttc
    22921 ggaagacaaa agcattcgtt tgatcttatt atttcctcta tcactcacat agatataatt
    22981 tccatctgta gtgattccat ttggaaaatt aattctagca acagatccta ttccttcttg
    23041 atcgcctgac gcaccaccag atcctatcag agtagtaacg actcctgtgc taatgactac
    23101 ctttcgaatc atattattgg ctctatcgca aatgaaaaga tttgtcccat cactagcgat
    23161 ctgcatagga aagttgaatc gagcgaaagt tccagttcca tcagttgttc cagaagttcc
    23221 acaaagtcca gcgatcgtag taacatttgc agtagcaatt tcatatttac gaatacaatg
    23281 atttcctgca tctcctacat ataaatcctt accgtcagac gtaataccca atacaaaact
    23341 aaatctagca gcactcccta ctccatccga agaaccggca gaagctgtag gaggaaaaga
    23401 accagcaaca gtcgtaacag ttgctccatt ggctggaatt gtaaaagttg ataatacagc
    23461 tagaactttt ccaccaacca aataacctgt tacacagttt atagaaaagc tcacgtctgc
    23521 agttagagtt ccagatagag agcctcctgt caaagagcat actcttccag ttggttgaga
    23581 tttgaatctt agcgcatact gtgtatttgc cggaagaata gcacctatca ttgtattatt
    23641 actaaaagat ttaaaagaat ttccacttgt atcataatac atttccaatg cagaatcaga
    23701 ggcacttaag ccggtaatat ttatatttcc tgcactaccc aaaataaaat atccagtgtt
    23761 gtttactgtt acggtgaaat cagatttagt tccatctgct gctacaatag agtatacaat
    23821 tggatttgta aaatcatttg gggtttggcc atttacttga agaaccccat tcgctaaaat
    23881 agaattccct gtagtatcta aggttgctac taaatttttt actgatatac tagaggggat
    23941 aattgctgtt atctgattgc cagatattgt tgcagtaact ggaggggaaa tttgtaaaaa
    24001 ctgaaagctt cttaaatctt tagaattaga taaagttgtg ttagataaag aaaagatcga
    24061 ttttattttg gctccttttt gtcctatgca agtaaaaata aataaaaaga ggattataac
    24121 gatttttttc atataagaaa tttttctttc atttggcata gacttaaaat cagattaaat
    24181 gaattttaga ttttgtcaac aaaggagctt ttgttttaga tagaaaccta ctaataaaaa
    24241 atttataaaa attttcttta cttttagaat caatagcaaa acctgaatgc tatcctctca
    24301 atccattcca aaatgatttt acccatacca aataagacga ctctcaataa tttaaaatta
    24361 cacatctatt cctcctttca agaagttaac gcagaacatt gggatttaat cattcaaaac
    24421 aagaatatct attcaactat tccatattta tctgcaattg aaaaatcgtt agtagatcag
    24481 attcaattta gatactttat ttattataat tctaaattat atcctgtcgc agttggggta
    24541 gttcaatttc tgaatttcat tgataaaggt tttgatgata aagaagaagt ttgtaggatt
    24601 cgaaattcca ttaaaaaaaa attactgaca gaaaaaggga ttcaaatgat gtcttgtgga
    24661 actccgttcg cgagtggcga gactggtttt ttttattcag acactctaac tcataaagag
    24721 gcttataagc atttatccca cgcactcctc cagattcaaa aaaaagaaaa atccacgcct
    24781 gtcattttaa tcaaagaatt atttcctgaa tcgatagaga aaagaaatat tttagtccag
    24841 cataaattca acgaattcga aatcgatgta aatatgatct taaagatttc tccaaaatgg
    24901 aagaatttaa atgattatat ggat
//
LOCUS       DAOQOS010000081        24621 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_959_length_24621_cov_6.401856, whole genome shotgun sequence.
ACCESSION   DAOQOS010000081 DAOQOS010000000
VERSION     DAOQOS010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 24621)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24621)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24621
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_959_length_24621_cov_6.401856"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            252..392
                     /locus_tag="PLF66_16700"
     CDS             252..392
                     /locus_tag="PLF66_16700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65733.1"
                     /translation="MKRTKKEIDNILEILAKPILFITGVNDYAVTKGFWIKVGKLPLD
                     ER"
     gene            889..1596
                     /locus_tag="PLF66_16705"
     CDS             889..1596
                     /locus_tag="PLF66_16705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65734.1"
                     /translation="MKVKMFYFILILGLVLETSLFAEVDLNYNYDYEMGGVKTKFSEW
                     VPYKLHKWNPKFLEDYYELYGLKLHYGEEELRKDIYFLKIGLESRFRHPNNSLCRVKN
                     EREYHKYRLLVSMHMNLQIMRAYMRIASQYDKRHVYFQNLDYAHDLKKSFKIAEGFYK
                     EAIPYWKKAEELAKKANEIKDEIDLGTMESERFEISNGKLNFGNIINDHLSRLAKKQE
                     TVEEYLKQNPEQDKPPL"
     gene            complement(1806..2240)
                     /locus_tag="PLF66_16710"
     CDS             complement(1806..2240)
                     /locus_tag="PLF66_16710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020778000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3805 domain-containing protein"
                     /protein_id="HMZ65735.1"
                     /translation="MSEIHYQPYRSPNGWYILAYPEHWEVEIIEEIPAFFDPDGAGAL
                     QVSAFENKLGSYDLSEELERFLSVHKISYDEERVATFTNNLGSQIKACEFIADERFWL
                     VYMISSGSKLIVCTYNSDETPDRELSVIITNIISSIQFLNHE"
     gene            2292..3455
                     /locus_tag="PLF66_16715"
     CDS             2292..3455
                     /locus_tag="PLF66_16715"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HMZ65736.1"
                     /translation="MNQKKSNSKKFSFPTMMVLLILLSIVGGFVLFLLSVILLIAFLF
                     YPIYKRYFLRKYPVQDIADEVSYAVTEDGWNLAIHRHKPMKPLPNAYPVIVVHGIITN
                     KYFMDLDVDHSLAYHLKLCGYDVFAVSLRGCGESHKKSEDQNFNFDDMVMKDVPAIIK
                     EVSNITGKNKINWIAHSMGAMIMYSYLGVAPKKEKDKVKSFISLGGPGNLSHSSPVLG
                     KAGVKYISLAKKIDLKLMAKFILPFVSIFNSPLKEIFYNPQVTTKNTVKKMLFGIENI
                     ADGLMLQTLEWMANGGDIISIDKKFNYTELQTKITCPILFIAGGYDNVATPKSLKSVY
                     NKVSSKYKEFHVVSESAGFSGDYGHACLVMGQNAKKEIFPLLEAFLLKHGKSK"
     gene            3777..6605
                     /locus_tag="PLF66_16720"
     CDS             3777..6605
                     /locus_tag="PLF66_16720"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HMZ65737.1"
                     /translation="MNQIKLKIYIFIQILFLLPVSAQETTQTNKVIIGKIQPYESETN
                     QNLEKEIQSTLASKLQESKFLISNSTESTLPSRLKEAKMANARYLLEGFYNKKSNDSN
                     LNLYIQIYDPQTERMIDAYSITDEVFQSEGLQLDKEELKENDKSIIEKLSKKTVIILR
                     SNPNKKENSSNIEQYLASSKLFNTLQPYIKSNEKEKEQAATEVFDLLQGQVTVSATKV
                     AKKTNEAPNIVSVISDKQIRDYGRVSLNDILYQLPGFSASQINDRRTVSSRGLYEGWN
                     NNHLLMLVDGVQHNDLFYGSALTWEITPLNMIKSLEVIRGPGSALYGSNATNGVISLN
                     TYSGGDLNGALRVRARIGDYGTKIYDLITGNKGKLFSAVVSYNSYETDGNNYTNYDGS
                     GRKDAFGFLQKFPQRDERNSYYLFTKLEGEGALKGLSFQYHRQKWDYQTFNGWLQNVP
                     DIKDRQSEYRDVFIAKYSKNITDKLSHEYVMRYNNGYWNYNYRAYPNNTTLGETSYPA
                     GVTELLHTSIYNLFLRAQITYLFTGGGTFVAGVEGNRLSYTGDKSHYSNINMNLLGDQ
                     SPNPGGAMLPLGPAMDWIKDKPIYKIAPFAQLTSGKLFNNKIEFTLGVRYDETSIRYR
                     GIDEPYKNWIGTPSYTGLNPFTQTETTYPIPSEYLGPPFVTNEKKTYRRTSPRGGIVY
                     FYSDRLTFKTMIGRAFREPAPGELYGVNTYVGGSNNPRKISPEVIKTAEIAMDWIINS
                     NFNLRINGFNTRFENAIDYSGTSNSVINAYTIGTRGIESELLMSFKYFSAFINYSRFY
                     RFLDNNLDGTIAKSPNEVTIAPASNANAGITSTFANWIGAITVQRQGAVQRRRSDLGT
                     IDPVTGILLENTYSNPYSYPQYRSKTVPAWVSVNLRLSYKFTEDIQLGLYITNAFNTH
                     QTLVQRANYPFDYIREGRRVMFDFQATF"
     gene            complement(6648..6720)
                     /locus_tag="PLF66_16725"
     tRNA            complement(6648..6720)
                     /locus_tag="PLF66_16725"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6685..6687),aa:Ala,seq:tgc)
     gene            complement(6749..6822)
                     /locus_tag="PLF66_16730"
     tRNA            complement(6749..6822)
                     /locus_tag="PLF66_16730"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6786..6788),aa:Ile,seq:gat)
     gene            complement(6883..8082)
                     /locus_tag="PLF66_16735"
     CDS             complement(6883..8082)
                     /locus_tag="PLF66_16735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003006165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HMZ65738.1"
                     /translation="MDNALFKIVNFTPGSYITLEGERKPSPNFFIIKEGKVNLKRSFP
                     VANEKSSEVIGPGDFFGVISAMSQYPQIESAIAMSNVTLISVGQHRFGDLIQKNSPLA
                     MKIIRFFSKKLRDFDQGQSNKTIGSKVSGEEDLSLLYEMGENYFTQGNIDSAVYLFQS
                     YLKYVPKGEFTEKAKAQLKKLGRPLEMQAVNGANRSYMQNQMIFSENEPGQDLFILQK
                     GKVKITKIIKGTDVLLNIMKPGDIFGEMALLDNKPRSASAIAMEDADLLAINKANFES
                     MVQTQPQLMSKIITVLSERVWNAYKKIANSMIEDMNGRIADMLLTIVEKNRVKIAPKA
                     GYDFQLSIFDLLKMLGLTDRDTNLIMRFMTTNKFIYTEGDNLLCSDLALLDRLVHYHK
                     EGKQRRT"
     gene            complement(8172..9722)
                     /locus_tag="PLF66_16740"
     CDS             complement(8172..9722)
                     /locus_tag="PLF66_16740"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HMZ65739.1"
                     /translation="MKAYIRTRLLLGIILSALLVSGLLGIFIYKQSADIFIEINQRVQ
                     GEVATNTDLPLEIKNRIMNSAMIASILILFFILVFSFFQVNHILKSLQILSEEIDKLQ
                     ASDYNYTIQFRKKDELGMIAEVLNQMSIRMKQLKEDTNQVSKVKIITQNLKDEKKNFS
                     QYLSNKIKSELNAILINQTPIDSSQENITTSEYMNFYLRNLLLNVNDILEFYQLESPK
                     IQFDKKEFNLTQLIEIIRRVIFPMALEKKLEFIIDIDKRIPKQVIGDPSRTFLILMNL
                     LNNSIKFTKEGSIHLFLKLNHIENNYVAIDFSIVDTGIGISSDKLNQIFNSNPYLSNG
                     FGLRLSIRILEALGSKIFVESQIGIGSNFSFTLQFEFKKDEINQTESEKKFSPPPLSN
                     IKLLLVEDYKLNQIVVEEFLAIWNVEVDIADNGMRAVEMTKNKDYSIILMDLQMPILD
                     GYEAAKMIRSLPDEKYKSIPIIALTASAINDTMIKVKEAGMNGYITKPFRPEELYQVI
                     VENTSTKL"
     gene            complement(9719..10234)
                     /gene="msrA"
                     /locus_tag="PLF66_16745"
     CDS             complement(9719..10234)
                     /gene="msrA"
                     /locus_tag="PLF66_16745"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002747466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HMZ65740.1"
                     /translation="MKNKIVLGGGCFWCLEAVYQLVEGVTSVVSGYAGGHKINPTYKE
                     VCDETTGHAEVVQLEFDSEKTSLSKLLEIFWKIHDPTTLNRQGNDVGTQYRSIILYED
                     ANQKEIAEASKKDAQSNWNKPIVTEIVPLTTFYPAEDYHQNYFNNNPYNSYCAYVVKP
                     KVDKFKKSLSQ"
     gene            complement(10309..11502)
                     /locus_tag="PLF66_16750"
     CDS             complement(10309..11502)
                     /locus_tag="PLF66_16750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65741.1"
                     /translation="MLFLFLLLLSFPVFGQNQNEDSIDSILSKIYAERDSFYADSFYE
                     ETLEKAYHAYLEKDFKRAIQKLDLIYSKYKDRDWRYYSIRGKVHEAKEEEDEAIYHFT
                     FSIALNPAQPEIFKKLYELNFKIRRPVKSFDYIRLYLKENENDIQMRYRALILAKRLG
                     NDEYVRYSLKKIQNLTPKELDINQINKEISDLISKNKIKEAKIEVNQYLPHFPINEEL
                     HSNLYIILRKLKVKDREIESALMDSAVLLSENTKYPLRLAMFYKERKRYLQALDLFRR
                     IFYTTLSKQGFEMDTEIILFLKECYSELNQSNDVRATLSLMEIFSKKEKPEFGEWNSL
                     RINFQNNREFLVSLLYYTKSNQMNVQYDSIKSILVDRDNKKADSERMNIYSVFTYDDF
                     RNQIP"
     gene            11874..12323
                     /locus_tag="PLF66_16755"
     CDS             11874..12323
                     /locus_tag="PLF66_16755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65742.1"
                     /translation="MSKTNLKVKKNKKKLKSTWFELISNKEEFKNIVNILNRYYNLNP
                     TKNHPSPAHLFREEVVQTDEGNLRIFLKKFGEFEFLIVVEISSASGKKMDSWIHIDGI
                     AQERLALSSQGISNHPVFQIISMDDLFQKNCQEVERGFRPKAEEAIA"
     gene            complement(12374..13585)
                     /locus_tag="PLF66_16760"
     CDS             complement(12374..13585)
                     /locus_tag="PLF66_16760"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008593161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HMZ65743.1"
                     /translation="MQNLVVQKYGGTSVGTTDKIKNVANRIKSYHDKGSQVVVVVSAM
                     GHTTDELVDLAEKINPNASKREMDMLLSTGEQVSIALLAMALEAIQVPAQSFTGAQVK
                     IITDGNFSNAKIEMIDRTRIDEALSQGKVVIVAGFQGIDRNENITTLGRGGSDTSAVA
                     LAATLGAKECEIYTDVDGVYSADPNKVQGAKKHSMITYDEMLELASLGAGVLHSRSVE
                     FAKNYDVVIHVRSSFNQNPGTLVVSEEKLVEKVRVSGVTTKSEEARVTLPDVPDKPGI
                     AAELFGSLADADIIVDVIVQSSPYNGKNTISFTISRKSIKDAVPILEKFAKDHGTGKV
                     DIDDKIAIVSAVGVGMKSHVGVAATMFKALAANNINIEMISTSEIKISCVIKETDAKK
                     ALQEIHKAFDI"
     gene            complement(13634..14035)
                     /locus_tag="PLF66_16765"
     CDS             complement(13634..14035)
                     /locus_tag="PLF66_16765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65744.1"
                     /translation="MTDLNKGDNIRYPLSFHWFSKLNLTRFIPWHFDSELNLDPNRSI
                     NQRFRKECKNREVITFGHRQDQDTFAGFEIINQTIQEKVLVFHPSFGENVDGWNIVEA
                     EYSNFFEFLKKEVLPTMEEWIEEDDVEDYLD"
     gene            complement(14028..15224)
                     /locus_tag="PLF66_16770"
     CDS             complement(14028..15224)
                     /locus_tag="PLF66_16770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65745.1"
                     /translation="MTKKTESNNEHQFSTNLNRVLAKIQKGNRAFTDPEVKEFCAKRW
                     RNIFINEAVQDPIAMKAATEKPLFGKDNRPPKQKIYERFLRNDGPAEWNIDYPEARNP
                     VLKNTTLLFCPGLLTGLLPVMAFQEAFPAVEKKYKIKVLQSDSHPMRGCDANVNDIRN
                     AVDKGIGLDSHAKPITKGKPPKDVFVICYSKGMADLLTALIAMPELKDRIRCIFNWAG
                     APGGSPLADNIYSSIKDLDMSALKNLTDVLKVVNPFVNLGDKVRRMDEYHIKDAVESI
                     TTTYRQAFLQKHLKEIDKMNIPIFNITGSTTVTEVPYFQMQGVMELNKYDANNDMQVT
                     QNGAKIASPMATDLAMLHGHHWDLSYSPFPKNMRFGSAHLDHPFPKEAALTAMIKFAF
                     ELGLID"
     gene            complement(15307..16767)
                     /locus_tag="PLF66_16775"
     CDS             complement(15307..16767)
                     /locus_tag="PLF66_16775"
                     /inference="COORDINATES: protein motif:HMM:NF003715.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HMZ65746.1"
                     /translation="MDLLNQFLLGTSILLLLSIVASKATGKFGIPTLLIFLFIGMLAG
                     SDGIGGIYFDNSQITQNVGIVALIYILFSGGLDTHWSKVKPVFWQGFSLATLGVLITA
                     IAVGLFVYQFLNFSLFEGLLIGSIVASTDAAAVFSIMRSQKVNLQGQIRETIELESGS
                     NDPMSVFLTIALLRLVKNPDSSLVDLFLLFFQQMLIGGISGYILGKFSIFIVNKIKLQ
                     YDGLYPVLTISLALFSYSITSYIGGNGFLAVYLTGLFLSDKDFIHKKSLIRLHDGLSW
                     LMQIIMFLLLGLLVFPKQLVSVIGMGLILSIVLIFLARPLSVYISLIFSKLNYKQKGI
                     ISWVGLRGSVPIILATFPKEAGIEKADLIFNLVFFIVITSILFQGTLLPKVANLLNVL
                     DKEDTKRIQIPSIHLDDIGKTDNDLVTITLGKDSTLIGKQIVEAGLPEGALIVLLTRG
                     EEYLVPRGSTQLLENDTILILASRRLLPTISQIFKT"
     gene            complement(16818..17585)
                     /locus_tag="PLF66_16780"
     CDS             complement(16818..17585)
                     /locus_tag="PLF66_16780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65747.1"
                     /translation="MNLKNFLLILISILFTILGFIIFFNARTDEERNLGIGSIGLFGL
                     TAVTFVYFTWRKKVFQKQINEDLKEVSIEGGRKFHVNKVNFYLISVLIFFLGGFLAYF
                     VRLNLVFMIASALMSVLGFIVFFGVLFGFFAKEYLAFEEDGIRMGFRNYSFIIRWDNV
                     MKISSDEWHSHMATFIYLINPEDTTRYLYVTRGRRDKTIKKIYNKIGWNLTMTGNHIL
                     ILPERYGLDAGYFFKMLEGYLKFPETRKDLKIKKKIE"
     gene            complement(17582..19003)
                     /locus_tag="PLF66_16785"
     CDS             complement(17582..19003)
                     /locus_tag="PLF66_16785"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65748.1"
                     /translation="MELTIFIITLFAFIFIGIKESRKVKDDSSYLLANRKTGLFALIA
                     TLVMTEFNTSTLLGFSSAGYTTGLWGLTLPFVFLIGLFFYTFTVAKKWKKLNGMSVAE
                     LFTLRYGKTIGSIASMFLILAMIGFSATYVKSMTTIFQPFVPNWNPYFISALLVIVVL
                     FMTLRGGLVSVISTDILGFLGTILVIPLLLYFSYKQKSAGIEEIIQVFPPESSNALPI
                     RYIFSLILLTMFTYIAAPWYGQKIFAAASEKTAFIAVGISSVIIFLLYAFPILALTYL
                     RVNGVVGINPEEGIPYIISNYFPSGLKAFAFAILFAAAATTLSGIWSALTTMLVGDYL
                     LSPKNPKLGKDYVSSMKIYLTFAVLSWILGNIFVDKILNKLILANIPIAALSFSLLAG
                     FYWEKASRRGAMISITLGLLWGSGCYLILGDEGGYTWYWAIYGIPIIFLSGILASLIL
                     PQTEEEKKDLEKFKIRMDAKENQ"
     gene            complement(19013..19615)
                     /locus_tag="PLF66_16790"
     CDS             complement(19013..19615)
                     /locus_tag="PLF66_16790"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HMZ65749.1"
                     /translation="MKEIFLLPNLLTLSRILLILPSIYFIEKVDLILSVVFIGFLLIT
                     DFLDGFLARKLNQTSSLGAVLDPIADKLVVIAFFLHLLFRNSVLEFYVYLVLTRDLLQ
                     LSVIPVLSWWKKIPFKVKPKLIPKIGTALNFILIGLYEFQFIFSKLPELLQVYIDKIE
                     MINYVILGLLLISIPIEVYILITFIPRFIQIYKGTHDTFE"
     gene            19990..20457
                     /locus_tag="PLF66_16795"
     CDS             19990..20457
                     /locus_tag="PLF66_16795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65750.1"
                     /translation="MSLIRISYTSKKQIELDERYTHHNTEIVVHAVLKLILGLMVSVS
                     VLAQSLIYDSGLCYMRTYRQICTCNHNSKKEIHRQTNKSDCHDKSKSVHICSCKKSKN
                     PNELSNLLKQIFFLWQGNQDLALEFIKFNYSTLLKDVILNGYGLTLIKPPRLS"
     gene            20563..21492
                     /locus_tag="PLF66_16800"
     CDS             20563..21492
                     /locus_tag="PLF66_16800"
                     /inference="COORDINATES: protein motif:HMM:TIGR04052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallo-mystery pair system four-Cys motif
                     protein"
                     /protein_id="HMZ65751.1"
                     /translation="MKYVITISIILISLSCNKFAANNNEDKNKNIAAATILLNQRKST
                     AASVSSINFNVVSGTTNVSCSGSIAGTIAGATNAKLVDLKFYVHDIKLISSDGSKVDF
                     SITTDNKWQMAAGSNAFGSYPGVALLDFEDATGNCSSGTTETNKTIYGTSAVGNYVGV
                     EFKVGVPFYLNHMNVSTAASPLNISAMYWAWASGYKFAKIEFLANSNTNMFHLGSGSC
                     SGNSTGPVNPCGLPNRPTISLTKTSGGFDATRDTITLDLNTLYNGADASGTGITNCMA
                     GNGIAACQPIITNIGITVSTGASSGLQTAFSLK"
     gene            21548..22777
                     /locus_tag="PLF66_16805"
     CDS             21548..22777
                     /locus_tag="PLF66_16805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016549304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme enzyme"
                     /protein_id="HMZ65752.1"
                     /translation="MRTSKKYILNIIKIYVMSLSIVSISFCQNLTHSDSNKVNDQNTK
                     LTLSYLAISRSSASYTWDLPATFPTPRVPSENPMSNAKVELGRFLFYEKKLSQNQTQS
                     CGSCHLQEFAFSDRKPFGVGSTGEFHPRNAQHLGNVAYNTKVTWANTNMGSLEVQARA
                     PMFGISPIELGLTNDTYLDRLKADTNYRNLFSKAFGGGTENITEQNVRFAISSFQRSL
                     ITGRSPVDKYLNYGDKTALSASAIRGMSIFNGETAECFHCHGGFNYTNTVNHTGTVFE
                     EVSYMSNGIISDATYATLPSNKKGLYDLTGLATDIGRFKVPSLRNVALTYPYMHDGSI
                     TCTVASASNVDACSTEALGKVIDHYMSGGQSHQNKDPQIRAFSLTPQEKTDLISFLKA
                     LTDDEFINNSKFSDPFK"
     gene            22774..23649
                     /locus_tag="PLF66_16810"
     CDS             22774..23649
                     /locus_tag="PLF66_16810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65753.1"
                     /translation="MKIIQTILIILCLESCTFGSEGNSLTDKKIIITRFALLRDSIKT
                     NASLQLLFYDDQGDENLNTFQLNSANTSDIYTVTLVDTSKISFDKIELYVNTSSATVL
                     ESNTSPPEVFFHEADSGPTGSASATTKVYVTANGYATSTFLNANGSDELGSYSATDNF
                     VLTNLPQGILQKVTFHFGSIVLVGSLKSTTKTKSFTIQFPSRDLSFSRLCSNLLGLNQ
                     NLNLKLDIRYSDLFKDTTTNTYLTIDNTKILRAIHDLSDPNPTISITQNSGIFNEINK
                     NWNLTTIIKEHRCSK"
     gene            23646..24578
                     /locus_tag="PLF66_16815"
     CDS             23646..24578
                     /locus_tag="PLF66_16815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65754.1"
                     /translation="MIYSRKYFLLAATIYYLLVTLSLYSHHTGSGLDTTQFNVRFLGK
                     EEDKGGFPPTYFIVSNEYSRGAQENRNIDTTNMYGESIFMRGLFTVNANFSYIYYDQK
                     DRSDFARYGKPYIGSKIFPFYKTQAENSFFFFLEGRIGFPTSAVNQKFIDSNYYTAQG
                     NTAFGYLWKSISIVGKLGGEMPISRYTGDRSAEYDEVPYILRPPQTFTWNQIDNIQLK
                     KTTNASGFITYIYNNHLSLFGGYLYRTPFNGIQKDRTTGDKIPLIFREVSFGLNYKFS
                     EKYMLTVTYRNPLYRGRDFRPYESSINIAFTFNL"
BASE COUNT         8160 a         4034 c         4221 g         8206 t
ORIGIN      
        1 gatagcgaat ttcatttctt caatgtccgg agagattcaa agtgtatttg tagatatata
       61 aatggtatgc aaccaattac agttgggcgg tgctcctccg ttcgaatatt ggaaaagggt
      121 tgcttaattg agattttttc aactttcact tggtagaata tgggggcatg ttagctcgat
      181 tgtaaaagtt ccattcaagg gaggttgtca cacctataca agaattttga aaacaaaatt
      241 tactttttat gatgaaaaga acaaaaaagg aaatcgataa cattctagaa attcttgcaa
      301 aacccattct ttttatcaca ggtgtgaatg attatgcagt tacaaaaggc ttttggatta
      361 aggttggtaa attaccgtta gatgaaagat aaatcaatct gcctcccaaa tttatacaag
      421 atgctttaaa ctccgaaaga ctttttattg ttgaaaatgg agttcgctgt cccgcatcaa
      481 aagaagatgt gcaggattcg aaagagttgc cgtatggaat ctggaaggaa ttcaaaacag
      541 attaaatgat tattatccaa gacgaaaaag aatcagtaga agcagagaaa aatccagaaa
      601 tcctttttaa gaaaaaagta aaagtggtca aagaagtaat gattaaacgt ttttaaaaaa
      661 gaataaagga atgcgttttg aatgccatag attcaagttg gctaaaaaaa caataaacga
      721 agcatcacac ccaaaacctt aacaaactct ccaaattttt atccataacc atccgtggtt
      781 atctctcaac atcctcctaa aagaaacaat attttgcaac ttacaaatag accctacttt
      841 cccttaaata atgtttttca aaattatgac cgatggaaag attagagtat gaaagtaaaa
      901 atgttttatt ttatcctaat tcttggactt gttttagaaa ccagtttatt cgcagaagta
      961 gatttgaatt acaactatga ttatgagatg ggtggagtca aaaccaagtt ctcagaatgg
     1021 gttccttata aattacacaa atggaatcca aaatttttag aagattatta tgagttgtat
     1081 ggattgaagc ttcattatgg agaagaagaa cttagaaagg atatttattt tttaaaaata
     1141 ggattagaaa gtcggtttcg tcatccaaat aattctttgt gtagagtaaa aaatgagaga
     1201 gagtatcata agtatagact tttagtttct atgcacatga atttacaaat catgagagct
     1261 tatatgcgaa tcgcttctca atacgataag cgacatgttt actttcaaaa tttagattat
     1321 gctcatgatt taaaaaaatc ttttaaaata gcagagggct tttataaaga agcaattcct
     1381 tattggaaaa aagctgagga acttgccaaa aaagccaatg agataaaaga tgaaattgat
     1441 ttaggaacta tggagtctga gagatttgaa atcagcaatg ggaaattaaa ttttggaaat
     1501 ataatcaacg atcatctttc tagattagct aagaaacaag aaactgtaga agaatattta
     1561 aaacaaaatc cggagcagga taaacctcct ctttaatata aaccacttac attatggaaa
     1621 atggatattc tctcgaatag aagaaggttt ttagaatatt agagaatgct aataaattaa
     1681 tatattatta gcctgcggct tgcaagccct ccgttctatc tctttcgctc tcctcaagca
     1741 attgtccttt ttcaatgaca atcagagcat catctgaacg cttggctcac cttgtataag
     1801 gtgagttatt catgatttaa aaactgaatc gagctgatta tatttgtaat gataaccgat
     1861 agttctctat caggagtctc gtcgctgtta taagtgcaaa caatcagttt tgagccagaa
     1921 gaaatcatat aaacaagcca aaatctttcg tctgcaataa actcgcaggc tttgatttga
     1981 ctgcctagat tatttgtaaa tgttgcgact ctttcttcgt cgtaagaaat tttatggacg
     2041 gataaaaatc tttccaattc ttcagataaa tcataggaac ctagtttgtt ttcaaacgcg
     2101 gagacttgga gtgcaccggc tccatctgga tcaaaaaaag ctggaatttc ttcaatgatt
     2161 tccacttccc aatgctccgg ataagcaagt atataccatc catttggaga tctataaggt
     2221 tgatagtgta tttcgctcat ttctaaattc ctttacttga caatgcaagg aaacaagaat
     2281 ctttaaatta tgtgaatcaa aaaaaatcga attccaaaaa attttctttt ccaacgatga
     2341 tggttttatt aattttattg agcattgttg gtggatttgt tttattttta ttatcagtca
     2401 tcctactgat cgcatttctt ttttatccta tttataaaag atatttttta cgtaagtatc
     2461 ctgttcaaga tattgcggat gaagtctctt atgcagtcac tgaggatgga tggaatcttg
     2521 ctattcatag acataaacct atgaagccac tccccaatgc ttatccagtg attgtagtcc
     2581 atggaattat caccaacaaa tactttatgg atttagatgt ggatcattct cttgcctatc
     2641 atttaaagct ttgcggctac gatgtgtttg ccgtatctct cagaggatgc ggagaatctc
     2701 ataaaaaatc ggaagatcaa aattttaatt ttgatgatat ggttatgaaa gatgttcccg
     2761 ctattatcaa agaagtaagt aatatcactg gtaaaaacaa aatcaattgg attgctcatt
     2821 caatgggtgc tatgattatg tattcctatc ttggtgttgc acccaaaaaa gaaaaagata
     2881 aagttaaaag ttttatttcc cttggaggac ccggcaattt aagtcattct agtcctgtgc
     2941 ttggaaaagc tggtgtgaag tatatctctc ttgcgaagaa gattgattta aagctcatgg
     3001 ctaaatttat tttacccttt gtaagtattt ttaattctcc cctaaaagaa attttttata
     3061 atccccaagt aactactaaa aacactgtga agaaaatgct ttttggaatt gaaaatatcg
     3121 cggacggtct catgcttcaa actctagaat ggatggcaaa tgggggagat ataatttcca
     3181 ttgataaaaa attcaattat acagaactac aaactaagat tacttgtccg attcttttta
     3241 ttgctggtgg atacgacaat gtagccactc ccaaaagttt aaaatctgtt tataataaag
     3301 tgagttctaa gtataaggaa ttccatgttg tctctgaaag tgctggattc agcggagact
     3361 atgggcatgc ctgtcttgta atgggacaaa atgctaaaaa agaaatcttt cctcttttgg
     3421 aagcatttct tctgaaacat ggaaaatcaa aatagaattc tttttccaat cattcactca
     3481 acaaacaaat agaatggttt gcttggattg agtgaatgtt tatttttcta gtaaattctt
     3541 tgtttaaaaa taataagatt cctttggtct atccaatacc agccaagtaa aatccttttg
     3601 ggaaaatact ttcgtttctc agttatttgg tatctatcaa ataatcactc tttgcccctc
     3661 tcgtattaaa aaaagcttta ccttttttaa aaacttgtat cctattacat cgactaaacg
     3721 ttatcaaaag aaatcagaac cattccttga tattagatta gttccttaga aaaactatga
     3781 atcaaatcaa attaaaaatt tacattttta ttcagatctt atttcttctt cctgtttcag
     3841 cacaagaaac aactcaaaca aataaggtca tcattggaaa gattcagcct tacgaatctg
     3901 aaacaaacca gaatttagaa aaagagattc aatctactct agcatcaaaa ttacaagagt
     3961 ctaaattttt aatttctaat tcgactgaat caactctacc ttccagatta aaagaagcca
     4021 agatggctaa tgctcgttat ctcctagaag gcttttacaa taaaaaatcg aatgattcta
     4081 atttaaattt atacattcaa atttatgatc cacagacaga gagaatgatt gatgcttata
     4141 gtattacaga cgaagtattt caatcagaag ggttgcaatt ggataaagaa gaactcaaag
     4201 agaatgacaa aagtatcatt gagaaattgt cgaagaaaac agtgattatc ctcagatcga
     4261 atccaaataa aaaagagaat agttcaaata tagaacaata cttagcttcc tctaaattat
     4321 ttaatacttt acaaccatat attaaatcaa atgagaaaga aaaagagcag gcggctacag
     4381 aagtttttga tttactccaa ggtcaggtga cggtttccgc tactaaagtt gccaaaaaaa
     4441 cgaatgaggc tcctaatatc gtatccgtaa tttctgataa acaaattcga gattatggta
     4501 gagtttcctt gaatgatata ctctaccaac tcccaggttt ttctgcatca caaatcaatg
     4561 atcgtagaac tgtttcctct agaggtcttt atgaaggctg gaataacaat catttactta
     4621 tgcttgtaga tggagtgcaa cacaatgatt tattttatgg ttctgctctc acttgggaaa
     4681 ttactccact caatatgatt aaatccctcg aagtgattcg aggtcccggt tcagccctat
     4741 atggatctaa tgcgactaac ggtgttatct ccttaaatac ctattctgga ggtgacttaa
     4801 atggagcgtt aagggttcga gctagaattg gagactatgg gacaaaaata tatgatttga
     4861 taacaggcaa caagggaaaa ttattttctg ctgtggtgag ttataattct tatgaaacag
     4921 atggaaataa ttatacaaac tatgatggat caggaagaaa agatgctttt ggctttttac
     4981 aaaaatttcc gcaacgagat gaaagaaata gttattactt atttacaaag ttagaagggg
     5041 agggcgcatt aaaaggactc tcctttcaat accatcgaca aaaatgggat taccaaactt
     5101 ttaatggttg gcttcaaaat gttccagata taaaagatag acaatctgaa tatagagatg
     5161 tatttattgc taaatattcg aaaaatatta cagacaaact gagtcatgaa tacgtgatgc
     5221 gatataacaa tggatattgg aattataatt acagagcata tccaaacaat accacccttg
     5281 gtgaaacttc ttatccggca ggtgtaacag aacttttaca tacaagtatt tataatttat
     5341 ttttgcgtgc acagatcaca tatctattta caggaggcgg aacatttgtt gcgggcgttg
     5401 aaggaaatag actttcgtat acaggagata aaagtcatta tagtaatatt aatatgaatt
     5461 tgcttgggga tcaaagtcct aatccaggtg gtgctatgtt gccattgggt cctgctatgg
     5521 attggataaa agataaaccg atttataaaa ttgctccttt cgctcagcta acaagtggaa
     5581 aactctttaa taataaaatt gaatttactc taggtgttcg ttatgatgag acatctattc
     5641 gatatagagg aattgatgaa ccctataaaa attggatagg cactccttct tatacaggtt
     5701 taaatccatt cactcagact gaaactactt atccaattcc tagcgaatat ctaggtccgc
     5761 ccttcgttac aaatgagaaa aagacataca gaagaacaag tcctagaggt ggtatcgttt
     5821 atttctattc agaccgacta acgtttaaaa caatgatagg acgtgctttt agagagcctg
     5881 cacccggtga gttgtatggt gtaaatactt atgtgggtgg atccaataat ccaagaaaaa
     5941 ttagtcccga ggtgattaaa actgctgaga ttgcgatgga ttggattatt aattctaatt
     6001 ttaatttaag aatcaatgga tttaatacac gttttgaaaa tgcaattgat tatagtggaa
     6061 cttcaaactc agttattaat gcttatacaa taggcaccag agggattgaa tcagaattgc
     6121 taatgtcttt taaatatttt tctgctttta tcaattactc tcgcttctat cggtttttgg
     6181 ataataattt agatggaacc attgccaaaa gtccaaatga agtaacgatt gcgccagctt
     6241 ccaatgccaa tgccggtatt acttcaacct ttgcaaattg gattggtgcg attaccgttc
     6301 aaagacaagg ggctgttcaa agaagacgca gtgatttggg aactatcgat cctgtaacag
     6361 gaatattatt ggaaaatact tacagcaatc cttattctta tccacaatat agatcaaaga
     6421 cagtccctgc ctgggtgagt gtaaatctga gattatctta taaatttaca gaagatattc
     6481 aattgggttt atatataact aacgcattta atacgcatca aacattggtt caaagggcaa
     6541 attatccttt tgattacatt cgagagggta gaagagtaat gtttgatttt caagctacat
     6601 tctaaatgaa agatttaaag tattagggga ggaatggaaa ttgaaattgg agcttatcgg
     6661 gatcgaaccg atgaccctct gcttgcaaag cagatgctct cccagctgag ctaaagcccc
     6721 ctataaaaat ctattttcaa tttacttctg ggcctgacaa gatttgaact tgtgacccct
     6781 cgcttatcaa gcgagtgctc taaccagctg agctacaggc cctaaagata tatgtcagtt
     6841 ttttacagaa ctgttttttt tcaaattctt ttataaaaaa aattaagttc ttctctgttt
     6901 tccttcttta tgatagtgaa ctaggcgatc taggagtgct agatcagaac aaagcaggtt
     6961 gtcgccttct gtgtagataa atttgtttgt ggtcataaat ctcatgatta aattcgtatc
     7021 tcggtctgtt aatccaagca ttttcagaag atcaaaaata ctcaattgga agtcatagcc
     7081 tgcttttggt gctatcttca ctcgattttt ctctacaatt gtgagaagca tatcggcgat
     7141 tcttccattc atatcttcta tcattgaatt tgctattttt ttataggcat tccagactcg
     7201 ttcggataat acagtaatga tcttactcat aagctgaggt tgggtttgca ccatagattc
     7261 aaaattggct ttgttgatcg caagtaaatc ggcatcttcc atagcaatcg cagaagcaga
     7321 ccgaggttta ttatctagaa gtgccatttc accaaaaata tcacccggct tcatgatatt
     7381 cagtaataca tcagttccct tgataatttt tgttatcttt acttttcctt tctgtaagat
     7441 aaatagatct tgtcctggct cattctcact aaaaatcatt tgattttgca tatagcttct
     7501 attggcacca tttacagctt gcatttctag gggtctacct agctttttta actgagcctt
     7561 tgctttctct gtaaactcac cttttggaac atactttaag tagctttgaa ataaatagac
     7621 tgcactatca atatttccct gagtaaaata gttttctccc atttcataca atagacttaa
     7681 gtcttcctca ccggatacct tagagcctat cgtcttattg gattgccctt ggtcaaagtc
     7741 tcttagtttt ttagagaaga agcgaatgat tttcattgct aaaggagaat tcttttggat
     7801 tagatcaccg aagcgatgtt ggccaacaga aatcagcgtt acgttactca ttgcgatagc
     7861 agattcaatt tgtggatatt ggctcattgc ggagatgaca ccaaaaaaat caccaggtcc
     7921 tattacctcg ctcgactttt catttgcaac aggaaagctt ctttttaaat ttacttttcc
     7981 ttctttgatg atgaaaaaat tgggagaagg ttttctttcc ccttctagag tgatataaga
     8041 tccgggagtg aaatttacta ttttaaaaag ggcattatcc ataggtatac tttaaactat
     8101 tcagtcataa attttacaat caaaactttt ctttttattt ctagaatctt tctaagaccc
     8161 taagaccagt tttaaagttt agtcgaggta ttttcgacta tcacttgata cagctcttct
     8221 ggtctaaatg gttttgttat gtaaccattc attcctgctt ccttgacctt tatcattgta
     8281 tcatttattg ctgatgcggt taaagctata attggaattg atttatattt ttcatccggt
     8341 aggcttctga tcattttggc tgcttcatat ccatctagaa ttggcatttg caaatccatt
     8401 agaataattg aataatcttt gttttttgtc atttcaactg ctctcatgcc gttatccgct
     8461 atatcaactt ctacattcca aatggctaaa aattcttcta ctacgatctg attcagtttg
     8521 taatcttcta ctaataaaag ctttatattt gatagagggg gtggagaaaa ttttttctct
     8581 gattcagttt gattgatttc atctttcttg aactcaaatt gaagtgtaaa agaaaagtta
     8641 gatccaatac caatttgact ctcaacaaaa atcttacttc caagtgcttc tagaattcta
     8701 atcgatagac gtagaccaaa accattcgaa agatagggat tactattgaa gatttgattt
     8761 agtttatcac tgctaatccc aatacctgta tccacaatag aaaaatcgat tgcaacataa
     8821 ttgttctcta tgtgatttag ttttaaaaat aaatgaatcg atccctcttt tgtgaattta
     8881 attgagttat tcagcaaatt cattaaaatt aaaaacgttc tagatggatc tccaatgact
     8941 tgttttggga ttcttttatc tatatctatt ataaattcta attttttttc aagagccatt
     9001 ggaaaaataa ctctgcgaat gatttcaatt aattgtgtta aattaaattc ttttttatca
     9061 aattgaattt taggcgattc taattggtaa aactctaaaa tatcatttac attgagtaaa
     9121 agatttctta ggtaaaaatt catgtattca cttgttgtaa tattttcttg tgaagaatca
     9181 atcggagttt gatttattag tattgcattg agttctgatt tgattttatt acttaagtat
     9241 tgagaaaagt tctttttttc atcttttaag ttctgagtga tgatcttgac tttcgatact
     9301 tggttcgtat cctcttttag ttgtttcatt cgaatcgaca tttgattcaa aacttcagca
     9361 atcataccaa gttcatcttt ttttcgaaat tgaattgtat aattataatc gcttgcttgt
     9421 agtttatcaa tctcttcaga caaaatttgt agagatttta atatatgatt tacctgaaaa
     9481 aaagaaaaaa ccaaaataaa aaacagaatt aaaatggaag caatcattgc agagttcatg
     9541 attctatttt ttatttctag cggcaaatct gtatttgtcg ctacttcacc ttggactctc
     9601 tgatttattt cgataaaaat atctgcagat tgtttgtata taaatatacc aagtaatcct
     9661 gagactaaaa gtgcagatag gattatacct aaaaggagtc ttgttcgaat gtatgctttc
     9721 attgactcaa agatttttta aatttatcta ccttaggttt cactacatat gcacaatagg
     9781 aattataggg attgttatta aaataatttt gatgataatc ttctgcagga tagaaagttg
     9841 tgagaggcac gatttctgtt acgattggtt tgttccaatt tgactgcgca tcctttttag
     9901 aggcttccgc tatttctttt tgatttgcat cttcatagag aatgatagat ctgtattgtg
     9961 tgcctacatc attgccttga cgatttagag tagtgggatc gtgaattttc caaaagattt
    10021 ctaatagttt agacagggag gttttctctg agtcgaactc aagttgcacg acttctgcat
    10081 gaccagtggt ttcatcacat acctctttat atgtaggatt gattttatgt ccacctgcat
    10141 atccagaaac gacagacgta actccttcaa caagttgata aacagcttcg aggcaccaaa
    10201 aacaaccacc acctaatacg attttgttct tcataaatta ccacttttta ataatttgag
    10261 tttatgaatt agtatatttt tttgaactgg ttaggcaaga atttttattt atgggatttg
    10321 atttctgaag tcgtcatagg taaaaacgga atagatattc attctctccg aatcggcttt
    10381 tttattgtct cgatctacta gtatagattt tattgaatca tattgaacat tcatttgatt
    10441 tgattttgta tagtaaagta aggaaactaa aaactctcga ttgttctgaa aattaattct
    10501 aagagaattc cattctccaa attctggttt ttcctttttg gaaaaaattt ccattagaga
    10561 taaagttgct cttacatcat tggattgatt taattcactg tagcattctt ttagaaatag
    10621 aatgatttca gtatccatct caaatccttg tttggagagt gttgtataaa aaattcttct
    10681 aaacaaatcg agagcttgta aatatctttt tctttctttg taaaacattg cgagccgcaa
    10741 aggatattta gtattttcgg aaagtagaac agcgctatcc ataagtgctg attcgatttc
    10801 cctatctttt acttttagtt ttctaagtat tatgtataaa ttcgagtgta attcttcgtt
    10861 gattggaaag tgaggtaaat attgattaac ctctattttt gcttctttta ttttattctt
    10921 tgaaattaaa tcagatattt ctttattaat ttggtttata tccaattctt ttggggtaag
    10981 attctgaatt ttctttaggg aataacgcac atattcatca ttgccaagtc tttttgctag
    11041 aatcaaggct ctgtatctca tttgtatatc gttttcattc tcttttaaat agagacgaat
    11101 atagtcaaag gattttacag gtcgtcttat tttgaaatta agttcataca gttttttaaa
    11161 aatttctggt tgagcaggat ttaaagcaat gctaaatgta aaatgataga ttgcctcatc
    11221 ctcttcttct tttgcctcgt ggactttacc tcgaatagaa tagtatctcc agtctctatc
    11281 cttgtatttt gaataaatta aatctagttt ttgaatcgct cttttaaaat ccttttctag
    11341 ataggcatga taggcttttt ctaaagtttc ttcgtagaaa gagtccgcat aaaaactatc
    11401 tctctccgca tagattttag aaaggattga atcaatggaa tcttcgtttt ggttttgacc
    11461 gaaaactgga aaagatagaa gtaatagaaa taaaaaaagc atagtaaacc cactgtaggc
    11521 aaaatttata ttttaacgtc agttcgaaaa atttttaaaa agactatcac tgacaggctc
    11581 tgaacctttc gaatgtgcaa acaccaatta catcgattaa aagaaaagat gaggaaaccc
    11641 attcgtctag agtttctcca tcgcatgttc ctctttgatg aagaaatcat ttcttaatgt
    11701 cctaggtgta aatctcattt gaacgtttgg ttcaccttgt gtaagatgag ctattaaaag
    11761 tatcggtttt tatttttttt gcgtaactgc gtagtttctg aaaaaaaaag cgaacggact
    11821 cggagttttt tatccctact ttcatagacc gttacggttg ttaaggagat aaaatgtcta
    11881 aaaccaatct aaaagtaaaa aagaataaaa agaaactcaa atcaacttgg tttgagctca
    11941 taagcaataa agaggaattc aaaaacattg taaacatttt gaatcgttac tataatttaa
    12001 atccaactaa aaatcatcct tctcctgccc atctttttag ggaggaggtt gtgcaaactg
    12061 atgagggcaa tcttagaatc tttcttaaaa aatttgggga atttgagttt ttaatcgtgg
    12121 tggaaattag ttcagcatct ggtaaaaaaa tggattcatg gattcatatc gatgggattg
    12181 cccaagaaag attagcactt agttcgcaag ggatttcgaa tcatcccgta tttcaaatta
    12241 ttagtatgga tgatttattt caaaagaatt gccaagaggt ggaaagagga tttcgcccta
    12301 aagcggagga agctatcgcg tagtagtgga taatccgtat taactgctga aagctaatac
    12361 ggattataga aatttaaata tcgaaagctt tgtgaatttc ttgcagagct tttttagcat
    12421 ctgtttcttt gattacacag gaaattttaa tttcagaggt agaaatcatt tctatgttaa
    12481 tgttatttgc agctaacgct ttaaacatgg ttgcagcaac acccacatgc gacttcattc
    12541 ctactcctac agcagataca atagcaattt tgtcatcgat atctactttt cctgttccat
    12601 ggtcttttgc aaatttttct aatattggaa cagcgtcttt aattgatttg cgagagattg
    12661 taaatgaaat tgtattcttt ccattgtatg gagaggattg cacgattaca tctactatga
    12721 tatctgcatc tgctaggcta ccaaaaagtt ctgcggcaat accaggcttg tccggaacat
    12781 ccggaagggt aactcttgct tcttcgcttt tagttgtaac tccactgact cttacttttt
    12841 ccactaattt ttcctcactt actactaacg ttccgggatt ttgattaaaa ctggatctta
    12901 catgtatcac tacatcgtaa ttctttgcaa attcaacact tcttgaatgt agaaccccag
    12961 ctccaagact tgctagttct aacatttcat cataggtaat cattgaatgt ttttttgcac
    13021 cttgcacttt gttggggtct gcggaataaa caccatctac gtcagtgtag atctcgcatt
    13081 cttttgctcc aagtgttgcg gcaagtgcta cagccgaagt atcgcttccg cctcttccga
    13141 gtgttgtgat attttcgttt ctatcaattc cttgaaatcc ggcaacaatc acaacttttc
    13201 cttgtgagag tgcttcatcg atacgagttc tatctatcat ctcaattttt gcatttgaga
    13261 agtttccatc ggtaatgatc ttcacttgtg cgcctgtaaa agattgtgct ggaacttgaa
    13321 ttgcttcgag agccattgcg agaagtgcaa tggaaacttg ttcccctgta gagaggagca
    13381 tatccatttc tcttttactt gcatttggat tgatcttttc tgcaagatca actaattcat
    13441 cggttgtatg tcccattgca gagacaacaa caactacttg agagccttta tcatgataac
    13501 tcttgatacg gttggctaca ttcttgatct tatctgtagt tcctacagag gttccgccat
    13561 atttttgaac gactagattt tgcataattt tgaaatccat atttttagaa aaatgcttgg
    13621 ggtcaaacat ttatcaatca agataatctt ctacatcatc ctcttcaatc cattcttcca
    13681 ttgtaggaag aacttctttc tttaaaaact cgaaaaaatt tgaatattcc gcttctacaa
    13741 tattccaacc gtctacgttc tcaccaaaag agggatgaaa aacgagaacc ttttcttgga
    13801 tagtttggtt gatgatctca aatccagcga atgtatcttg gtcttgtcta tgaccaaatg
    13861 taatcacttc tctgttttta cactcttttc taaatctttg gttgatgctt cgatttgggt
    13921 ctagatttaa ttcagaatca aaatgccaag ggataaatct tgtaagattt aatttagaaa
    13981 accagtgaaa ggagagggga taacgaatat tatccccttt atttaaatca gtcaatgagt
    14041 cctagttcga aagcgaattt aatcattgct gtaagggcag cttcttttgg aaacgggtgg
    14101 tctaggtggg cagaaccaaa tcgcatattt ttaggaaatg gactataaga taaatcccag
    14161 tggtgaccat gaagcattgc aagatctgtt gccattgggc ttgctatctt agctccattt
    14221 tgcgttactt gcatatcgtt atttgcatcg tatttattta attccataac accttgcatt
    14281 tgaaaataag gaacctctgt aacggtagta gaaccagtta tattaaaaat agggatgttc
    14341 atcttatcaa tttctttcag atgtttttgt aggaaagctt gacgataggt ggtagtgatg
    14401 gattcgactg catctttgat atgatactca tccattcttc tcactttgtc tcctaaattt
    14461 acgaatgggt ttacaacttt taaaacatcg gttaagtttt taagagcact catatctaaa
    14521 tctttgatag aagaatagat attatcagct agaggtgatc caccaggagc accagcccag
    14581 ttgaagatac aacgaattct atcttttagt tctggcattg caatgagtgc agtgagtaaa
    14641 tctgccatgc ctttgctata acaaattaca aaaacatctt ttggaggttt tccttttgtg
    14701 attggttttg catgtgaatc cagaccaata cctttgtcca cagcatttcg tatatcgttt
    14761 acatttgcat cgcatcctct cattggatgt gagtctgatt gtaatacttt gatcttatat
    14821 tttttttcaa cagctggaaa tgcttcctga aaagccataa caggtagtag acctgtgaga
    14881 agcccaggac aaaaaagtaa agttgtgttt tttaaaacag gatttctagc ttcaggataa
    14941 tcaatgttcc attcagccgg tccatcattt cttaagaatc gttcatagat tttttgttta
    15001 ggaggacgat tgtcttttcc aaagagaggt ttttctgttg ctgctttcat tgcaattgga
    15061 tcttggacag cttcgttgat aaaaatattg cgccatcgtt ttgcacaaaa ctcttttact
    15121 tcaggatcgg taaaggctct attgcctttt tgaatttttg caaggactct atttagattt
    15181 gtggagaatt gatgttcatt gttggattcg gttttcttgg tcatagcatc atttggagaa
    15241 atctaatatt tttgtcatgc aagaaattta gaaaggaaga atatttttat atgaaaaaac
    15301 ttttttttaa gttttgaaaa tttgtgaaat agtaggaaga agtcttctac ttgcaagtat
    15361 aagaattgta tcattttcta gtaattgagt tgaacctctt ggcactaaat attcctctcc
    15421 tcttgttagt aatacaatga gtgctccttc aggaagtcct gcttctacta tttgcttgcc
    15481 gattaacgtt gagtctttgc ctaatgtaat tgtgactaag tcgttatctg tttttcctat
    15541 atcatctaaa tgaatacttg gaatttggat tctttttgta tcttctttgt ctaaaacatt
    15601 gagtagattt gcaactttag gcaagagagt tccttggaat aaaatggaag tgattacaat
    15661 gaaaaaaact aaattaaaaa taagatctgc tttttcaatt cctgcttctt tgggaaaggt
    15721 tgctaaaata attggaacag atcctctaag ccctacccat gaaataattc ctttttgttt
    15781 ataatttaat ttggagaaaa ttaaacttat ataaacactc aaaggtcgtg ctaagaatat
    15841 taaaacaatg gataagatta aacccatccc aatcacagaa actaattgtt ttggaaatac
    15901 aagtagtcct agtaataaga acatgatgat ttgcatgagc caactcagac catcatgaag
    15961 ccggatcaaa ctttttttgt gaatgaagtc cttgtcagat agaaagagcc ctgtgaggta
    16021 aacagcgaga aacccatttc ctccaatata agacgttata ctataactaa aaagagctaa
    16081 agagattgtt aagacaggat atagaccatc gtattgtaat ttaattttat tcacaatgaa
    16141 aatagaaaat tttccaagaa tatagccact gattccaccg attaacattt gttgaaaaaa
    16201 taataaaaat aaatcgacta ggcttgagtc aggatttttt accaacctaa gaagtgcgat
    16261 agtgagaaaa acagacattg gatcattgct gcccgattcc agttcaattg tttctctgat
    16321 ctgtccttgt agatttacct tttgtgagcg catgatagaa aagacagctg ctgcatctgt
    16381 ggaggctacg atcgatccaa tcagtagacc ttcaaataaa gaaaaattta gaaattgata
    16441 aacaaataga ccaaccgcaa tcgctgtgat taaaactcca agtgttgcga gagagaagcc
    16501 ttgccagaag actggtttta ctttactcca atgagtatcg agtccgccag aaaataaaat
    16561 ataaatcaag gcaacaatcc cgacattctg ggttatctgc gaattatcaa aatagattcc
    16621 gccaatacca tcagaaccag ccaacattcc gataaaaaga aaaatcaata aagtagggat
    16681 tccaaattta ccagtagctt tgcttgctac aatacttaag aggagtagaa tcgaagtgcc
    16741 taataaaaat tgatttaata gatccataat ttaggaaata gatagtaaac tatccgcctt
    16801 tttgtcttaa ttatagacta ttcaatcttc ttttttattt ttaaatcttt tctagtttct
    16861 ggaaatttta aatatccttc tagcatttta aaaaaatagc ctgcatctaa accataacgt
    16921 tcaggaagaa tcagaatatg attgcctgtc atagtaagat tccatccaat tttgttatag
    16981 atttttttta tagttttatc tcttcttcct cttgtaacat ataaatagcg agttgtatcc
    17041 tctggattga tcaggtagat gaatgttgcc atatgactat gccattcatc agaagaaatc
    17101 ttcatcacat tatcccaacg aatgataaag gaatagtttc taaatcccat tcgaatccca
    17161 tcttcttcaa acgctaaata ttcctttgca aagaagccga ataaaactcc aaagaaaacg
    17221 atgaatccca aaacggacat taaagcagat gcaatcataa aaactaggtt taaacgaacg
    17281 aaataagcaa gaaacccacc tagaaaaaaa ataaggactg atattaagta aaaatttact
    17341 ttatttacat gaaactttct accgccttcg atgctaactt cctttagatc ttcatttatt
    17401 tgtttctgaa aaactttttt tctccatgtg aaataaacaa atgtaactgc ggttaatcca
    17461 aataagccga tagaaccgat tccaagattt ctctcttcgt ctgttcttgc attgaaaaaa
    17521 ataataaaac cgagtatggt aaatagaata gaaattaata taagtaagaa attttttaaa
    17581 ttcattggtt ctcttttgca tccattctaa ttttgaattt ttctagatct tttttttctt
    17641 cctctgtttg tggaagaatt aaagaggcaa gtattccact taaaaaaatt attggtattc
    17701 cgtaaatcgc ccaataccat gtatacccac cctcatcacc taggattaag taacatccac
    17761 ttccccataa aagacctaac gttatgctaa tcattgcacc tcttctagat gctttctccc
    17821 aataaaaacc ggcaagcagt gaaaaagaca aagccgctat aggaatattg gctaggatca
    17881 acttatttaa tattttatct acaaatatat ttcctaaaat ccaagagagg actgcaaacg
    17941 ttaggtagat cttcatactg cttacatagt cctttcctag tttgggattt tttggggaaa
    18001 gtagataatc tcctactagc attgtagtga gagcactcca aattccagaa agagttgtgg
    18061 ctgctgcggc aaaaagaatt gcaaaagcaa atgctttgag tccactcgga aaataattac
    18121 ttataatata aggaattcct tcttctggat ttatcccgac tacaccattt actctcaggt
    18181 aggtaagtgc aagaattgga aaggcataca ataaaaatat gattacactc gaaattccaa
    18241 ctgcaataaa tgccgttttc tcggatgcgg ctgcaaatat tttctgacca taccaaggag
    18301 cggcaatata agtaaacata gtcagtaaaa ttaaagagaa tatgtatcga attgggagag
    18361 cattggaaga ctctggtggg aatacttgaa taatctcttc tatacctgca ctcttttgtt
    18421 tataagaaaa atagagcaat aaggggataa caaggattgt ccccaaaaaa cctagaatgt
    18481 cagttgaaat aacagacacc agtccacctc gaagtgtcat aaataaaact acaatcacaa
    18541 gtagagcact gataaaataa ggattccagt ttggaacaaa aggttgaaag attgtagtca
    18601 ttgatttaac gtatgttgca ctaaaaccga tcatggctaa aatcagaaac atactagcaa
    18661 tagacccaat cgtttttcca taacgcaagg taaataattc ggcgacactc atgccattta
    18721 attttttcca tttctttgca actgtgaatg tgtaaaaaaa gagtccaatt aaaaatacaa
    18781 atggaagcgt taatccccat agaccggtag tataaccagc agatgaaaaa ccaagaagag
    18841 ttgaagtatt gaactctgtc attacaaggg tagcaatgag ggcaaataag cctgtttttc
    18901 tatttgcgag tagataagaa gaatcgtctt taacttttct agattctttt atcccaataa
    18961 agataaatgc gaatagtgta attataaata tcgttagttc catagtttgt tgctactcga
    19021 aggtatcatg agttccttta tagatttgga tgaagcgagg gataaaagtg attagaatat
    19081 aaacttcaat tgggattgaa atcagaagca aacctaggat cacatagttt atcatctcaa
    19141 ttttatctat gtatacttgt aaaagttctg gtagtttaga gaaaataaac tgaaactcat
    19201 aaagcccgat taaaataaaa ttgagagcag ttccgatttt gggaataagt ttaggtttta
    19261 ctttaaaagg gatttttttc caccagctta aaactggaat tacgcttagt tgcagtagat
    19321 ctctagttaa taccaaataa acataaaact ctaaaactga attgcgaaag agtaggtgca
    19381 aaaaaaatgc aatgactact aatttgtccg caatagggtc tagaactgct cctagacttg
    19441 aagtttgatt gagttttcgg gcaagaaatc catctaaaaa atctgttatc agtaaaaagc
    19501 caataaacac aacagatagg attaaatcta ccttttctat aaaatagata gagggaagaa
    19561 tcaataaaat tctagataaa gttaaaagat ttggtagcaa gaaaatttct ttcatagtat
    19621 ttgcacaatc tagaaatttg gcgaatcaaa gcaaatactt ttaatagagg gaataagaag
    19681 agttttttta ttcaggtttt taaaatggaa gttcttctag gtttttctcc tattcgtcca
    19741 gatgagaagg ttcagaaaga ttaagaacgt ttttctaagc ctctgtagaa tacttcgaat
    19801 ataaaaatag agtaatacaa agtgttgctt acttattttg aaaaaaggaa aaaataagta
    19861 atagaaaatt gtaaatgatt gcgaaactca ctttgtttta cagaacaaat actttcctca
    19921 aaaaaaatta caaattagta tcagctaatg cctgttatcc ttagctagat tctatgagag
    19981 tgaatgagta tgagtttaat cagaattagc tatacttcga aaaaacaaat tgaacttgac
    20041 gaaagatata cccatcacaa cactgaaatt gttgtgcatg ctgtcttgaa attaattcta
    20101 ggtttgatgg taagtgtgag tgttcttgcg caaagtctaa tctacgattc tggtctttgt
    20161 tatatgcgca catacagaca gatttgcact tgcaatcata attcaaaaaa agaaattcac
    20221 agacaaacaa ataaatctga ttgtcatgat aaaagcaaat cagtccatat ttgtagttgt
    20281 aaaaaatcaa aaaatccaaa tgaactttcc aatttattaa aacagatatt ttttctatgg
    20341 caaggcaatc aagacttagc tttagaattt ataaaattta attactctac actattaaaa
    20401 gatgttatcc taaatggata tgggctaaca ctgattaaac ccccaagact ttcataaact
    20461 tttccaatgg atttgattta ttcactgatt aaatcaaatc tatctctatt ttaaataaaa
    20521 caaactgtat atgattacag taaaatatat taaggaaaaa aaatgaaata cgtcataact
    20581 atatctataa tactcatttc actcagttgt aataaatttg cagcaaacaa caatgaggat
    20641 aaaaataaaa atatcgcagc agcaacgatc ctattgaatc aaagaaaaag cacggctgca
    20701 agtgtctctt caataaattt taatgttgtc tctggaacga ctaacgtctc ttgctcgggg
    20761 agcattgcag ggactattgc aggagcaaca aatgcaaaat tagttgattt gaagttttat
    20821 gtccatgata taaaattaat ttcaagtgat ggcagtaagg tggacttttc aattacaacg
    20881 gataataaat ggcaaatggc tgctggctct aatgcctttg gttcttatcc aggagtagcc
    20941 ttattagact ttgaggatgc aactggaaat tgttctagtg ggacaacaga aacgaataaa
    21001 actatttatg gaacttcagc agttgggaat tatgtgggag ttgagtttaa ggtaggagtt
    21061 cctttttatt tgaatcatat gaatgtttct actgcggctt cacctctaaa tatttctgct
    21121 atgtattggg cttgggcaag tggttataaa tttgcgaaga tagaattttt agcaaattca
    21181 aatacaaata tgtttcattt ggggagtgga tcgtgctctg gaaattctac tggtcctgtt
    21241 aatccatgtg gattgcctaa tcgtccaacc atttctttaa ctaaaacaag tggtggattt
    21301 gatgcaacga gagatacaat tactttagat ttaaatactc tttacaatgg agctgatgcg
    21361 tcaggcaccg gcataaccaa ttgtatggct ggaaatggaa tcgctgcttg tcaaccgatt
    21421 ataacgaata tcggtattac tgtatcgaca ggggcttctt caggattgca aacagctttt
    21481 agcctgaaat aaaaaaactt tgtaagagaa gtcaaaaact tctcttacaa ttgtaaggaa
    21541 ttcaaatatg agaaccagta aaaaatacat cctaaatatc ataaaaatat acgttatgtc
    21601 tctgagtatt gtatctattt ctttttgtca gaatttaact cattcagatt caaataaggt
    21661 aaatgatcaa aatacaaaat taactttatc ttatcttgcc atatctagat cgagtgcaag
    21721 ttatacatgg gatttacctg caacttttcc aactccaagg gttcctagtg aaaatccaat
    21781 gtcgaatgcg aaagtggagt tgggcagatt tttattttac gaaaaaaaac tttctcagaa
    21841 tcaaacacaa tcatgcggta gttgccatct acaggaattt gccttttctg atagaaaacc
    21901 atttggagtg ggatcgactg gagaatttca tccaagaaat gcacaacatt taggaaatgt
    21961 ggcttataat accaaagtga cttgggcaaa tacaaatatg ggatcacttg aagtgcaagc
    22021 aagagctcca atgtttggaa tttctcctat tgagctggga ttgacaaatg atacttattt
    22081 agatcgattg aaagcggata ctaattatag gaatttattt tcaaaggcat ttggaggagg
    22141 aacagaaaat attacagagc aaaatgttcg ctttgcaata tctagttttc aaagaagttt
    22201 aatcacaggt agatctcctg ttgataaata tttaaattat ggagataaaa cagcattatc
    22261 cgcatctgct attcggggaa tgtctatttt taatggagag acagcagaat gttttcactg
    22321 tcatggaggt tttaattaca caaatacagt gaatcataca ggcacagttt ttgaggaagt
    22381 atcttatatg agtaatggaa ttatctcaga tgccacctac gcaaccttac cgagtaataa
    22441 aaaaggactt tatgatctaa ctggacttgc gacagacatt ggcagattta aagttccatc
    22501 tcttcgaaat gttgctttga catatcctta tatgcatgac ggtagtatta cttgcacagt
    22561 agcaagtgct agtaatgtag atgcttgttc aaccgaagcg ttaggcaaag tgatagatca
    22621 ttatatgtcc ggaggtcaat ctcatcaaaa taaagatccg caaatcagag cttttagttt
    22681 aacaccgcaa gagaaaacag atttaatcag ttttttaaaa gcacttacgg atgatgagtt
    22741 tataaacaat tctaaattta gtgatccatt taaatgaaaa taatccaaac tattttaatt
    22801 attttatgtc tagaaagttg cacgtttggt tcggaaggga atagtcttac tgataaaaaa
    22861 ataattatca caagatttgc tcttcttaga gattctataa agactaacgc atcattgcag
    22921 ttattatttt atgatgatca aggagatgaa aacttaaata catttcaact caatagtgcg
    22981 aatacaagtg atatttatac tgtcacacta gttgatactt caaaaattag ctttgataaa
    23041 atagagttgt atgtaaatac ttcgagtgca actgttttgg aaagcaatac aagtccaccg
    23101 gaagtttttt ttcatgaggc agatagcgga cctactggat ctgcatctgc tactacgaaa
    23161 gtgtatgtaa cggctaatgg ttatgcaaca tctacatttt taaatgcaaa tggatctgat
    23221 gaattaggtt cttatagtgc cactgataat tttgtcctga caaatctgcc tcagggaatt
    23281 ttacaaaagg ttacctttca ttttggatct attgttctag taggtagttt aaaaagcaca
    23341 actaaaacta agtcttttac aatccagttt ccctctcgtg atttatcttt ttctaggctt
    23401 tgcagtaatt tactaggttt gaatcaaaat ctgaacttaa aattagatat aagatactct
    23461 gacttattta aagatacaac tacaaatacc tatttaacga tagacaatac taaaatacta
    23521 agagcaattc atgatttgtc tgatccgaat ccaaccatta gtatcactca aaattcaggg
    23581 attttcaatg aaataaataa aaattggaat cttacaacaa taataaaaga acataggtgt
    23641 agcaaatgat ttatagtaga aaatattttt tactagcggc tactatttat tatctgcttg
    23701 taactttatc cctgtattcg catcatactg gatcaggtct tgatacaact caattcaatg
    23761 ttaggttttt aggaaaagaa gaggataagg gcggttttcc acctacttat tttattgtaa
    23821 gcaatgaata cagtcgagga gcacaagaga atcgaaatat agatacgaca aatatgtatg
    23881 gtgaaagtat ctttatgcgc gggcttttta cagtgaatgc taatttcagc tatatttatt
    23941 acgatcaaaa agatcgatcc gatttcgctc gttatggcaa accgtatatt ggatcaaaaa
    24001 tatttccttt ttataaaaca caagcggaga atagtttctt cttttttttg gaaggtcgta
    24061 ttggttttcc aacaagtgca gtgaatcaaa aattcattga tagtaattat tatactgctc
    24121 aaggaaatac tgcctttggt tatttgtgga aaagtatttc tattgtagga aaactaggag
    24181 gggaaatgcc aattagccgc tataccggtg atcgtagcgc agaatatgat gaagtccctt
    24241 acatcttgag acctcctcaa acttttactt ggaatcaaat cgataatatc cagttgaaaa
    24301 aaacaacaaa tgcttcagga tttattacgt atatctataa taatcattta tccttgtttg
    24361 gcggttattt atataggact ccattcaatg gaattcaaaa agatagaacg acaggcgata
    24421 agattccact aatatttaga gaagtgtctt ttgggttaaa ttataaattt agcgagaaat
    24481 acatgttaac cgttacctat agaaatcctc tatatagggg aagggatttt cgtccttatg
    24541 agtcatcgat taatatagct tttacattta atctatgatt gagtgattca tgtctttgat
    24601 ctatatcaat gagataaata t
//
LOCUS       DAOQOS010000082        24242 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_985_length_24242_cov_5.924091, whole genome shotgun sequence.
ACCESSION   DAOQOS010000082 DAOQOS010000000
VERSION     DAOQOS010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 24242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24242
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_985_length_24242_cov_5.924091"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..238
                     /locus_tag="PLF66_16820"
     CDS             <1..238
                     /locus_tag="PLF66_16820"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HMZ65755.1"
                     /translation="MSFIHFVNSHRIRIACKMLEEEKRNILSIAYSVGFNSKSAFNRV
                     FRQFIGNSPREYRKNPHLFEKEKSTLIEKIEPKL"
     gene            350..787
                     /locus_tag="PLF66_16825"
     CDS             350..787
                     /locus_tag="PLF66_16825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015679022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65756.1"
                     /translation="MNALKLCYLFAGVFFLVGLLCGIWKYLGIVKSKNGVAYEYISIL
                     HRASLLYSFACILLAKFVELSSLPEEINFYSALAAISFFGFAQITYFLHAVLKDTENQ
                     FSKPYRIGNWFFPSFLLHLSMVFLILGELGGFMILFWGFIRSL"
     gene            complement(996..1319)
                     /locus_tag="PLF66_16830"
     CDS             complement(996..1319)
                     /locus_tag="PLF66_16830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012389738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HMZ65757.1"
                     /translation="MNIYVGNLNYEVTEGDVRNLFETFGDVTSVKIITDKYTGQSRGI
                     GFIEMSNKQEALAAISDLNEKELKGRALKVNEAQPKKSYDNNGGGNRGGYGGGRDRDR
                     GRRNY"
     gene            complement(2528..3100)
                     /locus_tag="PLF66_16835"
     CDS             complement(2528..3100)
                     /locus_tag="PLF66_16835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65758.1"
                     /translation="MKSEKNEILVPLERPFVEELFARYKDQIGKDFPGYRNHVYRTIT
                     YAMHFLDYAKEYERLVETAFVYHDIGLWTDHKLAYLEPSEAVALADNQKYGWGLDSDA
                     LSGAIHWHHKIFPYVGPHEKVIEACRKADWIDASKGIISKGMRRDFIQKVEASFPNLG
                     FHDSLLRLAKDYGGSTIVGGIQVTVGIVKW"
     gene            complement(3406..4188)
                     /locus_tag="PLF66_16840"
     CDS             complement(3406..4188)
                     /locus_tag="PLF66_16840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65759.1"
                     /translation="MKLIYLFLSLILIQNCTQLNSIPFLDSIIETDQEKKSKENQRLF
                     LVAGLALSNSRPSVSNLGAGRIATCDGTGGKPKSEKNLVSLTVGGSVYTAGIAQVTST
                     NQDAFLYKTTNNNLDFCYYYDNSAADTRGDLLVSQDSFLYVAFSTDGGNASFQASTDA
                     MQKSYGTGGGPRVTYLAKMDLSGNILRSTFIGARLDDGKVNTLTPKTLTTSNGNIIFE
                     GNSAYDGGKASDSIDPGNVCASGSTRRIEFNSDLRKISTSCY"
     gene            4641..5042
                     /locus_tag="PLF66_16845"
     CDS             4641..5042
                     /locus_tag="PLF66_16845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65760.1"
                     /translation="MIKQLIEKQQIFNLTLKFLSFLSNWKITQSLILPITNFLAWINI
                     LLNRPEETKDLEKLAEIWKQLMPPDGQDYFKITNITEQTAYVEIHLHCPLRGTGKVHT
                     CYSFMNYDRELMKKVGASLTVIESQSNSGKP"
     gene            5204..6103
                     /locus_tag="PLF66_16850"
     CDS             5204..6103
                     /locus_tag="PLF66_16850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65761.1"
                     /translation="MLRNIKIPLLFILITVSQTFSCSKPLPVKEGKLLTVSATSTAKD
                     SKDAYNVKYLSDDSIKSWCEGKPDDGIGESIEFTFEKEVRIQSFFIKNGFGDPKYFAQ
                     NNRIHKLKIESDKGSFEEVELEDTNSEKEIVLKKPLDATKINFTIISIRSGSKFKDTC
                     ITELGFNHSSITDIGSMKLCGVDFKLFELEYTTPENNFDISYNSELAGGSGSYLNRDL
                     ACSHPEKFIFDSVQIESKEEGNVFSIEAKFETIPSSVDSSCAPRNVSMNLEKCIDGIK
                     EMKLDGSLCNGVKEGSKVKVFCK"
     gene            6219..6797
                     /locus_tag="PLF66_16855"
     CDS             6219..6797
                     /locus_tag="PLF66_16855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65762.1"
                     /translation="MEKIKQVLTILEKENFKPIIKTETFDDFDSKEIFWTDKNGEECF
                     ILEENIAIENSKIAWLQSSENDSHLLKIYDKNITFTWKPITYNPAFGCLCLLIEWYKE
                     YLIFIYQEKHEIYICSIKENTVKHFHFHGEKIERKGNLISYDTYVNKANDKVRLIQIP
                     ELIELEPIDKSKAEKLDLLPKDLNRSGNFLGL"
     gene            complement(7010..9256)
                     /gene="recQ"
                     /locus_tag="PLF66_16860"
     CDS             complement(7010..9256)
                     /gene="recQ"
                     /locus_tag="PLF66_16860"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR01389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HMZ65763.1"
                     /translation="MEQILAIVKKYWGYPSLRPLQAESIQTVLDKKDSLTVLPTGAGK
                     SLCYQAPALLTEGTTVVISPLIALMKDQVDSLRQNGISAAQLDSTLDLEEKSNLEFAV
                     LEGRISLLFVSPERAVMPEFINILKQIKINSFAVDEAHCVSQWGHDFRPEYRSLGKLK
                     KIFQNVSVHAYTATASERVRVDIVEQLSLTAPEVIVGDFDRKNLIYRIVPRNNLTNQI
                     LDTIERHNKEAGIIYCIRRKDVEELNAILTKRGISCLPYHAGMTSEERTKNQELFISE
                     KVDIMIATVAFGMGIDRSNIRYVIHTGMPKSIEHYQQETGRAGRDGLEAECILFYSGS
                     DSILWKKLLGEPDETLGNLEYLSASYSHIDEMEKYCRMSICRHKYLKNYFGQEYEKNN
                     CEACDVCLDKKQIVEDSQTIARKIISGVVRVNERFGVSHITSILRGENLAKIRDFGHH
                     KLSTYGLLKEYSKEQVQDWIYQLISQNILTQEGNPYPILKLTTHAKAVLKSERSVSLT
                     LLPEKEETKSKKIDSESWEGVDQNLFEMLRMFRKDLATQRKVPPFVIMSDVTLRDLSR
                     VRPSSIEKLNLIYGIGEQKLKDFGKEILAIIHKYCTENQIAKDVVYSSTIRTVEKKST
                     APNGTKALAFELFRENKPLEDVIRETGRAKSTVLEYLADYIEEEHPFDISQYVSTDVY
                     EKVSKAIQLVGRERLKPIYLALNETISYDDIRIVVSHFKGSSFLNFNRLPPMAPITER
                     YRNDDSFE"
     gene            9507..10664
                     /locus_tag="PLF66_16865"
     CDS             9507..10664
                     /locus_tag="PLF66_16865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HMZ65764.1"
                     /translation="MANKEFDIVVYGATGFTGKLVSEYLTLRGIKGWAMAGRSETKLQ
                     EVKDEIGIPKDIPLIVADSSDPNSLKALCERTKVVLTTVGPYQLYGNELVSACANSGT
                     DYLDLNGEPAWAKQMIEAYDGVAKNTGARIVLSSGFDSIPSDLGVWFLQQEAIKKFGK
                     PVPRVKGRVREMRGSASGGTMASAKATMKASFKDPSIVSVLANPFALTPGFNGPEQPS
                     GLIPEFDKEFNVWAAPFIMASVNTKNVHRTNFLLNHPYGKDFVYDEMMLTTLGEAALA
                     LVDAAAKANPFVGKGLKPGDGPTKEERENGMYDIIFTGEYPDGKRIRACVKGDRDPGY
                     GSTSKMISEAALTLLETKTAGGVFTPGAIMAAPLLERLQKYAGVTFTIENM"
     gene            10884..12032
                     /locus_tag="PLF66_16870"
     CDS             10884..12032
                     /locus_tag="PLF66_16870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65765.1"
                     /translation="MTRIFFKILVMLPILLNSFCYRSIINKEGVTPLLQPKNKTGIIV
                     YLNRDIAFNASKMVEIEKGRETYLNLKNDISELISNSGKHLEAETGGNLKFLGIYGFE
                     NATFDEGFALKKQKKKNTIEFSFYEIKEEQNFTLLLSIFTVGIFPLWYERTLKTSIRY
                     WDDEGNVRTLPNTNSIPILKSYGGWLLIPFAFFSKEDTKTLYQYTFDLQMRQSMYKYE
                     LGKYSKADYEIFPLKEKQMIQADAEKFLKDDSVWFMIRNEARSRTERFTFYSKSDNIH
                     TLAATVFTKNISNKPIQYNPNRYFVSDGINKYKLDKALNPDTGEFINQDIELQPGEIK
                     EEILAFYVPHDVELIYLNYSDPRQEINFQTGPVLRFYKNFKKYSFPKN"
     gene            complement(12224..12751)
                     /locus_tag="PLF66_16875"
     CDS             complement(12224..12751)
                     /locus_tag="PLF66_16875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65766.1"
                     /translation="MQLNEQNLKTLKHSKTYVTVLPDSLEILAVARISLKCPPLNYLP
                     IDDYFDLDYDKIAKDFQFTPDAIFEWNRGVSNQALFKKSGFDFYEIDKALFHTLKYIL
                     RYTCKQNSLVVGATTRIVQRYLRKTVGTPWKELGSAKFTNNNYRYPVAYTTRDYLQYL
                     KQNLPEKYKIIFEST"
     gene            13162..13314
                     /locus_tag="PLF66_16880"
     CDS             13162..13314
                     /locus_tag="PLF66_16880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65767.1"
                     /translation="MKKYLYLILILGGLYGFFLNYSNTRLDNESSIFILKALAHSKQE
                     ITKENK"
     gene            13764..14486
                     /locus_tag="PLF66_16885"
     CDS             13764..14486
                     /locus_tag="PLF66_16885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016545523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMZ65768.1"
                     /translation="MKPAEAKTTVFRTKNLEKIYTMGDVSVTALQGIDLECYAGELIV
                     LLGPSGSGKSTLLNILGGLDRPSSGRFWFKDQELTNAEEKDLTEFRRDHVGFVFQFYN
                     LIPSLTAEENVLLVTELCKTPMLAIDALKSVGLEDRKDHFPSQLSGGEQQRVSIARAI
                     AKNPDILLCDEPTGALDFKTGQLVLSAIENVNREFGTTTILITHNASISDMADRIFRV
                     GDGKLLEIIKNKKKKSASEIIW"
     gene            14483..16822
                     /locus_tag="PLF66_16890"
     CDS             14483..16822
                     /locus_tag="PLF66_16890"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HMZ65769.1"
                     /translation="MKTLFKKAIRELYNSISQSITIALVVASGVAVLVAALNTYSSLI
                     KARDKFYITGKLADAFATCKRAPVSLAERLKNVEGVSFVETRIIREALLDRPDEEVSS
                     SAKFVSLPNYINRLHVLKGNFPETDEEALVSIGFAEANLIGPGDSVSAILDGKKVKIR
                     ITGIALSPEFIYAMRGANPLPDDRHFGIFWMKRSALESYFGMQETFNDLVFLFAPDII
                     QSNTIHQIDEILAPFGASGAYSRDKLPSHMFLKDEFRQLKATAWSMPIIFLGVAAFLL
                     HVVASRLVQRQREQIATLKALGYGRFAIGLHYFTILGGIGFAGSVSGIIIGLILGTAW
                     TRLYGEYYRLPNLEFSSDFNYSFLGFCIGMISALFGSQSAVKSVVKLEPAQAMRPPVP
                     IYFGTGVLENILKKFSAQNRMILRTFLFKKKRFLLGSLGISSAILISITGLFWKDAVF
                     YMMDTEFNLVTREDVSIFFFTPVPYRAVQEIENLPEVFRAEGYRIVPVKFRNGARTYE
                     SAIQGYSEDSHLRKILSKENYFIQIPKNGIILNEVIAKKLEVKTGDSILAEVLEGNRP
                     KLNFKVTGVISEILGMGAYTDFHFLHSSLGQEKSLTFISLQSDKNAERKLLEKIKKFP
                     KTYGVFTRSSMLRGLREVMERSVLISALILGIFASVISVGVIYNTMIVSLSEKSWELA
                     SLRILGLTRREVFWILVSELILQIIIAIPIGCVLGYWFSWLLMQTTSTEAFQIPLIIL
                     PATYITSVGIALIASFVSFYILWRKVKTLDLISVLKLRE"
     gene            16824..17717
                     /locus_tag="PLF66_16895"
     CDS             16824..17717
                     /locus_tag="PLF66_16895"
                     /inference="COORDINATES: protein motif:HMM:NF024829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HMZ65770.1"
                     /translation="MKEKLIKTISEIIKPPRIYFFAAGIIFLFLLSLAFRPDPIQVEI
                     KKVHRGTFKKMVEEDGITRVKEKYTVYSPVNGVLRRIHKEAGDIVKEGELLAVIDWDL
                     KREIKSPVSGRILSIYRDSSGPVEMGRPLLDIGDIKNLEVVSEILTSEVVSISVGNEV
                     EITEWGGENIIGKIERIEPSAIKKISALGVEEQRTKVIIKFPVPNAMGEGYKVRCRIT
                     AFSKENELLVPTGALFREKSDWKVFSVSSGKAIKKKVEISDRSREETIVKSGLSEDEE
                     VIVYPGENVKEGVSVRGITNQ"
     gene            17762..19270
                     /locus_tag="PLF66_16900"
     CDS             17762..19270
                     /locus_tag="PLF66_16900"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HMZ65771.1"
                     /translation="MLRPIFLIIHIFYLFYSYPLFSESSFTIETVPNPTKIGKGYLID
                     TTKKLTTKEIQDLNEKLIYLEKETSAEFAVIILSSIGEANPKDFAVTLFNYWGIGKKN
                     KDNGLLLLLVMDQRRWEFETGYGLEGLLPDVVLKRIGEEKLVPMLRLGKTGEGIQNVI
                     IEIENRIKINRLEVNVDQETSKKDENNFVNLKNENWVIIYFPYYYSYIFLYCLVFWSL
                     IFYLKSKKKNIVLLYTESIVNWKVLILGSIPFLLELFYTLTFTGYYTIYPLFKPIYLF
                     ISYSVFFSFLEFFYTIEKGNYSEAFNHYQKIDIPFRSIEMKIFSLLFPLPTLLFVVWC
                     LFKRRKLRNLPSLCNHCKTPMLRLDEKKDDFFLKEGQQIEEKIGSIDYDVWFCESCES
                     VKIYAYEALFTSYSKCPSCKFKTHFLESDKTTVSPTCSSSGEGVRVYACKNCNYRKKE
                     KYTIPARDCSKSSGSSTSYGSSSYRSSSSSSSSSGSSFGGGRSGGGGAGGSW"
     gene            20201..21286
                     /locus_tag="PLF66_16905"
     CDS             20201..21286
                     /locus_tag="PLF66_16905"
                     /inference="COORDINATES: protein motif:HMM:NF022470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2855 family protein"
                     /protein_id="HMZ65772.1"
                     /translation="MNQSIIFKISKDEFTKTEFTQKEFPTLQEGQILLEIYKFAFTAN
                     NITYVNLGESVGYWNFFPEKDNWGVVPVWGFGKVIESKNENIPIGEKFYGYYPTASHV
                     VLNPNNVKENGFVDSSEHRSKLPSLYNYYSNVQNDTLYTEDTENIQLIYRPLFTTAFL
                     IKEMILSNKIYNAKSILLTSASSKTGLALADLLFYNRNKDFSFDIIGLTSERNLNFIK
                     NLEYFQNVFHYKKIDAIPKEDVCIIDFSGNKNILSELNSHFGEKLKFLSLVGMVHWEE
                     KLDKQDSINGTVFFAPTQIKKTMKELGPKNFQKNLSDEYNQFIHKTKDWIQFEEINNA
                     EKFKELYLNILSGKTSPEKGYIVSLNK"
     gene            21306..22124
                     /locus_tag="PLF66_16910"
     CDS             21306..22124
                     /locus_tag="PLF66_16910"
                     /inference="COORDINATES: protein motif:HMM:NF018184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HMZ65773.1"
                     /translation="MKFEYEKRLLNAAKFIEKNFDKEIQLDTISKSSFLSTFHFHRLF
                     KAFYGISPSDYLERIRIENSIYLMKFTQLALKDIAFEVGFKNQETFNRVFKKITLHTP
                     SHFRKNKKSKLEKIKISKPILNLQMEKTIVPKTNYVFIRIHSDKNTLAKAWLKLVDIA
                     LQENNFNANSECIGIWNWNRFVDKKTFDLGIITNRNSHKMNSSYIPESKYLIFTYTGE
                     IKKITEVYSYIYKEFIIKEKIYLKPIPPFEVYLKYPPFYHGNECVTKIYLPIED"
     gene            22182..22694
                     /locus_tag="PLF66_16915"
     CDS             22182..22694
                     /locus_tag="PLF66_16915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65774.1"
                     /translation="MKIILFIILGIASIGSIVLILLPNVKTVTVTKKIPFSKEEIWLK
                     IRNLKNQTNWRKEVEEVKILSESNPESWVERLKSGQEIKLKTIQLEEMSYWEMESFDG
                     SLFDTNWKGKLKSISEKETEISFTESFQMKSIVGKIVFYLFLNLENLINQYIESLEIS
                     LNQGETNEKK"
     gene            22681..23121
                     /locus_tag="PLF66_16920"
     CDS             22681..23121
                     /locus_tag="PLF66_16920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65775.1"
                     /translation="MKKNNTIKKSKTKSWADKYNTRTEIEVKHLEKGFADIPENSEML
                     IATPKVFEDYIKKIKKGKSKTLKEVRQDLAKKYSADYTCPLTTGIFIRIVAEANFEKI
                     TQGVDIEKVAPFWRVIEPKSSLAKKLSFGEDFLIRMRKEEGIKD"
     gene            23154..23390
                     /locus_tag="PLF66_16925"
     CDS             23154..23390
                     /locus_tag="PLF66_16925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65776.1"
                     /translation="MKIFGDFPILPILLGLFFILYVIYSWFDHFKSGRFKRIEKEESI
                     KPRLKNGLEPTATDLNLDVSEKILNHDFNSSDSE"
     gene            23420..23659
                     /locus_tag="PLF66_16930"
     CDS             23420..23659
                     /locus_tag="PLF66_16930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65777.1"
                     /translation="MKNYWIGLGEFKKVEIYLHENEWIECVYDWDGGAHNPTRSRISV
                     KDFLKNIPSEVGRLLSSTDLQELVETLQTHFDFTD"
     gene            23954..>24242
                     /locus_tag="PLF66_16935"
     CDS             23954..>24242
                     /locus_tag="PLF66_16935"
                     /inference="COORDINATES: protein motif:HMM:NF019316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM-DISM domain-containing protein"
                     /protein_id="HMZ65778.1"
                     /translation="MLISRMIFFSLLLSFFIPFHLFSDPVQIEGEEETIYISPHLNYF
                     LDKEKKFSFEEISSEGNLTLFQENKNANTGFGYSNFPIWFYFDYETKTSLDL"
BASE COUNT         8424 a         3911 c         3999 g         7908 t
ORIGIN      
        1 aatgagtttc attcattttg taaatagcca tagaattcga attgcctgta aaatgttaga
       61 ggaggaaaaa agaaatatac tctcaatagc ttattcggtt ggctttaatt caaaatctgc
      121 attcaataga gtgttccgcc aattcatagg aaattcacca agagaatata gaaagaatcc
      181 tcacttattt gaaaaagaaa aatctacttt gatagaaaaa atagaaccta agttatagat
      241 caggtcgatt tatttttctc tctatcgtaa aatgaaagac aataaatctt tcgaaacaac
      301 gcaaggaagt tcgaaagata aatcattttt aaaaattgtg gaggaaaaaa tgaacgcgtt
      361 aaaactatgt tatcttttcg caggagtctt ttttttggta ggacttcttt gcggaatttg
      421 gaaatacttg ggaattgtaa aatcgaaaaa tggtgttgct tatgagtaca ttagcatttt
      481 gcacagggca tctttactct atagctttgc atgtattctg ttagcaaaat ttgtagaact
      541 gagtagtctc cctgaagaaa taaattttta ctcagcactt gctgcgatct cattctttgg
      601 ctttgcacag attacttatt ttcttcatgc agtattaaaa gatacggaaa atcaattctc
      661 caagccatat cggattggga actggttttt tccgtcattt ctacttcatc tttcaatggt
      721 gtttttaatt ttgggtgaat tgggtggatt tatgatttta ttctggggat ttatacgatc
      781 tctttagaca cctgcgaacg tatgaaataa aattagattt ctaaaactac gaggcgcaaa
      841 aaggatttta ttcgagtagc gtaggtataa aaaaagtccc tatctcaaaa atgaaacagg
      901 gactttatag aacgcaaagc aaaaagattt aactttttgc ttttgtgtcg atgcctatct
      961 gaaaataggc aaagcggcta atataaattt accgcttagt agtttctgcg accacggtca
     1021 cggtctcttc cgccaccata accaccacgg tttccaccac cgttgttatc atagcttttc
     1081 tttggttgtg cttcgtttac ttttaaagcg cgacctttta attctttttc atttaggtca
     1141 ctgatagcag ctaacgcttc ttgtttgttt gacatttcga taaatccaat cccacgagat
     1201 tgacctgtgt atttgtcagt gataattttt acagatgtaa catcgccaaa tgtttcaaac
     1261 aaatttctta catcgccttc agtaacttca tagtttaagt tgcctacata aatattcata
     1321 ttaatatcct taattttttt agttttgaga attatagaaa agagaaaatt tttaatgata
     1381 agacaagaaa aatcaaaacc caactgtaaa atctttttgt cttaaaagga aaattgtaag
     1441 agaagaggca ggtaaatgcg aatactcaaa gaaacaatca tcaatgctga aacaaaaatc
     1501 caaaactgtt taccactatt catttcttat atatattgtc aaatcaatta tgggcaagaa
     1561 aacttggaat cccccttagt ccccctttgc aaaagggggc tttgcggttt tgggttgttt
     1621 ggttttataa cggcttatca gtattagccg cttttactta gaaaagatac gaaaactaaa
     1681 aagcaaacct agctctaaaa aatcacacct actccaaccg ccccctttcc caaagggggg
     1741 aaggggggat taaaatttct tctccctctc ttccaccgag ttaatcacat tcgaattatt
     1801 tctctccatc gcaaatttct tagcgaggtc gttgtatttt tttgcgtttg acttgtcgcc
     1861 taactttaga tagacttcct atgatataag ttaaaacatc ataaaatgtt acttcaggag
     1921 attcctgcac tttttccttt ttaccaatat gaataagaaa tggaagtatt ttaattttag
     1981 gaggatgttc tgcattatca catctaacaa taagagaatt attctctttc atccaataaa
     2041 atgagtatct ttagaaatga aaagaatttt aatacagcat tagaattaat gtcttcttta
     2101 atctataaag gtatgataag agaagaaaat acaatcgaat cagaaaggaa ctatatatat
     2161 atttaagaaa gggaggatga tgttcacaaa acaattgatg taatgatagc cacattttgt
     2221 tcctaattca tttgcctccc ttacactctg attccgattt taataatatg aaaaaatatt
     2281 tgaaactaaa agtagtaagt tcttaaacct attcaaaaga gcgttatgtc aaatactctc
     2341 aatcgtaatt ttttcgaatt caccgagtat acgattgtgt ctacttggaa tacttacacg
     2401 atacccgcta tggcttgcat ttaattcact ttagaatttc aaaacttctc acagaaagaa
     2461 tcttttccca tcccaacaac aatagaatct atgagatgat tttacaatta gttaaatcat
     2521 taaccattta ccacttcaca attccgactg ttacctgaat tcctccaact atcgtagacc
     2581 ctccatagtc ttttgccaag cgtaataggg aatcatgaaa tcctaaattc ggaaaagaag
     2641 cttccacttt ttgaataaaa tctcttctca tgcctttgct gataattcct ttcgatgcat
     2701 caatccaatc tgctttgcga caagcctcaa ttactttttc atgcggacct acataaggaa
     2761 aaatcttatg atgccaatga atggctccgc ttaacgcatc agaatccaag ccccaaccat
     2821 acttttgatt gtctgccaga gccaccgctt ccgacggttc caaataagcc aatttatgat
     2881 ccgtccaaag accaatatca tggtaaacaa aagcagtctc tacaagtctt tcatactcct
     2941 ttgcataatc taaaaaatgc atagcataag taattgtgcg atacacatga ttgcggtatc
     3001 ctggaaaatc ttttccaatc tgatctttat atctcgcaaa aagttcttct acaaaaggtc
     3061 gctccaatgg aacgagaatt tcattttttt ccgatttcat ttctttcttc aatcctttta
     3121 aacgggaatt aaaaactccc ccttagttgg atttattgta acataaatcc aagaattcga
     3181 caagagcaaa tcatcagtgt ttattggcta attatcagta tgaatatttt tcctaaattg
     3241 cgatggagat agatttgtct cttttttaaa aactctatta aaatgcgtaa tatcatcaaa
     3301 accaactctc tctaaaattt ctactactgg aagagttgtg tccttgagca agatacaagc
     3361 aatttgtatt cattcttaag tgaggttaca ctgtatttgt cgcttttaat aacacgaagt
     3421 agaaattttt ctcaaatcag aattgaactc tattcttcga gtgcttccag atgcgcaaac
     3481 gtttcctgga tcaatgctat ccgatgcttt tcctccatcg taagcactat ttccctcaaa
     3541 gataatgttt ccattggagg tagtgagagt tttaggcgtt aatgtattta cctttccatc
     3601 atctaacctt gctcctataa aagtggatct taaaatattt ccggataaat ccatttttgc
     3661 caaataagtg actctaggac ccccacctgt tccataggat ttttgcattg catccgtaga
     3721 agcttggaag cttgcatttc ctccatcggt tgaaaatgct acatacaaaa aactatcttg
     3781 agagaccaaa aggtcgcctc tcgtatcagc agcagaattg tcataataat aacaaaagtc
     3841 taaattgttg tttgtagttt tataaagaaa tgcatcctga tttgtagatg tgacttgtgc
     3901 aatccctgcg gtatacacac ttcctcccac agtcagactc actaaatttt tttctgattt
     3961 gggtttgcca ccagttccat cacaggttgc aattcttccc gctcctaagt tagatacaga
     4021 aggtctagaa ttggaaagag ccaaaccagc aactagaaat agtctttgat tttctttgct
     4081 ctttttttcc tgatctgttt cgataatgga atctaaaaaa ggaatgctat ttaactgagt
     4141 gcagttttga attagaatga gagatagaaa tagataaatt agtttcataa gatttacttt
     4201 aatatttctt attagactgg taatatttaa aaaaaatctt tttaggaaat gatgcaaagt
     4261 tctaatgtat tttctttaaa aatacaatcg cttatattct cttatgaata attctaaact
     4321 atagagaata aaagcttact tttttcaaaa attatataga aacactctgc atgaagtgga
     4381 attttttgaa tcccttgcat tttttaagaa caatccttaa taaaaactca gaaagaaatc
     4441 ctttctttgg attttaaacg atagttgtca cactgtaata gattctatac tgagacaata
     4501 gaatttaatc ctattctttc aatacaacaa ccgacggatt caagaatatt tttaatttgt
     4561 gtattttgat tgactctcag caaagtaata aataagtaaa agcatataaa ttcatatttt
     4621 tctttgagga gatattcaat atgattaaac aattaatcga aaagcaacag atttttaacc
     4681 taacacttaa atttctttcc tttctttcaa actggaaaat cactcaaagt ctaattttgc
     4741 caatcacaaa ttttttagcc tggattaata tattattaaa tagaccagag gaaacaaaag
     4801 acctagaaaa attagcagaa atttggaagc agcttatgcc gccagatgga caagactatt
     4861 ttaaaattac aaacattaca gaacaaactg cgtatgttga aattcatctc cattgtccac
     4921 tgagaggtac aggaaaagtt cacacttgtt attcttttat gaattatgat agggaattaa
     4981 tgaaaaaggt aggagcaagt ttaactgtaa ttgaatccca atcaaattca ggaaagcctt
     5041 agcaattaga agagaagaag ataatagaga tgatctgact cctgctcatt tgaatcattc
     5101 atttctataa agcggataac aaaacgtaat ctctaatcaa agtttcacta gtctatttga
     5161 aattatattg actcaatata ggtaaatatt ttgatcttac tttatgctca gaaatatcaa
     5221 aatacctctt ttgtttatcc taatcactgt atcccaaaca ttttcttgtt caaaaccttt
     5281 accagtaaaa gaaggcaaac tacttactgt atctgccact tccactgcta aagattcaaa
     5341 ggatgcatat aacgtaaaat atctctccga cgattcaatc aaaagctggt gcgaaggaaa
     5401 gccggatgat ggaatcggag aatcgattga atttacattc gagaaagaag tgaggattca
     5461 aagtttcttt attaaaaatg gttttggtga tccaaaatac tttgctcaaa ataatcgaat
     5521 tcataaacta aaaattgaat cagataaagg cagttttgaa gaagtagaat tagaagatac
     5581 gaattcggaa aaagaaattg ttttgaaaaa acctttggac gccacgaaaa ttaattttac
     5641 aattataagt atccggtctg gctcaaaatt taaagacacc tgtattactg aattaggttt
     5701 taatcattct agtataacgg atatcggttc gatgaaactt tgtggtgttg attttaaatt
     5761 atttgaatta gaatacacta cacctgaaaa taattttgat atttcttata attcagaatt
     5821 agcaggaggc tctggttcgt atttgaatcg tgatctcgcc tgtagccatc ctgaaaagtt
     5881 catttttgat tcagtgcaaa tagaatctaa agaggaagga aatgtttttt cgattgaggc
     5941 taaatttgaa actattccga gttcagttga ctctagctgt gctccaagaa atgtatcaat
     6001 gaatctagaa aaatgcatag atggaataaa agaaatgaaa ttagatggat ctctttgcaa
     6061 tggagtaaaa gagggatcaa aagtgaaagt gttctgtaag taatggattc aatcacctaa
     6121 tgcattgtga accaaatgaa aaaaatgttc cttcttgatt ttattggttc atttgtattg
     6181 cccaatctta taaaaattct taattcgttc ctaaatcaat ggaaaaaata aaacaagttc
     6241 ttaccatctt agaaaaagaa aattttaaac caatcataaa gaccgaaaca tttgatgatt
     6301 tcgattcaaa ggaaatcttt tggacagata aaaacggaga agaatgtttc attttagaag
     6361 agaatatagc aattgagaat tctaaaattg cttggcttca atcgagtgag aacgatagtc
     6421 atcttttaaa aatttatgat aagaatataa cgtttacttg gaagcctatc acatacaatc
     6481 ctgcgtttgg ctgtctttgt cttttaatag aatggtataa agaatatttg atttttatct
     6541 accaagaaaa acatgagatt tatatttgct ctatcaaaga gaatacagta aaacattttc
     6601 attttcatgg agagaaaatc gaaagaaaag gaaatcttat ctcttatgat acctatgtga
     6661 ataaagcaaa cgacaaagta agactcattc aaatacccga attaatagaa ttagaaccta
     6721 tcgataaatc aaaagcagaa aaattagatc tgttaccaaa agatctaaat cgatccggaa
     6781 attttttagg actgtaactt gcaatccctt tcaaatcaca aagttaccca caagcatttt
     6841 aaaactgact actacgggat tgtgtaaaaa cttaaaaaaa atctaaccgc gaagatcgcg
     6901 aaggtcaaaa tttcctagga aattttcaac ttttcttcgt ggtgaaaatc ttgttcttac
     6961 acaaccccat ttcgggtaga aagacgatgg gcaccgaaaa aagatacaat cattcaaaac
     7021 tatcatcgtt tcggtatcgt tctgttatcg gtgccatcgg tggtaatctg ttaaaattaa
     7081 ggaaagagct gcccttgaaa tgcgacacta cgattcgaat atcatcatac gaaattgttt
     7141 catttaaggc gaggtaaatc ggctttagcc gctcccttcc cacaagttga attgcttttg
     7201 aaactttttc ataaacatcc gtagaaacat actggcttat atcgaatgga tgttcttctt
     7261 ctatgtagtc tgctaagtat tctaagacag tgctttttgc tcggccagtt tctcgaatca
     7321 catcctctaa aggtttattc tctctaaaaa gttcgaaagc aagtgccttt gttccatttg
     7381 gtgcagtgct tttcttctca acagttctta ttgtggagga atatacaaca tcctttgcaa
     7441 tttgattttc agtgcaatac ttatgaatga tcgcaaggat ttcttttcca aagtctttta
     7501 gtttttgttc cccaattcca taaataagat ttaatttctc tatgctagaa ggtcttactc
     7561 ttgaaagatc tcttaacgtt acatcactca taataacaaa aggcggaact ttcctttgag
     7621 tcgctaaatc ctttctaaac attcgtagca tctcaaatag attttgatcc actccttccc
     7681 aggattcaga atctattttc ttagatttgg tttcttcttt ttccggtaga agtgttaaac
     7741 taacgcttct ttcacttttt aaaactgcct ttgcatgagt ggttaatttt aaaatcggat
     7801 aaggatttcc ttcttgtgta agtatatttt gcgaaatgag ttgatagatc caatcctgaa
     7861 cttgctcttt cgaatattcc tttaatagtc cataagtaga taatttatga tgaccaaagt
     7921 ctctaatctt tgctaaattc tctcctcgca aaatgcttgt gatatgacta acgccaaatc
     7981 tctcattgac tcgaaccacg ccagaaataa tttttctcgc aattgtctgc gaatcttcta
     8041 caatctgttt tttgtctaaa catacatcac aggcttcaca attattcttt tcatattctt
     8101 gtccaaaata gttttttaaa tatttatgcc tgcaaatact cattctacag tatttttcca
     8161 tctcatcgat atgagaatag cttgcgctaa gatattcaag attgcctaat gtctcatccg
     8221 gctcacccaa taattttttc cagagaattg agtcacttcc cgaataaaat aaaatacatt
     8281 ccgcttctaa cccatctcta cctgctcttc ctgtttcttg ctggtaatgc tcgatggact
     8341 ttggcattcc tgtatgaatc acataacgaa tattacttct atcaattccc attccaaatg
     8401 cgacagttgc tatcataata tctactttct cagagataaa taactcctga ttctttgttc
     8461 tctcttccga agtcatccct gcatgataag gcaaacaaga gatccctctc tttgttaaga
     8521 ttgcatttag ttcctctaca tcctttctgc gaatacaata gatgatacca gcttccttat
     8581 tatgcctttc gattgtgtct agaatctgat tggtaagatt gtttctagga acaatccgat
     8641 agattaaatt cttccgatca aaatctccaa ctatcacttc tggtgcagta agcgatagtt
     8701 gctctacaat atctactctg accctctcag atgcagttgc cgtataagca tgcactgata
     8761 cattctgaaa tatttttttt aacttaccaa gagaacggta ttcaggacga aaatcatgtc
     8821 cccactgact cacacaatga gcctcatcca ctgcaaaaga atttattttt atttgtttga
     8881 gaatgttaat aaattcaggc atgactgccc tctctggtga aacaaataat aacgatatac
     8941 gaccttctaa aacggcaaac tctaaattag acttttcttc taaatccaaa gtagaatcaa
     9001 gttgggctgc agagattcca ttttgtcgta agctatcaac ctgatctttc atcagtgcaa
     9061 ttagcggtga aattacaacc gtagtccctt ctgtgagcaa tgcaggagct tgatagcaaa
     9121 gcgattttcc agctcccgtt ggcaaaactg taagtgaatc ttttttatct agaacagtct
     9181 gtatcgactc agcttgcaga ggtcggaggg aaggatagcc ccaatatttt tttacaattg
     9241 ctaaaatttg ctccatgatt tactcccatt ttcaccaata ttttaaaaag cctttgggtt
     9301 tatataattt aatgagagta attgcgtaat agttcaatga ctttcaataa aaagagaatg
     9361 ggttcggtaa atcattgttt acctcttttt gaatcctttt ttcaattttt acaggaagtt
     9421 taaatagaaa atcaatcatt tattctatac ataatatcta tcaaaaaata ttttgagaaa
     9481 atcaaatttg taaataggat ttatctatgg ctaataaaga attcgacatt gtagtgtatg
     9541 gagcaactgg ctttactggc aaactagttt ctgaatatct cactctacgt ggcataaagg
     9601 gatgggcaat ggctgggaga agtgaaacaa aactccaaga agtaaaagat gaaattggga
     9661 taccaaaaga tataccactc attgtagcag attcttccga tccaaattca ttgaaagcgt
     9721 tatgtgaaag aacaaaggta gttttaacta ctgtaggacc ttaccaactg tatggaaatg
     9781 aattagtttc tgcctgcgca aattctggaa cagattatct cgatttaaat ggagaaccag
     9841 cttgggcaaa gcaaatgatt gaagcctatg atggagttgc aaaaaataca ggcgcacgta
     9901 tcgtcctatc gtctggattt gattccattc catctgatct aggagtttgg tttttacaac
     9961 aagaggcaat taaaaaattt ggaaaacctg tccctcgtgt aaaaggaaga gttcgcgaaa
    10021 tgagaggatc tgcttctgga ggaactatgg catcggctaa ggcaactatg aaagcatcgt
    10081 ttaaagatcc atcgattgta tcagttctcg caaatccttt tgcacttaca cccgggttta
    10141 atggaccaga acaaccatcc ggattaattc ctgagtttga taaggagttt aacgtatggg
    10201 cggcaccttt tataatggcg agtgtaaata ctaaaaatgt tcatagaaca aactttttat
    10261 taaatcatcc gtatggaaaa gatttcgttt atgatgaaat gatgcttaca acactcgggg
    10321 aagcggctct tgcgttagtg gatgcagctg caaaagcaaa tccatttgta gggaaaggtt
    10381 taaagccagg agatggacct acaaaagagg aaagagaaaa tggaatgtat gatataatct
    10441 tcacaggaga atacccagat ggaaaaagaa ttcgtgcttg tgtcaaagga gatagagatc
    10501 ctggatatgg ttcaactagt aaaatgattt cagaagcagc actcacactc cttgaaacga
    10561 aaacggcagg cggtgtattt actcccggtg caatcatggc tgccccactt cttgaaagat
    10621 tacagaaata tgcaggagtc acattcacaa tcgagaacat gtaattaaat ttctcctaga
    10681 ctgtgtaaaa gcttaaaata aatctaacca cgaagatcgc gaaagtcaaa atttcctttg
    10741 aaatatacaa tttttcttcg tggccttcgt gcacttcgtg gtgaaaatct ttcttttaca
    10801 aactcactct aaactttttc cgataattta ttttgaatgt tttcttattt ttatgattcg
    10861 aataatagga caggagaagt aaaatgacaa gaatcttttt taaaatacta gttatgctac
    10921 cgatacttct gaattctttt tgctatcgta gcataattaa caaagaagga gtaactcccc
    10981 tattacaacc taaaaataaa actggaatca ttgtttacct gaatcgagat attgcattta
    11041 acgcaagtaa aatggtagaa attgaaaagg gaagagagac ctatttaaat ttgaaaaatg
    11101 atataagtga attaatttca aactcaggaa aacatttgga agcagaaacg ggagggaatc
    11161 taaaatttct tggtatttat ggatttgaaa atgctacctt tgatgaagga ttcgctctaa
    11221 aaaaacaaaa gaaaaaaaat acaatcgagt ttagttttta cgaaatcaag gaagagcaaa
    11281 atttcacttt gctactttct atatttacag taggaatttt tcctttatgg tatgaaagaa
    11341 ctttaaaaac atcgattcga tattgggacg atgagggaaa tgtcagaact cttcccaata
    11401 caaatagcat tcctatttta aaaagttatg gaggatggct tctaattcct tttgcttttt
    11461 tctcaaaaga agatacaaaa actttgtatc aatatacatt tgatcttcaa atgcgacagt
    11521 cgatgtataa atatgaattg ggaaaatatt ctaaagcaga ttatgaaatt tttccactca
    11581 aagaaaaaca aatgatccaa gcagatgcag aaaaattttt gaaagatgat agtgtttggt
    11641 ttatgatccg aaatgaagct agaagtagaa cggaaaggtt tacattttat tctaaatcag
    11701 ataatataca tactttagcc gcaactgtgt ttactaagaa catttccaat aagccaattc
    11761 aatacaatcc gaaccggtat ttcgtaagtg atggaattaa taaatacaaa ttagataagg
    11821 cattaaaccc agacacggga gaatttataa atcaggatat tgaacttcag cctggggaaa
    11881 taaaagaaga aatcctagca ttctatgttc cgcatgacgt agagttgatc tatttaaatt
    11941 attcagatcc aagacaggaa attaattttc aaacaggtcc tgtgttacgc ttttataaga
    12001 attttaaaaa atacagcttt ccaaaaaatt aaaatagatt cgattatagt gactagaggt
    12061 ataataccaa atggcaaacg taatttcaaa gagagattta attgattcgc atttggcata
    12121 ttgcgcatag aaaagtgcta gaaaactaag atttgtttag agcaaatcca tctttttatt
    12181 ttaaaaatag gacttatcta ttgaggcttg ctataagaaa taattaggta gattcaaaaa
    12241 taattttata tttctctggc agattttgtt ttagatactg aagataatct ctagtagtat
    12301 aggcaacagg ataacgatag ttgttattgg taaattttgc actgcctagt tctttccaag
    12361 gagttcctac agtctttctc aaatatctct gaacaattcg agtagtagca cctacaacaa
    12421 gactattttg tttacaagta tatcgcaaaa tgtattttaa tgtatgaaat aaagctttgt
    12481 ctatctcata aaaatcaaat cctgattttt taaatagagc ctgatttgaa acccctcgat
    12541 tccattcaaa aattgcatct ggagtaaatt gaaagtcttt tgcaattttg tcataatcca
    12601 aatcaaaata atcatcgata ggtaaataat ttaacggtgg acatttcaat gaaattcttg
    12661 caactgctaa tatttctaaa ctatctggta agactgtcac ataggttttt gagtgcttga
    12721 gtgttttcag attttgctca tttagttgca aatacagagg atcttctaag aaattttcat
    12781 tctctttttc tggaaagaaa actcgaaagg caagtctgga tagttattca attctatctt
    12841 cccctaattc ataaatagca aacttatcaa atttttctat caaaattgat tttggtatag
    12901 atagaatcat cttttctttt ctcctactga atcaaatctc agttccatat ttttaaaatt
    12961 ggttttatag tagaacattt tttacagaat gtagaagtta gagaataaaa agcactattt
    13021 ttatttttaa aaaacgattt tacagaatgt tttttagaat ctaattttca tttgaacttt
    13081 tatttagtat aattcttcaa tttaaattgt taaacaaatc cttttaaatc ttttcatacg
    13141 ataaccgata taaaagtaaa tatgaaaaag tatttgtatc taatattgat tcttggtggc
    13201 ttgtatggat tttttctaaa ttattccaat acaagactcg ataacgagtc atcaattttt
    13261 attttaaagg ctctcgctca ttcaaaacag gaaattacaa aagaaaataa atagcgaaat
    13321 tttaccttcc tctttccaat gcttggatct cccttaaaaa aggaatgtat tacctactgc
    13381 ggctattctt tattttccgc aaataggacc atattgttac ccacaagtat gctaaaattg
    13441 actaccaccg ataaagacat tgggcatcga tggttagtat atctaattgt ttttgccatt
    13501 ttacattatt tgtcgattta atcaggttta agcatctaag gcttcaaata ttcctccttt
    13561 aattgtattt aaatgaagta attaaaccga tataggaatc catcggtttt atcgttctat
    13621 tatcggtgcg atgcggcgag cgtagttgaa ccgtgcatcg gcggtactct tttaaaattg
    13681 tgggtaacga tatgaaatag gaaaggataa attagaaata caagtcaatt gacaaaattt
    13741 tgaataaata aaatccctat tatatgaaac cagcagaagc aaaaacaact gtatttagaa
    13801 caaaaaacct agaaaaaatt tatacaatgg gtgatgtttc tgtgactgct ctgcagggaa
    13861 ttgatttaga atgctatgca ggtgaactaa ttgttctact aggaccttct ggaagtggaa
    13921 agtctaccct tctcaatatt ttgggaggac tggatcgacc tagttctggt agattttggt
    13981 ttaaagatca ggaactcaca aatgcagaag aaaaagatct tacagagttt cgcagagatc
    14041 atgttggatt cgtttttcaa ttttacaatt taattccaag cctaacggca gaagaaaatg
    14101 tgcttttagt aactgaactt tgtaagactc ctatgcttgc gattgacgct ttgaaatcag
    14161 ttggtttgga agatcgaaaa gaccattttc cctctcagct ttcaggagga gaacaacaga
    14221 gagtctccat tgcaagggca attgcaaaaa atccagatat acttctttgt gatgaaccta
    14281 ctggggcttt agattttaaa acaggccaat tggtattatc cgcaatcgaa aatgttaata
    14341 gagaatttgg aacaactaca attttaatca cacataacgc ttctatttct gatatggcag
    14401 atcgtatctt tagagttgga gatggaaagc ttttagaaat tataaaaaat aagaaaaaga
    14461 aaagtgcttc ggaaattatc tggtgaaaac tttatttaaa aaagcaataa gagaactcta
    14521 taattctatt tcacaatcta ttacgattgc gcttgtcgtt gcttcgggag tagccgttct
    14581 cgttgctgct ctgaatacct actcttcttt gatcaaggcg cgagataagt tttatattac
    14641 aggaaaactc gcggacgcat ttgcaacttg caaaagagct cctgtctctt tagcggaaag
    14701 acttaagaat gtagagggag tcagttttgt tgaaactaga attataagag aagcacttct
    14761 agatcgtcct gatgaagaag tttcctcttc tgcaaaattt gtaagtctac caaattatat
    14821 aaatcgattg catgtattaa aaggaaattt tccagaaaca gatgaggaag cactagtaag
    14881 tatcggtttt gcagaagcaa atttaatagg acccggagat tcagtttctg caattctaga
    14941 tggtaagaaa gtaaagattc gaatcacagg gattgcactc tcgcctgaat ttatatatgc
    15001 aatgagagga gcaaatcctc ttccagacga taggcacttt ggaatttttt ggatgaagag
    15061 gtctgcctta gaaagttatt ttggaatgca ggaaaccttc aatgatcttg tttttctttt
    15121 tgcacctgat ataatccaat ccaatacaat ccatcaaata gatgagatac tcgcgccctt
    15181 tggagcctct ggtgcatatt caagagataa acttccttct catatgtttt taaaagatga
    15241 atttcgacag cttaaagcta ctgcttggag tatgccgatt atttttttag gagttgctgc
    15301 atttctactt catgttgttg caagtagact tgtgcaaaga caaagagagc agatcgctac
    15361 tctaaaagcg ttaggatatg gacgtttcgc aattggctta cattatttta ccattctagg
    15421 aggtattgga tttgctggaa gtgtctctgg aattataatt ggattaatcc taggaacagc
    15481 atggacaaga ctctatggtg aatattacag acttcccaat ttagaatttt cttctgattt
    15541 taattattct tttttgggat tttgtatcgg gatgatctct gctctctttg gttctcagag
    15601 tgcagttaag agtgtagtca aattagagcc tgcacaagca atgcgtcccc cagtcccaat
    15661 ttattttgga actggagtat tggaaaatat tttgaaaaaa ttttccgctc aaaatagaat
    15721 gatcctaaga acattcctat ttaaaaagaa aagattttta cttggttctc ttggaatctc
    15781 ttctgcaatt ctcatttcta ttaccggtct tttttggaaa gatgcagttt tttatatgat
    15841 ggacactgaa tttaatttag ttacgagaga ggacgttagt atctttttct tcactcctgt
    15901 tccctatcga gcggtgcagg aaattgaaaa tcttccagaa gtatttagag cagaaggtta
    15961 taggattgtt ccagtaaaat ttagaaatgg tgcaagaacc tatgaatccg caattcaagg
    16021 ttattcagaa gacagtcatc tcagaaaaat tctatcgaaa gaaaattact ttatccaaat
    16081 tccaaaaaac ggaattattc taaatgaagt gatcgccaaa aaactagaag taaaaacagg
    16141 agatagtatc cttgcagaag tcctcgaagg gaatagaccc aagttgaatt ttaaagtaac
    16201 aggagtgatt tctgaaattc ttggaatggg tgcctatact gattttcatt ttctacattc
    16261 ctctctaggt caagaaaaga gcctaacgtt tatttcactt caatcagata aaaatgcaga
    16321 aagaaaatta ctagaaaaga ttaaaaaatt tccaaagacc tatggagtat ttactcggtc
    16381 ttcaatgctt cgtggactca gggaagtaat ggaaagatct gtgcttattt ctgctttaat
    16441 tttaggaatt tttgcatcag tgatctctgt cggagtaatt tataatacaa tgattgtatc
    16501 tctttccgaa aagtcttggg agctagcatc tcttcgaatt cttggactca ctagaaggga
    16561 agttttttgg attcttgtaa gtgaactcat tttacaaatt ataattgcaa taccaattgg
    16621 ttgcgttcta ggatattggt tttcttggct gcttatgcaa actacatcta cggaagcatt
    16681 tcaaattcca ctgattattc tacctgcaac ctatatcaca tcagtgggaa ttgcgctcat
    16741 cgcttctttt gtcagttttt acattttatg gagaaaagta aaaactttgg atttaatttc
    16801 tgtattaaaa ttgagggaat aaaatgaaag aaaaattaat caaaactatc tctgaaataa
    16861 tcaaaccacc tcgcatctat ttttttgctg ctggtattat ttttctattt ctcctatcgc
    16921 ttgcgttcag acctgatccg attcaagtag aaattaaaaa agtgcataga ggaactttta
    16981 agaaaatggt agaggaagat ggaattacac gcgtaaaaga aaaatacaca gtgtattctc
    17041 ctgtaaatgg agtgctgaga agaatccaca aagaagctgg agatattgta aaagaaggag
    17101 aactccttgc ggttatcgat tgggatttaa aaagagaaat taaatctcca gtctctggta
    17161 gaattctttc catttataga gacagcagtg gacctgttga aatgggaaga ccccttttgg
    17221 atataggcga tatcaaaaat ctagaagtgg tctctgaaat tttaacttca gaagtagttt
    17281 caatatccgt tggaaatgaa gttgagatta cggaatgggg aggtgaaaat ataattggaa
    17341 aaattgaaag aattgaaccg agtgcaatca agaaaatctc agcccttgga gtagaagaac
    17401 aaagaacgaa agtaattatt aaattcccag tccctaatgc aatgggtgag ggttataaag
    17461 tgagatgtag gattactgct ttttcaaaag aaaatgaact actcgtaccg acaggcgcac
    17521 tatttagaga gaaatcagac tggaaggttt tcagtgtatc ttctggaaaa gcaatcaaaa
    17581 agaaagtaga aatatcagat cgtagtcgag aagaaacaat tgtgaaatca ggcttgtctg
    17641 aagatgaaga agtgattgtc tatccgggtg aaaatgtgaa agaaggagta tcggttcgag
    17701 gaatcacgaa tcaatagtta taatttttat ccgaaaataa acaaacaggg agatcaaatt
    17761 tatgttaaga cctatttttt taataataca tattttctat ttattttatt cctatccctt
    17821 attctcagaa tcttctttta caatcgaaac agttcccaat cctactaaaa tcgggaaagg
    17881 atatctaatt gatactacca aaaagttaac aacaaaagag attcaggatt taaatgagaa
    17941 gcttatttat ttagagaagg aaactagtgc agaatttgcc gttataattc tttcttcgat
    18001 tggagaggca aatccaaaag attttgctgt taccttattt aactattggg gaataggtaa
    18061 aaaaaataaa gacaatggac ttttactgtt acttgttatg gatcaaagaa gatgggagtt
    18121 tgaaactggc tatggtctag aaggactact accagatgta gtattaaaaa gaattggaga
    18181 agaaaagtta gttcctatgc ttcgcttagg aaaaacgggg gaaggtatac aaaatgttat
    18241 tatcgaaata gaaaatagaa taaaaataaa taggttggaa gtaaacgtag atcaagaaac
    18301 ttccaagaag gatgaaaata actttgttaa tttaaagaat gaaaattggg taattattta
    18361 ttttccatat tattatagtt atattttttt atactgttta gtattttgga gtttaatttt
    18421 ttatctaaaa tcaaaaaaga aaaatatagt tctattgtat acagaatcaa tagtgaattg
    18481 gaaagtatta atacttggat caattccttt tttattagaa ttattttata cgttaacttt
    18541 tacaggatat tacactattt acccgttatt taaacctatt tatcttttta tatcctactc
    18601 tgtatttttc tccttcttag aattttttta tactatagaa aaaggcaatt atagtgaggc
    18661 gttcaatcat taccaaaaaa tagatatacc atttcgatcg attgaaatga aaatatttag
    18721 cttacttttt ccccttccga cacttctctt tgttgtttgg tgcctgttta aaagaagaaa
    18781 acttagaaat ttacctagtc tgtgtaatca ttgtaaaact ccaatgctaa gactagatga
    18841 aaaaaaagat gatttctttt taaaagaagg acagcaaatt gaagagaaaa ttggatccat
    18901 tgattatgat gtatggtttt gtgagtcttg cgagagtgta aaaatttacg cctatgaagc
    18961 cttatttact agctattcga aatgtccttc ttgtaaattt aaaacgcatt ttttagaatc
    19021 ggacaaaaca actgtttccc ctacttgttc tagctcagga gaaggagtgc gtgtttatgc
    19081 gtgtaagaat tgtaattacc gcaaaaagga aaaatatacg attcctgctc gtgattgttc
    19141 aaaatcatca ggtagtagca caagttatgg atcttcctcc tatcgttcta gttctagttc
    19201 tagtagttct tcaggttctt cttttggagg aggaagatca ggtggtggtg gagctggtgg
    19261 tagttggtaa tgtatataaa ttatctttta aattataatg atgcatccat aattttttaa
    19321 aatcagatat gcatcgaatt aaaaactgtg tagaaataga attataattt aaaaattcat
    19381 ccttataata atcaaaatcc gcaaaataat tgttaccctc acatctcctt caatagaaaa
    19441 ctttaatctc tttactacaa tcatttcgac taatagagat cataccattg ggtcgctcat
    19501 caattcagtt cctgcaggaa gtctccattt aaaactgccg ctagttgatt ttatacaatt
    19561 tctaatagtt tcctttcatt tgaaataaat tgtagcttta gagtaatcgt tatccaattc
    19621 tcaaattaac agtctcatct agtcagcatt tttgatcttg ataattgact cttataagaa
    19681 tatcaaaatg aaatggtata taatctttac attttttaat tttagcaact aaatttaata
    19741 aatataactc tcctgcctcg ctggtttaaa gatttggtag caaaagttcc catattctct
    19801 ctagctctac ttttataagg ctaagaatga tttggcgcta tattctaaaa gttatattat
    19861 taaaaaaaga atctcctgat aaattctatt taaatacctt tttcttatat agctaatctg
    19921 aaacccgttt acaaattgag tgcatacgca aacaatccga cctcttttca aaatctagtg
    19981 atcacccaaa cctctttacc gtttgatatt ttagaaatca agacttgaat tggtcatact
    20041 cccagttcct aatcctagcc tattcatagt tacataaata gaatagaaac atataagaaa
    20101 atattaagat tgtccctgaa aaggagttaa acagtataga actatttaaa ttagtttcaa
    20161 ctgaatcttt atatttaaac taaagaatag gagcccagcc atgaatcaaa gtatcatatt
    20221 taaaatttca aaagatgaat ttacaaaaac tgaatttact caaaaagaat ttccaacttt
    20281 gcaagaaggt cagatacttt tagaaatata taaattcgca tttactgcaa ataatataac
    20341 atacgtaaat ttaggagaga gtgtcggcta ctggaatttt tttccagaaa aagacaattg
    20401 gggagttgtt ccagtttggg gatttggaaa agtaatagaa tccaaaaatg aaaacattcc
    20461 tattggcgaa aaattttatg gatactaccc tactgcatct cacgttgtat taaacccaaa
    20521 caatgtaaaa gaaaatggat ttgtagattc tagcgaacat agatctaaat taccatctct
    20581 ctataattat tattcaaatg ttcaaaatga tacgttatac acagaggata cagaaaatat
    20641 tcagcttatc tacagacctc tctttactac agcatttctg atcaaggaaa tgatcttatc
    20701 taacaagata tacaatgcaa aatctattct tctcacaagt gcctctagta aaacaggtct
    20761 tgctttggcg gatttgcttt tctacaatcg aaataaagat ttttcctttg atataattgg
    20821 acttacttct gaacgaaatt tgaattttat caaaaatcta gaatactttc aaaatgtatt
    20881 tcattataaa aaaattgatg ccattcctaa agaggatgta tgtataatag atttttctgg
    20941 aaataaaaat atcctctccg aattgaattc tcattttgga gaaaagttaa aatttttaag
    21001 tttagtagga atggtgcact gggaagagaa actagacaaa caggattcta tcaatggaac
    21061 tgtttttttt gcaccaaccc aaataaaaaa aacgatgaaa gaattgggtc caaagaattt
    21121 tcagaaaaac ctatcagatg aatacaatca atttattcac aaaacaaaag actggattca
    21181 atttgaagaa ataaacaatg cagaaaaatt caaagaacta tatttgaata tactatcagg
    21241 aaagacaagt ccagaaaagg gatatattgt ttctctaaac aagtagaacg ttattcgata
    21301 taaaaatgaa gtttgaatat gagaaaagac tgctaaatgc agctaaattt atagaaaaga
    21361 attttgataa agaaatacag ctagatacaa tttcaaaatc tagctttctt tctacttttc
    21421 attttcatag attattcaag gctttttatg gaatctcacc ttctgattat ttagaaagaa
    21481 tacgaattga aaattcaatt tatttaatga aattcactca actcgccttg aaagacattg
    21541 cgtttgaagt gggatttaaa aaccaagaaa cattcaaccg agtatttaaa aagatcactc
    21601 ttcatacacc aagtcatttt agaaaaaaca aaaaaagcaa attagaaaaa ataaaaatct
    21661 caaaaccgat tctaaatctt caaatggaaa agacgatagt tccaaaaaca aattatgtat
    21721 ttataagaat ccattctgat aaaaacactc tagctaaagc ttggttaaag ttagtcgaca
    21781 ttgctttgca agaaaataat tttaatgcta attcagaatg cattgggatt tggaattgga
    21841 atcgttttgt tgataaaaaa acatttgatc taggaatcat tacaaaccgc aactcccata
    21901 aaatgaattc atcttatatc ccagaatcta aatatttaat ttttacctat acaggagaaa
    21961 taaaaaaaat aacagaagtg tattcctata tatacaaaga atttattata aaagaaaaaa
    22021 tctatctaaa gccaattcct cctttcgaag tgtatttaaa ataccctcca ttctaccatg
    22081 gaaatgagtg tgtcacaaaa atttacttac caatcgaaga ttagcaagaa tcgtcaaaaa
    22141 cattcctcga atccatgtta taaaataaga aaggagtcaa tatgaaaatc attctattta
    22201 taatattggg tattgcatct ataggaagta tagtcctcat tctacttcca aatgtaaaaa
    22261 cagtaactgt cacaaagaaa attccattta gcaaagagga gatttggttg aaaattcgta
    22321 atttaaaaaa ccaaacaaat tggagaaagg aagtagaaga agtaaaaatt ctatccgaat
    22381 caaatccaga atcttgggtg gagagattaa aatcaggaca agaaattaaa ttaaagacaa
    22441 ttcaattaga agagatgagc tactgggaaa tggaaagctt tgatggcagc ttatttgata
    22501 caaactggaa aggaaaatta aaatctattt cggaaaaaga aacagaaatt agcttcactg
    22561 aatcttttca aatgaaaagt attgtaggga agatagtctt ttatttattt ttaaatttgg
    22621 aaaatctaat caatcaatat attgaaagtc tagaaatttc actaaaccaa ggagagacaa
    22681 atgaaaaaaa ataacacaat caagaagtcc aagacaaagt cttgggcgga taaatacaat
    22741 acaaggactg aaattgaagt aaagcattta gaaaaaggtt ttgctgatat tccagaaaat
    22801 tcagagatgt taattgcaac tccaaaggtt tttgaggatt atataaaaaa aattaaaaaa
    22861 ggaaaatcaa aaactctaaa agaagtcaga caagatttag caaaaaaata ttctgcagat
    22921 tatacttgtc cactcacgac tggtatattt attcgaattg tagcggaagc aaatttcgag
    22981 aagataacac aaggtgtaga tatagaaaaa gtagcacctt tttggagagt aattgaacct
    23041 aaatcaagtc ttgctaaaaa actttctttc ggtgaggatt ttttaattcg tatgagaaaa
    23101 gaagaaggaa ttaaggattg aaataatttt aaataaatcc aataacatat tttatgaaga
    23161 tattcggtga tttcccaatt ctccccattc tattaggact attttttatt ctctatgtga
    23221 tttatagttg gtttgatcat tttaaatctg gtagatttaa acgaatcgaa aaagaagaat
    23281 cgataaagcc tagattaaaa aatggattag aacctacagc aacagatcta aacctagatg
    23341 tatcagagaa aattctgaat cacgatttta attcctctga ttcagaatag agaataacgt
    23401 atgagatctt tttttttaaa tgaaaaatta ctggattggt cttggtgaat tcaaaaaggt
    23461 tgaaatctat ttacacgaga atgaatggat tgaatgtgtg tatgattggg atgggggagc
    23521 acacaatcca actcgaagta gaatttcagt aaaagatttt ttaaaaaata ttccttcgga
    23581 agtaggaaga ctcctttcat caactgattt acaagaattg gtagaaacat tacaaactca
    23641 ttttgatttt actgattaaa tattctttcc ccttatttat ggttactcat tatttgccac
    23701 gaatagtcag aagttcaaag agaaatatct attttgcaat gttttaaaaa tagaaattcc
    23761 ttgcttttat aatcaagtta gaaacaaata aatagataga ataacgctag cgtatagagt
    23821 tcttctcacg caaaagagga tatttcccaa gtctaaaaag aaattagaat aattctttta
    23881 aatgggcaat atgtcgattt actcatcaat attagtttag gaaatgaatt acttggattg
    23941 gtataaatac aaaatgctta taagtcgaat gattttcttt agcctgctcc tttctttttt
    24001 cattcctttc catctattca gtgatcctgt tcaaatagaa ggcgaagaag aaaccatcta
    24061 tatcagtcct catttaaatt attttttaga taaagagaag aaattttcct ttgaggaaat
    24121 ttcttccgaa ggaaatctca ctttatttca ggaaaataaa aatgcgaaca cagggtttgg
    24181 ttattctaat tttccaattt ggttttattt cgactatgaa acgaaaactt ctttagattt
    24241 at
//
LOCUS       DAOQOS010000083        23938 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_997_length_23938_cov_6.639660, whole genome shotgun sequence.
ACCESSION   DAOQOS010000083 DAOQOS010000000
VERSION     DAOQOS010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 23938)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23938)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23938
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_997_length_23938_cov_6.639660"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(242..859)
                     /locus_tag="PLF66_16940"
     CDS             complement(242..859)
                     /locus_tag="PLF66_16940"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HMZ65779.1"
                     /translation="MGGSFWSQLQESIRFELEKIAQNKKYKKTEIVFSENDPFKGFVV
                     IRSGKYKIYNLNPNGKEAILRVMTDGDMAAGPLIFSGAPNYPATLECLEDGYVLFFET
                     NLFKQFLKSHPDFQNHFTTQMMQFMHYLKNKTSSLMLLSLKERLIEYLRENGADKGFI
                     QLKINKNQLALLLNATPESISRTFKTLEEENLIQVDGDSYKLLSI"
     gene            947..2125
                     /locus_tag="PLF66_16945"
     CDS             947..2125
                     /locus_tag="PLF66_16945"
                     /inference="COORDINATES: protein motif:HMM:NF017727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HMZ65780.1"
                     /translation="MSLFKNKQLDPYRFFFPISILGGITGVSLWLLFWVAQTNTISSF
                     KNIYPLKLHITIMTVFFLLPVIKGFVFTAIPRFTGTSLLNQSELLILTSIQLILITIL
                     LFYNDSLIFYTFQSIDFGFLFLFILHRFKISKLKLSSYLYFLTGGFFLGIIGSIAQLT
                     SVYLNQYYLFLIGKDFIFYGMVPCIIFGTGTRIIPMIVNSENPSKRMEWMARAEGQKW
                     NHLVLVIFILSYLIEFIAVFYLDLGILFKGLRFLICVYWIVTFFHILEIKSFQTKLPR
                     LILVACYLFIIGLAGHTFGFKYSAHLAHFYLAGGLTLLILGIMTRVTLSHGGADMRIE
                     KNSNVFYWIIAVILSASIARSFAYISMETMTSHFAYAAILFTLGLLFWSVKIGRFILK
                     "
     gene            2348..2794
                     /locus_tag="PLF66_16950"
     CDS             2348..2794
                     /locus_tag="PLF66_16950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HMZ65781.1"
                     /translation="MKFMIRIITVLVLLGLPFTFCKKEGETADSNPSSQSDDNKGIGP
                     IKKVDLGDIDSAKATKGKQHFETKCSACHKFEEKVVGPPLSGITKRRTPEWIMNMILN
                     PVEMTQKDPTAMELYSTYLVQMTFQNVTEDETREILEYFRQMDNKK"
     gene            2807..4714
                     /gene="nosZ"
                     /locus_tag="PLF66_16955"
     CDS             2807..4714
                     /gene="nosZ"
                     /locus_tag="PLF66_16955"
                     /EC_number="1.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016544621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050304 - nitrous-oxide reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019333 - denitrification pathway [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-dependent nitrous-oxide reductase"
                     /protein_id="HMZ65782.1"
                     /translation="MRNSLKFLISLTILVSILSCGKKQGQAVLASNAAQKVYVAPGEK
                     DEVYLFTSGGFNGQIGVYGIPSGRLFKIIPVFSLHSENSYGYDDETKDMLLTSKGYVA
                     WDDTHHPEISLTGGNHDGRWLFINGNNTPRIARLDLTTFETKEIIEIPNTAGGHGAPF
                     ITENTEYAISTTRFSVPIPQRDIAIEEMGKGNFNGTISMVKVDPKDGKMSLDLQMLVP
                     GFSYDLTRCGKGPSHDWCFFSTYNTEQAYQMIEVGASKNDKDYVMAFNWVKAKACKDA
                     GKAKDFSGTYYHNYQPENAPAIAEKMSGVKLLNPKDCPGVAYFMPTPKSPHGADVDPT
                     GEYIAAGGKLASVIAVHSYSKLLKATEDKANFTGEVMGIPILKYEATLAGEVKKPCLG
                     PLHTEFDNNGYAYTSCFVSSEVVKWKPGTWEVVQHMPAFYSIGHLSIVGGDNKKPYGK
                     YLVAMNKITKDRYLPVGMELPQSAQLWDISGSTAELLLDFPTVGEPHYAQMIPAAMIK
                     DKTKKIFPLEENKHPYGTKSEKDAKIVKNGNQVHIYMTSIRSHFKPDKVEVKKGDVLY
                     FHVTNLEQDFDVPHGFAINGSTLPNLLIMPGQTKTMKWEAKEVGVYPFYCTDFCSALH
                     QEMQQYIRVSP"
     gene            4724..5329
                     /locus_tag="PLF66_16960"
     CDS             4724..5329
                     /locus_tag="PLF66_16960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016549028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65783.1"
                     /translation="MWNKVLTKNNRWLVLLAAVLMIGSYFQPMWYISLDAPQYPEGLS
                     MYIWTNKISGGTQYDLQNINLLNHYVGMKEIVSSSIPELLFMPYVLLYMILGAIITFM
                     FPRVFMIGLGIVNLVLVAIAGLIDFWRWEYNYGHELNPEAAIIVPGMSYQPPLLGCKQ
                     MLNISACSIPHVGAIVLFLAVAILVYIIWVERKLYLESKGV"
     gene            5331..5768
                     /locus_tag="PLF66_16965"
     CDS             5331..5768
                     /locus_tag="PLF66_16965"
                     /inference="COORDINATES: protein motif:HMM:TIGR04401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-helix bundle copper-binding protein"
                     /protein_id="HMZ65784.1"
                     /translation="MNRKELLQTAVIASPILLSGKLFSQDTKSSDSGKTGGKYGALID
                     ATTDCLKKGELCISHCVDMMAGGDKTMGPCAKSVRDMLTMCDTLVKLATSNSEFTKKH
                     ASLCLEVCKKCEDECKKHPKHKVCMNCAESCKACIAEINKIIG"
     gene            5773..6279
                     /locus_tag="PLF66_16970"
     CDS             5773..6279
                     /locus_tag="PLF66_16970"
                     /inference="COORDINATES: protein motif:HMM:NF017394.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase accessory protein NosL"
                     /protein_id="HMZ65785.1"
                     /translation="MRKIIAFLANRELTQFKLVISAIILSLVYSCSESGPESVKPGEH
                     KCEFCKMGIVNLNYNSQILTKKGRRYNFDSIECLFSYWNQNEDKVEKVWVKNYLNSQD
                     WLEVENAKFLKAEKLPSPMAANLSSYRSLEDAEKNLTIYGGSILSREDVMHFIKTNWD
                     KDLSQKSK"
     gene            6287..7537
                     /gene="nosD"
                     /locus_tag="PLF66_16975"
     CDS             6287..7537
                     /gene="nosD"
                     /locus_tag="PLF66_16975"
                     /inference="COORDINATES: protein motif:HMM:TIGR04247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071941 - nitrogen cycle metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase family maturation
                     protein NosD"
                     /protein_id="HMZ65786.1"
                     /translation="MKPNRILFTILYLTLSSCIYDLNAKTLNVCNSCEWKTIQDALNA
                     ATTGDTVEVEEGTFKESPIQIKKSVKLIGINFPTIDGNNKESVIDITADSVEVSGFKI
                     INGGISDIHEFAGIHIENSKNCLIHDNHLENNAYGIYLAKVQNCRIENNDSIGNAVNE
                     VSGGNGVHIWSCENIQVIKNKTSMHRDGIYIEFSNSLVIEENYSTESIRYGLHFMFSN
                     DNKFIKNHFTRNSTGVAIMYSKNIEILENFFEKSKDSNSYGILLKDISDSNFKNNIFQ
                     ENTIGITADNATRNNFIHNTFKKNGWAFNMMGNCDDNQLVENNFIENVFDLSTNSREN
                     RNKYEKNFWDSYNGYDLDKNGFGDKPYLPVRFFSYWVNVYPFLMVLFQSPIIEFLEIA
                     ERAFPVMTPVDLKDNFPQIKKIAL"
     gene            7534..8250
                     /locus_tag="PLF66_16980"
     CDS             7534..8250
                     /locus_tag="PLF66_16980"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMZ65787.1"
                     /translation="MIKISNLSKSFNKNKVIHNLNLEIKEGLITALVGPNGSGKTTLL
                     KCILGLTYPDKESRVLLSGFEYLNKNGIKEIGYMPQTPLFPQNLKVKEIIQILKNLEE
                     KETIYEENLKDELEINKFENKYFGELSGGMKQKVNILQCFSSEKKFYIIDEPTASLDP
                     HISFYLKNLLKERRKKGSSILFTSHIMSEVDEIADRVIVMVEGKLILDETPLEILNKS
                     NTTNMESALRSYWMVGVNHA"
     gene            8243..9037
                     /locus_tag="PLF66_16985"
     CDS             8243..9037
                     /locus_tag="PLF66_16985"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HMZ65788.1"
                     /translation="MRNLIQVAIFEFKENIRNKWIFAYGIFFFLISSLLIYFGGNESA
                     KIIASLLSIILLLVPLFALLFGSMNFLDSMSFMEVIFTRPISRLQYFNGKWFGLSFAL
                     NIGYLLGAGLPILLFTNPESKLIHLSVQLLLYGMLLNWVFISIAILVAITFVKREVVL
                     SMSLAIWFYLYLLYDLIMLGVSINFGEYPLEVPILILTCINPLDLVRIITILQMDNAV
                     LLGFTSAFFQKYLGNTNGIILCITFLNIWAILPFYIGYRVFKKKDL"
     gene            complement(9247..9651)
                     /locus_tag="PLF66_16990"
     CDS             complement(9247..9651)
                     /locus_tag="PLF66_16990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65789.1"
                     /translation="MGQGEIKREHLRLSEADFPELESRTKQLMSVILNLFSCEPIQTI
                     DWPYRYSLTKNQNKYEFIFSQMGSFTLRLAQSERYRRNPPPIFYMSIGKYAEKEFVWE
                     DFNANPLSLLESDLVKVIEESIQHYENSVQEI"
     gene            complement(9618..10376)
                     /gene="mazG"
                     /locus_tag="PLF66_16995"
     CDS             complement(9618..10376)
                     /gene="mazG"
                     /locus_tag="PLF66_16995"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020772206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HMZ65790.1"
                     /translation="MAQLRSENGCNWDKKQTYETLREFIIEESYEVVDAVENKNFPHL
                     REELGDLLFNIVFFARIAEEEKRFNFKDIAIEISEKLIRRHPHVFNVPANLTPDEVLA
                     NWNRIKEEEKKNNPKPVSILDPVPKSLPSILRAEKLQKLAAKVGFDWEKLEDVKSKLQ
                     EEIDELYAEIENPSMDQTKIEDEIGDVLFSVINLARFLKISPEVALNRANLKFTSRFQ
                     FIEKEAKAENKNLEDMTLEEMDKLWDKAKLNENI"
     gene            10518..11057
                     /locus_tag="PLF66_17000"
     CDS             10518..11057
                     /locus_tag="PLF66_17000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002721374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65791.1"
                     /translation="MNRFLFISLLITVAAFSRLLPHPGNFTPVMAIALFSGATILSKR
                     ESLLVPFLAMLISDAIIGFHSLIPVVYACMAVITIMGWYLKDNRSFSRTLGFSLIGAV
                     LFFIVTNFGVWATSGMYEKSLNGLIQCYILAIPFFQNTLASTLIYSLVLFGAILLFEK
                     IQTQSFAKATIPVDNKKRS"
     gene            11150..11905
                     /locus_tag="PLF66_17005"
     CDS             11150..11905
                     /locus_tag="PLF66_17005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004755450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HMZ65792.1"
                     /translation="MILMPIPNHDFDPTETAIPWKILTSKGYQIEFATPDGKLGEADP
                     IMLTGKGLGVWKKILMAREDAIAAYSEMKASKSFQNPKTYNSIRVSDYEGILLPGGHA
                     KRMKDYLESSILQKFVGEFFLTGKPIGAICHGVVLAARSKNPETGKSILFLKKTTALL
                     NSQEMTAYNLTRIWMGNYYLTYPEITVQNEIKSVLSHPSQFIEGPSPIFRDSEKNLDR
                     GFSLRDGNYLSARWPGDAYNFSGAFINMLIEKD"
     gene            11954..12316
                     /locus_tag="PLF66_17010"
     CDS             11954..12316
                     /locus_tag="PLF66_17010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65793.1"
                     /translation="MKKLLFLSILLITSVLINCSTTTKVKQFNNTTTPMGKPKYYQTT
                     TNIAVHFFFGLIPIHDKLGNSSFEQTLDDFTDDAVKNRAPKVHIIQRETTRYWYILLP
                     FTIILTPVSTELTGFVQD"
     gene            complement(12358..12870)
                     /locus_tag="PLF66_17015"
     CDS             complement(12358..12870)
                     /locus_tag="PLF66_17015"
                     /inference="COORDINATES: protein motif:HMM:NF019235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1564 family protein"
                     /protein_id="HMZ65794.1"
                     /translation="MEKIFSNPQISFPCENLKLADFSSTLLIPEKYFSLLQSKINQHK
                     GVRSYVAYLLSKYQIFIANGMIPGYSNVTTKYQDKGQNLHKVAFRPRPEDWAELKLYR
                     VSFGMSISAFLVYLLIADSVELAATLSNYLTGVGISVLPNFNLSAKVFLWNKKTFYTR
                     IFQYRKSQYH"
     gene            13183..13917
                     /locus_tag="PLF66_17020"
     CDS             13183..13917
                     /locus_tag="PLF66_17020"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HMZ65795.1"
                     /translation="MKKIIYLIIILVFIFLNCKKETNQSVDGSSNSVEEKLNFKNRLY
                     ITASSLKIRSEPNLKATSVGTLEKGDSIRITEEGGEEIEVEGNKGKWGKITVGDKTGW
                     IFLGFVSNKPPQLISDAEVERITRNYNREVEKCEIEEKREYEKHKNDPSYQSCAICGC
                     DVKTSDSLNNDKGCKEYKLNEIAELGKQKGEQRLSHSLFQEYNDKYCLESYMNYQSQC
                     SVTELGKEFMRISQIIEDEYMQKNPK"
     gene            13914..14840
                     /locus_tag="PLF66_17025"
     CDS             13914..14840
                     /locus_tag="PLF66_17025"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HMZ65796.1"
                     /translation="MKNILQFILILAIFLSFALSASYFIYKQEKENKLIFVKGGTFTR
                     GRIVTKRPDESPVHRVEVSSFYYQETLVTQRQYKEFTKQSGYITTAETKGACLISVEG
                     MKDWEWFEKKGANYLHPFGIDSEIEVNEDYPVVCLSYLDAKAYCEYYQMRLPTEAEWE
                     YAARAGSKERFPWGESPTFNGKYMLNFWQGEDHKNANSKDGNKYLSKVKAYPPNVWGI
                     YDAVGNVWQFTDDFYDPLTYLQASEMEKETGKPIKDPKGPLDGNKKVTRGGSWWCSEK
                     TCNGYGLYFRGKVFTYSAFNNNGFRCVKDNNN"
     gene            complement(14837..17374)
                     /locus_tag="PLF66_17030"
     CDS             complement(14837..17374)
                     /locus_tag="PLF66_17030"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HMZ65797.1"
                     /translation="MKLRTKITFFTLSTVLTIFLLAMGINTFVFYSQAKESIQKEIHS
                     SVSFFLSEINRATNTTEIFGKDLAQTGEIFYNTKIQEKPKDILPSVITDKMKGFSNIT
                     GGGIWYEPNLLGEKYFGLYGILRNGNVDITWEYSNDSYNYFKQNWYLQALPESWNRNT
                     PRSEKYYRTTPYKDSLGNEQVIFITLCTIMYDKTKKIIGMSTIDWTLKSIKDLLSKLN
                     ITKSSFAVLIDRESKKILFFPNEIYLLKDYRELSWLKNNDFRNIQKGEISKESDIIIQ
                     DQVHSLYITETDARFILVIAVNEKEAYSLMIGIIIRNIILTIVTLLLTGVAVSFIVKR
                     SLSPLMSIIDILRGIASGSKGLKERIQIQSKDEFGELAKTFNQMADTIDRQNLELKNY
                     AETLEDKVKERTEELNRTLQEVTFLKNQQDGDYFLTSLLLKPLGRNRIQSETVKVDFL
                     VKQKKEFVFKNKRNEIGGDLCATDSIQLANKTYSVFLNADAMGKSIQGAGGALILGSV
                     FHAIIERTHSNFDTSQTSPERWLKNTFIELHKTFETFDGSMLVSMILGLVDESNGFCY
                     IMNAEHPLAVLYREGKASFLETKSFYRKLGTPDAQGGIVIDTFLIRPGDILFLGSDGR
                     DDISIGSDASGKKLINEDENLFLNIVENSNGDLNKIQEVLKSKGEVTDDLSLLRLEYT
                     GKTHEVPELSDQEMDLVQSAIQEEKKGHNQKAMKILMEANNGNPNHPAILREMMRVYL
                     NTKSFVKICEIGIPYIQERPEDLDMIYLVSFCLKRLKKYESALDYAERLKMREPNNTV
                     YLIHLAEIYILLSNFSKAEHTINQLKNIDPENEKIFALEEEFEAKRG"
     gene            complement(17515..18372)
                     /locus_tag="PLF66_17035"
     CDS             complement(17515..18372)
                     /locus_tag="PLF66_17035"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004450187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HMZ65798.1"
                     /translation="MNQVCYLCSSSDNHPVFNENGILIVKCNSCSHVFSTYEQEEHYD
                     GYWGEGDQEYDLDWWDHAHRAIYSEFIETFMQKDSGTILDVGCGLGFFIKMLKEKKPN
                     WTSIGFEMSEPAVKFAREKNGLTTVYPGMVQNSGIGKGSVDIITLWDVIEHIPKPQPL
                     LEYLYSILKPGGILFLQTPNVPVQLIKAKLKVALKGMRPDVHYLEAKDHINDYSRKTL
                     SMLAAQTGYFETDYFVLTPILSFAGSKSSFGKIGKMLFYYLTKFVWIVSFKTIMINNT
                     LFAVLKKKS"
     gene            complement(18375..18935)
                     /locus_tag="PLF66_17040"
     CDS             complement(18375..18935)
                     /locus_tag="PLF66_17040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65799.1"
                     /translation="MNYKTLTLLIFLMTSSLISDEMAPFKKYINFNHTGENGEKLNPE
                     DFAGYDELRRWANFQGMSLKPDSGKYGLQDGICRMIPSQGFSFYLTFDTANKKPIYLY
                     LDLTTYDRLNNEIYPIRALTVLINGKTKAKIYFDKNSSGSNPYRITVDPVECPTGRIN
                     VTLIPDSTANGRFWGIWDVFYSYVKE"
     gene            19058..19990
                     /locus_tag="PLF66_17045"
     CDS             19058..19990
                     /locus_tag="PLF66_17045"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HMZ65800.1"
                     /translation="MKVFNDIQEIEGNVPNGSVITLGNFDGIHIGHLSLIKRVKEVSK
                     AKKLPSILVTYFPNPAIVLGKKKNLKSIYTEEIKRELIESFSLDMLLTIPFTHEFSTM
                     HAYEFTKNILVDKLKPHHIIIGFNHFFGKDRQGDFAFLQSHAEEFDYSVEKIDQVYFN
                     GEPVSSSNIRTYIQNGDIAKANSVLTRPVMIRGKVVKGFQRGRQIGFPTANIQLAGES
                     LIPPDGVYSVFVVINQKKYKAMLNIGKNPTFENQDQSIEAHIFDFNEEIYGQTIDCHF
                     IDRIRDEIKFSGIDALKEQLQKDAIAAKQSLQSR"
     gene            complement(19976..21907)
                     /locus_tag="PLF66_17050"
     CDS             complement(19976..21907)
                     /locus_tag="PLF66_17050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65801.1"
                     /translation="MKQVFEKMKGRVNFIILIIRNWIEKLVNSFLQFVQKAFLSIKTK
                     FNHFKEYILTDRKRTILFVLFFPFVASLGTTSYFLLRKHFKVHTSIQSGFGMVPLDAE
                     FYIEIKKTQSLKEKLSKAKFFSEIETSEAWTKFISSLAFKKLGEVLYIVELKSNSLLN
                     WSDFIELFADSIGYISFQDGNYSVIAKTNLKSQFGINVFESFKARNIPLKEAKDKTQE
                     EKKKDNPTSNENSNNSPEGANSENPQEENLSEGSDTSVVSEDNYEKSYLEEGIETGNI
                     LISVVNGNNGAIYFVLVDDYFFVSNSIERLTQTLLAATKPSKYSIQGKKEMEGVMKEY
                     YKEDILGLAYFNLTNTNFTPFLKPISENGESISLFFKTENDLLSADIYVTERKNENTE
                     GSISIEKIQSSIPNDVLLAIYSNSIGIKEYINSLLNPGEAWPELSNMVKLFLKNGKVE
                     EENYFPARAGSAILFHKSELKGGVNQLYPDMSLGYVSTKSDDTFLKSIFKTGKPKKET
                     HLDIPIKNYSRESSEYSPSLYQIDKLNWISSNIENAKDCIAAAKGNKPTLSDLRSSQF
                     ESPWKESPHHIVLNWQRLKEDTRVLFIYGGLKSSLYTEKTIQNDIDPLLETFNWIESI
                     HFSLGAKGSIYGKMKLSAL"
     gene            22095..>23938
                     /locus_tag="PLF66_17055"
     CDS             22095..>23938
                     /locus_tag="PLF66_17055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65802.1"
                     /translation="MKRFLPLFLVLIILNTAWNCQKNSKKLSDPNLFVDGFQNAGGLT
                     LFTLLDPYPVLKRPFQTLNVDDFNTRLDNSLKVSAKEDILGTLRLTQSILLNARPQLQ
                     ALLGQTSNMLARIQRNNPATYSNLQPLLERIRNYSQPVIRNVIPITTSYLLQEYTTKS
                     SLTISNQVTSFANTLGNADSQKWLEDYQDLAVKAIRLNSTFRSSLESATNAFLDPIVS
                     SDKKLKNGLIGTIYGFGEMLFKRSGPSQNKTSETTIKELMVNLEKYFTVGGSGYTSIY
                     STSYSTPVTINSAELGTVIVDIYRNIRQLILPPASPTTKDSSAILVDKLAENLQLLDF
                     AGTTTGIETSLLNLILQDSNGRNRFSDSSSDPISALESLFLTLSIVDSFGYVWNVAQW
                     DRTWVTGSTFGQLNLGDSLWALQSTMQSGTQINFNFIMTSSKNRGRVFKNGTALTGTN
                     GIDFNTAELSLLEAESIGPTLPIESSSTGTDLIYRNTVPWVLNWVKRVTFSGYGPYYN
                     KNKKDGNGNFLAPDGSIARYANGTENRFKPTWQTAKYKVCVSNAATTAANCKDVGMGG
                     YEYPTGGSGCATVPADPAVVAPCTNASNPGTGFTIYEIPKSDSERAVNS"
BASE COUNT         8061 a         4096 c         3847 g         7934 t
ORIGIN      
        1 ttttatattc tttcttttgt acaatcctta ttttaggcga cttgacaaag tcgcactaat
       61 aaagagagtc aaatgaaacc atctaattag gtttgctcct atttctgttc acaaataaga
      121 aaagtttaga gagatataaa aagatagaaa ggaaagaatt tttgaatcca ttatcttata
      181 tttttttaga gagagttatg attcaatttc atgcattaga atacacagtt ctataaatat
      241 tctaaataga taaaagctta taactatctc catcgacttg aattaaattt tcctcttcaa
      301 gtgttttaaa ggttctactg attgattcag gtgttgcatt caaaaggagt gctagttgat
      361 tcttattaat tttgagttga atgaaacctt tatctgctcc attctctctt aaatattcaa
      421 ttaatctttc ttttaaactt aaaagcatca aagaagaagt tttatttttt aagtagtgca
      481 taaactgcat catctgagta gtaaaatggt tctgaaaatc aggatgtgat tttaaaaatt
      541 gcttaaatag atttgtttca aagaaaagaa catagccatc ttctaaacat tccaaagttg
      601 ctggataatt aggagctccg gaaaaaatca aaggaccagc tgccatatct ccatctgtca
      661 ttactctaag aattgcttcc tttccatttg gattcagatt ataaatttta tactttcctg
      721 aacgaataac tacaaagcct ttgaacggat cattttcaga aaatacgatt tcagtttttt
      781 tatatttttt attctgtgca attttttcta gttcgaatcg aatcgattcc tgtaactgac
      841 tccaaaaact tccacccaat tttatttttc ctcttgatat aggtcaattc atacatatga
      901 aacttatgat acaagtattt aatcattcat tctattggaa aaaacaatgt cattattcaa
      961 aaacaaacaa cttgatccat atcgtttctt ttttcccatt tccattttgg gaggaatcac
     1021 tggagtaagt ctttggcttt tattttgggt agcacaaacg aatacaatct cttcatttaa
     1081 aaacatatat ccactaaagc ttcatataac aattatgacc gttttttttc tactccctgt
     1141 gatcaaaggc tttgttttta cagcaattcc aagatttact ggcacatcct tattgaatca
     1201 aagtgaatta ttgatattaa cctcaattca gttaatctta ataacaatct tgctatttta
     1261 taatgactca ctcatatttt atactttcca atccatagat tttggattcc tatttttgtt
     1321 catactacac cggtttaaaa tttcaaagct taaactatct tcttatcttt attttttgac
     1381 cggtggattt tttttaggta taattggatc aattgcccaa ctaacaagcg tatacctcaa
     1441 ccaatactac ctttttctaa ttggtaaaga ctttattttt tatgggatgg ttccctgtat
     1501 tatttttgga acaggaacta gaattattcc aatgatcgta aactcagaaa atccaagtaa
     1561 acgaatggag tggatggctc gcgcagaggg acaaaagtgg aatcatttag tcttagttat
     1621 atttattctt tcctatttaa tagaatttat tgcagtattt tatttagatt tgggtattct
     1681 ttttaaagga ttgcgatttc ttatctgtgt ttattggatc gtaaccttct ttcatattct
     1741 tgaaattaaa tccttccaaa ctaaattacc acgtttgata ctcgttgcat gttatctatt
     1801 tataataggc cttgcaggac atacatttgg atttaaatac tcagcacatt tggcgcattt
     1861 ttatttagca ggcggattaa ccttattaat actaggaatt atgacaagag ttactctttc
     1921 tcatggtggg gctgatatga gaatagagaa aaactctaat gtcttttact ggataatcgc
     1981 tgttatcctt tctgcctcca ttgctagatc ttttgcctat atctcaatgg aaacaatgac
     2041 aagtcatttt gcatatgcag ctatactgtt tacactaggt ttattgttct ggtccgtaaa
     2101 aattggaaga ttcattttaa aatagaccaa agagaacaaa tgtgtaaacc cgtaaactta
     2161 caaagaaact acaagcaatt aaaccattac aagtttgctt tttttcaaaa gattattttt
     2221 tttgagaatc cttctgagta tgatttttat cagtttaaaa aaaaatattt aactcatata
     2281 tttgatatac atcaattact cttttttagt tttatgttac ttttagatta aagattagga
     2341 gttaaatatg aaattcatga tacggattat cacggttttg gttctactag gtcttccatt
     2401 tacattctgt aaaaaagaag gcgaaacagc agattcaaat ccaagcagtc aatcagatga
     2461 taataaggga ataggtccaa ttaagaaagt tgatcttgga gatattgatt cagcgaaggc
     2521 gactaaagga aaacaacact tcgaaacaaa atgtagcgct tgccacaaat ttgaagaaaa
     2581 agtcgttggt ccacctttaa gtggaatcac aaaaagaaga acccctgagt ggattatgaa
     2641 tatgatttta aatccagtcg aaatgacaca aaaagatcca actgcaatgg agctatattc
     2701 tacttatctc gttcaaatga cgtttcaaaa tgtaactgag gatgaaacga gagagatatt
     2761 agaatatttc agacagatgg acaataaaaa ataagaggag aataatatga gaaactcatt
     2821 aaaattttta atctcgctga caatcctagt ttcaatttta agttgtggca agaaacaagg
     2881 acaagcagta ttagcgtcta acgctgctca aaaagtatac gttgctcctg gagaaaaaga
     2941 tgaagtatat ctatttacat caggcgggtt taatggacaa atcggagtat atggaatacc
     3001 ttcaggacgt ttatttaaga taattcctgt tttttctcta cattctgaga atagttacgg
     3061 atatgacgat gaaacaaaag acatgctttt aacatctaaa ggatatgttg cttgggacga
     3121 tactcaccat cctgaaatat ctttaaccgg tgggaaccat gatggtagat ggttatttat
     3181 aaatggtaac aatactccac gtatagctag acttgatcta acaacatttg aaacaaaaga
     3241 gatcattgaa attccaaata ctgccggtgg tcatggtgct ccattcataa cagaaaatac
     3301 tgaatatgca atttctacta ccagattctc agttccaatt cctcagcgtg atatcgcaat
     3361 tgaagaaatg ggaaaaggaa atttcaatgg aacaatttca atggtgaaag ttgatcctaa
     3421 agatggaaag atgagccttg atttgcaaat gcttgttcca ggatttagct atgatttaac
     3481 tagatgcggt aaaggtcctt ctcatgattg gtgtttcttt agcacataca atacagagca
     3541 agcttatcaa atgatagaag ttggtgcttc taaaaatgat aaagactatg ttatggcatt
     3601 caactgggta aaagcaaaag cttgtaaaga tgcaggaaaa gcaaaagatt tttccggcac
     3661 atactatcac aactaccaac ctgaaaatgc tcctgccatt gcagaaaaaa tgtcaggtgt
     3721 aaaattactc aatccaaaag attgtccagg cgttgcttat tttatgccaa ctccaaagag
     3781 tccacatggt gctgacgtag atccaactgg tgaatacatc gcagcaggtg gtaaattagc
     3841 ttctgttatt gcagttcact cttattcgaa attattaaaa gcaacagagg ataaagcaaa
     3901 ctttaccggt gaagtaatgg gaattcctat tttaaagtat gaagcaacgt tagccggcga
     3961 agtaaaaaaa ccttgtctcg gaccactaca tacagagttt gataataacg gttatgccta
     4021 tacatcttgt ttcgtaagtt ctgaagtcgt taagtggaaa cctggaacct gggaagttgt
     4081 gcaacacatg ccagcatttt acagtattgg tcacttaagc attgtaggtg gggataataa
     4141 aaagccgtat ggtaaatatt tagtagcaat gaataaaatt acaaaagaca gatacttacc
     4201 agttggaatg gaacttcccc aaagtgcaca actctgggat atatctggaa gcacagcaga
     4261 attactctta gattttccaa ctgtaggaga gccgcactat gcccaaatga ttcctgcagc
     4321 catgatcaaa gacaagacta aaaaaatatt cccattggaa gaaaacaaac atccatatgg
     4381 aacaaaatct gagaaagatg ctaaaatcgt aaaaaatgga aaccaagttc atatttatat
     4441 gacatcgatc cgatctcact ttaaacctga taaagtagaa gtgaaaaaag gagatgttct
     4501 ttatttccat gttacgaacc tagaacaaga ttttgatgtt ccacacggat ttgcaattaa
     4561 cggttctact cttccaaacc tactcattat gcctggtcag acaaagacta tgaaatggga
     4621 agcaaaagaa gttggagtgt atccattcta ttgcacggat ttctgttctg ctcttcacca
     4681 agaaatgcag cagtatatac gtgttagccc gtaggatcat tctatgtgga ataaagtttt
     4741 aacaaaaaat aataggtggc tagttttact agctgccgtt cttatgatag gatcttactt
     4801 tcaacctatg tggtatatat cgcttgacgc acctcaatac cctgaagggc ttagtatgta
     4861 tatctggaca aacaagataa gtggtgggac tcagtatgat ctacaaaata tcaatctact
     4921 aaatcactac gttggaatga aagaaattgt ttctagttcg attccagaac tcctctttat
     4981 gccctatgta ttattgtata tgattttagg agctattatc acatttatgt ttccgagagt
     5041 ctttatgatt ggtcttggga ttgtaaattt ggttttagta gcaatcgcgg ggctaataga
     5101 cttttggcgc tgggaatata actatggcca tgaattaaat ccagaggcag caattattgt
     5161 tccgggaatg tcctaccaac ccccattact aggatgcaaa caaatgttaa acattagcgc
     5221 ttgtagcatt cctcatgttg gggcaattgt gttgtttctc gctgttgcaa tcttggttta
     5281 cataatttgg gtagaaagaa aactctattt agaaagtaaa ggagtttaat atgaacagaa
     5341 aagaattact tcaaacagcg gttatcgcat ccccaatttt attatccgga aaactttttt
     5401 cgcaagatac aaagtcttct gattcaggaa aaaccggtgg taaatatggc gcattgattg
     5461 atgcaacaac agattgtctc aaaaaaggag aactgtgcat tagtcattgc gtggatatga
     5521 tggctggtgg cgataaaaca atgggacctt gcgcaaaatc agttcgcgat atgctaacga
     5581 tgtgcgatac acttgtgaaa cttgcaacat ctaactcaga gtttaccaaa aaacatgcaa
     5641 gtctatgctt agaagtttgc aagaagtgtg aagatgaatg taaaaagcat ccaaaacaca
     5701 aagtatgtat gaactgcgcg gaaagttgta aagcttgtat tgcagaaata aacaaaatca
     5761 taggatgatt atatgagaaa gataattgca tttttagcta acagggaatt gactcaattt
     5821 aaattagtaa taagtgcaat tattttatct ttagtctatt cttgttcaga atctggtccg
     5881 gaatcggtga agcctggtga gcataaatgt gaattttgta aaatgggaat tgtgaaccta
     5941 aattacaatt ctcaaatcct cacaaaaaaa ggaagaagat ataactttga ttctatagag
     6001 tgtttgtttt cttattggaa tcaaaatgag gataaagtag aaaaagtttg ggttaaaaat
     6061 tatttaaatt cgcaagattg gttagaagtt gaaaacgcaa aatttctgaa agccgaaaag
     6121 cttccttctc caatggcagc gaacctttca tcctatcgtt ctttggaaga tgcagaaaag
     6181 aatcttacga tatacggcgg ctcgattctt tctagggagg atgttatgca ttttataaaa
     6241 acaaattggg acaaagacct ctctcaaaaa agtaagtgat taagttatga aaccaaatag
     6301 aattctattc actatcttat atttaacact ctcaagttgc atctacgact taaacgcaaa
     6361 aacattaaat gtctgtaatt cctgcgagtg gaaaacaatc caagacgcac tcaatgctgc
     6421 taccacagga gatacagttg aagtagagga aggaacattt aaagaaagtc caattcaaat
     6481 taagaaatca gtcaagttaa taggtattaa ttttccaacg atagacggaa acaataaaga
     6541 aagtgttatc gatataactg cagattcagt agaggtaagt ggttttaaaa ttataaacgg
     6601 tggaattagt gacatacacg aatttgccgg aattcatata gagaatagta aaaactgttt
     6661 aattcatgat aatcatctag agaataacgc atatggaatc tatttagcaa aagttcaaaa
     6721 ttgtagaata gaaaataatg actcaatagg aaatgctgtg aatgaagttt caggtggaaa
     6781 cggtgttcat atttggtctt gtgaaaacat tcaggtcatc aaaaacaaaa ccagtatgca
     6841 tagagatgga atttatattg agttttcaaa ctctttagtt attgaggaga attacagcac
     6901 agaaagtatt cgctatggat tacactttat gttttcgaat gataacaagt ttatcaaaaa
     6961 tcatttcaca agaaattcaa caggagtagc aattatgtat tcaaagaaca ttgagatatt
     7021 agaaaatttc tttgaaaaaa gcaaggattc aaactcttat ggaatacttc taaaagacat
     7081 atcagatagc aattttaaaa ataatatttt ccaagaaaat acaattggaa tcacagcaga
     7141 taatgctact cgaaataatt tcattcacaa tactttcaaa aaaaatggat gggcttttaa
     7201 catgatggga aattgtgatg ataatcaact cgttgaaaat aattttattg aaaatgtttt
     7261 tgatttatca acaaactcgc gagagaatag gaataaatat gaaaaaaact tttgggatag
     7321 ttataacgga tacgatttag ataaaaatgg atttggggat aaaccatatt tacccgttcg
     7381 attttttagc tactgggtta atgtttatcc ttttttaatg gttttatttc aatctccaat
     7441 tattgaattt ctagaaatcg ctgagagagc atttcctgtc atgactccag ttgatttaaa
     7501 ggataacttc ccacaaataa agaagattgc tttatgataa aaatttccaa tctatcaaaa
     7561 tcttttaata aaaataaagt cattcacaac cttaatttag aaataaaaga aggtttgatc
     7621 acagcccttg tcggtccgaa tggatcaggt aaaacaactc tactaaaatg tatattagga
     7681 ctaacgtatc ctgataaaga atctagagtg ctactatctg ggtttgagta tttaaataaa
     7741 aatggaatta aagagatagg atacatgcca caaactccct tatttcctca gaatttaaaa
     7801 gtcaaagaaa tcattcagat tttgaaaaat ctagaagaaa aagaaacaat ctacgaagag
     7861 aacttaaaag atgaattaga gataaataaa tttgaaaata aatactttgg tgagttgtcg
     7921 ggcggtatga aacaaaaagt gaatatacta caatgcttta gttccgaaaa aaaattctat
     7981 attattgatg agccaacagc tagcttggat ccacatattt ctttttattt aaaaaatctc
     8041 ttaaaagaaa gacgaaagaa aggcagttcg attttattca cctctcacat aatgtcagaa
     8101 gtagatgaaa tcgcagacag agtaattgtg atggtggaag gtaaactaat attagatgaa
     8161 accccattag agattttaaa taagagtaat acaactaata tggaatctgc tcttcgttct
     8221 tactggatgg taggggttaa tcatgcgtaa cctcatacaa gtcgcaatat ttgagtttaa
     8281 agaaaatatt cgaaataaat ggatatttgc ttatgggatt tttttctttt taatttctag
     8341 tctgcttatt tattttggag gaaatgaaag tgcaaaaatt atcgctagtt tgctaagcat
     8401 tatattgtta ttggttcccc tcttcgctct actttttggt agtatgaatt tcttagattc
     8461 aatgtccttt atggaggtga tttttacaag accgattagc agacttcaat attttaatgg
     8521 aaaatggttt gggctttctt ttgcgttgaa catcggttat ctgctcggag caggtcttcc
     8581 tatactactt tttacaaacc ctgaatcaaa gttaatccat ttatcagttc agctattatt
     8641 atatggaatg cttttaaatt gggtttttat ttcaattgca atcctagttg ctatcacatt
     8701 cgtaaagcga gaagttgtgc tatcaatgtc tcttgcaatt tggttttatt tgtatttgtt
     8761 gtatgatctt atcatgctag gagttagtat aaactttggt gaatacccac ttgaagttcc
     8821 aatactaatc ctcacctgca tcaatccatt ggatcttgtt cgaattatta cgattttaca
     8881 aatggacaat gcagttttac tcggatttac atccgccttt ttccaaaaat atcttggaaa
     8941 tacaaatgga attattctgt gtataacgtt cttaaatatt tgggcaattc ttccatttta
     9001 tataggatat agggtcttta aaaagaaaga cttataactt ttattccgct caattgatca
     9061 ttttcagaag aaaatttgat tttgaaaata ttcgataatg ctaatgaaat agtaaaatca
     9121 atatactgcg atagatcggc tgcagaagag ctcataaaga aaacagtgaa cactcattcc
     9181 gttccttctc tttcgctctg ctcaagccga ttttcatttt tcagtaacaa tgggagttaa
     9241 tgtgagttat atttcttgaa ccgaattttc atagtgctga atactttctt caatcacctt
     9301 aactaaatct gattctaaaa ggctcaaagg atttgcattg aaatcttccc aaacaaattc
     9361 tttttctgca tatttgccaa tactcatata aaaaatagga ggaggatttc tgcgatacct
     9421 ttcactctgt gctagcctta aagtgaatga tcccatttgt gaaaaaataa attcatactt
     9481 attttggttc ttagttagag aataccgata tggccaatct atagtttgaa ttggctcaca
     9541 agaaaataag tttagaataa cactcattag ctgctttgtc cttgactcta attctggaaa
     9601 atccgcctca gaaagtctta aatgttctcg tttaatttcg ccttgtccca taatttatcc
     9661 atttcctcta atgtcatatc ttctaaattt ttattttctg ctttggcttc cttttcaata
     9721 aattgaaaac gagaagtaaa ttttaaattg gctcgattca aagcaacctc tggtgatatc
     9781 tttaaaaatc gtgctagatt aataactgaa aataacacat cacctatttc gtcttctatt
     9841 tttgtctgat ccattgatgg gttttcaatc tctgcatata actcatcaat ttcttcttgt
     9901 aatttacttt tcacatcctc taatttctcc caatcgaacc caacctttgc ggctaacttc
     9961 tgtaattttt ctgcacggag tatggaaggt aaagatttcg gaactggatc taatattgaa
    10021 actggttttg gattattttt cttttcctct tctttaattc gattccagtt tgctaaaact
    10081 tcatctggag ttagattagc tggaacatta aaaacatgtg gatgtcttcg tatcaacttt
    10141 tctgaaattt caattgcaat gtctttaaaa ttgaatcttt tttcttcttc tgcaattcga
    10201 gcaaagaaaa caatattaaa aagtaaatct cccaattctt ctcttaaatg agggaagttc
    10261 ttgttttcga cggcatcaac aacttcatat gattcctcaa ttataaactc tcgtagagtc
    10321 tcatatgttt gtttcttatc ccaattacat ccattttcac ttcgcaattg agccattata
    10381 tctattaaat catctatttc tttcaaatct tttcctcatt ctattcttac tgttttttct
    10441 aattagaacc aatcttcaaa caaaatctga cttgacagtc tctatttttg aaattttcct
    10501 attcatgagg ttatactatg aatagatttc tttttatttc attgctaatt actgttgccg
    10561 catttagtag attacttcca catccaggta actttacacc cgtaatggca attgcacttt
    10621 tttctggagc aacaattctt tctaaaagag agtccctctt agttccattt ttggctatgc
    10681 ttatcagtga tgctattatt ggatttcatt ctctaattcc agtagtttat gcttgtatgg
    10741 ctgtaattac tataatgggt tggtatctta aagataatag aagttttagt agaacgttag
    10801 gttttagctt aataggagcc gtgttgtttt ttattgtaac aaattttgga gtatgggcaa
    10861 cttcaggtat gtatgaaaaa tcattaaatg gtttaattca atgctatata cttgcaattc
    10921 ctttctttca aaatacactt gcgtctacgt taatttattc tttagtttta tttggagcca
    10981 tcttgttgtt tgaaaaaatt caaactcaat cttttgctaa ggctacaatt cctgttgata
    11041 acaagaaaag aagctaatcg aaattatcca ctagtaaatt caactaaatg aaacggcgtc
    11101 ctcaagacgc cgtttttgtt ttcacataca actcaccaag gagtctaata tgattcttat
    11161 gccaattcct aaccatgact ttgatccaac agaaacagcc ataccttgga aaatcttaac
    11221 ttcaaaagga tatcaaatcg aattcgctac accagatggt aagctaggtg aagcagatcc
    11281 aattatgcta acaggaaaag gcttaggagt atggaaaaaa attctaatgg caagagaaga
    11341 tgcaattgct gcttattcag aaatgaaagc atctaaatct ttccaaaatc caaagaccta
    11401 caactctatt cgcgtgtcag attatgaagg cattcttctt cctggcggac atgcaaaacg
    11461 aatgaaagat taccttgaat catccattct tcaaaaattt gtaggtgaat tttttctaac
    11521 aggaaaaccg ataggcgcta tttgtcacgg agttgtttta gctgctcgca gtaaaaatcc
    11581 agaaacgggt aaatccattc tgttcttaaa aaaaacaaca gccttactaa attctcaaga
    11641 gatgactgct tataacttaa ctcgaatctg gatgggaaat tattatctaa cttatccaga
    11701 aattaccgtt cagaatgaaa ttaaatcagt attgtcccat cctagtcaat ttatcgaagg
    11761 tccttcccca atattccgtg attcagaaaa aaatttagat cgaggttttt ctctaagaga
    11821 tggaaattat ctttcggctc gctggccagg agatgcatat aatttttctg gtgcttttat
    11881 caatatgctt atagaaaaag attgaactca tacggaatta atagaatctg cttccaagtt
    11941 caggagattc ttaatgaaaa aacttttatt tctatctatt cttttaatca cttcagtatt
    12001 aataaattgt tcaacaacaa ccaaagtaaa acaatttaat aatacaacta caccaatggg
    12061 aaaaccaaaa tactaccaaa caactactaa tattgcagtg catttctttt tcggtttaat
    12121 cccaattcat gataaactag gtaattcaag ttttgaacaa actttagatg atttcacaga
    12181 tgatgcagtt aaaaatagag cccctaaagt tcatatcatc caaagagaaa ccactcgcta
    12241 ttggtatatc ctattacctt ttacaattat tctcactcct gtgagcactg aactcaccgg
    12301 tttcgtccaa gattaattca cttatatagt agtctttctt tgaaaaattt cgaattttca
    12361 gtgatactga ctttttcgat attgaaaaat tcgtgtgtaa aaagtctttt tattccataa
    12421 gaagactttt gcgctcaagt taaaattcgg gagcactgaa attccgacac ccgttaggta
    12481 attcgagaga gttgctgcta actcaacgga gtctgctatt agtaggtaga ctaaaaaggc
    12541 agagatagac ataccaaaac taacacgata aagttttagt tcagcccaat cttcaggtct
    12601 tggacgaaag gcaactttat gaagattctg ccctttatcc tgatactttg tagttacatt
    12661 tgaataacca ggaatcatac catttgcaat aaaaatttgg tatttagata aaagataggc
    12721 gacatagcta cgaacacctt tatgctgatt tattttactt tgaagtaaag aaaaatattt
    12781 ttctggaatc agaagagtag aactaaaatc agctaatttt aaattctcac acggaaaaga
    12841 aatttgggga ttcgaaaata ttttctccat aaaatgcttc ctcttatttt agatttctac
    12901 tatcgcatta ctttcaatac gaaggttttc taatatatat agggaatcaa aacaagagat
    12961 ctgtgcgcaa aaattctatt ttttttctat tatctaattt tttttttatt gattcacaca
    13021 tcatctccta agagaaactt cttaacaaat atgctagttt cttattcgaa aactttcatt
    13081 taggattcag taaaaatata tttatattga aacggaaaga attttctaat tgtaaaatat
    13141 ttttggatac aagaaggttg taaaaaattt caagaggtaa gaatgaagaa aataatatat
    13201 ctaataataa ttctagtttt catatttttg aattgtaaaa aagaaaccaa tcaaagcgta
    13261 gatggttcga gcaattcagt tgaagagaag ttaaatttca aaaatcgcct ttatataact
    13321 gcaagttctc ttaaaattcg ttccgaaccg aatttaaaag caacgtctgt cggaactcta
    13381 gaaaagggag acagtatccg aatcacagaa gaaggcggag aggaaatcga agtagaaggc
    13441 aacaaaggaa aatggggaaa aattacagtc ggtgataaaa caggttggat atttttaggt
    13501 tttgtaagta ataaacctcc acagctgatc tctgatgcag aagtagaaag aattacacgc
    13561 aattataata gagaagtaga gaaatgcgaa atagaagaaa aaagagaata cgaaaaacat
    13621 aagaatgatc caagttatca aagttgcgca atctgtggat gtgacgtaaa aacctctgac
    13681 tccctaaaca atgacaaagg ctgtaaagag tataagttaa acgaaatagc cgaattaggc
    13741 aaacaaaaag gggaacaaag actcagtcat agtttgtttc aagaatacaa tgataagtat
    13801 tgcctagaat cctatatgaa ctatcagtca cagtgctctg ttacagaatt aggtaaagaa
    13861 tttatgcgaa tatcacaaat aatagaggat gagtatatgc agaagaatcc aaaatgaaaa
    13921 acattttaca attcattttg attctggcca tttttctcag ctttgctctc agtgcttcct
    13981 attttatata caagcaagaa aaagaaaata agttaatctt tgtaaaaggt ggaactttta
    14041 ctagaggcag aattgtaaca aaacgtccag atgagtctcc tgttcataga gtagaagtga
    14101 gtagcttcta ttatcaggag acacttgtca cacaacgaca gtataaagaa tttacaaagc
    14161 agtctggata tatcacaaca gcagaaacaa aaggagcatg tctcatatcg gttgaaggaa
    14221 tgaaggactg ggaatggttt gaaaagaaag gggctaatta tcttcatccc ttcggaatag
    14281 attctgaaat tgaggtaaat gaggattatc ctgtagtttg cttatcttat ttagatgcaa
    14341 aagcctactg tgaatactat caaatgcgac ttccaacaga agcagaatgg gaatacgcag
    14401 cgagggcagg gagcaaagaa agatttcctt ggggcgaaag ccctacattt aacggcaaat
    14461 atatgttaaa cttctggcaa ggagaggacc acaaaaatgc caattcaaaa gatggaaaca
    14521 aatacctctc taaagtaaag gcatatccac ctaacgtttg gggaatttac gatgctgtag
    14581 gaaacgtttg gcaatttaca gatgatttct atgaccctct cacctattta caagcaagtg
    14641 aaatggaaaa agaaacaggc aaacctatca aagatcccaa aggtcctctt gatggaaata
    14701 aaaaagtaac tcgtggtgga tcttggtggt gttctgaaaa aacctgcaat ggttatggac
    14761 tttattttag aggaaaagtt tttacctatt cagcatttaa taataatggc tttcgatgtg
    14821 taaaggataa taacaattaa cctctcttag cttcgaattc ctcttctaat gcaaatattt
    14881 tttcattttc aggatcaata tttttcaatt ggttaattgt atgttctgct ttcgaaaaat
    14941 tagaaagcaa aatataaatt tccgctagat gaattaaata cacagtgttg tttggttctc
    15001 tcattttaag tctttctgca taatctaaag ctgattcata tttcttaagc cttttcaaac
    15061 aaaaagagac taagtaaatc atatccaaat cttctggtct ttcttgaatg taaggaattc
    15121 caatttcaca aatcttgaca aaactcttag tatttaaata tactctcatc atttctcgaa
    15181 gaatggcagg atgattcgga tttccattat ttgcttccat caaaattttc attgcctttt
    15241 gattatgacc tttcttttct tcttgaatag cagactgaac taaatccatt tcttgatcag
    15301 aaagttcagg aacttcatga gtttttccgg tatattcaag acggagaagc gacaaatcat
    15361 cagtaacctc tcctttactt tttaatactt cttgaatctt atttagatct ccatttgagt
    15421 tctctactat atttaaaaat aaattttcat cctcattaat cagttttttt ccggaagcgt
    15481 cacttccaat cgaaatatca tctcttccat ctgaacccaa aaacaatata tccccaggtc
    15541 gaattaaaaa agtatcaatt acaattccac cctgtgcgtc tggagttccc aattttctat
    15601 agaaagattt cgtttctaga aaggaagctt tgccttctct atataaaacg gctaacggat
    15661 gctcagcatt cataatataa caaaatccat tcgattcatc cacaagacca agaatcattg
    15721 aaactaacat agatccatca aatgtttcaa atgttttatg aagttcgata aaagtatttt
    15781 ttagccaacg ttcaggagat gtttgacttg tatcaaaatt agaatgcgtc ctctctataa
    15841 ttgcatgaaa tacagaacca agtattaaag ccccacctgc tccttgaatt gatttgccca
    15901 ttgcatctgc atttaaaaat acggaatagg ttttattcgc caattgaatt gagtcagtag
    15961 cgcaaagatc tcctccaatt tcatttctct tatttttaaa tacaaattct ttcttttgtt
    16021 ttactagaaa atctacttta acagtttctg attgaattct atttcttcca agtggcttta
    16081 aaagaagaga tgttaaaaaa taatctccat cctgttggtt ttttaaaaat gttacttctt
    16141 gaagagttct atttaactcc tctgttcttt ctttaacctt atcctctaac gtctcagcat
    16201 aattttttaa ttctaaattc tgtcgatcta tcgtgtcagc catttgatta aaagtctttg
    16261 ccaattcacc aaactcatct tttgattgga tttgaattcg ttcctttaaa cctttactac
    16321 cactcgcaat tcctctcaat atatcaataa tactcataag aggagataaa cttcttttga
    16381 caataaagga aacagcgaca cctgtaagga gtaacgttac tatagtcaag attatattac
    16441 gaataataat acctatcatc aaggaataag cttccttctc attaacagcg ataaccaata
    16501 tgaatcttgc atcggtctca gtaatatata aagaatgaac ttggtcttga attattatat
    16561 ctgattcctt agagatttct cctttttgaa tatttctgaa atcattattc tttaaccaag
    16621 acaattctct atagtctttt aataaataaa tttcattggg aaaaaaaagg atctttttac
    16681 tctctctgtc aattaaaacc gcaaaggagc tctttgtaat attcaatttt gaaagaagat
    16741 cttttataga tttcaaagtc cagtcgatag tcgacatccc aattattttt tttgttttgt
    16801 catacataat tgtgcagagg gtaataaaga ttacctgctc attgcctaag ctatctttgt
    16861 atggtgttgt tctataatat ttttcagatc taggcgtatt tcgattccaa ctttctggca
    16921 aagcttgcaa ataccaattt tgcttaaaat aattatacga atcgtttgaa tattcccaag
    16981 taatgtctac attaccgttt cttaaaattc catatagccc aaagtatttc tccccaagta
    17041 aatttggttc ataccaaatg ccaccaccgg taatattcga aaaccctttc atcttatctg
    17101 ttatcacaga aggtagaatg tctttgggtt tctcctgaat ttttgtattg taaaaaattt
    17161 ctccagtctg tgctaaatct tttccaaaga tttctgttgt atttgtagct ctattaattt
    17221 ctgagagaaa aaaagatact gaactatgaa tttctttttg tatggattct ttagcttggg
    17281 aataaaaaac aaaagtattg atccccattg cgagcagaaa aatcgttaac actgtgctca
    17341 atgtaaaaaa agtaattttt gttctgagct tcatcttcct ttattaacga acattacaat
    17401 cttctatttc aatcaaaaaa agaatttcta aagatttatt ttaaaaaact aaattactca
    17461 acccgtattc tatctaatct ataaaagcaa tgattgattt gttaagccaa acacttagct
    17521 cttctttttt agaactgcaa ataaggtatt atttatcata attgtcttaa agcttactat
    17581 ccaaacaaat ttggttagat agtaaaagag catctttcca atctttccaa aactactttt
    17641 acttcctgca aaggataata ttggagttaa aacaaaatag tctgtttcaa aatagccagt
    17701 ttgagcagct aacatagata atgtttttcg cgaataatca ttgatatgat ctttagcttc
    17761 tagataatgc acgtcgggtc tcattccctt taaagcaact tttagttttg cttttattag
    17821 ctgcacagga acattcggag tttgtaaaaa taatattcct cctggtttta aaatggaata
    17881 caaatattca agtaaaggct gcggtttcgg tatatgttca attacgtccc acagagtaat
    17941 aatatcaacg gaacctttcc caattccact attctgaacc attccagggt ataccgttgt
    18001 tagcccattc ttctctcttg caaattttac agctggctct gacatctcaa atccaatcga
    18061 agtccagttt ggttttttct cttttaacat ctttataaaa aaaccaagac cacatccaac
    18121 atctaaaatt gttccagaat ctttctgcat aaaggtttca ataaactctg aataaattgc
    18181 tctatgcgca tgatcccacc aatccaaatc atattcctga tcaccctctc cccaataacc
    18241 atcgtaatgc tcttcttgtt catacgttga aaatacatga ctacaagaat tacatttaac
    18301 aatgagtatc ccattctcat taaatactgg atgattatca gaactactac aaagataaca
    18361 tacttgattc atttctattc cttaacgtat gaataaaaca catcccaaat cccccaaaaa
    18421 cgaccgttag ccgtagaatc tggtatgagt gtaacattaa ttcgaccagt tggacattct
    18481 actggatcaa ccgtaatgcg atatggattg gatcctgaac tattcttatc aaagtatatt
    18541 ttagcttttg tctttccatt gattaaaact gttagtgcac ggataggata gatttcatta
    18601 ttgagtcgat cgtaggtagt caggtctaaa taaagataaa ttggtttttt gtttgctgtg
    18661 tcaaaagtta gataaaaaga aaatccttgt gatggaatca ttctacaaat tccatcctgt
    18721 aaaccatact ttcccgaatc tggtttcaaa ctcattcctt gaaagtttgc ccagcgtctc
    18781 aattcatcat agccagcaaa gtcttctgga tttagctttt ccccattctc tcctgtatga
    18841 ttaaaattga tatacttttt aaagggagcc atctcatcag atattagaga agaggtcatt
    18901 aggaatatta gaagagttaa agttttgtag ttcataattg tttttgttct atgaatttct
    18961 atatagatat gcttttatga ttgcattcat agtcaagtcc taaagacata gataaaaaag
    19021 atgagcgata gatttttcat tttttaaaag gcttcttgtg aaagtattca atgatattca
    19081 agaaatagaa ggcaatgttc caaatggttc cgttattact ctaggaaatt tcgatggcat
    19141 tcatattggt cacctctctc tcattaaaag agtcaaagaa gtttccaaag caaagaaact
    19201 cccctctatc ctagtcacct acttccctaa tccagcaatt gttcttggaa agaaaaaaaa
    19261 tctaaaatca atttatacag aagaaatcaa acgggaatta attgaatcct tttcgcttga
    19321 tatgctactt acaattcctt ttactcatga attctctacc atgcatgcat atgaatttac
    19381 aaaaaacatt ttagtagata agcttaagcc acatcatatc ataataggct tcaatcattt
    19441 ctttggaaaa gatagacaag gtgattttgc ttttttacaa tcacatgcag aagaatttga
    19501 ttacagcgta gaaaagatcg atcaagtcta ttttaacgga gaacctgttt ccagttccaa
    19561 tattcgaact tatattcaaa atggagatat tgcaaaggct aacagtgtgt taacccgacc
    19621 agtcatgatt cgtggcaaag tagtgaaagg gtttcagaga ggaagacaga ttggttttcc
    19681 aacagctaat attcagttag ccggagaaag cctaattcct cccgatggag tctattctgt
    19741 atttgtagtt ataaaccaaa aaaaatataa agcaatgtta aacataggaa aaaatcctac
    19801 ctttgaaaat caagaccaat ccattgaagc acatattttt gatttcaatg aagaaattta
    19861 cggacaaaca attgattgtc atttcataga ccgtatccgt gatgaaatta aattcagtgg
    19921 aatcgatgca ctcaaagaac aacttcaaaa agatgcaata gccgcaaagc aatctctaca
    19981 gagcagataa tttcatttta ccgtaaatgg atccctttgc acctaaagag aaatgtatcg
    20041 attcaatcca attaaatgtt tccaacaaag gatctatatc attttgaatt gtcttttcag
    20101 tatagagaga tgattttaaa ccaccataga taaataaaac tctcgtatct tcttttagcc
    20161 tttgccagtt caaaacgata tgatgagggg attccttcca aggtgattca aattgggaag
    20221 aacgcaaatc agatagtgta ggcttatttc ctttagccgc agcaatgcaa tcctttgcat
    20281 tttctatatt ggaggaaatc caattcaatt tatcaatttg gtataagcta ggagaatact
    20341 cgcttgactc tctagaatag ttttttatgg gaatgtctag atgtgtttcc ttttttggtt
    20401 tccctgtttt aaaaatcgat ttcaagaatg tatcgtctga ttttgtgcta acatatccga
    20461 ggctcatatc tggatacagt tgattcactc cccctttcaa ttcagattta tgaaataaaa
    20521 tggcacttcc tgcacgagca ggaaaataat tctcctcttc aacctttcca ttttttagaa
    20581 aaagctttac catattagat aattcaggcc aagcctcccc agggttcaat agggagttaa
    20641 tatattcttt tattccaata gaattcgagt atatagcaag taatacgtca ttcggaatac
    20701 tgctctgaat tttttctatc gatatcgatc cttctgtatt ctcatttttt ctttctgtta
    20761 cataaatatc tgcagacaag agatcattct cagttttaaa aaagagagat atggattctc
    20821 cattttctga aattggcttt aaaaatggag taaagtttgt atttgttaag ttaaaataag
    20881 caagaccaag aatatcttct ttataatatt ctttcataac gccttccatt tcctttttac
    20941 cttgaatcga atactttgat ggtttggttg cagctaaaag ggtctgcgtt agtcgttcaa
    21001 ttgaatttga tacaaagaaa taatcatcca ctaaaacaaa ataaattgca ccattatttc
    21061 cattcactac actaattaat atatttcccg tttcaattcc ctcttctaaa taactttttt
    21121 cataattatc ctcagaaaca acagaagtat cactgccttc tgataaattt tcctcctgcg
    21181 gattctcgct attagctcct tccggtgaat tatttgaatt ttcatttgaa gttggattgt
    21241 cttttttctt ttcttcctgt gttttatctt ttgcttcttt taatggaata tttctagctt
    21301 tgaatgattc aaaaacgtta ataccaaact gagattttag atttgttttt gcaattacag
    21361 aatagtttcc atcttgaaaa gaaatataac caatgctatc tgcaaataat tctataaagt
    21421 ctgaccaatt gagtaaggag tttgatttta actcgactat atataacact tctcctaatt
    21481 ttttaaaagc taaagaagaa ataaattttg tccaagcttc cgaggtttca atctcagaaa
    21541 aaaacttagc tttgcttagt ttttctttaa gactttgcgt ttttttaatt tcaatataaa
    21601 actctgcatc tagaggaacc attccaaatc cagattgaat ggatgtgtgc actttaaaat
    21661 gtttgcgtaa aaggaaataa cttgtagttc ctaagctagc tacaaatgga aaaaacaaaa
    21721 caaataaaat ggttctcttt cgatctgtta aaatatattc tttaaaatga ttgaacttag
    21781 tttttatcga caagaaagct ttctgaacaa attgcaaaaa actatttact aacttttcaa
    21841 tccagtttcg tatgattaaa attataaaat taactcgacc cttcattttt tcaaaaactt
    21901 gtttcattta gtttttcacc ttcttaagtt atacatttta tttaataaat caatagctta
    21961 caaattgcaa tcattcgttt ttattttatt aaatttttac tctcaccaag aatttttaat
    22021 tatgatttga ctttttgtga aattcttgcg atttctggta aatcaaactg atcgaatgac
    22081 tgagtgtaat ttctatgaaa cgttttctcc cccttttttt agttctaatc attctgaata
    22141 ccgcctggaa ctgtcaaaaa aactccaaaa aattatcaga tccaaatcta tttgtagatg
    22201 gatttcaaaa tgctggcgga ctcacacttt ttacactctt agatccctat ccagttctca
    22261 aaagaccatt ccaaacatta aatgtagatg attttaatac gaggcttgat aattctctaa
    22321 aagtttcagc aaaagaagac attctaggaa ctcttcggct aacacaaagt atcctcttaa
    22381 atgctcgtcc gcaattacaa gcacttcttg gacagacatc caatatgcta gcaagaattc
    22441 aaagaaacaa tcctgctact tattctaatc tacaacctct tctagaaaga attcgaaact
    22501 acagtcaacc cgttattcga aatgtaatcc caattacaac ttcatactta ctacaagaat
    22561 atacgactaa gagctctctc acaatcagca atcaagtcac aagttttgca aatactcttg
    22621 gaaatgctga ttctcaaaag tggctagagg attaccaaga cttagcagtt aaggcaatcc
    22681 gtctcaactc cactttccga tcttctttag aaagtgctac taacgctttt ctagacccaa
    22741 tagtaagctc tgataaaaaa ctaaaaaacg ggctgattgg aactatttat ggttttggag
    22801 aaatgctctt caaaagaagt ggtccctcac aaaataaaac ttcagaaacc actatcaaag
    22861 agctaatggt aaatttagaa aaatatttca ctgtcggagg aagcggatat acatctattt
    22921 atagcacaag ctattctact cctgtcacga ttaattcagc cgaattagga accgttattg
    22981 tagatatcta tagaaacata cgtcaactga ttctacctcc cgcttcccca accacaaaag
    23041 actccagcgc tatcttagta gataaacttg ccgaaaattt acagctattg gattttgctg
    23101 gaacaacaac cggcatagaa acatcgcttc taaacttgat tttacaagat tcaaatggta
    23161 ggaatcgttt ttcagattct tcttctgatc caatctctgc gctggaaagt ttatttctaa
    23221 cattaagtat tgtagatagt tttggctatg tttggaatgt tgcacaatgg gataggactt
    23281 gggtcacagg atccacattc gggcagctca atctaggaga tagtctctgg gcgcttcagt
    23341 ctactatgca gtcaggaact caaattaatt tcaactttat tatgacctca agtaaaaata
    23401 gaggaagagt ttttaagaat ggaaccgctc taactgggac taatggcatt gattttaata
    23461 ccgctgaact ttccctactc gaagcagagt ctatcggtcc aactctccca attgaatcct
    23521 ctagtactgg aacggattta atttatagaa atactgtgcc ttgggttctc aactgggtta
    23581 aacgagtaac gttttctgga tacggacctt actataataa aaacaaaaaa gacggcaatg
    23641 gaaatttctt agcaccagat ggaagtatcg ctcgctatgc aaatggaact gaaaaccgtt
    23701 tcaagcctac ttggcaaaca gcaaagtata aagtttgtgt gagcaatgct gcgaccacag
    23761 ccgctaattg taaagatgtt ggaatgggcg gttatgaata tccaacagga ggctctggtt
    23821 gtgcaacggt gcctgctgat ccagcggtag tggctccatg caccaatgca agtaatcctg
    23881 gaacaggctt tactatttat gaaattccaa aatcggattc agaacgtgcc gtgaattc
//
LOCUS       DAOQOS010000084        23814 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_1008_length_23814_cov_6.748811, whole genome shotgun sequence.
ACCESSION   DAOQOS010000084 DAOQOS010000000
VERSION     DAOQOS010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 23814)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23814)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23814
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1008_length_23814_cov_6.748811"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..393)
                     /locus_tag="PLF66_17060"
     CDS             complement(<1..393)
                     /locus_tag="PLF66_17060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c peroxidase"
                     /protein_id="HMZ65803.1"
                     /translation="MKKYLIFSVLILALVNCGPSKESIELQKKAKSAFGILPDKMPGS
                     EKDTPELVSLGEKLYFDTRLSANDTQSCNSCHVVKDKKGGVDNKPTSTGAFGKNGGRN
                     SPTVLNAGFHLAQFWDGRAATLVDQAKGP"
     gene            complement(458..916)
                     /locus_tag="PLF66_17065"
     CDS             complement(458..916)
                     /locus_tag="PLF66_17065"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HMZ65804.1"
                     /translation="MNYKVPKDEIKLRLDEFHNLFRESGFKITPQREEIYKVLLESSS
                     HPSVDLIFEKVKSKFPNISLDTVYRNVESLEQANLVNRIVGIGGKNHYEVNIEPHHHF
                     ICSKCHTIYDFTYAKANTEQINLPQNAIQLGKIESTHLIIKGICKKCNSK"
     gene            1027..1692
                     /locus_tag="PLF66_17070"
     CDS             1027..1692
                     /locus_tag="PLF66_17070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65805.1"
                     /translation="MKYIYLILILFGNLYPIYSNTELEKDLEFIEIKTKLDPAWKVYT
                     KKNFLYLERREPVLVMPEGKTNIPQLKSEADEDKIARMKKYGMKMKPNLVYRFEKRWT
                     VNDTIQSDIIKTEIENKIKSLPEKYKITHLIDEDLSKRGKIFYSPKTKRDKQRVKEYF
                     QEKEKLEKKIPLVPDYHLSKFSLFLVSKKGVSNDYTEVYPESVTGEFIQVEKLLFKYR
                     NKE"
     gene            1735..2871
                     /locus_tag="PLF66_17075"
     CDS             1735..2871
                     /locus_tag="PLF66_17075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65806.1"
                     /translation="MNRFLFFVIFCFSLFCNQCQTTKENLPKDRVPIFFQVSNEHKEF
                     FVDLVKNDYSEAEITTIFQNRKDLARTVCEMIYGQKKYSKDVVTQLISAESFTKEPFK
                     TWGHPVPKLIAGCWNFFKLNDVHPNRDFALLAKLYPTEPKDCFTVGFMQAYLMHNTLG
                     CKNLTMVDLDWKILEGHKQMLDLYHSKSFDSETKIANSLSQLKLGWIARFDSRPMEAE
                     TKTDLNSLCFAEHHKMCTNILLDFQKNFLNLNAVYLRLSALHDTNFKMNTSNTKIIYL
                     SNAIDDIYTNRKQFDFMIKETANALTQNQKAIFVHHAAGRSLFGIYELKKTESGGEIT
                     TICKDKYLTTPVEAITSEYTTHFEKVTINKKFPTCESLLKESSN"
     gene            2868..3710
                     /locus_tag="PLF66_17080"
     CDS             2868..3710
                     /locus_tag="PLF66_17080"
                     /inference="COORDINATES: protein motif:HMM:NF027464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4846 domain-containing protein"
                     /protein_id="HMZ65807.1"
                     /translation="MKQKSSFISIIVFLSFLLNLYCVPIYPESSWIIPSGKTVKTRFQ
                     PPEGYSRVTAPKDSFAEYLQNLKLKKHGSQVYLFNKKLKYPDDVYDAVVDMDVGKKDL
                     QQCADAVMRLRAEYLYQNKMYEDIHFNFTNGFNASYSKYAEGYRFQITQNSVSWKKKS
                     SPDYSYELFRKYLEVVFNYAGSASLSKELNVVKNISNIEIGDIFIQGGFPGHAVIVVD
                     MAVSKTSGEKVFLIAQSYMPAQDIQILKNPNDEKLSPWYSSKIVDKLITPEWTFEKKN
                     LKRF"
     gene            complement(3824..4609)
                     /locus_tag="PLF66_17085"
     CDS             complement(3824..4609)
                     /locus_tag="PLF66_17085"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HMZ65808.1"
                     /translation="MKPQREIYLFRHGYSRANALRHKKLSFQPFLLLIRKVFGFSFTL
                     FLIQLLELFKTGTILKRLADKDIPLEQIGILQSELTGKFLANRKIHPDLILCSDFLRT
                     RETTQGILKGIQSITEIDHKDKIIYSSLIVERDGGIEFGYPLSYYPVLYPKTNEIYKT
                     THRLDFKPPMGESIKDVRFKRIPNLIYLLNQFNSKTIFIVGHGITNSAILSLLTDTEL
                     EKVKIGMENLGVYHLIEDISGKWLLDPNFSSGQRIDPSIPVTE"
     gene            complement(4625..5938)
                     /locus_tag="PLF66_17090"
     CDS             complement(4625..5938)
                     /locus_tag="PLF66_17090"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HMZ65809.1"
                     /translation="MNTIGNKTKDSEIYKIFQREIETEIVKGEIVRAKFLAGIFFLFG
                     LNFIFFPIFGPKVFIEAFGENFNPLYPPIYFFSIATYFFLNSRLFKIWFNKNRPIPTI
                     PRYINAFIETSIPSISLLYIGHLREPAVYVMLTPPSFVYFIFIIASALQLNFRICVFT
                     GFVAAFEYITITLYLFDRSQGQTLIPFISTIQSHMGKTVFIFLAGVLVGYVTSQIERR
                     LLHSFALLEERNRVTNLFGQHVSPAVVEKLLTQSEFTSEIRHVCMMFFDIRNFTSFSE
                     SKHPEEVVNYLNLIFDFSIDIINRHGGIINKFLGDGFMAVFGAPISSGDDIQNAVKAS
                     LEIIDKVKTEIEKHNIPQTKIGIGLHAGDAVTGNVGSSKRKEYTIIGDVVNLASRIEQ
                     LNKSYSSQLLVSEEVWSKLTDYSAESLGEIVVKGREKPVRIYRLA"
     gene            complement(5984..6538)
                     /locus_tag="PLF66_17095"
     CDS             complement(5984..6538)
                     /locus_tag="PLF66_17095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65810.1"
                     /translation="MTQFLIHSVKTIFKLVFIISYCGLQGLIADEPPSWSGFTRISAN
                     KLFTAKVSQKPKAKNYQSDWILTVSRKGKVLWSCPFEYRFGYPGGILTDDGNYFITVE
                     VWYYKDLPLVDIYKDGRKINTNHLTGESFSSDSKFLHSTTSHFLWFNQSDYYRINLKE
                     NTITLITLDKKEHKIKLNTGEIQK"
     gene            6769..7548
                     /locus_tag="PLF66_17100"
     CDS             6769..7548
                     /locus_tag="PLF66_17100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010570707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HMZ65811.1"
                     /translation="MDFITTEIRNQIVLIGLNRVDASNAFNIQMLHELSKAITDYEAN
                     EELRCAILFAHGKHFTVGLQLEEVSEWILKEGKIHYPEGQLDPFGLNSRKRKKPLVTA
                     IQGFCFTLGIELALASDIRLAAKSARFTQAEVQRGIAPFGGATFRMVEQFGWGNAMRY
                     LLTGDVFGAEEAYRLGLVQEIVEAKNLLDRALQIAETIASNAPLGIKATLESGFKYLE
                     ELEKKVGQDIQNRVIELMRTEDGKEGVQSFLEKRKAKYRGK"
     gene            complement(7645..8010)
                     /locus_tag="PLF66_17105"
     CDS             complement(7645..8010)
                     /locus_tag="PLF66_17105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004787488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HMZ65812.1"
                     /translation="MKGLLLSASISGFLAVAIGAFGAHALKNIFDDKITTFETGNRYH
                     FYHTFASFLSAFLFLFFKEKKFLWSGWLFQIGILFFSFSLYTLSITKISLFGAITPIG
                     GILFLAGWTTLCYTILKIK"
     gene            complement(8007..9182)
                     /locus_tag="PLF66_17110"
     CDS             complement(8007..9182)
                     /locus_tag="PLF66_17110"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010575700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HMZ65813.1"
                     /translation="MSGPLNGVKVIDLSLLLPGPLCSMYLGDMGAEVIKIENPRAYDG
                     TRAMVKSESGMPGLFFMTNRNKKAITLNLKKSESKEILFKLLEKADILLEGFRPDALD
                     EMGIGYDVLKEKFPRLIYCGISGYGTSGPYKDWAGHDGNYLALAGVLDQLGEKDKPSL
                     AGFQLADIGGGTLVALASILAALYSREKTGKGQKIDVSMMESSLQFISLYAGIYLSNG
                     KLPERGNELLSGKLSNYHIYKTKENRFVFLGALEERFFRTFLRQIGKEHLLEEGKNLE
                     EISSEMKETLTEYFEEKTFSDLDFLFQNPDCCLTPIKNLDEVLKDPHLMERSSIFKKN
                     HPTFGEYFQFAAPFRFSNTPCEYTLHPPEHGEHNFEIFTGLGYSKEEIEKFKKGRII"
     gene            complement(9179..11341)
                     /locus_tag="PLF66_17115"
     CDS             complement(9179..11341)
                     /locus_tag="PLF66_17115"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HMZ65814.1"
                     /translation="MQTLFFFFLVSLLIQNSITADDSILNLLYHHPKIKILHKKKTIP
                     KKTSPNTYISKTNSYDSRFLNSQEKIKGVDIVKKTIQITGGSSLEFKISLPLNHHLIF
                     NLHNQKTKEVKDGIKFFLNNNPVYTELKSTAPVNHLRIESNEDILLENIHLLPIEANH
                     NKPDILFIVIDSLRADVCGFNGANFNATPFLDKLAKSSSVFKQHLVNASWTRPSTLAF
                     LTSLYPSKSFINLWDYTVFPEEKASFYRAKIPTLPSTLSTNGYKSIMIGNNPFLTDHR
                     AIGVDVGFEEVIDYSYYENDTIPITENIIAHIKSLPPKEKRRPEFIFLNYNDPHKPYN
                     PPTKYKSQIKNADQFDPRKKDYLGEVAFVDSELEKVFATLKDKNFLDNTLVLITSDHG
                     EVMNPAHGKSKFTGVYTLFGHGQGLYEEDIHTPLLIKFPHQKDHKTVTTVSRSIDLMP
                     TILQEIGYSLPSNIDGLSLQPILEGKEKEERLYYGESRGVIGIRKDGWKWMQKVFFFH
                     RPGAHWDGQVNQEPMYLYNYKKDPEEITPIADESKKNEFKNLANLFLKKTSIYGIRIS
                     NPKGSDSKLIDLSIETNVGKVLLTDDKGNSSHSENFQITQKGIRLSKNFTESESLEFY
                     CIPYPDISFPKFTIAINGNQITKGELGVGEKDIFPGKCHNRAECSEMYLIKHKKPDIP
                     NKFRVQIWLETKSIQVTNEKIKLENDAIDILKKQGYIK"
     gene            complement(11393..11854)
                     /locus_tag="PLF66_17120"
     CDS             complement(11393..11854)
                     /locus_tag="PLF66_17120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004466991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HMZ65815.1"
                     /translation="MSSKKTFSVEKAEDSSGFLLWQVTSIWQREIRKALDPLGLTHSQ
                     FVLLASIFWLILHNEEVTQIKLSDHTKIDPMTTSTVLRTLQAKGYLKRKEHTTDTRAK
                     TVILTKEGEEITKQAVKIVEKFDKQFFASLGDKTNDFNKKLILLLESSEKE"
     gene            complement(11887..12306)
                     /locus_tag="PLF66_17125"
     CDS             complement(11887..12306)
                     /locus_tag="PLF66_17125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000624141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HMZ65816.1"
                     /translation="MITNTYSHTTKEITQEQIWKLISNVNNWKNWDKTIENAELHGEF
                     KNGTFFTLRPKGGPNVKIQLVDVKPNSYFKDLTRFPLAKMYGEHFYENTPEGLKMTIT
                     MTISGFLAFLWNKIVMQDIVNHLAEDIKTQIHEAKKL"
     gene            12479..12991
                     /locus_tag="PLF66_17130"
     CDS             12479..12991
                     /locus_tag="PLF66_17130"
                     /inference="COORDINATES: protein motif:HMM:NF019235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1564 family protein"
                     /protein_id="HMZ65817.1"
                     /translation="MTKIISSSRFSFPRRNMELADFSSTILIPEKYFSIFNEKIILHK
                     GVRSYIHYLLNKYQIYITNGLIPGYSNITTKYQEKGQNLHKISFRPKPENWAELKLYR
                     ISFGMSISAFIVYLLIADSVDLAETFSDYLEGVGILSIPKFDLIAKVYLFDNATYYTT
                     IFQFHRGKYY"
     gene            complement(13036..13500)
                     /locus_tag="PLF66_17135"
     CDS             complement(13036..13500)
                     /locus_tag="PLF66_17135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65818.1"
                     /translation="MISEIESEILITAMKFVQMDDEFVLDTSANISSKPNKILSWFDV
                     YSKNSMISEQIHQDWVQKNLEPISLTPLLNRINVKFLPYDKFSINMNMKDQAWSVFFT
                     ITRPGISSDRRNALLKVTANCLSGPPNYASLLYLERSVHFWNVKSTHGLYNQ"
     gene            13783..15261
                     /locus_tag="PLF66_17140"
     CDS             13783..15261
                     /locus_tag="PLF66_17140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65819.1"
                     /translation="MLRRLHLYLKIFFLIPALYTCNINKKVVGEDVVGENAKPENFKK
                     IAPLTANALGERKNIHEKGWFVIPSSKKSVKTVIENGSMSFRTAKSMLFISIKERAEK
                     YPGNVGETMKMIRSLGKDEEKALQEFSKEVYAGTFNLAKWELEMSGDLFKQSGVDFVT
                     GYITLGDLEKKDREEIFGYIKAYNKDRVKDYSSISELFGEIATKNSNNIAKAWSGAYS
                     RAVLKYSKEYEESGTKQNTLVALWNIFQGYTVALKELALAPVGTTAVESGQMIAVNGV
                     AVPVAYVATFLRQTVVTTGMVVYYPTKLGYRVVSPTLEAGFLSSLGILSASATAPTIV
                     GGTAVGAFNQVATFTGVESTRATASVAGAAYETGATATGMIYDFGKGSTESVYYGLKS
                     GVVLSYTALTVIPTHLLLTVPDATIFLAWDGPRLVIASVRGNYKGFDSLPVGSVVDLE
                     EARKAGKVEVLTDDQTIVKKVLEKEIDLRQDELNKQKEESKK"
     gene            15258..17030
                     /locus_tag="PLF66_17145"
     CDS             15258..17030
                     /locus_tag="PLF66_17145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65820.1"
                     /translation="MKQIQRFLIAIILLGCATTNDPDDPIKNSRKLIKEGHKSLYTNG
                     AFEVKGTSIKLIPSFAEGEIFILGHRVGLAEQAFSENMKKAAESVYILKEGTEISLKT
                     AGKVSEKGEAVNKYIYDSGTVGGIYIIAVSLAASYGIVGNSFDKGMKTHEEVVKSSKE
                     LRAKMDKWADELVESDFPNSSSPEFSERAKEYKRDFRDGVNSFVIGYTNLDDYLAKTV
                     SDADVSTSTFKKGFDKINRFLTDSNEGLTKEFKEVFIEWGSDTAKEFSASGKDYADVV
                     DGAGLSFAILKSFARVTKGVFYDAIIKPLAKIGVLSVGYVAVNAVIYPVMFVSVSGYS
                     VGHVMVEVISVGTKGVIYLVAPSAKLALAGLIGSGRFLAQESYTAADASTRATANASL
                     YTTSKAIKGTGIVTEGAGKYVLANLSLIGVTSAQTVLGGGVAVAGTTAGTTVSTASGA
                     AQVVSYGTSKVAAGTVAVTGTTASLGVAVGNGVYRIGKAIGIPMGITLQTGVVMNYEM
                     IAQLSAHTILAVSDFSYLVLSLEGEKWVVYAVKDKTGKAKALLTGAVVDMEQIRKEGN
                     DVVKVPVSEEEMEKILEKDKQNKN"
     gene            complement(17027..18358)
                     /locus_tag="PLF66_17150"
     CDS             complement(17027..18358)
                     /locus_tag="PLF66_17150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002977225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HMZ65821.1"
                     /translation="MFKSKLNQHIIKLSLPVFFGMLSHTLIQISDTLMVGKLGSEAIA
                     ATGIGANAYFTVLSFLMNGSVGIQILTARRYGEKNDTEVGKIGISIIYFSFIAGLIIS
                     LLGFITANKTIEFISKDPDVIKDASSYLAYRYLGTVFFFPAFAVRGFLDGLGYTYLGM
                     LSAFSATFANIFLNWVLIYGNLGFPALGVDGAAIASALSGLPALFILLVFLFKKEILY
                     FLKKSHYQFDLSVLKNSIEIGLPSAIDGSLTNIAFVIFNKFAGLIGIHSVAASQIILS
                     IISLSFMPGFAFGVAATTILGQAVGAGKYLLAQAGTFRSAKFSAFLMGIMGFLFIVLG
                     KNIIGLFTQEELVIQETYPALVVISLIQIGDAYHMVFASALRSAGLVVWVLIVYVLIS
                     YIVMLPFAYMLGIYFQFGTVGLWSGISIWLLCLSSIFVWKFNKGDWKKVKI"
     gene            complement(18449..19531)
                     /locus_tag="PLF66_17155"
     CDS             complement(18449..19531)
                     /locus_tag="PLF66_17155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010574103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5777 family beta-barrel protein"
                     /protein_id="HMZ65822.1"
                     /translation="MKSIQTIFYISLCLFFTQEILAQAQTKSTFMGSSLINMPTTEDV
                     GKDNLDFRFNHRFGNASQTLRTFGGLDQGANTQLSLDYGLSDKWSIGIARTSAYQTYE
                     ARTKYKILNQNDSMPISLSFFGVAGQETAKETIKYDTYINPPSVANYINLPSQVSSQI
                     DTYIKQEANEYELSDADKRSYLASILISKRFTDRFSLQISPMYVHRNFVKSQLGNDRT
                     GLDIGGRIKLTKRIDLTFEAIFTPKRDYQGNNYYTEDRYTKYPGLNTLTGFEINQQYN
                     NPQGLAYAALRNIYLDKSVKYYYIPISLGVDIETGGHVFQFFVTNSRTIAHTQLLRGA
                     DYDYKKGEWALGFNIHRYFSFAADVE"
     gene            complement(19645..20190)
                     /locus_tag="PLF66_17160"
     CDS             complement(19645..20190)
                     /locus_tag="PLF66_17160"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HMZ65823.1"
                     /translation="MMRLILFICLFYSYVNAVEIKESQLTVTSGKITFLSEAPQETIK
                     GIGKQISGEILFKEKKVKIIIDLTDWKTNNKLQTSHLHENYLETEKFPKAEFDGIIQS
                     YDSKTGDVSVIGSIQLHGVTKKDVSIKGKVTSGEGGYSLVSSFSINLKDYSIEVPKLL
                     ILKVSETVNLEVKLELKPIKN"
     gene            complement(20187..20624)
                     /locus_tag="PLF66_17165"
     CDS             complement(20187..20624)
                     /locus_tag="PLF66_17165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65824.1"
                     /translation="MKKEKLYTVSALFLLITLFNCENKSSDEEEKKRLITGILINTAT
                     GSTSSASSSSSTACSVSTPAFSSLASAGTTTNCARSGCHVGSSPQSGLDMTNYSSVLS
                     RVSSKNPSGSILYQKINGGSMQQYTTSQITTAIFCWIQGGALP"
     gene            20717..22021
                     /locus_tag="PLF66_17170"
     CDS             20717..22021
                     /locus_tag="PLF66_17170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004471555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HMZ65825.1"
                     /translation="MQFVDLHNHLYGSISAEKLFEIGKRNPNPRWQIFTELYSKIYNK
                     NISTETFFEDYSDLTQFKELFLFNKRAPFIEFQSKFNLIIALSRFDEEEIFDVSKSLV
                     IENQEQGVCFVEFRIMYPPNADQSVYQSKTIAACRGLQEGEKITQNKTQGRLVVSLHR
                     DGNFLTQYEWLKDLMRQNELVQKYLVGLDFCYIEEGFPPIHKKTFFEKVLEDNQKNPK
                     MALSILYHVGESFSDKTPKSAVRWIGESALYGAHRLGHAIALGINPEIFKDRVIEESV
                     SERMHQLEFELKHYEEIRTFGRISDRNEILKELDRLKSKDTNIKLSFAMNKNSIDELY
                     TFQEFMIYLIREKTKSVIECCPTSNLYIGMLPDLIDHPIRRFVARNMKVTIGTDDSGI
                     FDTTLPREYNLVANTSLPLKQLEDVRDRSAFYKSEILSGRSI"
     gene            22018..22506
                     /locus_tag="PLF66_17175"
     CDS             22018..22506
                     /locus_tag="PLF66_17175"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HMZ65826.1"
                     /translation="MKEELTHAYERATHILSYASSNLRREDLLAAPGPGLWSITEVIL
                     HIVDCDLVFSDRIKRVIAEENPNLMAFDENKWKENLSYKPENISAAVRLFAANREYIS
                     QILQTIKEEDLERTGMHSQAGAMTLKTIIEKANSHFDHHIKFLYAKRANIGKSVHEIY
                     TK"
     gene            complement(23476..>23814)
                     /locus_tag="PLF66_17180"
     CDS             complement(23476..>23814)
                     /locus_tag="PLF66_17180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65827.1"
                     /translation="EQFKEIQNSSLKSKESVFQITENLGRQFKLQGSVKELLSDLKEQ
                     AIAIRESTKEQKLTIEESNLGLEKISTGSIQLADSAKDLKQLSFELKEDAALLLKQIE
                     FFRIENKVIE"
BASE COUNT         7795 a         3680 c         4384 g         7955 t
ORIGIN      
        1 tggaccctta gcttgatcaa ctaacgttgc agcacgtcca tcccaaaatt gtgcaagatg
       61 aaaaccagcg tttaaaacag ttggagaatt tcttccgccg tttttaccaa acgcacctgt
      121 tgaagtaggt ttattatcaa ctccgccttt tttatctttt acaacatgac agctattgca
      181 tgattgtgta tcatttgcag aaagtcttgt atcaaagtat agtttttcac caaggcttac
      241 tagctctgga gtatcttttt cgctacctgg cattttatct ggaagaattc caaatgctga
      301 ttttgctttt ttttgtaatt cgattgattc ttttgatgga ccgcagttta cgagagccaa
      361 gatcaatact gaaaatatta aatatttctt catactttta atctcctttt ttattagact
      421 tatataaata ataaaaattt gtaaataaga ataattctta tttagagtta cattttttac
      481 aaatgccttt tataatcaaa tgtgtggatt caatttttcc caattgaatt gcattttgag
      541 gtaggttgat ctgttccgta tttgcttttg cgtaagtgaa atcgtagatt gtgtgacatt
      601 tactgcaaat aaaatggtgg tggggttcaa tgtttacttc ataatggttt ttgcctccaa
      661 tacctacaat cctattcact aaattggctt gttctagcga ttcaacattt cgatacacag
      721 tatctagtga gatatttgga aatttagatt ttactttttc aaaaattaaa tcaacggaag
      781 gatgtgagga ggattccaaa aggactttat aaatttcttc tctttgaggg gttattttaa
      841 aaccgctttc cctaaataaa ttgtgaaatt catctagcct aagtttgatt tcatcctttg
      901 gaactttgta attcatattt tttagacttt caaaaatgac tttgcttctt attttatttt
      961 tcagactttt taataaataa ttttttttgt agcagattaa tccaagtcaa ttattctttt
     1021 ccattaatga aatatattta cttaatccta attttatttg gtaatcttta tcccatctac
     1081 tcaaacacgg agttagagaa agatttagaa tttattgaaa ttaaaacaaa gttagatcca
     1141 gcttggaaag tttacactaa aaagaatttt ttatatctag aaagaagaga gccagttcta
     1201 gttatgccag agggtaagac caatatacct cagttaaaat ctgaagcgga cgaagacaaa
     1261 atagctcgta tgaaaaagta tggaatgaag atgaagccga atctcgtcta tcgttttgaa
     1321 aaaagatgga ctgttaatga tacgattcaa tcagatataa taaaaaccga aatcgaaaac
     1381 aaaataaaaa gccttcctga aaaatacaaa attacacacc taatagatga agatttgagt
     1441 aagagaggaa aaatttttta ttctccaaaa acgaagagag ataaacaaag agtcaaagaa
     1501 tattttcaag aaaaagaaaa attagaaaag aaaattcctc tagttcctga ttaccattta
     1561 tcaaagttta gtcttttttt agtttctaaa aaaggagtca gcaatgatta tacagaagtt
     1621 taccctgaat cagtaacagg agaatttatt caagtcgaga agctactttt taaatacagg
     1681 aataaagaat agatttatgc ttgactgatg gatatctgat tcctcctata aattatgaat
     1741 cgatttcttt tttttgtaat tttttgtttt tctctttttt gtaatcaatg ccaaacaaca
     1801 aaagaaaatc tgccaaagga tagagttcct atttttttcc aagtctcaaa cgaacacaaa
     1861 gaattctttg ttgatttagt taaaaacgat tattcggaag cagagataac gactattttt
     1921 caaaatagaa aagatcttgc tcgcactgtt tgtgagatga tctacggtca aaagaaatat
     1981 tcaaaagacg tggtgactca gttgatttca gcagagagtt ttacgaagga gccatttaaa
     2041 acatggggac atcctgttcc aaaattaatt gcaggctgtt ggaatttttt taaactaaat
     2101 gatgttcatc cgaatcgaga ttttgcgtta ctcgcaaagc tctatcctac agaaccaaag
     2161 gattgtttta cagttggttt tatgcaagcc taccttatgc acaatactct tgggtgtaaa
     2221 aatttgacta tggtagattt ggattggaaa attctagaag gtcacaaaca aatgttagat
     2281 ttgtatcatt ctaaatcatt tgattcagaa accaaaatcg caaattctct ctctcaatta
     2341 aaacttggtt ggattgctcg atttgatagt cgacccatgg aagcagaaac caaaactgat
     2401 ctaaatagtc tatgttttgc tgaacatcat aaaatgtgca caaatatact tttagatttt
     2461 cagaaaaact ttctaaatct gaatgcagta tatctaagat tgtctgcttt acatgataca
     2521 aactttaaaa tgaatacgag taatacaaaa ataatttatc ttagcaatgc tatcgacgac
     2581 atatatacaa atcgaaaaca atttgatttc atgataaagg aaacggctaa cgctctaact
     2641 caaaatcaga aagctatttt tgttcaccac gcagcaggtc gttctctatt tggaatctat
     2701 gaattaaaaa agacagaatc gggtggggaa ataacgacaa tctgcaagga taaatattta
     2761 actactccag tagaggctat tacctctgaa tacacaactc acttcgaaaa agtaactata
     2821 aataaaaaat tccctacttg cgaatcttta ttaaaggaat cttcgaattg aagcaaaaaa
     2881 gtagttttat atctataatt gtttttctta gttttttact taatctttac tgtgttccta
     2941 tttaccctga atctagttgg ataatacctt caggtaagac tgttaaaaca agatttcagc
     3001 ctcctgaggg ttattcaaga gtaaccgctc ctaaagattc ttttgccgag tatttacaaa
     3061 acttaaaatt aaaaaagcat ggaagccaag tttatttatt taataagaaa ctcaagtatc
     3121 cggatgatgt gtatgatgcg gtagttgata tggatgtagg aaaaaaagat cttcagcaat
     3181 gtgcggatgc tgttatgcgt ctaagggcag agtatttgta tcagaataaa atgtatgaag
     3241 atatacattt taattttaca aatgggttta atgcaagtta tagtaaatat gcagaaggat
     3301 atagatttca aataacacaa aattcagtta gttggaaaaa aaaatcaagt cctgattatt
     3361 cttatgaatt gtttagaaag tatttagaag ttgtttttaa ttatgctggc tctgcttctt
     3421 taagtaaaga attgaatgtt gtaaaaaata tttcaaatat tgagatcgga gatattttta
     3481 ttcagggcgg atttccgggt catgccgtta ttgtggtgga catggcagtg tccaaaacat
     3541 ctggtgaaaa ggttttttta attgcacaaa gttatatgcc agcacaagac attcaaattc
     3601 taaagaatcc aaatgatgaa aaactaagcc cttggtattc cagtaaaata gtagataaac
     3661 taatcacgcc tgaatggact tttgaaaaaa agaatctgaa gaggttttga aaattattag
     3721 gaggatttga ataggattta ttaaatgcaa gcaaggaaaa ttagattgat tttgggaatg
     3781 ataaaattaa ctacaatttc tatactatac tcgaaaaatc tacttactca gtaactggaa
     3841 tacttgggtc gatcctctga cctgatgaaa aatttggatc taataaccat ttaccgctga
     3901 tatcctctat taagtgatag actcctaagt tttccatacc gattttcact ttttccagtt
     3961 ctgtatccgt tagtagagat aggattgctg agtttgtgat tccatgtcca acaatgaata
     4021 tagtttttga attaaattga tttaatagat aaattaaatt tggaatacgt ttgaatctaa
     4081 cgtcctttat actttctccc attggaggtt tgaaatctag acgatgtgtt gttttataaa
     4141 tctcatttgt tttgggatat aagacagggt aataggagag tggataacca aattctattc
     4201 ctccatctct ctcaacgatt aggcttgaat agataatctt atctttatga tctatttcag
     4261 taatagattg tatccctttt agaattcctt gtgttgtttc tcgggttctt agaaaatccg
     4321 agcaaagaat tagatcgggg tgaatttttc tatttgctaa aaattttccg gtaagttccg
     4381 attgcaaaat tcctatctgt tctaagggta tatctttatc tgctaaacgt tttaagatag
     4441 ttcctgtttt aaatagttcc aatagttgaa tcagaaaaag ggtgaacgaa aaaccaaaaa
     4501 cctttctaat gagtaaaaga aatggttgga aactaagctt cttgtgtctt aatgcatttg
     4561 ctcttgaata gccatgtctg aatagataga tttctctttg aggtttcatc ttaatctccg
     4621 attattaggc taatctataa attctgactg gtttttctct tcccttcact actatttcac
     4681 ctaacgattc ggcagaataa tctgttagtt tactccatac ttcttcagat accaaaagct
     4741 gagaggagta acttttattt aattgttcaa ttcttgaggc tagatttaca acatcaccga
     4801 taatagtata ctcttttctt ttagatgatc caacgttacc cgttacagca tcaccagcgt
     4861 gtaatcctat tcctattttt gtttgtggaa tattatgctt ctctatttct gttttaactt
     4921 tatctatgat ttctagtgaa gccttcactg cattttgaat gtcatcacca cttgaaatag
     4981 gtgctccaaa gactgccatg aaaccatccc ctaagaattt attaataatt ccgccatgac
     5041 gattgatgat atcaatagag aaatcaaaga ttagattcag ataatttact acttcttcag
     5101 gatgtttcga ttctgaaaaa cttgtgaagt tgcgaatatc aaaaaacatc atacagacat
     5161 gtctgatttc gcttgtaaat tctgattgtg taagtaattt ttcaacgaca gcaggagaaa
     5221 catgttgtcc aaacaaattt gtgactctat ttctttcctc taataatgca aaagaatgca
     5281 aaagtcttct ttctatctgg gaagtaacgt atccaactaa gacaccagca agaaatataa
     5341 aaactgtttt acccatgtga gattggattg tgctgataaa agggataagt gtttggcctt
     5401 gtgatcgatc aaaaaggtat aaggtaatag ttatatattc aaatgcagca acaaatcctg
     5461 taaaaacaca aattctaaaa tttaattgta aggctgaagc gattatgaaa ataaaatata
     5521 caaaggatgg aggagttaac atcacatata ccgctggttc acgaagatga ccgatgtata
     5581 aaagtgaaat agatggtata ctcgtctcaa taaaagcatt tatatatcga ggaattgttg
     5641 gtattggtct atttttattg aaccaaattt taaaaagtcg actattgaga aaaaagtatg
     5701 tagcgatcga aaagaaatat atcggcggat agagcggatt aaaattttct ccaaatgctt
     5761 ctatgaatac tttaggtccg aaaatgggaa aaaaaataaa attcaatcca aaaagaaaaa
     5821 agataccagc taaaaattta gctcgaacaa tttccccttt gactatttct gtttcaattt
     5881 ctctttgaaa gattttatag atttcactgt cttttgtttt attaccgatt gtattcatag
     5941 tcgagtgata tcatgatttt atggtgttta atatttctct actttatttt tgaatttcac
     6001 ctgtgtttaa ttttatttta tgttcttttt tatctaaagt gattagagta atcgtatttt
     6061 cctttagatt aattctgtaa taatcacttt gattgaacca aagaaaatgt gatgtagtgg
     6121 aatgtagaaa ttttgagtca ctggaaaagg attcaccagt taaatggttt gtatttattt
     6181 tcctgccatc tttgtaaata tcaacaagcg gcaaatcttt gtaataccat acttcaactg
     6241 tgataaaata atttccatca tcagttaaaa ttcctcctgg ataaccaaat ctatattcaa
     6301 aaggacatga ccatagaact tttcctttcc tagaaactgt aagaatccaa tcagactgat
     6361 aatttttggc tttcggtttt tgggaaactt ttgcagtaaa aagtttattt gcagagattc
     6421 tagtgaaacc gctccaggat ggaggttcat ctgcgattaa tccttgtaaa ccgcagtatg
     6481 aaattatgaa tacaagttta aaaatggttt ttactgaatg aattagaaat tgagtcataa
     6541 taatgcctaa aaaaagtttt tttgaatttt tattttgaaa agagagtaga ctctgtcaat
     6601 tgatagagat taaaaaataa ttaaaaaaaa ctgcaaagag caatcaacaa tccttggatc
     6661 tagagttttt agagttggct tttatagatc taaaaaggat gttatcttaa aataaatagg
     6721 agttttttaa atctagtaga gcctagttaa aaaacattag aggtcattat ggattttata
     6781 acaacagaaa ttagaaatca aattgtgtta attggactca atagagtgga tgctagcaat
     6841 gcatttaata ttcaaatgct tcatgagttg agtaaggcaa taacagatta tgaagcgaat
     6901 gaagaactta gatgtgctat tttatttgca cacggaaaac attttactgt agggttacaa
     6961 ttagaagaag tgagtgaatg gattttaaaa gaaggcaaga ttcattatcc agaaggccag
     7021 ttagatccgt ttggattaaa ttcaagaaag agaaagaaac cgttagttac tgcaatacag
     7081 ggattttgtt ttacattggg aatcgaatta gctctagcga gtgatatacg acttgcagca
     7141 aaaagtgcaa ggtttacaca agcagaagtg cagcgtggaa ttgctccatt tgggggagct
     7201 acatttagaa tggttgaaca attcggctgg ggaaatgcaa tgcgttattt gctaactgga
     7261 gatgtatttg gagcagaaga agcatatagg ttaggacttg tgcaagaaat tgtagaagca
     7321 aaaaatttac tggatagggc tttgcaaatt gcagagacaa ttgcatctaa tgctccactc
     7381 ggaataaagg ctaccttaga aagtggattt aaatatcttg aagaattgga aaagaaagta
     7441 ggacaagaca ttcagaatag agtcattgaa ttaatgcgaa ccgaggatgg aaaggaaggg
     7501 gttcagtcct tcttagaaaa aaggaaagca aaatatagag gaaaataatc cgaaaaaaat
     7561 gaggagtgag gataggatga attcgatagg gctaagttgc tgatcttagc cgaactcagc
     7621 cactaatcct gtgtcgattc tagattattt tatttttaga atcgtataac aaagagttgt
     7681 ccatccagcc aaaaagagaa ttccaccaat aggtgtaatt gctccaaaga ggctaatctt
     7741 ggttatactc aatgtatata aactgaatga aaaaaataaa atcccaattt gaaaaagcca
     7801 accagaccat aaaaactttt tctctttaaa aaataaaaac aaaaaagcag aaagaaaact
     7861 agcaaaggta tgatagaaat gatatctatt ccctgtttca aatgtagtaa ttttatcatc
     7921 aaaaatattt ttgagagcat gggctccaaa tgcacctatc gcaacagcca gaaatccgga
     7981 aatggaggct gagagtaata atcctttcat ataatacgac cttttttgaa tttttctatt
     8041 tcctctttgg aatatcctaa tcctgtgaat atttcaaaat tgtgttcccc atgttctggt
     8101 ggatgtaaag tgtattcgca tggagtattc gaaaatcgaa aaggtgctgc aaactgaaaa
     8161 tactctccaa aagttggatg attttttttg aagatagagc ttctctccat taggtgcggg
     8221 tcttttaaaa cttcgtctag gtttttaatt ggagtgaggc agcaatctgg attttgaaat
     8281 agaaaatcga gatcactaaa cgttttctct tcaaagtatt cggttaacgt ttctttcatt
     8341 tcacttgaaa tttcttctaa attttttcct tcttctaata agtgttcttt tcctatttgt
     8401 cttaggaagg ttctaaaaaa tctttcttct aaggctccta gaaaaacaaa tcggttttct
     8461 tttgttttat aaatatgata attggaaagt ttaccggata acaattcgtt gcctctctct
     8521 ggcagttttc cattagataa ataaattcct gcatagaggg aaataaactg taagctagat
     8581 tccatcatgg atacatctat cttttgacct ttgcctgttt tttctctgga ataaagtgcg
     8641 gctaagattg aggctagagc aacgagtgtt cctcctccaa tgtctgctaa ttgaaatccg
     8701 gctaacgatg gtttgtcttt ttcacctaat tgatctaaaa cgcctgcaag tgctaggtaa
     8761 tttccgtcat gccctgccca atctttataa ggtccacttg tcccatatcc agaaattcca
     8821 caatagatga gccgtggaaa tttctctttt agaacatcgt aacctattcc catttcgtct
     8881 agcgcatcag gtctaaaacc ttctaatagg atatctgctt tttcgagaag tttgaataat
     8941 atttcttttg attctgattt ctttagattt agagtgattg cctttttatt acgatttgtc
     9001 ataaaaaata aaccaggcat tcctgattct gattttacca tggctcttgt gccgtcatac
     9061 gctctagggt tttcgatttt gatgacttct gcgcccatat cacctaaata catagagcaa
     9121 agaggacctg gtaaaagtaa acttagatca atgactttga caccatttaa tggaccactc
     9181 attttatata cccctgtttt tttaaaatat caattgcatc gttttctaat tttatttttt
     9241 cattggtaac ttgtatcgat tttgtttcga gccaaatttg aactctgaat ttattcggaa
     9301 tatcaggttt tttatgttta atgagataca tctccgaaca ttcggctcgg ttgtggcatt
     9361 tccccgggaa aatatctttt tctccgacgc ctaattctcc tttggtgatt tggtttccgt
     9421 ttatggcaat ggtaaatttt ggaaacgata tatctggata gggaatgcag taaaattcaa
     9481 gactttcact ttcggtaaag tttttagata gacgaattcc tttttgagtt atttgaaaat
     9541 tttctgaatg agaagagttt cctttatcat ctgttaacaa gactttccca acgttagttt
     9601 ctatagatag atcgattagt ttagaatcac ttcctttggg atttgaaatc ctaattccat
     9661 agatggatgt tttctttaaa aagagattcg ctagattttt aaattcattt ttttttgact
     9721 cgtctgctat tggtgtaatc tcttctgggt cttttttgta attatacaaa tacattggtt
     9781 cttgatttac ctgaccgtcc caatgagcgc ctggtctatg aaagaagaat actttttgca
     9841 tccacttcca gccatctttt cggataccga tcactcctct tgattcacca taataaagtc
     9901 tctcctcttt ttctttgcct tctaggattg gttgaagact taagccatct atattacttg
     9961 gaagagaata tccaatttct tgtaatatcg ttggcattag atcgatagaa cgactaacgg
    10021 ttgtaactgt tttatgatct ttctgatgag gaaatttgat taggagtggc gtatgaatgt
    10081 cttcttcata caagccttgt ccatgtccaa agagagtgta aacaccggta aattttgatt
    10141 tgccatgagc aggattcatt acttctccat gatcggaagt aattagaact agcgtattat
    10201 ctagaaagtt tttatctttt agtgtggcaa aaactttttc taactcagaa tctacaaagg
    10261 cgacttcacc taggtagtct ttctttcgag gatcgaattg gtctgcattt ttaatttggg
    10321 atttgtattt tgtgggagga ttataaggtt tatgtggatc attataattc agaaaaataa
    10381 attctggtct tcttttttct tttggaggaa gagatttgat atgagcaatt atattttctg
    10441 taattgggat tgtatcattt tcataatagg aataatcgat tacttcttca aatcctacat
    10501 cgacaccaat tgctctatgg tcggttagga atggattatt tcctatcata attgatttat
    10561 aaccgttagt agaaagagta gaaggaagtg taggaatttt cgctcgatag aatgatgctt
    10621 tttcttccgg aaaaactgta tagtcccaaa gattaataaa acttttggat ggatataaac
    10681 ttgttaaaaa agcaagtgta gaaggacgag tccaactagc gtttactaga tgttgcttaa
    10741 aaacggaact agatttcgcc agtttatcta gaaatggagt tgcattaaag tttgcaccat
    10801 tgaatccgca tacatctgca cgcaaagaat caattacaat aaaaagaata tcaggcttat
    10861 tgtgattagc ctcaattggt aataaatgga tattttctag gaggatatcc tcattagatt
    10921 caattcgtaa atgatttaca ggtgcagtag attttaattc tgtataaacg ggattattgt
    10981 ttagaaaaaa ttttattcca tctttaactt cttttgtttt ttgattatgt aaattgaaaa
    11041 ttagatggtg attgagtggt aatgaaattt taaattcgag actggaacca cctgttattt
    11101 gaatcgtttt ttttacaata tctacacctt taattttttc ttgagagttt agaaatcttg
    11161 agtcataaga atttgttttg gatatataag tgttaggtga tgttttttta gggatagtct
    11221 tttttttatg taaaattttg atcttcggat gatggtaaag taaatttaaa atagaatcat
    11281 ctgcggtaat ggaattttga attagtaaag aaactaaaaa gaagaaaaat aaagtctgca
    11341 tgtttgtagc aaaagtataa aactagaaga atgttgcaag aaagaattgg aattattctt
    11401 tttcagaaga ttctaaaaga aggattagtt ttttattgaa atcatttgtc ttatctccaa
    11461 gggatgcaaa gaattgttta tcgaacttct ctacgatttt cactgcttgt tttgttatct
    11521 cctctccttc ttttgttaga attaccgttt tggctctagt atctgtggta tgttcttttc
    11581 tttttaaata acctttagct tggagtgttc ttagaacagt ggatgttgtc ataggatcta
    11641 ttttggtatg atcggaaagt ttgatctgtg ttacttcctc attatgaagt atgagccaaa
    11701 agatactagc tagtaaaaca aattgtgagt gtgttagacc caaggggtct aacgcttttc
    11761 gaatttctct ttgccatatg ctggtgactt gccataataa aaaaccagaa ctatcttcgg
    11821 ctttttcaac actgaatgtt tttttactgc tcattcttgt tccatattct aatacagaag
    11881 aaaaaattat agcttttttg cttcatgaat ttgtgtttta atatcttccg ctagatgatt
    11941 cacaatatct tgcattacga ttttattcca gagaaaagct aggaatccac tgattgtcat
    12001 tgtaatagtc attttaagac cctctggagt attttcgtaa aaatgctctc catacatttt
    12061 tgcgagaggg aaacgagtta aatctttgaa ataactattt ggtttaacat ctaccaattg
    12121 aatttttaca tttggtccac ctttaggtct tagagtgaaa aaagttccat tcttaaattc
    12181 accatgaagt tctgcatttt caatggtttt atcccagttc ttccagttgt tcacatttga
    12241 gatgagtttc caaatttgtt cctgcgtgat ttcttttgtt gtatgagaat aggtgtttgt
    12301 gatcatattc gcctctttac ttgattgtat aaaatacata ttataagtat acatataata
    12361 tgtaaacata aaaaataatt ctatatttac ataattttat ttaaaaagtc ccgcacataa
    12421 ggaaagattt ttgaccctat attaaatagg ataggtttat aaaaaggagt tcttttctat
    12481 gacgaagata atttcaagta gtaggttttc atttccacgc cgaaatatgg aattggcaga
    12541 ttttagctca acgattctaa ttcctgagaa gtatttctct atttttaatg agaaaatcat
    12601 tctgcataaa ggagttcgta gttatattca ttatctcttg aacaagtatc aaatttatat
    12661 tacaaatggt ttgattcctg gttattctaa tataaccaca aagtatcaag agaaagggca
    12721 aaatcttcat aagatttcct ttcgtcctaa accggaaaat tgggctgaac tgaaacttta
    12781 tcgtattagc tttggtatgt ctatctctgc ttttatcgta tacttactaa tagcagactc
    12841 agtagatctt gcagagactt tttcggatta cttagagggt gtcggaattt tgtcgatccc
    12901 taagtttgac ttaatcgcga aagtctacct atttgacaat gcgacctatt atactacaat
    12961 atttcagttt cataggggaa aatattatta aataaaaaaa gaccctaaca tctaagacag
    13021 aattagagat aaagactatt gattatagag accatgggta gacttaacgt tccagaaatg
    13081 aacagagcgt tctaggtaaa gaagacttgc gtaatttggt ggaccggaaa gacagtttgc
    13141 ggtaactttt agaagagcat ttcgtctatc ggatgaaata cctggacgag tgatagtaaa
    13201 aaaaacagac catgcttggt ctttcatatt catattgatt gagaatttgt catagggtaa
    13261 aaatttaaca tttatacgat tgaggagtgg agtaagagag atcggttcca agtttttttg
    13321 aacccagtct tggtgaattt gttctgatat catagagttt tttgaataaa catcaaacca
    13381 agacagaatt ttatttggtt tgcttgaaat atttgcagaa gtatccaaga cgaattcatc
    13441 atccatttgg acaaatttca ttgctgtaat aagtatttca gattcaattt ctgagatcat
    13501 aaatgtattg aattgtgaaa aggagaggaa ttccagtctt ttttataaga aaattcaaaa
    13561 gtatttatct aataaatctt atcttggaat gtagaggata gaataattgt tttgaatcga
    13621 gcagagaagg atcaatttgg gtgtattttt caaagataag agaatgagtt tatcagcttt
    13681 cgcgttggac aaatcattaa cgattgactt tttcgaaaag aatggatgaa tacaaatgtt
    13741 aatgattcag agatggtctt tgggaagaat aaggagttta gtatgttgcg cagattacat
    13801 ttgtatttaa aaatattttt tttgataccg gcattgtata catgtaatat aaacaagaaa
    13861 gttgtgggtg aggatgttgt tggtgagaat gcaaagccgg agaatttcaa gaagattgcc
    13921 cctttgactg caaacgcatt aggagaaaga aagaatatac atgaaaaggg gtggtttgtt
    13981 attccttcct caaaaaaatc tgttaaaaca gttattgaaa atggttctat gtcttttcgg
    14041 acagctaagt caatgttatt tatttccatc aaagagagag cggaaaagta tccgggtaat
    14101 gttggggaaa caatgaaaat gattcgtagt ttgggtaaag atgaggagaa agctttgcag
    14161 gaatttagca aggaggttta tgctggaaca ttcaatcttg cgaaatggga attggaaatg
    14221 tcgggagatt tgtttaagca atcgggagta gattttgtta caggatatat aacgttaggt
    14281 gatttagaaa agaaggatag agaagaaata tttggatata taaaagcata taataaggat
    14341 agagtaaagg attattctag tatatcagaa ctctttgggg agattgcgac gaaaaatagt
    14401 aataacattg ccaaggcttg gtcgggggct tacagcaggg cagttctaaa atactcaaag
    14461 gaatatgaag agtccggaac aaaacaaaac acattagtag cattatggaa tatttttcag
    14521 ggttacactg tagccttaaa agaattagct cttgcacctg tcggaactac ggctgtggaa
    14581 agtggtcaaa tgattgcagt aaatggggtt gctgttccgg ttgcctatgt agctactttt
    14641 ttaaggcaaa cagtagttac tacaggaatg gtagtctatt atccgacgaa gttagggtat
    14701 agagtcgtta gtcctacatt agaggcagga tttttgagtt ccttgggaat cttgtcagca
    14761 agtgcgacag cgccaacgat tgtaggggga accgctgttg gagcatttaa tcaagttgca
    14821 acttttactg gagttgaatc tactcgggcg actgcatctg ttgcaggtgc ggcttatgaa
    14881 acgggagcga ctgcaactgg gatgatttat gatttcggga aaggctctac tgaaagtgtt
    14941 tactatggtc ttaagtcagg tgttgtgttg agttatacag cgttaactgt aatccctact
    15001 catttattat taacagtccc ggatgcaact attttcttgg catgggacgg accgagatta
    15061 gtcattgcaa gcgttagagg aaattataaa ggatttgata gtcttccagt tggatccgtt
    15121 gtagatttag aagaagctag gaaagctgga aaagtagaag tgttaacaga tgatcaaact
    15181 attgtgaaaa aagttttgga gaaggaaatc gatttaaggc aagatgaatt aaataagcag
    15241 aaagaggaga gtaaaaagtg aaacagattc aaagattttt gattgcaata atactactcg
    15301 gatgtgcaac aacaaatgat ccagatgatc caattaagaa ttcaagaaaa cttattaaag
    15361 agggtcataa gtctctatac actaacggtg cttttgaagt aaaaggaaca tcgattaaat
    15421 taataccatc atttgcagaa ggagaaatat ttattttagg tcacagggtt ggattagccg
    15481 agcaagcatt ttctgaaaat atgaaaaaag cagcagagtc agtatatatt ttaaaagaag
    15541 gaactgaaat atctttaaaa actgctggga aggtttcaga aaaaggagag gcagttaata
    15601 aatatattta cgattcagga acggtaggcg gaatatatat aattgcagtc tcccttgctg
    15661 cttcttatgg aattgttggg aactcttttg ataaaggaat gaagactcat gaagaagttg
    15721 taaaatcatc gaaggagtta cgagcaaaaa tggacaaatg ggcagatgag ttagtcgagt
    15781 ctgattttcc aaattcatcc tctccggagt tttcggagag agcaaaggag tataagaggg
    15841 atttcagaga tggagtaaat tcgtttgtca taggttatac aaatctagat gattatttag
    15901 ccaagacagt ctccgatgca gatgtatcca cgagcacctt taagaaaggc tttgataaaa
    15961 taaatcgatt tttaacggat agtaatgagg gtcttaccaa agagtttaaa gaagtattta
    16021 ttgagtgggg aagtgataca gcaaaagaat tttccgcatc aggaaaggat tatgctgatg
    16081 tagtggacgg agctggctta tcttttgcta ttttgaaatc ctttgcaaga gttacaaaag
    16141 gagttttcta tgatgctatt atcaaacctc tagcaaaaat tggagtcctt tctgtcggat
    16201 atgtagcagt aaatgctgtg atttatccag ttatgtttgt gagtgtgtct ggatatagtg
    16261 ttggtcatgt aatggtcgaa gtaatatctg ttggaacaaa gggtgtaatc tacttagttg
    16321 caccgagcgc aaaattggca ttagccggtt tgattggaag tggtagattt ttagcacaag
    16381 aatcatacac tgctgcggat gcgtctacac gtgcaactgc aaatgcgagt ctgtatacaa
    16441 catctaaggc aataaaggga acaggaattg ttacagaggg tgcaggtaaa tatgtgttag
    16501 ccaatctaag tttaattgga gtaacttcag ctcaaactgt tttaggtggc ggagtagctg
    16561 tcgcagggac tactgcggga acgacagttt ctacagcatc tggcgcagca caggttgtat
    16621 cttatggcac aagcaaagta gcggctggga ctgttgctgt aacaggaact actgcatctc
    16681 ttggagtagc cgtaggaaat ggcgtatatc gtattggaaa ggcgatcggt ataccgatgg
    16741 gcattacctt acaaactggt gttgtcatga actatgaaat gatagcacaa ctctccgctc
    16801 atacgatatt ggcagttagt gatttttcct atttagtttt atctttggag ggcgagaaat
    16861 gggttgtcta tgctgtaaaa gataaaacag gtaaggcgaa agcgttatta acaggcgctg
    16921 tcgtagatat ggagcaaatt cgtaaagagg gcaatgatgt agtaaaagtt cctgtcagcg
    16981 aggaagaaat ggaaaaaata ctagaaaaag acaaacagaa caagaattaa attttaacct
    17041 ttttccagtc tcctttgttg aacttccaga caaagataga agaaagacaa aggagccaaa
    17101 tggaaattcc tgaccacaaa ccaacggtcc caaattgaaa gtagattcca agcatatagg
    17161 caaaaggtag cataacaata tacgatatta gaacataaac aatcagcacc caaacaacta
    17221 agcctgcact tctgagtgcc gaagcaaaaa ccatatgata tgcatcccca atttgtatca
    17281 aagaaattac tactaaagct ggatatgttt cttgtattac taattcttct tgtgtaaaga
    17341 gtccgataat atttttacct aatacgatga ataagaatcc catgatgccc attaaaaaag
    17401 cagagaattt agcagatcta aaagtgcctg cttgggctaa caggtatttg cctgcgccca
    17461 ctgcctgacc taaaatagtt gtagcagcaa ccccgaatgc aaaccctggc atgaaggata
    17521 aactaataat acttagaatg atttggctcg ctgccacgga atggattcca atcagaccag
    17581 caaatttatt aaaaatgaca aaggcaatat ttgtaagaga accatcaatg gcagaaggaa
    17641 gacctatttc aatggaattt tttaagacgg aaagatcgaa ctgataatga gactttttta
    17701 ggaaatagag tatttctttt ttgaagagga ataccagtaa tatgaataat gcaggcagtc
    17761 ctgaaagggc agaagcgata gccgctccat ctacgccaag tgcaggaaat cctagatttc
    17821 cataaatcaa aacccaattc agaaaaatat ttgcaaacgt tgcactaaaa gcagagagca
    17881 ttcctagata ggtataacct aatccatcta gaaaacctcg aactgcaaaa gctgggaaaa
    17941 aaaatactgt tcctaaatac ctgtaagcca aataagaaga cgcatcttta attacatctg
    18001 gatctttaga aataaattcg attgtcttat ttgctgtgat aaaacctaaa agagagataa
    18061 ttaaaccagc tataaatgaa aaatatatga tactaattcc aatttttcca acttccgtat
    18121 cattcttttc tccgtatctt cttgcggtta atatttgtat gccaacagac ccattcatta
    18181 gaaatgaaag aactgtaaag tatgcgttag ctcctattcc agttgctgca attgcctcag
    18241 atccaagttt accaaccata agagtatcac tgatttgaat cagtgtgtga ctcaacattc
    18301 caaaaaatac aggtagggat agttttataa tatgttgatt taattttgat ttgaacattt
    18361 taaacttatt tactattttc tataatccac tcagaagtct ttcctatttt ttgaaattgg
    18421 atctatttta aatcaatcaa aagatatttt attcaacatc cgccgcaaat gaaaaatagc
    18481 gatgaatatt aaagccaaga gcccattcgc cttttttgta atcatagtct gcaccacgta
    18541 gcaattgagt gtgtgcaatg gttctagagt ttgttacaaa gaactgaaat acatgtccac
    18601 cggtttctat atcaacaccc aaactgattg ggatgtaata atattttact gatttatcta
    18661 ggtaaatatt tctgagggct gcgtatgcca gaccttgtgg attgttatat tgttgattga
    18721 tttcaaatcc agtcagtgta tttaaacctg gatatttcgt gtaacgatct tccgtataat
    18781 aattatttcc ttgataatct cttttaggtg taaagattgc ttcgaaagta agatcaatcc
    18841 ttttcgttaa tttaattctt cccccaatgt ctaatcctgt tctatcattt cctaactgac
    18901 tctttacaaa atttctatgc acatacatag gagaaatctg caaggaaaat ctatcagtaa
    18961 agcgtttcga tattaagata gatgccaagt aactacgctt atccgcatca ctcaattcat
    19021 attcattcgc ttcttgtttt atataggtat caatctgaga agaaacttgt gacggtaaat
    19081 taatataatt tgctacagaa ggtgggttga tataagtatc gtatttgatt gtctccttgg
    19141 ctgtttcttg tcctgcaact ccgaagaaac tcaggcttat tggcatagaa tcattttgat
    19201 ttaaaatttt atatttagtt ctagcctcat aagtttgata agcagatgtt ctcgcaattc
    19261 ctatgctcca cttatcgctt aacccatagt ctagggataa ttgcgtgtta gccccttgat
    19321 ctaatcctcc aaatgttcgg agtgtttgac ttgcattacc gaatctatga ttaaatctaa
    19381 aatctaaatt atcttttcct acatcttctg ttgtaggcat attgattaga cttgatccca
    19441 taaatgtaga ttttgtctgg gcttgtgcta gaatctcttg ggtgaaaaat agacagagtg
    19501 aaatgtaaaa tatagtttga attgatttca ttatacacct tttatatttt tatttacttg
    19561 cgataaggat tgagtcgaag ttaagaaatt tatgaagctc ttaaccttac gagaatcccg
    19621 ttctaatgga gtccgaatcg cttactagtt tttaattggt tttaattcta gttttacttc
    19681 taggtttaca gtttcgctta cttttaaaat aagaagtttt gggacttcaa tcgaataatc
    19741 ttttaaatta atcgagaaag acgatactaa tgagtatcct ccttcgcctg acgtaacttt
    19801 tccctttatg ctcacatctt ttttagttac accgtggagt tggatggatc caataacaga
    19861 aacatcaccc gttttagaat cgtatgattg aatgattccg tcaaactctg ccttaggaaa
    19921 tttttctgtc tccaaatagt tttcatgtaa atgagaagtt tgcagtttgt tgtttgtttt
    19981 ccagtctgtt agatcaataa tgatttttac ttttttttct ttaaataaaa tctctccgga
    20041 aatttgcttt ccaatgcctt taattgtttc ttgaggagct tcacttaaaa atgtaatctt
    20101 tcctgaagta actgttaatt gtgattcttt gatttcaact gcatttacat aagaataaaa
    20161 taagcagata aagagtatta gccgcatcat ggtaaagcac ctccttgtat ccaacaaaag
    20221 attgctgtag taatctgact tgtcgtatat tgctgcatgc tacctccgtt tattttttga
    20281 taaagaatgc tacccgatgg atttttagag cttactcttg ataagactga gctataattt
    20341 gtcatatcta gaccgctttg tggactagaa ccaacatggc atcctgagcg agcacagttt
    20401 gtagtagtcc cagcggaggc taaagaagag aatgcaggcg tagaaaccga acaagctgta
    20461 gaactagaag aagatgcgga agaggttgac ccggtagctg tattgatgag gattcctgtg
    20521 attagccgct ttttctcttc ctcatcagaa cttttattct cacaattgaa tagtgttatt
    20581 agtagaaata acgcagagac tgtatataat ttttcctttt tcatagttag gcaccgactt
    20641 gtcattattc tattttcatt ctttttgtga ataaattatt atttacaaaa gactttattt
    20701 tacagttcct tttagtatgc aatttgtaga tttacataac catctttatg gatcaatatc
    20761 cgcagaaaaa ctatttgaaa ttggcaaaag aaatccaaat ccacgctggc agatttttac
    20821 ggaactttat tcaaaaatat ataacaaaaa tatttcaact gaaacgttct ttgaggatta
    20881 cagtgatttg actcagttta aagaattatt tctatttaat aaaagagcac cttttatcga
    20941 atttcaatct aagtttaatt taattattgc tctatctaga tttgatgaag aggaaatatt
    21001 tgatgtatcc aagtcactgg tgattgaaaa ccaagaacaa ggagtttgct ttgtagaatt
    21061 tagaattatg tatccaccaa atgcagatca gtctgtttat cagtctaaaa ctatcgctgc
    21121 ttgtagggga ttgcaagaag gggaaaagat tactcaaaat aaaacacagg gtaggcttgt
    21181 tgtttctctt catcgagatg gaaattttct tactcagtat gaatggctta aagatttaat
    21241 gagacaaaat gagcttgttc agaagtattt ggttggattg gatttttgtt atattgaaga
    21301 aggattccct cccattcata aaaaaacatt tttcgagaaa gtattagaag acaatcaaaa
    21361 gaaccctaaa atggcacttt caattttata tcatgtagga gagagttttt cagataaaac
    21421 acctaaatcg gcagttcgtt ggattggaga atctgcactt tatggcgcac atcgtctcgg
    21481 gcatgccata gcacttggta ttaatccaga aatttttaaa gacagagtga ttgaggagtc
    21541 ggtttcagaa agaatgcatc aactcgagtt tgaattaaaa cactatgaag aaattcgaac
    21601 tttcggaaga atctctgatc ggaatgaaat attgaaagaa ttagatcgtt taaaatcaaa
    21661 ggatacaaat ataaaattgt catttgcaat gaataaaaat tctatcgatg agctctatac
    21721 atttcaggaa tttatgattt acttgattcg tgagaaaaca aaatctgtta ttgaatgctg
    21781 tcctacttca aatctatata ttggaatgct tcctgattta atagatcacc caatccgtag
    21841 atttgtagct agaaatatga aagtaacaat cggaactgac gattcgggta tttttgatac
    21901 tacacttcca agagagtata atcttgtggc taatacaagt ttgcctctaa agcagttaga
    21961 agatgttaga gatcgatcag cattttataa atctgaaata ttatcaggaa ggtctatatg
    22021 aaagaagaac ttacacatgc ttatgaaaga gcaactcata tcctaagtta tgcaagctca
    22081 aatttaagga gagaagattt gcttgcggca cctggccctg ggctttggag tataacggaa
    22141 gtaattctac atattgtaga ttgtgacctt gttttcagtg atcgaattaa acgtgttatc
    22201 gctgaggaaa atccaaatct aatggcattt gatgagaata aatggaaaga aaatctttct
    22261 tataaaccag aaaatatttc tgctgctgtt cgtttatttg ccgcaaatcg tgaatacatt
    22321 tctcaaatat tgcaaacgat taaagaagag gatttagaaa gaactgggat gcactctcaa
    22381 gcgggagcaa tgactcttaa aactattatt gaaaaagcaa attctcattt tgatcatcat
    22441 attaaatttt tatatgcaaa gcgggctaac ataggtaaat cggtgcatga gatttataca
    22501 aagtagaatc aaaactcaga ttctatttgt atccaactaa aactgatagc caatccaagc
    22561 ttcattgttc gatacaaaat ttattgcggt gctactgcaa taaatttcat gaattcgaca
    22621 agctcaccca gcgctcagta accggatttt ttcctattcg gaatcatcct ccctttcgct
    22681 agacgctcac caaccgccaa gcgatgaccg gactttcttg tttgagtagt tacgaaaaaa
    22741 tccggtcact gagtgaaatt tttttcgaat gaaaaaaatt ttgtatcgaa tgtgaccttt
    22801 tagaaattcg gattcactcg atgtccgaac tttaccactc aagaaaactt ctagaaaatt
    22861 gtcgtacact aaaaaatttt accccgttta attcaaaaca tctctttatt aaagactcgc
    22921 aatccttttt tactttaagc cattcaattg taagatagaa atgggagaag ttgggtgcaa
    22981 attaggcatt agccgctaga aagaaaatgg aggctgaaac tttaagtttt ctttttaatc
    23041 gggaatctct attcccgtgc atacagcaaa taacgttttc tctcgagata ccaacccggc
    23101 tttcagataa agctgtcaag ttaaggattc tcaccgatga caccgataac ggaacgatta
    23161 aaaatcaatg gatacttatg agttatcgta tcttttatca gtgttaattc cagccttcgc
    23221 tctaagtatt cgcaaactac gccttcgcct gttaatttgt ggtaatcatt aacttgaaag
    23281 cattgcgcat cgcgttgctc ttgaaaatgg attattaaat gacattacgc atgagatggt
    23341 atgtatgaat aaagtaaatg atatcagtat attacgttta tataatggat taatatatag
    23401 gcgggtttgg gcagcagccc aagttgccga catgctccct gtaaacccac attgcatctt
    23461 ttcgcgtaag gtgagttatt caataacctt attctctatg cggaaaaact ctatttgttt
    23521 taataaaaga gcagcatcct cttttaattc gaaagatagt tgctttaaat cttttgcaga
    23581 atcagcgagt tgaatggagc cagtggatat tttttctaat ccaaggtttg attcttcgat
    23641 tgtgagtttt tgttctttgg tagattctcg aatcgcaatg gcttgctctt ttaaatccga
    23701 tagcaattct tttactgaac cttggagttt gaattgtctt cctaaatttt ctgtaatctg
    23761 aaaaacggac tcctttgatt ttaaagatga attttgaatt tctttgaact gttc
//
LOCUS       DAOQOS010000085        23620 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_1021_length_23620_cov_5.987269, whole genome shotgun sequence.
ACCESSION   DAOQOS010000085 DAOQOS010000000
VERSION     DAOQOS010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 23620)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23620)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23620
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1021_length_23620_cov_5.987269"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..372
                     /locus_tag="PLF66_17185"
     CDS             <1..372
                     /locus_tag="PLF66_17185"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HMZ65828.1"
                     /translation="IVDHFKKLNSDFGQEAGDLILNSLGIIAKEHFSKENLLFRLEGK
                     KFGVILPNTTRDKALLLLDSFRDKVASIPINSNGNRVEYTISIGLSVSENQSFDTKLR
                     EAEEELSQARKNGRNQVSAKS"
     gene            373..1002
                     /locus_tag="PLF66_17190"
     CDS             373..1002
                     /locus_tag="PLF66_17190"
                     /inference="COORDINATES: protein motif:HMM:NF017206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HMZ65829.1"
                     /translation="MNRILIIGATGRVGQRLVQYSLNSNYKVTALVRDKSKLKSTNPN
                     LEVIVGSVLDPQIIEEAIQGKEFVLTALSGRSTKPDYSVLSKGIENVVTCMEKAKVKR
                     LISVAGAGILDDSEFGLRRNRPNYPEIFKLVSAENWKIYEYLKTKEINWTLVCAPEMP
                     EEKRTGVYRATNDYLPLGGRRISVEDVADYILKILDEPETFKKRIGISY"
     gene            complement(999..1283)
                     /locus_tag="PLF66_17195"
     CDS             complement(999..1283)
                     /locus_tag="PLF66_17195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65830.1"
                     /translation="MNLPKDILDFIEKKFLDTDTILDHLERLNQYLDEMDVKESRDRI
                     IRSILFLTAANEDLFRQYLEMTKLDYRDILYLAEYENDKQVRNFLNPISD"
     gene            complement(1284..1634)
                     /locus_tag="PLF66_17200"
     CDS             complement(1284..1634)
                     /locus_tag="PLF66_17200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65831.1"
                     /translation="MIFISCRSFPGKRALNLSYTNPSNCGFFQFYRDGSFRYDLGSSE
                     QCVDNKSKPRINDPTLGNIGKYSFESKTDVTPYLIQSDKEIDEFKVRFDEDLKNLFVK
                     RKGSSLEEKFGKLK"
     gene            complement(1658..2194)
                     /locus_tag="PLF66_17205"
     CDS             complement(1658..2194)
                     /locus_tag="PLF66_17205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65832.1"
                     /translation="MKKGFIFVYIFFTLILFQCSSVQVIPEPNLDQVTVKSEPVCTVF
                     TWYWPFCTQGRAKSFLIKGIYNNSTGEKKYFLDYHLDMLDTDLPVGISVYLDGVYYNL
                     KKVSTDYSDILKIESEINLDLISKMNSAKNNFSFSYSNRKETHNFTLSSGDTKDFLKQ
                     LNDVVKRINAQEKLKIVN"
     gene            2331..3551
                     /gene="serA"
                     /locus_tag="PLF66_17210"
     CDS             2331..3551
                     /gene="serA"
                     /locus_tag="PLF66_17210"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002761132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HMZ65833.1"
                     /translation="MISFPKEKINVLLLENIHENAFNLFKNDGFSVKLMKDAYLEDDL
                     CEAIKDVHILGIRSKTNVTEKVFQNANKLLTLGCFCIGTNQVDLSVAEKKGIPVFNAP
                     YSNTRSVAELVIAEIIFLARKTGDQSRDAHNGKWNKIATGCFEVRGKTLGIVGYGHIG
                     SQVSILAESFGMKVIFYDIQTKLPLGNAQPVDTYEGVLKEADFLTFHVPESPDTMNLL
                     GAKEIAKMKKGSYLLNLSRGRVVDIDALAAALKSGHLAGAGIDVFPEEPKSNKDPFVS
                     PLQNIPNVILTPHIGGSTEEAQRNIGLEVANKLLRYINNGSTSFAVNFPNVELPLLKD
                     NHRILNVHKNQPGFLKDINKVVSDLGANITAQFLSTTADIGYLIMDINKQLGDQVKEE
                     IEKNPLSIKTRILY"
     gene            complement(3940..5202)
                     /locus_tag="PLF66_17215"
     CDS             complement(3940..5202)
                     /locus_tag="PLF66_17215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65834.1"
                     /translation="MKITLAGIEFDDLREFTKFVNDLDLLGIVQLRESIRDKKGNILI
                     KDNVHIKESALKKLESLSGQYESKFKVNMTSDVTGRIKKALTAEIMPGIQKSKNEFIS
                     ILYNNDQSSVVSTESLINSSFYTNDLVLVLYKIMLEQYDFFQHIVSMGLICLGTVVQR
                     SYNIKLIHRYAFLTGMFSDVIFFNTDYWRKSNLDDTLMERLAKLNAQIAQNVNMPAQI
                     VSAIQNHIVKGVYVVDKPDPMDFNVLNANPFLESVPRETGGGDDYGLVDNEYFEETVK
                     ILTESLKITRFISETYKRISGGDNVAEKLLVMFCYNVEKGIFDKGISNPMIERFREYE
                     KIVARTRKIAELENRCIHIPSAWAYPKPKATQILCKNKVLECPNYVSGWDINVVTAQE
                     AVGYVGTALKPGAYPKCKLEKELTELKG"
     gene            complement(5345..6304)
                     /locus_tag="PLF66_17220"
     CDS             complement(5345..6304)
                     /locus_tag="PLF66_17220"
                     /inference="COORDINATES: protein motif:HMM:NF020032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65835.1"
                     /translation="MKLGIITNTLSTRNDELTTHDIAFAALQRGHSVYMMSVYDLYSD
                     GKTVYGNVRVIHKKGIDTRVDLTRNLKNFSEEKFRLNDLDVLMLRHKHETGDKGETFH
                     KTAREYAFHLQEAGVLVFNDPRYLPFVSSKLSTLSLDNSILPPHQLISGNFEDLYKYC
                     KDVLKYEGVIKPLGGKGGEDIYFTEKRNLRNNLKSLLKQGPVLIQSYIPNNGDKRILL
                     LDGEPIAWYLRVANDGEFLNNIHAGGKPVKCDLTDRDKKIIEVVKPKLKKYGILFAGI
                     DILGNYLSEMNTENPGGTVRADKLGNFNSKEKIIDFLEKRIKK"
     gene            complement(6371..7300)
                     /locus_tag="PLF66_17225"
     CDS             complement(6371..7300)
                     /locus_tag="PLF66_17225"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008590070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HMZ65836.1"
                     /translation="MQTIVSSDIEKASLVLKNGGVLVFPTETVYGIGADSRNLSACER
                     IYSIKNRPKDNPFIVHVDSIESMGKIAVIPSHFKDAIERLTPGPITFIFEKKDESIFS
                     TGLSTIGVRIPSHPIARKLLEISKTPVSAPSANLSGRPSITRFKDAVSTLINKVDMIL
                     EGEDSEIGVESTVVDLTLSRPVLLRPGGISFTHLNQFFPNLQKYQESKEKNAKPASPG
                     MKYRHYAPDAEVVLISPEEKIEMESAAFIGFKEQSVKFQRIVKTNLEYMHHLYSFFID
                     CDQRGFSRIYCSFPLTDEFEDVLLNRIYKSAMK"
     gene            complement(7310..8947)
                     /locus_tag="PLF66_17230"
     CDS             complement(7310..8947)
                     /locus_tag="PLF66_17230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004499697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HMZ65837.1"
                     /translation="MKLVLIVTFWVLLSGVLFFIGYNLLDNSAVTGPDGKDGKTTQSV
                     GDANGNSKKPEDKKPEAKETKEAEKKTEQEATNKDSFLDSIVSFFKIKSDSAPENSTP
                     EATKAEEPKDLPPIVKEIDKAEFRSSTWFSDYEAMKKNVIYYNEIHPFIYLMKGGLTN
                     TGEISSSWSKTSRKERVAELRALNPKVKIIPTIFRWENPKEKISENIGMNGRNDIRDK
                     HIKNIIEEVETYGYDGIDIDYEGMTCNKKDKFEEFIVLLSKEMKARNKILSVAVHPKT
                     SAKKTKEAKCKGLSHTITQDFAENWRGPMTHDYAFLAKHVDRVKIMAYELHPRKYRNP
                     GPGPQAPNVWLKEIIEYSLARIPSEKLYMAIPTYGYDWALNCNARAKAVYFDTALKIK
                     ASKSIHYQPTDISKIFAENSRSKSWRNLSKFMYIHENKVYEDPSLWYSSEGCDRVAFY
                     MNRKAFEDKMTLLRKYKLAGFSFWQLLSDNDPEINEYLSLLVTNKLPPVEKIDFNPNQ
                     KAEEEKLKEQDPSLQKTNAKNAKSGKHIDKTSMLHKK"
     gene            9237..10499
                     /locus_tag="PLF66_17235"
     CDS             9237..10499
                     /locus_tag="PLF66_17235"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HMZ65838.1"
                     /translation="MPHSLKYHIQAHFKKLPWKVFFFNALISLLITFLHWLSFNFQSN
                     FWIGVTARLFYIPVILASIYGGLLIGTLTGFTSGLLHALLMFSLFQSKSIHHELFLSH
                     IMETVALIFIGFLTGGLKDHEEYERKMREEITGLFDRYVSPVISKQIVENHIPLDGEE
                     KLATVLFCDLADFTSLSERVPPKELLKILNQFFPHIVEILMSKNAFLDKFIGDATMAA
                     FGIPFSSESDAALAVDASIEIQNKLLELNKTNSFGNNNLKVTIGMNSGNVIAGNVGSH
                     KRISYTILGDAVNLASRIQSLNKLYMTNILITDNTLKLINNQRIFKYREIDSVRVKGK
                     KNPTVIYDVYSADSEEMISMKDITLSDFLNGIFHYKMAEFDKAEKIFKKIHKNYPDKI
                     IQIYLDRIRILKKNTPTDWDGVFDFTSK"
     gene            10644..11264
                     /locus_tag="PLF66_17240"
     CDS             10644..11264
                     /locus_tag="PLF66_17240"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016547441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HMZ65839.1"
                     /translation="MEDIRHIIRSRSEERLRNLIEERLKPGANKELIDKKIWNLFGEN
                     WAIIFTDLSGFSRNTAEFGIIHFLQIIFESERILLPIIYEHNGLLIKAEGDSLMLIFR
                     TVVDAVECSVKMQRTLKEYNENKLEHEKVLLCVGIGYGKILRIGDADIFGEEVNAASK
                     LGEDTAKRGEILLTKSAVAELGIQSSYVFHPASEKEDWVEAYKLEY"
     gene            complement(11359..11730)
                     /locus_tag="PLF66_17245"
     CDS             complement(11359..11730)
                     /locus_tag="PLF66_17245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65840.1"
                     /translation="MTSKKKKSLPKEKPNLFEKSIQSFTILGPVENSNIRKIESEEGL
                     PFHLIVAFQKAKAESTKGANLKSNSIDHSVFAVYATSRSKHPSHYIVMGSVAGVNYTY
                     VNLIGVSPDGKKIGKSQMVGD"
     gene            complement(11738..12265)
                     /locus_tag="PLF66_17250"
     CDS             complement(11738..12265)
                     /locus_tag="PLF66_17250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65841.1"
                     /translation="MNKFFLLLVSFFLISADTIEKYQGDISPKEKRIRQYKEEIADLS
                     YSFPEQLKLYFKKTYSDYAVFYDRNGDEVYYRYRRNQFDSEGDRKIIGLFSGQAYRVR
                     GEYSGVARFTDAVNGNLLPFPVLYLKTESDRFWSYDIVEKYDVLKLEHLKDRNTIPVY
                     KLISFESTGIDELIY"
     gene            complement(12277..13596)
                     /locus_tag="PLF66_17255"
     CDS             complement(12277..13596)
                     /locus_tag="PLF66_17255"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HMZ65842.1"
                     /translation="MNSIKKSSNWKEKTRLLSLPTIQNSIFVFVFLFHFSLFSLDVKK
                     NDSPGSDVTPSKVETNKSPLDLDETAEGLRDVEKSGGSDSRKKIKKHSLTPEQLKKKK
                     EVIEKVLKYGSNKERKEAMRELSHLTSEGIDDLIKIVSDTLANDSDNGIKVSCLRTLA
                     ELEAKSESKNIVEAMKNKSDDVKDAAIHAIQKLKVEEASEELNSFLKNQDFTKSSTLV
                     SATINTLSELETGKSSSEFLENKFRDKTTSLDLRSSIALYFGKIKDNKAESALLDVST
                     DDSEDITLRSYAVNALGKMHSPRAVSELRTMLSKINESKSKADIKKYSGLKLYVISAL
                     IMLGDKDILKDLIVYAKDDDPAVRLRAIKQLAEVPDPTVIELVEYKAQRDPNRKVQEL
                     AKKILEEWKQKGVVDTKGQMKNSTSNPDSTSQPIKDKNENLVIPANR"
     gene            complement(13523..14026)
                     /locus_tag="PLF66_17260"
     CDS             complement(13523..14026)
                     /locus_tag="PLF66_17260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010575021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HMZ65843.1"
                     /translation="MVKPKGYFICVDDEVSVLETLREQLLQRFGDTHYIEVADSAESA
                     LALIDEIHNSGEVIELIISDQVMPNMKGDRFLEEIHKKLPDAIKILLTGQAGLDSAIR
                     AINHGGLSRYVEKPWDIDMLSEDINELTTKFRQNLENLHMLNELNKKILELERENEAL
                     KSSHDSE"
     gene            complement(14167..14847)
                     /locus_tag="PLF66_17265"
     CDS             complement(14167..14847)
                     /locus_tag="PLF66_17265"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HMZ65844.1"
                     /translation="MLEMLKEVNEFIDKRETDGNLDKAINKLLELKASNPDMDIICGK
                     LSSAYFYKGLFETANSKKQELYYEHGVNYGKEAITMNPKAIYGNFWYASNVGMLGLCR
                     GMMASLSSIDPMKRSYEIVLKENEKFFFGGPHRALGRIYHQAPGWPISVGNKTKALQH
                     LEKAVELGPEFFNNRLYLAEIYIDIGQKAKAKEQLEWMMNREPKPEHRIEDIGYKEKG
                     IEILGKYF"
     gene            complement(14859..17378)
                     /locus_tag="PLF66_17270"
     CDS             complement(14859..17378)
                     /locus_tag="PLF66_17270"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002768989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HMZ65845.1"
                     /translation="MISNNNDKLWDLMDREPIINKENLERAFAHHLEYTVGKHRNNTK
                     KQDIYEALSFTVRDIIVDRLNETQSNYRAKNPKRVYYLSLEFLIGRTLSNAMINLGIQ
                     NLLEEVLADFGYDLHEIVEYEPDAGLGNGGLGRLAACFMDSLSTLNLPGYGYGIRYEY
                     GIFNQHIENGAQIEKPDAWLHKSGNPWEIIRHEVVYPVHFYGHVETRIDPTGKLMSVW
                     IPQETIIAEAHDYSVPGYNTTTVNNLRLWVAKASEEFNFDYFNHGDYLKAVEDKQKTE
                     NISKVLYPNDTTEQGKILRLKQQYFMVASSLLDIINRYKRFNTDFDRFPDEVAIQLND
                     THPSIAIPELMRILMDEEKLEWGKAWDITTKVFAYTNHTVLPEALETWKVSLIEFLLP
                     RHAQIINEINYRFLNEARSTGKLNDQEITQVSVFHEGPDKLLRMANLSVIGSHSVNGV
                     AALHSELLKTLVFPGFYKLWPEKFNNKTNGITQRRFLLKANTQLSSLIASKIGDGFAT
                     DLKQLRGLEAYATDPDFESEWRRIKKANKERLAKIILNDTGIVVDVNSIFDVQIKRFH
                     EYKRQLLNILHVIALYIRIQENPYKEIVPRTFIFAGKAAPGYYMAKLIIRFINAVGNI
                     VNNDPKVNGRIKVVFLPNYRVSLAENVFPASDLSEQISTAGTEASGTGNMKFALNGAL
                     TIGTLDGANVEIMEEVGSENIYIFGLKAHEVLDIKSRGYHPMEYIERSNELQRIMTLI
                     KENFFCMSNANLFRPIYDNLVHQDNYLLMADFDSYSACQRVVSSEFKDQNLWTKKSIL
                     NVARIGKFSSDRTIQEYADEIWNVKPVEVKTPPLKYTIHRK"
     gene            17604..19235
                     /locus_tag="PLF66_17275"
     CDS             17604..19235
                     /locus_tag="PLF66_17275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004477907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65846.1"
                     /translation="MKKLLLLLLVALWSPALFAQVWTPPGVNSRPTSDTITFDFGINQ
                     YNKDTYLYLAPGLNMNFNNKWGFSMQLPVNLLLYDAEPKTPGSKIGQVRSIDYDSKSD
                     YQRILNNFWIGNFGVYKPKEITYSLFLGRMYDGYIGHGTIVNRYVNNQRIDVYKLGVM
                     ADINTDYGGVQVFTNSIYDRKEVNAGRAYIRPFGIITGIMDLFSGKKNVAMLVANGNV
                     FDDVGRKKVNEEINSNEEVERYVEVETDPKTGEKKEVEKTAPKKERNLEKEETPPTNK
                     IFDLNRFALGFTSAYDGTAPTALDVDTTGSIRFDKDNNAKVKSTKRQGIEGYDAEFKI
                     FSTDTFELTPYADYNRIKNVQNAIGRHYGIIAKIGPRDINVTIRPEYRVMSANYIPMY
                     FDSFYEIERYQANLDTNFPYTKYEFLQNKPVDGPTVRGYYNTLIVNIYRVGFEANYED
                     YTGKNNSRVFLGAYIPFGTLFRLSAFYTKKGFDKSKEAFKLDDKAQGAIEVAINLGPI
                     TIKLQDRRRWVLDSTTNQFTAKDERMILFSGGTAF"
     gene            19460..>23620
                     /locus_tag="PLF66_17280"
     CDS             19460..>23620
                     /locus_tag="PLF66_17280"
                     /inference="COORDINATES: protein motif:HMM:NF014427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HMZ65847.1"
                     /translation="MRIKIFLVITSLVFAIGCSKKEKKYLPFSMNNSVNAPNANASTN
                     VTGATLSQIRVSPETTSIALNTHANFTATGVYSDGTTKDITQDCDWSVKTETDKVSNV
                     SSKKGRFLGSSATSSPATISAASGSISGDANLTVTNATLQSIQISSVPNLTPGGSVQY
                     IATGIFSDGTTQDVSSLVTWSSSNSSSVTINPETGVGTGGSSVGMSTITATLTSPSTF
                     PSVSNGGSSTTNTALTSVAVSSITVTGAATVGAGLTTNYYASVLYTDGTSSDITTQAT
                     WSTANTTTASVNNNTGSKGIVTGIAAGTNTVQVSYLGLTGSKNITVSASLTVSSVSVS
                     PSSKSFAKGTTTQFTATATYSDGSTADVSNSAVWSSSNNSIATVNTSVTTGGLAQAVA
                     VGSATITASTGGKSGTASATVTAAVLQSIAIDTNASVAKGTTKQFTAIGTYSDGSTLD
                     ITTSVTWSSSSTPNVTVSNTTGTKGLATGVAMGSSTIQATLSGITSNSSTLTITAATL
                     SFIAIGGGVSIDNGASNQYSATGTYSDGSTQDLTSLVTWNSASTSVATISNAVADKGI
                     AYGIDVGTSGITATLANGVNGIVTGNGGTVTSNTSTLTVNVPNTGVADTTAPYLAIAT
                     DITASGANVSTILVKFSESVQSGGGVNAADLAANYTVSSATIGSVVKVTSTTYKLVLT
                     SLLTSGTQYTITADNGSFSGGQAIKDIAGNSLSSPNVLTFIADDEMRIIQAEASSTSQ
                     IKIYYSRLVASSGTGSGACADSSSCAAKYYINPSLGTISSVSVSDNVATFTISGSTMR
                     GIAYTIIAANGIQDDPSTAPAGFDTGSTDAITDLAGSKTLVGNPKDRISFTGKGTAVN
                     DFADGWYMSDPFADGSVFAFTFKFKSKVYVGPNMYNNTTLRFDTDGSNVVSNTYKMKS
                     ATWKSLTINCPSTTGFGYGFDGSNNNSCDRDGSGVRYSGGIYEEGLVGYTSLNLTGGD
                     ILAVGPMINNATNAYVTQDTDNQLDMMAFALRSFTGGGNTKSIQAYFSDANNLYMGHA
                     SDHGTQAPILVRMPVTFSSGNITNIDDANNNSNNLNLDATPVGKPTGTGAGYVNAADA
                     IGVDSFTSVTFSGQAKRIYIGNSVGIASSTDLTAFGSAVTSEPAVMKTLGQTNLVLPG
                     IKSGGSYPEGLTKVSPGQRGVPFLKVHNGKLYAARNVSLNSSHSNGAITEGNKTPNYA
                     EVYVCSTPSAGTTGNKYCNATDWSLVFTTNSAVSNGTRFDSSKSDNIAISMFEISPTG
                     MVYLGFDSPSGFRVYRNAVGTSSDITNTGWTQEGPSGSKEGFGDATYNRIQSSVVVQD
                     KNSVYYTYITVGGYDTTNLWHKPIQVYRQADDGTARVALGDFGDSSLMAYISKTASSD
                     SARAAAAAI"
BASE COUNT         7534 a         4281 c         3990 g         7815 t
ORIGIN      
        1 atagtagatc attttaaaaa actaaattct gattttggtc aagaagcagg agatttaatt
       61 ttaaactcac ttgggatcat agcaaaagaa catttttcaa aggaaaattt actctttcga
      121 ttagaaggga aaaagtttgg agtgattctc ccaaatacaa ctagagataa agcattactc
      181 ctactagaca gttttcgaga taaagttgca agcatcccaa tcaactcaaa tggaaacaga
      241 gttgagtata caatttcaat aggactctct gtctctgaaa atcagagttt tgatacaaag
      301 ctgagagaag cagaagaaga attgagccaa gctaggaaaa acggtagaaa ccaagtttct
      361 gcgaaatcct aaatgaatag aatcttaatt attggagcaa ctggtagggt tgggcaaaga
      421 ctcgttcaat actccttgaa tagcaattac aaagtaacag cccttgtaag ggataaatca
      481 aaattaaaat ctacaaatcc aaatttagaa gttatagtag gaagtgtctt agatcctcaa
      541 ataattgaag aagcaatcca aggaaaggaa tttgttttaa cagcgttgag tggaagatct
      601 accaagccag actattcagt attatcgaaa ggaatcgaaa atgttgttac ctgtatggaa
      661 aaagcaaaag taaaaagact tatcagcgtg gcaggagctg ggattttaga tgactcagag
      721 tttggtctta gaaggaatcg acccaactat ccagaaattt ttaaactcgt ttccgcagag
      781 aattggaaaa tttatgaata tttaaaaaca aaagaaatca attggacatt agtatgcgcc
      841 cctgaaatgc cagaagagaa aagaactgga gtctaccgag cgacaaacga ctatttgcct
      901 cttggaggaa gaagaatatc tgttgaagat gttgctgatt atattctaaa aatcttagat
      961 gaaccggaga catttaaaaa aaggattggt attagttact aatcgctaat tggatttaaa
     1021 aagttccgaa cttgtttatc gttctcatac tctgccaaat ataaaatatc tctataatcc
     1081 agctttgtca tttccaagta ctgtctaaat aaatcctcat tcgcagcagt gagaaatagt
     1141 atagaacgaa taattctatc tcttgattcc tttacatcca tttcatctag gtattgattg
     1201 agcctttcta aatgatctaa aattgtatct gtatctaaaa attttttttc gataaaatct
     1261 aaaatatctt ttggtaaatt catttacttt aacttaccaa atttctcttc taaggaagaa
     1321 cctttccttt ttacaaataa attctttagg tcttcatcaa aacgaacttt aaattcatca
     1381 atttctttat cggattggat tagataaggt gtaacatctg tcttagattc aaatgaatac
     1441 ttaccaatat tgccaagggt tggatcattg atgcggggct tactcttatt atccacacat
     1501 tgttcggatg atcctaagtc ataacgaaaa cttccatctc tgtagaactg aaaaaatcca
     1561 cagttcgatg gattggtata acttaggttt agagcacgct tccccggaaa agatctacag
     1621 gaaataaaaa tcaaacaaaa tagaattata agtcgcatta gtttacaatc tttaattttt
     1681 cctgtgcatt gattcttttc acaacatcat tgagttgttt taaaaaatcc ttggtatcac
     1741 cagaacttaa tgtaaaatta tgtgtttctt ttcgatttga atagctaaaa gaaaaattat
     1801 ttttagctga gttcattttt gaaatcagat ctagattgat ttcggattca atttttagaa
     1861 tgtcactata atctgttgaa acttttttta agttataata aactccatct aaataaacgg
     1921 agatccccac aggtaagtct gtatctaaca tatcgagatg atagtctaaa aaatattttt
     1981 tctcacctgt tgaattgtta taaattcctt taattaaaaa actctttgcc ctaccttgtg
     2041 tgcaaaaagg ccaataccaa gtaaagactg tgcatacagg ttctgattta acagttactt
     2101 ggtctaggtt tggttctgga atcacttgaa cactactgca ttgaaaaaga atgagtgtaa
     2161 aaaaaatata aacaaaaata aaaccttttt tcatttctta ctaaactccc acaaattgaa
     2221 actaccagaa aatctatact ttataatcct aaaaattttc ccatttttaa attaataacc
     2281 gctatgaaaa aatcaattac aagaatagta agtatagaaa attagtaatt atgatttcat
     2341 ttccaaaaga aaaaataaac gtattactcc ttgagaacat tcatgaaaat gcatttaatc
     2401 ttttcaagaa tgatggtttt tctgtaaagc ttatgaaaga tgcctactta gaagatgatt
     2461 tatgcgaggc aattaaagat gttcatattc taggaattag aagtaaaaca aatgtaacag
     2521 aaaaagtttt tcagaatgca aataaactac tcactctcgg ttgtttttgt attggaacaa
     2581 accaagtaga tttatctgtt gcagaaaaaa aaggcatacc ggtcttcaat gctccttata
     2641 gcaatacacg tagcgtggca gaacttgtaa ttgctgaaat catttttctc gcaagaaaga
     2701 cgggagacca atcaagagat gctcacaatg gcaaatggaa taagatcgca acaggatgct
     2761 ttgaagtaag aggaaagact ttaggtattg taggttatgg tcatattgga tctcaagttt
     2821 ctattctagc tgaatccttt ggtatgaaag ttatattcta tgatattcaa acaaagcttc
     2881 cactcggaaa tgcgcaaccc gttgatacat atgaaggcgt tttaaaagaa gcagattttt
     2941 taactttcca tgttccagaa agccctgata cgatgaatct tctcggtgca aaagaaatcg
     3001 caaaaatgaa aaagggttct tatttattaa acctatctag aggaagggtt gtagatattg
     3061 atgcgttagc tgctgccttg aagtctggtc atttagcggg agctggaatt gatgtttttc
     3121 cagaagaacc aaaatcaaat aaagatccat ttgtaagtcc acttcaaaat attccaaacg
     3181 taattctaac tcctcatata ggaggaagca cagaagaagc acaaagaaat attggactag
     3241 aagttgcgaa taaactactt cgctatataa ataacggttc tacatctttc gctgttaact
     3301 tcccaaatgt ggaattgcca cttttaaaag acaatcatcg aattttaaat gttcataaaa
     3361 atcaacctgg cttcttgaaa gatatcaaca aagtggtatc agatctagga gcaaatatta
     3421 cagctcaatt cttatctact acagcagata taggttactt gattatggat attaacaaac
     3481 aattaggtga tcaagtaaaa gaggaaattg agaaaaatcc tttgagtata aaaacaagaa
     3541 ttttatacta aaaacagatt taccagaaat taagtaattg gggattttct gtatagattt
     3601 agcaactttc ttgctgttgc ctttctattt cagaaagtct ctgacacgtt agccgctcga
     3661 agttccaaga atattagaaa agactagcaa ttgaattaga actcactgaa tacaaaaaaa
     3721 aggaatgtag taaaactata tattcctttt tctctactga atgttggatt aagattcttt
     3781 taccgaacac caaaagaaaa gttccatttt aaaaataaga atgtttcgtc taaagacttc
     3841 ttagtgtcga atgcgttcgc atgcgcagta taccaaatgc gactcaaaaa tatttctaga
     3901 ttatttttga catttggtat acttactctt tagattgaat tatcccttta gctctgtaag
     3961 ctctttctct aatttacatt ttggataagc cccaggtttt aaagctgttc caacatagcc
     4021 aacagcttct tgcgcagtta ccacattgat gtcccatcca cttacatagt ttggacattc
     4081 tagaacttta tttttacata aaatctgtgt tgcttttggt tttggataag cccaagcaga
     4141 agggatatga atacatctat tttctagctc tgcaatcttt cttgttctgg ctacaatttt
     4201 ttcatattct ctaaatcttt cgatcattgg gttggatata cctttatcaa agattccttt
     4261 ttcaacgtta tagcaaaaca taacgagtaa tttttctgct acattgtcgc caccactgat
     4321 tcgtttgtaa gtctcactga taaaacgtgt gatttttaaa gactctgtta gaatttttac
     4381 agtttcttca aaatattcat tgtccaccag accataatca tctccccctc ctgtttctct
     4441 aggcacactt tctaaaaatg gatttgcatt tagaacatta aaatccattg gatctggttt
     4501 atccactaca tagacacctt ttacaatatg attttgaatg gctgatacga tttgtgcagg
     4561 catatttaca ttttgtgcaa tctgtgcatt tagctttgcc agtctctcca tgagagtatc
     4621 atctaagtta gattttctcc aatagtctgt gttaaagaat ataacgtccg aaaacatgcc
     4681 tgttaagaat gcataacggt gaatgagttt aatattatag cttctttgga caacagttcc
     4741 taagcaaata agtcccatgc tcacaatatg ctgaaagaaa tcatactgct ctaacataat
     4801 tttatataaa acaagaacaa ggtcgtttgt ataaaaagaa ctattaatta aactctcagt
     4861 gcttacaaca ctcgattgat cattgttata aagaatagat ataaattcat tttttgattt
     4921 ttgaatgcct ggcatgatct cagcggttaa cgctttctta atacgtccag ttacatccga
     4981 agtcatattc actttaaact ttgactcata ttgtcccgaa agagattcca acttttttaa
     5041 tgcagattct tttatatgaa cattgtcttt gattaaaata ttaccttttt tatctcggat
     5101 ggattctctg agttgcacaa ttccaagtaa atctagatca tttacaaatt ttgtaaattc
     5161 tcttaaatca tcaaattcta tacctgctag agtaattttc attttttctc ctttttattt
     5221 gtaaaatagc aaaagatagc atatagatca tttttgtttc gaataaaact ctaaatattc
     5281 cctagtccta atttaaatgt tccattaggg aaatttaatc tatactcttc tttataatat
     5341 taaattactt tttgatcctc ttttctaaaa aatctatgat tttttctttc gaattaaaat
     5401 taccaagctt atctgcacga acagttccac ctggattttc tgtattcatt tcacttagat
     5461 aatttccgag tatatcaata cccgcaaaca atattccata ttttttcaat ttgggtttta
     5521 ctacttcaat aattttttta tctctatccg ttaaatcaca ttttacaggt tttccacctg
     5581 catgaatatt atttaaaaac tcaccatcat ttgcaacacg caaataccaa gcgataggct
     5641 ctccatctaa aagtaagatt cttttgtctc cattattagg aatatagctt tggattaaaa
     5701 caggtccttg ttttagaaga gatttgagat tgtttcgtaa atttctcttt tcagtaaaat
     5761 agatatcttc tccacctttt ccaccgagtg gtttaatgac tccttcatat tttaaaacat
     5821 ctttacaata tttgtaaaga tcttcaaaat ttccagatat caactgatgt ggcggcaaaa
     5881 tactgttatc caaactaagc gtagataatt tcgaagaaac aaaaggcaaa tacctcggat
     5941 cattaaagac taaaactcct gcttcttgca aatgaaatgc gtattcacgg gctgttttat
     6001 gaaaggtttc ccctttgtcc cctgtttcat gtttatggcg aagcattaat acgtctaaat
     6061 cattgagcct aaacttttct tcagaaaaat ttttaagatt acgggtgaga tcaactctag
     6121 tatcaatccc ctttttatga atcacgcgca cattgccgta gacggttttc ccatcggaat
     6181 agagatcata aacagacatc atatagacac tatgacctcg ttgcaatgca gcaaacgcaa
     6241 tatcatgagt agtcaattca tcatttcgag tggaaagtgt attcgtgatg attcctagct
     6301 tcataaatcc gaattcaaat ttaaattgag attccttgag ttacaataat tttttatttt
     6361 tatcagaaaa ttatttcatt gcagatttat aaattcgatt gagtaaaaca tcttcaaact
     6421 catctgtaag tggaaaagaa caatagattc tactaaaacc tctttggtca caatcaataa
     6481 aaaaagagta gagatgatgc atatattcta aattggtttt tactatacgc tgaaatttta
     6541 cactttgttc cttaaatcct ataaacgcag cagattccat ttctattttc tcttcaggac
     6601 taattaggac aacttccgca tcgggagcat aatgcctata tttcatacct ggactagctg
     6661 gttttgcatt cttttccttg ctctcttggt atttctgaag atttggaaaa aactgattca
     6721 aatgagtgaa agaaattcct ccaggacgaa gtaagactgg tctagaaagc gttaagtcta
     6781 cgactgtaga ctctacacca atttcagagt cttccccttc taagatcata tcaaccttat
     6841 tgattagggt agaaactgca tctttgaatc gagtaatcga tggtcgtcct gataaattcg
     6901 ccgagggagc tgatactgga gtcttggata tctctaataa tttcctcgca atcggatggg
     6961 aaggaattct gactccaata gtagaaagac ctgttgaaaa tatggattca tcttttttct
     7021 cgaaaataaa tgtaatcggt cctggggtca atctttcaat cgcatcttta aaatgagagg
     7081 gaataacagc gattttcccc attgattcaa ttgaatctac atgaactata aaaggattat
     7141 ctttaggacg gtttttgatt gagtaaattc tttcacaggc ggaaagattt ctggaatcag
     7201 ctccaattcc atagactgtt tctgttggaa aaacaaggac tcctccgttc tttagaacaa
     7261 gagaagcctt ttctatgtca gagctgacaa ttgtttgcaa ctatcgactt tattttttat
     7321 gcaacatact ggttttatct atgtgcttac cagattttgc atttttagca ttggtctttt
     7381 gaagagatgg atcttgctct tttaacttct cttcttctgc tttttggttt ggattaaaat
     7441 ctattttctc tacaggtggt aacttatttg taacaagtag agacaaatat tcattaatct
     7501 ctggatcatt atccgaaagc aattgccaaa aagagaaacc agctaactta tattttctga
     7561 gaagagtcat tttatcttcg aaagctttac ggttcatata aaaagctaca cgatcacaac
     7621 cctctgaact ataccagaga cttggatctt catagacttt attttcgtga atgtacataa
     7681 acttagaaag atttctccag ctttttgaac gactattttc agcaaaaatc ttggagatat
     7741 cagttggttg gtagtgaatt gacttagatg ctttgatctt gagcgctgta tcaaaataaa
     7801 ctgctttagc tcttgcgtta caatttagcg cccagtcgta tccataagtt ggaatcgcca
     7861 tgtataattt ttcagatgga attctagcta aagaatattc aatgatttct tttagccata
     7921 cgttaggtgc ttgtggtcca ggtcctggat tacgatattt acgagggtgt aattcatacg
     7981 ccatgatctt tactcgatct acatgttttg ctaagaaagc ataatcatgt gtcattggtc
     8041 cacgccaatt ttctgcaaag tcctgagtaa ttgtatgaga tagaccttta catttagctt
     8101 ctttggtctt cttagcagat gtttttggat gaactgcaac cgataaaatt ttatttcttg
     8161 ctttcatctc ttttgaaaga agaacaataa attcttcgaa cttgtctttt ttgttacaag
     8221 tcattccttc atagtcaata tcaatcccat catatccata tgtttctact tcttctatga
     8281 tgtttttgat atgtttgtcg cggatatcgt ttctaccatt cattccaatg ttttcagaaa
     8341 ttttttcttt tggattttcc caacggaaaa tggttggaat gatttttact tttggattga
     8401 gcgctcttaa ttctgctact ctttcttttc tagaagtttt actccaagaa gaggaaattt
     8461 cccctgtgtt tgtgagccca cctttcatta ggtagataaa agggtgaatc tcattgtaat
     8521 agatcacatt tttcttcata gcctcgtaat cagaaaacca ggtagaagat ctaaattctg
     8581 ctttatcaat ttctttgact atagggggaa gatcctttgg ctcttctgct ttggttgctt
     8641 ctggggtaga attctctggt gcagagtcag attttatttt aaaaaaagaa acaattgaat
     8701 ctaagaaact gtccttattt gtagcttctt gctcagtttt cttctcggct tcttttgttt
     8761 ctttagcttc tggcttttta tcttctggtt tcttagaatt gccatttgcg tccccaacag
     8821 attgggttgt tttaccatct ttaccatctg gaccagtcac tgctgagtta tctaatagat
     8881 tgtatccaat aaagaataaa actcctgaaa ggagaaccca gaaagtcacg attaaaacta
     8941 atttcatctg acttccaaat ttttgaaata tcttattcaa ttacgccttc cttcttactt
     9001 agtaagtaga cactaaaaaa agaggttttt aacccctttc ctgtcatttt ttccgaccta
     9061 ggatttctat aaaccccttt taggacgatt ggggcaaaag gaaatcacag aaaatcccta
     9121 ttttcaagtc ttttttctgt ctatagtatc ggtgcaatgg acgaaatatt tttctggttg
     9181 atttaaatgt tcgttttgaa tctcagtatc gaatagatta aattcaatcc agccgtatgc
     9241 cgcatagttt aaaatatcac attcaagctc atttcaaaaa actaccctgg aaagttttct
     9301 ttttcaatgc tctaatttcc cttctgatta ccttcctaca ttggcttagt tttaactttc
     9361 aaagcaattt ctggattgga gtcaccgctc gtttatttta tataccagtg atccttgctt
     9421 ctatctatgg aggacttttg ataggaactt taacaggttt cacatccgga ctactccatg
     9481 ctctcttgat gttttccctt ttccagtcta agtcgatcca tcatgaattg tttttgagtc
     9541 atatcatgga aacagttgca ttgattttta ttggctttct cacaggcggc ttaaaagacc
     9601 atgaggaata tgaaagaaaa atgagagaag aaattactgg attgtttgat agatatgtct
     9661 ctcctgttat tagtaaacaa attgtagaaa atcatatccc gctcgatggt gaggaaaaat
     9721 tagcaaccgt attattctgt gaccttgctg atttcacatc gctctcggaa agagtcccac
     9781 ccaaagagtt attaaaaatt ctaaatcaat ttttcccgca cattgtagag attctaatga
     9841 gtaaaaatgc atttctagat aaatttattg gagatgcgac gatggcggca tttgggattc
     9901 ctttttcgag tgaatctgat gcagctttag cggttgacgc ttctattgaa attcaaaata
     9961 aacttttaga attaaacaaa acaaatagct ttggaaacaa caatttaaaa gttacgattg
    10021 gaatgaactc aggaaatgta attgcaggaa atgttggatc acataaaaga atttcttata
    10081 caattctcgg agatgcggtt aatctagctt ctagaataca atctctaaat aaattatata
    10141 tgactaacat cttaattact gacaacactc tcaagttaat aaataatcaa agaattttta
    10201 aatatagaga aatagattct gtgcgtgtga aaggcaaaaa aaatccaaca gtaatttatg
    10261 atgtctattc agctgattca gaagaaatga tttcaatgaa ggatataacg ttaagcgact
    10321 tcctgaatgg aatctttcat tataaaatgg ccgagtttga taaagcagaa aaaatcttca
    10381 aaaaaattca taaaaactac cccgataaaa ttattcaaat ctacctagat cgaattagaa
    10441 ttttaaaaaa gaatacacca acagattggg atggggtttt tgattttacc tctaaatgat
    10501 tttacctcta ttctcattgt ttaggagact ctgagaataa gcgaggttta agttaaatga
    10561 ttgaatttta aaaagaccaa gtttcaaaaa agttctggaa attatttttc aagattgaaa
    10621 tctcagtcta ggcaggaaaa aaaatggaag acattagaca tatcatacgc agtagatcag
    10681 aggaaagact tcgtaattta atcgaggaaa gattaaaacc gggagcgaat aaagaactaa
    10741 tcgataaaaa aatctggaac ttattcggag agaactgggc aataatcttt accgatcttt
    10801 cgggcttttc tagaaacacc gctgaatttg gaatcattca tttcttacaa attatattcg
    10861 agtcagaaag aattttattg ccaattattt atgaacacaa tggtcttcta atcaaggcgg
    10921 aaggagacag ccttatgttg atatttcgca cggtagtcga tgcagttgaa tgttctgtta
    10981 aaatgcaaag aactttaaaa gaatacaacg aaaataagct agaacacgaa aaagtgcttc
    11041 tctgtgtagg aattgggtat ggaaaaatcc tacgaattgg agatgcagat atttttggag
    11101 aagaagtcaa tgcagcaagt aaacttggag aggatactgc aaaaagagga gaaattttac
    11161 tgactaaatc ggcagttgcg gaactaggca ttcaatcttc ttatgttttc cacccagctt
    11221 ccgaaaaaga agattgggta gaagcctata aattagagta ttagtttgat tgaaactatc
    11281 tagtaattat gaaatattca gaattaaaag taaggctttc ttacttttaa taggatatcg
    11341 attgatagaa tttttctttt agtctccaac catttggctt ttaccaatct ttttcccatc
    11401 ggggctaaca ccgattagat tcacataggt ataattcact cctgcaaccg aacccattac
    11461 tatataatgg ctcggatgct ttgatctaga agttgcataa actgcaaaca cagagtgatc
    11521 gattgaattt gattttaaat ttgcaccctt agtgctttct gcttttgctt tttgaaaggc
    11581 tacgatcaaa tgaaaaggta gaccttcctc tgattcaatt tttcttatat tagaattttc
    11641 aacaggtcct aaaattgtaa aagattgaat cgacttttca aataaattag gcttctcctt
    11701 aggaagagat tttttctttt tagatgtcat agaaattcta gtaaattaat tcatcaatac
    11761 ctgtagactc aaaggaaatc aatttgtaaa caggaatggt atttcgatct ttcaaatgtt
    11821 ctaactttaa aacatcatat ttttcaacaa tatcataaga ccaaaatcga tccgattctg
    11881 tttttaaata gagaacggga aatggtagca aattgccatt aaccgcatct gtaaaacgag
    11941 ctacacctga atactcacct cgcacacgat atgcttgtcc tgaaaatagc ccaatgattt
    12001 ttcgatcacc ctctgaatca aattgattcc ttctatatcg ataataaact tcatcgccat
    12061 tcctatcata aaaaactgca tagtcgctgt atgttttttt gaagtataat tttaattgtt
    12121 caggaaatga ataagataga tcagcaattt cttctttgta ttgtctgatt cttttttcct
    12181 ttggggatat atcaccttgg tatttttcaa tcgtatcagc agaaattaaa aagaaactaa
    12241 ctaacaacaa aaagaatttg ttcatgacgc tttctcctat cgatttgctg gaattactaa
    12301 attttcattt ttatctttta taggttggga agtagaatca ggatttgagg ttgaattttt
    12361 catctgtccc tttgtatcta ccacgccttt ttgtttccat tcttctagta ttttcttagc
    12421 tagttcttgc accttacgat ttggatctct ttgtgctttg tattctacta attcaattac
    12481 tgtgggatca ggaacttccg ccaattgttt aatggctctg agtctcaccg cggggtcatc
    12541 atcttttgca tatacaatta aatcttttaa gatgtctttg tctccaagca tgatgagagc
    12601 gctgattaca tatagcttaa gcccagaata ttttttaata tcagctttac tttttgattc
    12661 attaatcttt gaaagcatag ttcgtagttc ggataccgct ctaggagaat gcattttgcc
    12721 aagtgcgttt actgcataag atctgagagt aatatcttca gagtcgtcag tcgaaacatc
    12781 taaaagagca gactcagctt tattatcttt tatttttcca aaatataatg cgatgctaga
    12841 acgtaaatcg agtgatgtag ttttgtctct aaatttgttt tctaaaaact cagaactact
    12901 tttaccagtt tccaactccg aaagtgtatt aatcgttgct gaaactaaag tgctactctt
    12961 tgtaaaatct tgatttttta aaaaagagtt taattcttcg gatgcttctt ctactttgag
    13021 cttttgtatt gcatgtatag ctgcatcttt tacatcatct gatttatttt tcattgcctc
    13081 aacaatattt ttggattcgc tcttggcttc taattctgct aacgttctta gacaagatac
    13141 tttgattcca ttatctgaat catttgccag agtatcggag acaatcttta ttaaatcatc
    13201 gattccttcg gatgtcaaat gcgatagttc tcgcattgct tcttttcgtt ctttattaga
    13261 tccgtatttt aaaacttttt cgataacttc ttttttcttt tttaattgtt ctggagtcag
    13321 actatgtttt tttattttct ttcttgaatc tgatccacct gatttctcga catctcggag
    13381 accttctgcg gtttcatcta aatcgagtgg agatttattt gtttcaactt tagaaggagt
    13441 tacatcactc cccggagagt cgttcttttt tacatctaat gaaaacaggg aaaagtggaa
    13501 taaaaagaca aatacaaaaa tactattctg aatcgtggga agacttaaga gcctcgtttt
    13561 ctctttccaa ttcgaggatt tttttattga gttcattgag catatgtaag ttttccagat
    13621 tctgtctgaa ttttgtggta agttcattta tgtcctcgct gagcatgtct atatcccatg
    13681 gtttctccac ataacgactg agtcctccat gattgatagc tcgtatagca gaatcaagtc
    13741 ctgcctgccc agtaagcaga atcttaattg catcaggtag tttcttatga atctcttcaa
    13801 gaaatctatc ccctttcata tttggcatca cttgatcgga aataatcaat tcgattactt
    13861 ctccactgtt gtgaatttcg tcaatcaaag cgagcgcact ctctgcacta tctgccactt
    13921 ctatataatg agtatccccg aatcgttgta ataattgttc tcgaagtgtt tcaagaaccg
    13981 atacttcatc atcgacacat ataaaataac ctttaggttt aaccatattt gattctgctt
    14041 tgcaattcta ttgaaaatat ttatactgat aagtccattg aaaaatcttg agtctgcttc
    14101 aaattagtaa aaaagatatt gcctaatctt ttacatctca agacttttct ggatttaaag
    14161 tcaagtttag aagtattttc ctaaaatttc aattcctttt tctttgtatc cgatatcctc
    14221 aattctgtgt tctggttttg gctctcggtt catcatccat tctaattgct cttttgcctt
    14281 cgctttttgt cctatatcga tgtagatttc agccaaatag agtcggttat tgaaaaattc
    14341 tggtcctaat tcgactgctt tttccaagtg ttggagtgct tttgttttat ttccaacaga
    14401 aatcggccag cctggagctt ggtgataaat tctaccaagt gctctatgag gaccaccaaa
    14461 gaagaatttt tcattttctt tgagaacgat ttcatagctt cttttcattg ggtcaataga
    14521 agaaagggat gccatcatac ctcggcaaag accaagcatt cccacgttgg aagcatacca
    14581 gaaattccca taaatagctt ttgggttcat ggtaatcgct tcttttccat agttcacacc
    14641 atgttcataa tagagctctt gtttttttga atttgcagtt tcaaataagc ctttataaaa
    14701 ataagcactt gaaagtttac cgcaaataat atccatatct gggtttgatg cttttaattc
    14761 taaaagctta ttaattgcct tatccagatt cccgtctgtt tctcttttat caatgaattc
    14821 attgacttcc tttaacattt ccaacatgtt cacttccttt attttctatg tattgtatat
    14881 tttaatggtg gggttttcac ttctactggt ttcacattcc aaatttcatc tgcatactct
    14941 tgaatggttc gatcagaaga aaactttcca attctcgcaa cattcaaaat agatttttta
    15001 gtccaaaggt tctgatcttt aaattcactc gatacaactc tctgacaagc tgaatacgaa
    15061 tcaaagtctg ccatgagtaa ataattatcc tgatgaacta gattatcata aattggtcta
    15121 aataaatttg cattcgacat acagaaaaag ttttctttga tgagagtcat aattctctgt
    15181 aactcattgc ttctttctat gtattccatt gggtgatatc ctctcgattt aatatctaat
    15241 acttcgtgag ccttgagccc gaagatataa atattttccg atcccacctc ttccataatt
    15301 tcaacgtttg ctccgtctaa cgttccaatg gtcaaggctc catttaacgc aaacttcata
    15361 ttacccgttc cagacgcttc tgttccagca gttgaaattt gttctgaaag atcactcgcg
    15421 gggaaaacat tttcagcaag tgacactcga tagtttggta aaaatacaac tttgattcta
    15481 ccatttacct ttggatcatt atttacaatg ttaccaaccg cattgataaa acgaataata
    15541 agttttgcca tatagtatcc aggagccgcc ttaccagcaa aaataaaagt gcgtggaaca
    15601 atttctttgt atggattctc ttgtattcga atgtataatg caattacatg aagaatattg
    15661 agcaattgtc ttttatattc atgaaaacgt ttaatttgca catcgaaaat tgagtttaca
    15721 tccactacga tgccggtatc atttaaaata atttttgcaa gtctttcttt atttgccttt
    15781 tttatacgtc tccattccga ttcgaaatct ggatctgttg catatgcctc tagtccgcgc
    15841 agctgcttta aatctgtggc aaatccatct ccaatcttac ttgcaatcag agatgaaagc
    15901 tgtgtattcg ctttgagtaa aaatcttctc tgtgtaattc catttgtttt attattaaat
    15961 ttttctggcc aaagcttata gaagccagga aatactaagg tctttaacaa ctctgagtga
    16021 agtgcagcaa ccccatttac actatggcta ccgataacag atagatttgc catgcgaaga
    16081 agtttatcag gtccttcatg aaaaacagat acctgagtga tttcttgatc atttaatttg
    16141 cctgttgaac gtgcttcgtt taaaaagcga tagttgatct cgttgataat ctgtgcatga
    16201 cgaggaagca agaactcaat taagcttact ttccatgtct ctaacgcttc tggcaaaact
    16261 gtgtggttgg tatatgcaaa tacttttgta gttatatccc aagctttacc ccactctaat
    16321 ttttcctcat ccattagaat acgcattaac tcagggatag cgatacttgg gtgcgtatcg
    16381 tttaattgga ttgctacttc atctggaaaa cgatcaaagt ctgtattaaa tcttttgtaa
    16441 cgatttataa tatctaaaag actggaagca accataaagt attgctgctt gaggcgtaaa
    16501 atctttcctt gctcagtcgt atcatttgga tataaaacct tggaaatatt ttctgtcttt
    16561 tgtttatctt ctactgcttt taaataatca ccatggttaa aataatcgaa attaaattct
    16621 tctgaagcct ttgcaaccca cagacgaaga ttatttacag tcgttgtatt gtagccaggc
    16681 accgaataat catgtgcttc tgcgataata gtctcttgtg gaatccaaac agacatgagt
    16741 tttccggttg ggtcaattct agtttccaca tgtccataaa aatgaacagg atacacaacc
    16801 tcatgtcgaa taatttccca aggatttcca cttttatgaa gccaagcatc tggcttttca
    16861 atttgagcgc cattttcgat atgttggtta aagattccat attcatagcg gatgccataa
    16921 ccgtatccag gtagatttaa agtagagaga gaatccatga agcatgcggc taatcgacct
    16981 aaacccccat tccctaatcc tgcatcgggt tcatattcga ctatttcatg taaatcatac
    17041 ccaaaatcag ctaatacttc ttccaataag ttctgaattc caagattaat cattgcattc
    17101 gataaagttc taccgattaa aaattcaaga gataggtaat agacacgttt aggatttttt
    17161 gccctataat tggactgagt ctcattgaga cgatctacaa taatatcgcg aactgtaaag
    17221 ctaagagctt cgtaaatatc ttgctttttt gtattatttc tatgtttacc gaccgtatat
    17281 tctaaatgat gtgcaaatgc tctttcgaga ttttctttat taatgattgg ttctctatcc
    17341 attaaatccc atagtttatc attgttatta ctaatcatca ttacctcttg ttggattttt
    17401 ttaatctctt gatggttgta gaatcaaatt catctagaaa tttttataat tatcagtgta
    17461 attcagtcag cctttcttta acatgcaagt gatttttaat ttttgcaaaa atatttattt
    17521 tttagttgct cagttctttc aaattagtat cgttttaagt cttgctcaaa attttttttg
    17581 tttatacgat tcgcagactg actatgaaaa aacttttatt acttttattg gtagcgcttt
    17641 ggtctccagc cttatttgca caagtatgga ctccaccggg tgtaaatagt agacctacaa
    17701 gcgatacaat cacttttgac tttggtataa atcaatacaa caaagatacc tatctctatc
    17761 ttgcccctgg actcaatatg aattttaata ataagtgggg cttctcgatg cagcttccgg
    17821 tcaatttgct tctatacgac gcagaaccca aaactccggg ctctaaaata ggccaagttc
    17881 gctctatcga ttacgattca aagtcagact atcaaagaat tttaaataat ttttggattg
    17941 gaaacttcgg agtctataaa ccgaaagaaa tcacctactc tctttttcta ggtcgaatgt
    18001 atgatggtta tatcgggcat ggaactattg tgaatcgtta tgtaaacaac caaagaattg
    18061 atgtatataa gttaggagta atggctgaca ttaatacaga ttacggcggt gttcaggtct
    18121 ttacaaattc aatttacgat cgtaaagaag taaacgcagg tcgtgcttat attcgaccct
    18181 tcgggattat tacaggaata atggacctgt tctcaggaaa gaaaaatgta gccatgcttg
    18241 tcgcaaatgg aaatgttttt gatgatgttg gcagaaaaaa agtaaacgaa gaaattaact
    18301 ccaatgagga agtagaacgt tatgtggaag tagagacaga cccaaaaaca ggagaaaaga
    18361 aagaagtaga aaaaactgct cccaaaaaag agcgaaatct agaaaaagag gaaaccccac
    18421 ctacgaataa gatctttgac ttaaacagat ttgcacttgg gtttacttct gcctatgacg
    18481 gaacggctcc cacagcactc gatgtagata caacaggaag cattcgattt gataaagaca
    18541 ataatgcaaa agtaaaatca accaaacgac aaggaatcga aggatatgat gcagaattta
    18601 aaatttttag cactgatact tttgaattaa ctccgtatgc agattacaat cgaatcaaaa
    18661 acgttcaaaa tgctatagga cgacattatg gaatcatagc aaagataggt ccaagagata
    18721 tcaatgtaac aattcgtcca gaatacagag tgatgagtgc aaattatatt ccaatgtatt
    18781 ttgattcttt ttacgaaatc gaaagatacc aagcaaattt agatacaaat ttcccttata
    18841 caaaatatga gtttcttcaa aataaaccag tcgatggtcc aaccgttaga ggatattata
    18901 atactctcat cgtgaatatc tatcgagttg ggtttgaagc aaattatgaa gattatactg
    18961 gtaaaaataa ttccagagtt tttcttggtg cttatatacc atttggaacc ctatttcgct
    19021 tatcggcttt ttatacaaag aaaggttttg ataaatcaaa agaagcattt aaactagatg
    19081 ataaagcgca aggcgcaata gaagtagcaa taaatctagg tcctattact ataaagctac
    19141 aagatagaag acgatgggtt ttagatagca caacaaatca attcactgct aaagacgaga
    19201 ggatgatatt attctctggg ggcacagcat tttaataaaa gaatatcaca gtattatcga
    19261 agattcatat agtaaaaata catcaataag gcaaaattct tggtttattg aaaaatatct
    19321 tttttatagt tgaagctttg tattctttct ttagtctttt cctttatagc aacataacat
    19381 gaatgttttc gtgaatcaat tttcatttag attctatgat ttcattttat gagctgtaaa
    19441 aataaaggag acaaagacta tgagaataaa aatattctta gttataacta gcttagtctt
    19501 cgcgatagga tgttcaaaga aggaaaaaaa gtatttacct ttctcgatga ataattcagt
    19561 aaatgcgcct aacgcgaatg ctagcacaaa tgtaacagga gccacactgt cacaaatccg
    19621 tgtatcacca gaaactacat caattgcttt aaatacacat gcaaatttca ctgcgacagg
    19681 cgtttattca gacgggacaa caaaagacat cacacaagac tgtgattgga gtgtaaaaac
    19741 agaaacagac aaagtatcaa atgttagctc aaaaaaaggt agatttctag gatcatccgc
    19801 gacctcatct cctgcaacta taagtgcagc atccggatct atttcgggcg atgcaaattt
    19861 aaccgttacg aatgcaacac tacaatcgat tcaaatttcc tcggtgccta atttaacacc
    19921 cggtggaagt gttcagtata tcgcaactgg tatcttttca gatggaacaa cacaagacgt
    19981 tagttctttg gtaacttggt cttctagcaa tagttccagt gtaaccatta acccagagac
    20041 tggagtagga acaggtggtt ctagtgtagg tatgtccaca atcactgcaa ctttaacttc
    20101 tccttctact ttcccttctg tttcgaatgg tgggtcaagc acaacaaata ctgcattaac
    20161 aagtgtagcc gtatcttcta ttactgttac tggagcagca actgttggag caggattaac
    20221 tacaaactat tacgcaagtg tgctctatac agatggaaca tcttctgaca tcacaacaca
    20281 agcaacttgg tcaacagcaa acactacaac tgcctctgtg aataacaata ctggtagcaa
    20341 agggattgtg acagggatcg ctgcgggaac aaatactgtt caagtatcct atttaggtct
    20401 cactggatct aaaaatatta cagtatcagc aagtctcact gtatcttctg taagcgtgtc
    20461 tccatctagc aaatcttttg caaagggaac tacaactcaa tttacagcaa cagctactta
    20521 ttcagacggt tcaactgctg atgtatctaa ctcagctgta tggtcttctt ccaataactc
    20581 aattgcaact gtaaatacct ctgtaacaac aggcggtctt gcacaagctg tagcagtcgg
    20641 tagtgcaact atcacagcct ctactggcgg taagtcagga actgcttccg caactgtaac
    20701 tgctgctgtt cttcaatcaa ttgcaattga tacaaatgca tcggttgcca aaggaactac
    20761 aaagcaattt actgcaattg gaacttactc tgatggttcc acattagata taactacttc
    20821 agtaacatgg agttcttctt caacacctaa cgttaccgtt agtaacacaa ctggaacaaa
    20881 aggtctggca acaggtgttg cgatgggatc aagcacaatc caagctacac tcagtggaat
    20941 tacaagcaac agttctactt tgacaataac agctgcaaca ctttctttca ttgcaattgg
    21001 cggtggtgtt tcgattgata atggtgcatc gaatcaatac tcggcaactg gaacttattc
    21061 agatggaagc actcaagact taacctctct tgtaacttgg aattctgcct ctacctctgt
    21121 tgcaacaatc agtaacgcag ttgcggacaa agggattgca tacggtattg atgttggaac
    21181 gtcaggaatt acagcaactt tagcaaatgg cgtaaatgga attgtaacag gaaacggagg
    21241 aacagtaact agtaacacat ctactttaac agttaacgtt ccaaacactg gtgtagcaga
    21301 tacaacagct ccatatttag ctattgcaac cgacattaca gcgtccggag caaatgtttc
    21361 tactatctta gttaagttct ccgaatcagt tcaatctggt ggtggagtaa atgctgctga
    21421 cttagcggca aactatacag taagcagtgc aacaattgga tcagttgtta aagtaacttc
    21481 aactacttat aaacttgtcc ttacatctct acttacttct ggaacacaat atacaataac
    21541 agcggataac ggttcattct ccggtggtca agcaatcaag gatattgcag gcaatagcct
    21601 aagctctcca aacgtgctta catttattgc agacgatgaa atgagaatta tccaagcgga
    21661 agcatcttca acttctcaaa tcaaaattta ctattctaga ttagtagctt cctctggaac
    21721 aggatctggg gcttgtgctg attctagctc ttgtgctgct aaatactaca tcaatccatc
    21781 cctcggaaca atcagtagtg tttccgtttc ggataacgta gcaaccttta caatcagtgg
    21841 atcaacaatg cgtggtatcg cctatactat catagcagcg aatggaatcc aagatgatcc
    21901 tagcacagca cctgctggat ttgatacagg atctacagat gcaattacag acttagcagg
    21961 atctaagaca ttagtaggaa atccaaaaga cagaattagc tttactggaa aaggaactgc
    22021 agttaatgat tttgcggatg gttggtatat gtctgatcca tttgcagatg gttctgtatt
    22081 cgcatttaca ttcaagttta aatctaaagt gtatgttgga ccaaatatgt ataacaacac
    22141 tactctccgt tttgatacag atggaagtaa cgtagtatcc aatacataca aaatgaaatc
    22201 tgctacttgg aagtctttaa caatcaactg tccaagcaca acaggttttg gatatggatt
    22261 tgatggatca aacaacaaca gttgtgatag agatggatcc ggtgttcgat attctggcgg
    22321 tatctatgag gaaggcttag taggatacac ttctcttaac cttacaggtg gagatattct
    22381 tgctgtcggt ccaatgatta acaatgctac taacgcatat gtaacacaag atacagacaa
    22441 ccaattagat atgatggcat tcgctctcag atctttcact ggtggtggaa acacgaagag
    22501 tatccaagca tacttctcag atgcaaacaa cctttatatg ggacatgcat ccgatcacgg
    22561 aacacaagct cctatcttag ttagaatgcc agtgacattc tcttctggaa atatcacgaa
    22621 tattgatgat gcaaacaata actcaaacaa cttaaaccta gatgcaacac ctgttggtaa
    22681 acctactgga actggagctg gatatgtgaa cgctgccgat gcaatcgggg tagattcttt
    22741 cacatccgta acattcagtg gtcaagcaaa acgtatctac attggaaaca gtgttggtat
    22801 tgcttcttcg actgacctaa ctgcattcgg ttctgctgtt acatctgagc ctgcagttat
    22861 gaaaacactc ggacaaacaa acctagtatt accaggtatc aagtctggag gttcttatcc
    22921 agaaggttta acaaaggtaa gcccaggaca aagaggggtt ccattcctaa aagttcacaa
    22981 tggtaaactt tatgcagcgc gtaacgtttc tctcaacagc tcccactcaa atggagcaat
    23041 tactgaagga aataaaactc caaattatgc agaagtttat gtttgttcta ctccaagtgc
    23101 tggaacaaca ggaaacaagt attgtaacgc aactgactgg tcacttgtat ttactactaa
    23161 ctcagctgta tccaatggaa cacgctttga cagttctaag tctgataaca tagcaattag
    23221 catgtttgaa atctctccaa caggaatggt ttatttaggc tttgactctc caagtgggtt
    23281 tagagtttat agaaatgcag tgggaacatc gagtgatatt acaaatactg gttggaccca
    23341 agaaggacca agtggttcca aagaaggatt tggagatgca acctacaaca gaattcagtc
    23401 ttctgtggtt gttcaagaca agaacagtgt atactatact tacatcactg taggtggata
    23461 tgacacaaca aatctttggc ataaaccgat ccaagtttat cgtcaagcag atgatggaac
    23521 tgcaagagtt gcactcggtg attttggaga cagtagctta atggcttaca tctctaagac
    23581 tgcatctagt gattctgctc gagcagcagc tgctgcaatc
//
LOCUS       DAOQOS010000086        23530 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_1030_length_23530_cov_5.896443, whole genome shotgun sequence.
ACCESSION   DAOQOS010000086 DAOQOS010000000
VERSION     DAOQOS010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 23530)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23530)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23530
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1030_length_23530_cov_5.896443"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..627)
                     /locus_tag="PLF66_17285"
     CDS             complement(<1..627)
                     /locus_tag="PLF66_17285"
                     /inference="COORDINATES: protein motif:HMM:NF013734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HMZ65848.1"
                     /translation="MKLSYNWLNDFADFKSISFEKIYEKISLSICEVDEVSEYREHLA
                     TILYAKVVEVKKHPDADKLSVCVADIGSKKLQIVTGAKVNEGEIVPLAMIGTKFPDGK
                     EIQEGKLRGVDSFGMFCSEKELGLSEESSGLMKLAADTKLGVSLRQILDSEDKIILID
                     NKSITHRPDLWSHFGFARELAAQLGIPIRFNPFESSFSFSNDSSPIVVE"
     gene            complement(918..1910)
                     /locus_tag="PLF66_17290"
     CDS             complement(918..1910)
                     /locus_tag="PLF66_17290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020767810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HMZ65849.1"
                     /translation="MAKLEHPSSALFQGEKPFPIIPACEHFAGSEKLITKALELQNKM
                     GGFFDITMDCEDGAQTGKEKEHAEMIVRIQNSDLNVHKMSGVRIHDYSNAHWRLDVDI
                     IVPGAGEKIAYITIPKPTKAAQVAEMISYIQEVAKKSNIKREIPIHVLIETHGALLEV
                     EQIAALPWMQVLDFGLMDFISGHHGAIPASAMRSPGQFEHELLRQAKATVVAAALAHG
                     VIPAHNVTLDLKNQYQTYKDASRARNDFGFLRMWSIYPTQIQAILDAMTPDYSEVQTA
                     CEILMKAQDADWGPIQHAGELHDRATYRYFWELVQKAKITGQKLPDEVTKRFFS"
     gene            complement(2118..3587)
                     /locus_tag="PLF66_17295"
     CDS             complement(2118..3587)
                     /locus_tag="PLF66_17295"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HMZ65850.1"
                     /translation="MNEPTERIDIFPWNKSFETEFEEIDTQHKKLVELLNQLARHLVY
                     QSDKITLNQIFLEITSYALYHFETEEGVWKKYLGSKDTWFLQHENEHKSFVTELLKLK
                     AEEEERPIEVVLSDILSFLTHWLAFHILESDKKLAKAAKRVKTGLPLEQAKEMAGIEM
                     EGVLSLLIDTILKMNDHLSKRTLELMREANKREMAEVKLRLASNVFENTLGAICILDS
                     QFKVMEANPSFYQSTGFIEGELIGRSLSDSKSGFREKIEMDGFIEAVNESKHWNGIIT
                     NRRKTGEMEAEWMTLSAVKNSDGSVMNYVAIFSNVSHLMEQQAFLENIAHHDTLTGLP
                     NRLLLRDRLTIAIAHSEAARTALAICYIDLDGFKPINDKYGHLAGDIVLKEIAKRLSG
                     IVRKYDTVARLGGDEFVILLGDMKSQSDCIRVLNKVLDEVQAPIQIQNGTARVTASIG
                     VTFFPEDASESDTLLHHADQAMYEAKKQGKNRYCFYYPI"
     gene            complement(3641..3913)
                     /locus_tag="PLF66_17300"
     CDS             complement(3641..3913)
                     /locus_tag="PLF66_17300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65851.1"
                     /translation="MDIFEQILKFFGIGEHQAESTIETFHNVPSIITFFQSLYPDARV
                     EKSPEDAEELGIYIQRTGSNQIFIPIRESQKSGYYLIEVPARSHFS"
     gene            4323..4844
                     /locus_tag="PLF66_17305"
     CDS             4323..4844
                     /locus_tag="PLF66_17305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
                     /protein_id="HMZ65852.1"
                     /translation="MKKLIFLIFIFHFILSITPETKDSENNYESLLEIKTSKIPKSGN
                     GVFAKENFEKGIVLGAYTGKFITEEEYNEILKRNDWHYVMWFKDCARPHLGKYVGVDG
                     KIEGNIFSRINYAPAKFQNVKFEKICEPPYALVRTLRSIQKGEELYIDYGPDYIYDFM
                     EDKEIQNFFNPPK"
     gene            complement(4828..5250)
                     /locus_tag="PLF66_17310"
     CDS             complement(4828..5250)
                     /locus_tag="PLF66_17310"
                     /inference="COORDINATES: protein motif:HMM:NF023125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3293 domain-containing protein"
                     /protein_id="HMZ65853.1"
                     /translation="MKPLNSNVLLTAYKNTLYKIFYQDKEFKIEVDKRSDFVDKLLID
                     KGVTSYIFITAANPHSLLVSEAENEKANLELENQIKQKALFYCKGVGESKDGEWKEDS
                     FFVLGISLKEGMSIAKQFSQNAFLYGRKKTKTRLIWAD"
     gene            complement(5252..6118)
                     /locus_tag="PLF66_17315"
     CDS             complement(5252..6118)
                     /locus_tag="PLF66_17315"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HMZ65854.1"
                     /translation="MSSKKISLLLVSIFLSCQILKKLNSNQDHLSQIKNSIGMELIEI
                     PTGDFAMGCRDGDPNCDEDEIPNRKTYITNPFYIGKYEVTKGEFSKFISETGFITEAE
                     KLGFKNTWKNCHGMKQTDLHPVICVSWNDAIQFTKWISSKENKKYRLPTEAEWEYSAR
                     ANDGNFNISKTENLNLSVKRYYWGFDINESFAWFNKNSNRETHPVGEKKPNAWGIYDI
                     SGNVWEWCEDSYDSNFFKRDKKIEVLNDNYKVLRGGSWFDSFLFLRISARNFALKETR
                     ESHFGFRVVMER"
     gene            6206..8191
                     /locus_tag="PLF66_17320"
     CDS             6206..8191
                     /locus_tag="PLF66_17320"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HMZ65855.1"
                     /translation="MKIIKKIIRTLLGFIFIFFSLPLLLYIPQIYDSNFYKNQITSAV
                     ETYTEYKLKLDSLDLSVYPDLKLNLKNIELKTKNKDIEILKLEEINLAVFFLPALAGG
                     KLQVDDLSLRNGFVDIPNLLSSIPEQKEESPKEEVNVKQITDIIHTKLRIKQIQIENT
                     TVYIKKLNPYIYHDPFIKKITFSFESFWRANLDLLADYGKTNLNLSISSGISPEDFSL
                     SSLVVDSNIKIANFNFLEYNQFLKDIPLQFQSSFLNLEIRTKKEKDSIVLKNEVDLFL
                     NRIGYKNDKKEDKSINGIQIKGNIYYPLLSKKIETEDLKIFVPRLLDVKVDSSLQFFK
                     KPILQLNLFSNHVYINEILDFAGAFAQTDTNKQSNEKKQTSKSEILLKLKYFAQHIKY
                     DTFDIHNFFSHIDLNNSALNYNLGLKKIADGNIQIQGEAELADYISTNADIQIENINL
                     EKFTKQFLNKKYAEGNFVTSIKLQTNNQFGEKDFLKNLYLTGFTRIEKGILLERADLL
                     YPIRFLTKIIPDSDKWNGNISRFDFMDIDYSIKNNRFKVKNLDMKGLIFNTNGSADIG
                     LNDPSNDIAVNLIVSTRIASTGLKIPLVYSKSSYVPLTVDKVWLASTYTGMVVGGPVG
                     AVVGSLLSEKAGQAFQTIHNKTKDKTEILFNNPPYTR"
     gene            8664..9194
                     /locus_tag="PLF66_17325"
     CDS             8664..9194
                     /locus_tag="PLF66_17325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65856.1"
                     /translation="MAEVKGKFITLSCSLLETKPAAKKAALEAVKNMTGKEFNELDPE
                     GWYDTKVMQSVFKAIEENSSPLLARAAIKLIGQKVYPTIEKTAGLPPTLKTPLDFIKF
                     EAEGFLLNHRGSDVKPRKIIQAEDKNVVIEANSPGYSPVLIEGVFLGILEMCMIKTGR
                     VDNKEENGNSIYYIKW"
     gene            9357..10997
                     /locus_tag="PLF66_17330"
     CDS             9357..10997
                     /locus_tag="PLF66_17330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004786396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HMZ65857.1"
                     /translation="MKWWQKTTIYQIYPRSFYDTNEDGIGDIPGIIAKLDYIQNLGFE
                     TIWISPFYKSPGKDFHYDISDYRDIDPIFGTLKDAENLIAEVHRRNMKIVFDMVLNHT
                     SDEHPWFKESRSSKTNPKRDWYIWRKGKKNSPPNNWTSMINKPGWNYDPTTDEWYFAN
                     FLSFQPDLNYRNPEVKEAMFDVMRYWLQKGVDGFRLDIFNSIYKDPEFRDNPLSFRFV
                     PTPDYQDEAFFQKKLHTLNHPDCFPLAKEVRGVIDEFSGDRFLIGEVSGNDKTLKKFL
                     GEKQDGLNLVFQFELIDFEFSAKFFKRVLIKNENTFPYPYTPTYVYGNHDQMRYIDKI
                     ENNPEKAKLLALFQFTARGVPIVYYGEEIGIRDANIPNAEGKDPIAQQNAFIPNFFVN
                     FFGIYLNRDNCRTPMQWSSAPHGGFSAPHVDPWLNVTLGFSYMNVESETKDPNSLLNA
                     YKSIIKLRNEQESFQTGNLEVVNDFDNLTDLLVFRRFSKDKNFYVFLNFGYKPLAVRV
                     KNITSISLKIGDARFDSSIVTLDAYAGIVLESDQNSIS"
     gene            complement(10987..11760)
                     /locus_tag="PLF66_17335"
     CDS             complement(10987..11760)
                     /locus_tag="PLF66_17335"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HMZ65858.1"
                     /translation="MHILITGAATGIGESIVDYWFSKFPKDKVSILDKDEAQLNATIN
                     RMKTQGIDAAGYPFDLTKIEAFESIVEKVKSERGEVDILINNAGVMIIEDFSVMSWEK
                     GHMILTLDLIAPMKLIKLLLPSMITRKSGGIINLASMAGKAPLPGCTWYGAAKAGFGH
                     ASEILAGEVKDDGIHVLTVYPGPVSTALEKGARAGYDENIVSSLMPVGDRKIIAEKIV
                     EGYLKKQLVLAYPDIYDLARIFPPLTDNFIRWFAPKTKK"
     gene            complement(11868..13394)
                     /locus_tag="PLF66_17340"
     CDS             complement(11868..13394)
                     /locus_tag="PLF66_17340"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HMZ65859.1"
                     /translation="MKQNHTRLFIIIILITTLILYITSILSISIYNNTLHNSGNWINR
                     KAQVSRSVPASASYMNTMPALFKFKLNLSQWLGYHKLIFTKEVRPKEISFQYRIDPGQ
                     YFTFIFNADSIAYRSYGIRISNNQLYPSTFLRIENDLFLEKKPLDPGFQSDTKPHRFS
                     IQIMDDSFTVMVDNQIIFTQKEKISESMFIGFKAGLTDLRSDIDDLVILQKDGSVIEE
                     SFFYWNKIPKYSVYFILFIILYFILFWILQNFYKVNPDDRLKILAYINFILVLFSIFI
                     YYAMYTRISRIYFTWSKVYEGFPVMPATDTTIRKFVDQYSVEKKGRRIWFVGSSQTWG
                     AGVYNDPDTFISILERDLQKVEKGIECINGGVSGLSSEKLLALYKEYLDKLKPDITII
                     NLSHNDAYFQVPVDQFIKNLSEFVQINQNKNITTIFSIEPNEIDYAIPNAHREAMEKL
                     AQELNIPYINMHKKMLPHNDDAFLWWDRIHFTSAGNQIFANEMYPFLAEMVLKSRLSK
                     "
     gene            13457..13639
                     /locus_tag="PLF66_17345"
     CDS             13457..13639
                     /locus_tag="PLF66_17345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65860.1"
                     /translation="MKIILIIVSVICFIWASMSFLFTAKLILDGKMLLAVIDFLVLGF
                     LPAIVGVYCFKTAIKK"
     assembly_gap    13734..13833
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(14180..15043)
                     /locus_tag="PLF66_17350"
     CDS             complement(14180..15043)
                     /locus_tag="PLF66_17350"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HMZ65861.1"
                     /translation="MNRQNLERNKFSRNDFIVKSLATGFALAVLPSCSSSSNIITTDT
                     NGLVAGEVKVSVKGGSIPAYSAYPVNGTNLPIVLVVQEIFGVHEHIKDLCRRLAKSGY
                     FAIAPELYSRQGDVSTIADWKEIISKVVSKVPDSQVMSDLDACVSYAESTKLVNTNKL
                     AITGFCWGGRITWLYSAHNAKVKAGAAWYGRLTSDKSPLQPAHPIDIAPKLKAPVLGL
                     YGGKDAGIPLAQVDQMKEALKKANSSSEILVYPEADHGFNADYRPMYNEAAAKDAWAK
                     MLSWFQKNGVA"
     gene            complement(15051..15413)
                     /locus_tag="PLF66_17355"
     CDS             complement(15051..15413)
                     /locus_tag="PLF66_17355"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HMZ65862.1"
                     /translation="MNPDLNNSDSDYGVEVSTYKNYVLISFINYHKLDLYNSRDLAEV
                     FKVQNENGSEDIVIDMSPIQYIDSSGLGTLATQGMLLSKKNKRLNLLNITHGVSHLFK
                     ASGFDRMFKLFTNKEQLP"
     gene            complement(15566..15934)
                     /locus_tag="PLF66_17360"
     CDS             complement(15566..15934)
                     /locus_tag="PLF66_17360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65863.1"
                     /translation="MDLLLLFYSVLLAFLIVAPFIYVQISEKNPISEEETRSSLESER
                     KMLLENLKDLKTDMDTKKIQTNEFSELSKQIIEDLKQIDTKLANLPKIEFKPGVCRKC
                     NFHTTIPNAKFCAMCGSSLA"
     gene            complement(15934..16485)
                     /locus_tag="PLF66_17365"
     CDS             complement(15934..16485)
                     /locus_tag="PLF66_17365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002761469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein CcmH"
                     /protein_id="HMZ65864.1"
                     /translation="MRVKILFFAIILLSFSILADSSTTTLTDLKQIEVFNEVTSRIRC
                     ICLPSLPIKSCSFNNCAISAQLKFFLENRIRKGESANEIVQKMQTGFGEEILSDPIVQ
                     KFQASGNEQMVSGLIHGMGERILADPDSTWINITLFVGIALGLGIVFYYLKDRLAKEK
                     NSKPKADSTLSEDAAKYLKEIDG"
     gene            complement(16482..18671)
                     /gene="ccsA"
                     /locus_tag="PLF66_17370"
     CDS             complement(16482..18671)
                     /gene="ccsA"
                     /locus_tag="PLF66_17370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020771111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HMZ65865.1"
                     /translation="MNNLGIICLIASFSILLFSIFQTSYGIWKKNQRAIEIGRYTLLA
                     NFFVILLAFIILMTQLARMDLNNHYVVMHSSEHLPLFYRVTSIWSGSSGSLLFWNLLV
                     SLFSFIALWETRNSKMDRIPVMHLVIAIISCFFTYLAVFYEDAQPFREFQPAAVVGRG
                     LNPLLQHWAMIIHPPILYIGYVSFAIPFAIGMSALITGNLSADWLKSIRRWSIFSWFF
                     LGTGILLGSKWAYEELGWGGYWAWDPVENASLMPWLLSSAFIHSLIIQERRGMLKFWN
                     MLLIILTYHFTLLGTWITRSGVLEGPHSFAKSTIGTPFILFIGISFVVYTAILIYRRN
                     ELKPERDLEAITSKEGSFLLNNFILVLACLVILLGVFSPLLYGKEFKAPWFNSWGVPA
                     GIFLLLLMGAAPLLAWRKGADSVFFTTLIKPLVVGVIVSLFYGVFYSKFFTQSDYSIS
                     DRLAEIYSIITVGLAGFTVAGIFQEYHRGIVARREQFQDENYFKAGFKMLLKNKRRYG
                     GYIVHFAVVLIFIGYAGNAFKQNTSIKFYYQLENTNGNEIVYRGFDKGMLGDYQIEAN
                     TLKIKPIINGDETKSPNLFNVIVSQEASFKIKQQLVSVEDMVTERRFYPQINHFTGGF
                     ETHIPTSEPSIHSSPKEDLYIQLGAIENASLSEENPDLPLLFMEYFFGARSFEEKKAI
                     FDKFPTEIVGNLEIWVNPLVKLIWFGSVLYFLSGLFILLPIGEGKKI"
     gene            complement(18668..19060)
                     /locus_tag="PLF66_17375"
     CDS             complement(18668..19060)
                     /locus_tag="PLF66_17375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016549939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017003 - protein-heme linkage [Evidence
                     IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HMZ65866.1"
                     /translation="MKVKFTILILAIAMSLMAIAFFSSKETSFILLDASELASHPDKY
                     DGDNLRVRGLVKIGSVVREGRQAKFTIELNDKEVPVNYSGKELLPDAFKEGVRVRVDG
                     KFKNGVLVGDHVEAKCASKYEAEYKNEP"
     gene            complement(19421..20929)
                     /locus_tag="PLF66_17380"
     CDS             complement(19421..20929)
                     /locus_tag="PLF66_17380"
                     /inference="COORDINATES: protein motif:HMM:NF013155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65867.1"
                     /translation="MFPFIKKSLFLSIIPIYLYQSLIYANDTEKTNAYFKKIRGNQAM
                     LTAFFNAMPKGGDLHHHFSGSVYAEKYFELAVKKNFYINLETGDSFQTIPSSLANDPN
                     VRPINSLIADKGYLPVKLKLISLWSIKDFKETQQTPDKHFFEAFRKFGPLIKGNEDVF
                     LKEIKARAIIENVQYIETMFLRPEYDRKDPKLLLFSDKYIPQLLKIQEERLETEAKER
                     SETKLSLLFDEMVKTLIVEMQYDDVAKNFADTIEVIHQKSKLPIEEEKKILIRYQNHV
                     TRTDSPADVFVQIFLSFQSDSISDLIVGTNIVGPEDNPNAMKNYWLHMEMFKYFHKKF
                     PDLKYSMHAGELSIGIVPPEDLSWHINSALFDAEANRIGHGVDIPYETDCYSLLEYMK
                     EKKIPVEINMISNEFILGIKNDFHPFLLYHKFGVPIVITSDDMGVLRTSFTEQFAILA
                     KRYRQISYIDIKGFIFNSIDYSFLPSKEKKNLRKKLEKEFIKFEDRISKIEK"
     gene            complement(21291..21536)
                     /locus_tag="PLF66_17385"
     CDS             complement(21291..21536)
                     /locus_tag="PLF66_17385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65868.1"
                     /translation="MELKEWTVYKDGMQYACSLNEKEFCFGATSGNPHGEWGSSCSIE
                     EFLLGKNQDFVQNYFPDKLKEILEYAKTLKGQSKYKR"
     gene            21722..22885
                     /locus_tag="PLF66_17390"
     CDS             21722..22885
                     /locus_tag="PLF66_17390"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HMZ65869.1"
                     /translation="MHQSFPLHHSTLLVVLAAVTFITTTLLVIAALFSDKNKSMILWA
                     KGNVFICLGFVVGALYFIPSFFYSVICYVLMGTGMGYIYAGIKKFINEDVHRSFIPLV
                     SNLAGIGPMYYLYWDDSLSGRLLVSSIYFGIINLICAWTFLKPSKTSERGASILAGSG
                     FLCMGLALLSRGIFIIKADLNNPNHFQSIETGTIFLTSILQIWTTVALIILIMNRYAN
                     QILNISITDSLTNTFNRTGLNSLGLAKLKKAELNQIPMAIILFDADHFKSINDMYGHL
                     TGDKILTYTAETAKKNLREKDLIARFGGEEFVVILEDVSSQESYLLSEKLRDAIAKNE
                     YFTGNESVHCSISIGVASTEVCNTYNLYELIDIADKALYKAKAEGRNRVVLSG"
     gene            22993..23274
                     /locus_tag="PLF66_17395"
     CDS             22993..23274
                     /locus_tag="PLF66_17395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65870.1"
                     /translation="MIQCWNYRADCYELEGLNYAASTKPNRLDRTIDSCGALALPYLV
                     NPSAKMDSWQRDTLLLNCYLVKQKRKKCDKEPNGPIPGNPYIWDKKYGK"
BASE COUNT         7813 a         4128 c         3682 g         7807 t
ORIGIN      
        1 ttccacaaca ataggagaag aatcatttga aaaagaaaaa ctagattcaa atggattgaa
       61 acgaattgga attccaagct gtgctgcaag ttctcttgca aatccaaaat gactccaaag
      121 atcgggtcta tgtgtgatgg atttattatc aattaaaatg attttatctt cgctgtctaa
      181 aatttgacga agactgactc ctagttttgt atcagcggct aatttcatga gtccggaaga
      241 ttcttcactg agtcctaatt ctttttcaga acaaaacatt ccaaaactat ctacacctcg
      301 aagctttcct tcttggattt cttttccatc cggaaacttg gttccaatca tagcgagtgg
      361 aacaatctct ccttcattta cctttgcacc tgttactatt tgtagttttt tagagccgat
      421 atccgcaaca catacactta gtttatctgc atcgggatgt ttttttacct ctaccacttt
      481 cgcatataga atggtagcaa gatgttctct gtattcagat acttcatcta cttcacaaat
      541 agaaagactg atcttttcat agattttttc aaacgagata gatttaaaat cagcaaaatc
      601 attgagccag ttataagata gtttcatagt ttttgacctc tccccgactt tctattctct
      661 gaaaaatttt ctagaacaaa gcccctctcc taaaggagaa aggcagactg gaagatgggg
      721 aaaggttctt gtccttttag tatttcaata gtagcgttag cgattgaggc ggtgtcaatt
      781 atttgcggat gcaaataatt gacagtagcc gaaagcgcgg cggcgttttt tcgtaaatgt
      841 agggaacgca tatatgcgtt ccctacgacg ataataatgt cgccgcaacg ccccaatgga
      901 ctgaataaat tcaatcctta agagaagaat ctctttgtta cttcgtctgg tagtttttgt
      961 ccggtgattt ttgctttttg aacgagctcc cagaaataac gataggttgc acggtcatgc
     1021 aattcgccag catgttgaat tggtccccaa tctgcatctt gtgctttcat tagaatctca
     1081 caagctgttt gcacttctga ataatcagga gtcattgcat ctaggatcgc ttggatttga
     1141 gttggataaa tagaccacat acgtaagaaa ccaaaatcat ttcttgcacg gctagcatct
     1201 ttataagttt ggtattggtt tttgagatct aatgtaacgt tatgtgctgg aatcactcca
     1261 tgtgcaagag ctgcagctac tacagtcgct ttcgcttgac gtagtaattc atgctcgaac
     1321 tgaccagggc ttctcattgc agaagcagga atcgcaccat ggtgaccaga aataaaatcc
     1381 atgagtccaa aatcgagaac ttgcatccaa ggaagagcag caatttgttc tacttctaga
     1441 agcgctccat gcgtttcaat taaaacgtga attggaattt ctcttttgat atttgatttc
     1501 tttgccactt cttggatata agaaatcatt tcagccactt gagctgcttt tgttggctta
     1561 ggaattgtga tataagcaat tttttctcca gcacctggaa cgattatatc tacatctagt
     1621 ctccagtgag catttgaata gtcgtggatt cgcacaccac tcattttatg aacattcaaa
     1681 tcagaatttt gaatgcgaac aatcatctct gcgtgttctt tctcttttcc tgtttgtgca
     1741 ccatcttcgc agtccatggt tatatcaaag aatccgccca tcttgttttg gagctcgagt
     1801 gcttttgtaa tcagcttttc ggatccagcg aaatgttcac aagctggaat aattgggaat
     1861 ggtttttccc cttgaaataa tgctgaactt ggatgttcta attttgccat aaatctacct
     1921 ttttcatgcc ctaagcgtgg gcgtattata tatactaata ctatggctac aaaagcgact
     1981 cttacgtaaa ttcaattttt aatgaatcga gagtcaaatt tgttgggaaa ttcttgaata
     2041 aagccacaaa agactgtgat gagccaaagg atagatattt ccgaatcaaa aaatcaccta
     2101 tcggagtcta tcgtccttta aataggataa taaaaacaat atcgattttt gccttgtttt
     2161 ttggcttcat acatagcttg gtctgcatga tgaagtaaag tatctgattc acttgcatcc
     2221 tcagggaaaa aagtaacccc gatactagca gttacacgag ccgttccatt ttggatttga
     2281 attggagctt gcacttcatc caagacttta tttaaaactc ggatacaatc agattgactc
     2341 ttcatatctc caagtaagat tacaaattca tcaccgccta atcttgccac tgtatcatac
     2401 ttacgaacaa taccggaaag tcgctttgca atttctttca agactatatc gccagctaaa
     2461 tgaccatatt tatcattgat tggtttgaat ccatctaggt caatataaca aatcgcaaga
     2521 gctgttctag ctgcttctga gtgagcaatc gcaattgtga gtcgatctct taaaaggagg
     2581 cgatttggta aacctgtcag tgtatcatga tgagcaatgt tttctaaaaa tgcttgttgc
     2641 tccatcaaat gacttacatt tgaaaatata gccacatagt tcataacgct tccatcagaa
     2701 tttttaaccg cagacagtgt catccattct gcttccattt cccctgtctt tcttcgattt
     2761 gtgataatcc cattccaatg tttagattca ttcacagctt caataaaccc atccatttcg
     2821 atcttctcac ggaaaccaga ttttgaatca ctcaaagaac gaccaatgag ttccccttct
     2881 ataaatccag tgctttgata aaaggaggga tttgcctcca ttactttaaa ctgtgaatct
     2941 aaaatacaaa tagctcctaa cgtattttca aatacattgg aagcaagacg aagttttact
     3001 tctgccattt ctcttttatt ggcttctctc atgagttcta aagttctttt ggagagatga
     3061 tcattcattt ttaaaattgt atcaatgaga agagacaaga ctccttccat ttcaatgcct
     3121 gccatctcct ttgcttgttc gagcggcaaa ccagtcttaa ccctttttgc tgcttttgct
     3181 aacttcttat cgctttctaa aatatgaaat gctagccaat gagtcaaaaa cgatagaata
     3241 tcagataaaa caacctcaat gggtctttcc tcttcttctg cttttagttt taataattct
     3301 gttacgaaac ttttgtgctc attctcatgc tgtagaaacc aggtatcttt actacctagg
     3361 tattttttcc aaactccttc ttcggtctca aaatgataaa gagcataaga tgtaatttca
     3421 aggaagattt ggtttagagt gattttgtca gattgataaa ctagatgtct agcgagttgg
     3481 ttcaaaagtt ctaccaactt tttatgttgt gtatctatct cctcgaattc tgtttcaaaa
     3541 cttttattcc aagggaaaat atctatacgt tcagtaggtt cgttcattgt attatatacc
     3601 aaactaaatt caaataccca tcaaaaattc atttgatgaa tcaagaaaaa tgagaccttg
     3661 caggaacttc gatcaaataa tatccacttt tttggctttc tcgaattgga ataaagattt
     3721 gattagatcc agttctttga atataaattc caagttcttc agcatcttct ggagattttt
     3781 caacccttgc atctggataa agactttgaa aaaaagtaat aatagaaggg acattgtgaa
     3841 aggtttctat cgttgactca gcttggtgtt ccccaatgcc aaagaatttt aaaatttgtt
     3901 caaatatatc catatcaccc tctttgtttc ttccagtttt tacaataatt ctattttatc
     3961 aatctgaaaa aggcagcttc agagtaacta gatgagttct gtatccatca ccaaaaacag
     4021 gattcatttt gtttaaaaaa aaattggctg atttcaaatc aaatattttg caaaaacatt
     4081 tggagcttac aaactttacc cttttctaaa tacgagatct atttcttctt taatttgaaa
     4141 aactttcaag cagcaaacat cctagataaa aaaatcacaa attaattttg aatcattcta
     4201 attgattttt ccaaaataaa agtttactaa ataatacttc aaaaaagcaa ataacgttcg
     4261 tttgcactag aaaatatttc ttttcaaaaa tacattttta tattctattg aattcaactg
     4321 ctatgaagaa acttattttt ttgattttta tatttcattt cattctatct ataacgccag
     4381 aaacaaaaga ttctgaaaat aattacgaat ctcttttaga aattaaaacc tctaaaattc
     4441 cgaaatcagg aaatggagtt ttcgctaaag agaattttga aaagggaatt gtattgggtg
     4501 cttatacagg aaaatttatc acagaagaag agtataatga aattttaaaa cgaaatgatt
     4561 ggcattatgt aatgtggttc aaagattgtg ctagacccca ccttggtaag tatgtcggtg
     4621 tagatgggaa aatcgaagga aatatatttt cgagaatcaa ctacgctccc gcaaagtttc
     4681 aaaatgttaa attcgagaaa atatgcgagc caccctacgc actggttaga acgttacgct
     4741 ctattcaaaa aggagaggaa ctttatatcg attacggtcc tgattatatt tatgatttta
     4801 tggaagataa agaaatacag aatttcttta atccgcccaa ataagacgag ttttcgtttt
     4861 ttttctgcca tataaaaaag cattctggga aaattgtttt gcgatagaca ttccttcttt
     4921 tagagaaatt ccaagcacaa aaaatgaatc ttctttccat tctccatctt tagattcccc
     4981 aactccttta caataaaaca aggctttttg tttaatttga ttctctaatt ctagatttgc
     5041 tttttcattt tctgcctcag atacaagtag agaatgcggg tttgcagctg taataaaaat
     5101 ataagacgtt acacccttat cgattagaag tttatccacg aagtcagacc ttttatctac
     5161 ttcgatttta aactctttat cttgatagaa tatcttatat agtgtatttt tataggctgt
     5221 taataataca tttgaattta gcggtttcaa tttacctttc cattacaact ctaaatccaa
     5281 aatgactttc tcttgtttct tttaaagcaa aatttctagc agaaattcgg agaaataaaa
     5341 aggagtcaaa ccaacttcct ccccgcaaaa ccttataatt atcatttaaa acttctattt
     5401 ttttatctct tttaaaaaaa ttcgaatcat agctatcctc acaccattcc caaacatttc
     5461 ctgaaatatc atagattccc caagcattcg gctttttctc tccaaccgga tgagtttctc
     5521 gatttgaatt tttattaaac caagcaaagg actcattgat atcgaagccc caataatatc
     5581 tttttacaga tagatttaaa ttttcagttt ttgaaatatt aaaatttcca tcatttgccc
     5641 tagcagaata ttcccactcc gcttctgttg gtaggcgata ttttttattt tctttgctag
     5701 aaatccattt tgtaaattgt atcgcatcat tccaactaac gcatataaca ggatgtaaat
     5761 ctgtttgctt cattccatgg caattcttcc atgtattttt aaatcctagt ttctcagctt
     5821 ctgttataaa acctgtttca gaaataaatt tagaaaattc tcccttcgta acttcatatt
     5881 ttccaatata aaaaggattc gtgatataag tcttacgatt tggaatttca tcctcatcac
     5941 aattagggtc accatctcta caacccatgg caaagtcacc agtaggaatc tcgattaact
     6001 ccataccaat cgagtttttt atctgggaca aatgatcttg attcgaattt aattttttca
     6061 aaatctgaca ggataaaaat atactcacta agagtaatga tatttttttt gaactcaatt
     6121 taatttcatc cttgctaaag aatttgacat attataattg cacagagcat ccctgcaagg
     6181 cattttggaa atggtatgag ttgaaatgaa aattataaaa aaaattatac gcaccctgct
     6241 aggattcatt tttatatttt tctctctacc acttctactc tatattccac aaatctatga
     6301 ttctaacttt tacaaaaacc aaattacttc cgcagttgaa acctatacgg aatacaaatt
     6361 aaaattagat tcacttgatt tatctgtcta tccagaccta aaactaaact taaaaaatat
     6421 tgaattaaaa actaaaaaca aagatattga gatcttaaaa ctagaagaaa taaatttagc
     6481 agtattcttt ctacctgcac tcgcgggagg caaattacaa gtagatgatc ttagtctcag
     6541 gaatggattt gtggatattc caaatttact aagctccatt ccagaacaaa aagaagaatc
     6601 tccaaaggaa gaagtgaatg taaagcagat aacagatatt atccacacaa aactaagaat
     6661 aaaacaaatt caaattgaaa acacaaccgt ttatataaaa aaattaaatc cttatatcta
     6721 ccatgatcca tttataaaaa aaataacatt tagttttgaa tccttctgga gagctaatct
     6781 agatttactc gcggattatg gaaaaacaaa cctgaattta tctatttcaa gtggaatctc
     6841 gcctgaagac ttttctctat cctctcttgt agtcgattcc aatattaaaa tagcaaactt
     6901 caatttttta gagtataacc aatttctaaa agatattccc cttcaatttc aatctagttt
     6961 tttaaattta gaaattagaa caaaaaaaga gaaagattcc attgtgttaa aaaatgaagt
     7021 agatttattt ttgaatagaa ttggatataa aaatgataaa aaagaagata aatctatcaa
     7081 tggaattcaa attaagggta atatctatta tccgctttta agtaagaaaa tagaaaccga
     7141 agatctaaag atatttgttc cccgcctact agatgtaaaa gttgattcta gtttacagtt
     7201 ttttaaaaaa ccaattcttc aattgaatct tttctcaaac catgtctata taaatgaaat
     7261 attagatttt gcaggtgcat tcgctcaaac agatacaaat aaacaatcca atgagaaaaa
     7321 acaaactagt aagtctgaaa tactcttaaa attaaaatat ttcgcacagc atataaaata
     7381 tgatacattc gatattcata attttttttc acatatcgat ctaaataatt ctgctttaaa
     7441 ttacaattta ggattaaaaa aaattgcaga tggaaatata caaatacaag gagaagcaga
     7501 attagctgat tacatatcta ctaacgcaga tattcaaata gaaaatataa accttgaaaa
     7561 atttacaaaa cagtttttaa acaaaaagta tgcagaagga aattttgtaa cttctatcaa
     7621 attacaaaca aacaatcaat ttggagaaaa agacttctta aaaaatctat atcttactgg
     7681 atttactcgt atcgaaaaag gtattttatt ggaaagagca gatcttttat atccaattcg
     7741 gtttttaact aaaatcattc cagatagtga taaatggaat ggaaatattt ctagatttga
     7801 ttttatggat atagattatt caatcaaaaa caatcgcttc aaagtaaaaa atctagacat
     7861 gaaaggattg atttttaata caaatgggtc tgccgatatt ggtttaaatg atccctctaa
     7921 tgatatagcg gttaatttaa tagtatccac tcgaattgca agcacaggtc taaaaatacc
     7981 tcttgtatat tctaaatcca gctatgtccc actcactgtg gataaagtat ggttagcctc
     8041 tacatatact ggtatggttg ttggaggtcc tgtaggtgca gtggttggat ccttactatc
     8101 agagaaagca ggacaggcat ttcaaacaat tcacaataaa acaaaggaca aaacagaaat
     8161 tttatttaac aacccacctt atacaaggtg aaatatttct ttgagacttt ttttattagc
     8221 ctaattaaaa attgcaaaag tttttatcaa aggaataaag aaagatgaga tatcttacct
     8281 tttaatttaa cattcaccaa atttagcatt cccttttatt atcaaaagaa tcttttttaa
     8341 tcccacacta gattcaagat gtttcctaaa taccattgaa tttaagaata tttactctcg
     8401 ttttgaggca aatatgtctt atcctcattt tactaaagaa atgaaaatga tttctctgtt
     8461 tgccattaac cgcatatttt tttaaaatat ctctcatgaa ttctttaata cattacagaa
     8521 tcaatattta taatttttct tttttgattg agtcgattgt attaagaaaa taaaaatctc
     8581 taaataactg aattctataa tcaatcgtaa aattatcaga agaatgttaa ctaaactcag
     8641 gaaaataaat aaagaggaaa attatggcag aagtaaaagg aaaatttata acactatcgt
     8701 gtagtctact cgaaacaaaa ccagcggcaa aaaaggcagc attagaagct gtaaaaaata
     8761 tgactggtaa agaattcaat gaactagatc ccgaaggctg gtatgataca aaagttatgc
     8821 aatcagtatt caaagcgata gaagaaaact catcacctct tctagcaaga gcggcaatta
     8881 aattaatagg acagaaagta tacccaacga tagaaaaaac agcaggtctt ccacctactt
     8941 taaaaacacc actcgatttt ataaaatttg aagcagaagg attcttactc aatcacagag
     9001 gatcggatgt caaacctaga aaaataattc aggctgagga taaaaacgtt gtcatcgaag
     9061 cgaactcgcc tggctactcc cctgttttga ttgaaggtgt ttttttagga attttagaaa
     9121 tgtgtatgat aaaaacaggt cgagttgata acaaagagga aaatggaaac tcaatttatt
     9181 acattaaatg gtaatcattt tgggaagtat tttatttaaa aattaaatag aatcagaatc
     9241 tgaaagcgga taataaccgt tatccgtttt aattcaaaac cagacatcaa aaaacttttt
     9301 catatgtgtt tacaatctca ggctttctgt aagccttgca agagaaggag ttttttatga
     9361 agtggtggca aaaaactaca atctaccaaa tctatccaag atctttttat gatacaaatg
     9421 aagatggaat tggggatatt ccaggcataa tcgcaaaatt agactacatt caaaaccttg
     9481 gatttgaaac gatctggatt tctccctttt acaaaagtcc tggtaaagac tttcactacg
     9541 atatcagcga ttacagagat attgatccaa tttttggaac tcttaaagat gcagaaaact
     9601 taattgcaga agtgcataga agaaatatga aaattgtttt cgatatggtt ttaaatcata
     9661 cttcagacga acacccttgg tttaaagaat ctagatcgag taaaaccaat ccaaaaagag
     9721 actggtatat ttggcgaaag gggaaaaaga attctccacc taataattgg acttcgatga
     9781 tcaataaacc tggttggaat tatgacccaa ctactgatga gtggtatttc gcaaattttc
     9841 tttcctttca gcctgattta aattatcgta atccagaagt caaagaggct atgtttgacg
     9901 ttatgcgtta ttggttacaa aaaggagtag atggttttag attagatatt tttaacagta
     9961 tttataaaga tccagagttt agagataatc cattgtcttt tcgatttgtg cctactcctg
    10021 actatcaaga tgaagctttt tttcaaaaaa aactgcatac cctaaatcat ccagattgct
    10081 ttcctctcgc gaaagaagtg agaggagtaa tcgatgaatt tagtggagat cgttttctga
    10141 ttggagaggt aagtggaaat gataaaactt taaaaaaatt tttaggggaa aagcaagatg
    10201 gactcaatct agtctttcaa tttgagctaa ttgattttga attctctgca aagttcttta
    10261 aaagagtttt aattaaaaac gaaaacacat ttccctaccc ttatactccg acctatgttt
    10321 atggaaacca tgatcaaatg cgttatatag ataaaattga aaacaatcca gaaaaggcta
    10381 aactacttgc attgtttcaa tttactgcca gaggtgtgcc tatcgtatat tatggagaag
    10441 aaattggaat tagagatgcg aatattccga atgcggaagg taaagatccg atcgcacaac
    10501 aaaatgcatt tattccaaac ttttttgtaa acttctttgg aatctattta aatcgagaca
    10561 attgtagaac accgatgcaa tggagttctg caccgcatgg aggtttttct gctccccatg
    10621 tggatccatg gctaaatgta actcttggct tttcttatat gaatgtagaa tccgaaacaa
    10681 aagatccaaa ttctcttttg aatgcttaca aatctatcat aaaacttaga aatgaacagg
    10741 aatctttcca aactggtaat ttagaagtag taaatgattt tgataattta acggatcttc
    10801 tagtatttag aaggttcagt aaagataaaa acttttatgt atttttaaat tttgggtata
    10861 aaccgttagc tgttagagta aaaaatatta caagcatctc tttaaaaata ggagatgctc
    10921 gctttgattc aagtattgta actctagatg cctatgcagg tattgttcta gaatcggatc
    10981 aaaattctat ttcttagtct taggagcaaa ccaacgaata aaattatctg tgagtggtgg
    11041 aaagattcta gcaagatcat agatatcagg ataagcaaga acaagttgtt tctttaaata
    11101 tccttctact attttttcag ctataatctt tctgtctccg actggcatta gactggaaac
    11161 tatattttca tcatagccag cacgagcacc tttttccaaa gcagtgctca ctggacctgg
    11221 atatacggtt aatacgtgta tgccgtcgtc ttttacttca cctgctaaaa tttcagaagc
    11281 gtgcccgaat ccagcctttg ctgctccata ccaagtgcaa ccgggtaatg gtgctttgcc
    11341 cgccatactg gctagattga taatcccacc actttttctt gtaatcatag aaggaagaag
    11401 taatttaatt aacttcattg gagcaattaa atctaatgta agaatcatat gacccttctc
    11461 ccaactcatt acactaaagt cttctatgat cattacacct gcattgttga ttaagatatc
    11521 aacttctccc cgttctgatt taactttttc aactatcgat tcaaaagctt caattttagt
    11581 taaatcaaat ggataccctg ccgcatcaat cccttgtgtt ttcattcgat tgatggttgc
    11641 atttaattgt gcttcatctt tatctaagat tgaaacttta tcttttggaa atttcgaaaa
    11701 ccaatagtct acgatacttt cccctattcc tgtcgccgcg cctgttatta atatatgcat
    11761 agtgtaaatt tctaaagagt ctcttttttt gtccaatcag aaatgaagag caaaaactta
    11821 ctttatttct ataatctaga aaataaaaat ctactcaagt ctagatacta ttttgagagt
    11881 cgactcttta aaaccatttc tgctaaaaag ggatacattt catttgcaaa gatttgattt
    11941 cctgcacttg taaaatgaat tctatcccac cataaaaatg catcatcatt gtgagggagc
    12001 atttttttat gcatgttaat atatgggata tttaattcct gtgcaagctt ttccatcgct
    12061 tctctatgtg cgttaggtat cgcataatca atttcatttg gttctataga gaaaatagtg
    12121 gttatatttt tattctgatt gatttgaaca aactcactta aattttttat aaactggtct
    12181 actggaactt gaaagtaggc gtcgttatgc gatagattta ttattgttat atctggtttt
    12241 aacttatcta gatattcttt atatagtgct agcaatttct cgcttgaaag accggaaact
    12301 cctccattga tacattcgat tcctttttct actttttgta agtctctttc taaaatggaa
    12361 ataaatgtat ctggatcatt gtaaactcct gctccccatg tttgggaaga tcccacaaac
    12421 caaattcgtc ttcctttttt ttctactgag tattgatcta caaattttcg aatggttgta
    12481 tcagtagccg gcataacagg aaagccttca tatactttgg accaagtaaa atagattctg
    12541 gatatacgag tatacatagc ataataaata aaaatggaaa ataaaactaa aataaaattt
    12601 atataagcaa gaattttgag tctatcatct ggatttactt tatagaagtt ttgaaggatc
    12661 caaaaaagaa taaaatacaa aattataaat agaataaaat aaacggaata tttaggtatt
    12721 ttattccaat agaaaaaaga ttcttcaatc acacttccat ctttttgtag aataaccaaa
    12781 tcatcaatat cagatcgtaa atctgtaaga cctgctttaa atccaataaa catggattca
    12841 gaaatttttt ctttttgagt aaatatgatt tgattgtcta ccattacagt aaaggaatca
    12901 tccattatct gaatcgagaa tctatgtggt tttgtatcgg attgaaagcc tggatcaagt
    12961 ggcttttttt caagaaaaag atcattttct attcgtaaaa aagtggatgg atagagttga
    13021 ttattactga ttcgaattcc atatgaacga taggcgattg aatctgcatt aaatatgaat
    13081 gtaaaatatt gacccggatc aattctatat tgaaatgaaa tttcttttgg tctaacttct
    13141 tttgtaaaaa ttagtttatg ataacctaac cactgagata aattcaattt gaatttaaat
    13201 aaagctggca tagtattcat gtaagaagcg gatgctggaa cagatctact cacttgtgct
    13261 tttcgattga tccagtttcc tgagttatga agtgtattat tgtaaatact gatagataat
    13321 atcgatgtga tgtacaaaat aagagttgta atcaatataa ttattataaa aagtcgagta
    13381 tgattctgtt tcatttcttt accaatttat aaagactaaa gaattgaaaa gaatttatta
    13441 gtgttatagg actgtcatga aaattatttt aattattgtg agcgttattt gttttatttg
    13501 ggcaagtatg tcttttttat ttaccgcaaa actcattcta gatggaaaaa tgctacttgc
    13561 tgtgattgat tttttagtat taggattttt acccgctata gttggagtct attgtttcaa
    13621 aactgcaatt aaaaaatgag atccattttt aaatactcac cttatttcca tagctcagtc
    13681 agatatttga taggcaatcg tcctaaacac catttcattc cgttaagaaa aaannnnnnn
    13741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngcctccc aagttcgtaa catattgtag
    13861 aaaaaagttc cctatctgaa tgagattggt cgatcgcata aaacaaggca tggcaacgga
    13921 aatcaattca acttatttta ataaaattca tgagaagtat ttagagccta cattctaaac
    13981 aaaatatttt tatctaggtt ttaaagatag aaagcttagt atgaaagagg aatgaactaa
    14041 gcgctttgtt tttctactta ggttggtaaa agaataaaga tttgtgctaa aaactagata
    14101 acaccgatca aaaatacaat tttttcttta aaccaatcgg ttaaaatcat tctgtgatcg
    14161 gtgtcctcgg tcgtaaaaat tacgctacac cgtttttttg aaaccagctt agcatttttg
    14221 cccatgcatc ttttgctgcg gcttcgttgt acattggacg atagtcggca ttaaaaccat
    14281 gatcggcttc tggatataca aggatctctg aagaagagtt agctttcttt aatgcttctt
    14341 tcatctgatc tacttgcgcg agtggaattc cagcatcctt tccgccataa agtccaagaa
    14401 caggagcttt taatttaggt gcaatgtcga ttggatgagc tggttgtaag ggagatttat
    14461 cggaagtaag tcttccatac caagccgctc cagcttttac ttttgcgtta tgtgctgagt
    14521 agagccaagt gatacgacca ccccaacaaa atccagtaat ggcaagcttg tttgtattga
    14581 ctagctttgt agattctgcg taagaaacac atgcatctaa gtctgacatc acttgtgagt
    14641 caggaacttt agaaactact ttagaaatga tttctttcca gtctgcgata gtagaaacat
    14701 ctccttgtct agaatagagt tcaggggcta ttgcaaagta gccgctctta gcaagtcttc
    14761 tacataaatc tttgatatgc tcatgcactc caaagatttc ctggacaaca agaacgatcg
    14821 ggagatttgt tccatttact ggataagcgc tgtaagcagg aatgcttccg cctttaacag
    14881 atactttgac ctcaccagcc acaagtccat ttgtatcggt tgtgatgata ttactagaag
    14941 aagaacaact tggtagaacc gcgagtgcaa atccagtagc aagagactta actataaaat
    15001 catttcttga aaatttgttt ctttctaaat tttgtcggtt catatcatct ctaaggtaat
    15061 tgttctttat ttgtaaatag tttaaacatt ctatcaaagc cacttgcttt aaatagatga
    15121 gatactccat gtgtaatgtt tagtaaattt aaacgtttat tctttttaga caaaagcatt
    15181 ccctgtgtgg ctaatgtgcc taatccgcta gaatcaatat attgaattgg agacatatca
    15241 ataactatat cttctgatcc attctcattt tgcactttaa atacttctgc aagatccctt
    15301 gaattataaa gatccagttt atgatagttt ataaaagaaa taagaacata gtttttataa
    15361 gtgctaacct ccactccata atcagaatct gaattgttta agtctggatt catctagcct
    15421 ccttgcctga atcgatgcgg tgctaatatt acaaatataa gcaatcattg caaacttttt
    15481 ttaaagtaga atgaaagaaa tcctatttct cataggaatg ttcttaattt tgagaaatct
    15541 ttatatgaaa ccaaagttct cagtttcaag ctagagaact gccacacatc gcacaaaact
    15601 ttgcatttgg aatcgttgta tgaaaattgc attttctgca aacacccggc ttgaattcaa
    15661 ttttgggaag gtttgcaagt tttgtatcaa tttgctttaa atcctctatg atttgtttag
    15721 acagttctga aaattcattc gtttgaattt tctttgtatc catatccgtc tttaaatctt
    15781 ttaagttttc gagtaacatt ttcctttctg actccagcga agaccgagtc tcttcctctg
    15841 aaattggatt cttttctgaa atctgaacat agataaaagg agccactatt aaaaaagcaa
    15901 gtaatacaga ataaaataaa agtaatagat ccatcagccg tcgatctcct ttagatactt
    15961 tgcagcatct tctgatagag tagaatctgc tttgggctta gaattttttt ctttagcaag
    16021 tcgatctttt aaataataaa aaacaattcc gagtccaagt gcaattccta caaagagtgt
    16081 aatattaatc caagttgaat ctggatccgc gagaattcgt tcccccattc catgaatcaa
    16141 tcctgaaacc atttgttcat ttcccgaagc ttgaaatttt tgaacgatag gatcagataa
    16201 tatctcttct ccaaatcctg tctgcatttt ttgcacaatt tcatttgcag actcaccttt
    16261 tcgaattcgg ttttctagaa agaatttaag ttgggctgag atagcacaat tattaaagga
    16321 acaactttta ataggaagag aagggaggca aatgcatctt atccgcgaag ttacttcatt
    16381 gaatacttca atctgcttta aatctgtcaa tgtggtggtg ctcgaatctg ctaaaatgga
    16441 aaacgataga agaatgattg caaagaataa aatttttact ctcatatctt ttttccttct
    16501 ccaattggta acagaataaa caaaccggat aagaagtata gaacagatcc aaaccaaatc
    16561 aatttcacta gcgggtttac ccatatttcc aaatttccca caatctcagt tggaaattta
    16621 tcaaagattg cttttttttc ttcaaagctt ctggctccga aaaaatattc cataaatagt
    16681 aggggcaaat ctggattttc ctcagataaa gaagcatttt caatcgcacc gagttgaatg
    16741 tataaatcct ctttgggact agaatgaata gaaggctcgc tcgttggaat gtgtgtttca
    16801 aatccaccgg taaaatgatt gatttgagga taaaatcttc tctctgtcac catgtcttca
    16861 acagagacta attgttgttt tattttaaaa cttgcttctt gcgatacgat cacattaaat
    16921 aagttaggag attttgtttc atccccattt ataatcggtt ttatctttag agtatttgct
    16981 tctatttgat aatcacctaa cattccttta tcaaatcctc tatagacgat ttcatttcca
    17041 ttcgtatttt ctaactgata gtaaaattta atagatgtat tttgtttgaa ggcatttcct
    17101 gcatacccga taaaaataag cactactgca aagtgaacaa tgtatccacc atacctacgc
    17161 ttatttttga ggagcatttt aaagcctgct ttgaaataat tctcatcttg aaattgttct
    17221 cttctcgcaa caattcctct atgatattcc tgaaaaattc ccgcgacagt aaaacctgct
    17281 aagccaactg tgataataga ataaatctcg gctaacctat cacttatcga ataatcactt
    17341 tgcgtaaaaa atttagaata aaaaactcca tagaaaagtg atactatgac tcctacaact
    17401 aacggtttta ttagagtagt aaagaaaact gaatctgcac cttttctcca agctaaaaga
    17461 ggagcggctc ccatgagtaa taaaagaaaa attccagccg gaacacccca tgaattgaac
    17521 caaggtgctt tgaattcttt tccgtataac aaaggagaaa atactccaag taaaatcaca
    17581 agacaagcca aaactaaaat aaaattattt aatagaaaac ttccttcttt tgacgtaata
    17641 gcctctaagt ctctttccgg ttttaattca tttctgcgat aaattaagat agccgtatag
    17701 actacaaaac taattccaat aaataaaata aaaggagtcc caatggttga ctttgcgaaa
    17761 ctatgcggtc cctctaagac tccacttcta gtaatccatg tgccaagtaa tgtaaaatga
    17821 tacgttagta taatcagaag catgttccaa aacttcaaca tccctcttct ttcttgaata
    17881 atcagagaat gaataaaggc actcgataga agccaaggca tgaggcttgc attctcaaca
    17941 ggatcccaag cccaatatcc accccaaccc aattcctcat aagcccactt cgatcctagt
    18001 aaaattccag ttcctaaaaa aaaccaactg aatatagacc atctacgaat cgactttaac
    18061 caatctgcag acagatttcc cgtaataagc gcagacattc caattgcaaa aggaatagca
    18121 aaactaacgt atccaatata taaaataggt gggtgaataa tcatcgccca gtgttgcaaa
    18181 agtggattga gtcctctacc tacaacagca gccggttgaa actctctaaa tggttgcgcg
    18241 tcttcataaa aaaccgctag ataggtgaag aaacaagaaa taattgcaat cacaagatgc
    18301 atgactggaa tacgatccat tttagaattg cgtgtttccc aaagagcaat aaaggaaaat
    18361 aaacttacaa gtaaattcca gaataaaaga gaaccggaag atccagacca tatcgaagtt
    18421 actcgataga ataaaggcaa atgctcactc gaatgcataa ctacataatg gttatttaaa
    18481 tccattcgag ctagttgagt catgagtatg ataaatgcta atagtattac aaaaaaatta
    18541 gctaaaagag tataacgtcc aatttcgatt gctctttggt tttttttcca gattccataa
    18601 gaggtctgaa aaatactaaa aagcagaatg gagaaagaag cgataagaca aatgattcct
    18661 agattattca tggttcgttt ttatactctg cttcatattt ggaagcacat tttgcttcta
    18721 cgtgatcccc aacgagaact ccatttttga atttcccatc gactcgaacc cgcactcctt
    18781 ctttaaaagc atcgggtaac aactctttgc cgctataatt taccggaact tctttatcat
    18841 tgagttctat tgtaaacttg gcttgccttc cctctctcac aacagagcca atttttacaa
    18901 gtccacgcac tcgtaaattg tccccgtcgt atttatcggg gtgacttgct aattcacttg
    18961 catcgagtaa tataaacgat gtttcttttg aggaaaaaaa tgcaatcgcc attaaagaca
    19021 ttgcaatcgc taaaattaaa attgtaaatt ttactttcat agtctatctg tttagacttt
    19081 ttcaacctag gtaaaagaaa agcaatttat taatgaagga tgaataagcc atttatttaa
    19141 tccaaaacac gaaaagtatc atcctcaaaa ggagggaaac tagaacgttg atacattgtc
    19201 cgatttttcc tttgaattct tcgtgctctt tttcgcaaga aatgagtgat tcagcatcaa
    19261 ccgattgaat tgtatcctga ctcaaaatta aatcagaaac gatgcttacc ttttctagac
    19321 aagtttactc taaacggttc tcagtttact taaatcctct ttaagctcat cagaaaccac
    19381 tgtccaaaga attagggttt gaattctttc gattcataga ttacttttca atcttagaaa
    19441 ttctgtcttc aaatttaata aattcttttt ctaatttctt tcttagattc tttttttctt
    19501 tagagggaag aaaactataa tcaatactat taaaaataaa tccttttata tcaatatacg
    19561 aaatttgtcg atagcgtttg gcgagtatcg caaattgttc tgtaaaacta gttctaagca
    19621 cccccatatc atcacttgta atcacaatag gcactccaaa cttatgataa agtaaaaagg
    19681 gatgaaaatc atttttgatt cctaggataa actcattgct aatcatattg atctcaactg
    19741 gaatcttttt ttctttcata tactctaaga gggaataaca atcggtttca tagggtatat
    19801 cgaccccgtg cccaatacga tttgcctctg catcaaaaag tgccgagttt atatgccaag
    19861 ataaatcctc tggtggaaca attccaattg ataattctcc tgcatgcatt gaatatttaa
    19921 gatcaggaaa ttttttatga aaatacttaa acatctccat atggagccaa taatttttca
    19981 ttgcattggg attatcttcc ggtcctacaa tgtttgtccc aacaattaaa tcagaaatcg
    20041 aatcggattg aaaagagaga aaaatctgaa caaatacatc cgcaggacta tctgttcgag
    20101 taacgtggtt ttgatatcga atcagtattt ttttttcttc ttcaattgga agctttgatt
    20161 tttggtgaat gacttcaatt gtatctgcaa agtttttggc aacatcatca tattgcattt
    20221 caacgatgag tgtctttacc atttcatcaa ataatagaga aagttttgtc tcagagcgtt
    20281 cttttgcctc tgtctccaaa cgttcttctt gaatttttaa caattgtgga atatatttat
    20341 cgctaaataa taaaagcttt gggtcttttc tatcatactc aggacgcaag aacatagttt
    20401 caatgtattg aacattttct ataattgctc ttgctttaat ttcttttagg aaaacatctt
    20461 cgtttccttt gatgagtgga ccaaactttc gaaatgcttc aaaaaaatgc ttatcaggtg
    20521 tctgttgcgt ttctttaaag tctttaattg accataaaga aatcaatttg agctttacag
    20581 gcaaatagcc tttatccgca atcagagaat taattggtct aacgttaggg tcattggcga
    20641 gactagaagg aatcgtttga aaggaatcgc ctgtttccaa gttaatataa aaattctttt
    20701 ttactgctag ttcaaaatat ttttctgcat atacagaacc actaaagtga tgatgcaaat
    20761 ctccgccttt gggcattgca ttaaaaaagg ctgttaacat tgcctgattt ccacgaatct
    20821 ttttgaaata agcatttgtc ttttctgtat catttgcata aattaaagat tgatataagt
    20881 aaattggaat gatcgataaa aatagacttt tttttataaa tggaaacatt caatattatc
    20941 cttctattct tttgggagac tttttagaat ccttctctaa ttgctcccaa aaatgattac
    21001 tcattgtgat ttggtaaacc aactctattc aaaaaaaagc agagtcaagc ctatgtcaag
    21061 attattcgat agattccaaa ttggaatcag gtaagaattt tctaaagaat ccgatccaat
    21121 gccgaattta aaaagcaaaa cgagagatag aacaaaccat cacaaaacca actacatcca
    21181 tacaaaagaa aggaagtcat tttaaactta tttttgtaat acctagacat gcaggaatga
    21241 atatcagtct ttctctaaaa agacccaaat cttagattca aatcaaccta ctaacgctta
    21301 tattttgatt gccctttcag tgttttagca tattccaata tttcctttaa tttgtctgga
    21361 aaataatttt gaacaaaatc ctgatttttc ccaagtagaa attcttctat ggaacatgaa
    21421 cttccccatt ctccatgcgg attaccactc gtagccccaa aacaaaattc tttttcattt
    21481 aaggagcaag catattgcat tccatcttta tacaccgtcc attctttaag ttccattttt
    21541 tcctcttact gtgttaatcg caatttttga tttcaggata gccttaaaca aattcttgtc
    21601 tttccctaaa ttgacaatca aacagacttc tctttttttg ggatgcagtg tattatttaa
    21661 tattgtcaat agaaatcatt caaaataatc ttgctcaaaa ggaatatttt ctagatttat
    21721 catgcaccaa agctttccgt tgcatcattc cacacttctt gttgttctgg cagcagtcac
    21781 tttcattaca acaactttgc ttgttatagc tgccctcttt tcagataaaa ataaatctat
    21841 gatactttgg gcaaagggaa atgtattcat ctgccttggt tttgtggtgg gagcgttata
    21901 tttcattccc agttttttct attctgttat ttgttatgtt ttaatgggaa ctggtatggg
    21961 gtatatttat gcaggaataa agaaatttat caacgaagat gtgcatagaa gttttatccc
    22021 acttgtgtct aatttggctg gaattggacc tatgtattat ttatactggg acgatagtct
    22081 atcggggcga cttctcgtat cttctatcta cttcggaatt atcaatttaa tctgtgcttg
    22141 gactttctta aaaccatcta aaacatctga acgcggtgcg agtattttag cgggatctgg
    22201 atttttatgt atgggtttag ccttattgag tagaggtatt tttataatca aagcagattt
    22261 gaataatcct aaccatttcc aatccattga aactggaaca atatttctaa cttctatcct
    22321 tcaaatctgg acaacagttg ccttgattat attaattatg aaccgttatg cgaatcaaat
    22381 tctaaatatt tcaattacag acagtcttac aaatacattc aatcgaactg gtcttaattc
    22441 tctcggtctt gcaaaattaa agaaagcaga attgaatcaa attcctatgg caatcattct
    22501 attcgatgca gatcatttca aatcaatcaa tgatatgtat ggacatttga caggagataa
    22561 aatacttacc tatacagcag agaccgcaaa aaagaatcta agagagaagg acttaattgc
    22621 tcgctttggt ggggaagaat ttgtagtcat ccttgaagat gtatctagtc aagagagtta
    22681 tctcctctca gaaaaactgc gcgatgcaat cgctaagaat gaatacttta caggtaatga
    22741 atctgttcac tgctctatta gtatcggagt tgcctcaaca gaagtttgta atacatataa
    22801 tctttatgaa ctaatcgata ttgcagataa agctctttat aaagcaaaag cggaaggtag
    22861 aaatcgtgtt gttctttctg gataactttc aattctatcg gctgacgtct ataaactaat
    22921 agctgattca ataaaaaata aggagtcttc aaatttacta aatttactat atttattttt
    22981 ctatccgtct cattgattca atgttggaac tatagagcag actgttatga attagaagga
    23041 ttaaattatg ctgcatcaac aaagcctaat cgcttagaca ggactataga ttcttgtggc
    23101 gcacttgcct tgccttattt agtaaatcct tctgctaaaa tggattcttg gcaaagagat
    23161 actctacttt taaattgtta tctagtgaaa caaaaaagaa agaaatgtga taaagagccg
    23221 aatggaccga tacccgggaa tccttacatt tgggataaaa agtatggtaa ataatatttt
    23281 acttgagttc gatataacaa agaatttgaa agattaccac cgatggagac gataaacacc
    23341 gataggaagg aaaaattttc aaaccattga tagttaggag agcaagcgaa gtgacttgta
    23401 tgtatctgag gctcactctt tgttgaatct gttgaaaaag tatataatta caagaaaata
    23461 ttttccattt ccatgctatt cacgcatttt gtattttaaa aaattacatg gaacaattca
    23521 tgagtgaatt
//
LOCUS       DAOQOS010000087        23410 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_1038_length_23410_cov_5.537230, whole genome shotgun sequence.
ACCESSION   DAOQOS010000087 DAOQOS010000000
VERSION     DAOQOS010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 23410)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23410)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23410
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1038_length_23410_cov_5.537230"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(149..736)
                     /gene="nadD"
                     /locus_tag="PLF66_17400"
     CDS             complement(149..736)
                     /gene="nadD"
                     /locus_tag="PLF66_17400"
                     /inference="COORDINATES: protein motif:HMM:TIGR00482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HMZ65871.1"
                     /translation="MIAIYGGSFDPPHRGHLGVIQKFWKLFPESKELLIIPNYISPFK
                     KAKGVDEKHLLDMLSILIQENQILNTKIEEFEIRKKKPSYTIDTVLEIQNRYPKEEIQ
                     LIIGMDNLTKFSLWKDYKKILEIARLLVFDRDITDNREYPEELGIYRDRIFIVSDFKI
                     DASSSEIREMDRTRWNEYITPEVLSYIKNEKLYGY"
     gene            complement(733..1986)
                     /locus_tag="PLF66_17405"
     CDS             complement(733..1986)
                     /locus_tag="PLF66_17405"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012388762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HMZ65872.1"
                     /translation="MTDSNEYTENIAKRASAAKREIRTLKTSTKNDLLKALADEILKK
                     KETIMRENEKDIKAAREKNLSPTLIDRLLLNEKRIESLATSVLEIAALPDPIGEVVRG
                     VHLPNGIELVSKRVPLGVVLVIYESRPNVTIDVGALSFKSGNIAILRGGSEAIYSNIA
                     LVTIFRNILKSKGINEDAVIFVDQTDRSHIVSLLSKSQDIDIVVPRGGEGLIQFVTEN
                     SKIPVIKHDKGVCNIFVDASADFEKTVQILLNAKTQRPGVCNAVENVCIHKDFPQKKE
                     LLESLKAKNVDLLLDEPLLSIFPDARLATNEDYALEFLDLRLSFKTVSGIEEAISFIE
                     KYSSGHSEAILSEDHKNIEKFLSQLDSAALFVNCSTRFHDGGEFGFGAEVGISTGKLH
                     VRGPMGLVHLTTTKTILRGDGQVRG"
     gene            complement(1988..2605)
                     /locus_tag="PLF66_17410"
     CDS             complement(1988..2605)
                     /locus_tag="PLF66_17410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65873.1"
                     /translation="MAEFPHIEASNADSLEEMGTFFFKSLNDFILFTSGYYQMVENPP
                     KAILPVLDNPDFIVEVAALETAAVSFITSLFQGRLMIPALAIEYFLNNRIQSEMIFSL
                     LKEKGVVPDEDTFLRCLSGIDPDNHSFLQVNDKMANGIIAGSALFARESIYKKFITLI
                     ETTPLGQMSITQNALNTLLSALTSYLETRSIAIATSNIYQLHEGK"
     gene            2714..4030
                     /locus_tag="PLF66_17415"
     CDS             2714..4030
                     /locus_tag="PLF66_17415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017851365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HMZ65874.1"
                     /translation="MDIKNPKQRQISTIELLEKMERQYKNIPMEAILKQDVLRLGINF
                     SPESLTGSEFKSKDYFIFSFDHIPVAEMKEGVAYKTPEEIKVSGGHFNLLPTVISTRN
                     NPDSPYVVRLRENKPCLFLGEVNLGNLEFPPIPAWYRHKTKTGKIPGEIAPVIEWGYL
                     IYLTVFRNCQYFGKEEECAYCDINHNYRQQKNAGRPYTGVKDIEDILEVMSWIDKEDT
                     VAKVYTITGGSVLTNLKKKNEVDFYLEYPTEIEKHFPNRWMGKLVAQAFEKEDCKKFA
                     DAGIKVYHPNYEVWDPKLFDKICPGKASWIGRETWIRRIVDSAEVFGPSYVIPNFVGG
                     VELSEPYGFATVKEAIDSTAEGLDFFMSKGIMPRFTAWCPEPYTTLGTQAGPPLEYFC
                     ELLTVWKNTFEKYNLPTPPGYGEPGAGKAVFSVSAFMDVIGYQGRR"
     gene            complement(4044..4676)
                     /locus_tag="PLF66_17420"
     CDS             complement(4044..4676)
                     /locus_tag="PLF66_17420"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65875.1"
                     /translation="MASLPDLNTVLFCGAFEGETDLLSKSPLLRVYNTGVGFMDTIFN
                     LQRYLTKNKEIKSIVFLGSAGAYRHSKRKIGDIVYSNKFVYRDIAEVKKMVKVPDVIG
                     KHLLTDSDPRFNSFIKIAKLIEVVTNSTNYVTLIDLEMEELVDSLFDVDVENMEAFPI
                     AYVATRLSLPFTAYFYVTNYVGANGSIDWNQNWRYGSTLLQKEVMKYILA"
     gene            complement(4669..7218)
                     /gene="mutS"
                     /locus_tag="PLF66_17425"
     CDS             complement(4669..7218)
                     /gene="mutS"
                     /locus_tag="PLF66_17425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002975056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HMZ65876.1"
                     /translation="MQKESDPLATPAMKQFAEIKSKHPDGILFFRMGDFYEMFMEDAI
                     TASAILDIALTKRQNQIPMCGIPYHATESYISRLINAKKKVVICEQIKSEDPNVKLMQ
                     REVVRIITPGTIIEENLLGSYQNNYFAVFLFDLRLTLVAFADISTGDLYYFSFNREDT
                     DSLFATIRKFDPKEIMLISEEESYWSNYHFDEICVTTILSKLEIDFSQIDKTVKFSSL
                     EILIRYIVERNFKKNGAFAFKNPVIMDDSEYMELDEDTVRNLDLIENQNDASKHHSLF
                     SVLNKCQTGIGKRVLKNRILFPTKSKSVIESIWNQIQILDENKKIKNDLIQELSEFSD
                     IERIISRFRGGKGLPRDFRTIQKTIEISKSIADTLKKINYKFNIPESRLDSIYSFIGK
                     RLSENELPPILGGDGEFLKTGFDLKLDKAREAKIKGKDWIIELEEKERKSLGLNTLKI
                     RYNKVVGFYVELSRKDAELAPSRYHKKQTLVTSERFTFPELEEIERTILEADEIIQSI
                     EKEEFENMMKEVLKEFHSFIALSKEIGQLDFTLSLTECKNDYNWTLPNINDKGELSLV
                     ESRHPVIEKFMKVGERFVGNDVYLNRTNSSIAILTGPNMAGKSTYMRQIAICQILFQM
                     GSYIPAKKANLSIVDKLFTRIGSGDDITTGQSTFFVEMKETAHILKNKTSDSLVLFDE
                     IGRGTSTYDGMSLAWAILEKLSGETPLKTKTIFATHYHELTHLEKESGIFNLYMDTVE
                     EKEEIIFIKKVRMGKAKKSFGIYVAKLAGIPESIIQRAKVLLEGLESKKKEIKLKPIE
                     EPMLFPKVSEESTRHTKIIQSLEKIKIESITPMEAMQILNELQKMTRNSDG"
     gene            complement(7202..7828)
                     /locus_tag="PLF66_17430"
     CDS             complement(7202..7828)
                     /locus_tag="PLF66_17430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002176531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HMZ65877.1"
                     /translation="MTVRVKICGVKTIEIASICMDAQADFIGLNFSPESKRRVDLESA
                     FKIYNHVKKNSASLFVVGLFYKNSSAEIESIVKEIPFDFVQFVAHDETVEHDFYKGLG
                     KNLIPQISVKESISDSDLSLYKGELIILDSYKEGMGGGSGEVFNWDNVSSVRRKYLLA
                     GGLNPSNVERAVDVLNPFGVDVASGVESSPGVKDPKLIEEFIRNAKRI"
     gene            7955..8866
                     /locus_tag="PLF66_17435"
     CDS             7955..8866
                     /locus_tag="PLF66_17435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65878.1"
                     /translation="MKKTILLLLLSLLFCHKANETSNQSANTNPDPLLLKLNWYDGDL
                     SIKRKGAEIPLKSDLELEKDDLIHIKNGSAELLLGLENGIKLGSHTEISFSNNLVGDG
                     KTITTITLTEGSVFLNIIEGSEEKIKLITPNTKANLSNASMIAIVQKTGNKTIPCDKN
                     SCETKFYALNGSALLSLEENTNELKLEKNFEIKIGKETELTESLLTPINKKLIPNLAS
                     LVSFNTTDTKVDPKLLEEWISLPNSKMEIKKEIGKQTISPVKQAKLPIKKTEPKKLEI
                     KKQTPVKKQPAKQTNRDKLKLEANKKF"
     gene            complement(9012..11345)
                     /locus_tag="PLF66_17440"
     CDS             complement(9012..11345)
                     /locus_tag="PLF66_17440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65879.1"
                     /translation="MQANRSVKKYSSNPMAEPNSKNNNENVAAPSIPTKATVSDKTVL
                     VKTPQSMNSLSATSRCIMDELRDRHKGKDRPGSKPLHPTELITLDRIIDHLHKYSNLS
                     EQAKSNASIDQALRQLTDADNNGRERMAYLYPFLIKSGVKVIAKIALISPQYTHEINV
                     KNYRETCFDYSRAMVDLILSNRAKRIKEIDENNPGNFLFQKNKSGALWLNPNNCSLEA
                     EIGHLLKGGFKPYSYIPIKDFIDDFIEYGNQKGVLEMVMPGYHMIIDEIELKDDGSYL
                     RPPEIVNHYRAQADGLEKFALEQLRKLAEEGNLSDFKYKLNEYKREHVDLAPDGRRQS
                     REKVKVLLDLVKSFPFEKFNSEFSRSVQNTCNLSVRIVEKFMSDMNNLIDRKYSSIYN
                     KLSKSLEKLITEHTKNELTILNLDLNEEVERAGIKDPEKVKSFSERLKKDISNNFGSY
                     ATKDDSGKQAFYVVDQSYMASVLHKMASIAVSNPEYKKELEFAKIINQELTLQKNPKL
                     NTNIKEDHVSKLTSDMNTLEQQERERLKREAFQRKFNLPVGLMVFIPIMAIFLIISFV
                     EASLAAILFGLVISGGAGLAAAIYFRKKDQPSDKKNQSKQGMFGSEIMTDTAMQKAIE
                     EDIKGKEDKTSIIARAAIHHVFPKKYNTIEEKLYDINMLRTRIQSSLDDIKKSVPILA
                     KETDNQKVASAIEYSLMTNSVVIAIPADVVPRGKPASVIIAKEDFKSPLMRNQMSEYY
                     RALAEKSKYDSGLLKYYTFLINTIEVEYYKFLNKKFQ"
     gene            complement(11450..11998)
                     /locus_tag="PLF66_17445"
     CDS             complement(11450..11998)
                     /locus_tag="PLF66_17445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65880.1"
                     /translation="MLHKSIFLNWLKTKIDGLSKEDESEHVQLECSLIRAISEYREKE
                     LGQMHLSKDFDLRLMNRLETVEMEVVETYRDGSTRRYRMPLVFSAGFAVLAVAISTGL
                     YFSESSSPTFTKYPATVTPLDLKENSFEMSAEQRALLQELRKNPENVKIMEKLESYYY
                     ENGRKEQATEIHSLLETVSKKY"
     gene            complement(11988..12563)
                     /locus_tag="PLF66_17450"
     CDS             complement(11988..12563)
                     /locus_tag="PLF66_17450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012389122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HMZ65881.1"
                     /translation="MTLREKEKILLQKIKAGDKYAYMELVTPFKERLFRKAKSMVKDE
                     DDADDIVQDALIAGYKSIANFRAEAGVYTWLYRIVINKSKDLLTKRKRMVEKSIEDND
                     QQFVDERTGFEKKLELSDESTYLIKKINQLEDIYKEVIELRYFEEMSYSDIANLLECN
                     VGTVKSRLFKAKEILKHMILQDERGEVKIAT"
     gene            12880..14334
                     /locus_tag="PLF66_17455"
     CDS             12880..14334
                     /locus_tag="PLF66_17455"
                     /inference="COORDINATES: protein motif:HMM:NF021379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2079 domain-containing protein"
                     /protein_id="HMZ65882.1"
                     /translation="MSFYFILKARKKSVELEKNSNSKLEFFFALGLIFFYSLLFLYTF
                     YQFKNSFFLVDFDYLALAEILNNSLSGKFFTTHHYGNNVTGNYLAHHFSPSLLLLSPF
                     LFLSDTRLGYGYGLLFYTLLSLGLMGFLLYKFKLRGSVFFFSFLSLSINIYLYRLFFA
                     YHFELLIVFFFLLFFLGREINSKGLLFISILFLLLLKEDMAIYVSCLSLYFFLLRDWK
                     TGGLLLLASLFYFFYIPSFFRNYIDISTQVNWLEDWSKWGNTYSEIFFNILLNPISIF
                     KLFLDKWKVLKEFIFSFSPFVFLEWKLIIVSIPIFIIHFISDRIWYNSFYNYYSYTVI
                     SFWIVAFLISIKKMNESKWRQYLPGLLLLSLSISLYTNSDDQLFPYTRFQIDKTRLEN
                     IQTIVNFIPEKNSVATQFDLGAFVKRENPLFPLHEKNLDKDYILIDREKGITPYVDRK
                     RIETMIISMEGKYSLIAKYDGIELYKKINTDKPR"
     gene            complement(14396..14749)
                     /locus_tag="PLF66_17460"
     CDS             complement(14396..14749)
                     /locus_tag="PLF66_17460"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HMZ65883.1"
                     /translation="MIDLSYFIKLSNNKISFLIRVMNVFIDDTPKDIEELQISFNAKN
                     YKKVKEVCHKLKGLFKSYNMLELTQLTVELENNAKQENLGEGSIKLLNQILSNYEILK
                     SEMIILRDDYKKSEK"
     gene            14847..15740
                     /locus_tag="PLF66_17465"
     CDS             14847..15740
                     /locus_tag="PLF66_17465"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HMZ65884.1"
                     /translation="MKKNRIILFYIFILSTTIVCSEESKLKQKEKKQNNYTIAEELVT
                     EVKSNYSHIRVKDYGSLRQLYFVRDNGYEALESTIDIHYPQNLFLLYTQTMFASFLIN
                     KEHKDTLLVGLGGGGMVHFLNFYFPSISLDVVEIDPIILELAKKYFYLKNHPSTKISI
                     DDATNFISNTNKKYDVIFMDAFLKPSNETDSTGVSVKLKTVSFFQNLKSHLKKNGIVV
                     FNINTHDKIKNDVETILSEFPQVYFFQKDRSGNLIVIGSTDSNRISQLKMSEIAKDLD
                     KNHKVNFSFSDLASYYREKFE"
     gene            complement(15845..16366)
                     /locus_tag="PLF66_17470"
     CDS             complement(15845..16366)
                     /locus_tag="PLF66_17470"
                     /inference="COORDINATES: protein motif:HMM:NF019235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1564 family protein"
                     /protein_id="HMZ65885.1"
                     /translation="MNQKSKLISNQKIAFPRENLNFTEFSSTLLIPERYFTLFQKKCL
                     TNGGVRGYIAYLLSRYQIFIANGLIPGYANVTTKYQEKGQNLHKVAFRPNPEDWAELK
                     LYRVSFGMSISAFLVYLLIADSVEYAEASSDLLVGVGISKISNFDLMAKVYLTNNRSN
                     YTIFFQYRKSHSH"
     gene            16530..17990
                     /gene="gatB"
                     /locus_tag="PLF66_17475"
     CDS             16530..17990
                     /gene="gatB"
                     /locus_tag="PLF66_17475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HMZ65886.1"
                     /translation="MIDYEVIIGLEVHAQLNTITKVFSPSPTVFGSSPNTQVSTVCYG
                     LPGALPVLNELALQKAIQAGLALGCEISLFTKFDRKNYFYPDLPKGYQISQFDKPICL
                     GGEITFTLKGKTEPTTIHLTRIHLEEDAGKLMHSSDSSINASYVDLNRAGTPLIEIVS
                     EPEIRSSDEAYAYLTTLKSILRYIQVSDCNMEEGSLRCDANVSIRPRGEKTFRTRVEI
                     KNLNSFKAVKQAIDYEVEWQKDQYARGLTFPQQTKLWDATLLKTMSMRSKEMAHDYRY
                     FPDPDLPTIVLKKEDIDILKSKLPELPAAKKKRFEVVLGLPPYDAEVLTAEREIADYY
                     EAALKVSGDAKKTSNWVKDEVLGIVNKENISISEFSIGPDRIGSLVKLIADGKISGKI
                     AKDVFEKMLTSPKTPTQIVEEEGLLVVRDDKEIERLVDLILERNPQAIEDYRKGKDRA
                     LGSLVGAVMKESKGKADPPTVNKLLLDKLGPLPPKA"
     gene            complement(18207..18572)
                     /locus_tag="PLF66_17480"
     CDS             complement(18207..18572)
                     /locus_tag="PLF66_17480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65887.1"
                     /translation="MKKLFLIVFLLASCMTEDKKDVSSNSQKLKNKDSSAIEITRLTE
                     ETSCDNWIEKTAKDIKEKSDLPVSGSLIVFKSENGECKFYHVNKLNKQDLDIFSDKIL
                     MDCRLTNNHCKKIALVSFK"
     gene            complement(18588..19022)
                     /locus_tag="PLF66_17485"
     CDS             complement(18588..19022)
                     /locus_tag="PLF66_17485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65888.1"
                     /translation="MTFIDQITLPIFITWIVGLILVFFRRDLDFLWKAFFLLIFLFYI
                     IQFHEEIFAGFYRFKYNYAKEIVSWIYGLGKATYYMLLFTWPLSLFRIYYSAAGYLSE
                     ITIRLQVGLNVVYWIGFYFYSIFQTKIDNFLMNTLVHFLRFN"
     gene            complement(19009..19989)
                     /gene="pdxA"
                     /locus_tag="PLF66_17490"
     CDS             complement(19009..19989)
                     /gene="pdxA"
                     /locus_tag="PLF66_17490"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004751093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HMZ65889.1"
                     /translation="MKSIIISAGDPSGISYEIFDKGFSFLKKNAKDYKILFIETQNCI
                     SSKYKNKNNQIQIDQVDDFFSKKSSFKSGIYFLSLNILKTKEKIQIGEPSILSGKCAY
                     TSLMIAMGIIRKYGGNLITLPLSKEWVIKAKIPKFTGHTEELADFFKKKTFMMMYGEK
                     LKVIPLTTHIPIQKVSNALKKVSTKELISAIQSNRLLNKPKIAICGLNPHAGENGKIG
                     LEEVSILNPMIKKLKKSGLNVSGPISADSLFIPEYSKKFDLILACYHDQGLIPFKAFE
                     GKNGVNVTLGLDFIRVSPDHGTAFDIAGKNKADPTSFIQCVKVLTQDDIY"
     gene            complement(19991..20782)
                     /locus_tag="PLF66_17495"
     CDS             complement(19991..20782)
                     /locus_tag="PLF66_17495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004506677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65890.1"
                     /translation="MALSADQIVELQKLQRLLSLLEKTLSASKNDEQKKRVSKDIQKF
                     KSKILAISPSGIPDNFHNAVSPLNTQNVSSQGGMQPAQAASNVLSNIAVMKISPHSHD
                     QEINLIATLINVMETEYIPVLGDAHTKFDFSHATERDTVLKHLENIRRTMKVLTETIE
                     EYAQSEKQDFKEQLGRMKNKQSRIFISEAGELFRAFRDFLEKVEADMESGGVVVMNLG
                     EKVHFNSRFERATILEGKDVGYCLKQFLEFTRAALDSVNLPMIKK"
     gene            complement(20788..21711)
                     /locus_tag="PLF66_17500"
     CDS             complement(20788..21711)
                     /locus_tag="PLF66_17500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016544530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraslipin"
                     /protein_id="HMZ65891.1"
                     /translation="MEYIVGIFWVLFALFIAFKFIRSVRIVPAQEVLIVERLGRYHQT
                     LNAGLHFLIPFIDKVTYSHTLKEQAIEVQPQICITKDNVQVKVDGVLYLKILDAQKAS
                     YGIEDYRFAAIQLAQTTMRAVIGTLELDKTFEARDSINHRIVEVIDSAAELWGIQVNR
                     YEIQNLTPPKTVLEAMEKQKTAELNKKAEISLSEGDRDSKINRSEGLKQEAVNKSEGE
                     MQRRINEAEGRAKEIEAIASATAKGIEAVANAINTKNGKEAVKLQIVQGFIKEFTSLA
                     KSNTEIVLPMNLTDIESITSSVVSIIDKEKG"
     gene            complement(21711..22646)
                     /locus_tag="PLF66_17505"
     CDS             complement(21711..22646)
                     /locus_tag="PLF66_17505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016544388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045121 - membrane raft [Evidence IEA];
                     GO_process: GO:0034982 - mitochondrial protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stomatin-like protein"
                     /protein_id="HMZ65892.1"
                     /translation="MGTTFTIIFIIAIIIVYKTFIIVPEQYAFIKERWGKYQDPPLTA
                     GFHFLIPIMDRIHYEHTLKEVAYDVPPQECITKDNVTVEVDGILYLKILDPAKASYGI
                     DNYLLATTQLAKTTLRSEIGKLNLDDTFSERDEINQNVVKQIDHATDPWGIKVTRYEI
                     KNITPPKQVLHSMEQQMRAEREKRAEITISEGEKAARINRSLGEKKEAINVSEGEKQK
                     RINEAEGRAKEIEFVANATATGIKVVAEAISNKGGEDAVDLQITQAYLESLGKILMSS
                     KTTVLPSNMANIVGAFEGLSKVTGSFPDSKKGDKK"
     gene            complement(22693..23214)
                     /locus_tag="PLF66_17510"
     CDS             complement(22693..23214)
                     /locus_tag="PLF66_17510"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HMZ65893.1"
                     /translation="MTFVLARLLVVLAILFILAYFLMVVIVPENSVFIQERLGRYTRT
                     LNPGVYFQVPIIEKIAFRFTQKEQIRIIKELVCTAANGSKVYIDAIFIFQIEDPVKAA
                     YTTENYIESVLIETENSIITEIQNYLPEEIIPKKYGINQSIVKNTGEICKNFGLKIIR
                     FEIQQMKRDAKIT"
     gene            complement(23215..>23410)
                     /locus_tag="PLF66_17515"
     CDS             complement(23215..>23410)
                     /locus_tag="PLF66_17515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002151001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HMZ65894.1"
                     /translation="VGRKGGRISFQGTDWDAVSMDLEIPKGSYVKILDRENLTYQVQS
                     ATQEEINQYLSSIKKVEEED"
BASE COUNT         7393 a         4145 c         3581 g         8291 t
ORIGIN      
        1 tattccagct aagtaagctt ttcttggata agaatatcca tttaaaacag aaaatttttc
       61 agcaagttct gctactgaaa gactatgatc aaatcttgta tgtgttactt catctggaac
      121 aatttttaaa aatctctcaa tttctttttc aatatccata taatttctca ttttttatat
      181 aagaaagaac ttcaggagta atatactcat tccaccgagt tcgatccatt tccctaattt
      241 ctgacgaact tgcatcgatt ttaaaatcag aaacaataaa aattctatct cggtatattc
      301 ccaattcttc tggatactct ctattatccg taatatctcg atcaaataca agaagccttg
      361 ctatctctaa aatcttttta taatctttcc ataaactaaa ttttgtaaga ttatccattc
      421 caatgatcag ttggatttct tctttgggat acctattctg tatttctaaa acagtatcaa
      481 ttgtataact tggttttttt ttcctaattt caaactcttc tatctttgta tttaaaattt
      541 gattttcttg aatcagtatg gacagcatat ctaagagatg cttttcatct acccctttag
      601 cttttttaaa aggtgagatg taatttggaa tgattaaaag ttcttttgat tctggaaata
      661 atttccagaa cttttgaatt actcccagat gaccgcgatg aggaggatca aagcttcctc
      721 catagattgc aatcatcctc tgacctgtcc atctcctctc agaattgtct ttgtagtcgt
      781 taggtgaacg agtcccattg gtcctcttac atgcagcttc cctgtagaaa ttccaacttc
      841 cgcaccaaag ccaaattctc ccccatcatg aaaacgtgta gagcaattca caaatagagc
      901 agcagaatct aattggctta aaaacttttc gatattctta tgatcttctg aaagaattgc
      961 ttctgagtgt ccggaagaat atttttcaat aaaggatatt gcttcctcaa ttcccgaaac
     1021 tgttttaaag gataaacgta aatctaaaaa ttccagcgca taatcttcat ttgtcgcaag
     1081 tctagcatct ggaaagatgc ttaacaatgg ttcatctaat agtagatcta cattctttgc
     1141 tttgagtgat tcaagtagtt cttttttttg aggaaaatct ttatgtatgc aaacattctc
     1201 aactgcattg catactcctg gtctttgggt tttagcattt agaagtatct gcactgtttt
     1261 ttcaaaatca gctgaggcat ctacaaatat attgcatact cctttgtcat gtttgataac
     1321 aggtattttt gaattctctg taacaaattg tataagccct tctcctcctc gtggaactac
     1381 aatatcaata tcttgtgatt tagacagaag tgatacgata tgagatctat cggtttgatc
     1441 aacaaatatc actgcatctt cattgatccc ttttgacttt agaatatttc gaaaaatagt
     1501 cacaagagct atattagaat agattgcttc gcttccccct cttaaaattg caatatttcc
     1561 agatttaaaa gacaaggctc ctacatcgat tgttacatta ggacgagatt catagataac
     1621 caaaactact ccaaggggca ctctcttact tactaattca atcccatttg gcaaatgaac
     1681 acctcttacc acttcgccta tcggatcagg aagagctgct atttccaaaa ctgaagtagc
     1741 caaggattca attctctttt catttaatag gagacgatca atgagggttg gagaaagatt
     1801 cttctctcta gctgctttaa tgtctttctc attttctcgc atgatagttt ctttcttttt
     1861 taagatttca tcagctagag cctttagaag atcatttttt gtgctagttt ttaaagtgcg
     1921 aatctctctt tttgctgcac ttgccctttt agcaatattt tcagtgtatt cattggaatc
     1981 agtcattcta ttttccttcg tgtaattgat aaatattaga agttgcaatt gcaatacttc
     2041 tagtttctaa atatgatgta agtgcggata acaaggtgtt aagtgcattc tgggtaatac
     2101 tcatttgccc gagaggtgtc gtttcgatta aagtgataaa ctttttataa attgattccc
     2161 gagcgaacaa agcagagcct gcgatgattc catttgccat tttatcattg acctgtaaaa
     2221 acgaatgatt gtctggatcg attccagata ggcatcttaa gaatgtgtct tcgtctggaa
     2281 ctacaccttt ttcttttaat aaactaaaaa tcatttctga ctgaattcga ttgtttagaa
     2341 aatattcgat agcaagggct ggaatcatga gcctcccctg aaaaagagat gtaataaaag
     2401 agacagccgc cgtttcgagt gctgccactt ctacaataaa atctgggttg tccaaaacag
     2461 gaaggatcgc cttaggtgga ttttccacca tttgataata gccacttgta aacaggataa
     2521 aatcatttaa agacttgaag aagaaagttc ccatttcttc taaagaatct gcattcgatg
     2581 cttcaatatg tggaaattca gccattttta taacctatcg ctagatttct ttttgtgtca
     2641 aaaaaataaa ctagaaaata attctagctt gctttgtaac aaaaggcaaa aagtctaatt
     2701 ttgtatggca acaattgata taaaaaaccc aaaacaaaga caaatctcaa ccattgaatt
     2761 actcgaaaaa atggagagac aatataagaa tattccgatg gaggcaattc taaaacaaga
     2821 tgtgcttaga cttggaatca atttttctcc cgaatccctt actggctctg aatttaaatc
     2881 aaaggactat ttcatttttt cttttgatca tattccagta gcagagatga aagagggtgt
     2941 cgcctataaa actcctgaag agataaaagt ttctggtggc cattttaatc ttcttcctac
     3001 agttatctca actagaaaca atccagactc tccctatgtt gtgcgactga gagaaaacaa
     3061 accttgtctt tttttaggag aagtcaattt aggaaattta gaattcccgc cgattcctgc
     3121 ttggtatcgt cataaaacaa aaactggaaa aattccagga gaaattgcac cagtgattga
     3181 atggggatat ctcatctatc ttactgtttt tagaaattgt caatactttg gaaaagagga
     3241 agaatgtgcg tattgtgata taaatcacaa ctatcgtcag caaaaaaatg caggaagacc
     3301 ctacacaggt gttaaagata ttgaagatat tctagaagta atgagctgga tcgataaaga
     3361 agatactgtt gcaaaagtat atacaattac tggtggatca gttcttacaa atctgaaaaa
     3421 gaaaaatgaa gtcgattttt atttagaata tccaaccgaa attgaaaagc attttccaaa
     3481 tcgttggatg ggaaagttag tcgctcaagc gttcgaaaaa gaagattgta aaaaatttgc
     3541 agatgctgga atcaaagttt atcatccaaa ctatgaagtt tgggatccaa aactatttga
     3601 taaaatctgt ccaggaaaag cttcttggat tggaagagaa acttggatca gaagaattgt
     3661 agactcagct gaagtttttg gtccttctta tgtaatacca aattttgttg gaggagtaga
     3721 actttcagaa ccatatggat ttgcaactgt gaaagaagca atcgattcta ctgcagaagg
     3781 actcgatttt ttcatgtcta aaggaatcat gcctagattt acagcttggt gccctgagcc
     3841 atacacaacg cttggaacac aagccgggcc acctttggaa tatttttgcg aacttttaac
     3901 agtatggaaa aatacatttg agaaatataa tctaccaact ccacctggat atggggaacc
     3961 gggcgctgga aaagccgtat tttccgtatc tgcttttatg gatgttattg gttatcaggg
     4021 gagaagataa tatagattag ccactaagct aaaatatatt tcataacttc tttttgtaag
     4081 agtgtggagc catagcgcca gttttgattc caatcaatac tgccattggc tccaacataa
     4141 tttgtaacat aaaaataggc tgtgaatgga agagagagcc gtgtagctac ataggcaatg
     4201 ggaaaagctt ccatattttc tacatccaca tcaaataagg aatccactaa ttcctccatt
     4261 tccaaatcaa tcagtgtaac atagtttgta gaatttgtta ctacttcaat gagttttgct
     4321 attttaataa aagagttaaa ccttggatct gaatcagtta gtaagtgctt tccgatcaca
     4381 tctggaactt ttaccatctt ttttacttct gcaatgtctc tatatacaaa tttgttggag
     4441 taaacaatat cccctatttt acgtttggaa tgacgatagg cgcctgcaga acctaaaaag
     4501 acaatagatt ttatttcttt attttttgtc agatatctct gcaaattaaa aattgtatcc
     4561 atgaacccta cgcctgtatt ataaactcta agaagtggac tttttgaaag taagtctgtt
     4621 tctccctcaa aggctccaca gaataaaacg gtatttagat cgggaagact agccatctga
     4681 attcctcgtc atcttctgaa gttcatttaa aatttgcatt gcttccatcg gagtaatgga
     4741 ttctattttt atcttttcaa gtgactggat aatctttgta tgccgcgtgc tttcttcgct
     4801 cacctttgga aaaagcattg gttcttctat tggtttaagc ttgatttctt ttttcttaga
     4861 ctctagaccc tctagtaaga cttttgctcg ttgaatgatc gattcaggaa ttccagcaag
     4921 ctttgccaca taaatcccaa aagatttttt tgcttttccc attctgactt tttttataaa
     4981 aataatttct tctttttctt caacggtgtc catataaaga ttaaaaattc cagattcttt
     5041 ttcgagatga gttaattcat gatagtgtgt agcaaatatc gtcttagttt taagcggagt
     5101 ctctcctgat aacttttcta aaatagccca agctaaactc ataccatcat acgtagaagt
     5161 ccctcttcca atttcatcaa ataatacaag actatccgaa gttttatttt ttagaatgtg
     5221 agcggtttct ttcatttcta caaagaaagt agattgacca gtagtaatat catcccctga
     5281 tcctatccga gtaaatagtt tatctactat agatagattt gcttttttag caggaatgta
     5341 agaacccatt tgaaagagaa tttggcaaat agcaatttgc cgcatatagg tagacttacc
     5401 tgccatatta ggtccagtta agattgctat agaagaattg gttcgattga gatatacatc
     5461 attgcctaca aacctttctc ctactttcat aaatttttca atcacaggat gacgagattc
     5521 tactaaactg agttcccctt tatcattgat atttggaagt gtccaattat aatcattttt
     5581 acattctgtg agagaaagtg tgaaatcaag ttgtccaatt tctttggaaa gcgcaataaa
     5641 actatgaaat tcttttagaa cttctttcat catattctca aactcttctt tctcaatgct
     5701 ttgaatgatt tcatctgcct ctagaattgt tctttcgatc tcttctaatt ctgggaaagt
     5761 aaatctttct gaagtgacta acgtttgttt tttatgatag cgagaaggag caagttctgc
     5821 atctttacga gagagttcaa cataaaaccc gactacttta ttgtatcgga tctttagagt
     5881 gttcaaacct agagacttac gttctttctc ttctaattca ataatccaat cttttccttt
     5941 tatttttgct tctcttgctt tatctagctt cagatcaaat ccagttttta aaaattcacc
     6001 gtctccccca agaataggag gaagttcatt ttctgagagt ctttttccta taaaggaata
     6061 aatagaatct agtctagatt ctggaatatt aaatttataa ttaattttct taagtgtatc
     6121 agctatcgac ttagatattt caatagtctt ttgaattgtc ctaaaatctc tcggaagtcc
     6181 cttgcctcca cgaaagcgag agataatcct ttcaatatca ctgaattcag aaagttcttg
     6241 gattagatca tttttaattt ttttattttc atctaagatt tgaatttgat tccagattga
     6301 ttcgattaca gatttactct tagtaggaaa taaaattcta ttttttaata ctcgtttccc
     6361 aattccagtt tggcatttat ttaatacact gaagagggaa tggtgcttac ttgcatcatt
     6421 ttggttttct attaaatcaa gatttcgaac agtgtcttca tctaactcca tgtattcact
     6481 gtcatccatg atgactggat ttttaaacgc aaaggctcca ttctttttaa aattacgttc
     6541 tactatataa cggatcaata tttcaagact agagaattta acggttttgt ctatttgcga
     6601 gaaatctatt tctaatttcg aaagaatcgt agtcacacaa atttcatcaa aatgataatt
     6661 ggaccaatag ctctcctctt cagaaattag catgatttct tttggatcaa attttcgaat
     6721 tgttgcaaat aaagaatctg tatcttccct gttaaaacta aaataataca aatcacctgt
     6781 agatatatca gcgaatgcaa caagcgttaa acgcaaatca aacagaaata ctgcaaagta
     6841 attattttga tatgatccaa gtaagttttc ttcgatgatt gtgccaggtg tgatgattcg
     6901 aaccacttct ctttgcatga gtttaacatt tggatcttct gatttgatct gctcacaaat
     6961 gactactttc ttctttgcat tgataagcct tgagatataa ctttctgttg catggtaagg
     7021 aattccacac attggaattt gattttgcct tttggtaagt gcaatatcta agatagcaga
     7081 tgcagtaata gcatcttcca taaacatttc atagaaatca cccattctaa aaaacaaaat
     7141 cccatcggga tgtttggatt tgatttctgc aaattgtttc attgcaggtg ttgcgagtgg
     7201 atcagattct ttttgcattt cgaataaatt cttctattaa ttttggatct ttgaccccag
     7261 gacttgactc aacccctgat gcaacatcaa caccaaaggg atttaaaacg tcaaccgctc
     7321 tttctacatt ggaaggattt agaccaccgg ctaggagata tttccttcta acagaggata
     7381 cgttatccca attaaagact tccccacttc ccccacccat tccttcttta taactatcta
     7441 gaattatcaa ttccccttta taaagagaaa gatctgaatc agaaatagat tctttaactg
     7501 aaatttgggg aatcaaattt tttcctaacc ctttataaaa atcatgttct accgtttcat
     7561 cgtgagctac aaattgaaca aagtcaaatg gaatttcttt aactatagat tctatttcag
     7621 cggaagagtt tttataaaat aacccaacaa caaaaagaga agccgaattc ttttttacat
     7681 gattataaat cttaaaagct gattctaggt ctactcttcg tttgctctcg ggagaaaaat
     7741 tgagaccaat aaaatcagct tgtgcatcca tgcatatgga cgctatttca attgttttta
     7801 ctccacaaat tttcactcga acagtcattc tggaaacatt acagagaaag cctttcgaga
     7861 caagtttttt gttttttatt ggagactttt ccaattctat ttcgcccttt tttttattgt
     7921 aggaatagta agttgtagga tatgtggaaa gagaatgaaa aaaactatat tactcctact
     7981 tttatcccta ttattctgtc ataaagcaaa cgaaacttct aatcaatctg caaatacaaa
     8041 tccagatcca cttctactga aattgaattg gtatgatgga gatttatcga ttaaaagaaa
     8101 aggagccgaa atccctctga aatcagattt agagttagaa aaggatgatt taattcatat
     8161 taaaaatggt tctgctgaac ttcttttagg attagaaaat ggaatcaaat taggcagtca
     8221 tactgaaata tcatttagca ataatctagt tggagatggg aagaccatta caacgataac
     8281 actaacggaa ggttctgttt ttttaaatat tatagaaggc tcagaggaaa aaattaaatt
     8341 aataactcca aataccaaag cgaatctatc taatgcctct atgatcgcga tagtgcaaaa
     8401 gacagggaat aagacaatac cctgtgacaa aaacagttgt gaaaccaagt tttatgcttt
     8461 gaatggatct gcgctattat ctttagagga aaatacaaac gaactaaaat tagaaaaaaa
     8521 ttttgaaatt aaaattggaa aagaaacaga actcacagaa tctttgctta ctcctattaa
     8581 taagaagtta attcctaacc tagcatcact cgtatcattt aacacgacgg atactaaagt
     8641 ggatccaaaa ctattagaag agtggatttc tcttcctaac tcaaaaatgg agattaaaaa
     8701 agaaattggc aaacaaacaa tatcccctgt aaaacaagca aagcttccaa ttaaaaagac
     8761 agagcctaag aaattagaaa taaaaaagca aactccagtt aaaaaacagc ctgctaagca
     8821 aaccaatcga gataaactaa aactagaagc aaacaagaag ttttaatcct tacaaattta
     8881 tagttttaat tcattagcta tcctttagat agctttaagc tcttagatct ctttgtttta
     8941 atgattcaac tctttggatt caattgaagt atggttccga gtgaaaaaaa ggcaccaata
     9001 gaaaaggttt tttattgaaa ctttttattg agaaacttat aatactcaac ttcgatagta
     9061 ttgattaaaa atgtatagta ttttaaaagt ccagaatcat atttactctt ctctgcaagt
     9121 gctctatagt attcagacat ttggtttcgc atcaatggtg atttaaaatc ttcttttgcg
     9181 ataatgacag aggcaggttt acctctcggc acaacgtcag ccggaattgc aatgactaca
     9241 ctatttgtca tgagagaata ttcaatagct gaagcgactt tctgattatc tgtttctttt
     9301 gcaaggattg gaactgattt tttaatatca tccaaactac tttgaattct tgttcggagc
     9361 atattgatat catacagctt ttcttctatc gtattgtatt tttttggaaa tacatggtga
     9421 atggcagctc tggcaatgat acttgtttta tcttctttac ctttaatatc ttcttctatc
     9481 gctttttgca tcgctgtatc tgtcattatt tcagagccaa acattccctg tttagattga
     9541 tttttcttat cagatggttg atcttttttt ctgaagtaga tggcagctgc aagtcccgct
     9601 ccaccactga ttacaagtcc aaacaggatt gctgcaagac ttgcctcaac gaatgagatg
     9661 ataagaaaga tagccataat tgggataaat accattaacc ctactggtag attaaatttt
     9721 ctttgaaatg cttctctctt taatctttcc ctttcctgtt gctctaatgt gttcatgtca
     9781 gaagttaatt tgctaacatg atcttctttt atatttgtat ttagttttgg atttttctga
     9841 agcgtcagtt cttgattgat aatttttgca aactctagtt cttttttata ttctggattt
     9901 gagaccgcaa tggatgccat tttatgtaga acacttgcca tataactttg gtcaacaacg
     9961 taaaatgctt gtttaccgct gtcatcctta gtagcatagc ttccaaaatt atttgaaata
    10021 tcttttttta atctttcgga aaatgatttt actttttctg gatcttttat gccagctctt
    10081 tccacttctt catttaaatc taaatttaaa atagtaagct cattttttgt atgctcagta
    10141 atcaattttt caagtgattt tgacaactta ttatatatag aagaatattt tctatcgatg
    10201 agattattca tatcactcat gaatttttct actattcgca cagatagatt gcatgtgttt
    10261 tgaacagatc gtgagaattc ggaattgaat ttttcaaacg ggaaagactt aaccagatct
    10321 aatagaactt taactttttc tcgcgactga cgtctaccat ctggagccaa gtctacatgt
    10381 tctcgtttgt attcatttaa cttgtattta aaatcactta gattgccttc ttctgctaat
    10441 tttcggagct gttctaaggc aaatttttca agtccatctg cttgtgctct atagtgatta
    10501 acgatttcag gaggtcgcag ataactgcca tcatctttta attctatctc atctattatc
    10561 atatggtagc caggcattac catttctaat acaccttttt ggtttccata ttctatgaaa
    10621 tcatcaatga aatccttgat tgggatataa gaataaggtt taaatccgcc tttcaataaa
    10681 tgacctattt cagcttccag agaacaatta tttggattta accaaagagc tcctgattta
    10741 tttttttgga aaagaaaatt cccgggattg ttttcatcaa tttctttgat tcgttttgct
    10801 cgattggata gtattaaatc taccattgct cttgagtaat caaaacaagt ttctctataa
    10861 ttttttacat ttatctcatg tgtatattga ggtgaaatga gagcaatttt cgcgataact
    10921 ttaacaccac ttttaattaa aaacggatat aggtaggcca ttcgttccct accattatta
    10981 tctgcatcgg ttaactgtct cagtgcttga tcaatggatg catttgattt tgcctgttca
    11041 cttaaatttg aatacttatg taaatgatca atgattctat ctagagtgat gagttctgtc
    11101 gggtgcaatg gcttagatcc aggacgatct ttccctttat gacgatcacg caactcgtcc
    11161 atgatgcaac gagaggttgc agaaagacta ttcatagact gaggtgtctt aactaaaact
    11221 gtcttgtcgc taactgtagc tttggtagga attgacggag cagctacatt ttcattatta
    11281 ttctttgagt ttggttctgc cataggattc gatgagtatt tttttacact tctgtttgct
    11341 tgcaagcatt aaataacaac aagaatggaa agtttttcaa ttcatgcagc taacagggat
    11401 ttaacggaga actcttaaga gcttttacac aggcaaaagc tctttaaaat tagtattttt
    11461 tagagactgt ttctaaaagg gaatgaattt ctgtagcttg ttctttccta ccattttcat
    11521 agtaatagct ttctagtttt tccattattt ttacgttttc aggatttttt ctcagttctt
    11581 gtaaaagtgc tctttgctct gcactcatct caaaactatt ttcctttaaa tctaatggag
    11641 tcacggtagc tggatactta gtaaatgtag gagaggaaga ctctgagaaa taaagacctg
    11701 tgctgatagc cactgctaaa accgcaaatc cggcggaaaa aacaagtggc attctatatc
    11761 ttcttgtgct cccatctcta tatgtttcta caacttccat ttctacagtt tccaaacgat
    11821 tcataagtct tagatcaaaa tctttgctta aatgcatttg acctaattct ttttctctgt
    11881 attcggaaat tgctcggatt agactgcatt ctaattgaac atgctcagac tcatcttcct
    11941 tagataatcc atcaatcttt gtttttagcc aattcaaaaa tatagattta tgtagcaatt
    12001 ttgacttctc ctctctcatc ttgtaaaatc atatgcttca atatttcctt cgctttgaaa
    12061 agtctactct ttacggtgcc aacattgcac tcaagcaagt ttgcaatatc tgaataggac
    12121 atttcttcaa aatatcttag ctcaatgact tctttgtaaa tatcttctaa ttgatttatc
    12181 ttttttatta gatacgttga ttcgtccgac agttctaatt ttttttcaaa accagttctt
    12241 tcatctacaa attgttgatc attgtcttca atcgattttt ctaccattct ttttcgtttt
    12301 gtcaagagat cttttgattt attgatcaca attcgataca gccatgtata gacaccggct
    12361 tcggctctaa aatttgcgat agacttatag cctgcaataa gcgcatcctg cacaatatca
    12421 tcggcatcat cttcatcctt caccatagat tttgcctttc ggaatagtct ttctttaaaa
    12481 ggcgtaacca attccatgta ggcatattta tcccctgctt ttattttttg taacaggatc
    12541 ttctcttttt ctcttaaggt catctatgaa tacctataaa attaataaat cttttcagac
    12601 aatatatttt ctctgagagg agtctctact cacactattt ataaaggaat atttttgcta
    12661 ctatttttta taatgattct aatctcattt attctaataa atcagttctt aaaacttaga
    12721 aaagagtcgt cttttggctg tgtagatatt tctagtatta attgaatgct ttcttttctt
    12781 ttatcttttc ttctctttat aataacgttt ttcctgccat ttatatttca gtcccataca
    12841 cgtttgattc taagaataat ctccttatta gtttttttaa tgagctttta ttttattttg
    12901 aaggcgagaa aaaaaagtgt agaactagaa aaaaattcaa actctaaatt agaatttttc
    12961 tttgctttgg gtttgatttt tttttactcc ttattatttt tatatacttt ctatcagttt
    13021 aagaattctt tttttctagt cgattttgat tatttggctc ttgcggagat attaaataac
    13081 agtttatctg gaaagttttt tacaacacat cattatggaa ataatgtaac tggaaattat
    13141 ttagcacatc atttttctcc ttctcttctt ttgttatctc cttttctttt cttgtctgat
    13201 acgagattag gttatggata tggtctgctt ttttatactc tcctttctct tgggcttatg
    13261 ggatttttac tttataaatt taaattaaga ggatctgttt tctttttttc ttttctctcc
    13321 ctttcaatca acatttatct ttatagactt tttttcgctt atcactttga attactcatt
    13381 gtatttttct ttttgctttt ctttttagga agggaaatta attcgaaagg tttattattt
    13441 atttctattt tatttttgct tttgcttaaa gaagatatgg caatttatgt ttcctgtcta
    13501 tccctttact tttttctttt aagagactgg aaaacgggag gcttactatt attagcctct
    13561 ctcttttatt ttttttatat tccctcattt tttagaaatt atatagatat ttcgactcaa
    13621 gtcaattggt tagaagactg gagcaaatgg ggaaatacat actcagaaat ttttttcaat
    13681 attttattaa atccgatctc tatttttaaa ttatttttag ataagtggaa agtattaaaa
    13741 gaattcattt tttcttttag cccttttgtt tttttagaat ggaaactaat cattgttagt
    13801 attccaattt ttataattca ttttatttct gatagaattt ggtataattc tttttataat
    13861 tattactcat acacagttat ttccttctgg attgttgcat ttttaatctc aatcaagaaa
    13921 atgaacgaat ctaaatggag acaatatctt cctggacttc ttctcctctc tctttcaatt
    13981 tctctctata caaacagtga tgatcagcta ttcccttata cccgattcca aatcgataaa
    14041 acaagacttg aaaacattca aactatagtc aattttatcc cagaaaagaa ctcagttgct
    14101 actcaatttg atcttggtgc ttttgttaaa agagaaaatc ccttatttcc acttcatgaa
    14161 aaaaatctgg ataaagacta tatacttatc gatagggaaa aaggtattac accttacgtt
    14221 gatagaaaga gaattgaaac aatgattata agcatggaag gaaagtattc attaatcgct
    14281 aaatatgatg gtatcgaact ctataaaaaa attaatactg ataaaccaag ataagaagag
    14341 gaaacaaaaa ataaatttat ttcactctat tctttatttg tatttttatc tgttatcatt
    14401 tctcgctttt cttataatca tcacgcaaaa ttatcatttc actctttagg atttcataat
    14461 tggataaaat ttgatttaaa agttttatac tcccttctcc taaattttcc tgttttgcat
    14521 tgttctctag ctcaacagta agttgcgtca attccaacat attgtaactt ttaaacaaac
    14581 ccttcagttt gtgacacacc tctttcactt ttttatagtt ttttgcatta aaactgatct
    14641 gtaattcttc aatatccttt ggtgtatcat cgataaatac attcattact cgaattaaaa
    14701 aactaatttt gttgtttgat agttttataa aataagatag atcaatcatc ttgtcttatt
    14761 ctgtaaaaat cattttcaaa cgcaagcatt aaaagattta ccaagactta caggtctgta
    14821 gggtggctca aagagaggaa taggaattga aaaagaatcg cattattcta ttttatatat
    14881 ttattttatc aactacaatt gtctgctctg aagaaagcaa attaaaacaa aaagaaaaaa
    14941 agcaaaataa ctatacaatt gcagaagaat tagtcaccga ggttaagtcc aattattcac
    15001 atattcgagt gaaagactat ggatctttgc gacaacttta ctttgtgcgg gacaatggct
    15061 atgaggctct tgaatcaacc attgatattc attatcccca aaatcttttt ctactttata
    15121 ctcaaactat gtttgcctct tttctaatca ataaagaaca taaggataca cttttagtag
    15181 gattaggtgg tggtggtatg gttcattttt taaattttta ctttccatct atttctctag
    15241 acgttgtaga aattgatccg attattctag agcttgcgaa aaaatatttc tatttaaaaa
    15301 accatccatc tacaaaaatt tctatcgatg atgcaacaaa cttcatatca aatactaaca
    15361 aaaaatatga tgtcattttc atggacgctt ttttaaaacc ctccaatgag acagactcaa
    15421 ctggtgtatc agtcaaatta aagactgttt ccttttttca aaacctgaaa tcacacctta
    15481 aaaaaaatgg aatcgttgta tttaatatta atacccacga taaaataaaa aatgatgtag
    15541 aaacaattct gtcggaattt ccacaagttt acttttttca aaaagatcgt tcgggaaacc
    15601 taatcgtcat aggatccaca gattcaaata gaatttccca actaaaaatg tccgaaatag
    15661 caaaggattt ggataaaaat cataaagtta atttctcatt cagcgactta gcatcttatt
    15721 atagagaaaa atttgaatag tattttgaaa aatctaagca ttttatctat ttaattatag
    15781 cttcgcctaa ttgatttttg aggaatctga aatcttatat tgaattgaga atatttaact
    15841 cgtgttaatg agagtgactt tttctatatt ggaaaaagat agtatagttg gaacgattat
    15901 ttgtaagata gactttggcc atcagatcaa aatttgagat ctttgaaatt ccgacaccca
    15961 ctaagagatc ggatgaggct tctgcgtatt ctacagagtc tgctattagt aggtagacca
    16021 aaaaagcaga gatagacata ccaaagctta ctcgataaag tttcaattct gcccaatctt
    16081 ctgggttagg tcgaaaggca actttatgga ggttttgccc tttttcctga tacttagtcg
    16141 tcacatttgc ataacccggt atgagaccat tagcaataaa aatttgatat ctagaaagaa
    16201 gataggcgat atatccacgg acaccaccgt ttgtaaggca ttttttttga aaaagagtaa
    16261 aatagcgttc tgggattaat aaagtagagc taaattcagt aaaatttaaa ttttctcttg
    16321 gaaatgcaat cttttggttt gaaatcagct ttgatttttg attcataata tcacctctaa
    16381 aaaatatgag gaaaaaagtt tgatgtgtgt gcagaaataa tcctcttttt atgatttttt
    16441 ctttcaaaaa cgagtaaaat ccaatgacag ttaacaattg gacaaatttt caaattgcta
    16501 aataagtagg ctctatactc ttagaattta tgatcgatta tgaagtaatt attggattag
    16561 aagtccatgc ccaactcaat accattacaa aagtcttttc acctagccct acagtgtttg
    16621 gaagttcacc aaatacgcaa gtatccacgg tttgttacgg attaccagga gcactaccag
    16681 ttttaaatga attagcttta caaaaagcaa ttcaggcagg tcttgcactc ggatgtgaaa
    16741 tatcattatt tacaaagttt gataggaaaa attattttta cccagattta ccgaaaggtt
    16801 atcaaatttc ccagtttgac aagcctattt gtctaggggg cgaaataacc tttacattga
    16861 aaggtaaaac agaaccgaca acgattcatt taacacgaat ccacttagag gaagatgctg
    16921 ggaaacttat gcattctagt gattcaagta taaatgcatc gtatgtagat ttaaatcgag
    16981 cgggaacacc attgatagaa atagtttccg aaccggaaat acgatcaagt gatgaggctt
    17041 atgcttattt aaccacattg aaatcgattt tacgttatat acaagtttca gattgtaata
    17101 tggaagaagg ttccctacgc tgtgatgcaa acgtttccat tcgccctaga ggagagaaaa
    17161 catttagaac tagagtagaa ataaaaaatc taaactcatt taaagcagtc aagcaagcaa
    17221 ttgattatga agttgaatgg caaaaagatc agtatgcaag gggattaacg tttccgcagc
    17281 aaacaaaact ttgggatgca acattactaa aaacaatgtc tatgcgatct aaagaaatgg
    17341 cacatgacta cagatacttt cccgatccag atctaccaac tatcgtatta aagaaagaag
    17401 atatagacat cttaaaaagt aaacttcctg aattgccagc agctaaaaag aaaagatttg
    17461 aagtagttct tggtttacca ccttatgatg cagaagttct tacagccgaa agagaaattg
    17521 cagactatta tgaagcggct ttaaaagttt caggagatgc caagaaaaca tccaattggg
    17581 taaaagatga agtattagga attgttaata aagaaaatat ttccatttct gaattttcca
    17641 ttggacctga tagaattggt tcgttagtta aacttattgc cgatggtaaa atttctggta
    17701 aaattgcaaa ggatgttttt gaaaaaatgc tcacaagccc aaagactcca acacaaattg
    17761 tagaagagga aggtttacta gtagttcgtg atgataaaga aattgagcga ctcgttgatc
    17821 taatcctaga aagaaaccca caagctattg aagattatag aaaaggaaaa gatcgggctc
    17881 taggatctct tgtaggagca gttatgaagg agagtaaagg gaaagcagat cctccaactg
    17941 tgaataaact actacttgat aaattaggtc ctctcccacc taaagcataa cttttgagag
    18001 agatcatcta aattctatta gtgagatagg attatcaaag aaatatttct atctcctttt
    18061 tagagatatt tacagaaata gaaatttaag cgcattctaa aacaggtgaa tatcttttca
    18121 aaatatcaaa atttcaattt aaactacact tctgctctaa ttcaattagg gcagaagttt
    18181 taatttatta atttttagaa atgaatttat ttaaatgaaa ccaaggcaat ttttttgcaa
    18241 tgattattag tcaatctgca atccattaga atcttatcag agaaaatatc taaatcttgc
    18301 ttatttaatt tattcacatg gtaaaactta cattctccat tttcagattt aaacacaatc
    18361 aaacttccag aaactggtaa atctgatttt tcttttatat cctttgccgt tttttctatc
    18421 caattatcgc atgaagtttc ctctgttaaa cgagtaatct ctattgcaga gctatcctta
    18481 tttttcaatt tttgagaatt agaggaaaca tctttcttgt cctctgtcat acaagaagcc
    18541 aaaaggaata ctattaagaa taattttttc ataaaacctt cctagaatca attaaatctt
    18601 aaaaaatgaa ccagtgtgtt cattaagaaa ttatcaatct ttgtttgaaa aattgagtaa
    18661 aaataaaatc caatccaata tacaacgtta agccctactt gtagtcgaat agtaatctca
    18721 cttagatatc cagcagcact ataataaatt cgaaacaaac tcaatggcca agtaaataga
    18781 agcatataat aagttgcttt acctaatcca taaatccaac ttacaatttc ttttgcataa
    18841 ttatatttaa aacggtaaaa ccctgcaaaa atttcttcgt gaaattgaat tatataaaat
    18901 aggaatatta aaaggaaaaa agccttccat aaaaaatcta ggtctcttct aaaaaaaact
    18961 aaaattagac caacaatcca tgttataaaa atgggaagag tgatttgatc aataaatgtc
    19021 atcttgggtc aataccttta cacattgaat aaaacttgtc gggtctgctt tatttttgcc
    19081 cgcaatatca aacgcagttc catgatctgg agatactctt ataaaatcaa gcccaagtgt
    19141 aacgttaact ccatttttac cctcaaaggc tttaaatgga attaaaccct gatcatgata
    19201 acaagctaga attaaatcaa attttttcga atattcggga ataaacaatg aatccgcaga
    19261 tattggaccg gaaacgttaa gtcccgactt tttcagtttt ttaatcattg gatttagaat
    19321 agatacctcc tctaaaccaa ttttgccatt ctcaccagca tgtggattta acccacagat
    19381 tgcaatttta ggtttattta agagtcgatt actctgaatt gcagaaatta attctttagt
    19441 ggaaactttc tttaaagcgt tacttacctt ttgaatcgga atatgggttg tgagaggtat
    19501 aacttttagc ttctccccat acatcatcat aaaagttttc tttttaaaaa aatctgccaa
    19561 ttcctccgta tgccctgtaa acttagggat cttagctttt ataacccact ctttacttaa
    19621 agggagtgta attaaatttc ctccatactt tcgtataatt cccatcgcta tcataagaga
    19681 agtatatgca catttaccac tcagaataga gggttccccg atttggattt tttcttttgt
    19741 ttttaaaatg ttaagactta aaaaataaat tcctgactta aacgaactct tcttggagaa
    19801 aaaatcatct acttgatcta tttgaatttg attgttttta tttttatatt ttgaactgat
    19861 acagttctgc gtttcaatga ataatatctt atagtctttc gcattctttt ttaaaaagga
    19921 aaatcccttg tcaaatattt cataggagat tccggatgga tctcctgcgc tgataataat
    19981 gcttttcatt ttactttttt atcattggaa gatttacgct atcaagtgct gcacgcgtaa
    20041 actctaaaaa ttgttttaag caatagccga catccttacc ttctaaaatg gtcgccctct
    20101 caaacctgga attgaaatga actttctcgc ctaaattcat aacaacaacc ccaccagatt
    20161 ccatatctgc ttccactttt tctagaaaat ctctaaatgc tctaaacaac tcacccgcct
    20221 cagatataaa aatacgagac tgcttatttt tcattctacc aagctgttct ttaaaatctt
    20281 gtttttcaga ttgagcgtat tcttctatcg tctctgttaa aactttcata gtgcggcgaa
    20341 tgttttcaag gtgctttaag acggtatctc tctctgtcgc atgagagaaa tcaaacttag
    20401 tatgtgcatc tcctaaaaca ggaatatatt ccgtttccat tacattgatt aaagttgcta
    20461 ttaaattgat ttcttggtca tgcgaatgtg gtgaaatttt cataacagct atattcgata
    20521 gaacattaga agctgcttgt gccggctgca ttccaccttg agaactaacg ttctgagtat
    20581 ttaaagggct aaccgcatta tgaaaattat ctggaatccc acttggtgag attgcgagta
    20641 tcttagattt aaatttctgg atatccttcg atactcgctt tttctgctca tcatttttag
    20701 aagcggacaa agttttttct aataaactta aaagtctttg taatttttgt aattctacaa
    20761 tttgatcagc agataatgcc atattaatta tcctttttct ttatcaatta tactcacaac
    20821 agaacttgtt atagattcta tatcggtcaa attcattggt aatacaattt cagtatttga
    20881 ttttgcaaga gaagtaaact ctttaataaa gccctgaacg atttgaagtt taactgcttc
    20941 tttaccattc ttggtattta tggcattggc tacagcctca atccctttcg cggttgcact
    21001 cgcaattgct tcaatttctt ttgctcgtcc ttctgcttca ttaatccgtc tttgcatttc
    21061 cccttctgat ttgttaactg cttcttgctt caaaccttcc gagcgattga ttttagaatc
    21121 tctatctcct tccgagagtg aaatttctgc tttcttattt agctctgcag tcttctgctt
    21181 ttccatagcc tctaaaacag ttttaggagg agttaagttt tgaatttcat atcgattcac
    21241 ttgtatgccc cagagttctg cagccgaatc aatgacttca acaatcctat ggttaatcga
    21301 atctctcgcc tcaaatgttt tatctagctc taaggttcca ataactgcac gcattgtagt
    21361 ttgcgctagt tgaatggcgg caaatctata atcttcaatc ccataacttg ctttttgagc
    21421 atccagtatc tttaaatata aaacaccgtc tactttaacc tgaacgttat ctttggtaat
    21481 acaaatctga ggttgcactt cgatagcctg ttctttcagt gtatgtgaat aagtaacctt
    21541 atcaataaaa ggaattagga aatgtagacc cgcatttaaa gtttgatggt atctccccaa
    21601 tctttccact atcaacactt cttgtgctgg cacaattcga acggaacgaa taaatttaaa
    21661 agcaatgaat aatgcaaaaa gaacccaaaa tattccaaca atgtattcca ttatttttta
    21721 tctcctttct tagaatctgg aaaagatcca gtcactttag ataaaccttc aaacgcacca
    21781 acgatattcg ccatattgga tggaagaacg gttgtttttg agctcattaa aatctttccg
    21841 agtgactcta aataagcttg tgtgatctgt agatcaacag catcttcccc acctttattt
    21901 gaaattgctt ccgccactac tttgattccg gtagcagtcg catttgcaac aaattcaatc
    21961 tcctttgctc gcccttctgc ttcattgata cgcttttgct tttctccctc agaaacattg
    22021 attgcttctt tcttctctcc aagagatctg ttgattcggg cagctttttc gccctctgag
    22081 attgtaatct ctgctctttt ttctctttcc gctctcattt gttgttccat tgaatgaaga
    22141 acttgtttag gtggagtaat attctttatc tcatatctag ttactttgat tccccaggga
    22201 tcagtcgcat ggtcaatttg tttcacaaca ttttgattga tctcatctct ttccgaaaaa
    22261 gtgtcatcta aatttaattt acctatctca gatctaagag tagtctttgc aagctgagtt
    22321 gtggcgagta aataattatc tatcccgtaa gatgctttcg ctggatctaa aatttttaaa
    22381 tataaaattc catccacttc aactgtaacg ttatcttttg tgatacattc ttgtggagga
    22441 acgtcatacg ctacctcttt caatgtatgc tcatagtgaa tgcgatccat aataggaatc
    22501 aagaaatgaa atcccgctgt aagaggtgga tcttgatatt ttccccaacg ttccttaata
    22561 aacgcatatt gttccggaac tattataaaa gttttgtaaa cgattatgat cgcaattata
    22621 aaaataattg taaatgttgt tcccatatta tctcctttaa tttaaaaaat aaatttaatt
    22681 cgcataactt gcttatgtaa ttttagcatc tcgtttcatt tgctgaattt caaaacgtat
    22741 tattttcaat ccaaagtttt tacaaatttc tcctgtgttc ttgacgatgc tttgattgat
    22801 accatatttt ttgggaatta tctcctcggg caaatagttt tgaatttctg ttattatact
    22861 attttctgtt tcaattaaaa cggattcaat ataattttct gttgtatagg cagcctttac
    22921 aggatcttct atttgaaata taaatattgc atctatatac accttactac cattagccgc
    22981 tgtgcatact aattctttaa taattctaat ttgttctttt tgtgtaaatc taaaagcaat
    23041 tttttcaatg attggaactt gaaaataaac tccaggattt aatgtccttg tatatcgtcc
    23101 gagtctctct tgtataaata cagaattttc aggaacgatc actaccatta aaaaataagc
    23161 caaaataaaa agaatggcaa gcacaacaag aagtcttgct aatacaaaag tcactcaatc
    23221 ctcctcttct acttttttta tagagcttag gtattgattg atttcttctt gggttgccga
    23281 ctgaacctga taggtgaggt tttctctatc tagaattttt acataactac ctttaggaat
    23341 ctccaaatcc atcgagactg catcccaatc tgttccttga aatgagatac gtccgccttt
    23401 tcttcctaca
//
LOCUS       DAOQOS010000088        23161 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_1052_length_23161_cov_5.757898, whole genome shotgun sequence.
ACCESSION   DAOQOS010000088 DAOQOS010000000
VERSION     DAOQOS010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 23161)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23161)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23161
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1052_length_23161_cov_5.757898"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1737
                     /locus_tag="PLF66_17520"
     CDS             <1..1737
                     /locus_tag="PLF66_17520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017851349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HMZ65895.1"
                     /translation="GFPDKNSCEEEKKRLNIPKLRKILTKNPTYAGRKFEHVDFTIRE
                     NFLAYIENIVIKGNKKTLDKVIRRELLIKPGDLFNSALVNRSRERIFNLGYFKEVNFN
                     TRPGSSETKMNLIIDVVEQPTGTISMGGGYGTISGFSIFTELGENNLNGTGQKVSGRI
                     EFGPLRKFFQLSWTEPWIYDKPWSLTLSMFYSSRIINVGAASITDTQTQSIKERASYE
                     TSGVGFSVGVGHRLSFNGLLSNWTHFHRYSPSFFRSSNATSLVADTIRAQQDRGWQFR
                     SQITNGIGYDSRDNVFNPTSGINTYFSISNTGQFLGGQSHFDQFNPVIEFYHNWFDYT
                     LGGLIRSNALRRWKVVQEFRTSSVFTYERVPKYNRPDPILRESERQRNPYILQQDLQF
                     MGGYESLRGWFFQDNHYPREWQNGANHRVLASSELRFPIEPTLLWLVMFFDSGAMFED
                     VNRWTGQRKIDREAYDQLVQNQRWNTNIDQALNIYFRENYDPYSLKPLPISPLDLNNP
                     SRLVLSGNNIALDKFVYSWGFGLRIQIPVLPLRLYMAQRLRYTGVPEHPFTTYNDSKN
                     FQFVFGIGDIRF"
     gene            1740..3932
                     /locus_tag="PLF66_17525"
     CDS             1740..3932
                     /locus_tag="PLF66_17525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004751746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HMZ65896.1"
                     /translation="MEYPLLEKLNEEQKKAVLQTEGPVLILAGAGSGKTRVITHRIAH
                     LILDKGVSPYSICALTFTNKAAAEMKERVIQLIPNSYHGISVKTFHSLCLTLLRSNAD
                     LADLKPGFTVYDTSLQESLLKDIIKSMNLDTKVFIPSQIANRISHAKDSMILPDEFGI
                     DHRKDFYMDHVKEIYKKYEDRKKINNAVDFGDLILKTVLLFKNEPSVIRKYNLIWKYI
                     MIDEYQDTNKIQYELTKLLAGDHKNICVVGDDDQSIYSWRGADIRNILFFEKDFPDTF
                     TVKLEENYRSFSNIIQAASSVIQNNSERKDKKIFSNKPPGDKIRLTQFYSEHEEANAI
                     VKQIQKMYKEEKSYTKFVIFYRTNAQSRYFEESLRSASIPYKIFGGFRFFDRMEIKDV
                     IAYLSVIVNPGDDVSLLRIINSPPRGIGDTSLERIKDYGLSKGKSLLDSLNDDIPDIR
                     KATYKKMKELYNRFHDLIDLYKNGESPSKILIHMLESMGIHEHYQNEDSLESSDRLEN
                     IEQFVNAIKEYEENVENPSLSEYLNDIVLLTSEEEKKDLSDYVTLMTVHNSKGLEFDY
                     VFLTGMEEGTFPHRMSMESESEIEEERRLCYVAITRAKVKLFISHASFTRKFGMVEDR
                     IPSRFLKEIPVELFENSKTTEEFKYSPDYSPRAGDRKSNQDLDTKQRSISTNGNSFRV
                     GDRVKHKDYGVGKIMDISGSGDNLKVKIAFGYVHKNFLLAYTKLEKIT"
     gene            4012..6549
                     /locus_tag="PLF66_17530"
     CDS             4012..6549
                     /locus_tag="PLF66_17530"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing methyl-accepting
                     chemotaxis protein"
                     /protein_id="HMZ65897.1"
                     /translation="MGKLDSILNEEKTFAADCTLISKTNLKGVITSANQNFSNVSEFP
                     LEELIGQPHNIVRHPDTPAILFKDLWNKLKAGIAWNGIIKNRTKTGKYYWVDALVSPE
                     FDKNGEIVGYISVRRKPTREQIEAAEILYRDLNSGNTSFRKSLASQFSFIRRLRIGTR
                     LSLLLVIITFSLIAPSAVLIKLKMDDIDLSKKELVGSKINVSISEFIRTLSNHRGLYN
                     RFLSSKDESLLNDIELKEKELDGISKDLLSLLETEKSNGTTREDIQSITKKWQELKIK
                     NAQLDVTQNYEEHVSLINQTLTIMQKITEGYELLYDPYQDTFHFINLSNTKIIQIAEI
                     LGKIRNEGVLVLNSKNKTKESLTILEMRMTESQTFLNGIEGDIQYIFKYNPSLTDKYT
                     RLRDKSDSSITNLFQVVKEEIINKEYLTYTPQEYYNIATSALESIHDLNRELNTDLQN
                     KLNSRIDSQVHDIYFTILSLFILLIISISLTVIISQSINKPLQNIVKKVKLMVRGGGD
                     FTTKFDMNVEDEIGELAGWLNIFVLRITEIIYQIRRESSNLESQTKTAFNIADNFSKA
                     VSEQANFAEEASSSASLMRNAISNIFENVKEEAKHIEEINGFMDEFHAQIRNTQFSIE
                     HLQKVAQKLSEMAKLSSDLIEETNLSINQVNEKSASIDRIVDVIMQISNKTSLLALNA
                     SIEAARAGEMGKGFSVVAQEISTLAEQTAKNTKSIREITQGTKESIRKSVDQTSKTNE
                     NLKSLLVNIADIHVKSFELTSSQKKQKESSELIVKSLHEIEETSKEIVSSANEQKSAS
                     QAIADVLEMISNNTVKSSENSASLLEVAKQTNAVSGDLNKMIEQFKY"
     gene            6698..6904
                     /locus_tag="PLF66_17535"
     CDS             6698..6904
                     /locus_tag="PLF66_17535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65898.1"
                     /translation="MKILTKQKYFRFIFISFLLVANNTFATNPCFQVNAKNGLNLREP
                     REIQFFPTVIARRYAHSIIRIFND"
     gene            complement(7215..7730)
                     /locus_tag="PLF66_17540"
     CDS             complement(7215..7730)
                     /locus_tag="PLF66_17540"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HMZ65899.1"
                     /translation="MAYLFWNERSQTYGYSVSREAKDFFLEDQTNTKRSLKDFLGNKI
                     FLFFGYIQCKTACPPSIGAFLSLSKRLKEENVIFIFISIDPKRDKQNSYEFSKYFGDK
                     FFLLTGNQKQIEDVTNSYEIFPYKEIGSDEINHAGHIFLIDTSLRIRYIYPSNFRDIS
                     RIMEDFYKLDI"
     gene            complement(7792..9318)
                     /locus_tag="PLF66_17545"
     CDS             complement(7792..9318)
                     /locus_tag="PLF66_17545"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HMZ65900.1"
                     /translation="MNVDYRSAEEKYKEECYKLDLFFILFIGMHVPVSLLVSIGYGTI
                     YITLVCSLIVFFISLVAFFFFRGTTISRNVFALSVMLFSGILIEAQLGRIEMHFHVFA
                     ALAFFKMYMDYTTIITAALTIALHHIGFNLLQENNVTIGGIPLIAYNYGHGWGIVFLH
                     ACFVLLQSSLLIYFSITRKKRFMEIENLADLGRILEQNKITTRNLDDISIRIKEIVSS
                     LNNNSSTIQTASNEQSSSIHDISDAMREIVSTMESILDNVKKQSQKITSFTDLMNSLS
                     DLNRSVLNQLDHSNSIVEKTQMESEKEKATLSEMLNSLSKMDKAYEKMQTIISGIYEI
                     ADQVNLLSLNASIEAARAGEHGKGFAIVAREVSKLAEQTSNSIRESDSLMKIVKMELK
                     NSNQTIKTGADSFGKIISRFGEIQKVVNQFSGTVTDQIKSFSQLNSEIQFLRESGDQI
                     SNTVYEQKNSMQEILMAVEMINENTLGFNKLSAEILNSANEAESTVEKLKQEIKNLEH
                     "
     gene            9723..10892
                     /locus_tag="PLF66_17550"
     CDS             9723..10892
                     /locus_tag="PLF66_17550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65901.1"
                     /translation="MQNINKFIWVNSFFFSLKRSLFFIPLILTFVHFSNCVRFKIDNL
                     KPNLISKIKIGTDLTEVEGMVVNNALINIPFRIPINANRLYITDYNHSYIKVYGNDGD
                     LDFIIGNVPSKNNLKVKVLNQKLGKIGLVAVSSEDDLFIQNRISTDSGAEEIKAENSF
                     AKNSGYFDTKEKENTASYILHLDSKGKMVSIIGISGKNSEPFRYIESMYAYDKKRFAV
                     YHKFAEQMHLNYYVEDNLVGTIKEGNLNIFTGPDSNEYKIKLDSMIPHRSGEFALVSF
                     SFIGKKDLRFKFRRIYKYAFGSVEPEILLKEFQYPSEILFSVNTNKEFYIWETENKGE
                     SVKLQVHDKDGNHINNKRLQFPAPRLNWRETYVDENDNLYSVKTSGGYLELYSWY"
     gene            10985..11494
                     /locus_tag="PLF66_17555"
     CDS             10985..11494
                     /locus_tag="PLF66_17555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65902.1"
                     /translation="MFQYVVVGNIFALLFVLFVYSKENGFLNTSFKPSNKIQLEELEA
                     YFVRSNESTQEETSFFFFLKNQSQKETSFPKSEMQVIFTLTTEEKEECLQKKFELNPK
                     KILPKNTEFFPLLIEEKEIKKTKTCDTFFHKIQKKNLTDLFAKGNSKFTPELMVIYQG
                     QIYRMSINN"
     gene            11491..12162
                     /locus_tag="PLF66_17560"
     CDS             11491..12162
                     /locus_tag="PLF66_17560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008595799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HMZ65903.1"
                     /translation="MKLRSKKHPGQTWFATLSIYFFGSVILRLFYKIKIEGGENIPKE
                     GALIVLAKHQRLNDIPLGCAAFYVKARRRLWCIMKDSLSNPFLGGYLLKCGGIPIDRK
                     NPEKTKESFTFAKHVLHHNQVLTLFPEQTFFPWKMGRGKVPGFRVLAGKPAHPLQVIC
                     VGFEYGKEKWGRTSVTIRIGKVSYFTKEDSPELFLHEKMKEIASLSNLEYNFPVSTAK
                     EESEE"
     gene            complement(12345..12890)
                     /gene="bfr"
                     /locus_tag="PLF66_17565"
     CDS             complement(12345..12890)
                     /gene="bfr"
                     /locus_tag="PLF66_17565"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004789070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HMZ65904.1"
                     /translation="MKGNAEVIEILGEVLAAELTAINQYFIHAKINKNKGYTKLAKYM
                     QHESQRETGMSNILLCYTKLAKYMQHESIDEMKHADAVIERILFLDGTPDVQRYMKIM
                     VGSNIEEILKHDLALEYAAVERLNRGIEIATRLKDNGSRELMEKILVAEEEHIDWIEA
                     QQSIIAEIGVANYLAQQIETE"
     gene            13175..14278
                     /locus_tag="PLF66_17570"
     CDS             13175..14278
                     /locus_tag="PLF66_17570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012388774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HMZ65905.1"
                     /translation="MSNIYIHSPNLSKFGKQDLSVLELSRQTGNSSLSQFDRNRIDLI
                     LFTSFTPEKYNNEFHLAAKLADSLNIKTAFTIRTETASSSGASALQVGYSMLSSGLFK
                     NALIVGTEIMSRLGREESNLVLGSVLSDRMRNFSMSMAQGAAMITRRYLKDYDYTESD
                     LFYIAEKLHANGSENPNAHIRKKITREDYEKAPLFASPLGLYDISPLSDGSASLILST
                     EIKSDIRIRGIGHGLSGFKTMELTTSFPASLAAFSKAYSMAKIKPEDIKIAELHDAFT
                     TFEVIGAEDAGLFPKGHALNKVKEGVSSINGDLPINPSGGLKSRGHPIGASGLAQIAE
                     LIRFMRIQNKSLGLAHSIGGLATNNFATILERI"
     gene            14275..15321
                     /gene="waaF"
                     /locus_tag="PLF66_17575"
     CDS             14275..15321
                     /gene="waaF"
                     /locus_tag="PLF66_17575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004468043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HMZ65906.1"
                     /translation="MKILIIQTAFLGDLILTTSFFREVKQKYKEAEIHVIVNKGTEDI
                     LFGNPNITKVISFDKAKVRKSIFYFFSFIQCLRKEKYEICICPHFSYRSTLISFFSGA
                     KVRIGYKESGFSFLHTKKISRPLEGKHEVDKLFSLIHENTDSFSLKQKRPELFFSEAR
                     LIKIKTILETYHLNKNFIILAPSSIWETKRMPTSKFQELFQRIVADTDYSIVLIGSPK
                     DQILCENIIDRRLPENQVNKVERIFNLAGKTDLVELSYVISQALAIVSNDSSPIHFAS
                     AHNIPTIMIYGATVPAFGYSTLVDKQYLSQVDGLSCRPCGIHGGKVCPEKHFKCMEEQ
                     NIDIMFKQLIQLIK"
     gene            15640..16497
                     /locus_tag="PLF66_17580"
     CDS             15640..16497
                     /locus_tag="PLF66_17580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65907.1"
                     /translation="MNTNRFTQTDDFKFIPILSVPSVFTNSTVSVQVSGNFLNRLLHR
                     NDVKLKEVINLFFKNHLLHILSFLFLLIQCSSLEQERLAFEKDKLEFEKKKLQFEREK
                     IKSCGCCSPCNTCNGTAEVVVAEKKEKDCIKEVPPTSEDIWALSVEGNWHYAPDKIKN
                     KNICKGDLKETFYKQFANTESYVAEKQVPATKQSSCINNVLFSGKSQLYNLMVDKTAK
                     EESISISKELKAQVLQESIEYNNAEKGRSFYYECKPTDPNGRWDSCKCVLYVSYPEGK
                     QGLSARLKK"
     gene            16512..17609
                     /locus_tag="PLF66_17585"
     CDS             16512..17609
                     /locus_tag="PLF66_17585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002973130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HMZ65908.1"
                     /translation="MKYLSVLIIFSLVYCSAFGKDPEGTHLEKIKQSPQFDKERDQFV
                     NRRADILEKMRENQNFFKLTLLFFFGGEKHQRPPEKLPELKPDVKEFLEKSDKIKFIW
                     FGHSTFLVNFDGKIILFDPVFSNNASPISLFVKRFQPPVLKLEELPNIDYIIISHDHY
                     DHLDMETIEFFKSKSVKFITPLGVTSHLKEWGITEDRLTELDWWSSINRDDLEIILTP
                     AQHFSGRKGNNQNKTLWGSWIVRNKKQSLYFSGDSGYDTHFKAIGDKYGPFDITFIEN
                     GQYNERWRAVHVLPEETAQAFLDLKGKHLVPVHWGMFDLSLHNWYDPIERIEKASKEK
                     NIKLLTPKFGQIVKLEEDTIFETWWKKFIQE"
     gene            17817..19115
                     /locus_tag="PLF66_17590"
     CDS             17817..19115
                     /locus_tag="PLF66_17590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000205136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P N-terminal domain-containing
                     protein"
                     /protein_id="HMZ65909.1"
                     /translation="MANEIYKKRIKKVQSILKEGETLLLFAANHLIRNRDVEYKFRQD
                     SDYYYLTGINEPDGILVLKKNYSGLFVLPKDKEKEIWTGIRLGKKEAKKLLSLDETFD
                     TTEWNDKSIELLTNSDTLFYFFGKDKDRDSGLIALCDHLNKRSRIGQYGPSKIQIPEF
                     LHEMRMIKSKEEMDAILESAKITEKGHIALMQETKPNMYEYELEAILEREYLRHGAWG
                     GGYGHIVAAGGNATILHYTFNSAKIKKGDLILVDSGAEKNCYTADVTRVYPADKKFTS
                     PQKDVYEIVLDAQKKAIRNTVAGKQFLAIHNETVRDLIVGLKSLKLLRGSVDKIFETG
                     EYRRFYMHKTGHFLGMDVHDVGRYFEKGESRKLVNGMVTTVEPGLYFDPTDKTIPKHF
                     RGIGIRIEDDILVQGKTPINLTASIPKEVSEIEAIRETVD"
     gene            complement(19235..20482)
                     /locus_tag="PLF66_17595"
     CDS             complement(19235..20482)
                     /locus_tag="PLF66_17595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004281818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HMZ65910.1"
                     /translation="MTNIKIPRYPVLIVEDKKENQVLIQSICHQLGISTVIADNGRMA
                     LDLSKSRKFSLFIVDLMLPILDGKSFIEELKIIEPQAVIIIQSALDASETIINVMRLG
                     VFDYIIKPIDIEFFQHTLTKALEYKYLKDIEAELSLNESLKLRGQLEWLNYKETLRVS
                     GKDSYQKNSIVNLSTSLSQGGGVGVNISLLDMLKLDMIEQGDTYVVNKEILDTVFENN
                     EYSRRVLFGLGQVVDLMEQPFDFKEMEAFKLAGELPTYLSHILPYLKNKNTRVAFPNI
                     TSKSKLEIDIEKIKLVFEELLINSYKYSTPSSRVDIFAHVAGSYFCITFKNEIDEKSY
                     GGIPPEKEKLVLEPFFRLHPPVEDILEVEKFSLGLGLTVVDFIVNKHNGMFFIHNAID
                     HTGKRKTNCVLAEVFLPIKMEKI"
     gene            20999..21604
                     /locus_tag="PLF66_17600"
     CDS             20999..21604
                     /locus_tag="PLF66_17600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65911.1"
                     /translation="MSAKVMEQGFLNLEYQKEFGNYRLKLLLATDEIGIIYRVTSVLY
                     ANEWNILGANIRSLENGQIEDEFLIHHSNGINLDDEELNKLKSEMEQLFRDKISITSY
                     MIKKGKKINNQKGNEPAKISFETVANKDLTKLRIQTKDRSGLLCDISRMLFLECMDIL
                     SLQAKTRGDEVDDTFEIKSEAGHSLDETMQARLTRNLQNVI"
     gene            complement(21597..22571)
                     /locus_tag="PLF66_17605"
                     /pseudo
     CDS             complement(21597..22571)
                     /locus_tag="PLF66_17605"
                     /inference="COORDINATES: protein motif:HMM:NF016041.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
     gene            complement(22881..>23161)
                     /locus_tag="PLF66_17610"
     CDS             complement(22881..>23161)
                     /locus_tag="PLF66_17610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004462270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HMZ65912.1"
                     /translation="SQTAFGSSSADVLTKATRFIAISFIVVSLLLSFLFAKKEEAIIE
                     QLPTSEIVPAKEDAKQPADGTNQAPASQTAPTNATQPANPTPAAPAKQ"
BASE COUNT         8420 a         3739 c         3812 g         7190 t
ORIGIN      
        1 ggatttccag ataaaaattc ctgcgaagaa gaaaagaaac gtttaaacat cccaaagctc
       61 agaaagatat taacaaagaa tccaacctat gcaggacgta aatttgaaca cgtagatttt
      121 acaattaggg aaaacttcct agcttacatt gaaaacatcg taatcaaagg aaataaaaaa
      181 actttagaca aagtaattcg tcgtgaactt ttaattaagc ctggagatct tttcaattca
      241 gcgcttgtaa atagatctag agaaagaatt tttaacttag gctatttcaa agaagtaaac
      301 tttaacaccc gccctggttc gagtgaaaca aaaatgaatc taatcattga tgtggtagaa
      361 caacctactg gaactatctc tatgggaggt ggttatggaa caatttctgg attctcaatt
      421 tttactgaat taggagaaaa caacttaaat ggaacggggc agaaagtatc tggtcgtatt
      481 gagtttggac ctctaagaaa atttttccaa ctgtcttgga cagaaccttg gatttatgat
      541 aaaccatggt ctcttacttt aagtatgttt tattcttctc gaattatcaa cgtgggagcg
      601 gcctctatta cagataccca aacgcaatcg attaaagaga gagcgtcata cgaaacatct
      661 ggagttggtt tttcagtagg ggttgggcat agactttcct tcaatggatt gctatcgaac
      721 tggacacatt ttcatagata ctcacctagc ttttttagat caagcaatgc aacatcactt
      781 gttgcggata caattcgagc acagcaggat agaggttggc agtttagatc tcagattaca
      841 aatggaattg ggtatgattc tagagataat gtattcaatc caaccagtgg aatcaatact
      901 tatttctcca tttccaacac tggacaattt cttggaggac aaagtcattt tgatcagttc
      961 aatccagtta ttgaatttta tcataactgg tttgattata cgttaggtgg actcatccga
     1021 tccaatgcac tcagaagatg gaaagttgtg caagaattta gaacctcgag tgtatttact
     1081 tatgaacggg ttccaaaata caatcgacca gatccaatat taagagaatc agaacgccaa
     1141 agaaatcctt atattttgca gcaggattta caatttatgg gtggatatga atccttgcgt
     1201 ggttggtttt tccaagacaa tcactatcca agagaatggc aaaatggagc taaccatcgt
     1261 gtcctagcca gctctgaatt gagatttcca attgaaccta cattactttg gttagttatg
     1321 ttttttgact caggagccat gtttgaagac gttaaccgtt ggactggaca aagaaaaata
     1381 gatagagaag catatgacca gttagttcaa aatcaaagat ggaatacaaa tatagaccaa
     1441 gcactcaata tttattttag agaaaactat gatccttata gtttaaagcc cctaccaatt
     1501 agtccattgg atttaaacaa tccatccaga cttgttttat ccggaaataa tattgcattg
     1561 gataaatttg tatattcatg gggatttggt cttagaattc aaattccagt tctaccactc
     1621 cgactgtata tggcacaaag attgcgttat acgggagttc ctgagcatcc ttttacaacc
     1681 tataatgata gtaagaattt tcaatttgtg tttgggattg gagatattcg tttctaatta
     1741 tggagtatcc attacttgaa aagttaaacg aagaacaaaa aaaagcagtt ctacagaccg
     1801 agggacctgt gttaatttta gcaggggctg gatctggaaa aacaagagta atcacacatc
     1861 gaattgcaca tctaatttta gataaaggag tttctcctta tagcatatgc gcattaacat
     1921 ttacgaataa agcagctgca gaaatgaaag agagggtaat acaacttatc cccaactcct
     1981 atcatggtat atctgttaaa acatttcact ccttatgctt aacgctcctg agatccaatg
     2041 cagatcttgc tgatttaaaa cctggattta cagtctatga tacatctctt caagaatctc
     2101 ttttaaaaga tattatcaaa tcgatgaatc tggatactaa ggtttttatt ccctcgcaga
     2161 ttgcaaatcg tatcagtcat gcaaaggact cgatgattct tccagatgag tttggtattg
     2221 atcatagaaa agatttttat atggatcatg taaaagaaat atataagaaa tatgaagatc
     2281 gaaaaaaaat aaacaatgca gtggattttg gagatcttat actaaaaaca gttcttttat
     2341 ttaaaaacga accgagcgtt attcgcaaat ataatctcat ctggaaatat ataatgatag
     2401 atgagtatca agatacaaat aaaatccagt atgaattgac taaactctta gctggagatc
     2461 ataaaaacat atgcgttgtg ggtgatgatg atcaatcgat ttattcttgg agaggtgctg
     2521 acattagaaa tattctattt tttgaaaagg acttccctga cacatttact gttaagttag
     2581 aagaaaatta tagatctttc tcaaacatca tacaagctgc tagtagcgta attcaaaata
     2641 attcagaaag aaaagataaa aaaatttttt caaataagcc tcctggggat aaaataagac
     2701 taacacaatt ttatagtgaa catgaggaag ctaatgcaat tgtaaaacaa attcagaaaa
     2761 tgtataaaga agaaaagtcc tatacaaaat ttgttatctt ctatagaaca aatgctcaat
     2821 ctagatattt tgaggaatct cttagatcag cttctattcc ctataaaatt tttggcggat
     2881 ttcgattctt tgatcgtatg gaaattaaag acgttattgc ctatctatca gtcattgtaa
     2941 atcctggcga tgatgtatca cttcttagaa tcattaactc acctccaagg ggaattggag
     3001 atacttcctt agaaagaata aaagactatg gattaagtaa aggcaaatca ttgttagact
     3061 ctctgaatga tgatatccct gatataagaa aagcaactta taaaaaaatg aaagaacttt
     3121 acaatagatt tcatgatttg attgatttat ataaaaatgg ggagagccct tctaaaattt
     3181 taatacatat gcttgaatct atgggtattc acgaacacta ccaaaatgag gattctttgg
     3241 aatcaagtga tcgcctagaa aacattgagc aatttgtaaa cgcaatcaag gaatatgaag
     3301 aaaacgtaga gaatccaagt ctttccgaat acttaaacga tatagtttta cttacaagtg
     3361 aagaagaaaa aaaagatcta tctgattatg taacacttat gacagtccat aactcaaaag
     3421 gacttgaatt tgattatgtt tttttaacag ggatggagga aggaaccttc ccgcaccgaa
     3481 tgtcaatgga gtcagaaagt gaaatagaag aagaaagaag actttgttat gttgcaatta
     3541 ctcgagctaa agtaaaatta tttatcagtc atgccagttt tactcgtaaa tttggaatgg
     3601 ttgaagatag aatcccatct aggttcttaa aagagatacc tgttgagttg tttgaaaata
     3661 gtaaaactac agaggaattc aaatacagtc ctgattattc accaagagcg ggcgatagaa
     3721 aatcaaatca agatttagat actaaacaga gaagtatatc tactaacggt aattctttta
     3781 gagttgggga tagagttaaa cacaaagatt atggagttgg aaagattatg gatatctccg
     3841 gatcaggtga taacttgaaa gttaaaattg cctttggata cgtgcataaa aactttttac
     3901 ttgcctacac aaaacttgaa aaaataacct agattttatt taaacatttg tatttttaaa
     3961 atctttctct aaaacgacaa gaaatagcat atttctataa gcaggtaacc tatgggaaaa
     4021 ttagattcaa ttctcaatga agaaaaaacc tttgcagcag attgcactct gatcagcaaa
     4081 acaaacttaa aaggtgtaat cacaagcgcg aatcaaaatt tttctaatgt aagtgaattt
     4141 ccacttgagg aactcattgg acaaccgcac aacatagtca gacacccaga cactcctgca
     4201 atacttttca aagatttatg gaataaactt aaagctggaa ttgcctggaa tggtattata
     4261 aaaaatagaa caaaaacagg aaaatactat tgggtagacg ctctcgtatc cccggagttt
     4321 gataaaaatg gcgaaatagt tggttatatt tccgttaggc gaaaacctac aagagaacaa
     4381 attgaagctg cagaaatctt atatcgagat ttaaattcag ggaacactag ttttcgaaag
     4441 agtttagcga gccaattttc ttttattcgt agattgcgaa taggaactcg actctcccta
     4501 ctactagtaa ttataacatt ttcactcatt gctccatccg cagtattaat taaattaaaa
     4561 atggatgaca ttgatctcag caaaaaagaa ttagtgggtt ctaaaattaa tgtatctatc
     4621 tctgaattta ttagaactct aagtaatcac agagggcttt acaatcgctt tttatcatct
     4681 aaagatgaat cgcttttaaa cgatatcgaa ttaaaagaga aagagttaga cggaataagc
     4741 aaagatcttt taagcttact agagactgaa aaatccaatg ggacaactag agaagatatt
     4801 caatctatta ctaaaaagtg gcaagaactg aaaataaaaa atgctcaact agatgttact
     4861 caaaattatg aagaacacgt tagtttaatt aaccaaacac taaccataat gcaaaagatt
     4921 acagaaggat atgagttatt gtatgatccc tatcaggata catttcactt tattaatctt
     4981 tccaatacca aaataattca aatagcagag atacttggta aaattagaaa tgaaggagtt
     5041 cttgtattaa attcaaaaaa caaaacaaag gaatccctta ctattcttga aatgagaatg
     5101 actgaatccc aaacatttct caatgggatc gagggcgata ttcagtatat ttttaaatac
     5161 aatcctagct taaccgacaa atacactcga ctaagagata aaagcgattc cagtattact
     5221 aatttatttc aagttgtaaa agaggaaatc atcaataaag aatatctgac ttatactcca
     5281 caagaatatt ataatattgc aactagcgca ctagaatcta ttcatgatct aaatcgagaa
     5341 ctaaataccg atctacaaaa caagttaaat tcaagaattg attcccaagt tcatgatatt
     5401 tactttacaa ttctaagttt atttattcta cttattattt ctatttctct aacagtgatt
     5461 atatctcaaa gcataaataa accattgcag aatatagtaa aaaaagtaaa gctcatggtt
     5521 cgaggcggtg gcgattttac aaccaaattt gatatgaatg tagaggatga aataggagaa
     5581 cttgcgggat ggctcaatat ttttgttctc cgtataacag agattatcta tcaaattcgt
     5641 agagaatctt caaatcttga aagccaaact aaaactgctt ttaatatagc ggataacttt
     5701 tccaaggctg taagtgaaca ggctaatttt gcagaagaag cctcctcctc agcatcactg
     5761 atgcgcaatg caattagtaa tatttttgag aatgtaaaag aagaagcaaa acatatagaa
     5821 gaaataaatg gcttcatgga tgaattccat gctcaaattc gaaatacaca gttttcaata
     5881 gagcatctac aaaaagtagc tcagaagtta tccgaaatgg caaaattaag ttcagatttg
     5941 attgaagaaa caaacctttc aatcaatcaa gttaacgaaa aatcagcttc aatcgataga
     6001 attgtagatg tgatcatgca aatatcaaat aaaacaagtc tactagcact caatgcctct
     6061 atagaagctg ctagagctgg tgaaatggga aaaggattct ctgtagtagc acaagaaatt
     6121 tctactcttg cagaacaaac tgccaaaaac acaaagagta ttcgagaaat aacacaaggc
     6181 acaaaggaat ctatacgaaa gagtgtagac caaacaagca agacaaatga aaacttaaaa
     6241 tccctactag taaatattgc agacatacat gttaaatctt ttgagcttac tagttcgcag
     6301 aaaaaacaaa aagaaagttc tgaactcatt gttaaaagtt tacatgaaat agaagaaact
     6361 tctaaggaaa ttgtatcctc tgcaaatgag caaaagtctg catcacaagc gatagcagat
     6421 gtattagaaa tgatttccaa taatacagta aaatcatcag agaactcagc atccttatta
     6481 gaagttgcaa agcagacaaa tgctgtcagt ggggatttaa ataaaatgat agagcaattt
     6541 aaatactaat cactgagatg atacaaattt aaaatttgtt aatgaagaag aaagaattaa
     6601 aggatattag agaattctaa tatcctttaa ttcaaaagcc tacgagctcg cctacaggaa
     6661 tctcaatcga tagttgtaaa gatattagga tacttccatg aagattttaa caaaacaaaa
     6721 atactttaga tttattttta tatcctttct tctagtggct aataacacat tcgctactaa
     6781 tccttgtttc caagtgaatg caaaaaatgg attaaatcta agagagccaa gggaaatcca
     6841 attcttccct actgttattg ctagacggta tgcgcattca attattagaa tattcaacga
     6901 ttgacgagca acaaggacga taggcaaaag taaaattgct aaatagagaa cgatggatat
     6961 ttgatgctta tatcaaaaaa aacagaaaag aatgatttac tatcaaatta cttagaatac
     7021 ttagaaaacc ttccttcaga tactagaccc tgaagtgtaa taaagctatc aaaacttttt
     7081 aaaacaaaat aagacttctc gctattttcc acttgttgaa aataattacc aagaatataa
     7141 gatgaatgat tttaaatacg caaatgatat atataaattt aatatcaatc tgttagaatc
     7201 aaaaaataaa tacactaaat atctaattta taaaaatctt ccattattct acttatatcc
     7261 ctaaaattag aggggtaaat atatcttatc cgcaaagatg tatcaattaa aaaaatatgt
     7321 ccagcatgat tgatttcatc tgagccaatt tctttatatg gaaaaatttc ataactgttt
     7381 gtcacatctt ctatttgctt ttgatttcca gtcagtagaa aaaatttatc accgaagtat
     7441 ttagaaaatt cataggaatt ttgtttatct cttttaggat caatagatat aaaaataaaa
     7501 atgacatttt cctcttttaa tctttttgaa agagataaaa atgctcctat agagggaggg
     7561 caggcagttt tacattgaat atatccaaaa aataaaaaga ttttatttcc caaaaaatct
     7621 tttaaagacc ttttagtatt ggtctgatcc tctaaaaaaa aatcttttgc ctctcttgaa
     7681 acagaatacc catacgtctg gcttcgctca ttccaaaaca gatacgccaa taaacaaata
     7741 agaataaaaa attgaaataa aatgaaagtc tttaaatgta ttttagaaat atcaatgctc
     7801 caaattttta atttcctgtt ttaacttttc aactgttgat tccgcctcat tcgccgaatt
     7861 taatatttcc gctgataatt tgttaaagcc tagtgtattt tcattgatca tttcaacagc
     7921 cattaagatt tcctgcatag agtttttttg ctcatagaca gtattcgaaa tctgatcccc
     7981 actctctcta agaaattgaa tttctgaatt caactgagaa aaacttttaa tttgatcagt
     8041 aactgttcca gaaaattgat ttactacttt ttgaatttca ccaaaccttg agataatttt
     8101 tccaaaacta tctgcaccag tctttattgt ctggttggaa ttttttaact ccatttttac
     8161 tattttcatt aaagaatcac tctcacgaat gctgttagat gtctgttctg caagttttga
     8221 tacttctctt gcgacgatgg caaatccctt tccatgctct cccgcacgag ctgcttctat
     8281 agatgcgtta agcgataaca aattgacttg gtctgcaatt tcatagatcc cagatataat
     8341 tgtttgcatt ttctcatagg ctttatccat tttggaaaga gagtttagca tttcgctcaa
     8401 ggtagctttt tccttttctg actccatttg agttttctct acaatcgaat ttgaatgatc
     8461 tagctggttt aaaacagatc tgtttaaatc agaaagacta ttcattaaat cagtaaaact
     8521 cgtaattttc tggctttgtt ttttaacatt atctaaaatc gattccatcg tagaaacaat
     8581 ttctctcatt gcatcgctaa tatcatggat tgaactagac tgttcattgg aagcagtttg
     8641 aatggtgctt gaattattat ttaaagaaga gactatttct ttaatacgaa tcgaaatatc
     8701 atctagattc cgagtagtta ttttattttg ttctaagatt ctacctagat cagctaagtt
     8761 ttcgatttcc ataaaacgtt tttttctagt aatggaaaaa taaatcagca aagaagattg
     8821 gagcaagacg aagcaagcat gtaaaaatac aattccccaa ccatgaccgt aattatatgc
     8881 tatgagagga attcctccga ttgtaacatt attctcttgc agtagattaa aacctatatg
     8941 gtgcaaagca atggttaaag cagccgttat aatcgtcgta taatccatat acatcttaaa
     9001 gaaagcaagg gctgcaaata catgaaagtg catttctatc ctgccaagtt gtgcttcaat
     9061 caaaatccct gaaaacaaca ttacactcag agcaaatacg ttacgagata tcgtagttcc
     9121 tctaaaaaag aaaaatgcaa ctaagcttat aaaaaataca attaaggaac aaactaaagt
     9181 aatataaata gttccatatc ctatagaaac tagtaaagaa actgggacat gcatccctat
     9241 aaacaatata aaaaataaat ctaacttata acattcctct ttatattttt cttctgctga
     9301 tctataatca acattcataa ctatcccctt atactagaat gttcttgatt tgataaaaat
     9361 taaatacact tgcatattag acacaaatat agaaaaataa ttatttcgat ctcttggatt
     9421 gatgtatcca aatataattc tcaatcttga taaagatata aattggaatg atttgtaaat
     9481 aaaattcaaa ttagctttca ataggaaaga tgtctatttt ttctctagta taaagaataa
     9541 agcaatcagt ttaaaaacat aacagaacgt tatttgctat attaaaaatt aagcaaacta
     9601 acttttctgt aaaagtttag atttaactct gaattattca agtttttaga cgtttttttt
     9661 aatttaatat ataaggattg atttgacaat agactttggc ttgttgttat gaattcaggt
     9721 gtatgcaaaa tataaataaa ttcatttggg taaattcctt ttttttctcc ctaaaaagaa
     9781 gtctcttttt tattccttta atacttactt ttgtccattt ttctaattgt gttcgattta
     9841 aaatagacaa cctaaaacca aatctaatca gcaagattaa aatcggaact gacctaacag
     9901 aagtagaagg tatggtagta aataatgcac ttatcaatat tccttttcga attcctatta
     9961 atgcgaatcg actctatatt acagactaca accattctta tatcaaggta tatggaaatg
    10021 atggagactt agactttatc attggaaatg ttcccagcaa gaacaattta aaagttaagg
    10081 tattaaatca aaaattagga aaaataggtt tagtcgcagt aagtagtgaa gatgaccttt
    10141 ttattcaaaa tagaatttct acagacagtg gagctgagga aataaaagca gaaaatagtt
    10201 ttgcaaaaaa ttccggatat tttgatacaa aagaaaaaga aaatacagct tcttatattc
    10261 tacatttaga tagtaagggt aaaatggttt ccatcattgg gatatcagga aaaaactctg
    10321 aaccatttcg gtatatcgaa agtatgtatg catacgataa aaaaagattt gctgtgtatc
    10381 ataaatttgc agaacaaatg catctcaatt attatgttga ggataattta gttggaacca
    10441 tcaaagaagg aaatctaaat atttttactg gaccggattc aaatgaatac aaaattaaat
    10501 tagattctat gataccacat agaagtgggg aatttgccct tgtctcattt agttttattg
    10561 gtaaaaagga cttgcgattt aaatttagac gaatctataa atatgcattt ggttccgttg
    10621 aacctgaaat ccttttaaaa gaatttcaat atccttcaga aattcttttc tccgtaaata
    10681 caaataaaga attttatatt tgggaaacag aaaacaaagg tgaaagcgtt aagctccaag
    10741 tccatgataa agacggaaac catattaaca ataaacgatt gcagtttccc gcaccacgat
    10801 taaattggag agaaacgtat gtggatgaaa atgataatct ctattcagtt aagacaagcg
    10861 gtggatattt ggaattatat tcatggtatt aaggtaaatt atgggaaata aatatcattc
    10921 gagatcacca caagaactaa gagagtatct agacagtttg aatccgaaac caaggaagag
    10981 aacattgttt caatacgtag tcgtaggaaa tatttttgcc ctactctttg tattatttgt
    11041 ttattctaaa gaaaatggct tcttgaatac aagcttcaaa ccatctaata aaatccaact
    11101 agaagaacta gaagcgtatt ttgttaggtc caatgaaagc acgcaggaag agaccagctt
    11161 tttctttttt ttaaaaaatc aatctcaaaa agaaacgtca tttcctaaat cggagatgca
    11221 agttattttt acactcacta cagaagaaaa agaagaatgt cttcaaaaga agtttgaact
    11281 aaacccaaaa aaaattcttc ctaagaatac agaatttttt cctcttttaa ttgaagaaaa
    11341 agaaatcaag aaaacgaaaa catgtgatac attctttcat aaaatccaaa agaaaaatct
    11401 aacagattta ttcgcaaaag gaaatagtaa atttactcct gaattaatgg ttatttatca
    11461 gggacaaatc tataggatga gtataaacaa ttgaaactaa gatcaaaaaa acacccaggg
    11521 caaacatggt ttgccacact cagtatatat ttctttggaa gtgtaatcct tcgtctcttt
    11581 tataaaatca aaattgaagg cggagaaaat attccaaaag agggagcttt aattgtatta
    11641 gccaaacacc aacgattaaa tgacatacca ctcggttgtg ctgcttttta tgtaaaggca
    11701 agaagaaggc tttggtgtat catgaaagat agccttagca atccttttct aggtggatat
    11761 cttttaaaat gtggaggaat cccaattgac cgaaagaatc cagaaaagac aaaggaaagt
    11821 tttaccttcg ccaaacatgt attacaccac aaccaagttc taacactttt tccagaacaa
    11881 acttttttcc cgtggaaaat gggtcgcgga aaagttccgg gctttcgagt gcttgcagga
    11941 aaaccagctc atccacttca agtcatctgt gtaggctttg aatatggaaa agagaaatgg
    12001 ggaagaacga gtgttacgat tcgaattggc aaagtaagtt attttacaaa ggaagactca
    12061 ccagagcttt tcttacatga aaaaatgaaa gaaatcgcta gtttatcgaa tctagaatac
    12121 aactttccgg tgagcactgc aaaagaagaa agtgaagagt aaacgttagt taaatgtaaa
    12181 taaaatttta ccacggctga acacggatgg atttaaaatc accaccgaga gcaccgataa
    12241 cagaccgatg cagaaaagat ggttcataaa gaactatcgt attttttatc ggtgttagca
    12301 gcgcaagcga agcgatcgat gcgaatcggc ggtaatcttt tatactactc agtttcgatt
    12361 tgttgtgcta agtagtttgc gactccgatc tctgcaataa tggattgttg tgcttcgatc
    12421 cagtcaatat gttcttcttc ggcgactaaa atcttttcca tgagttcacg agatccgttg
    12481 tccttgagac gagttgcaat ctcgattcct cgattgagtc tttctacagc agcatattcc
    12541 aaagcaagat catgttttag aatttcttca atattagaac caaccatgat tttcatatat
    12601 ctttgaacgt caggcgttcc atctaaaaaa agaattcttt cgatgacagc atctgcatgt
    12661 ttcatttcat caatagactc atgctgcatg tatttggcta gttttgtgta acatagtaat
    12721 atattactca tgccagtctc cctttgtgac tcatgctgca tgtatttggc tagttttgtg
    12781 taacccttgt ttttattaat ttttgcgtgg ataaaatatt gattaattgc cgttagttct
    12841 gcggctaata cttctcctaa aatctctatg acttctgcat tccctttcat aatttctact
    12901 cctagtctta tatttgtatt tttaaaacag catctcgctt tgaatctttg aattcaatgc
    12961 tccaatcaag catttccatt tcttcaaaaa tgagagaaac tatttatttg aatgaaaata
    13021 tttcttagtg cataattcaa tttttttatt tttatttatt agaataattt caaatttagt
    13081 aattttgatt cctattttca atttttgaat aaattcagtt ttatataatt tacaaagaaa
    13141 aggttttttc attcagtgag ttaaagagcg ttgtatgagt aatatttaca ttcattcccc
    13201 taatctgagt aaattcggaa aacaagactt atcggtttta gaactttcta gacaaacggg
    13261 aaattcttct ctatcccaat ttgatcgaaa tcgcattgac ttgattttat ttacctcctt
    13321 cactccggaa aaatacaata acgagtttca tttggcggca aagctagcag attcactgaa
    13381 tataaaaacc gcatttacca tacgcacaga gactgcctct tctagtggag cttctgctct
    13441 gcaggtaggc tactctatgt tatcctctgg attatttaaa aatgcactta ttgtcggaac
    13501 agagattatg agtagacttg gaagagaaga aagtaattta gttttgggtt ctgttctttc
    13561 tgatcgaatg agaaatttct caatgtcgat ggcacaaggt gcggccatga ttacaagaag
    13621 atatttaaaa gattacgact atactgagtc tgacttattt tatatcgcag aaaagctaca
    13681 tgcaaacgga tcagaaaatc caaacgctca tattcgaaaa aaaataacaa gagaagatta
    13741 cgaaaaagct cctttatttg cgagcccact tggactctat gatatatcac ccctatcgga
    13801 tggatctgcc tctctcattc tatcaacgga aataaagtct gatattagaa ttcgcggtat
    13861 aggacatgga ctgtcgggat ttaaaaccat ggaactgaca acaagttttc ccgcttccct
    13921 agctgccttt tcaaaagctt attcaatggc aaagataaaa ccagaagata taaaaatagc
    13981 agaattacat gatgcattta ctacttttga agtcattgga gcagaagatg ctggactttt
    14041 tccaaaaggt catgcattaa acaaagttaa agaaggagtc agttcgatta acggcgattt
    14101 gccaatcaat ccgtcaggtg gacttaagtc aaggggacat cccattggag catctggtct
    14161 tgcacaaatt gcagagctca ttcgatttat gcgaatccaa aataaatcac tcggactggc
    14221 tcattctatt ggtgggcttg ctacgaataa ttttgcgaca atactggaaa gaatatgaag
    14281 atactaatta tacaaactgc atttttagga gatttaatcc tcactacttc gttttttaga
    14341 gaggtaaaac aaaaatacaa agaggcagaa attcatgtca tagtaaacaa aggcacagaa
    14401 gatattttat ttggaaaccc caatataaca aaggtaatct ccttcgataa agcaaaagtt
    14461 agaaaatcta ttttttattt cttctcattt atccaatgtc tcagaaaaga aaaatatgaa
    14521 atctgtattt gtcctcattt ctcctatcgc tctactctga tttctttttt ctcaggggca
    14581 aaggtgagaa tcggttacaa agaatcagga ttttcttttc ttcatacaaa aaaaatatct
    14641 cgtcctttag aaggaaaaca tgaagttgat aaacttttct ccttaattca tgagaataca
    14701 gatagctttt ctcttaaaca aaaaagacca gaattgtttt tctcagaagc tcgtttgata
    14761 aaaataaaaa ctatattaga aacatatcat ctaaacaaaa attttatcat attagcacct
    14821 tcttctatct gggaaacaaa aagaatgcct acctctaaat ttcaggagct ttttcaaaga
    14881 attgttgcag acactgatta ttctatagtc ttgattggtt cacctaaaga tcaaattcta
    14941 tgtgaaaaca taatcgatag acggcttcct gagaaccaag taaataaggt agagagaatt
    15001 tttaacttag caggaaagac tgatttagta gaactatctt atgtaatcag ccaagcactc
    15061 gccattgtat caaacgactc ttctcctatt cactttgcct ctgctcataa tattccaaca
    15121 attatgatct atggagcaac cgttccagcc tttggttatt ctacacttgt ggataaacag
    15181 tatttatctc aagtagatgg actttcctgc agaccgtgtg gcattcacgg aggtaaagtc
    15241 tgtcccgaaa aacacttcaa atgtatggaa gaacaaaata tagatattat gttcaaacaa
    15301 ttgattcaat tgatcaaata aatgataaaa caatttttta aaattgcatt tgaaaaaaaa
    15361 tacgaatgag accattcaaa tttgcactcg tcgtagaaac tcttttaggt ttcattaatc
    15421 tttttgatcg gataatcact cttagcctaa gtgtaatgaa aatatttaac aatttaatca
    15481 cttatacagt gccctataga gtcttatctt agagtcaagt catgcaaaga ttcgatagca
    15541 ttgaaaataa ataatcttct tgacctattt ttatataagg ttgaatttta aaagttatcc
    15601 taaataatgt gaattcgatg taagaatttt tcaccactta tgaacacaaa tcgcttcact
    15661 cagactgatg attttaaatt tattcctatt ctatcggtgc cttctgtgtt cacaaattcg
    15721 acagtctcag tgcaagtcag tggtaatttt ttaaatcgtt tgttacatcg aaatgacgtt
    15781 aaattaaaag aggtgataaa tttgtttttc aaaaatcatt tactccatat cctatcattc
    15841 ttattcctac tcattcaatg ttcttctctc gaacaagagc gattggcatt tgaaaaagat
    15901 aaattagaat ttgagaaaaa gaaattacag tttgaaaggg aaaaaattaa atcctgtgga
    15961 tgttgtagcc cttgtaatac ttgcaacggc actgcagaag ttgtagtagc agaaaaaaaa
    16021 gaaaaagact gtatcaaaga agttccgcct acaagcgaag atatttgggc tttatccgtt
    16081 gaaggtaatt ggcattatgc tccagataaa ataaaaaata aaaatatatg caaaggagat
    16141 ttaaaagaaa ccttttataa gcaatttgca aatacagaaa gctatgtagc agaaaaacaa
    16201 gttccagcaa caaaacaaag tagctgtatc aataacgttc ttttttcagg gaaatcacag
    16261 ctctataatt taatggttga taagacagcg aaagaagaat ctatttctat ttccaaagag
    16321 ttaaaggcac aagtattaca agaatctatc gaatacaata atgctgaaaa aggtagaagt
    16381 ttctattatg aatgcaagcc aacagatcca aatggaagat gggatagttg taaatgtgtc
    16441 ctatatgttt cctatccaga gggtaaacaa ggattatccg ctcgacttaa aaaataaaag
    16501 gaactcaaac tatgaaatat ttatcggttc taatcatatt ctcactagtc tactgctctg
    16561 cctttggtaa agacccagaa ggaactcacc tcgaaaaaat aaaacaatct ccccaatttg
    16621 ataaagagag agatcaattt gtaaatagaa gagcggacat tctagagaaa atgagagaga
    16681 atcaaaactt tttcaaatta actcttttat ttttttttgg cggagaaaag catcaaagac
    16741 cacccgaaaa acttcctgag ctaaaaccag atgtaaaaga atttttagaa aagtcagaca
    16801 aaataaaatt tatttggttt ggtcattcta cattcttagt gaatttcgat ggaaagatca
    16861 ttctttttga tccagttttt tcgaataacg cttccccgat tagccttttt gtgaaaagat
    16921 ttcaacctcc tgttttaaaa ttagaagaat tacctaatat cgactatatc attatatcgc
    16981 atgatcatta tgatcatcta gacatggaga cgattgaatt ttttaaatcc aaatcagtca
    17041 aatttataac acctttagga gtaacgtccc atctaaaaga atggggaatt acagaagaca
    17101 gactcacaga actcgattgg tggagttcaa tcaatcgaga cgatctagaa attattctaa
    17161 ctcccgctca gcatttttcc ggaagaaaag gaaacaatca aaataagact ctctggggat
    17221 cttggattgt cagaaataaa aaacaaagtc tttattttag tggagattcc ggttatgata
    17281 ctcacttcaa agcaataggc gataagtatg ggccgtttga tattacattc attgagaatg
    17341 gacaatacaa cgaaagatgg cgagcagttc acgttctacc cgaagaaaca gcccaagcct
    17401 ttttggatct aaaaggaaaa catcttgtcc ccgttcattg gggaatgttt gatctttcat
    17461 tgcacaattg gtatgatccg attgaaagaa ttgaaaaagc atccaaagaa aaaaacataa
    17521 aactactgac tccaaaattc ggacagattg taaaactaga agaagataca attttcgaaa
    17581 catggtggaa gaagttcata caagaataaa ttgtaactac tcagcccgaa aagaagatta
    17641 ccaccgataa aagagacgat aattcgaaat tttcaatcat atcttcatcg ttcagttatc
    17701 ggtgttcatc ggtggtgaaa aaaattttac tccgaattca agttaaaata ctgtccgatg
    17761 aatatagcag ttaagaataa atatttactt ctatagtcta cgtataaatt ggatttatgg
    17821 cgaatgaaat ctataaaaaa cgaatcaaaa aagttcaatc cattttaaaa gagggagaga
    17881 ccttactcct atttgcggca aatcatctaa ttcgaaatag agatgtagag tataaattta
    17941 gacaagactc tgactattat tatttaactg gaattaacga acccgatgga attctagttc
    18001 ttaaaaaaaa ctatagcggc ttatttgtat tacccaaaga caaagaaaaa gaaatctgga
    18061 caggaattcg cttagggaaa aaagaagcaa agaaactttt atccctagat gaaacatttg
    18121 atacaaccga gtggaatgat aaatcgatag aactactcac aaattcagat actctctttt
    18181 atttctttgg aaaagacaaa gacagagatt ctggactgat tgctttatgt gatcatttaa
    18241 ataaaagatc taggatcgga caatatggtc cttctaaaat tcaaattcca gaattcttac
    18301 atgagatgag aatgatcaaa tcaaaagaag aaatggatgc aatccttgaa tctgcaaaga
    18361 tcaccgaaaa aggtcatatt gcactcatgc aggaaacgaa acccaatatg tatgagtatg
    18421 aactagaagc aattttagaa agggaatatc tcagacatgg agcttggggg ggcggctatg
    18481 gacatatcgt tgctgccggt ggaaatgcta ccatcctcca ctacactttc aatagtgcaa
    18541 aaattaaaaa gggagattta atcttagtag acagtggtgc ggaaaagaac tgttatacgg
    18601 cggatgtcac aagagtctac ccggcagata agaaatttac ctctcctcaa aaagatgtgt
    18661 atgaaattgt tttagacgca caaaaaaaag ccattcggaa tacagtcgct ggcaaacagt
    18721 ttttagccat tcataatgaa acggtcagag atctaatcgt aggtctaaaa tccttaaaac
    18781 tattaagagg ctccgttgat aaaatattcg aaactggtga atatagaaga ttttatatgc
    18841 ataagactgg acactttctt ggaatggatg tccatgatgt tgggcgttac ttcgaaaaag
    18901 gagaaagccg taaattagtc aatggaatgg tgactaccgt tgaacctgga ctttacttcg
    18961 atccaaccga taaaacgatt ccaaaacatt tcagaggaat tggcattcgc atcgaagatg
    19021 atattttagt gcaggggaaa acaccgatta acctcacagc atcgattccg aaagaagttt
    19081 cagaaatcga agcaattcga gaaactgtag attagagaat aaaacggtag attagaaaac
    19141 gttttctagc ctaccgttgt caaatatcat ccgaaaagaa aattgtgtaa tctgaaacga
    19201 tttttctttt cagatttaag attttattac aatcttaaat cttttccatc ttgatcggta
    19261 gaaatacttc tgcaagaacg caattagttt ttcgttttcc ggtatggtca attgcattat
    19321 gaataaaaaa cattccattg tgtttattta caatgaagtc tacaacggtc aatcctagac
    19381 ctaaactaaa tttttctact tctaggatat cttccacagg tggatgcaat cgaaaaaatg
    19441 gctctaaaac taatttttcc ttttccggcg gaattccgcc atacgatttt tcatctatct
    19501 catttttaaa cgttatgcaa aaatacgaac ctgcaacatg agcaaatata tctacacgac
    19561 tggaaggtgt cgaatattta tatgagttaa tcagcaattc ctcaaaaact aactttatct
    19621 tttctatatc aatttctaat ttgcttttgc tggttatatt cggaaaagct accctcgtgt
    19681 ttttattttt taaataagga aggatatgac tcaaataagt tggtaattct cctgcgagtt
    19741 taaaagcttc catttctttg aaatcaaatg gctgttccat taagtctaca acttgtccta
    19801 aaccaaataa tactcggcga ctgtattcat tattttcaaa aacagtatct agaatttctt
    19861 tattcacaac atacgtatca ccttgctcaa tcatatccaa ttttaacata tctaatagag
    19921 atatatttac tccaacccct cctccttgag aaagagaggt tgataaattt acaattgagt
    19981 ttttttgata tgaatctttc ccgctcactc gaagtgtttc tttataattt agccattcta
    20041 attgacctct taattttaaa ctttcattta aacttaattc tgcctcaatg tcttttagat
    20101 atttatattc gagtgctttt gttaaagtat gttgaaaaaa ctctatatct atcggtttaa
    20161 taatataatc gaaaacacct aatctcatca cattaataat tgtttccgac gcatcaaggg
    20221 cagactgaat tataattact gcctgtggct caatgatttt taattcttca ataaaggatt
    20281 ttccatctag gattggcagc atcaagtcaa ctataaacaa agaaaatttt cttgatttag
    20341 ataagtctag agccatacga ccattatccg caatcaccgt agaaattcct agttggtgac
    20401 aaatactttg tatcaatact tgattttctt ttttgtcttc aacaattaaa acaggatatc
    20461 taggaatttt tatattagtc ataaaatgag aaaccccttt taatttagat aatatttgta
    20521 gaaataagtt ttttatgaat acttagcttg ataaaaaaaa ttgagaaatg aattccataa
    20581 agtgtttggt ctattttgaa tgtcaataga aaattgaatt gtttattatt tatatgttat
    20641 tttattcatt aaaaacaaat tcgattcaaa tagaattatt ttgtaagtcg agttaggtaa
    20701 aaactcttaa ttcgattcta tgcaaatact tagatgtaaa aatatctaga aagcatttct
    20761 gttataatga tacgatagtt tcatcataac actaaataaa tattttatta tcctatttta
    20821 taaaccagaa agatttcttt ttagaaaaaa ttgttctcct agattttttt ttaatttgta
    20881 tttaattatc tttatttagt tagcaggcaa attcttacaa ggtagctctc gtataaaacc
    20941 cgggataaat tgcacttgac ataaaacgtc gttttgaaaa cctatttagt cttttgttat
    21001 gagtgcaaaa gttatggaac aaggtttttt aaatctagag tatcaaaaag aatttggcaa
    21061 ttaccgatta aaattactcc tagcgactga tgaaattggt attatatata gggttacatc
    21121 cgtattatat gcaaatgaat ggaatatctt aggtgccaat attagaagtt tagaaaatgg
    21181 acagattgag gatgaattct taatccatca ttccaatgga attaatttag atgatgaaga
    21241 attaaataaa ctgaaatctg aaatggagca attgtttaga gataaaatct caattacctc
    21301 ctacatgatt aaaaaaggaa aaaaaatcaa taatcaaaaa ggaaatgagc ctgctaaaat
    21361 aagttttgaa actgtggcta acaaggattt aactaaacta agaattcaga caaaagatcg
    21421 gtcaggactc ctttgtgata tatctagaat gctttttcta gaatgtatgg atatattatc
    21481 acttcaagca aagactcgtg gtgatgaagt agatgataca tttgaaatca agtcagaagc
    21541 aggacatagt ttagacgaaa caatgcaagc aaggcttaca agaaacctgc agaatgttat
    21601 atagaactaa gattatctat atattttccc aaaacgccaa aagcaaggat tagaaaaaaa
    21661 atacaaaata ttaaaataga aataggaata gaaggactca atcgaatcaa aactaaggag
    21721 ctaatcacaa ttagcccaaa gaagagaaaa atgaatatct tattctttgg gttcatattt
    21781 tgtttaaact gatttctaga atgaatggat aaatctcttg ttttcgtttc aggattgtag
    21841 ccaggatagg aaaataaata tagcaaagct tccttgattg aatttgcatg aatcagattg
    21901 gaaaaaactt ctttatacaa atgaaagttt gcccaaaggg gattataact attaatcggt
    21961 tttgttatcc cataaacagg ttcctctact tcctcctgaa aagttcccaa aattcgatcc
    22021 caaacaataa atacaccagc atagttttta tcaatataat ctgggtttac tgcatgatga
    22081 attctgtgat gggaaggcgt tacaaaaaca aactcaatcc atcttggaaa tttttgaatc
    22141 aatcgggtat gagttccaaa ttagtaggtt cgattcaggg catcgcaaat tagaatcacc
    22201 tcgtaggaaa tattcagaaa aacaaggggg aatacaaaaa agaaataaaa aacatttctc
    22261 aacattgtct gcctaagagc aacacttaag ttaaactctt ctgaagaatg atgcacaacg
    22321 tgggcagccc ataaaaaatt tatctcatga gaaaatctat gaaacgtata ataaactaaa
    22381 tcgatcagaa taaaaacaag aaaccaagaa cagaatgcaa aggcatgaat tcctacaaat
    22441 ggaaaagaat tatcaattgt aaatggattg gaaaaatcat aacttaggtt ccgtattccg
    22501 acatagtctc ggattgcttc aaaaataagg aaggaagcac ttaaaaccaa tacatcgcct
    22561 aaacgactca tggctccggt ggaaatatta gctatggaat caaataagga ataaactttt
    22621 ttctgagtaa aataagaata agtaacttca attagaatga aagcaatata gaaaggagca
    22681 attttttcaa ttagttgcat actacaaatt cttctcgaaa tctttaatcc tatttacaaa
    22741 gaaaaactat tttcactgca aaattagaaa attcaatgaa gtatttcgtc gatgcaagct
    22801 tcaaaaaatg gaaaggtata tggcattttg gaattgattc tttcctttta caaaatgcca
    22861 caagggttga aacctaaaaa ttattgcttt gctggtgctg ctggagttgg atttgctggt
    22921 tgtgttgcgt ttgtcggtgc agtttgagaa gcaggtgctt ggtttgtgcc atctgctggt
    22981 tgctttgcat cctcttttgc aggaacaatt tcagacgttg gaagttgttc aataattgct
    23041 tcttctttct ttgcgaataa aaaagagagc aaaagagaaa ccacgataaa cgatatagca
    23101 atgaatcgag ttgctttcgt aagaacatct gcgctagaag agccaaatgc agtctgactt
    23161 g
//
LOCUS       DAOQOS010000089        22710 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_1086_length_22710_cov_5.950475, whole genome shotgun sequence.
ACCESSION   DAOQOS010000089 DAOQOS010000000
VERSION     DAOQOS010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 22710)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22710)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22710
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1086_length_22710_cov_5.950475"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(318..953)
                     /locus_tag="PLF66_17615"
     CDS             complement(318..953)
                     /locus_tag="PLF66_17615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65913.1"
                     /translation="MNMYNNKVEILFHGEGCGEVKGDYLIEESKIRFNLTKPASEGCE
                     NNKVLYEEFKKAICSIYKDYTHPKFYYRFECSNKRIFYGFENPENLEKNINGIDVITL
                     EVKPKFAPKNNIPVYNKPEINSMPDTFNLFDVDYRSRPEVLNLPDDKRFTTNFCESVG
                     NGEFQILSKTKNRYGGIYWYHIIFNLGWYEGGIGYGWIKEEDVGGCVDKFK"
     gene            complement(940..1347)
                     /locus_tag="PLF66_17620"
     CDS             complement(940..1347)
                     /locus_tag="PLF66_17620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65914.1"
                     /translation="MISQSNNYFSVKCKLTTTNVIITLLILSSFLFMCKDKDSSSLKK
                     PDIHIAKEKIILFTIPDFNSPKVKEFEIKKPFKIIEFSTQLAKFENKIAPWVKIQSGD
                     LIGWAFGYYIPIELKEDLLIKNRFDDDEKNEYV"
     gene            complement(1448..1879)
                     /locus_tag="PLF66_17625"
     CDS             complement(1448..1879)
                     /locus_tag="PLF66_17625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004488599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HMZ65915.1"
                     /translation="MKKAIIDTGVIIAFFDESDKYCHAVRSFFKTYKGELYSTESVIT
                     EVSYYFENYKKAQLYIIDWIGKGAIKIVNVNNEDYPLVHSMMTKYQDTPMDYADASLI
                     LIGEKLKITEILTIDNDFSIYKFKNGKQFKNLLQEVLRKKN"
     gene            complement(1876..2130)
                     /locus_tag="PLF66_17630"
     CDS             complement(1876..2130)
                     /locus_tag="PLF66_17630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HMZ65916.1"
                     /translation="MTTLRLNREIEYKLHKVAKIESRTKSDIIKELILNYLDSYEENK
                     KTPYELGTQYFGKVKTGNKNGSVEYKSIISEKIKTKLRKK"
     gene            complement(2218..2385)
                     /locus_tag="PLF66_17635"
     CDS             complement(2218..2385)
                     /locus_tag="PLF66_17635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65917.1"
                     /translation="MKIVFGEPISFDEEYCQKILIENSSDSSNSRESVQFITDWTFAV
                     LKKLEQSYIKD"
     gene            complement(2575..2991)
                     /locus_tag="PLF66_17640"
     CDS             complement(2575..2991)
                     /locus_tag="PLF66_17640"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HMZ65918.1"
                     /translation="MKSFFDTSALVKKYIEEDGSDAVSELFQNSDEIALSPITRIEFQ
                     SALQRLVNTNVLTQESYEIALAEFTEDSVDYEFISFNSTLEELVIEVIKKYGLRSLDG
                     IQLAAAKISKSDQFITSDTKLFEAAKKELPQKNIFI"
     gene            complement(2988..3176)
                     /locus_tag="PLF66_17645"
     CDS             complement(2988..3176)
                     /locus_tag="PLF66_17645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65919.1"
                     /translation="MIILHGFYDNGKVTITDKNIPDMKADVEIIIRKKSWQRNVRRVK
                     LSNKNSASETILQMRNET"
     gene            3668..3877
                     /locus_tag="PLF66_17650"
     CDS             3668..3877
                     /locus_tag="PLF66_17650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65920.1"
                     /translation="MYFDPICGTSGNEWGDLTTFDPIQSRFLTLKTSASIVMSDVVHC
                     KLPTQLVNLEYNWIGFWIDYKKKND"
     gene            3896..5476
                     /locus_tag="PLF66_17655"
     CDS             3896..5476
                     /locus_tag="PLF66_17655"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HMZ65921.1"
                     /translation="MITAIKNAYSEYQKFWIKLFIQKYIPEEQITKTLVEDLNQKIIF
                     HNLYAFRITSVIVVLIELVLIFTFDLPMLKSASAENQFLYLGYLYSHISIIALSVFCN
                     FILYLPISAYKKLCYHHGIIVPLYSFFLLVICGYMDALNYYSTKTMNLFIIVMLMQLV
                     VFIFSMHFILFHLIINFGLLIFFIDYYKSFDAGALNSIINIGILSIFAFVSGYLRFRG
                     FVRNEIIEKQRQALDVQMKLTNSFERFVPKEFLSFLNRTSVLDIELGDQTQREMTVLF
                     SDIRSFTTLSESMSPQENFNFINAYLSRMGPHVRQNQGFIDKYIGDAVMALFPNKPDD
                     AINAGIEMQKEIILYNKNRTEKGLTPISIGIGIHTGNLMLGIIGETERMEGTVISDTV
                     NLASRLEGLTKLYGITIMISEDTLLKVENIEIYKYRLLDNVVVKGKQNSIRVYQIFEG
                     MSEREEELLQATKLSFELGIEAYLGRRFTEALELFQNVANVYPEDVPTQIYLKRCNKS
                     ITEGVSDDWTGIERMTEK"
     gene            complement(5636..6061)
                     /locus_tag="PLF66_17660"
     CDS             complement(5636..6061)
                     /locus_tag="PLF66_17660"
                     /inference="COORDINATES: protein motif:HMM:NF014820.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65922.1"
                     /translation="MQIPRKKSYALERGYTPLAEVVSSAVSGVHPSLLGTGPIPASLK
                     ALHRAGLKRETIDLWEISEAFASMVLTTIKELNLEKEKVNVNGGGISLGHPLGASGAR
                     MALTLSHELIRRNLNYGMVTMCIGVGQGIATILKRYEKR"
     gene            complement(6158..6367)
                     /locus_tag="PLF66_17665"
     CDS             complement(6158..6367)
                     /locus_tag="PLF66_17665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65923.1"
                     /translation="MSQLITLEGDRQTNNYEVLEIHGDFLNQNDLGDYVEGDFFPDKL
                     LVIDKNDLNGDPYLIDISGKKFYPV"
     gene            complement(6524..6970)
                     /locus_tag="PLF66_17670"
     CDS             complement(6524..6970)
                     /locus_tag="PLF66_17670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65924.1"
                     /translation="MKAIEQKLINQDQTVSEIQRIAKQYVSDLDNFKDWDFFKFYNLV
                     RALPYVADPIGKETLSRPCYTILNDWTGSRDCDDKTILLVSKAIQNKIPYRIVICGQG
                     NYAHHVYPEILFCGLWLPADATYPNRSVFGKYLYKEGFRKVFPEKK"
     gene            complement(7067..7492)
                     /locus_tag="PLF66_17675"
     CDS             complement(7067..7492)
                     /locus_tag="PLF66_17675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65925.1"
                     /translation="MNKKNILMFVSIFGFLAIYIFRKKIFGGKIQNNFSKLTIFYKPE
                     EDPTNDPNGKALLLMPNQTPNERVDGITFPSLKNGLVYKIPDGCIVNILENNEIEIVY
                     CPTESISKWNGWQSETFLNYLHSINDNTWNKLFEKSKGL"
     gene            complement(7521..7766)
                     /locus_tag="PLF66_17680"
     CDS             complement(7521..7766)
                     /locus_tag="PLF66_17680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65926.1"
                     /translation="MQENKSLYTTTQNKDILLAILIQQDKEELTENSIVEIFDPIHRD
                     IIERCRWIHAVNKIICDPEIPLMPVILEQEKKLKKIV"
     gene            7808..8062
                     /locus_tag="PLF66_17685"
     CDS             7808..8062
                     /locus_tag="PLF66_17685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65927.1"
                     /translation="MNENDKDIIRVVKIFDMRTGKIKEIIHFQINKKYFLKRKGKIRE
                     CIILEFFSKEKHSKAKIIFSDTQKLGEAFLDELHESPENT"
     gene            complement(8073..8324)
                     /locus_tag="PLF66_17690"
     CDS             complement(8073..8324)
                     /locus_tag="PLF66_17690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65928.1"
                     /translation="MKNIKKKSDNRNTIISNFTSKNDSYKIYLSKYRGKLISFQIYKD
                     LIFHADEKGKLRCIEILKFSLKFPDFNKNKKPRKLKAIN"
     gene            complement(8327..9115)
                     /locus_tag="PLF66_17695"
     CDS             complement(8327..9115)
                     /locus_tag="PLF66_17695"
                     /inference="COORDINATES: protein motif:HMM:NF014537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HMZ65929.1"
                     /translation="MFKNCKWNRTKREGSRPAGITRIDFALDDFEGCIDLDLLEYKIK
                     KGCYVTRLVSSMNTTGFNLRDRVESGKTIYLGDRGAETFFRYYDKKAKCKNDGIEIDP
                     SIDVWNRMEIEMKLGRANWFTYNYLNNENFNPYEAILGMFRLTQKPLSKKQKNKHLFE
                     VDRLYSKFLHNTKKATPLKVPQLVYDLQNLENYVEKTTSGASDTYIRIFGLKKYLEVL
                     ENRKFKTNETPKYSALLEKNSGSFEQEDYTAIYNPKIKTFKKVS"
     gene            9104..9268
                     /locus_tag="PLF66_17700"
     CDS             9104..9268
                     /locus_tag="PLF66_17700"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HMZ65930.1"
                     /translation="MFEHPYYKKNYNFNRKSFPNACRMYDKALSLPLFAGMTDEDVED
                     VIFAVQDILR"
     gene            9620..12490
                     /locus_tag="PLF66_17705"
                     /pseudo
     CDS             9620..12490
                     /locus_tag="PLF66_17705"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
     gene            12548..13303
                     /locus_tag="PLF66_17710"
     CDS             12548..13303
                     /locus_tag="PLF66_17710"
                     /inference="COORDINATES: protein motif:HMM:NF020036.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HMZ65931.1"
                     /translation="MTGNWTFHFVDNSLNWSEELYRIFGFEPFSFTPTFENFISIVDL
                     SDRERVIREFENAKEHKTNYQLSYKIIQPAGEAKYIEEIVEFYSDGSGYLLGCIKDIT
                     QPHSLLQNEKKINEDLQNLSSEVKLENINLNYTNSFLIKSMPFPFVYGKIILDEKGNS
                     VDYEFIEINEAFEKITGLARENVLNKNVKSIYPDIELNWIEKLGKVALSGDSLTFEEY
                     SKITNSTFIAKAFSPKIGYFCSIFTEKEKSSNV"
     gene            13296..15695
                     /locus_tag="PLF66_17715"
     CDS             13296..15695
                     /locus_tag="PLF66_17715"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HMZ65932.1"
                     /translation="MSDFLKELRERAEKVLKKSDIDPESLTKDEILAIIHNLEVHQIE
                     LELQNEELLNAQSALKVVNRQYIDLFELAPVGYIKLSHEFGILEVNHLFCSWFKDTID
                     FHKTSFESFISHTDQDIFFRAKRKIQKTKENQSIEIILRNNLFVKLDIKCYFDDSYFL
                     TLTDISELKEKEKKVLELNNRSMALLEGFPDLVLFINSRGELIESAANQRNNAIRSLN
                     DIFPKDSISIIKEKIQRAVFDGFCIFEFQKTNKVGKIEYLETRLVKSNQDEIIIIIRD
                     LTDKIQANLELEKSKNELELIYNNIPILVSHVDNDFNYIYVNKAYNDFFGIEKIIGKN
                     ILDVIGPESFEKSLPNLNKALNGISVQYENRIYNSQNIPYDVNINYIPNVLKNKVDGI
                     FIIVADITDRKVYERKLLEAKELAEQANKAKSIFLATMSHEIRTPMNAIIGFTDLLNQ
                     TVQDETSRNYVNYISSSGNLLLKIINDILDLSKIESGKMILEPISSDLIEVCKEIIDI
                     FSRNFKNKNLKFIFEKSKDFPKIIYLDELRLRQILFNLIANAEKFTQEGFVAVYLHFN
                     YHQNPNMKLGDLEIRIEDSGIGISSEDLANIFSAFTQAKGQDRGRYGGTGLGLTISKK
                     LIQLMGGEIEVTSELGKGSQFQILLKNLTYEDISHRTENKDSTFDLSPFKNKKFLIAD
                     DVELNLILLKQTLTKFNFQNVYQAENGLEAFEITKKEKPDLIFMDIIMPVMDGIQATQ
                     NIRNFKELENTKIVAFTASVDSISKLESMGLFDLVLLKPIKPKEILEAIYKCFNAEKL
                     S"
     gene            15725..17407
                     /gene="kaiC"
                     /locus_tag="PLF66_17720"
     CDS             15725..17407
                     /gene="kaiC"
                     /locus_tag="PLF66_17720"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF006799.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circadian clock protein KaiC"
                     /protein_id="HMZ65933.1"
                     /translation="MNYFTNQLLKSPTGIEGLDEILEGGIPKGRITLITGDTGTGKSL
                     MALEMIVNGATKFDEPGLYISFENELKEIFQDLHSIEWNLENLSKENKISLKHIDVQV
                     DETESIGSFSFDILLKRIEKQINEIGAKRVVLDTLESIWSVVGVENISRWELRRLFKW
                     FIDKGITLIVTGEKRDGTSHFFFNEFISDCIIQLEQKIFSEIVTRRLRIVKYRGTPHG
                     MNTYPYVITSKGNKVFPITSMNLGHHVSTEKISFGMEEMDKMLGGGIFKGSSVLLSGT
                     SGTGKSTFANFCANHICRNGGKVLYFAFEESAEQILRNMVSLSLDNRKFYDEKQFIIF
                     ARRPNSMGLEQHLLSIFSLVEEHKPELVILDPISNFSLNIHDDNIKSFLMRLVDFFKQ
                     RIITTILTSLIRGSGVLEETDEGVSSIMDTWISLQMVEQNNERNRIIKILKSRGGAHS
                     NQLREYILTSDGVHFEDVYLGAKGMLTGSQRLIEIAKNEADEKLRLDKLQRIRDEILI
                     LENNFEAEKSILYSKFEKEKKELEEELLKGELITKVNKSDKEQLINKRNQGL"
     gene            17413..17787
                     /locus_tag="PLF66_17725"
     CDS             17413..17787
                     /locus_tag="PLF66_17725"
                     /inference="COORDINATES: protein motif:HMM:NF019309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0048511 - rhythmic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="circadian clock KaiB family protein"
                     /protein_id="HMZ65934.1"
                     /translation="MAEISVKIFVTTKDLASDIIQAWKEELNLLKNLSFTFEIIDFDS
                     NPQQIEKYDILFSPTTLITLPEGKNVRFIGYNKNILVYLRAYSLKIQSEIDKTMASSH
                     LDQSLEMKIGIAKMRKFMNPKV"
     gene            18344..19582
                     /locus_tag="PLF66_17730"
     CDS             18344..19582
                     /locus_tag="PLF66_17730"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMZ65935.1"
                     /translation="MMNKVIEVKNLTKSFKLFHSNKDRLINIIPFINKKFDVHTSLNN
                     ISFDVKKGEFVSIIGKNGSGKSTLLKILCGVLQKTSGEYIVNGSIVSLLELGTGFNPE
                     LSGRDNIIVSSKTMNFDINYIYSKMSEIEAFADLEEYFDMPIKTYSSGMYVRLAMSLF
                     LHLNPDIFIIDEALSVGDIFFQQKCFSKIEQMRKNGVAFLFVSHDINTVLNMSDRVLL
                     LDHSKQIYFGDKHEACKLYYDSDKKVLIPENESDEIKEKIEVSIENNILNGKKQIIHR
                     KGATLDSLQIVDEFGNHTMQVEMTKSLKFIIGYKVDEILDNPAIAISFYNNKNMLITT
                     IVYDLVVDKRFIEVAIPFMLEAGEYTFNLTIAQKVKNRGVSHQEYTNVGPISVTFDFN
                     SNEPSFYGLVGLDYKINFKD"
     gene            19591..20424
                     /locus_tag="PLF66_17735"
     CDS             19591..20424
                     /locus_tag="PLF66_17735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65936.1"
                     /translation="MRQYEKNITSQFGEDGIIEYLFSKIGVSNEPWCVEFGAWDGEYL
                     SNTYNLVFNQGFNCVYIEGDSNKAKELRNKVSKLSNQKIFVLNNFVESQGVNSLDNLL
                     GQTDIPKEFDLLSIDIDGIDYHVWKNFKDYNPKIVLIEHNPTIPPHLEYIPDEKSSLV
                     GASTLALYGLGQLKGYELVYCTDTNSIFVRKDFFQLFNINDNTPEKLMKKDHITYLIS
                     KYDGSYLVNKEPAFINIFDNNIFNNAKKDIPIWQKLFLYYKLIKNFRIKGIYIREEKF
                     L"
     gene            20421..20996
                     /locus_tag="PLF66_17740"
     CDS             20421..20996
                     /locus_tag="PLF66_17740"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HMZ65937.1"
                     /translation="MIKNLYIIFIKTIAELLSPLPIVIEQKKWEKRNIALRLLGLNIG
                     KNVRIGPDFLTINGLENNISIGDYTDIGYANKFYSFNKIIIVKFNMFAANIEIVNGGH
                     NVNNLEPFSKPIIIGNGCWFGHGVKIIKGVTIGNNVIIGGGSVVIDDIPDNAIAVGVP
                     AKVIKYRELPDKVWHLGNEYFCPKTFELIKD"
     gene            20998..21309
                     /locus_tag="PLF66_17745"
     CDS             20998..21309
                     /locus_tag="PLF66_17745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65938.1"
                     /translation="MNLYETINNLLKKPVYLIDVGAYKGEFTDNFLSNINKIEFIDMY
                     EPQDSSFVDLKNKFSNNKNINIYNFAIGNENIQKEFFYFENEGYKSSLLKPIEGGVKC
                     L"
     gene            21300..21611
                     /locus_tag="PLF66_17750"
     CDS             21300..21611
                     /locus_tag="PLF66_17750"
                     /inference="COORDINATES: protein motif:HMM:NF016908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HMZ65939.1"
                     /translation="MSIVEIKTLDEIYKKVENAYLKYDYFLKLDTQGNDLSVLKGAKN
                     FIKNSQPIIFCELIYVPLYENQSYAHEIINYMMENDYVLLSRNLHRTSPIIVSHFYLT
                     C"
     gene            complement(21725..22282)
                     /locus_tag="PLF66_17755"
     CDS             complement(21725..22282)
                     /locus_tag="PLF66_17755"
                     /inference="COORDINATES: protein motif:HMM:NF017496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HMZ65940.1"
                     /translation="MSFPVLENPEVRKLFSTVSVEKYMQMIGLGVIPENTELIEGVIV
                     HKMTKGPDHSYFSDILFDALSKIKPMHTIIRSEKPLTLLRTVPEPDISIISGSLEDFR
                     NANPTTALLVVEIAKTSLNYDREKIPIYAEAKIENYWIVNLSKQQIETYQNPNGDDYS
                     NKITYSAQDIISIFGKEIHLKEIFK"
BASE COUNT         8091 a         3163 c         3637 g         7819 t
ORIGIN      
        1 ttgttattat tgttttttta ttaaatttca aattatcaac tctccccaat tttagataat
       61 tttctttact aatttttatt taataaacat agtaagtata ttgtaaaaaa aattttttaa
      121 aataatttta taatcctttt aataaaatta ttatattaat aatgattaaa ttttttattc
      181 tatataaaac taatgattta attagtaaat attaaaacaa aaaaagtgat ttaaaaaggc
      241 ttaaattaat aataaatata aattttaagg ttataaactt atatttaatt aaagttgaat
      301 tctattttaa tgcgattcta tttgaattta tctacacagc cgcccacatc ttcttcttta
      361 atccacccat agccgatgcc accttcatac caacctaaat taaagattat atgataccaa
      421 tagattccac catacctatt ttttgtttta gaaagtattt ggaattcacc attcccaacg
      481 ctttcacaaa aattagttgt aaaacgttta tcatccggaa gattaagaac ttctggtctt
      541 gagcgataat ctacatcaaa taaattgaaa gtatccggca ttgagtttat ttcaggctta
      601 ttgtaaacag gaatattgtt cttaggcgca aacttaggct taacctctaa ggtaattaca
      661 tcaataccgt ttatattttt ttctagattc tcaggatttt caaatccata aaatattctt
      721 ttattagagc attcaaatcg atagtaaaat ttcggatggg tataatcttt gtatatagag
      781 caaatcgctt ttttaaattc ctcatacaat actttattat tttcacaacc ctcactagcc
      841 ggtttggtga gattaaaacg aattttactt tcttcaataa gataatcccc tttaacctct
      901 ccacaccctt ctccatgaaa gaggatttct actttattat tatacatatt catttttttc
      961 atcatcgtca aaacgatttt ttattagcaa atcctccttt aattcgatcg gaatataata
     1021 tccgaatgcc cagcctatta aatctccaga ttgtattttt acccaaggtg cgattttatt
     1081 ttcgaacttc gccaattgag tagaaaattc tattatttta aatggttttt tgatttcgaa
     1141 ttcttttacc ttcggagaat taaaatcggg gatagtaaat aaaatgattt tttcttttgc
     1201 gatatgtatg tcaggctttt ttaaggaaga cgaatcttta tctttacaca tgaaaagaaa
     1261 gctagaaagg ataagaagag ttataatgac attagttgtg gttaatttac attttacgct
     1321 aaaataatta ttactttgac taatcataat attcaagcac tctaatttct ttaatttttc
     1381 gtgatttaga atttggacat ctaggctttg cattaagcct ttgttttgcc catttctaaa
     1441 ctccttatta attctttttt cttaagactt cttgtagtaa atttttgaat tgttttccat
     1501 ttttaaattt gtaaattgaa aaatcattgt cgattgttag aatttctgta atttttaatt
     1561 tttcaccaat aagtattaga ctcgcatcgg cataatccat tggagtgtcc tggtattttg
     1621 tcatcattga atgaactaga ggataatcct cattatttac atttactatt ttaatagctc
     1681 cctttcctat ccaatcaata atgtaaagct gagccttttt ataattttca aaataatacg
     1741 atacttctgt tataactgat tcagtagaat aaagttctcc tttgtatgtt ttaaaaaagc
     1801 tacggacagc atgacaatat ttatctgatt cgtcaaagaa agcaatgatt acgccggtat
     1861 caataattgc tttcttcatt ttttacgaag tttagttttt attttttcag aaataataga
     1921 cttatattct acagaaccat ttttatttcc agtcttgact ttgccgaagt attgagttcc
     1981 tagctcatag ggagtttttt tattttcttc gtagctgtct aaataattta gaatgagttc
     2041 tttaataatg tcgcttttag tgcgtgactc tatctttgcc accttatgca atttatattc
     2101 tatttccctg ttaagtctta atgttgtcat gtattgcatt ttgtattact aattagattt
     2161 gtcaattacg aaaagtatat gctcagggga cgtgttttat gcaaatcttc gttccgatta
     2221 atccttaata tagctttgtt ctaatttttt taaaactgcg aatgtccaat ctgtaataaa
     2281 ttgcacagat tctcttgagt tcgaactgtc cgaggaattt tctattaaaa ttttctggca
     2341 gtattcctca tcaaatgaaa ttggttctcc aaaaacaatt ttcactttct ttccgactct
     2401 aggtaggatg gcgcctaccg gtagaatttc tttctagcgg ctatcgccta atttgcatcg
     2461 aacttctccc ataattgtct cccatttcta tcttacatgt taaaggctta aagcaaaaaa
     2521 ggattgcgag tctttcataa aaagatgttt tgaactaaac ggggtaaaat ttcatcaaat
     2581 aaaaatattc ttttgtggca attccttttt agccgcttcg aaaagcttgg tatctgaagt
     2641 tatgaactgg tcggatttac ttatttttgc ggcagcaagt tggattccgt ctaacgatct
     2701 tagtccatac ttttttatta cctcgattac caattcttcc aatgtcgaat taaaacttat
     2761 aaattcataa tctacagaat cttcggtaaa ttcagcgagt gcgatttcat aagattcttg
     2821 tgttaaaaca tttgtattaa caagcctttg taaagcagat tgaaattcaa tcctagtgat
     2881 cggggataaa gcaatttcat cagaattttg aaaaagttct gatacagcat cagatccgtc
     2941 ctcttcaata tattttttta caagagcaga tgtatcaaag aaggatttca agtttcattc
     3001 ctcatttgca ggattgtttc ggaggctgag ttcttatttg ataattttac ccttcgcaca
     3061 tttctctgcc aagatttttt gcgaatgata atttcaacat cagctttcat atcgggaata
     3121 tttttatcag taatagtcac ttttccgtta tcgtaaaaac catgtagaat aatcataata
     3181 gacctcattt cataaaataa acatctttag gaaattgtca aagagaaaat ggttcagaaa
     3241 gagttcttct agtctttctt tttatagcgt tgcagctttc tgtctatact tttctttcta
     3301 gcgtctgtcg gctaatttgc atcgaacttc tcccataatt gtctcccatt tctatcttac
     3361 atgttaaagg cttaaagcaa aaaaggattg cgagtctttc ataataagtt gttttgaact
     3421 aaacggggta aaattttatc tcaatataac agaggaatca tacaatcaga ttatccaaat
     3481 ctttcctgcc tatcaaggat tagtcgaaaa atgtgctaac ataattggaa aggatcatgt
     3541 aaatacaaaa gcaaaaattg aggattacaa tgtcccaaaa ggaaattggt gccagcatct
     3601 ggttgtccaa atttctagtg agtatgacta tggtaattta cattggattg caagggatgg
     3661 aagggggttg tattttgatc ctatttgtgg cacaagtgga aatgaatggg gagatttaac
     3721 aaccttcgat ccgattcaaa gtaggttcct aactctaaaa acaagtgctt ccatagttat
     3781 gagcgacgtg gttcattgca aattacctac acaattggtg aatctagaat acaattggat
     3841 tggcttttgg attgattaca aaaagaaaaa tgattgaaat agtgagggag attttatgat
     3901 tacagcaatc aaaaatgctt atagtgaata ccaaaaattt tggataaaac tttttatcca
     3961 aaaatatatc ccagaagaac aaataacaaa aacactagta gaggatttga accaaaaaat
     4021 tatttttcac aatctgtatg cttttcgcat aacttctgtc attgtggttt tgattgagtt
     4081 agttttgata tttactttcg atttgccaat gctcaagtcc gcaagtgcag agaatcagtt
     4141 tttatattta gggtatttat attctcatat atcgattatt gctttatctg ttttttgtaa
     4201 ttttattctc tatctaccga tttctgcata caagaaatta tgctaccatc atggaatcat
     4261 tgtaccttta tattcttttt ttcttttggt tatttgcgga tatatggatg ctttgaatta
     4321 ctactctacc aaaactatga atctatttat cattgtgatg ttaatgcaac tagttgtttt
     4381 tattttcagt atgcatttta tattattcca tctcattata aattttggac ttttgatttt
     4441 tttcattgat tattacaaat ctttcgatgc tggtgccttg aactcgatta tcaatattgg
     4501 gatattgtct attttcgctt ttgtaagtgg atatctaaga ttccgaggat ttgttagaaa
     4561 cgaaattatt gaaaaacaaa gacaagctct cgatgtgcaa atgaaattga ccaattcctt
     4621 tgaacgattt gtccccaaag aatttttatc atttttgaat aggacaagcg ttttggatat
     4681 agaattgggt gatcaaaccc aaagggaaat gactgtccta ttttcggaca ttcgcagttt
     4741 tactacactt tctgaatcta tgagtccaca ggaaaatttt aatttcatca acgcctacct
     4801 ttctagaatg gggcctcacg tgagacaaaa ccaagggttc atagacaaat acatagggga
     4861 tgcagtgatg gcactcttcc caaataaacc ggacgatgcg atcaatgcag gaattgaaat
     4921 gcaaaaagaa attatactct ataacaaaaa tagaacagaa aagggactta cacctattag
     4981 cataggaata gggattcata caggaaattt gatgctcggt attattggag aaacggaacg
     5041 gatggaagga acagttatta gtgatacagt aaatctagcg tcccgtttag aaggactgac
     5101 aaaattgtat ggtatcacta ttatgataag cgaagatact ctattgaaag tagaaaatat
     5161 tgaaatctat aaatacagac ttttagacaa tgtggtagtg aaaggaaagc aaaacagcat
     5221 tcgggtctat caaatctttg aaggaatgtc tgaaagagaa gaagaactat tgcaagcaac
     5281 gaagcttagt tttgaactgg gaattgaagc ctatttggga cgcagattta cagaagcact
     5341 cgaactattt caaaatgtag caaatgttta tccagaagat gtgcccactc aaatctatct
     5401 aaaaagatgt aacaaatcca tcacagaagg tgtgagcgat gactggacgg gaatcgaaag
     5461 aatgacggaa aagtaaatat ttaggaaatg gaatttttct ggaaggtcta ttctaaaaat
     5521 tggggatgaa tgataacaag ctgatagtaa aattccttcc ctacatctga atgtatcttg
     5581 acaggaaaat tcttactgac tattttttaa gtatggttga agttgaagtt cccgtctacc
     5641 gtttctcata cctttttaaa atggttgcaa ttccttgacc gacaccaata cacattgtaa
     5701 ccattccata gttgaggtta cgccgtatta gctcatgcga aagggtaagc gccatacgag
     5761 caccgctggc tccaagtgga tgtccgagag aaatacctcc accgtttaca ttgacttttt
     5821 ctttttctag atttaattct ttgattgtgg ttaaaaccat agaggcaaat gcttctgaga
     5881 tttcccagag atcgattgtt tctcttttta gtcctgctct gtgtaaggct tttagagaag
     5941 ctggaatcgg tccggttcct agtaaagaag gatgcactcc agatacagca gaacttacta
     6001 cttcggctaa tggcgtgtag cccctctcta aagcatagct tttctttcta ggaatttgca
     6061 tcgaacttct cccataatcg tctcccgttt ctatctcaca agttaaaggc ttaaagcaaa
     6121 aaaggattgc gagtctttca taataagttg ttttgaacta aacggggtaa aatttttttc
     6181 cggaaatatc tattaaataa ggatcaccgt ttaagtcatt tttatctatt actaataatt
     6241 tatctggaaa aaagtcacct tctacataat cccccagatc attttgattt aaaaaatctc
     6301 catgaatttc taaaacttca tagttgtttg tttgtctgtc gccttcgagc gttattagtt
     6361 gtgacatata aaatataact gcgcgagctt aacaacttcc gcttttacct ggtaggaata
     6421 agcccacatt tatattttat ttgattttga tttttaatgc aagcagagaa taataagcat
     6481 tatccttaat gatacaagtc tgaagcaggt gtgaaacaat tttttatttt ttttctggaa
     6541 atacttttcg gaatccttcc ttgtataaat attttccaaa aacacttcta tttggataag
     6601 tcgcgtcagc cggtagccat aacccacaaa ataaaatttc aggatagaca tgatgagcat
     6661 aatttccctg tccacaaatt actattctat aaggtatctt gttttgaatt gctttactta
     6721 ctaacaatat ggttttgtcg tcgcaatctc ttgaacctgt ccaatcattc aaaatagtat
     6781 agcaaggtct agacaatgtt tctttaccga ttggatcagc aacataagga agcgccctta
     6841 caagattata aaatttaaaa aaatcccagt ctttgaaatt atctaaatct gatacatatt
     6901 gtttggcgat tctttgaatc tcgcttactg tttgatcttg attaattaat ttttgttcta
     6961 tggctttcat gttacattaa ataatcttga aaatataata tgataaatga tgcaaataaa
     7021 gaagataaaa taaatactaa taaacattgt aaaataaatt tcataattat aacccttttg
     7081 atttttcaaa taatttattc caagtattgt cattaatcga atgtaaataa tttaaaaaag
     7141 tttcactttg ccaaccattc catttagata ttgattcagt aggacaataa actatttcta
     7201 tctcattatt ttctaaaata ttaacaatac aaccatcagg aattttataa actaaaccat
     7261 ttttaagtga aggaaaagta ataccgtcaa cccgttcatt aggggtttgg tttggcatta
     7321 aaagaagtgc ttttccgtta ggatcatttg ttggatcttc ttcaggttta taaaatattg
     7381 tcaatttact aaaattattc tgaattttcc ccccaaaaat ttttttccta aatatataga
     7441 ttgctaaaaa tccaaaaatc gatacaaaca ttaaaatatt ttttttattc atgttttttc
     7501 ctttctccta ttttataaga ctaaactatt tttttcaact tcttttcttg ttctaaaatt
     7561 actggcatca aaggtatttc aggatcacag ataattttat ttactgcatg tatccatctg
     7621 catctttcta taatgtctct atgaattgga tcaaagattt caactataga attttccgtg
     7681 agttcttcct tgtcttgctg tatgagtatt gcaagtaaaa tatctttgtt ttgcgttgtg
     7741 gtatatagag acttattttc ttgcattttg atttgactga ttacaaaaag atttataaat
     7801 tgaaaagatg aatgaaaatg ataaagatat tattcgtgta gtaaaaatat ttgatatgag
     7861 aacaggaaaa ataaaagaaa ttatccattt ccaaattaat aaaaaatatt ttcttaaaag
     7921 aaaaggaaaa ataagagaat gtataatatt agaatttttt tcaaaagaaa aacattctaa
     7981 agccaaaata atttttagcg atacacaaaa attaggagaa gcttttcttg atgaattgca
     8041 cgaatcccca gagaatacat aaataaaatt cattaattaa ttgcctttaa ttttctcggt
     8101 tttttatttt tgttaaaatc aggaaacttt aatgagaatt ttaaaatttc aatacatctt
     8161 aactttccct tttcatctgc atgaaaaatt aaatctttat aaatttgaaa agaaattaat
     8221 ttccctcgat atttacttaa atatatttta taagaatcat tttttgaagt aaaattagaa
     8281 attatcgtat ttcggttatc actctttttt ttaatatttt tcattgttaa ctcaccttct
     8341 taaatgtttt gatttttggg ttataaatag ctgtataatc ttcttgttca aatgaacctg
     8401 aatttttttc tagcaaagcg gaatatttcg gcgtttcatt tgttttgaat tttcgatttt
     8461 ctaaaacttc taaatatttc tttaaaccaa aaattctaat atatgtatcc gaagcgccgc
     8521 ttgtagtttt ttcaacatag ttttctaaat tctgtaaatc atatactaac tgaggaactt
     8581 ttaagggagt ggcttttttt gtattatgca aaaatttact atagagtcta tcgacttcaa
     8641 ataaatgttt atttttttgt tttttgctta acggtttctg agttagtcga aacattccaa
     8701 gaatggcttc ataaggatta aaattttcat tgtttagata attgtatgta aaccaattcg
     8761 ctcgacctag tttcatttct atttccattc tattccaaac atcgatagaa ggatctattt
     8821 ctattccatc gtttttacat ttagctttct tatcgtaata tctaaaaaaa gtttccgcgc
     8881 ctctatcccc taaataaatt gtttttccag attctactct atcgcgtaaa ttaaaaccgg
     8941 ttgtattcat agagcttact agccgcgtaa cgtaacaacc ttttttaatt ttgtattcta
     9001 ataaatctaa atcaatacaa ccttcgaagt catctaaagc aaagtctatc cttgtaattc
     9061 ccgccggtcg tgatccttct cttttagttc gattccattt acaatttttg aacatccata
     9121 ttacaaaaaa aattataact tcaatcgaaa gagttttcca aatgcatgta gaatgtacga
     9181 taaagctttg tctctgcctt tattcgcggg tatgactgat gaagatgtag aagacgtgat
     9241 ttttgccgtg caagatattt tacgctagaa cgttatattt tttttcgata tattgtcaac
     9301 tttggacttc ttaatttata tctattttct ttgacagttt atatattttt gcctgatatc
     9361 tgtatggaat taaaggaaaa tatgctgaaa tttggtattt ttggaagaga ttatattcag
     9421 ttatttctat aacgtgaaaa aaatacttgt attctagtat aaatggaatt aaatcataac
     9481 taacaatttt ctatttttat aaaacagtat agttttaaaa caacaatagc aattagcggt
     9541 tacacaaaga gatttttcta tttttgtaat tctgtagaat agatttgaat ttaaaaaaca
     9601 ttgtgtttag ccaagataaa tgaataaaac tgataaatca acaaagccca ttctctatgt
     9661 aggaattggt gcatctgcag gtggactaga agccctagaa gatttaatag ctaatatgcc
     9721 tatcgatacc ggaatggcat tcatcattgt acaacatctt tcaccagatt ataaaagtat
     9781 gatggtggaa ttactttctc aaaaaacaaa aatgaaagta atgaaaattg aaaatggaat
     9841 ggatgcgtta cctgacactg tatatttaat tccacctaaa aaaaacctga ctatttttca
     9901 ctctaaatta ttacttacag accatgttcg agattctcgt ttgaatctac cgattgatat
     9961 tttttttcaa tctctcgccc tagatcaaac tgacaaagct gtaggaatta tactttctgg
    10021 gacaggaagc gatgggacgt taggtataaa agcaattaaa gaacaaggtg gactggttat
    10081 tgttcaagag ttagcttcca gtaaatttga tggaatgcca aaaaacgcga tcgcaagtgg
    10141 atttgtggat tatattttac cttgcgataa aatgggatct atactttccg attatgtaag
    10201 gaattccgct agcctaaaaa atttaactat ggatggagaa agtccgagta gtgatttagc
    10261 aaaagtgtta agcattattc ggtcaaagga aaatctagat ttttcttact acaaacccaa
    10321 aaccataaaa cgtagaattg aaaaaagaat aaaatacaat cgattcattt catacgaaga
    10381 atacatacaa ttattaatta agtcaaaaga agaatcagaa attttagcaa atgaattttt
    10441 aattggggta actcattttt ttagagataa agaagcattt gaagtattgg gagaaaaagt
    10501 tatccctaaa atatttaaat atctgaaaca aaatgatact gtacgaatat ggagcgcagg
    10561 atgctctacg ggggaagagc cttatacact tgctatttta ttctctgaat acatggagat
    10621 gaattcccta aagtttgatt taaaaatttt tgcttctgac ctagataaag aagcaatttg
    10681 aaaagcaagt tcaggtattt atcccgaaag tattgcggtt gacgttcctg aatttttgtt
    10741 agagaaatat tttgttttta aaaatggata ttttcaagta attgataaga ttcgacaaat
    10801 ggtagtattt gctaggcata atataatatc cgatcctcca ttttctaaaa tcaatttagt
    10861 tgtatgtaga aatctactta tttattttaa cagtgaaata cagggtaaaa tattaaattt
    10921 gtttcaatac tccctttaca atggagggtt tttgttttta ggaagtagtg aatcaattac
    10981 gaatgccaaa gacttagagg ttgtagacgc taaaaataaa atatataaat acattgggaa
    11041 ctcaatcagt caaatcccaa gtcaaatttt actgccaaga tatgataggg aaaaaaaaca
    11101 agctcttacg gaaaaactcc agagtatttc taaagtttct tcgcaaaaga tattcgaaag
    11161 tgtatatgaa actgcgatta atctatttgc acctcctgga tttataatta attctgagga
    11221 tacggttatc cattttttta gtggaatcca acaattttta tccttgccaa gtggaaaacc
    11281 aacttataaa atatttgaaa tattagattc acagctttca actatcgtct ctaatttaat
    11341 atataaaatt agaaaagaaa aaaaagatgc ttctctcaaa aaaatccatt ttaaacttcc
    11401 agataaaaaa atcataattg atatagtagg tagatattta ccaaatacca aaaatacgac
    11461 cgattatatt ataatcacaa ttgaagattt taaatcagtt gcaaatgata gtattgagca
    11521 aatttctgaa tcgtttgatt acaaccaaca actcgaagaa agagttaaaa tattagaaaa
    11581 ggaacttcaa tacaaagatg aaagtttaca gactactata gaagaacttg aaacatccaa
    11641 tgaggaatta caatctacaa atgaagaatt agtttcctct aatgaagaat tacaatctac
    11701 aaacgaagaa ttgcaatctg tcaatgaaga gttatatact gttaattccc aataccaaca
    11761 aaagatagat gagttaacag aagtatcaaa tgatattaat aatttactaa agagcacaaa
    11821 atttgggtat ttatttttag ataataaatt acagattcga aagtttaccc ctgaaataac
    11881 caaaattttc aatattatgg aaatggatat aggcagacct ttcgctcata tttctaatca
    11941 aattaactac caaagtctac tcgcagatat ggaggaagtt ttagattcaa taatagagaa
    12001 agaaattgaa gtgaaggaca aaattggaaa ttcttattta ctaaaaatgc atccctatag
    12061 attccaagat aattctattc atggagtagt attgactcaa atagatatta caaaccaaaa
    12121 aaatactcaa ctgcaaaatc taaaactagt cgaagcagtg gaaaatagtg taaatggaat
    12181 actcattact aatctagatg gaaggattga atacgttaat tcaaggttta ctgaaatgac
    12241 aggatattcc gaagaagaag tagtcggaaa aaatcctaaa attctaaaaa gcggatatca
    12301 cagtaaagat ttttatacaa acctttggaa cactatactt tctggtaagg tttggacttc
    12361 ggaaatgaaa aatagaaaga agaatggaga aatttattgg gaggaaaata cgattagtcc
    12421 tatcaaagac aaaaatggta aaataataaa ctttctctcc ataaaacatg acatttctga
    12481 atctaaaaga atagacactg aactaaaagt aagttcaaat actctgaatc gaatacaaag
    12541 attgagtatg actggaaact ggacttttca ttttgtagat aattcactca attggtcaga
    12601 agaattatat agaatttttg ggtttgaacc atttagcttt actccaacct ttgaaaattt
    12661 tatttccatt gtagacttgt cggatagaga gagagtaatc agggaatttg aaaatgcaaa
    12721 agaacataaa acaaattatc aactatcgta taaaattata cagccagctg gagaagctaa
    12781 gtatatagaa gagatagtgg aattttattc tgatggaagt ggatatttat tagggtgtat
    12841 aaaggatata acgcaacctc attctctctt acaaaatgaa aaaaaaataa atgaagattt
    12901 gcaaaactta tcgagcgaag ttaaattaga aaatataaat ttaaattata caaattcatt
    12961 tttgataaaa tcaatgcctt ttccttttgt ttatggtaaa attattttag atgaaaaggg
    13021 aaattctgta gactatgaat ttatagaaat caatgaagcc tttgaaaaaa ttacaggttt
    13081 agcaagggaa aatgtattaa ataaaaatgt aaaaagtatt tacccagata tagaattaaa
    13141 ttggatagag aaattaggaa aagttgcatt atcaggagat tccctaacat ttgaagaata
    13201 cagtaaaatt actaattcta cttttatagc gaaagccttt agccctaaaa tagggtattt
    13261 ttgttcaatt tttacagaaa aggaaaagag ctctaatgtc tgactttcta aaagaactac
    13321 gtgaaagagc tgagaaagtt ttaaaaaaat cagatataga tcctgaaagc ttaacaaaag
    13381 atgaaattct agcaattata cataatttag aagttcatca aattgagttg gaattacaaa
    13441 atgaagaatt attaaatgct caaagcgcat taaaggtagt caaccggcag tatatagatt
    13501 tgtttgagtt agcgcctgtc ggttatataa agttaagtca cgaatttgga atcttagaag
    13561 taaatcattt attttgttca tggttcaaag atacaattga ttttcataaa acttcctttg
    13621 aaagttttat ttcccatact gatcaagata ttttttttcg cgctaaaaga aaaattcaaa
    13681 aaacaaaaga aaatcagtcc atcgagatta tcttaagaaa taatctattc gttaaattgg
    13741 atatcaaatg ttattttgat gattcttatt ttttaacgtt aacagatatt tcagaattaa
    13801 aagaaaaaga aaaaaaagtt ttagaattaa acaatcgatc aatggctctt ttagaaggat
    13861 ttccggattt agttttattt ataaatagcc gaggtgagtt aatcgaatca gctgcaaatc
    13921 aaaggaataa tgctatccgg tcactcaatg atatcttccc caaagattct atttctatca
    13981 taaaagaaaa aatacagcga gcggtgtttg atggattttg tatttttgaa tttcaaaaaa
    14041 ccaataaagt tgggaaaatt gaatatcttg aaactagact tgttaaaagt aatcaagatg
    14101 agataattat cattatccgt gatttaacag ataaaataca agccaactta gaattagaaa
    14161 aaagcaaaaa tgaattggaa ctcatttata ataatattcc aatcctcgtg agtcatgtgg
    14221 ataatgattt taattatatt tatgtaaata aagcatacaa tgatttcttt ggcattgaaa
    14281 aaatcattgg aaaaaatata ctggatgtga ttggtcctga aagttttgag aaatctctac
    14341 caaatttaaa taaagcgtta aacggtattt ctgtacaata tgaaaatcga atttataatt
    14401 cacaaaatat tccttatgat gtaaatataa attacattcc taacgtttta aaaaataagg
    14461 tcgatggaat ttttattatc gtagctgaca taacggatag aaaagtctat gaaagaaaat
    14521 tattggaagc gaaagaattg gcagaacaag caaacaaagc taaaagtatt tttttagcaa
    14581 ctatgagcca tgaaattaga actccaatga atgcgatcat tggatttact gatttattaa
    14641 atcaaaccgt acaggatgag acttctagaa attatgtaaa ctatatttct tctagtggga
    14701 atctattatt gaaaatcata aatgatatat tagatttatc taaaattgaa tccggtaaaa
    14761 tgattttaga gcctatttca tcggatttaa tagaggtttg caaagaaatt atagatatat
    14821 tttctcgaaa ttttaagaat aaaaatctta aatttatttt tgaaaaatcc aaagattttc
    14881 ctaaaattat ctatctagat gaattaagat taagacagat tctatttaat ttaattgcga
    14941 atgctgaaaa atttacgcaa gaaggatttg ttgccgttta tttacatttt aattatcatc
    15001 aaaatccaaa tatgaaatta ggagatttgg aaattagaat tgaagattct ggaattggga
    15061 tttcgagtga ggacttagct aatatttttt cagcctttac acaagcaaag gggcaagaca
    15121 gaggacgtta tggtggaaca ggacttggat tgactatttc taaaaaatta attcagctga
    15181 tgggaggcga aattgaagta acctctgaat tagggaaagg aagtcaattt caaattttac
    15241 taaaaaattt aacgtatgaa gatatttcgc acagaacaga aaataaagat tctacatttg
    15301 atctaagtcc attcaaaaat aagaagtttc taatcgctga tgatgtagaa ttaaatctaa
    15361 tattgttaaa gcaaaccttg accaaattta attttcaaaa tgtttaccaa gctgaaaatg
    15421 gactagaagc atttgagata acgaaaaaag aaaaaccaga tttaatcttt atggatatca
    15481 tcatgcctgt tatggatggg atacaagcta cccaaaatat tagaaacttc aaagaactgg
    15541 aaaatacaaa aatcgtagca tttacagctt ctgtagattc tatctctaaa ctagaatcaa
    15601 tgggattgtt tgatttagtt ttattaaaac cgatcaaacc aaaggaaatt ttggaagcca
    15661 tttataaatg ttttaacgca gaaaaattat cttaattatg aataatgcaa ttgaatgagg
    15721 atgtatgaat tattttacca atcaactttt aaaaagtcca acaggaattg aaggattaga
    15781 tgaaatctta gaaggtggaa ttcccaaagg aagaattact ttaataacag gagatactgg
    15841 aacaggaaaa agtttaatgg ctcttgagat gattgtaaat ggagctacaa aatttgatga
    15901 acctggacta tatatttctt ttgaaaatga attaaaagaa atatttcaag acttacatag
    15961 cattgaatgg aatttagaaa atttatcgaa agagaacaaa atttctttaa aacatattga
    16021 tgttcaagtg gatgaaacag aaagtatagg ctcttttagt tttgatattt tacttaagag
    16081 aattgaaaaa caaatcaatg aaattggtgc caagagagtt gtattggata cactagaatc
    16141 aatctggagt gttgtagggg tagaaaatat ttcaagatgg gagttgcgaa gactttttaa
    16201 atggttcatt gataaaggaa ttacgctcat tgtaacggga gaaaaaagag atggaacttc
    16261 tcattttttc ttcaatgaat ttatttctga ttgtatcatt caattagaac agaaaatatt
    16321 cagtgaaatt gtaaccagac gtttgcgtat tgttaaatac cgtggcactc ctcatggtat
    16381 gaatacatat ccttatgtaa ttacttctaa aggaaataag gtatttccaa taacgtcaat
    16441 gaatcttgga catcatgtct caactgagaa aattagtttt gggatggaag aaatggataa
    16501 aatgttggga gggggtatat ttaaaggaag cagtgtattg ctgagtggta cctctggaac
    16561 tgggaagtct acttttgcaa atttttgtgc taaccatatt tgtagaaatg gtggcaaagt
    16621 actgtacttt gcatttgaag aatctgcaga acaaatttta cgaaatatgg tttcactcag
    16681 tttagacaat agaaaatttt atgacgaaaa acaatttatt atttttgcaa gacgacccaa
    16741 ttctatggga ttagagcaac atttactttc gatattttca cttgtagaag agcataaacc
    16801 tgaattggta attttggatc ctatttccaa tttttcttta aacattcatg atgataatat
    16861 taaatctttt ttgatgagac ttgtagattt ctttaaacaa agaattataa ctacaatatt
    16921 aacttcttta attcgaggtt caggagtttt ggaagaaacg gatgaaggag tttcttctat
    16981 tatggataca tggatttccc tccaaatggt tgaacaaaat aatgaaagaa acagaataat
    17041 taaaatccta aaatctagag gaggagctca ttctaatcaa ttgagagagt atatactaac
    17101 ctcagacgga gttcattttg aagatgtata tttaggagcc aaagggatgt taacaggttc
    17161 tcaaagactc attgaaatag ctaaaaatga agcagatgaa aaattgagac ttgataagtt
    17221 acagagaatt agagatgaga ttttgatttt agagaataat ttcgaagctg aaaaatctat
    17281 actctattca aaatttgaaa aagaaaaaaa agagttagag gaggaattac tcaaaggaga
    17341 gttaattaca aaagtaaaca aatcagacaa agagcagtta atcaataaac gaaaccaagg
    17401 attgtaaata gaatggctga aatttctgta aaaatattcg ttactactaa agacttagca
    17461 tcagatataa tccaagcttg gaaagaagaa ttaaatctat taaagaatct tagttttact
    17521 ttcgaaataa tagattttga ttccaatccc caacaaattg aaaagtatga tattctattt
    17581 tcccccacaa ctctaattac ccttccagaa ggaaagaatg tccgattcat cggatataat
    17641 aagaatatcc tagtttattt gagagcttat agtttaaaaa ttcaatccga aatagataaa
    17701 accatggcgt cgagtcatct agatcaatcc ttggaaatga aaataggcat agctaaaatg
    17761 agaaaattta tgaatcctaa agtttgaaaa tcctactaat taaattgtat tcaatttcat
    17821 tttcctctga atgaaaagta gaaacttcta actactgtac taagtttaga atgacatcta
    17881 gcctaaccgt ttttttaaat cagggaaaat ttttctagaa ataggaattt ctttctcatt
    17941 ttttaagact aacgaatatt taccgttccc attctttttt ataaatttag attctcgcaa
    18001 atttactaga taggatttat gaactctata aaaatgagaa gggagaattt tagaaaggga
    18061 ttccagagtt ttacgatgag tatccgttga taaatttgct ttctaggaat ttgcatcgaa
    18121 cttctcccat aattgtctcc catttctatc ttacatgtta aaggcttaaa gtaaaaaagg
    18181 attgcgagtc tttcctaaag agatgttttg aactaaacgg ggtaaaattt gataattgga
    18241 taaatatgaa gcactatctt gtgatggtaa tctcaagcat tttgatgttt ataattggac
    18301 tatatactat taataaactt gaaagtgata taagggatag tatatgatga ataaagttat
    18361 agaagtaaaa aacctgacaa agtcatttaa gctttttcat agtaacaagg atagattaat
    18421 taatattatc ccttttataa ataaaaagtt tgatgtgcat acttctttga ataatatctc
    18481 ttttgatgta aaaaaaggtg aatttgtaag catcatagga aaaaatggta gtgggaaaag
    18541 cacacttctt aaaattcttt gtggagtttt gcaaaagaca agtggagagt atatagtaaa
    18601 tggttctata gtatctttac ttgagcttgg tactgggttt aatcctgagt taagtggtag
    18661 agataatatc atagtatctt ctaagacaat gaattttgat ataaactata tttattcaaa
    18721 gatgagtgaa attgaagctt ttgcagattt ggaagagtat tttgatatgc ctatcaaaac
    18781 ttattcttct ggcatgtatg tacgacttgc aatgagtctt tttttacact taaatccaga
    18841 tatatttatc atagatgagg ctttaagtgt cggagatata ttttttcagc aaaaatgttt
    18901 ttctaaaata gagcagatga gaaaaaatgg tgtggcattt ttatttgtaa gtcatgatat
    18961 aaataccgtg cttaatatgt ctgatcgtgt attacttctt gaccatagta agcagatata
    19021 ttttggtgat aagcatgaag cctgtaagct ttactatgat agtgataaaa aagtactaat
    19081 tccagaaaat gaatcagatg aaataaaaga aaaaatagaa gtatccatag aaaacaatat
    19141 tttaaatggt aaaaaacaaa tcattcatag aaagggagca actcttgact cacttcaaat
    19201 tgtagatgaa tttggaaacc ataccatgca agtggagatg acaaaatctt tgaaatttat
    19261 tataggatat aaagttgatg agattttgga caatcctgca attgctatta gtttttataa
    19321 caataaaaat atgcttataa caactattgt atatgattta gtcgttgata aaagatttat
    19381 agaagttgca atcccattta tgttggaagc aggagaatat acttttaatc taacaatagc
    19441 tcaaaaagta aagaacagag gagtgtctca tcaagagtat acaaatgttg gaccaatatc
    19501 tgtaacattt gattttaaca gcaatgagcc ttccttctat ggcttggttg gattagatta
    19561 caaaataaac tttaaggatt aaaataaaag atgagacaat atgaaaaaaa tataacatca
    19621 caatttggag aagatggaat aatagaatac ttgttttcaa aaataggtgt ttccaatgaa
    19681 ccttggtgtg tagaatttgg agcgtgggac ggtgagtact taagtaatac atataatcta
    19741 gtattcaatc agggttttaa ttgtgtctat atagaaggcg attccaataa agccaaagaa
    19801 ttaagaaata aagtcagtaa gcttagtaat caaaaaatat ttgtgttaaa taactttgtt
    19861 gaaagtcaag gagtgaatag tttagataac ttattgggac agactgatat acctaaagag
    19921 tttgatttac tatcaattga tattgatggc atagattatc atgtgtggaa aaattttaaa
    19981 gattacaacc caaaaatagt tttaatagag cataatccta caattcctcc gcatttagag
    20041 tatataccag atgaaaaaag ttctcttgta ggagcatcta cattggcact ttatggatta
    20101 ggtcaattaa aaggctatga attggtctat tgtacagata ctaattctat ttttgtaaga
    20161 aaagattttt ttcaactttt caatataaat gataatacac cagagaagct tatgaaaaaa
    20221 gatcatataa cttatttgat ttctaaatat gatggctctt atttggtaaa taaagaaccg
    20281 gctttcataa atatttttga taacaatatt tttaataacg ctaaaaaaga tattccaata
    20341 tggcaaaaac tttttctata ttataagtta attaagaact ttagaataaa aggtatctat
    20401 ataagagaag aaaagttttt atgataaaaa atttatacat aatatttata aagactattg
    20461 cagagttgtt atcacctcta cctattgtta tagaacaaaa aaagtgggaa aaaagaaata
    20521 ttgctttaag actgttgggg ttaaatattg gtaaaaatgt cagaatagga ccagacttcc
    20581 taacaataaa tggattagaa aataatatat caataggaga ttatactgat ataggatatg
    20641 ccaataagtt ttattcattc aataaaatta ttatagtaaa gtttaatatg ttcgcagcaa
    20701 atattgaaat tgttaatggt ggacataatg taaataattt agaaccattt tctaaaccta
    20761 ttattatcgg aaatggttgc tggtttgggc atggagtaaa aataataaag ggtgtaacaa
    20821 taggtaataa tgtgattata ggtggtggta gtgttgtgat agatgatatt cctgataatg
    20881 ctattgctgt tggggttcct gcaaaagtta taaaatatag agaattacct gataaagttt
    20941 ggcatttggg aaatgaatac ttttgtccaa aaacatttga attaattaag gattaaaatg
    21001 aatttatatg aaacaataaa taatttattg aagaaaccag tttatctgat agacgtaggg
    21061 gcttacaaag gtgagtttac agataatttt ttaagtaata taaataaaat agaatttata
    21121 gatatgtatg aaccacagga tagtagtttt gtagatttaa aaaataaatt ttcaaataat
    21181 aaaaatatta atatttataa ttttgctatt ggaaatgaaa atatccaaaa agaattcttt
    21241 tattttgaga atgaaggata taaaagttct ttactcaaac ctattgaggg gggggtaaaa
    21301 tgtctatagt agaaataaaa actttggatg aaatttacaa aaaagtagaa aacgcttatc
    21361 taaaatatga ttattttcta aaattagata ctcagggtaa tgatttatct gttttaaaag
    21421 gtgcaaaaaa ttttattaaa aattctcagc caattatttt ttgtgaatta atttatgtcc
    21481 ctttgtatga gaatcaatct tatgcacatg aaattataaa ttatatgatg gaaaatgatt
    21541 atgtcttgct ttctaggaat ttgcatcgaa cttctcccat aattgtctcc catttctatc
    21601 ttacatgtta aaggcttaaa gtaaaaaagg attgcgagtc tttcctaaag agatgttttg
    21661 aactaaacgg ggtaaaattt tacagcattg cttgaggcgc tagtagtatc agatcgacgc
    21721 gtatttattt aaatatttct ttgagatgaa tttcttttcc aaaaatggaa atgatgtctt
    21781 gagcgctgta agtaatcttg ttagaataat catctccatt cggattttgg taggtttcaa
    21841 tttgttgttt gcttagattg acaatccaat agttttcaat ttttgcttct gcataaatag
    21901 gaattttttc tctatcgtaa ttcaaagatg tctttgcgat ttcaactact agcaaagcag
    21961 tcgtaggatt tgcgtttcgg aaatcttcta aactcccact gatgattgat atatcgggct
    22021 ccgggactgt gcgtaaaagt gtgagtggtt tctctgagcg gataatcgta tgcataggtt
    22081 tgattttaga tagtgcatca aatagaatat ctgaaaaata actgtggtcc ggtcctttcg
    22141 tcattttatg aacgattact ccttctatca attccgtatt ctcaggaatg actccaagtc
    22201 caatcatttg catatatttt tcaaccgata cagttgaaaa caatttacga acttctggat
    22261 tttctagaac aggaaaagac atgattagat agtaggtaga attaagtttt tgtcaagaaa
    22321 ctaattgact tagattagta ttatccagat ggcttggaat ggtctatttt atccattggg
    22381 tttactcctt ttgaatgctt actaattcag gtgcgataaa aactaattat ttctatctag
    22441 taaaccgtct ttgatctaga tggacttctt cgacctgagc tacaaggtag actcgggatg
    22501 aagtggtatt taccagaata cattcagcct taattgaatt cctctcgtct cacgcaaagc
    22561 cgctaagccg caaagagttt tctaaaagaa aggaagagaa tgtttgttgt tttgtaaaat
    22621 gggctcatgg gttactcctt agttgcctct gcatctttgc tttctaggaa tttgcatcga
    22681 acttcctcca taatcgtctc ccatttctat
//
LOCUS       DAOQOS010000090        21425 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_1184_length_21425_cov_5.437061, whole genome shotgun sequence.
ACCESSION   DAOQOS010000090 DAOQOS010000000
VERSION     DAOQOS010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 21425)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21425)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21425
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1184_length_21425_cov_5.437061"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(62..466)
                     /locus_tag="PLF66_17760"
     CDS             complement(62..466)
                     /locus_tag="PLF66_17760"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002973585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HMZ65941.1"
                     /translation="MKLVSKKNLSFGSSPDNNDGLQLRITFDEDTKTAYGDYTCPAKF
                     QGAPDSIHPGIIATILDEIMIKINEAMNFETMTGELTVRYLQPAFINEPLHLRGWFVK
                     KNKKVIENRAEIENEIGKIVARGKGKYMEAGD"
     gene            657..3248
                     /locus_tag="PLF66_17765"
     CDS             657..3248
                     /locus_tag="PLF66_17765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000209452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65942.1"
                     /translation="MAPLNTPSSLTLSYDATEILGEQEKSLFLHKRPLLNADTMVKKG
                     HFKSALEIYYRTASKITNQNIVAKIQKNISDIHNYLKSSDSESEKAISEKYSDNRGKE
                     FTESLKELTENLSDSLTEKLNILKQIETLPRKKSENPLFADRAIEEPPIEEDENGIKT
                     LDLGSLEAKPKPKEQKPKDEVEEIQATVEYTNDADNEGLPQDKKPKQAKIKIPKDDLI
                     GSSDKTEVSSQTTEIKNEKAEIKNETNIQNANLQAESLQIDSTKQLQSEVTENTSSKI
                     EPDSYENKPPSKDVIPSSTKKNPDIVEEVKIIKEYIQKIESTVARSDSDEIDQTRIET
                     GWSELEKEPPKNKSDKDYAPQMKKYEVGSIEIPEATEFFTGDFISREASEIQRKEKTD
                     KDSTKEEPFFSPDIANDTNKQARISDSGQTAFNIPAMPPPLPPNINDWLSNLYFSKEW
                     EMFKPLPLKERRSGKERRQNKSNQLPEGIKKDRRSGTDRRKQNENLLHARDAFLKDWH
                     QSQYPPSFPNPYSAGSFPANLGPESSAFVGEASSEKDAHFDPNLRTLEFLPRDPGSHE
                     KLQTPEDLVKVDLPDPEEIILKPPIDQDEYPREEVKSDIADTLKKYIPANEELGLPES
                     DKGLVKIDLPNPVSLKADTNDLPQREVSGEIEVSSKDGTGIVSEAVGKDRSGMGPVPY
                     TPEAYEIPEELKIDLPDPDDFIRTKTGHLIDPFAPEETPDINIIDGDGPKETDESSPE
                     ISEIPELPPPPPEPERTIHGILELKPPEIDDAPFLTLTYDFSKIPHPFKLSKNYSIME
                     YSYYKYKPMLMKAQEFARRKMLKNALNYYRVIKSQNIPPELKNMINRNITDITEFLEK
                     FIMSKGG"
     gene            complement(3268..5082)
                     /gene="pepF"
                     /locus_tag="PLF66_17770"
     CDS             complement(3268..5082)
                     /gene="pepF"
                     /locus_tag="PLF66_17770"
                     /inference="COORDINATES: protein motif:HMM:TIGR00181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HMZ65943.1"
                     /translation="MSVNLLPKRKDVSKEETWLLDDLFLDFKDWEKTFQTLPDTESLK
                     KELEDNFKNKLHQSPEKLLECFQYRDELSRKIENLYVYANLRASEDIADTEANEYCGR
                     IDVRSSELNSQFAFVEPEILKIPDIEKWLESDILKDYKFRLSEIIRFKEHILSEKEEA
                     ILSQLNVVFHVFDDIHSKWNNADLKFETAKDSENKDHLVANSRLLLNLQSTDRVLREN
                     TFNSYYKEISKWRNTITANYYGNMVSGSTLAKINKYKSFIESKLFQDDIPISLYDNLI
                     TSVRNNLDSLHQSMHLRKKILGIEKVAPYDRYVSLFKATNPLKFTWEEGRDLVLEAIR
                     PLGDEYVNIARKGLTTDRWIDRAENEGKRSGAFSWGTYDSRPYMLQTWNGTISDVYTL
                     AHELGHSMHSYYSHKYQPYHYGSYTIFVAEVASTLNEALLTNYILEKHSNSDLAKSVL
                     SESVANFEGTVLRQVLFATFEREAAKIADSGEVFTPDRLDELYYNLVQEWYGEHSLAS
                     DYIKHEWMRIPHFYSPFYVYKYATSYCASLSLSESLKENKDKTKDKIFQFLKSGGSKP
                     SLEILNLAGVDLLKPDAINSAFENYKSNIKKAEEAFLK"
     gene            complement(5091..5477)
                     /locus_tag="PLF66_17775"
     CDS             complement(5091..5477)
                     /locus_tag="PLF66_17775"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HMZ65944.1"
                     /translation="MKKVLSVGQCVPDHSTLCNYFENSFDCQVIKIDSTDEALKELRK
                     NSYDLVLVNRKLDIDYTDGTILISEMQKENELRQIPVMLISNYEEYQREAIELGAVRG
                     FGKLEYGKDSAYEKVAKFLPPKKIHV"
     gene            complement(5478..5873)
                     /gene="msrB"
                     /locus_tag="PLF66_17780"
     CDS             complement(5478..5873)
                     /gene="msrB"
                     /locus_tag="PLF66_17780"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010575949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HMZ65945.1"
                     /translation="MNKIIKSEEEWKAQLSPQEFKVLRKHGTEPAFSGEYNHNYDTGK
                     YYCNGCGNLLFDSKAKFDSGTGWPSFYEPISSEAVETTVDKSFFMTRTEVHCARCDGH
                     LGHVFSDGPKPTGLRYCMNSVSLKFKKEE"
     gene            6234..7574
                     /locus_tag="PLF66_17785"
     CDS             6234..7574
                     /locus_tag="PLF66_17785"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HMZ65946.1"
                     /translation="MMHTKVMIVEDETIVGLDMKNTLVQLGYEVTGISHSGEKAINDA
                     IKNKPDIILMDIQLDGQMTGIDTAKRIKQRFDVPIIYLTSYSDDTTLKEALSTTPSGY
                     LLKPFIPRELHTSIQAALHLKSKDNDTKLARLKYIQSNILKNKDLQNQIKDIRKDPNL
                     THENKLERLLFIKELIRKKRKFEKKFEDLSYIHSSIENLIEDNLFTLEVNIDYPLIFP
                     IKELAYQLNYTEDEIIRMGSLSIPLLIHPVDIESMEKFQDRIIANPSIPVEVEIRIKN
                     KSDHYRCFYVRGIFEKKSNTFGKILYLTRDITWMKSLQSDSHTAREKLVAISNNPYFG
                     VAVLDKNAKFLNVNSTIENLTGYTKEDLNWLHFSDFIGAKHLVSICENFERLKNGKLD
                     IYQAEVLIHTAHQGVVWVYIVLNAIRDPEKNVEKILCTLVDISESKLFSKPKYI"
     gene            7590..10904
                     /locus_tag="PLF66_17790"
     CDS             7590..10904
                     /locus_tag="PLF66_17790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65947.1"
                     /translation="MNLSFLLRIIFFTVLLFTFPIFSDDWSDLDEGEKKEVYGSEKSK
                     KYKMSNIFGEVESWDKHYAVRIIYLYDYTDYPKYNSTQFIPFYYRLNSKIDNREKLRF
                     INYTTEIQKSKVDKSILPLFYWGHNNDLKLNYQLFLPVYYNSSTNNSNLTENKFIFIP
                     IPFYYSFEDKNKKNSTSEKHYSILHGRVLQKKKNSNDTLESSFWFPGFPLMRYTKGQD
                     FYYHYIIPFYGYSRTENLQREETTFLFSLFPFYYSASSYPIDGNNKHSELTHWTLFYY
                     RSLEEDKGGFREYTHKTVWGFPVLPVLYYSSFRQGEGYYKRILTFFHWEKDEKEELNS
                     LSFLPFFFYKKNDYFRIPILFINKDLSSPSASYGKTFVPLLLYYNRWDLSSQTTVLGP
                     WIYTENKLKNESFKTLFPIYWKSESEKKDITLIFPIYANYNDKENDYHFNPIWFSRAN
                     LGTLNPSLSLGKKEGIWYLDTDFTFFYYLASLSFRQTIEKPKFLRDVLNRNKSYEELE
                     KENKEEKVRIKKESKDSSDPKITKRKTITREDSLSFSGYSLLFGLLSYEAADSKRHFR
                     LLPLSWFSWDKESDDKVYVAPLFLWYQSDLLEYLVLFPFYGKQKDESSEKKAFLLNVY
                     ISEFYKDKNWVEKSILWPLANQYTSEDKSGHRILPFYIHKNSKFGESSITNNYTLASY
                     YESRTSKDSEQTTLFIWPTLFYYKSYSYKENPEKPYSKSRFWFTPFFFRSKDNDSSHT
                     NIFWILDFSFYQKKLERGILFPFYYFADGNFGIFPISLNKTGSYFWTFTFFNYYRSTE
                     DGFYYNFLFLLESEKSKRNFETNLLFYSIQYKKSETEFSLNGLWGYGWNFNKSNDTWT
                     DLNIFSYLGGGYRFIGDESIYNISLFYYRATNGSDYQNWLLGNYTRKSKSGFRNNFLY
                     LIDYENFSSDGSYTWDILFTGIRYHNLKNENYFQGAYGYLWNFRKENEIWRDASFLWL
                     GYSNKNSETIYNFLPIFRSYDLNEEHSRIYGPMLLYTSEETGKNFHLGLLGLGYWYNK
                     DERNKEEDTYILLGSLYREYTQKERGFRARGSLWGWLWDYQKEEETSFEKFSILKLFS
                     YTKEADGTKKILGISF"
     gene            10912..12324
                     /locus_tag="PLF66_17795"
     CDS             10912..12324
                     /locus_tag="PLF66_17795"
                     /inference="COORDINATES: protein motif:HMM:NF023459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="von Willebrand factor type A domain-containing
                     protein"
                     /protein_id="HMZ65948.1"
                     /translation="MKYKKYIIPILFILIEVFCSGGNSKSIIIQQSSKPSYFFGNQKE
                     FTSPLKINTIQLPLNVSSESYLNFKNTVTNNDYPSFLYIEQFLNAFQYNLPINSDKNK
                     LSLYTETANSPWSKNRKLLQVSIMGNSKIEKETNSLPKNLIFAIDVSGSMAEPNRMNL
                     AKLALKRFVNNLNPKDFVSIITYSTEAETLIEPTEAQQKSILLNKIAGIRDTGGTNAW
                     PGIQLAYRVAREMQFNKRQTKIVILSDGDFGGVNDEEFFSLVRKERFNNTTISILGFS
                     TERKINTMDKLNDLKLGDSIYIDSIEDARLKLNDNWLQFSSIATDLKLEVKFDSKNVE
                     SFKLIGFDRYNPSKNKEIVDADSSFFYTALYEIIPSKESTPTGELAIFRLSYNTFNKT
                     EELKRTAINSNHSIWDSSDSLRLAAGISALGMVVNDSVTSENIDFYTAIHLIQSTLIY
                     DPDKSRQELLNLVIKLKNMK"
     gene            complement(12340..13821)
                     /locus_tag="PLF66_17800"
     CDS             complement(12340..13821)
                     /locus_tag="PLF66_17800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65949.1"
                     /translation="MLSYFKKEFLYIFIILVFFINIFCEFKKKPSLQYSIPVSNVNVS
                     ATITESDGSTDLFEGTTTDTYTIVLNSNPNMPISIDIGFDSTQIKVNDNTTGLTNINF
                     TPENWNQPQTVTVYALYDNIEEGSQKVNIIHAIKTSNPFQQRVVIGIVVATLKDNSGS
                     KLTNSFQSGNVTLGATNPLTIPLTSSVNVNQSYVYCNFRYSSSMIDRTATCQLSSTGD
                     SVIIEAGAPSTSSYVNWYVVEFSRGALVQRGSGSLTSTEVLKNISLTTSVDLSRTFVI
                     GYSRGSVSNSSIDEQRTLRFRLSSNQNLEVVRNESGSQTYFEWQVIQLDGARVQSGIT
                     SILSGSYLNSSSISSINLNNSFLLINFAAGAGINGVEKNYLSRAYYSNNKQITFERYG
                     NNDSVDISWYAIELIDGTSSQSGNTTLSPTSTSENATINSVDTGRTMIIFSNQVEDGD
                     SPATTQDSGTFSSNFLDTTTIQFSRYNAESNQAIVSWYAIQFQ"
     gene            complement(13987..15060)
                     /locus_tag="PLF66_17805"
     CDS             complement(13987..15060)
                     /locus_tag="PLF66_17805"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HMZ65950.1"
                     /translation="MIKYTLSLGLILLFAFCKEKQTTVENTEKKENLQIVVLVAVGDV
                     TASGKKIKANDIVQENELIKIGKKSFADLQVLNQEITFRAKEQTEITLRNILNSEKTE
                     VAANVKVGSAMFNSGKLKQNSGLNVITPTVVMAVRGTKFETNVSQNGKTETFVLEGKV
                     ATRVNIPELESLTEEDIKKSKTISDLKNTINSKELILEAGQGTVCDSNYPKQIVKDLG
                     LEKSIQNLNSNTIADIDKNVNQDELSKKVSNQKEMLKLNTLSTSEVSKKLKDYEELLA
                     AELSSQNTEERNQKITERNSKINERILKRISEVIGKDPEVLVLKTGEKIKGVVIMDGG
                     KYYVLTPTGKKEYSEEEVENVEF"
     gene            complement(15123..15356)
                     /locus_tag="PLF66_17810"
     CDS             complement(15123..15356)
                     /locus_tag="PLF66_17810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002152925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65951.1"
                     /translation="MKYLESSQIIPMKGMSYTMYEIEGEDKIIRMLTYIPDVEELHIY
                     PKPPVKKLYKPQMCREIDSEQFLSLWKKAEANQ"
     gene            15409..15849
                     /locus_tag="PLF66_17815"
     CDS             15409..15849
                     /locus_tag="PLF66_17815"
                     /inference="COORDINATES: protein motif:HMM:NF024297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HMZ65952.1"
                     /translation="MSLKKLLILSLFVFNFHTLYSQDNSEKKSIEDAIDRYFQTWSNE
                     DMEGYESCFHPDAIIHYERNGEINESKLKRFIEGQKNAHLNSSEKMKEIPLSKKIQID
                     KTIAQVTVRWKLTADNREQYGFDYFTLVKYKKKWKIIYLIFNSD"
     gene            complement(15846..17018)
                     /locus_tag="PLF66_17820"
     CDS             complement(15846..17018)
                     /locus_tag="PLF66_17820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65953.1"
                     /translation="MKKIFIIVVTLYLPTMIFADRIVLKNGKAVVGTVQNQENNMVTI
                     VTTSGERLQIKKDEIVSMIYVETTPKKKPVAPRPVTPAPAKIVSEKKEETSDLAVNST
                     PEKVQVNNNERVVEKTEKVTVDKELTNNVMQKFEDADKRRQQSTDSEIKVLKEELEYL
                     KKERERLQRLNQGDEDFKRTMDKRMASLELRIRRLEKYLAMDETMVDYYQRKRSPWDL
                     VWRSALFPGWGHRYAKEEYTGNTYSTTILVLLGLGYFFNYQANAAEESAKNTLFNNTV
                     VKGYQYSALGIPVTYSNTFLVSSYSNYVSTMSAIDTQKTLSGNLYNAAIALYLIQLVH
                     AYFTGVEWAKVQPRDYSNEGLLKPTSFNIRSKPDTNFYSRVPERGMRYELEFITRF"
     gene            complement(17015..17347)
                     /locus_tag="PLF66_17825"
     CDS             complement(17015..17347)
                     /locus_tag="PLF66_17825"
                     /inference="COORDINATES: protein motif:HMM:NF024546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRL domain-containing protein"
                     /protein_id="HMZ65954.1"
                     /translation="MKKLSLFFILFFSSCITTIAPGILFNNTAEHVYLNKGVSQLGSG
                     RILKMAKSCNYNSIVFAFFYQGKIASVESIAKEEGITKVGVVDYSSFNILGPLYYSNC
                     IIVWGDIE"
     gene            complement(17347..17658)
                     /locus_tag="PLF66_17830"
     CDS             complement(17347..17658)
                     /locus_tag="PLF66_17830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002972204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRL domain-containing protein"
                     /protein_id="HMZ65955.1"
                     /translation="MRMLVILILFSLIGCASGPVGGLLFTNYEYSGEVNPDSSIPLLA
                     EAKGCQFNLLGLFSIGDSSAGQIANNVGIRRIATIDHSTISILHILFVRNCTIITGAT
                     Y"
     gene            complement(17655..18053)
                     /locus_tag="PLF66_17835"
     CDS             complement(17655..18053)
                     /locus_tag="PLF66_17835"
                     /inference="COORDINATES: protein motif:HMM:NF024546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRL domain-containing protein"
                     /protein_id="HMZ65956.1"
                     /translation="MFLSKFIRNRFVFLILPLLLSCTGFNLRTWPLPVSPNTHPIESY
                     AGVDVFFKGGYIYHSGSNVSYTGTGKLEKNGKACSHSFLYLVAFGSSRIYDAKVNGAV
                     NHIGAIEEDVLAILGGFYHRHCTIVIGEGK"
     gene            complement(18176..19150)
                     /locus_tag="PLF66_17840"
     CDS             complement(18176..19150)
                     /locus_tag="PLF66_17840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65957.1"
                     /translation="MLKLFLYSILMTGFLFAETVVEEGPKSKTEIATQPGSVNVTVEH
                     KEIVSEKTEKVIIDKELTDNVIKKFEEADKRRQKATDSELGAFKEELDYLKKEREKLE
                     RSDKGNDEFKRTIDKRIASLEIRMRRIEKYLAMDETMVDYYKRERSPWDLVWRSAVFP
                     GWGHRYAKEEYTGNTYSTTILVLLGIGYFIQYQVGAAKDAANTTLYNNAVVRTLQYSS
                     LGIPANFSNTFLYSSFATYNSAMSAIDSQSQLSSNFINGAIALYFIQLVHVYFTGVEW
                     AKVQPRDYSSEELLKPSSFNFRSKPDNNHYSLTQEKGVRYELEVLQRF"
     gene            complement(19590..21077)
                     /gene="glpK"
                     /locus_tag="PLF66_17845"
     CDS             complement(19590..21077)
                     /gene="glpK"
                     /locus_tag="PLF66_17845"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004783313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HMZ65958.1"
                     /translation="MKSYIIGIDAGTTGIRTFCFDTKGKVISVAYEEFKQIFPKAGWV
                     EHNATEIWQKTEKLILQAIKKGKLNPKNAICIGITNQRETTVLFDKKTGKPVHNAIVW
                     QCRRTADLCMDLKAKGLESEFRNRTGLVLDAYFSGTKIKWILDNVAGARDKANRGDLL
                     FGTIDTWLLYKLTKGKAHKTDHTNASRTLIFNIKDKKWDVDLLNILNIPASILPEAQT
                     SRYEFGKTEGVKGLPDGIPITALVGDQQGALFGQLCTEVGEAKNTYGTGCFLLFNIGN
                     DFQISKNGLITTLACGPEGNTVYALEGSVFIGGAVVQWLRDYMKFFKESKDTFKIVKT
                     LKQEDDIVFVPAFAGLGAPHWDMNARGAIYGITRDTNHAQIIRAALKSIALQSYELVS
                     AMENDTGKSLKILKVDGGATNNEYLMQYQADILGKKVQRPDNVDTTVLGAAYLAGLEC
                     KVFSSVEDLKAMNKKFKEFKPAMKEEVRNREIEKWNTAIVKTKTV"
BASE COUNT         7241 a         3721 c         3187 g         7276 t
ORIGIN      
        1 gttgccctcg gtggattttg aatccactgc gtctaccaat ttcaccacat cggctaattg
       61 attaatctcc agcttccatg tattttccct tcccacgggc gacgatctta ccgatttcat
      121 tttcgatctc ggctctattt tcaataacct tcttattctt tttaacaaac catccacgca
      181 aatggagagg ttcatttata aaagctggtt gaagatatcg aacagttaac tcgccagtca
      241 ttgtctcaaa attcatggct tcattgatct tgatcatgat ttcgtctaga atggtagcta
      301 taattcccgg atgaatagaa tcgggagctc cctgaaactt tgcaggacag gtgtaatcac
      361 cataggctgt cttagtatct tcatcaaatg ttattctaag ctgtaatcca tcattgttat
      421 caggactgga accaaaactt aaattcttct ttgatactaa cttcattaag acaaagagta
      481 cgtgctaaaa tcttttgtca agaaaaattt agttctattc tatcaaaatc catggtttta
      541 agactagtct agaagactgt tcatgtcgaa aatgaaaata gattgcatca aaaagcattt
      601 aaagcttttc tatttaaatt acccggagta tacttcgatt tgctacagga tgaataatgg
      661 cgccgcttaa tacaccttcc agtctgactc taagctatga tgccactgag attttaggtg
      721 agcaggagaa aagtttattc cttcacaaaa gacctctctt aaacgcagat acaatggtaa
      781 aaaaaggaca ttttaaaagt gccttagaaa tctattatag aactgctagc aaaatcacaa
      841 atcaaaacat agtagcaaag attcaaaaaa atatttctga tattcataac tatctaaagt
      901 caagtgactc tgaatcagaa aaagcaatct cggaaaaata ttccgataac agaggcaaag
      961 aatttactga atccctcaaa gaactgactg aaaatctttc agactcatta acagaaaaat
     1021 taaacattct aaaacaaata gaaacattac ctcgaaaaaa atccgaaaat ccattatttg
     1081 cagatagagc aatcgaagag cctccaattg aagaagatga aaatggcatt aagacgttag
     1141 acttaggttc cttagaagca aagccaaaac cgaaagaaca aaaacccaaa gatgaagtag
     1201 aagaaattca ggcaacggta gaatatacta atgatgcgga taatgaaggt ttgccgcagg
     1261 ataaaaagcc aaagcaagcc aagattaaaa taccaaaaga tgatttaatt ggtagtagcg
     1321 ataaaacaga agtttccagc caaacaacgg aaataaaaaa cgaaaaggct gagatcaaaa
     1381 acgaaacgaa tattcaaaat gcaaatctac aagctgaatc attacagatc gattctacta
     1441 aacaacttca atctgaagtt acagaaaaca cctcctctaa aattgaacca gattcatatg
     1501 agaacaaacc tccatctaaa gacgttattc catcttctac taaaaaaaat ccagatattg
     1561 tagaagaagt aaaaatcata aaggaatata tacaaaaaat tgaaagcaca gttgctaggt
     1621 ctgactcaga tgaaatagac caaactagaa ttgaaactgg ctggtctgaa ctagaaaaag
     1681 aacctccaaa aaacaaatca gacaaagatt atgctcccca aatgaagaaa tatgaagttg
     1741 gtagcattga aattccagaa gcaacagaat tttttactgg agattttatt tctagagaag
     1801 caagtgaaat acaaagaaaa gaaaaaactg ataaagatag cactaaagaa gaacccttct
     1861 tctctcctga tatagctaat gatacgaata agcaagcaag aataagcgat agtgggcaga
     1921 ctgcttttaa cattccggca atgccccctc ctcttcctcc aaatatcaat gattggctga
     1981 gcaatttata tttttctaaa gaatgggaaa tgtttaagcc cttaccactc aaggaaagac
     2041 gatcaggaaa ggaaagaaga caaaataaat ccaatcaact accagaagga ataaaaaagg
     2101 atcgtaggtc tggaacagat agaagaaaac agaatgaaaa ccttctacat gcaagagatg
     2161 cttttttaaa agactggcat cagagtcagt atccaccaag ctttccaaat ccatattcag
     2221 caggatcatt tcccgctaat cttggtccgg aatcctctgc atttgtagga gaggcatctt
     2281 ctgaaaaaga tgctcatttt gatccaaacc ttagaacctt agaattccta ccaagggacc
     2341 caggctcgca tgaaaaacta caaacaccgg aagacctagt aaaagtagat ttaccagatc
     2401 cagaagaaat aattttaaaa ccacctatag atcaagatga gtatccacgt gaggaagtaa
     2461 aatctgacat agctgacaca ttaaaaaaat acattccggc aaatgaagag ttaggtttac
     2521 cagagagtga taagggtctt gttaaaattg atctacctaa tcctgtttca ctcaaagcgg
     2581 atacgaatga tttaccgcag cgtgaagtct ctggagaaat tgaagtttcc tctaaagatg
     2641 gtactggaat tgttagtgag gcagttggta aagacagatc tggaatgggt cctgttccct
     2701 acactcctga agcttatgaa attccagaag aattaaaaat tgatttacct gatccagatg
     2761 attttattcg aaccaagact ggacatttaa tagatccatt tgctccggaa gaaacgcctg
     2821 atataaatat aattgatggg gatggaccga aagaaacaga tgaaagttct ccagaaattt
     2881 ccgaaattcc tgagttacca ccaccgcctc cagaaccgga aagaacgata cacggtatac
     2941 tagaacttaa accaccggaa atcgatgatg ctccattttt aacattgacg tatgatttta
     3001 gcaaaatccc gcatccattc aagctatcta aaaattatag cataatggag tattcttatt
     3061 ataaatacaa accgatgctt atgaaagcac aagaatttgc gagaaggaaa atgctaaaaa
     3121 atgcattgaa ttattataga gtaattaaat cgcaaaacat tcctcctgaa ttaaaaaaca
     3181 tgattaatag gaatattacc gatatcacag agtttttaga gaaatttatt atgagtaaag
     3241 gtggctaata tcatttagcc acataaacta tttcaggaat gcttcctctg cttttttaat
     3301 gtttgactta taattttcaa atgcgctgtt aatcgcatct ggcttcaata aatctactcc
     3361 tgctaaattt aagatttcta aggatggctt tgatccacca gactttaaaa attggaaaat
     3421 tttgtctttc gtcttatctt tattttcttt caatgattca gataaagaaa gagaagcaca
     3481 atagctagtt gcatatttat aaacataaaa aggggaataa aaatgaggaa ttcgcatcca
     3541 ctcatgtttg atataatcag atgccaaaga atgctctcca taccattcct gaactaaatt
     3601 gtaatacaat tcatcgagtc gatcgggagt aaatacttct cctgaatccg caatcttggc
     3661 agcttccctt tcaaacgttg caaataaaac ctgtctaaga acagttcctt caaaatttgc
     3721 aactgattca gacaatacag attttgctaa gtccgaatta gagtgcttct ccaaaatata
     3781 atttgttaaa agtgcttcat ttaaagtgct agcaacctca gctacaaaaa tcgtataact
     3841 tccataatga taaggttgat atttgtgact ataataacta tgcatagaat gacctaactc
     3901 atgagctaat gtgtaaacat cgctaatggt tccattccat gtttgtagca tatatggcct
     3961 agaatcatag gttccccaag aaaatgctcc cgacctttta ccttcatttt cagctctgtc
     4021 tatccatcta tctgttgtga gcccttttct tgcgatgttt acatattcat ctcccaaggg
     4081 tcgaattgct tccaatacta agtctcttcc ttcctcccag gtaaatttta atggatttgt
     4141 agctttaaac aaggaaacgt atctatcgta aggagccact ttttcaattc caagaatttt
     4201 ctttctaaga tgcatgcttt gatgcaatga atctaaatta tttctgacac ttgtaattag
     4261 attatcataa agagaaatag ggatatcatc ttgaaacaac ttgctttcaa tgaaactttt
     4321 gtatttgttt atttttgcaa gagtagaacc actcaccata tttccataat aatttgctgt
     4381 tatggtattt ctccatttag atatttcttt ataataagaa ttaaatgtat tctctcttag
     4441 aacacgatct gtcgattgaa gatttaggag caaacgggaa ttagccacta gatggtcttt
     4501 gttttctgaa tcctttgcgg tctcaaactt caaatctgca ttgttccatt tactatgaat
     4561 atcatcgaat acatgaaaca ctacatttag ttgagataaa attgcttctt ccttttcaga
     4621 tagaatatgc tctttaaatc ttattatctc actcagtcta aacttataat ccttaagaat
     4681 atcactttct aaccactttt caatatcagg aatttttagt atttctggtt ctacaaatgc
     4741 aaattgagaa tttagttcag aagagcgaac atcaattcta ccacagtatt catttgcttc
     4801 ggtatccgca atatcttctg atgctctaag attcgcatat acatataaat tttcgatttt
     4861 tctagataat tcgtctctgt attggaaaca ctctaaaagt ttctcaggac tttgatgtaa
     4921 tttgtttttg aaattatctt ctaattcttt cttgagagat tctgtatctg gtaaagtttg
     4981 aaatgttttt tcccaatctt taaaatccaa aaataaatca tctaacagcc aagtttcttc
     5041 ttttgaaaca tcttttcttt tgggtaataa attaacactc atactgaatc ctatacatgt
     5101 attttcttgg gtggaagaaa tttggctact ttttcataag cggaatcttt cccatattct
     5161 aatttaccaa atcctctcac agcacctagt tcaattgctt ctctttggta ttcttcataa
     5221 ttggaaatga gcataacagg tatttgccgt aattcatttt ctttctgcat ttctgatatt
     5281 aggatagttc catctgtata gtcaatgtct aactttcgat tgactaatac taaatcgtat
     5341 gagttttttc gtaactcttt caatgcttca tctgttgaat caatttttat gacttggcaa
     5401 tcaaaactat tttcaaaata attgcaaaga gttgaatgat cgggcacaca ttgccctaca
     5461 cttaaaactt tcttcatcta ttcctccttc ttaaatttta aagaaacaga attcatgcaa
     5521 tatcttagac cagtaggctt tggaccatct gaaaacacat gacctaaatg cccgtcacat
     5581 ctagcacaat gcacttctgt tcttgtcata aaaaaacttt tgtcaaccgt tgtttcaacc
     5641 gcctctgaag agattggttc atagaaacta ggccaaccag ttcctgaatc aaatttagcc
     5701 tttgaatcaa agagtaaatt tccacatcca ttgcaatagt attttccagt gtcataattg
     5761 tgattgtatt cccctgaaaa agcaggttca gttccatgtt ttctgagaac tttaaactct
     5821 tggggagata gttgtgcttt ccattcttct tctgatttga taattttatt catagtaatt
     5881 agacttggaa tgattaaacc ttgactgact ataaaaaatc aattttttta ttttaaaaaa
     5941 atccatttaa aatttacgtt taatagaatt agagattatt tagattttat tttttttaaa
     6001 atagacgata gcataactat agatttctat gtataaatat aaatttattt ttaaaaataa
     6061 tatagattga caattagaaa aagttcaatt taagataaat agaaatttat taaatagttt
     6121 cctctcaatt gtaaatattt aaaagaaaag atttttcaaa agtttaaaca gtttttaatt
     6181 tcgaaattta taaaactaga taacactaca atttgtaagg attctttaaa tacatgatgc
     6241 ataccaaagt aatgattgtt gaagacgaaa cgatagtcgg cctagacatg aagaacacac
     6301 ttgttcaatt gggctatgaa gtcacaggca tatcacattc tggtgaaaaa gcaattaatg
     6361 atgctataaa aaataagcca gatattatac taatggatat tcaattagat ggtcaaatga
     6421 cagggattga cactgcaaaa agaataaagc aaagattcga tgtcccgatc atatacttaa
     6481 cctcatactc tgatgatact acattaaaag aggctttatc tacaactcct tccggctatc
     6541 tactaaaacc atttattcct agagaactac atactagcat acaagcagcc cttcatttaa
     6601 aatcaaaaga taatgatact aaattagcta ggttaaaata tatccagagt aatattctca
     6661 aaaacaaaga tctacaaaat caaattaaag acattagaaa agatccaaat cttacacacg
     6721 aaaataaact agaaagactt ttatttatca aagaattaat tcgcaaaaaa aggaaatttg
     6781 aaaaaaagtt tgaagattta tcctatattc attcttcaat cgaaaatcta attgaagaca
     6841 atctatttac acttgaggta aatattgatt atccactgat tttcccaata aaggaacttg
     6901 catatcaact caactacaca gaagatgaaa tcattcgaat gggaagttta agtattcctt
     6961 tgttgattca tccggtagac attgaatcaa tggaaaaatt tcaggataga ataatagcaa
     7021 atccatctat tcctgttgaa gtagaaatca gaatcaaaaa caaatcggat cattatagat
     7081 gtttttatgt gagaggaatt tttgagaaaa aaagtaatac ttttgggaaa attttatatt
     7141 taacaagaga tattacttgg atgaaaagct tgcaatccga ttcccatact gctcgtgaga
     7201 aattagtagc aataagtaat aatccatatt tcggagtcgc tgttttagat aaaaatgcaa
     7261 agtttctaaa tgtgaattct acaattgaaa acttaactgg ttatacaaaa gaagatttaa
     7321 actggcttca tttctctgat ttcatcggag ccaaacactt agtttctatc tgtgaaaatt
     7381 ttgaaagatt aaagaatggg aaattagata tttaccaagc agaagtttta attcatacag
     7441 ctcatcaagg tgtagtctgg gtctatatcg tattaaatgc aatacgcgat cctgaaaaaa
     7501 acgttgaaaa aatcctgtgc acgttagttg acatctctga atcgaagtta ttttctaagc
     7561 ccaagtatat ttagaatcta tacttcacta tgaatctttc ctttttacta agaattattt
     7621 tttttactgt ccttctcttt acattcccta ttttttcaga tgattggtct gaccttgatg
     7681 aaggtgaaaa aaaagaagtt tatgggagcg agaaaagtaa aaaatataaa atgagcaata
     7741 tttttggaga ggtagaaagt tgggacaaac actatgcagt tcgaatcatc tatctctacg
     7801 attatactga ttatccaaaa tataattcta ctcagtttat tcctttttat tacagattaa
     7861 atagtaaaat tgacaatcga gaaaaacttc gttttataaa ttatacaact gaaattcaaa
     7921 aatcaaaagt agataaatca atccttccct tattctattg gggacacaat aatgatctaa
     7981 aattaaatta ccaacttttc ctacctgtat attacaactc ctcaacaaat aattcaaatt
     8041 taactgagaa taaatttatt ttcattccta tcccctttta ttattcattt gaagataaga
     8101 ataaaaaaaa ctcaacttct gaaaaacatt actctattct acatggaaga gttttacaaa
     8161 agaaaaagaa ttcaaatgat acattagaat ctagcttctg gtttccagga tttccattaa
     8221 tgcgttacac gaaaggacag gatttttatt atcattatat cattccattc tatggctatt
     8281 caagaacaga gaatcttcaa agagaagaaa caactttttt gttttcactc tttccatttt
     8341 actactctgc ttcctcttat ccaattgatg gaaataataa gcattctgaa ttaacgcatt
     8401 ggactttatt ttattataga agtttagagg aagacaaagg cggttttcgc gaatataccc
     8461 ataaaactgt ttggggattt cctgttcttc cagttttata ttattcttcc ttccgccaag
     8521 gagaaggata ctataaacgg atacttacat ttttccattg ggaaaaagat gaaaaagaag
     8581 aattaaattc tctttctttt ttaccatttt ttttctacaa aaaaaatgac tactttagaa
     8641 ttccgatcct attcattaat aaggatttaa gcagtccctc tgcctcttac ggtaaaacct
     8701 ttgttccact tcttctctat tacaatcgat gggatttatc aagtcaaacc actgttttag
     8761 gtccttggat ttatactgaa aacaaattaa aaaacgaatc ttttaaaaca ttatttccaa
     8821 tttactggaa gtctgaaagt gaaaagaaag atatcaccct tatctttcca atctatgcaa
     8881 attataatga caaagaaaat gactatcact ttaatccaat atggtttagc agagcgaatt
     8941 taggaacgtt aaatccatcc ttatctctag gcaaaaaaga agggatatgg tatttagata
     9001 cagactttac atttttttac tatcttgcaa gtctctcttt tagacaaacg attgaaaagc
     9061 caaaattttt acgagatgtt ttaaatcgaa acaaaagcta tgaagaattg gagaaagaaa
     9121 ataaagaaga gaaagttaga ataaaaaaag aatctaaaga ttctagtgat cccaaaatca
     9181 caaaaagaaa aacgattaca agggaagact ctttatcatt tagcggctat tctctattat
     9241 tcggattatt atcatatgaa gctgcggatt ctaaaagaca ttttagatta ctacctctct
     9301 cctggtttag ttgggataaa gaatcagatg ataaagtata tgttgcgccg ctttttttat
     9361 ggtatcagtc tgatttatta gaatacttgg ttttatttcc attttatgga aaacaaaaag
     9421 atgagtcttc cgaaaaaaaa gcatttctac tcaatgttta tatttctgaa ttttacaaag
     9481 ataaaaactg ggttgaaaaa tcaatccttt ggcctctcgc aaatcaatat acatcggaag
     9541 ataaatctgg gcatagaata ttacctttct atatccataa aaattctaag tttggagaat
     9601 catctatcac caataattat acgttagctt cctattatga atcaagaaca agtaaagatt
     9661 cagagcagac aactttattt atttggccaa ctctttttta ttataaaagt tattcttata
     9721 aagaaaatcc agaaaaaccc tatagcaaat cacgcttctg gtttacaccc tttttctttc
     9781 gaagcaaaga taatgattcc tctcatacaa atattttttg gattctagat ttttcatttt
     9841 accaaaagaa attagagaga ggaattctat tcccatttta ttattttgct gatgggaact
     9901 ttggaatatt tcctataagc ctaaacaaga caggtagtta tttttggaca tttacatttt
     9961 ttaattatta tcgttctacc gaggacggtt tttattataa ctttttattc ctattagaat
    10021 ctgaaaaatc taaacgtaat tttgaaacaa atttattatt ttattcaatt caatacaaaa
    10081 agagtgaaac agaattttct ttgaatgggt tatgggggta tggctggaac tttaacaaat
    10141 cgaatgatac ctggacagat ctaaacatat tctcctattt aggcggcgga tatcgtttta
    10201 ttggtgatga atcaatctat aatatatctt tattctatta tagagctacc aatggaagtg
    10261 attatcaaaa ctggttacta gggaattaca ctagaaaatc taaatctggg tttaggaata
    10321 actttttata tttaatagac tatgaaaact tttcgtctga cggttcttat acttgggaca
    10381 ttctatttac aggaattcga taccataatt taaaaaatga aaattatttt caaggtgctt
    10441 atggctacct ttggaatttt cgaaaagaga atgagatttg gagagatgca agttttcttt
    10501 ggttaggtta ctcgaataaa aatagtgaaa ctatctacaa cttcttgcct atttttagat
    10561 cctatgatct aaatgaggaa cactccagga tttatggacc aatgcttctt tatacctctg
    10621 aagaaacagg aaagaatttt catttaggac tactcggtct tggatattgg tacaataaag
    10681 atgagagaaa taaggaagaa gatacctata ttttacttgg cagtctgtat agagagtata
    10741 ctcaaaaaga aagaggtttt agagcaagag ggagcctttg gggttggctc tgggattacc
    10801 aaaaagaaga ggagacttct tttgagaaat tttcaatact aaaacttttt tcatacacaa
    10861 aagaagcgga tggaacaaag aaaattttgg ggatttcttt ttaatcaaat tatgaaatat
    10921 aaaaaataca ttatcccgat tcttttcatt ctaatagagg ttttttgctc aggaggaaat
    10981 tctaagagta ttataataca gcaatcttct aaaccatctt attttttcgg caatcaaaaa
    11041 gaatttacaa gtcctttaaa aataaacaca attcaattgc ctctgaatgt ttcatcagag
    11101 tcgtatttaa actttaaaaa cacagttacg aacaatgatt atccttcctt tctatacata
    11161 gaacaattct taaatgcctt tcaatataat ttaccaatta attcagacaa aaataaactt
    11221 agtttgtata ctgaaacagc caatagtcct tggagtaaaa atagaaaatt acttcaagtt
    11281 tcaataatgg gaaattcaaa aattgaaaag gaaacaaata gtttaccaaa aaatcttata
    11341 tttgcaatag atgtctcagg ctcaatggca gaaccaaata gaatgaatct tgccaaacta
    11401 gctctaaagc gttttgtaaa caacttaaat ccaaaggatt ttgtatctat catcacatac
    11461 tcaacggaag ctgaaacttt aattgaacct actgaagctc agcagaaatc aattctatta
    11521 aataaaatag caggaatacg tgatactgga ggaactaacg cttggcctgg aatccaattg
    11581 gcatatagag ttgctaggga aatgcaattc aataaaagac agactaaaat agtaatttta
    11641 agtgatggag actttggtgg tgtaaacgat gaggaatttt tttctcttgt aagaaaagaa
    11701 agattcaaca acacaacaat ttccattctg ggtttttcaa cagaaagaaa aatcaataca
    11761 atggataaat taaatgattt aaaacttggg gattctattt atattgattc aattgaggat
    11821 gctagattaa agctaaatga taattggtta caattttcaa gcatagcgac tgacttaaaa
    11881 ttagaagtaa aatttgactc aaaaaatgta gaatcattta aactaatagg ttttgatcga
    11941 tataatccat ctaaaaataa agaaatcgtg gatgccgatt cctctttttt ttatacagct
    12001 ctttatgaaa tcatcccctc caaagaatcc actcccacag gagagttagc tatatttcgg
    12061 ttatcgtata atacatttaa taaaacagag gaattaaaga gaacagcaat aaattcaaat
    12121 cattcgattt gggactcaag cgacagccta agactagcag caggaatttc cgctttaggt
    12181 atggttgtaa atgattcagt tacctctgaa aatattgatt tttatactgc catacaccta
    12241 attcaatcta ccttaattta tgatccggat aaatcgaggc aagaattatt aaatttagtt
    12301 ataaaactaa aaaatatgaa atagatcttt agatgctatt tattgaaatt gaatcgcata
    12361 ccaactaacg atagcttgat tcgattccgc attatatcta gaaaattgaa ttgtcgttgt
    12421 atctagaaaa ttactagaaa atgttcccga gtcttgcgtt gttgcaggcg aatcaccatc
    12481 ttcgacttga ttagaaaaaa tgatcattgt gcgaccagta tcaacactat ttatagttgc
    12541 attttcagat gtagatgttg gggataatgt cgtattaccg ctttgagaac ttgttccatc
    12601 aattagttca atcgcatacc aagaaatgtc aaccgaatca ttatttccat atctttcaaa
    12661 tgtaatctgt ttattgttgg aatagtaagc tctagataga taattttttt ctaccccatt
    12721 gattcctgct cccgctgcga agttgatcaa taggaatgaa ttatttaaat tgattgaact
    12781 gatactagaa ctatttaaat aggaaccaga cagaatacta gttataccag actgaactct
    12841 tgccccatct aactgaatca cttgccactc aaaatatgtt tgactaccag attcattacg
    12901 aaccacttca agattttgat ttgacgatag ccgaaatctc aaagtcctct gttcatcaat
    12961 agaagaattg gaaacactcc ccctgctata tccaatcaca aatgttctac ttaaatcgac
    13021 actggttgtt aaacttatat tttttaaaac ttctgttgaa gtaagtgatc cacttcctct
    13081 ttgcactaat gcacctcgtg aaaattcaac cacataccaa tttacataag aactagtaga
    13141 gggtgctcct gcttcaataa tgactgagtc tccagtagaa gaaagctgac aggttgccgt
    13201 tctatcaatc atggaagaac tatatctaaa attgcaatat acataagatt gatttacatt
    13261 cactgaagag gttaatggaa tcgttagagg attagtggct cctagagtaa catttccaga
    13321 ttgaaatgaa tttgtcaatt tcgatcctga attatctttt aaagtagcta ccacgattcc
    13381 aatcacgact ctttgttgaa aaggattact tgttttaatt gcatgaatga tatttacttt
    13441 ttgagatcct tcctctatat tatcgtataa cgcatataca gttacagttt gtggctgatt
    13501 ccaattctct ggtgtaaagt taatatttgt gagaccagta gtattatcgt ttacttttat
    13561 ttgtgtagaa tcaaacccta tatcgatgga tataggcata ttaggatttg aatttaagac
    13621 aatggtataa gtatcggttg tagttccctc aaataaatca gtagatccat ctgattcagt
    13681 aatggtagcc gagacattga cattagaaac tggtatagaa tattgtaagg aaggcttttt
    13741 tttaaattca caaaatatat ttataaaaaa cactaagatt ataaatatat ataaaaattc
    13801 ttttttaaaa tatgataaca tttctattaa cgcttttttt tctaatgttt ttttatttgt
    13861 aatagtaggt tttttatgga gaagtttgag aaggactgat actagatcat cttatcagtc
    13921 cttattgaaa gattttactt tgaaaatttc atttaatctt tatttcagga tgttaccttc
    13981 taaaaattaa aattccacat tttctacttc ctcttctgaa tattcctttt tccctgttgg
    14041 agttagaacg tagtatttgc ctccatccat aatgaccacg ccttttattt tttctccagt
    14101 tttcaaaact agaacttccg ggtctttacc aatcacttcc gaaattcttt ttagtattct
    14161 ttcatttatt ttgctatttc tttctgtaat tttctgattt ctctcttctg tattttggga
    14221 agataactca gctgcaagta actcctcata atcttttaat tttttagaaa cctcagaagt
    14281 tgatagagtg tttaatttaa gcatctcctt ttggtttgat actttttttg ataattcatc
    14341 ttggtttaca tttttgtcta tgtcagcgat tgtattgcta tttaaattct gaatcgattt
    14401 ttctaaacct aaatctttta caatttgttt aggataatta gaatcacaaa ctgttccctg
    14461 acctgcttct aaaataagct cttttgaatt gattgtattt tttaaatcac taattgtttt
    14521 acttttttta atatcttcct cagttaaaga ttctaactct gggatgttga ctcttgtagc
    14581 aactttacct tctaagacaa aggtctctgt ctttccattt tgagatacat tcgtttcaaa
    14641 ctttgttccc cttactgcca taaccactgt cggagttatt acattcagtc cggaattttg
    14701 ttttaattta cctgaattaa acattgctga gcctactttt acgtttgccg ccacttcagt
    14761 tttttccgaa tttaaaatat ttctgagggt aatctctgtt tgctccttcg ctctaaaagt
    14821 aatctcctga tttaaaactt gcaaatcagc aaaagatttt ttaccaattt taattagttc
    14881 attttcttga actatatcgt tcgctttaat tttttttccg gaagcagtta catccccaac
    14941 tgcaaccaat acaactattt gtagattttc ttttttctcg gtattttcaa ctgttgtttg
    15001 cttttcctta caaaaagcaa aaagtaaaat taaacctaaa ctcaatgtat actttatcat
    15061 atcaatctcc cttaaaacaa gactttgaat ctttctttta tttgtcaatt cgttgttata
    15121 gcttactgat ttgcttctgc tttcttccat aaacttaaaa attgttcaga atctatttct
    15181 ctacacattt gcggtttgta taattttttg actggaggct ttggataaat atgtagttct
    15241 tctacatctg gaatgtaggt tagcattcta ataattttat cttcgccttc aatttcatac
    15301 atggtatacg acataccttt catcgggata atttgtgagg attctaagta tttcataatt
    15361 aaatcttttt tgatttgacc tatttgtaaa tcaaattaac gttttggtat gtctttaaaa
    15421 aaacttttaa tactctcttt gttcgtattc aactttcata ctctatactc acaggataat
    15481 tcagagaaaa aaagtatcga agatgcgatc gataggtatt ttcaaacttg gtcgaatgaa
    15541 gatatggagg gctacgaatc ttgctttcat cctgatgcaa tcattcatta tgagcgcaat
    15601 ggggaaatca atgagagtaa gttaaaacga ttcattgagg gacaaaaaaa tgcgcattta
    15661 aatagttctg agaaaatgaa agaaatacca ctttcaaaga agattcagat tgataaaact
    15721 atcgcccaag taactgttcg ctggaagtta acagcggata accgcgaaca gtatggcttt
    15781 gactatttca ctcttgtaaa atacaaaaag aaatggaaaa ttatctattt gatttttaac
    15841 tcagattaaa aacgagttat aaactccaat tcatatctca ttcctctttc aggaactctt
    15901 gaatagaaat ttgtgtctgg ctttgagcgt atattaaaac ttgtcggttt taagagtcct
    15961 tcattggagt agtctctcgg ctgaactttc gcccattcaa ctcccgtaaa ataagcatga
    16021 actaactgaa ttaaatacaa ggcaatagcc gcattgtata aattaccaga aagagttttt
    16081 tgtgtatcga tagctgacat agtagaaaca taattcgaat agctgcttac caaaaaagta
    16141 tttgaatatg taactggaat tccaagagca ctgtattggt agccttttac tactgtattg
    16201 ttaaaaagag tattcttagc tgactcttcc gccgcattgg cttgataatt aaaaaaatat
    16261 cctaaaccta ataagacaag aatcgtggtt gaataggtat tccctgtgta ttcctctttc
    16321 gcatatcgat gtccccatcc aggaaaaagg gctgaacgcc aaactaaatc ccatggactt
    16381 ctttttcttt ggtagtaatc taccatagtt tcatccattg ctagatattt ttctaaacga
    16441 cgaatcctta gttctaaaga agccattcgc ttatccatag ttctcttaaa gtcttcgtct
    16501 ccttgattca atctctgtaa tcgctctctt tcttttttta aatattctaa ctcttctttt
    16561 agaactttta tttctgaatc agttgattgt tgtcttcttt tatctgcatc ctcgaatttt
    16621 tgcatgacat tgtttgtaag ttctttgtct acagtaactt tttccgtttt ttccacaact
    16681 cgctcattat tattcacctg cactttctca ggagttgaat tcaccgctag atccgaagtt
    16741 tcttctttct tttctgaaac aatctttgca ggagcaggag tgactggtct aggtgcaacc
    16801 ggcttttttt tgggtgtagt ctctacatat atcatagaaa caatttcatc tttcttgatt
    16861 tgtagccttt ctccacttgt tgtgacaatt gtcaccatat tgttttcttg gttttgaact
    16921 gttcctacaa cagccttacc atttttcaaa acaattctat ctgcaaaaat cattgtaggc
    16981 aaatagagag taaccacaat tataaatatt tttttcattc tatatctccc catactataa
    17041 tacaatttga ataatataat ggtcctagta tattaaaaga ggaatagtca acaactccca
    17101 cttttgttat gccttcttct ttggctatgc tctctacact tgctatttta ccttgataga
    17161 aaaaagcaaa tacaattgaa ttgtaattac aagactttgc catctttaaa atacgaccac
    17221 ttcctaactg actaacacct ttattcaaat aaacatgttc agcagtgtta ttgaaaagta
    17281 tacctggggc aattgtagtg atacaggaac taaaaaatag aataaaaaat aaacttagtt
    17341 ttttcattaa taggttgctc ctgttatgat tgtgcaattt ctaacaaata atatatgtag
    17401 aatggatata gtagaatgat caatcgttgc gattctacga attccaacgt tattagctat
    17461 ctgtccagca ctcgaatcac caatcgaaaa taaaccaagt agattaaact gacatccctt
    17521 tgcttcggct aacagtggta tactactatc tggatttact tctccagaat attcatagtt
    17581 tgtaaataat agacccccaa ctggtccaga tgcacatcca atcagactaa ataaaattag
    17641 aatcacaagc attctcattt tccctctcct atcacaatgg tgcaatgtct atgataaaaa
    17701 ccgccaagaa ttgcaagcac gtcttcttct attgctccta tatgattcac cgctccattt
    17761 actttggcgt cataaatcct agaactgccg aaggcaacta gatacaaaaa tgaatgggag
    17821 caagcttttc catttttttc taattttccg gttccagtat aggacacatt agaaccacta
    17881 tgataaatat aaccaccctt aaaaaataca tcaacacctg cataagattc tattggatgt
    17941 gtgttaggcg aaacaggaag aggccaggtc ctcaaattga atccagtgca acttagcagg
    18001 agaggtaaaa tcagaaaaac aaatctattt cttataaatt tggataaaaa cataaaaaac
    18061 accttttaat ttcaaattat aactgtaaat gcttttttat tttagaataa ataacaaaac
    18121 tgaaaagatt ctttttttat aaattgatct tgctatttaa ttttaacctc ataaattaaa
    18181 acctttgtaa cacttccaat tcataacgaa ctcctttttc ttgagtgagg gaataatggt
    18241 tgttatctgg cttagagcga aaattaaaac tagaaggttt tagaagttct tcactggaat
    18301 agtctcttgg ttgaactttt gcccactcaa cacctgtaaa atatacatga actaattgga
    18361 taaaataaag agcaatagca ccatttataa agttgctcga caattgggat tgggaatcga
    18421 tagctgacat agcagaatta taagtagcaa aactactgta taaaaatgta ttagaaaaat
    18481 tggcagggat accaagactg ctgtattgaa gtgttcggac cactgcattg ttataaagtg
    18541 tagtatttgc tgcatctttt gctgcaccta cttggtattg aataaaatat ccaataccga
    18601 gtaaaactaa gatagtagta gaataagtat tcccggtata ttcttctttt gcatatcgat
    18661 gaccccaacc aggaaacaca gctgatctcc aaactaaatc ccaggggctt ctttctcttt
    18721 tataataatc taccatagtt tcatccatag ctagatattt ttctattctt ctcattctta
    18781 tttcaagaga agcaattctc ttatcaatag tccttttaaa ttcatcattt cctttgtctg
    18841 acctttcgag cttctctctt tcttttttta aataatctag ctcttcttta aaagctccta
    18901 actctgaatc tgtagctttt tgccttcttt tatcggcttc ctcaaatttt ttaattacgt
    18961 tatccgtaag ctctttatca atgattactt tttctgtttt ttcactaaca atctctttat
    19021 gctccacggt aacattgaca cttcctggct gcgttgcaat ttctgtttta cttttgggtc
    19081 cctcttccac aaccgtctca gcaaataaaa aaccagtcat tagaatacta tataagaata
    19141 gttttaacat ttttatatcc tttcttttgt atttgtttaa tagctttaaa aaaattccct
    19201 actcatcatt aaagctcgaa tctaaaagtt attcgcatct agaattcagc actaatttaa
    19261 gaatttcaat ttagaatgaa agatttattt aaacatgaaa tccgttctta agaattagtt
    19321 atcaaatcaa ttaattctta ttctttaagg tttgattttt ataaaattct aattttattt
    19381 taaagattca aaaaacttaa aggatacttt tagaaatgct tttttaaaga gcaaatattt
    19441 tttatgaatt atattgtttt tttatttatt tttgcggcta attgacatta aacacgtaat
    19501 tttttacgta gtttgtcaaa gcatatcgtt tgcaattcga atctgaataa aaaccgaatg
    19561 actttacaaa agtcattcgg tttataaaat tatactgtct tagttttgac gatcgcagta
    19621 ttccattttt cgatttctct gtttctaact tcttctttca tagccggttt aaattctttg
    19681 aattttttat tcattgcttt caaatcttct actgatgaga aaactttgca ttcaagtcct
    19741 gctaagtaag ctgcacctaa aacagttgta tctacattat caggtctttg gactttttta
    19801 ccgagaatat ccgcttggta ttgcattaaa tattcattgt tggttgcacc accatctact
    19861 ttcaaaatct taagagactt tccagtatcg ttctccatag cagataccaa ttcatatgat
    19921 tgtagagcaa ttgacttaag agctgctctt ataatctgtg catgatttgt atctcgagta
    19981 attccgtata tcgctcctct tgcattcata tcccaatgag gtgccccaag acctgcaaag
    20041 gctggaacaa atactatatc atcttcctgt tttagagttt tcacaatttt aaatgtatct
    20101 tttgactctt tgaaaaattt catataatca cgaagccatt gcacaacagc tccaccaata
    20161 aatacagaac cttcaagagc ataaacggta ttaccctcag gaccacaagc aagagttgta
    20221 attaaaccat ttttagaaat ttgaaaatca tttcctatat taaataatag aaaacatcct
    20281 gttccatacg tattctttgc ttctccaact tcagtgcaaa gctgtccaaa gagagctcct
    20341 tgctgatctc ctactaacgc tgtgattggg attccatctg gaagtccttt tacaccttct
    20401 gtctttccaa attcatagcg agaggtttgc gcttctggaa gaattgaagc tgggatattt
    20461 aaaatattta ataaatctac atcccatttt ttatctttaa tgttgaaaat aagtgttcta
    20521 gatgcgttag tatgatcagt cttgtgagcc tttccttttg ttaatttata taagagccaa
    20581 gtgtcaatcg ttccgaaaag aagatctccg cgatttgctt tatcccttgc tccagctaca
    20641 ttgtctaaaa tccattttat ttttgtccca ctgaaatatg catctaatac aagtcctgtt
    20701 ctatttctaa actcagactc taaacctttt gcttttaaat ccatacataa atcagcagtt
    20761 cttctacatt gccatacaat agcattgtga actggttttc ctgtcttttt gtcaaaaagc
    20821 actgtagttt ctctttggtt tgtaattccg atacagatag catttttagg gtttagtttt
    20881 ccttttttaa ttgcttgcaa aattaacttc tcggtctttt gccagatctc agttgcgttg
    20941 tgctctaccc agcctgcttt tggaaaaatt tgtttaaatt cttcataggc aactgaaatt
    21001 acttttcctt tcgtatcaaa gcaaaatgtt ctgattccag ttgttcctgc atcaattcct
    21061 attatatagc tcttcacttc aaactccttc ctcgaatttc ttttgaaatg aatctatgta
    21121 aggaagctat tgtaaagaat aaaaatacac tacattttaa aaaacttggc aagggacttt
    21181 cttaaataat cgtaagttgt ttgttcgaat ttgaaaactt tcgatccgct tttcttctcc
    21241 catagcgaaa gaaatattta aatttgaatg gatcaaactt ttcaaagagg ttttactcca
    21301 ttttccattg aactctccga aagctaagct attctgtaag tgattgatta tattgtttta
    21361 ttagaattcc cgtaacctat caggctgcta agttactttt atcaagagaa tataatttta
    21421 atata
//
LOCUS       DAOQOS010000091        21424 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_1186_length_21424_cov_6.688989, whole genome shotgun sequence.
ACCESSION   DAOQOS010000091 DAOQOS010000000
VERSION     DAOQOS010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 21424)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21424)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21424
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1186_length_21424_cov_6.688989"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..259
                     /locus_tag="PLF66_17850"
     CDS             <1..259
                     /locus_tag="PLF66_17850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65959.1"
                     /translation="RTLCFLPLIFYFITLIGYRFSNGSINKRVWAAVQKRINHEEHRE
                     HEEKRRFIIYSLRESSCASWLKIRKLTALADRLPFSHLTRS"
     gene            344..679
                     /locus_tag="PLF66_17855"
     CDS             344..679
                     /locus_tag="PLF66_17855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010577132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HMZ65960.1"
                     /translation="MNVFSALADTTRRDIVVLLARKGELSMSDISKNFTMTPPAISQH
                     LKILKEAKVIQVKKEAQLRLYSIDQSGLDEMDEWISEVRNLWTKRFNSLGNYLEKLKK
                     ERGIGKKSR"
     gene            660..1154
                     /locus_tag="PLF66_17860"
     CDS             660..1154
                     /locus_tag="PLF66_17860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004476461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HMZ65961.1"
                     /translation="MEKNLVKTIRKDNKVSFQIFFDSPLDLVFEVWSSSEHLGQWWGP
                     DGFTITTSQFDFSNGGIWNFVMHGPDGTDYSNQIEFIDISFQKHIFYKHKGTGETNSG
                     IHFETKVFFEKLENGTLVKTEMEFPSKETLDLVADKFGAIEGGQEHLKNLGKYIESII
                     RRDV"
     gene            1151..1816
                     /locus_tag="PLF66_17865"
     CDS             1151..1816
                     /locus_tag="PLF66_17865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004784199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HMZ65962.1"
                     /translation="MSKLKVLCFGVSTDGYGAGPNQSLENPMGEGGMALHEWVFHTNE
                     FQKMHGEMEGGIPKVSGSFGIDNDFAKRGFENIGSWILGRYMFGPSRGPWKKEDDWKG
                     WWGPNPPYHTSVYILTHHPKDPIVMEGGTTFYFVTEGIEKALELAKKAANGKDVRLGG
                     GANTVRQYLKAGLVDELHLAIAPALLGSGETLLKGINLPELGYRITEFVNSEKATHVV
                     FKK"
     gene            complement(1926..2882)
                     /locus_tag="PLF66_17870"
     CDS             complement(1926..2882)
                     /locus_tag="PLF66_17870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HMZ65963.1"
                     /translation="MKIKTINQIILIVMLSFLFASCGKKTISQETPDTQAKEIIQQIQ
                     NAKKEILAYKENGKKLCETPEDCLFLSFWDFDGTILKGDCSEGLIENGQVVYKGLEQV
                     SIEAGLSPKYDKNGYEKFVKAEERLDKTKGHIVAYDYIAQIFAGRDRKEMERFSTNHF
                     ESVLQNYYFSQSIEIFNSMREQSDFRVYIISASPDFFVKGSSTTVNVPLTQIRGVNLE
                     DNNGILTEKVILPITYAEGKTKTVLKIVDEIKAEGKYKNIFVLAGFGNSYHTDGNFLK
                     YISTQKLVAGKPIAVMINGGQTPAEYEGFFYKVSHSKTLSGK"
     gene            3013..3480
                     /locus_tag="PLF66_17875"
     CDS             3013..3480
                     /locus_tag="PLF66_17875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002976921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65964.1"
                     /translation="MTLYLLFLIIHIFSAMFWIGGTLFYVFIIFKVLTKEDMKPFKSL
                     VIKESALQFRNISYVIFMTLFLSGLGLIFKKHNWSDLIHGLISVKLVLFILLLISSIY
                     HDFVTGPKTFLFSETDLIKYNQYRKISSYFGRINLLISISIAILGVLISRGIK"
     gene            3477..3845
                     /locus_tag="PLF66_17880"
     CDS             3477..3845
                     /locus_tag="PLF66_17880"
                     /inference="COORDINATES: protein motif:HMM:NF022443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2809 domain-containing protein"
                     /protein_id="HMZ65965.1"
                     /translation="MTHRKRYLTLSIISFLICVLIVLLFSNHGFIRGFLGDLIIVIFL
                     FFSIKLIYPFSNFALSLSVVIFAYCIEFTQYIKIIPLLGLKENIFTKIVFGSVFDPFD
                     LLAYTVGGVISYRIGNKIEN"
     gene            4003..6051
                     /locus_tag="PLF66_17885"
     CDS             4003..6051
                     /locus_tag="PLF66_17885"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HMZ65966.1"
                     /translation="MKTIPNSSLHNLKRRILLFLLFFLITPIHGENIIFDLADKKDYP
                     LILTSHATVLEDSSLDLKIEDILSPKHNSEFKLRNISGNAYNFSYTDSAIWIKLSIRN
                     SEPITKDYFLEIQYPRLMNIFLYLPEGNSYKKVNSGYSVPQDERPYKSRYFTFPIRLD
                     ANKTSSIYLRITSPNAIVLPFFLWDTTSYHNHDVEDHIIQSLYFGILFSMIAYNLLIY
                     IFLRDKSYLYYVLFVISSGVGIAAFNGIAGEWLWGDNPYIEKIGVNAWSVIACTTLMF
                     FMRRMLNSKIDTPILDKFNWLTMFIQWTALFLMLWDFKGFVKLVIFSQIFIAGWITFY
                     SIIRSFQKNRASYFFVTAFTFLMIAISSGYLRVLGILPINKFTTEGVQISSAIQMILL
                     AIALADRYNTLRKEKERAEALIQENLKKSNLELEEKVKERTHELNKSLHLIKEDLNLA
                     KKIQMNALSMNPTDIKDLKLAIKYLPMTEVGGDFFDISKIDDRTYRIFIADATGHGVQ
                     AAMITMVIKGIYDNIKSFGLKPNEILEIFNNEFIHKYISLNSYLTAIVVDIDTESKTL
                     TYVSAGHPSSILLSRGEIKELTRTGKIIGVFKGTNYQSITINFSEKDKLFLFTDGIFE
                     EFNKSEEEFGEERLQEIIRKNHDKSISEIIDISLDELNSFLGAKEKQDDITIIGIELN
                     "
     gene            complement(6259..6453)
                     /locus_tag="PLF66_17890"
     CDS             complement(6259..6453)
                     /locus_tag="PLF66_17890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65967.1"
                     /translation="MNLLLHSLLQICIAILDLVPMLILFKKFILGSGDKGFIILEFST
                     KSYTSLKPEIIMNQILDLEK"
     gene            6650..7456
                     /locus_tag="PLF66_17895"
     CDS             6650..7456
                     /locus_tag="PLF66_17895"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HMZ65968.1"
                     /translation="MRALILENSKLVRNMIRAIFESDKFEVFEAGTSSEANQNYAQET
                     FDVITLSLYLEGEDGFDICRRIRNAKEGEAFYNSKSAVIIFITSNDTIESRLKGFSVG
                     ATEFVLKKNLEEGLKPIISNLINPKGNLENTKILIVDDSYINRLMILQVLKNKGAELI
                     EADNGKEALNLIKERDNKIDLILTDYYMPEMNGTDLCLEIRKIYFLKYLPVIILTGAD
                     DKSEVLSIFQAGATDYIIKPFIKEELLARIENHLEAWLYRREILSKLHRI"
     gene            complement(7613..9199)
                     /locus_tag="PLF66_17900"
     CDS             complement(7613..9199)
                     /locus_tag="PLF66_17900"
                     /inference="COORDINATES: protein motif:HMM:NF013086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HMZ65969.1"
                     /translation="MSNVGKKYNTEDASKKYDAIVIGSGMGGLTTAAILSKAGKAVLV
                     LEQHYTAGGFTHSYSRKGYEWDVGLHYIGDVHKEWSPLRKIYDWISDGELKWSYMDPV
                     YDKIFIQENRYDFRAGRENFVTELLKHFPNAKTSLEKYLSLLDEVNLLAMPAIVTQKL
                     TPSFLSGISGLITQPILKKYFGRTTFEVLSEITDDPKLISVLSSQWGDYGLPPKQSSF
                     AMHAVVAHHYLDGGNFPVGGASSIARTIEKVIQKSNGKILVGKEVSEILIKNNKAYGV
                     KMENGDEILAPIVISATGYINTYRKLIAKDIASDLGLLSNLKKIKPSVGHICLYTGIK
                     ASKKDLKLENGNLWIYPDYDHDKTYSKYLQNQNSDFPLVYISFPSFKDPEWDLHYEGK
                     STIDVIAPAAYEWFQKWKDKPWRKRGEDYNELKEKFSERLLDYVYKYVPEVKGKVDFY
                     ELSTPLSTEYFNKYQHGELYGLDHTPDRFAEKWMRPKSPIDSLYISGQDILFAGVASA
                     LASGFMTATEILGVNLIKAL"
     gene            9401..9640
                     /locus_tag="PLF66_17905"
     CDS             9401..9640
                     /locus_tag="PLF66_17905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65970.1"
                     /translation="MKRFILFMIVLKMIGCHSVKDTFIENVSSEFKEQPIQEKEILTE
                     KDLNGLPTLVQKYYDTLALLVRKKFKTLDWNSTQK"
     gene            9637..10227
                     /locus_tag="PLF66_17910"
     CDS             9637..10227
                     /locus_tag="PLF66_17910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65971.1"
                     /translation="MRKTNESAPMKSDTIQYNFYTNPARHFYLETSMFLIPARVLHSY
                     SKEKASMQVRIANLFNITNISGEELSKTETLTLLNDLCFFAPAALIDKRIQWETIDDT
                     SVKAIFQNGRHKVTAVLFFNELGELINFTTDERGALQDDNTMKYVKWSTPIGNYKDFN
                     GRKIATYGEAIYHYPEGDLTYGKFFLRKIDYNVKEF"
     gene            10211..10663
                     /locus_tag="PLF66_17915"
     CDS             10211..10663
                     /locus_tag="PLF66_17915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65972.1"
                     /translation="MSKNSRLINTRDAFGLLFLVSNAIEIIADKHLSPYKITTKQFFL
                     SLILIQNQKAALTISKAASLLGTSRQNTKQLALKLEKIGFCKIIPNENDKRILNIEIT
                     SKNIDFWKRIDKENQAFLKKTFENLSDQDISSLKEGLIKLYNQIQEEK"
     gene            10665..11828
                     /locus_tag="PLF66_17920"
     CDS             10665..11828
                     /locus_tag="PLF66_17920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65973.1"
                     /translation="MTISRKDFLDKTTHLGLLLTTSQLLQYCSSLPDRYADTSPFDQE
                     NLSPLWKSIYAGIGAPNPHNTQAWKIKILNENEILLFVDESRILLDTDPPARQIHIGQ
                     GSFIECFSIGAAQLGYTTKVQYFPQGEYKTSEIGKKPVAKLTLSAGDSNKKKFFKSLN
                     IRNTNRSIYSGDFSETDRDKIQNEIQYDKSNFKWILSSDSRFAKIQDLLVEAFAIETN
                     LKRTNEENRIWFRVTDKEIYSKRDGISLRGNGMSGLSYKIISNFFVSTKPEDWHSDFT
                     RKAGIDSFKEQVKSSKAFIYILTSKNEMKDWVESGEKYAHLNIAVNSLSYSMHPMSQF
                     LQEYPEMEKVKLEFEKLMEIPPKAKIQMFARIGKSDYFFQSPRRNPQDMIVKS"
     gene            11877..13073
                     /locus_tag="PLF66_17925"
     CDS             11877..13073
                     /locus_tag="PLF66_17925"
                     /inference="COORDINATES: protein motif:HMM:NF022853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HMZ65974.1"
                     /translation="MTRILLFLLLISSVFGEETPYSKVKITPKNALVLRTGGESDFEV
                     NINLPHKHYLYLSHTNKDAIGILTNFSFPIESGFQLIETKRPKGVKKFDEMILRESGT
                     FSFKAFELGLRKNGSNAKIPMSIRIQICEEKENPVCYPPETLIKEVIAEVRDDKRIIN
                     TREIESISWETDFENAITKAKASNLNIYGIITEPSWCGACRYMEQEAFSKPEMKKVLK
                     EKFIPWKIKDEEYNKVPLGNGTFGIPTYFVLNPNGSSLAKWTGARDSKSLLTLLKPYE
                     KSNPSPELEPSPPSPDSSEFEVKGNDESKCTLLIGKDYNLIAQKSGEFLNDGKIRFGF
                     NNQELKIKQFSRNVSNRKAYPVKFTTLGLRIEKPEIKEYWVLECKNSILVGKIEGSQI
                     EFSIEK"
     gene            complement(13363..14352)
                     /locus_tag="PLF66_17930"
     CDS             complement(13363..14352)
                     /locus_tag="PLF66_17930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65975.1"
                     /translation="MLKSNSDLQKCFLDTTIYQVCQGNCRSVLSINSAWMGNFKKFCI
                     AGNKLNSFIKGENTFTVAASAFQQAQAAVIDEFANESSDKLDLIRYFFGICEPEEVQE
                     LVEKLSNDTLFRILQIDYENYLKVRKMLAKKPDVNNYFALKSSRYWKLVSNQRICNMI
                     VFLVREKQEYKLASQFLLILPPEVMSEFDKYTDLSEDDERNLYLALEDNIYNLPLISP
                     KIYDHMLNLFRENMEIFFILETMGELVKRRGQIDDITSSFIKHLKKSGQRLTIQWIYS
                     ELYGLEYELVVEVLDQLREKDYISSSEKTTLQSLLKSGSLDYLKEMKMEILNN"
     gene            complement(14357..14815)
                     /locus_tag="PLF66_17935"
     CDS             complement(14357..14815)
                     /locus_tag="PLF66_17935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65976.1"
                     /translation="MRYIFLLLLFVFSCKGQIDSGIKNSLLKVLSENQKVHEILITKE
                     NLIPDTKALLESVQQAKIASEKNESIKKQIEKMESTLTNINTKDQEAFFNGLSSFSET
                     LTEIIKANQIQTEYNKFYCPMVAKYWVAKGEMVQNPYAPDMRECGELVKE"
     gene            14994..15833
                     /locus_tag="PLF66_17940"
     CDS             14994..15833
                     /locus_tag="PLF66_17940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65977.1"
                     /translation="MRNIRYLKLKLIALSLFTFLSSTILAETVILRTHDVIHGKITFQ
                     DANVLRMNDDLGKPLEIQKSEILKVSYRDVKDAKEIKRIIEEEESKLPPDQRKGAKSA
                     SKKRTFDIVWRSALIPGWGLWKANKKWLAALVFVGVAGVGAYSVSKVNAFDSAGKTYE
                     SNSLLIGLYVLRQTQAGASSNQTVNLGTGIILGQAAFAPYGQAQTNGNNAIQALGFVY
                     AVQLIHSIFVGRDYAKEEPAKGIGTKTAYGDINFNATPKMALTPSGLNRETYYELNYS
                     LDF"
     gene            16404..19730
                     /locus_tag="PLF66_17945"
     CDS             16404..19730
                     /locus_tag="PLF66_17945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010411574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HMZ65978.1"
                     /translation="MSIRVALHHKTHYQYNKPITIFPQVVRLRPAPHTRTPVEAYSLK
                     IQPGNHFINWMQDPFGNYEARLVFPEKASSLTVEIDLVANMTVINPFDFFLDESADEF
                     PFEYSDDLKGELLPYLQIKEDGALLKKLISKLDLKKRRTVDFLVYINQVVQSEVGYII
                     RMEPGVQTPEETLTIKKGSCRDSAWLLVQMLRHIGLAARFTSGYLIQLVPDVKSLDGP
                     SGTNVDFTDLHAWTEVYLPGAGWVGLDPTSGLFAGEGHIPLACTPNPASAAPIAGGIQ
                     MGAEAKLQFEMSVTRINEEPRVTKPYTEEEWKKIYELGHKVDIDLKRNDVRLTMGGEP
                     TFVSIDDMDGAEWNVEALGPTKKRYAIELFNRLKEKYASGPLLHYGQGKWYPGESLPR
                     WALSCYWRKDGIAIWNDPKLIADERLTGTYTKEDAEKFIRRLTTHLAITDEHVVPGFE
                     DVYYYLWKEGTLPVNVDPFLKDLKDSEERKTLRRVLEAGLGTTVGFVLPIKWDWQSRV
                     WRSGRWHLKRERLYLLPGDSPMGYRLPLNSLPHSGNYYYDEEPSSFVKPKPLENIREQ
                     VAKRYQAFVNKGSSSGSGNSELDSLYKKHSANRELELNDDSWEMIRTALCVEPRNGNL
                     YIFIPPMYHIEEYLDLIASIEMTAADLGMPVLIEGYLPPHDIRLQKFQITPDPGVIEV
                     NVHPSDNWETLVRNTEILYDEARYSRLGTEKFMLDGRHTGTGGGNHITLGGNTPSDSP
                     LLRRPDLLRSLLSYWHNHPSLSYLFSGIFVGPTSQAPRVDEARHESLNELEIAFRELD
                     RNTFTPLWLVDRLFRNLLTDLTGNTHRAEFCIDKLYSPDSSSGRLGILEMRAFEMPPH
                     TRMSLAQMLLIRSLVAKFWNHPYQKSLLRHGTILHDKYLLPHFIWEDFKEVIFDLNDT
                     GYEFDANWYKVFLDFRFPIYGKVQLGPITLEIRMAIEPWLVLGEEGIQGGTARYVDSS
                     LERVQVRVFGMTGKRHIITCNGRKIPIHPTGVNGEYVGGVRYRAWQPPSALHPTIPIH
                     SPLIFDVVDTWNNRAVGGCTYHVSHPGGRGYTIFPVNSNEAESRRFSRFYPFGHTPNG
                     LKVNYEELNPEFPYTLDLRK"
     gene            complement(20078..20425)
                     /locus_tag="PLF66_17950"
     CDS             complement(20078..20425)
                     /locus_tag="PLF66_17950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HMZ65979.1"
                     /translation="MNIDWSRIEELISPEDPDDVLWLKGYLNDLIVAYDGYFTELNEG
                     LNKRDQKNVISILHQMKGVAANNGLETVFSLIKDNEQYSKENNLDKVFSQRAILKENW
                     DLTKKEIQKKFNL"
     gene            complement(20691..21218)
                     /locus_tag="PLF66_17955"
     CDS             complement(20691..21218)
                     /locus_tag="PLF66_17955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65980.1"
                     /translation="MNTKFLTIKAIVVACFVLASFGLSAQQAAAQQTKDFSNTVVLKN
                     GTEINNANVEEAYVVTTTDGKSTVYKKDEVASVSKLENRAGSENWSANLGYMNWEDAK
                     AKCASQKMRLPTLDELKAAQSSNVTDSWKKQGYLYWSVTQTPTLLGDPYLLYAFNGEV
                     YSTSRYANNYVRCIK"
BASE COUNT         7297 a         3569 c         3625 g         6933 t
ORIGIN      
        1 tcgtaccctt tgtttccttc ctttaatttt ttatttcatc accctgatag gttaccgttt
       61 ttctaatgga tcaataaata agcgggtttg ggcagccgtt caaaaaagaa ttaaccacga
      121 agagcacaga gaacacgaag aaaagagaag gtttattatt tattctcttc gtgagtcttc
      181 gtgcgcttcg tggttgaaaa tccgtaaatt gacagcattg gccgacaggc tccctttctc
      241 tcatcttacg cgctcatgag taaggtgagt taattaaaaa aggatttgct taattaagta
      301 tttacttaat taatgacata acaattattt gccggagata tttatgaatg ttttttctgc
      361 actcgcggat acaactcgtc gagacatcgt agttcttctt gctcgaaaag gggaactttc
      421 gatgagtgat atatcgaaaa attttactat gacgcctcct gcaatttcac agcatttgaa
      481 aattttaaaa gaggcaaagg tcattcaagt aaaaaaagag gcacaactgc ggctctatag
      541 cattgatcaa tctggactag atgaaatgga tgaatggatc tcagaggttc gcaacctttg
      601 gacaaaaaga ttcaattcac ttggaaatta tttagagaaa ctaaaaaagg aaagaggcat
      661 tggaaaaaaa tctcgttaag acaattcgaa aggacaataa agttagcttt caaatatttt
      721 ttgattctcc gcttgatcta gtctttgaag tctggtctag ttcagaacat ttaggacaat
      781 ggtggggacc ggatggattt acaatcacca caagtcagtt tgatttttca aatgggggaa
      841 tttggaattt tgtaatgcac ggtccggatg gaactgacta ttcgaatcag attgagttta
      901 tagacatttc atttcaaaaa catatttttt ataagcataa aggcacaggg gaaactaatt
      961 caggaatcca ttttgaaacc aaagtttttt ttgaaaagct ggaaaatgga acattggtaa
     1021 aaacagaaat ggaattccca tctaaagaaa cgttagatct agtagcagat aaatttggag
     1081 ccatcgaagg tggacaagag cacctgaaga atcttggtaa atatattgaa tcaattataa
     1141 ggagagatgt atgagtaaat taaaagtttt gtgttttggt gtttctacag atggttatgg
     1201 agccggtcca aatcaaagtt tagaaaatcc aatgggggaa ggtgggatgg ctttgcatga
     1261 atgggttttt catacaaatg aatttcagaa aatgcacgga gaaatggaag gtggaattcc
     1321 aaaagtatct ggttcctttg gaatcgataa tgattttgcg aaaagaggat ttgaaaacat
     1381 aggttcttgg atcttaggtc gatatatgtt tggtccatcg cgcggtcctt ggaaaaaaga
     1441 agacgactgg aaaggttggt ggggacctaa tcctccctat catacatccg tatatatttt
     1501 aacgcatcat ccaaaagatc caatcgttat ggaaggtgga acaacgtttt atttcgtaac
     1561 agaaggaata gaaaaagctt tagaattagc aaagaaagct gccaatggga aagatgttcg
     1621 cttgggcggt ggagcaaata ctgtgagaca atatttaaaa gctggtcttg tagatgaact
     1681 acatcttgcg attgctcccg ctcttctagg ttccggtgaa actcttttaa aaggaatcaa
     1741 tcttcctgaa cttggatatc gtataactga atttgtaaat tcggaaaaag caacccacgt
     1801 tgtgtttaaa aaataaactt aagaattttt taaccacgga tgagcacgga taaagatttg
     1861 aattattaaa cccatctgtg ttcatcggtg gcaatcttta atttctttgt tacatcaaat
     1921 tgattttatt tcccagaaag agtttttgag tgagatactt tatagaaaaa tccttcatat
     1981 tcagcaggag tctgtcctcc attgatcatg acggctatcg gctttccagc gacaagtttt
     2041 tgagtgctaa tatattttag aaaatttcca tcagtatggt agctatttcc aaatcctgct
     2101 aaaacaaaaa tatttttata tttcccttct gcttttattt cgtctacaat tttgagaacg
     2161 gtcttggttt taccttcagc gtatgtaatt ggcaaaatca ccttctctgt caaaattcca
     2221 ttattatctt ctagattcac tccccgaatt tgagttaaag gaacgttaac cgttgttgaa
     2281 gatcctttca caaagaaatc aggacttgca gaaattatat aaactctaaa atcactttgt
     2341 tctcgcatag agttaaatat ttcgatactt tgtgagaaat aataattttg taagactgat
     2401 tcaaaatgat ttgtagaaaa tctttccatc tcctttcgat ctctacctgc aaaaatttgt
     2461 gcgatatagt cataggctac gatatgacct ttcgttttat ccaatctctc ttctgctttt
     2521 acaaatttct cgtatccatt tttgtcatac tttggcgata aacccgcttc aatacttacc
     2581 tgttctagtc ctttataaac tacttgtcca ttttcaatca atccttcact acaatcccct
     2641 ttgaggatcg ttccatcaaa atcccaaaaa cttaaaaaca aacaatcttc tggagtctcg
     2701 cagagttttt ttccattttc cttgtatgcc aagatttctt tttttgcatt ttgaatctgc
     2761 tgaataattt cttttgcctg tgtatcaggt gtttcttgcg aaatagtttt ctttccacag
     2821 gatgcaaata aaaaggatag catcactatt aatataattt gattgattgt tttaatcttc
     2881 attgtttatc tccatgtttt tattgataac tcaccttttg tatggtgaat tattacaaat
     2941 aaactttttc tatttgcgat ggcaatccga aagaaacttt acatagtttc tattcaaata
     3001 aattttagaa caatgactct ctatttactt tttttaatta tccatatttt ttccgcaatg
     3061 ttttggattg gtggaacact cttttatgtt tttatcatct ttaaagtatt aacaaaggaa
     3121 gatatgaaac cttttaagtc tttagtgata aaagagtctg ctcttcaatt tagaaatata
     3181 tcatacgtta ttttcatgac ccttttcttg tccggcttag gtctgatttt taaaaaacac
     3241 aattggagtg atttaatcca tgggctaatt agtgttaaac tagttctttt tatattattg
     3301 ctcatctcat ctatttatca tgactttgtt acaggaccca aaacattttt attctctgaa
     3361 acagatttaa taaaatacaa tcaatacaga aaaatttcat cctactttgg aagaatcaat
     3421 ctattgattt caatttctat tgcaattcta ggagttctta tatcgagagg aattaaatga
     3481 ctcatagaaa gaggtattta acgcttagta tcatttcttt tttgatttgt gtgctgattg
     3541 tattattgtt ttcgaatcat ggctttatcc gtggctttct aggagatcta atcatagtta
     3601 tcttcctttt cttttctatt aaattgattt atcctttttc aaattttgct ctttctctct
     3661 ctgttgtaat ctttgcatat tgtattgaat ttactcaata cattaagatc attccccttt
     3721 taggattaaa agaaaatata tttacaaaaa tagtttttgg atcggtgttt gatccatttg
     3781 atttactcgc gtatacagta ggaggagtaa tcagctaccg aattggaaat aaaatcgaaa
     3841 attagtataa caaaaaataa ctaactcaaa aacctatcgg tgtccatcgt ctttattgga
     3901 ggaagtcagt cttcttgtaa ctgtgggtaa ggttagttcc tattatgtat gaatttttac
     3961 gttgaaaata tttgagaata cattagaata tacaaagccc caatgaaaac aatccctaat
     4021 tcatcccttc acaatttaaa aagaagaatt cttctattcc ttctattttt tctaattaca
     4081 cctatccacg gagagaatat catttttgat ttagcagata aaaaggatta tcctctcatc
     4141 ttaacttccc atgcaacagt attagaagat tcatcccttg atttaaaaat agaagacata
     4201 ctatcaccga aacacaattc cgaattcaaa ttgagaaata tttccggcaa tgcctataat
     4261 ttttcataca cagactctgc gatctggata aaactttcga ttcgcaattc agaaccaatt
     4321 acgaaagatt attttttaga aatacaatat ccgaggctaa tgaatatttt cctctattta
     4381 cctgagggta atagttataa aaaagtgaac tctggatact cggttccaca agatgagcgc
     4441 ccatacaaaa gtcgctattt cacttttccc attcggttag atgcaaataa aactagctcc
     4501 atttatctaa gaatcacgtc tcctaacgcc attgtgctac ctttctttct atgggataca
     4561 acgtcttatc acaatcatga tgtagaagat catattattc aatctctata ctttgggatt
     4621 ttattttcca tgattgcgta taatctactt atttatattt tcttacggga taaatcctat
     4681 ttatactatg ttttatttgt aatcagtagt ggagttggaa ttgccgcttt caatggaatt
     4741 gctggagaat ggctttgggg agataatcct tatattgaga aaattggagt taacgcatgg
     4801 agcgtgattg cttgcacaac gttaatgttt tttatgagaa ggatgttaaa ttcaaaaata
     4861 gataccccaa ttctagataa atttaactgg ctaaccatgt ttatccaatg gacagcctta
     4921 tttttaatgc tttgggattt taaaggattt gttaagttag taattttttc tcaaatattt
     4981 atagcaggct ggattacttt ttatagcatt attcgctcct tccaaaaaaa tagagcttct
     5041 tatttctttg taactgcctt tacctttctc atgattgcaa tttcatccgg gtatctgaga
     5101 gtgctaggga ttttaccaat caataaattc acaacagaag gagtgcaaat tagctctgcc
     5161 attcaaatga ttcttcttgc tatagcgtta gctgatagat acaatacact cagaaaagag
     5221 aaggaaagag cggaagcact tatccaagaa aatttaaaaa aatcaaatct agaattggaa
     5281 gagaaagtaa aagaaagaac acatgaatta aataagtcgc ttcatttaat caaagaagat
     5341 ttgaatttag ccaagaaaat tcaaatgaac gctctttcga tgaatccaac tgatataaaa
     5401 gacttaaagc ttgcaataaa atatctacct atgacagaag tcggtggtga cttctttgac
     5461 ataagtaaga ttgatgatag gacatatcgt atttttattg cagatgctac cggtcatgga
     5521 gtgcaagcag ctatgatcac aatggttata aaaggcatct atgataatat taaatcattc
     5581 ggtttgaaac caaatgaaat tttagaaatt ttcaacaatg aatttataca taaatatata
     5641 tctctaaata gttacctaac ggcaattgta gtagatattg ataccgaaag caaaacttta
     5701 acctatgttt ctgctggtca tccatcctca attctcttat caaggggaga aatcaaagaa
     5761 ttaacacgaa cagggaaaat cattggagtc tttaaaggaa caaactatca atcgatcaca
     5821 ataaattttt cagagaaaga caagcttttc ttatttacgg atggaatttt tgaggaattc
     5881 aataagtccg aggaagaatt tggagaagag aggctacaag agattatccg caaaaaccat
     5941 gataaatcta tttctgaaat tatagatata tccctagatg aactgaattc ctttcttggc
     6001 gcaaaagaaa aacaagatga tattactata attggaatcg aattaaatta atctaaacag
     6061 tttctaaaat atttaaatac ttttttgaag ctgggtttac acaattacaa ataaaaatgg
     6121 cactgcaaag attgaggtag gaatcaattc aaattctcta tagatttaga aagcattttt
     6181 agattcctta aaaatccatc ttctctaaaa aatcaccttt tttatagcgg gtaatggtcc
     6241 gtaaccttca aatcgtgact atttttctaa atctaaaatc tgattcatta tgatttctgg
     6301 tttcaatgag gtatatgatt tcgtgctaaa ttctaagata ataaatcctt tgtctccact
     6361 gccaagaata aattttttaa atagtataag cattggaacc aaatcgagaa ttgcgataca
     6421 gatttgcagt aaggagtgga gtagtaaatt catctctttt tgcttttgta aaaaagagaa
     6481 ttagattttt ctatctaact aacgtgtttt actctacata agttgtaatc aatacagcaa
     6541 acccataatt cattcataga aatcttttta aagaaaaatg aagaaatact tgtcagtaag
     6601 tatttgaaat taggacatag ataaggaagt ttataagtcg ttaaaacaaa tgagagcatt
     6661 aatattagaa aatagtaaat tagttcgaaa tatgattaga gccatttttg aatcagataa
     6721 gtttgaagtt tttgaagcag gaacatcaag tgaggcaaat caaaattatg cacaagaaac
     6781 attcgatgta atcacattaa gtctctattt agaaggggaa gatggttttg atatatgccg
     6841 ccgaattcga aatgcaaaag aaggagaagc tttttacaat tccaaaagtg cagtgattat
     6901 ttttataact tcaaacgaca caattgaatc taggctaaaa ggtttttccg ttggtgctac
     6961 cgaatttgtc ctaaaaaaga atttagaaga aggcttaaaa ccaattatta gcaatctcat
     7021 aaacccaaaa ggaaatcttg aaaatacaaa aatattgatt gttgatgata gctatataaa
     7081 tcgcttgatg atcctgcaag ttttgaaaaa taaaggagca gaactaatcg aagcggataa
     7141 cggcaaagaa gcacttaatc ttatcaaaga gagagacaat aaaatagatc tgattctaac
     7201 tgactattat atgccagaaa tgaacggcac cgatctctgc ttggagattc gaaaaattta
     7261 ttttctaaaa tatttacccg tcattattct aacaggcgcc gatgataagt cggaagtttt
     7321 atctatcttt caagctggag ctacagacta tatcataaag ccattcataa aagaagaatt
     7381 gctcgctcga atcgaaaatc atttagaagc atggctctat cgaagagaga ttttaagtaa
     7441 attacatagg atatagaatg tggataactt tcgttatccg tgataaaaca agatttttga
     7501 gttttcaatc taccattgtt ttatgattct ttttgctaaa ccgattcgta gtaatcttta
     7561 aaatcctatc ccgttcttac tataacaaaa acgggatagt ttaaatcaaa acttataaag
     7621 ctttaattag attcactcca agaatctctg ttgcagtcat gaaaccagaa gcgagagcac
     7681 tagcaactcc tgcaaataat atatcctgtc ctgagatgta gagactatcg ataggcgatt
     7741 taggtctcat ccatttttct gcgaatcgat ccggagtatg atccaatcca tacaattctc
     7801 catgctggta tttgttgaaa tattcagttg aaagaggagt agagagttca taaaaatcga
     7861 ctttcccttt tacttcggga acatatttat atacataatc gagaagcctt tctgaaaact
     7921 tttcttttaa ctcattgtaa tcttctccgc gttttctcca aggtttatcc ttccactttt
     7981 gaaaccattc ataggcagct ggagctatta catctatcgt tgattttcct tcatagtgca
     8041 agtcccattc tggatcttta aaggatggaa atgaaatata aaccaaagga aaatcggaat
     8101 tctgattttg taaatatttg gaataagttt tatcatggtc ataatcggga taaatccata
     8161 gatttccatt ttctagtttc aaatcttttt tagaagcttt gattccagtg tataaacaaa
     8221 tatgacccac agatggcttg atctttttca aatttgataa aagacctaga tcggaagcaa
     8281 tgtcctttgc aattaatttt ctataagtat tgatatatcc agttgcactg ataacgatag
     8341 gcgctaaaat ttcatctcca ttttccattt taacaccata ggctttgttg tttttaatta
     8401 gtatttcgga aacttctttt cctactaaaa tctttccatt tgacttttgg ataacttttt
     8461 ctatcgttct agcaatacta gaagcaccac ctactggaaa attccctccg tctaagtaat
     8521 gatgcgcaac cacagcatgc attgcaaaac tagattgctt tggaggaagt ccataatctc
     8581 cccattgact agacaagaca gaaattaatt tgggatcatc tgttatctca ctcaatacct
     8641 caaaagtagt tcttccgaaa tacttcttta aaattggttg cgttataaga cccgaaatgc
     8701 ctgataaaaa agaaggggtt agcttctgag ttacgatagc cggcattgcg agtaggttta
     8761 cttcatccaa taggctaaga tatttttcca gagatgtttt ggcatttgga aaatgtttta
     8821 aaagctctgt tacaaaattt tctctacctg ctctaaaatc ataccgattt tcttgaataa
     8881 aaatcttatc atagacagga tccatatacg accattttaa ctctccatcg cttatccaat
     8941 catagatttt tctgagagga gaccattctt tatgcacatc tccgatatag tgtaagccta
     9001 catcccattc atatcctttt cgagaataac tgtgagtaaa ccctcccgca gtgtaatgtt
     9061 gttctaaaac aagcacagcc ttaccagcct tagacaaaat agctgctgtg gttagcccac
     9121 ccattcctga gcctattacg attgcatcgt attttttact cgcatcttct gtattgtatt
     9181 tctttccaac attggacata taagtcctcc ttatttcgaa tgtttacact gtaaatttta
     9241 atgtttatac tgtcaacatt tattttattc aaattcatga gatattttat ggatggatac
     9301 tttcgaacat ttgctcttat ctgacctaga atttaatttt tacgattgac aaaacttttc
     9361 taataggtaa tatattacct attagaaaag gagaaaagcc atgaaacgat ttatcttatt
     9421 tatgatagtt ttaaaaatga tcggttgtca ttctgtaaag gatactttta tagaaaatgt
     9481 atcttccgaa tttaaagaac aaccaattca agaaaaagaa attctaactg agaaggactt
     9541 aaatggtctt ccaactcttg ttcagaaata ttacgataca ctggctctat tggtaaggaa
     9601 aaaattcaaa actttagatt ggaattctac gcagaaatga gaaagacaaa tgaaagtgct
     9661 cccatgaaat cagacactat ccagtataat ttctatacca atccagcaag acatttctat
     9721 ttagaaacaa gtatgttttt gattccagca agagttttac actcctactc aaaagaaaaa
     9781 gcaagtatgc aggtaagaat tgcgaattta ttcaatatta caaatataag tggcgaggaa
     9841 ttatcgaaga cagagacttt gaccctgtta aatgatcttt gtttttttgc acctgctgca
     9901 ctgattgata aaagaatcca atgggagaca atagacgata catcagtaaa agcaattttt
     9961 caaaatggaa gacacaaggt tactgctgtt ttgtttttta atgaattagg tgagttgata
    10021 aatttcacaa ccgatgaaag aggagctctt caggatgata atacgatgaa atatgtaaaa
    10081 tggtctactc caattggaaa ctataaagat ttcaatggaa ggaaaatcgc cacatatggt
    10141 gaagctattt accattatcc ggaaggggat ttaacttacg gaaaattctt tttaaggaag
    10201 attgattaca atgtcaaaga attctagact aattaatacg agagatgcat tcggattatt
    10261 atttttagtc tcaaatgcaa tagagataat tgctgataaa catctctctc cctataaaat
    10321 tactacaaaa caattttttc tatccttaat attaattcaa aatcaaaagg cagcactaac
    10381 tatcagcaaa gcagctagtc ttcttggcac aagtagacaa aacacaaaac agttggctct
    10441 caagttagaa aaaattgggt tttgcaaaat tattccaaat gaaaatgata aaagaatctt
    10501 aaatatagaa ataacgtcta agaatattga cttttggaaa agaattgata aagaaaatca
    10561 agccttccta aaaaaaacat ttgaaaatct gagtgatcaa gatatctctt ctttaaaaga
    10621 aggattgatc aaattataca atcaaatcca agaggaaaag taatatgact atttcaagaa
    10681 aagatttttt agataagaca acgcatctag gtttattact aacgacttct caactccttc
    10741 aatactgtag tagtcttcct gatcgatatg cggatacctc tccatttgat caagaaaatc
    10801 tctcacctct ttggaaatct atttacgcag gaataggagc tccgaaccct cataatacac
    10861 aagcttggaa aattaaaatt ctaaacgaaa atgagattct gctttttgta gatgaatcta
    10921 gaattctact cgatacagat cctccagcca gacaaattca tatagggcaa ggttctttta
    10981 ttgaatgttt ttcgattgga gcagcccagc ttggttatac aacaaaagtt caatacttcc
    11041 cacagggtga atataaaaca tccgagattg gaaaaaagcc tgtcgcaaaa ttaacattat
    11101 ccgctggtga tagtaataag aaaaagtttt ttaaaagttt aaatattcga aatacaaatc
    11161 gttctattta cagtggtgac ttttcagaaa ctgatagaga caaaatccaa aatgaaattc
    11221 aatatgataa aagtaatttt aaatggatac tctcctccga ttcccgtttt gcaaaaatac
    11281 aagacttact cgtagaagca tttgcaatcg aaacaaacct aaaaagaacc aatgaagaaa
    11341 atcgaatttg gtttagagtt acagacaagg aaatttactc taagcgagac gggatttcac
    11401 tacgtggaaa tggaatgagt ggattgtcgt ataaaattat cagtaatttc ttcgtttcta
    11461 caaaacctga agattggcat tcagacttca caagaaaagc aggaattgat tcttttaaag
    11521 aacaagtcaa atccagtaag gcttttatct atatactaac gtctaaaaat gaaatgaaag
    11581 actgggttga atcaggtgag aaatacgctc acctaaatat tgcagtcaat tctctttctt
    11641 actcaatgca ccctatgagt caatttctcc aagaatatcc agaaatggaa aaggtaaaat
    11701 tagaattcga aaaattaatg gaaatccctc ccaaagcaaa gattcaaatg tttgctcgaa
    11761 ttgggaaatc agattatttt ttccagagtc caagaagaaa cccacaggat atgatcgtaa
    11821 agagttaaga taaattcatt tacagcaatg ttgaaatttt ggtattgtga atgtctatga
    11881 ctcgaatttt acttttttta ttactgattt cttccgtgtt tggagaggag actccctact
    11941 ctaaagttaa aattactcca aaaaatgctc tcgtcttaag aacaggagga gaaagcgatt
    12001 ttgaggttaa tattaattta cctcataaac attatctata tctatctcat acaaataagg
    12061 atgcaatcgg tattctcaca aatttttctt ttccgattga atccggattt caactaatag
    12121 aaacaaagcg accaaaaggt gttaagaaat tcgatgaaat gattttaaga gaaagtggaa
    12181 cattttcatt taaagcattt gaacttggac tcagaaagaa tggatcaaat gcaaagattc
    12241 ctatgagtat aagaattcaa atttgtgaag agaaagaaaa tccagtctgc tatcctccag
    12301 aaactttgat aaaggaagtt atagcagaag ttagagatga taagagaatt atcaatacaa
    12361 gagaaattga atctatttca tgggaaacag atttcgaaaa tgcaattaca aaggcaaaag
    12421 caagtaacct gaatatctat ggaattatta cagaacctag ttggtgtggc gcctgtcgct
    12481 atatggaaca agaagccttt tcaaaaccgg aaatgaaaaa agttttaaaa gaaaaattca
    12541 taccttggaa aataaaagac gaagaataca ataaagtccc actgggaaat ggaacctttg
    12601 gaatcccaac ctattttgtt ttaaatccaa atggatcttc tcttgcaaaa tggacgggag
    12661 cacgtgatag taaatcactc ttaactctat taaagcctta tgaaaaatca aatcccagcc
    12721 ctgaactaga accatctccc ccttctcccg attcaagtga atttgaagta aaaggaaatg
    12781 atgaaagcaa atgcactctt ctaataggaa aagactacaa tttgatcgca caaaagtctg
    12841 gcgaattttt gaacgacgga aaaattagat ttggtttcaa taatcaagaa ttaaaaataa
    12901 aacaattcag cagaaatgtt tcgaatcgaa aagcctatcc tgttaaattc acgacactag
    12961 gacttagaat agaaaaacca gagataaaag aatactgggt attggaatgc aaaaattcaa
    13021 ttcttgtggg caaaatagaa ggcagtcaaa ttgaattttc aatagagaaa tgagttatta
    13081 cttataccga accaaaatga atttcccatt ttcaatcact cagaagtttt cgatttaaag
    13141 ccttcttagt cttcttgctg tttgtttctg cgatctacca aactctactc caataaaatc
    13201 tttctgaagt ttattttgat taggtatcta tttaataaag ttaacgaatc gaataacaaa
    13261 agttatttgt aagaatatta attatatcat acacccgcgg cgaaaagttt ctgctaaaag
    13321 caaacctcaa tgaatcgatt ggtgaacctt ggtcaaggtg atttaattat ttagaatttc
    13381 catcttcatc tctttgagat aatctaggct acctgactta agtagagatt gaagagttgt
    13441 tttttctgat gagcttatat agtctttctc gcgaagttga tctaaaactt caacgactaa
    13501 ttcatattcc aaaccataaa gttcagagta aatccattga atcgttaggc gctgcccact
    13561 cttctttaaa tgtttaataa aagaagaagt aatatcatct atttgacctc gacgttttac
    13621 taactcgccc attgtctcta gaataaaaaa tatctccata ttttctctga atagatttaa
    13681 catatgatca taaatcttag gagaaatcaa tgggagatta taaatattat cctcaagtgc
    13741 taaatacaaa ttgcgctcat cgtcttcact cagatcagta tatttatcaa attcggacat
    13801 tacctcaggt ggtaaaatta ataaaaattg tgaagcgagt ttatattctt gtttttctct
    13861 aaccaaaaaa acaatcatat tacaaattct ctgatttgaa actaatttcc aataacggct
    13921 gcttttgagt gcaaagtagt tattaacgtc aggtttcttt gctaacattt tccttacttt
    13981 taaataattt tcatagtcga tttgtaaaat tctgaatagt gtatcgttgc ttaacttttc
    14041 gacaagctct tggacttctt ctggctcaca gattccgaaa aaatatcgaa ttaaatctaa
    14101 cttatcagaa ctttcattag caaactcatc tattacagca gcttgtgctt gctgaaatgc
    14161 gctagccgct acagtaaacg tattttctcc tttaataaat gaatttaatt tattaccagc
    14221 aatacagaac tttttaaaat tgcccatcca tgcagaattg atagacaata cagatctaca
    14281 attaccttga caaacttggt aaatagttgt atctaaaaag catttttgta aatctgagtt
    14341 tgattttaac acgtcgttat tctttcacta attcgccaca ctcacgcata tcgggtgcgt
    14401 agggattttg caccatttcc ccttttgcga cccaatactt tgcaaccatt ggacaataaa
    14461 atttattgta ttctgtttga atttgatttg cttttattat ttcggttaat gtttccgaga
    14521 aacttgagag cccattgaaa aaagcttctt gatccttggt atttatattt gttagagtcg
    14581 attccatttt ctcgatttgt ttttttatcg attcattttt ttccgaagca atctttgctt
    14641 gttgaacact ttctaaaagt gcttttgtat ctggaattag attctctttt gtaattaata
    14701 tctcatggac tttttggttt tcagataaaa cttttagaag ggagtttttt atacctgagt
    14761 caatctgacc tttacaagaa aaaacaaaaa gtagaagaag gaaaatatat ctcattgtag
    14821 aaatcctttt tgctaatatt ttattctcaa gcaaaattaa gtcaatttca aatttaatcc
    14881 aaagttttcg taaattaatt tctttatttt tataaaagga acaaaaaaat gaacattcca
    14941 aagataaaga ttgcaaattg ctttgttttt agaagcagaa agaaaggatt aaaatgagaa
    15001 atataagata tttaaaatta aaattaatcg cactaagttt atttactttt ctatcctcca
    15061 caattttagc tgaaactgtg atcctgagaa cccacgatgt aattcacggt aaaatcacgt
    15121 tccaagatgc gaatgtttta cgaatgaatg atgatttagg gaaacctttg gaaattcaga
    15181 agagtgaaat cctaaaagta tcgtatcgag acgtaaaaga tgcaaaagaa atcaaaagaa
    15241 tcattgaaga ggaagaaagt aaactcccgc cagaccaaag aaaaggagca aaatcagcct
    15301 ccaaaaaaag aacattcgat atagtctggc gatctgctct aattccaggc tggggtctat
    15361 ggaaggcaaa caagaaatgg ctcgctgcct tggtatttgt aggcgttgct ggcgtgggcg
    15421 cttattccgt atcaaaagta aatgcgtttg actctgcggg aaaaacatat gagtctaatt
    15481 ccttactgat tggtttatac gtattaagac aaacacaagc aggtgcaagc tcaaatcaaa
    15541 cagtaaactt aggaactgga attatcttgg ggcaggcggc gtttgcaccc tatggacaag
    15601 cacagaccaa tggaaataat gcaatccaag cacttggttt tgtttacgct gtacaattga
    15661 ttcattccat ttttgtagga agagactatg cgaaagagga accagcaaag ggcatcggaa
    15721 ctaaaacagc gtatggggat ataaacttca atgcaactcc taaaatggca ctgactccct
    15781 ctggactcaa tcgagaaacc tactacgaat tgaattattc gttggatttt taaaatctag
    15841 gaattgtgta aaagcaaata tttccaatca ctaaagaagt atgcactcca catctatata
    15901 gcaccataga aagcttttac acattttcat tctaatacta cattgctgaa aatcggcaat
    15961 tcaagaaagc cttacttaaa tcttattgtg aaaggattca aacgatttcc gctgagaatc
    16021 agccatttac aaaataggat atttccttat tttaggcaat ttgatctata tgagaaagtg
    16081 tcttaaaagg aataagaaaa ggatataagg agttcttaca aactgataac aaaaagatca
    16141 aactttgcat cagagttcgc ctgtgatttt ttgtggaatc tatattactg tctcctaatt
    16201 tcgaaattct aattacaaag aatctgcttt agtaaatttt ttactgaaat aatttctaaa
    16261 agtccaaatt ttgcataaac aatatagatg ctatttttat ccagaaatgg aagtgttaaa
    16321 ttagcattca gattattggc aaagcatttt gctcaaatga aagagtatcc tgtgtcgcca
    16381 gattttttac ggatggaatc aatatgtcaa tacgcgtagc tctacatcat aaaactcatt
    16441 atcaatacaa taaaccaatt actatattcc cacaggtcgt ccgattaaga cctgcacctc
    16501 ataccagaac ccctgttgag gcgtattcct tgaaaattca accggggaat cactttatca
    16561 actggatgca ggatccattt ggaaattacg aagcaagact tgtttttcca gaaaaagcaa
    16621 gtagcctaac cgttgagatt gatcttgttg ccaatatgac tgtgatcaat ccgtttgact
    16681 ttttcttaga tgaatccgca gacgagtttc catttgagta ttcagatgat ttaaaagggg
    16741 aactacttcc ttacttacaa attaaagaag atggtgcttt attaaaaaaa ttaatttcta
    16801 aattagattt aaagaaaaga agaacagtag attttttagt ttatatcaat caagtcgttc
    16861 aatctgaagt tggttatatt attcgcatgg aaccgggagt gcaaacaccg gaagaaaccc
    16921 ttactattaa aaaaggttct tgtagagata gcgcatggct acttgtgcaa atgcttagac
    16981 atataggtct tgcggctcgt tttacatctg ggtatttaat tcaattggtt ccagatgtaa
    17041 aatctctaga tggtccatct ggaactaacg ttgattttac agatttacat gcgtggacag
    17101 aagtttattt accaggcgct ggctgggttg gactcgatcc aacttctgga ctctttgctg
    17161 gggaaggtca tatccctctc gcctgcactc caaatcctgc aagcgcggct ccgatcgcag
    17221 gtggaattca aatgggagcg gaagcgaaac ttcagtttga aatgtcagtt actcgaatca
    17281 atgaagaacc tagggtcaca aaaccataca ctgaagagga atggaaaaaa atatatgaat
    17341 taggacataa ggttgacatc gatctcaaaa gaaatgacgt tagactcaca atgggaggag
    17401 aacctacatt tgtttctatc gatgatatgg atggtgccga atggaatgta gaagcattgg
    17461 gtcctacaaa aaaacgatat gcgattgaac tatttaatag attaaaagaa aaatatgcat
    17521 caggacccct tttgcactat ggtcaaggga aatggtatcc aggagaatct ctaccaaggt
    17581 gggcattgtc ttgttattgg agaaaagatg gaatcgctat ttggaatgat ccaaagctga
    17641 ttgcagatga aagactaaca ggaacttata cgaaagaaga tgcagaaaaa tttattagaa
    17701 gattaacaac ccatctcgca attacagatg agcatgtagt tcctggattt gaggacgtat
    17761 actattatct ttggaaagaa ggaactcttc cggttaacgt tgatcctttt ttgaaagatt
    17821 taaaagattc tgaagaaaga aaaactctaa gaagagtatt agaagcagga cttggaacta
    17881 cagttggttt cgttttacca attaaatggg attggcaaag tcgtgtctgg agatctggaa
    17941 gatggcattt gaaaagggaa agactttatt tacttccagg cgactctcca atgggatata
    18001 gacttcctct caattctctt cctcattctg gcaattatta ttatgatgaa gaaccttcta
    18061 gttttgttaa accaaaacct ctcgaaaaca ttcgagaaca agtagcgaaa agataccaag
    18121 cctttgtgaa taaaggaagt agttccggaa gtggaaactc agaattagac tctctttata
    18181 aaaagcattc tgcaaataga gaattggaat taaatgacga tagctgggaa atgatccgaa
    18241 ccgctctttg cgttgaacca agaaatggaa atttatatat atttattcct ccaatgtatc
    18301 atatagagga atatctagat ttaattgcat ctattgagat gactgcagcg gatttaggca
    18361 tgccagtttt aatcgaggga tatctacctc ctcatgatat tcgtttacaa aaatttcaaa
    18421 ttactcctga tcctggggtg atagaagtaa acgttcatcc atcagataat tgggaaactc
    18481 tggttcgaaa cacagaaatt ttatatgatg aagcaagata ttcaagacta ggaacagaaa
    18541 agtttatgtt agatggtaga catacaggga ctggcggagg aaatcacata acgcttggag
    18601 gaaatactcc atcggatagt cctctgctta gaagacctga tctacttcga agtctattat
    18661 cgtattggca taatcatcca agtctatcct atttgttctc gggaattttt gtgggaccca
    18721 caagccaagc tccacgcgta gatgaggcaa gacatgaaag tttaaacgaa ttagagatcg
    18781 catttagaga attagataga aatacattta caccactttg gctagtggat agattattta
    18841 gaaatctttt aacagactta acaggaaaca cacatagggc tgaattctgt attgataagc
    18901 tctattcgcc ggattcaagt tcgggtcgtt taggaatttt agaaatgaga gcttttgaaa
    18961 tgccacctca tacaagaatg agtctagcac aaatgctatt gatcagatct ttggttgcga
    19021 aattttggaa tcacccttat caaaaatcgc ttcttagaca tggaactatt ttacatgata
    19081 aatatctact accgcatttt atttgggaag atttcaaaga ggtaattttt gatttaaacg
    19141 atactggcta tgaatttgat gcaaattggt ataaagtatt tttggatttt agattcccta
    19201 tttatggaaa agtgcaatta ggtccgatta ctctcgaaat tagaatggct atagaaccat
    19261 ggcttgttct aggagaagaa ggaatccaag gtggaacagc acgttatgta gattcttctt
    19321 tggaaagagt tcaagttaga gtgtttggaa tgacaggaaa aagacatatc attacatgta
    19381 atggtagaaa aatccctatt catccaactg gtgtcaatgg tgaatatgtg ggaggagtca
    19441 gatatagagc ttggcaaccg ccatcagcat tgcatcctac tatcccaatc cactcaccac
    19501 tcatttttga tgtcgtagat acttggaata acagagcagt gggtggatgc acttaccatg
    19561 tttcacatcc aggtggaaga ggctatacta tattcccagt aaattcaaac gaagcagaat
    19621 cgagaagatt ttcgagattt tatccgtttg gtcatacacc taacggtctt aaagtgaatt
    19681 acgaagaact aaatcctgaa tttccatata ctcttgatct taggaagtaa aacagaaatt
    19741 tagatttttt gagaaagatt catgttttaa aaacagattc agatgttatt taaaatgtga
    19801 atctcctctt ctctcagaaa ttatctccta tccaaattga ttataaaaaa taaatctata
    19861 aatataaaac cttagagtgt tttgccactc tcttttatta aaaagtcaaa ttttactcaa
    19921 cagaacattt ctaatattat ttaatttttc tatgatattt tcttctttgg actggtatac
    19981 tttcaaaatc tattttggat gagtattgat ttagagaatg aaaaaatgga aggagtgagt
    20041 gaaataaaaa agagttttaa gatgttatgt ttttatatta taaattgaac tttttttgaa
    20101 tttctttttt ggttaaatcc cagttttctt ttagaattgc cctttgacta aaaactttgt
    20161 ccaaattatt ttctttagaa tattgctcat tgtcttttat caaagagaat accgtttcta
    20221 atccattgtt cgcagcaact cccttcatct ggtgcaaaat ggatattaca tttttttgat
    20281 ctcttttgtt taaaccctca ttcaattcag taaaatatcc atcatatgcc acaattaaat
    20341 catttaaata tcccttcagc cacaatacat catcaggatc ctcaggtgaa attaactcct
    20401 caattctact ccaatctata ttcataatcc tctgccttac accaagttaa aaacatttag
    20461 aaaaaaagcg atacaaagat aatccagcct gattggataa tttcaactaa actcaacctt
    20521 tatgttcaaa acagacaaat aaaagttttg attggtataa tcagtttttt ctgagaggac
    20581 aaatcgggaa agtgaatcct taaatttaaa aaggaaatct atttgatgag cagttatagg
    20641 atgttaaatg aaaacttccc agcaacgctg ggaaggcttt ttattaaaat ttactttatg
    20701 cagcgaacat aattatttgc gtatcggcta gtgctataca cttcaccatt aaatgcatat
    20761 aataaataag gatcacctaa aagagtcggt gtctgtgtga cagaccagta taagtatcct
    20821 tgtttcttcc aagaatcagt tacattagaa gattgtgccg cttttaactc atcgagcgtt
    20881 ggtagacgca ttttttggct tgcacactta gcttttgcat cttcccaatt catatacccg
    20941 agattagcac tccaattctc agacccagca cgattctcta acttggaaac agaggctacc
    21001 tcatcctttt tatagactgt tgatttgccg tctgttgtgg ttacaacata agcttcctct
    21061 acattggcat tatttatttc agttccattt tttagaacta cagtattgga aaaatcttta
    21121 gtctgctgtg ctgcagcttg ctgtgcactt aaaccaaagg acgccaaaac gaaacaggct
    21181 acgactattg cttttattgt taaaaattta gtattcatat cttaactatg ttttacggca
    21241 aagttttgta aatgtttttt ctaatttcct tgaacgcgtt ttttaaaata aatcctcaag
    21301 tatagccctt aaatctattt ttagaaaagt aaatagaaaa ttgtatcgag ttctcgccga
    21361 aaatagttga ttagaaaatt cgtaacctat caggctgcta agttactttt atcaagagaa
    21421 tata
//
LOCUS       DAOQOS010000092        21213 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_1210_length_21213_cov_5.724974, whole genome shotgun sequence.
ACCESSION   DAOQOS010000092 DAOQOS010000000
VERSION     DAOQOS010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 21213)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21213)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21213
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1210_length_21213_cov_5.724974"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..640
                     /locus_tag="PLF66_17960"
     CDS             <1..640
                     /locus_tag="PLF66_17960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HMZ65981.1"
                     /translation="DVDKEKMEQIAKFAVEKLERFITNTDFFRNISIEQLNPEEAYIF
                     LKGINSSLSEILKIARVPSNYVMLERTFGLIMGNIAILDPYRSVFDYAEEYFQSIAIG
                     ETGVIEKMLSEEGKELLSTSLLIPKELHKALLTLNRGRLVIHNKDVEKQNEMIYILGN
                     QFIYTLVSIACVFFGNHFLGFYNLIPSLFFYSIASVSGISLLFSIIKNRPKN"
     gene            661..1236
                     /locus_tag="PLF66_17965"
     CDS             661..1236
                     /locus_tag="PLF66_17965"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008594863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein signal peptidase"
                     /protein_id="HMZ65982.1"
                     /translation="MNKFFEKKFLDVIKPHYIIFILLGVMIDLATKYWVIQNYSYHNS
                     ECYMGDFFCMTLTFNTGFVFGIYQNNSIISLFSTAFAIVFLIFYRWKNFEIGNAWGWN
                     LVMVGAFGNFIDKFFIKMPKGGGVKFGFTPIEHGEYIGVVDFLDFDWPDFLLFTRWPA
                     FNFADSCVSVGLVILILTMKADEVKKVEESK"
     gene            1247..1492
                     /locus_tag="PLF66_17970"
     CDS             1247..1492
                     /locus_tag="PLF66_17970"
                     /inference="COORDINATES: protein motif:HMM:NF020917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043231 - intracellular membrane-bounded
                     organelle [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HMZ65983.1"
                     /translation="MSEPKIAQKGPYQVDVKEGEKKAWCLCGHSVKQPFCDGAHSRNN
                     TGMKPVMCQIAQTETVYFCGCKHTKNPPFCDGTHNSL"
     gene            1539..1611
                     /locus_tag="PLF66_17975"
     tRNA            1539..1611
                     /locus_tag="PLF66_17975"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1572..1574,aa:Phe,seq:gaa)
     gene            2359..3567
                     /gene="odhB"
                     /locus_tag="PLF66_17980"
     CDS             2359..3567
                     /gene="odhB"
                     /locus_tag="PLF66_17980"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HMZ65984.1"
                     /translation="MAVEIKVPEMGESITEATLLNWTKKPGEAVKADEILAELETDKV
                     TMEVRSPASGVIKEQKKKAGETVSVREVIGLIEEGAVASTSAPQAQPQPTSEAKATAP
                     IAKQETAKNETLPPSVKKMTTENGIDPSTISGTGKHGQVTKGDVIAHIESKPVASQAP
                     QVKATPRNTGERETVVPMSRLRRAVADRLVTAQHTAAILTTFNEIDMSNVMNLRSQYK
                     DKFKELHNVGLGFMSFFTKAAVAALKAYPAVNAEIRENDIVYKNYYDIGIAVGGPKGL
                     VVPIVRDADQLSFAGIESEIVRLAGRVKDGKIALEEMQGGTFSISNGGTYGSMMSTPI
                     LNYPQVGILGLHNIVKRPVVVNDQIVIRPMMYVALSYDHRIIDGKEAVLFLVKIKEMI
                     EDPTRLLIEV"
     gene            3579..4982
                     /gene="lpdA"
                     /locus_tag="PLF66_17985"
     CDS             3579..4982
                     /gene="lpdA"
                     /locus_tag="PLF66_17985"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004280057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HMZ65985.1"
                     /translation="MSKEFDVAVIGAGPGGYVAAIRAAQLGFKVAIIEKRKTLGGTCL
                     NVGCIPSKALLDSSEEYHKTKHKLADHGINVGDVKLDLKKLLERKDKVVKEVTEGVDY
                     LMKKNKIERFLGHGKLLSTNEIEITSESGTEKISSKKIILATGSSPIEIPGFEIDGKQ
                     VVTSDHAINLPKVPKHLVIIGAGVIGLELGSVWSRLGAKVTVVELQSRLFGTADKQMS
                     NYAQRLLEGQGIQFLFEHKVLGTEKKKDTVIVNIQNPEGKTIQIDGDVVLLAVGRRPF
                     ADNLGAKEIGIQFTNRGRIQVDPHTFQTSIPNIYAIGDVIDGPMLAHKAEDEGIAVAE
                     IIAGQAGHVNYSAIPWIVYTWPEVAWVGLGEEELQAKGIEYKVGKSMFKPNGRAKAMA
                     EAEGQVKVLSDKKTDKLLGLYIIGPRASDMIVEAAVAFEFGASAEDLARTCHAHPTLS
                     EVVREAAMAVDKWSIHS"
     gene            5043..7784
                     /locus_tag="PLF66_17990"
     CDS             5043..7784
                     /locus_tag="PLF66_17990"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008595902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HMZ65986.1"
                     /translation="MALYADNAELLEEMYQTYKTNPMSLDREWQDFFRELDSGISFSN
                     GTNGKNGHKESKTFFKDTDASNLQDMGLMNLLNAYRRQGHLAANLDPLGILKPDRKFI
                     DQKLSNLKPEDLETIVDSGTPGLGKTKLKNVIEWYEKTYCGSIGSEQYYIVNDEEREW
                     IQNKMESTANSYTLEKNAKLRLFKKLYKADFFEQFLAKKFVGKKRFSLEGGEALIPML
                     DLVVEEAGRYKMENLVIGMAHRGRLNVLVNTIQKPASLIFAEFEEKIGSESESYADVK
                     YHLGYSNTIKTESGKEVKLSLAFNPSHLEAVNPVVTGSVRARQTLYNDKTREKFMPIL
                     IHGDAAFVGQGVVAETLNLMNLEGYTTGGTFHIVINNQIGFTTLPSESRSTLYATDLA
                     KGFQIPIFHVNGDDPEAAYRVVKLALDYRQKFRKDVIIDLICYRRLGHNEMDEPAFTQ
                     PYMYNIIKNHPPTAILYEQFLLKEGVVKKEELESIKQEVAQGLEDSFNEAKNKDIKIQ
                     VDTMQGVWSNLSKDPLDSEPATKLLKSQLTNIAKAISTIPSGFTANPKLVKLLETRKK
                     MCEGEIPMDWGCAELLSFGSILESGHKVRLSGQDSQRGTFTHRHAILVDINNNSKYSP
                     LNHISDKQASFEVVNASLSEYSVLGFEYGYSLADPNALVIWEAQFGDFANTAQVIFDQ
                     FISSSEIKWQRMSGLVVLLPHGYEGQGPEHSSGRLERMLQLCAGNNIQIANCTTPAQY
                     FHLLRRQMLRNYRKPLIIMTPKSLLRLPAAVSTVDDLTAGAFKEVLLDKPEIKPEGVK
                     KILFCSGKVYYDLLDARDKQGLNHIALIRVEQIYPFPESGIKEAVNMYKSATEYAWVQ
                     EEPHNQGAWFFVRDFFQHILGNKEFRYYGRKKSPSPAAGHVKVHMKEQEAIVREALS"
     gene            complement(7855..8385)
                     /locus_tag="PLF66_17995"
     CDS             complement(7855..8385)
                     /locus_tag="PLF66_17995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002773347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HMZ65987.1"
                     /translation="MKKILIILTILISTMLVSDCKKEEGTSIGGGMASVDDSTSQKNV
                     VSIAKGSKDHTTLVAAVAAADLVDSLANPGPFTVFAPTNAAFDKLPKGTVEGLLKPDK
                     IEDLKNILEYHVIVGTFKQESFQDGQVIGMANGGNITLSVKGGKVLINGESQIVASVP
                     ASNGIVHVVDSVLLPK"
     gene            8835..9431
                     /locus_tag="PLF66_18000"
     CDS             8835..9431
                     /locus_tag="PLF66_18000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65988.1"
                     /translation="MKKTFPLIPLLLLSVLSIFQYFDLTAMATKKKDKMTELAMSTNG
                     WGGSPDLLKNGTNPNESKSKEYFYLKVNAKASAKSIEKNSELGMKTSCMDAAGLVGTY
                     DILSSFMDALIKEKSPNNETGIVTLELENNKSYTCKYFESEDKKYKTKECSGVLQNRG
                     IAHCWSSPGDSWETCGCLVYLYFPGGKESVLKKLELKE"
     gene            9485..10984
                     /locus_tag="PLF66_18005"
     CDS             9485..10984
                     /locus_tag="PLF66_18005"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002165525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HMZ65989.1"
                     /translation="MSDYVRIFDTTLRDGEQCPGAAMSEDEKLEIAHHLAKMNVDIIE
                     AGFPVSSPVQFKAVERIAREVEGPIITGLARSLRQDIEAAANALKPARKKRIHTFIAS
                     SPIHMKHKLGKSPSEVLQMAIEAVKIARNFVEDVEFSPEDGTRSDWEFLREICEAVIE
                     AGASTINVPDTVGYTTPEKYGELFNFLIKNVKGADKVIFSAHCHNDLGLATANSLSAI
                     VNGVRQVECTINGIGERAGNTAMEEVVMALRTRKDFYGITTNIDTTQIAKASYLVKNI
                     TGMTVQPNKAIVGANAFAHESGIHQDGVIKHRETYEIMTPQSIGLESNRMVLGRHSGR
                     AGFRDRIIRLGFQPKAEELDLAYTRFLEIADKKKEIFDEDIISLFTDQHRKTGVSRFT
                     LDYFHISTGSKTLPTATIKLKVDDVVIEESATGDGPVDAVFKAIEKASKLEPELSRLV
                     ISPVTEGKDALAEASVTLNLNGKRVVGKGSSTDIIEASALSFIDALNRL"
     gene            10981..12069
                     /gene="hisC"
                     /locus_tag="PLF66_18010"
     CDS             10981..12069
                     /gene="hisC"
                     /locus_tag="PLF66_18010"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015680142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HMZ65990.1"
                     /translation="MKNYFQSELYNVAPYVPGEQPKASKKIIKLNTNENPYPPSPKIK
                     KVVQGILSKGLLRKYSNPISSPVVKELAKLHKVPESSIMVTNGSDEGLSILFRATLGS
                     GDIALMPYPTYSLYPVLTNLQLNGAKINQIELRPDFHFDFEKLKKSINPKTKLIAFAN
                     PNAPTGILESKQDLIDLIRSFNGIVLCDEAYIDFAPNNSSMISEINNLDNLIISRTLS
                     KSYGLAGLRVGYLISKPENISELMKLKDSYNLGMLEQEIATAAIQDQKYFKTTIKKII
                     ASRKKLSSDLKKLKFEILESNTNFLFIKPPKTHKAEDLFNYLKTKEVFVRYFKDDYCK
                     DFLRITVGTDSENKNLISHLKIALKEKS"
     gene            12085..12759
                     /locus_tag="PLF66_18015"
     CDS             12085..12759
                     /locus_tag="PLF66_18015"
                     /inference="COORDINATES: protein motif:HMM:NF016277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65991.1"
                     /translation="MIYDEYEFLKVNPESMDVLWMAGFRHFGTKFFRYSIGVLEDDYV
                     QVLPLRIVLDKFTPSKSQKKILKKNKDLTVITRDCFIDESKIELFSIHASRFKDNIPE
                     SIYTFISEKDPANTPCELKEICVYDKERLAAVSFLDIGLESSSSVYAMFHPDYSKRSL
                     GNYTLLLEIEYSRTRNLKYLYPGYAYREPSFYDYKKKFHPMEYFDWNGNWLPFDNTEL
                     GDEFNL"
     gene            12756..13418
                     /locus_tag="PLF66_18020"
     CDS             12756..13418
                     /locus_tag="PLF66_18020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020767913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HMZ65992.1"
                     /translation="MTLTEKLKLQVEQHSVLKSKWFLEKKEKLSRNALNLWLSQEYFV
                     SIDFVNWFLWTATLTSDVQSKILLVHNIWEELGEGDFRKSHIKILVQFLSDIGIEESS
                     LKILPHTKNYLNAMKEITNSNFYSALGALGPANEYLLKLEYGQMYKSYQELRKEENLP
                     NAYFFEVNLEADESHSDQLFRLIETSCDTEKKIEQVINGNQEALDARLIFYEGLQAFE
                     EN"
     gene            complement(13415..13645)
                     /locus_tag="PLF66_18025"
     CDS             complement(13415..13645)
                     /locus_tag="PLF66_18025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65993.1"
                     /translation="MDLHKTIKNAIDLSAPLSELKSILIHYKEEGGTKEDAHMQLYEL
                     LQDASTNNEDFLLRELIDFTIGYSGKIPTIWD"
     gene            complement(13632..13979)
                     /locus_tag="PLF66_18030"
     CDS             complement(13632..13979)
                     /locus_tag="PLF66_18030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016549100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DCC1-like thiol-disulfide oxidoreductase family
                     protein"
                     /protein_id="HMZ65994.1"
                     /translation="MNLFLYDGNCKFCSKLANKLKSICLSKEIEFRSFREIPTKSLKT
                     IHPTLTEEVLFANVQLIQDGSRYPGFFAIRKLSIHLRFYRYFFWVLYLPLIPFIGIMV
                     MNYLKTRKDNGSS"
     gene            complement(13976..15121)
                     /locus_tag="PLF66_18035"
     CDS             complement(13976..15121)
                     /locus_tag="PLF66_18035"
                     /inference="COORDINATES: protein motif:HMM:NF016643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HMZ65995.1"
                     /translation="MKNTSDKLQKFFELTGNETDSLIFLKSFRSIDPEKFLLIYLNPQ
                     VVIESFSTFFYDIKMLHSLGLIPIILLTQDSISYIELFYHFTFQSSGINSEAQPKCDK
                     LLITEKFLENVKNSISKEHIPFLVLESEDNLFGSIANIVKSLKSNKLILLSANGALKQ
                     FSTNKKISMINIRSDYNSILEENLLKDEDLILLNELKDLLENKINHSMNIAITSPVTL
                     LKELFTVGGSGTFIKLGSEIRHLKSIQETDSEKLKILLESAFRKKIESDFLNSKIDSI
                     FLEINFRGAALLKETEYGFLLSKFAVDEIARGEGIGRDIWNEMKKKHKTIFWRAKSEN
                     QINKWYAKECQGMDKGQEWNVYWIQLEIDKIPAVCKFLRSQPKDFVE"
     gene            complement(15118..16245)
                     /locus_tag="PLF66_18040"
     CDS             complement(15118..16245)
                     /locus_tag="PLF66_18040"
                     /inference="COORDINATES: protein motif:HMM:NF013347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HMZ65996.1"
                     /translation="MKTIFNPEALEFHASCAEGLHEFLLTEINSFGLRILSSNRGGVF
                     FRGNKSSLQKFLLSTRFSSRVSFSLNQFTVLDADDLYIQSKRLPWEEILSEGDSFKID
                     SNTKDNLNNSRYALYKFKDAIKDRVREKKGIELDIDRDNPDLVFQLRSNRDFVNIELS
                     LSADPLNKRGYRLEFLEAPIRENMAQALIQFSQWNQSDILIDPMCGSGTILIEAALLI
                     KNKNINEKILNESKAYRMLFDTFISQNQSETESLHLFGYDVNPRAIQIAKENAKRAGV
                     DHLIRFEKSDILQLSNLAKWKSGHIITNPPYGERIGTKDEMKELYGKIAKRLKEEFPG
                     FRFSLVCGDKSLLGYFKLKEDKSMNVSIAKMKGKFVSYNLK"
     gene            complement(16414..16962)
                     /locus_tag="PLF66_18045"
     CDS             complement(16414..16962)
                     /locus_tag="PLF66_18045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010573312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HMZ65997.1"
                     /translation="MKNRKLTLKILFQQEKRFYFFALFVFFMTFWILEDILSLYLNAH
                     WLHYSQTYFTFMEAVIAFFSITGIYFLFKELKDNKTDIEKAEKVIESLQSKNQKLNDL
                     NLDFWKSIQDQYNTWGLTQAEKDIATLLLRGLSNQQIAAIRGKSLKTIENQAFSIYQK
                     SGTTGKLEFIAFFISPLLPEED"
     gene            17099..17467
                     /locus_tag="PLF66_18050"
     CDS             17099..17467
                     /locus_tag="PLF66_18050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ65998.1"
                     /translation="MKKYLIGFLICLCLLFLSNCTSSGKTCDKKCREKGNLCLLGINS
                     SNQASVQNMIVCELYYLDCSSGCGSSSSKSSSSRSRSSSSGGGGSSRSSGGSSSSGGG
                     GGGSSGGSSGSSHDIFVDFH"
     gene            complement(17468..18763)
                     /locus_tag="PLF66_18055"
     CDS             complement(17468..18763)
                     /locus_tag="PLF66_18055"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HMZ65999.1"
                     /translation="MKTRNPRVLVVDDDKYLLEVFKISLEVLDFEIDAISDSEKALSL
                     VSEKDYDLIFLDLKMQPIDGMQILEEVKRQKPNTTVIIISGNSGLDDALKAVDLGAYH
                     ILQKPVQIKELQFFAKKAWEYHAIKSELRELKEAAFSNKRENFITQNATMIKSIETAL
                     SFADSGMNLLIHGESGTGKKLLAELVHQKSPRAKKPFIVVNCNVPENLLEQELFGVRE
                     GAGSASGALTGKLEEALGGTLVLDEIGELSHSIQARLLSVIQSNESKKDIRFISTTTV
                     NIEETLKERLFREDLFYLLNGASIRIIPLRERPEDIEFLFSHFVAGFQKNKPMRMSGD
                     AFHLLKLYRWPGNVLEFKNIIHRAALLTKNDVIEPIHLPDEIQFRNRKDDMDLISLEE
                     VELIHIKKILNRTTDYKVAAKILGIDAATLWRKRKKYKI"
     gene            complement(18825..20726)
                     /locus_tag="PLF66_18060"
     CDS             complement(18825..20726)
                     /locus_tag="PLF66_18060"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HMZ66000.1"
                     /translation="MFKNWVINFSKSKKINLLKNKIFPFMIFLVALYCKPMNLSESLI
                     LNGEHRFMQLQSLSTETPTNCEDFPEKIRVPASTSLYPKFKSYQYYAYCIPFSIDLTQ
                     RKDWGIFLGYIDNNHRIYLNGRLISSTEKKEEMGHTLFYENALIIPFDTANLLPDNVL
                     IVKGRKFNLNSEDGGGIYAGILRIDEYEKLKTANQISKSYGLAKNVLFFSTSLLFFVL
                     FLSRTNNKEYLWFAIFLFAITIYEFCRLELKEDLGIRLESLKYLEYLTLTLLLPIFSL
                     FLYSILYKNKFHIIPMSIVLGGIVFFLLFFFSPNIFYVETLNYTYHVPFLIIVCVILF
                     VATTRELIKKNKRAILVFISCFIPFTLTFFDLLNKKYQFFPILANIQVSGDSILVLVI
                     SMTFYTSYHYYSIQKKLDKTMHKEEMLRKTFQLYVPPRDIEKILSNYDANEELLNLGE
                     LEEKTILFCDIRNFTDLSEKLNPSQTVNFLNSYFKTFNRIIIENGGIIDKLIGDCIMA
                     RFDSGKEYEAIKCSLEMIHALLPYNRARRKMSSGAVNHGIGLASGKVVVGNIGSINKM
                     DYTVIGDTVNIASRLESLTKFYQVSILVTERIQTKLQNQVFFREIDTIQIKGKKKITK
                     IFEPLGLKA"
     gene            20943..>21213
                     /locus_tag="PLF66_18065"
     CDS             20943..>21213
                     /locus_tag="PLF66_18065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66001.1"
                     /translation="MLLILANAILLYLVPDPCEKINTPNKQEIWKLDQVWHVSPLNLQ
                     KKFYCKEDKLEYFYIKQRDRSSLVAIQKVKITKESSCRNNALFSGK"
BASE COUNT         7338 a         3510 c         3549 g         6816 t
ORIGIN      
        1 agatgtagac aaagaaaaaa tggaacaaat tgcaaaattt gcagtcgaaa aattagaaag
       61 gtttattaca aatactgatt tttttagaaa tatttccatt gagcaattaa accctgaaga
      121 agcttatatc tttttaaaag gaattaactc aagcctctcg gaaatactaa aaattgctag
      181 agttccatcc aattatgtga tgcttgaaag aacttttggt cttatcatgg gaaacattgc
      241 tattcttgat ccatatagat ctgtatttga ttatgcggaa gagtattttc aatctatcgc
      301 aataggagaa acaggtgtca ttgagaaaat gctaagtgag gaaggaaaag aattattatc
      361 cacatctctc ttaattccga aagaattgca caaagctctg cttacattga atagagggcg
      421 tcttgtaatt cacaataaag atgtagaaaa acaaaatgag atgatttata ttctaggaaa
      481 ccaatttatc tatacgttag tatctatagc ttgtgttttt tttggtaatc atttcctcgg
      541 attttataat cttattcctt ctttattttt ttattccatt gcatctgtca gcgggatatc
      601 tctactcttc tcaattatta aaaatagacc taaaaattaa attaatacaa ggatttacaa
      661 ttgaataagt tttttgaaaa aaaattttta gatgtaatta aaccacatta catcattttc
      721 attttattgg gagttatgat agatctagct acaaagtatt gggtcattca aaattactca
      781 tatcacaaca gtgaatgtta catgggcgat tttttctgca tgactcttac ctttaatact
      841 ggattcgttt ttggtatcta ccaaaacaat tccattatct ctctcttctc tactgcattt
      901 gcgatcgttt ttttaatctt ctatcgctgg aaaaattttg aaattggaaa cgcttgggga
      961 tggaatctgg ttatggtagg cgcttttggc aattttatcg ataaattctt tattaaaatg
     1021 cctaaaggcg gtggtgtaaa atttggattc acaccaattg aacatggaga atatatagga
     1081 gttgtagatt ttctagattt tgactggcct gactttctat tgtttacacg ctggcctgct
     1141 tttaactttg cagactcttg tgtctctgtt ggtctagtga ttttaatttt aacaatgaaa
     1201 gcggatgaag ttaagaaagt agaagaatca aaataaagga gtatatatgt ctgaacctaa
     1261 aatcgcacag aaaggaccct atcaagttga tgtcaaagaa ggagaaaaaa aggcttggtg
     1321 tctttgtgga cattcagtca agcaaccctt ttgtgatgga gctcattcac gaaacaatac
     1381 aggtatgaaa cctgttatgt gccaaattgc acaaacagag actgtttatt tttgtggatg
     1441 caagcacaca aaaaatcctc ccttctgtga tggaactcac aattcgcttt aaattcattt
     1501 gacgctaacc tatacataaa tattctggca ttagcgacgg ctttgtagct cagttggtag
     1561 agcagaggac tgaaaatcct tgtgtcggga gttcgattct ccccgaagcc agtttgcacc
     1621 taccttttta ctcccaatcc caaatcttct ctcatctcta acttcattta ttacaaacac
     1681 tttctacaaa ttctagaagt cgatagtatc taaagaagac taccctttcc ctttatgatt
     1741 ttatatcaac gtaataagag agatttcaat ctgtttaggt aaatcataca aggtgaatga
     1801 aaagaatgaa aacgaaagtc cctaagaagg ctctgtcaag ttcaggagaa caatttctta
     1861 tttagagaag gtatcgtatt ctttaaaaaa cacgaaaagg atatcaaaag gattgtattt
     1921 gaataaaatt tgctagatac aaaaagctct tttcctttct caagtaggag ttttccttta
     1981 gaacttcgtt tagaaaagaa atacagagta gatttgcatc cgaatcctta caatctcagt
     2041 tgaaaatgaa actagaatcg aatagcaaat tattctgaaa cagttttatc cgtgaaaaca
     2101 taaaatgggg aaataatctt agaccgtatt cgagtaaaaa agagagaaga ataatttctt
     2161 tttcaaagta aaacattcta ggatggaaac aggcatttcc attttacatt catttgattt
     2221 caaagtatcc aatccgatcg atcgttctaa cggaattggt caatttctat caaaaactca
     2281 atttatgaat ttttttattt acgaaaaacc gaaacctaaa ccatatacta ttaaagttat
     2341 aattttttag gatgtaatat ggctgtcgaa atcaaagtgc ctgaaatggg cgaatcaatc
     2401 acagaagcaa ctttactcaa ctggactaag aaaccagggg aagcagtgaa agcagatgaa
     2461 attttagcag aattagaaac agacaaagtc acaatggaag tgagatctcc tgcatcgggt
     2521 gtcataaaag aacaaaagaa aaaagcaggt gagacagttt ctgttcgaga agttattggc
     2581 ttaattgaag agggtgcggt tgcaagcact tcagcaccac aagctcaacc acaaccaacc
     2641 tcagaagcaa aagctactgc gcctatcgca aaacaagaaa ccgctaaaaa tgaaacgtta
     2701 cctccttccg tcaaaaagat gacaacggaa aatggaatcg atccaagcac aatctccgga
     2761 accggaaaac atgggcaagt tacaaaagga gacgttattg cacatattga gtcaaagcct
     2821 gtagcatccc aagctcctca agtaaaagca actcctcgaa atacaggaga aagagaaact
     2881 gttgttccaa tgtctcgtct tcgtcgtgcg gtagccgata gattagtaac tgctcaacac
     2941 acagcagcaa ttcttactac ctttaacgaa atcgatatgt caaatgttat gaatcttaga
     3001 tctcaataca aagacaagtt caaagaactt cataatgttg gcttaggatt tatgagtttc
     3061 tttacaaaag ctgctgtcgc tgcattaaaa gcatatccgg ctgtgaatgc agaaattaga
     3121 gagaatgata tagtttataa aaattattat gatatcggaa ttgcagtggg tggaccaaaa
     3181 ggacttgttg ttccaattgt aagagatgca gatcaactct ccttcgcagg aatcgagtct
     3241 gaaatcgtta gacttgctgg tagagtgaaa gatggaaaga ttgcactaga agaaatgcaa
     3301 ggtggaacct tttccatttc aaatggagga acttatggtt ctatgatgtc tactccaatt
     3361 ctaaactatc ctcaagtggg aatattagga cttcataata tagtcaaaag accagttgta
     3421 gtaaatgacc aaatcgtcat tcgaccaatg atgtatgttg ctctctccta cgaccataga
     3481 atcatcgatg gaaaggaagc tgttctattc ttagtgaaaa tcaaagaaat gatcgaagat
     3541 ccaaccagac ttttaattga ggtttaagaa gaggatatat gagtaaagaa tttgatgtag
     3601 cagtcattgg agcaggtcct ggaggttatg ttgcggctat acgcgcagca caactcggat
     3661 ttaaagtagc gatcattgaa aaaagaaaaa cgttaggagg aacatgttta aatgtgggat
     3721 gtatcccatc caaagccctc ctcgactctt ctgaagaata tcataaaaca aagcataaac
     3781 tggcagatca tggaatcaat gtaggtgatg taaaattaga cctaaagaaa cttctagaaa
     3841 gaaaagataa agtagtgaaa gaagttacag aaggcgtaga ctatctcatg aaaaaaaata
     3901 aaatcgaaag atttttaggt catgggaaat tactttctac taatgaaatt gaaattacat
     3961 cagaatctgg aacagaaaaa atctcctcta aaaaaattat tctagcgact ggatctagtc
     4021 caattgaaat tcctggattt gaaatagacg gtaagcaagt agtcacctca gatcatgcaa
     4081 tcaatttacc aaaagttcct aagcatttag tgattatagg agctggtgta atcggcttag
     4141 aattaggatc tgtttggtct agactcggcg caaaggtaac agtagtagaa ttacaatcta
     4201 gactctttgg aacagcagac aaacaaatgt caaactatgc acaaagactc ttagaaggac
     4261 aaggcatcca atttttattc gagcacaaag ttttaggcac tgaaaagaag aaagacactg
     4321 tgattgtgaa tattcaaaat ccagaaggca aaacaatcca aattgatgga gacgttgttt
     4381 tactagctgt aggtagaaga ccgtttgcag acaatctagg tgctaaagaa attggaattc
     4441 aatttacaaa cagaggtcgt attcaagtag atcctcatac attccaaact tctattccaa
     4501 atatttatgc gataggcgat gtgattgacg gaccaatgct tgctcacaaa gcagaagatg
     4561 aagggattgc agttgctgaa attatagcgg gacaggctgg acatgtaaac tactccgcaa
     4621 ttccttggat tgtttacact tggccagaag ttgcctgggt tggtcttggt gaagaagaat
     4681 tacaagcgaa aggcatagaa tacaaagttg gtaaatctat gtttaaacca aacggtagag
     4741 cgaaagccat ggcagaagca gaaggacaag taaaagtttt atctgataaa aaaacagata
     4801 aacttttagg attatatatc attggacctc gtgcatctga tatgatcgta gaagcggcag
     4861 tagctttcga atttggcgct tccgcggaag atttagcaag aacatgccat gctcatccaa
     4921 ctctctcgga agttgtaaga gaagctgcaa tggcagtgga caaatggtct attcatagct
     4981 aatcatttta aaattgaaaa tttcataatt acaggaggaa tcatatgata aatataaaca
     5041 ctatggcgct ttacgcagac aatgctgaat tgctcgaaga gatgtatcaa acttataaga
     5101 ccaatccaat gagtctagat cgagaatggc aggatttttt tcgcgaacta gattctggta
     5161 tcagtttttc caatggaaca aatggaaaaa atggtcacaa agaatccaaa acttttttca
     5221 aagatacaga tgcatccaac ttacaagaca tgggactcat gaacttgctc aatgcctatc
     5281 gaagacaggg acatcttgca gcaaatttag atcctcttgg aatcttaaaa ccagatagaa
     5341 agtttattga ccaaaaactt tctaatttaa agcctgagga cttagagact atcgttgatt
     5401 ctggaacacc tggactcgga aagacaaaat taaaaaatgt aatagagtgg tatgaaaaaa
     5461 cctactgcgg tagtatcggt tcagaacaat attatatagt aaatgatgaa gaaagagaat
     5521 ggatccaaaa caaaatggag tccactgcaa actcttatac tctcgaaaaa aatgcaaagc
     5581 ttcgcttatt taaaaaatta tacaaagctg atttttttga acaattccta gcaaaaaaat
     5641 tcgtaggtaa aaaaagattt tctctagaag gcggggaagc gttaattcca atgcttgatc
     5701 tagtagtaga agaagcggga cgatacaaga tggaaaattt agtcatcggt atggcgcata
     5761 gaggaagact aaacgtttta gtaaatacaa ttcaaaaacc tgctagttta atcttcgctg
     5821 aatttgaaga gaagattgga tctgaatcag aaagctatgc tgatgttaaa tatcatctcg
     5881 gatactccaa tacaatcaaa acagaatccg gtaaagaggt aaaactatct ttagcattca
     5941 atccgagtca tctagaagca gtaaacccag tcgttacagg aagcgttaga gcaagacaaa
     6001 ctctttacaa tgataaaacc agagaaaagt tcatgccaat cttaatccat ggggatgcag
     6061 catttgttgg acagggggtt gtagcagaaa cactgaactt aatgaatcta gaaggatata
     6121 caacaggtgg aacctttcac attgttataa acaatcaaat tggttttact accctaccaa
     6181 gtgaatcaag atctaccctt tatgcgaccg atttggcaaa gggatttcaa attccaattt
     6241 tccatgtcaa tggagatgat ccagaagcag cttaccgtgt tgtaaaacta gcgttagact
     6301 acagacagaa atttagaaaa gatgtgatca ttgatttaat ttgttatcgt aggcttggtc
     6361 ataatgaaat ggatgaacca gctttcacac aaccttatat gtacaatata atcaagaacc
     6421 accctcctac agccattttg tatgaacaat tcttattaaa agaaggagtt gttaaaaaag
     6481 aggaattaga atctattaaa caagaagtag cacaaggatt agaagattct tttaacgaag
     6541 caaaaaacaa agacatcaaa atccaagttg atacaatgca aggagtttgg tctaatcttt
     6601 ctaaagatcc acttgattca gagccagcta ctaaactatt aaaaagccag cttacaaaca
     6661 ttgcaaaagc aatttcaacc attccttctg gatttactgc aaatcctaaa cttgtgaaac
     6721 ttttagaaac tagaaagaaa atgtgtgaag gtgaaattcc aatggattgg ggttgcgctg
     6781 aactactttc ttttggctca attttagaaa gtggtcataa agttcgtcta tctggacaag
     6841 attcacaaag aggaaccttt acacaccgtc atgcgatact ggtagatatc aacaacaact
     6901 ctaaatactc cccattgaat cacatctcag ataaacaagc tagctttgaa gtggtcaatg
     6961 catctttatc agaatactct gttttaggtt ttgagtatgg ctattctctt gctgatccaa
     7021 atgcgttagt tatttgggaa gcgcaatttg gggattttgc caatacagct caggtaatct
     7081 ttgatcaatt tatttctagc tcagagataa aatggcagag aatgagtgga ttagttgttc
     7141 ttctcccaca tggttatgaa ggacaaggac ctgaacattc aagtggaaga ttagagagaa
     7201 tgttacagct ttgtgctgga aacaatattc aaatcgcaaa ctgcacaaca cctgctcaat
     7261 atttccattt acttagaaga cagatgcttc gtaattatag aaagcctctc atcattatga
     7321 ctccaaagag cttacttaga ttaccagctg cagtatctac agtagatgat ttaactgctg
     7381 gtgcatttaa agaagttctt ttggataagc ctgagattaa accagaagga gtaaaaaaga
     7441 ttctcttctg cagcgggaaa gtttattatg atttactaga tgcaagagac aaacaaggat
     7501 taaaccatat tgctcttatt cgtgtagaac aaatttatcc gttcccagaa tctggaatta
     7561 aagaagctgt gaatatgtat aaatctgcta ctgagtatgc atgggtgcaa gaagaacctc
     7621 ataaccaagg agcttggttc tttgtgagag atttcttcca gcatatctta ggaaataaag
     7681 aatttagata ttacggaaga aagaaatctc caagtccagc tgctggtcat gtgaaggttc
     7741 atatgaaaga gcaagaagct attgtaagag aagctctttc ctaaaaaacg ttagtagact
     7801 tttcttttag agcaaaaacc attttccaag cttttggaaa atggtttttt tattttattt
     7861 tggtaaaagc acgctatcta caacatgcac aataccattt gatgcaggaa ccgaagcaac
     7921 aatctgagat tctccgttaa ttaaaacttt tccacctttg accgatagag taatatttcc
     7981 cccatttgcc attccaatca cctgcccatc ttgaaaactt tcttgtttaa aggtcccaac
     8041 gatcacatgg tattctaaaa tattttttaa atcttcaatt ttgtctggtt ttagcaaacc
     8101 ctctacagtt ccttttggca atttatcaaa tgctgcgttg gttggtgcaa agactgtaaa
     8161 aggtcctgga ttagccaaag aatccacaag atcagctgca gcgacagccg ctacgagtgt
     8221 cgtatggtct ttagatcctt ttgcaatcga aacaacattt ttttgagagg ttgaatcatc
     8281 cacggatgcc atacctcctc caatggaagt cccctcttct tttttacaat cactcactag
     8341 catcgtactg ataagaattg ttaaaatgat taaaattttt ttcatcgaaa ttctcccctt
     8401 tctaatttat aaaacgaatc aatcatatct gatttcaaaa tttgaaatta gacataaaat
     8461 cattaaaacc tatcataaag ttaatttgtt tcaaaaacct tgatctaaat caatttaggt
     8521 aagaattttt aagatgaata atatgacatt tgtaagtaat tcagtgacct tatattttaa
     8581 ttagttttac aacttagatt atcatcgttt gtttttgaaa ttaccaattt tgagcaatgg
     8641 gatttagtaa aaataccagt tttttaaaaa atgttttatt ttacaaaaga ctaaaagcca
     8701 tttaaaatgt ctctaatgcc attagttaaa aactttctct cttctcttct gatataaaac
     8761 tagaaaatgt ctatctaata gtgcttcgat ttttcaattg gcaagaaatt cagtttagaa
     8821 agaatgttgt tactatgaaa aaaacttttc cattgatacc tcttctactc ttatcagttt
     8881 tatctatatt tcaatacttt gatcttactg ctatggctac aaaaaagaaa gataaaatga
     8941 ccgaacttgc catgtctact aacggttggg gaggatctcc ggatctttta aaaaatggaa
     9001 cgaatccaaa tgaaagtaaa tcaaaggaat atttttattt aaaagtaaat gcaaaggcat
     9061 ccgctaaatc gattgagaaa aattcagaat taggtatgaa gacatcttgt atggatgcag
     9121 cggggcttgt gggaacctat gatatactct cttcctttat ggatgcactc atcaaagaga
     9181 aatctccaaa taatgaaact ggaatagtaa ctttagaatt agaaaataac aaatcctata
     9241 cctgtaaata ctttgaatcg gaagataaaa aatataaaac aaaggaatgc tcaggagttt
     9301 tgcaaaatag aggtatagca cactgttggt caagtccagg tgactcatgg gaaacttgtg
     9361 gttgcttagt ttatctttac tttccaggcg gaaaagaatc tgttcttaaa aaactagaat
     9421 taaaagaata aatttttatt tacatatagg ctttttccaa gagtcctact ttaagaggaa
     9481 agaaatgagt gattatgtta gaatttttga tactacttta agagatggag agcagtgccc
     9541 aggtgctgca atgagtgaag atgaaaaatt agaaattgct catcatcttg ctaaaatgaa
     9601 tgtagatata atcgaggcag gcttccctgt atcctcacca gttcaattca aagcagtcga
     9661 aagaattgct cgagaagtag aaggaccaat tattactggt cttgcaagat ctctcagaca
     9721 agacattgaa gcagcagcta acgcattaaa accagcacgt aaaaaacgaa ttcatacatt
     9781 cattgcatct tctccgattc acatgaagca taaattaggt aaatccccat ctgaagtttt
     9841 acaaatggca attgaagctg taaaaattgc acgaaacttt gttgaggatg tagaattttc
     9901 ccctgaggat ggaacaagat ccgattggga gttcctacga gaaatctgtg aagcagttat
     9961 tgaagcggga gctagcacaa ttaacgttcc tgatacagtc ggatatacaa ctcccgaaaa
    10021 atatggagaa ttatttaact tcttaataaa aaacgtaaag ggagcggata aagtcatttt
    10081 ttctgcccat tgccacaacg atttaggttt agccacagcc aattctctct ccgctattgt
    10141 aaatggagtt agacaagtgg aatgcacaat taacgggatt ggtgagcgtg caggaaatac
    10201 agccatggaa gaagttgtaa tggcacttag aacaagaaaa gatttttatg gaatcacaac
    10261 gaatatagat acaactcaaa tcgcaaaggc ttcttacctc gtaaaaaata ttactggtat
    10321 gactgtccag ccaaataagg caatagtagg agctaacgca tttgctcatg agtcaggaat
    10381 tcatcaagat ggagttataa aacaccgcga aacctatgag attatgactc cgcaatcaat
    10441 aggattagaa tccaaccgta tggtattggg tagacactct ggtagagcgg gctttagaga
    10501 tagaatcatt cgtttaggtt ttcagccaaa ggcagaagaa ttagatttag catatactcg
    10561 gtttttagaa atagccgata agaagaaaga aatttttgat gaagatatta tctctctttt
    10621 cactgaccaa catcgtaaga ctggcgttag tcgatttact ttagattatt ttcatatttc
    10681 aactggatca aaaactttgc ctacagcaac catcaaacta aaagtcgatg atgtagtcat
    10741 tgaagaatct gcaacaggag atggaccagt ggatgcagtt tttaaagcaa ttgaaaaggc
    10801 atctaaacta gaaccagagt tatctagact tgttatatca ccagtcacag aaggaaagga
    10861 tgcgttagca gaagcatcgg ttactttaaa cttaaacggg aaaagagtgg tagggaaagg
    10921 gagttctacc gacatcattg aagcatctgc tttgtctttc attgatgcat tgaatagact
    10981 ttgaaaaact attttcaatc ggaattatac aatgtggctc cttatgtgcc aggagaacaa
    11041 ccaaaagctt ccaagaagat tattaaacta aataccaatg agaacccata tcctccgagc
    11101 cctaagatta aaaaagtagt gcagggaatt ctttcaaaag gtcttctacg aaagtattca
    11161 aatccaattt ctagccctgt agttaaggaa cttgcaaaac ttcacaaagt tcctgagtcc
    11221 tctattatgg ttactaacgg ttctgatgaa ggactatcga tattattcag agctacctta
    11281 ggatcaggag atattgcttt aatgccgtat ccaacctact ctctctatcc agtattaaca
    11341 aacttacaat taaatggagc taaaattaat cagatagaat taagaccaga ctttcatttt
    11401 gattttgaga aattaaaaaa atcaatcaat cccaaaacaa aattaattgc atttgcaaat
    11461 ccaaatgcac ctacaggaat tttagaatca aagcaagact taatcgattt aattcgtagc
    11521 tttaacggca ttgttctttg tgatgaagcc tatattgact ttgcaccaaa taactccagc
    11581 atgatttctg aaataaataa tttagataat ctaatcatat ccagaactct ttctaaatcc
    11641 tatggtctag ctggactcag agttggttat ctgattagta agcctgaaaa tatttcagaa
    11701 ctaatgaaat taaaagattc ctataatcta ggtatgctag agcaagaaat tgcaacagct
    11761 gcaattcaag accaaaagta ttttaaaacc acaattaaga aaattattgc ttctagaaaa
    11821 aaactatcct cagatttaaa aaagttaaaa tttgagattt tagaaagcaa cacaaacttc
    11881 ttgtttataa agcctcctaa aactcataaa gctgaggatc tttttaatta cttaaaaacc
    11941 aaagaagtat ttgttcgcta ttttaaagac gattattgta aagatttttt acgaattaca
    12001 gtcggaacag atagtgaaaa taaaaattta atctcccact taaaaattgc tctaaaagaa
    12061 aaatcataat tctctttctt aaaaatgatc tatgatgaat atgaatttct aaaagtaaat
    12121 ccagaatcta tggatgtttt atggatggct gggtttaggc attttggaac taagtttttt
    12181 agatatagta ttggtgtttt agaggatgac tatgtccaag tattaccgct gcgaatagta
    12241 ttagataaat ttactccttc taaaagtcaa aagaagatat taaaaaagaa taaagaccta
    12301 acagtcatta cccgcgactg ttttattgat gaatctaaga tagaattgtt ttctatacat
    12361 gcttcccgtt ttaaagataa tattccagaa tcaatttata ctttcatatc agagaaagat
    12421 ccagcaaata ctccttgtga actcaaagaa atttgtgtct atgataaaga aagattagcc
    12481 gcagttagtt ttttagatat tggattagaa tcttcctcga gtgtttatgc gatgtttcat
    12541 cccgattatt ctaaaagaag tctcggaaat tataccttac tgcttgaaat tgaatattct
    12601 aggactcgaa atttaaaata tctctaccct gggtatgcgt atcgagaacc ttctttttat
    12661 gattataaaa aaaaatttca tccaatggaa tattttgact ggaatggaaa ttggcttcca
    12721 tttgataata cagaactagg agatgaattt aatttatgac tcttacagaa aaacttaagt
    12781 tgcaagtaga acaacattct gttttaaaaa gtaaatggtt tttagaaaaa aaagaaaaac
    12841 tatccagaaa tgctttaaac ctttggctca gtcaagaata cttcgtctct attgattttg
    12901 taaattggtt tttatggaca gcaaccttga catctgatgt tcaatccaaa atattactag
    12961 tccataatat ttgggaggaa ttgggggaag gagattttag aaaatctcat attaaaattt
    13021 tagttcaatt tctatccgac attggcatag aggagtcgag cttaaaaatt ttaccacaca
    13081 caaaaaatta cctcaatgca atgaaagaaa ttacaaattc taatttctac tctgcattag
    13141 gggctttagg accggcaaat gaatatttac taaaactaga atatggacag atgtataaat
    13201 cttaccaaga attaagaaaa gaagagaatc tacctaatgc ctattttttt gaagtaaatc
    13261 ttgaggcaga tgagtcacat tcggatcaac tctttcgttt gattgaaacg agttgtgata
    13321 cagaaaaaaa gattgaacaa gtaatcaatg gaaaccaaga agcgttagat gctagactta
    13381 ttttctatga aggcttacaa gcatttgaag agaattaatc ccatatcgtt gggatctttc
    13441 ctgaatatcc gatggtgaaa tctattaatt ctctgagtaa aaaatcttcg ttattggttg
    13501 aagcatcctg taggagttca tacaattgca tatgtgcatc ctctttcgtc cctccctctt
    13561 ctttatagtg tatcagaatc gatttgagtt cagataaagg tgcacttagg tctatcgcat
    13621 tttttattgt tttatgaaga tccattgtct ttccttgtct ttaagtaatt cattaccatt
    13681 atacctataa atggaatcag tggtagatag agaacccaaa aaaaatatcg gtaaaatcgt
    13741 agatgaatag ataattttcg aatagcaaaa aatcccgggt atcttgatcc atcttgaatc
    13801 aattggacat ttgcaaataa tacctcttcg gttagggttg gatgaattgt ttttaaagat
    13861 tttgtaggta tttctctgaa ggagcgaaat tcaatttctt tagataaaca gattgatttt
    13921 aatttatttg ctaatttact acaaaattta caatttccat catataaaaa caagttcatt
    13981 ccacaaaatc ctttggttgt gatcttaaaa atttacatac agctggtatt ttatctatct
    14041 ctaattgaat ccaatacacg ttccactcct gccccttatc catgccttgg cattctttgg
    14101 cataccattt attgatttga ttttcagatt ttgctctcca aaagatagtt ttatgttttt
    14161 ttttcatttc attccaaata tctctaccga tgccttctcc tcgagctatt tcgtctaccg
    14221 caaattttga aagtaaaaat ccatattcag tttcttttaa tagagcagca cctctaaagt
    14281 ttatttctag aaaaatagaa tctattttgg aatttaaaaa atcagattct attttttttc
    14341 gaaacgcaga ttctaatagg atttttaatt tttcactatc tgtttcctgg attgatttta
    14401 aatggcgtat ttcacttcct aatttaataa aagttccact accaccaaca gtaaataact
    14461 ctttcaataa ggtgacagga gaagtaattg cgatattcat tgaatgattg attttatttt
    14521 ctaatagatc ttttaattca tttaataaaa tcaaatcctc atcttttaat agattttcct
    14581 ctaaaattga attataatca ctacgtatat tgatcattga aattttttta tttgtagaaa
    14641 actgctttag agccccattg gcagatagca agattagttt attcgatttc aaggatttca
    14701 caatattcgc aatcgagcca aacaaattat cttcggattc taaaactagg aatggaatat
    14761 gctcttttga aattgagttt tttacattct ctaaaaattt ctctgtaatc aataatttat
    14821 cgcattttgg ttgagcttct gaattgattc cacttgattg aaaggtaaaa tgataaaata
    14881 attcaatgta agaaattgaa tcctgtgtta aaaggataat cggtattaac cccaaggaat
    14941 gaagcatctt tatatcataa aaaaatgttg aaaatgattc aattacaacc tgtggattta
    15001 aataaataag tagaaatttt tcaggatcaa tagaacgaaa actttttaag aaaattaaac
    15061 tatcagtttc gtttccagtt aattcaaaaa acttttgtaa tttatctgaa gtatttttca
    15121 ttttaaatta taagaaacaa atttaccttt cattttagct attgatacat tcatactttt
    15181 atcttctttt aatttaaaat atccgagtag ggatttatct ccacagacta gagaaaatct
    15241 aaatccagga aattcttctt tcaatctttt tgctattttt ccatataatt ctttcatctc
    15301 atccttggtt ccaattctct caccataagg gggatttgtg attatatgac cagatttcca
    15361 ttttgccaaa ttggataatt gtaaaatatc agatttttca aatcgaatta aatgatcaac
    15421 tccagctctt tttgcattct cttttgcaat ttgaattgct cttggattta catcgtaacc
    15481 aaataaatga agtgattctg tttctgattg gttctgagaa atgaaagtat caaatagcat
    15541 tctatatgct tttgattcat ttaatatttt ttcattgata tttttatttt ttatcaataa
    15601 ggctgcttcg attagaattg ttcctgaacc acacatagga tcaattaaaa tatcagattg
    15661 attccattgc gagaattgaa ttaaggcttg cgccatattt tctctgatgg gagcttctaa
    15721 gaactctaga cgatagccgc gtttatttaa cggatctgct gaaagggaaa gttcaatgtt
    15781 tacaaaatct cgattcgatc tcaattgaaa tactaaatct ggattatccc gatcaatatc
    15841 tagctcgatc cctttttttt ctctaactct atcttttata gcatctttaa atttatagag
    15901 ggcatatcta gaattattca gattgtcttt tgtgttggaa tcaattttaa atgaatcccc
    15961 ttcagaaaga atttcttccc aaggaagtct tttagactgg atatataaat catccgcatc
    16021 taaaacagta aattgattta aactaaaaga gacacgagaa gaaaaccgag tagatagtaa
    16081 aaacttttgc aaggaggact tatttcctct aaaaaaaacg cctcctctgt tactagataa
    16141 gattcgtaaa ccaaaggaat taatctctgt tagtaaaaat tcgtgcaagc cttctgcaca
    16201 cgaggcatga aattctaatg cttctggatt aaatattgtt ttcataattt attctttcta
    16261 ttaactcacc taacgctttc attctatttt aaactaggag tgtgaaatct ttctttatca
    16321 aaaaggattt aacagtcatt tctaattttt ttaaaaatca cttttattta ccgcctctca
    16381 atccctatca tttcttttct aatttcctaa gaatcaatcc tcttctggaa gcaatggaga
    16441 aataaaaaat gcaataaatt ctaatttgcc agtggttcct gatttttgat aaattgaaaa
    16501 agcttggttt tcaattgtct ttaaactttt tccacgaatc gccgcgattt gttgattgga
    16561 caagcctcgt aataataatg tcgcgatatc tttttctgct tgggttaatc cccatgtatt
    16621 gtattgatct tggatcgatt tccaaaaatc taaattaagg tcatttagtt tttgattttt
    16681 agattgtaga ctctcaatta ctttctctgc tttctctata tccgttttat tatcttttaa
    16741 ttctttaaat aaaaaataaa ttcctgttat ggaaaaaaaa gcaatgactg cctccataaa
    16801 tgtaaaatag gtttgagaat aatgaagcca atgtgcattt aaatacaaag ataaaatatc
    16861 ttctaaaatc caaaatgtca taaagaaaac gaataacgca aaaaaataga atcgtttctc
    16921 ttgttgaaat aaaatcttta aagttaattt tctgtttttc atactctatc atgcgaattg
    16981 ctatttctga agtgaatatc tgattcctcc aaagaatagg taatttcatt caatacatat
    17041 attacctata tacagattcc aatttatttg cgagaattaa tttgctggag agaagaatat
    17101 gaaaaagtat ttaatcggat ttttaatttg cctttgttta ttatttttat ctaattgcac
    17161 aagctcagga aaaacctgcg ataaaaaatg ccgagaaaaa ggcaatctat gcctactagg
    17221 aatcaattca tccaatcaag catctgttca aaacatgatt gtatgtgagt tgtattatct
    17281 agactgttcc tctggatgtg gttctagttc tagtaaaagt tctagtagta ggagtcgaag
    17341 tagtagcagc ggtggtggag gtagcagtag aagcagtgga ggcagcagtt cctcgggtgg
    17401 tggtggcgga ggatccagtg ggggaagcag tggatccagt cacgacatct ttgtagattt
    17461 tcactaacta tattttgtat ttcttccttt tgcgccaaag ggtagctgca tcgattccta
    17521 aaatttttgc ggctacttta taatcagttg ttcgatttaa aatcttttta atgtgaatta
    17581 actctacttc ctctagggaa atcaaatcca tatcatcttt tctatttcta aattgtatct
    17641 catccggtaa atggataggc tcaattacat catttttagt aagtaatgcc gctctatgaa
    17701 ttatattttt aaactctaaa acatttccgg gccatctata gagctttaat aaatgaaatg
    17761 catctcctga cattctcatt ggtttgtttt tttgaaagcc agctacaaaa tggctgaata
    17821 aaaactcaat gtcttctggt ctttctctga gtggaatgat acgaatgctt gctccattta
    17881 ataaataaaa caagtcttct ctaaatagtc tttcttttaa agtttcttca atgtttactg
    17941 ttgtagtgga tataaaacga atatcctttt tagattcatt tgattgaatc acagagagaa
    18001 gtctagcttg tatagaatga gaaagctcac caatttcatc tagaaccaaa gttccaccaa
    18061 gcgcttcctc taattttcca gtcaaagctc cggaagcaga acctgcccct tctctcactc
    18121 caaataattc ttgctctaaa agattttcag gaacattgca attcactaca ataaatggtt
    18181 ttttagctct agggcttttt tggtgaacaa gttctgctaa taattttttc ccagttccac
    18241 tttctccatg aattaaaaga ttcataccgc tatccgcaaa acttaaagca gtttctatgc
    18301 ttttgatcat tgtggcattt tgtgttataa aattttctct tttatttgaa aaagcagctt
    18361 cttttaattc tcttaactcg gatttaatcg catgatattc ccaggctttc ttcgcaaaaa
    18421 attgtagctc ttttatttgc actggctttt gtaagatgtg atatgctcct aaatcaactg
    18481 cttttagtgc gtcatctaaa ccactatttc ctgatataat tataactgta gtattcggtt
    18541 tttgtctttt tacttcttct agaatttgca tgccgtctat cggttgcatt tttaagtcaa
    18601 gaaaaattaa atcataatct ttttcagaga ccaaagataa ggctttttcc gaatctgata
    18661 ttgcatcaat ttcaaaatct aaaacttcta gcgaaatttt aaaaacttct aataggtatt
    18721 tatcatcatc aacaacaaga actctaggat ttcgggtctt catattcgaa ttactactct
    18781 ttctataaaa cgttgaatcg aaattatttt ataaccaatt tttatcaagc tttcaaacca
    18841 agtggttcga aaatttttgt gatttttttc tttcccttaa tttgaatcgt atcaatctct
    18901 ctaaaaaaaa cctgattctg taatttagtt tgaattctct ctgttaccaa tatagaaact
    18961 tgataaaact ttgttaagct ctctagtctt gatgcaatat tcacagtgtc gccaattaca
    19021 gtgtaatcca ttttattgat agatccaata tttcccacaa ctactttacc agaagctagt
    19081 cctattccat gattgacagc cccactcgac attttcctac gtgctctatt gtaaggcaga
    19141 agagcatgga tcatttctaa agagcattta attgcttcat attcttttcc agaatcaaaa
    19201 cgagccatga tacaatctcc aattagttta tctatgattc caccattctc aatgatgatt
    19261 cgattaaaag ttttaaaata ggaatttaag aaattaacag tctgactagg atttaatttt
    19321 tctgataaat cagtgaaatt tctaatatca caaaataaaa tggttttctc ttctagctct
    19381 cctaaattga gtaattcttc atttgcatca taattactca gtatcttttc tatatctcgt
    19441 ggtggcacat agagttgaaa tgtttttcga agcatctctt ctttgtgcat agtcttatct
    19501 aattttttct gaatagaata ataatgataa gatgtgtaga aagtcattga tataactaaa
    19561 acaagtatgg aatctccact cacctgaata ttggcaagta ttggaaagaa ttgatatttt
    19621 ttatttaata aatcaaaaaa cgttaatgtg aatggaataa aacaagaaat aaaaactaaa
    19681 attgctcttt tattcttttt aattaattcg cgagtcgttg caacaaatag tataacgcat
    19741 acaattatga gaaaaggaac atgataggta taatttaaag tctctacata aaatatattc
    19801 ggagaaaaga aaaacaataa aaagaaaact attccaccta aaacaatcga cattggaatg
    19861 atatgaaatt tatttttata gaggatagaa tacaagaaca aggaaaagat tggtaataac
    19921 aaggtgagtg ttaaatactc caaatacttc agtgattcaa gtcggattcc taagtcttct
    19981 tttaactcta gtctgcaaaa ttcgtagata gtaattgcaa ataaaaatat tgcaaaccat
    20041 aaatattctt tattattagt tctagaaaga aacaaaacaa aaaacaataa tgaggtagaa
    20101 aaaaataaaa cattttttgc aagcccatac gatttagaga tttgatttgc tgtttttagc
    20161 ttttcatatt catctattct aagaattcca gcatagattc ccccaccatc ttccgagttt
    20221 agattaaatt ttctcccttt tacaattaga acgttatccg gtaaaagatt cgcagtatca
    20281 aaaggaataa tgagagcgtt ctcataaaat agagtatgac ccatttcttc ttttttttct
    20341 gtggatgaga tcaatctacc atttaaataa atcctatggt tgttatcaat ataacctaaa
    20401 aatattcccc aatcttttct ttgggttaga tcaatcgaaa aaggaataca atatgcatag
    20461 tattgatagc ttttaaattt tggataaaga gaggttgagg cgggcactct tattttttca
    20521 ggaaagtctt cacaatttgt aggtgtttca gttgataggg attgcaactg cataaatcga
    20581 tgttccccat ttaaaatcaa agactctgat aaattcatag gcttacaata aagagcgacc
    20641 aaaaaaatca taaacggaaa tattttattt tttaacagat tgattttctt tgatttacta
    20701 aaatttatca cccaattttt aaacatcctt tccccttttc taaaaacata cctgaattga
    20761 tgattttgaa tccactgatt gaacgagaaa atacaatcca aattgaatcc gagtcatggt
    20821 tagaatcata agcattcaaa atcgttttcc catttctttc tttttaggag tcacaaacct
    20881 tatttacata gaatacattg aattgttttc atgtaaaaca ataaagattt ttaggagtca
    20941 ttatgttatt aattcttgca aatgcaattt tactttatct agttccagat ccttgtgaaa
    21001 aaataaatac tccaaataaa caagaaattt ggaaactaga ccaagtgtgg catgtttctc
    21061 ctctaaacct ccaaaaaaaa ttttattgta aagaggataa actggaatac ttttatatca
    21121 agcaaagaga tagatctagt ttagtagcaa tccaaaaagt taaaattaca aaagaaagca
    21181 gttgcagaaa caatgcttta ttttccggta aaa
//
LOCUS       DAOQOS010000093        21189 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_1214_length_21189_cov_5.904987, whole genome shotgun sequence.
ACCESSION   DAOQOS010000093 DAOQOS010000000
VERSION     DAOQOS010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 21189)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21189)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21189
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1214_length_21189_cov_5.904987"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..736
                     /locus_tag="PLF66_18070"
     CDS             <1..736
                     /locus_tag="PLF66_18070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016761210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HMZ66002.1"
                     /translation="RIATIEISRPEALNALNRDLLLELESSIAHLEEDKTTRVAIITG
                     AGKAFVAGADIAAMKEYDYEKAYEFGGTGQVVFTHIERSPIVSIAAINGFALGGGLEL
                     AIACDIRIASKNAKLGLPEVGLGLIPGFGGTQRLPRLIGTGLAKEIIFTADMIPAEEA
                     YRIGLVNKLVEPTDLLTTANTMANSICSRGSHAIREAKRVIWEGLETTQDEGMKLEQK
                     AFGNIFNDKETHEGLSAFLEKRKPNF"
     gene            complement(957..2504)
                     /locus_tag="PLF66_18075"
     CDS             complement(957..2504)
                     /locus_tag="PLF66_18075"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HMZ66003.1"
                     /translation="MLKLDIEKIKQTVTKEELNEAMLPVYRKAEKIMQAMILFHLLLS
                     FILVFYSDTWLVTIGVAVPAVLAYYLVVKLYPDTRFARSNAGIVIQTFMMLHIYQMHG
                     LAEMHFFFFTSTAIMIIYMDWLSIVPMAIYVSAQHLTFILLHNAGWQMYFFEDSYISF
                     TKAFFHYAVAIFQVVISCFWAYTFRQRVLENFYQNRALAKYSEEQLEGKDKVIRNMIQ
                     DLSQVTSSVRESYNDIVNSTNEVSNSLEYIAEGASKQTELADNTVDSSLKLGDRINAT
                     LQTANILIEKSKEMKKENLLGLKQIQNLKDTFNRNQEATKHVNDAINDLSARSRDVAS
                     IIEKIKSIADQTHLLSLNASIEAARAGDSGKGFAVVASEVGKLADESSRSTKEISTIV
                     TEINRFIHSAKQQMLEAESLLKSSEADMNQTVQTFNEIEKTVSEVARNNETLLSEIES
                     MEELKVNSIENSRKILTVIQDSASSTEEISATTKEQSNSVQSILSKINELEQKVKIIA
                     ETLENKD"
     gene            complement(2508..3155)
                     /locus_tag="PLF66_18080"
     CDS             complement(2508..3155)
                     /locus_tag="PLF66_18080"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HMZ66004.1"
                     /translation="MIKRIFVILSLLFLASISAYVIYVEDTRYSLPTPIPTNYENIPI
                     GSTIVPPNPILSVRDKPLLIHFFNPHCPCSRFNLEHFKALVLNYSDKVDFAIVLQIEK
                     ENALEKFKRMDLNLPYVEDKTGEIADRYGVYSTPQAVILDKKGNLYYRGNYNKSRYCS
                     LKSSEYARLALQSLLREEKLEDLPVYAKIAYGCQLPSEGLNQIRIEFLDDLFSIF"
     gene            complement(3152..4939)
                     /locus_tag="PLF66_18085"
     CDS             complement(3152..4939)
                     /locus_tag="PLF66_18085"
                     /inference="COORDINATES: protein motif:HMM:NF012526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016702 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of two atoms of oxygen [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoxygenase family protein"
                     /protein_id="HMZ66005.1"
                     /translation="MWITKICLPQNEKDLKCRNKDLEKKRSEYRYNYNIIPPLAIVDG
                     LPKSEYPSVIWVFKLLQVGVALVKNAIAAREKTAWNFKKFFIPEIFRIKMLFRLLGLL
                     FSGDPTNFFVAGGTYIVKFLKPGEGIDLGEYNDLFGTIPIPKQDIDFQSDENFAIMRV
                     AGWNPTVIEGLDKIPKNFPFTDSLFKSLPLFKNDSIQSAIREKRLFITNYKALEHLEN
                     GYQPNGEKFCYAPMALFAIDKTEKKLRPIAIQCGQDPKKYPIFTPKSSEWAWKMAKAV
                     INSADGNHHELVTHLARTHLFLEPIVVATYRQLSNRHPLYRLIVPHFQGTIFINYLAK
                     AKLIAPGGGVDYLLSGTIASDAGVAVESVLDLKFNDSFFPVSLRKRNVENSAVLEYYP
                     YRDDGLMVWGAIEKWVRSYLEKFYLSPIDIKKDYELQLWAGEISSLSGGRVKGFGEDG
                     NGKLETLDYLVLATTQIIFTASAGHSSVNFPQKDLMIYAPQVPLALYEEAPKANQSYT
                     EKDWIKMLPTLDIASLQLNLGYTLGAIYFTKLGEYPFYWFKDSLKEKPLKNFQKELAE
                     IEKEILSKNKARHPYTFLIPSRLTQSINV"
     gene            complement(4958..6325)
                     /locus_tag="PLF66_18090"
     CDS             complement(4958..6325)
                     /locus_tag="PLF66_18090"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HMZ66006.1"
                     /translation="MTKFEIFPGPTPSFPLGNLNLLLGQNPWEVTSGLAQNYGKIYVL
                     WFLSNPQLVINSPDFFREVLDSNWENFHKDSPLKQLNPVITKYTPFLANQPQWQNMRA
                     KNPFSSDWFQDWLTKQPEILSSGMSTTILALAAKSRSKSLDLTETFQKVSFDSFSLAI
                     AGKYFPDCIYKLFVSLGKTGSKRLVNPFILSFVLNPFFYLKRKKWLGYFEKLISTSEN
                     ELNPNALDLIQWTSGKGANLSDVALTANTADIFYGGVYSVTSNFATVVWLLSQPENKK
                     YEEKLKQEIKSICKNKEIRFADLDKMEFLEKVIRETMRLLPPVPFYSRNSSTTQVLFL
                     KDKQKDREYKIPTNTPIILSNYFLHRTEEHWEDALAFKPERWTSDVIKCNPYGSDYFF
                     PFGRGPRSCMGMPFALMYLRTAIASFYARARFEPEKDAKYKQSFFFGVMMPSGLKGKI
                     VLDQD"
     gene            6689..6889
                     /locus_tag="PLF66_18095"
     CDS             6689..6889
                     /locus_tag="PLF66_18095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66007.1"
                     /translation="MSFIILENTNDLTKSIACDSSAVGKLMKRWEDNPPLLLSRNAQT
                     YCHFLMTIGANLVSLLGSLCLR"
     gene            6993..7697
                     /locus_tag="PLF66_18100"
     CDS             6993..7697
                     /locus_tag="PLF66_18100"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HMZ66008.1"
                     /translation="MKHLILMIFALATCGKKEEQNLQTKGSILFAVGNVTVVSNGNKN
                     SAKKGTPISEGDTILTDSKSTVIVEFSDNAGTIEIQENSEFTVKEFNEKNQELVSKSG
                     SFWLNAKKLKKDTNFNVIMPTSVAGIRGTKLYSFENKELGITGVCHCQGAVEFKENSS
                     NYTGSHDSDFLVLTKAGKTIVITPEDFQKMGYQGSDDNHQHSSLENSPLGKKANLDSE
                     AHRKVLEIADKKWKEK"
     gene            complement(7776..9953)
                     /locus_tag="PLF66_18105"
     CDS             complement(7776..9953)
                     /locus_tag="PLF66_18105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002775359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HMZ66009.1"
                     /translation="MNIRTARQEAISRITDYIPEDSGYDFADTPVPELFGSKVFSIKV
                     MQDRLPKNVFKSLKKTIFEGKPLDPQIADIVANAMKDWALENGATHYTHWFHPLTGLT
                     AEKHDSFISPQEVGEVIMEFSGKELIKGEPDASSFPSGGIRATFEARGYTAWDATSPA
                     FIRETTLVIPTAFCSYTGEALDKKTPLLRSMDALSEQALRVLRLFGNKTATRVTTTVG
                     PEQEYFCIDRNFYNMRQDLILTGRTLFGAKPAKGQESEDHYFGSIKPRIFEFMKELEA
                     ELWLLGVNAKTRHNEVAPAQYEMAPIFSTTNIAMDHNQIVMDTLRKVAGKHGLACLLH
                     EKPFKGVNGSGKHNNWSMSSNDGQNLLEPGDNPHDNAQFLFFLVATIKAVDKHGDLLR
                     VAISNAGNDHRLGANEAPPAIISIFLGSQLTDIIEQIEKGPATTSSKGGFMEIGVSTL
                     PSLPKDTTDRNRTSPFAFTGNKFEFRAVPSSLSLGYPNAFLNTFVAEALDEMATELEN
                     EIKAGSTLEQGVQKVLTANIKKHKRIIFNGDNYTDEWKNEAAKRGLPNFKTLVDALPS
                     MIKPENIAVFEKYKVLSAREVESRYAIILEGYNKVINQEGQCTLSMGRTQILPATLKY
                     QGDLARAIADTKSVLGDGAVKGQIETLKTIASLNETLKAKLDDLEKALDHEDADVLKH
                     AQHFRDSVIPKMNLVREAADKLELIVDSTYWPIPTYGEMLFIV"
     gene            10616..10894
                     /locus_tag="PLF66_18110"
     CDS             10616..10894
                     /locus_tag="PLF66_18110"
                     /inference="COORDINATES: protein motif:HMM:NF014643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HMZ66010.1"
                     /translation="MVWKIANAKSQFSELIHQACVEPQFIYNREKPSAVVISFEEYNR
                     LRNLDILYHKPPKWANFVSYSEKLIQETDLSDGLELPTRDDREIDVFN"
     gene            10881..11288
                     /locus_tag="PLF66_18115"
     CDS             10881..11288
                     /locus_tag="PLF66_18115"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HMZ66011.1"
                     /translation="MYLIDTNIVSELSKPKVNEGVKKWISLQEQLSLSIISYHELRYG
                     INRLKQEQKKKLEAWWNEFLAFSPEFLPIDLNISNIASLIRSKSESEGVKMTLADSMI
                     AATALQHNRILVTRNVSDFEKCGIGLLNPFEII"
     gene            complement(11349..12785)
                     /locus_tag="PLF66_18120"
     CDS             complement(11349..12785)
                     /locus_tag="PLF66_18120"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010576981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HMZ66012.1"
                     /translation="MVFTPSANDLLSILPTLLLSGVGLFFLLIQFIYHSNDHRVIRYL
                     TGISLLVALYFVSFNYHTKPGQGLFFNNQIAVNDITLWLNVIYMITAFLTVVASPRIL
                     NQHGITFPEFYPLILFAVTGMYFMTSGADLIVIFVGLELLSISLYVLIGMAHNEMSSL
                     EATLKYFLLGAFSSGFMLMGIAFLYGASGSTQLAVAMASLSQAGDAGLFAKIGLGLFL
                     VGVCFKVALAPFHSWTPDVYEGALTTITGFMASGPKAAAMGLMLIIFQYLPSSETPSV
                     WVYTIGGIAALSMTLGNVFALKQENLKRVLAYSSISHAGYVVAGIVCGAKMEVVYYLM
                     MYSFMNIAGFAMIAYLENGKYIITYNSIKFLVSKKPLTSVGLLAIFFSLGGIPPLGGF
                     WTKLFLFQKMAESDQFINRFLLIVGVINSAIAIYYYLRVTVSAFMTEEKGDAASEPLS
                     PSFGLSFATVVSLLFVLFSWILFQPNSL"
     gene            complement(12787..14493)
                     /locus_tag="PLF66_18125"
     CDS             complement(12787..14493)
                     /locus_tag="PLF66_18125"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HMZ66013.1"
                     /translation="MPESILSLVIFSPLLGIAILSFLKEEEQIKWTSAIVTFITFVIS
                     APLLWHFNTANAGLQFVHRIPNWMSSGKLVIDYHFGLDGVALLLFVLTSFLFFISSVA
                     SWTYIHKRLKEFYISLLLLEIGVLGVFAAANLVLFYVFWELMLIPMALLIGIWGGENR
                     LYAAVKFFIYTMAGSVLMLAAILIIYFKTGDITIETLSTKALNDFSPSLQAFLFFAFA
                     LSFAIKIPVFPVHTWLPDAHTEAPTAGSVILAGVLLKMGTYGFIRFCIPFFPAVSLEY
                     QGLIMLFCVIGIVYGALVAMVQADGKKLIAYSSVSHLGFCLLGLMTFTEEGVLGGMIQ
                     MINHGVSTGMLFLMIGMIYERTHTRMIADYGGIAKVVPLFAIFFMIAMLSSVGLPGMN
                     GFVGEFLVLLGTLKVNYILGIIAGTGVVWAACYLLWFTKRFLFGDITNKENELLSDLN
                     AREIFVLIPMVILIFWFGVYPQTFMKYLEPSARVYLNSASVQAIAERNNQEQGKKLVL
                     TENYNSLGKAPATFEERLSGFTGHYALLNVNPKLIPIPPTSQEPEPTPKPSEPLKEEE
                     KK"
     gene            complement(14496..16442)
                     /gene="nuoL"
                     /locus_tag="PLF66_18130"
     CDS             complement(14496..16442)
                     /gene="nuoL"
                     /locus_tag="PLF66_18130"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004458581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HMZ66014.1"
                     /translation="MLEYFPLVVLLPLIGFLVNGIHYRKMPNTAAAMIGTSAVLIPFF
                     ITLGSVIAYQPMTNKTPHLFTLLPWIHAGAFQVDFAYQVDQLSLYMTLIITGIGSLIH
                     IYSAGYMHGEDGFNRFFAYLNLFIFSMLNLVLGDNLVLMFLGWEGVGVCSYLLIGFDY
                     HKSSAANAGMKAFITNRIGDVGFAVGIILTYWYLGSVKYVDIMAAMPAALPFKEIINY
                     VALAFFIGAIGKSAQIPLYVWLPDAMAGPTPVSALIHAATMVTAGVFMIARLNPIFLA
                     ADVTSNYIAITGAVTAFFAATIGLFQTDIKKVLAYSTVSQLGYMFLAMGVGAYEAGMF
                     HLMTHAFFKALMFLGSGSVIHAMHHEQDMRNMGNLKSYMKVTWITFMIGTLAISGIPP
                     FSGFFSKDLILEKAFGHHGIGHVIWAIGLAGAFCTAFYMFRLVFLTFYGKERIDHHVK
                     EHLHESPWTITLPLVILSIGAAGAGLIQMPHLFFGGTHFLTDYFQPIFAPGKEIVEGA
                     WKITAEPHHMSESTELILLVVSICVALSGIILSYFVFQVKNSVPPADSGFAGFPKVVY
                     NKYYVDEIYEATIIKPLIRMSNWLSVSVDKALIDGMVVGVGKFFVHISDIFRKIQTGI
                     VGDYALSIVIGTIAILVVLLYGGF"
     gene            complement(16447..16767)
                     /gene="nuoK"
                     /locus_tag="PLF66_18135"
     CDS             complement(16447..16767)
                     /gene="nuoK"
                     /locus_tag="PLF66_18135"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017852550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HMZ66015.1"
                     /translation="MQTYISGIPLNYYLSLAGILFSIGVLGVLTRRNAVIIFMAVELM
                     LNSVNLVFIAFSKVLSIVNGEVIVFFVMAIAAAEAGVGLAIVIAIHRQKKTSNVDEIN
                     LLKW"
     gene            complement(16777..17361)
                     /locus_tag="PLF66_18140"
     CDS             complement(16777..17361)
                     /locus_tag="PLF66_18140"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016547430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HMZ66016.1"
                     /translation="MLNLTEVQPILFIILSTVTVGSAILVVINKNPVASAVFLVLTFF
                     ALAGIYALMNAIFIATMQVLVYAGAIMVLVVFVLMLLSLREETHQEIWSNPPKKTLIL
                     LIVFSFAFLLLASLRTTKEAEPTVITNITSPNYEYKLKDTEESKVIVSGNTASVGAST
                     FIDYLLPFEIISILLLVAVIGAVIIAKKDSVAKS"
     gene            complement(17364..18434)
                     /gene="nuoH"
                     /locus_tag="PLF66_18145"
     CDS             complement(17364..18434)
                     /gene="nuoH"
                     /locus_tag="PLF66_18145"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012388293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HMZ66017.1"
                     /translation="MDWTLILIWALKSGLLFFIIITGCAYYTLAERVVSAFIQDRRGP
                     NRAGPAGLLQPLADGIKFLTKEEVFPVNVNKVMYLIAPAISIVCAILAWSIIPFGGTI
                     PLPPLLANAVGFPSIDLQVANPNTGILFMFAISSLSVYGIILAGWSSNNKYSLLGGIR
                     STAQMISYELPLGLSVVIVILLSGSLKLTDINDAQKGLWFIFTLPGLIAFFIFCVAMF
                     AETNRLPFDLAEAESELVVGFHTEYGAFKFALFFIAEYMNMITMSCVVTLLFFGGYNV
                     PFGILEGSELQPLVGLLFFTAKVLFFAFLFMWVRWTVPRFRYDQLMVLGWKKLIPWCL
                     FNIVLASTYVVYWGKYWKGIFN"
     gene            complement(18437..19699)
                     /gene="nuoF"
                     /locus_tag="PLF66_18150"
     CDS             complement(18437..19699)
                     /gene="nuoF"
                     /locus_tag="PLF66_18150"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016547608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HMZ66018.1"
                     /translation="MAELKLLTKHRGEKDTHTLAHYKSVGGYAALKKALSMKPEDIIT
                     EVKNSGLRGRGGAGFPTGMKWSFIPKVDKPKYLLCNADEGEPGTFKDRVLLEEFAHQM
                     IEGMIIAAKAIDSHQGYIYIRGEYHLSYDRVQAAVDEAYAAGLLGKNILGSGFDFELG
                     LYAGAGAYICGEETALINSLEGRRGHPRLKPPFPAVAGLYGCPTVVNNVETFSAVPHI
                     IQNGAAWYAKMGTPKSTGTRLFSVSGPVKNPGVYEIELGTPLMSLINDLCGGMADGKK
                     LRGIIPGGSSVPILTAKECETANMDFESMAEHKTMLGSGAVIVLAEDTDIVESTYRLA
                     EFYAHESCGQCTPCREGTHWVADLLHKIKEGHGTKKDLELILSLTVNMEGGTTICPLA
                     DACVGAVRPTIQKYRPEFEARLKKEDAA"
     gene            complement(19702..20181)
                     /gene="nuoE"
                     /locus_tag="PLF66_18155"
     CDS             complement(19702..20181)
                     /gene="nuoE"
                     /locus_tag="PLF66_18155"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002973725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HMZ66019.1"
                     /translation="MAYQFSEASEKRFQKLVTMFPDKRSLILPALYLLQRDKGFVDTE
                     GMEYIAEKIGKPIAVSHVYGVATFYTLYNKKPVGKYHIQICANISCYVMGSDKITDHI
                     CKKLGIEKGETTKDKKFTVDEVQCLGACGYGPMAQINENYHEFLTPEKVDEILKTLE"
     gene            complement(20184..>21189)
                     /locus_tag="PLF66_18160"
     CDS             complement(20184..>21189)
                     /locus_tag="PLF66_18160"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020772302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HMZ66020.1"
                     /translation="NQFLVCTDRMNYISTPLNNIGWILTVEKLLQIEVPERVTYVRMI
                     ISELSRIMDHIICNGILGVDLGAFSGMLHLFHHRENIHSILEKLTGARLTTTFCRVGG
                     MERDIYPAFVKEVKEVINGLKPAMDEFNTLLIRNKIFNERTAGVGGISAEDALAFGFS
                     GPNLRAAGVDMDVRKDTPYMLYDKVDFDIPIGEDGSVLHRTLVRMEEMRQSIRIIEQL
                     IDGIPEGPYHANLPHVFLPEKHRVYNNMEELIYHFKLIMHGIKVPPGEYYMATEGGNG
                     ELGFYVVSEGDKSPWRVHVRRPCFWYYQAFSHMVKGQLIADSIATMSSLNVIAGELDC
                     "
BASE COUNT         7111 a         4045 c         3626 g         6407 t
ORIGIN      
        1 cagaattgca acaatagaaa tctctcgacc agaagcgtta aacgcattaa atagagacct
       61 cctattagaa ttagaatctt cgattgccca tttagaagaa gacaaaacaa ctagagtagc
      121 aatcattact ggtgcaggaa aggcatttgt tgctggtgct gacattgcgg caatgaaaga
      181 atatgattat gaaaaagctt acgaatttgg cggaacagga caagttgtat ttactcatat
      241 cgaaagatcg cctatcgttt ctatagcagc gatcaatgga tttgcacttg gtggaggatt
      301 agaactagca attgcttgtg atattcgtat tgcttccaaa aatgcaaagc tgggtttacc
      361 tgaagttgga ctaggactta ttcctgggtt tggaggaaca cagagattac caagattaat
      421 tggtactggt cttgctaaag aaattatatt caccgctgac atgattccag cagaagaggc
      481 ctatcgtatt ggacttgtaa ataaactagt tgaaccgaca gacttactca caactgcaaa
      541 cacaatggca aactctattt gctctagagg atcgcatgct attcgagaag caaaaagagt
      601 aatttgggaa ggtttagaaa ctacacaaga cgaaggaatg aaattagaac aaaaggcttt
      661 tggaaatatc ttcaacgata aagaaacgca tgaaggctta agtgcatttc tagagaaaag
      721 aaagccaaat ttttagataa atgaacgcaa gtccgacgta aaagaaaaat taaaagtttt
      781 ccaccgataa aagatacgat aattcaaaat tatccatcgt gtcgtcatcg atcagttatc
      841 ggtgcgatgc ggtgtggatt cgacaagctc atcaaccaag cgttgtcgaa cagtgcatcg
      901 gtggtgaaaa tatttttaca tccaactaac gttaaattat atgctgcttt ctaaatttag
      961 tctttattct ctaatgtttc agcaatgatt tttactttct gttcaagttc atttatcttt
     1021 gatagaatac tttgaacaga atttgattgc tctttagtag ttgcgctaat ttcttctgta
     1081 gaggatgcag aatcttgaat cacagtaaga atctttcttg agttctcaat tgaatttact
     1141 ttcaattctt ccatagactc gatttcggaa agtagcgttt cattattcct tgcaacttct
     1201 gatactgttt tttcaatttc attgaatgtc tggactgttt gattcatatc agcttcagaa
     1261 gacttgagta aagattcagc ctctaacatc tgctgtttgg ctgaatgaat aaatcgattg
     1321 atttctgtga caatagtaga aatttctttc gttgatctgg aagattcatc tgctaatttt
     1381 cctacttcag aagcaacaac ggcaaagcct ttaccactat ctcccgctct agctgcctca
     1441 atcgaagcgt taagcgataa aagatgagtt tgatctgcaa tcgatttaat tttttctata
     1501 atagaagcaa catctctcga tctagcagat aaatcattga tagcatcatt tacatgcttc
     1561 gttgcttctt gatttctatt aaaagtatcc tttagatttt gaatttgttt taatcctaaa
     1621 agattttcct ttttcatttc tttggatttc tctattaaaa tatttgcagt ttgaagcgta
     1681 gcattgatcc gatctcctaa cttcaaagaa gaatccactg tattatccgc aagctctgtc
     1741 tgtttacttg caccctctgc gatgtattcc agagagttag atacttcatt tgtgctgttg
     1801 actatatcat tgtaagattc tcttacagag gaagtaactt gacttaaatc ttgaatcata
     1861 tttcgaatta ctttgtcttt tccttctaat tgttcttcac tgtattttgc aagcgcccta
     1921 ttctgataaa aattttctag gactctctgc ctaaaagtat atgcccaaaa acaagaaatc
     1981 acaacttgaa aaattgcaac agcataatga aaaaaagctt tagtgaagct gatataggaa
     2041 tcctcaaaaa aatacatttg ccatccagcg ttatgtagca agataaaagt tagatgttgt
     2101 gcagatacat agattgccat tggaacaata ctcaaccagt ccatgtatat aatcataatt
     2161 gcagtggacg taaaaaagaa aaaatgcatt tccgccaatc catgcatctg atagatatga
     2221 agcatcataa aggtttgaat cacgatgccc gcattagatc ttgcaaagcg tgtatctgga
     2281 taaagcttca caacaagata ataggctaga actgcaggaa cagctacgcc tatcgttaca
     2341 agccatgtat cagaataaaa aacgagtata aaagataaga gtagatgaaa taaaatcatg
     2401 gcttgcatga tcttctctgc tttcctgtaa acaggaagca tagcttcatt caactcttct
     2461 ttggttacag tctgtttaat tttctcaata tcaagtttaa gcatacttta aaaaatggaa
     2521 aatagatcgt ctaaaaattc aattctaatt tgattcaaac cctctgatgg caattgacat
     2581 ccgtatgcta ttttagcata tacaggaaga tcctctaatt tctcttcgcg taacaaggac
     2641 tgaagagcaa gtctcgcgta ttcagatgat ttaagggaac agtatcttga tttgttataa
     2701 tttccacgat aatataaatt ccctttcttg tctaaaatga ctgcctgtgg cgttgaataa
     2761 accccataac gatccgcaat ttctcctgtt ttatcttcga cataaggtaa gtttaaatcc
     2821 atccttttaa atttttccag agcattttct ttttctatct gcaaaacaat ggcaaagtcc
     2881 actttatcag aataattcaa aaccaaggct ttaaaatgtt ctaaattaaa acgagaacaa
     2941 gggcaatgcg gattaaaaaa atgaattaaa agcggtttgt ctcttactga aagaattgga
     3001 ttcggcggaa caatagtaga tccaattgga atattttcat aatttgtagg aataggagtt
     3061 ggaagtgaat agcgtgtatc ctctacataa atgacatagg cagaaatact cgctaagaac
     3121 aaaagactta gaatcacaaa tattcgcttt atcatacgtt gatagattgc gttagccgcg
     3181 atggaattaa aaatgtatat ggatgtctag ctttgttttt agacaggatc tctttttcaa
     3241 tctcagctaa ttccttttgg aaattcttta aaggtttttc ttttagagag tctttaaacc
     3301 aatagaaagg atactctccc aatttcgtaa aataaatagc accaagtgta tagcctaaat
     3361 tcaattgtag agatgcgatg tctagggtag gtaacatttt aatccagtct ttttctgtat
     3421 aactttgatt tgccttggga gcttcttcat aaagcgctag cggcacttgc ggcgcataaa
     3481 tcattaaatc tttctgtggg aagttaacgg atgagtgacc agcactagca gtaaaaatga
     3541 tttgcgtggt agctaatacc aaataatcta aagtctctaa tttaccattt ccatcttctc
     3601 caaatccttt cactcttcca cctgataaag aagaaatttc acccgcccaa agttgaagct
     3661 cataatcttt ctttatatca atagggctca agtaaaattt ctccaaataa gatctgaccc
     3721 atttttcaat tgctccccaa accattaaac catcgtctcg gtatggatag tattctaaaa
     3781 ccgcactatt ctctacattt ctttttctaa gagaaaccgg aaagaaagaa tcattgaatt
     3841 ttaaatctaa tactgactcg accgctactc ccgcatctga tgcgatagtt ccagagagta
     3901 gatagtctac tccaccaccg ggtgcaatta actttgcttt agcgaggtaa ttaataaaaa
     3961 tagtcccttg aaaatgagga actataagtc gatagagagg atgtctattt gataattgtc
     4021 tataggttgc gacaacaata ggctctaaaa acaaatgtgt ccttgctaga tgcgttacta
     4081 actcatgatg atttccatct gctgaattta ttacagcctt tgccattttc caagcccact
     4141 cagaggattt aggtgtaaat atcggatatt tctttggatc ttgcccacat tgaattgcaa
     4201 tcggtcttag cttcttctca gttttatcta tagcaaataa cgccattggc gcataacaaa
     4261 acttttcccc attaggctgg taaccgtttt ccaaatgttc aagcgcttta tagtttgtta
     4321 taaaaagtct tttttctcga attgccgatt gaatggaatc gtttttaaat aaaggtaaac
     4381 ttttaaatag tgaatcagta aaaggaaaat tttttggaat tttatctaaa ccctcgatta
     4441 cagtaggatt ccaacctgcc acgcgcataa tcgcaaagtt ttcatctgat tgaaaatcaa
     4501 tatcttgttt gggaatagga atagttccaa acaaatcatt gtattctccc aaatcaattc
     4561 cctcaccggg tttcaggaac tttacaatat aagttcctcc agctacaaaa aaattggtcg
     4621 gatctcccga aaaaagcaga cctaataatc gaaagagcat tttgattcta aaaatttctg
     4681 gaataaaaaa tttcttaaaa ttccaagctg tcttttccct agcggctatt gcatttttta
     4741 caagtgcaac acctacttgc aatagtttaa agacccaaat gacagaagga tattccgatt
     4801 taggtaaacc atctacaatg gcgagaggcg gaataatatt atagttgtat ctatactcgc
     4861 ttctcttttt ttctaaatct ttatttctac attttaaatc tttctcattt tgaggaaggc
     4921 aaatctttgt tatccacata atcgattcct ttataaacta gtcttgatct aacacgattt
     4981 tgccttttag accagaaggc atcataacac caaagaaaaa tgactgtttg tatttcgcat
     5041 ctttctccgg ttcaaatcta gctcttgcat aaaaagaagc aatcgcagtg cgaagataca
     5101 taagtgcaaa aggcatgccc atgcaacttc taggtccacg accaaaaggg aaaaaataat
     5161 cacttccata gggattacat tttataacat ctgacgtcca acgttctggt ttaaatgcaa
     5221 gcgcatcttc ccagtgctct tcggttctat gtaagaaata attggaaaga atgattggag
     5281 tatttgttgg aattttatat tctctatctt tttgtttatc ttttaaaaat aatacttgcg
     5341 tagtcgaact atttcgactg taaaaaggaa ctgggggaag aagacgcatt gtttctcgta
     5401 ttactttttc taaaaattcc attttatcta aatcagcgaa acgaatttct ttgtttttgc
     5461 agattgattt aatttcctgc tttaattttt cttcatactt tttattttca ggctggctca
     5521 aaagccatac gacagttgca aagttactag taacagaata tacaccacca taaaaaatgt
     5581 cggcagtatt tgcagtgagt gctacatctg ataaattcgc tccctttcca gaagtccatt
     5641 gaatgagatc taaagcattt ggatttagtt cattctctga tgtagaaatt aatttttcaa
     5701 agtaacccaa ccattttttt ctttttaaat aaaagaaggg atttaaaaca aatgaaagta
     5761 taaaaggatt tacaagacgc ttcgagcctg tctttccaag cgaaacaaaa agcttataaa
     5821 tacagtcagg aaaatatttt cctgcaatag ccaaagaaaa agaatcaaaa gaaactttct
     5881 ggaaagtttc tgtcaaatca agactcttgc ttctagactt tgcagctaac gcaagaattg
     5941 tagtagacat tcctgaactc aaaatttctg gttgttttgt taaccaatcc tgaaaccagt
     6001 cggaactgaa aggattcttt gctcgcatat tttgccattg aggctggttc gccagaaagg
     6061 gagtgtattt tgtaataaca ggatttaatt gtttgagggg agagtctttg tgaaaatttt
     6121 cccaattcga atccaaaact tctctaaaaa aatcagggga attgatcacc aactgaggat
     6181 tggataaaaa ccaaaggacg taaatttttc catagttctg cgccaagcca gaagtaactt
     6241 cccatggatt ttgtcctaat aatagattta aatttccaag tgggaaacta ggtgtaggtc
     6301 ccggaaatat ttcgaactta gtcattttat ccttataaac gaatgcgaac actttttgca
     6361 aaaaactcat tacaacctca acctttaaat caaaaacttt ctcaaatttt ttttcaatac
     6421 tatatttcta aaattttttc ttcctgccga gaaagctgaa agatttttaa attagtttcc
     6481 tatttattga aaatagttat agttactact gatacaattc agtcaatgca taatgaaaag
     6541 aaaattagaa attcaaatat ataaagaatt tcatcgatca aattggaaat gacttacgct
     6601 tcaaaagaat tttaaaaaat taactttaaa ttttttttct ttctaactga gccactttac
     6661 gcaaaattta taaatctagg aataaacgat gagttttata atattagaga atactaatga
     6721 tctaacaaaa tctatagcct gcgatagctc ggctgttgga aagcttatga agcgatggga
     6781 ggacaaccct ccgctccttc tctctcgcaa tgcccaaacc tattgtcatt ttttaatgac
     6841 aataggagca aacctcgttt cactgcttgg ttcactttgc ttaaggtgag ttaccgacag
     6901 aaagttcaat tcctctagga gatcaaatag ttttcaaaaa atgaattgta acttttaaac
     6961 taacacttgc aatatctatt agagggaaaa taatgaaaca tttaatttta atgatatttg
     7021 cgttagctac ttgcggaaaa aaggaagaac aaaacttaca aactaaggga tctattcttt
     7081 ttgctgtagg aaatgtaaca gtagtatcta acggtaataa aaattctgca aaaaagggaa
     7141 ctcctatttc agaaggagat acaattctaa cagattctaa gtctacggta atagtagaat
     7201 tttcagacaa tgctggaaca attgaaattc aagaaaactc tgaatttaca gttaaagaat
     7261 tcaatgagaa aaaccaagaa cttgtatcta aaagtggaag tttctggttg aatgctaaaa
     7321 aactaaaaaa agatacaaat ttcaacgtga ttatgcctac atcagtagct ggaattagag
     7381 gaacaaagct ttactcgttt gaaaataaag aattggggat aacaggagtt tgccactgtc
     7441 aaggtgcggt tgaattcaaa gagaactcat ccaattatac aggcagtcat gatagtgatt
     7501 ttttagtttt aacaaaggct ggtaagacaa tcgtgattac tccggaagat tttcaaaaaa
     7561 tgggttacca aggtagtgat gacaatcacc aacacagtag tctagagaat agtccacttg
     7621 gtaaaaaagc taatctagat tcagaagctc accgaaaagt tttagaaatt gcagataaaa
     7681 aatggaaaga gaaataaaag aataaattaa aataaaaaat gccaccctta acaaagagtg
     7741 gcattctgaa attcttacag attgttttgt aaatcttaaa cgataaacaa catctcaccg
     7801 taagttggaa ttggccaata agtagaatca acgatcaatt ctaatttatc agctgcttct
     7861 ctcactaaat tcatctttgg aatcactgaa tctctgaagt gttgcgcatg ttttaataca
     7921 tctgcatctt catgatctaa cgctttttct aaatcatcta atttagcttt tagagtttca
     7981 ttgagactag caattgtctt taatgtttca atttgtcctt tcacagcacc atcacctaga
     8041 acagacttag tatcagcaat agctcttgct aaatcacctt ggtatttgag agtagctgga
     8101 agaatttgtg ttctacccat acttaaagta cactgacctt cttggttgat tactttattg
     8161 taaccttcta gaataatcgc ataacggctt tctacttctc ttgcacttag aactttgtat
     8221 ttttcaaata cggcaatgtt ctcaggctta atcatagaag gtagagcatc taccaatgtt
     8281 ttgaaatttg gtaaaccgcg tttagcagct tcgtttttcc attcatcagt gtagttatca
     8341 ccattgaata taatacgttt atgctttttg atattcgcag ttaaaacttt ttgaacacct
     8401 tgctcgagtg ttgaaccagc tttgatttca ttttctaact cggtagccat ttcatctaac
     8461 gcctcagcaa caaatgtatt gaggaatgca tttggataac caagagaaag agaagaagga
     8521 acagcacgga attcaaactt atttccagta aaagcaaacg gagaagttct atttctatct
     8581 gttgtatcct ttggtaaaga tggaagagta gaaacaccaa tttccataaa tccaccttta
     8641 gaagatgtag ttgcaggacc tttttcgatt tgctcgatga tgtctgtcaa ctggcttcca
     8701 agaaagattg agataatcgc tggaggagct tcgttcgcgc caagacggtg gtcatttcct
     8761 gcattggaaa tcgcaactct taagaggtcg ccatgcttat caaccgcttt gattgtagca
     8821 actaagaaga ataagaattg tgcattgtcg tgtggattat cgcctggctc aagtaggttt
     8881 tgtccatcgt tagatgacat tgaccagttg ttgtgttttc cagaaccgtt tacacctttg
     8941 aatggttttt catggagaag acaagcgagt ccatgtttac ctgcaacttt acggagtgta
     9001 tccattacga tttggttatg gtccattgct atgttagttg tgctaaaaat tggagccatt
     9061 tcatattgag ctggagcaac ttcattgtga cgtgtttttg cgttcacacc aagaagccaa
     9121 agttctgctt ctaattcttt catgaattcg aaaattctag gtttgattga accaaaataa
     9181 tgatcttccg attcttgccc ttttgccggt ttcgctccga ataaagttct accagtaagg
     9241 attaagtcct gtctcatgtt gtagaaattt ctatcaatac agaaatactc ttgctcagga
     9301 cctacagttg tagtaacacg agtagcagtt ttatttccaa agagtcttaa tactctaaga
     9361 gcttgctcag ataaagcatc catagaacgt aaaagaggag ttttcttatc taaagcttct
     9421 cctgtataag aacagaaggc agtcggaatt acaagggtag tttcacgaat aaaagctgga
     9481 gaagtagcat cccatgctgt ataacctctt gcctcgaatg ttgctctaat tccaccagaa
     9541 gggaatgagg aagcatctgg ttcgcctttg atcaattcct ttccactgaa ctccataatt
     9601 acttcgccta cttcttgtgg gctgataaag gagtcatgct tttctgctgt taagccagtt
     9661 aatgggtgaa accagtgagt atagtgagtt gcaccatttt ccaatgccca atctttcata
     9721 gcattggcaa caatgtcagc aatttgcgga tctaaaggct taccttcaaa aattgtcttt
     9781 ttgagagatt taaaaacatt tttaggtaat ctatcttgca ttaccttaat gctaaatact
     9841 ttgcttccaa agagttctgg gaccggagta tctgcaaaat cgtaaccaga atcctctggt
     9901 atatagtctg taattctaga aattgcttct tgtctagcag ttctgatatt cataattttt
     9961 ccttaacctt gtattgtgat actgttttca ctatttttcg atgttctatt tttgaaaatg
    10021 atattttaaa aatagaaagc cgaaataatg gtattctatc ttaattttaa agcaaatttt
    10081 atgccactta ttcgtattta gtaaatagtt taagtaaaaa tagtgttaaa tgattgtaga
    10141 ctcaagtaaa gcattgtctc aacaacaatt gaccgagact tagaagtaaa attgaaacct
    10201 tgctcctttc tattgcgaaa aaccttttca ctcgaactga aattaaaatc aatgtccccc
    10261 gagaaagtaa atatcttcat ctagattttt acctaatttt ttgagataac ggcttacatc
    10321 ctttataaga tttagtattt agcaactcat gtagcgaatg agtgaatatt gctgtaaatc
    10381 agaacacaat caaatcattc tatttgagag gaagatagag taagcaatcc tatatagata
    10441 aaaaatatca tagaattgct tagcaatctt taaaaaatag ttgaccatgt aaaatcattt
    10501 caacaaacta ttatagtcat ctatttatga tcttaagatc cataagtaaa aattcaattc
    10561 aagatataga aacattatag tgattcattt tcaatcaata gagaggaggc cttaaatggt
    10621 ttggaaaatc gcaaatgcaa aatcccaatt ctctgaatta atccatcaag cctgtgttga
    10681 gccacagttc atctataata gagaaaaacc ttcagcagtt gttataagtt tcgaggaata
    10741 caatcgactt cgaaacctag atatactcta ccacaaacca ccaaagtggg caaattttgt
    10801 atcgtattct gagaaattga ttcaggagac ggatttgtcg gatggactag aattgccaac
    10861 cagagacgat agagagattg atgtatttaa ttgatacaaa tattgtttca gagctttcta
    10921 agccaaaagt aaacgaagga gttaagaaat ggatttcttt acaggagcaa ctctctttaa
    10981 gtataatttc ctatcatgaa ttgagatatg gtataaacag gttaaaacag gagcaaaaga
    11041 aaaaattgga agcttggtgg aatgaatttc tcgctttctc tccagaattc ctcccaatag
    11101 accttaatat ttcaaatatt gccagtttaa tccgctctaa atctgagagt gaaggagtca
    11161 aaatgactct tgccgactca atgattgcag caaccgccct tcaacacaat agaatactag
    11221 tgactagaaa tgtaagtgac tttgaaaaat gtggtatcgg attattaaat ccatttgaaa
    11281 tcatttgaga attcaataaa aactgctaat tatcattgaa tcctcaagat tctttcctgt
    11341 ttcttttatt ataaactatt tggttgaaat agaatccaac taaagagaac aaaaagtagc
    11401 gaaacgacgg tagcaaaaga taaaccaaaa cttggtgaga gtggttcgct tgcagcatct
    11461 cctttttctt ctgtcataaa tgcagaaaca gtcactctta agtaataata gattgcaatt
    11521 gcagagttga tcactcccac aatgagcaag aatcgattga taaactgatc tgattcagcc
    11581 attttttgaa ataaaaacaa cttagtccaa aatccaccta atggtggaat accacctaac
    11641 gaaaagaaaa tagccaaaag ccctactgat gtaagaggtt ttttagatac caaaaacttg
    11701 attgagttgt aggtaatgat atactttcca ttttccagat aagcgatcat tgcaaaccct
    11761 gcaatgttca taaaggaata catcattagg tagtaaacga cttccatctt tgccccacac
    11821 acaataccag caactacata accagcatga gatatggaag agtatgctag aactctcttt
    11881 agattttctt gtttgagagc aaacacattt ccaagagtca tggataaagc agcaattcca
    11941 ccgattgtat aaacccaaac acttggtgtt tcactactag gtaaatattg aaaaataata
    12001 agcattaaac ccattgcggc tgcctttgga cctgacgcca taaaaccagt aattgttgta
    12061 agagctcctt cgtatacatc tggagtccaa gaatgaaacg gagccagagc caccttaaag
    12121 catacaccta cgagaaataa accaagacca atctttgcaa aaagacctgc atcgcctgcc
    12181 tgggaaagag aagccatagc gacagctagt tgagtgcttc cacttgctcc atataagaat
    12241 gcaattccca taagcataaa gccagaagaa aatgcaccta aaaggaagta tttcaaagta
    12301 gcctctagac tactcatttc attgtgtgcc attcctatta atacgtaaag actaatcgat
    12361 agtaattcaa gtccaacaaa gattacgatt agatcagcac cacttgtcat aaaatacatt
    12421 ccagttacgg caaaaagaat caatggatag aattcaggaa atgtgattcc atgttgattc
    12481 aaaattctag gtgaggcaac tacagttaaa aatgcagtta tcatatagat aacatttaac
    12541 caaagagtaa tatcatttac agcgatttga ttattaaaaa acaaaccttg ccctggctta
    12601 gtatgatagt taaaactaac aaaatacaaa gctaccaata aactaattcc tgttagatat
    12661 cgtatgactc tatgatcatt gctatgatag ataaactgaa tcagtaggaa aaatagacct
    12721 acaccactca agagtagcgt tggtagaatg gataataaat catttgcact cggagtaaaa
    12781 accatattac ttcttctcct cttcttttaa tggctcactt ggctttgggg ttggttccgg
    12841 ttcttgactc gtgggtggaa tcggaatcaa ttttggattt acattgagta acgcataatg
    12901 ccctgtaaac ccagataatc tttcttcaaa agtagcagga gctttcccaa gtgaattgta
    12961 attttcagtt aaaactagct ttttaccctg ttcttgatta tttctttcag ctatcgcttg
    13021 aaccgacgca gaatttaaat aaactcttgc actcggttct aaatatttca taaatgtctg
    13081 tggataaaca ccaaaccaga aaataagtat taccatcgga attaaaacaa aaatttctct
    13141 cgcatttaga tcagatagaa gctcattttc tttattggta atgtcaccaa ataaaaagcg
    13201 ttttgtaaac caaagtaggt agcaagcagc ccaaactact cccgttcctg caatgattcc
    13261 aagaatataa tttaccttta aagttccaag tagaactaaa aactctccaa caaagccatt
    13321 catacctgga agaccgacag acgaaagcat tgcgatcata aagaaaatcg caaagagagg
    13381 aactactttc gcgattccac cgtaatccgc aatcatacga gtatgagttc tttcatagat
    13441 cataccaatc atgaggaaga gcattccagt agaaactcca tgattgatca tctggatcat
    13501 tccacctaga acaccttctt ctgtaaatgt cataagacca agtaaacaaa atcctaagtg
    13561 agaaacagaa gagtaagcga tgagtttctt tccatctgct tgcaccattg caacaagagc
    13621 tccatacaca atgccaatca cacaaaagag catgattagt ccttggtatt ctaaagagac
    13681 agccggaaaa aatggaatgc aaaaacgaat aaatccatag gttcccatct ttaataatac
    13741 ccctgcaagg attacagaac cagccgttgg agcttctgta tgtgcgtcag gtaaccatgt
    13801 atgaacagga aaaaccggaa tcttaattgc aaagcttaaa gcaaaagcga aaaataaaaa
    13861 tgcttgtaaa gagggagaaa aatcgttaag cgcttttgtt gatagcgttt caattgtaat
    13921 atctcccgtt ttaaagtata ttatgagaat ggcagcaagc atgagaacag accctgccat
    13981 tgtataaatg aaaaacttaa cagcagcata gagtcgattt tctccacccc aaataccaat
    14041 taggagtgcc attggaatta acatgagttc ccagaacaca tagaataaca ctaagttagc
    14101 cgctgcaaat actccaagaa ctccaatctc taaaagaaga agggagatat aaaattcttt
    14161 taacctttta tgaatatagg tccaagatgc aacacttgaa ataaagaaca agaaagaggt
    14221 taaaacaaat aagagaagtg caaccccatc tagtccaaag tgataatcga ttactaattt
    14281 tccagaactc atccaattag gaattctatg cacaaattga agtccagcat tagccgtatt
    14341 aaagtgccat aaaagtggag cactaatcac aaaagtaata aatgtaacaa tcgcactcgt
    14401 ccacttgatt tgttcttctt cttttaagaa ggaaagaatc gcaattccaa gaagaggtga
    14461 aaatataacc agtgataaaa ttgattctgg catggttaaa atcctccgta aagtaataca
    14521 actagtattg cgattgttcc aatcacaatc gatagagcat agtctcccac aattccagtt
    14581 tgaatcttgc ggaagatatc agaaatatga acaaaaaact ttccaacccc aactaccatt
    14641 ccatcaatga gagccttatc aacggataca gatagccaat tcgacattcg aatgagcggc
    14701 ttaataatag tagcctcata gatttcatct acatagtatt tattataaac aacttttgga
    14761 aaacctgcaa atccagaatc agcaggaggt acagagtttt tcacctgaaa tacaaaatag
    14821 gataaaataa taccgcttaa cgcaacacag atagaaacaa cgagtaagat taactcagtt
    14881 gactcggaca tatgatgtgg ctctgcagta atcttccacg caccctctac aatttctttt
    14941 ccaggtgcaa agatcggttg gaaataatcc gtaagaaaat gagttccacc aaagaataaa
    15001 tgtggcattt gaatgagccc agcacctgcc gctccaatag ataaaatcac gagaggcagt
    15061 gtaattgtcc aaggtgactc atgcaaatgc tctttgacat gatgatcaat tctttctttt
    15121 ccatagaatg ttaagaatac taaacggaac atataaaaag cagtgcagaa agcacccgca
    15181 agtccaatcg cccaaattac atgaccaatt ccatgatgtc caaatgcttt ttctaaaatt
    15241 aaatcctttg agaaaaatcc actgaaagga ggtataccgc ttatcgcaag tgttcctatc
    15301 ataaatgtga tccaagttac tttcatgtaa ctttttaaat ttcccatatt tctcatatct
    15361 tgttcatgat gcattgcatg aatcacagaa ccagaaccaa ggaacatgag tgccttaaaa
    15421 aacgcgtgtg tcatcaaatg aaacattccc gcttcataag caccaactcc catagcgaga
    15481 aacatatatc caagttggga aacagtggaa taagctaaaa cttttttgat atctgtttga
    15541 aataaaccaa tcgttgcagc aaagaatgca gttactgcac ctgtaattgc aatataatta
    15601 cttgtaacgt cagccgcgag aaagattgga tttaatcttg cgatcataaa tacaccggct
    15661 gtaaccatgg tggcagcgtg aatcagtgct gatacaggag taggacctgc catcgcatca
    15721 ggaagccaaa catataaagg tatttgcgct gacttaccaa tcgctccaat aaaaaatgca
    15781 agtgctacat agttgataat ttctttgaat ggaagggcag ctggcattgc agccataatg
    15841 tccacatatt tcacagatcc taaataccaa taagttaaaa taatcccaac tgcaaagccc
    15901 acatccccga tacggtttgt aataaacgcc ttcataccag cgttagccgc agaagattta
    15961 tgatagtcaa atccgataag taaataagaa caaaccccta ctccttccca acctagaaac
    16021 attaatacaa ggttgtcacc tagaactagg tttaacatag agaagataaa tagatttaaa
    16081 taagcaaaga atcgattgaa gccatcttcc ccatgcatat aacctgcact gtagatatga
    16141 atcaaagaac cgataccggt tattataaga gtcatataaa gcgaaagctg gtctacttgg
    16201 tatgcgaagt caacttgaaa agctcctgca tgaatccaag gcagaagagt aaataagtgc
    16261 ggagttttat tagtcattgg ttgataagca attacagaac caagagtaat aaaaaacggg
    16321 attaaaacag cactcgtccc aatcattgcg gctgccgtat tcggcatttt acgataatga
    16381 attccattga ctaaaaagcc aatgagaggt aataaaacta caagtggaaa atattctaac
    16441 atagtgctac cattttaata gattgatttc atcaacatta gaagtcttct tttgtctatg
    16501 aattgcaata acaatcgcaa gacctacacc tgcttccgca gcagcgattg ccattacaaa
    16561 aaaaacaata acctctccat tgacaatgga taaaactttt gaaaatgcta tgaacacaag
    16621 attcacagaa tttaacatca gttctaccgc cataaaaatt ataactgcat ttcgtctcgt
    16681 aagtactccg agcactccga tggaaaataa tatacctgcc aaagacagat aataattgag
    16741 tggaattcct gatatatatg tttgcaaatt ctccccctaa ctcttcgcta cactatcttt
    16801 ttttgcaatg ataacagccc caatgactgc aactaaaagt aagatagaaa tgatttcaaa
    16861 tggaagtaag taatctatga aagtagaagc tcctacactg gcagtatttc cactcacaat
    16921 tacttttgat tcttctgtat cttttaattt atattcataa tttggagaag tgatgtttgt
    16981 aatcacagtc ggctctgctt cttttgttgt tcttagagat gcaagtaata aaaatgcaaa
    17041 agaaaaaaca atcagaagta tcaaagtctt cttaggagga ttgctccaaa tctcttggtg
    17101 cgtttcttct cttaaagata aaagcattaa tacaaacact actaaaacca taatcgcacc
    17161 tgcataaaca agcacttgca tggttgcaat aaagattgca ttcataagtg cataaatccc
    17221 tgctagtgca aaaaatgtta atactaaaaa gacggcagac gcaacagggt ttttattaat
    17281 tacaactaat attgcagaac ctacagtcac agtagataaa atgataaata aaataggttg
    17341 aacttcagtt agatttaaca taattaatta aaaatcccct tccagtattt tccccaataa
    17401 acaacatagg tagatgcaag aacgatattg aatagacacc aaggaatcaa ctttttccaa
    17461 ccaagaacca taagttggtc atagcgaaat ctaggaacag tccagcgcac ccacataaac
    17521 aagaatgcaa agaataagac ctttgcagta aagaatagaa gaccaacgag aggttggagc
    17581 tcacttcctt ctaaaattcc aaatggaacg ttatatccac cgaaaaataa aagagtcact
    17641 acacaactca tagtgatcat gttcatatac tctgcaataa aaaagagagc aaacttgaat
    17701 gctccatact ctgtatgaaa acctaccaca agctcagatt ctgcttctgc taagtcgaaa
    17761 ggcaaacgat ttgtttccgc gaacatcgct acacaaaaaa tgaagaaagc aatgagtcct
    17821 ggcaatgtaa aaataaacca aagtcctttt tgtgcatcat tgatgtcggt aagctttaag
    17881 gatcctgaaa gtaaaattac aatcacgaca gaaagcccaa gcggcaattc gtagctgatc
    17941 atttgagcag tagagcgaat cccacctaaa agtgaatact tattattgct actccatcca
    18001 gcgaggatga ttccataaac ggacaaagag gagattgcaa acatgaataa aattccagta
    18061 tttggatttg ctacttgcaa atcaatggag ggaaatccaa ccgcatttgc taagagtggt
    18121 ggaagaggaa ttgttcctcc aaaaggaatg atcgaccatg caagaatcgc acatacaatt
    18181 gagatcgccg gtgcaattaa atacatgact ttgtttacat ttacaggaaa aacttcctct
    18241 tttgtaagaa atttaattcc atctgcaaga ggctgtaaaa gtccagcggg acccgcacgg
    18301 tttggacctc ttctatcttg gataaatgcc gacaccactc gttctgctaa cgtataatac
    18361 gcgcagccgg tgatgattat gaaaaataat aggccgcttt taagagccca gattaatatt
    18421 aatgtccaat ccataactat gccgcgtctt cctttttcag tctggcttca aactctggac
    18481 ggtatttttg aatggtaggt cgaactgctc ctacacaagc atcagcaagc gggcaaatcg
    18541 ttgtcccccc ttccatattt acagtcagag ataaaatcaa ttctaaatct tttttggttc
    18601 catgaccttc tttgatctta tgaagaagat ctgctaccca atgcgtgccc tctctacaag
    18661 gagtgcattg tccacaagat tcatgtgcat aaaattctgc gagtcggtaa gtagattcta
    18721 caatatcagt atcttccgca agaacgataa ccgctccact gccaagcatt gttttatgtt
    18781 ccgccatgga ctcaaaatcc atattggcag tttcacattc cttagctgta agaattggaa
    18841 cagaagaccc accagggata atccctctta gcttcttgcc atctgccatt ccaccacata
    18901 aatcgttgat caaggacatg agtggagtcc caagttcgat ttcatagact cctggatttt
    18961 tcacaggacc acttacacta aaaagtctcg tgcctgttga tttaggtgtt cccattttag
    19021 cataccaagc agcaccattt tgaatgatat ggggcaccgc gctaaatgtt tcaacgttat
    19081 tcactacagt cgggcaacca tagagtcccg caactgcagg gaaagggggt ttcaatcttg
    19141 gatgccctct tcttccttcc aaggagttga tgagagctgt ctcttcccca cagatgtagg
    19201 cacctgctcc tgcataaagg ccaagttcaa aatcaaaacc acttcctaga atattttttc
    19261 caagaagtcc agccgcatat gcttcatcta cagccgcttg cactctatcg taactgaggt
    19321 gatattcgcc tcgaatataa atataacctt ggtgtgaatc aattgccttt gcagcaatga
    19381 tcattccctc aatcatttga tgtgcaaatt cttcgagtaa aactctatct ttaaaagttc
    19441 ctggttcacc ctcatctgca ttgcaaagta aatatttagg tttatccacc ttaggaataa
    19501 aagaccattt cattcctgtc ggaaaaccag ctccccctct tcctctgagt ccagaatttt
    19561 taacttctgt aatgatgtct tcaggtttca tagagagtgc ttttttcaaa gcagcatatc
    19621 cacccactga tttgtaatga gcaagtgtgt gagtgtcttt ctcgccacgg tgttttgtta
    19681 agagctttaa ttctgccatc gttactccaa tgtttttaaa atttcgtcta ctttttcagg
    19741 agtgagaaac tcatgataat tttcattgat ctgtgccata ggaccatacc cacaagcacc
    19801 tagacactgt acttcatcga cagtgaactt tttatctttt gtagtttcgc ctttttcaat
    19861 ccctaatttc ttgcaaatat gatctgtgat tttatctgat cccatcacat agcaggaaat
    19921 attggcacag atttgaatgt gatattttcc gaccggtttt ttgttataga gagtatagaa
    19981 agtagctact ccatatacat gagataccgc aatcggttta ccgatttttt ccgcaatata
    20041 ttccatacct tccgtatcta caaagccctt gtctctttgt agaagataaa gagctggtaa
    20101 aataagagat cttttatcgg gaaacattgt aactagtttt tgaaaacgtt tttcggacgc
    20161 ttctgaaaat tgataagcca taattaacag tctaactccc cggcaattac atttagtgaa
    20221 ctcatagtag caatggagtc agcaattaac tgtcccttta ccatatgtga gaatgcttgg
    20281 taataccaaa aacatggacg tcttacatgc acacgccatg gagatttatc tccttcggaa
    20341 acaacataga acccaagttc tccgtttcct ccttctgttg ccatgtaata ttcccctgga
    20401 ggaactttga ttccatgcat gattaattta aagtgataaa tcaattcttc catattgttg
    20461 tatactctgt gtttttcagg aagaaacaca tgaggaagat ttgcatgata aggaccttcc
    20521 ggaataccat cgattaattg ctctataata cgaatcgact gacgcatctc ttccatacga
    20581 acgagggttc tatgaagaac agatccatct tctccaattg ggatatcaaa atctactttg
    20641 tcgtatagca tgtatggagt gtcttttcga acatccatat ctacaccagc agctcttaaa
    20701 tttggtcctg aaaaaccaaa tgcgagtgca tcttctgcag aaattccacc gaccccagca
    20761 gttctttcgt tgaaaatttt atttcgaatc agaagtgtat taaactcatc catcgcaggc
    20821 tttaaaccat tgattacttc ttttacttct tttacaaagg caggatagat atctctttcc
    20881 attccaccta ctctacagaa ggtagtcgtt aggcgcgcac ctgtaagttt ttctaagata
    20941 ctgtggatat tttctctatg atgaaaaaga tgaagcattc cagagaaagc tcctaaatcg
    21001 actcccaaaa ttccattaca gatgatatga tccatgatac gagaaagttc agaaataatc
    21061 attcgaacat aagttactct ttcaggaact tcaatttgta aaagtttttc tacagttaaa
    21121 atccaaccaa tgttgttgag tggagtagaa atataattca ttcgatctgt gcaaactagg
    21181 aattggtta
//
LOCUS       DAOQOS010000094        21077 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_1220_length_21077_cov_5.427029, whole genome shotgun sequence.
ACCESSION   DAOQOS010000094 DAOQOS010000000
VERSION     DAOQOS010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 21077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21077
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1220_length_21077_cov_5.427029"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(372..560)
                     /locus_tag="PLF66_18165"
     CDS             complement(372..560)
                     /locus_tag="PLF66_18165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66021.1"
                     /translation="MLLVDSAYESFLKKTAIPEHINTTVNCFFKGKQKRREKIYRNEY
                     NVFLAFSTLANNVRLNFS"
     gene            686..1627
                     /locus_tag="PLF66_18170"
     CDS             686..1627
                     /locus_tag="PLF66_18170"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HMZ66022.1"
                     /translation="MKRIYTNLIVIGFLLNTSCMSIIKKEEVGILSSPGTAKVVDTGK
                     VYVGINEELVLYSTRWENHVEKVDVITQDDLHIDVVASIIMRPVKEKILDVHLNIGKD
                     YYQRIVKAEFRTAIRNVMTGYQMIMISKKSQTIEDEIKDLVARKLSAKNLEVSDVNID
                     DINLSPKILQAVEDKIARQQELETMQYDMKIAQRNEEIAKMKAKTESEVEIIRAEKED
                     QIAKIKVKTEAESDIIRAQARAKAQKLVNAHLTPNYLKLKAIESHYKAFESPNSKIIF
                     IPVGKDGLPAYVNINDKMFPNVAEDTKSKKTNNTIEE"
     gene            1704..2600
                     /locus_tag="PLF66_18175"
     CDS             1704..2600
                     /locus_tag="PLF66_18175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66023.1"
                     /translation="MQNIYRFSKSISDEEKIKSPTEYLSREEEFKNFESEIRSQIQNL
                     KPIERISYLNKISQEFGRRIALPCPDVLVAICPYCSRKIWARIRNGLFTLCASFWILE
                     SDDGKWVTEDSKCQHFFLLDGALHLNSKPIPEKLTAYGFVRNKIYMGAEVPFIKPRIL
                     KKEGVIAVIHSIAIGNGYTGYPITYFTKEKISQSEFAMGWAREEYSAKDELVSNATFT
                     GVRFEPQDYDLEKWIQLDKIKWIISQKEDFCLNNPKDFFPYLNLEGRKNPYTIQNGRI
                     LDLPNSTQIEGSANLETFMPGQ"
     gene            2673..2837
                     /locus_tag="PLF66_18180"
     CDS             2673..2837
                     /locus_tag="PLF66_18180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66024.1"
                     /translation="MTIMFLFEVVYFVMKKDSIEREGLILVNRQSSDSNRKMMHHAIV
                     VAKCLAKIAL"
     gene            2978..3640
                     /locus_tag="PLF66_18185"
     CDS             2978..3640
                     /locus_tag="PLF66_18185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010574295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMZ66025.1"
                     /translation="MIQIKNLKKSYSFGNQKLEILKGINIDLPQGKLITLMGPSGSGK
                     STFMHILSGIDRPDSGNVFINGEDISSFSEKDITQFRRKKIGIIFQFFNLMPYLSSVE
                     NVCLPLYLAGIGKKEAYPKAEKALSLVGLKNRFTHKPYELSGGEQQRVAIARSIVNSP
                     EILFADEPTGNLDTENSDHIMELLIQLQKDHGFTIVMVTHNVEIGNRGDIKLSMKDGI
                     LV"
     gene            3637..6153
                     /locus_tag="PLF66_18190"
     CDS             3637..6153
                     /locus_tag="PLF66_18190"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HMZ66026.1"
                     /translation="MNSRLIYLFLIEYFRTHKVKTFFSLVGIVLSVALFVTTTFNGER
                     AEKSLIDFSMGYLGDRYQARIKNLNDKIGITDSDIENLFYSEELRFIDKIIPRIHKTV
                     KLKTNQATLNVIYQGIDIIKEREDLTITSICRNCNRESKTFISSALFDKIGTDSFSFT
                     IESVEYKVTNAAVVDSEGGLFILEDVEDVKERLSQTYYDSILLSISNTTNENLSILKT
                     SLSSYNKNFQLETSEEVKNRASNVLKSFHLNLIIISMISVLIAFFMVSNTMTGIFLNR
                     KRELGILRCLGSSQLENLFLFLSQSVVLGVIGTVLGILLGTFLSKHSFFSGESTLTDI
                     NQSISYTKIPFKIILISSMIGIFGSVLSGLFPALRSFRLAPITIVREDPNQTSVRNFK
                     TLFYVGILFIGVAYLLTQIRSSSNIPIFGLIAIGMIILGQTMCFPQLMKIFLSLANQV
                     FQSLDHSFIETRIGFEEIVHNTYKNTLTSATLMLGVSLIVSLSVLTLSYERSIMNWTE
                     REFPFDFSIINQKDLEEGEDLGVPISLSSKIAKLENVEEVDVFVLNTKAEVGDKIFTI
                     HAYDMELTKIRELKKGIFIHSDINLKEEILVSSNMAYLNHLKKGDEILLNTRKGKKSL
                     RIAGMREHFFSENGTILMDYRLYDELFGIDKFSSIRVNFKDKGNLEDDLERLKQEIAP
                     FPNLKPMNGQELKDIYLNGLKRVFKVLESLKYTAALIAFVSLFSSILYNLNDKLRIFG
                     VFRSIGSSQMQLNQIVFAENLFLTCFGICMGILSSLLLSPIILHVINKTAFGWTLTVE
                     IPYSLLFVCILSIPFVSFFTTLYPYLILKKLSLREILSYE"
     gene            6446..7339
                     /locus_tag="PLF66_18195"
     CDS             6446..7339
                     /locus_tag="PLF66_18195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66027.1"
                     /translation="MSVSNVKILQDIANQIKEENLRGRERIPTSETFIKRMMTLHSKS
                     AEDIKFFLEQLKELHYIFVITVVASDPTLFVQGVDAYVYAELSILNELKHYTEQRLTQ
                     VYESTYYKRKSGFQIIRELLPKIKEFNNTQLGRNLNEAIMLDEYSRLITTSPFEYTDS
                     WRKEKLYKLYREEEPNYEVEYEVEGNSPYPQSTDRRVQDSGNSKWAKAVNQFSIRFLI
                     RIHFRKYEFDVVKRLVLTSKISMLDDLIYIRNTLKDLEKQLDKDTILKYHLDKIIELR
                     RITQAKINNLRKIQQSAAGSV"
     gene            7336..8553
                     /locus_tag="PLF66_18200"
     CDS             7336..8553
                     /locus_tag="PLF66_18200"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HMZ66028.1"
                     /translation="MSRILIVDDDIVILETLKDVLTAAKYNVIASSNPVEALKIYKEN
                     PTLVVITDLIMNEMSGRDLMIKLFETGHKPLVIILTRMNDSKTIIDLFKSGAHDYTIK
                     PFAVSEIVAKVNKAFELAELRIVNENLKREREIRFENQFKWNIFRDNSKNTKSDIHLI
                     DGIKTSFLQGSGIGALVPIIDMIKQSSKEEGEVFLVKKKLLKMLFSNAEFLNRLIEII
                     ADIDHVSNMEIPKEKISIHILHEFIETVINDLDKYAALRNHSVVLTKNDYSNVSRSIL
                     LNKEYFRKALQELLLNAFKFSEEGSKIFILFEVIKEQLFISILNHPDSNGKELNGILP
                     EFQALVFEPFFRISRYVHEEIPTLDFGLGLCFVDRIIRNHNGKIKVGNIKNHTNPNGI
                     LINFTIEIPFAND"
     gene            8546..9613
                     /locus_tag="PLF66_18205"
     CDS             8546..9613
                     /locus_tag="PLF66_18205"
                     /inference="COORDINATES: protein motif:HMM:NF015724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HMZ66029.1"
                     /translation="MIDSLKRIFFITFIFLFFYQCTLHHYEMGRKPIRIVDSLELREK
                     RKMSLDRIPNPIESKKKDNSIASSYNSDGFRDYIKRGCCIEIVEDAKLKIRYSSNVKS
                     DLLYAPTVFLWMFSLGLIPVFEKVDAEIKIEVLDSNENILREYYYNIEEHAYNSWLTI
                     PVSLILISNDSFAHSFIHMFDYPQRFIANKFETDFQEDLAQGKISENINILNQSKDIQ
                     NKSRIAILPIRHQFSKDQMIANAIRDKVETILVNKNFIVVERVKLDEILKEVKLSQTG
                     LTTSEQAQIGSMLNASNLIVGELLDIDRNDSTLEFSLRNVDMETGKILWKYEFSIDEK
                     NSTQSLQEAMKSLDSKIKPMK"
     gene            complement(9616..10683)
                     /locus_tag="PLF66_18210"
     CDS             complement(9616..10683)
                     /locus_tag="PLF66_18210"
                     /inference="COORDINATES: protein motif:HMM:NF040144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HMZ66030.1"
                     /translation="MKSFLSFLFLISFFIVPLLSEDSISENEARTKYRVAILDFKNGN
                     GVTPQEAKDITNIIRTEMINLNTFTILNRENLNKILEEQKNIQLGLCDTTDCNIRLGR
                     LLGANKIMTGKIFKLGRSYHINAYLIDIEKGVEEVAEHETTSSLDGMHLAVENLTLKI
                     REMVLNPDKDRSSLNPSPAYSDTKEDTKELDLGIKPSEVSPYLSFLVPGLGQFMNGYK
                     VKGSLFFISFFGLLSLDLRANHDYHQEKTRYRTANYLVSNSFLRNNTLAYTFMQNEKE
                     DAKKKSDIASAEVAVYSTFLILIYVFNILDALLWSDDFSSLKNPMYEKQNGFFIYSKV
                     EPNSILNKNSSIYSFGYSKEF"
     gene            11144..11497
                     /locus_tag="PLF66_18215"
     CDS             11144..11497
                     /locus_tag="PLF66_18215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66031.1"
                     /translation="MKKLFLIFIFMPILLESTDRKNSTTNWTEYLGIMNWNEANQKCK
                     KLGMRLPSRSDWLEPYNSDQRKSWRKPNGSWFSVYWTSEEVSGAGAYVFSLEDGDPDR
                     YAKDMKVHVRCIRLK"
     gene            11644..12861
                     /locus_tag="PLF66_18220"
     CDS             11644..12861
                     /locus_tag="PLF66_18220"
                     /inference="COORDINATES: protein motif:HMM:NF019224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1554 domain-containing protein"
                     /protein_id="HMZ66032.1"
                     /translation="MGNIFIKLISILWLSVFFFECNPDKKDLTLRFIQPLSTNSNSQI
                     FLSNLTTSTGTLSPAFSQSITSYTVTVGSDITSISVTPTVNLSSVSITVNGTTVSSGV
                     ASNPISLNTGANTITIVVSQNNGLSTTYTLTVTRSPIVLTSLSGITTSFGTLSPVFSS
                     TTTSYSMTLANAVSSFTLTPTATDSSANITVNGSSVLSGNPSSPIALSVGSNTITIVV
                     SSGSSTTTYTINVIRLNSSVYRIFITAATYNGNLGGVTGADAKCNADVNRPNDGSTYR
                     ALVVDNSSLARRACSNPDCTNSAENLNWTFKPNTAYVRATDSAAILTTNAGGIFTFGT
                     LTNSFGTGATVSYWTGLRNNWQSSSNDCSDWTNSTGGIDGRTGDSTATNSNAIRNNTP
                     ACNTFWYLVCVEQ"
     gene            13215..14150
                     /locus_tag="PLF66_18225"
     CDS             13215..14150
                     /locus_tag="PLF66_18225"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HMZ66033.1"
                     /translation="MLNDEVKKYIDESVLCWLATSDKRNEPNVSPKEIFTYQDDLTIL
                     IANIASPKSIQNILENPIVCVSFINIFVQKGFKIKGDARIIERSSDRFEGAEKSLKRL
                     FTEEFPIQSIIEISVKKIDRIQAPSYFLFPNRTEKIQIENAMRTYKVQPLEKKKPSKD
                     IEIIEYNPSYKEFIKSLNYEWLEKYFRVEEGDILSLSNPQEYIIDKGGMIYFAKLNGM
                     IVGTVSLLKKSDSIYELGKMAVTQNAQGFGIGRMLLEYCLDIAKKKNIKTLVLYSNTS
                     LKAAIHLYEEYGFTEVELEKGLYERANIKMKKELF"
     gene            14332..14874
                     /locus_tag="PLF66_18230"
     CDS             14332..14874
                     /locus_tag="PLF66_18230"
                     /inference="COORDINATES: protein motif:HMM:NF016144.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HMZ66034.1"
                     /translation="MSLGLIKEYNEDWPNWFNQIKGILEESLWGLYLSIEHVGSTSIP
                     GMNAKPIIDIDIIIWRENFNRVLSHLTSLGYIHKGDLGITGREAFDLIQDKKEILPPH
                     HLYVCYEGEYELKKHLAFRDYLRKNKLAKEKLIKLKVELERTCKTKEEYIDKKDSLVK
                     ELTVLALKEYEEREYKKKSF"
     gene            15119..16189
                     /gene="rsgA"
                     /locus_tag="PLF66_18235"
     CDS             15119..16189
                     /gene="rsgA"
                     /locus_tag="PLF66_18235"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HMZ66035.1"
                     /translation="MNLLDLGLEKRFQEESAKYENLYLARVAEQFGDLYRVISEKGEL
                     QAKVSGKFSYDTSTNTDYPVVGDWVMIDRMEDTTGDAIIHHILTRKTVFKRKAAGTAN
                     QSQIVASNIDTIFICMALNHDYNLRRLERYLSIAWDSGALPVIVLTKSDLSKDLETQL
                     LEVESSAIGVSIIVTSNTISDGYSEIYKYLQKGKTIAVIGSSGVGKSTMINHLIGKEL
                     LATKEIRKDEKGRHTTSSRQLILLEKGGVVIDTPGMRELHLENADLSKSFEDIEEFAK
                     MCKFSDCTHTSEPGCQVLKEVENGNISQKRLENYKKLQTEISYESLNSRQVEEAKIQR
                     MFGGKKAMKQAMDYAKNKRNIK"
     gene            16285..17487
                     /locus_tag="PLF66_18240"
     CDS             16285..17487
                     /locus_tag="PLF66_18240"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HMZ66036.1"
                     /translation="MRKAVIVDGVRTPFGRYKGALKSIRPDDLASIVIQNLVERTKLD
                     RNSIDDVVLGCVNQAGEDNRNVARMSLLLAGLPEQIPGVTINRLCGSGLESINYAAMQ
                     IESGRADVVIAGGVESMTRSPLIMMKPEIGFSRGNHTLADSTLGWRFINPRLHQLYPI
                     VSLGESAEIIADKFHISREAQDIFSHESHEKAARAEEKGLFDKEIIPVYELNSEKILL
                     AKDEHVRRETTVEKLAELPPVFKKNGTVTAGNSSGINDGAAAILVMEESYALERGYTP
                     LAEVISSAVSGVHPSLLGTGPIPASLKALQRAGLNKDTIDLWEISEAFASMVLTTIKE
                     LNIEKEKVNVNGGGISLGHPLGASGARMALTLSHELIRRNLNYGMVTMCIGVGQGIAT
                     ILKRYERI"
     gene            18233..>21077
                     /locus_tag="PLF66_18245"
     CDS             18233..>21077
                     /locus_tag="PLF66_18245"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HMZ66037.1"
                     /translation="MKGVLDLQEWDFSKDGFLSLDGEWEFYWKELLSPETNPAVDANK
                     NLPKEIQYIPVPHVWNDFRVNGESIGKSGFGSYRLKIILPKQKDESSESQPSNISLAL
                     SNIASAHRIYVNGVLVGEKGKVGTSEEDSISFMQNYTIDLSRKLNQNKTNSDLNSVPF
                     PNELNIIIHVSNFNFTRGGIWLPLKIGTIDSIQKLENRAFVLDLIVFSSLLIMGLYHL
                     GLYFNRKKDKSPLYFGIFCILISLRTVSINQLMILDALPFVPYLIILKIEYFSFYFGT
                     FAFAKFSQILFPEEFSKKWLRFITIILIPCSLAVILFPVSVYLNLLTLVQLITLIAIV
                     YFVKVLILSSLKNKPGAKLSLFSFLIFALSVMNDILRSMGILYTPLLASYGFLSFVVF
                     QAIILSHRFAFGFEMAEKLGLELSQKSDRLEETAIELKELTQNLEAKVLERTKRLEET
                     QKEIQDLNEFTQKVNSLSNLDSIFTEISRYVYENYGILAVWLTLPDENKKFLYTYKIY
                     SYEKLPDNKYNYLKTKNVLMLDKDGGMVYKTFLRKKPLYLNKFPKFEFDIDRELVDKL
                     SLQSILLVPLVRKNESVGVLTFSNLNKEMKLSKNEIRKISNLCSQIAGAIDTNHLLEK
                     VEKAKRQTLELNQLIKVLNEKSNLIEIMEMLSFYINGKYKFPYYILSILDSQTNMLKY
                     AHAFLPTGLGEKEIEFIKSFNLPIDFNNKESIHSQALAEQKPLFLPDSSSGIKSNSGK
                     YILSFLKQKSSVTLPIILQKKPIAILDLFSQESVSLIQEDLVELSIFAEQLAGIINGS
                     LLFKEVLTEKEKVESARLEIEKLNEITRKINAESDLVNILEFIFSHLQEEYSIDGTIV
                     LLKDEQEENLRSFHTSAPIVNPEMIDYSRKLVIPLTDKEGGILYKAFSKNRTLYLAKV
                     SPNRADTELIKAMIETLKFTSFLAVPLQIKNESF"
BASE COUNT         7433 a         3111 c         3748 g         6785 t
ORIGIN      
        1 tcgtgcactt cgtggtttta aaaaagctaa tagtaaaaga tattctacca cgaaggacac
       61 gaaggaacac gaagttgggt tagtagcaaa aaaccaaaac ccttcgcgct cttcgtgccc
      121 ttcgtggttc aaaaaaagct gatagtaaaa gatattctac cacgaagcac acgaaggaac
      181 acgaagttgg gtgggaagta aaaacaaaac ccttcgtgct cttcgtgccc ttcgtggttt
      241 aattctttta tttcttttta agaaattgga ttttgttatc accaaagatg ccttaaactt
      301 agtggtaaag actggctaaa cttcccctat gtttatctcc ttagatatct ttacccatag
      361 tttaaggggt tttaagagaa gttgagtcta acgttattag ctagagtaga gaatgcgaga
      421 aaaacattgt attcatttcg ataaattttt tctcttctct tttgttttcc tttaaaaaag
      481 caatttactg tagtgttaat atgttctgga attgctgtct tttttaaaaa actctcatag
      541 gcagaatcca ctaaaagcac aaaaaactat gagtgatttc cctttctacg tgtgatacct
      601 attttttatt tctcactttt atgagaggat ctatataatc gctacaaata ttttgtagaa
      661 tcaatttaca aatcggagaa tcaaaatgaa aagaatttac acaaacctta ttgtgatagg
      721 ttttcttctc aacacaagtt gtatgagtat tatcaaaaaa gaagaggtag ggattttatc
      781 tagcccagga actgctaagg tcgtagacac tggaaaagta tatgttggga tcaatgaaga
      841 acttgtcctc tattctactc gctgggaaaa tcatgtagaa aaagtggacg ttatcacgca
      901 agatgattta catattgatg ttgttgcctc tattatcatg cgccctgtga aagaaaaaat
      961 tctagatgtt catttgaata ttggaaaaga ttattaccaa agaattgtaa aagcagaatt
     1021 tagaacagcg attcgaaacg tgatgacagg atatcaaatg atcatgattt ctaagaaatc
     1081 acaaacgatt gaggatgaaa taaaggactt agttgcaaga aaattatctg ccaagaattt
     1141 agaagtatca gatgtaaata ttgacgatat caatttaagt cctaagattt tacaagcggt
     1201 agaagataaa attgcaagac agcaagaatt ggaaactatg caatatgata tgaagattgc
     1261 acagcgaaat gaagagattg caaagatgaa agcaaagacc gaatcagaag tggaaattat
     1321 tagagcagag aaagaagatc aaattgctaa aataaaagtg aaaaccgaag ccgagtctga
     1381 tattatcaga gcacaagcta gagcaaaggc tcaaaaatta gtcaatgctc atttaacgcc
     1441 aaattattta aaattaaaag caattgaaag tcactataaa gcctttgaaa gtccaaactc
     1501 aaaaattatc tttataccgg ttggcaaaga tggattacct gcttatgtaa atatcaatga
     1561 taagatgttt ccaaatgtag cagaagatac taaatcaaaa aagacaaaca atacaataga
     1621 agagtaattt acgttagttg attttttatt tgcaaatatt tagaattaag ctagttttgc
     1681 atcaaggtat tctttgaacc aagatgcaga acatttatag attttcaaaa tctatctccg
     1741 atgaggagaa gataaaatca cccactgaat atttaagtcg agaagaagaa tttaaaaatt
     1801 tcgaatccga aatccgatct caaatccaaa atctcaaacc aattgagagg atttcttatt
     1861 taaataaaat ttcacaggag tttggtcgta gaattgcctt gccatgtcca gatgtattgg
     1921 tagcaatatg tccttattgt tctcgaaaga tatgggcaag gattcgaaat ggtttgttta
     1981 ctctttgtgc ttctttttgg attttagagt ctgatgatgg aaaatgggta acagaagatt
     2041 ctaaatgtca acatttcttt ctcttagatg gagccttgca tttaaattca aaaccgattc
     2101 ctgaaaaatt gactgcctat ggttttgttc gaaataaaat ctatatggga gcagaagttc
     2161 cctttattaa gcctcgtatt ttaaaaaaag agggggtaat tgctgttatc cattcaattg
     2221 cgattggaaa tggatataca ggatatccaa taacgtattt tacaaaggag aaaatttctc
     2281 agagtgagtt tgctatggga tgggcacgag aagaatacag cgcaaaagat gaacttgtat
     2341 ccaatgcaac ctttacaggc gttcgttttg aaccacagga ttatgattta gaaaaatgga
     2401 ttcagcttga taaaataaaa tggataatta gtcaaaagga ggatttttgt ttgaacaatc
     2461 cgaaagactt ttttccctat cttaatttag aaggtagaaa aaatccttat accattcaaa
     2521 acggaagaat actggatcta ccaaattcca ctcagataga gggaagtgca aatctagaaa
     2581 catttatgcc agggcagtag attaaaagaa agattgatct agtaagtcag tccctaattt
     2641 agatgtagat caaaaaggtt tttaaacagt ttatgacaat catgtttttg tttgaagttg
     2701 tctattttgt aatgaaaaaa gatagtattg aaagggaagg tcttatttta gtaaatagac
     2761 aaagttctga ttctaataga aaaatgatgc atcatgcgat tgtagtagca aagtgtcttg
     2821 caaaaatagc tctttagtaa attgaatcta tacttactat ctctgattat ttgaatgaaa
     2881 tggaatccgt tcgtaaaaat tgaaaaccat tttggttgga tctaaaaatt aggtgtagac
     2941 ggatccacta ttctagaatc agatacataa ttgttctatg attcaaatta aaaatttaaa
     3001 aaaatcctat tcttttggaa accagaaatt agaaattcta aaaggaatta acatcgattt
     3061 gccgcaagga aagttaatta cactcatggg tccttccggt tctggtaaat ctacttttat
     3121 gcatatacta tccggaattg atcgacctga ttcggggaat gtatttataa atggagagga
     3181 tatttcttcc ttttctgaaa aggatatcac tcagtttcgt cgaaaaaaaa ttggaatcat
     3241 ctttcaattt tttaatttga tgccttatct atccagtgtg gaaaatgtgt gtttgcctct
     3301 ctatctagct ggaattggaa aaaaagaagc ttatccaaaa gcagaaaaag ctttaagtct
     3361 tgttggtctt aagaatagat ttacacataa accgtatgag ctttctggtg gagagcaaca
     3421 aagagttgca attgcccgtt ctattgtgaa cagtcctgaa atcttatttg ctgatgaacc
     3481 aactgggaat ttagacactg aaaattcaga tcatatcatg gagcttttga ttcaattgca
     3541 gaaggatcat ggatttacaa ttgtaatggt tacgcataac gtggagattg ggaatagggg
     3601 agatattaag ctttctatga aggatggaat tttagtttga attctaggct aatatattta
     3661 tttttaatag agtattttag aactcataaa gtaaaaacct tcttctctct tgttggaatt
     3721 gttttgagtg ttgctttatt tgtgactaca acgttcaatg gagagagagc agaaaagtcc
     3781 ttgatcgatt tttcaatggg ttatctagga gatagatacc aagcgagaat taaaaatcta
     3841 aatgataaaa ttgggatcac tgattctgat atagagaatt tattctattc ggaagaactt
     3901 cgttttatag ataaaatcat tccaagaatc cacaagactg taaaattaaa aacaaatcaa
     3961 gcaaccttaa acgttattta ccaaggaatt gatataatca aggaaagaga agatttaacg
     4021 atcacatcca tctgcagaaa ttgtaataga gaatccaaaa catttatcag ttctgctcta
     4081 tttgataaaa ttgggactga tagttttagt tttacgattg agagtgtaga atacaaagtt
     4141 actaacgcag ctgtcgttga ttcagaggga ggacttttta tactagaaga tgttgaagat
     4201 gtaaaagaaa gactttctca gacctattat gattcgattc tactcagtat aagcaataca
     4261 acaaatgaaa atctttcaat cctaaaaaca agtttatcta gttacaataa aaattttcaa
     4321 ttagaaacga gcgaggaagt taaaaataga gcatctaacg ttttaaaatc ttttcattta
     4381 aatttgatta ttatatctat gatatccgtt ctaatcgcat tttttatggt atcgaataca
     4441 atgacaggca tatttttgaa tagaaaaaga gagttgggta tattgcgatg tttgggaagc
     4501 agccaacttg aaaatctgtt tttgttttta tctcaatctg ttgtgcttgg agtgattgga
     4561 actgttttag gaattttact tggaaccttc ttatctaaac atagtttttt tagtggggaa
     4621 tccacactga cagacataaa tcaatcaata tcctatacaa aaattccatt taaaattatt
     4681 ttaatcagtt cgatgattgg aatttttggt tctgtcttat cgggtctatt tccagcactc
     4741 agatcatttc ggttggctcc aattaccatt gttcgggaag atccaaatca gacatccgtc
     4801 agaaatttca aaactttatt ctatgtaggt attcttttta tcggagtcgc ttatttactg
     4861 acacagattc gatcctcttc caatattccg atattcggat tgattgcaat tggtatgata
     4921 atcttagggc aaacgatgtg ttttccccaa ctcatgaaaa tatttttgag ccttgcgaat
     4981 caagttttcc aatcactcga tcattccttt atcgaaacta ggattggatt tgaagagatt
     5041 gttcataata cctataaaaa tactttaaca tccgcaacat taatgcttgg agtatcgctt
     5101 atcgtatcac tttctgtttt aacacttagt tatgaaagat caattatgaa ttggacagaa
     5161 cgtgagttcc catttgattt ttccataatc aatcaaaaag atttggagga aggagaagat
     5221 ttaggagttc caatctctct ttcttctaaa attgcaaagt tggaaaatgt ggaggaggtg
     5281 gatgtttttg ttttaaatac caaagcagaa gtgggagata aaatttttac aatccatgcc
     5341 tatgatatgg aacttacaaa gattcgagag ttaaaaaaag gaattttcat tcactcagat
     5401 atcaatctaa aagaagaaat ccttgtttcc tccaatatgg cttatttaaa ccatctaaag
     5461 aagggagatg agatcttatt aaataccaga aaggggaaaa agagtctgcg gatcgcaggg
     5521 atgcgagaac attttttttc tgagaatggg acgattctta tggactatcg tttgtatgat
     5581 gagctatttg gaattgataa gtttagttcg attcgagtta atttcaaaga taaaggaaat
     5641 ttagaggatg atttggagag gttaaaacaa gagattgcac ctttccctaa tttgaaacct
     5701 atgaatggac aagaattaaa agacatctat ctaaacggac tcaaacgagt ctttaaagtt
     5761 ttagaatctc ttaaatatac tgccgccttg attgcgtttg tatctctgtt ttcgtctatc
     5821 ttatataacc taaatgataa acttcgtatc tttggtgtat ttcgttctat tggttctagc
     5881 cagatgcaat taaatcaaat tgtatttgcg gagaatctat ttttaacttg tttcggaatt
     5941 tgcatgggaa ttctgtcttc ccttctactg agtccaatta tacttcatgt aatcaataaa
     6001 acagcctttg gatggacact aactgttgaa ataccatatt ccttgctatt cgtttgtatt
     6061 ttgtctatac cgtttgtttc tttttttacg actctctatc cttatttgat tctaaaaaaa
     6121 ttgagtttac gagaaatcct ttcctatgaa taattcgaat agaaggattt taccacagat
     6181 gaacacagat ggatacggat gcggtctgtt taaaatcaac accgatggac accgataact
     6241 aaacgattag aaacgatggt tttatcggtg taagcgaagc gatcggtgtg aatcagtggt
     6301 aatctttgaa aatttttctg aactagatga ctttgagggc ttggtaactg attttccatg
     6361 catcgaatcc atgttatttt taagttagaa tctacgcata ttgagaggaa atttggttta
     6421 attaccgaaa atataaacaa cacctatgtc tgtttcaaat gttaaaattt tacaagatat
     6481 tgccaatcaa atcaaagaag aaaatttgag aggaagagag cggattccaa cctccgaaac
     6541 ttttattaaa agaatgatga ctttgcattc aaagagtgca gaggatataa agtttttctt
     6601 ggagcagttg aaggaattgc attatatttt tgtgattact gtggttgcct ctgatccaac
     6661 tctttttgtc caaggtgtgg atgcgtatgt ttatgcagaa ctttctatct taaatgagct
     6721 gaaacattat accgagcaaa gacttactca agtctatgaa agcacttatt ataaaagaaa
     6781 atctggattt cagattattc gagaattgct tccaaaaata aaagaattta acaatacaca
     6841 attaggtaga aatctaaatg aagcgatcat gttagatgag tatagtcgct taatcaccac
     6901 gagtcctttt gaatatacgg attcatggag aaaggagaaa ctatataaac tttatagaga
     6961 agaagagccc aattatgaag tagaatatga agtggagggt aatagtcctt atccgcagag
     7021 cacagatcgt agagtgcaag attcaggtaa tagtaaatgg gctaaagcag taaatcagtt
     7081 ctcgattcga ttcttaattc gaattcattt tagaaaatat gaatttgatg tagttaaaag
     7141 acttgtccta acgtctaaaa tttcaatgtt agatgatttg atttatattc gaaatacatt
     7201 aaaagattta gagaagcaat tagataaaga tacaatcttg aaatatcatc tagataaaat
     7261 aatcgagcta aggcgaataa cgcaagctaa aattaataat ttacgaaaga ttcagcaaag
     7321 tgcagcaggt tcagtatgag tagaatatta atagtcgatg atgatattgt tatattagaa
     7381 acattgaagg atgtattgac agctgctaaa tacaatgtaa ttgcttcctc taatcccgtc
     7441 gaagcgctaa aaatttacaa agaaaatcca actcttgtgg ttattaccga tcttattatg
     7501 aatgaaatgt ctggaagaga tctaatgata aaactatttg agactggtca taaacctcta
     7561 gtcattattc ttactcgtat gaatgatagt aagacaatca ttgatctatt taaaagtgga
     7621 gcccatgatt atacaatcaa accttttgcg gtaagtgaaa tagtcgcaaa agtaaacaaa
     7681 gcttttgagt tggcagaact tcgaatcgta aatgaaaacc ttaaaaggga aagagaaatt
     7741 cgttttgaaa atcaattcaa atggaatatt tttcgagaca attcaaaaaa taccaagtct
     7801 gatattcatt taattgatgg aattaagact agctttttgc aaggatcagg aattggtgct
     7861 cttgttccaa ttattgatat gattaagcaa tcctcgaaag aagaagggga agtctttcta
     7921 gttaagaaaa agcttttaaa gatgcttttt tcgaatgcag agtttttaaa tcgattaatc
     7981 gaaatcatag ctgatataga tcatgtcagc aatatggaaa ttccaaaaga gaaaattagt
     8041 attcatattc ttcatgagtt tattgagact gtaatcaatg acctagataa atatgccgcc
     8101 ttgaggaatc actcagtagt attaaccaaa aatgattatt caaacgttag tagatctatt
     8161 cttttaaaca aagagtattt cagaaaagcc ttgcaggaac tacttttgaa tgcgtttaaa
     8221 ttttctgaag aaggtagtaa aatctttatt ttatttgaag taataaaaga acaactattc
     8281 atttcaattt taaatcatcc tgattcaaat ggaaaagagt tgaatgggat tttacctgaa
     8341 tttcaagcac ttgtatttga accatttttt cgtatatcta gatatgttca tgaagagata
     8401 ccaactcttg attttggttt aggtctttgt tttgtggatc gaatcatacg caatcacaat
     8461 ggtaagatca aagtagggaa tataaagaac cacaccaatc caaatggaat tctaattaac
     8521 tttacaatcg agattccttt tgcaaatgat tgattctcta aaacgaatct tttttataac
     8581 ttttattttt cttttttttt atcaatgcac cctccatcat tatgagatgg gtagaaaacc
     8641 aattcgaatt gtagattcat tagaattgcg agaaaaaaga aaaatgagtt tagatagaat
     8701 tccaaatcca atagagagta aaaaaaaaga caattcaatt gcaagtagct ataattcgga
     8761 tggattcaga gattatataa aaaggggatg ctgcatagaa attgtagaag atgcaaaatt
     8821 aaaaatacgt tattcttcca acgtcaaatc agatctttta tacgctccaa ctgttttttt
     8881 atggatgttt tctttgggtc tcattcctgt ttttgaaaaa gtggatgctg agataaaaat
     8941 tgaagtttta gattcaaatg agaatattct cagggaatat tattacaata ttgaagaaca
     9001 tgcttataat tcttggctta cgattccagt tagtttaatt ctcatctcaa atgattcatt
     9061 tgcccatagt tttattcata tgtttgatta tcctcaaaga tttattgcaa ataaattcga
     9121 aactgacttt caagaggatt tagctcaagg aaaaatttct gaaaatataa acatactcaa
     9181 tcaatcgaaa gacatacaaa ataaatctag aatcgctatt ttaccaattc ggcatcagtt
     9241 ttcgaaggat caaatgatag caaatgcaat tcgggataaa gttgaaacta ttcttgtaaa
     9301 taaaaacttc atagttgtag aaagagtaaa gttagacgaa atactaaaag aagtaaaact
     9361 ttctcaaaca ggtctaacaa catctgagca ggcacagata ggaagtatgt taaatgcatc
     9421 taatttaatt gtaggtgagt tgctagacat tgatcgaaat gattctacat tggaattctc
     9481 tcttcgtaat gttgatatgg aaacagggaa gattctttgg aagtatgaat tttcgatcga
     9541 tgagaaaaat tcaacccaat ccttacaaga agcaatgaaa tccctagatt caaagatcaa
     9601 accaatgaag tgaaattaaa actctttgct ataaccaaaa ctatagatag aactattttt
     9661 atttagaata gaattgggtt ccactttaga ataaataaaa aatccatttt gtttttcata
     9721 cataggattt tttaagctgg aaaaatcatc agaccataaa agtgcatcta gaatattaaa
     9781 aacgtatatt aagattaaaa aagtagaata aacggcaact tctgctgatg caatatccga
     9841 tttctttttt gcatcttctt tttcgttttg cataaatgta taagcgagtg tattatttct
     9901 taggaatgaa ttggagacca aatagtttgc agttctgtat ctagtttttt cttggtgata
     9961 atcatgattg gctcttagat ctagactcag taatccaaaa aaactaataa aaaagagact
    10021 tccttttact ttataaccat tcataaattg accaagacct ggaactaaaa aggaaaggta
    10081 aggagaaact tcgctaggtt ttattcctag atctaattct ttagtgtctt cttttgtatc
    10141 agaataggct gggctcggat ttagagagga gcgatctttg tcaggattta ataccatctc
    10201 tcttatttta agtgttagat tttctactgc taagtgcatt ccatctaagg aagatgtagt
    10261 ctcatgctca gccacttcct ctacgccttt ttcaatatcg attagatatg catttatatg
    10321 atagcttcgt cctagtttaa aaattttgcc tgtcataatt ttatttgcac ctaagagtct
    10381 tcctagacga atgttgcaat ctgtggtatc acataaacca agttgaatat tcttttgctc
    10441 ttctagaatt ttatttagat tttctctgtt tagaattgta aaagtattta aattgatcat
    10501 ctcagtgcgg ataatgtttg ttatatcctt agcttcttgc ggtgttactc cattgccatt
    10561 tttgaaatct aaaatagcaa ccctatactt tgtgcgcgcc tcgttttctg atatggagtc
    10621 ctctgataag agtggaacta taaaaaaaga gataagaaat agaaaagaga gaaatgattt
    10681 catagatatt aaaatcaatg tatatgttaa attcagcaag gattttaaat ttgattttca
    10741 aatatttttc tgaattgata gaaagatacg aaggatacac taaggagtct aacgatagtg
    10801 aattagacac tctaattcta ggaaaaacat aaatcatttt cctagtaaaa attaaattta
    10861 gctacaagtt tggattttat ttatctaatt caaattcaat tataattgca taacaaaaga
    10921 aataatagag tttagatgag ccaaagattt aaatgaggtt tgctcttatt gtcattaaaa
    10981 actaataatg ggttgctagt aaaattagtt tttcctatta ttatcaaatt gatttttaca
    11041 aaaatcaaaa cgattcgtat aattgtcttt tctatttaaa aaaaacacta gtagaagaaa
    11101 ttgaccaata gctagaaaaa tgcactggag caatttagag aatatgaaaa aattatttct
    11161 tatctttatt tttatgccaa ttctattaga gtctacggat agaaagaatt caactacaaa
    11221 ctggaccgaa tatttaggaa taatgaattg gaatgaagca aatcaaaaat gtaaaaaatt
    11281 gggaatgcgt ttaccaagtc gaagtgattg gctagaacca tataactctg accaaagaaa
    11341 gagttggaga aaaccaaatg gaagttggtt ttctgtgtat tggacttctg aggaagtatc
    11401 tggtgctggt gcttacgttt ttagtctaga ggatggagat cctgatcgtt atgcgaaaga
    11461 tatgaaagtt catgttcgat gcattcgcct aaaataatct gagacacaag ttaaaaatgt
    11521 ggagtctttt atttaaatgc aagtgaaaat acgggtaaaa aatgtaaatt tttataagaa
    11581 gcagtttaaa aataaataat ttactgaata ttgcaacgtt tttaattgtc ttatttatta
    11641 ggtatgggaa atatatttat caaactgatt tcaattcttt ggttgtctgt ttttttcttt
    11701 gagtgcaatc ctgataaaaa agatttaact ttaagattca ttcaaccact ctcaacaaat
    11761 tcaaattcgc aaatatttct ttctaatttg acaacgagta caggaacctt gtccccagcg
    11821 ttttcacaat ctattacatc ttataccgtc acggttggat cggatattac ttcaatttcg
    11881 gttacaccaa ctgtcaattt gtcctctgtg agtataacag taaatggaac aacagttagt
    11941 tcgggagtcg catctaatcc gatttcctta aataccggtg ctaatacaat tacgattgtg
    12001 gtaagtcaaa acaatggtct atctactact tacacactca cagtaactag atcgcctatc
    12061 gttttaacca gtctgagtgg gataacaaca agctttggaa ctttgagtcc cgtatttagt
    12121 tctactacaa cttcttatag tatgacgcta gcaaatgcag tttcgtcctt taccttgaca
    12181 ccaacggcta cagattcgag tgcaaatatt acagtgaatg gctctagcgt gctgtctgga
    12241 aatccttcta gtcctatcgc tctgagtgtt ggttctaaca caattacgat tgttgtcagc
    12301 tcaggaagtt ctactacaac gtatacaatc aatgtaattc gtttgaattc atctgtttat
    12361 agaattttta taacagcggc aacttataat ggaaacttag gtggagtaac aggagcagat
    12421 gcaaagtgta atgctgacgt taaccgcccg aacgatggaa gcacttatag agcacttgtt
    12481 gtagataatt ccagcctagc aagaagggct tgttcaaatc ctgactgcac aaattcggct
    12541 gaaaatttaa attggacttt taaaccgaac acagcttatg taagagcaac agattctgct
    12601 gctattctta caacgaatgc aggtggtata tttacatttg gcactttaac gaattcattt
    12661 ggaaccggag cgacagtttc ttattggaca ggtcttcgaa ataactggca aagttcttcg
    12721 aatgattgtt ctgattggac gaattctacg gggggaattg atggaagaac aggagacagc
    12781 accgccacca attcaaatgc aatccgaaac aatactccag cctgcaatac attctggtat
    12841 ttagtctgcg tggagcaata gggaaagata gaaaggttac cactgattta cacagatgag
    12901 caccgataca attctatcga ctcataatcc taaatagttt tttaaagaag gatcagagaa
    12961 gggtaattaa aagtatggga atttgattta tatcgaaacc agctaatact aattatccgc
    13021 tgcaagtcta caaagatgtg aaatggttat tccagtgttt ctttttcaaa gagaattgcc
    13081 ctcaactcta gaatttttta tttacaattt cagaatccca aaaggatact aaaaatggcg
    13141 acttgctttg tgtcgacaaa ttcaaagttt aaagagaata aaaattaaaa caataggaaa
    13201 tggagcttat aaatatgtta aatgatgaag ttaaaaaata catagatgaa agtgttttat
    13261 gctggttggc aacatcagat aaaaggaatg agcctaacgt ttctccaaaa gagattttta
    13321 cctatcaaga cgatctaaca attctaattg ccaatattgc ctctcctaaa agtattcaaa
    13381 atattttaga aaatccgata gtatgcgtta gttttattaa tatttttgtt cagaaaggtt
    13441 ttaaaataaa aggggatgct cggatcattg aacgttcaag cgatcgattt gaaggagcag
    13501 aaaaaagttt aaaaagatta ttcactgaag agtttcctat tcaatcaatt attgagataa
    13561 gtgttaaaaa aattgataga atccaggcgc ctagttattt cttatttccg aatcgaacgg
    13621 agaagatcca gattgagaat gctatgcgaa cctataaagt ccagccatta gagaaaaaaa
    13681 agccttccaa ggatattgaa atcattgaat acaatccaag ttacaaggag tttataaaat
    13741 cactcaacta cgaatggtta gaaaaatatt ttcgagttga agagggtgat atactctccc
    13801 tttccaaccc tcaagaatat attattgata aaggcggaat gatttatttt gctaaattaa
    13861 atggaatgat agttggcact gtttccctcc taaaaaaatc agattctatt tatgagcttg
    13921 gtaaaatggc tgttacacaa aacgctcaag gatttggaat tggaagaatg cttttggaat
    13981 actgcttaga tattgctaag aagaaaaata ttaaaacact tgttttatat tcaaatacct
    14041 cattaaaggc ggctattcat ctttatgaag agtatggatt tacggaagtt gaattagaaa
    14101 aaggtctata tgaaagagcg aatataaaaa tgaaaaaaga acttttttag ttctctccgg
    14161 ttgcagttag attcaattca aatttttctc caaatgaatc taagcataag aaaaatttct
    14221 ttataaaaag gaatgaagat tagtagtata cattctaatc tttcattctt gtgaaccgat
    14281 agttcccttt gaaaatggaa gtaagaaaaa gaagattaag atatttttaa aatgagctta
    14341 ggattaatta aagaatacaa tgaggactgg ccaaattggt ttaatcagat taaaggtatt
    14401 ttagaagaat ctctttgggg tttatattta tctatagaac atgttggaag cacatctatt
    14461 ccgggaatga atgcaaaacc aatcattgat atagacatta tcatttggag agaaaatttc
    14521 aacagggttt tgagtcatct cacttcgctt ggatacatac ataaagggga tctagggatt
    14581 acgggaaggg aagcatttga tttgatacaa gacaaaaagg aaattttacc tccccatcat
    14641 ctatatgtat gctatgaagg agaatatgag ttaaaaaaac atttagcatt tcgtgattat
    14701 ttaagaaaga ataaacttgc caaagaaaaa ctaataaaat taaaagtaga attagaaagg
    14761 acttgcaaaa caaaagaaga atacatagat aaaaaagatt ctctagtcaa ggaactaact
    14821 gttctagcgc taaaagagta tgaagaaagg gagtataaaa agaaaagttt ttaagtgtat
    14881 tgagcttact atttcttttc tatcgctctg ttcgatctga ttcacatttt gaaataagat
    14941 agaaagattt gaatcaatac aactcaatca ttactctttc taaacaatca ttgagttagg
    15001 tgaattcata atttttttta acagtataaa agaggagatt ttattttact tgttgttttg
    15061 aagtatttgt atatcgccaa attaatatga gtattaaata ttgaatttag aagggaaaat
    15121 gaatttacta gatcttggat tagagaagcg ctttcaagaa gaatctgcaa agtatgaaaa
    15181 tttatatctt gctagggttg ctgagcaatt tggagatttg tatagagtta tttcagagaa
    15241 aggagaattg caggcaaaag tttccggtaa attttcttac gatacttcga ctaatactga
    15301 ttatccggta gtaggtgatt gggttatgat tgatcgaatg gaggatacta ctggagatgc
    15361 aatcattcat cacatactca ctcgaaaaac agtattcaaa agaaaggcag caggaacggc
    15421 aaatcaatct cagattgttg cttctaacat tgatactatt tttatttgca tggctttaaa
    15481 ccatgattat aatttgcgaa gattagaacg ttatctctct attgcttggg atagcggagc
    15541 attgccagtc attgttttga ctaaatcaga tctaagtaaa gatctagaaa cacaactttt
    15601 agaagtagaa tcatcagcga tcggagtttc aatcatcgtt acttctaata caatatccga
    15661 tggatattca gaaatttaca aatatttaca aaaaggtaag acgattgcag tcattggttc
    15721 ttcgggcgtt ggaaaatcta caatgatcaa tcatttgatt ggaaaagaat tacttgccac
    15781 aaaagagatt cgaaaggatg agaagggtag gcatacaacg agtagccgcc agctcatttt
    15841 attggaaaag ggtggagtag tgattgatac tcctggaatg cgagaacttc atcttgaaaa
    15901 tgctgatctt tcaaaatctt ttgaggatat tgaagagttt gcaaagatgt gtaagttttc
    15961 cgattgcacc cacacttctg aacctggatg ccaagtttta aaagaagtag aaaatggaaa
    16021 catctctcaa aagagactgg aaaattataa gaaattacag acagaaatta gctatgaaag
    16081 tttaaattca cgtcaagtgg aagaagcaaa gattcaaaga atgtttggcg gaaagaaagc
    16141 aatgaaacaa gcaatggact atgcaaaaaa taagagaaat ataaagtagc attgaaacca
    16201 aataaatcaa attatttgtt atgaatctag attgattcag tttctcgaaa tgctactgaa
    16261 tcaaaaataa aaaagaagta aagtatgaga aaagcagtaa ttgttgatgg cgtgagaact
    16321 ccctttggaa gatataaagg tgcgctgaag tcgattcgtc cggatgatct agcgtctatc
    16381 gtaattcaaa atttagtaga gagaacaaaa ttagacagaa atagtattga tgatgtagta
    16441 ttaggatgtg tgaatcaagc aggggaagac aaccgaaacg tagctagaat gtcactcctt
    16501 cttgcaggtc tcccggaaca aattccaggt gtcacaatca atcgactttg tggatctggt
    16561 cttgagtcga tcaattatgc ggcgatgcaa attgaatcag gtagggctga tgtggtaatt
    16621 gcaggcggag tggagagtat gactagatct cctctcatta tgatgaagcc tgaaattggt
    16681 tttagtcgtg gaaatcatac gttagctgac tcgacattag gttggagatt tatcaatcct
    16741 agacttcacc aactttatcc gattgtaagt cttggtgaat ccgcagaaat cattgcagat
    16801 aaatttcata tttctagaga agcgcaggat atattttccc atgagagtca tgaaaaagca
    16861 gctcgggcag aagagaaggg actctttgac aaagaaatca ttccagttta tgaattaaat
    16921 tcagaaaaaa tacttcttgc aaaagatgaa cacgttagaa gagaaactac tgtcgaaaaa
    16981 ttagcagaac ttcctcctgt ttttaaaaaa aatggaacag ttactgcggg aaattcatcc
    17041 ggaatcaatg atggagcggc agctatctta gttatggaag aaagctatgc tttagagagg
    17101 ggctacactc cattagctga agtgataagt tctgctgtat ctggagtgca tccttcttta
    17161 ctcggaacag gaccaattcc tgcttcttta aaagctctac aaagagcagg actaaataaa
    17221 gatacaatcg atctctggga aatctcagaa gcatttgctt ctatggtttt aaccacaatc
    17281 aaagaattaa atatagagaa agagaaggtg aatgtgaatg gtggtggaat ttctctcgga
    17341 catccacttg gagctagtgg tgctcgtatg gcgctcactc tctcccatga gctaatacgg
    17401 cgtaacctca actatggaat ggttaccatg tgtattggtg tagggcaagg aattgcaacg
    17461 attctaaaaa gatatgaaag aatataaaat tcaacttatg ctatcactct tgaattcaaa
    17521 aaataattga gtttggttga atcaaatctt ctaatcagtc ttgcaactat cgttaataaa
    17581 aaatgacgat agactttgca aaagataggg tcagagatct ctcaactctt ctccatttta
    17641 tacactagag tcatgcaata ttttagaatc gattttataa ttttatctac attctctaaa
    17701 attttaaaaa ttattcttag cttgctaaat tctaaaattt atataaaatg aataaagtat
    17761 ttttttaaca gatcataagc ttaaaataaa taggattgat tctaaaggga tcattgtttc
    17821 taagcataaa ttaaaagtat ccaaggtgtt ttgaatgaat ttgaaataca aacaagcatt
    17881 ttttatcctt gaatttaact tgaaagagaa atgaatattt tgagctagaa tctataagaa
    17941 acgaaaaatt agaattataa atttattttg aatgggaaaa aatcctgcct ctatttcaaa
    18001 agaagtccct gcttctttag aaaaatttaa aactgataga aaaggttcat atctattgca
    18061 atgaaattat tttaagaaca atgacagata acgccaattt ttgcttgcta atatactaca
    18121 ggaacttagg cttcagtgaa tgttatgtcg agtaatactt cttgttttta tttttctatt
    18181 cttagttttt tcaaattgca atgacaccaa gaagaaagaa tcacccaaag tcgtgaaagg
    18241 ggtgctagat ttacaagaat gggatttttc aaaagatggt ttcctatcac tcgatgggga
    18301 atgggagttc tattggaaag aattgttaag cccagaaacg aatcctgcag ttgacgcaaa
    18361 taagaatctt ccaaaagaga tacaatacat tcccgttcca catgtgtgga atgattttag
    18421 ggtcaatgga gagtctatcg gtaaaagtgg ttttgggtcc tatcgtttaa aaataatcct
    18481 ccccaaacaa aaagatgaat ccagtgaatc gcagccttct aacatttctc ttgcattatc
    18541 caatatagct tccgcgcatc ggatttatgt gaatggagtg cttgttggag aaaaaggcaa
    18601 ggtaggaacg agtgaagaag attctatttc ttttatgcaa aattatacaa tagatctctc
    18661 cagaaaattg aatcaaaata aaacaaatag tgatttaaac tctgttccct ttccaaacga
    18721 attaaatata attattcatg tgtcaaattt caattttacc agaggcggaa tttggctacc
    18781 tttaaaaatc ggaactatcg attcaattca gaaattagag aatagagcat ttgttttaga
    18841 tttaattgta tttagttccc ttttaattat ggggctttat catttgggtt tatactttaa
    18901 tagaaaaaaa gataagtctc ctctttattt tggtatattt tgtattttaa tttctttgag
    18961 aacggtttcc atcaatcagc tgatgatttt ggatgcacta ccttttgtgc cgtatttaat
    19021 cattctaaaa attgaatatt ttagtttcta ctttgggact tttgcatttg ctaaatttag
    19081 ccaaattctt tttccagaag aattttctaa aaagtggtta cggttcatca cgattatcct
    19141 aattccttgt agcttagctg tgatcctatt ccctgtatct gtatatctca acttacttac
    19201 tttggttcaa ttgattactt tgattgccat tgtatatttt gtgaaagtat taatcctatc
    19261 ctctctcaaa aataaacctg gagcaaaatt atctctcttt agttttctaa tttttgcatt
    19321 atctgttatg aatgatatct taagaagtat gggcatttta tatactcctc ttttagcaag
    19381 ctatggtttt ttatcctttg tagtttttca ggctatcatt ctttcgcacc gatttgcttt
    19441 cggatttgag atggcagaaa aattgggatt agaactatcg caaaaatcag atcgtctaga
    19501 agaaacagca atagagttaa aagagcttac acaaaatcta gaagcaaaag tcttagaaag
    19561 aactaaaaga ctagaagaaa cacaaaaaga aattcaagat ctaaatgaat tcacacaaaa
    19621 agtaaattct ctttcgaatc tagactcaat atttactgaa atttcccgtt atgtttacga
    19681 aaattatggg atactagctg tttggcttac tcttccggat gagaataaaa aatttctata
    19741 tacatataaa atttacagtt atgaaaaact tccagataat aaatacaatt atctcaagac
    19801 taaaaatgtt ttgatgttgg ataaagatgg tggaatggtt tataaaacat ttttaagaaa
    19861 aaaacctctc tatctaaaca agtttccaaa gtttgaattt gacattgata gagaattggt
    19921 cgacaagctt tctctacaat ccattttact tgttccacta gttcgaaaga atgaaagtgt
    19981 tggagtttta acattttcta acttaaataa agaaatgaaa ctttcaaaaa atgaaattcg
    20041 taaaatttcc aatctttgct cccaaattgc tggcgctatt gataccaatc atttattaga
    20101 aaaagtagaa aaagcaaaac gtcagacgct tgagttaaat caactcatta aagttctaaa
    20161 tgaaaaatca aatctaattg aaattatgga aatgctttcg ttttatatta atggaaaata
    20221 caaatttccc tattatattc tttccatatt agattcacaa acaaatatgc ttaaatatgc
    20281 tcatgcattt ctaccaaccg gcttaggtga aaaagaaatt gagtttataa aaagttttaa
    20341 tttaccaata gattttaata acaaggaaag cattcactca caagctttag cagaacaaaa
    20401 accattattt ctccctgatt cttcctccgg tattaaatca aatagcggaa aatacatatt
    20461 atcctttctg aaacaaaaat cttctgtcac tctcccaatc attttacaaa aaaagccaat
    20521 tgcaatttta gatttattca gccaagagtc tgtatcttta atccaagagg atttagttga
    20581 gctttctatt ttcgcagagc aactagctgg aattatcaat gggtctttgc tatttaaaga
    20641 ggtattaacg gaaaaagaaa aggtcgaatc tgcaagactt gaaatcgaga agctaaatga
    20701 aattacccgc aagatcaatg ctgaatcaga tttagtcaat atcttagaat ttattttttc
    20761 ccatttgcaa gaggaatatt cgatcgatgg aaccattgtt ttattaaaag atgaacaaga
    20821 agaaaactta cgttcttttc atacgagtgc acctatcgta aatccagaaa tgattgatta
    20881 ttctagaaaa ttagtaattc ccttaactga taaagaaggg ggaattttat ataaagcttt
    20941 cagtaaaaat aggacccttt acttagcgaa agtcagtcca aatcgtgccg acacggaatt
    21001 aatcaaagca atgattgaaa cattaaagtt tacttcattt ttggctgttc cattgcaaat
    21061 caaaaatgaa tcctttg
//
LOCUS       DAOQOS010000095        20983 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_1226_length_20983_cov_5.670155, whole genome shotgun sequence.
ACCESSION   DAOQOS010000095 DAOQOS010000000
VERSION     DAOQOS010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 20983)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20983)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20983
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1226_length_20983_cov_5.670155"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..884
                     /locus_tag="PLF66_18250"
     CDS             <1..884
                     /locus_tag="PLF66_18250"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HMZ66038.1"
                     /translation="YTYYDLERWREEIENKKIQINQENTLKDKLLKEGDEISFTIPDF
                     IEPEIDSDYSVLYEDEYYFAIGKSGNLPVHPAGRYRKNNLTTLLESDSRLPKPFYLIH
                     RIDRETSGIVLFGKSKLAASKLGELFEKNKIQKTYLVYVEGVFPESLEAKGFLEKDEK
                     SKIRKKKKFTFEKTKDILWEVYTDFARVEVEGNISKIKAFPHTGKIHQIRATLHSLGY
                     PLLGDKIYGRDESYFLEFIETGKARENERIERQALHAFSITFPHPFLKKEISIECVEP
                     LDMQKIFSNSPYIKLTL"
     gene            complement(1130..1924)
                     /locus_tag="PLF66_18255"
     CDS             complement(1130..1924)
                     /locus_tag="PLF66_18255"
                     /inference="COORDINATES: protein motif:HMM:NF017532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HMZ66039.1"
                     /translation="MHQFKKEIFNGKILHKKDILSSYVLKDFVYLFLEKKLCTKDLQT
                     FEKKVSPEKFHQLFSEIKEELTKNLTIKKRIANLLKEFNYKIETTYIDNLRLRCVTSY
                     LHTIDNAKPAFAIHRDTWYANPETQINFWIPIFSVDEKDTFAFYPEYFDKPIQNDSNL
                     FHLENWNLSGGFQANPKFETQRIFPEAKVDLPQTSELKIPMQAGELLIFSASHLHGTR
                     PNQTNKTRFSIDFRVIDTSDLSGAPNIDNFSKGSILSQMWRLSDII"
     gene            2232..3335
                     /locus_tag="PLF66_18260"
                     /pseudo
     CDS             2232..3335
                     /locus_tag="PLF66_18260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010572665.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="catalase family protein"
     gene            3633..4115
                     /locus_tag="PLF66_18265"
     CDS             3633..4115
                     /locus_tag="PLF66_18265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020779233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HMZ66040.1"
                     /translation="MNEPLNHSVKDINGNLVNLNEFKGKTTLIVNVASKCGLTPQYKG
                     LQELYDKYKDKNFVILGFPSNDFLGQEPGTENDIKQFCELNYKVSFPMFSKIKVKKGD
                     GQADLYKYLTSKETNPGFEGEIEWNFQKFLVDKNGKTVKRYSPKTTPEEIAKEIENYL
                     "
     gene            4304..5452
                     /locus_tag="PLF66_18270"
     CDS             4304..5452
                     /locus_tag="PLF66_18270"
                     /inference="COORDINATES: protein motif:HMM:NF016196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 family protein"
                     /protein_id="HMZ66041.1"
                     /translation="MFKEELFIKKTPYRKKQIIAVSLLFLSGISLILLETKLILANQE
                     MIRKILINAFEGGFVGGLCDWFAVWKTYNAIENDSLIVADEIGQWVASDLLDKNTLKN
                     QINEVLDSAETNAEIYKLLDTYFDTKENTKKILENLWIKMEPSVIEYIVHYNFSLSEM
                     SLITNASSDQIIINTIKICVGDTLSKISEEQKFKEFIAKIVSQQNAMTKFVSIFINIP
                     SIIKMYGEKLKSGDDSFSENEQFLDEMVTIISMSADKYILSWQSLSTERKTVAVKALI
                     TQLRETSLDVAAKYIILHKNHIKASKTLRNYMPVREFFEFIEDKIDDQVPQFIGHKIS
                     ERLKSQDPKDFRVNIEWKTRNILENIRINGTILGFFLGLLIGVLKYSI"
     gene            5637..5870
                     /gene="acpP"
                     /locus_tag="PLF66_18275"
     CDS             5637..5870
                     /gene="acpP"
                     /locus_tag="PLF66_18275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HMZ66042.1"
                     /translation="MADLDKIKAIIVEQLGVDESEVTPEAHFIDDLGADSLDTVELVM
                     ALEEEFGIEISDEDAEKIQTVGDVAKYIDKAKS"
     gene            5940..6683
                     /gene="rnc"
                     /locus_tag="PLF66_18280"
     CDS             5940..6683
                     /gene="rnc"
                     /locus_tag="PLF66_18280"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HMZ66043.1"
                     /translation="MSRPTLKTSPNKQLPPERVKELKDLIKHIKIPFQDLVLLNSAFT
                     HKSFLNETHMEYEDNERLEFLGDSALSLVVSSYLYQRFPKDREGKLSKVKSRIVSGAV
                     LSKIAIQLGLNRYLLLGKGEKANGGDTNRSNLENVLEALLGAIYLEHGIQAVADFILP
                     HVGNIVDNELHSYKDHQTALQEFCQKKFKFIPDYELIAEEGPDHNKVFKVKVSIKNVG
                     EKIGMGNSKQKARQDAASKMLKYMKIKNA"
     gene            6676..7137
                     /locus_tag="PLF66_18285"
     CDS             6676..7137
                     /locus_tag="PLF66_18285"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004786001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HMZ66044.1"
                     /translation="MLDFFLKSKKLRVRVAALIRNNKGEIFLIKQKKKNKDYWLLPGG
                     GIEFGESAIDALARELKEELSLEMSTAKFLLINESIDPNGERHLIQLVFEVEVKNFNP
                     SIPKEEKAIIEFAFHSIDSVLQLELRPDIKNYFKETKKEKPRFIKSKWVEG"
     gene            7137..8231
                     /gene="aroB"
                     /locus_tag="PLF66_18290"
     CDS             7137..8231
                     /gene="aroB"
                     /locus_tag="PLF66_18290"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016755634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HMZ66045.1"
                     /translation="MQLSEQRVKFGNTDYKVVLYENFDGLSTELAKINNVSKFFILTE
                     KKIAKIYLKPLLSELEKLNIPVAVVFIKGKEKNKHIDRLKKVYNTLIDLNADRKSVLL
                     AFGGGVVGDFTGFVAATFLRGIRFVQIPTTLLACVDSSVGGKVAVNADKGKNMIGAFH
                     QPELVYAPLSTLTTLKKKEWKCGLAEVLKHSLLTGGDLWKFMKEAKFEDVYNENSLKV
                     FIEESVKFKTSIVAEDEKEIGKRAILNLGHTLGHAIESLTDYKKYSHGEAVSIGLTLA
                     LILSKEKSGLNESTFYEILQVMKNLDLPIQDKELSPEKLVKHMLHDKKTSENKIKYIL
                     LSEIGSASWGNIMQQEEILSAIYQQNNLSD"
     gene            8246..9145
                     /locus_tag="PLF66_18295"
     CDS             8246..9145
                     /locus_tag="PLF66_18295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010570507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HMZ66046.1"
                     /translation="MENLFELLEPFSIFKILSLPERKLSEDFILYLYIVAFLAFIYNI
                     ITFFLDRFTDDLDVQSIFTRRRVTRYTFFFFGLFCSIPIFYSKISYLPTILAFTGAGF
                     IISIKDITLNFAGWFLIHGSSGFAIGDRIEIGDVKGEVINIGLMRFTLLEVKTDHNSD
                     QSSNRLVHIPNHQAIIQKIFVISQDMEFIWDEVYIFLTTSSNWQKAKSICEKILSEVI
                     SHEEYSKIFENKIRRLSKNYLVRLGKITPIVYVTLEEGKVMLSLRYLTHFHHKRQNRA
                     FISEKILVEFKKEKSIHFFTHGI"
     gene            complement(9142..10182)
                     /locus_tag="PLF66_18300"
     CDS             complement(9142..10182)
                     /locus_tag="PLF66_18300"
                     /inference="COORDINATES: protein motif:HMM:NF014789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HMZ66047.1"
                     /translation="MQKLKDYLSSIPKDYDPENIYKDVLPLLVEENLLTVINQSISFK
                     DFHKNLFELAKLPHGIGLGVSLMAQVNIAGRIIFLAAKNLNHPVYLKLLNGLTKGEYI
                     ISLGVSEPDWKGRLSNIKTNITFNNGKYQLDGEKSFFTNGYHSTHFLVVARMDGNYKV
                     VLLEKENTGLEITKFTLPFAKEATHCRIKMNSVQINEEDILNLDYSKYAENLRLSEML
                     SLSTVFCGYAISTLEQIKANSKLVSILKEEEDKQKEILRCKTLVHMLYSRILELSELK
                     DNLEEIELKEFFPYGTELVSDEFYKTLSNIFPKEEIEPYFIEKQLFQYRDILNESYIR
                     RAARSASGLIKT"
     gene            complement(10252..11949)
                     /locus_tag="PLF66_18305"
     CDS             complement(10252..11949)
                     /locus_tag="PLF66_18305"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HMZ66048.1"
                     /translation="MSQCPNCETKLTTNAKFCYSCGFQLTKVNQEDDLSFLQRTLHPN
                     FIEIEKIGQGGMGSIFLGKQVSLNRKVVIKLLNEALCLDEKLVDNFLKEAQIAANLKH
                     PNIVEMVDFGKAEGRPFFIMEYGEKGSFEKILSETRTKNKKLDTIEVCKSIVKILYAL
                     DFAHSKNLLAHLDIKPHNIIQRDSGEIFITDFGIALDKRDKITKTRESIGTPEYMSPE
                     QIKGAKDIDARSDVYSVGILFFEMLTGFVPFESIEKEHIKEMHLKSEMPDLKPRFTPN
                     ELKKLEKENLNISDLTAIIHKACEKDREKRYFSCKEMAIAIESILYSIEEQKSESYKK
                     HRKQITFYGVTASILFTLILYFGARQKLEENIKESCTNCCKEGNCINGKGIFLYKPIR
                     EGARSNEYEGEFKEGKKHGYGKYFIYSMQAKYEGNFINDLYDGTGTLINFNDDELKDF
                     NSSYVGEFKANRPDGRGTYSFKNGSYFMAEFEDGKPKHDSITFITKEKSIYKGKFKDN
                     EGTILPDGKGILIFSDNRVYIGEFQNGEIHGTGMIILPNHKVLNGRAENGRFSPDKK"
     gene            12073..15171
                     /locus_tag="PLF66_18310"
     CDS             12073..15171
                     /locus_tag="PLF66_18310"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HMZ66049.1"
                     /translation="MQTPKTILLVDDDDVIATNHKAILVKEGYSVLTANTGEIAIQTA
                     LRESIDLVLMDIDLGTDIDGTEAARRILAKKEVPIVFLTSHSEKELVEKVKNITRYGY
                     VIKNSGAFVLLSSIEMAFELFYTQRETRRKEELFELIANNTLDLIALHNIDGSYIYLS
                     PSVERILGYKAEELIGENPYSLFHPEDIERIQKSSHEKVKVGEDGISISYRIKTKKGE
                     YIWLETISKSIWDENRENIIMLQTVSRDITERINKEKRENMYEAKLKESQARYKSMVE
                     SSFEAIFVHRDYKFIYANAAGLKLVGAKSFQELEGRSIFEFVHPEYIGQVEDRVHTLY
                     TEKEKAPIKEERFFRLDKTIIDVEISANPIEFDGEFSILVIARDITDKKNRERKMREA
                     EYRFQTLIESLEVGVIVQDSNAKILISNPAAERLLGLTRDQLKGKSSFDKDWNLIQED
                     GSDFPGEAHPVKICMKTKRPVTDVVMGVYRPKQQDRVWLLVNAKPEFGDNEELTQVIC
                     TFQDITARKQNDEILKEKEERLRLALAAGSQGLYDLDLRTGKATVNHFYSQMLGYDPN
                     DFNETNTFWKERLHEEDREGVYKIYLDYIAGVRTDYNVEFRQRTKNGDWKWILSQGKI
                     VERDKNGEPLRMIGVHTDITEKKKYELLQKARNSVLDSMIAKQDIYLILEEIVKNIEI
                     IQPDAKASILLIDKEKRLAKGASVSLPDFYNKAIEGLEIGNGVGSCGTAAFTGDLVIV
                     EDIETHAFWEPYKKIALEANLRACWSFPFKDDSGKVLGTFATYYNKPKHPSRSDLDII
                     WEFSRITGLAVQNSITEIERKKAEEEVSSLLAEKEILLKEVHHRIKNNMATISSLLGL
                     QSEYVNDERVSKILDDAQQRILSMMLIYDKLYRSSSDYQNISLDDYLNDLLDHIIVSF
                     PGGHLVSIERNIASFTLNSKILFSIGIIINELITNSFKYAFTGTRQKEGKIHVSAKKV
                     EGDKIELIVSDNGVGLPEGFSIKTSKGFGLNLIHILTQKKGNLFEVNGNGGTTFRVIL
                     SL"
     gene            15265..15495
                     /locus_tag="PLF66_18315"
     CDS             15265..15495
                     /locus_tag="PLF66_18315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015679594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66050.1"
                     /translation="MKVSIGNFLIALILGLFIGAVAGSLVDKILNLQMLSKALLSEAI
                     RFELYIIKIEIQLTPAALVGMVVTGYLVIRKG"
     gene            15498..16382
                     /gene="rpsB"
                     /locus_tag="PLF66_18320"
     CDS             15498..16382
                     /gene="rpsB"
                     /locus_tag="PLF66_18320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010418069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HMZ66051.1"
                     /translation="MSVISMKNLLEAGVHFGHQTRKWNPKMAPYVFTARNGIHIIDLQ
                     KTVQKAKEAYDALKRLTSQKKTVLFVGTKKQARGAIEREAKRCNMFYVSNRWLGGLLT
                     NWNTVKKSIARLKKLEQMLENNTFEQEAKTKKEHLGLKRELEKLKKTLGGIKDMNTIP
                     DILFVIDPKKEEIAVKEAKTLGLTVFAVIDTNCDPELIDYAIPGNDDAIRAISLFLET
                     MANAVIEGTGGEVPESAQFTDDMDVDMGMDYRGEYDEKGRFIMDDDATFEKEFKEVVA
                     SSVVPDEVHAMKDEEKEG"
     gene            16387..16983
                     /gene="tsf"
                     /locus_tag="PLF66_18325"
     CDS             16387..16983
                     /gene="tsf"
                     /locus_tag="PLF66_18325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016756586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HMZ66052.1"
                     /translation="MAEVSSELIKELRESTGAGLLDCKKALIENGGDMQKAINFLREK
                     GMAKAAKRVDKETKEGRIISYIHSNGKIGVLLELNCETDFVAKNEAFDELGRELCLQI
                     AASNPSYMNISDVPQDVIDNETKILKSQLLEQGKKPEQIDKIIPGKLTSFYTEVCLME
                     QQHIKDNKKTIKELVQESIAKFGENITIGRYTRYQIGG"
     gene            16984..17745
                     /gene="pyrH"
                     /locus_tag="PLF66_18330"
     CDS             16984..17745
                     /gene="pyrH"
                     /locus_tag="PLF66_18330"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020766681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HMZ66053.1"
                     /translation="MDSNQPNQHKPYKRILIKLSGEALAGEGEYGIDGNKTMALAKEI
                     ADIHRQNIEVAIVVGGGNIFRGIRGVEKGIERATADYMGMMATIMNALALQDACEKIG
                     LYTRVQSAIEVNSIAEPYIRRRAVRHLEKKRVVIFAGGTGNPYFTTDTTASLRAVEVG
                     CEVILKATKVDGVYTADPKIDKSAKRYTNVSFSESIRKRLKVMDQTALSLCMENNMPI
                     IVFDIFKQGNLADLISGKIVGTLISNSEEVNFANE"
     gene            17738..18286
                     /gene="frr"
                     /locus_tag="PLF66_18335"
     CDS             17738..18286
                     /gene="frr"
                     /locus_tag="PLF66_18335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HMZ66054.1"
                     /translation="MSDYVAQAKVKMDKTIDALKKDFLQIRTGRANPAMVEDIKVEYY
                     GAMTPLNQMGSITTPEPRLIMISPYDKSVIKNIEKAILTSGMGFSPNNDGAVIRIPLP
                     ELTGDRRKELVKVLKQKSEEKKVAIRNIRRDVNDDIKRDNAEASQDQVKGLTDQIQKL
                     TDSYIAKIQEMTDAKEKEITTV"
     gene            18301..19020
                     /locus_tag="PLF66_18340"
     CDS             18301..19020
                     /locus_tag="PLF66_18340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016756587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HMZ66055.1"
                     /translation="MKIKTKLPEHIAIIMDGNGRWATARGLPRKEGHKEGSLAIDRLM
                     DTAIEIGLKNISLYAFSTENWKRPVSEVRAIFGLLENFIDSRLEAIQSKNIQIKHSGS
                     RKMIPGSSLKRIDKAVERTNKNTGIVINFCLNYGSHDEILTAFNRVIQNRIQKKKSLE
                     KDIKQEELEAALYTHPLPPVDMLIRTAGEKRVSNFLLWQIAYAEIYFTDTLWPDFDRR
                     SLQDALDWYASRTRKFGGLEK"
     gene            19017..19934
                     /locus_tag="PLF66_18345"
     CDS             19017..19934
                     /locus_tag="PLF66_18345"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010569246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HMZ66056.1"
                     /translation="MKETLMRILSAVILLPIFIFSFQYSAFLYYMPLFLLGLTVIYLG
                     LNEFYKLSDKGEEGRPFRMTGMVLSILIFLIYYLNLLFRQNQVELPSGLASMVGYFKS
                     EYELVSIAVFLVAFFSFVWQILYRPLDGAIYSVSTTVLGVIYLAIPLGHFLKLLSLPH
                     GIYYIYIVCGLTMITDAGAYFGGRLFGRHPAGLKISPKKTIEGYVTGSVSAVLYCLII
                     NAIWVKGLGKNIPFGTIETIVFGFVFSVISVAGDLSESAMKRDAKTKDSAGTIPGHGG
                     VLDLADALLFTIPTCYVYLVLKEMVGFSI"
     gene            19943..>20983
                     /gene="dxr"
                     /locus_tag="PLF66_18350"
     CDS             19943..>20983
                     /gene="dxr"
                     /locus_tag="PLF66_18350"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020776147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HMZ66057.1"
                     /translation="MQRICLLGASGSVGESTLKVIRLFPESFGLYSFSVHSNLEKAKL
                     IIEEFSPSYAVVTSDEVDKTVLGNRYKSTRVLYGASAMSEIVKMTPVHIVVTAVVGSL
                     GVHPTIASIEAKKKIAIANKETLVTFGPYINSLLEKYKVTMVPVDSEHNALFQLVESQ
                     KKENIASLTLTASGGSFRDLPLEKLKNVTVEEALNHPTWKMGPKITVDSAGLINKGLE
                     VIEAHFLFGIPYENIEVVIHPESIVHGMLTLIDGATLMYASHPDMIYPIAHSLFYPKM
                     TPKVLIESKPPKWKKLNFWEPNAKRYPALALAYETGKKGGTAPALFNAANEEAVNLFL
                     NKKIQFTQIPEKI"
BASE COUNT         7330 a         3203 c         3851 g         6599 t
ORIGIN      
        1 aatataccta ttatgattta gaaagatggc gagaagaaat tgaaaataag aagattcaaa
       61 tcaatcagga aaatacttta aaggataaac ttctaaaaga gggtgatgaa atttctttta
      121 ctattccaga ttttatagaa ccagaaattg attcagatta ttctgtttta tacgaagatg
      181 aatattattt tgcaattggg aaatcaggaa atctgcctgt tcatcccgcg ggaaggtatc
      241 gaaaaaataa tctaactaca ttattagaat cagattctag actcccaaaa cctttttatc
      301 tgattcacag aatcgatcga gaaacatctg gaattgttct atttggtaaa tcaaaattag
      361 ccgcaagtaa attaggagaa cttttcgaaa aaaataaaat tcaaaagact tatcttgtct
      421 atgttgaagg ggtttttcct gaatctttag aagcaaaagg atttttggaa aaagacgaaa
      481 aatcaaagat tcgaaaaaag aaaaaattta cctttgagaa aacaaaagat attttgtggg
      541 aagtctatac agactttgca agagtagaag tagaaggaaa tatttcaaag attaaagcat
      601 ttccacacac aggaaagatt catcaaatca gggcaactct tcattctcta ggctatccac
      661 tcttagggga taagatttat ggtagagatg aatcctattt tttagaattt attgaaacgg
      721 gtaaagctag agaaaatgaa cggatcgaaa ggcaggctct tcacgccttt tccattacct
      781 ttcctcatcc ttttttaaaa aaagaaattt caattgagtg tgtagaacca ttagatatgc
      841 aaaaaatatt ttctaactca ccttacataa aattgacact ttaaggaaaa agaaatgatt
      901 ttaataatat tatgaaatgc gaatgaacta ataaaatcaa tagcctgctt tatgtttcga
      961 ctgttagaga gaaacattga gtttggaaag ccctccgttc cttctctttc gctctgctca
     1021 agctcattgt catttttcaa tgacaatagg agcaaacctc atttgaacgc ttggctcacc
     1081 caaactcaat cattcctttt attaagcaat gatattgtaa ggtgcgtttc taaataatat
     1141 ccgatagtct ccacatctga gatagaatcg aacctttaga aaaattatct atattgggtg
     1201 ctccagataa atcactggta tcaatgactc taaaatctat actaaatctt gttttattgg
     1261 tttgatttgg tcttgttcca tgcagatgag aagcggaaaa aatcaacagt tctcctgcct
     1321 gcataggaat ttttaattca gaagtttgcg gtaaatccac tttagcctct ggaaaaattc
     1381 tctgggtttc aaacttgggg tttgcctgaa agccaccact caaattccaa ttttccaaat
     1441 gaaataaatt cgaatcattt tggataggtt tatcaaaata ttctggatag aacgcgaaag
     1501 tatctttctc atctacagaa aagataggaa tccaaaaatt gatttgtgtt tctgggtttg
     1561 cataccaagt gtctctatga attgcaaaag ctggcttagc attatcaatg gtatgtaaat
     1621 aagaagtaac acatcttagc cgtaaattgt ctatatatgt agtttcaatc ttgtaattga
     1681 attcttttaa gaggtttgct attcgtttct ttattgttaa attttttgta agctcttctt
     1741 ttatttcaga aaataattga tgaaactttt cgggggatac ttttttttcg aaggtttgta
     1801 aatcttttgt gcataatttt ttttctagaa aaaggtaaac aaagtctttt aaaacataag
     1861 aagatagaat atccttttta tgtaaaattt taccattaaa tatttctttt ttgaattgat
     1921 gcatcatgtt tgggaagtta tgaaaatact atataaatat ttctttgaaa aaaattccac
     1981 tctgaaatta tacaaaatat gaattggcaa tctaagtctt cacccttaaa atcatcaact
     2041 atcagaacac tcatttgttt tctaatatag atttaaaata ctatatatca gtttaagctg
     2101 tatcaaatcc ctacagtgaa ggataaagat ttagtgatga tataataata tatactgtat
     2161 ggtaaatgaa aataatttag aatcatttga aatcgtttga ctaattcgaa ttgaattaaa
     2221 atccttggtg tatgaatcaa agaaaattaa gaatattatt ttatacaatg agtatatttc
     2281 tattggggct aacggtagtt tgttccagtc ctaaagtaga aatcccgaag ggaacagaac
     2341 ttggtaagga atacccaaat ccaaatgaag aagaaatcgc aaagagaact ctggagttga
     2401 ctttaacttc tatcaaggaa aatgcaaaaa acaataacgg taaggtctta agagatgctc
     2461 atccaaaaca tcacggatgt gtttctggat catttactgt ggaaaataac gttcccggaa
     2521 tgatgaagca tgggatattt tctaaaccta aatcctatcc aatctggatt cgcttttcta
     2581 atgggggaac tccaccaaaa gaggataaag agggcgatat ccgtggtatg gcaatcaaac
     2641 taatgggtgt agaaggggaa aaattgttag aggatgagta aaatgaaaag actcaggatt
     2701 ttttattaat cagccatact gttttacctg ttggagatcc aaaagattat ttagatttat
     2761 ttgaagctgc atttgccaaa aagccactcc ggcatttaat ctttagcggt caatttggag
     2821 cgattcgaaa gttaggcgcg ataagaggac ttaaagttcc atctcccctt ggaattcgat
     2881 attttagcac aacaccttat aaattaggag ctggggctgt taaatattcc gctaaaccat
     2941 gcaatggatt taatccacct atgcctgcgg agctaacacc aaattacctc aaagaaacaa
     3001 tgataaatca tttgaatgag aaagaagctt gttttgattt tatgatacaa ccacaaaccg
     3061 atccaatcac tatgccggta gaagatccag cagaagagtg ggatgaaact gtttcgcctt
     3121 ttgtaaaagt tgctacaatt aaaattgata aacaaaaatt tgattcaacg gaacaaatgg
     3181 aagcctgtga aaatctttcc tttactccat ggcactcttt accagatcac aagccattgg
     3241 gagggatcaa tcgtgttcgt aaatctgtat acataggaat ttcaaaatat cgtcatgaga
     3301 cgaatggtgt gcctagaaaa gaaccaacaa aataagaatt gcatgagata atggattgtg
     3361 taaatgcttt aaaaaaaatc taaccacgaa gagcacgagg gtcgagaagg tcaaaatttc
     3421 cttctaaatt tacaatttta cttcgtggca ttcatggtga aaatcttgct tttacacagt
     3481 ccgaaataag aaagcgctca atttaaggat taccgcacag gctcacggat agtggtaatt
     3541 tttttccaat ctaaacattc taaatttatc caatgagttt aataattgcc acatagattg
     3601 aaataatatc taatgcaaag aaaggataaa ttatgaacga accactcaat cattctgtaa
     3661 aagatatcaa tgggaatcta gtaaatctaa atgagtttaa gggaaaaact acactcattg
     3721 taaatgttgc ttctaaatgt ggactcactc cacagtataa aggcttacaa gagttatatg
     3781 ataaatacaa agataaaaac tttgtgatcc ttggttttcc gtccaatgat tttttgggac
     3841 aagaaccagg aacagaaaat gatattaaac agttttgtga acttaattat aaagtaagtt
     3901 ttcctatgtt ttcaaaaatc aaagtaaaaa agggagatgg tcaggctgat ttgtataaat
     3961 atctcacatc aaaagaaaca aatccaggct ttgagggaga gatagaatgg aactttcaaa
     4021 aattcttagt agataaaaat ggaaaaacag tgaaacgtta ttctcctaaa actacgcctg
     4081 aagaaatcgc gaaagaaata gaaaactatt tataactaaa gagaattgaa aaagaaacca
     4141 cctaggaatg acgcctttgt atatgttcat ctgcggtaaa ttatccgaat cgttaggact
     4201 gtgactcgga agagagtata aagactggaa tactatctcg ttttctgcta ttctttccct
     4261 ggtggtaata taattaaaaa aagaatttta aataaaataa attatgttta aagaagaatt
     4321 atttattaaa aaaacacctt accgcaaaaa acagataatc gctgttagcc tcttatttct
     4381 ttctggcatc tcattaattc tattagagac aaaattgatt ctagcgaatc aagagatgat
     4441 tcgaaaaatt ctaatcaatg catttgaagg gggatttgta ggtgggcttt gtgattggtt
     4501 tgcagtttgg aaaacgtaca atgcaatcga aaacgatagc ttgattgtcg cagatgaaat
     4561 agggcaatgg gttgcttctg atttattaga caaaaatacc ttaaaaaacc aaatcaacga
     4621 agtcttagac agtgctgaga ccaatgcaga aatttataaa cttctagata catattttga
     4681 cacaaaggaa aatacaaaga aaattttaga aaacctttgg attaagatgg agccttctgt
     4741 cattgagtat atcgttcatt acaatttttc tctcagtgaa atgtctctga ttacaaatgc
     4801 gtcatccgat cagattatca ttaatacaat taagatttgt gtaggggata ctttatctaa
     4861 aatttcggaa gaacagaagt ttaaagagtt tattgctaaa attgtaagcc aacagaatgc
     4921 aatgactaaa tttgtaagta tttttatcaa tattccaagt ataatcaaga tgtatggaga
     4981 aaaattgaaa tcgggagacg atagtttttc tgaaaacgaa caatttttgg atgaaatggt
     5041 tactataatt tcaatgagcg cagataaata tattctatca tggcaatccc tcagcacaga
     5101 aagaaaaaca gttgctgtga aagcgttgat tactcaactt agggaaacat ctctagatgt
     5161 tgctgcaaag tatatcattc tgcataaaaa tcatatcaaa gcctctaaga ccttgcgtaa
     5221 ttacatgcca gtcagggaat tttttgagtt tattgaagat aaaattgatg atcaagtacc
     5281 acagtttata ggtcataaaa tatccgaaag actcaaaagc caagatccta aagactttcg
     5341 agtaaatatt gaatggaaaa caagaaatat attggagaat attcgaatta atggaacgat
     5401 tctaggtttc tttcttggac tactgattgg agttttaaaa tattctatct aaataatttt
     5461 ttttaaaagg tgaaaaaaaa aattattaag acttgccaaa attttttaaa ttatagagcg
     5521 tcaattttga gatacataga gtatccttta acctttaatc tattacattt ttttgattat
     5581 gaaatcatac tttaaaatta gtagaaccaa ttaaaggtat tagccggagg taaaaaatgg
     5641 cagatttaga caaaattaaa gcgatcatcg ttgagcaact cggagtagat gagagtgaag
     5701 ttacacctga agcacacttt attgatgatt taggtgctga ctctcttgat acagttgagc
     5761 tcgttatggc attggaagaa gaattcggaa tcgaaatctc tgatgaagat gcagaaaaga
     5821 ttcaaactgt tggcgacgta gctaaataca tcgacaaagc aaaatcctga tccgatttta
     5881 ttttataata cgaagttaat aagaaattat tagcttcgta ttattttcca ctcccacttg
     5941 tgtcaagacc taccttaaag acttcaccta acaagcagtt accccccgaa agagtaaaag
     6001 aactaaaaga tttaatcaaa catatcaaga taccgtttca agatctggtt ttactaaatt
     6061 cagcttttac acataaatct tttttaaatg aaactcatat ggaatatgaa gacaatgagc
     6121 gtttagaatt tctaggagat tctgctttga gtctagtagt gagtagttat ctatatcaaa
     6181 gatttccaaa agatagggaa ggcaaacttt cgaaggtgaa atctagaatt gtatcaggcg
     6241 cagttctctc caagattgca attcaattag gactcaatcg atatttactc cttggaaaag
     6301 gagagaaagc aaatggtggt gatacaaatc gaagcaattt agaaaatgtt ttagaagctc
     6361 ttttaggtgc aatttattta gagcatggaa ttcaagcagt ggcagatttt attttaccac
     6421 atgtaggaaa cattgtagac aatgagcttc attcttataa agatcatcaa acagctttgc
     6481 aagaattttg tcagaaaaaa tttaaattta tacctgatta tgaattgata gcagaagaag
     6541 gacctgatca caataaagtt tttaaagtaa aagtatctat taaaaacgtt ggagaaaaga
     6601 ttgggatggg aaatagtaaa caaaaagcac ggcaggatgc ggcaagcaaa atgctaaagt
     6661 atatgaagat taagaatgct tgatttcttt ttaaaatcta aaaaactaag agttagagtt
     6721 gctgcactga ttcgcaataa taaaggcgaa atatttttaa ttaaacaaaa gaagaaaaat
     6781 aaagactatt ggcttttacc gggtggggga attgagtttg gagaaagtgc gatagatgct
     6841 ttagctagag aattaaaaga agagcttagc ctagaaatga gcactgctaa attccttctg
     6901 atcaatgaaa gcattgatcc aaatggagaa cgtcatttga ttcagcttgt tttcgaggta
     6961 gaagtaaaaa attttaatcc ttcaattcct aaagaagaaa aagcaattat cgaatttgca
     7021 tttcattcta ttgattcagt tcttcaatta gaactgagac cagatataaa aaattatttc
     7081 aaagaaacca agaaagaaaa acctagattt attaaaagca aatgggtgga aggataatgc
     7141 aattatcaga gcaaagagtt aagtttggaa atactgatta taaagttgtt ttatatgaaa
     7201 attttgatgg attgtcaact gagttagcaa aaataaataa tgtctcaaag ttctttattc
     7261 ttaccgaaaa aaaaattgca aagatctatt taaaacctct cttatctgaa cttgaaaaat
     7321 taaatattcc agttgctgta gtttttataa aaggcaaaga gaaaaataaa cacattgatc
     7381 gtcttaaaaa agtttataat acactcattg atttaaatgc agatcgaaaa tctgttttac
     7441 ttgcatttgg aggtggggta gtcggtgact ttacaggttt tgttgcggct acttttctaa
     7501 gaggaattcg attcgtgcaa attccaacta cacttctcgc ttgtgtggat tcctctgttg
     7561 gaggtaaggt tgctgtgaat gcagataaag gcaaaaatat gattggagcc tttcatcaac
     7621 cagagcttgt ctacgctcct ctctctacac tcactacctt aaagaaaaaa gaatggaaat
     7681 gtggactcgc agaagtttta aaacattctc ttttaactgg tggtgatctt tggaaattta
     7741 tgaaagaggc aaaatttgaa gatgtctaca atgagaactc actaaaagtt tttatagagg
     7801 agtctgtaaa atttaaaacg agtatagtag cagaggatga aaaagaaatc ggtaagagag
     7861 caattttaaa tttaggacat actcttggtc atgcgattga atccttaact gattacaaaa
     7921 aatattcgca cggagaagcc gtttccattg ggcttacact tgccctgatt ctttctaaag
     7981 aaaaatcagg tctaaacgag agcacattct atgaaatttt acaggttatg aagaacttag
     8041 atttaccaat tcaggataaa gaattatctc cagaaaaatt agtaaaacat atgttgcatg
     8101 ataaaaaaac aagtgagaat aaaataaaat atatattact ttcagagatt ggatctgctt
     8161 cttggggaaa tataatgcaa caagaagaga ttctttctgc tatttatcag caaaataatt
     8221 tatctgatta acagttatta aatgcatgga aaatctattc gaattattag aaccatttag
     8281 tatttttaaa atcttatctc ttccagaaag gaagctttcg gaagatttta tattatattt
     8341 atacattgta gcatttttag cattcattta taatatcata actttttttt tagatcgatt
     8401 tacagatgac ctagacgttc agagtatttt tacaagaaga agagttacgc gctatacctt
     8461 tttcttcttt ggtttgtttt gtagtattcc cattttttat tctaagattt cttatttacc
     8521 cacaatcctt gcatttaccg gtgcaggttt tattatttcc attaaagata ttactttgaa
     8581 ttttgctggt tggtttttaa ttcatgggag tagtggtttt gcgattggag acagaattga
     8641 gattggagat gtaaaaggag aagtgattaa tatcggtctt atgcgtttta ctttacttga
     8701 agttaaaaca gaccataatt cagatcaatc gtcgaatcgc cttgtgcata ttcccaatca
     8761 tcaagctatc attcaaaaga tatttgtgat ttctcaagat atggagttta tttgggatga
     8821 ggtgtatatt tttctaacta caagttctaa ttggcaaaaa gcaaaatcaa tctgcgagaa
     8881 gattctttca gaagtaattt cgcatgagga atattctaaa atatttgaga acaaaattag
     8941 acggctatca aaaaattatc tagttcggtt gggaaaaata actccaattg tatatgttac
     9001 attagaagag ggaaaggtaa tgctttctct acggtattta acgcattttc atcataagag
     9061 acagaataga gcttttatca gtgaaaaaat attagtagag tttaaaaaag aaaaatccat
     9121 tcactttttt acacacggga tttaggtttt aattaatccc gatgcagatc tagcggctct
     9181 tctaatgtag gattcattca gaatgtccct gtattgaaac aattgttttt ctataaaata
     9241 gggctctatt tcttcttttg gaaaaatatt agagagagtt ttgtaaaatt catcagatac
     9301 tagttctgtt ccataaggaa agaattcttt taattcaatt tcttctaaat tgtctttgag
     9361 ttcagataat tctaggattc tagaataaag catatggact aacgtcttac atcgaagtat
     9421 ttctttttgt ttatcttcct cttcttttaa gatagaaacc aatttagaat ttgctttgat
     9481 ttgttctagt gttgaaattg cgtatccgca aaagacagtt gaaagagaaa gcatttcgga
     9541 aagtctaaga ttttcagcat atttagaata atcaagattc aaaatatctt cctcattgat
     9601 ttgaactgaa ttcatcttga ttctacaatg ggtagcttct tttgcgaacg gaagagtaaa
     9661 ttttgtaatt tcaagtccag tattttcttt ctctaataga actactttgt agtttccatc
     9721 cattcttgct acgactaaga aatgagtcga atggtaacca tttgtaaaaa aggatttttc
     9781 tccatctaac tggtatttgc cgttattaaa tgttatattt gtttttatgt tagagagtct
     9841 tcctttccaa tctggttcag atactccgag agagattata tattcccctt ttgttaaacc
     9901 atttaataat tttaaataga ctggatgatt taagtttttt gcagctaaaa aaataattct
     9961 accggctata ttcacttgtg ccataagaga aacacctagc ccaatcccat gaggtaattt
    10021 tgctaattca aaaaggttct tatgaaaatc cttaaaagaa atactctgat tgattactgt
    10081 tagcaaattt tcttctacta gaagcggtaa tacatccttg taaatatttt ctggatcgta
    10141 atcctttggt attgatgaga gatagtcttt tagtttctgc attttcgaat cctttgaatt
    10201 gaatacattc tacaaattta aatatcgagg ctaacatacg taaaccgcaa tttatttttt
    10261 atcaggagaa aatcttccat tttctgctct tccgtttaga actttgtgat tgggtagaat
    10321 aatcatacct gttccatgta tttcaccatt ttggaattca cctatataaa cacggttgtc
    10381 tgaaaagatt aggattcctt ttccgtcagg tagaattgtt ccttcattgt ctttaaattt
    10441 tcctttgtaa atggattttt cttttgtgat aaatgttata ctatcatgct ttggtttgcc
    10501 gtcttcgaat tcagccataa aataagatcc attcttgaaa gaatatgtgc ctcgtccatc
    10561 tggcctattt gctttaaatt caccaacata gctactatta aaatctttta attcatcatc
    10621 attgaaattg attaacgttc ctgttccatc gtataaatcg tttataaaat ttccttcata
    10681 ctttgcttgc attgaatata taaaatattt tccatatcca tgcttttttc cttctttgaa
    10741 ttcgccttca tattcattgc ttctagctcc ttcacggatt ggtttatata gaaatatacc
    10801 tttcccattg atacaatttc cttccttgca acagttagtg cagctttctt ttatattttc
    10861 ttctaacttt tgccttgccc caaaatacaa aataagtgtg aatagaatac tcgctgttac
    10921 tccatagaaa gtgatttgtt ttctgtgctt tttataggat tcagatttct gttcttctat
    10981 agaatataaa atggattcaa ttgcgattgc catttcttta caactgaaat aacgtttttc
    11041 tctgtctttc tcgcaggctt tatgaataat agccgttagg tcactgatat ttaaattttc
    11101 tttttctagt ttttttagtt cattcggggt aaatctaggt tttaggtcag gcatttctga
    11161 ttttaaatgc atttccttta tatgttcttt ttcgatagat tcaaacggaa caaagccagt
    11221 aagcatttca aaaaataaaa taccaactga gtatacatct gatctagcat caatatcttt
    11281 tgccccttta atttgttcag ggctcatata ttcaggtgtt cctatgcttt ctcttgtttt
    11341 agtaatctta tctcttttgt ctaatgcaat accaaagtct gtgataaata tctcaccaga
    11401 atctctttga attatattat gtggttttat atctaggtga gcaagaagat ttttagagtg
    11461 tgcgaagtct aacgcatata atattttaac aattgattta caaacttcaa ttgtatctaa
    11521 tttcttattt tttgttcttg tttcagatag gattttttcg aaggaacctt tttctccgta
    11581 ttccataata aaaaatggtc ttccttctgc tttaccgaaa tcaaccattt ctactatatt
    11641 aggatgtttt aagttagccg ctatttgagc ttcttttaaa aaattatcta ctagtttttc
    11701 atctaaacag agagcttcat ttaaaagttt aataacaact ttacgattga gggaaacctg
    11761 cttgcccaaa aaaattgaac ccattccccc ttgacctatt ttttcaatct caataaagtt
    11821 gggatgcaag gttctttgta aaaaactaag atcatcttct tggtttactt ttgttaattg
    11881 aaagccacag gagtagcaga actttgcatt tgtagttaat tttgtttcac aatttggaca
    11941 ttgactcata ctaactgact aaattgattt taatttgcag tcaagagttt tggatatgga
    12001 ttgtagtttt ttagaaggta atcatttttt tgtttcaaag aaattaggaa tataataata
    12061 ttcctattga acatgcaaac tcccaaaaca atacttctag tagatgacga tgatgtgatt
    12121 gctactaatc ataaagcaat actagtaaaa gaaggatatt ctgttctaac cgcaaatacg
    12181 ggtgagattg cgattcaaac ggcactcagg gagtctattg atttagtatt aatggatatt
    12241 gacttaggga ctgacattga tgggacagag gctgctagaa gaatattagc taaaaaagaa
    12301 gttccaattg tctttcttac ctctcactca gaaaaagagt tagttgaaaa ggttaaaaac
    12361 atcacccgtt atggctatgt aataaagaat tcaggtgcct ttgttctctt atcctctatt
    12421 gaaatggctt ttgagttgtt ttatacacaa agagaaacaa gaagaaaaga agaattgttt
    12481 gaactcattg caaacaatac gttagaccta atagctctcc ataatataga cggtagttat
    12541 atttatctca gtccttctgt tgaaagaata ctaggctata aagcagaaga actcattgga
    12601 gaaaatcctt attctttatt tcatccggaa gatatagagc gaattcaaaa atcatctcac
    12661 gaaaaagtaa aagttggaga agatggaatt tctatttcct atcgaatcaa aacaaaaaag
    12721 ggtgaataca tttggctcga aacaatctcg aaatcgattt gggatgaaaa tagagaaaat
    12781 attatcatgt tgcaaacggt ttctcgggac atcactgaac gaatcaataa agagaagcgg
    12841 gagaatatgt atgaagccaa gctcaaagaa agccaggcta gatacaagag tatggtcgaa
    12901 tcttcttttg aggctatatt tgttcacaga gattataaat ttatttatgc aaatgcagca
    12961 ggattaaaac ttgtgggagc aaaatctttt caggaattag aaggtagaag tatttttgag
    13021 tttgttcatc ctgaatatat aggacaagta gaggatagag ttcatacact ttatacagaa
    13081 aaagaaaaag ctcctattaa agaagaacga ttttttcgtt tagataaaac aattatagat
    13141 gtagaaattt ctgccaaccc aattgagttt gatggagagt tttccatttt agtcattgct
    13201 cgagatatta cagataaaaa gaatcgagaa cgcaaaatga gagaagcaga gtatcgtttt
    13261 caaacgttaa ttgaaagtct agaagttgga gttatcgttc aagattcaaa tgcaaaaata
    13321 ttgatcagta accctgcagc agaaagatta ctaggcttga ctcgagatca actaaaggga
    13381 aagtcctctt tcgataaaga ttggaatttg attcaagaag atggatctga ttttcctgga
    13441 gaagctcatc ctgttaaaat ttgtatgaag actaaaagac cagttacaga tgttgtgatg
    13501 ggggtctata gaccaaaaca gcaagatagg gtttggttat tagtcaacgc aaaaccagag
    13561 tttggtgaca atgaggaact aacgcaggta atttgcacat ttcaggatat tactgccaga
    13621 aaacaaaatg atgagatttt aaaagagaaa gaggaaagac tgaggctggc attggctgct
    13681 ggtagtcaag gattatacga cttagattta agaactggaa aggcaacagt taatcatttt
    13741 tactcacaaa tgcttggtta tgatccaaat gattttaatg aaacaaatac tttttggaaa
    13801 gagcgtttgc atgaagaaga tagagaggga gtttataaaa tctatttgga ttatattgca
    13861 ggtgtaagaa cagattataa cgtagagttt agacaaagga caaagaacgg agattggaaa
    13921 tggattttat ctcaggggaa gattgtagaa agagataaaa atggagaacc tcttcggatg
    13981 atcggtgttc atacagacat cacagaaaaa aagaaatatg aattacttca aaaagcaaga
    14041 aattctgtat tagattctat gatcgccaaa caagatattt atttgatact tgaggagatt
    14101 gttaaaaaca tagaaatcat acaaccagat gcaaaggctt ccattttact cattgataaa
    14161 gaaaaacgtt tagcgaaagg agcaagcgtt agtttaccag acttttataa taaagctatt
    14221 gaaggattgg aaattggaaa tggggtaggg tcttgtggaa ctgctgcatt caccggtgat
    14281 ttagtgatag tggaagatat tgaaacgcat gccttttggg agccgtataa aaaaattgca
    14341 ttagaagcaa atcttagagc ttgttggtcg tttccgttca aagatgattc tggcaaagtt
    14401 ttaggaacat ttgcaactta ttataataag ccaaaacatc caagtcgatc tgatttagat
    14461 atcatttggg aattttccag aattactgga cttgctgttc aaaatagtat aacagaaata
    14521 gaaagaaaaa aagcggaaga agaagtaagt tctttgttag ccgaaaaaga aattctacta
    14581 aaagaagttc atcacagaat taaaaacaat atggcaacca tctctagttt gcttggactg
    14641 caatctgaat atgtaaatga tgagagagtg agtaagatat tagatgatgc acagcaaaga
    14701 atattaagta tgatgcttat ttacgataag ctatatagat cgtcttcgga ttatcaaaat
    14761 atttctttgg atgattattt aaatgatcta ctagatcata ttattgtttc ctttccaggg
    14821 ggacatttgg tttcgataga aagaaatata gcctccttta ctttaaattc taaaatatta
    14881 ttttcgattg gaattatcat caatgaatta atcacaaata gttttaagta tgcctttact
    14941 gggacgagac aaaaagaagg gaagattcat gtatctgcca aaaaagtaga aggggataag
    15001 attgaactta ttgtttctga taatggagtc ggattaccag aaggattttc cataaaaact
    15061 tctaagggtt ttggtttaaa tctaattcat atccttacac aaaaaaaagg aaatcttttt
    15121 gaagtaaacg gaaatggggg aactaccttt agagttatac tctctcttta gaaaagtttc
    15181 tctaagccta tctctttcga attagttgac cataaaccct attgattttt actggttaaa
    15241 agtccatcga atgcatgaaa ttctttgaaa gtatctatcg gaaattttct aatagctctc
    15301 attctaggtc tcttcatcgg ggcagtagcc ggatcattgg ttgataaaat tttaaatcta
    15361 caaatgcttt ctaaggcact tctctccgaa gccattcgct ttgaacttta tattattaag
    15421 atcgagatac agttaacccc tgccgctcta gttgggatgg tagtaacagg ttatctcgtg
    15481 ataagaaagg ggtaaaaatg tcagtaattt caatgaaaaa cctgctagaa gcaggcgttc
    15541 actttggcca ccaaactcgt aaatggaatc caaaaatggc tccatacgtt ttcacagcga
    15601 gaaatgggat tcacatcatc gacctacaaa aaacggttca aaaagcaaaa gaagcttatg
    15661 atgctttaaa gagattaacc tctcaaaaga aaactgtttt attcgtagga accaagaaac
    15721 aagctcgtgg tgcaatcgaa agagaagcaa aacgatgcaa catgttttat gtatccaatc
    15781 gttggttggg tggactttta acaaactgga acacagttaa aaaaagcatc gctcgtttaa
    15841 aaaaattaga gcaaatgcta gaaaacaata cattcgaaca agaagcaaaa actaaaaaag
    15901 aacatcttgg attaaaaaga gagttagaaa aactaaaaaa gactctcggt ggaatcaaag
    15961 atatgaacac aattccagat atcctttttg tgattgatcc aaagaaagaa gaaattgcag
    16021 tgaaagaagc aaagactctt ggactcacag tatttgctgt aattgataca aactgcgatc
    16081 ctgaactaat cgattatgca attcctggta acgatgatgc gatacgcgct atttctctct
    16141 ttttagagac aatggcaaat gctgtaatcg aaggaaccgg tggagaagtt ccggaatctg
    16201 ctcaattcac agacgatatg gatgtggata tgggaatgga ttatcgtggc gagtatgatg
    16261 aaaaaggtcg tttcattatg gacgatgatg caacatttga aaaagaattc aaagaagtag
    16321 ttgcttcttc tgtagttcca gatgaagtgc atgcaatgaa agacgaagag aaggaaggtt
    16381 aataagttgg cggaagtatc atctgaatta attaaggagc tccgtgaatc aaccggagca
    16441 gggttattag attgtaaaaa agctctcatt gaaaatgggg gcgatatgca aaaagcgatt
    16501 aactttctac gtgaaaaggg aatggcgaaa gcagcgaaga gagtagacaa agaaaccaaa
    16561 gaaggaagaa ttatttctta cattcacagc aatggaaaaa ttggagtgct actcgaactc
    16621 aactgtgaga cagactttgt tgctaaaaac gaagcatttg atgaactagg aagagaactt
    16681 tgcttacaaa ttgcagcttc taatccaagc tacatgaaca tttctgatgt tccacaagat
    16741 gtaattgata acgaaacaaa aattttaaaa tcccagcttt tagagcaagg taaaaaaccg
    16801 gaacaaattg ataaaattat tcctggaaaa ctaacaagct tttatacaga agtttgtctg
    16861 atggagcaac aacacattaa agacaataaa aaaactataa aagagctagt ccaagaatca
    16921 attgcaaagt tcggtgagaa catcacaatt gggagataca ctcgttacca aattggaggc
    16981 taattggact caaatcaacc gaatcagcat aaaccctata agagaatatt aattaaatta
    17041 tcgggagaag cactcgcagg tgagggtgag tatgggattg atggaaataa gaccatggct
    17101 cttgccaaag agattgctga cattcataga caaaacattg aagtagcaat cgtggttggt
    17161 ggtggaaata tctttcgagg cattcgaggt gttgagaaag gtattgaacg agctaccgct
    17221 gattacatgg gaatgatggc aaccatcatg aacgcactag cactgcaaga tgcatgtgag
    17281 aaaatcggtt tatatactag agttcaatct gctatcgaag taaactcaat tgcagaacca
    17341 tatatacgaa gaagagctgt tcgccactta gaaaaaaaac gagtggtgat ctttgcaggt
    17401 ggaacaggaa atccctattt caccacagat acaacggcta gtcttcgtgc tgtagaggtt
    17461 ggctgcgaag tcattttaaa agcaacaaaa gtagatggtg tatatacagc agatcctaaa
    17521 atagataaat ctgcgaagag atatacaaac gtttctttct cggaatctat ccgaaaaaga
    17581 ctgaaggtca tggatcaaac agctctgagt ctttgtatgg aaaataatat gccgattatc
    17641 gtatttgata ttttcaaaca aggaaatcta gctgatctta tttctggcaa aattgtcgga
    17701 acattaatct caaactcaga agaggtaaat ttcgcaaatg agtgattatg tggcacaagc
    17761 aaaagtaaag atggataaaa ccattgatgc attaaaaaaa gattttttgc aaattagaac
    17821 aggccgtgca aatcctgcga tggtcgaaga tataaaagta gaatactatg gagcaatgac
    17881 tccactcaat caaatgggat caatcactac accggagcct cggttaatta tgatttcccc
    17941 ttacgataaa tcagttatta aaaatataga aaaagcaatt ctaacttctg gaatgggctt
    18001 ctcaccaaat aatgatggtg ctgtgattcg aattccactt cctgaattaa caggcgatag
    18061 acgaaaagaa cttgtgaaag tattaaaaca aaaaagtgaa gaaaagaaag tagcaatccg
    18121 aaatattcgc agggatgtca atgatgatat caagcgggac aatgcagagg cttcacagga
    18181 tcaggtgaaa ggtcttacag atcaaattca aaaattaact gattcttata tcgctaaaat
    18241 ccaagagatg acagacgcga aagaaaaaga aatcaccact gtttaatagg cgatagagca
    18301 gtgaaaataa aaaccaaact tccagaacac atcgcaatta ttatggatgg gaatggtaga
    18361 tgggcaaccg caaggggact tcctaggaaa gagggtcaca aagaaggctc tcttgctata
    18421 gatcggttga tggacactgc aatcgaaatt ggactcaaga atatctccct ttatgctttt
    18481 tcaacagaaa attggaagcg acctgtttct gaagtaaggg caatttttgg attattagaa
    18541 aacttcatag actcaagatt agaagccatt caaagtaaga atattcaaat caaacattct
    18601 ggatctcgta aaatgattcc aggaagttcc ttgaaacgaa tcgataaagc agttgagaga
    18661 acaaataaaa atactggaat tgtgattaac ttttgtttga actatggttc ccatgatgag
    18721 atactgactg catttaatcg ggtcattcaa aacagaatac agaaaaaaaa gagtcttgag
    18781 aaggatatta aacaagagga gttggaagct gctctttaca ctcatcccct tcctcctgta
    18841 gatatgctta ttagaactgc gggagaaaaa agagtttcca attttctgct atggcagata
    18901 gcatatgcag aaatctattt tactgataca ctctggcctg attttgatag aagatctctg
    18961 caggatgcat tggattggta tgcaagtaga acaagaaaat tcgggggatt agaaaaatga
    19021 aagaaacatt aatgagaatt ttatcggcgg taatcttact tccaatattt atattttctt
    19081 ttcaatacag tgccttttta tattacatgc ctttattttt attggggcta acagtcattt
    19141 atctcggact aaatgaattt tataagctat cggataaagg agaagagggc agaccctttc
    19201 gcatgacggg aatggttctc tctattctta tatttttaat ctattattta aatctattgt
    19261 ttagacaaaa tcaagtggaa cttccatctg gtttggcttc catggttggt tattttaaat
    19321 cggaatacga actagtttcg attgcagtct ttctggttgc atttttttct ttcgtatggc
    19381 agattcttta ccgaccactg gatggagcaa tttattcagt aagcacaact gttttgggag
    19441 taatctatct tgcaataccg ctcggtcatt tcttaaaatt actttctctt ccacatggaa
    19501 tctattatat ctatatcgtc tgtggtctta ccatgattac agatgcgggg gcttattttg
    19561 gaggaagact ttttggaaga catcctgctg gtcttaaaat ttctcctaag aaaacaatag
    19621 aaggctatgt gacaggaagt gtgagtgcag ttttatattg tctaattatc aacgctattt
    19681 gggtgaaagg attaggaaaa aacattcctt tcggaacgat agagactatc gtatttggtt
    19741 ttgtattctc tgtaatcagt gtggctgggg atttatctga atccgcaatg aaacgagatg
    19801 caaaaacaaa agactcagca gggacaattc caggacatgg tggagtttta gatttagcgg
    19861 atgctctttt atttacaatt ccaacttgct atgtgtattt agttctcaaa gaaatggtcg
    19921 gttttagtat ttaggatttg ccatgcagag aatttgtctt ctgggtgctt ctggttccgt
    19981 aggagaatct acactaaagg tcatacgttt atttcccgaa tcgtttggac tctattcctt
    20041 tagtgttcat tctaatcttg aaaaagcaaa attgattata gaagagtttt cgccgagtta
    20101 tgcagtggta acatcggatg aagtagataa aacagttctt ggcaatcgct acaaaagcac
    20161 tcgtgttctt tatggagcat ctgctatgag cgaaattgta aagatgactc ctgtgcatat
    20221 tgttgttaca gcagttgtag gatctcttgg ggttcatcca acgatagctt ctatcgaagc
    20281 aaagaaaaaa attgcaatcg caaataaaga aacccttgtt acattcggac cttacataaa
    20341 ttctcttttg gaaaagtata aagttactat ggttccagtt gactcagagc ataacgcatt
    20401 gtttcaacta gtcgaatccc aaaaaaagga aaacattgct tcgcttacct taactgcatc
    20461 gggtggatct tttagagatt tgccactgga aaaattaaaa aatgttacag tagaagaggc
    20521 actcaatcat cctacttgga agatgggacc taagattaca gtcgattcag caggtctgat
    20581 caataaaggg ctcgaagtaa tagaagctca ttttctattt ggaattcctt acgagaatat
    20641 agaagttgtg attcatccag aaagtattgt gcatggaatg cttactctaa tcgatggggc
    20701 tactcttatg tatgcctctc atccagatat gatttatcca attgctcatt ctctctttta
    20761 tcccaagatg actcccaagg tattaatcga gagtaaacca cctaaatgga aaaagctaaa
    20821 tttttgggaa ccaaatgcaa aaagatatcc tgctttagcg cttgcctatg aaactggtaa
    20881 aaaaggtgga actgcgcctg cactttttaa tgcagcaaat gaagaagccg taaatctttt
    20941 tttaaataag aaaattcaat tcactcagat acctgagaaa att
//
LOCUS       DAOQOS010000096        20824 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_1239_length_20824_cov_5.407723, whole genome shotgun sequence.
ACCESSION   DAOQOS010000096 DAOQOS010000000
VERSION     DAOQOS010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 20824)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20824)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20824
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1239_length_20824_cov_5.407723"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            252..1523
                     /locus_tag="PLF66_18355"
     CDS             252..1523
                     /locus_tag="PLF66_18355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase C-terminal
                     domain-containing protein"
                     /protein_id="HMZ66058.1"
                     /translation="MPAGNLEKMKLAYEYGADAIYCGVPRYSLRARENDFTMEDLKSA
                     VLYAHSRNKKIYFTINAFPRNSKLPSYPKYLQQMAELKPDGFIMADPGLIFLTRKLAP
                     EINIHISVQANTMNYASVEFWKSVGATRVILSREVSISEVKEIREKVPDMELEVFVHG
                     AVCIAHSGRCLMSNYFTQRDANQGACNNACRDKYKVFVTNTRQNDEPMEIIESDEGTF
                     LMNSKDMRAIQYLKEIRDAGVDSIKVEGRTKNEYYVALVARSYRKALDDLKDGKEFDE
                     RLLDELNKVASRKYFSGFLTRGMEERVPVEEQNFQNYEIGYSYEQSQMYAGTIQKVLD
                     DSLASFQIKNKIALGEKMEVFFPRNFESKIFSIEEMYFKGEKKEVLSGGMGEVQIKIP
                     FPVEEHCILSVIRKSERKKDESNQKVTISIP"
     gene            1486..2118
                     /locus_tag="PLF66_18360"
     CDS             1486..2118
                     /locus_tag="PLF66_18360"
                     /inference="COORDINATES: protein motif:HMM:NF014543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66059.1"
                     /translation="MNQIKKLLFLFLSYNLIVLLILGLLNYSSGKLLSLPYSGILGSS
                     RWIGMLFGIFFINSIFSLGLIFVLQKMITKESFHENIVRNYLILTLVLMLWATFRLSN
                     TVCIDGNCKSGTGRLAYKTGEIYEGDIDDYQANGIGKIFYPNGDIYSGEMQEGKPNGK
                     GLLTLNNQNTKGSIEGIWFKGKLNGEAKRILEPENKIEILLYRDGIISHK"
     gene            2146..2814
                     /locus_tag="PLF66_18365"
     CDS             2146..2814
                     /locus_tag="PLF66_18365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66060.1"
                     /translation="MKTNTLFKFLLLSFIAIAIYSQNNDKPSSFFWTESQGKMTLSAA
                     KTKCASLGLRLPTRDEIISAHSKGETKTWKKDGELYWTLDGYSEEYGYSFDITKGSYG
                     YYEGKLERTVRCITSENNKVEVTTAKNIQKTKWSDYLGLMTWEDAKKKCESLGLRLPT
                     KEEMIQAYQNGETKSWEKEGEAYWTSTEFSKGTNSYYYFGVVNGFSSHYGGKDDDGHV
                     RCIQ"
     gene            complement(2891..5053)
                     /locus_tag="PLF66_18370"
     CDS             complement(2891..5053)
                     /locus_tag="PLF66_18370"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HMZ66061.1"
                     /translation="MQILKNKHLENVNFLGNIYLYAICFLFIPFLFSFIFYKLAIIAD
                     LSNKIIDVKYNYIERENLISKDIVLIEIDDKTLYELGDSPSFGRWPWKRNVYEPLIDY
                     ISSGSPKGILFDILFSEITENDKYLVNITKRNKNLSHAFYFNQLEEKTEKNSVEISKF
                     AVVSGNPLLTQNFSLEGAFPVESIRNVSSHMHGVNFKSDPDGVIRNYNFGYAAANHTY
                     PSLAWLALIFFEENLKLSIKKEEIIYQIKNHKIKLSHQNGFVPIHYYSKKSLDTLIRI
                     SVSDIFYSIYKMGSGEGIEKEENLILPDIFKDKIVLIGATSSGASDTHNSPYGELPGT
                     VIQAIVASNFLLGDFRNDLPFELEICIYLILSSISCYIFYHHYSLLKKVIFLGSTLLL
                     LFYLHIFLFRMNSNLPIQFGIIYLPSFSIVYGLVLLLEERERRKETRNILDKYVSKDV
                     VEDALSKRGVKLGEGQRREITVIFSDLRNFTSLSEQLSPEEVVQILNRYLGSMAKSIL
                     KRNGTLDKFIGDAVMAFWNAPNENTKHAEEAVKAAIDMFLSLSEINKQWKYNLKMGIG
                     IHTGPAIAGNIGSDERLDYTVIGDTVNLASRLESQTKIFGSDIIITDSTLKQLSFLER
                     IHYRYLGQITVKGKSEEISVFEIKTPFDEIELKNFKETKTDFEKAVQSFFNGNYRYSI
                     ELFHKVLKLYPNDKASSYYIERAETLLANPNMKHENLK"
     gene            complement(5034..5804)
                     /locus_tag="PLF66_18375"
     CDS             complement(5034..5804)
                     /locus_tag="PLF66_18375"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HMZ66062.1"
                     /translation="MRVIFFIIFLIYTCSSVENKTQESERVKQETLFGRQIVKNFLIR
                     YHLLHDRESTIALNSLVRKVAAFTSRNDLYYRAGILDTDDVFAFSIPGGYILISRGMI
                     KTWDSIESAEFVLAHELTHISLSHSFQPENNNSFEKFLQAGLDPTGGISVAANEFEKK
                     WDTMLQETGREPAQEFESDISAGYILIELGCNPLSALSYLEKMEKLTKRTDWIQKTHP
                     SFKDRVQVIQREFKNKSRKTNSCIGKEDWEKLNANFKK"
     gene            complement(5801..7225)
                     /locus_tag="PLF66_18380"
     CDS             complement(5801..7225)
                     /locus_tag="PLF66_18380"
                     /inference="COORDINATES: protein motif:HMM:NF015316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HMZ66063.1"
                     /translation="MIFSIQEVEADQVFQNIHSFLGERASGMGGAYTAVSDDATGTYY
                     NPGGLGFANKSSFTASSSGYQKGYDNYTNADGTGRDYIRKSSSYFPNFLGYVGGGEKL
                     KYGFSFVSPYQSQTDVANSITLPFQLYEGQYAYSNYVSINKEELIRTQFGLSLAYRLT
                     DRLSIGATLYYVRETHVLFATDEYEKFDKTRMREIYYDRKLSIGFEPILGFVYALTDK
                     LYLGFSTRKIINTAGRRNSYYSDENYFIYKSGFGSGGVDYTDSPFIQTKSNVSMPAPT
                     EIRTGLSYYFSEKLLWVGDFIYTSPYKLSGQNYIVNFSNPLRITFGDDEYHELRRTET
                     KNYATGLEYFVNQVFAIRIGYFTNHSSNKKIRWPQSVIESSIYEYASNRIAIDPANTI
                     YYAGIRGGHYQYDFNDAYYQNDVSSRGITLGFSYYISRNTSVSITVIHQRGRGGSVYY
                     FQAPSTPVLFLQDQVNFSITAMSE"
     gene            complement(7234..7686)
                     /locus_tag="PLF66_18385"
     CDS             complement(7234..7686)
                     /locus_tag="PLF66_18385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66064.1"
                     /translation="MKKLIQFFLLLSIFNLLPLGANDWVYISGKEPKLYQDPSLQTGI
                     KSVQADCRGKILKEESGKVFVDCSGIQGWILKIHTSPVSSAEKPLKLSESDFKRQSIT
                     RSRARLNDITGTLASRGFLYSDKDSTSIIQSEREAESSLRWLEKTTSE"
     gene            complement(7869..8552)
                     /locus_tag="PLF66_18390"
     CDS             complement(7869..8552)
                     /locus_tag="PLF66_18390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66065.1"
                     /translation="MTNNKSIVLISILIVSIVGYFAFFSGEKKKKRVESKKLSLLLGG
                     GGRGSEDGESASEKRRGRRNNNSSLFESDFMKSGISYQEEESTAANGEQGEIPINPQT
                     GKPWEEDAMQQFDELRKLFADNDLIPRRMSREDKAKQAQLEERWNKAQNAVNSGTYTR
                     DDLDTHFNHQKKVIEDRLQIIEYLIEAQKEDGEVDKDGQFQKILDGTKDQMKQLEAQR
                     EDLAKKLGG"
     gene            8645..9421
                     /locus_tag="PLF66_18395"
     CDS             8645..9421
                     /locus_tag="PLF66_18395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003007017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66066.1"
                     /translation="MLKFIKKLILLLGIPTVSFCGTNTDTPVVLAPLIFLTSPINVPQ
                     LVGVIPTQEYPTSGTGLSDLGGYVNPNAPQFIIKYFVTNTEQQFIGYNLSITSQTPTL
                     IETQAGTAGSVYTENGIQPSFPHLATENSTAKENMKRRRIAWRVPPPGIVYFQKCEMY
                     NFTLRAVLSNVPQTSNPSAAVSACAGIDPSKCPVGSSCNPSSCTDTQCSLTIRATCPV
                     GTLCNPCTILDSGGVAKAGCECPDGVSPNLYASPIVSCNR"
     gene            9421..10128
                     /gene="rsmI"
                     /locus_tag="PLF66_18400"
     CDS             9421..10128
                     /gene="rsmI"
                     /locus_tag="PLF66_18400"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004503928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HMZ66067.1"
                     /translation="MKNGSLYIVGTPIGNLEDITLRAIKTLNQVDLILCENAKNSQKL
                     LNHHQIKTPYKTLFTNTDSAYDWIIEDLKSGKEMAYISDAGTPGVSDPGAGLVRSVRK
                     AGISIVPIPGASALTSMLSISGYQTNPTYFLGFLSEKPNKKRTELAEYTEKDCVIVFY
                     ESVYKIKTTLSILQELFPDSEILIGRELTKIHEEILLYKPEDLPIESITLKGEFVLLI
                     NNHRKKIAKEKIILTDT"
     gene            10150..10446
                     /locus_tag="PLF66_18405"
     CDS             10150..10446
                     /locus_tag="PLF66_18405"
                     /inference="COORDINATES: protein motif:HMM:NF016223.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HMZ66068.1"
                     /translation="MFIDKIKNIGSAFEPKKSASVKKSSEILPSDNVQISDAAKLKAD
                     IKAITTHTLSLPNSLDIDKINDIKAKLANGYYDNPNAELIEKTADKIADSFLGM"
     gene            10457..11623
                     /locus_tag="PLF66_18410"
     CDS             10457..11623
                     /locus_tag="PLF66_18410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004429652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HMZ66069.1"
                     /translation="MPVLQDWVSFHFPSKLHIEIDCSAKAGHFIKNIGSRFLLITTQR
                     EMQNPNELYILKSSIEKETEGLIIYDDIENVGTFKELDTAAHFARQSQVNCIIAYGAY
                     ESVNIAKVVSILATNDFFAEELVLQTKKPKRKPLPLIVIPTMPLMGIECAPFCTILDD
                     RENERKYFSTSDLFPDLILADPKIGLHMTSSDIAKTGTSTLAAAIDTILSKYANEITN
                     SSSLRAIELVSKNIIPSIRDPKNIPAKNALYAASLLTGISQSVSSLGLCFAISLATMS
                     LTKLDIFQAMSILLPHIMEYNLTTSANKYVMIARALDEDTSDISVIEAAIKAVEGIRK
                     IYIELKIPQRLSEYEVKKNELPKIASLAYSMTFLDCLPRELPKNEIETILVAAY"
     gene            11693..12013
                     /locus_tag="PLF66_18415"
     CDS             11693..12013
                     /locus_tag="PLF66_18415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010574286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HMZ66070.1"
                     /translation="MSEEKADTIIGEDIIFKGTLRFNNSLRLKGQMKGTIESNGSLIV
                     DETGQVDADITVSSLQVNGSLRGNVEAKEKVEVGKTGTVIGDIKTPLISIETGAKFSG
                     NCLM"
     gene            12018..13829
                     /gene="recJ"
                     /locus_tag="PLF66_18420"
     CDS             12018..13829
                     /gene="recJ"
                     /locus_tag="PLF66_18420"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HMZ66071.1"
                     /translation="MPEKFIHGIDYKELTIAANNSLSPLQSHILKTILPDSKNWKEFL
                     NPDIKYLPSPFLLPDLTASIQLINRHIENKSHILLFGDRDTDGISSTTQLGIFFRTVH
                     EANGGKLTVKTSSANDDYGLCPNVLTFIKSANPNLLITLDFGTSNYEEINELASLGID
                     VIVLDHHEIPVKIPKCKLINPKREDSIYPEKKICTSTLAMKLIFAFELDQIFKKEGLP
                     SDTLFPDLESIYRNKNIEDYFEKYPTLIEKLKSLLDLSSIGTITDMMPLFGENRIVVR
                     NGIESLSKVLKTKPSNRKGLYSLMSSIKINPEKITSKDLGWGIGPALNAAGRMGKTEV
                     AVKLLFANEEKEAEIATRDLLQLNTERKERTKRNLYKVEEYFKRKPERTKEKIIFCYE
                     PDMEPGVSGIVATKLVETYKRPAIFITPDHGHGRGSIRSYQSENVLDLLNLVSDLLIH
                     FGGHKEAGGFSIELKNIPLLEQKLKTVANEWLTNQEDIITEEQSIVSFQPSDITEKIF
                     NDLQIFQPFGQENPEPLLSIKGAKIISFKPMSDGQHARFQVLGASPKIKFIIWNRASE
                     FENFISKNSTVDLFGILEENFYNNSTTIQFVVSEFQS"
     gene            13826..14764
                     /locus_tag="PLF66_18425"
     CDS             13826..14764
                     /locus_tag="PLF66_18425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017850748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HMZ66072.1"
                     /translation="MSLFNFFFPEKIEYDGKNFALVYSSYYDIYLGDHVFPGNKFSKI
                     YELICLDEHFSNISIHEPMRATREDLELVHTKEYLDDLLNLKITDRTKYSELPLNDRI
                     LDSFLHGVGGTLLATNLTETYQFVFNLGGGLHHSFSDHAEGFCYLNDVAIAATNYLEK
                     NSGKQVLIIDLDVHQGNGTAHIFKENKNVFTFSMHQDNLYPKKEVSDFDIPLEENITD
                     SVYLKLLDESLEKISKVCKPDIIYYLAGADPYEGDRLGNIKLTFRGLKERDEKIRKFA
                     VTNNSKVVILAAGGYARNFMDTVKIHYQTAQIFARK"
     gene            14766..15575
                     /locus_tag="PLF66_18430"
     CDS             14766..15575
                     /locus_tag="PLF66_18430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002745655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66073.1"
                     /translation="MNIFGDSDKRNGQGLENLSNLDLLDFSNELSTSLNLEKFIFGKF
                     SHDDIFKMLEEAKILKKIRDRGYNDFKVITYPISDLDNRIYVKSPSDEILIHIRLKVS
                     DFFVKAIQENRKMMYIDWLLTQNIKLGKLKDKKILFQGQEYPGLNIFNELTQFIFILF
                     SKMNCYGVFNIPEYFHDAVLFQKNFLFVDPIKQGHFKSILASFKNHTVRDLSLLIHSE
                     KLAFVESNEVYKWKHGEMLFTQDEYLKEVVFDRNYFEKVKHAAETRFFLLE"
     gene            15579..16685
                     /locus_tag="PLF66_18435"
     CDS             15579..16685
                     /locus_tag="PLF66_18435"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HMZ66074.1"
                     /translation="MKIEILIIDDSHEIGEALKLIVEDLGYNSKFFDSSEKALPYFQN
                     ELNPIIFLDIFLPNDNGLDLLPAIKNISPFTQVIMMSGESDINSVVSSLKNKASDFLI
                     KPFSVESVKLAVERGIEYHKLLKDNHNYQENLEQDMKFLSSIQKQVISPKIDSDTIYA
                     DFHAYSFVSGGFYHLDENENFTRIVFGEIEGNGVTSSFIGLLTLNLLKDIFQTEQSPD
                     RVLSKLNDELYFKINIHTLTAVCIVIDKIKKEIHYSNGGNAIPIVLEKNTERLKYLFT
                     DASNIIGIMPKTEFLTHTFHYSEESLLFLYNSGFIHSINSSQREFEAFLKNIQAKYSE
                     NNSFNEIKNLVDSHIAISLTKNPKQNLSFFIYKI"
     gene            complement(16659..18275)
                     /locus_tag="PLF66_18440"
     CDS             complement(16659..18275)
                     /locus_tag="PLF66_18440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66075.1"
                     /translation="MVLKNIYFIISLSLIGLLTLLPSAWFIYFDKNFFYFLILSLNFF
                     YIYRVFIKKESFFSVSLSIENRLIILLLLISFLLMVFLPIQNLNLGDGVILLEHVALE
                     AKIFGFHLTMDEILEALIHSVIYAKFQNYFNTPMEVYRIISTIAGFLGILLIYYYFKK
                     FKNSFLSYLLVFTSGGIYLFHGYSENYTLVTLMIWFYILFSIDTIRSNTHRNMKALIP
                     ICIIASFLILFHLVSGYLIFSLIYLCYYFSEDKKFIRNAIFSTVFSLVIILPVFIYFI
                     FYANVRFDFSQTHLTNPKFYPISKIISTVHLRDILFCLFGSCLLPFLLIIYALIFDKD
                     RLKKLLKLAEFKFLFFVFIGFMIHGIVHYPQLGFPADWDLLAFFWSPILFISVYIWEE
                     LYTEKMESKRTYATQFIGFFVFGISVFLLNAISLNQPSKEKYKELELSLNRIEQYSNS
                     KERETINVIEPANKKFYLSVSFFLFESKVKILNMPISDKSKLLIDENQKFSIELEEHK
                     EKIESKWQKDFYKRLTKYHLDYLDFINKKG"
     gene            complement(18319..20049)
                     /locus_tag="PLF66_18445"
     CDS             complement(18319..20049)
                     /locus_tag="PLF66_18445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66076.1"
                     /translation="MNANTGQSSGGHSAIRLGNLIYHFQFFPDKIFHIVREEWNSFRF
                     VYGIQENRTIRILPIQVSSETYNYILERMNFLYLYQQKELENLVKLKNDELIIESVVK
                     GDGLINLKGLGYFSNHNQEKENYRLLISKIENQYGSKYILENLILIRKQIYSFRFPET
                     ETIQSFDNHKTLPVVKNFSDEYLDLLSRLKAFEILYENKSLNQESLIQYEPSLSEDEK
                     LNAFKNLEKIKNQYENDIISLIRNTENSGYVLLIKIARYLTILKSLRENKFYFLDSFG
                     KNHSLLSLKVDKDKNLLYTKLREMNQRIDSTFKSRFAEKELKNLSEIEYTQIEDLLSR
                     KNEFLEGILTTKPIRIRYEIMLPTKEESIYIYKDENQKINLEELLLNYKRNAFNYERQ
                     LQLKYPFHIISQNCTTEIFHSLNSFYKNKEDVTNSLGGYIDKKDSFVFIPFYAYTKVK
                     DSYRVNDGIEVPSFRTYILSKKDTSNLLLKIKESNTLTSRYYKFNKEDSLFLFFTDDS
                     IWIRPLFGFINLIFGVGEFSFGILTFPLDSGEKLIKGLQGIFFSLPEMIFFNIRKGTF
                     LYETNFIENN"
     gene            complement(20127..20600)
                     /locus_tag="PLF66_18450"
     CDS             complement(20127..20600)
                     /locus_tag="PLF66_18450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66077.1"
                     /translation="MSLETFKFVSEIISRIELDFLFRLGLRKIFILIPIAMDGLILRL
                     KILSYNKNRIEFTVNVTMKFFLIRKLLMSFVGVAIFLIVSCGNIESEKSYCERKAEEY
                     FLLCLVDIKAAPSYYGFRDWATPQAQAYAQNYCILEYQRKKRCPDQKSYKPHRKD"
     gene            20581..>20824
                     /locus_tag="PLF66_18455"
     CDS             20581..>20824
                     /locus_tag="PLF66_18455"
                     /inference="COORDINATES: protein motif:HMM:NF012702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66078.1"
                     /translation="MNVSKLIHPIFLQDGLKQKTEIKSMPDFYRYSSDLALKDIEESL
                     LLGIHHFILFPQIEEKLKDSKASLSYKKNNLYLKFIQ"
BASE COUNT         7304 a         3392 c         3255 g         6873 t
ORIGIN      
        1 taattgaact tcttttttta taccatcaat tatctaaaaa atctcttatt attcgaaaga
       61 aacctagctt aaaaagtcac caaagattct acaaaactct ttgtatttct aaagcttttc
      121 ctgaaactcg ttttacccct ttctatgaaa aatttcccag ttcatatacc cagtgaaaga
      181 gattaaaatt ggatagactc tacaggtttt cctgaaattt tggaagggtg aatcctatcc
      241 ctgaattact tatgccggca ggcaatttag aaaaaatgaa gctcgcctat gaatatggcg
      301 cagatgcaat ttattgcgga gtacctcgtt attcactccg tgcaagagaa aatgatttta
      361 caatggaaga tttaaaatcc gctgttttgt atgcgcactc tagaaataag aaaatttatt
      421 ttacaatcaa tgcatttcca agaaattcaa aactgcctag ctatccaaaa tatttacaac
      481 aaatggctga attaaaacca gatggtttta tcatggccga tcccggttta attttcctaa
      541 ctagaaaact agctcccgaa atcaatattc atatttctgt gcaagcaaat acaatgaact
      601 atgcttctgt tgaattttgg aagtcagtgg gggcaacgcg agttatcctc tctagagaag
      661 tgagtatttc tgaagtaaag gaaattagag aaaaggttcc tgatatggaa ctagaagttt
      721 ttgttcatgg tgcggtttgt attgcacaca gtggacgatg tcttatgagt aattatttta
      781 cacaaagaga cgcaaatcaa ggtgcatgca ataacgcttg ccgtgataaa tataaagttt
      841 ttgtaacaaa taccagacaa aacgatgaac cgatggagat tattgaatct gatgaaggca
      901 cttttctaat gaactcaaaa gatatgagag caattcaata tctgaaagaa ataagagatg
      961 caggagttga ttcaattaaa gtagaaggca gaacaaagaa tgaatactat gttgcgttag
     1021 ttgctagaag ttatagaaaa gctctagatg acttgaaaga cggaaaagaa tttgatgaaa
     1081 gattgcttga tgaattaaat aaagttgctt cccgaaagta tttttctgga tttttgacaa
     1141 gaggtatgga agaaagagtt ccagttgagg agcaaaactt tcaaaattat gaaataggat
     1201 atagctatga acagagtcaa atgtatgccg gaacaattca aaaagtttta gatgattccc
     1261 tagctagttt tcagataaaa aacaaaatcg ctcttggaga aaaaatggaa gttttttttc
     1321 ctagaaattt tgaatcgaaa attttctcca tcgaagaaat gtatttcaaa ggggaaaaaa
     1381 aagaagtttt aagcggtggt atgggagaag tgcaaatcaa aatccctttt cccgtagagg
     1441 aacattgtat attaagcgtt attcgaaagt ccgagagaaa aaaagatgaa tcaaatcaaa
     1501 aagttactat ttctattcct tagttataat ttaattgtat tattaatact aggactacta
     1561 aattattctt ctggaaaact tctatccctg ccctattctg ggattcttgg aagttctaga
     1621 tggattggaa tgctatttgg aatattcttt ataaattcca ttttctcttt aggtcttatt
     1681 tttgttttac agaaaatgat cactaaagag tctttccatg aaaacatcgt tagaaactat
     1741 cttattttaa cgttagttct aatgctctgg gcaacttttc gattaagcaa tactgtttgt
     1801 atagatggca actgtaaatc tggcaccgga aggttagcct ataaaacagg ggaaatttat
     1861 gaaggggata tcgatgatta ccaagccaat ggaattggta aaatttttta tccaaacggg
     1921 gatatttatt ccggtgagat gcaggaagga aagccgaatg ggaaaggctt attgacccta
     1981 aacaatcaaa atacaaaggg aagtattgaa ggtatatggt ttaagggaaa actcaatgga
     2041 gaagcaaaaa gaattttaga accagaaaat aaaatcgaaa ttttattgta tagagatgga
     2101 atcatcagcc ataaataaaa atataaaact agtttggaga ctattatgaa aacaaatact
     2161 ttatttaaat tcttactttt atcctttatt gcgatagcaa tttattccca gaataatgat
     2221 aaacctagtt cattcttttg gacagaatca caaggcaaaa tgacattatc cgcagcaaaa
     2281 actaaatgtg ccagtctggg tctgcgtctt ccaactagag atgagatcat atcagctcat
     2341 tcaaaaggag agactaagac ttggaaaaaa gatggggagc tctattggac tctagatggt
     2401 tattctgaag aatatggata tagttttgat attacaaaag gtagctacgg atactacgaa
     2461 ggaaaactgg aaagaaccgt taggtgcatt acctcagaaa ataacaaagt agaagtaacc
     2521 acagctaaga atattcaaaa aacaaaatgg agtgattact taggtttgat gacttgggaa
     2581 gatgcaaaga aaaaatgcga atctctcgga ttaagacttc caacaaaaga ggaaatgatt
     2641 caagcctacc aaaatgggga aacgaaatct tgggaaaaag aaggcgaggc ttattggaca
     2701 tctacagaat tttccaaagg cacaaactct tattattatt ttggtgttgt gaatggattt
     2761 tcaagccatt atggtggaaa agacgatgat ggtcatgtca gatgtattca ataaattaac
     2821 aaccagttgt cagatcaaat taccagagaa tcactttctc ttatttttag aacaatttga
     2881 gtaactaacg ttattttaag ttttcatgtt tcatatttgg atttgcaaga agtgtttctg
     2941 ctctttcaat atagtaacta gaagctttat catttggata aagcttcagc actttatgaa
     3001 acaattctat agaatagcga taattcccat taaaaaaact ttgcacagct ttttcaaaat
     3061 cagttttggt ttccttaaag ttttttaatt caatttcatc aaagggagtt tttatctcaa
     3121 agacagatat ctcttctgat ttacctttta cggtaatttg tcctaaatat ctataatgaa
     3181 tcctttctaa gaacgatagc tgctttagcg ttgagtctgt gattataata tcagatccaa
     3241 atattttagt ttggctttca agacgcgatg caagatttac agtgtctcca ataacagtat
     3301 agtctaaacg ttcgtcagat ccaatattgc cagcgattgc aggacctgta tgtatcccta
     3361 ttcccatttt caaattgtat ttccattgtt tatttatctc agaaagtgat agaaacatat
     3421 caattgcggc tttcacagct tcttctgcat gctttgtatt ttcatttgga gcattccaaa
     3481 atgccataac cgcatctcca ataaatttat ctaatgttcc gtttcttttt aaaatagact
     3541 ttgccatact tcctaagtat ctatttaaaa tttgaaccac ctcttctgga cttaactgtt
     3601 ctgaaagaga tgtaaagttt ctaagatctg aaaagataac agtaatttcc cttctctgcc
     3661 cttctcctaa tttaacaccc cttttggaaa gcgcatcttc tacaacatct ttagatacat
     3721 atttatctaa aatatttcta gtctctttcc ttctttctct ttcttctaat aataatacca
     3781 agccatacac aatactaaat gaagggagat agataattcc aaattgtata ggaagattgc
     3841 tattcattct aaaaagaaat atatgaagat aaaataggag caataaagta gatcctaaga
     3901 aaataacctt ttttaataac gaatagtggt gataaaaaat ataacaggat atagaactaa
     3961 ggattaaata gatgcaaatt tccaactcaa atggaagatc atttctaaaa tcgcctaaaa
     4021 gaaagttaga cgctacaatt gcttgaatta ctgtccctgg taattcccca tatggggagt
     4081 tatgtgtatc acttgcaccc gaggaagttg ccccaattag aacaatttta tctttaaaga
     4141 tatctggtaa aataagattc tcttcttttt caattccctc accagaaccc attttataaa
     4201 tagaataaaa aatatcagat accgatatgc gaatgagagt atctaaagat ttctttgaat
     4261 agtaatgtat tggaacaaac ccattttgat gagaaagttt tattttatga tttttgattt
     4321 gatatataat ttcttctttt tttatggaga gttttaagtt ttcctcaaaa aaaatcaatg
     4381 ctaaccaagc aagggatgga tatgtatgat tagctgccgc atatccaaaa ttataatttc
     4441 taataactcc atcaggatct gatttaaaat taaccccatg catatgactg gaaacatttc
     4501 gaatagactc aaccggaaat gctccttcta aagaaaaatt ttgagtcaac aaaggattac
     4561 cagaaaccac tgcaaactta ctaatctcaa ctgaattttt ttctgttttt tcttccaact
     4621 gattaaaata aaaagcatgc gatagatttt tatttctttt tgtgatattg actaagtatt
     4681 tatcattttc tgttatttcc gaaaacaaaa tatcaaataa aatccctttt ggtgatcctg
     4741 atgagatata atctatgaga ggttcataca catttcgttt ccaaggccaa cgcccaaaag
     4801 aagggctatc gcctaactca tataaagttt tatcatctat ttcaattaat actatatctt
     4861 tgctgataag attttctctt tcaatataat tgtattttac atctatgatt ttatttgaaa
     4921 ggtcagctat tatggctaac ttataaaaaa taaaactaaa taaaaatgga atgaaaagaa
     4981 aacagattgc atatagataa atgtttccta agaagttaac gttttctaaa tgcttatttt
     5041 ttaaaatttg catttaattt ctcccagtcc tcttttccta tacatgaatt tgttttccta
     5101 gatttatttt taaattctct ttgtatcact tgcactctat ctttaaaact gggatgtgtt
     5161 ttttgaatcc aatctgttct cttcgtcaat ttctccattt tttctaaata agatagtgca
     5221 gacaatggat tacatcctaa ttctattaaa atatatcctg cactaatatc tgattcaaat
     5281 tcttgagctg gttctctacc ggtttcttgc aacatagtat cccatttttt ttcaaattca
     5341 tttgctgcaa cggaaattcc gcctgttgga tctagccctg cctgtaaaaa cttttcaaaa
     5401 gaattattat tttcaggctg gaaagaatgg gagaggctaa tatgtgttaa ttcatgggct
     5461 aatacaaact cagcactttc gattgaatcc catgttttaa tcatcccgcg agaaatgagg
     5521 atatatcctc caggaataga gaaagcaaaa acatcatcag tatcaaggat tcctgctcgg
     5581 taatataaat cgtttcttga agtaaaagct gcaacttttc gaaccagact atttaaagca
     5641 attgttgact ccctgtcatg caatagatga tatctgatta aaaaattctt tactatctgt
     5701 cttccaaata acgtctcctg tttgactctt tccgattctt gtgtcttatt ttcaacggaa
     5761 gaacatgtat aaattaaaaa tattataaaa aatataactc tcattcactc attgccgtta
     5821 tcgaaaaatt aacttgatcc tgcaaaaaca aaactggtgt agaaggagct tggaaataat
     5881 aaacagatcc acctcttccc ctttggtgga tcactgtaat ggaaacagat gtattcctag
     5941 aaatatagta agaaaagcca agcgttattc cccttgatga tacatcattt tgataatatg
     6001 catcattaaa atcatactgg taatggccac ctctaatacc agcatagtat atcgtatttg
     6061 ctggatcgat tgctatacga ttcgatgcgt attcatagat actcgactct ataacagatt
     6121 gaggccatct aatcttttta ttggatgaat gatttgtaaa ataacctata cgaattgcaa
     6181 atacttgatt cacaaagtat tctaaaccag tggcatagtt tttggtttcg gttcttctta
     6241 gttcatgata ttcatcatcg ccaaaagtga ttctaagagg attactaaaa tttacaatgt
     6301 agttttgtcc ggataattta taaggtgagg tatagataaa atctcccacc cataataatt
     6361 tttccgaaaa ataataggac agtccggttc tgatttctgt gggagcaggc attgatacat
     6421 ttgatttcgt ttggataaat ggtgaatctg tatagtcaac cccaccacta ccaaaaccag
     6481 atttatagat aaaataattt tcatctgaat agtaagaatt tcgtctacct gcagtgttta
     6541 tgattttacg agttgaaaat cctaaatata atttgtcggt taaagcatat acgaatccaa
     6601 gaattggttc aaaacctatc gataactttc tatcgtagta aatctctctc attcttgtct
     6661 tatcaaactt ctcatactca tcggtcgcaa aaagaacgtg agtctctctc acataataca
     6721 atgttgctcc aatcgaaaga cgatccgtta agcgatatgc tagactaaga ccaaattgtg
     6781 ttcgaattaa ttcttcttta ttgattgata cataattgct ataggcgtat tgcccctcat
     6841 atagctgaaa tggtagagta atggaattgg caacatccgt ttgtgactga taaggactta
     6901 caaatgaaaa tccatatttt aatttttctc ctcctccgac atacccgaga aaattgggaa
     6961 aataggaact tgattttcga atataatcac gacctgttcc atctgcattt gtataattat
     7021 cataaccttt ttgataacca gaactactcg ctgtaaaaga acttttattc gcgaagccta
     7081 gccctcctgg attgtagtaa gtgccagtag cgtcatccga tacagctgta taagctccac
     7141 ccattccact ggctctctcc cctaagaaac tatgaatatt ttgaaatact tgatcagcct
     7201 ctacttcctg aatagaaaaa atcaaaaata aaattattcg gaagtagttt tttctaacca
     7261 acgaagacta ctctcagctt ctctctctga ttggataatt gaagtagaat ctttatcaga
     7321 gtataaaaat cctctagacg ctaatgttcc tgtaatgtca ttcaaacgcg ctctagatct
     7381 tgtgatagat tgacgtttaa aatcggactc acttaacttt aaaggttttt ctgcggaaga
     7441 tacaggagat gtgtgaattt ttagaatcca accttggata ccagaacaat ctacaaatac
     7501 tttcccagat tcttccttta aaatcttccc tctgcaatct gcttgaaccg atttgattcc
     7561 ggtctgtaat gagggatctt ggtataattt aggttcctta ccggaaatgt atacccaatc
     7621 atttgcacct agagggagaa gattgaatat acttaataat aaaaagaatt gaattagttt
     7681 tttcattgaa ttcgcctact aaaatcctta tatatttcaa gatttaatat tacaaataat
     7741 tttttttaaa tcttgaaata tctagaattt attacgacta gctaaagctt ctgaaaatag
     7801 tagattttat tgttaaattc ataaaaagaa aaacgttaat aacaagaatc taactttttt
     7861 aagggtgatc aaccgcctaa ctttttagca agatcctctc tttgggcttc caattgtttc
     7921 atttggtctt tagttccatc cagaatcttt tgaaattgcc catctttatc tacttctcca
     7981 tcttcttttt gtgcttcaat taaatactcg ataatttgaa gtctatcttc tataaccttt
     8041 ttttgatgat taaaatgtgt atctagatca tctcttgtat aggttccact gttaacagca
     8101 ttctgtgctt tattccatct ctcctctaac tgggcttgct ttgctttgtc ttctctagac
     8161 atacgtctcg gaattaaatc gttatccgca aagagttttc gaagttcatc aaattgttgc
     8221 attgcatctt cttcccaagg cttacctgtt tgtggattga tcggtatctc accttgttcc
     8281 ccattcgcgg ctgtggattc ctcttcttga taagaaattc ctgacttcat aaaatcactt
     8341 tcaaacagag atgaattatt atttcttcgc cctctccttt tttcggaagc gctctctcca
     8401 tcttcactac ctctcccacc tccccctaaa agaaggctta atttcttaga ttcaacacgc
     8461 tttttctttt tttcacctga aaaaaaagcg aaatacccga ctatagagac tattaaaata
     8521 gagattaata caatggattt attatttgtc atagaattag tttcttacaa ttcaattcta
     8581 ttgaaaacaa aaaaactagt ttttttgttt ggcaattcta tcgaatttga aaaatggaag
     8641 taaaatgctt aagtttataa aaaaactcat cctccttctc ggaattccga ccgtttcttt
     8701 ttgcggaacg aatactgata ctcctgtggt tttggcacca cttatttttt taactagtcc
     8761 tatcaatgtt ccacaactcg taggtgtaat tccgacacaa gagtacccaa cgtcaggaac
     8821 aggactatca gatttaggag gttatgtaaa tccaaatgcg cctcagttta tcattaaata
     8881 cttcgtaaca aatacagaac agcagttcat aggttataat ctatctatta cttctcaaac
     8941 tcctacactc atagagacac aagctggaac agccggttcg gtttatacag aaaatggaat
     9001 tcagccctca tttcctcatt tagccactga aaactcgacg gcaaaggaga atatgaagcg
     9061 tagaagaatt gcttggagag ttccccctcc tggaattgtt tatttccaaa aatgtgaaat
     9121 gtataatttt acacttcgag cagtgcttag caatgttcct caaacctcta atccttctgc
     9181 cgctgtttct gcttgcgcgg gaattgaccc aagcaaatgc ccagttggaa gtagctgtaa
     9241 tccgagttct tgcacagaca ctcagtgtag tttgacaata agagctacct gtccggttgg
     9301 gactctttgc aatccatgca ctatattaga ttctggtggt gtagcgaaag ctggatgtga
     9361 atgtccagat ggagtctccc ctaatttata tgcgagtcca atcgtatcct gcaatcgcta
     9421 atgaaaaatg gatccctcta catcgtagga actcctatcg gtaatttaga agatatcact
     9481 ctaagagcga ttaaaacact aaatcaagta gatttaatac tttgtgaaaa tgcaaagaat
     9541 tcacaaaaac ttctaaatca tcatcaaata aaaacaccct ataaaactct ttttactaat
     9601 acggattcag cctatgattg gattatagag gatttaaagt ccggcaagga aatggcatat
     9661 atatcagacg caggaacacc aggagtttca gatccaggag ctggtttggt tcgatcagtt
     9721 agaaaagcag ggatttcaat agttcctatc cctggagcaa gtgctttaac aagtatgctc
     9781 tctatttcag gataccaaac aaatcccaca tattttctag gctttctatc tgaaaaaccg
     9841 aataaaaaaa gaacagaact ggcagagtat acagaaaagg attgtgtcat tgtattctat
     9901 gaatctgtgt ataaaatcaa aacaacactt tcaattctac aagagctatt tccggattca
     9961 gaaatcttga ttgggcgcga attaactaaa atacacgaag agattctttt atataaacca
    10021 gaggatttac ctatagaatc cattacatta aaaggagaat ttgttctatt aatcaacaac
    10081 catagaaaaa aaatcgctaa agagaaaata atcttaaccg atacgtaata aggagagagg
    10141 tagcatgtta tgtttataga taaaattaaa aatattggtt cggcatttga gcctaagaaa
    10201 tctgcttctg tcaaaaaaag ttcagagata ctcccaagtg acaatgtgca gatatccgat
    10261 gctgcaaaac taaaagcaga tataaaggcg atcaccaccc acacgttatc tcttccgaat
    10321 tctctggaca ttgataagat aaatgatatc aaagcgaaat tagctaatgg atattatgac
    10381 aatccaaacg ccgaactaat cgaaaaaact gcagataaaa ttgcagattc atttcttgga
    10441 atgtaaataa gtttaaatgc ctgttttaca agactgggtc agttttcatt tccctagtaa
    10501 gctccatatt gaaatagatt gctctgccaa ggcagggcat tttattaaaa atattggttc
    10561 tagattttta ttgattacta cccaaagaga aatgcaaaat ccaaacgagt tgtatattct
    10621 aaagtctagt attgaaaaag aaacagaagg tttaatcatt tatgatgaca tagagaatgt
    10681 tggaaccttt aaagaattgg atacagcagc tcactttgca aggcagtcac aagtaaattg
    10741 tatcattgcc tacggtgctt atgaatccgt aaatattgca aaggttgttt cgattcttgc
    10801 aacgaacgat ttctttgcag aagaacttgt tttgcaaact aaaaagccca aaagaaaacc
    10861 tttaccgttg attgtaattc ctacaatgcc ccttatggga attgaatgtg cgcctttttg
    10921 cactattcta gatgatagag aaaacgagag aaaatacttc tctacatcgg acttatttcc
    10981 tgatctgatt ctagcagacc ctaaaattgg actacatatg acatcgtcag atattgccaa
    11041 aacgggaact tcaactttag cagcagcgat tgatacaatt ctatccaagt atgcaaatga
    11101 gattacaaat tcttcctctt taagagccat agaacttgtc tccaagaata ttataccatc
    11161 gattcgagac cccaaaaata ttcctgcaaa gaatgcgcta tatgctgcta gtttattaac
    11221 tggaattagc caatctgtta gctccttagg gctatgtttt gcaatctccc ttgcaaccat
    11281 gagtctcaca aaattagata ttttccaagc catgtccatt ttgttacctc atattatgga
    11341 atataattta acaacctcag cgaataaata tgttatgatc gcaagagctc tagatgaaga
    11401 tacgagcgat atttccgtaa ttgaagcagc tattaaagcc gttgaaggta ttcgaaaaat
    11461 ttacattgaa ctcaaaattc cacaaagact ttcagaatat gaagtcaaaa aaaatgaatt
    11521 gcctaagatt gcgtctcttg cctattcaat gactttttta gattgtcttc ctagagaact
    11581 tccaaaaaac gaaatagaaa ccattctagt tgcagcttac taattttttt tcttgcatcg
    11641 aattccctct catagttgtc tgtattgata tttaaaaaaa gcaggcttat atatgagtga
    11701 agaaaaagca gatacaatta ttggagagga cattattttc aagggaactc ttcgatttaa
    11761 taactcattg cgattaaagg gacaaatgaa agggaccatc gaatcaaatg gtagccttat
    11821 cgttgatgaa accggtcaag ttgatgcaga tattactgta agttctcttc aagtgaatgg
    11881 atctcttcgt ggaaatgttg aagcaaaaga gaaagtcgaa gttggtaaaa caggaacagt
    11941 tataggtgat attaagactc cattaattag catcgaaact ggggcaaaat tttctggaaa
    12001 ttgtctaatg taatatcatg ccagagaaat ttatacatgg cattgattat aaagaattaa
    12061 caattgcggc aaacaatagt ttgtcgccac ttcaatcaca catattaaaa acaattcttc
    12121 ctgattcaaa aaactggaaa gagtttttaa atcctgatat taaatatctc ccttctccct
    12181 ttcttttgcc tgatctgact gcctcaatcc aattaataaa taggcatatt gaaaacaaaa
    12241 gtcatattct tttgtttggg gatagagata ctgatggaat ttcctcaaca acgcaactag
    12301 gcattttttt tagaactgtt catgaagcaa atggtggaaa acttactgtc aagacatcat
    12361 ccgctaatga tgattatggt ctatgtccta acgttcttac ttttattaaa tctgcaaatc
    12421 ccaatctttt aatcacatta gattttggaa catctaatta tgaagagata aatgaattag
    12481 catctcttgg tattgatgta atcgttttgg atcaccatga aattccagtt aaaattccaa
    12541 aatgcaaatt aattaaccct aaaagagagg attcgattta ccctgagaag aaaatctgta
    12601 cttctactct agctatgaaa ttaatttttg cctttgagct agatcaaatt tttaaaaagg
    12661 aaggactacc ttctgatact ttatttcctg atctagaaag catttataga aataaaaaca
    12721 ttgaagatta ttttgaaaag tatccaactc taattgagaa gttgaaatcg ttattagatt
    12781 tatcatccat tggaactata acagacatga tgcctctatt tggtgaaaat agaatcgttg
    12841 ttcgaaatgg catagaatct ctatctaaag ttttgaagac aaagcctagt aatcgaaagg
    12901 gactttactc ccttatgagt tccatcaaaa ttaatccgga aaaaataaca tctaaagatt
    12961 taggctgggg aattggtcct gccttaaatg ctgctggtcg aatgggcaag acagaagtag
    13021 cggtaaaatt attgtttgca aatgaagaaa aagaagcaga aatagctact cgagatttac
    13081 ttcaattgaa cacagagaga aaagaaagaa caaaacgaaa tctttacaaa gttgaagaat
    13141 attttaaacg aaaaccagaa agaacgaaag aaaaaattat tttttgctat gagccagaca
    13201 tggaacctgg agtatcaggt atcgtagcaa caaagttagt cgaaacatat aagcggcctg
    13261 caatttttat tactcctgat catggacatg ggcgaggaag tataaggtca taccaatctg
    13321 aaaatgtatt ggatctttta aatttagttt ctgatttact cattcacttc ggtggtcata
    13381 aagaagctgg tggcttttca attgaattaa agaatatccc attattagaa caaaagctaa
    13441 agactgtagc aaacgaatgg ctaacaaatc aagaagatat tataactgag gagcaaagca
    13501 tagttagctt tcagccttca gatattaccg aaaaaatttt taatgatctg caaatctttc
    13561 agccctttgg tcaagaaaat ccagaaccct tactatcaat aaaaggcgct aagataatta
    13621 gttttaaacc aatgtcagat ggacaacatg ctcgttttca agttctagga gcgagtccaa
    13681 aaataaaatt tatcatttgg aatagagcca gtgaatttga aaattttatt tcaaaaaatt
    13741 caactgttga tctatttggt atactcgaag aaaattttta taataatagt acgacaatac
    13801 aatttgttgt atcagaattt cagtcatgag tctttttaat ttttttttcc ctgagaaaat
    13861 agaatacgat ggaaaaaact ttgctctcgt ttattcttct tactatgata tttatttagg
    13921 cgatcatgtc tttccaggaa ataagttctc caaaatctat gaattgattt gtttggatga
    13981 gcattttagt aatatctcaa ttcatgaacc aatgagggca acgcgggaag atttagaatt
    14041 agtccacaca aaagaatatt tagatgatct attaaatctt aagattacag atcgaaccaa
    14101 atattctgaa ctacccttaa atgacagaat tttagatagt tttttacatg gcgttggagg
    14161 cacactcctc gctactaatc taaccgagac gtatcaattt gtttttaatc taggcggagg
    14221 tctgcatcat agcttttctg atcatgcaga aggattctgt tatctaaatg atgtagccat
    14281 tgctgccacc aattatttag aaaaaaatag cggcaaacag gtattaatca tagacttaga
    14341 tgtacatcaa ggaaacggaa cggctcatat ctttaaagaa aataagaatg ttttcacatt
    14401 ttctatgcat caggacaatc tttatcctaa aaaagaggta tcagattttg atattcctct
    14461 agaagaaaac atcacagatt ctgtttactt gaaactccta gatgaatctt tagaaaaaat
    14521 aagtaaggta tgtaaacctg atataattta ttacctagca ggtgcagatc catatgaggg
    14581 ggatcgttta ggaaacatta aattaacttt tagaggtcta aaggagagag atgaaaaaat
    14641 aagaaagttt gcagttacga ataattcaaa ggtagtgatt cttgccgctg gcggatacgc
    14701 gcgaaatttt atggatacag taaaaatcca ttatcaaact gcacaaattt ttgcgaggaa
    14761 atgaaatgaa tatctttggt gatagtgata aaagaaatgg tcaaggtctt gaaaatttat
    14821 ctaatttaga tttactagat ttttcaaatg aactaagtac atcactcaat ctggaaaaat
    14881 ttatctttgg taaattctca catgatgata tttttaaaat gcttgaggaa gccaaaattt
    14941 taaaaaaaat tagagatagg ggttataatg atttcaaagt cataacctat ccgatttctg
    15001 atttggataa tagaatttac gtaaaatctc caagtgatga aatcttaatt catattcgtt
    15061 taaaagtatc agatttcttt gtaaaagcga ttcaagagaa tcgtaaaatg atgtatatcg
    15121 attggctact cacccaaaat ataaaacttg gaaaattaaa agataagaaa atactatttc
    15181 aagggcaaga atacccagga ctaaatatat ttaacgaatt aactcaattt atctttattc
    15241 ttttttctaa aatgaactgt tatggtgtct tcaatattcc agagtatttt catgatgctg
    15301 ttctatttca aaaaaacttt ttgtttgttg acccaatcaa gcaaggtcat tttaaatcta
    15361 tactagcaag ttttaaaaat catacggttc gagatttaag cctactcatc cattcggaaa
    15421 aattagcttt tgttgaatca aatgaagtgt ataaatggaa acatggggaa atgcttttta
    15481 cacaggatga atatttaaaa gaagtagttt tcgatagaaa ctatttcgaa aaagtaaagc
    15541 atgcggcaga aacccgattt tttttattgg agtaaaaatt gaaaatagaa attttgatta
    15601 tagatgattc tcatgaaatt ggagaagctt taaaattaat agttgaagat ttaggataca
    15661 actcaaaatt ttttgattca tcagaaaaag ctttacccta ctttcaaaac gaattgaatc
    15721 cgattatttt tttggatata tttcttccaa atgacaacgg attggatcta ttacccgcaa
    15781 taaaaaatat tagtccattt acacaagtaa ttatgatgag cggagaaagt gatattaatt
    15841 ccgttgtcag tagtttaaaa aacaaagcat ctgatttctt gataaagcct ttctctgttg
    15901 aatctgtaaa acttgccgta gaaagaggaa ttgagtatca taaattacta aaagataatc
    15961 acaactatca agagaactta gaacaagata tgaaattttt atcctctatt caaaagcagg
    16021 taatctcacc aaagattgat tcagatacaa tctacgctga ttttcacgcc tactcttttg
    16081 tatctggagg attttatcat ttagatgaaa acgaaaactt tactagaatc gtttttggtg
    16141 agattgaggg taatggtgtt acttctagct ttattggtct acttacatta aaccttttaa
    16201 aagatatttt ccaaacagag caaagcccag atagagtttt atctaaatta aatgatgaat
    16261 tgtattttaa aatcaatata catacgttaa ccgctgtatg tattgtaatt gataaaataa
    16321 aaaaagaaat tcattattca aacggaggaa atgcaattcc tatcgtatta gagaaaaata
    16381 cagaaagatt gaagtattta tttacagatg catcaaacat aattggtata atgcctaaaa
    16441 ctgaatttct aacacatact tttcattatt cagaagagtc attattattt ttatacaatt
    16501 ctggatttat acactcaatc aatagttctc aacgagagtt tgaagcgttt ctaaagaaca
    16561 tacaagctaa gtattcagaa aataattcct tcaatgaaat aaaaaactta gtcgattctc
    16621 atattgccat aagtttaaca aaaaatccaa agcaaaactt atcctttttt atttataaaa
    16681 tctaaataat ctaaatgata ttttgtaagt ctcttataaa aatccttttg ccattttgat
    16741 tcaatttttt ctttgtgttc ctccaattca atactaaact tttgattttc atctatcaat
    16801 agtttagatt tgtcggaaat cggcatattt aaaattttta cttttgattc gaataaaaag
    16861 aaactaacgc ttaaatagaa ttttttattt gctggttcta taacgtttat agtctccctc
    16921 tcttttgagt ttgaatattg ttcaattcta tttagagaga gttctagttc tttatatttt
    16981 tctttcgaag gttgatttaa ggaaattgca tttaaaagaa aaaccgaaat tccaaaaaca
    17041 aaaaatccaa taaattgtgt tgcataagtt cttttgcttt ccattttttc ggtataaagt
    17101 tcttcccaga tatacacaga tataaaaagg ataggactcc aaaaaaatgc gagtagatcc
    17161 caatcagctg gaaatccaag ttgcggataa tgcacaattc catgtatcat aaatccaata
    17221 aaaacaaaaa ataaaaattt aaattctgct aatttgagta attttttcaa tctgtcttta
    17281 tcgaaaataa gtgcatagat gattaataaa aatggaagca aacaagatcc aaataaacaa
    17341 aagagaatat ctcgaagatg aactgtagaa ataatttttg aaattggata aaattttgga
    17401 tttgttaaat gcgtttgaga aaaatcaaat cgaacatttg cataaaatat aaaataaata
    17461 aacactggaa gaatgattac taatgaaaag accgtagaga atatcgcatt tcgaataaat
    17521 tttttatcct ctgaaaaata atagcataaa taaataagag aaaagattaa atatccactg
    17581 actaaatgaa aaagaatcag aaaactagct atgatacaaa taggtattaa agccttcata
    17641 ttccgatgag tattagaccg gatagtatca atggaaaaaa gaatataaaa ccatatcatt
    17701 aaagtaacta atgtatagtt ctcactgtat ccatgaaata agtaaatccc tccagatgta
    17761 aaaacaagta aataacttaa aaaactattt ttaaacttct tgaaataata atagataagt
    17821 aaaattccca agaatccagc gatagtgctt atgatcctat atacttccat cggtgtattg
    17881 aaataatttt ggaattttgc atatattaca gagtgtatta aagcttcaag aatttcatcc
    17941 atcgttaggt gaaatccaaa tatttttgcc tccaaggcaa catgttctag taatataact
    18001 ccatccccta aattaagatt ttgaattggt agaaatacca tcaataaaaa tgatattaat
    18061 aacaataaaa tgataagtcg attttcaata gagagggaga cagagaaaaa actctctttt
    18121 tttataaaga ctctatagat ataaaagaag tttaaagaaa gaataagaaa atagaaaaaa
    18181 ttcttatcaa aataaataaa ccaagcagag ggcaaaagag tgagaagccc aatcaatgac
    18241 aaactaataa taaaataaat attttttaaa accatttaac gttatcctat aatcctattc
    18301 gttttttaag gagttaattt aattattctc gataaaattt gtttcatata aaaaagttcc
    18361 ttttcgaatg ttaaaaaaaa tcatttcggg caagctaaaa aaaatccctt gtagaccttt
    18421 aatcagtttt tcacctgaat ccaaaggaaa agtcaaaatg ccaaatgaaa attccccaac
    18481 tccgaagatt aaatttataa atccaaagag aggtcttatc caaatactat catctgtaaa
    18541 aaataagaac agagaatctt ctttattaaa tttatagtat ctagatgtta gagtatttga
    18601 ttccttaatt tttaaaagta aatttgatgt atctttcttt gaaagaatat aagttctaaa
    18661 cgaaggcact tcgattccat cattcactct atatgaatct ttgactttag tgtatgcata
    18721 gaatggaata aatacaaagg agtctttttt atcaatatat ccacctaaac tatttgtaac
    18781 atcttcctta tttttataaa aagaattcag agaatgaaaa atctcagtag tgcaattttg
    18841 agaaattata tgaaaaggat attttagttg tagttgtctt tcataattga atgcatttct
    18901 cttgtagttt aataataatt cttctagatt tattttttga ttctcatctt tataaatata
    18961 aatcgattct tcttttgttg gtagcattat ctcatatcta atgcggattg gctttgtggt
    19021 aagtataccc tctagaaatt cattttttct agataacaaa tcttctattt gggtatattc
    19081 tatctcagat aaattcttta actctttctc tgcaaatcta gatttaaagg tcgagtcgat
    19141 tctctggttc atttctctca atttagtata taaaagattc ttatctttat ctacttttag
    19201 agacaaaaga gaatgatttt tcccaaaact atctaaaaaa taaaatttat tctccctcaa
    19261 actctttagg attgttaaat atctagcaat tttgattaga agcacataac cgctattttc
    19321 cgtatttctt attaatgaaa ttatatcatt ctcgtattga tttttaattt tttctaagtt
    19381 tttgaatgca tttaattttt catcctcaga aagactcggt tcatattgga ttagactttc
    19441 ttgatttaaa cttttatttt catataaaat ttcgaaagct tttagtcttg ataataaatc
    19501 taaatattca tctgaaaagt ttttaactac tggtaacgtt ttgtgattat cgaaagattg
    19561 gattgtctca gtttctggaa atcgaaaaga gtatatctgc tttcttatta aaattagatt
    19621 ttcaagaata tacttacttc catattgatt ttcaatttta gatattaata atctataatt
    19681 ttctttttct tgattatgat ttgaaaaata tcctaatcct ttcaaattga tcaacccatc
    19741 accttttact actgattcaa taataagttc atcattcttt aattttacaa ggttttccaa
    19801 ttctttctgt tgatacaaat aaagaaaatt cattcgctct aaaatataat tgtatgtttc
    19861 gctagaaact tgaattggaa ggatgcgaat cgttctattt tcttgaatac catagacaaa
    19921 tcgaaaacta ttccattctt cccgcacaat atgaaatatt ttatctggaa aaaattgaaa
    19981 atggtagatc aaatttccta aacgtattgc tgaatgcccc ccactcgatt gtcctgtatt
    20041 tgcattcaca tatataaaat ctagaaaata tgaaggttct gcaactatcg cttgaataga
    20101 agtaaaagca aatattataa atagtcttaa tcttttcgat gtggtttgta agatttttga
    20161 tctggacaac gtttctttct ctgatattcg agtatgcaat aattttgcgc ataggcttga
    20221 gcttgtggag ttgcccaatc tctaaatcca tagtaggaag gtgcggcttt tatatccact
    20281 aagcacagca aaaaatattc ttctgctttt ctctcacaat atgatttttc tgattctata
    20341 ttcccgcaac ttacaataag aaaaattgcc acaccaacaa acgacattag taattttcga
    20401 atcagaaaaa atttcatagt aacatttact gtaaattcaa tcctattttt gttgtaactt
    20461 aaaattttta atcgtaaaat cagtccatcc atcgctattg gtattaatat gaatattttg
    20521 cgaagaccaa gacgaaatag aaagtctagt tcaattcgag agatgatttc ggaaacaaat
    20581 ttgaatgttt ctaaactcat tcatccgatc tttttacaag atggtttaaa acaaaaaacc
    20641 gaaattaaat caatgccaga tttttataga tactcttccg acttagcttt aaaagacatt
    20701 gaagagtcac ttttattagg tatccaccac tttattcttt ttccccagat agaagaaaaa
    20761 ttaaaagatt ctaaagctag tttgagttac aaaaaaaata acctatattt aaaattcatt
    20821 caaa
//
LOCUS       DAOQOS010000097        20765 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_1244_length_20765_cov_6.532641, whole genome shotgun sequence.
ACCESSION   DAOQOS010000097 DAOQOS010000000
VERSION     DAOQOS010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 20765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20765
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1244_length_20765_cov_6.532641"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            177..1115
                     /locus_tag="PLF66_18460"
     CDS             177..1115
                     /locus_tag="PLF66_18460"
                     /inference="COORDINATES: protein motif:HMM:NF013046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66079.1"
                     /translation="MSIYQDKINLVYRKEMVSQQKVGASRSPNKPKYLIDFLTQNGMI
                     THFTLYDKWDPFQKEDFYLAHTKQYVDAFFAGKEPLASSNGLNWNAQFADSVRYTNAS
                     LFHAIEQSILHPDTIQFSPTSGFHHASPSHGSAFCTFSGQVIASVNIWNQYKLRGAYI
                     DLDGHFGNSIEDSKGFIKGLNKIIPYNINPTNAHKVYIENLTKELNRLSLDLLSGNVD
                     YIVFCHGADSHEWDDLGHQCSTEEWLLCSELVYKMIHRLSNKLGKPIPLSLSLFGGYR
                     SDDFNSVLSLHTADLSRCLDILCGQNTQYLPIVKER"
     gene            1363..2247
                     /locus_tag="PLF66_18465"
     CDS             1363..2247
                     /locus_tag="PLF66_18465"
                     /inference="COORDINATES: protein motif:HMM:NF017051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HMZ66080.1"
                     /translation="MWSNLLILHSNDKNRDRESFHGLHHWKTCMRTLYIGDIRIFDLS
                     QAQFSRHYEAYVGYEAYKLLLEIVSGIRSRLLGETEILAQFKETLKNEALPEHSLGDY
                     LKKLRDQIIEDSRKIRSGHLRFLGDQSYGGLANRYLKNTKEVTLFGTGNLAVKVLPWL
                     LEKNRKVKVVGRNLEKLKDIASKFPVQIQTLDEYKVADEAIVIAAPISMKEWIPSFSE
                     DKIIIDFREDDKKDDFSKNKNYISFEFMLNSLKAQEERNAELRKKLDVVIQELAAERE
                     NESINFIYGWEDIPCLSH"
     gene            2232..3767
                     /locus_tag="PLF66_18470"
     CDS             2232..3767
                     /locus_tag="PLF66_18470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008588827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen synthase"
                     /protein_id="HMZ66081.1"
                     /translation="MSLSLKIGSRKSALAKLQSYLVAKALREKFPGIQIEFYFKESLG
                     DKDLTSPLWKMGDRGVFTKDFKEDLLSETVDVVIHSWKDLDLQHEDNTEVISILERAD
                     QRDLLLFKREHLLNPNFDEIKIFSSSPRREYNLKRFLSKALPKRLHGKPILFEPVRGN
                     MQTRLAKWKQADTVQGLILAKAALDRLLATNFPESESDEYSEIRKTIRAYLKESVFMC
                     LPLSENPNAPAQGALAAEIKASRSDIREIISKLTIHSVSRSVNIERQELQKYGGGCHQ
                     KIGVSAIAKNYGEVFYLRGLTDSNVELNSHKILKENPSQPKAKRMTDIFPNEKEKFKF
                     KRESLPEPEIPNSNILVSRTNAWHPNWSRVDLDQIIWAAGIKTMYQLAEKDLWVSGTL
                     DGLGEEESPNISMLVENNKPFVKLTHEDSSEIESEMERIYTYKIFLEEEIPDLSNKTH
                     FFWMSGYQFDLALQKYPEIIHKFHACGPGITAKHIQKKLGSNSSLEIFLNYEDWLHFH
                     TTD"
     gene            complement(3832..4746)
                     /locus_tag="PLF66_18475"
     CDS             complement(3832..4746)
                     /locus_tag="PLF66_18475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010572421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HMZ66082.1"
                     /translation="MIYQLTSLFPLWILIVSIAAFIYPNLISWFKGEYISFALGIIML
                     GMGLTLTLDDFKRILKFPKSVFIGIFLQYTVMPVSGYIVALLYDLPNPLAVGLILVAC
                     CPGGTASNVLTFIAKADVALSVTMTAISTLISVIMTPILTFYLAGNRVDVNASGLLFS
                     TLKVIILPVAIGVFLNFLFPKATKKISNISPLLAVVSIILIVASVIASNKAPIIESGI
                     RLLASVFTLHIFGFFFGYVLSVLMKERKISARTISIEVGMQNSGLGVVLARENFSNPL
                     TAVPCAISSLAHSLIASVLAAFWRRNSD"
     gene            complement(4748..6112)
                     /locus_tag="PLF66_18480"
     CDS             complement(4748..6112)
                     /locus_tag="PLF66_18480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002977843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66083.1"
                     /translation="MKKYIFTLCLFLFIGCKKEVSISMVTATSMQNGLPFLALNDSKW
                     MPEANAEFVKLHVYLDEAVQLSKVEVNSCSDDFKSQIDMYINFDEMIQTMKAQGKVAA
                     HSFESPVTARSVTFNFSKNSNICLASVHLFDEKGKQFKVKAPRIVEGTAEASETGKPE
                     ASYGVMNLFDSRYEYSYASLNNSKGVSLSFNFKDTQKIQSIKVWNGYQRSDVHCVENG
                     RVKTINLTGDEGYSEKISLKDEMGPQIVELPKPFNGKKLVMTIDESYRGKKYQGIVLS
                     ELRFFDGKEWFLLDPFPRIKSIAKTNGDDFQKAGISEILNKSLTGSEEGVNSNWTVRL
                     RSDGSMFLEGSTTTYDSDSENTSRFYALGNYEVKSTENNILQVRIFGFLREIKSEMQI
                     EHMDCNGCGRDCNTLGSGGDPNKIEKIFAEFLELEKVNDTFVVRNLKRSNNLNFNSLN
                     MSLE"
     gene            complement(6366..7178)
                     /gene="pyrF"
                     /locus_tag="PLF66_18485"
     CDS             complement(6366..7178)
                     /gene="pyrF"
                     /locus_tag="PLF66_18485"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008400661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HMZ66084.1"
                     /translation="MSFYQKIITRSNSLRSLLCIGLDPEWEKLPASIKNSENALYTFC
                     KEIVDATHDITIAYKPNIAFFERFGFKGIEQFEKLIRHITSNYPQVAIVADIKRGDLA
                     NTAKEYAKYYFGDLKVDSLTLSPYMGSDSIEPFFEYKGHAVFLLCLTSNPASKDLQKI
                     KTVNGKYFYEEVANFSKSLNSKFKDQVGLVVGATHPDELGQIRNAFPELAFLIPGYGA
                     QGGNLSDIMKVSARNSLINSSRSILFNSNESDFALKAREKAIEINREMKELF"
     gene            7372..7650
                     /locus_tag="PLF66_18490"
     CDS             7372..7650
                     /locus_tag="PLF66_18490"
                     /inference="COORDINATES: protein motif:HMM:NF024585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HMZ66085.1"
                     /translation="MNECEYSAGEYIPIVNFSKCEAKGPCIEVCPYDVFELRKIEEKD
                     YNSLTLAGRLKTLVHGKDKAYATRANRCHGCGYCVKACPEKAIQLIKA"
     gene            complement(7647..8354)
                     /locus_tag="PLF66_18495"
     CDS             complement(7647..8354)
                     /locus_tag="PLF66_18495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004476086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HMZ66086.1"
                     /translation="MKTLIVIPAYNEEATIAQVVHGAVKYADVCVVDDASKDKTPEII
                     KGLQRAYPNRVFTVRHEKNTHIPGGIQDGMKFALERDYDYVITMDAGMSHDPAELPKF
                     INYPPNDLVIGRRAKVEGVPFYRRIISFGAARLINYCLSKSIFNLLGPNLRDCTSGYR
                     RYSKTAYSRIAKATLESVAFDFHMEALYLTYSNGGTIREIPITYIFSNSSFNKKVLEL
                     AWAYAKKLLKRKFGMKD"
     gene            complement(8357..9394)
                     /locus_tag="PLF66_18500"
     CDS             complement(8357..9394)
                     /locus_tag="PLF66_18500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011670813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HMZ66087.1"
                     /translation="MIMRVLVTGGCGFLGSHVCETFLKKGWEVVSYDSMTKYELKRTG
                     YGTDATRDFNWNYLKEKGVVMVKGDIRNLEHLLDRTEGCDFIVHTAAQPAMTISWEDP
                     ELDMSTNVVGTFNVLEVARKRKIPVVNTSSIHVYGNSINDSLTESETAYNRNPIEIPE
                     TQPVMVGKISPLHASKMSAEHYVQTYRDMYNLSAATFRFTGIYGERQFGGEDHGWVAN
                     FAIRSVFGLPLRIFGTGKQSRDILYAADGAESYFNFFQNQISGTYNIGGAKEHKISLL
                     ECIYMIGEILGRKQEIIFETERPGDMRYFVCDISMARKFGFDPKVKPREGVTRLIRWV
                     EENKSVFDIKK"
     gene            complement(9391..10884)
                     /locus_tag="PLF66_18505"
     CDS             complement(9391..10884)
                     /locus_tag="PLF66_18505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010576695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HMZ66088.1"
                     /translation="MGETKNSKKVRIGVIAAAGKGTRAYPRTNFIPKPLFKIENKSIL
                     HRNIELLHYKLKIEKIYILVGHLKDQMLEEIEALKYIEPKIVIEAVEWTTKGLASDVA
                     SLESKINEPFILILGDEYYYKTNHEKFLTTYKQNKDITGSIGILRTSVLSRIRKNYSV
                     ELDGKRVKNLVEKPLNPPNEWLGLGSYLFTPKYFEYFKKTPPSQKSGVIEITDVIDLM
                     AKETKQVFATEIECDYFNINSMQDYHYAVYEVRNDLFNQYKISLIIPTRNNERSISDV
                     IVDFKDKVHEILIVDAESSDRTLEIAKSFKTQVEHYFFGDKSEYEGVQIRQGFNRITG
                     DIAIIVSPDGAFRSKDLPKLLEYMKDCDMVVGTRTTRQMIEQGSNLKPLARFIHLAMG
                     KLIEILWWNQEPRFTDVDCRYMAIWKDSYNRIKPSLEVQGKTYTVEMMIEIIRAHMRC
                     IEIPISYFKPVETDSYTWKDAINEALAIMKIAFKKKKEDRKNKKEEE"
     gene            complement(10919..11410)
                     /locus_tag="PLF66_18510"
     CDS             complement(10919..11410)
                     /locus_tag="PLF66_18510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011671401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2505 family protein"
                     /protein_id="HMZ66089.1"
                     /translation="MKYTVTHKFNYSLYDLLRAREDRYKYLDKFPDLKNVTLLEEKKE
                     GNLIFQKRKVSLASSLPPVLVPLLDDASLLEESTFNTDTNTHDFKIFPPKNDKVVTIS
                     GHSVYRSTSDGFSERVYNIEVKSGVLFVGSLVEIAIEEIHKHSLEKDRASIQRFLDEK
                     LER"
     gene            complement(11436..11732)
                     /locus_tag="PLF66_18515"
     CDS             complement(11436..11732)
                     /locus_tag="PLF66_18515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66090.1"
                     /translation="MNQYMIDITLPEYLSEEFVSMIPLQRSIVNRLMGRGVINSYSLS
                     MDRGKIWTVVNAETEKDAERTVGSFPLVRFMRYEIHELAFNNNIAQILPRFSLN"
     gene            complement(11814..13214)
                     /locus_tag="PLF66_18520"
     CDS             complement(11814..13214)
                     /locus_tag="PLF66_18520"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HMZ66091.1"
                     /translation="MQRIFAPIILVLIVLNLSCIAIWEEMNRLSLNEIKKRGKLKVIT
                     SYNANSYFIYKDQPIGYEYDLLKLMTKDLGVELDIQATNDIANLDYMLNSGEGDLIAS
                     NIIVTREKSRQLKFTDHLMTTTQVLVQRKKGMIVRKKMTEHIENAVDLIGKTIHVRAN
                     SDYHNRLKKLQEELGGTIHVKAISENITTDELIEQVYSGEIDYTVADEQTALINNAYY
                     NDLDISLPISFPQKIAWVVRRNSPELLQFINEWIDKNQSTIKEIQDKYYRKSRSYMQE
                     LKIESKPKNKSHLSAFDEIIKENAVVIGWDWRLLAALIYQESKFNPNAKSWVGASGLM
                     QLMPETARNLGVSYAEINNPRKNITAGVKHLKYLDGLWEKTIKDEEERVKFVLASYNA
                     GQGHVIDAMTLARFQNKSATKWDKNVAEAILLLSDPAYYNGYGVRYGYCRGIEPYKYV
                     KVIMEKYAEYRETVKK"
     gene            13501..14010
                     /locus_tag="PLF66_18525"
     CDS             13501..14010
                     /locus_tag="PLF66_18525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66092.1"
                     /translation="MKIFLFLILFSLPLFPVNFNSCDKILSRSLDQIGFKDSVASAKE
                     FAKLYNEYKNKTNIEKCVLDFVKTDCNKNYWVGIFLYEPFFTDIQNYPLAEKIFQSGI
                     KRCSTTCDEIRFKAMFGLLLMRQKKLNYAKKNFVEAADANKKTQGECWPAMTEEESNF
                     IEKEFGVKP"
     gene            complement(14171..15091)
                     /locus_tag="PLF66_18530"
     CDS             complement(14171..15091)
                     /locus_tag="PLF66_18530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002188075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HMZ66093.1"
                     /translation="MKPSLLKSIQSKKRELDKYRPLDKAIVNKLEEQFLLEWTFNSNA
                     IEGNTLTLQETDLVLKNGITIGNKTLREHFEVVNHKAGVEFLYNSIKKKTKLSKEFIL
                     KLHELILTKIDDENAGVFRRTQVRILGSKHIPPSPYKIHSLIEEMVSWYYQNYSKLTI
                     PELAAWIHFKFVHIHPFIDGNGRTARLLMNLVLISHGYPPAVILFLDRKKYYRVLREA
                     DEGKYDSYMDFIGRSIERSLIIYLQAIRPAKSNNKQGYITLSEATKYCDYSLEYLSLL
                     ARKGRLPAVKLNRNWMTTREAIENYIKELK"
     gene            complement(15191..16147)
                     /locus_tag="PLF66_18535"
     CDS             complement(15191..16147)
                     /locus_tag="PLF66_18535"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HMZ66094.1"
                     /translation="MQRFRIKISFLFILFILLGCKDHANNIADNKELLLPSESQISKV
                     LKSKEDYTLLNDLAKSKGYLNAKEYQQKIANDVSNEEFQFYYKKYKPNGLENLGVEAL
                     ANERRKILNRLAWTKMIEEVNIRVELREIEFKKIDWKQISTEDDPTIGNQFAPITVFE
                     FSDFECTFCSKSQKTSKEIRAKYGKKIFWVFKDFPLQEIHTDAFRAHVSANCVKHMEP
                     TKYWEHFNTLFENYRSLEKENLIEYVRENGIDEKKWDLCMKDSGMQKKIISEIQSDLK
                     EGKSLGIKGTPTFIVNGNIIVGARDYDFLTAIIEKELKNTVQ"
     gene            complement(16128..17507)
                     /locus_tag="PLF66_18540"
     CDS             complement(16128..17507)
                     /locus_tag="PLF66_18540"
                     /inference="COORDINATES: protein motif:HMM:NF012544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HMZ66095.1"
                     /translation="MVEEKKELKRGLESRHLQLIALGGIIGSGYFLGTGYVIEKAGPG
                     SFIAYLLGGLIVLCVMYCLGELAVSSPVSSSFIQYASEYISPSWACGVGWSYWVTWVS
                     YVPSEMIAAGIIMSNFFPIFSPVIWAILFGIFVTLVNIVNVNAFGELEFWFSIIKVLA
                     ILLFICLAVLAFLGYSGKQVFVGTSVLLGKGGLFPKGVWAIFLTMVIILVNFQGSEII
                     GLAAGESKDPQKTIPIAIRNVTWRIIALYVIPVFLLVCIFPWNEISLDGSVFAEALSH
                     YGFHWASGIFSFVVLTAAISCSSSGLYGCTRALYALSAQGMAPSYFARLSNNNVPKNA
                     TFFSLVGCWLGILFYTIMGEGIYKNLLALSGFSGAIAWISICWSQLKFRRSLEKTPNG
                     VKDLKFKIPFFPYITHFAIWVQVACLVVMWFNEDLRIAFYIGLPLLVIPIVWYYLRRK
                     RIKDATIQN"
     gene            17604..19319
                     /locus_tag="PLF66_18545"
     CDS             17604..19319
                     /locus_tag="PLF66_18545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020776452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HMZ66096.1"
                     /translation="MNDTVFFSKHNPGLAPFDISNYHGVAGRNFFDEDRLLHHLLDKY
                     TKDYNQSHKEDMLKHIRGYGELVGGILNKLTEACHKEGKYGEVIHFDRVGNRIDKVVY
                     SEEQLQSRKISYEYGIVNLDFHRNWKHPFTTLHKLTLAYLANQNGEGGVTCPLAMTDG
                     IILGIKALGTDEQKKKFLPLVAGEHSSSHFMAGQYVTERVGGSNVGANRTVAKKLSNG
                     KWLLNGEKWFCSNPGDLWVTTARIEGTNTIGMFLVPRLKDDGSLNGCYIIRKKDIIGS
                     RGKITVETVYEDLEAEELGRVSHGLANLIKYIIKTSRIHLSLAALGIGRRALMEGAEY
                     VQVREAYGKKVIEFPSIQKQVAEMKILQSACAVTVFKNVSLLDSENPLLQILNPLMKY
                     ISSSHATWISKQAILLHGGNGILGDFSCLPRLHNDSIINETWEGTHQVISEHVIKAFK
                     RPKAHNAFFEEIQKNIEDSEKYPFLTYANETLKILKARLDTILNSNDELYIEMNRISL
                     CDSIYNLFALSELICEATSYHKESALSHIANGFAEIAMRGKEGLTDQNGIFHKPEILK
                     WIIEY"
     gene            19319..20212
                     /locus_tag="PLF66_18550"
     CDS             19319..20212
                     /locus_tag="PLF66_18550"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HMZ66097.1"
                     /translation="MKQSRSRLLLGFLWLSLSISLGVWWTVMGLTQANQVAELKSYLG
                     AREESVATLERQSRMIKMEGGFFMFLLIVGGSTLIFLSQKDLERTQMLKDFFATLTHE
                     MKTPLASLRLQAESLEEDLKSKKTKAILSRLIDDSKRLELQMDKALYLAALTRKEILY
                     LEVVNLSELISQITSYYPFVSINTIQTSSVKLDVRAFESVIKNIIENAKNHGKATKVT
                     FKIEKISNQAKITISDNGIGFQGNTNELGKLFFRHSTHSGSGIGLYLVKNLIRKMNGH
                     VHFRNNEKGFEVTLHLPVVKN"
     gene            20209..>20765
                     /locus_tag="PLF66_18555"
     CDS             20209..>20765
                     /locus_tag="PLF66_18555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002755804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HMZ66098.1"
                     /translation="MKALKLLLVEDDTSLATTLKERLSKEGYSICWVNTLTKAREELK
                     SSHPNLIILDVGLPDGSGFDLAKELQEKNSQIPFLFLTALASAPERLKGFELGAEEFI
                     PKPFHLKELLLRVKHVLDAHKHVRDSLQKKILFHGYVIDFESYQIFTPDEKKISLSAR
                     DSGLLQLLINEKHRVVSRDEILDKL"
BASE COUNT         6880 a         3865 c         3322 g         6698 t
ORIGIN      
        1 ccttcggatg tgcaatactt cgatgcatct tccgctagtc ggcagaagtc ttagataatt
       61 tttaaatttt ccctacactc aacccaattt gtccgcttgc tctgtagaat aaaaccatat
      121 cactgaaagc atccgtttgt ataaatcaat agtcattcta aaccaaggag agtaaaatga
      181 gcatctacca agacaaaatc aacttagttt acagaaaaga aatggtttct cagcaaaagg
      241 tcggagcttc tagaagtcct aataaaccta aatatctaat agattttcta acacaaaatg
      301 gaatgataac gcattttact ctctatgaca aatgggatcc atttcaaaaa gaagattttt
      361 atttagctca taccaagcaa tacgtagatg ctttcttcgc aggcaaagaa ccgttagcct
      421 cttccaacgg attaaactgg aatgctcaat ttgctgattc agttcgatat acgaatgcat
      481 ctctatttca tgcaatcgaa caaagtattc ttcatccgga tacaatccaa ttttccccta
      541 cttccggatt tcatcacgca agtccttctc acggatctgc cttttgcact ttctccggtc
      601 aagttatcgc ttcagtcaat atatggaatc aatataaact aagaggggct tatatcgact
      661 tagatggaca ttttggaaat tctattgaag actcaaaagg atttataaag gggctaaata
      721 agattatccc ctacaatatt aatccgacta acgcacataa agtttatatt gaaaatttaa
      781 ctaaagaact aaatcgtctt tcccttgatc tcctttctgg aaatgtagat tatattgtat
      841 tctgtcatgg agcagactcg catgaatggg acgaccttgg gcatcaatgc agcacagaag
      901 aatggctact ctgttcagag ttagtctata aaatgattca tcgcctttct aataaattgg
      961 gcaaaccaat tccattgtcc cttagtctct ttggcggata tcgtagtgac gattttaatt
     1021 cggttctgag tcttcacact gccgatcttt ctagatgctt agatattctc tgcggtcaga
     1081 atactcagta tttgccgata gtgaaagaac ggtaaaagat cattgtcaat tgggacgatt
     1141 gaatgagaga acacattggt cacgaataca taaaacgaac acaggattaa acgatgcgcg
     1201 aatgaaaaat atcaatcgtt ttttatcgtt ctgctatcgg tgtgaatcgg tggtaatctt
     1261 taagataaat cagccatccc aatatgacat ttatcagcaa tttcttccaa ttgcctgaca
     1321 gttccatgac aattccaaaa tcctatatgg tattatattg gcatgtggtc aaatttgctt
     1381 attctacatt caaatgataa aaatcgagat agagaaagct ttcatggatt gcaccattgg
     1441 aagacttgta tgcgaaccct ttacattggt gatattcgca tttttgattt atcacaggct
     1501 caattctccc gccactatga agcttacgta ggatatgaag catataaatt gcttttagaa
     1561 attgtgagtg gaatccgttc ccgtttactt ggggaaacag aaattctcgc tcaattcaaa
     1621 gagaccttaa aaaatgaagc ccttcccgaa cattcactcg gagactattt aaaaaaacta
     1681 agagatcaaa tcattgagga ctctagaaaa atcagaagtg gtcatcttcg atttttagga
     1741 gatcaatcct atggtgggct tgcaaatcgt tatttaaaaa acacaaaaga agtaactctt
     1801 tttggaaccg gaaatttagc agtgaaagtt ttaccatggc tattagaaaa aaatagaaaa
     1861 gtaaaagtag ttggaagaaa cttagaaaaa ctaaaagaca tcgcaagtaa atttccagtt
     1921 cagattcaga cgttagacga atacaaagta gcggatgaag cgattgtaat agctgctcct
     1981 atttctatga aagagtggat tccttctttt tctgaagata aaatcatcat tgatttcaga
     2041 gaggatgaca aaaaagacga cttctctaaa aacaaaaatt atatttcttt tgaattcatg
     2101 ctgaattctc tcaaggcaca agaagaaaga aatgcagagc ttagaaaaaa attagacgta
     2161 gttatacaag aattagccgc tgagcgagaa aatgaaagta ttaactttat ctatggctgg
     2221 gaagatatac cttgtctctc tcactaaaga ttggttctag aaaaagcgct ttagccaaat
     2281 tacaatctta cttagttgca aaggctttaa gagaaaaatt tccaggcatt caaattgaat
     2341 tctatttcaa ggaatcgtta ggagacaaag acctaacttc tcctctttgg aaaatgggag
     2401 atcgtggcgt atttacaaaa gacttcaaag aagatttact ttccgaaact gtagatgtag
     2461 tcattcattc ttggaaagat ttagatttac aacatgaaga caatacagaa gtaatttcaa
     2521 tcttagaaag agcagaccaa agagatttac ttctttttaa aagagaacat ttattaaatc
     2581 caaactttga tgaaattaaa atattcagtt cctcacctag aagagaatac aatctaaaac
     2641 gatttttaag caaagctctt cccaaaagac tgcatggcaa gccaattcta tttgagccag
     2701 tccgaggcaa tatgcaaaca cgtcttgcaa aatggaaaca ggctgatact gtccaaggct
     2761 tgatacttgc aaaagctgca cttgatagac ttctcgcaac taactttcct gagtccgaga
     2821 gtgacgaata ctctgaaatt agaaaaacaa ttcgagccta tttaaaagaa tctgtattta
     2881 tgtgtttgcc tttatctgaa aatccaaatg cacccgcaca aggagcgtta gccgctgaga
     2941 ttaaagcatc cagatctgat attagagaga ttatttccaa actcaccatt cactctgtga
     3001 gtagatccgt aaacatagag agacaagaat tacaaaaata tggcggtggt tgtcatcaaa
     3061 aaattggagt cagtgcaatc gctaaaaatt atggagaagt attttatttg agaggactca
     3121 ctgatagtaa tgtcgaactc aactcccata aaattttaaa agaaaatcca tctcaaccta
     3181 aagccaaacg aatgactgat atttttccga atgaaaagga aaagtttaag ttcaaaagag
     3241 agtctcttcc tgaacctgaa atcccgaatt caaatatact cgtttctaga accaatgcct
     3301 ggcatccaaa ttggagtcga gttgacttgg atcaaatcat ttgggcagct ggtattaaaa
     3361 ccatgtatca actagcagaa aaggatttat gggtaagtgg aacgttagac ggattaggag
     3421 aagaagaatc tcccaatatt tcaatgcttg tagagaataa taaacccttt gttaaactca
     3481 cacatgaaga tagcagtgag atagaatctg agatggaaag aatttataca tataaaatct
     3541 ttttagaaga agaaattcct gatctctcaa acaaaactca tttcttttgg atgagtggtt
     3601 atcaatttga tctagcacta caaaagtatc ctgaaataat acataaattc catgcttgtg
     3661 gaccaggaat tacagccaaa catatacaga aaaaattagg ctcgaattct tctcttgaga
     3721 tttttctaaa ctatgaagat tggcttcatt ttcatacaac tgactaacgt tctggttaag
     3781 ttttttaaat catctgaatt aaagtctttt caaataaaga aataagtatc cctaatcaga
     3841 atttcttcgc caaaaagccg ccaaaacact cgcaatcaaa gaatgcgcta aacttgaaat
     3901 agcgcaaggc actgctgtta gaggattcga gaaattctcg cgcgctaata caacaccgag
     3961 accagaattt tgcatcccta cttcaatcga tatggttctt gcagaaattt ttctttcctt
     4021 catgagaaca ctcaagacat acccaaagaa aaatccaaat atgtgaagag taaatacaga
     4081 agctaaaagt cggattcctg actcaataat tggcgctttg ttagatgcga ttacagaggc
     4141 tacaattaaa ataatcgaaa caacggcaag aagtggagag atgttagaaa ttttttttgt
     4201 agctttggga aatagaaaat ttaagaaaac tccaattgca acaggaagta taatcacttt
     4261 taaagtgcta aataatagtc cagatgcatt tacatccact cgatttcctg ctaaataaaa
     4321 cgttaggatt ggagtcatga ttactgaaat gagtgttgaa atagcagtca tagtcacaga
     4381 gagagccaca tctgcttttg ctataaaagt taatacatta gatgcagtcc ctcccggaca
     4441 gcaggcaact agaattagcc ccacggcaag tggatttggt aaatcataaa gtaaagctac
     4501 aatatagcca gataccggca ttacagtata ttgcagaaat ataccaataa aaacggattt
     4561 tggaaatttt aaaatccgtt tgaaatcatc tagtgtaaga gtaagcccca ttccaagcat
     4621 gataattcca agtgcaaagg agatatactc tcctttaaac caagaaatca aatttggata
     4681 aataaaagcc gctatagata caatcagaat ccatagcgga aaaagtgacg ttaattgata
     4741 gatcaattta ttctaagctc atattgagag aattaaaatt taaattgttt gagcgcttta
     4801 aatttcgaac cacaaaagta tcatttacct tttcaagttc taaaaactct gcaaatattt
     4861 tttcgatttt attcggatct cctccagaac ctaaggtatt acaatctcta ccacatccat
     4921 tgcaatccat gtgctctatt tgcatttccg atttaatttc tcttaaaaaa ccaaagattc
     4981 taacttgtaa gatattattt tcagttgact ttacttcata gttgcctaac gcataaaaac
     5041 gactcgtatt ttctgaatca gaatcataag tagtagtgct tccttctaaa aacatacttc
     5101 catcggaacg aagtctaact gtccaattag aattcacacc ctcttctgag cctgttaaac
     5161 ttttatttaa gatttcagag attccagctt tttgaaaatc atctccattt gtttttgcta
     5221 tcgatttgat tctaggaaac ggatctaaaa gaaaccattc tttcccatca aagaatctaa
     5281 gctcacttag aacgatgcct tggtattttt taccacgata ggattcatcg attgtcataa
     5341 ctaatttttt accgttaaaa ggttttggga gttctacgat ttgcggtccc atttcatcct
     5401 tcaaagaaat tttttcactg tatccttcat ctccggtgag gttaatcgtt tttacccgcc
     5461 cgttttcaac acaatgcaca tcagatcttt gatacccatt ccatactttt atagattgaa
     5521 tcttttgtgt atctttgaaa ttaaaagaga gactaacgcc tttcgaatta tttaaagatg
     5581 cataagaata ttcatatctg gaatcaaata aattcataac tccataagaa gcttctggct
     5641 ttccagtctc agatgcttct gcagttcctt ctacaatacg aggagctttc accttaaact
     5701 gttttccttt ttcatcaaat aaatgaacag aagctaaaca gatatttgaa ttcttagaaa
     5761 aattgaatgt aacagatcta gctgttacag gactctcgaa actatgagca gctaccttac
     5821 cctgcgcttt cattgtttga atcatttcat caaagttgat atacatatca atctgagatt
     5881 taaaatcgtc agaacatgaa ttgacttcta ccttagataa ttgaactgct tcatctaaat
     5941 aaacatgtaa cttcacaaac tcggcattag cctctggcat ccattttgaa tcattcaatg
     6001 caagaaacgg cagaccattt tgcatagatg ttgcagtgac catcgaaata gaaacttcct
     6061 ttttacagcc aataaaaaga aaaagacata gggtaaatat atattttttc attttggttt
     6121 atctcctctt ctcaaatttt gatttaaaaa ccttttcgtc aattggaata tttacttctt
     6181 gtttgcataa attctgtaac tcacttataa ttgaaagaat caaaaggcta aactctcaaa
     6241 tacaaatcaa ttcactttat caagtgtcat tactgatgtt taaaattctt tatttttatc
     6301 agtgggagtc tttcaaactc tttgctacac cgaactgacg tttttaatct atataaatca
     6361 ataatttaaa atagttcctt catctcacgg ttaatctcaa ttgccttctc gcgagccttc
     6421 aaagcaaaat cagattcatt tgaattgaat agaatagagc gagaggaatt gattaaagaa
     6481 tttcgagcag atactttcat tatatcactg agatttccac cttgcgctcc atagcctgga
     6541 attaaaaatg caagttcagg aaaagcgttt cgaatttgcc ccaattcatc tggatgcgta
     6601 gctcctacaa caagccctac ttgatcttta aatttagaat tcagcgactt actaaagtta
     6661 gccacttcct cataaaaata ttttccattc acagttttaa ttttttgcaa atccttagaa
     6721 gcaggatttg aggtaaggca aagtaaaaac accgcatgac ctttgtattc aaaaaatggt
     6781 tcaatactat cagaccccat atacggagac aatgtgagtg aatctacttt caaatctcca
     6841 aaatagtatt tcgcatactc ctttgctgtg ttcgcaagat cacctctctt aatatctgcc
     6901 acaattgcaa cctgtggata attagatgtt atatgacgaa ttaatttttc aaattgctct
     6961 attcctttaa atccaaatcg ttcaaagaaa gcaatattgg gtttgtatgc gatagtaata
     7021 tcgtgtgttg catctacaat ttctttacag aaagtataaa gagcattctc gctatttttt
     7081 atggaagctg gaagtttttc ccattctgga tctagaccaa tacagagtaa acttcttaaa
     7141 ctattagatc gtgttattat tttctgataa aaactcattt ttaaaaacct gcttattaat
     7201 tgcatgtaaa ctcgatatac ggatttagca agtgatttaa aatgtcactc cgaatttcca
     7261 tttatagttt ttagcaatcg gcatcaggaa tctatttctc agatttaaaa aaatggaact
     7321 ttttcaaaga aggttggtct gatcgtaaat gactcaaaaa gagattgaaa tatgaatgaa
     7381 tgtgaatatt ctgcggggga atatatccca attgtaaatt tttccaaatg cgaagccaaa
     7441 ggtccttgta tagaggtttg tccatacgat gtttttgagc tcaggaaaat tgaagaaaaa
     7501 gactataata gcttaacgtt agcaggaaga ctaaaaacct tagtccatgg caaagacaaa
     7561 gcctatgcta ctcgggcaaa tcgatgtcat ggatgtggtt actgtgtaaa agcctgtcca
     7621 gaaaaagcga ttcagcttat taaagcttaa tctttcattc caaattttct cttgagtagc
     7681 ttttttgcgt atgcccaagc aagctctaaa acttttttat taaaagagga attagagaat
     7741 atataagtaa ttggtatttc tcgtatcgtt cctccatttg aatacgttaa ataaagagct
     7801 tccatatgaa aatcaaatgc aacagattca agtgttgctt ttgcaattcg agagtaagca
     7861 gtttttgaat aacgtctgta gccagaagtg caatctctaa gattaggacc aagtagatta
     7921 aaaatacttt tagaaagaca ataattgata agtctagctg caccaaaaga aatgatccta
     7981 cgataaaatg gaaccccttc cacctttgct cgtcttccta ttaccaaatc atttggtgga
     8041 taattaataa actttggtag ctcagcaggg tcatgagaca taccagcatc cattgtaatt
     8101 acataatcat aatcacgctc aagcgcaaac ttcataccat cttgaattcc acccggaata
     8161 tgtgtgtttt tttcatggcg aactgtaaag acgcggttcg gatacgctct ctgtaaacct
     8221 tttataattt caggagtttt atcttttgat gcgtcatcta caacacaaac atctgcatac
     8281 ttgactgccc catgcacaac ctgtgcaatc gtcgcttctt cattatacgc tggaattaca
     8341 ataagtgttt tcaagattat ttcttaatat caaaaacaga tttattttct tctacccagc
     8401 ggataaggcg tgttacaccc tcgcgaggtt taacctttgg atcaaaacca aatttacgag
     8461 ccatggaaat atcacataca aaatatcgca tatcgcctgg acgctctgtc tcaaaaatga
     8521 tttcttgttt acgacctaaa atttccccga tcatataaat acattcgagt aaggaaatct
     8581 tatgctcttt tgcaccgcca atgttataag ttccagaaat ttgattttga aaaaagttaa
     8641 aataactttc tgctccatca gctgcatata aaatatctcg cgattgttta cctgttccaa
     8701 aaattctaag cggtagacca aaaacagagc gaattgcaaa gttagcaacc caaccatggt
     8761 cttctccacc aaattgtctc tctccataaa ttccagtaaa tcgaaaagtt gccgctgaga
     8821 ggttatacat atctctataa gtttgcacat agtgttctgc actcatttta gatgcgtgca
     8881 aaggagaaat ctttccaacc attacaggtt gtgtttcagg aatttcgatt ggatttcggt
     8941 tgtatgcagt ttctgattca gtgagagagt cattgatcga atttccatac acatgaattg
     9001 aacttgtatt aaccacagga atttttcgtt ttctagctac ttctaaaacg ttaaaggttc
     9061 cgacaacgtt agtagacata tctaattctg gatcttccca agagattgtc attgctggct
     9121 gtgctgctgt atgaacgata aaatcacaac cttctgttct atctaggaga tgttctaaat
     9181 ttcgaatatc tccttttacc ataactacac ccttttcttt tagataattc cagttaaaat
     9241 ctcttgttgc atccgtgcca taaccagttc tttttaattc atatttggtc atgctatcat
     9301 aacttacaac ttcccagcct ttttttaaaa aagtctcgca tacatgagat cctaaaaatc
     9361 cacagccgcc tgttactaat actctcataa ttattcctct tcttttttat tttttctatc
     9421 ttcttttttc tttttaaatg ctatcttcat aatggctaac gcttcgttta tcgcatcttt
     9481 ccaagtgtat gaatccgttt ctaccggttt aaaataagaa attggaattt cgatacaacg
     9541 catatgtgct ctaataattt caatcatcat ctctactgta taagtcttac cttgcacttc
     9601 aagagatggt ttgattctat tataagaatc tttccaaatt gccatataac gacaatctac
     9661 atctgtaaat ctaggctctt ggttccacca tagaatttcg atcagctttc ccattgctaa
     9721 atggataaaa cgagcgagtg gcttaagatt agaaccttgc tctatcatct gacgtgttgt
     9781 tcgggttccg accaccatat cgcaatcctt catatactct aagagtttag gtaaatcctt
     9841 agatctaaat gctccgtccg gtgaaacgat aatcgcaata tctcccgtaa tccgattaaa
     9901 cccctgtcta atctgcactc cttcatattc agatttatca ccaaagaagt agtgttcaac
     9961 ttgagtttta aaggactttg caatctcaag agttctatcc gaggattcag catctacaat
    10021 tagaatttca tgcactttat ctttaaagtc tacgattaca tcggaaattg atctttcatt
    10081 atttctagtt gggatgatta gagatatttt atattgattg aataaatcat ttctgacttc
    10141 gtagactgca tagtgataat cttgcattga attaatatta aaataatcac attcaatttc
    10201 agttgcaaaa acctgtttgg tttcctttgc cattagatca atcacatccg taatctctat
    10261 cactccactc ttttgagatg gtggagtttt tttgaaatat tcaaaatact ttggagtaaa
    10321 taaataagaa ccaagtccaa gccattcatt tggtggattt agaggtttct caactaaatt
    10381 tttaactctt tttccatcca attctacaga ataattcttt cgaattctag aaagcacaga
    10441 tgttcgtaaa attccaatcg aaccagttat atccttattc tgcttgtaag tagttaaaaa
    10501 tttttcatga tttgtcttat aataatattc atcgcctaaa attaaaataa aaggttcatt
    10561 tatttttgac tcaagggaag ctacgtcaga cgcaagaccc tttgttgtcc actcaactgc
    10621 ttcgattaca attttaggtt cgatgtattt tagagcttca atttcttcaa gcatctggtc
    10681 ttttagatgc ccaactaata tataaatctt ttcaattttt aatttataat gtagaagctc
    10741 aatatttctg tgcaggattg atttgttttc gattttaaaa agaggtttgg gaataaaatt
    10801 tgtgcgtgga tatgctcgcg tgccttttcc agccgctgct attacaccaa ttcttacctt
    10861 ctttgaattt ttagtctcgc ccattctcgg acttcttttt aaatatctat attattgttt
    10921 atctttctaa tttttcatct agaaatcttt gaatggaagc tctatctttt tctaaactat
    10981 gtttgtgaat ttcttcgatc gcaatttcga caagacttcc cacaaataaa actcccgatt
    11041 ttacttcaat gttatagact ctctcagaga atccgtcgga agtagaacga tatacgctat
    11101 gaccagaaat tgtaactact ttatcatttt taggtggaaa tattttaaaa tcatgtgtgt
    11161 tagtgtctgt attaaaggtt gattcctcta ataaagatgc atcatctaac aatggaacta
    11221 atacaggtgg caaggaagat gcaaggctaa cctttctttt ttgaaatatt aaattacctt
    11281 ctttcttttc ttctagtagt gttacatttt ttaaatctgg aaatttatct aaatatttat
    11341 atctatcttc tcttgctctg agcaagtcat atagagaata attaaactta tgcgttactg
    11401 tatatttcat taaaaataaa acccttttat aaaaattaat ttagtgaaaa tcgaggtagg
    11461 atctgtgcaa tattattatt aaatgcaagt tcatgtatct cataacgcat gaaccttaca
    11521 agcggaaaag atccaacagt tctttcagca tctttctcag tttccgcatt tacaacagtc
    11581 caaattttac cacgatccat ggaaagtgaa taactgttaa taactcctct acccatcaaa
    11641 cgatttacaa tggatctctg aagtggaatc atagatacaa attcttccga aagatattca
    11701 ggtaaagtaa tatcaatcat atattgattc atgagtaatt tctccttgga cgactgttaa
    11761 tcccattggt atcgcataca aacttactgt caagggtaaa tcaaaacatt ttcttatttc
    11821 ttgactgtct ctctatattc agcatatttt tccataataa ccttaacata tttataaggt
    11881 tctattcccc tacaataacc atatctaact ccatagccat tgtagtatgc tggatcggat
    11941 aacaatagaa ttgcttctgc cacattctta tcccatttcg tagcactttt attttgaaaa
    12001 cgagccagag tcatcgcatc aatcacatgc ccctgacctg cgttgtagga ggctaaaaca
    12061 aacttaactc tctcttcctc gtccttgatt gttttttccc aaagtccatc aagatattta
    12121 agatgcttta ccccagctgt aatatttttc ctaggattat tgatttctgc ataacttaca
    12181 ccaagatttc gagctgtctc tggcatcagt tgcataagcc cgcttgcacc cacccaagat
    12241 tttgcatttg gattaaattt tgattcttgg taaatcaacg cagccagtag cctccagtcc
    12301 caaccaatca caacagcatt ttcttttatg atttcatcaa aagcagaaag atgagatttg
    12361 tttttaggct ttgattctat cttgagttcc tgcatatagg atctagactt gcgatagtat
    12421 ttgtcctgaa tttctttaat tgtagattga tttttatcta tccattcatt gataaattgt
    12481 aaaagttctg gagaatttcg tctaacgacc caagctattt tttgagggaa actaattggt
    12541 agactaatat ctaaatcatt gtaataggca ttgttaatca aggcagtctg ttcatctgcc
    12601 acagtgtaat cgatttcgcc gctatacact tgctcaatga gttcgtctgt tgtaatattt
    12661 tctgaaattg ctttgacatg aattgttccg cccaattctt cttggagctt ttttaatcga
    12721 ttatgataat cagagttggc tcgaacatgt attgtttttc cgattaagtc cactgcattc
    12781 tcaatgtgtt cggtcatctt ctttcgaaca atcatacctt tctttctttg gactaagact
    12841 tgtgttgttg tcattaaatg atctgtgaac tttaattgcc tgcttttttc tcgggtaaca
    12901 attatattgg aagcgattaa atccccttcc ccactgttta acatataatc gagatttgct
    12961 atgtcgttag tcgcctgaat atccaactct acacctaagt ccttggtcat cagctttaat
    13021 aaatcatact cataaccaat cggttgatcc ttgtagataa aataactatt cgcgttgtag
    13081 gatgtaatga ccttaagctt tccccgcttt ttgatttcat ttaagctgag tcgattcatt
    13141 tcttcccaga ttgcgataca agaaagattt aaaacaatca atacaaggat gataggtgca
    13201 aaaattcgtt gcataggttt aattaaacta gaaatttaat ctgaaattgt aaagaaattt
    13261 atttttgaaa ataaaaagat tcccacagat gaaaacgatg aacactgagg taaatagctt
    13321 tttctatttc aactcacagt attttgggaa tctacaaatt aactctttaa aaacttctac
    13381 ttttttgagg aaatcgaatc ataaattttg tttaattctt tatccttgtt tttgtatgtt
    13441 ctcaatttca agagtttgag caatgattta caaatatctt gaatgcttta aactggttct
    13501 atgaaaattt tcctattctt aattttattc tctctccctt tgtttcctgt aaattttaac
    13561 tcttgtgata agatactctc tcgctcacta gatcaaatcg gtttcaaaga cagtgtagcc
    13621 agtgcaaaag aatttgctaa actttataac gaatataaga acaagaccaa tatagaaaaa
    13681 tgtgtcttag attttgtaaa aacagactgt aataaaaatt attgggtagg aatttttcta
    13741 tatgaaccct tttttacgga tattcaaaat tatccactcg cagaaaaaat ttttcagtct
    13801 ggaatcaaac gatgttctac tacctgtgat gaaatccgct tcaaagcaat gtttggactc
    13861 ttgctcatgc gtcaaaaaaa attaaactat gcaaagaaaa attttgtgga agcggcagac
    13921 gcaaataaaa aaactcaagg cgaatgctgg cctgctatga cagaagaaga atctaacttt
    13981 atcgaaaaag aatttggagt gaaaccttag agttagtata caaattctca aaggaaaaaa
    14041 ctcaatttca aaaaaagctt tatttttaga aattcagaat ccatattttt tttataatgg
    14101 gagaatcatc caaccatcaa agcaagtcca taatcatcga tggcagatca attctttcct
    14161 gaaatttgta ttattttaac tctttgatat agttttcaat tgcttctcta gttgtcatcc
    14221 aatttcgatt taatttgact gcaggaagtc ttccttttcg agcaagtaaa gataaatatt
    14281 ccaaggaata atcacagtat ttagttgctt cgcttaacgt tatatatcct tgcttattgt
    14341 ttgacttcgc tggacgaatc gcctgcaagt agataattag agatctctcg attgaccttc
    14401 caataaaatc catataagaa tcatatttgc cctcatccgc ttctcttaaa actcggtagt
    14461 atttttttcg atctaaaaaa agaatcactg cgggaggata tccatgcgaa attaaaacta
    14521 aattcatcaa gaggcgagct gttctaccat tcccatctat aaatggatga atgtgcacaa
    14581 acttaaaatg aatccaagca gccaattctg gaatggttaa tttagagtag ttctgataat
    14641 accaactcac catttcttca atcaaggaat gaatcttata tggacttgga gggatatgct
    14701 ttgagcccaa aatccgaacc tgagtcctcc gaaagactcc tgcattttca tcatctattt
    14761 ttgtaagaat caattcatgc agctttaaaa taaactcttt gctgagtttg gtttttttct
    14821 tgattgagtt atataaaaat tctactcccg ccttgtggtt tacaacttca aaatgttcac
    14881 gtagagtttt attcccaatc gtgattccat tttttaatac aaggtctgtt tcttgtaagg
    14941 taagagtatt accctcaatt gcattggaat taaatgtcca ctcgagcaag aattgctctt
    15001 ccaatttatt tacgatcgct ttatcaagcg gtctatattt atctaattcc ctttttttag
    15061 attgaatgct tttgagtaaa cttggcttca tatcggataa gtctattttc aacttatccg
    15121 atacgaatgt caataactaa acgattcgtg tatcggataa ttcatatttt aagccgatcc
    15181 gatatagcat ctattgaaca gtatttttaa gttccttttc tatgattgcg gtaaggaaat
    15241 cataatccct cgctccaacg ataatatttc catttacaat gaatgtagga gttcctttaa
    15301 tacctaaaga ttttccttcc tttaaatcag attgaatttc tgagataatt tttttttgca
    15361 ttcccgaatc tttcatgcat aaatcccact tcttttcatc aatcccattt tctcttacat
    15421 actctattaa attttctttt tccaaacttc gatagttttc aaacaacgta ttaaaatgtt
    15481 cccaatattt agttggttcc atatgcttca cacaattggc agatacatga gcacgaaatg
    15541 catccgtatg aatctcttgc aatggaaaat ctttaaaaac ccaaaagatt ttctttccat
    15601 atttcgcacg aatttctttt gaagtttttt gtgacttaga gcaaaatgtg cattcgaaat
    15661 cggaaaattc aaagacagta ataggagcaa attgatttcc aattgtagga tcatcctctg
    15721 tcgaaatttg tttccaatcg attttcttaa attcaatttc ccttagctca actcgaatat
    15781 ttacttcttc gatcatcttt gtccaagcca gtcgatttag tattttcctt ctttcatttg
    15841 caagtgcttc tactcctaga ttttctagcc catttggttt gtatttctta tagtaaaatt
    15901 gaaattcttc gtttgaaaca tcattcgcaa ttttctgttg atactctttg gcatttaggt
    15961 atccttttga ctttgctaag tcattgagaa gagtatagtc ttcttttgac tttaaaactt
    16021 ttgaaatttg actttcagaa ggtagcagca attctttgtt atccgctata ttatttgcat
    16081 ggtctttaca acccaaaaga atgaatagaa tgaataaaaa actaatttta attctgaatc
    16141 gttgcatctt taatcctttt tcttcttagg taataccaca ctattggaat tactaagaga
    16201 ggcaaaccaa tataaaaagc aattctgaga tcctcattaa accacatcac aacaagacat
    16261 gctacttgca cccatatagc aaaatgcgtg atataaggaa agaatggaat tttaaatttt
    16321 aaatctttta ctccatttgg agttttctct aaactccttc taaatttcaa ttgagaccaa
    16381 caaattgaaa tccaagcaat agctccacta aatccagaca aagctaataa atttttataa
    16441 attccctccc ccataatagt ataaaaaaga attccaagcc aacatccaac aagcgagaaa
    16501 aaggtagcat ttttaggaac attattattg ctgagtcttg caaaataaga tggtgccatc
    16561 ccttgtgcgg ataacgcata caaagcacga gtgcatccat ataatccaga actagaacaa
    16621 gaaatcgcgg ctgttaagac aacgaaagaa aaaatcccac ttgcccaatg aaatccataa
    16681 tgtgataatg cctctgcaaa aacacttccg tctaaactaa tttcattcca agggaaaata
    16741 caaaccaata aaaaaacggg aattacatat aaagcaatga ttctccaggt tacatttcga
    16801 attgcgattg gaatcgtctt ctgtgggtct ttactctccc ctgctgcgag acctattatc
    16861 tcggatcctt gaaaattcac taaaataatt accatcgtaa gaaatatagc ccaaacgcct
    16921 ttgggaaaaa gtccaccctt tccaagcaaa acacttgttc cgacaaacac ttgtttgccg
    16981 ctatatccta agaatgctag cactgccaaa caaataaaaa gtaatattgc aagaacctta
    17041 atgatagaaa accaaaattc tagttctcca aacgcattta catttacaat atttacaagt
    17101 gtgacgaata ttccgaaaag aattgcccat ataactggac taaatatagg aaagaagtta
    17161 ctcatgatta tccccgcagc aatcatttca gatggaacgt aactcaccca agtcacccaa
    17221 taagaccaac caactccaca tgcccaagat ggggaaatat attcactcgc atattgaata
    17281 aaagagcttg agactggtga agaaacggca agttctccta agcaatacat aacgcataat
    17341 acaatcaatc caccgagtaa ataagcgata aaagaacccg gtcccgcttt ctctattaca
    17401 tatccagttc ctaaaaaata tccacttcca atgattccac caagcgcgat cagttgtaaa
    17461 tggcgagatt ctagacctcg ttttaattct tttttttctt ctaccatgaa attaaacagg
    17521 gaatttccaa tgaaccctga gaagtcagag tagaaaatta gtaaaaagct tgcaattcag
    17581 atttcaaatc aaagtatttc attatgaatg atacagtttt tttttcgaaa cataatccag
    17641 gcttagcccc atttgacatt agcaattatc atggagttgc aggtagaaat tttttcgatg
    17701 aggatagact ccttcatcat ctcttagata aatatacaaa agattataat caatctcata
    17761 aagaggacat gttaaaacat attcgcggat acggagagtt agttggtggc attttgaaca
    17821 aactaacaga ggcatgtcat aaagaaggaa aatatggaga ggtcattcat tttgatcgag
    17881 tgggaaatcg aattgataaa gtagtctact cagaagagca attacaatct agaaaaattt
    17941 cttatgagta tgggattgta aacctagact ttcatagaaa ctggaagcat ccttttacta
    18001 cattgcataa actcacactt gcttatctag ccaatcaaaa tggggaagga ggagtcacct
    18061 gccctcttgc gatgacagat ggaattatac tcggcataaa agcactcggg acagatgaac
    18121 aaaaaaagaa atttttaccg ttagtcgcag gagagcatag ctcctctcat tttatggcag
    18181 ggcaatatgt aacggaaaga gtcggaggat cgaatgtagg agcaaatcgg acagtcgcta
    18241 aaaagctttc gaacggcaaa tggttattaa atggagaaaa atggttttgt tcaaatccag
    18301 gtgatctttg ggttactaca gctaggattg aaggcacaaa tacgattggt atgtttttgg
    18361 ttcctagact caaggatgat ggatctctca atggttgcta tatcattcga aaaaaagata
    18421 tcattggctc tcgcggaaaa attacagtag aaaccgttta tgaagatttg gaagcagaag
    18481 aactaggaag agtctctcat ggtctcgcta atttaattaa atatattatc aagacttcca
    18541 gaattcacct atcgttagcc gccttaggaa ttggaagaag agctctcatg gaaggagctg
    18601 aatatgttca agtcagagaa gcctatggaa agaaagtaat cgagtttcca tctatccaaa
    18661 aacaagtagc tgagatgaaa atattacaat ccgcatgcgc tgtcacagtc tttaaaaatg
    18721 tgagccttct cgattcggaa aatcctctct tacaaatttt aaatccactt atgaaatata
    18781 tctcttcgtc ccatgctact tggatctcca agcaagctat tttacttcat ggaggaaatg
    18841 ggattttagg tgacttttct tgtcttcctc gtttacacaa tgactcaatt ataaatgaga
    18901 cttgggaagg aacacatcag gtcatatcag agcatgtaat caaagcattt aagagaccaa
    18961 aagctcataa cgcatttttc gaagagattc aaaaaaacat tgaagactcc gagaaatatc
    19021 catttctaac ctatgcaaat gaaaccctga aaattctaaa agccagactg gatacaatct
    19081 taaactctaa tgatgaactt tatatcgaga tgaatcgaat ttcactttgc gattctattt
    19141 ataatttatt tgcattgagt gaactcattt gtgaagcaac atcctatcac aaagaatcgg
    19201 ctctatcaca tatcgcaaat ggatttgcag aaattgctat gcgagggaaa gaaggcttaa
    19261 cagatcaaaa tggtattttc cataaaccag aaattttaaa atggatcatt gaatactaat
    19321 gaaacaatcc cgtagcagac ttcttttagg atttctttgg ctctcacttt ctatctcact
    19381 tggagtatgg tggactgtga tgggcctcac ccaagcaaat caagttgctg aattaaaaag
    19441 ctatttagga gcaagagaag aaagtgtagc aacattagaa cgtcaaagtc gaatgatcaa
    19501 aatggaaggt ggattcttca tgtttctttt aatcgtcggt ggttctacct taatctttct
    19561 ttctcaaaaa gatttggaac gaactcaaat gctaaaagat ttttttgcaa cgctcaccca
    19621 tgaaatgaaa actccattgg cttctcttcg attgcaagca gaaagtttag aggaagattt
    19681 aaaaagtaaa aaaacaaaag caatactctc tagactcatt gatgactcaa aaagactcga
    19741 gttacaaatg gataaagctc tttatttggc agcgcttaca cgaaaagaga ttttatattt
    19801 agaagttgtc aatctttctg aattaataag ccaaatcact tcctattatc catttgtttc
    19861 aatcaacaca atccaaacat cttcagtcaa actagatgtg agagcttttg aaagtgtaat
    19921 taaaaatatt atcgaaaatg ccaaaaacca tggcaaggca actaaagtca catttaagat
    19981 tgaaaagatt tctaatcaag caaagattac catatcggat aacggtatag gctttcaagg
    20041 aaatacgaat gagcttggga aattgttctt taggcattca acccacagtg gcagtggaat
    20101 tggtttatat ctagttaaga atctaatccg caaaatgaat ggtcatgtgc attttagaaa
    20161 caatgaaaaa ggatttgaag taacccttca tctaccggtg gtaaaaaatt gaaagccttg
    20221 aaattactct tagtagaaga cgatacatct cttgccacta ctctaaaaga aagattaagc
    20281 aaagaaggtt acagcatttg ttgggtcaat actcttacaa aagcaagaga agaattaaaa
    20341 agttctcacc ccaatttgat tattttagat gttggtcttc ccgatggatc tggatttgac
    20401 ttagcaaaag aattacaaga aaagaattca caaatccctt ttttattttt aacggctctc
    20461 gcgagcgcac cagaaagatt aaaaggattt gaacttggcg cagaagaatt tattccaaaa
    20521 ccatttcact tgaaagaatt attacttaga gtcaaacacg tgttagacgc tcataaacat
    20581 gtaagagatt cccttcaaaa gaaaatatta tttcatggtt atgtcattga ttttgaaagt
    20641 taccaaatct ttactccaga tgaaaagaaa atttctcttt ctgctcgaga ttcaggtcta
    20701 ctgcaacttc taatcaatga aaagcataga gttgtcagtc gagatgaaat tttagataaa
    20761 ctatg
//
LOCUS       DAOQOS010000098        20715 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_1251_length_20715_cov_5.956438, whole genome shotgun sequence.
ACCESSION   DAOQOS010000098 DAOQOS010000000
VERSION     DAOQOS010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 20715)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20715)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20715
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1251_length_20715_cov_5.956438"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1227
                     /locus_tag="PLF66_18560"
     CDS             <1..1227
                     /locus_tag="PLF66_18560"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HMZ66099.1"
                     /translation="IKGRIQIAEIKYTGPPAQSWIVSGIRDTVTNDLAKIKEVSAISI
                     EDQREAFKLIALKQKTGVNVDASIEAAKILAADYLCVGNLQNTGKTLRLNIRLLKSPD
                     FSAEQSTTIDGTLDEIFTLQDKVVESLLANVDAKMTPEERLVIEIFTPKNKNAYELYS
                     QGLEIQDTNPKKALDLFLQALKLEPDYLDALNRVGNQYDSFNNFSEALNYHEKRRKIL
                     EEKGLSKHERYSETLNNMGAVYYSQGNYDLALEYYSKSKTNLGLTKTVGYATILNNIG
                     MVYYDQGNFDLALEHYSKSKTMQEGLGLTKTNLYATTLNNIGMVYNDQGNYDLALEHY
                     SKSKTIREKLGLTKTDGYATNLNNIGLVNYRQGAYCKAVEWFKKAVIIREQIGVPGNR
                     KNLDIAQKKCNESKRR"
     gene            1272..1919
                     /locus_tag="PLF66_18565"
     CDS             1272..1919
                     /locus_tag="PLF66_18565"
                     /inference="COORDINATES: protein motif:HMM:NF012297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004623 - phospholipase A2 activity
                     [Evidence IEA];
                     GO_process: GO:0006644 - phospholipid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0050482 - arachidonic acid secretion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A2 family protein"
                     /protein_id="HMZ66100.1"
                     /translation="MKKFILVIFVLELGFGCSTPSVTVKKVKTLEVNEINTKNLEYHT
                     IYEDHIDTLMRECFSELKKCKENKEKVEELDAYFKTEKYKSLSLLNRFYKKNYGYQIA
                     PPSRVISSLDNWLLGYEKLKYGCYCGTGSRNCPPIDQFDKLCKDHDDCYSLLKRPSEK
                     ETVKCDPVFRDETEKISKLPYIKDDKKRNDMIDSAAGASALFWLKTKFGFEIYKE"
     gene            2128..3246
                     /locus_tag="PLF66_18570"
     CDS             2128..3246
                     /locus_tag="PLF66_18570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66101.1"
                     /translation="MPLILTITILLLSCASEQKRTKLDSAYVEVTAFGSSLDDADTEA
                     KLQIVERGIGVLVEGGSAVADGQLQYSVVSTFSQGYVTDYQRISNEKQGSGYLVKAKG
                     KPNLKAIGDALEQRRREIGNPKMLILMTENMFGKNSKAGSTKSEYIFQASLKKDGFQF
                     DDKETVMKILAREKGLEVGAYGNPKAEELAQKIAVELNTDILVIGEAILKNAGEIRKG
                     TGLFSIQTDVKYKLVDVGSGAIIATATDTAIASDVDQELGATEALNQVTEKLTPKIKE
                     QIGKQWQAGSTTRISFKGIKLGEFLSSDITDTIKKIRGVNGVDDRGNTGIGPTIEVKC
                     YCGRFELVKRISKRSFEMDYKLVMKEKKGNLIVFEVKK"
     gene            3305..3556
                     /locus_tag="PLF66_18575"
     CDS             3305..3556
                     /locus_tag="PLF66_18575"
                     /inference="COORDINATES: protein motif:HMM:NF016167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HMZ66102.1"
                     /translation="MEVVTKQSFELIASNLEILGGKPCIKGTRISVDMILEWLATGAS
                     IQDICKTYPHLNEKSIIQAILYAATRLKNESFFELRKTA"
     gene            4267..4584
                     /locus_tag="PLF66_18580"
     CDS             4267..4584
                     /locus_tag="PLF66_18580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66103.1"
                     /translation="MKQFIKSSLLILLTVFIAMLSKVDSSKSTFVGKNYSFSNSVSNV
                     YTETLEEAFDSEENDLNLLDLSFLFSATTPFLISTVFLFSIFYTLRKPFLFRFSNIPP
                     PSL"
     gene            4696..6309
                     /locus_tag="PLF66_18585"
     CDS             4696..6309
                     /locus_tag="PLF66_18585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008590119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HMZ66104.1"
                     /translation="MNAIHILRVISIVTLSNIVFTYCLSGPALDLAPKYNLPKFVVPD
                     SWNGSGPFIKAKPSDDEIREEWWKLFNDPVLNQLEEEAMTANADLQASAERFLQARDM
                     MLKVRSKLIPNIGIGVVASNNKQSEQALFKGTFDPTYDKNITMGSLATWEPDFWSAIR
                     NETWAKIYEAESVAAEYALAKLSLQSELATNYFTLRGLEAQDATYRLSIEYYKQSLKI
                     VESRFKGGIAPQLDIFRAQYLLSSTEAKRYDIQSQRKVIENAIAVLINRSPSTFKIEP
                     QDELMTADFKIPAHLPANLLERRPDVAAMERKMARANREIGIAKAAFFPNVSFNLKGG
                     FEGGTNLFSLSNGFWSYGAMTSVPLFQGGYRRAQLQQAWSQYRETEDRYRSTVLNAFR
                     EVENGLAQTYFYSLEVEKLNAAVETAVKTQDMTTQLYTTGLSNSLELLYAQLSTLTSK
                     IDAIQVKTNLLNASVSLIRSLGGGWTRKKLPKEDDIQPMDIFEPVDFTNPKQIKGIEV
                     NSKDNTKYNDFTKPSNPAKAASEDNSKGK"
     gene            6356..9634
                     /locus_tag="PLF66_18590"
     CDS             6356..9634
                     /locus_tag="PLF66_18590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008597083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HMZ66105.1"
                     /translation="MNEIVRIALKRPYTFVVFAILILIFGIQTILKSPTDVFPAIKIP
                     VVSVVWVYQGMLPEDVSGRITYMFERALTTTVEGIRNIDSRSYYGSSIINIELQPDTN
                     LPGAEAEITAISQTVVKSLPPDISPPMVMRLEASMVPVAMLQVTSDNMTPAELYNLAF
                     MRIRSLLVTIPGAIIPQPYGGTPMQLLVSLDKQKLLARNLSPMDVYKAFNQQSIVLPA
                     GDQKIGKTDWMVMTNAMPIEVKDFNNVPIKTMGNSTIYMRDVADVSLSGPPQMNSVLV
                     DGKQSVVIVVMKSGDASTLDVVDGIKKLMPRIKEISPDGVQLKILNDASIFVNDSIEN
                     VVHEMVLASALTGLVVLLFLGSWRATTIIATSIPLSLLCSIITLHLMEESINVMTLGG
                     LALAVGILVDDATVMIENIDTHLEMGKPLEEAIIDAANEIVVPTFVATLAIVIVWLPL
                     FQLSGVSGWLFMPMAKAVMFAMIASFILSRTLVPTMAKYMLEAHHTHGEGHESEHSHS
                     ALPEPLKETHEPTKFDLYKEKVLTVIQIENKVNFLKKIQKSFEHNFNHMREEYHFLLK
                     RVIANRKKFVTTFLIIATSSLLLFVFNGRDFFPEIKSGTLQMHMRAPLGTRLEVAGRI
                     ATLVSEDIKNKILPNKVENVLSNCGLPVVSPHNMAFIPTPTIGSQDCDLTISLIDEES
                     PVWDYRKILRKELSERYPGTIFTFQPADLTSVILNFGSPSPIDIQINGLDLQENFKFA
                     QELQGKLKEVTGSSDVVIQQTMNTPTLMVEGNRSLGLNVNLPLKALAENMLLATSGSQ
                     QIDQEYWLDRKTGISYQVNIFVPQPQMKKTEDLLTVPINTGELDTTGDTKVQLLGNLA
                     HIKPTGTPGLITHHNLLSLIDIYVSAEGRDIGGVLSDVEKVIEKMKDKLPRGSAIEIH
                     GQAETMKTAYIELIGGLVLAIVLVYLLIVVNFQSWTDPFIIITALPGAIAGIAWALFL
                     TRTYISVPALTGAIMCMGTATANSILVVSYAREKLQEHGDAIRAATEAGYARIRPVIM
                     TASAMIIGMVPMSMSNSQNAPLGRAVIGGLTVATFATLFFVPCVYAIIYNKRSNNERS
                     ENHV"
     gene            9627..10829
                     /locus_tag="PLF66_18595"
     CDS             9627..10829
                     /locus_tag="PLF66_18595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016544676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HMZ66106.1"
                     /translation="MFKQIQTNKIYLASLVILSLVSFHCHKGANGKQNSKPDVSMVTI
                     VQAKAPEPVETITLPGNLKAWYEAPLYAQVSGYVKMWYKDYGAEVEKGDLLAEVQAPT
                     LDAEYAQARADLDSQDARFQLADVTANRYLALRNANAVSEQSISVAVADKNAEKAKLH
                     ASVQNLKKYGALIGFKKIVAPFKGIVTERNINVGDYVNKDGNLTDTRTKANLFTVSDI
                     HKLRLFVSVPGAYAYLLKNGLTADVKVAQVPNRIFKANFLTIANGFDPNTQTVIAQFT
                     IDNKDHTLWPGSYATVNLSAPVKKNVYTIPTSALVFNENGTQVAVVTNDNKVHFKAIR
                     VNKITDAFVEVLEGVTEEDRIINNPKASFLERDLVKVVTQDEKKLENEEKLSQIDSKN
                     TKEEDKHD"
     gene            10822..11976
                     /locus_tag="PLF66_18600"
     CDS             10822..11976
                     /locus_tag="PLF66_18600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016544676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HMZ66107.1"
                     /translation="MIKHITKKDAFKFVGIGFILFILFEVAIRFYSFHQLKKETLEAA
                     IPTVAIIHPAPPNPIETITLPGTLKAWYEAPIYAQVSGYVKMWYKDYGAEVEKGDLLA
                     EVQTPALDAEFAQAKADLQSQDAKYQLADVTANRYLSLQKSHAVSDQSISVAVADKNA
                     EKAKLQAAMKNVHKYEALINFKRIVAPFKGIVTQRNINVGDYVNKDGNLSDNKPITNL
                     FTVSDIHKMRLFVSVPGSFAYLLKSGLTADVTVAQFAQKTFKANFITIAKGFDPGTQT
                     VLAEFSIDNEDRTLWPGSYATVNMTAPVKKDLLTIPSSALVFNEKGTQVAVVTKENKI
                     HFKPIQVSKIMDTIVEILDGVTMEDSIINNPRTSFLEGDTVRIVAPREGY"
     gene            12349..12942
                     /locus_tag="PLF66_18605"
     CDS             12349..12942
                     /locus_tag="PLF66_18605"
                     /inference="COORDINATES: protein motif:HMM:NF040144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5683 domain-containing protein"
                     /protein_id="HMZ66108.1"
                     /translation="MIFKSILKVVILLSLLYTPLFSEAKNSPELKPDVYYAGWSSLLP
                     GLGQVKKDRLYSGIFFFSLFAASAINWQKKLQEFNQAENDYKSKIVFINLISISSPQS
                     SSSANISSGLFSYILLGQQAFRPYQIASDHSNLALSYVVVIYFVQIMHAFFCSPGDSF
                     QVKNESKQSYLNFNISPTRNQFGQTGTNVDLSYTWRF"
     gene            12942..13862
                     /locus_tag="PLF66_18610"
     CDS             12942..13862
                     /locus_tag="PLF66_18610"
                     /inference="COORDINATES: protein motif:HMM:NF019224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1554 domain-containing protein"
                     /protein_id="HMZ66109.1"
                     /translation="MKRLLLICLFLIGCTRAQKSPLDPSSSNGRISFLLRTIINSSSS
                     STSSSSLTPTLSYNPSSYVSYIGESVSIIANSSNVSSCTVSPSLPSGLTLSGTTCNIT
                     GTPTSSSSNATYTITAKNGNNSVSSTLSLTISPIYRTYITSVPVAANITLTTADSTCN
                     SNGNKPNSSTYKAMLVTSTRRACSSSYCNTSGSSENLDWVFKANSAYFQSNGTTLVGK
                     TNSSGIFTSSFNSYSISLNSSFSNSSFNYWTGLNTDYTTNSSNNCSNWTSNAGGSNGT
                     TGFSTSTIENAFSFFTFACSNTNYLLCIEQ"
     gene            complement(14037..14948)
                     /gene="rssA"
                     /locus_tag="PLF66_18615"
     CDS             complement(14037..14948)
                     /gene="rssA"
                     /locus_tag="PLF66_18615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010573502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004622 - lysophospholipase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HMZ66110.1"
                     /translation="MIVKNGKNLKLGLALGSGSSRGWAHIGVIRRLEELGIKPDVVVG
                     TSVGSLVGAAYCAGKLTDLESWLVSLKWQDVVSFFDLSFAGGLIKGNKLFKFFESHFQ
                     DTKIEDLQIPFGAVATDLETGQEIWMRSESLLSAVRASISLPGIFSPARRDNRWLVDG
                     GMVNPVPVSLCRAMGAEFIIAVDLNAYLLDPIQKLEQEKKEVKEEETKSKWSFMDSFI
                     KKSSPKEETESIKEETEGKPSMMEVINRSINIMEVKITRSRMAGDPADITLAPRLSHI
                     NMLEFHRASECIEEGKRVVKLIEDFLK"
     gene            complement(14992..16299)
                     /locus_tag="PLF66_18620"
     CDS             complement(14992..16299)
                     /locus_tag="PLF66_18620"
                     /inference="COORDINATES: protein motif:HMM:NF014697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00366 family protein"
                     /protein_id="HMZ66111.1"
                     /translation="MLQRLANFLNHLLARFLPDPFIFAVIISLFIYLVGILTGSSFLQ
                     MTYSFGKGFFSLNAFTMQMLFILVSGHILANTAVSHHFLNFFLKRIETPRSAIVLTII
                     VSILVSWLNWGFGLIAGAIFAKEVAKKVKEVDYRLLIASAYSGFVVWHGGLSGSIPLT
                     IATEKHFAESSMGIVPVSETLFSPLNLFILITLLVGLPIINLWMLPKKEDRVFYEEKE
                     EVKMNPITVDPSLANRLENSRILSILIFILGFSYIFLYFLQKGFQLNLDIVNFCFLFF
                     GILLHKTPIRFLKSAENAVRGTSGIILQFPFYAGIMGMMVDSGMAKSISQFFVLLADN
                     TVFPLFTFLSAGLINLFIPSGGGQWAVQGPIVLNAAKELGISYSKVAMAVAWGDAWTN
                     LIQPFWALPALAVAGLSIRDIMGFCIINLFFTGIVISGAFLLF"
     gene            complement(16280..17179)
                     /locus_tag="PLF66_18625"
     CDS             complement(16280..17179)
                     /locus_tag="PLF66_18625"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HMZ66112.1"
                     /translation="MKRVWVADLVLLLLNVMWGYCFILIKQLLSEIPPYFLLGTRFLL
                     AFFLLFPFQISAIPKMGKKELAWYFRCGIALGSGFCLATAGMTSTNPGKAGVIIGSLV
                     VFVPIIDFFWMKNPISISIRVGTLTTFLGLCFISLDGVGHITEMNLGDILLLLGAIAY
                     AFHVVIVAKTYSEIKTVNPMLLALVQIFTVGFFGLVISILFEKIPTVLSVFAIYGICF
                     LGILGSLIAYIVQMWAQKYAPAPHVGVILSTEAVFACIFSYFILGEAFTFFMWIGGIL
                     VLAGIMLTQEILPFGRRHVTKTR"
     gene            17270..18139
                     /locus_tag="PLF66_18630"
     CDS             17270..18139
                     /locus_tag="PLF66_18630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004420834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body protein"
                     /protein_id="HMZ66113.1"
                     /translation="MLRGIYTGASGMTVQQTRMDVIANNLANVDKTAFKRDTTLFKTF
                     PEMLIHRYNEDGVGKVPMGSFDTAPVVGKLGIGAEINEVYTRFEQGAVKKTDNSFDLM
                     IQDKPGAERPAFFSVMTNRGERLTRSGAFVLDTNGYLVTPQGFPLLGENGPIQVTRAN
                     FLVKENGEIFINADIGNHPREGLTSAEHNRFKNPVLLDKLKIRTVENPRHLDKEGDSF
                     YVDTPESGEPRPFRDELEGPEVLQGYLEASNVQVVQEMVEMIEVNRAYEANSKAMQTH
                     DSLLGRLINEVSR"
     gene            18217..18615
                     /locus_tag="PLF66_18635"
     CDS             18217..18615
                     /locus_tag="PLF66_18635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66114.1"
                     /translation="MKTYLKLIFALILFTNSFILFSEEKKSGDDFIKIWNLLWEKNIG
                     MGGLVWNDAIQYSKQKGLRLPTNSEFLSNIEFLDSLEDDSYENISKKKIFYATHGRYW
                     SSTELEKDPNQAWLIHVCIPQSECGIITKK"
     gene            complement(18863..19432)
                     /locus_tag="PLF66_18640"
     CDS             complement(18863..19432)
                     /locus_tag="PLF66_18640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66115.1"
                     /translation="MNLYDIYEVDPKEDNRGNYFILPLEFSAGMYFGIRFGFNFSEFV
                     DFIVGLVGFDPLSDDVAGIPEPIYLEENWEDLDVIFDQKLNDAITPIISITKSKYNNK
                     DSKVMNHCFLYNSCIQYLQNENINSEFEATCNRGNGLFKVNSKCFAKNVIGFCQFSNY
                     EKLYLYKDGFWLIADSETDCKNLNGKFVK"
     gene            complement(19911..20312)
                     /locus_tag="PLF66_18645"
     CDS             complement(19911..20312)
                     /locus_tag="PLF66_18645"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HMZ66116.1"
                     /translation="MYLIDTSVWIESLKKDSNLNIQRHFKVKEIFICLPVYQEILQGI
                     REDSVFWAVKKALDHSQFLEETLTKETFEEAISIYRQARKKGITIRSTVDCIIASIAI
                     RNHATIIHKDRDYSEISKFTALKEKNINTFL"
     gene            complement(20313..20543)
                     /locus_tag="PLF66_18650"
     CDS             complement(20313..20543)
                     /locus_tag="PLF66_18650"
                     /inference="COORDINATES: protein motif:HMM:NF021474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HMZ66117.1"
                     /translation="MKRTNLVLDENILEQTLRLSQKKTYSEAVMTAMKEFIRSNEFSK
                     IFDFQGTGIWSGNLSEMRQDKKINKTKTKKRK"
BASE COUNT         7149 a         3762 c         3613 g         6191 t
ORIGIN      
        1 atcaaagggc gaattcagat agctgaaatt aaatatacag gtccaccagc tcagagctgg
       61 attgtgtctg gaattcgaga tactgtgaca aatgatttag caaagattaa agaagtatct
      121 gctatttcta tcgaggatca aagagaagca ttcaagctga ttgcgttaaa acaaaaaaca
      181 ggtgtaaatg tagatgcctc catagaagcc gcaaagatat tagccgcaga ctatctctgt
      241 gtgggaaatt tacagaacac aggtaaaacg cttcgattga acatccgcct cttgaaatct
      301 cctgatttca gcgcagagca atctacaacg atagacggca cactcgatga aatatttact
      361 ttgcaagata aagtagtcga atcactgtta gccaacgtag atgcaaagat gacaccggaa
      421 gaaagactcg ttatagaaat ctttactcca aaaaataaaa atgcgtatga attgtattca
      481 cagggtttag aaattcagga tacaaatccg aagaaggctc ttgatttgtt tttacaggct
      541 ttaaaattgg aaccggatta tttggacgca ctgaatcgag ttgggaacca atatgattca
      601 tttaataact tttcggaagc actgaactac catgaaaaaa gaaggaaaat tctagaggaa
      661 aaaggcttaa gtaagcacga aagatattct gagacattaa ataatatggg tgcggtttat
      721 tattcacaag gcaactatga cttagccttg gagtattatt ctaaatcgaa aacaaacctt
      781 gggcttacta aaacggttgg ttatgcgact atcttgaata atattggaat ggtgtactat
      841 gatcaaggta actttgactt agccttggag cattattcta aatcgaagac aatgcaggaa
      901 ggacttgggc ttactaaaac taatctttat gcgactacct tgaataatat tggaatggta
      961 tacaatgatc aaggcaacta tgacttagcc ttggagcatt attctaaatc gaaaacaatt
     1021 agagaaaaac ttgggctaac taaaactgat ggttatgcga ctaacttgaa taatattgga
     1081 ttggtcaatt atagacaagg agcctattgt aaagcagttg aatggtttaa aaaagcagtt
     1141 attatccgag agcagattgg tgttcctggt aatcgaaaaa atttagacat tgcacaaaaa
     1201 aaatgcaatg agagtaaaag aagataggag aggataatgg ataacagaat taatttagag
     1261 gatattaggg aatgaaaaag tttattttgg ttatttttgt tttggaatta ggatttggtt
     1321 gcagcacacc atctgttaca gttaaaaaag ttaaaacatt agaggtcaat gagataaata
     1381 caaagaatct tgaatatcac acaatatacg aggatcatat tgatacttta atgcgagaat
     1441 gcttttctga gcttaaaaaa tgcaaagaaa ataaggaaaa agtagaggag cttgatgcat
     1501 attttaaaac tgagaaatat aaatcacttt ccctactaaa tcgtttttat aaaaaaaatt
     1561 atggctatca aattgctcca ccttctagag ttattagtag tttggataat tggcttttag
     1621 gttatgaaaa attaaaatat ggatgttatt gcggtactgg ttctcgtaat tgcccaccaa
     1681 tagatcagtt tgataaactg tgcaaagatc atgacgattg ttattcatta ctgaaacgac
     1741 cctcagaaaa ggaaactgta aagtgtgatc cagtatttcg tgatgaaact gaaaaaatat
     1801 caaaacttcc ttatataaag gatgataaaa aaagaaatga tatgattgat agtgcagctg
     1861 gtgcaagtgc tttattctgg ttaaaaacta aatttggatt cgaaatttat aaagaatagt
     1921 tcaaaaattt agcagagtga caaaagaaaa attaaatttt cctatttaca ttttatatat
     1981 acaaatatta cgcataaatt tatatctaaa ttatttaaaa ttaaatacag gataaaatcc
     2041 acaaggcgaa aaaatttgga ttattcacaa gaagggtttt actttcttac catctgagca
     2101 acagcacacg taaaggcaat tcacgaattg cctctaattc taacaatcac aatcctcctc
     2161 ctatcctgcg cgtccgagca gaagagaaca aaactagaca gcgcctacgt agaagtaacc
     2221 gcgtttggct cttctctgga tgatgcggat accgaagcaa agctacaaat tgtagagaga
     2281 ggcataggcg tgttagtcga aggaggaagt gcagtagcag atggacagtt gcaatacagt
     2341 gtggtttcta cgttttcaca agggtatgta acggactatc agagaatttc caatgagaaa
     2401 caaggaagcg gttatttggt gaaggctaag ggcaaaccaa acttaaaagc aataggcgat
     2461 gcactggagc aaagaagaag agaaatcgga aatcctaaaa tgctgatttt gatgactgaa
     2521 aatatgtttg gaaaaaactc gaaagcgggc tctacgaaaa gtgaatatat ctttcaagca
     2581 tctttgaaaa aagatggatt ccagtttgac gacaaagaaa cagtgatgaa aattctagcc
     2641 cgcgaaaaag gactcgaagt aggagcttat ggaaatccga aagcagaaga gttagcacaa
     2701 aaaattgcag tcgaattaaa tacggatata cttgtaatcg gagaagcgat tcttaagaat
     2761 gctggtgaaa ttcgaaaagg cacaggactt ttctctattc agactgacgt taaatacaaa
     2821 cttgtagacg ttggctctgg tgccattatt gccactgcaa ccgatacagc tatagcgtct
     2881 gacgtagacc aagagcttgg agcaacggaa gcactcaacc aagtaacaga aaagcttact
     2941 cctaaaatca aagaacagat tggcaaacaa tggcaagccg gtagcacaac tcgcatttcc
     3001 ttcaaaggaa tcaaactcgg agaatttcta tccagtgaca tcaccgacac aatcaaaaaa
     3061 attcgaggag tcaatggagt ggacgataga ggaaacaccg gaataggtcc taccattgag
     3121 gtgaaatgct actgcggaag atttgaacta gtaaaacgta tttccaaaag aagcttcgag
     3181 atggattaca agttagtgat gaaagaaaaa aaaggaaatt taattgtgtt tgaagtaaag
     3241 aagtgagagt taaaaaactg aaaaattact tgtaatattt gtgtattttt tacaattctt
     3301 tattatggaa gtagtaacga aacaatcttt tgaattaatc gcttcaaacc ttgaaattct
     3361 tggcggaaag ccttgtatca agggaacaag aatcagtgta gatatgattc tagaatggct
     3421 tgccacaggt gcatctattc aagatatttg taaaacttat ccgcacttga atgaaaaatc
     3481 aataatccaa gcaattcttt atgctgccac cagattaaaa aatgaatctt tttttgagtt
     3541 aagaaaaaca gcttaaaaaa tgattctaaa agatttcaag tttcttactg atgaaaatat
     3601 tcatcctgaa gttgtattgt atttaaggaa atccggattt gacgtcaaag atgtaaaaga
     3661 ataaggatta tctggaaatt cagatttatt tttactgaag cttgcccatc aagaatgtag
     3721 agtagtatta acacatgata gtgattttgg aaaaataatc tacaccgaaa cgattgactt
     3781 tactggaatt gtttatttac gtccaggtca ttttctatcc gaatttacga tctctacttt
     3841 agatagtctg ttgagattta atttagaaaa tattaatgtt ccatttattc ttgtaggaga
     3901 gaatacagga gaaacaatca gagttcgagc aagaaatgta atattatcct aaaattaaaa
     3961 atattccgag aaaggaaaca taaccgatga gtttttaggc gactttcctt ccgtttcaaa
     4021 atagaaggct atcaatacta ttttaaatcc aatatatata aacccaaaac cacttcacag
     4081 caatacttct atggtatttc attcataggc tcgcattttc acctaatatc tcaatggatt
     4141 cttctttcac atgactttaa ttttatgaat tttaatatat gaattttttt tcatgtttcg
     4201 tagaaaaagt tgttgttttt ttaagagaaa atacagacta ataataaagg gaaatagaaa
     4261 atgaatatga aacagtttat caaatcatct ctgttgatac ttcttacagt attcattgct
     4321 atgttaagta aagttgattc ttcaaaatca acttttgttg gaaaaaatta ttctttttct
     4381 aactcagtct ctaatgttta tacagagact ctagaagaag catttgattc agaagaaaat
     4441 gatctaaatc ttttagatct cagtttccta ttttcagcta ccactccttt tctaatctcc
     4501 actgtatttt tattttcaat cttttataca cttcgaaaac catttctatt tagattctca
     4561 aacattcccc ctccatctct ataactccca acattcgttc catttcttaa atagaaatgg
     4621 aaatcaatag agagaatttt ctctcttagg gaatataaat ccgaaaccgg ataaaagaaa
     4681 ttaaggagta atagtatgaa tgctattcat atacttagag tcatctctat agtcactctt
     4741 tcgaatatcg tatttactta ttgtttgagc ggacctgcac ttgacttagc acccaaatac
     4801 aaccttccca aatttgtagt tcctgattcc tggaatggtt ctggtccttt catcaaggca
     4861 aagccttcag atgatgagat aagagaagaa tggtggaaac tatttaatga tccagtttta
     4921 aaccaattag aagaagaggc aatgacagca aatgcagatc tacaagcatc agccgaaaga
     4981 tttttacaag caagagacat gatgttaaaa gttagatcga aattaatccc aaacattggg
     5041 ataggcgttg tagcctccaa taataaacaa tcagaacaag cgctttttaa aggaaccttt
     5101 gatcctacat acgataaaaa tattacaatg ggaagcctcg ccacttggga acctgacttt
     5161 tggtcagcga ttcgaaatga aacatgggca aagatttatg aagcagaatc tgttgctgct
     5221 gaatatgcac ttgcaaaact aagtctacaa tctgagctgg ctacaaatta ttttacactg
     5281 cgaggattag aagcacagga tgcaacctat cgtctatcga tcgaatatta taagcagtct
     5341 cttaaaattg tagaaagcag atttaaaggg ggaattgcac ctcaacttga tatttttcga
     5401 gcacaatacc tactctctag cacagaggca aaacgatatg acattcaaag tcaacgcaaa
     5461 gtaatagaaa atgcaatcgc agttttgatc aatagatccc catctacttt taaaatagaa
     5521 ccacaagatg agctcatgac tgctgatttc aaaattcctg ctcatcttcc cgctaatctt
     5581 ttagaaagaa gaccagacgt agccgcaatg gaaaggaaaa tggcaagagc caatcgagaa
     5641 atcggtattg caaaggctgc attctttcct aacgtttcct ttaatctaaa aggtggtttt
     5701 gagggaggga cgaatttatt tagcttatca aatggattct ggtcctatgg tgcaatgaca
     5761 tcagttcctc tctttcaagg gggatatcgt agagcacaac tccaacaagc ttggtcccaa
     5821 tatagagaaa ctgaagatcg ttatcgctcc actgttttaa atgcatttag agaagtggaa
     5881 aatggtttag cgcaaaccta tttttattct ctagaagtag aaaaattaaa tgcggcagtt
     5941 gaaacagctg taaaaacaca agatatgaca actcagctct atactacagg gcttagcaat
     6001 agcttagagt tactctatgc gcaattaagc acacttactt ctaaaattga tgcaattcaa
     6061 gtaaagacca atttactaaa tgcatctgtt tctcttattc gttcgttagg tggaggttgg
     6121 actcgtaaga aactcccaaa agaggatgat atacaaccta tggatatttt tgaaccagtg
     6181 gattttacaa atccaaaaca aatcaaggga atcgaagtta attcaaaaga taatacaaaa
     6241 tacaatgatt ttacaaaacc ttcgaatcca gctaaagcag ccagtgaaga taattccaaa
     6301 ggaaagtagg aataaaacat gaaaaccatt cgattcaaat caaaggagca aagccatgaa
     6361 tgaaatcgtt cgtatcgcac ttaaaagacc ttataccttc gttgtattcg ccattttaat
     6421 tttaattttt ggaatccaaa ccattctaaa gtcaccaact gacgtattcc ctgcgattaa
     6481 aataccagta gtatccgttg tgtgggttta ccaaggaatg cttccagaag atgtttctgg
     6541 aagaattacc tacatgttcg aaagagcttt gactacaacg gtagaaggga ttagaaacat
     6601 cgatagtcgc tcttactatg gaagtagtat tatcaacatc gagttacagc cggatacaaa
     6661 cttacccggg gcagaggcag agattacagc tatctcacag acagttgtga aatctttgcc
     6721 tcctgatatt tctcccccca tggtgatgcg cctagaagct tccatggttc ctgttgcaat
     6781 gttacaagta acgtcagaca acatgactcc agcagaactc tacaacctag cctttatgag
     6841 aattcgttct ctacttgtga ctattccagg cgccattatt ccgcaaccgt atggaggaac
     6901 tccaatgcag ttgcttgtat ctctagataa acagaagcta ctcgctcgta acctctcccc
     6961 gatggatgta tacaaagctt tcaatcagca aagtatcgtc ttgcccgctg gagatcaaaa
     7021 gattggtaaa acagattgga tggtaatgac taacgccatg ccaatcgaag taaaagattt
     7081 taataatgtc ccaattaaga ctatgggaaa tagcacaatc tatatgagag atgtggcaga
     7141 cgtttccctt tcaggtcctc cgcaaatgaa ctcggttctt gtggatggaa aacaatctgt
     7201 agtgattgtt gtgatgaaga gtggggatgc ttctacactc gatgtcgtgg atggaattaa
     7261 aaaattaatg ccgcgtataa aagagatttc tcccgatggg gttcaattaa agattttaaa
     7321 tgatgcatcg attttcgtaa atgactcgat tgaaaacgtt gtgcatgaaa tggttctcgc
     7381 atctgctttg actggacttg tagttttact ttttcttggt tcgtggagag caactacaat
     7441 cattgcaact tccatcccac tttctcttct ttgctctatt attacacttc atctcatgga
     7501 ggagtctatc aacgtaatga cgttaggagg tcttgctctt gcagtaggta tcttagtcga
     7561 tgatgctacc gtgatgattg agaacattga cacacacttg gaaatgggaa aacctttgga
     7621 agaagcaatc atagatgcag caaatgaaat cgtagttcct accttcgttg ctactcttgc
     7681 gatcgtaatt gtatggcttc ctctctttca attgagcgga gtttcaggat ggctctttat
     7741 gcctatggcg aaagcagtta tgtttgcaat gattgcttcc ttcattcttt ctagaacgtt
     7801 agtgcctaca atggctaagt atatgctaga agcacatcat actcacggag agggacatga
     7861 atcagaacat tcacattctg ccctcccaga gcctttgaaa gaaacacatg agcctactaa
     7921 attcgatctt tataaggaaa aagttttaac ggtaattcaa attgagaata aagtcaattt
     7981 cttaaaaaag attcagaaat cttttgaaca taatttcaac catatgagag aggaatatca
     8041 ctttctatta aaacgagtca ttgcaaatag aaagaaattt gttaccactt ttctaatcat
     8101 agcaacctcc tctcttcttc tctttgtttt taacggaaga gatttctttc cagaaatcaa
     8161 atcaggaact ttgcaaatgc atatgagagc accacttgga acaaggcttg aagtagcagg
     8221 aagaattgca actttagtat cagaggatat taaaaacaaa attctaccca ataaagtaga
     8281 aaacgtattg agtaactgtg gtcttcctgt tgtgagtccc cacaatatgg catttattcc
     8341 tacaccgact atcggttcac aagattgtga tctcactatt tcactgattg atgaagaatc
     8401 ccctgtctgg gattatagaa agattctaag gaaagaatta agtgagagat acccaggaac
     8461 tatatttaca ttccaaccgg cagatctgac ctctgtgatt ttaaattttg gttccccttc
     8521 tcctattgat attcaaatca atggtcttga cttacaggaa aattttaaat ttgcccaaga
     8581 attacaaggg aaactaaaag aagtaactgg ctctagtgat gtggtaattc aacaaacaat
     8641 gaatactcca acgcttatgg tggagggaaa tcgaagtttg ggtttaaatg taaatcttcc
     8701 tctgaaagcg ttagccgaaa atatgcttct agcaacttcg ggaagccaac agatcgacca
     8761 agagtattgg ctagatcgaa aaacaggaat ttcttaccag gtaaatatct ttgttccaca
     8821 gccacaaatg aaaaagacag aggacttgct cactgttcca attaatacag gagagctgga
     8881 cacaactgga gatacaaagg ttcaacttct aggcaacctt gctcatatta aaccaacagg
     8941 aacacctggg cttattaccc accacaatct tttatctctc attgatatct atgtttccgc
     9001 agagggaaga gatattggag gagttttgtc agacgtagaa aaagtaatcg aaaagatgaa
     9061 agataagtta ccaaggggtt ctgcgataga aatacatgga caggcagaaa caatgaagac
     9121 agcctatatc gaactgatag gtggacttgt tttagcaatt gtgctagtgt atctactcat
     9181 tgtagttaat tttcaatctt ggacggatcc atttattatc attacagctt taccaggtgc
     9241 gatagctggc attgcttggg ctttatttct aactagaacc tatatatcgg ttcctgcgct
     9301 tacaggagcg atcatgtgta tgggaacagc taccgcaaac tctatcttag tagtttcata
     9361 tgctagagag aaattgcaag agcatggaga tgcaataaga gctgctactg aagcaggata
     9421 cgcaagaata agacctgtta tcatgacagc ttccgcgatg attattggta tggttcccat
     9481 gtctatgagt aattctcaaa acgccccttt gggtagggca gttatcggtg gactaacggt
     9541 tgctacattt gcaaccttat tctttgttcc ttgtgtttat gcaatcattt ataacaaacg
     9601 ttcaaacaat gaaaggagtg aaaaccatgt ttaaacaaat tcaaaccaac aaaatctatt
     9661 tagcaagctt agttatacta agcctagtta gcttccattg ccacaaaggg gcaaacggta
     9721 aacagaattc aaaacctgat gtttctatgg taacaattgt gcaagcaaaa gctccagaac
     9781 cagtagaaac gatcacgctt cctggaaatt tgaaagcatg gtatgaggca cctctttatg
     9841 cacaggtttc tggttatgtt aaaatgtggt ataaagacta tggggcagaa gtagaaaaag
     9901 gcgatctctt agcagaagtc caagccccta cactagatgc agagtatgca caagcaagag
     9961 ctgatttgga ttcacaagat gcaagatttc aattggctga tgtaacggct aatcgttatt
    10021 tggcgcttcg aaatgcaaat gctgtatcag aacagtccat ttctgtagca gtagcagata
    10081 aaaatgcaga aaaagcaaag ttgcatgctt ctgttcaaaa tctaaaaaaa tatggggcac
    10141 ttattggatt taaaaaaatt gtagctccct ttaaaggaat tgtaaccgaa agaaatataa
    10201 acgttggtga ctatgtaaat aaagatggaa atttgacaga tacaagaacg aaagcaaatc
    10261 tctttactgt atctgatatt cacaaattaa gactattcgt atctgttcca ggtgcttatg
    10321 cttatcttct aaaaaatggt cttacggcgg atgtaaaggt agcacaagtt ccgaacagaa
    10381 tcttcaaagc aaactttcta accattgcga atggatttga tcccaatact cagactgtaa
    10441 ttgctcaatt tactatcgat aataaagatc acactctttg gccgggttcc tatgcgactg
    10501 taaatctttc tgcacctgta aaaaagaatg tatatacaat tcctacgagt gctcttgtat
    10561 tcaatgaaaa tggaactcaa gtagctgttg tgactaacga caataaagtt cattttaaag
    10621 caattcgagt aaataaaatt acagatgctt tcgtagaagt tttagaaggt gtaacagaag
    10681 aagatcgtat tatcaataat ccgaaagcat cttttttaga aagggattta gttaaagtag
    10741 taacacaaga tgagaaaaaa ttagaaaatg aggaaaaact ttctcagata gattcaaaaa
    10801 atactaagga ggaagataaa catgattaag catataacaa aaaaagatgc ttttaaattc
    10861 gttggaatcg gatttattct gtttatttta tttgaagtag cgattcgatt ttatagcttt
    10921 caccaattaa aaaaagaaac tttggaggcg gcaattccaa cggttgcgat tatccatcca
    10981 gcccctccaa atcctattga aactatcact ctgccaggca ctttaaaagc ctggtatgaa
    11041 gctccaatct acgcccaagt ttcgggctat gtaaaaatgt ggtataaaga ttatggagcg
    11101 gaagtagaaa aaggagatct tttagctgaa gtgcaaaccc ctgcgttaga tgctgaattt
    11161 gcacaggcaa aggctgattt acagtcacaa gatgcaaaat accaactagc agatgtaacg
    11221 gctaatcgct atttgtctct acaaaaatca catgctgttt ctgatcagtc catttcggtt
    11281 gcagtagcag ataagaatgc agaaaaagca aaactacaag cagctatgaa aaatgttcac
    11341 aagtatgaag cgcttattaa ttttaagaga attgtagcac cctttaaagg aattgttaca
    11401 cagagaaata taaacgtagg ggattatgta aataaagatg gaaatttaag tgataataaa
    11461 ccaattacta atttatttac tgtttcagat attcataaaa tgcgtctttt tgtttctgtt
    11521 cctggctctt tcgcctatct tttaaaatct ggactcactg ctgatgtaac cgtggctcag
    11581 tttgctcaga aaacttttaa agcaaatttt ataacgattg cgaaaggatt tgatcccggc
    11641 acccaaacag ttcttgcaga gttttccatc gacaatgaag atagaactct ttggcctggt
    11701 tcttatgcaa ctgtaaacat gactgcacct gttaagaagg atttactcac aattccatcg
    11761 agtgcccttg tattcaatga aaaaggaaca caagttgcgg ttgtcacaaa ggaaaataaa
    11821 atccatttca aaccgattca ggtgagtaag attatggata ccattgttga aatcttagat
    11881 ggagtgacaa tggaagatag cattatcaat aatccaagaa cttctttttt agaaggagat
    11941 actgttcgaa ttgtagctcc cagagaggga tattaatatg aatttttaaa atttcaacaa
    12001 aaataataaa attcgttagg taggtggaac ttgagataca ctagataaga aataattgct
    12061 attacaatct gagtttgcat tacagctatt tactttagca tatttcagac aagaagatcc
    12121 accgcaatca atgtcggatt ctgtttcatt tttcaaaagg catatgcgag tgacacaatt
    12181 tgaattcaca gaggatttgt tcgaaacaga gtaagctcaa ataccgcatg cactatctat
    12241 atctttcgat tcttttacaa atacatttag cataaatcat ttctataagt tttatattcc
    12301 ttgaatattt ttattgacac catataatta caattcttag tttatactat gatttttaaa
    12361 tcaatcttaa aggtagtcat actactctct cttttatata cccccttgtt ttcagaggca
    12421 aagaattctc ctgaactcaa accagatgtt tattatgcag gctggtcatc actacttcct
    12481 ggactcggtc aggtgaaaaa ggacaggctt tattctggta tctttttctt ttctctgttt
    12541 gctgcaagtg ctatcaattg gcaaaagaaa ctacaagaat ttaatcaagc cgaaaatgat
    12601 tataaatcaa agatagtttt tattaatcta atttctatta gtagcccgca aagttctagt
    12661 agtgcaaaca taagttctgg tttgttttct tacatactcc taggacagca agcatttaga
    12721 ccataccaaa tagcgtcaga tcattccaat cttgctctct cctatgtggt tgttatctat
    12781 tttgtgcaaa ttatgcatgc ttttttttgt agcccaggag attcatttca agtgaaaaat
    12841 gaatccaaac aatcctattt gaattttaac atttctccta caagaaatca atttggacaa
    12901 acagggacaa atgtagattt gtcttatact tggaggtttt aatgaagcgg ctactcttga
    12961 tttgcctttt cctcatcgga tgcaccagag cccaaaaaag tccactagat ccatcttctt
    13021 caaatggaag aatcagtttc cttctccgaa ctataatcaa ttcatcgtca tcctctacat
    13081 cttcttcttc gcttacacca accttatctt ataacccatc ctcctatgtt tcctatattg
    13141 gagaatctgt ttccattatt gcaaactctt caaacgttag ttcttgcact gtaagcccga
    13201 gtcttccgtc tggtcttacc ttaagcggaa caacttgtaa tattacagga actccaacct
    13261 cttcttctag caatgcaact tacacaatca ctgcaaaaaa tggaaataat tccgtaagta
    13321 gcactctttc tctaaccatc agtccaattt acagaacata tataacttct gttcctgtag
    13381 cggcaaatat aacgctcact acagcagatt caacttgtaa ctcgaatgga aataagccca
    13441 actctagcac ttataaagca atgcttgtaa cctcaacacg aagggcttgt agttccagtt
    13501 attgcaatac aagtggaagt tctgaaaacc tagattgggt ttttaaagcg aattctgcat
    13561 atttccaatc caatgggaca acgttagttg gcaaaacgaa ttctagtgga atatttacct
    13621 ctagttttaa ctcttactct atttcattga attctagctt ttcgaactca agttttaatt
    13681 actggacagg tctaaacaca gattacacaa caaactcttc taataattgt tctaattgga
    13741 cttcaaatgc cggtggaagc aatggcacaa caggtttttc aacatccaca atagaaaatg
    13801 cctttagctt tttcactttt gcctgtagca acactaacta tcttctttgt attgagcagt
    13861 aaacagtgat ttaccgctct attttacatg agttcgatct accaaaaaaa tgaaacgatt
    13921 actaccgata taaactgagc ctgtcccaat gcgtaaacaa cgagaacgat aaataggaaa
    13981 ttttttaaac catctgtgtt cattcgtggt aaaatatttc ttgcattgaa ttcatgttat
    14041 ttcaaaaaat cctctatcaa cttaacaact ctttttcctt cttcaataca ttcactcgct
    14101 cgatgaaatt cgagcatatt gatatgactg agcctcggag ctaacgttat atcagcagga
    14161 tcacctgcca ttctacttct agtaattttt acttccatga tgttaatgct tcgattgatt
    14221 acttccatca tgctgggttt gccttctgtt tcctctttta tactttccgt ttcttctttt
    14281 ggagaggatt tttttataaa actatccata aatgaccatt tgcttttggt ctcttcttct
    14341 ttgacttctt ttttctcttg ctctaatttt tgaattggat caagtaaata agcgtttaaa
    14401 tccactgcaa taataaactc agctcccatc gcacgacaaa gagaaactgg aacaggattc
    14461 accattcctc catctacaag ccacctattg tctcttcttg ctggggaaaa tattcctgga
    14521 agagagatgg aggctctcac agcggataat agcgattcgc ttctcatcca aatttcttgc
    14581 cctgtttcta aatcagttgc aacagcacca aaaggaattt gtaagtcttc aatttttgta
    14641 tcttgaaaat gagattcgaa gaatttaaaa agtttatttc ccttgataag tccacccgca
    14701 aaagataaat caaagaagct aacaacatct tgccacttaa gactcactaa ccaactttct
    14761 agatctgtta attttccagc acaataagca gcacccacaa gagagccgac tgaggttccc
    14821 accactacat cgggttttat ccctaactct tctagtctgc gaatcactcc gatatgcgcc
    14881 caacctctag aagatccact tccgagcgcc aaacctaatt tcaaattctt tccattcttt
    14941 acaatcatag atcttagttt ttaaaagctt tattctttgg ccaattattt tttaaaaaag
    15001 aaggaatgca ccagagataa caatccctgt gaaaaataaa ttgataatac aaaatcccat
    15061 gatatcgcga atactaagac ctgcaacggc taacgcaggt aaagcccaaa agggttggat
    15121 taaatttgtc caagcatccc cccaggcaac agccattgca actttagagt aagaaattcc
    15181 tagctctttt gcggcattta atacaatcgg accttgcacc gcccactgcc ctccacccga
    15241 tggaataaat agattaataa gcccagcact taaaaaagta aagagaggaa aaacagtatt
    15301 atccgcaagc agaacaaaga attgagagat agactttgcc attccagaat ctaccatcat
    15361 tcccataatt cctgcataaa aaggaaattg aaggataatt cccgaagttc cacggactgc
    15421 attttctgca gattttaaaa atcgaatcgg tgttttatga agtaatattc caaaaaataa
    15481 aaagcaaaaa tttacaatgt ctaggttgag ttgaaatcct ttttgtaaaa aatataaaaa
    15541 aatataggaa aatccaagaa taaaaatcaa aatcgatagg attctagaat tttccaatcg
    15601 attggcaaga cttggatcaa ctgttatagg attcatcttt acctcttctt tctcttcata
    15661 aaaaactcga tcttcttttt tagggagcat ccagaggtta ataatgggta gccccacaag
    15721 aagtgtaatc aaaataaata gattcaaagg agaaaaaaga gtttctgata caggaacaat
    15781 tcccatcgag ctttcagcaa aatgcttttc tgttgcaatg gtaagtggaa tagaaccaga
    15841 aagtcctcca tgccaaacta caaaaccaga ataggcactt gcaatgagca aacgatagtc
    15901 cacctctttt actttctttg ccacttcctt cgcaaaaata gcacctgcaa ttaaaccaaa
    15961 tccccaattg agccaagaaa ctaaaattga tacaatgatt gtaagaacta tcgcacttct
    16021 aggcgtctca attcttttca aaaagaaatt taaaaagtga tgtgaaacag ccgtatttgc
    16081 aagaatatgc ccactgacta agataaagag catctgcatc gtaaaagcat ttaaactaaa
    16141 aaaacctttt ccgaaagaat aagtcatttg caaaaaagaa cttcctgtta aaattccaac
    16201 tagataaata aaaagactga tgatcacagc aaaaataaaa ggatctggta aaaaacgagc
    16261 tagtagatga tttaaaaaat tagcgagtct ttgtaacatg acgtctccca aatggtaaaa
    16321 tttcttgtgt aagcataatt ccagctaata ccagaatacc tccaatccac ataaaaaaag
    16381 taaaggcttc tcctaaaata aaataagaaa agatacaggc aaaaactgcc tctgtagaca
    16441 gaatcactcc cacatgaggg gctggtgcat atttttgtgc ccacatttgc acgatatacg
    16501 caataagact tcctagaatt cctaaaaaac aaattccata tatcgcaaat acacttaaaa
    16561 cagtaggaat tttttcaaac aaaatactaa ttaccaaacc aaaaaatcca actgtaaaaa
    16621 tttgcacgag agcaagtagc attggattta cagtctttat ttcagaataa gtctttgcaa
    16681 ctatcaccac atgaaaagca taggcaatgg ctccaagcaa aagtagaata tcgcctagat
    16741 tcatctctgt tatatgacct actccatcca gagaaataaa gcaaagtccc agaaatgttg
    16801 ttagtgtccc gactctgatg gagatagaaa ttggattttt catccaaaaa aaatcgatga
    16861 ttgggacaaa taccacgagt gaaccaatga ttacacccgc cttacctgga tttgtgcttg
    16921 tcattccagc tgttgctaga caaaagccag agcctagtgc aattccacaa cgaaaatacc
    16981 aagcaagttc tttttttccc attttaggaa tggcactgat ttgaaaggga aaaagtaaaa
    17041 aaaatgctaa caaaaatcgg gttcctagta aaaagtaagg cggaatctca gaaagcagtt
    17101 gtttgattaa aatgaaacag tacccccaca tcacattgag gagtaataaa accaaatcag
    17161 ccacccaaac acgtttcatg acagctactt tttgtgaatt gtccgtatta gaaacaaatt
    17221 tttacttaaa ttttttccta gctgttcgat agagagtata gaggtaatta tgctcagagg
    17281 aatatacaca ggcgcaagtg ggatgacagt tcaacaaact agaatggatg tgattgctaa
    17341 taatctagca aacgtagata aaacagcatt caaacgagac actaccctct ttaaaacatt
    17401 tcctgaaatg ctcatccatc gctacaacga agatggtgtt ggcaaagttc caatgggctc
    17461 ttttgataca gctcctgtgg tgggaaaatt aggaatcggt gctgaaatca atgaagtata
    17521 cacaaggttc gaacaaggtg ctgtgaaaaa aacggataat tcctttgatc taatgatcca
    17581 agacaaacca ggcgcagaaa gacctgcttt tttctctgtg atgacaaatc ggggagaaag
    17641 attaactcgc agtggagctt ttgtcttaga cacaaatgga tatttagtca caccacaagg
    17701 cttcccgttg ttaggtgaaa atggtcctat tcaagtgaca agagcaaact ttctagtaaa
    17761 agaaaatggg gaaattttta tcaacgctga tataggaaat catccaagag aaggtcttac
    17821 cagtgcagaa cacaatcgtt ttaaaaatcc tgtcctactc gataaactaa aaatcagaac
    17881 ggttgaaaat ccaagacacc tagacaaaga aggagactct ttttatgtag acactcctga
    17941 gtctggagaa ccaagacctt ttcgcgatga attagaaggt cctgaagtat tacaaggcta
    18001 cctagaagcg tctaacgtgc aagtcgtcca agaaatggta gaaatgatcg aagtcaatcg
    18061 agcctatgaa gcaaattcga aagcaatgca gacacacgat tccttacttg gtcgacttat
    18121 caacgaagta agtagataaa tcataacgtt tcgaaagtct tcaatattct ccttatatag
    18181 ttaacaaaac tcagatatat gtacgcgaga ataaaaatga agacgtattt aaaactaatt
    18241 tttgctttaa ttctttttac taattcattt atcctttttt ctgaagaaaa aaaatctggt
    18301 gatgatttca taaaaatttg gaatctgctt tgggaaaaga atattggaat gggtgggctt
    18361 gtttggaatg atgcaattca atatagcaag caaaagggat tgaggcttcc taccaattca
    18421 gaatttcttt ctaatataga attcttagat tcattggaag atgactctta tgaaaatata
    18481 tcaaagaaaa aaatttttta cgcaactcat ggcaggtatt ggtcatctac agaacttgag
    18541 aaagatccaa accaagcatg gcttatccat gtttgcattc ctcagtctga atgtgggatt
    18601 ataaccaaaa aatgacaacc tacatttcca attaccaagc tacgccttat gaaaattttc
    18661 ttctatcttt tataaataaa ggcatgagtt atgaaactca taacacattt cagcttagga
    18721 atttctggat aggatattcc acttcattgt aaaaaaaatc cgaaaatcaa ttttactttt
    18781 ctctttcttc gtgaaccttc gtgctcttcg tggttgaaaa ttaatgatgc ggcaaatatg
    18841 tattagccag aaattattct acttacttaa caaatttacc atttaggttt ttgcaatctg
    18901 tttctgaatc agcaataagc caaaatccat ctttatataa gtataacttc tcataattcg
    18961 aaaattgaca aaacccaatt acatttttcg caaagcattt agaattaacc ttaaataatc
    19021 catttcctct attgcaagta gcttcaaact cagaattaat attttcattc tgcaaatatt
    19081 gaatgcaaga attgtataaa aagcaatgat tcattacttt tgaatctttg ttattgtatt
    19141 tacttttagt gattgaaata atcggagtaa ttgcatcatt caacttttga tcgaatataa
    19201 cgtccaaatc ctcccaattt tcttccaaat atataggctc tggaatacca gcaacatcat
    19261 cacttaacgg atcaaaacca acaagaccaa ctataaaatc aacgaactcg gaaaaattaa
    19321 atccaaatct tattccgaaa tacattccag cagaaaattc caaaggtaaa ataaaataat
    19381 ttcctctatt atcttctttc gggtctacct cataaatatc atataaattc atgcctcttc
    19441 ccttaccatt cggaggacta agatagattg tgaatgaaac taacttcttt ttatttcgat
    19501 tggtaactat aagaggttta tgataattac tgttgtaaaa taaataagaa ttaccataag
    19561 catattttgt tttattgaaa acgccaagtt catcagaaca ctttttttta aaatacagga
    19621 atgattcaat gattcggatt tgattgtatt caaaataaaa tatttaccga cagttttata
    19681 aaacctaacc gttccgtaac gcattccaat tcctttgcca ttagccgcat gttggattcc
    19741 tagtccaaaa gaatcaacaa agatggatgc cccatatata tccttctcta aaccgatatg
    19801 aataatatca ctagcatccc ttgttcgatc atacagataa tttctaacac tagaacaaaa
    19861 ctatagttgc aggattagaa ttgtgaatag aaaccgattc atattagaat ctaaagaaag
    19921 gtatttatat ttttttcttt caatgctgta aatttactaa tctcagaata gtctctatcc
    19981 ttatgaatta tagttgcatg atttctaata gcgatggatg caattataca atccactgtg
    20041 gaccgaattg taattccttt ttttcgagct tgacgataaa ttgatatcgc ttcttcaaaa
    20101 gtttccttcg ttaaagtctc ttctaaaaat tgtgagtgat ccaatgcctt ttttactgcc
    20161 caaaaaactg aatcttcgcg aatcccttgt aaaatttctt ggtaaacagg taggcaaata
    20221 aatatttctt taactttaaa atgtctttga atattcaaat tagaatcctt ttttaaagat
    20281 tcaatccaaa cagaagtatc gattaaatac atttattttc gcttcttagt ttttgtttta
    20341 tttatttttt tatcctgtct catctcagaa agatttccag accaaatgcc agttccttga
    20401 aaatcaaata tcttggaaaa ttcatttgaa cgaatgaatt ccttcatagc agtcattaca
    20461 gcttctgaat atgtcttttt ctgagaaaga cgaagtgtct gctctaatat gttctcatct
    20521 aacacaagat ttgtgcgttt catacacata ctatgattta cgataaattc taagtcaaga
    20581 aataaataac tctctatatt ctattcataa atttcattat ccctagtctt cgtgaacctt
    20641 cgtgctcttc gtggttaaat tttaaaattg cggcaaattt ggattagcca gtatagaaaa
    20701 agaaaatcca tatat
//
LOCUS       DAOQOS010000099        20259 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_1304_length_20259_cov_6.418630, whole genome shotgun sequence.
ACCESSION   DAOQOS010000099 DAOQOS010000000
VERSION     DAOQOS010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 20259)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20259)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20259
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1304_length_20259_cov_6.418630"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..619
                     /locus_tag="PLF66_18655"
     CDS             <1..619
                     /locus_tag="PLF66_18655"
                     /inference="COORDINATES: protein motif:HMM:NF014533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HMZ66118.1"
                     /translation="ATSLLPDLLVKSNQLGIVSGVATGIDRTAMLSSLNANIPTIGVL
                     GTGLEKEYPFQNKDLYKKIKSSDHGLLISEMRIGETIGKWSFPRRNRIITGISKLLIL
                     MEAPIKSGAMSSVAHAIEQGREVLVFDEPELLYNEGGRKLIEEGATKLTLSDLKKSPD
                     SFFHISEIIPNDFKDIPSLFRSLNQLEREGLFQNMGGGYFKKKSS"
     gene            777..1256
                     /locus_tag="PLF66_18660"
     CDS             777..1256
                     /locus_tag="PLF66_18660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015682540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HMZ66119.1"
                     /translation="MHPNEELINKFYTAFQKKDPKTMGECYADTVEFNDPVFSGLKGN
                     AARAMWQMLIERSNNLNLTFSNIKANDTEGSADWIAVYPFSKTGRTVTNHIHAEFKFQ
                     DGKIVSHNDHFNLWKWAGMALGITGYLTGFLPAVQKKIKDEANTGLTMYMKRKKITV"
     gene            1371..1583
                     /locus_tag="PLF66_18665"
     CDS             1371..1583
                     /locus_tag="PLF66_18665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008593870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HMZ66120.1"
                     /translation="MESFGKNLIFLGVILLIVGALFTLSGKFPFIGNLPGDISIKKEN
                     FSFYFPLTTSILISILLSLIMWLWNR"
     gene            1617..1979
                     /locus_tag="PLF66_18670"
     CDS             1617..1979
                     /locus_tag="PLF66_18670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HMZ66121.1"
                     /translation="MKKIKLILTFLFAVFIIFGGINHFLKPSMYLPFIPNFLPKELVN
                     YASGVLEILIGIGLLIPKYRFRSAQALLLLMIAFLPIHVWDVFRDDPAIGSHSLALVR
                     LPFQFLFIAWAWFITKRD"
     gene            2214..4229
                     /locus_tag="PLF66_18675"
     CDS             2214..4229
                     /locus_tag="PLF66_18675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011671206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HMZ66122.1"
                     /translation="MRYLLILAIAFSSLFAENPSDLANNHFGFPLNTQNVEYNPIISP
                     NGRYIVFQSNRPGGQGGMDFWLSENRNYRDRTGKANWMEPVNLNELSTMGFDGPFTIQ
                     FDEEGRPKEIYFTSIRSEATGKDGYKGLNIYYTHREASIDKWSVPIHLNDLNSDFDDK
                     MPTISPDGRYLVFSSNRPGGFGEFDLWISERVNKSEDPNPKWSRPINIGNKVNSAANE
                     IMPYFHYDNLTLYFSSDRNDEYHKFNFFGIDMDEQFEKETISDKSSPGSIPKKAIVWK
                     EVYKLPKPFNSSMDDEGISLTHDGIWVYYASNRDGGEGQFDIYRAKVPEEMRKPYLFD
                     LSGIVIDGSEETMIGIASTIKISTNKGVVGIVTSERIGGDISKNNPKNFSTKLYTNYL
                     YKLEVSAPDYYPTEFTLDLRGSVGMKKSKYVKIVLMPIKKEEPVEVPTQAKDTPREPT
                     KDVKDTPANTDKPAKEEKKSEGLTVILRDFKTKKEILNGSVQIYTEAEKNGIQLKKEK
                     EKFILPEIPANGFELHGKAPGYQDETIIIKEGDDTPANMTIEIFLRMTKDVDKIYNTI
                     LYYELNESKLTKENIKLLDKIAAFLKLNVQDSIEIGGHTDNIASKEFNVKLSDKRAEI
                     VKEYLVSKGIDEKRIKIKAYWYSQPQAGNDTEDGRAKNRRVNFKKLN"
     gene            4241..5407
                     /locus_tag="PLF66_18680"
     CDS             4241..5407
                     /locus_tag="PLF66_18680"
                     /inference="COORDINATES: protein motif:HMM:NF019245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1577 domain-containing protein"
                     /protein_id="HMZ66123.1"
                     /translation="MATDTKPKKESFRKMTTVHQTPREWESVETNIDKMLEYVSQNNL
                     LKDPYIKLYNGKPILTVRPGSSKNSFILEVLSENNLNSDREIILYKYAREKYFELVFE
                     RLSSKEKAHVCTLRQIKIARQTRRTERFITDKNPVHAGNFLVAKIDLDIKSALGFSTE
                     VIFNEVDKTLKAKYPRSKIVPLFNRYDLKEEEELVRKHKVPIFITNTSKMESYKEIET
                     FDLKTHYEEEFMLDEKVAFFRKEMIQSFLYYPIIFKSATEEVVIGYCYNESQTPITQG
                     NLEYFEIIAYTIKKRIMDSSTASVDVKQNIVNVSEHGVLIEVTSDYIFQAIKTKPSFS
                     MDIVFKMSVPMRFSLYAKHIYQIEDTYYIGAEITGSNNEEKGFDKYKDIVRAFK"
     gene            5533..6858
                     /gene="hisS"
                     /locus_tag="PLF66_18685"
     CDS             5533..6858
                     /gene="hisS"
                     /locus_tag="PLF66_18685"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015677118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HMZ66124.1"
                     /translation="MSKSEKKISTENYKGTRDFYPDDMRFRAWMFGVMRKTVLSYGYE
                     EYDAPLLESYELYKAKTGEEIVGRQLYDFKDKGERHVAIRPEMTPSLARMVAAKNRDL
                     PKPIRWYSIPNLWRYEAPGHGRLREHWQLNVDIFGINSYQAEVEIIKLACDILFAFGA
                     PKGSFKVKISHRKILSGFLKNALGLKEESEQAIAKILDKKNKISKEEFEGQVKTHLQD
                     SEKQLSLIYKYLDSNLENISEINGIESSVIQDINHLFTTLGKLGLADYIEFDPSIIRG
                     FDYYTGFIFEIFDTNPANKRSLYGGGRYDNLIGLFSNDELSGIGFGLGDVTLQNFLKS
                     HNLIPDFKRTTTLFIPLMNDSISVEVMLLANELRQNGIHCEVMLEAKQKFGKQLSIAE
                     KKGYRYVLILGEDEIQKKVVILKDLVSSEQKIINRNSLLTDLKKEINID"
     gene            6851..7375
                     /locus_tag="PLF66_18690"
     CDS             6851..7375
                     /locus_tag="PLF66_18690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020771956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HMZ66125.1"
                     /translation="MINSLIQIDLTISRFIHDNIKNKTLDRILSRVNRGETLLFILIL
                     SFFFYTEHKFYIVFLHVGIITFLTDRLVLFLKKTISRKRPLIRVMQNEDKNPDMKHSF
                     PSAHAANSMISIFILVTAYNYSPYFFLFSLFAGIGRLITLHHFVSDILGGWTIGLLIG
                     FLNLGIMALLTSFH"
     gene            7577..9397
                     /locus_tag="PLF66_18695"
     CDS             7577..9397
                     /locus_tag="PLF66_18695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004500968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HMZ66126.1"
                     /translation="MKRFSFFLTTICFTFLFSTVAYCEPTAKKPTRTTNFTYRDFESV
                     VQAVDKHYIDKNIDVDRAYTDAAVFAMLSLPHALYLYPESYFKDREQYEEKDDILPGS
                     TFKLSESDQFLIFDPNYKKIEEIRKARAKTEAAKPKASNDELKKLIEREQIRKSILAS
                     RWEQIKFGKKDFDRVITFITENISKYKTQPMKDTYESIDEDEEVKEEFSMKDVYLAAA
                     NGYLNSLDPHSNVFLREAWEKSMKQIEDSSFEGIGAILSGGGSREVIVENPLEDKPAV
                     KAGVRAGDVILAVDDKKTKGVLLDKVVKRIKGKKGTTVKLTIKRKGVAKPFDISVVRA
                     NIEIKNVSKRLIKDHEHIAYIKLSGFVKSNTESSDTEIKNAFKELEQEAAQKNIKLKA
                     LVLDLRNNAGGYLDLAIDISDMFITKGLIVSTKSPNREADESYARMKDLTDLPLAILI
                     NAKSASASEIVASAIKHHGRGLLLGERTFGKATVQKLMDLQSNSDYVLKITQSRYYSP
                     SGKTIQVVGVEPDIQVSAEEDGSFPFQYREENMWHHLPKIPSEGKHKDYFNVEKLKDW
                     VTKNGTAEKFLKEHKNDPIKPDYQLIRSIDYVEAMLNSNQ"
     gene            9394..10992
                     /gene="nadB"
                     /locus_tag="PLF66_18700"
     CDS             9394..10992
                     /gene="nadB"
                     /locus_tag="PLF66_18700"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004280089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HMZ66127.1"
                     /translation="MRTDFLIIGSGVAGLFCAHKLSAFGEVTIITKKFDYESNTNYAQ
                     GGIASVFSKTDNPEMHLKDTLDAGAGLCDIEATRILVEEGPSRVQELVDLGVPFVKDS
                     KGELDLGLEGGHRKNRIVHAFDRTGREVERTLLSTVKANPNIKILEHHTCVDLITGHH
                     IKSNQRQNEISCYGAYILDSKNSEIFPILAKQTVLATGGAGQVYLHTTNPSIATGDGV
                     ACAYRAGAIIKNMEFFQFHPTSLYHDSGESFLISEAVRGKGGILKRMNGEAFMQEYHP
                     MKDLAPRDIVARAIDNELKKSGESHVYLDVTHINKSEIIEHFPSIYEKCKSLGIDITE
                     MPIPVVPAAHYMCGGVATDILGRTNIQNLFASGEVACTGVHGGNRLASNSLLEGLVFS
                     HRIAEHIIKEKKGFTKEHDLIPEWDKEGLKNTEEWVLISHDLHEIKSIMNDYVGIVRS
                     NLRLERAQRRIELIYKEVVDYYNRTIITNALLELRNLVQVADLVIRCAMMRKESRGLH
                     YSTDYPENRNPSRQDTIIQNTEIR"
     gene            complement(11171..12262)
                     /locus_tag="PLF66_18705"
     CDS             complement(11171..12262)
                     /locus_tag="PLF66_18705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66128.1"
                     /translation="MNRIIFIKKVLVSTQTQNALFLKKNLKQIVNIFILSFCFLNLSF
                     CIRQESRSSETPEPIAEEVKEAEYQPKEDNPSYNEQISCHTQDCIRLEYNKYSELDGK
                     INDLMAENAGKILIKMLSYDKNLNHSELEPTVDYLREISKRDGRVSIEPYYIGHRDIL
                     SEIPFVKNIGLLGWDVYGRARDAVKYRFTKNYNAKVLYHPKHHSIMMIFFVHKNYGDV
                     CNTIYSNCREVEYLDDDTFDLSLSTALKEATVKGQSVKVHFRQVKANLPTAKLDIENL
                     KSMNQSARIFKWLVATKKAEKKSVKRDRFLGIEAVVTIIDYSIKLYDAIQKYKIYSPA
                     FSTRAEVIYSGEETGGEIRSVVFYPEKNN"
     gene            complement(12278..12904)
                     /locus_tag="PLF66_18710"
     CDS             complement(12278..12904)
                     /locus_tag="PLF66_18710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016758941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HMZ66129.1"
                     /translation="MKKKISKIAEDNKNNLQTDSSDHIIETVKENLKNIRHNRKLSLD
                     KLALRCGVSRAMLSQIEQGKSAPTISVLWKIAGGLNVPFSELIKDKAREGVQVLRYEN
                     TKVLFSNTKVFSTRALFPFSGHKKTEFYELVLKPGGHEVAEPHPPGTTENIVVVTGRL
                     RLRVGEDVQELGAKDAIYFNADVPHEYSNPTEEEALMYLVMTYTDETN"
     gene            13015..13695
                     /locus_tag="PLF66_18715"
     CDS             13015..13695
                     /locus_tag="PLF66_18715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66130.1"
                     /translation="MKYRAAILGASGAVGSSLLTHLLDSSNCETILSLGRRSIDLEKL
                     SPNFSKCKQEIIDFENFTIPSDLLSGIDSAYCTLGIGQPSKVSKEEFWKIDVEYVSRY
                     AKLCADAGVRYFSLLSAVDANSDSNVYYLKAKGVLQERVIHSGFKGVYIYQPSLLATD
                     QARYGFFDSLVQFTFPLIAPILPSRYHQIHVKSLGKAMCTRAEKAITSKEVGVTEFLT
                     YNDFLKHL"
     gene            complement(13710..15116)
                     /locus_tag="PLF66_18720"
     CDS             complement(13710..15116)
                     /locus_tag="PLF66_18720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66131.1"
                     /translation="MKKLSIGIGIFFFLLVAIIVTVILSIRSIVTREFIVNQLESSFN
                     VRADLRQININLFSGLSSVELEGLDIYTRDNFADNAVELEKRTPVTGSILSIQKIDLK
                     LNFLALLRRSFQVKNFVFIGPFVSIVLSENGNNLSYLFQKPKIVEGKPNPNLSEKTEE
                     SKETKEEPFTIHSIPLAAQVGKIGIENGKLEIFIQKTQQKISLEKLNLLLTDIDIDPA
                     NLEKHNSIHLKLNTNISVFNSEGRESALFKILSNGTIVPFVPKTGYINPSIDYYLTMQ
                     QGSYIDGLSILDALSGKLPILADAGIKMDSLSKKAEILRDVEIALNYSNQRITFLKEI
                     LFPTANYDLTLESKSWLHIGNNEHNFKGAVLASKQESEQAIGGVDAAIKSKLKKGDPV
                     EIRNKLLGNIVKADRMNLPFTSSGNIKSPNVQLDVSLPSILELTKGIIGDVIKDELKK
                     KLPPGATEVINNGLKKLF"
     gene            complement(15202..16002)
                     /gene="whiG"
                     /locus_tag="PLF66_18725"
     CDS             complement(15202..16002)
                     /gene="whiG"
                     /locus_tag="PLF66_18725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004458213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor WhiG"
                     /protein_id="HMZ66132.1"
                     /translation="MSRLLDKYNQYDETSLWKDYRVSKNAEIRAYLVEKYSPLVKHVA
                     GRVAIGMPQNVEFDDLVSYGVFGLLDAIEKFDPAREIKFKTYAMTRIRGSIFDELRSV
                     DWIPRSIRQKAKQLEQIIGMLENKEGQTVEDELIAKELGVSVDELATLLTKISGTSLV
                     SLNDIWFLGDENDEVSFMETLESPANMNPDVIIEKEEIKNVIVEAIKTLPEKEKKVIV
                     LYYYEDLTLKEIGEVLEVTESRISQLHTKAVMRLRSKLGKVKSVVTKK"
     gene            complement(17140..19200)
                     /locus_tag="PLF66_18730"
     CDS             complement(17140..19200)
                     /locus_tag="PLF66_18730"
                     /inference="COORDINATES: protein motif:HMM:NF022481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HMZ66133.1"
                     /translation="MKSKFISLFLFIFSFLFLWFSRPAGVGLEDSGGFFLSACNFGIS
                     HPPGYPLFNLLGGLSRLTTKSFLDCIDSILLLNISLASGIIVFCYQFLMLFIRDNLGF
                     YKKAIVAIISSLALLVSRSFSFLARSSEVYILHLFFVIFLLFILLKLKKEGNVFYKNV
                     FYFVLGLSFANHWPLILILFPFFISYIYYLEIKKNIFQDIIKSFVFFAFGFSIYIYYF
                     INPFVSEYYFYSKVETIAEAIHLFLRKDQSVMDVLPTWELMNSFYFTFHFFERISFEF
                     GVIVSLLGVIGTLLCYPIFILCISIPLFLPFLWRTEVNQFTLEMFQYWELSSYIAVII
                     GFSLFMNFILVRLKNERIKNFYAIGIFALGAIYISFSILNQAQFKKDSLASDYSRLVL
                     DSLPKNSILFVHKDTEAGIIGFENYGREYRKDIDVISQVSALFPKRVFLRKKNPNRES
                     QRLPILNFISKELDKGRRVFTTRRLEQFNELVPFPMKYSEYGIYFEINYEKIDVSLQG
                     DSIQSIKNILILENDRRKKNQGQSIFISKYNLPWKRYREFLTRDLCHTLLIFEGTIDG
                     IENLPECRFIYAQWIHVNQKQYELAEEEFRNSLSNSTYLYKSELVERYRDFLLNRISY
                     INSLKVSKEEKRKYLLDTIVSVKSIALDYKYCENKLAKILLNLANELSLKEEDLIEWN
                     KTCK"
     gene            complement(19205..19690)
                     /locus_tag="PLF66_18735"
     CDS             complement(19205..19690)
                     /locus_tag="PLF66_18735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002973119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgmX/PglI C-terminal domain-containing protein"
                     /protein_id="HMZ66134.1"
                     /translation="MDRNKIFIVASVLIVFVFGYFLFERSKKETIAPLQIETDKKGLS
                     HYHKREVNNTIKKHMGSIQSCYNQMLEKNPKVTEGKIQFDWQIKPDGDVVKAEKIQSD
                     FDSTDLEDCIKKEISSWSFPPPPEQNRNTYSEFTFHFVKKENLEKQKDNAPKMINTNS
                     K"
     gene            complement(19700..>20259)
                     /locus_tag="PLF66_18740"
     CDS             complement(19700..>20259)
                     /locus_tag="PLF66_18740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66135.1"
                     /translation="ETKSTNENLDIANDSKKTEKEKIEFISKWGYVWRSALIPGWGHI
                     KAEYYKTGFFYGTLFYGSLISAGYFTNQLNQAIKQRDEKIDTNTFLTLGAGGPGSALL
                     LFAGGSSSTTSDDQKILAYRSLLYGSAGAAVGVYIIQLINVYVIGVKIEKSRVSGINF
                     YSVPEFSSLTKEVGMQNGISYTISF"
BASE COUNT         7107 a         3477 c         3303 g         6372 t
ORIGIN      
        1 agctacaagt cttttaccgg acttattagt aaaatcaaat caattaggaa tcgtttcagg
       61 cgttgctaca ggcattgatc gcacagccat gctttcttct ctaaatgcaa atattccaac
      121 cataggtgta ttgggaactg gacttgagaa agaataccca tttcaaaaca aagatttgta
      181 taaaaaaata aaatcttcag accatggttt acttatctct gaaatgagaa tcggtgaaac
      241 aatcggcaaa tggtcttttc ctcgacgaaa tagaattatc actggaatct cgaagctatt
      301 aatcttaatg gaagcaccta taaagagtgg ggctatgtca tctgtcgctc atgccataga
      361 acaaggcaga gaagtccttg tatttgatga acccgaattg ctatacaatg aaggaggaag
      421 aaagctgatt gaagaagggg caacaaaact cacactttct gatttaaaaa aatctcccga
      481 ctccttcttt catatttcag aaatcattcc aaatgacttc aaggatatac ctagtttatt
      541 ccgatccctg aatcagctag aaagagaggg gctttttcaa aatatgggag gtggatattt
      601 taaaaagaaa tcatcctaat ctagaatctc tatttaaaca cagattttaa acagaataag
      661 ctatttttat aaacccattt tatctttttc taattaattg gaataatcat ttgacaattt
      721 ccgaataaaa atccgaaatt tgattcagca aatataaaaa tggaaaagga attataatgc
      781 atccaaacga agagttaatt aataagtttt ataccgcttt tcaaaagaaa gatccaaaga
      841 caatgggtga atgctatgct gatacagttg aattcaatga tccagtcttt agtgggctca
      901 aaggaaatgc agctagagct atgtggcaaa tgctgataga aagaagtaat aatctaaatc
      961 taaccttcag caatatcaaa gcaaatgata cagaaggaag tgcagattgg atagcggtat
     1021 atcctttcag caaaactggt agaactgtaa caaatcacat tcatgccgaa ttcaaatttc
     1081 aagatggaaa aattgtttct cataacgatc atttcaatct ttggaaatgg gcgggaatgg
     1141 ctcttggcat aactggatac ttaaccggct ttttacctgc agttcaaaag aaaataaaag
     1201 atgaggctaa tactggttta accatgtata tgaagagaaa aaaaattact gtctaacttt
     1261 cctaattaaa atcgtgtaaa tgcttacttt ttcatcaaac aaataaatat tctttccttg
     1321 tgattttgta gctaagcttt tacacaaacc aaattattga gaaataccct atggaatctt
     1381 ttggaaaaaa tctaatcttt cttggagtaa ttttactcat tgtaggtgct ctgtttactc
     1441 tcagcggtaa attcccgttt atcggtaatc tacccggcga tatcagtatc aaaaaagaaa
     1501 attttagctt ttatttccct ctcacaacct ccattttaat cagcattcta ctttccctga
     1561 tcatgtggtt gtggaaccgt tgaaaaaaag ctactaaaaa caaaggagtc aaaacaatga
     1621 aaaaaataaa actaatctta accttcctat ttgcagtatt tattattttt ggcggtataa
     1681 atcatttttt aaaaccaagt atgtatcttc cctttatccc aaattttcta ccaaaggaat
     1741 tagtaaatta tgcatctgga gttttagaaa tactcattgg aatcggactc ctcattccaa
     1801 agtatagatt tagaagtgct caagcgcttc ttttactcat gatcgcattt cttcccattc
     1861 atgtttggga tgtttttcga gatgatcctg caattggctc acacagtcta gcactcgttc
     1921 ggctaccatt tcagtttctt tttattgctt gggcttggtt tatcaccaaa agagattaag
     1981 cttgacaatt agatgtcaag atcccccctt tttctgctcc aagatttaag aaatttaaat
     2041 tgaatagaga aaaaccaagg attaaaaagg atcaaatcta aattttcagt gaagtgtgaa
     2101 ttgtagatcg atccaccttt ggaaacgatt agaatcgcaa ccaaatattg gattcataaa
     2161 acgatttaca aaaataatag atattctagt ttttttattt cgatattaaa actatgagat
     2221 atttattaat cctagcaatt gctttctctt ccctgtttgc agaaaatccg agcgatttag
     2281 caaacaatca ctttgggttc ccactcaata ctcaaaatgt agaatacaat ccgattatca
     2341 gtcccaatgg tcgctacatc gtttttcagt ccaaccgtcc aggaggacag ggtggaatgg
     2401 atttctggct gtctgaaaat agaaattaca gagacagaac tggaaaagcc aattggatgg
     2461 agcctgtcaa tctaaacgaa ttgagcacaa tgggtttcga tggacccttt acaattcaat
     2521 ttgatgaaga aggtagacca aaagaaattt attttacatc tatcagaagt gaagcgactg
     2581 gcaaagatgg ctacaaaggt ctaaatatat attacactca tagagaagct tcgattgata
     2641 aatggtcagt gccaattcac ttgaatgatc tcaactccga ctttgatgat aagatgccaa
     2701 cgatatcgcc tgacggtaga tatttagtat tttcatccaa tcgtcctggt ggctttggag
     2761 aatttgatct ctggatttct gaacgtgtga ataaatcaga ggatccaaac cctaaatggt
     2821 ctcgtccgat caacattgga aataaagtca attctgcggc taatgagatt atgccctact
     2881 ttcactacga taatctaact ctctatttta gttctgatag aaatgatgaa taccacaagt
     2941 tcaatttctt tggtattgat atggatgaac aattcgaaaa agaaacgata agtgataaat
     3001 ctagtcctgg aagcattcct aaaaaagcaa ttgtatggaa agaagtttat aaactaccaa
     3061 agccatttaa tagttctatg gatgatgaag gaatttcact cactcatgat ggaatctggg
     3121 tttattatgc atcgaatcga gacggtggag aggggcaatt cgatatttac cgcgcaaaag
     3181 ttccagagga aatgagaaaa ccctatttat ttgatttaag cggaattgta attgatggat
     3241 cagaagagac aatgatcggg attgcatcga ctataaaaat ttcgacaaac aagggagttg
     3301 taggaattgt aacatctgaa cgtattggtg gtgatatctc aaaaaataat ccgaaaaatt
     3361 tttctacaaa actctatacc aattatttat acaaattgga agtatctgct cccgattatt
     3421 atccgacaga atttacttta gatctacgcg gttctgttgg gatgaaaaaa tctaaatatg
     3481 taaaaattgt cttaatgcca attaaaaaag aagagccagt agaagttccg acacaagcaa
     3541 aagacacacc aagagaacca acgaaggatg taaaagacac tccagcaaat acagataaac
     3601 ctgcaaaaga agaaaaaaaa tcagaaggac tcactgtcat actgagagat ttcaaaacta
     3661 aaaaagaaat tctaaatgga tccgttcaaa tttatacaga agcagaaaaa aatggaatcc
     3721 aactcaaaaa ggaaaaggaa aaatttattc ttcccgaaat tcctgcaaat ggcttcgaat
     3781 tgcacggcaa agccccaggc taccaagacg aaaccatcat tatcaaagaa ggtgatgaca
     3841 cacctgccaa tatgacgatt gaaattttcc ttcgaatgac taaggatgta gataaaattt
     3901 ataatactat tctctattat gaattgaatg aatctaaatt gacaaaggaa aatataaaat
     3961 tacttgataa gatagcagct ttccttaaac tgaatgtcca agattcaatt gaaattggtg
     4021 ggcatacaga caacattgcc agcaaagagt tcaatgttaa gctaagcgac aaaagagcag
     4081 aaattgtaaa agaataccta gtctcaaagg gaatcgatga gaagcgaatc aaaataaaag
     4141 catattggta ttcacaaccc caagctggaa atgatacgga agatggcaga gccaaaaacc
     4201 gtagagtaaa tttcaaaaag ttaaactaga ggaaataaaa ttggctacag atacaaaacc
     4261 caaaaaggaa tcattccgta aaatgactac cgtccatcag actcctaggg aatgggagtc
     4321 tgttgaaact aacatcgata aaatgttaga gtatgtatct caaaacaatt tattaaaaga
     4381 tccttatatc aaactataca acggcaaacc gatattaacc gttcgaccag gaagttctaa
     4441 aaattctttt attttagaag tattgagtga aaataatctc aactcagata gagagattat
     4501 actctataaa tacgctcgag aaaaatattt tgagttagtt tttgaaagac ttagctctaa
     4561 agaaaaagcg cacgtttgca cattgcgcca aattaaaatt gctcggcaaa ctcgaagaac
     4621 ggaacggttt ataacagaca aaaatcctgt tcatgccgga aatttcttag tagcgaaaat
     4681 tgatctagat attaaatccg cacttggatt ttcaacggaa gtaatcttca acgaagttga
     4741 caaaacacta aaagcaaaat acccgcgctc caaaatagtg cccctcttca atcgttatga
     4801 tctaaaggag gaagaggaat tagttcgtaa gcataaggtg ccaattttta ttacgaacac
     4861 atctaaaatg gaatcctata aagagatcga gactttcgat ttaaaaactc attatgaaga
     4921 agaatttatg ttagatgaga aagtagcttt ttttagaaaa gagatgattc aatcctttct
     4981 ctattatccg atcattttta aatcagctac agaagaagta gtgatcggat attgctacaa
     5041 tgaaagtcaa actccaataa cccaagggaa tttagaatac tttgaaatca tagcctacac
     5101 gatcaagaag agaatcatgg attctagcac tgcaagtgtg gacgtaaaac aaaacattgt
     5161 aaacgtttct gaacatggag ttttaataga ggttacatct gattatatat ttcaagctat
     5221 taaaacaaaa cctagctttt ctatggatat agtttttaag atgagtgtgc caatgcgatt
     5281 ttctctctat gcaaagcata tttatcaaat cgaggatacc tactacattg gagctgagat
     5341 cacaggctca aacaatgagg aaaaaggatt cgacaaatat aaggatattg taagagcatt
     5401 caaatagtag aatgaagtga gtaagtaatt cttacaaagc aagatttttc tttgatttta
     5461 tatcaaaata aatcatatgc aaatataggt ctaatcctaa aaaattttaa attaatctta
     5521 attaggtttc acttgagtaa atcagaaaaa aaaatttcaa cagaaaatta taaaggaaca
     5581 cgcgattttt atccagacga tatgcgtttt agagcatgga tgtttggtgt tatgcgcaaa
     5641 acagttctat cttacggcta tgaagaatat gatgcccctc ttttagaatc atatgaatta
     5701 tacaaagcaa aaacgggcga agagattgtt ggtcgacagc tctatgactt caaagacaaa
     5761 ggggaaagac atgttgccat acgccccgaa atgactccat cactcgctcg tatggttgca
     5821 gccaaaaata gagatttacc aaaaccaatt cgttggtatt ccattccaaa cctttggcgc
     5881 tatgaagctc ccggtcatgg aagacttaga gaacattggc aactaaacgt tgatatattt
     5941 ggtattaatt cctatcaagc cgaagttgaa atcattaaac tagcctgtga tattctattt
     6001 gcctttggtg ctcctaaggg aagtttcaaa gttaaaattt ctcaccgaaa aattctttct
     6061 ggttttttaa aaaatgcgtt aggtctcaaa gaggaatcag aacaggcaat cgctaaaatc
     6121 ttagataaaa aaaataaaat atcaaaagaa gaatttgaag ggcaagttaa gactcactta
     6181 caagacagtg aaaaacaatt atctcttatt tacaaatatc tagattctaa tctggaaaat
     6241 atatccgaaa taaatggaat tgagtctagt gttattcaag acatcaatca tctttttaca
     6301 actcttggaa aattaggttt agcagattat attgagtttg atccttctat cattcgagga
     6361 ttcgattatt atacaggttt tatttttgaa atctttgata cgaatccagc taataaaaga
     6421 tctctctatg gtggtggccg ctatgataat ctaattggac ttttttctaa tgatgagtta
     6481 tctggaattg gatttggttt aggagacgtt actctacaaa attttctaaa gtcacacaac
     6541 ttaataccag attttaagag aactactact ttatttattc cactcatgaa tgattctatc
     6601 tctgtggaag taatgctttt agctaatgag ttgcgacaaa atggaattca ttgcgaagta
     6661 atgctcgaag caaaacaaaa attcggtaaa caattatcaa tcgcagaaaa aaaaggatat
     6721 cgttatgtcc taattttagg cgaagatgaa atacagaaaa aagtagtcat actaaaagat
     6781 ttagttagta gcgagcaaaa aataataaat agaaatagtc ttctcactga cttaaaaaaa
     6841 gaaattaaca ttgattaact cgcttataca aattgatctc acaatctcta gatttattca
     6901 tgataatatc aagaataaaa ctctagaccg aattctctcg cgagtcaata gaggagagac
     6961 gttactcttt atattaattc tctctttttt cttttatacg gagcataaat tctatattgt
     7021 ttttttgcat gtaggtatca taacgttttt aacagataga cttgtattat ttcttaagaa
     7081 aacaatttca cgtaagcgtc ctctgattcg agtcatgcaa aatgaagata aaaatccaga
     7141 catgaagcat tctttccctt ccgctcatgc agcaaattca atgatttcaa ttttcatctt
     7201 agtgactgca tataattact caccctattt ctttctattc tccctttttg ctggaatcgg
     7261 cagattgatt acattgcatc attttgtgag tgatatttta ggtggctgga caatcggttt
     7321 gctcattggt tttttaaatc ttggaatcat ggctttacta acaagttttc attaatgcaa
     7381 ttccgactgt tatttcagta aaacacagcc tttttaagta aggtttgatt ctactctttc
     7441 ttgctctcag attaaatttt ttgtagttaa tccgaattag actctcatat agaacaattc
     7501 ttccaattac aaatttattg acactcttag gtcaaatttt atattgagat aactcaaatc
     7561 tagagggtat acagttttga aaagattctc tttttttcta accacaattt gtttcacttt
     7621 ccttttttcc acagttgcct actgtgaacc gactgccaaa aagccaactc gcactacaaa
     7681 ttttacatac agagactttg agagtgttgt gcaagctgtt gataaacatt acattgataa
     7741 aaatattgat gtcgatagag cctatacgga tgccgcagtt tttgcaatgt tatcgttgcc
     7801 tcacgctctc tatctctatc ccgaaagtta ttttaaagat agagaacaat atgaagaaaa
     7861 agacgatatt ctccctggct cgacattcaa gctatcagag tcagatcaat ttctgatttt
     7921 cgatccgaat tataagaaaa ttgaagagat cagaaaagcg cgagcaaaaa cagaagcagc
     7981 aaaaccaaaa gcatcaaatg acgaattaaa aaaactcatt gagcgtgaac aaatccgaaa
     8041 atctattttg gcttctagat gggaacagat taaatttggt aagaaggatt ttgatcgagt
     8101 cataacattt ataacagaaa atataagtaa atataaaact caaccaatga aagatactta
     8161 cgaatcgatt gatgaggatg aagaagttaa agaagaattt agtatgaaag atgtttatct
     8221 tgcagcagct aacggctatt taaattcatt agatcctcac tccaacgttt tcctaagaga
     8281 agcatgggaa aaatcgatga agcaaattga ggattctagc ttcgaaggaa ttggggcaat
     8341 tttaagtggt ggtggtagca gagaagttat tgttgaaaac ccattagaag acaaaccagc
     8401 agtgaaagcc ggtgttcgag caggcgatgt aattcttgct gttgatgata aaaagacaaa
     8461 aggtgtttta ttagacaaag ttgttaaaag aatcaaaggc aaaaaaggaa ccactgttaa
     8521 gttaaccatc aaaagaaagg gtgttgcaaa accttttgat atatctgttg ttcgagctaa
     8581 tattgaaatt aaaaatgtat ccaaacgatt gattaaagat catgagcata tagcctatat
     8641 aaaactttct ggctttgtaa aatcaaatac agaatcctca gatactgaaa ttaaaaatgc
     8701 ttttaaagag ttagaacaag aagctgcaca gaaaaacatt aaactcaaag cgttagttct
     8761 agacttaaga aataatgcag gtgggtattt agatttagca attgatatca gtgacatgtt
     8821 cataacaaag ggcttaatcg ttagcacaaa aagcccaaac agagaagcag atgagtctta
     8881 tgcaagaatg aaggatttaa ctgatttacc attagctatc ttaatcaacg ctaagtctgc
     8941 gtctgcaagt gaaattgtag caagtgcaat taagcatcat ggaagaggac tccttcttgg
     9001 agaaagaact tttggtaaag caacagttca aaaattgatg gatctacaaa gtaacagtga
     9061 ttatgttttg aaaattactc agtccagata ttactctcct tcaggaaaaa caattcaagt
     9121 tgtaggggtt gagcctgata tccaagtatc cgctgaagaa gatggtagct tcccattcca
     9181 atatagagaa gaaaatatgt ggcaccattt acccaagatt ccttcagagg gtaaacacaa
     9241 agactatttc aatgttgaaa agttaaaaga ttgggttaca aaaaatggaa cggctgagaa
     9301 gtttttaaaa gaacataaaa atgatccaat taaaccagat tatcaattga tccgttccat
     9361 cgattacgtg gaagcaatgc tcaattcaaa ccaatgagaa ctgatttttt aatcattggg
     9421 agtggcgttg ctggattatt ttgtgcacac aaactatccg cttttggtga agttacaatc
     9481 attacaaaga aatttgatta tgaatcaaat acaaattatg ctcaaggcgg aatagcatct
     9541 gtattctcaa agactgataa ccctgaaatg catcttaaag atacgttaga tgcaggagca
     9601 ggtctatgtg atatagaagc aactcgaatt ttagtagaag aaggtccgtc tagagttcaa
     9661 gaacttgtag atttaggagt cccttttgtt aaagatagca aaggagaact agatctaggc
     9721 ttagagggtg ggcacagaaa aaatagaatc gtccatgcat ttgaccgcac aggaagagaa
     9781 gtagaaagaa ccctactttc tacagtaaaa gcaaatccaa atattaagat tctagaacat
     9841 catacctgtg tagatttaat tacaggacat catataaaaa gcaatcaaag gcaaaatgaa
     9901 attagctgct atggtgcata catattagat tccaaaaatt ccgaaatttt cccaatcctt
     9961 gccaagcaaa cagttcttgc aacgggcggt gcaggacaag tttatcttca tacaacaaat
    10021 ccatccatag caacaggaga tggtgtagct tgtgcatata gagcaggcgc aattattaaa
    10081 aatatggaat tttttcaatt ccatccaact tcactctatc atgattccgg agaatccttt
    10141 ttaatatcag aagcagtaag aggaaaaggt ggaattttaa aaagaatgaa tggcgaagct
    10201 tttatgcagg aatatcatcc tatgaaagac ctcgcgccta gagatatagt agctcgtgcg
    10261 atagacaacg agttaaagaa aagtggggaa agccatgtat atctagacgt cacgcacata
    10321 aataaatcag aaatcatcga gcattttcca tctatatacg aaaaatgtaa atctcttgga
    10381 attgatatta cagaaatgcc aattccagtt gttcccgcag cacattatat gtgtggagga
    10441 gttgccactg atatattagg cagaactaat atacaaaatt tatttgcaag tggtgaggta
    10501 gcctgcactg gagtgcatgg aggaaataga ttagcatcaa atagtttatt agaaggacta
    10561 gtattttcac atcgaatcgc agaacatatt atcaaagaga aaaaaggttt tactaaagag
    10621 catgacctca ttcctgaatg ggataaagaa gggctaaaaa atacagaaga atgggtcctc
    10681 atttcacatg acctacatga aattaaaagt attatgaatg attatgtggg tatagtcaga
    10741 agcaatttaa gattagaacg cgcacaaaga aggatagaac taatctacaa ggaagttgta
    10801 gactattaca atcgaacgat tataactaac gcacttttag aacttagaaa tttggtgcaa
    10861 gtagctgatt tagtcattcg atgtgcaatg atgcgtaaag aaagtagagg tcttcattat
    10921 tccacagatt atcctgaaaa tagaaatcca tccagacaag atacaatcat tcaaaacacc
    10981 gaaataagat aattggatct tggactagaa ttagcgaata gaatcggaat tatcgtctgc
    11041 tcgatataac aaaaaattga aaagattacc accgataaat gatgcgataa tttagaatta
    11101 tccatcattt cttcctcgtt caattatcgg tgtgcgtcgg tggtaaaaaa attcttaggt
    11161 cgaattcagg ctaattgttc ttttctggat aaaaaactac tgatcttatt tctccaccag
    11221 tttcctctcc actataaatc acttctgctc tagtagaaaa agcgggagaa tatattttat
    11281 atttttgaat tgcgtcatat aattttatag aataatcaat aatcgtcaca actgcttcta
    11341 ttccaagaaa tctatctcgc ttaacgcttt tcttctcggc ctttttagta gcaacaagcc
    11401 atttgaaaat tctagcagat tgattcatac tttttaaatt ttcaatatct aattttgcag
    11461 taggcaaatt tgctttgact tgtctaaaat gaactttaac agattgtcct tttacggtag
    11521 cctctttaag agcagtcgat aaacttaaat caaatgtatc atcgtctaga tattcaactt
    11581 ctctacaatt tgaatagatt gtattgcata catcgccata atttttatgc acaaaaaaaa
    11641 tcatcatgat tgaatgatgt tttggatggt ataaaacctt agcattataa ttttttgtaa
    11701 atctatattt aactgcatct ctagctctac cataaacatc ccaaccgagc agaccaatat
    11761 tttttacaaa aggaatttct gataaaatat ctcgatgccc gatataataa ggttctatag
    11821 aaacacgacc atctcttttt gatatttcac gaagataatc cactgtaggt tctaactcag
    11881 aatgattcaa attcttgtca taagataaca tttttattaa tatttttccg gcattttctg
    11941 ccattaaatc attaatcttt ccatccaact cggagtattt attatactcg agtcggatgc
    12001 aatcttgagt atgacaagat atttgctcat tgtaagaagg attatcctct ttcggttgat
    12061 attctgcctc ttttacttct tcggctattg gttctggcgt ttctgaactt ctagattcct
    12121 gacgtatgca aaaagaaaga ttcaaaaagc aaaaggagag aataaaaata ttcacaattt
    12181 gttttaagtt ttttttcaga aataaggcgt tttgcgtttg ggtcgatact aaaacctttt
    12241 ttataaaaat gattcgattc atttaaagaa tccttattta atttgtctca tctgtataag
    12301 tcataactaa atacataaga gcctcttctt ctgtagggtt tgaatattca tgaggaacat
    12361 cagcattaaa ataaattgca tctttggctc caagctcttg cacatcttct ccaactctga
    12421 ggcggagacg accggtaact acgacaatgt tctcagtcgt tccaggtgga tggggttctg
    12481 ctacttcgtg tccaccgggt tttaaaacca actcataaaa ttctgttttt ttgtgaccac
    12541 taaatggaaa aagtgcacgt gtagaaaaaa cttttgtatt tgaaaataaa acttttgtat
    12601 tttcatatcg taggacttgc acaccctctc tagctttatc tttaatcagt tcactaaacg
    12661 gaacgttaag tccaccagct atcttccaaa gcacagaaat tgttggagca cttttccctt
    12721 gctcaatttg ggacaacatt gccctactta ctccacaacg taaagctaac ttatcaagag
    12781 ataactttcg attatgacga atattcttta aattttcctt aacagtctca atgatatggt
    12841 ccgaggaatc tgtttgcaga ttgtttttat tgtcttctgc tattttggaa attttctttt
    12901 tcatagaaga atattccaat ataacagaca tgtgtcaatg aaaaaagtcc agaaacacct
    12961 tttccttcct aattttagct ttacataaca ggaatgaata tccaacaatc cagtatgaag
    13021 tatagagctg ccattcttgg tgcatctggc gcagttggtt caagtctttt aactcattta
    13081 ttagattctt cgaactgcga aactattcta tctttgggta gaagaagtat cgatctagaa
    13141 aaactctctc ccaatttctc taaatgcaaa caagaaataa tagattttga aaattttaca
    13201 atcccatcag acttgttaag tggaattgat tctgcttatt gcactctagg cataggacaa
    13261 ccgagtaaag tttccaaaga agaattttgg aaaattgatg tagaatatgt cagtcgttat
    13321 gcgaaattat gtgcagatgc aggagtcaga tatttttctc tactaagtgc ggtagatgca
    13381 aattctgact caaatgtata ctacttaaaa gccaaaggtg ttttacaaga aagagtaatt
    13441 cactctggat tcaaaggtgt ttatatctat caaccatctc ttcttgcaac cgatcaagct
    13501 cgctatggat tctttgatag tttagttcaa ttcacattcc cattgattgc acctattctc
    13561 ccaagccgat accatcagat tcatgttaaa tctctaggga aagcgatgtg cactcgtgca
    13621 gaaaaagcca ttacaagcaa agaggtggga gtcactgaat ttttaacata caatgatttt
    13681 ttaaaacatc tataaaccaa tggattgaat taaaataatt ttttaagtcc attgttaatc
    13741 acctccgttg ccccaggagg tagttttttc tttagctcat ctttaattac atcaccaatg
    13801 attccttttg ttaattctag aatagatggg agactaacgt ctaattgaac attaggcgat
    13861 tttatattcc cactcgatgt aaaaggaaga ttcattcgat ctgctttcac aatgtttcca
    13921 agaagcttgt ttcgaatctc aactggatcg ccctttttta atttagattt tattgctgca
    13981 tccactccgc caatggcttg ctcggattct tgtttggatg ccaaaacagc acctttaaaa
    14041 ttatgttcat tattaccaat atgcaaccaa cttttacttt ctaaggttaa atcataatta
    14101 gccgttggaa acaaaatttc ctttaaaaat gtgattcttt gatttgaata atttaaagca
    14161 atttccacat cacgtaaaat ctctgctttt ttagataagc tatccatctt tattccagca
    14221 tctgcaagaa taggtaactt accggaaaga gcatctagaa ttgaaagacc atctatataa
    14281 cttccttgct gcattgttaa ataataatca atagatggat ttatatagcc agttttagga
    14341 acgaaaggaa cgatagttcc attcgataaa attttaaaaa gggcagattc tctaccttcc
    14401 gaattgaaaa cagaaatgtt tgtattcaat tttaaatgaa tagaattgtg tttttctaaa
    14461 ttagcgggat caatatcaat atctgtcaaa agtaaattta atttttctaa agaaatcttt
    14521 tgttgggtct tttgaatgaa tatttctaac tttccgtttt caattccaat cttaccaact
    14581 tgggctgcca aaggaataga atgaattgta aaaggctctt cctttgtttc ttttgattct
    14641 tctgtttttt cagataaatt tggatttggc tttccttcta caatctttgg tttctgaaat
    14701 aaataactta gattatttcc attctcactt aaaactatcg agacaaaagg tccaataaaa
    14761 acaaagtttt ttacttgaaa gcttcgacga agcaaagcaa gaaaattcaa ctttaaatct
    14821 attttctgaa tagaaagaat agaacctgtt accggtgtcc ttttttctaa ttcaactgca
    14881 ttatccgcaa agttgtctct tgtataaata tctaatccct ctaattcaac agaagaaagt
    14941 cctgaaaaga gattaatatt aatttgcctc aagtccgctc gaacattaaa agaactttct
    15001 agctgattta caataaattc tctagtgaca atagatctta tcgatagaat cactgtgacg
    15061 atgatagcaa caagcaaaaa gaaaaaaatg ccaataccga tcgatagctt tttcaaggac
    15121 aacctccgac tacttaaaat ttaagtaaaa atatttagtt tttataattt aattcaggat
    15181 aactttgaaa acgaaaaaaa attacttctt cgttacaacc gatttaactt tgcctagctt
    15241 actacgtagg cgcataacgg cttttgtgtg tagttgtgaa attcgagact cagttacttc
    15301 aagaacttct ccaatttctt ttagagttaa gtcttcataa tagtaaagaa cgattacttt
    15361 tttttctttt tcagggagag tcttgattgc ttctacaatt acatttttaa tttcttcttt
    15421 ttcgatgatc acatctggat tcatattcgc aggtgattcc aatgtttcca taaaagagac
    15481 ttcatcgttc tcatccccta aaaaccaaat gtcatttaaa gatacgagtg acgttccact
    15541 gatttttgtt agaagagttg ccaattcatc aacagaaaca ccgagctctt ttgcaattaa
    15601 ttcatcttct actgtctgcc cttctttatt ttcgagcatt ccaatgattt gttctagttg
    15661 ttttgccttt tggcgaatcg atctaggaat ccagtcaact gaacgaagct catcaaaaat
    15721 acttccacga atacgagtca ttgcatatgt tttgaatttt atttctctag caggatcgaa
    15781 tttttcaatc gcatctaata aaccaaaaac gccgtagctc actaaatcat caaactcaac
    15841 attttgaggc atgccaatgg ctactctacc tgcaacgtgt tttacaagtg gagaatattt
    15901 ttcaacgagg tatgctctta tttctgcatt cttagaaact cgataatctt tccagagact
    15961 tgtttcgtcg tattgattgt atttgtcaag aagcctagac ataatccgat ttttattatt
    16021 ttctttatcg tttccaaatt acaatcattt tatttgattt tcttagatta tttgctgttt
    16081 ttcattctaa tttagcttgt ggcggatttt atttcatacc acatccgagg atagatggat
    16141 taaaaccatt ctcaaccctt tatctgcatg agcggtgtag aaaaaaccaa tgcccatttg
    16201 aacgaatacg gactttagag tggttgttat atttaaaatc gattctgaat atagcaacca
    16261 ctctaccaag actatacgaa ccactaaaac aaacgcgaag agaaagaatg actatccttc
    16321 ccaatcaaag tgttcagtct acgcaaaggc tctttcaatg ctcatatcgt cactcagaag
    16381 gagacgaaaa cagattgccg ctgataggtt gataggtctt agttaggttt ttctgtttta
    16441 tattcctcat tgactctatc aggccttagc ttctaaagat ttacatactc atacatgtat
    16501 tgagtcgaag tcgttgaatc gagttataaa atcagacttt tcttagatac taaagaaata
    16561 ttttgattac aactaagctc ttatagaaga gcaaaaaaag ctcctcgttc aaaacatttt
    16621 tatagatgcg aagttttgtg taatgtgtcg gaggtcagtg ctaacgaatg aaatgctttt
    16681 ataccaattc ccaaaagtaa attctgactg gaaaccagaa aaccgtcttt tgggaattgg
    16741 gaatacactt ttatgaaaaa tagcagaact tatttatgag aagtggtata taaattaaaa
    16801 cccacatccg ggtccggctt gatttgctaa catacaagaa tagaggcagt ccctatataa
    16861 atcctggcaa tatttagaag ccttaacccg aaaatcacca ttattgcagg agccatacca
    16921 catagccatt acgccataac agactatttt aaatctctca ttttcttttc tttctttttt
    16981 ctcactcctt ggctcaagtg gttcatcctt cccacaactc aaatttaaca gcaaggacaa
    17041 aagaattata atatatatga aatttcttag atgcatacta gtatactctc aataaaaatt
    17101 aacttctttg tggcaaatca aatacaaagc aaacaaaaat cacttacaag ttttattcca
    17161 ctcaattaga tcttcctctt tgagagataa ttcatttgct aaatttaaaa gaatttttgc
    17221 taatttgttt tcacagtatt tataatcgag agcaatactt ttaacactta ctatcgtatc
    17281 gagtaagtat tttctttttt cctctttact aaccttcaaa gaatttatat aggaaattct
    17341 atttaataga aaatctctgt atctctcgac taattctgat ttatacaaat acgttgaatt
    17401 tgaaagacta tttctaaatt cttcctccgc aagttcatat tgtttctgat ttacatgaat
    17461 ccactgcgca taaataaatc tacactcagg aagattttct atgccgtcta tcgttccttc
    17521 aaaaataagt aaggtatgac ataagtctct ggttaaaaat tctcgatacc gcttccaagg
    17581 caaattatac ttgcttataa aaatactttg cccttgattt ttctttcttc tatcattttc
    17641 taaaatcaga atatttttta tactttgtat agaatctccc tgaagagaaa catctatttt
    17701 ttcataattt atctcaaagt aaattccata ctcagaatat ttcataggaa atggcacaag
    17761 ctcattgaat tgttctagtc ttcttgtcgt aaaaaccctt cgacctttat ctagttcctt
    17821 agaaataaaa tttaaaatgg gtaacctttg tgattctcta tttggattct tttttctaag
    17881 aaaaactctt ttaggaaaaa gcgctgatac ctgcgagata acatctatat cttttctata
    17941 ttccctacca taattttcaa aaccaataat tcctgcttct gtgtctttat gcacaaatag
    18001 aattgaattt ttaggtaagg aatctaaaac caatctagaa taatcagaag ccaaagaatc
    18061 cttcttaaac tgagcttgat ttaaaattga aaatgaaata taaatagcgc ctaacgcaaa
    18121 gattccaatc gcgtaaaaat tttttatcct ttcatttttt aatctgacca aaatgaaatt
    18181 catgaacaaa ctaaatccta tgataactgc tatataagaa gacaactccc agtattgaaa
    18241 catttccaag gtaaactgat tcacttccgt tctccatagg aatgggagaa acaaaggaat
    18301 cgaaatacac aatataaaaa tgggataaca aagtagagtt ccaatcaccc caagtaggga
    18361 aacaatgact ccaaattcaa aactgattct ctcaaaaaaa tgaaatgtaa aataaaagga
    18421 attcatcaac tcccaagtag gcaaaacatc catcacagat tgatcttttc tcagaaataa
    18481 atgaattgcc tctgctattg tttcgacctt cgaatagaaa taatattctg aaacaaatgg
    18541 attgataaaa taataaatat aaattgaaaa accaaatgca aaaaaaacaa aagacttaat
    18601 tatatcttga aagatatttt tttttatttc taaataataa atgtaagaaa tgaaaaaagg
    18661 aaataaaatt aaaatcaaag gccaatgatt ggcaaaagaa agcccaagaa caaaataaaa
    18721 tacattttta taaaatacat tcccttcttt tttcagtttc aataaaataa ataataaaaa
    18781 tattacaaaa aacaaatgaa gtatatacac ttctgaagaa cgagctaaaa aggaaaagga
    18841 acgcgatact aataaagcca aactagaaat tatagctact atcgcttttt tataaaagcc
    18901 taggttatca cggatgaata gcatcaaaaa ttgataacaa aatacaatga ttcctgaagc
    18961 taaagaaata ttcaataaca gaatcgaatc gatacaatct aaaaatgatt tagtggttaa
    19021 tcgtgataat ccaccgagta aattaaaaag aggatagccg ggaggatgcg aaatcccaaa
    19081 attacaagcg gaaagaaaaa atcctcctga atcttctaac ccaactcctg cgggtctaga
    19141 aaaccataaa aataaaaaag aaaaaataaa aaggaataac gaaatgaatt ttgatttcaa
    19201 gcttttattt cgaatttgta ttgatcattt tgggagcatt gtctttttgt ttttcgagat
    19261 tttctttttt tacaaaatga aaagtaaatt ccgaatacgt atttcgattt tgctcgggag
    19321 gtggcggaaa actccaagaa gaaatttctt tttttataca atcttccaaa tcagttgaat
    19381 caaaatcact ttggattttc tcagctttaa caacatctcc atccggttta atttgccaat
    19441 caaattgaat tttgccttct gttacttttg gatttttctc cagcatttga ttgtagcagg
    19501 attgaatgga acccatgtgc tttttaatcg tattattcac ttctctttta tggtaatggc
    19561 ttaaaccttt tttatcagtt tcaatttgta aaggagctat cgtttctttt ttggaacgct
    19621 caaatagaaa ataaccaaat acaaaaacaa ttaaaacaga agctacgata aaaattttgt
    19681 tcctatccat aaaatttcct taaaatgata tcgtataaga aattccattt tgcattccaa
    19741 cctcttttgt taaagaggaa aattcaggaa cagagtaaaa attaattcct gaaactcttg
    19801 atttttcaat ttttactcct atcacatata catttatcaa ttgaatgata tacacaccaa
    19861 ctgcagcgcc ggctgagcca tacaaaagag aacgataggc taggattttt tggtcatcgc
    19921 tagtagtaga agaacttcca ccggcaaata gcaataaggc ggaacccggt ccccctgctc
    19981 ccaaagttaa aaaagtattc gtatctattt tttcatctct ttgtttgatc gcttggttca
    20041 actgatttgt gaaataccca gcagaaatta gggagccata aaataaagtt ccataaaaaa
    20101 atcctgtttt atagtattca gcttttatat gcccccaacc aggaataagt gctgatctcc
    20161 aaacatatcc ccatttagag atgaattcaa ttttttcttt ttctgttttt ttagaatcgt
    20221 tcgctatatc taaattttca tttgtagatt ttgtttctt
//
LOCUS       DAOQOS010000100        20030 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_1326_length_20030_cov_5.315344, whole genome shotgun sequence.
ACCESSION   DAOQOS010000100 DAOQOS010000000
VERSION     DAOQOS010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 20030)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20030)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20030
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1326_length_20030_cov_5.315344"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..336)
                     /locus_tag="PLF66_18745"
     CDS             complement(<1..336)
                     /locus_tag="PLF66_18745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004497232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HMZ66136.1"
                     /translation="MKDKIKITEVGPRDGLQNETKVIKTEDKLQYIQLLKKAGLKNIE
                     ATSFVRADKIPQLSDAIELSKNLNFNDDVVYSALTPNIKGYESAVASGFKEVAIFTAA
                     SESFTKKNIN"
     gene            complement(338..1318)
                     /locus_tag="PLF66_18750"
     CDS             complement(338..1318)
                     /locus_tag="PLF66_18750"
                     /inference="COORDINATES: protein motif:HMM:NF024911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HMZ66137.1"
                     /translation="MEEKNEIGLVLGKFYPLHKGHIHLIQSARKKVNKLYVLVCSLKR
                     EAIPGDLRFYWMKETFPDLDVYHLTDENPQFPEEDPNFWEIWKRSIRQFIKEPIDIVF
                     TSEEYGEMLAKKLNARHICIDLERKTFPISGTQVRESPFKNWDLISDAAKSYFVKRIV
                     IYGPESTGKTTLARTLAEYYKTEWVPEFAREYLENKNSPVELSDIPIIAEGQIRTEDE
                     LAKKANRILICDTDILVTKVYSEHYYNTCPEWIKEGAYNRKYALHLLTNNDIPWVADI
                     LRDRGDRREEMFELFKTELEKANKSYKLISGNYKERFDTAVQIIDELLGY"
     gene            complement(1291..1875)
                     /gene="pnuC"
                     /locus_tag="PLF66_18755"
     CDS             complement(1291..1875)
                     /gene="pnuC"
                     /locus_tag="PLF66_18755"
                     /inference="COORDINATES: protein motif:HMM:NF016837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HMZ66138.1"
                     /translation="MNELFNLILDLENAGVLFGLISVILTVRESIFSWYFGIINVILF
                     GILFYNSKIYGQMILQIFFLFVSIYGLYKWKYGGKNGLELESRNISQLEQIISFFFIL
                     LLSGLIFIAINFVSSKEKFSFLDAIITAISFIAQFLQARKVLESWILWIVVDILSIFL
                     FYQSSLYKVGFFYFILLILATTGYFTWKRKMKSA"
     gene            complement(1872..3251)
                     /locus_tag="PLF66_18760"
     CDS             complement(1872..3251)
                     /locus_tag="PLF66_18760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66139.1"
                     /translation="MIPEEIKSRLPLIQEENLPLLYKLKEHEHAPNWNYTCGDRLTKE
                     DVIQINHFAERLRRERDKIQKDVPEEVLSWILDLKEFVLIFKENIKNLDIKKDFEKIR
                     CMSREDIAKGLENIIPTNVSLDRLIVNSTSGTTGHPIITPNHPVAIGAYTPLLLYALE
                     KHKVKPVFTKDNVGCMLVCAQKETAVYSTIVPMLNGTGFAKINLDESSWKTKESCQAY
                     IEEFSPEILTGDPFSFAELINIQFNNYNPKAIVSTSMEMSPSLRKKLEDRFKVPVIDY
                     YSLNETGPIAYSCPIHSEEFHILPTDIFIEITDLNGNRLSEDTSGDITITGGRNPYIP
                     LLRYKTGDRAKIKWNQCECGESTPRLYDLETRKQVLFYNKDHVRINPIDITRILKRYP
                     ILQYEFKQLKDLKCNLKIRKLYDFTFDQEENLKKDLLMLFKSKIDINIDFDLSLDRKP
                     IPYSSELDI"
     gene            complement(3248..3847)
                     /locus_tag="PLF66_18765"
     CDS             complement(3248..3847)
                     /locus_tag="PLF66_18765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66140.1"
                     /translation="MDNGPDLYILMQRLQNCPEEFFWSPAVANPPKNYTGKILTHAIV
                     NDLLQGLGLVTDPIEIYELFNLKYSEANINYLQIILISAYLLHYEWFLNAQKYGEKAK
                     ILLSQKLKKISEIVNAKDFVMDSERREELIRFCLKELDLKPNGETDIHSIDRLTTINS
                     IERQRVIEESKAAQKRAQEIREAIARKEAEEAASKWNRE"
     gene            complement(3840..5585)
                     /locus_tag="PLF66_18770"
     CDS             complement(3840..5585)
                     /locus_tag="PLF66_18770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HMZ66141.1"
                     /translation="MTKQKIPLESLEKLKLKWLSFWPEAIEIWSKYLKLSDPRFCLTK
                     EEENKEHLLSSFAMIRLDDHAVVISLRQVAQYELEDYPLEIMCHEIGHHIYCPADLTD
                     HGRLIARIRRAIPGYESEAPLIANLYGDLLINDKLKREHGLRLDDVYKKIGGDSVDKM
                     WTFYLRIYEILWGLTKNTLAKGEITSIMEGDAQLGNRIIRNFSNDWLEGAGRFASLCY
                     PYLVEEGTSKIRSIMSPLLDSQETGSGSTFPSGLSEIEEGELSGAKHPSLDGEIKENN
                     AGEDHTKVGNAAGQYREPFEYGAILKALGLTMSQEEITIQYYKERSMPYLIPFPTKET
                     PVSKEPLMEGLDIWDIGSPFENIDWFQTAMRSPVVIPGFTTVEQIFGVSEGSLPEKEP
                     VDLDIYIDCSGSMPNPGYNISYLALAGTIITLSALRVGSRVQATLWSGPKEFYTTNGF
                     ISDEKELLKVVTGYLGGSTAFPIHILRETYTDRKPNARKVHILVISDEGVTTMFDKDE
                     KKNSGWDISEMSLKNCGGGGTFVLNLYSDWQTDKSLVRANQQGWDMHRVNDWEALVKF
                     AKEFSNKKYGSEANG"
     gene            complement(5578..6624)
                     /locus_tag="PLF66_18775"
     CDS             complement(5578..6624)
                     /locus_tag="PLF66_18775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66142.1"
                     /translation="MATEQIKNPILIEILKENREKLNSLFGIYKLNYPAIEKDILFFY
                     LVFMVEPLFEKNQNLKKETLTSTFLILYEKLLEMIGKNLLGNSGRYPFFENKIILCLE
                     NSGKWIFEDPENFISSTANAILNIGNITPKGLEKWTSFYLKILKTANSLNEIFLAGKV
                     AAWLSGMAQYRNESLQIMRQIDINLLEILVGNSNVRHLNREKMIDRLEKDPWLDLENA
                     IKENSGNRKIRVSRISGFTGLGGHFISPPKVEYLEDHFIVNDGVNYYILFLDIFGSFL
                     LRISFEFYNELKKKSFVNKEISISKEGLVQFANHSISYPPLKNYSHSASSQSTICATS
                     PYSYTIYAIGLRND"
     gene            complement(6606..8192)
                     /locus_tag="PLF66_18780"
     CDS             complement(6606..8192)
                     /locus_tag="PLF66_18780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015677809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HMZ66143.1"
                     /translation="MATTDRTQKVSESPTLPKDRSGKNKFDIADLYHGTLSGGQTSAE
                     ENSESGLDEKLRKAYLWIVNNAIISPYYDIEYNKGANQSYTIGDLKSRVTFPSDQSYS
                     SFVLLPLLNLITRRRCLIIGGPGRGKTASAMLMGVIAGYPLKEIKRSIQHGQPQMTIS
                     DLLGNPIPSDLMTAKDMDDIKISWRKWLGMRIKIIDEYNRIPTRTQSALLTVLGDNYA
                     EILDQIYECPEAAWYLTANDDAGGGTYQVIEALRDRIDIVVKALHFNSRFIGDLLYRI
                     EHGIKPEESVPRQIIFTEEEINQMNKEILEVVLPEKLLKRIEFFSSHFELYESASEQL
                     EYKTKDNVKVSGVDFRSIASAETGKDKLKDIGSQTRNGLSVRSIMTSLIFIKALAYFR
                     GNKEVEFDDVRHILPFVFHDKLVQNSDSPFFDQPGNTVYRVDRVSWIRKLFDMSCAEY
                     DRLNLDKKDPIDELEKKFNQGLEGVSEKEVEEELRKIARLMEDWSKNRKLYGHVFDDV
                     LKLKYLHQRYSNYLRWLQNK"
     gene            complement(8194..9984)
                     /locus_tag="PLF66_18785"
     CDS             complement(8194..9984)
                     /locus_tag="PLF66_18785"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HMZ66144.1"
                     /translation="MKLQKNFLQKIFSTIKRPFFLLVLFIIFILSFQCSSSEKKTFPE
                     KAYDEEKGSSSPMSPSDSDRKKSKELDKKNPKEEKTVDAKPTSVSEKSESGLKAGYAD
                     DNKQFNLFLNFLDKFKSSAAHIDLDVKERIIFFVKDKNSRSIPNASIQILANNKELTT
                     GTTYSDGSFLFFPSLYNDVYKFKARITKDNSKTEIEFDRQGARTKEVILDYPQAKENE
                     VPVDIVFVMDTTGSMGEEIQRLKSTIEIINLNLTGNSKNEIRFGMVLYRDKKEEYVTK
                     VIPLTNDLENFKKELNKVSAGGGGDYPEDLQSALKDTIKEIDWRKNAVKLTFIITDAP
                     PHLDYKQEYTYISAMKEASERGIKLYSIGTGGLDINGEYVLRQISQFTNSKYIFLTYG
                     EKGESEGGASGSVSHHTGANFPTDKLETIVIRFAKEEIQAAMGKPLANGEDYFTANKI
                     DSEKKEETLNKLFDQAITQLVDYSTFSIKTGTTVGILPFAVENSSLNKNGEYFSEQMV
                     LGFSRNKTFKIVERKDLQKVLAEWKLNLQAVDESNAIKVGKLLGANLLVNSKIFKKGS
                     SYEIYVKMINSETGEIQSATKLIVDTKLGL"
     gene            10052..10855
                     /locus_tag="PLF66_18790"
     CDS             10052..10855
                     /locus_tag="PLF66_18790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016759632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HMZ66145.1"
                     /translation="MNILNINSFSNEKVKYVTKLKEKRFRDRERKFVIEGFRELAKAQ
                     SSGKIKFETLFISPECFLGVNEEELIKKINSKIVELPKKIFEKISYRDRPDGLLAIAE
                     TPDFGFDYTGDRKGGLFLIIEGVEKPGNLGTILRTADGAGVSAVFVTDARIDLFNPNV
                     IRASTGTIFTLPTYIAEIEKLIEYFKKYKVQIIALSPEGKTNYLDKNYKARTALLFGN
                     EQYGLSPYAKENADSLVSIPMQGESDSLNLAMSCGILVYEVLRQKMQRI"
     gene            10867..11922
                     /locus_tag="PLF66_18795"
     CDS             10867..11922
                     /locus_tag="PLF66_18795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HMZ66146.1"
                     /translation="MNIAYYVSGHGFGHISRSYEIILALLEHKKVDKIFVNTTRKDFV
                     KDKKENLVFREIATDVGIIQEKSISMDLEKTIDAIEKLQKNKKEIIESEIEFLKKEKI
                     DIVFSDSSSLPFAFANKLNIPSYFIGNFTWDFIYQNYTKQYSFFKDYVKLIRDEYSLA
                     TFGFILPFHCPMDYIPRKKNIGIIGRSPKRNRKEIRESIRFSEQNQYFLFSFGAYGIP
                     ESDLSYMGLKENQWIVVSGYEGLKGNKVMDVKDIYYPDLVRACDYVLTKPGYGILSET
                     YLAGTPIIYTDRGDFAEYDYLVQAMETYHYSAFLSQKELLSMNLESAVQKIEIQRNSR
                     KIPYLSKGEIEILEQIF"
     gene            12030..12344
                     /locus_tag="PLF66_18800"
     CDS             12030..12344
                     /locus_tag="PLF66_18800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66147.1"
                     /translation="MLKKLGILILITSFLAVANVFAQEEESDTTEPKEEMTDTKDAKE
                     AKEAKTSKTEKGEKKGNKKGAKKGNKKGGKKAGKKGGKKGNKKAPNAETKTETPDAEE
                     TK"
     gene            complement(12397..13809)
                     /locus_tag="PLF66_18805"
     CDS             complement(12397..13809)
                     /locus_tag="PLF66_18805"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HMZ66148.1"
                     /translation="MKSKILLLIISLFLSFFSSNSIFSEEDNLNIPPKALPLSPIRIS
                     SYQTFKLENGLTVFLIEDHKLPTVSFVLYFDFLTKIKKTKYGLGELTTRLMKEGAGKR
                     SKQQLDEELDFMGTDLVIQNNLIYASGLEKYSKESLEILSDIVKSPAFDKNEFQKIKE
                     EIIAEIKSNKLDPNSEANNLMKTLLYPENHPYREIPSEESMQNIKIEDCKAYHNKFFK
                     PGNSYLAIIGDISLDKAKTLVESSLGNWSSRKVKDVKIPALPKEELTKIFLLDREDSV
                     QSVIKISKLIDLKPNDKNLFSVIVMNTILGGGTFRLYENLREKNGYTYGVYSSLQPDR
                     IAGNFSIDLSTKNNVTHKSIEEIFSELERIQKTKVDEKELQLVKNYLTGQFSLSLENP
                     LTLALFAIKTAIYHLPADFYDKYLENIRKVTAEDVQKAANHYIPQNKMKIVVVGKKKE
                     ILKDLNKLKDFKIVETNTTK"
     gene            complement(13809..15128)
                     /locus_tag="PLF66_18810"
     CDS             complement(13809..15128)
                     /locus_tag="PLF66_18810"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HMZ66149.1"
                     /translation="MKKIYLIALLFVISLQATEKFNINFEKFKLTNGLTVILHRDNTR
                     PIVSVGFLYHVGSKDESPNRTGFAHFFEHLMFEGSKNIKRGEFDKLILNAGGESNAHT
                     TFDQTYYEASIPSNQLPLILWMESERLLHLKINPEGVETQRKVVKEERSERLDNVPYG
                     DLIEILHQMSFRKHSYKWLPIGSNQYIDKATLEEFNHFHKKYYTPDNGVLVIAGDIDS
                     KSTKELVEKYFNRIPKSKKKLKRNKVIEPPLKKEIQKTVYANVEFPSLTYAFHAPNQG
                     TNDYYPFVFVDLILSGGESSRLVYNLKTKNNLIINVEATYRAMEDYGLYSITSYAKDK
                     SSYEKVPANLEQELKRLQTEFVSDRELEKVRNQIQKNIYATNANLAGIAENLAEYETF
                     YGNASLINTEYEKVLSIKKEDIQRVAKKYFTLDKKIILHYLPNKYKK"
     gene            complement(15125..17416)
                     /locus_tag="PLF66_18815"
     CDS             complement(15125..17416)
                     /locus_tag="PLF66_18815"
                     /inference="COORDINATES: protein motif:HMM:NF013236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HMZ66150.1"
                     /translation="MSEETLSKKDIEALLEATTRKEDKPLYDKIHSDPFSVLNNDYWM
                     REASIEILENLKMENFKNFTKKLSDKLSASERNLFKEILSAILSEVIKSKKFGDIELG
                     KKIGFEIVPAKKIRKQFKPGMQCFYGNFLGRIQGIYSILLSNDTTVRIVKALDKGEMK
                     VGNNHLKNIGDWLYSVVCSFMEALTLKIGEVKSSEMYQALVTKSDQLILPEGSNYFKI
                     EFSMVTSGEVFNFHFLFSVYNAKNILNYIIQLNPNVKKNLKSHHRMIRSIESKLEKFM
                     KNRETYLSENESNEILNYINKNLSKEEKIFLLSPIKERGEKPDIEASLIKVFTSLSNE
                     IIDRRYKLNEAEKERKGKLYSLISSIPFPVNVRFEDEIFDYRSFEQKTDKNTNIEKFI
                     GKYGTLIFSNKPLQEILLKKENEKFLLYDLTGNVIPSKYELHLANTELMNLEIEADIE
                     IGKAKLPLDRLINIKPGNIIELNKDINDPVYLVIENVVFAKTTVVVSNEKFGIWIEDI
                     SNRNDLKSKSLEGVLPSSIESQTKIPMADIRVILGKLKLTIKDLLTMSKGSIIELDKS
                     IFEPVQILVGYDLFFPGEIVVLENGKFGVRFVNNVGLEQKHNSEIIDLDKEPDVYNFQ
                     PNQSSNQNPFEFLERLESNTIAGIIQSEQAQMISMILSFLSAEKSASILSLLPESIQT
                     DVVAKIAISQKANIEIAREVAKILEKKVASLIHDTYANAPGFDSIVKILNSTDKEKRD
                     KMIASLEKSFPDIYNILKSNTVK"
     gene            complement(17416..18354)
                     /locus_tag="PLF66_18820"
     CDS             complement(17416..18354)
                     /locus_tag="PLF66_18820"
                     /inference="COORDINATES: protein motif:HMM:NF014235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66151.1"
                     /translation="MDEILTNDELDALLTAISTTSLEKSDDAPVEPKKIRTYDFKKPD
                     KFSKDHLKSFQYIFETFSRIASPALSLKFEIPLSINFSSIDQLSYEEFSKLIPENSIL
                     GMVNLKPLHGYTIFEFEPEFTFSLIDILTGGKGDPIIKPRQLTDLELSIIQTIMHTLV
                     SYLKESFQSVLQIKPVLEKIETNTFFKQKLYSGEIVLQVCLDLNLKGTEYSMNICLPY
                     TSLEPVIHKFSNKILDYIDDSNKDNSQYERRKKILESCMIRTYSEFQAGTVYIQPNGE
                     TEDISHEVNINLTHDSKSSHYFKTLFKNFIRSAEVL"
     gene            complement(18455..>20030)
                     /locus_tag="PLF66_18825"
     CDS             complement(18455..>20030)
                     /locus_tag="PLF66_18825"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HMZ66152.1"
                     /translation="KTEKKTQININSDSKNDTYVFINVLMGDIKNPIGVAGVSIKFNQ
                     VAKEFTETDPEYDARVWLIDNQGNVKIASDVSGINQPIGKFTSKEIEKSILKDPSKVS
                     VMEYSNDKGLTDIVHVPLEINDWGVVYEVPRSKMTGVLRNIAYGTFAVCVVSILFVFI
                     IFYYGTHSITNPIQSLVKALNSISSGEINQKITVKSEDEIGHLATNFNLFTERLMTIT
                     KSVKESSLLIAASSREMSDTTKTYSMNAQSQASTIEEITASIEQLSERVSDVTNHTDI
                     QFNNLNALSGKLKELSGIIEEMNEIIQKTLGNTKAISIEAMSGEEALRSMNVSMTQIV
                     ESSQDMKNIIQIINDISEKINLLALNASIEAARAGVAGKGFAVVALEISRLADQTASS
                     VKGIDLLIKKNNKQILEGIDTVNVIMKKTNTINSGVESIVEKMNAIFDYMQKQISTKF
                     LVERETEIVKSRANEIQQITKEQKVAFDEIVKAITFINEISHSNTESSNVIATKSLEL
                     SKVAEMLKTKVDFFKF"
BASE COUNT         6303 a         3353 c         2978 g         7396 t
ORIGIN      
        1 atttatattt ttttttgtga aagattcgga agcagcagta aaaattgcta cttctttaaa
       61 tccggaagct actgccgact cataaccttt tatgtttggg gttaatgccg agtagactac
      121 gtcatcgtta aaatttaaat ttttagataa ttctatggca tctgaaagtt gaggaatttt
      181 atctgctcga acaaaagaag tagcctcaat atttttaagt cctgcttttt ttaataattg
      241 aatgtattgt aatttatctt ctgtttttat aactttagtt tcattttgta gcccatctct
      301 cggtccaact tctgtaattt ttattttatc tttcattcta atatcctaat aattcatcta
      361 taatctgaac ggctgtatca aatcgttctt tataattccc actgattaat ttatatgact
      421 tatttgcttt ttctaattct gttttaaata attcaaacat ttcttccctt ctatcgcctc
      481 tatccctaag aatatctgct acccaaggaa tatcattatt tgttagaagg tggagtgcat
      541 acttacgatt atacgctcct tcttttatcc attcggggca agtattataa taatgttcgc
      601 tatatacttt tgttacaagt atatctgtat cacaaattaa aattcgattt gcctttttcg
      661 caagttcatc ctctgttctt atttgtccct cagctatgat cggaatatca cttagctcta
      721 ctggagaatt tttattttct agatactctc gtgcaaattc aggcacccat tctgttttat
      781 aatactcggc taacgttcta gctaatgttg ttttgccagt agactctggt ccataaatta
      841 caattctttt tacaaagtaa ctttttgcag catcggatat taaatcccaa tttttaaatg
      901 gactttctcg aacttgagtg cctgagatcg gaaatgtttt tctttccagg tcaatgcaga
      961 tatgccttgc atttaacttt ttagcaagca tctctccata ttcttctgaa gtaaagacta
     1021 tatcaattgg ttcttttata aactgcctta tactcctttt ccatatttcc cagaaattcg
     1081 gatcttcttc tggaaactgt gggttttcat ccgttagatg atatacatct aaatccggaa
     1141 atgtttcttt catccaataa aatcttaaat cccctggaat tgcttccctt tttagagaac
     1201 aaactaatac atatagttta tttacttttt tacgagcaga ttgaattaga tgaatatgac
     1261 ctttatgaag tggatagaac tttccaagca ctaagccgat ttcatttttc tcttccatgt
     1321 gaagtatcct gtagttgcaa gtatgagtag aataaaataa aaaaagccaa ccttataaag
     1381 tgaagattga taaaaaagaa aaatcgaaag aatatcaaca actatccaaa gaatccaact
     1441 ttctaaaact tttcttgcct gcaaaaattg agcaataaaa ctaatggcag tgattatcgc
     1501 atctaaaaaa ctgaactttt ccttcgaaga aacaaaattg attgcaataa aaatcaaacc
     1561 acttaagaga agtataaaga agaatgaaat gatttgttct aattgagaaa tatttctaga
     1621 ttctaactct aaaccattct ttccaccata tttccatttg tataaaccat aaatactcac
     1681 aaataaaaag aatatttgta gaatcatctg tccgtaaatt ttagaattgt aaaaaagaat
     1741 tccgaagagg ataacattga ttatcccgaa ataccaagag aaaatgcttt ctctaacagt
     1801 tagaattaca ctgattagtc caaataatac tcctgcattt tctaaatcaa ggattagatt
     1861 gaataattcg ttcatatatc taattctgaa ctgtatggta ttggtttgcg atctaggctt
     1921 aagtcaaagt caatatttat atctatttta ctcttaaata acattaatag atcttttttt
     1981 agattttctt cttgatcaaa agtaaaatca tacaatttac gtatctttaa attgcatttt
     2041 agatctttta actgtttaaa ttcatactgt aatatcggat atcgttttaa gatgcgagta
     2101 atatcaatgg gattgattct aacatgatcc ttattgtaaa ataaaacttg ctttctagtt
     2161 tccaagtcat aaagcctagg agtagattca ccacattcac attgattcca ttttattttt
     2221 gctctatctc ctgtcttata acgaagaagt ggaatataag gatttcgacc tccggttata
     2281 gtgatatctc ccgatgtatc ttccgatagc ctatttccat ttaggtctgt tatttcaata
     2341 aaaatatctg ttggtaaaat atgaaactct tcagagtgaa ttggacaaga ataggcgatc
     2401 ggtccagttt cattcaaaga ataataatct atcactggaa ctttaaatct atcttccaat
     2461 tttttacgga gggacggact catttccata gaggtggaga ctatcgcttt tggattgtag
     2521 ttattaaatt gaatgtttat caattcagca aaggagaagg ggtctcctgt taatatttct
     2581 ggagaaaatt cctcaatgta agcttggcag ctttctttcg ttttccaaga actttcatct
     2641 agattaattt tcgcaaaccc agttccgtta agcataggaa cgatagtaga atacacagca
     2701 gtttcttttt gagcacacac gagcatacaa cctacattat cttttgtaaa gactggtttt
     2761 accttgtgtt tctctaaagc ataaagaaga agaggggtat aggcacctat cgcaacagga
     2821 tgattgggag taatgatagg atgacctgta gttcctgaag tagaatttac aattagtcta
     2881 tcaagagaga cgttagtagg gataatattc tctagtcctt ttgcaatgtc ttctctagac
     2941 atacacctaa ttttttcaaa gtctttttta atatctaaat tctttatatt ttctttaaat
     3001 atgagcacaa attcttttaa atctaatatc caagagagaa cttcttctgg cacatctttt
     3061 tgaattttat ctctttccct ccttagcctt tctgcaaaat gatttatctg aatcacgtct
     3121 tcttttgtaa gtctatcccc acatgtgtaa ttccaatttg gggcatgctc atgctctttc
     3181 agtttataca aaagaggtaa attttcttcc tgaatcagcg gtagccgaga tttaatttcc
     3241 tcaggtatca ttccctattc catttagaag cggcttcctc tgcttctttt cttgcgatgg
     3301 cttctctgat ttcttgtgct cttttctgtg ctgccttact ttcttctatt actctttgcc
     3361 tttctatgga gtttattgtt gttagtctat caatagaatg aatgtccgtt tctccatttg
     3421 gttttaaatc caattctttt aagcaaaatc gaatcagttc ttctcttctt tctgaatcca
     3481 ttacaaaatc ttttgcattt acaatttcag atattttttt taatttttga gatagtaata
     3541 tttttgcttt ctctccatat ttttgagcat ttagaaacca ttcataatga agtaaatatg
     3601 ctgatattag aattatttgc aggtaattaa tatttgcctc tgaatattta agattaaata
     3661 attcataaat ttcaatagga tctgtgacta atcctaaacc ttgtaataga tcatttacaa
     3721 ttgcatgcgt cagtattttt cccgtataat tctttggggg atttgctaca gcaggggacc
     3781 agaaaaattc ttctggacaa ttttgtaaac gctgcatcaa aatatataaa tctggtccgt
     3841 tatccatttg cctcactccc atatttttta ttactaaatt ctttcgcaaa ttttacaaga
     3901 gcttcccaat cattcactcg atgcatatcc caaccttgtt gatttgcccg caccaatgat
     3961 ttgtctgttt gccaatcgct gtataaattt aaaacaaagg ttccaccgcc accacaattt
     4021 tttaaactca tttcggaaat atcccaacca gaattctttt tttcatcttt gtcaaacatg
     4081 gtagttacac cttcatcaga tattactaaa atatgaactt ttcttgcatt tggttttcga
     4141 tctgtatacg tttctcttaa aatatgaata ggaaaggcag tcgaaccacc cagatagcca
     4201 gttacaactt ttaatagttc tttttcatct gaaatgaatc cattagtagt ataaaactct
     4261 ttaggaccac tccatagggt tgcttgcaca cgggaaccca ctcgtaaagc ggataacgtt
     4321 ataatagttc cagccaaggc taaataagaa atattataac ctggatttgg catagatcca
     4381 gagcaatcaa tataaatatc caaatcaaca ggctcttttt cgggtaacga tccttctgaa
     4441 actccaaata tttgttctac tgttgtaaaa cctggaatta ctactggact acgcatcgcc
     4501 gtttgaaacc aatcaatatt ttcgaaagga gatccaatat cccaaatatc aagtccttcc
     4561 attaacggct cttttgaaac cggagtctcc ttagtcggaa aaggaatcaa ataaggcata
     4621 gatctttctt tgtagtattg gattgtaatt tcttcctgac tcatagtaag tcccaatgct
     4681 ttaagaatag ctccatattc aaatggttct ctatattgcc cagctgcatt tcctactttt
     4741 gtatgatctt cccctgcatt attttctttt atttctccat ctaatgatgg atgttttgct
     4801 ccagataatt ctccttcttc gatttcagaa agccctgaag gaaaggtaga tcctgatcct
     4861 gtttcttgag agtcaagaag gggactcatt atacttctaa ttttacttgt tccctcttct
     4921 actaaatagg ggtaacaaag ggaagcaaat cttccagcgc cctctaacca atcattcgaa
     4981 aaatttctaa taatgcgatt tcctaactgg gcatcgccct ccataatcga tgtaatttca
     5041 cccttagcga gggtattctt tgttagcccc caaagaattt cataaattct taaataaaaa
     5101 gtccacattt tatcgacgga atcaccgcct atttttttat atacatcatc taatctcagt
     5161 ccatgttctc tttttagctt gtcattaatt aaaagatcgc cataaagatt tgcaattaaa
     5221 ggagcttctg attcatatcc aggaattgct ctgcggatac gggctattag cctcccgtgg
     5281 tcagtaagat cagctgggca atatatatga tgaccaattt catggcacat aatttctaaa
     5341 ggataatctt ctaattcata ctgagcaact tgtctcaaac taattaccac agcatgatca
     5401 tctaaacgta tcatagcaaa gctacttaaa aggtgttctt tattttcctc ttcttttgta
     5461 agacaaaatc taggatcaga aagctttaag tatttactcc atatctcaat ggcttctggc
     5521 caaaatgata accacttcaa ttttagtttt tccaaagact ctaatggaat tttttgttta
     5581 gtcatttcta agtccaatgg catagattgt ataggagtag ggacttgtag cacatatcgt
     5641 actttggctc gatgcactat ggctataatt tttcaatgga ggatatgaaa tcgaatgatt
     5701 cgcaaattgg actaatcctt ctttggaaat ggaaatctct ttgtttacaa atgatttctt
     5761 tttcagttca ttataaaatt cgaaagaaat tcttaacaga aaagatccga aaatatctaa
     5821 gaataatata taataattca caccatcatt tacaataaaa tgatcttcta aatattctac
     5881 tttgggagga gaaataaaat gtccaccaag cccagtaaag ccgctaattc gactaacacg
     5941 gatttttcta ttccctgaat tttctttaat ggcattttct aaatctaacc aagggtcttt
     6001 ttctaatcta tcgatcattt tttctctatt taaatgtcta acgttagaat ttccaactaa
     6061 tatttcgagg agattaatat caatttgcct cattatttgt aaactttcat ttctatattg
     6121 cgccattccg gaaagccaag ctgcaacttt tcctgctaaa aaaatttcat ttaaactatt
     6181 agcagttttt aatatcttta aataaaaaga agtccatttt tctaaacctt taggagtaat
     6241 atttcctata tttaaaattg catttgctgt tgaacttata aaattttctg gatcttcaaa
     6301 aatccattta ccagaatttt ctaaacagag aatgatttta ttctcaaaaa aaggatatct
     6361 cccagaatta cctagtagat tttttccaat catttctaga agtttctcat ataagattag
     6421 aaatgtagaa gtaagcgtct cttttttgag attctgattt ttttcaaata agggctcaac
     6481 cataaacacc aaatagaaaa ataaaatatc cttttcaatt gcaggataat ttagtttata
     6541 aattccaaat agggaattga gtttttccct attttctttc agaatttcaa ttaatatagg
     6601 attttttatt tgttctgtag ccatcttaga taattagaat acctttggtg taaatattta
     6661 agttttaaga catcatcaaa tacatgtcca tataatttcc tgtttttact ccaatcctcc
     6721 atgagtcttg caatttttcg caattcttct tctacttctt tttcggaaac accttcgagc
     6781 ccttggttaa atttcttttc gagttcgtct atcggatctt tcttatcaag attgagtcta
     6841 tcatattcag cacaagacat atcaaaaagt tttcgaatcc agctcacgcg atctactcta
     6901 taaacagtat ttccaggttg atcgaaaaaa ggagaatcag aattttggac taatttatca
     6961 tgaaatacaa aaggaagtat atgtctaacg tcatcaaatt ctacttcttt atttcctcga
     7021 aaataagcaa gggctttgat aaaaataaga gatgtcataa tggaacgaac agatagtcca
     7081 tttctagttt gactccctat atcttttaat ttatcttttc ctgtctctgc agaagcaatc
     7141 gatctaaaat caacaccaga tacttttaca ttgtcttttg tcttatactc taattgttcg
     7201 ctcgctgatt catacaattc aaaatgacta gagaaaaatt caattcgttt gagtagtttt
     7261 tctggaagaa ctacttctag aatttcttta ttcatttgat tgatttcttc ttctgtaaat
     7321 ataatttgcc taggaactga ttcttctggt ttaattccat gttcaattct ataaagtaaa
     7381 tctccaataa atctagaatt aaaatgtaaa gcttttacaa ctatatcaat tctatctctt
     7441 agggcttcaa ttacttgata agttcctcca cctgcatcat cgttagccgt aagataccaa
     7501 gctgcttctg gacattcata gatttgatct aatatctctg cataattatc tcccagaact
     7561 gttaaaagag cagactgagt tcttgttggt atgcgattat attcatcaat aattttaata
     7621 cgcataccaa gccattttct ccaagagatc ttaatatcat ccatatcttt ggctgtcatc
     7681 aaatcagagg gaattggatt cccaagtaaa tccgatatag tcatttgtgg ctgaccatgt
     7741 tgaattgatc gtttaatttc tttcagcgga taaccagcaa taacccccat taacattgca
     7801 cttgctgttt ttcctcgtcc aggtcctcct atgattaagc atcttcttct agtaattaga
     7861 tttagaagtg gaagcaatac aaaactagaa tagctttggt cagaaggaaa ggttactcta
     7921 cttttcaaat cgcctatcgt ataggattga tttgcgcctt tattgtattc aatatcataa
     7981 taagggctaa taatcgcatt atttacaatc cataaatagg cttttcttag tttttcatct
     8041 aagcccgatt ctgaattttc ttccgctgaa gtctgtccac cagagagagt tccgtgatac
     8101 aaatctgcga tatcaaattt attttttcca gacctatctt tgggaagagt aggtgattct
     8161 gatacttttt gtgttctgtc tgtagttgcc atattataat cctaattttg tatccactat
     8221 caattttgtt gcggattgaa tttctcctgt ttctgaattt atcattttaa catagatttc
     8281 ataagaagag cccttcttga aaattttact attaactaat agattagcac ctaaaagctt
     8341 tccaacctta attgcattgg attcatcgac tgcttgtaaa tttaacttcc actccgctaa
     8401 aactttttgc aaatccttac gctcaacaat tttaaaagtt ttatttcttg aaaaacctaa
     8461 aaccatctgt tcactaaaat actcaccatt tttatttaaa gatgaatttt caacggcaaa
     8521 tggtaatata ccaacagttg ttccagtctt tatagagaaa gtagaataat ccaccagttg
     8581 agtaattgct tggtcgaaaa gtttatttaa tgtttcttct tttttttcgg aatcaatttt
     8641 atttgcagta aaataatctt ctccatttgc tagaggtttt cccatagctg cttgaatttc
     8701 ttccttagca aatcgaatta cgattgtttc tagtttatcc gttgggaaat ttgccccagt
     8761 atgatgacta acgctaccag atgctccacc ttcactttca cctttttcac cataagttaa
     8821 aaatatgtat ttcgagtttg taaattggga aatttgtctt agaacatatt ctccattgat
     8881 atcaagcccg cctgttccga tagaataaag tttaattcct ctttcgcttg cttccttcat
     8941 ggctgatatg tatgtatatt cctgtttata atctaagtga ggaggggcat ctgttatgat
     9001 aaatgtgagc ttaactgcat tttttctcca gtctatttct tttatcgtat cttttaaagc
     9061 tgattgcaaa tcctcaggat aatcgcctcc acctccagca gacactttgt ttaattcttt
     9121 tttaaaattt tctaaatcat ttgtgagagg tattactttt gttacatact cttctttttt
     9181 atctctataa agaaccattc caaatcgaat ttcatttttt gaattaccag ttaaatttaa
     9241 gttgataatc tcgattgtgc ttttgagtct ttgaatttct tctcccatac taccagttgt
     9301 atccattaca aagactatat ccacaggcac ttcgttttct ttagcttgcg gataatctag
     9361 aataacctct tttgttcttg cgccttgtct atcaaattca atttctgttt ttgagttatc
     9421 ctttgtaatt cgtgctttga acttataaac atcattataa agagatggaa aaaaaagaaa
     9481 tgatccatct gaataggtag tccctgttgt tagttcctta ttatttgcta aaatttgaat
     9541 ggaggcattt ggtatacttc tagaattttt gtcttttaca aagaaaataa ttctttcttt
     9601 tacatccaaa tcgatatgtg cagcactaga tttaaactta tctaaaaagt ttaaaaataa
     9661 attaaattgt ttgttatcat ccgcatatcc tgcttttaat ccagactcag acttttcgga
     9721 tacacttgta ggtttcgcat ctaccgtttt ttcttctttt ggattttttt tatctaattc
     9781 ttttgacttt tttcgatcag agtcagaagg actcatcggt gaagatgacc ctttttcctc
     9841 gtcatatgct ttttctggga aggttttctt ttcggaagag gagcattgga atgagagtat
     9901 gaaaataata aaaagtacca aaagaaaaaa aggtcttttt atcgtagaga atattttttg
     9961 tagaaagttt ttttgtagtt tcatgattta tccgatagat ataacaaata aggaaagggt
    10021 ttttgtttat tcttaaattt tagttataac aatgaatata ttaaatatca acagtttttc
    10081 aaacgaaaaa gttaaatacg taactaaatt aaaggaaaaa cgatttagag acagagaacg
    10141 taaattcgta attgagggtt ttagggaatt agcgaaagca cagtcctcag gtaagattaa
    10201 atttgaaact ctctttattt ctcctgagtg ttttctaggt gttaacgagg aagaattaat
    10261 taaaaaaatt aactctaaaa tagtagaact tcctaagaaa attttcgaaa aaatttccta
    10321 tcgagataga ccagatggct tacttgccat tgcggagact cctgattttg gctttgatta
    10381 tacaggcgat aggaaagggg ggttgttttt aatcattgag ggtgttgaaa aacctggaaa
    10441 tttaggaaca attcttagaa ctgcagatgg agctggagta tcagcagtat ttgtcactga
    10501 tgcaagaata gatttattta atccaaatgt aattagagcc tcaactggaa ctatttttac
    10561 tcttccaacg tatatagcag aaatagaaaa attaattgaa tactttaaaa aatacaaagt
    10621 gcagataatc gctcttagcc ccgaagggaa aacaaattat ttagataaaa actataaggc
    10681 aagaactgcc ttgttgtttg gaaatgaaca atatggatta tccccatatg ctaaagaaaa
    10741 tgcagactca ctagtttcta ttccaatgca aggagaatct gattctttaa atttagctat
    10801 gtcctgcggt attttagtat atgaagttct aagacaaaaa atgcagagga tataagggat
    10861 ttcaatttga atatagctta ttatgtaagc ggacatggct ttgggcatat tagccgatct
    10921 tatgaaataa ttttagctct cctagaacat aagaaagtag ataaaatttt tgttaatact
    10981 acaagaaaag atttcgtaaa agataaaaaa gaaaatttag tctttcgaga aattgctaca
    11041 gacgttggaa ttatccaaga aaagtctatc agtatggatt tagaaaaaac gatagatgct
    11101 atagaaaaac tccaaaagaa caaaaaagaa ataattgaat cagaaataga atttttaaaa
    11161 aaagaaaaaa ttgatattgt attttccgat agttcctctc taccatttgc gtttgcaaat
    11221 aagttaaata taccatctta ttttattgga aattttactt gggattttat ttatcagaat
    11281 tatacgaaac aatattcctt tttcaaagat tatgtgaagt taattaggga tgagtattct
    11341 cttgcaacat ttggatttat cctacccttt cattgtccaa tggattatat tccaagaaaa
    11401 aaaaatattg gcattatagg acgaagcccc aaaagaaatc gaaaggagat aagagaatcc
    11461 attcgatttt ctgaacaaaa ccaatacttt ctattttcgt ttggagccta tggcattccg
    11521 gaatcagatc tttcctatat gggtctcaaa gagaatcagt ggatagtggt ttcagggtat
    11581 gaaggtctga aaggaaataa ggttatggat gtaaaagata tctactatcc agacttagtg
    11641 agggcatgtg attatgtttt aactaaacct ggttatggaa ttttatccga aacctatctt
    11701 gcgggaactc caatcatcta taccgaccgt ggagattttg cagagtatga ttatttagtt
    11761 caggcgatgg agacttacca ttattctgct tttctaagtc aaaaagagtt gctctctatg
    11821 aatttagaat ctgcagttca gaagatagaa atacagcgca attctcgaaa aattccatat
    11881 ctttctaagg gagaaatcga aattctagaa caaatttttt aaattgcctc ctttatttca
    11941 cttttcatag ggatatgtat ggtaaatcat ggcgaagtaa gaattttaga cactgagatt
    12001 ctatacttaa attacttatg gagacaagca tgttaaaaaa attagggata cttattctta
    12061 ttacttcatt tttagctgta gctaatgttt ttgctcaaga agaggaaagc gatacaactg
    12121 agccaaaaga agaaatgact gacacaaaag acgctaaaga ggcaaaagaa gcaaagactt
    12181 ctaaaaccga aaaaggcgag aaaaaaggca ataagaaagg tgctaaaaaa ggcaacaaaa
    12241 aaggtggaaa aaaagctggt aaaaaaggtg gaaaaaaagg aaacaaaaaa gctcctaacg
    12301 ctgaaaccaa aacagaaaca cctgatgcag aagaaacaaa ataagagtcg cctttaatag
    12361 attcttacct aaagggatag tttaactatc ccttttttat ttggtagtat tagtctctac
    12421 tatcttaaaa tctttcagtt tatttaaatc ttttagtatt tccttctttt tccctacaac
    12481 cacaatcttc attttatttt gcggaatata gtggttagcc gctttttgaa catcttcagc
    12541 tgtgaccttt cgaatatttt ctaagtattt atcataaaag tcagcaggta aatgataaat
    12601 tgctgtttta attgcaaata gggctagtgt taacggattt tcaagagata aagaaaactg
    12661 cccagtcaaa taatttttaa caagttgtaa ctctttctca tctacctttg ttttttgaat
    12721 tctttctaat tcagaaaaaa tttcctcaat agatttatga gttacattgt ttttagtgct
    12781 cagatctata gaaaaattac ctgctattct atccggctgt aaactagaat agactccata
    12841 ggtgtaacca tttttttctc ttaaattttc ataaagccta aatgttcctc cacctagtat
    12901 tgtattcatt actattacag aaaataaatt tttatcattt ggtttcaaat ctattagttt
    12961 tgatatctta attacggatt gaactgagtc ttctctatcc aataaaaaaa ttttcgttag
    13021 ctcctctttt ggtaaggcgg gaatctttac atcctttact tttcgagaac tccaatttcc
    13081 aagagaactc tccactaacg tctttgcttt atctaaactt atatctccta taatcgcaag
    13141 ataagaatta ccaggtttga aaaatttatt atggtatgct ttgcaatcct caattttaat
    13201 attttgcatt gattcttctg atggaatttc tctatatgga tgattttctg gatagaggag
    13261 tgttttcatc aaattatttg cttccgagtt tggatccaat ttattcgatt taatttcagc
    13321 tatgatttct tcttttattt tttgaaattc atttttatca aaggcaggag attttacaat
    13381 atccgataaa atttctagac tttcctttga atatttttct aaaccactgg catagattaa
    13441 attgttttga attactagat cggttcccat aaaatctaat tcttcatcta actgttgttt
    13501 acttcttttt ccagctcctt ctttcatgag acgagtagtt aattctccca atccatactt
    13561 cgtttttttt attttagtca ggaagtcaaa atacaacaca aaagaaacag taggaagttt
    13621 atgatcttct attaaaaaaa ctgtaagtcc attttctaat ttaaatgttt gataggatga
    13681 tattcgtatc ggtgacaatg gtagagcttt aggaggtata tttaaattat cttcttcact
    13741 aaaaatagag ttagaagaaa aaaaagaaag gaatagagat atgattagga gtaaaatttt
    13801 ggatttcatt attttttgta tttgtttggt aggtagtgta agataatttt tttatctaga
    13861 gtgaaatact tttttgcaac tctttgtatg tcttcttttt taatagataa aaccttttcg
    13921 tattcagtgt taattaagga tgcattccca taaaaagttt catattcagc taaattctct
    13981 gcaatcccag caagatttgc atttgttgca taaatatttt tttgaatttg atttctaact
    14041 ttttctaatt ccctatccga gacaaactct gtttgtaacc tttttagttc ttgttctaga
    14101 ttggcaggaa ctttctcata ggaactttta tcttttgcat aactagtaat agagtagaga
    14161 ccatagtctt ccattgctct ataagttgct tccacattga taatcaaatt attcttagtc
    14221 tttaaattat atactaatct ggagctctcg cctcctgata aaattagatc tacaaagaca
    14281 aaaggataat aatcattagt cccttgattc ggagcatgga aagcataagt taaagatgga
    14341 aattctacat ttgcataaac agttttttga atttcttttt ttaaaggagg ttcaattact
    14401 ttatttcttt ttaacttttt tttagatttc ggaattcgat taaaatattt ttcaactaat
    14461 tctttggtag actttgaatc tatatcgcct gcaataacta agactccatt gtctggagta
    14521 taatattttt tatgaaaatg attgaattct tctaacgttg ctttatcaat atattgattg
    14581 gatcctattg gcaaccattt atatgagtgt tttctaaatg acatttgatg aagaatttct
    14641 attaaatctc cataagggac attatctagt ctttctgatc tttcttcttt taccactttc
    14701 ctttgtgttt ccacgccttc tggattgatt tttaaatgaa gtaatctttc tgactccatc
    14761 cataagatca atggcaattg attggaagga atagatgctt cataataagt ttgatcaaaa
    14821 gtggtatgag cgttagactc cccacctgca tttaaaatta atttatcaaa ttctcctctt
    14881 ttgatatttt tagagccttc aaacatgaga tgctcaaaaa aatgagcaaa tccagtccta
    14941 tttggtgact catcttttga tccaacatga tataaaaaac caacagatac aataggacgt
    15001 gtattatccc tatgtaaaat aacggttaga ccgtttgtaa gtttaaattt ttcaaaattg
    15061 atattaaact tttcagttgc ttgtaagcta ataacaaata ggagtgctat taaatagatt
    15121 tttttcattt tactgtattt gattttaaaa tattatatat atcgggaaaa cttttttcta
    15181 gagaagcaat catcttatca cgtttttctt tatcggttga atttaaaatt tttacaatcg
    15241 aatcaaagcc aggagcattt gcatatgtgt cgtgaattaa ggaagctact ttcttttcta
    15301 aaatctttgc cacttctctt gctatttcaa tatttgcttt ctggcttatt gctatttttg
    15361 caactacatc agtctgaata gattcgggaa gtagagatag aatagaggct gacttttctg
    15421 cacttaaaaa agataaaatc atagatatca tttgtgcttg ttcactttga ataatgccag
    15481 ctatcgtatt tgattcaagt ctctctaaga attcaaatgg attttgattt gaagattggt
    15541 ttggttgaaa attatataca tctggttctt tatctaaatc gattatttcc gaattgtgtt
    15601 tttgttctaa accaacatta ttcacaaaac gaactccaaa ttttccattt tccaatacga
    15661 caatttctcc aggaaaaaat agatcataac caactaaaat ttgaactggt tcgaagattg
    15721 atttatctag ttctataatg gaacccttac tcattgttag taaatctttt atagttagtt
    15781 taagcttccc aagtatcact cgaatatcag ccataggaat tttagtctga gattctatcg
    15841 agcttggcaa tactccctct aaacttttag attttaaatc atttctattg gagatgtctt
    15901 caatccagat accaaatttt tcatttgaaa cgactactgt tgtttttgca aaaacaacat
    15961 tttcaattac aagataaact ggatcattta tatctttatt taattctatt atattacctg
    16021 gtttaatatt gatgagtctg tctaacggta atttcgcttt tccgatttcg atgtctgctt
    16081 caatttccaa gttcattaac tcagtgttag cgagatgcaa ttcatattta ctaggaatta
    16141 catttccagt taaatcatag agtaggaatt tttcgttttc tttttttagt aaaatttcct
    16201 gtaaaggctt atttgaaaaa atcaatgtgc catattttcc aatgaatttt tcaatgttcg
    16261 tatttttatc tgtcttttgt tcaaaagatc tataatcaaa tatttcatct tcaaagcgaa
    16321 cgttaaccgg aaatggtatt gaagatatta gggagtataa tttacctttt ctttcttttt
    16381 ccgcttcgtt taatttatat cttctatcaa tgatttcatt tgaaagggat gtaaaaactt
    16441 taattaaact agcttcaata tcaggctttt ctcctctttc tttaattggt gatagtaaaa
    16501 atattttttc ctcttttgac aaatttttat ttatataatt tagtatttca ttagattcat
    16561 tctcgctcag atatgtttct ctatttttca taaacttttc gagctttgat tctatggaac
    16621 gaatcatcct atgatgactc tttaggtttt tcttaacatt tggatttagt tgtataatgt
    16681 agtttagaat attttttgca ttgtagacag agaagagaaa atgaaaatta aaaacctctc
    16741 cggaagtaac catactgaat tcaattttaa aataattaga gccttcaggc aatataagct
    16801 ggtctgattt ggtaactaac gcttgataca tttcggagga tttaacttcc ccaatcttta
    16861 aagttagagc ttccataaaa gaacaaacta cagaataaag ccaatcgcca atgtttttta
    16921 aatgattgtt tccaaccttc atttcccctt tatctaaagc cttgacaata cgaacagttg
    16981 tatcattaga gagtaggatg gaataaattc cttgtattct gcctagaaaa ttgccataga
    17041 aacattgcat tcctggttta aattgttttc tgattttttt tgcgggaaca atttcaaaac
    17101 caattttttt gcctagttca atatcgccga acttttttga tttaattacc tctgaaagaa
    17161 tagcagataa tatttcctta aataaatttc tttctgaggc agacaattta tcagagagct
    17221 tttttgtgaa atttttaaaa ttctccattt ttaaattttc taatatttca atcgatgctt
    17281 ctcgcatcca gtagtcatta tttaaaacag agaaaggatc agaatgtatt ttgtcatata
    17341 aaggtttatc ttcttttcta gttgttgcct ctaaaagagc ttcaatgtct tttttggata
    17401 atgtttcttc tgacattaga gcacctcagc agagcgaata aagtttttaa aaagagtctt
    17461 gaaataatga ctcgattttg agtcatgcgt taaattgata ttaacctcat gggaaatatc
    17521 ttctgtctct ccattgggtt ggatataaac agtgccagct tgaaactcag agtaggtcct
    17581 gatcatacaa gactctagaa ttttttttct tctctcatat tgtgaattgt ctttattcga
    17641 atcatctata taatctaaaa ttttatttga aaatttatga atgacaggct ctaaacttgt
    17701 atagggtaga cagatgttca ttgaatattc tgttcctttt aaattgagat ccaaacatac
    17761 ttgtaaaaca atttctccag aatatagttt ttgcttgaag aaagtgtttg tttcaatttt
    17821 ttccaagaca ggtttaattt gcaaaacgga ttgaaaagat tcttttagat aggaaactaa
    17881 cgtatgcatt atagtttgaa taatagacaa ttctaaatca gttaattgtc taggtttaat
    17941 gatcggatct cctttaccgc ctgtgagtat atcaattaat gaaaatgtga actctggttc
    18001 gaactcaaag attgtataac catgcaaagg ttttagattg accattccta gaattgaatt
    18061 ttcgggtatt aatttcgaaa actcctcata actcaattga tcaatagaag aaaaatttat
    18121 actaagcgga atttcaaatt tgagagagag agcaggggaa gcaattctag aaaatgtttc
    18181 aaaaatgtat tgaaatgatt taagatggtc ttttgagaat ttatctggtt tcttaaaatc
    18241 gtatgttctt atttttttcg gttcaacagg agcatcatct gacttttcta agctggtagt
    18301 agagattgca gtgagtaagg catcaagttc atcatttgtc agtatttcat ccataaaaat
    18361 tacctaaatg tagaagtatc taaaaaaggg aattttcatg catcaaaaaa aatgagattt
    18421 attaaaaaaa tgaaatttgt ttgtttaaaa taatttaaaa tttaaaaaaa tctactttag
    18481 tcttaagcat ttctgcaact tttgataatt ctagtgattt tgtagctatc acatttgagc
    18541 tttctgtgtt agaatgagaa atttcattaa taaatgtaat tgcttttaca atttcatcga
    18601 aagctacttt ctgttctttg gtaatttgct gaatttcatt ggctctactt tttacaattt
    18661 ctgtttctct ctctactaaa aacttagtcg aaatctgctt ttgcatataa tcgaatattg
    18721 cattcatttt ttcaacaatg gattccactc cagaattgat tgtatttgtt tttttcataa
    18781 tcacatttac agtatcaatt ccttctaaaa tctgtttatt attctttttt ataagtagat
    18841 caattccttt tacactagaa gcagtttggt ctgcaagcct tgaaatttct agagcaacaa
    18901 ctgcaaaccc ttttcccgca actcctgctc tcgcagcttc aatggaagca ttcagtgcta
    18961 gaaggtttat tttttcagaa atatcattaa tgatttggat gatatttttc atatcttgag
    19021 aactttctac gatttgtgtc atacttacat tcatagaacg cagagcttct tctcctgaca
    19081 tggcttcaat agaaattgct tttgtattgc caagagtttt ctggataatt tcattcattt
    19141 cttcaattat accagaaagt tctttaagtt taccagataa cgcatttaaa ttattaaact
    19201 gtatatctgt atgatttgtc acatcgctta cacgttccga gagttgctcg attgaggcag
    19261 taatctcttc aatggttgag gcttgacttt gtgcattcat tgaataggtc ttagtagtat
    19321 cagacatttc acgagaagag gctgcaatta ataatgaact ttcttttaca gactttgtta
    19381 ttgtcataag tctctctgta aataagttaa agttagtcgc taggtgtcca atctcatctt
    19441 cagattttac ggtaatcttt tgattgatct ctccggagga gattgaattt aaagctttta
    19501 ctaaggattg aattggattt gtaatggaat gggttccata ataaaaaata ataaatacaa
    19561 aaagaataga aacaacgcaa acagcaaacg ttccataggc aatgtttctt aaaactccag
    19621 tcatcttaga tcgtggaact tcataaacga ctccccaatc atttatttct aatgggacat
    19681 ggactatatc tgttaatcct ttgtcatttg aatattccat tacagaaact ttagagggat
    19741 cttttaaaat cgacttttct atttctttac tagtaaattt gccaattggc tgattaattc
    19801 cagagacgtc agatgctatc ttaacattgc cctgattgtc gataagccaa actctcgcat
    19861 catattccgg atccgtttct gtaaattctt ttgctacttg attgaatttt atagaaacac
    19921 cagccactcc aattggattt tttatatcac ccattaaaac atttataaaa acgtaagtat
    19981 cattttttga atcagaattg atattaattt gtgttttctt ttctgttttt
//
LOCUS       DAOQOS010000101        19762 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_1363_length_19762_cov_6.262242, whole genome shotgun sequence.
ACCESSION   DAOQOS010000101 DAOQOS010000000
VERSION     DAOQOS010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 19762)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19762)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19762
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1363_length_19762_cov_6.262242"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..242)
                     /locus_tag="PLF66_18830"
     CDS             complement(<1..242)
                     /locus_tag="PLF66_18830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66153.1"
                     /translation="MISIFISKIGIAIMLCLIYYYTKEIFQTKWLLFAVIIWVMFLFG
                     EIGQAIGPNYSWEEAIAGIISETIYVPLSIFVLRII"
     gene            complement(443..2218)
                     /locus_tag="PLF66_18835"
     CDS             complement(443..2218)
                     /locus_tag="PLF66_18835"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HMZ66154.1"
                     /translation="MDRVKKKILLVEDEIITAMLEKEQLENYGYSVTHVMNGEDAVKQ
                     ALDSNLQYDLILMDIDLGLGIDGTQAAEEILKEKDIPVVFLSSHIEPEIVEKTENITS
                     YGYIVKSSSFTVLDASIKMALKLYSAKSELNHSKEKYRLLIDNSITAIASHEMIVDEK
                     GKPIDYIFKSANAAFEIQTGLEIDKIIGKKITEVLPGIENTPFIEIYGKVALNGESIS
                     FEQYSEPLKKYFLIYAYQIKYKEFATVFIDISERILNENYALQSKLLLNAIIESPENL
                     IIMAIDKNYNYLAFNKAHRDSMQFAYNSNIELERSILDYITSEEDKYKAKENYDRAMK
                     GESHSTIEKYGDENKSYYESRYNPILNEKNEVIGITAFASDITERIQREETIQKQLAE
                     KEILLKEVYHRIKNSIISIESLLLLQGESSLNVDVKTALKESVLRIQSIRVLYEKLLL
                     SNHYQNVSIKNYIDSLIDSLIAVFPESKKVFIERQIMDFRITTKKAIPLGIIINELIT
                     NIFKHAFNEKTTGHILIELYKSENQGILTIQDNGVGMEESFPTSKSSGYGLSIVKMLV
                     QQILGTYTVENMNGTKSIIQFEIEF"
     gene            2682..2825
                     /locus_tag="PLF66_18840"
     CDS             2682..2825
                     /locus_tag="PLF66_18840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66155.1"
                     /translation="MDFRLLSDKSKSVNFPFRRDSILAAFSGFSAFRETSAFWKIFFP
                     SKL"
     gene            complement(2998..3762)
                     /locus_tag="PLF66_18845"
     CDS             complement(2998..3762)
                     /locus_tag="PLF66_18845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66156.1"
                     /translation="MCQRYTLEKSIWIKFILFWFIYFVLICNGKNPPLPNLKVDIPPS
                     GEFIPFLEKPESDAKKITQIKNGEFIYSNEAEKVPHLSKLNRENYIYASYKEFQGWVN
                     VDELEYIYKPCPERIENIDLKTIQKGFVYSTERNTYIDSLKIEGGQFGFIAFNNRFHF
                     DNTNKGLFVELKLGICEDNRLVFKFIGHYPKVSFLNYEKRCPEGYTGDVDKYIDKEGR
                     FVLKNVKYFDPEKEDFINIQPTEPIICYDECYISCQ"
     gene            complement(3866..4294)
                     /locus_tag="PLF66_18850"
     CDS             complement(3866..4294)
                     /locus_tag="PLF66_18850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HMZ66157.1"
                     /translation="MALINPHINFNGNAEEAFHFYRSVFGGNFKKIIRLKELSSDEFP
                     VEQIDADKIMHISLPIGKNLLIGNDIPSFLGKVNENENRSKISISAESREEADKLFNG
                     LSVGGAVEVPMNDGPWGSYFGMFRDKFGIEWIVEFDPKNN"
     gene            4866..5219
                     /locus_tag="PLF66_18855"
     CDS             4866..5219
                     /locus_tag="PLF66_18855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66158.1"
                     /translation="MNMKLYLSILFLILLFYSCGASNTCRKNCDKDSLACYLIFSDKS
                     IPFGTQLLGLLFCDLNKDNCLSKCSSGSGGSSSGSSRSSSSGSRSSGGGSSGGGGGGS
                     SGGSGGSSHEINSPL"
     gene            complement(5452..6804)
                     /locus_tag="PLF66_18860"
     CDS             complement(5452..6804)
                     /locus_tag="PLF66_18860"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HMZ66159.1"
                     /translation="MKRVQLIFLILILALCHCKKKDSNLSKEDISKLPKIGEFYIYKS
                     NIPIYESPNRAKQIDKVNIGEKLFILETKIPDTVQGFWYKVLHGDTLGFIPLSEETNK
                     HLVAFLYQQERGRIIASSLRIRETPDLKGKVLGSVPKHTIIDILWQGLVYEKIDDKYD
                     TWMKIQTDSGLLGYSYAGYISKNLDPDLDEVEAESIYGYVEIKESPTYLVRPGGREVK
                     DSDPAPCGYNAIGSLPQQGVIHKTLFKYVDKGITYYKIESAEDSHGCYESYRGWISEN
                     QVTFISDIYKYTFENYGSKFDKQFLDVINENVNHELNVKTLVIEPFNFKVKEPGYTFY
                     KVHGGHVYYLYNNQYHYAGYGGWGDLVDIDSDGTNEIISGGDCACMCSEARVVIWNGS
                     KLDTIFQEYPTASLRWETGKNFLTAIRSSSYDNSEPGEKTYYEIKNNQLVELKKKPKT
                     "
     gene            complement(6898..7539)
                     /locus_tag="PLF66_18865"
     CDS             complement(6898..7539)
                     /locus_tag="PLF66_18865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002728580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RibD family protein"
                     /protein_id="HMZ66160.1"
                     /translation="MKLQLSINMAMTLDGKVARPDGKWYGLSSRNDKRKMDVIRSEAD
                     ALIVGKNSILNDNPVIKLRYVEGKNPLPIILIRKGSLPKTKHIFQQADRKPLILCTPQ
                     NEKEIRQDLQEYAEILSLSSNEIEPKEVLSILSEKGFQKVLLEGGPTLNYAFQKEGLI
                     DVINLTIVPFLIGQKSLPSIVTGDFPFPNFDQEKWKLVSFEKLENEIFLRYEK"
     gene            complement(7552..7788)
                     /locus_tag="PLF66_18870"
     CDS             complement(7552..7788)
                     /locus_tag="PLF66_18870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010572859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HMZ66161.1"
                     /translation="MSEEVDLSSLYSLMRPKKVCLCKSVTEAQLVAAIESGCDTMEKL
                     IDVTHASTNCGTCSGSVRAIFNREMDKKKTQAKQ"
     gene            7980..9323
                     /locus_tag="PLF66_18875"
     CDS             7980..9323
                     /locus_tag="PLF66_18875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017856346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HMZ66162.1"
                     /translation="MEVKERVSFKELFGWCMFDFANSSYTTVIISVIYCDVFSRLVVP
                     AGSNPNNPNQEGNLLWGIALFISYMLVVVTGPILGAITDFSARKKLFLFGSYFGCIVA
                     TSLLWFVAEPGFSYIWMAFFLIIISNFFFASGENIASSFLPFLGPKDDLGKISGYAWG
                     IGYFGGVASVALVSSLGEVTTENYQALRLVGPYTAAFFLIAGTPTFTFLKEYGAALPK
                     PEGRSYVRIGFETVVRTLRDISKFKDMQIYLISLFFSMAALGVVISFAFIYGAQEIHI
                     ENVHRQAMFILIQISAAAGAVAFGFLQDRMGAKRTFNITLVIWIICLMAIHQVVNLTN
                     LVNSILSTKITVQWMFVIITSLAGMGLGSTQSSSRAIVGMFAPESKTGEFFGLWGLSG
                     KLAAAVGIFSVSLLQMMFSLRNSFLAIAFFFIISLLINFLVDEERGIATARNYKG"
     gene            9342..9557
                     /locus_tag="PLF66_18880"
     CDS             9342..9557
                     /locus_tag="PLF66_18880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020772139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66163.1"
                     /translation="MLLTEDEIELYQNQKNLALSTIDDLTDLKIQLLESGVQVPYFIN
                     NSIEYLKKKYLIQDHTIGQVLRTHAGG"
     gene            complement(9554..10567)
                     /locus_tag="PLF66_18885"
     CDS             complement(9554..10567)
                     /locus_tag="PLF66_18885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66164.1"
                     /translation="MKTLIVLLVVTANILLAEPTTLRQEEKDELKYIDSIRMHGFADR
                     IIDRLHKSIALNITRIKELADKNYLKNGYRYLQHEPIDESIINKNQLFFTDKENKQYL
                     KLDLSQGVTYEDYPNRHIFDTKAFVYLNPDGKSLSKVIFQFSRVNSTGTNYIKEVRRI
                     INPTPNSPEPLKREGELVNDVKKLQEENILDDSVAPDTNSDIIVEYYSGHDKDLPPID
                     PPKLASESTKTSPEANANPNAPKPEANLQTRENSTKPRDPSYKAKDLPWVNDSPDMTN
                     LASMSNRLNDPNELLPFASQKHIYDTYRIVLKVLNREIERKVKNIELNQRIIIKKMTE
                     INF"
     gene            complement(10610..11203)
                     /locus_tag="PLF66_18890"
     CDS             complement(10610..11203)
                     /locus_tag="PLF66_18890"
                     /inference="COORDINATES: protein motif:HMM:NF023238.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3332 family protein"
                     /protein_id="HMZ66165.1"
                     /translation="MSLRIRTITIALLISILPLTMNCFGKFALTRKAYQFNETLGGEG
                     WFWKFTRTFIAYIYFLFPVYVVLFSDALVFNLIEFWTDNNLIGYNEYDKNGIYVKYYQ
                     KEKESLTLTYLNFGKRLNLEVSDGVVSEKFVVYRNEPGVLYRENKGKIQKVDVQTKDI
                     GSKRIIKILNEGKLETSIVKEKEDFQKLEKKYLGEFY"
     gene            11440..11970
                     /locus_tag="PLF66_18895"
     CDS             11440..11970
                     /locus_tag="PLF66_18895"
                     /inference="COORDINATES: protein motif:HMM:NF019235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1564 family protein"
                     /protein_id="HMZ66166.1"
                     /translation="MKNKPNHSKSFSTQKISFPRDHFTFSEFSSTLLIPEKYMSLFQK
                     KIVLHKGIRSYVAYLLKKYKLYIANGLVPGYSNVTTKYQEKGQNLHKIAFRPNPEDWA
                     ELKLYRVSFGMSISAFFVYLVIADSVELAETLSDYLTRVGISVVPNFDLIAKVYLWQK
                     RSFYTTIFQYRESYSH"
     gene            complement(12163..12765)
                     /locus_tag="PLF66_18900"
     CDS             complement(12163..12765)
                     /locus_tag="PLF66_18900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016543751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3332 family protein"
                     /protein_id="HMZ66167.1"
                     /translation="MKKFFQKFILLLLMVGITLTSFANCFGKFSLTRKVYAFNEGIGG
                     GDWAGKIIRTLLMYVYFFFFIGGLVFFIDIVILNLIEFWTDSNPLGLNEYNKEGKYVK
                     SFSKDGETLKLTYINFGQKLYIDMSNGEKSEQLVVLRSEKGKFFKEKDDKLEEVSIDS
                     INVGSKMILRMATNGKLESSKVVETKDFKQLESKYAGEIY"
     gene            12990..13295
                     /locus_tag="PLF66_18905"
     CDS             12990..13295
                     /locus_tag="PLF66_18905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66168.1"
                     /translation="MNFKKIDIKDFLLGFLTCLCLFLLTSIAGCGRTLSRLGLGDEKL
                     NLPKDFKTMVSVSFHKESNGDTIKDMTYETLDGKFRSVEYRDKLWHMEGGITWEKDN"
     gene            13417..14874
                     /locus_tag="PLF66_18910"
     CDS             13417..14874
                     /locus_tag="PLF66_18910"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004468117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HMZ66169.1"
                     /translation="MLFTSIEFFIFFLFVFIFQWYIHPLFAKANDEQQSYSLHIFLLL
                     ASYYFYMSWDYRFGALILISTLIDFFLAIQIENTEVKSKRKFYLVISLFLNLICILGF
                     FKYYNFFARSINSTAGNFGYSSFFPILNIILPVGISFFTFQSLSYTIDVYRKIIPVER
                     SLVKFALYVSFFPQLVAGPIVTAKTFLPQLFRQVKLEEVNFKKAIRYFLLGYFKKVII
                     SDNISPIVDLIYKNPENYGTEALWLATSLFAIQLYGDFSGYTDMAYGSALLLGYELPE
                     NFFMPFKAVSVSDFWRRWHISLSTWLRDYIYISLGGSRNGYFRHKFNLFMTMFLGGIW
                     HGANWTFFIWGTTMGLFLAIESMFNDWKEKNAKDFFHRFRIPINIIGGIYALGAVSIT
                     CALFRGESLEKSLLMLHNMVHFVPGSPRPYMIKTALIMFVIVFLGHSIGHYLYERGKV
                     FKIPYWLEVSFYPFIILGIAYFTNDNEAPFVYFQF"
     gene            complement(14876..15571)
                     /locus_tag="PLF66_18915"
     CDS             complement(14876..15571)
                     /locus_tag="PLF66_18915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015678792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66170.1"
                     /translation="MQFFLIIILILFNCKPADPNYRTKPLLNTEYTGFISRNFFQAVV
                     EVPITKEELTILEERKSCLKDAIRKRDTIVLPLLKKIAMEDKWNDKERSLEKDRLSDS
                     EKYKEKSEELKRNENAKSSEEASIFDKKKTDPVQSKNPLMNRGEFSWFLDSMFLYKED
                     YSNPEKCSFVFRNIQDKLYEKVEKTKLSIIGEDRVNPFNPATTPTNPPPTGATTPANT
                     TSPLNTGLPQSVR"
     gene            16057..17421
                     /locus_tag="PLF66_18920"
     CDS             16057..17421
                     /locus_tag="PLF66_18920"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HMZ66171.1"
                     /translation="MRVNFIVIFLILFSYSDLFSQKKTETVSLKGNEMGAKRWAIIVG
                     INEYSDKQISSLQKARNDAKLIAQILEEQGQFEEIHLMTDDLSSKNPLYPTKSNIESK
                     IDHVLDFSSPVDTILFFFSGHGISDSNGNGYLLTVDSSLDKSLLTSLTVNTIIKKIKD
                     RNVSKSILFLDACRDVTNSKSFLKEGLKSEKSSSEDFPILFYSTTTGYFSYEDSKSSF
                     GVFTKYLAHGMEGKADTNQDGIVSFGELVDFIQTGVVGWSDQNEKEQKPYVAYNREKF
                     GKIPITASVDKLTSLVDENSFPKSNSKLGAVIRSFVTPGWGQWYMGASEKGLSYFSIF
                     LVLLGNAAYQYQPYQKAKANYESTILIPATPGGGDTLGINYLLFQPKLDAVNHAKYNY
                     NLSIGAVGVFWAWNLLDLFLFKGNNFFWAVNFRLTPVDNSTIYAHSSIQLDQKKEIVF
                     TLRF"
     gene            17422..>19762
                     /locus_tag="PLF66_18925"
     CDS             17422..>19762
                     /locus_tag="PLF66_18925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66172.1"
                     /translation="MKNKFLNALVFIYLIFNFSSCVGAVKRSILDPNSFLGFFLSLDP
                     FKLNPPPKSKITLLNTISSIQEGESTTMGILLQNNPYKQITVTCSTDYPSLLINGARS
                     TTFVFDSSNFSTQQSLNLTTVTDNDNTSDNVTLSCSATGLDTVTASITVSDNAGIILS
                     GTVPTLLEGDTVTIGVSLSRAPTTNVTVNVSLNNSAVTINSATTLSLTFTPSNYNTSQ
                     SITILSLIDGNSTNDPTTLTFQMTGIADVTYNLTIIDDNVIQLTGPPTSITEGTSTLM
                     GIRYFKSLLTNNSITITSSNPAAITVNGGASATFNYTPGNATTDQNVTLAAVLDANLV
                     SETVSLTLTTTGMDPLVIVVTTVDIDVQNMVITGAIPTLTENMNGSILVHLTNLPPTN
                     VTVNITTSNASSLTVSPSTLTFTPGNYNIDQAVTVNAIDDANETAEYLTVIFTSTSAP
                     MQIYNITTIDDDTRILLSGASSVNEGGSSVVTVSLSGDPGIPRTINLTSSNLLALTLG
                     TASLSFSSGNYSQSISINGVQDANIVSEAVTISATSAGLISGSTSINTVDDDTMNILL
                     TAGSNLVSEGSTSTLNLRLTQEPSPSLTVTLNSSIAGSVSLSPASFTFDNVCPGPQCW
                     STNQVVTLTGVEDVNQTSETSVITASAPAVTNVTQNITTIENDTTPVFTGATTVSETG
                     TSVITVSLSGDPGATRTVSLVSSNLLAMTVSPSTLTFNSANWNIPQTILVAGVPDNNI
                     ISESVTITGSGVGLITSTTTVNTVDTTTMAITLSGNPTSI"
BASE COUNT         6216 a         3439 c         3101 g         7006 t
ORIGIN      
        1 ataattattc ttaaaacgaa aatggataaa ggaacatata tcgtttcaga tataattcct
       61 gcaattgcct cttcccatga ataatttggt ccaatggctt gaccaatttc accgaaaaga
      121 aacattaccc aaattattac cgcaaagagt aaccatttag tttgaaatat ttctttcgta
      181 taataatata tgagacaaag catgattgcg attccaattt ttgatataaa aatgcttatc
      241 aaaaaaaact tcgggtgaaa ttccttctgg tggttttaaa ttaaataaag cagtaaaaat
      301 tgaataaatg ataaagggaa taataaaatg atcgcagttg ttgaaactat tcctaaaaat
      361 attttactca taaattccct tcaaatttta tttttaataa aagtatatcg cttaacgacc
      421 atactgattc gaaggtaagt gcttagaatt caatttcaaa ctgtataata ctctttgttc
      481 cattcatatt ctcaactgta taagtcccta gaatctgttg aactagcatt ttaacaatag
      541 atagaccata tcctgatgat ttgctagttg gaaaactttc ctccatacca acaccattat
      601 cctgaatcgt taatattcct tgattctctg atttatatag ttcaattaaa atatgacctg
      661 tcgtcttttc attgaaggca tgtttaaata tattcgttat caattcattt ataataatcc
      721 ccagaggaat cgcttttttt gtagtgatcc taaaatccat tatttgcctt tctataaaaa
      781 cctttttgct ttctggaaaa actgctataa gcgagtcaat cagactatcg atatagtttt
      841 ttatggaaac attttgataa tgattgctca acaataattt ttcatataga acacggatac
      901 tttgaattcg taatacggat tctttgagag ccgttttaac atcgacattt aaacttgatt
      961 ctccttgtaa taaaagaaga ctctcgattg agataatact attttttatg cgatgataaa
     1021 cttcttttaa tagaatttcc ttttccgcaa gctgtttctg aattgtctct tctctttgta
     1081 tgcgctctgt aatatcggag gcaaatgcag ttatacctat aacttcattc ttctcattta
     1141 atattggatt ataacgactt tcataatatg atttattttc atcaccgtat ttttctatcg
     1201 ttgaatgaga ctcgcccttc attgccctat cataattctc tttcgcttta tatttatctt
     1261 cctcggatgt gatataatcc aatatactcc tttcaagttc tatatttgaa ttgtatgcga
     1321 attgcattga atctctatgt gctttgttaa aggctagata attataattc ttatcaattg
     1381 ccattatgat taaattttcc ggactttcga tgattgcatt cagtaataat ttactttgta
     1441 atgcatagtt ttcatttaag atcctctcgc taatatcaat gaatacagtt gcgaattctt
     1501 tgtatttgat ttggtaggca tagatcaaaa aatatttttt taatggctct gaatattgct
     1561 caaaactgat tgattcacca ttcaaagcaa cttttccgta tatttctata aatggagtgt
     1621 tttcaattcc aggtaaaact tctgtaattt ttttcccaat gattttatca atttccaatc
     1681 cagtttggat ttcaaaagca gcgttagcag atttgaatat ataatcaatt ggctttcctt
     1741 tctcatctac aatcatctca tgcgatgcaa tcgctgtaat agaattatca atcagaagtc
     1801 tatacttttc cttgctgtga tttaattcac tttttgcact ataaagcttt aaagccatct
     1861 tgatcgaagc atccagaact gtaaaactag atgacttcac tatatagcca taagatgtta
     1921 tattctctgt cttctcaaca atttcgggct caatgtgtga tgataaaaat acaaccggta
     1981 tatctttttc ttttaatatt tcttcggcgg cttgcgttcc atcaatacct aagccaagat
     2041 caatatccat tagtattaaa tcatattgta aattactgtc taatgcttgc tttactgcat
     2101 cttcaccatt cataacgtga gttactgaat acccataatt ctctaattgc tctttttcaa
     2161 gcattgcagt tataatttca tcttctacga gaaggatttt ctttttcact ctatccatat
     2221 tgttttatcc tccttgaatt tattgtatat cgaatcggtg caatatcact tatagcattt
     2281 cagtagaaaa tattggataa aataaaaaaa cggaaaggct ttacaataat atatttcaac
     2341 tattgtatat tttttcttat caaagtaaat aagcaaacga gaatccttct tatgataaga
     2401 attcagaaaa aaaacactcc ttgctttata aaaacagaac tgaaagtata ccaaataatt
     2461 agtaaaccag actaaaaatt gagttttaga cttagaatct tttaatagaa gaaaggagat
     2521 ctatcgctta taaaactaag aataatgttc ccatctactg aattaaaggt gattggagca
     2581 aaatttttcg gattctcata gagtcgttgt gcgatatcct taggcatcat tgtctttgcc
     2641 cctgaaggaa taggaagata ttttttaaaa ctttgccttc tatggatttt agattactct
     2701 ctgacaaatc taagtctgtt aattttcctt ttaggagaga ttcaatctta gcagccttct
     2761 caggattttc tgcttttaga gaaacgagtg cattctggaa aatatttttt ccatctaaac
     2821 tataaaattt tgaaataact ttatttttaa ttttgattta aatagattag caatttgaaa
     2881 actactctgt atttcaatca ttcctcattc tagaaataaa ttttcatttt ttatttcttt
     2941 gtccgaggta ttgatccaac atatttgtat tcacaaatat ttgtgtccat ttttttatta
     3001 ctgacaagaa atataacatt catcataaca aataataggc tctgtcggtt gaatgtttat
     3061 aaagtcttct ttttcgggat caaagtattt tacgtttttc aaaacaaagc gtccttcttt
     3121 atcaatgtat ttatctacat cacctgtgta gccttcagga catctctttt cataatttaa
     3181 aaaacttact ttcggataat gtcctataaa tttaaagaca agtctattat cttcgcaaat
     3241 accgagtttt agttctacaa ataatccctt gtttgtatta tcaaaatgga accgattatt
     3301 aaaagctata aaaccaaact gtccaccttc tatctttaaa gaatcaatat aggtatttct
     3361 ttctgtagaa taaacgaatc ccttttgaat tgtttttaaa tcaatatttt caatcctttc
     3421 agggcaaggt ttataaatat actcaagctc atctacattt acccaaccct gaaattcttt
     3481 gtagcttgca tagatataat tttctctatt gagtttactt agatgaggaa ctttttctgc
     3541 ttcattcgaa tagatgaatt caccattttt tatttgagtt attttttttg catcagactc
     3601 tggcttctct aagaagggaa taaactctcc agaaggagga atatctactt ttaaatttgg
     3661 gaggggagga ttttttccat tacaaattaa aacaaagtag atgaaccaaa ataaaataaa
     3721 tttaatccag attgattttt ctaaagtata tctctggcac ataacgttat tttttataac
     3781 tagactaaat ggatcgcaaa caatttatct ttcaattctt tcgcagttaa cttgtgcata
     3841 aacgttaacc gcttccttat actctctaat tattttttgg gtcaaattct acaatccatt
     3901 cgataccaaa tttatctcga aacattccaa aataagagcc ccaaggacca tcattcatgg
     3961 gaacttccac tgcgccacca acagaaagtc cattgaataa tttgtctgct tcttccctac
     4021 tctcagcgct gatagatatt ttcgatctat tttcattttc attgaccttt cctagaaagg
     4081 aaggaatatc attgcctatt aataaatttt tcccaatcgg tagagaaata tgcatgatct
     4141 tatctgcatc gatttgctcg actggaaatt cgtcacttga gagttctttt aaacgaatga
     4201 tttttttaaa atttcctccg aatacagatc tgtaaaaatg aaatgcttct tctgcatttc
     4261 cattgaagtt aatatgtggg ttaataagag ccatagattt tttagatatt cctttgttat
     4321 aatttttata tgtttcctca tcactctttt gcactagctt tgattagcca tttgcccatg
     4381 ttcttaagaa ggatttttga cctcaatcca aatttattca cacaacactt acagattaat
     4441 tccttacctt ccacattaaa tcaactctag atctcttata atttttttaa atgaattaag
     4501 caagtgtatc tgaatttaag atgttctttc gatatgagaa ggattctttc tacaaagctc
     4561 tagaaatctt acccgtgata caatggagat ttttatagaa aaataagtct cgtagtttta
     4621 atttacaaaa actattctag atttaacttt ataaacgata gacgctttat aaatttaata
     4681 taaatatgga aattatctat atttatcgct gaaagacttt ttatttttag attcgcactc
     4741 atactaattt ttttatagag aatgaaattc atttttttat tggatcaaag attgcagaaa
     4801 tagaataata ttaaatcctt ttcccagaaa gtttttcttg catttttgag aatagaaatg
     4861 atagtatgaa tatgaagtta tacctatcga ttctcttttt gattttactt ttttatagtt
     4921 gtggagcaag taatacatgc agaaaaaatt gtgataaaga ttcattggca tgttatctta
     4981 ttttttcaga taagagtatt ccatttggca ctcagctttt gggtttacta ttctgcgatt
     5041 tgaacaaaga taattgtttg agtaaatgtt caagcggaag cggtggttca tcctcaggct
     5101 ccagcagatc gagtagttca ggaagtagga gttcgggggg aggaagttct ggtggcggtg
     5161 gtggaggtag ctcgggagga agtggaggaa gcagtcacga aatcaattct ccattataaa
     5221 ttttctgaat ccgcgtagag attgactatc acagacctgc actagttcta atggataaag
     5281 acgatgggta tcctatatag ttagattttt tgatttgaaa tgatttgctg attgagctaa
     5341 gcaaaagatt caaagtttgt tctttgaatc gatttcatca caatcattga tccagaatag
     5401 aaacccatcg tttttatcgg tgccgttggt tgtaatatta ggttttagtc ttcatgtctt
     5461 tggttttttc ttaagttcta ctaactgatt gtttttaatt tcataataag ttttttcacc
     5521 aggttcagaa ttatcataac tagaagagcg aatggcagtt agaaaattct ttcctgtttc
     5581 ccaacgaaga cttgctgtag gatattcctg aaagattgta tctagctttg agccattcca
     5641 gataacgact cgcgcttcac tacacataca tgcacaatct ccaccagaaa taatttcatt
     5701 tgtcccatcc gaatcgatat cgactaaatc tccccatcct ccataacctg cataatgata
     5761 ttggttattg tatagatagt aaacatgccc accatggact ttataaaatg tataacctgg
     5821 ctctttgact ttaaaattaa aaggttcaat gactaacgtt ttcacattca actcatggtt
     5881 tacattttca ttgatcacat ctaaaaactg cttatcaaac ttagatccgt aattttcaaa
     5941 agtatattta tatatatcag aaataaaggt cacttggttt tcagaaatcc aacctctata
     6001 actttcataa catccatgtg agtcctctgc actttcaatt ttataataag taattccttt
     6061 atcaacatac ttaaacaatg tcttatgaat tacaccttgc tgtggcaaag aaccaattgc
     6121 attataacca caaggagcag gatcgctgtc tttcacttct cttccgccag gtcgaactaa
     6181 ataggttgga gattctttaa tttcaacgta tccataaata gattccgctt ctacttcatc
     6241 taagtctgga tctaaatttt tagaaatata tcctgcataa ctataaccta ggagtcctga
     6301 atcagtttga atcttcatcc aagtatcata cttatcatct attttttcat acacaagacc
     6361 ctgccataaa atatcaatga tggtatgctt gggaacagag cctaagactt ttccttttag
     6421 atcgggtgtt tctcggattc gaagagagct tgcgatgatt ctacctctct cctgttgata
     6481 gaggaaggct actaaatgtt tatttgtttc ctccgaaagc ggaataaatc caagtgtatc
     6541 tccatggaga actttatacc agaaaccctg aacggtatca gggattttgg tttcgagaat
     6601 aaataatttt tcccctatgt ttactttatc aatttgttta gcgcgattcg gagattcata
     6661 aatggggatg tttgatttat aaatgtagaa ctctccaatc tttggaagtt tagagatatc
     6721 ttctttagaa agattgctat cttttttttt acaatggcaa agagcaagga tcagaattaa
     6781 aaaaataagt tgaactcgtt tcataaacac aaagccacca tattacaaat aaaatgtcaa
     6841 gttgtttaga agaatctact tgctatttag aaaatatcac aatgaatttt atctaaccta
     6901 tttctcataa cgtaaaaaga tttcattctc taatttttca aaagaaacta gtttccattt
     6961 ttcttgatcg aaattaggaa aaggaaaatc tccagttaca atggaaggaa gacttttttg
     7021 tccaatgagg aaaggaacta tggttaggtt gattacatca atgagtcctt ctttttgaaa
     7081 ggcataattc aaagttggtc cgccttcgag taaaactttt tgaaatccct tttctgataa
     7141 aatagataag acttcttttg gctcaatctc attagaagag agagatagaa tctctgcata
     7201 ctcttgtaaa tcctgtcgaa tttctttttc gttttgggga gtgcaaagga taagtggttt
     7261 tcgatctgct tgctgaaata tatgtttggt tttcggaaga ctccctttgc gaatcaaaat
     7321 aatgggaaga ggattttttc cttctacata tctaagtttg atcacaggat tgtcatttag
     7381 aatcgaattt tttccgacga tcaaagcatc tgcttcactg cggattacat ccattttccg
     7441 cttatcattt cgagaggaaa gcccatacca ttttccatca ggtctggcaa cttttccatc
     7501 taaggtcatc gccatgttaa ttgaaagttg taacttcata ggaataatct tttattgctt
     7561 ggcttgtgtt ttctttttat ccatctcacg attaaagatt gcccgcactg agccagaaca
     7621 agttccacaa tttgttgagg catgggtaac atcgattagc ttctccattg tatcacaacc
     7681 actttcaatt gccgcgacca actgtgcctc tgtgactgat ttgcaaaggc atactttctt
     7741 tggtctcata agtgaataca gactggatag gtctacttct tctgacatat ataactccta
     7801 ttactattag attgatttat atgggaaatc actgtaaata tattctgttt ctacctaatc
     7861 gtctctaaaa gattttctac ttcgaaaaag aaacatcagg aattgtaaat atttcttgcc
     7921 atttggcaag taaatttatg cgattactag aaaggcatta aaatttaaat gaggtcaaaa
     7981 tggaagtaaa agaaagagtt tccttcaagg aactttttgg atggtgtatg tttgattttg
     8041 caaattcatc ttacactaca gtcattataa gtgtaattta ttgcgatgta tttagtcgtc
     8101 ttgtggttcc agctggatca aatcctaaca atccaaatca agaagggaat ttactttggg
     8161 gtattgcctt atttatctct tatatgttag ttgttgtgac tggtccgatt ttaggagcca
     8221 tcacagattt ttctgcacga aaaaaacttt ttctatttgg tagttatttt ggatgtatcg
     8281 ttgcaacatc acttctatgg tttgttgcag agcctggttt tagttatatt tggatggcat
     8341 tttttctaat tatcatttcc aatttctttt ttgcatccgg tgaaaatatc gcttcaagct
     8401 ttcttccttt tctcggtcca aaagatgatt taggaaaaat ttctggctac gcttggggaa
     8461 ttggttactt cggtggagtg gctagtgttg cgttagtctc ctcattggga gaagttacaa
     8521 cagaaaatta tcaagcattg cgtttagttg gtccttatac agctgctttc tttttaattg
     8581 cgggaactcc tacttttaca tttctaaaag aatacggtgc agctcttcca aaaccagagg
     8641 gtagatctta tgtgcgaatt ggttttgaaa ctgtagtgag aactctaaga gatatttcga
     8701 aattcaaaga tatgcaaatc tatttaatct ctttgttttt ctcaatggct gcacttggag
     8761 ttgtaatcag tttcgcattt atctatggag cacaagaaat tcatattgaa aatgtgcaca
     8821 gacaagccat gtttattttg attcaaatct cagctgctgc tggtgctgtg gcatttgggt
     8881 ttttacaaga tagaatggga gcaaagagaa cctttaacat tacacttgtg atttggatta
     8941 tttgtctaat ggcaattcat caagttgtaa atttaaccaa tttagtaaat tctattttat
     9001 caacaaagat cactgtccaa tggatgtttg tgattatcac ttctcttgca ggaatgggac
     9061 ttggttctac acaaagttct agtcgtgcaa tcgttggaat gttcgcacca gaatcaaaaa
     9121 caggtgaatt ttttggtctt tggggtttat ctggtaagct ggcagcggct gtgggaattt
     9181 tttctgtttc tcttttacag atgatgttca gtttaagaaa ttcgtttctt gcaatcgcat
     9241 ttttctttat aatctccctt ttgatcaatt ttttagttga tgaagagaga ggaattgcta
     9301 cagctagaaa ctacaaagga taatcttgat tcggaggaac tatgctactc acagaagatg
     9361 aaattgaatt gtatcaaaac caaaaaaatc tggctctctc taccattgat gatttgacag
     9421 atttaaagat tcaactttta gaatctggag tccaagttcc ttattttata aataactcga
     9481 ttgaatatct aaagaaaaaa tacctgatcc aagatcatac gattggtcag gtacttcgaa
     9541 ctcatgcagg tggttagaaa ttaatttcgg tcatcttttt gatgatgatt ctttgattta
     9601 gttctatatt tttaaccttt ctttctattt ctctatttaa aaccttcaaa acaatacgat
     9661 aggtgtcata gatatgcttt tgactcgcaa atggcaataa ttcatttgga tcgtttagcc
     9721 tgttactcat agaagctaga tttgtcatat ctggggaatc attcacccaa ggtaaatcct
     9781 ttgctttata agatggatct ctaggtttag tagaattttc tctcgtctgt agatttgctt
     9841 ctggctttgg tgcatttgga tttgcattgg cctctggaga agtttttgta gactcactcg
     9901 ctaacttagg cgggtcgata ggcggtagat ccttgtcatg cccagagtaa tattcaacaa
     9961 tgatatcact atttgtgtct ggtgcgacgc tatcatctaa aatgttttct tcttgtaatt
    10021 tctttacatc atttacaagc tcaccttctc ttttgagcgg ttctggacta tttggagttg
    10081 ggttaataat ccgacgcact tctttgatat aatttgttcc agtggaattc acacgactga
    10141 attgaaaaat tactttggat aaggatttgc catctggatt taaatagaca aatgcttttg
    10201 tatcaaagat atgtcgattc ggataatctt cataggtcac accttgactt aaatccaact
    10261 ttagatactg tttgttctct ttgtctgtaa agaaaagttg gtttttattt ataatggact
    10321 catcgattgg ttcatgctgc aaatatctgt atccattttt taaataattt ttgtctgcta
    10381 attccttaat acgagtaata ttgagcgcaa tagatttatg taaacgatct atgattcgat
    10441 cagcaaaacc atgcatacga attgaatcta tatattttaa ttcatctttt tcttcttgtc
    10501 tgagtgttgt tggttctgct aaaagaatat tagcggttac taccaataaa acgatgagtg
    10561 tcttcatttt tttctccttc tactattatc gtttttaaaa aaactaaaat taatagaact
    10621 cacctagata tttcttctct agcttttgaa agtcttcttt ctctttcaca atggatgttt
    10681 ctaacttgcc ctcatttaaa atttttataa ttctcttcga gcctatatcc ttagtttgca
    10741 catctacttt ctgtattttt cccttattct ccctgtataa tactcccggc tcatttctat
    10801 aaaccacaaa cttctcagat acaactccgt cagatacttc caaatttaat ctcttaccaa
    10861 aatttaaata ggtaagtgta agagattcct tctctttctg atagtatttt acataaattc
    10921 cattcttgtc atactcattg tatccaatca aattattatc cgtccaaaac tcgattagat
    10981 taaaaaccaa ggcatcagaa aataatacga catagaccgg aaataaaaag tatatataag
    11041 ctataaaagt cctcgtaaat ttccaaaacc atccctctcc tcccaatgtt tcattgaact
    11101 gatatgcctt tcttgttaat gcaaactttc caaaacaatt catcgtaagt ggtaatatac
    11161 taattaataa tgcaattgtg attgttctaa ttcttaatga catagtttac tcctagtttt
    11221 taaataaccg attttttccg tttgatgatc tctccctcac caacagttat tttttgttta
    11281 ttgtaacact cgtaataagg ctagactttt ttccaataat tgcccttttt tttgttggta
    11341 ggaactccaa tttgcttttt ttattttttt gcgcacacat tcccagtttc tataccctat
    11401 tctttatagc ataactctta ttcataacga ggtctatcta tgaaaaataa accaaatcac
    11461 tctaaatcct tctcaactca aaaaatctcc tttcctagag atcatttcac tttttctgaa
    11521 ttcagctcaa cccttctcat tcctgaaaaa tatatgtccc tttttcagaa gaaaattgtc
    11581 ttgcacaagg gaattcgtag ctatgtcgcc tatcttctta agaagtataa gctttatatt
    11641 gcaaatggtc ttgtgcctgg ttattcaaac gtaacaacta aataccaaga aaaaggtcaa
    11701 aacttacata agatagcctt tcgccctaat ccagaagatt gggcagaact taaactctat
    11761 cgtgtaagtt ttggtatgtc tatctctgct tttttcgttt acctagtaat agcagattcc
    11821 gtagaacttg cagagaccct ctccgactac ctaacgcgag tcggaatttc agtggtccct
    11881 aattttgact tgatcgcaaa agtttatctt tggcaaaaaa ggtctttcta taccacgatt
    11941 tttcaatatc gagaaagcta ttctcattaa tataaatttt ttttgaacaa aacaaaacaa
    12001 gattaacaaa ggccaacttg gaggcagagc ggagctatat aaataggata atcaattact
    12061 aggataagat tagcagagag gaagaagtca aaatatttac aagatcaaaa gaaattgtaa
    12121 atattttgac attaagaata agcttagaag aacaaaaaga tattaataaa tttctccagc
    12181 atatttgctt tcgagttgtt taaaatcttt tgtttctaca actttagaag attctaattt
    12241 accgttagtt gccattctta agatcatttt agaaccaacg ttgatagaat cgatagacac
    12301 ttcttctaat ttatcatctt tttctttgaa gaatttgcct ttttcagatc tgagaacaac
    12361 aagttgttca gatttttcac cgttagacat atctatatat aatttttgac caaagttgat
    12421 gtaagtgagt tttaaggttt ctccatcttt tgagaaagac ttaacatact taccttcttt
    12481 attgtattca ttgagaccaa gaggattaga gtctgtccag aattcaatca ggtttagaat
    12541 aactatatca atgaaaaata ctagtccacc gataaaaaag aaaaaataaa catacattaa
    12601 taatgttctg attatttttc ctgcccagtc tcctccaccg attccctcgt taaaggcata
    12661 cactttacga gttaaagaaa atttaccaaa acaattggca aaggaagtta aagttatacc
    12721 taccattaaa agtagcaaaa tgaatttttg aaaaaatttt ttcatactaa atctcctaaa
    12781 aatattaaat aagtattcaa tcattttagc ttagtgatta ctgaaagtat ctgaaaaata
    12841 ggctccaaat acatctcata gtcttttgta agataatcta atggaaaaat agagacaaga
    12901 aaaaaaagaa aataaagact tttcagaaca atattttcct tctgactttt ttccttgact
    12961 cccttttaaa aaacaaacaa gtagttgata tgaattttaa aaaaattgat attaaagatt
    13021 tcctattagg tttccttaca tgcctttgtt tatttctact aacaagtatt gcaggatgtg
    13081 gtagaacact atctagactt ggtttaggtg atgaaaaatt aaatcttccg aaagatttta
    13141 aaacaatggt gagcgtgagt ttccataaag agtcaaatgg agacacaatc aaagatatga
    13201 cctatgaaac actggacggt aagtttagat ctgtagaata ccgagacaaa ctctggcata
    13261 tggaaggtgg gattacctgg gaaaaggata attaatatta accgctaatg cctaaaagta
    13321 gcttttcaat gtttaattaa cgttaaaagg ctttaaatta taaaatttaa agttgtatcg
    13381 atcagaaaat aattttaggc aacctaggaa ttttaaatgt tattcacaag tatagaattt
    13441 tttattttct ttttatttgt ttttatattt caatggtata tacatccatt gtttgcaaaa
    13501 gcaaatgatg agcaacaatc ttactccttg catatttttc tactcctagc gagttattat
    13561 ttttatatga gttgggatta tagatttggt gcgttaattc taatatcaac tctaatcgat
    13621 ttcttcttgg caattcaaat tgaaaataca gaagttaaaa gtaaaagaaa attctatctc
    13681 gtaattagtt tattcttaaa tttaatttgc atcttaggtt tttttaaata ttataatttt
    13741 ttcgcaagat cgattaattc tacagcggga aactttggtt attcaagttt ctttccaatt
    13801 ttaaatataa tactacctgt aggtatttct ttttttacat ttcaatcact cagctacacg
    13861 atagatgttt acagaaaaat aattccagtg gagagaagtc tcgtaaagtt tgcactctat
    13921 gtatcctttt tccctcaact agtggcagga ccaattgtta cggcaaagac ctttttgccg
    13981 caactcttca ggcaagttaa acttgaagag gtcaacttta aaaaagcaat tcgatatttc
    14041 ttactggggt attttaagaa agttataata tcagataaca tatcacctat cgtagattta
    14101 atatataaaa atcctgaaaa ttacggaaca gaagcattgt ggttagctac ctccttattt
    14161 gcaatacaac tctatggaga ttttagtggt tatacagata tggcttatgg ctctgccttg
    14221 cttcttggct acgagctacc ggaaaacttt tttatgccat tcaaagcagt tagtgtttcc
    14281 gatttttgga gaagatggca catatcgctt tcaacatggc ttagggatta tatctatatt
    14341 tcactggggg gaagtcgaaa tggatatttt cgtcataagt ttaatttatt tatgacaatg
    14401 tttctaggtg gaatttggca tggagcaaat tggacttttt ttatatgggg aaccactatg
    14461 ggattattcc ttgcaataga aagcatgttc aatgattgga aagaaaagaa tgcgaaagat
    14521 ttttttcaca gatttagaat tccaattaat ataataggtg gaatatatgc gttaggagca
    14581 gtaagtataa cctgtgctct gtttcgtgga gaaagcttag aaaaatccct tttaatgctt
    14641 cataacatgg ttcactttgt tccaggttcg ccacgcccat atatgattaa aactgcttta
    14701 ataatgtttg tcattgtatt tttaggacac tcgattggtc actatttata tgaaagaggt
    14761 aaggttttta aaattccata ttggttagaa gtaagttttt atccatttat catattagga
    14821 attgcatact ttacaaatga caatgaagca ccttttgttt attttcaatt ctaacttacc
    14881 taacgctttg aggtaaacct gtattcagtg gagaagtagt atttgcaggt gttgttgcac
    14941 cagttggtgg tggatttgtt ggagtagtcg ctggattaaa cgggtttact cggtcttcac
    15001 caataatact tagtttagtt ttttccactt tttcatacaa tttatcttga atatttcgaa
    15061 atacaaagga acatttttca ggattagaat aatcctcttt ataaagaaac atagaatcca
    15121 aaaaccaaga aaattctcct cgattcataa gaggattttt agattgaact ggatcagttt
    15181 ttttcttatc aaaaatactt gcctcttctg atgattttgc attttcattt cttttcagtt
    15241 cttctgattt ctctttgtat ttttcagaat cggagagacg atctttttca agacttcgtt
    15301 ccttatcatt ccacttatct tccattgcaa tttttttgag taggggtaaa acgatagtat
    15361 ctcttttacg aattgcatct tttagacaag atttacgttc ttccaaaata gtaagctcct
    15421 ctttcgtaat cggaacctct acaacagctt gaaaaaaatt tcgggaaata aaaccagtat
    15481 actcagtatt gagaagtggc tttgttctat agtttggatc tgctggctta caattaaaaa
    15541 gaatcaaaat aataattaga aagaattgca taaggaaaga atccagtttg aaaattcttt
    15601 gtcaagatgc ttttctttca ttgggataga agtccattta tactaactag aaattcttgt
    15661 ataatttgta aatactgaga aaaatattat cacctcagca aatccatttt ctttccctga
    15721 tctaaaactt tatatgattc aaattttaaa tatgaggata atgtatatta tcctcatatc
    15781 tagaaaaaag agtagcttat ttggtctcat acggaatgca ctattttttg ctacacaaaa
    15841 ataaatcaag caatttttgg catcaagagt tttataacta gtaattcata ttaaggattt
    15901 ttgcaggtta tagaacaaaa cgaccactca cctatggatt taaaagaatt taggagtcga
    15961 atgatgtagg ttctttttca aattatacga tctaacattt ttctttcatt acgattgcaa
    16021 gttgacttac aagtttatta tgggaaattg attatcatgc gagtaaattt tattgttata
    16081 ttcttaattc tattttctta ctctgacctt ttttctcaaa aaaagacaga aactgtttct
    16141 ctcaaaggaa atgaaatggg tgcaaaacga tgggcaatta tcgttgggat caacgaatat
    16201 tcagacaagc aaattagctc tttacaaaaa gctagaaatg atgcaaaact cattgcacaa
    16261 atcttagaag aacaaggtca atttgaggaa atccatttaa tgaccgatga cctatcgagt
    16321 aaaaatcctc tctatccaac taaatcgaat attgaatcaa aaattgacca tgttctagat
    16381 ttttcctctc cggtagacac aattcttttt ttcttctcgg gtcatggaat ttctgattca
    16441 aatggaaatg gctatctttt aacggtcgat agttccctag acaaatcctt attaacctcg
    16501 cttacagtaa atacgattat caaaaagatc aaagaccgaa atgtcagtaa atcgattcta
    16561 tttctagacg cctgtagaga tgttacaaat tctaaaagtt ttttaaaaga aggcttaaag
    16621 tctgaaaaat cctcaagtga agattttcca attctatttt actcaactac aactggatat
    16681 tttagttatg aagattctaa atctagcttt ggagttttta caaaatattt agcacatgga
    16741 atggaaggaa aagcagatac aaatcaggat ggaatcgtta gttttggaga actggtagat
    16801 tttattcaaa ctggagtcgt tggttggtct gatcaaaatg aaaaagaaca aaaaccctat
    16861 gttgcttaca acagagaaaa attcggaaaa attcctatta cggcaagtgt ggataaactg
    16921 acgagtcttg tagatgaaaa tagttttcca aaatccaatt caaagctagg tgctgttatt
    16981 cgctcatttg tgactccagg gtggggacag tggtatatgg gagcaagtga gaaaggtctg
    17041 agttattttt ctatctttct agttttactt ggcaatgcgg cttatcagta tcaaccctat
    17101 caaaaagcaa aagcaaatta tgaaagcacg atcttaattc cagcaactcc aggaggaggt
    17161 gatacattgg gcatcaatta tcttctcttt caacccaagt tggatgcagt caatcatgca
    17221 aaatacaatt ataatttatc catcggagcg gtcggagtat tttgggcttg gaatctgctt
    17281 gatttatttt tattcaaagg aaataatttc ttttgggcgg taaattttcg cttaacaccg
    17341 gtggataatt caactattta cgctcattca agtattcagt tggatcaaaa aaaagagatc
    17401 gtgtttacac tgaggttcta aatgaaaaat aaatttctta atgcgttagt ttttatctat
    17461 ctaatcttta atttttcaag ttgcgtggga gctgttaaaa gaagtattct agatccaaat
    17521 agtttcttag gattttttct aagccttgat ccatttaaat taaacccgcc tcctaagagt
    17581 aaaattacac ttttaaatac aattagttct attcaagaag gggaatctac gaccatggga
    17641 attctactac agaacaaccc ttataagcaa atcactgtta cctgctctac agattatcct
    17701 tctcttttga tcaacggtgc tagatctaca actttcgtat ttgatagcag taatttttct
    17761 acacaacaat cgttaaatct aacaactgtt acagacaatg ataatacaag tgacaatgtg
    17821 accctctcct gttccgctac cggattagat acagtaactg catcgatcac agtatcagac
    17881 aacgcaggta ttattttaag tggaacagtt cccactctat tagagggtga tacagttacg
    17941 ataggcgtaa gtctttccag agcccctact accaatgtta cggtcaatgt atctttaaac
    18001 aatagtgcag ttactattaa ttcagcaacc accctaagtc ttacctttac tcctagtaat
    18061 tataatacat cacaaagtat tacaattctc tcactcattg atggaaattc tactaacgat
    18121 ccgacaactc ttacttttca aatgactggt atcgctgatg tgacttacaa tctaacaatt
    18181 atcgatgata acgttattca acttacaggt cctcctactt ctattacaga aggcacatct
    18241 acccttatgg ggattcgtta ttttaaatcc cttttaacca ataactccat caccattacg
    18301 agtagtaacc cggctgcgat tactgtaaat ggaggtgcat ctgctacttt taattatact
    18361 cctggtaatg ctaccacaga tcaaaacgtt actcttgcag cagttctaga tgcaaatctc
    18421 gtatcggaaa ctgtaagtct tacacttacc acaacaggaa tggatccact tgtaattgtt
    18481 gtaactactg ttgatataga tgtccaaaac atggtcatta caggtgcgat tccaacgtta
    18541 accgaaaata tgaatggaag tattttagtt cacctaacta atttaccccc taccaatgtc
    18601 accgtaaata ttaccacaag caatgctagc tccctaacag tctcaccttc aacccttacc
    18661 tttacaccag gtaattataa tatagatcag gcagttactg tcaatgccat tgatgatgca
    18721 aacgaaacag ccgagtatct cactgtaatt tttacaagca cgagtgctcc catgcaaatt
    18781 tacaatataa ctactattga tgatgatact cgtattttac taagtggtgc aagttcagta
    18841 aatgagggag gttcctctgt tgtaaccgtt agtctctctg gggatcctgg aattcctcgc
    18901 acaatcaatt taacttctag taatttactt gcacttacac ttggaaccgc atctctctct
    18961 ttcagttcag gcaactactc gcaatctatt tctatcaatg gagttcaaga tgcaaatata
    19021 gtaagtgaag ctgttacgat atccgctaca tctgctggtt taatttctgg aagcacctct
    19081 ataaacacag tagatgacga cacaatgaat attttactca cagcaggttc gaatcttgtg
    19141 agtgaaggat ctacttccac tttaaacctg agattaacac aagaaccatc cccttcgtta
    19201 actgttactc tgaattctag tattgcagga tccgtttcat tgagtccagc tagtttcaca
    19261 ttcgacaatg tatgtccagg acctcaatgt tggagcacaa atcaagttgt gactcttaca
    19321 ggtgtagaag atgtgaatca aaccagtgaa acgtcagtca tcactgcctc agctcccgca
    19381 gtcacaaacg tgactcaaaa tattacaaca attgaaaatg atacgactcc tgtatttaca
    19441 ggtgcaacga ctgttagtga gacaggaact tctgtaataa ccgttagtct atctggagac
    19501 ccaggggcga cccgcactgt aagcttggtt tcttcaaact tacttgcaat gactgtctct
    19561 ccttctactc ttacttttaa ttcggctaac tggaatatac cgcaaactat tttagtcgca
    19621 ggtgttccag ataacaatat tatttccgaa tctgtcacca tcaccggtag tggagttgga
    19681 ttaatcacat cgacaaccac tgtcaataca gttgacacaa caaccatggc gattacactc
    19741 tccggaaatc cgacgagtat cg
//
LOCUS       DAOQOS010000102        19735 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_1367_length_19735_cov_6.033130, whole genome shotgun sequence.
ACCESSION   DAOQOS010000102 DAOQOS010000000
VERSION     DAOQOS010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 19735)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19735)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19735
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1367_length_19735_cov_6.033130"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            119..2188
                     /locus_tag="PLF66_18930"
     CDS             119..2188
                     /locus_tag="PLF66_18930"
                     /inference="COORDINATES: protein motif:HMM:NF012697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66173.1"
                     /translation="MKILQNSFIKLNSISILLCIALFKILLFFLFSSEYSNVLFIPFL
                     VKFANQILTFQFISPWADSIPVRVDAFPYHPSMLYLYTLFQLPIALFKLGSFAQSIGF
                     IIPTLLADLLIFRVMLQFFPDRERIILWFYGLSPVILYSIYIHGQLDLWPTALLLLSV
                     SRIFKGNYKESAFYFGLAMTFKLHIIAVLPLFIVYIFRKERNKSLFNFFSIVFLIYGL
                     ISFPWYFDSGFRTMVLFNSRQDLIWKVFYKVGDNSIYLSIAAILILYLRFLGYTKLNK
                     DLFITWVTILFAVFVLFIPPAPGWYVWFTPFLLYFYFKYLKKQYQIFGLYGFFCISYV
                     LFFLFVWKGDYIDLFFLGKEVNLKSMNPKFSGLLYTFLQTSLAANLFIIYRIGVRSNR
                     IYKKPNSILIGIGGDSGAGKSSLLHSLEKLLSPSVTLLEGDGDHRWERGNLNYQTITH
                     LNPRANYLERQAENLIRLKKGEVIYRPDYDHDTGKFTEPQPVQPSDFIILSGLHTFYL
                     PKMRKVIDIKIFLDTEENLRLHWKILRDTKKRGYSPKKIQEQIAKRAKDSDKYIQPQK
                     NFADLTIEYFSRNKFKVGDQAANPHISLKISISSEYHMEDLILRLEEGGWLSEWDYGQ
                     DLKSQYLIFDHHPSPEELSEMASIYIPVLEEIISPFQDWENSLQGIVQLFVLVMISKK
                     YLEFDNA"
     gene            2181..2858
                     /locus_tag="PLF66_18935"
     CDS             2181..2858
                     /locus_tag="PLF66_18935"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HMZ66174.1"
                     /translation="MPKPYQYFFLDLDDTLYSYSICHESGLNGFVEFASTEFKLEKEK
                     VKETYKQVRKEINTRLHGLASSHSRLLYAKEFCEAFGLNPVQYAIPLENSYWKNYFPN
                     MVLGEGVIEFLQELKRREKKIGLITDLTTEIQLKKLAFLKIDSYFDAIITSEEAGAEK
                     PSSRIFELAIKKLNAQKLSSIMIGDNYEKDVLGARDFGISSYYFGFREDEELCLPSFR
                     EALDKLL"
     gene            2855..3913
                     /locus_tag="PLF66_18940"
     CDS             2855..3913
                     /locus_tag="PLF66_18940"
                     /inference="COORDINATES: protein motif:HMM:NF012804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HMZ66175.1"
                     /translation="MIADNSVLADLLKMSRRAGERFDLVQAGGGNTSAKLPNGNMYVK
                     ASGLRLSDLQSEKSFVLVDNLNLRNAFYKTDWTSFDKKQKEKQAGELVSSFNMTKDRK
                     PSIETLLHSILDGFVLHTHPVLVTSYFSGLDAKEKCDSLFSDVALVAYRTPGIELALE
                     MIDSLKSFQQKKQKDPKAFIFQNHGLVTFAEKAEDAFQKTEEICNLLGKEFNFAEEKI
                     ANHISNAIESITKEAWVTIYSPKSESNSISINQKPFFPDAVVFLAAGVLVLVSIEPKK
                     IVEQIQLYLDTHTIVPRVFYFQNSLYFSGNTYSKARESEEVWRLHHIASQKNPNSLSD
                     SEIKYLSHWEAEKYRQKV"
     gene            3915..5474
                     /locus_tag="PLF66_18945"
     CDS             3915..5474
                     /locus_tag="PLF66_18945"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HMZ66176.1"
                     /translation="MHIIVPMSGIGKRFLDAGYKDPKPLIQVEGKPIIAHVVAMFPSE
                     TKFTFICNEEHFANTSMRSVLLSLVPNANVISIPPHKKGPVYAVSLIYSMIDDDEEAI
                     VNYCDFGKDWDYKAFLEDVRSNQADGAISAYRGFHPHMLGTVNYAFMREKDNWMLEIQ
                     EKKPFTNDRMSEYASDGTYYFKSGRILKKYFDLTMERDLSVNGEYYASVVYNLLVQDG
                     LKVRIFEINHMLQWGTPGELKEYLYWSDIFKGLSKKYETAPAQDRINLIPMAGRGSRF
                     ADQGYKDPKPLIPVDGEAMVVQAGKSLPASKRNIFVCLNEHLQKYPIQTTLEASFENV
                     KVVGIQGVTEGQAITVSLGLSEENIDKPLFIAASDNGMLYDGIQLEKFIAEEDPDVFA
                     FTFSGNPTSERNPQMYGWIQASGKDALAVSVKVPISNNPIQDDAIVGAFYFKTARIFL
                     QILDVLIQKNIRVNHEFYVDSMIGLAIELGLKTKIFRIDHYICWGTPNDYKTYLYWQK
                     FFKKVDWHPYK"
     gene            5471..5833
                     /locus_tag="PLF66_18950"
     CDS             5471..5833
                     /locus_tag="PLF66_18950"
                     /inference="COORDINATES: protein motif:HMM:NF016060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HMZ66177.1"
                     /translation="MKRELIFFLISGCSAVLTDLLSYYFLFNILGHNVSKTISFILGS
                     IVAYILNKYLTFQKKEPSSLEILRFAILYALTLSANVFINDTVLKILLDQKLFAFLCA
                     TGTSTILNYIGQKFWVFK"
     gene            5830..6534
                     /locus_tag="PLF66_18955"
     CDS             5830..6534
                     /locus_tag="PLF66_18955"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HMZ66178.1"
                     /translation="MKKLTIVIPCYNEEKNIPLILERLVKVVKRDDIEFLLVDNGSTD
                     GSKEVLEKLIPKYNFAKTIRVPVNQGYGFGILQGLAATDSLYIGWTHADMQTDPYDII
                     RGLEILEKEETKDCYVKGSRTGRPIFDQFFTIGMSFFETLYMGKPLWDINAQPNIFPR
                     TFFESWKNPPHDFSLDLYALYQAKIQGLKVLRFTVVFPPRIHGQSSWNTGLKAKWKFI
                     KRTFDFSIKLKKELKN"
     gene            6531..7133
                     /locus_tag="PLF66_18960"
     CDS             6531..7133
                     /locus_tag="PLF66_18960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66179.1"
                     /translation="MKTEYIAHRINTVNELLSLPVGVGVELDLRDEGKDLILQHDPFT
                     GGEKFETFLEHYGDRNTLILNIKSERIEARVLELVKKYNVQKYFFLDSSFPMIIQLIR
                     MGEKNLALRFSEYETLDTILTLAGKVDWVWVDCFTKLPINSENYKILKDNGFKICLVS
                     PELQGRQEDILIYKEYLEKENISFDAICTKIKNFPLWQKD"
     gene            7144..8703
                     /locus_tag="PLF66_18965"
     CDS             7144..8703
                     /locus_tag="PLF66_18965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66180.1"
                     /translation="MIRSRLNKYLPSILFVISFVIIFITALIKFPSNGIEAEFFAEAG
                     ANFFYHARYTPVFKTFLIPEAGYLPLLQRIIAFFVVTIGGAEANAPFIFQVIALAGIS
                     FFCSFINLHHFKILIPSNIIRFALGLTLAFFPNFEMFPYINFIYFGFVFLFLLSFYGF
                     RNKSYLTTFLWGLLGLFLIGSKALLLCIAPYFGLLLLMAVYKNKRKDALVYSMLTLGA
                     ILQSVFCLYVLIFQSGGVQSSSQKNISQLITMTFDLYLGISVQDFMGYFYLIPSFFFI
                     KIALFLTAFLFIPIYFYRINKKQLAFGLFGLQLTSIISLVLFLGLAPVEYYPKLKIGE
                     FSHPPFFRWYFIAHHSFILTVFILIGNLRSKIMQLILIALFIYFYPGYRLFQEQEDIY
                     KNKDFSHSQWDSFIPLLKEDAFCIPVNPDPWLIQHNCEVSNSPEERVVLAVKVNMLNS
                     GMKPEKLILEYSDKSRVDAKNILKDTIYYQYWVVEKKEANIKFHFYNLSGKEIFPNKI
                     VYYSFTPKKSY"
     gene            8703..10265
                     /locus_tag="PLF66_18970"
     CDS             8703..10265
                     /locus_tag="PLF66_18970"
                     /inference="COORDINATES: protein motif:HMM:NF021436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2142 domain-containing protein"
                     /protein_id="HMZ66181.1"
                     /translation="MIMMFSKINYRHFVWLLCILTGLRIFVGNAAFPFFNNVDEHAHF
                     DTIMKYAKGDLPSKENLRFEPESARFIVLYGSPEYFYRAENYTSKEIPLPNWKLGEAV
                     NVDYLSQEWLDKKNHEVFSPPVYYSFLGFWFHVGKWMGLEGGYQLYWLRFLNLFIYIS
                     IFWIVYWMLGVFWHDNLYIRTSVLLLVTVFPQDVFYTLNSDTLSALFCLLSFFFLMEI
                     YFTNKSYLFYFLAGSFTAMAFLTKVSNVPLITLVGLILFLRVIKLIRSQQMIQKEIVN
                     LIIFVLSTSIPVFVWFLWNQMTIGEFTGTAEKMRDLDWTYKPITEIFNHPIFTLKGFT
                     YFFSSLFKTFWRGELVWGLREMAAKDSDRLYTYSILLVIASGFNSILNKQAYSDRIRF
                     VQILSFIYIIISVLFLLLLSMIYDFGKCWYPSRENPFFTSGRLMIGMMIPFLILFVDG
                     IRIFILFIENLLNWILSFINKRFSDSKTKIQLSTTKVLIGFILLICFLISQYETKSLW
                     MVMQSKYNWFFL"
     gene            complement(10366..11517)
                     /locus_tag="PLF66_18975"
     CDS             complement(10366..11517)
                     /locus_tag="PLF66_18975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008594817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HMZ66182.1"
                     /translation="MNKKNRKFIIPISILFIGTLLSPLFYCLDANKSSLALNAKTEVN
                     PSPALSQAIGMQSAFNEVFEHTADSVVSIATEKTVNIQMNPFMDPFSGEPFFFGPRGK
                     GKTQKQKQNGLGSGIILNKEGYVLTNEHVIHETDKLHVKLRNKKSYEAEIIGSDPVSD
                     IALLRIKGAKDLSPIDLGDSSKVKVGDWAIAIGAPLGYEHSFTVGVVSGIARGGIDSS
                     GVSYIQTDAAINQGNSGGPLLDIYGRVIGINRMIVSPSGGSIGIGFAIPINEAKRIVE
                     ELKTNGKIKRPWVGIALDAIDDETKDELKLSSTEGALIKQIYGDSPAAEAGLELQDVI
                     TKINDEKMKSPEDVVNFVRKSKIGDKLSFTILRKGKEVKIMVKIKERTQ"
     gene            11675..13351
                     /locus_tag="PLF66_18980"
     CDS             11675..13351
                     /locus_tag="PLF66_18980"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HMZ66183.1"
                     /translation="MNEKNLLEVMSHVDYLEDKSNSLDLNSVKSLNFKKNYSGSINFG
                     LSNSTYWFRLKIKNPTSSPIENWFFFIEYPQLDEVVFYIPMKNDSYFEKSIGDIYPFQ
                     DRDMKIRFLNLRLPNGFKEGEDVFIKVKSVPAITIPLFVGTEIAFRNKDHVRQMSIGS
                     YIGILFIMALYNLFLFIILREAAYIYYVLFVLSVCLYVISFLGLGYEFIWSNYPYLQS
                     HMILISSCVMLFNLCLFTISFFDTKTYAPKIHIYLIYSLVIFPLTIIFILISNSILKV
                     YLTSTLGIIYSFLLPLIGFYVWKKGNRFARVYLFAWIPFFMAANLFILKTFGIIDKYA
                     LEINYLVQFANIFEAVVFSFALADRVRVLQKENEIAQLEMLDTMKKDIDSFRLLQAAN
                     KLANTDSLTGLHNRRYFYTLVETLWRKPDPLIPDMSMLVIDVDHFKKVNDTYGHDVGD
                     QVLKQVAEILSKSIRNMDIVGRYGGEEFLVFLPKTNIQEAIELAEKIRVSIQEQKILL
                     SENQILQITASIGVASRVEKEMEIDQLVKSADTMLYKAKESGRNRVVGFS"
     gene            complement(13344..15422)
                     /locus_tag="PLF66_18985"
     CDS             complement(13344..15422)
                     /locus_tag="PLF66_18985"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HMZ66184.1"
                     /translation="MFSKKIILAFILPSFLSIFFSSCIKPKIEKQIPKAIKGVIDLRE
                     IDFSSPFSDSIQLDGEWAFFWNEFIDPKTSDWENKVNSYIQVPSQWQNQKDPSGNFYP
                     AYGYASYVLKVILPEKHPNLYFSMSDTGMAYSLFVNGSLRASNGKVGKSADEMFVRQQ
                     YIIFPAIKTTDTELIIVIHNSNFHYYKAGVWQSVVLGEAKTIHEREFHSIAIDLIVIS
                     ALLIMGLYHIGLFFNRRKDISPFYFGLFCILVVLRTIAIKERLIYEIWSDIPFMIVHK
                     IEYFSFYYGSLVFLQFIHSIFPEDTNPRIYKVFKTIFILCSILVLVSTMSIYTRTLIF
                     VQLTILVGIVYTLKIIFYAIRNKHVGAKSFLAGLLIFFVTIIHDILRTRGVVYSPFLA
                     SYGLLFFVVSQAIVLSRRFANAFVLSEALTEELQILSSQLEAKVNERTKELNLALKSI
                     QQDLFYAKRIQNNSLVIDYSEIKDLEIVPYYSAMVEVGGDFYGISSPVKDKYRVFLAD
                     VTGHGIQAALITMAVKGIYDNIKNFDLAPSHALDIFNNEFLEKYQSLNSLLTCIFLDI
                     DPKNSKIQYASAGHPSCILLSNGKIELLEKTGRMVGITKNNKYNDLEKEFHKGDRLYL
                     FSDGIYEQFNSSLEEFGEERLYSLIKDTRDLPLKDSIQKIIADLTEFLGTTKIQDDIT
                     IIGIESTNSN"
     gene            15634..16830
                     /locus_tag="PLF66_18990"
     CDS             15634..16830
                     /locus_tag="PLF66_18990"
                     /inference="COORDINATES: protein motif:HMM:NF019224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1554 domain-containing protein"
                     /protein_id="HMZ66185.1"
                     /translation="MKEIGKISFRIVIITFLIELAFSCTVRPKTKILGLFSLVNREVT
                     ISYNLGGTVSGLQGTLILQNNMMDRITIEANGNFTFTNLYPTNSNYSVTVFSQPSSQT
                     CSITNGSGTILSSSISNISVRCGNTISSLSSLTISSGTITPSFSSSLYSYSFSVLNSA
                     SSITITPTATDASATITVNGSSVLSGSSSSPISLSVGSNTISIVVKASDGITTSTYTL
                     TVIRSTLNAYRIFITASTYDGNLIGTSSSGYLGADSKCNSDANKPTDGSIYKAVITDV
                     VNRSACSININCTNPSENLDWALRTNTSYARTDGTALFTTNAAGIFVFGTLTNSFGGG
                     QYWTGISTVSSWRPSTGGNNCSEWSSNSGALNGNYGDGSVTNYTSIAMTSGACNNLKS
                     LLCAEQ"
     gene            17012..18340
                     /locus_tag="PLF66_18995"
     CDS             17012..18340
                     /locus_tag="PLF66_18995"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HMZ66186.1"
                     /translation="MLKNKVYLIVFSLVVGVLFYFLYNSDIQKKTHSHNDSPNLSGEK
                     IKFLVTITQARAARALADWYTKETKAVVQITEIGYDETYRKTLEDIRSSDPQYDIFII
                     WYPELGDLIRNKALLDLTDWIEENKETIQPQDFIPRIYDAYTLQKGRRWALPFDGDTH
                     VLFYRKSILSKHNLNPPETWDDYLNVSRTITEKEKVNGIYGTAIMSYKAPILILSSFL
                     NRVGGYGGQLLTKDGRPSLDTPEVKMALESLLEQAEYALPSPLETSFEVSRDSFLSGR
                     VAMVEQWTDIGVMAEDNSESTIRGDWGAIQMPKGSGKNAQHAPALNAGFALAISSKAP
                     NLEVAKDFLLFAVRPDIMAKYNVLNGGTDPTRISILKSDDYRKFAPEVSAAAEAALFN
                     ATPWPNIPGSRKLMQILSDNIVLALRKKISVENAISDTQKRWTEIIDSTN"
     gene            18397..>19735
                     /locus_tag="PLF66_19000"
     CDS             18397..>19735
                     /locus_tag="PLF66_19000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020769023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HMZ66187.1"
                     /translation="MLALTLGLSFATWYSFVQAQKGIGIEGYNVLSNQTEQFLKNQAQ
                     ERSRSLDLQFAQAQSVVSYGASEIALIVSEKQIDKKKILTALKNLFHSLGNSKAKVFF
                     YSKKSGLITYQENKQNKLEEEHVEFHSPNFFPGIHNFAKKSESVLWSKPHPSLYDEYE
                     WVVDIIAPLNRLNPYETFIGISFSLTDLITQFNQLHPTRGSYCFLMDENQKLVAAPPH
                     ARVELAPNDNSLKEEQIHLNGYEDKVLEQALKEMALGRSALVKVSIKGNEKYLAYHPL
                     EVMNWKVGIVVPVSIATSASIQLQRVVESEMKKSFYRIFIAAFFIFGISLLGGSFLVR
                     HLLVPLEQVSLASKSMLDGDFKQRVIVKSTDEISNLARVFNQMADHIQVLISHLEQRA
                     NELEELNQSLEAKVVERTSKLEERTIQLDKANKQLQKEMRDREEAQEALTIANS"
BASE COUNT         6337 a         3152 c         3411 g         6835 t
ORIGIN      
        1 gttttaaaat cctgctactt cagaaatccg aaccactcta ggtatcagaa ttaatttctc
       61 tttactaaaa cgggttttat ttcagagtga gtttctatat tacgcgatag ccaaaacaat
      121 gaaaatactg caaaattcct ttataaaatt aaattctatt tcgattttac tctgtattgc
      181 gctctttaaa attcttttat ttttcctttt ttcttctgaa tattccaatg tattatttat
      241 cccttttcta gtaaagtttg caaatcagat tctcaccttt cagtttattt ctccttgggc
      301 agatagtatt ccagttcgtg tagatgcatt tccctatcat ccttccatgc tctatctcta
      361 tactttattt caactaccaa ttgctctttt taaactagga agttttgctc agagtattgg
      421 gtttatcatt ccaactttac ttgcagattt actaatattc cgtgtaatgc ttcaattctt
      481 tccggatcga gagcgaatta ttctttggtt ttatgggcta tctcctgtta tcctctactc
      541 gatttatatt catgggcagc ttgatctatg gcctaccgca cttctacttc tttctgtttc
      601 tagaatcttt aaaggaaatt ataaagaatc tgctttttat tttggcttag ccatgacgtt
      661 taaactgcat atcatcgcag ttcttccttt gtttatcgtt tatattttta gaaaggaaag
      721 aaataaaagt ctctttaatt tcttctcaat cgtatttctt atctatggtt taattagttt
      781 tccttggtat ttcgattctg gttttagaac tatggttctt tttaattcta gacaggatct
      841 aatctggaaa gttttttata aagtagggga taatagtatt tatctctcga ttgcagccat
      901 tcttatttta tatctccgat ttcttggtta tacaaagtta aataaagatt tatttatcac
      961 ttgggttacc attctatttg cagtgtttgt attatttatt ccacctgctc ccggttggta
     1021 tgtttggttc actccattct tactttattt ttattttaaa tatctaaaaa agcaatacca
     1081 gatttttggt ctctatggtt ttttttgtat aagctatgtt ttgttttttc tatttgtttg
     1141 gaagggagac tatatagatt tattctttct aggtaaggaa gtaaatctta aatctatgaa
     1201 tccaaaattt tctggtctac tttatacctt tttacaaaca tctctcgcgg ctaacttatt
     1261 tattatttat agaattggag ttagatcgaa tcgtatttat aaaaaaccaa attcaatctt
     1321 gattggaatt ggaggggata gtggggcggg taaatctagt ttgcttcatt ctcttgaaaa
     1381 acttttgagt ccttctgtta cacttttaga aggcgatggg gatcacaggt gggaaagagg
     1441 aaatttaaat tatcaaacca tcacccactt aaatccaaga gccaattatc tagagagaca
     1501 agcagaaaat ctaattcgtt taaaaaaagg ggaagtcatc tatcgtccag attatgatca
     1561 tgatactgga aagtttacag agcctcaacc tgttcagcca tccgatttta ttatactgag
     1621 tggacttcat actttttatc ttcccaaaat gcgtaaggtg atagatataa aaatattttt
     1681 agatacagag gaaaatttac gacttcattg gaaaattttg cgagatacaa aaaaaagagg
     1741 atacagtcct aaaaagatcc aagagcaaat cgcaaaacga gcaaaagatt cagacaaata
     1801 cattcagcct cagaaaaatt ttgcggatct tactattgaa tatttttcaa gaaataaatt
     1861 taaagttgga gatcaagcgg ctaatcctca tatatctcta aaaatatcca ttagttccga
     1921 atatcacatg gaggatttga ttcttcgttt agaagaaggt ggttggttga gtgaatggga
     1981 ttacggtcaa gatcttaaaa gccaatactt aatctttgat catcacccaa gtccagaaga
     2041 gttaagcgaa atggcatcta tctatattcc tgttttagaa gaaatcattt ctccttttca
     2101 agattgggaa aactctctac aaggaattgt tcaacttttt gttttagtta tgattagtaa
     2161 aaagtatctg gagtttgaca atgcctaaac cctatcagta tttcttttta gatttagacg
     2221 atacgctcta ttcctatagc atctgccacg aatctggtct aaatggtttt gtagaatttg
     2281 cctcaactga atttaaatta gaaaaagaaa aagtaaaaga gacctataaa caggtcagaa
     2341 aagaaatcaa tactagactt catggacttg ctagttcgca tagccggttg ttatatgcaa
     2401 aagaattttg cgaagcattt ggtctgaatc cagttcaata tgcaattcca cttgaaaatt
     2461 cctactggaa aaattatttc ccaaatatgg ttttgggaga aggcgtaatt gaatttttac
     2521 aagagctaaa aaggagagag aaaaaaattg gtctgattac tgatttaacc accgagattc
     2581 agttaaaaaa gctcgccttt ttgaaaattg attcctattt tgatgcaatt ataaccagcg
     2641 aagaagcggg agccgagaaa cctagctctc gtatttttga attagcgatt aaaaaattaa
     2701 atgcacaaaa gctttcctct ataatgattg gagataatta cgaaaaagat gttctaggag
     2761 ctagagattt tggtatttct agctattact ttggattcag agaggatgag gagttgtgct
     2821 tgccttcttt tagagaagcg cttgataaat tgttatgata gcggataaca gcgtgttagc
     2881 tgatttactt aaaatgtcta ggcgtgcggg agaaaggttt gatttagtcc aagctggtgg
     2941 agggaacaca tctgctaaac ttcccaatgg aaatatgtat gtaaaagcct cgggccttcg
     3001 tctttccgat ttacaatcag aaaaaagttt tgttctagta gataatttga atctaagaaa
     3061 tgctttttat aaaaccgact ggacttcttt tgataaaaaa caaaaagaaa aacaagcagg
     3121 tgaattggtt tctagtttta atatgacaaa agaccgaaag ccatctattg aaacattgct
     3181 tcactcaatt ttagatggat ttgttctaca tactcatcct gttttagtaa catcttattt
     3241 tagtggactg gatgcaaagg aaaaatgtga ttctcttttt tctgatgttg ccttagtcgc
     3301 ctatcgcact cctgggatag aattagcact tgagatgatt gattctctta aatccttcca
     3361 gcaaaaaaaa caaaaagacc cgaaagcatt tatctttcaa aaccatggtc tagttacatt
     3421 tgctgaaaaa gcagaagatg cttttcaaaa gacagaagaa atttgcaatc tcttaggtaa
     3481 ggaattcaat tttgcagaag aaaaaatcgc aaaccatatt tctaatgcca tcgaatctat
     3541 tacaaaagaa gcatgggtta caatttattc tccaaagtca gaatcaaatt ctatctctat
     3601 caaccaaaaa ccattttttc cggatgctgt tgttttttta gctgctggtg ttttagtttt
     3661 ggtttcaatt gaaccaaaga aaatagttga gcagattcaa ctttatttag atactcatac
     3721 gatagttcca agagtttttt attttcaaaa cagtttatat ttttctggaa atacttactc
     3781 aaaggcaaga gagagcgaag aagtctggcg gcttcatcat attgcttccc aaaagaatcc
     3841 aaattcactc tctgattctg aaataaaata tctatctcat tgggaagctg agaaatatag
     3901 gcagaaggtg taatatgcat atcattgttc ctatgtctgg cattggaaag cgtttcctag
     3961 atgctggcta taaagatcca aaaccattga ttcaagtcga aggaaaacct attatcgcgc
     4021 atgtcgtcgc aatgtttcct agtgaaacca agtttacttt tatttgcaat gaagaacatt
     4081 ttgcaaatac tagtatgcga tctgttcttt tgtctttagt tccaaatgct aatgtgattt
     4141 ccattcctcc acataaaaaa ggtcctgtct atgcagtatc tcttatttac tcgatgatcg
     4201 atgatgatga agaagcaata gtaaattatt gtgatttcgg aaaagactgg gactataaag
     4261 catttttaga agacgttagg tcaaatcaag cagatggtgc tatttctgct tatcgaggtt
     4321 ttcatcctca tatgctagga accgtaaact atgcatttat gagagaaaaa gataattgga
     4381 tgcttgaaat ccaagaaaag aaacctttca caaatgatcg aatgagtgaa tatgcatcgg
     4441 atggaacgta ttattttaaa tctggaagaa ttcttaaaaa atacttcgat ctaacaatgg
     4501 aaagagattt atctgtgaat ggcgaatact atgcaagcgt tgtttacaat ttgttagttc
     4561 aagatggact caaagtgcgg atattcgaaa ttaaccacat gcttcaatgg ggcactcctg
     4621 gagaattgaa agaatactta tactggtctg atatttttaa aggtctttct aaaaaatatg
     4681 aaactgcccc cgcacaagat cgaattaact tgataccaat ggcaggtaga ggaagtagat
     4741 tcgctgacca aggatacaaa gacccaaagc ctttgattcc tgtagatgga gaggcaatgg
     4801 tagtgcaagc aggaaaaagt cttccagctt ctaagcggaa tatatttgtt tgcctcaatg
     4861 aacatttaca aaaataccca atccaaacaa ctttagaagc ttcttttgaa aatgttaagg
     4921 ttgttggaat tcagggagtt acagaaggac aagccattac tgtttctctc ggtctatctg
     4981 aggaaaacat agataaacct ctttttattg ctgccagtga taatgggatg ctatatgatg
     5041 gaattcagtt ggagaaattt atcgccgaag aagatccaga tgtatttgcc tttacattct
     5101 ctggaaatcc tacaagtgaa agaaatcctc agatgtatgg ttggattcaa gcgagtggta
     5161 aagatgcatt agccgtctcc gtaaaagttc ctatttcgaa taatccgatt caagacgatg
     5221 cgatagtagg agctttttat tttaagactg cgagaatatt cctacaaatc ttagatgttt
     5281 taattcaaaa aaatattcga gtgaaccatg agttttatgt agattcaatg attggtcttg
     5341 caatcgaatt aggtctcaaa actaagatat tcagaattga tcattatatt tgttggggaa
     5401 cgcctaacga ctataaaaca tatctctatt ggcagaaatt ttttaaaaaa gtggactggc
     5461 atccgtataa atgaaacgtg aattaatatt ctttctaata tcaggatgta gtgcagtctt
     5521 aacagatttg ctttcttatt attttctttt taatatactt ggtcataatg tttccaagac
     5581 tatctcattt attcttggat ctattgttgc ttatatttta aacaagtatt taacctttca
     5641 aaaaaaagaa ccatcttccc tagaaatctt acgatttgct attttatacg ctttaactct
     5701 atctgctaac gtatttatta atgatactgt cttgaaaatt ttattggatc agaagttatt
     5761 tgccttttta tgtgcaacag gcacatctac tattttaaat tatattggac aaaaattttg
     5821 ggtgtttaaa tgaaaaaatt aacaattgtg attccttgct acaatgaaga aaagaatatc
     5881 ccactcatcc tagaacgtct tgtaaaagta gtaaagagag atgatattga atttttactt
     5941 gtagacaatg gatcaactga tggatctaaa gaagtattgg aaaaattaat tccaaagtat
     6001 aatttcgcaa aaactattcg tgttccggtt aaccaaggtt atggctttgg catattacaa
     6061 ggattagccg ctacagatag cttatatatt ggctggaccc acgctgatat gcaaacggat
     6121 ccttatgata ttataagagg tttagaaatt ttagaaaaag aagagacaaa agactgttat
     6181 gtaaagggtt ctagaactgg tagacctata ttcgatcaat tcttcacaat cggaatgagt
     6241 ttttttgaaa cgttatatat gggcaaaccg ctttgggata tcaatgccca accgaatatt
     6301 ttcccacgga ctttttttga gtcctggaaa aacccacctc atgatttttc tttagactta
     6361 tacgcgctgt atcaagcgaa gatacaagga ttaaaagttt tacgttttac tgttgtattt
     6421 ccgccgcgta ttcatggtca gtcatcttgg aatactggtt taaaagcaaa atggaaattt
     6481 ataaaaagaa catttgattt tagtattaaa ctaaaaaagg aattaaagaa ttgaagactg
     6541 aatatattgc tcatcgaatc aatactgtta atgaattact atcccttcct gttggtgttg
     6601 gtgtggaatt ggatttgaga gatgagggaa aggatttgat tttacaacat gatcccttta
     6661 cggggggaga gaagtttgag acttttttag aacactatgg agatcgaaat acacttatct
     6721 taaatataaa atccgagcgt atcgaagcaa gagtattaga gttagtaaaa aaatacaatg
     6781 tgcaaaaata ttttttttta gattcatctt ttccaatgat aatccaactg attcgaatgg
     6841 gtgaaaaaaa tctagctctt cgtttttctg aatatgaaac tttagataca attctaacgt
     6901 tagccggtaa agttgattgg gtttgggtag attgctttac taaacttccc ataaattcag
     6961 aaaattataa gattctaaaa gataacggat ttaaaatatg tttagtttct ccagaattac
     7021 aaggaagaca ggaggatatt ttgatttata aagaatatct tgaaaaagaa aatatcagtt
     7081 ttgatgcaat ttgcactaag atcaaaaatt ttccactttg gcagaaagat tagagattgt
     7141 gttatgatcc gttctagatt aaataagtat ttaccctcta tattgtttgt aatttctttc
     7201 gttataattt ttatcactgc tctaattaag tttccctcca atggaattga ggcagagttt
     7261 tttgcagaag ctggagctaa ttttttttat cacgcaagat atactccagt ctttaaaact
     7321 tttttaatac ccgaggcggg ctatctccct cttttacaaa gaattattgc tttttttgtt
     7381 gtgacgatag gcggggcaga agcaaatgct ccatttatat ttcaagttat agcactagct
     7441 ggaatttctt ttttttgttc ctttatcaac ctgcatcatt tcaaaatatt aattccttct
     7501 aatataattc gatttgctct tggcttaacg cttgcttttt tcccaaattt tgaaatgttt
     7561 ccttatataa attttattta ttttgggttt gtcttcttgt ttctactttc gttttatgga
     7621 tttcgaaaca agtcttattt aacaactttt ttgtggggct tactaggttt atttttaata
     7681 ggctctaaag ctctccttct ttgtattgcg ccttattttg gattgttgct tcttatggca
     7741 gtatacaaaa ataaaagaaa agatgcgtta gtgtattcta tgctcacgct aggtgctata
     7801 ttgcagagtg tattttgctt gtatgtttta attttccaat cgggtggtgt tcaatctagt
     7861 tcacagaaaa atatttccca attaataaca atgacttttg atttatacct aggcatttct
     7921 gttcaagatt ttatgggtta cttctatctg attcccagtt tcttttttat taagatcgcc
     7981 cttttcttaa ctgcatttct attcatacct atttattttt atcgaatcaa taaaaagcaa
     8041 ttagcatttg gtttatttgg tcttcaacta acgtctatta tctctcttgt tttgtttttg
     8101 ggtcttgctc ctgttgaata ttatcccaaa ctaaaaattg gtgaattttc tcatccacct
     8161 tttttccgtt ggtattttat agctcatcat tctttcattt taactgtttt tattttaatt
     8221 ggaaatttac gttcaaaaat aatgcagttg attctaattg ctttatttat ttatttttat
     8281 cctggttata gactctttca agagcaagag gatatatata aaaataaaga tttttcccac
     8341 tcacaatggg attcatttat acctcttttg aaggaagatg ctttttgtat tccagtcaat
     8401 ccagatcctt ggttgattca acacaactgt gaagtatcaa attctccaga ggagagagtg
     8461 gtgcttgcag ttaaggtcaa tatgctgaat tcaggtatga aaccagagaa actgattctt
     8521 gaatattcag ataagagtag agtagatgct aaaaatattt taaaggatac aatttactat
     8581 cagtattggg ttgtagaaaa gaaagaagca aatatcaaat ttcatttcta taatttgtct
     8641 ggaaaagaaa tattcccaaa taagattgtt tattattcct ttacccctaa gaagagttat
     8701 taatgattat gatgttttca aaaataaatt accgtcattt tgtttggctt ctttgtatac
     8761 tcacaggctt acgaattttt gtaggaaatg cagccttccc tttttttaat aatgtagatg
     8821 agcatgcaca ttttgataca attatgaaat atgcaaaagg agatcttcca agtaaagaga
     8881 accttcgctt tgagcctgaa tcagctcgat ttattgtttt atatggttcg cctgaatatt
     8941 tttatagagc tgaaaattat acatctaaag aaattccatt gccaaattgg aagttagggg
     9001 aagcggttaa cgttgattat ctttcgcagg aatggctaga taaaaaaaat catgaagtgt
     9061 tttctcctcc tgtttattat tcttttcttg gattttggtt tcatgttggg aaatggatgg
     9121 ggcttgaggg tggttatcaa ctctattggc ttaggttttt aaatttattt atttatattt
     9181 ccattttttg gattgtatat tggatgcttg gagttttctg gcatgacaat ctttatatta
     9241 ggacatcggt tttacttctg gtgacagtat ttccacagga tgttttttat actctaaaca
     9301 gtgacactct ctctgccttg ttttgtttgc tttctttttt ctttttgatg gaaatttatt
     9361 ttacaaataa atcctatctt ttttattttc tcgctggatc tttcactgcc atggcatttt
     9421 taactaaagt ttcgaatgtg cctttaatca ctttagttgg tcttatttta tttctcagag
     9481 ttataaaatt aattcgctct cagcagatga tccaaaaaga aatagtcaat ctaattattt
     9541 ttgttttatc cacttctata ccagtatttg tatggtttct gtggaatcaa atgacgatag
     9601 gcgaatttac aggaacagca gagaaaatgc gagatctgga ttggacatat aaaccaatca
     9661 cagaaatatt caatcatcca atttttactc tgaaaggatt tacttatttt ttctcttctc
     9721 tgtttaaaac attttggcgg ggagagctag tttggggact tagagaaatg gcagctaaag
     9781 actcagaccg actttatact tattcgattc tattggtaat agcgagtggg ttcaacagta
     9841 ttttaaacaa gcaagcttat tctgatcgaa ttcgatttgt gcaaattctg agttttattt
     9901 atatcattat ttccgtttta tttttgcttt tactcagtat gatttatgat tttggaaaat
     9961 gctggtatcc ttctcgtgaa aatccatttt tcacttcggg taggcttatg attggaatga
    10021 tgattccttt tttaattcta tttgttgatg gaattagaat ttttatcctt tttattgaga
    10081 atctcttaaa ttggatttta tcatttatta ataaaagatt ttccgattca aaaacaaaaa
    10141 ttcaactttc tacaacgaaa gtattgatag ggtttatttt gttaatttgt tttttgatca
    10201 gtcagtatga aactaaatcc ttatggatgg taatgcagag taaatacaat tggttctttc
    10261 tttaaaaaaa aagattgtgt aaaaattttg gaatagaacc tttcttattc taaaacctaa
    10321 tttttagcac aatccagttg caataaggga gaattacatg aatgattatt gcgttctttc
    10381 ttttattttg accataattt ttacttcttt cccttttcgt aaaattgtaa aggacagctt
    10441 gtcgccaatt ttacttttac ggacaaagtt caccacatct tccggagatt tcattttttc
    10501 atcgttaatc tttgtgatta catcttgtaa ttcgagtccc gcttctgctg caggtgagtc
    10561 tccatagatt tgtttgatca aagctccttc agtagaggat aattttaact cgtcttttgt
    10621 ttcatcatct attgcatcta acgctatgcc aacccaaggt cttttaattt taccattagt
    10681 cttgagttct tccacgatac gttttgcttc attgattgga attgcaaatc cgataccaat
    10741 agacccacca cttggagaaa ctatcatacg attaatacca atgactcttc catagatgtc
    10801 gagtagtgga cctccactgt ttccttggtt gatggctgca tcagtttgaa tataacttac
    10861 tcctgaggaa tctatacctc cacgagcgat cccacttaca acgcccacag taaaggaatg
    10921 ctcataccca agaggagctc caattgcgat agcccagtcc cctaccttaa ctttggaaga
    10981 atcccctaga tcaataggcg atagatcctt cgcacctttg attctcagta gtgcaatgtc
    11041 cgaaacggga tctgaaccaa tgatctctgc ttcatatgat tttttatttc ttagttttac
    11101 atgcagttta tctgtttcgt ggataacatg ttcgtttgta agaacataac cttctttatt
    11161 tagaataatt cccgacccta gaccattttg tttctgtttt tgtgtttttc ctttgcctct
    11221 tggaccaaag aaaaatggct cgccagaaaa tggatccata aaagggttca tttgtatatt
    11281 gacggttttt tcagtcgcga tagacactac actgtccgct gtgtgctcaa aaacttcgtt
    11341 gaatgctgat tgcattccta tagcttgtga taatgcaggg gaaggattta cctctgtttt
    11401 tgcgttcaga gctaaactac ttttatttgc atctagacag taaaacagag gtgataataa
    11461 agtccctata aataaaattg agattggaat gatgaatttt ctattttttt tattcaaagg
    11521 ttttgcctcc ttagttatat ctattagaaa tcctttggct ttacaaagtt ctacattgaa
    11581 aaattttttt tacatatctt taccgaaatc tattttaatt actcttcttc tgcttagttt
    11641 cttctttgaa gtcaagggga acgaccttat atctttgaat gaaaaaaatc ttttagaagt
    11701 catgtctcat gtagactact tagaagataa aagcaattct ttggatttaa attccgtaaa
    11761 aagcctaaat ttcaaaaaaa attattctgg ctcgatcaac tttggacttt caaattctac
    11821 ctattggttt cgtttaaaaa ttaaaaatcc aacttcctct ccgatcgaaa attggttttt
    11881 ttttatcgaa tacccacagt tagacgaagt tgttttttat attccaatga agaatgatag
    11941 ttattttgag aagtcgatag gtgatatcta tccatttcaa gacagagaca tgaaaatcag
    12001 atttttaaat cttaggcttc cgaacggatt taaagaggga gaggacgttt ttataaaagt
    12061 aaaatctgta ccagctatta caattccttt gtttgtgggc actgaaattg cctttcgaaa
    12121 caaagatcat gtgcggcaaa tgtctattgg ttcctatatt ggaattcttt ttataatggc
    12181 tttgtataat ttatttcttt ttataatcct tagggaagct gcttatattt actatgttct
    12241 atttgttttg tctgtttgtt tgtatgtgat atccttttta ggtttaggtt atgaatttat
    12301 atggtcgaat tatccttacc tacaaagtca tatgatttta atttcatcat gcgttatgtt
    12361 atttaacctg tgtcttttta cgatttcttt tttcgataca aaaacttatg cacctaaaat
    12421 tcatatttat ctaatctaca gtttggttat tttccctctc acgattattt tcattttaat
    12481 ttcaaattca atattaaaag tatatcttac ttcaactctt gggattattt actcttttct
    12541 tttaccttta attggttttt atgtttggaa aaaaggaaat cgttttgcta gagtctatct
    12601 gtttgcttgg attccatttt ttatggcagc aaatctattt atccttaaaa catttggaat
    12661 cattgataaa tatgcattag agataaacta tttagtccag tttgccaata tttttgaagc
    12721 ggtagttttt tcttttgctc tagcagacag ggtaagagtt ttacaaaagg aaaatgaaat
    12781 cgctcaatta gaaatgttag atacaatgaa aaaagacatt gatagttttc gtctcttaca
    12841 agctgcaaat aagttagcca ataccgatag tctcactgga ttacacaatc gaaggtattt
    12901 ttatacgtta gtagaaacat tatggagaaa acctgatcct ctaattccag atatgtcaat
    12961 gctagtaatc gacgtagatc atttcaagaa ggtgaatgat acatatggac atgacgttgg
    13021 ggatcaggtc ttaaagcagg tagcagaaat tttatctaaa tcaattcgaa atatggatat
    13081 agtgggaaga tatgggggag aagaattctt ggttttcttg ccaaagacta acatacaaga
    13141 agcgatcgag cttgcagaaa aaattcgtgt atccatccaa gaacaaaaga ttctcctctc
    13201 cgaaaatcaa atcctccaaa ttacggcaag tataggagta gcctcaagag tagaaaaaga
    13261 aatggaaatc gatcagcttg ttaaaagtgc agacacaatg ctttacaagg caaaagagtc
    13321 gggacgaaat agagtcgttg gatttagtta gaatttgtag attcaattcc aatgatagta
    13381 atatcatctt gtatctttgt tgtgcctaag aattctgtta aatccgcgat tatcttttga
    13441 atggaatctt ttagaggtaa atcacgagta tcctttatca gagaatacag cctttcttcc
    13501 ccaaattctt ccaaactcga attgaattgc tcataaattc catctgaaaa aagataaagt
    13561 ctatctcctt tatgaaattc tttttctaaa tcattgtatt tattattctt tgtaattcca
    13621 accattcgtc ctgttttttc taagagttct attttaccat ttgataacaa aatgcaagaa
    13681 ggatgaccgg ctgacgcata ttgaatctta ctatttttgg gatcaatatc taaaaagata
    13741 caagtgagaa gtgaatttaa agattggtat ttttctaaga actcattatt aaaaatatct
    13801 aaagcatgag aaggagcaag gtcgaaattt tttatattat cataaattcc ttttactgcc
    13861 atggtaatta acgctgcctg aattccatga cctgtaacat ccgcgagaaa tactctatat
    13921 ttatctttca caggagaaga aattccatag aagtctcctc ccacctcaac catcgcagaa
    13981 taatagggaa caatttctaa atctttgatt tcagaataat caatgactaa agaattattc
    14041 tgaattcttt tcgcatagaa taaatcttgt tgaatggatt ttagagcgag atttaattct
    14101 tttgttctct catttacttt tgcttctagt tgactcgaaa gaatttgtaa ttcttccgtc
    14161 agtgcctctg ataatacaaa tgcatttgca aatcttctag acaatacaat tgcttgtgat
    14221 accacaaaaa acaaaagacc ataacttgct aaaaatggac tatatacaac tcctctagtc
    14281 ctaagaatat catgaatgat agttacaaaa aaaatcagaa gaccggctaa gaatgatttt
    14341 gcgccaacat gtttatttcg aatcgcataa aatataattt taagtgtata tactatccca
    14401 actaaaatgg tcaactgaac aaaaataaga gttcgagtgt aaatcgacat agtgcttaca
    14461 agaactaaaa ttgaacagag tataaaaata gttttaaata ctttataaat tctaggattt
    14521 gtatcttctg ggaaaataga atgaatgaat tgtaaaaaaa ctaacgatcc gtaataaaaa
    14581 gaaaagtatt ctattttatg aacgatcata aaaggaatat cagaccaaat ttcgtagatc
    14641 aatctctcct taattgcaat cgttcggagg actactaaaa tacaaaatag accaaagtaa
    14701 aaaggagaaa tatcttttct tctgttaaaa aataaaccga tatggtaaag acccattata
    14761 agaagggcac taattacgat taaatcgatt gctatactat gaaattctct ttcgtgaatt
    14821 gtttttgctt ctcctaaaac aacgctttgc caaactcccg ctttataata atgaaaattg
    14881 gagttgtgaa taacaatgat tagctctgta tcagttgttt taattgcagg aaaaataata
    14941 tattgttgtc tcacaaacat ttcatcggca gatttcccta ccttaccgtt tgacgcacgc
    15001 agagatccat ttacaaatag actataagcc attccagtat cactcataga aaaataaaga
    15061 tttggatgct tttctgggag gattactttt aaaacataac tagcatatcc gtaagcagga
    15121 taaaaatttc cgcttggatc tttttggttt tgccattggg aaggaacttg aatataggaa
    15181 ttaactttat tttcccagtc agacgttttg ggatcaataa actcattcca aaaaaaagcc
    15241 cattccccat cgagttgaat agaatcagaa aagggagaag aaaaatcgat ttctcttaga
    15301 tcgatcactc ctttgattgc tttgggaatt tgcttttcaa ttttcggttt gatgcaagaa
    15361 gaaaaaaaaa tacttaaaaa agaaggcaat ataaatgcta gaatgatttt tttagaaaac
    15421 atcgtctaat ctctattatc cgcggatttt aattaaaaaa ggatttgtaa ttgcattccg
    15481 aataagacta agattaccaa aagagatagt aaggtcaact ataaacaagg aaaaatagtg
    15541 tatcaaatta gtttctggat aaggaatcga agtctaaatt gattgcagaa aatttaaaag
    15601 aatcaaaact actcaagata gtttttaaat attatgaaag aaatagggaa gattagcttt
    15661 agaattgtta taataacttt tttaatagaa ttagctttct cttgcactgt gcgtcctaag
    15721 actaagatac tcggcttatt tagtttagtg aatcgggagg taactatttc ctacaattta
    15781 ggagggactg tgtcaggttt acaaggaact cttattttac aaaataatat gatggataga
    15841 ataaccattg aggcaaatgg aaattttaca tttacaaatc tatatcctac gaattcaaat
    15901 tattcggtaa ctgtcttttc tcaacctagc tcccagactt gctcgataac aaatggctca
    15961 ggaactattt tgagttcttc catatcaaat atatctgttc gttgtggtaa tacaatcagt
    16021 agtttatctt ctctaacaat tagctcaggg actattactc ccagtttttc ctcttctctt
    16081 tattcttatt ctttctctgt tttaaactca gctagttcta taaccatcac accgactgca
    16141 acggatgcaa gcgcaaccat tactgtaaat gggagcagtg ttttatctgg atcttcatcg
    16201 agtcctatta gcttgagtgt aggatcaaat acaatatcga ttgtagtaaa agcttctgat
    16261 ggaattacta catccactta tactcttaca gtcattagat caaccctaaa cgcctatcgt
    16321 atttttataa ctgcatcgac ctatgatgga aatttaattg gaacaagttc ttcaggctat
    16381 cttggggcag attctaagtg taattctgat gcaaataaac ctacagatgg aagtatttat
    16441 aaggcggtaa ttacagacgt agtaaatcga agtgcctgtt ctattaatat caattgcacg
    16501 aatccatctg aaaatttgga ttgggcattg cggactaata ctagttatgc gagaacagat
    16561 ggaaccgctt tattcactac aaacgcagca ggtatttttg tttttgggac tctcacaaat
    16621 tcgtttggag gagggcaata ctggactgga attagcacgg tcagttcatg gagaccatct
    16681 acaggtggga ataattgttc tgaatggtca tcgaatagcg gtgcgttaaa tggtaactat
    16741 ggggatggat ctgtcactaa ttatacttcg atcgctatga catcaggggc gtgtaataat
    16801 ttaaaatctt tgctctgtgc agaacaataa agaatcaaat caaaaagata aattctaatt
    16861 ggtgttgttt agaattagaa ctgccaaata atatcaggtt ttaaaataaa attactttac
    16921 aagaatagga aaaaagatta tgttatatct cattttttaa agtcattagt attagggttt
    16981 gctgtctagc aaaatagagg gtttttattt catgttaaaa aacaaagtat acttaattgt
    17041 ttttagtctg gtggtgggcg tattatttta ttttctatat aattcagata ttcaaaaaaa
    17101 aactcattcg cataacgata gtcctaatct ttccggagaa aaaataaaat ttttagttac
    17161 aataactcag gcaagagcag caagagcatt ggcagattgg tatacgaagg aaacgaaagc
    17221 agtggttcaa attacagaaa ttggatacga tgaaacctat agaaaaactc tagaagatat
    17281 tcgttcctcc gatcctcaat atgatatttt tatcatttgg tatccagagc taggagactt
    17341 gataagaaat aaagcgttat tagatctcac tgactggatt gaggaaaata aggaaaccat
    17401 tcaaccccaa gattttattc ctaggattta tgatgcgtat acattacaga agggaagacg
    17461 ttgggcttta ccatttgatg gggatacaca tgttttattt tatcgcaaat ctatactttc
    17521 aaaacataac ctgaatcctc ccgagacttg ggacgattat ttgaacgtta gtcgaaccat
    17581 aacagaaaaa gaaaaagtaa atggaattta tggaacagcg atcatgtcct ataaagcgcc
    17641 tatattaatt ttatccagtt tcttaaatcg agtaggagga tatggcggac aacttctaac
    17701 aaaagatgga agaccttctc tagatacacc agaagtaaaa atggctctag agagtttgtt
    17761 ggagcaagct gagtatgcac ttccctcacc gctagagact agctttgaag tttcaagaga
    17821 ttcatttttg tcgggtcggg ttgcaatggt ggaacaatgg acggatattg gagtaatggc
    17881 agaagataat agcgaatcta caattagagg agattggggg gcaatccaga tgccaaaggg
    17941 gagtggaaaa aatgcccaac atgcaccagc tctaaatgcc ggatttgctc ttgctatttc
    18001 ttccaaagct ccaaatttag aagtagcaaa agatttttta ttatttgccg ttcgccccga
    18061 tataatggca aagtataacg ttttaaatgg aggaacagat cctactcgca tttccatatt
    18121 gaaatcagat gattatagaa agtttgcgcc agaggtaagt gctgccgctg aagcagctct
    18181 ttttaatgca acaccctggc ccaatattcc aggaagtaga aaacttatgc agatactttc
    18241 agataacatc gttcttgctt taagaaaaaa aatttctgta gagaatgcaa tctctgatac
    18301 acaaaaacgt tggactgaaa ttatcgattc tactaattag taaacatgca tcgattctta
    18361 ttttggcaga agcttctttt attgatgggt gcttctatgt tagccctaac cttaggatta
    18421 tcctttgcaa cttggtattc tttcgttcag gcacaaaaag ggatcggcat tgaaggttat
    18481 aatgttttat ccaatcaaac agagcaattt ttaaaaaatc aagcacaaga gagaagtaga
    18541 agtcttgatt tacaatttgc gcaagctcaa tctgttgtat cgtatggagc aagtgaaatt
    18601 gcactcattg tttctgaaaa acaaatagat aaaaaaaaga ttttgacagc tctaaagaat
    18661 ctttttcact ctcttggaaa ttcgaaagct aaggtatttt tttacagtaa aaaatctggt
    18721 ctcataactt atcaagaaaa caaacaaaat aaattagaag aagaacatgt agaatttcat
    18781 tcgccaaact tttttccggg gattcataac tttgcaaaaa aaagtgaatc ggttctttgg
    18841 agtaagccac atccaagttt atatgatgaa tatgaatggg ttgtagatat tattgctccc
    18901 ttaaatagat taaatcccta tgaaactttt atcggtatct ctttttctct tactgacttg
    18961 attactcaat tcaatcagct tcatcccact agaggtagtt attgtttttt aatggatgag
    19021 aatcaaaagc tagttgccgc accacctcat gctagggtag agctcgcgcc taatgataat
    19081 tctttgaaag aggaacaaat tcatttgaat ggttatgagg ataaagtttt agagcaggct
    19141 ttaaaagaaa tggcattagg aagatctgct cttgtgaaag tttccataaa aggcaatgag
    19201 aaatatttgg catatcatcc gcttgaagtg atgaattgga aagttggtat tgttgttcca
    19261 gtttctattg caacctctgc atctattcaa ttacaaagag tcgttgaatc tgagatgaaa
    19321 aagtcttttt acagaatttt tattgctgca ttttttattt ttggaatttc cttgttaggt
    19381 ggaagtttct tggttcgaca tttactagtt ccacttgagc aggtatcact tgcatccaaa
    19441 agtatgctag atggggactt caaacaaaga gttatcgtaa aaagcacgga tgagattagt
    19501 aatcttgcaa gagtattcaa tcaaatggca gatcatatac aggtattaat aagccactta
    19561 gagcaaagag caaatgaact ggaagagtta aatcaatctc tggaagcaaa ggtagtggaa
    19621 agaacaagta aattagaaga aagaacaatt caattagata aagccaataa acaattacaa
    19681 aaagaaatga gagacagaga agaagcacag gaagctctta ccattgccaa tagca
//
LOCUS       DAOQOS010000103        19706 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_1374_length_19706_cov_5.466134, whole genome shotgun sequence.
ACCESSION   DAOQOS010000103 DAOQOS010000000
VERSION     DAOQOS010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 19706)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19706)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19706
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1374_length_19706_cov_5.466134"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..1384)
                     /locus_tag="PLF66_19005"
                     /pseudo
     CDS             complement(1..1384)
                     /locus_tag="PLF66_19005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004754730.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
     gene            complement(1471..2541)
                     /locus_tag="PLF66_19010"
     CDS             complement(1471..2541)
                     /locus_tag="PLF66_19010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016545264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HMZ66188.1"
                     /translation="MAGKITHIEVLAQATKHLDHGTSEQRNIAHLLLDKSNKNYANLG
                     TIAPDIFYYYHILSPSKITSKAQIWGDLHHHEKVAELVLNFLDIIIETEEGIYRDRMI
                     AFVLGYICHCAVDIVTHPYIFFISGDYYNKDPEIASLAQYNHMRVEFALDSYLLDHRW
                     GMSPKDYDFTHYIDVRRRGVSGLKKIDPMIWVFWIEALRLTFPEEFKKNYIGSMRKII
                     PGDIINDSYLGYIRFNEVLDSRSNGMRNFLKMVDFISFTKKKASTLLMPLKESIDTRV
                     LNSDEKEWFYPADASRKKNHSFITLVNQAANAARDAMTNAWEYLKGNIKREAILKEYG
                     GYNLDTGLRYQGISEMKEFAPL"
     gene            complement(2615..3388)
                     /locus_tag="PLF66_19015"
     CDS             complement(2615..3388)
                     /locus_tag="PLF66_19015"
                     /inference="COORDINATES: protein motif:HMM:NF014590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HMZ66189.1"
                     /translation="MSLIFENDLGMVSWFRLFFCILFGSIFFYVEVTKKYQLSYSKFN
                     ESGNLDSKKGMLFIYFLPLLTYIYFYWHSNFTPTFYHQMVLIAVSIHFAKRCLESLFL
                     HRYSGKISIVTTFFIAWAYSSIAYTIHESVNLFTKIEKVDSEMSVSIWIGFLIFLLGQ
                     SSNLYHHILLTKLRASGSKEYKIPEGGLFKYVNCPHYLSEIIGWIGIAIMSRYLIVFG
                     LTYIMSAYLVARSINTTKWYQEKIPNYPRERKSILPFVL"
     gene            3530..4591
                     /locus_tag="PLF66_19020"
     CDS             3530..4591
                     /locus_tag="PLF66_19020"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HMZ66190.1"
                     /translation="MIKTTQTIKNQTITQKPFRHSSQFSLSELKKTIPTELFESSLLK
                     SIFYFGVDVFVISGLFYLAATLDSPFFYPLYWFLQGTFFWALFVVGHDCGHGSFSKYK
                     WINSLFGHLAHTPLLVPYHGWRISHRTHHNNTGNMDKDESWYPLSESDYKNLPWSSYV
                     LRYKLFLLVFPLYLFVRSPNRDGSHFLPSSDLFSPSEKWQVLTSTALWIMMISLLGYL
                     GYTFGFVKLLNLYILPYFVFVVWLSLVTFLHHTDTNLPWYRSSNWNFVKGALSTIDRS
                     YGIFEPIHHNIGTHVVHHIFSKIPHYNLVKANQHLKEALGEHYLSSNESIWKAYVRAY
                     QTCHYVSDEGDTVYYIRDK"
     gene            complement(4747..5262)
                     /locus_tag="PLF66_19025"
     CDS             complement(4747..5262)
                     /locus_tag="PLF66_19025"
                     /inference="COORDINATES: protein motif:HMM:NF025700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4396 domain-containing protein"
                     /protein_id="HMZ66191.1"
                     /translation="MNTLQHHKHHGHHSEEMHHTAEDLHHPSHTEKDSVFKIAMQATL
                     HCLLGCGIGEIVGIILAASLGLGMWESMIVGILLGFVFGFTLGMIPLVKAGFSFIESF
                     KTILVSEGLSILVMEGAEAITQVYIPGVMEAGLSESIFWYGMGISLFAGFIAALPVNL
                     LLISRGIRHHH"
     gene            complement(5314..5736)
                     /locus_tag="PLF66_19030"
     CDS             complement(5314..5736)
                     /locus_tag="PLF66_19030"
                     /inference="COORDINATES: protein motif:HMM:NF024864.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HMZ66192.1"
                     /translation="MKLKNLTILFILTTGLFASCAKEQKESNVDYSIITKEMSFSPNE
                     LHLKKGQTYRIRLHNEGSVLHDWNVEDIKANVHHSSDSGSIHHHGHNKKMPLHLAVLP
                     ARSNEIEFKAEEAGVFTFFCSVPGHKESGMVGKILVHD"
     gene            5821..6375
                     /locus_tag="PLF66_19035"
     CDS             5821..6375
                     /locus_tag="PLF66_19035"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HMZ66193.1"
                     /translation="MHTHADHLTNELLKYRKELIGFIIKRTQDPDLAEDIFQDSLLKL
                     VRKSPDIEDTQSLLAWFYTLLRNAIIDTYRKKNTDAKKLESIKKSFQTEINSTDKNDL
                     CECFKEILPILNPDYKEIIEADLREEDTDTTAKRLGIEKNNLKVKKFRARKQLKERLE
                     QTCRMCAKHGCLDCTCKKDSSHSN"
     gene            complement(6396..8903)
                     /locus_tag="PLF66_19040"
     CDS             complement(6396..8903)
                     /locus_tag="PLF66_19040"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HMZ66194.1"
                     /translation="MKKLALFPFLILIYSSNLFAQKKEIDNTNLEKIKKEREELEKEK
                     ENFKKEKSEFEAEKKKFLEESQESAKSEESNESKKLTDEDFFRLEENIVVTAAKKEQK
                     VSDAPAAVYVITEKQIRERGYRVLTDALIDQPGFNFIRAYGVWSDLMFQRGMINNSNQ
                     RSIVYVDGIPENILSENSILAGTLRFPLQNVERIEIINGPASSLYGANAFNGVINIIT
                     KTGKDRPGNEVTAMYGAWESNGRNPGYSTSITSRNSIGKGEQAMHYSASAYLYKTEGP
                     YFGDQKRLDKPNVNPNDAAYYYESKACGGQCQPDSKSVGYYWSNRYNIADVDTYNITG
                     KLAYKGFRFESVNHQYIAGTGNFQNGTRRLDYHDKGLDSDNFDSRNNFRRLGILNGQI
                     TANGQPGAYWNFRSNSVYMAYLWTINKKMSLDSEVISRSTVLLNSSVEGIYRNPGPDA
                     FYKPDDVTLNPRTRPDYGNELREKFTYEHSSRLSLSIGTEMSYTVVPRGYNSYQSFKY
                     SNYGLYGQAIYSPVSFVSFTLGYRFDENTIYGKTYNPRLGTIIKPFENFTIKILYGTG
                     FRPPSGWELFSATSARKENTRLSPERMKSYELGFEYRVLNKYFFTLNGYYNRVHDIIL
                     EVKTDDASVDPVTKLRPANGTWSQNQNAGDSKIYGFELSNETAILKNLRIHFNYAHTK
                     GDFVNLSRIITSSPAVEGRPGDDYALDLVNAKTNRSFVPESGRIPLYPENILNSGITW
                     NIIQNLSFYMGTNWLDVRRNVATNPIKTTHQIFFVKTNIRWEDAFWEGLFIQLQINNL
                     LNKQYFDPGARTAEGLGTPTLMPLEGRNYWFTLGYKF"
     gene            complement(9055..10857)
                     /locus_tag="PLF66_19045"
     CDS             complement(9055..10857)
                     /locus_tag="PLF66_19045"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HMZ66195.1"
                     /translation="MNRKEFLKKSLQVGATGVGLGLSGGIGYSFFANSEYKKDFPHLP
                     ENHVSLSLNGKTVCILGAGLAGLQAACELSDRGFKVVLVEKTSYAGGKLKTWKDKHFA
                     KKHFGEKGYSREHGLHAIWGFYKNLREFLGRHKIEINKLGKDDSFYYFISKRGVQSKI
                     QNTTWPIPFDRIEMYDNGIYVPSREDIRVPASNLFETLRTSMKMWGFDFSNKEDRLYL
                     DSITFYDWAVKNGMSVEYIKHFFDALAEMGFFMTTKECSALAIVNFMRLGNLPKDSKV
                     DLYKWPPDETFIQPMINHILSKGGEIYFNHEVTSIIRENGKIVSIKTNENFPKSRTKR
                     CRVCGNIMGDGDYEHCPFCGAHHSELEILKDTTPLEVKADYFIAAMDIPGAKKFILGT
                     GLDKEGDYFKKATLLTNAHILCVNLFYENSDAWKKRFPEGKYSAYDFMPTGYENLGFT
                     TNWSSKQIPELREKNIDLIEVQVSKWQELLKYSHREIANIVHTELKQILPDLKEFSEF
                     YINHWDTYTGFRPGDEANRPEIQSPIENLLFIGDWVFVDQHSVFMEKTNVMAKTVTNL
                     LLDKIGQKEGKITILKSGTPDWPTDLLKYFTSIR"
     gene            complement(10854..12056)
                     /locus_tag="PLF66_19050"
     CDS             complement(10854..12056)
                     /locus_tag="PLF66_19050"
                     /inference="COORDINATES: protein motif:HMM:NF024779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4105 domain-containing protein"
                     /protein_id="HMZ66196.1"
                     /translation="MFRYFLIFLFLVTNSIFSQGDYKINIENKDKFVSFINSLTPKDL
                     EDLKIQLITVDTSPTVTTAFGHSALRVFLGKQFEDKDFYLDFGEYDESAGFIWRFLKG
                     EAKFYITVKTMGLAYMQWDTTGRGMYASEFKLDANQKKLFLKNILSFIEKYKDGYEYN
                     NFTGNCVTYIRDLIGETYGTPLKLETESNKRTWRNRVIPYSNKIFWLRMEEKLLLDHD
                     TDKERNPTELIYLPYDLLFAVEDAKLVKDKELLHRNFWQMPPDSFDFLGNLMFIILVF
                     IIVGQIPVYFKSMIDRKGEILFGIISGFGGLFALVVRLFTSFPFMDETIMIFVFSPID
                     FFLLAKKKPSFKIHMGIILLRLSMILLATVFRFTIYKQTIDTALFFTVIFYVLYAINT
                     YRARAIAQ"
     gene            complement(12057..13142)
                     /locus_tag="PLF66_19055"
     CDS             complement(12057..13142)
                     /locus_tag="PLF66_19055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66197.1"
                     /translation="MNRLYTILILITILSCTADRKLNDPTEQLKGFRCEGNDYYVDYI
                     CEDEKEYLDWAASYPKYRKLSDLTREVLFNIMKETNDIDKAAAVFYHRAITEPTNRKF
                     IEYIYTKQNEFMRKVPDYSSKKMLFAIAPGMFYRDNKEVDADGKLIRAIVKQLGLGEA
                     VIPTDQTGTVDGNGKIICDFIKSQTEYKGIIIGSASKGSGDFKKALELCGKEPYFSKV
                     VGWFNFGGINKGSLVVNGMMDHWLYRWEGKFYFWVMGYNWEGLTSMRGGENAPLSKEL
                     EIPKNMTVVNIVGVPTARLVTERGRPFYNYLAQFGPNEGLNLLSDSHIEGVPLFPSWR
                     NDHYFRVPMPEERIKAFIAYIVEKQGK"
     gene            complement(13238..13651)
                     /locus_tag="PLF66_19060"
     CDS             complement(13238..13651)
                     /locus_tag="PLF66_19060"
                     /inference="COORDINATES: protein motif:HMM:NF024546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRL domain-containing protein"
                     /protein_id="HMZ66198.1"
                     /translation="MNKNFKNSLGVLTALAIAAAIACSGGSGSGAGPSIQPASSGSIM
                     DNVKSNQNCTWGGDITNAGEAGCVWGKDGGKPVGTKTGKACTNAILGLIRSGDMSIAA
                     AAKQGGITKVQSVDASINSLLGSVLVESCILVNGT"
     gene            complement(13767..14807)
                     /locus_tag="PLF66_19065"
     CDS             complement(13767..14807)
                     /locus_tag="PLF66_19065"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HMZ66199.1"
                     /translation="MQNLGKSLLFIGLLVSILNCAGNKKLFEAARNGDEKKISQYINE
                     KKDVNVKDKDFGGTLLMYSAGFGYTNIVKMLLDAGADVNATNNLNGTALMNACNYGYD
                     EIVKLLLEKGADVNTVDTLQRTALMFAIARYRLEITKELLKSKANVNVRNKEGVTPLM
                     LAVNFQFTEALPILLKEKADINATDLFGETALIKAVYKNDIDSIKFLIQNRADVNSKA
                     NDGKTALIIAAKKGQKDMVSALIKAKADLNLADSSGYTPLIWAVNMGEMEIIRQLLSA
                     GANPNMQDERGQTALMKSVLQGNLEMTALLLSKKHDLTLKDKSGQTALALAIENSQGD
                     IVNQLKKAGAKN"
     gene            complement(14825..15835)
                     /locus_tag="PLF66_19070"
     CDS             complement(14825..15835)
                     /locus_tag="PLF66_19070"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HMZ66200.1"
                     /translation="MFPVNIDWYFIHFRGYEGHWAIGILIFGYFYQRYRSLKAGYDIE
                     WFNSAWAAAVGSGIIGARLFHFAFWETTNFLNNPMIIFTSTGGFAILGATIGTAFGGW
                     AYCKYTKKDFLSWCDSLMLPLTVGLALSRMSCFLNGDAYGLPTASILGVTFSENSDAW
                     MAEWRHLHQFYANSEDPLAIISQMFSKYVNLADMPIPDSMSHLKDLGFKNLAELTRYY
                     PPSATGDYKQELLKLGLIPFPVVYPKVHPTQLYEMALMFIVLFIMVRIDKKEWAKRKL
                     FFIFWALYGTNRFFVEFFRGDRNLIFGSLTYAQVITLLIIVSSIIALIIQFSTNHKQM
                     ES"
     gene            complement(15844..16881)
                     /gene="fbp"
                     /locus_tag="PLF66_19075"
     CDS             complement(15844..16881)
                     /gene="fbp"
                     /locus_tag="PLF66_19075"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020768169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HMZ66201.1"
                     /translation="MKSATEMISLSQYLIEEQLKLPYATGDFTALMQHLVYAAKIVSR
                     EVRKAGLTDIVLGATSETNVQGETQMKLDRYADVLFTKALSICGHLCVLASEENESII
                     EIPSGYKMGKYTFAFDPLDGSSNIDSNVSIGTIFSIHLRKSKHGGPGEESDLLQRGAL
                     QRCAGYILYGSSTMMVLSMGNGVVGFTLDPSCGEFLLSHENIKIPETGSIYSINEGNY
                     DFWSDAVKSYIRTIKNIENNNKPKTLRYIGSLVADFHRNLLKGGIFLYPEDTKSKSYP
                     LGKLRLLYEASPMAFLAEQAGGMAVTVSGERILDMEPTSLHQRTTLVTGSKKEVEFFL
                     ECTKKFRQSNN"
     gene            17335..19380
                     /locus_tag="PLF66_19080"
     CDS             17335..19380
                     /locus_tag="PLF66_19080"
                     /inference="COORDINATES: protein motif:HMM:NF021942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HMZ66202.1"
                     /translation="MTLTTIGIATQLSSDEGMWTLDNLPTKQLEEKYNFKLTDKWLKN
                     AQLASVRFNDGGSGAFISAKGLVITNHHVAMGQLQKMSSKKTDYVKDGFFAQKESEEI
                     KCPDLELNVLISMEDVTKQVVTASEKGDTDKAKNEKRKEEIAKIEKESNEKTKLRSDV
                     VELYDGGEYWLYRYKKYTDVRLVMAPELQAASFGGDPDNFNYPRFALDMAFFRVYENN
                     KPIESKNYFKWSKEGAKEDELVFVFGHPGSTERQKTMSQIHYQKEYAFPEYVNLLKRK
                     LKLYRDYSEKGKEEKRRAKDYILGMENSLKAIEGELSGLKDKKILDSMEEKENKLKSL
                     VSQDKELQKEYGEIWNRIDSVQKKLIAKHKEFYYRSVSNTRLVNIALKLVRYATEIEK
                     PNEKRYEEYRDSNLESLKFRLLSPAPIYTDMEEMSLAHTLQLAVEKLGKDDEFVKIAI
                     TSDPKEFAKNLITKTKLGDVAYRKELLKEGKAGIEKSKDPLIEWIRKVDPILRKDRDW
                     FDDEIESVQAIEGHRLSALKFKVFGKSMYPDATFTLRMSYGQVKGFTDDTTIEVPFKT
                     TFYGLFDRAESFNHREPFKLSTKILENKSSIQMNKPLNFITTNDIVGGNSGSPVVNRK
                     GEYVGLIFDGNSYSHILSYAYTDNKARAVSVHSEGILEALRSIYKMENLVKEITAAP"
BASE COUNT         6424 a         3647 c         3206 g         6429 t
ORIGIN      
        1 cataaacgag tggtttaaaa gatgatcccg gttgacgata ggcttgaatc ggtctaagct
       61 gttgattgtc ggagcgaaac ccagatcctc caaccatcgc agttatataa ccagtttcag
      121 gacgcatact aatgatccca ccttctactg gtagtaaatg atcctgtgta gtttgtttcg
      181 cataattttc gtcgaaaata cttcctacac tatcagagcc cgtcagtaaa ttgagagctg
      241 cataattatc tcttagttct tcttggtata cacttctaaa tgttctctcc attctagaaa
      301 ttttaaactt aaaatcacta atatcatgcg tgagtgcaat tagattgtaa acatcaccaa
      361 attgatcatc aaaagcatca atatttttaa atgctctttg atttgaaatc tttgtttgat
      421 tgataagccc tgcagacaat acagcttctg cttcggtttg gtgctgaata ttcatggtag
      481 agtaaacttt taatccaccg ctatataaat cctcattact tacatgtttc ttgagaagtc
      541 ttcgaatgta ttcagtaaaa tatggaaatc gattgagtcg atccgagtaa gcagagtcgt
      601 taggcgatcg attaaggctt gcatagtatt cagagaattt tttgtattcg gattctgcct
      661 ttgcaatgtc catcttacca ttttccacaa gcttcataaa aatgactctg actttattgg
      721 cagataccat cggatttact aaaggactaa attcttttgg tcttgttgta agacttgcaa
      781 ggagtgccgc ttctccccaa ccgagagagt tgatatcttt tctaaaataa aaacgggctg
      841 ccgctccaac tccgagcgtt ccatgcccga gtggaatttc atttaaatac atttctagaa
      901 ttgtattctt gtcataataa agctccatta aaaaagcgag ccatgcttca cgggcttttc
      961 gaataaagga tctgtctgta tttaaaaact ttagacgtgc tacttgttgt gtgatcgtag
     1021 aagccccttc tttgattttt ccagcaacaa tattaactgc aaaggctctt acgatccctc
     1081 ttagatctaa tccatggtgt tcataaaaat tactgtcttc cgttgagaga aagcattgta
     1141 aaactttatt tcttttgtct ggagaatctg tttcttcttt aaagctaggt agatttaata
     1201 cgatacgcga aaactgatag aactctgcaa ttgcctcata ttctcctttc gtattcacac
     1261 catagagagt tgagggaatt tcataatcca ttgctttggg aattaatttc caatcatcca
     1321 aagaaccaac gattagaaaa acattgagaa ctccgaggaa aattaaaaca aaaattaatt
     1381 ttaagaaagc ataacttcca gataaaacag aattcactag agaggcaaac ccaccaatga
     1441 taaaagtgct tagagaatta taatttcgat tcataaaggt gcgaactctt tcatttcaga
     1501 tattccctgg tagcgaagac cagtgtctag gttataacca ccgtattctt tcaagattgc
     1561 ctctctttta atgtttcctt tgagatactc ccaagcattg gtcattgcat cccttgccgc
     1621 atttgcagct tggttgacca aggtgataaa ggaatgattt ttcttacgac ttgcatctgc
     1681 aggataaaac cattcttttt catcggagtt caaaaccctt gtgtcgatgg attcctttaa
     1741 tggcataagt aaagtggatg cttttttctt tgtaaatgag ataaagtcaa ccatttttaa
     1801 gaaatttcgc attccattgc ttctagaatc tagcacttca ttgaatcgaa tatatcctag
     1861 ataggaatca ttgatgatgt ctccaggaat aattttacgc atagaaccaa tgtagttttt
     1921 tttaaattct tctgggaaag taagacgaag cgcttcaatc caaaacaccc aaatcatcgg
     1981 gtcaattttt ttaagaccac tcactccacg tcgtctaacg tctatataat gagtgaaatc
     2041 ataatccttt ggactcattc cccatctatg gtcgagcaaa taagaatcga gtgcaaattc
     2101 tactcgcata tgattgtatt gtgctaagct tgcaatctct gggtctttgt tgtagtaatc
     2161 tccagaaata aaaaagatat aaggatgcgt tactatatct actgcacaat ggcaaatgta
     2221 tccaagcaca aatgcaatca tcctatctct gtaaatcccc tcttctgttt cgatgatgat
     2281 atccaagaaa tttaaaacaa gttctgcgac tttttcatgg tggtgtaaat ctccccaaat
     2341 ttgtgccttg ctagtaattt ttgaaggaga taaaatatga taataataaa aaatatctgg
     2401 tgcaatggtt cctagatttg cataattttt attggattta tccaaaagta aatgggcaat
     2461 atttctctgt tcacttgtgc catgatctaa gtgttttgta gcctgagcca atacttctat
     2521 gtgagttatt tttccagcca taaagagata caaatgccaa tgtctgactt tttacttatt
     2581 aagcaaatca aaaacaaatg ggattaggaa agattcataa cacaaaaggt aaaatagatt
     2641 ttctctctct aggataattt ggaatttttt cttgatacca tttagttgta ttgatactgc
     2701 gtgccactaa ataagcagac atgatataag ttaatccaaa tacaattaaa tatctactca
     2761 taatggcaat tccaatccaa cctatgattt cacttaaata gtgaggacaa tttacatatt
     2821 taaaaagtcc tccttctgga attttatatt ctttagagcc acttgctctg agtttagtaa
     2881 gtaaaatatg gtgatacaaa ttcgaggact gaccaagtaa aaaaatcaag aacccaatcc
     2941 atatagatac actcatttca gaatcaactt tttctatttt agtaaataaa tttactgact
     3001 catggattgt ataggcgatc gaagaataag cccaagcaat aaaaaaagta gttacgatcg
     3061 aaatctttcc tgaatatcga tgtaaaaaca aagattccaa acatcgcttt gcaaaatgaa
     3121 tcgaaactgc aattaaaacc atttgatgat aaaaggtggg agtaaaattg gaatgccaat
     3181 aaaaataaat ataagtgaga agaggcaaaa agtaaataaa gagcattcct tttttagaat
     3241 ctaaatttcc tgactcattg aatttactat aagaaagttg gtattttttg gttacctcaa
     3301 catagaagaa aatagatcca aacaatatac aaaaaaacaa acggaaccaa gacaccattc
     3361 ctaaatcatt ttcaaaaatt aaactcattc taaaaatatc tcctaaatat acagttttaa
     3421 aatagcattc ctacttaaag caggtttttt tagtttctta tttctaattg catttaatct
     3481 aacaacgttt tttatggatt cagttacttt atgaaatttt tgcctatcta tgataaaaac
     3541 aacacaaaca attaaaaatc aaactataac tcaaaaacca tttcgccact caagtcaatt
     3601 tagcctttct gaactaaaaa aaacaattcc aactgaatta tttgaaagtt ctctactaaa
     3661 atcgattttt tattttggtg tagatgtttt cgtaatttca ggacttttct atttagcggc
     3721 tactcttgat tctccttttt tctacccact ttattggttt ttacagggaa cttttttctg
     3781 ggcattattt gtagtaggac atgattgtgg gcatggatct ttttcaaaat acaaatggat
     3841 caattctctc tttggtcatt tggcacacac tcccctttta gttccctacc atggttggag
     3901 aattagtcac agaacacacc acaacaacac aggtaatatg gacaaagatg agtcttggta
     3961 tcctctatcc gaatctgatt ataaaaactt accttggtct tcttatgtat taagatataa
     4021 actatttctg ctggtatttc cactctatct atttgttcgt tctccaaacc gagatggatc
     4081 tcatttttta ccaagcagtg atttattttc tccttctgag aaatggcagg ttttaactag
     4141 cacagctttg tggataatga tgattagcct actaggctat ctaggatata catttggctt
     4201 tgtaaagctt ttgaatttat atatccttcc ttattttgta tttgttgttt ggctttctct
     4261 cgttacattt ttacaccata cagatacaaa tcttccttgg tatagatctt ccaattggaa
     4321 cttcgtaaaa ggagcacttt cgaccattga tcgatcctat ggaatctttg aaccaattca
     4381 tcataatatt ggaactcacg ttgttcatca tattttttcc aaaatcccac actacaacct
     4441 tgtaaaagca aatcaacatt taaaggaagc gttaggcgag cattacttga gttcgaatga
     4501 atccatttgg aaagcttatg taagagctta tcaaacttgc cattatgttt cggatgaagg
     4561 tgatactgtt tattatatta gagataaata aggattttct aaaagtttta ggaagtgaaa
     4621 tttattattc ttttccccct gccatgaata gctccaatcc tagatagaaa acaagttgaa
     4681 tttaaataaa aagttatgta aaaaaaattc ctaagaacca ccgacaggtg attcttaggt
     4741 tgtattttaa tgatggtgtc tgattccacg actaatgagg agtaagttga caggaagtgc
     4801 agcaataaat cctgcaaaaa gcgatatacc cataccatac caaaagattg attccgaaag
     4861 acctgcttcc atcactcccg gaatataaac ttgagtgatc gcttctgctc cttccatcac
     4921 taaaatactc aatccttctg atacaagaat ggttttgaat gactctataa aagaaaatcc
     4981 ggcttttaca agcggaatca taccgagtgt aaaaccaaaa acaaatccga gtagaattcc
     5041 gacaatcata ctctcccaca ttccgagtcc taaactagcc gcaagaatga tcccaacaat
     5101 ctctccaata ccacatccaa gtaaacaatg gagagttgcc tgcattgcta ttttaaaaac
     5161 agaatctttt tctgtatgag aaggatgatg tagatcttcc gccgtgtgat gcatttcttc
     5221 tgaatgatgc ccatggtgtt tgtgatgttg taaagtattc ataaaattct ccttatttaa
     5281 aattggtaac gtttctcttt tgttttggtt acactaatca tgaactaaaa tttttccaac
     5341 cataccggat tctttatgcc caggaacaga gcaaaagaaa gtaaatacac ctgcttcttc
     5401 tgctttaaac tctatctcgt tggatcttgc aggtaatacg gctagatgca gtggcatttt
     5461 tttgttgtgt ccgtggtggt ggatcgagcc agaatcagag ctgtgatgca catttgcttt
     5521 tatatcttct acattccaat cgtgtaaaac acttccttca ttgtgcaatc ggattcgata
     5581 ggtttgtcct tttttaagat gaagctcatt gggtgaaaaa ctcatctctt ttgttatgat
     5641 agaataatct acattggatt ctttctgttc ttttgcacaa gaggcaaaaa ggcctgtagt
     5701 taaaataaat agaattgtta aattttttag tttcattgtt atctcctgta ttgtaattgg
     5761 aataacggag tgacccaaaa aaggttacag tgaatttgaa tttttttttg agttggaaag
     5821 atgcacacac acgcagatca cttaacaaat gaattattaa aatacagaaa agaactgatt
     5881 gggtttatta tcaaaagaac acaagatcca gatttagcag aagatatatt ccaagactct
     5941 cttttgaaat tagttcgaaa gtctcctgat atagaagaca cacaaagtct acttgcttgg
     6001 ttttataccc tacttagaaa tgcgatcatt gacacctatc gcaaaaaaaa tacagacgct
     6061 aaaaaattag aaagtataaa aaaatctttc caaacagaaa tcaattcaac agacaaaaat
     6121 gatttatgtg aatgctttaa agaaattctt ccaattctaa atccagatta taaagagatt
     6181 attgaagcag acttaagaga agaagataca gatacaacag ccaagcgatt aggaatcgaa
     6241 aaaaataatc taaaagtaaa aaagtttaga gcaagaaaac aattaaagga acgattagaa
     6301 caaacctgtc gaatgtgtgc aaaacatgga tgtctagatt gcacctgtaa aaaagactcc
     6361 agtcactcaa attaaaaaaa acttttctct aatcattaaa acttatatcc aagtgtgaac
     6421 caatagtttc taccttcgag tggcatgagt gttggagttc cgagtccctc tgcagttcta
     6481 gctcctggat caaaatattg tttatttagt agattgttaa tctgtaattg aataaacaaa
     6541 ccttcccaaa aagcatcttc ccatctgata tttgttttta caaaaaatat ttggtgagtt
     6601 gttttgattg gattcgtcgc aacgtttctc ctaacgtcaa gccaattagt tcccatataa
     6661 aaagaaagat tttgtattat attccaagta attccagaat ttaaaatatt ttctggatat
     6721 aaaggaattc ttccggattc aggaacaaaa ctacgattgg tttttgcatt tactaaatcc
     6781 aaggcataat catctcctgg tctaccttcc actgctggag aggatgtaat aattctagat
     6841 agatttacaa aatcaccttt cgtatgcgcg tagttgaaat gaattctaag attttttagg
     6901 atagcagttt cattgcttaa ctcaaaaccg tagattttac tatctcccgc attttggttc
     6961 tgactccaag ttccgtttgc aggtctcagt ttagttactg gatcaactga cgcatcatct
     7021 gtctttactt ctaagataat gtcatgaact cgattgtagt atccattgag agtaaaaaag
     7081 tatttattta aaactctata ctcaaatcct aactcatagg atttcattct ttcaggagaa
     7141 agcctagtat tttcctttct agcagatgta gcactaaaaa gttcccaccc actaggtggt
     7201 ctaaatccag tcccataaag aatcttaatc gtgaaattct caaagggttt aataattgtt
     7261 ccaagtcttg gattataggt ctttccgtat atcgtatttt catcaaatct atatccaagt
     7321 gtgaaagaaa caaatgatac aggagaataa atcgcctgtc catacaaacc gtaattagaa
     7381 tatttaaaac tttgatatga attatagcct cttggcacta ctgtataact catttcagtt
     7441 ccaatcgaaa gagagagtcg agaggaatgt tcataggtaa atttttctct cagttcattt
     7501 ccataatcgg gtcttgttct aggattgaga gtcacatcat ccggtttata gaaagcatca
     7561 ggacctggat ttcgatagat tccctctaca ctcgaattta ataaaacagt cgatcttgaa
     7621 attacttcag aatctaaact cattttttta ttaatagtcc ataaatatgc catatagact
     7681 gaattacttc taaaattcca atatgcgcca ggctgaccgt tagccgtaat ctgaccattt
     7741 aaaattccaa gtcttctaaa gttgtttcta gagtcaaaat tatcagaatc caaacccttg
     7801 tcatgataat ctaatcgtct agttccattt tgaaaatttc ccgttccagc aatatattga
     7861 tggtttacag attcaaatct aaaccctttg taagccagct ttcctgtaat attgtaagta
     7921 tcgacatccg caatattata tcggtttgac caataatatc cgactgattt tgaatcaggc
     7981 tgacattgac cgccacaagc ttttgattca tagtaataag ccgcatcatt tggatttaca
     8041 tttggtttat ctaatctttt ttgatctcca aagtatggtc cttctgtttt ataaagatat
     8101 gcagaggcgc tatagtgcat tgcttgttca cctttaccaa tgctatttct acttgttata
     8161 ctcgttgaat aaccaggatt acgaccgtta gactcccaag ctccatacat agcagtaact
     8221 tcatttccag gtcgatcttt cccagtttta gttataatat tgatcactcc attaaatgca
     8281 tttgctccat agagagagga agcgggacca ttgataatct caattcgctc tacattctgt
     8341 aaagggaatc gtagagttcc ggcaaggatt gaattttcag aaagtatatt ttcgggaatt
     8401 ccatctacat aaacaatcga tctctgattg ctattattaa tcattcccct ttgaaacatc
     8461 aaatcagacc atacaccata agctcgaata aaattaaacc ctggttggtc tattagagca
     8521 tctgttaaaa ctcgataccc tctttctctg atttgctttt cggtaattac ataaactgca
     8581 gcgggagcat ccgatacttt ctgttccttt ttggcggcag tcacaacaat attttcttcg
     8641 agtctaaaga agtcctcgtc agtcaatttt ttagattcat tcgattcttc cgattttgca
     8701 ctttcttgtg attcttctaa aaatttcttt ttttctgctt caaattcgga tttttctttt
     8761 ttaaaatttt ctttttcttt ttctaactcc tctctttcct ttttgatctt ttctagattg
     8821 gtattatcaa tctctttttt ttgggcgaaa agatttgaag aatagatcag tattaaaaat
     8881 ggaaatagtg ctagtttttt catgacgagt ataagctgtt aagtgaacca agtattgaaa
     8941 attagaaaga aagcgtcaat tttttttcaa aatctagctc aagaggtagt atctaatttt
     9001 acctagactt agtctcggtt tagcgcttat acgggataat gcctattacc catgctatct
     9061 tatgcttgta aaatatttaa gtaaatcagt tggccaatct ggagttccgg atttcaatat
     9121 ggtaattttt ccttctttct gaccaatttt atctaagagt aaattggtta cggtcttagc
     9181 cataacgttt gttttctcca taaagacaga atgctgatct acaaaaaccc aatcgcctat
     9241 aaaaagtaaa ttctctatag gagactgtat ctcaggtcga tttgcttcat ccccaggacg
     9301 aaatcccgta taagtatccc aatgattgat ataaaactca gaaaactctt ttaaatcagg
     9361 taaaatttgt ttgagttctg tatgaacaat atttgcaatc tctctgtggg aatatttcaa
     9421 aagttcttgc catttcgata cttgcacttc gattaaatcg atatttttct ctctcaattc
     9481 aggaatttgc tttgaagacc aattcgtggt aaatcctaaa ttttcgtaac cagtgggcat
     9541 aaaatcataa gcactgtatt tgccttcagg aaatcttttc ttccaagcat cactattttc
     9601 ataaaataaa tttacacaaa gaatatgagc attcgtgagt aaagttgctt ttttgaaata
     9661 gtctccttct ttatcaagcc ctgtgccaag tataaatttc ttggctcctg gtatatccat
     9721 tgccgcaata aaataatctg ctttgacttc taaaggtgtt gtatctttta aaatttctaa
     9781 ttcagaatga tgcgctccac aaaatggaca atgctcatag tctccatcgc ccattatatt
     9841 tccacaaact ctacatctct ttgttctaga tttaggaaaa ttttcatttg ttttaatcga
     9901 gacaattttt ccattctcgc ggataatact tgttacctca tgattaaaat aaatttcccc
     9961 tcctttggag agaatatgat ttatcatagg ctgtataaaa gtttcatcgg gcggccattt
    10021 atataaatct actttggaat ctttgggtag atttccaagg cgcataaaat ttacaatcgc
    10081 aagagcggaa cattcttttg tggtcataaa gaatcccatt tctgctaacg catcaaagaa
    10141 atgctttata tattctacac tcattccatt ttttactgcc cagtcataaa aagtaatact
    10201 atccaaataa agtcgatctt ctttattgga aaaatcaaaa ccccacatct tcatagaagt
    10261 tctgagtgtt tcaaatagat tagaagcagg aactctaata tcctctctgg atggaacata
    10321 gattccattg tcatacattt caattcgatc aaaaggaatc ggccaggtag tgttttgaat
    10381 tttgctttga actccacgct ttgaaataaa ataataaaag gaatcatctt ttcccagctt
    10441 attaatttca attttatgac gtcctagaaa ttctcttagg tttttataga atccccaaat
    10501 cgcatgaaga ccatgttctc tagaataacc cttctctcca aaatgctttt tcgcaaaatg
    10561 cttatccttc caagttttga gttttccacc cgcataggaa gttttttcta caaggactac
    10621 tttaaatcct ctatcagaga gttcgcaggc tgcttgtaat ccggcaagac ctgcacctaa
    10681 aatacataca gtcttaccgt taagagaaag acttacatga ttttcgggca gatgaggaaa
    10741 gtctttttta tattcagagt ttgcaaaaaa agaatatcca attccaccgc ttaaaccaag
    10801 ccctactccc gttgctccta cttgtaagga tttttttaga aattcttttc tgttcattga
    10861 gctattgctc ttgcacgata tgtattgatt gcatataaaa catagaagat aacagtaaaa
    10921 aataaagctg tatctatcgt ttgtttataa atggtaaatc ggaatacagt tgcaagtaaa
    10981 atcatactaa gtctgagaag gataattccc atgtgaatct taaaagaagg tttctttttt
    11041 gctaacaaaa agaaatcaat cggactaaac acaaaaatca taatggtctc atccataaat
    11101 ggaaaagagg taaagagtcg aacgacaagc gcaaagagac caccaaaacc tgagataatc
    11161 ccaaaaagaa tttctccctt tcgatcaatc attgatttga agtaaacagg gatttgcccc
    11221 acaatgataa aaactagaat gataaacatt aagttaccta agaaatcaaa agagtctgga
    11281 ggcatttgcc aaaaatttct atgtaaaagt tctttgtctt ttacaagctt tgcatcttcc
    11341 actgcaaaaa gtaaatcata aggaaggtaa attaattcgg ttggatttct ttctttatct
    11401 gtatcatgat cgagtaatag tttttcttcc attctaagcc aaaaaatttt attagaataa
    11461 ggaataactc tatttctcca agttctttta ttactttctg tttctagttt gagaggtgtt
    11521 ccatatgttt ctccaattaa gtctcggata taggtaacac aatttcctgt gaagttgtta
    11581 tattcatacc catctttgta tttctcaata aaggaaagta tgttttttaa aaataatttc
    11641 ttttgatttg catctaactt aaattcagaa gcatacatcc cacgacccgt tgtatcccac
    11701 tgcatataag cgagacccat cgtttttaca gtgatgtaga atttcgcttc cccttttaag
    11761 aatctccaga taaatcctgc tgactcatcg tattcaccaa aatcgagata gaaatcctta
    11821 tcctcaaact gttttccgag aaaaactcgc aaagcagaat gtccaaaagc agttgtgact
    11881 gtaggagaag tatctactgt aatcagttga atcttcaaat cctctaaatc ctttggggtt
    11941 aaagaattga taaaagagac aaatttgtcc ttattttcaa tgttaatttt ataatcacct
    12001 tgtgagaaaa tggaattagt aactaaaaaa agaaaaatta aaaaatatcg aaacatttac
    12061 ttaccctgtt tttcaacgat atacgctatg aatgctttga tcctttcttc cggcattgga
    12121 actctgaaat agtgatcgtt tctccaagag ggaaagagag gaactccctc aatatggctg
    12181 tcagaaagta aattcaaacc ttcattcggt ccaaattgag ctaaataatt ataaaaaggt
    12241 ctacctcttt ctgttacaag tcttgctgtc ggaactccta caatgtttac aacagtcatg
    12301 tttttaggga tttccaattc tttactgaga ggtgcatttt ctcctcctcg catagaagta
    12361 agaccttccc aattataacc cataacccaa aaatagaatt taccttccca tcggtaaagc
    12421 caatgatcca tcattccatt tactacaagg cttcctttgt tgattccacc gaagttaaac
    12481 caaccgacaa ccttagaaaa ataaggctct tttccacaaa gttccaatgc ttttttaaaa
    12541 tcaccgcttc ctttgctggc tgaaccaatg ataattcctt tgtattccgt ctgacttttt
    12601 ataaaatcac aaataatctt accgttaccg tctaccgttc cagtttggtc tgtcggaatt
    12661 acagcttctc caagtcccaa ttgttttact attgcgcgga tcaattttcc atctgcatcc
    12721 acctctttat tatctcggta aaacatccct ggagcgattg caaagagcat tttcttggag
    12781 gaatagtcgg gaactttcct cataaattca ttctgttttg tataaatata ttctataaac
    12841 ttacgatttg ttggttctgt gatagctcta tgatagaaaa cagcagccgc tttgtcaata
    12901 tcgttggtct ctttcatgat attgaataga acttctcttg taagatcaga taattttctg
    12961 tatttaggat aagaggcagc ccaatctaag tattcttttt cgtcttcgca gatataatcc
    13021 acatagtaat catttccttc acatctaaaa ccttttagtt gctctgttgg atcgttaagt
    13081 tttctgtctg cagtacatga gagtattgta ataagtataa gtattgtata aagtctgttc
    13141 attttgtaag tcttgaaaat gtaaaataag atgaaaatcg agaaggaggg aaaatttgaa
    13201 atgctacgaa agatcgtagc attccaaact caaaatatta ggtaccgtta acgaggatgc
    13261 agctctcaac taatacagaa ccgagtaagc tattgatgct tgcgtctaca gattgaactt
    13321 tagtaattcc accttgttta gcagctgcag caatactcat atctccactt ctgattaatc
    13381 cgaggatagc gttagtgcaa gcttttccag tttttgttcc aactggctta ccaccatctt
    13441 ttccccatac gcaaccagct tcaccagcgt ttgtgatatc tccaccccaa gtacagtttt
    13501 ggttagattt aacgttgtcc atgatagaac cagaagaagc tggttgaata gatggacctg
    13561 cgccagatcc gcttccaccg ctacaagcga tagcagctgc aattgcaagt gctgttaaaa
    13621 cacctaatga atttttgaaa tttttattca tgtttgtctc cataaaaatt atatgaaaag
    13681 gaaatatatt tcccttccaa tgttttttac ttcacttttc aacgataatt ttgtcaatga
    13741 aaatcatgca aaaagtaata aaaacttcaa ttttttgctc cagctttttt aagttgattc
    13801 acaatatcgc cttgactatt ctcaattgct aaggctaacg cggtttgtcc gcttttatct
    13861 tttagagtta aatcatgttt cttacttaaa agtagagcag tcatctctaa attcccttgt
    13921 aaaactgatt tcataagagc tgtttggcct ctttcatcct gcatatttgg gttagctcca
    13981 gcggatagaa gttgacgaat gatctccatt tctcccatat ttaccgccca aattaaaggt
    14041 gtatagccag aagaatcagc caaattgaga tctgcttttg ccttgataag agcagaaacc
    14101 atatcttttt gtcctttttt tgccgcaatg atgagcgctg tctttccatc atttgctttg
    14161 ctatttacat ccgctctgtt ttgaattaag aacttaatcg aatcaatatc atttttgtag
    14221 accgccttaa tcagtgcggt ttctccgaat aggtctgtag catttatatc cgctttttct
    14281 tttaatagaa tgggaagtgc ttctgtgaat tgaaagttaa ctgctaacat aagaggtgta
    14341 actccctctt tatttcttac attcacattt gccttagact ttaatagctc ttttgtgatt
    14401 tctaatcggt agcgtgctat cgcaaacata agtgctgttc gctgtaaagt gtctaccgta
    14461 tttacatccg ctcctttttc gagtaaaagt tttacaatct catcatatcc ataattacat
    14521 gcattcatga gagcagttcc atttaggttg tttgttgcat ttacatctgc tccagcatca
    14581 agcaacattt tcacaatatt tgtataacca aatccagcgg aatacataag aagggttcca
    14641 ccgaagtctt tgtcttttac gtttacatcc tttttttcat ttatatactg ggagattttt
    14701 ttttcatctc cattgcgtgc agcttcaaat agttttttat tccccgcaca attcaaaatt
    14761 gagacaagaa gcccaataaa gagtaaactt tttccaagat tttgcatata acgtcctttt
    14821 ttaattatga ttccatttgt ttatggttcg ttgaaaattg aataataagt gcgataatag
    14881 aacttactat gatcaaaagg gtaatcacct gagcataggt aagactacca aagattaaat
    14941 tcctatctcc tctaaaaaat tctacgaaga aacgattggt tccgtagagc gcccaaaaaa
    15001 taaaaaataa ttttcgcttt gcccattctt ttttatcaat tcgaaccatg ataaataaga
    15061 ctataaacat cagagccatt tcatagagct gtgttggatg cactttagga tagacaacag
    15121 ggaaaggaat taggcctagt tttaaaagtt cttgtttata atcgcctgta gcagatggag
    15181 gataatagcg agttaactct gccagatttt taaatcctaa atcttttaga tgagacattg
    15241 agtcgggaat cggcatatcc gcaagattta catatttgct aaacatttgt gagatgatag
    15301 caagagggtc ttcactattt gcatagaatt gatgtaaatg tctccactct gccatccaag
    15361 catcactgtt ttcagaaaat gtaactccta aaatggaagc agttggtaga ccataggcat
    15421 ctccatttag aaaacaagac atacgactta gagcaagacc aaccgttagt ggaagcatta
    15481 aactatcgca ccaacttaaa aaatcttttt tagtatactt acagtatgcc caacctccga
    15541 atgcagttcc aatggtagca cccaagatgg caaatccacc tgtggaagta aaaataatca
    15601 ttggattatt tagaaaatta gttgtctccc aaaatgcaaa atggaagagt ctagcaccaa
    15661 taattccaga accgacagcc gccgcccaag cagaattaaa ccattcaata tcatatcctg
    15721 ctttcagaga tctatatctt tgatagaaat aaccaaagat taggatcccg atagcccaat
    15781 gaccttcata gcctctaaaa tgaataaaat accaatcgat atttactgga aacaaaatga
    15841 ctcctagtta ttagattgtc taaatttctt tgtgcactct aaaaagaatt ctacttcttt
    15901 tttactacct gtgacaagag tagttctttg atgaagactt gtaggctcca tgtctaaaat
    15961 tctttcacca gaaacggtaa ccgccatacc tcccgcttgt tctgctagaa atgccatagg
    16021 agatgcttca tagagaagac gtagtttgcc gagtggatag ctttttgatt ttgtatcttc
    16081 tgggtagaga aatattccac cttttagtag atttcgatgg aagtcggcaa cgagagatcc
    16141 aatgtatcga agagtctttg gtttattatt gttctcgata tttttaatgg ttctaatata
    16201 agattttacg gcatcagacc agaaatcata gtttccttca ttgatggaat agatagaacc
    16261 tgtttctggg attttaatat tttcatggga taataggaat tcaccgcaag aaggatctaa
    16321 agtaaaaccg actaccccat ttcccataga taaaaccatc atagtggaag atccatagag
    16381 aatgtatccg gcacatcttt gaagtgcacc tctttggagt aaatccgatt cttccccggg
    16441 tcctccatgt ttactctttc gaagatgaat cgaaaagatg gttccgatcg atacattgga
    16501 atcaatattg gaagatccat ctaatgggtc aaaggcgaaa gtatactttc ccattttata
    16561 acccgacgga atttcaatga tggattcatt ttcttcgcta gcaagaacac agaggtgacc
    16621 gcaaatactc aaagcttttg taaatagaac atctgcataa cgatctaatt tcatctgtgt
    16681 ttcaccttgg acattagttt ccgaggttgc cccaagaact atatcagtga gtcctgcttt
    16741 tcgaacttca cgagagacaa ttttggctgc atacacaaga tgttgcataa gagctgtaaa
    16801 atccccagtt gcatacggga gttttaattg ctcttctatt aaatattgag aaagacttat
    16861 catttcagtg gctgatttca tacttatttc aggttttaaa tcattttcaa atgagcaagg
    16921 aaattatttt aaatcagatc atatcttgac tcacaaatga gaagattcga ttcaaatgaa
    16981 tccctcgtgt gaggtgatgc aatggataga aaagctccaa gaattcttgc ttattctgcc
    17041 ttttttcctt ttttattcag tcccaatctc attcaaaatc agaatcaatc actatgaact
    17101 acttgaaaat aagaaagaat agctcctctt ttgattcact ctctccttgg tatttacacg
    17161 aaatttatgc agatttatcc caattcatct gaaaattctt tcacttttct tttccttttg
    17221 tagagattgc actctgatcc tttgaaaatc aatttagata taaaaaattt ttctttcact
    17281 taggaatact cgaaagaaac tatctgcatg actgatttaa ggaaattact tcttatgact
    17341 cttacaacaa ttggaattgc aacccagctt tctagtgatg aaggcatgtg gactttagac
    17401 aatctcccca caaaacaact agaagaaaaa tacaatttta aattaacaga caaatggtta
    17461 aaaaatgctc aactcgcttc cgtgcgattt aatgacggtg gttctggggc ttttatttct
    17521 gcaaagggtt tagtgataac aaatcaccat gttgcaatgg gtcaattgca aaagatgtca
    17581 tctaagaaaa cagattatgt aaaagatgga ttttttgcac aaaaggaaag tgaagaaatc
    17641 aaatgcccag atttagaatt aaatgttctt atttctatgg aagatgtcac aaaacaagtc
    17701 gtaaccgcat ctgaaaaagg cgataccgat aaagcaaaaa atgaaaaaag aaaagaagaa
    17761 atcgcaaaga tagaaaaaga atcaaacgag aaaacaaaac tcagatccga tgttgtagaa
    17821 ctctatgatg gaggagaata ttggctttac aggtataaaa aatacacaga cgtaagactt
    17881 gtaatggctc ctgaattgca agctgcttcc tttgggggag atccggataa cttcaattat
    17941 ccgcgctttg cactcgatat ggcttttttt agagtgtatg aaaacaacaa accaatagag
    18001 tccaaaaatt atttcaaatg gagtaaagaa ggtgcgaaag aagacgaact tgttttcgta
    18061 tttggacatc cgggctctac agaaagacaa aagacaatga gccaaatcca ttaccaaaaa
    18121 gagtatgcat ttcctgaata tgtaaatctt ctaaaaagaa aattaaaact atatagagac
    18181 tattccgaaa aaggaaaaga agaaaagcgt cgtgcgaaag actatatctt aggaatggaa
    18241 aattcactca aagcaatcga aggagaatta agcggtttaa aagataaaaa aatactagat
    18301 tcaatggaag aaaaagaaaa taaactaaaa agtctagttt cacaagataa agaattacaa
    18361 aaagaatacg gagagatttg gaaccgaatt gattctgtgc aaaagaaatt aatcgcaaaa
    18421 cacaaagagt tctattatag aagtgtaagc aatacaagac ttgtgaatat tgccttgaaa
    18481 ttagtccgat atgcgacaga gatagaaaaa ccaaacgaaa aaagatatga ggaatataga
    18541 gactctaatc tagaatcact caaatttaga cttctctccc ctgccccaat ttatacagat
    18601 atggaagaga tgtcacttgc ccatacttta caattggcag tcgagaagtt aggcaaagac
    18661 gatgagtttg taaaaattgc tatcacatca gatccaaaag aattcgcaaa gaatttaatt
    18721 acaaaaacaa agttaggaga tgtagcctac cgcaaagaac ttttaaagga aggaaaagca
    18781 ggaatagaga aatcaaaaga tcctctgata gaatggattc gtaaagtaga tccaatcctt
    18841 cgaaaagata gagattggtt tgatgatgaa atagaaagcg tgcaagcaat tgaaggtcat
    18901 agactctccg ctttaaaatt taaagtattt ggaaagtcca tgtatccaga tgcaaccttt
    18961 actcttagaa tgagttatgg ccaggtaaaa ggttttacag atgatacaac gattgaagtt
    19021 ccttttaaaa caactttcta tggtctattt gatagagctg agagttttaa tcacagagaa
    19081 ccttttaaac tttctacaaa gattttagaa aataaatcca gtatacaaat gaataaaccg
    19141 ctcaatttta tcacaaccaa tgacatcgta ggtggaaact ctggaagtcc agtcgttaac
    19201 cgaaaagggg aatatgtagg tcttatcttt gatggaaata gctatagcca tattctctct
    19261 tatgcctata cagacaacaa agcacgtgct gtctctgtcc acagcgaagg aatcctagaa
    19321 gcactccgat cgatttataa aatggagaat ttggtgaaag agataacggc tgccccgtaa
    19381 atatttgttt tgaatttggc attttcatgt aggcgagaca acgctgtctc gactacacaa
    19441 ggtttcactt tataccgatt gccctaaaaa aaaatcccat gtttttcgaa aagagcctca
    19501 tttttggcaa tcgggaatac ccaaatttta aaaaatggga aaaaactttt ggcgttgggt
    19561 attataaaaa attctctaca aacatttttt gaattttaac tatatcaaca agatgatttt
    19621 gtaatacgtt atacacttct tttttgtcta ttttagcata ttcatgaaca agtagatttc
    19681 gaaatttaat cattcgaatc catagt
//
LOCUS       DAOQOS010000104        19468 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_1401_length_19468_cov_6.259362, whole genome shotgun sequence.
ACCESSION   DAOQOS010000104 DAOQOS010000000
VERSION     DAOQOS010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 19468)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19468)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19468
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1401_length_19468_cov_6.259362"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..708
                     /locus_tag="PLF66_19085"
     CDS             <1..708
                     /locus_tag="PLF66_19085"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HMZ66203.1"
                     /translation="AFYKYTVVQGDGFFRIMARTGMNIDTISSVNALSSPQDIRVGMT
                     LLIPNMRGVYDTDNLPETEISRSKLAQKYDMPENLILFDKRIKEWFIPGRKLGRIEKS
                     FFYGLAFHPPLNEGYLTSGFGTRLDPFTKKKTFHGGIDIAAAKGTDVIASADGEVIFM
                     GRKGGYGKLIVVKHIMGYETRYGHLDKMEVSLGDHVKKGQKIGTVGSTGRATGPHLHY
                     EVRRFQKNEKPVFYQHM"
     gene            918..1121
                     /locus_tag="PLF66_19090"
     CDS             918..1121
                     /locus_tag="PLF66_19090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66204.1"
                     /translation="MLKKKLTDIVFDWCVEFLIYWAKILGISYNEINVYLFCIIWPIF
                     TILLIAIILYQWRIISKLKSKKN"
     gene            1365..1757
                     /locus_tag="PLF66_19095"
     CDS             1365..1757
                     /locus_tag="PLF66_19095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66205.1"
                     /translation="MKEPVCTALLFADRVIVENNGKKGIIGTFNKIYAPMFPVQTAPW
                     GIYAAITNVVGKHNFQVALNHMETNQNILLIPGEMQASNPDEVIELTFNTAGVGFPSE
                     GKYSLSLTVGSELLSSIVLSVEVMKAIG"
     gene            1771..2295
                     /locus_tag="PLF66_19100"
     CDS             1771..2295
                     /locus_tag="PLF66_19100"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HMZ66206.1"
                     /translation="MVRKIFKVSLFLFGFGILAETGLDKSQQDEMVAAHNKYRKAVNI
                     SNIKWSDSLAETAQAYAEKQKGNGCKMKHSGAKGLGENIYWASAISYSNGTTKPQNIT
                     SSKVTDAWGSEKANYNYKKNACASGKVCGHYTQIVWKNTTEVGCGMVVCKDNSQFWVC
                     NYTPPGNYVGEKPY"
     gene            2351..3289
                     /locus_tag="PLF66_19105"
     CDS             2351..3289
                     /locus_tag="PLF66_19105"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004420752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HMZ66207.1"
                     /translation="MSYNSVKHLISWSDWSDEEIQSILDFAVYVKNNRVYFSGHMHGR
                     TMAMLFQKTSTRTRVSFEAGMTELGGHAIYLDWRASNFQLSDIDFEAEYLSRNVSIIM
                     ARMKRHEDLLTLKSGSLVPVINGCCNLYHPCQALADMLTIRLDKGSIKGASLCYVGVH
                     NNVVNSLIGITAALGVHLTLVTPIAPKEAVQDHIVQRGINHKTLSWETDLKHAVNHCE
                     YVYTDTWVDMEFFNEPSYAKEKEERIAMMMPYQLNSDLLKNSKAKIMHDMPIHAGYEI
                     TREIVKSERSIIFQQAENRLDAQKAVILRLLEANAN"
     gene            complement(3298..3864)
                     /locus_tag="PLF66_19110"
     CDS             complement(3298..3864)
                     /locus_tag="PLF66_19110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66208.1"
                     /translation="MKQLILCLLLLTTSTVFADDKKNYEKLKKAPDAATCKQDTALCL
                     AYKYIQHYHSNAKEAEWFQNTAVSHYFDYKITYQNEREKGEIFLSFESYSDGLEWKYW
                     EDSTLIRIKDPLKLVDSKSAQKKIQEKLESEFDKDIKIDRIQKIITRSLMQNQQTPRE
                     YYSIEEKIQLKVKANGKTFVFKSNGDSP"
     gene            4126..4347
                     /locus_tag="PLF66_19115"
     CDS             4126..4347
                     /locus_tag="PLF66_19115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66209.1"
                     /translation="MSNHPKPTKDEFTTAMHETMSIDEESDTKHKVCSFLSSLSWYKS
                     VSLAEQMKSLGITEADVEKYRPEWEALQK"
     gene            complement(4390..4710)
                     /locus_tag="PLF66_19120"
     CDS             complement(4390..4710)
                     /locus_tag="PLF66_19120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66210.1"
                     /translation="MKMFIVLFLFFVLTGLFAETKVAIDCTFKGKRLAGKVRIVTANE
                     DFKVRVVNANEHLKVLLQTLTWNDRCGEWMYVEANENFKIRFVEANEDFKIRFVNAIP
                     GVPN"
     gene            complement(5147..5347)
                     /locus_tag="PLF66_19125"
     CDS             complement(5147..5347)
                     /locus_tag="PLF66_19125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66211.1"
                     /translation="MKILFPFLSNTIAISTFEPIINASCCSIVFKEVLEVILSIIFFA
                     SAISVAVPSIRFPDSDLILDFP"
     gene            5379..5720
                     /locus_tag="PLF66_19130"
     CDS             5379..5720
                     /locus_tag="PLF66_19130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66212.1"
                     /translation="MNGFDIENKIVTHNHPSNTPISLKDFKIFLEGKALEMRATTFQY
                     TYSISNPNKVKLNYIDVENFWKSMYQKAKIQAQIKIDKGLILAKDLLIEIQDIQWELT
                     SKEFKFVYDRK"
     gene            5707..5919
                     /locus_tag="PLF66_19135"
     CDS             5707..5919
                     /locus_tag="PLF66_19135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66213.1"
                     /translation="MTENKSFVLDDSEQRENIGEGNECYSCKHSTDHGVCSAFTYIPD
                     DIWSGKVSHRQPYPGDNGIQFEKIVY"
     gene            5931..6206
                     /locus_tag="PLF66_19140"
     CDS             5931..6206
                     /locus_tag="PLF66_19140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66214.1"
                     /translation="MNFALFTIYWYKDISFTSFRAGLSLLRISKCNSENALLSLTYEE
                     WLDMPRKFLTLSFLWFKFDFYFYEGHWHTISIYDYHKLSLTPCKPMR"
     gene            6419..6604
                     /locus_tag="PLF66_19145"
     CDS             6419..6604
                     /locus_tag="PLF66_19145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66215.1"
                     /translation="MDYYSGMSNREIERKYDLSPRQIQRQCRRIMIRYIKEGGRFEKD
                     SMSVAFLKCLDYDVNRF"
     gene            complement(6921..7415)
                     /locus_tag="PLF66_19150"
     CDS             complement(6921..7415)
                     /locus_tag="PLF66_19150"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HMZ66216.1"
                     /translation="MAIKDLLPARWRKNNDLEARNSNLVDSFTSLQREMNRVFDSFFD
                     NWDFPKVSSSFSPTLDISENEKEYKISVELPGMEEKDIEVHSTEDSLTISGTKKNEME
                     EKKDHYHYFERSYGSFKRTIPLGENVDSSKISASFKKGVLHITVPKNKEYISKQKKIP
                     ILSE"
     gene            complement(7473..15500)
                     /locus_tag="PLF66_19155"
     CDS             complement(7473..15500)
                     /locus_tag="PLF66_19155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66217.1"
                     /translation="MVRRWILFLLIFFFSTQCFVFQKSVKITPANFEYDSIAENYFSR
                     GNEKPFPLTVQRGNNLYNSTTQDGRYLFYTTDHSGNYDIWFRDLRSAIIVPVTTHPAA
                     EYKPAISPNGKKLVFVSEENDSAGDIVLINLSPTDLLERHLAGEKNLAEEKIFLTNPN
                     YADSKKKDNFVDTDPAWSPNGKHIVFATDRFTPGLQNLALLDTSNPNQIIQLTMKGAA
                     SPQFSFDGKSIVYLSFQDSKEGEIYRLNLESKTEERLTNDSYMNFSPSLSPDGNFLYF
                     TSIRKDTNKNQVLDTEDNSLIIKLDLTTKEEKILTTDNFSVFDTKFSFFNKGSILFSA
                     ALYNTINIYFIPESGPIPKKANIDEQFNFALRIKKTQDNYFLALDSLKLFFEKDPLFS
                     IYEAKAKDTKIEFKQDRGQQKEAKEILDEMLTLENDPKNALSYAMAIRRIARNSNKSA
                     EKSLFEYYERMKGLNADKQVLASILKLVGGEKERSKNISEAKEHYQTILKNYPDYFEK
                     REVKRKLARLEFNEGSITIPELYFDLLNDSSTTLEDLRLILNDLETKISANKNYIQKI
                     EYTDKLLANNKLKERSKPLYELVTYIKSKALSEGKKFQESNTILDSFLEPVKKNPDCD
                     INPFCQKLLVCVKNPVCLKSHLLKSKNFEGLGSVNGSFDELRVFLENYDPDLGVELNK
                     TEIEKTFRYYENKARDHEARGNSSPENRIHLRDAAFHYFFNVENMYLLKEKNLFVEDL
                     YKDYAVYYQRKMVDSIFNYAEKQAQDNREDLLAKLNVLGDKKLNVFGRVTLLLSNLLD
                     NRVTNKFKFLGDFRDLKQETILGKPGEPDDALKIIDAHFVLSRPRARPVLYLASLYGY
                     AYYLINKSLIYETHYRSTNTMTPARKETILKDLRNAENELKWIIFADPQYADAHQLLG
                     WLYQYIDIFKSTKPSPNEPSDGEVYADIYAKFFSEKHFEENINLYKQVLDMLGNIQNK
                     KILSDLNLNLANNYLLLQNYPKAKEHYTKVETYGQNILDQIQFENYKQAALFNYHYAR
                     SLISMGEYQKASQYLKKTAEIYYDNEYYEEVGKNPAGLGLPKKKKEKVVYDVHNKLVL
                     IYSLLGLSERESGNYINAINAYKKAIALNYNSGYVENINLYSSLALCYQKVEKYHLSN
                     YYISKVETEYKKYKAKQRFKIPSLRDWFWNLILPDRVRVIGDGRFPGEFAPEEHYLLA
                     QGIKIQNLSEEGDLREAEKAITERDKFLKDTGLNKLIIGEQISLNAKYKIGYDNFIRG
                     RYEKALNHYDEFLEQVKSKKIPVGNQTKEKRKILKRKSFTLFRLIESAEANQEYLLPS
                     LSDNINDLNSEKNTSVEACLKTTKQTKQENDLRKFCEDAFHKEWYVFDPLLGLSYYYL
                     GEVYNQQGNYSSAFHYYGLAIPLLKDPASIPVELVGLPGDLFSKTERLRLKINLANVY
                     YRIGDEKQFKAIIGETEELSSEFQAMEDLLKVKLLKAKFLFQKAKTKNDFLKVLEEID
                     AIEKGLTKSYATLIDLEDGFFYELFSLSQETHFKLGNWKEILSKEEKLYSHLLFNHFQ
                     NTRIVFDEEKLEGLNQYLRDTIQKDLENNNKYKVLVNTRKPLSTHLQSRVEITKEVER
                     VIGLILKFFPEKKRFFEMEEATKPETINPNESIIRFKKIQNSLVVSELIQSKESFSSL
                     PIQSSLEETLSSFFARHLEKAQTNEITIIPDKSFANLDFKSIPVKNSTLGNLVNLNYA
                     LTMRQVNPITHKSIQFETIVDTVEPKEGTEPKPSKRLGPILSDSDVLNTKVHYTTGNV
                     FTSNRSGFINLKEFAEKDSTLSLVILNQTKLDDKSFFKTVAAIDSVQSTKVPLLILGG
                     DKPIKINKVIKEELLKENPTSIGWKNTLFENNSSKKYLSVKEEAFKWERQKKYREAIE
                     LFQEANTLVNPADREEKLQSEINLARIKSKGIPKTKRFVFFERLIKKYQDSNPEKITI
                     YEALLHQCYVHFSNTYCRDHFKNYYRTLKSSELDSESKERSKLLVNFYIQVNNGSIQN
                     LEKNYQNFLKNVNFEDLFLFHEDMSDLFIKNFILDKAEFHTKQLIRQANTKEEKQIAL
                     QLENEIELLSYFSQKSNNINPIEVTESIYKDGILKNWNQVEEKIKSLNRRSYDDVLIK
                     YRQRLFSIWKDIHSGREINPLQLISETTSTGKSVYSILSQVDRALLFHLLLNSIPYQT
                     NTEVNTVFDALLENQKEESKKIQNLYLQMSWSEELLNRGDFTSAEKYLSKLETDLNQF
                     PYDHSLETRFHLAKFKLLELDENARKNLKPANPIVLESEVDWYPLYKKAKNKDINSFI
                     PMLNEILAAKSDKPYDSFNKREFLDFITYLQLKAFQSNSSEVFLDLAFYKDKIEDLNR
                     LKKESLNFSSLPQAEVINDLLLKKIPKGQEFIAVVNFGLQTFSIKIENGKSNGELSFA
                     DYRPIKNEIYSYYKQVQKKGSGLLLKDSLEPKFRNKILLKKNKLTYIYLSSFFIKAPL
                     ELKEEDNFYIVQNPALLSRREIRKSSDFFQDKFPIKKFKDLLPEEKILSKLENLEITE
                     RTGSTLPVILSGERLFLASSSELQFGKNTLSNLQTDRRKGVWFLSNSDLYSTSFFKDD
                     LNIALNYLDQIHHGPGIFSLTEQSSPANVLFIKTLLQDTQLQTNLKPRFLEAIKTVKA
                     RYPQEAYWHGYRLYTNTLLEE"
     gene            16001..17275
                     /locus_tag="PLF66_19160"
     CDS             16001..17275
                     /locus_tag="PLF66_19160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020768488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HMZ66218.1"
                     /translation="MRDFFIDRFRYIRRDAIAGIITGLMAIPLSVGICLMSKFPILIG
                     LYTVIFACCIGFIFYLIRPGNYVGTPGIAAGLAPALALGVDSFGMANMPFLVFATACL
                     QAVVWKFNLQRYILKFVPHFLVEGLLAGVGLKIALNFLPYVYTTSNPSHGIFHLDRII
                     VVILSILSLSAFLYLYNKYGKTLPGLSYSSILLFGTISMFFISLPTIEVEQIDFKFEF
                     PMPHGEYFKDKSLAYEVVLMIGYAVVLGSIDVIEQVMSNAAIEKLDPLNRRCDTNNSL
                     LAIWVANLGSSFFGGMTNLDGLAKSSTNAFAGAYTKLSNLFTAAVISVMVIFPHLLSY
                     LPEYTLGVIMIFTGWKMISGLGNVARAYGKYELSLAIVCGLLVFRLGIFEGLLIALAV
                     NILIGFVYHKSRNREISEIWSHIIFHTDEEIG"
     gene            complement(17346..17888)
                     /locus_tag="PLF66_19165"
     CDS             complement(17346..17888)
                     /locus_tag="PLF66_19165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66219.1"
                     /translation="MKRSLPEKLVQMKKKNFHLSHLDGFEFQEADQDTFDSWFNTEKG
                     DHSKFLYSFAQDGTGGLFCLWNESRSNDFQNANVVYLSSEGDFGVLAANFDDFLSVLA
                     GGLNLYDLIFYGNVTPYQDLINDEDELLEAKTNHQKLVKWIQTEFQIRPEMDGPKILQ
                     RGKHLLAPFKKWVVSRMGNY"
     gene            complement(17976..18359)
                     /locus_tag="PLF66_19170"
     CDS             complement(17976..18359)
                     /locus_tag="PLF66_19170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002977079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66220.1"
                     /translation="MNFFMKTILPIFLATFWISISEFVRNEFILKNFWVKHYQSLGLV
                     FSTEPLNGAIWGIWSLCFAITIFILTRKFTFWETILFSWLIGFVLMWLVIGNLGVLPF
                     GILPYAIPLSLLEVFLATLILKKLA"
     gene            complement(18647..>19468)
                     /locus_tag="PLF66_19175"
     CDS             complement(18647..>19468)
                     /locus_tag="PLF66_19175"
                     /inference="COORDINATES: protein motif:HMM:NF019224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1554 domain-containing protein"
                     /protein_id="HMZ66221.1"
                     /translation="TITITRSSTLSSISSISSLSVSLGTLSPTFSSGTTAYTMTVANG
                     VSSITVTPTLTDSLSTMTVNGTSVNSGSASGSITINVGNNTITIVVTAENGNTTSYTI
                     TVTRLNATTKRMFVTANSYNGNLGGVTGADSLCNADTNKPNDGSTYKAVITDATNRVA
                     CSNANCTLATENVNWVIGANLNYIRASDAATLFTSNSAGIFSFGSITNSFDTSSNKYW
                     TGLNANWTSNGSHCSLWANTGGSGMVGNGNQTNNNAISQNTQGCSGTQNLLCAEQ"
BASE COUNT         5967 a         2988 c         3574 g         6939 t
ORIGIN      
        1 gctttttata aatatacagt tgtgcaagga gatggctttt ttagaatcat ggctcggact
       61 gggatgaata tagatactat ttcttctgtc aatgctctta gttctccgca agatattcgt
      121 gtgggtatga cgcttttaat tcctaatatg cgtggagttt acgatacaga caatcttccc
      181 gagacagaga taagtcgttc taaattagca caaaaatatg acatgcctga aaatctaatt
      241 ctatttgata aaagaatcaa agaatggttt attccaggta gaaaattggg aagaatcgaa
      301 aaatcttttt tctatggtct agcctttcat cctcctctta atgaaggata cctaacgtct
      361 ggttttggaa ctagactcga tccttttact aaaaagaaaa catttcacgg tggaattgat
      421 attgccgcag cgaaaggaac agatgtgatt gcttctgcgg atggagaagt gatttttatg
      481 ggacgcaaag gcggttacgg gaagttaatc gttgtaaagc atattatggg atatgaaacc
      541 agatacggtc atttggataa gatggaagta agccttggag atcatgtaaa aaaaggacaa
      601 aagattggaa cagtcgggag cacaggacgc gcaaccggac cacatcttca ttatgaagtg
      661 aggcgttttc aaaaaaatga gaaaccagtt ttctatcagc atatgtaagg tgaatggatg
      721 ctatcatttt agagatttga taatttagga accaaagatg aatttcggaa tattagaaaa
      781 tgctaatgaa ttaataaaat taatagcctg ctgcatagtt tggcttttgg agagcgaaaa
      841 agcgctggga gaaaagccct ccgctaggag agtatgtcta atttcaaaac ttggttcaac
      901 ttatgtaagg tgaatggatg ctaaaaaaga agttaactga cattgttttt gattggtgtg
      961 tggagttttt aatctactgg gctaaaattt taggaattag ttacaacgag atcaatgtat
     1021 atctcttttg tatcatttgg ccaattttta caattcttct cattgcaatc attctctatc
     1081 aatggagaat catttcgaaa ttgaaatcga aaaagaatta aataaagtga gatcgatgta
     1141 acaatttttt caccaccgat gaactgcgat aactgaacga tgaagaaacg atggataatt
     1201 ttgaattgtc gtatctttca tcggtgataa tcgtctttcc tcccaaaatc cattgccgct
     1261 tgttcgcggc aataggtggt aatcttttga tttttctgtt tcaccgaact ggcgttaaat
     1321 attaaacgat tgactcttgt ttgtttgatt cttagattag atgaatgaaa gaaccggtat
     1381 gcactgcttt actttttgct gatagagtga ttgtagagaa caatggaaag aagggaatca
     1441 taggcacctt taataaaatt tatgctccta tgtttcctgt tcagactgct ccttggggaa
     1501 tctatgctgc tataacaaat gttgtcggaa aacataattt tcaggtagct ctaaatcata
     1561 tggaaacaaa tcaaaacatt ttattaattc ctggagagat gcaagcgtca aatccagatg
     1621 aagtaataga actaacgttt aatacagcag gagttggatt tccttctgaa ggtaagtatt
     1681 ctctttcttt aactgttggt tctgagttat taagttcgat tgtattatct gtggaagtaa
     1741 tgaaggcaat cggttgaatc ggaggataga atggttcgaa aaatttttaa agtaagttta
     1801 tttctttttg gatttggaat tttagcagag actggattag ataaatcgca acaagacgaa
     1861 atggttgctg ctcataataa gtatcgcaaa gcggtaaata tttcaaatat caaatggtct
     1921 gatagtttag cagagacagc acaagcctat gcagaaaaac agaaaggcaa tggttgtaaa
     1981 atgaaacaca gtggagccaa aggcttggga gaaaatattt actgggcgag tgccatttcc
     2041 tattcaaatg ggactactaa accgcagaat attacttctt ctaaagtaac agatgcttgg
     2101 ggaagtgaaa aggcaaatta taactacaag aaaaacgcat gtgcttcagg aaaagtatgt
     2161 ggtcattata ctcaaatcgt ttggaaaaat acaacggaag tagggtgtgg gatggtggta
     2221 tgcaaagaca attctcagtt ctgggtttgt aattatacac ctcctggaaa ttatgtaggc
     2281 gagaagccat actaaaaaca atccttccta tttcataaat ctgtagcata gaaaagccga
     2341 tattatatct atgtcataca attcagtaaa acatctgatc agttggagtg attggtcaga
     2401 tgaggaaatt caatctatac tcgattttgc agtctatgta aaaaacaatc gagtttattt
     2461 ttcaggtcat atgcatggtc gcacaatggc tatgctcttt caaaaaactt caactagaac
     2521 tcgtgtttca tttgaggcag gaatgactga actcggtgga catgctatct atctagactg
     2581 gagagcatct aattttcaac tttctgacat tgactttgaa gcagaatatc tttctcgtaa
     2641 cgtttccatt attatggcta gaatgaagcg tcatgaagat ttactcactt taaaatcagg
     2701 ctctctcgtt ccagtgatca acggatgttg caatctatat catccttgtc aggcattggc
     2761 tgatatgctt acaattcgct tggacaaagg aagcattaaa ggggcaagtc tttgttatgt
     2821 aggtgttcac aataatgttg tgaattcttt gattggaatt acagcagcac tcggagttca
     2881 tttaacatta gttacaccga ttgctcccaa agaagcagtt caagatcata ttgttcaaag
     2941 aggaatcaat cataaaactt tatcttggga aacagattta aaacatgcag tcaaccattg
     3001 tgagtatgta tatacagata cttgggtgga tatggaattt tttaacgaac cttcctatgc
     3061 aaaagagaaa gaagaaagaa ttgcaatgat gatgccctat caattgaatt cagatcttct
     3121 gaaaaactcg aaagcaaaga ttatgcacga tatgccgatt catgctggct atgaaatcac
     3181 aagggagatt gtaaaatcag agcgatctat tatattccaa caagcagaga atagactaga
     3241 tgcacaaaaa gcagtaatcc ttagactgtt agaggctaat gcaaattagc ctcataatta
     3301 aggtgaatcg ccattggatt taaaaacaaa tgtttttcca ttggctttta ctttaagttg
     3361 aatcttttct tcaatggaat aatattctct tggagtttgt tgattttgca tgagagagcg
     3421 agtgatgatt ttttgaattc gatctatctt tatatcttta tcaaattcag attctagctt
     3481 ttcttgaatc tttttctgag cagattttga atctaccagt tttaacggat ctttgattcg
     3541 aattagagtt gagtcttccc aatacttcca ttccaaacca tctgaatagg attcaaaaga
     3601 aagaaagatc tctcctttct ctctttcatt ttggtaggta attttataat caaagtagtg
     3661 gctaacagct gtattttgaa accattctgc ctctttcgca ttcgaatggt agtgctgaat
     3721 gtatttatac gcaagacaaa gtgcagtgtc ttgtttgcaa gtagctgcat cgggtgcttt
     3781 ttttagcttt tcgtaattct ttttgtcatc cgcaaagaca gtcgaagttg tgagtagaag
     3841 taaacataaa atcaattgtt tcattctttg attctaaagt agaatagaaa aaagaaaagt
     3901 tttttttatc ttactttgaa aagttgttcc atccatgttt tctaaacttt aaacttaatg
     3961 attccttttt ctggttcgtt ttcttgtgat tcagcaatat gcggagaaac caaacccaca
     4021 acgaaaaaaa aattgaattc gaaacgatgg taataaaaga attcatagaa gaaatcaatt
     4081 cagaatagaa aaggaatttt tagttgaagc tatcaagagg gaattatgag taatcatcca
     4141 aaaccaacga aagacgaatt tacaactgca atgcacgaaa caatgagtat tgatgaggag
     4201 tctgatacga aacataaagt atgctctttt ttaagtagtt tatcttggta taaatccgta
     4261 agccttgctg aacaaatgaa aagtttagga atcaccgaag ctgatgttga aaaatataga
     4321 ccagaatggg aagctttgca aaaataacga ttctaataga aagaagttta tatttcaaaa
     4381 cccttttgtt taattgggaa ctccgggaat tgcatttaca aaacgaatct tgaagtcttc
     4441 attcgcttca acaaaacgaa ttttaaaatt ttcatttgct tccacataca tccattctcc
     4501 gcatctatca ttccatgtaa gagtttgcaa tagaactttc aaatgttcat ttgcattcac
     4561 aactctaact ttaaaatctt cattggcagt aacaatacga accttaccgg ctaaacgttt
     4621 ccctttaaaa gtgcaatcaa ttgcaacttt cgtttcagcg aataatccag ttaaaacgaa
     4681 aaataaaaac aaaacaataa acattttcat acgaacctct ttgatcttag accattgtca
     4741 aaggagccaa agaaggaatt tcatcaaagc ttatcgctaa agagatttct gatttaacgg
     4801 gcgtatctca atctaaggct gaattctggg cacaagatca aagtgcaaag ttctttgggg
     4861 aagtaactcg attcaatcaa acctctgctg gatttgatgg tttcatttgg aggacagtta
     4921 gagattctcg cgttcgtcct gttcaccaaa ctctagaagg aaaattcttt acatggaaag
     4981 atggttctac tgaacctaac gcacaattcc ccggtcgtga ttatagatgt cgttgttttg
     5041 ctgagcctgc gtttaaggat gagtataatg taaatatcga agcacagtat aaggcacagg
     5101 cggatgctca gagggcgatt attactgaga ctactggatg gagaggttac gggaaatcta
     5161 gtattaaatc tgaatcagga aaacgaatac tgggaactgc aaccgaaata gcagaggcaa
     5221 aaaatataat agataatatt acttctaata cttctttaaa aacaattgaa cagcaagagg
     5281 catttataat tggttcaaaa gttgaaatag caattgtatt tgataaaaat ggaaatagaa
     5341 ttttcataac ccaaacagga gggaaaaact ttgtagattt gaatggattt gatattgaaa
     5401 ataaaatcgt aactcataat cacccaagca acacaccaat aagcctaaag gattttaaaa
     5461 tatttctaga aggtaaagct ttagagatga gagctacaac ttttcaatat acttattcaa
     5521 tcagtaatcc taataaagtg aaattgaatt acattgatgt tgaaaatttt tggaaatcta
     5581 tgtatcaaaa agcaaagata caagcgcaaa ttaagattga caagggttta attttagcaa
     5641 aagatttact aatagaaata caagatattc aatgggaatt aacatctaag gaatttaagt
     5701 ttgtatatga cagaaaataa atcttttgta ttagatgact cagaacaaag ggaaaatatt
     5761 ggtgaaggaa atgagtgtta tagttgtaaa catagcacag atcatggagt ttgctcagcc
     5821 tttacttata ttccggatga tatttggtct ggtaaggttt cacatcgtca accttacccc
     5881 ggtgacaatg gtatccagtt tgaaaaaata gtttactgat ggctacatca atgaattttg
     5941 cattatttac aatctattgg tataaggaca tttcttttac tagttttaga gctggacttt
     6001 ctttacttag aatatcaaaa tgtaattctg agaatgcttt gctttcttta acttatgaag
     6061 aatggctaga tatgccaaga aaatttttaa ccttatcttt tttatggttt aaatttgatt
     6121 tttattttta tgaagggcat tggcatacaa tatctatata tgattatcat aagcttagtt
     6181 taacaccatg caaacctatg cggtaaaact tatattaaaa atagacgctc agccaagcgt
     6241 ctctactact cataatttaa cttgacataa tcctagtata gaatctaatt ttattcgact
     6301 aacaagcgac tactgatggt catcgatgta agatgaccga ttttttacat tagaaactat
     6361 gcgacatacg ggaaatctga accttaaaaa taaatcgatt caatgggatt ctcttttcat
     6421 ggattattat tctggaatgt ccaatcgaga gatcgagcgt aaatatgatt tatccccccg
     6481 tcaaattcaa aggcaatgta gaagaatcat gatccgttat attaaagaag gtggtagatt
     6541 cgagaaagat tctatgagtg ttgccttttt gaaatgtttg gattacgatg tgaatagatt
     6601 ctaatttatc catgtaatca ctaaaataga ttataaaatt gagttaaggg tatgaatctg
     6661 gatatttatt catttaaatt tcctctaact tacttttaaa ataatttttt cttggaagaa
     6721 tgtcaattct ataaaaaggg atgcccgaaa gcatccctta aaaaaattac ggtagtagta
     6781 agatgggatt aatacgatcg atttgattcc taacgctttt tttttcgctt tgtaactatg
     6841 ttcttttaga tagaaatttt ggattcaatt atcagagatg aaaagacctc cacagagatg
     6901 tgaaggtctt tttatttata ttattcagaa agaattggaa tttttttctg ttttgatatg
     6961 tattctttgt tttttggaac tgtaatatgc agaactccct ttttgaagct tgcgcttatc
     7021 ttcgaggaat ctacattctc tccgagtgga attgttcttt taaaagagcc gtaggatctt
     7081 tcaaagtaat gataatgatc ctttttttct tccatttcat ttttcttggt tccagagatt
     7141 gttagagaat cctctgtaga gtgaacttcg atatcctttt cttccatacc cggaagttct
     7201 acactgattt tgtattcttt ttcattttca gaaatatcta aggtgggaga aaaagaacta
     7261 gaaacttttg ggaaatccca attatcaaag aaagaatcaa acactcgatt catttctctc
     7321 tgtaagctcg taaacgaatc cactagattg ctatttctag cttctagatc gttgtttttt
     7381 ctccatctag ctggtagtaa atccttgatt gccattgtat tttcctcctt tttagcactc
     7441 tattggttag agtgctaaaa ggtgaaaaaa gttcactcct cgagcaaagt attcgtataa
     7501 agccggtagc catgccaata agcttcttgc gggtagcggg ctttaacggt tttgatggct
     7561 tctagaaagc gaggttttag gttggtttgc agctgggtat cttggagtag ggttttgata
     7621 aacaatacat tggcaggact tgattgttcg gtgagagaga agataccagg accatggtgg
     7681 atttggtcta ggtaattgag tgcgatattt aaatcatctt taaagaaaga agtgctgtag
     7741 agatcagagt tggagagaaa ccatacacct tttcgtctat cggtttgtaa attggaaaga
     7801 gtattttttc caaattggag ttcggagcta gaggctaaaa agagtctttc cccggagagg
     7861 ataacaggaa gtgtagaacc agttctttct gtgatttcta aattttctaa tttagataga
     7921 atcttttctt cgggaagtag gtctttaaat ttcttgatag gaaatttatc ttggaagaaa
     7981 tcagaggact ttcgaatttc tcttctagag agaagagcag gattttggac tatatagaaa
     8041 ttatcttctt cttttaattc gagaggagct ttgataaaaa aggagcttag ataaatatag
     8101 gtgagtttat ttttctttaa gagaatttta tttctaaact tgggttcaag agaatctttt
     8161 aaaagaagac cgcttccttt tttctgaact tgtttgtaat agctatagat ttcattttta
     8221 ataggacgat agtcggcaaa ggataactca ccgtttgatt taccattttc gattttaatg
     8281 gaaaaagtct gtagtccaaa gtttacgact gcgataaatt cttgtccctt ggggattttt
     8341 ttaagaagta ggtcgtttat tacttctgct tgtggaagag aggagaagtt taaactttct
     8401 tttttgagtc gattcaagtc ttctatcttg tctttataaa aagcaagatc taaaaaaact
     8461 tctgagctat tggactgaaa ggctttgagt tgtagatagg tgataaaatc taaaaactct
     8521 cttttgttga atgaatcata gggcttatca ctcttagccg ctaggatttc atttagcatt
     8581 ggaataaagc tatttatatc tttgtttttt gcttttttat agaggggata ccaatcaact
     8641 tccgattcta agacaattgg atttgcaggt tttaaatttt tacgagcatt ttcatctagt
     8701 tctagcaatt tgaattttgc taaatgaaat ctagtttcga gagaatgatc atagggaaat
     8761 tggtttagat ctgtttctaa tttagaaaga tatttctctg cactcgtaaa atctcctcga
     8821 tttaaaagct cttccgacca agacatttgc aagtatagat tttgaatttt cttagattct
     8881 tctttttgat tttctagtaa agcatcaaag actgtattta cttctgtatt ggtttgataa
     8941 ggaatggagt tgagtaataa atgaaataat aaagctctgt ctacttggct tagaattgaa
     9001 taaacagatt taccagttga ggtagtttcc gaaatcaatt gcaatggatt gatttctctt
     9061 ccggaatgaa tgtctttcca gatagaaaat aatctttgtc tatatttgat taatacatca
     9121 tcataggatc ttctatttaa acttttaatt ttctcttcta cttgattcca gttttttaga
     9181 attccatctt tataaataga ttctgttact tcgattggat tgatattatt tgatttttga
     9241 gaaaaataag ataaaagttc aatttcattt tctaattgca atgcaatttg tttttcttct
     9301 tttgtgtttg cttgtctgat taattgtttt gtatgaaatt cagctttgtc taaaatgaaa
     9361 tttttaataa acagatcgct catatcttcg tgaaataaaa ataaatcttc gaaatttaca
     9421 ttctttagaa agttttgata gttcttctct aggttttgaa tggaaccgtt gtttacttga
     9481 atgtagaaat taaccagcaa tttactacgt tcttttgact ctgaatctag ctcggaagat
     9541 tttagagttc tgtaataatt tttaaaatga tctctacaat aagtgttaga aaaatgaaca
     9601 tagcattgat gtaagagtgc ttcatagatt gtgatctttt ctggattcga atcttggtat
     9661 ttcttgatga gtctttcaaa gaaaacaaaa cgttttgttt tgggaatccc tttgcttttg
     9721 atacgagcta ggtttatttc tgattgtaat ttttcttctc gatcagctgg atttacgaga
     9781 gtgtttgctt cttgaaataa ttcgattgct tctctgtatt ttttttgtct ttcccatttg
     9841 aatgcctctt ctttcacaga gagatatttt ttcgaggaat tgttttcaaa taacgtattt
     9901 ttccacccta tcgaagttgg attttctttt agtaattctt ctttgattac cttgtttatt
     9961 ttgattggtt tgtctcctcc taaaattaga aggggaactt ttgtggattg aacagagtca
    10021 attgccgcaa cagttttaaa aaaggattta tcgtctagtt tggtttggtt taagattacg
    10081 agtgatagag tagaatcttt ctctgcaaat tcttttagat tgataaagcc gcttcgattg
    10141 gaagtaaata catttccagt tgtgtaatga acctttgtat ttaaaacatc tgaatcagaa
    10201 agaatcggtc cgagtctctt ggaaggcttt ggttctgtgc cttcctttgg ttctactgta
    10261 tctacaatcg tttcaaattg tatactttta tgagtgatcg gatttacctg acgcattgta
    10321 agtgcgtagt ttaaatttac taaatttccc aaagtggaat tctttacagg aatggattta
    10381 aagtctaggt ttgcgaagga tttgtctgga atgattgtaa tctcattcgt ctgtgctttt
    10441 tctaaatgtc ttgcgaaaaa ggaagagagt gtttcttcca gagaagattg aatcggaaga
    10501 ctagagaagc tttcttttga ttgaattaat tctgatacca caagagagtt ttgtattttc
    10561 ttaaagcgta ttattgattc atttggattt atagtttctg gttttgttgc ctcttccatt
    10621 tcgaagaaac gttttttctc aggaaagaat tttaaaatta aaccgatgac tctttctact
    10681 tcttttgtaa tttctactct agattgtagg tgagtagaga gcggcttacg ggtatttacc
    10741 aatactttgt atttattatt attttctaag tctttttgaa ttgtatctct tagatattga
    10801 tttagacctt ctaacttttc ttcatcaaat acaatccgag tattttgaaa atggttaaag
    10861 agtagatgac tatataattt ttcttctttg gaaaggattt ctttccaatt gcctaatttg
    10921 aaatgagttt cttgagatag agaaaaaagt tcatagaaaa atccatcttc aagatcaatt
    10981 aaagttgcgt agcttttggt tagtcctttt tcgattgcat ctatctcttc taaaaccttg
    11041 agaaaatcgt ttttggtttt tgctttttga aataaaaact tagcttttag aagtttgact
    11101 tttagtaaat cctccattgc ttgaaattca gaggagagtt cttcggtctc gccgatgatt
    11161 gctttaaact gtttttcatc tccaatgcga tagtatacat tggctaggtt gatttttaaa
    11221 cggagtcttt ctgttttaga gaataaatct cctggaagac ctactaattc aaccggaatg
    11281 gaagcaggat cttttaaaag aggaatggca agcccatagt agtgaaaggc agaactataa
    11341 ttgccctgtt ggttataaac ttctcctaga taataatagc taagacctag aagtggatca
    11401 aatacatacc attccttatg aaatgcatct tcacagaatt ttcttaaatc attttcttgc
    11461 ttggtttgct ttgttgtttt taaacaggct tcaacggatg tatttttttc tgaatttaaa
    11521 tcattgatat tatctgacaa agaaggtagc agatactctt gatttgcctc tgctgattca
    11581 attaagcgga aaagagtaaa actttttctt tttaagattt ttcttttttc ttttgtttgg
    11641 tttccgacag gaattttctt ggatttaact tgttctaaaa attcatcata atgattgaga
    11701 gctttttcat atctacctct tatgaaatta tcataaccga ttttatattt tgcattgagg
    11761 ctgatttgct caccaatgat tagtttattt aaaccagtgt cttttaagaa tttatctctc
    11821 tctgtgattg ccttttctgc ttctcttaaa tctccctctt ctgaaagatt ttgaattttg
    11881 attccttgtg ccaatagata atgttcttcg ggtgcaaact ctcctggaaa tcttccatct
    11941 ccaatgactc tcactcgatc tggtaaaatt aaattccaaa accaatctct tagagaagga
    12001 attttaaatc tttgttttgc tttgtatttt ttatattctg tttctacttt tgaaatataa
    12061 taatttgaaa gatgatattt ttctactttt tgatagcaga gagcaagaga gctataaaga
    12121 tttatgtttt ctacatagcc agaattataa ttgagtgcga ttgctttttt ataagcgtta
    12181 atcgcattga tataatttcc agactctctt tctgaaagac caagtagaga atagattaat
    12241 acaagcttat tgtgaacgtc atacacaacc ttttcttttt tctttttggg aagtccaagt
    12301 cctgctgggt ttttacctac ttcctcatag tattcattgt cataataaat ctctgctgtc
    12361 ttttttagat attggcttgc tttttggtat tcacccatcg agatcaggga tcttgcatag
    12421 tgataattaa aaagagctgc ttgtttgtaa ttttcaaatt gaatttgatc tagaatattt
    12481 tgtccgtatg tttctacttt tgtataatgc tcttttgctt ttggatagtt ttgcagtagc
    12541 aaatagttat ttgctagatt taagtttaaa tcagatagaa tttttttatt ttgaatattt
    12601 ccaagcatgt ctagaacttg cttgtataga tttatatttt cttcgaagtg cttttcggaa
    12661 aagaatttag cataaatatc tgcatatact tctccatcac ttggttcatt gggagaaggt
    12721 tttgtggatt taaaaatatc tatatattga tagagccaac caagtaattg atgcgcatct
    12781 gcatattgtg gatcagcaaa aataatccac tttaattcgt tctctgcatt tcgtaagtct
    12841 ttgagaatcg tttcttttcg tgcgggtgtc atcgtattgg tagatcgata gtgtgtctca
    12901 taaataagtg acttattaat taaataatac gcatatccat agagagaggc aagatacaat
    12961 acaggtcgtg ccctaggtct acttaaaaca aaatgcgcat caattatttt taaggcatca
    13021 tcgggctcgc ctggttttcc taggatggtc tcctgtttta aatctctgaa gtctcctagg
    13081 aatttgaatt tatttgtaac acggttgtct agtagattgg aaagtagaag cgttacgcgc
    13141 ccgaagacat ttagtttttt atctcctaga acatttaatt tagcaagtaa atcttcccta
    13201 ttgtcttggg cttgtttttc tgcataatta aaaatggaat ctaccatctt gcgttgataa
    13261 tacaccgcat agtctttgta taaatcttct acgaaaagat ttttttcttt gagtagatac
    13321 atattttcca cgttaaaaaa atagtgaaag gctgcatctc tcagatgaat tcgattttca
    13381 ggactgctat tgcctcttgc ttcgtggtct cttgctttat tttcgtaata gcgaaatgtt
    13441 ttttcaattt ctgttttatt taattctaca ccgagatcag gatcataatt ttctaaaaat
    13501 actctgagtt catcaaagga gccatttacc gaaccaagtc cttcgaagtt cttcgatttg
    13561 agtagatggc tttttaagca gacaggattt tttacgcaga ctaaaagttt ttggcagaat
    13621 ggattgatat cacaatcggg atttttttta actggctcta aaaaggaatc tagtattgta
    13681 ttgctttctt ggaatttttt cccttcgctg agagcctttg attttatata ggtaaccaat
    13741 tcataaagag gtttggatct ttcttttaat ttattatttg ctaaaagttt atcagtatat
    13801 tcaatttttt gaatgtagtt tttattggct gaaatttttg tttctaagtc atttagtata
    13861 aggcggaggt cttctaaggt tgtagaacta tcgtttaata aatcgaaata gagttctggt
    13921 attgtgatag atccctcatt gaattctaat cttgccagtt ttcgttttac ttctcttttt
    13981 tcgaaataat caggatagtt ttttaaaatc gtttggtagt gttcctttgc ttctgaaata
    14041 ttttttgatc tttctttttc tccacccaca agttttaata tggaggctaa tacttgtttg
    14101 tcggcattta aacctttcat tctttcgtaa tattcgaata gagatttctc tgccgattta
    14161 tttgaatttc tagcgattct gcggattgcc attgcatagg agagagcatt ctttggatcg
    14221 ttttctagtg taagcatttc atctagaatt tcttttgctt ctttttgttg tcctctgtct
    14281 tgtttaaatt ctatttttgt atcttttgct tttgcttcat aaatagagaa aagcgggtct
    14341 ttctcaaaga aaagttttaa agaatcgagt gctaagaaat aattatcctg tgtttttttg
    14401 attcgaagtg caaaattaaa ttgctcatct atatttgctt ttttgggtat aggacctgat
    14461 tcggggataa aataaatatt gattgtattg tagagagctg ctgagaatag aatgcttcct
    14521 ttgttaaaga aagaaaactt agtatcaaag acagagaaat tatctgttgt taagattttt
    14581 tcctcttttg tagttagatc tagtttaatg atgaggctat tatcttccgt atccaaaact
    14641 tgatttttgt ttgtgtcttt tcgtatggat gtgaagtata aaaagttacc gtcaggcgaa
    14701 agagaaggag aaaaattcat ataagaatcg tttgtaagtc tttcttcggt tttgctttct
    14761 agattaagtc tgtaaatttc tccttccttt gaatcctgaa aagatagata aacaattgat
    14821 ttcccatcaa aggagaattg aggagaagcg gcacccttca ttgtgagttg tatgatctga
    14881 tttggattgg atgtgtctaa aagagcaaga ttctgaagac ctggagtaaa acgatcggtt
    14941 gcgaatacta tgtgtttgcc gttaggagac caagctggat ctgtatccac aaaattgtct
    15001 ttctttttgg agtcagcgta gtttggattt gtaagaaata ttttctcttc tgctagattc
    15061 ttttctccgg caagatgtct ttcaagtaaa tcggtagggc ttagattgat taatacaata
    15121 tcacctgcag aatcattttc ttctgataca aaaacaagtt tctttccatt tgggctgatt
    15181 gcaggcttat actcagcggc tggatgggtc gtcacaggaa ctataatggc agatcttagg
    15241 tcgcgaaacc agatgtcgta atttccagag tggtcggtag tgtaaaaaag atatctccca
    15301 tcttgggtgg ttgagttgta taaattgttt cctctttgaa cggtcaatgg gaaaggtttt
    15361 tcatttcccc tagagaaata gttttctgca atggaatcgt attcgaaatt ggctggtgta
    15421 attttaacag atttttgaaa aacaaaacac tgtgttgaaa aaaagaatat taaaagaaat
    15481 agaatccaac gtcttaccac aaacaacatc ctttttgaaa atagtggagt tttcaaccca
    15541 aacttttctg ttttctgaat tttatttagt ggaatactta gtaaaagggg ctcaaatcaa
    15601 atcgaattta ggtttagttc gattaacatc tattatccgt attttcgatt gaggaatgat
    15661 ttctatacaa aaaaggatca aattgagctt attttttgat ctaaatttac ttacagggaa
    15721 tttaacactc aatataacta tagaaaatag aatgaaaaga tttttggtat ttttatggtg
    15781 agcgtttttt aggaatagga atcttgattg ttctcaattt tttcttttcc atattttaaa
    15841 taaaatcttt ctttagcttt atataccaaa tagggttgaa aatggcatat atcgcatcga
    15901 atctctctaa tggtggcttg acagaaattt tagcgcatac taatttattc tatttctaat
    15961 ttataggttt tgtttttagg tttatataga tggggatttt atgagagatt tttttataga
    16021 tagatttaga tacattcggc gagatgctat agccggaatc ataactggac ttatggctat
    16081 ccctttgtca gtgggaatct gcttgatgtc taaatttcca attctaattg gtttgtatac
    16141 agttatcttt gcttgttgca ttggttttat tttttatttg attcgacctg gaaattatgt
    16201 tggaactccg ggaattgctg ctggtttggc acctgccctt gcgttagggg ttgattcctt
    16261 tggtatggca aatatgcctt ttttagtttt tgcaactgct tgtcttcagg ctgttgtctg
    16321 gaaatttaac ttacaaagat acattttaaa atttgttcca cactttctag tggaaggact
    16381 tctagctggt gtcgggttaa aaattgcgct taactttctt ccttatgttt acacaacttc
    16441 aaatcctagc catggaattt ttcatctcga tcgaatcata gtagtgatcc tctcaattct
    16501 ttctctatct gcttttctct atttgtataa taaatatgga aaaaccttac ccggtttatc
    16561 ttattcttct attttactct ttggcacaat tagtatgttt tttatttctc ttccaacgat
    16621 agaggtagaa caaattgatt ttaaatttga gtttccaatg cctcatggag aatacttcaa
    16681 agacaaaagt ctagcctacg aagttgtatt aatgattgga tacgctgtcg ttcttggttc
    16741 aattgatgta attgagcaag tcatgagtaa tgctgcgatt gaaaaactag accccttaaa
    16801 cagaagatgt gatacaaaca atagcttact cgcaatttgg gttgcgaatt tgggatcttc
    16861 tttttttgga ggaatgacaa atctagatgg attagctaag agttctacta acgcatttgc
    16921 aggagcttat acaaagcttt ccaatctttt tactgccgct gttatttctg ttatggttat
    16981 ttttccacat ttactttcct atcttcctga atacacattg ggagtgatta tgatttttac
    17041 tggttggaag atgatctcag gtcttggcaa tgtggcaaga gcttatggta aatatgaatt
    17101 gagtcttgcg attgtttgtg gacttttggt atttcgattg ggtatatttg aaggattact
    17161 cattgcactc gcagtgaata ttctaattgg attcgtctat cataagtcaa ggaatcgaga
    17221 gatttctgaa atttggtctc atattatttt tcatacagat gaagaaattg gctaatgaac
    17281 atccgttcgt tgtaacataa aattttcgaa acaaattctt gtttatcaat tttatctagc
    17341 attagctaat agtttcccat tctagagaca acccattttt taaaaggggc taataagtgt
    17401 ttgcctcttt gcaggatttt gggtccgtcc atttcaggtc gtatttgaaa ttcggtttga
    17461 atccatttta ctaatttttg atgattggtt tttgcttcta gtagctcatc ctcatcgtta
    17521 attaaatctt gatagggtgt aacattccca taaaaaatca aatcatagag attgagacct
    17581 cccgcaagga cagacaaaaa atcatcaaag tttgcagcta acactccgaa atctccttct
    17641 gagcttagat agacaacatt tgcattttga aaatcattgg aacgagactc attccataaa
    17701 caaaacaatc ctcctgttcc atcttgtgca aaggagtata gaaatttact atgatctcct
    17761 ttttctgtgt taaaccaaga atcaaatgtg tcttgatcgg cctcttggaa ttcaaaacca
    17821 tctagatggg aaagatgaaa attttttttc ttcatttgaa caagcttttc gggcagactt
    17881 cttttcatga gtgtcctcgt cggaaaagct atataatacg attttaaaaa ctgagcaagt
    17941 agaaattgat ttttataaaa tgaatccgaa aaaatttatg caagtttttt tagaatgagt
    18001 gttgctagaa atacttctaa tagactaaga ggaattgcat aaggtaagat tccaaagggc
    18061 aatactccta ggtttccaat tacaagccac attaaaacaa atccaatcaa ccaagagaaa
    18121 agaatggttt cccagaaagt aaactttcta gtgagaataa aaattgtaat agcaaagcaa
    18181 agtgaccaaa ttccccagat agcaccattg agcggctctg tagaaaatac aagccccaga
    18241 ctttgatagt gttttaccca aaagtttttt aaaataaatt catttcgcac aaattcggat
    18301 atactaatcc agaaagttgc aagaaaaata ggtagaattg ttttcataaa aaaattcata
    18361 attgctccag tttaaaattt aggtgtgata tttggttctt gcagatctca gtaaaccaat
    18421 acaaagaagg ggcttttttg agtggaatca actagtggtt taaattcacc agccaagtct
    18481 ttagatccgt ttcacactga attcagtttg atatagagat aataggattg agaataaaat
    18541 aacattttgt gacgttaggc tatattcaca ttcgaagagt ctctcaaatt atttctatag
    18601 aatagaatcg atctgcaaca aattttttaa tttacacaat ttaaaattat tgttcagcgc
    18661 ataaaagatt ttgtgtgcca gaacaacctt gtgtattttg actaattgca ttgttattgg
    18721 tttggtttcc atttccaacc attccacttc cacctgtatt tgcccaaaga gaacaatggc
    18781 ttccattgga agtccaattt gcattgagtc ctgtccaata tttgttagat gaagtatcga
    18841 aagaatttgt tatacttcca aatgaaaaaa tacccgcact gtttgaggtg aaaagagtgg
    18901 ctgcatcgga agctctgata taatttaaat ttgctccaat cacccaattt acattttctg
    18961 tagctaaggt gcagttggca ttactacaag cgacacgatt ggttgcatct gtgatcactg
    19021 ctttgtaagt gctaccgtca tttggtttgt ttgtatctgc attgcaaaga gagtcagctc
    19081 cagtaacacc acctaggttt ccattatagc tatttgctgt tacaaacatt cttttggtgg
    19141 ttgcatttag tcttgtaaca gtaattgtat agctagttgt atttccattt tcagccgtta
    19201 cgactatcgt aattgtattg tttccgacat tgattgtaat actaccagaa gcggatccag
    19261 aatttacaga agttccatta acagtcatag ttgagagaga atctgttaga gtcggagtta
    19321 ctgtgatcga agatacacca ttcgctactg tcattgtgta tgcagtagtt ccagaggaaa
    19381 aggtaggact taacgttccg agcgatacac ttagactcga aatagaacta atagagctaa
    19441 gagtagaact ccttgtaatc gtgattgt
//
LOCUS       DAOQOS010000105        19453 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_1402_length_19453_cov_5.946902, whole genome shotgun sequence.
ACCESSION   DAOQOS010000105 DAOQOS010000000
VERSION     DAOQOS010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 19453)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19453)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19453
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1402_length_19453_cov_5.946902"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..698)
                     /locus_tag="PLF66_19180"
     CDS             complement(<1..698)
                     /locus_tag="PLF66_19180"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66222.1"
                     /translation="MRQIDIGSALEKTTSDLLKAEDAAINFIPKNKTLPSIENLKEII
                     ELLRTIIFPGYFGKNNTNSAMVAYSTVGNLEKLYEMLSQQIHNGLWFDFHALDDNIRE
                     KSERLTLKFIESLPRIRRLLFTDAKAMYDADPAAKNIAEIIFCYPTIRAMMNHRIAHE
                     LLKLNIPLLPRIISELAHSETGIDIHPGAVIGENFCIDHGTGVVIGETAIIGNNVRLY
                     QGVTLGAKKFSLDES"
     gene            1085..1690
                     /locus_tag="PLF66_19185"
     CDS             1085..1690
                     /locus_tag="PLF66_19185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66223.1"
                     /translation="MIDFHFKTLDDEILVTLKTDVTEIGTFHRMASVIFFLGWDILSG
                     EILTINENGTDFAIDTLRLKADGNPGSKKAVEIGIMMDAIFSKRKGLDELFQNYEART
                     PNIRNFFRERAELIFQDEIEKQQTCFYIEADSGRGLLYHVTKVLLENKINILSGLIET
                     DEITQRAKDTFYLVDHEGKMFGGTQKAEEIRSQILKPLFNQ"
     gene            complement(1779..2345)
                     /locus_tag="PLF66_19190"
     CDS             complement(1779..2345)
                     /locus_tag="PLF66_19190"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HMZ66224.1"
                     /translation="MNRELLEQKEKVDFESILNVHGKNEDGQTPLHMAASRGHVELVI
                     SLIERGAELNSKSNFGYTPLHYAANQSGVDIAKVLIESGAEVNAQSDFEYTPLHYAAH
                     HGKTEVVKLLVENGATLNKQNTFGFTPLHYAATHGRFDAAKILIEKGAALHLEDEDGN
                     TALDLAKRSGYADIAEIIETALNSDKFI"
     gene            complement(2692..3714)
                     /locus_tag="PLF66_19195"
     CDS             complement(2692..3714)
                     /locus_tag="PLF66_19195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66225.1"
                     /translation="MNKKLLILIITIITNCTAVAYDSAKDSIDAENEKNGSKPFCDYL
                     KLNYFTGRTTSTGFVSSYLQMSASVDTKKYTNQDCLLILTAFPYTPPGIDNEIPTYSF
                     DTGLPSGWSGTWTIDSSSINCYSNSCLKSGSIGNSASTITSLYATTSSGSISFYRKVS
                     SEMSYDYCKFSIDGVSQDGSGDSGSINWTKYRYSISSGTHVFSWTYLKDSGGTSGSDA
                     CWIDNITLPTNSTTVSGGATCSSATALTYNVSRTGSVSTGSYVYYTFTASISSSYTVV
                     VTANTGDPDLYMTKNGSGCPTASTSSYSSTVSGSESYPIVLSSGDVLTIGVYGYSSSS
                     YTVKVQ"
     gene            complement(3825..5111)
                     /locus_tag="PLF66_19200"
     CDS             complement(3825..5111)
                     /locus_tag="PLF66_19200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66226.1"
                     /translation="MKSRNILLIVFCYLLLGWNETGLSIGTTQIDAIEFGAVKTKDCK
                     TKGPATIFSMLKESSCEFYSTVVKPIQKCYDMYGSNSLKGFQCVTGLNEELKYFTKPK
                     ICDTQELEDAEAITCNSWDSLQKVIGCTNDKAVGESRTCLKNTIVNYYKQIQATGNSI
                     FSKVRDNIKGIPDFDALAAAGGRDPIFKVYNAPSPNALAIKDIRSQIDSYTGSGFLAK
                     IEQGVKSDAIEQRKMEEERIERERQERIAREEQQRQQRLADLENQKRQMENEYYQMKS
                     TGLKTPSKCQQAKAEYAQAEANMRQAANFSRGMGSFATGDAANAWNNTANAFDAGANV
                     AETLKNAISIGCVMAGEGDFVDMANAIGNLNNSIQTLKSSSNSGSTYSAPSNPTSSAP
                     DRCHCTLKRGNGISMDLGYINRSACTSSDVVTAICN"
     gene            complement(5113..5550)
                     /locus_tag="PLF66_19205"
     CDS             complement(5113..5550)
                     /locus_tag="PLF66_19205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66227.1"
                     /translation="MKKYYLILFIILIGNCISIQKGEGKEKINTPAEEAYKCENKRGY
                     YAPFLQCQKEYYVKQLTIREEYLKNSSSDSQNQEILQKLDEILERKDYKRALSISASI
                     IKNSTDEDLKSRATDIAVRAYTELQMQDFNKAYPESKFTLKEK"
     gene            complement(5547..6341)
                     /locus_tag="PLF66_19210"
     CDS             complement(5547..6341)
                     /locus_tag="PLF66_19210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66228.1"
                     /translation="MRVNRNKFLNKKETKTIVLICKFSVGFLISIFLLSCGTSDRKDK
                     ALESDAKEFLKSIRKYIIVAGTLNPTSDSKSSPNNEINVKVLQATGKSFAADAYGEVN
                     PNAIFKDGFKEALEKLGNKRAKEVNPLDLGLISPAAGETASDRQRWLKTAGIDAVIEV
                     NYSNLVLDYNTRVPDLGILNIFYTFFLLDLWWIDMAGIWSLEGDVSITVTKVSNGNKY
                     SGNSVYLKTSTGKFFVSRIGNDAFTTSLSEITKSAIVNMIQSGSSL"
     gene            6614..7270
                     /locus_tag="PLF66_19215"
     CDS             6614..7270
                     /locus_tag="PLF66_19215"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HMZ66229.1"
                     /translation="MDFINNKKFVFGIIEDDLNFQSLLTGLLKTRKEVDRIHIFNSAE
                     DALVSSSLPYLDILIVDYRLGGMDGIRFLANPAIRNLEIPKLILTGFNAEEKIFEALK
                     YGATGYMLKEEIYSLDSILEILLTGGAYISPMIALKVANYFKEMEESIDDLNALTSRE
                     KEIIHELANGFSPKEISESLDISVLTVRTHIRNIYKKLEVSNQVQLLKKAKELHFLKE
                     "
     gene            7391..7804
                     /locus_tag="PLF66_19220"
     CDS             7391..7804
                     /locus_tag="PLF66_19220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66230.1"
                     /translation="MSSYNHFIFSNLDANQLLDRIYNSIQNTFSKRNKKPNIHLENSK
                     IEIQYSDYLFTFLLIDEDWVLEESKDIAERFAKTEIQKEVIYHSKKRVEFWGNDDYQM
                     NYFNDYLILLENIQKDSDVIIFDTINGKFFDEIGL"
     gene            complement(7820..9880)
                     /locus_tag="PLF66_19225"
     CDS             complement(7820..9880)
                     /locus_tag="PLF66_19225"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM-DISM domain-containing protein"
                     /protein_id="HMZ66231.1"
                     /translation="MGKFFYLISILILLALGFVRNTNRIFPQKIQSEAVYGSISLKDW
                     NFSEDGMIPLQGEWEFYWERFLYESDKPSSVPKDILELEKRIISIPSEWQNQEFNGKT
                     LPPFGYASYRLKVLLPPHNKPLGLFMTDASSSYQIFINGKLELNMGKVGKIREESLPF
                     LKYDSILIPPQSSDELEIVIQVSNFENLKAGLWNKPFLGTWKDIEQKKNFSLYLALMT
                     VGSLFIMGIYHLFLFKSRPKERSTLYFGLFCLTIMTRIFSMEERVILDVFPFLSFDFV
                     FRLEHITIYFGLPVFLLFVYTLFPKDIFFPFVFLISLLGGSCTILGLVGPMPYYSYYS
                     LIVMQVCLILSVVYSYYVFILAYIRKRPEAFAMLLGTSVFYILLIHDILYGMGIIRTN
                     HLTPYGVVFFTFIQAAMLSTKFSRAYKLSEKLTEQLLEKTNSLSYINSELVDLKSDLE
                     NKVAERTLELEESYKQNYLEMEKVSALEKELVVKKERERIFVDIHDNLGGQLLSLSFL
                     LNSLQADTIVPLESKERLTHMIDEILKSLRGRLLAIEDLSSIEKNFLNGFHLFLLRRY
                     SSANRKISFLADENYSESFIEKERYHGLFSVIQEIINNDLKYGEGQSHWKIQVEEEFI
                     SFQMQALSQYHNRKKGTGYGQDTILKRTQQLGGIFQDSLKDNEYKAELRVPKSNQIES
                     FIMR"
     gene            10734..11834
                     /locus_tag="PLF66_19230"
     CDS             10734..11834
                     /locus_tag="PLF66_19230"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HMZ66232.1"
                     /translation="MFTTIRNDERGLLFKDGNYIKTLKPGKYLLAASLGHTLIRMDVN
                     KAFVTEKNLNLFLKDQSLVEELSIVEVNEFEICLHYEDNLLANVLKPGRYAFWNILKK
                     HEFKLIDTRNPIPNQEFNQSLFTNIKLNGFYYAFQVENHEAGLLYFNGVFQSILKPGK
                     YYFWAGVITANVIKIDLRQQQLDINGQELMTEDKITLRLNFTSQYKITNPLKVMEIKN
                     YQEQVYILLQLSLREYVGALKLDELLQKKQEIADFVLTKLKEKSNDFGIEFVFAGLKD
                     IILPGEIKDILNLVLIAEKKAQANIITRREETASTRSLLNTAKLMEENPTLYRLKELE
                     YIEKISEKVNSISLSSSSGILEQLASIVNIKK"
     gene            11836..13905
                     /locus_tag="PLF66_19235"
     CDS             11836..13905
                     /locus_tag="PLF66_19235"
                     /inference="COORDINATES: protein motif:HMM:NF020084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HMZ66233.1"
                     /translation="MFKVGIGQSDEIDTLCATEKAIAQARNSLGSFIPQACILFAGLD
                     FEHEKILEEINKTFPDINLIGCSSGGEITSEFGFTDDSIVILLLYSDEVSFSIGLGYD
                     SEMNSEIAANNALEMARKNLTNEEKLCLVFTDSFSLSGNKIIQTLNQKVTKGCKIFGG
                     VSARPFVSNLKTKQFFKDKALSQSVVVMLIGGPIEYKYSLSHTWKPIGRKAIATLVEG
                     KKLIKIDDMRALDYYHYYLGDFSFPAPEFPLAVYQNDSEDFYIRVPGGYNKEDGSVLF
                     AGEIPQGSTIQLTEAMRDYIVDDLDQESIRQKKMKINFEPAMAFAFSCIVRKQILGTK
                     VKEEIRVLKEHFPPSFPIFGFYTYGEISPLESEESSNLHNCTMVTLVIGTKSTSNQTE
                     KKEANQDYPEESPNLSDSEIIEEQKNEILKLRKKLEREHSYRVNLENVKDFHGSVLKT
                     INHEIEAAKQVIQQKNVELEKLYKALESEKKKSDDLLLNILPTQVAEELKQNDFTKPV
                     YYESASVLFTDFKGFTKIAGKMSPSELVTELDFYFSEFDRVIEKYGLEKLKTIGDAYM
                     CASGLPKINSNHAIDLVNAALEIQEFMLNLKKEKGESVWELRIGINSGPLMAGVIGKK
                     KFSYDVWGDTVNIASRLESNGEAGKINISQYTYELIRDHFICEYRGKIAAKNKGEIDM
                     YFVLGRK"
     gene            13906..14718
                     /locus_tag="PLF66_19240"
     CDS             13906..14718
                     /locus_tag="PLF66_19240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66234.1"
                     /translation="MRGILLLLFLSYCVTVDESIDGKAKSIQFNVCFWRAEKLNSKIL
                     NDTKNLLVTLGSNCDSISFTEIKADNEGLPNELENIFSKLGYNYTCLDSFHDEKTHLT
                     HTKYITCANNNKVVEIKPFEYADTRRDFSSPPTFFFLELTNGTKANILPFHAHAGNKN
                     ELIDFERVVNFSFQQFSERKTFLGGDFYTDKKYHSESFLKSLLYFQILKNLILEPTTI
                     SGEKNDILMTDKITALNCKGNTLIKYSSSSPVSKELELVSNHFPIIAQCKLK"
     gene            14755..15822
                     /locus_tag="PLF66_19245"
     CDS             14755..15822
                     /locus_tag="PLF66_19245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HMZ66235.1"
                     /translation="MLGIRFIKVRPTDYAILYKKGQIRNEGAGISFFYYAPSSNVVIV
                     PAASCDSPFIFKETTVDYQEINIQGQFTYRVLEPRKLAGLLDFAVDVNGRYTGDGFEK
                     LPVRLMNVIQVTIREKLSGLDLRTALKSGSEFVSHVKSRLKDNESLSALGVEVVDFAI
                     LKISPTPEMSRALEASARETLLKEADEAIYVRRNFAVEQERKIKENELQTQIAIEEKN
                     RKIREEQMNIEIAVQEKKSLLEEARMNSQKALQEKENEIEAQRLKAEIQREEDRKLLV
                     NHQSENIIQTSKAKAEAIRLELNALTSLTPEFLEILASNQMNSQQIVSRAMRELAKNA
                     DKIGNLNITPDLLNNLLENNK"
     gene            15871..17682
                     /locus_tag="PLF66_19250"
     CDS             15871..17682
                     /locus_tag="PLF66_19250"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HMZ66236.1"
                     /translation="MYYVLFLFISISVFGQTTTNLVKKVSISNFSNYKTNPDSKMQEN
                     IYKSLSNRFKENGYTVDQLTESSLEKKLATAKANGSSFLIDGYYKKTSGENLNIFVQI
                     YNTENGKLIDAFNQSPESLPELGQITLDKSEMTITDDKAIEDMSKKVFTRIRANPKRL
                     ENRENINEYVAQAPIGNEMNIPIPQEDVQKASEDVFKLLSEDNSISIVSKRVGQSESV
                     DRTSAIVSVISRSRIVDSGARNLADILKSVPGLEVFYDQFGFYKVALRGIRSKSGMLL
                     MIDGHRMNNFYDGSTFIDIRADAIEKVEIIRGPGSSVHGTNALVGVINVVTRDLGSGK
                     NSQGVFSTRAGSFNTFEPTAFYSRKIGESDWRFTTYAGSYQSDRQQNRIPYDDSCART
                     TPTAGQPFGGLSNACSNPGYTVLPLYASPWVKTNDQKRQNNLFLKLQNGRDGFYITGK
                     VLNETRGPNMGEIATVTPSSELNFNFMNGAMGINKLNITEKLLISGKVYGDSYARRDD
                     IQVEREDYRNHTFMSARKKISYEYSTKGIESVIQYNALSSLVVMLGFQFEKLAVKNFY
                     TQQNYLSTTTAPLPGLLPFFWDYDGTQKNQNREIYTR"
     gene            17636..18613
                     /locus_tag="PLF66_19255"
     CDS             17636..18613
                     /locus_tag="PLF66_19255"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HMZ66237.1"
                     /translation="MMELKKIKTERSIQGEYIQVIWDPLQWLSITMGLRQDRYSDFGR
                     TLNPKGGIVIIPFEKISAGTLILKILGGSAFRAPTFQELYDRTQQYQIGGIFGNDKLK
                     PETIRTIEIGADYNTPYKPLSILLNGYANKIYNNIDGFNQSNVFPGRTDTYVNLRGIS
                     MIGYEVEARLNYSAKNYGYANASWVQVLDSGGIPPNDKVDTVTFRIDVPQARANIGIN
                     HEFTKYFTLNNSVWISSERGSNSRYPSEAQDNRAFRIPQYHIWNVSLATTDVVSKNLN
                     FALNIFNLNNYKLYDDINNVTSAFYPNRVIPSGYLWGRYIEFKLTYFLN"
     gene            18624..>19453
                     /locus_tag="PLF66_19260"
     CDS             18624..>19453
                     /locus_tag="PLF66_19260"
                     /inference="COORDINATES: protein motif:HMM:NF014871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HMZ66238.1"
                     /translation="MNKLIFLLIINLFFCIQKKAPPANALLATSATTNVSVGAPKSIG
                     LDLVTNNRSWNRGCETRVGNLIADSFAWKANADIGTANGGNVRDDQNVPTIKKGTIPD
                     NTLFGKFLIFKNQIYLTQINAYRLKQALENSNRQLTTTRIAASTDDNDVDGPQHGNCY
                     SSGSGSGRLLYVSSNLSVDVTVANTPMTVTGSAASNNLATGVTGARITRMFVKGVMIY
                     NNPTGSFTSGWANGTSSCTVKQTSFTNSAACNQYTLASVDFQVNGGDGNFSFNPDVAP
                     "
BASE COUNT         6680 a         3312 c         3226 g         6235 t
ORIGIN      
        1 gattcatcta atgaaaactt ttttgctccg agcgtaactc cctgataaag ccgaacgtta
       61 tttcctataa tcgcagtttc tccaattaca acgccggttc catgatcaat acaaaaattt
      121 tctcctataa ccgcacctgg atgaatgtca atgccagtct ctgaatgcgc aagttcagaa
      181 ataattctag gcaataaggg gatatttaat tttaaaagtt catgagcaat tcgatgattc
      241 atcattgctc ttatcgtagg gtaacaaaaa attatttcag caatgttctt cgcggccggg
      301 tcagcatcat acattgcttt tgcatctgta aataataacc gacgaattct tggtagtgat
      361 tcaataaatt ttaacgtcag tcgttcgctc ttttcccgta tattatcatc taaagcgtga
      421 aaatcaaacc aaaggccatt gtgtatttgc tgagaaagca tttcatataa tttctctaga
      481 ttaccaactg ttgaatacgc aaccattgca gagttggtat tattcttccc aaagtatccg
      541 ggaaatatta tggttctaag taattcgatg atttccttta agttttctat cgatgggaga
      601 gttttattct ttggtataaa atttatcgca gcatcttcag ccttcaataa atcactcgtc
      661 gtcttttcca aagcagagcc aatatcaatt tgccgcataa attcctccct attttattca
      721 atataatgaa gtatattaaa tatattaaag catttaagat aatataacag atgtataata
      781 taatatacta gatatatatt caatatatta gtcaattaaa aaaataaaaa taaatgagat
      841 cctagagcag caagcaaagc ttcttccatt ttgaaatgga aatgcattat tcggaaatag
      901 ctaagatttt taaaatcata ggaagattag tctgcaattt gtggattaaa atcaaaggaa
      961 tgagattaag aaattatcta aaacactatg aaaaaaatct tatacaaaaa gccctttctt
     1021 ttcgttggat tgttaaacga atttctaatt gataatattt gaatcaaata aatactaact
     1081 agcgatgatt gactttcatt tcaaaacttt agatgatgaa atactagtta ccttaaaaac
     1141 agatgtcact gaaattggaa cctttcatag aatggcttcc gttatttttt ttctaggctg
     1201 ggatatttta tccggagaaa tcttaactat caatgagaac ggaactgatt ttgcaatcga
     1261 caccctaaga ttaaaagcag atggaaatcc aggctccaaa aaagcagtgg agatcgggat
     1321 catgatggat gccatttttt ctaaaagaaa aggcctagat gaactctttc aaaactatga
     1381 agcgagaact cccaatattc gaaatttttt tagagaaaga gcagagctta tttttcaaga
     1441 tgaaatagaa aaacaacaaa cttgttttta tatagaagca gattcaggta gaggtctgct
     1501 ttatcatgtc acaaaggtct tactagaaaa caaaatcaac attctatcag gtctcataga
     1561 aacagacgaa attacgcaaa gagcaaaaga tacattttat ttagttgatc atgaaggaaa
     1621 aatgtttgga ggaactcaga aagcagagga aatacgcagc caaatattaa aacctctctt
     1681 taatcagtaa aaagaaaagg ggttccaaag aatcatcttt aaaacccctt gcaactatcg
     1741 gcaaaacccg atagttctta tgtctcttag tttacctatt aaataaactt atcagagttt
     1801 agtgcagtct caattatttc tgcgatatct gcatagccgg atcttttagc aagatctaag
     1861 gcagtgtttc catcctcatc ttctaggtgc aacgctgctc ctttttcgat taagatttta
     1921 gctgcatcaa atctaccatg tgtagctgca taatgaagag gtgtaaatcc aaaagtattt
     1981 tgtttgttta aagtagctcc attttcaaca agaagcttta ccacttctgt tttaccgtga
     2041 tgggcagcat aatggagcgg agtgtattca aaatcgcttt gagcattcac ttctgcccca
     2101 ctctcaatta agactttcgc aatatccacc ccactttgat tggcagcata gtgaagcggt
     2161 gtatatccga aattgctttt agaatttagt tcagcacccc tttcgataag cgatatgact
     2221 aattcaacat gaccccgact cgctgccata tgaagaggag tttgaccatc ttcattcttt
     2281 ccgtgtacgt ttaatatgct ctcaaaatct actttttctt tttgttctag taactctcta
     2341 ttcatcgtcg ttttccatcc tgtttaatat gttttggggt ccttctggct atcgcgccta
     2401 tctctgcttc taaaaagact ctttttctat tattttagaa ggaataaaaa caaaaaagtc
     2461 aaagcttatt ttatttttta taatcattat agattatgat atttctcata atcttttgca
     2521 acgttagttt tttaaagaac gttagatttt gagagaaatc tgatagaacc caattttttt
     2581 ttgttttgat cacgttaaaa attcttcttt aaatgtaaac tcattaaatt ctttgaggct
     2641 gagtttctga cccattaagc tgccgaaata acctcaaaga tgtctcaaaa ctcattgcac
     2701 tttcacagta taagaagatg agctatatcc atacactcca attgttaaca catctccgga
     2761 tgataagact atcggataac tttcagaccc tgaaacggtc gatgaatagc tacttgttga
     2821 agccgtcgga cagccagagc cgtttttagt catatataaa tcagggtctc ctgtgttcgc
     2881 cgttacaact accgtataag aggagctaat tgaagcagta aatgtataat aaacataact
     2941 tcctgttgaa actgagcctg ttcgggaaac attgtatgta agtgcagtcg cagaactaca
     3001 agttgcgcct cctgaaacag ttgttgaatt tgtcggtaga gtgatgttat caatccaaca
     3061 tgcatctgat ccacttgtcc caccgctatc tttcaaataa gtccaagaga atacatgtgt
     3121 gcctgatgaa atagaatatc tatattttgt ccaattgata gaaccactat cgcctgaccc
     3181 atcctgactc actccatcga ttgaaaattt acaataatca taggacattt cagaagaaac
     3241 ttttctataa aaactaatcg agccagatga agtggttgca tacaaggatg taatagttga
     3301 cgcactattt ccgattgatc cggattttaa acaactattg ctataacagt ttatagaact
     3361 tgaatcgatc gtccaagttc cagaccaccc cgatggtaga cctgtatcaa acgaataggt
     3421 tggaatttca ttgtctatac caggtggagt ataaggaaaa gcggttaaaa taagtaagca
     3481 atcttggttt gtatattttt tcgtatctac cgatgcactc atttgtaaat aggaactcac
     3541 aaagcctgta gatgttgttc ttccagtaaa ataattcaac ttcagataat cacagaatgg
     3601 ttttgatcca tttttctcat tctctgcatc aatggaatcc ttagcagaat catatgcaac
     3661 tgctgtgcag tttgttatta ttgtaatgat aagtattaat aattttttat tcattgtatt
     3721 tgcccttgca aagatatgtc ttcgcagctt tttatatttt ctgttttcat actccaatcg
     3781 ttctaatacg aaaggagttg atttgtcaga atatttagtt tggtttaatt acagatagca
     3841 gtaacaacat ccgaggacgt gcatgcactt ctatttatat acccaagatc cattgaaata
     3901 ccatttcctc tttttaaagt acagtgacat ctatcaggag cagaacttgt tggattgctt
     3961 ggagctgaat atgtagatcc tgaattacta gaactcttca atgtttgaat agaattattc
     4021 aaattgccta tcgcatttgc catatctaca aaatctcctt caccagccat tacacaacct
     4081 atgcttatgg cattcttcaa tgtctcagca acgttagccc ctgcgtcaaa agcgtttgca
     4141 gtattgttcc aagcatttgc agcatcaccc gttgcaaaac tacccatacc tcttgaaaaa
     4201 ttagctgctt gtctcatatt tgcctctgct tgtgcgtatt ctgccttagc ttgttggcac
     4261 ttgctaggag ttttcaaccc agtgcttttc atttgataat attcattttc catttgcctt
     4321 ttttggtttt ctagatcagc aagtctttgc tgtctttgct gttcttctct tgcaattctc
     4381 tcttgtcttt ctcgctcgat tcgttcctct tccatttttc tttgctcaat cgcatcactt
     4441 tttacacctt gctctatttt cgcgagaaac ccacttccag tataactatc tatttgcgag
     4501 cgaatatcct tgattgctaa tgcgttaggc gatggtgcat tgtatacttt aaatatagga
     4561 tctcttcctc cagccgcggc aagtgcatca aaatctggta tacctttaat attatctctt
     4621 actttagaaa aaattgaatt tccagttgct tgaatttgtt tataataatt tacaatggta
     4681 tttttaagac aggttcttga ttctccaact gccttatcat ttgtgcatcc aattactttt
     4741 tgaagggagt cccatgagtt acaagtaatg gcttctgcat cctctaactc ctgggtgtca
     4801 caaatttttg gtttagtaaa atattttaat tcttcattta aacctgttac gcactgaaaa
     4861 ccttttagac tatttgaacc atacatatca tagcatttct gaatcggttt tacaactgtg
     4921 ctataaaatt cacaggagga ttcttttaac atagagaata tagtcgcagg accttttgtt
     4981 ttacaatcct ttgttttgac agctccaaac tctatcgcat cgatttgggt tgttcctatc
     5041 gacaatccag tttcattcca tcctaaaagt aagtagcaaa aaactattaa taaaatattt
     5101 ctagatttca ttttatttct cctttaaagt aaattttgat tctggatatg ccttgttaaa
     5161 atcttgcatt tgcagttctg tataagcgcg aaccgcaata tcagtagctc tagattttaa
     5221 atcttcatca gttgagtttt ttataatact cgcagagatt gagagagctc ttttataatc
     5281 ttttctttct aaaatctcat caagtttttg gagtatttct tggttttgcg aatcactaga
     5341 actatttttt aaatattctt ctcgtatcgt tagttgcttt acataatact ctttttggca
     5401 ctgaaggaaa ggggcatagt atcccctttt attttcacat ttataggctt cttcggcggg
     5461 ggtattaatt ttttccttcc cttcaccttt ttgaatggaa atacaatttc caattaaaat
     5521 aataaacaaa attaaataat attttttcat agagaactcc ctgattgaat catattcaca
     5581 attgcactct ttgttatctc tgaaagactc gttgtaaaag cgtcatttcc aatccgactg
     5641 acgaaaaatt tccctgttga ggtttttaaa tagaccgagt tacccgaata tttattcccg
     5701 ttagagactt ttgtaaccgt tatactcaca tctccctcta aagaccaaat acctgccata
     5761 tcgatccacc agagatccaa gagaaaaaag gtataaaata tattaagaat tcctaaatct
     5821 gggactcttg tattataatc caaaactaaa ttagaatagt ttacttctat aactgcgtca
     5881 atccctgctg tttttaacca ccgttgtcga tcagaagcag tctctccagc agcaggcgaa
     5941 attagtccta ggtctaaggg atttacttct tttgctcttt tgtttcctaa cttttctaat
     6001 gcctctttaa atccatcttt gaagattgca tttggattca cttcaccata cgcatcagca
     6061 gcaaagcttt tacctgttgc ttgtaatacc ttaacattaa tttcattgtt aggtgatgat
     6121 tttgaatcag atgttggatt taatgtgcct gccacaataa tatattttct aatagatttc
     6181 aaaaattctt ttgcatcgga ttctagagcc ttatcttttc tatcacttgt gccacaagac
     6241 aaaagaaaaa tactgattaa aaatcctact gagaacttac atattagaac tatcgttttt
     6301 gtttcttttt tatttaagaa tttgttccta tttactctca taaaataatc ccgtttaatt
     6361 actttactta aaggtatatg attacgaggt atcagtcaat cgtcagtatt aggtagattt
     6421 ttcatttttt tcatttattg gttttaggaa ataatcaata ttgagtaatt tttattacaa
     6481 agagaaaatt ctgaattgcc agatttggta gaaataatct atcgtgaaat tagctaatta
     6541 ttgcgatagg cgatttacaa caaagaatca ttctcaatat ttagttatac tttgaaatag
     6601 tagagtaaat agaatggatt ttataaataa taaaaaattt gtatttggaa ttattgaaga
     6661 cgatcttaat tttcaatctc tacttacagg tcttctaaaa accagaaaag aagtagatcg
     6721 aatccatatt tttaattccg cagaagatgc gttagtctct tcatctcttc cttacctaga
     6781 tattctaatt gttgattatc gactaggcgg tatggatgga attcgttttt tggcaaaccc
     6841 agcaattcga aatttagaaa ttcctaaatt aattttgacc ggttttaatg cagaagaaaa
     6901 aattttcgaa gcactcaagt atggagcaac tggttatatg ttaaaagaag aaatttattc
     6961 gctagactct atattagaaa tcctattaac aggtggtgct tatatctccc ctatgatagc
     7021 tttaaaagtt gccaattatt ttaaagagat ggaagaatca atcgatgatt taaacgctct
     7081 cacttctagg gaaaaagaaa ttattcatga gcttgccaat ggtttttctc caaaggaaat
     7141 ttctgaatct ttagatataa gtgttttaac agtaagaacc catatcagaa atatctataa
     7201 gaaattagaa gtgagcaatc aggttcagct cttaaaaaaa gccaaagaat tacatttctt
     7261 aaaagaataa attatacatc tccccaagcg agagtctata aaaaatatca aaatccaaat
     7321 ctacaattcc atttagaaaa aatttttgac tttttagcta taaaaataaa acactaaaat
     7381 taggtttaat atgagttctt ataatcactt tattttttcg aatctagatg ctaaccagct
     7441 tttggataga atttacaatt caattcaaaa tacattttca aaaaggaata aaaagcctaa
     7501 tattcattta gaaaactcga aaattgaaat tcaatactct gattatcttt ttaccttttt
     7561 attgatagat gaagattggg tattagagga atctaaggat atagcagaaa gatttgctaa
     7621 aacagagata caaaaggaag tcatttatca ctcaaagaaa cgagttgaat tttggggaaa
     7681 tgatgattat caaatgaatt attttaatga ctatttaatt ctattagaaa atatccaaaa
     7741 agattctgat gttattatct ttgatactat caatggaaaa ttttttgatg aaatagggct
     7801 ttaataatac ctattcctac tatcgcataa taaaactttc tatttgattt gattttggaa
     7861 ccctaagttc tgctttatac tcattgtcct ttaacgaatc ttgaaagatc ccacctaatt
     7921 gttgggttct cttgagaata gtatcttgtc catatccagt tccctttttt ctattgtggt
     7981 attgggatag agcctgcatt tgaaaagaaa taaactcttc ttctacttgg attttccaat
     8041 gcgattgccc ttccccgtat ttaaggtcat tgttgataat ttcttggatc acactaaata
     8101 gcccatgata tctttccttc tctataaaac tttctgaata attttcgtcg gctaggaaag
     8161 aaatttttct attagcagaa ctatatctcc tcaacaaaaa tagatgaaat ccatttaaaa
     8221 agtttttctc tatacttgag agatcttcaa tcgcaagcaa tcttccacgc aagctcttta
     8281 gaatttcatc aatcatatga gtgagtcttt ccttagactc taagggaact atcgtatctg
     8341 cttgtaggga gtttagcaag aaagaaagac tgagtaattg tcctcctaaa ttatcatgaa
     8401 tgtctacaaa gattcgctct ctttcttttt taactactaa ttctttttct aaagcagata
     8461 ccttctccat ctctagataa ttttgcttgt aactttcttc taactctaaa gttctttcag
     8521 ctactttatt ttccaaatca gacttcaaat ctactagttc actattgata taggaaagag
     8581 aattcgtttt ttctaaaagt tgctctgtta atttttctga taatttataa gctctagaaa
     8641 atttggtaga aagcatagct gcttgtataa atgtaaaaaa tactactcca tagggagtga
     8701 ggtgattggt tctaatgatt cccattccat ataagatgtc gtgaatcaag agaatataaa
     8761 acactgaagt tccaagcaac atggcaaatg cttctggtct ttttctgata tatgctaaaa
     8821 taaaaacata gtaagagtaa acaacactca aaatcaaaca aacctgcatc acaatcaatg
     8881 aatagtagga ataataaggc attggtccta ctagtcccaa aattgtgcag gatccaccga
     8941 ggagagaaat aagaaaaaca aatggaaaaa aaatatcttt tgggaaaaga gtataaacaa
     9001 acaaaaggaa aacaggcaat ccaaaataaa tcgttatgtg ctctagccta aaaacaaaat
     9061 caaaggatag aaaaggaaat acatctagga tcactctttc ttccatagaa aaaatcctag
     9121 tcattattgt cagacagaac agaccaaaat acaaagttga tctctctttg ggacgacttt
     9181 taaataaaaa caaatgataa attcccataa taaaaagaga accgactgtc atcaatgcga
     9241 gataaagaga aaaattcttt ttttgctcta tatccttcca agttcctaaa aatggtttgt
     9301 tccagagtcc tgcttttaga ttttcaaaat tagatacctg tatgacaatt tctaactcat
     9361 cggaactttg aggtggaatc agaatagaat cgtattttaa aaatggaaga gattcttcgc
     9421 ggatcttccc gacttttccc atattgagtt ccaattttcc attgatgaaa atttgataac
     9481 tactacttgc atcggtcata aaaagtccga gtggtttatt gtgcggaggt aacaatactt
     9541 tcaaacgata gctggcatac ccaaatgggg gtaaggtttt tccattgaat tcctgattct
     9601 gccattcgga aggaatagaa ataattctct tttctaattc taaaatatct ttcggaacag
     9661 aactgggctt atcggattca tagagaaacc tttcccaata aaattcccat tctccttgta
     9721 agggaatcat tccatcttct gaaaaattcc aatctttgag tgaaatgctt ccataaactg
     9781 cttccgattg tattttttgg ggaaatattc tatttgtatt cctgacaaat cccaacgcaa
     9841 ggaggatgag aatcgaaatt aaataaaaga atttacccat tatgtaaaag atgtgagtta
     9901 gaacttattt gagcaagacc aaattatgca taaagcaaaa agatcgttct ttgttgcaaa
     9961 gacttataca taagaaatta catattcaat gagaatccaa tctaaatttc ttgcaaaaaa
    10021 cagaacttaa ttccaatcta ccaatgggtt tatcgttgaa ctttaactgt gacgtttaga
    10081 tttcaaaaag atcttacatt gcctttagac ttcatattag gaataggcat tttggaaaaa
    10141 gcaagacatg aatccaatgt aggttcgaat cctactatct ctttcgaggg atatagccaa
    10201 atggttaggc accagactgt taatctgaca aaaccaaaaa ccgaaagaaa aggaaattct
    10261 catgccctta tttaagagag actgcgaaag cggttaatca gaagtaaacg gcacttagat
    10321 taaaaccttg cagaaacttg gataccgatt ggatttgaaa gcttccattc cgaaaggaac
    10381 cgatgccaaa aattccttct aaaaagtttc tccgtatttt ttccgaggag ggttttcttt
    10441 tctttccata aaagtcacaa ccaattcaca gagataaaca ccgatagatt ttcatactag
    10501 ttttaaattc aacctttctt actttcttta ttctttacta agaaaagaag ttaagattta
    10561 ccaaagtcat cacatcttga taaagttgta ttcattcact attagaattt acaaattcaa
    10621 atcccaatta aaaatgaatt tctaaattcg tttttctctg atcctcagtg tctaggttag
    10681 tctatttagc catttttgat tctactgagc taagagcata ataaggtggg aatatgttta
    10741 caacaattcg aaatgatgaa agaggacttc tctttaaaga tggaaattat attaaaacac
    10801 taaagcctgg caaatatcta ttagccgcaa gtctaggaca tacactcatt cgaatggatg
    10861 taaataaagc ttttgtgaca gaaaaaaatc taaatctttt tctaaaagat cagtccttag
    10921 tcgaagaact ctcgattgta gaagtaaatg agtttgaaat atgtcttcac tatgaagaca
    10981 atctactcgc aaatgtatta aaaccaggac gttatgcatt ctggaacatc ttaaagaagc
    11041 atgaatttaa attaatcgat acaagaaatc caattccaaa tcaagaattc aatcaatctc
    11101 tattcacaaa tatcaaatta aatggatttt attatgcctt tcaagttgaa aaccatgaag
    11161 ctggacttct ctattttaat ggagtatttc aaagtatact taaaccaggt aaatactact
    11221 tctgggctgg agtcatcaca gctaacgtca taaaaataga cttacgacag caacaacttg
    11281 acatcaatgg tcaagagcta atgacagaag ataaaatcac tctccgttta aacttcacat
    11341 ctcaatacaa aattaccaat ccactcaagg tgatggaaat taaaaactac caagaacaag
    11401 tttatatttt actacaacta agccttcgtg aatatgtagg tgctttaaaa ttagatgagc
    11461 ttttacaaaa aaagcaagaa attgcagatt ttgtcctaac taaactaaaa gagaaatcca
    11521 atgattttgg aatagaattt gtatttgcag gattaaaaga tattatcctt ccaggtgaga
    11581 taaaagacat tttaaattta gtattaatcg ctgaaaagaa ggcacaagca aatataatta
    11641 ctcgtagaga agaaactgca tctactcgta gtctcttaaa cactgcaaaa ttaatggaag
    11701 aaaatccaac actttatcga ctaaaagaat tggaatatat tgagaaaatt tccgaaaaag
    11761 ttaactcaat ttcactctct tccagttcag ggattttaga acaattagcg tctatcgtta
    11821 atattaagaa ataacatgtt taaagttggt attggacaat cagatgagat cgacacactg
    11881 tgtgcgacag aaaaagcaat cgcacaagcg agaaattctc taggaagttt tattccgcaa
    11941 gcctgtattt tatttgcagg gcttgatttt gagcatgaga aaatattaga agaaatcaat
    12001 aaaacttttc cagatattaa tttgatagga tgttcttccg gtggagagat tacaagcgaa
    12061 ttcggtttta ctgatgattc gattgtaata ttgctactct attcagatga agtatctttt
    12121 tctattgggc ttgggtatga ttcagaaatg aattcagaaa tagcggcgaa taacgctttg
    12181 gagatggcac gaaaaaatct cacaaacgaa gaaaaactat gccttgtttt tactgatagt
    12241 ttcagtctct caggaaacaa gatcattcaa actctgaatc agaaagtgac aaaaggatgt
    12301 aaaattttcg gtggtgtttc agctagacca tttgtttcca acctaaagac aaagcaattt
    12361 tttaaagaca aagctctttc tcaatccgta gtagtaatgc tgattggagg acctatagag
    12421 tataaataca gtctatctca tacttggaag cctattggta gaaaagcaat tgcaacgtta
    12481 gttgaaggca aaaagttaat taaaatagat gatatgagag cactcgatta ttatcattat
    12541 tatcttggag atttttcatt tcctgctcca gaatttccac tcgcagttta tcaaaacgat
    12601 tcagaagatt tttacattcg agttcctggc ggctataaca aagaagatgg atctgtttta
    12661 tttgcaggtg aaatcccaca aggttccaca attcaactaa ctgaggcaat gagagattat
    12721 attgtagacg atttagacca agaatcgatt cgtcagaaaa aaatgaaaat taattttgag
    12781 ccagccatgg catttgcctt ttcgtgtatc gttcgaaagc aaatactagg aacaaaggta
    12841 aaagaagaaa ttcgtgtgtt aaaagaacat ttcccacctt ctttcccaat atttggattc
    12901 tatacttatg gagaaatttc tcccctagaa tcagaagaaa gtagtaattt gcacaattgc
    12961 acaatggtta ccttagtaat aggaactaaa tcaacttcca atcaaacaga aaagaaagag
    13021 gctaatcaag attatcccga agaatctcca aatttaagtg attcggaaat catagaagaa
    13081 caaaaaaatg aaattctaaa actaagaaag aaattagaaa gagaacatag ttatagagta
    13141 aacttagaaa atgtaaaaga ctttcatggt tcggttttaa aaacaatcaa tcatgaaatt
    13201 gaagcagcaa aacaagtaat tcagcaaaag aatgtagagc tagaaaaact ttacaaagct
    13261 cttgaatcag aaaaaaagaa gtcagatgat ttacttttga acatattacc aacacaagtt
    13321 gctgaagaat taaaacagaa cgattttaca aagcctgtct attatgagtc agcatctgta
    13381 ttatttaccg atttcaaagg ttttacaaaa attgcaggca aaatgagccc ttctgaacta
    13441 gttactgaat tggattttta tttttccgaa tttgatcggg tgattgaaaa atatggttta
    13501 gaaaaactaa agacgatagg cgatgcttat atgtgtgcaa gcggattacc taaaatcaat
    13561 tctaatcatg caatagactt agtaaatgca gctttagaaa ttcaagagtt tatgctaaat
    13621 ctaaaaaagg aaaaaggtga atctgtttgg gaacttagaa ttggaattaa ctcaggtcca
    13681 cttatggcag gtgtgattgg aaaaaagaaa ttctcctacg acgtatgggg agatacagta
    13741 aacatcgcat ctaggcttga gtccaatgga gaagcaggta aaataaatat ttcgcagtat
    13801 acttacgaat tgattcgaga ccactttatt tgtgaatata gaggtaaaat cgcagcaaaa
    13861 aataagggtg aaattgatat gtattttgta ttaggacgaa agtaaatgag aggaatctta
    13921 ttactccttt ttctctccta ctgcgtaacg gtagacgaga gtatcgatgg aaaagcaaag
    13981 tctattcaat tcaatgtttg cttctggaga gcagagaaat taaattccaa aattttgaat
    14041 gatactaaaa atctattagt gactctagga tctaactgtg atagtatctc atttactgaa
    14101 atcaaagcgg ataacgaagg tttgccaaat gaattggaaa atattttttc aaaattaggt
    14161 tataattata cctgtctaga tagtttccat gatgaaaaaa ctcatttaac tcacacaaag
    14221 tatataactt gtgcaaataa taacaaggta gtagaaataa agccattcga gtatgctgat
    14281 actagaagag atttttcttc cccgccaacc ttcttttttt tggaattaac aaatggaacg
    14341 aaagctaaca tcttaccatt tcatgcccat gcaggaaata aaaacgaatt aattgatttt
    14401 gaaagagttg tgaattttag ttttcagcaa ttttcagaaa ggaaaacatt tttaggagga
    14461 gatttttata cggataagaa gtatcactca gaatcctttt tgaaatcttt gctttatttc
    14521 caaattctaa aaaatttgat cctagaacct acaactatca gtggcgaaaa aaatgatatt
    14581 cttatgaccg acaaaatcac tgcactgaat tgtaagggaa atactcttat aaagtatagc
    14641 tcaagtagtc ctgtgtctaa agaattagaa ttagtatcaa accattttcc aattatcgca
    14701 caatgtaaat taaaataaat ttgacaaaat aagattgggt cttagtttct agctatgctt
    14761 ggaattcgat ttatcaaagt gcgacctact gattatgcaa ttctttataa aaaaggacag
    14821 attcgaaatg aaggagcagg tattagtttc ttttactatg ccccatcttc aaacgttgta
    14881 atagtgcctg ccgcgagttg cgattctccc tttatattta aagaaactac agtagactac
    14941 caagagatca atatccaagg acaattcaca tatcgagttc tagaacctag aaaactggct
    15001 ggacttttag attttgccgt tgatgtaaat ggtcgttata cgggagatgg ttttgaaaaa
    15061 cttcctgttc gacttatgaa tgtgattcaa gttactatcc gtgaaaaact ttctggactt
    15121 gatttaagaa cagctttaaa atctggaagt gaatttgtat cccatgtgaa atcaagacta
    15181 aaagacaatg aatctctatc tgcgttaggt gtagaagtag ttgactttgc catattgaaa
    15241 ataagcccaa ccccagaaat gtctagagca ctagaagcaa gtgcaaggga aactctactc
    15301 aaagaagcgg atgaagcaat ttacgtcaga cgaaattttg cagtagaaca agaaagaaaa
    15361 atcaaagaaa atgaattgca aacccaaatt gcaatcgaag aaaaaaatag aaaaattcgc
    15421 gaagaacaaa tgaatattga aattgcagtt caagaaaaaa aaagcttgct cgaagaagca
    15481 agaatgaact cgcaaaaagc cctccaagaa aaagaaaatg aaatagaagc acaaagatta
    15541 aaagcagaaa tccaaagaga agaggatcgt aagcttcttg tgaatcatca gtccgaaaat
    15601 ataatccaaa cctctaaagc taaagcggaa gcaattcgtc ttgagctaaa tgcattaaca
    15661 agcttaactc ctgaattttt agaaatacta gcttcaaatc aaatgaactc acagcagatc
    15721 gttagtcgcg caatgagaga gcttgcaaaa aatgcagata aaattggtaa tctaaacatt
    15781 accccagatt tattaaataa tttattagaa aataataaat aatttttatc taatttaata
    15841 ttctaatcat tctaaaacaa gaggtataaa atgtattatg ttctatttct atttataagc
    15901 ataagtgtgt ttggacagac tacgactaat ttggttaaga aagtctctat cagtaatttc
    15961 tcaaattata aaacaaatcc agattcaaaa atgcaagaga atatatataa atcactctca
    16021 aatcgattta aagaaaatgg ttatactgtt gatcaattaa cagagagctc tcttgaaaaa
    16081 aagttagcca cagctaaagc aaatggaagt agttttttaa tcgatggata ttataaaaaa
    16141 acatccggtg aaaatttaaa tatttttgtt caaatttata ataccgaaaa cggcaaactg
    16201 attgatgcat tcaatcaatc accagaatct cttccagaac taggtcaaat aacgttagat
    16261 aaatctgaaa tgacgatcac agatgataaa gcaatagaag atatgtctaa aaaagttttt
    16321 actagaatcc gtgcaaatcc aaaacgcctt gagaatcgag agaatataaa tgaatatgta
    16381 gcgcaagctc cgattggaaa tgaaatgaat atccctatcc cacaagaaga tgttcaaaaa
    16441 gcttccgagg atgtattcaa actattaagt gaagacaatt ctatttccat tgtatcaaaa
    16501 agagtcggac agtcagagtc agttgataga acatcagcta tcgtatcggt tatttctaga
    16561 tcaagaatcg ttgattcagg agctagaaat ttggctgata ttttaaaatc tgttccaggt
    16621 ctggaggttt tttatgatca attcgggttt tataaagttg cactgagggg cattcgttcg
    16681 aaatctggaa tgcttcttat gattgatggt caccgaatga ataatttcta cgatggttct
    16741 accttcatag acattcgtgc tgatgctatt gaaaaagttg aaattattag aggtcccggt
    16801 tcttctgtcc atggaacaaa tgcgttagtt ggagtaatta acgtagtaac tagagatctt
    16861 ggatcaggaa agaattcaca gggtgtcttt agcacacgtg ctggatcgtt taatacattt
    16921 gaaccaacgg ctttctattc aaggaagata ggtgagtcag attggagatt tacaacctat
    16981 gctggaagct atcaatccga tcgccaacaa aatcgaattc cttatgatga ttcttgtgct
    17041 agaaccactc ctactgcggg gcaacctttc ggagggctgt caaatgcttg tagtaatcca
    17101 ggttatactg tcttaccatt atacgcttct ccttgggtaa aaacaaatga ccaaaaaaga
    17161 caaaacaatc ttttcctcaa acttcaaaat ggaagagatg gattctatat tactggcaag
    17221 gttttaaatg aaacaagagg tcccaatatg ggggaaattg caaccgttac gccctcttct
    17281 gagctcaact tcaattttat gaatggtgcc atgggtatca ataaattgaa tattaccgaa
    17341 aagctattaa tcagcggaaa agtatatgga gattcttatg ctcgaagaga tgatatacaa
    17401 gttgaaagag aggactatcg caatcataca tttatgagcg ctagaaaaaa aatctcctat
    17461 gaatactcta ccaaagggat tgaatctgtt attcaataca atgcgctttc aagcctcgtt
    17521 gtaatgcttg gttttcaatt tgaaaaactt gctgtgaaga atttttatac gcaacaaaat
    17581 tatctatcta ctacaacagc accgcttcca ggattattgc cattcttttg ggattatgat
    17641 ggaactcaaa aaaatcaaaa cagagagatc tatacaaggt gagtatatac aagtaatttg
    17701 ggatcctctc cagtggcttt caatcacaat gggattgaga caggatagat attctgattt
    17761 cggaagaacg ttaaacccta aaggggggat cgtcattatt cctttcgaaa agatctctgc
    17821 gggaacttta attttaaaaa tcctaggtgg atcagcattt agggcgccta catttcaaga
    17881 gttatacgac agaacccagc aatatcaaat tggtggtatc ttcggaaacg ataaactgaa
    17941 accagagaca attagaacca ttgaaattgg agcagattac aatacaccct ataaaccttt
    18001 gagtatactt ttaaatggat atgcaaataa aatctataat aacatagatg gatttaatca
    18061 atcaaatgta tttcctggaa gaacagatac atatgttaat ttgagaggta tatctatgat
    18121 aggttacgaa gtggaagcaa gattgaacta ttctgctaaa aattatggtt acgcaaatgc
    18181 ctcctgggtt caagttcttg atagcggtgg aattccgcct aacgataagg ttgacacagt
    18241 tacgtttaga atcgatgttc cacaagcacg agcaaatatt ggtataaatc atgaatttac
    18301 aaagtatttc actttaaaca attcagtttg gatttcttct gagagagggt ctaactctcg
    18361 ttatccttcg gaagctcagg ataatagagc atttagaatt cctcaatatc atatttggaa
    18421 tgtaagtctg gctacaaccg atgttgtttc taagaattta aactttgcgt taaatatatt
    18481 taatctaaac aattataaac tatatgatga cataaataac gtaacatcag ccttttatcc
    18541 aaatagagtt atcccttcag gctatctctg gggtcgctat attgagttta aactaacata
    18601 ttttctaaat taaggagact actatgaata aattaatttt tttattaatt atcaatttgt
    18661 ttttttgcat tcaaaaaaaa gcacctcctg caaatgcgtt actcgctact tcggcaacta
    18721 cgaatgtttc ggtaggtgct ccaaaaagta tcggcttaga tttagtgaca aataatagat
    18781 cttggaatag aggttgcgag acaagagttg ggaatctaat tgctgattct ttcgcttgga
    18841 aggcgaatgc tgatatagga actgcaaatg gtggaaatgt tagagatgat caaaatgttc
    18901 ccacaattaa aaaaggaact attcctgata atactttatt cgggaaattt ttaatattca
    18961 aaaatcaaat ctatttaact caaatcaatg catatcgctt aaaacaagca ttggaaaatt
    19021 ctaaccgtca gttgacaact actagaattg cagcgagcac agatgataat gatgtagatg
    19081 gtccacagca tggaaattgc tattcctcag gaagtggtag tggtagactc ctctatgtta
    19141 gttccaatct ttctgttgat gtaacagtag caaatacacc aatgactgta accggttctg
    19201 ctgcctctaa taatttagca actggtgtaa caggcgcacg cattacaaga atgtttgtaa
    19261 agggagtcat gatttataat aatccaactg gaagctttac atcgggctgg gcaaacggaa
    19321 catcctcttg cacagtaaaa caaacttcat tcacaaatag tgctgcatgc aatcaatata
    19381 ctctagcgtc tgtcgacttt caggtaaatg gtggagatgg aaatttttct ttcaaccctg
    19441 atgtagctcc tag
//
LOCUS       DAOQOS010000106        19273 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_1425_length_19273_cov_6.378655, whole genome shotgun sequence.
ACCESSION   DAOQOS010000106 DAOQOS010000000
VERSION     DAOQOS010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 19273)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19273)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19273
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1425_length_19273_cov_6.378655"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..187)
                     /locus_tag="PLF66_19265"
     CDS             complement(<1..187)
                     /locus_tag="PLF66_19265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004490895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HMZ66239.1"
                     /translation="MKKNNSFLVTEHGTISTILGKDTYFNGILTFKKPLQISGEFEGE
                     IVSDGFLVISEGAVIRAN"
     gene            complement(209..430)
                     /locus_tag="PLF66_19270"
     CDS             complement(209..430)
                     /locus_tag="PLF66_19270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010575394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HMZ66240.1"
                     /translation="MITPQMKEVLVHYNQGLSLYKDRKFKEALDSFKKGLEIIPDDGP
                     TLMYIKRCKEFIETPPPADWDGVYVMKTK"
     gene            complement(445..825)
                     /gene="acpS"
                     /locus_tag="PLF66_19275"
     CDS             complement(445..825)
                     /gene="acpS"
                     /locus_tag="PLF66_19275"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HMZ66241.1"
                     /translation="MILSVGNDIVENSRIKETFDKFGEKFLERVFSKEEVEYCLSKKN
                     PIPHLSARFACKEAFIKAIELENGKALEMKEIELCGNFFGKKNLALHGKARELFIEKG
                     FNEISVSISHTDSYATAVVILYNK"
     gene            complement(822..1859)
                     /locus_tag="PLF66_19280"
     CDS             complement(822..1859)
                     /locus_tag="PLF66_19280"
                     /inference="COORDINATES: protein motif:HMM:NF019561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbbR-like domain-containing protein"
                     /protein_id="HMZ66242.1"
                     /translation="MRLFDFWQAKIGSVLLAIIFYVNLQNSKVLVKTINVPIEYPRLT
                     GNLYYSKNSEKTFQIRVEGFRDLVNYHSQFMKVVVDPSELTVGENQYEVKKIWGAPSS
                     GIKITMLGPKLNIGVDVLASKTMAVDVGFDDDLPQGFIRSSHSVKPSTITVTGPKTVV
                     EKMSKFVLPTVSLKDATESFTRTIRIPEFQKNLGLVGNIKEFQLRVNIIRDLSNIGDQ
                     IIVQLPVKCEYLDPALEVDLSAEEVSIKFSSTTKVNSIQIIQGIQASVPCNYTYDKKT
                     KKISPNSLPVLAKVRIVKSPDLKNVEILGVIPEKINIQYKIKPGIFEKEKKDGSSDEV
                     YYPEDLSEDRK"
     gene            complement(1856..2641)
                     /gene="cdaA"
                     /locus_tag="PLF66_19285"
     CDS             complement(1856..2641)
                     /gene="cdaA"
                     /locus_tag="PLF66_19285"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016544629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HMZ66243.1"
                     /translation="MLYIDYNNLSPISVTFDIIIVSYIFYKIYIMLRKTRGLQLLIGV
                     GLFYIMGLVANYFQLELLDWLIVNIKPAIIFVVIVLLQPELRRNLGELSKIRIVKLFL
                     LKPNYELDEIVEAVKMMAQDKTGSIIVIAKDISLKDIIEQSVQIDAVISSSLLLTIFK
                     KNSALHDGAVIIEQNRLASASSYLPMSNSLGNSTLGARHRSALGLAEETDAVVIVTSE
                     ETGEISICKDGEMYHPVKSFELKNQLQLLLTDKYPKKEKGGNL"
     gene            complement(2661..3476)
                     /gene="dapB"
                     /locus_tag="PLF66_19290"
     CDS             complement(2661..3476)
                     /gene="dapB"
                     /locus_tag="PLF66_19290"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016752821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HMZ66244.1"
                     /translation="MSRKGKLKVTLIGSAGRMGVSITQVLSKSEKSELFAAVEHEKSP
                     LIGKDIGIHAGLGEIKKFYTSSQEDAISEGDVIIDFSTAENSKKVLDLAVKHGKPLVI
                     GVTGLEEDFLQKIREASVSIPILQSPNMSVGVNLLFKLAEMTARVLGDDYDIEILDIH
                     HRHKKDSPSGTAMRLKEVLLSTLGRGEENVIYGRHGKEYGERDKREIAVHSMRAGEVV
                     GDHTAYFFSADEAIEISHKARDRRTFAVGAVKAAEFVSEQKIGSFHMFDVLGL"
     gene            complement(3469..4353)
                     /gene="dapA"
                     /locus_tag="PLF66_19295"
     CDS             complement(3469..4353)
                     /gene="dapA"
                     /locus_tag="PLF66_19295"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016748926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HMZ66245.1"
                     /translation="MFEGVFTAVITPFKDGKIDYTAYFKILDSQIGSGVAGIVPCGTT
                     GESPTLDYEEHLELIQKTVSYVNHKIQVIAGTGSNSTKEAIYLTEEACKAGVDGVLSV
                     NPYYNKPTQEGLFQHFNLIANASEKPVMLYNIPGRTSVNLSVDTIKRLSENPKITSIK
                     EATGDLGQMAKTIQAVGSKMTVLSGDDGLTLPLLSIGGKGVVSVISNLFPKTMARMVK
                     LFTGGEIEASRKIYYDFLSLFGLAFCETNPIPVKAGMHLLGYCENTLRLPLTPLSNSS
                     QADEFKKQIFHLRGNGYE"
     gene            4694..5890
                     /locus_tag="PLF66_19300"
     CDS             4694..5890
                     /locus_tag="PLF66_19300"
                     /inference="COORDINATES: protein motif:HMM:NF026220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HMZ66246.1"
                     /translation="MKKILLILLINFFLYCGYWNGYKKMKEEEKFGQDLVPICFLDYK
                     RNNVGIPGKSSNKILAYNNNTLYLITKRSDYSLYGYDGFVKLTKFNLIFSSKDDGISW
                     TENSLPDQTQISEFQSIYFSVDTFVTFWKTSSTATPIQISYSNDLSSWRTNATTETNS
                     SKASFFDKDNIWIYTSSKIYKFGLTTGNMNLLYTSPLAEQTAGENILDIKFVTAQIGY
                     SISKSYLKKTTDGGSTWTIVYTINNDSITDFFVLNSSSVWLRSSFPKLYYSNDGGNNF
                     TANSLGENLKSGKMIFTSSSNGMISNNNMLYTTSDSGANWKLVSFTSTTSVSDILFAT
                     ANTGYLSNQKQIFKTTDFGNTWSKQTDSADSFNYELMNPLMTSANILLIACLQVAKYS
                     GHPPDK"
     gene            5902..6987
                     /locus_tag="PLF66_19305"
     CDS             5902..6987
                     /locus_tag="PLF66_19305"
                     /inference="COORDINATES: protein motif:HMM:NF026220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HMZ66247.1"
                     /translation="MKRLLILFLLNLNCFHFSLPPPSGDVDKNIYTCLALTQLKTERK
                     GNYNLSMIKRFGDKLFVNSIRSLPNQSGVGFSDSDVYLFSNDGGLSWLKLLYPPKDRN
                     GWDRFFFSSDSIYNLRSQNLDKLSATDTNQWVTFPYLLSNNSIQFLDTYDTSLLLATN
                     LSVYRYDTNIQSLIKIFDFNLAGNISAIDFTDSLNGWIISNQKIYNTVNGGQTWNLKY
                     TFAANTGYSQVFALSNTNFYFITSNKLTYTLDNGASWLTYTFPDSQPLSNLFFYSKSI
                     GFILQNGILYKSTDQGSSWTKVFPDSTFTEKIVSHTWVTDKTGYAIGAKAIYETLDSG
                     QSWTLKNPDIDSFTYQARATPPLLCEF"
     gene            7477..9825
                     /locus_tag="PLF66_19310"
     CDS             7477..9825
                     /locus_tag="PLF66_19310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66248.1"
                     /translation="MLESRTILLILFILITNCLKVKKSPFDMTKPSGLAFGIFSIFFS
                     NSDVTLSGTIEGKALQDSITLTSGAETKVFQNTNSFGFTYPRNQNFSIDIVTNPSGLS
                     CSMLNNKGYSSSNISNILVICEQSKPSLTYPTNGKNWGDYIQRDYSKDILSQTDSPCD
                     PNSASNKNYFSCIHGAEILQVELKGKTSCDGITASDNANQTGGALNWNCIVKNSKVIV
                     YSTGFREGDLGALNTNPPPGLSDLIDWTSGNKFKSITLTVKDKDIVYLQTTPSTWWNN
                     SFVLNPSCATSLVQGAQEINTIYTFSSSSDYNVSQASPCQMDSDKTSFVIDPKIRLTN
                     SISGGTLLLINNRNFNWIEGYFHGLNKNTNVFLSDSKFLVARNLTAAEITGGGGAGFN
                     LRLSNMSLSLFKNVIIANSLFVNPGIGVNVESSSMKNIFHSMKVFNNSATGINLNSAQ
                     SNLFIHLVTSANNGVGLNIPATSDNNFILNSTHAFNATTGITTNANNTLIMNPLITNN
                     NSTTNFGFDTSAANLNLTVLNIGLFNNNSNANSQIQSSGNGTTNYKKYLDQFKLSFLN
                     CTGGGTSFDGISATNCNPIGQSDFTVGALINFSTSNPFVSIKASSDSLNSSVTASGLP
                     LSSITNFSRVQSFYRVYGLYSLVNFPNFNLIGRCTTQCTLYDFGFSLTDSSGVRNSNP
                     CPQNLSRPTLSFLADTILRNSIEILGDWKGDDDGLCESNEDCIYSPNIGVYQGHGSLL
                     SASTSNTSYPNTVNQCQDITTATGVDGAVTNIKLYKYEKNGY"
     gene            complement(9965..11182)
                     /locus_tag="PLF66_19315"
     CDS             complement(9965..11182)
                     /locus_tag="PLF66_19315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 family protein"
                     /protein_id="HMZ66249.1"
                     /translation="MWEDFQQHLWLYLSMPIVAALIGYITKLVAIRMMFEPLKFIGIK
                     PFLGWQGIIPRRAEKMAAIAADTLTARLISTRELYNKIDPAEIIKNLEEPLIRDIDII
                     ADEVIGQMQPELWESLPTIVRDQLIGNLRKEIPGVIKSLMADMDRDLDTLFDIKTMVV
                     MIMSKDKALINRIFREVGAPEFKFIANSGIYFGFLIGLIQAFTWMLTHSPLIMPVFGL
                     FTGWFTDWLALKMIFYPKKPTKYLGLVTWQGLFIKRRQEVAAEYGALIAQEILTPKNI
                     LEFILSGPLSDRLYEMVIRIIKKTIDSQVGLAKPLVIMTIGGQKYQDMKEKAAMKIIE
                     RLPETLKNIETYVGNALDIKNTIVSKMKQLTEEEFESVLRPAFQQDEWILITVGAILG
                     FLVGEMQVFLMTH"
     gene            11359..11592
                     /locus_tag="PLF66_19320"
     CDS             11359..11592
                     /locus_tag="PLF66_19320"
                     /inference="COORDINATES: protein motif:HMM:NF015630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0175 family protein"
                     /protein_id="HMZ66250.1"
                     /translation="MAVIAPEILNQTGMTEKEVLTEISVLFFQMNKLTLGKAANLCGL
                     HPFQFQKILASRKIPIHYNQEDLKQDMIHLQKL"
     gene            11589..12077
                     /locus_tag="PLF66_19325"
     CDS             11589..12077
                     /locus_tag="PLF66_19325"
                     /inference="COORDINATES: protein motif:HMM:NF023276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3368 domain-containing protein"
                     /protein_id="HMZ66251.1"
                     /translation="MIVVSDTSPISNLIVIGNLNILITLFQDIYIPPKVFAEILRLEE
                     FNINVSEIKNSNSIKIKSASNAQLIQDLKETLNEGESEAIALAKELEADYILIDERKG
                     RKIAEQMGIKSIGLLGILLKAKEKRVFERIAPILNALREEAGFWISDILYHDILMQAN
                     EI"
     gene            complement(12086..12733)
                     /locus_tag="PLF66_19330"
     CDS             complement(12086..12733)
                     /locus_tag="PLF66_19330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012389431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HMZ66252.1"
                     /translation="MTAKKKNKKKQVKAGRPFKKSGFTVREDLILAGTELLKTSSLEN
                     LSLRQVSAKAGVSHVATYHHFKNKNALLAAIAEKGFEKYFSDYQKELEKTKNDFLGKY
                     KALGITYVRFIMSNRQFARIMFGGSGVDIKSDPKLAAVSRKTYRQLHEIIRLGQKIGA
                     IGEGNARNKTLASWAMIHGIAMLFLEGRIKTKDNQKEMEDLIESVTEYAYLGMRK"
     gene            13125..15119
                     /locus_tag="PLF66_19335"
     CDS             13125..15119
                     /locus_tag="PLF66_19335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015679111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HMZ66253.1"
                     /translation="MNTAFQPIQIGNYTLPNRFIMGSMHLGIEGEIGTAERMAAFYGK
                     RFEGGVGLIVTGGVSVNEEGKGSKSFFNIQIESHANELKKMNDILKGKGVMCAQLFHA
                     GRYAFDRSCVAPSAIRAPINRFIPRALTEEECWKTIEDFGKAAKLARESGFGAVEIMG
                     SEGYLLNQFFSPVTNQRDDFFGGDNKRRMNMSIEVLKSVRKNLPEGFPIIFRMSGIDL
                     IPGNPTFEEVVTLAEALVSEKVSALNIGIGWHESRIPTISQLVPRGAWARTASKIKER
                     ISGVPIIASNRVNDPSTMEKIFSEKQADIISMARPFLADASIVNKIKSGMGNRVNTCV
                     ACNQACLDHAFQEKSVSCIVNPEAVNEIKYSGKTNVSPKKVLVIGSGPGGLEAARASA
                     SLGHKVILLEKGSELGGQFQLASHIPGKSEFKETIRYFKNELPALGVEIRYNTNADLK
                     LIEEINPDAIIFATGVKPREVEIKGLENLPHGNYTEYLTGKFVPGKKVAVIGGGGIGV
                     DVAHKLTEETDPTFESYDEKYNIQSYTNVAIQKHKAKKEVAVFRRNGKHGAGLGPTTF
                     WALKQELEFAGVEFFHGLNYKEVTKDGITVTLKNGEEFTYPCDSIVLCVGQEKESSLY
                     EEFTSRYPQRKVFLIGGAKEAKAIDAKKAFLDGLEAAYNL"
     gene            15128..16858
                     /locus_tag="PLF66_19340"
     CDS             15128..16858
                     /locus_tag="PLF66_19340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015680460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HMZ66254.1"
                     /translation="MNFFSEEEDLKLIFDSLIDWKPIVEEAEGDFLDHKYYLKTKDPR
                     FEMAPSDLESAIDFYKSCLTSLGEFFGEEVFPFAKEIDHKGLKFENGKVIFPEELIQV
                     YEKLKSTGLLPYSIARSGGGLGLPATIGALFAMIMARSDVSICMTVTLLNLAQIINRY
                     GTKEQNEKYTVPAASGESLFAMALTEPDYGSDLNNVRTVATKMEDGSYRINGTKRFIS
                     QGCGLGSHPAFLLTLARTGTQKGARGLSVFLVKSSDVQVAGIEKKLGIHASPTCEIVY
                     ENSYAEILGEEGLGLTRYTTGMTNFMRLASAAGGPGGGAAALYECRKYAKERMQFGKP
                     IEEIPAVSEMLNKIERETNAMRLITLETARVIDLYQHTQIRLEKSGKEDREIRKNERV
                     QRYSALASILTPVAKYYCSEEGNKLAYLAIQIHGGVGYTEDFNVAKLYRDSRINSIYE
                     GTSQIHVRIATGAIFAGMSEEGLFRKYLKELVANIDLPSDYLKEHTELLEKAILSFKQ
                     IPIDTEKERVAENLMILVARYFGSILYENSVQKLKGKPQFERWKKDARNYAIESTAIA
                     HSCLFRIQNL"
     gene            17285..18250
                     /locus_tag="PLF66_19345"
     CDS             17285..18250
                     /locus_tag="PLF66_19345"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66255.1"
                     /translation="MLTFISSTILLRGFYHSKFFSHLDVPNHRSMHDYPTKKSAGLVF
                     MGLFMISLFYVQIYSKDGSFSLLSQTLPGLMVCLLIGFLDDRYNFSSRRKLFFEILFF
                     IPYVYLFFPDYQVFGFTLPLPHIVFSIVLAGYIIFVTNLCNFMDGLDLYLSLSILSSI
                     GIYFSILHPNPNQAITVLLLVTSILSFLYFNFPPARLFMGDTGSLPLGYMVAILPFLN
                     GNEIPDLSYGFLLIPVFWIDGVFTILIRLKKKENIFRAHREHLYQKITLQKLDKRFTT
                     FIFSLWNLLTIPVYFFLKDSIPLMGIISIILFLNGFLYTILIRKL"
     gene            18385..19008
                     /locus_tag="PLF66_19350"
     CDS             18385..19008
                     /locus_tag="PLF66_19350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66256.1"
                     /translation="MKILERVLSKIFSLALLWEGTKKKVTVSEFLEREIILEHPFRVI
                     SMFLFTLLLFILLGLIGSYYTINHILQILSNLIHKNFDFSSIVILLIAVPLSSFGIIV
                     CFSATCKYALEIQALILLLYRSKEVYGNVEKIEYSIRTGKNWKTITKHCTVSFLSPLG
                     EKITMIGTNSDGYFHDILEQEIEENRQIKILLDPKNPRIARMIHKES"
BASE COUNT         6357 a         3466 c         3172 g         6278 t
ORIGIN      
        1 tatttgctcg aatcaccgca ccttcactaa tcaccaaaaa tccatctgaa acaatttcgc
       61 cttcaaattc accagaaatt tgaagaggtt ttttgaatgt cagtattcca ttaaaatatg
      121 tatctttccc caatattgtg gaaattgttc catgctctgt aactaaaaaa gaattatttt
      181 tcttcatcct atctacaaca cttataaatt attttgtctt catcacatac accccatccc
      241 aatcagccgg aggtggtgtt tcaataaact ctttgcatct ctttatatac atcaaggtgg
      301 gaccatcatc aggaataatt tctaaacctt ttttaaaact atcaagtgct tctttaaact
      361 ttctatcttt gtagagagaa agcccctgat tgtagtgaac taaaacctct ttcatctgtg
      421 gtgtgatcat acagtcttcc ctttttattt attataaaga attacaaccg cagttgcata
      481 cgaatctgtg tgactaatcg acacagaaat ctcattaaat cccttctcaa tgaataattc
      541 tcttgccttt ccatgcaacg caagattttt ttttccgaaa aaatttccac ataattcaat
      601 ctctttcatt tccaatgctt ttccattttc taactcaatc gcctttataa atgcctcttt
      661 gcaagcaaac ctagcactta gatggggaat tggattcttt ttcgataaac aatactctac
      721 ctcttctttc gaaaagactc tttctaaaaa tttttcacca aatttatcaa aggtttcttt
      781 gattctgctg ttttctacaa tgtcatttcc aaccgagaga atcattttct atcctcagac
      841 aaatcttctg gataatacac ctcatctgat gatccatctt tcttttcttt ttcgaatata
      901 cctggtttaa ttttatattg aatattgatt ttttctggaa ttacacctaa tatttcaaca
      961 ttctttaagt cgggagattt tacaattcta acttttgcaa ggaccggcaa tgagtttgga
     1021 gaaatctttt tggtcttttt atcatatgta taattacaag gaacacttgc ttgaatacct
     1081 tggataatct gaatgctatt cactttggtg gtagaggaaa acttaataga aacctcttct
     1141 gcagataaat ccacttctaa agcaggatct agatactcac atttaacggg caattgaaca
     1201 ataatctggt ctccaatatt ggaaaggtct cgaatgatat ttactctgag ttgaaattct
     1261 tttatatttc ccacaagccc caaatttttt tgaaattctg gaatccgaat ggttcttgta
     1321 aaagattcag ttgcatcctt caaagataca gttggtaaaa caaactttga cattttttca
     1381 acgaccgttt taggtcctgt cacagtaata gtagaaggct ttacagaatg agaggagcgg
     1441 ataaaccctt gtggcaaatc atcatcaaaa ccaacgtcaa ccgccatcgt cttagaagct
     1501 aaaacatcta ctccaatatt tagttttggt ccaagcattg tgatcttaat tccactagaa
     1561 ggtgctcccc aaattttctt cacctcgtat tggttttctc cgaccgttag ttcacttgga
     1621 tctacaacca ccttcataaa ttgggaatga taattgacta aatctctaaa tccttccact
     1681 cgaatctgaa aggtcttttc gctatttttt gagtagtata aatttccagt gaggcgagga
     1741 tattcaattg gaacattaat cgtctttact aatacttttg agttttgcaa attcacatag
     1801 aaaataattg caagtaaaac agaacctatc tttgcttgcc agaaatcaaa gagtctcaca
     1861 gatttcctcc cttttctttc ttgggatatt tatctgtgag taaaagttga agttgatttt
     1921 ttaattcaaa ggattttaca ggatgataca tttctccatc tttgcaaata gaaatttcac
     1981 ctgtctcttc tgaggtaacg atgactactg catctgtctc ttctgcaaga ccaagtgcag
     2041 aacgatgtct tgctcctaac gtagaatttc caagactatt actcatagga agataagaag
     2101 aggctgaagc gagtctattt tgctcgatga ttaccgctcc atcatgaaga gcagaattct
     2161 ttttgaatat tgtaagcaaa aggctagaag aaattactgc atcaatttga acggactgct
     2221 caatgatatc ttttaagcta atatcttttg caatcactat gatagaacca gttttatcct
     2281 gtgccatcat ctttactgct tctacaattt catctaactc gtaattgggt ttgagtaaaa
     2341 ataattttac gatccgaatt ttggaaagtt ctcctagatt tcgtcttagc tctggctgta
     2401 ataaaacgat cactacaaag ataatagcag gcttgatatt tacaatcaac caatcaagta
     2461 attctaattg gaaataattt gctacaagtc ccattatata gaataaacct acaccaatta
     2521 acaactgtaa gcctctcgtc ttacgaagca taatgtagat tttataaaat atatagctaa
     2581 ctatgattat atcaaatgtc accgagatag gagacaaatt attataatca atatatagca
     2641 gggacttgag ataatccaat ttataatcct aaaacatcaa acatatgaaa agaacctatt
     2701 ttttgttctg agacaaactc agctgctttc acagcgccaa ctgcaaaggt tcttctatct
     2761 cttgctttat gagaaatttc aatcgcttca tcggcagaga aaaaataagc tgtatgatct
     2821 ccgaccactt ctcctgctct catactgtga actgcaattt ctcttttgtc tctctcgccg
     2881 tattcttttc catgtctgcc atagattaca ttttcttccc ctctgcctag agtagaaagt
     2941 aatacttctt ttaaacgcat tgcggttccg gatggggaat cttttttatg tctgtgatga
     3001 atatctaaaa tttcaatatc gtaatcatct cccaaaactc ttgcagtcat ttcggctaac
     3061 ttaaaaagta aatttactcc aacagacata tttggagatt gaaggattgg aatactaaca
     3121 gatgcctctc gaatcttttg taaaaaatct tcctctaatc cagtgacacc gataacaagc
     3181 ggcttaccat gtttaaccgc aagatctaaa actttcttag agttttcagc tgtgctaaaa
     3241 tcaatgatca catctccctc agatatcgca tcttcttggc ttgatgtgta aaattttttt
     3301 atttctccaa gacctgcatg aattccaatg tcttttccaa tgagaggaga tttttcatgt
     3361 tcaacagctg caaagagttc tgatttttca ctcttagaaa gcacttgagt aatagaaact
     3421 cccattctac cagctgaacc aatcagtgtt actttcaatt taccttttct actcatagcc
     3481 atttcctctt aaatgaaata tttgcttttt aaattcatct gcttgtgaac tattagaaag
     3541 tggagtgagc ggcaaacgca aagtattctc acagtaaccg agtaaatgca ttcccgcttt
     3601 tacagggatc ggattggtct cacaaaaagc caaaccaaag agagataaaa agtcataata
     3661 aatttttcta gaagcctcta tttcaccacc cgtaaaaagt tttaccatac gagccatagt
     3721 ctttggaaat agattggaaa taacagaaac aacacctttg cctccaatgg aaagaagggg
     3781 aagagtgagt ccatcatctc cagaaagaac ggtcatcttt gaaccaactg cttggattgt
     3841 tttagccatt tgacctagat cgcctgtcgc ttctttgatt gaagtaatct ttggattctc
     3901 agaaagtcgt ttgatcgtat cgacagatag attcacactg gttcgacccg gaatattata
     3961 aagcataaca ggtttctctg aggcatttgc aatcaggttg aaatgttgaa aaagtccttc
     4021 ttgggtcggc ttgttataat agggattcac agaaagaact ccatccacac cagccttgca
     4081 agcttcttcg gtcaaataaa tcgcttcctt agtggaattg gaaccggttc cagctattac
     4141 ttgaatctta tgattcacat agctaaccgt tttttgaatg agttccaaat gctcctcata
     4201 gtcgagggta ggagattctc cagtagttcc acaggggact atccctgcta ctccagagcc
     4261 aatttgagaa tctaagattt taaaataggc agtataatca atttttccat ctttaaatgg
     4321 tgtgataact gcagtaaata cgccttcaaa catagaaata gagttttaag aacgaatgtt
     4381 tttggcaatt aaaaactgaa tttattcaga taaagagaga gctctgcttt gaacaggaaa
     4441 aagaattcct gagtttattt cctatgatta cgcagtaaaa ataaaatatt actagtatta
     4501 ggtaggatct acttcaaact gtggtatcaa tcagttatgt gcatacctag atatgaaacg
     4561 aattcctaaa agatgttatg aaaactaact cgactcaact tcttttttaa aaccattctt
     4621 ctaacatata acgatttgag ttcaaattcc taaaagaaag gaagaaagcc ttaaggtaaa
     4681 ggggcaagca aacatgaaaa agatactgtt gatacttttg ataaactttt tcctttattg
     4741 tggatattgg aatggttata agaaaatgaa agaggaagaa aagttcggtc aggatttagt
     4801 tccaatctgt tttcttgatt ataaaagaaa taacgttggt atacctggca aaagttctaa
     4861 taaaatttta gcttataaca ataacactct ctatttaatt accaaaagat cagattatag
     4921 cctctatgga tatgatggtt tcgtaaaatt aaccaaattc aatttaattt tttcctctaa
     4981 agatgatgga atttcttgga cagaaaattc tttgccagat caaacacaaa tctcagagtt
     5041 tcaaagtatt tacttttcag tagatacatt cgtgactttc tggaaaacaa gctctaccgc
     5101 tactccaata caaatatctt attctaatga tctaagtagt tggagaacaa atgcgacaac
     5161 cgaaacaaat tctagcaaag ctagtttttt tgataaggat aatatctgga tctatactag
     5221 ttccaaaatt tataaatttg gattaacaac aggtaatatg aatctgctat atacttcccc
     5281 actagcagaa caaacagcgg gagaaaatat tctagatata aaatttgtta cagcacaaat
     5341 tggctactct atatcaaaat cctatctaaa aaaaacaacg gatggtggct ctacatggac
     5401 tatcgtatat acaataaata atgatagtat tactgatttt tttgttttaa actcttcctc
     5461 tgtttggtta cgatctagct ttcctaaact ctattattca aatgatgggg gaaataattt
     5521 tactgcaaat agtttaggag aaaatttgaa atctggaaaa atgattttta ccagttcaag
     5581 caatggaatg atttctaaca acaatatgct ttatacaaca tcagactcag gagcaaattg
     5641 gaaattagta tcctttacct caacaacctc tgtttcagat attttatttg caacggctaa
     5701 tactggttat ctttcgaatc aaaagcaaat ttttaaaaca acggactttg gaaatacctg
     5761 gtcaaaacaa actgattctg ccgactcttt caattatgaa ttaatgaatc ccttaatgac
     5821 cagtgcaaac atattgctca tcgcttgttt gcaagttgca aaatattcag gacacccacc
     5881 tgacaaataa taagaggaaa tatgaaaaga ttattgatac tatttttatt aaacttaaat
     5941 tgcttccatt ttagtttgcc tcctccctca ggcgatgtag ataaaaacat ctacacctgt
     6001 cttgctctaa cgcaactaaa gacagaaagg aaaggaaatt ataatttatc tatgataaaa
     6061 agatttggtg ataaattatt tgtgaattca atacgctccc ttcccaatca atcaggagtt
     6121 gggttctctg attctgatgt atatttattt tcgaatgatg gtggtctttc ttggttaaaa
     6181 ctactatatc ctccgaagga tcgtaatggt tgggatagat tttttttttc gagcgattcc
     6241 atctacaatt tacgctctca aaatttagac aaattatcag ctactgatac gaatcaatgg
     6301 gtaacgtttc cctatctttt atctaataat agtattcaat ttctagatac atacgatact
     6361 agccttctat tagcgaccaa tctttctgtt tacagatatg atacaaatat tcagagtctg
     6421 atcaaaattt ttgattttaa tttggcagga aatatttcag ctatagattt tacagattct
     6481 ctcaatggat ggatcatttc gaatcagaaa atttataaca cagtaaatgg aggccaaaca
     6541 tggaatttaa agtatacctt tgcggcaaat accggttatt cacaagtttt tgctttatca
     6601 aatactaatt tttattttat tactagcaat aaattaactt atactttaga caatggagcc
     6661 tcttggctaa cgtatacatt tccagattca caacctttga gcaatctttt cttttattca
     6721 aaatcgattg gattcatatt acagaatgga attctatata aatcaacaga tcaaggatct
     6781 tcctggacaa aagtatttcc cgattctact tttacagaaa agatcgttag tcacacatgg
     6841 gtaacagata aaaccggtta tgcaatagga gcaaaggcaa tctatgaaac tttagattca
     6901 ggacaaagct ggactttaaa aaatcctgac attgactctt tcacatatca ggcaagagca
     6961 acaccaccac tcctttgcga attttgaaaa tttctaaaag tagaaataca ttctctctaa
     7021 aatatttacg cgaatactat tgatttaatc taaattcttt ctagttcaat ccctaattcg
     7081 tcactagcgc aaccccctaa ttattatata aaggagtcat taaaatacaa agagtctttt
     7141 cttttgctaa ctcatttcaa agtagagact aataaatatt agctacaaaa ttaattattt
     7201 acaaaaaaga ctaaaaacat acactctgca gtgactgaat tataaaaggg attttaaaaa
     7261 aatgtaattt agtcttcgaa aattttattc tttgagttgt taataagaaa agaaggcagt
     7321 aaatatatat ttcaaataga taatatctga taataagaaa taaactcaaa ttttctattt
     7381 atcaagataa atcgttactc gaaagagtat atttacttcg tttgtattca atgtatttaa
     7441 attagtgaaa ggatatctct cattcaagaa taatttatgt tagaatctag gactatacta
     7501 ctcatactat tcatattaat tacaaattgt ctcaaagtaa aaaaatcccc ttttgatatg
     7561 acaaagcctt ctggtcttgc ttttggaatt ttttccattt ttttttctaa ctcagatgta
     7621 acgttaagcg gaacaattga aggaaaagca ttacaagaca gtattacctt gacaagtgga
     7681 gcggaaacaa aagtatttca aaacacaaat tcttttggtt ttacctatcc gcgaaaccaa
     7741 aattttagta ttgatatagt tacaaacccc tctggacttt cttgctccat gctaaacaat
     7801 aaagggtata gtagttcaaa tattagtaat attcttgtga tttgtgaaca atcaaaacca
     7861 agcctcacct atcctaccaa tggaaaaaac tggggagatt atattcaaag agattactct
     7921 aaggacattc tttcccaaac agacagcccc tgtgatccca attcagcttc aaataaaaat
     7981 tatttttcct gtatccatgg agcagaaatt ctccaagtag aattaaaagg aaaaacgagt
     8041 tgtgatggaa tcacagcaag cgataatgca aatcaaacag gaggcgcatt gaattggaat
     8101 tgtatagtta aaaacagtaa agtcattgtt tattcaacag gttttagaga aggagatttg
     8161 ggtgcgttaa acacaaatcc acctcccggt ctttcggact taatagattg gacatcagga
     8221 aataaattta aatcaatcac tcttacagta aaagataaag acatagtcta tctccaaaca
     8281 actccttcga cttggtggaa taattctttt gtattaaatc caagctgtgc aacatcactc
     8341 gttcagggcg cacaagaaat aaataccatt tatacttttt cttctagttc tgattacaac
     8401 gtatcgcaag cttctccctg tcaaatggat tcagataaaa catcgtttgt gattgatcca
     8461 aagattagat tgactaattc aattagcgga ggaactcttc ttctcattaa caatagaaat
     8521 ttcaattgga tagaaggtta ttttcatgga ctcaacaaaa acacaaatgt ctttttgtct
     8581 gattccaaat ttttagtagc tagaaatctt acagcagccg aaattactgg aggcggtggt
     8641 gcgggattca atcttcgctt atctaatatg agcttgtctc tatttaaaaa tgtgataatc
     8701 gcaaattctc tctttgtaaa tccgggtatt ggagttaacg ttgaatcttc ttctatgaaa
     8761 aatatttttc attctatgaa agtttttaat aactctgcta caggtataaa tctaaattcc
     8821 gcccaatcta atttatttat tcatctagtc acaagtgcaa acaatggagt cggactaaat
     8881 attcctgcaa catcggataa taattttata ctcaactcaa cacatgcttt caatgcaact
     8941 actggtatca caacaaatgc aaacaatact ctcataatga acccactgat cacaaataat
     9001 aactctacta ctaattttgg ttttgatacc tcagcggcta atctaaattt aactgttttg
     9061 aacataggat tatttaataa caattcgaat gccaattcac aaattcaatc ctctgggaat
     9121 ggaacaacaa actacaaaaa atatttggat caatttaaat tgtcttttct caactgcaca
     9181 ggaggtggaa ctagttttga tggaatttct gccacaaatt gtaatccaat tggtcaatcc
     9241 gatttcactg ttggcgccct aatcaatttt tcaacttcga atccattcgt ttccattaaa
     9301 gctagctcag attccctgaa ttcgtcagtg actgcgagtg gtcttccgtt atcttccatt
     9361 actaattttt ctagagtgca aagtttttat agggtatatg gtctttatag tctagtaaat
     9421 ttcccaaatt tcaatctaat tggtagatgc acaactcaat gtacattgta tgattttggt
     9481 ttttctctaa ccgatagttc cggagttaga aatagtaatc cttgtcctca aaatctttct
     9541 cgccctactt tgagtttttt agctgataca attcttagaa attcgataga gattttaggg
     9601 gactggaaag gcgatgatga tggtctttgt gagtcaaatg aagattgtat ttattctccc
     9661 aatataggcg tctatcaagg acatggaagt ttactcagtg cgagcacaag caacacttcc
     9721 tatccaaaca cagtaaatca atgccaagat attacgactg caacaggagt ggatggggct
     9781 gttacaaata taaaattata taaatatgag aaaaatgggt attaacctcc attttcccaa
     9841 aagctctttt acaaatttta tagaagattt ttattcaaag cgagttttag tcgaaaacca
     9901 aaatcaatga atcctataaa aagatttttc ctctttatag gatttaatcc tagcatctga
     9961 agaatcaatg cgtcatgagg aatacttgca tttccccaac gaggaaacct aatatagcac
    10021 caactgtaat tagaatccat tcatcttgtt gaaaagcagg tcttaatacg ctttcaaatt
    10081 cctcttctgt aagttgtttc attttactaa cgattgtgtt tttaatgtct aatgcatttc
    10141 ctacatatgt ttcaatattc ttgagagttt ccgggagacg ttctataatc ttcatggcag
    10201 ctttttcttt catgtcttgg tatttctgtc caccgattgt cattatcaca agcggtttag
    10261 ctaaacctac ttggctatca attgtttttt taatgatacg aattaccatt tcataaagtc
    10321 gatccgagag tggcccggaa agaataaatt ctaaaatatt ctttggagtt aaaatttctt
    10381 gtgcaatcag tgctccatac tcagccgcta cttcttgtct tcgtttaata aaaagtccct
    10441 gccaagtcac aagccctaaa tacttagttg gcttttttgg ataaaaaatc atttttagag
    10501 cgagccaatc tgtaaaccat cctgtaaaaa gaccaaagac aggcatgatg agtggagaat
    10561 gcgttagcat ccatgtaaaa gcttgaatta agccgattaa aaaaccaaaa taaattccag
    10621 aatttgcgat gaatttaaat tcaggggcac ctacttccct aaagattcgg ttgatcagag
    10681 ctttatcttt gctcatgatc atcactacca tggttttaat atcaaataga gtatctaaat
    10741 ctctatccat atcagccatt aaacttttaa tcacgcctgg aatttccttt cttaggtttc
    10801 caattaattg atctcgaacg atagtcggta gagactccca aagttcaggt tgcatttgac
    10861 caatgacttc atctgcaatg atatcaatat cgcgaatgag tggttcctct aagttcttta
    10921 taatttcagc aggatcgatt ttattataga gctcacgggt agaaatcaat cttgcagtca
    10981 aagtgtctgc tgcaattgcc gccatttttt ctgctctcct aggaataatc ccttgccagc
    11041 ctaaaaatgg ttttatacca ataaatttca atggctcaaa catcattcga attgccacaa
    11101 gtttagtaat ataaccgatc aaagccgcaa cgatcggcat tgagagatag agccagagat
    11161 gctgttggaa atcttcccac ataatagagt ccttatttgt tttttaggat ttgctttgga
    11221 ctgcctttga gtcgcaatag aaagcacaaa cgaatttcaa actacttctg gctaaactat
    11281 attgtcaaat gatttactca aaattaataa taaattccgc ttgacttacc atttttaggt
    11341 ttgtaaaaag gaactattat ggcagtgatt gcacccgaaa ttttaaatca aactggtatg
    11401 actgaaaaag aagtcttaac agaaatctct gtattatttt tccagatgaa taaattgact
    11461 ttgggaaaag cagcaaactt gtgcggtcta catccattcc aatttcaaaa aattctagca
    11521 agtagaaaaa ttcccatcca ttacaaccaa gaagatttga aacaggatat gattcacttg
    11581 cagaaattat gatcgttgtt tcagatacct cccctattag taatttaata gtaattggta
    11641 atctgaatat attgataacg ctctttcaag atatttacat tcctccaaaa gtttttgcag
    11701 aaattttaag attagaagaa tttaatatca atgtttcaga aataaaaaat tctaattcta
    11761 ttaaaataaa aagtgcatcc aatgcgcaat tgattcagga cttaaaagaa acgttaaatg
    11821 aaggagagtc agaagcgatt gcacttgcga aagaactgga agcagattat attttgatag
    11881 atgagcgaaa aggacgtaaa atagcagaac aaatgggaat taaatcaatt ggattacttg
    11941 gtattttact aaaagcaaaa gaaaagcgtg tctttgaaag aattgctcca atactcaatg
    12001 cacttagaga agaggcgggt ttctggatca gtgatatctt ataccatgat atattgatgc
    12061 aagcgaatga aatttaaatg aatttctatt ttctcattcc taaataagca tactctgtca
    12121 ctgactcaat caaatcttcc atttcttttt gattgtcttt tgttttaatt cttccttcta
    12181 aaaataacat tgcaattcca tgaatcattg cccaagaggc aagtgtctta ttacgagcat
    12241 ttccttcgcc tatcgctcct attttttgtc cgagtcgaat gatctcgtga agttgtctat
    12301 aggtctttct tgaaacagca gctagttttg gatcgctttt tatatctaca cccgatccac
    12361 cgaacatgat acgagcaaat tgacggttac tcatgataaa ccgcacatag gtgataccga
    12421 gtgccttgta tttccctaga aaatcattct ttgttttctc taattctttt tgataatcag
    12481 aaaaatactt ttcaaaaccc ttttctgcaa tagcggctag gagtgcattt ttatttttga
    12541 aatgatgata ggtggcaaca tgactcactc ctgcctttgc agaaacttgt ctaagagaaa
    12601 gattttcaag ggatgaagtc tttaaaagtt ctgtgcctgc taagattaaa tcctctctca
    12661 cggtaaagcc acttttctta aagggtctac cagctttcac ttgtttcttt ttgttttttt
    12721 tctttgctgt cattgattca atcaccacac attactacta tcgcaccttt taagccaaat
    12781 agaggagatc cgtctgcaca ctcccctaat tttattaatc catttgattg cctgaatagt
    12841 ttcaaaaaat gcaattcttc tttttttgta tgatttgtta ctaacctaca aattcatttt
    12901 atcttgcata gaattatttt accactggta atttaattag ttgacttatt ttcagaatat
    12961 attaccactg gtaaattata ttagattcga tgtaagaaat ttctcatcgc caataaatac
    13021 agatcgcatt gataagattg attgttgcaa aattcttcat tttatcggta tcctcggtgt
    13081 ttatcggtgg tattctttaa aatcttctaa actaggagtt aaatatgaac acagcgtttc
    13141 aaccaatcca aatcggaaac tatactctcc ccaatcgatt tatcatggga tctatgcatt
    13201 taggcatcga aggagaaatc ggaacagcag agagaatggc tgccttttac ggaaaacgtt
    13261 ttgagggcgg agtcggctta attgttaccg gtggagtcag tgtaaatgaa gaagggaaag
    13321 gttcaaagag tttttttaat attcaaattg agtcccatgc aaatgaactc aaaaaaatga
    13381 atgatatcct aaaagggaaa ggtgtgatgt gtgcccaact ctttcatgca ggacgttatg
    13441 catttgatag aagctgtgtc gctccttctg cgattagagc tcccatcaat cgttttattc
    13501 cacgagctct cacagaagaa gaatgctgga agacaatcga agattttgga aaagctgcaa
    13561 aactcgcaag agaatctgga tttggtgctg tagaaattat gggaagtgaa ggctatttac
    13621 tgaatcaatt tttctcccct gtcacaaatc agagggatga tttcttcggc ggagacaata
    13681 aaagaagaat gaatatgtcc attgaagttt taaaatcagt tcgaaaaaat ctacccgaag
    13741 gatttccgat tatttttaga atgtcaggaa ttgatttaat cccaggcaat cctaccttcg
    13801 aagaagttgt aacgcttgca gaagcgttag tttctgaaaa agtatctgct ttaaatattg
    13861 gaatcggttg gcatgaatct agaatcccaa ccattagcca acttgttccg agaggagctt
    13921 gggcaagaac tgcaagtaaa atcaaagaga gaatttcagg agttccgatc attgcatcca
    13981 atcgagtcaa tgatccaagc accatggaaa aaatcttttc tgaaaaacaa gcagacatta
    14041 tttctatggc aagacctttc ttagcagatg cgtctatcgt caataaaatc aaatctggaa
    14101 tgggaaaccg tgtcaatacc tgtgttgctt gtaaccaagc gtgtttagat catgcattcc
    14161 aagaaaaatc tgtttcttgt attgtgaacc cagaagctgt aaacgaaatc aagtattcgg
    14221 ggaaaacaaa tgttagtccc aaaaaagttt tagtcattgg atccggtcct ggaggactcg
    14281 aagcagcaag agcaagtgct tcccttggtc ataaagtaat tcttttagaa aaaggaagtg
    14341 aactcggggg acaatttcaa ttagcctccc atatacctgg gaaatctgaa tttaaagaaa
    14401 caattcgcta ttttaaaaat gaacttccag cactcggtgt agaaattcgt tataatacaa
    14461 atgcagattt aaagctaatt gaggaaatca atccagatgc gattattttt gcgacaggcg
    14521 taaaacctag agaagtagaa atcaaaggat tagagaattt acctcatggg aattacacag
    14581 aatacctaac tggaaaattt gttccaggaa agaaagttgc ggtgattggt ggtggaggaa
    14641 ttggagtaga cgttgcgcat aaactcacag aagaaacaga tcctaccttc gagtcctatg
    14701 atgaaaaata caatattcaa tcttatacaa atgttgcgat tcaaaaacac aaagcgaaaa
    14761 aagaagtagc tgtatttaga agaaatggaa aacatggagc tggtcttggt cctacaacat
    14821 tttgggcact caaacaagaa ttagaatttg ccggagtaga attttttcat ggactcaact
    14881 acaaagaagt cacaaaagac ggaatcacag taactttaaa aaatggagaa gaatttactt
    14941 atccctgcga ttctattgta ttatgtgtgg gacaagagaa agaaagttct ctctatgaag
    15001 agtttacttc ccgttatccg caaagaaaag tttttctcat tggtggagca aaagaagcaa
    15061 aagcaatcga tgcaaaaaaa gcttttctag acggactaga agccgcttat aatttatagg
    15121 aattaccatg aacttttttt cggaagaaga agatttaaaa ttaatatttg attctttaat
    15181 tgattggaag ccaatcgtag aagaagcaga gggagatttt ttagatcata aatactatct
    15241 aaaaaccaag gatcctagat ttgaaatggc tccttccgat ttagagagtg caattgattt
    15301 ctataaatcg tgtctaacgt cattaggaga atttttcgga gaagaagttt ttccctttgc
    15361 aaaagagatt gatcacaaag gtttaaaatt tgaaaatgga aaagtcattt ttccagaaga
    15421 attgatccaa gtctatgaaa aactaaaatc tactggacta ctaccctact ccattgcacg
    15481 aagcggtggg ggactcggtc ttcctgctac gataggcgca ctctttgcca tgattatggc
    15541 aagatcagat gtttcgattt gtatgacggt cactttatta aacttagctc agattataaa
    15601 tcgatacgga accaaagaac aaaatgaaaa gtatacagtg ccagcggcta gtggtgaatc
    15661 tctttttgca atggcactca cggaaccaga ttatggctca gatttaaaca atgtcagaac
    15721 agtagctact aaaatggaag atggtagcta tcgtatcaat ggaactaaga ggtttatttc
    15781 gcagggctgt ggacttggtt ctcaccccgc ttttctttta acactggcaa gaacaggaac
    15841 acaaaagggt gctagaggtc tttctgtttt cttagtgaag tcaagtgatg tgcaagtagc
    15901 aggaattgaa aagaaattag gaattcatgc atctcctaca tgtgagattg tatatgaaaa
    15961 ttcttatgca gaaattttag gagaggaagg tcttggactt acgcgttata cgacaggtat
    16021 gaccaacttt atgcgtcttg caagtgctgc cggtggtccg ggaggtggag ccgcggcact
    16081 ttatgaatgt aggaagtatg caaaagaaag aatgcaattt ggaaaaccaa tcgaggaaat
    16141 ccctgctgtt tctgaaatgc taaacaaaat agaaagagaa acaaatgcga tgaggctcat
    16201 cactctagaa acagcgagag tcattgattt ataccaacac acacaaatca gattagaaaa
    16261 aagtggtaag gaagatagag agatcagaaa aaacgagaga gttcaaagat acagtgcgtt
    16321 agcttctatc ttaaccccgg ttgcaaaata ctactgctca gaagaaggaa ataaacttgc
    16381 ttatcttgca atccaaatcc atggaggagt tggatacaca gaagatttca atgttgcaaa
    16441 actctatcgc gattctagaa tcaattccat ctacgaagga accagccaaa ttcacgtaag
    16501 aattgcgaca ggtgctattt ttgcaggcat gtcagaagaa ggactctttc gaaaatacct
    16561 aaaagaacta gtggctaata ttgatttacc ttcagattat ttaaaagaac atacagaact
    16621 tttagaaaaa gcaattcttt cctttaaaca aattccaatc gatacagaaa aagaaagagt
    16681 ggcagaaaat ctaatgatac tagtagcaag atattttgga agcattcttt acgagaattc
    16741 agttcagaaa ttaaaaggca aaccccaatt cgaaagatgg aaaaaagatg ccagaaacta
    16801 tgcaattgaa agcactgcga tcgctcattc ctgcttattt agaatccaaa atctttgact
    16861 cgaattacac gaaccaaagt ttatcaatta gtatttaaaa agaaaaatat tagctaatac
    16921 aataaatcaa taaaaatagg ggtttgggga tgatttagtc cccaagctgc tggaaggtaa
    16981 tgccgtcaac ttaagagaaa tgaaaaagaa ttaaccacga agagcacaga gaacacgaag
    17041 aaaagagaag gtttattatt tattcacttc gtgagtcttc gtgtattccc gggcatcgct
    17101 tgacgctcac catagccaag ctatgtggtt gaaaatcctt aaattgacag cattggccgg
    17161 caggttccct gccaaatatc tcaccgaata cgcgaagtgt gtaaggtgag atatttaact
    17221 tgtatttgat agcttaactc tttttattgg atttgtgttt gctttagaaa gttggattct
    17281 gtatttgctt acttttattt cctctacaat actactcaga ggattttatc attctaagtt
    17341 tttttctcat ttagatgttc caaaccatag gagcatgcat gactacccaa ctaaaaaatc
    17401 agctggccta gtttttatgg ggctatttat gattagcctc ttctatgtgc aaatctattc
    17461 aaaagacgga agtttttctt tactttccca aactcttcct ggtctaatgg tttgtctctt
    17521 aatcggtttt ttagatgata gatataattt tagttccaga agaaagctct tttttgaaat
    17581 tctttttttt attccttatg tttatctttt tttcccagac taccaagtct ttggatttac
    17641 ccttcctcta ccgcatattg tatttagtat tgttctcgca ggatatatta tttttgtaac
    17701 aaatctttgt aattttatgg atggattgga tctttacctt tctctttcta ttctttctag
    17761 tattggaatt tacttttcca ttcttcatcc aaatccaaat caagctataa cagttttatt
    17821 gctagtaaca tcgattcttt cttttcttta ttttaatttt ccaccagcaa gactttttat
    17881 gggggatact ggctctttgc cgctcggcta tatggttgcg atattacctt ttttaaatgg
    17941 aaatgagatt ccagatttaa gttatggttt tttactcatt cctgttttct ggatagatgg
    18001 ggtttttaca attctgattc gactgaagaa aaaagaaaat atctttcgtg ctcatagaga
    18061 acatctatat caaaagatta cattacaaaa attagataaa cggtttacta catttatctt
    18121 cagcctctgg aatttactta ctattccagt ttattttttt ctaaaagatt ctatcccgct
    18181 catggggata atctcgatta ttctttttct aaatggtttt ctctatacaa tcttaattcg
    18241 caaattataa tcctaatcat tacactagga atcgcatttt ctttgtttct aaagcaaaca
    18301 tctcattcat aaaaaacttc gaattaaaat taaaagtata gtttaagaat tgactctttg
    18361 tatttgagtc ctatatctat gattatgaaa attttagaaa gagtcctatc caagattttt
    18421 tctctagcat tactatggga aggaaccaaa aagaaagtaa cagtctcgga gtttctggaa
    18481 agagaaatca ttttagaaca tccctttcga gtcattagta tgtttctttt tacactctta
    18541 ctttttattt tgctaggttt aattggatct tattatacga tcaatcatat tctacaaatt
    18601 ttatcaaatc tcattcataa aaattttgac ttttcctcta ttgtgatttt actcatagca
    18661 gtcccactct cctcctttgg aattattgta tgcttttccg ctacatgtaa atatgcgcta
    18721 gaaatacaag ctctgatcct acttctctat cgatcgaaag aagtttatgg gaatgtagag
    18781 aaaattgaat actccattcg aactggaaag aattggaaaa cgatcacgaa acattgcact
    18841 gtttcttttc tctcacctct tggagaaaaa attacaatga ttggaacaaa ttcagatgga
    18901 tactttcatg atatcttaga acaagaaata gaagaaaata gacagattaa aattttactc
    18961 gatcctaaga atcctagaat cgctaggatg atccataaag aatcatagaa atagatttcc
    19021 ttctcttatg actctgtatc aaagacaagg actgagcata gtccaatcaa gcgtttaaaa
    19081 aaggttttga ttcctatcat tcaatattga aaatagattt atgcagaaca aaagtgtaag
    19141 aagagacatt tccatccttt tcttgctctc cttcaaccga gctagattaa atgctacttt
    19201 attcattcat tagaattatc gtaacctatc aggctgctaa gttactttta tcaagagaat
    19261 ataattttaa tat
//
LOCUS       DAOQOS010000107        18724 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_1487_length_18724_cov_5.802185, whole genome shotgun sequence.
ACCESSION   DAOQOS010000107 DAOQOS010000000
VERSION     DAOQOS010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 18724)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18724)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18724
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1487_length_18724_cov_5.802185"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            354..3944
                     /gene="nifJ"
                     /locus_tag="PLF66_19355"
     CDS             354..3944
                     /gene="nifJ"
                     /locus_tag="PLF66_19355"
                     /inference="COORDINATES: protein motif:HMM:TIGR02176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HMZ66257.1"
                     /translation="MENKYVVLDANEAVADIAYRLSEVIAIYPITPSSPMGEWADAWM
                     SQSRKNLWNTVPSVIEMQSEGGAAGALHGALQTGSLATTFTASQGLMLMIPNMYKIAG
                     ELTACVIHVAARSLAAQGLSIFGDHSDVMAVRQTGFAMLCSDSVQMAHDFALISHIAT
                     LESRIPFLHFFDGFRTSHEIAKIEMIEDSIIRKLIKEDSVHAHRKRALNPNQPVLRGT
                     AQNPDVYFQARETVNQYYPQCGEILERVLKEFGEMTGRKYEAFEYFGDENPDRVIVIM
                     GSGTEAVHETVEFLNSKGEKVGVIKVRLYRPFQVEKLIAKIPSSVKSIAVLDRTKEPG
                     SAGEPLYLDVVNALFQDQISNNKKFKNLPKVIGGRYGLSSKEFTPAMVKSIYEELKKE
                     NPKNSFTVGILDDVTNLSLSYDSGFSLEKKDTIRSVFYGLGSDGTVGANKNSIKIIGE
                     NTSNYVQGYFVYDSKKSGSMTISHLRFGKSPIRSTYLIDHPNFVACHQPIFLDRFDML
                     SGIEEEGTFLLNTSQPKETVFASLPGKIQKDILEKKLNFYIIDAYKVASHAGMKNQIN
                     TIMQVCFFHIANIIPKEEAIAAIKESIEDTYGKKGGEIVLRNMRAVEDALAHLYKIDY
                     SEFKKPTKEMIPSGVSISDKAPEFVKTVLGKMLSAKGDLLPVSAMPIDGTYPTGTSQW
                     EKRRIAQEVPVWDKNVCIQCGKCSYVCPHGVIRVNAFTEDKLKNSPSSFEVGDSRVPE
                     LKEKGFKFTLQVSVDDCTGCGICVDICPAKNKSEVRLKAINMTPIEKIPDTHKEDWDF
                     FKTIPPIDRSLLNIHQIRQQQMQEPLFEFSGACAGCGETPYVKLVSQLFGDRMVVANA
                     TGCSSIYGGNLPTTPWAKNNEGRGPAWSNSLFEDNAEFGYGFRVSIDKQRDAAIELVG
                     ELKEKIGVSLSEEILNAKQSEESEIYEQRERVAKLNQILKGFAKEDEKARHLLELSDS
                     LVKKSVWIIGGDGWGYDIGYGGLDHVLASGRNINILLLDTEVYSNTGGQMSKSTPKGA
                     VAKFATGGKPVRKKDLGLIAMSYGNVYVATVAMGAKDEQTLRALMEAEKYDGPSIVIA
                     YSHCIAHGINMTEGMKHQKAAVDSGQWILYRYNPDRAENGLNPLEIDSAAPHAPVRSY
                     FESENRFKMLTKSKPDIAKNLFEQAQKDINNRYKHFEFLAEKKKEEDKHD"
     gene            3937..4941
                     /locus_tag="PLF66_19360"
     CDS             3937..4941
                     /locus_tag="PLF66_19360"
                     /inference="COORDINATES: protein motif:HMM:NF005741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase-like protein"
                     /protein_id="HMZ66258.1"
                     /translation="MINLSTKYMGLSLRTPLVASAGPTSYHLDDIKKMEDAGISAVVL
                     YSLFEEQIEKEHQELHHHLNYGTHSFAEAITYFPQSGDFEVGPDEYLEYIRKVKESVS
                     VPIIASLNGYTSGGWTYFAKLIEEAGADGLELNLYNVPTDANVTGKELEEDFAETLWQ
                     VRKTVSIPLALKLSPYFSNLMNCAKKFANMGANALVLFNRFYQPDFDLEHLEVKSHVH
                     LSTPHDLRLSLRWIALLYSRIHSDLAGTGGVHRAEDVLKLLMAGANVTMLCSVLIREG
                     IKKISVIEKDLVSWMEKHEYESVSQMIGSMSAAKYHTPFEYERAQYIKALHSVHVHHD
                     "
     gene            complement(5310..6704)
                     /locus_tag="PLF66_19365"
     CDS             complement(5310..6704)
                     /locus_tag="PLF66_19365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008591675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66259.1"
                     /translation="MNTKEYLDLYLQENILESETLHNMRNVISEFVARAPKILVTKCI
                     DGRVHGSKHKGYPATTIRFGRTDGNIVSTDKNNYWFWNRIDNIVNNALCNTPDMPALF
                     IAYMHKSDLGLGCAAHNNNEETAFQAIESQTNAVKTIFKREELFVLEGITNTDTMAET
                     LIFENSIQLSSEEIIKNFDLKNLTDVFHKGFLKYPIKDPATSRYVNFKTPEELMQGDL
                     PQFHFDFQTSLAMKSFLLLEISKIIYEKDFSSQKLIQPDVLNAILSILNKIKSLPNSL
                     FAPFLYQCFWNIGYSLYQRKKLNNMEKLEKEKHLDHDEELICYGDGFELLPRNKAILV
                     KTGRGDDENALLVAKKVLEKNRMRKHSEIDPMIHLNIEISGELMTWDDFNENISSRMQ
                     TMIRHIDEVFGQNIVILTTYSYHDQKRFYPIITKKDNRLIYPVNLLSEINNSIRFSNM
                     ALKSRESLFAMERK"
     gene            complement(6933..8894)
                     /locus_tag="PLF66_19370"
     CDS             complement(6933..8894)
                     /locus_tag="PLF66_19370"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HMZ66260.1"
                     /translation="MYSLKMISKCVFHFKYLFLILYVFSLSAEEGIPKSDSILIHSTL
                     HYLEDKSRTLNLEEIENTVYQGEFKPIGEKLKSFGYTHSIYWYYTDYPLPINLENFLL
                     EIPNPILDSVEIFYRTKTGEIQTKKVGRLISFRSREYENRNFLISLDPIPVGRIYFKV
                     DTESSLQLPVLLWEKKTFFEKDQYSFLFQILFIGIVGAMFLYNSLLGISLKDKLYLYY
                     ILYLSSVSLFLTGINGLNAQLLFRDSPNLDKYSLPVLLSLNNLGNVLFAKSFLNLKNQ
                     NTTYEKIFRFLIGISFLFFLFSFLLSYISISRLIVFTAVINVSFSIYVAVRIALKGYR
                     PAKIYLLAWGVFLFGAVIYAFSRFGFISVNAFTNHISQIGVAIEAIFISFALADRIKI
                     IELEKEKAQAEMLVAVRQTERLKSELELAKRIQQSIIPKRTPNLKGLEIASWYRPMEI
                     LGGDFYDFRTVDGNLSCIIADVSGHGLPAALVVSTVKTAFWFQEEHINSPTSLLHNMN
                     RILIDKSGGEFITACYLFIDIQNKILKTGNAGHNPLILYRKSTEELLTIRPSGGALAI
                     FDNPKFLSEDISIQTGDRIIIYTDGLFEAFNEKEEAFGEDRIFRLLKENITKSPKDFG
                     LSLMNEVTLWSGGEHNLQDDIALIVIDVL"
     gene            complement(9093..10064)
                     /locus_tag="PLF66_19375"
     CDS             complement(9093..10064)
                     /locus_tag="PLF66_19375"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HMZ66261.1"
                     /translation="MSQEEKPLAIGDTAPDLLNLKWVKGNPTNIGGKLTLVECWATWC
                     GPCLRTIPHLTELQKKYSNELQIIGISQEDEETVSPFVENMGDQMSYRVAVAPDEVYN
                     AYMEGIPGIPHSFLINTKKELIWHSHPMEIDAILDGYIKGKITEENLKQIAVKKEEYN
                     ASLEILQSGQFENPNPIVEKMIDVGLSLLYLTPDNSDILRTLFYMTTAIGRPDLFESI
                     CKGVDKTKFSTQNLASYLDNALLEYVGSSSIALNYSIDWLNFLSSKEENPYILSLCAR
                     FLYTIGLLDRAIDTLKKASTLSGDDSSFEGQLSFYNLYKEAQGKIKF"
     gene            complement(10354..10773)
                     /locus_tag="PLF66_19380"
     CDS             complement(10354..10773)
                     /locus_tag="PLF66_19380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66262.1"
                     /translation="MKKLTYILIVLAFISCGKKAEVTWDSALYSDPELTQKITDVKKG
                     TVGIATGYRNHKWVAKQSIHMNIDGKEGYISPKHVVIGQNPAKSVFVWGYSEKYKKFF
                     DPKDKKHYKDGYEFPDLAKLPKEKLPLDELVKDSVVE"
     gene            complement(10784..12013)
                     /locus_tag="PLF66_19385"
     CDS             complement(10784..12013)
                     /locus_tag="PLF66_19385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002148435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66263.1"
                     /translation="MKAEIQDNLINFKLSGATRNTLFLMILIGIVSFVGGFFGLHFES
                     RHAGGHSNPAWSALLVATFFILGISITGIFFTAIGHITGAHWLVTMRRITESYSKFLP
                     IGVVLLAILVLFGTHDLYEWSHTDNAAVMSDKLIQHKSGWLNTPFFIGRLIFIGAIWA
                     LFGFLFYKHSVAQDSDKDVSHTSANAKLAGGFLLFFAISFSVVSFDLLMSLTPHWFST
                     MWGVYCFAGAYQTGLSSLAVMIFYLKKNGYFGDALNENHIHDVGKFMLSFCVFWAYVG
                     FSQFMLQWYANLPEETFWYEQRLMGGWTGITIAIPFIKFIVPFGLLLNRPNKRDINFL
                     YKVAVWILVSHMIELYWIVFPSNFEHFNIAGLGVTAGVTIGMVGLFGFIVLKGMESAK
                     LVPVGDPRLEQSLGHHQ"
     gene            complement(12021..12608)
                     /locus_tag="PLF66_19390"
     CDS             complement(12021..12608)
                     /locus_tag="PLF66_19390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000878639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HMZ66264.1"
                     /translation="MNLKKFIIFIFLAVAISNCEYSKPVHEFFPDMYDSPARESQELD
                     NFHNSYGRRLPPEGTIPVGYYPYEFADIATPDEMTKDQGLKNPYPTKTTLANYQRGES
                     RYQIFCSPCHGVAGSGNGNVVGPYPRFSVRPPSLVGAKIQGWTDGQIYHIITMGRGLM
                     ASYAAQIEPDDRWKIILYIRKLQEAEQKAKAPANK"
     gene            complement(12611..13171)
                     /locus_tag="PLF66_19395"
     CDS             complement(12611..13171)
                     /locus_tag="PLF66_19395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3341 domain-containing protein"
                     /protein_id="HMZ66265.1"
                     /translation="MYLPKLEQFHSYTETSEGVFGLFDEPEQILEAARQTRDKKYYVG
                     FDCLTPFPVHGLDDAMGLPRSGIPWITLAMGLVGGTFGFGYQYLTHAVDWPINFSGKA
                     YNAWPAFVPVTFEMTIFMAGISSALALFFLSRIPVVGRKILHRDITSHKFALWIPATA
                     PGYKEADTVNFIKSLGATDVTVVKKG"
     gene            complement(13173..14537)
                     /gene="nrfD"
                     /locus_tag="PLF66_19400"
     CDS             complement(13173..14537)
                     /gene="nrfD"
                     /locus_tag="PLF66_19400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004458091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysulfide reductase NrfD"
                     /protein_id="HMZ66266.1"
                     /translation="MTVAIKEKLGIVPLVEGNKSYKQVTEDILKPTESFPTKVWWTAF
                     GLALTVTLVDLGIIGYLMYEGLYILGINNPVGWGFFIVNFVFWIGIGHAGTLISAVLH
                     LFRQEWRTGINRAAEAMTIFAVMVAASSLIVHVGRPWLGFWLFPYPNERGPLWVNFRS
                     PLIWDTFAVSTYFSISLVFWYIGLIPDIASVRDRTTNKFKYLIYDAMSFGWVGSNKAW
                     SHLEMVSMILAALSTPLVLSVHTIVSFDFAASLLPGWHTTILPPYFVAGAIFSGFAMV
                     VTLMVIAREMFNLKEYITMKHMENMNKVIMVTGLMVGYAYATEFFIAWYSGNEYEGFT
                     FVNRVLGPYWWAYWIMVSCNVLSPQVFWFKSMRTSIPVMFVVSIFVNIGMWFERFVIV
                     MTLHRDFLPSSWALYTPTIFDYAMLIGTFGIFFTLFLLFCRILPVIAAAEVKSIMPTK
                     GGHH"
     gene            complement(14562..17633)
                     /locus_tag="PLF66_19405"
     CDS             complement(14562..17633)
                     /locus_tag="PLF66_19405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000372298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HMZ66267.1"
                     /translation="MSNNSFQEERKSHWQSYETKDKSDLKQIQNQEFYTSPDPIISRI
                     KTGDYDRRTFLKLMGASSAMLTLNCIQKPQEKIVPYVKAPDYVKPGQSTYYASTCGGC
                     SAGCGILVKTRDARPLKLEGNPANPISKGALCAVGQSSILDLYDPDRAQEPAEVKNGA
                     AVTIKWADLDTQVKTQLAALKGKTRIVTGPIDSPSTKAVIAEFLTAVGGGKHYEIDVT
                     SVEESIAIAANESYGKPVVPNYHFDKAKVILSIDADFLGTWISQVEYSKQFASRRKIK
                     DVNSKINKFYAVESVPSVTGSNADKRIAIKSGDARRFAMAIAVALGATTGYTLESLSS
                     EIGVDAKLIQQIAKDLLAAKGESLVVAGGTSSQTTDAVDLQIAVNLINSFLDNDGKTI
                     DPVNNRSESAHLYSANLASLQKELNAGEVGVLFVYGTNPVYQLPSLNLKDAFAKAGLY
                     VSLADRLDETASFAKYLAPTTHYLESWGDREAVKGVVSITQPAIRPIYNTRSLEDSLI
                     VWAGGKLGASPSYYEYLKSAYTKKLGSKFNWEDFLRNGSLFKEADIKSNKAGRSFKAS
                     AVAKLPAKESGKSLTLSLYTTVAIGDGTHANNPTLQELPDPITKATWENFLGISPGQA
                     DALGIKSNDVVKVKAGSREITLPALIVPGLHNETVSIALGYGREKAGKVGNVGKNSYN
                     LANIVDGKVQYAGITVTLEKTGKVSKVATTQDHHMMNPTATPGKGPKGGLYKPYEHDR
                     PLIQTTSFEDYVKNPESGKAESEIPKLNEKEMSRGLNPQFEYKGYRWGLSIDLSLCTG
                     CSACVTACQVENNIPVVGKSEVLVGREMHWIRIDRYYIGDPLKPESLEIAHQPVMCQH
                     CENAPCETVCPVAATTHSSEGLNDMVYNRCVGTRYCSNNCPYKVRRFNWMQHWKDSDY
                     QREPRHLGLNPEVTVRSRGVMEKCTFCSSRIAEKKIKAKNEGRTLKDGELKTACQETC
                     PADAITFGNTNDPEAQVTKNAHDHRAYRILEFLNVLPQVSYLTRVRNKVNV"
     gene            complement(17655..18194)
                     /locus_tag="PLF66_19410"
     CDS             complement(17655..18194)
                     /locus_tag="PLF66_19410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004508958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HMZ66268.1"
                     /translation="MNIQAIKITMPLITVAAVAYLIFSPSRYVGYAPDQPLPFNHKIH
                     AGDNKIDCRYCHTSVETSAHASVPPTATCMNCHSNVANKTNDVKWLTKQFEGEKSTGN
                     SIEGKGKPIQWIKVHDQPDFVYFNHSRHISRGVDCSKCHGNVAEMVKVKQVESLNMGY
                     CVNCHRENNAPNDCSTCHR"
BASE COUNT         5893 a         3381 c         3504 g         5946 t
ORIGIN      
        1 gtattctatt tgtattgcat attataatga attgctaaga taatgtaatt tggaacgatt
       61 ccatattctg actctagaaa gcagtctttg gttgttctac aaacaagaat ctttgcgtat
      121 catcatagga atgagttttt agacaggttt taaataattg atagaaacca tttcttttgg
      181 aaaatctgct cttccaaaaa atcataaatt tgtaagaaaa cgctctataa tgatatttaa
      241 gaagctattc gcatttatgc taaataattt taaatctaga atagaggaaa cctatgataa
      301 aaatcaggtt cgttaaaact tgcaattatg aatgaaaatg ttataataaa aggatggaaa
      361 ataagtatgt agttttagat gcaaatgaag cggttgctga tattgcctat cgtttaagtg
      421 aagtaattgc catttatcca atcactccat cttctccaat gggggaatgg gcggatgctt
      481 ggatgagtca gagcagaaaa aacctctgga atacggttcc ctctgtaatt gaaatgcaaa
      541 gtgaaggagg ggcggcagga gctttacacg gagcacttca gacgggatct ctcgcaacaa
      601 ccttcactgc ctctcaggga cttatgctta tgattccaaa tatgtataag attgcaggtg
      661 aactgactgc atgcgttata catgtagctg ctaggtctct tgctgcgcag ggtctttcca
      721 tatttggaga tcattccgat gtgatggctg tgcgacaaac tggctttgcc atgttatgct
      781 cagattctgt tcagatggct catgactttg cacttatttc gcatattgca actttagaaa
      841 gtagaatccc attcttacat ttctttgatg gatttagaac atctcatgag attgcaaaaa
      901 tcgagatgat tgaggatagt ataattcgaa aattgatcaa agaagattcc gttcacgcgc
      961 atagaaagcg cgctctcaat ccgaatcaac ctgtgcttcg aggaacagct caaaacccgg
     1021 atgtatattt tcaagctaga gaaacggtta accaatatta tccgcaatgt ggagagatcc
     1081 tagaaagagt ccttaaagaa tttggagaaa tgacaggtag gaagtatgaa gcgtttgaat
     1141 actttggaga tgagaatccg gatagagtaa ttgtgatcat gggatcgggg actgaggcag
     1201 tgcatgaaac tgtggaattt ttaaattcaa aaggggaaaa agtaggggta atcaaagtta
     1261 gactttatcg tccttttcag gtggaaaagc ttattgctaa aattccatcc tcagtcaaat
     1321 ccattgctgt tttagataga accaaagagc caggtagtgc aggagaacct ttatatttag
     1381 acgtagtcaa tgctcttttt caggatcaaa ttagtaataa taaaaaattt aaaaatctac
     1441 cgaaagtcat tggaggtaga tatggattat cctctaaaga atttacacct gcaatggtaa
     1501 agtccattta tgaagagctt aaaaaagaga atcctaaaaa tagttttacc gttggtattc
     1561 tagatgatgt tacgaactta agtctttctt atgacagtgg atttagctta gagaagaaag
     1621 acacaattcg ttctgttttc tatggactcg gttcggatgg aacggtaggc gcaaataaaa
     1681 actcaataaa aatcatcggt gagaatactt cgaattatgt gcaagggtat ttcgtttacg
     1741 attccaaaaa atctggttct atgacaattt cacacttgcg gtttggcaag agtccaatcc
     1801 gttccactta tctaattgat catcctaatt ttgtagcgtg tcatcagcca atctttttgg
     1861 atcgatttga tatgctttct gggatcgagg aggagggaac atttttatta aatacaagcc
     1921 aacctaaaga aacagttttt gcatctcttc caggaaaaat tcaaaaagat attttagaga
     1981 aaaaattaaa tttctacatt atcgatgcct ataaagtagc aagtcatgcg ggaatgaaaa
     2041 atcaaatcaa cacgattatg caggtttgct tttttcacat tgcaaatatc atcccaaaag
     2101 aagaagcaat tgcagcaatc aaagaatcta tcgaagacac atacggaaag aaagggggag
     2161 aaatcgttct aagaaatatg agagcagtgg aagatgctct tgctcatttg tataagatag
     2221 attatagtga atttaagaaa ccgactaaag aaatgattcc gtctggcgtt tctatttctg
     2281 ataaagctcc tgagtttgta aaaacagtgc ttggtaaaat gctttcagca aaaggagatc
     2341 ttttaccagt cagtgcaatg ccgatagacg gaacctatcc aacaggaacc tctcaatggg
     2401 aaaaaagaag gatcgcccaa gaagttcctg tttgggataa gaatgtttgt attcagtgcg
     2461 gcaaatgttc ctatgtgtgt ccacacggag tgattcgagt gaatgcattt acagaggata
     2521 agctaaaaaa ttcaccttca tcttttgaag ttggggactc aagagttcct gaattaaagg
     2581 aaaaaggttt taaatttact ttgcaagtct ctgttgatga ttgcacaggt tgtggaattt
     2641 gtgtagatat ttgtcctgct aaaaataaat cggaggtaag attgaaagca atcaatatga
     2701 ctccgataga aaaaattcct gatactcata aagaagattg ggattttttc aaaacaattc
     2761 cgcctatcga tcgaagttta ctcaatattc atcaaatcag acaacaacaa atgcaagagc
     2821 cactctttga attttcggga gcctgtgcag gttgtggaga aactccttat gtgaaattgg
     2881 tttctcaatt atttggagat agaatggtcg tcgcaaatgc aactggttgc tcctctattt
     2941 atggaggaaa tcttcctaca actccttggg caaaaaataa tgaaggaaga ggtcctgctt
     3001 ggtcaaattc tttatttgaa gataatgccg aatttggata tggatttaga gtatcaatcg
     3061 ataaacaaag agatgcagcg atcgaacttg taggtgaact caaagagaaa atcggtgtta
     3121 gcctctcaga agaaatacta aatgcaaagc aatcagagga atctgaaatt tatgaacaaa
     3181 gagaaagagt tgcaaaatta aatcaaattt taaaaggctt tgcaaaggaa gatgaaaaag
     3241 caaggcatct attagaactt tcagattctc ttgtgaagaa aagtgtctgg atcattggag
     3301 gagatggttg gggatacgat attggatatg gtggactcga tcatgtgctt gcgagtggta
     3361 gaaatataaa tatacttcta ctcgatacag aagtttattc gaatacaggt gggcaaatgt
     3421 ctaaatctac gcctaagggt gcagttgcta aatttgccac aggtggaaag cctgttcgga
     3481 aaaaagactt aggtcttatt gcaatgagtt atggcaatgt atatgtggca acagttgcga
     3541 tgggagcaaa ggatgaacag acgttacgcg ctctaatgga agcagagaag tatgacggac
     3601 cttcgattgt aattgcctat agccattgta tagctcatgg aattaatatg actgagggca
     3661 tgaagcatca aaaggcagct gtagattctg gacagtggat tctttatcga tacaatccag
     3721 atcgagctga gaatggatta aacccactcg aaatagattc agctgcccca catgcacctg
     3781 ttcgaagtta ttttgaaagt gaaaacagat ttaaaatgct cactaaaagt aaaccagata
     3841 tcgctaagaa tctatttgag caagcacaga aagatatcaa taatcgatat aagcactttg
     3901 aatttttagc tgagaagaaa aaagaagagg ataaacatga ttaatctctc cacaaagtat
     3961 atggggctct cgcttcgaac tccactcgtt gcatctgcag gaccaacttc ttatcattta
     4021 gatgatataa aaaaaatgga agatgctgga atctctgctg tcgtcttata ttccttattt
     4081 gaagaacaaa tagaaaaaga gcaccaagag ctacaccatc atttaaatta tggaactcat
     4141 agttttgcag aggcaattac atattttcct caatcagggg attttgaagt tggaccggat
     4201 gaatatctag aatacattcg aaaagtaaaa gaatcagttt ctgtaccaat cattgcgagc
     4261 ttaaacggtt atacttctgg tggttggacc tattttgcaa aattaattga agaagcggga
     4321 gcagatggat tagaactaaa cttatataat gttccgactg acgcaaatgt aaccggtaaa
     4381 gaattggaag aagattttgc agaaacttta tggcaggtta gaaagacagt ttctattcca
     4441 cttgcattga aactcagtcc ttattttagc aatttaatga actgtgcaaa gaaatttgca
     4501 aacatgggag ctaacgcact cgttctattc aatcgttttt atcagccaga ctttgattta
     4561 gaacatctag aagttaagtc gcatgtacac ctatccaccc cgcatgattt aagactctct
     4621 cttcgttgga ttgcactttt gtattctaga attcattctg atcttgcagg aacaggtgga
     4681 gtgcatagag cggaggatgt tttaaaacta cttatggcag gagctaacgt cacaatgctt
     4741 tgctccgttc tgataagaga agggattaag aaaatctctg tgatcgaaaa agatttagtt
     4801 tcttggatgg aaaagcatga atacgaatct gtttcgcaaa tgattggaag tatgagtgct
     4861 gctaaatacc atactccttt tgaatatgag agagcccaat acataaaagc attacacagt
     4921 gtgcatgtgc accacgatta aaactatcgc tagtttgata taataaaata tttaaaagat
     4981 tatcagcgat acgcaccgag gagaccgata aaagatacga taattcaaaa tacatcgttt
     5041 cttcatcgct cagttatcgg tgtgcacacg ttcgataggc tcagtgcaat gctattttaa
     5101 gaaaaactct taaagattac cttattatgt tgcgaccctt aggaagcaat attgagtttg
     5161 aacacggatg gacaccgata aaatattagg aatcattaaa accatccgtg tctatctgtg
     5221 ttcacccgtg gtgaaaaatt cttaacttga tagcattggg ctcattgcag gtcggtggtg
     5281 aaaaaaatta catcaaatcc atgttaagac tactttcttt ccattgcaaa aagagactcc
     5341 ctgcttttta acgccatatt cgaaaagcgt attgagttat tgatttctga aagtaggttt
     5401 actggataaa tcaaacgatt gtcttttttt gtaatgatag gatagaatct tttttgatca
     5461 tggtatgaat aagttgtgag gataacaata ttttgcccaa agacttcatc tatatggcga
     5521 atcatagttt gcattcttga agaaatattt tcattaaaat cgtcccaagt cattagttcg
     5581 ccgctgattt ctatgttcaa atgaatcatt gggtctatct cactatgttt tctcattcta
     5641 tttttttcta atactttttt agcaacgagt aaagcatttt catcatctcc acgaccggtc
     5701 tttacaagaa tcgctttatt tcttgggagg agttcaaatc catccccata acaaattaac
     5761 tcttcatcat gatctaaatg tttttctttt tcgagcttct ccatattgtt tagtttcttt
     5821 ctttgataga gagagtagcc tatattccaa aagcattggt agagaaaagg agcaaagagt
     5881 gaatttggaa gagatttaat tttatttaaa atcgatagga ttgcatttaa tacatctggc
     5941 tgaattaatt tctgagagga aaaatctttt tcatagatta ttttagaaat ttctaaaagt
     6001 aaaaaagact tcatagcaag agaggtttga aaatcaaaat gaaactgagg caaatcccct
     6061 tgcatcaatt cttcaggagt tttaaagttc acataacgag aagttgccgg atctttgatt
     6121 ggatatttta aaaatccctt atggaaaaca tctgttaaat tttttagatc aaaattttta
     6181 atgatttctt cggaactcaa ttggattgaa ttttcaaaaa tcaaagtctc agccatcgta
     6241 tccgtatttg taattccttc caatacgaat agttcttctc ttttgaaaat agtttttact
     6301 gcatttgttt gactttcaat ggcttgaaaa gctgtctctt cgttattatt gtgagcggca
     6361 caaccaagac cgagatctga tttatgcata taagcgataa atagagcagg catgtcaggt
     6421 gtgttacaaa gagcgttatt tactatatta tcaattcgat tccaaaacca gtaattattt
     6481 ttgtctgtag aaacaatatt tccatctgtc ctaccaaaac ggatcgttgt tgcaggatag
     6541 cctttgtgtt tacttccatg cactctacca tcgatgcatt ttgtaactag gatcttagga
     6601 gctctagcta caaattcaga aatcacattt cgcatattgt gcaatgtttc tgattctaat
     6661 atgttttcct gtaagtaaag atctaaatat tcttttgtat tcattgacgt cgtaataact
     6721 atcatgaagt gagcgtaagg atttagcaag tcatttagac tatcatttca tttatgtttt
     6781 gtttttaatt agaaacatag attttcgttt ggaaacagaa aactttattt cagttattga
     6841 ttttaaagaa tccttcctgt tgtcattttt ctaatcgatt tcaaaatcat tcatttagaa
     6901 tcagttgtta taggttttgg gttggatgtt tttcacaaaa catctataac aattaacgca
     6961 atgtcatcct gcaaattgtg ctctccacca gaccagagag tgacttcatt cataagactc
     7021 agtccaaaat cctttggtga ttttgtaatg ttttctttta aaagacgaaa gattctatct
     7081 tctccaaaag cctcctcttt ttcattaaag gcttcaaaaa gtccatccgt ataaatgata
     7141 attctatctc ctgtttgaat agagatgtcc tcggataaaa atttaggatt atcaaaaatg
     7201 gcaagtgctc ctccgctggg gcgaatggtg agtaactcct ccgtcgactt tcgatagaga
     7261 atgagtggat tatgacctgc atttcctgtt tttaaaattt tattttgaat gtcaataaag
     7321 agatagcaag cggttataaa ttcaccgcca cttttatcga ttaaaattcg attcatattg
     7381 tggagtaggc tggttggaga attgatatgc tcttcttgaa accagaaagc tgttttcact
     7441 gtcgatacaa ctaacgcagc gggaagccca tgaccggata catctgctat gatacaactt
     7501 agatttccat ctacagttct aaaatcatag aagtctcctc ctaaaatttc cattggtcga
     7561 taccatgaag caatctcaag tccctttaaa ttcggagttc ttttggggat gatggattgt
     7621 tgaattcgct ttgccagttc tagttctgac ttcaatcgtt ccgtctgacg cactgctact
     7681 aacatttccg cctgtgcttt ttctttttct agttctatga ttttaatgcg gtctgctaaa
     7741 gcgaaagaaa taaaaatagc ttcaatcgca actccaattt gagagatatg gttggtaaat
     7801 gcatttactg aaataaaacc gaatcgagag aaggcgtaaa tcactgctcc aaatagaaaa
     7861 accccccagg caagtagata aattttggca ggtctatagc ctttcagtgc aattcttacc
     7921 gcaacataaa tcgaaaaact gacattaatc acagcggtaa atacaatcaa tcgactaatg
     7981 gaaatgtagg atagaagaaa ggaaaaaaga aagaacagaa aagaaattcc aattagaaat
     8041 ctaaaaattt tctcataagt tgtgttttga ttttttaaat ttagaaaaga ttttgcaaat
     8101 aaaacatttc ctagattgtt taaacttagt agcacaggaa gagagtattt gtctaagttt
     8161 ggtgagtcgc gaaatagtaa ttgcgcattt agaccattga tgcctgttaa aaatagacta
     8221 acagagctaa gatataaaat ataatacaaa tacaatttat cttttagaga aattcctaaa
     8281 agactattgt ataaaaacat agctcctaca attccgataa atagaatttg gaaaagaaat
     8341 gaatattgat ccttctcaaa aaaagttttt ttctcccaaa gaagaacggg aagttgcagt
     8401 gaactttcag tatcaacttt gaaatagatt cttccaacag gaattggatc taggctgatt
     8461 aaaaaattcc tgttttcata ttctcggctt ctaaaagaaa taagtcttcc tacttttttt
     8521 gtttggattt ctcctgtctt agttctgtag aagatttcga ctgagtctag aataggattt
     8581 ggaatttcta aaagaaaatt ttctaaattt atcggaagcg gataatcagt gtaataccaa
     8641 tagattgaat gtgtgtaacc aaacgatttt agcttttccc caattggttt aaattctcct
     8701 tgatatactg tgttttctat ttcttctaaa ttcaaagttc tagatttatc ttctagataa
     8761 tgaagggtag agtgaattag aatactatca ctctttggta ttccttcttc cgcactcaaa
     8821 gaaaaaacat agagaattag aaataaatat ttaaaatgaa aaacacattt agatatcatc
     8881 tttaaactat acataaactt ttttcaagaa acctatgata cgatcttctt gtgtgtcaat
     8941 gcgaatcatc caatctattt aaggattttt caccacattg agttctggca cagaagaggg
     9001 catgtcgaac aggaatgaaa actcttaact tcgtgctctt cgtgatgaaa acttcttaat
     9061 tttaatccat cgaacgatgc atcaatctgt atttaaaact ttattttacc ttgtgcttct
     9121 ttatagagat tataaaaaga aagctgtcct tcaaaactgg aatcatctcc actgagagta
     9181 gatgctttct tcaatgtatc aattgctcta tctagaagac ctatcgtata taaaaaccga
     9241 gcacaaaggg agagtatgta tggattttcc tctttgctag aaagaaaatt cagccaatca
     9301 atcgaataat ttaaagcaat cgaggagctt cccacatatt ctagtaatgc gttatctaaa
     9361 tagcttgcta gattttgggt tgagaacttt gttttatcca ctcctttgca aatggattca
     9421 aataaatcgg gtcttccgat tgcagttgtc atataaaaca aagttcttaa aatatcagaa
     9481 ttgtcaggag tgagatagag taaacttaat ccaacatcta tcattttttc tacaattgga
     9541 ttcgggtttt caaactgacc ggattgtaga atttcgagag aagcattgta ttcttctttc
     9601 ttgactgcga tttgttttag attttcttct gtgatttttc ctttaatgta gccatctaga
     9661 atcgcatcaa tttccatagg gtgtgaatgc caaatgagtt ctttctttgt attgatcaaa
     9721 aaggagtggg gtatcccagg aataccttcc atataagcgt tatagacttc gtctggggca
     9781 actgctactc ggtaagacat ctggtctccc atattttcta cgaatgggga aacagtttct
     9841 tcatcctctt ggctaatgcc gattatctgc aactcgtttg aatatttttt ttgtagttct
     9901 gttaaatgag gaatggttct tagacatggt ccgcaccaag tagcccagca ttctactaac
     9961 gttaatttcc cacctatatt cgttggattt cctttgaccc attttaaatt cagtaggtct
    10021 ggggctgtat ctccaattgc taatggcttt tcttcttgtg acatacattc ctccgatttt
    10081 ttagaaatgt cgtttctaca atgctatttt gtagagagaa ggcaatcaaa tattttaggt
    10141 gtgaagtttt agaggaatta gctctttaat aaagggaagc ttaaaagaat accaccgatg
    10201 ggcaccgata agaaaacgat ttaaatttat cgtatcttta atcagtgcga tgtggtgagc
    10261 agagtcgaac cattaatcgt ctttatcggt ggtaatcttt tgagagcctc ctgatcaata
    10321 acattgagtt atttaggagg cttcttttat ttgttactct acgacgctgt cttttactag
    10381 ttcgtcgagt ggtaattttt cttttggaag ttttgctaaa tctggaaatt catatccatc
    10441 tttgtagtgt tttttgtctt taggatcgaa aaacttttta tatttttctg aatagcccca
    10501 aacaaaaact gactttgctg gattttgtcc aatcacaaca tgcttaggag aaatatatcc
    10561 ttcttttcca tcgatattca tgtgaatgga ttgtttcgca acccatttgt gattgcgata
    10621 gccggttgca attccaacag ttcctttttt aacatctgtt attttttgtg ttaactctgg
    10681 atcagagtag agagcagagt cccaagtgac ctctgctttt ttcccacagg agataaatgc
    10741 taaaacaatg agtatataag ttaatttttt catcttcttc tccttattga tgatgtccta
    10801 aagattgttc taatctagga tcgccgacag gaactaactt agctgactcc attcctttta
    10861 gaactataaa accgaacaat ccaaccatac cgattgtgac tcccgctgtt acaccgagtc
    10921 ctgcaatatt gaaatgctca aaattacttg ggaaaacgat ccaatagagt tcaatcatat
    10981 gggatactaa gatccaaact gctactttat ataagaaatt gatatctctt ttatttggtc
    11041 tatttagaag aagaccaaat ggaacgatga atttgatgaa tggaattgca attgtaattc
    11101 ctgtccatcc acccattaat ctttgctcat accaaaaagt ttcttccggt aagtttgcat
    11161 accactgtaa cataaactgg ctgaaaccta cgtatgccca gaatacacaa aaggataaca
    11221 tgaatttacc cacatcatga atgtggtttt cgtttaacgc atctccaaag tatccattct
    11281 tttttaagta aaagatcatg actgcaagag atgagagacc agtttggtag gctcccgcaa
    11341 agcaataaac tccccacata gtagaaaacc aatgtggtgt aagagacatg agtaagtcaa
    11401 aagaaactac agagaaagaa atcgcaaaga aaagtaggaa tccacccgct aattttgcat
    11461 tcgcgctagt gtgcgaaacg tctttatctg aatcttgtgc tacagaatgt ttgtagaata
    11521 aaaatccaaa gagtgcccaa atcgctccaa tgaaaatcaa tcttccaata aaaaatggag
    11581 tattgagcca tccactttta tgttgaatca atttgtcgct cataacggct gcattatcag
    11641 tatgcgacca ttcatacaaa tcatgtgttc caaaaagaac taggattgca agcaacacaa
    11701 ctccaattgg caagaatttt gaataacttt ctgttattct tctcattgta acaagccagt
    11761 gtgcaccagt gatatgtcct atcgctgtga agaaaatccc agtgatggaa attcctaaaa
    11821 taaagaaggt tgcaactaga agcgcagacc atgctgggtt ggaatgtccg cccgcatgtc
    11881 ttgactcaaa gtgtagacca aaaaaaccgc caacaaaact tacgatccca attagaatca
    11941 taagaaagag tgtattacgt gttgcgccac ttaatttaaa attaatcaga ttatcttgaa
    12001 tttctgcttt cataatttac ctatttattg gcaggagctt ttgctttttg ctcagcttct
    12061 tgcaattttc taatgtataa aataatcttc catctgtcat ccggctcaat ttgtgccgca
    12121 taacttgcca ttaaacctct acccatagtg atgatatgat agatttgccc atcagtccag
    12181 ccttggattt ttgcaccaac tagacttgga ggacgaacag aaaatctagg atatggacct
    12241 acaacgttcc catttcctga tcctgcaaca ccatgacaag gagagcaaaa gatttggtat
    12301 ctactttcgc ctctctggta gttagcaaga gtagttttgg taggatatgg atttttcaat
    12361 ccttggtctt ttgtcatctc atctggagtt gctatatctg caaactcata tggatagtat
    12421 ccaactggaa tcgttccttc cggtggaagt cttcttccgt atgaattgtg gaaattatcc
    12481 agctcttgtg attctcttgc aggagagtca tacatatctg gaaaaaattc atgcaccggt
    12541 ttactatatt cacagttgct gattgcaact gctaagaaaa tgaaaataat aaatttcttt
    12601 agattcatgt ttaacctttt ttaactacgg tcacgtcagt cgcgcctaga gattttatga
    12661 aattaactgt atccgcttct ttgtaaccag gtgctgtagc aggaatccaa agagcaaatt
    12721 tatgactagt aatgtcccta tgtaatattt ttctacctac aactggaatt ctagataaga
    12781 aaaataaagc aagagcggag ctaataccag ccataaagat tgtcatctcg aaagtaactg
    12841 gaacaaatgc tggccaagca ttgtatgctt ttccagaaaa gttaatcggc cagtctactg
    12901 catgagtgag atattggtat ccgaaaccaa atgttcctcc aaccaaaccc atagccaatg
    12961 taatccaagg gattccagat ctaggaagac ccattgcatc atctaaacca tggacaggga
    13021 aaggagtcag gcaatcaaaa ccaacatagt attttttgtc tctagtctgt cttgctgctt
    13081 ctaaaatttg ctctggctca tcaaataaac caaaaacacc ttcagaagtt tctgtataag
    13141 aatgaaattg ttctaactta ggtaaataca ttttaatggt gacctccctt tgtaggcatt
    13201 atgctcttaa cctcagccgc agcaatcaca ggtaggattc ggcaaaagag taaaaagagt
    13261 gtaaagaata ttccaaatgt tcctatgagc atagcataat caaaaatagt tggggtatac
    13321 aatgcccagc tagatggtaa aaagtctcta tgtagagtca taacgattac aaaacgctca
    13381 aaccacatcc caatatttac aaaaatggaa actacaaaca ttacaggaat gcttgttcgc
    13441 atgcttttaa accagaacac ttgcggagaa agaacgttac aagaaaccat gatccagtat
    13501 gcccaccaat aaggaccaag aacacggttt acaaaagtaa aaccttcata ttcgtttcct
    13561 gaataccaag caatgaagaa ttctgttgca tacgcgtaac caaccataag accagttacc
    13621 atgatgactt tattcatatt ttccatatgc ttcattgtga tgtattcttt tagattaaac
    13681 atctcacgag caattaccat gagtgttacc accatagcaa atccagagaa gatagcaccc
    13741 gcaacgaaat aaggcggtaa aatcgtagta tgccaaccag ggagtaggga agctgcgaag
    13801 tcaaaactta cgattgtatg caccgagaga actagaggtg tagaaagtgc cgctaaaatc
    13861 atagaaacca tttctaaatg ggaccaagct ttgtttgatc cgacccatcc aaaagacatt
    13921 gcatcataaa tgagatattt gaacttattt gtagttctat cccttaccga cgcgatatca
    13981 ggaataagtc ctatatacca gaatacgagt gaaatactaa aatacgttga taccgcaaat
    14041 gtatcccaaa tgagcggaga tcggaagtta acccaaagag gtcctctttc gtttggataa
    14101 ggaaagagcc agaaacctaa ccaaggtcgt cctacgtgaa caatcaagga ggaagctgct
    14161 accattacgg caaaaattgt catagcttcc gccgcacggt taataccagt tctccattct
    14221 tgacggaaga ggtgtaaaac cgctgaaatc aaagttccag cgtgaccgat cccaatccaa
    14281 aatacgaagt taacgatgaa aaacccccaa ccaacaggat tgttaatccc aaggatataa
    14341 agcccttcat acattaaata tccgataatt cctaaatcta ccagagtaac ggttaaagct
    14401 aaaccaaaag cagtccacca aactttcgtt gggaaagact ctgttggctt taaaatatct
    14461 tccgtaactt gcttgtagct tttatttccc tctactagag gaacaatacc cagtttttct
    14521 tttattgcta ctgtcatcga catattatct tctaccttgc cttatacatt cactttgttt
    14581 ctaactctag ttaaataact aacttgtgga agaacattta aaaattctaa tattctataa
    14641 gctctgtggt catgagcatt cttagtaact tgtgcttctg gatcatttgt atttccaaat
    14701 gtaatcgcat ctgctggaca agtttcttga catgcagtct tgagctctcc gtcttttaaa
    14761 gttctacctt cattttttgc tttgattttt ttctcagcaa ttctagaaga acagaacgtg
    14821 catttctcca taacccctct gctacgaacg gtcacttcag gatttaaacc taagtgtctt
    14881 ggttctcttt ggtagtcaga gtctttccaa tgctgcatcc agttaaaacg acgaactttg
    14941 taagggcagt tgtttgaaca ataacgtgtt cccacacaac ggttgtaaac catatcgttt
    15001 aatccttctg aactatgtgt agttgcagca actggacaaa ctgtttcaca aggtgcattc
    15061 tcacaatgtt gacacattac aggctggtgt gcaatttcta aggattctgg ttttaatgga
    15121 tccccaatgt agtatcgatc aattcgaatc caatgcattt cacgaccaac tagaacttcg
    15181 ctctttccta caacaggaat attattctca acttggcaag ctgtgacaca agcagaacaa
    15241 ccagtgcaaa gacttaaatc gatcgatagt ccccatctat atcctttgta ttcaaactgt
    15301 gggttcaatc cgcgggacat ttctttttcg tttaattttg gaatttctga ttctgcttta
    15361 ccagattctg gattttttac ataatcttca aaagaggttg tttgaatcaa tggacggtca
    15421 tgttcatatg gtttatacaa accacctttt ggacccttac caggtgttgc ggtaggattc
    15481 atcatatggt gatcttgtgt ggtcgcaacc tttgaaacct taccggtttt ttctagggtg
    15541 actgtgattc ctgcatattg aactttacca tctactatgt ttgctagatt ataggaattt
    15601 ttacctacat ttccaacttt accagctttt tctcttccat aaccgagagc gatactaact
    15661 gtttcattgt gaagacctgg aacaatgagg gcaggaagag ttatctctct ggaacctgct
    15721 ttcactttaa ctacatcatt ggatttaatt ccaagagcat ccgcttgtcc tggagaaatt
    15781 cctaagaagt tttcccaagt agcttttgtt attggatccg gtagttcttg tagagtagga
    15841 ttatttgcgt gtgttccatc tccgattgca acggttgtat atagggagag agtaagagat
    15901 tttccagatt cttttgctgg aagttttgca actgcactcg ctttaaaact tctacctgct
    15961 ttgtttgact tgatatcagc ttctttgaaa aggcttccat ttcttaagaa gtcttcccaa
    16021 ttaaacttgc tacctaattt cttagtgtaa gcagatttta gatactcata ataagaagga
    16081 gatgctccta atttgccacc tgcccaaaca atcaaagagt cttctaaaga tcttgtattg
    16141 taaattggtc ggattgctgg ttgagtgatc gaaacaactc ctttcacagc ttctctatct
    16201 ccccaagatt ctaggtagtg agtagttggt gctaggtatt tcgcaaaact agctgtttca
    16261 tctagtctat ctgccaaaga aacataaaga cctgcttttg caaatgcatc ttttaagttg
    16321 agagatggca attggtaaac tggatttgtt ccataaacaa atagaactcc tacttcccca
    16381 gcgtttaatt ctttttgtaa gcttgctaaa tttgcagagt agagatgagc tgattcactt
    16441 ctattgttta caggatcaat tgtctttcca tcgttatcta ggaaagagtt aattaaattc
    16501 acagcaattt gtaaatctac agcgtctgta gtttgagaag atgttccacc agctactact
    16561 aaggattctc ctttcgcagc taaaagatct tttgcaattt gttggattag ttttgcatct
    16621 actccaattt cagaggaaag actttctaga gtgtaaccag tagtcgcgcc gagtgcaact
    16681 gcgattgcca ttgcaaatct tctagcatcc cctgatttaa tcgctattct tttgtctgcg
    16741 ttagaaccgg ttacagatgg aacagattct actgcataga atttattgat tttagagtta
    16801 acatctttaa tttttcttct agatgcgaat tgtttagaat attcaacttg agaaatccat
    16861 gttccgagga aatcagcatc gatcgaaaga attacctttg ctttatcaaa gtgatagttt
    16921 ggaactactg gttttccata agactcgttt gcagcaattg caatggattc ttcaacggag
    16981 gttacatcga tttcatagtg ctttccacca ccaacggcgg ttaaaaactc tgcaatcaca
    17041 gctttggtag aaggagagtc aataggacct gttacgattc tagtttttcc tttgagagct
    17101 gcaagttggg ttttcacttg tgtatctaga tcagcccatt tgattgtaac ggctgctcca
    17161 ttttttactt cagctggttc ttgtgctcta tctggatcgt ataaatctaa gatagaagac
    17221 tgaccaacag cacaaagtgc ccctttagaa attgggtttg caggatttcc ttctagtttt
    17281 aatggtcttg catctcttgt tttaaccaaa ataccgcaac cagcagaaca tccaccacat
    17341 gtagaagcgt agtaggttga ttgacctggc ttcacatagt caggagcctt tacataagga
    17401 acgattttct cttgtggttt ttggatacaa ttgagtgtaa gcatggcaga ggaagcaccc
    17461 atcaatttta ggaaggttct tctgtcataa tcccctgtct tgattcttga aatgattgga
    17521 tcaggagaag tataaaattc ttgattttgg atttgtttta gatcgctttt atcttttgtt
    17581 tcgtaagatt gccaatgtga ttttctttct tcttggaagc tgttgttaga catttactaa
    17641 taactccttt taaattatcg gtggcaagtg gagcagtcat ttggagcgtt gttctctcta
    17701 tgacaattca cacagtatcc catgttcaat gattcaactt gttttacttt taccatttcg
    17761 gcaacattgc catggcattt agaacaatct acaccgcggg aaatatggcg ggagtgattg
    17821 aagtaaacaa aatctggttg gtcatgaacc ttaatccatt ggataggttt accttttcct
    17881 tcgatggaat ttccagttga tttttcaccc tcaaactgct tagtgagcca ttttacgtcg
    17941 tttgttttgt ttgccacgtt agagtgacaa ttcatacaag tcgccgtagg cggaacactt
    18001 gcgtgtgcgg aagtttcaac actggtatga caataacgac aatcgatctt attgtctcca
    18061 gcgtgaatct tgtggttaaa gggaagaggt tggtccggcg catatcccac ataacgagaa
    18121 ggggaaaaaa tcaaatacgc tacggcagca accgtgataa gtggcattgt gatcttaata
    18181 gcttgtatat tcattctaaa gagaatcctt tcatgtccca aagagtaact ttttaatatg
    18241 attcggtaac attatatcta ccattctaca ctcttttttt tactatgaaa aatttcaaac
    18301 ggttttagta aattgtaaga ataaataaat ttccaagatg ggtagaggaa aagattacca
    18361 ccgatttacg ccgaagaaca cggataaata gattcgatat ttgatcttac tgattgggtt
    18421 tttgattttg ttttggtttc attgataaaa aaatctgaag gcgagtctac tgaggtttat
    18481 gcagtcggtc atcgagtggt gtcgagatga gcctactcca aagagttgtt atttctataa
    18541 actcaatgtt ttaatcttac cttatatcga cagctaagta taaactccaa acaggaaagg
    18601 atgcaaagta gtggaaaatc ataaaccatt cgcgctcttc gtgcccttcg tggttttaaa
    18661 aaaactgata gcaaaagata ttctaccacg aaggacacga aggaacacga agttgggtta
    18721 gtag
//
LOCUS       DAOQOS010000108        18613 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_1496_length_18613_cov_5.156051, whole genome shotgun sequence.
ACCESSION   DAOQOS010000108 DAOQOS010000000
VERSION     DAOQOS010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 18613)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18613)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18613
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1496_length_18613_cov_5.156051"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..396)
                     /locus_tag="PLF66_19415"
     CDS             complement(<1..396)
                     /locus_tag="PLF66_19415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66269.1"
                     /translation="MFRWLFLIFFIFFQCKPALISPIAKEGFLDLSNWNFERDGKLKL
                     DGEWEFYWEHLYNSEEIHSIQEKPVFFPFPSVWNEFKYKDKTLSAKGYATFRLKLKLP
                     ANCPPLALRTREQSTAYAVFWNNRKLLSAG"
     gene            910..1089
                     /locus_tag="PLF66_19420"
     CDS             910..1089
                     /locus_tag="PLF66_19420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66270.1"
                     /translation="MEAIRKITKIKNNSLKLVDLEDFDNQIVKVIIFPVAKKKENPKK
                     KKVVKSPTNEFLKIN"
     gene            complement(1269..1610)
                     /locus_tag="PLF66_19425"
     CDS             complement(1269..1610)
                     /locus_tag="PLF66_19425"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004765542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HMZ66271.1"
                     /translation="MIRGEIWWVDLGIPFGSEFGFRRPVLVIQDDSFNESNINTIIVV
                     AITSNLDLAEAPGNVILSKKESNLRKDSVTNVSQIVTLDRERFLSRIGKIRSSKMTQI
                     ENGIKLVLGLD"
     gene            complement(1597..1845)
                     /locus_tag="PLF66_19430"
     CDS             complement(1597..1845)
                     /locus_tag="PLF66_19430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010576493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChpI protein"
                     /protein_id="HMZ66272.1"
                     /translation="MKTAISIPDDLFKTAEKAAKRIGIPRSQLFARSLEEFIQSHNKS
                     SVTEKLNKVYRKDSRKSKEDISNSSVEFLRKSLKNDTW"
     gene            2268..2969
                     /locus_tag="PLF66_19435"
     CDS             2268..2969
                     /locus_tag="PLF66_19435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66273.1"
                     /translation="MGILSYKADLRSIGTSIETWDTASLGVLTGNAEIFHLAHSWISI
                     LIAKGYRVHVIDCAIRFNVYRVIDEANFYGLDLHEFLMTGLVQRAFTPYQILDVIRRI
                     LSSTKDPNRVYVLLAPTKQFFDGDVKEEEGYFLLQKLITVLARIQSKGIPMLAVESQK
                     YKHKTFQLLFPYLLDSANAIWKYNKLVFGKQTFRRFEINYKNTVVGPTQYRLEITGDE
                     NGKNGNALFESSRSY"
     gene            2923..3204
                     /locus_tag="PLF66_19440"
     CDS             2923..3204
                     /locus_tag="PLF66_19440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003001077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66274.1"
                     /translation="MGRTVMPYSRAVEATDLRLQDFRKSLRKDDREIFDELMRYAKTQ
                     IQAGVMAANPNPFDSMSLTMHIQLAKKLKNALAEIEKLKQELAKTKPES"
     gene            3360..5696
                     /locus_tag="PLF66_19445"
     CDS             3360..5696
                     /locus_tag="PLF66_19445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008594063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase domain-containing protein"
                     /protein_id="HMZ66275.1"
                     /translation="MKVLDHIQNHLPNSIQVVEGFLFDIYHVEEKVYVWVLDNEHKPL
                     LFMDTYYPEIYLDGRIDLMQKIVSRISFYNALKEKPVWETRRHFYSNKEVRVLKVVIS
                     KPSIMRKILNRLYAFYGLIDIYHTDIEVPTGYSYLHQIFPLAKVQIEYKKIENTNQII
                     SIHTKDDINACEYDVPSIKIMHLYLQKNHRMQIHSNNPVVADVDGDEFTFGFENPRKD
                     LEKLNQLLIEKDPDLILSNHGDQVLFPFLFHLAQSLKTKIYLDREDTTLHTRKIVRKG
                     TSYNTYGAMIYRAPSYPLFGRWHIDRGNSFVFKEAELRGIIELARISRLPMQRMARAS
                     TGNALTAIETNVALHGNYLVPWQKSKVEESKTFYDLLRIDKGGLVFQPDTANGIVFEN
                     MAQLDFSQMYPSIMVLHNLSPETVLCECCLNEKEIERVPGAGYHICKKRRGVVSEALE
                     KILSRRKYYKSRKKEVSGEEKEIVEAKQNSLKWMLVTSFGYLGYRNAKFGRIESHESV
                     TAFGREFLLTAKEIAEEANYYLSHAITDCIFIQKEDGSALSNAELESLCEKISDKTGI
                     SMAIEGVYKWLVYPPSKMDPLLPVSNRYFGKFQNGEIKMRGLAARRKDLPKFVVEMQL
                     DLLHIMEEADTILELKKLHREMHSTFHKWKEILESGNVPWRKLLLRKTVSQPMEDYSV
                     DNGSLLSLKQLSKLDIEVEPGEKIRYIVLKEKHLDKTKRYLSEEMAELTLVSDYVRYD
                     CNYYTRLIWDAFQEIWENFAPSGYFRFTPSRQIFFDFV"
     gene            complement(5737..7440)
                     /locus_tag="PLF66_19450"
     CDS             complement(5737..7440)
                     /locus_tag="PLF66_19450"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HMZ66276.1"
                     /translation="MAIKRNGKRESDVIFRRSYEEKSLEVEKKVNRIRYIFILIFYFL
                     ALSTYRNGSSPAVYITLFIIPSIQLVFALFWEYLLPRTKYSRTIKYISTTIDLLGVFG
                     AKYGMHFDPNAGWGLAMKEPATFDIFFLYICLAGLRLDKIFSLFTGFFAAALYLLLII
                     LSIGSGWMTFTNDPAKFFDFHFLRLPTEIAKILFLIGASFVIAYLANDTREFMGMLSE
                     SESKYKFNTSVMEKLLKKIEEISLSLTNMMSQLKDNTGSMEKTVKEQEEYFEKDSIVI
                     DKIVGDGEEISTISNAQLQMISKISSRTQKMSESIDQISKGGKESSKRATHAREISAE
                     SVNSLSDTMQVVNEMKSQSEKILNISQTINDIADRTNLLSLNASIEAARAGEQGRGFS
                     VVAMEVQKLAEQSLESSKEIHQIVNATVKNIEKSSNMIQATAKKLETVSNVVEENETF
                     LSELSESIKVQEKASFAINSDVKNITEIAENICALVEDEKKALNEFQIRNKNKTNLTL
                     DSVRTAESLHELSEVLGKISTELLTLIKNKEQVIAAEHRQPLKTFEEKVKDIRRDKPT
                     S"
     gene            complement(7450..9057)
                     /locus_tag="PLF66_19455"
     CDS             complement(7450..9057)
                     /locus_tag="PLF66_19455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017851264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HMZ66277.1"
                     /translation="MAVHVDRKKQLDRLSKTQFDCLIVGGGATGAGTAHDASLRGLNV
                     ALIDAKDFSSGTSSRSTKLIHGGVRYLAQFHFGLIQEALTERKRLLANAPHLVKPLKF
                     LMPSYKFYEKPYYSIGLTMYDLLAGDSGLPAHRRISKHEFLKEFPAAKETELRGGIAY
                     YDAQFNDSRLNVLLARTAEMEGACVCNKVQLTSLIKNESGKIVGAKVKDVTTDKEFTV
                     ATKLVINTTGVWIDEIRKMDDPNAKPVLSPSQGIHLVFPKSKIPCNSAMIIPKTSDGR
                     VVFLLPWEDHVVMGTTDTPIQTISHEPIPLKDEVEFLLKTGNEYLSSPLKKEDILSVF
                     VGLRPLISLSGSSDTKNISREEMILVSDSNLITMSGGKWSTYRKMAEDLTDKMIQVGK
                     LVPVRACETYNYNFIGKSGYSENMYLKIAQDYKLDLETAKRLRNYYGGEVFEILGKKP
                     KEILKDSGFFEEEVLHAVRSEFALSVTDIIARRMRILFINLNLAESMIKPVATILAKE
                     LKWTQTKKAKEEKEAKDLIQSLKETLN"
     gene            complement(9216..11351)
                     /locus_tag="PLF66_19460"
     CDS             complement(9216..11351)
                     /locus_tag="PLF66_19460"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HMZ66278.1"
                     /translation="MRYIFIILIILSQILLSDCKSKTSKPLPKALDGKLNLEEIDWNF
                     KTDGIVFLDGQWDFYWMRLLYPEDFQSEAKPVKSATVTVPGSWNGTIINQETLSGEGF
                     ATYKLTIPIDQSQVGQTLALKISYAATASKVWVNGNFISEDGKVGMSRKDMEPHYNSQ
                     IHSFRAENPIEIVIQVSNFNHKKGGLWQSLYLGLEEDIIKQNSSALFYDFFLFGVLCI
                     MTIYHIGLFILRREENTYLFFGLFCFIISVRILFTGETWITSLIPNINWDIQIKIEYT
                     TFYVSTQVFAGFVFYLYSAEFKRKIWILMNIIGTTFVGIVILTPPLFFTQTLPYFQIF
                     TLLCGFYSIYALILAIIRKRVGAIAACIGWTIFFIAIIGDIIINEMYGSSFLAPFGLF
                     IFIFSQSFILSLIFSRTFQKTQSLSIHLKSTNLAYSRFVPADFITFLNREDITQVLLG
                     DQTKTEMSVMFCDIRNFTALSETMKPEETFKFLNAYLKRVGPIIRKNNGFIDKFMGDG
                     IMALFPLNPEDAVRSAVEMQETVREYNLIRQKANYKPIQIGIGIHVGSLMLGTIGEAN
                     RMDATVISDAVNLASRLEGLTKIYGAPILISDVTFQKIKNQEDYQYRMLGRVQVKGKK
                     DSVGILEIIHDTSPEIADFKVTSKPMFERGIFYYLQKDFEAAANLFKNVAKANPQDKA
                     AELFLSRCEFYLKHGISEDWEGVEVFEIK"
     gene            11462..12676
                     /gene="tyrS"
                     /locus_tag="PLF66_19465"
     CDS             11462..12676
                     /gene="tyrS"
                     /locus_tag="PLF66_19465"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004424081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HMZ66279.1"
                     /translation="MNPTESFELIKRGTVEIIPESELKDKLNLNRKLVIKAGFDPTAP
                     DLHLGHAVLLRKMKHFQVLGHEVQFLLGDFTGMIGDPTGKSETRKRLTREDVVRNAET
                     YKEQVFKILDPSKTKIVFNSAWCSKMNFEDVLVLSSKYNVARLLERDDFSKRYKAGNP
                     ISLIEFLYPLVQGYDSVVMEADVELGGTDQKFNLLVGRELQRDYGKTPQTVITLPLLV
                     GLDGVKKMSKSLGNYIGFNEAPIDMFGKIMSISDELMWNYYELLTDLPKSEIENRKSA
                     IAKNEAHPKEVKTELAKLIMDQFHPSDSNQEAIEEWNRIHNTKNRAIPEDIETVILSD
                     DVFLSGQTQLLQVLAKQGFIPSTSEGRRLVKSGGLYLNEEKLSDEKFTVEKGKEYLVR
                     QGKKGKFLKIKT"
     gene            12803..13675
                     /locus_tag="PLF66_19470"
     CDS             12803..13675
                     /locus_tag="PLF66_19470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66280.1"
                     /translation="MKLKILLFILLVFSKILFSAEVTLRSETVFICDLLEENKDSILI
                     LYKDKKYKIPRSEILSVDPDVLGPHSSYKNTMVQLEDGSSTKGLFIEETKEILTIKKE
                     NNLIAIPRSKILQIQKGEANQSLPQEYQVLEQDKKENTWTIGVSATGFRNGRVVGDVH
                     RESFGFGFYVEPPFSLFNKLIQFGFQSEYFSTGGKLSYSFFQNMGYVLFTPNLFGYNL
                     YFKIGGGTSYVILSEGSASASVLKPVATIEGGIQNVFLERYIWRIGIRENGVYSHREF
                     LDMFGIQLSVGYRF"
     gene            13676..14683
                     /locus_tag="PLF66_19475"
     CDS             13676..14683
                     /locus_tag="PLF66_19475"
                     /inference="COORDINATES: protein motif:HMM:NF016041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HMZ66281.1"
                     /translation="MNYLQISLEIFAIPFKNIILTTGRLYWIYILSALLISFFYLFIR
                     EIIQGNFQIREFLKKIHFTNWKTKSSLTDYLYYFIETILHTIFFSHLIISSLATSIFI
                     FTILNEYGGEYRLAASLWVEILYTVLYLLAYDFSRFYIHKKMHDLSFLWEFHKFHHSA
                     TSLNYFTVYRVHPVESILLNSGSGIATGIVTGVFVYFYPSITMFQVLGVNLGLFLFQL
                     YGNLRHSETWISFPSFLNFILLSPASHQIHHSKDPKHYNKNMGVIFAFWDRIADTLYI
                     PQEKEKIQFGLPDENASDYRNLFFIFFKPFYKIVLTFVRYIEKINRLPPINTDKKYDR
                     S"
     gene            complement(15053..17425)
                     /locus_tag="PLF66_19480"
     CDS             complement(15053..17425)
                     /locus_tag="PLF66_19480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008591157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HMZ66282.1"
                     /translation="MLSTKNIFLLLILLFYSTILFSQESKTSPEPENSKSSETTEQLR
                     KKAEDFYSKGQINVIGDNKESLKKIPGSATVVSKKFLEETQPVDAMEILRRVPGASIR
                     FQDAAGLTPNIGFRGVSNEESRKTLILEDGILTSLSPYGQPESYYAPQIDRMQRVEVI
                     KGSGSILFGPSTIGGIVNFVTRKPPLKPTFSNKSIGGENGYMSNFAQYGGTFGKTGVD
                     VSYLYKQGDGFRNYQGFHLNDVSIKLVHEISEKHTITFKTGFQEQDAQSTYLGLTQGL
                     FWKNPKINPAQFDRKYLNRNSTVIGHEFTITDQMKLITRIYNNNAVRNWQREDFAYNN
                     INTNGVAASPPSDTFMTYSPGLIGVRPGDVIYMRNSAPLRNQNFATLGIESKLEIQLK
                     TGSIKHEIDFGVRAHGEKNRVSTEAVPLPFLKDGFPYSQQDRIAKTYAVYLHDRIALT
                     ERFKIMPGVRYEWIEQGVYNKRRYATTQDVRNRIATNVGDILFVNQGGESFTKVLIPG
                     FGMTYDITQTYTWFAGVHKGFSPPTYGTAVSPTGADYRLKAESSTNYETGLRGDITPY
                     LYTEFAVYILYFQNQIINTNEISGDAGSRPVNSGKSIHRGSENTITFDFGKFFNLSWN
                     IPLDLIYTYTNAKSLTYTPFPYTTNADGTINLLLNQPAYYFDEKFHLVNRDTNRNFLP
                     YVPLNVYTIALGANHKNGFYIRGEYQYIGRQFSDLANSPNESADGTRGIIPAVGLVNA
                     SLGYKHPVRKWSIFVTGKNLQDREYVSGRLPIGIQPGPFRQINFGISFEL"
     gene            complement(17428..>18613)
                     /locus_tag="PLF66_19485"
     CDS             complement(17428..>18613)
                     /locus_tag="PLF66_19485"
                     /inference="COORDINATES: protein motif:HMM:NF020932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="imelysin family protein"
                     /protein_id="HMZ66283.1"
                     /translation="MRKQNSFLFFILILNLFLWIQTCSNNKGSKESQDSAILGRFFTI
                     LQTYNPTPIFSNISNSIILTSYSNLESNARNLKTATDSYTQSCSGSTSVLNLQTLWKA
                     NMSALKDAEITQFGPAVQGGYYELVDPWVNSYLSNPADTGSINTYIASSNTISLSTIT
                     TLSKLQRGLPAIEYLLFDDGTGNTSLTAICNNLTGRRLQFLTQLVTDYYNNTNSLKNS
                     WISTNGNFVNELPTAGMGSSYFDSKKTALDTLINQMINLLNGIIDKKLGYPAGLNVTS
                     GGVIRSTFVESRYSDASVDNLVANLKSIRNYYTGNGGIGISDYVKNLNPILDRKILFQ
                     IEDAITKTQSISNLRTSLSSSNLTAATSAFNSIRNLRTTLSTELISLSGTSATTSTGD
                     GD"
BASE COUNT         5893 a         3097 c         3408 g         6215 t
ORIGIN      
        1 tccggcagag agtagtttgc gattgttcca aaaaacagca tatgcagtag actgctctct
       61 agttctaagg gcaagaggtg ggcaatttgc tggaagtttg agttttaaac gaaacgtagc
      121 atatcctttt gcggataacg ttttatcttt gtatttaaac tcattccata cacttggaaa
      181 aggaaaaaaa acaggctttt cctggataga atgaatttct tctgaattat agagatgttc
      241 ccagtaaaac tcccattccc cgtctagctt gagttttcca tctctttcaa aattccaatt
      301 gctcaggtca agaaagcctt cttttgcgat aggtgaaatc aaagcaggtt tgcattgaaa
      361 aaaaatgaaa aaaataagga atagccaacg aaacatggta ggactgttct aaaattttgc
      421 agaatctatg tcaagataaa ctctctacct cgacttggct cttaaaccta tcttgactcc
      481 gctcgataac cacctagact ttgattggta acctctcggc taataatttt aacttgtagg
      541 gagaaaagat taccaccgat tcacaccgat aaaagattcg atagggggta gggtaagacg
      601 ccaatttact ttgctgatgt ttgggggtga tattgaatat agaattaata gcaaatagac
      661 gttatctgct ttttggacta ttctatttct aataaaacct cgtattataa taaaatacta
      721 ttaagctttt acaaaatata tctaatgagt ttcaattggt tgcagttgtt tgccggtttc
      781 tttctgtcaa ttttaaaggg aatctaaatc atcaatgata gaatgatttt tccaaggaaa
      841 agacaaaacg atatctgtaa gaataagaat acttctggag taggaattga agattgttaa
      901 actgtaacta tggaagcgat tcgtaaaatt actaaaatta aaaataatag tctcaagcta
      961 gtagatttgg aagacttcga taatcaaatt gttaaggtaa ttatttttcc tgttgcaaag
     1021 aaaaaagaaa atcctaagaa aaagaaagtt gtaaagtctc caacaaatga atttttaaaa
     1081 ataaattgaa atccaagagc aaatgggcgt tatccgctct tgggaattga actttattta
     1141 agaatatttt aaccacggat taacacggat gcacacagat aaaggatgtg atggtatatt
     1201 tctagtgcct ttttatttgc aaatgaattt tatttcgcgc aggaagagta agcaatcgcg
     1261 ttatgtagtt aatccaatcc taaaacaagt ttaattccat tttcaatttg ggtcatttta
     1321 ctagatctaa tttttcctat gcgtgaaaga aaacgttctc gatctaaagt tacaatctgt
     1381 gaaacatttg tcactgaatc ttttcgtaaa ttggattctt ttttagagag aataacgttt
     1441 ccaggagcct ccgccaaatc caaattagac gttatcgcaa caacaatgat tgtgttgata
     1501 ttgctttcgt taaaagaatc atcctgaata acaagaacag gacgcctaaa gccgaactca
     1561 ctgccaaaag gtatacctaa atctacccac caaatttcac cacgtatcat tctttaaact
     1621 ctttctgaga aattcaactg atgaattaga aatatcttct tttgatttgc gagaatcttt
     1681 tctataaacc ttgtttaatt tttctgttac agaacttttg ttatgagact gaataaattc
     1741 ttctaaagac ctcgcaaaga gttggcttct aggaattccg attcgcttag ctgccttctc
     1801 ggctgtctta aataaatcat ctggaattga aatcgctgtt ttcattctct cagtataact
     1861 taggttatac cgaagtcact aataaaaatt aaattacgaa tctaaaaacc tagacatcat
     1921 tcattctaaa actaaaaatt cttttgacac cccaactcgt ttagaaaatg aattactgac
     1981 ctgaattaca ttgcctagtc tctcacttaa ttctgtcaac ttaaggattt ttaaccacat
     2041 agtttggctt atggtgagcg tcaagcgatg gggggaatgc aagaagactc acgagtgaat
     2101 aaataataaa tctttttttt cttcgtgttc tctgtgctcc tcgtggttaa ttttttttgg
     2161 tttctattaa gttgacagca tcgtctccca ctgttatgca cttatgtgtg acagcgaaca
     2221 attaaattta ctttacagat gtttatattg ttaaatacta aacaaatatg ggtatattga
     2281 gttataaagc tgaccttagg tcaattggca caagtatcga gacatgggat actgcaagcc
     2341 ttggggtatt aactgggaat gcagagattt ttcaccttgc tcatagttgg atttctattt
     2401 tgatcgcgaa aggatatcga gtgcatgtaa ttgattgtgc gattcgattt aatgtatata
     2461 gagtcattga cgaggcaaat ttttacgggc ttgatttaca tgagttttta atgacaggtc
     2521 tagtgcaaag agcctttact ccttatcaaa tcctagatgt gattcgaaga attttaagca
     2581 gcacgaaaga tccaaataga gtttacgtat tattagcccc tacaaagcaa ttttttgatg
     2641 gagatgtgaa agaagaggag ggatattttt tattacaaaa actaattaca gttctagcta
     2701 gaattcaatc taagggaata ccaatgttag ctgtagaatc tcaaaagtat aaacataaaa
     2761 catttcaact tttatttcct tatcttttag attccgccaa tgcaatttgg aaatacaata
     2821 aattagtttt tggtaaacaa acttttcgaa gatttgaaat caattataaa aatacagtag
     2881 tgggtccgac tcaatataga ttagaaatta caggagacga aaatgggaag aacggtaatg
     2941 ccttattcga gagcagtaga agctactgat ctaagattac aagattttag aaaaagcctt
     3001 agaaaagacg atagagaaat ctttgatgaa ttgatgcgat acgcgaagac tcagattcaa
     3061 gcaggagtga tggcagcaaa tccaaatcca tttgattcca tgtcacttac tatgcacatt
     3121 caactggcta aaaagttaaa aaatgcactg gctgaaattg aaaaattgaa acaggaatta
     3181 gctaaaacca aaccagagag ttaaaataga attaagaaaa aggattacca ctgatgaaga
     3241 cgataaacac cgataaaaga aaagatacga taattcccat cgtttctgca tcgttcagtt
     3301 atcggtgtcc tcggtggtaa ttcttaattt gacagtatta agtgtcgatc gcgagtgcta
     3361 tgaaagtttt agatcatata caaaatcatt tacccaattc gattcaagta gtggaagggt
     3421 ttttgtttga tatttaccat gtagaagaga aagtctatgt ctgggttctg gataatgagc
     3481 ataagccgct tctatttatg gatacctatt atcctgagat ttatctagat ggaagaattg
     3541 atcttatgca aaagattgta agtcgtattt ctttttacaa tgccttaaag gaaaagccag
     3601 tttgggagac tagaagacat ttttattcaa acaaagaagt gagagtttta aaagtagtga
     3661 tttctaaacc ttctatcatg cgaaaaattt taaatcgact ctatgcgttt tatggactga
     3721 tcgatattta tcatacagac attgaagttc ctacaggcta ttcttatttg catcaaatct
     3781 ttccactcgc aaaagtgcaa attgagtata aaaagataga aaatacaaat caaattataa
     3841 gcattcatac aaaggatgat ataaatgcat gtgagtatga cgttccctct attaaaatta
     3901 tgcatctcta tctacagaaa aatcatcgaa tgcagattca ttcgaataat ccagttgttg
     3961 cagatgtgga cggagatgaa tttacttttg gatttgaaaa tccaagaaag gatttagaaa
     4021 aactaaatca attgttaatt gaaaaagatc cagatctaat tctttccaat catggagatc
     4081 aggttttgtt tccattttta tttcatctag cacaaagctt aaagacaaaa atttacttag
     4141 atagagagga tactacctta cacactcgaa aaatcgttag aaagggaact agttataata
     4201 cctatggtgc tatgatctac cgtgctcctt cttatccact gtttggtaga tggcatatcg
     4261 atcgaggaaa tagttttgtt ttcaaagagg cggaattaag gggaatcatt gaacttgctc
     4321 gaatcagtcg acttcctatg cagagaatgg caagagcttc tacaggaaat gcgcttaccg
     4381 ctatagaaac taacgttgct cttcatggaa attatttagt tccatggcaa aaatcaaaag
     4441 tggaagagag taaaactttt tacgacttgc ttagaatcga taaaggaggg cttgtttttc
     4501 agcctgatac tgcaaatgga attgtattcg aaaatatggc acagcttgat ttttcccaga
     4561 tgtatccttc tattatggtt ttgcataatt tatctccaga aacagtttta tgcgaatgtt
     4621 gtttaaacga aaaagagatc gaaagagttc ctggggcagg ataccatatt tgcaaaaaga
     4681 gaagaggggt tgtttcggaa gccttagaga aaattttaag tcgtcgaaaa tattacaagt
     4741 ctagaaaaaa agaagtgagc ggagaggaaa aggaaattgt agaagcaaaa caaaatagtc
     4801 tcaagtggat gcttgtcact tcgtttggct acttaggtta tagaaatgca aaatttggaa
     4861 gaattgaaag tcatgagtcg gtaactgctt ttggaagaga atttttactc actgccaaag
     4921 agattgcaga agaggctaat tactatttgt ctcatgcgat tacagattgt atatttattc
     4981 aaaaggaaga tggatctgct ctttccaacg ctgaactaga atcgttatgt gaaaaaatat
     5041 ccgataaaac gggaatctca atggcgattg aaggggttta taaatggctt gtttatcctc
     5101 cttctaaaat ggatccatta ttgccggttt ccaatcgata cttcggaaag tttcaaaatg
     5161 gggaaattaa aatgagaggg cttgccgcta gacgaaaaga cctccctaaa tttgtagtag
     5221 agatgcaatt agatttactt catatcatgg aagaggctga tacaatttta gaattaaaaa
     5281 agctacacag ggaaatgcat tctacctttc acaaatggaa agaaatttta gagtctggta
     5341 atgttccttg gagaaaatta cttcttcgaa agacagtcag tcaaccgatg gaggattatt
     5401 cagtagacaa tggaagtctg ctctctttaa agcaactcag taagctagat atagaagtag
     5461 aacctggtga aaaaattaga tatattgtat taaaagaaaa gcatttagat aaaaccaaac
     5521 gatacctatc agaagaaatg gcagagctaa ctcttgtatc tgactatgta agatacgatt
     5581 gcaattatta tacaagactc atttgggatg cctttcaaga aatatgggag aattttgccc
     5641 caagcggtta ctttcgattt acccctagta ggcaaatatt ctttgatttt gtttagacct
     5701 agttaagaat tcgctttttc gatttaaact aattctttag ctggttggtt tatctcttcg
     5761 aatgtctttt actttttctt cgaatgtttt gagtggttgt ctatgttctg ctgcaatcac
     5821 ttgttctttg ttttttatca aagttaaaag ttcagttgaa atttttccca acacttcgct
     5881 caattcgtgc aaggattctg ccgttcgaac agaatctaat gttagatttg ttttgttctt
     5941 atttctaatt tgaaattcgt ttaatgcttt cttttcatct tctacgagag cacaaatatt
     6001 ctctgcaatt tctgtgatat tttttacatc actattgatt gcaaaactcg ccttttcttg
     6061 gactttaata ctttcactta gctcacttag gaaagtttca ttttcttcta caacatttga
     6121 aacagtctct aatttctttg ccgttgcctg aatcatattc gagcttttct caatattttt
     6181 gacagttgca ttcacaattt gatggatttc ttttgatgat tccaaacttt gctctgctaa
     6241 tttttggact tccattgcga cgaccgaaaa tcctcttcct tgttctccag cccttgctgc
     6301 ttctatcgat gcattgagag ataaaagatt tgttctatct gcaatatcat tgattgtttg
     6361 agagatgttt aatatttttt ctgattggga tttcatttca tttactactt gcatagtatc
     6421 acttaaggag ttaacgctct ctgctgaaat ttcccttgca tgtgtggctc gtttggaaga
     6481 ttcctttcct ccttttgata tttgatcaat cgattccgac attttttggg ttctacttga
     6541 aatctttgag atcatttgca attgtgcatt tgagatagtg ctaatttctt ctccatcccc
     6601 aacaatttta tcgattacaa tagagtcttt ttcaaaatac tcttcttgct ctttaactgt
     6661 tttctccatg ctgcctgtat tgtcttttaa ttgagacatc atattcgtaa ggctaagtga
     6721 gatttcttcg atcttcttta aaagcttttc cattacagag gtattgaatt tatacttgga
     6781 ttctgattca gaaagcattc ccataaattc tcttgtgtca tttgctagat aggcgattac
     6841 aaaagaggct ccgattaaaa aaagaatctt cgcaatttct gttggtaatc ttaaaaaatg
     6901 aaaatcaaaa aactttgcag gatcatttgt aaatgtcatc caacctgatc caatcgacaa
     6961 gatgattagt aatagataga gtgccgccgc gaaaaatcct gtaaaaagag aaaatatttt
     7021 atctagtctg agaccagcca gacaaatgta taagaaaaat atgtcaaatg ttgcaggttc
     7081 tttcattgcg agtccccatc ctgcatttgg atcaaaatgc attccatact tggcaccaaa
     7141 tacacctagc aaatcaatag ttgtagagat gtatttgatt gttctagaat actttgttcg
     7201 tgggagtaaa tattcccaaa aaagtgcaaa tactaattga atacttggaa tgataaaaag
     7261 cgttatataa acagcagggc tacttccgtt tcgataggtg gagagagcaa ggaagtaaaa
     7321 aatgagaata aaaatatatc gaattcgatt tacttttttc tcgacttcta aacttttctc
     7381 ttcataggat ctacgaaata taacatctga ttctcgtttg ccgtttcttt ttatagccac
     7441 tctatactcc tagtttaaag tttctttcaa tgactggatt aagtctttcg cctctttttc
     7501 ttcttttgct ttttttgtct gtgtccattt taattctttt gctaaaatgg ttgcgaccgg
     7561 ttttatcatt gattctgcta aattcagatt tataaaaaga attctcattc ttcttgcaat
     7621 gatatctgta acggataacg caaactcact acgcactgca tgtaatactt cttcttcaaa
     7681 gaatccagaa tcttttagaa tttcttttgg ctttttacca agaatttcaa aaacttctcc
     7741 tccataatag ttcctgaggc gctttgcagt ttctaggtct agtttatagt cctgagcaat
     7801 tttgagatac atattttctg aataaccact ctttccaatg aagttatagt tgtaagtctc
     7861 acaggctcgc actggaacta actttcctac ttggatcatt ttgtccgtta agtcttcagc
     7921 cattttgcga taggttgacc actttccgcc actcatagta attagattgg agtcagaaac
     7981 taaaatcatt tcttctctag aaatattttt agtatcagaa ctaccagata gagaaattaa
     8041 cggtctgagt cctacaaaca cagaaagtat atcttctttc tttagtgggg acgataggta
     8101 ttcatttcct gtctttaaaa gaaactctac ttcgtctttt aatggaattg gttcatgaga
     8161 aattgtttgg ataggtgtgt ctgtggttcc cattactaca tggtcttccc aaggcaatag
     8221 aaatacaact ctgccatcac ttgtctttgg aatgatcatt gcgctattgc aaggaatttt
     8281 tgattttgga aagactaagt gaatcccttg gctgggggat aatacaggtt ttgcgttcgg
     8341 gtcatccatt tttcgaattt catcaatcca aacaccagtc gtattaatga caagcttggt
     8401 tgcgactgta aattctttgt ctgtcgttac atcttttact tttgctccta caatttttcc
     8461 tgattcattt tttatgagag aagtcaattg cactttatta caaacacaag ctccttccat
     8521 ttctgcagtt ctcgcaagta aaacattgag ccgagaatca ttgaattggg catcgtagta
     8581 ggcgattccg cctcttagtt ccgtttcctt tgccgctgga aattctttta aaaattcatg
     8641 ctttgaaatg cgtcgatgcg caggcaaacc actgtcaccc gctaaaagat catacatggt
     8701 tagaccgatt gaataataag gcttttcata aaacttgtag ctaggcatta aaaatttcag
     8761 tggtttcaca aggtgaggag catttgctag aagtcttttt ctttcggtta acgcttcttg
     8821 aattaagcca aaatgaaatt gcgcgagata acgaacccct ccatgaatta acttggtaga
     8881 tcggctcgag gttccggaag aaaaatcctt ggcatcaatt aacgctacat tcaaacctct
     8941 taaagaggcg tcgtgtgcag tcccagctcc agtagcacca cctccaacaa tgagacaatc
     9001 gaattgtgtt ttactaagtc tatctaattg tttttttcta tcaacatgca ctgccatttt
     9061 attaaagttc ctaattcaaa attctataac taaaaaatcg ctttgttttt tagtttattt
     9121 gcattattct gagtgaatca aaaaactaaa tccttttttt taaaaagaga aatcattttt
     9181 aaactactag gctatactag attgcaggaa aagaattact ttatttcaaa gacttccaca
     9241 ccttcccaat cttcgctgat cccatgtttt agatagaatt cgcatctaga tagaaaaagt
     9301 tcagccgcct tatcttgtgg gtttgccttt gctacattct taaaaagatt tgcagccgct
     9361 tcaaaatcct tctgtaaata gtagaagatt cctctttcaa acattggttt agatgtgact
     9421 ttaaaatcag caatttctgg tgaagtgtca tgaatgattt ctagaattcc tacagaatct
     9481 tttttgcctt ttacttggac cctaccgagc atcctatatt gatagtcttc ttggttctta
     9541 attttttgga atgttacatc gctaattaaa attggtgcgc cataaatttt agtaagacct
     9601 tccaaacgag aggcgagatt aactgcatct gaaatcactg ttgcatccat tcgattcgct
     9661 tctccaatcg ttccgagcat aagacttcca acatgaattc caattccaat ttggattggt
     9721 ttataatttg ctttctgacg tattagattg tattcccgaa ctgtttcttg catttcaact
     9781 gcagatcgga cagcatcttc tggattgagt ggaaagagtg ccataattcc atcacccata
     9841 aatttatcga tgaagccatt gttcttacga atgattggtc ctactcgttt tagatatgcg
     9901 tttaaaaatt tgaatgtttc ctctggcttc attgtttcgg ataacgctgt aaaattgcga
     9961 atatcgcaaa acataacaga catttctgtt tttgtttggt ctcctagtaa tacttgtgta
    10021 atatcttctc tgtttaaaaa agtgataaaa tctgcgggaa caaatcgact gtaagcaagg
    10081 tttgtagatt tgagatggat cgataaagat tgagtctttt gaaaggttct ggaaaaaatc
    10141 aaagagagta taaaagactg agagaaaata aaaataaata aaccaaacgg agcaagaaaa
    10201 ctagatccat acatttcatt gattataata tcaccaatga ttgcaataaa gaagatcgtc
    10261 caaccaatac aagctgcaat ggctccaacg cgcttgcgga taatggctaa tatcaatgca
    10321 tagatagaat agaatccaca taagagagta aaaatctgga aatagggaag agtctgagta
    10381 aaaaataagg gaggagttaa aatcactatt ccaacaaatg tggttccgat tatattcatt
    10441 agaatccaga tttttctttt gaattctgca gaatagagat agaaaacaaa tccagcaaag
    10501 acttgggtgc ttacataaaa agtagtgtat tcaattttga tttgtatgtc ccagtttata
    10561 tttggaatta aagatgtgat ccaagtttct cctgtgaata gaatccgaac tgaaataata
    10621 aaacaaaaca atccgaaaaa caaataggta ttttcttctc ttcgtaaaat aaacaaaccg
    10681 atatggtaaa tcgtcatgat acaaaggaca ccaaataaaa agaagtcata gaaaagggca
    10741 ctgctgtttt gtttgataat gtcttcttcc agtccgagat aaagactttg ccaaagacca
    10801 ccttttttat gattgaaatt ggatacttgg attacgattt cgattggatt ttctgcgcga
    10861 aagctatgta tctgactgtt gtaatgaggt tccatgtctt ttctagacat tcctactttt
    10921 ccatcttctg aaatgaaatt tccgttgacc cagacctttg aggcggttgc agcatagcta
    10981 atttttagag cgagagtttg tcctacttgc gattgatcaa taggaatggt aagtttgtag
    11041 gtagcaaagc cttcgcccga aagagtttct tggttgataa tggttccatt ccagctacct
    11101 ggaactgtta ccgttgcaga ttttactggt tttgcttcac tttggaaatc ttctggatat
    11161 aagagtcgca tccagtaaaa gtcccattga ccatctaaaa aaacaattcc atctgtttta
    11221 aagttccaat ctatttcttc caaatttagt tttccatcca gtgctttggg gagaggcttg
    11281 gaggtctttg atttacagtc actcaagagg atctgtgaaa gtataattag aattataaaa
    11341 atatatctca tgcaaactca attaaaatct aagaatagtc ttaattaaag gattacttag
    11401 aataatcaat caataaaaat tagtttattt aggagctcaa aaaaaaatag agtcttgtta
    11461 tatgaatccg acagaaagtt ttgaattgat taaacgggga acggtggaga tcataccgga
    11521 atctgaatta aaagacaaat tgaatttaaa tcgcaaattg gttataaaag ctggtttcga
    11581 tcctacagca ccggacctac atttaggtca tgcagtgctt cttagaaaga tgaaacactt
    11641 tcaagtttta ggtcacgaag tccaattttt actgggtgat tttactggta tgataggaga
    11701 ccctacaggc aaatcagaga cacgtaaacg tttgactaga gaagacgttg ttcgaaatgc
    11761 tgaaacttac aaagaacaag tttttaaaat attagatccg agcaaaacaa agatcgtttt
    11821 taattccgct tggtgttcta aaatgaattt tgaagatgtc ttagtcttaa gttctaaata
    11881 caatgtagca aggcttttag aaagagatga ttttagtaaa cgatataaag caggaaatcc
    11941 gatttcactt attgaatttt tatatccgct cgtgcaaggt tatgactctg ttgtgatgga
    12001 ggctgacgta gaattgggtg ggacagatca gaaattcaat ttactagtag gtcgtgaatt
    12061 acaaagagat tatggaaaga cacctcaaac tgtaattact cttcctctcc ttgttggttt
    12121 ggacggagta aagaaaatgt ctaaatcact tggaaattat attggattca atgaagctcc
    12181 tatcgatatg tttggaaaaa tcatgtccat ctcagatgag ctgatgtgga attattatga
    12241 gttattaacc gatttaccaa agtctgagat cgaaaatcga aaatctgcca ttgcgaagaa
    12301 tgaggctcat cctaaggaag taaaaacaga actggcaaaa ttgattatgg atcaatttca
    12361 tccttcagat tcgaatcaag aagcaatcga ggaatggaat cgaattcaca atactaaaaa
    12421 tagagccatt ccagaagaca ttgaaacggt aattctatca gatgatgtct ttctttctgg
    12481 gcaaacacag ctacttcagg ttctggcaaa gcaaggattt atcccatcca catcggaagg
    12541 tagaagattg gttaaaagtg gcgggctcta tttaaacgaa gaaaaactga gtgatgagaa
    12601 gtttactgta gaaaaaggga aagagtattt agtcagacag ggcaaaaaag gaaaattttt
    12661 gaagatcaag acttaaaatt tcttgacaaa gatttttttt tgatataatg accctgttca
    12721 tttctattag tcgatttgtt ttttggaatt ctaaattgag ataccaagtt agaattccac
    12781 aattgcaaaa aaatgaaaag ttatgaaact aaaaattctc ctgtttattc tccttgtttt
    12841 tagtaaaatt cttttttccg ccgaagtgac ccttcggtcg gaaactgttt ttatctgtga
    12901 tctactagaa gaaaataaag attcaattct aattctatac aaagataaaa aatataaaat
    12961 tccgcgcagc gaaatacttt ctgtcgatcc ggacgtttta ggtccacata gctcttataa
    13021 aaatacaatg gttcaattgg aagatggaag ttctaccaaa ggactcttta tagaagaaac
    13081 gaaagagatt ctaacaatta aaaaagagaa caatttaatt gcgattcccc gatctaagat
    13141 tcttcaaatt caaaagggag aggcaaatca aagtttacca caagaatacc aagtgttaga
    13201 acaagataaa aaagaaaaca cttggacgat aggtgtttct gcgacggggt ttagaaacgg
    13261 aagagtggta ggggatgtgc atagagaaag ttttggattt ggtttttatg tagaaccacc
    13321 ttttagtctt tttaataaac tgatccagtt tgggtttcag tctgaatatt tttctactgg
    13381 tggaaagtta tcttattctt tttttcaaaa tatgggatat gttttattta ctcccaattt
    13441 attcggatac aatctatact ttaagattgg aggcggaaca tcttatgtaa ttctttcgga
    13501 agggagtgca tctgcttctg tattaaaacc agttgcgacg attgagggtg gaatccaaaa
    13561 tgttttttta gaaagatata tttggagaat tggaattcgg gagaatggag tttattcgca
    13621 cagagaattc ttagatatgt ttggaataca gttgagtgta gggtatcgtt tctagatgaa
    13681 ctatttacag attagtttag aaatttttgc cattccattt aaaaatataa ttctaacgac
    13741 ggggagactg tattggattt atattttatc agcacttctg attagctttt tctatttatt
    13801 cattagagaa atcatccaag gcaattttca aattcgtgag tttcttaaaa aaattcattt
    13861 tacaaattgg aaaaccaagt catctcttac ggattatctt tattatttca ttgaaacaat
    13921 tttacataca atcttctttt ctcatttgat tatatctagt ctagctactt ctatttttat
    13981 ttttacaatt ctaaatgagt atggaggcga atatagatta gccgcaagtc tttgggtgga
    14041 gattttgtat acagtcttat atttactcgc ctatgatttt tctcggtttt atatacataa
    14101 gaaaatgcat gatctttcct ttttatggga gtttcataaa tttcatcact ctgctactag
    14161 tttaaactat tttacagtgt atcgggttca tcctgtagag agtatcttac tcaattcagg
    14221 aagtgggatt gcgaccggca ttgtgacagg agtttttgtt tatttttatc catctattac
    14281 tatgtttcaa gttctaggag tgaacctagg acttttttta tttcaactgt atgggaattt
    14341 aaggcacagt gaaacttgga tttcttttcc tagtttttta aattttattt tattaagtcc
    14401 agcgagtcac caaattcatc atagcaaaga tccaaaacat tataataaaa atatgggagt
    14461 gatctttgct ttttgggata ggattgcgga tactctttat ataccacaag agaaagaaaa
    14521 aattcaattt gggcttccag acgaaaatgc aagtgattat agaaatctat tctttatttt
    14581 ctttaaacca ttttataaaa ttgttttaac attcgttcgc tatattgaaa aaattaacag
    14641 attgccaccg ataaacactg ataaaaaata cgatagatct taattaactc atcttacaca
    14701 caagagccta agatgagaga cagggagcct gtcggcaaca cttcgactag ggctcagtgc
    14761 atcgcttggg ctagccgccc aaaccctttt atttattgat ccattaggaa aacgtaatat
    14821 gctaaccttt attattatcc ctataaattt ctatcctttg cgtaaggtga gttaattaac
    14881 gtgaattcga gataaaaaat ttttcaccac cgatcgccga atgatacaga aacaattttg
    14941 aattatcgta tcctatatcg gtgtcctcga tgattatcgg tggtagtctt ttcaagtcta
    15001 aaaacgttac gcctcgactt cgctcgccga ccgatatagg ctttattatc aactaaagtt
    15061 cgaaactgat tccgaagttt atctgcctaa atggtccggg ctgtattcca ataggaagtc
    15121 ttccagaaac atattctcta tcttgtaggt ttttacctgt gacaaagatc gaccattttc
    15181 ttacaggatg tttatatccc aaagaagcat ttactaaacc aacagccgga atgattccgc
    15241 gagttccatc tgcactttca ttcggagaat ttgctaggtc agagaattgt cttccaatgt
    15301 attggtattc ccctcgaata tagaatccgt ttttatgatt tgcaccgaga gcaattgtat
    15361 agacgtttag tggaacatag ggaagaaaat ttctgtttgt gtctcgattg actagatgaa
    15421 atttttcatc aaaataataa gcaggttggt ttaaaagcaa gttgattgtt ccatctgcat
    15481 ttgttgtata cggaaaggga gtatacgtaa gtgattttgc atttgtatac gtatagatta
    15541 aatcaagagg gatgttccaa cttaggttaa agaattttcc aaagtcaaag gtgattgtat
    15601 tttcacttcc tctgtgaatt gatttaccac tattgaccgg tctagatcca gcatcaccac
    15661 tgatttcatt tgtattgata atctgatttt gaaagtataa aatataaact gcaaattcgg
    15721 tatagaggta gggagtaata tcacctctta aacctgtttc ataattggtg gaactttctg
    15781 cctttagtct ataatctgcc cctgtgggac taacggctgt tccgtaggta ggaggagaga
    15841 atcctttgtg aacacccgca aaccaagtat aagtttgagt tatatcatag gtcataccaa
    15901 agcctggtat taacaccttt gtaaaacttt ctccaccttg gtttacaaat aatatatcac
    15961 caacgttagt agcgatacgg tttctaacat cttgcgttgt tgcgtatctt cgtttattgt
    16021 ataccccttg ttctatccat tcataacgca ctcctggcat gattttaaat cgttcggtca
    16081 gtgcgattct atcatggaga tagactgcat aggtttttgc aattctgtct tgctggctgt
    16141 aggggaatcc atcttttaaa aatgggagtg gaacagcttc tgtgctgact ctattttttt
    16201 ctccgtgtgc tctcactcca aaatcgattt catgtttgat gctacctgtt ttgagttgga
    16261 tttctaattt agattcaata cctagagttg caaagttttg atttctaaga ggagcagagt
    16321 ttcgcatata aatcacatct ccgggtctaa caccgattag cccaggcgag taggtcataa
    16381 atgtatctga tggaggactt gcggcaaccc catttgtatt tatattatta taagcaaagt
    16441 cttctctttg ccaatttctg actgcattgt tattatagat tcttgtgatt aatttcatct
    16501 gatctgtaat tgtaaattca tgtccaatta cagtagaatt tcgatttaag tatttacgat
    16561 cgaattgcgc aggattaatt ttaggatttt tccaaaacaa accttgggtg agtcctagat
    16621 aggtagattg tgcatcctgt tcttggaatc ctgttttaaa tgtgatggta tgtttttctg
    16681 aaatttcatg gactagttta attgatacat catttaaatg gaaaccttgg taatttctaa
    16741 atccatctcc ttgtttatag agataagaaa cgtcaactcc agtcttacca aaggttccgc
    16801 catactgagc gaagttagac atatagccat tttcccctcc aatgctttta ttggagaagg
    16861 ttggttttaa aggaggtttg cgagttacaa agtttacaat tccaccgata gtagatggac
    16921 caaataaaat ggaaccagaa cctttgatca cttcaactct ttgcattcta tctatttgcg
    16981 gagcatagta actttctggc tgtccatagg ggctaagcga tgttaggatt ccatcttcta
    17041 gaatcaaggt ctttcttgac tcttcattac tgactcctct aaatccaata ttaggagtaa
    17101 gtcctgctgc atcttgaaag cgaatggaag ctccaggaac gcggcgtaaa atttccattg
    17161 catctaccgg ttgtgtttct tctaagaatt ttttagaaac aacagtagca gatccaggaa
    17221 ttttttttag agattccttg ttatctccga tcacattgat ttgtcctttc gagtaaaagt
    17281 cttccgcttt ttttctcaat tgctcagttg tttctgagga tttagaattt tctggttcag
    17341 gagaagtttt gctttcttgg gagaatagga tggtagaata gaaaagaagg attagaagga
    17401 gaaaaatatt ttttgtggat aacatcatta atccccatca ccagtgcttg tagtcgcact
    17461 tgttcctgaa agagagatca attcagtaga gagagttgtg cgaagatttc taattgagtt
    17521 aaaggcagat gtggctgctg tcaaatttga actagaaaga gatgttctta gattggaaat
    17581 agactgagtt tttgtgatag catcttcgat ttgaaatagt attttcctat ctagaattgg
    17641 atttaaattt ttaacatagt ctgagattcc aatgccgcca ttgcctgtat aataatttcg
    17701 aattgatttt aaattggcaa ctaaattgtc tacagatgca tctgagtatc tagattcgac
    17761 gaaggtagat cggatgactc cgccggatgt tacattgaga cctgctggat aaccaagttt
    17821 tttatcgatg attccattta atagattgat catttgattg attagagtat cgagagcggt
    17881 ctttttagaa tcaaaataac tacttcccat tcctgctgtt ggaagttcat tcacaaaatt
    17941 accgttagta gaaatccaag aatttttaag tgagtttgta ttgttgtaat agtcggtaac
    18001 gagctgagtt aaaaattgta aacgcctacc tgtgagatta ttacaaatag cagttaagga
    18061 tgtgtttcct gttccatcat caaagagtag gtattcaata gctggtagtc ctctttgtaa
    18121 tttgcttaag gtagtgattg tagaaagact aattgtattc gagctagcta tataagtatt
    18181 gatagatcct gtatctgctg ggttagaaag ataggaattg acccagggat cgactaactc
    18241 atagtaaccg ccttgaacag ctggaccaaa ttgcgtaatc tctgcatcct ttaaagcaga
    18301 catatttgcc ttccaaagag tttgtagatt taaaacggag gttgagccag aacaactctg
    18361 cgtataacta tccgtagccg tctttaggtt tcgggcattt gattctaaat tagaatagga
    18421 ggtaagtatg atagaattac ttatgttact aaaaatggga gttggattgt aggtttgcag
    18481 aattgtgaaa aatctaccta gaatagcaga atcttgactt tctttagagc ctttgttatt
    18541 ggagcaagtt tgaatccata aaaaaagatt taaaataaga atgaaaaata aaaaagaatt
    18601 ttgcttacgc ata
//
LOCUS       DAOQOS010000109        18543 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_1501_length_18543_cov_5.960623, whole genome shotgun sequence.
ACCESSION   DAOQOS010000109 DAOQOS010000000
VERSION     DAOQOS010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 18543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18543
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1501_length_18543_cov_5.960623"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            217..858
                     /locus_tag="PLF66_19490"
     CDS             217..858
                     /locus_tag="PLF66_19490"
                     /inference="COORDINATES: protein motif:HMM:NF019235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1564 family protein"
                     /protein_id="HMZ66284.1"
                     /translation="MNLDQDPNNQENSESQTTYNEFLSEFRKYLYEKFAGFQIESHKN
                     ASSISSQKIVFPHEDFKYSEFSSTLLIPERYYEIFRRGCWQHGGTRGYVAYLLYKYQL
                     FIANGLVPGYSKVTTKYQEKGQNLHKVAFRPRPEDWAELKLYRVSFGMSISAFLVYLL
                     IADSVDFAEAVSYYLTAVGISSIPNFDLCAKIFLTNNRSDYTIFFQFRKSKYG"
     gene            complement(977..1567)
                     /locus_tag="PLF66_19495"
     CDS             complement(977..1567)
                     /locus_tag="PLF66_19495"
                     /inference="COORDINATES: protein motif:HMM:NF016062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004671 - protein C-terminal
                     S-isoprenylcysteine carboxyl O-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006481 - C-terminal protein methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HMZ66285.1"
                     /translation="MFELNFGGFLFLSVWIVTIIFRLFELRLAKRNLAKRKADPNLKM
                     AREPFFFLFVVLHSSFLVLVPLEIFYFKRVFEITQGLFCLFIYLGCLGMRISVLTTLK
                     ENWNVKVVYDINSKNSIATTGLYKFIRHPNYLIVILEIATISLLHGAFYSFVFFSLCN
                     FSILYFRIKQEETALFQNPFYAEHFKNKKRFIPFIF"
     gene            complement(1567..2349)
                     /locus_tag="PLF66_19500"
     CDS             complement(1567..2349)
                     /locus_tag="PLF66_19500"
                     /inference="COORDINATES: protein motif:HMM:NF013955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HMZ66286.1"
                     /translation="MKGMRVKILFFILIIGIFHCKKDTAKTNPYASLLIGQSLYSPQI
                     FSVDPKRGNPPFTTPSSVGFIGASYNATTINIKGKNFIASTTGNTVSINGIQAIVDYA
                     GTDQLTFKVPSGASTGPITVANNGGACSNADRKSGINCDGDDFYIDCYGAFNNQFGSE
                     TAVGTGTSQTVTYDSLTTKAFRSDLIFTNPYSTDTAQNTITIRCANFTRVLLFSPSCV
                     PTEYTANGSNIVINPVISINSRFYTIQYLLTATKGDCTIRVN"
     gene            2456..3061
                     /locus_tag="PLF66_19505"
     CDS             2456..3061
                     /locus_tag="PLF66_19505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66287.1"
                     /translation="MKQLLAFLFIIPFLNLLSESQPLTNPNKADSKSTTKTVEAKTVD
                     AKSTETKPAEPKAPAKADTNVKIETPPKKELPPLQKEGSFYVYKGVKWQADNITQKAY
                     LSAKDSCEKEGMRLPTKDELLDAFQSKHVEFRTPAGAYITGNRVASDRSKIWYINFDN
                     GHHNHGSFQREFNVRCVKQEPPAKTEAKTEAKTEVKTEAKK"
     gene            3082..8700
                     /locus_tag="PLF66_19510"
     CDS             3082..8700
                     /locus_tag="PLF66_19510"
                     /inference="COORDINATES: protein motif:HMM:NF019323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HMZ66288.1"
                     /translation="MKKYLILSFLLLSNFFSLIAQEKNTITSFSPEGVVKNIKQVKVS
                     FSSQMVPFGNPRVKTDQFEINCPIEGKARWIDEKTFVYEFANNLEAGIQCKFQLKPEV
                     KTLSGKDISGNKTFSFSTGGPAILYTNPYEGNNIDEDQIFILTLDADVKESSLENKLA
                     FEIEGVKDRVAAQVIKPSDAQEILKSEFGERYKTDKLLLIKSKLRFPNKAKVSLIWGK
                     GIESKSGVKTDEDRVLTYSARPPFTAEFSCDRENKNAGCLPITNLYVNFTSPISKSDA
                     KKATLEGNGRKWNAKISGSNEEEDGTTYGIYFEGPFPEETKFKISIPESIKDDSGRKL
                     ANANKFPLEVKTDKYPPLAKFSGSFGIIELEAEPLLPVTLRNLEKDVKAKVLKMTGEG
                     GNFFSNLFGKKIKITPDNILIWLKKVSKAEREKSIFAGEVNTNSFNIPKPNGEKAFEV
                     VGIPLKEPGFFVVELESEKLGNSLLEKKGKMYVPTSALVTNMAVHFQWGRESSLVWVT
                     SLSKGEPISGAKISIRDCKNNLVAEGNTDTSGIWKLAGISQSKIPNCSYGQYDNGLLV
                     IAEKDKDLSFVHSSWDNGIENWRFNLYGYSPEENTIAHTVLDRTLLRAGETVHMKHIL
                     RSHSTQGLSLASSNLLPTRMKISHVGSDQEYNVNLKWTGGFSEAEWKIPKQAKLGSYA
                     ISLLKGDKTYYTGEFRVEEFRVPLMKAIIKGPSEKLVKPKSFSVDLFVNYLSGGPAGG
                     MAVKLKSWFRDTAPPEVDGYEDYIFANGRLKEGTRKLSSTRNYSEDEVEDLETTSSGN
                     NAQIKTQELSLDKLGYAKTEIKDLPIKDNPQEVVTELEYRDPVGEIQTVSNVFPIYPG
                     RYLVGLKSESWSSSKDGVKIFAAVVDQNGKPKAGVDVNIDLLGRKTITNRKRLVGGFY
                     SYDSVEEVRKHITFCKGKTDAKGLLICKKESPVTGDVIFQASIEDSAGNDVFAYKEVY
                     ISGKDSWFNVSDHDRMDVIPDKKSYEPGETAKFQIRMPFKTATALISVNREGIVDYFV
                     KEIQSSSPNIEVPIKSNYAPNVFISVLAVRGRVGEIKPTALVDLGRPSYRFGISPIKV
                     GWKGYELKVNVKTDRPVFKVREKATVTIDVKTANGSKLPDNGDIALVAVDEGLLELMP
                     NNTWELLRAMMNERGLTVNTSTAQMQVVGRRHYGLKALPPGGGGGKDSSRELFDTLLL
                     WKGSVKLNSSGQAEVVVPLNDSLTRFKIVAIAMAGDKYFGTGSTSIRTTQDLMLLPGL
                     PQLAREGDTFNPSFTVRNATEKPMKVDVSGTISGVSEKLETQSIPLDAGQSKEVTWSI
                     KIPFGVEKLTYEFSAKSESGAMDKVKFAQKIVPAIPVRVQQGTLLQLENDYAMTVEKP
                     SDALEGRGGINVTYSKSLLESLSSVQDYMKKYPYTCLEQQTSKFVVTKDKQRWQNLMN
                     NLPPYLDGDGLAKYFPNSYEGNPTLTAYLLSISNEAGLEIPSESKESMLNGLTAFVEG
                     KIYRDGILPTADLTIKKLNALEALSRYGRAKNEHIATLSIQANLLPTSALLDWWNILY
                     KLKDIPKRNEHINQTEQILRSRLNLQGTTMKFTSEESDFLWWLMVSPDQNAVRLLLNL
                     VNLKKWKEDVPRIVRGAISRQRKGTWDLTTANAWGVLAFEKFSKEYEKEPVSGESSVL
                     LKEEVKSADWKANPKGLTLSFPWTKEASDLKLSHKGTGKPWATIQSTAAVSLKNAVSN
                     GYTIEKTITPLEKRGIFGIGSTLKRGDLVRVSLKIKSDSDMTWVVVNDPIPTGASILG
                     GGGLRDSASATMGQKKSGYAAYPTYEERSFESYRAYYEYLPQGEIVIEYTIRLNQDGK
                     FSLPATRVEAMYYPDMYGEFPNQLWEIEKE"
     gene            complement(8707..9189)
                     /locus_tag="PLF66_19515"
     CDS             complement(8707..9189)
                     /locus_tag="PLF66_19515"
                     /inference="COORDINATES: protein motif:HMM:NF013331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="truncated hemoglobin family protein"
                     /protein_id="HMZ66289.1"
                     /translation="MRDIDAIIEEKTGLSIEQAMRIDEVNLYKLLGEETFWKLSKTFY
                     EKVFNDEEKWFRNIFKNKSKEEAIQNQAEFFMQRMGGPQYFSERKGHPALMARHIQFN
                     MSEKAAERWLFHMKNTLDEIPEIDADSKSRMMAYFTHTAYFLSVGVTTQQRERRRDEE
                     "
     gene            9298..10242
                     /locus_tag="PLF66_19520"
     CDS             9298..10242
                     /locus_tag="PLF66_19520"
                     /inference="COORDINATES: protein motif:HMM:NF018649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HMZ66290.1"
                     /translation="MFKKFLSRTLGYLPFIGCFFILYYSPIFSNFVFWNLIIQLILFL
                     FLACIPAYFTNKMYFVDIAWPWGLVAIGIQVFIFGGNGISHYFIGSLYILAGLRMGLM
                     GVMLLRYGVLKRDLPRYQFQRFHWKRGGYKSEAISIQYEILVQCLANASILAMPAVLQ
                     AFNPKTELSILEIIGYIGWLISISLEMISDLQKNQFANKMRETGEKNKVCDVGLWKYS
                     RHPNYFFEWMVWNFLILSSIPSLIHLFKSDEILYWGLITLCLLYVSKIMYTTLVDYTG
                     ARPSEFYSIKKRPSYREYQKTTNMFFPGPRKNILKENV"
     gene            10239..12236
                     /gene="ligA"
                     /locus_tag="PLF66_19525"
     CDS             10239..12236
                     /gene="ligA"
                     /locus_tag="PLF66_19525"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008589486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HMZ66291.1"
                     /translation="MSKIEKEIRDLEDKIRYHQHLYYVKNKPEISDKEFDLLFKKLQK
                     LEAENPELASPNSPTQIVGSDLDNEFEKVTHKIPVLSLENTYNINELTEWITKTDTEG
                     EYSVEWKIDGASIVLYYEKGKLDKAVTRGTGGIGDDVTANIRTIKNIPLILKDPVTIY
                     VRGEVHMGFKDFEEFNEESGGKYANPRNLAAGSIKHKNSSQTAKRPLKILTYDGYFPE
                     GYGKIKTHAEMLKFMKNQNLPVSEDSIIVKAKDVIKKVEEFKKKKDNLDFPTDGLVIK
                     LNSLAKRDQLGATQHSPRWARAYKFDALMKESTILKIDFAVGRTGKITPRAEIEPVQL
                     AGTTVKFATLHNQDYIDELGIGIGAKVLVAKRGEIIPAVEEVIEKGPSGIFKLPSKCP
                     CCGTKLGKVDDSVDLFCPNKKCPDREKNNLIFFCQKKQMDIEGLGEKQIQNLYELGFI
                     KSIPDIYNLYKRQEELTQIDGFGKKSVDIILKGIEASKQKDFKVLLPSLGFSEVGHKV
                     TELLIESGYDDVDKIISLVKSKTAEQDLMEIHGLGPRTVESLLQVFREKETLALIETL
                     KKLGLNFKSKTVAKSDNQPFLNQSWCVTGSFQNFQPRDKAMDLIVHHGGRKVTSVSSK
                     TTHLLAGEGAGSKMEKALELGVKVVSEEEFMKLLKKEGISV"
     gene            complement(12462..14555)
                     /locus_tag="PLF66_19530"
     CDS             complement(12462..14555)
                     /locus_tag="PLF66_19530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66292.1"
                     /translation="MISKTKLFYLFVLFTILNFQCETVENRLEYFISERMKETETVQK
                     ICEDFENLNLNKKNQYIDTIGKLKEKFEKSRTDILKNSIRDLKLESGKINGVELENLG
                     SLLVLSEIDKKRFSNQEKLEWEEKYRIWQKNKKEVLEKMKEGKDISKELEILKQGMID
                     VVNLKDNLVYALKLYFTQALVVAIVHNANEAEKSLDKHTNKLGSVGIIAEAIGREIIT
                     EVMAQLVEILIPYIEKKELINPIDFTRESCKIYHKYETTPGIANRILKRSILRFLKPE
                     GEGESLVQVYSLNNINGLQNESELGTTAGLKKLEDKKREVLRKISNQNLFSNSTLECK
                     STDCKNLSENIQKDSILPAIPSENLKEKLIASYDKCLEPKEKCETETGAKNKETILTA
                     SIERCLKEKGCDWDFINQDVSWRIWHDAPSLARLEDFIFLKAKFFENSFRTNESDSSL
                     LAKIEELQGTIRILRNDIVQSMNASCAGQLEQQRNERVGWLKKLQASGFTIDTKSKVE
                     LTANNVCSLGDAIGIKKEKLVASVTASSICSFQPIEINLEIKGLFGACEFKSPDDEKL
                     ALETMNLLEILRNSLGQSSIQLQVIGFTDEVDATFADCQKKIQSGLSTKDPAIQASLK
                     DCPKPCEGNLALSNLRAFHFRNYLSSKEKLFNGQISYNAYGLSKLDAIDKSSKDLNRR
                     IKLKVFAANSKYGCY"
     gene            complement(14574..16127)
                     /locus_tag="PLF66_19535"
     CDS             complement(14574..16127)
                     /locus_tag="PLF66_19535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66293.1"
                     /translation="MKEKFLPYLIYLIAFLFILQFRYNLDKNSLYITSDGAIKLYQTA
                     QYKENGFFSLECIYPAKEIDATYKYYPISYPWAIFTVKNIPCVLEYPPFFYWIGSVLL
                     YFQSVSLLLYLPLLFFGINIFIFDLVLQRMRLIPSYRIITTLLVFISFPLLTAMDYTE
                     NPAFQTLYLLGFYYGFRLTQNSLNTVQTFLTGLFFGLAFFLRLEILIPFAFFCGFYFL
                     GTREFKKTFLLSLGFILVVSVFILYNLKVSGHPLGFRYVSSIDFNDNAKADIWKRLNF
                     LKATLWGNQMMVGVFKFQPLLWLFLILPIYAHAKGFRSKSGFILLAAGWTSMIAIPLY
                     ITVYGGVGYFGLRYIEAPFYLIALGWSFYLSELNFFQEKRIKWIIVFILVGISYFNWV
                     STREGLKLLKNSSSENAVLQTFLKQTERYVIHSSFYTSIWMGNSFLERKHVHIKGNEQ
                     MEEFLNHIARNEIFILLLSPKDIYISEDIPKKLHENYLAQIELEKFPIQILEEKKLNG
                     IRLVKARKN"
     gene            16412..18421
                     /locus_tag="PLF66_19540"
     CDS             16412..18421
                     /locus_tag="PLF66_19540"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HMZ66294.1"
                     /translation="MVGIFFQAWFIIFICFYLYFNDAQPTLVKELSANTFIGFLLFSI
                     WQKKKIKDVESFFFIFSSLCLILYTSLTGMIFLPDSLVARQKLYLRFIFYLSIAVFFV
                     LYRLDVDKKLLVFYTVLASSHIYLYRYHYSSIAILFYLVANLSLLKRFSLSKQNIFDI
                     LVFLLFTASITSTYSSFFYNGTNAVFGFFHLVSGLILYLYFKYLEDPEVISVIRYSQF
                     FYGVCLSLYFIFILIYIFSHDFNPNFSLSIGGFHVSNIGSMVAVNLPATIVIYLFYPT
                     NKKSNYIYLSLIVLSFIILYYTHSRASTLGVIVSSFFIIIYFLKFNSSRKISILPILL
                     FLSLFILVFIGKKLMHSDVFNTNSLIARKSIWEAYIDRVFHHSILFGFGSNNEFFNTF
                     LPIDYLSETVLTDLRYYFSNFQANPHAHNLYIQFLYNYGLYGLLILTGIILATFALLA
                     QKIIRNDISFNEMIAYAVLSSIFFQEIFDYTMLDAMTYFQAAFALGIISRYSHQIKSF
                     EHLKSFSTVNIKIIYALQFFICFFISMIGFNLCLSEKVKMLFKNEYTIDNFSNLKFKD
                     VSVFSDNKIKTFRKLDSMFLPINLDDKKEQFSAQIYLEVYKIKKDKKDLDLAEKNFQK
                     CILIFPNSAVCYKKLEEIELLRENIDSANIYNKKYKENDPFGLVN"
BASE COUNT         6281 a         3318 c         3138 g         5806 t
ORIGIN      
        1 gcacaaaact aatttttttt cccctatcat aaagagggat actcgaccaa atcaaagaaa
       61 gattacattg ttttacttag attctcaaag agtatgaaat tgttacttct tttgccagtc
      121 aatcctagat aaaaaagttt ccctgtatga taaacctagg atttataaaa aagtatttgg
      181 tccctttttc tctcaaatct gaaaaaagga aaatttatga atttagacca agatcctaat
      241 aatcaagaaa attcggaaag ccaaactacg tataatgaat ttctaagcga gtttcgtaaa
      301 tacctttatg aaaaattcgc aggctttcaa attgaaagtc ataagaacgc cagctctatt
      361 tcatcacaaa aaattgtatt tccacatgaa gattttaaat attctgaatt tagctcaacc
      421 ctcctcattc cagaacgcta ctatgaaatt tttcgaagag gatgctggca acatggtgga
      481 acgagaggct atgtcgccta tcttctttat aaataccaac tctttatagc aaatggttta
      541 gttccaggct attcaaaagt aaccactaag tatcaagaaa aagggcaaaa tcttcataag
      601 gttgcttttc gtccgagacc agaagactgg gcagaactta aactctatcg tgtaagtttt
      661 ggtatgtcta tctcagcttt tttggtatac ctactaatag cagactctgt agattttgca
      721 gaggctgtct cctattacct aacggcggtc ggaatttcaa gtatcccgaa tttcgacttg
      781 tgcgccaaaa tctttctaac taacaacagg tccgactata caatcttttt tcaatttagg
      841 aagagcaaat acgggtaaaa aaacaaattt ccctctacaa ccaactacca aaatcaaacc
      901 tatctatatt caaaaggcga agccatgcta acactttaag gaaactaata tcgtataacg
      961 ttttaagata tactattcaa aatataaatg gaataaagcg cttcttattt ttaaaatgtt
     1021 cagcataaaa gggattttga aacaaagctg tttcttcctg cttaattcta aaatacagaa
     1081 tcgaaaaatt acaaagacta aaaaaaacaa aggaatagaa agccccatgc agaagggaga
     1141 tggttgcgat ttccaaaatt acaatcaaat agttaggatg gcgaataaat ttatacaaac
     1201 cagtggtagc tatgctattt tttgagttta tatcatacac aactttaaca ttccaattct
     1261 cttttaaagt tgttagaaca ctaattctca ttcccaagca tcccaagtag ataaaaaggc
     1321 agaagagacc ctgtgtaatt tcaaatactc ttttaaaata aaaaatttcc aaaggaacca
     1381 aaactagaaa gctactatgt aatactacga atagaaagaa aaacggttct cttgccattt
     1441 ttaaatttgg atcagctttt ctttttgcta aatttctttt tgctaatctt agttcaaata
     1501 aacgaaagat aatcgtaact atccacaccg acaaaaaaag gaatccacca aaatttaatt
     1561 caaacatcaa ttcaccctaa ttgtgcaatc gcccttggtt gcagttaata aatattgtat
     1621 cgtgtaaaat ctcgaattta ttgaaatgac tggatttata acaatattag atccattagc
     1681 agtatactct gtaggaacac atgatggaga aaacaataat acccttgtaa aatttgcaca
     1741 acggatagta atcgtatttt gagctgtatc tgtagaatag ggatttgtaa aaattaaatc
     1801 agatcgaaat gctttagtag tgagactatc gtatgtcact gtttgactcg ttccagttcc
     1861 gacagctgtt tctgatccaa attgattatt gaaggctcca tagcaatcga tataaaaatc
     1921 atctccatcg caatttatac ctgattttct atctgcatta gaacaagcac ccccattatt
     1981 tgcgaccgtg ataggtccgg ttgatgcccc gctcgggact ttaaacgtta gttgatctgt
     2041 tcccgcataa tcaacaatag cctgaatacc atttatagat actgtattgc cagtagtgga
     2101 ggcaataaaa ttcttacctt taatgttaat agtagttgca ttgtaactag ctccaatgaa
     2161 tccaacagag cttggagttg taaatggagg atttcctctc ttggggtcga cgctaaaaat
     2221 ctgaggacta taaagacttt gaccaattaa aaggctcgcg tatggatttg tcttagctgt
     2281 atctttcttg caatgaaaga taccaattat taaaataaaa aatagaattt taactcgcat
     2341 tcctttcatt ttaatttgaa tttttaaaaa gcaagaaatt ttcccgagta gtcaaaaact
     2401 ttttctgact tgacttaatc ttaagataca attgcagtaa agagagaggg tatatatgaa
     2461 acaactttta gctttccttt ttataattcc atttttaaat ctattatctg agtcacaacc
     2521 tttaacaaat ccaaacaagg ctgattcaaa atctacaact aaaactgttg aagcaaaaac
     2581 tgtagacgcg aaatctacag aaacaaagcc agctgagccc aaagctcctg ccaaagcaga
     2641 tacaaatgtt aagatcgaaa cgcctcctaa aaaagaatta ccacctcttc aaaaggaagg
     2701 cagcttttat gtttacaaag gagttaaatg gcaagcggac aatataacac agaaagccta
     2761 tctgagtgcg aaagactcct gtgaaaaaga gggaatgcga cttccaacaa aagatgagct
     2821 attagatgca tttcaatcta aacatgttga atttaggact cctgcaggtg cctatataac
     2881 cggaaatcga gttgccagtg atcgttccaa gatttggtat ataaactttg ataatggtca
     2941 tcacaaccat ggtagctttc aaagagaatt taatgtgaga tgtgtaaaac aagagccgcc
     3001 tgcaaaaact gaggctaaaa cagaagcaaa gaccgaagta aaaactgaag ctaaaaaata
     3061 atcttaaaaa acaaggtctc tatgaaaaaa tatcttattc tatcttttct tttacttagt
     3121 aatttctttt cgttgattgc acaagagaaa aatacaatca ctagtttctc tccagaaggt
     3181 gtcgtaaaaa atataaagca agttaaagtt agcttttcta gccagatggt tcctttcgga
     3241 aaccctagag ttaaaaccga ccaattcgaa atcaattgtc caatagaagg caaggctaga
     3301 tggatagatg agaaaacttt tgtctatgag tttgcgaata acttagaagc tggaattcag
     3361 tgtaaattcc agctcaaacc agaagtaaaa acgttatccg gcaaagacat ttctggaaat
     3421 aagacattta gtttttcaac aggaggacct gcaattctat acacaaatcc ttatgagggg
     3481 aataatatag atgaggatca aatttttatt ttaaccttag atgccgatgt aaaagaaagc
     3541 agtcttgaaa ataaattggc atttgaaatt gaaggagtta aagatcgagt agccgctcag
     3601 gttataaaac catcggatgc acaagaaatt ttaaaatccg aatttggcga aagatataaa
     3661 actgataaat tacttttaat aaaatctaaa cttcgatttc caaataaagc caaagtaagt
     3721 cttatctggg gaaaaggaat tgaatctaaa tcgggagtaa agacagacga agatagagtt
     3781 ctcacctaca gtgcaagacc acctttcaca gctgaatttt cctgcgatcg cgaaaataaa
     3841 aatgcagggt gtcttcccat cacaaatcta tacgttaact tcacaagtcc catttcaaaa
     3901 tcagatgcta agaaagctac cttagaggga aatggaagaa aatggaatgc aaaaatatct
     3961 ggttcgaatg aagaagagga tggaacgaca tacggaatat actttgaagg accgttcccc
     4021 gaagaaacta aattcaaaat ttcaattcct gaatccataa aagatgactc tggacgaaaa
     4081 ctagcaaatg caaataaatt cccactcgaa gtcaaaacgg ataaataccc accactagca
     4141 aagttctctg gatcttttgg aatcattgaa ctagaagcag aaccattact gcctgtaaca
     4201 ctccgcaatt tagaaaaaga tgtgaaagca aaagttctaa aaatgaccgg cgaaggaggg
     4261 aatttctttt ccaatctttt tggtaaaaaa attaaaatca cacctgacaa tattctaatc
     4321 tggcttaaaa aagtcagtaa agcagaaaga gaaaaatcca tttttgcagg cgaagtgaat
     4381 acaaactctt ttaatattcc caaacccaat ggggaaaaag cttttgaagt tgtcggaatt
     4441 ccactcaaag aacctggatt ctttgttgta gagttagaaa gtgaaaaatt aggaaattca
     4501 ctcttagaaa aaaaagggaa aatgtatgtt ccgacatctg cgcttgttac gaatatggca
     4561 gttcactttc aatggggacg agaaagttcg cttgtctggg ttacatctct tagcaaaggc
     4621 gagcctatca gtggagcaaa aatttctata cgcgactgca aaaataattt agtcgcagaa
     4681 ggaaatactg atacgagcgg tatatggaaa ttagccggta tttctcaaag taaaattcca
     4741 aattgctctt acggtcaata tgacaatgga cttttagtca tagcagaaaa agacaaagat
     4801 ctatcctttg ttcactctag ctgggacaat ggaattgaaa attggagatt caatctatat
     4861 ggatattctc ctgaagaaaa tacaattgca cataccgttc tagatcgcac actcctgcgc
     4921 gcaggggaaa cggttcatat gaagcatatc ctccgaagtc atagcacaca aggactttct
     4981 cttgcttctt ctaatctcct tcccacacga atgaaaattt ctcatgttgg ttctgatcag
     5041 gagtataacg tcaatctaaa atggacagga ggattttcag aagcagagtg gaagattccg
     5101 aaacaagcca aattaggaag ttatgcgatc agtctcttaa aaggagataa aacctattac
     5161 acaggagagt ttagggtaga agaatttaga gtccctctca tgaaagcgat cataaaagga
     5221 ccttctgaga aattagtgaa gcctaaatct ttcagtgttg atttatttgt aaactatcta
     5281 tcaggtggac ctgctggtgg aatggcggtc aaactcaaat cttggtttag agatactgca
     5341 ccacctgaag ttgatgggta tgaagattac atttttgcaa atggaagact caaagaagga
     5401 actcgtaaat tatcctctac tcgaaattat tcagaagatg aagttgagga tctagaaaca
     5461 acttcctctg gaaataacgc acagatcaaa acacaagaat tatccctaga taaattagga
     5521 tatgcaaaaa cagaaatcaa agatttacca ataaaagaca atccacaaga agttgttact
     5581 gaattggaat atagagatcc agtcggagaa atccaaactg tttccaatgt atttcctatt
     5641 tatccaggaa gatatctagt tggattaaaa tcagaatctt ggtcttcctc taaagatgga
     5701 gtaaaaatct ttgcagcagt tgtggatcaa aacggaaagc ctaaagcagg tgtagatgta
     5761 aatattgatt tacttggtcg taaaacaatt acaaatagaa agcgactcgt aggtggattc
     5821 tattcctacg actcagtaga agaagtaaga aaacatatta ctttttgtaa aggtaaaaca
     5881 gatgcaaaag gtttactcat ctgcaaaaaa gaatcacctg tgaccggaga cgttattttc
     5941 caagcatcta ttgaagacag cgcgggtaac gatgtatttg cctacaaaga agtttatatt
     6001 tcaggaaagg attcttggtt taatgtatct gatcatgata gaatggatgt aattccagat
     6061 aaaaaatcct acgaaccagg cgagactgct aagtttcaaa ttcgaatgcc atttaaaaca
     6121 gcaactgccc ttataagcgt taaccgcgaa ggcattgtag attatttcgt taaagagatt
     6181 caatcttcta gtccaaacat tgaagttcct atcaaatcca attatgcccc caatgtattt
     6241 atctcagtct tagcggttcg cggtcgagtg ggagaaatta aaccaacagc actagtagat
     6301 ttaggcagac cttcttaccg atttggaatt agtcctatca aagtaggctg gaaaggctat
     6361 gaattaaagg taaatgtcaa aacagatcgt ccggttttta aagtgagaga aaaagcaacc
     6421 gtaacgattg atgtgaaaac agcgaacgga tccaaactac cggataacgg tgatattgct
     6481 cttgtcgcag tcgatgaagg tttacttgaa ctcatgccaa ataatacttg ggaattgctt
     6541 cgagctatga tgaatgagcg cgggctaaca gtaaatacct ccacagctca aatgcaagtt
     6601 gtcggaagaa gacactatgg tttaaaggct cttcctcccg gtggtggcgg tggtaaagat
     6661 tctagtcgtg aattatttga taccctttta ctttggaaag gcagcgtaaa attaaactcc
     6721 tctggtcaag cagaggtagt tgttccgctt aacgactctc tcactcgctt caagattgtt
     6781 gcgattgcga tggcaggaga taaatacttt gggacaggaa gcacgtccat tcgcacaacg
     6841 caagacttaa tgttactccc tggacttcct caattagcga gggaaggaga tacatttaat
     6901 ccttcattta cagttcgaaa cgcaaccgaa aaaccaatga aagtagacgt cagtggaaca
     6961 atctcaggag tctccgaaaa attggaaaca caatccattc ctctagatgc gggacaatcg
     7021 aaagaagtaa cttggtctat taaaattcct ttcggagtgg aaaaactcac ctacgaattt
     7081 tccgcaaaat cagaatccgg cgcaatggat aaagtaaaat ttgcccaaaa aattgtccca
     7141 gcgattcctg ttagagtcca acaaggaact ctcttgcaat tagaaaatga ttacgcaatg
     7201 actgtagaaa aaccgtctga cgcattagaa ggtcgtggag gtatcaatgt tacttattcc
     7261 aagagcttac tcgaaagtct aagttcagtg caagactata tgaaaaaata tccatacact
     7321 tgcttagaac aacaaacttc taaatttgta gtcactaaag acaagcaacg ttggcaaaat
     7381 ctaatgaaca atttacctcc ttacctagat ggagatggtc ttgcaaaata ctttccaaat
     7441 agctatgaag gaaatccaac gttaaccgca tacttactct ctatttcaaa tgaagctggt
     7501 cttgaaattc caagcgaatc aaaagaatct atgttgaatg gtttgacggc tttcgtagaa
     7561 gggaaaattt atcgagatgg tattcttcct accgcggatt taacgataaa aaaattaaat
     7621 gcattggaag cactttctag atatggtcga gcaaaaaatg aacatatcgc aactctttca
     7681 atccaagcaa atctacttcc cacatccgct ctactcgact ggtggaatat tttatataaa
     7741 ttaaaagaca ttcctaaaag gaatgaacat atcaatcaaa cagaacagat tctccgaagt
     7801 agactcaatc tgcaaggaac aacaatgaag tttacttctg aagaaagtga tttcttgtgg
     7861 tggctcatgg tctctcccga tcaaaatgca gtccgtcttt tattaaatct agtaaatcta
     7921 aaaaaatgga aagaggatgt gcctagaatt gtaagaggag caattagtcg tcaaagaaaa
     7981 ggaacttggg atcttacaac ggctaacgca tggggagtcc tcgcttttga aaaattctct
     8041 aaggaatatg agaaggaacc agtttctggc gaaagcagtg tcttgctcaa agaggaagtt
     8101 aagtccgctg actggaaagc aaatccaaag ggattaacac tgagtttccc ctggacaaaa
     8161 gaagcaagtg atctaaaact aagtcacaaa ggaacaggaa agccttgggc aaccattcaa
     8221 agcactgccg ccgtttcttt aaagaatgca gtctccaatg gatacacgat tgaaaaaaca
     8281 attacaccac tcgaaaagcg aggaatcttt ggaattggct ctactctaaa acgaggagat
     8341 ctagttcgag ttagtttaaa aatcaaatcc gattccgata tgacttgggt tgtagtaaat
     8401 gatcctattc cgactggcgc atctatcttg ggtggaggtg gacttagaga ctcagcaagt
     8461 gcaacaatgg gacagaaaaa atctggctac gctgcctatc ctacatacga agaaagatcc
     8521 tttgaatcct accgagcgta ttacgaatat ttgccgcaag gagaaattgt aattgaatat
     8581 acaattcgtc tcaaccaaga cggtaaattc tcattacccg caacgcgtgt agaagcaatg
     8641 tattaccctg atatgtatgg agaatttccg aatcaactct gggagataga gaaggagtag
     8701 gcttacctac tcctcatccc ttctgcgttc tctttgttgt gttgtaacac caacagagag
     8761 aaaatatgct gtgtgggtga aataggccat cattctagat ttggaatctg catcgatctc
     8821 aggaatttca tctaacgtat ttttcatgtg aaagagccac ctctcagctg ctttttcact
     8881 catattaaat tgaatatgac gagccatgag cgcaggatga ccttttctct ccgagaaata
     8941 ttgtggtcca cccatacgtt gcataaaaaa ttcagcttgg ttttgaattg cttcttcttt
     9001 acttttattt ttaaaaatat tgcgaaacca tttttcttcg tcattgaaaa ccttttcgta
     9061 gaaagtctta ctcaatttcc aaaaggtttc ttcccctagc agtttataga gatttacctc
     9121 gtcgattcgc attgcctgct caatcgaaag tcctgttttt tcttctatga ttgcatctat
     9181 atcacgcata aaattaattt tatgaatagt aagtttaggt caaatcaaaa tcttctactt
     9241 ctcacaaatt gactgttctt gacagaatcc tttctagttt taatttatga aattatcatg
     9301 tttaaaaaat tcctctcccg cactctcggt tatctaccct ttataggatg ctttttcatt
     9361 ctctattact ctcctatttt ttcaaacttt gtattttgga atttaattat tcaactgatt
     9421 ctatttttat ttcttgcctg tatccctgca tacttcacaa acaaaatgta ttttgtagat
     9481 attgcttggc cctggggatt agttgcgata ggcattcagg tctttatttt tgggggaaat
     9541 ggaatcagtc attattttat tggttccctt tatatcctgg caggactccg catggggcta
     9601 atgggtgtta tgctcctacg ctatggagtt ttaaagcggg acttgcctag atatcaattt
     9661 cagagatttc attggaagag aggtggatat aaaagcgaag ccatttcgat tcaatacgaa
     9721 attttagtgc aatgccttgc caatgcatcc attcttgcaa tgcctgctgt tttacaagca
     9781 tttaatccaa agaccgaact ttcaatttta gaaatcattg gctatatagg ttggctaatc
     9841 tctattagcc tcgaaatgat ttcagattta cagaaaaatc aatttgcaaa taaaatgaga
     9901 gaaacgggag agaaaaataa agtatgtgat gtagggctct ggaaatattc ccgtcaccct
     9961 aattatttct ttgaatggat ggtttggaac tttttaattc tttcttctat tccctccctc
    10021 attcatctat tcaaatcaga tgaaatcctc tattgggggc taattactct ttgcctactc
    10081 tatgtatcta agatcatgta tacaactctc gttgactata caggtgctcg cccttctgaa
    10141 ttctattcga tcaaaaaacg acctagttat agagaatacc aaaaaacaac gaatatgttt
    10201 tttcccggac ctagaaagaa tatacttaag gagaatgtat gagtaaaatt gaaaaagaaa
    10261 tccgtgactt agaagataaa attcgttacc atcaacatct ttattatgta aaaaataaac
    10321 ctgaaatctc tgataaagaa tttgatcttc tctttaaaaa attgcaaaaa ctagaagcgg
    10381 agaatcctga attagcctct ccgaatagtc caacacaaat tgtaggctcg gatttagaca
    10441 atgaattcga aaaagtaact cacaaaattc ctgttctctc tttggagaat acatacaata
    10501 tcaacgaact gacagaatgg attactaaaa cagatacaga gggtgagtat tcagttgaat
    10561 ggaagataga tggagcatct atcgtattgt attatgaaaa agggaagcta gacaaagcag
    10621 taacacgtgg aacgggaggt attggagatg atgtaacagc gaatatccgc acaatcaaaa
    10681 atatcccgct catcctaaaa gatccagtga cgatctatgt gcgcggtgaa gttcacatgg
    10741 gttttaaaga ctttgaagaa ttcaacgaag aaagtggagg aaaatatgca aatcctcgta
    10801 acctcgcggc aggttcaatt aaacataaaa attcctctca aactgcaaaa cgtcctttaa
    10861 agattctgac gtatgacggt tattttccgg aaggttatgg aaaaattaaa acgcatgcag
    10921 agatgctcaa gtttatgaaa aatcaaaacc ttcctgtctc ggaagattct atcatcgtaa
    10981 aagcaaaaga tgtaattaag aaagtagaag aatttaaaaa gaaaaaagac aatctagatt
    11041 ttccaacaga tgggctcgtg attaaactca acagtcttgc caagagagat caactagggg
    11101 ctactcagca ttctcctcgc tgggctcgtg cctataaatt cgatgcgctc atgaaagaga
    11161 gcacgatttt aaaaattgat tttgcggttg gtagaacagg aaagattacg ccgcgtgctg
    11221 aaattgaacc agtccagtta gcaggaacaa ctgtaaaatt tgcaaccctc cacaaccaag
    11281 actacattga cgaattagga attggaatcg gtgcaaaagt cttagtagcc aaaagaggag
    11341 aaatcatacc cgcagtagaa gaagtcattg aaaaaggtcc cagcggcata ttcaaattac
    11401 cctctaaatg tccttgttgt ggaaccaaat taggaaaggt agatgactct gtagatttat
    11461 tttgtccaaa taaaaaatgc ccagatcgag agaaaaacaa tcttattttc ttttgccaga
    11521 aaaagcaaat ggatattgaa ggtcttgggg aaaaacaaat tcagaatcta tatgaattgg
    11581 gttttataaa aagtattcca gacatctaca atctatacaa gagacaagag gaactaacgc
    11641 aaatagatgg gttcgggaaa aagagtgtag atattatact caaaggtatc gaagcctcaa
    11701 agcaaaaaga ttttaaagta ttacttccct ctcttggatt ttcagaagta ggccacaaag
    11761 ttacagaact tttaattgaa agtggatacg atgatgtaga taaaatcatt tccttagtga
    11821 aatccaaaac cgccgaacag gacttaatgg aaattcatgg actaggtccg cgcacagtgg
    11881 aatccctact ccaagtcttt cgagaaaaag aaacgttagc tctgattgaa actttaaaaa
    11941 aattaggttt aaattttaaa tccaaaacag tcgccaaatc agacaatcag ccatttctaa
    12001 atcagagctg gtgtgtcacg ggcagttttc aaaactttca accccgcgat aaagcaatgg
    12061 atttaattgt ccatcacggt ggacgtaaag ttacttctgt ttcctctaag acaactcact
    12121 tactcgcagg cgaaggcgct ggttccaaaa tggaaaaagc actcgaacta ggagtaaaag
    12181 ttgtttcaga agaagaattt atgaagcttc taaaaaaaga aggaattagt gtataacgtt
    12241 tattccaaaa tcaaagtttg aagaggaaaa ccagaatgat taccaccgac gaggacgatg
    12301 aacaccgata aatcataaag ttttcgaatt gtttttttat aaaatcttat gatctggtgt
    12361 atcttccgat tcggcttaat tcgcctgagt tgaatcaatt atattattgc gaaatttaaa
    12421 aagaatatca ttggcatcaa ttgcaatatt ctttttcttt tctaataaca accatatttt
    12481 gaattcgcgg caaatacttt tagtttgatt ctacgattta gatctttact tgatttatct
    12541 atagcgtcta acttactaag accataagca ttgtaagata tttgaccatt aaagagtttt
    12601 tctttagaag aaagataatt tctaaaatga aaagctctta ggttggacaa tgcaagattc
    12661 ccctcacaag gcttgggaca gtcttttaga cttgcctgaa tggcagggtc tttagtagaa
    12721 agccctgact ggattttctt ttgacaatct gcaaacgttg catccacctc atcagtaaaa
    12781 ccaatcactt gcaattgaat agaagattga cccaaactat ttctaagtat ttctaaaaga
    12841 ttcatcgttt cgagagcaag cttttcatcg tctggagatt tgaattcaca agctccaaag
    12901 agacctttta tttctagatt gatttcaatt ggttgaaacg aacaaataga agaggcagta
    12961 acacttgcca ctaatttttc ttttttaatt ccgattgcat cgcctagcga acaaacgttg
    13021 ttagccgtaa gttctacttt cgattttgta tcgattgtaa atccactcgc ttgtaatttt
    13081 ttaagccagc ctactctctc atttctttgt tgctccaatt gaccggcaca acttgcattc
    13141 atactttgca ctatatcatt tcttaggatt cgaatcgttc cttgcagttc ttcaatcttt
    13201 gctaaaagag aggaatccga ttcatttgtg cgaaaggaat tctcaaagaa cttagccttt
    13261 aaaaaaataa aatcctccaa gcgagcgaga gagggagcat catgccaaat tcgccaacta
    13321 acgtcctgat taataaaatc ccaatcacaa cctttttctt ttaagcatct ctctatggaa
    13381 gcagttagaa ttgtctcttt gttttttgct cctgtttctg tttcacattt ttcctttggc
    13441 tctaagcatt tatcgtaaga ggcaatgagt ttttctttta aattttcact cggaatggct
    13501 ggaagaatag aatccttttg aatgttctca gatagatttt tacaatctgt ggatttacat
    13561 tctagagtcg aattcgagaa aagattctga tttgaaatct ttcgaagcac ttctcttttt
    13621 ttatcttcta gtttttttaa cccggcagtt gtccctagtt ctgattcatt ttgcaatcca
    13681 tttatattat tcaagctata gacttgaacc agagattcac cttctccttc cggttttaaa
    13741 aatcgcaaga tagatcgttt taaaattcga tttgctatac cgggtgtcgt ttcatattta
    13801 tgatatattt tacagctctc tcttgtgaaa tcaattggat tgattaattc ttttttttct
    13861 atataaggga ttaaaatttc tacaagctgt gccattactt ctgtgattat ttcacgaccg
    13921 attgcttctg caataatccc cacactccct aatttatttg tatgcttatc taaacttttt
    13981 tctgcttcgt ttgcattgtg aacaatggct actacgagtg cttgtgtaaa ataaagtttt
    14041 agtgcatata ctagattgtc ttttagattg actacatcaa tcataccttg ctttaaaatt
    14101 tcaagttcct ttgaaatatc ttttccttct ttcatcttct ctaagacttc ttttttattt
    14161 ttttgccaaa ttctatattt ttcttcccac tctaattttt cttggtttga aaatctcttt
    14221 ttgtcaatct cagaaagaac aagaagagag cccagattct ctagctctac tccatttatt
    14281 ttaccggatt ctagttttag atcgcgaatt gaatttttta aaatatctgt tctacttttc
    14341 tcaaattttt ctttgagttt acctatcgta tcaatgtatt gattcttttt atttaggttt
    14401 aaattttcaa aatcctcaca gattttttga actgtttctg tttctttcat tctctcagaa
    14461 ataaagtatt ccaatcgatt ctcaaccgtc tcgcattgaa aatttagaat agtaaataat
    14521 acaaataaat aaaatagttt cgtttttgaa atcatatcga tttgcccttg tgcctaattc
    14581 ttccttgctt ttacaaggcg gattccgttt agtttttttt cttccagaat ctggattgga
    14641 aatttctcta attcaatttg tgctaagtaa ttttcgtgta actttttcgg gatatcttct
    14701 gaaatataaa tatcttttgg acttagaaga aggataaaaa tttcatttct tgcaatatga
    14761 tttaaaaact cttccatttg ttcatttcct tttatatgaa catgttttct ttctagaaat
    14821 gaatttccca tccaaatcga agtataaaaa gaggaatgaa tcacataacg ttctgtttgt
    14881 tttaaaaaag tttgtaagac tgcattttca gaagaagaat ttttcaataa ctttaatcct
    14941 tctcgggtag aaacccaatt aaaataactt attccaacta atatgaatac tataatccac
    15001 ttgatccgtt tttcttgaaa gaaattcaac tcagacaaat aaaaagacca ccctagagcg
    15061 attaaataaa aaggtgcttc tatgtatcta agtccaaaat agcccactcc gccatacact
    15121 gttatataga gtggaatcgc tatcatagaa gtccaacctg cggctaataa tatgaatccg
    15181 ctcttacttc taaaaccctt cgcatgagca taaataggca atattaaaaa taaccaaagc
    15241 agaggttgaa atttgaatac tccaaccatc atttgatttc cccaaagagt cgcttttaag
    15301 aaatttagtc ttttccagat atctgcttta gcattgtcat taaaatctat ggatgataca
    15361 tatcgaaaac caagaggatg cccagatact tttaaattgt ataaaataaa aacagagaca
    15421 acaagtatga agccaagaga tagaagaaaa gtcttcttaa attctcttgt ccctaaaaaa
    15481 taaaacccgc aaaaaaaggc aaagggtatt aggatttcaa gccgcaaaaa gaaagccaaa
    15541 ccaaaaaata atcctgttaa aaaagtttga actgtattta aagaattctg cgttaagcga
    15601 aatccataat agaatccaag taaatataaa gtttgaaaag ctggattttc agtataatcc
    15661 attgcagtta aaagtggaaa ggaaataaat acaagtagtg ttgtaataat acgataacta
    15721 gggattagcc gcattctttg caaaaccaaa tcaaatataa aaatattaat tccaaaaaag
    15781 agaagtggta aatacaataa tagtgagaca gattgaaaat agagaaggac ggatccaatc
    15841 caataaaaaa agggagggta ttctagaacg catggaatat ttttaacggt aaagattgcc
    15901 caaggatatg aaatcggata atatttgtat gtcgcatcaa tctctttcgc aggataaata
    15961 cactcgaggg aaaaaaaacc attttccttg tattgagctg tttgatataa ttttatagca
    16021 ccatcgcttg taatatacaa actattctta tccaagttat acctaaattg cagtatgaaa
    16081 aggaatgcaa ttaagtagat cagatatggg agaaattttt ctttcattgt tttcttagtc
    16141 ttattcctat attactgttt ttctatacaa tcctattttt ccaactcatt tcaattggca
    16201 tccaagattc tttccttttc cccgaattga atgccttttt ggttctcact cgctttttaa
    16261 aattgggaaa acagttatcg ttcaaaagaa aatagcaaac actttgaaga ataggatttt
    16321 ttatgatctg aattgaaccg atatgttttt tagaaaagaa cacttgactc aaaaggaaac
    16381 aaaacttacc ttgaattgag tccttaaaga tatggtaggt attttttttc aagcttggtt
    16441 tataattttc atttgctttt atctatattt taacgatgcc caacctactc ttgtaaaaga
    16501 attatctgcc aacactttca taggctttct gcttttctct atttggcaaa aaaaaaaaat
    16561 taaagatgta gaatcctttt tctttatatt ttcctctctt tgtttaattc tatatacttc
    16621 ccttactgga atgatatttt tgcctgactc tctagttgcc agacaaaagc tctaccttcg
    16681 ctttattttt tacctatcta ttgctgtttt ttttgttctg tatagattag atgtagataa
    16741 aaaacttttg gtcttttaca cagtcctagc ctcctctcat atttatttgt atcgttacca
    16801 ttactcttcg atcgcaatct tattctacct agtagccaat ctatctcttt tgaaacgttt
    16861 cagtttaagt aagcaaaata tttttgatat tttagtattt ttattattca ctgcctcaat
    16921 cacatcaacc tattcctctt ttttttacaa tggaacaaat gctgtctttg gcttcttcca
    16981 tttagtatca ggacttattt tatatcttta ttttaaatat ttggaggatc cggaggtaat
    17041 tagtgttata cgatacagcc agtttttcta tggagtttgc ctaagtcttt atttcatatt
    17101 tattttaatt tatattttct cgcatgattt taatccaaac ttttcactga gtattggtgg
    17161 atttcatgtg agtaatattg gaagtatggt tgcagtcaat ctcccagcaa ctattgtaat
    17221 ctatctcttc tatccaacaa ataaaaagtc caattacatt tacctctcct tgatagttct
    17281 atcatttatt atactctatt atactcattc cagagcatct accttaggag taatcgttag
    17341 tagctttttt attattatct attttctaaa atttaactca tctagaaaaa ttagcatact
    17401 acctattctt ttatttttat ctctttttat tttagtattt attggcaaaa aattaatgca
    17461 ttcagatgta tttaatacaa actctttgat tgcgcgcaaa tctatatggg aagcatatat
    17521 tgatcgagta tttcatcatt caattctttt tggatttggc tctaataatg aattttttaa
    17581 tacatttctt ccgattgatt atttatcaga aacagttctg actgatctac gttattattt
    17641 ctcaaatttt caagcaaacc cacacgcaca taatctctac attcaatttt tatacaatta
    17701 tggcttgtat ggtttactca tattaactgg aatcattctt gcgacctttg ctctacttgc
    17761 tcaaaaaata attcgaaacg atatcagttt taatgaaatg atcgcctatg cagttttatc
    17821 ctcaatattc tttcaagaaa ttttcgatta tacaatgtta gatgcaatga cttattttca
    17881 ggctgctttt gcacttggaa tcatatctcg ttattcccat caaatcaagt catttgaaca
    17941 cttgaaaagt ttttcaaccg taaacattaa aatcatctat gcacttcaat ttttcatttg
    18001 tttttttatt tccatgattg gatttaatct ctgcctttct gaaaaagtta aaatgctttt
    18061 taaaaatgaa tatacaatcg ataatttctc aaatttaaaa tttaaagatg tatccgtttt
    18121 tagtgacaat aaaataaaaa cctttcgtaa attggacagc atgttcttac caattaactt
    18181 agatgataaa aaagaacaat tttctgctca aatctactta gaagtctata aaattaaaaa
    18241 agacaaaaaa gacctagacc tagctgagaa aaatttccaa aagtgcattt tgatttttcc
    18301 aaattcggcg gtttgttata agaaactcga agaaattgaa ctacttagag aaaatataga
    18361 ctctgcaaat atttataata aaaaatacaa agaaaatgat ccctttggac ttgtaaacta
    18421 acgtattatg gagaatttta tgaatcgatt tataatagtt atgggtttaa tttttgtttt
    18481 caattgtgct ggaactagac ctacgaatct aggagtaaac aatggtaaac tccttccctg
    18541 tcc
//
LOCUS       DAOQOS010000110        18341 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_1524_length_18341_cov_5.650498, whole genome shotgun sequence.
ACCESSION   DAOQOS010000110 DAOQOS010000000
VERSION     DAOQOS010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 18341)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18341)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18341
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1524_length_18341_cov_5.650498"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(142..432)
                     /locus_tag="PLF66_19545"
     CDS             complement(142..432)
                     /locus_tag="PLF66_19545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66295.1"
                     /translation="MQFLTVRDLRNNSAQILNHLDPTEDLVLTSNGKPIAILSSVTEK
                     TLEPTWKALKQAKALAALGRIQNTSLLNETSEISESEIRKEIKAVRKSRKKN"
     gene            541..1656
                     /locus_tag="PLF66_19550"
     CDS             541..1656
                     /locus_tag="PLF66_19550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020777020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HMZ66296.1"
                     /translation="MAENSSTEAQTQAQVQTEMKQVESIENLPKEDQQKIIDLAKQIN
                     IEDTQSVLQYGSQSQKKISEFSDQILNQIRAKDAGETGKILTDLMLKVKEMDVESLSD
                     EDSFISKIPLIGSLVKSSQKFIAQYDTLSEQIEKIIDELHKSRMILLKDITLFDTMYA
                     KNLDYFKELNQYILAGEMKLAELKEKELPKYKEKAEKSNDPVDAQKYQDYMQLLNRFE
                     KKLHDLKLTKMLSLQTGPQIRLIQNSNQVLVEKIQSSIINTIPLWKNQLVIAIGLLRQ
                     KKALGVQKEVTSVTNELLSKNSEMLKQGTIEVAKEAEKGILEIETLKKINTDLIFTLD
                     ETLKIQTEGRQKRQAVEVELAKMEEEIKDKLKNTKQF"
     gene            1660..2574
                     /locus_tag="PLF66_19555"
     CDS             1660..2574
                     /locus_tag="PLF66_19555"
                     /inference="COORDINATES: protein motif:HMM:NF021617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HMZ66297.1"
                     /translation="MNESKITDEEAKWGRRSHLSLLLAYPLLIILLSKGVSFAFPIAM
                     IATMLVPLYILFTKGNQSRFIATHAKEAAFLQGFMAILGLGIHLLWGSESSTVNRLMQ
                     IGSYTASGLYHFGAVIAGSIKSSYKTLIRYPLSFFSTPNIEKEKDDFEQKMAELRALE
                     NLDKVTASMLKETMAIGKDRIAEIDRLLEKIKDPSVREKVLEIREYVVKIFENFKNDP
                     EDIKISRQFLSYYLDTTIKIIRKYIDLSSQKVISVELKESLQRVDNILSQIIEAFAKH
                     HDKLIQNDIMDLDAEITVMEKTMKMEGM"
     gene            2578..4095
                     /locus_tag="PLF66_19560"
     CDS             2578..4095
                     /locus_tag="PLF66_19560"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HMZ66298.1"
                     /translation="MDLVLIKAQTKEILQLIVPLADYYRANYKDITDFEKRVIFAEHL
                     LASEMIRIASYLYANDSTESEKTLPAIVEILKEIDKSTLYNKTKLLSEIELYVKNQPQ
                     GKKDLVIEELDPPMLLRVVKFYDSVFLTNNMYIVANYLFQILNLIVKQDGVVTEVEKR
                     LLTRYQTILHESNEDIYADSKSSSKDYLNLNSIFNDFFGSVSTTIQKDSKDKKENKEA
                     AKEDKPQGNIQEAGKEEVYDLDTLMKELNELIGLSSVKSEVSNLVNVLKVEKLRKEKN
                     LPVPVRSLHMVFTGNPGTGKTTIGRLIAKIFKALGVLEKGHLIETDRSGLVAGYVGQT
                     ANKTIEICQKALGGVLFIDEAYALAEGGENDFGREAINTLLKFMEDNRANFIVIVAGY
                     TDNMQDFINKNPGLQSRFNKYILFPDYSPEELFSIFSSLTNKSKLKLTEDASVKVKEI
                     LQSHYDKRDKKFGNGRFARNLFEKIYGNQANRLVTLTDLNEEGLSTIVLEDITDL"
     gene            4190..4450
                     /locus_tag="PLF66_19565"
     CDS             4190..4450
                     /locus_tag="PLF66_19565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66299.1"
                     /translation="MGTNRFCYFHSFTIISYGPSSDDAEKYLHLVDDGQIIYHLTIGF
                     TVVKIEGLLPIKSIEYKNSIFETRFDKIQWFKKNNIPVPDWI"
     gene            4688..5644
                     /gene="rfaD"
                     /locus_tag="PLF66_19570"
     CDS             4688..5644
                     /gene="rfaD"
                     /locus_tag="PLF66_19570"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011671724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HMZ66300.1"
                     /translation="MKKKFIVTGGAGLIGSNIVRELNQMDETDILIVDHLGNSEKWKN
                     LLDLKYKDYLEKEDFLSRIQNGNPFKKYTHAIHMGACSSTTETNASYLIQNNFEYTKI
                     FADYCLSNETEFIYASSAATYGDGANGYSDSSELSTLRPLNMYGYSKHMFDLYAKQKG
                     ISDKITGLKYFNVFGFGEAHKGDMRSVVLKGYEQIKSMGVLNLFKSYRPDYKDGEQKR
                     DFLYVKDAARITLYLLLERKYGLYNVGRGVAETWNDLGKALFKALNLPTNIHYTDMPE
                     SLRPKYQYYTKADTEKLLKTGYSDGFTSLEEAVEDYVNLLQA"
     gene            complement(5641..6441)
                     /locus_tag="PLF66_19575"
     CDS             complement(5641..6441)
                     /locus_tag="PLF66_19575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010571854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HMZ66301.1"
                     /translation="MLSAPEITRYSRNILLSEVGRKGQEKLKASVVTVVGAGGLGSPV
                     LFYLAAAGIGEIRIIDSDKVDLTNLQRQILYGTESINKSKAEEASQVITNLNSNIKIV
                     TYNERLNASNIEKIFAGSSLVIEGSDNFETKFLVNDACYFYKIPLMIAGILRFEGQVI
                     GVKPEETACYRCIFHSPPDADSVPNCAEAGVIGSVAGLVGSIQATESIKFLLGVEEGL
                     FGSILSIDAKSMEFRKIPVHKNKNCPLCGTSPSITEIKDIVLPQCNWD"
     gene            complement(6445..6903)
                     /locus_tag="PLF66_19580"
     CDS             complement(6445..6903)
                     /locus_tag="PLF66_19580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HMZ66302.1"
                     /translation="MIRRISLLVAVSCISISAEVPIDIHIKNLSSGTESDKIAACNYL
                     GKEKETKYISEVQEALKTTDSPKVAVSCANALGYFQEKGSPTTTLKNKIISVSNSDVV
                     YACLVSILNIALKNGYEADAKAAIQYSDINHRQDEFVADIIDRIKKRFKE"
     gene            7088..8428
                     /locus_tag="PLF66_19585"
     CDS             7088..8428
                     /locus_tag="PLF66_19585"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HMZ66303.1"
                     /translation="MKQFIAYYILIKSKLRLLKPTQLLTLGFLSYVFLGTFFLSLPFS
                     QKTEIKFIDNLFNVVSAVSTTGLTTISVSDSYSRLGEFILMILFQLGGIGYMTISSFI
                     IIASGKRLSHTREQILTSEYTLPKGFTIRDFVFKIFIFTVIIETMGSFMLYLEFRSAG
                     VNNPLWSSIFHCISAFATAGFSLNNNSLENFKTSPLVNFTISILCYLGALGFIVIADF
                     WFAVRSKDYKITFTSKIILWVTFVILFIEAPLFLLIEPSIASYTLWEKIQISLFQIMT
                     SSTTAGFNTVSIGGLHSASLTLILFAMIIGASPSGTGGGIKTTTFTALLAVTISMLKG
                     QTYISFFKNRVPLVRLFTAVSSVTIYLFVLTLGLFALTLTDQQDFLKLVFESASALGT
                     VGLSMGITGDLSNLGKLILTLLMFLGRVGPLTIGLAILYNPLEHDRIKDADLAV"
     gene            8525..8719
                     /locus_tag="PLF66_19590"
     CDS             8525..8719
                     /locus_tag="PLF66_19590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66304.1"
                     /translation="MKHIFYTILTIFLFIQLTSCSSQEVKEEQPDDILEEELRAEAEP
                     IREIRERDLKEEVKRPMRKK"
     gene            8730..10589
                     /locus_tag="PLF66_19595"
     CDS             8730..10589
                     /locus_tag="PLF66_19595"
                     /inference="COORDINATES: protein motif:HMM:NF024760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HMZ66305.1"
                     /translation="MLTFEKIFLSLLPIPFSILLYQRYFFRGKRYGIYLDAFLYGVLQ
                     AASLLFLFPFLDEFVNIDAKIFTGFFSAAFIEKTTAFLFLYYLLKKQYSRLNVNESVC
                     FGMFLGIGFAALENVFYAIQVTSSTIFLRTFTSVLLHITTCGMIAYYLALKQAYNTSL
                     NKSIYTTYAYILPLIFHGLYDSCLLIGNEITYIIGPELVFLILALEYMMARALSLPNT
                     EELHEKKISLEDWESIQLQPQYERWIIRSMGKKNAEFVPFFSITIRPEKGSIIVLLTL
                     ISIVFYFFQTQMVLFFDLKLSEYELTTLFAIYPLVLAFNLFLLGAINPEYFKNSIISI
                     PIIMEVNVIGGLDSKSMVGSEITAYNSFLRTFDTLPIGHELEISYLYSDFKSPPIKGK
                     IIWDNHEDFEKPMGSIIRFEETPKGFIRFLVQYNLYKLGKGILYNLKFPGFKKIRKLF
                     VKESTVMEHNNYYTAGTILFKEGESGKKFYLLKKGSIEICKHTSAGEKITLSFVESGE
                     IFGEMAILGNQPRSASAVCMTNCVVATADGDNLEALIKGNPDFSLKLIQTLATRISYS
                     EKLLKSRIQELEDALNHEKEKNHKLETKVSEMIPKPVDLKISQHSKFRKRRKT"
     gene            10666..12195
                     /locus_tag="PLF66_19600"
     CDS             10666..12195
                     /locus_tag="PLF66_19600"
                     /inference="COORDINATES: protein motif:HMM:TIGR04456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LruC domain-containing protein"
                     /protein_id="HMZ66306.1"
                     /translation="MKRKIPISLLILFFALISCDEKKKQYIPIQSVVQQNVPNTSTNG
                     SSGPTVSLPSAGGSNTDLSGVTPGTTLTTTPAGNTPFTYSNNTTIPVDSTVTNSEGTP
                     VAGAIVTITNPEANDVLLQQVTNSDGKVTGSITVPSTTTSVSLTVTSGGTTCQPVQIP
                     LQVVTTQNGNTTISQVSSIGNIAIPIANVGGNQTVADRDKDGINDDLDFYPDDSKKAT
                     KIRFPSSSVNTIAFEDLYPNAGDADLNDYVVQFFTEEDLNAEGKIVEIRGAFQHVARG
                     AGYRHQLKILLPNNLDLSYESTVTDASGVAQNTSIAKFTPTSSQLEAGLIILGNSGTT
                     ISSPNSESSQTYAPGHISKFKIVFNTPVARNVIGSSPYNVYLQVIDTGKNIFFPGKYF
                     DANGKDLYMDSNGFPWAIHVPGVWQWPLERKDIRNIDNTGYPRFNSWATSNGLNDKDW
                     YTTITNGKVYSIPTTPSNLLAYLKGVDSNISVYLAMSLAFLGIIFGLLIRKKLSNVSS
                     T"
     gene            complement(12346..12531)
                     /locus_tag="PLF66_19605"
     CDS             complement(12346..12531)
                     /locus_tag="PLF66_19605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HMZ66307.1"
                     /translation="MLVNVGSLDRIIRVVLGLGIIAAGLYFSSWFGLIGVVLLATAAI
                     KFCPLYWTVKVSTVEKK"
     gene            complement(12548..12916)
                     /locus_tag="PLF66_19610"
     CDS             complement(12548..12916)
                     /locus_tag="PLF66_19610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002978433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HMZ66308.1"
                     /translation="MNFINLMYLAVGVFGVLLALRFLNKGVGAVDKKDVIDYIKGGAL
                     IVDVRSGEEYASGHYANAKNIPVDQVESRLSEFGDKERKIVVYCRSGGRSGRAKGILD
                     ANGFKNVVNGGGLSDMPEVK"
     gene            13077..13340
                     /locus_tag="PLF66_19615"
     CDS             13077..13340
                     /locus_tag="PLF66_19615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008590863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HMZ66309.1"
                     /translation="MTKQRTDLISRLSRIQGQLESIKKSLESETDIECIKTMHLIKAA
                     NNALKKFGEAYVSNHMDECMSGKTPKKNIEKELKSVIEAAFTL"
     gene            13394..15043
                     /locus_tag="PLF66_19620"
     CDS             13394..15043
                     /locus_tag="PLF66_19620"
                     /inference="COORDINATES: protein motif:HMM:NF012861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HMZ66310.1"
                     /translation="MTGSFVILFSKLLEYFERLLSFNHSSFYKTFSFEFNFLYLLLPA
                     IGGLLVGLITHYLCSEARGNGIDHLLDAFHNQNGEMRGRVSYYKAIVTIITLSTGGSA
                     GKEGPSAQIGASIGSKINNVFAGGARARRTLLLAGAAASLGTIFQAPLGGAITAIEMV
                     YREDIESDALIPSIISSVSAYFLLSSFGLVHREYFALQELAPKYLTIYPILGLISFGI
                     GFVLVKIIQRLKAFFLKWKIFPPFKPMIGGLICGVIYLFFPVVSGTGESYIENFLFKP
                     ETYTGIPKLELIQFVLFVVTFKIFATTITIASGGSGGIFGPSLFLGGTIGMFVAEVAG
                     LFYTLDPDHYRSFILVGMGSFYAGIASAPIAAMIMICEMVGSYVLLPPLMVCSIIVFL
                     LSKKLNLYSGQVQDRFHSPAHHWDIQTDILRKMPIYHYQTYLHRSAIAKETQPIQFIK
                     RRALKRDEKDFIILNESSEYVGIISSLEFMRQDINPKSPVYPYIEKIPAISISQSLGD
                     ALELLLKHNLDKVAVISNSSRFLGYLRYQDILELYFSKLKR"
     gene            15315..17117
                     /locus_tag="PLF66_19625"
     CDS             15315..17117
                     /locus_tag="PLF66_19625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66311.1"
                     /translation="MKSNSFILKIFLLLGFILLYLQINCKSKDCNSAQTIIDKVPFIE
                     KAIDCQKEDNSLLLGLIAIGSRSSTASISTAATCPVSGSLSNPSPVNGSSITLTGAQC
                     TNSAIQSTITNGGNITLNCSGTISISSELTVPSNGTVFITGVNGSGTNGKVILDGGGI
                     SRIIYGNSSSKIIVKNLQFQNGKSKETADSTGALNKNAGGALFNGYKGEMTVIDCDFL
                     NNVGGTNASGNKEEGGGAIYTKSFGKLTIVNSYFSGNTGGLGGAINNLLSKLTIIDSN
                     FTSNSSLVDNYSAGGYGGAIYTDGAGLGGVNGANNQASLNPPSGLGNITICRTVFSNN
                     TGKGQGGGTFLYGYAPDVITLDRIVYDKNSVIKNGAGDALGGGLRSGNADTYISNSSF
                     TDNVALSQGGGFWKGESSLLSISNSTFVNNYAVETGSTTAASLSNRNNTGGICGAINN
                     AGSSSFDMTNLTIVNNHAGFMSGAICGSSNSASKSIIAYNTAYNNGNSWKKGQNCAGN
                     QIVNKGSNFEFPNKNTTDSTSDNITCFNSTIQDPLYQSSSNLTYTNSVVSPIPLATVN
                     DCSTQVGSSGRTIAIKNIALQSGSPATGSGASCP"
     gene            complement(17166..17798)
                     /gene="lipB"
                     /locus_tag="PLF66_19630"
     CDS             complement(17166..17798)
                     /gene="lipB"
                     /locus_tag="PLF66_19630"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010515426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016979 - lipoate-protein ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HMZ66312.1"
                     /translation="MKFTNKLNSVPYNRYIQLQSLLRKKRREIIIFLEHTSTITAGIN
                     YNIQNLLVSEEYLTKKEIEFCRIERGGDLTAHETGQLVIYPHIDLKKRSKNIGEYLSI
                     LTNSMIESVYSVWQLKLVSDAMRPGLYLEKDPIKKIVSMGVYFKSFFTSYGVALNLRN
                     DLNVFQLIHPCGQDAANMVSLKKLGFDISEEKELEFIKTFQKLFLEKINL"
     gene            17873..18334
                     /locus_tag="PLF66_19635"
     CDS             17873..18334
                     /locus_tag="PLF66_19635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004787699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4065 domain-containing protein"
                     /protein_id="HMZ66313.1"
                     /translation="MSKLLYYSDGVYFQRHKEMITKERYIRLEDGPELINLNLAIIHL
                     LGNGILSVEPDITAISDRIDRFFLRSISTMNLNLEKEEIRILNKVLKAFKEGVKNEDK
                     QYPNLYENYVITPIFSEIIFSEKTINTKIHVFKKKSLLNLSGKIFRVLFES"
BASE COUNT         6253 a         3187 c         3071 g         5830 t
ORIGIN      
        1 agacttctcg gtattcggca aggatcctcg catcaaaaca gagagttaaa ttgcctgagc
       61 caattctacg aacaatttct cctgggatgc taaatggttt tagaatcccg cttactagaa
      121 catttgtatc taaaacaatt ctcaattttt ctttctagat tttcggactg ctttgatttc
      181 ttttctgatt tcggactcgg agatttcaga agtttcattt aaaagcgaag tattttgaat
      241 gcgacctagt gcagctaacg cttttgcttg tttcaatgcc ttccatgtag gttctagagt
      301 tttttctgtg acggaggaaa gtatagcaat tggtttacca ttggatgtaa gaaccaaatc
      361 ctcagtagga tccaaatggt ttaaaatttg agcagaatta tttcgtaaat ccctaactgt
      421 taaaaattgc atatttaagc acctgtagca caattgtagc aatatagaac tctttgacaa
      481 gaaaaaaaat ccttgcctat ttgatttaga agtgtaagtt cgtatcagaa ggagtaacca
      541 atggcagaga attcttccac agaagcacaa acacaagcac aggtccaaac agaaatgaaa
      601 caggtagaga gcattgaaaa tctaccgaaa gaagaccaac aaaaaatcat cgatctagca
      661 aaacaaatca atattgaaga cactcagtct gttctacagt atggatctca atctcaaaaa
      721 aagatttctg aattttctga tcaaattcta aaccagattc gagcaaagga tgcaggggaa
      781 acaggaaaaa ttcttacaga tctaatgctg aaagtcaaag agatggatgt agaaagtctt
      841 tctgacgaag atagttttat ttctaaaatt ccactcattg gttctttagt aaaatcatca
      901 caaaagttta tagctcaata tgatacgtta agcgaacaaa tagagaaaat tatagatgaa
      961 cttcataagt caagaatgat tctactcaaa gacatcactc tctttgatac aatgtatgca
     1021 aagaatttgg attatttcaa agaactcaat caatacatcc tcgccggcga aatgaaatta
     1081 gccgaactaa aggaaaaaga acttcctaaa tacaaagaga aagcagagaa gtctaacgat
     1141 ccagttgatg cgcaaaaata ccaagactac atgcaactcc taaatcgttt tgagaagaaa
     1201 ctacacgatt taaaactcac taaaatgctg tcactccaaa caggacctca gattcgcctg
     1261 atccaaaata gcaatcaggt tcttgtggaa aaaatccaaa gctctatcat aaatacaatt
     1321 cctctttgga aaaatcaact tgtaattgca atcggacttc tcagacaaaa aaaagcactt
     1381 ggagtgcaaa aagaagttac ctctgttaca aatgaactac tttctaagaa ctctgagatg
     1441 ctaaaacagg gaacgatcga agtagcaaaa gaagcagaaa aaggaatttt agaaatagag
     1501 actttgaaaa aaatcaatac agatcttatc ttcactctag atgaaactct taagattcag
     1561 acagagggaa gacagaaacg tcaggcggtt gaagttgaac tcgctaagat ggaagaagaa
     1621 atcaaagaca aactcaaaaa cacaaaacaa ttttaatcca tgaacgaaag taaaattacg
     1681 gatgaagaag caaaatgggg tcgtagatcc catctttctc ttcttttagc ctaccctctt
     1741 ctcatcattc ttctctccaa aggtgtatcc tttgcgtttc caattgcaat gattgcgaca
     1801 atgttagttc ccttgtatat acttttcaca aaagggaatc agtctagatt cattgcaact
     1861 catgcaaaag aagctgcttt tttgcaaggc tttatggcaa ttcttggact aggaattcac
     1921 ctactctggg gatcggaaag ctcaaccgtt aaccgcttga tgcaaattgg tagttatact
     1981 gcatcgggtc tttatcattt cggagcagtg attgctggat ccattaaatc ctcttacaaa
     2041 actctcatac gttatccgct tagctttttt tcaactccga atattgaaaa agagaaagat
     2101 gattttgagc aaaagatggc agaacttaga gctcttgaga acttagataa agttacagca
     2161 agtatgctaa aagaaaccat ggcaattgga aaagatcgca tcgctgaaat cgatagacta
     2221 ctagaaaaaa taaaagatcc atcggttcga gaaaaagttt tagagattag agaatatgta
     2281 gtcaagattt ttgaaaattt caaaaacgat ccagaagata taaaaatctc gagacaattt
     2341 ttatcttact atctagatac aacaataaaa atcattcgta aatacatcga tctctcttct
     2401 caaaaagtaa tatcagtgga actgaaagaa tctttgcaaa gagtagacaa tattctatct
     2461 caaatcatag aagcattcgc aaaacaccat gataaactga ttcaaaatga tatcatggat
     2521 ttagatgcag aaatcacagt catggaaaaa accatgaaaa tggaaggaat gtaatccttg
     2581 gatttagtct taatcaaagc tcaaacaaaa gaaattttac aactcattgt gcctctcgca
     2641 gattactaca gagccaatta taaagatata actgattttg aaaaaagagt tattttcgca
     2701 gaacatctat tagcctctga gatgattcga attgctagct atttatatgc aaatgattcg
     2761 actgaatcag aaaagactct gcctgctatc gtagaaattt taaaagaaat tgataaatct
     2821 actctctata acaaaacaaa gcttctttct gaaattgaac tctacgtaaa aaaccaacca
     2881 caggggaaaa aggatttagt tatagaagag ctcgatccgc ctatgctact tcgtgttgta
     2941 aaattctatg attctgtatt tcttacgaac aatatgtata ttgttgcaaa ctatctcttt
     3001 cagattttaa atctgattgt aaaacaagat ggagttgtaa cagaagtaga gaaaagactt
     3061 ttaactcgtt atcaaaccat tttacatgaa tccaatgaag atatttatgc ggatagcaag
     3121 tcttctagca aagattatct aaatctaaac tctatcttca atgatttctt tggaagtgtt
     3181 tctactacaa ttcaaaaaga ttccaaagac aaaaaggaaa ataaagaagc ggctaaagaa
     3241 gataaaccgc aaggaaatat ccaagaagcg ggaaaagaag aagtatatga cctagacact
     3301 ctcatgaaag agttaaatga actgattggt cttagctctg tgaaaagtga agtctcaaat
     3361 cttgtcaatg ttctaaaagt agaaaaacta agaaaagaaa aaaatcttcc tgttcctgta
     3421 agaagtctac atatggtttt tactggaaat ccgggcactg gtaaaacaac aatcggacga
     3481 ctcatagcaa agatttttaa agcgttaggc gttttagaaa aaggtcattt gattgaaacg
     3541 gataggtcag gacttgtagc cggatatgta ggacagacag ctaacaaaac cattgaaatc
     3601 tgtcaaaagg ctcttggtgg agtattgttt atagacgaag cctatgccct cgcagaaggc
     3661 ggagaaaatg attttggacg agaagcaatt aacactcttt taaaattcat ggaagacaat
     3721 cgagctaatt ttattgtcat cgttgccggt tatacggata acatgcaaga cttcatcaat
     3781 aaaaatccag ggcttcaatc tagatttaat aaatacattc tatttccaga ttatagccct
     3841 gaagaacttt tttctatatt ctcttctctt acaaataaat ctaaactcaa actgacagaa
     3901 gatgcttcag tgaaagtaaa agaaattcta caatctcatt acgataaaag agataagaaa
     3961 tttgggaatg gaagatttgc tcgaaacctt tttgaaaaaa tttatggaaa ccaagccaat
     4021 cgcttagtca cactcaccga tttaaatgaa gaaggtttat ctactatcgt tttagaagat
     4081 ataacagatt tgtagtttta ttttaacgtc agttcgatgt aacagataat ttaaaggatt
     4141 accacctatt gccgcgaacg agcggcaatg gattttgggt ggaaagacga tgggcaccaa
     4201 taggttttgt tattttcatt cttttacaat tatatcttat ggtccaagta gtgacgatgc
     4261 agaaaaatac ttgcaccttg tggatgatgg acaaatcatt tatcatctaa ctattggatt
     4321 taccgtcgtt aaaatagaag gtctacttcc aattaaatct attgaatata aaaattctat
     4381 ctttgaaaca agatttgata aaatccaatg gtttaaaaaa aacaacatac ctgttcccga
     4441 ctggatttaa aaatttctat tacaaaaatt taataatttc aaaagtaatg gtaattttaa
     4501 aaatgcaaga aggtgttttt taaataataa aatcaacagc ctacgacagc aagccctccg
     4561 ctaagagcaa acctcatttg aacgtttggt tcacccaaac tcaatcattg cttttattaa
     4621 gcaatgataa tgtaaggtga gttaaaatta gaatttacac ctatctagaa tttcttccaa
     4681 tggaagtatg aaaaagaaat ttatagttac tggtggtgct ggtttaattg gttctaatat
     4741 cgtaagagaa ttaaatcaaa tggatgaaac agatatttta atcgtggatc atcttggaaa
     4801 ttcagaaaag tggaaaaatt tactcgattt aaaatacaaa gattatctag aaaaagaaga
     4861 ctttctatct cgcattcaaa atgggaatcc atttaaaaaa tatacacatg caattcacat
     4921 gggtgcttgt tcaagcacta cagaaactaa cgcatcttat ttaattcaaa ataattttga
     4981 atataccaaa atctttgctg attattgtct atcaaatgaa acagaattta tctatgcatc
     5041 aagtgctgca acctatgggg atggagcaaa tggctacagt gattcttcag aattatccac
     5101 tctacgtcct ctcaatatgt atggttactc taagcatatg tttgatctat acgcaaaaca
     5161 aaaaggaatc tcagataaaa tcactgggtt aaaatatttc aatgtatttg gatttggcga
     5221 agcgcacaag ggtgacatga gaagtgtagt tctaaaaggc tacgaacaaa tcaagtcgat
     5281 gggtgttttg aatttattca aatcctatcg tcccgattat aaagatggag aacaaaaaag
     5341 agattttctc tacgtaaaag atgcagcgcg tataacgctt tatctattac tcgagagaaa
     5401 atatggtctc tacaatgtgg ggcgtggagt ggcagaaaca tggaacgact taggtaaagc
     5461 tttgtttaaa gcactcaatc ttcctacgaa tattcattat actgatatgc ctgaaagtct
     5521 aagaccaaag tatcagtatt acaccaaagc agacacagaa aaacttttaa aaactgggta
     5581 ttcagatgga ttcacaagtc ttgaagaagc agtagaggac tatgtgaatt tacttcaagc
     5641 ttaatcccaa ttacactgcg gaagaacaat gtcctttatc tctgtgatac taggacttgt
     5701 tccgcataac ggacaatttt tatttttatg aaccggaatc tttctaaact ccattgattt
     5761 tgcatcaatt gaaaggatac tcccgaatag cccctcttct actccgagca gaaactttat
     5821 agattcagtt gcttgtattg aacctacgag accagctaca cttccaatca ctcctgcttc
     5881 tgcgcaattt ggcacactat ccgcatccgg tggggaatgg aaaatacacc gatagcaagc
     5941 agtttcttct ggtttaacac caattacctg tccttcgaaa cgaagaattc ctgcgatcat
     6001 cagaggaatt ttataaaaat aacaagcatc atttactaga aattttgttt caaaattatc
     6061 ggaaccttca atgactagac tggatccagc aaaaattttt tcaatattgg aagcattcaa
     6121 tctttcattg taggtaacta tttttatatt agagtttaaa tttgtgataa cttgacttgc
     6181 ctcttctgct tttgatttat tgatagactc tgttccatac aaaatttgtc tttgtaaatt
     6241 ggtaagatcc actttatctg aatctataat tcgaatttct ccaattccag cggcagccaa
     6301 ataaaacaag actggactcc ctagaccacc tgcgccaacg actgtgacaa cagaggcttt
     6361 tagtttttct tgtcctttcc gtcctacttc ggagagtaga atatttctag aataacgtgt
     6421 gatttctgga gcggataaca tagattattc tttaaatctt ttcttgattc tgtcaatgat
     6481 gtctgctacg aattcgtcct gtctatgatt tatatctgaa tattgaattg cagcttttgc
     6541 gtctgcctca tatccatttt ttagggcgat atttaaaata gaaacaagac aagcatatac
     6601 tacatctgaa ttggaaacag atatgatctt attcttaagc gttgtagtag gacttccctt
     6661 ctcttggaaa taccccaagg catttgcaca ggatacagca accttaggac tatcagtcgt
     6721 cttcaaagcc tcttgcactt ctgaaatata ttttgtttct ttttctttgc ctaaataatt
     6781 acaagctgca attttgtctg attcggttcc agaggataaa ttctttatat gtatatctat
     6841 gggaacctcc gcactaatag aaatacaaga tacagcgact aacaaggata ttcttctaat
     6901 cataactact cccaattttt tatgttaaat ttattttata aaaaatgaat gaaactacaa
     6961 tcattttata aataggaata aaagtaatcc atttttcaaa caccataaag tcttataata
     7021 ttcctccatt tttctttttt tctttcctag atatttttaa atttttccat ggatgtatca
     7081 gataaacatg aaacaattta tagcttatta tattcttata aaatcaaaac taagactctt
     7141 aaaaccaaca caattattaa ctcttggttt tttatcctat gtttttcttg gaaccttttt
     7201 tttgtcactt cccttttccc aaaagacaga gataaaattt atagataatt tatttaatgt
     7261 agtttcagcc gtatccacaa caggccttac tactataagt gtaagcgact cctactcccg
     7321 ccttggagaa tttatactta tgattctatt tcaactcggt ggaatcggtt atatgactat
     7381 atcctctttt atcattatcg caagtgggaa gcgtttatcg catacaagag aacaaatttt
     7441 aacttctgaa tatactttac caaaaggatt tacaattcga gacttcgttt ttaaaatctt
     7501 tattttcaca gtaattattg aaacaatggg aagttttatg ctctacttag agttccgatc
     7561 agcaggtgta aacaatcctc tttggtcatc catttttcat tgtatttctg cgtttgcaac
     7621 tgccggtttc agtttaaata ataatagttt agaaaatttt aaaacaagtc cattagtaaa
     7681 ttttacaatc agtattcttt gctatttagg tgcccttgga ttcattgtaa tcgcagactt
     7741 ttggtttgca gtcagaagta aagattataa aattactttc acttcaaaaa ttattctgtg
     7801 ggtaacattt gttatcctct tcattgaagc tccattattt cttttaatag aaccatctat
     7861 cgcatcctat acactttggg aaaaaataca aatatctctt tttcaaatta tgacttcatc
     7921 cacaacagca ggatttaaca ctgtatctat tggaggactc cattctgcgt ctctaacact
     7981 catcctattt gcaatgatta ttggagcatc cccaagtgga acaggtggtg gaataaaaac
     8041 gacaacattc acagcactac ttgccgtcac aataagtatg ttaaaaggtc agacttatat
     8101 ttcatttttt aaaaatagag tgccattggt tcgcctattt acagccgtgt cttcagtaac
     8161 gatttattta tttgttctta ccttaggtct atttgcttta actcttactg atcaacaaga
     8221 ttttttaaaa ctcgtatttg aatctgcatc tgcattgggg actgtgggac ttagcatggg
     8281 aatcacagga gatttatcta acttaggaaa actcattctg acattattaa tgtttttagg
     8341 acgtgttggt cctttaacaa ttgggctcgc gatcctatac aacccactgg aacatgatag
     8401 aatcaaagat gctgatctag ctgtttagaa aaagcataaa tccagtaaga aattgttgtc
     8461 aaaaaatttc acttgcatat ttttacacat tttattagtc ttattgcata tttagagagt
     8521 ctttatgaaa catatatttt atacaatact tacaattttc ctatttatcc aactaacatc
     8581 ttgcagttct caagaagtaa aagaagaaca accagatgat attttagaag aagagcttag
     8641 agcagaagca gagccaatac gtgaaattag agaaagggat ttaaaagaag aagtaaaacg
     8701 tcccatgagg aagaaataag gattcgtagt tgttaacatt tgaaaaaata ttcctttccc
     8761 tactacctat ccctttttct attctattat atcagagata cttttttcgt gggaaaagat
     8821 atggcattta ccttgatgca tttttatacg gtgttttaca agcggcgagt ttactctttt
     8881 tatttccttt tctagatgaa tttgtaaata ttgatgctaa aatttttacc ggctttttca
     8941 gtgctgcatt tatagagaaa acaacagcat tcttattttt atactatcta ttaaaaaaac
     9001 aatattcaag attaaatgta aacgaaagcg tttgttttgg aatgttttta ggaattggct
     9061 ttgcagccct agaaaatgtt ttctatgcaa tccaagttac ttcttccact atttttctca
     9121 gaacctttac ctctgtatta ctccatataa caacctgtgg aatgattgct tattatttag
     9181 ctctgaagca agcttataat acatctttaa ataaatctat ttacacaacg tatgcttata
     9241 tcctgccact catttttcac ggactctatg attcatgtct tctaattgga aatgaaatta
     9301 catatataat tggtccggaa cttgtatttc tcattttagc tttagaatat atgatggcac
     9361 gtgctttgtc tcttcccaac acagaagaac tgcatgaaaa gaaaatttct ttagaggact
     9421 gggagtcgat acaattacaa ccacaatatg aacgatggat catccgatct atgggcaaaa
     9481 aaaatgctga atttgttcct tttttttcta tcactattag accagagaaa ggatcaataa
     9541 ttgtattact aacgttaatc tctattgtat tctacttttt ccaaactcag atggtattat
     9601 tctttgattt aaaattaagt gaatatgagc taacaacgct cttcgcaatt tatcccttag
     9661 ttcttgcatt taatttgttt ttacttgggg caatcaatcc tgagtatttt aaaaatagta
     9721 tcataagcat tccaattatt atggaagtta acgttattgg tggtcttgat tcaaagtcaa
     9781 tggttggttc agaaataaca gcatacaaca gttttcttag aacatttgat acacttccta
     9841 ttggacatga actagaaatt tcttatcttt attcagattt taaatctcct ccgatcaaag
     9901 ggaaaatcat ttgggataat cacgaagatt ttgaaaaacc aatgggaagt attattcgat
     9961 ttgaagaaac tccaaaagga tttatccgtt ttttagttca atataacctt tataaattag
    10021 gaaaaggaat tttatataat ttaaaatttc caggattcaa gaagattcga aaactttttg
    10081 taaaagaatc cacggttatg gaacacaata attattatac cgctggaacg attctattca
    10141 aggaaggaga atcaggaaag aaattttatc tactcaaaaa aggaagcata gaaatctgta
    10201 agcatacaag tgcaggtgag aaaattactc tgagctttgt ggagtctgga gaaatttttg
    10261 gagaaatggc aattcttgga aatcagccta gatctgcatc tgcagtctgt atgacaaact
    10321 gtgttgtcgc aacggctgac ggagacaatt tagaggcttt aataaaggga aatcctgact
    10381 tttctctgaa attgatacag actctcgcta ccagaatcag ttattctgaa aaactcttaa
    10441 aaagtagaat tcaagaattg gaagatgcac tcaatcatga gaaagaaaaa aatcataaac
    10501 tggaaactaa agtatccgaa atgattccaa aacctgttga tttaaaaatt tctcagcatt
    10561 caaaatttag aaagcgtcgt aaaacttaat tgataaagtc ttgaaattaa tcttgaattt
    10621 tgcaagtcta ttcctaaagg aacattgttc ctttaggaga aaaaaatgaa aagaaaaata
    10681 ccaatatctc tattaatcct attctttgct cttatttcct gcgatgaaaa gaagaagcaa
    10741 tatataccaa tacaatccgt cgtgcaacaa aatgttccaa atacctccac aaatggaagt
    10801 tcgggaccta cagtaagtct accaagcgca ggtggcagta atacagattt atctggagtt
    10861 acacctggaa caactctaac caccactcct gctggaaata ccccttttac ttattcaaat
    10921 aatacaacaa ttcctgttga ctcaactgtg acgaatagtg aaggaactcc agttgctgga
    10981 gcgatagtta caatcacaaa cccagaggct aatgatgttt tactgcaaca agtaacaaac
    11041 agtgatggaa aagttacagg ttctattaca gttccaagca caacaacatc agttagttta
    11101 acagttacat caggtggaac aacttgtcag ccagttcaaa ttcctctaca agtagttaca
    11161 actcagaatg ggaatacgac aatttcccaa gtatcttcta ttggcaatat tgcaattcca
    11221 attgcgaacg ttggtgggaa ccaaactgta gctgaccgag ataaggatgg aataaatgac
    11281 gatctagatt tttatccaga tgattccaaa aaggcaacca aaattcgttt tccatcttct
    11341 agtgtaaata caattgcctt tgaagaccta tatccaaatg cgggtgatgc agacttgaat
    11401 gattatgttg ttcagttttt cacagaagaa gatttaaatg cagaaggaaa gattgttgaa
    11461 attagaggag cttttcaaca tgttgcaaga ggtgccggat atcgtcatca gctgaaaatc
    11521 ttattaccaa ataatttaga tttatcttat gaaagcacag tcacagacgc atcaggagta
    11581 gcacagaata ctagtatagc aaaatttact ccgacctcat cccaattaga ggctggtttg
    11641 attattcttg gaaacagtgg aactaccatt tcttcaccga actcagagtc atctcaaact
    11701 tatgcgccag ggcatatctc aaaatttaaa attgtattta atactcctgt agctagaaat
    11761 gtgatcgggt cttcaccgta taacgtttac ctgcaagtaa ttgatactgg aaaaaatatt
    11821 ttctttccag gaaagtattt tgatgcaaat ggaaaagatc tttatatgga ttcaaatggc
    11881 tttccttggg caatacatgt tccgggtgta tggcaatggc cactagaaag aaaagacatt
    11941 cgaaacatag acaatacagg atatcctaga tttaattctt gggcaacatc gaatggcttg
    12001 aatgataaag attggtatac aactattaca aatggcaaag tttattctat tccaacaacg
    12061 cctagcaatc tacttgctta cttaaaaggt gtagatagta acataagtgt ttatcttgca
    12121 atgtcacttg catttttagg gattatattc ggattattga ttcgaaagaa actttcaaac
    12181 gttagttcaa cctaaatata aaaatacctt gaatagaaat acttttaaga attttattca
    12241 aggtattctt tgttaaatca aataagttca ctaaaaaatt tacagattaa aaaataggaa
    12301 aaattcgtag ggtttcccct acgatgtaaa attgttcagg gaagattatt ttttttctac
    12361 agtggagact tttactgtcc agtaaagcgg acaaaactta atagctgcag ttgctagtaa
    12421 tacaactcca atcaaaccaa accaggaaga aaaatataaa ccagcagcaa tgatccctag
    12481 accgagaact actcgtatga tcctatccaa gcttccaaca tttactaaca taatcatctt
    12541 ctataagtta tttaacttct ggcatatctg atagaccacc accgttgact acattcttaa
    12601 atccattggc atctagaatt ccttttgctc ttccagatct tccacctgat ctacaataaa
    12661 caacgatttt gcgctcttta tctccaaatt cggataacct agattctact tggtcaacag
    12721 gaatattttt tgcatttgca tagtgaccag aagcatattc ttctccactt ctcacatcta
    12781 caataagggc tccacccttg atgtaatcga taacatcttt cttatccacg gctccaactc
    12841 ctttattcaa aaatctcaag gcaagtaaaa ctccaaagac tcctaccgcc agatacatta
    12901 aattgatgaa attcattttc atatatatac catacggggt attatataaa aatagcaagc
    12961 attatttttt tttatttgac ctttattgca gaatggttat tttgaatcaa taatataaat
    13021 tttaaataga atcctcttaa caattctgat tttaatacag aataaggtag gtgtttatga
    13081 caaaacaaag aacagattta atttccagac tcagccgaat tcaaggacaa ttggaaagta
    13141 taaaaaaaag ccttgaatct gaaacagata tagaatgtat taaaactatg catctgatta
    13201 aagcagcgaa caatgctctg aaaaaatttg gagaagcata cgtttccaac catatggatg
    13261 agtgcatgag cggaaaaact ccgaaaaaga atattgaaaa agagttaaaa tcagtcatag
    13321 aggcagcttt tactctttga taaaaataaa gacaagtata cacatatatt ttttgtcctt
    13381 cctgacagga attgtgactg gtagttttgt aattcttttt tcaaagctat tggaatattt
    13441 tgagcggcta ctctcattta accacagttc cttttataaa acattttcct tcgaatttaa
    13501 ttttctttac cttttattac cagcaatcgg aggcttactt gttggtttaa taacacatta
    13561 tctctgcagt gaggcaagag gaaatggaat tgatcaccta ctcgatgcat ttcataatca
    13621 gaacggggaa atgcgcggtc gtgtttcgta ttacaaagca atcgtaacaa tcataactct
    13681 atcgacggga ggaagtgctg gaaaagaagg tccttccgca caaatcggag ctagcatagg
    13741 ctcaaaaatc aataatgttt ttgcaggagg tgcaagagca agaagaactc ttttgctcgc
    13801 aggggctgct gcatctctcg gaacaatttt tcaagcaccg ttaggcggcg cgataacagc
    13861 aattgaaatg gtatatagag aagatattga aagtgacgct ctcataccct ctattatttc
    13921 ttctgtttct gcttattttt tacttagctc ttttggtctc gtgcatagag aatactttgc
    13981 tttgcaagaa ttagctccta aatatctaac tatttaccca atactaggat tgatttcttt
    14041 tggaatagga tttgttttag tcaaaataat ccaacgacta aaagcctttt tcctgaaatg
    14101 gaaaattttc cctcccttta aaccaatgat tggaggtttg atctgcggag tcatttatct
    14161 tttttttcct gttgtttcgg gaacaggaga atcctatatt gagaactttc ttttcaagcc
    14221 tgaaacctat acagggattc ccaaactcga attgattcaa tttgttttat ttgttgttac
    14281 cttcaaaatc tttgcaacaa caatcacgat tgcatctggt ggatcaggcg gtatctttgg
    14341 tccttccctt tttctgggag gaacaattgg aatgtttgtt gcggaagttg caggattatt
    14401 ctatacttta gaccccgatc actatcgcag ttttatatta gtagggatgg gttcctttta
    14461 tgcaggaata gctagtgcac cgatagccgc aatgattatg atatgtgaaa tggtaggaag
    14521 ttatgtttta ctccctcctc tcatggtatg ctccattatt gtatttcttc tatcaaaaaa
    14581 gctcaattta tattcaggac aagtgcaaga tcgatttcat tctcctgcac atcattggga
    14641 tattcaaaca gatattttgc ggaaaatgcc tatttaccac taccagacct atttacatcg
    14701 atctgctatc gcaaaagaaa ctcaaccgat tcaatttatc aaaagacgcg ctcttaaaag
    14761 agatgaaaaa gactttatta ttttaaatga atcaagcgaa tatgttggga taatctcatc
    14821 cctagaattt atgcgacagg atattaatcc aaaatcccca gtatatcctt atattgagaa
    14881 aattcctgct atttcaatca gtcaaagtct tggtgatgca ctagaacttc ttcttaaaca
    14941 caatcttgac aaagtcgctg taatcagtaa ttctagccgt ttccttggat atcttcgtta
    15001 tcaagatata ttagaactct atttctcaaa actaaaacga taactgctaa gatatcgatt
    15061 cttaaaagaa gagataagac aaagtaatga ttgagaaaat ggatccgtaa aaaattttct
    15121 ttttagtcat tttaatagaa ggattgcgct attctaccta atatctattt ctaacaaacc
    15181 tatcaaatat attttgaatt tattgcttat aaattgagaa tatatgcttt cagtttcaat
    15241 gttcttgctt tttttatatc gatgtaatta tagtctattt tgtaagattt aatcctttca
    15301 tccggagatt ttctttgaaa tctaattcat ttatactaaa aatttttcta ttactaggct
    15361 ttattctttt gtatttgcaa ataaactgca aatccaagga ttgcaactca gcccaaacga
    15421 taatcgataa agttccattc attgaaaaag caatagattg tcagaaagaa gacaattcat
    15481 tgctgcttgg attgattgca attggatcaa gaagttccac tgcaagtatt tccactgctg
    15541 ccacttgtcc tgtttctggt tcactgagca atccaagccc agtgaacggc tctagcatta
    15601 ctttgacagg tgcacaatgc acaaactctg caatccagtc tacgattaca aatggaggta
    15661 acattacttt aaattgcagt ggaacaattt caatttctag tgaattaacg gttccatcga
    15721 atggaactgt ttttataaca ggagtaaatg gctcaggaac taacggtaaa gtaatattag
    15781 atggaggtgg tatttctagg attatctatg gaaatagtag ttctaagatt attgtaaaaa
    15841 accttcaatt tcaaaatgga aaatcaaaag agactgcaga ttcaacggga gctttaaata
    15901 aaaatgcagg gggcgcttta ttcaatggtt ataaaggcga aatgactgta attgattgtg
    15961 attttttaaa taatgtcggt ggcacaaatg caagtggaaa taaagaagaa ggaggaggtg
    16021 caatttacac aaagtcattt ggtaaactaa cgatcgtaaa tagctacttt tctggaaata
    16081 ctggaggtct aggtggagcg atcaataatc tattgagcaa actaacaatc attgatagta
    16141 attttacttc gaatagttct ttagtagaca attattctgc aggaggatat gggggagcta
    16201 tttatacgga tggagctgga cttggaggag tcaatggagc aaacaatcaa gcatctctaa
    16261 atcctccctc tggattgggg aatataacga tttgccgcac tgtattctca aacaatactg
    16321 ggaaagggca aggaggtggg accttcttat atggctatgc tccggatgta atcactttag
    16381 atagaattgt ttacgataaa aattctgtta ttaaaaacgg tgcaggcgat gctttaggag
    16441 gaggacttcg atcaggaaat gcggacacct atatttctaa ctcgtctttt acggataacg
    16501 ttgctttatc acagggagga ggcttttgga aaggcgagtc tagtttgctt tctattagca
    16561 attctacttt tgtaaataat tatgctgttg agacaggctc aacgacagct gcctctcttt
    16621 caaatagaaa taatactggt ggaatatgtg gagcaatcaa taatgcaggg agttcctctt
    16681 ttgatatgac aaatcttact attgtcaata atcatgcagg atttatgagt ggagcgatat
    16741 gcggtagctc aaacagtgcc tcgaaatcta ttatagctta taatacggct tataataatg
    16801 gtaattcttg gaagaagggt caaaattgtg caggaaatca aattgtaaac aaaggatcta
    16861 attttgaatt tccaaataag aatacaactg attctacctc tgataatatt acttgcttta
    16921 actctacaat tcaagatcca ctctatcaat cttcttcaaa cctaacttac acaaactcag
    16981 ttgtgtcccc tattccactt gcaacggtaa atgactgttc aacgcaagtt ggatcttcgg
    17041 gaagaacgat tgctattaaa aacatagcac ttcaatctgg tagtcctgca actggttcgg
    17101 gtgcaagctg tccctaattt gtagaattag acttttcaat ctcctttctg caaatggcaa
    17161 ggggattaaa gatttatttt ttctaagaaa agcttttgga atgtttttat aaattctaat
    17221 tccttttctt cgctgatgtc gaatccaagc tttttcagag acaccatatt cgccgcatcc
    17281 tgcccacagg gatgaatcaa ctgaaataca tttaaatcat ttcgcaaatt gagagcaact
    17341 ccatagcttg tgaaaaaaga tttaaaataa actcccatag aaacaatttt ttttattggg
    17401 tctttttcca aataaagacc aggtctcatc gcatccgaga caagctttaa ttgccaaaca
    17461 gagtaaactg attcgatcat agagttcgtt agaatactta agtattcacc aatgttttta
    17521 cttctctttt tcaaatcaat atgaggataa atcactaatt gccctgtttc atgtgcggtt
    17581 aaatcacctc cccgctcaat cctgcaaaac tcaatttctt ttttggttaa atattcttca
    17641 ctcacgagta gattttgaat attgtagttg ataccagccg ttattgttga ggtatgctct
    17701 aaaaagataa ttatttctct tctttttttt ctgaggagag attgtaattg aatgtatcga
    17761 ttatagggga cagaatttaa tttatttgta aatttcattg gctatggaaa aacttctgag
    17821 ttcagtttat tatattattc aaaattttcc ccaaaaattg gataggaaaa atttgtctaa
    17881 gcttctctat tattcagatg gagtgtattt tcaaagacat aaggaaatga ttacaaaaga
    17941 aagatacatt cgactcgaag atggacctga acttatcaat ctgaatcttg ccattataca
    18001 cctacttgga aatggaattc tttctgtaga acctgacatt acggctatct ccgatagaat
    18061 cgatcgtttt ttcttaagat ctatttctac aatgaatctt aatttggaaa aagaagaaat
    18121 ccgaatttta aacaaagttt tgaaagcctt caaggaagga gtcaaaaacg aagataaaca
    18181 atatccaaat ctgtatgaaa actatgtaat tacccctatt ttcagtgaga ttattttttc
    18241 agaaaaaaca attaatacaa aaattcatgt ctttaagaaa aaaagtcttt taaacctatc
    18301 tggaaaaata tttagagtat tattcgaatc ctaatgctaa t
//
LOCUS       DAOQOS010000111        18049 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_1558_length_18049_cov_5.893353, whole genome shotgun sequence.
ACCESSION   DAOQOS010000111 DAOQOS010000000
VERSION     DAOQOS010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 18049)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18049)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18049
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1558_length_18049_cov_5.893353"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1077
                     /locus_tag="PLF66_19640"
     CDS             <1..1077
                     /locus_tag="PLF66_19640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HMZ66314.1"
                     /translation="IYYKYEGVSPAGSHKPNTAVPQAYYNKKEGVKKITTETGAGQWG
                     SALSFACNIFGIDLEVFMVKISFQQKPYRKSLMNTWNARVYASPTDMTESGRKILAAN
                     PDSPGSLGIAISEAVERAVQDPDTKYALGSVLNHVLLHQTIVGQEAILQMEKAEDYPD
                     VIIAPFGGGSNFAGLTFPFLRHKLKEGKKVRCIAVEPASCPKLTKGEFRYDFGDSVGL
                     TPLIPMYTLGHSFVPASIHAGGLRYHGAGAIVSQLLKDKIIEAVAIQQLECFKAGVAF
                     AKAEGILPAPEATHGIAEVFRQAEEAKREGKSRTILFNLCGHGYMDMTAYDDYFAGKL
                     TDHEFSTDEVKKSLEEIESYPKAG"
     gene            1770..2666
                     /locus_tag="PLF66_19645"
     CDS             1770..2666
                     /locus_tag="PLF66_19645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="general secretion pathway protein GspC"
                     /protein_id="HMZ66315.1"
                     /translation="MKLLNFIFKRKFLLLVFSLILLGFSFALSFRMLLIQFLNQASFS
                     FPHKTLERKQTESNSTFDLSKIDEIVEGNLIRGNTQVLIENIPNEKRPTELDLEIGLM
                     IVRGIVSGNSSFARVLVESRNSESKDYKLGEKIGDLKILQIQEHAIILGKSTYSFRVE
                     VGETIQFAFQKLNTNIESSSRDTSQRIAATQHKPVILSKTNIDRILKDPSKIYLGASF
                     GPNLVDGKIDGMKIQTVSPSHLFSALGAKSGDIIKRVNGMPLGETEKMLEIWSAIKVA
                     ERIDVDLERKGEIHSYIFLIRN"
     gene            2797..3726
                     /locus_tag="PLF66_19650"
     CDS             2797..3726
                     /locus_tag="PLF66_19650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002152178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general secretion pathway protein GspC"
                     /protein_id="HMZ66316.1"
                     /translation="MNSILLRLQKNSFYILVPIVLFFAYSFAYLIRSILIAFLNPAQK
                     ATIQITRNSGLPTDNQIPKAVSIYEDMVEGNLIRGKKVVDLPQDPAKMGANAPATISE
                     EVPGADETVLTGALSGHISFARATFKEKDKEEADEFKVGEKIVGYKIKSIADHYVVIS
                     KSGLNLKMEIGQSIKEAKTVYEEMMAKKGSAAIDDGVGPSAETKTIPISRTDFEKYMK
                     NPADLYKDARFGPNLVNGKIDGYKIYQVAVSHIFYQLGARNGDIVKRVNGMPLNETEK
                     MLEIWAAVKNAQKVTVDIDRKGKIITYDFHIRN"
     gene            3751..5496
                     /locus_tag="PLF66_19655"
     CDS             3751..5496
                     /locus_tag="PLF66_19655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008591540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HMZ66317.1"
                     /translation="MIKYTLLLFLFLVQIQLLAQAANPTGTEPKESEEDKKHNDNAGK
                     LSKEKDGNIMFTADWRNAELKDFLKGMSAIIRKNILVDDSVKGRKITIISQKKVNVKD
                     AYPFMKSVLESQGFGLVEENDIIKVVRTKDALAKSGIVRIGREPISEDELKSNKIITQ
                     VVPIYQASAGELEPLLKRVTSPDTDIIVYRLTNTLVLSGSSSDISKLLKLIERLDQRN
                     DGPGAIASAGDIHIYTLEYNEAEKLAAVLTKLDNPVVEAKVVDPSKPATTPKGEKIKA
                     VAHKDSNSVIVTATKEEWNEIQRIVKILDLPRKQILLEVLIVELTSNDLNDFGIDWRF
                     RQTAFGQFNSGLGAQSGILTKDGKLNPNANTLTGFSLGFLKPGSPQIIGILNANATNE
                     NFNVLSAPQILTIDNQEAEINVGQDVPVRTQSRNAGGGGSQAVTVDNYEYRPTGIKLK
                     FTPHINKNDKITLELYQEVKNIAGLPEQGGNPIFNKRDIKTTITVDNTQTIVIGGLIS
                     NDKQKRLTKIPVLGDIPIIGNFFRRVTSKINRTNLMVFLTPHILDNRAKADRITIEKR
                     MDQEKQEEEREKKLR"
     gene            5493..7169
                     /gene="gspE"
                     /locus_tag="PLF66_19660"
     CDS             5493..7169
                     /gene="gspE"
                     /locus_tag="PLF66_19660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015680873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HMZ66318.1"
                     /translation="MRKSLGEILLNNGTITEKELNESLKTQQKNQLPLGQIIQKKGFA
                     GEVDIMRALAELHHLDFIEKIEFSGYEEIYHSIPYKLIQKSKIVPVHYENKTVTVAVC
                     NPSDLHPMDEIKFFLKGQNVKFVLAPEPEILRIIHTHFDKTASDAKDMMEEMVDGELS
                     EFSDLESDALDLTNEAPIIKMVNVILSQAVTERASDIHIESYEKSVVVRYRVDGILHK
                     VLSPPKSYQAGIISRIKIMSNLNIAENRLPQDGRIKLKLTGKDIDIRVSTIPCQYGER
                     VVMRLLNKTDQKYSINTMGFSEDLLEKYKKLIYHPHGIILVTGPTGSGKSTTLYSSLS
                     ELNTPERNIITCEDPVEYQFDGISQMQMQDKIGLTFATGLRAILRQDPDVVMVGEIRD
                     EETARIAIQASLTGHLVFSTLHTNDAASAVTRLTDMGIESYLITSTVLGFVAQRLVRV
                     NCKSCLKPYTPSDKELDTVGISRSRLIDGKLKRGEGCPACMGTGYKGRMGIYELLVMN
                     NDIKAVILKGGDATAINEIAIKTGLVPLRQYGIQKAIEGFTTLDEVLRVG"
     gene            7178..8404
                     /locus_tag="PLF66_19665"
     CDS             7178..8404
                     /locus_tag="PLF66_19665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004780404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HMZ66319.1"
                     /translation="MALFTYVAFNKSGKEVKDIIEANNLQAARNKLKAKGLYVRVIKE
                     DAEKKERELFPFLAKILYRIPRKEVGLFVRQLGTLLGAGIPLDRSLNNISEQIENRNF
                     KKVIMDMKAGITEGLSLSQSMERYPDVFPDQYSSLISVGEKTGEYENTLIRLADLEDA
                     SNDLKSRVQVAMIYPMIMGMVSLFVAIFLLVVVIPQIQELFSQFEAELPLITRVVIGI
                     SNAIINFWWLMIGGVVGGSTAFSKWKSSEEGKKKWDKFFLKVPVFGTLTKKVLISTFA
                     RNLSVLLTNRVPLIVSLSIVAKVVGNYVFQVEILAAIDKIKEGGKMSDSFQGSTILTP
                     MVLGMISAGEASDTVPKMMDKLSDIFEKEVDSAIKSMTQSLEPIMIIVMGALIFTIMA
                     AIMTPMYKLTQEIQNL"
     gene            8416..8904
                     /locus_tag="PLF66_19670"
     CDS             8416..8904
                     /locus_tag="PLF66_19670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004460677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspG"
                     /protein_id="HMZ66320.1"
                     /translation="MKLNRFSKLSKKLKRGMTLVELSVVVLILGAIIALVAFNINPGE
                     IQDQTAALKLKKDAQELQIALEKYADAYGKMPSEDQGILALVEKPTSGDVPENYKPIL
                     NKKNAVLDPWKTPYKLKVDSNGDYGIFTLGKDKKEGGEGKNADFNIMKEDEYPKEFQK
                     NK"
     gene            complement(9129..10349)
                     /locus_tag="PLF66_19675"
     CDS             complement(9129..10349)
                     /locus_tag="PLF66_19675"
                     /inference="COORDINATES: protein motif:HMM:NF027964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HMZ66321.1"
                     /translation="MKKNVLIIGAGGVANVAAHKCAQNNDILGDICIASRKIDKCNKI
                     IDSIQRKGNLKDPSKKLHAKQLDAFDVAATVKLIKETNSEIVLNLGTAFLNMSVLEAC
                     LEAGVTYMDTAIHEDPEKVCEDPPWYANYEWKRKDRCKERGINAILGIGFDPGVVNAW
                     AALAVKHHFDKIDTIDILDVNAGSHGKYFATNFDPEINFREFSKVWTWIDRKWVEKPM
                     HSEKWVYDFPIVGKQPVYLTGHDELHSLSKNIDANSIRFWMGFGDHYLNVFNVLRNIG
                     MLSEKPVKTAEGLEVVPLKVLKAVLPDPGSLAPTYTGFTCIGDLIKGTKDGKRKEIFI
                     YNSCDHAACYKEVESQAISYTAGVPPVAAAILVAKGIWNPKTMVNVEELDPDPLIELL
                     DKIGLPTVIEDRTP"
     gene            complement(10366..11478)
                     /gene="nspC"
                     /locus_tag="PLF66_19680"
     CDS             complement(10366..11478)
                     /gene="nspC"
                     /locus_tag="PLF66_19680"
                     /EC_number="4.1.1.96"
                     /inference="COORDINATES: protein motif:HMM:NF014803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0008216 - spermidine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxynorspermidine decarboxylase"
                     /protein_id="HMZ66322.1"
                     /translation="MTEADLETPYYLIDESLLLKNLEKIALLRQRSGAKSVLALKCFS
                     TWAVFDLMKEYMDGTTSSSLYEARLGKEKFGKEVHAYSVAWSEKEIQEVKEFATKIIF
                     NSISQLNQYYPHFQNIPIGLRINPGKSHSDYDLANPTRRYSRLGAIDKTEIEKVSHLL
                     KGAMFHCNCDNDNFESFSSILDHIAKEYEDLLHKLEWVSLGGGIYFTKSGYPFDSFTE
                     KLTEFSNRFSVQVYLEPGETAITKSGFFVTQVLDIVKNEIDIAIVDSAVETHMLDLCI
                     YRLEAKMDLPQVGNHKYMITGRTCLAGDIFGTYTLPEKLEIGSRVRFSDAAGYTMVKK
                     NWFNGVQMPSIAVKRLNGKIELIRKFTYLDFLNNLS"
     gene            complement(11572..12519)
                     /locus_tag="PLF66_19685"
     CDS             complement(11572..12519)
                     /locus_tag="PLF66_19685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66323.1"
                     /translation="MAKDLEETSTKKDEVEEIEETVEDDLGFEEESTSGELSWKQKLI
                     LINSGIIGFILFIVLLFPYEELVKTYISKFALENNFVLEFRKLNLSFFSTKTIDHLLY
                     QNKDNLEVRAESIDIDTSLIDLIKRNFKGNIKVSSLNLETNDYAIKIGSLNIEDSSLT
                     GFDRDLPNIIGSINLQVFDGKILRSPVIPMIDSLQGVTIKNILLNIKKSLNSNRLNIE
                     KGVFTLSIAKITISGYIELSPVAIKASTLNLKICPKLSEKYSAEREDIASSLQLIIKD
                     SPDGCLPLQGTIGDPKFNIPGMQQPSTTPSVLSPMPQTP"
     gene            complement(12519..13034)
                     /locus_tag="PLF66_19690"
     CDS             complement(12519..13034)
                     /locus_tag="PLF66_19690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66324.1"
                     /translation="MLNKLDEREKLFVIIGVLVFVIVLFGLVISKIMEKRTNLTESLS
                     EARGNFARLDKAIQDYNYYRSLKSGDEIDLTSIYAKLDQILIRYGLKDRVLTQKDTST
                     PIKKEYIKITIDISLRSVKLPDVFKMIYDIEVMKQINSKVDYMNFIKPLPGKEEYDVN
                     IRFSSFTKVKK"
     gene            complement(13024..14664)
                     /locus_tag="PLF66_19695"
     CDS             complement(13024..14664)
                     /locus_tag="PLF66_19695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66325.1"
                     /translation="MYYTQYLAIDYGTTHIKGILFRQVLGSVTILRHETLQIVSLPEN
                     PDDEYEYNIVRFIQSFFPEESNFLINVAMDNTFIRDLVVPLNNEKAIREILPFEVENV
                     VPFPVETMAVQGCIWWIGNEVSNVVAFSVHHNEVEKTFKPFMKNDIQLSCISNDAYSL
                     SSIIRYHHAKTISEKVVGQLDIGGKLSIFNVNSEGLLSHSRFVSGGGYFVTEKIASLL
                     KVDFNRAEEIKLNFNFSFEIIEEEFKLEFIKQNHITAEQFDGIIRIIMDSLDQIGDEI
                     IKSIYALAPIHRPSVIYISGGGSGFKDVDSYLSKKIGTPVIRYPFLELENALFTTALG
                     MGYHNRLKNTDKVNFLTPEFAKMMRNTIRLDAFKPHIIIAGISFFILLTVFIVQMVID
                     TRKLNEYEAELREKFKQGFGVEVPEDEDVMSLAQNKVKEENKKSEIVRLFLNKENILE
                     LLLDLNQNFPSKDFNFVLDQFTLDGNLVSIYGRVNEFQDIGAIQSSLEKSQKFKNIKI
                     VNKNLITGVPSLKVRFKIELEILATPDKNTKKEEDNAE"
     gene            complement(14668..15804)
                     /locus_tag="PLF66_19700"
     CDS             complement(14668..15804)
                     /locus_tag="PLF66_19700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016548017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspK"
                     /protein_id="HMZ66326.1"
                     /translation="MVLFLVAIIGGSSFIIANQYVSDRMVDFKITRSNADGFRAFLLA
                     RAGMQGGLGALKKVPEEVLYQSGIAFNPPPIPMAGGTIYYRISAEDGKFNINDLIKAY
                     DNLPNLKVQEMITKLFEQLGIPRDRVYPIIDWIDDNSEEMGGGAEVYYYTNLKPPRKI
                     KNAPMYSLSELTALKGWNRKMVYESLKDPIKEKNVSKDFMSEEEKLLVTDADYVLSNN
                     ITAYVPFKDTGDDRININAAPYHVLMSISEFMTRQAAMRILKYKLEKGGYIKEITDLK
                     NFAEFQIKTTTLGSSTSPQTGAANNNQPPPPGNNAPGANQASQALTLFDEIAGQGTAV
                     STGRVKTKGEIYKVIGVGTVGSATRRVTCIFDLTNDQILFYSED"
     gene            complement(15818..16471)
                     /locus_tag="PLF66_19705"
     CDS             complement(15818..16471)
                     /locus_tag="PLF66_19705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspJ"
                     /protein_id="HMZ66327.1"
                     /translation="MKIFRFPKNIRFRKGLTLIELSIVVAILGVIFSGIFGAYYAALK
                     ISRESDPRGGTTRKDIFFALENLRSTFSQTYYIQGHRRLIFEGKNENTAGGRADIVVF
                     AANHTNSEETGTPAVREVSYFLRQMCDDCEYYYLMRREDEMVDADPRTGGTEHMILSH
                     VRSFQLKYSQRGDKWQDEWSSADLKKIPKLIRIELIALVGDTELKYETLAYPGLYFK"
     gene            complement(16476..16991)
                     /locus_tag="PLF66_19710"
     CDS             complement(16476..16991)
                     /locus_tag="PLF66_19710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004460680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HMZ66328.1"
                     /translation="MKTNKLRQGFTLIEISIALAIAASAIVFTFSLISKGLSLQRQSV
                     TLTNAVFLAKIKMAQIDASPKLETTSSKGDIPGYEGYRYETEIKEEELDLLKLSEQGG
                     NKKKTASPDDLLGSSANSRMDDLLKKRGKTTGSKTGGLIKVFRITVKIFYPVGYGENV
                     YTAETLKSSTF"
     gene            complement(16978..17532)
                     /locus_tag="PLF66_19715"
     CDS             complement(16978..17532)
                     /locus_tag="PLF66_19715"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HMZ66329.1"
                     /translation="MKVRRGMTLVEIVVVVSILGLLMALMVSSVSSLIRPSSKDTADK
                     IKAGLYYAYQTAIISNNTVLFDIDMEKNKFTAAKLIRSEEGLKEKKVLEVALPSHNKI
                     VNVTDLRGVKYETGHLIIPFTHDGVAGDYNIHIGEDANNIYKSILLYRYNGKIVVKNG
                     EVNRSSGAGQDNTQRIKMDADEDK"
     gene            complement(17544..17816)
                     /locus_tag="PLF66_19720"
     CDS             complement(17544..17816)
                     /locus_tag="PLF66_19720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66330.1"
                     /translation="MKKLFGFLLLVIFFQCNYLTTDTECEKQKNATNRCINSFYLYCL
                     GNKTRTECDGSLQSTLFIGSLCNYKTSCTSSSEDVVKESIQPRGKE"
BASE COUNT         5738 a         3161 c         3377 g         5773 t
ORIGIN      
        1 atttattata aatatgaagg cgttagtccc gcagggtctc ataaaccaaa tacggcagtt
       61 ccacaagcct attataataa aaaagaaggt gttaaaaaaa tcacaacaga aaccggtgca
      121 ggacaatggg gaagtgctct gagtttcgca tgtaatattt tcggaatcga tctagaagta
      181 tttatggtta aaattagttt ccagcaaaag ccatatcgta agagtttaat gaatacttgg
      241 aatgctagag tctatgcatc tcctacagat atgacagaat ctggacgtaa aattttagcg
      301 gctaatcctg attcaccggg aagtcttggt attgcaatct ctgaagcagt agagagagcg
      361 gtgcaagatc cagatactaa atatgctttg ggaagtgtat taaatcatgt tttacttcac
      421 caaacgatag ttgggcagga agcaattcta cagatggaaa aagcagaaga ctatccggat
      481 gtaatcattg ctccttttgg aggtggttct aattttgctg gacttacctt tccatttcta
      541 cgtcataaat tgaaagaagg aaaaaaagta agatgtatcg ctgttgaacc agcttcttgt
      601 ccaaaactta caaaaggtga gtttagatat gactttggag attctgtggg gctaacacct
      661 cttatcccta tgtatacact tggacattct tttgttccag catctatcca tgcaggtggg
      721 ctcagatacc atggggctgg agccattgta agccaactct taaaagataa aatcattgaa
      781 gcagttgcga ttcaacaatt agagtgcttc aaagccggag ttgcctttgc aaaagcagaa
      841 ggaatcttac ctgctccaga agcgacacac ggtatagcag aagtatttag acaagcagaa
      901 gaagcaaaac gagaaggtaa atctagaacc attctattta atctatgtgg tcatggatat
      961 atggatatga ctgcctatga tgattatttc gcagggaaac tcacagatca tgaattttcc
     1021 actgatgaag taaaaaaatc tctagaggaa attgaatctt atccaaaagc aggctaatct
     1081 ctgttagtcg caaaagagat tatatctttc agagtccaaa aagtaatttt tttggactct
     1141 tttcgctcat tctatatttc tactcaatca tctccggatt gtttcttata agtgtggaaa
     1201 taataaaaga atgaaactca gttcttccat cccaaacgtc aaaagaaaag tcctatttca
     1261 aaaataaaac gatgggtttt gtcgaaatac cctgacgttc ttgtgcatcc gtatctgtga
     1321 tatatcaaat gcgattcagt caaaatcctt ctaggaaatt tcttttgtta ttttctatag
     1381 aatacaagta ttacgtttag aaataaaatt ctcgctcttt tcctctacat cctttagaat
     1441 tgagttggta tatcaatcat tcattttaaa aaataattca aatagcatga tttcgatata
     1501 acaaaaaatt gaagagatta ccactgatga taaggcgaag tcgctcgtcg cgaatactgg
     1561 gagcgaaggg tggaatgagc atagatcgct tctattctag tatcttctaa tttcttcgag
     1621 atctgcgtgc tcttcgtggt tcattccttt tttaaaattg attcccattg attttttaaa
     1681 tcaggtgaga atttaaaaaa ttaaaataca aagttttcat ccttgtgtcg attcaatcag
     1741 tctattctat gtgtgggtaa ggatgctata tgaaattatt gaattttatt tttaaaagaa
     1801 aatttttatt attggttttt agtttaattc ttttgggttt ttctttcgct ctatctttta
     1861 gaatgctcct gattcaattt ttgaatcagg ctagtttttc ttttccacat aaaactttag
     1921 aaagaaagca aacagaatct aattctactt ttgatctctc taaaatagat gaaatcgtgg
     1981 aaggaaatct aattcgtgga aatacacaag ttctaattga aaacatacca aatgaaaaaa
     2041 gacctacaga gttggattta gagataggtc ttatgattgt tcgagggatc gtgagcggca
     2101 actcttcgtt tgcccgtgtc ttagtggaat ctagaaattc tgaatcaaaa gattacaagc
     2161 ttggtgaaaa aattggagac ttgaaaatct tacaaatcca agaacatgca atcattttag
     2221 gaaaatcaac atactccttt cgggtggaag taggagaaac aattcaattt gcttttcaaa
     2281 aactaaacac aaatatcgaa tcaagtagta gagatacttc ccaaagaatt gcggctactc
     2341 agcataaacc ggtgattctt tctaaaacaa atatcgatcg gattttaaaa gatccaagta
     2401 aaatctattt aggggcaagc tttggaccga atttagtgga tggaaaaata gacggaatga
     2461 aaatccaaac tgtctcacca tctcaccttt tttctgcgct cggagcaaag agtggggata
     2521 tcatcaaaag agtaaatgga atgcctcttg gggaaacgga aaaaatgtta gaaatttgga
     2581 gtgctataaa ggttgcagag agaattgatg tagatttaga gcgcaaaggg gaaattcaca
     2641 gttatatttt tctcattcga aattaggaat tcatacagag ttttgaatct gatttagatc
     2701 tcaattctct aaagaaaaaa cgaattcaga actctttgtt attttatttt ctgaaaaagt
     2761 aagtgacagt ttgggaattg gttttagttt atcgttatga attctatcct tttacggcta
     2821 caaaagaaca gcttttatat tttagttcca atcgttcttt tctttgctta ttcctttgcc
     2881 tatctgattc gatctatttt gatagctttc ttaaaccctg cacaaaaggc tacaattcaa
     2941 attacgcgta attctggtct tcccacagac aatcaaattc ctaaagctgt cagtatctat
     3001 gaggatatgg tagagggaaa tttgattcgt ggcaagaagg ttgtcgatct tccacaagat
     3061 ccagctaaaa tgggagccaa tgctcctgct accatttcgg aagaagttcc aggggcagat
     3121 gaaacggttc taacgggtgc tctcagtggg catatctctt ttgctcgtgc aaccttcaaa
     3181 gaaaaggaca aagaagaagc agatgagttt aaagtcgggg aaaaaattgt cggttataaa
     3241 ataaaatcga tagcagatca ttacgttgta atctcaaaaa gcggactcaa tttaaaaatg
     3301 gaaattgggc aaagcattaa agaagcgaaa actgtttatg aagaaatgat ggcaaagaag
     3361 ggtagcgcag caatcgatga tggggtggga ccttctgctg aaacgaaaac aattccaatc
     3421 tctcgaacgg atttcgagaa gtatatgaaa aatccagccg atttatataa agatgctcga
     3481 tttggtccaa acttagtgaa tggcaaaatt gatggctata aaatctatca agtggctgtc
     3541 agtcacatct tttatcaact tggtgctcgc aatggagaca ttgtaaaacg tgtcaatgga
     3601 atgcctttga atgaaacaga gaaaatgtta gaaatttggg cggcggtaaa aaacgcgcaa
     3661 aaggtaacag ttgacataga tcgcaaaggt aagattatta cctatgattt tcatatacgc
     3721 aattaaaaaa taaagattaa gtttagttag atgataaaat acacattact cctatttcta
     3781 tttctagtac agattcaatt gcttgcgcag gctgcaaacc caaccggaac agagcctaaa
     3841 gaaagtgaag aagataaaaa gcacaatgac aatgccggaa aactttcaaa agaaaaagat
     3901 ggaaacatca tgtttactgc tgactggcga aatgcagaat taaaagactt cttaaaagga
     3961 atgagtgcaa tcattcgtaa gaatatttta gtagatgatt cagtcaaagg gcgcaagatt
     4021 acaattattt cccaaaaaaa agtaaatgta aaagatgcgt atccatttat gaaatctgtt
     4081 cttgagtcac aaggttttgg actcgtggaa gaaaacgata ttatcaaggt tgttcgaacc
     4141 aaagatgctc ttgcaaaatc aggaatcgtt cgaattggtc gggaaccaat ttcagaagat
     4201 gaattaaaat caaataaaat tatcactcag gttgttccta tttaccaggc aagtgctgga
     4261 gaattagaac ctcttctaaa gagagtgact tcaccagaca ctgatattat cgtttatcgt
     4321 ttgacaaata cactcgtttt gtcgggatct tctagtgata ttagtaagct tttaaagtta
     4381 atagaaagat tagaccaaag aaacgatggt cccggagcaa ttgcttctgc tggagatatt
     4441 catatctata cactcgaata caatgaagca gagaagttag ccgctgtatt aaccaagcta
     4501 gacaatcctg ttgtagaagc aaaagtggta gacccaagta agccagctac aactccaaag
     4561 ggcgaaaaaa tcaaagcagt tgctcataag gattctaact cagtgatcgt cactgctaca
     4621 aaagaagaat ggaatgaaat tcaacgaatt gtaaaaattc tagatttacc tagaaagcag
     4681 atcctactcg aagtattgat tgttgagtta acgtctaacg atttaaatga ctttggtatc
     4741 gactggagat ttagacaaac agcctttggt cagtttaact caggtctagg tgcacaaagt
     4801 ggtattttga caaaagatgg aaagttaaat ccaaatgcaa atacactcac tggtttttct
     4861 cttggattct taaaacctgg gagtccgcaa atcatcggta ttttaaatgc gaacgcaacc
     4921 aatgaaaatt tcaacgtatt gtctgctcct cagatcctaa cgatcgataa ccaagaagct
     4981 gagatcaatg tgggacaaga cgttcctgtt cgaacacaaa gtagaaatgc agggggtggt
     5041 ggttcccaag ctgtaacagt tgataactat gaataccgac caactggtat caaattaaaa
     5101 tttacaccgc atatcaacaa aaacgataag atcacactcg aactctatca agaagtaaaa
     5161 aacattgcag gtcttccaga gcaaggggga aacccaatat ttaataagcg cgatatcaaa
     5221 acaacgatca ctgtagacaa tacacaaacg attgtaatcg ggggactcat ctcgaacgac
     5281 aaacaaaaac gtttaactaa aattccagtg ctcggagata ttccaatcat tggaaatttt
     5341 ttccgtcgtg tcacatctaa gatcaatcga acgaacttaa tggtatttct aactccacat
     5401 atactagaca atagagctaa ggcagatcgt ataacgatcg aaaagagaat ggatcaagaa
     5461 aaacaggaag aggaaagaga aaagaaacta cgatgagaaa gagtcttgga gaaatcttac
     5521 ttaataatgg aacgatcacc gagaaagaat taaatgaatc gttaaagacc caacaaaaaa
     5581 atcaacttcc tctcgggcaa atcattcaga aaaaaggatt tgccggtgag gttgatatta
     5641 tgcgtgctct ggctgagctg catcatcttg attttattga aaagatagaa ttttctggct
     5701 atgaagaaat ctatcactcc attccataca aactcattca aaaaagtaaa attgttcccg
     5761 ttcactatga aaataaaaca gtcacagtag ctgtttgcaa tccatctgat cttcatccaa
     5821 tggatgagat caaattcttc ttaaaaggtc agaacgttaa atttgtatta gcccctgagc
     5881 cagaaatttt aagaattatc catactcatt ttgataaaac cgcctccgat gcaaaagaca
     5941 tgatggagga aatggtagat ggagagctca gtgaattcag tgatctagaa tccgatgcac
     6001 tcgatcttac gaacgaagct ccgattatta aaatggtaaa cgttatttta tctcaggcgg
     6061 ttacagagag agcatctgat attcacattg aatcttatga aaagtcagtt gttgtgcgtt
     6121 accgtgtaga tgggatttta cataaagtat tgtctccgcc taagtcgtat caagctggga
     6181 ttatttctcg tattaaaatc atgtcgaatc taaacatcgc agaaaaccgc cttccacaag
     6241 acggtaggat taaattaaaa ctcactggaa aagacattga tattcgagtt tcaacgattc
     6301 cttgtcaata tggagaaagg gtcgttatgc gacttttaaa caagaccgac cagaaatatt
     6361 ccattaatac aatgggcttc tcagaagacc ttcttgaaaa atataaaaaa ctaatctatc
     6421 acccacatgg aatcatactc gttacaggtc ctactggttc tggaaaatct actactctct
     6481 attcttctct ttctgaactc aatactccgg agcgaaatat tatcacttgt gaggatccag
     6541 tggaatacca gtttgatggg atttctcaaa tgcaaatgca agataaaatt ggacttacat
     6601 ttgctactgg acttagagcc attttacgac aggatccgga tgttgtgatg gtgggggaga
     6661 ttcgagatga ggaaacggct aggattgcga ttcaagcttc tctcaccgga cacttagtgt
     6721 tttcgaccct tcatacaaat gatgcggcaa gtgctgtgac tcgtttgaca gatatgggaa
     6781 ttgaatccta tttgattacc tctactgttt taggatttgt cgcacaacgt cttgttcgtg
     6841 taaattgtaa gtcttgtctg aaaccttata ctccttcaga caaagaatta gatacagttg
     6901 ggatatctag gagtagactg attgatggaa agttgaaacg aggtgagggc tgtcctgctt
     6961 gtatgggaac aggatacaaa ggacgtatgg gtatttatga attactcgtt atgaacaatg
     7021 atattaaggc ggtaatttta aaaggtggcg atgctactgc aatcaatgaa attgcaatca
     7081 agacaggtct tgttcctctc agacaatatg gaattcaaaa agcgattgaa ggatttacaa
     7141 ctctagatga agtattgaga gttgggtaag agggactatg gctcttttta catatgtagc
     7201 atttaataaa tctggaaaag aagttaaaga catcattgaa gcgaataatc ttcaagctgc
     7261 tcgaaacaaa ttaaaagcaa aaggacttta tgttcgagtt atcaaagaag atgcagaaaa
     7321 aaaagagaga gagctttttc catttcttgc aaaaatttta taccgtattc ctagaaaaga
     7381 agttggactt tttgtaagac aacttggaac gttactcggc gcagggatcc cactcgatag
     7441 gtcactcaat aatatcagtg agcagattga aaatagaaat tttaagaaag taattatgga
     7501 tatgaaagct ggtattacag aagggttatc tctttcacaa tccatggaac gttatcccga
     7561 tgtatttccg gatcaatatt cctctcttat ctctgtagga gaaaagacag gcgagtatga
     7621 aaatactttg attcgtttgg ctgatttaga ggatgcatcg aatgatctaa agtctcgtgt
     7681 gcaagtggca atgatttatc ctatgattat gggtatggtt tctttatttg ttgcgatctt
     7741 tttacttgta gtggtgattc cacagattca ggaattattc tctcaatttg aagcagaact
     7801 ccctctcatt actcgtgttg tcattggaat ctcaaatgca attataaatt tttggtggct
     7861 catgattgga ggcgttgtcg gtggttctac tgcatttagt aaatggaagt cctcggaaga
     7921 gggaaaaaag aaatgggata aattcttttt aaaagttcct gtatttggaa cactgactaa
     7981 aaaagtattg attagcacgt ttgctcgaaa tctttctgtg ctactaacaa accgcgttcc
     8041 actgattgtt tctctctcta ttgttgcaaa agttgtaggc aattatgtat ttcaggttga
     8101 aatcttagca gcaattgata aaatcaaaga aggaggaaaa atgtctgact cctttcaagg
     8161 ttctacaatt ttaactccga tggtgctcgg gatgatttct gcgggcgagg catccgatac
     8221 agttccaaag atgatggaca agttatctga tatttttgag aaagaggtgg atagtgcaat
     8281 caagtcgatg actcaaagtt tggagccaat catgattatt gtgatgggtg ctctcatctt
     8341 tacgattatg gctgctatta tgacaccaat gtataaacta acacaagaaa tacaaaattt
     8401 ataaaagagg acactatgaa actaaatcga ttttcaaaac tttcaaaaaa actaaaacgc
     8461 gggatgacac ttgttgaact ctctgttgtg gtattaattc taggtgctat cattgcactc
     8521 gttgctttta atattaaccc aggtgaaatt caagatcaga cagctgcgtt gaaactaaaa
     8581 aaagatgcac aagagttaca gattgcatta gaaaagtatg cagacgctta tggtaaaatg
     8641 ccaagtgaag accaaggaat tttagcgtta gttgaaaagc ctacttctgg tgatgttcct
     8701 gaaaactaca aaccaattct aaacaaaaag aatgctgttt tagatccatg gaaaactcct
     8761 tataagttaa aagtagattc aaacggagat tatggaatct ttactcttgg gaaagataaa
     8821 aaagaaggcg gggaaggtaa aaacgctgac tttaatatca tgaaagagga tgaatatcca
     8881 aaagaatttc aaaagaacaa ataatctttc gaacgttagt gcgttaacct agctaatata
     8941 aagatttttt taccacggat gcacacagat aaacacggat ggatttaatt ttttaccacc
     9001 gacttgcact gacccatgtc gaatgtgtaa agagcgatga caccgataaa aatatcgaaa
     9061 tttaaaaata tcggtgttca tccgtgtcta tccgtggtta aatattctta catcgaactc
     9121 actttaaatt acggggttct atcttcgatt acagttggaa gaccgatttt atctagaagt
     9181 tcgatgagtg gatctggatc gagttcttct acattcacca tagtttttgg attccaaatt
     9241 cccttcgcaa cgaggattgc tgcagcgact ggaggaactc ccgctgtata ggaaatagct
     9301 tgtgactcta cttctttata acaagcagca tgatcgcaac tattgtagat aaaaatctct
     9361 ttacgttttc catctttagt tcctttgatt aaatccccaa tacaagtaaa accagtatag
     9421 gttggtgcta gagagccagg atcaggaaga actgctttta agactttgag tggaactact
     9481 tctagacctt ccgcagtctt aaccggtttc tcggaaagca ttccaatgtt tctaagaacg
     9541 ttaaacacat ttagataatg atctccgaat cccatccaga aacgaatgct atttgcatcg
     9601 atgtttttgg aaagagagtg cagctcatca tgtccggtga ggtaaacagg ttgtttgcct
     9661 acaattggaa agtcataaac ccatttttca gagtgcattg gcttttctac ccatttgcga
     9721 tcgatccaag tccagacttt gctaaattct ctaaagttga tttctgggtc gaagttcgtt
     9781 gcaaagtatt ttccatgact tcccgcattt acatctaaaa tatcaatggt atcaatttta
     9841 tcaaaatgat gttttactgc aagggctgcc caagcattta caactcctgg atcaaaacca
     9901 attcctagaa tcgcattgat tcctctttct ttgcatctat cttttctttt ccattcatag
     9961 tttgcatacc aaggaggatc ttcacaaact ttttctggat cttcgtgaat ggctgtgtcc
    10021 atataagtaa caccagcttc taagcaagct tctaatacag acatatttaa gaaagctgtt
    10081 cccaagttta agactatttc tgaatttgtt tctttgataa gtttaacggt agccgctaca
    10141 tcaaaagcat cgagttgctt cgcatgtaat tttttagaag gatctttaag atttcctttt
    10201 ctctgaatgc tgtcgatgat tttgttacac ttatcaatct tacgagaagc aatacaaata
    10261 tcgcctaaaa tatcattgtt ctgagcacat ttatgcgcag caacatttgc aacccctccc
    10321 gctccaatga tcaatacatt ttttttcaag atgttttctc ctaatttatg aaagattatt
    10381 taaaaaatcc aaatatgtaa attttcgaat caattctatt tttccattta gacgttttac
    10441 tgcaatagat ggcatttgaa ctccgttgaa ccaattcttt tttaccattg tataaccagc
    10501 tgcatctgaa aatcgaactc tactaccgat ttctaatttt tcagggagag tataagttcc
    10561 aaaaatatct ccagctaagc aggttcgacc cgtgatcatg tatttatggt ttcctacttg
    10621 gggtaaatcc atctttgcct caagacgata gatacaaaga tctagcatgt gggtttctac
    10681 tgctgaatct acaatggcaa tatcaatttc attttttaca atgtctaaaa cctgagttac
    10741 aaaaaatcca gatttagtaa tcgccgtttc tcctggttct aaataaactt gaacactaaa
    10801 gcgatttgaa aattcagtaa gtttctctgt aaaagaatca aaaggatagc cagacttagt
    10861 aaaatagatt ccacctccta gacttaccca ttctagttta tgtagaagat cttcatattc
    10921 ctttgctatg tggtctagta tacttgaaaa actctcaaag ttatcattgt cacaattaca
    10981 atggaacata gcacctttta acagatgact cactttctct atttcggttt tatcgattgc
    11041 gcctaacctt gagtatcttc gagttggatt cgctagatca taatcagagt ggctttttcc
    11101 ggggttgatt ctaagtccaa tcgggatatt ttgaaaatgt ggataatact gatttaattg
    11161 tgaaatagaa ttaaaaataa ttttagtcgc aaattctttt acttcttgga tttctttttc
    11221 agaccaagca acactatagg catggacttc tttaccaaac ttttcttttc cgagtcttgc
    11281 ttcatagagt gagctagaag tagttccatc catgtattct ttcattaggt caaagactgc
    11341 ccaagtagaa aaacatttca gtgcaagcac tgattttgcg ccagaccttt gcctgaggag
    11401 tgctattttt tctaggtttt tgagaagtaa ggattcatca atgagatagt agggtgtttc
    11461 aaggtctgct tctgtcatac tatctactag aacttggaat tgattgctat tgtcaagagc
    11521 aagttgtcga agtgtatttt acattttata aaatttttca atacaatacg attatggtgt
    11581 ttgaggcata ggagaaagaa cggatggagt tgtagatggc tgttgcattc ctggaatatt
    11641 aaactttgga tctccaattg ttccctggag tggcaagcat ccatcgggag agtctttgat
    11701 gattaattgg agactagatg caatatcttc tctttctgcg ctgtattttt ctgagagctt
    11761 tggacaaatt tttaaattga gagtggaggc ttttattgcc actggggata attctatgta
    11821 accgctgatc gtaattttag caatggataa tgtaaaaaca cctttctcaa tatttaatcg
    11881 attgctgttt aatgatttct taatattcag taaaatattt ttgattgtaa ctccttgtaa
    11941 agagtcaatc attgggatca caggagagcg taaaattttt ccatcaaaga cctgaagatt
    12001 gatacttcca atgatattgg gaaggtctct atcaaagcca gtgagactag aatcttcaat
    12061 atttaaacta ccaattttga ttgcatagtc gttagtttct aagttaagag aacttacttt
    12121 gatatttcct ttgaagtttc ttttgattaa atcgataaga cttgtgtcga tatcaatgga
    12181 ttctgctcta acttctagat tgtctttgtt ttgatagagt aggtgatcta ttgttttagt
    12241 agagaagaaa gatagattta gttttctaaa ttctaaaaca aaattatttt cgagtgcaaa
    12301 tttagaaata tatgttttta ctaattcctc gtagggaaat aaaagcacta taaacaatat
    12361 aaatccaatg attcccgaat tgattaagat taatttttgt ttccaactta attcaccgga
    12421 tgtgctttcc tcttcaaaac caagatcatc ctctactgtt tcttcaattt cttctacttc
    12481 atcttttttt gtggatgttt cttctaagtc ttttgccatt atttcttcac cttggtaaac
    12541 gaggaaaatc ggatgttcac atcatactct tcttttccag gaagaggttt gataaaattc
    12601 atataatcaa ctttactatt gatctgtttc ataacttcaa tatcataaat cattttaaat
    12661 acatctggaa gttttacaga tcttaaactg atatcaattg taatcttgat gtattctttt
    12721 ttgatagggg tagatgtatc tttttgggtt agaactctgt cttttaatcc atatcgaatt
    12781 aggatttggt ctaattttgc gtagatactg gttagatcga tttcatctcc cgatttgagg
    12841 gagcgatagt agttatagtc ttgaattgct ttgtcaagcc ttgcaaaatt ccctctagct
    12901 tcagaaagac tctctgttag atttgttctt ttctccatga ttttagaaat cacgagccca
    12961 aacagaacta taacaaatac caaaactcca atgattacaa atagcttttc tctttcatct
    13021 aacttattca gcattgtctt cttctttctt cgtattttta tctggagttg ctaaaatttc
    13081 cagttcaatt ttgaatctaa cttttaaact aggcacaccg gtaatcaagt tcttattcac
    13141 tatttttata tttttaaact tttgcgattt ttcaagagaa gattgaattg ctcctatatc
    13201 ttgaaattcg tttactctcc cataaataga aaccaagttt ccatctagtg tgaattgatc
    13261 taaaacaaag ttaaaatctt tactaggaaa gttttgattt agatctagaa gaagctctaa
    13321 aatattttcc ttgttcaaaa aaagtcgaac gatctctgat ttcttatttt cttcttttac
    13381 tttgttttgt gcaagagaca ttacgtcttc atcttctgga acttccactc caaagccctg
    13441 tttgaatttt tctcggagtt ctgcttcgta ttcatttagt tttcgagtat ctataaccat
    13501 ctgcacaata aacacagtaa gtaatataaa gaaagaaatt ccagcaatga tgatatgagg
    13561 tttgaaagcg tccagacgga ttgtatttct catcattttg gcaaattctg gagttaaaaa
    13621 atttacctta tcagtgtttt ttaatcgatt gtggtatccc attccaaggg ctgtagtaaa
    13681 gagagcattt tccaattcaa gaaaaggata tcgaattaca ggagttccta ttttctttga
    13741 taaataacta tctacatctt taaagccgga tccaccaccg gaaatgtaga taacagaagg
    13801 tctatgtata ggagctaacg cataaatact tttaataatt tcatcaccga tttgatctag
    13861 agaatccatt atgattcgta taattccatc aaactgttcc gcggtaatat gattttgttt
    13921 tatgaattct aacttgaatt cttcttctat gatttcaaaa gaaaagttaa agttgagttt
    13981 tatttcttcg gctctattga aatctacttt cagtagtgag gcaatttttt ctgtaacaaa
    14041 atatccacca ccacttacaa acctagaatg gctgagaaga ccttcgctat ttacattgaa
    14101 tatggaaagt ttgcctccaa tgtctaattg accaacaact ttttcggata tcgtttttgc
    14161 atgatgataa cggatgattg aactgagact ataagcgtca tttgatatac aactcaattg
    14221 aatatcattt ttcataaaag gtttaaaagt tttttctact tcattgtgat gaacactaaa
    14281 tgcaacaacg ttagacactt cgtttccaat ccaccagata caaccttgga cagccattgt
    14341 ctctactgga aatggaacta cgttttctac ttcaaatggc agaatttcac ggattgcttt
    14401 ttcattgttt aaaggaacaa ctaaatcacg gataaatgta ttatccatag caacattgat
    14461 tagaaaattg gattcttctg ggaaaaaact ctggataaaa cgaacaatat tatattcata
    14521 ctcatcatct ggattttctg ggagagatac gatttgcaat gtttcatggc gtaaaattgt
    14581 aacactacct agaacctgac gaaataagat tcccttaata tgagtggttc cataatcaat
    14641 tgctaaatac tgagtataat acattcttta atcttcggaa taaaataaaa tttgatcatt
    14701 tgttaaatca aaaatacagg tgactcttct agttgcagaa ccaacagttc caactccaat
    14761 tactttgtaa atttctcctt tggtctttac ccttccagta gaaacggctg ttccctgacc
    14821 tgcaatctca tcgaagagag tgagagcctg agacgcttgg tttgctccag gagcattgtt
    14881 tcctggggga gggggctgat tgttgttagc cgctcccgtc tgtgggctag ttgaacttcc
    14941 tagtgttgta gttttaattt ggaactctgc gaaatttttt aagtcagtga tctcttttat
    15001 atatccgcct ttttctagtt tatatttcaa aattctcata gcagcttgtc tagtcataaa
    15061 ttcagaaata gacatgagca catgataggg agctgcattt atattgatgc gatcatcacc
    15121 cgtatcttta aagggcacat aagctgttat gttattggat aatacataat cggcatctgt
    15181 aacaagtaat ttttcttctt cggacataaa atctttcgaa acattttttt ctttaattgg
    15241 atcctttaaa gattcataga ccatttttct gttccaaccc ttgagagctg ttagttcaga
    15301 aagggaatac ataggtgcgt ttttaatctt tcgaggcggc tttaaattgg tataatagta
    15361 aacctctgct cctcctccca tttcctctga attatcatca atccaatcga tgatggggta
    15421 gactctgtcc cttgggatac caagttgttc gaatagtttt gtaatcattt cctgcacttt
    15481 gagattgggc aagttgtcat aggctttgat taaatcattg atattaaatt tcccatcctc
    15541 tgcggaaatc cggtaataaa tagtgcctcc tgccatggga ataggaggtg ggttgaatgc
    15601 aatccctgat tggtataaaa cctcttccgg aacttttttt aaggcaccaa gccctccctg
    15661 cattcctgct cttgcgagta gaaaggctct aaatccgtca gcattcgatc tggttatttt
    15721 aaaatctacc attcgatcgc tcacatactg gtttgcgatg ataaaggaag agcctccaat
    15781 gatagctaca aggaagagaa ccattcctcc tcttcgttta tttgaaatat aagcccgggt
    15841 acgcgagagt ttcatattta agttcagtat cgccaacgag ggcaattaat tcaattcgaa
    15901 ttaatttagg aatttttttt aagtcagcgg agctccattc atcttgccat ttatcaccgc
    15961 gttgggagta ttttaattgg aaacttcgaa cgtgcgataa aatcatatgt tcggttcctc
    16021 ctgttcttgg gtctgcatcg accatttcgt cttctctacg catgagatag tagtattcac
    16081 agtcgtcaca catctgtctt aaaaaataac tgacttctct aacggcaggc gttcctgtct
    16141 cttcggaatt ggtatgattt gcggcgaaca caactatatc tgcgcgaccg cctgcagtat
    16201 tttcattttt accttcaaag atgagtcttc tatgtccttg gatataatag gtttgagaaa
    16261 aggtagaacg aagattttcg agtgcgaaaa aaatatcctt tcgagtggtt cctcctcttg
    16321 gatctgattc tcttgagatt ttaagagcag cataataggc tccaaaaatt ccggaaaaaa
    16381 ttacacctag aattgcaact acgatggaga gttcgattaa cgttaagcct tttcgaaagc
    16441 gaatgttttt gggaaatcga aatattttca tggaattaga aagtgctcga ttttaaagtt
    16501 tcagcggtat atacattttc accatatcca acaggataaa aaattttaac agtgatgcgg
    16561 aaaactttga ttagcccccc agttttagat ccagttgtct tacctcgctt ttttaaaagg
    16621 tcatccattc tggagttggc agaacttccg agtagatcat cagggcttgc tgtctttttt
    16681 ttgtttcctc cttgttcgga gagttttaaa aggtctaatt cttcttcttt gatctcggtt
    16741 tcatatcgat agccttcgta acccgggata tccccttttg aggaagtggt ttctagtttg
    16801 gggcttgcat cgatttgagc cattttgatt ttggcaagga ataccgcatt ggtaagtgtg
    16861 acagattgtc tttggagaga caagcctttg ctaattagag aaaacgtaaa tacaatagcg
    16921 cttgcggcta tcgctagtgc gatagatatt tcaatcagtg taaatccttg tctaagttta
    16981 tttgtcttca tctgcatcca ttttaatacg ctgtgtattg tcctgaccag cgccagagga
    17041 tcgattgacc tctccatttt taacaactat ttttccattg taacgataaa gcaagataga
    17101 tttgtaaata ttatttgcat cctctcctat atgaatatta taatctcctg cgactccatc
    17161 atgggtaaag ggaatgatta gatgacctgt ttcatactta acccccctta aatcggtgac
    17221 atttacaatt ttattatgag agggaagcgc tacttctaat actttttttt ctttgagtcc
    17281 ttcttcgctt cgaattaact ttgctgcggt gaatttattt ttttccatat caatatcaaa
    17341 taaaacagtg ttgttggaga tgatggcagt ttggtaggca taataaagac ctgcttttat
    17401 tttgtcagct gtatctttgg aggaaggtcg aatgagactt gagacactgc ttaccataag
    17461 agccatgaga agtcctagaa tagagactac gactacaatt tcaacaagcg tcattcctct
    17521 ccgaactttc attctccgga taatcattct ttgcctctcg gttgaattga ttcttttact
    17581 acatcctctg aggaagaagt gcaagaggtt ttataattgc ataaacttcc aataaagaga
    17641 gtagattgaa ggcttccatc acactcagtt ctggttttat ttccaagaca gtagagataa
    17701 aaagaattaa tgcatcggtt ggttgcattt ttctgtttct cgcattctgt atcagtcgtt
    17761 aaataattgc attgaaaaaa gataactaag agtaagaatc caaaaagttt tttcatagag
    17821 agtagagtca tcttgttaaa ttgtagggtc aagaagaaaa agcaggatag agtttatgta
    17881 aattgtcaat ccgatttaca gaaaagattt aaccacggat gaagatgcga aggcgatggt
    17941 cgcgaatact tggagcgaag ggtggaatgg acacagaacc cctacataaa aaacggacac
    18001 gattttaggc ttccaaaagg agactaaaat gagagaatat cccaaagaa
//
LOCUS       DAOQOS010000112        18013 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_1566_length_18013_cov_5.228088, whole genome shotgun sequence.
ACCESSION   DAOQOS010000112 DAOQOS010000000
VERSION     DAOQOS010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 18013)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18013)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18013
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1566_length_18013_cov_5.228088"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..479
                     /locus_tag="PLF66_19725"
     CDS             <1..479
                     /locus_tag="PLF66_19725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HMZ66331.1"
                     /translation="NANLEKQKIEEKLNYHEEKYRKLFECINEEVHLWEIIHDAFGEI
                     QTWRLVDANPTALNAWGKTREETIGKTTEHIWPDSNPVEQFMPIVKKIYIEKIPYTWE
                     SYFSGTNQTLLMTSVCFGEYFFSIGIDISKFKKQEKYFINQIKLKDKMIQEYKTKR"
     gene            complement(601..1065)
                     /locus_tag="PLF66_19730"
                     /pseudo
     CDS             complement(601..1065)
                     /locus_tag="PLF66_19730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020775557.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
     gene            1997..3277
                     /locus_tag="PLF66_19735"
     CDS             1997..3277
                     /locus_tag="PLF66_19735"
                     /inference="COORDINATES: protein motif:HMM:NF020037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HMZ66332.1"
                     /translation="MILPDIEFQKIFESVPALYLIVSPDLIIVAVSDSYNKATMTKRE
                     EIIGREIFEVFPDNPNDLKADGVSNLRASLDFVIRNKVPHTMAIQKYDIRNPEGEFEV
                     RYWSPLNTPVLNSDKEILYIIHRVEDVTEFIRMKDEKVKSDATIDELRLDLKEMEIEL
                     IQRSREIQKLNVELEQKVIDRTKNLEKANEKIQKNLHSLSFQKRQLEDFCNIISHNLR
                     SPLVNISMLIEFITESKVESEKKLLIEKLRIASNNLNEVFDQLVESLQIQEDTEIQSE
                     NLIIQDYLQKVLGGLQGQINKSQAVFQIDFNNAATVYYPPKYLTSILHNLISNSLKYH
                     SPDRGLIIRIQTESVANSIILSVSDNGLGIDLNKHKDNLFKIRKTFHSNPDAKGFGLF
                     ITKTQVEAMSGRIWAESMPNQGSVFYVEFKNQTK"
     gene            3274..3672
                     /locus_tag="PLF66_19740"
     CDS             3274..3672
                     /locus_tag="PLF66_19740"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HMZ66333.1"
                     /translation="MKSIKRIYLIDDDELFVYLLSRTIEATKLVENIEIFNNGQDAIE
                     FLKENTDKEEILPEIIFLDLSMPILDGWGFLDEYLFIKPKLNKKIELFILSSSVSPHD
                     VERAKKNLDVSDFIIKPMSKKGFIHIIEQM"
     gene            4207..4617
                     /locus_tag="PLF66_19745"
     CDS             4207..4617
                     /locus_tag="PLF66_19745"
                     /inference="COORDINATES: protein motif:HMM:NF016807.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BLUF domain-containing protein"
                     /protein_id="HMZ66334.1"
                     /translation="MFQLIYSSIESRAMDTDDLRDIVKKAVSANKAIDVTGLLLYVDG
                     FILQLLEGEKEIVLNLYQKIKKDLRHYDVETILEIERGKRMFPDWSMGFKSIHTNEIR
                     NFDGEVITSETLRSRCRIKPNLVFDLMATFIEKG"
     gene            complement(4814..6373)
                     /locus_tag="PLF66_19750"
     CDS             complement(4814..6373)
                     /locus_tag="PLF66_19750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004784375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HMZ66335.1"
                     /translation="MDKKKIPLDGIAGLRENWRNDIVSGFTIFLIALPLCIGIAIASG
                     APPMSGLFSGIIGGIIGSLLGGSYVTINGPAAGLIAVIISSINVLGGGDAKLGFELTL
                     AAIVIAGLLQIILGLLKAGNLNVYFPASVIHGMMAAIGIIIISKQVHILLGVSPKSKS
                     IIGLVAEIPNSILNLNPEISIIGVVGIVVLIVLPLINVSWVKKIPAPLAVVLIGMGIG
                     FYFNLEDQHTFTFLKKYYDIGPQSLVNLPAHIADGFASPNFSKILTLDFILMVITIVI
                     VGSVESLLTATAIDKIDPYRRTSNMNKELISKGVANFLLGMIGGIPIIAEVVRSSANV
                     NNGAKTRWSNFFHGVFLLVFIVLFPALIHEIPLAALAAILVMTGLKLASPIEFYHMYQ
                     KGLDRLFIFLVTIIFTLWEDLLVGVAIGIVLQIIVLLFKGLPFGSIFKADFTIESKDK
                     RHTIVVRKALVFSNYLSFKMAMFRLPLGEEVYIQFIDEPLIGLSVVEYLADFTLHYQE
                     SGGKVMVEGMQ"
     gene            6787..7293
                     /locus_tag="PLF66_19755"
     CDS             6787..7293
                     /locus_tag="PLF66_19755"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HMZ66336.1"
                     /translation="MSPMNYDRSPTGLYRVRFQDCDPFGHLNNARYIDYALDAREDQL
                     RRYYGFDTPSWTQSAGKGWVVSKMKANYLKSILWNEEIRISTKLVELGEGSLWVEAIF
                     SDPHSDKLKSLVWGNFVYIDIKSGRPVRHESDLKEFLNSILYTKEWEGKEDIDRRVQS
                     LRRNLVAV"
     gene            7335..7874
                     /locus_tag="PLF66_19760"
     CDS             7335..7874
                     /locus_tag="PLF66_19760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HMZ66337.1"
                     /translation="MATKRKSKATTLKKSNTTEVKDEILSLPNDFVREMRKSGVRCLH
                     LNLRRAVRTVDRHFDFALEKVGLTANRFNILMTLGANPMGMELAPLAQLLVYDRSTLL
                     RNLEPLEESNWIRDIPSDTKRARKIALTQAGIRKLKDGLVAWNAAQSKSELVLGGSDY
                     SLFLKKLRILANQENLIND"
     gene            8191..9054
                     /locus_tag="PLF66_19765"
     CDS             8191..9054
                     /locus_tag="PLF66_19765"
                     /inference="COORDINATES: protein motif:HMM:NF025028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY domain-containing protein"
                     /protein_id="HMZ66338.1"
                     /translation="MTLITMEKAWGIKAKERSSCGRCWFKMDEEPFPLRSVYNGTYSL
                     FGYIKSSNGAILIYPNFLIDVPLSFALDTVLLPISIPNAIIREMNFRGYSVSTDKFSI
                     RKVYFQDEELDLNPDDFIILKNGNDQYARDSKKVYYRKYQIEGADPDSFNVLEEKEKF
                     YSKDKNRVFFEGTEIKGADVISFQAINAHYSKDKKSVFFKREIIDGANPIKFRFFQIS
                     SEAKLGASASCDKVPSELYSTDGQFVFYGATKIKDADIKTFVVTGKGCNTAEDKYHFF
                     LGDSSYKKEIK"
     gene            complement(9144..10589)
                     /locus_tag="PLF66_19770"
     CDS             complement(9144..10589)
                     /locus_tag="PLF66_19770"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HMZ66339.1"
                     /translation="MDNSVFNKILRERELKNEKSVALIRLLFVTITILSDLAAYFGLI
                     HFNAIQPDNTTILLDTFFLFFSIIVFLFILRKEYSRYLKFFTISCDYLIISMMLALDP
                     TISREGEFFILAQAFAALYIYFINLLRYSKAGTIYSAFLAVVMFAVMNYFFNVNHQYM
                     IIPMSLALFMMLYIGYSLTNSSVQMMIEANTKKMMERYLPPELVGELYKTNASLEPGG
                     NNQVVTILFSDIRSFTSISESLSPQIVVQMLNDYLSTMTDIIFANRGTIDKFIGDAIM
                     TIFGAPVKESDDAFRAVNTALEMIQSMKAFNQKYSHLGKKLEIGIGIHTGDVIVGNIG
                     SEKRLDYTVIGDNVNLSSRIEGLTKHYKCPILISESTFQELKNNNSEKSFCIRLVDDV
                     IVKGKTVSIKIYEVFHVESYSEREEKNRLKEIFESGLEYYRSQKFREAAEIFKSLPND
                     EVSKMYLNRIQKLIETPPDQSWNGKYKLDEK"
     gene            10887..12413
                     /locus_tag="PLF66_19775"
     CDS             10887..12413
                     /locus_tag="PLF66_19775"
                     /inference="COORDINATES: protein motif:HMM:TIGR04452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04452 family lipoprotein"
                     /protein_id="HMZ66340.1"
                     /translation="MKEILGIIMISMGLSFCNAFPGIDTISGLEAKKKIKTAANSIDR
                     LSLGIVSSTSASASIVGMINNMLVPAMISISSTKYYDKASVNDCVKKIISITGLILGG
                     ATTLPLCDIQEASFIDIGPIDIIDGKKADVNDNLAFFLLFGSSDSLTLPGLGTGAGTS
                     SGSSGTSGGTSSTPAACTTSGATDGTIQQLSFSVVDAEYDLTNNRIVLVDSANKFHYY
                     NPSTNADTSVSLNYSPLNVTVSPDGTKAAVGHNANATLINLPIATIAREVATSMTAND
                     IAMSNTYVYVSNAFAANPYNVRSITFSDGSIVNHSGSSTYPSFLAYNSAGNSLYLIDY
                     GLSPTDIRKLNTAVNPPTYLYDSIYHGDYLMCGSIWLAKDSSKTFNSCRTVFTVSATQ
                     GSDIQYIGTLTSTSLLRITHLNTDTSKVFLIPSNKLFSNSTPDTTKDSSEYIHTYNIS
                     NLSFISKKTLPCYTTGVISYKTEGKFIFVNSSSTEYYVIAKPDSTSGNQNDQLIKFPY
                     "
     gene            complement(12944..13936)
                     /locus_tag="PLF66_19780"
     CDS             complement(12944..13936)
                     /locus_tag="PLF66_19780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66341.1"
                     /translation="MSKLPFYYKYCIILLIILCMNCLTAISRAKFSQKIERKSTYPER
                     LIHIQESKNFLKFIWEELQTMQIETDFGHSNKISESKILRTACIEVPTDTKNNIIEVL
                     NYDHCPNNEIFTNANQQAKPELNKFIYFPLNKIFYKTPVDQYVRESSYLNNPEIESIE
                     SLFDSKVLFNKQINRIYIKQKNGNILKFEPRSVWKNNKYFRSYISVERQNSDRYWIKD
                     SVELPKVQTKDSLLTVLIFKSYKPNQTTGSFDLFTEIILLDLKNENWKNKTITFANYE
                     IERKTKFGIYPLLIPLTIPVDILTAPIQLAFLGFNHYVYIISCSLGFTCKTALG"
     gene            14500..15087
                     /locus_tag="PLF66_19785"
     CDS             14500..15087
                     /locus_tag="PLF66_19785"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HMZ66342.1"
                     /translation="MNYLLIYAHSNPKSFTNAVYQSVYAELSLKGASIQVIDLYKDKF
                     NPILYVDENHRRRDLFKDPETLIYRNKIERADHLIFIYPVWWHGFPAILKGFIDRVIS
                     SEFAYSFKNKNKDALFPEGLLSDKKISCFYTLDAPSLVAFLDPGWLSIKYTIFKYCGF
                     KQVERYYLSNLKHLDDKKRREWLLKCRKIVRTFKN"
     gene            complement(15268..16824)
                     /gene="lysS"
                     /locus_tag="PLF66_19790"
     CDS             complement(15268..16824)
                     /gene="lysS"
                     /locus_tag="PLF66_19790"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002973913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HMZ66343.1"
                     /translation="MSEHELEQNELIKQRIEKIEELKQKGINPYPIRFFPDSYSVDLI
                     KNYTLIMNENETLQKMEKEKNPNADTSKIHILHKLAGRIHSKRIMGKASFAHLKDRDG
                     IIQMYAAEKDLGEENYHLFKTLDLGDHIGLEGYLFTTKTGEISIHITSFQLLAKCIRP
                     LPVAKEKDGVVFDAFSDKEQRYRMRYVDLVVNDSVRETFIMRSKIIAEIRSFLIKDGF
                     LEVETPMMQPIAGGAAAKPFVTHHNALDMQLYLRIAPELYLKRLIVGGMDRVFELNRN
                     FRNEGISIKHNPEFTMMEAYMAYGDMETMLKLCERMIVHVATAIGKGLKFKYGEDNID
                     LTPPWKRRKYVDIIQDFSGIDFSKIKTLDEAKAEAKKAGVKADDATSIWKVADEVFSE
                     KVEPNLIQPVFVTDFPKELSPLAKSREDDPNYVERFEPYIVGREIGNAFSELNDPFDQ
                     KARFEEQVKMREAGDEEAFMMDNDYIRALEYGMPPTGGLGIGIDRLVMLLTDSASIRD
                     TIYFPLMRPE"
     gene            17024..17479
                     /locus_tag="PLF66_19795"
     CDS             17024..17479
                     /locus_tag="PLF66_19795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66344.1"
                     /translation="MKKFILYCLVITVLPVFSIEKIEPDPKALKLVSDFMNALTIADE
                     AERLQAILPLVHDSLKTKDKKDLFSSVKQFSYKRAVDMIKLYKAPAEVSEVHKGAVST
                     IGYKELAEKGRRDKYFVKKKEGVNGMPAPIHVFIPENGGEPKIYDFGSL"
     gene            17523..17939
                     /locus_tag="PLF66_19800"
     CDS             17523..17939
                     /locus_tag="PLF66_19800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004788023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HMZ66345.1"
                     /translation="MNIELKRIQEPFLFEAKNATGNSIHIDASPQIGGTDKGVRPMEL
                     LLMGLAGCVSIDVINILRKQRIAVLDYNVNVEGERETGKDANLFTEITVHFFVKADVE
                     EDKLIRAIELSLDKYCSVAKTLEKTAKIKYTFRIER"
BASE COUNT         6267 a         2844 c         2918 g         5984 t
ORIGIN      
        1 ttaatgccaa tttagaaaaa caaaaaatag aagagaaatt aaattatcat gaagagaaat
       61 acagaaaact ttttgaatgc atcaatgaag aagttcatct ctgggaaatt atccatgatg
      121 catttggaga aattcagacc tggagactag tagatgcaaa tcccaccgca ctcaacgcat
      181 ggggaaaaac aagagaggaa acaatcggaa aaactaccga acatatctgg cctgactcaa
      241 atccagtcga acagtttatg cctatcgtta aaaaaattta tatagaaaag attccctaca
      301 cttgggaatc ttattttagt ggaaccaatc aaactctact tatgaccagt gtatgctttg
      361 gtgaatactt cttttccatt ggtattgata tttctaaatt taaaaagcaa gaaaaatatt
      421 ttatcaacca aatcaaactc aaagataaaa tgattcaaga atataaaact aaacgttaat
      481 aggtatagac tgaaatacga ttcagttcta gaatttttgc tttaaaaaaa gctttgttta
      541 tcctgttgat tatcggatac taaaaaaatc tctattaaaa aaactagacc catcacagga
      601 tcagccaaac tttttccaga atttatgatt gggagttcta ctgtccccgt ttttagaaaa
      661 ttcttttttg agtccgttta cataatacat ttctaattcg cttttatttt tggcgtttac
      721 ctttccacgg tattcgcaat caaaaaaatc ttttactaag ttgtaagtga ttccgctaat
      781 attgatttta ggaattcccc ctgcacacat ataagaatcc ccaattgttt tgagtttttc
      841 taaattgtat ctctcaataa ttttatcaaa ttgaacaaag caagcatcta gatccttgat
      901 gagatcttgt gggcttagct tctctgcaat ctgagtaaat cctttgaagt ctgtaaacat
      961 aacacttact tgttcaaaaa aagtgggttc tgcaaaacct ttttctttca gttctgttgc
     1021 tacatctttg ggtaaaatat ttaacaaaag cttttcagac ttttgacttt caacctcaac
     1081 tttttctaat aaagaaaagt tttgaattac tcctgcaact tgaccacaga agatggaaat
     1141 tttttcttga tcttttttat ttagtcttaa tttttctttt ccatgataga agtctaaggt
     1201 tccaatcaat ttatctttaa caaataaagg aacaataaaa agagaatggt gatttagtaa
     1261 aatttggttc ttaacttcac cttcatcatt cgaatttttt ttttaacagt atctggaacg
     1321 tactgcaatc ttaaacttgt acagtatgat aaatatctaa aggtaaatct tttagagcaa
     1381 gaaaaaacag tttaactcct gcttttagag tttattgacg tatatctttt agattttatt
     1441 aaaactctct atagaaaata aattaaagtg agttatggta ggcttaggat ttcgagactc
     1501 ttaatatcca aactctttct agttcaaaac aactaatttc gaaaaatccg caatcctttt
     1561 tcgcttaaag acttaatgca aagactaaag atactcaatc ttagttcaaa ttaaattact
     1621 tcttttccag aatcgatagg tgatctaaag aaaataacaa tgttttaact caagcacaat
     1681 ccttttttca agcaagaaca agaaagaata aaaaaaatat gcaaaatttt aggatctatt
     1741 gggattcgtt ttactttgat ctaatttttc tggtaagtat cttgctatat aaaggtttct
     1801 tctaacacgg ataatcttat ttatcctatt ttatttttaa tctttattcg gtaagacaat
     1861 actattgata ttgcataact agagtaaatt acttgacatg acctagattt tattttgaaa
     1921 tatggtaata cctagactaa caataatttg tcactaacca ttcggaatga cgttggtata
     1981 aatggggaga gcaaaaatga ttttaccgga tattgaattc caaaaaattt ttgaatcagt
     2041 gcctgcgcta tacctaatcg tttctcctga tttaattatt gttgctgtta gcgattcata
     2101 caataaagca actatgacca agagagagga aattattggg agagaaattt tcgaagtatt
     2161 tcctgacaat ccaaacgact taaaggcgga tggcgtttcc aatctccgcg cttctttaga
     2221 ttttgtcata aggaataaag ttcctcacac aatggcaatt caaaaatatg atattcgtaa
     2281 tcctgaagga gagtttgaag tcagatattg gagtcctttg aatactcctg tcttgaattc
     2341 tgataaagaa attttatata ttatccaccg agtggaggat gtgactgagt ttattagaat
     2401 gaaagatgaa aaagttaaga gtgatgctac tattgatgaa ttgcggttgg atttgaaaga
     2461 gatggaaata gaattgattc aacgttcacg cgaaattcaa aaattaaatg tagagcttga
     2521 acagaaagta atagatagaa caaaaaactt agaaaaagca aatgagaaaa ttcaaaagaa
     2581 tttacattcc ctttcttttc aaaagcgtca gttagaagat ttttgtaata ttatatcgca
     2641 taacttacgt tctcctctcg ttaatatttc tatgctaatt gaatttataa cagagagtaa
     2701 agtggaatct gaaaagaaat tattaattga aaaattaaga atagcatcta ataatttgaa
     2761 tgaagttttt gatcaattag tagagtcact tcagattcag gaagatacgg aaatacaatc
     2821 cgaaaactta atcattcaag actatctaca aaaagtattg ggtggattgc aaggacagat
     2881 taataaatct caagccgttt ttcagataga ttttaacaat gcggcaacag tttattatcc
     2941 accaaagtat ttaacgagta ttttacacaa tcttattagc aattcattaa aatatcactc
     3001 tccagaccga ggcttaatta taagaataca aacagagagt gtagctaata gtattatact
     3061 atctgtttca gataatggct tgggtattga tttgaataaa cataaagata atttatttaa
     3121 gatcaggaaa acttttcatt ccaatccaga tgccaaaggt tttggattat ttattaccaa
     3181 gacacaggtg gaagccatga gtggacgaat ttgggcggag agtatgccaa atcaaggctc
     3241 agttttttat gttgaattta agaatcaaac taaatgaaaa gtataaaaag aatttatctt
     3301 atagacgatg acgaactttt cgtttactta ctcagtagga cgattgaggc aacgaaactg
     3361 gttgaaaata ttgaaatatt taataatggt caagatgcaa ttgaattctt aaaagagaat
     3421 acggacaaag aagaaatctt accagaaata atttttctag atctttcaat gccgatacta
     3481 gacggttggg gatttttaga tgaatatcta tttataaaac ctaaattaaa taaaaaaatt
     3541 gaattattca ttttatcatc ttcggtttct ccgcatgacg ttgaacgagc aaaaaaaaat
     3601 ttagatgtct cagactttat aatcaaaccg atgagtaaaa aaggattcat acatattata
     3661 gaacaaatgt aaatttctac ctaagtcgca tatcatcacg tctataacga gtcaattgct
     3721 cttactgatt ctctcattct ttatttgtaa atagctttaa cttgtcttat cttataatcc
     3781 ttataaaaaa gtattaacaa ataaaatttg caaccattag aacaaaatat cggattctag
     3841 aagaactgtg aataggagac acggatattt taaagataaa ttcatctatg ataccatcag
     3901 ataaatactt caagtaagaa tatggggttc ttttgggcaa acagatgtta tccgttactc
     3961 gcaatattat cttatcaaat aggaaaaatt agatattgga ctttttctaa gagaaataat
     4021 tttaggaacg atcggttctg attctcaact tgatacgaat tgcgttggcg atagagttta
     4081 taatcttgaa actagagaga ataatagagt gatttcagat aagtacatcc tcactctata
     4141 gagtagagaa attttattga cgttattctt ttttcatcaa gaatgtaagg actgttagga
     4201 gtaaatatgt ttcaattaat ctattctagt atcgaatcta gggctatgga tacagatgat
     4261 ttacgagata ttgtgaaaaa agcagtttct gcaaataaag cgatagatgt aacaggatta
     4321 ttactatatg tagatggatt tattttgcaa ttattagaag gggaaaaaga aatagtctta
     4381 aatttatatc aaaaaataaa aaaagatcta aggcattatg acgtagaaac catattagaa
     4441 atagagagag gaaaaagaat gtttcctgat tggtcgatgg gatttaaaag tattcacacg
     4501 aatgaaatta gaaattttga cggtgaagtg attacctcag aaactctaag gagtagatgt
     4561 cggataaagc ctaatttagt ttttgattta atggctactt tcattgaaaa aggataaagg
     4621 catagattaa aaaataatct gttgtttata aaaaatcctg caagctttca aaaaaaataa
     4681 gttaaataaa ttgcaattct agagaagaaa ataaatgaaa atttaaacca tcgaaaactg
     4741 atattcagtt ctcctgcatt tttgctaaat gaaagtctat ctggtaattt tctattcgct
     4801 ttttaagaaa catttattgc atgccttcta ccattacctt tccgcctgac tcttgatagt
     4861 gcaaagtaaa atccgctaaa tattcaacaa cggatagtcc aatcagaggt tcatctataa
     4921 attgaatata tacttcttcg cctaatggta acctaaacat tgccatttta aaggacaaat
     4981 aattcgaaaa tactaatgct ttacgcacaa cgatcgtatg acgtttatct ttggattcta
     5041 ttgtaaaatc tgctttaaaa atactaccga aaggaagtcc tttaaataat agaacaatga
     5101 tttgcaatac tatgccaatc gctacaccta caagcaaatc ctcccaaaga gtaaaaataa
     5161 ttgttaccag aaaaataaat aatcgatcaa gacccttctg atacatgtga taaaactcta
     5221 taggagatgc taattttaag cctgtcatca ctaagatagc agctaacgca gcaagtggaa
     5281 tttcatgaat cagtgctgga aataaaacta taaaaactaa caaaaatact ccatgaaaaa
     5341 aatttgacca acgtgtcttt gctccgttat taacatttgc agaacttctt actacctctg
     5401 caataatcgg aatcccacca atcattccta aaagaaaatt ggctacacct tttgaaatta
     5461 attctttgtt catattgcta gttcttctat aggggtcaat cttatcaatt gcagtcgcgg
     5521 ttaataatga ttctacactt ccaacaatca caattgtaat caccattaaa ataaaatcaa
     5581 gagtcaagat tttagaaaaa ttaggggagg caaacccatc ggctatatgt gcaggaaggt
     5641 ttactaaact ttgtggacct atatcataat atttttttaa aaatgtaaat gtatgctgat
     5701 cttctaaatt aaaataaaat ccaatcccca ttccgattaa tacaacagct aaaggagcag
     5761 gtattttttt tacccatgaa acattgataa ggggtaatac tatcagaacg acaatgccaa
     5821 caacacctat aatagaaatt tcaggattca gatttaggat actattagga atttcagcga
     5881 ccaagccaat gattgatttt gatttgggac taacgcctaa tagaatatga acttgctttg
     5941 aaatgataat aattcctatc gcggccatca tcccatgaat aacggatgcc ggaaaatata
     6001 catttaggtt accagctttt aacaaaccaa ggataatttg aagtagacct gcaataacaa
     6061 tagcagctaa cgttaattcg aaacccaatt tagcatcacc accgccaaga acgttgattg
     6121 aacttataat tacagcgatc aaaccggcgg caggaccatt gattgtaacg taagaacctc
     6181 cgagcaatga gcctataatt cctccaataa ttcctgaaaa taatccagac attggtggtg
     6241 caccagaggc aatagctatt ccaatacaaa gcggtaacgc gattaaaaaa attgtaaatc
     6301 cagaaactat atcgttgcgc caattttctc ttaaaccggc tatcccatcg agtggtattt
     6361 tcttcttgtc cataatctgc ctttaaaata ggttcaatag gagatatcaa atttacagct
     6421 atatttataa aatatagcaa gtgatacaga acacaaatta tacttaaccg caaggatata
     6481 gtcagagtag caaaggatag aaactcatta ttagtatgaa caatatgtat tgattcgaag
     6541 cgttatgtgt tagtattaaa attttacccc atctagatca aatcatcttt ttataaaaga
     6601 ctcgcaatct ttttttaatt tcaggtttca tttttaaaaa ggaatctaaa actattgtcg
     6661 aaaaagttag atgaaaagtg tctgctatat gccattaaga aattgaactt gtttaaacag
     6721 aaaaaattgg tttgacaatt atctatgtaa atacatatat gtagttacat agataaagag
     6781 gattttatga gcccaatgaa ttatgacaga tctcctaccg gattgtatcg tgttcgattt
     6841 caagattgcg atcccttcgg acatttaaac aatgctcgct atatagatta cgctctcgat
     6901 gcacgagagg atcaattacg acgttattat ggttttgata ccccttcttg gactcaatct
     6961 gctggaaaag ggtgggtggt ctctaaaatg aaagcaaact atctaaagtc tattttgtgg
     7021 aatgaggaaa ttcgaatttc cacgaagctt gttgaattgg gggaagggtc tttgtgggtg
     7081 gaggctattt tttcggaccc acattctgat aagttgaaat cacttgtatg gggtaatttc
     7141 gtttatattg atataaaatc gggtcgtccc gtacgacatg aaagtgattt gaaagagttc
     7201 ctaaattcaa ttctctatac taaagaatgg gaaggcaaag aagatatcga tcgtagagtg
     7261 cagtccctca gaagaaatct agtagcagta taaaaactgc tcgccatata tttgatattc
     7321 tttaaaattt ttctatggct acaaaaagaa aatcaaaagc tactactttg aaaaagagta
     7381 atacaactga agtgaaggat gaaatacttt ctcttccgaa tgactttgtc agagaaatgc
     7441 gaaaaagcgg agttcgatgt ttgcatttaa atcttaggcg tgcagttcga acggtagacc
     7501 gccattttga ttttgctcta gaaaaggtag gacttactgc gaatcgattt aatattttaa
     7561 tgacgttagg tgcgaatccc atgggaatgg aactggcacc tcttgcacaa ctacttgtct
     7621 acgaccgttc tacactcctt agaaatttag agccactaga ggagtcaaac tggattcgtg
     7681 atattccttc agatacaaaa cgagcccgta agattgctct cactcaagct ggaatcagaa
     7741 aattaaaaga tggattagta gcctggaatg cagcccaatc aaaatcagaa ttagttttag
     7801 gtggaagtga ttattccctt ttcttaaaaa aactaagaat acttgcaaac caagagaatt
     7861 taataaacga ctaatttatt ttattctaaa actctctcgc tataaagctg aaaatatttt
     7921 ttaaagactg attcgaaaat attatttgct ctaaggcatt catttacaag attgaatggg
     7981 tgataatttt ggataaaagg attcaagtta gccattagcc gctagaaaga aatatttatg
     8041 atcaattcag aaaattgtat tgactagctt cctgttgcaa gaattaaaaa gattgaggta
     8101 attttgaagc catatctagg aaaagaaaat tttttacaga cagtattttt gcttatcagt
     8161 ttttgtattt tgattatttc aaataactgt atgacattga taactatgga aaaagcatgg
     8221 ggcataaaag caaaggaaag atctagttgt ggaagatgct ggtttaaaat ggatgaggaa
     8281 ccatttccgt tacgctcagt ttacaatgga acttatagtt tgtttggtta tatcaaaagt
     8341 tcgaatgggg ctattttaat ttatccaaat tttctaatcg atgttccact cagttttgct
     8401 ttggatacag ttttacttcc aatttcgatt cctaatgcaa ttattagaga aatgaatttt
     8461 cgcggttact cagttagcac agataaattt tcaattagaa aagtatactt tcaggatgag
     8521 gaattagatt tgaatccaga tgattttatt attctaaaaa atggaaatga tcaatatgct
     8581 agagatagta aaaaagtcta ttatagaaaa tatcagatcg agggcgcaga tccagatagt
     8641 tttaatgtat tagaggaaaa agaaaaattc tattccaaag ataaaaatag agtgtttttc
     8701 gaaggtacag aaatcaaagg tgcagatgta atatcatttc aagcaattaa tgctcattac
     8761 tccaaagata aaaaatccgt tttttttaaa cgagaaataa tcgatggtgc aaatccaatc
     8821 aagtttagat tttttcaaat ttcttcagaa gcaaaattag gggcaagtgc ctcctgtgat
     8881 aaggtaccct ctgaattata ttctacagat ggccaatttg ttttttatgg ggcaactaaa
     8941 ataaaagacg ctgatatcaa aacttttgtt gtaacgggta aaggatgtaa tactgcggaa
     9001 gacaagtatc atttcttttt gggagattct tcctataaaa aagaaattaa gtaactgtat
     9061 aaaatgataa taagaaacaa atatagccgt ttcaaaaaaa attatcaaac agcttataga
     9121 tataattgga tttagagaat aatttatttt tcatctaact tgtattttcc gttccaagac
     9181 tgatccggtg gtgtttcgat taacttttga attcgattca aatacatttt ggaaacttca
     9241 tcatttggta aacttttaaa tatttcggcg gcttctctaa atttttgact ccgatagtat
     9301 tccaagcctg attcaaaaat ttcttttaag cgattctttt cttctctttc cgaataggat
     9361 tccacatgga atacttcgta aattttaatg ctaacagtct ttccttttac aatcacatca
     9421 tccactaggc gaatacaaaa cgatttctca gaattattat ttttcaattc ctgaaaagtt
     9481 gattcagaaa ttaaaatagg acatttataa tgtttagtta aaccttctat tcgtgaggat
     9541 aaatttacat tatccccaat gactgtataa tccaatcgtt tctctgatcc aatatttcca
     9601 acaatcacat cacctgtgtg aattccaatt ccaatctcaa gtttttttcc taagtgagaa
     9661 tacttttgat taaacgcttt catagactga atcatttcca atgcagtatt tactgctcgg
     9721 aatgcatcgt ctgattcttt tactggagct ccaaagattg tcatgattgc gtctcctata
     9781 aatttatcaa tcgttcccct gtttgcaaaa attatatctg tcattgtaga aagataatcg
     9841 tttagcattt gcacaactat ttgtggagat aaagattcag aaatggaagt aaaggaacga
     9901 atgtcagaaa ataaaatagt tactacttga ttgtttccac cgggctctaa actcgcattg
     9961 gttttgtata attctccgac tagttcaggc ggcaaataac gttccatcat tttctttgtg
    10021 ttagcctcaa tcatcatctg aacactagaa tttgtgagtg aatatccgat atatagcatc
    10081 atgaaaagag ccaaactcat tggaataatc atatattgat gattcacatt aaaaaagtaa
    10141 ttcatgactg caaacatgac aactgctaaa aaagcagaat aaatagttcc cgctttggaa
    10201 taacgaagta gatttatgaa atagatatac aaagcagcaa aggcttgagc caagataaaa
    10261 aattcacctt ctctcgaaat agttggatca agtgccaaca tcatagaaat aattaaatag
    10321 tcgcaactga tagtaaaaaa tttcaaatac cttgaatatt cttttcttaa aataaagaga
    10381 aaaacaataa ttgaaaaaaa taaaaaaaat gtatcgagga gaatcgttgt attatccggt
    10441 tggattgcat taaaatgaat caatccaaaa tatgcggcta aatcggaaag aattgtaatt
    10501 gtaacaaata ataatcgaat cagcgcaaca gacttttcgt tttttaattc tctttctctt
    10561 aatattttat tgaatactga gttatccata gagtttttac ttggcacctt ctatcttcgt
    10621 tagcttccag aattgtttct ttccattcca tctgtgttat taaaaaataa tagaaatttt
    10681 aaaatttccc acatctagtt caaaacatat gattctaaaa gacccgtaat cctgtatcgc
    10741 ttaagtgatt tacttgcaaa ataaaatagg ggactattct ctaaaaagca gatagaaatt
    10801 actagaatag gaatagaagt tacagtattt ttatacatgc ctaaatataa cttgatagtt
    10861 ctttctaaat tctttaatct ggttatatga aagaaatatt agggataata atgatctcaa
    10921 tgggtctctc tttttgcaat gcttttccag gaattgatac tataagtgga ttagaagcaa
    10981 aaaagaaaat taaaactgct gcaaacagca tagatcgatt atcgctcggt attgtgagca
    11041 gtacttctgc atccgcatcg attgtaggaa tgataaataa tatgttagtg cctgcaatga
    11101 tcagtattag cagtacaaag tattatgata aagcatcggt caatgattgt gttaaaaaga
    11161 taatttcaat tacaggttta atacttggtg gtgcaactac acttcctctt tgcgatatcc
    11221 aggaagcaag tttcatcgat ataggtccaa tcgatattat tgacggtaaa aaagcagatg
    11281 taaacgataa tctggcattc tttttacttt ttggaagttc cgatagtctg acacttcctg
    11341 gacttggaac tggagcagga acaagttctg gatcctctgg aacaagtggg ggaactagtt
    11401 ctactccagc tgcatgtact acatctggcg cgactgatgg tacgattcag caactttcct
    11461 tttctgttgt agatgctgag tatgacctaa caaataatag aatcgtactt gtagactccg
    11521 caaataaatt tcattattac aacccatcta caaatgcaga tacttcagtg tccttaaatt
    11581 attctccttt gaatgtgact gtaagccctg atggaacgaa agcggcggtc ggacataatg
    11641 caaatgcaac tttgatcaat ttaccaatag ctacgattgc tcgagaagtt gcgactagta
    11701 tgactgcaaa tgatattgct atgagcaata cttatgtata tgttagcaat gcgttcgcag
    11761 cgaatcctta taatgtgagg agtatcactt tttctgatgg atctattgtg aatcattcag
    11821 gaagttctac ttatccttct tttttggcat ataactccgc aggcaattct ctttatttaa
    11881 tagactatgg tctttcccca acagacattc gaaaattaaa tacagcagta aatcctccca
    11941 cttatctcta cgattcaata tatcatggag attatttgat gtgtggatct atttggcttg
    12001 caaaagattc tagcaagact ttcaattcct gtcgtactgt gttcacggtt tctgctacac
    12061 agggtagtga tatacaatac attggaactt tgacttcaac ttcccttctt cgtataacgc
    12121 atttgaatac agatacttct aaagtttttt tgattccctc aaataaacta ttcagcaatt
    12181 caactcccga taccacgaaa gattctagtg aatatattca tacctataat atttcgaatc
    12241 taagttttat ttctaagaaa acattgcctt gttatacgac aggtgttata tcctataaga
    12301 cagaaggtaa atttattttt gtaaatagtt ctagcacaga atattatgta attgcaaaac
    12361 cagattctac ttctggaaat caaaatgacc aactgataaa attcccgtat taatcaataa
    12421 accttgttgc tatctagcaa caaggcaaac ttactcctca aaataaaatg atttttattc
    12481 gaaagaaaaa cccctacgga taaggcttga atctagaaaa aaatttcaga gtaaaatatt
    12541 tctcctttat tttaaaaaca aatttattag aagagattca tcccatctat ttttgcttta
    12601 agcttttaag ttgtaggata gaataaggtt gaatgtgaaa aaattataga aggaaattag
    12661 atcatagttg ttagaaataa atctaaacta acctttatga attatctaaa tgagtaataa
    12721 aattaaaaat ttataaaaat gtatctttga ttatagcaag taggtatatt catataatcc
    12781 tcacaataat gaaaagaatt tcttttcaac atttcaaaac tctcaaaaaa caataaaaat
    12841 cttagcattt tatgagtaaa aatagatcag ataaaacgtg agtcgcaatt ttaatggggt
    12901 ttgaaaatag atcaaaatga taagtttgtg cgttatgcaa aaatcaacct aacgcagttt
    12961 tacatgtaaa acctaaacta caggatatta tataaacata atgattaaaa ccgagaaaag
    13021 ctaattgaat aggtgcagtt agaatatcta ccggaattgt gaggggaatt aacaatggat
    13081 atattccaaa tttagttttt ctttcgattt catagttagc aaatgtaatt gttttgtttt
    13141 tccaattttc atttttcaaa tcgagtaaaa ttatttcagt aaacaaatca aaactacctg
    13201 ttgtttggtt tggtttatag gatttaaaaa ttagcacagt aagaagcgaa tctttagttt
    13261 gaacctttgg aagttcgaca gaatctttaa tccaatatct atcactattt tgccgttcta
    13321 ccgagatata actcctaaaa tatttattgt ttttccatac agatctaggt tcaaatttta
    13381 atatatttcc atttttttgt ttaatgtaaa ttctatttat ttgtttattg aataaaacct
    13441 tagaatcgaa taaagattct attgattcaa tctctggatt atttaagtag gaactttctc
    13501 tgacatactg atcaacagga gtcttataaa aaattttatt taaaggaaaa tatatgaatt
    13561 tattcaattc cggttttgct tgctgattcg catttgtaaa gatttcattg ttcggacaat
    13621 gatcataatt taaaacttct attatattat tcttagtatc agttggaact tctatacatg
    13681 cagtccttag aattttcgat tcggaaattt tattagaatg accaaaatct gtttctatct
    13741 gcatggtttg aagttcttcc catataaatt ttaaaaaatt ttttgattcc tgaatatgaa
    13801 tcagtctttc tggataggtt gattttcttt caatcttttg ggaaaatttt gctctgctta
    13861 ttgctgttaa gcaattcata cagagtatta tcagtaaaat tatacaatat ttataataaa
    13921 aaggaagctt tgacattatt caaatgattt cttcttaaaa aattatttca actttttatt
    13981 tagtatacaa tagtagacat ttaaattcaa tatttcaatg atcacaatcc aattcattct
    14041 ggatgatgta ttgagtatat atttcattta cttgaaatag aattagttct atcttccaat
    14101 ttcatcactc ggaaatttag atgttctttc tagaagtaac ctttttgact aactcttaat
    14161 gcctctctcg tcaattctgg agtaaatgca gattatcccc aattattatg atttggagcc
    14221 ctatgaagat gaaggatggc tttcacccat tctttagaaa gttcttgttt tagatgcaaa
    14281 gaattccagt tttttgaatc aatggctacc cacctgcggc attaggtaat aatgcttgaa
    14341 gttaagtaaa aaaatacttt ttaaaaaagt cttggaatag aaaatgaatg aaaactctat
    14401 gtctaagggc gaataacacc cattatgcta tatgctaaac aaaaaccttg tgtatcaaaa
    14461 ggaataaatt catagaatct agaatctcaa ggaatcatta tgaactattt actaatttac
    14521 gcgcattcaa atcctaagag ttttacgaat gcagtctacc aatctgtata tgctgagttg
    14581 tcgttaaaag gagcaagcat tcaagtaatc gatttgtata aagataaatt taatcctatt
    14641 ttatatgtag atgaaaatca caggagaaga gatcttttta aagatcctga aactctaatt
    14701 tatcgaaata agattgagag agctgatcat ctaattttta tttatcctgt ttggtggcat
    14761 ggtttccctg ctattttaaa gggatttatt gatcgcgtga tttcttctga gtttgcctat
    14821 tcatttaaga ataaaaacaa agacgcatta tttccagagg ggttactcag tgataaaaaa
    14881 atatcttgct tttatacgtt agacgctcct tcactagttg cgtttttgga tccaggttgg
    14941 ctttctataa agtatacgat ttttaaatat tgcggattta agcaagtaga aagatattat
    15001 ttgtctaact taaaacattt ggatgataag aaacgacgtg agtggctact taaatgcaga
    15061 aaaatagtta gaacttttaa gaattgaact gcaagagatg tgtatgcctt ctttctctgc
    15121 tagcccatgc acgaaaacag gttcttttta aaaaaatcta ggaaagaaaa atctactctt
    15181 tttagatcaa aacaattttt tcaaaaaacc ctacaagctt ttcgtgctat gagtctctgc
    15241 tatggagaat ttttcagaaa gaaactttta ttccggacgc atgagtggaa agtaaattgt
    15301 atcacgaata gaagcagagt ctgtgagtag cattaccagt ctatcaattc caattcctag
    15361 tccacctgtt ggaggcattc catattcgag tgcacgaata tagtcattgt ccatcatgaa
    15421 tgcttcttca tcaccagctt ccctcatttt gacttgttcc tcgaatctag ctttttgatc
    15481 aaatggatca tttaattcgg agaaagcatt tccaatttct cttccaacaa tataaggttc
    15541 aaatctttct acataatttg gatcatcttc tcttgatttt gcgagaggtg ataattcttt
    15601 tggaaaatct gtaacaaaaa ctggttgaat taaatttggt tctacttttt cagagaatac
    15661 ttcatctgct actttccaga tagaagtagc gtcatccgct ttgactccag ctttttttgc
    15721 ttcagctttt gcttcatcta aagttttgat ttttgaaaaa tcaattccag aaaagtcttg
    15781 aatgatatct acatattttc ttcgtttcca cggcggggtt aaatcaatat tatcctcacc
    15841 gtatttgaat tttagaccct taccgatagc tgttgctaca tgcacaatca ttctttcaca
    15901 tagttttaac atggtctcca tgtccccgta agccatatat gcttccatca ttgtaaattc
    15961 gggattgtgc ttgatagaaa ttccttcgtt tctaaaattt cgattgagct cgaaaactcg
    16021 atccatgcca ccgacgatca gtctctttaa ataaagttcg ggtgctattc ttaaatacaa
    16081 ttgcatatct aaggcattgt ggtgggtaac aaatggtttt gctgcggcac ctcctgcaat
    16141 tggctgcatc ataggtgtct ccacttctaa aaagccatct ttgattaaga agctacgaat
    16201 ctctgcgata attttacttc gcatgataaa tgtttctcta acagagtcgt tgactactaa
    16261 atctacataa cgcattctat atctttgttc tttgtcacta aatgcatcaa aaacaactcc
    16321 atctttttcc ttcgcaactg gtagtgggcg aatacatttt gcgagaagtt gaaaagacgt
    16381 tatgtgaatt gagatttctc cagttttagt tgtaaataaa tatccctcaa gacctatatg
    16441 gtctcctaaa tctaaagttt taaaaagatg ataattttcc tcgccaagat ctttttcggc
    16501 tgcatacatt tgtatgattc catctcgatc ttttagatga gcaaaactag ctttacccat
    16561 gatacgcttt gagtggattc ttcctgctaa tttatgtaga atatgaattt tagaagtatc
    16621 tgcatttgga tttttttctt tctccatttt ttgcagagtt tcgttttcat tcataataag
    16681 tgtataattc ttgattaaat ccacagaata actatcagga aaaaaacgga ttggataagg
    16741 gttgattccc ttttgtttta attcttcaat tttctctatt ctttgtttga ttaattcgtt
    16801 ttgctctaat tcgtgttcgc tcatggtaat attcaaaatt taggaaagac atctagagtc
    16861 aaggtgaaaa ccgcttaatc ttgcttagac ttttagatat ttgcctttat tttagaagta
    16921 agctatcttc agttaaatag ggagcaaaaa tcaaatttgc cgtaaatttc ttgacttgtt
    16981 tagaaataga tcaaaaatat ttactcttga atgaggtaaa cctatgaaaa aatttatttt
    17041 atattgttta gtaataacag tacttccagt ttttagtatt gagaaaattg aacctgatcc
    17101 aaaggcactc aagctagtct ctgattttat gaatgctctt acaattgcag atgaagcgga
    17161 gcgtctccaa gcgattcttc cacttgtgca tgacagttta aagacaaagg ataaaaagga
    17221 tttattttct agtgtaaaac aattttccta taaaagagca gtagatatga ttaaactata
    17281 caaagcaccg gcagaagttt cagaagtcca taaaggtgct gtttcaacta tcggttataa
    17341 agagttagct gaaaaaggta gaagagataa atatttcgtt aaaaagaaag agggagtcaa
    17401 tggtatgcca gctcctattc atgtatttat tccagagaat ggaggagagc caaagattta
    17461 cgactttgga agtttataaa atctttttga caatctctta gtctttctaa ttctatccta
    17521 tcatgaatat agaactaaaa agaattcaag agccattttt atttgaagca aaaaacgcca
    17581 caggcaattc tattcatata gatgcatctc cccagattgg aggaacggac aaaggcgtaa
    17641 gacccatgga acttttactc atgggtcttg cgggatgcgt tagcatagac gtaatcaata
    17701 tattgagaaa acaaagaata gctgtcttag attataatgt aaacgtagaa ggggaacggg
    17761 agacaggaaa agatgcaaac ttatttactg aaattaccgt acactttttt gtaaaagcag
    17821 atgtggaaga agataaactc attcgtgcaa tcgaattatc cctcgataaa tattgctcag
    17881 ttgcaaaaac tctagaaaaa accgcaaaaa taaaatacac ttttaggatt gagaggtaaa
    17941 tatgaattat ccgcaatcag agcattttga gacaaatgca atcagaattc aatccgataa
    18001 gactcagtat aga
//
LOCUS       DAOQOS010000113        17732 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_1602_length_17732_cov_7.000962, whole genome shotgun sequence.
ACCESSION   DAOQOS010000113 DAOQOS010000000
VERSION     DAOQOS010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 17732)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17732)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17732
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1602_length_17732_cov_7.000962"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            119..343
                     /locus_tag="PLF66_19805"
     CDS             119..343
                     /locus_tag="PLF66_19805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66346.1"
                     /translation="MNFGEGIQKISLVSEPLAYVRPIYTYAPIEQVNKIYSSDNYFNS
                     SAFKEFKNGNKQPNAEKKTMDRGAILDLLA"
     gene            complement(332..892)
                     /locus_tag="PLF66_19810"
     CDS             complement(332..892)
                     /locus_tag="PLF66_19810"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008589922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HMZ66347.1"
                     /translation="MLILKSASPRRREILEKLKLQFQVEPSNINEDKLERETPLDYLK
                     RVTIDKLEIEKSHSENIYISSDTMVVYENSILGKPVNEAEALQILSTLSGKTHSVYSG
                     IALWEKGDIFFGYEETRVEFKEWKEEEILNYIRNCKPFDKAGAYGIQDSISPVKRYIG
                     SYTNVMGFPIRTFYTRYPQWEKYLSK"
     gene            940..1929
                     /locus_tag="PLF66_19815"
     CDS             940..1929
                     /locus_tag="PLF66_19815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66348.1"
                     /translation="MKNHTLHFNIHFFKLNLVLITVFLFLFQSNLFGQTVSVKDTKNA
                     PSSDPAKTTPEKKEEGKPLKMETGTVVLDPQVVSADYKRTNLKHLKKMKSSFLNYEAE
                     PKFNAIMKNYVDASIKFQEKNYIASRRIFEQNYAELNTEAEGFSKKYSETYSKLYADA
                     SFQLVDLKVNSDSEESFYPVLEKQLMIANEFTSSAQIFTAKGNHVDALYEHKNALQSL
                     FKIYYTINKHKNKNLKLAERVSKNLLLDDDYIPKEYLKEYDDSLGLVFSVREKEREKE
                     REQIKKGLTNKYGELNLSKENESKQPAEPKKDTKDAKEVKDAKAPANPEPAKK"
     gene            1931..2182
                     /locus_tag="PLF66_19820"
     CDS             1931..2182
                     /locus_tag="PLF66_19820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66349.1"
                     /translation="MKKILFLLTIGLALLQTNCNSKYKTVDINDCDTIGNYYENGEHF
                     IRRDLFNDDGVIDYNKMLKSYKGRDTECNPLNQQFNTEK"
     gene            2179..2787
                     /locus_tag="PLF66_19825"
     CDS             2179..2787
                     /locus_tag="PLF66_19825"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004754026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HMZ66350.1"
                     /translation="MILHLHPKNPEIRTLKSISENLKEGEVYIFPTDTVYAIIADSKS
                     KKGIDKLHELKHSDKHKPLSLLCLDISTASEYIEQLPNDAFKLMKRITPGPFTFIFKA
                     NRNLPRWAVVNPKAKTIGVRIPENIFLQELLKVHDGTLTSTSVFTGDEYLTDIDDLEE
                     LFGNQVSGIVDGGIAKVEMSTVLDFTKDEMEIVREGKGFDRI"
     gene            2792..3361
                     /gene="thpR"
                     /locus_tag="PLF66_19830"
     CDS             2792..3361
                     /gene="thpR"
                     /locus_tag="PLF66_19830"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HMZ66351.1"
                     /translation="MSLFLAICPPESIREKIFTTIQNKDIPEELWERKEELHITLLFL
                     SKGNPDSEYLQSIKERLVRFPFSSFELQIGGLNLWSSKEYQILHLKIANSDELISIQS
                     RIITLFPEIKKSIHEFSPHISLIRSSGISKQKAEELKNKFSNFSTESFLVDKIHLYKG
                     EKDPKTKRNRYRILESFPSDESIRRIPQW"
     gene            complement(3501..4184)
                     /locus_tag="PLF66_19835"
     CDS             complement(3501..4184)
                     /locus_tag="PLF66_19835"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HMZ66352.1"
                     /translation="MKKKVSSKKTNKVPKSKKAEVQAGNLRELILDESIKIISESGVL
                     SLSLREVARKLGVSHGAPYRHFPTKDDLLAGIAAKGFKLFTEYLKEGLKEPDNLEISL
                     NGFLRMKSNYLKFAHENPDLFKFIFSASVPNDPKYEDLHHWSKASFEVLMRQLTSLRE
                     FGVLKKGNLFECAMFCSITMQGLALNQINHISDYLSLKYEYSGNLLDYLDTYILSGMN
                     AILEKKSNV"
     gene            4370..5095
                     /locus_tag="PLF66_19840"
     CDS             4370..5095
                     /locus_tag="PLF66_19840"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HMZ66353.1"
                     /translation="MKVVYILIFCIFACSSYETKTPRSVQVLSFNTGMANVYLLKGKI
                     NVMIDAGEPSDEKKIELFLKENGISPKDVSLMILTHGHEDHSGTASYFQKTYGIKLIG
                     GRGDKTLFATGKNDELKPTGVFGSIVKLFVKNDYPAIVPDIWIDSNYSLAEYGIEGEI
                     IPLGGHTDGAVAVILEREFAFVGDLLRGSPFSAHSPTEHFFHQDRQKVKNNIKILLDK
                     GIQVFYPGHWGPLKQEDVVKEFF"
     gene            5462..9028
                     /locus_tag="PLF66_19845"
                     /pseudo
     CDS             5462..9028
                     /locus_tag="PLF66_19845"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
     gene            9101..9739
                     /locus_tag="PLF66_19850"
     CDS             9101..9739
                     /locus_tag="PLF66_19850"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HMZ66354.1"
                     /translation="METACYRQLGRLVGKEEIQPDATQTAWTFPQKDSGAREDLESFS
                     LKIQANKKEADGIMAENYPEFYAKGNLLYNDQGNIQPFSYSQVAIGVKWKILDGGTIL
                     TRHKQKLEEILSLEEQYKDAIKGVSVGITDAKAHLASAQKNYSQLKEELAETDKVIQQ
                     EMYRFLAGRTSSNSYLQVKELWLSKQELLKMKKIEMLRWEITLSFIEAKLGD"
     gene            complement(9856..10203)
                     /locus_tag="PLF66_19855"
     CDS             complement(9856..10203)
                     /locus_tag="PLF66_19855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004495164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HMZ66355.1"
                     /translation="MKVYEYKGCGTCRKALKFLDSKKISYTKLPIRETPPSLSELKKA
                     LKIIGDSKKLFNTSGQDYKSLNLKDKLSSMTEDEKLNLLHSNGNLVKRPFVVLEDSIL
                     IGFKEEEWKKQFK"
     gene            complement(10252..10968)
                     /locus_tag="PLF66_19860"
     CDS             complement(10252..10968)
                     /locus_tag="PLF66_19860"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HMZ66356.1"
                     /translation="MNRQFIRTVLVIGIVSTSLAFCSGNDKKNSPDPKDAKTAEDTKT
                     TDRAAVEEPKDVKPVDAKDDKDSVAKRALTGAADELFAQLNESLKSARYPDGESIEGF
                     AYKKWEIPNKKDFVKWVKGAGAVLKKGLDELPAIYTLEIAGHTDTVGPENPEGAKKGN
                     MFYSEQRAKEVKQALVKLGFPEKRIATKAMGSSNPIPGIPGESAKNRRVTFQFLAGEP
                     EAKDSSATDAPKTDAKDESK"
     gene            11067..12005
                     /locus_tag="PLF66_19865"
     CDS             11067..12005
                     /locus_tag="PLF66_19865"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HMZ66357.1"
                     /translation="MIITVNVQEGQNDQRLDHFLAQSTGDEISRTSIQKWIKSGNVVN
                     LSNPKESKLKANLKVQIGDIFQITIPPKPKLNLTPVEMKLEILHEEEDFVIIDKPAGI
                     ASHGGPGDDGPTLVNGLLFYFKNLSKLGGDIRPGIVHRLDKPTSGIMIIAKTDKAHIQ
                     FVKMFQKREIEKQYYAWLVQTPKLPTGRIELPLGRHPTERLKMCVRNDGRKAITNYQI
                     EKSISSRKNRNYSLAKIQIETGRTHQIRVHFQSMGCPVVGDMLYSRTGNEFSQYGLLL
                     FSQKIRFKHPFTKKIIDISLPFPERFTRFEKEAEFR"
     gene            complement(12206..14281)
                     /locus_tag="PLF66_19870"
     CDS             complement(12206..14281)
                     /locus_tag="PLF66_19870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66358.1"
                     /translation="MFYFFKKKEPKLPTEYILENSKIKMEFDREGKILALTDKAVNHC
                     YSMIASNSIESFSIQELSSIQKDTSIEFEKKLYSTNGRTSVLKEKYTLGDKSISLDIL
                     IESAYPFWTQDIITKIQYEVSKQTRFWTAWSDPFQKNSGWHDPRVFLPFSNKLWEYSN
                     LSHYTPEKGNFISIPIATIAEINTDNALSIYLSPKDKIFQLYLDTDKNGELCFIRRGN
                     RLGGGKQVSFSLDFGFHEADWRGGLRYYTEKYPEYFDPKNTKANEMAGCGAYSGYEGE
                     IDSEKLSKMGFRINWKLSDDFPWMGMFLPPVKSKEEKWKRSCDEPNPSGKSEYTSCEQ
                     MNEYAIQMKKFGFYVLNYFNVTEFGKNKNASEWNKEFPEEEIWKNPIQFLKRYFPNAV
                     LDPKLRTFYGAYVMDSGEEGYRDFLIEQAKRHIEMLPDTDGICIDRLDWLRYFNPNAD
                     DGVSMHQNKITRSLVESWNTLIERLASILHEKGKVIFTNPMTMRLDLYKEVDGFYSEH
                     GESGPGLNSLAWIGMRKTSLTWTCMWWYIPTYKLKPDPDSFFQRHLLMGVFPTAPYPF
                     NNHALRPNAKTDQYYLDYGPMLLALRGKKWVLAPHCVVCEDPKVKVNLFETLEGYALP
                     VTFAFHRKEVEVWIQNLPDLKTFEATVCYPAKEVKRIETIVQENKILIRVPIVRGCAL
                     IKLKKKRLQ"
     gene            14371..14823
                     /locus_tag="PLF66_19875"
     CDS             14371..14823
                     /locus_tag="PLF66_19875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66359.1"
                     /translation="MKQEKILNLSKILLTIGALEFFGPIARDTNSSHLLNDAWVGHAR
                     FHLMWNICLWASMGIYSIYILWGKKEVRIKDLYTCFSLQVMNAIGFWGSVLLSGIYQG
                     DIFDASIHVGIMNVNENILVFSLLSSLLLINYIILKKGIEPSFKEVNV"
     gene            14820..16640
                     /locus_tag="PLF66_19880"
     CDS             14820..16640
                     /locus_tag="PLF66_19880"
                     /inference="COORDINATES: protein motif:HMM:NF013646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HMZ66360.1"
                     /translation="MSFINQMKTEVLIIGGGPTGMVASIALSKQGISNILVEKRDKPM
                     LHPKAHELSARSIEILDSLGIPFSEIQKEASDHETASRILFCNTINEEFGRIDLRENG
                     TDEKYADNICYPHPYFNISQVELEKIIRNYMLRCKEGSALYGYQWESFKEGREWTSSD
                     VFNTVTGERIKIKSKFVICADGATSKGREALQIKMIGPEKIQDFANAYFINDLTPYLK
                     TKAKLYWILNPEAPGVLIAHHPEKRWVYHVPIETPYEKLEDFTVEYFSKRIKKALNLK
                     ENIPIHITSISSWRMTAQVAEKFRAGSIFLAGDAAHRFPPTGGLGMNSGIADVHNLAW
                     KLAAVIKGFAGNSLLDTYEAERKPVIERNCFESKKNSNDILDVPASLGLDIKKGRELA
                     RILHSNFIHSLPKSWGDKLKSILTLPTRLRLKIFLRNSENFPKLKTIIKKQTGHFDRI
                     GLDLGFSYKSEAIISDGISKEEELEVSNYFPTTKPGHRFPHFWLNSQLSSQALLSYKN
                     FTLILGSKENKWGSALKKINLDIPITVSFLSEYNENLGELYKKAEIESTGAILVRPDG
                     QVAYRKQFLSKGEDPYKILESIFQTILSSRQEKINKDVAA"
     gene            16637..17617
                     /locus_tag="PLF66_19885"
     CDS             16637..17617
                     /locus_tag="PLF66_19885"
                     /inference="COORDINATES: protein motif:HMM:NF013706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HMZ66361.1"
                     /translation="MKVFLILAIILLLPSLGFFYYLFRPLRIVPKPAAFQCIPIQDGK
                     LEAFTPNPNHIFGMGLVYSKHIEETASNFSIVEPPPIFIKDKQSLASHNSEVKTPNSL
                     DLFQALLKYEPSLESEEGLVNNKDLPPLLDYEVELGFVLLADIKKEDLYKKEFIPPIG
                     FFLANDISARTIAVFGEGRQDKAEYWGISKSFPNFLPVSSQVWIPSHGLPNGIPCIRI
                     QTFVNGIERQNEVTVNMIYTPKEMLQFISEKYKNIPLKKNDMVIMGTPGGVAMSTPRW
                     MVRISDLIGLNRFQKLKIVLRKDRTNFLKSGDKVLIRGEGFGEVENTIVN"
BASE COUNT         5981 a         3103 c         3070 g         5578 t
ORIGIN      
        1 tgatagtcag aggtaaactc atgaatattt ttatattcag tgatttcctt tttttttgct
       61 tgtttttcag ccataatttt agtacattaa ccttgttgta caaaaagagg taaagaatat
      121 gaatttcgga gaaggaatac aaaaaatctc attagtatct gagccattag cttatgttcg
      181 tccgatttat acctacgcac ctattgaaca agtaaataaa atctattcaa gcgacaacta
      241 ttttaactct tctgcattta aagaatttaa aaatggaaac aaacaaccaa atgcagaaaa
      301 gaaaacgatg gatcgtggag caattttaga tttacttgct taaatatttc tcccattgcg
      361 gataacgtgt gtaaaaggtt ctaatcggaa aacccatcac atttgtataa gaccctatgt
      421 atcttttcac tggacttatt gaatcttgaa ttccataggc gcctgcttta tcaaatggtt
      481 tacaatttct aatatagttt aaaatctcct cttccttcca ttccttaaat tcaactcttg
      541 tctcttcgta tccaaaaaag atatctcctt tttcccataa ggcgattcct gaatatacgg
      601 aatgagtctt ccctgaaaga gtcgaaagaa tctgcaatgc ttccgcttca ttgactggct
      661 tacccagaat cgaattttca tagactacca ttgtatcaga acttatatag atattctcag
      721 aatggctctt ttctatttcc aatttatcaa tcgtcacccg tttcaaatag tcgagtggag
      781 tctctctttc taatttgtct tcattgatat tcgaaggttc tacttgaaat tgcaacttta
      841 gtttttctag gatttcacgc ctacgaggag aggctgactt taaaattaac attgatttac
      901 cctttcggaa acattaaaaa tgtcgataat aagtacacta tgaaaaatca tacactccat
      961 ttcaatattc atttttttaa actcaatcta gttttgatca cagtatttct gttcctattt
     1021 caatcgaatc tatttggaca gactgtcagc gtaaaagata caaaaaatgc tccaagcagt
     1081 gatccagcca aaacaacacc agagaaaaaa gaagaaggca agccattaaa aatggaaact
     1141 gggacggtag ttttagatcc tcaagttgtc tcagctgact acaaacgaac taatttaaaa
     1201 catttaaaaa aaatgaagag ttcttttcta aactatgaag cagaaccaaa attcaatgca
     1261 atcatgaaaa attatgtgga tgcgagtatc aaatttcaag agaagaatta cattgcatct
     1321 agaagaatct ttgaacaaaa ctatgcagag ctgaatacag aagcagaagg cttttctaaa
     1381 aaatattctg aaacatactc aaaactctat gcagatgcat cttttcaact agttgactta
     1441 aaagtaaatt ctgattcaga agagtctttt tatccagtct tagagaaaca attgatgatt
     1501 gcaaacgagt ttacttcctc tgcgcaaatt tttactgcaa agggaaatca tgttgatgcg
     1561 ttatacgaac ataaaaatgc actgcaaagt ttatttaaaa tctactatac aatcaataaa
     1621 cacaaaaata aaaatctaaa gttagccgaa agagtaagca aaaatctttt actagacgat
     1681 gactatattc ctaaagaata tttaaaagag tatgatgact ccctcggatt ggttttctct
     1741 gttagagaaa aagaaagaga gaaagagaga gagcaaatca aaaaaggtct cacaaataaa
     1801 tacggtgagt taaatttatc caaagagaat gaaagcaaac agcctgctga accaaagaaa
     1861 gatacaaaag atgcgaaaga agtaaaagat gcaaaggctc cagcaaaccc agaacctgct
     1921 aaaaaataaa atgaagaaaa ttctatttct actcacaatt ggtttggctc ttctccaaac
     1981 caattgtaat tccaaatata aaaccgttga tatcaatgat tgtgatacta ttggaaatta
     2041 ttacgaaaat ggagaacact ttattcgaag agacttattt aatgatgatg gtgtgattga
     2101 ttacaataaa atgctgaaaa gttataaagg acgagatact gaatgcaatc ctttaaatca
     2161 gcaatttaat acagaaaaat gattttacat ttgcatccaa agaatccaga aataagaact
     2221 ctaaaaagta tttctgaaaa tctaaaagaa ggtgaggttt atatttttcc aacagataca
     2281 gtctatgcaa tcattgcaga ttctaagtcc aaaaaaggaa tcgataaact tcacgaactc
     2341 aaacactccg ataaacataa accactttcg cttctctgtc tagatatatc cactgcatcc
     2401 gaatacattg agcaacttcc caatgatgct tttaaattaa tgaaaagaat cactccaggt
     2461 ccttttactt ttatctttaa ggcaaatcgt aatctacctc gctgggctgt agtgaatccc
     2521 aaagcaaaaa ccattggagt tcggattccc gaaaatattt ttttacaaga acttctaaaa
     2581 gttcatgatg gaacgttaac ctccacttct gtcttcactg gagatgaata tctaaccgat
     2641 atagatgatc tagaagagtt atttggaaac caagtaagtg gtatcgttga tggcggtatt
     2701 gccaaagtag aaatgtctac cgtcttagat tttaccaaag acgaaatgga aatagttcga
     2761 gaaggaaagg gtttcgatcg catttagcct tatgagcctc ttcctagcaa tatgccctcc
     2821 agaatccata cgagaaaaaa tttttacaac catacaaaac aaagatattc cggaagaatt
     2881 gtgggaaaga aaagaagaat tgcatataac gcttcttttt ctctcaaaag ggaatcctga
     2941 ttcagagtat ttgcaatcaa taaaagaaag gttggtgcgc tttccatttt catcttttga
     3001 attacaaatt ggaggattaa atctttggtc atcaaaagag tatcaaatcc tacatttaaa
     3061 aatagcaaat tcggacgaat tgatctcgat tcaatcaaga attattaccc tcttccccga
     3121 aattaaaaaa tccattcatg aattctcacc acatatctcc cttattcgtt cttctggaat
     3181 ttcaaaacaa aaagcggaag aattaaaaaa taaattttcc aatttttcaa cagaatcttt
     3241 tctagtagat aaaattcatt tgtataaagg agagaaggat cctaagacaa agagaaatcg
     3301 ttatcgaata ttagaatctt ttccaagtga cgaatcaatt agacgaatcc ctcagtggta
     3361 gagtaaataa aatcaattag ctaaccaacg catttaaaat aatctcttct gaaaattttg
     3421 gagaatttga aacgatataa ttcttgatct agaattataa aagatttata ttcttagatt
     3481 ccaacttatt cgtttaactt tcatacatta cttttctttt ctaatatcgc attcatccct
     3541 gataaaatgt atgtatctaa ataatccaaa agatttcctg aatactcata ttttaaagat
     3601 aaataatcag aaatatgatt gatctgattc aaggctaagc cctgcatcgt aatcgaacag
     3661 aacatagcgc attcaaatag atttcctttt tttaaaacac caaattctct cagactagtt
     3721 aattgtctca taagcacttc aaaggatgct tttgaccaat gatgtaaatc ttcatattta
     3781 ggatcatttg gaacagaagc gctgaatata aatttgaata gatccggatt ctcgtgtgcg
     3841 aattttagat agttggactt cattcgcaaa aagccattga gagaaatctc taaattgtct
     3901 ggctccttta aaccctcttt cagatattcc gtaaaaagtt taaatccttt ggctgcgatt
     3961 ccagctaata aatcatcctt ggtaggaaaa tgcctatacg gtgctccatg agaaacacct
     4021 agttttcgcg ccacctcccg taagcttaaa gagagaactc ctgactcaga aatgatttta
     4081 atggattcat ctagtatgag ctcccttaaa tttcctgctt gcacttctgc tttcttggat
     4141 tttgggactt tatttgtttt tttggatgat actttctttt tcattatgca gtgatttaaa
     4201 ggaaatgtga gttttattta tttgtctatt attttttatt tgacttattt attcagtaga
     4261 cactgtatac tattctgtag acaatgtaat tttcaaatga tttaaaaata aatttattat
     4321 tatataattc tctaaatttg atttgtccta ctccatttta ggagaccata tgaaagttgt
     4381 ttatattttg attttttgta tttttgcttg ttcctcttat gaaactaaga cccctcgaag
     4441 tgttcaagtc ctttctttca atacaggtat ggcaaatgtt tatttattaa aaggtaaaat
     4501 caatgtaatg attgatgccg gagagccttc agacgaaaag aaaatcgagt tatttctaaa
     4561 agaaaatggg atttctccaa aggacgttag tttaatgata ttgactcacg gtcacgaaga
     4621 tcattcagga acagcatcct actttcaaaa aacctacggt ataaagttaa taggaggaag
     4681 aggagataaa actttatttg ccacaggaaa aaatgatgaa ctaaaaccca ctggtgtttt
     4741 tggttccatc gtaaagctat ttgtaaaaaa tgattatcca gctatcgttc ctgatatatg
     4801 gatagattca aactactctc ttgctgaata tggaatagag ggagaaatca ttcctttggg
     4861 tggccataca gatggggctg ttgcagtgat tttagaaaga gaatttgcct ttgttggaga
     4921 cttactcaga ggttcccctt tttcggcaca ttctcccaca gaacattttt ttcaccaaga
     4981 cagacaaaag gtaaaaaata atattaaaat acttttagat aaagggattc aagttttcta
     5041 tccaggacat tggggaccgt taaaacagga agatgttgta aaagagtttt tctaatcatt
     5101 cccacaattg acttatctaa ttcaattcct atttaatgaa ttggtttgga taggtctgtt
     5161 atttactatt agataaagtt taatctatca aaaaccgcaa aacttttatt ccacaagttt
     5221 tcaatccgtt ttatacccta cccttataat ctaagctaat caaaagagaa gagttctatt
     5281 cactttttac aaataagaaa atttctcctg agacttttat attttacttc gacttccaat
     5341 ctagtttaaa cttttttatt tatctattag taattttgta gacaatgttt actattattg
     5401 gtaagtgtct actttttaga tgacactgtc tactggagga tttatgaact ggtcaacact
     5461 aatgattcaa aaatatcgct ttttttggct tttaggaatt tcttttgttc ttttgggttt
     5521 tctggcttgg ttccaaatac caaaggaaga agatccgaga ttaaagccgc gttttggctt
     5581 aattaaactc atctatccgg gggcgtctgt aataagaatg aaacgtttgg tagttttaga
     5641 agtagaaaaa gaactagcaa gtgttgattc catcgaaaca atctctacta ccatacgatc
     5701 tgacttcgca gccattgaag tcgttttaaa agatcgactg agagaaaatt cagaaatcac
     5761 aagagcctgg gatgaagtag aggaagcttt aaataaagct aaaaataaat ttccaagtgg
     5821 tgtatttcct cctgaattag atcgaaaact aatggatcaa gaagcaatcc tattaagtat
     5881 tgaaggagac gataggtata aacatgatat actcattgag ttagaagaca aacttttgta
     5941 aaaatctact gtctccaaag taaaaagagt tggagatgaa ggaaaagaaa tcagaattat
     6001 tctagatgaa gttaaagtaa aacaaattgg attaaattta aaagacgtta ttgcacagat
     6061 ccaagctgga aatgccgaaa tttcttccgg ctatattgaa gagaacggaa acaaattaaa
     6121 tatcggttct actaattctt tctattctgt tactgagttt agtaaattcc cgatcgcttc
     6181 tcctaacggt agaagcattc cgctctcctc atttgctaaa gttacagaag aagagatcca
     6241 accaaaaaaa gaagaaatgc actggaatgg aaaactggta tatggattag gagtaattcc
     6301 aaaacaagat attgacctta gaaattttgg aacagacata acgcacgaaa tagaaatttt
     6361 taatttacga ttggtcgaaa aaaatattaa cattagaatt aatctaatca attctcaacc
     6421 aaagtttgtt tccaatcgct taaatgagtt agctttttct ttattacaat caattctgat
     6481 cctagcctct tttcttatga ttcttatggg aatcagagtt gggcttgttg ttagtataat
     6541 gcttccagtg attagtctta taactttagc actatactat ctcattggag gaatcctaca
     6601 tcagatagca atcgctgcct ttgttatggc tttgggttta ctcattgata atgtaatcgt
     6661 aattgtagag agtgtccaag aaaaaaaaga aaagggattg gactctattt ctgcaggaag
     6721 tgaaacgatt aaagaattca cagcacctct tttctcttct actcttacaa ctatcgcaac
     6781 attcttgcct atgtggatgt caaagggatc tagctcagaa tttacagaag cgattccaaa
     6841 aattacaatg atttctttgg gagtgagtta ttttgctgct ctttttcttt ctccaagtct
     6901 atctcttttt ttactaggaa atagcaaaga gaacaaatta cctttcttag ataaattagg
     6961 gaatttcgtt gcaaatgttg tgatcgaaaa accatttcga atcttgatcg gctttggaat
     7021 attctttcta atctttatta gctgcattgg atttatgaaa ttcaagtttt tcccatcagc
     7081 ggatagaaat caagtcatag ctgaaatttc ctttccagaa ggaactgcca tttctaaaac
     7141 aaaagaggca gtagaaaaat ttgaacaatc cctactaggt catgaggaag tttctcaaat
     7201 cacttctttc acaggtagaa gtacaccttt tttctattac aatttaaatc aaaagccgag
     7261 tgctcctcat ttagctcaga ttctaatcga atccaatcag acaataattt ctgaaaaatt
     7321 aaaagctcgc atctatgaag taggaaatag tctaggagca agatttaatg taagaagttt
     7381 tgaacaaggt cctccagtag aagcatcttt aatcattcgg ctttatggag aagacagagt
     7441 gcaatctacc aaagaagcag aaagaattat aaagtatttg gaaaaaaatc aaaacataac
     7501 gcaagcttgg cacaacgcac taggagaaca aaaagaggca atctttaaat cagatgatcg
     7561 atttttaata gagagaggat tgacgagaca agatctttct cttgctcttt tgaaggtaac
     7621 gaatggaatc ccattaggtt tttacaaagc agaaagagag agtgtgccta tcgttctctc
     7681 atctggaaaa ttggaaaagt caaatatttc cgatctaaaa aatgctcttg ttggacaaac
     7741 tttggattta ccgattttag cttcctccgt aagtaaagtt gaattcaaag atgtcacacc
     7801 tgttttttat tctagaaatc gaaaacaata cattgccgtt ctagctacta taaaatatgg
     7861 aattgaccct ggaatggttg caagcgaagt cgagagcttt ttaaagaaag aaccattacc
     7921 aaacggaatc gattgggagt ttgatggcga agcaaaggaa agtgaaaaat ctaactcagc
     7981 tttagttgcg gccttacctt taggaattat cgtattagtt gcgactcttt tatgggaatt
     8041 taatagtatt agattgactt taatcatact cacatcagcg cctaccgcat ttatggggat
     8101 cgttcctggc ttagtattct ctggaaagcc tttcggtttc ttatctctac ttgcactctt
     8161 tgcactgata ggaatagcag tcaacaatgg gattatttta atagataaat ttagactttc
     8221 agaaaaggaa ggcgttattc cagaacttgc agttcgaact ggaatttcaa tgaggcttcg
     8281 tcctatacta ctcactactg gatctacaat tttgggcatg gttcctctta ctttttctag
     8341 ttctactttg tggccacctt ttgcttggtc tatgatttca gggctcacta tatcaactct
     8401 ctttacttta tttttagtgc cttggtttta tttaaaatat gcaaaagcta aagggaaatt
     8461 ttctatacct aaacttttta ctgcattgat tttcttaatt ttgtgttttg gagaaattca
     8521 ctcccaagaa atgaaaccta caatcaactt agagaaaatc aacttattcg atgcaatcac
     8581 gttagccgca aactcatctg ctgcgaaaag tgcctttcat gatgctgctg cagctaaata
     8641 tggatctaaa accgcaacct acgcagctta cgttccaaaa ttcggattgg ggggagaacg
     8701 tatatttagg gataaatcat tacaattaga atctccattt ggaaatcttc catttgcaag
     8761 tcgtgctttc tatcaagcag gtatggagct ttatcaaaca ctttggagca cagatcttat
     8821 ggaattcaaa gtgccggcag caaacctgat ggcagaagca gccgccaaga aatctatctg
     8881 ggaaagacga agtgcgcaac acaatgcagc catggcctat cttgattgcg taacactcat
     8941 catccaaaaa gaatctttaa aagagagaaa gaaagtattg ggtcaattat cgaaagagct
     9001 gtctagtctg tcgtataacg gtaagataaa agaagcagat tctcttaaaa tcaaattctc
     9061 gattggagat atagatagag gaattactga aattgaattt atggagacag cttgttatcg
     9121 tcaactaggc agattagtag ggaaagaaga gattcagcca gatgcaacac agactgcatg
     9181 gacttttccg caaaaagact caggtgctag agaagatttg gaatcctttt ctttgaagat
     9241 acaagccaat aaaaaggaag cagatggaat catggcagaa aattatcccg aattctatgc
     9301 aaagggaaat ctactctata acgatcaagg aaacattcag ccattttcct actctcaggt
     9361 tgccattgga gtaaaatgga aaatccttga tggtggcaca attctaacaa ggcataaaca
     9421 aaaattagaa gaaatactct ctttagaaga gcaatataaa gacgcaataa aaggagtatc
     9481 tgttggtata acggatgcaa aagcacatct tgcctcagca caaaaaaatt attctcagtt
     9541 aaaagaagaa ttggcggaga cagataaagt aattcaacaa gagatgtata gatttcttgc
     9601 aggtagaaca agttccaatt cctatttaca agtcaaagaa ctctggctat ctaagcaaga
     9661 actattaaaa atgaaaaaaa tagaaatgtt acgttgggaa ataacgcttt ctttcatcga
     9721 agcaaaatta ggagactaaa aacggtaaat tatagagttg atagaagtat ccttttttat
     9781 tcttcaggaa tctgcaaaga taccttcaaa atatagagct agcaatttga attactcgac
     9841 tttcgtaaaa attgattatt taaattgctt tttccattcc tcttctttga aaccaattag
     9901 tatggaatct tccaatacaa caaaaggacg ttttactaag ttgccattgg aatgaagaag
     9961 atttaatttt tcatcctctg tcattgagga caatttgtct tttagattga gagatttata
    10021 atcctgaccg gatgtattaa aaagtttttt actatccccg attatcttta aggctttttt
    10081 taattcagag agagagggtg gagtttctcg aatgggtagt ttggtatagg aaattttctt
    10141 tgaatctaga aactttaatg ctttgcgaca ggtgccacag cctttgtatt catagacttt
    10201 catagtagga agggaatgct gccaaagatt ggcagcactt aggttgtaat cttatttaga
    10261 ttcgtctttt gcgtcagttt ttggagcgtc tgttgcggaa gaatctttag cttctggctc
    10321 acctgctaag aattgaaatg taacacgtct atttttagca ctttctccag gaattccagg
    10381 aattggattg gaagaaccca ttgcttttgt agcaattctt ttttctggga aacctaattt
    10441 aacaagagct tgtttcactt cttttgctct ttgctcagag tagaacatgt ttcctttttt
    10501 agcgccttct ggattttcag gacctactgt atcagtgtgt ccagcgattt cgagagtgta
    10561 aatagcaggg agttcatcca agcctttctt tagaaccgct ccagcacctt ttacccattt
    10621 tacaaaatct tttttatttg gaatttccca ttttttgtaa gcaaatcctt caatagattc
    10681 tccgtctgga taacgagctg attttaatga ctcatttaat tgagcaaata actcgtccgc
    10741 agcaccggtg agagcacgtt ttgcgacaga gtctttgtca tcttttgcat ccactggttt
    10801 tacatctttt ggctcttcta cagctgctct atcagtggtc tttgtatcct ctgcagtttt
    10861 tgcatcttta ggatcaggag agtttttctt atcatttccg ctgcaaaatg ccaatgaagt
    10921 agaaacaatt ccgatcacta ggacagttct aataaattgt ctattcataa ataaatgctt
    10981 cctttctaat aatgattttt gaatattacc acaatagaaa tgtctatttt attttaaaac
    11041 catttttaat tgataaatta attttcatga taataaccgt aaatgttcaa gaagggcaaa
    11101 atgaccaacg tttagatcat tttttagccc aatccaccgg agatgagatt tccagaactt
    11161 ctatccagaa atggattaaa tctggcaatg tagtcaatct ttcgaatcca aaagaatcca
    11221 aattgaaagc caatctcaaa gtgcagatag gcgacatatt tcagattacc attccaccga
    11281 aaccaaaact aaacctaact cctgtggaga tgaagctcga aatcctccat gaagaggaag
    11341 attttgtgat tatagacaaa ccggctggaa ttgcctcgca tgggggacct ggagatgatg
    11401 gacctactct tgtgaatggt cttctctttt atttcaaaaa cctttccaaa ctaggagggg
    11461 acattcgtcc tgggattgtg caccgtctag acaaaccaac atctggaatt atgatcattg
    11521 ccaagacaga caaagcacat attcaatttg tgaaaatgtt tcaaaaaaga gaaattgaaa
    11581 aacaatatta cgcttggctt gtccagactc caaagctacc aaccggacga attgaacttc
    11641 cacttggcag acatccaacg gaaagactca aaatgtgtgt tcgaaatgat ggaagaaaag
    11701 cgatcacaaa ttatcaaatt gagaagtcta tctcttctcg taaaaataga aactactctc
    11761 tcgcaaaaat ccaaattgaa acaggaagaa cgcatcagat tcgagtgcat ttccaaagta
    11821 tgggttgtcc tgtggtggga gatatgctct attcaagaac gggaaatgaa ttctctcagt
    11881 atggactcct tcttttttct cagaagatac gattcaagca tccttttact aagaagataa
    11941 tcgatattag cctccctttc ccagagcgct ttactcgctt tgaaaaagaa gcagaattta
    12001 gataaaaaaa catctataaa aaaagattta aaagattacc acctattgcc gcgaacgagc
    12061 ggcaatggat tttgggcgga aagacgatga gcacggataa aagataagat aattcaaaat
    12121 tatcaatcgt ttctgtatcg ttcagttatc ggtgtcctcg gtggtaattc ttattgatcg
    12181 acgcctttgt atgtggtaaa aaattctact gcaatctttt cttctttaat ttgataagag
    12241 cacagccgcg aacaatagga actctaatta gaatcttatt ttcttgaaca atagtttcta
    12301 tcctttttac ttcctttgca ggataacaaa ccgttgcctc aaaagttttt aagtctggaa
    12361 ggttttgaat ccatacctct acttcttttc tgtggaaagc aaaagtcact ggaagtgcat
    12421 acccttctaa tgtttcaaat aaatttactt tcacttttgg atcttcacac acaacacaat
    12481 ggggagccaa gacccatttt tttccacgca atgctaagag cataggacca tagtctagat
    12541 aatattgatc tgtttttgcg tttggtctta aagcatgatt attaaaagga taaggagccg
    12601 ttggaaaaac tcccataagc aaatgccttt gaaagaaact atctggatcc ggttttaatt
    12661 tgtaagtagg tatataccac cacatacaag tccaagtgag agaggtcttt cgcataccga
    12721 tccaagccag agaatttaag ccgggtcctg attccccatg ctcagaataa aatccatcta
    12781 cctctttata taaatccaaa cgcatggtca ttggatttgt aaaaataact ttcccttttt
    12841 catgtagtat cgaagcgagt ctttcgatca aagtattcca agattctacc aaagaacgag
    12901 tgattttatt ttgatgcata ctcactccat catctgcatt tggattaaaa tagcgaagcc
    12961 aatcgagtcg gtcgatacaa attccatctg tatcagggag catttctata tgtcttttgg
    13021 cttgctcgat tagaaaatct cggtagccct cctctccaga gtccatcaca tatgccccat
    13081 aaaaagttct aagctttgga tcgagcactg cattcggaaa ataacgtttt aaaaactgaa
    13141 tcggattttt ccaaatttcc tcttcaggaa attctttatt ccattcacta gcattcttat
    13201 ttttcccaaa ctcagtcaca ttaaaatagt ttagcacata aaacccaaat tttttcattt
    13261 gaattgcata ctcattcatt tgttcgcatg aggtatattc tgatttgccg ctaggatttg
    13321 gttcatcgca ggatcgcttc cacttttctt ctttcgattt tacaggtgga agaaacattc
    13381 ccatccaagg aaaatcgtct gacaatttcc aatttatgcg aaatcccatt ttgcttaatt
    13441 tctctgaatc tatttctccc tcataaccgg aataggctcc acatccagcc atctcatttg
    13501 cttttgtatt ctttgggtcg aaatactctg gatatttttc tgtataatac ctaagtcctc
    13561 ctctccaatc tgcttcatga aaaccaaaat ctaaagaaaa agaaacttgt tttcctccac
    13621 cgagccgatt ccctcttcga ataaaacata gttctccatt cttatctgta tctaaataca
    13681 attgaaatat tttatccttt ggggaaagat aaatagaaag agcattgtct gtgtttattt
    13741 ctgcgatagt cgcaattgga attgaaataa aatttccctt ctccggtgtg taatgagata
    13801 agttagaata ttcccatagt ttattagaaa atggaagaaa aactcttgga tcatgccagc
    13861 ctgagttctt ttgaaatgga tcactccaag cagtccaaaa cctagtttgc tttgagactt
    13921 cgtattggat cttagtgata atatcctgag tccagaaagg atatgcactt tctattagaa
    13981 tgtccaaaga tatggactta tctcccaaag tatatttttc ctttagaaca gatgttctgc
    14041 catttgtgct gtaaagtttt ttttcgaatt caatgcttgt atctttttga atagaactca
    14101 gctcctgaat cgaaaaagat tcaattgagt tggaggcaat catggaataa caatgattta
    14161 ccgctttatc tgtgagagct agaatttttc cttctctgtc aaattccatt ttgattttgg
    14221 aattctctag aatgtattct gtaggtagtt taggttcctt ctttttaaaa aaataaaaca
    14281 atttgagtcc ttccttttaa ggcgggacga ccgcactcca agtttatgat aaactttacc
    14341 tatcatccag tgaatggata ggaggaaaaa atgaaacagg aaaaaatttt gaatctatct
    14401 aagattctat taaccattgg ggcattggag ttttttggtc ctattgcaag agatacaaac
    14461 tcttctcatc ttttaaacga tgcatgggtt ggacatgccc gatttcatct tatgtggaat
    14521 atatgccttt gggcatctat ggggatatac agcatttata ttttatgggg aaaaaaagaa
    14581 gttagaataa aagatctata tacttgtttc tcactgcaag tgatgaatgc cattggcttt
    14641 tggggatcag tgctcttgag tggaatctac caaggggaca tttttgatgc aagtattcat
    14701 gttggaatta tgaatgtaaa tgaaaacatt ctagtattta gccttctctc ttctcttctt
    14761 ctcatcaatt atataatttt aaagaaagga attgagccaa gtttcaaaga ggtgaatgta
    14821 tgagctttat caatcaaatg aaaacagaag ttctaatcat tggaggcgga ccaactggaa
    14881 tggtcgcgtc tatcgcactc tctaaacaag gaatttcaaa tattctagtt gaaaaaagag
    14941 acaaaccgat gcttcatccc aaagctcatg aactgagtgc tagatcgatt gaaattttag
    15001 atagtttggg aattcctttt tcagaaatac aaaaggaagc atccgaccac gagactgcct
    15061 cacgaatttt gttttgtaat acaatcaatg aagaatttgg taggatcgat ttaagggaaa
    15121 atggaacaga cgagaaatat gcagataata tctgttatcc gcatccatat tttaatatat
    15181 cccaagtaga attagaaaaa atcatacgca attatatgct aaggtgcaaa gaaggatcag
    15241 ctctctatgg ctatcaatgg gagtcattca aagaggggag agagtggaca agttcagatg
    15301 tattcaatac agtcactggc gagagaatta agatcaaaag taaatttgta atttgtgctg
    15361 atggagcaac tagtaaggga agagaagcat tgcaaatcaa aatgattggt cctgaaaaaa
    15421 tccaagattt tgccaatgct tattttataa atgatttaac tccttatctc aaaactaagg
    15481 caaaactcta ttggatccta aatccagaag ctcctggtgt cctgatagcg catcatccag
    15541 aaaaaagatg ggtctatcat gtgccaattg aaacacctta tgaaaaattg gaagacttta
    15601 cagtagaata tttttctaaa agaataaaaa aggcactcaa tttaaaagag aatattccta
    15661 ttcacataac atcgattagc tcatggagaa tgacagcaca agtcgctgaa aaatttagag
    15721 caggatctat cttccttgca ggggatgccg cgcatcgctt tcctccaaca ggtggattag
    15781 gaatgaattc tggaattgca gatgtgcaca atctagcgtg gaaactcgca gcagtgatta
    15841 aaggctttgc aggaaattct ctgttagaca catatgaggc ggaaagaaag ccagtaatag
    15901 aaagaaattg ctttgaaagc aaaaagaact cgaatgatat tttagatgtc ccagcgtcct
    15961 taggcttgga tataaaaaaa ggaagagaat tagctcgaat acttcattcc aatttcatac
    16021 attcattacc caaaagttgg ggcgataaat taaagtctat tttaaccttg cctacaagac
    16081 ttaggctgaa aatttttctc cgaaactcag agaattttcc caagttaaaa actattataa
    16141 aaaaacagac aggtcatttt gatcgaattg gtttagatct aggctttagt tataaaagtg
    16201 aggcaatcat ttcggatgga attagtaaag aagaggaatt agaagttagt aattatttcc
    16261 caaccacaaa gccaggacat agatttcccc acttctggct aaactcacaa ctaagtagtc
    16321 aagctctctt atcctataaa aattttacac tcattctagg ttcaaaagaa aacaaatggg
    16381 gctctgctct aaaaaaaata aatctagata ttccaatcac agtttccttt ttgagcgaat
    16441 acaatgagaa tctaggagaa ttatacaaaa aagcagaaat tgaatcgaca ggcgctattc
    16501 tagtgcgacc agatggtcag gttgcctatc gaaaacaatt cctctcaaaa ggagaagacc
    16561 cttataaaat actagaatcc atattccaaa caattctttc gtctagacaa gaaaaaataa
    16621 ataaggatgt agccgcatga aagtattttt gattctagcg ataattctac ttttaccaag
    16681 tttaggattc ttttattatc tatttcgacc tctaaggatt gttcccaagc cagcagcttt
    16741 tcaatgcatc ccaatacaag atggaaagct agaggcattt actccaaatc caaatcatat
    16801 ctttggaatg ggtcttgttt atagtaaaca tattgaggaa actgcttcaa acttttcaat
    16861 agtagagcct cccccgattt ttatcaaaga caaacaatca ttagcctctc acaattcaga
    16921 ggtaaaaacc cctaattctt tggatctatt tcaagcatta ttaaaatatg aaccttctct
    16981 agaatcagaa gaaggcttag tgaataacaa ggatttgcct ccacttctag actatgaagt
    17041 agaacttgga tttgtattac ttgcggatat aaaaaaagaa gatttataca aaaaagaatt
    17101 tattccaccc attggatttt ttctcgcaaa tgatatttct gctagaacaa tagcagtgtt
    17161 tggagaagga aggcaagaca aagcagagta ttggggaatt tctaaaagtt ttccgaattt
    17221 tttaccagta tcttcgcagg tttggattcc aagtcatgga cttccgaatg ggattccttg
    17281 tattcgaatt caaacctttg taaatggaat cgaacgacaa aatgaagtta cggttaatat
    17341 gatatatacc cccaaagaaa tgctccaatt tatttctgag aaatataaga acatacctct
    17401 taaaaaaaat gatatggtaa ttatgggaac tccaggaggt gtagccatgt ccactcctag
    17461 atggatggtt agaatctctg atctaatcgg tctcaatcga tttcaaaaat taaaaatagt
    17521 cttaagaaag gatagaacaa attttttaaa gtctggagac aaagttttaa ttcgaggcga
    17581 aggctttgga gaagtagaaa atacaatagt taattaactg acattatgca aaagatagaa
    17641 atttatctgg ataataaaaa agattattat attacgtttt tcgtaaccta tcaggctgct
    17701 aagttacttt tatcaagaga atataatttt aa
//
LOCUS       DAOQOS010000114        17376 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_1644_length_17376_cov_6.915652, whole genome shotgun sequence.
ACCESSION   DAOQOS010000114 DAOQOS010000000
VERSION     DAOQOS010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 17376)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17376)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17376
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1644_length_17376_cov_6.915652"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(123..965)
                     /locus_tag="PLF66_19890"
     CDS             complement(123..965)
                     /locus_tag="PLF66_19890"
                     /inference="COORDINATES: protein motif:HMM:NF033170.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LIC_13355 family lipoprotein"
                     /protein_id="HMZ66362.1"
                     /translation="MYQTIIVLASLISLINCSTNKSSKGNSLLAAALLLSNSRASTSV
                     SSQVTIPADTADTVTSAPGDTGSGFQDKTKAINGVRGGGFSTGSTDVFSLTSTGSSAS
                     IVLEWKGKRVLNGSGIDFIVFENPFQYNGNASSIFMEPIIVEVSQDNISYCGFSPDYT
                     FSPETTYSTNPTYWKRFAGITPVLYNVETNPLSGNDLYDITKTGGDGFDLDNLSGAND
                     FNIGCSTTLRDKIKSEGFVYLRLISATARTNADTGSNFLQDTGAFGGGPDIDGVIARY
                     RATR"
     gene            complement(1235..1822)
                     /locus_tag="PLF66_19895"
     CDS             complement(1235..1822)
                     /locus_tag="PLF66_19895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66363.1"
                     /translation="MFNKKSLVVTLCYVALLVGNCQNVSKTDEADKNNALLATIVQTQ
                     NLNAANLTIATLNNSQFILNGSWNSFTGNGTTSNTIVTISAKSGSNGVILNDSSGFGG
                     YSSCAIVTAFDNSAGYYVSQNPENNGGCFAGDTNKGKFFKTIFFAEGSKYWTCTINTP
                     VATSALAVAVTDNTTKTSPGTTGCGGFSWSRLEKR"
     regulatory      complement(2141..2269)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(2265..3446)
                     /gene="purT"
                     /locus_tag="PLF66_19900"
     CDS             complement(2265..3446)
                     /gene="purT"
                     /locus_tag="PLF66_19900"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012389673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HMZ66364.1"
                     /translation="MVNIGTPKSKTATKLLLLGSGELGKEVTIEAQRYGIHVMAVDKY
                     PDAPAMHVAHEFRIINMLDANELRKVIHEFKPDYIVPEIEAIATDLLIELEKEGFNVV
                     PTAKAVKLTMNREGIRKYASEELGVKTSKYFFAANKEEFYKVVKEIGLPCVVKPVMSS
                     SGKGQSVIKDKMEIEEAWEYAQKGGRAGGGKVIVESFIDFDYEITLLTIRHAGGTTFL
                     DPIGHLQINGDYVESWQPQPMSDVALKEAQRIAKVVTDGLGGYGLFGAEFFIKGDTVY
                     FSEISPRPHDTGMVTLISQNLSEFALHVRAILGLPIPNIRHYGFAASCAILTEGNSDE
                     PIYENINEALSEPDTALRLFGKPEVKGRRRMGVALALGDSLEDARRKAVKVADAITIK
                     L"
     gene            3643..3822
                     /locus_tag="PLF66_19905"
     CDS             3643..3822
                     /locus_tag="PLF66_19905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66365.1"
                     /translation="MIQGNKNSLNKSMMALILHTLEESEKEAIEPEQGNESSKQKENN
                     PSPIANEKFSFIKIE"
     gene            4268..6727
                     /locus_tag="PLF66_19910"
     CDS             4268..6727
                     /locus_tag="PLF66_19910"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HMZ66366.1"
                     /translation="MKKILLLVLFLLTYSQIYSQSEKKFKTQIIQSLEEITSLDSSKE
                     SSWFVSTTDLNPDEFSRKYLENGENAIEWITHIVPSRFVKTNYNKPDIHKIWLAKSVT
                     LPNVIQTNSVGIRLGTISDRDRTYFNGVLIGATGEFGQNYPSAYDKVRIYEIPTSIIR
                     KGAENLILVEAEEYFYDTMGIDQDKTEIGPVRLIEKSFINFEYVKLVLLMIYLVVGIY
                     YLFLFARRRKEKENFIFGIFTVMLVIYQIMRNQLRNEFNFDFVLMKKSEYIVIATIVP
                     VFTHFIRHLFNFEWKLPQKICDGILFTVFIYEMVFTNIRHYNIINTMIVQPIWIIYMI
                     FIFYFLIKRMREKNHDAIFIFSGVIVMFIAVIVDILSTRGVFVFPKIMGYAFFFFISS
                     LAIILANKSVRLHEEVEELNSNLEMKVAQRTEELSQSLEHVKKLKMSQDGDYFLTSLL
                     IKPLGKNRAEKNQVLIDFIVKQKKTFEFKNKMSDLGGDLCRAESLVLRNRNMTVFFNA
                     DAMGKSMQGAGGAIVMGSVFDSILERTKLSQTVQEEYPEKWLKNAFIELHKVFESFQG
                     SMLVSIVIGLIDNKTGLMYFINAEHPVSIIYRDGKASFIDEDFIFRKLGTEVAQSYIY
                     VKTFQMKDGDIVINGSDGRDDIIILDEKTGEKSLNYDHTLFLGTVEKANGNIHTIVDL
                     LHKQGEITDDLSLMSIQYTSQEAQFDSETTRDKIKTLMQNKNFSEAAVLAEDYLLLNP
                     SDTESIFLLSYAYKKLQNYKDAIKAGERVRLREPSHVRNLVNLAHCYFRMDDIDRAKY
                     LANLIPEGRREKDFAKIFLIE"
     gene            complement(6946..7350)
                     /locus_tag="PLF66_19915"
     CDS             complement(6946..7350)
                     /locus_tag="PLF66_19915"
                     /inference="COORDINATES: protein motif:HMM:NF015180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerC domain-containing protein"
                     /protein_id="HMZ66367.1"
                     /translation="MLTSLRKNFENLDSVTAIGIFLSVACAIHCMALPILLFIAPITG
                     FVFFENEILEFFILGISVLITGFNLYSNFKYHKSIQIILLFSLAVILIVFGNFSPEYL
                     KPWMDAFGSFCLSTTLFWNLRLTHSHSEHCSH"
     gene            7561..8655
                     /locus_tag="PLF66_19920"
     CDS             7561..8655
                     /locus_tag="PLF66_19920"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HMZ66368.1"
                     /translation="MHILKKILITLSAILIVAILGVTALVYSLTYHPSDLQQEKVTCK
                     DNAPTLQPGQTIKVLNWNVQFMAGKKYVFFFDVPSGNGPHERPEPSEITKTLLEVARI
                     IKDEKPDIILLQEMDEDAKRTDNQDQLVELMKLLPNEYTCSSSAFYWRNKYLPHPRVK
                     GSTGLKLSTISKYKIESSVRHALAIIPADPITKQFGLKRAVLESKMPIGNSKYISAMN
                     THLDAFAHGYDTMQRQIEYLKNLFNQRNAEKVDWFIGGDFNLLPPNFDLSTLYKTHQE
                     FYSPKSEISVLFDSFKSAVPLEAMGGKDKESYYTHIANDPDLQGKADRTIDYIFHSDG
                     LKTTSYRVRQKDTREISDHLPLIVEFQLPN"
     gene            8660..9661
                     /locus_tag="PLF66_19925"
     CDS             8660..9661
                     /locus_tag="PLF66_19925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008594893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I mannose-6-phosphate isomerase"
                     /protein_id="HMZ66369.1"
                     /translation="MQKILRFKPIYKDKIWGGRKMESVLSRTIPDGNIGESWEVSDYG
                     SDLSIITNDEFAGRSFREVYKQNKEKILGNVFKDNEDFPLLVKIIDAKDKLSVQVHPD
                     DEYANTKDPESSGKKEAWYILETDQNAEIVCGFSESLNREKYESLIQSNRAESCLQTF
                     HSKSGDAFMINPGTVHAIGAGNLLLEVQQSSDSTYRVYDYGRLGDDGKPRKLHLQKAL
                     DVLNFQKSNGQEKISPILLDWKYGKRKLLAANDKFRFEILEFSQTSKLPRFCKENVFL
                     IIHILSGLCSIEREEFKQGDTFLITAEAFEKEITISSLSSETKIAIMSVGSDWVTYL"
     gene            9734..10102
                     /locus_tag="PLF66_19930"
     CDS             9734..10102
                     /locus_tag="PLF66_19930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004784589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HMZ66370.1"
                     /translation="MIIVEGIDHVTIAVTDLAASAKFYADLFDFEILDDSNSKYTIMT
                     LDPIKVKLMQVDKVENQLSGSLTPVLSFILDVDDFTEAISEIEEKGIKIVKGPESAGK
                     GESLIFKDPDNNLIELFYQS"
     gene            10182..11294
                     /gene="prfB"
                     /locus_tag="PLF66_19935"
     CDS             10182..11294
                     /gene="prfB"
                     /locus_tag="PLF66_19935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002972244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HMZ66371.1"
                     /translation="MEIRPAKELLKVSKELQENFQTRWKSLNLQTDYDRLASYKEQAK
                     DPKFWDNPDKAQEISKTQNALEKKLEPWLAIKQEILDFPDLIEMTLEESSDESTVLSL
                     NNDYNRLLEEFTKLELLGSLSGEEDYNNAFFNIHPGAGGTESQDWAEILFRMYTRYFE
                     KKGYRVDIIDYQEGEGAGLKNVTLHVQGEYAFGYLKGENGIHRLVRISPFDANKRRHT
                     SFASVHVSPELEDDIDIVIEEKDLRVDTYRSSGAGGQHVNKTDSAVRMTHIPTGIVVQ
                     CQNERSQIKNRATAMKMLKAKLYELEKKKSEEENQKKSGEKKDIAWGSQIRSYVFHPY
                     NLIKDHRTDHETGNVQPVMDGELDDFVMAYLKYISS"
     gene            complement(11291..12787)
                     /locus_tag="PLF66_19940"
     CDS             complement(11291..12787)
                     /locus_tag="PLF66_19940"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HMZ66372.1"
                     /translation="MKYQFQYLIKVFLFLCLFGLESSELEKSKKEKMKVRSEIVKVAL
                     NSKADNLYKPLTLEDLISNINENFSLLLAAFQDIAIADGELLSARGAFDGSIKANGTT
                     APTGFYSHNRMDMLIDQPTTYNGMSFFGGYRLGRGSFAPYDGKLATNSLGEIRAGAKI
                     PLWRDRPIDKNRAAIKQAEIGVKIADLSVAQQKIDIIRNATLRYWDWIAAARKYIIAK
                     NLYDIALEREKQILKRVKAGDIPPIEQVDNERVLLQRESFLLGSAQALEIASNELSIY
                     LTDANGKARSPRIEEIPENIFSLFEDIKDNNLEKEIEQALIRRPELERFKAQRDQNQV
                     DQELARNQMSPGVDIVIAASQDLGTGSVTRSRPEVEAAIVLNVPLRTRTQQGKLDSAS
                     AKNEKLSKQEQFFRDRISADIRNTFTTVNVTRQRAVLAQKEFLLAKKLEEAERTRYVM
                     GEGTLLVVNIREQTTGEAAIREVDALADHHRAVANFKAAIADVLKKKD"
     gene            complement(12784..14127)
                     /locus_tag="PLF66_19945"
     CDS             complement(12784..14127)
                     /locus_tag="PLF66_19945"
                     /inference="COORDINATES: protein motif:HMM:NF024829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HMZ66373.1"
                     /translation="MSKSEYSKNNIQEENLSLDRLISKSYSLSRTASVIFTCFCICIL
                     LMFILPWQQTVFGTGRVVAYAPLNRQQFIEAPIDGRIVHWHVMEGTQVKKGDNIVEIS
                     DNDPQFLERLQEEKAAIESRVIAIQARAESFKARIKSLEASMDSGVSAAKSRTRMSGD
                     RVDAAEHAVEAAEAVYKTATLNLNRQKSLASSGLASARAVEMAEVESARALTDLNRAK
                     ASLSAAKSEAVAIQADQFKVGTDATASIADARASYSAALAELANAKAELPRISARLAR
                     QHSQSVTAPRDGTIMRLIVSQDGEMVKSGEPLAVLIPESADRAAEIWVDGNDIPLIVE
                     EAKAQVQFQGLPALQFSGWPGISAGTFSARVILVDNTDNGTGKFRVLVKPVSPSDWPE
                     QGFLRQGVRAHAWIFLNEVSIAYELWRKFNGFPPDLPYTSNLTDKAKSQDKKDSK"
     gene            complement(14120..16252)
                     /locus_tag="PLF66_19950"
     CDS             complement(14120..16252)
                     /locus_tag="PLF66_19950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMZ66374.1"
                     /translation="MNQKIQSESFNMYALRILEKFAESDGVEFDSVKTLTEIESYTRK
                     KKLDQTSLNLSDLVSIAEKLKIYLNIEISHWNDIIQKVSPSSPVAIYLKEEGWLFIHG
                     VFGPLVRCTRVSDSSVFWTLKRNIVNKIQSSQDNGDYPQWLILKKKFSVLGKDYHSSH
                     DYLSSIFRFIRIEKKDIWAIAIYSVAIGLFSLIIPMAVQSLVNILNFGTLFQPVLILT
                     LIVIIALGFVGYLNVLQAYVAELIQQRLFLRLAANVVTLLPRVNYASMKKYYGSEIPN
                     YFLDISIIQKSATILLTSGLGIFLQTIIGLLVLVLYHPIFILFNVIILGFVIGIIFYL
                     IGSVGIETSIKESKSKHKLASWIQEMNFHPMMLRSANSREYATLRSENLAREYLTARR
                     KHFKALIRQMIGFVSLQALGSGLLLGIGGWLVIKGQITLGQLVASEIIVSKILDNFGK
                     LTKYLENYYDLCASVDKVNHLLDFETEVEGDFTPEIQKDTPISVHVENLNFNTGDIWI
                     EKFTANKGEIIQIQDASKVNAMKFMEILNGLNVIESGKLELDSYNIKDINLSELRNHV
                     LLLRDLDIFSGTISENLTLGKWSYDPKEIRMALAKVGLDGKVDQLPDGIATDILRNGY
                     PFRKEELALFCIARIFITKPSLILIDNILIYLSKESLQKVLGLLKECKDFATIIIASN
                     LPEIQKLSDRTYIFKDQNLVESRKEKNV"
     gene            16506..16985
                     /locus_tag="PLF66_19955"
     CDS             16506..16985
                     /locus_tag="PLF66_19955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002189022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4395 domain-containing protein"
                     /protein_id="HMZ66375.1"
                     /translation="MIKIGNFPEIVNENAARIVALFVVIISLSAIYFESIFLASLLFY
                     GFTARVLYGPKFEPFARLTLDIIIPFLKIGNKPTPGIPKRFAQFIGFLFSLTALILLL
                     SQNIFEFKITLGILSFFAFLESFFGFCAGCFIFSYLMKWGFVPNDVCERCSNINYHI"
     gene            complement(16982..>17376)
                     /locus_tag="PLF66_19960"
     CDS             complement(16982..>17376)
                     /locus_tag="PLF66_19960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66376.1"
                     /translation="ILTGLMSLGTSALAKGLPLLQTKAEFENRKIKIAETLKKLAKVD
                     YGVSYQKWFDWAIYNGYTVNGVNLIHYLFSGYPEKRNLAIDSAIKSLGYKETKEYFDL
                     YPNKTQTEIVLMIAEELLKTGIQQDEKY"
BASE COUNT         5843 a         3168 c         2770 g         5595 t
ORIGIN      
        1 tttattaaaa tctattctgt aaaccctttt tcttatttcg caacagactg ataatacatt
       61 ccatcgagtg atagatttgg atacttccgt ggttcgtctt tattttcctc tgattccatt
      121 tttcacctag ttgctctata tctagcgatt actccatcaa tatccggacc acccccaaac
      181 gcacctgtat cttgtaggaa atttgatcct gtatccgcat tggttcttgc cgttgcagag
      241 attaatcgta agtaaacaaa cccctcagac ttaattttgt cgcgtaacgt cgtagaacaa
      301 ccaatattaa aatcatttgc tccagataga ttatctaaat caaaaccatc tccaccagtt
      361 tttgttatat cataaagatc atttcctgaa agtggatttg tttcgacatt atagagaaca
      421 ggtgttatcc ccgcaaatct tttccagtag gtaggatttg tgctgtaagt tgtttcaggg
      481 gaaaaagtat aatcaggaga gaacccgcaa taacttatat tgtcttgact gacttccaca
      541 ataatcggtt ccataaaaat agaagaagca tttccattat actgaaatgg attttcaaaa
      601 acaataaaat caattccact tccgtttaac actcgtttac ctttccattc taaaacaata
      661 gaagcagaag aaccagttga ggttaaagaa aatacgtcag tcgaaccagt agagaatcca
      721 ccacctctca ctccattgat cgcctttgtc ttgtcttgaa accctgatcc tgtatctcca
      781 ggcgcagatg taaccgtatc tgctgtatca gctggaatcg ttacctgaga tgagactgag
      841 gtcgatgctc tagagtttga aaggagcaat gcagcggcta acaatgaatt tcctttactc
      901 gacttatttg ttgagcaatt tattaaacta atgagacttg ctaaaacgat tatggtttga
      961 tacattttaa ttctccttgc taaaagcaat agtagagata gcgattaatg cgggtgactg
     1021 tgtaaaagat taaaaaaatc ttaccacgaa ttgcacgaag atcgcgaaga tgaaaatttc
     1081 ctaagaaatt tacaattatt cttcgtaacc ttcgtgcact tcatgatgaa gctcttgctt
     1141 ttgcagagtc ccctaagcga aaagctcgat cggtgctttc ttaaatgtta agacttatgc
     1201 acattaaaaa atttaccgaa tagtcctaaa ctcattatct cttttctaat ctactccacg
     1261 aaaacccacc gcatcctgtc gttcctggac ttgtttttgt tgtattatcc gttacagcaa
     1321 cagctaacgc tgatgtcgca acgggagtat taattgtgca tgtccagtat ttacttcctt
     1381 ctgcaaagaa tatggtttta aagaactttc ctttatttgt atcaccagca aaacatccac
     1441 cattattctc tggattctga ctaacataat aacccgcaga attatcaaag gctgttacaa
     1501 tagcgcagga gctataacca ccaaaaccag aagaatcatt taatataact ccattagaac
     1561 ccgatttagc agaaattgta acgatagtat tgctagttgt tccatttcca gtaaaactat
     1621 tccaactacc attcaaaata aactgactat tatttaatgt tgcaatagtt aaattcgcag
     1681 catttagatt ctgtgtttgc acaattgtag cgagtaacgc attattttta tcagcttcat
     1741 ctgtctttga aacattttga caatttccaa ctaaaagcgc cacataacaa agagtaacca
     1801 ctaaactttt tttattaaac atatttttct ccaagaaaat ttttcctgaa ggagttatac
     1861 gaacctctac gcaagaaaga gtattttaga taagttttta atacataaaa acaaaacaga
     1921 tttcacacaa cgcctattaa gtaacgccag ttcgacgtaa caaaaaattt aaaagatgac
     1981 caccgatgaa cacggataaa ataataataa ttcaaaccat ccgcgcttat ctgtgttcat
     2041 ccgtgtctat ccgtggttaa atattcttat atcgaatgca cgttaagctg tattaaaatc
     2101 tgaatttcta aaaagaatct ttcaatgaaa ctttcctttc cacgagaaag taacacaaag
     2161 aggagtatct ggctttacag ttgcgggaca gcgagggact ttcaccctac ttcctccatt
     2221 cggatgtttc catatttaca atagagtttc ttaggtcaag tcaattacaa tttgattgta
     2281 attgcgtcag caaccttcac agcttttctt cttgcatctt ctaaagaatc tccaagtgcg
     2341 agagctacac ccattcttct tctacccttt acttctggtt taccaaatag acgtagagct
     2401 gtgtctggtt cagaaagagc ttcatttata ttttcataaa taggttcgtc agaatttcct
     2461 tctgttaata ttgcacatga cgcagcaaat ccatagtgtc gtatatttgg aattggaagt
     2521 cctagtatag cacgaacatg aagcgcaaat tcagaaaggt tttgagaaat cagcgttacc
     2581 attccagtat catgtggtct tggagaaatc tcactaaaat aaactgtgtc gcctttaata
     2641 aaaaattcag caccaaacag tccgtaacca ccaagtccat cggtcacaac ttttgcgatt
     2701 ctttgtgctt cttttaatgc gacatccgac ataggttgag gttgccaaga ctcaacatag
     2761 tcaccattga tttgtaaatg accaatcgga tctaagaaag ttgtcccgcc agcatgtctg
     2821 attgtaagaa gagtaatttc gtaatcaaaa tcaataaaac tttccacaat cacctttcca
     2881 ccaccagcac gccctccttt ttgagcatac tcccaagcct cttctatttc cattttatct
     2941 ttgatgacac tttgcccttt gccagaagaa ctcataactg gtttaacaac acaaggcaga
     3001 ccgatttctt ttaccacttt gtaaaattct tctttgtttg cggcgaagaa atacttagaa
     3061 gttttaactc ctaactcttc cgatgcatat tttcgaattc cttctcgatt catagtaagc
     3121 ttaacagcct ttgctgtcgg aactacattg aaaccttctt tctccaattc tatcagtaga
     3181 tctgttgcaa tagcttcaat ttctgggaca atataatcag gtttaaactc atggattacc
     3241 tttcttaact catttgcatc tagcatattg ataattctaa attcatgggc tacatgcatt
     3301 gctggcgcgt ctgggtattt gtctacagcc ataacatgaa ttccatatct ctgtgcctct
     3361 atagttactt cctttcctaa ttctccggaa cctaataata gaagctttgt tgctgttttt
     3421 gattttggag ttccaatgtt taccatactt atatcctctc gattcaataa agaagctaaa
     3481 tctttaactc caaaaaatat tatctttata ataagtcatt cctgttttta aatgaaccaa
     3541 ttggacagga aaattgagcc tttactaaat tctaaaaaaa agaaataaaa gctttacaaa
     3601 tattgttgat tttgattctc attatccata aaggagtaaa agatgattca aggcaataaa
     3661 aactctctaa ataaatcaat gatggcatta atacttcaca ctctagaaga atctgaaaag
     3721 gaagcgattg agccagagca aggaaatgaa tcttcaaagc aaaaagaaaa taatccatct
     3781 cccattgcga atgaaaaatt cagctttatt aaaattgagt aaattggaaa tcctaagaag
     3841 aaaaaggaaa ctaattttat aggatttttt taaattccct aaaaaaacaa attcgatcga
     3901 aaagattaag aatgattgaa atagtatttt tataaaaata ctatttcaat ataagactct
     3961 gaatcttata aataacttat gtcagttaag agctatctat ccttagtgac tttgttttaa
     4021 acaaaacgat tctaactttt taaaattgag attatagcat tggcaaaaaa gttatataag
     4081 cgaacaaatc ttatatttct tccgctttca caggagctta atttttaagt tccaaaaatg
     4141 tgttcatctg catccaattt ttctaattaa ttctgttaat tctgaattag agtttgacca
     4201 atttaaaaaa acatgaaaac ttacaatttt tcccaatatc acagataaaa atcaggattg
     4261 taatttcatg aaaaaaatat tgctacttgt tttatttctt cttacttatt ctcaaattta
     4321 ttctcagtca gaaaaaaaat ttaaaacaca aatcattcag tctttagagg aaattacaag
     4381 tcttgattct agtaaagaat ctagttggtt tgtttccaca accgatttaa atccagatga
     4441 gttcagccgc aagtatttag aaaatgggga aaatgcaatt gagtggataa cacatattgt
     4501 cccctctcgt tttgttaaga ctaattataa caaacccgac attcataaaa tctggcttgc
     4561 taaatcggta accctaccga atgtaattca aacaaattct gttgggattc gtcttggaac
     4621 gataagtgat agagatagga cttacttcaa tggagttcta attggagcaa ctggtgagtt
     4681 tggacaaaat tatccatctg cctacgataa agttcgtatt tacgaaattc ctacaagtat
     4741 aattagaaaa ggagctgaaa atctaatctt agttgaagca gaagaatatt tttatgatac
     4801 tatgggaatc gaccaagaca aaacagaaat tggacctgtc cgtttaatcg aaaaaagttt
     4861 tatcaacttt gaatacgtca agcttgttct tttaatgata tacttagtcg ttggaattta
     4921 ttatctattt ctatttgcga gaagaagaaa agaaaaagaa aattttattt ttggtatttt
     4981 cactgtaatg cttgtgattt atcaaattat gcgtaaccaa ttaagaaacg aatttaactt
     5041 tgactttgtc ctaatgaaaa aaagcgaata catagttatt gcaactattg tccctgtctt
     5101 tactcatttc attcgacatt tatttaactt tgaatggaaa cttcctcaga aaatatgcga
     5161 tggaattcta tttactgttt ttatttacga aatggttttt acaaacattc gacactataa
     5221 catcatcaat acaatgatcg ttcaacctat ttggatcatc tacatgattt ttatttttta
     5281 ttttttaata aaaagaatga gagagaaaaa ccatgatgct atttttattt tttctggcgt
     5341 aattgttatg ttcattgctg tcattgtgga tattttaagc acacgaggag tttttgtttt
     5401 tcctaaaatt atgggttatg catttttctt ttttatttcc agccttgcca tcatactcgc
     5461 aaataagtct gttcgattgc atgaggaagt ggaagaactg aattccaacc tagaaatgaa
     5521 agtcgcacaa cgcacggaag agttaagcca aagcttagag catgttaaaa aattaaaaat
     5581 gtcccaagat ggagactatt tcctaacatc cttattaatc aaaccgctag gaaaaaatcg
     5641 agcagagaaa aatcaagtgc tcattgattt tatagtaaaa caaaaaaaga cttttgaatt
     5701 caaaaataaa atgtccgatc taggtggtga tttgtgcagg gcagaaagtc tagtattgcg
     5761 aaatagaaat atgactgttt ttttcaatgc agatgcaatg ggcaaatcta tgcaaggtgc
     5821 aggtggtgca attgtaatgg gatctgtttt tgattccata ttagaaagaa caaaattaag
     5881 ccaaacggtt caagaggaat atccagaaaa gtggttaaag aatgcattta tcgaactaca
     5941 caaagtcttt gaaagctttc agggttctat gcttgtatcc atagtaatcg ggcttatcga
     6001 taacaagact ggacttatgt attttataaa cgcagaacat cctgttagta tcatttatag
     6061 agatgggaaa gctagtttta ttgatgaaga tttcattttt agaaagcttg gaacagaagt
     6121 cgctcaaagt tacatctatg taaaaacatt tcaaatgaaa gatggagata tagtaattaa
     6181 cggttctgat ggaagagatg atataataat tttagatgaa aaaacgggag aaaaaagcct
     6241 aaactatgat cataccttat tcctaggaac tgtcgaaaaa gcaaatggta acatacatac
     6301 gatagtagac ctacttcata aacaaggaga aattactgac gatttatctc tcatgagtat
     6361 tcagtatact agccaagaag cacaatttga tagtgaaaca acccgagata aaattaaaac
     6421 tttaatgcag aataagaatt tctctgaagc agctgtctta gcagaagact atctacttct
     6481 aaatccatca gatactgaaa gtattttttt actttcctat gcctacaaaa aattacaaaa
     6541 ctacaaagat gcaattaaag cgggagagag agttcgtcta cgagagccat ctcatgtgcg
     6601 aaatttagtc aatcttgccc attgttattt tagaatggat gacatagata gagcaaagta
     6661 tttagcgaat ctaattccag aaggaagacg cgaaaaagat tttgctaaaa ttttcctaat
     6721 tgaataacaa tgataaataa tcaactgatt ctttgcaatt tcggacgtat cgattttcat
     6781 agaagatcca gaatcagttg attttgtttt tgttacaata tagattgaat cgaaaataaa
     6841 tatttgaaat tcaaattaaa atctcacctt acacaaaaga tagaagttaa agatcacttg
     6901 tctatttagt ataagttgtg ttagaatcta aatttagaat aaggattaat gactacaatg
     6961 ttcgctgtgt gagtgcgtta aacgcaagtt ccaaaacaag gtagtagata aacaaaagga
     7021 gccaaacgca tccatccaag gctttaaata ttcaggagaa aaattaccaa aaacaattaa
     7081 aataaccgca agtgagaata ataaaataat ctgtatactt ttatgatatt taaaattgga
     7141 ataaaggtta aaaccagtta tcaaaacaga aatacctaga ataaagaatt ctaagatctc
     7201 attttcaaaa aatacaaatc cggtaattgg agcaataaac agtaaaattg gaagagccat
     7261 acaatggatt gcacaagcga cggaaagaaa aataccaata gcagtgactg aatctaaatt
     7321 ctcaaaattt tttcgtaaac tagttagcat ttttattcct ctttatgcaa catagttgca
     7381 gtaagtttta ttgtcaaatt ctttaattca aacttaagca atctggaatt tttccatttg
     7441 ttaaaattgt tggagaacta tgtcttactc ctttcaatca aaaaaagaaa atccaaatag
     7501 aatttcacag tttacaaaaa cattattttt tgaagttctt gttcttacgc gaggaaatac
     7561 atgcatatat taaaaaagat actgattact ctatctgcca tattaatagt tgctatacta
     7621 ggtgtaacag ctttggttta tagtttaact tatcatccat ctgatctaca acaagaaaaa
     7681 gttacctgta aagacaatgc acctactcta cagccgggac aaacaataaa agttttaaac
     7741 tggaatgttc aatttatggc gggcaaaaag tatgttttct tctttgacgt tccaagtggc
     7801 aacggaccgc acgaaagacc tgagccttca gaaattacaa aaactctttt ggaagtagca
     7861 agaattataa aagatgaaaa accagatatc atacttttac aagaaatgga tgaggatgca
     7921 aaaagaacag acaaccaaga tcaattagtt gagctaatga aattactacc aaacgaatac
     7981 acatgcagtt cctccgcatt ctattggaga aataaatatc tacctcatcc aagggttaaa
     8041 ggctcaacag gattaaaact ttctaccatc agtaagtata aaatcgaatc atccgtgcgt
     8101 catgcgctag ctatcattcc ggctgatcct attacaaaac aatttggttt aaagagagca
     8161 gtcctagaat caaagatgcc gataggcaat tctaaataca tttctgccat gaacacacac
     8221 ttggatgcat ttgctcatgg atatgatacg atgcaaagac agattgaata tttaaaaaat
     8281 ctatttaatc aacgaaatgc agaaaaagta gattggttta tcggtggaga ttttaaccta
     8341 ctaccaccta actttgattt atctactctc tataaaacac accaagagtt ttacagcccc
     8401 aagtcggaga tatcggtttt atttgattcc tttaaatctg ctgtgcccct agaagcaatg
     8461 ggcgggaaag acaaagaaag ctattatacc catatagcaa atgatccaga cttacaggga
     8521 aaagcagata gaacgattga ttatattttc cactcggatg gtcttaaaac tacgtcctat
     8581 cgagtcagac aaaaggatac aagagaaatc tctgatcatc ttcctttaat agtagagttt
     8641 caattaccaa attaaaatta tgcaaaaaat tctgagattt aaaccaatat acaaagataa
     8701 aatatgggga ggacgaaaaa tggaatcagt cctctctaga acaatcccag acggaaacat
     8761 tggcgagtca tgggaagtat cagactatgg aagcgattta tcgatcatca caaatgatga
     8821 atttgcaggt agaagctttc gagaagtata caagcaaaac aaagaaaaaa ttcttggaaa
     8881 tgtctttaag gataatgaag attttcccct acttgtaaaa ataattgatg ctaaagataa
     8941 attatctgtc caagttcatc cagatgatga atatgcaaat accaaagatc cagagagttc
     9001 tggaaaaaaa gaagcgtggt atattttaga aacagatcaa aatgctgaaa tcgtttgcgg
     9061 tttctccgaa tcactcaatc gagaaaagta tgagagtcta attcaaagta accgcgctga
     9121 atcatgtttg caaacctttc attcaaaatc cggggatgca tttatgatca accctggaac
     9181 agtgcatgca attggcgcag gaaatctact tttagaagtc caacaatcct cagattctac
     9241 ttatagagtt tatgattacg ggcggttagg tgatgacgga aaaccgagga aacttcacct
     9301 tcaaaaagcc ttggatgttt tgaattttca gaaatcaaat ggacaagaaa aaatttctcc
     9361 tatccttcta gattggaaat acggaaaaag aaaattatta gccgcaaatg ataaatttag
     9421 gttcgaaatt ttagaatttt cccaaacaag taaactccct agattttgca aagaaaatgt
     9481 ttttctaatt attcatatac tttctggttt atgtagtatt gaaagagaag aatttaagca
     9541 gggtgatact tttttaataa ccgcagaagc atttgagaag gaaataacaa tctctagcct
     9601 ctcaagtgaa actaaaatag ccattatgag tgtcggatct gattgggtca cttatttgtg
     9661 atgtaaaaac atttttcaat ggacagcgtg ccattttcaa ccaaactggt aaaaaaacaa
     9721 ataggtttat ggaatgataa tagtagaagg aattgaccac gtaactatag ctgtaacaga
     9781 tttggcagct tcagctaaat tttacgctga tttatttgat tttgaaatcc tcgatgattc
     9841 caattcaaaa tatacaataa tgactcttga tccaatcaaa gttaaattaa tgcaagtaga
     9901 taaagttgaa aatcaactca gtggaagcct cactccagtt ttgagtttca ttttggacgt
     9961 agatgatttc acagaagcaa tttctgaaat cgaagaaaaa ggaataaaaa tcgtcaaagg
    10021 tcctgagagt gcaggaaaag gcgaatctct tatttttaaa gatcctgata ataatttaat
    10081 agaattattc taccaatcct gaattaaaaa acaacttcag atagggggca ttttaaggac
    10141 acatccttga aatgcctttt ttattttcta gaggtgcatc catggaaatt agaccagcga
    10201 aagaattatt aaaagtcagc aaagaactcc aagaaaattt tcagacgaga tggaaatctc
    10261 tcaatctaca aactgactat gatagattag cctcttataa agagcaagca aaggatccaa
    10321 aattttggga taatccagat aaagcccaag aaatatctaa aactcaaaac gctctcgaaa
    10381 agaaattaga accttggctt gcaattaaac aagaaatttt agattttcca gatttaattg
    10441 aaatgacgtt agaggaatca tcggatgaat ctacagtcct ttctttgaac aatgactata
    10501 atagactttt agaggaattt actaagctag aacttctggg atcattaagc ggcgaagaag
    10561 attataataa tgcttttttt aatattcacc ctggtgcagg tggaacagaa agccaagact
    10621 gggcagaaat tttattcaga atgtatacta gatatttcga aaaaaaagga tatcgtgtag
    10681 atattataga ttatcaagaa ggagaaggtg ctggtctaaa aaacgttact ctccacgtcc
    10741 aaggagaata cgcatttgga tatttaaaag gagaaaatgg gatccatcgt ttagttcgta
    10801 tatctccatt tgatgcaaac aaaagaaggc atacttcttt cgcttcagtc catgtctccc
    10861 ctgaattaga ggatgatatc gatatagtca tagaagagaa agatttaaga gtagatacat
    10921 atcgttcttc cggagctggt ggtcagcacg ttaataagac agattcagca gttagaatga
    10981 ctcatattcc aacaggaata gtggtgcaat gccaaaacga gagatctcag attaaaaata
    11041 gagctactgc catgaagatg ttaaaagcta aactctatga attagaaaaa aagaaatcag
    11101 aagaagaaaa tcaaaagaaa tcgggtgaga aaaaagatat agcttggggt tctcaaatca
    11161 gaagttatgt atttcatcct tataacctaa tcaaggatca cagaacagat catgaaacag
    11221 gcaatgttca accggttatg gatggagaat tagacgattt tgtaatggct tatttaaagt
    11281 atatatcaag ttaatctttc ttctttaaca catcagctat agctgcttta aaattggcta
    11341 ctgctctatg atgatcagct aacgcatcaa cttctcttat tgcagcctct ccagttgttt
    11401 gttcccgaat atttacaaca agtaacgttc cctctcccat aacataacga gttctctctg
    11461 cttcctctaa ttttttagct agtaaaaatt ctttttgcgc taaaacagct ctttgacggg
    11521 taacgtttac tgtagtaaat gtatttcgaa tatccgcaga aattcgatct ctaaaaaact
    11581 gctcttgctt agaaagcttt tcattctttg cacttgcaga atccaattta ccttgctgag
    11641 ttctagttct aagaggaaca tttaaaacaa tcgctgcttc gacttcagga cgactacgag
    11701 taacgcttcc tgttcctaag tcttgtgagg cagcaattac tatatcaact ccaggagaca
    11761 tttggtttct tgctaattcc tgatctactt ggttttggtc tctttgtgcc ttaaatcttt
    11821 caagttcagg tcttcgaata agcgcttgtt caatttcttt ttctaggtta ttatctttta
    11881 tatcctcaaa caaagaaaaa atattttcag gaatctcttc tattctggga cttctcgctt
    11941 ttccatttgc atccgttaaa tagatagaaa gttcattaga agcgatctct aaagcctgtg
    12001 ctgatccaag taaaaaagat tccctttgga gtaatactct ttcattatcc acttgctcga
    12061 taggcggtat atctccagct ttcactcgtt tcaaaatctg tttttctcgt tccagtgcga
    12121 tatcatataa atttttcgca attatatatt ttcttgcagc agcaatccaa tcccaatacc
    12181 taagagttgc attgcggata atatcgattt tctgttgtgc aacggataaa tctgcaattt
    12241 ttactcctat ttctgcctgt ttaatagcag ctcgattttt atcaataggt ctgtccctcc
    12301 agagcggaat ttttgcaccg gctcggattt caccaagtga attggttgct aacttaccat
    12361 cataaggagc aaatgatcct ctccctaatc gataacctcc aaaaaaagac ataccattat
    12421 atgttgttgg ctgatcaatt aacatatcca ttctattatg tgaatagaat cctgttggtg
    12481 cagttgttcc attagcttta atacttccat caaaagctcc tcttgcactt agaagctctc
    12541 catctgcaat tgcaatgtct tgaaatgcgg ctaataacaa agaaaagttt tcattgatat
    12601 ttgaaattaa atcttctaga gtgagaggct tatataaatt atccgctttg gaatttaaag
    12661 caacttttac aatttcgctt ctgactttca tcttttcttt tttacttttt tccagctcgg
    12721 aactttctag accaaacaaa caaagaaaca gaaaaacttt gataaggtat tgaaattgat
    12781 atttcatttc gaatcctttt tatcttggct ctttgcttta tcagttagat ttgaagtata
    12841 cggtaaatca ggtgggaagc cattgaactt tctccaaagc tcataagcaa tggaaacttc
    12901 atttaaaaat atccaggcat gcgctcttac tccttgtctt aaaaaaccct gctctggcca
    12961 gtcagaagga gaaacaggtt tcacaagaac tctaaactta ccagttccat tatcagtatt
    13021 atcaactaaa atcacacgtg cagaaaatgt tcccgcagaa attccaggcc aaccactgaa
    13081 ttgaagagca ggtaagcctt gaaattggac ctgtgctttt gcttcttcta cgataagcgg
    13141 aatgtcattt ccatcgaccc aaatttcagc tgccctatca gcactctctg gaatcaatac
    13201 tgcaaggggc tcacctgact ttaccatttc tccatcctga gaaacaatga gcctcataat
    13261 agttccatct ctaggtgctg taacactttg tgaatgttgt cttgcaagtc gtgcagaaat
    13321 tctaggtagc tctgctttag catttgccaa ttcagctaac gctgcagaat aagacgccct
    13381 cgcatccgca atagaagcag ttgcatctgt tcccacctta aactgatcag cttggatagc
    13441 aactgcttct gattttgccg cgctgagaga agcctttgct cgattcaaat ctgtaagcgc
    13501 tcttgctgat tcaacttctg ccatttctac agcacgagcg gaggctaatc ctgaactggc
    13561 taaacttttt tgtctattga ggttcaaagt agctgtttta tataccgcct cagcagcctc
    13621 cactgcatgt tctgctgcat caacacgatc accagacatt ctagttctag acttggccgc
    13681 ggacacacca ctatccatag atgcttctaa tgactttatt cttgctttga aggattctgc
    13741 tcttgcttga atggcaatca cacgggattc aatcgctgct ttttcttctt gtaatctttc
    13801 aaggaattgt ggatcgttat ccgatatttc tacaatattg tccccttttt ttacttgcgt
    13861 cccctccatt acatgccaat ggacgattct tccatcaatg ggtgcctcaa taaattgctg
    13921 cctgttcaaa ggtgcatagg caaccaccct acctgttcca aacacagtct gctgccatgg
    13981 caaaataaac atcaaaagaa tgcagataca gaaacaagtg aaaattaccg aggcggttct
    14041 acttaaagaa taagattttg aaatcaaacg atctaatgat agattctcct cttgaatgtt
    14101 attttttgaa tattccgatt tagacatttt tctctttcct actctctact aagttttgat
    14161 ctttaaaaat ataagttcta tctgaaagtt tttgtatctc aggcaagtta gacgcaatga
    14221 taattgttgc aaaatcttta cactctttta acaatcctag aactttttgg agagactctt
    14281 tagataagta aattaaaata ttatctatta aaataagcga aggtttagtt ataaaaattc
    14341 tagcaatgca aaatagagcc aattcttctt ttcgaaatgg gtatccattt ctaagaatat
    14401 cagttgcaat gccatctggg agctgatcaa cttttccatc taacccaact tttgcgagtg
    14461 ccatcctaat ttcttttgga tcataactcc attttcctaa ggtcagattt tctgatatag
    14521 ttccactaaa tatatccaag tctcttagaa gtaaaacatg atttcttaat tctgataaat
    14581 ttatatcttt aatattataa gagtctaact ctagtttgcc gctttcaatc acatttaaac
    14641 cattcagaat ttccatgaac ttcattgcat taactttaga tgcatcttgt atttgaatga
    14701 tttctccttt gtttgcagta aatttctcaa tccatatatc gccagtatta aaattcaaat
    14761 tctcaacatg aactgaaatt ggagtatcct tttgtatctc cggagtaaaa tcaccttcta
    14821 cttccgtttc aaagtcaagt aaatgattta ctttgtcaac tgacgcacac aaatcataat
    14881 aattttctaa atactttgtt aattttccaa aattatccaa gattttagaa acaataatct
    14941 ctgaggcaac taattgacct aaagtaattt gccctttaat aacaagccaa ccgccaatgc
    15001 ctaagagtaa accacttccc aatgcttgca aggatacgaa tcctatcatc tggcgaatca
    15061 atgctttaaa atgttttctt cttgcggtta aatattctcg agctaaattc tcagatctta
    15121 gagttgcata ttctcttgaa tttgcagatc taagcatcat tggatgaaag ttcatttctt
    15181 gaatccaaga agctaattta tgtttgcttt tggactcctt tatactcgtt tcaataccaa
    15241 cagatcctat taaataaaag attattccga ttacaaatcc caaaataatt acattaaata
    15301 aaataaaaat tggatggtat aaaaccaaaa ccaataagcc tataatagtt tgtaaaaaaa
    15361 ttcccagacc actagtgagt aatatagtag cacttttttg gataatcgaa atatctaaaa
    15421 aataatttgg aatttctgat ccatagtatt ttttcatact agcataattg actcttggca
    15481 ataaagttac aacgttagcc gccaatctca gaaatagcct ctgctgaata agctctgcta
    15541 cgtatgcttg caaaacattt aaatacccca caaatccaag agcaataatt actataagtg
    15601 ttaaaattaa tactggctga aataacgttc caaagtttaa aatattcact agagattgaa
    15661 cagccattgg aataatcagt gaaaacaatc caatcgcaac agagtaaatt gcgatcgccc
    15721 aaatatcttt cttttcaatc ctgataaatc taaaaatgga tgataagtaa tcatgactag
    15781 aatgataatc ctttcctaga acgctaaatt ttttctttag tataagccat tgcggataat
    15841 ctccattatc ctgactagat tgaattttgt ttacaatgtt tctttttaga gtccaaaaaa
    15901 cgcttgagtc acttactctc gtgcatcgaa ccaaaggtcc gaagacccca tgtataaaaa
    15961 gccagccttc ttcttttaga taaatagcca ctggtgaaga aggagaaact ttttgaatta
    16021 tatcattcca atgggaaatt tctatattta agtaaatctt gagcttttct gcaatgctaa
    16081 ctaaatcaga taggtttaaa ctagtttggt ctaatttttt ttttcgtgtg taggactcta
    16141 tctctgtaag agttttgact gaatcaaatt caactccatc cgattctgca aatttttcca
    16201 aaatgcgcaa cgcatacata ttaaatgatt cactctgtat tttctgattc atccataatc
    16261 cttttctaac agtctttaga cacctgaact acatttcatg taaaaaaata tacttgggac
    16321 ataaattgat tttattctta accaatattc aattccagag gataaaaagg tttttcctta
    16381 aaactagtta gtctttcaac cttcatctag tttttttctt gtctcagaga aggcattcat
    16441 gaatcatgta ttatattaga tttttttccg ttatattata caatttttaa tttgtaggaa
    16501 ctattatgat caaaattggt aactttcctg aaatcgtaaa tgaaaatgca gctcgcattg
    16561 tagcactctt tgtagtgata atcagcctat cagctattta ctttgaatcc atttttttag
    16621 cttccttact tttttacggg tttacagcaa gagtcttata tggaccaaaa ttcgaaccat
    16681 ttgctagact tacattagat ataattattc ccttcttaaa aatcggaaac aaaccaactc
    16741 ccggaatacc aaaaagattt gctcaattta taggatttct ttttagccta acagcgctta
    16801 tcctcttact cagccaaaat atattcgaat ttaaaatcac acttggaata ctttcttttt
    16861 ttgcattcct tgaatctttc ttcggatttt gtgcaggttg cttcatcttc tcttatctaa
    16921 tgaaatgggg atttgttccc aatgatgtct gcgaaagatg tagcaatatc aattatcaca
    16981 tttagtattt ttcatcttgc tgaattcctg tttttaatag ttcttcagca atcattaaaa
    17041 cgatttcagt ctgtgtttta ttcggatata aatcaaaata ttcttttgtt tctttatatc
    17101 cgagtgattt aattgcacta tcaatcgcaa gatttctttt ttccgggtaa ccagaaaaca
    17161 aatagtgaat tagattgact ccattgaccg tatatccatt gtaaattgcc caatcaaacc
    17221 atttttgata agaaactcca taatccactt ttgctaattt ctttaatgtt tcggctatct
    17281 ttatttttct attttcaaat tctgcttttg tttgtagaag agggagacct ttggcgagtg
    17341 ctgatgtccc aagactcatc aagccagtta atatat
//
LOCUS       DAOQOS010000115        17195 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_1665_length_17195_cov_6.409627, whole genome shotgun sequence.
ACCESSION   DAOQOS010000115 DAOQOS010000000
VERSION     DAOQOS010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 17195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17195
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1665_length_17195_cov_6.409627"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            298..3072
                     /locus_tag="PLF66_19965"
     CDS             298..3072
                     /locus_tag="PLF66_19965"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HMZ66377.1"
                     /translation="MSKDILKSVRSLIDYLGSDKNPLYGVWDEMRFDGIYTGNFEDPA
                     YNWGGFGIIPISGFSDGDIAALYPIVGRPIQEYPVVRAPHSGDSTTVIAGSISSFIPN
                     FILNTILDYGGDELDDLEKNKKQILSLATLLGDKQGVTAKILEEIKKEKILSSPSESE
                     RYMICDGKNSFITQYYLLLDDEKKSTDKKAWISLLKKFPFYNPAYYDLFEILSDIEKE
                     KKQALEAAWNYLRGDITFACEYRDIKRAAKFLVEKGGEKNYGKNSEWEAIVAMSEQGA
                     CGGKWYECALKEKEKKNYKTAYTYFKNAAYWIGSDLEEYPATVRKHAFDCVKNLKDKN
                     LFAIFEEIPMKEDKKKMDKILKQYAEIDKKKKESKDISKSMAPLFTAAKNGDLKLVSE
                     YLNKISNINTKEEESGKTVLHYAVKFGHKEIVELLLNKGADLTVRMKTGLNTPLTLAA
                     NYGKLEIAKLLIEKGANVNEENGRNETPFFLACESGNLELAKLLLEKGSNINQRNISK
                     ETPIIIAANKDKDTIVSFLVEKGADLNAEDYSGTTALHWLAVAGKFDLVKLFINKGAN
                     PKIKDSNGETAIDWANKDYPEIAAFLSGKKIKETKWTISESEESILNDMAVKWEMSLK
                     DLMKVDTLNFAYSDFSDSDMANIHLLPKLKTITLLGCKKISDKGLEVITRCKSLEELH
                     LSQTNIKDESLEWIIKCKKLKKLWLDETKITSKSIPKIAIVNNLEWLRLNQINLKGAD
                     IQPLSALKKLKYLELANVSVFDEDLKFLKELKSLEQLDLSYTKITEKTIEQLTGLKNL
                     ENLNLTHTSIGESVFAAFSSIPKIQDIDLTDCSISGEKAGLLSKSKKLLRLNMSGTKV
                     NDQTISQFTSIQSLKVLELENTKLTDSSISELKKMKHLNALILSKTKISSGAKKILFS
                     AIKKDLTLEW"
     gene            3275..4381
                     /locus_tag="PLF66_19970"
     CDS             3275..4381
                     /locus_tag="PLF66_19970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66378.1"
                     /translation="MIKRFVSLFLIFILFSSIEARPTHKHRHKYKKIHKSFAKYSAKK
                     QKRKKLPPVVTANSYNPEIEKKRLMQALKKERDSEKKVELYSQISALYQNSQTYSDQL
                     KAIYYIKKAIDLSKNKWYYYAILANIYSYMKPSEFLNAKKYYEKAIESTDSNYNKSLY
                     YSYISRLYTGREYPNYVEGEKYLLKAIDVTENDYDKANYYIELANLNYNSESKILEYQ
                     NKALEAAPDPDTQSYIYSNIANFYFNNYREFKQEEFDKAVEYMEKAIATTKHAYNKVS
                     YCASLAYGMKFKRDLDTNEIVKIYRRAIEYSINSYDKSYYYGLIANLYTEMQPPENEK
                     AISEYEQAIKHSSDPATIESYKSMVKVLKSRMKK"
     gene            complement(4382..6634)
                     /locus_tag="PLF66_19975"
     CDS             complement(4382..6634)
                     /locus_tag="PLF66_19975"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HMZ66379.1"
                     /translation="MEDKLYAVSEFTDYLEDKENKLTIDQVADHSNLFIQNHINNFSL
                     GFKNHPVWLKVEIANRISKQDFILAAEFAPTDLVTLCEKKEEWFCRTQGDLVPFSQRE
                     IPHRNYLFKIHLDKNEKKTFYIKVKTTSAILFSYTFASEEKYLANSSDSLFLYGIYTG
                     IIILLVIYNFFLYFSFRDLNFLIYIAYIVSYIFFSLGTTGVLNHYLWNDSVILADKVA
                     NISILTGFLSLSIFTYHFLSLKKTKWIQIFFQIIIFILFLGLIAVWINNSNIKYFNML
                     IIPILIPSIYFGFKRYKEGFTPAKYYILAWITLIVIALISILNRLGVISSKYFQADIL
                     QAGAILETLFLSFALSDRIRLLNVEKNKIFELEKATEAAKNASLAKSRFLAVMSHELR
                     TPLNGVSGMANIVLGTNLTEEQREYINIIQSSSNILLNLINDILDYSKIEAGKLKLEA
                     VDFNLQDVIKDVLNIISSVNKNKLRITSIISEDVPKYVKGDMIRLEQVLINLLNNAIK
                     FTEHGSVTLECVNLNNRNEESLIEFRIIDTGIGISEENQKKLFQPFTQADESTTRKFG
                     GTGLGLAISYELVSLMGGQLKLESEISKGSKFYFAIPLKKSDELLLQKSNQNKDIEIF
                     DRENPTEILVAEDSEVNQMINKKIFSSLGYTIHVAENGIKVLEILENQKIDLIFMDVL
                     MPGMDGLTATREIRQNQRHAHIPIIALTANALSEERDRCIEAGMNDFISKPYKSSDIK
                     KLIQKWHPRK"
     gene            7122..7538
                     /locus_tag="PLF66_19980"
     CDS             7122..7538
                     /locus_tag="PLF66_19980"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HMZ66380.1"
                     /translation="MLSKSQARWFFLTGTILSALIFLSLTIDSIQSVYSKPTAELTED
                     VIRGKKIWEKNNCMGCHTLLGEGAYYAPELTKSYERRGPDWIRLFLKDPQAMYPGQRK
                     MVKYNFTDQEISDVIAFLKWNGELDLNGYPAKPNLK"
     gene            7551..8915
                     /locus_tag="PLF66_19985"
     CDS             7551..8915
                     /locus_tag="PLF66_19985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015682184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HMZ66381.1"
                     /translation="MKYKSQKIAYWFFATCMLLLSLQIIYGFIMGFARMGYDGLHDWI
                     PFNAARATHTNLLVVWLLTGFMGAAHYIIPEETGREIYSEKLAYAQLLSLIVVGVIAI
                     IGYHFNYWEGRKFLEIPRPLDYLVVVNVLTFLFNIGMTVWKGEKNSTTSIVLFIGLFS
                     AALLYLPGMIDFNHQTMDSYFRWWVVHLWVEGVWELIMGSILAFLLIKLTGVDREVIE
                     KWLYVIVGLTFLSGILGTGHHYYFIGTPRYWLWVGGIFSALEPLAFLGMALFAINMYK
                     RSGRNHPNKIALFWTIGCAIMSFVGAGFLGFAHTLPQVNLYTHGTLVTAMHGHLAFWG
                     AYAMLVLAIISYAMPLMTGRKLWNNTIGLLGFWISNIGMLGMTGAFAVAGVAQVYLER
                     KMGLDFLAVQKEIEVHFLGLILAACLFTVGIVLYIYNFIQFGTPSDEMVGSNLDSEDL
                     LEKA"
     gene            8916..9695
                     /locus_tag="PLF66_19990"
     CDS             8916..9695
                     /locus_tag="PLF66_19990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015676208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbbQ/NirQ/NorQ/GpvN family protein"
                     /protein_id="HMZ66382.1"
                     /translation="MKTSVPYYRPIEKEVEIFQHLHRNHLPLLLQGPTGCGKSRFVEY
                     MAHSLSLPLITVSCHDETSAVDLLGRYLVKGTDTIWMDGPLTRAVRSGAIVYIDEIAE
                     ARPDTIVALHPLTDHRRSLYLDRKDETIQAPPNFMLVASYNPGYQKGWKEMKPSTRQR
                     FVSISFDYPRQEVEIEIVSEEAKVDKEVAKKLVQLAAKIRNVKDFNLPETVSTRLLVN
                     AGKLIQSGLPTRRACEISIAETLTDETDIIKSIKDLIALIF"
     gene            9699..11444
                     /locus_tag="PLF66_19995"
     CDS             9699..11444
                     /locus_tag="PLF66_19995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66383.1"
                     /translation="MELDQILFKKAYDLFQRIFQQNSSESHSIVYLKDIKERLTSLSS
                     YLSGKKILIKNAEKEGGYSGNIFFLPAQFDHFKEREDNLEYYIFRVIYLSIQKKLNLN
                     WEEEKEKDKLTSRKMAENSFEKIYKEMISEYPAFKERADRIIQLEKDYAKEKDIPLYF
                     IYGRWMYKTLNTVDGNLKRKDFKNQSNEITTEKEAKPIEYKRTIDVNKEEIENYTLNH
                     NFEKVETIEEFKGNWRDLDGSDELNIQEDALKEVDLHDTIRVDDPTHSIYSADFWGMT
                     SASESNLIQNGKYYISYSEWDFKKKKYKENFCRISHSYLTGEKDDSYKACLEKYRNVY
                     TTIKRMFLKTWNDLEKIKHQNTGEEIDLDKIVENYANIHAGKTSSEKVYIQNRKRKKD
                     ISILILMDLSLSTDSYTEGKRILDIEKDSIVLFSELLDEFGVNFQIDGFYSRTRSFCD
                     YITIKSFKDHWKDRRNRIGNIHSIGYTRIGPALRHSTAILKSQNSESKWILLLSDAKP
                     NDYDRYEGKYGIKDIQQSVREANKDNVGIYTLSVTKSDNVLLSEMFGNRYKVLANPNL
                     LPEALTHFYLELLKT"
     gene            complement(11504..12505)
                     /locus_tag="PLF66_20000"
     CDS             complement(11504..12505)
                     /locus_tag="PLF66_20000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66384.1"
                     /translation="MWNNALKIILLFLFPFGLYSEKENTVQVEWESIPGAISYRLEIK
                     DSQEAIVYSEETTGTFLSIKLLVGDYSKRIVSIDKTGEEFPSQWTPLLVLSQKVQMNR
                     IHLEWNNEENADYYILEIQNSNGNTVIRRKVKKTEIEVDLPFGKYKKRILTFDRTGEM
                     VASDWTDLRILKKEDSNYDIVKRSAVFPGWGQVYSEWTYGTEGSRLRGHTFMVLSSIF
                     LGAFFYSAREAENQRNHFNQFATNKVFFSFAAGDSFFSIQDPYNLYVINQLIEKEQIY
                     GRIADRANTSLGLFALVYMIQLTDAFHLQKYELPRFSFQTKTLGPESFYSMSAQFSF"
     gene            complement(12495..13796)
                     /locus_tag="PLF66_20005"
     CDS             complement(12495..13796)
                     /locus_tag="PLF66_20005"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HMZ66385.1"
                     /translation="MNFYDFKFFPEAWIEQDEKKFQNKTNLTTLVLILLLSFYLYWDG
                     QRDPEIGDRKPVGIVTSAINRTERKLGSTVVWKSIIKDKSVYNYDTIRTESDSKTSIE
                     LIDGTKVTLNEDTIIYINTNPEKLDINLIQGSMTVDTMNRKKSEVEKINIVHGDEVIR
                     LEKSLLNVGKKNSNSTLLLELKEGEARLSKSDKNYSLKKGRTVISKKDFIRELEIDSA
                     NTSANKETLFQSFKERQAGFENINGIQIVKSGNQFHVFGQNEKGDFKSTISDRFSTLG
                     KEASKPYALQDPNRILKEALASLGMQVPKNLRSQNRGWELSQKSDLSKKIIITFPANQ
                     SIVDISDMEAFVFSWKNPKPSRPLRIRLFRKDDGVKLFDKLLDSEFYSVEDFTLLKRG
                     YYKFSIYSEEGTLIAESQFQVKLKDIDSGVKFTSPDEIYVE"
     gene            complement(13783..15621)
                     /locus_tag="PLF66_20010"
     CDS             complement(13783..15621)
                     /locus_tag="PLF66_20010"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HMZ66386.1"
                     /translation="MKKLFSRSFIPLNFKLISVNSLIMIGTMSLMILIATEYFKKDSE
                     LRIRENNVMITDAIGARVNAELASILQTVRFMVSDLKEKSLSKSLDQYDDFILSIGIY
                     ESTGYALRPIKMKYNQKILSEAELGESDIDNQIQIYTKHLERALDSESSIQNFSPGLK
                     FPAFAISIPYKKEKKQIVIACIDYRKIRESFETKGSISETMLVNSSGNIIGHPDQNLI
                     IKGENFYDLPVVREMFASNAGSGQIIYSKNEENFIGSYKKVGFAGAGVISIISKDRAL
                     EEVYNIQRRNIILMMIAILIVFFVVYNFAKQITRPVLHLVQVAREIQKGNFQVSFTEK
                     SRDEIGLLEEAFQEMGTGLAEREKVKDALTKFVSKEIAEIALKGEIKLGGEQKVCVIF
                     FSDIRGFTAMSEKMSPEEVVELLNEYLTKMVNCVNLSHGYVDKFIGDAIMATWGVPIS
                     KGNDAENAINGALMMRTALLEMNAVRKSEGKPEIKMGCGLNYGVVVAGQIGSNEKLQY
                     TVIGDSVNLASRVESLNKPFGSDILITQDLYDQVSDTYLTQKMQQIKVKGKSEPQTIY
                     AVLGRKDDPACPQSLEELRELVGIVLPNESAKSSDQVTDEKKYELL"
     gene            16067..>17195
                     /locus_tag="PLF66_20015"
     CDS             16067..>17195
                     /locus_tag="PLF66_20015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012388210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig domain-containing protein"
                     /protein_id="HMZ66387.1"
                     /translation="MKSKIWKFIVILTHLNLGYCLDPSLKQDLNEYIATLLALKKEPP
                     LSILYSNSPYTFTKDLRITTITPITTGLISSCISKPELPIGLVLNPINCSIEGTPTQI
                     QEEKSYTITASNSSGKSAFTTILIAINMGPPSTLVYSGSPYIYTVNLPINPNTPTITE
                     TFTNCVSNPPLPQGLSLNPTTCAISGTPTIIQTATNYTITATNLKGDSTATITITVKF
                     MREWPEIQPILIAQAALGSVATETVGVMGSNYGGVNKWYGAVLAPNGKIYGIPRSATT
                     ILIIDPATDTTSTIPSPDLSGIQKWIGGVLAPNGKIYGIPNAGTSILVIDPNTNTATT
                     FGSFIGGGKWGDVDRRGGAAGGGGAGGGASGIRTGAHVRGAG"
BASE COUNT         5795 a         2822 c         2978 g         5600 t
ORIGIN      
        1 tatattaaaa ttatattctc ttgataaaag taacttagca gcctgatagg ttacgcttcc
       61 tctaatattc taaattcctt tatcatactt atcaatttct atcttttgcg taaggtcagt
      121 taactaatat tattttactg aatcaataat agatatgttt tctaaaaata tactttttag
      181 agcagaatga gtgttaggtg atgcttaaac gaaaaaatag agaaaccaaa ttttctaata
      241 cctttctact tgacttagtt tgcgagtgaa gtaatcttct ggcactgagg aagtccaatg
      301 agcaaagata ttttaaaatc agttcgaagt ttaatcgatt atttagggag tgataaaaat
      361 cctctctatg gagtttggga tgagatgcgt tttgatggaa tctatacggg aaacttcgaa
      421 gatcctgctt ataactgggg aggctttggt atcattccga tctctggttt ttcggatgga
      481 gatattgccg ctctttatcc gatagtaggt agaccgattc aggaatatcc agtcgtgcgt
      541 gctccccatt cgggagattc taccacagtc attgctggat ctatttctag ttttattccc
      601 aattttattt taaatactat tctagactat ggaggagatg aactagatga tttagaaaaa
      661 aataaaaaac aaattctttc acttgcaaca ctactcggag acaaacaagg agtaaccgca
      721 aaaatattag aagaaatcaa aaaagaaaaa attttaagct ctccatctga gtcagaacga
      781 tatatgatct gcgatggtaa gaatagtttt atcactcagt attatttact cttagatgat
      841 gagaaaaaat cgactgataa aaaagcatgg atcagtttac ttaagaaatt tcctttttac
      901 aacccagctt actatgatct atttgaaatt ttatcagata tagaaaaaga aaaaaagcaa
      961 gcgcttgaag ctgcttggaa ttatttgaga ggagacatta catttgcctg tgaatataga
     1021 gatattaaaa gagccgcaaa gtttttagta gagaagggag gagaaaaaaa ttatgggaag
     1081 aattcagaat gggaagcaat agtcgctatg tcagaacaag gagcctgtgg cggcaaatgg
     1141 tatgaatgtg ctctgaaaga aaaagagaaa aaaaattaca aaaccgctta tacttatttt
     1201 aaaaatgcag cgtattggat tggatctgat ttggaggaat atcctgctac tgttcgaaaa
     1261 catgcttttg actgcgtaaa aaatctaaaa gataaaaatc tatttgctat cttcgaagaa
     1321 attccaatga aagaagataa gaaaaaaatg gataaaatct taaagcaata tgcagagatt
     1381 gataaaaaga aaaaagaaag taaagatatt agtaaatcaa tggctcctct ctttaccgcc
     1441 gctaagaatg gagatttgaa attagtttct gaatatttaa ataaaatttc gaatatcaat
     1501 acaaaagaag aagagagcgg caaaactgtg ttacactacg ctgtcaagtt tggacataaa
     1561 gagattgtag aattactttt aaacaaaggt gcagatctaa cggttcgtat gaagactgga
     1621 ttaaatactc ctcttacact cgcggcaaat tatggtaagt tagagattgc taaactttta
     1681 attgagaaag gagctaatgt aaacgaggaa aatggacgaa atgaaactcc tttttttcta
     1741 gcctgcgaga gtggaaattt agaattagca aaactacttt tagaaaaagg atcgaatata
     1801 aaccaaagaa atatctcaaa agaaacaccg attatcattg ctgcaaacaa agataaggat
     1861 actatcgttt cctttctggt agaaaaagga gcagacttaa acgcagaaga ctatagtggc
     1921 actacagccc tgcattggct tgcagtagca ggaaagtttg atcttgttaa actttttata
     1981 aacaaaggag ccaatccaaa gattaaagat agcaatggag aaacagccat tgattgggct
     2041 aacaaggatt atccggaaat tgcggctttt ctatctggaa aaaaaataaa agaaacgaag
     2101 tggactattt ctgagtcaga agaatctatt ttaaatgata tggctgtcaa atgggaaatg
     2161 tctttgaagg atttaatgaa agtagataca ttaaattttg cctactcaga cttttccgat
     2221 tcagatatgg caaacatcca tttgctaccc aaattaaaaa cgatcacact tctaggttgc
     2281 aaaaagatat cagataaagg tctagaagtt ataactcgtt gtaaaagttt agaagagctt
     2341 catctttccc aaacaaatat aaaagatgaa tctttggaat ggattataaa atgtaaaaaa
     2401 ttaaagaaat tatggctgga tgaaaccaag atcacatcta aatcgattcc taaaattgca
     2461 attgtaaaca atttagaatg gttgaggtta aaccagatca atttaaaagg tgcagatata
     2521 cagccattat ccgcacttaa aaaattaaag tatttagagt tagcaaatgt ttctgttttt
     2581 gatgaggatc taaaatttct aaaagaactg aagagtctgg aacaacttga tctgagctat
     2641 acaaaaatta cagaaaagac aatagaacaa ctgactggat tgaaaaactt agaaaaccta
     2701 aatctcactc atacatcaat aggtgaatct gtgtttgccg cattttctag cattcctaaa
     2761 atacaagaca tcgatttaac tgattgctct atttctgggg aaaaggctgg gcttttatct
     2821 aaatccaaaa aattacttcg actgaatatg agtggaacga aagtaaatga tcagactatc
     2881 tctcaattca catctattca atctcttaag gttttagaat tggaaaatac taaactgaca
     2941 gactcttcga tttcagaatt aaagaaaatg aagcatttga atgcactgat cctatcaaaa
     3001 acaaagatat caagtggagc aaaaaagatt ttattttctg caattaagaa agatttaaca
     3061 ttggaatggt agtattcttt ttttaaaaca ggatgctttt ttcctaccaa aagaaattag
     3121 ttcttgaaaa aaaataaggc atccataatc tacttaactc acttcaaatt cgttctaagc
     3181 cttcctgtgt ttattctaag cagggagact tttgatttaa tctaaatgtt ttaatactcg
     3241 aagtggtgtg tattttttaa aaaaagagga ttttatgata aaaaggtttg ttagtttatt
     3301 tttaattttt attcttttta gctcaataga agcaagacca acgcataaac ataggcataa
     3361 atataagaaa attcataaat cctttgctaa atattctgca aaaaaacaaa agagaaaaaa
     3421 attacccccc gttgttacgg ctaatagcta taatccagaa atagagaaaa aaagattaat
     3481 gcaagctctt aagaaggaaa gagacagcga aaagaaagta gagttataca gtcaaatatc
     3541 tgcactttac caaaattctc aaacgtattc tgatcagcta aaggcaattt attatattaa
     3601 aaaagcgatc gatctatcaa agaacaagtg gtattattat gcaattcttg cgaatattta
     3661 cagttatatg aaaccaagtg agttcttaaa tgcaaagaag tattatgaga aagcaattga
     3721 atctacagat agtaattata ataaatctct ttattattct tatatatcca gattatacac
     3781 tggaagggaa tatccaaatt atgtagaagg agagaagtat ttattaaaag cgatagacgt
     3841 aacagagaat gattatgaca aagcaaatta ttatattgag ctagcaaatc taaattacaa
     3901 ttcagaatct aaaatcctcg aatatcaaaa caaagcctta gaagcagcac ctgatccaga
     3961 tacgcaatcc tatatatact ctaatattgc taatttttat tttaacaatt atagagaatt
     4021 caaacaagaa gagtttgata aagctgtaga atacatggaa aaagccattg caactacgaa
     4081 acatgcttat aacaaagtaa gttattgtgc aagtctagct tatggaatga agttcaaacg
     4141 tgatttagat acaaatgaaa ttgtaaaaat atacagaaga gcaattgaat atagtataaa
     4201 ctcttatgat aaatcatatt attatggatt aatagccaat ctatatacag aaatgcaacc
     4261 gcccgaaaat gaaaaagcaa tttcagaata tgagcaagcg attaaacatt catctgatcc
     4321 tgcaacaatt gagtcttata agtcgatggt taaagtttta aaatctagaa tgaagaaata
     4381 attattttct tggatgccat ttttgtatta attttttaat atcactgctc ttgtatggtt
     4441 tggaaataaa gtcattcatt cctgcttcta tacatcgatc tctttcctcg gaaagagcgt
     4501 tagctgttaa agctattatt ggaatatgtg catgtctttg attctggcga atttcccttg
     4561 ttgccgtgag tccatccata ccaggcatta atacatccat aaagattagg tcgatctttt
     4621 gattttccag aatttccaag actttgattc cattttctgc aacatgaatt gtataaccta
     4681 agctagagaa aatttttttg ttaatcattt ggtttacttc tgaatcctct gccactagaa
     4741 tttcggttgg attttcccta tcaaaaattt caatatcttt gttttgattc gatttttgca
     4801 aaagaagttc atctgatttt ttgagtggaa ttgcgaaata aaatttactt cctttcgaga
     4861 tttcactctc caattttaat tgtccgccca tgagtgaaac tagttcgtag gagattgcaa
     4921 gaccaagtcc tgttcctcca aactttcgag tggttgattc atctgcttgt gtaaaaggct
     4981 gaaatagttt tttttgattt tcttcagaaa ttccaattcc tgtatcaata attctaaatt
     5041 caatcagaga ttcctcattt ctattattta aatttacaca ttctagggtt acagatccat
     5101 gctccgtaaa tttaattgca ttgtttagta aattaattag aacttgttct aaacgaatca
     5161 tatctccttt tacatattta ggcacatcct cggagataat acttgttatc cgcaatttat
     5221 ttttatttac gctgcttata atattcagca catctttaat aacatcttga agattaaaat
     5281 caacagcttc cagttttaat tttcctgctt ctattttaga ataatctaat atatcattga
     5341 ttagatttag taaaatatta gagctagatt gaatgatatt tatatattca cgctgttcct
     5401 ctgttaaatt tgttcctaaa acaatatttg ccatgccact tactccattt aatggagttc
     5461 tcaattcatg actcataacg gctagaaatc tagattttgc gagtgaggca ttttttgcgg
     5521 cttccgttgc tttttctaat tcaaaaattt tatttttttc tacgttaagc aatcgaattc
     5581 tatcggataa tgcaaatgat aaaaaaagag tttctaatat cgctccagct tgtaatatat
     5641 ctgcctgaaa atactttgat gaaataactc caagtcgatt taaaatacta atcaacgcga
     5701 ttactattaa tgtaatccat gctagaatat aatatttcgc tggagtaaaa ccttccttat
     5761 atcttttaaa tccaaaataa atagaaggga ttagaattgg tatgattaac atattaaaat
     5821 attttatatt agaattgttg atccaaacag caatgagtcc taagaataga atgaaaatga
     5881 ttatttgaaa aaagatttga atccattttg tttttttcaa acttaaaaaa tggtaagtaa
     5941 aaatactgag agaaagaaat cctgtaagaa ttgaaatatt tgctacttta tccgctaaaa
     6001 ttacagaatc attccataga taatgattta aaacacctgt tgttcctaaa ctaaaaaata
     6061 tataagaaac aatatatgca atgtatatta gaaaatttaa atctctaaaa ctgaaatata
     6121 aaaaaaagtt ataaatgact aataaaataa taatgccagt gtagatacca tatagaaaaa
     6181 gggaatcaga agaattagct aaatactttt cctcagacgc aaaagtatag ctaaataaaa
     6241 tggcacttgt agttttaact tttatataga atgttttttt ctcgttttta tctaaatgta
     6301 ttttaaataa ataattacga tgtggtattt ccctttgtga aaaaggaact aaatctcctt
     6361 gtgtcctgca aaaccattcc tctttttttt cacagagtgt aactaaatct gttggtgcaa
     6421 attctgctgc gagtatgaaa tcctgttttg aaatccgatt ggctatttcc acttttaacc
     6481 agacaggatg atttttaaat ccgagagaaa aattgtttat atgattttga ataaataaat
     6541 tggaatgatc tgcaacttgg tctatggtta atttattttc tttatcttct aaatagtctg
     6601 taaattcact tacagcatat aacttatctt ccattatctc gagtgttttg gcacttatag
     6661 taattgagag taaaaaaaag aatccaataa actgtaaaaa tttcaaatac atagatcacc
     6721 attataattc ttatatttct caatcagtgc aattgaaatt taaagttata attgaaaaat
     6781 taaagaaatt tttgaaaaat ttccaagagt tttccctaca tataatatgg atattatttg
     6841 gagtagatag tttgaaagcg ttaagaacat tgctttagaa ttcgcacttt tttgtggaat
     6901 tcaaatttaa gaatttcatt tcttgaatga taatcttagt attcgctctt taaatttgat
     6961 aaatctctcg attgtcagta aatcaatcta atttcgtttt ttattttgat atagattgat
     7021 ctaggtcaat tacacctgaa gcaaaaaggt tagaatctat tttattgaaa agggattaga
     7081 tattttctgc tttcaataaa ataataaaat agggtgctaa tatgcttagt aaatcgcagg
     7141 cgagatggtt ttttctcacc ggcacaattc tcagtgcttt gattttttta tcactaacga
     7201 tagactccat tcaatctgtt tattctaaac ctacagctga acttacagaa gatgtgattc
     7261 gtgggaaaaa aatttgggag aaaaataatt gtatgggatg ccatacactc ttaggagaag
     7321 gagcttatta tgctcctgaa ttgactaaat cctatgagag aagaggtcct gattggattc
     7381 gtttattttt aaaagatccc caagcgatgt atccagggca aagaaaaatg gtgaaatata
     7441 attttaccga ccaagaaatt tcagatgtaa tcgctttctt aaaatggaat ggagaactcg
     7501 atctgaatgg atatcctgcc aagccaaatc ttaaataaaa ggagaaagaa atgaaatata
     7561 aatcacaaaa gattgcttat tggttttttg caacctgtat gcttctgctt agtttgcaaa
     7621 taatctatgg attcatcatg ggatttgctc gcatgggtta cgatgggtta cacgattgga
     7681 ttccgtttaa cgctgcaagg gcaactcata cgaacttgtt agtcgtttgg cttttaactg
     7741 gctttatggg tgctgcacat tatatcattc cagaagaaac aggaagagaa atctattctg
     7801 aaaagttagc ttatgcacaa cttctatcac ttattgttgt aggagtgata gcaattattg
     7861 gatatcattt taattattgg gaaggtagaa agtttttaga aatacctaga ccacttgatt
     7921 atctggtcgt agtgaatgtt cttacctttt tatttaatat aggtatgacg gtatggaaag
     7981 gtgaaaaaaa ttctacaacc tccattgtat tatttatagg tctattctct gctgctcttc
     8041 tctatttacc gggtatgata gatttcaatc atcaaacaat ggattcttat ttcagatggt
     8101 gggtcgttca tctttgggta gaaggtgtat gggaattaat catgggttca attttagcat
     8161 tcctattaat taaattgact ggtgtagata gagaagtgat tgagaaatgg ttgtatgtaa
     8221 tcgtaggact aacgtttctc tctggtattt taggaacagg tcaccactat tattttattg
     8281 gaactcctag atattggctt tgggttggtg ggattttctc tgcattagag cctttagcat
     8341 ttttaggtat ggcactcttt gcaatcaata tgtataagcg tagtggaaga aatcatccca
     8401 ataaaattgc tcttttctgg acgattggtt gtgcaatcat gtcctttgta ggagctggat
     8461 ttttaggttt tgctcataca ctaccacaag taaatctcta tactcatgga acgttagtaa
     8521 ctgcaatgca tggtcatttg gctttctggg gagcttacgc gatgttagtt cttgcgatta
     8581 tttcttatgc gatgcctctt atgacaggta gaaaactttg gaataataca attggactct
     8641 taggattttg gatttcaaat attggaatgc ttggaatgac aggagccttt gccgttgcgg
     8701 gtgtcgcaca agtatatctc gaaagaaaga tgggactcga cttcttagcc gttcaaaaag
     8761 aaattgaagt tcacttcttg ggactcatac ttgctgcctg tctttttaca gtaggaattg
     8821 ttctatacat ttacaatttc attcaatttg gaactccttc tgatgagatg gttggatcaa
     8881 atcttgattc agaagatttg ctagagaagg cataaatgaa aacttcggtc ccatactata
     8941 gaccgattga gaaagaggta gagatttttc agcatttgca tcgaaatcat ttacctcttt
     9001 tattacaagg tccgacaggc tgcggcaaat ctcgatttgt cgaatacatg gcacatagtc
     9061 tatctttacc attaattacg gtctcttgtc atgatgagac ttcagcagta gacttgcttg
     9121 gtagatattt agtaaaagga acagatacga tctggatgga tggacctctg actcgagcag
     9181 tccgttctgg tgctattgtt tatatagatg aaattgcaga agcgcgtcca gatacgatag
     9241 ttgctttgca tccgctaact gatcatagaa gaagtttata tctagataga aaagatgaaa
     9301 cgatccaagc tcctcccaac tttatgttag tagcttctta taaccctgga taccaaaaag
     9361 gttggaagga aatgaaacca tctactcgtc aaagatttgt gagcatcagt tttgattatc
     9421 caagacaaga agtagaaatc gaaatcgttt cagaagaagc aaaagtagac aaagaagttg
     9481 caaaaaaatt agtccaactt gcagcaaaga ttagaaatgt aaaagatttt aatctaccgg
     9541 agacagtttc tactcgtctt cttgtgaatg caggtaagct gattcaaagt ggtcttccta
     9601 cccgtcgtgc ctgtgaaata tcgatagccg aaacactcac agatgaaact gatataatta
     9661 aaagcattaa ggatttaatt gctttgattt tttagagtat ggaactagat cagattcttt
     9721 ttaaaaaagc ttatgatttg tttcaaagaa tctttcagca aaattcctct gaatctcatt
     9781 caatagttta tttgaaagat attaaagaga ggctaacaag tcttagctct tatctttctg
     9841 gtaaaaaaat attaataaaa aatgcagaga aagaaggagg ctattctgga aatatatttt
     9901 ttcttcctgc tcaatttgat catttcaaag aaagagaaga taatttggaa tattacatct
     9961 ttcgagtcat ctatttgtct attcagaaga aattaaatct aaattgggaa gaggaaaaag
    10021 aaaaagacaa acttacatct agaaaaatgg ctgagaattc ttttgaaaaa atctacaaag
    10081 aaatgatttc agaataccca gcttttaaag aaagagcaga tcgtatcatt caattggaaa
    10141 aagattacgc aaaggaaaaa gacattcctc tctattttat ttatggaaga tggatgtata
    10201 aaacgttaaa tacagtagat ggaaatttga aacggaaaga ttttaaaaac caatcaaatg
    10261 agattacaac agaaaaagaa gcaaagccaa ttgaatacaa aagaacgata gatgtcaaca
    10321 aagaggagat tgaaaattat actttaaatc ataattttga aaaagtagaa accattgaag
    10381 agttcaaagg caactggcgt gatttggatg gatcggatga attaaacatt caagaagatg
    10441 ctttaaaaga agtagatttg catgatacaa ttcgagtaga tgatccaact cattctattt
    10501 attccgccga cttctgggga atgacgtctg catctgaaag taatttgatt caaaatggaa
    10561 agtattatat ctcttattca gaatgggatt tcaaaaaaaa gaaatacaaa gaaaattttt
    10621 gtagaatttc tcattcttat ctaacaggag aaaaagatga ttcctataaa gcctgtctag
    10681 agaaatatag aaatgtttat actacaatca aaagaatgtt tttaaaaaca tggaatgatt
    10741 tagaaaaaat aaaacatcaa aacacaggtg aggaaatcga tctagacaag attgtagaaa
    10801 attatgcaaa tatacacgca ggcaaaactt cttctgaaaa agtatatata caaaatagaa
    10861 aacgaaaaaa agatatatca attttaattt taatggattt gagtttatcg actgattctt
    10921 ataccgaggg aaaaagaatt ttagatatcg aaaaagattc aattgtatta ttttctgagt
    10981 tactcgatga gtttggagtc aattttcaaa tagatggatt ctattctaga actagaagtt
    11041 tttgtgatta tattacaatc aaaagtttta aagatcattg gaaagatagg cgaaatagaa
    11101 tagggaatat tcattctatt ggttacacga gaattggtcc tgcccttcgt cattctacag
    11161 ccattctaaa aagtcaaaat tctgaaagca aatggattct acttctatcc gatgcaaagc
    11221 caaatgatta cgaccggtat gaaggtaaat atggaataaa agatattcaa caatctgttc
    11281 gagaagcgaa taaagacaat gtgggtatat acacattatc cgttacaaag tcagataacg
    11341 ttctattgtc tgaaatgttt ggaaatcgct acaaagtatt ggcaaatcca aatctattac
    11401 cagaagcact gactcatttc tatctggagc ttttaaagac atgaagcctt gctttgtctt
    11461 tctagctcta tcgctaattc catcaactta atccaaaatt ctctcaaaaa gaaaattgag
    11521 cagacataga ataaaatgat tctggtccaa gagtcttagt ttgaaaagaa aaccttggaa
    11581 gctcatattt ttgaagatga aatgcatctg tgagttgaat catatacacg agagcaaata
    11641 gtccgagaga ggtatttgca cgatctgcta ttctaccata gatttgttct ttttctatga
    11701 gttgattgat tacatagaga ttgtatggat cctgaatgga aaagaaagaa tcaccggcag
    11761 caaaactaaa aaatactttg tttgttgcaa attgattaaa atgatttctt tggttttctg
    11821 cttcccttgc agaataaaaa aaagctccta gaaaaatcga actaagaacc atgaaggtat
    11881 gtcctcgcaa tctacttcct tctgttccgt aggtccattc agaatacact tgtccccatc
    11941 ctggaaaaac cgcagaacgc tttacaatat cataattact atcttctttt tttaggattc
    12001 ttaaatccgt ccaatcggag gcaaccattt cacccgttcg atcaaaggtt aagattcttt
    12061 ttttgtattt tccgaaagga agatctactt cgatttctgt ctttttgact tttctacgaa
    12121 tgacagtatt tccatttgaa ttctgaattt ctagaatata gtaatccgca ttttcttcat
    12181 tgttccattc taaatgaatt cgattcatct gcactttttg tgagagcact aagagaggag
    12241 tccactgaga cggaaattct tcccccgttt tatcaatcga aacaattctt ttagaataat
    12301 cgccaacaag tagcttgata ctcaaaaaag tcccagttgt ttcttccgaa tacacaatcg
    12361 cttcttgact atccttgatt tccaatcgat aggaaatcgc acctggtata ctttcccatt
    12421 caacctgcac agtgttttct ttttctgaat acagaccaaa agggaaaagg aataaaagaa
    12481 taatctttag agcattattc cacataaatt tcatcgggtg aagtaaactt gactcctgaa
    12541 tcaatgtctt tcaatttcac ttgaaattga ctttctgcga ttaacgttcc ttcttctgaa
    12601 tagatagaaa acttataata cccccgtttc aaaagggtaa aatcctctac agaataaaat
    12661 tcagaatcga gaagtttatc aaatagcttc actccatcgt ccttgcgaaa aagtcgaatg
    12721 cgaagaggcc tagatggttt tggattcttc caggaaaaaa caaaagcttc catatcacta
    12781 atatctacga ttgattgatt ggcaggaaaa gtaatgatga tttttttgga caggtctgat
    12841 ttttgcgata gttcccatcc tctgttttga gatcgtaaat tttttgggac ttgcattcct
    12901 agactagcca aagcttcttt tagaatgcga ttcggatctt gcaaagcgta gggtttactt
    12961 gcttcctttc caagtgtgga aaaccgatct gagattgtac ttttaaaatc tcctttttca
    13021 ttctgcccga atacatgaaa ctgattccct gattttacga tctgaattcc atttatattt
    13081 tcgaatcccg cctgacgttc tttaaaggat tgaaaaagag tttctttgtt ggcactagta
    13141 ttagcagaat caatttcaag ctctcgaatg aaatcttttt tagaaataac tgtgcgacct
    13201 ttttttaagg aataattttt atctgattta ctcaggcgag cttctccttc tttcagttct
    13261 agcaaaagag tagaattgga atttttcttt cctacgttga gaagactttt ctctagacga
    13321 atgacctcat ctccatgaac aatatttatt ttttcgacct cggatttttt tcgattcatt
    13381 gtatccacag tcatcgatcc ttgaatcaaa ttgatatcca atttttcagg attggtattg
    13441 atatatataa ttgtgtcttc atttaaagtt actttcgtgc catcaatgag ttcaatcgat
    13501 gtctttgaat cactctcggt tcgaatcgta tcataattat aaactgattt atctttgatg
    13561 atagatttcc aaacaacagt ggagcccaat tttctttcgg tgcgattgat ggctgaagtt
    13621 acaattccca ctggtttgcg atcaccgatt tctggatccc gttgaccatc ccaataaaga
    13681 taaaaagaca aaagcaagat cagaacaaga gtagttagat ttgttttatt ctggaacttt
    13741 ttttcatcct gctcgatcca agcttctgga aagaacttaa aatcatagaa gttcatattt
    13801 tttttcatct gttacttgat cagaagattt ggctgattca tttggcaata caatgcctac
    13861 tagctctctg agttcctcga ggctttgagg acaggctgga tcatcctttc gtcctaaaac
    13921 ggcataaatg gtttgaggtt cactttttcc tttcacttta atctgttgca ttttttgggt
    13981 taggtaggtg tcgctcactt gatcataaag atcctgagtg atgagtatat ctgatccgaa
    14041 cggtttattt agcgattcca ctctcgatgc tagattcacg ctatctccga ttacggtata
    14101 ttgaagtttt tcgtttgatc caatttgacc cgcgaccacg actccgtaat tgagtccaca
    14161 gcccattttt atctctggct tgccttctga ttttcgaact gcattcattt ctagtagtgc
    14221 tgtcctcatc ataagcgcgc cattgattgc attttctgca tcgtttcctt ttgaaatagg
    14281 cacaccccaa gtcgccataa tcgcatcccc gataaactta tccacgtaac catgcgaaag
    14341 gtttacacag ttaaccattt ttgtcagata ctcgtttaac agttcgacga cttcttctgg
    14401 agacattttc tcagacatgg cagtaaatcc ccgaatatca gaaaagaata tcacacaaac
    14461 tttctgttcg ccccccaatt tgatttcccc tttcagtgca atttctgcaa tttctttgga
    14521 gacaaattta gtgagagcat ccttcacttt ttccctttcc gcaagccccg ttcccatttc
    14581 ctgaaaggct tcttcgagaa gtccaatctc gtctctagat ttttcagtaa aagaaacctg
    14641 aaaatttcct ttttgaattt cccgagccac ttgaacaaga tgaaggacag gtcttgtaat
    14701 ctgttttgca aaattgtaaa cgacaaagaa tacgatgagg atagcaatca tcatgagtat
    14761 aatatttctt ctttgaatat tataaacttc ttctaaggct ctgtcttttg aaataataga
    14821 aatcactcca gcaccggcaa acccgacttt tttataagag cctatgaaat tttcctcgtt
    14881 tttagaatag atgatttgtc cacttcctgc attcgaagca aacatttctc tcaccactgg
    14941 tagatcataa aaattctcgc ctttaatgat tagattctga tcgggatgac cgatgatatt
    15001 tcctgaggaa ttgacaagca tggtttcgga aatacttccc ttcgtttcaa aagattctct
    15061 gatttttcta taatcaatac acgcgattac gatttgtttt ttttcttttt tatagggaat
    15121 ggaaatagca aatgcaggaa attttaaacc aggactaaaa ttctggatgc tagattcaga
    15181 atctagggct ctttctagat gtttggtata aatctgaatt tggttatcaa tgtccgattc
    15241 tcccaattct gcttcggaaa gaattttctg attgtatttc atcttgattg gacgcagtgc
    15301 ataacctgtt gactcataga ttccaatgga aagaataaaa tcatcatatt gatctaaaga
    15361 tttggacaaa ctcttttctt ttaaatcaga taccataaaa cgaactgttt gcaaaatcga
    15421 ggcaagctca gcgtttactc ttgcgcctat cgcatcagtg atcattacat tgttttcacg
    15481 gatacgaagt tcactatcct ttttgaaata ctctgttgca atgagaatca taagactcat
    15541 cgtcccaatc atgataaggg aattgacaga gatcagtttg aaattgagag gtataaaact
    15601 tctactaaat aactttttca ttgttagtaa attagcaatc ccaagtttat tgaattttgg
    15661 cgtttcattc tttgggaatt tttaattttt aacgaacctt acattttata tctttttaaa
    15721 ctgtcgatag tattcgaatc tgtcaaatca atttaaaatc agttctactt aggatgagcc
    15781 attttagaaa acagtaacat tatctttttt actattcctt gttattccac acaagaaaat
    15841 gtgtctgttt tttgagtaaa tacataccaa tgctagtgag tcgttgcgcg ttgcgcatga
    15901 cttttgtaga aaagcgaatt ggtatagacc atttcatttt tttactttag caatgcgcaa
    15961 tgctttttga gaaatggtat attaacgaaa taatcatcgt ctaagttttg tgcttgatag
    16021 gttaaatgaa aaaaatacaa actcgaatag gttagtcggt ggtaagatga aaagcaaaat
    16081 ttggaaattt atcgtaatct tgactcatct gaatttagga tactgtcttg atcccagctt
    16141 gaagcaagat ttaaatgaat acatcgcaac attgcttgct ctaaaaaaag aacctcccct
    16201 ttctatttta tattccaatt caccctatac ctttacaaaa gatttgagga ttacaactat
    16261 tactccgatt actactggtc tgatttccag ttgtatttct aaaccggaac taccgatcgg
    16321 attggttctg aatcctatca attgttccat tgagggaact cccacacaaa tacaggaaga
    16381 aaaatcgtat acaattactg cgagtaattc cagtgggaaa tctgctttta ctaccatcct
    16441 gattgcaatc aatatgggac caccatctac cttagtctat tctgggagcc cttatatcta
    16501 caccgtaaat cttccgatta atccaaacac tcctaccatt acggaaactt tcacaaactg
    16561 tgtgtctaac ccacctctgc ctcagggttt gagtttgaat ccaactactt gtgccattag
    16621 tggaactcct accataattc aaaccgccac aaattatacg attacagcca caaatttgaa
    16681 gggagattca acagctacca tcacaataac ggttaaattt atgagagaat ggccggaaat
    16741 tcagccaatc ttaatcgcac aagccgcttt gggctctgtg gcaacggaaa cggttggtgt
    16801 gatgggaagt aattatggag gtgtaaacaa atggtatggg gcagttcttg ctcccaatgg
    16861 aaagatctac gggattccaa gatcagcaac tactatccta atcatcgatc ccgctactga
    16921 tacaactagc acgattcctt cccctgatct atcaggcatt cagaagtgga ttggaggagt
    16981 acttgccccg aacggaaaaa tctatggaat tccaaatgca ggaacgagca tattagtaat
    17041 tgatccaaat acaaatacag caacgacctt tggtagtttt attggaggag gcaaatgggg
    17101 ggacgtggat cgccggggcg gtgctgctgg tggtgggggt gctggcggcg gcgcgagcgg
    17161 gattcgaact ggcgcgcatg ttcgaggcgc tgggc
//
LOCUS       DAOQOS010000116        16704 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_1728_length_16704_cov_5.396120, whole genome shotgun sequence.
ACCESSION   DAOQOS010000116 DAOQOS010000000
VERSION     DAOQOS010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 16704)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16704)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16704
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1728_length_16704_cov_5.396120"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /locus_tag="PLF66_20020"
     CDS             <1..207
                     /locus_tag="PLF66_20020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66388.1"
                     /translation="VISVLWSLSLFQSIFFAIKEFGANPIFSINMVKEKNVEEARESL
                     GLLIVFLVMIWKYYEAKKHSKEQK"
     gene            510..1952
                     /locus_tag="PLF66_20025"
     CDS             510..1952
                     /locus_tag="PLF66_20025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002746633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HMZ66389.1"
                     /translation="MKKLLLSFLVLIFLVLSYTILFSEKGISIKKQADNDNLPNFDQI
                     AEEAALDLQKYIQIKTIRGNELESALFLKIILEKYNIPSKIITPPNEPSRANLFAELP
                     GTEEEGGVIFMNHMDVVEAEAVEWKYPPFNGIRIKDRIYGRGAVDMKGLGIMQLYAML
                     LIKKSGIKLKHKLMFAALADEESRSAKGAQFIVKEHKELFKGYEYIHNEGGTGTDSVA
                     VPGSKIFNLQYAEKGILWLELEAKAESGHGSTPPIAYAAKNMVNFLTELQEFSKETII
                     SDITAAFFYQMGEASPFPNSFVLKRSRNPIIQLALNKVINSNRHLRAMTSNTVSITGI
                     DSDPIGINVITNHTKGSVDIRLLPGVDPNDYLNKIRKIAEKYKIAISVKHSEAASVSP
                     MDSKFFQILAGTAVKVIPNAIVTPFLSPGTTDSSYFRLNGFKCYGLIPALLTDVELDG
                     IHGKDESMRVGHLKTGIQILYETIINYNKL"
     gene            1949..4975
                     /locus_tag="PLF66_20030"
     CDS             1949..4975
                     /locus_tag="PLF66_20030"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HMZ66390.1"
                     /translation="MNSILFSYYAPGVFFDTLIALLISIFLIFKKEKSIATYWLIGFF
                     LGEFSLFAAYTASYSIFNEVGAYHRYFSCLVMFGNASLVGFSYFYQKNDKPKEGMIVI
                     SLSFIIALVAYIHFILKTISMEKIYNPEAHIYTFDFGKEVGIVIALLFLVSLINLIRK
                     TIRYSTHSDFFGKWTIKPQRLTGYPVYYLKSFFLGIYKIYSPRGKEAESTRDFAIPIF
                     LQILIALLNVLVKSGKISYELYALLFSFSTMIVIYNIAIVYLNHSPEPTSFMVKLVGI
                     SLGAILVVLAIVGQISLNDREENYNTIHLLKINQAKIALNKGNWEDIPSDVEYILMKP
                     KNSSFFSKDTTILLLRDPAVTLEDIYKEVDKEKEKTIQAKISANKDKTLSTEQKYQIA
                     LEEINSYYPPEQKRLYRQINHKLFTQFDFVEEENRYEIGFNLRVYREYIHSTSKNIII
                     TMISVFFSVLAFFPMLFKTSLVSPLRKLLDGVKNVNDGDLSVQVPVKSYDEIGFLAVS
                     FNSMVKSIRQAREDLQDYAENLELRVDERTREVREKMELIESLKIQQDADYFLTSLLA
                     KPLFYNANKSTLVKTEFIIKQKKQFEFKNKRAELGGDICITGNLRLGTKENYKRYTMA
                     MNGDAMGKSMQGAGGSLVMGVMMNSIMARSASQNKILNQTPEEWLRLVYQELHSVFKS
                     FNGSMVISAVIFIIEDETGKGYFINAEHPFSVLYRDGRASFIEKELQLRKIGFDIDTE
                     FKYETIQLQERDVIILGSDGRDDIDLTPKESVRTINEDETIFLQLVEKAESDISKIVD
                     LIGEVGTIIDDLSLLRIGYKEEIVEIEKVKEPEVFSESDITIQVDKDGFTKTRTDIHT
                     NIALNTDNAYLEGKALSEKGRIDEAINILYSAFTLDKTHSELTRLLGVLAFKAKDYKK
                     AIEALKTYTSHFEREEELLYSLAISCKKMEQYKDSLSVSTLLERINPKLIRNLLNLAD
                     ANRLIGNFQKAKEFSERVISMDPKNEIALKILKIVS"
     gene            5125..5520
                     /locus_tag="PLF66_20035"
     CDS             5125..5520
                     /locus_tag="PLF66_20035"
                     /inference="COORDINATES: protein motif:HMM:NF024183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HMZ66391.1"
                     /translation="MSDTIISSVFICPKCKYTNPSKSKFCMQCGSPLSDATKQTKKPK
                     LILLNDSKVEKEFTIESKSIIGKGGDISVDGANFKDNLAELKLDGDRLVLTAMSNDVF
                     MQIAANKPLELANGTILKIGDKVFKVDLQ"
     gene            5517..9011
                     /locus_tag="PLF66_20040"
     CDS             5517..9011
                     /locus_tag="PLF66_20040"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase fold protein"
                     /protein_id="HMZ66392.1"
                     /translation="MNVFCPNCGTENPSIASFCANCGFNIKSLTKQSDIEILREALSP
                     KEYKVERKISGGSQSDPYLCEYLSTGEKYVIRILKPNQSDEVRNLFVTGLQLNAQLDH
                     PNILKIKEIGEINGLPYFISNYADNGSLSWVLKTYGTNGMPIEEAIKYIIKVLKGLDY
                     IHTRNVIHQNIKPDAVFITKGGEPVIGEFVMAKKVEEGQKVKTESLVGTLEYMSPERC
                     KLARSIDRRSDIYSVGIMLYQLLTGELPFTGDTQTLIYKQTNEKLPNITYKLLAKGLN
                     SIIAGKEKLYLAAGEIQSILEKACAKGKSSRFNTALEFATVLEKFLEFVSIPTVTNKK
                     ENRWKALNNYRADHNANNSDDRLGITNAVDAFARLISSKSVHPPLSIGLFGNWGAGKS
                     FFMNKLTKEIDRLTESMRSAAKNGVPAKDLTFYENIVQIEFNTWHFVDANLWASLVNH
                     IFSNLKIKGEPLDEVRERRNFLIKRIESEKNGLLDLEKDEEKFSNQIEDIKLEIESES
                     KNYKTIVASLFDKHFDEAVKEKFKPDEIKLWAKRAGVEYNPDEPIGAQMHRIYQETDN
                     LKNLPIFEKIKIQFKGYFYFAVIAFILFLIGLICSIGFYIYSETFIGVWIQSFAFNTK
                     EWILGVIGSITAMLSPLLPIYYGLKSWSQKYLNWGPNFLKKIKDTIPDLNKELENKEK
                     EKQEKFLELENKKKSFETKKENARLEIQKLESELEKTSDGDYLSTFIRNRMGTDEYTK
                     HLGLQAKIRKDFEQLSSLIDKYNKSILAGEVKDNDGYMINRIVLYIDDLDRCPPDKVV
                     EVLQAVHLLLSFPAFVVVVAVDARWVSQSLRIGYRDLFGEEISLDTDGDGIPDSFRAT
                     PHDYLEKIFQIPFWLYPMNIEGRRNLLAQLMESNLEATNDLPQEPVLEDKTNLIENKT
                     NLTNNEKEIEQREDPGFKTDILKEDESILEENEYTFKLEEASSESDQLTIKHEENDFS
                     MTLAPILGLSPRALKRFVNVYLLIKVGLSDLQWRIYYEKVHPKTGEKDAVGTIRNFQA
                     VMLLLGIITGLPATSRILFRTLRTKTLRTLGELFEQIDVRKKDDKWYILGTPAEEISI
                     KKKLVDRGLTAVTQNTDSKPINVEVNRYNMEIELLQFTKWLDREKRNWFEVDLDQLRY
                     WDPVVSRYSFRVEPIDQD"
     gene            9278..10144
                     /locus_tag="PLF66_20045"
                     /pseudo
     CDS             9278..10144
                     /locus_tag="PLF66_20045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004755177.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YHYH protein"
     gene            10170..10700
                     /locus_tag="PLF66_20050"
     CDS             10170..10700
                     /locus_tag="PLF66_20050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004422661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66393.1"
                     /translation="MAAFLIGCYDFNTVENTDKDLSIVNGLVFYKGKPFTGKLKQEIL
                     PMNEIHISEYKDGAEDGEYTAKKKDGSLLERRYFKAGVKEGIHRSWFPNGKDRLYSEF
                     KNGTYINDRWEWHDNGVPALYERYNENGNILVTKKWRRTGQIYMNLSFLENGGSIGLP
                     GSKICEPIKSTIKKGE"
     gene            10705..11388
                     /locus_tag="PLF66_20055"
     CDS             10705..11388
                     /locus_tag="PLF66_20055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004445038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HMZ66394.1"
                     /translation="MRKFLIFLILNIFLFCQDDKPKFNTELTENFTPIEGVLPYFNKE
                     VMDPFWSKDGKLPESLRRLTGFTLLTDSGEAITDDKIIGKDKLLVFFYAKCKGICPMI
                     TRNMIDFLPKIKDKSNLLVISISVNPEEDTVEALKKYKAQYRITDNQWVFLTGAKDTI
                     YELARKQFNADVQVVQGTQNLNDFVHTEHIYLIDKNNYLRGFYRAKGTGDLERLLIEL
                     ETLQKEEKK"
     gene            11395..12150
                     /locus_tag="PLF66_20060"
     CDS             11395..12150
                     /locus_tag="PLF66_20060"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HMZ66395.1"
                     /translation="MKNLLEIENLSVKIKNNLILKNINIILESEKITGLIGGSGSGKT
                     TLIKTLLSIHNLEQVEMSGTIKIEGLKMNSKNQKWIQPVFQDPNHYFNPNWNLRQCLE
                     EPLVLNQTVPKEKHREKILSYLNEFSISSDLLEQQITKFSGGELQRISIIRAILSEPK
                     ILLMDEPVSGLDPLIQRDAITLIQRLNKEKKITLLLISHDIDFISELCDFIYVIQEGQ
                     IVEANSCYNILNDPKSEYTKLILNSRNLENIKN"
     gene            12315..14891
                     /gene="clpB"
                     /locus_tag="PLF66_20065"
     CDS             12315..14891
                     /gene="clpB"
                     /locus_tag="PLF66_20065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010577841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HMZ66396.1"
                     /translation="MKLDKITRKLSEAFTSSIQIAEEYKNAEQTEDHLIYSILNDDSG
                     MAEIILSHLNLDRIKFRNLARQSITKLPRVQGSANLEPSRGLLSILKKADSIRADLKD
                     EFLSTEHVLAAFLSESSTQLKNDFIREGLNYNKLLTTIKDLRKGKTVMDDSPESKIDT
                     LKKYARDLNEQARKGKLDPVIGRDEEIRRIIQVLSRRTKNNPMIIGDPGVGKTAIVEG
                     LAGKIIAGDVPDGIKNKSIFALDLGAMIAGAKYRGEFEDRLKALLDEVKNSDGNIILF
                     VDEIHTLVGAGATEGALDASNMLKPALARGELRCIGATTLKEYQKYIEKDAALERRFQ
                     PVFAKEPTIEETIMILRGLKSNYELHHGIRIKDSAIIAAANLSNRYITDRFLPDKAID
                     LIDEASSKMRIEMDSMPENLDRMQKKIQSLKIEREALKKEKDQASISRLKELEEELSK
                     NEEDFSILQSRWKSEREKIQRIKLLKEEFEKYKNLEKEAERNGNLNLVAEIRYGKLIE
                     IDKQLKLANEELSAQYGQERLLREEVSEEDIANIVSRWTGIPVSKMLQGEKEKLLHME
                     AVLKQRVVGQDHAISILSESIRRSRAGLSDANRPTGSFLFLGPTGVGKTETAKALASF
                     LFDDPAAIVRIDMSEYMESHSVARLIGAPPGYVGYDEGGQLTEAIRRRPYSIILLDEV
                     EKAHKEVFNIFLQLFDEGRLTDGKGRLVDFKNTVVIMTSNIGTSILEEDITAEEKERL
                     IEKELKRHFKPEFLNRLDEVVIYNKINESVIEKIVEIQLKELSDRVKQNGIEVSFTNE
                     LKKHLSEAGYDPEYGARPLKRLIQRELGNSLSEFILKGNYQTGAKLLANYKMGSIELN
                     MI"
     gene            complement(14955..15362)
                     /locus_tag="PLF66_20070"
     CDS             complement(14955..15362)
                     /locus_tag="PLF66_20070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002724821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HMZ66397.1"
                     /translation="MLRKKRGIKQYDMARALGVSPSYLSKIETDSQEPTERFKVNVAK
                     YLKTSVEKLFNEQPVEEIFPEFSTGLTNKLWAKRRELGIKQYDMAKKLKVSTPFLSKV
                     ELGLLEPTEEFKQLASKVFKMEQSELFLGTVQY"
     gene            complement(15974..>16704)
                     /locus_tag="PLF66_20075"
     CDS             complement(15974..>16704)
                     /locus_tag="PLF66_20075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66398.1"
                     /translation="ATAPENQKALSDNYLADEGRLENEARMIRDRYVEQLQAKIWNDE
                     YERISKQSGIEFSKEMGTLTEDQLVDTTPIYTKGGKTYTLGDLKSEYAKVNMKPDTYV
                     PQAKELLYFFNSAILPVSLMSDNADVKNLKSSEKFESQLALWRKNITWSFFVRDIQNM
                     QMNITDQEVKDTYEAGKLYTYSAPSKANPEKREPIPFNQVKDKIKEDLRTSKLKAEMQ
                     KQIEKLKNDYHLTLNSDKLKAGSI"
BASE COUNT         6035 a         2439 c         2959 g         5271 t
ORIGIN      
        1 gtcatatctg ttctatggtc attgtcactt tttcaatcca tattttttgc gatcaaagag
       61 tttggagcaa atccaatttt ttcaatcaat atggtaaaag aaaaaaatgt tgaggaagct
      121 agagaatctc tgggacttct cattgtgttt ttagttatga tttggaaata ctatgaagca
      181 aaaaaacact ctaaagaaca gaaataacta tttcgcctag ataattccaa agcggatcat
      241 cagaattttt tagaaagaag ggttctatga tagaagtatt gcagaataaa tcaatagcat
      301 ggcatagcta gggttataga gaacaaaaaa aacgatgggt gaaatgccca ccgtctcttt
      361 tttttctaaa atggaatcaa ttcagaatac tctcagagtc agacaaataa gattgagact
      421 taaaatagca ttttaaaaat aaacgtcatt aattttcaaa aaataaaata tcacttgcag
      481 tttatcaatt tataccatgt gattggtgta tgaaaaaact acttctctcc tttttagttt
      541 taatatttct agtcctctct tatactattt tgttttcgga aaaaggtata tcgataaaga
      601 aacaagcgga taatgataat ttgccaaatt ttgatcaaat tgcagaggaa gcagccttag
      661 acttacaaaa gtacattcaa attaaaacta ttagaggcaa tgaattagaa tctgcattat
      721 tcttaaaaat aatattagaa aaatataata ttccttctaa aatcattaca cctcctaatg
      781 aaccatctag agcaaattta tttgcggaac ttcctggaac agaagaagaa ggtggagtaa
      841 ttttcatgaa tcatatggat gttgtggaag cagaagcagt tgaatggaaa tatcctcctt
      901 ttaatggaat tcgaattaaa gaccgaatct atggaagagg tgcggttgat atgaaagggt
      961 taggtatcat gcaactctac gcaatgttac tgataaaaaa atcaggaatc aaattaaaac
     1021 ataagttaat gtttgctgca ctcgctgatg aagagagtag atcggctaag ggtgctcagt
     1081 ttattgtaaa agaacataaa gagctattta aaggttatga atacattcat aatgaaggtg
     1141 gaacgggaac tgatagtgtt gcggttccag ggagtaaaat atttaatttg caatatgctg
     1201 aaaaaggaat cctatggcta gaattagaag caaaagcaga gtcaggacat ggaagcacac
     1261 cgcctatcgc atatgcagct aaaaacatgg tcaattttct aactgaatta caggaatttt
     1321 ccaaagaaac aatcataagc gatatcactg ctgctttttt ttatcaaatg ggagaggcta
     1381 gtccatttcc aaactctttt gtattgaaaa gatctagaaa tcctatcatt caattagctc
     1441 tcaataaagt aatcaattca aatagacatt taagagccat gacttctaat accgtgagta
     1501 tcacagggat agattctgat cctattggta ttaacgtaat cacaaaccat acaaaaggaa
     1561 gtgttgacat ccgattatta ccaggcgtag atccaaatga ttatttaaat aaaatcagaa
     1621 agattgcaga aaaatataaa atagctatta gcgtaaagca ttccgaagca gcgagtgttt
     1681 cccctatgga tagtaaattc tttcagatac tagcaggcac tgctgtaaaa gttatcccaa
     1741 atgcaatcgt cacacctttt ttatcaccag gaactaccga ttcttcctat tttcgtctga
     1801 atggatttaa atgttatgga ttgattccag ccttacttac agatgtcgag ttggacggaa
     1861 ttcatggaaa agatgaaagt atgagagtag gacatttaaa aactggtata caaattttat
     1921 atgagactat aattaattat aataagttat gaattcaatt ttattttcct attatgcgcc
     1981 tggtgttttc tttgatacgt taatcgcttt attgatttct atttttttaa tttttaaaaa
     2041 agagaaatct atagcgacct attggctgat tggttttttt cttggagagt tctctttatt
     2101 cgcagcctat actgcctctt attccatttt taatgaagtg ggagcatatc atagatattt
     2161 ttcttgctta gtgatgtttg gaaatgctag ccttgtaggt ttttcctact tttaccagaa
     2221 aaacgataaa cccaaagaag gaatgattgt catatcactt tcttttatca tcgcgttggt
     2281 agcatatatc catttcattt taaaaactat ttctatggag aaaatataca atccagaagc
     2341 tcatatctat acatttgatt ttgggaaaga agtcggaatt gtaattgctc tattatttct
     2401 agtttcactc attaatttaa ttcgtaaaac aatacgatat tcgacacatt ctgatttttt
     2461 tggtaagtgg actataaaac cgcaaagatt aacaggatat ccagtctatt atctaaaatc
     2521 attttttttg ggaatatata aaatatattc tcctcgggga aaagaagccg aatccactcg
     2581 agattttgca attccaattt ttttacagat tttaatagca ttgctaaatg tattagtaaa
     2641 atcaggaaag atttcctatg agttgtatgc tcttttattt tcattttcta caatgattgt
     2701 aatttataat attgcgattg tttatttgaa tcattcacca gaaccaactt cttttatggt
     2761 gaagttagtc ggtatatctt taggtgccat attagtagtc cttgcgattg ttggtcaaat
     2821 cagtttaaat gatcgtgagg aaaattacaa tacaattcat ttattaaaaa tcaaccaagc
     2881 taagattgca ttgaataaag gaaattggga agatattcct agcgatgttg aatatattct
     2941 tatgaaacca aagaactctt cttttttttc taaggataca accattcttt tgttaagaga
     3001 tcctgctgta acgttagaag atatttataa agaagtagat aaagagaaag agaaaaccat
     3061 tcaagctaaa atttcagcta ataaagataa aacattatct accgaacaaa aatatcaaat
     3121 agctctagaa gagattaatt catattatcc acccgaacaa aaaagactct atagacaaat
     3181 aaatcataag ttgtttacac aatttgattt tgtggaagaa gaaaatagat atgaaatagg
     3241 atttaatcta agggtttata gagaatatat ccattccaca tccaaaaata ttatcataac
     3301 tatgatttca gtttttttct ccgttttagc atttttccca atgcttttta aaaccagttt
     3361 agtaagtcct cttagaaaac ttttagatgg tgtaaaaaat gttaacgatg gagatttatc
     3421 agttcaggtg cctgtaaaat cctatgatga gattggtttt cttgcagtgt ccttcaattc
     3481 aatggtaaaa tcaatacgtc aagcgagaga agatctgcaa gattatgcag aaaatcttga
     3541 attaagagtg gatgagagaa caagagaagt aagagagaaa atggaactca ttgaatccct
     3601 aaaaatacaa caggatgcag attatttttt aacttctctt ttggctaaac ctctctttta
     3661 taacgcaaat aaatccacat tggtaaaaac tgaatttata attaaacaaa aaaagcagtt
     3721 tgagtttaaa aataaacgag ctgaattggg aggagatatt tgtattactg ggaatcttag
     3781 actcggaaca aaagagaatt acaaacgata tacgatggcg atgaatgggg atgcaatggg
     3841 taaatctatg cagggagccg gcggatcctt agtgatgggc gtcatgatga actcaatcat
     3901 ggcacgctcc gcatctcaaa ataaaattct aaatcagaca ccagaagaat ggttgcgctt
     3961 ggtttatcag gaacttcatt ctgtctttaa atcattcaac ggtagtatgg ttatttcagc
     4021 ggttatcttt attattgaag atgaaacagg taaaggatat tttattaacg ctgaacatcc
     4081 attctctgtg ctctacagag acggtcgagc tagttttatc gaaaaggaat tacaacttag
     4141 aaaaatcggt ttcgatatag atactgaatt taaatatgaa acaattcaat tacaagaaag
     4201 agacgttatc attttaggtt cagatgggag agatgatatt gatttaactc cgaaagaaag
     4261 tgttagaact attaatgaag atgaaacaat atttttacaa ttagtagaaa aggcagagtc
     4321 tgacatttct aaaattgtag atctgatagg agaagttggt actattatag atgatttatc
     4381 gcttcttcga attggttaca aagaggaaat tgttgaaatt gaaaaagtga aagaaccgga
     4441 agtcttttcg gaatctgata taacgattca agttgataaa gatggattta caaaaaccag
     4501 aacggatatc catacaaaca tagctctcaa tactgataat gcatatctcg aaggaaaagc
     4561 gttaagcgaa aaaggtagaa tagatgaagc aatcaatatt ctgtattcag cttttacact
     4621 tgataagacg cattctgaat tgactcgatt gttaggtgtt ctagctttta aagctaagga
     4681 ttataaaaaa gctatagagg cactaaaaac ctatacatct cattttgaaa gagaagagga
     4741 attattgtat agccttgcaa tttcatgcaa aaaaatggag caatataagg attccctttc
     4801 ggtttcaact ttgttagaga gaattaatcc aaaattaatt cgaaatttac tgaacctagc
     4861 tgatgcaaat cgccttattg gaaattttca aaaagcaaag gagttttcag aaagagtgat
     4921 tagtatggac ccaaaaaatg aaattgcttt gaaaattctt aaaattgtta gttaataaat
     4981 ctttatcatc tagcaaaaat tcagaaaagc aaattacgat tgtcaaaaca atttacattg
     5041 tagaaggaaa gatgctctct acaaagatat ataagtttta aaaattttct tgtaatttac
     5101 tgagctaacc gacagaaaga aatcatgagt gatacaatta tatcttccgt ttttatctgc
     5161 cctaaatgta aatatacaaa ccctagcaaa tctaagtttt gtatgcaatg tggtagccca
     5221 ttgtcagatg ctacaaagca aacaaaaaag cctaaactaa ttttattaaa tgattcaaaa
     5281 gtggaaaaag aattcacgat tgaatctaaa tctatcattg ggaaaggtgg agacatctct
     5341 gtagatggtg caaacttcaa agacaattta gcggaattga aattagatgg agatcgtcta
     5401 gttttaactg caatgtcgaa tgatgttttt atgcagattg ctgcaaacaa gccattagaa
     5461 ttagcaaatg gaacaatctt aaaaatcggt gataaagttt ttaaggttga tctacaatga
     5521 atgtattttg tcctaattgt ggaactgaaa accccagcat tgcgagtttt tgtgctaatt
     5581 gtggatttaa tattaaatca ctcactaagc aaagtgatat tgaaatttta agagaagcac
     5641 tttctccaaa agaatacaaa gtagaaagaa aaatcagcgg agggagtcag tcggatcctt
     5701 atctgtgcga gtatttatcc acaggtgaaa aatatgtgat tcggattcta aaaccgaatc
     5761 aatcagacga agttagaaat ttatttgtca ctggcttaca gcttaacgca cagctagacc
     5821 atccaaatat tttaaagatt aaagagatag gcgaaatcaa tggtctgccg tattttattt
     5881 ctaattatgc ggataatggc tctttgtctt gggtattaaa aacatacgga acaaatggga
     5941 tgcctattga agaggcaatt aaatacataa tcaaagtctt aaaaggttta gattatattc
     6001 acacaagaaa cgttatacat cagaatatca aaccggatgc agtttttatt acaaagggag
     6061 gtgagccagt cattggggaa tttgtaatgg caaaaaaggt agaagaggga caaaaagtta
     6121 aaaccgaaag tttggttgga actttggagt atatgagtcc tgaaagatgt aagcttgcaa
     6181 ggagtataga tcgaagaagt gatatctatt ccgttggaat catgctttat caactcctga
     6241 ctggtgaatt gccattcacg ggagatacac aaactctcat ttacaaacaa acaaatgaaa
     6301 agcttcccaa cattacctat aaattattag caaaaggttt aaattctatt attgcaggta
     6361 aagaaaaatt atacttagct gctggagaaa ttcaaagtat tttagaaaaa gcctgtgcaa
     6421 aaggaaagag tagtcgtttt aataccgctt tagaatttgc aactgtattg gaaaagtttc
     6481 ttgagtttgt tagtatccca actgttacaa ataaaaaaga aaatagatgg aaagctttaa
     6541 ataattatag agcagatcat aatgcaaata acagtgatga taggcttggt atcacgaatg
     6601 ctgtagatgc ttttgctagg ttaatttcct ctaaatcagt tcatcctcca ttatcaattg
     6661 gtctttttgg aaattgggga gcaggaaaaa gtttttttat gaataagctc accaaagaaa
     6721 ttgatcgact aacagaaagt atgcgatcag cggcaaaaaa tggagttcct gctaaggatt
     6781 taacctttta cgaaaacata gttcagatcg aatttaatac ttggcatttt gtggatgcaa
     6841 atctttgggc aagtcttgtg aaccatattt tttcaaatct aaaaataaaa ggcgaaccac
     6901 tcgatgaagt aagagaaaga agaaatttct taattaaaag aatagagagt gaaaaaaacg
     6961 gacttttaga tttagaaaaa gatgaagaaa aattttccaa ccaaattgaa gatattaaac
     7021 tagaaatcga atcggaatct aaaaattata aaacaatagt cgctagttta tttgataagc
     7081 attttgatga ggctgttaaa gaaaaattta aaccagatga aattaaactt tgggctaaaa
     7141 gagctggggt agaatacaat cctgatgaac cgataggcgc tcaaatgcat cgaatttatc
     7201 aagaaacaga taatctaaaa aatctaccta tatttgaaaa aataaagata cagtttaaag
     7261 gatattttta ctttgctgtt atcgcattta ttcttttctt aattggttta atttgttcca
     7321 tcgggtttta tatttattct gaaactttta ttggagtttg gatccagtcc tttgcattta
     7381 acacaaaaga gtggatactt ggtgttattg gaagtattac tgcaatgtta tctccattac
     7441 tcccaattta ttatggactc aaatcttggt cacaaaagta tttaaactgg ggtcctaatt
     7501 ttttaaaaaa gattaaggat acaattccag acttgaacaa agagttggaa aataaagaaa
     7561 aagaaaaaca agagaagttt ttagaattag aaaataagaa aaaatccttt gagaccaaaa
     7621 aagaaaatgc aagattagaa attcaaaaac tagaatctga actagaaaaa acatccgatg
     7681 gagattattt atcaacattc atacgaaatc gtatgggaac agatgaatac acaaaacatt
     7741 taggtttaca ggcaaagatt cgtaaagact ttgaacagct ttcaagttta attgataaat
     7801 acaataaaag catacttgca ggagaagtga aagacaatga tggttatatg atcaatcgaa
     7861 ttgttttgta tatcgatgat ttagatcgtt gtccgcctga taaggtagtt gaggttttac
     7921 aagctgttca ccttctactt tcttttcccg cctttgtcgt tgtggttgct gtagatgcaa
     7981 gatgggtttc tcaatctcta cgaattggat atagagatct ttttggagag gaaatttctt
     8041 tagatacgga tggagatggg atacctgata gctttcgagc gactccacat gattatttgg
     8101 aaaagatctt tcagattcca ttttggcttt atccaatgaa tatcgaaggt aggcgtaatt
     8161 tactagccca attgatggag agcaatctag aggcaactaa cgatttgccg caagagccgg
     8221 tgctagaaga taaaactaat ttaatagaaa ataaaacaaa tctaacaaac aatgaaaagg
     8281 aaatagagca aagggaagac cctggtttta aaacagatat tctaaaagag gatgaaagta
     8341 ttctagaaga aaatgaatat actttcaaac tagaagaagc ttccagtgaa tccgatcaat
     8401 tgaccattaa acatgaagaa aacgatttct ccatgactct cgcgccaatc ttaggtttat
     8461 ccccaagggc tcttaagaga tttgtgaatg tttatttatt aataaaagta gggctttctg
     8521 atttgcagtg gagaatttac tatgaaaaag tgcatcccaa aacaggggaa aaagatgcag
     8581 tcggaaccat acgaaatttc caagctgtga tgttattact tggaattatt acaggtcttc
     8641 ccgcaacttc tagaattctt tttagaacct taagaactaa aactcttagg actttaggtg
     8701 agcttttcga acaaatcgat gtaagaaaaa aagacgacaa gtggtatatc ttgggaactc
     8761 ctgcagaaga aatttcaatt aagaagaaat tagtggatcg tgggctaacg gcggttacac
     8821 aaaacaccga ttccaaacct atcaatgtgg aagtaaatag atacaatatg gaaatagaac
     8881 ttttacaatt tacgaaatgg ttagatagag aaaaaagaaa ttggtttgaa gttgatttag
     8941 accagttgcg ctactgggat cctgtagtat ctagatattc ttttagagtg gagccgattg
     9001 atcaagatta atttttattt taaaaagaac aatcctataa ggttggtcta gaaaattaca
     9061 tgatttttat tttcattttc aaatccactt gactcttttt ggggttaaca ctatgttatg
     9121 tgaatatatt tttcggagaa tacaaatgat taaacataaa acagcaacat taatttcatt
     9181 cttagtttta actagcatta gtttttactt ttcttgtaaa caattaggac tggaaaaaaa
     9241 cgattcaaac aataatacag ccattgcagc cgtgctttta tctgcaagtt ctgcagcttc
     9301 ctcgagttca agtagttgta atagcggaac ctctacaggt tcaacaatca cactgactag
     9361 tgcgacttta gatgcaacaa ctgactgtgt aagtggagtt acaacttgta tggatagtgc
     9421 actgcctact tggataaaaa ataattttaa atgtgcggtt ggctatgttt caggaacaaa
     9481 ttatgttttt aaatcaaaaa atttaccaaa tactaagagt tattattata ctcaatatac
     9541 aaatcaaact cctagcacaa gttattcttt atatgaagca ttgcctagcg gaaatacgaa
     9601 agcaggtact aatacagtta ttagccaaaa ctttgtgtat acaattcctg ctacaccaac
     9661 gagagctacc gttagttctg gaacacaggc gggtttgatt tcaattggaa taactagaaa
     9721 tggtttggca atctttaaca atgctgcagc tccgccagat accttagctg tagaggctgg
     9781 aacatttgat aattaccaag gacatcccca aaattctgga atttatcatc atcacgcagc
     9841 agttccaaag atttgtgatg gtgggatcaa tacaagtaat ggtaatgcat gtaaaaattc
     9901 ttctctaatt ggaattgctc tagatggtta tgcaatttat ggtcaatatt gtgataaagg
     9961 tacagcttct actgcagatg atacagctcc aacagattta gattctaatc atgggcatac
    10021 agcagttaca acgcatttca gcacagcgac ttatcattat cattatgcct atgactctac
    10081 agcaaccata tatactttga tgggttcata tttttatggt aatattggct cagcgacaaa
    10141 ttaatattaa ccgcttagtt tcgattttta tggcagcctt tctaataggc tgctatgatt
    10201 ttaatacagt agaaaatacg gataaagatt tatcaatcgt gaatggtcta gtattctata
    10261 aaggaaaacc tttcacgggt aagttaaaac aagaaattct tcctatgaac gaaattcata
    10321 tttctgaata caaagatggt gcagaagatg gagaatatac tgcaaagaaa aaagatggga
    10381 gtcttctaga aagaagatac ttcaaagcag gtgttaaaga aggaattcat cgctcttggt
    10441 ttccaaatgg aaaagataga ctctattctg aatttaaaaa tggaacctat ataaacgata
    10501 ggtgggaatg gcacgataat ggagttccag ctctttatga aagatacaat gaaaatggaa
    10561 atattttagt caccaagaaa tggagaagaa ctggacaaat ctatatgaat ctctcttttt
    10621 tggaaaatgg tggatcgatt ggactaccag gtagtaagat ttgtgagcca attaaatcga
    10681 ctataaaaaa aggagaataa tcagttgaga aaatttttaa tctttctaat cctaaatatt
    10741 tttttatttt gtcaggatga taaacctaaa tttaatactg aattaactga aaattttact
    10801 ccaatagaag gagttctacc gtattttaat aaagaagtga tggatccttt ctggtcaaaa
    10861 gatggaaaac ttcctgaatc acttaggcgg ctaacaggct ttaccctcct tacagactcg
    10921 ggtgaagcaa tcactgatga taaaataatt ggcaaagata aattgctggt attcttttat
    10981 gcaaagtgta aaggaatttg cccaatgata actcgtaata tgatagattt tttacccaaa
    11041 attaaagata aatctaattt acttgttatt tctatttctg taaacccaga agaagataca
    11101 gttgaagctt taaagaaata caaagcgcag tatcgcataa cggataatca atgggttttt
    11161 ctaacaggag ccaaagatac tatatatgaa ctagccagaa agcaattcaa tgcagatgtt
    11221 caagttgttc aaggcactca aaatctaaat gattttgttc atacagagca tatttactta
    11281 atagataaaa ataattattt aagaggattt tatcgagcaa aaggaaccgg agatttagaa
    11341 aggcttttaa tagaactgga aacattacaa aaagaagaaa aaaaataatc taatatgaaa
    11401 aatcttttag agattgaaaa tttatccgta aaaatcaaaa ataatcttat cctaaagaat
    11461 ataaacatta tcttggagtc agaaaagata accggactta taggtggttc tgggtctgga
    11521 aagacaaccc tcattaaaac acttcttagc attcataatt tagaacaagt agagatgtca
    11581 ggcactataa aaatagaggg tcttaaaatg aattctaaaa atcaaaaatg gattcaacca
    11641 gtgttccaag atccgaatca ttatttcaat ccgaattgga atttaagaca atgtttagaa
    11701 gaaccgttag tcttaaatca aactgttcca aaggaaaaac atagagagaa gatactttcc
    11761 tatttaaatg agttttccat ctctagtgac ttattagaac aacagattac aaaattttct
    11821 ggtggtgaat tgcaaagaat ttctattatc agagcgatac tttctgaacc gaaaattcta
    11881 ttgatggacg aaccagtcag tggacttgac ccattgatac aaagagatgc aatcacactt
    11941 atacaaagat taaataaaga aaaaaaaata acacttctac ttatatcaca cgatatagat
    12001 tttatttcag aactttgtga ttttatttat gtaattcaag aaggtcaaat tgtagaggca
    12061 aatagttgtt ataatatctt aaatgatcct aagtcggagt acactaaact aattttaaat
    12121 tctagaaatt tagagaatat taaaaattaa aatctttttt aaaagtcatc tttgctattt
    12181 tttttgatat tcagtttcaa aaaatatttt tatatatttc ttttctttat aaaaaatatt
    12241 ttagtttaaa tcctcatttt aactatactt aaattatgat tgaaattaag taaaaccgtc
    12301 taataagtaa gaatatgaag ttagacaaaa taacaagaaa actatctgag gcattcacta
    12361 gttccatcca aattgcagaa gagtataaaa atgcagaaca aacagaggat catttaatct
    12421 attctatatt aaatgatgat tctggtatgg cagaaattat tctatctcat ttgaatttgg
    12481 atagaattaa atttagaaat ttggccagac aaagtattac taaattacca agagtgcaag
    12541 gctctgcgaa tctagaacct tctcgtggtt tattaagtat actcaaaaaa gcagacagta
    12601 ttcgtgctga tttgaaagat gagtttctct ctacagaaca tgtcttggct gcttttttat
    12661 ctgagagttc tactcaatta aaaaatgatt ttatacgaga aggcctcaat tataataaat
    12721 tattaacaac cataaaggat ttaagaaaag gtaaaacagt tatggacgat tcaccagaaa
    12781 gtaaaattga tacattaaaa aaatatgcaa gagatttaaa tgagcaagct agaaaaggaa
    12841 agttagatcc agtcatcgga agagatgaag aaatacgtcg aattatacag gttctatcca
    12901 gaagaactaa aaataatcca atgattattg gagatcctgg agtagggaag actgcaatag
    12961 ttgaaggtct cgcaggaaaa atcatagcgg gtgacgttcc tgatggaata aaaaataaat
    13021 ctatttttgc tcttgattta ggagcaatga ttgcaggcgc gaaatataga ggagagtttg
    13081 aagatagatt aaaagcctta ttggatgaag ttaaaaattc agacggaaat attattctat
    13141 ttgtagatga aatacacaca ctagttggag caggagcaac cgaaggagcg ttagatgcat
    13201 cgaacatgct aaaaccagcc cttgctcgag gggagcttcg ctgtatagga gcaactaccc
    13261 taaaagaata ccaaaagtat attgaaaaag atgctgctct agaaagaaga tttcaaccgg
    13321 tatttgctaa agaacctacg attgaagaaa caatcatgat tctacgtgga ctcaaaagta
    13381 attatgaatt acaccatgga attcgaatca aggattcagc aattattgct gcggcaaacc
    13441 tttccaatcg atacattaca gatcgctttt taccagataa agccattgat ttgatcgatg
    13501 aagcttctag caagatgcga attgaaatgg atagtatgcc agaaaactta gatcgcatgc
    13561 aaaaaaaaat acagtcttta aaaattgaaa gagaagcact taaaaaagaa aaagatcaag
    13621 cctctatatc tagactaaaa gaattagaag aggaactttc aaagaatgaa gaagattttt
    13681 ctatcttaca aagtaggtgg aaaagtgaac gagagaaaat tcaaagaatt aagctcttaa
    13741 aagaagaatt tgaaaagtat aaaaatttag aaaaagaagc cgagcgaaat ggaaatttaa
    13801 atctagttgc tgagattaga tatggtaaat tgatcgaaat tgataagcaa ctaaaattag
    13861 ccaacgaaga attatcggca cagtatggac aagaaagatt acttcgagaa gaagtttccg
    13921 aagaggatat tgcgaatatc gtttctcgtt ggactggaat tcctgtttcc aaaatgttac
    13981 agggagaaaa agagaagcta ctccatatgg aggctgtctt aaaacaaaga gtcgttgggc
    14041 aagatcatgc tatttctata ctgagcgagt ctattcgtag atcgagagct ggactaagtg
    14101 atgcgaatcg accaacagga tcctttttat ttttaggtcc taccggcgta ggtaaaaccg
    14161 aaacagcaaa agctttagct agctttttat ttgatgaccc tgctgctatt gttcgaattg
    14221 atatgagtga atatatggaa tctcattctg ttgcgagact cattggcgcg ccacctggat
    14281 atgttggata cgatgaggga ggacaattaa cagaggcaat tcgaagaaga ccttatagta
    14341 tcatattgct tgatgaagta gaaaaagctc acaaagaagt ttttaatatc tttttacagc
    14401 ttttcgatga gggtagactc acagatggaa agggacgttt agtagatttt aaaaatactg
    14461 tagtgatcat gacttcaaat ataggcacat ccattttaga ggaagatata acagcagaag
    14521 agaaagaaag gttaatagaa aaggaactta aaagacattt taaaccagaa tttttaaatc
    14581 gtttagatga agtggttatc tacaataaaa tcaatgaatc ggtgatagag aaaattgtag
    14641 agattcaact caaagaactt tccgaccgtg ttaaacagaa tggtatagaa gtttctttta
    14701 caaacgagct aaaaaaacac ctaagtgaag ctggatatga tcctgaatat ggtgctagac
    14761 cattgaagcg attgattcaa agagaactcg gtaattcatt gagtgaattt atccttaaag
    14821 gcaattacca aacgggggca aaactcttag caaactataa gatgggttct attgaattaa
    14881 atatgattta gaagatagga aacctttccc tttaatttta tgatttatta aagggaatag
    14941 gttcactttt gactttagta ttgaactgtt cctagaaata attctgattg ttccatttta
    15001 aaaactttgg aagctaattg tttgaattct tcggttggtt ctaaaagtcc taattccact
    15061 ttagatagaa aaggtgtaga aacttttaat ttcttagcca tatcgtattg tttaattcct
    15121 aattctcttc tcttagccca aagtttattg gttagacctg tgctaaattc aggaaatatt
    15181 tcctcaactg gttgttcatt gaaaagcttt tctactgatg tttttaaata ctttgcaaca
    15241 tttactttaa atctttctgt tggttcttga ctatctgttt cgattttgga gaggtagctt
    15301 ggtgatacgc caagtgctct tgccatatca tattgcttaa ttcctctttt tttcctgagc
    15361 atataaatgt gatttttcat ctatgtcacc tcttagtgca cttataacag acttattcca
    15421 taacacaaca atttatttaa taaataataa ttttttgatt aaaaaaaatt atttcctaat
    15481 tgcattatgc acaaatctat ctgagcctcc aaaattgatg tatttctgat accataaaaa
    15541 aagaattatc tctgctttca ctttaacaat ttttaatatt taagaaagag gcctctttta
    15601 ttattagcac ccaaacctag tgtttcaatt tggtttaaga attagaattt ataattgtta
    15661 aaaatttcaa gttcttccaa tgatttcaat tttttgtaaa gtatggtgtg tgaagaaata
    15721 gtgaaattca caaatctagt ttctagatta tagtctcaat atttctgaca caataaaaat
    15781 tatttttctt aaatcaaaat tagattcaac aaaagatttt aaaaaaagga atcagaggaa
    15841 ttctatagaa tgaaaaattt gatttatagg atatagaagg attcgctgta aaaagttatg
    15901 ctgattcgaa atttttttat cgtaagaagt aggaagagta tattagctct tactaatata
    15961 ctctttttaa atgttaaata gatccagctt ttaatttatc tgaatttaat gtgaggtgat
    16021 aatcattttt taatttttca atttgttttt gcatttctgc ttttaacttt gaagttctta
    16081 aatcctcttt aattttatcc tttacttggt taaatggtat aggctctctt ttttcaggat
    16141 tagctttgga gggggctgag tatgtatata attttccggc ttcatacgta tctttcactt
    16201 cctgatctgt aatattcatt tgcatatttt gaatatctct tacaaaaaaa gaccaagtga
    16261 tattttttct ccacagtgct agctgacttt caaatttctc agaagatttt aaattcttaa
    16321 cgtctgcgtt atcactcata agactcacgg gtagaatagc agaattaaaa aaatacaata
    16381 actcttttgc ttgcggcaca tatgtatccg gtttcatatt aacctttgca tattcactct
    16441 ttaaatctcc tagtgtatag gtctttccac ctttggtata aattggtgtt gtatccacta
    16501 gttgatcttc tgtaagagtg cccatttctt ttgaaaactc aattccactt tgttttgaga
    16561 ttctttcata ctcatcattc caaatcttag cttgtagctg ttctacatat cgatctctaa
    16621 tcattctagc ttcattttca aggcgacctt catcagctag ataattatcg cttaatgctt
    16681 tttggttttc tggtgcggtt gctt
//
LOCUS       DAOQOS010000117        16682 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_1734_length_16682_cov_5.524268, whole genome shotgun sequence.
ACCESSION   DAOQOS010000117 DAOQOS010000000
VERSION     DAOQOS010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 16682)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16682)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16682
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1734_length_16682_cov_5.524268"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1406)
                     /locus_tag="PLF66_20080"
     CDS             complement(<1..1406)
                     /locus_tag="PLF66_20080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HMZ66399.1"
                     /translation="MKQILTIILMFFYLISCSSTDEKESSNEIKKPVKSSQKKLHITP
                     KEYEVIKKLKLKPGLLETDSGSIMQISFSIDTFVNKVFKIDKKVYYRGTQIQFRNAYT
                     DAGFQNNDKQVVTDAMVNELALLLSNCDSQKYCNDTLVDIDNDGIIDYIPLPKKSDSM
                     SCYVHLNSDGTQNYIECNYQDKESRTIKRSIDDNENGYPNQFIYYDPQKETKTNLCIT
                     KIDYDANNNRRLDKWDYYENCKLVRREIDENENGTVEEITYYNKKGEYDRFWSIGGDN
                     VKKANDALEAKKYDEAISFYKAANQELSKEWGADSNEVCGNLNRIGETYNAAQKFPDA
                     IKTFNQGLALKKCSDWNYSHLSSGLLLTYAFMDDIKMIIKSGIDAAQQYAKTNEGKKH
                     FEIEGLLGYGYYKEKEYQEAYKHLIPALELTLQEQKESCKGEKQCSYFNPFYFNTFGN
                     ICSRLNKNAEGIAYYKKA"
     gene            1710..2801
                     /locus_tag="PLF66_20085"
     CDS             1710..2801
                     /locus_tag="PLF66_20085"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HMZ66400.1"
                     /translation="MVSLIELLRNKYGEKFPKYISNRQLIEFLNESKDLIEKENYLEL
                     VNFYFKHAQTRRSLFREWEIENRQLLDYKEKIKKAKKEFPQEKEFRELLAIRQYSRKT
                     IKAYTQALRTANEYLQNSHNVLISEANREQFYEYFLFLTKERKVSTSTLRIARFSIEY
                     YRSELIFQPVTLDFAYQLRKKEHLPTIFSKSEIIKILNSTNNLKHKLVLSLLYSAGLR
                     LSEVIRLKVKDIDLSRKTIFIREGKGGKDRITVLSDKLVTDLSIFMENRIGKDILFPS
                     NQKSDLGNEKPLSARTVQKILSTALLKSGIRKKGTPHDLRHSFATHLLETGTDIRFIQ
                     RLLGHKNISTTTIYTKLANPNIAGVKSPL"
     gene            complement(3092..4405)
                     /locus_tag="PLF66_20090"
     CDS             complement(3092..4405)
                     /locus_tag="PLF66_20090"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HMZ66401.1"
                     /translation="MSLKNREKAFLISLQIGIVACAISVSLSQLFLFITVFFFILTKR
                     YKEVRFTPIFFLSIGIFGFYLISILSHTLTSGNFSLAASFKGSEFKDLLLFSAFLVIQ
                     SIKGEEEIRKVEKAFTILLFVLIVTGFISIFTQVRLSRLITDLIKPSPNWKYTHHYGD
                     ILKIGIHLPIGLMNTHLTFAGIFLFFAPYVVFQFLFAVRDRKRVLYYLGILIAFSSIA
                     LLNNARSALFGAGVSILIGFIDLIFVQKYFSLKNTIKLLSIPILLIAILGSLLVFNET
                     TAKTIQPLLGQEKHTDSGRTFIWSSTYPMIFENPVLGIGPGNYGKEVEKVRKNLSEKY
                     TELLFFYEVTQRGHAHNDFLHLAAIAGIPALLFYASLAAAICYYLLRPNQSRTDNLLF
                     YGLIGFFFSGLFQCYFQDDEVVIVFWYLLGYFHFQKFNKSYDANR"
     gene            complement(4389..4742)
                     /locus_tag="PLF66_20095"
     CDS             complement(4389..4742)
                     /locus_tag="PLF66_20095"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HMZ66402.1"
                     /translation="MILLFSSLSKVGSMTDANNEIIVEVTKDFEDLVPEYIDSMNQSL
                     GLLESLLENRNFEELKKEAHKMKGHGGAYGFTYISDMGRLIESFAEEEKGELIRKCLK
                     ELRTYMGRIKIEFKE"
     gene            complement(4952..6838)
                     /locus_tag="PLF66_20100"
     CDS             complement(4952..6838)
                     /locus_tag="PLF66_20100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66403.1"
                     /translation="MSDQGRIHYFFSSNISYQKSFYTFTICLIFSILIGFLKYDTNYP
                     IQNTEIYPLKNGWEYRIGDSPRNEQGVFTFLNDPNLKWSPFLNDSFLKKTEEAKILWI
                     RIQLPSLNLARPGIYFYNVTDVFEAYIDGKIIFNDYPIEKDTDSLTKGLNRHEILSLE
                     KEHLGKTLYLRIASGWKDVGIAGIPLLGNTISLLFYNLKDYALMGISGVAYICFSLIG
                     IYFLYQRPEIKIVFYFSMLSLLSGITTIHHSPFKYLIYNEPMYWTHINTFTILGTIYF
                     FIGYFSEFFENKFQFYKIGKGVILFFILLNTLILFTVKISLVHFYYLERPVVIFVAVM
                     IFYTLYRLYVSASEGNYLAKVLLIGKSILLISLFSFFLHTLGFIPHRGFFFPLGFNGL
                     ILSIAFISVKRYTEIYDIAEEYSKNLMIKNAELSEVNAQLKDLHEFKERIFFDIHDHI
                     GGDISELLILQNQLRNYSVPDSHIEYNVHLLRKINQNVRNKMQMIQDFNNIQENFAMG
                     LSIYLLRRFGSVERKCKLEFQEGLADIFEEFPHSKKQVLFSIATEIANNDLKYGYANS
                     EWIFTSNGSDLFWRVTSNTNYKVGEKSGEGTRGIQNRLKSVQGTFAQSLEGDRIFLEI
                     KIPI"
     gene            complement(7049..7300)
                     /locus_tag="PLF66_20105"
     CDS             complement(7049..7300)
                     /locus_tag="PLF66_20105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66404.1"
                     /translation="MGLIGVNEGKTKAFKNSLISKWKMRLIGSALAFSQLGFEANRVG
                     DFETKEKVKIDWFRKVNFRKVESVSLQRFLFYSKYRSRN"
     gene            7520..8401
                     /locus_tag="PLF66_20110"
     CDS             7520..8401
                     /locus_tag="PLF66_20110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66405.1"
                     /translation="MKNILILILFISFFSIHSEPNSSGNTANPSTDPNAAQPKVIDQS
                     KIVVSVPGPGGGLGDTAWGQSFMSIRDQINKIRQNPEVKEKIEILNESKDKSLLVKRN
                     NITYLYRFYKKPAILEKFNKSKEDEGKSSSLFSVGVYFSPVESLKLKKQVEDKYGNPS
                     TEPQAQIPKDKFSKPDEENDSVGENQDDKKIAGAYTWSFKRADAGNSSAGSNPPPAEG
                     ANANSAPQSNKAEFIIQWVEPYNKKAFSKRMDYFSVDRTDLINIDYKDYFSARETDVL
                     LDLVNEGVFQEKEKDKP"
     gene            8421..9065
                     /gene="fsa"
                     /locus_tag="PLF66_20115"
     CDS             8421..9065
                     /gene="fsa"
                     /locus_tag="PLF66_20115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020771669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HMZ66406.1"
                     /translation="MKLFLDTADTNEISKVADLGLLDGVTTNPSIIASSKRKFKEVIK
                     EICDITKGPVSAEVLSSTYSEMLNEAIDLAKIADNVVIKIPLIPDGIKCVSELTKRNI
                     KTNVTLCFSAPQALLAAKAGATYISPFIGRLDDIAFNGLDLISEIRTIYDNYQFETKI
                     LAASIRHPIHFKEVALEGADAVTLPYATFQMLFKHPQTDLGLAKFLEDAKKIVQ"
     gene            9113..10363
                     /locus_tag="PLF66_20120"
     CDS             9113..10363
                     /locus_tag="PLF66_20120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66407.1"
                     /translation="MMLKKGIAVVFIYFLIWMSYTNTKVFNQTDSIWSIYSTLSLVRE
                     NNLDLDEYKDLFPKAFYANLTQANGHYYNYYPYGVSLLAYPYIWIIDKSYSLNHKDLK
                     EKILSTSTVNLEKTIAASLLALSACILFLLSLKELNSYLNSFLTVFIFAFATLNFSVL
                     SRGLWQHTGSVFLLMLVLYLFHSKKDTLITLSAFPLFFSYVVRPTNLLPILFLGLIAI
                     YQLRFKSIYFLGIGIVILSSFFYTNSVLFGTMSHPYYDFKKVGGSNTFLEAILGNAIS
                     PARGILFYSPVFLFSFYGLYSKIKKEKFQTLDFAIIVLILTHWILVSRNLNWWGGHCF
                     GYRLLSDLIPFFIYYLIYFIKEIDWKVKSPILFLFILSLFISLFINLKGATEMNSYNW
                     NLIPSNIDTDPKRLWDLQDLPFLR"
     gene            complement(10364..11365)
                     /locus_tag="PLF66_20125"
     CDS             complement(10364..11365)
                     /locus_tag="PLF66_20125"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HMZ66408.1"
                     /translation="MANPFNSFNQHKYSIILKNAVLFILFAIAAYILDPLEFVQISHG
                     LFWIVYNTIYIILFLFVNFPELKYLKRLLDLLNYFHTSESHSLDFVNLNLQTFVFPLN
                     LSDENYEVFGKLQPATYLGGDIINHARDNDGNYWFAVGDASGHDLNSHLFSMMILNQM
                     NYLVNVAKTPKDMNAMMNKVLRERIESYPVPVNSYASLGLLKADAKGNFEHYGLHPNF
                     LLYRVDKKDIEVIETSGHFIGIEVPSHIPTNPKEEYNNRNFTMNSGDILFCFTDGLFE
                     QRNKERKYFGYRLYEFIKNEDKSNFPQFAKKLFDSVRTFAGVKQLDDDMTILIIRKK"
     gene            complement(11374..12105)
                     /locus_tag="PLF66_20130"
     CDS             complement(11374..12105)
                     /locus_tag="PLF66_20130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66409.1"
                     /translation="MEKISEYNSSYRFSLFIFTAWIIWRWWWRFYLFPILNTLKEFFY
                     ASKEAIQSLSFGILLLFLFNCSTPWYRTLPNEELPSTRNFFGVWKKKTNSRAAINSSW
                     HKNQWSESIYLRSDFTFTKVYEASDLVGREKHENTVIGKGVFKVNGNWILLETKSIFA
                     EKKLNGVIEKKEERSQESALLYYYSVEGNLIIPMIYDMGYKEKDFGVKDGVSKPFDDK
                     NPNFFQYIKIYAFKEFQSHAYYPEN"
     gene            complement(11997..13412)
                     /locus_tag="PLF66_20135"
     CDS             complement(11997..13412)
                     /locus_tag="PLF66_20135"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004437188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HMZ66410.1"
                     /translation="MLFNSIPFLLLFIVTYLVYWNTEDKYKKYIIAISSFLFYLYYDV
                     LATIHFFSVIGVNYYFSNKMFSLKEKGENTSKLLKIILILNIINLAFFKYFYFLLDSF
                     YSLSHSQMFKDLSTSVNIILPLAISFYTFQLIAIQVDIHKGRVTERIDFFDYTIFIIF
                     FPQLIAGPIMRTSDFLPRLNSPDITPDSMKRGLFLLIVGLFKKVVIADNVGSLIAPVY
                     MDPDKYHFVSIFIAFLGFAAQVYSDFSGYTDMARGLALLLGFEIPENFTGPFYSATFA
                     ELWSRWHITLSTWLRDYLYIPLGGNAKGFHMSNVNMFITMVLGGLWHGANLAFILWGA
                     YFGVILWIERLFFSKGYTLTPKNPILLFCKRMFVFVLFSMSAIFFRSGSYGTQSTTLM
                     YKLTVDFFGMNKGRDIGGRDEVILYIVLALLLNFLQYNHQIVMKWKKFQNIILPIASL
                     FLFLLLGLFGDGGGDFIYFQF"
     gene            complement(13412..14434)
                     /gene="sppA"
                     /locus_tag="PLF66_20140"
     CDS             complement(13412..14434)
                     /gene="sppA"
                     /locus_tag="PLF66_20140"
                     /inference="COORDINATES: protein motif:HMM:TIGR00706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HMZ66411.1"
                     /translation="MIEKKRSEFIGRIGFILGFLLLFSNCIIGNTIKLPSLGQEREAD
                     LIEHTMEGVDKDKILVISIDGVISENPSGGNIFGAGASESTVSAIINDLFKASQDKDI
                     KGIILKINSPGGTVTGSDIIYRELLKFKTLTGIPIVALFMDKATSGGYYVAMAADRIV
                     SLPTAVTGSVGVILSGFNAKEGLDKIGIKDQSITSGANKAIGSPFLEMKPEQKQILQS
                     IVNNLFERFFSIVKTNRQNIQEQRLREVCDGRIFTAEQAFKEGLVDKVGYFEEALFEL
                     MRSEKYARNATPNNNKPRIIYYSHLKQRSRSVYQIMAEETGSSNLLENILKANTEVKF
                     MYLWAY"
     gene            complement(14436..15104)
                     /locus_tag="PLF66_20145"
     CDS             complement(14436..15104)
                     /locus_tag="PLF66_20145"
                     /inference="COORDINATES: protein motif:HMM:NF015961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HMZ66412.1"
                     /translation="MKYIIVSYIAKFILSFWFFFIRKQEYFVPEATEKYLKQKNGFVF
                     AGWHNQILSLTRHVAYYLQRDRHIILTPLVSMSKDGEFIYQTFLRFKMESVRGSTSKG
                     GSTALRQLLKAIKDGRVPIFTPDGPRGPRYKVQTGVIQIASLTKLPIITFYSRFDEYY
                     EFKSWDRQRFPKFFAREWVDYSEPFFVPEKIENYEDYALELEKKMMEQVERLDKIVEA
                     SRNK"
     gene            complement(15152..16432)
                     /locus_tag="PLF66_20150"
     CDS             complement(15152..16432)
                     /locus_tag="PLF66_20150"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HMZ66413.1"
                     /translation="MASEIPLGINPRKMIPYNVFISDDSISERKLIQQFLKSADFKVI
                     GEAEGGEETIHKIKELNGQVDILCVDYSMPEKNGGQVIEEIRTFFPKLIIILITAFSE
                     KEIVEEAVRLKVNAFILKPITKSAIYEKLTFLLGRRDLTNKMVVGYKQMGINLNEIQI
                     PPLRDVMNRVITFDSKKSGGSKELEMIIAPDKALSADILRVANSAYYGRSGSVDTLQN
                     AITLIGLGTIRNIVILQFRKNFTKNLPQPLFRKHLNEIPILTGLIGIDLTGPLNLKNV
                     REQIVISSILRKVGMSILAQNLKLKYLDILKLFEYGSKPLVQLEKEELNIDHIQIGIK
                     VFQMWKLPNSLKKVVANQGFTQEETSRVEDIDRILRMAEILSLSLLGITISEEDSNIL
                     KSIMNYYNVTEELTSLFNEEYYTDIKSHPFFDSI"
BASE COUNT         5676 a         2969 c         2435 g         5602 t
ORIGIN      
        1 aatgcttttt tataataagc tataccttcc gcgtttttat ttagtcgtga gcaaatattt
       61 ccaaatgtat taaaatagaa aggattgaaa taagagcact gcttttctcc tttacaagat
      121 tctttttgtt cctgtaaagt aagctctaat gccggaatca aatgcttgta tgcctcttga
      181 tactcttttt ctttataata gccatatcca agtagtccct caatttcaaa atgtttttta
      241 ccttcatttg tcttagcata ttgttgtgcg gcatcaattc cactcttaat aatcatctta
      301 atgtcatcca taaaagcata agtcaataat aatccactag ataaatggct ataattccaa
      361 tcactacatt tttttaaagc aagaccctga ttgaatgttt taattgcatc tggaaatttt
      421 tgagctgcat tatatgtttc gccgattcga tttagatttc cacatacttc attactatcc
      481 gctccccatt ccttagataa ctcttgatta gccgctttgt aaaaactaat tgcctcatca
      541 tatttctttg cttccaaggc atcattagct ttttttacat tatccccgcc gatgctccaa
      601 aaacgatcgt attcaccctt tttattataa taagtgattt cttctactgt tccattttcg
      661 ttttcatcaa tttctctacg aacaagttta caattttcat agtaatccca tttatctaag
      721 cgacggttat tatttgcgtc gtagtctatc ttagtaatgc aaagattagt cttagtttct
      781 ttttggggat catagtatat gaattgattt ggataaccat tttcattgtc gtcaatggat
      841 ctcttgatgg ttcttgattc tttatcttga tagttacatt caatataatt ttgcgttccg
      901 tcggaattta agtgaacata gcaactcatg ctatcagatt ttttaggtaa gggaatataa
      961 tcaataattc cgtcattatc tatatctacc aatgtatcgt tgcaatattt ctgtgaatca
     1021 cagtttgata gcaataatgc tagttcattt accattgcat cggttactac ttgtttgtca
     1081 ttattttgaa aacctgcatc agtgtatgca tttcgaaatt gaatctgtgt tcctctgtaa
     1141 tatactttct tatctatttt aaagacttta tttacaaatg tatctataga gaaagaaatt
     1201 tgcattatag aacctgagtc agtttctaat aaacctggtt tcaattttaa tttttttatt
     1261 acttcatatt ccttaggagt aatatgaagt tttttttgag aactttttac tggcttttta
     1321 atctcattag aactttcttt ttcatccgtg ctagagcagc ttataagata aaaaaacatt
     1381 agtataatcg ttagtatttg tttcattcgt aatttctcct taactccact ctaagttttt
     1441 ccgagacacg atgtcgagga aacctacatc tggatatttc agcgtcagtt cctcttcagg
     1501 atatggtcga acctaaaatg ccgtctaact aaacttatct ctattacacc taccatgtca
     1561 agaggaaaag aatacaaaaa gtgctttggg aaagtaaaaa gcgaattaaa agtgcggata
     1621 tgctgaatag gggaaattta atgagttaag aatttagaaa gaaaataaat actatacaga
     1681 tattacgtat atgaaataat tggacggata tggttagttt aattgagtta ttgcgaaata
     1741 aatatggaga aaaatttcca aagtatattt caaatagaca gctaattgaa tttctaaatg
     1801 agagtaagga tcttatagaa aaggaaaatt atttagaatt agtgaacttt tattttaaac
     1861 atgcacagac acgaagaagt ttatttaggg agtgggaaat agagaatagg cagttactag
     1921 attacaaaga aaaaattaag aaagcaaaaa aagagtttcc tcaagaaaag gaatttaggg
     1981 aactacttgc gattcgacag tattccagaa agacgattaa agcttataca caagcgctcc
     2041 ggactgcaaa tgagtatttg cagaattcac ataacgtttt gatttcagag gcgaatcgag
     2101 aacaattcta tgaatatttt ttgtttctta caaaagaaag aaaagtttct acctcaactc
     2161 ttcgtattgc tcggttttct attgaatact accgaagcga acttatcttt caaccggtta
     2221 cacttgattt tgcctatcag cttcgaaaga aggagcattt accaactatt ttttctaaat
     2281 cagaaattat caagattttg aattctacaa ataatctaaa gcataaactt gttttatctc
     2341 tcttgtattc tgctggtttg cggttaagtg aagttatccg cttaaaagta aaggacattg
     2401 acttatctag gaaaaccatt tttattcgag aaggtaaagg cggcaaagac agaattaccg
     2461 ttctttcgga taagttagtc acagacctat ccatttttat ggaaaatcgt atcggtaaag
     2521 atatactttt tccctccaat caaaaatcag atttaggcaa tgaaaagcca ttatctgcgc
     2581 gcacagttca gaaaattctt tctaccgctc tactaaaatc aggaattcga aagaaaggaa
     2641 cacctcatga tttgcgacat tcctttgcca ctcatcttct agaaactgga accgatatac
     2701 gttttatcca aagacttctc ggtcataaaa atatttcaac cacaaccatc tatacaaaat
     2761 tagcaaatcc taacatcgca ggagttaaat cccccttata gagacaaatt acaactctta
     2821 aaaactcttc aggattttcc gtccgcgaat ttttaccgag tcaatcagta ttgataaaaa
     2881 tttaatatag aattcgaaat gtgataaaga aacaaaacga aatacaaaac aagtcgcctg
     2941 acctactcca caatttcaaa aatccaaatg ataaatcaaa tcaccaatca tctttgttga
     3001 gcaatgatat ctatgctaaa acaaacacat cggaatatat cgcttttcct ctgtgtaaat
     3061 cggtgaatct gaatttacta attttcaatt cctatctatt tgcgtcatat gatttattaa
     3121 acttttgaaa atgaaaatag cctagaagat accaaaagac aatcactact tcatcatctt
     3181 ggaaatagca ttgaaaaaga cctgagaaga aaaatccaat taaaccataa aaaagcaaat
     3241 tgtctgttct agattgattt ggtcttagta gataatagca aatagccgcc gcaagagacg
     3301 cataaaagag tagagctgga attcctgcga tagcagccag atgtagaaag tcattgtggg
     3361 catgtcctct ttgcgttact tcataaaaaa ataaaagctc tgtatacttc tcacttagat
     3421 ttttacgaac tttctctacc tcttttccat aatttcccgg accaattcca agaactggat
     3481 tttcgaagat cattggataa gtagaactcc agataaaagt tcttcctgag tctgtatgtt
     3541 tttcttgtcc taggagtggt tgaattgttt ttgcagtcgt ttcattgaag acaagtaaac
     3601 ttcccaagat tgcaatgagt agaataggaa tcgaaagtag tttgattgta ttttttagag
     3661 aaaaatattt ttgcacaaag attaaatcaa taaatccaat taaaatcgat acacccgctc
     3721 caaataaagc agaacgcgcg ttatttagga gtgctatcga actaaacgca attaaaattc
     3781 ctagatagta taaaactctt tttctatctc gcaccgcaaa taaaaattga aacaccacat
     3841 agggagcaaa aaataaaaag attccagcaa acgttagatg agtattcatc aaaccaatag
     3901 gcaaatgaat tcctattttt aaaatatctc cataatgatg cgtgtatttc cagttgggag
     3961 atggcttaat tagatctgta atgagcctag aaagtcttac ttgtgtaaaa atagaaataa
     4021 agccagttac aattaatacg aataataaga ttgtaaatgc cttctcaact tttcttatct
     4081 cctcttctcc ttttatagat tgaattacta aaaaggcgga gaatagaagt agatctttaa
     4141 attcacttcc cttaaaggat gcggctaatg agaagttgcc actcgtcaat gtatgactta
     4201 aaatcgaaat caaatagaaa ccaaagattc caatcgatag aaaaaaaata ggtgtgaagc
     4261 gaacttcctt gtatcgtttt gtgagtataa agaaaaatac tgttataaat aaaaacaact
     4321 ggcttaaaga aacggaaatg gcacaggcaa cgattcctat ttgcaatgaa attaaaaatg
     4381 ctttttctct attctttaaa ctcaatttta attctcccca tatatgttct cagttccttt
     4441 agacatttgc gaatcagttc acccttttcc tcttctgcaa acgattcaat caaacgtccc
     4501 atatccgaaa tataggtaaa gccatacgcc ccaccatgcc ctttcatttt atgtgcctct
     4561 ttttttaact cttcgaaatt tctattttcc aaaagagact ctaaaagccc caaagactga
     4621 ttcatggaat ctatatactc tggcactaag tcctcaaagt cctttgtgac ttctactatg
     4681 atttcgttgt ttgcatctgt catactgcca actttcgata gactagagaa caataaaatc
     4741 atttttccat ttttacaaaa gactttaaga ctcacctagg taaatttgat tcatttatta
     4801 taaatgaacc aattcagtca tactcaaatg gatcaatcac tgatttgtta gattcagaat
     4861 gattctagtt ggaaactgag ctttctcttc tttttatcct tttgtaatta gaaaagaata
     4921 gatcaaagaa tactatgaag tataaatcta tttagattgg aatttttatt tctaagaaaa
     4981 tcctatcgcc ttctaaactt tgggcaaaag ttccttggac tgattttaac ctattttgaa
     5041 ttcctcttgt tccttctcct gatttttcgc ctactttata atttgtatta gacgttactc
     5101 tccagaacaa atcacttcca tttgaggtaa aaatccattc agaatttgca tatccatatt
     5161 ttaaatcatt gtttgcaatt tcagtagcaa ttgaaaacaa aacttgcttt ttgctatgag
     5221 gaaattcttc aaaaatatca gccaaacctt cttggaattc caatttgcac tttctttcaa
     5281 cagatccaaa ccttctaagt aaataaatag aaagtcccat tgcaaagttt tcttgaatat
     5341 tattaaaatc ttgaatcatc tgcatcttat ttcttacatt ttgattgatc tttctcagta
     5401 gatgaacgtt atattcaata tgcgaatctg gaacagaata atttcttaat tgattctgca
     5461 atattaataa ttcgctaata tctcccccaa tatgatcatg aatatcaaaa aagattctct
     5521 ctttaaattc atgtaaatct tttaactgag cattcacctc tgataattct gcatttttaa
     5581 tcattaaatt tttagaatat tcttcggcta tatcatagat ctcagtatat cgttttactg
     5641 aaataaatgc gatcgaaaga attaagccat taaatccaag agggaaaaaa aatcccctat
     5701 gaggaataaa gccaagcgta tgtaaaaaaa aggaaaataa actaatcagt aaaatggatt
     5761 ttccaatcaa taaaactttt gctaaataat tcccttcact agcagaaaca tatagcctat
     5821 aaagagtata aaagatcatc acagccacaa agatcacaac aggtctttct aaataataaa
     5881 aatgcacaag agaaatctta acagtaaata aaatcagtgt attcagaaga ataaaaaaaa
     5941 gaattactcc tttcccaatt ttataaaact gaaatttatt ttcaaaaaac tctgaaaagt
     6001 aaccaataaa aaaataaata gtcccaagaa ttgtaaatgt attaatatga gtccaataca
     6061 tcggctcatt gtaaattaaa tatttaaagg gagaatgatg aattgtagta attcccgaaa
     6121 gtaaactcaa catactaaaa taaaatacaa tcttgatttc aggtctttgg tataaaaaat
     6181 aaatccctat taaactaaaa caaatatagg ctacaccaga aattcccatc agagcataat
     6241 cttttaaatt ataaaataat aaagagatag tatttcctaa tagcggtatt cctgcaatgc
     6301 ctacatcttt ccaaccactc gcaattcgca aataaagcgt cttacctaga tgttcttttt
     6361 ctaaagataa aatttcgtgt cgattcaatc ctttggtcaa tgaatctgta tctttctcaa
     6421 ttggataatc attaaaaatt attttgccat caatataagc ttcaaatacg tcagtcacat
     6481 tataaaaata aattccaggt cttgccaaat tcagactagg caactgaatt cgaatccaga
     6541 gtatctttgc ctcttccgtt tttttcaaaa agctatcgtt caaaaaagga gaccatttta
     6601 aattggggtc atttagaaaa gtaaacactc cttgttcatt tcttggggaa tctcctattc
     6661 tatactccca tccatttttt aatgggtaaa tttctgtatt ttgaatagga taattcgtat
     6721 catattttaa aaaaccaatc aaaatcgaaa aaatcagaca tatagtaaaa gtataaaaac
     6781 tcttttgata agaaatatta gaactaaaaa aataatggat tcttccctga tctgacaatg
     6841 tctaccgcct atctttctta attcttcatc tagtgctctt gctttttttg tttttttgaa
     6901 aactaaagat cgaatcaaaa tgcgtcaagt tattaaatat ggtgcttcac ttctattgta
     6961 aaaaaaattc tatcacctgt ggaaaattag aacaaaggac atagaaaagc gttggttgaa
     7021 aagtcttctc ttttgatttg ctatttttct aatttctcga tctgtatttt gaataaaaaa
     7081 gaaatctttg caaagaaacg gattctactt ttctaaaatt gacttttcta aaccaatcga
     7141 tttttacctt ttcttttgtt tcaaagtctc cgactctatt tgcttcaaaa ccaagctggg
     7201 agaaagccag agccgatcca atgagtctca tcttccattt tgaaattaaa ctatttttaa
     7261 atgctttcgt tttgccctcg ttgactccaa ttaaacccaa accatttggt caaaataaaa
     7321 cgaaacctag atcccagttt tttccttgaa tctcttaaac tccaattggc ttcctttgaa
     7381 tgaaaatagg cttaattcta tggctcaggc aggcagtgaa cacgaaaact ccaccgtaaa
     7441 aaggtgaaat tttatcgctc taactatatt ttacaattta cttgtatacg atttattagg
     7501 attcaccgat aagtgtatta tgaaaaatat acttatatta attcttttta tctctttttt
     7561 ctcaattcat tcagagccaa attcaagtgg caatactgca aatccttcca ctgatccaaa
     7621 tgcagcgcaa ccaaaagtaa tcgaccaatc taaaatcgtt gtcagtgttc caggtccggg
     7681 tggcggtctt ggcgatacag cttggggtca atccttcatg agtattcggg atcaaattaa
     7741 taagatcaga caaaacccag aagtaaaaga gaaaatagaa attttaaatg aatccaaaga
     7801 taaatctctt ttagtaaaaa gaaataatat aacctattta tacagatttt ataaaaaacc
     7861 tgcaatacta gagaaattca ataaaagcaa agaagatgaa ggaaagagtt cctctctttt
     7921 ttctgttgga gtctatttta gtccagttga aagcctaaaa ctaaagaaac aagtagaaga
     7981 taaatatggc aatccttcta cagagccaca agcgcaaatt ccaaaagata agttttctaa
     8041 accagatgag gaaaacgatt ctgttggaga aaatcaagac gacaagaaaa tcgctggtgc
     8101 ctatacatgg tcttttaaac gagctgatgc tggaaattcc agtgctggat cgaatcctcc
     8161 ccctgcagaa ggagcaaatg caaactccgc tccgcaaagc aacaaagccg agtttattat
     8221 ccaatgggta gaaccttaca acaaaaaagc atttagtaaa cgtatggatt atttcagtgt
     8281 agatagaacc gatttaatca atattgatta caaagactat ttttctgcaa gagaaacgga
     8341 tgtcctttta gatctagtaa atgaaggcgt cttccaagaa aaagaaaaag ataaaccata
     8401 attatagtaa ggaatgagtt ttgaaattat ttttagatac tgctgataca aatgaaattt
     8461 ccaaagttgc tgatttagga ttgttagatg gtgttactac caatcctagc atcattgcct
     8521 caagcaaacg taaattcaaa gaagttataa aagaaatctg tgatattaca aaaggacctg
     8581 tgagtgcaga agttttgtct tctacctact cagaaatgtt aaatgaggca atagatctag
     8641 caaagattgc agataacgtt gtaatcaaaa ttccactcat tccagatggg attaaatgtg
     8701 tttcagaact cacaaaaaga aatatcaaaa ccaatgtaac actttgcttt tcagctcctc
     8761 aagccttatt agctgccaaa gcaggagcga cttatatctc tccttttatt ggaaggttag
     8821 acgatattgc atttaatgga cttgatctaa tctctgagat tagaactatc tatgataact
     8881 accagtttga aactaagata cttgctgcct caattcgtca cccaatccat tttaaagaag
     8941 tagctttaga aggtgcagat gctgttacac ttccctatgc aacgtttcaa atgctcttta
     9001 aacatccgca aacagatttg ggtcttgcta aattcttaga agatgcaaaa aagatagttc
     9061 aataaaggga ttttttttca tttattataa ttctccagat aggatttaag atttgatgct
     9121 taaaaaggga atagcagttg tttttatcta tttccttatc tggatgagtt acacaaatac
     9181 aaaagtattt aatcagaccg attctatttg gagtatctat tccactctaa gcctcgttcg
     9241 agaaaacaat ctagatctag atgaatacaa agatcttttt ccaaaagcat tttatgcaaa
     9301 tctaacacaa gcaaatggac attattacaa ttattatcca tacggagtaa gtctacttgc
     9361 ttatccttat atttggatta ttgataaaag ttattcccta aatcataaag acctaaaaga
     9421 aaaaattctt tctacttcaa ctgtaaattt agagaaaaca attgcagcta gtttacttgc
     9481 actctctgct tgtattttat ttctactcag tctgaaagaa ttaaactcct atctaaatag
     9541 ctttctaact gtttttatat ttgcatttgc gactctcaat ttttctgtct tatcccgagg
     9601 tctttggcaa catactggta gtgtgtttct tcttatgtta gttctatact tgtttcactc
     9661 taaaaaggac actctgatta cactctcagc ctttcctctt tttttctctt atgtagtaag
     9721 accaacaaac cttcttccga ttcttttttt ggggctaatc gccatttacc aattaagatt
     9781 caaatccatt tactttcttg gaatcggaat tgtaattctc tctagctttt tttatacaaa
     9841 ttccgttctc tttggaacca tgagtcaccc ttattatgat tttaaaaaag taggcggaag
     9901 taatacattt ctagaagcaa ttttaggaaa tgcaatcagt cctgctcgtg gaatcctttt
     9961 ttattcgcca gtatttttat ttagttttta tggactctat tctaaaatca aaaaagaaaa
    10021 atttcaaacg ttagactttg ctattatagt tctaattctc actcattgga ttttagtttc
    10081 tagaaattta aactggtggg gtggacattg ttttggatat agacttctct cagatctgat
    10141 tccatttttt atttactatc tgatttattt tataaaagaa atcgattgga aggtaaagtc
    10201 ccctattcta tttctgttta ttctttcgct ttttatttct ttatttataa acttgaaagg
    10261 agctacagag atgaattcct acaattggaa cttaattcca tccaatatag atactgatcc
    10321 caaaaggctc tgggatcttc aagatctacc gtttttgaga taattatttt ttacgaataa
    10381 ttaggattgt catatcatca tcgagttgct ttactcctgc aaaagtgcga actgaatcaa
    10441 atagtttttt agcaaactgc gggaaattgc ttttatcctc attttttatg aactcataaa
    10501 gtctataacc gaaatacttt ctttctttat ttctttgctc aaacaaaccg tctgtgaaac
    10561 aaaaaaggat atcgcctgaa ttcattgtaa aatttctatt attgtattct tctttcggat
    10621 ttgtaggaat atgactcgga acttcaatcc caataaaatg tccactcgtc tcaattactt
    10681 caatatcctt tttatctact ctataaagta aaaaattggg atgaagaccg taatgctcaa
    10741 agtttccctt tgcatctgct tttaataatc ccagcgacgc atagctattg actggaactg
    10801 gataactctc aattctttcc cgaaggactt tattcatcat ggcattcata tctttaggtg
    10861 ttttggctac attgactaag tagttcattt gattgagtat catcatacta aacaaatgcg
    10921 aattgaggtc atgaccagaa gcatccccca ctgcaaacca ataatttcca tcattgtctc
    10981 ttgcatggtt gattatatcc ccacctaaat aggttgccgg ttgcaatttc ccaaaaactt
    11041 catagttctc atcacttaaa ttgaggggaa atacaaaggt ttgcaaattc aagttcacaa
    11101 agtccaaaga atgactttca cttgtatgaa agtaattgag tagatctaaa agtcttttta
    11161 gatattttaa ttctgggaaa tttacaaata aaaagaggat gatgtagata gtattgtaaa
    11221 caatccagaa aagtccatgg ctaatctgaa caaactccaa tggatctaga atataagctg
    11281 ctatagcaaa aagaataaat aaaaccgcat tttttagaat gatactatat ttgtgctgat
    11341 taaaactatt aaaagggttc gccatactaa ctctcagttc tcaggataat aagcatgaga
    11401 ttgaaattct ttgaaagcat atattttaat atactgaaaa aaatttggat ttttatcatc
    11461 aaagggttta gaaacgccat cttttacccc aaaatctttt tctttatatc ccatatcata
    11521 aatcatcgga ataattaaat ttccctcgac tgaataataa taaagtaagg cagattcctg
    11581 acttctctct tccttttttt caatgactcc atttagtttc ttctcagcga atattgactt
    11641 cgtttcaagt aatatccagt tgccatttac cttaaacaca cctttcccga tcacggtatt
    11701 ctcgtgtttt tctctaccca ctaaatccga tgcttcatat actttggtaa aagtaaaatc
    11761 ggatcttaaa tagatcgact ctgaccattg atttttatgc cacgatgaat tgatcgctgc
    11821 tctagaattg gttttctttt tccaaacacc aaaaaaattt cgagtcgagg gaagttcctc
    11881 gtttggtagt gttctatacc atggagtgct gcaattgaat agaaagagta aaaggatacc
    11941 gaatgataaa ctttgtattg cctctttcga ggcataaaaa aactctttca aggtgtttag
    12001 aattggaaat aaataaaatc tccaccacca tctccaaata atccaagcag taaaaataaa
    12061 aagagagaag cgataggaag aattatattc tgaaattttt tccatttcat aacgatttga
    12121 tgattgtatt gtaggaaatt taaaagtaat gcaagaacga tatagagaat tacctcatct
    12181 cttccgccta tatctctacc tttattcatt ccaaaaaaat ctaccgttag tttatacata
    12241 agcgtcgtcg attgagtccc atacgatcca cttctaaaaa atattgcaga catagagaat
    12301 aaaacaaaaa caaacattct tttacaaaaa agtaaaatag ggtttttggg ggttaacgta
    12361 taacctttac taaaaaataa tctttcaatc caaagaatga ctccgaaata agcaccccat
    12421 agaataaaag ctaaatttgc tccatgccag agcccaccca aaaccattgt gataaacata
    12481 ttcacattgc tcatatgaaa cccttttgca ttgccgccta acggtatata caaataatct
    12541 cttagccatg tagaaagtgt aatatgccat cttgaccaaa gctctgcaaa ggttgcagaa
    12601 taaaatggac ctgtaaaatt ttctggaatc tcaaaaccaa gcagaagtgc aagcccacga
    12661 gccatatcag tatacccgct gaagtcagaa taaacttgag cagcaaaacc taagaaggct
    12721 ataaatatgg atacaaaatg atatttatcc ggatccatat aaactggtgc aatcagggat
    12781 cctacgttat ctgcaataac aactttctta aataaaccaa cgatcagtaa aaacaaaccc
    12841 cgcttcattg aatccggtgt aatatcagga ctgtttaatc taggtagaaa gtcagaggtt
    12901 ctcataatcg gacctgctat caactgagga aagaatatga tgaatatagt ataatcaaaa
    12961 aaatcaattc tttctgtcac tcttcccttg tgaatatcta cttgaattgc aatcaattga
    13021 aatgtataaa aactaatcgc aagtggtaaa ataatattta cagaggtgga taagtcctta
    13081 aacatttggg aatgcgataa actataaaaa ctatctagta ggaaatagaa gtatttaaaa
    13141 aaagctagat tgattatgtt taagattaaa ataattttca gtaacttact tgtattttct
    13201 cctttctctt tcagagaaaa catcttatta ctgaagtaat aatttactcc aataacggaa
    13261 aagaaatgga tcgttgcaag aacatcatag taaagataaa acaagaagga tgagatagca
    13321 atgatgtatt ttttatattt gtcttctgta ttccagtaaa ctaaatacgt tacaataaat
    13381 aaaagtagaa atggaattga attaaatagc attaatacgc ccagagatac ataaatttga
    13441 cttccgtgtt tgcttttaga atattttcta aaagattgga actaccggtt tcctctgcca
    13501 tgatttggta aacactccta cttctttgtt ttaggtgaga ataataaatg attcttggtt
    13561 tattattatt aggagtcgca tttctggcat atttttcaga cctcataagt tcaaataaag
    13621 cttcttcaaa ataaccaact ttatccacaa gaccttcttt gaaagcctgt tctgcagtga
    13681 aaattctacc atcacaaact tctctgagcc tttgttcttg aatgttttgt cgatttgttt
    13741 ttacaataga aaagaatctt tcgaatagat tgtttacaat tgattgtaaa atttgttttt
    13801 gttctggctt catttctaag aagggagatc ctatcgcttt atttgcacct gaagtaatcg
    13861 attgatcttt gattccaatt ttatcaagac cctcttttgc attaaaccca gataagataa
    13921 ctcccacaga gccagtgact gccgttggca aagatacaat tcgatcagca gccatcgcaa
    13981 catagtaacc accacttgta gctttatcca taaatagagc gactatcggt attccagtga
    14041 gggttttaaa ctttaataac tctctataga taatatcact tccagtaact gttccaccag
    14101 gagaattaat ttttaatata attcctttaa tatctttatc ttggcttgcc ttaaataaat
    14161 catttatgat ggcagataca gtagattcag acgcccctgc tccaaagata tttccacctg
    14221 aaggattttc agaaatcact ccatcaatcg aaatcactaa aattttatct ttgtcgactc
    14281 cctccattgt atgttcgatc aaatctgctt ctctttcctg accaagcgag ggaagtttaa
    14341 ttgtatttcc tatgatacaa ttactaaaga gtaaaaggaa tcctaaaata aaaccaattc
    14401 taccaatgaa ctcagatctt tttttttcta tcatgttatt tattcctact ggcttcgact
    14461 attttatcta aacgttctac ttgttccatc attttctttt ctagctctaa agcatagtct
    14521 tcatagtttt caattttctc tgggacaaaa aatggctctg aataatcaac ccactccctc
    14581 gcaaaaaact taggaaaacg ctgacggtcc caacttttaa attcatagta ttcatcaaat
    14641 cgagaataaa aagtaatgat tggaagtttt gtgagagatg caatttgaat cactcccgtc
    14701 tgcactttgt atctaggacc tctaggacca tctggagtga aaataggaac tcttccatct
    14761 ttaattgctt ttaataattg tcgaagtgca gttgagccgc ccttactagt agagcctctc
    14821 acggattcca ttttgaatct aagaaaggtt tgatagataa attctccatc tttagacata
    14881 gacacaagtg gagtcaaaat gatatgcctg tctctttgta aataataggc aacgtgtcta
    14941 gtcaaagaaa gaatttgatt gtgccagcct gcaaacacaa acccgttttt ttgttttaaa
    15001 tatttttctg ttgcttctgg aacaaagtat tcttgctttc taataaaaaa aaaccaaaaa
    15061 gataaaatga attttgctat ataactaact attatgtatt tcatggaact aatacaaata
    15121 tcaaaaacta gttttaaaaa tctatcttat tttagataga atcaaaaaat ggatggctct
    15181 tgatatcggt gtaatactct tcattaaaca gagaggttaa ctcctcagta acattataat
    15241 aattcataat tgattttaag atattggaat cctcttctga gattgtaatt ccaagcagtg
    15301 ataaagaaag aatttcagcc attctaagaa tccgatcaat gtcttctact ctagatgttt
    15361 cctcttgcgt aaaaccctga ttagccacaa ctttttttaa actatttggt aatttccaca
    15421 tctgaaatac tttgatccca atttgaatgt gatcgatatt taactcttct ttctctaatt
    15481 gaaccaatgg ctttgatcca tattcaaata attttaaaat atccaagtat tttaatttta
    15541 gattttgtgc aagaattgac atacccactt tacgaaggat agagctaata acgatttgct
    15601 ctcttacatt ttttaaattc aatggacctg ttaaatcaat tccaatcaaa ccagttaata
    15661 taggaatttc atttaggtgt tttctaaata aaggttgtgg aaggttcttc gtaaaatttt
    15721 ttctaaattg taaaatcaca atgtttcgaa tggttccaag cccaatcaga gtgattgcat
    15781 tttgtagtgt gtctacactt ccagatcttc catagtaagc agaattggca actcgcaaaa
    15841 tatctgcact taacgctttg tctggtgcaa tgatcatttc caattccttg cttcctccgc
    15901 ttttctttga atcaaatgta atcactcgat tcattacatc tcgcaaaggt ggaatttgta
    15961 tttcgttgag gttaataccc atttgcttat aacctacgac catcttattt gttaaatctc
    16021 tcctacctaa aagaaaggtt aacttttcat agatggcaga ttttgtaata ggttttaaaa
    16081 taaaggcgtt tacctttagc ctcactgctt cttctacaat ttccttttct gaaaaagccg
    16141 taattagaat gatgataagc ttaggaaaaa atgttcttat ctcttctata acttgccctc
    16201 catttttttc aggcatagaa taatcaacac agagtatatc aacttgtcca ttcaactctt
    16261 tgattttatg aatcgtctct tccccacctt ctgcttcgcc aatcacttta aaatctgcag
    16321 attttaaaaa ttgctgaatc aactttctct ctgatattga atcatccgaa ataaaaacat
    16381 tatacggaat catcttacgc ggattaatcc ctaaaggtat ttcacttgcc ataaacaaaa
    16441 acaagctaat ttaatatggt ttagaatcaa ctgaaaatac aacgggatga ctcaggattt
    16501 ttttttgcaa gattcaatgt tttctctcaa gacaaactct agtgttggaa attttaaaga
    16561 ggaatgagag cagattaaaa caatccttaa gaattataaa aaatatatcg atgtattaat
    16621 acgtagtgaa aagtctactt tacttttttg tatttagata actacatggt tacaatagat
    16681 at
//
LOCUS       DAOQOS010000118        16229 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_1809_length_16229_cov_5.619575, whole genome shotgun sequence.
ACCESSION   DAOQOS010000118 DAOQOS010000000
VERSION     DAOQOS010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 16229)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16229)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16229
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1809_length_16229_cov_5.619575"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            351..1421
                     /locus_tag="PLF66_20155"
     CDS             351..1421
                     /locus_tag="PLF66_20155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66414.1"
                     /translation="MKPSAKKAIQKNTKKTVPKRTAKKPTPKKEIIQTDIKPLPIKKF
                     PGLGINVVLKHTDESSMKTYIHYLKELRVEWVRLEFNYYDALPDSVMDFFVEELRKAD
                     IKILGLLTGLVPGNFINCMIPSLRFKSPIDTLEDYKEFCEKLGTRYIKQITSWEIWNE
                     PNTLRFWIRNPDPIEYVQLAASTAPILKRINSKNKIVFGGLMGDDLRVVAPFQKINFI
                     QECIDGGIDPYIDVYNFHPYDPICYFAKKPANVYFPTIAGVIDSFLHKYRAVRKPFII
                     SEIGVCPLWLKITQTEIGQLYKEIYLHSISKRVDCYFWVLTDFKGPEYSRWNPETAFG
                     LVTWDLKEKEMYKSFVTEMKKL"
     gene            complement(1624..2235)
                     /locus_tag="PLF66_20160"
     CDS             complement(1624..2235)
                     /locus_tag="PLF66_20160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66415.1"
                     /translation="MQLLEWKIFQFVEIIFISGVILNLLSYLIRFIYLYRKKQMKTFA
                     RTVKEGFSKTILVKNAFSISMILTSLSVFWYMLHIGKDLKTLTIAQALKENIYVTGYI
                     LLAATLLTIYSFLVAYYNILRLRELSPTLNFLSYWNFTDQVFHLIGGGVAFKPITDFS
                     ISFRKYLIQSRMSELIVSTLLLILFEYIYKILMVVALLYLLKV"
     gene            2415..3596
                     /locus_tag="PLF66_20165"
     CDS             2415..3596
                     /locus_tag="PLF66_20165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008596427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HMZ66416.1"
                     /translation="MNTKTILILLLGTMTAIGPFSIDMYLPGLQSISKDLNTPIENVQ
                     LTLSSFFFGFSFGQILYGPIIDRYGRKLPLLVGLALYIITSIGCVLANNVWSLFVLRF
                     LQSLGACSGMVISRAIVRDAFTPHEGAKVFSQIILVMGVAPIIAPTVGGLILSVASWR
                     YIFVSLSGIGILTFIGVYFYLTGTKEADQTVSLSPTKVLKEYWNVLLLPRFYKYAIAS
                     SFAASVMFAYIAGSPFVFMSLFQLTEQQYGWIFAFNAAGLILSSQLNRVLLQKYESEK
                     IITIVAYLYLPLGILLLASAYFGLGFIPMLTLIFLLVSGFGFIVPNASALCLAPFTHN
                     AGSASALIGGLQMIFAVLASSLVSLFHNGTALPMASVMAMGGFMTIVSLFVLKIPHAN
                     S"
     gene            complement(3591..4118)
                     /locus_tag="PLF66_20170"
     CDS             complement(3591..4118)
                     /locus_tag="PLF66_20170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014801900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HMZ66417.1"
                     /translation="MKISVYIATSLDGFIARMNGDIEWLTSAGIPGDTEDYGYYSFTE
                     SIDAIVMGRNTLEKVLSFPEWPYSKKVFILSNTLKSLPETAKDKAELFSGDLKTLKEK
                     LELNSFKRIYIDGGKTIQSFLREGLVTDLTITRIPVLIGSGLPLFSSLSKDIKLKHIE
                     TKSYPSGFVQSKYEL"
     gene            4213..4833
                     /locus_tag="PLF66_20175"
     CDS             4213..4833
                     /locus_tag="PLF66_20175"
                     /inference="COORDINATES: protein motif:HMM:NF014906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HMZ66418.1"
                     /translation="MKKRSIGRPEKNILNQDKIITAALDLIDKNGVSNLSMRELAKNL
                     KVDPMAIYHYLPNKESLLHEIVIYIFQSLDTSLQYPNNWKKQVKFLLNNYRNLVKKHP
                     NLIYFILTEPAYSTKPSLELNEKLFYFVSLSNLSKQKSILLGNLLVDYLHGFSLAENT
                     MTGEIEPFSIEENGDYPHFAEAINFNLNKKDSLFETTIDFILSSFE"
     gene            5233..5745
                     /locus_tag="PLF66_20180"
     CDS             5233..5745
                     /locus_tag="PLF66_20180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66419.1"
                     /translation="MKKIFLILTFLVTLSFCTSVSVAEERETPKIDVALNRLLQSHLK
                     FISEWQNSSSELDEFDKIFFTDFNFLNAGNTKNFFQNIQHNLESGKVYPGLMQVSYSS
                     FTSINGKVTGVNYEYKSDGKNIVLTKGTYNNGEMLRAVYNYNIDGKLLNTTEVKGNKL
                     LNKKSYRLEI"
     gene            5795..6217
                     /locus_tag="PLF66_20185"
     CDS             5795..6217
                     /locus_tag="PLF66_20185"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004788815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HMZ66420.1"
                     /translation="MEKTKRIVLVAHDNKKKDLLDWVSFNKGTLAKHFLSATGTTGKI
                     ISEHTGLPVNRFISGPLGGDQQIGAKIVEGGIDIMIFFWDPLSAQPHDPDVKALLRVA
                     VLYNIPMACNRSTADFLISSHLLDEEYENLSHESTKPN"
     gene            6238..6801
                     /locus_tag="PLF66_20190"
     CDS             6238..6801
                     /locus_tag="PLF66_20190"
                     /inference="COORDINATES: protein motif:HMM:NF013052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
                     /protein_id="HMZ66421.1"
                     /translation="MKLFLSILLIINSCAVTSVKSIEDNEYVVIKPSHIPGAGMGLFA
                     AKDIPKDTMVTHYEGKKVTRKEYNAIHEKSEHWYMFTMPECANEPNYPYLDGNRDHYG
                     SKVNFAPSKINGKPTKLQNVHFLKHCEEPYIRLYASRDIKKGEELYVSYGGIYNYHFM
                     EFPDVQKFFLEKSGIKLKPNEKFTFED"
     gene            complement(6995..8677)
                     /locus_tag="PLF66_20195"
     CDS             complement(6995..8677)
                     /locus_tag="PLF66_20195"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing methyl-accepting
                     chemotaxis protein"
                     /protein_id="HMZ66422.1"
                     /translation="MKIKNLILPASFFVAFLNLFFSIVTILISYKSYQNSEKILNAEI
                     PLFNSAWAILLEDTKITDNAIFFSYSGENQYKDNYDQHVKALEKELEYSMKNSGTQEK
                     KIFENVSTVNDVLINLETKMFTLGGSGKLGEAQAVLKADDYHKNKKIYSDAVMKFFQS
                     QQDRLSETLRTQISNSRLLFYSAFILSLLGLSASLFITLWLSRGVIKPLEKAADYSGS
                     ISKGDLTLEVESKGKGEFADLFRSIGGLVNKFHQVVEQIFLASEDVKSSSTNMKIAVV
                     MMRDDLEKQTAGSEAIAKKIFEISNASNSINIMTSEQSNKIEQMLSKLKEQSSLVHNL
                     GEKTKITTRDTGVISNELDQGKTSLNSLNERMQEIYNTTSQITEVVSIINTIADQTNL
                     LALNASIEAARAGNEGKGFAVVAAEVSRLADQTAGSTKSIKDIVRKNSALVKAGMDGL
                     AELTSMFDGILERVKGINVKTNDVKGLIDEYSQLTSHLEVTIQNVKAISAEIFKSTDE
                     HLKAISTISETATSISKVTLSNFTRLDALSDDSKFLESVSDNLKSQVSFFKL"
     gene            8889..10193
                     /locus_tag="PLF66_20200"
     CDS             8889..10193
                     /locus_tag="PLF66_20200"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020778239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HMZ66423.1"
                     /translation="MKVCVIGSGYVGLVAGACFAEYGNDIVCVDIDEKKINNLKNGII
                     PIYEPGLSEMVSHNVRRKRLEFTTSLKYGVEKSDIIFIAVGTPTGAEGEADLSMVLSV
                     AKEIGKAMNGYKIIVDKSTVPVGTAEKVKAVVEKETTHPFDIVSNPEFLKEGVALEDF
                     MRPERVVIGADSDQAGQTMKELYAPFVLNGNPILLMSTKSAEITKYACNAFLATKISF
                     ANEIANLCDKVGANYEDVRNGMGTDSRIGKKFLYAGIGYGGSCFPKDVRALIKTAKDV
                     GTPIRIIEVVEDVNEKQKVKILAKIEAHFKGESLRGKTFAVWGLAFKPDTDDMREAPS
                     IPIITELHRLGVNLQVYDPEAEETSKYYFNGKVKYATGAYEALEGAEALLLLTEWREF
                     REPDFGRVKNLLTKHVIFDGRNLYKKPQMKRLGFTHYGIGMN"
     gene            complement(10200..11324)
                     /locus_tag="PLF66_20205"
     CDS             complement(10200..11324)
                     /locus_tag="PLF66_20205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HMZ66424.1"
                     /translation="MKKTIFILLTMMLVALLSHCNYEIEDRSNTAILSYIDRVLRSTS
                     STPATNASDTAPPTVGAISILNVSSSSITIGWTAATDDFTVQSNIQYLPYFSLSSNLN
                     TVSNIETNGMPVGTFTSNLTQVSVINLVPQTNYYFSVIARDAQGNKAAYLPIAGLTTQ
                     DTMPPIPGNSGILSANTVTSTSITVGWVSATDNSTPQSNLQYKVVYSLSNNITTISMA
                     ETNGTIAQNWISNLSSFSINGLAMNMPYYITVLAMDTYGNKAIYSVLNVSTLTCSPGP
                     GILSAGYIWYIGCPNVNCNTTCIGRGGVSPGYTFIGTNTGNCQMILDVLGLGSGAVSP
                     QPCAQGAGCYINPGRWLCTSPAFDVNFSSPSDIKACSCAL"
     gene            11619..12113
                     /locus_tag="PLF66_20210"
     CDS             11619..12113
                     /locus_tag="PLF66_20210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66425.1"
                     /translation="MNQFISLTLFAFLITSGISAVERIQQENSKVIDEFAKTEEFIKN
                     RKISKEYRKKTLEKNLTNAIKYTMHKKFPDYQARIKDLKPDAVKYEQEKGTFNYYLNY
                     KDYYIFYNFAVDPEIYLQLPADEKMYIKLADQDKETASNPPPAPAGNAGNDKGPNKPI
                     GTNP"
     gene            12119..12982
                     /locus_tag="PLF66_20215"
     CDS             12119..12982
                     /locus_tag="PLF66_20215"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HMZ66426.1"
                     /translation="MFDKNSKEFHLAISSVLEAGKIVRSIYDTNFEVSWKAKDDPLTE
                     ADLRANQEIIRQIQLTFPEDAILSEEISDSEKRLSKERVWIIDPIDGTREFVKKNPEF
                     SVSLGLSVSGKAFIGSILNPITRELFVGIVGQGIAYLVLKDDFSLDLNQLNLTKHDFK
                     PMDSKPFTYVSRSEFFKYKLFDQNPYWKENYDLKPVGSIAYKLALAAANLGDLALSLK
                     PKSEWDICGGVALIEASGGIAVDLKNQKAFPFNEKSPLQHGILAGNPFLVEKILKEKG
                     NFFRDSLVNWE"
     gene            13213..13356
                     /locus_tag="PLF66_20220"
     CDS             13213..13356
                     /locus_tag="PLF66_20220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66427.1"
                     /translation="MGKIKPQGSILLIEKPISLLSSEPEPDIAIVKDPFTTLNLKTQK
                     QPF"
     gene            complement(13765..14703)
                     /locus_tag="PLF66_20225"
     CDS             complement(13765..14703)
                     /locus_tag="PLF66_20225"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HMZ66428.1"
                     /translation="MLIQFWILIVFAITIIFTSVAFASPFDSFENELKEYIDSESGQY
                     YEGEDSQLKELFSPDEETAEDSNKSVGTYSRISSNYKLPSHIQIARVISATVIKPTTT
                     VVGGYKVQAKDTLSLIARRYKMKVEQIKKLNGLKGDLIRIGQVLRINSSKPTTSQMAG
                     GTIYKMKVFALPVLTGKVTSRFGYRRDPFNPSVRNYHSGLDLSAPVGTPVIASAEGVV
                     EFKGRNGGYGNTVIIRHKGGYKTVYAHCSTTVVEVGDFVKMGKVIGSVGRTGTATGAH
                     LHFEVIYKGKLIDPEKALRKIEIVTTKPGVTLANKS"
     gene            complement(14751..15146)
                     /locus_tag="PLF66_20230"
     CDS             complement(14751..15146)
                     /locus_tag="PLF66_20230"
                     /inference="COORDINATES: protein motif:HMM:TIGR04410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system-associated lipoprotein"
                     /protein_id="HMZ66429.1"
                     /translation="MFRSIFIFLSIFFILACNKRLIKKDDLTLINEHYSQNTYYVKEN
                     LVLDNKDIIKKDTSVKIWIESTASILKVKCYNSNEDRESAVGRMVTYIINEDFKNKKF
                     TVEYLDQLIAEKLVYYDAKDVSKDKKAKK"
     gene            complement(15170..>16229)
                     /locus_tag="PLF66_20235"
     CDS             complement(15170..>16229)
                     /locus_tag="PLF66_20235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004491673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66430.1"
                     /translation="DLPVFDCANKCGRYGKRFLSYQSHIRTMAAAQPFISGAISKTIN
                     LPEEATLEDVKDAYLISWKLMVKANALYRDGSKLSQPLNSVSEMLHFAEEEVSEEDNE
                     KPPIIKTTEKIVLKYISQRRKLPSRRAGYTQKATVGSHKVYLRTGEYEDGQLGEIFID
                     MHKEGAAFRSLMNAFAIAVSLGLQHGVPLEEFVEAFTFFKFEPNGMVNGNQHIKMSTS
                     VIDYIFRELAITYLGRYDLGQVVPDEELRGDEVGKPGRLDSEMNESLSEISENKPSSN
                     GMKPMPISLSDVISRNDSALLKKEASIAVLDAIGQREKAKIQGYTGDSCVECGSFQMV
                     RNGSCLKCNSCGSTTGCS"
BASE COUNT         5398 a         2944 c         2738 g         5149 t
ORIGIN      
        1 ttttagtctc cttttggaag cctaaaatcg tgtccttttt ttatgtaggg gttcaaggat
       61 atttattcac ttcgtgagtc ttcgtgcatt cccgcccatc gcttgacact caccatagcc
      121 aagctatgtg gttaaaaatc cttaagttga cagcattacc tgtcggcaac acttcgacag
      181 acgctcagtg catcacttgg gctaccgtcc aaacccgttt ttgggcttaa aagttattgt
      241 taagctttct gattcctcaa ttttttccaa gctgagtagt tactctcaaa gaaagaattg
      301 gaggaaaaaa aattatggaa gaaaatgagt gcaattaaaa agtaatcgtt atgaagccga
      361 gtgcaaagaa agcaattcag aaaaatacca aaaaaacagt tcctaaaaga actgctaaaa
      421 aaccgactcc taaaaaagaa atcattcaaa cagatataaa gccattacct ataaaaaaat
      481 ttcctggtct tggaatcaat gttgtattaa aacacacaga tgaaagttcg atgaaaacct
      541 atatccatta tctaaaagaa ttaagagtgg aatgggttcg tttagaattc aattactacg
      601 atgcattgcc tgattcggtg atggattttt ttgtagaaga gttaagaaaa gcggatataa
      661 aaattttagg attactcact ggacttgttc cggggaattt tataaattgt atgattccct
      721 ctcttcgctt taaatctcct atcgatacat tagaagacta caaggaattt tgtgaaaaat
      781 tagggactcg atatataaaa caaatcactt cttgggaaat ttggaatgaa ccaaatacac
      841 ttcgcttttg gatcagaaat ccagatccaa ttgaatacgt gcaattggct gcatccactg
      901 cccctatttt aaaaagaatc aattcaaaaa ataaaatagt atttggcggt ctcatgggag
      961 atgatctgcg agtagttgct ccatttcaaa agatcaactt tattcaggaa tgcatagatg
     1021 gagggattga cccatacatt gatgtctata actttcatcc ctatgatcca atttgttact
     1081 ttgcaaagaa gccggctaac gtctattttc caaccattgc tggagtgata gattcttttc
     1141 ttcataagta tagagctgtt cgaaaaccat ttatcatcag tgaaattgga gtttgcccgc
     1201 tctggttgaa aatcactcaa acagaaattg gacaactcta caaagaaatc tatctccatt
     1261 ccatcagcaa aagagttgat tgctatttct gggttttaac cgactttaaa ggtcctgaat
     1321 attctagatg gaatcctgaa actgcctttg gtttagtcac ttgggattta aaagaaaaag
     1381 aaatgtataa atccttcgtc actgaaatga agaagttgta gtgaaaataa acagagatat
     1441 aattgaaaga aaaaatgaat gaacttcagt tcggtttaac aaaaacttca taagataaac
     1501 accgatgaca ctgataatgt agagaaattt aaaatcacct aggttatcgc ttgtgaaaaa
     1561 ttcttatatc gaatttaggt taaacaaaaa gatcgattct aatttcattc tattctctct
     1621 ttatcaaact tttaaaagat aaagcagtgc aaccaccatt aggattttat atatatactc
     1681 aaataaaatc agtaaaagag tagaaacgat aagctctgac attctagatt ggatgagata
     1741 tttacgaaag gaaatgctaa aatcagtgat tggtttaaag gcgactccgc ccccaattaa
     1801 atgaaatact tgatcagtaa aattccaata cgataaaaaa ttcagcgtag gagaaagttc
     1861 tcgcaagcgt agtatattat aataggcaac taaaaagctg tagatagtaa gaagagttgc
     1921 ggctaataag atgtaaccgg tgacatagat attttctttt aatgcctgcg caatggtgag
     1981 agttttcaaa tcttttccaa tatgaagcat ataccaaaag accgaaaggg atgtaagaat
     2041 cattgaaatc gaaaatgcat tttttactaa aatcgttttg gaaaatcctt cttttacagt
     2101 tcgagcaaat gttttcattt gtttttttcg atagagataa ataaaacgaa tcagatatga
     2161 gagtaaattt aatatcaccc cactgataaa aataatttca acaaactgaa agatcttcca
     2221 ttctaaaagt tgcaagtgat taccctcaaa cttacgcaaa tcaagctcgt ctttttacgt
     2281 atttctattt atctaaatca tgtgttataa caagaaccaa tgtcaagcga acaaatcatt
     2341 caattcaaaa agaaatcaaa atagaataat aaaaatgaaa tcgactttcg aattctattc
     2401 tttccactga caatgtgaat acaaaaacaa tattaatcct acttctagga actatgactg
     2461 ccattggccc tttttcgatc gatatgtatc ttccgggact tcaatccatt tcgaaagatt
     2521 taaatactcc cattgagaat gtgcagctaa cactgagtag tttcttcttc ggattttcct
     2581 ttggacaaat tctatatggt ccaatcatag atcgatatgg aagaaaactt ccacttctag
     2641 ttggcttagc tttatatatt attacatcga ttggttgtgt tttagcaaat aacgtctggt
     2701 ctttatttgt tctaagattt ctacaatctc ttggtgcatg ttctggaatg gtaatctcta
     2761 gagcgattgt tcgagatgcc tttactcccc atgaaggtgc aaaagtattt tctcaaatca
     2821 ttttggtaat gggcgttgct cctatcatag ctcctacagt tggtggactc attctttccg
     2881 ttgcaagttg gaggtatatc tttgttagcc tctcaggaat aggaattctt acattcattg
     2941 gagtttattt ttatcttaca ggaacaaaag aagcagacca aactgtatca ctaagtccta
     3001 caaaagtgtt aaaagaatat tggaatgttc tactccttcc ccgtttttat aaatatgcaa
     3061 ttgcttctag ctttgcagcc tctgtgatgt ttgcttatat agctggatcc ccttttgttt
     3121 ttatgagttt atttcaacta acagaacagc aatatggttg gatatttgca ttcaatgcag
     3181 ctggactcat tctatcaagc caattgaaca gagtgttact gcaaaaatat gaatccgaaa
     3241 aaattatcac cattgtggca tatctttatt tgccgctagg gatcttactt cttgcctccg
     3301 cctattttgg acttggtttt attcccatgc ttacactgat tttcttactt gttagcggat
     3361 ttggttttat tgttcccaat gcctctgcgc tctgccttgc tccatttaca cacaatgctg
     3421 gaagtgcttc tgccctaatc ggaggactcc aaatgatttt tgcagttctt gctagttctc
     3481 ttgtgagttt atttcacaat ggcacagcac ttccaatggc ctctgtcatg gctatgggag
     3541 gatttatgac gatagtatct ttatttgtat taaagattcc tcatgccaat tcatagttca
     3601 tatttagatt gaacaaaccc agaaggataa gattttgttt caatgtgttt taatttgata
     3661 tctttggaaa gagaggaaaa caatggtaaa ccgctaccta tcagaaccgg aatacgagtt
     3721 attgtcaaat ctgtgacaag accttctctt aaaaaagatt gaattgtctt tcccccatca
     3781 atatagatcc ttttaaaaga attcaattct aatttttcct ttaaagtttt cagatctccc
     3841 gaaaataatt cagctttgtc ttttgcagtt tcgggaagag atttcagagt attagaaaga
     3901 ataaaaactt ttttagaata aggccattca ggaaaagaaa gaactttttc taatgtattt
     3961 cttcccataa cgattgcatc aatactttct gtaaaggaat agtatccata atcctctgta
     4021 tcaccgggaa ttccagccga tgtaagccat tcaatatccc cattcattct tgcgatgaac
     4081 ccatctaaac ttgttgctat ataaacagaa attttcattc gatttctcct aaaaagaatt
     4141 tacaaaaaca attaattcta cactgtagaa tttgtaaagc attttaaact tcagaaagta
     4201 aaaggttata ttatgaaaaa aagatctatt ggcagaccag aaaaaaatat tctaaatcaa
     4261 gataaaatta tcacagcagc tctcgatctc atagataaaa atggagtatc taatttaagt
     4321 atgagagagc tagcaaagaa tctaaaagta gatccaatgg ctatttatca ttaccttccc
     4381 aataaagaat cattgcttca tgaaattgtg atttatatat ttcaatcttt ggacacatct
     4441 ttgcaatacc caaacaattg gaaaaagcag gtaaaatttc ttctcaataa ttatagaaac
     4501 ttagtaaaga aacatccgaa tcttatttac ttcattttga ctgagcctgc ttatagcacg
     4561 aaaccatctc tagaattaaa tgaaaaacta ttctattttg taagcttaag caatctctca
     4621 aaacaaaaat caattctctt aggaaaccta ctagtggatt atttacatgg cttttctttg
     4681 gcagaaaata caatgacagg agaaatagaa ccattctcga tagaggaaaa tggcgattat
     4741 cctcactttg cagaagctat caattttaat ttaaataaaa aggattctct atttgaaact
     4801 acaattgatt ttatattatc ttcttttgaa tgataccgtt tttattttta gatagaatca
     4861 atctagatta catagtctga tgaaatattt tctatgggaa gcaagaaaaa ccaagtctta
     4921 taaataaaga tagcatattt caaaacccga tattgcgaca atgaatgaat ttgagtattc
     4981 gatgattatt ttaaaaatac aagtgaacgt aaataaaatc aaaagcactt tgctacttct
     5041 cttcttgctc tcttcaagcc cactttattt tttaatgaca ataaaagcaa acgacaaagg
     5101 attacttgac tcccctagac tagaacgaaa gaagaaattt attttaaaaa ttacaaatga
     5161 ttttttattt gaaaaaaact tttttaaaaa atattttgtc aaaaaagtac agctatggag
     5221 gaaaaattta gtatgaaaaa aatattttta attctcacat ttttagtaac gctttctttt
     5281 tgcacttccg tttccgttgc tgaagaaaga gaaaccccta aaattgacgt tgcgctaaat
     5341 agacttcttc aatctcattt aaaatttatt tcagagtggc aaaactcctc ttccgagtta
     5401 gatgagtttg ataaaatctt tttcactgac tttaattttt taaatgcagg aaacactaaa
     5461 aacttttttc aaaacatcca acacaatcta gagtcaggta aggtctatcc tgggcttatg
     5521 caagtttcct attctagttt tacatccatc aatggaaagg taacaggtgt aaactatgaa
     5581 tacaaatcag acgggaagaa tatagttcta acaaaaggca cgtacaacaa cggtgaaatg
     5641 cttcgtgcgg tatacaatta caacatcgat ggaaaattat taaatactac agaagtaaaa
     5701 ggaaataaac ttctgaacaa aaaatcctac agactcgaaa tttaaagtcc agcaaaacag
     5761 ttgacattcc atagaatcag tcaagtctga ttctatggaa aaaacaaagc gcatcgtact
     5821 cgtcgcgcat gataataaaa agaaagatct actagattgg gtgagcttta ataaaggcac
     5881 actcgccaaa catttcttat ctgctacagg aacgactggg aaaattatct ctgaacatac
     5941 aggtcttccc gtaaaccgat ttatttctgg acctctcggt ggagatcaac aaatcggtgc
     6001 aaaaattgta gaaggtggga ttgatattat gatctttttt tgggatccgt tatctgcaca
     6061 gcctcacgat cctgatgtca aagcactttt acgtgtcgca gttttgtata atattccaat
     6121 ggcatgcaat cgttctactg ctgatttttt aatttcctca catcttctag atgaggaata
     6181 cgaaaatctt tcacacgaaa gcacaaagcc aaattagtct gaagtaagga aatccaaatg
     6241 aaactttttc tatccatttt attaattatc aattcttgtg ctgtcacaag tgtaaagagc
     6301 attgaagaca acgaatatgt tgtaatcaaa ccatctcaca taccaggagc gggtatgggt
     6361 ttgttcgcgg caaaagatat tcctaaagat acaatggtca ctcattatga aggcaagaaa
     6421 gtgactcgta aggagtataa cgcaattcat gagaaaagcg aacattggta tatgtttaca
     6481 atgccagaat gcgcaaacga gccaaattat ccatatctag atggaaacag agaccattat
     6541 ggatctaaag taaattttgc tccatccaag ataaatggaa aaccaaccaa actccaaaat
     6601 gttcattttc taaaacactg tgaagagcca tatattcgtc tctatgcgtc acgcgatatc
     6661 aaaaagggtg aagagctata tgttagctat ggcggaattt acaattacca ctttatggaa
     6721 tttccagatg ttcaaaaatt ctttttagaa aaatcaggaa tcaaattaaa accaaacgag
     6781 aaatttacgt ttgaagattg atttttttat cgaatactag atattttcgt tggctatact
     6841 taatttttat ataaattagc gagcgacttt tatttaattt tgtaggcaag aaatcaattg
     6901 ctcgtctaca aaattaaatc caaaacaacc acgtatccca aacaacaaaa ctacaatcaa
     6961 tagaaacaat cgcctgtttt gtgctctgcg agaattataa cttaaagaaa ctaacctgac
     7021 tctttaaatt atccgaaaca gattctaaga attttgaatc atctgagagt gcatctagac
     7081 gagtaaaatt cgaaagtgtt actttgctaa tgcttgttgc tgtttccgat atggttgaaa
     7141 ttgcttttaa atgctcatca gtagatttga atatctcagc agaaattgcc ttcacatttt
     7201 gaattgttac ctctaagtgc gaagttaatt ggctgtattc atcaatcaaa cctttcacat
     7261 cattggtttt gacattaatt cctttcactc tttctagaat tccatcaaac atagacgtta
     7321 gttctgcaag tccatccatt cccgctttca caagtgcaga attttttcga acaatatctt
     7381 taatcgattt agtgcttccc gctgtttgat ctgcaagtct tgatacttcc gctgcaacta
     7441 cggcaaaacc ttttccttca ttcccagctc tcgctgcttc aatcgatgcg ttaagagcaa
     7501 gaaggtttgt ttgatctgca attgtattga tgatagaaac aacttctgtg atttgactag
     7561 ttgtattata aatttcttgc attctttcat tgagtgaatt gagagaagtt tttccctgat
     7621 ccagttcatt tgaaattaca cctgtatcgc gagttgtaat tttggtcttt tccccaagat
     7681 tatggactaa ggaagattgc tcttttaatt ttgagagcat ttgttctatt ttattacttt
     7741 gctccgacgt cattatatta atgctattac tcgcattgga aatctcaaaa attttctttg
     7801 caattgcctc tgatcctgca gtttgttttt ctagatcatc tctcatcata acgaccgcaa
     7861 tcttcatatt ggttgatgag gacttaacgt cttcagatgc taagaagatt tgttctacaa
     7921 cctgatggaa tttatttaca agtccaccga tagatctaaa aagatcagca aattctcctt
     7981 tcccttttga ttccacttct aaagtcaaat ctcctttcga gatggaacct gaataatcgg
     8041 ctgctttttc taatggtttg atcaccccac gcgaaagcca aagagtgata aaaagagagg
     8101 cactaagtcc taataaagat aatatgaatg cagagtaaaa aagtaatcta gaattagaaa
     8161 tctgagttcg caatgtctca gaaagacgat cctgttggct ctgaaaaaat ttcattactg
     8221 catccgaata aattttctta tttttatgat aatcatctgc ctttaatact gcctgtgctt
     8281 cgcctaactt tccggatcca ccaagagtaa acattttagt ttctagatta attaatacat
     8341 cattcacagt agagacgttt tcgaagatct tcttctcttg ggttccggaa tttttcatgc
     8401 tatattctaa ttctttttct agggctttta catgctgatc ataattgtct ttgtattgat
     8461 tttcccctga ataagaaaaa aagatcgcat tgtctgtaat ctttgtatct tccaatagga
     8521 ttgcccaagc actgttaaat aggggaatct ctgcattcaa aatcttctct gaattctgat
     8581 acgatttata actaatcaga atagtcacaa tcgaaaaaaa taaatttaaa aaagctacaa
     8641 aaaaggatgc gggaaggatt aagtttttaa ttttcatact ctaaccaata gttactctac
     8701 tatttaaatc ggtcattttc cttgcaatct tattcaataa agttggaata tattttttct
     8761 ctacttagaa cttcccatac gaattcaatc ctaccaaatt gtcttgatta aaatccctca
     8821 tattagcact ttgtgaatgg aagtaggcag atagcaatta ctccgaaatt aaagtaaggg
     8881 aatcatttat gaaagtttgt gttattggta gtggatatgt tggactcgta gcaggtgctt
     8941 gttttgcaga atacggaaat gatattgttt gcgttgatat tgacgagaag aagatcaata
     9001 atttaaaaaa cgggattatc ccaatttatg agccaggtct ttctgaaatg gtttctcaca
     9061 atgttcgcag aaagagactc gaatttacta cttctcttaa atacggagta gaaaagtcag
     9121 atattatttt tattgctgtc ggaactccaa caggagcaga aggcgaggca gatctttcaa
     9181 tggtcctttc tgttgcaaaa gaaattggaa aagcaatgaa tggatataaa atcatcgtag
     9241 ataagtccac tgttcctgtc ggaactgctg aaaaggtgaa ggcggttgta gaaaaagaaa
     9301 caacacatcc atttgatatt gtttcaaatc ccgaatttct aaaagaaggc gttgcattag
     9361 aagacttcat gcgaccagaa agagttgtca tcggagctga ttcggaccaa gcaggacaaa
     9421 ctatgaaaga attgtatgca ccttttgtat tgaatggaaa tcctatctta cttatgagta
     9481 caaaatcggc agaaatcaca aaatacgcct gcaatgcctt ccttgccacc aagatatcct
     9541 ttgccaatga aattgcaaac ctttgcgata aagtaggagc aaactacgaa gatgtccgaa
     9601 atggaatggg aactgactct agaatcggga agaagttttt atatgctggg attgggtatg
     9661 gcggatcttg ctttccaaaa gacgttcgtg cactcattaa aacagcaaaa gatgtcggaa
     9721 ctccaatcag aatcattgaa gtagtggaag atgtaaatga aaaacaaaaa gtaaagattt
     9781 tagcaaaaat tgaagcccat tttaaaggag agtctctacg cggaaaaaca tttgctgttt
     9841 ggggtcttgc ctttaaacca gacactgacg atatgcgcga agctccctct atcccaatca
     9901 taacagaatt gcatagacta ggtgtaaact tacaagtcta tgatccagag gcggaagaaa
     9961 catctaaata ttacttcaat ggaaaagtaa aatatgcaac gggtgcgtat gaagccttgg
    10021 aaggagctga agcactacta ctcttaactg aatggagaga atttagagaa cctgatttcg
    10081 gaagagttaa aaatctttta accaagcatg taatttttga cggtagaaat ttatacaaga
    10141 aaccacaaat gaagagactt ggttttactc actatggaat cggaatgaat taaaaatttt
    10201 tacaatgcac aagaacacgc cttgatatcc gatggacttg aaaaatttac atcgaaggct
    10261 ggacttgtgc aaagccatct tcctggattg atataacatc ctgctccttg agcacacggt
    10321 tgtggtgaaa cagctcccga acctagacct aatacatcta aaatcatctg gcagttacct
    10381 gtattcgttc caataaatgt atagcctgga gaaacacctc cacgtcctat gcatgtagta
    10441 ttacaattta cattcggaca tccaatatac caaatatatc ctgctgacaa aattcctggt
    10501 ccgggtgaac aagtaagtgt agatacattt aaaaccgaat agattgcttt gtttccataa
    10561 gtatccattg ctaacactgt aatataatag ggcatattca tagctaaccc attaatcgaa
    10621 aaagaggata gattggatat ccaattctga gcgatagttc cattggtctc tgccatagaa
    10681 atagtggtta tattgtttga taaggagtat acaaccttat actgtagatt gctttggggt
    10741 gtagaattat cggttgccga aacccatcca acggtgatgg atgtgctagt aacagtgttt
    10801 gcagagagaa ttcccgaatt gccaggaatt ggtggcattg tatcttgagt ggtaagccca
    10861 gcgataggta aataggctgc tttatttcct tgtgcatccc ttgctataac tgaaaaataa
    10921 taatttgtct gaggaactaa gttaattact gaaacctgtg tcaaattgga agtaaatgtt
    10981 ccaaccggca taccatttgt ttcaatattt gaaactgtat tcaaattact acttaaagaa
    11041 aaataaggaa gatactgaat attgctttgt acagtgaaat catccgtcgc ggcagtccaa
    11101 ccaattgtta tagaagaaga actaacgttt aaaatagaga ttgctccaac ggtgggtggc
    11161 gctgtatccg atgcgttcgt tgcgggcgtt gagctagtag atcggagaac cctgtctata
    11221 taggaaagga tggcggtatt ggacctatct tcaatttcat aattacaatg ggatagaagt
    11281 gctaccaaca tcatagttaa taaaatgaaa atagttttct tcatatattc ttttgacccc
    11341 cgctattaaa aacaaagtac gctaaatcta ctgcctaata attcaagcct gtttttcatt
    11401 ttattcattc ttgttaataa cctattgtcg tttagctgtc cgattgttaa ttcattagca
    11461 atcggaactg cttttaaata ttagattctt ggctataaaa attttggaag ttaaaactcc
    11521 gatccgctta tgtttttact agcaaacttc aaatatgtat ttctttttaa aaatagattc
    11581 cgataatggt attaaaatcc aagacaggtg acagaatcat gaaccaattt atatctttaa
    11641 ctctatttgc atttctaatt acatctggaa tttctgcagt agaaagaatt caacaagaga
    11701 attctaaagt aatcgatgag tttgcaaaaa cagaagaatt tattaaaaac agaaaaatct
    11761 ccaaagaata tagaaaaaaa accttagaaa agaatcttac caatgcaatc aaatatacta
    11821 tgcacaaaaa gtttcctgac taccaagcta gaatcaagga tctgaagcca gatgctgtaa
    11881 agtatgaaca agaaaaagga acttttaatt actatctaaa ctacaaagac tactatattt
    11941 tttacaactt tgcagttgat ccggaaattt atctacaact cccagcagac gaaaaaatgt
    12001 atattaagtt agcagatcaa gataaagaaa ctgcttctaa ccctcctcct gctccagctg
    12061 gaaacgcagg aaatgacaag ggacccaaca aaccaatcgg aacaaatcct taattcaatt
    12121 gtttgataaa aactcaaaag aatttcatct agctatctct tctgttttag aagcgggaaa
    12181 gatagttaga tcaatctacg acactaactt cgaagtaagt tggaaagcaa aagatgatcc
    12241 actcacagaa gcggatttgc gagccaacca agaaatcatt cgtcaaattc agctgacttt
    12301 tcctgaagat gcaattttat ccgaggaaat ttcagattcg gaaaaaagac tttctaagga
    12361 aagggtttgg attatagatc caatcgatgg aaccagagaa tttgtaaaaa agaatcctga
    12421 attttccgtt agtcttggtc tcagtgtttc cggtaaagcc tttattggat ccatactaaa
    12481 cccaattaca agagaattgt ttgtaggaat tgttggtcaa ggcattgcat atttagttct
    12541 aaaagatgac ttctccttag atctaaatca gttgaatctt accaagcatg acttcaagcc
    12601 aatggattct aaacctttta cctatgtttc tagatccgaa ttttttaaat ataaattgtt
    12661 cgaccaaaat ccctactgga aagaaaacta tgatttaaaa cctgtaggct ccattgcgta
    12721 taaattagct ctagcagcag cgaatctagg cgacttagcc ctatctctaa aaccgaaaag
    12781 tgaatgggat atatgcggtg gtgtagctct cattgaagca tctggtggga ttgcagttga
    12841 tttgaaaaac caaaaagctt ttccatttaa cgagaaaagt cccttacaac atggaattct
    12901 agccggaaat ccattcttag tagaaaagat tttaaaagaa aaaggcaact tctttcgaga
    12961 ctctttagtc aattgggaat aattttctaa ttgaaatttg tatagaatac gaactttctt
    13021 ttccatggca tccgtaatac ttgactatcc agagttattt caatttgtaa gacctatctc
    13081 tctggactcc tagcatgccc tatgcgagat tgactctaat ctagaaaaga cagaactcat
    13141 acagggagtg atcattgaaa aaaggacgaa aagcccacag tataattttt ttccaatgta
    13201 ttagaggaag aattgggtaa gattaaacca cagggaagca ttttactcat cgaaaagcca
    13261 atttctctcc tcagctcaga gccagaacca gatattgcga tagtaaagga tccttttaca
    13321 actttaaatt taaaaaccca gaaacagccc ttttagtagt agaaattgca aaaagttcaa
    13381 tctcactaga tcgtgtaaaa aaagaaattt atgcttccgc aggagttcct gaatactgga
    13441 ttgtaaattt aaaaagtaaa gaaattgaag tctactcaga gccaatggaa ggaaaatacg
    13501 aaaaatccaa agtgcatgga aaagattcaa agatctaaat ctttcaatca taacttgact
    13561 taaaaccctt cttcgatgaa tagtgggact ttaaaaaaat gagttctatt taaaaatatt
    13621 ttcgccacct atgcacggtt caactgcgct caccgcaagt ctatggtaat cttttaattt
    13681 ttctgtcata tcgaactggc ttaaaaataa aaaagcccac tgtagtagcg ggcttttaca
    13741 caattctgaa caatttattt agaattaaga tttgtttgct agcgttactc ctggctttgt
    13801 tgtaacgatc tcgatttttc tgagtgcttt ttctggatcg attaatttac ctttgtaaat
    13861 tacttcaaaa tgtaagtgag caccggttgc tgttccagtt ctaccaactg aaccgattac
    13921 cttacccatc ttaacaaaat cgcctacttc tacaacggtt gtagaacagt gtgcatatac
    13981 agttttatag ccacctttgt ggcgaatgat tacagtatta ccataaccac cgtttctacc
    14041 tttaaactct acaacaccct ctgcagatgc aatcacagga gtgccaacag gagccgaaag
    14101 atctagacct gaatgataat ttcgaacact aggattaaaa ggatctcttc tataaccaaa
    14161 tcgggaagtt accttaccgg ttaaaacagg aagagcaaat accttcattt tataaatggt
    14221 tccaccagcc atctgagatg tagttggttt tgagctattg attcttagaa cttgtccaat
    14281 gcgaatcaaa tctcctttta aaccattgag ttttttaatc tgctctacct tcattttata
    14341 tctgcgagcg attaaactta aagtgtcttt agcttgcact ttatatccgc ctactactgt
    14401 cgtagttggc ttgataactg tagcagaaat aactctagca atctgtatat gagacggaag
    14461 cttataattg gaagaaatcc ttgaataggt ccctacactt ttgtttgagt cctctgcagt
    14521 ctcctcgtct ggagaaaaaa gttcctttaa ttgagagtct tccccttcat aatattgacc
    14581 agactctgag tcaatatatt cctttaattc gttctcaaaa gaatcaaatg gacttgcgaa
    14641 agcgactgac gtgaatatga ttgtaatcgc gaaaactatt aatatccaga attgtataag
    14701 catctaatct atgtatcgta taagtaataa aaaatagtca agcaaaatcc ctattttttt
    14761 gcctttttat ctttacttac gtcctttgca tcataataaa ctagcttttc agctatcaac
    14821 tgatccaaat actctactgt aaattttttg ttcttaaagt cttcgttgat gatatatgtt
    14881 accatccgac caacagcact ttctctatct tcattgctat tgtaacactt gacttttaaa
    14941 atggacgcag ttgactctat ccatattttt acagaggtat cctttttgat tatgtctttg
    15001 ttgtctagaa caagattctc tttcacatag tatgtatttt gcgaataatg ttcattaatc
    15061 agtgttaaat catctttttt aatcagacgc ttattacaag ccaaaatgaa aaaaattgac
    15121 aaaaaaatga aaattgatct aaacattatc cgcactttac tttatcttct caactacatc
    15181 cagtagtaga tccacatgaa ttacatttca agcaagatcc attccttacc atctgaaaag
    15241 atccgcattc tacacaggaa tctcctgtat agccttggat ttttgctttt tccctttgcc
    15301 cgattgcatc tagaaccgca atgcttgctt cctttttgag taaagcagaa tcatttcggc
    15361 tgataacatc agaaagagag ataggcattg gtttcattcc attcgaagaa ggcttattct
    15421 cagagatttc agagagggat tcattcatct ccgaatctaa cctcccgggt tttcccactt
    15481 catcccctct caactcttca tctggaacta cttgtcctag atcgtatctt cctagatagg
    15541 taattgcgag ttcacggaag atataatcga ttacagaggt actcatcttg atatgttgat
    15601 ttccattgac cataccattt ggctcaaatt tgaagaatgt aaatgcttct acaaactctt
    15661 caagcggaac tccatgttgt aatccgagag aaacagcgat tgcaaatgcg ttcatcaaac
    15721 ttctaaaagc tgctccttcc ttatgcatat caataaagat ttcacctaac tgtccatctt
    15781 catattcacc tgttctcaga taaactttgt ggcttccaac ggttgccttt tgtgtgtaac
    15841 ccgctcttct agaaggaagt tttcttcttt gagaaatata cttcagaaca attttttctg
    15901 tggttttgat gattggtggt ttctcattgt cttcctctga aacctcttcc tctgcaaagt
    15961 gtagcatttc actcacagaa tttaatggtt gagagagttt agatccatct cggtataaag
    16021 catttgcttt taccatgagt ttccaagaaa tcaaatacgc atctttcaca tcttctagag
    16081 ttgcttcttc tggaagattg atggttttag aaatggcacc cgagataaag ggttgagcag
    16141 cagccatggt tcgaatatga gattggtagg ataagaagcg tttgccgtat ctaccgcact
    16201 tattcgcaca atcaaaaaca ggaagatct
//
LOCUS       DAOQOS010000119        16068 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_1835_length_16068_cov_5.782989, whole genome shotgun sequence.
ACCESSION   DAOQOS010000119 DAOQOS010000000
VERSION     DAOQOS010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 16068)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16068)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16068
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1835_length_16068_cov_5.782989"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..524
                     /locus_tag="PLF66_20240"
     CDS             <1..524
                     /locus_tag="PLF66_20240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66431.1"
                     /translation="VMFSGVGDSWNGGNYWTSDSEGSSHAVVDKSIVVNYYEYGKMRV
                     RCLPNLPDKSLIDLEPKAIDKKNWADLGDRNRISWYGAKLKCFGSEFRLPTRTELLAA
                     HLAGLTRIWFLETKQKTSSHYWTSEEYSLGRAHLMHHQFHVNAQKWSPIKDPFKIIHW
                     DGVTTFHAKFIRK"
     gene            complement(606..968)
                     /locus_tag="PLF66_20245"
     CDS             complement(606..968)
                     /locus_tag="PLF66_20245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66432.1"
                     /translation="MQIRILLILFLSVFGFNCVSGEMKVPTRRVLSQIPSNKPGVIYR
                     FYIDLEEKSATKNNIFGVERVYQGNDSSTGEAFFKFYFYQEAGIELCTYNDSAKNYEC
                     KDLEIIFKQKNEMPPVKE"
     gene            complement(959..1369)
                     /locus_tag="PLF66_20250"
     CDS             complement(959..1369)
                     /locus_tag="PLF66_20250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66433.1"
                     /translation="MKIYLNKIFLFLFFVFGFVNCNNEIYVKHVYTDFKKIGSPPNQP
                     TNTYEITRVTTIRTAETINKARIKNIYYYTLPPILKLVRFALSTGESGIVFLEDPEAE
                     ENSKVVLFKPAPDKYVMVTKMLCIINEEEALECK"
     gene            complement(1350..1673)
                     /locus_tag="PLF66_20255"
     CDS             complement(1350..1673)
                     /locus_tag="PLF66_20255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66434.1"
                     /translation="MKTFFLSILILFHLFCASEPPVKENTPDNYFALRDITLGKDNTL
                     YLSVRSAYIEKDKIKEAFEEFQNLHSENKIKRTYWKCKVILNSTLECTEELKIKDIAD
                     ENLSK"
     gene            complement(1816..3525)
                     /locus_tag="PLF66_20260"
     CDS             complement(1816..3525)
                     /locus_tag="PLF66_20260"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HMZ66435.1"
                     /translation="MPNKKIYKDKEYIQRILEEAARDGKTEIVETLIENGAEINNKKY
                     SPLIAAFQNKQMEVVKLILDKGADVNLVANGTSALREAAQLGDFELVKFLVEKGAAIN
                     FKDRYRNSPLSYAIFGSGFGKVPEYSEERKSIIKFLIENKADTTDIDLLRYSYSEEQF
                     STEQIKFLLDSGIPVDSKKSNSDFLEDFHSSLEKACLSEKVNLGIIKLLIERGANPNP
                     KTDSKFGSSLLNTVIYKIHEHKRKGEKLQIRYTKICLLLIQAGADILAKYKYGRNPIT
                     SAIASDNLGILMYMFRGRKSDILKDPRSKNLIHTALSYGNKVILQFLIKKGLDVNSLD
                     ENGLTPLHTALRKFYHDHINVKYMAHSELEDRLEPIRILLKNGADVKRRKKNHSSPLH
                     YAVNTDIPHIFQLILDHGADINEKGKFGYTALHYAANHSCLNSLAFLLQKGFDINQKD
                     DFGFTPLHHAGYNGLTETVKFLLSNGADCHLTDNYGNTPLHYAARQGRLSQIEKVKPN
                     PEPSAIPILKIETVKLLIENGANSELENNKGETPLDLANRRGHKEIIELLRTQNLSKK
                     RKL"
     gene            complement(3603..4169)
                     /locus_tag="PLF66_20265"
     CDS             complement(3603..4169)
                     /locus_tag="PLF66_20265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66436.1"
                     /translation="MTLFKKQYRVESSRRLGWDYSENGYYFITICTKDSECLFGEIQN
                     GKMRFNEFGIVVQEEWEKSFVIRRELSCNTYIIMPNHIHGIIVLDNVIPVETHGRASL
                     HDDASLQHKMVIPYRSPKSISSFVAGFKSVATMRINQIRNTPKIPVWQSRFHDRIIRD
                     EIELYNVKNYIENNPINWEEDEFYADAA"
     gene            complement(4345..4605)
                     /locus_tag="PLF66_20270"
     CDS             complement(4345..4605)
                     /locus_tag="PLF66_20270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66437.1"
                     /translation="MNNNENLKGENFKEVDRESPVKFKPLTEEEIKTLSPEESEKNRE
                     WVIKSLKDLNASIIKDANDLDRKLKELREKKRREQMNEESSE"
     gene            complement(4763..6082)
                     /locus_tag="PLF66_20275"
     CDS             complement(4763..6082)
                     /locus_tag="PLF66_20275"
                     /inference="COORDINATES: protein motif:HMM:NF021622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66438.1"
                     /translation="MSELKVLRENNGSKKGNDWIGQIININLNLFADGHERIFQENEK
                     FNREKKPCFSISKGMNSERSILTSYFIGVDWIEENVQAVYVEPKLNKDNKEQTNYLQM
                     LFSALKHPEVSSHTEDLFEIKFDKPYIEIDSQQDLLTPLLIVQFLQIVKQIVRKGLKK
                     SYYKVENNLYSKVKGKLLVSKTIKQNTFKNKTLHSYCSYDEFGVNCLENRLLKKALVF
                     IRSYLPKIKNLNAGQFIENTFNYILPAFNDVSEDVEINTIKHSKANAFFKEYSEGIRL
                     AKLILKRFGYNIVNTQSEKIKVPPFWIDMSKLFELYVLGLLKDRFGRAVTYHFTTNRN
                     ELDYLLNTDELKIVIDAKYKLAYNNSSIIEDMRQISGYARLKSVYKELDIAPKSNRQP
                     PIIDCLIIFPDQNNGQEDIKIECLKTKKISRFMNFFKYSIKLPLIKK"
     gene            complement(6075..7724)
                     /locus_tag="PLF66_20280"
     CDS             complement(6075..7724)
                     /locus_tag="PLF66_20280"
                     /inference="COORDINATES: protein motif:HMM:NF019348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HMZ66439.1"
                     /translation="MAEKKYWAGGFGEPEVLNDFILNNYWAALKYDSPDQSSTAKSAF
                     ENFDKIKKGDWFAIKGFSPQKIKIHYIGEVISKDDYKKSISFKSWGIEKFELSGRPRT
                     GRWPDTLIEVTDGEAIQEIFFKRWPMNAYTKLLEHKHQIILQGPPGTGKTRVAKEIAY
                     KLIKDDILPENEGERKKKIKELEDSGNFKLIQFHPAYSYEDFVRGITAKSNGNNIEYK
                     TENKVLGEFAEKAYKNLEDSKKDVTKLSEEKQLEKYLEELVDEIQETLDEKGKFPISK
                     SAYIFEIDEDAFRYKGDNWATVFRMPFSEIIKLYNFGIAERKEIKKQSNLSGRTKQHA
                     TYFFNFLLKFKDHVNKNKNQDPKMDSFGKIDKQNFVLIIDEINRANLPAVLGELIYAL
                     EYRGESVESMYEIDEDRKITLPENLYIIGTMNTADRSVGHIDYAIRRRFAFMDVIPSG
                     DIIKQVISDPNLLSKAITLYENVEKLFIESTSDSNSEKKYLASDFSEKDVQLGHSYFL
                     AKTEDELKLKLDYEIKPILREYVKDGILKEEAKKVIEGLNV"
     gene            complement(8010..8708)
                     /locus_tag="PLF66_20285"
     CDS             complement(8010..8708)
                     /locus_tag="PLF66_20285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66440.1"
                     /translation="MKYLLIISLLFIIGCSSTLERAPAPAEETETPKKETKPEHTQEE
                     IDAIDQKLEKLKTEPIKKLIENVGKASTNYERKASREAIQTVLDRKLLVYLGKEFPDT
                     KDVLAYKIYNPTGEENYSGLELKDLFMLYLGQNPNTGSKIEKQNLDMKRNKKDKNVKI
                     LLQPPGAREMQEFYFKIFNDKLILSGYRSGTQLNPTGYEYVVSEIFDQLSCKEMGYMP
                     RLDFINQKLVDSIK"
     gene            8878..9813
                     /locus_tag="PLF66_20290"
     CDS             8878..9813
                     /locus_tag="PLF66_20290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66441.1"
                     /translation="MKNKKNLTASEQLILAIEFLSLSEEYIIRNKKLNSIHLAEFLDE
                     LQDPERERNDTEWSPDRKFRLYLTKLRNIGISLNLKTKTIQFDKNVNLFKILSIYVKY
                     FCEDYTEQHLRLFYKSKKENFFESLRLFVFIRYAIQYSLPIELSFLKTMDWKPSFRRV
                     IPRYLASKNQLLDVVVTDLKDNVNKWFSLVNILSIENDLYSAFRKRNKRNLPFDRASF
                     ESSPEGRFHWANIVYTVQFNSYTFNHCINTNELDYKIIKNESPLQMIVEITCNDSYLI
                     QRILFNYGVYVKLLAPSEAVSQFKNKLSALEKHYR"
     gene            10066..10386
                     /locus_tag="PLF66_20295"
     CDS             10066..10386
                     /locus_tag="PLF66_20295"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HMZ66442.1"
                     /translation="MPSQIKNFYTIEEIKKNLSEYFYDKPVRKAYIFGSYARGEANEL
                     SDMDILVELDFTEPIGLYFFTMEYELKKIFNQKIDLITIEEISPFIKPYIDKDKILVY
                     ERKE"
     gene            10370..10726
                     /locus_tag="PLF66_20300"
     CDS             10370..10726
                     /locus_tag="PLF66_20300"
                     /inference="COORDINATES: protein motif:HMM:NF014043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HMZ66443.1"
                     /translation="MKEKNKITDRVRISIVLEAIQDIESFMHSISQENFLSNRLVQSA
                     CAKKLQDLCNASEDISDEIKIKNPDIPWSQMDGIRNRIAHEYFGINYKIIWTVIQKDL
                     PEIKINFEKILPSLED"
     gene            complement(10790..11314)
                     /locus_tag="PLF66_20305"
     CDS             complement(10790..11314)
                     /locus_tag="PLF66_20305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HMZ66444.1"
                     /translation="MIRLIILFLLSFGILSSETTQDKIGFTVKHKFKTVNGKVQKFSI
                     NNLSLKKEANRYSAQPFSIQFKLLDMRTGDPNRDSHMLEVLGYPEQESIELQITKVEE
                     IKPKEEYKIYLDAKINGVTQSLESIATTVEKEGKVQIKGQLKILLDQFKIKAPSLLFF
                     PIENTVLCEYEFNV"
     gene            complement(11328..12071)
                     /locus_tag="PLF66_20310"
     CDS             complement(11328..12071)
                     /locus_tag="PLF66_20310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016544914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66445.1"
                     /translation="MYSKLILFILFLFSFLTLPAESFYDNLNPNYTNLFGNIRPVSYW
                     DGGSINFNFPQQNSFLVESNAMNPKPESSPVLNKKQIERRRSFLEWHQITGFATWGLW
                     LATNIAGEKAANTYKKEYEPIANVVLLANPSQNALFYYGIMQVSPYDSKGGATHGSLA
                     GATFLMYSITAGLAFFSPSKMQADREEGWSTIFTHKAAIFVHLPSMLALASLGSQVQK
                     GGPHVINEMQTIGWVGFGALSLSIATFYF"
     gene            12455..13951
                     /locus_tag="PLF66_20315"
     CDS             12455..13951
                     /locus_tag="PLF66_20315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66446.1"
                     /translation="MLTKRERQYLEHKLKYNKALLFMLLSIFPLVFLPMILDLQIFRN
                     LLSFKTISFFLILFFLVGAFYDVFTAKIHWNDRNIIEEIGKIKVIEGEQVSIFWSDHV
                     LTFPKTWDINHIKTLFAKDVKIKYYHLKVFPSFKKFKFAINCVLSLDEFSVEEDIKKE
                     KDWRDSFSQLFHLPFLILFIGFGIMSSEVKEDLDARFSKEQNIEIEKVTQLSMVSASI
                     QEILNEKYPFPVFVKLEKILFIEENFIHWIIPISEDDLIQLRKDFTELEERQNLIQSI
                     LDKNLNDIKKIKKQDLKVLENNPFLSDFLETLRKYDSRTIKLLFEIDLNQKDLRSFPL
                     LSETQKENMIEIQKKGILSFSNFEVDFFKTKMQTSYDFIISKKQKIANDYFLSQSTWS
                     VPQINRSDLFAMDQTKSSLWKKFYTESLENKNSIQCYIFKKEGRYILIPKDFSQNDLG
                     DFLYLKREYAFFLNSSFLFLILESIPVFLFLLDHFKRRIAPSLKIKNY"
     gene            14398..14775
                     /locus_tag="PLF66_20320"
     CDS             14398..14775
                     /locus_tag="PLF66_20320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001057833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HMZ66447.1"
                     /translation="MGSAPENLGPIIIQLLLGIGFAALILSLAFLLNPKKRSKPHDTF
                     ECGVPYYGDARGVFNIKFYLVAVLFILFDIEAVFLYPWAVNLGKFTEAGLGGFILIEM
                     LLFIFILVVGLYYIWKKGALEWD"
     gene            14766..15326
                     /locus_tag="PLF66_20325"
     CDS             14766..15326
                     /locus_tag="PLF66_20325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002630731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030964 - NADH dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
                     /protein_id="HMZ66448.1"
                     /translation="MGLNEILSKPGQMLGDMAQVANLDAVVNWGKSYSLWPYPFATAC
                     CGIEFMGTACSDNDIARFGAERPSFSPRQSDMILVLGTITYKMAPVLREIYDQLCEPK
                     YVISVGACASSGGMFDTYGVVQGVDRFLPVDIYVPGCPPRPEAIMDALVKLQKKVQTQ
                     GLEARRQEVMRKIDEINEKNKPMIVR"
     gene            15323..15844
                     /locus_tag="PLF66_20330"
     CDS             15323..15844
                     /locus_tag="PLF66_20330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008594213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HMZ66449.1"
                     /translation="MKEKVESFLKSKLASFIFKEEAVNSNIPTFFIKSEGIFPVIKAL
                     KEDKELKFDYLNDLTAVDWLGKKTPRFEVCYLLRSLSNKHTRIMLKVPIEEGETIPSI
                     VPIFKGANWPEREVFDMFGIEFTGHPQMERILLPDNFQGHPLRKDYPLEGFGQDYLIA
                     DLLQIHQEDKEAN"
     gene            15853..>16068
                     /locus_tag="PLF66_20335"
     CDS             15853..>16068
                     /locus_tag="PLF66_20335"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004779766.1"
                     /note="Catalyzes the transfer of electrons from NADH to
                     quinone; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HMZ66450.1"
                     /translation="MYDKTAQKFKGKFDNLPEGHLLVNLGPSHPATHGILQNVIQIDG
                     ERIVEAEAIIGYVHRCFEKLGERYDYNQ"
BASE COUNT         4974 a         2637 c         2590 g         5867 t
ORIGIN      
        1 ccgttatgtt ttcaggagtt ggagattcat ggaatggtgg aaattactgg acctctgatt
       61 cagaaggcag ttcacatgca gtggttgata agtcgatagt cgtaaactac tacgagtatg
      121 gcaaaatgag ggttcgctgt cttcccaatc ttcctgacaa atctttgatc gatttggaac
      181 cgaaagccat cgataagaag aattgggctg atctaggtga taggaataga ataagctggt
      241 atggagccaa gctaaaatgt tttggctctg agtttagatt gccaacaaga acagagttat
      301 tggctgctca cttagctgga ttgactcgaa tctggttttt ggaaaccaag caaaaaactt
      361 cgagtcatta ttggacttct gaagaatatt ctttaggaag agcgcattta atgcaccacc
      421 aatttcatgt caatgcacag aaatggtcac ctatcaaaga tccatttaaa attattcatt
      481 gggatggtgt tacaactttt catgcaaaat ttattagaaa ataaactaag cattgttgag
      541 catcatagta atctatgaga actagatacg ataggtgaaa ttggaaagga atactttcaa
      601 atcatttatt cttttacagg aggcatttca tttttttgtt taaaaataat ttctaagtct
      661 ttgcattcat agtttttggc tgaatcatta tatgtgcaaa gttcaattcc cgcttcttgg
      721 taaaaataga atttaaaaaa agcttctccg gtggaggaat catttccctg gtagactctt
      781 tctactccga aaatattgtt tttagtagca cttttttctt ctaagtcaat atagaaacga
      841 tagatgacac ctggcttgtt gcttggaatt tgcgaaagaa ctctgcgtgt gggaactttc
      901 atttcaccac ttacacaatt gaatccgaaa acagataaga agagaatgag tagaattctt
      961 atttgcattc tagggcttcc tcctcattga taatacaaag cattttggtt accatgacgt
     1021 atttatccgg agctggttta aaaaggacta ctttgctatt ttcttctgct tctggatctt
     1081 ctagaaaaac gattcccgat tcaccagtcg atagagcgaa tcgaaccagt tttaaaatag
     1141 gaggtagagt gtagtaataa atatttttaa tcctagcctt gttgatagtt tccgcggttc
     1201 gaatcgttgt gactcgagtg atttcgtagg tattggtggg ttgattcgga ggacttccta
     1261 tttttttaaa gtcagtatag acatgcttaa cgtatatttc attgttacaa ttcacaaatc
     1321 cgaaaacgaa aaagaggaaa aggaatattt tatttagata gattttcatc tgcaatatcc
     1381 ttaattttca attcttcagt gcattctaat gttgagttta agattacttt acatttccaa
     1441 taggttcttt tgattttatt ttcagaatgt aaattttgaa attcttcgaa ggcttctttt
     1501 attttatctt tttcgatata agcagatcga acggaaagat agagagtatt atccttacct
     1561 aacgtaatat ccctaagcgc aaagtagttg tctggagtat tttctttgac gggtggttca
     1621 gaagcacaga atagatggaa tagaattaga atagatagaa aaaacgtttt catgcaggca
     1681 tgactatatt ttagtcaatt tattttgtaa ataaaaattt aaagtaaatt acgtgagttc
     1741 aaaaaagtaa gaaagaagaa aatcctccgg ctaggtgttt caataagatt attttttatt
     1801 taccagaacc aatgttcata actttctttt ttttgagagg ttttgagttc gtaataattc
     1861 tataatttct ttgtgacctc ttcgatttgc gagatccaat ggagtttcac ctttattgtt
     1921 ttccaactct gaattggcgc cattttcaat taagagtttt actgtttcta tttttagaat
     1981 aggaatagca gaaggttctg gatttggttt tactttttca atttgcgata atcttccttg
     2041 tcgtgccgca taatgaaggg gagtattacc ataattatcc gttagatgac aatcagcacc
     2101 attactgaga agaaatttta ccgtttccgt gagtccgtta taacctgcat gatgaagtgg
     2161 agtaaatcca aaatcgtctt tttgatttat atcaaatcct ttttgtaaaa gaaatgcaag
     2221 agaattcaaa caagaatgat ttgccgcata gtgaagtgca gtgtatccaa actttccttt
     2281 ttcatttatg tctgcgccgt gatctaaaat caattgaaat atatgaggta tatcagtatt
     2341 aactgcataa tgaagaggac tgctgtgatt tttttttctt ctcttaacgt ctgctccatt
     2401 ttttaaaaga atccttatag gctctaaccg atcctcaagc tcagaatgtg ccatatattt
     2461 gacgttgata tgatcatggt aaaatttcct aagtgcagta tgtaaaggag tcaatccatt
     2521 ttcatctaag gagttgacgt ctaagccctt tttaattaag aactgaagaa ttaccttatt
     2581 tccgtaagat agagcagtat gaatcagatt tttacttcgc gggtctttta gaatatcaga
     2641 cttcctacct ctaaacatat acatgagtat accaagattg tcagaggcta ttgccgatgt
     2701 gattggattt ctcccatatt tatatttagc taagatatcg gctccagctt gaattagaag
     2761 gaggcagatc tttgtatacc ttatctgtaa tttttctcct ttccgcttat gttcgtgtat
     2821 cttatatatc acagtattga gcaacgagct accgaacttt gaatcggtct ttggatttgg
     2881 attagctcct cgttcaatca agagttttat aattcctaaa ttaacctttt cgcttaaaca
     2941 agccttttcc aatgaactgt gaaagtcttc tagaaaatca ctatttgatt ttttgctatc
     3001 cacaggaata ccactgtcta aaagaaattt aatttgttct gtgctaaatt gttcttcgct
     3061 atagctatag cgtaataaat caatgtcagt tgtatcagct ttattttcga tcaagaattt
     3121 aataatactt ttacgctctt ctgaatactc cggaacctta ccaaaccctg aaccaaagat
     3181 ggcataggat aacggagagt ttctgtatct atctttaaaa ttgattgcag cacccttttc
     3241 tactagaaat ttaacaagct caaagtcacc gagttgagca gcttctctta acgctgaagt
     3301 accgttagcg acaagattca catctgctcc tttatccagt ataagtttta caacttccat
     3361 ttgtttattt tgaaaagctg caattaaagg agaatatttc ttattattta tttccgcccc
     3421 attctctatt aaagtttcca caatttccgt tttcccatct cgtgctgctt cttctaaaat
     3481 tctttggatg tattctttgt ctttgtaaat ttttttgttt ggcattggtt ttgattctcc
     3541 tgtatgatgt cgttggctaa ttcgtattaa ccgcgtttct tttgtgatgt tgtgggtgaa
     3601 ttttatgctg catccgcata aaattcatct tcttcccaat tgattgggtt attctctata
     3661 tagtttttta cattgtataa ttctatttca tcgcgaatga tccggtcgtg gaatcgcgat
     3721 tgccataccg gtattttggg tgtatttcga atttgattga tccgcattgt tgcaaccgat
     3781 ttgaatccag ccacaaaaga tgaaattgat tttggagaac gatatgggat aaccattttg
     3841 tgttgtaacg atgcatcatc atgtagagac gcacggccgt gcgtctctac gggtataacg
     3901 ttatccaata caataattcc atgtatatga tttggcataa taatatatgt attacacgac
     3961 aattcccttc taattacaaa tgatttctcc cattcttctt gaacaacgat tccaaattca
     4021 ttgaatctca tttttccatt ttgaatttca ccaaataaac attcgctatc ttttgtgcaa
     4081 atcgtgatga aataatatcc attttctgaa taatcccaac ctagtctgcg actggattcg
     4141 actctgtatt gttttttgaa taaggtcaat tctgtgttcc ttgttacggc taatttagat
     4201 taaccccaac tcttctgcta tgtcatgggt gagtttggag attaaaatta tcggcaaata
     4261 tgagcgcaaa agaatttcca actcttctgc tatgtcatgg gtgagtttgg agagggggcg
     4321 tttggattgt ttatttaaaa tgatttattc actcgattct tcattcattt gttctcttcg
     4381 cttcttttct ctcaattctt ttaattttct atccaaatca ttagcgtctt taatgataga
     4441 agcatttaaa tctttaagag acttaataac ccattctcta tttttttctg attcttctgg
     4501 ggataatgtt tttatttctt cttctgttaa aggtttaaat ttaaccggcg attctctatc
     4561 cacttcttta aaattttcac cctttaaatt ttcgttatta ttcatatagc tctttccact
     4621 tcgctgattc cataatcagc ttttataata ctgaaattat atctgattct actttcatta
     4681 tccggaaata ttttttccga tttaaaaaaa gttaatctat attaacccca actcttctgc
     4741 tatgtcatgg gtgagtttta cattattttt taatcaatgg tagttttata gaatatttaa
     4801 aaaaattcat aaaccgacta attttcttag tttttaaaca ctcaatcttt atatcttctt
     4861 gtccattatt ttgatctgga aaaataatta gacaatcgat aataggaggc tgcctatttg
     4921 atttgggagc aatatctaat tccttataga cactctttag tcttgcgtaa ccactgattt
     4981 gtctcatatc ttcaattata gatgaattgt tataagccaa tttatatttt gcatctataa
     5041 ctatttttaa ttcatctgta ttaagtaaat aatctaattc atttcggttt gtcgtaaaat
     5101 gataagtaac cgctcttcca aatctatctt ttagcaatcc caaaacatat aactcaaaca
     5161 gcttactcat atcaatccaa aagggaggaa cttttatttt ttcactttgc gtattaacga
     5221 tattataccc aaatcttttt agtatcagtt tagcaagtcg aatcccttca ctgtattctt
     5281 taaagaaggc atttgctttc gaatgtttta ttgtattaat ctcaacatct tctgaaacat
     5341 cattaaatgc tggtagaata taattaaaag tattttctat aaattgtccg gcatttagat
     5401 ttttaatttt cggtaaataa cttctgataa atacaagagc cttctttaat aagcggtttt
     5461 ctaaacagtt tacgccaaat tcatcgtagc tacaatagga atgaagagtt ttattcttaa
     5521 aagtattttg cttaatggtt tttgaaacta aaagctttcc ttttactttt gagtataaat
     5581 tattttcaac tttgtagtaa gattttttta atcctttcct tactatttgt ttaacaatct
     5641 gtaaaaactg aacaattaac aaaggggtaa gtaagtcttg ttgcgaatct atttctatat
     5701 aaggcttatc aaatttaatt tcaaataaat cttcagtatg gctcgaaacc tctggatgct
     5761 ttaaagcaga aaaaagcatt tgcaaatagt ttgtttgttc tttattatcc ttatttagtt
     5821 taggttcgac gtaaaccgcc tgaacatttt cttcaatcca atcaacgcca atgaaataac
     5881 ttgtgagaat tgatcgttcg gaattcattc ctttagaaat agagaagcaa ggtttctttt
     5941 ctcgattgaa tttttcattt tcttgaaata ttctttcgtg accatcggca aataagttaa
     6001 ggttaatatt aattatctgc ccaatccagt catttccttt tttacttcca ttattctccc
     6061 taagaacttt gagttcagac atttaaaccc tctattactt tttttgcttc ttctttgagg
     6121 attccgtctt ttacgtattc tcttagaatt ggttttattt cataatctaa cttcaacttt
     6181 aattcatctt cagtctttgc taaaaaatag ctatgaccga gttgaacatc tttctcacta
     6241 aaatcagatg ctaaatattt tttttcagaa tttgaatcag atgtactctc aataaatagt
     6301 ttttcaacat tttcatataa tgtaattgcc ttgctaagaa gatttgggtc tgatattact
     6361 tgcttaatta tatctcctga cggtattaca tccataaacg caaatcttct tcttatcgca
     6421 taatcaatat gacccactga acgatctgca gtattcattg ttccaatgat ataaagattt
     6481 tccggtaatg taatttttct atcctcatca atttcataca tgctctccac tgattctcct
     6541 ctgtattcga gtgcataaat taattcgccg agcacagcag gaagattggc ccgattgatt
     6601 tcatcaatga tgagaacgaa attttgtttg tcgatttttc caaacgaatc cattttagga
     6661 tcttggtttt tgtttttatt tacatgatct ttaaatttta gaagaaaatt aaaaaaataa
     6721 gttgcgtgtt gttttgttcg tccacttaaa ttactttgtt ttttaatctc ttttcgctct
     6781 gctattccga aattgtaaag tttaataatt tcggaaaatg gcatgcggaa aacggttgcc
     6841 caattgtcgc ctttataacg aaaagcatct tcatctattt caaaaatata tgctgatttg
     6901 ctaataggaa atttaccttt ctcatctagg gtttcttgaa tttcgtcgac taactcttct
     6961 aagtattttt cgagttgctt ttcttcggaa agtttagtta catccttttt gctatcttca
     7021 agatttttat atgctttttc tgcaaactct cccaaaacct tattttcggt cttgtattca
     7081 atgttgtttc cgttactttt tgccgttatt ccccgaacaa aatcttcata cgagtaagca
     7141 ggatgaaatt gaattaattt aaaatttcca gaatcttcca gttcttttat ttttttcttt
     7201 ctctcacctt cgttctctgg taagatatca tctttgatta atttgtatgc aatttctttt
     7261 gcaactctag tctttcctgt tcctggaggt ccttggagga taatttgatg tttgtgttcg
     7321 aggagtttag tgtacgcatt cataggccac cttttaaaaa aaatttcctg aattgcttca
     7381 ccatcagtta cttcaattag tgtatccggc catcttcctg ttctaggtcg acctgataat
     7441 tcaaattttt caattcccca tgatttaaaa ctgatacttt ttttataatc atctttggaa
     7501 attacttcac ctatgtaatg aatttttatt ttctgtggac taaatccttt aatcgcaaac
     7561 caatctcctt ttttaatctt gtcaaagttc tcaaaagcac tttttgctgt tgaagactga
     7621 tcaggggagt catatttcaa tgcagcccaa tagttgttta aaataaaatc atttaatacc
     7681 tccggttcac cgaaaccgcc tgcccaatat tttttttctg ccataaaaac tccttcttaa
     7741 aataaaaata aatacactag cttctacatt ttgttttaca gtaacattct acaagaactt
     7801 ttcaaaccta tatctcgggc taaagttaaa accctgattg tgtatgtttt tataatcttc
     7861 aatccaaacg taaattagtt ccataatata catgcaagag ttgtaaggac aaaaaagaga
     7921 gaactatttc ctagacttgc aaacttttat tgtccttcca attttaaaaa ataagtgaga
     7981 cgttattcgt atcctaaaat agaaatctct tacttaatac tatctaccaa tttttgattg
     8041 atgaagtcga gtcttggcat gtagcccatt tctttgcaac tgagttggtc aaagatttcg
     8101 gaaacaacgt attcatatcc agttggattc aattgcgttc ctgaacgata gccgctcaag
     8161 atgagtttat cattgaatat tttaaagtag aattcttgca tttctcttgc tcctgggggt
     8221 tgcagaagaa ttttaacgtt tttatctttc ttatttcgtt tcatgtctag attttgtttt
     8281 tctattttac ttcctgtatt tggattttga ccaaggtata gcataaacaa atcttttaat
     8341 tctaatccag aatagttttc ctcacctgtt ggattgtaaa tcttatatgc aagcacatcc
     8401 tttgtgtctg gaaattcttt tcctagatac acaagtaatt ttctatctaa aacagtttga
     8461 attgcttctc tgcttgcctt tctttcataa tttgtagatg cttttcctac attttcgatt
     8521 aattttttaa tcggttctgt ctttaatttt tctagctttt ggtctatcgc atctatttcc
     8581 tcttgcgtgt gttctggttt tgtttctttc ttcggagttt ctgtttcttc tgcaggagcg
     8641 ggtgctctct ctaatgtgct cgaacatcca attataaata ataagctaat tatcagtaaa
     8701 tacttcattc tttcttcctt tgttaatcca tttcgctcat catacaacat tgccttctta
     8761 ggattcggaa actttttttc cgattcttct ttttttaaaa aattttataa aatgatagaa
     8821 taacttattt tcaaaaaaag aagttgcatt cgagatagag attcctatga aagaacaatg
     8881 aagaacaaaa aaaatcttac agcgagtgag caattgattt tggcgattga gtttttaagt
     8941 ctttcggaag aatatattat tcgaaataaa aaattaaatt cgattcattt agcagaattt
     9001 ttagatgaac tccaagatcc agagcgagaa cgaaatgata cagaatggag tccggatcga
     9061 aaatttagat tgtatctaac taagttacgg aatatcggca ttagcctcaa tttaaaaacg
     9121 aaaacaattc aatttgataa gaatgtaaat ctttttaaaa ttctttctat ttatgtaaaa
     9181 tatttttgtg aagactatac cgaacaacat cttcgtttat tttataaatc taaaaaagaa
     9241 aatttctttg agtctttgcg gctattcgtt tttattcgct atgccattca atattctctt
     9301 ccgattgagt tatcgttttt aaagactatg gactggaaac cttctttcag gcgagtaatt
     9361 ccaagatacc ttgcttccaa aaatcaattg ctagatgtag ttgtaactga tttgaaggac
     9421 aatgttaata aatggttttc tcttgtaaat attctctcga ttgaaaatga tttgtattct
     9481 gcttttagaa aaaggaacaa aagaaatctt ccctttgatc gcgcttcttt tgaatcttct
     9541 ccagaaggaa gatttcattg ggcgaatatc gtttatacag ttcagtttaa tagttatact
     9601 tttaatcatt gcataaacac aaacgaactc gattataaaa taattaaaaa cgaaagtcct
     9661 ttgcaaatga tcgtagaaat tacctgcaat gattcttact taatccaaag aattcttttt
     9721 aattacggag tctatgtaaa acttctcgca cctagcgaag ctgtttcaca attcaaaaat
     9781 aaactttcag cccttgagaa gcactatcgg taaatttcat tttcataaac taaaacaggt
     9841 catttggaga tttcttctaa agccgaattc attatcacaa attattagaa ttcaactttg
     9901 gaacattcct attcgtaact aagtgccttc taaaaaataa gctttgattt tttaactcta
     9961 ggcagataat tatcatggga tcagttattc tgtatagaga agagcaatat aaaaaagtct
    10021 tgctttatga atttagtaaa ttaaattatg aggtaagagt ttaccatgcc gtctcagata
    10081 aaaaattttt ataccatcga ggagattaaa aaaaatctct ctgaatattt ttatgataaa
    10141 cctgttcgca aagcctatat ttttggttct tatgctaggg gagaagcgaa tgaattaagt
    10201 gacatggata ttcttgtaga attagatttt actgaaccaa taggtcttta tttttttact
    10261 atggaatatg aattgaaaaa aatctttaat caaaaaattg atttaataac gatagaagaa
    10321 atctcaccct ttataaaacc ctacatagat aaagataaaa ttttagttta tgaaagaaaa
    10381 gaataaaata acagatcgtg ttcgaatttc aattgtgcta gaagcgatac aagatattga
    10441 atcatttatg cattctattt cacaagaaaa ttttctttca aatcgattgg tgcaaagtgc
    10501 ttgtgcaaag aaactacaag atttatgtaa tgcatctgaa gatatttcag atgaaattaa
    10561 aatcaaaaat ccagatattc cttggtctca gatggatgga attcgaaata gaatcgctca
    10621 cgaatatttt ggaataaact ataaaattat ttggacagta attcaaaaag atcttccgga
    10681 aataaaaata aattttgaaa agattctgcc ttctttagaa gattgattcg agaacatttc
    10741 tattgattta aataaattga accaatagaa aacaatattt cacgacctat caaacgttaa
    10801 attcatactc gcatagaact gtattttcaa ttggaaaaaa gagtaggcta ggggctttga
    10861 ttttaaattg atcgagtagg attttgagtt gtcctttgat ttggactttg ccttcttttt
    10921 cgacggtagt tgcaatgctt tctagggatt gtgtgactcc attgattttt gcatctaggt
    10981 agattttgta ttcttctttt ggttttattt cttctacttt tgtgatttga agttcgattg
    11041 attcttgttc gggataacct aaaacttcta gcatgtgtga gtcgcgattt ggatcacctg
    11101 ttctcatatc tagtaatttg aattgaatcg agaaaggttg cgccgaatat ctgttagcct
    11161 cttttttgag agaaagattg ttgattgaaa atttttgaac cttgccattt acagttttaa
    11221 atttatgctt cactgtaaaa ccgattttat cctgtgtagt ttcacttgat aaaattccaa
    11281 aactaagtaa aaaaagaatg attagtctaa tcatgattat cctctgttta aaaataaaat
    11341 gttgcaatcg aaagacttaa agcaccgaat cccacccatc caatagtttg catttcattg
    11401 attacatgtg gtcctccttt ttgcacttgg ctaccaagag aagcaagtgc cagcatagaa
    11461 ggcaaatgca cgaagatcgc tgctttgtgt gtaaaaatgg tagaccaacc ttcttctcta
    11521 tctgcttgca ttttagaagg tgagaaaaag gcgagtccag ccgttatgct atacattaaa
    11581 aatgtagctc ctgctaaact tccatgtgta gcaccacctt ttgaatcata gggggaaact
    11641 tgcatgattc cataataaaa aagtgcgttc tgacttggat ttgcaaggag cacaacgttt
    11701 gcaatgggtt catattcttt tttataagta tttgctgctt tttccccagc aatgtttgtt
    11761 gcaagccaaa gtccccaagt tgcaaatcca gtgatctggt gccattctag aaaagatctt
    11821 ctacgctcga tttgtttttt atttaagaca ggactggatt ctggttttgg attcatagca
    11881 tttgattcta ccaaaaaaga attctgttgc ggaaaattaa agttaattga accaccatcc
    11941 caatagctaa cagggcgaat gtttccaaaa agatttgtat aattgggatt caaattgtca
    12001 taaaaggatt ctgcaggtag agtaaggaag gaaaaaagaa aaaggataaa taagattaat
    12061 ttcgaataca taattgccct ttaaataaaa ttctacttca atcctttttt acatacttat
    12121 tctgtatatt gaattttttt gcttctttga atgatttgta tgattcatta taaaaatatt
    12181 tatatttttc tttctggaat ttttttttgg aatggaattt cttttgttta gtttttcaat
    12241 ctatcaattt cgactaacga taaatggtgt tagatggaat ggattctaga actttttttt
    12301 attgaatttt tagaaatctg caaatattta caatgcaagt tgttgtattc gcttgagttg
    12361 gttctgaaaa tattgacaag ataagttgtt gtatttactt gaattgattt tgaaaatatt
    12421 gacttaaata ttcattatag gaaaagattc ttttatgctc acaaaaagag aaagacagta
    12481 cctagaacat aaactaaaat acaataaagc tctgttgttt atgcttcttt ctatcttccc
    12541 actagtattt cttcctatga tcttagattt gcaaatattt cggaatctat taagctttaa
    12601 aacaatttct ttttttctca ttcttttctt tttagtggga gcattttatg atgttttcac
    12661 tgcaaagatt cattggaatg atagaaatat aattgaagag attggaaaaa taaaagtaat
    12721 agaaggggag caggtttcta tattttggag cgatcatgtt ttgacttttc ccaagacttg
    12781 ggatataaat catatcaaga ctctatttgc aaaggatgta aaaattaaat actatcactt
    12841 gaaagtattt ccttctttta aaaaattcaa attcgcaatc aactgtgttt taagtctaga
    12901 tgagttttca gttgaagaag acattaaaaa agaaaaagat tggagagatt cattctctca
    12961 gctctttcac ctaccgttcc ttattttgtt tattggattt gggattatga gttctgaagt
    13021 gaaagaagat ttggatgcta gattttctaa agaacaaaat attgagatcg aaaaagttac
    13081 tcaactttct atggtatctg caagtatcca agaaatatta aatgaaaaat atccatttcc
    13141 agtatttgta aaattagaaa aaattctatt tatcgaggaa aattttatcc attggattat
    13201 ccccatcagc gaagacgatc ttattcaatt gagaaaagac tttaccgaac tagaagaaag
    13261 acaaaatcta attcagtcta ttttagataa aaatttaaat gatattaaaa aaataaaaaa
    13321 acaggatttg aaggtccttg aaaataatcc atttctgagt gattttttgg aaactcttag
    13381 aaaatacgat agtagaacta taaaactatt atttgaaata gatttaaatc aaaaagactt
    13441 aagaagtttt ccattgttat ctgaaacaca aaaggaaaat atgattgaga tacaaaaaaa
    13501 gggaatttta tcattttcta attttgaagt tgattttttt aaaactaaga tgcaaacatc
    13561 ttatgatttt ataatctcca aaaaacaaaa aattgcaaat gattattttc tttctcagtc
    13621 tacttggtca gttccacaaa taaatcgctc tgatctattt gcgatggatc aaacaaaatc
    13681 ttcactctgg aaaaaattct acacagaaag tttagaaaat aaaaattcaa tccaatgcta
    13741 tatttttaag aaagaaggaa gatatattct cattccgaaa gacttctctc aaaatgattt
    13801 aggcgatttt ttatatctga aacgagaata tgcatttttc ctaaattcta gttttttatt
    13861 tttaatttta gaatctattc cagttttcct atttctattg gatcacttta aacggaggat
    13921 agcgccttct ttaaaaatca aaaactactg aaatttactc attttcgata tatctttaga
    13981 atgcttggaa atgtagtttt tataaaagca gagaaagggt tttcattaca ctgatcccct
    14041 tttttggtaa ctttcgtact actcattcag tttattatca aaattgtatt ttctattttc
    14101 gaaacttgct ttctttccct catcaactcg gctattagaa aaaagattca atctttatag
    14161 cgatccccta atcaaacgat ggaaggatct gaatccattc aatcgtcttt ggtgcaaaaa
    14221 tttggaaaaa tagtgtcatt cctgtaacga ttcaggagag taatttatga aacacactca
    14281 tttctcattg cgagaaacaa tctttactat tgaactcacg ttttttaatc aacttgctaa
    14341 attttattga catgaaagtc ttttatcaaa accattcagt tagacggagg tttagcagtg
    14401 ggtagtgcac cagaaaattt aggaccaatt atcattcaac ttttgttagg aataggcttt
    14461 gccgccttga ttctctctct cgcttttctt ctcaatccca aaaaacgtag taaaccacat
    14521 gacacattcg aatgcggtgt tccttattac ggagatgcgc gcggtgtttt taatattaag
    14581 ttttatttag tagccgtttt atttatctta tttgatatcg aggctgtctt tttgtatcct
    14641 tgggctgtga atcttggtaa attcacagaa gccggtctag gcggattcat tttaattgag
    14701 atgcttttat ttatttttat cttggtagtc ggtctatact atatctggaa aaaaggagct
    14761 ttagaatggg attaaatgaa attctaagta agcccgggca aatgctcggc gatatggcac
    14821 aggttgcaaa tttagatgcg gtagttaatt ggggcaagag ttattcgctt tggccgtatc
    14881 catttgctac tgcttgttgc ggtattgaat ttatgggaac tgcttgttcc gataatgaca
    14941 ttgctcgttt tggagcagaa agaccttctt tctccccacg tcaatccgat atgattttag
    15001 ttcttggaac gattacctat aaaatggctc ctgttctgcg tgaaatctat gatcaacttt
    15061 gtgaaccaaa gtatgtaatt agtgtaggtg cctgtgcttc ctctggcggt atgtttgata
    15121 cctatggagt agtgcaaggt gttgatcgat ttcttccagt agacatttat gttccaggat
    15181 gtcctcctcg tccggaagct attatggatg cgttagtaaa actccaaaag aaagtccaaa
    15241 cacaaggtct cgaagctaga cgccaagagg tcatgagaaa gatcgatgag atcaatgaaa
    15301 aaaataaacc aatgattgta agatgaaaga aaaagtagaa tcgtttttaa aatccaaatt
    15361 agcttctttt attttcaaag aagaagcagt gaacagcaat atccccacat tctttattaa
    15421 gtctgagggg atttttcctg ttattaaggc actcaaagaa gacaaagagt taaagtttga
    15481 ttatctaaat gacttaactg ctgtcgattg gttaggcaag aagactcctc gttttgaggt
    15541 ttgctatttg ctcagatctc tttcgaataa acatacccgt attatgttaa aagttcccat
    15601 agaagaagga gagacgattc catctatcgt tcctattttt aaaggtgcga attggccaga
    15661 aagagaagtg ttcgatatgt ttggtattga atttactgga caccctcaaa tggagagaat
    15721 cttactacct gataattttc aaggtcatcc ccttcgtaaa gactatccac tcgaaggttt
    15781 tggtcaagat tatctaatcg cagacttatt acaaatccac caagaagaca aggaggctaa
    15841 ttaactatgg caatgtatga taaaacagcc cagaaattta aaggaaagtt tgataactta
    15901 ccagaagggc atctccttgt aaatttagga ccttctcacc ctgcaacaca tgggattttg
    15961 caaaacgtga ttcaaatcga tggagagcgt attgtagaag cggaagcaat tataggctac
    16021 gttcacagat gctttgaaaa actgggcgag agatacgatt acaatcag
//
LOCUS       DAOQOS010000120        16034 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_1841_length_16034_cov_5.687402, whole genome shotgun sequence.
ACCESSION   DAOQOS010000120 DAOQOS010000000
VERSION     DAOQOS010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 16034)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16034)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16034
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1841_length_16034_cov_5.687402"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1054
                     /locus_tag="PLF66_20340"
     CDS             <1..1054
                     /locus_tag="PLF66_20340"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HMZ66451.1"
                     /translation="NAKIKSELNYCSIIIWQENNNDSIKFEDIKQRFPSALIFVIVEE
                     LSEFIIDGINDKKFFKVVKNNFDPALLVRFIEEGFSFWNEQRKNEKQNKNIEKETLKK
                     ELEIAQEIQKNLLPNDSIKWKNLELECFSESAKNVGGDFYTYYGMQNDRALISKHIVA
                     VGDVSGKGISAALIMATAISHIDNSMQMNLKLSDRAVFLDKLLTPLTKPQRQNCAVCL
                     VEFVGVNTNRAFIKVVNAAFIPPYLKRKNGEVEWIASKGFALGQGLGMQFGYKEMTVG
                     IEKGDIIVLVSDGIIEANNEINELLGFERFYSILEEAPNDSAKKMLSYIQKKISQYTN
                     DSDQNDDRTIVVIRFH"
     gene            1311..2663
                     /locus_tag="PLF66_20345"
     CDS             1311..2663
                     /locus_tag="PLF66_20345"
                     /inference="COORDINATES: protein motif:HMM:NF021597.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2330 domain-containing protein"
                     /protein_id="HMZ66452.1"
                     /translation="MNSKLKWLAISGILLLSVTPFKKILAFCGFYVAKADASLYNKAS
                     RVAVVRNQDRTVISMMNDFQGDLKDFAIVVPVPVILEKDQIHVGDSKIMDHLDAYSAP
                     RLVEYFDPDPCYVYEEEKSLMDVFRKKEMNRPVPSSNTAGKKDLGVTVEASYTIGEYD
                     IQILSAKYSDGLETWLNQNGYKIPKGASEALKPYIQQKLKFFVAKVNLKSIAQGLTFL
                     RPLQFAFESPKFMLPIRLGMLNAKGEQDLLLYMLSKEGRVETTNYRTVKLPSDLEVPE
                     FVKSDFANFYKAMFTEQARKENHRVVFTEYFWNMSWCDPCAAEPLSNQELEQLGVFWL
                     GDNGKQKQNWGGAPQVMITRLHLRYNNDTFPEDLVFQETKDQTNFQGRYIIQHPWKGS
                     PNQCSQAKEYFKQLRVREEQRAENYRTLTGLDIKEIRGRMKLQEEPKEEDKWWKKIWK
                     "
     gene            2838..3353
                     /locus_tag="PLF66_20350"
     CDS             2838..3353
                     /locus_tag="PLF66_20350"
                     /inference="COORDINATES: protein motif:HMM:NF019235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1564 family protein"
                     /protein_id="HMZ66453.1"
                     /translation="MTTSNLFSNQKMYFPRNLTLSDFSSTLLIPDPFFNLFQSKIKKH
                     KGIHLYISYLLKKYKLYIANGLVPAYSNVTTKYQERGQNLHKIAFRPKPDDWAELKLY
                     RISFGMSISAFLVYLLKADSVDFAEASSEYLTSVGIIVVPDFDLCTKVLLHSDRSTYT
                     IIFQYRRGKYH"
     gene            complement(3443..4636)
                     /locus_tag="PLF66_20355"
     CDS             complement(3443..4636)
                     /locus_tag="PLF66_20355"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HMZ66454.1"
                     /translation="MQYKKPFFIILISWTLASFGFATILPFLSIFLSDQYGLSVSEIG
                     IFFLLTAILRSIFQMYSGEISDLFGRKNIMSYSQIARAFLLILAVIAIYFKLSYLYVV
                     SLVGISYVFASMFQPVAQAALADHLPIENFLQGFSLIRVAGNFAWSLGPIIAGYVTKI
                     SYSALFISSAVFSILGAILTHRHYIDATKLDKKENRTNLGTVFNDKLFLTFCLITFFL
                     CITISQMFSSLSIYLSKYKHLDSETIGYIFAINGFTVLIFQMPISKLVTSKIKPLYGM
                     SIGSLLYMLGYFIAGYANSLLGYILILFCVSLGEVFVTPLIQTSVAKLAPEKMIGRYM
                     GFYSMIAVAGWSFGPYIGGKILDFFSLNFHIAWQMISLFALFASIGYFLLDKILVKTG
                     NVREL"
     gene            4866..5852
                     /locus_tag="PLF66_20360"
     CDS             4866..5852
                     /locus_tag="PLF66_20360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66455.1"
                     /translation="MKLVLVFQVYLLYISLPMQLFSDVGDLDYTFSREKYLTIENSFR
                     VKMSGLEFVIPTQIGPRILHFSRIGEENIFKVMEDQIQNSKSKSWLNFGGHRLWRAPE
                     DKILTYSPENSNVEIQTQKEFISISKVDSVNQILKEIEISPIKDYSIQVKHKITNLSD
                     KKQTLSVWALTVLPLSGKALIPLSPRGTHPQDLLPTDSVQLWAYTNLSDSIFNFKKNY
                     ILIEKKENSHLPQKIGLNGEPGWAAYINGANVFIKKFSYKKYRNYPDKNSSIEVFVNQ
                     KFLELETLSPIRTLKPKESITHIETWILEKVPNSISTDDQLFDFIQTKIQEK"
     gene            complement(5893..6969)
                     /locus_tag="PLF66_20365"
     CDS             complement(5893..6969)
                     /locus_tag="PLF66_20365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66456.1"
                     /translation="MQTVSIEYIQEFKKDNSISLRGEKFKQMKSSVDEFKKRFKQRGQ
                     VEAVRTIDLISAAYPLSFAYAGAAIGINPYVNIVNRLVLVKYKDFNGKSRILAWEPTI
                     REGSANSPFYRQLLDFYGEFLSYKILTTQYNTLFTALAKAGLTSDQIDYVTFDHLHVQ
                     DLRYLMGTTKPIQGEAENRKPFFPNAKFIFQKKEMDTLRSPHPMQWAWYVTECTENLD
                     EKNLILIDGDVELGYGIALIATPGHTDGNHSLCINTPDGIWVSSENGVSLDSWFPEYS
                     KIPGIRSYSKFYNREVILNSNTLEDSIEQYDSMVKEKLLADVNRKYPKLKNIQPSSEV
                     GRFYRHFPVIPTFQMGGMNYGKLI"
     gene            complement(6987..8219)
                     /locus_tag="PLF66_20370"
     CDS             complement(6987..8219)
                     /locus_tag="PLF66_20370"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HMZ66457.1"
                     /translation="MNWLLKKTEKEILPILEEENSKNARQIAIFRIIASSLWLLLCII
                     IGIGFDRTYWKVEIPWLIGYVLLSVLILRGAKTNSRLHWIIHWSTVIADIPIVYLIMK
                     TSLESAPYPQAGAMYTLCIFLLFLLPAPSGVFHLPTIIAGFETIGFTILLLNQSGVTF
                     PTWVGSVILISVFSVFVAINVSRRVIGVAGQYAQEKTIRNQLARYFSPSIAQEILEIK
                     NKNTNPVVSVLFADIRGFTAYSESVPSERVVAFLNEYLTLMVEIIFKHGGTLDKFIGD
                     GIMAYFGAPIEQEDHSKRAILCGLEMLSKMKTFNEKRKLENEKEMQIGIGIHSGKVVL
                     GDIGPEQRKEFTIIGDTVNLASRIESLTKEIGMPILVSEESMILSKKDFNWTKTNPIK
                     VKGKEKEIIVYSPLLNFD"
     gene            8804..9583
                     /locus_tag="PLF66_20375"
     CDS             8804..9583
                     /locus_tag="PLF66_20375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66458.1"
                     /translation="MKIFRIVLVLCVLNTFIFAQEETINKEEPTDAKTTAANASIEKM
                     SQDIKDYFKEEKAASLYYLGAGGLTTAMGLYYRREAVDYYPSPKGVKQDNAYYLGLSY
                     PLLGVGVFQLATGGFMYFKSDKRAKDLEKELEANPASFREQELSRIENVAYWNKIFRY
                     AQYGMIGGGLWAWYAGNLSDKDYMKGFGQALFLQGVISFALDYFTNKRTDAYREKLIN
                     FNFSYIPPTRNINSYAVNGTGVNGPVLNEGYYMFTLSYILK"
     gene            9729..11486
                     /locus_tag="PLF66_20380"
     CDS             9729..11486
                     /locus_tag="PLF66_20380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016752995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HMZ66459.1"
                     /translation="MNRKDLTIDRVFEINSRINSSHDLNSLLDTIMETIKDVLNTEGC
                     SLLLYLKEEDCLYFHTSKGEKSDLLPSMKVPRGRGIAGLVLETLEPVIVNDAQQDDRI
                     YREIDKSVGFVTKNLICVPMVAQGEVQGVVEAVNTVDRDSFEEYDIHLLQNLSIMAAI
                     AIRNRILVEDLESKYNEIQCLLKVSQALQNINNIEQFLEVAFRSALELINVERFSFAY
                     KSRSKGNWRLVKTYGFTLDPEYIYIDASHGVIGHIIRTGEPLLVTNTDETKLEFLYPE
                     NYKSKSFISIPIFLNSEIFGILSVSDKKNKKAFNSSDLHLLIIISNHILEAYKALLSK
                     EKEKKLEAIHRDLQIASKIQLYSLPVIPKKLKGMQVESFYQSSREIGGDFYDLIYHGE
                     NEFSALIADVSGKGIPAALFMEFSKTILASEVHSIKSPAKSLHNANQILKEKFNYMML
                     VEVMLLRIFTNEKKFIFASAGHNRQFYYKKSSGKVNLLSGKGIPLGTRMQNFEISETT
                     VSYNSGDLIILYTDGITETMNHNKEMFGEERFIELIENNAELPLESLKDLIKLKTDEF
                     RGRSENLEDDCTLLLIRLE"
     gene            complement(11666..12175)
                     /locus_tag="PLF66_20385"
     CDS             complement(11666..12175)
                     /locus_tag="PLF66_20385"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HMZ66460.1"
                     /translation="MKDAYIAMSREQGIRMYELILESKAKNIIEFGTSFGISTLYLGA
                     GAKENGGKVITTELLEEKAKIAKQNFKEAKLDDYIELREGDAMQTLQDSRENIEFVLL
                     DGWNDLYLPLLKMLEPKFKDGCIIITDNVNFSSANSFLEYVRNHKHYKSSYLDPNDNR
                     TEKTIYKKQ"
     gene            complement(12277..12411)
                     /locus_tag="PLF66_20390"
     CDS             complement(12277..12411)
                     /locus_tag="PLF66_20390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66461.1"
                     /translation="MKNKIILILTLSVFIHCFQKERTLNPIYTNTELNTNKELKQTKN
                     "
     gene            complement(12645..13298)
                     /locus_tag="PLF66_20395"
     CDS             complement(12645..13298)
                     /locus_tag="PLF66_20395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66462.1"
                     /translation="MQRFLGTIIILMSIVMSCKGERRSLQTVDEIRNFIKGNKWISIT
                     TELRPQEDRTGTGKVQPFYITRTFEFFEGDKFTGTITSFGDPYQKFPLVKFEFKGHVE
                     WQGEHPVAKGAQKIDYVLDEGFIVTPLNPNFADALNQFPVTGLNKFEVNVSQDILKKP
                     FPLFNIKEGQIVKDYDLIYIYNDMLFMGAKHVDGTPFDKPENRPHLLQIPLIKKESK"
     gene            13394..13732
                     /locus_tag="PLF66_20400"
     CDS             13394..13732
                     /locus_tag="PLF66_20400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002080223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HMZ66463.1"
                     /translation="MNEKKIKISDESCPVTYCMKKIGGKWKPVVLYLIRKGANRFGIL
                     QRGIEGISKQMLTTQLRELEEDGILERIVYAEIPPRVEYRITKYGKSLFPIIDSMSKW
                     GNKYRNYMVE"
     gene            13829..14422
                     /locus_tag="PLF66_20405"
     CDS             13829..14422
                     /locus_tag="PLF66_20405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66464.1"
                     /translation="MKSFILLFLFIFSFFYSCKKTDSRLDQIHQIQSNQTLTEEESLY
                     QMARIIFSDTLKNFKLEKNSRGANDVSIEFGGNRAYTFYSESDYKEKIQFDVVRYILL
                     YSQVTENKNIGAIRISLVKPYFVKEPDAKKEITEEFEVFRVNISMEDIKKISGWNNPK
                     IWNDSRNINSSNMTELFNQVRLKWKIELNELSRIELK"
     gene            14427..14867
                     /locus_tag="PLF66_20410"
     CDS             14427..14867
                     /locus_tag="PLF66_20410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004788925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding domain-containing protein"
                     /protein_id="HMZ66465.1"
                     /translation="MKRKIIISLVVVFILLQLIPINRNNPKVEADIQAPESIKSILKK
                     SCYDCHSNETKYPLYSYIFPASVFLAHHVEEGREELNFSNWETLPANKKVKKLKEIKE
                     EIEEKEMPLSSYTIIHREALLSKEEMNQIIEWADQMIQKNESKE"
     gene            14851..>16034
                     /locus_tag="PLF66_20415"
     CDS             14851..>16034
                     /locus_tag="PLF66_20415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004750573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HMZ66466.1"
                     /translation="MNQKNEILSFRKQISSDPSWSSWKFQLQNRIKSESLEKYFLLSD
                     EERKAIQDSVRLNVGATPYYALLADPFDTNCPIRKTIIPQSGETLLSPEESPDPLHEE
                     RLSPVKGLTRMYDDRVLLFSNQECSVYCRHCMRGRKVSYNEERMEKEDLENAFRYLKD
                     HPEISDVVISGGDPLNLSDTRIDFILENLEKIDSIKICRIGSRNLVTLPMRITDDLCK
                     IIESHNTDRLSIFCNTHFNHEKECTKEAKEAILKLIKTGVSVGNQCVILKGINDSGEQ
                     MLALHKKLLEMRVRAYYMYDPEIIPGSRGIRTPLAKGFEIISYMRGKISGMGIPHFVN
                     DLPGGGGKITMTPKWYHGFHRETRQHLFKSALRGTYHLSPEPVNSEYDLLYEEVNPSL
                     WE"
BASE COUNT         5593 a         2483 c         2758 g         5200 t
ORIGIN      
        1 gaatgctaaa ataaaatcag aattgaatta ttgttctata attatctggc aagaaaataa
       61 taacgactct attaaatttg aagatataaa gcaaagattt ccctccgctc ttatttttgt
      121 tatcgttgag gagctttcag aatttattat tgatgggatc aatgacaaaa aattttttaa
      181 agtagtgaag aacaattttg atccagcttt actggtaagg tttatagaag aaggattttc
      241 tttttggaat gaacaacgaa aaaatgaaaa acaaaacaaa aatattgaga aagaaacttt
      301 aaaaaaagaa ttagaaatag cccaagaaat tcaaaaaaat cttttaccaa atgattcaat
      361 aaaatggaaa aatctggaat tagaatgttt tagtgaatca gctaagaatg tgggtggtga
      421 tttttatacc tattatggga tgcagaacga tagagctttg attagtaagc atattgttgc
      481 agtaggtgat gtgtctggga aaggaatttc agctgctctt ataatggcta cagctatttc
      541 gcatatcgac aattctatgc agatgaatct aaaactgtct gatagagcgg tttttttaga
      601 taaattattg actccgctta caaagcctca aaggcaaaac tgtgcagtat gcctagttga
      661 atttgttgga gtgaatacaa atagggcatt tataaaagtt gtaaatgcgg cttttattcc
      721 accttattta aaaagaaaaa atggagaagt agaatggatt gcttctaagg gatttgcatt
      781 agggcagggt cttgggatgc agtttggtta caaagaaatg actgtaggaa ttgaaaaagg
      841 tgatataatc gttttagtga gtgacggtat tattgaagcc aacaatgaaa taaatgaact
      901 cttaggtttc gaacggtttt attctatctt agaagaagct ccgaatgatt ctgctaagaa
      961 aatgctttct tatattcaaa aaaaaatttc acagtataca aatgatagtg atcagaatga
     1021 tgatagaacc attgtagtaa ttcgttttca ttaaataagg ttacaatgcc aatgtaaatt
     1081 cacataatca ttatttgtcg aaagatagat tcacatagaa tacatacatt tagtgttttt
     1141 ggtttgaaaa gaaattaatc atgacaattt gaaaattagt attagagtta tttgtatttc
     1201 tctagagaat tgaaaatgtt tttagaaaat atgattgaaa gaataaaaaa attggatcta
     1261 tttaggaaat gtaaatcatt ctgagatgat tcaaacaaga gaggtttgaa atgaattcaa
     1321 aattaaaatg gctagcaatt agtggtatat tgctattatc cgttactcca tttaaaaaaa
     1381 tattagcctt ttgtggattt tatgtagcaa aagccgatgc tagcctttat aataaagctt
     1441 cccgtgtggc agtggttcgc aatcaagata gaacagtcat tagtatgatg aatgattttc
     1501 aaggggattt aaaagacttt gcaattgtag ttcctgttcc cgtaatttta gaaaaagatc
     1561 agattcatgt tggggatagt aaaattatgg atcatttaga tgcatatagc gctcctagac
     1621 ttgtagaata ctttgatcca gatccatgct acgtctatga agaagaaaag agcttaatgg
     1681 atgtctttcg caaaaaggaa atgaatagac ccgtcccaag ttctaataca gcgggaaaaa
     1741 aagatttagg tgttaccgtt gaagcttcct atacgatagg agaatacgat attcaaattt
     1801 tatcagctaa atactcagat ggtcttgaga catggctcaa tcaaaatggc tataaaattc
     1861 caaagggagc aagcgaggcg ttaaaaccat acattcaaca gaagttaaaa ttctttgtgg
     1921 cgaaagtaaa cttgaaaagc attgcacaag gtctaacgtt tttacgacca cttcaatttg
     1981 cattcgaatc tcctaagttt atgctcccaa tcagactcgg aatgctcaat gctaaaggag
     2041 agcaagattt acttctctat atgctatcga aagagggtag agttgaaact acaaactata
     2101 gaaccgttaa actaccaagc gatctagaag tccccgaatt tgtcaaatct gattttgcaa
     2161 acttctacaa agcaatgttt acagaacaag cacgcaaaga aaatcataga gttgttttta
     2221 ctgaatactt ctggaatatg tcttggtgtg acccttgtgc agcagaacct ctttctaacc
     2281 aagaattgga gcaactcggt gttttttggc taggggataa tggcaaacaa aagcagaatt
     2341 ggggcggagc acctcaggtg atgattacta gacttcactt gcgttacaat aatgatactt
     2401 ttccagaaga tttagtcttt caggaaacca aagaccagac gaattttcaa ggtagataca
     2461 tcattcaaca tccttggaag gggagtccaa atcagtgctc tcaagcaaaa gaatatttta
     2521 aacagttaag agttagagaa gaacaaagag ctgagaacta tcgcactctc actggtctag
     2581 atataaaaga aattcgtgga agaatgaaat tacaagaaga acccaaagaa gaggataaat
     2641 ggtggaagaa aatttggaag taaccagaaa tccttatccc cctctttcca ttgacaactg
     2701 aaagataata aatctgtgct ctcataccga aaggttctga gagctcataa aaaaacaatt
     2761 tgcataaaaa aagttataaa aattacgcac atgatttcgg aaaaatttct ctatttcaat
     2821 agaaaggaga aatttctatg acaacttcta atttattttc gaatcagaaa atgtattttc
     2881 caagaaatct gactctctca gattttagtt ctactcttct catacctgat ccattcttta
     2941 atttgtttca aagcaaaata aaaaaacaca aaggcattca tctttatatt tcctatctgt
     3001 tgaagaaata taaactttat attgcaaatg gtcttgttcc tgcctactca aatgtcacta
     3061 caaagtatca agaaagaggt caaaatttgc acaagatagc ctttcgtcct aaaccagatg
     3121 attgggcaga actcaaattg tatcggatta gttttggtat gtctatctca gcctttttag
     3181 tttacctact aaaagcagac tctgtagatt ttgcagaagc ctcttcggaa tacctaacca
     3241 gcgtcggaat tatagtggtt cccgattttg acttgtgcac aaaggtctta cttcatagcg
     3301 atcgatcaac ttatacgatc atttttcaat atcgaagagg caaataccat taaaagtaaa
     3361 gaaaaaattt ttacttagca gctaatacag tataacctca agaacggcaa tggcggagct
     3421 atacaaacag taaaaatagg aattaaagtt ctcgaacgtt tccagttttc acaagaattt
     3481 tatctaaaag aaaataacca atgcttgcaa agagggcgaa gagtgagatc atttgccagg
     3541 cgatatgaaa gttcaaggaa aagaaatcta aaatttttcc accaatatac ggaccgaaag
     3601 accagccagc gactgcaatc atagaataaa aacccatata tctaccgatc attttttctg
     3661 gagccagttt tgcaacactg gtttggatca atggagtgac gaaaacttcg ccaagagaaa
     3721 cgcagaataa aattagaatg tagcctaaca aagagtttgc gtagcctgct atgaagtaac
     3781 ctaacatata aagtaaggaa ccgatagaca ttccatacag tggtttaatt ttgcttgtta
     3841 ccaattttga tattggcatt tgaaaaataa gaacagtgaa tccattgatg gcaaagatgt
     3901 atccaattgt ttcagagtcg aggtgtttgt atttacttag gtaaatagac agagaagaga
     3961 acatttggga aattgtaatg cataaaaaaa acgttatgag gcagaaggtc aagaatagtt
     4021 tatcattaaa aacagttcct agattagttc tattttcttt tttatctaat ttagttgcat
     4081 ctatataatg tctgtgtgtg agaattgctc cgagtatact aaaaacagca gacgaaataa
     4141 acaaagcaga ataggaaatt tttgtaacat atccggcaat aattggtcca agactccatg
     4201 caaaattacc agcaactcga atcaagctaa agccttgtaa gaaattttct attggtagat
     4261 gatctgcgag agcggcctgt gcaacaggtt gaaacataga tgcaaaaaca taacttatgc
     4321 caactaacga aacaacataa agataggata atttaaaata aattgctatg acagccaaaa
     4381 ttaaaagaaa agctctggcg atttgtgaat aggacattat attttttctg ccaaataaat
     4441 cagaaatttc tccgctatac atttgaaaaa tggatctcaa tatagctgtc aataggaaga
     4501 agatacctat ttctgaaacc gatagtccat attggtcgct caaaaaaata gatagaaaag
     4561 gcagaattgt tgcgaaaccg aatgaagcaa gagtccagct gatcaagatt atgaaaaaag
     4621 gttttttata ctgcatataa tgaaaataga ttgatttctt aatttagcgg ataataaata
     4681 ttaaccacaa ttttctattc tgtgtaaaag gtttttatcg ccatagcaaa atagatctct
     4741 caaactactt gacaattaac aaaattagcc taccttatta tgagagataa tgtcagatta
     4801 aaaaaaggat aaaaatgatc aaaagtatta tatttaaaaa tttttccaat aaaaacatag
     4861 ctaaaatgaa attagtttta gtttttcaag tttatctcct atatatatca ctgcctatgc
     4921 aacttttttc agatgtaggt gatttggatt atactttctc ccgagaaaag tatctgacta
     4981 ttgaaaattc tttcagagtg aaaatgagtg gtttagaatt cgtaataccg acccaaattg
     5041 gaccgaggat tttgcatttt tccagaatag gggaagaaaa catttttaaa gtaatggaag
     5101 atcaaattca aaatagcaaa agtaagtctt ggcttaattt tgggggacat cgactttgga
     5161 gggcgcctga ggataagata ctaacgtatt ctccggaaaa ttcaaatgtt gaaatacaaa
     5221 cgcaaaaaga attcataagt atttctaaag tcgattcagt gaatcaaatt ttaaaggaaa
     5281 ttgaaatttc tcctataaaa gattattcta tccaagtcaa acataagatc acaaatcttt
     5341 cggataaaaa acaaacactt tcggtctggg cgcttacagt cttgcctctg tcaggaaaag
     5401 cacttattcc attatctccc agaggaacac acccacaaga tttacttcct acagattcgg
     5461 ttcaactttg ggcatatact aatttatcag attcaatatt taatttcaaa aagaattata
     5521 tactaattga aaagaaggaa aatagccatt tacctcaaaa gataggatta aatggggaac
     5581 caggttgggc agcttacata aatggagcga atgtatttat aaaaaaattt tcctataaaa
     5641 aatatagaaa ttatccagat aaaaattcta gtatagaagt ctttgtaaat caaaagtttt
     5701 tagaattaga aactttgagt ccaatcagaa ccttaaaacc aaaagagtcc attactcata
     5761 ttgaaacatg gattctagaa aaagttccaa attctatttc tactgacgat caattgtttg
     5821 attttattca gactaagatt caagagaagt agacttgcaa tgtattacct ttcctttacc
     5881 tagggtcatt tattaaatta actttccata attcatccca cccatttgaa aagtaggtat
     5941 cactggaaaa tgtctgtaga atcttcctac ttcagaggat ggctgtatat tttttaattt
     6001 tggatatttt ctatttacat cagcaagaag tttctctttt accattgaat catattgctc
     6061 aattgagtct tctaacgtat ttgaatttaa tataacttca cgattataaa atttggaata
     6121 agaacgaatt cctggaattt tagaatattc tggaaaccat gaatcaagtg agactccatt
     6181 ttctgatgaa acccatatcc catcaggagt gttgatacag agagaatggt ttccatcggt
     6241 gtgtcctggt gttgcaatta acgcaatacc ataacctagt tctacatctc cgtcgattaa
     6301 aattagattt ttttcatcta gattctctgt gcattcagta acataccaag cccattgcat
     6361 tggatgaggg gagcgcagtg tatccatttc ttttttctga aaaataaatt ttgcgtttgg
     6421 aaaaaaaggt tttctatttt ctgcttctcc ttgtatcggt tttgtagttc ccatgagata
     6481 acgtaaatct tgaacatgca gatgatcaaa agttacatag tcaatttgat cggaagtaag
     6541 acctgctttt gcaagtgcgg taaacaatgt gttatactgg gtagttaaaa ttttatacga
     6601 taaaaactct ccataaaaat ctaatagttg tctatagaaa ggggaattag cagagccttc
     6661 tcgaatcgtt ggttcccaag caagtatcct actttttcca ttaaaatctt tatatttaac
     6721 aagcaccaaa cgattcacaa tatttacata tgggttgatt ccaatcgctg cacctgcata
     6781 ggcaaaagag agaggatagg cggcagagat tagatcgata gttctcacag cttcaacctg
     6841 tcctctttgt ttgaatcttt ttttaaattc atctacggag gatttcatct gtttaaattt
     6901 ttcaccccga agtgaaattg aattgtcttt tttaaattct tggatgtatt ctattgacac
     6961 agtttgcatt tttatttcct tttgaatcaa tcaaaattta aaagaggaga gtaaacaata
     7021 atctcctttt cttttccctt cacctttata ggattcgtct ttgtccaatt aaaatctttt
     7081 tttgatagaa tcatagattc ttctgataca agaattggca ttccaatttc ttttgtaaga
     7141 gattcaattc tagaagcaag attgactgta tctcctatga ttgtaaattc ttttctctgt
     7201 tcaggtccta tatctccgag aactaccttt ccggaatgaa tgccaattcc gatttgcatt
     7261 tctttttcgt tctccaattt tctcttctca ttaaatgttt tcattttaga taacatttct
     7321 aatccacaaa gaatggctct cttgctatga tcttcttgtt ctattggtgc tccaaaataa
     7381 gccatgattc catctccaat gaatttatcc aatgtacctc catgtttaaa aatgatttca
     7441 accattagag tcaagtattc attgagaaaa gctacaactc tttcacttgg gactgattct
     7501 gagtaagcgg taaatcctct aatatcagca aacaaaacag acaccactgg atttgtattt
     7561 ttatttttaa tttctagaat ctcttgtgcg atgctaggtg aaaaatacct ggcaagttgg
     7621 tttcgaattg ttttctcttg cgcatattgt ccggcaaccc caatcactct tcgcgatacg
     7681 ttaattgcta caaaaacaga gaatacacta attaaaatga ctgatcctac ccaagtaggg
     7741 aaggtaactc cggactgatt gagaagtaat attgtaaatc caattgtttc aaaaccagcg
     7801 atgattgtag gcaagtggaa aactccagaa ggcgcgggaa gtaaaaaaag taaaaatata
     7861 caaagagtat acatcgcccc tgcttgcgga tatggtgcgg attctagact tgttttcatg
     7921 atcagatata caattggaat atctgcgata actgtagacc agtgaatgat ccaatgaagt
     7981 cttgaatttg tcttcgctcc tctgagaatt agaacggata atagaacgta accaattaac
     8041 catggaattt caaccttcca ataggttcgg tcgaagccaa tcccaatgat aatacaaagt
     8101 aggagccata gggaggatgc aataatgcgg aaaatagcaa tttgcctcgc attttttgaa
     8161 ttttcttcct ctaagatagg tagtatttct ttttctgtct tttttaataa ccagttcaat
     8221 ttttaatttt ccagaagatt ttacaatctt aatccataat accataataa ctaattttca
     8281 aaaaagtcat ctggtcaatc ctttaaatgg atatggaaaa atcttctatg attgaaaagc
     8341 ctttttattc aattcgattt tatctttaaa ctggaaggaa ttttgaacat ctagatagtt
     8401 tttcttagaa ataatcctta tttcacactt ttaaagagga tatttctcta tgtgtctgac
     8461 ttctctgtag ggtgaatgtt tttttgccga ctaaggtttt acttccgaat tcctttgacg
     8521 gattatgaat tagaaatggt agagcactca atttagttga aaggtataaa tttttatttt
     8581 ttttgggatt ttcgttgaaa ttttaaagcc taataattct gatttcctag agaaaaagaa
     8641 aaactaattt tcaaaaataa aaaatttacc tgaataaata attctaccga gaattttagc
     8701 agtagacagc aaagaccgaa tttacaatgt gtttatagaa acacttttaa atcctgtatt
     8761 tgaaacaagt aaccgcaggt gaatataatt aggagtcgtt agaatgaaaa ttttcagaat
     8821 tgttttagtt ttgtgtgttc taaatacatt catttttgca caagaggaaa caataaataa
     8881 agaagaacct actgatgcaa aaacaaccgc agccaatgca agcattgaga aaatgtccca
     8941 agatataaag gattatttca aagaagaaaa ggctgcttct ttatattatt tgggagcagg
     9001 tggtcttacc actgctatgg ggttgtatta tagaagagaa gctgtggatt actatccaag
     9061 cccaaagggt gttaaacaag ataatgcata ttatctaggt ttatcttatc cacttttagg
     9121 cgttggtgtt ttccaattag ctaccggcgg ctttatgtat tttaaaagtg ataaaagagc
     9181 aaaagacctt gaaaaagaac tagaagcaaa tcctgcttct tttagagagc aagagctttc
     9241 ccgtattgag aatgttgctt attggaataa aatatttaga tatgcacaat atggaatgat
     9301 tgggggagga ctttgggctt ggtatgcagg taatttaagt gataaagact atatgaaagg
     9361 ttttgggcaa gctctctttt tacaaggggt tatatccttt gcactcgatt atttcaccaa
     9421 taaaagaaca gatgcgtatc gcgaaaagtt aattaacttc aactttagct atataccacc
     9481 tacaagaaat attaactcct atgccgtcaa tggaactggt gtcaatggac ccgttttaaa
     9541 tgaaggctat tatatgttca ctctaagtta tattttgaaa taacacaccg attcaattct
     9601 attaaaccag attgccgaaa ggcgtctggt tttttcttac ttaaacattc attcatttta
     9661 ctctagttac catttttaat ctttcgaaaa atatttgtaa ataatccaca taagtccatt
     9721 gtatggctat gaatcgaaaa gatctcacta ttgatagagt tttcgaaatt aattccagaa
     9781 ttaattcctc tcatgatcta aattccctcc tagatacgat catggaaacc attaaagacg
     9841 ttttaaatac agaaggctgt tcactcttgc tttatttaaa agaagaagat tgcctctatt
     9901 ttcatacaag caagggagag aaaagtgatt tgctaccttc tatgaaagtt ccacgaggac
     9961 gagggattgc tggtttggtt ttggaaactc tagaacctgt tattgtaaat gatgcccaac
    10021 aggatgatag gatttataga gagattgata aatcggttgg ctttgttact aaaaacctca
    10081 tctgcgttcc aatggttgcg cagggggaag tccaaggagt tgttgaggct gtcaatactg
    10141 ttgatcgaga cagcttcgaa gagtatgata tacatttact ccaaaatcta tccattatgg
    10201 cggctatcgc aattcgaaat agaattttag tagaggattt agagagtaaa tacaatgaaa
    10261 ttcaatgtct actaaaagta agtcaagcct tacaaaacat aaataatatt gaacaatttc
    10321 ttgaagttgc ttttaggtct gcacttgaat taatcaatgt ggaaaggttt tcatttgctt
    10381 ataaaagtag atcaaaagga aattggagac tcgttaagac ttatgggttt acattggatc
    10441 ctgaatatat atatattgat gcgagtcatg gcgtaattgg tcatatcata cgaacaggtg
    10501 aaccattact tgtgacaaat acggatgaga ctaaattaga atttttatat cctgaaaatt
    10561 ataagagtaa atcttttatc tctataccta tttttttaaa ttctgaaatt tttggaatct
    10621 taagtgtttc cgataagaag aataaaaaag catttaatag ttctgatcta catttactta
    10681 tcattatttc taatcatatt ttagaagcat ataaagcgtt actctccaaa gagaaagaaa
    10741 aaaaattaga ggcaatacat cgagatcttc aaattgcttc aaagatacag ttgtattcat
    10801 taccagtcat tcctaaaaaa ttaaaaggta tgcaggtgga atctttttat cagtcttcta
    10861 gagagatagg aggcgacttt tatgatttga tttaccatgg agagaatgaa ttttcagcac
    10921 tcatagcaga tgtatctggt aaaggaattc cggctgcatt gtttatggaa ttttcaaaaa
    10981 ctattttggc tagtgaggtg catagcataa aatctcctgc caagagttta cacaatgcga
    11041 accaaatttt gaaagaaaaa tttaattata tgatgcttgt ggaagtcatg cttcttcgca
    11101 tttttactaa tgagaagaaa tttatttttg caagtgctgg tcataatcgc caattttatt
    11161 ataaaaaatc ctcaggcaaa gtaaatctac tcagtggaaa aggaattcca cttggaacta
    11221 gaatgcagaa ttttgaaatt tcagaaacga cagtttccta taattcaggg gatttaatta
    11281 ttttgtatac tgatggtatt acagaaacaa tgaaccataa caaagaaatg tttggggaag
    11341 aaagattcat tgagttgata gagaataatg ctgaattgcc ccttgaaagt ctaaaagatc
    11401 taattaaatt aaaaacagac gaattcagag gaaggtctga aaacttagaa gatgattgca
    11461 ctcttttact gattcgatta gaatgattta ttaactcacc ttacgcaaaa ttgacatttc
    11521 aagaaaaaag aaatgatttt aataatatta ggaaatgcta ataaactaat aaaatcaata
    11581 gcctgcagcg gcaagccctc tgctaggagc aaacctcatt tgaacgtttg gctcaccttg
    11641 cgtaaggtga gttattaaaa gtaaatcatt gttttttata aattgttttt tccgttctat
    11701 tatcattagg atctaaatag cttgatttat aatgtttatg gtttcttaca tattctaaaa
    11761 aagaatttgc acttgaaaaa tttacgttat ccgtaataat aatacagcca tctttaaatt
    11821 taggctctaa catttttaaa agaggtaagt ataaatcgtt ccagccatct aataatacaa
    11881 attctatatt ttctcgagaa tcttgaagtg tttgcattgc atcgccttcc cgcaattcta
    11941 tataatcatc gagttttgct tctttaaaat tttgttttgc tatttttgct ttctcttcta
    12001 gtaactctgt tgtaattact tttcctccat tttcttttgc tcctgcacct aaatataagg
    12061 ttgaaatgcc aaatgacgtt ccaaattcta ttatattctt tgctttactt tctagtatta
    12121 attcatacat tctaattcct tgttctctgc tcattgcaat gtatgcatct ttcatatctt
    12181 ttggttctaa ttttctaaag attcccttcg agagaccttt taaaatagtg aaaatttcac
    12241 catcagattc tttgtgtaac cgatttagtg tttgttttaa ttctttgttt gttttaattc
    12301 tttgtttgta tttaattctg tgtttgtata aattggattc aaagttcgct ccttttgaaa
    12361 acagtgaatg aagacggaca aagttaagat tagtataatt ttattcttca taaatccttt
    12421 cattttttat ataccctaaa ccataagtag tttcccattt ttgaaaaagt tggctacaca
    12481 caattgccaa agataaagtt cagttaaaaa ccaacgggaa atttctttgg gcaattggta
    12541 taattcaatt ctaagaataa atgaaatgaa tctttactct tagaatacaa tttttcgttt
    12601 tatattaaaa ttctctaatt caataaaaac ttgatttaac gaatttattt actttctttt
    12661 ttgataagtg gaatttgtag aagatgcggt ctattctcgg gtttatcaaa aggagttcca
    12721 tcaacatgtt ttgcacccat aaacagcata tcgttgtaaa tatagattag atcatagtct
    12781 tttacaattt gaccttcctt tatattaaat aatggaaaag gttttttaag aatatcttgt
    12841 gagacattca cttcaaattt attcaagcct gtaactggaa attgattgag tgcatctgca
    12901 aaatttggat tgagtggagt aacaatgaat ccttcatcta atacataatc aattttctgc
    12961 gcaccttttg caacaggatg ttccccttgc cattctacat gtcctttaaa ttcaaacttc
    13021 acgagtggaa acttttgata tggatctcca aaacttgtga ttgtgcctgt gaatttatct
    13081 ccttcaaaaa actcaaaagt tctagtgata taaaaaggtt gaacttttcc tgttccagtc
    13141 ctatcttcct gtggtcgaag ttccgttgta atgctaatcc atttatttcc ttttatgaaa
    13201 tttctgattt catctacagt ttgtaagctc cttctttcac ccttgcaact cattacaata
    13261 ctcattagaa ttataattgt tcctaaaaat ctttgcattg aatactccta aaataattta
    13321 taggttgtat ttaagcatat aggattgaaa atgtcaacta gtcatgtttt taagactaat
    13381 gggaaattat actatgaatg aaaaaaaaat taaaatatcg gatgaatctt gccctgttac
    13441 atactgcatg aaaaaaatcg gaggaaagtg gaagcctgtg gttctctacc tcataagaaa
    13501 aggagctaat cgatttggta ttttacaaag aggtattgaa ggtattagca aacagatgct
    13561 caccacacaa ttaagagaat tggaagaaga tggaatactc gaaagaattg tctatgcaga
    13621 aattcctcca cgggtagaat atcgaattac taaatatgga aaatccttgt ttccaatcat
    13681 agattctatg tctaaatggg gaaataaata tagaaattat atggtcgagt aaatattttt
    13741 aagttaaaat gaattggatg catatttgtc taggattctt ttttcataaa tctatttaca
    13801 aagtaaaatg gatttatact ttgctcttat gaaaagtttt attctactct ttttatttat
    13861 ttttagtttt ttctattctt gcaaaaaaac agattctcgt ttagatcaaa tacatcagat
    13921 tcaatcgaat caaacgctaa cagaggaaga aagtctctat caaatggcta gaatcatttt
    13981 ttctgataca ttaaaaaatt ttaaattaga aaaaaattca agaggagcaa atgatgtaag
    14041 tattgaattc ggaggaaata gagcttacac attctactca gagtctgatt ataaagaaaa
    14101 aattcaattt gacgttgtgc gatatatatt actttattct caggtaacag agaataaaaa
    14161 tataggagcg attcgtattt cgttagtaaa gccatatttt gtaaaagagc cagatgcaaa
    14221 aaaagaaatt acagaagaat ttgaagtctt tcgtgtaaat atttcaatgg aagatataaa
    14281 aaaaatttct ggatggaata atcccaaaat atggaatgat tcaagaaata ttaattctag
    14341 taatatgact gagcttttta atcaagtacg tttaaaatgg aaaatagagt tgaatgaact
    14401 aagtcgaata gaattaaagt aagagaatga aaagaaaaat aattatctcc ctagttgtag
    14461 tgtttattct tttacaacta attccaatta atagaaacaa tcctaaagta gaagcagaca
    14521 tacaggcacc agagagcatt aagtcgattt taaagaaatc ctgttatgat tgtcattcta
    14581 acgaaactaa gtatccactt tactcttata tatttcccgc ttctgtcttt ctagcacatc
    14641 atgtagaaga aggaagagaa gaactaaatt tttcaaactg ggaaacgtta cctgcaaaca
    14701 aaaaggttaa aaaattgaaa gagatcaaag aagaaattga agaaaaggaa atgcctcttt
    14761 cttcgtatac aattattcat agagaagcct tattatcaaa agaggagatg aatcaaatca
    14821 tagaatgggc ggatcaaatg attcaaaaaa atgaatcaaa agaatgaaat attaagtttc
    14881 aggaagcaaa tatcttctga tccaagttgg tcatcttgga agtttcagct tcaaaataga
    14941 attaaaagtg aatctttaga aaaatacttt ttactttctg atgaggaaag aaaagccatt
    15001 caagattctg ttcgcttaaa tgtaggggca actccttatt atgcgctcct cgcggatccc
    15061 tttgatacaa attgtccaat tcgaaagacc attattcctc aatctggaga gactctatta
    15121 tcaccggaag aatctccaga cccactccat gaagagagac tttcacctgt gaagggactc
    15181 accagaatgt atgatgatag agttttactt ttttctaatc aggaatgttc tgtctattgc
    15241 cgtcactgta tgcgcggtag aaaggtatct tataatgagg aacgtatgga aaaagaagat
    15301 ctcgaaaatg ctttccggta tttgaaagat catccagaaa tttcagatgt agttatttct
    15361 ggaggagacc cactgaattt aagcgatact agaatcgatt ttatattaga aaatttagaa
    15421 aagattgaca gcattaagat ctgcagaatt ggttctagaa atttagtaac tcttccaatg
    15481 cgcataacgg atgatttgtg taagattatt gaatcacata atactgatcg tctctcaatt
    15541 ttttgtaata ctcacttcaa tcatgaaaaa gaatgcacaa aagaggctaa agaagcaatt
    15601 ttaaagttaa ttaaaactgg agtcagcgtt ggaaatcaat gtgtaatttt gaaagggatt
    15661 aatgattctg gagaacagat gttggcatta cacaagaaac tacttgagat gcgtgtcaga
    15721 gcttattata tgtatgaccc agaaatcatt cctggttcgc gtgggattcg cactcctctt
    15781 gcaaaaggtt ttgagattat ttcctacatg agaggtaaaa tttcgggaat gggtatacct
    15841 cattttgtaa atgacttacc aggaggagga ggaaaaatta caatgacacc aaaatggtat
    15901 catggatttc acagagagac gagacaacat ctatttaagt ctgcgttacg tggaacatac
    15961 catttgagtc ctgagccagt aaactctgaa tacgatctgc tttatgaaga agtaaatcct
    16021 agcctttggg agaa
//
LOCUS       DAOQOS010000121        15824 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_1877_length_15824_cov_5.964487, whole genome shotgun sequence.
ACCESSION   DAOQOS010000121 DAOQOS010000000
VERSION     DAOQOS010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 15824)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15824)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15824
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1877_length_15824_cov_5.964487"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1109)
                     /locus_tag="PLF66_20420"
     CDS             complement(<1..>1109)
                     /locus_tag="PLF66_20420"
                     /inference="COORDINATES: protein motif:HMM:NF039196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HMZ66467.1"
                     /translation="NTIKNNLNNAIDNVNALVADAAMLSQAAIEGKLATRADATKHQG
                     DYRKIVEGVNQTLDAVIGPLNVAADYVDKISRGAIPTKITSTYNGDFNTLKNNLNRCI
                     ENVNLLVNDAGMLAKAAIDGKLATRADATKHEGDFRKIVDGVNNTLDAVILPVQEASN
                     VLLELSKGNLKSRVTGDYKGDHATIKNALNSSLVTLNEYISEITRVLTSMSAGDLTVE
                     IAKDFSGDFAEIKKALILIVDSFNELIGEIINASDQILISSRQVADSSQSLSQGSNEQ
                     ASSVEEITSTLAVIELKAKDNAKNAKDASDQALNVKDQAVASNNEMKGMLKAMEEISE
                     TSENIAKIIKEIDAIAFQTNILALNAAVEAARAGQ"
     assembly_gap    1110..1209
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1210..2786)
                     /locus_tag="PLF66_20425"
     CDS             complement(<1210..2786)
                     /locus_tag="PLF66_20425"
                     /inference="COORDINATES: protein motif:HMM:NF024140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCP four helix bundle domain-containing protein"
                     /protein_id="HMZ66468.1"
                     /translation="MFKNLSIKSMLVLSFSGVLLLFLGMSIYNLFALNEIQTSVTGVV
                     NDRIPKILILHEMNNDLNQTARSTRSMLLLEDPREIQKERDAILNVRDEKFPKVFDYL
                     KTVIRRERGKELLSEATRARDSYIESLNRFLGMYDSFQKEDAKKLLLTELRPRQHEFA
                     RSLKMLTDYQKDIVTTEGKKTEENVSQIYFLFFIISILGTLITILVTFVATQKINSTT
                     SRMDEFISDINIFVKELESKGDFAFRLKIAHKDEMGKTSEALNRLMNMFQGIQRETNL
                     LSRAAIEGKLNTRADLSGYIGDFRNIIEGVNKTLDAVIGPLNVAADYVDKISKGTIPK
                     KITDNYNGDFNEIKNNINSLIEATNMIADVAYKLSVGNTKVSLTKRSPDDTMIENLIK
                     VIENNKHDTGNVVKMAKGDFNFEVKVMSEDDEMAKSCVNLQTTLKTLIADMAGLSKAA
                     VEGKLATRADATKHQGDYRKIVEGVNQTLDAVIGPLNVAADYVDKISRGAIPAKITDT
                     YNGDFNTIKNNLNNAID"
     gene            complement(3298..3990)
                     /locus_tag="PLF66_20430"
     CDS             complement(3298..3990)
                     /locus_tag="PLF66_20430"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HMZ66469.1"
                     /translation="MSNILCIGAHPDDVELSMGGSIFRLKEAGHSVVLLDVTDGEPTP
                     HGSIEIRAEESTKAAELLEVKRITLDIKNRFIEETIENRIKLAEVFREVKPDYIFTHY
                     EYDVHPDHIAVNRLTDSARFYSKLTKCSIKGEPFFPKKVIYYFPNHIKLNLEPSFCVD
                     VSPFIEKKKQILECYESQFIKKGQGLMIREAIESNRYFGMRIQTEYAEPFYMRDSIGI
                     LEVGSLFLKNKI"
     gene            complement(3983..4918)
                     /locus_tag="PLF66_20435"
     CDS             complement(3983..4918)
                     /locus_tag="PLF66_20435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002756524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HMZ66470.1"
                     /translation="MPSKKKNQNKSQNTLNKKEIALAIAGGGCKALYGLGVGYKLRSW
                     GLKIKEISGVSAGSAIGLMILSRREEAGIEYFEQLLKRNKSNFWPHHLLMGKRPFPHE
                     NMYRRTLRYAIDFEKISKSRVKIFILAIKAFPRKGKLHNYWKKLLLIPKTMRAVLLDD
                     KDKERGIPCSRVDRIVEEWNLKEVIFTNKDFQYPSITEQIIMNSSSIPPVLSFQRINE
                     EYYLDGGLTNNLLLEPFSPNLKKIGIYYENTTLYGKTEDTLKNTFLIKPKDKLPIHTF
                     DYTNAIGAREAYEIGKKDAEEQKEKIFSFLNSDYE"
     gene            4998..5405
                     /locus_tag="PLF66_20440"
     CDS             4998..5405
                     /locus_tag="PLF66_20440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66471.1"
                     /translation="MIQTNKFLYIVTFTLLSGLFFSQSAHDSDKKSKSKDVPKENTDM
                     TLHDFMEDYVEAAEKKFKKGNKEPLKKILEFLPETAIPEDKEEWQKITEKYLASEEYL
                     ASCKACHSKFKKSYKKSYKKRLVTIPKEILNLK"
     gene            5405..7786
                     /locus_tag="PLF66_20445"
     CDS             5405..7786
                     /locus_tag="PLF66_20445"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HMZ66472.1"
                     /translation="MKEDILSKLSSLIQDKNVLDEVKKIILNEEDTNTGKFAQKEYLL
                     SHAKRIGNIATFDMNVETRKVHWSPEIYNILERPKEDIKGDANEYINCIHPEDVDAVN
                     AALATLLEDGKPFYIEYRLIMPDGRIKYVSSEGVLEVADDSSKRFVGLTQDITERIRR
                     EEELKIYRHILKNNWEAVVFADINGIVRYANYSAYELYGYNEPELIGQSVDIFNSHAT
                     FKTEQIIDSVLKNGGWSGELTQKKKDGTFIDTLLTISLVTNDQGQPIGYSSNSKDIAE
                     KKYYERKLLEVRNFLDSIINSIPTPVFVKDALHNWILLNDAFCGFIGLKREELLGKSD
                     YDFFSREEADSFWYNDNKVLIDGDEIITEESITDTRNIKKTILTHKRLYVDDSGDKYI
                     VGVITDITRNKEFENELIKAKEYAEVATLAKSEFLANMTHEIRTPMNAILGFSELLKN
                     EIKEGKARRYLEAIMSSGKNLLNLINDILDLSKIEAGKMELKNSPAMIHSILSELKEI
                     FSLKSAEKNIELNFIISGSFPKIILIDELRFRQILTNLIGNAIKFTESGSVTVKINSV
                     FNRNFPNKMDLLAIVEDTGIGITEKEQDKIFEAFYQQLDQNISKFGGTGLGLTISSKL
                     AEMMKGRIDLKSKVGEGTTFTVYIPDIQILNEDLKEQGNHPNLGFSKFSLLIADDVEY
                     NRKLIKDFLSEYEIDFYEAADGLRAVELARELKPDIIFMDIRMPFLDGIDASRIIKES
                     EELKHIPIIALTAFVMKGAEDQIKKYCEGFLRKPISKRDLLSELMTFLPTRIL"
     gene            8133..8798
                     /locus_tag="PLF66_20450"
     CDS             8133..8798
                     /locus_tag="PLF66_20450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66473.1"
                     /translation="MEFDEHFKHILQLYQDSPDIRILKLEYPIPNKYETAQEEFLEKF
                     FQNNPFEIFPNDLETFTIQNAKIKRLKFDRRPSTFIGILRFIELYRQQFNSIKKNLSL
                     LARRYKTFEKNDLLDPENSVLKNEIFTQILAAGRVLKKWTPQVELLYKNTLVNINTNL
                     AESELVNGKGKGFVNLRKIHNEGYLNQTFHPYYQNLLNFCIMCIAFATKLEENTKKWK
                     IAD"
     gene            9088..9276
                     /locus_tag="PLF66_20455"
     CDS             9088..9276
                     /locus_tag="PLF66_20455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66474.1"
                     /translation="MIFRKVILLQTKTARGNNAYILIFFSEIFSFASLLIHSIKNFMI
                     AFHNFRNHKKESGGLGSR"
     gene            9509..10669
                     /gene="metK"
                     /locus_tag="PLF66_20460"
     CDS             9509..10669
                     /gene="metK"
                     /locus_tag="PLF66_20460"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HMZ66475.1"
                     /translation="MSLNDFIFTSESVSEGHPDKVCDQISDAILDAFLAQDPKSRVAC
                     ETLVTTNLVVVAGEITSLGKIDAQEIARGVINKIGYNDISMGFDAEFAVVQAHIHKQS
                     PDISQGVTEGQGLFKEQGAGDQGLMFGFAIDETKELMPMPIQYSHALMKKLADLRHGK
                     KLKFLRPDAKSQVTVIYQNGKPVAIDTVVISTQHTPDVKHKQIKEAVIEECIKKVIPA
                     NLLKKTKYFINPTGNFVIGGPHGDTGLTGRKIIVDTYGGYGRHGGGAFSGKDPSKVDR
                     SAAYMGRYIAKNVVAAGLASKCEVQLAYAIGVAEPVSVLVDTFGTSKIPDAEIVKRVK
                     ANFKLTPRGIIETLGLLEKGRKYSDTASYGHFGRTGETFTWERTDKAEALKG"
     gene            10669..11640
                     /locus_tag="PLF66_20465"
     CDS             10669..11640
                     /locus_tag="PLF66_20465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020770684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HMZ66476.1"
                     /translation="MGAVTTNTSSELKATRDAYGDALAELGSENPSIVVLDADLSGST
                     KTNVFKKKFPDRFFNLGVAEQNLVGHAAGMALTGLIPFASSFAMFLAGRAWEIVRNSV
                     VYPNLNVKLVASHGGITLGEDGASHQIVEDFAIMRVIPGLTVICPSDYNETKLVIKKI
                     AEYKGPVYVRVGRPPIPLIHRENYQFEIGKGELLSEGKDVLIVACGLLVGESLKAIET
                     LKEKGISVSLINMATIKPIDKELILKHAKICKAVVTCEEHSVIGGLGSAVSELLGEEF
                     PVPVLKLGMNDVFGKSGTWSELLDYFGMRAKNIVSLAEKVIQMKGKN"
     gene            11900..12109
                     /locus_tag="PLF66_20470"
     CDS             11900..12109
                     /locus_tag="PLF66_20470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015678642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HMZ66477.1"
                     /translation="MDPEIQTEKIVTQNKTFLVDLKKNQAGYYLKVSEWSNSKKSSIF
                     VPAEGIDKLIEILSRFKTLIANDKE"
     gene            12172..12930
                     /locus_tag="PLF66_20475"
     CDS             12172..12930
                     /locus_tag="PLF66_20475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011670758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament outer layer protein FlaA"
                     /protein_id="HMZ66478.1"
                     /translation="MKFKNIIMVVLMAMLGLPSLSAQEAKAAKSTESSGGGAAIEPLE
                     RVTLEDFEESEDWRAKSTTPLGQTKVLKMIQRGIIKDVFDEKSVPDKKNEKGEYVPVA
                     EDQKDRIGTNHILGVKTYFAEKGFDRVELSPPHEFIIKGKARQISVWVLGRKFNHTMY
                     AKFRDYKGNTHNIRLGRLDFFGWRKLTAPIPGYVPQSTRYSLLDKNIHFVSLFVTSDV
                     HEAAGDFYFYVDDLEVRSDKYDMIYPGFEIKDNW"
     gene            12956..13957
                     /locus_tag="PLF66_20480"
     CDS             12956..13957
                     /locus_tag="PLF66_20480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002975280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament outer layer protein FlaA"
                     /protein_id="HMZ66479.1"
                     /translation="MNNKIKYLSITSAVLAAVVAFSGIAFAENKGLKTTNGNDLGSNE
                     LKSITLETWDKTEWEVFTDKDSEIPDSYGKKPYKKDLPPSSQAMRQVKLLKGYPRDVK
                     YIDLSKDKENSQVLAIKFHFTFPGNNEVTIRPPRTADFLINRPRMFINENAFSSEEAK
                     KNPEKRDTNNVNVQPIYGVELPGVTKSMSVWVLGRGIDYNLEGWFEDYKGDTHIIKFG
                     SVNFVGWRPMTAVIPANIPQFVEAYPATKSLVFKQFKLRSTPKTGGDVTYLFFDELKI
                     LTDTFDVHFDGANLDFDPEDCSNKQRVEEVEAKAKGIKPVKDCGNPGSKQAENPAKK"
     gene            14643..>15824
                     /locus_tag="PLF66_20485"
     CDS             14643..>15824
                     /locus_tag="PLF66_20485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004479233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreAB"
                     /protein_id="HMZ66480.1"
                     /translation="MSQETVITNEKSSSELDKLTSLFNEEIYVRVDASSVPVTKFKIL
                     DDLIAHYKSIGKIEEANQKVEEHLKDHPESISARYLIGFISLIQNKIEDSNHLKSLLE
                     QFKIHGKWTIIEYISDHILQFGEQRLALKFKAEALEKLNKNKELKLVLEKLAKHDRKN
                     PEIAKKYALSILDEDRDKAIIFLKQAAESFARSKEYGQLEEIWPILIQHNFEDIPFFE
                     KIERILLSAREKSRIVILFYPLMETYKQLEDHDKTILLLKKILDHEPLSQKARNDLIR
                     AYKSKYANHSLLEEFLKMSEIGNSKKSIKACITNFERNIVFDTNNYVMHRNWGVGQIK
                     SISSSSDSIVVDFLGKKDHKLSIQMAITSLKPLKQDHIWVKLYENKQQIVDLFNNDIP
                     NF"
BASE COUNT         5485 a         2640 c         2571 g         5028 t
ORIGIN      
        1 ctgtcctgct cgcgctgctt ctaccgctgc gtttaacgca agtatattgg tttggaatgc
       61 gatcgcatcg atctctttga taatcttcgc tatgttctct gatgtctcac tgatctcttc
      121 catcgccttt agcattccct tcatctcgtt gttacttgct acagcttggt cttttacatt
      181 caacgcttgg tcacttgcat ctttcgcgtt cttcgcattg tcttttgcct ttagctcaat
      241 cactgctaag gttgaggtga tctcttctac gctacttgct tgctcattcg atccttgcga
      301 taacgactgg cttgaatcgg ctacttgtct tgaactgatt aagatttgat cagatgcatt
      361 gataatttct cctatcaact cattgaacga atctactatc agaatcaatg ccttcttgat
      421 ctctgcaaaa tctccactaa aatccttcgc tatctctact gtcaaatctc ccgctgacat
      481 tgaagttaat actcgtgtta tctcactgat atattcattg agtgttacta aacttgaatt
      541 caaggcattc ttgatcgttg catgatctcc cttgtaatca ccagtcactc tagacttcaa
      601 attccctttc gatagttcta gtaacacatt cgatgcttcc tgcacaggta gaatcactgc
      661 gtctagtgta ttatttactc catctactat ctttctaaaa tctccctcgt gcttcgttgc
      721 gtctgctctc gtcgctagct ttccatcgat tgctgctttt gctagcattc ccgcatcgtt
      781 tacaagtaag ttcacatttt caatacaacg atttaagttg ttctttaatg tattgaaatc
      841 gccattgtag gttgaagtaa tctttgttgg aatcgctcct cttgagattt tatctacata
      901 atcagctgct acattcaacg gtccaatcac cgcatcgagg gtttggttta ctccctcaac
      961 aatcttacga tagtcgcctt ggtgtttagt agcatctgct ctggttgcaa gctttccttc
     1021 gattgctgct tgcgataaca ttgctgcatc tgccacaagt gcatttacgt tgtcgattgc
     1081 attgtttaag ttattcttaa ttgtattgan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnt tgtcgattgc attgtttaag ttattcttga ttgtattgaa atcgccattg
     1261 taggtatctg tgatctttgc tggaatcgct cctcttgaga tcttatctac ataatctgcg
     1321 gctacgttta atggtccaat cactgcatcg agggtttggt tgactccctc tacaatctta
     1381 cgatagtcgc cttggtgttt cgttgcatct gctcttgttg caagttttcc ttctacggct
     1441 gccttggata atccagccat atcagcaatc aatgttttta gcgttgtttg taaattcaca
     1501 caactctttg ccatttcatc gtcttctgac ataaccttca cttcaaagtt aaaatctcct
     1561 tttgccattt tcacaacgtt tcctgtatca tgcttattat tctcgatgac tttgattaaa
     1621 ttttcgatca tcgtatcgtc aggagagcgt ttagtcaaag agacctttgt atttccaacg
     1681 gacaatttat aggcaacatc tgcgatcata ttggttgcct ctatcagaga gtttatatta
     1741 tttttaatct cattaaaatc tccgttgtaa ttatctgtaa tcttcttagg aattgttccc
     1801 tttgagattt tatcaacata atcagctgca acattcaatg gtccaatcac tgcatcgagt
     1861 gttttattta ccccttcaat tatatttcta aaatctccaa tgtatccact tagatctgct
     1921 ctcgtattca atttaccttc tattgctgca cgagagagta gatttgtttc tctctgaatt
     1981 ccctgaaaca tattcatcaa acgatttaga gcttctgacg tttttcccat ttcgtcctta
     2041 tgagcaattt tcagtcgaaa tgcaaaatct cctttggact ctagctcttt cacaaaaata
     2101 tttatatcag aaataaattc atccatccta gaagtggtag aattaatttt ttgcgttgca
     2161 acaaaggtaa caagaatagt aatcaaagtt cctaaaatag aaataataaa aaataaaaaa
     2221 tagatttggg aaacattttc ctcggtcttt tttccttctg tcgtaacaat gtctttttga
     2281 taatcagtca gcattttcag agaccttgcg aattcatgtt gtcttggtct taactctgtt
     2341 aaaagtaatt tcttagcatc ttccttttgg aaagaatcat acattcctaa gaatcggttt
     2401 aaagattcaa tgtaactatc tctcgctcta gttgcctcgg ataatagctc tttgcctctc
     2461 tctcttcgaa tcactgtttt taaataatca aatacttttg gaaacttttc atcgcgaaca
     2521 ttaagaatag catctctttc tttttgaatt tctcttggat cttcgagcaa tagcatactt
     2581 ctagtagaac gtgcggtttg attgagatca ttattcattt catgcaaaat taaaatttta
     2641 gggattctat catttacaac tccagtaaca gaggtttgaa tttcattcag cgcaaataga
     2701 ttgtaaatag acattcccaa aaataataaa agcacgccag aaaagctaag aactagcatt
     2761 gatttaattg ataaattttt aaacatatat caccctcctg aattttttat tttttcgctt
     2821 ttctattttt taatgaatcc aacaggagtt tgatgggaat agaattgatt gctctcaccc
     2881 aacaaagagt attgttcttt gcaaaaaaag ggtgaaagaa ttagatcatt cactaaaatc
     2941 cttgtattgt gttcatattt atcctaaact tttttcgatt ttaaagtagt gtttacacta
     3001 acgtcattct gttaccgttt ctgaaataga attaaaagat ttcaatccta agctttaaaa
     3061 acaccacatc cgatataact agaaaagttt cagaaactat ttatattatt attagaagaa
     3121 aaaaattatc aaatgctttt taaaaaaatc atctagagag aaaagtagat accagcccct
     3181 tagatttcga gaatgattct tagaagaaaa ctaatttttc atcatcactt cttttccttg
     3241 tatctaattt gattcactta gattatttaa acagaaggtt tcttatagaa tcagctctca
     3301 aattttattt tttaaaaaca atgatccaac ttctagaatc cctatggaat ctcgcatata
     3361 aaaaggttcc gcgtattcgg tttgaattcg cattccaaaa taacgattgg attctattgc
     3421 ctctcgaatc atgagtccct gtcccttttt tatgaattga gattcataac attctaaaat
     3481 ttgttttttc ttttcaataa aaggagaaac atctacacaa aaagaaggtt ctaaatttaa
     3541 cttaatatga tttggaaagt aataaattac ttttttagga aaaaaaggtt ctccctttat
     3601 actacactta gttaacttgg aatagaagcg agctgaatcc gtcagcctat tgactgctat
     3661 atgatctgga tgaacgtcat attcataatg agtaaaaata taatctggtt ttacttctct
     3721 aaaaacttcc gctaacttaa ttctattttc gattgtctct tctataaatc gatttttgat
     3781 atccaaagta attcttttaa cttctaagag ttcggctgct ttcgtgcttt cttccgctct
     3841 tatttctata ctgccatgag gagttggttc tccgtctgtt acatctaaaa gaactactga
     3901 atgacctgct tcttttaatc taaaaatgga acctcccata gaaagttcta catcatctgg
     3961 atgagctcca atacaaagaa tattactcat aatctgaatt taaaaaagaa aaaatctttt
     4021 ctttttgttc ctccgcatcc ttttttccaa tttcatatgc ttctctagca cctatcgcat
     4081 ttgtataatc aaaagtatga ataggaagtt tatcttttgg ttttatcaaa aaagtatttt
     4141 tcaaagtgtc ttccgttttt ccatagagag ttgtattctc ataataaatt ccaatctttt
     4201 ttagatttgg cgaaaacggt tctaatagta aattatttgt aagtccccca tccaaataat
     4261 actcttcatt aattctttga aaacttagaa caggaggaat cgaagaagaa ttcattataa
     4321 tttgctctgt aatcgatgga tattgaaaat ccttatttgt aaatatcact tcttttaaat
     4381 tccactcttc aacgattcta tccacacgac tacaaggtat tcctctttcc ttatctttat
     4441 catctaataa tacagctctc atagtttttg gaatcaaaag aagttttttc caataattgt
     4501 gcaacttgcc ttttctagga aacgctttta tcgcaagtat aaaaattttc actctagact
     4561 tactaatttt ttcaaagtca atcgcatagc gtaaggttct acgatacata ttctcatgcg
     4621 gaaaaggtcg cttacccata agtaaatgat ggggccaaaa attactttta tttcgcttta
     4681 ataattgttc aaagtattca attccagctt cttctcttct cgaaagtatc atcagaccta
     4741 ttgcagatcc agcactcact cctgaaattt ctttgatctt tagtccccaa cttctcaatt
     4801 tatatccaac gcccaaaccg taaagagcct tacagccacc gccagcaatt gcaagagcaa
     4861 tctctttttt atttaaagta ttttgagatt tattctgatt cttttttttt gaaggcataa
     4921 aaatagtagt tttactctat ttttttatcg caataaaaaa aagttttaaa aaaataaata
     4981 gcctgtaaaa tagtagaatg attcaaacaa acaaatttct atacatcgta acatttacac
     5041 ttttaagtgg gctttttttt tcccagtctg cacatgattc agataaaaaa tctaaatcaa
     5101 aggatgttcc aaaagaaaac acagacatga ctcttcatga ttttatggaa gattatgtag
     5161 aagcagcaga gaaaaaattt aaaaaaggaa acaaagaacc attaaaaaaa atcttagaat
     5221 ttctaccaga aacagctata cctgaagaca aggaagaatg gcaaaaaata actgagaaat
     5281 atctagcctc tgaagaatat ctagcctctt gtaaagcttg tcattctaag tttaaaaaat
     5341 cctataagaa atcttataag aaaagactcg tcacaatacc taaggaaatt ttgaacctca
     5401 agtaatgaaa gaagatattt tatctaaact ttcaagcctc atccaagaca aaaatgtttt
     5461 ggatgaggta aaaaaaatta ttctaaacga agaggataca aatacaggaa agtttgcaca
     5521 aaaagaatac cttctatctc atgcaaaaag aattggaaac attgcaacat tcgacatgaa
     5581 tgtagaaaca cgcaaggttc attggtcccc agaaatttac aatatcctag aacgccctaa
     5641 ggaagatatt aaaggtgatg caaatgaata tattaattgc atacacccag aagatgtgga
     5701 tgcggttaat gctgcattag ccacgcttct agaagatgga aaaccatttt atattgaata
     5761 tagactgata atgcctgatg gcagaattaa atatgtatcc agtgagggag ttcttgaggt
     5821 tgctgatgac tcttctaaaa gatttgtggg tctaacacaa gatattactg aacgtatacg
     5881 aagagaagaa gagttaaaaa tatatagaca tattttaaaa aacaattggg aagcagtcgt
     5941 ttttgctgat attaatggaa tcgtgcgtta tgcgaattat tcagcctacg aattatatgg
     6001 ctataatgag ccagagttaa ttggtcaaag tgttgatatc tttaattctc atgctacatt
     6061 taaaacagag caaatcattg attccgtctt aaaaaatggc ggctggtcag gagagcttac
     6121 tcagaaaaaa aaagatggaa cctttataga tacactttta acaatatctt tagtaactaa
     6181 cgatcaagga cagccaattg gttattcatc caacagcaaa gatattgcag aaaaaaaata
     6241 ctatgaaaga aaactcttag aagttagaaa tttcttagat agtattatca attctattcc
     6301 aacacctgta tttgtaaagg atgctctgca caattggatt ttgttaaatg atgcattttg
     6361 tggtttcata ggattaaaaa gagaagaact attaggtaaa tctgattacg attttttctc
     6421 aagagaagaa gctgatagtt tttggtataa tgataataaa gttctaatag atggggacga
     6481 aatcataaca gaagaatcca ttacagatac aaggaatata aagaaaacaa ttcttactca
     6541 caaaagatta tatgtagatg atagtggaga caaatatatt gtaggtgtga taacagatat
     6601 tacccgcaat aaagaatttg aaaacgagct aatcaaagca aaagaatatg cggaagtagc
     6661 aacccttgca aaaagtgaat tcttggcaaa tatgacccat gaaatcagaa cacctatgaa
     6721 tgcgattctt ggtttttctg aactactaaa aaatgaaatc aaagaaggca aagcacgcag
     6781 atacctagaa gcgatcatga gtagcggaaa gaatctatta aatctaatca atgatatatt
     6841 agatctttct aaaatagaag caggcaagat ggaattaaaa aactctcctg caatgataca
     6901 cagcatccta agcgaattaa aagaaatttt tagtttaaaa tccgcagaga aaaacattga
     6961 acttaacttt atcatctcag gaagttttcc aaaaattatt ttaattgatg aactcagatt
     7021 tagacaaatt ttaaccaact tgatcggcaa tgcgattaaa ttcacagaat ctggttccgt
     7081 aacggttaaa atcaattctg tattcaatcg aaattttcca aacaaaatgg atttacttgc
     7141 gattgtagaa gatactggaa ttgggattac cgaaaaagag caagacaaga tatttgaagc
     7201 attctatcaa caactagatc agaatatcag taaattcggt ggaactggtc ttgggttgac
     7261 tatttcctca aagttagccg aaatgatgaa aggaagaatt gacctaaaaa gtaaagttgg
     7321 tgaaggcaca acgtttacag tttatatacc agacattcaa attttaaatg aagatttaaa
     7381 agaacaaggg aatcatccaa atttaggatt ctccaaattt tcacttctga ttgcggatga
     7441 cgttgaatac aatagaaaac taatcaaaga ctttctaagt gaatatgaaa ttgattttta
     7501 tgaagcagca gacggtctta gagctgtgga actagcacga gaactaaaac cagatattat
     7561 cttcatggat atacgaatgc catttttaga tggaatcgat gcaagtagaa ttataaaaga
     7621 atcagaagaa ttaaagcata tacctattat tgctctaact gcttttgtta tgaaaggagc
     7681 cgaagatcaa attaagaaat attgtgaggg tttcttgcgt aaaccaatta gcaaaagaga
     7741 tttactctct gaattaatga ctttcctacc aacgagaatt ctatagttgc aaattgcata
     7801 ttttaaaaaa cagaaaatat agaatcatga gaaagaaagt atttactctt tttttattta
     7861 tatacccttc ccattataga aagagttggg tattccaaat tgcaaaaaaa ggctcttttc
     7921 aaatatgaat tggatttttc ttttgttaat tggtataata actaattgga tctaagatga
     7981 ctaggttttt gatttggtat ttttagtttt agattgggtt aatttaaaaa ttttggttta
     8041 ttctaaaaat atagagaaga taatttaaaa actacaaata atcattgagt ggcaaaacga
     8101 aattagcctc tgcttgcccg aagagggata atatggaatt tgacgaacac tttaagcata
     8161 tattgcaatt ataccaagac tcacccgata ttcggatctt gaaattagaa tatccaattc
     8221 caaataaata tgaaactgca caagaagagt ttttagaaaa gtttttccaa aacaatcctt
     8281 ttgaaatttt tccaaatgat ttggaaacct ttacaataca aaatgcaaag attaaaagac
     8341 tcaaatttga tagaagacct tcaacattta ttggaatttt acgttttatc gagctttata
     8401 ggcaacagtt caactcaatc aagaaaaatc tgagcctatt agcaagacgt tataaaacat
     8461 ttgagaaaaa tgatttactt gatccagaaa attctgtttt aaaaaatgaa atatttactc
     8521 agatacttgc agcaggaaga gtcttaaaaa aatggactcc acaagtagaa cttctttata
     8581 aaaatacgtt agtcaacata aatacaaacc tagctgaatc agaactagtc aatggaaaag
     8641 gaaaaggatt tgtcaacctg agaaaaatcc acaatgaagg atatctgaac caaacctttc
     8701 atccctatta ccaaaatctt ttaaattttt gtattatgtg tattgccttt gcaaccaaat
     8761 tagaagagaa taccaagaag tggaaaatag ctgattaatc actttttcat ttatcaacac
     8821 aattctaaat ttacttttct aaaccgaatc ccaaaattac tttttgtccc attgctataa
     8881 tcattttatt tttcttagaa tgatttattt tgattttaaa agaatatttt tataatactt
     8941 tttatctaac taaagtagat acatgatatt caatttgagt aaaattcaaa ataaaaattc
     9001 tagatactaa gaggtattcc acaataggaa atgagtcgaa attcggaaaa aaaactaatt
     9061 aggaatctcg ggatttattt tggatatttg atatttagaa aagtcattct attgcaaaca
     9121 aaaacagctc gcggaaataa cgcttacatc ttaattttct tttctgaaat attctcattt
     9181 gcctccttat tgattcattc tattaaaaac tttatgattg catttcataa ttttagaaac
     9241 cataaaaaag aatctggagg tttaggttct agataaacaa cttttaaaac aagaattctt
     9301 tgatgaaaaa aaaaagaaaa caggcatccc tttttaccaa ataaaactaa tttaaagaga
     9361 agcccgattt cccttgaatt gatataaacc tcattgacta aacaaataat aaacaatttg
     9421 tagtatatag cccgaatttc atgacataat gattgtattt gtttgtaaat tcgggacaga
     9481 aaatactaat tttataggaa attaaccaat gtcgttaaat gattttatct tcacctctga
     9541 atctgtttcg gaaggtcacc ctgataaagt atgtgatcaa atttcggatg ctattctaga
     9601 tgcttttcta gctcaagatc caaaatccag agttgcctgc gaaaccctag taacaactaa
     9661 tcttgttgtg gtcgcgggag aaataacaag cctcggaaaa atagacgcac aagaaattgc
     9721 aagaggcgtt ataaacaaaa taggctataa tgatatcagt atgggttttg atgcagagtt
     9781 tgcagtagtg caggcgcata ttcataaaca aagtcctgat atctcgcaag gcgtaacaga
     9841 aggacaaggt ctatttaaag aacaaggggc aggagaccaa ggattaatgt ttgggttcgc
     9901 aatcgatgaa accaaagaat taatgccaat gcctatccaa tattcccatg ctcttatgaa
     9961 aaaactcgct gacctacgac atggtaaaaa attaaaattc ttaagaccgg atgcaaaatc
    10021 acaagttaca gtgatttacc aaaatggaaa acccgttgct attgatacag tcgtaatctc
    10081 aacacagcat acaccagacg ttaagcacaa acaaattaaa gaagctgtaa ttgaggagtg
    10141 tatcaaaaaa gtaattcctg caaatttact caaaaaaacc aaatacttta tcaaccctac
    10201 tggaaacttt gtaatcggtg gaccacatgg agatactgga ttaacaggaa gaaaaattat
    10261 tgtagataca tacggtggct atggaagaca tgggggtggc gctttctccg gaaaagatcc
    10321 ttccaaagta gatagatcag cagcttatat gggaagatat atagctaaaa acgtggtagc
    10381 agcaggactt gcttctaaat gcgaagttca attagcttat gcgataggcg ttgcagaacc
    10441 tgtttccgtt ttagtggata catttggaac ttcaaaaatt cctgatgcag aaattgtaaa
    10501 aagagtaaaa gctaatttta aattaactcc tagaggtatc attgaaactc ttggtttatt
    10561 agagaaaggt agaaagtatt ctgacactgc aagttacgga catttcggaa gaactggcga
    10621 aacttttact tgggaaagaa cagacaaagc agaggcacta aaaggctaat gggtgcggtt
    10681 acaacaaata caagttcaga attaaaagca actagagatg catatggtga tgcgctcgca
    10741 gagctgggat cagaaaatcc cagcattgtt gtgctagatg cagatttatc aggatctact
    10801 aagaccaatg ttttcaaaaa gaagtttcct gatcgttttt tcaacctagg tgttgcagaa
    10861 cagaatctag taggtcacgc tgctggaatg gcactgacag gacttattcc atttgcatcc
    10921 tcctttgcga tgtttctcgc aggtcgcgcc tgggaaatcg ttcgaaatag tgtcgtttat
    10981 ccaaatttga acgttaagct agtcgcatcc catggtggaa tcacactggg agaagatgga
    11041 gcatcccacc aaatcgtaga agactttgca attatgagag ttattccggg gctaacagtc
    11101 atttgccctt ctgattataa tgagacaaaa ctagtcataa aaaaaatcgc agaatacaaa
    11161 ggaccggtct atgtaagagt tggccgccct ccaattccat tgattcaccg cgaaaattac
    11221 caatttgaaa tcggaaaagg tgaactccta tctgagggaa aagatgtatt aattgttgcc
    11281 tgcggtcttt tagtgggcga atctttaaaa gccattgaga ctttaaaaga gaaaggaatt
    11341 tctgtctctt taatcaatat ggcgaccatc aaaccaatag ataaagagtt gattttaaag
    11401 catgcaaaaa tatgcaaagc ggtagttact tgcgaagaac atagtgtaat cggtggatta
    11461 ggctctgctg ttagcgaact tcttggggaa gaatttcctg tccctgtatt gaagttaggt
    11521 atgaacgatg tctttggaaa atctggaact tggagtgagc tattagatta cttcgggatg
    11581 agagcaaaaa acattgtatc tcttgcagaa aaagtaatcc agatgaaagg aaaaaactaa
    11641 agcttttttt ataacataca agtttattta cttccctgta ctaagtttta aacttgtatt
    11701 tccgataaga atagagatac attttaaaaa tctatttata acaatgagaa atcatttact
    11761 gagtagattt taagtaattg aaaatctgtt tttattcagt tataaaataa atatttcttt
    11821 ctaaattttt ttggtttctt accattgatt tagatatgga tatatgaatg tatcaatttg
    11881 ggagaaaaag aaacaaatga tagatcctga gattcaaaca gaaaagattg taacccaaaa
    11941 caaaactttt cttgttgatt taaaaaagaa tcaagcaggg tattatctta aagtttccga
    12001 atggtcaaat agtaaaaaat cttccatctt tgttcctgct gaaggaattg ataaattgat
    12061 tgaaattctt tctagattta agaccttgat agcaaatgac aaagaatgag ataactatgg
    12121 actttataaa gcactaaatt attccataca aatgaggaag gagagtttcg aatgaagttt
    12181 aaaaatatta ttatggtggt gttaatggca atgctcggat taccgtcgct ttctgcacaa
    12241 gaggcaaaag cagctaagtc cactgaaagt agcggaggcg gagcagcaat tgaacctttg
    12301 gaaagagtta ctttagaaga ctttgaagag tccgaagact ggagagcaaa atcaactact
    12361 ccgctgggac agacaaaagt tctaaagatg attcaaagag gaattatcaa agatgttttt
    12421 gatgaaaaat cagttccaga taaaaagaat gaaaaaggtg aatatgttcc tgtagcagaa
    12481 gaccaaaaag acagaatcgg aacaaatcat atcctcggtg taaaaacata ttttgcagaa
    12541 aaaggctttg atagagttga actctctcca cctcatgaat ttatcataaa agggaaagca
    12601 cgccagatat ctgtatgggt tttaggaaga aaattcaacc atacaatgta tgcaaaattt
    12661 agagattaca aaggcaatac tcataatatc agacttggta gactcgactt ctttggttgg
    12721 agaaaattaa ctgctccaat tcctggatat gttccacaaa gcacaagata ttctttgctt
    12781 gataaaaaca tccattttgt atcgttattt gttacaagcg atgttcatga agcagctgga
    12841 gatttttact tttatgtaga tgacttagaa gtaagatctg acaaatatga tatgatctat
    12901 cctggatttg aaattaagga taattggtag aatttattcg gaaagaggga atattatgaa
    12961 caataagatt aaatatttaa gtattacatc agcagtccta gcagcagtag ttgctttttc
    13021 cggaattgct tttgcagaaa acaaagggct caaaacaact aatggaaatg acttaggatc
    13081 aaatgagctg aaatcaatta cactagaaac atgggataaa acagaatggg aagtattcac
    13141 agataaggat tctgaaattc cagatagtta tggaaaaaaa ccctacaaaa aagatttacc
    13201 tccatcttct caagcaatga gacaagtaaa acttttaaaa ggttatccta gagacgttaa
    13261 atacattgat ttaagtaaag ataaagaaaa ctcacaagtc ttagcaatta aatttcattt
    13321 tacctttcca ggcaataatg aggttacaat tcgtccacct agaactgcag atttcttaat
    13381 caaccgtcca agaatgttta tcaacgaaaa tgctttttca agcgaagaag caaagaaaaa
    13441 cccagagaaa agagatacaa acaatgtaaa cgttcaacca atctatggag ttgaattacc
    13501 aggtgttact aaatctatgt ctgtatgggt tcttggtcgc ggtattgact ataacttaga
    13561 aggatggttt gaagactaca aaggagatac acacattatc aaatttggat ctgtaaactt
    13621 tgttggttgg agaccaatga cagctgtaat tcctgcaaat ataccacaat ttgtggaagc
    13681 ataccctgca accaaaagct tagttttcaa acaatttaaa ttaagatcta ccccaaaaac
    13741 aggtggagat gtaacttatt tattctttga tgaattaaag attttaacag atacatttga
    13801 tgttcacttt gatggagcaa atcttgactt tgatccagaa gattgttcaa acaaacaaag
    13861 agtagaagaa gtagaagcta aagctaaagg tattaagcca gttaaggatt gtggaaatcc
    13921 aggaagcaaa caggctgaaa atcctgctaa aaaataatta gaaattttac tacctctagt
    13981 aatgggaaaa tcctcgcaag tatttgcggg gatttttttt attctcacca aatcgactta
    14041 aatctaaatc aactcctctc aaccagcaaa cctttctaaa ataaaacgat ataattcaaa
    14101 tcttactcat ttttcatttg aattaaagtg caatcaatga ggatccagga tataaatgtt
    14161 tttatccgag tatcttaccg aatacattcc tgaattgaag gttcagtcct aaaacaccat
    14221 cttaaaagtg atgctttggt ttttaatcta agctgttgag gagtagactt tttcagttct
    14281 tcagaatcca taaaatgttt ttcattttta agttcgttgc tcccttagtt caacaaagga
    14341 aattggttct ttgaataaat caaaatgtat ctttcttatt atcctagctt ttcacatgtg
    14401 caaggatcaa atggattcgc atttcattca aatcacctct caatttctac caaatgtttc
    14461 tttaataagc attttctaac atagatacat tgcagaatac aaagaatcag cttataaaca
    14521 agcggttggc aattaattta tagtttactt atttccttag cttaaaatat tagaataaac
    14581 gtcttctttt atatataaat atcaaaaaaa aataaatctt taattgcttt tggagtggtt
    14641 tcatgtctca agaaacagtc attactaatg aaaaatcaag cagtgaatta gataaactga
    14701 caagtctttt taatgaagaa atatatgtca gggtagatgc ttcttctgtt cctgttacaa
    14761 agtttaaaat cctagatgat ttaatcgcgc attacaaatc tatcggtaag atagaagaag
    14821 cgaatcagaa agtagaggag cacttaaaag accacccaga aagtatttct gctcgttatt
    14881 taattggttt catctctctc attcaaaaca aaattgagga ttctaatcac ttaaaatctc
    14941 ttttagaaca atttaaaatc cacgggaaat ggactattat tgaatatata tctgatcata
    15001 tcttacaatt tggagagcaa agattagctc ttaaattcaa agcagaagct ctagaaaaat
    15061 taaacaaaaa caaagaatta aaactagttc tagagaaatt agcgaagcat gatcgaaaaa
    15121 atcccgaaat cgctaaaaaa tatgctctct caattttaga tgaagataga gataaagcga
    15181 ttattttcct aaaacaagca gcagaatctt ttgctagatc aaaagaatat ggccaattag
    15241 aagaaatttg gcctatccta atccaacata attttgaaga catacctttc tttgaaaaaa
    15301 ttgagcgaat cttactctct gctcgtgaaa aatctagaat tgtaatttta ttctatcctc
    15361 taatggaaac ttacaagcaa ttagaagatc atgataaaac aattttactt ctcaagaaaa
    15421 tcttagatca cgaacctctc tcgcaaaagg caagaaatga tttaattaga gcatataagt
    15481 caaaatatgc aaaccattcc ctattagaag agttcttaaa aatgtccgaa atcgggaata
    15541 gcaaaaaatc aatcaaggct tgtatcacaa actttgagag aaatattgtt tttgatacca
    15601 acaattatgt aatgcataga aattggggag ttggacagat taaatcaatt agtagttcta
    15661 gcgactcgat tgtagtagat ttccttggca aaaaagatca taaactttcg attcaaatgg
    15721 ctatcacaag tcttaagcca ttaaaacaag atcatatttg ggtaaaactc tacgagaaca
    15781 aacaacaaat tgtagattta tttaataatg atattccaaa cttc
//
LOCUS       DAOQOS010000122        15658 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_1901_length_15658_cov_4.623021, whole genome shotgun sequence.
ACCESSION   DAOQOS010000122 DAOQOS010000000
VERSION     DAOQOS010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 15658)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15658)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15658
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1901_length_15658_cov_4.623021"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(34..960)
                     /locus_tag="PLF66_20490"
     CDS             complement(34..960)
                     /locus_tag="PLF66_20490"
                     /inference="COORDINATES: protein motif:HMM:NF018125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1073 domain-containing protein"
                     /protein_id="HMZ66481.1"
                     /translation="MKKKLAKRYDSFTDEMTGRGTKKDKLSQITANPTSYHPVDLRKF
                     YQANGFIQNIVDGPADDATREWITIKTNRDDIKINRIIENRLLELKARAKIRELIRYM
                     RMYNQGGILYYGVKCLVPQTDTQLTEPIQTPIQSLDFINVIPPDRFNIYCDLYDPLAR
                     GYGKSRIHINGIEIHPTRYSWLVNSLIPEEHNGISVIQTILDAILAQDTALWSVNSLI
                     YELAVTVFKSKEVPNMKPAEVEEFLTTMKAVMSSQSAIALGSDEDLTKKTLSIPGVKE
                     LFEFIFDNLAGMAKMPKSRLMGQAQGTELLHI"
     gene            complement(957..2408)
                     /locus_tag="PLF66_20495"
     CDS             complement(957..2408)
                     /locus_tag="PLF66_20495"
                     /inference="COORDINATES: protein motif:HMM:NF015211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HMZ66482.1"
                     /translation="MPDKNVIHEILLEGQKKFAKSKIKPSDKKAYGKMHGREDLLAFA
                     RFIDPEFQTPPHIEEIAKKLTLAAEGKIKRLIINVPPGHGKSHLCTEIFPTWYLGNHP
                     THDVMVASYASSLAAGFTRWQRNTIESDSYREIFPEVYPGDKWTEDDWELLQGGSGMG
                     AGVDGGFTGRRANILIIDDVYKNFQEAMSDVKKETVWNWYRFVIRTRLAPNGVIIIVN
                     TRAAKDDLSGKLLERGKWEHVVFQALNPDGSVLFPTLYSEKEINELREDLGEDIFQTV
                     YQQNPQVNKKAKLLSEIRISDDVKIHSSTKRFAYLDPGGFGDGSNVDYNAVSVGCQIP
                     LNPPLQKGVLEEGIHVMFGDIWESFDETYDRVEAICKEFNVSVLYMESNAAQGTFATE
                     FRKRGIIVEKVHSSNNKHLRIVTHLKNNLRGVTFLHRCTPEYIKQITDYSLSSKHDDA
                     PDSAAGLIRAILPESAGNLKERYSFIKRLWRRR"
     gene            complement(2542..3078)
                     /locus_tag="PLF66_20500"
     CDS             complement(2542..3078)
                     /locus_tag="PLF66_20500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66483.1"
                     /translation="MYPQSIKTRAYHLFVTKGYTCERIAKELRKEYPKITGNTIRKWS
                     EERTDNNSQTWIEQKNSIEKNVVERINEKTISAIVDIRAKNKILIQEIFKRIIDKSAP
                     EAKSFEGLIYAFKAISSFEAQLEKDAGEKDKLSPAQVIQIILEIFQANPKIANVIRAE
                     WHSVSEQLHQQLLDEPKS"
     gene            complement(3078..3290)
                     /locus_tag="PLF66_20505"
     CDS             complement(3078..3290)
                     /locus_tag="PLF66_20505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66484.1"
                     /translation="MSENKGFLCVNDKIKPSSPDYTGKINIDGINYSISAWENTSRKT
                     GAKYLNIKVNPVGNSTVSDFSEDGEF"
     gene            complement(3277..3555)
                     /locus_tag="PLF66_20510"
     CDS             complement(3277..3555)
                     /locus_tag="PLF66_20510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66485.1"
                     /translation="MMNNIRLWVLRNAIDYSNGALVLGLFWFLAWGLWFRVFISYPEI
                     CHRWTPINTDRFFESFFFGVQLIILSLLFNWLYEFVNYLDNRKFNERK"
     gene            complement(3552..3950)
                     /locus_tag="PLF66_20515"
     CDS             complement(3552..3950)
                     /locus_tag="PLF66_20515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66486.1"
                     /translation="MILTNRGNACMRSILILFLLGCSSVQSNLSKLDLSAEKQMILDS
                     ADIPEPKKKFIVNTFDKAESFCNDAVSLVNSLHEVIEKQKTKIAELDEQAGIYRFWRN
                     ALILLCVLFVLYELRGFILPIVRKLMGIPI"
     gene            complement(3950..4123)
                     /locus_tag="PLF66_20520"
     CDS             complement(3950..4123)
                     /locus_tag="PLF66_20520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66487.1"
                     /translation="MIDWFMNLFIALLLMAVLAFVVVNTYSIYSTIQFQQKMILELEY
                     KCGWYIDDDKEKD"
     gene            complement(4162..4809)
                     /locus_tag="PLF66_20525"
     CDS             complement(4162..4809)
                     /locus_tag="PLF66_20525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66488.1"
                     /translation="METKNFQAIANIPFIGVKEYLTPDTQRDAVKLNKTGGATIAEGH
                     NHCKNTSCTMAWNALGKILGAQFPVLQSMATMGEYQYQAILANFMHPNEKIQDWQPHV
                     RMFNDLFRQNQIPLVAKFGFYRSNEQRIAASMAHGFPVVLGTMITDAGHIVLLYRRIQ
                     IEGMTFNVIIDPYGNPLKLYRGQGNDVADYYSIDIQHFNQWITSVCNCIWFEAIA"
     gene            complement(4818..5273)
                     /locus_tag="PLF66_20530"
     CDS             complement(4818..5273)
                     /locus_tag="PLF66_20530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66489.1"
                     /translation="MMKSPLPPFAKGGVDVGVSSTAKEDKLDKIAERVYNASFVITVL
                     LILGASIGTAVIRYISNDSKIELVPDNVFKFIEHILWIFALPVSVKIIGDRLPLVVQA
                     LQAWRGGGYGQTGFGQSGYGIQSSFGINQFGNTTTYPPTGDGANSNTYY"
     gene            complement(5270..5521)
                     /locus_tag="PLF66_20535"
     CDS             complement(5270..5521)
                     /locus_tag="PLF66_20535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66490.1"
                     /translation="MTKIKFCEVPIGEPFVYNDILYVKTKEDEGASQRLGPFTFHANE
                     IVEIAYPVERDPLKGSALRWLLVYSVIFGTLYLIGEFCL"
     gene            complement(5518..6126)
                     /locus_tag="PLF66_20540"
     CDS             complement(5518..6126)
                     /locus_tag="PLF66_20540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66491.1"
                     /translation="MEYLFVKVFRFILEYGVVQFLLGSFVFSIIRAVYKFFTNREIYN
                     DVIRSGRVKSILLRLGETKALLNCSKILITHIHNGAKWINGRHIYKLSIYKTLSLYSR
                     NNIETALSDIKLSEISEIIEKLNGRNFDIISVSELPNDFPFKRILKRDKISYIVLFKI
                     QKNSQALGYIWVIFAEGKEVTHDREVYSELQFLCDEIAEELK"
     gene            6853..7146
                     /locus_tag="PLF66_20545"
     CDS             6853..7146
                     /locus_tag="PLF66_20545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66492.1"
                     /translation="MDITTLQKIELDRETTVKKFREIGELKYDREQSKRYAEEAFKSR
                     YEDIKTKEDFICKGYFLIEKILGELNCMEEDNTDLRQQLFKFRKDFVLQNFSE"
     gene            7428..7712
                     /locus_tag="PLF66_20550"
     CDS             7428..7712
                     /locus_tag="PLF66_20550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66493.1"
                     /translation="MKTSEIAVNTINQLNEVCSNILNVDSVDEDYLDEVNFAIRMLKK
                     SIFNHIVKYEYSDFYLRNRSKKMNAKKFIDNPEKTLELFQNPKIRNSKKK"
     gene            8285..8506
                     /locus_tag="PLF66_20555"
     CDS             8285..8506
                     /locus_tag="PLF66_20555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66494.1"
                     /translation="MKQFKVIITDENGKITSTNFEGKSLEIFREVFSDKEFINRMNFL
                     KEKFIYKLGEEMSKLEFDEIVAVWKTMLS"
     gene            complement(8719..9168)
                     /locus_tag="PLF66_20560"
     CDS             complement(8719..9168)
                     /locus_tag="PLF66_20560"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HMZ66495.1"
                     /translation="MSASERLKRIVKHCGLKNSEFAESVGLSSGGLSDILSGRNKGIS
                     NPVVKAIQYRYNVNPTWLLTGEGEMFLSGTPQKKEEPYLYNAEDGVFIQGDKVITGRA
                     KAKAALEAAQARENKSQSVGDSVSLDGLDEEQRKIILSMIKQFRKGK"
     gene            9299..9490
                     /locus_tag="PLF66_20565"
     CDS             9299..9490
                     /locus_tag="PLF66_20565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66496.1"
                     /translation="MNNKKPNTIGDFRKRYENALYKPTLVERIKNFFRPKKTYIYIVQ
                     DLSNPECVHKLTFREGGAK"
     gene            complement(9671..10096)
                     /locus_tag="PLF66_20570"
     CDS             complement(9671..10096)
                     /locus_tag="PLF66_20570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66497.1"
                     /translation="MDKRDEINKYKKMAYDLAKKFTDTCEDHPNEKITQTLDRISNGI
                     CQALIMKPLGEFAFQPIALWEMDATQIKDAIFTILEEPDIEDTEEDIFYLAYFVEYEL
                     ISFQAVEASQIVQYAALMGLHPRWVWIHDEVLKRGDAHS"
     gene            10138..10632
                     /locus_tag="PLF66_20575"
     CDS             10138..10632
                     /locus_tag="PLF66_20575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66498.1"
                     /translation="MSSLLAKLYQQFADMPVQKFGFNNHSGEYQKYTNRSKSGRRPSQ
                     RLAVIIKVSDRTLSDIARIYAKNLPQHYRKKSVNVVVVKTNLSHVLAGRDNSPAYIKA
                     IEEAWRLPIAEIRAIYNEDRQRETPLTSNELHQFFLEYKARYSGVTLAEYLDKRNQIL
                     QGGI"
     gene            10632..10832
                     /locus_tag="PLF66_20580"
     CDS             10632..10832
                     /locus_tag="PLF66_20580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66499.1"
                     /translation="MIENGLEIKQAIRKLGYTLKEFAEAAEIDVNYLYNTINGYQEYR
                     EVLSALEKYGIKYVRKQRKRVA"
     gene            10829..11014
                     /locus_tag="PLF66_20585"
     CDS             10829..11014
                     /locus_tag="PLF66_20585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66500.1"
                     /translation="MKIKNKRDVSDQILDKFGTLESFAEKTGINYQLVVDGLVSTGHA
                     DKEFIETLQENGIRVEI"
     gene            11011..11190
                     /locus_tag="PLF66_20590"
     CDS             11011..11190
                     /locus_tag="PLF66_20590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66501.1"
                     /translation="MKDPNYLASYIARYQRRMNRIYRWQLFYEIAFHTISILFIWIVC
                     LLILEQAGINQGVLI"
     gene            11785..12039
                     /locus_tag="PLF66_20595"
     CDS             11785..12039
                     /locus_tag="PLF66_20595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66502.1"
                     /translation="MKLQLAIIEHFLMTAGHRWTADECALVVGGKKSSVYRALKNLSK
                     KNFIIHDRGATLYKLHPRFIEPILSYEKILKKEIQWKKRK"
     gene            12021..12914
                     /locus_tag="PLF66_20600"
     CDS             12021..12914
                     /locus_tag="PLF66_20600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66503.1"
                     /translation="MEEKEVEIVESGLQSDATVEAGLQPDVNQERLLRARMLIENINT
                     SMYLTLSHLKQFRDEELYKDLAFNSMEDFCNSGRLLFGYATAKKFLLVADKFGTDHVI
                     RQLSTDNDKMETLVRIANDPTIMQNYVQQEGIVKMPDGSEVTLKELTENIATSVKDQI
                     LAEEKESVKDLKKESKRLASENKNLAAQVELQKSMAETNHLEAAKWRETATKALGENI
                     DIEKFALLNTKEGAIKTIVNATEMLTAQISLVNEIPESMEPTIELIALANTFSASLKA
                     IQDRFLEAWNPYFFSTEISGE"
     gene            12920..14635
                     /locus_tag="PLF66_20605"
     CDS             12920..14635
                     /locus_tag="PLF66_20605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66504.1"
                     /translation="MKRNLDPTLVSQFYKEHKAAGRLQRGKIIEDYVALTGLSKPTLH
                     KIIKQMNIGENPFIVAQGAKTRGSSRKPKHIQEIEKKDAMVIAAYKKANFQASTQIAI
                     EVCYKQRLISCLYERSTMDRLLDKYGISARHFEKSHKSIHVTAPYPNRVWYVDASPME
                     QYYFEFKEKGKETNIIYLNEKSKDKHVSDLIQNKKLRKIWVYYAVDLYSKAYYLLPFA
                     GDPSDADAGMLGENHRDRLAFFQKLFLPKSPVKISGVDHIIPFEGIPEAIYSDKGFST
                     KSIQGIMSRFNCVVSSHMAGNPAAKGIVERRIGAFKQQYERILPRALIHSFEDLVDFL
                     HQWMVFDNTKKGYYQAYLQGTKIKPIKTIVLQDWYNVTTEKTNRDVDASGRVRINGKF
                     FTIEDSDILKGTKVSIFSNISGAMIAYDSKGNPHKIVEGDSLSTEFGNYKRKPDTDKD
                     RNRKEVEELGKKINKEIILESILPNPINVTHFPVKHTQGINTNSPIAPEFFTTVESAI
                     LFILEATKLAQEEIDPIKWHDYIDMMKQLQAKFGKIQSVFIYECIKDLNKNRIYEFKM
                     EATND"
     gene            14628..15581
                     /locus_tag="PLF66_20610"
     CDS             14628..15581
                     /locus_tag="PLF66_20610"
                     /inference="COORDINATES: protein motif:HMM:NF024793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HMZ66505.1"
                     /translation="MIKDNLAIAKTDFVKTRESERIMKAAHKAVDKNAWILTLGDVGM
                     GKSTIQRQLVSFWKEHSDRFAVMEFNGFKIKTSRINVMMKKMIRQVKPGEHIPGDIET
                     RYEVFRAAMANAVQKKIKPIIVIDNAQDLSNDTLLELKKIHEVSALGLEHLFTIVMFG
                     KYDLRFEENFKTRELRHRVQRFYLEKPSDAEIFEIARQTFGLTFEKGENKQLVEKIFL
                     RKSEGTPSGVKHIYNKLNFQNDFDGVITLNRLRNSFEVSYVDELKKAGLTVNHVLKAY
                     RDRFKKDIDKSTLSRALNHERDDKTAQNIRDLYNDIMGQTE"
BASE COUNT         5303 a         3235 c         2908 g         4212 t
ORIGIN      
        1 atatgagaag caatttgcct aaaatcgtgt ccactaaata tgtagtagtt cagttccctg
       61 tgcttgtccc atgagacgcg acttaggcat cttagccata ccggcaagat tatcaaaaat
      121 aaattcgaag agctctttca cacctggaat cgataaagtc tttttggtaa gatcctcatc
      181 gctaccaaga gcgatagcag attgactgga catgacagct ttcatcgtag tgagaaactc
      241 ttctacctcg gcaggcttca tatttggaac ttctttcgac ttaaagacag tcacagcaag
      301 ctcatagatc aaactattaa ccgaccacaa agctgtgtcc tgagcaagga ttgcatctag
      361 gattgtctga atcacagaga taccgttatg ctcctcaggt atgagagaat tcacaagcca
      421 gctataacga gtaggatgta tttcaatccc attgatatgg atccgagact ttccataccc
      481 acgagcaagc ggatcataca agtcacaata aatattaaaa cgatcaggag gaatgacatt
      541 aatgaaatcc aaactttgaa tcggagtttg aatcggctcg gtcaattgag tatccgtttg
      601 agggacaagg catttcactc cataatataa aatcccacct tggttataca ttcgcatgta
      661 gcgaatcagt tcccgaatct tcgcacgcgc tttgagttca agcaaacgat tctcgatgat
      721 acgattgatc ttgatatcat ctcgattcgt tttaattgta atccattccc gagtcgcgtc
      781 atccgcagga ccgtccacga tgttttgaat gaaaccattt gcttggtaaa actttcgaag
      841 gtcaacagga tgataggaag ttggattcgc tgtgatttgg ctcagcttat ctttcttcgt
      901 tccacgtcct gtcatttcat ctgtgaagga atcataacgt ttagctaatt tctttttcat
      961 ctacgtctcc aaagtcgttt aataaaacta tatcgctctt ttaggttgcc tgctgattca
     1021 ggtaaaatag cacgaatgag tccagcggca gaatcaggag catcatcatg cttagaggat
     1081 aacgaataat ctgtaatctg tttgatatac tcaggcgtgc atctatgcaa gaaggtaaca
     1141 cctcgcaaat tgtttttgag gtgtgttaca atccgaagat gcttattgtt cgagctatgg
     1201 actttctcta caatgattcc acgctttctg aactcagtgg caaatgtccc ctgagccgcg
     1261 ttagactcca tgtatagaac cgacacattg aactccttac aaatagcctc gactcgatca
     1321 tatgtttcat cgaatgattc ccaaatatca ccgaacataa cgtggattcc ttcctccaat
     1381 accccctttt gcaaaggggg attaaggggg atttgacaac caaccgatac tgcattataa
     1441 tcaacgttcg atccatctcc aaacccgccg gggtcgagat aggcaaaccg tttcgtggaa
     1501 gaatgaatct ttacgtcatc cgagatacga atctcagaca ataactttgc cttcttattg
     1561 acttgcgggt tttgttggta gaccgtttga aaaatatctt ctcctagatc ctctctcagt
     1621 tcattgatct ctttttctga gtagagtgta ggaaataaaa cagagccgtc aggattgaga
     1681 gcttgaaaga caacatgttc ccacttgcca cgctctaaga gcttgcccga taaatcatct
     1741 tttgccgcgc gagtatttac aataataatt acaccgttag gagcaagcct agttcgaatt
     1801 acaaatcgat accaattcca aactgtttcc tttttaacat cgctcatagc ctcttgaaag
     1861 tttttataca catcatctat aatgagtatg ttagcacgcc gtccagtaaa acccccatct
     1921 actccagctc ccatgccaga gccgccttgc aaaagttccc aatcatcctc agtccattta
     1981 tcacctggat aaacctctgg aaatatttcc cgataggaat cagattcaat tgtatttcgt
     2041 tgccagcgag taaatccagc cgctaacgaa gacgcataag aagcgaccat aacgtcatgg
     2101 gttggatgat tgcctaaata ccaagtagga aagatttctg tgcacaaatg agacttccca
     2161 tgtccgggag gaacgttaat aatcagacgc ttgatttttc cctcagcagc gagggttaat
     2221 ttttttgcga tttcttctat atgaggaggc gtttggaatt caggatcaat gaagcgagca
     2281 aaggcaagca gatcttcacg cccatgcatt ttaccataag ctttcttatc cgaaggctta
     2341 attttcgact tagcgaattt cttttgccct tcgagtaata tttcatgtat aacgttcttg
     2401 tctggcatat cgtgttgtca ttgtaaggaa cgcatatatg cgttccctat gatgacgcgt
     2461 tccctacgat gatgaataac gacaaatgaa aaacgatatt gtcatatccg tagggcgacg
     2521 gtcgcgaccg tcccctacat tttatgattt cggttcatcc aacaactgct gatgcaattg
     2581 ttcggataca gaatgccatt ctgcacggat gacatttgca atcttaggat tggcttgaaa
     2641 gatttcgaga atgatttgaa tgacttgcgc aggcgacagt ttgtcttttt cgccagcatc
     2701 tttttctagt tgtgcttcga aagaagagat tgctttgaat gcatagataa gaccttcaaa
     2761 agattttgcc tcaggagcgg atttatctat gatgcgttta aaaatttctt ggatgagaat
     2821 tttatttttg gctcgtatat caacgatagc ggaaatcgtt ttctcgttga tgcgctcgac
     2881 tacatttttt tcgatagagt ttttctgttc aatccaagtc tgagagttgt tgtcggttct
     2941 ttcctccgac cacttgcgaa ttgtgtttcc agtgatctta ggatattctt tgcgaagttc
     3001 tttcgcaata cgctcacagg tataaccctt cgtaacaaat aaatgatagg cacgagtttt
     3061 aatactttgt ggatacatta aaattcacca tcctcagaaa aatcagagac agtagaattg
     3121 ccaacaggat tgactttgat attgagatac ttcgcgcctg tctttcgaga agtattctcc
     3181 caagcagaga tagaataatt gataccatca atattgatct taccagtata gtctgggcta
     3241 cttggtttaa tcttgtcatt aacgcataag aatcctttat tttcgctcat tgaatttcct
     3301 attatctaaa taatttacaa actcataaag ccaattgaaa agaagcgaaa ggataatcaa
     3361 ttgaacacca aaaaagaaag attcaaagaa cctatcggtg ttaattggtg tccatcggtg
     3421 gcaaatctcg ggataactaa taaagactct aaaccaaaga ccccaagcca agaaccaaaa
     3481 gagaccaagc acaagagcgc cattggaata atcaatcgca tttcgtaaaa cccacaatcg
     3541 tatgttgttc atcatatagg aatccccatg agtttacgaa cgataggcaa aataaaacct
     3601 ctcagttcat agagaacaaa caaaacacaa agcagaatca aagcatttcg ccagaaacga
     3661 tagatcccag cttgttcatc tagctctgca atctttgttt tttgtttctc gatcacctca
     3721 tgcaatgaat ttacaagact cacagcatca ttgcaaaacg actccgcttt atcgaaagta
     3781 ttcacaataa actttttctt aggctctggg atatccgcag aatctaaaat catttgcttc
     3841 tcagccgaca aatccagctt actcaaattc gattgcacag aactacaacc gagcaaaaac
     3901 aaaatcagta tggaacgcat acatgcgttc cctctattcg taagaatcat tagtctttct
     3961 ccttatcatc atcaatatac caaccacatt tatattctaa ttccagaatc atcttctgtt
     4021 gaaactgaat ggtagaatag atactgtagg tattcaccac aacgaacgca agcaccgcca
     4081 taagaagcag tgcaataaac aaattcataa accaatcaat catacatcct ccttactgta
     4141 aggaacgcat atatgcattc cctacgcgat cgcctcaaac caaatacaat tgcaaacaga
     4201 cgtaatccac tgattgaaat gttgtatatc aatcgaataa taatctgcaa cgtcattacc
     4261 ctgacctcga tagagtttca aaggattacc ataagggtca atgatcacat tgaacgtcat
     4321 gccctcgatt tgaattcttc gatagagtaa aacaatatgc cctgcatccg taatcatggt
     4381 cccaaggaca accggaaacc cgtgagccat agaagcggca atgcgctgtt cattggaacg
     4441 atagaaacca aatttagcaa cgagaggaat ttgattttgt ctgaacaaat cattgaacat
     4501 tcttacatga ggttgccaat cttgaatctt ttcgtttgga tgcatgaagt tagcgagtat
     4561 cgcttgatac tgatactctc ccatagttgc catagactgt agaactggga actgtgcccc
     4621 aaggatttta ccaagagcat tccacgccat tgtgcaagat gtattcttgc agtggttatg
     4681 cccctcagcg atagtcgcac cgcccgtctt atttaattta acagcatcgc gttgtgtatc
     4741 aggagttaaa tactccttaa ctccaatgaa aggtatattt gcaattgctt gaaagttttt
     4801 tgtttccatg atatctccta ataatacgta ttcgaattag caccgtcacc cgttggcgga
     4861 tacgttgttg tattcccgaa ttgatttatt ccgaatgagc tttggattcc ataacctgat
     4921 tgaccaaacc ccgtttgccc gtaaccgccg ccacgccatg cctgcaaggc ttggacaact
     4981 aacggcagac gatccccaat gatcttgacg ctgacaggca aagcaaagat ccaaagaata
     5041 tgttcgatga acttaaagac attatcaggg acaagctcaa tctttgagtc attagagatg
     5101 taacgaatca cagccgtgcc aatcgaagcc cccaaaatca aaagaaccgt aataacaaaa
     5161 gatgcgttat acaccctctc cgcaatctta tctagcttgt cttccttagc ggtagaagat
     5221 acaccaacat caacgccccc tttcgcaaag gggggcaagg gggatttcat cataaacaaa
     5281 actctccaat caaatacaag gttccaaaaa taacagaata gactagtaac catcgaagag
     5341 cagaaccttt taaaggatct ctttcaacag gatacgcaat ctcaacaatt tcatttgcat
     5401 gaaacgtaaa tggtcctaga cgttgactcg cgccctcatc ctcttttgtc ttcacataca
     5461 gaatatcatt gtagacaaaa ggctcaccaa ttggaacttc acagaatttg attttagtca
     5521 tttcaactcc tcagcaatct catcacaaag aaattgcaac tctgaataca cttcgcgatc
     5581 atgagtaacc tctttgccct cggcaaagat cacccatata taaccaagag cttgcgaatt
     5641 cttttgaatc ttaaatagaa cgatataaga gattttatct cgcttcaaga tacgtttgaa
     5701 aggaaaatcg ttaggtaatt cagaaacaga aatgatgtca aaatttcgac cattcaattt
     5761 ttcaatgatc tcagaaatct ccgagagctt aatatcactg agagcggttt cgatgttatt
     5821 acgagagtaa agagataacg ttttataaat agaaagttta taaatatgac gtccattaat
     5881 ccacttcgca ccattatgaa tatgggtgat aagaatctta ctgcaattca gaagcgcttt
     5941 cgtttcccca aggcgaagca gaatagattt cactcgacca gatcgaatga cgtcattata
     6001 gatctctcga ttggtaaaga atttataaac cgcacgaatg atcgagaaca caaacgagcc
     6061 aagtaaaaac tgaaccacgc catattcaag aatgaatcga aagactttaa caaataaata
     6121 ttccataata acccttggat aaaaatccat gccgtttgac ggcatggtaa aacgagagac
     6181 accaatggtt attaatacgg aaagatattt aggattttgt cagatgctcg tagagacgca
     6241 cggcgtgcgt ctatgaagat ttaatttgat ttagaatcga cagtcgaaag aagttttgtc
     6301 tctcaccatt cgatttacaa ggaataagtt tggaaacgcg ataacgtgca atagaacgct
     6361 cagtaacatt cagagtgcga gcggcttgat gaatatccaa ataaaaatct gcatcgatgt
     6421 cagagaataa aagtggagtt tcctccatgc ggaaaatacc acgaacgcgt ttcttggtaa
     6481 ggacaggtcg cgacctgtcc ttactttccg atctgtcctg cgacaaatcc cgattaccca
     6541 ataaagaacc gttatacgta aacccagaca tgagaaacgt tttttacaga gccgaaaaaa
     6601 atgtcaagca aattcgtagg ggcgggtttc aaacccgtcc tacttttttg gtttcttctt
     6661 tcgcttctta ggttggatga tagttacaat tttgatattg gaatttttga tagaaatttt
     6721 cgacatagtg actcctgttc aagtgacacg ggttataatg gaagccactc aagcggtcaa
     6781 gagtttttta taaaaaacaa aatcggacac ataaaaaaag caactctcga aacttaagag
     6841 cagaggcaga atatggatat cacaacacta caaaagattg agctagatag agagacgaca
     6901 gtaaaaaagt ttcgtgaaat tggggaattg aaatacgata gagagcaatc aaaacgctac
     6961 gctgaggaag cttttaaaag tcgctatgag gatataaaaa caaaagagga tttcatttgc
     7021 aaaggatact ttctaatcga aaaaatacta ggtgagctaa attgtatgga agaggataac
     7081 accgatttaa gacaacagct ttttaaattt agaaaagatt ttgttctaca aaatttttcc
     7141 gaatgatatt tctgaaaaaa gtatcaggat ttttctgaga tataaattct ttatacgttt
     7201 cctctgggac gccaaaatac agagtcaaat gatttttact aaatacttcc aacgccccat
     7261 cctcatactg aacccaatca attaatttag aattaaaata atgcttagcc ataaacacct
     7321 ctttagattc attctgcatg ctctagcaaa tcggacaaac aaaaaaagca attctcgaaa
     7381 ctcaaagcac tggacataaa atcaaaactc ttttaaatga gataactatg aaaacatctg
     7441 agatagcagt taatacaata aatcaattaa atgaagtctg ttctaacata ttaaatgtag
     7501 attcagtcga tgaagattat ttggatgaag taaattttgc tatacgaatg ttgaaaaaat
     7561 caatcttcaa tcatattgta aagtatgaat attcagattt ttatttacga aatcgttcaa
     7621 agaaaatgaa cgctaaaaaa ttcattgata atccagaaaa gactttggaa ctttttcaaa
     7681 atccaaaaat tcgtaattca aagaagaaat aatttgttcc aactgggtga aacgttctcg
     7741 agtagagaga gtaacgcttg gtttccagtg atccccatca aaacaattct ttttaatttg
     7801 ccagtaatct ctcaccacac tatctagttt taattgatcg tctttagtta atttcttttt
     7861 agccataaac acctctctag aatcatcctg cacgctctag caaatcggac aaataaaaaa
     7921 agcaactctc gaaacttaag gcagagggaa aaagcaaccg ctatgaatac aaatcttttg
     7981 aaatggaaaa acattttatt tttcacaaag aaaagacaga tagagttatg ggaagatttt
     8041 aaaagtattt acttaaaaca gtttcctaaa tcgtccagtg agaaaggttc tttatgaaaa
     8101 cagcattatt atcatacaaa agattttgct tacctccaaa gctaagaaag cttgtagata
     8161 aatatcacag atacttatat gaggaattct atctcaagca ctccaatgag gatgcggtaa
     8221 gtttttcaga atttcaacgt agacatcaga aagctaatcc aagataattt ttagaggtaa
     8281 gagaatgaaa caattcaaag taatcataac agatgagaat ggtaaaataa ctagcactaa
     8341 tttcgaggga aaatctttag agatatttag agaagttttc agcgataagg aattcataaa
     8401 tagaatgaat tttcttaaag aaaaattcat atataaatta ggagaagaaa tgtccaagtt
     8461 agaatttgat gaaatagttg cagtttggaa aactatgcta agttaaatta actcgacgcc
     8521 ttgtaaattc ttgaaataat tcttgcgttg ttttcttatt caatatgtaa acaaatccac
     8581 aatgaggaca tttcaattca ctgtcctcaa ttattttaac attaagcatt gtgccattgc
     8641 aatttgtgca cggtaaatct actattattg ttttttcctt tgccatattt acttccttat
     8701 aattttattt tgaaacaatc acttcccctt cctaaactgt tttatcatcg aaaggataat
     8761 cttccgttgc tcctcatcca aaccgtcaag cgacaccgaa tcaccaaccg attgagattt
     8821 attttcgcga gcttgagcag cttctagggc agcctttgcc tttgctctac cggtgataac
     8881 tttatcacct tgtataaaca caccgtcctc ggcattatac aaatacggct cttctttctt
     8941 ctgtggagtg ccagacaaaa acatctcgcc ctcgccagtt aagagccacg ttggattaac
     9001 gttatatcta tattgaattg ctttaacaac aggatttgat atacctttat ttctaccaga
     9061 tagaatatcc gataacccgc ctgaagacaa acctacgctt tcagcaaact ccgagttttt
     9121 caacccgcaa tgtttaacta ttcgcttaag tcgttcagac gcagacataa aaatatttta
     9181 actttttttc gtattacgaa attttttcat tgacatttct cgcaatacga acgatactcc
     9241 ttatagagtg aataacttca ctcatcctaa aataaagaaa taaagaggca acaataaaat
     9301 gaataacaag aaaccaaata cgattggaga ttttagaaag cgatacgaga acgcgctcta
     9361 caagccaaca ctcgtagaac gaatcaagaa ttttttcaga cctaaaaaaa cttacatcta
     9421 cattgtccaa gatctttcaa accccgaatg cgttcataaa ttaacattca gagaaggcgg
     9481 tgcgaaatga agccaaaaac tatttatccc ttaccggacc taggtttctt tggttctcta
     9541 ttagccgtta tcaaaggtta tagagttcca aatcgacgaa ctacaaagga agttttagag
     9601 attattaata acaatcctaa gctcaaagct ctggcagaaa aagacaattc agaaaagaga
     9661 aagcaatgga ttaactatgc gcgtctcccc gcttcaaaac ttcgtcgtgt atccaaaccc
     9721 agcgcggatg taaacccatc aacgcagcat attgaacaat ctgagaggct tccacagctt
     9781 gaaaactaat caactcatat tcaacgaaat acgccaaata gaagatatcc tcttctgtgt
     9841 cttctatatc aggctcttca agaatcgtaa aaattgcatc tttaatttga gtagcatcca
     9901 tttcccaaag tgcaatgggt tgaaatgcaa attctccgag tggcttcatt atcagagcct
     9961 gacaaatacc attcgatatt ctatcgagag tttgcgtaat cttctcattt ggatgatctt
    10021 cacaagtgtc ggtaaacttt ttagcaagat cataagccat ttttttatat ttattaattt
    10081 cgtctctctt atccatacca accagcctag ggcgactaaa aacggaggca agcagaaatg
    10141 agcagtttac tcgctaagct ttatcaacaa tttgcagata tgcctgtcca gaagtttgga
    10201 ttcaataacc actctggcga gtatcaaaaa tacacaaacc gatccaagag cggtagacgt
    10261 ccgagtcaga gactagcagt catcatcaaa gtctcagacc gcaccctatc ggatatcgct
    10321 agaatctatg cgaagaattt accacaacat tatcgtaaaa aatcagttaa tgttgtggta
    10381 gttaagacaa acttatctca cgtccttgct ggcagagaca attcacccgc atacatcaaa
    10441 gcgattgagg aagcatggcg gctaccgatt gcggagattc gagctatcta caacgaggat
    10501 agacagagag agactccact cacatccaac gaactgcatc aatttttcct cgaatacaag
    10561 gctcgttatt cgggcgttac tctcgctgag tatttagaca aacgcaatca aattttacaa
    10621 ggaggcattt aatgatagaa aacggtttag agataaaaca agcaattcgc aaactaggct
    10681 acacactcaa ggagtttgcg gaggctgcgg agatagacgt taactattta tacaacacaa
    10741 tcaacggcta tcaagaatac cgagaggtct tatcggctct cgaaaaatac ggaattaaat
    10801 atgtccgtaa acaacgcaag agggtagcat gaaaattaaa aacaagcgcg atgtatctga
    10861 tcagattcta gataaattcg gaacgttaga aagcttcgca gagaaaacag gaatcaatta
    10921 tcagcttgta gtagatggat tagtctccac tggacacgca gataaagaat tcatcgagac
    10981 attacaagaa aacggaatca gggtagagat atgaaagatc caaactatct agcatcttac
    11041 atcgcacgtt accaacgccg aatgaatcgc atctatcgtt ggcaattatt ttacgagatt
    11101 gcctttcaca caatttcgat tcttttcatc tggattgttt gtttactcat tctcgaacaa
    11161 gcaggaatca accagggagt tttgatatga gacaactcga tttattttcg caagacaatc
    11221 cccctttatc cccctttgcg aaagggggcg tagtgcaaga gagtgacacc cctctttctt
    11281 gcccaccctt cgctaagtcg cgaccaacgc cttcgccagg ggacggggga gatttgaaga
    11341 gcctctcctc cgaacgcctc acccactatc gcacaaagct tgctatgtgg gacagaatcg
    11401 gctaccacaa agtagcgcag gaaatcgctg aggatacaat ccacggacac aacgaactgg
    11461 gagacgacat gcgtaaagtt gcaagtgcag ttaaacaaca tgtcccactc aactactttc
    11521 gtgactacag agcgaaagcg cggttacgca accgcgcctt atcaggagac accaaatgaa
    11581 aaaaacacgc ccctgcacac gatgcaaaat ccaaaacgta aagctctacc aacaaggact
    11641 ctgcaagcat tgtctacaaa aagactttga ccgcctacgt cctttgctat cagtcaaatc
    11701 gtcttcaacc aagacgatac aacaactacc gcaaaaggat acgctactcg ctagccccct
    11761 tttgcaaagg gggaacgggg gattatgaaa ctccaactag caatcatcga gcatttttta
    11821 atgacagccg gtcaccgttg gacagccgat gaatgtgcgt tagtagtcgg agggaaaaaa
    11881 agttctgttt accgagcgtt gaaaaattta agcaagaaga atttcattat ccatgatcga
    11941 ggggcaactc tatataagct gcatcctcga tttatagagc caatactgtc ttatgaaaaa
    12001 attcttaaaa aggagataca atggaagaaa aggaagtaga aatcgtagag tcaggtttgc
    12061 aatctgacgc aactgtagag gcaggcttgc aacctgatgt aaatcaagag agattactca
    12121 gggctagaat gctgattgag aatatcaata catcaatgta tttaactctc agtcacttga
    12181 aacagtttag agatgaggag ctatacaaag atttagcttt taatagcatg gaggattttt
    12241 gcaattcagg tagattacta tttggatatg caacggctaa aaagtttttg ttggttgcgg
    12301 ataagtttgg aacagatcac gtcatacgtc aactgtcaac agacaatgat aagatggaaa
    12361 cgttggttcg aattgcaaat gatccaacta tcatgcaaaa ctatgttcag caagaaggga
    12421 ttgtcaaaat gcctgacggt tctgaggtca cactcaaaga gctaacagag aacattgcaa
    12481 catctgtaaa agaccaaatc ttagcagagg aaaaagaatc tgtaaaagac ctgaaaaaag
    12541 aatccaaacg cttagcttcg gaaaataaaa acttagccgc acaagtagag cttcaaaaat
    12601 ctatggcaga gacaaaccat ctcgaagctg ctaagtggag ggaaaccgca accaaggcgt
    12661 taggggaaaa catagacatc gaaaaattcg ctcttctcaa tacaaaagaa ggcgcgatta
    12721 aaacgatcgt gaatgcaacc gaaatgctga cagcgcagat ttctcttgta aatgaaatcc
    12781 cagaaagcat ggagccaacg attgaactca tcgcacttgc aaatacattc agtgcaagtt
    12841 tgaaggcaat ccaagatcgc tttttggaag catggaaccc gtattttttt agcacagaaa
    12901 tttcaggaga ataaatgtta tgaaacggaa tctcgatcct acactcgtta gccaatttta
    12961 taaagagcac aaagcggcag gtagattaca gcgcggcaag atcattgaag actatgttgc
    13021 gttaaccggt ctatcaaagc caaccttaca taagattatc aaacaaatga atataggcga
    13081 gaatccgttt atcgtagccc aaggagctaa gactcgcgga agttcaagaa agccaaagca
    13141 tatccaagag attgagaaaa aagatgcaat ggtaatcgcc gcttacaaaa aagcaaactt
    13201 ccaagcgagc acacaaattg ctatcgaagt ttgttacaag caacgattaa tctcatgcct
    13261 ctacgaacgt tcaaccatgg atagattgtt ggataaatat ggaatatccg ctagacactt
    13321 cgaaaaatcg cataagtcga tacacgtcac agctccctat ccaaatcggg tttggtatgt
    13381 ggacgcgtcc cctatggagc aatactattt cgagttcaaa gagaaaggca aagagacaaa
    13441 catcatctat ctaaatgaaa aatcaaaaga caagcatgtc tccgatttaa ttcaaaacaa
    13501 gaaacttaga aaaatctggg tttactatgc agtagacctt tattcaaaag catactactt
    13561 attacccttt gcaggcgatc cgtcagacgc ggatgcaggt atgctaggag aaaatcatag
    13621 agaccgattg gcattctttc aaaaactatt tctgccaaaa tcgccagtta aaatttccgg
    13681 tgtagatcat ataattccct ttgaaggaat cccagaggca atctacagtg acaaaggatt
    13741 ttctacaaaa tccattcaag gaatcatgtc tcgcttcaac tgtgtagtat ctagtcacat
    13801 ggcaggcaac ccagcggcaa aaggaattgt agagaggagg attggagcgt tcaaacaaca
    13861 atacgaaaga attcttccac gcgcactcat tcactcattc gaagacctgg tagatttttt
    13921 acatcaatgg atggtctttg ataatacaaa aaaaggctac taccaagcct acctacaagg
    13981 gaccaaaata aaaccgatca agactatcgt cttacaagat tggtataacg tcacaacaga
    14041 aaaaacaaat cgagacgtag atgcatctgg acgtgttcga atcaacggca agttttttac
    14101 tatcgaagat agcgacattc tcaagggcac aaaggtaagt atcttctcga atatttccgg
    14161 tgcgatgata gcgtatgaca gcaaaggaaa tcctcacaag attgtagaag gcgattcact
    14221 ctcaacagag tttggaaatt acaagcgtaa gccagacaca gacaaagaca gaaaccgcaa
    14281 agaagtagaa gagcttggca aaaaaatcaa caaggaaatc atcctcgaat cgatcttgcc
    14341 taatccgatt aacgtaactc actttccggt gaagcataca cagggaatta atactaattc
    14401 tcctatcgca cctgaatttt ttacaacagt agaatcagca attttattta tattagaagc
    14461 aaccaaacta gcgcaagagg aaatcgatcc gatcaaatgg catgactaca tcgatatgat
    14521 gaaacaactg caagcgaagt ttggcaaaat ccaatccgta ttcatttatg aatgtatcaa
    14581 agacttaaac aaaaacagaa tttacgaatt caaaatggag gcaaccaatg attaaagaca
    14641 atttagcaat cgcaaaaacc gatttcgtta agacaagaga atccgagcga ataatgaaag
    14701 cagcccacaa ggcagtagat aagaacgcat ggatattaac cttaggcgat gtaggaatgg
    14761 gaaaatctac aattcaaaga caactagtga gtttctggaa agaacactca gatagattcg
    14821 ctgtcatgga gtttaacggc ttcaagatca aaacatctcg cataaatgtg atgatgaaaa
    14881 aaatgattcg tcaagtgaaa ccaggggagc atatcccagg tgacattgag acacgttatg
    14941 aagttttcag agctgcaatg gcaaatgcag tgcaaaagaa aatcaaaccg atcatcgtca
    15001 tagacaacgc acaggatcta agcaatgaca ctctcctcga attaaaaaag attcacgaag
    15061 taagcgcgct aggactcgag catttattca caattgtaat gtttggaaaa tacgatttgc
    15121 gctttgaaga gaatttcaaa accagagagt tacgtcatcg ggtccaaaga ttctatctag
    15181 aaaaaccttc tgatgcggaa atctttgaga ttgcaagaca gacctttgga ttaacgttcg
    15241 aaaagggaga gaacaaacaa ctagtagaaa aaatcttcct tcgcaaatca gaaggaacgc
    15301 catccggtgt aaagcatatc tacaacaaac tgaacttcca aaacgacttc gacggggtaa
    15361 tcactcttaa ccgcctacga aattcctttg aagtaagtta tgtagacgaa ctcaaaaaag
    15421 caggcttaac agtaaatcat gtcctaaaag cctatcgaga cagattcaag aaggacatcg
    15481 acaagagcac attatcgaga gctctaaacc acgagcgaga tgataagacc gctcaaaata
    15541 tccgtgatct atacaatgac atcatgggac aaaccgaata acgttttcat ataggaggaa
    15601 aaatgaaaat caccaacgaa caagaaaaac agcaagcgat tgcaagagca aagcaatt
//
LOCUS       DAOQOS010000123        15610 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_1906_length_15610_cov_6.477081, whole genome shotgun sequence.
ACCESSION   DAOQOS010000123 DAOQOS010000000
VERSION     DAOQOS010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 15610)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15610)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15610
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1906_length_15610_cov_6.477081"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..575
                     /locus_tag="PLF66_20615"
     CDS             <1..575
                     /locus_tag="PLF66_20615"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HMZ66506.1"
                     /translation="VSGFNTTNFTVSSDGGTTNHPGTVSISDKVVTFTPSINFNSNAT
                     YKVTLKAGSSLSTDYSFTFSTGNLSDTTPPSISSTNPLTGDTNMPINITISATLSETV
                     DPNTVNSSTMIVSGGVSGTVVLSDQTISFKPNTNLPVNTTLAVTIKSGIKDLAGNTMS
                     SQYSWTFTTGSTVATNCVFDTSTFDSCLYE"
     gene            630..1184
                     /gene="pyrE"
                     /locus_tag="PLF66_20620"
     CDS             630..1184
                     /gene="pyrE"
                     /locus_tag="PLF66_20620"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004421971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HMZ66507.1"
                     /translation="MNQKEELHKLIKTYAYRYSEQEFTLASGKKSHHYFNCKEIILVP
                     DRLSLFAEYVVNIHIPQVLGFQPESVGGLTLGADPISYSLALQYYRAGKIVYPLIVRK
                     EVKDHGTKKEIEGQIDKVKSCLVVDDVITTGGSTIKAVEALRRAGIKVESGICILDRE
                     EGGKEALAEIGVKMFPVFKKADFI"
     gene            complement(1177..1509)
                     /locus_tag="PLF66_20625"
     CDS             complement(1177..1509)
                     /locus_tag="PLF66_20625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66508.1"
                     /translation="MVDSKSVLTKIQNMDIREYFEKNIPHFNGNNKVIGVLKNGEELV
                     VAFEVTGLAPDQLQDFSTGMIFNLNHFKEFKNYSEKFVYSKTKNHHIKNNGSYVHGGQ
                     NIKRWQTK"
     gene            2076..2492
                     /locus_tag="PLF66_20630"
     CDS             2076..2492
                     /locus_tag="PLF66_20630"
                     /inference="COORDINATES: protein motif:HMM:NF014608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HMZ66509.1"
                     /translation="MAQLLDSDVIVSIKNEDYLSEVSIGKHRLVADEPESKGGQDKGP
                     NPYEMLLASLGSCTVITLRMYAQNKKWEIGTVQVKLNLEQVQRESEKITVIRRKIHFS
                     ENLESDKKDRLLAVAKACPVAKIISGTVEIDSALED"
     gene            2494..2919
                     /locus_tag="PLF66_20635"
     CDS             2494..2919
                     /locus_tag="PLF66_20635"
                     /inference="COORDINATES: protein motif:HMM:NF018595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HMZ66510.1"
                     /translation="MENEIEKKYTNQDITVIWKPKVCIHSAVCFKGLPGVFNPKKRPW
                     VTIEGATSEEIMAQIDKCPSGALSYVRNDSSGIEEETIKTQVEVMNNGPLLVYGTIEV
                     KDKEGNKTLKNKTTAFCRCGHSGNKPFCDGSHVKQGFKG"
     gene            complement(2937..3494)
                     /locus_tag="PLF66_20640"
     CDS             complement(2937..3494)
                     /locus_tag="PLF66_20640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66511.1"
                     /translation="MKTFFIILVSILFTYNCAITANTQKMTVTDFTSKKTIGENVFVE
                     ESTGGSITLPFWVSKISDENFTQAVKDSLINSKTFNKIVSNTNSDWKLKIVIIDHDHP
                     WFGFDMTVTTSINYTLFLKDKSVFSKTFVESGTATGTEMLIGVYRLKRANEYSAKNNI
                     KKFISELDSLDLKPETNTESPKLKK"
     gene            complement(3576..3968)
                     /locus_tag="PLF66_20645"
     CDS             complement(3576..3968)
                     /locus_tag="PLF66_20645"
                     /inference="COORDINATES: protein motif:HMM:NF024571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HMZ66512.1"
                     /translation="MDAPQVSKTTLSQDIMKHYKNPLYFNFDHPEDRIHIQKRNWKKE
                     NDLLVFDEIHKWKNWKSWIKGIYDNEKEDHAILITGSARLDVNRKGGDSLQGRYHYYS
                     LNPFTFSEIYEQKKSYQDIHIFFLFKTF"
     gene            complement(4095..5735)
                     /locus_tag="PLF66_20650"
     CDS             complement(4095..5735)
                     /locus_tag="PLF66_20650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000626146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HMZ66513.1"
                     /translation="MISVSGLAVRYGKKTLFEDVSIKFKEGCRYGLIGANGSGKSTFL
                     KILAGLEQPHQGVVSVDKGMRIGFLKQDHYEYENTTIMNTVLMGHKELWEVAQERDRL
                     YSLPEMSEEEGIKASELEEKYADLDGYESESYAGELLEGLGIPSNLHQETMSRITGGY
                     KLRVLLAQVLFQKPEILLLDEPTNNLDIKTIKWLEDFLRNHTGVLIVISHDRHFINSI
                     ATDIADLDYNVIRVYPGNYDDYMEASTLAREQLINENKRTKEKIADLQEFVSRFSANA
                     SKAKQATSRAKLIDKLKAGQIEIKPSSRVSPYIRFKMSKPLGKDVINAVGISKSYENP
                     VFKDVSISIAKGERVGIIGTNGVGKTTLLKCLLKIIEPDTGKVEHGASMSASYFPQDH
                     KDGIGEDAATLIEWLYRYAPPGTDTTVIRSMLGRMLFSGDMAQKGTSVLSGGEKSRLI
                     LSRMIMAEDNVLALDEPTNHLDLESIEALNYSLSLFEGTVIFVSHDREFVSSLATRII
                     EVTPDKVIDFKGTYEEYLEKEGAEFFKRAASGSLLAKK"
     gene            complement(5775..7118)
                     /locus_tag="PLF66_20655"
     CDS             complement(5775..7118)
                     /locus_tag="PLF66_20655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004784789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66514.1"
                     /translation="MNQKLNKLPSAFYILILSLFFLFNCANGASGSSGKTSIGKYAGV
                     PYAVLSTLFNNRMLLLLKLTYATDAPLNFSDYNNGTGQLYQDVQDTGAGGDPAFDLFQ
                     LPLAQNLNFFIDIGEIRISSKIQQSLTGLDYGVTSITASKKYWDFIATQRQVYCSAFY
                     SLGGNSCDLTSKTPLVLINQLFNGEGAKFPSNDPTATIPGNNGGIDVPTKYYASGIYV
                     RSFVTGWARQNGGLITNTIFDNNQIITGGQNIVPRNNYLPGTTDTDKSLKTPFMFPVF
                     HALKPGQEDMSIRGGADPYILEVRMNIKENLMVHSYTTTLGFVQTFIGISDWRYNHSG
                     QVDSGGNILSRSRIIYPETASSLQITGGTRSLQHYYNVFRDGETELVNELPLASTPVK
                     QGTSNIKYIHSGSYILVCAQDRSPVDGYPETVVRSTKFSVPETFRQTITVNLACP"
     gene            complement(7124..7717)
                     /locus_tag="PLF66_20660"
     CDS             complement(7124..7717)
                     /locus_tag="PLF66_20660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015681603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66515.1"
                     /translation="MLSSIPPLSAQQTEQAKPEIGFWIGAANPRPGSQTATVLDTTLG
                     FGLFTRFNWPYVFYTEIGAGMANYLSQTERSLFTVPIYTALCYKLPFELPVSIFLKAG
                     GGASYVIARPANTGRWDPMSILGTEVSFVAGRKVRIGLRVDYTVIYETFWVDKNPATQ
                     YPYLSPLDRDYRLSQPNQYQLKNGEFFNFALMVSFLL"
     gene            complement(7817..8638)
                     /gene="truA"
                     /locus_tag="PLF66_20665"
     CDS             complement(7817..8638)
                     /gene="truA"
                     /locus_tag="PLF66_20665"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HMZ66516.1"
                     /translation="MPSSVLILEYDGPQFNGFQKQESAKRVTVQGKLETILSQILREN
                     ITLVAAGRTDAGVHAKGMAVSFTSQNPIQNYGKLILSVNAIAGLEGGLAVLTANEVPD
                     GFHARFSCTEREYEYKILNTQTPHPLLKERALWFKNKIDFNLLQREMESLIGKHDFAS
                     FTKKSVLETYTSTERRITKIEIKEDEELKNLFKIVIRGTGFLHNMVRILVGTLLDIAR
                     GKIRSSVLEILESLDRTKAGKTAPPYGLYFVRAYYNDYPIVNHLYSQIYKNVEIV"
     gene            complement(8641..9525)
                     /locus_tag="PLF66_20670"
     CDS             complement(8641..9525)
                     /locus_tag="PLF66_20670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000127185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2225 domain-containing protein"
                     /protein_id="HMZ66517.1"
                     /translation="MPANTKVEPLKKASFRSKENSVCPVCDTVHQREQMFQGGGRLIA
                     GKLTKELRRLYEKNKKFGRINPNDYVIVVCPRCLYASFPKDWSTLAGPDLERMKFQSG
                     DRKVNLEKIIGPVDFSAERNLVTGAGSFLLAIDSYQNRSPKDAPTPKKAVCALKAAWY
                     FDDLHTEFPNLGYDKVRDTLYQKAASWYGLTLDIMQTGQEPVDSAAPILGPDTDNNWG
                     FDGVIYLNAYLTMKFRDMMSDIPEERVKLLAKSKRMLAKLYGSGKSSKSKPSAILDMA
                     KELYDELAKLIESLGGEK"
     gene            9813..11015
                     /locus_tag="PLF66_20675"
     CDS             9813..11015
                     /locus_tag="PLF66_20675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016544397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66518.1"
                     /translation="MKKIIISLTLIFGFSSLFAEPIMTKAYQKRVELLGYLRILEPIV
                     KNYRGNDKEGKPALLKAEVGKEGERVRRYNEIKKLFQEGLTDYFEGDYSNSYRRFLEA
                     QVDTEQLLEEISEFYIEGTAEILKAAVFAKDQKEYEDSAFDAKEGNKKVGDSLKDVDR
                     ELVDISVEYGRGSKNVGEFRENREAPYVSRQYDAKEFHFATNKYAIEQNTEAGYRALG
                     QAKKARIEALKIERNLERHQKLQPVHRKYRIEKYLDVIARCRDGREAAINLFRLKYPY
                     DNYYLQKDDKTTLGKVSLADAKGDEVALDKGKSMNFRLNPHVLPKNLNPVFDRRIPEK
                     YRRDASDLLGKVYEEEIEESVQLKFTDLNHVNSDLKRQLINETLVVEDGKDSPKGDSK
                     PAATEPKK"
     gene            11088..11864
                     /locus_tag="PLF66_20680"
     CDS             11088..11864
                     /locus_tag="PLF66_20680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016544517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HMZ66519.1"
                     /translation="MATLNPYKYLKQNLGDQSQDYHWLIVRTYMEVLVVIFTRAKRIL
                     LKGLFYSFLNKEKKAYAFLEGSWLWGDRMIKVTKTKLVLVNEIKIPERNHMIFVNHVN
                     ELDFPFDCYVIKKPYLANQQIKSSYFAYWWMKAMGSQVFDTSQARTIAKSVHELVEGL
                     KMFSYIVYPEGHNSYNEEIKPLKKGMVKVAFENKIPILVILKSGITKFQTSPKGNVIG
                     YKSAGIVDPTQFKTWEELKDHLFKLMSEEKKALDEYVAKA"
     gene            11861..13873
                     /locus_tag="PLF66_20685"
     CDS             11861..13873
                     /locus_tag="PLF66_20685"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF006367.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HMZ66520.1"
                     /translation="MKKIERLLIANRGEIAVRIIRTCKKLGIHSICIYSEADSDSVFV
                     KLADESHLVGPAQASESYLHIPKIIDICKKSKADAVHPGYGFLSESPAFAKALEEAGI
                     LFIGPSVESVRLMGDKIQSRIAMIQAGIPVVPGYDGKDQDGKLLLAHAKEIGFPVMVK
                     ASAGGGGKGMRRVDREEDFMESLLSAKREAKNFFGDDTIFIEKFISNPRHIEFQVFGD
                     KHGNAIHVFERDCSVQRRHQKIIEESPAFNLDNELRNKMGEVAVCVAKSISYIGAGTV
                     EFIVSDKNEFYFMEMNTRLQVEHPVTEMVTDLDLVELQIKIAEGDKLSVLPKLLEKHS
                     IEVRIYAEDPENNFLPSTGKILFLKNPSGDGVRLDSGIELGSEVTIYYDPMIAKLIVT
                     APNRSICIENLISALDQFIIFGITTNISYLKKILSHSEFKAGNLSTGFIDKYLKNDIL
                     IDNSLVDTIGYAHLLSNSKVQSQFVNDNFRNFQFWAKNAISNKLQTILFPDILNSISK
                     WKYRGELFEVKVLSFSETDSNFKFRISILNLDTKHYTERDLETAKEKFGNSIQTNLGE
                     IIFYQYGLTAFIHLNGTAYQLRREQRSVLASQNQSNTFTSPMPGKVIQIFVKEKDFVK
                     AGDTLAIVEAMKMENAIKSHKDCEVVEVSCNIGDLVRQDDILIRVK"
     gene            13936..14058
                     /locus_tag="PLF66_20690"
     CDS             13936..14058
                     /locus_tag="PLF66_20690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66521.1"
                     /translation="MESREIADFALKYSQKYPILIISGPRQSGKTTLSKLLFPK"
     gene            14195..14344
                     /locus_tag="PLF66_20695"
     CDS             14195..14344
                     /locus_tag="PLF66_20695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66522.1"
                     /translation="MKFFKNLHYYEKQDLQIQKKYLVYGGSINRIQSDVEVVSWEKAG
                     NVGLY"
     gene            complement(14499..14867)
                     /locus_tag="PLF66_20700"
     CDS             complement(14499..14867)
                     /locus_tag="PLF66_20700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66523.1"
                     /translation="MNDSHEKKFYLMSPVSLTTAKKTREATQMLREKPHEKDMPEKAL
                     RAIYNITEESLHYYFVRPLEILNLGPLSRGVVNMGVNAGLGVLNTFGKQLISHLSEKQ
                     LVQLADIIDQVLVELDSDKK"
     gene            complement(14936..>15610)
                     /locus_tag="PLF66_20705"
     CDS             complement(14936..>15610)
                     /locus_tag="PLF66_20705"
                     /inference="COORDINATES: protein motif:HMM:NF019245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1577 domain-containing protein"
                     /protein_id="HMZ66524.1"
                     /translation="DYIKIDIFKPDLEERFHVVKESGKTLYVANTQDRKCYTAFTDDD
                     FIDLEEEYHAEINRLISMYKTKNIVSEIIMPVIYVNTQNVAIPFGYVHIQSRTKPIDH
                     DQVMELKILTFEMIDRIRESNFILNTNKFRILDLSKSGLKIRVPDRELKQHLPHVTGF
                     NCDIFFKMQSAINIQCEIRFIGRDQNDDMILGLLITGFREGDKEKFYSNFSTLQNTPK
                     KPYHLP"
BASE COUNT         4999 a         2592 c         2759 g         5260 t
ORIGIN      
        1 ctgtttctgg atttaacact actaatttta ctgttagttc tgatggaggc acgaccaatc
       61 atcctggaac tgtttctatt tcagataagg ttgtgacctt tactccatct ataaatttta
      121 attcgaatgc aacctataaa gttaccctaa aggcaggttc ttctttatct actgattata
      181 gttttacatt tagcactggt aatctttctg atacaactcc tccctctatt tctagcacga
      241 accctcttac tggtgataca aatatgccta ttaatataac gatatccgca acattaagtg
      301 aaacagttga tccaaataca gttaattctt caacaatgat agtttcgggt ggtgtttctg
      361 gaactgttgt tctttccgat caaactattt cttttaaacc gaacacaaat ttacctgtaa
      421 atacaactct tgcagttact attaaatccg ggatcaaaga tctcgctgga aacacaatgt
      481 catcccaata ttcttggaca tttacaactg gatccactgt agccaccaat tgtgtttttg
      541 atacgagcac ttttgattct tgtttatacg aataggttca aaatattttt tttaaatgac
      601 agaggaggct attttgaaaa gcttcctcta tgaatcaaaa agaagaactg cataaattaa
      661 tcaaaactta cgcctatcga tattcagagc aggaatttac tctggcttct gggaaaaaat
      721 cacatcatta ttttaattgc aaagaaatta ttttagtccc agacaggctt tccctgtttg
      781 ctgagtatgt ggttaatatc catataccgc aagtattggg atttcaacct gaatctgttg
      841 gaggtctcac actaggagca gatccaatta gctattctct tgctttgcaa tactatcgag
      901 cgggtaagat tgtttatcct cttatcgttc gaaaagaagt aaaagaccat ggcacaaaaa
      961 aggaaatcga aggtcaaatt gataaagtca agtcctgtct agttgttgat gatgttataa
     1021 ctacaggtgg ctcaacaata aaggctgtcg aagcacttcg tcgtgctgga attaaagttg
     1081 aatctggaat ttgtatttta gatagagaag agggtggtaa agaagcgtta gctgagatag
     1141 gcgtgaaaat gtttccagta tttaaaaagg cagactttat ttagtctgcc atcttttaat
     1201 attttgacca ccatgcacat aggaaccgtt attcttaatg tgatggtttt ttgtcttact
     1261 atagacaaat ttttcagaat agtttttaaa ttctttgaaa tgatttaaat taaaaatcat
     1321 cccagtagaa aaatcttgca actgatcagg agcaagacct gtcacttcaa atgctacaac
     1381 caactcttcc ccatttttta aaacacctat tactttatta tttccattaa agtgtggtat
     1441 gtttttctca aaatattctc ttatgtccat gttttgaatt ttagtaagga cgcttttaga
     1501 gtcaaccatt ttcctttgca atttttatgc tttgaaagga atcgattttt cgagaatcaa
     1561 tattttttat ataggacatt ttctttaggg taaaaagttg aaatgattct tttctggttc
     1621 ctcttatttt atttgttaat tatcaaattc tttaaagtaa attgatttaa atttttggag
     1681 gacggttcta ataattgaaa ctgaaaaatc tattttaaaa aagactcatc attgtttaac
     1741 aaaaaaagga ttgagttttg gtgaaccaat cattcaagcg aggttttgtt tctattttaa
     1801 ttgaaaaatg acaatcgtct taagcagagc gaaagaggag agcttctctc tctcattttt
     1861 tcgctcttaa agactgtata tgaatgattt ggttaattca ttcgtcttct ctaatattat
     1921 aaaatcatcg cttgtttcta attttttaaa ttttgcgtat gctgagtaaa taagcctttt
     1981 taatgaataa aattttttta gaaaagttaa aaaccgtttc cattctagtg agtccttgga
     2041 aaaactagaa tctcttataa aaaaggggaa tttttatggc gcaattattg gatagtgatg
     2101 ttatcgtttc tataaaaaac gaagattatc tttctgaagt atcaatcggc aaacatcgat
     2161 tagtcgctga tgagcctgaa tccaaagggg gacaggacaa aggtcctaat ccttatgaaa
     2221 tgttacttgc ttctcttgga agttgcactg ttataacact tcgtatgtat gctcagaata
     2281 agaaatggga aattggaact gttcaggtaa aattaaatct agaacaggtt caaagagaat
     2341 ctgaaaaaat tactgtgatt cgtcgaaaaa ttcatttctc tgagaattta gaatcagata
     2401 aaaaagatag actattagct gttgcaaagg cttgtccggt tgcaaaaatt atttctggaa
     2461 ctgtagaaat cgacagtgct ttggaggatt aaaatggaga atgagataga aaaaaaatac
     2521 acaaatcaag atataaccgt tatctggaaa ccgaaagtat gtattcactc agcagtttgc
     2581 tttaagggct tgcccggcgt atttaatcca aaaaagcgtc cttgggtgac aatcgaagga
     2641 gcaacttctg aggagatcat ggctcagatt gataaatgtc cgtcaggcgc tttatcctat
     2701 gttcgaaacg atagtagtgg tatagaagaa gaaacaatta agacccaagt agaagttatg
     2761 aataatggac ctctgcttgt ttatggaaca attgaagtaa aagacaaaga aggaaacaaa
     2821 accctaaaaa ataaaactac cgcattttgt agatgcggac attctgggaa taaacctttt
     2881 tgtgatggtt ctcatgtaaa acaaggattt aaaggctaat ttatattagc ctcgttctat
     2941 ttttttagtt ttggactttc tgtatttgtt tctggtttta aatctaaaga gtctagttca
     3001 gaaataaatt ttttgatatt gttctttgct gaatattcat tcgcacgttt aagtctgtaa
     3061 acaccaatta acatttccgt tcctgttgca gttcctgatt ctacgaaggt tttactgaat
     3121 accgatttat cctttagaaa cagagtatag ttgatagagg ttgtgacggt catatcaaac
     3181 ccgaaccaag ggtggtcatg atcaataatc actattttaa gtttccagtc tgaatttgta
     3241 tttgatacta ttttattaaa tgtctttgaa ttgattagag agtctttcac tgcttgtgta
     3301 aaattctcat cgctgatttt ggagacccag aaaggtaaag ttatgcttcc gcctgtcgac
     3361 tcctctacaa aaacattctc acctattgtt ttttttgatg taaaatcagt aacagtcatt
     3421 ttctgtgtat ttgctgtaat tgcacaatta taagtaaata gaattgagac cagtatgata
     3481 aaaaaggttt tcattggaat atcctcaagt attttctttt taattgaatc aaatgaatca
     3541 tttgaataca attcattttt tatactaaat tgattttaga aagtcttgaa aaggaagaat
     3601 atatgtatgt cttgatagga tttcttttgt tcgtaaatct cactgaatgt aaatggattt
     3661 aaactatagt agtgatatct tccttgtagt gaatctccac cttttctatt tacatctaat
     3721 cttgcagagc cggtaattaa aattgcatga tcttcttttt cattgtcata aattccttta
     3781 atccaagatt tccaattttt ccatttgtga atttcatcaa aaacgagaag atcattttct
     3841 tttttccaat ttcttttctg aatatgaatt ctatcttcag gatgatcaaa gttaaaatag
     3901 agaggatttt tataatgctt cattatatcc tgagagagag tggttttgct tacttggggg
     3961 gcatccaatg aaaaccacct tctttgaata caaatcttct agtataaatt tctaaattct
     4021 tctgcgcata cagtaaaaaa agtatacacg gcaatcatga agttgacttg taatttgccg
     4081 gacggggaaa gtctttattt tttagctaat aaagatccac ttgcggctct tttgaagaat
     4141 tcagctccct ctttttcaag gtattcttca taagttcctt tgaaatcaat aaccttatcg
     4201 ggggtaactt caattatccg cgttgcaaga gaagatacga attctcggtc atgcgaaaca
     4261 aatatcactg ttccctcaaa taaagaaagt gaatagttga gtgcttcgat gctttctaag
     4321 tctaagtggt tcgtaggctc atcaagtgct agaacattat cttctgccat aatcattcta
     4381 gataggatca gtctggactt ctcacctcca gataaaacgc ttgttccttt ttgtgccata
     4441 tcgccactaa agagcatacg tcctagcata cttcttatca ctgtagtatc tgttccaggt
     4501 ggtgcataac ggtatagcca ttcaatgagt gttgctgcat cttctccgat tccatctttg
     4561 tgatcttgag ggaaataaga agcgctcata cttgcaccgt gttctacttt tcctgtatcc
     4621 ggctcaatta tttttaaaag acatttaagt agagttgttt tacctacacc atttgttccg
     4681 atgataccga ctctctctcc tttcgcaata gagatactaa cgtctttaaa aactggattc
     4741 tcataagatt ttgaaattcc aacggcgttg attacatctt ttccgagagg cttagacatc
     4801 ttgaatcgaa tataaggtga tactctagat gagggtttga tttcgatttg tccagctttc
     4861 aatttatcga ttaacttggc tctagaagta gcttgctttg ccttacttgc gtttgcacta
     4921 aatcggctca cgaactcttg taagtctgca atcttctctt ttgttcgttt gttttcattg
     4981 attaattgct ctctagctaa agtagaagct tccatataat catcataatt tcctggataa
     5041 actctgatta cattgtaatc taagtctgca atgtcggttg cgatcgaatt gataaaatgc
     5101 ctatcgtggg aaattactat taaaacacct gtgtgatttc ttaagaaatc ttcaagccat
     5161 ttgattgtct tgatatcgag gttgtttgtt ggttcatcta aaagtaaaat ttctggtttc
     5221 tgaaatagga cctgcgcgag taatactcgt aacttatatc cacccgttat gcgagacatt
     5281 gtttcttgat gaagattaga aggaattcca agaccttcca aaagctctcc cgcataactt
     5341 tccgattcgt atccatctag gtctgcgtat ttctcttcta attcacttgc tttgattcct
     5401 tcttcctcag acatctcagg gagagaataa aggcgatctc tttcttgtgc aacttcccaa
     5461 agttccttat ggcccataag aactgtgttc atgattgttg tgttctcgta ttcgtaatga
     5521 tcttgcttta aaaatccaat tctcattcct ttatcaaccg agacaacacc ttggtgtggt
     5581 tgttctaaac cagcgagaat ttttagaaat gtagatttac cactaccgtt ggctccgatg
     5641 agaccatagc gacaaccttc tttaaacttt atacttacat cttcaaataa agtctttttt
     5701 ccatatcgaa cggctaagcc agaaacacta atcatattgt cagaatttct gaacttatcg
     5761 cctagccaag caatttaagg acaggctaag ttaactgtta tggtttgtcg aaatgtttca
     5821 ggaactgaaa acttggtaga tcgaactact gtctccggat atccgtctac gggggatcta
     5881 tcttgtgcac agactaaaat atagcttccc gaatgaatat atttaatatt ggacgttcct
     5941 tgtttgactg gtgttgatgc aagtggtagc tcgttgacta actccgtttc cccatctcgg
     6001 aaaacattat aataatgttg gagagatctg gttcctccag tgatttgtaa tgaggatgct
     6061 gtctcaggat agataattct agatctagat aaaatatttc ctcctgaatc aacttgccca
     6121 gagtgattgt atctccaatc gctaattcca ataaatgttt gcacgaatcc aagagttgtt
     6181 gtataagaat gaaccattag attttctttt atattcattc gaacttctaa aatataggga
     6241 tctgctccac ctcgaattga catatcttct tggcctggtt ttaaagcatg gaaaacagga
     6301 aacataaaag gagttttgag tgatttatct gtatctgttg ttccaggaag ataattattt
     6361 ctaggaacaa tattttgtcc ccctgttatg atttgattgt tatcaaagat tgtattggta
     6421 atgagtcctc cattctgtct tgcccagcca gtcacaaaac ttctaacata aattcccgat
     6481 gcataatact ttgtaggaac atctattcct ccattgtttc caggaattgt agctgttgga
     6541 tcgttagacg gaaactttgc tccttctcca ttgaatagtt ggttgattaa tacgagtgga
     6601 gttttgctag ttaaatcgca gctatttcct cctagagaat agaatgcaga acaataaact
     6661 tgtctttgcg tagcaataaa gtcccaatat tttttggatg cagtaatgct tgttactcca
     6721 taatcaagac ctgtaagact ttgctggatt ttagaagaaa ttcgaatttc tccaatatca
     6781 ataaagaaat ttaaattttg ggcgagtggg agttgaaata agtcaaaagc aggatctccg
     6841 cctgctcctg tatcttgcac gtcctgataa agttgacctg ttccgttgtt gtaatcagaa
     6901 aaatttaacg gtgcgtcagt cgcatacgtt aacttcagta atagaagcat tcgattgtta
     6961 aatagagtgc ttaaaactgc ataaggcaca ccggcatatt ttccaatact cgttttgccg
     7021 ctactccctg acgctccatt tgcacagttg aataaaaaaa agagagatag aattaaaata
     7081 taaaatgcag atggcaattt atttaatttt tgattcatat ttttcataat aaaaaactaa
     7141 ccataagcgc aaagttaaaa aattcaccat tttttaattg atattgattt ggttgtgaaa
     7201 gtctataatc tctatctaat ggagaaagat atggatattg tgtagcggga tttttatcta
     7261 cccaaaatgt ctcgtagatc acagtgtaat caactctcag accaatacga acctttcttc
     7321 cggcaacaaa acttacttct gttccgagta tagacattgg atcccatctt cctgtatttg
     7381 ctggtcttgc aataacataa gaggctcctc ctcctgcttt taaaaaaatg gaaaccggaa
     7441 gttcaaaggg aagtttgtaa caaagagcag tgtaaattgg tactgtaaat aaacttcttt
     7501 cggtttggga aagatagttt gccattcctg ctccaatttc tgtataaaaa acatacggcc
     7561 aattgaatct tgtaaataaa ccaaagccga gtgttgtatc tagcacggtt gcagtctgag
     7621 atccaggtct tggatttgcc gctcctatcc aaaaaccaat ttctggtttt gcttgttctg
     7681 tttgttgcgc agaaagggga ggaattgagg ataacaatat tattccgctg atgattaaat
     7741 aaaagtattt tatagttcga atgatcggca tagatttatg aataaggaaa ctcaataaag
     7801 gaaagatctt tatgagttag actatctcca cgtttttata aatttgtgaa tataagtgat
     7861 ttacaatcgg ataatcattg taatacgctc tgacaaaata aagtccataa ggaggagcag
     7921 tttttcctgc tttggttcta tctagacttt ctaagatttc taaaacactg gagcgaattt
     7981 ttcctcttgc tatatctaat agagttccta ctagaatccg aaccatattg tgaagaaagc
     8041 ctgttcctcg gattacaatt ttaaatagat ttttcaattc ttcgtcttct ttgatttcaa
     8101 tttttgtaat tcttctttct gtagaagtat atgtttctaa aacagatttt tttgtgaaac
     8161 tggcaaaatc atgttttcct attaaacttt ccatttctct ttgtaagaga ttgaaatcaa
     8221 ttttattttt aaaccaaaga gctcgttctt ttaataaagg atgaggtgtt tgcgtattta
     8281 gaattttata ttcatattct ctttctgtgc atgaaaaacg agcatgaaat ccatcaggaa
     8341 cttcatttgc agtcaaaact gcgagccccc cttctagacc cgcaattgca tttacggata
     8401 gtattagctt cccataattt tggatgggat tttgggatgt aaaactaact gccattcctt
     8461 ttgcatgaac gcctgcatcg gttctacctg ctgctacaag tgtaatattt tctcgtaata
     8521 tttgtgaaag gattgtttct agtttacctt ggactgtaac tctcttagca ctttcttgct
     8581 tttgaaaccc gttgaattga ggaccatcat attctagaat caatacactg cttggcaagc
     8641 ttacttttct cctcctagag attcaataag ctttgctagt tcatcatata attcttttgc
     8701 catatcaaga atagcagacg gtttggattt agaggatttt ccagatccat ataatttggc
     8761 aagcattcgc tttgatttgg ctagaagttt tactctttcc tctggaatat cgctcatcat
     8821 gtctctaaat ttcatggtaa gataagcatt tagataaata acaccatcaa atccccaatt
     8881 gttgtctgta tcgggtccta aaatgggagc cgcagaatca actggctcct gtccggtttg
     8941 cataatgtct aatgtcaatc cataccaaga ggctgctttt tggtagagag tatctcggac
     9001 tttatcgtaa cctaaatttg gaaattcggt atgcaaatca tcaaaatacc aagctgcctt
     9061 caatgcacaa acagcttttt tgggagttgg ggcatccttt ggagatctat tttgataaga
     9121 atcaatcgct aataaaaaag atccagcccc tgtgactaaa tttctttctg cggaaaaatc
     9181 tactggtcca atgatttttt ctaagtttac ctttctgtct cccgattgaa atttcattct
     9241 ctctaaatcg ggtcccgcta gagtgctcca atccttcgga aaagatgcgt ataaacagcg
     9301 tggacatact acaataacat aatcatttgg gttaattcta ccaaattttt tatttttctc
     9361 gtagagacgt cgaagttcct ttgttaactt ccctgcaatg agtcgtcctc ctccctggaa
     9421 catttgctct ctttggtgga cggtatcgca aacaggacaa acactatttt ctttcgaacg
     9481 aaaggaggct ttttttagtg gttcaacttt tgtattagca ggcatttata ttctattcct
     9541 ctcaaataaa cattcattta aatactaata aatgtcaatc tattatactt gaaatccgat
     9601 attagtacgg acaggtttta aactgcttat atcatttccc taaatggtaa atgtttttac
     9661 tatttaaaac aagttggttt tttatacact aaatctttta tagtttctgc atactattaa
     9721 agagtcagta tatataattc tacttgaact tatagggtta ttataagagt ttagtacaag
     9781 cttcatatag actaacaggc ataggacaga caatgaaaaa aatcatcata tctcttacat
     9841 taatattcgg attcagttcg ctatttgcag agccaattat gaccaaagcg tatcaaaaac
     9901 gtgtagaact acttggctac ttacgaattt tagagccaat cgtaaaaaac tatcgtggaa
     9961 acgataaaga gggaaaacca gctttattaa aagcggaagt aggtaaagaa ggggaaagag
    10021 ttagacgtta taacgaaatt aaaaaacttt ttcaagaagg gcttacagat tatttcgaag
    10081 gagattattc aaactcctat agaagatttt tagaagcaca agtagacaca gaacaactct
    10141 tagaagaaat ctctgaattt tatatcgaag gcactgcaga aattttaaag gctgctgttt
    10201 ttgctaaaga tcaaaaagaa tacgaagatt ccgcttttga tgcaaaagag ggaaataaaa
    10261 aagttggtga tagcttaaaa gatgtagata gagagttagt tgatattagc gtagagtatg
    10321 gaagaggttc taaaaacgta ggagagttca gagagaatag agaagctcct tatgtttcta
    10381 gacaatatga tgcaaaagaa tttcattttg ccacaaataa gtatgcaatc gaacaaaata
    10441 cagaagctgg ttacagagct cttggtcaag ctaagaaagc tagaattgaa gctttaaaaa
    10501 ttgaaagaaa tttagaaaga catcaaaaac tacaaccagt tcatagaaag tatagaattg
    10561 aaaaatacct agacgtaatc gctagatgta gagatggtag agaagcagct attaaccttt
    10621 ttagattaaa atatccttat gacaattatt atctccaaaa agatgataaa acaactttgg
    10681 gaaaagtatc tttagctgat gcaaaaggcg atgaggttgc attggacaaa ggaaagtcta
    10741 tgaacttcag actcaatcct catgttctac caaaaaattt aaatcctgta tttgatagaa
    10801 gaattcctga gaaatataga agagatgcaa gtgatcttct tggaaaagta tatgaggaag
    10861 aaatagaaga aagtgttcag ttaaagttta cagatttaaa tcatgtaaac tctgatctca
    10921 aaagacaact catcaatgaa acgttagttg tagaagatgg aaaagattct ccaaaaggag
    10981 attctaaacc tgctgcaact gaaccaaaaa aataatatta aataaaaatc agggatagac
    11041 tctaagttta tccctgcaaa tctaatttac tccttatctc aaaaaacatg gcaaccttaa
    11101 atccttataa atatttaaaa cagaatctgg gggatcaatc ccaagattat cattggttaa
    11161 tcgttagaac ctacatggaa gttctagtag tcatttttac tagagccaaa agaattttat
    11221 taaaaggact tttctactct ttcttgaata aagaaaagaa agcttatgct ttcttagaag
    11281 gttcttggct ctggggcgat agaatgatta aggttactaa aactaaactt gttcttgtga
    11341 atgaaattaa gattccagaa agaaatcata tgatttttgt gaatcatgtc aatgagcttg
    11401 attttccgtt tgattgctat gtgattaaga aaccatatct cgcaaatcag caaattaaga
    11461 gtagctattt cgcctattgg tggatgaaag caatgggatc acaggtgttt gatacatcgc
    11521 aagcaagaac aattgcaaag tcagttcatg aattagtcga agggcttaaa atgtttagtt
    11581 atatcgttta tcctgaaggt cataattctt ataatgaaga aattaaaccc ttgaaaaaag
    11641 gaatggtcaa ggttgccttc gagaataaaa tccctatcct agttattcta aaatctggta
    11701 ttactaagtt tcaaacttct ccaaaaggaa acgttatcgg atataaatcg gctggcattg
    11761 tagatccaac tcaatttaaa acatgggaag agttaaaaga ccatcttttt aaattaatgt
    11821 cagaagaaaa aaaagcattg gatgaatacg tagccaaagc atgaaaaaga ttgagcggct
    11881 actcattgct aaccgcgggg agatagcggt tcgaattatt agaacctgca agaaacttgg
    11941 aatccattct atttgcattt attcagaagc ggatagtgat agtgtatttg ttaagctagc
    12001 cgacgaatct catttagtag gtccggctca ggcaagtgaa tcctatttac acattcccaa
    12061 aattattgat atatgtaaaa aatcaaaagc agatgcagtc catcctggtt atggattttt
    12121 gtccgaaagt cccgcttttg ccaaagcgtt agaagaagca ggtattttat ttataggtcc
    12181 tagtgtagaa tctgttcgat tgatgggtga taaaattcaa tctagaattg caatgattca
    12241 agccgggatt cctgttgttc cgggttatga cggaaaagac caagacggca aattgctatt
    12301 agcccatgca aaagaaattg gttttcctgt tatggtcaaa gcaagtgctg ggggcggtgg
    12361 aaaaggtatg cgtcgagtgg atagagaaga ggactttatg gaaagtcttt tatctgctaa
    12421 aagggaagct aaaaattttt tcggagacga tactatattt atagaaaaat ttatctcaaa
    12481 tccgcgtcac atagagtttc aagtttttgg tgacaaacat ggtaatgcga ttcatgtgtt
    12541 tgaacgagac tgctctgttc aaagaagaca tcaaaaaatt atagaagagt cgcctgcatt
    12601 taatttagat aatgaactcc gaaataaaat gggtgaagtt gccgtgtgtg ttgcaaagtc
    12661 tatttcttat atcggtgctg gaactgtaga atttattgta agtgataaaa atgagtttta
    12721 ttttatggaa atgaatactc gtttgcaagt agagcatcca gtgacagaaa tggtaacgga
    12781 tctagattta gttgagctcc aaattaaaat tgcagaaggc gataaacttt ccgttctacc
    12841 gaagctttta gaaaaacact ctattgaagt gagaatttat gcagaagatc cagaaaataa
    12901 ttttctacct tctacaggaa aaattctttt cttaaaaaat ccgtcaggcg atggagttcg
    12961 tttggattca ggaatagagc ttggatcgga agtgactatc tattatgatc caatgattgc
    13021 aaaactaata gtaacagctc caaatcgatc gatttgtata gaaaatctta tttcggctct
    13081 tgatcaattt ataatctttg gaattactac aaatatatct tatctaaaaa aaatactttc
    13141 tcattctgaa ttcaaagctg gaaatctatc cactggattt atagataagt atttaaaaaa
    13201 tgatatatta attgataatt ctttagtgga tactatcggt tatgctcatt tactttctaa
    13261 ttcaaaagtt caaagccaat ttgtaaatga taactttaga aattttcagt tttgggcaaa
    13321 gaatgctatt tctaataagt tacaaacaat tctatttcct gatattttaa attctatttc
    13381 taaatggaaa tatagaggag aactttttga agttaaagtt ctttcctttt cagaaactga
    13441 ttcaaatttt aaattcagaa tttctatctt gaacttggat acgaagcatt acacagaaag
    13501 agatttagag actgcaaaag aaaaatttgg gaattccatc caaacaaact tgggagaaat
    13561 tattttttat cagtatgggc taacggcatt tatccactta aatggaactg cttatcaatt
    13621 aagacgagaa caaagatctg ttttagcatc ccaaaatcaa tcgaacacat ttacaagtcc
    13681 tatgcccgga aaagtaattc aaatctttgt aaaagaaaaa gatttcgtga aggcaggcga
    13741 tacgcttgct attgtagaag caatgaaaat ggagaacgcg attaaatctc ataaagactg
    13801 tgaggttgta gaggtttcct gcaatattgg ggatttagtt agacaggatg atattctgat
    13861 tcgagtcaag tagtttaaaa tgagcaattt ctaaattgaa ctgaaaatct gttccatttt
    13921 aaacattctg attgtatgga atcacgagaa attgcggatt ttgctctcaa atacagtcaa
    13981 aagtatccaa ttcttattat atccggacct agacaatcgg gcaaaacaac cttaagtaag
    14041 ttactttttc ctaaataaaa tatgtgactc tggaaaatcc agatgaaaga atctttgatc
    14101 aaaaactata tctaatagat atgtatgctt tttctactga catatcttga aaaagatgag
    14161 agaaatgttt tgaatagtaa agatttatct caatttgaaa ttttttaaaa acttacatta
    14221 ttacgaaaaa caagatttac agattcaaaa aaaatattta gtatatggag gaagtataaa
    14281 cagaattcaa tcggatgtag aagttgtttc ttgggaaaag gctggaaatg ttggtcttta
    14341 ctaattctaa catgaactcg atgtaaaaat cttttcaaca gctataacta aacgatgaag
    14401 gcaggattga taattttgaa tgaagatagc ttttatcagt tatagtcgta tctattggtg
    14461 gtaatctttt tttttattag atcgaactag cgttaaatct atttcttatc gctatctaac
    14521 tctaccaaga cttggtctat aatgtctgct aattgaacta attgtttttc tgataagtgt
    14581 gagatgagtt gtttgccaaa tgtatttaat actccaaggc ctgcatttac tcccatattg
    14641 actactcctc tgctgagtgg accaagattc aaaatttcca aaggtcgaac aaaataatag
    14701 tgaagacttt cttctgtaat attgtaaatt gcgcgtaacg ctttctccgg catgtccttc
    14761 tcatgcggtt tttcacgaag catctgtgta gcttctcttg tcttttttgc ggtcgttaga
    14821 gaaactgggc tcattaaata gaattttttt tcatgcgaat cattcatagt attcccttta
    14881 tttgctgaag ctcgactacc agtatctgat taaggctgaa gttcattcaa actatttatg
    14941 gaagatggta tggtttttta ggagtgtttt gaagagtaga aaaattacta tagaattttt
    15001 ctttgtctcc ttctctaaat ccagtaataa gtagtcctag aatcatatca tcattttgat
    15061 ctctaccaat aaatcgtatt tcacattgta tattaattgc ggactgcatt ttaaagaaaa
    15121 tatcacaatt aaaacctgtc acatgcggta aatgttgttt tagttctcga tctggcactc
    15181 ttatttttaa tccacttttt gagagatcta agattctaaa tttattcgta tttaagataa
    15241 agttagattc acgaattcga tcaatcattt caaaggtgag aattttaagc tccatcactt
    15301 gatcatgatc gattggtttt gttcgacttt ggatatgcac ataaccaaat ggaattgcta
    15361 cattctgagt gttaacgtag atgactggca ttataatctc agatactatg ttttttgttt
    15421 tatacatgct gatcaatcga ttgatttctg catgatactc ttcttccaag tcaataaagt
    15481 catcatccgt aaaggcggtg tagcactttc gatcctgtgt attggcaaca tacaatgttt
    15541 tacctgattc ttttacaacg tgaaatcttt cttcaagatc tggcttgaag atatcaattt
    15601 ttatataatc
//
LOCUS       DAOQOS010000124        15399 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_1942_length_15399_cov_6.082769, whole genome shotgun sequence.
ACCESSION   DAOQOS010000124 DAOQOS010000000
VERSION     DAOQOS010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 15399)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15399)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15399
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1942_length_15399_cov_6.082769"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1122)
                     /locus_tag="PLF66_20710"
     CDS             complement(<1..1122)
                     /locus_tag="PLF66_20710"
                     /inference="COORDINATES: protein motif:HMM:NF026253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HMZ66525.1"
                     /translation="MKFLILVAPLFFSSCISLFIDSLNEPETKYSVGFINEEGNLIIP
                     YGYEEAGEFVEGLAPVKKSGLWGFINKKGEVVIDFQFKDAKPFSTDPLWAPVEKNGIG
                     EKAWGYIDKKGNWMIEPSFYNATVFRNGVAEVATTKFKFKQVSKRYDKYFLTKDKKYI
                     YHNGLYSYLAGPGRYSEGMLPSCKDGKWGFKDVNDKWIVEPKYTIVRNFSDGYAAVQI
                     MNPNPYTDCELISESEPFGLWGYIDKTGKEIIPPKYRMARDFVNGIASVDETFQEKDM
                     IPGNTFSRYFIDKTGKKLFDTSYAQTENFLDSGYAFVGQDEKNLLQLDPNDSIFIDTK
                     GKKAPFQLEKNERIFTVRKSGKDFLLITFQVRPNPERYEH"
     gene            1534..1668
                     /locus_tag="PLF66_20715"
     CDS             1534..1668
                     /locus_tag="PLF66_20715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66526.1"
                     /translation="MQLIQSSLETPKNKNPRKMDFFKLESKELSALKLLSSKQTAMKL
                     "
     gene            1948..3612
                     /locus_tag="PLF66_20720"
     CDS             1948..3612
                     /locus_tag="PLF66_20720"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016560968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HMZ66527.1"
                     /translation="MATIASEKETALKALAASGKLIEDITKGLATQCTVKGAISVSKM
                     DENQYVFYQLSWMTAEQKIAEHFINYAWDSSFGTGELEQEMAIVFAAEVVSHIRSELI
                     SKPSEYTTTHEKVTSELFNSTVNQFIEASTKIDNYSKIIDTINKVGHAGAYGLGDDHE
                     SFRETFRKFAEDVVKPHAEHVHRSDDIIPQDIIDGLNDMGCFGLCIPETYGGIQPADK
                     PDNISMLVVTEELSRGGLGIAGSLITRPEIVSKAILKGGTDAQKEKWLGKLASGEVMA
                     AVAVTEPNYGSDVAGIIVTGTKVEGGWKINGVKTWCTFAGYANVLLVLCRTESDLDLK
                     HKGLSILLAEKPRFLGHSFEHKQEGGGRIEGKAIGTIGYRGMHSFEVSFEDYFVPDEN
                     LVGGEAGRGKGFYLQMEGFAGGRIQTAARANGVMQAAFEAGLRYATERQVFKKPIYEY
                     NLTKYKLARMAMIIQASRQFTNFVARLMDEHKGQMEATLIKFYASKISEWVCREAMQI
                     HGGMGYAEEYAVSRYFVDSRVFSIFEGAEEVMALRVIAKGLLEAKV"
     gene            3658..4026
                     /locus_tag="PLF66_20725"
     CDS             3658..4026
                     /locus_tag="PLF66_20725"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HMZ66528.1"
                     /translation="MFGLFSESILETMKIDLDLKPPFAIFHFSGKLAFTELASIRAKL
                     NPILREAKHKIVFDLKELESIDSSGVALLFHVAQEATNYKDGSFSLTAPGDFVKEVMV
                     LSGIYNKFIIHPTVDEALRN"
     gene            4263..4664
                     /locus_tag="PLF66_20730"
     CDS             4263..4664
                     /locus_tag="PLF66_20730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66529.1"
                     /translation="MFSGIISFFKKNPKTLFLIDAMGALITTLILFIIQSFFLSYFGF
                     LKTVFQYLILLGLLLFLCSSSCTFFLKEKFAFFLRGIAFGNLFYCCLTFVIIYFYYSI
                     LSPIGIIYFASEIATISILVFIELQVARGLQ"
     gene            complement(4670..7774)
                     /locus_tag="PLF66_20735"
     CDS             complement(4670..7774)
                     /locus_tag="PLF66_20735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002619630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HMZ66530.1"
                     /translation="MTLSDISIKNPVFAWMLMFGLIFFGFISFRKMGLSQMPDVDFPV
                     VNIAVNLNGAAPEIMETNVVDIIEESVMSVEGIISISSTSRYGSANVTVEFDLNRDVD
                     AALQEVQTKVIQAQKLLPKELDPPIVTKYNPEEQPIMWVGVSSTGNKRDLMIYVKDHL
                     KNSFSTVPGVGQVMLGGYTDRNLRIWVDGKKLSQRELTIDDIIGTIQKEHIEVPAGLI
                     ETQTQQLNIRSLGEAPSVSVFGNLPISYRGGAPIFDSNIRIRDVARLEDGLDDVLRAS
                     RFNGKQAVGLGIIKQRGVNAVQVAADIRKQIEAVKKDLPPGFEIGVNSDSTKFIKESI
                     EELEFTLILSAILTSLVTWLFLGSLSSTINILLAIPTSIMGSFIVLNYFGFTLNTFTL
                     LGLSLAIGIVVDDAIMVLENIVRHREMGESLFNAAIKGAREITFAAMAATLAIVAIFL
                     PVAFMTGVIGRYFYQFAITITAAVLISLLEALTLTPMRCSQFLQVRSESKLMNWIEKG
                     FLSLEKFYERVLHFCLNHKLIVIILSLLIFVGSIFIIKPIKKEFVPSQDQSQLLVRMQ
                     TPVGSSFSYTDSMATKCEEYLLKNPNVERVYVTIGGFGGMGAQANIAMFFVTLKDPSK
                     RTKNPETKKTPTQNEIAGVFRKELGKVSKDLKVVVQDLSMRGFASSRGFPVEFYIQGR
                     NWEKLAESSDLIIKQMKESGNFVDVDTNYQIGQPEVHIVPNREAAMRYGVSVASIGTV
                     IGAMIGGIKSGQFTESGHRYYVNIRVEAGERQKIDDIKNLFIRNNKGELVRLSEVVKI
                     EEKSALQSITRISRERAITIFANPAPGTSQEIALKKAEEIARKTLPEGYSVAFSGSAK
                     TMGDSFNSLFYALMLGIVIAYMILASQFNSYIHPVTVLLALPFSFTGAVFSLWVTGNS
                     LNVYSFIALILLMGLVKKNSIILVDFTNQLRVRGLDVREAILQACPVRLRPILMTSIS
                     TIAAAIPPALSIGPGGESRVPMAIAIIGGILVSTLLTLVVVPCAYLVFARIERNKYQL
                     EEIE"
     gene            complement(7771..9288)
                     /locus_tag="PLF66_20740"
     CDS             complement(7771..9288)
                     /locus_tag="PLF66_20740"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HMZ66531.1"
                     /translation="MMLQINLTKILFFVLFLFIYCSHEEQIKDTEVEKDKAENVENIS
                     TPNERIKPVSQPKETYRLEDLYFAAITQTERIAIKKEAIIQAEAKRDSFFANFFPSLA
                     FRYQQFVTIPNHAEHDREVRNRNGLIDAYSNLVYGTRIATPYDIGNSSFGSSSSSTAT
                     SPLVRPGARLVLHIPIMTGLNEWATYKSSKHEVKMRNLELKYESGRMFLEIAQAYYNL
                     LQLESNLQSKKQVLELTKESRIELERRVRLGRNKPSELTSITAQVSKLEGEILGIEDT
                     VSQMRDTLAFLTGMEKEFKIEKVNESPIAIELEEAEKTIESRYDVNAAKLNLEIAKSE
                     VIKAYGGHLPTASVDTFYTFPSGHVNGSTKDLVNQFILQVPILSLGTIQAAVKQAESV
                     KRQAELQLTQSIRLAREEIRKAYNSYIHSSKAEESYLKALSATESNYKVVERDYYKKA
                     ATSLDLLNAQIALKNAKEDLYRTILQKQLNLIWLKVAIGKYPEDIGNKVEERRIP"
     gene            10193..12307
                     /locus_tag="PLF66_20745"
     CDS             10193..12307
                     /locus_tag="PLF66_20745"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HMZ66532.1"
                     /translation="MIYKIVLINIILSFFSCSIQEKPKEPLAEKGVLDLRQWDFEKYG
                     KLDLYGDWEFYYGEFLLREDFKKNTRENPHLIPIPSLWNGYDWNGSKLPGFGYATYRL
                     TVKVDPKYLNQIMGLRIGDMYSSYRLFVNGKLIASNGKPGKIKEETVAQWLPVVRFIN
                     IESEEIEFILHIANYNHRKAGTWAFLEFGNPDEIVTARERAMAFELFLFGSLIIMSFY
                     HFGLYALRKNNRSVLYFGIFCFLTGFRVMVTGERSLVNFFPLSFEGQLKIEYLSLSLG
                     IPAFSHFLIHSFPEIVNRKAFYIPDFFLILFSIIPLFTTGEIYSYTAIPVQLNMLLVA
                     ILFFYYLIKANRLGKDGAFSAILGMIIFFICGVNDALYNNELIQTGNLYPFGLFIFIF
                     SQSFILSLLFSKSFRNVENLSIELTETNRAYTRFVPREFLNFLNKKSILDIQLGDQVQ
                     KEMTILFSDIRSFTTLSEQMSPEENFNFINSYLSRMNPIIKQNNGFIDKYIGDAIMAL
                     FASTPADAIRAAIHMQQEILKYNRDRVDHNFPIIKIGVGLHSGKLMLGTIGGDDRMEG
                     TVISDAVNLAARLEGLTKVYGSLILTSEETFLKANGKENFKYRRLGKARVKGKQDSVV
                     IIDILDGQSEFWLDVYLQTKEDFEKALDLYESKDFKKAGELFDKVLDVNPSDVAARFL
                     YQKSGYYETHGTPEDWDGTESV"
     gene            12383..13054
                     /locus_tag="PLF66_20750"
     CDS             12383..13054
                     /locus_tag="PLF66_20750"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HMZ66533.1"
                     /translation="MNQKFLIGVSVGILFTPLYSEILYLQKPKVQEEVAFFSMQPAKD
                     PNKTNVSSQTQNTQPVLSSQPKQFYSEFIPKPNEPVPEKDKLVIPLTPPSDPPRFIPN
                     PHPQFGKPFPHKFIARVNFSANPENPHKGVLLTPIESNKVLATLEGKVVAIDYMDGYN
                     NYIILEHSNGYFTVYGNLDTVFVAEGQKVKKGDHLGNLLKEKGLYFQVNQGKKILDPI
                     SFIKS"
     gene            13116..14174
                     /locus_tag="PLF66_20755"
     CDS             13116..14174
                     /locus_tag="PLF66_20755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012388147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HMZ66534.1"
                     /translation="MAGPIIRNYKAGSIVYFEKDRAEDIYVLQSGRVILTYTSVDSKM
                     EIKEDVKLGEFFGVKSALGRYPREETAQVIGGASILVFKLADFEVFVANKTHLILKMM
                     KVFSSQLRQIHGKVRENLGQFGDQKSPAFELMNVAEVFHKTGNYEHAVYAYNKYLEHY
                     PSGPYVKRAQDLVKIAAKGGMFPPNVPELVYEPERRGDFNRPATTSNPNPAATVTRIQ
                     ALSTDLSISDSIDDADEKYESGNFQGALDTYKAIITMPPAAPGEDKLIEKAHFYFGMS
                     QMKLQLWDLAYASLSNYVKKYPKGEKVKEAIYNLGAISESKGEKEKAVMLYKKVASLP
                     PADEYTSKANSEIARLGG"
     gene            14176..14808
                     /locus_tag="PLF66_20760"
     CDS             14176..14808
                     /locus_tag="PLF66_20760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015681348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HMZ66535.1"
                     /translation="MLEAMFGKFGKVFKPNEIIFCEYEPGNDFYLIQEGQVKITKTVG
                     SSIKTLDILEAGDIFGEMAILEEQPRSATAVCITEVRALNFNRANFELLMTKNPQLAL
                     RVLTIFSTRIYDAKRRLMILLMDDIIGKVADVFLMLYEKQGANEHQKEVTLKVTVDDV
                     GHWCGQPVGEVQKVLAQFKKSGKIDIYNDRVVVHNIQEFQRIIAQKRKEK"
     gene            14825..14898
                     /locus_tag="PLF66_20765"
     tRNA            14825..14898
                     /locus_tag="PLF66_20765"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14859..14861,aa:Arg,seq:cct)
BASE COUNT         4908 a         2856 c         2689 g         4946 t
ORIGIN      
        1 atgctcataa cgttctggat tgggtctaac ttgaaacgtg ataagtaaaa aatcctttcc
       61 actttttctt accgtaaaga ttctttcatt tttctctagt tggaagggag cctttttgcc
      121 ttttgtatca ataaatatag aatcgtttgg atccaattgc agtaaattct tttcatcttg
      181 acctacgaac gcatatccag aatccaaaaa attttctgtt tgagcataac ttgtatcaaa
      241 tagttttttg cctgttttat caataaaata ccgactaaac gtatttcctg gaatcatatc
      301 tttctcttgg aaggtttcat ctacagatgc aatcccattc acaaaatctc ttgccattct
      361 atatttagga ggaatgattt cttttcctgt tttatcaata tatccccaaa gcccaaaggg
      421 ttcggattca gagataagtt cacaatctgt ataaggattt ggattcatga tttgcactgc
      481 tgcatatcca tctgaaaaat ttctaacaat tgtatacttt ggttctacaa tccatttatc
      541 attcacatct ttaaaacccc actttccatc tttacaggat gggagcattc cttccgaata
      601 acgtcctggt ccagccaaat aagaatacag tccattgtga tagatatatt ttttatcttt
      661 tgttagaaag tatttgtcat aacgtttaga aacttgcttg aacttgaatt ttgtagtggc
      721 gacttctgcc actccatttc taaatacagt tgcgttgtaa aaggatggtt cgatcatcca
      781 attccctttt ttatcaatat aaccccaagc cttttctcca attccatttt tctcaacggg
      841 tgcccaaagg ggatcagtag aaaaaggttt cgcatctttg aattgaaaat cgataaccac
      901 ttctcctttt ttatttataa atccccagag tcctgatttt tttacgggag ccaatccttc
      961 tacaaattct cccgcttctt cataaccata tgggattatc aaattaccct cttcatttat
     1021 aaatcctacc gaatattttg tttctggttc gtttaaagaa tctatgaaaa gagatataca
     1081 ggaggaaaaa aatagcggtg caacaagaat taaaaatttc atagtttgaa taaaataaat
     1141 tttagaagat gagtcaagaa gatataatga aataaatttg aatttgtcta attctgcatt
     1201 gaggcaaatt tcaataagcc gtcatgataa aagtgctttg ctagcgttag ggatcgaacg
     1261 gtgtcctttt ttttatccgt tcaaaaaaag aggacaatag ggagatcccg acgcgcagtg
     1321 ctgtcaactt aagagaaatc aaaaagaatt aaccattaag agcacagaga acacgaagaa
     1381 aagagaagat ttattattta ttcacttcgt gagtcttcgt gcattcccgc tcatcgcttg
     1441 acgctcacta tagccaagct atgtggttaa aaatccttaa gttaaaagta tatccgacgc
     1501 gagaacagta agaaatatac atagaatgta aatatgcagc taatacaaag tagcctcgaa
     1561 acacccaaaa acaaaaatcc tagaaaaatg gatttcttta aactagaatc caaagaactg
     1621 agtgccttaa aactactatc ttccaaacaa acagcaatga aattgtaatg aaaacatcca
     1681 cagccgagtg ataaaaaaaa gtaggcattc ataaattatc gtatctatta tgctgtttat
     1741 cggtgttcat ccgtggtaat cttttccctt ccctaaatta caaactttca gactacataa
     1801 gtagaccatc cccttttact tcggtttaca ctaaatccaa ctaaaaagcc tgaattttgt
     1861 tcccgaaaag ccccttaatt tcgaagtgac aagaaagtga aatcataagt tattgtaagt
     1921 aacattaaac aatgaaggat ttttccaatg gctacaatcg catcagaaaa agaaacagcc
     1981 cttaaagcac ttgccgcatc tggcaaacta attgaggaca ttacaaaagg acttgctacc
     2041 caatgcacgg taaaaggtgc aatttctgta agtaagatgg atgaaaacca atatgtattc
     2101 tatcaactct cgtggatgac cgcagaacaa aagattgctg agcactttat caattatgct
     2161 tgggattcct cctttggaac tggtgagcta gaacaagaaa tggcaattgt atttgccgca
     2221 gaagttgtat cccatattcg atctgaatta atatccaaac cctcagaata tacaacaact
     2281 catgaaaaag tgacttctga acttttcaac tcaacagtaa accaattcat cgaagcttca
     2341 actaaaattg ataactactc caaaatcata gatacaatca ataaggttgg acatgctgga
     2401 gcgtatggct taggagatga ccatgaatct ttccgcgaaa cattccgtaa gttcgcagaa
     2461 gatgtagtga agcctcatgc agaacacgtg catcgttctg atgatatcat tcctcaagac
     2521 atcattgatg gacttaatga tatgggttgt ttcggtcttt gtatcccaga aacctatgga
     2581 ggaattcaac ctgcagataa gcctgataat atcagtatgc ttgttgttac agaagaatta
     2641 tcccgtggcg gtttaggaat tgcaggtagt ttaatcactc gaccagaaat cgtttctaag
     2701 gcaatcctca aaggtggaac tgacgcacaa aaggaaaaat ggctcggtaa attggcaagt
     2761 ggcgaggtta tggcggctgt cgctgtaaca gaaccaaatt atggttctga tgttgctggt
     2821 ataatcgtta ctggaaccaa agtagaaggc ggatggaaaa tcaatggtgt taaaacttgg
     2881 tgcacatttg caggatatgc aaatgttcta ctcgttcttt gccgcacaga atccgatcta
     2941 gatttaaaac acaaaggtct atctatccta ctcgcagaaa agccaagatt cctaggacat
     3001 agcttcgaac acaaacaaga aggtggcgga agaatcgaag gaaaagcgat tggaactatc
     3061 ggttaccgtg gaatgcactc ttttgaagta tccttcgaag attattttgt tccagatgaa
     3121 aacctagttg ggggagaggc tggaagagga aaaggattct acttacaaat ggaaggtttt
     3181 gctggtggtc gtatccaaac agctgctcgt gcgaatggtg ttatgcaagc tgcatttgaa
     3241 gcaggtctac gatacgcgac agaacgccaa gttttcaaaa aaccaatcta tgaatacaac
     3301 cttacaaaat acaaacttgc tagaatggca atgatcattc aagcatctcg ccagtttaca
     3361 aactttgtag caagactcat ggacgagcat aaaggacaaa tggaagcaac tcttattaag
     3421 ttctatgctt ctaaaatttc tgaatgggtt tgtcgtgaag caatgcagat tcacggtggt
     3481 atgggttatg cggaagagta tgccgtatct cgctatttcg tagattcaag agtattctct
     3541 atctttgaag gcgcggaaga agtgatggct cttcgtgtaa ttgcaaaagg tctcttggaa
     3601 gcaaaggttt aagggaaatc tttgagggct cttcaataaa aatacttgac tcagagaatg
     3661 tttggattat tctctgagtc cattttagaa accatgaaaa ttgacctaga cttaaagccc
     3721 cctttcgcaa tcttccactt tagtggtaaa ctcgcattta ctgaacttgc atccattcga
     3781 gcaaaattaa atccaatctt aagagaagcc aaacataaaa tcgtatttga tctaaaggaa
     3841 ttagaatcga tcgactcttc tggtgtagca ctcttatttc acgtagcaca agaagcaacc
     3901 aattacaaag atgggtcttt ttccctcact gctcctggtg attttgtaaa agaggtaatg
     3961 gttttatctg gaatttacaa taaatttatc atacatccta cagtcgacga agcacttcga
     4021 aattgagatt tcatataaca ctgatttgct gcttgtaaaa ttggctcttt tcgctgactc
     4081 gcaaatagca tcctttggtt ttataaaata attcccgaat catatttcca taatccattt
     4141 taggttcaaa tccttttaaa gaattttata ccgatttgga ttttgtataa tctaataaag
     4201 agtaatgaat aaaagtaaaa ctttttaaaa agctattcta tcaaattaag aggataaatc
     4261 atatgttttc aggaatcatc tcctttttta aaaaaaatcc aaagactctt ttcttaatag
     4321 atgctatggg agcattgatc acaactttaa ttctatttat aattcaaagt ttttttcttt
     4381 cttactttgg atttttgaaa acagttttcc aatatctaat cttacttggt ttgcttttat
     4441 ttctctgttc ttcttcttgc acattctttt taaaagagaa atttgctttc tttttgagag
     4501 gaattgcatt tggaaatcta ttctactgtt gcctcacatt cgtgattatc tatttctact
     4561 attcaattct atcaccaatt ggaatcattt attttgcgag cgagatcgca accatttcga
     4621 ttcttgtctt tatagaatta caggttgcga gaggtcttca gtaagaaccc tactcgatct
     4681 cttccaactg atacttgttt ctttcaattc gggcaaatac taagtaggcg caaggaacta
     4741 caaccaaagt aagaagagtt gaaaccaaaa ttccacctat gatcgcaatt gccataggga
     4801 ctctggattc tcctcctggt ccaatcgaga gtgctggtgg gatcgctgct gcgatagtcg
     4861 aaatagaagt cataagaatg ggtctaagtc gaaccggaca ggcttgtaaa attgcttctc
     4921 ttacatctag tcctcttacc ctcaactgat ttgtaaaatc gactagtata atagaattct
     4981 tttttacaag tcccatgaga aggattaaag caataaaact ataaacgtta agcgaatttc
     5041 ccgttaccca taaagaaaaa acagctcctg taaaactaaa tggaagagca agtaagactg
     5101 taacaggatg aatataacta ttaaactggc ttgcaagaat catataggca attacaattc
     5161 caagcataag agcataaaac aaactattaa aagaatctcc catggtcttt gcagaaccac
     5221 tgaatgcaac agaataacct tctggtaaag tttttcgagc aatttcttct gcttttttca
     5281 aagcaatctc ttgacttgtt ccgggtgctg gatttgcaaa aattgtaatg gctctttctc
     5341 ggctaatacg tgttatactc tgaagagcag atttttcctc tatcttcaca acttctgata
     5401 gacgaaccag ttctcctttg ttgtttcgaa taaatagatt tttaatatcg tcaatttttt
     5461 gtctctcccc cgcttcgact cgaatattta cataatatct atgtccactc tcagtaaatt
     5521 gtcctgactt aattcctcca atcatcgctc ctatcacagt tccaatgctt gcaacgctca
     5581 ctccataacg cattgctgct tccctgttcg gaacgatatg cacttcgggt tgaccaattt
     5641 gatagtttgt atctacatct acaaaatttc cactctcttt catttgtttt ataattaaat
     5701 cagaactttc agctaatttt tcccaatttc ttccttgtat atagaactca actggaaacc
     5761 cgcgagaaga agcaaagcct ctcattgata aatcttgcac tactactttc aagtccttag
     5821 aaacttttcc gagttctttt ctaaatacgc ctgcaatttc attttgtgtt ggagtctttt
     5881 ttgtctctgg attttttgtt ctcttggaag gatcttttag agtcacaaaa aacatagcaa
     5941 tatttgcttg tgctcccatc cccccaaacc ctcctatggt tacatagacc ctctctacat
     6001 tcggattttt caaaagatat tcttcgcact tcgtagccat tgaatctgta taactaaaag
     6061 aagaccctac tggagtttgc attcgaacga gtaattggct ttggtcttgt gatggaacaa
     6121 attctttttt aattggctta atgataaaaa tagaacctac aaagattaaa agggagagga
     6181 taatcacgat tagcttatga tttaagcaga aatgtaatac tctttcatag aatttttcga
     6241 gtgatagaaa tcctttttcg atccaattca tgagcttact ctcacttctc acctgtaaaa
     6301 actgcgagca tctcattggt gtaagagtta acgcttccaa gagagaaatt aatactgccg
     6361 cagtaatcgt aattgcaaat tggtaaaaat atctcccaat cacaccagtc ataaacgcaa
     6421 ctggtaaaaa aatagctaca atagccagag tcgccgccat cgcagcaaag gtaatttctc
     6481 tcgctccttt gattgctgca ttgaatagtg attcacccat ctctctatgg cggacaatat
     6541 tttcgagaac catgattgca tcatctacta caattccaat tgcaagtgag agtccaagaa
     6601 gtgtaaatgt atttaaagta aagccaaagt aatttaatac aataaaactt cccatgatag
     6661 aagtaggaat tgcaagtagt atattaatcg tagaagagag acttcctaaa aaaagccaag
     6721 tcacaagact tgtaagaatc gcagagagaa tcaaagtaaa ttccaattct tcaatcgatt
     6781 ctttaatgaa tttagtagaa tctgaattga ctccaatctc aaaccctgga ggcaaatcct
     6841 tcttaaccgc ttcgatttgt tttcgaatat ctgctgctac ttggactgca tttactcctc
     6901 tttgtttgat gataccaagt cccactgctt gctttccatt gaaacgagaa gctctgagca
     6961 catcgtctaa accatcttct aaacgagcta catctcgaat ccgaatgtta gaatcaaaaa
     7021 tcggagcacc accacgataa gaaattggta aatttccaaa gacagaaaca gagggagctt
     7081 ccccaagaga acgaatattt aactgctgtg tctgcgtttc tataagtcca gcaggaactt
     7141 cgatatgctc tttttgaatc gttccgatta tatcgtctat cgtcaattct cgttggctca
     7201 gtttttttcc atctacccaa attcgaagat ttcgatctgt atagccccca agcatcacct
     7261 gccccactcc gggaactgta gagaaactat tttttaaatg atctttcaca taaatcatta
     7321 gatctctttt atttcccgta gaagatactc ctacccacat aatcggttgt tcttctggat
     7381 tgtattttgt tacaatcggt ggatctaatt ctttcggaag tagcttttgt gcttggataa
     7441 ctttggtctg cacttcttgt aaagcagcat caacgtctct gtttaaatca aactctacgg
     7501 tcacattggc agaaccataa cgggaggtag agctaatcga tattatcccc tctacactca
     7561 taacactttc ttcgatgatg tccactacgt tcgtctccat gatttcaggc gctgccccat
     7621 ttagatttac tgcgatatta acaactggaa aatctacatc tggcatttgg gaaagtccca
     7681 tcttacgaaa ggaaataaat ccaaagaaaa tcaaaccaaa cataagcatc caagcaaaaa
     7741 ctggattttt aatagatata tcagaaagtg tcatggaatt ctcctttctt ctactttatt
     7801 cccaatgtct tcggggtatt tgcctatcgc aacttttaac caaataagat tgagttgctt
     7861 ttgtaaaata gttcgataca aatcttcctt tgcatttttt agagcaattt gtgcattgag
     7921 caaatctaga ctagttgctg cttttttata gtaatctctc tctactactt tgtaatttga
     7981 ctcggttgca ctgagagcct ttaaataact ttcttctgct ttagatgaat gaatataact
     8041 attgtaagcc tttcgaattt cttctctggc taagcggata gattgtgtta gctgaagttc
     8101 tgcctgcctc ttgactgact cagcttgttt gactgccgct tgaatagttc cgagggatag
     8161 aattggaact tgtaaaataa attggtttac taaatccttt gtgcttccat ttacatgacc
     8221 tgaaggaaat gtatagaatg tatcgactga cgcagttggc aagtgccctc cataggcttt
     8281 gatgacttca gactttgcaa tttctaaatt taatttagct gcattcacgt catatctact
     8341 ttcgattgtc ttttcagctt cttctaactc aatcgcaatg ggagattcat ttactttttc
     8401 aattttaaat tctttttcca ttccagtcaa gaaagccaac gtgtctctca tttgagatac
     8461 agtgtcttcg attcctaaaa tttctccttc tagctttgat acttgagccg ttatgctagt
     8521 aagttcggat ggtttatttc ttccaagacg aactcttctt tctaattcaa ttctagattc
     8581 ttttgttaat tctaatactt gctttttact ttgcaaattg ctttctagtt gtagcaaatt
     8641 atagtaagct tgtgcaattt ctaaaaacat tctacccgat tcatatttta attctaaatt
     8701 tcgcatctta acttcatgtt tggaactttt ataagtagcc cattcattca gtccagtcat
     8761 gatcggaata tgaagcacaa gcctcgcacc aggacgaacc aagggcgagg tcgctgttga
     8821 actactgcta cttccaaaag agctatttcc tatatcgtat ggcgtagcaa ttcgagttcc
     8881 ataaactaaa ttgctatatg catcgattaa cccatttcga ttccgaacct ctctatcgtg
     8941 ttcggcgtga ttgggaatgg ttacaaattg ttgatatcga aaagcaaggg atgggaaaaa
     9001 gtttgcaaag aaagaatctc ttttggcttc tgcttgaatg attgcctctt tcttaattgc
     9061 aattctttct gtttgagtta tcgcagcaaa atacaaatct tctaaccgat aggtttcttt
     9121 tggctggcta acaggcttta tcctctcatt cggagtagaa atattttcta cattctcagc
     9181 tttgtccttc tctacttctg tatctttaat ttgctcttca tgagaacaat aaatgaatag
     9241 gaataaaaca aagaatagaa tcttagttaa attgatttga agcatcataa aaaatttttc
     9301 tccctttcca ttttgtattc tccatacttt tattatgata ttgaaaagaa agattttatt
     9361 caatcttact aaatcatctt acgatttgct tcaatcatta cataattcaa gaaatgagtc
     9421 tgtttagttt agcaaagcaa ctcaataggt ttaattttat ctcatttgaa aaatgggaaa
     9481 agtcttgaaa cgatgctctg ctattttcaa aattaatttt ttatttcaat ttagtagctt
     9541 tgtctaagga tcttgctaat atcttaaatc catacaaaaa ggatttctag tataaagaaa
     9601 caatttctgt ttattttgta ttcttttcat ttgacgcaag cggagtctaa ttttcaattt
     9661 gcttcgaaga tcccaatatt ttgttttaat ttgaaaactc ttcctaatcc tctcaagtct
     9721 gattcattta aacttcggga ttgcctccaa ttcaggttgc ccaataggct aaaagtcttc
     9781 tttctagctt acattctttt aggggaatta gcattatctc tattgaaaca ttgaatagaa
     9841 ggctagatgc ccacttatct tttatttttg gaattatttt cctttctgta aaaactaaat
     9901 ataaaattcg attggcaaat gtattgattt gctttttatt tctatttaaa gaattacgtt
     9961 acaatctaat tcaagtgggt ttgatataaa aaagaaacgg attttcaaaa gttttggttt
    10021 gtaaaaagaa aaggcaggaa atcattttta ggttgtctag cttttagatc atgcttgaga
    10081 ataaactaga gtaagtattt tttaggagtt gagaaatccc ctccaaaata ttttttctat
    10141 ttaactcaca ggaattaaca aaatataaaa ataaaatata aataaacaag acatgattta
    10201 caaaatagtt ttaataaata taatccttag ttttttttcc tgttccattc aagaaaaacc
    10261 gaaagagcca ttggcagaga agggagttct agatttaaga caatgggatt ttgaaaaata
    10321 tggcaaacta gatttatacg gcgattggga attttactat ggagaattcc ttttacgaga
    10381 ggattttaag aaaaacacaa gagaaaatcc acatcttatt ccgattccat ccctttggaa
    10441 tggttatgat tggaatggaa gcaagctacc tggattcggt tatgccacct atcgtctaac
    10501 ggtaaaagta gatccaaaat atctaaatca aatcatggga cttcgtatcg gtgatatgta
    10561 ttcctcttat agattatttg taaatgggaa actaattgct tcaaatggga aaccaggaaa
    10621 aataaaagaa gagacagttg cccagtggtt acctgttgtt cgttttataa acattgaatc
    10681 agaagagatc gaatttattt tacatattgc gaactacaac cacagaaagg cgggaacttg
    10741 ggcatttctt gagtttggca atccagatga aatcgtgacg gcacgcgaaa gagctatggc
    10801 atttgaatta tttttatttg gtagtttaat catcatgtct ttttaccatt ttggtttata
    10861 tgcacttaga aaaaacaacc gatccgttct ctattttgga atcttttgtt ttttgacagg
    10921 atttagagtg atggtaacag gagaaagaag tcttgtaaat tttttccctc tctcattcga
    10981 gggacagcta aaaattgagt atctttcatt gtctttaggg attcccgcct tctctcattt
    11041 tctaattcat tctttcccag aaattgtgaa tcgaaaagca ttttatatac ccgacttctt
    11101 tttaatttta tttagtatta ttcctctttt tacaacagga gagatttaca gctacacagc
    11161 aattcctgtt caattaaata tgctactagt tgccatttta tttttttatt atttaatcaa
    11221 ggcgaatcgt ttaggaaaag atggagcgtt ctccgccata ttaggtatga ttatcttttt
    11281 tatatgtggt gtaaatgatg cattgtataa caatgaactg attcaaactg gaaatcttta
    11341 tccgtttgga ctttttattt ttattttttc ccaatcattt attctatctc ttttgttttc
    11401 taaatctttt cgcaatgtgg aaaatctttc gattgaactc actgagacaa atcgagcgta
    11461 tactcgcttc gttccaagag aatttttaaa ttttctaaac aagaaaagta tcctagatat
    11521 tcaactcggc gaccaggtgc aaaaggaaat gacgattctt ttttctgata ttcgttcttt
    11581 cacaactctc tcagaacaaa tgagtccaga ggaaaatttt aattttatca attcttattt
    11641 aagtcgaatg aatccaatta tcaaacaaaa taatggtttc atagataaat acatcggaga
    11701 tgctatcatg gcactatttg catcaactcc agcggatgca attcgtgcag ctattcatat
    11761 gcaacaggaa attttaaaat acaaccgaga tcgagtggat cataattttc caattatcaa
    11821 aatcggtgtg ggtttacatt caggaaaact gatgctagga acgataggcg gcgatgatag
    11881 aatggaagga acagtcattt cggatgcagt caatttagca gcacgtttag aaggtcttac
    11941 aaaagtgtat ggctctctaa ttttaacaag tgaagaaacc tttctaaagg caaatggaaa
    12001 agaaaatttt aaatatcgta ggcttggtaa ggcaagggtc aaaggaaaac aggatagtgt
    12061 tgtcatcatc gatatactag atggacaatc tgaattttgg ttagatgtat acctacaaac
    12121 aaaagaagat ttcgaaaaag cattagattt atatgaatca aaggatttta aaaaagcagg
    12181 agagctcttt gataaagttc tagatgtaaa cccttccgat gtggctgctc ggtttttata
    12241 tcaaaaatca ggctactacg aaacacatgg aaccccagaa gattgggatg gaaccgaatc
    12301 cgtttaaaaa atcttttttc ttggttgaca gatttaaaaa tctctgacaa ttaaatcgat
    12361 ttttttttaa ggaaggtgat ctttgaatca aaagttttta attggtgtgt ctgtcggaat
    12421 tctattcaca ccattgtatt cagaaatttt atatctacaa aagccaaaag tgcaggaaga
    12481 agtggctttt ttttctatgc agcctgcaaa ggatccaaat aaaacgaatg tcagttcaca
    12541 aacccagaat acgcaacccg ttctgagttc tcaaccaaaa caattttatt cagaatttat
    12601 cccaaaacca aacgaaccag ttccagaaaa agacaaactg gtaatcccac tcacgcctcc
    12661 ttctgatcca ccccgcttca ttccaaaccc acaccctcaa tttggaaaac catttcccca
    12721 taaattcata gcaagggtaa acttctcagc aaatccagaa aatccacaca aaggagtttt
    12781 attgactcca atcgagtcga ataaagtctt agcaactcta gaggggaaag ttgtagcgat
    12841 tgattatatg gatggctaca ataactatat cattttagag cattccaatg gttattttac
    12901 tgtttatgga aacttagaca ctgtatttgt agcggaggga cagaaagtaa agaaaggaga
    12961 tcacttgggc aatctattaa aggaaaaagg attgtatttt caagtgaatc aaggaaaaaa
    13021 gatattagac ccaatcagtt tcattaaaag ttagataaaa agattggttt tttgacgatt
    13081 gttattataa tagtgaaaat aaaagggata cactcttggc aggaccaata attagaaatt
    13141 acaaagctgg atctattgtt tacttcgaaa aagatagagc agaagatatt tatgtattac
    13201 agagtggacg agtcattttg acctatacat ctgtagatag taagatggaa atcaaagaag
    13261 atgtaaaact cggcgagttc tttggtgtca aatctgcatt aggtagatat cctagggaag
    13321 agactgcaca agtaattgga ggagcaagca ttctagtttt taagcttgct gacttcgaag
    13381 tttttgtggc aaataaaacc cacttgatcc taaagatgat gaaagtcttc tcgagtcagc
    13441 ttagacaaat ccacggtaag gttcgtgaga atcttggtca gtttggagat caaaaatccc
    13501 ctgcttttga attgatgaat gtggcagaag ttttccataa aactggaaat tatgagcacg
    13561 cagtttacgc atacaataag tatttagaac actatccaag tggaccttat gtaaagagag
    13621 cgcaggacct cgtaaaaatt gcggctaaag gaggaatgtt tcctccaaat gtccctgaac
    13681 ttgtttatga accagaaaga agaggagatt ttaatcgacc tgcaaccact tcgaatccaa
    13741 atcctgcggc tacagtaacc cgtattcagg cgttaagcac tgatctatct atttcagatt
    13801 caatcgatga tgcagatgaa aaatatgaat ctggaaactt tcaaggagca ttggatacct
    13861 ataaggcaat cattacgatg ccgcctgccg ctccaggcga agacaaatta atagaaaaag
    13921 cacacttcta tttcggtatg tcgcaaatga aattacaact ttgggattta gcatatgctt
    13981 ctttatccaa ctatgtaaag aaatatccta aaggagaaaa agtcaaagaa gctatttata
    14041 atttaggtgc aatctctgaa agcaaaggag agaaagaaaa ggcagttatg ctctacaaaa
    14101 aagtcgctag tcttccgcca gccgatgaat atacgtcaaa agcaaattca gaaatagcaa
    14161 ggcttggagg ttgatatgct agaagcaatg tttggaaaat ttggaaaagt atttaaacca
    14221 aatgaaatta ttttttgcga atatgaacct ggtaacgact tttatttaat acaagaaggt
    14281 caggtcaaaa tcactaagac agttggctcc tctattaaga cgttagacat cttagaagcg
    14341 ggagacatct ttggagaaat ggcaatctta gaagagcaac ctcgttcagc tactgcggtt
    14401 tgtattactg aagtgagagc tttaaacttc aaccgggcga actttgaact cttaatgaca
    14461 aagaatccac aactagcact tcgagttctc acaatcttct ccactcgtat ctatgatgca
    14521 aagagacgtc tcatgatcct actcatggat gatatcatcg gtaaagtagc ggatgtattt
    14581 ttaatgctct atgaaaaaca aggagcaaat gaacatcaaa aagaagtaac tctcaaagta
    14641 acagtggacg atgtaggaca ctggtgcggc caacctgttg gagaagttca aaaagttcta
    14701 gctcaattta aaaaatcagg caagatcgat atctataacg atcgagtcgt tgtgcacaac
    14761 atccaagagt tccaaaggat catcgcacag aaaagaaaag aaaaataatt cgtctttatt
    14821 gattgctccc gtagctcagg tggatagagc atttgtttcc taaacagagg gccgctggtt
    14881 cgaatccagt cgggggcaat cttcatattt tatttccgtt acgatagttt tttaaattgc
    14941 tccgcaattt gccagttgct ttctcgcttc cagcgcgcac tggcaacctc gctcctattg
    15001 tcgctgcggt tggttcgaat ccagtcgggg gcaatcttca tattttattt ccgttacgat
    15061 agttttttaa attgctccgc aatttgccag ttgctttctc gcttccagcg cgcactggca
    15121 acctcgctcc tattgtcgct gcggttggtt taagtttgtg ctctttatga tttttctaaa
    15181 aacaatagag agtgctaaaa atagaaagtc ttttgctttt ttatattttc tagaattatt
    15241 taaaattcag acattttcaa aaactaaaac tgaatacgat agacgctaat tgggtgttct
    15301 tttcctttta attgaataga gcttttttct tgaaaggtat acccattttc agtctgttgc
    15361 gaaatgttaa agaattaaaa gtcagccttt aaaaataaa
//
LOCUS       DAOQOS010000125        15382 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_1949_length_15382_cov_5.609513, whole genome shotgun sequence.
ACCESSION   DAOQOS010000125 DAOQOS010000000
VERSION     DAOQOS010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 15382)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15382)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15382
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1949_length_15382_cov_5.609513"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(36..605)
                     /locus_tag="PLF66_20770"
     CDS             complement(36..605)
                     /locus_tag="PLF66_20770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66536.1"
                     /translation="MNQLITNYQFNVSAKTITLTDLAEVKLERIRYIKNLSTNTFIYL
                     LTNNNTITTIAGNVITFTGSTTGMNDTDKLAIQYDDGIQVSSESSNSSISTAIGSRSD
                     ASATSDSGTFSIVALLKRLLGKLPTLVSGRLPVDVQSLTVSVDNASIEISNDVGNPIP
                     MSAASLPLPTGAATSVNQNTANTSELLHI"
     gene            complement(606..1292)
                     /locus_tag="PLF66_20775"
     CDS             complement(606..1292)
                     /locus_tag="PLF66_20775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66537.1"
                     /translation="MNHNTLLEKLPSSIYTKESSSNNAKLWKLYAEQANSIEDALLPI
                     YDLNNLSGINLDKFALILGIEREGRSDNDYRNALLNFQNTEIDTASLPALYTAIAKVC
                     PLFTITELCYPREYETRILDGNGFMDGLQNLDPSILRPKIIFRLDGSGVLDGSDCMEP
                     CFIRPASILITILRGQTDNSFLDYSIFEEDIDTVSLFTVSYTASNVAEILSVARSILL
                     PGIAIYIKEG"
     gene            complement(1402..2607)
                     /locus_tag="PLF66_20780"
     CDS             complement(1402..2607)
                     /locus_tag="PLF66_20780"
                     /inference="COORDINATES: protein motif:HMM:NF016736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HMZ66538.1"
                     /translation="MAGITPQGFSGKSREDIILEIETLAKSPELFSHDVDLTPYSPLG
                     IFIALVAESIALQWEAFEAIYYAGFLDTAEGVDLDRIARFAGVTRKPPQKEQIELSFT
                     GTDGTIIPNGFIVQTTSGIQFQTIESKIISGITSVTAEALEYGVSSRVGIGQLTEFAT
                     PQAGIDSVGNLFASTGGASLESDADFRARAIDNINVFRKNVGLIDYIKRQIKNDPNVI
                     SCEIVENQSNIEVNSMPPHSMQFVIDGGSSPYIASLIRKFKPAGIQLVGSILELVSNN
                     NGGYDSIYFSRPGDYDIVVKLSILANAQWQNSNLRKIKTEIIQYVGGIDSYTENGNDF
                     VNEYKGLGVGKKVSVFNIYPLVADFVGIDNLNISLGTTLYNAVNNVITPPSGMRAKLI
                     TANVVVEVT"
     gene            complement(2608..3195)
                     /locus_tag="PLF66_20785"
     CDS             complement(2608..3195)
                     /locus_tag="PLF66_20785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66539.1"
                     /translation="MKYILYDTNGFEELGSELEALGKMEMFNRENPNQKKSFIACESF
                     PPTGKVFEKGSLRDKTEKEKVQSGELNLDKIKDSICEQINKTCYAAIISGFESSALGD
                     IYIYDSEETDQTNLIGAVAVGGSVQYKCTRKSDGKKDFVLHTNTQIKKVLGDAATRKI
                     ALLQKCYQLKQEVLLLNDYDLVVNYPYSAKYSEVI"
     gene            complement(3220..3573)
                     /locus_tag="PLF66_20790"
     CDS             complement(3220..3573)
                     /locus_tag="PLF66_20790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66540.1"
                     /translation="MLTLAIVDKDIQLSKITNDLALKQRIKNRLSLFLGEWFLDTTAG
                     IDWFSVLEKTNASTIQNLVRKEFLREENIVSINSIDVIIIDSLEKAQTYNKPLRTAII
                     NYEVNSIYGVLRGAV"
     gene            complement(3730..3951)
                     /locus_tag="PLF66_20795"
     CDS             complement(3730..3951)
                     /locus_tag="PLF66_20795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66541.1"
                     /translation="MALNKSKLIADLTVFFSDLDPAATAATKAVKLATILDDYVKTGK
                     IKVGTLQSSGTGNLGLPVSSQNISGGEIE"
     gene            complement(3951..4556)
                     /locus_tag="PLF66_20800"
     CDS             complement(3951..4556)
                     /locus_tag="PLF66_20800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66542.1"
                     /translation="MIDADLLNDKFDFELSKIFIGFLGLIESFDKDKMEAKVKPLVKV
                     KNKDEFIELPVIFCPVNTWYSNKTMIIPDYQKGDIVELKGNIYPVQNQLKGISENASP
                     LRFNLSNCTVIGGHEKRPIKISPSLKKEGLVICHENGDFFNLKKDEVKIKSKKIILDG
                     DVAVTGDVEVDKDITWMKKSKPVKASKHIHPTPTGPSGVGL"
     gene            complement(4553..5422)
                     /locus_tag="PLF66_20805"
     CDS             complement(4553..5422)
                     /locus_tag="PLF66_20805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66543.1"
                     /translation="MSTDDVILYARHIEVKIEPVEGEAKVYSYVHGSYLDCFSIEFDV
                     AMGLKAAAFTVKLYNVLKTTVDLCKPQIKGKSKQFPKIYLSAGYGNDIALLASGDIAS
                     YEYKVSDTDSILEIKSINSISKTFNTVITKTFTNQTANQIIESIFKELGINSYSINMK
                     DNSVISQISFSDNLSRILDLLCKKTNTVYYSVFGKFYIESLDEKKGRASQMVLLDRTS
                     GLIGRPEFKGVNLNAKSLLNPKIQQGEVLRVAFRNPETGEDSAFDYKVVKGKHVGGSR
                     IQNYYTEFEARKV"
     gene            complement(5425..5742)
                     /locus_tag="PLF66_20810"
     CDS             complement(5425..5742)
                     /locus_tag="PLF66_20810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66544.1"
                     /translation="MKKILLILVLVVTVGISSNPISNDCKFNGKKLSGKVRIVQAFGD
                     VKVRAVQAFEDIKVLNLSYASSLASCGEWTFVQAGEDFTIQFVEAFEDLKIRFVQAFP
                     GIN"
     gene            complement(5739..6059)
                     /locus_tag="PLF66_20815"
     CDS             complement(5739..6059)
                     /locus_tag="PLF66_20815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66545.1"
                     /translation="MLRIIEINPSEIPIQRTYTIGNVDYTFAFTYNARFDFISVSISL
                     DGEIVFSTKLTYGINMLFGNHTIPFAVVPMAEVDLYKEGFESLKVNKETLGSKVFLYF
                     EDGQ"
     gene            complement(6090..6779)
                     /locus_tag="PLF66_20820"
     CDS             complement(6090..6779)
                     /locus_tag="PLF66_20820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66546.1"
                     /translation="MSSIIGKAKSVFAGRETVGLTGIYQGSETTIDIDLVSSLSTTHN
                     VTVSSHPIEKDKDNTTLNSIVDHVVTANPNVKITCIISSNLGSKTAKDKLKILLAWQS
                     LGALVKLEGYTTGGGILTKVIQTLNRGWGSAFSSELEEPHYVGIDTDLIQNLVLGNIS
                     ITRKSELGEDIEVTIDLTRIQFATAQSVNRTVQKSSAQVKVGSKGQTPVAQTNPAKTV
                     VKGLGKTNFGK"
     gene            complement(6779..8992)
                     /locus_tag="PLF66_20825"
     CDS             complement(6779..8992)
                     /locus_tag="PLF66_20825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66547.1"
                     /translation="MSVLRELKIRLNFEGNVASELKKTNSEVDALKAKFQPLVDNLPP
                     KINQSASAMAELSKATGHSQATLQKMIDATKKDFQLEEQFKKAALAVGLTENEVGRLN
                     SKLKSAEASTANFVNVFKGLAALGITAAAYNFADKAIDESNRAENALIGFKTVVKNTL
                     GVGAVDETTKGVELLFKKLGGSMDMSTIQMSVKNLLGSGFSASQALELVEKNAYMASV
                     NRQAQFKTIGEAVQTYTEGIKNNNAMLTDSTGISENLSVTLKKKGLTMDDLNNTTKRA
                     AVLETIHAMHTKEAGQYLEAYNAQMAGYQGQVIKANAAMSKILQTSGDFIKSALTPLL
                     ALASPVLNFFSDSERGASRTNFALALLGATIGVGLVSATWSWVTSLNAVAVAKALAFG
                     EIIATALAIGAALTAVYVVLEDIFIFFEHGPSASETYFADLLRWLGLSESDLQALSDG
                     FKQFKVFMGEAWEKMKEFANSDAGQFIWKLVKGFALLAASVILLPAIIVGGIAVAFAF
                     IASKWDVIVGYVKKGAILAGKVLLTYLFPLAGLYLFRNEIAMVFDWIVNLIRGTDIGK
                     VFIDKFIDMKNSLGSAVAGIMESAKNVLLDMIPTGALNAMIDSINWVLEKLNQFTSSS
                     VASAVGIEPLNLNMIAHIQARAMGGPIEPNTPYIVGEKGPELRTFDRPGSIIPNDKLN
                     QYQSASSGGGSVNISINANIIASDAKDVSVNLFDKLQDVIDANRNLIKLKLGMAV"
     gene            complement(9198..9671)
                     /locus_tag="PLF66_20830"
     CDS             complement(9198..9671)
                     /locus_tag="PLF66_20830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66548.1"
                     /translation="MAKKIDVVVVGANVNESLGRSVVYVQFEKEGQPMKNQLWALTHP
                     GNLAVERWRDSMLRHDGGKLEFDSSAQTKSFFKECVRPVGEGITEFEEELAAKYGVNK
                     SPKPDPDKLDPRLFGVWQKLQTNFLSGDILDEISEFGESSEPVANPVASTKGNKK"
     gene            complement(9733..10173)
                     /locus_tag="PLF66_20835"
     CDS             complement(9733..10173)
                     /locus_tag="PLF66_20835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66549.1"
                     /translation="MDSGYKKRLFDFDKISVQLLTPFPLDITEGLVLKDSFLSISPVA
                     NNQVNYKVGLGGEVLVSENFNQVHILQIEFIPTAPHIVEIDLLKKAKTQFGILIQNNS
                     APKYKGTASECRILEKPKIDIGTSGFGDFTYKILMTDYVDVFIP"
     gene            complement(10177..10611)
                     /locus_tag="PLF66_20840"
     CDS             complement(10177..10611)
                     /locus_tag="PLF66_20840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004471142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66550.1"
                     /translation="MPNKVFDLDSLSVTIIQNGVPIDISAGLTVDGDFIKVQKATKDE
                     VKIRKGTKGETYSANSIVDNNRTIELTYIPTSSAVSILQSLRKTKEQFGILINSSSQP
                     AWKLSASQCVISEEPDTVINGKDGFKDYTFKIMATDSEQIWL"
     gene            complement(10613..11896)
                     /locus_tag="PLF66_20845"
     CDS             complement(10613..11896)
                     /locus_tag="PLF66_20845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3383 family protein"
                     /protein_id="HMZ66551.1"
                     /translation="MSQINNIVINISLQTLPLAQKGFGMPLIVGTKQPSNLDTVGAIN
                     NVAGYPLGVTTIVVDGFADSGNPIAANDEFVIAGETGSPVHTVVSTSKTSNNTTSITF
                     TPALASSVADNAVVTVTKEKQDVYVEVADQDELLTLGYSSTDDEYKMAKALFSQSPRV
                     EKVAVCYISSFANLETELAELRNEGKDAWYYLLITSRAKADIAIADSYINALEKIGVF
                     ASNDQTITSTGDRTVIIISNNAGDFPDAAIVGRCAAEPIGSITWDSKQMSGQNNSQVT
                     MSEQSTLLAKNFNLIREMGGVDVFWEGKTASGQYIDVIQGRDYLKARLAEALYSLKIN
                     SKKIPAEQRGVDMVEASIREVFREAGRQGVVAGISNATEAAKSDLGDFQYKLTMPVWA
                     ELPFNDRANRKIPISFTATVGGGINTIQVNGVMGV"
     gene            complement(11973..12479)
                     /locus_tag="PLF66_20850"
     CDS             complement(11973..12479)
                     /locus_tag="PLF66_20850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66552.1"
                     /translation="MNTDVVKSLVSALATALDVEIIRAEQGGSMPASDYGSYKVTLKN
                     NEKYQDRRELYIPDPAKYKEEYSRKENFSISLSFISRTGVANIWTLADRAFDFLNVLS
                     VDLKKSLNIYVEMLAGVTDRTTYIDPTYEYKVGFDFLVHGIGKLERVIDAVDIEGTID
                     GIVEVTSP"
     gene            complement(12561..13148)
                     /locus_tag="PLF66_20855"
     CDS             complement(12561..13148)
                     /locus_tag="PLF66_20855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66553.1"
                     /translation="MKVFLFNKNEFSENAIEDETIVRSKVELLNRSLEKEDRYTVIVA
                     EQFPPVGKVFEKGLGLRDKNEKEQVASGEIAMDAIKQRVIDKINELCQKNIISGFESN
                     ALGDVYIYDSEDVDQLNLIGAVASGGSVAYKCTCKADGVRKFYTHTNTQIKKVLGDAA
                     ARKIALLQIAYTHKTAIASLNDYDSIVGYDINKGW"
     gene            complement(13199..13735)
                     /locus_tag="PLF66_20860"
     CDS             complement(13199..13735)
                     /locus_tag="PLF66_20860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66554.1"
                     /translation="MSAKIVDTNNMGEIISGLEELSSRSVDVGILGNADSKLLIYANA
                     NEFGAVIKSRKAMRKLFAMMRELGIKPDKSKRKNQNGIVIPERSFFRSAVDNPANVDK
                     MVKLAEFALNRFFSGQGKAIDVLNAAASSMVASIKNNIASGIDPANSDFTFKKKGHAK
                     TLLGKNPHLIKAVTYEVK"
     gene            complement(13813..14166)
                     /locus_tag="PLF66_20865"
     CDS             complement(13813..14166)
                     /locus_tag="PLF66_20865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66555.1"
                     /translation="MSFKIGDMSKAVSILMGDYIVKRKTQVYEQGELVESFSETPIHC
                     AVLPMTGRELRNLPEGEYDMEDKMILSDGSLDLALGDVIVAFGSEFEIRIKKDVSDLV
                     NLKTYTAKKIPPSPL"
     gene            complement(14209..14616)
                     /locus_tag="PLF66_20870"
     CDS             complement(14209..14616)
                     /locus_tag="PLF66_20870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66556.1"
                     /translation="MRSLRYTQMQISLEQFKQYIPSVTMDDTRIQLYLDDAKRNVERD
                     GFDESHNEFDALQRIMALALMQDDKVAGIKSATTVGNNPEGINSIGVAGINIGFQNPS
                     QVNTVLTSQGKAGYYVDYDILRKKLNGFRGRIA"
     gene            complement(14639..>15382)
                     /locus_tag="PLF66_20875"
     CDS             complement(14639..>15382)
                     /locus_tag="PLF66_20875"
                     /inference="COORDINATES: protein motif:HMM:NF021467.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0042742 - defense response to bacterium
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="encapsulin"
                     /protein_id="HMZ66557.1"
                     /translation="NMRSNLPKIVSTKYVVVQRVYDIVTGIRFTKKERAAFQAKSALG
                     KGPAVQIDTLRVSIARQYVAEKENKIVFLGDSKYNIPGLLNKSGINSVSVADGAAGTG
                     AAKKLWINKTPKEILEDLLIAKDVIESGNVFSAKALVLTPSAMARLRRPYSDANPVPL
                     ITWLKENITFDKILVSNQMATANNGLGVDCFLMMDDSPEVAQIVITSDLSLGEPIYDI
                     LGTSEQVVEESIAGTIIRHPSAIYVGKGI"
BASE COUNT         4748 a         3382 c         2588 g         4664 t
ORIGIN      
        1 caatatgaga agcaatttgc ctaaaatcgt gtccgctaaa tatgtagtag ttcacttgta
       61 ttagcagtat tctgatttac gcttgtagcc gcacccgtag gtaaaggcaa actagccgca
      121 ctcataggaa taggattacc aacatcgttt gaaatttcaa tcgaagcatt atccacactc
      181 acagtaagcg attgcacatc cacaggcaag cgtccactga caagcgttgg aagctttcca
      241 agaagtcttt tcagaagggc aacaatacta aaagtccctg agtcagaagt tgcggatgca
      301 tccgatctag aaccaatcgc agtagaaata ctagaattag aagactcact cgaaacttga
      361 attccatcat catattgaat agcaagttta tctgtatcat tcattcctgt agttgatcca
      421 gtgaatgtaa tcacatttcc agcgatagtt gtgattgtat tattattcgt cagcaaatag
      481 ataaatgtat ttgtgctaag atttttaata tagcgaatgc gttctaattt aacctctgct
      541 aaatcagtaa gagtgatcgt tttagcactc acattgaatt gataattagt aattagttga
      601 ttcatttaac cctctttgat atagattgct atacctggta ataaaatact tcttgccaca
      661 gataaaattt cagccacatt cgaagcagtg tagctaacgg taaatagact cacggtatct
      721 atatcctctt caaatataga atagtccaaa aaagaattgt ctgtttgtcc tcttaagatc
      781 gtaatgagaa ttgaagcagg tcttataaag caaggttcca tacaatcaga accgtcaagc
      841 acacccgagc catctaacct aaaaataatc ttaggacgca agatgcttgg gtctagattc
      901 tgtaaaccat ccatgaatcc atttccatct aaaatccgag tttcatattc tctcggataa
      961 caaagctcgg taatcgtaaa caaaggacaa acctttgcaa tagcagtata cagagcaggt
     1021 aaactagctg tatcaatttc tgtattttga aaattcaaaa gagcatttcg atagtcgtta
     1081 tccgatctac cttctctctc tattcccaaa atcaaagcaa acttatctag attgattcca
     1141 ctgagattat tcaggtcata aataggaagt agcgcatctt caatcgaatt cgcttgctct
     1201 gcatataact tccaaagctt cgcattatta gagctagatt cttttgtgta aatcgaagat
     1261 ggaagttttt ctagtaatgt attgtggttc atacacccac cgacgtaggg aacgcatata
     1321 tgcgttcctt acaattagga cacagtctat cttttactag cccccttttg taaaggggga
     1381 tcaaggggga ttgcataacg gtcatgttac ctccacaaca acattcgcag tgataagctt
     1441 tgcacgcata ccagatggcg gtgtaatgac attattcaca gcattgtaaa gagtagttcc
     1501 aagactgata ttaagattat caattccaac aaagtcagca actaacggat agatattgaa
     1561 gacagaaact ttcttaccaa ctcctaagcc tttgtattca tttacgaaat catttccatt
     1621 ttctgtataa gaatcaatac caccaacgta ttgaatgatt tcagttttaa tcttacgaag
     1681 attcgagttt tgccattgag catttgcaag aatggaaagc ttcacaacga tatcataatc
     1741 ccctggacgt gagaaataaa tcgaatcata accaccattg ttattcgata caagttctaa
     1801 aatagaccca accaactgaa ttccagcagg tttaaattta cgaatgagag aagcaatgta
     1861 aggactagaa ccaccatcaa tcacaaactg catagaatgt ggaggcatag aattgacttc
     1921 aatgttcgat tgattctcta cgatctcaca agaaataacg ttcggatcat tcttgatttg
     1981 acgcttgatg taatcaataa gaccaacgtt cttacgaaag acattgatgt tatcaatggc
     2041 acgcgctcta aagtcagcat ccgattcaag agaagcccca ccagtcgaag caaacagatt
     2101 tccaacagaa tcaatgccag cctgtggagt agcaaactct gttaattgac caataccaac
     2161 acgactagaa acgccatact ccaatgcctc agcagtcaca gaagtaattc cacttataat
     2221 ctttgattca atagtttgaa actgaatccc gctcgttgtc tggacaataa acccattcgg
     2281 tataatcgtt ccatcagtgc cagtaaaact caattcaatc tgttctttct gtggaggctt
     2341 tcgagtaacg ccagcaaacc gagcaatccg atctagatca acgccttcag cagtatctaa
     2401 aaacccagca taataaatcg cctcaaatgc ttcccattgc aaagcgatag actcagcaac
     2461 taaggcaata aaaattccaa ggggagaata aggagtaaga tctacatcat gactaaacaa
     2521 ctcaggcgac ttagcaagag tctctatctc aaggataata tcctctctcg acttacctga
     2581 aaatccttgt ggagtaatcc cagccaatta aatcacctca gaatatttag ctgaataagg
     2641 ataattaact accaaatcat aatcattcag tagtaacact tcttgtttta gttgatagca
     2701 tttttgtaag agtgcgattt ttcgagttgc agcatcacca aggactttct ttatttgagt
     2761 attcgtatga aggacaaaat cttttttacc atctgattta cgtgtgcatt tatactgaac
     2821 agatcctcca acagcaactg caccgattaa attagtttgg tctgtctctt ctgaatcata
     2881 aatataaata tcacccaatg cactggattc aaaaccagag ataatagcag cataacaagt
     2941 cttattgatt tgctcacaaa tggaatcttt tattttatct aaattaagtt ctccactttg
     3001 cactttttct ttttctgttt tatctcgtaa gcttcctttt tcaaaaactt ttccagtagg
     3061 tgggaaactt tcacatgcaa taaaggattt tttttgattc ggattttcac gattgaacat
     3121 ttccatttta ccaagggctt ccaattcgct tcccaattcc tcaaatccat ttgtgtcata
     3181 taaaatatat ttcatttact ctccttgaaa gcgtggttac taaaccgcgc ctctcaatac
     3241 tccatagata gaattgactt cataattaat aattgcagtt ctgagcggct tattgtaagt
     3301 ttgcgctttc tctaaagaat caataataat tacatcaatc gaattaatag acactatgtt
     3361 ttcctctctc aaaaactctt ttcttactag attctgaatt gtgcttgcat ttgttttctc
     3421 taaaacacta aaccaatcaa ttccagcagt tgtatctaaa aaccattctc ctaaaaataa
     3481 agagagtctg tttttgattc tttgttttag tgcaaggtca tttgtaattt tagataattg
     3541 gatgtctttg tcaactatcg ctagagtcag cataattagt agatagtgat tagttattta
     3601 tgattagtca aacgaaaacg ttgagatact aggctgatct tctaatttag tcacgacatt
     3661 taattttccc ctctttgcaa agaggggtgt ctttgttgaa gtaatgcaat atcgttagat
     3721 tggggagatt tattctattt cgccaccaga tatattttgt gaactaacag gcaaccccaa
     3781 attacccgtt ccactgcttt gtaaagttcc aactttaatc ttaccagtct tcacgtaatc
     3841 atctaggatt gtagcaagct taactgcttt agtagccgca gtagccgcag gatctagatc
     3901 agaaaaaaag accgttaggt cagcaattag tttagattta tttaatgcca ttataaccca
     3961 accccactcg gacccgtagg cgtaggatga atatgtttac tagccttcac aggtttagat
     4021 tttttcatcc aagtaatatc cttatccact tcgacgtcac ccgttaccgc aacatcacca
     4081 tctaggataa tctttttaga tttaatctta acttcatctt ttttgagatt gaaaaagtca
     4141 ccgttctcat gacagataac aagcccttct tttttaagag aaggcgaaat cttaatagga
     4201 cgtttctcat gacctccgat gacagtgcaa ttcgaaagat taaaacgaag aggagaagca
     4261 ttttccgaaa tgcctttcaa ttgattttgg acaggatata tattgccttt gagttctaca
     4321 atatcccctt tttgataatc aggaataatc atagtcttgt tcgaatacca agtattaacc
     4381 ggacaaaaaa taacagggag ttctataaac tcatccttgt ttttaacttt gacaagtggt
     4441 ttgactttag cttccatctt atccttatca aagctttcaa taagaccaag gaagccaata
     4501 aagattttac tgagttcaaa atcaaattta tcatttagga gatctgcatc aatcatactt
     4561 tcctcgcttc aaactcagta tagtaatttt gaattctact cccgccaaca tgcttaccct
     4621 taaccacttt atagtcaaac gcagaatctt cacccgtttc tggattccga aaagctactc
     4681 gtaacacttc accttgttga atcttaggat tgagaagact cttagcattc agatttacac
     4741 ctttgaattc aggtctcccg atgagtccag atgtgcgatc cagtaaaacc atttgactag
     4801 cacgtccttt tttttcgtca agagactcga tatagaattt accaaagaca gaataataaa
     4861 ccgtattcgt ttttttgcag agcaagtcca aaattctaga gagattatca ctgaaagaaa
     4921 tttgtgatat aacagaatta tctttcatgt tgatagaata agaattgata ccaagctctt
     4981 tgaaaataga ttcaatgatt tgattagcgg tttgattcgt aaacgttttc gtaataacag
     5041 tgttgaaagt tttagaaata gaattgatag atttgatttc taaaatagaa tcagtatcgc
     5101 taactttata ttcataactt gcaatgtccc cagaggctaa caaagctatg tcatttccat
     5161 aaccagcaga aagataaatc tttggaaatt gttttgactt gcctttgatt tgaggcttac
     5221 aaagatcaac tgtagttttt aaaacgttat aaagtttaac agtaaacgct gccgccttaa
     5281 gacccatagc aacgtcaaac tctatagaaa aacaatctaa ataagaacca tgaacatagg
     5341 aatagacttt agcctcacct tctacaggtt caatcttaac ctcaatatgt cttgcataga
     5401 ggataacgtc atcagtgctc atgattaatt aatacccgga aacgcctgca caaatctaat
     5461 ttttaaatct tcaaatgctt ctacaaattg gattgtaaag tcttccccag cttggacaaa
     5521 tgtccattca ccacaggatg caagggagga agcatagctt agatttaaaa ctttaatatc
     5581 ctcaaaagct tgaacggctc taactttcac atcaccgaat gcttgcacaa tccgaacttt
     5641 accactcagc ttttttccat taaacttaca atcgttcgaa atcggattag aggaaattcc
     5701 aactgtaaca accaaaacta aaatcaataa tatctttttc attgtccatc ctcaaaataa
     5761 agaaatactt tgctaccgag cgtttcctta tttactttta gactctcaaa accttctttg
     5821 tataagtcaa cctcagccat tggaaccaca gcaaagggaa tagtatgatt tccaaatagc
     5881 atattaattc cataagttaa ctttgtagaa aaaacaattt ccccatctaa tgaaatagaa
     5941 acagaaataa aatcgaatct agcattgtag gtaaatgcaa aagtgtaatc tacattgcca
     6001 atagtataag tcctttgaat cggaatttca gacggattga tttcaattat tcttaacatt
     6061 aaagtgtttt ccttatacct gactctgtgt tatttaccaa agtttgtttt ccccaaaccc
     6121 tttacaacag tcttagcagg gtttgtctgt gcgacaggcg tttgtccttt gctaccaact
     6181 ttcacttgag cactggactt ttgaacagtt cgattaacgc tttgagccgt agcaaattga
     6241 atacgagtca aatcaattgt tacctcgata tcttccccta attcagattt acgtgtaata
     6301 gaaatgtttc caagaactaa attttgaatt agatccgtat ctattccaac ataatgaggt
     6361 tcttctaatt cagaagaaaa cgccgaaccc caaccccgat tgagagtttg aataaccttg
     6421 gttaatatac cgccacccgt tgtgtatcct tctagtttaa caagtgcacc tagactttgc
     6481 caagcgagaa gaattttcag tttatctttt gcagtcttac tgccaaggtt actgctaatt
     6541 atacaagtga tcttaacatt agggtttgca gtgacaacat gatcaacaat cgaattaaga
     6601 gtagtattgt ctttatcctt ctcgattgga tgagaagaca cagtgacgtt atgagttgtt
     6661 gacagactag aaaccaaatc aatatcaatt gtagtttcac tgccttgata gattcctgta
     6721 agtccaactg tctcacgtcc agcaaagacg cttttagctt ttccaataat gctactcatt
     6781 atactgccat tcctaattta agtttaatta gatttcgatt tgcatctatt acatcttgta
     6841 acttatcaaa tagatttaca gatacgtctt tcgcatcact agcaatgata ttcgcattga
     6901 tagaaatatt cacactacca ccgccactag acgcactctg atactgattc aatttgtcgt
     6961 taggaataat actaccaggt ctgtcaaagg ttcgaagctc cggtcccttc tcgcctacaa
     7021 tataaggagt attcggttct attggaccac ccatagcgcg tgcttggatg tgtgcaatca
     7081 tatttagatt caaaggttct attccgacgg cactagccac agaactagaa gtaaactggt
     7141 ttaatttttc taaaacccaa ttgatagaat caatcatcgc attcaaggca cccgttggaa
     7201 tcatatccaa gagaacattc ttagccgatt ccattatccc tgcaactgcc gatcccaaag
     7261 aatttttcat gtctataaat ttatcaataa acactttccc gatatcggtt ccacgaatta
     7321 gattcactat ccaatcaaac accatagcaa tttcatttct gaaaagataa aggcccgcaa
     7381 gaggaaaaag atacgttagt agaaccttac cagcaaggat agctcctttt tttacatagc
     7441 caacaatgac atcccattta ctcgcaatga aagcgaaagc aaccgcaata ccgccaacga
     7501 tgatagcagg caataaaatg acagaggcgg caagcaaagc aaaacctttc acgagtttcc
     7561 aaataaattg tccagcgtcc gagtttgcaa actctttcat cttttcccaa gcttccccca
     7621 taaagacttt aaattgttta aacccatcgg ataacgcttg caaatcggat tcagaaagac
     7681 caagccaacg aagtagatca gcaaagtaag tttcactcgc actaggacca tgctcaaaga
     7741 aaataaagat atcctccaat acaacataga ccgcagtaag agccgctcct atagcaagag
     7801 cagtcgcaat aatttctcca aacgccaaag ccttcgcaac cgcaaccgca ttcagggaag
     7861 taacccaaga ccatgtagca gaaactaaac ctacaccgat agtagcacca agcaacgcaa
     7921 gagcaaaatt agttctagat gctcctctct ccgaatccga aaagaaattc aaaacaggac
     7981 tcgcaagtgc aaggagagga gttaacgctg atttaatgaa gtcaccactt gtctgtaaaa
     8041 tcttagacat agccgcgtta gctttaatca cttgtccttg atagcctgcc atctgagcgt
     8101 tatacgcttc caaatactgt ccagcttcct tagtatgcat ggcatgaatt gtttcgagga
     8161 cagctgcccg cttggtagta ttattcaaat catccatcgt aagacctttc tttttcaagg
     8221 tcacagaaag attttcagaa ataccagtgg agtctgtgag catcgcatta ttattcttaa
     8281 taccctcggt ataagtctga acggcttccc ctatcgtctt aaattgcgct tgtctgttga
     8341 cgctagccat atatgcattt ttctcaacaa gttcaagagc ttgcgaagca gagaacccag
     8401 agccaagtaa atttttgaca gacatttgga tcgtagacat atccatagag ccgccgagtt
     8461 ttttaaaaag taactcaacg cccttagtcg tttcgtctac cgcacctaca cccaaggtat
     8521 ttttaacgac agtcttgaaa ccaatcagag cattctcagc gcgattggat tcatcaattg
     8581 ctttatccgc aaaattataa gccgccgcag taatgccgag cgcagcaagt cccttaaaaa
     8641 catttacaaa attagcagta gaggcttccg cagatttaag tttagaattg agtctaccga
     8701 cttcgttttc agtaagacca acagcaagag ccgctttttt aaattgctct tcgagttgaa
     8761 aatccttctt agtagcatca atcatctttt gcagagtagc ctgcgaatga cccgttgcct
     8821 tagaaagttc agccatagcg gaagcagatt gatttatttt aggaggtaga ttatcaacta
     8881 acggctgaaa tttagctttg agcgcatcca cttctgagtt tgtcttcttt aactcagaag
     8941 caacattccc ttcaaagttt agtctgattt ttaattctct caatacactc atcttttctt
     9001 ccttgaaagc gcgaaaacgc tttcgcgcct atctcttccg ctcaaccaac tcagctttaa
     9061 taatctgatt ttctaaatac tcttctcgat acttcatcac ctcatcaatc acgatgaggt
     9121 caaaaggatc tgcttgattg atcgtagtct cagataacaa cgctaagccc gtaataaaag
     9181 gtttccaata ccaaaaccta ttttttattt cctttcgtgc ttgcgactgg atttgcgaca
     9241 ggctcggagg attctccaaa ttcggaaatc tcatcgagaa tgtccccgct aagaaagttg
     9301 gtttgcaatt tctgccacac tccaaacagt ctagggtcaa gcttatcagg atctggctta
     9361 ggagatttat tcactccata cttagccgcg agttcttctt caaactcagt aattccttct
     9421 ccaacaggac gaacgcattc tttaaaaaag cttttcgttt gagcgctaga gtcaaattcc
     9481 aatttgcccc catcgtgtcg aagcatggag tcacgccatc tttcaacagc cagattcccc
     9541 ggatgagtca aagcccaaag ctgattcttc atcggctgac cttccttctc gaattgaaca
     9601 tatacaaccg atctacctaa cgactcattc acgttagccc ctacaactac cacatcaatt
     9661 ttttttgcca ttgtttgtct ccttacaata ttgtaaggaa cgcatacatg cgttccatac
     9721 ggtaggtatt ccctacggta taaatacatc tacataatca gtcatgagga tcttatatgt
     9781 aaaatcccca aagcccgatg tcccaatatc aattttaggt ttctctaaga ttctacattc
     9841 acttgccgtt cctttgtatt taggcgcaga gttattttga atcaagatac caaattgagt
     9901 tttcgctttt ttaagaaggt ctatttcgac gatatgcgga gcggtaggaa taaattcaat
     9961 ctgcaagata tgaacctggt taaagttctc acttaccaaa acctcaccgc ccagaccaac
    10021 tttgtaattc acttgattgt tagcgacagg actaatcgag agaaacgaat ctttcaagac
    10081 aagcccttca gtaatatcaa gcggaaaagg cgtaagaagt tgaacactaa tcttatcaaa
    10141 atcaaacagc cttttcttat atccactatc catagactat aaccagattt gctccgaatc
    10201 agtagccatg atcttaaagg tgtaatcctt aaaaccatct ttgccattga tgacagtatc
    10261 aggctcctct gaaatcacac attgactcgc gcttaacttc caagcaggct gactggatga
    10321 gttaatcaaa ataccaaact gctctttcgt tttgcgaaga ctctgtaaga tggaaaccgc
    10381 gctagaagtt ggaatgtagg tcagttcaat tgtgcgattg ttatctacaa tggaattagc
    10441 agaatacgtt tctcccttcg tgcctttgcg aatttttact tcgtccttag ttgccttttg
    10501 gactttgata aaatcaccgt caaccgttag ccctgcgctg atatcgatag gcacaccatt
    10561 ttgaatgatc gttacgctta atgaatctaa atcaaatact ttgtttggca tattatactc
    10621 ccattactcc attgacttga attgtgttga taccaccgcc aacagtcgca gtaaagctaa
    10681 tcggaatttt acgattcgcg cgatcgttga aaggaagctc tgcccataca ggcattgtga
    10741 gtttgtattg aaaatcccct aaatcagatt tagcggcttc tgtagcgtta gagattcccg
    10801 ctacgactcc ttgtctgcca gcctctctaa agacttctct gatagaagct tctaccatat
    10861 ccactccgcg ttgctctgct ggaatttttt tggaattgat cttgagagag tataacgcct
    10921 cagcaagacg agctttgagg taatctctac cttggatcac atcaatgtat tgacctgatg
    10981 cagtcttacc ttcccagaaa acatccacgc cacccatctc acgaatgaga ttgaaattct
    11041 ttgcaagaag agtcgattgc tcagacatag taacctgaga attgttttga ccgctcattt
    11101 gtttcgaatc ccaagtgata gaaccaatag gctcagccgc acatcttcca acgattgccg
    11161 catctggaaa gtcacccgca ttattagaaa tgataatgac tgttctatct ccggtagatg
    11221 tgatcgtttg gtcgttagat gcgaagactc caatcttctc aagcgcattg atatacgaat
    11281 ctgcaattgc aatatccgct ttagctctcg aagtaatcaa gagataatac caagcatcct
    11341 ttccttcatt gcgaagctca gcaagttctg tttccaaatt cgcaaaagaa gaaatataac
    11401 aaacagcaac tttctctact cgtggacttt ggctaaacaa cgcctttgcc attttgtatt
    11461 catcgtctgt agaggaataa ccgagagtga gaagctcatc ttgatctgca acttctacat
    11521 acacatcttg cttttctttc gtaacggtaa caactgcgtt atctgctaca gaacttgcaa
    11581 gagcgggtgt aaaagtaata ctagttgtat tgttcgaagt cttcgaagta gatacaactg
    11641 tgtgaactgg actaccagtt tcccctgcga taacaaattc atcattcgcc gcaatcggat
    11701 ttccactgtc ggcaaatcca tccacaacta ttgttgtgac tcctagagga tatccagcta
    11761 cattgttaat cgccccaacc gtatctaagt tggagggctg tttcgtccct acgataagtg
    11821 gcataccaaa tcccttttga gcaagtggta atgtttgcag tgatatatta atcactatat
    11881 tattaatttg actcatcctt tattctccta taaatttccc tcgcctttaa ggagagggat
    11941 tgttactaca agcctcacaa ctcctaaaaa attcagggag aggttacttc aacaatgcca
    12001 tctatcgttc cttcgatatc aacagcatca atgactcgct ccaatttacc aattccatga
    12061 actaaaaaat caaagcctac cttatactca taagtcggat ctatatacgt tgttctgtca
    12121 gtaacgccag cgagcatttc cacatagata ttgagagact tctttaaatc gacagacaac
    12181 acattcaaaa agtcaaacgc ccgatctgct aacgtccaaa tattcgcaac cccagtgcga
    12241 ctaataaaac tcaaactaat cgaaaagttt tcctttctag aatactcttc tttgtattta
    12301 gctggatctg gaatatacaa ctcccttcta tcctgatact tctcattatt cttaagagta
    12361 actttataag acccgtaatc agaggcaggc atactaccac cttgctccgc ccgaatgatc
    12421 tcaacatcca aggcagtagc cagcgcagag actagacttt taacaacatc tgtgttcatt
    12481 taattaaccg ttgtatatcg tcgtaaggaa cgcatcgtcg tagggaacgc atatatgtgt
    12541 tccttactat cacgacaagc ctaccaaccc ttgttaatat cataacccac aatcgaatca
    12601 taatcattca aagaagcgat agctgtctta tgagtatagg ctatctgcaa gagagcaatc
    12661 ttacgagccg cagcatcgcc taaaactttt ttgatttgtg tatttgtatg agtataaaat
    12721 ttacgaacac catcggcttt acaggtgcac ttataggcaa cgctaccacc ggaggcaacc
    12781 gccccaatca gattgagttg atctacatct tctgaatcat agatataaac atccccaaga
    12841 gcgttagatt caaaaccaga gatgatattt ttttgacaga gttcattgat tttatcaatg
    12901 actctttgct tgattgcatc cattgcaatt tcgccagagg caacttgttc tttctcgttt
    12961 ttatctcgaa gcccaagacc tttctcaaag actttcccaa caggaggaaa ttgttctgct
    13021 acaattacag tgtatcgatc ttctttttct aaagaacgat tgagtagttc gactttcgaa
    13081 cgaacgatag tttcatcttc aattgcattt tcagagaatt catttttatt aaataggaat
    13141 actttcatat tccgatttac ggataggata acgggagttt gtcagatgct ctaggggatt
    13201 acttcacctc ataagtaact gcttttatta aatgtggatt ctttccgagt aacgtcttcg
    13261 catgaccttt tttcttaaat gtaaaatcag agttagcggg atcaatccca cttgcgatat
    13321 tatttttaat agaagctacc atagaactag cagcagcatt gagaacgtca atagctttcc
    13381 cttgaccgga gaaaaaacga tttaaggcaa attcagcaag tttaaccatc ttgtcaacat
    13441 tcgctggatt atccacagca gagcgaaaga aagaacgctc agggattacg attccatttt
    13501 gatttttacg ttttgattta tcgggcttga tacctaactc tctcatcatt gcaaagagtt
    13561 tccgcattgc cttcctggat ttaattaccg caccgaactc atttgcattt gcatagataa
    13621 gaagtttaga atctgcattc ccaagaatac caacgtcaac agatctagac gataactctt
    13681 ctagcccact gataatctca cccatattgt ttgtatcaac aatcttcgcg ctcattcaat
    13741 cccccttaat ccccctttgc aaaagggggc gtccctaaaa gtataacgtt cccgtattac
    13801 tagccccctt tctcaaaggg gggaaggggg gattttctta gctgtataag ttttcaaatt
    13861 cactaagtca ctcacatctt tcttgatgcg aatctcaaac tctgaaccga atgcaacgat
    13921 cacatctcct aacgcaagat caagggagcc atcagagaga atcatcttat cttccatgtc
    13981 atactcgccc tcaggcaaat tgcgaagctc tctaccagtc ataggaagaa ctgcacaatg
    14041 aattggagtt tccgaaaaac tctctaccaa ctccccttgc tcatacactt gcgtctttcg
    14101 tttaacgata taatcaccca tcaaaatcga aaccgctttc gacatatcac ctattttaaa
    14161 actcataata aatccttcct aattgtaagg aacgcatata tgcgttccct acgcgatcct
    14221 tcccctaaac ccattcaatt tctttcgtaa aatatcataa tcaacataat aaccagcttt
    14281 cccttgggat gtaagaactg tattcacctg agaaggattt tggaaaccaa tgttaatacc
    14341 ggcaacacca atagagttaa taccctcggg attattgccg actgtcgttg cagatttgat
    14401 gcctgcaacc ttatcatctt gcataagagc aagagccata atccgttgta aagcatcgaa
    14461 ctcattgtga gattcatcga agccgtcacg ctctacgttt ctctttgcat catctagata
    14521 caattgaatg cgtgtgtcat ccatagtaac cgatggaatg tattgtttaa attgttctaa
    14581 tgaaatttgc atttgcgtat accgtaagga acgcatatat gcgttcctta cattgggatt
    14641 aaatcccttt ccctacataa atcgcgcttg ggtgtctgat gattgtccca gcgatggatt
    14701 cttctacaac ttgctcggaa gttccgagga tgtcatagat aggctcaccg agagatagat
    14761 cgctagtgat aacgatttga gcaacctcag ggctatcatc catcatgaga aagcaatcca
    14821 cacctagacc gttattcgca gtcgccattt ggttcgatac gagaatctta tcaaaggtaa
    14881 tattctcttt taaccaagtg atgagaggaa caggattcgc atccgagtaa ggacgtctca
    14941 aacgagccat agcggaaggt gtaagaacaa gtgccttagc actaaataca tttccactct
    15001 cgattacatc tttagcgatg agaagatctt ctaaaatttc ctttggagtt ttgttgatcc
    15061 aaagtttttt agcagcccca gtcccagccg cgccatctgc aacggaaaca gaattgattc
    15121 cagacttatt aagaagacct ggaatattgt atttgctatc tccaaggaat acaatcttgt
    15181 tttccttttc tgcaacatac tgtctagcga tagacactct aagagtatcg atttgaactg
    15241 ctggaccttt accaagggca gatttagctt ggaatgccgc tctttccttt ttggtaaagc
    15301 gaataccagt cacaatatca tagactctct gaactactac atatttagtg gacacgattt
    15361 taggcaaatt gcttctcata tt
//
LOCUS       DAOQOS010000126        15327 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_1958_length_15327_cov_5.937336, whole genome shotgun sequence.
ACCESSION   DAOQOS010000126 DAOQOS010000000
VERSION     DAOQOS010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 15327)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15327)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15327
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1958_length_15327_cov_5.937336"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(66..896)
                     /locus_tag="PLF66_20880"
     CDS             complement(66..896)
                     /locus_tag="PLF66_20880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016548610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2797 domain-containing protein"
                     /protein_id="HMZ66558.1"
                     /translation="MMKHEGLTPVSYSFVLAEYPAGIKKTQKAEAKEIVSDFTVNSLL
                     GKRLKLSFTGEIRCVDCGRTTTKSFNQGSCYSCFMNLASNDMCILKPETCHHHLGTCR
                     EPEWGLSNCFKKHTLYLANTSGIKVGITKENPVSKRWVDQGAMYAIPVLEFVSRRDSG
                     IVENTLAKYVADKTSWQKLISSDSLEVDLFKKRDELLSKIKLEELVKEYTIIKKAKLT
                     EIKYPILKYPDKKVSYKPDKDKPIDDILIGIKGQYLLFEKGVINLRSYGGYHLEIEVI
                     "
     gene            complement(911..1543)
                     /locus_tag="PLF66_20885"
     CDS             complement(911..1543)
                     /locus_tag="PLF66_20885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66559.1"
                     /translation="MFKKIRFSEVFKNKVVGNPNYPLILISLFLVSNVSVFAETKWVT
                     IGKAQTDKVLKEFNYDKIEKSVKDKYKFGKESVVNYSMCQENFPELPGQFPCNFLTDE
                     YSIKKNAEDVSSENAAGGKIGIRISKEKNILGGKVFLVGEDKASDGTVNEDMLQVYYL
                     KNNYISHYIFKNQWILFQWKGKKGEETLVSILVLQFKNKEIDSLKRIKFQ"
     gene            complement(1555..2796)
                     /locus_tag="PLF66_20890"
     CDS             complement(1555..2796)
                     /locus_tag="PLF66_20890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008590652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HMZ66560.1"
                     /translation="MHRLIISILISFVFFCRPSTHDTLIRLHPSKSEIIPSSVKFFRD
                     VLPSLSGKRVLLVTNPSGIGVNPEKLMQKFSELDIRLQYLIGLEHGFLGLDEDFSSTP
                     VTIDTTFNVPIYHIYKLKPSEIQLLVSGVDAIIFDVQDMGMRCYTYLTVLKRIMDQVS
                     DKRIPFILLDHVSPGMEVATSGDELRNGYENFAADFPLLFFTGLTLGESALYYNSEFL
                     QNRIDLKIIPVENYSRGMRYEKLGIPWSTPSPNLPNLDSARNYLSLVFLEGINVSVGR
                     GTQAPFVYFGAPWFKDQEEIATQLNSESNQEYYFQPVFFKPTFGPHKDKICRGLRMTV
                     VNLNYNPIRLAYNLVKLLKEKYKKDFKWNGSEKFFIDSLWGGDGFRKAIDTGISYEEF
                     HSTFSMKEEEYAKKIQKYYIY"
     gene            complement(2948..3469)
                     /locus_tag="PLF66_20895"
     CDS             complement(2948..3469)
                     /locus_tag="PLF66_20895"
                     /inference="COORDINATES: protein motif:HMM:NF027363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chalcone isomerase family protein"
                     /protein_id="HMZ66561.1"
                     /translation="MKKIISLLVISLSVVGVDLFAQAKDFTTTGSAVRVKKIAFVNVN
                     VYAITHQMKDVPATRSAQEMINAETDKRFILKMKRNVDSAKIVTAINDAYALNGYSNK
                     ANQDTFSSVLTGDLKENDTITISYNAATKSVTCNYSGKTSTVTGSDFMKATWSIWFGK
                     IDQPSLTQALVGN"
     gene            complement(3613..8571)
                     /locus_tag="PLF66_20900"
     CDS             complement(3613..8571)
                     /locus_tag="PLF66_20900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66562.1"
                     /translation="MICLKCNSFKPGFVGRLCPVCSEPLQLETNSNRDKLVLGRIRDY
                     ITKWQKTGKISKKSLTEFELALEAESNPEPKEISVGFIEMVLKYLQGLLFSFFSGFSK
                     IFEPILIKNDETSFTKKSGKRDIDASFESATDALFNQDNSNLTGIEALTELDESKKTT
                     GKYRNANYPKYKSEENFEIWKGLQIFFNEYIWWFIGSLLVLSGSIMGIREAWNVLSGV
                     NRHLTVLLAVVIYQYLFVSLGIFLGKRLNVTGKLLSSISLLLLPISYSVSSDLIIANF
                     EIGLVAVFCLIGITAILLNLVQKQFISEQKKYLYFVIPSLLVQGFIPWLGAGFFTNLF
                     IFLPLILLFQMSRIWAKSESGNNLSFLLSIYGSIGGGVVYFLQLMNATLLSIGSFEFT
                     VLLLWLLIFNIILSTAFGSIAIQFHSKKGFHILEVIFLAIVIVLASTSGLSLNLLTNS
                     DSFTVRILLLIIPYLATHVFFITIKRHTASIHPFMLLSIFSSFLTGLVFFPLTDWSFA
                     FVTFIPVLGIYFTKTSNRDLKISLLAWGYSTGVSILFYLYYKSFFVADNQSYSLLLPL
                     LFVGTFLSVVSHIVGGYSRSIFHIIGGGSLYFTFYGVITILLPFAEMFSRIGISFVLL
                     ASLYSILAILFDEKLRSKEDGSLFPFDDVSLLACLVGLLFSFFSIRSTTFENSILLFS
                     GFISILRSNRDKSSLSSFIGAMIFSLGIFRICIDLFGDPRISSLVLSSSIIASTLGLL
                     CILFPNINPEPIVSRKAFYFIRLPFPAQSPVLIRNALSATSLLYVITSSFLFFNWLSL
                     NELPERNYVITSMGIIVILFLIGFFTRALNSFQLRGSSLALALIFVFIGFAAIANRIG
                     RPLPPKIVGINLTLGIIALWVLVQFFIYKGDRIANWLDNLAQGKYYPYIPLAAMCIIG
                     FVLLVDVFLLNPNSLERFLYITPPTFFIGSGLSAFLFFLSTRLFVSLSISLLLFSIAF
                     GLAFTQESITGIELVRLSQSAVNWVPFSTSEMTRIADWSDPIYFLPRDLSRVILIYRF
                     TLGILVAGLIYSSLSFLLNLKLISDLFKRFQNSLEHSIYIQFTFANWSFLIFCFLCLV
                     SYKYAFDTTGYLSLASGGLLVFSGLSRQGTFMVAMSVLLLIHGYSHNDSIYPSWSGPL
                     LAFIGFLLIILVRPVSSITKKQFSRILESYHAGSFIYSSIGIVYALAAFSPTFRENAV
                     PRLVSGALSGFLGGWTNSYVLGLTLFIIAISLWIGARHWTHGMTSVGVYTSSLFFGFA
                     GITIFPFFTRFLFIGLVNLNLLEMIPFFAFIVTIVSIFYFYSSIVLNQGRADITIGAS
                     YGSDTLILLVGLFLAIFIRFQISSSIPYASLILVITILLIITLTLTESFILRRSHHFY
                     FSQVSIASFYLVLKPSFPELLTPEVDALFALIFGFILTGITAIARSAGIPPLEQSTRR
                     FAAFMPVIAGIVLPTDLNYTNAGFATFTAALYAALASTSSNKIYSVLAALAASLAIFT
                     GIMASNTQGFEIYLAPIGLFSIFLAHIFRENLIDTAKKTIRLFGGLMIYLPAAVNISF
                     ELGQASDPMYAISFGILSLLGVIAGMIFQIRSYLFMGILFFTLDIAVNLLQTGLRDQR
                     MGFMLLSLTGLSIISGLILYSLKKEKIIVFLNRMKKKLSDWE"
     gene            complement(8580..10046)
                     /locus_tag="PLF66_20905"
     CDS             complement(8580..10046)
                     /locus_tag="PLF66_20905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001162801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HMZ66563.1"
                     /translation="MSVEKSGEKPEDIFEHEFEILKQIKEKFEITKDGNSNLVEYKIL
                     GENYEQLLKQSVKLMKIGDSTQRRLIKTQKQLQDANEQIEKSYNNLKALSQFGQSITA
                     SLDTKEIILSLLHHIQPMMPTDILSIGMYEEDKNIIKYKFCVKNGAYVPSLASERLED
                     KNFSSLCYQKKQEIIVHNLEKEHPDLVSTIKELWDEVTQSLSFFPLKVEDRFIGILTV
                     QSYQPEAYSENQLNVLRTLASYIGIAIDNADAYKNLSKKNKQLNDNIEKINSLNEGLE
                     KERQKSEKLLLNILPASIAERLKAGESVISDYFPEATVMFADLAGFTKMSAKLASPEK
                     LVIILNEIFTEFDQVANKYGLEKIKTIGDCYMVAGGIPVSSKDHSERCGYASLEMLKV
                     FTQIRDNWKMDFGIRIGIHVGSIVAGVIGQNKFVYDLWGDTVNTASRMESHGVPGRIN
                     CSQDLFEKLKDKFIFEDRGEMEVKGKGVMHMYFLNDKK"
     gene            complement(10053..10427)
                     /locus_tag="PLF66_20910"
     CDS             complement(10053..10427)
                     /locus_tag="PLF66_20910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004753666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1987 domain-containing protein"
                     /protein_id="HMZ66564.1"
                     /translation="MDKILIQKTKTSPEIIMDFEKGILDIIGESYPENAVGFYKPVFD
                     WLNSVINANKALTVNFRLDYFNTSSSKCVIDILDMLDKYHENSGTVSVKWYYKEDDDD
                     MLETGEEFSSDIKVPFELLSYS"
     gene            complement(10427..10981)
                     /locus_tag="PLF66_20915"
     CDS             complement(10427..10981)
                     /locus_tag="PLF66_20915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008595640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SiaB family protein kinase"
                     /protein_id="HMZ66565.1"
                     /translation="MNQMGGLYSQYLDIKDHKILVSFKGAISQEVLTEFGTMIRSSLS
                     AETNTKKIFAVFVELAQNIVHYSAEYKEHANGRAGIGIIILRERHDLFFLSSGNMVLN
                     DRVEKIKAKCEKVNSMNKDELKAYYQELIRGQRPTADGSKGAGLGIVDIARKCDSPIE
                     YVFDRVDDTMSFMTLSVYFKKDNQ"
     gene            complement(11167..>15327)
                     /locus_tag="PLF66_20920"
     CDS             complement(11167..>15327)
                     /locus_tag="PLF66_20920"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HMZ66566.1"
                     /translation="KILTALGSNGKVITDVIPELEFIIGEQPPVAELGAQENSNRFYL
                     VFQNFIKVFANYEHPLAIFLDDLQWADSASLGLIKNLMLDSSVRYLFLMLAYRDNETD
                     NSHPFVIMLDAIKKEGGVVDSIYLKPLEEQHVNELLCDSLFTKPEDVKLLSELVFQKT
                     GGNPFFITELLKQLTKDDSISFDYENGKWKWDIEKIRSTRISENVVELLIGRIQKLSE
                     KTQHILKLASCIGNNFELATLSLINNAWPQDTKRDLSETIKEELIYPVGDNYRLVDSM
                     GTDIKDMRRNYETAKKIAYRFQHDRVQQASYELIGQEEKKSLRLKIGRILLKNEGWEE
                     NLFDVVNHLNIGSDLITDESEKKNLIELNLQAGKKAKISTAYKPALEYLSQAHRILNQ
                     FSKPWEKYYELSIIVYKELGEVEYLNGNFENSEKLIGIILDHAKTDIEKGEAYNMLMI
                     QYTVEGKFDKALPTVIKALKPLGLSLPSENFQDIIAEESKEVTKNLGNRKVPDLINEA
                     KMQNPTMEMAVKLLMNTITTAYNIAIELFPVICLKMVNIFLKYGNLPESYGYSAYGIM
                     LASSGEYRNAYSYSLLSLQISQKFESKAGIAKAANILANYANPWNHHLILSDKINRDG
                     IQAALDSGEFMHGGYSALHIMVNSFYTGKTFSSMMEDLPKQLDFSVHAKNVMAIDTIL
                     GVEIIVSNASGKTSGKDVFQSNSYNEKSYLDLCNLHQSMYPITIYKIMKCQMLFLYEM
                     HDEMLSLILETEKMIPFINGINSVAEHNFYYSLILAAVYKDASKEDRIKYINQIKTNQ
                     KQMKIWAESCPENYLHKYLLVEAEIARLEYKNWKAAKYYDESILEARKNEFVQNEALA
                     NELAAKFWLKKKNYRFAQNYFLEAYTCYEGWGAIRKCEDLKERYSNLITDQKIPYDFS
                     KTLSGTMSISTQTGSGSATQLHTTNTLDLQSVLKSSSAISGEIKIENLLNKIMSIVVE
                     NAGAQRGVLLLKKDKKLYIEAEGSISSDDVKVLTNIPVDEYPDIASSVVYYVERTKES
                     KVLINATTDDKFNKDTYISRNQIKSILCSPIIKQGELTGVLYLENNLSSGAFTADRLQ
                     VVNVLSSQAAISIDNALLYANMEQKVRDRTKELAETNEQLAEKNQHITDSINYAKTIQ
                     EAILPSKSGIGTVLKDFFILFRPKDIVSGDFYWFTHFEGYTFIAAVDCTGHGVPGAFM
                     SMIGSSILNQVVKEQHILDPALILEHLNNNVRHALRQDVKEDASRDGMEICFCRIDST
                     GKEVVFAGGHRTLYMMKGDEFVSIKGDKESIGGKQKEARKYTNHIIRIESGVRNALYL
                     TTDGFQDQPNPDGKKIGSKGLQEMIQLHYNKSGLDQKKLFEDGLDAHTQNNKEPQRDD
                     ITVIGIIL"
BASE COUNT         5236 a         2780 c         2278 g         5033 t
ORIGIN      
        1 gaccagagat ttacagatat aaatgaaact atgaatattg ataatattcc ccaattcatc
       61 gctaactata taacctcaat ttctaaatga tatccgccat aggatctcaa attgattact
      121 cctttttcaa ataacaaata ctgtccttta attccgatca aaatatcgtc tatcggctta
      181 tctttgtctg gtttatagga aactttctta tcagggtatt ttaaaatggg atattttatt
      241 tcagttaatt ttgctttttt tattattgta tattctttga ctaattcttc taattttatt
      301 tttgaaagta attcatctct ttttttaaat aaatctactt ctaaagaatc actgctaatt
      361 agtttttgcc aagatgtttt atctgcaaca tacttagcca aagtattttc tacaattcct
      421 gaatctcttc tagatacaaa ctctaaaacg ggaattgcat acatggctcc ttggtccacc
      481 catcttttac taacaggatt ttcttttgta attcccactt taattccgct ggtattggca
      541 agataaagcg tatgtttttt aaaacaatta ctaagtcccc attctggctc tctacatgtt
      601 cccaagtgat gatggcaagt ttctggtttt aaaatacaca tatcattact tgccaaattc
      661 ataaagcagc tataacaaga tccttgatta aaacttttag tagtcgttct accacaatct
      721 acacatcgaa tttctccagt aaatgaaagc ttgagtcttt tgcctaaaag agaatttaca
      781 gtaaaatcgc ttactatttc ctttgcttct gctttttgag ttttctttat tccggcagga
      841 tattctgcca aaacaaaaga gtagctaaca ggtgttagcc cctcatgttt catcattcta
      901 agaaagccct tcactgaaat tttatccttt ttaatgaatc gatttcttta tttttaaatt
      961 gtaaaaccaa aatggaaacc aatgtttcct cccctttctt acctttccac tgaaataaaa
     1021 tccactgatt tttgaaaata taatgagaga tataattatt ttttagatag taaacctgta
     1081 gcatatcttc attcacagtt ccatcggatg ccttatcctc tcctactaaa aacacctttc
     1141 cacctaatat gtttttttcc tttgagattc taattcctat cttcccacca gcagcatttt
     1201 cactacttac atcttctgca ttttttttaa tagaatattc atctgttaaa aaattacaag
     1261 gaaactgacc cggaagctcc ggaaaatttt cctgacacat tgagtagttt acaaccgact
     1321 cttttccaaa tttatactta tctttaacag atttttcaat tttatcgtaa ttgaattcct
     1381 tcaagacttt atctgtctgt gctttgccta tcgtaaccca ttttgtttct gcaaaaacgg
     1441 atacattgct gactagaaac aaactaatca gaatcagcgg ataattagga ttacctacta
     1501 ctttattttt aaatacttcc gagaatcgga tttttttaaa cattttatct ccttttagta
     1561 aatataatat ttttgaattt tttttgcata ctcttcttct ttcatagaaa aagtagaatg
     1621 aaactcttca taagaaatac ctgtatcaat cgcctttcta aaaccatctc ctccccaaag
     1681 actatcaata aaaaattttt cagatccatt ccatttaaaa tctttcttat atttttcctt
     1741 caataacttt actaagttgt atgctaatct tatcggatta taattcaaat tcacaacagt
     1801 cattcgaagg cctctgcata ttttatcctt gtgaggacca aaggttggct taaaaaaaac
     1861 aggttgaaag taatattctt gattcgattc tgaatttaat tgagttgcaa tttcctcctg
     1921 atctttaaac caaggagcac cgaaatagac aaagggagct tgtgtgcctc ttcctacgga
     1981 aacattaatt ccttccaaaa acacaagaga taaatagttt ctagcagaat ctaaattagg
     2041 taaattcgga gagggagtgc tccatgggat tcccagtttt tcatatcgca ttccccttga
     2101 ataattctcc acaggtataa tttttaaatc gattctattt tgaagaaatt cagaattata
     2161 ataaagagca gattccccca aagtgagtcc agtaaaaaat aaaagaggga aatcagctgc
     2221 aaaattttca tagccatttc ttaactcatc tccactagta gctacttcca ttccgggact
     2281 tacatgatcc aataatataa atggaattct tttatcggat acttgatcca taattcgttt
     2341 taataccgtt aagtatgtat agcaacgcat tcccatatct tgaacatcaa aaataattgc
     2401 atctacccca ctaacaagca attgaatttc gcttggcttc aatttgtaga tatgataaat
     2461 aggaacatta aaagttgtat caatcgttac tggagtagaa ctaaaatctt catctaatcc
     2521 aagaaaacca tgttcaagac caatcagata ttgcaaacgt atatctaact cagaaaactt
     2581 ttgcattaac ttctcaggat ttacacctat tccagaagga ttggttacga gcaatactcg
     2641 ctttccagat aatgagggta agacatccct aaaaaacttt acactagatg gaataatttc
     2701 ggacttagat ggatgaagtc gaatgagtgt gtcatgagta gagggtctac aaaagaaaac
     2761 aaaactaata agtatagaaa taattaaacg atgcatatat tacctatgaa aattaataat
     2821 cattctaaac aagaatgatt tctttccata caataatatt gaatagatta tgaaagcagt
     2881 tttaaaaaat tatctagata ggatttgtaa attcgacacg gataagaccg tgccgaatca
     2941 gattggatta atttccaact aaagcttgtg ttaaagatgg ttgatcgatt tttccaaacc
     3001 aaatagacca agtcgccttc ataaaatcag aaccagttac agttgatgtt tttccactgt
     3061 aattgcaagt aactgattta gttgctgcgt tatacgaaat ggtaatagta tcgttctctt
     3121 tgagatctcc agttaataca ctgctgaaag tatcttgatt tgccttattg gaatagccat
     3181 ttaaagcata cgcatcattg attgcagtta caatttttgc agaatccaca tttcttttca
     3241 ttttcaaaat aaacctttta tctgtttctg cgtttatcat ttcctgtgca gaacgagtag
     3301 caggtacatc cttcatttga tgtgtaatag catatacatt tacattgaca aaagcaattt
     3361 ttttaactcg aactgctgaa cctgtagttg taaaatcctt tgcctgtgca aataaatcta
     3421 cccctactac cgataacgaa ataaccaaca aagaaataat ctttttcatt ctatatcctc
     3481 taaatgtgtg attgtttatg agtttgcact tcaccaaact cacttatgtt agacaattct
     3541 taagttggat tgggacgtta tataattaaa aaaaaataat ttatgatttt tttttcctgt
     3601 attcaaagta aattattccc aatcggataa tttctttttc attctattta ggaagacaat
     3661 aatcttctcc ttcttgagac tataaagtat taacccagag atgatggata gtccagtcag
     3721 ggaaagtagc ataaaaccca ttctctgatc tctaagccca gtttgaagaa gattgactgc
     3781 aatgtctaat gtgaaaaata agatccccat aaatagataa gatctaatct gaaaaatcat
     3841 tcctgctata accccaagta atgataaaat accaaaggat atggcataca ttggatcact
     3901 cgcttgtcct aattcaaaac ttatattcac tgccgcgggt aagtagatca taagcccgcc
     3961 aaatagtctt attgtttttt tagcagtgtc aattagattc tctctaaata tatgtgctaa
     4021 aaatatagaa aacaatccga taggtgctag atagatttca aatccttggg tattcgatgc
     4081 cataatacct gtaaatattg ccaaacttgc cgcaagtgct gctaaaactg aataaatttt
     4141 attagaggaa gttgaggcaa gagcagcata aagagccgca gtaaaggtag caaaacctgc
     4201 atttgtatag tttagatctg ttggtaatac aattccagca atcacaggca taaatgccgc
     4261 aaatcttcta gtgctttgct ctaaaggtgg aattccagca ctcctcgcta ttgctgtaat
     4321 tccagtcaga ataaatccaa agattaaagc aaagagtgca tctacttcgg gagtaagcaa
     4381 ttcaggaaaa gaaggtttta aaactaaata gaaagatgct atactaactt gggaaaaata
     4441 aaaatgatgc gatctgcgaa gaataaaaga ttctgtaaga gttaaggtta tgatcaatag
     4501 aattgtaatg acaagaatca aggaagcata tgggattgaa gaagaaattt gaaaacgaat
     4561 aaaaatagct aagaataacc caaccaaaag tattaaagtg tccgatccat agctagcacc
     4621 aattgtaata tcagctctac cttgattcaa tactattgag gaataaaaat aaaaaataga
     4681 aacaattgta acaataaaag caaagaaagg gatcatttct aaaaggttaa gatttactaa
     4741 tccaataaaa agaaatcgag taaaaaaggg gaaaatagtg attcctgcaa aaccaaaaaa
     4801 taaactacta gtatagactc ccacagaagt catgccatgt gtccaatggc gtgccccaat
     4861 ccaaagagaa attgctataa taaagagagt aagacctaga acatacgaat tggtccatcc
     4921 tcctaaaaat ccactcaaag ccccagaaac taatctagga accgcgtttt ctctaaaggt
     4981 aggagaaaaa gcagctaacg catatacaat tccaatactc gaataaataa aagatcctgc
     5041 atgatagctt tctagaattc tactaaattg tttttttgta atagaactaa caggtctcac
     5101 aagaatgatt agcaggaatc caataaatgc taaaagagga cctgaccaag aaggatagat
     5161 tgaatcatta tgagaataac cgtgtatcaa aagtagaaca ctcatagcta ccataaaagt
     5221 tccctgtctc gataaaccag agaatactaa caacccaccg gaagctaaag aaagatatcc
     5281 tgttgtatca aatgcatatt tataggaaac taaacaaaga aagcagaaaa tgagaaagga
     5341 ccaatttgca aaagtaaatt gaatgtaaat ggaatgctct aaagaatttt gaaaacgctt
     5401 aaataaatct gaaattaact ttaaattaag taaaaaagat agagaagaat aaatcaagcc
     5461 agcaactaaa atccccaaag taaagcggta aattaatatt acccgcgata gatctctagg
     5521 aagaaaataa ataggatcag accaatctgc tatcctagtc atttcagagg tactaaacgg
     5581 aacccaatta actgctgact gagaaagccg aactaattct atacctgtaa tactctcttg
     5641 cgtaaatgcc agaccaaatg caatgctaaa taataggagt gaaatggaga gagaaacaaa
     5701 caatcgagta gacagaaaaa ataaaaatgc tgaaagacct gagccaataa aaaaagttgg
     5761 tggagttata tataaaaaac gctctaggct attcgggttt aaaagaaaaa cgtctactag
     5821 tagaacaaat cctataatac acatagcagc caaaggaata tatggatagt atttaccctg
     5881 tgcgagatta tctaaccaat ttgcaattct atcaccttta taaataaaaa actgaactaa
     5941 aacccataaa gcaatgatgc ctaacgttaa attaattcct acaatctttg gaggaagagg
     6001 acgcccgatt cgatttgcaa tagctgcaaa accaataaag acaaatatta atgctaaagc
     6061 aagactagat ccacgcaatt gaaaggaatt gagtgctctt gtaaaaaatc caattagaaa
     6121 taaaataaca ataattccca ttgaagtaat tacataattt ctctcaggta attcatttag
     6181 agaaagccaa ttaaaaaaca aaaaagaaga cgttattaca tagagcaagg atgttgcaga
     6241 caatgcattt ctaattaata ctggtgattg cgcgggaaag ggcaatcgaa taaaataaaa
     6301 tgcctttcta gaaacaattg gttctggatt tatattggga aataaaatac ataaaagacc
     6361 taatgtagat gcgattatac tagaagacag aaccaaagaa gaaattctag ggtcaccaaa
     6421 taaatcaata cagattctaa aaatccctag agaaaaaatc atggctccaa taaaacttga
     6481 taaggaactt ttatctcgat tagaacgcaa aatagaaata aatccgctaa aaagtaaaat
     6541 tgaattttca aatgtagttg atcgaatcga aaaaaacgaa aaaagcaaac caactaaaca
     6601 agcaagaaga gatacatcat caaaaggaaa aagggaacca tcctccttag atcgcaactt
     6661 ttcatcgaag agaattgcca gaatactgta aagacttgct aatagtacaa aactaattcc
     6721 aatacgggaa aacatttcag caaatggcaa taaaattgta atcactccgt agaatgtaaa
     6781 ataaagactc cctccaccta tgatatgaaa aatagaccta gaatatccgc cgactatatg
     6841 agataccacg cttaaaaatg tcccgacaaa aagtaatgga agtaagagcg aataagattg
     6901 attatccgct acaaaaaaac ttttgtaata gagataaaaa agaatagaaa cccctgtcga
     6961 gtatccccaa gccagtagag agattttcaa atctctgtta ctagttttag taaaataaat
     7021 tcctaggacg ggtataaacg ttacgaatgc aaaactccaa tctgtgagcg gaaagaatac
     7081 taatcctgtt aaaaatgaac taaaaatgga taaaagcata aagggatgaa tagaagcagt
     7141 atgtcttttt atggtaataa aaaaaacatg agttgctaaa taaggaatga ttaataaaag
     7201 aattctgaca gtaaacgaat ctgaattagt taataaattt aaactcaatc cagaggtaga
     7261 agccaataca atcacaatcg ctagaaaaat aacttctaga atatggaatc ctttttttga
     7321 gtgaaattga atagcaatac taccaaaagc tgttgaaagt ataatattaa aaataagcag
     7381 ccagagtaaa agaactgtaa actcaaatga tccaatggat aaaagagtag cgttcattag
     7441 ttgtaaaaaa taaactactc cgcctcctat cgatccatag attgagagta gaaaggataa
     7501 attatttcct gattcacttt ttgcccaaat tcgactcatt tgaaatagta ggattagagg
     7561 taaaaatata aatagatttg taaaaaatcc agcaccaagc caaggaataa aaccttgcac
     7621 tagtaaagaa gggattacaa aatataaata tttcttttgt tcgctaatga attgtttttg
     7681 aacaagattg agtaaaatcg ccgttatccc aatcaagcag aaaactgcaa ctaaaccaat
     7741 ctcaaaatta gctataatca gatcggatga aacggaataa gaaataggca atagcaataa
     7801 ggatatggaa gataatagtt tcccagttac atttagtctt ttacctaaga agataccaag
     7861 gcttacaaat agatattgat aaatcacaac ggctaataaa accgttagat gccgattcac
     7921 tccagagagc acattccatg cttctctgat ccccatgata gatccagata aaactaaaag
     7981 agaaccaatg aaccaccata tatactcatt gaagaaaatc tgcaatcctt tccaaatttc
     8041 aaagttttct tccgatttat atttcggata attggcattt ctgtatttcc cagtagtctt
     8101 ttttgattca tccaattccg tgagggcttc aattccagtt agatttgagt tatcttgatt
     8161 aaataaagcg tctgtcgcac tctcaaagct agcatctata tctcttttac cggatttttt
     8221 tgtaaaactt gtctcatcat ttttaattaa aataggttca aaaattttac taaatccaga
     8281 aaagaaagaa aatagtaagc cttgtaaata tttcagaacc atttcaataa atccaacaga
     8341 aatctcttta ggctctggat ttgattccgc ttccaatgct aactcaaatt ctgttaatga
     8401 ttttttacta atttttccag ttttctgcca tttggtgatg tagtctcgaa ttcttccaag
     8461 aactaatttg tcccgatttg agtttgtttc taattgtaag ggttctgaac agacagggca
     8521 gaggcgacca acgaagcctg gtttgaaaga attacattta aggcaaatca tagtttgccc
     8581 tactttttat catttaggaa atacatgtgc atcacacctt ttccttttac ttccatttct
     8641 ccgcgatctt caaaaataaa tttatctttt agtttttcaa ataaatcttg agaacagtta
     8701 attcttccag gaactccatg agactccatc ctagaggcag tattaactgt atcaccccat
     8761 agatcatata cgaatttgtt ttgtccgatg actccagcta caatggatcc tacatgaatt
     8821 ccaattcgta ttccaaaatc cattttccag ttatctctta tttgagtaaa aactttaagc
     8881 atttctagag atgcatatcc acatcgttca ctatgatctt tacttgaaac aggtatacca
     8941 ccggctacca tataacaatc gccgatcgtt tttattttct ctaaaccgta tttattggca
     9001 acctgatcaa actcggtaaa aatttcattt aatataatta ctaacttttc aggtgatgca
     9061 agcttagcac tcatctttgt aaaaccagcc aaatcagcaa acatcacagt tgcttcagga
     9121 aaataatctg aaataacact ctcgcctgcc tttaggcgtt ccgcaattga tgctggaaga
     9181 atatttagta ataacttttc cgacttttgt ctttctttct ctaaaccttc attgagagag
     9241 ttgattttct caatattatc attcagctgt ttgttttttt tagaaaggtt tttatatgca
     9301 tcagcattat caatggctat acctatatag gaggctaacg ttctaagaac attgagctga
     9361 ttttctgaat aggcttctgg ctgatagctc tggacagtta gaattccaat aaatcgatct
     9421 tctactttga gaggaaaaaa ggagagagat tgagtcactt catcccacag ttcctttata
     9481 gtggaaacta aatctggatg ttctttttct aaattgtgaa caatgatttc ctgttttttt
     9541 tgatagcaaa ggcttgaaaa attcttatcc tctaagcgtt ccgatgcaag ggatggaaca
     9601 taggctccat ttttcacaca aaatttatat ttaattatat ttttatcctc ttcatacata
     9661 ccaatggata ggatatcggt aggcatcata ggttgaatgt gatgtagaag agaaagaata
     9721 atctcttttg tatcaaggct tgcagtaata gattgcccaa attgacttaa tgctttcaga
     9781 ttattgtagg acttttcaat ctgttcgttt gcatcttgga gctgtttttg tgttttaatg
     9841 aggcgtctct gcgtagagtc gccgattttc attagtttga cgctttgctt taggagttgc
     9901 tcataattct caccaagtat tttatactcg actaggttcg aatttccatc tttcgttatt
     9961 tcaaattttt cttttatctg tttgagtatt tcaaactcat gttcaaaaat gtcttcaggt
    10021 ttttctccag atttttcaac cgacattttt ttctaggaat aagataatag ttcaaaggga
    10081 acttttatat cgctagaaaa ttcttctcct gtttctagca tgtcatcatc gtcttcttta
    10141 taataccatt taacagatac tgttccagaa ttttcgtgat atttatctaa catatctagt
    10201 atatcaatta cacacttgga ggaacttgta ttaaaataat ctaatctaaa attgactgtg
    10261 agcgctttat tcgcattaat aactgaattt aaccaatcaa agactggctt ataaaatcct
    10321 acagcatttt caggataaga ctcaccaatt atatctagta tacccttctc aaaatccata
    10381 ataatttccg gagaggtttt agttttttgt ataagaattt tatccattat tgattgtcct
    10441 ttttgaaata aacagataga gtcataaacg acattgtatc atctacgcga tcaaatacat
    10501 attctatggg gctgtcacat ttgcgagcaa tatcaactat gcctaatcca gctcccttac
    10561 tgccgtcagc agttggtctt tgacctcgta taagttcctg gtaataagcc ttaagttcat
    10621 ccttattcat agaatttact ttctcacatt ttgcttttat cttttctact cggtcattta
    10681 ataccatgtt tcccgaactt agaaaaaata aatcatgcct ttctcggaga attataattc
    10741 caatgccagc tcttccattt gcatgttctt tgtattctgc agaatagtgg actatatttt
    10801 gtgcaagctc tacaaataca gcaaagattt tctttgtatt tgtttctgca ctcaaagatg
    10861 atctgatcat agtgccaaac tcagtcaata cttcctgaga tattgcacct ttaaaagaaa
    10921 caagtatctt gtggtctttt atatcaagat actggctata taatccaccc atctgattca
    10981 taagttttta cactaaccta aaatttataa tattgtcaat aatatcttta ttattgattt
    11041 cgaacagaat agaaaccgtt taaacgactc aatccgcaaa aaaatgtccc tagataacct
    11101 aagaacaaat agcagtatca cataaacctt ttttgagtca acccaattaa gcaaaaatct
    11161 attccattat aaaattatac caatcacagt gatatcatct ctttgtggct ctttattatt
    11221 ttgtgtatga gcatctaaac catcttcaaa gagtttcttt tgatctaaac ctgatttatt
    11281 ataatgcaat tgaatcatct cttgtagtcc ctttgatcca atcttcttac catcaggatt
    11341 tggttgatct tgaaatccat ccgttgttaa gtataaagcg tttcgcacac ctgactcaat
    11401 tcgaatgata tgatttgtat atttgcgagc ttctttttgt ttaccaccaa ttgactcttt
    11461 atctccctta atggaaacaa attcatcccc tttcatcata tacaatgttc tatgtccacc
    11521 cgcaaataca acttctttgc cagtggaatc tattcgacag aaacatatct ccataccgtc
    11581 acgcgatgca tcttccttta catcctgtct aagcgcatga cgcacattat tatttaaatg
    11641 ttccagtatc aaagcaggat ctaagatatg ttgctctttt actacttggt ttaaaataga
    11701 actaccaatc attgacatga aggctcccgg gactccatgt cctgtgcaat cgacagccgc
    11761 aataaaggta taaccttcga aatgagtgaa ccagtaaaaa tctccagata caatatcctt
    11821 cggtctaaat aggataaaga aatcttttaa aactgttcct attccagatt tagaaggtag
    11881 aatcgcctct tgaattgttt ttgcataatt aatactatca gtaatgtgtt gattcttttc
    11941 tgctaattgt tcatttgttt cagccaattc tttcgttcta tctctaactt tctgttccat
    12001 attcgcataa agcaaagcat tatcaataga gatagcagct tgggaagata gaacgttaac
    12061 tacttggagc ctatcggctg taaatgctcc actcgacaga ttattttcta aatataatac
    12121 tccagttaat tctccctgtt taataatagg agagcaaaga atagatttga tttgatttct
    12181 agaaatatag gtatctttat taaatttatc atcggttgtt gcattgatta aaactttact
    12241 ttctttcgtt ctctctacgt aataaactac agaactagca atatccggat actcatctac
    12301 tggaatattt gttaatactt tcacatcgtc agacgaaata gatccttcag cttcaatata
    12361 aagtttttta tctttcttta aaagtaaaac tcctctttgc gcgccagcat tttcaaccac
    12421 aatactcatg attttattta acaaattctc aattttgatt tcaccagaga tagcagatga
    12481 acttttcaaa acagattgta aatcaagtgt attcgtcgta tgtagctgtg ttgcagaacc
    12541 actacctgtc tgagtagaaa tactcattgt tccggataat gtcttggaga aatcgtatgg
    12601 tattttctga tctgtgatta gatttgaata tctttccttt agatcttcac attttcgaat
    12661 tgcaccccaa ccttcataac atgtatatgc ttctagaaaa taattttgag caaatctata
    12721 attctttttc ttgagccaaa actttgcagc gagttcattt gctaaggctt cattctgaac
    12781 aaactcattt ttcctagctt ctaaaatgga ctcatcataa tatttcgcag ctttccagtt
    12841 tttatattct agtctagcaa tctctgcttc gaccagtaaa tacttatgta agtaattttc
    12901 aggacaactc tctgcccaga ttttcatttg cttttgattg gttttaattt gattgatata
    12961 ttttattcta tcttctttag aagcatcttt ataaacagct gctagaataa gagaataata
    13021 gaagttatgc tctgcaacag aattaattcc atttataaat ggaatcatct tctctgtttc
    13081 taaaattaag cttaacattt catcatgcat ttcatacaaa aacagcatct gacatttcat
    13141 gatcttatag atcgtgatag gatacatact ttgatgcaaa ttacataaat ctaaatagct
    13201 tttttcatta taagaattgg attgaaaaac atctttacct gatgtctttc cacttgcatt
    13261 ggaaacgata atctctacac ctaagattgt atcgattgcc atcacgtttt ttgcatgaac
    13321 cgaaaagtcc aactgcttag gtaaatcctc catcatacta gaaaatgttt ttccagtgta
    13381 aaaggaattt accataatat ggagtgcact ataaccaccg tgcataaact ccccagaatc
    13441 aagtgcagct tgtattccat cacggttgat tttatcagaa agtatcagat ggtgattcca
    13501 tggattcgca taattagcta gaatgtttgc agccttggca ataccagctt tactttcaaa
    13561 cttttgacta atctgtaaag aaagcaaact ataggaatat gcatttctat attcacctga
    13621 agaagcaagc atgataccat aagctgaata tccatacgac tctggaagat ttccatattt
    13681 taagaatatg tttaccattt tcaaacaaat tacaggaaat agctcaatgg caatattata
    13741 agctgttgta attgtattca tcaaaagctt cacagccatt tccattgttg gattctgcat
    13801 ttttgcttca ttgataagat caggaacttt tctatttcct aaatttttag taacttcttt
    13861 tgattcttct gcaataatat cttgaaaatt ttcacttggc aaagatagac cgagtggttt
    13921 caaagctttt ataacagtag gaagagcttt atcaaatttt ccttcaacag tatattgaat
    13981 cataagcata ttgtatgctt cccctttttc aatatctgtt ttagcatgat caaggataat
    14041 accgattagc ttctcagaat tttcaaaatt accatttaga tattcaacct ctccgagttc
    14101 tttataaaca ataatggaaa gctcatagta tttttcccaa ggttttgaaa attgatttaa
    14161 gattctatga gcctgactta gatactcaag tgcaggttta taagcggtag atatttttgc
    14221 tttttttcca gcttgtaaat taagttcgat tagatttttc ttttcagact catctgtaat
    14281 taaatcagaa ccaatattca aatggttcac tacatcaaat aaattttctt cccatccttc
    14341 attttttagg agaattcttc caatttttaa tcgtagagat ttcttctcct cttgaccaat
    14401 taattcatag cttgcttgct gaactctatc atgctggaaa cgataggcga tttttttcgc
    14461 ggtttcatag tttcttcgca tgtccttgat atctgttccc atcgaatcta ctaatcgata
    14521 attgtctcca accggataga ttaattcttc tttgatcgtt tcgctcaaat ctcttttagt
    14581 atcttgtggc cacgcattgt taattaaaga gagagtggct aattcaaaat tatttccaat
    14641 acaagaagcc aattttaaaa tatgctgagt tttttctgag agtttttgaa tacgcccgat
    14701 gagtaattca actacgtttt cggaaatacg tgtagatcta attttttcaa tatcccattt
    14761 ccattttcca ttttcataat cgaatgaaat tgaatcatct ttggttaatt gctttaacaa
    14821 ctctgtaata aaaaaaggat tcccgccagt tttttgaaat actagttcgg ataaaagttt
    14881 tacatcttct ggctttgtaa agagactatc gcatagtaat tcatttacat gttgttcttc
    14941 gagaggcttt aaataaatag agtcaactac cccaccctct ttttttatcg catctaacat
    15001 tatgacaaat ggatgactgt tgtccgtttc gttgtctcta taagcaagca ttaaaaagag
    15061 atatcgaaca gaggaatcta acattaagtt tttgataaga cctaaagaag cagaatctgc
    15121 ccactgtaaa tcatctaaaa atatagcaag tggatgctca tagttcgcaa atacttttat
    15181 aaaattttga aatactaaat agaaacgatt tgagttttcc tgagccccta attctgcaac
    15241 aggcggctgt tccccaatga taaactctag ctctggaata acgtctgtaa ttactttacc
    15301 attcgatccg agtgctgtta aaatctt
//
LOCUS       DAOQOS010000127        15059 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_2010_length_15059_cov_6.182885, whole genome shotgun sequence.
ACCESSION   DAOQOS010000127 DAOQOS010000000
VERSION     DAOQOS010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 15059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15059
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2010_length_15059_cov_6.182885"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(138..485)
                     /locus_tag="PLF66_20925"
     CDS             complement(138..485)
                     /locus_tag="PLF66_20925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66567.1"
                     /translation="MYPIFFGKYIIILDDSGNLFWVDKEFGEMRHKYVMEEFPVGYPI
                     LKDNIIYFVGRRGNFYAIHLSDATTAWKDLALIGDVFKMYPDKRGIILQGNQSHWLFN
                     YTNGNKQDKMDFK"
     gene            715..1347
                     /locus_tag="PLF66_20930"
     CDS             715..1347
                     /locus_tag="PLF66_20930"
                     /inference="COORDINATES: protein motif:HMM:NF026878.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin type 47 domain-containing
                     protein"
                     /protein_id="HMZ66568.1"
                     /translation="MPTLIKPPTTEQPEVTATSRSNKLLPISMLFGCILIIVASLNYV
                     TKADEEELEALQAKLDKREPLKDYEKEAYCDLLWKVKQIQLCACKKNFIKDIGSLTGY
                     TVQKTDLDWRGSGKTFEEALEEAFRRTGIPKERFVAKKWAKDINGKSGVVEWKGPRSS
                     EISVDAPHEIDGPDVFHIGFKALDLDDQKIKGHIFLDCVPYFRGKKKEDD"
     gene            1340..1828
                     /locus_tag="PLF66_20935"
     CDS             1340..1828
                     /locus_tag="PLF66_20935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66569.1"
                     /translation="MINFVKESLMEALTTTNYTVGEMSPLESIFFHKKVSEKFADGKN
                     YDCLLWEAVGKKSGFSVRDENAWKWLDEFIQLDECILFFEQTDDSTVYTFQENQSLTK
                     FLTKLQGYVFYITNEHLDFLIYFNDSDYLRCIGTAEPWLRNKAKELSKTGWVDMDGKS
                     YL"
     gene            2011..5439
                     /locus_tag="PLF66_20940"
     CDS             2011..5439
                     /locus_tag="PLF66_20940"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HMZ66570.1"
                     /translation="MSDLPPILLDNQSDEKRLSNFLNKTIFPSIQEMYIATGYFYLSG
                     FGVIHPSLLSNPKILDKSSDLIRMIISPKTDKITRDVLDRALSEESEKAKLIQTIIQD
                     FENSLEETTLISAEGLVELLKSKTISIRLFLKDFFHAKAYIAKVNHSGHLHTYTIVGS
                     SNFTLNGLSNNRELNMVENNPIYFDKYKEWFLQLWDNETLPLDATLLQVVEESIRKAK
                     ENQTNHIDSEVFLTPLQMLLFIVKYFLGILSDEDLQVNDQLVEFQRIGAENVMNKLEK
                     LDGAIISDSVGLGKTFTAGEVIKRYIKNNKRVLVITPPNTVLEQWRETLKKYFGIEDG
                     GLLTLYSQGKFSQLENEEVRQIFTKKIDLIVLDEAHRARNKESKLYHNLNSLHPHGER
                     AKVLLLTATPYNNRISDLENLVRLCTTETKLTNAGLTPKSFSDMEEFSKKLFAGIPFS
                     EIENDEKYKKNKMLVKNILNAVMLLRMRTTIREKYGKITIAGKPLVFEDPVVKRMNYT
                     YSDEFQTLFQELPNFLSQLELPHIRISKPGNSGFALGSLYKLFLFKLIESSLYAFNMS
                     IERIITKNLELKERILKDGLSRVMKESQPDIDDSIFSEMEIEEEEDKSQNEDYTEEDV
                     LHWIESDLLLIQKFLDTHIKPHRKDANNPISIHDPKIDKILEILKEPGYKKALVFTTY
                     KDTIFYLKHKLTEEKNAGNFSLAFETATGEDKVNHSLEKKLDRFAPKARNISTENPDF
                     TEINVLFSTDVLSEGVNLQDADLLINFDLPWNPMRIVQRIGRVNRIGSENKIKVINLS
                     PDSTLDQFLNLIKILETKVTMVATLLGKEMAILSSDKETIHAEDIGEEIKKRENISSI
                     SDYERISASNSFFGDLEGETDEDYFRSYLFFSSQENRIRQKDFAEITSFKSTKVYYSI
                     LNQNPKTSYTFYEIYGKRGNYKDLLSRICIKAEYSLDTKQVMVTQNYPFHLFTKENMS
                     KSGFTKFELTNIETFRELEKNTKETFKEILENRTKQYSLASLNQRAKKIKGGQTILYT
                     QIASLLQKDEQELIGESFREVLEKKNCSREIEELLTILALYQLPTNKLKELELALKKV
                     GIVWEKQISKPLYIPFTEELLHFYNTTIMRDPNLRGVLYRKEEIFGEKIVCLAI"
     gene            5424..8621
                     /locus_tag="PLF66_20945"
     CDS             5424..8621
                     /locus_tag="PLF66_20945"
                     /inference="COORDINATES: protein motif:HMM:NF019291.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Eco57I restriction-modification methylase
                     domain-containing protein"
                     /protein_id="HMZ66571.1"
                     /translation="MPSNLNEKQLKEFILAVNSKQEVLDLFKNLGFTVEEGDDSDYVN
                     ISDNDLIEIIFFSSMQDFLNSAKTVSIGKYCTLGISEDFEDWIFAKSVIEDGRKLKKF
                     KFSKTKLSSTPKPIAVKKLLNFKVNDLKSFEDIFKRKDVTKDFYKKFEKLIEALIKKI
                     TGIKSDEDKKWYASVIINRILFIYFIQHKELLGNRDKSYLGKHLKAFSKKKKDSYFQE
                     FLRPLFFEGLSKKDKSPAIQSLLEQVPYLNGGLFQEHILEKENPEIAIPDSIFESIFT
                     FLDEWNWHLDEKRDTEENEINPEVIGYIFEQLINQKQMGAYYTKEDITGYISKNTIIP
                     RLLDMIKEKHPEAFADNPNIFQPIFDNPDAFIYESVLHGYYSDFQKKTIRELPANISA
                     GIKDVSKRTDWNKTADEEYALPTETWREVVARRNRYEEIKSQLQKGLGFDGKSPFTTN
                     DLITYNLNIIQFILHAIENGTFDFVKCFYDSLRSITILDPTCGSGAFLLASLNILEDM
                     YEACLDRLEEFNKVGNGRDRSLQNDRSLRINQLRKYQIYKTIILNNLYGVDIMEEAVE
                     ICKLRLFLKLISQVERNDDLPNLGIEPLPDIDFNILAGNTLVGFSKMSEVEKAFEAGD
                     NYGLAFYASEIDIIKKHASDLEILHHEFIQYQADTTDSPRNPIRTLKEKIQQDAKVLN
                     EKLNLYLAKLYGINVDKKKDLASWIHSHKPFHWVVEFYGILSKGGFDVIVGNPPYVEY
                     SDIAKEYSIIESSMVKTGNLFSIVTARAHEMLNKQSRWSLIIPISSVSTPRMVPLFEY
                     ILKYNKNICVSNYAVRPGKLFVGVDMNLTIIISEFSPSENRENQNIFTTSYLRWASEG
                     RDNLFKLISYCRIGYYNKEGFLLKFGDNISYSIYKKIENLKKLNSYFSEVGDVIFYHS
                     GGRYFRKCIKEKLSNEYKELTIKSGLGDNTIAIMSTSFYYWLWIAVSDAFHVTKKDID
                     VIPVNENFFKDKDVKELSAKLLLDLYKNSTKVDRNRKDGSKQLEINFHVRLSKPIIDE
                     IDRVLAKHYGFTEEELDYIINYDIKYRMGAEGNEE"
     gene            8803..9168
                     /locus_tag="PLF66_20950"
     CDS             8803..9168
                     /locus_tag="PLF66_20950"
                     /inference="COORDINATES: protein motif:HMM:NF016370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease domain-containing protein"
                     /protein_id="HMZ66572.1"
                     /translation="MLPHNKNLKKKARKLRRNMTSAEKMIWYQLLAKDKLEGFRFLRQ
                     KPIDEYIADFYCHKLKLVIEIDGESHLAEEAKDYDEHRTRVLNAYGIEVVRYTNEQIR
                     NHLDEVEKDLKEKVSRRFL"
     gene            9165..11114
                     /locus_tag="PLF66_20955"
     CDS             9165..11114
                     /locus_tag="PLF66_20955"
                     /inference="COORDINATES: protein motif:HMM:NF024573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HMZ66573.1"
                     /translation="MKIESVRIENFRSFKDQTIHFDDYTCFVGPNGAGKSTVFNALNV
                     FFRQFKDSKTDLSKLTESDFHHKNISEDIRITVTFKELSKQAKEDLSDYVRQDKLVVT
                     AAAKYDKDSGRAEVKQYGNRLGFIDFKVFFEAEKEGKKASELKAIFANLKEKYKDLRP
                     ATTREDMANSLQEYEAAHSKQCDLIPSEDQFYGATKGANKLSPHIQWVFVSASKDATE
                     ESEESKTSALGQLLARTVRSKVNFSEKIIELREQVQEQYKALLKAEQSALDDISKSLQ
                     SRLTSWAHPNLTANVLWKEDSEKSIKIEDPFAFIQIGERGFEGDLSRFGHGLQRSYML
                     ALLQELAHYDDKNSSTLIMGIEEPELYQHPPQARYLAETLIDLSEKGTQVFLCTHSPL
                     FIPGDNFDKIRIVRDSGKPSFTAITSLSYKSLSDELKKVGEKHLKESGMVAKLYPSLN
                     PVVNEMFFCKALVLVEGQEDLAYLTSYLFLTDKIYDFRKYGCHIVPVDSKSNLIKPLA
                     MAKLLNIPSYVIFDADTNKDSIPDENKRKSEVGKHKKDNKSILSLQGHDTENEWPSNH
                     IIKENLTCWKTNLTDCIKSEIGEKWESYRNQCSTFYNHASDLEKNPLAIAKTSDLAWA
                     DGQKSKILSDLVDRIIEFGKKSLNV"
     gene            11231..11740
                     /locus_tag="PLF66_20960"
     CDS             11231..11740
                     /locus_tag="PLF66_20960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HMZ66574.1"
                     /translation="MRKHNGMRPQDIAILLKIAAKKDSPWTMKELASELFISASEVSE
                     SLARNVYSGLLQADKRKIMKTDFLNFLKSGLKFVFPAKVTSIGRGMTTAYSAKPLESL
                     IQSSDKVVWSYAKGKEKGMVIEPLIPSIPKACEIDSTFYELIALTDALRIGKVREQNL
                     AFEELNKRL"
     gene            11737..12435
                     /locus_tag="PLF66_20965"
     CDS             11737..12435
                     /locus_tag="PLF66_20965"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HMZ66575.1"
                     /translation="MSILRKNELLIKRIAHALKGINANIVFVGGAVVGFYANDPAADD
                     VRPTVDIDISVEITSNIELEEFRKKINKIGFQHDSKIICRFHYDGIIVDVMGRKEIGW
                     APANKWFDLGFDYLEEKEIDDLKIQILSFPYFLATKFEAYQSRGISDPRSSKDFEDIA
                     YLLDNVTDFPEKIKSANKDVRIYLIERLQEIQNSDTLQEGIISQLSYQTQNERFQMIM
                     EKIESFKRHDKSDS"
     gene            complement(12592..14244)
                     /gene="groL"
                     /locus_tag="PLF66_20970"
     CDS             complement(12592..14244)
                     /gene="groL"
                     /locus_tag="PLF66_20970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004751305.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HMZ66576.1"
                     /translation="MAKVIEYNEEARRKLLAGVNKLANAVKVTLGPKGRNVVIDKKFG
                     SPTITKDGVTVAKEIELEDAIENMGAQMVKEVSTKTNDIAGDGTTTATILAQAIITEG
                     LKNVTAGANPMALKHGIDKAVSAAVDYIKKKAVKIENKQDIANVGTISANNDKEIGNL
                     IADAMDKVGKDGVITVEEAKSIETTLDVVEGMQFDRGYVSPYMVTDPESMTATLNDPF
                     ILIYDKKISSMKDLLPVLEKVAQSGKALLIIAEEVEGEALATIVVNTIRKTISCVAVK
                     APGFGDRRKSMLEDIAVLTGGQVVSEDLGMKLENADLKTLGRAVKVTVDKENTTIIEG
                     KGSSKDIQGRIAQIKKQIEDTTSEYDREKLQERLAKLAGGVAVIHVGAATEVEMKEKK
                     ARVEDALSATRSAVDEGIVAGGGLTLLRAQDAVKSLKLEGDEATGAKIVLRALEEPIR
                     MITNNAGLEGSVIVEEARRKEGNTGFNALTMVWEDMIKAGVVDPAKVVRSALQNAASI
                     GSMILTTEVTITDKPEKEGGAGGGMPGGMGGMGGMGGMGGMM"
     gene            complement(14262..14546)
                     /locus_tag="PLF66_20975"
     CDS             complement(14262..14546)
                     /locus_tag="PLF66_20975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HMZ66577.1"
                     /translation="MAIKPLGDRVLIEPKTEAEEKIGSLYVPDTAKEKQQEGKVIEVG
                     SGRYEDGKLVPVEVKVGDIVLYGKYSGTEVKQGGKEYLIVRESDILAVIK"
     gene            complement(14867..>15059)
                     /locus_tag="PLF66_20980"
     CDS             complement(14867..>15059)
                     /locus_tag="PLF66_20980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66578.1"
                     /translation="KNNYKMIFVFFEARSSYNREFQMLPIFVGAALIEKKMNSVLRNL
                     LRMFHFPMPSAESALYNFK"
BASE COUNT         5232 a         2638 c         2691 g         4498 t
ORIGIN      
        1 tactgaaaaa taggaaatta atttcgacgt tcgatataaa tagtaatgtt gaaacttttt
       61 gttaaataaa aaaaggctgt cttaacttct gccaatacac taaaacattt tttaaaaata
      121 tactttataa aaaaacgtta tttgaaatcc atcttatctt gtttgtttcc gtttgtataa
      181 ttaaagagcc aatgactctg attgccttgt aaaataatcc ctcttttatc tggatacatt
      241 ttgaatacat caccaataag agcaaggtct ttccatgccg tagtagcgtc agagagatgg
      301 attgcgtaaa aatttcctct acgtccaacg aaataaataa tgttgtcttt taaaatagga
      361 tagccaacag gaaattcttc catcacatat ttatgacgca tctcaccaaa ctctttatct
      421 acccagaata aatttccaga atcatctaga atgataatat actttccaaa aaagattgga
      481 tacatgagaa tagcaccggg ggtaagaaaa aaccactgtc tgaactatga tttgtatgat
      541 ttatttgatg actatgattt taatattgaa atatttttgt gtcttaatca caacaaggaa
      601 actttaaata cattttctgc tacattcaaa atttaaaatt cagcatcata atttcttctc
      661 attatccacc aaaatattct tgaatcagta aaatgaaatt gcgaccctga ccgaatgcct
      721 actctaataa aaccaccgac taccgaacaa ccagaagtaa ccgccacttc acggtcaaat
      781 aaactattac ctattagtat gctatttggt tgtatactta ttatcgtcgc ttcactaaac
      841 tatgttacaa aagccgatga agaagagctt gaagcgttac aagccaagct agataaacgt
      901 gaacctctaa aagattatga aaaagaagcc tattgcgacc tattatggaa ggtaaagcag
      961 atacaattat gtgcttgcaa aaagaatttt ataaaagata ttgggagtct gactggctat
     1021 actgttcaga aaaccgattt agattggaga ggttccggaa aaactttcga agaagcatta
     1081 gaggaagcat ttcgtagaac aggtattcct aaagaacgtt ttgtcgcaaa aaaatgggcg
     1141 aaagacatta atggaaaatc aggcgtggtg gaatggaaag gtccaagaag tagcgaaata
     1201 agcgtagatg ctccgcatga aattgatggt ccggacgttt ttcatattgg atttaaagcg
     1261 ttagatttag atgaccaaaa aattaagggg catatttttc tagattgcgt tccctatttc
     1321 agaggaaaaa agaaagaaga tgattaactt tgtaaaggaa agtctcatgg aagcactgac
     1381 gaccactaat tatacggttg gtgaaatgtc tccactcgaa tctattttct ttcataaaaa
     1441 agtttctgaa aaatttgctg atggcaaaaa ctacgattgt ttattgtggg aagctgttgg
     1501 taaaaaatct ggatttagtg tccgcgatga aaatgcatgg aaatggttag atgaatttat
     1561 tcaactagat gaatgcattc tcttctttga acaaaccgat gattcaactg tctatacctt
     1621 tcaagaaaat caatctctta ctaaattcct aaccaaacta caaggttatg ttttttacat
     1681 cacaaacgaa catctagatt tcttaatcta cttcaatgat agcgactatc ttcgttgcat
     1741 cggcaccgcc gagccttggc ttcgcaacaa agccaaagaa ctttccaaaa ccggctgggt
     1801 cgatatggac gggaagagtt atttgtaggc gcgtaagttt tcgcagtcgg aaaaaatcat
     1861 atttgtagtt tcaacttagg cattttgtga aacgaaaagc ctagtttttt gtaggtttgc
     1921 tcaacttttc cattgacagt cttattatca aaaatagttt ctattttgtc ctaacctaat
     1981 ggtaagctag aaaaaaagga gcatctatag atgagtgatt taccacccat attactcgac
     2041 aaccaatccg acgagaaacg attatccaat tttctaaaca agacaatatt tcccagtatt
     2101 caggaaatgt acattgccac cggctacttc tacttatccg gcttcggcgt tattcatcct
     2161 agcttacttt ctaatccaaa gattctagac aaatcatctg atttgattcg aatgattatt
     2221 agtcctaaga ctgacaaaat cacgcgcgat gttttagata gagcattgtc cgaggaaagc
     2281 gagaaggcaa aattaatcca aacgatcatc caagactttg aaaattcttt agaagaaaca
     2341 actcttattt ctgcggaagg tttagttgaa ctactgaaga gcaaaacaat tagtattcgc
     2401 ttatttctaa aagatttttt tcacgcaaag gcatatatcg caaaagtcaa tcatagtggg
     2461 catctgcata cttatacaat cgttggttca tctaatttta ctttaaatgg tctttcgaat
     2521 aatcgcgaat tgaacatggt cgaaaataat ccaatttatt tcgataaata caaagaatgg
     2581 tttttgcagt tgtgggataa tgaaacttta ccgcttgatg caactctttt acaagttgta
     2641 gaagagtcta ttcgaaaagc aaaagagaac caaaccaatc atatagattc agaagtgttc
     2701 ttaactcctc tccaaatgct actttttatc gtcaagtatt tcctaggaat tctaagcgat
     2761 gaagatttac aagtaaacga tcagctagta gagttccaga gaattggtgc tgagaatgta
     2821 atgaacaaac tcgaaaaact agatggtgcg attatctcgg attcagttgg gttaggaaaa
     2881 acgtttactg ctggagaagt cattaagcgt tatatcaaaa ataataaacg agtcctagtc
     2941 atcactccac ctaatactgt cttagagcag tggagagaga ctttgaaaaa atactttggc
     3001 atcgaagatg gcggcttact cacgttatac tcacaaggga aattttccca attagaaaat
     3061 gaggaagtca gacaaatatt cacaaaaaaa attgacctga ttgttttaga tgaagctcat
     3121 agagctagaa ataaagaatc caaactctac cataatctaa acagccttca tccacacgga
     3181 gaacgggcaa aggtgttgct attaaccgca actccataca acaatagaat cagtgattta
     3241 gagaatttag ttcgtctttg cacgacagaa actaaattga ctaacgctgg tttaactccc
     3301 aaatcttttt cggacatgga agaattttct aaaaaattat tcgcaggaat tcctttctca
     3361 gaaatagaaa acgatgaaaa atacaaaaag aacaaaatgc tggttaaaaa tattctaaat
     3421 gcagtcatgc ttcttagaat gagaacaacc attcgagaaa aatacggtaa gattaccatt
     3481 gcaggcaaac ccttagtatt cgaagatcca gttgtaaaaa gaatgaatta tacctattca
     3541 gatgaatttc aaactttatt tcaagaactc ccaaattttc tttctcagtt ggaattgcca
     3601 catattcgga tttctaaacc gggtaattct ggttttgcgc taggaagcct ttacaaattg
     3661 ttcttgttta agctaataga atcaagcctt tatgcgttca atatgtccat tgaaagaatc
     3721 attaccaaaa atttagaact caaagaacgt atcttaaaag atggactttc gcgagtaatg
     3781 aaagagagtc aaccggatat tgacgattct atattttctg aaatggaaat agaagaagag
     3841 gaagataaat cgcaaaacga ggattataca gaagaagatg tacttcattg gattgaaagt
     3901 gatttgttac tcattcaaaa atttttggat acgcatatta aaccacatcg taaagatgct
     3961 aacaatccaa tttcgattca tgacccaaag attgataaaa ttctcgaaat cctaaaagaa
     4021 cccggctaca aaaaagctct ggtgtttact acctacaaag acacaatctt ttacttaaag
     4081 cataaactca cagaagaaaa aaatgcaggt aacttctcgt tagcttttga aacagccaca
     4141 ggtgaggata aggtaaatca ttctctagag aaaaaactag atcgcttcgc gcctaaggca
     4201 agaaacattt ctacagaaaa tcctgatttt actgaaatca atgttctatt ttcgactgac
     4261 gttctttcgg agggtgtgaa cttgcaagat gcagacttac tcattaactt tgatttgcct
     4321 tggaatccaa tgcgtattgt ccaaagaatt ggtcgggtga atcgaattgg ctcagaaaat
     4381 aaaatcaaag taatcaatct aagtcccgat tctacactcg atcaattttt aaatttgatt
     4441 aaaatactag aaacgaaggt gacgatggtg gcgacccttt taggaaaaga aatggcaatt
     4501 cttagctcgg acaaagaaac cattcatgca gaggatattg gagaagaaat caagaaacga
     4561 gaaaacattt ccagtatttc cgattatgaa agaatttctg cttccaattc cttctttggt
     4621 gatttagagg gagaaacgga tgaagattat tttaggagtt acttgttctt ttcctcacag
     4681 gaaaacagaa ttcgtcagaa agattttgcc gaaattactt cttttaaatc gaccaaagtt
     4741 tattattcta ttctaaacca aaaccctaaa acttcttaca cgttctatga aatctatggg
     4801 aaacgtggga attacaaaga tttgctttct agaatttgta taaaagcaga atacagcctt
     4861 gacacaaaac aagttatggt tacacaaaat tatccctttc atctttttac aaaagagaat
     4921 atgtcaaagt ctggatttac taaatttgag ttaacaaata tagaaacatt tcgagaatta
     4981 gaaaaaaata ccaaagaaac ttttaaagaa atcctcgaaa acagaacgaa acaatattcc
     5041 cttgccagct taaaccagag agcaaaaaag attaaaggtg gacaaaccat tctttacacg
     5101 caaatcgcaa gtctacttca aaaagacgaa caagagctca ttggagaatc tttccgcgaa
     5161 gtattagaaa aaaagaattg ttctagagaa atagaagagc ttctaaccat tttagcactc
     5221 taccaacttc ctaccaataa attaaaagaa ttagaattag ctttgaagaa ggttggcatt
     5281 gtttgggaaa aacaaatctc taaaccctta tacattccat tcacagaaga actcctgcat
     5341 ttttataaca caactataat gagagatcca aaccttcgtg gggtattgta tcgaaaagaa
     5401 gaaatctttg gagaaaaaat agtatgccta gcaatctaaa tgaaaaacaa ttaaaagaat
     5461 tcattctagc ggttaacagc aaacaagaag ttctagacct atttaaaaac cttggcttta
     5521 ctgttgaaga aggagacgac tcggactatg taaatatttc tgataatgat ttaattgaga
     5581 tcattttctt ttcctctatg caggattttt taaatagtgc aaaaactgtt tccattggaa
     5641 agtattgcac tcttgggatt tcagaagatt ttgaagattg gatttttgcg aaatcagtga
     5701 ttgaagatgg gcgtaaacta aagaaattca aattctctaa aacaaaacta agtagtactc
     5761 caaaaccaat tgcagtcaaa aaactcttaa atttcaaagt gaatgattta aaatccttcg
     5821 aggacatttt taaacgaaaa gatgtaacca aagattttta caaaaagttt gaaaaactaa
     5881 ttgaagcact cattaaaaaa attacgggaa tcaaatcgga tgaggataag aagtggtatg
     5941 cgtcagtcat catcaatcga attttattta tctactttat ccaacacaaa gaattacttg
     6001 gaaatcgaga taagtcgtat ctaggtaaac atctaaaagc attttccaag aagaaaaaag
     6061 attcttactt tcaagaattt ttgcgcccct tattttttga aggactttcc aagaaggata
     6121 aatctcctgc gatccaaagt ctattagaac aagttcctta tctaaacggt ggacttttcc
     6181 aagaacatat tttagaaaaa gaaaatcctg aaatcgcaat tccagattct atattcgaaa
     6241 gtatctttac ctttttagat gaatggaatt ggcatttaga tgaaaagaga gatacagaag
     6301 aaaatgaaat caatcctgag gtgattggat atatttttga acaactcatc aaccaaaaac
     6361 aaatgggtgc gtattacacc aaggaagata ttactggata catttcaaag aacacaatca
     6421 tcccgcgtct actcgatatg ataaaagaaa aacatccaga agcatttgcg gataatccaa
     6481 atatatttca acctatattc gataacccag acgcttttat ttacgagtct gtattgcacg
     6541 gatactattc cgactttcag aaaaaaacaa ttcgagaatt acccgcaaat atttccgcag
     6601 gaataaaaga tgtctccaaa agaaccgatt ggaataaaac ggctgacgag gagtatgcgc
     6661 ttccaacgga aacttggaga gaagtagtag ccagacgcaa ccgctacgaa gagataaaat
     6721 cccaactcca aaaaggtctt ggctttgacg gtaaatctcc atttaccaca aatgatttaa
     6781 tcacctacaa tttgaatatc atccaattta tcctacatgc cattgaaaat ggaacttttg
     6841 attttgtaaa atgcttttac gatagcctac gctctatcac aattctagac ccaacctgtg
     6901 gctccggtgc attcctatta gcatctctca atatcctaga agacatgtac gaagcttgtt
     6961 tggatagatt ggaagaattc aataaagtag ggaacggtcg cgaccgttcc ctacaaaatg
     7021 accgttctct acgaattaat caattacgaa aataccaaat ctataaaacc atcattctaa
     7081 ataatctcta tggtgtagac atcatggaag aggcagtcga aatctgcaag cttcgcttat
     7141 ttctaaaact catttcccaa gtagagagaa acgacgattt gccgaactta ggaatcgaac
     7201 cacttccaga cattgacttc aatattctag ctggaaatac tctcgtaggc ttctctaaaa
     7261 tgtccgaagt agaaaaagca tttgaagcag gggataatta cggtttagcg ttctacgcaa
     7321 gtgaaatcga catcatcaaa aaacatgcaa gcgacctaga aatcctacat cacgaattta
     7381 tccaatacca agcggatact accgattcac ccagaaatcc tatccgcacc ctcaaagaaa
     7441 aaatccaaca agacgcaaaa gtcctaaacg aaaaactaaa cctctatctc gccaaactct
     7501 acggtatcaa tgtcgataag aaaaaagacc tcgcttcttg gatacattcg cataaaccct
     7561 ttcactgggt cgtcgaattt tacggcatcc tctccaaagg cggcttcgat gtgattgtgg
     7621 gtaatccgcc ttatgtggag tatagtgata tagcgaaaga atactcaata attgaatcaa
     7681 gcatggtcaa aaccggaaat ttattttcaa ttgtaacagc acgagctcat gaaatgttaa
     7741 ataagcaaag tagatggagc ttaattattc caattagttc tgtatctact ccaagaatgg
     7801 ttcctttgtt tgaatacata ttaaagtata acaaaaatat atgcgttagt aactatgccg
     7861 ttcgacctgg aaaattgttt gttggggtag atatgaattt aacgattatt atttctgagt
     7921 ttagcccaag tgaaaataga gaaaatcaga atatttttac gacgagttat cttcggtggg
     7981 caagcgaagg aagggataat ctttttaaac taatctcata ctgtagaata ggttattata
     8041 ataaagaagg atttctttta aagtttgggg ataatatatc ttattcaatt tataaaaaaa
     8101 ttgagaatct aaaaaaattg aactcttatt tttctgaagt cggggatgta attttctatc
     8161 atagtggtgg gcgatatttt agaaaatgca taaaagaaaa attatctaac gaatataaag
     8221 aactcacgat aaaaagtggg ctcggagata acactattgc gataatgtcg acttcttttt
     8281 attattggct ctggatcgca gtttcagatg cgttccatgt tacaaaaaaa gatattgatg
     8341 tcattccggt aaatgagaat ttctttaagg acaaagatgt aaaagaatta tctgctaaac
     8401 ttttattaga tttatataaa aattcaacga aagtagacag aaaccgaaag gatggtagca
     8461 aacagttaga gatcaacttt catgtacgac tctccaaacc catcatcgac gaaatcgacc
     8521 gagtattggc gaagcattat ggttttacgg aagaggagtt ggactatata atcaactatg
     8581 atataaagta tagaatgggt gcggagggta atgaggagta atccccccta cccccctttg
     8641 ggaaaggggg ttttgttcta aggtttatct tctgtatttc ctaaacctat tttacgaaaa
     8701 agtaccacaa ctcgtaaacc ccctttccca aagggggatc aagggggatt cgaagaacgc
     8761 aaagtaaatc ttagaacaaa aaaatagagg ataacaaaga ctatgctacc acacaacaaa
     8821 aatctgaaaa aaaaagcacg caagttaaga cggaatatga caagtgcaga gaaaatgatt
     8881 tggtatcaac ttttagcaaa ggacaaacta gaaggattca gatttttacg acaaaagcca
     8941 attgatgagt atatagcaga tttctattgt cataagttaa aactagtaat tgaaatcgac
     9001 ggcgaatcgc acttagccga agaagcaaag gattatgacg agcatcgaac tagagtgtta
     9061 aatgcctatg gaattgaagt agtgcgatat acgaatgagc agatacgcaa tcatttagac
     9121 gaagtagaaa aagatttaaa agagaaagtg agtaggagat ttttatgaaa atagaatcag
     9181 ttaggataga aaattttaga tcatttaaag atcagacaat tcattttgat gattatactt
     9241 gttttgttgg tccaaatggt gcaggaaaat caacagtatt caatgcacta aatgttttct
     9301 ttcgacaatt caaagatagc aaaacagatt tgagtaaact aactgaaagt gactttcatc
     9361 ataaaaatat ttccgaagat attcgaatta cagttacctt caaagaatta tctaagcaag
     9421 caaaagaaga tctttctgat tatgttagac aagataaact ggtcgtaacc gcagcagcaa
     9481 agtatgacaa agatagcgga cgagcagaag taaaacaata tggcaaccgt ttaggattta
     9541 tcgattttaa agtattcttt gaagcggaga aagaaggaaa aaaagcctca gagctaaaag
     9601 caatttttgc aaacttaaaa gagaaatata aagatttaag accggcaacg acaagagagg
     9661 atatggcaaa tagtcttcag gagtatgaag cagcgcattc aaagcagtgt gatttaatac
     9721 ctagtgaaga tcaattttat ggagccacaa aaggggcaaa taagctttcg ccgcacattc
     9781 aatgggtttt tgtttccgca tccaaagacg ctactgaaga aagtgaagag tcaaaaacat
     9841 ctgcgttagg acaattactt gcaagaacag taagatcaaa ggtcaatttt agtgagaaaa
     9901 ttattgaatt aagagagcaa gtgcaagagc aatataaagc tttattaaaa gcagagcagt
     9961 cagcattaga tgatatttcc aaatcgttac agagtagatt aacctcttgg gctcacccaa
    10021 acttaacagc caatgttctt tggaaagagg attccgaaaa atcaattaaa atagaagacc
    10081 cttttgcatt tattcaaata ggagaacggg gatttgaagg ggatttgtcc cgatttggtc
    10141 atggattgca gcgatcctat atgttagctt tattacaaga gcttgcgcat tatgatgata
    10201 aaaattcttc gactcttatt atgggaattg aagaaccgga gttataccaa catccaccgc
    10261 aagcaagata tttggcagaa acgttaatcg acctctcaga aaaaggaaca caagtttttt
    10321 tatgcactca tagtccgttg tttattcctg gcgataattt tgataagata agaattgtta
    10381 gagatagtgg caaaccaagt tttaccgcaa ttacttcctt atcttacaaa tctctctccg
    10441 atgaattaaa aaaagtggga gaaaagcatt taaaagaatc gggtatggtt gcaaaattat
    10501 atccttcgct aaatccagtt gtcaatgaaa tgtttttctg caaggcatta gtattagtag
    10561 aggggcaaga agatcttgct tatttaacat cttacttatt tttaactgat aaaatttatg
    10621 atttcagaaa atacggctgt catattgttc ctgttgatag caaaagcaat ttaattaagc
    10681 ctttagcaat ggctaaatta ttaaatattc cttcttatgt tatttttgat gcagatacaa
    10741 acaaggattc gattcctgat gaaaacaaaa gaaagtcaga agtaggaaaa cataaaaagg
    10801 ataataaatc tattctctca ctacaaggtc atgatacaga aaatgaatgg ccttctaacc
    10861 atataataaa agaaaattta acatgttgga aaactaattt aactgattgc ataaagtctg
    10921 aaattggtga gaaatgggaa agttatagaa atcaatgttc tactttttat aatcatgcta
    10981 gtgatctaga aaaaaatcca ttggcaattg caaaaacatc agatttagca tgggcagatg
    11041 gacaaaagtc gaaaattcta tctgatttag ttgatagaat tattgaattt gggaaaaaat
    11101 ctttaaacgt ataagactac ttcaaaccta tcgaactcga taggtttgaa attcggtttg
    11161 tattaatgaa tattacaacc tcctcttaaa attaatttgc aatttgcgaa ttgcgaatta
    11221 agatggtagc atgagaaagc ataacggaat gagaccacaa gacatcgcaa tcctcttaaa
    11281 aatagctgca aagaaggatt caccttggac tatgaaagaa cttgcctctg agctttttat
    11341 tagtgctagt gaggtaagcg agtccttagc tagaaatgtt tattcaggtc tgttacaagc
    11401 agataaaaga aaaatcatga aaacagactt tctgaacttt ctcaagtctg gattaaaatt
    11461 tgtatttcct gcaaaagtga ctagtattgg tagaggaatg acaacggctt acagtgcaaa
    11521 accgttggaa agtttaattc aaagttctga taaggttgtt tggtcttatg cgaagggaaa
    11581 agaaaaagga atggttattg aaccattaat tcctagtatc ccgaaagctt gcgaaattga
    11641 ttctactttc tacgaactca tagcactgac ggatgctttg cgcattggaa aagtaagaga
    11701 acaaaacctg gcttttgagg agttaaataa aagactatga gtatcttacg aaaaaacgaa
    11761 ctgcttataa aaagaatagc tcacgcactg aagggcataa atgcaaacat tgttttcgtt
    11821 ggcggtgcag ttgttggctt ctacgcaaat gatcctgccg ctgatgacgt tagacccaca
    11881 gtagatattg atattagtgt agaaatcaca tccaatatag aattggaaga atttagaaag
    11941 aaaataaata aaatcggatt tcaacatgat tcaaaaatta tttgtaggtt tcactatgat
    12001 ggaatcattg ttgatgtcat gggaagaaaa gagataggtt gggcacctgc aaataaatgg
    12061 tttgatctcg gatttgatta tttagaagaa aaagaaatcg acgatttgaa aatacaaatt
    12121 ttgtctttcc cctactttct tgcgactaaa tttgaagcat atcaaagtcg aggaatttcc
    12181 gatccaagaa gtagtaaaga ctttgaggat attgcctatt tacttgataa cgtaactgat
    12241 ttccctgaaa aaattaaaag tgctaataag gatgtaagga tttatttaat tgaaagactt
    12301 caagaaattc agaatagcga tactttgcaa gaagggatca taagccaact ttcttatcaa
    12361 actcaaaatg aaagatttca aatgataatg gaaaagattg aaagttttaa acgccacgac
    12421 aaatcagaca gttaataaat tgtatgacta ataaagagta ttccattgga gagtgagtta
    12481 agaggatcac cccaaacaca caaaaaaagc cccactgcaa ccagtgaggc ttcgcttcgc
    12541 ggataacgta tggacttgat tcatcaagtc catacaacac gaacgaaatg atcacatcat
    12601 tccgcccatg cctcccatac cacccattcc gcccatacca ccaggcattc caccaccagc
    12661 accaccttct ttctcaggtt tgtcagtgat agtaacctca gttgtgagga tcatagaacc
    12721 gatagacgct gcattttgga gagcagaacg aactacttta gcaggatcaa caacacccgc
    12781 tttgatcatg tcttcccaaa ccatagtaag agcgttaaat ccagtgttac cttcttttct
    12841 tctagcttct tctacgatta cggaaccttc tagaccagcg ttgtttgtga tcattctgat
    12901 tggctcttct agagcgcgta gaacgatttt tgctccagtt gcttcgtcgc cttctagttt
    12961 gagagattta acagcatctt gagcacggag gagggttaaa ccaccaccag ctacgatacc
    13021 ttcatctaca gcagaacgag tagcagataa cgcatcttct acgcgagctt ttttctcttt
    13081 catttctact tcagttgcag cacctacatg aattacagca accccgccag cgagtttagc
    13141 gagtctttct tggagttttt ctctgtcgta ttcagaagtt gtatcttcga tttgtttttt
    13201 gatttgagca attcttcctt ggatgtcttt agaagaacct tttccttcga tgatcgttgt
    13261 attttctttg tcaacagtta cttttacagc gcgaccaaga gttttgagat cagcgttttc
    13321 caatttcatt ccgaggtctt cagaaacaac ttgtccacca gtaagaactg cgatatcttc
    13381 taacatagat tttcttctgt cgccaaagcc tggagctttc acagcaacac aagagatagt
    13441 ttttctgata gtgtttacaa cgatagttgc aagagcttcc ccttctactt cttctgcaat
    13501 gattaacaat gctttaccgc tttgtgcaac tttttcgagc actgggagaa gatctttcat
    13561 agaagagatt tttttgtcat aaattaaaat gaatggatca tttaaagtag cagtcatgct
    13621 ttctggatct gttaccatgt atggagaaac atatcctctg tcaaattgca taccttctac
    13681 tacatcgagt gtagtttcga tagatttagc ttcttcaaca gtgatgactc cgtctttacc
    13741 tactttgtcc attgcatcag cgattaggtt accgatttct ttgtcgttgt ttgcagaaat
    13801 agttcctacg tttgcaatgt cttgtttgtt ttcgatttta actgctttct ttttgatata
    13861 atcaacagca gcagaaactg ctttgtcgat accatgcttg agagccattg ggttcgcacc
    13921 cgctgttacg ttttttaaac cttctgtgat gatcgcttgt gcaaggatag tagctgttgt
    13981 agtaccgtca cctgctatgt cattggtctt tgtagaaact tctttgacca tttgagcgcc
    14041 catgttttca attgcatctt ctagttcgat ttcttttgct acggttacac cgtcttttgt
    14101 gatagttggt gatccgaatt ttttatcgat cactacatta cgtccttttg gaccaagtgt
    14161 tacttttaca gcgtttgcaa gtttatttac gcctgctaat aattttctgc gtgcttcttc
    14221 gttgtattcg attacttttg ccatttctta tttacccttc cttatttaat tactgctaga
    14281 atgtcactct ctcttacgat taagtattct ttgccgcctt gtttaacttc tgttccggaa
    14341 tattttccgt agagaacgat atcccctact tttacttcaa caggaactaa tttcccgtct
    14401 tcgtatcttc cagatcctac ttcgatcact tttccttctt gctgcttttc ttttgcagta
    14461 tcaggtacgt ataaacttcc aattttttct tcagcctctg tcttaggctc tattaaaact
    14521 ctatcgccaa gtggtttgat agccatgtct atgtatctcc tttgtcttgt gatttagcac
    14581 ttcttaaaac agagtgctaa tttatgttgc catactattc tatagacggt tcaaagtcaa
    14641 gcacttaaag gtcgagagtg ctaaaattcg aaaatttttc gttttttgtg ggatttttgg
    14701 aaagattttt tatataaatt aacacgaatt cgacgcaaca atcttttcat caccgattat
    14761 cacagataac ggaatgataa aaagcgatgg atttatattt tggtgaaaag gctgaaagga
    14821 tgaatatttg aatctttaga ggctaaaacg caatttaatc aacgagtcat ttaaaattat
    14881 acaatgcact ttccgcacta ggcatcggaa aatgaaacat cctcagtagg ttccgcaaaa
    14941 cagaattcat ctttttctct attaaagcgg cgcccacaaa gataggtagc atttgaaatt
    15001 ccctgttata ggaagatctt gcctcaaaga aaacaaagat cattttataa ttattctta
//
LOCUS       DAOQOS010000128        14908 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_2039_length_14908_cov_5.945937, whole genome shotgun sequence.
ACCESSION   DAOQOS010000128 DAOQOS010000000
VERSION     DAOQOS010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 14908)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14908)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14908
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2039_length_14908_cov_5.945937"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..952
                     /locus_tag="PLF66_20985"
     CDS             <1..952
                     /locus_tag="PLF66_20985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66579.1"
                     /translation="DPDGKIWSMNDGNSGVDNINSACNNAGVSSVTIIQDGASGASDT
                     DYASLIKHLCVGKGHHKAIFTTSPKRAFVSSITDNTVSVIDNEPLNSTYLTVLATLSL
                     GTGAGPHGFSYSSVSGKVYISAQTAGTVIAVNPSSLAMETISTPKSGPSTSSPNGLYI
                     ALPGKDTTSDSNHVIGKVTILNASTHVFSTVSITDVSPDHMMFNSDGTRLYVASAQAG
                     SGNQVTNLNQSVLLVYDSSNLPSLNLIAQIPVGACKSGNRHINAASHGSMMHIFNPAG
                     DGILYTVDSMSNSVTQKINLGGSFNNAYYHTLGAAPSHSH"
     gene            1009..1425
                     /locus_tag="PLF66_20990"
     CDS             1009..1425
                     /locus_tag="PLF66_20990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66580.1"
                     /translation="MKIWIIGFMLLSMACTSKSDDSGKKIAALAVISQSRRTTSTTSS
                     SCTANYNCATAPSFSTLATAGTTASCALSGCHTTSSQQSGLDITDFNSARAFTNPGNP
                     CSSRMYTAITTGAMVGNTNARITEAVYCWIAGGSNR"
     gene            1443..2852
                     /locus_tag="PLF66_20995"
     CDS             1443..2852
                     /locus_tag="PLF66_20995"
                     /inference="COORDINATES: protein motif:HMM:NF024946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HMZ66581.1"
                     /translation="MLTHIQRIAILFVFLIPFLLNGQSHKDHGKNTKQEKTSPAKNSE
                     SPKEGKETILTSEKENLKADEKERREVTESKDSESPKTTEKNAESQITNSTPSEKKED
                     REAANANQPHEHSGPSGIPAGLMFPQVHKSNDWMLDYMYMQMDMNQLYNGSNRINAGN
                     SLFGIELNPTVGTIPANQLSTGSVHNHSGTNTNTTPPEALPSYYIASLYPNSYRYMSM
                     PVSMKMEMTMISLMKNINDKLSVMFMIPYMNYSMQSLSGNLEKSFMRTQGVGDIGASL
                     AYKLIEKEAHTFQLQFGVTLPTGSIDEKNLMPLMGRVRSPYNMQLGTGTYNSIPGFNY
                     TYQKGKFTFGSMGQVTLRNGKNDNGYRFGNRYELSVWTSYRLLESLAPTIRITSLKWD
                     NISGSDPSLDPTMDPQNDPNRQGGRRIDLLAGLNFYIPSLIEKVKAGLEFGKPVYQHL
                     NGPQLGTNTLFHFRFQASF"
     gene            3167..3796
                     /locus_tag="PLF66_21000"
     CDS             3167..3796
                     /locus_tag="PLF66_21000"
                     /inference="COORDINATES: protein motif:HMM:NF015724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HMZ66582.1"
                     /translation="MNFKLNVSILLSVLFLLVQCSTIDVAVTGNKQVLKNVKKIAVIN
                     FEMSNRDSREQEYADILGHQFLKSTHFNVIERDKLVISKVIGEQTLSRSGIIDESTAA
                     QMGKILGVDAIVIAKGEPLMIDNKNVDYSLNTFNIKVINVETGNIFVNAIKEPGIAWT
                     PLTRIKYILGLGLIWNKQDMLLETSKINFLAETAVKNIQSEIDSLNQKP"
     gene            3808..4551
                     /locus_tag="PLF66_21005"
     CDS             3808..4551
                     /locus_tag="PLF66_21005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020778163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HMZ66583.1"
                     /translation="MVKTQKVLIIAATSDIGKPIAESFARRGYELYLTSTNVNMLHPF
                     AESISKKFNVDVRDYQFDISDYSIHAQFLKSLPEIPDVVVCCAGYYKDQEKAISDFGE
                     TYRTLSVNYVGVVSILNLISIEFQKRKSGSIIVLSSVAGIRGRQMNFLYGSAKAGITT
                     YLSGLRNRLFKDNVSVTTILLGPVYTKMSEGHNLMPVLTAKPEVAAEKIVKAGLSKKG
                     EVYILWYWKWVMFIIQLIPEFIFKRLKPF"
     gene            complement(4548..4685)
                     /locus_tag="PLF66_21010"
     CDS             complement(4548..4685)
                     /locus_tag="PLF66_21010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66584.1"
                     /translation="MDTDKETTIQNYQSFLYRSVIGVLGGNLINSLLLQTDVSYSSKS
                     N"
     gene            complement(4702..6489)
                     /locus_tag="PLF66_21015"
     CDS             complement(4702..6489)
                     /locus_tag="PLF66_21015"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HMZ66585.1"
                     /translation="MKQILLFGLIALIPLFAEDRTRENILEPNKFETPRETFAVFLKS
                     MNDYKKGLESKNSQLIAELDTAVRCLNLDGIPFGLRAEKGRESAILLKEVIDRIDVID
                     PLTIPDKSENPNDPLTKWIFGNTEISISKVEGGDRANEFLFSRDTVARAFEFYTKTKH
                     LPYLANSGYGAGYKDPWSESLPGWMKFKFFSLYVWQIIGIILSILLGFLVGKITRSIS
                     HLITHIVNRVPEGKINWKEKILTFCDKPLAYIVIVGFWYICLDFLDIDGTPFKISNII
                     LQILLSFNLVRLFYDLTKAFTEYVEMIAKQNKSEFLIDAQIIPLASRTVRILVVILGS
                     LFAMQNLGVNVMSVLAGLGVGGLALALAAKDTAANLFGSIMIFLDRPFKIGDTIILGG
                     IEGDVEEIGFRCTRIRTFYDSVVSIPNSEVANSKIDNMGLRRYRRTNTTLGLTYDTPP
                     EKIEAFLEGIKNILRKNELVVQDRIHVIFKGFGNSSLDILLNYFATVTEWGQDMILRQ
                     NVYLEIINLAKELNISFAFPTQTLHVETLAGQTPIKRDHRESTEDLKQTAKNFAKDGK
                     SSRPKGFGIFIHPSREGKQTDIGVGTTEG"
     gene            6734..7195
                     /locus_tag="PLF66_21020"
     CDS             6734..7195
                     /locus_tag="PLF66_21020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010573533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HMZ66586.1"
                     /translation="MKYWLFKSEPNVFSIEDLKNAKNSTSSWEGIRNFQARNFLRDEA
                     KKGDLVLFYHSSVEPMAVVGVAEVVKEGYIDHFAFDKKSDYYDPKSNPDKPQWYMVDV
                     KFKKEFKRPVTLKEIKTYKELSKMRLVQRGARLSIQPITKEEFDFIVRLGG"
     gene            7559..8689
                     /locus_tag="PLF66_21025"
     CDS             7559..8689
                     /locus_tag="PLF66_21025"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HMZ66587.1"
                     /translation="MNLKSIAIFPRGIILFILLSLILQCSTKDVKKTNNRRMIGIDAN
                     LSLTENVFQKAKTVNEEIFARQDVSDVRTVTVRDLLDKLQLKKDEYCDNKIKDLDDFP
                     VDPANMCCDLNFMNIDPRKGKVHYGFDIPLAIGTPLKCPDKEDCLVVRVYPEWITSDG
                     MPKQTIYGESLLVYFKKAGYFGFYAHLKKIYGRPGRIFNYKEVMALSGNSGSLSTGAH
                     LHFTLYRNQDCSNGVFDEKMRLAVLDTIEEIKIFEAKEKGLDPSSYNIEEKLADDVLK
                     RCKVKMSQEANRACIVRNFPRDFIDPLEVTSDHTIQRLVLEINPKRMRDRDVLRLFSY
                     HQRVRNNSGIHSEISKVSNTTPTNVENLKDLLRNVNILGEEM"
     gene            complement(9053..9961)
                     /locus_tag="PLF66_21030"
     CDS             complement(9053..9961)
                     /locus_tag="PLF66_21030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66588.1"
                     /translation="MIFEAISFSFNLGVFTCLFWNFFSFQDKKLVEKLKRLPLFIAFG
                     FLFYSIFYISTLFSTRFFELKSIYFNAYTILFFLLAVSLLLYLAKSFSLAASLASLLI
                     APYIFFLLFGFEEFTFSENKIYSYSLLKTQNPMIGLISLCLILIKPVKIQIPLFAIAV
                     GYLFYIILPLKEFPYPEIKILHYKYIPYEFFMEEKSYLFEENSGIYTEKKEGVDDKLY
                     KFSMKPLKKINLPLRQTIQYLISSFDFPILLSDENSITLIEMSRSYNDTFFKVVFHLN
                     KNPVGIEVIGNEQIDSATIEFEGPLF"
     gene            10034..>10428
                     /locus_tag="PLF66_21035"
     CDS             10034..>10428
                     /locus_tag="PLF66_21035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004429176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HMZ66589.1"
                     /translation="MDLIEVKISDITLTNVGFAVFLKPKEDLFDSKVVPIFIGPLETH
                     AITSVLDGITPPRPMTHDLIMMIIPVLGAQIIKVTIDEIIDNTFYAKISLRKDEEIFL
                     IDARPSDSIAIALRAEAPIFITKSVLDEAG"
     assembly_gap    10429..10443
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <10444..10627
                     /locus_tag="PLF66_21040"
     CDS             <10444..10627
                     /locus_tag="PLF66_21040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004429176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HMZ66590.1"
                     /translation="MRGGEIPSEPSIEEALSSEPNPPKTQLQLLEDSLAAAIKSEDYE
                     TAARIRDQIKKIIESS"
     gene            10725..11096
                     /locus_tag="PLF66_21045"
     CDS             10725..11096
                     /locus_tag="PLF66_21045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012389269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66591.1"
                     /translation="MEKVLTDVVNAGIALFKTGEEVLKNSLVDFEKKFNEIKQKGELD
                     QSEQAVQVRDLLNKTIKDTKDVLDNANASYQDIVSKAQTNFEGIYNQVDAALPEQVKT
                     QAKAALDELKAVIEKYKPAAK"
     gene            11175..12458
                     /locus_tag="PLF66_21050"
     CDS             11175..12458
                     /locus_tag="PLF66_21050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010575030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HMZ66592.1"
                     /translation="MTYKREKLVLFLTVFIDMMGFSVIFPIFPETLRFFLSREKDPIL
                     NFFIHILNLFNTNSDHRLFIVLFGGFVGSIYSILQFFFAPIWGRLSDKVGRKPALVLT
                     STGNFLGYVVWFFSGSFTLFVISRLITGCMGGNISVASAAMADVTSEKDRAKGMGMIG
                     AGIGLGFMFGPPLGGILASVDLTQIFPALTEYGVTVFSSSALLSVLIALVNLLLVLFV
                     FRETFQPTKEPTKRELHPILGLRTATVQELPLLCLIYLIFSLSFSGFEFCINFFLYDL
                     FQFNPREIGYTFVYLGSIIILIQGGVIRRISGKVAEKKIAMTGAVTLLFGLTALSFSK
                     SVGLAIFSLGFISMGSAFLNPGLSSLASLFSPPQDQGRNLGIMRGFGALARGVSPVTF
                     SLLYFTFGAYSTFAVSAVLILIVIGMIRYVPEKSK"
     gene            12878..13060
                     /locus_tag="PLF66_21055"
     CDS             12878..13060
                     /locus_tag="PLF66_21055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66593.1"
                     /translation="MDEKIKQEVEDGVRCPDCLEVRAIQFYDTEPTVSRLLQNHNKGL
                     VSFYFAKIHSDWSNLH"
     gene            13302..13568
                     /locus_tag="PLF66_21060"
     CDS             13302..13568
                     /locus_tag="PLF66_21060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66594.1"
                     /translation="MPISDDDLKAWQEKIEWETETKTISDYFFVTQCQTCKHFNQWSR
                     KKLRCTAYQDLIPLKIFYGKHDHHFPYPGDEGILYEPMETDNTN"
     gene            13736..14230
                     /locus_tag="PLF66_21065"
     CDS             13736..14230
                     /locus_tag="PLF66_21065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66595.1"
                     /translation="MKYINILSLIINCNLKFLIFVPIITIKILADDFILKAEYANKEY
                     RCRIEESAHKTKKSFPFHKFRLGCTFFQEEMIGYQSGSLMGVIVHKNKGEYLKWMERG
                     NQSGFLPMTNNCFISVSHWKAINPLDFKKYKLSPEKYEKYRINFLDVFEMIRKDIANT
                     EFCR"
     gene            complement(14314..>14908)
                     /locus_tag="PLF66_21070"
     CDS             complement(14314..>14908)
                     /locus_tag="PLF66_21070"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HMZ66596.1"
                     /translation="SDLELFLNNTDLKIPHLIRIAIGHYQFETIHPFLDGNGRIGRLL
                     ITLYLVSSGILDKPLLYLSDFFERNKTLYYDNLTFVRTKNDLTQWIKFFLVGVIETAE
                     KSAITLKKIIDLKAMIEKNKIIKIGKRAPTAHALLHGLFKKPLVTSKDVQSITGLSQK
                     AANELVKVFMKEEILIESTGYKRNKVFVFREYMNMFL"
BASE COUNT         4982 a         2417 c         2795 g         4699 t
ORIGIN      
        1 agatccggat ggaaagatct ggtcaatgaa tgatggaaat tcgggagttg ataatattaa
       61 ttcggcatgc aataatgctg gagttagttc tgtaacaatc attcaggatg gagcgtcagg
      121 cgcaagtgat actgattatg catcccttat taaacatcta tgcgtcggaa aaggtcatca
      181 caaagcaatc tttacaacaa gtccaaagag agcttttgtg agtagtataa ccgataatac
      241 tgtatctgtg atcgataatg agcctttgaa cagcacttat cttacagtgc ttgctacttt
      301 aagtttagga acaggagcgg gacctcatgg gtttagctac tcttctgtat ctggtaaagt
      361 ttatatttct gctcaaactg ctggaacggt catagcagta aatccttcta gccttgctat
      421 ggaaactatt tcgactccta agtcaggacc ttctacttct tctcctaacg gtttgtatat
      481 agcactaccc ggaaaagata caactagtga ttcaaatcat gtgataggaa aggtgacaat
      541 cctaaatgca tctactcatg ttttttcaac agtgagtata acagatgtta gccctgatca
      601 tatgatgttt aattcagatg gaacaaggct ctatgttgct tctgcacaag cgggatcagg
      661 aaatcaggtt acaaacctaa atcaaagtgt attgcttgtg tatgattctt ctaatcttcc
      721 ttctttaaat ctaattgcac aaattcctgt tggggcttgt aagtctggaa atagacatat
      781 aaatgcagca tctcatggtt ctatgatgca tatatttaat cctgcgggag atggaatctt
      841 atatacagtt gattcaatgt caaactcagt tacacaaaag atcaacttag gtggaagctt
      901 taacaatgcg tattatcata cgttaggtgc ggctccctct cattctcatt gaaagaatgc
      961 tgaattctaa aatgtaattt ttatgaaaca aatattaagg aagaaaatat gaaaatttgg
     1021 ataattggat tcatgctttt aagtatggct tgcacaagta aatcggatga ttcaggaaaa
     1081 aaaattgcag cacttgcagt aattagtcaa tctagaagga caacctcaac tacttcaagc
     1141 tcttgcacag caaattataa ttgtgcaacg gctccaagct ttagcactct agctactgct
     1201 ggaactactg cgagttgtgc tttatctgga tgccatacca cttcttctca acaatctgga
     1261 ctagatatta cggattttaa ttcagcaagg gcatttacaa atccaggcaa tccttgttct
     1321 agcagaatgt atacagccat aacgacaggc gcaatggttg ggaatacaaa tgctagaatt
     1381 actgaagcgg tttattgttg gattgccggt ggatcaaacc gctaaattca taaggagaaa
     1441 ttatgttaac ccatatacag cgaatagcta ttctattcgt ttttttaatt ccatttttat
     1501 taaatggtca atctcataaa gatcatggta aaaatactaa gcaggaaaaa acaagtcctg
     1561 ctaaaaattc agaaagccca aaagagggaa aagaaacaat tcttacatcg gagaaagaga
     1621 atttaaaagc agatgaaaaa gaaagaaggg aagtaacaga atcaaaagac tcggaatccc
     1681 cgaaaacaac tgaaaaaaat gcagagtctc aaataacaaa ttccacacct tctgaaaaaa
     1741 aagaagatag agaagcggct aatgctaatc aaccgcatga acattcagga cctagcggaa
     1801 ttcctgctgg tcttatgttt ccacaagtgc ataaatcgaa tgactggatg cttgattata
     1861 tgtatatgca aatggatatg aatcagttat acaatggtag taatcgaatc aatgctggaa
     1921 attctctttt cggaatcgaa ttgaatccaa cagtaggaac cataccggca aaccaactat
     1981 cgactggttc tgtgcataat cattccggaa caaacacaaa tacgactcca ccggaagcac
     2041 ttccatccta ctatattgcc tctctctatc cgaattcgta tcgatatatg tcgatgcctg
     2101 tttcgatgaa gatggagatg acgatgatta gcctcatgaa aaatataaat gataaattat
     2161 ctgttatgtt tatgattcct tatatgaatt atagtatgca atctctatct ggaaatttag
     2221 aaaaatcctt tatgcgaaca cagggggtag gagatattgg agcaagtctt gcttataaat
     2281 tgattgagaa agaagctcat acctttcaat tgcaatttgg agttactttg cctacaggtt
     2341 ctatcgatga aaaaaatcta atgccactta tgggaagagt caggtctccc tataatatgc
     2401 agctcgggac gggaacatac aattcaattc caggatttaa ttatacctac caaaaaggaa
     2461 aatttacttt cggaagtatg ggacaagtta ctcttagaaa tggaaaaaat gacaatggct
     2521 atcgatttgg gaatcgttat gaactatcgg tctggacttc ctatcgatta ttagaatcac
     2581 ttgctccgac aattcgtata acgtctctaa aatgggacaa tatctctggt tctgatccaa
     2641 gcttagatcc tacaatggat ccgcaaaatg atccaaatcg acagggagga agaagaattg
     2701 atttactggc tggactgaat ttttatattc cttctctcat tgagaaagtg aaggcaggat
     2761 tggagtttgg taaacccgtc tatcagcatt taaatggacc acaacttgga accaatactt
     2821 tatttcattt tcgattccaa gcttcttttt aactgctaga atcactccta gagaaaatcc
     2881 ccacttaggt agaggttggg attttccaaa aaatattgga atcgatatgt ttgaccctat
     2941 cttgtagttc tagaaatttt atttacatca actcattcca tctagaatgc ccaataggga
     3001 ctaggatcaa agggaattgc gcaactacta aaagagagat tataaattgg gttaccgatg
     3061 ggaatttctc ttggagaaaa taaaatttag gttggcgata aaaataacca tagcattgga
     3121 aagataaatt catttactaa attaacttat aaaagagagt tgcattatga attttaaatt
     3181 aaatgtatct attctcctga gtgttttatt tttactcgtg caatgttcta cgattgacgt
     3241 agcggttaca ggcaacaaac aagttcttaa gaacgttaaa aaaatagcag tgattaattt
     3301 tgaaatgagc aatcgcgatt ctagagaaca agaatatgct gatatactag gtcaccaatt
     3361 tttaaaaagc acacatttta acgtaatcga aagagacaaa ttagtgatta gtaaagtaat
     3421 tggggagcaa accctttccc gtagtggtat tatagatgaa tcaactgctg cacaaatggg
     3481 aaagatttta ggtgtagatg ctatcgtgat cgcaaaaggg gaacctctta tgattgacaa
     3541 taaaaacgta gattactcgc ttaatacatt taatataaaa gtaatcaatg tagagactgg
     3601 aaatattttt gtaaatgcaa tcaaagaacc tggaatcgct tggactcctt taacaagaat
     3661 caaatatata cttggacttg gtcttatctg gaataaacag gatatgctcc tagaaacatc
     3721 caagattaat ttcttagcag aaacagctgt taaaaatatt caatccgaaa ttgatagttt
     3781 gaatcaaaag ccatagagcg ttttatcatg gttaaaacac aaaaagtatt aattatagcg
     3841 gcaacttcag atataggtaa gcctatcgca gagagttttg ctagaagagg atatgaactt
     3901 tatcttacat ctactaacgt caatatgctt catccgtttg cggaatctat ttctaaaaaa
     3961 ttcaatgtag atgtgagaga ctaccaattt gatatttctg attatagcat ccatgctcag
     4021 ttcttaaagt cgcttccaga aattccagac gttgtggtct gttgtgctgg atattataaa
     4081 gatcaggaaa aagccatcag cgattttgga gaaacatata gaacgttaag cgttaattat
     4141 gtcggtgtag tttcgattct aaatttaatt tcaatcgaat ttcaaaaaag aaaatcaggt
     4201 tccattatcg ttctaagttc agttgcgggc attcggggaa ggcaaatgaa ttttctctat
     4261 ggaagtgcaa aggctggaat tactacttat ctttctggac ttagaaatcg tttatttaaa
     4321 gacaatgtgt ctgtaacaac gattctttta ggtcctgttt atacaaagat gtccgaaggg
     4381 cataacctga tgccagtcct aacggcaaag cccgaagtag ccgcagaaaa aattgtaaaa
     4441 gcagggcttt ccaaaaaagg agaggtttat attctctggt attggaagtg ggttatgttc
     4501 attatccaat tgattccaga gtttatattc aaaagattga agccgtttta gtttgattta
     4561 gagctataac taacgtcagt ttggagtaac aaagaattta taagattacc accgaggaca
     4621 ccgataactg aacgatacag aaacgattga taattttgaa ttgtcgtttc tttatctgtg
     4681 tccatcagtg gtaaaaaagt tttatccttc tgttgttcca actccgatat ctgtttgttt
     4741 tccttctctg cttggatgga taaaaattcc aaatccttta ggtcttgaag attttccatc
     4801 ttttgcaaaa ttctttgcag tttgttttaa gtcttcggtt gattctctat gatcgcgttt
     4861 aatcggtgtt tgccccgcaa gtgtttctac atgtagagtt tgtgttggga aagcaaagga
     4921 aatatttaac tctttcgcaa ggttaataat ctcaagatag acattttgac gcaatatcat
     4981 atcttgtccc cattctgtaa cggttgcaaa ataattgagt agaatatcta gacttgaatt
     5041 tccaaatcct ttgaaaatta catgaattct gtcttggact acgagttcat ttttgcgtag
     5101 aatgtttttg attccttcta agaatgcttc aattttttca ggaggtgtgt cgtaggtgag
     5161 tccaagagta gtgttcgttc ttctataacg tctaagaccc atgttgtcaa ttttagaatt
     5221 tgccacttct gagtttggaa tggatactac agaatcataa aaggttcgaa ttcgagtgca
     5281 acgaaatccg atttcctcca catccccttc gatccctcct aggataatcg tatctccaat
     5341 tttaaaaggt ctatctagaa atatcatgat agaaccaaat agattggctg ctgtatcttt
     5401 tgcggcaaga gctaaggcaa gacctcccac accaagacca gctaaaactg acataacgtt
     5461 tacacctaga ttttgcattg caaaaagaga accgaggata acaacgagta ttcgcactgt
     5521 tctagaagca agtggaatga tttgagcatc tattaaaaat tcacttttat tttgctttgc
     5581 aatcatttcc acatattcag taaatgcttt ggttagatca taaaagaggc gaacaagatt
     5641 aaagctgagt aggatttgta aaatgatatt acttatttta aatggagtgc cgtctatatc
     5701 taaaaaatcg aggcatatat accagaatcc tacaatcacg atataagcta aaggtttatc
     5761 acagaaggtt aagatttttt ctttccagtt gattttacct tctggaactc gattgactat
     5821 atgggtgatt aaatgagata tgcttctggt tatttttcca actagaaaac ctaaaaggat
     5881 tgagaggata ataccaatta tctgccaaac atagagagag aaaaatttaa atttcatcca
     5941 acctggtaga ctttcagacc aaggatcttt gtaacctgct ccataaccag aattagctaa
     6001 ataaggaaga tgttttgttt tggtataaaa ttcaaaagca cgtgcaactg tatctctaga
     6061 aaataaaaat tcatttgctc tatctcctcc ttctacctta gaaatagaaa tctctgtatt
     6121 tccaaagatc cattttgtga gaggatcatt gggattttct gatttatcag gaatcgttag
     6181 tggatctatt acatcgattc tatctattac ttcttttaat agaatagcgg actctctacc
     6241 tttctctgcc ctaagaccaa aaggaattcc gtctaggttg agacaacgga cagccgtatc
     6301 caattcagcg atgagttgtg aatttttaga ctctaaacct tttttgtaat cattcataga
     6361 ttttaaaaag actgcaaagg tttctctagg tgtttcaaat ttattaggtt ctaaaatatt
     6421 ttctctagtt ctatcttctg caaataaagg aattaaagcg attaatccaa atagcaatat
     6481 ctgtttcata tcctacaagt ttatttctat tttaagggag tctataagaa ttttaatgcg
     6541 aaagaaatgg tatttgaaaa gcttgccgat agggatacca atgtcattga gataagaaaa
     6601 atatttgaag attaccaccg agaacaccga caaccgaacg ataaaaaacg atgttatctg
     6661 tgaataatca aatcttttat ctgtgttcat ctgtggtaat catttgtatc gaaacagaaa
     6721 gagaggaatt tttatgaagt attggttgtt taaatcagag ccaaatgtat tttccataga
     6781 ggatttaaag aatgcaaaaa attctacctc tagttgggag gggattcgaa actttcaggc
     6841 tagaaatttc ttgagagatg aagcaaaaaa aggagattta gttttatttt atcatagcag
     6901 tgttgagccg atggctgtgg tgggtgttgc agaggttgta aaagaaggat atatcgatca
     6961 ttttgctttc gataaaaaaa gtgattatta cgatccaaag tcaaatccgg ataaaccgca
     7021 gtggtatatg gtggatgtaa aattcaaaaa agaatttaaa agacctgtta ctttaaaaga
     7081 aattaaaact tacaaggaac tttctaaaat gcgtcttgtg caaaggggag ctagactttc
     7141 gatccaaccg attacaaaag aagagtttga ttttattgtg aggcttgggg gctaatatct
     7201 attagtcact gtttctaatt tataaaatct gtttgatgta aaaaaagaaa agattaccac
     7261 tgacttgcat tgagttctct cgaatgtgta aacaccgatc tcatttaatt taagaaaatt
     7321 aaaatttgca gttttagagg cataagcgaa gggatttgta tatcttgtta ctgaaaaatt
     7381 tttaaatcga aaaattgcag taggagagct tggagtagaa attctttttt ctctagattt
     7441 ctatgagaaa taggaattgc cgagccagtc taactttaaa acttggttaa tgtaaaaatt
     7501 caatctctca tttgtataat aagtgggagt atttaatcca aatgaaaatg gtgacgaaat
     7561 gaatttaaaa tcgatagcta tctttccgag aggaataatt ctatttatac tcctttctct
     7621 catacttcaa tgctccacaa aggatgtaaa aaaaacaaac aatcgaagaa tgattggaat
     7681 cgatgccaat ctttccctga ctgaaaatgt ttttcaaaag gcaaagacag tcaatgaaga
     7741 aatatttgca agacaggatg tgtctgacgt tcgcacagta acggttaggg atttacttga
     7801 taaacttcaa ttgaaaaaag atgaatactg cgacaataaa ataaaggatt tagatgattt
     7861 ccccgtagat ccagctaata tgtgttgtga tttaaatttt atgaacatag atcctagaaa
     7921 gggtaaagtt cattatggat ttgatatccc actcgccatt ggaactcctc tcaaatgtcc
     7981 agacaaagaa gattgtttgg ttgtgcgcgt ctatccggag tggatcacat cggatggaat
     8041 gccaaaacaa actatctatg gggaatccct tcttgtatat ttcaaaaagg caggctattt
     8101 tggtttttat gctcacttaa aaaagatcta tggaagaccc ggtcgtattt ttaattataa
     8161 agaggtaatg gctctttctg gaaattcagg cagtctttct acgggagctc atttgcattt
     8221 taccttgtat cgaaatcagg actgttctaa tggagtcttt gatgagaaaa tgagacttgc
     8281 cgttttagat acaatcgaag aaatcaaaat ctttgaagcc aaagaaaaag gactagatcc
     8341 ctcttcttat aacattgaag agaagctcgc ggatgacgta ttgaaaagat gtaaagtgaa
     8401 gatgagtcaa gaggcgaacc gagcttgtat tgttcgaaac ttcccgagag attttattga
     8461 tcctctagaa gtaacgtccg atcatacaat ccaaagactg gttttagaaa ttaatccaaa
     8521 gagaatgaga gatcgagatg tattgcgttt attttcctat caccaaagag ttcgaaacaa
     8581 ttcaggaatt catagtgaaa tttcaaaggt atccaataca actccaacga atgtagaaaa
     8641 tttaaaagac ttactcagaa atgtaaatat cctaggcgaa gagatgtagg tagatgtttt
     8701 caccacggat gaacaggcga aggcgaaggt cgttggagcg aaggggggaa taaacacaga
     8761 tgggtctatc tgtgtttcat tttatgttgt gtagggtaga ctattctggg gatttcaaag
     8821 ttatccgttc gagatgtatt taaaatgaag cctatattaa ctaacgctag ttcgatgtaa
     8881 caaaaaattt aaaagattac acattatgtt gcgaccctta ggaagcaaca ttgagtttga
     8941 acactgatgg acacggataa aataataata attattaaaa ccatccgcgt ttatctgtgt
     9001 tcatccgtgt tcatccgtgg taaaaaaatc tttacttcga atttagtttc aattaaaata
     9061 agggtccttc gaattcaatc gttgcagagt cgatttgttc attcccgatt acttctattc
     9121 caactggatt tttatttaaa tgaaatacaa ctttgaaaaa tgtatcgtta tacgaccgag
     9181 acatttcaat cagagtgatc gaattttcat ccgaaagaag gattggaaaa tcaaaactag
     9241 aaatgagata ttgaatcgtt tgtcttaaag gtaggttgat tttttttagt ggcttcatac
     9301 tgaatttata aagcttatca tctacacctt ctttcttttc tgtatagatt ccgctatttt
     9361 cttcaaataa ataagatttt tcttccatga aaaattcata gggaatgtat ttgtaatgaa
     9421 ggattttaat ttctggataa ggaaattctt tcaaaggaag aatgatataa aataaatacc
     9481 caactgcgat cgcaaaaaga ggaatttgta ttttgactgg ttttatcagg atgaggcaaa
     9541 gagagattag cccgatcatt ggattttgtg ttttaagcag agagtaagaa tagattttat
     9601 tttcggaaaa agtaaattcc tcaaaaccaa aaagtaaaaa gaaaatatag ggagcaatga
     9661 gtagacttgc gagggacgca gctaagctga aactctttgc taaatagaga agtagactaa
     9721 cggctaataa aaaaaacaga attgtatagg cattgaagta gatacttttt aattcaaaga
     9781 atctagtaga aaacagagta gaaatataaa agatagaata gaatagaaat ccgaaggcaa
     9841 taaacaaagg gagtcttttt aatttctcta caagcttttt gtcctgaaag gaaaaaaaat
     9901 tccagaatag gcatgtaaag actcctagat taaaagagaa tgaaatcgct tcgaatatca
     9961 tagtggtgaa atttgtgtaa tgaccatatt attttagctg taaaaaaagc taactacaat
    10021 tatcttgtat ttcatggact tgatcgaagt aaaaatttca gacatcacac tgaccaacgt
    10081 tggattcgct gttttcttga aaccaaagga agatttgttt gattctaaag tagttccgat
    10141 atttatcggt ccacttgaga cacatgcgat tacaagtgta ttagatggga tcacaccacc
    10201 tagacctatg acgcatgatt taattatgat gatcatccca gtcttaggtg ctcagattat
    10261 taaagtcaca atcgatgaaa ttattgataa tactttttat gctaaaatat cactccgaaa
    10321 agatgaggag atatttttaa tagatgcaag accatctgat tccatagcca ttgccctaag
    10381 agcagaggcg ccgattttta ttactaagag tgttttagat gaggcgggnn nnnnnnnnnn
    10441 nnntatgaga ggaggggaaa ttccatctga gcctagtatt gaagaggcgc tttcctctga
    10501 gccaaatcct cctaaaactc agttgcaatt gttagaagat tctctggctg cagcgatcaa
    10561 aagtgaagac tatgagactg cggcgcgtat tcgagatcaa attaagaaga tcatagaaag
    10621 ctcttaaccc cagaactggt tggtaatcca agccattttg tgctatgcaa ggggattgca
    10681 ttttataatg tggtattcga actaaaaaat ttggagaaaa caaaatggaa aaagttctaa
    10741 cagatgtagt aaatgcagga atcgctttat ttaaaactgg agaagaagtt ctaaaaaatt
    10801 ctttagttga tttcgaaaag aaattcaacg aaatcaagca aaaaggtgaa cttgatcaat
    10861 ctgagcaagc agttcaagta agagatttat taaacaaaac tatcaaagac acaaaagatg
    10921 tactagataa tgcaaatgca agctaccaag atatcgttag caaagctcaa acaaactttg
    10981 aaggaatcta caatcaagta gatgcagcac ttccagagca agttaaaaca caagctaaag
    11041 ctgctctcga tgagttaaaa gctgttatcg aaaaatacaa acctgcagca aaataatcgg
    11101 ttagtagtaa tgatttaatg ccggtatcat ttttagccgg catttttctt tttatccccc
    11161 aaaaaacttt ttaaatgact tataaacggg aaaaactggt cttattttta acagttttta
    11221 ttgatatgat gggtttttcc gtcatcttcc ctatatttcc agaaacactt cgtttctttc
    11281 tttcgagaga aaaagatccg attctcaact ttttcattca tattttaaat ctatttaata
    11341 caaactcgga ccatagattg tttattgttc tatttggtgg gtttgtagga agtatttact
    11401 ccatcttaca gtttttcttt gcgcctattt ggggaaggtt atctgacaaa gtaggtagaa
    11461 agccagcctt ggttttgacc tcaacgggaa attttctagg gtatgtggtt tggttctttt
    11521 cgggaagctt tactctgttc gtgatttcta ggctcatcac aggctgtatg ggtggaaata
    11581 tttctgttgc atcggcagct atggcggatg tgaccagcga gaaagaccga gccaaaggaa
    11641 tgggaatgat tggagctggg attggtcttg gctttatgtt tggtcctccg ctgggaggaa
    11701 ttcttgcctc tgtggatttg actcaaatct ttcctgcctt gaccgagtat ggagtgactg
    11761 tattttcctc ttccgctctt ttatctgttt taattgcgct cgtgaattta ctcttggttc
    11821 tatttgtttt tagagaaacc tttcagccta ccaaagaacc tacgaaaaga gaattgcatc
    11881 ctatcttagg attaaggact gcaactgtgc aagagcttcc tcttctctgt cttatctatt
    11941 taattttctc tctctccttt tctgggtttg agttttgtat caatttcttt ctttatgatc
    12001 tattccaatt taacccaaga gaaattggtt atacctttgt ctatttggga agtatcatca
    12061 ttttgattca aggtggagtg attcgccgca tttcaggaaa agtagcagag aaaaaaattg
    12121 cgatgacagg tgctgttaca ttgctctttg gacttactgc attgtctttt tccaaatcag
    12181 tagggcttgc tattttttct cttggcttta tttcaatggg aagtgccttt ctaaatccag
    12241 gtctttcctc tctcgcttct cttttttctc ccccgcaaga ccaaggtaga aatttaggaa
    12301 tcatgcgagg cttcggtgct cttgctcgtg gcgtttctcc tgttactttt tctcttcttt
    12361 attttacctt cggtgcttat tctacttttg cagtctctgc tgttttaatc ttaattgtga
    12421 ttggcatgat acgttatgtg ccagagaaga gtaagtagtt ttttaaaacc tatctatcac
    12481 ctaaagtcaa tctgaggcgc tatgcgattg ataaatatga tttagctatt cgcgtgaatc
    12541 tggattctac tttcacaaaa aaagagagtc tttaaaaatt ataacatctg atggtaaaat
    12601 cacactctat acaatgaaag agttgaaaga actgatacca aacgtcaaaa gaaaagtcgc
    12661 attctaaaaa taaaaaagtg ggtttggtct aaacacttct tagtgttccc gaaagtcagt
    12721 atgttcggta taccaaatgc tagtggatca aaattctttc gggaaattac ttttgtcatt
    12781 tggtatataa aaaacaaaaa gattgaatgg gcagatcaat caaagaaact aaacatcata
    12841 aaaaatattt ggaatttcat aaagggttaa gaaaacaatg gatgaaaaaa taaaacaaga
    12901 agtagaagac ggtgttcgtt gcccagattg tttagaagta agagcaattc aattttatga
    12961 cacagaacca accgtcagca gattattgca gaatcataat aaaggcttag tttcctttta
    13021 ttttgctaaa attcattctg actggtcgaa tcttcattaa tacttataaa ttaattcgct
    13081 ataaattttt cgggggagaa aagtttccgt gaaactaaaa aaattcatct tctcctaagc
    13141 gaaagggatt aaagggagag cttggacatt gtgaaataaa aaacgtagag acgcatggca
    13201 atgcttctta acagaagata acaccgattt gcccttataa aaaattttaa attctcgact
    13261 ctatgctaga gagattttta atattagaat ggaaataaaa aatgccaatc agtgatgatg
    13321 atttaaaagc atggcaggaa aaaatagaat gggaaacgga aaccaagacc atttcggatt
    13381 atttctttgt gacccaatgc caaacctgca agcattttaa ccaatggagt agaaaaaagc
    13441 tacggtgcac tgcttatcaa gacttgattc ctttgaaaat tttctatggt aagcacgatc
    13501 accattttcc ctacccagga gatgaaggaa ttttatatga gccgatggaa acggataata
    13561 caaattagcc tttaaatatt cagaattgaa caaagcgata gagacagatt aatagaggaa
    13621 tggtattgca aacctaatat tatgaactat agtgtttata tctacataat agacatttta
    13681 atacttgcac attgctttcg cattcagaga atgttctacg gattaaagga accaattgaa
    13741 atatataaat attttatctc tcataataaa ttgtaatctt aaatttctaa tatttgttcc
    13801 aatcatcaca atcaaaatct tagcggatga ttttattttg aaagcagagt atgcaaataa
    13861 agaatatcga tgtagaatcg aagagtctgc acacaaaaca aaaaagtcct ttccttttca
    13921 taaattccga ctagggtgca cattttttca agaagagatg ataggttatc agtctggatc
    13981 tttaatgggc gttattgtac ataaaaataa aggagagtat ttgaaatgga tggagcgcgg
    14041 aaatcaaagt ggttttttgc caatgactaa taattgtttc ataagcgtta gtcattggaa
    14101 ggcgattaat ccactagatt ttaaaaaata taaattaagt ccagaaaagt atgaaaaata
    14161 tcgtataaat tttttagatg tgtttgaaat gattcgtaag gatattgcta atactgagtt
    14221 ttgtagatag gatgcatatt agaaaaatat tattggtatc cctctcctaa attcaatcgg
    14281 aggatttatt cagcaaataa cgcaaatttg cctttacaaa aacatattca tatattctct
    14341 gaaaacaaaa actttatttc gtttgtatcc tgtgctttct ataagtattt cttccttcat
    14401 aaaaactttg actaattcat ttgcagcttt ttgagaaagc ccagtgatgg actgaacatc
    14461 tttacttgtc actagtggtt ttttaaataa accatggagt aatgcatgag ccgtgggtgc
    14521 acgttttccg atctttatta ttttattttt ttcaatcatt gctttcagat caatgatctt
    14581 ctttagggtt atggcggact tctcagcagt ttcaatgact cctactaaaa agaactttat
    14641 ccattgcgtt aaatcgtttt tagtccgcac aaaggttagg ttatcataat aaagagtttt
    14701 atttctttcg aaaaaatctg ataaataaag tagcggttta tcaagaattc cgctcgagac
    14761 aagataaagt gtaattaaaa gtcttccaat tcttccattc ccatctagaa agggatgtat
    14821 ggtttcaaac tggtaatgcc cgatcgctat acggatgaga tgtggaatct ttaaatcagt
    14881 attattcaag aataattcca aatcagaa
//
LOCUS       DAOQOS010000129        14791 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_2066_length_14791_cov_5.614346, whole genome shotgun sequence.
ACCESSION   DAOQOS010000129 DAOQOS010000000
VERSION     DAOQOS010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 14791)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14791)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14791
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2066_length_14791_cov_5.614346"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(169..3666)
                     /locus_tag="PLF66_21075"
     CDS             complement(169..3666)
                     /locus_tag="PLF66_21075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66597.1"
                     /translation="MKIPLTALESNVEPLPSINLSFDGIDPVRRLQDFYLSKKGSLVI
                     NTVFQSIYSQEKRKRIAAITAPPGSGKSYLALLIGSLLYNDKSWNKEILSILENQNKT
                     YRDEVEKEIEQYSKSNTRLIPIYIVGESDNLRRTLIENLLRGLEQFAARNDFYELAFD
                     FFKEDLDLQRKSGDVKRLISKVGDTYCNPDDFFTGYKMILDFCQSLGFNGFVIFNDEF
                     NRLLAKDQALSSKDLDFLQDFAEYNLRLKSTIVLHYLLLHKGISQYLNGISVDRRKEW
                     LKIEGRFYQIHFQEELADVYGLIANYLKRNTNYKISNDKDSLILSQIKTAWKINPFLG
                     EEIGPELKEIAHRAYPLHISNLVALPLLCNLLGQNERTLFGYLSDVPSRKKKDSMYLD
                     DLFDYFDTSIDKLGLDDSLLTRWHYGRNALSEVTQKEKTRVIKALTILSVINRPNLLP
                     ASIRWISFSLGLEDDTVKSILKELSGENLALYREANKTYSIYYGSSIDLARKIDELLP
                     TISIGTIARTIEDIFSLSPVYANSYNAEYFTSRFYSRKFILEENIREFIQYKADEESE
                     PLIKNKIENTKHHLSIHLTEILEKEEKSGASGVILYFLGNPNSDIAQALRTLVGEKDK
                     KNKILSVFSEIAFHEIPLLKKYAACVKLSEDLELIRRDPRIKNDTKIYLSDLHDKLHF
                     TLRSLFEKSKAKLVGSYNFDESESLSQIVSGILLKRFPHCPKVNSEFINRENISPIIR
                     NTRKKILRDMIAGDLSLGSKEKGYGPDVAIFRSLFSIKSVYKTTDGRVQYSFVSPEDY
                     LGNHDEGLRKVFEAIHKYLQEESERTTLDRLYEILTSEPFGMYSEMIPFYFLAALLEK
                     SYSFSLYEEDRYEKEINSDILEKLHANPKNFKIHIIRSNALLEKYLMKISELFASNDA
                     TRLVQAGEARLERKINENKIYRSVLAILYWYTKLPECTRHTKDLSNENSKFLQIISHS
                     VNPELLLLEEIPELFGSNLESIREKDLEQFIAKLKKAKQEIESHYNQLIEKIAASTLK
                     IIKPYITSQKSQHSLSEAISSFKMDNEYKLELLRKHDSSFQKLMERIRSNYESEFALL
                     ESLTSLLCDVHPRYWKDNTIQEYEFKLTTELAKIHVATLMSNPGESAHMKMDRILQEY
                     RSLKKEERQMLINRLKEEV"
     gene            complement(4107..4439)
                     /locus_tag="PLF66_21080"
     CDS             complement(4107..4439)
                     /locus_tag="PLF66_21080"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HMZ66598.1"
                     /translation="MKEPAYKIEYLEAEAIRRILSTFQPEKIILFGSYIYGNPSYDSD
                     LDILIEFKNIPNKGKLIIDIRKQFKDFPVAKDILVIAQDEIDAYKEKKWTIYSNALEK
                     GKVVYEKK"
     gene            complement(4492..5322)
                     /locus_tag="PLF66_21085"
     CDS             complement(4492..5322)
                     /locus_tag="PLF66_21085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase family
                     protein"
                     /protein_id="HMZ66599.1"
                     /translation="MPKERHIVSLSGGKDSTALAIFMQKNYPTLDVEYVFCDTGEELP
                     ETYSYLEKIETFLGKKIVRLRASRTWDQFLEDFSGYLPSARSRWCTANLKIKPFEEYV
                     GEDLIYSYIGIRSDESREGYISSKTNITPKYPFKEHGIDKEGVMRILEESGLGLPEYY
                     KWRTRSGCYFCFFQRRKEWLGLKENHPALFEKAKQYETSNKDGSAQNFTWIQGGTLDQ
                     ILKDEEGIKRRHDQYESRVKRDRQRKSKRLIDVFSSELSFEDALNMEDDAEGCLVCHV
                     "
     gene            complement(5376..6512)
                     /locus_tag="PLF66_21090"
     CDS             complement(5376..6512)
                     /locus_tag="PLF66_21090"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HMZ66600.1"
                     /translation="MSSIHSLQSKKKTDVNYSVTFAEHSDTEFLWIKFFGDVFPTRID
                     FEKEFSPKTKPLLMKYLDGKKIHSKYGNLSENWELYKHIPSYRIQENGLSQLGKQIYY
                     ISLKYNFLLEVTITYTNTVMLELYFVEKTKVIVDVIRFLKEFNRITQKEKDYISLHLL
                     SMREHVPSLAAIEKRLPLRQINIENHYNDSFEIVHAKILKELAKKNHKGLILLHGDYG
                     TGKSTYIEYLIQKFRKKKFILVPSYIAEKIDSPDLISVFTRYPNSVFIIEEAEKLVRS
                     REQDSSIAGLLNSSSGLLGNLLNSIFILTFNTNESTIDPALLRKGRLILSYKFEKLTV
                     EKAKQLLSLLGKDANIDSPMSVAEIYNLEENQFSKPDTRRIGFE"
     gene            complement(6710..7489)
                     /locus_tag="PLF66_21095"
     CDS             complement(6710..7489)
                     /locus_tag="PLF66_21095"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HMZ66601.1"
                     /translation="MSGKNSYLYVWSGFALFYSPSLVNTSHTHYAASLLHSFGNPIEL
                     EGANGDKLHMQSVFLPSNYHHKLKSDSPLLILQIDPDSPEYAPLRKLSETKPTDISGK
                     LESILEREMQTPLNSSNGITLIRKILSYVGENDLEQKTPHSKIDYRIKNVIRYLKSLE
                     DLPPKIPLSLLAKEASLSSDRFRHLFAEEMGLSIRRYLLWLRIRKAGSLLQKGFSLTD
                     SAHHAGFTDSAHFSKTFKQNFGITPSLVLASANVKVKYFEE"
     gene            7600..8115
                     /locus_tag="PLF66_21100"
     CDS             7600..8115
                     /locus_tag="PLF66_21100"
                     /inference="COORDINATES: protein motif:HMM:NF017897.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF962 domain-containing protein"
                     /protein_id="HMZ66602.1"
                     /translation="MKTLEERMSGYAAYHKDPRNKLTHFFGVPMVYYSPLIAFGWVPL
                     FEISGFTVTLAWVLFAITMIWYFTLDVQLATIMTIISLPIVYATDVAAKLPFMESLTI
                     FLAIKIFGWVIQLTGHVFEGRRPALVDNLVQALMAPLFLIAETLFFFGIRKDLEKNVQ
                     ERVALLGNSKF"
     gene            complement(8181..8954)
                     /locus_tag="PLF66_21105"
     CDS             complement(8181..8954)
                     /locus_tag="PLF66_21105"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HMZ66603.1"
                     /translation="MQIPDWFLSILRCPETSKPLRYENEIFMRGDGISYKTIDGIPSL
                     VFPKDLAGLDKDWNLFYNLFAPLYDWNERFFGKLLTGVDVVEGRKEIISLLGLQKGMR
                     VLEVSPGPGVFQELIREKIGSEGEFVSVDLSLGMLKQCQKKQSKVNTILLQANGSYLP
                     FAENSFDALFHFGGINLFTESDKAIQEFIRVVKKDGIVSWGDEGFSPKLPDNFKNRIL
                     VKTNPGFLKPHPVIPDSIASVQVSEVYGGYAYLVVGKKR"
     gene            complement(9030..9254)
                     /locus_tag="PLF66_21110"
     CDS             complement(9030..9254)
                     /locus_tag="PLF66_21110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66604.1"
                     /translation="MEKIYQDKIMSFSPIERFYMAFDMLDTAKALALASLPKDLSKEE
                     VRVQMFLRFYGNDFSEIEKEKIISHLRNIK"
     gene            complement(9268..9807)
                     /locus_tag="PLF66_21115"
     CDS             complement(9268..9807)
                     /locus_tag="PLF66_21115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66605.1"
                     /translation="MIEQIQVLENILKYLKDQKIDYILTGSLAMTFYAEPRMTRDIDI
                     VLSIENLSPKSFTQFFQSDFYIDIDSIQMAIHYRKMFNILHNATGLKIDFIIQKENEF
                     EKQKFKNKKMIKIKETDCFIISKEDLIIQKVLWSRESFSEMQFRDVRNLTRLDYNKLY
                     VEEWIKKLNLEETWNKIYV"
     gene            complement(9815..11176)
                     /locus_tag="PLF66_21120"
     CDS             complement(9815..11176)
                     /locus_tag="PLF66_21120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66606.1"
                     /translation="MRTFIIQTEAKDPETLKSFVEFEVEEGGRSSEPKEDKGQRVDYP
                     KLTVYINDIPFEDITRSYNTIRSETTELKWEVKPSRDFDSKDVCVLIKSESGKILDRF
                     PYKIPASMNEPGQQPLVLDIHPKGGTIGDTITMKVQNPGKDLDKIVVYILKPKQEQEG
                     YVYLDKEKCALFPFYLSEEKITNKTSYHEIKFTLSNYLANRCGFDTEKYNLFEKMFGK
                     DISIKLSINARPSKIEKLTILNESWKGFAVTMGALITLLFLGILAYFSKKINFFPDIL
                     LDPETNSFSLANFQSFLWTLVLMGSYFYIAVCQGLILGNPELPEFNFSLIGLLGVSYG
                     GMIAANYVDKRKKHMVIKKDKPELRDLIFDPSGGIDMARMQLFGFNLICLLLYIYYLL
                     KANPLNGLPSIPETLHTLLGTSQAGYIGSQAVTQMITPNPDAEKEKTEEEKPVEEVKK
                     KKK"
     gene            11435..11716
                     /locus_tag="PLF66_21125"
     CDS             11435..11716
                     /locus_tag="PLF66_21125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66607.1"
                     /translation="MKTILLLTFLFGFPLLAEEDLCTTFKTQRKCESKDGIKNKCKWL
                     KAKELCQSEQSIVESTGNNNEKGIDATEDESDRPSRKGKNKNKNKIKTP"
     gene            complement(11739..12689)
                     /locus_tag="PLF66_21130"
     CDS             complement(11739..12689)
                     /locus_tag="PLF66_21130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008590795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HMZ66608.1"
                     /translation="MNGKNMQRPNELKQNKSKQFYLIYVIGLFTLTSCASTLVNTFVR
                     YERNKASLTKKEIQVENHKIVYLEGGEGETILLIHGFGADKEHWTRMSRYLTDKYRVI
                     SPDLPPFGESTKLDSEDYGVLPQAKRVHLFTQALGLKKYHIVGNSMGGHIAGVYTSEY
                     PDEVLSLALFDAAGVTSPNPSKLSEKLKEGKNPLIVSNEEEFDGLMKFSFVNPPYIPG
                     FFKREVMKKAAANKPYSEKVFSKLMQDGNVLEKRLSKIKAKTLILWGDSDNIIDVSSV
                     QVFEKGIVNHKTVILKECGHAPMIELPEESAKQYLNFIQN"
     gene            complement(13031..13795)
                     /locus_tag="PLF66_21135"
     CDS             complement(13031..13795)
                     /locus_tag="PLF66_21135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66609.1"
                     /translation="MVLRLLIIILLFLTHCLFAEEEGKEDLPSYHKGKLLLEIGFGNL
                     NSKISSKSDPNTLTELYFLYDVLPYLNSTDSQNVNLGIFKYGSYQKPVAENYNARFGI
                     EYALLDWFGVGISGSGTRFSIKNITPGEYLTYPSYFILPSSRYTSSPTYTDYINLYRQ
                     DFHVNFGTIDVEASLHYPLGSFDPYIRYGYGIAPGYSGVYKSSHVAGFRFFWDESYIQ
                     IEYLQNFLYGIGFHIQQTDYLLERGFRFGLGYSFFN"
     gene            complement(13806..14342)
                     /gene="def"
                     /locus_tag="PLF66_21140"
     CDS             complement(13806..14342)
                     /gene="def"
                     /locus_tag="PLF66_21140"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012388559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HMZ66610.1"
                     /translation="MAARKVLKLGDPILRKISEPVTEDETKTKDFKKLIKDMFDTMKF
                     ENGVGLAAPQIGILKRLVVVGVENSERYPGTPDIEEKILLNPVITPLGPQGAGFWEGC
                     LSVPGMRGLVERPSKIKMEWYDEKWNHHSEIIEGYKAIVFQHECDHLDGILYVDRLKD
                     TKLFGFTDVIDTQGKELD"
     gene            complement(14466..14538)
                     /locus_tag="PLF66_21145"
     tRNA            complement(14466..14538)
                     /locus_tag="PLF66_21145"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14503..14505),aa:Ala,seq:ggc)
     gene            14608..>14791
                     /locus_tag="PLF66_21150"
     CDS             14608..>14791
                     /locus_tag="PLF66_21150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015682429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66611.1"
                     /translation="MDTIESLQRKVAAQSDIIKGYEKVLKLNEKELSNADQIIKMYES
                     IIDYSSSEMKNAQQTLQ"
BASE COUNT         4698 a         2682 c         2398 g         5013 t
ORIGIN      
        1 taaatctgca attgtatgtg aacttggttt ccaatttatt ctatacataa aattctccta
       61 tttatctagt atgaatgcaa tattatcaaa tattagaagt tctcaatctt tcagaaacaa
      121 cccatagtta tatgaaagta tttcacttta ttttaataac tagattaatc acacttcctc
      181 tttcaaccgg ttaatgagca tttgccgctc ttcttttttg agagagcgat attcttgtaa
      241 gatacgatcc attttcatgt gagcactttc tccaggatta gacatgagag tcgcaacatg
      301 aatttttgca agctcggttg taagtttaaa ttcatattcc tgaatcgtat tatctttcca
      361 ataacgtgga tgaacgtcac ataaaagaga agtaagcgat tcaagaagag caaattcgga
      421 ttcataatta gaacgaattc tctccattaa cttttgaaag ctagaatcat gcttacgaag
      481 tagttctaat ttgtattcat tatccatttt aaaggaagaa attgcttctg agagagaatg
      541 ctgtgatttt tgagacgtta tatacggctt tattatcttt agcgtgcttg ctgctatttt
      601 ctctatcaat tgattgtagt gactttcaat ttcttgtttt gcctttttta attttgcaat
      661 aaattgttct aaatcttttt ctcgaatact ttctagattg gatccaaata attcaggaat
      721 ttcttctagc aaaagaagtt ctggattcac tgaatgagag ataatctgta gaaatttaga
      781 attctcattt gataaatctt ttgtatgtcg tgtgcattcc ggaagttttg tataccaata
      841 caaaatagca agcacagatc tatagatttt attttcattg atctttcttt ctagtcttgc
      901 ttctcctgcc tgcactaaac gtgttgcatc attcgaagca aataattctg aaatcttcat
      961 tagatatttt tctaataatg cattcgatcg gataatgtga attttaaaat ttttaggatt
     1021 tgcatgtagc ttttctaaaa tatcagaatt gatttctttc tcataacgat cctcttcata
     1081 aagggaaaag gaatacgatt tttctaataa agccgcaaga aaataaaaag gaatcatctc
     1141 cgaatacata ccgaatggct cagaggttaa aatttcgtac aatctatcaa gagttgttcg
     1201 ttccgactct tcctgcagat acttgtgaat cgcctcgaat acttttctaa gtccttcatc
     1261 atgatttcct aaataatctt ccggagaaac gaaactatac tgaactctac cgtctgtcgt
     1321 tttataaact gattttatgc taaaaagact tctaaaaata gctacatcag gaccatagcc
     1381 tttttctttt gatccaagac ttaagtcacc tgctatcata tctcttaaaa ttttctttct
     1441 tgtatttcga atgattggag atatattctc ccgattaata aattctgaat ttactttcgg
     1501 acaatgtgga aagcgtttta gtaatattcc agatactatc tgagaaagag attctgattc
     1561 atcaaaatta taggaaccaa ctaacttagc tttgctcttt tcaaaaagag atcgaagtgt
     1621 aaaatgaagt ttatcatgta gatctgatag gtaaatctta gtatcatttt ttatccgagg
     1681 gtctcttcta attagttcta aatcttcaga aagttttaca caagcagcgt atttttttaa
     1741 aagaggaatt tcgtgaaagg caatttcaga aaagacagat agaattttgt ttttcttatc
     1801 cttttcgcct accaaagttc gtagtgcttg tgctatatca gaatttggat ttccaagaaa
     1861 atagagtatc acgccactcg cgcctgattt ttcttctttt tctaaaatct ctgttaaatg
     1921 gatagataaa tgatgttttg tattttctat tttatttttt attaatggtt ccgactcttc
     1981 atctgcttta tactgtataa actcgcgaat attttcttct aaaataaatt tacgagaata
     2041 aaacctagat gtaaaatatt ctgcattgta tgaatttgca tagactggac ttaaactgaa
     2101 aatatcttca atagttcgtg caatggttcc aatagaaata gtcggtaata actcgtctat
     2161 cttgcgcgct agatcgattg agctaccata gtagatagaa tacgtcttgt tagcctctcg
     2221 atagagagct agattttctc cgctcaactc tttcaaaata gatttaacag tatcatcttc
     2281 taaaccgaga gaaaaagaaa tccaacgaat agaagctggc agtagattcg ggcgattaat
     2341 aacagaaaga atagtaagtg ccttaatcac tcttgttttt tctttttgtg ttacttccga
     2401 gagtgcattt cttccataat gccaccgtgt cagcaaagaa tcatctaacc caagtttatc
     2461 aatcgaagta tcaaaataat caaagagatc atctaaatac atagaatctt ttttcttgcg
     2521 ggatggaacg tcagacaaat atccaaataa agttctttca ttctgtccga gtaaattaca
     2581 aagaagtgga agtgctacta aattagaaat atgaagtgga taggctctgt gtgcaatctc
     2641 ttttaattcg ggtccaattt cttctcctag aaatggatta atcttccatg cagtttttat
     2701 ctgagataaa attagagaat ctttatcatt tgagatttta taatttgtat ttctcttcag
     2761 atagtttgca atcagtccat atacgtcagc taattcttct tgaaaatgta tttggtagaa
     2821 gcgaccttct atctttaacc attcctttct acgatctaca gaaataccgt ttagatattg
     2881 ggaaattcct ttatggagta acagataatg taaaacaata gtcgatttaa gtcttaaatt
     2941 atattctgca aaatcttgta agaaatctaa atccttagaa gataaagctt ggtcttttgc
     3001 aagcagtcga ttaaattcat cgttaaatat tacaaatccg ttgaatccta acgattgaca
     3061 aaaatccaaa atcattttat agccagtaaa aaaatcgtcc ggattacaat acgtatcccc
     3121 tactttggat atgagtcttt tcacatcacc agattttctt tgtaaatcta agtcttcttt
     3181 aaaaaagtca aacgccaatt cataaaaatc attgcgagcg gcaaattgct ctaagcctct
     3241 caataaattt tcaattaggg ttcttcttaa attgtcagat tctccaacta tatagattgg
     3301 aatgagtcgt gtatttgatt tagaatattg ctcaatttct ttttcaactt catcgcgata
     3361 ggttttattt tgattttcta atattgatag aatttcttta ttccaagatt tatcattata
     3421 taaaagagat ccgatcaaaa gtgccaaata ggattttcca gatccgggtg gtgcagtaat
     3481 tgccgcgatg cgttttcttt tttcttgcga atagatagat tgaaagactg tatttataac
     3541 aagagagcct ttctttgata aataaaaatc ctgtagtctt cgaacaggat caattccatc
     3601 aaaacttaaa ttgatagatg gtagtggctc gacatttgat tctagtgctg ttaatggtat
     3661 tttcatgaaa tttatttaaa gtattctgca cagatttata agagttacat agtttgtcaa
     3721 ctctcttgaa aaaacttaaa atgtttgtcc ccaaaaaact attcaactta tcaaaatcta
     3781 aaaccaaatc actttgataa gggaaagaac aaattacaca catataacct ccccatcacc
     3841 catcgaatcc tttatcggtg taaatcggtg gtaatctttt tctcccgcaa agagaatagc
     3901 aagccttgag aaagaaatga tagaacacaa acaaagttat ttgagtagtg attctgatag
     3961 agggaggtat attgaaaaag cggtttgagt aaaggaaggg ctataccaag actatacgaa
     4021 ccactatacc aagtagctag agagtgtata gaaactgtga agttgggagg aagaagtcaa
     4081 catattgaag ccatactttc cattcctcat ttcttttcat atactacctt acctttttcc
     4141 aatgcattgc tataaatagt ccatttcttt tctttgtatg catcaatttc gtcttgagca
     4201 atgacgagaa tgtccttcgc tacaggaaaa tctttgaatt gtttacgtat atcgattatt
     4261 agctttcctt tgttaggaat atttttgaat tcaatcaaaa tgtctaaatc actatcatag
     4321 gagggatttc catatatgta agatccaaaa agaataattt tctcaggctg aaatgttgaa
     4381 agaattctac gaattgcttc ggcttctaaa tactcgattt tataagctgg ctctttcatt
     4441 tttcctcttt ctgttttgaa agcaaagaat tttttctatc actacaaaaa cttacacatg
     4501 acaaaccaaa caaccttctg cgtcatcttc catgttgaga gcatcttcaa aagaaagttc
     4561 agagctaaat acatcaatga ggcgtttgct tttgcgctgg cgatcgcgct taacgcgtga
     4621 ttcatactgg tcatgacggc gcttgattcc ttcttcatct ttcagaatct ggtcgagtgt
     4681 gccaccttgg atccaagtga agttttgcgc agatccgtcc ttattagagg tttcatattg
     4741 tttggctttt tcaaaaaggg caggatggtt ttcttttaaa ccaagccatt ccttacgacg
     4801 ttggaaaaaa caaaaataac aaccagagcg tgtgcgccat ttataatatt caggaagtcc
     4861 gagtccactt tcttctagaa tgcgcataac gccttctttg tcgatgccat gttctttgaa
     4921 tggatactta ggtgtaatgt ttgttttact cgaaatataa ccttctctcg attcatccga
     4981 gcgaattcca atataactat aaattagatc ctctcctaca tattcctcaa aaggtttgat
     5041 ttttagattg gcagtgcacc aacgacttcg agcagaagga agatagccag aaaaatcttc
     5101 tagaaattga tcccaagtcc gggaagctct caagcgaaca atttttttac ctagaaaggt
     5161 ttcgatcttt tcgagatagc tataagtctc aggcaactct tcgcctgtat cacaaaaaac
     5221 atattccaca tccagtgtag gatagttttt ctgcataaaa atggcaagtg ctgtggagtc
     5281 ttttccaccg gaaagagaaa caatatgtcg ttctttaggc ataagtctag tttgaaaata
     5341 agacataagt ttgcaagaaa aattcagagc atcttctact caaagccaat tcgtcttgta
     5401 tcgggttttg aaaattggtt ttcttctaag ttgtaaatct cggcgacact cataggagaa
     5461 tcgatgtttg catcttttcc taaaagagaa agtagctgct ttgctttttc tacagtgagc
     5521 ttttcaaatt tataagagag aatcaatctt ccttttcgaa gaagtgcggg atcgatagtc
     5581 gactcattgg tattaaatgt aagaataaaa atagaattga gtagatttcc aagaagaccg
     5641 ctagaagaat ttaatagccc cgcaatacta gaatcttgtt ctctcgatct gactaacttc
     5701 tctgcttctt cgatgataaa aacagaattg ggataacgag taaaaacaga aattaaatca
     5761 ggagaatcaa ttttttcagc gatatacgac ggaactaaaa tgaatttctt ttttctaaat
     5821 ttctgaatca agtattcaat ataagtgctc tttcctgttc cataatctcc atggagtaaa
     5881 attaaccctt tgtgattttt ctttgcgagt tcttttaaga ttttagcatg cacaatttca
     5941 aaactatcat tgtaatgatt ttcaatgttg atttgtctca gtggtaatct cttctcaatg
     6001 gcggcaagag aaggaacatg ctctctcata gaaagcaaat gaagagaaat ataatctttt
     6061 tctttttgtg taattcgatt gaattctttt agaaatcgta taacgtccac aatcaccttt
     6121 gtcttctcga caaagtaaag ctctaacatc actgtatttg tataggtgat cgttacttcc
     6181 aataagaaat tgtatttcaa agaaatataa tagatttgct ttccgagttg ggatagtcca
     6241 ttctcttgaa tgcgataaga gggaatgtgt ttatataact cccaattttc ggaaagattt
     6301 ccatacttag aatggatttt tttaccatct aaatacttca tcagaagtgg ctttgtcttt
     6361 ggtgaaaatt ctttttcaaa gtcaatccgt gttggaaata catctccaaa aaacttgatc
     6421 cataaaaatt cagtatccga atgctctgcg aatgtaacag aataatttac atctgttttc
     6481 tttttagatt gtaatgaatg aattgaactc ataattgcgt gatttccata ttttccattt
     6541 cgaatttact gtcgaaagat ttttattgaa tgcatacata gaaacgccta aagaggatag
     6601 aaagctaata tcattcatca accacgaaga gcacgaaagt caggaagaag taagaagaga
     6661 ttacaaaact caccttcgcg atctttttta taaaaaatct taagactctc tattcctcaa
     6721 aatattttac ttttacattt gcactcgcaa gaacgaggga gggagtgatt ccgaagtttt
     6781 gtttaaatgt tttactaaaa tgagcagagt cggtaaatcc tgcatgatga gcagaatcag
     6841 ttaaactgaa tcctttttgt aaaaggcttc ctgcttttcg aatcctaagc caaagcaaat
     6901 aacgtctgat cgaaagtccc atttcttctg caaatagatg ccgaaaccga tcagacgaca
     6961 aagaagcctc ttttgcaaga agagacaaag gaatctttgg agggagatct tcgagtgatt
     7021 ttagataccg aatcacattt ttaattcgat agtcaatctt agaatgtgga gtcttttgtt
     7081 ctaaatcatt ttctccgaca taagacagaa tttttcgaat gagtgtaatc ccattcgaag
     7141 aattaagggg cgtttgcatt tctctttcta agatcgactc taatttccct gaaatatcag
     7201 ttggtttggt ctcagaaagt tttcgaaggg gcgcatattc cggcgaatca ggatcgattt
     7261 gtaaaatcaa tagaggggaa tctgatttta acttgtgatg gtaattggat ggaagaaata
     7321 cagactgcat atgcagttta tcaccgttag ccccttctaa ctcgattgga tttccaaaac
     7381 tatggagcag agatgccgcg taatgagtat gggaagtatt tacaagtgag ggagagtaaa
     7441 agagagcaaa gccactccaa acatataaat aagaattttt cccactcatc ttttctttcc
     7501 cgttttaact ttttttctct ttttagccgt tttttacaag cctgtttttt agaaatatgc
     7561 tattctagat tcatacaatc aatcaaaaag gatataaata tgaaaacact agaagaaaga
     7621 atgtctggct acgctgccta ccacaaagat ccaagaaaca aactcactca tttttttgga
     7681 gtgccaatgg tttactactc tcccctcatc gcatttggat gggttcctct ctttgagatt
     7741 tctgggttca cagtgactct ggcttgggtt ctatttgcca ttacaatgat ttggtatttt
     7801 actttagatg ttcaacttgc tactatcatg acgattattt ctttgcctat cgtatatgca
     7861 acggatgttg ccgcaaaact tccttttatg gaatctctta cgatttttct tgcgataaaa
     7921 atttttggtt gggtcattca gcttaccggt catgtctttg aaggaagaag acctgctctt
     7981 gtagacaatc tagtccaagc ccttatggct cctctcttct taattgcaga aactcttttc
     8041 ttttttggaa tccgcaaaga cctagaaaaa aatgtccaag aaagagttgc tctcctcgga
     8101 aattctaaat tctaaaaaac gttagtatct ataaaaaaga tttgtgtaga taaacattta
     8161 cacaaatcct tattagccct ttatcttttc tttcctacca ccaaataggc atagcctcca
     8221 taaacttcag agacttgaac tgatgcaata gaatctggta taacaggatg cggttttaaa
     8281 aaacctggat tcgttttgac tagtattcta tttttaaaat tgtctggaag ttttggcgaa
     8341 aaaccttcat ctccccaaga gacaattcca tcttttttga caacacgaat gaattcttga
     8401 attgctttat cgctttcagt aaaaagatta attccaccga aatgaaacaa ggcatcaaag
     8461 gaattttctg caaatggcag ataactccca ttagcctgaa gtagaattgt attcacttta
     8521 ctttgttttt tctggcactg ctttaacatc ccaagagata aatcgacaga cacaaactct
     8581 ccctcagaac caatcttttc tcggatcagt tcttgaaata cccccggtcc cggagatact
     8641 tccaaaactc gcattccttt ttgtaagccc agaagagata taatttcttt tctaccttct
     8701 actacatcta ctccagtcag tagttttcca aagaatcttt cattccaatc atacagagga
     8761 gcaaatagat tataaaaaag attccaatct ttatcgagtc ccgctaaatc tttgggaaaa
     8821 accagtgatg gaatcccgtc tatcgttttg tatgaaatcc catctccacg catgaaaatt
     8881 tcgttctcat aacgaagtgg cttagaagtt tcagggcatc taagtataga aagaaaccaa
     8941 tctggaattt gcataataaa ttcatttatt ccaaagatta cagtttagtg tcaaatggtt
     9001 tttactcagt taacaataat ttgccgcttt tattttatat tcctaagatg agaaattatt
     9061 ttttcttttt caatttctga aaagtcattt ccatagaagc gtaaaaacat ttgaacacga
     9121 acttcttctt ttgataaatc ttttggaaga gatgcgagag ccaatgcttt tgctgtatct
     9181 aacatatcaa atgccatata gaatctttct attggagaga aggacatgat tttatcttga
     9241 taaatctttt ccatttctgg acttgtatca tacatagatt ttattccaag tttcttctaa
     9301 atttaatttt ttaatccatt cttcgacata tagtttatta taatctaatc ttgttaagtt
     9361 tcgaacatct cggaattgca tttcggaaaa actttctctt gaccataata ctttctgaat
     9421 gataagatct tctttagaaa taataaaaca atctgtttct tttattttta tcattttttt
     9481 atttttaaat ttttgtttct caaattcatt ttctttctga attataaaat caatttttaa
     9541 acctgtagcg ttatgcaaaa tattaaacat ttttctataa tgaattgcca tttgaatgga
     9601 atcaatatca atataaaaat cactctgaaa gaattgggta aagcttttgg gagatagatt
     9661 ttctatagat aaaacaatat caatatctct tgtcattcta ggctctgcat aaaatgtcat
     9721 agctaaagaa ccggtaagaa tatagtcgat tttttgatcc tttaaatact ttaaaatatt
     9781 ttctaagact tgaatttgtt ctatcatagt ttgcttattt tttctttttc ttcacttctt
     9841 ctacgggctt ctcttcctct gttttttctt tctctgcatc cggatttgga gtaatcattt
     9901 gtgtaactgc ttgcgaacca atatatcctg cttggctcgt gccaaggagt gtatgcagtg
     9961 tctctggaat agaaggaaga ccatttaatg gatttgcttt gagtagataa tagatataga
    10021 gaagtaggca aattaaatta aacccgaaaa gctgcattct agccatatca attccaccac
    10081 ttggatcaaa aatcaaatcc cttaattctg gtttgtcttt tttgattacc atatgcttct
    10141 ttcttttatc aacataattt gccgcaatca ttccaccata gctcacaccg agtaaaccta
    10201 tgagtgaaaa attaaactca ggaagttcgg ggttaccaag gattagcccc tgacagactg
    10261 caatgtaaaa atagcttccc atcaatacta acgtccacag aaaagattgg aagttagcta
    10321 gagaaaagga atttgtttct ggatcgagca aaatgtctgg aaagaaattt atcttcttac
    10381 taaaataagc aagaattcct agaaagagta aagtgatcaa agctcccata gtaaccgcaa
    10441 aacctttcca ggattcattt aagatagtta gcttttcaat tttacttggg cgtgcgttaa
    10501 tagaaagctt aatactgata tcttttccaa acatcttttc aaatagattg tatttttctg
    10561 tatcaaatcc acaacgattc gcaagataat tagaaagtgt aaatttaatt tcatgataac
    10621 tagtcttgtt tgtaatcttt tcttctgaga gatagaaagg aaataaagca catttttctt
    10681 tgtctaaata aacatatccc tcttgttctt gttttggctt tagaatgtaa actacaatct
    10741 tgtctaaatc ttttcctgga ttttgcacct tcatagtgat tgtatcgccg attgttccgc
    10801 ccttgggatg aatatctaat acgagtggtt gctgtcctgg ttcattcata gaggcaggaa
    10861 ttttatatgg aaatcgatct agtatttttc cagattcact cttaatcagg acacaaacat
    10921 ccttggaatc aaaatctcta gaaggcttca cttcccattt caattccgtt gtttcacttc
    10981 taattgtatt gtaacttcgg gtaatgtctt caaagggaat atcattgata tagactgtga
    11041 gttttggata atctaccctt tgccctttgt cctcttttgg ttcactgctt cgaccgcctt
    11101 cctctacttc aaattccaca aaggatttca gagtttcagg atcttttgcc tcagtctgga
    11161 tgataaaagt tctcatggta aaatctttga taaagaaatc tctaatttca atcggagtat
    11221 tatttaccct ttgacaattc tctttggcaa ggagaggcaa atggagtgta aacgaagaaa
    11281 gaaaaagaat tatctttaaa aaagttgaga actggaaagg caggcgaaat acattgagtt
    11341 tagaaaaaca ttctatatat aggatattca tactcaaata agattgcaga acaatggaac
    11401 tgagtcaaga atgttatttc caaggagatt ttttatgaaa acaattttac tactaacctt
    11461 ccttttcggt tttcctcttt tggcagagga agatctttgc acaaccttca aaacacaaag
    11521 aaaatgtgaa tccaaagatg gaatcaaaaa taaatgcaaa tggctcaagg caaaagaact
    11581 ctgtcaaagt gaacagtcta tagtagaatc tacaggcaac aacaatgaga aaggcatcga
    11641 tgcaaccgaa gatgaatccg accgcccttc tcgcaaagga aaaaataaaa acaaaaataa
    11701 aattaaaacc ccataatcag gctaatagag agtatcctct aattctgaat aaaatttaaa
    11761 tattgcttcg ctgattcttc tggaagttca atcatcggag catgtccaca ttctttcaat
    11821 ataactgttt tatgatttac aattcctttt tcaaatactt ggacagagga tacgtcaata
    11881 atattatctg aatcgcccca aagaattaag gttttggctt tgatttttga aagtcttttt
    11941 tctaatacat tgccatcctg catgagttta gaaaatacct tttcgctata tggtttatta
    12001 gccgcagcct ttttcattac ttctctttta aaaaagcccg gtatataagg tggatttaca
    12061 aaggaaaatt tcatgagtcc atcaaattct tcttcattcg atacgatgag tggatttttt
    12121 ccttctttta gtttttcgga cagtttgctt ggattcggac ttgtaacgcc tgccgcatca
    12181 aaaagagcaa ggctcaaaac ttcatccgga tactcagatg tataaacccc ggcaatatgt
    12241 cctcccattg aatttcctac aatatgatat ttttttagtc caagtgcctg ggtaaaaaga
    12301 tgaaccctct tcgcctgggg caaaacacca taatcctcag aatctaactt tgtgctttct
    12361 ccaaaaggag gaaggtctgg ggaaataact ctatacttgt ccgttaggta tctagacatc
    12421 cttgtccaat gctctttatc ggctccaaat ccatggatga gtaaaatggt ttccccttct
    12481 ccaccttcta gatatactat cttatgattt tccacttgta tttctttttt tgtgagactt
    12541 gctttgtttc tctcatagcg aacaaaggta tttaccaatg tgctcgcgca agacgtgagt
    12601 gtaaagagtc cgattacgta aatcaaatag aattgtttgc tcttattttg ttttaattca
    12661 tttggtcttt gcatattttt cccattcatt ttattttctt tttaatttta tttataactc
    12721 tcattaggca aggtgaaatt catctaacgt gaattcggta taaggaattg acaaattctt
    12781 cagaccgtca ttcccgaccc tatggagatg ctggatttgc gacctagcaa atgggcaaaa
    12841 tcgccgcaac cgccaagcaa tatcgggcaa tgtcattcag ttaaggatct ttgtgtcatt
    12901 ccataaagct gtcactccct aaatcctcta tcgggagtat gcagaacgat aatttcgaga
    12961 gacccctgat aatcgccccg acggcaactg gatgcgaatc gtaggaagaa ttgaatttca
    13021 atttttcaaa tcagttaaaa aaagaatatc ctaaaccaaa tcgaaaccct ctttctaaaa
    13081 gataatcagt ctgttgtata tgaaatccaa ttccataaag aaaattttgc aaatattcaa
    13141 tctgaatata agactcgtcc caaaaaaaac gaaatcctgc cacatgagaa gatttataga
    13201 ctccagaata tccaggggca attccatatc catatcgaat ataaggatca aaacttccga
    13261 gtggataatg aagactagcc tctacatcta tcgttccaaa gttcacatgg aaatcttgtc
    13321 tatataaatt tatataatca gtataagtag gtgaacttgt atacctcgaa ctaggtaaaa
    13381 taaaataact tggatacgtt aggtattctc caggcgtaat attctttata gaaaaacgtg
    13441 ttccggatcc agaaatgccg actccaaacc aatctaaaag ggcatactca attccaaatc
    13501 ttgcattata attctctgct actggttttt gataacttcc atatttaaaa atacctagat
    13561 ttacattctg tgaatcagta gagttcagat agggaagcac atcgtataaa aaatacaatt
    13621 cagtcagagt atttgggtct gacttagagg atattttgga attcaaattt ccaaaaccaa
    13681 tttcaagtaa aagtttgcct ttgtgataag aaggtaagtc ttcctttcct tcctcctctg
    13741 caaaaagaca atgagtcaaa aagaggagga taataataag taaacgaaga accaaagaat
    13801 atttcttaat ctagctcttt gccctgtgta tcaattacgt ctgtaaagcc aaaaagtttt
    13861 gtgtccttca aacgatctac atataaaatc ccatcgagat gatcgcattc atgctgaaaa
    13921 acgatcgctt tataaccttc tatgatttca gaatgatgat tccatttttc atcataccat
    13981 tccattttga ttttacttgg tctctctact aaccctctca ttccagggac ggatagacaa
    14041 ccttcccaaa atcctgcacc ttgcggacca agaggagtaa tgacagggtt taagagaatc
    14101 ttctcttcta tatcaggtgt tcctggatag cgttctgagt tttctactcc taccactacc
    14161 aatctcttta aaataccaat ttgtggtgct gctaatccca caccattttc aaacttcatt
    14221 gtatcaaaca tgtctttgat gagttttttg aaatcttttg tttttgtctc atcttcggtt
    14281 actggttcac tgatttttcg taggattgga tcacctaatt ttagaacttt tctagcagcc
    14341 atatcatttc ctcaaatgtt tgattattac cattttctat ttataaatga aaaattcaag
    14401 caagcaaaaa aaacattgac atcctattag gaagcattag aacctaggaa gatgtaattt
    14461 cggattggag acgaggggga tcgaaccccc gacctcttgc atgccatgca aatgctctcc
    14521 cagctgagct acatccccgt aggaaaatga tagtcaaaaa tacttcctat gtgtcaattt
    14581 aactttttaa tttttaaagg tgattttatg gatacaatcg aaagtctaca aagaaaagtt
    14641 gctgcacaaa gtgatataat caaaggttac gaaaaggttc tcaaactaaa tgagaaagaa
    14701 ctctccaatg ccgatcaaat cataaaaatg tatgaatcta tcatagacta ctcttcttct
    14761 gagatgaaaa acgcacaaca gacattacaa g
//
LOCUS       DAOQOS010000130        14593 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_2110_length_14593_cov_7.605654, whole genome shotgun sequence.
ACCESSION   DAOQOS010000130 DAOQOS010000000
VERSION     DAOQOS010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 14593)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14593)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14593
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2110_length_14593_cov_7.605654"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(311..772)
                     /locus_tag="PLF66_21155"
     CDS             complement(311..772)
                     /locus_tag="PLF66_21155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66612.1"
                     /translation="MEKKIISIFIIILFFIICSGKSNREKEKYQFVEEKNSNTSSQNF
                     PKENVQGMVPEFKINDLKMNGDKVEFLLEVRGVIDHLVLKEISFVEGTEVDGDVIETY
                     GTIQNSSILIKTDKPEFFPNRKLYWRHASGLKYSLIIKKDITKEDIREEGF"
     gene            963..1247
                     /locus_tag="PLF66_21160"
     CDS             963..1247
                     /locus_tag="PLF66_21160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66613.1"
                     /translation="MKPFLIFILISTFSIYAQSSGIGTPGTGQGTPSGSFGTPGSGIG
                     NPGSGVGTPGTGQGRPSGSFGTPGSGVGNPSPGQGSPYGNSSSFGTGTGK"
     gene            1347..2006
                     /locus_tag="PLF66_21165"
     CDS             1347..2006
                     /locus_tag="PLF66_21165"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HMZ66614.1"
                     /translation="MKKDEIKLIVSRIVITFLFLVSANCLTTGIYKASKEGDLATVKR
                     LIEEGASVDESDSWSLHPIHYAAEKGNIEIAKLLLEKGASIHALDVREYTPLHFAAKE
                     GHAEMVQFLLTKGANKELKSSVTAYERTPLDLAVMGNHEETKQVLYNWIIIEEIPKNI
                     KTQKIPTGIYKDIVTLRNGDVLENVKAVIQMSTVLVTNSKGKSTEYKKAEIKKISVRK
                     I"
     gene            2448..2996
                     /gene="folE"
                     /locus_tag="PLF66_21170"
     CDS             2448..2996
                     /gene="folE"
                     /locus_tag="PLF66_21170"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004459199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HMZ66615.1"
                     /translation="MEDAVKKILTDIGEDPTREGLLDTPKRVKKAYEFLTSGYTTDIT
                     KLVNGAIFEEHVEGMVIVRDIELYSLCEHHLLPFFGRAHVAYIPNKKIIGISKIPRIV
                     DVFARRLQVQERLTDQVANAIQEILDPLGVGVIMKAKHMCMMMRGVEKQNSELLTSCM
                     LGEFRDSSITRNEFLDLIRTGQ"
     gene            2998..3420
                     /gene="hemJ"
                     /locus_tag="PLF66_21175"
     CDS             2998..3420
                     /gene="hemJ"
                     /locus_tag="PLF66_21175"
                     /inference="COORDINATES: protein motif:HMM:NF015608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase HemJ"
                     /protein_id="HMZ66616.1"
                     /translation="MYLWFKALHIISLIAWFAGIFYIWRLFVYHTETNSEDVKATLAI
                     MERKLYTIIMRPAMFATLFFGFGLFYLQWNAFSNSIWIWLKIFLVILVVGQHHMANFY
                     RKKLLEGVVYRSKKFRILNEIPTLLMILIVILVVFKPF"
     gene            3561..4481
                     /locus_tag="PLF66_21180"
     CDS             3561..4481
                     /locus_tag="PLF66_21180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010573296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMZ66617.1"
                     /translation="MIKVQNLTKFYSDKPAINGLNFQIKDGEIVGLLGLNGSGKTTTI
                     RILSGFLIPNDGEVWVDDLNLFTDPLEIKKKIGYLPETPPLYEDYTVSGYLEFVARLK
                     GVSDIPTELTRVCQKTNLEEVKDNYISHLSLGYRKRVGIAQAILGSPKIVIMDEPISG
                     LDPKQIVEIRYLIKSLSKDHTVIISSHILSEVYLVCDRFLFLHQGKLVYDYSREQLEK
                     EMSKISVLHVGLRSSSRLACQAFLENIDTSAEVKLLEGDNDYYLFYIKPKDIHIYRDQ
                     MLLGLHTKDFKLELLKKEDLTLEQFFIDSI"
     gene            4503..5228
                     /locus_tag="PLF66_21185"
     CDS             4503..5228
                     /locus_tag="PLF66_21185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002102852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMZ66618.1"
                     /translation="MQNVKWIFLKECKLFFNTYMAALVLGGTAVLNALLVLISNFHGT
                     ENYEDAALITFFSFMSTIIISMMILAMGSIVEEKNKGTMELLYTAPISDLEIVLGKFL
                     FGAFVCLIITIFINGLFPILLYSYWKAPIPIFVSGSVGVFLLGCFIYSVGLFGSSLGK
                     NQMISLLIAVLIVMILWVVGFFSHLFQAKTRSILFHLHIFSHFISFTKGVLPLSGIVF
                     FSSGIFTFLYLTVKSLESRRWVG"
     gene            5230..7086
                     /locus_tag="PLF66_21190"
     CDS             5230..7086
                     /locus_tag="PLF66_21190"
                     /inference="COORDINATES: protein motif:HMM:NF021351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gldg family protein"
                     /protein_id="HMZ66619.1"
                     /translation="MNYNIFFPILSLLSLFIYFLTIDFFTTSLSKLSWNLLGLLLISS
                     DLIYRIIQSRSQDSDSQKNIRLFVSSLGLTSFLIYQVRDYLDFPIPPGGSEQASPFPK
                     IRDFLLILIILTSIAFLLYTILLEISRQSANAQHNLRQKKNTILQNTLYSFLWISPLL
                     VAVNYFAVQKNYNFDLSSSGKFSFSTTSRQILKDVKKDIKITAFFPRPLESDERTKRE
                     SFAIGLIRPEVEIILDQLKSLNSQISVQFINADVEKELMSDFTQVSNGVILIRSLKSN
                     TDVGSNPYMEERVVVQTKKDLEDIERKLVQAVINVATSQKKFYFTTGNGERYGSAFQS
                     LPSEQITRLTSMLSFYNYGIKELGFNEGWPEKIPDDADTLFIIGPTTALSEVARNTIF
                     SYIEKKGKLFITIDPAGNEDFAWLLEKAGLSIKKENIRQVEGRPQIIANKFQPHPIEE
                     LIYNKDLGVFFLANAYFELSPSGNSIFISNTLLESGFTAFADLNKNGKLDKEEKQNSF
                     ILGTILVPKEDPANPAKTENPSRIIIYSGTNWITNGFIVDRLNSILAVNSVNWLNQSP
                     LTEKILPKKEEEPVVSISDSQKIVIWSVGLFIYPAIVILSLGFYTISKKRKK"
     gene            7083..8132
                     /locus_tag="PLF66_21195"
     CDS             7083..8132
                     /locus_tag="PLF66_21195"
                     /inference="COORDINATES: protein motif:HMM:NF025598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HMZ66620.1"
                     /translation="MKKILMVAIAFIVLAFVFFYSEERHEEGLTEIAYWKIEPDEIRY
                     YPPKDHSEFSNFIKSELTFKRIKTGLKSSPYFTVEGIDPESKKPFLYEAGYNVKNLFT
                     EMSVLNVKSINPANPDLLKKFQISEQSSPALEIYLSSKPQKKIYLGEQSNDKNYRYIL
                     SDKEVLTTIGHVFQKFSQSPFDLRDRQYLHFGEFELARMIFQSEGVRITIENLPETKN
                     GTQMSRWFKTTSGKNKMDPSQGSQLFSLLQAFKADLYPDEIEGEGFLVAKELTATETF
                     ANLQLILTNGMHYKIKLFPKVILKNKSYYPVMKEVDKFQIESTSYTNETLINQMLDLL
                     KKIQTDPEWKDPKGK"
     gene            8134..8568
                     /locus_tag="PLF66_21200"
     CDS             8134..8568
                     /locus_tag="PLF66_21200"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HMZ66621.1"
                     /translation="MLELTKGDTPLVFPKFPNEDKMTESEICCKCTGCCRYVSVDIKK
                     PRSKDTIDLYVWYLLHKNVQIYIDNDKGWNLLFITTCTKLQSDGKCGIYPTRPKLCKD
                     YSPSGCSRTGKDHTHLFMTPDALLNYLEEEKAKKGKKKQLKR"
     gene            8581..8871
                     /locus_tag="PLF66_21205"
     CDS             8581..8871
                     /locus_tag="PLF66_21205"
                     /inference="COORDINATES: protein motif:HMM:NF022179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="late competence development ComFB family
                     protein"
                     /protein_id="HMZ66622.1"
                     /translation="MGQSNIVHIEKWTSKVSNSVEEDIRSELKNIIQENPAWGWEQIS
                     IQDVYAYALNQLPPIYSEKGKKPEYLLSIEEIRNAIYTAIRKIEENPIHYTK"
     gene            8929..9684
                     /locus_tag="PLF66_21210"
     CDS             8929..9684
                     /locus_tag="PLF66_21210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012476140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66623.1"
                     /translation="MHKLFISALLITFISCNTNISLKSKNENKAKLSFPEITSILSAT
                     DSVYYFYTRDIDKTLTTNCGVATPGTVGGTTGATGTPGATGTTGTTGTTGTSTSRRII
                     LSQLVFKTGETLTMKFQYDSNQFQGSLDQQQGFTLTGGIFGHTVKGTQGTVKWSGQGV
                     GYIDETNNAAQTLTYMIVDLNLRGTFTANSSSSGTTPVECNTTDGINCTSSVSTTKCF
                     TQDYIKCVANNTGGTAITISGSINCNAPNVIPQ"
     gene            9692..10843
                     /locus_tag="PLF66_21215"
     CDS             9692..10843
                     /locus_tag="PLF66_21215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016758971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HMZ66624.1"
                     /translation="MIDFSLVIPTFNEAENICILIPRVISLLSAANISFEIIVVDDDS
                     KDLTWKAVQESFANEPRVRVLRRVNKKGLSSAVLDGMAIAKGKSFGVMDADLQHDENI
                     IPKMLQELEKHDIVVGSRVVGDGGYGEWGLIRKLMSKGATLLAKIMLPIPVGDPMSGF
                     FVLKRELYEELVPKINPRGFKILLEFLARKKGIKAKEVGFVFKTRIHGETKMSGSVIQ
                     NYLVALYDIRFGHLISITFLKYGFTGLTGVFVNLFGQFFAMTIFHLGSSQAYYENFTK
                     PFLAVAFGFELSVLSNYIINNLWTFSDSKINGLKGNLLGFFKFNIVSIVGFIIQISVW
                     RFSYQVIQDEFPQFLFNGLTYICNFAGIVLATAGNYFLNKNFTWQEKDR"
     gene            10847..11344
                     /locus_tag="PLF66_21220"
     CDS             10847..11344
                     /locus_tag="PLF66_21220"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HMZ66625.1"
                     /translation="MNEITVLLDQHKYIRSRLIQFIKEVAATPYAEEALHWSMPFGKG
                     RAHIAWQIMHCAATYDRYLHFRILNAEPKNPDLVKAYGNGSVPNPKLIVKVEEILSAL
                     ELTTKPYYNYFESLPIDKLNEKPHESADRTYKEILHLLNWHEASHHGQAQITWNAFRA
                     FRGHI"
     gene            11744..12298
                     /locus_tag="PLF66_21225"
     CDS             11744..12298
                     /locus_tag="PLF66_21225"
                     /inference="COORDINATES: protein motif:HMM:NF019235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1564 family protein"
                     /protein_id="HMZ66626.1"
                     /translation="MENESLHDIQEGSNIISSYKISFPRNDYIYSEFSSTILIPDQYF
                     KYFQKKIYTHKGVRSYISYLLSKYQIYITNGLVPSYSNVTTKYQDKGQNLHKIAFRPK
                     PEDWAELKLYRLTFGMSISAFLVYLLIADSTDFAEAFSDYLETVGISRVPNFNLSAKV
                     FLLNDRTNYTIVFQYRKSRIRQNG"
     gene            complement(12469..13866)
                     /gene="purF"
                     /locus_tag="PLF66_21230"
     CDS             complement(12469..13866)
                     /gene="purF"
                     /locus_tag="PLF66_21230"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017213797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HMZ66627.1"
                     /translation="MYNNSEAANFTYLGLYSMQHRGQESSGIVSTDGEHLYRYAGMGL
                     VANLFSQEKLKELKGHAAIGHNRYSTTGDSFLRNAQPVRVESHLGGIALAHNGNLVNS
                     YDIRRKMEEEGSIFQTTIDSEVIVHLMAKSKSKVLLDALGDALRQVEGAYSLLILTSK
                     MLIAVRDPNGFRPLVIGQKNDGTIVFASETCAFDITDTQYVRDVAPGEMVVVDSFGMK
                     SYFPFSPRKSSLCIFEFIYFSRPDSYIFGESVYKVRKKLGEYLSRELPVEADVVMPVP
                     DSANVAALGYSEATGIPYRTGLIRSHYVGRTFIEPDQKIRDFGAKIKYNVIREVVNGK
                     RVVIIDDSIMRGTTSRKIVKMIRNAGAKEIHMRISAPPTVSPCYYGIDIPTHSELIAS
                     SHSIEEIRKYLRVDSIAYLSMESMHKAVEQDYKNSNYCDACFSGKYPIHFDTSRHSNQ
                     KTLFQEFSMEKTANY"
     gene            13997..>14593
                     /locus_tag="PLF66_21235"
     CDS             13997..>14593
                     /locus_tag="PLF66_21235"
                     /EC_number="3.1.-.-"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015677023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0033890 - ribonuclease D activity
                     [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HMZ66628.1"
                     /translation="MQINSKYILVDNKKSLELAVVNLANSRLIAIDTESSGYYTYVSK
                     VCLIQISANGKNFIFDPMAQIDLKALDPIFRNPSILKLFHSAVDDIKALKRDFGFQFK
                     NIADTMYSSKLLGLEHNSLNYLVEFYHKIFLSKTEQKSNWEKRPLDRHQLQYAALDTA
                     YLESIWNLMKDELEKRNLLQEAISEFERMADEPYVAKET"
BASE COUNT         4999 a         2482 c         2427 g         4685 t
ORIGIN      
        1 ccctttgttt ccttccttta attttttatt tcatcaccct gataggttac gaattttcta
       61 atattcccaa actcatcttt tgtgcctcaa agtattcatt ttgcctagac tgagttagga
      121 aataagattc tatttctact atcgtatcga tagggtatag atattttcaa tttctaatat
      181 atacaaatga ttgtaattag tagaaaaaat tttacgaatc ttgctataaa gaaaaggaaa
      241 acaaggaagc gtattctctt tttttcttaa aacaaataga taaatagaat catgaatttg
      301 agctaaatcc ctaaaaacct tcctctctta tatcctcttt cgtaatatct ttcttgataa
      361 taagtgaata ctttaatccg cttgcatgtc tccaatagag ttttcgattt ggaaaaaatt
      421 ctggcttgtc tgttttgatt aagatggagc tattttgaat cgttccgtat gtttcaatta
      481 catccccatc aacctctgtc ccttctacaa atgaaatctc tttcaaaacc agatgatcaa
      541 tcactcctct tacttctaat aaaaactcaa ccttatcccc attcattttt aaatcattaa
      601 ttttaaattc cggcaccata ccctgaacat tttcttttgg aaagttttga gaagaagtgt
      661 tagaattttt ttcttcaacg aattgatatt tctctttctc tctattactc ttacccgagc
      721 aaatgataaa aaagagaatg attataaata tagaaataat ttttttttcc atttgattga
      781 tctaaatcct caatttcaaa atttaatttg tttcctctca tcagaataca gcgcggatat
      841 tttccgaact aatttataga aacaactgag atctaaattt tttctagaaa gcaattgaat
      901 cgtttctaaa aaatgaaaca ttcccaaata aattcttata tattacaata gtctaataga
      961 acatgaaacc atttcttatt tttatactta tttctacatt ttctatctac gcacaaagtt
     1021 ctggtattgg aacaccaggc accggacaag gaactccgag cggtagtttc ggaacaccgg
     1081 gttctgggat tggaaatcct ggttctggag ttggaacacc cggaacagga caaggtagac
     1141 caagtggtag ctttggaaca ccgggttctg gggttggcaa cccaagccca ggtcagggca
     1201 gtccttatgg gaattcatcc tcctttggaa caggaacagg caaataacta ttatccgcca
     1261 agatgctatt ttttttagta aaaactcttt gcatagtgtg gtatatgaat ttttatatac
     1321 tattgtaacc ttataaggga agacaaatga agaaagatga aattaaatta attgtatcta
     1381 gaattgtaat tacttttcta ttcttggttt ctgccaactg cctcactact ggaatttata
     1441 aagcttctaa agagggagat ttagcaaccg taaaaagact cattgaagaa ggggctagtg
     1501 tggatgaatc cgatagttgg agtctccatc caattcacta cgcagccgaa aaaggaaata
     1561 ttgaaattgc aaaattactc ttagaaaagg gagcttcgat tcatgcatta gatgttagag
     1621 aatacactcc attgcatttc gctgcaaaag aaggtcatgc agaaatggtt cagtttttat
     1681 taacaaaagg tgcaaacaag gagttaaaat cttctgttac tgcttatgaa agaacgccac
     1741 ttgatcttgc agtcatgggt aaccatgaag agacaaaaca agttttatat aattggatta
     1801 tcatagaaga gattcctaaa aatattaaaa cacagaaaat accaactgga atttataagg
     1861 atatagtaac tctgcgaaat ggagatgttc ttgaaaatgt caaagcagtg attcaaatgt
     1921 caactgttct ggtcacaaat tcgaaaggaa aatccacaga atacaaaaaa gcagaaataa
     1981 aaaaaatatc tgtaagaaaa atatagataa cgcatcggcg tttttcttat tcttgaaatc
     2041 ctccttgatt cccccaagta tgcattttac atttcatcag gcagatattc caggattctg
     2101 gaatccattg atactaacgg ttaatctatt gtgaaataca tcccgggttc aaccaaatcc
     2161 attttgaaaa caggatggaa gatactttag gacctttctt gaaaacagag aaagcatttc
     2221 agcaaggcat gcttgcctgt atttacaaca atcagcagtg aaggtttaaa aatccaattc
     2281 aagaaagtgt tagatttttt agtaattgat tatccctcgc tatcaagaaa acccaaagtg
     2341 aataatacta tgttgcctcc gagtttaatt ctaaaaataa acctctttac aatgattcta
     2401 attttaaaga aatgtcaatg aggtagaaat taaagaagga agtatagatg gaagatgcag
     2461 taaaaaaaat actcacagat ataggagaag acccaacaag agaaggtctt cttgatacgc
     2521 ctaaaagagt aaagaaagcc tatgaatttt taacgagtgg ctatactacc gatattacaa
     2581 aattagtaaa cggagccatt tttgaagaac atgtagaggg tatggttatt gttagagata
     2641 tagaactcta ttcactttgc gagcatcact tacttccttt ctttggtaga gcacatgtag
     2701 cctatattcc aaataaaaag attataggta ttagtaaaat tccaagaatt gtagatgttt
     2761 ttgctcgcag actacaagtt caagagcgcc taacggatca agtcgccaat gcaattcaag
     2821 aaattctaga tcctctagga gttggcgtta ttatgaaagc aaaacatatg tgtatgatga
     2881 tgcgaggagt agaaaagcaa aattctgaat tactcacctc ttgtatgctt ggtgaattta
     2941 gagatagctc catcactcga aacgaatttt tagatctaat acgcacagga caataaaatg
     3001 tatctctggt ttaaggcact tcatatcatt tcacttatcg cttggtttgc aggaattttt
     3061 tatatctgga gattatttgt ttatcataca gaaactaatt cggaagatgt taaagcaact
     3121 ctcgcaatca tggagagaaa gctctacaca atcatcatgc gacctgctat gtttgctacc
     3181 ctcttttttg gatttggttt attttatcta caatggaatg ctttttcaaa ctccatttgg
     3241 atatggctca aaatattttt ggtcatactg gtagtaggtc aacatcatat ggcaaatttc
     3301 tatcgcaaga aattattaga aggtgtcgtc taccgttcta aaaaatttag aatcttaaat
     3361 gagataccaa cacttttaat gattcttata gtaatacttg tagtatttaa acctttctaa
     3421 tatatttatt cagaactagg ggaaacgttt cgtattggta aattatttag tctaagcttt
     3481 aaatcagtca ctatatcatc taatatattt tttacttgag gatagagtgg ctttgaaagt
     3541 tatgttagga ttattctatt atgattaaag tccaaaatct aactaaattt tattctgata
     3601 aacctgcaat caatggttta aactttcaaa ttaaagatgg agaaattgtt ggtcttctgg
     3661 gcttaaacgg atctggtaag acgacaacaa ttcgaattct aagtggtttt ttgattccga
     3721 atgatggaga ggtttgggtt gatgatctaa acttatttac cgacccctta gaaattaaaa
     3781 agaaaatagg ttatttacct gaaacaccac cgctatatga agattacact gtcagtggct
     3841 acttagagtt tgtcgcaaga ctcaaaggag tttcagatat acctacggag ctaacacgtg
     3901 tttgccagaa aacaaaccta gaagaagtaa aagataatta tatttctcat ctatctctag
     3961 gctatcgcaa acgagtagga attgctcagg ctattttagg gagccccaag attgttataa
     4021 tggatgaacc aatcagtggt ctagatccta aacaaattgt agaaattcga tatttaatta
     4081 aaagtttatc caaagaccat acagtcatca tttcaagtca tattctttct gaagtatacc
     4141 ttgtctgtga tcgatttctt tttttgcacc aaggaaaatt agtatatgat tattcaagag
     4201 aacaacttga aaaggaaatg agtaaaattt ctgtattgca tgttggactc cgcagttctt
     4261 cgcgtcttgc ctgtcaagcc tttctagaaa acattgacac cagtgcagaa gttaaactct
     4321 tagaagggga taatgactat tatctcttct atataaaacc gaaagatatt catatttacc
     4381 gagatcagat gcttcttggt ttacatacaa aggatttcaa gttagaactt ctcaaaaaag
     4441 aagatttaac gttagaacaa ttttttatcg attctatttg aattgaaata ggagcatacg
     4501 ttatgcaaaa tgtaaaatgg atttttttaa aagaatgtaa attatttttc aacacctata
     4561 tggcagctct tgttttaggt ggaacggctg tgttgaatgc acttctcgtt ttaatatcca
     4621 attttcatgg aactgaaaac tatgaagatg cagctctcat aacttttttt agtttcatgt
     4681 ctacaattat tatctctatg atgatacttg ctatgggaag catcgtcgaa gaaaaaaaca
     4741 aaggcacaat ggagctttta tacacagctc caatctcaga tcttgaaatt gtattaggta
     4801 aatttctatt tggtgcgttt gtatgtttga ttattaccat atttataaat ggactttttc
     4861 caatacttct ttactcttac tggaaagctc cgattccaat ttttgtttct ggaagtgtag
     4921 gtgtcttttt gctcggttgt tttatttatt ctgtggggct attcggcagt agcctcggaa
     4981 aaaaccaaat gatctctctt ttaattgcag ttttaattgt aatgatttta tgggttgttg
     5041 gctttttttc tcatttattc caagcaaaaa ctcgctccat tttatttcat ctacacatat
     5101 tctctcattt tatttctttt acgaaaggag tattaccact ttctggaatc gtttttttca
     5161 gttctggaat tttcacattt ctatatttaa cagttaaatc gctcgaatca agacgttggg
     5221 taggttaaaa tgaattataa tatttttttt ccaattctat ctcttctctc tctctttata
     5281 tattttttaa caatcgattt tttcacaacc tcattgagta aattgtcatg gaatcttttg
     5341 gggcttttgc ttattagttc tgatttaatt tatagaatta tacaatctag atctcaagat
     5401 tcggactccc aaaagaacat ccgccttttt gtttcaagtc taggtctaac atcctttttg
     5461 atctatcagg tgcgcgacta tttagatttt ccaattcccc ctggaggctc tgaacaagca
     5521 agtccatttc caaagataag agacttccta ttaattctaa tcattctaac gtctatcgca
     5581 tttttacttt acacaatcct actggaaata tctagacaat cagcaaacgc acaacacaac
     5641 ttacgccaaa aaaagaatac tatcttacaa aacactttat atagtttttt atggattagt
     5701 ccccttttag ttgcggttaa ttattttgcg gttcaaaaaa attacaattt tgatcttagc
     5761 tctagtggta aattttcatt ttccacaaca tctcgtcaaa ttttaaaaga tgtaaaaaag
     5821 gatattaaga taacagcctt ttttccgaga ccgctcgaat cagatgaacg aacaaaaaga
     5881 gaaagttttg cgattggctt aattcgtcct gaagtggaaa ttattttgga tcaactcaaa
     5941 tctttaaact ctcaaatatc cgttcaattc ataaatgcag atgtagaaaa agaattgatg
     6001 agtgatttta cacaagtatc aaatggagtc attttaatca gaagtttgaa atcaaataca
     6061 gatgtcggtt ccaatcctta tatggaagaa agagtcgttg tccaaactaa aaaggatttg
     6121 gaagatatag aacgcaaact tgtccaagca gtcattaatg ttgcgacctc acagaagaaa
     6181 ttttatttta caactggaaa tggagaacga tacggatcgg ctttccagag ccttccatcc
     6241 gaacagatta cacgactgac ttcaatgctt tcattctata attatggaat taaagaattg
     6301 ggatttaatg aaggctggcc cgaaaaaatt ccagatgatg cagatacgtt atttatcata
     6361 ggtccaacaa ctgcactttc tgaagtagct agaaacacaa ttttttccta tatagaaaaa
     6421 aaaggaaaac tctttattac tattgatcca gcgggtaacg aagattttgc gtggctctta
     6481 gagaaggctg gtcttagcat aaagaaggaa aatattagac aggtggaagg aagacctcag
     6541 attattgcaa ataaatttca accgcatcca atcgaggagc tgatttacaa taaagattta
     6601 ggagtatttt tcctagccaa tgcttatttt gaattaagtc cttctggcaa ttcaatattc
     6661 atttcaaata ctcttttaga atcaggtttt actgcatttg ctgacttaaa taaaaatgga
     6721 aaactagata aagaagaaaa acaaaatagt tttattttag gaacaatact tgttcctaaa
     6781 gaagatccag caaatcctgc taaaacagaa aatccatccc gaattataat ttattccgga
     6841 acaaactgga tcacaaatgg atttattgta gatcgattaa attctatact tgcagtaaac
     6901 agtgtaaact ggctcaatca atctcctcta acagaaaaga ttcttcccaa aaaagaagaa
     6961 gagcctgttg tatcgatcag tgattctcaa aaaatagtta tttggtctgt aggtttattt
     7021 atttatccag caattgtaat tttatcattg ggattttata caatctcaaa aaagagaaag
     7081 aaatgaagaa aattttaatg gttgctattg cgtttatcgt attagctttt gtattttttt
     7141 actcagaaga aagacatgaa gagggactca ccgagatcgc ttactggaaa attgagccag
     7201 atgaaattcg ttattatccg cctaaggatc attctgaatt ttctaatttt ataaaatcag
     7261 aactaacttt caaaagaatt aagactggct taaaaagttc accttatttt acagtagaag
     7321 gtatcgatcc ggaaagtaaa aagccatttc tatatgaagc cggctacaat gtgaaaaatc
     7381 tattcactga aatgtcggtt ttaaacgtta aatcaattaa tccagcaaat ccagatttac
     7441 taaagaaatt tcagatttca gagcaatcct ctcctgctct agaaatatat ttatcttcaa
     7501 agcctcagaa aaaaatctat cttggtgagc aaagtaacga taagaactat cgttacattt
     7561 taagtgataa agaagtctta acaacaattg gtcatgtttt tcaaaaattt tcacaaagtc
     7621 cttttgacct tagagataga caatatttac acttcggtga atttgaactt gctcgtatga
     7681 tttttcaatc agaaggagtt agaatcacaa ttgaaaatct tcctgaaact aaaaatggaa
     7741 ctcaaatgag tcgatggttt aaaacgacaa gcggtaagaa taaaatggat ccgagtcaag
     7801 gttcacaact tttttcatta ttacaagcat tcaaagccga tttatatcct gatgaaatag
     7861 aaggcgaagg atttttagtt gcgaaagaac ttacagctac ggaaacattt gcaaacttac
     7921 aacttatttt aacaaatgga atgcattata aaataaagct tttcccaaaa gtgattctga
     7981 aaaataaatc ctattatcct gttatgaaag aagtagataa gtttcaaata gaatcaacct
     8041 cttacaccaa tgaaacattg ataaatcaaa tgctggattt actaaaaaag attcaaacgg
     8101 atccagaatg gaaagatcca aaaggaaaat aaaatgctcg aactaacaaa aggggataca
     8161 cctttagtat ttccaaaatt tccaaatgaa gataaaatga ccgaatccga aatctgttgc
     8221 aagtgcacag gttgttgcag atacgtttct gttgatataa aaaagcctcg ctcaaaagat
     8281 acaattgatc tatatgtttg gtatttgctt cataaaaatg ttcaaattta tatagacaac
     8341 gacaaaggtt ggaatttact ttttataact acttgcacta aattacaatc ggatgggaag
     8401 tgtggaattt atccaactcg accgaaatta tgtaaagact attctccctc tggttgttct
     8461 cgcacaggta aggatcatac tcatttattt atgacacctg atgcactgct taattattta
     8521 gaagaagaga aagcaaagaa gggcaagaaa aagcaactaa aaagataaag taggtattct
     8581 atgggtcaat ctaatattgt tcacattgaa aaatggacta gcaaagtttc caattcagta
     8641 gaagaagaca tacgaagtga attaaaaaat ataatccaag aaaatccagc ctggggttgg
     8701 gagcaaatct caatccaaga tgtgtatgct tatgcattga atcaacttcc acctatttat
     8761 tcggaaaagg gtaagaaacc agaatacctt ctttcaattg aagaaattag aaatgcaatc
     8821 tataccgcca ttcgaaaaat agaagagaat ccaattcact atacaaaata atttctagat
     8881 aaaaaactac ttgaagggaa ccttataatt taaaaaaata gaagagtcat gcataaactt
     8941 tttatatctg cgctactaat aacttttatc tcctgcaata caaatatttc ccttaaaagt
     9001 aaaaacgaaa acaaagcaaa attgagtttt cctgaaatta catcgatatt atctgctacc
     9061 gattctgtgt attactttta tacacgtgat attgataaaa ctctaacaac aaattgtgga
     9121 gtggctacac ctggaacagt tggtggaaca acaggagcga ctggcacacc gggagcaact
     9181 ggcactactg gaacaaccgg aacaaccgga acaagcacct caagaagaat tatactaagc
     9241 cagcttgttt ttaagacagg cgaaacactt acaatgaaat ttcaatatga ttcaaaccaa
     9301 ttccaaggat ccttagacca acaacaagga tttactttaa caggtggaat ttttgggcat
     9361 actgtgaaag gaacacaagg aactgttaag tggtctggtc aaggagtagg ctatatagat
     9421 gaaacaaata atgctgctca aaccttaaca tatatgattg ttgatttaaa tttaagagga
     9481 acatttacag caaattcatc tagctctgga acaacaccag ttgagtgcaa tacaactgat
     9541 ggtattaatt gcacatcttc tgtttcaacg actaagtgct ttacccaaga ttatattaag
     9601 tgtgttgcaa acaataccgg tgggactgca attaccatct caggaagtat taattgtaat
     9661 gcacctaacg ttataccaca gtagtttata aatgattgat tttagtttag ttataccaac
     9721 ctttaacgaa gcagaaaata tctgcatctt aataccgcgg gtaatatcat tattatccgc
     9781 tgcgaatata agttttgaaa tcatcgtagt ggatgatgac tcaaaagatt taacatggaa
     9841 agcagtccaa gagagttttg caaatgagcc aagagttcgg gttctaagac gtgtaaacaa
     9901 aaaaggatta agctcagctg ttctagatgg aatggctatt gctaaaggaa aatctttcgg
     9961 agtgatggat gcagatttac aacacgacga aaatatcatt ccaaaaatgc ttcaagaact
    10021 agaaaaacac gatatcgttg tcgggagtcg agtcgtaggc gatggcggtt acggagaatg
    10081 gggacttatt cgaaaattaa tgagcaaggg agcaacgtta ctcgcaaaga taatgcttcc
    10141 tataccagtt ggcgatccaa tgtcgggatt ctttgtctta aagcgagaat tgtatgaaga
    10201 gttagttcca aaaataaatc cacgcggatt caaaatactt ttggaatttt tggcaagaaa
    10261 aaaaggaatc aaagcaaaag aagttggatt tgtatttaaa actagaattc atggagagac
    10321 aaaaatgtct ggttctgtaa tccagaatta tttagttgcg ttatacgata ttcgctttgg
    10381 tcatttaatt tcgattacat ttctaaaata cggtttcact ggtctaacag gcgtattcgt
    10441 aaatctattc ggacaatttt ttgctatgac tatttttcat ctaggaagct cccaagccta
    10501 ttatgaaaat ttcacaaaac cattcttagc tgttgcgttt ggatttgaac tcagtgtatt
    10561 gagtaattat atcatcaaca atctttggac atttagtgat tctaagatca atggtcttaa
    10621 gggaaattta ttaggctttt ttaaatttaa tatagtttct attgttggtt ttattattca
    10681 aatttctgtt tggagatttt cctaccaagt aattcaagat gaatttccac aattcttatt
    10741 taatggtcta acttatatat gtaattttgc tggaattgta ttggcaacag caggcaatta
    10801 cttcctaaat aaaaatttta catggcaaga aaaggatcgt taaacaatga atgaaattac
    10861 agttttgtta gatcaacata aatatattcg ttcaagatta attcaattta tcaaggaagt
    10921 ggcagcaact ccatatgcag aagaagcact tcattggagt atgccatttg gtaaaggtag
    10981 agctcatata gcatggcaaa tcatgcactg tgctgcgact tacgatcgtt atcttcactt
    11041 tcgaattcta aatgcagaac ctaaaaatcc agatttagtt aaggcttatg gaaatgggtc
    11101 agttccaaat ccaaaactaa tagtcaaagt agaagaaatt ttatctgctc ttgaacttac
    11161 tacaaaacct tattataatt attttgaatc tttaccaatt gataagctaa atgaaaagcc
    11221 tcatgaatca gcagatcgaa cctacaaaga aattttacat ctactgaatt ggcatgaagc
    11281 aagtcaccat ggacaagcac aaatcacatg gaatgccttt cgcgcttttc ggggacacat
    11341 ttaaaacgat aacagagact tatcacaata aataattcaa atttttactc ctaaaataaa
    11401 aaagtagaac ttttcgcgca catattctaa ttttctttct cctatattta ataaatacct
    11461 ttacctacaa gtatgttaaa actgtctacc accaagaaag acgatgggta gtatttatta
    11521 ttatgttttg tgactttgca ttatttttca atttcatcac gtttaagtct ctaaagattc
    11581 aaatattcat catttaaacg tatttaacca aagtcattaa actgaaatat gaatccatcc
    11641 atttgtcgtt ttgttatcgg tgtctacaca ttcaacaaac agagtgcaag tcggtgttaa
    11701 tctgttttgt ggataaggta taatatacta tagaggtatg aatatggaaa atgaatcgtt
    11761 acacgatata caagaaggta gtaacattat ttcaagttat aaaatctcct tccctagaaa
    11821 tgattatatt tacagtgaat ttagctcaac tattttaatc ccagatcaat actttaaata
    11881 ctttcaaaaa aaaatctata cgcataaagg agttcgctct tatattagtt accttctctc
    11941 gaaataccag atctatatca caaacggtct agtgcctagt tattctaatg taactacaaa
    12001 gtatcaggat aaaggtcaaa atttacataa gatagcattt cgcccaaagc cagaagattg
    12061 ggcagaactg aaactttatc gtttaacttt tggtatgtca atctctgcct ttttagtata
    12121 cctactaatt gcggactcta ccgattttgc tgaagccttt tccgactatc tcgagacagt
    12181 cggaatttca agggtcccaa attttaactt aagcgcaaag gtctttcttt tgaatgacag
    12241 gactaattat acaatagttt ttcaatatag aaaaagccga atccggcaaa acggataaat
    12301 ttctacttaa ctaagaaata aaaccaaatt gactttaaaa ctaaagggcg aagctttatc
    12361 tagacaattt cataaacaag attcactctt taaaaacaaa aaaattataa taagaaaggg
    12421 gctttcaccc ctttcaaatt catttagcag acttcagtca aatctatttt aatagttagc
    12481 tgttttctcc atgctaaatt cttgaaataa agttttttga ttgctatgtc ttgaagtgtc
    12541 aaagtgaatt ggatatttcc cactaaagca agcatcgcaa tagtttgaat ttttataatc
    12601 ttgttctact gctttatgca ttgactccat gcttaagtaa gcgattgaat ccacacgcaa
    12661 atactttcga atttcttcaa tcgagtgaga agaagcaatg agttcactat gggtcggaat
    12721 atcaattcca tagtagcaag gagaaacggt aggcggagca gagattctca tatgaatttc
    12781 ttttgctcca gcattccgta tcattttgac aattttacgg cttgttgttc ctctcataat
    12841 ggaatcatca atgataacaa ctcttttacc attcacgact tcacgtataa cgttgtattt
    12901 aattttagct ccaaagtcgc gaatcttttg atctggttca ataaaagttc ttcccacata
    12961 atgagaacga atgagtcctg tccgataagg aatacctgtt gcctccgagt aaccgagtgc
    13021 tgcaacgtta gccgaatcag gaacgggcat aacaacatct gcttcgactg gtaattcacg
    13081 tgataaatat tctcctaatt ttttcctgac tttataaacg gactctccaa aaatataaga
    13141 atctggtctt gaaaaataaa taaattcaaa aatacagaga ctactttttc ttggagaaaa
    13201 tgggaaatag gatttcattc caaaactatc caccacgacc atttctccag gagcaacatc
    13261 tcttacatat tgagtatctg taatatcaaa tgcacaagtc tcagaagcaa atacaattgt
    13321 tccgtcattt ttttgaccaa ttacaagagg acgaaaccca tttggatctc gaactgcaat
    13381 taacatttta cttgtaagaa ttaggagaga ataagcacct tccacttgtc tgagtgcgtc
    13441 tccgagtgcg tctaaaagaa ctttagattt agattttgcc attaggtgaa caatgacttc
    13501 tgagtctata gtagtttgga aaatactgcc ttcctcttcc atttttcggc gtatatcata
    13561 ggaattgacg agattcccat tatgtgccaa agctattcct ccaaggtgtg attctacccg
    13621 aactggttgt gcatttctca aaaagctatc accagtggtg gaatatcgat tatgtccgat
    13681 ggctgcatgc ccttttaact ctttcagttt ttcttgactg aataaattag caactagtcc
    13741 cattccagca tacctgtata ggtgttcgcc atctgttgat acaatcccac tggattcttg
    13801 accacggtgt tgcatggaat ataggcctaa ataggtgaaa ttagccgctt ccgaattatt
    13861 atacatccca aaaatcgcac attcttcttt tggtttgtca tcatgggttg tattaatatt
    13921 ttggatagaa ttgagggttt taagtttaga actcttagaa ttttccatag gtatacaatt
    13981 ttactctatt aaaaacatgc aaatcaattc caaatatatt ctagtcgaca ataaaaagag
    14041 tttagagctt gcagtagtaa acttagcaaa ctctagattg atcgcaatcg ataccgagtc
    14101 atccggttat tatacttacg tttcaaaagt ttgtttgata caaatttctg ccaatggaaa
    14161 gaactttatc tttgatccga tggcacaaat agacctaaaa gctttagatc cgattttccg
    14221 aaatccttcc attttaaaat tatttcactc agccgttgat gatatcaaag cgcttaagcg
    14281 agacttcgga tttcaattta aaaacatcgc agatactatg tattcttcca aacttcttgg
    14341 attagaacac aactcactca attacttagt agagttttat cacaaaatct ttttatctaa
    14401 gacagaacaa aaatcaaact gggagaaaag acctttagat agacaccagc ttcagtatgc
    14461 cgctctcgat actgcttatt tagagtcaat ttggaaccta atgaaagatg aattagagaa
    14521 aagaaatcta ctacaggaag cgatttccga atttgagaga atggcagatg aaccctatgt
    14581 tgccaaagaa aca
//
LOCUS       DAOQOS010000131        14559 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_2118_length_14559_cov_5.267099, whole genome shotgun sequence.
ACCESSION   DAOQOS010000131 DAOQOS010000000
VERSION     DAOQOS010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 14559)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14559)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14559
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2118_length_14559_cov_5.267099"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..674
                     /gene="hemB"
                     /locus_tag="PLF66_21240"
     CDS             <1..674
                     /gene="hemB"
                     /locus_tag="PLF66_21240"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002773072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HMZ66629.1"
                     /translation="FIQSIKKNFPEVTVITDVAMDPYSSDGHDGYVENGKILNDETLL
                     ILGKMALAQAEAGADILGPSDMMDGRVGYLRNILDKEGFKDVSIMSYTAKYASSFYGP
                     FRDALNSAPKAGDKKTYQMDSRNSREALLEAELDYEEGADFLMVKPALAYLDIIQTLK
                     NNFPIPIVAYNVSGEYAMIKAAAKQGWLDESKAMIECLTSIQRAGADIIITYFAKDFA
                     KLQQK"
     gene            678..1340
                     /locus_tag="PLF66_21245"
     CDS             678..1340
                     /locus_tag="PLF66_21245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate decarboxylase"
                     /protein_id="HMZ66630.1"
                     /translation="METKHYPPPWHLKGQGFILPFLGNKTALLDKGFISESDKQNFRG
                     GLGAIMIVNYDSSNVGPYYELLFIPGDFYYENSPKKVYKKITKIFVSTDISIQEGRLN
                     WAIPKEFANFNWIKKNNNTLISVESSLGEPILKAELNQKFFSFPLTTKIYPITLLQKS
                     EDDRLISTQFYGSGYGKMSSILEWEANPKYFVDIVSISKFFLGISAHQFNITFPLPEI
                     LK"
     gene            1343..2554
                     /locus_tag="PLF66_21250"
     CDS             1343..2554
                     /locus_tag="PLF66_21250"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HMZ66631.1"
                     /translation="MNNEDLLKEVEKLKKELDSVREQSRRKTIIFSAISHEIKNPITA
                     IIGMINLLLEENPSDSQLQILKTMKFSADNLLKLLNTFLDYSRIESGKFELEETEFNI
                     NDFLLNIKQMYFEVARQKNLVLELKVDESLPPIVIGDSLRLTQLLTNLISNAIKFTKE
                     GSVLIDAKNKGAKANLIEIEFSVEDTGIGIPKEKLDSIFDVFNQGESNINRQFGGSGL
                     GLTITKRILELMESKIIVKSEPNKGSRFSFCLYLRTPLPTKNVPRLDPIPLIPQKSLR
                     GLKILLVDDNISNQKVTERYLQKWEIEVDIAENGLVCLEKVDQKDYNLILMDIQMPEM
                     DGYEAAQKIREKKDPKYKGIPIIALTASTLLDTSDKVKSAGMNGYIAKPFSPTELFNI
                     IEKYSSSKNSQ"
     gene            2606..2682
                     /locus_tag="PLF66_21255"
     tRNA            2606..2682
                     /locus_tag="PLF66_21255"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2640..2642,aa:Met,seq:cat)
     gene            2702..2986
                     /locus_tag="PLF66_21260"
     CDS             2702..2986
                     /locus_tag="PLF66_21260"
                     /inference="COORDINATES: protein motif:HMM:TIGR04453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-rich protein"
                     /protein_id="HMZ66632.1"
                     /translation="MKLIISSLLVFSLFSSLYALDPKCAPACDRFLECAKEMNKGKSA
                     TAAEIKRMKDGCLNACKRNTKAVLACYESSQETCNEFALCISKSYSATKK"
     gene            complement(3061..4188)
                     /gene="thiH"
                     /locus_tag="PLF66_21265"
     CDS             complement(3061..4188)
                     /gene="thiH"
                     /locus_tag="PLF66_21265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010574278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HMZ66633.1"
                     /translation="MKKIEFLEVFNQYSFKDCIAEIESKAKEDVETSLHKAYKKNNLN
                     FQNYLSLISPASDSYLEAMASLSKEITQERFGKTIQLYMPMYLSNECRSSCLYCGFSF
                     ENKIKRKTLSFSEIRRESEILKNKGIEHILILTGEDYSKTSISYLLEAVKILKEYFSS
                     ISIEIYPMDVDDYKKLIERGVDGLTLYQETYDKEIYKKYHLRGVKKNMEYRLNGPDRG
                     GFAGFRKIGIGALLGLAETLGELYFLGLHLDHLYKNYWQSFMQVSLPRMRPAEGEFNS
                     VIPVNDREFLKYIFALRIYFPDLGIALSTRERIKLRDNLIGLGITTMSAESKTEPGGY
                     SGSGELEQFETEDKRTLQEIVNVIRRKGLDPVLKDFDRALI"
     gene            complement(4166..4975)
                     /locus_tag="PLF66_21270"
     CDS             complement(4166..4975)
                     /locus_tag="PLF66_21270"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020770697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HMZ66634.1"
                     /translation="MEDNEDYLQMGGKKFRSRLFVGTGKFSSSKEMKNAIESSGTEIV
                     TVALRRVDLEKSNDDILSSIDRNKILLLPNTSGARNAVEAVRLAKLARSLGAGDWVKL
                     EVVPDPVYLLPDPIESLKAAIELVKEGFKVMPYINADPILCRHLEDAGCVCVMPLGSP
                     IGSNLGIKNRANIEMIIDQSNIPVVIDAGLGEPSHAAEAMEMGADAVLVNTAIAIAEN
                     PSKIAEAFKMATIAGRLSFLHRGTRSLGQVRINANASSPLTGFLNEEDRVS"
     gene            complement(4991..7756)
                     /locus_tag="PLF66_21275"
     CDS             complement(4991..7756)
                     /locus_tag="PLF66_21275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HMZ66635.1"
                     /translation="MYLKSMNIVGFKTFADETDVVFDPGFTAVVGPNGSGKSNIVDAL
                     RWVLGEKSAKGLRGDKMEDVIFHGSETRDPAGFAEVAVNIDNTSKTLPIDFPTVKITR
                     RLFPDLTNEYYINNSRVLRKDVEKLLMDTGIGKASYSIMEQGRVEAILNAKPEDRRAI
                     FDEAAGISKFKLDRLETEKKLELTTQNLLRITDIMSSMEKELELKSKQAERAKAYFDL
                     KNKVTEADKNLRYLKLKSLKSRSKKVDAELTEVKRKNEELMARMSDDAKLIEEREKFR
                     YEMEQKIAEIDKKLLDFLSQKEIQREKIEKNKSIIHEFDQRIDELTTNLESEKTRMEN
                     AEAEYNQLLALSDNLAKMIQMTLENVERFTSDKKQLEINIEEELKNIQSYQDSIASND
                     KKHNVLREKLKDSILSLINQIEEKKRDAEKFEGRREELRKYLILSLDESIEKINEILS
                     SELNSERITQILKELNLTSYRENLKDFIEMEDVFRDILLDKDGLLAKKESIDSEIEDL
                     ILENENLSRKIKDANSNIESLRYALEEKKEEIVKLEKSILEMEGKTASALDARDKELR
                     IKHEAGDRIANFEKSIEALQTKKVTYQEEVKLLEEQIEKSYTEFLGMSKTLEAEKEQL
                     KELFSEIQNLKSSSQADQEEFKSIIPVMNDLERKASALRVQLDTFNEELYNDYSMSDV
                     ELEEEKSSLSLKQEAEDSRLRELKSEMQLLGSINPLAVEEYNNVKEIYDHHKNQKEDI
                     EKSKQDILDVLKNINSESEKLFIETFEVIKKNFQETFSTLFNGGHATIELTEKEDRLN
                     SGVEIIAEPPGKHVQNLKLLSGGEKSMTVIALLFAIYMVKPSPFCFLDEIDAALDEVN
                     KIRFCQILDKFKDVSQFVVITHAPPTISRANAIFGVTSAEPGVSKVVSLKIEEAKQFA
                     KKFQEAV"
     gene            7927..9723
                     /locus_tag="PLF66_21280"
     CDS             7927..9723
                     /locus_tag="PLF66_21280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000019140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF115 domain-containing protein"
                     /protein_id="HMZ66636.1"
                     /translation="MSLINLEDLILKKPYLKLYLNSIQNGNENYKLSQAKGKEEYYLT
                     FQNKNLTSAVAPLTASLRMIGESPIKETELVVVLGLGNPHLITELNNKLQQNQIIIFI
                     DSSEDVLNPLWEKFLYPTLSIPGRHIFLGDKLLHLLWSYIESLPIERLSGIKVFKNQP
                     SVQLDVNFYEEIHQKINQLFSSKMSDLLTKFEFERIWVRNSIINLIQFNRNEIPCYRF
                     KDYLGTLENIPAMLVSAGPSPRSQCPFIKKNRDKVFLIACDTALKVLLKFNIIPDAVM
                     TLDAQTHSYFHFLGEDLTEIPIFADFVTSPRLLRSLSFKSIVHSLTAKFQVDAYGKPF
                     REVTAGGEIVEEKLGSIGEVQSGGSVATSAFDIIRQMGCSPIFLVGQDLAYTGREIHS
                     TGTHHNEKWLTIINRKKSLEKINEEIVRKRETTFVKAANGGQVLTDYVLGLYKHWFEE
                     SANSTNLPIYNINENGSYIENLKNISVATAEDVFLNISEHKYPWKNLNPWKKDNSEKM
                     ENKIQKEEFLNDLNHLKQFIDCSHHLESQQFTEELLDKVKMIPYLKQMIRKTEIYVKR
                     HELELTENRKKDILVSSILKEIRFLKKGILIA"
     gene            complement(9720..10019)
                     /locus_tag="PLF66_21285"
     CDS             complement(9720..10019)
                     /locus_tag="PLF66_21285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015678495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66637.1"
                     /translation="MKPAKINPSELNNITKKIIEKSDEAIGLFVYNGFRIQVSKYKMD
                     GGSRVRILYNKRRAKGLCIICVSKVTKINPITGKLYRLCETHRTSIDKERGRKKG"
     gene            complement(10335..11129)
                     /locus_tag="PLF66_21290"
     CDS             complement(10335..11129)
                     /locus_tag="PLF66_21290"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HMZ66638.1"
                     /translation="MSFPFHLKLGVFEISAHLIFETLAFIIGFQYYLFLRKSSKDQLN
                     SEERFWILIAVCLGALFFSRLIAVMEHINLIQKNTPISFYYSQKTIVGGLLGGLIAVE
                     VTKKLLGITKSSGDLMTYPILLGMIIGRIGCFLSGLEDGTYGVETNTFLGVDFGDGLK
                     RHPTQLYEIIFLLILWILIYLLTIPKFWNFIGFKNNRLDNYTTLADGMKFKIFLFSYL
                     IFRFFIEFIKPVSKFAFQISFIQIACLIGIFYYLILAFVKINFFRR"
     gene            complement(11135..12265)
                     /gene="mqnC"
                     /locus_tag="PLF66_21295"
     CDS             complement(11135..12265)
                     /gene="mqnC"
                     /locus_tag="PLF66_21295"
                     /EC_number="1.21.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016560779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic dehypoxanthinyl futalosine synthase"
                     /protein_id="HMZ66639.1"
                     /translation="MLLTSKTEDKTDKILLKALDGERITSDEALLLYEEGDFLKIQFV
                     ARAIREKFLSHKYASYTMFRVVNYTNYCNVECSFCSFMDEIGSGKGYVLSKEQILEKM
                     EFAAKENADQMFLQGGVYPELKFDYYLDVLKTVKERFPQMHIRAFSPVEIINLERITG
                     LSLREVLKTLKDAGLDSVPGAGAEILTERMRNIISPKKASVTEWVRAMETCHEVGLLG
                     SANVVFGSEETKAEVIEHLDVIRNLQDRSKGFLSFIPWTFQPQTKRFKIRMVPTHEYL
                     KVLGICRIFLDNIPHLETSVMVLGKGVGQLALYSGADDVSSVVIEENVLRSYGLKSEE
                     EAIRFLSGAGFIPKRRDLTYNYEKYPEYIPTGNEKSYMNLGT"
     gene            12357..13721
                     /locus_tag="PLF66_21300"
     CDS             12357..13721
                     /locus_tag="PLF66_21300"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020777457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HMZ66640.1"
                     /translation="MKQYILTLLLFLITIQINSDPIRKYKIVIDPGHGGVKQEPLEIF
                     GDKYDTITRRFLENYKDGASYAKANRTEMEIVLGVGKELKEILDLTKTKKGFQKFKSY
                     VKLFSDSEVPWIKIDSVMTRTDSYKDRNYREKEDKNQLYRLYDFPDFKTGKNRLGRIS
                     QINKEKPQLVVSLHINAMNSTPDSGGMGAVIAPSYQTFELLKRISEKKAQPEEFLKTP
                     WKNWMLFESKWSRLENAMADAWIYFNGYWPTQDGSETNKERFEGYRANMITWRYRDDI
                     GWEKKINKKEGPYAIDHSNFRAVGKFWDRERGKPELMRREGGPEGYGGDNLYASNELL
                     RFLQYGLRMQVNEDKDNYKEPNKILLPFISTYSVPTYINAISAYLELGEITSDRDMYF
                     VTTKKRKTAICLAVGIYSLFNGLEIKQMETLYTPKGKRIDFEKYIGSTGQSYFDEVTT
                     DKNE"
     gene            13714..14469
                     /locus_tag="PLF66_21305"
     CDS             13714..14469
                     /locus_tag="PLF66_21305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016547167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66641.1"
                     /translation="MSKPTPLKEKEFGLLILAYRKSLIKRYSRENLKRFKEFEGIDQS
                     TVDKLTYYFLELLYPELEERLKLDQAFDSLASFVHNPDKMFGLLGSVGTILYKFGKQF
                     FSATKAGISALRSYVTAHKFESILYENAKKMIQEGKDIEEDTNFNKLIASIPRKEADD
                     FRHQIVNLFSTLSRKELLQKILEVMDYVIDKMKSKPETYSKDDIDGISLGREIIIHGK
                     EVFTNLSQEQIDLILKGIDTIEKDFYYRALETK"
     gene            complement(14472..14558)
                     /locus_tag="PLF66_21310"
     tRNA            complement(14472..14558)
                     /locus_tag="PLF66_21310"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14522..14524),aa:Leu,seq:caa)
BASE COUNT         4912 a         2554 c         2247 g         4846 t
ORIGIN      
        1 aattcattca aagtattaag aaaaatttcc ctgaggtaac agtcattacc gatgttgcaa
       61 tggatcccta tagttcagat ggacacgatg ggtatgtgga aaatggaaaa attttaaatg
      121 atgaaaccct ccttatcctc ggtaaaatgg ctcttgcaca ggcagaggct ggagcagata
      181 tattgggtcc ttctgatatg atggatggac gtgttggata tttaagaaat atcttagaca
      241 aagaaggatt taaagatgta tctatcatgt cctatacagc taagtatgcg agtagttttt
      301 atggaccgtt tagagatgct ttaaattcag ctccgaaggc tggtgataaa aagacatatc
      361 aaatggattc aagaaattct agagaagcct tattagaagc agaattagac tatgaagaag
      421 gtgcagattt tcttatggta aaacccgcat tagcttattt ggatataatt caaactttaa
      481 aaaacaattt tccaattcct attgttgcct ataatgtgag tggagagtat gctatgatta
      541 aagcagcagc aaagcaagga tggctagatg aatcgaaagc tatgatagag tgcctaacgt
      601 caattcaaag agctggtgca gatataatca taacatattt tgcaaaggat tttgcgaaac
      661 tacagcaaaa ataaattatg gaaacaaagc actatccacc accttggcat ttaaagggac
      721 aaggtttcat tctacctttt ttgggaaaca aaactgctct actcgataag ggctttattt
      781 ctgaatccga taaacaaaat tttcggggtg gacttggtgc tatcatgatt gtaaattacg
      841 atagttctaa tgtaggtcct tattatgaac ttttatttat tcctggagat ttctattatg
      901 aaaattctcc aaagaaagtt tataaaaaaa ttactaagat ttttgtatcc actgatatct
      961 ctatccaaga aggtagactg aattgggcaa ttcctaaaga atttgcaaat tttaattgga
     1021 tcaaaaaaaa caataataca ttgatttccg ttgaatctag tttaggggag cctattttaa
     1081 aagctgaatt gaatcaaaaa ttcttttcat ttccacttac tactaaaatt tatcctatta
     1141 ctcttttaca aaagtcagag gatgatcgac tcattagcac tcagttttat gggtctggat
     1201 acggaaaaat gagttctatt ctggaatggg aagcaaatcc caaatatttt gttgacattg
     1261 tatccatttc aaaatttttt cttgggatat ctgctcacca atttaatatt acatttcctt
     1321 tgccggagat tctaaaataa tcatgaacaa tgaagacctt ttaaaagaag tggaaaaact
     1381 aaaaaaagaa ttggattctg ttcgcgagca atcaagacgt aaaacgatta tcttttcagc
     1441 tatcagccat gaaataaaaa atccgataac agcgattata ggaatgatta atctactttt
     1501 agaagagaat ccttccgatt cccagttaca aattttaaaa acaatgaaat tctcggctga
     1561 taatctttta aaacttttaa atacattttt agattattct agaatcgaat caggtaaatt
     1621 tgaattggaa gaaactgaat ttaatataaa tgactttttg ttaaatataa aacaaatgta
     1681 ttttgaagtt gctcgtcaga agaatttagt cctcgaatta aaagtagatg aatctttacc
     1741 gcctatcgtt attggagatt ctcttaggct cacacaactc ttgacaaatc tcattagcaa
     1801 cgcgattaaa ttcacaaaag aaggatcggt tttaattgat gcaaaaaaca agggtgcgaa
     1861 agcgaatcta atagaaatag aattctcagt ggaagatact ggaatcggta ttccaaaaga
     1921 aaaattggat tcaatttttg atgtctttaa tcaaggcgaa tccaacataa atagacaatt
     1981 tggaggttca ggtctcggtc tcacgattac aaaaagaatc ctagagctta tggagagtaa
     2041 aattatagtt aaaagcgaac ctaacaaggg ctctagattt tcattttgcc tctatctccg
     2101 aacccctctt cctacgaaaa atgtcccccg tttagatccg attccactca ttccacaaaa
     2161 aagtctacgt ggattgaaaa ttcttttagt agatgataac attagcaatc aaaaagttac
     2221 ggaaagatac cttcaaaaat gggaaattga agtagatatt gcggaaaatg gtctcgtctg
     2281 cttagaaaaa gtggatcaaa aagactacaa tttaattctc atggatatac aaatgcccga
     2341 aatggatggg tatgaggcag cccaaaaaat aagagagaaa aaggacccaa aatataaagg
     2401 tataccgatc attgcactca ctgcctctac acttctagat acaagtgata aggtaaaaag
     2461 tgcaggaatg aatggatata tagccaaacc tttttcccct accgaattat tcaacataat
     2521 agaaaagtat tctagttcaa aaaattcgca ataatgtttt tgacactaat agcattttaa
     2581 aagtaaggtt aaactaggtt tttacggcga catagctcag ctggttagag catcggaatc
     2641 ataatccgca ggtccggggt tcgaatccct gtgtcgctac caaaccttca aggagacaag
     2701 tatgaaactc attatttcct ctcttctcgt tttttccctt ttttcttccc tctatgcact
     2761 agatccaaag tgcgcacctg cctgcgatcg gtttttagaa tgcgcaaaag aaatgaataa
     2821 gggtaaatct gcaacagcag ctgaaattaa aagaatgaaa gatggatgtt taaatgcctg
     2881 caagagaaat acaaaagctg tattagcatg ttatgaatcc agccaagaaa cctgtaacga
     2941 atttgcactt tgtatttcca aaagttactc tgctactaaa aaataattct taaatctttt
     3001 cacataaaag ataactcaat gtttagggaa gcaaagagtt atcttttttt atttcaaaat
     3061 ctagatcaaa gcccgatcaa aatcttttaa aacaggatcc aagcccttcc ttcgaataac
     3121 attgactatt tcctgcagag ttcttttgtc ttctgtttca aattgttcca actccccact
     3181 cccactgtat ccacctggct ctgtcttaga ttcagcagac attgttgtaa tcccaaggcc
     3241 aattaaatta tcacgtaatt tgattctttc cctggtagag agggcaattc ctaaatctgg
     3301 aaaataaatt ctgagtgcaa aaatatattt taaaaattct ctatcattca ctgggataac
     3361 agaattaaac tcaccttctg ctggcctcat ccgtggaaga gatacttgca taaaactttg
     3421 ccagtaattc ttatacaaat gatctaagtg taaacctaga aaatacaatt ctcctaacgt
     3481 ttccgccaaa cccaagagag cccctattcc aattttccta aaaccggcaa aacctcccct
     3541 atcaggtcca ttcaatcggt attccatatt tttttttacg ccacgtaaat gatatttttt
     3601 gtagatctct ttatcatacg tttcttgata gagagtcaga ccatctactc ctctttcaat
     3661 taacttttta taatcatcca catccattgg ataaatttca attgaaatag atgaaaaata
     3721 ctcttttaaa attttaacag cctccaatag ataagaaatt gaagtttttg aatagtcttc
     3781 tcctgttaag attaatatat gctctatacc tttatttttt aatatttcgg attctcttcg
     3841 aatttcagaa aaggataaag tctttctttt tattttattc tcaaaactaa atccacaata
     3901 caaacaagaa gatctgcatt cattggaaag atacattggc atatacaatt gtattgtttt
     3961 tccaaatctt tcttgcgtaa tctcttttga aagacttgcc attgcttcca aataggaatc
     4021 agatgctggg cttataagag ataaataatt ttgaaagttt agattgtttt ttttgtatgc
     4081 cttatgcagt gaagtctcta catcttcttt tgcctttgat tctatttctg caatgcaatc
     4141 tttaaaacta tattgattaa atacttcaag aaactctatc ttcttcattt aaaaatcctg
     4201 tcaatggact cgaagcattt gcattgattc taacctgtcc taatgatcta gttccacgat
     4261 ggagaaatga aagtctacct gcgatagtcg ccatcttaaa agcttctgca attttagaag
     4321 gattttctgc aattgcaata gctgtattga ctaacacagc atcggctccc atttccatag
     4381 cttccgcagc atgtgaaggc tcacctagac ctgcatcaat tactactgga atattagatt
     4441 gatctatgat catttcaatg tttgctctat ttttaatacc aagattggat ccgataggag
     4501 aaccaagagg cataacacaa acacaacctg catcctctaa atgtctgcat agaataggat
     4561 cagcatttat ataaggcatt actttgaatc cttcttttac taattcaata gcggctttca
     4621 gtgattcgat tggatctgga agtaaataaa caggatcagg aacaacttct aattttaccc
     4681 agtcacccgc ccctaaagat cttgccaatt ttgctaatcg aactgcttct actgcatttc
     4741 tggctccact tgtattaggt aaaagtaaaa ttttatttcg atcaatcgaa gagagaatat
     4801 catcatttga tttttcaagg tcaactcttc taagtgcaac cgttacaatt tcagttccag
     4861 aggattcaat ggcatttttc atttcctttg aagaggaaaa ttttcctgtt cctacaaata
     4921 atcgggagcg aaacttcttt cctcccatct gtagataatc ctcattatcc tctatttgaa
     4981 tcatagatct ttatactgcc tcttgaaatt ttttagcaaa ttgtttagct tcttctatct
     5041 tcaaagaaac aacttttgaa acacctggct ctgctgaggt tactccaaaa attgcattcg
     5101 ctctcgaaat agttggaggt gcgtgagtaa taacaacaaa ttgactaacg tctttgaatt
     5161 tgtccaatat ttgacaaaac cgaatcttat ttacttcatc taacgctgca tcaatctcat
     5221 ccaaaaagca gaatggcgat ggcttcacca tgtagattgc aaaaagaagt gcaatcacag
     5281 tcattgactt ttccccacca gataataact ttaagttttg aacatgtttt cctggtggtt
     5341 cagcaataat ctctactcca gaattgagtc tatcttcttt ttcggttaat tcaattgtag
     5401 catgcccacc attgaaaaga gtagagaaag tctcctgaaa atttttctta atcacttcaa
     5461 atgtttcaat aaataacttt tcagattcac tgtttatgtt ctttaaaaca tctagaatat
     5521 cttgcttact cttttcgata tcctcttttt gatttttatg gtgatcataa atctctttca
     5581 cattattgta ttcttcaaca gctaaaggat tgattgaacc aagtaattgc atttccgatt
     5641 ttaattctct cagtctagaa tcttccgctt cttgttttaa actaagagag gacttttctt
     5701 cttctagttc tacatcactc attgaatagt cattgtatag ctcttcatta aaagtatcga
     5761 gttgaactct aagagcggat gcttttctct ctaaatcatt catgacgggt atgattgatt
     5821 taaattcttc ctgatctgct tgagaggaag attttaaatt ttgaatttct gagaaaagtt
     5881 cttttaattg ttccttctca gcttctaatg ttttactcat tcctaagaat tcagtatagg
     5941 acttttctat ttgttcttct aaaagtttta cttcttcttg ataagtaact tttttagttt
     6001 gaagagcttc aattgatttt tcaaaatttg caattctatc accagcttca tgctttatac
     6061 gaagttcttt atctcttgca tctaaagcag aggcagtctt tccttccatt tctagaatgc
     6121 tcttttctag ctttacaatc tcttctttct tttcttctaa tgcatatcga agagactcta
     6181 tattagaatt tgcatctttt atttttctag atagattttc gttttctaaa atcaaatctt
     6241 caatttcaga atcaatactt tctttctttg caagtaatcc atctttatcg agtaagatgt
     6301 ctctaaaaac atcttccatt tcaataaaat cttttagatt ttctcgataa gaggttaaat
     6361 ttaattcttt cagaatctga gttattcttt cagaattcaa ttcactcgat aatatttcat
     6421 taattttttc gatactctca tctaaagata aaatcagata cttacgaagt tcttctctac
     6481 gtccttcaaa cttctcagca tctctctttt tctcttcaat ttgattgatt agagatagaa
     6541 tactatcttt cagtttttct ctcaatacat tatgtttttt gtcatttgat gctatactgt
     6601 cttgataaga ttgaatattt ttaagttctt cttcaatatt gatttctaat tgtttcttat
     6661 cagaagtaaa acgctccaca ttttcaagag tcatttgaat catcttggct agattatcgc
     6721 ttaacgctaa gagttgattg tattctgctt ctgcgttttc cattctagtt ttttcacttt
     6781 caagatttgt agttagctca tctattcgtt gatcaaactc atgaataata cttttatttt
     6841 tttcgatttt ttctctttga atttcctttt gggatagaaa atctaaaagc tttttatcga
     6901 tctctgcaat cttttgctcc atttcatatc tgaatttttc tcgttcttca atgagttttg
     6961 catcatcact catcctagcc atgagttctt cattctttct ttttacttca gtaagctcag
     7021 catctacttt cttagatctg gattttaaac tcttcaattt cagatagcgt aaatttttat
     7081 ctgcttcagt tactttattt tttaaatcaa aataagcctt tgctctttca gcctgtttgc
     7141 tttttaactc taattccttt tccattgagg acataatgtc tgttatccgc aaaaggtttt
     7201 gagtcgttaa ctctagtttc ttttcagttt ctaatcgatc taatttgaat tttgaaatac
     7261 ctgctgcttc atcaaaaatt gcccttctgt cttcgggctt agcatttaga attgcttcca
     7321 ctcttccttg ttccataata gagtaacttg cttttccaat gcctgtatcc attagaagtt
     7381 tttctacatc ttttcttaga actctagaat tattaatata atactcattt gttaaatcag
     7441 gaaacagtct acgagttatt tttactgtcg gaaagtcaat tggtagtgtt ttactggtat
     7501 tatcgatatt aaccgcaact tcggcaaatc cagctgggtc tctcgtttcc gagccatgaa
     7561 agataacatc ttccatttta tctcccctaa gtccctttgc agatttttct ccaagcaccc
     7621 atcgaagtgc atctacaata tttgactttc ctgacccatt tggacctaca acagcggtaa
     7681 atcctgggtc aaatactacg tctgtctcat ccgcaaacgt tttgaatcca acaatattca
     7741 tactctttaa atacataatt ccctcaataa tacccttctt cactcaattg aatttttcag
     7801 tgattttatt gctgtaaaat caaactcaat ctaataaaaa ggttgaaatg aatggatcgg
     7861 ttcatttttg ttttttaaaa ccgattgacc aaatgtctag ggtgtacttt ataagaagtg
     7921 ggtattatgt cactaataaa tttagaagat ttaattttaa aaaagcctta tctcaaactc
     7981 tatctaaatt ctattcagaa tggaaatgag aattataaat taagtcaggc taagggaaaa
     8041 gaagaatact acttaacatt tcaaaataaa aatttgacca gtgcggtcgc accacttacg
     8101 gcaagtcttc gcatgatagg cgaatcccca atcaaggaaa cagagctggt agttgtttta
     8161 ggtttaggca atccacattt gattacagaa ctaaataaca aattacagca aaatcaaatc
     8221 ataatattca tagactcttc tgaggatgta ttaaatcctc tttgggaaaa atttctctac
     8281 ccaactcttt cgattcctgg taggcatatt tttttaggtg ataaattatt acacctactc
     8341 tggagttata ttgaatcact tccaattgaa agattatccg gaataaaagt ctttaaaaat
     8401 caaccaagtg tccaactcga tgttaatttt tatgaagaaa ttcatcaaaa aataaatcaa
     8461 ctcttctctt ctaagatgag tgatctatta acaaagtttg aatttgagag aatttgggtt
     8521 agaaattcaa tcattaatct aattcaattt aatcgaaatg aaattccatg ttatagattc
     8581 aaagattacc tcgggacact ggaaaatatt ccagcaatgc ttgtttcagc aggtccaagc
     8641 ccaagaagtc aatgcccatt tattaaaaag aaccgagata aagttttcct aattgcgtgt
     8701 gatactgcct taaaagttct actcaaattt aatattattc cagatgcagt aatgacgtta
     8761 gacgctcaaa ctcattcata ctttcatttc ttaggagagg acttaacaga aattccgatt
     8821 tttgccgatt ttgtaacatc acctagactc ctaagaagtc tttcttttaa atctatcgtt
     8881 cacagtctta ctgcgaagtt tcaagttgat gcttatggta agccttttag agaagtaact
     8941 gccggcggag aaattgtaga agaaaaactc ggatctattg gcgaggttca atcaggtggt
     9001 agtgtagcga ctagtgcttt cgatatcatt cgccaaatgg gatgttcgcc tattttttta
     9061 gtaggacaag atttagctta cacaggtaga gaaattcatt ctactggaac ccatcacaac
     9121 gaaaagtggc taacgatcat taatcgcaag aaatcattag agaaaataaa tgaagaaata
     9181 gttcgaaaaa gagagactac ttttgtaaaa gcagcaaatg gaggacaagt cttaactgac
     9241 tatgtgcttg gtttatataa gcattggttt gaagagtcag ctaattctac taatctacca
     9301 atttataata ttaatgaaaa cggatcctat atcgaaaatt tgaagaatat atcagttgct
     9361 actgccgagg atgttttttt aaacatttcc gaacacaaat atccttggaa aaatctaaat
     9421 ccttggaaga aagataattc ggaaaaaatg gaaaataaga tacaaaaaga agagtttcta
     9481 aatgatctca atcacctaaa acaatttata gattgttctc accatctaga atcccagcaa
     9541 ttcacagaag aattattgga caaagtcaaa atgatacctt atttaaaaca aatgattcga
     9601 aaaacagaaa tttatgtaaa gagacatgag ttggaactaa cagaaaatag aaaaaaagat
     9661 attttagtta gttccatatt aaaagaaatt cgttttttga aaaaaggaat cttaatcgct
     9721 tagccttttt ttcttcctct ttccttatca atcgaagttc tatgagtttc acaaagacga
     9781 tataactttc ctgttatagg attgatttta gtaactttag atacacaaat aatacagagt
     9841 ccctttgctc ttcttttatt gtaaagtatt ctaacacgac ttccgccatc cattttatac
     9901 ttacttactt gaattctaaa tccgttatac acaaataacc caatcgcctc atccgatttt
     9961 tcaattattt ttttggttat gttatttaat tcgcttggat ttattttagc aggcttcatt
    10021 ctatccttct taagtctaac ttttttttat ttgttagaca atggaaagtg atttttgttt
    10081 ttattagatg caaatttatt tatctaagga aaacaaaaat aatttgttat aaatagattc
    10141 gcctatacgt taactataag ctaaataaat cagaataaaa tttatacatt acttcttctt
    10201 tcaaaatgca atgatttatt tttaaatgga atacgatcca aagatagata agaaatgaaa
    10261 ctattttaaa tgtatttagc atatgtagat tagtatatat ctttcttggt gtctaatcta
    10321 taaaaatagt ttttttacct tcgaaagaaa ttaattttta caaatgctaa aattaaataa
    10381 tagaatattc cgatcagaca tgcgatttgg ataaatgaaa tttggaatgc aaattttgaa
    10441 actggtttaa taaactcgat gaaaaatcga aatattaaat aggaaaataa aaaaatttta
    10501 aacttcatcc catctgcaag agtagtataa ttatcgagcc tattattttt aaatccaata
    10561 aaattccaaa actttgggat agttaacaga taaatcaaaa tccataggat taataaaaat
    10621 atgatttcat ataactgagt cggatgacgc ttcagtccat ctccaaaatc tacccctaag
    10681 aaagtattgg tttccactcc ataggttcca tcctctaaac cagataaaaa acagccaatc
    10741 cgaccaataa tcattccaag tagaattgga taagtcatta aatcaccgga agattttgtt
    10801 atccctaata atttttttgt gacttcgaca gctattaaac cacctagaag tcctcctact
    10861 atcgtctttt gtgaataata aaaagagata ggagtattct tctgtatcaa attgatatgc
    10921 tccatcacag caatcaatct ggaaaaaaaa agagcaccta aacacacagc aattaaaatc
    10981 caaaatcgtt cttcagaatt taattgatcc ttgctgcttt ttctcaaaaa taaataatac
    11041 tgaaatccaa tgatgaaagc aagggtctca aaaattaaat gtgcagaaat ctcaaatact
    11101 ccgagcttta aatgaaaagg aaaactcact ttttttacgt gcctaaattc atgtaactct
    11161 tttcgtttcc tgtaggaata tattctggat atttttcgta gttataggtt aagtcacggc
    11221 gtttaggaat aaaacctgca cctgatagaa aacggattgc ttcctcttcc gattttagac
    11281 cataagaacg taaaacattt tcttcaatca caacagagga gacgtcatcc gctccactat
    11341 agagagccaa ttgaccaact ccttttccta gaaccataac ggaagtttca agatgaggaa
    11401 tattatctaa aaagattcta catattccaa gaacttttaa atattcatgt gttggaacca
    11461 tacgaatttt aaatcgtttt gtttgtggct gaaaagtcca agggataaat gagagaaaac
    11521 ccttggatct atcttgtaaa tttcgaatca catctaaatg ctcgatcact tcagcttttg
    11581 tttcctctga tccaaatacc acattggcac ttcctaaaag cccaacttca tgacaagttt
    11641 ccatcgcgcg aacccattca gtaacgcttg cttttttagg agaaatgata tttctcattc
    11701 tttcagtcaa aatctcagca cctgcacccg gaacagaatc tagtccagca tcttttaaag
    11761 tttttaaaac ttctctcagg ctaaggcctg ttatccgctc taaattaata atttctacag
    11821 gtgaaaaagc cctaatgtgc atttgaggaa aacgttcctt aactgttttt aaaacatcaa
    11881 gataataatc aaattttaat tcaggataaa ctccaccttg taaaaacatt tgatctgcat
    11941 tttcctttgc agcaaattcc attttttcta atatttgctc tttagagaga acataacctt
    12001 ttcctgaacc aatctcatcc ataaaagaac agaaggaaca ttctacattg caatagtttg
    12061 tataatttac tactctaaac atagtataac ttgcatattt atgagataaa aatttttctc
    12121 taattgctct agccacaaat tgtattttta aaaaatcacc ttcttcatag agaagcaatg
    12181 cttcatcact cgtaattcgt tctccatcta gagcttttaa taaaatttta tctgttttgt
    12241 cttctgtttt agatgttaat aacattttca atcctcgatt agatacttta caagaatgaa
    12301 tattcactta tttttgcaat tacaaagaag atttaaaaca aaccaaggaa acatttttga
    12361 aacaatacat acttacctta ttactgtttt taattaccat ccaaataaac agtgatccaa
    12421 ttagaaaata taaaatagtt atcgatccag gccatggagg agtcaaacag gaacccttag
    12481 aaatttttgg tgataaatat gatacgatta cacgcagatt tttggaaaat tacaaagacg
    12541 gcgcaagcta tgcaaaggca aatcgaactg aaatggaaat cgttttagga gtgggaaaag
    12601 agttaaaaga aattttagat ttaaccaaaa caaagaaagg atttcagaaa tttaaatctt
    12661 acgtaaaact tttttctgac tcagaagttc cctggataaa aatagattcc gttatgacaa
    12721 gaacagatag ttataaagat agaaactacc gtgagaagga agacaaaaat caactttata
    12781 gactctacga ttttcccgat tttaagacag gtaaaaatcg tttaggtaga atctcacaga
    12841 ttaacaaaga aaagccacaa ctcgtagttt cattgcatat aaatgcaatg aatagcactc
    12901 cagactctgg tggaatgggc gcagtgattg caccttccta ccaaacattt gaactcttaa
    12961 aaagaatttc agaaaaaaaa gctcaaccag aagaattcct aaaaactcct tggaaaaatt
    13021 ggatgctatt tgaatccaaa tggtcaagac tagaaaatgc aatggctgat gcttggattt
    13081 attttaatgg atattggcca acacaagatg gttcagagac caacaaagaa agattcgaag
    13141 gatatagagc aaatatgatt acttggcgat atcgagatga tatcggttgg gagaagaaga
    13201 taaataaaaa agaaggaccc tacgcgatcg accattctaa ttttagagct gttggtaaat
    13261 tctgggaccg cgaaagaggc aaaccagaat taatgaggag agagggtgga cctgagggtt
    13321 atggcggaga taatctttat gcaagcaatg agctattgcg atttttgcag tatggtctgc
    13381 gtatgcaagt gaatgaagat aaagataatt acaaagaacc aaataaaatt ttattaccct
    13441 ttatatccac ctatagtgtg cctacttata tcaacgcaat atcagcctat ttagaattgg
    13501 gagaaattac aagtgaccgt gacatgtatt ttgttacaac caaaaagcgc aaaacagcaa
    13561 tttgcctcgc agttggaatt tattcattat tcaatggatt agaaattaaa caaatggaaa
    13621 cattatatac tcccaaagga aagcgaattg attttgaaaa atatattgga agcacaggac
    13681 aaagttactt tgatgaagta acaacagaca aaaatgagta aacctacacc actaaaagaa
    13741 aaagaatttg gtctattaat acttgcctat agaaaatctt taataaagcg ttattctaga
    13801 gaaaatctaa agcgctttaa agagtttgaa ggcattgacc agtcaactgt agataaacta
    13861 acgtattatt ttttggaatt attgtatcct gagttagagg aaaggcttaa actagaccaa
    13921 gcctttgatt ccctagcttc attcgtgcac aatccagata aaatgtttgg acttctagga
    13981 agcgttggaa ccattcttta taaattcggg aaacaatttt tctctgcaac aaaagcagga
    14041 atttctgcct taagatctta tgtaactgcg cataaatttg aatcgatact ttatgagaat
    14101 gcaaagaaaa tgattcaaga aggaaaggat attgaagaag atacaaattt taacaaactg
    14161 atagcaagca ttccaagaaa agaagcggat gattttagac atcagatcgt taatcttttc
    14221 tcaactttgt ccagaaaaga attactacaa aagatattag aagttatgga ttatgttatt
    14281 gataaaatga aatcaaagcc agaaacatat tctaaggatg atattgatgg aatatctcta
    14341 ggaagagaaa ttattattca tgggaaagaa gtttttacaa atctttcaca ggaacaaatt
    14401 gatttaattt tgaaagggat tgatacgatt gagaaagatt tttattatag agctttagaa
    14461 accaaatagt ttggtgccga tggtcggaat cgaaccgaca cgaggttgcc ctcggtggat
    14521 tttgaatcca ctgcgtctac caatttcacc acatcggct
//
LOCUS       DAOQOS010000132        14423 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_2148_length_14423_cov_5.273524, whole genome shotgun sequence.
ACCESSION   DAOQOS010000132 DAOQOS010000000
VERSION     DAOQOS010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 14423)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14423)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14423
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2148_length_14423_cov_5.273524"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..572
                     /locus_tag="PLF66_21315"
     CDS             <1..572
                     /locus_tag="PLF66_21315"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HMZ66642.1"
                     /translation="SAVDDLSIPTDAWKKERPRIFFYLRTKTPRRGAYLGLLALRELK
                     RKMPEVEIVIAGEESSHLSLDFEVTNLGLLQLSQLHDIYKSVDLALVLSFTNLSLLPL
                     ELMASNCTLLINDAPNNRWGLNENICAFCDTTVESIVDKITELLKNHRLRNRLRKNAI
                     EYVGTTSWEKEAEKVYKILTKLQKKYLNG"
     gene            565..1500
                     /locus_tag="PLF66_21320"
     CDS             565..1500
                     /locus_tag="PLF66_21320"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HMZ66643.1"
                     /translation="MDNVYILLSTYNGEKFLEEQLKSLKKQTGLFQILVRDDGSSDNT
                     LEILSRWEKELPIKILKGENIGVIQSFQVLLQSVPKDAEYIFFCDQDDIWEENKITKS
                     IEFLKEGERLYGKKIPLLFHSDLSVIDDTSKQINPSLWKSQRIRWKLGQKLNRLVMQN
                     TVTGCSMMINSALLGFITEIPKEAKMHDWWCALVACSFGKIISSEETLIRYRIHGGNV
                     VGARAIDLQRILNVFLNLKNYIHKWRQENQTRTQQVEAFYKMYKTLLPLEKEDILRNY
                     LIASEKGFLIRKLYQIRYQYLQQGWFRVIISFLLF"
     gene            complement(1489..2370)
                     /locus_tag="PLF66_21325"
     CDS             complement(1489..2370)
                     /locus_tag="PLF66_21325"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HMZ66644.1"
                     /translation="MMIGIMVSYEPDSKELVQNINSNLEELDLLILVENGSSPTLQNQ
                     IAKQIQNDRCILLLQDRNLGLGKAQNIGLKKGFELGGEYFLFLDDDSVLKKGSVKILK
                     EELFKNPKLGIAACHVLHEGSKKEQKYWIESGFFYKRVRFTESSHKLEKVNTVISSGS
                     LIPKSVIEKCGFMEEDYFIDYIDIEYCLRVRKNGYTISVYKDAVLFHKLGNTKTISVG
                     NLQFHPTNHSPERRFYMIRNRIWTWRKYGFSFFGWFLIDLGNFFFDNLRVLLFEKDRY
                     LKMKFFWKGLVEGFLKK"
     gene            2633..3352
                     /locus_tag="PLF66_21330"
     CDS             2633..3352
                     /locus_tag="PLF66_21330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66645.1"
                     /translation="MIDSDFLKKIGKEALFSLSTLLILTGVGTFLNFIFLFFLTPELN
                     SLFHTSAGMGHASGLGAILALFLLLIYNWQASLLFVSFFLGFPFLHFLIVKKYVIMKA
                     LFHILQEKKSKMVEYILNQFFERIKNRLEWLDKLNSSGTISVLNEYLPIYIQKLEGMP
                     FMIRWIVKLFLGRFDFLGIVSGVLNEQGQIDIGQKEIINKISDKVNTVLDEKLFSPSF
                     TGIITLYIINISAFIIIKVIL"
     gene            3352..5556
                     /locus_tag="PLF66_21335"
     CDS             3352..5556
                     /locus_tag="PLF66_21335"
                     /inference="COORDINATES: protein motif:HMM:NF013109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HMZ66646.1"
                     /translation="MQRQKINYLNIKEFIGGFSALLVALPAAMAFGLIIFSGLGESYK
                     QQSAVVGIIGTILLSITASFFGKTPGMISAPSAPASAVLSAFVMELVHRKHVSIEYIP
                     IFVTLTALFAGLFQFITGRAGGGKLVKYIPYPVVTGYLGSVGLLIILGQLPKVFGFSK
                     INDVLMSFSSLSTINLVHLMIASATIIAMLVVTRISKKIPPAIFSLVIGGFTFLILGY
                     FDRGLFSLEKNPYVIGSISVTPTQFIDNVFQTWKHIAGFDLDIVLNLIVPVTTLAFVL
                     SIDTLKTCIVHDTVMQTRHDSNQELMGQGIANIVSALAGGIPGSGTLGPTLVNLNSGG
                     RTKLSGALVGIFAMIILFFAPQILAWIPVAALAGVLIVLGFRMIDWKSFRLLRNRYTI
                     IDFFVIIIVIVSALVYDLVTAAGTGIAMAILLYIREQVRSNVVHRKFFGNQKFSKKRR
                     LPSEIEILERRGKATLVLELQGQLFFGTTDQLFTVLEFYLTHSKYIILDMRRVLSIDF
                     TAAHMLDQIHSRYEANGGLLLISSIPLDLPSGQNIQEYLLNLGFSDSKTLKLFPDLEL
                     ALEFVEDEILKTELRGTTVAKNEMKLEDFEFFSGVDKKVISNLASHFKQKKLSSGEYI
                     FKKGDMGDEIYFIRKGDVKIELPISLEQTLIITILARGDFLGEIAFLDANPRSANAVA
                     SGDVSVYYMSRKELDKVFKKYPKLGNVFFERLARAISTRLRLTDIELAASKQ"
     gene            complement(5557..6111)
                     /gene="lptC"
                     /locus_tag="PLF66_21340"
     CDS             complement(5557..6111)
                     /gene="lptC"
                     /locus_tag="PLF66_21340"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HMZ66647.1"
                     /translation="MKKTILISIILSILFQCKKKNSLRIENEKESGSTISLRNFTRDA
                     FNEKGELVWILKAEEAFVYVNDGKSIFYGLHFDQYENGKIKSKLIGDRAEIDQKLKQL
                     KVNGNIDLIAEDSRHLKAEELFYDLEKETLNTDKPVTIIMKGTTINGIGMEADKGLNK
                     VKILKPAGVSSDNPLGPKIRDTSD"
     gene            6264..8768
                     /locus_tag="PLF66_21345"
     CDS             6264..8768
                     /locus_tag="PLF66_21345"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohemerythrin"
                     /protein_id="HMZ66648.1"
                     /translation="MNYLYQIILFFLLCFPVFFCSKAPITKIENGYLDLSKRESFLET
                     GIVALDGEWKFYWNQFLEANEEYPVERLIKVPSSWNYFKIENQEISGIGYATYILNIN
                     LGEYSKESLSLKFLSVSSAYRLYVNHRLIADVGRVGKSYEEEIPDYSPATYDLPQLNS
                     DTLEIKILVSNFHHNRGGIWRQILLGKKEAIQKARTVNLIQDSFLIGSLFIIGIYHLG
                     FFYQSRKNLSPLYFGIFCIVILFRVFLTGEMLFSYLFSDFPFKWQLRLEYIDFEIATI
                     LGILYFSSIFKDYIYLLANRILIILLSVFILATMVLETRTFTKFLILFQSVQFIVISY
                     MLYILVKAFKSKNEEAKLFILGLSIWTLALINDSLFVLGFVQTGLYAPIGFFLFIFLQ
                     AFLLSSLFNKALIGVAELSEKLIQINQSYSRFVPMEFLSYLNKKDVTNVNLGDVIETE
                     VAILFCDIRSFTSLSEKSEPSDLFRFLNSYFDKMNQIIIKHNGIIDKYIGDAILAIFP
                     SNSENAVHCAIEMQETLRDNYFILNGEKRKIQAGTALHYGNVLLGVIGGQKLLQATVI
                     SDAVNTSARLQSLTKTYGCGIIMSESLLLNLDDANIFRIRFLDHALVPGKEDTLFVCE
                     VYDSDPKEQIDLKLETKKAFDTGVSFYHLGEYEKAWDNFIDVLKINPQDSPAIVYMNR
                     AATFLVKGLTILQESEEKLVKWDSKWETGIRIIDEQHRMLFDIINDLHKAMKHSRERE
                     ILERILKNLKMYIFTHFTLEEELMLKVGYDDFANHKKEHSKFIETMNGIASDFEKDQR
                     ESSKEILEFLKDWLIYHIQKSDKEGYVPFVPNYDRN"
     gene            9037..9978
                     /gene="fmt"
                     /locus_tag="PLF66_21350"
     CDS             9037..9978
                     /gene="fmt"
                     /locus_tag="PLF66_21350"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004767774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HMZ66649.1"
                     /translation="MKIGYFGTPEHSAKLLEALILEGHQILFVATNQDKPVGRDKALT
                     PSPVKKKALENNIPVLQFPNIRNDAAIEKINSFDADIYVVFAYGYIIPSRIFNHPKLG
                     TINLHGSLLPEYRGASPVQSAILEGKTVTGVTIQYILEELDAGNIISQKEIQILKEDT
                     FGTLLEKITNVGITEVIQLLKNPTMERFPSKPQNHSDATHCRKIKPEDRKLDFREDSI
                     TLFNKIRAFNPGYICYTTFREKRLNIYSTHILEDHSIEPLKKPGSVHLIGKKTIGVEC
                     GDSKILILESVQPENKKVMSSLDFINGNKPQTGEVFL"
     gene            9975..10973
                     /locus_tag="PLF66_21355"
     CDS             9975..10973
                     /locus_tag="PLF66_21355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020768446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HMZ66650.1"
                     /translation="MKEFLEKLKVVPVLGYLLFITTGLIVFFLAAFLVVLLRTKSAAS
                     VLMPDLVGQQYTEVHNELMRLRLKVRIENKRFPEKNDGEILYQSIGAGKNVEAGSKLY
                     LTVNTGVDRVKMPDLKGQKLNNAKAALDKILSGETYVSLPLGGITYVPVTEGQTPETV
                     ISQIPEPGKTITTREKVFLLVTEPDVKDKSSRVEDFKGFPFPLIAKILNTKKITYKIS
                     EIIPTKDKRENGLIESYETKSDGRVTFKIFYFEPENKVQSGYEKISYKVDHSKPYSLE
                     VQNLEDESKVQKLFENISFQKEEELNFVFYREGDVKVSVVDGDGKKEKSFKFRSDL"
     gene            10973..11620
                     /gene="rpe"
                     /locus_tag="PLF66_21360"
     CDS             10973..11620
                     /gene="rpe"
                     /locus_tag="PLF66_21360"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010419394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HMZ66651.1"
                     /translation="MKISASILAAEISSLGTTIPKLKPKAIDLIHMDIMDGNFVPQIS
                     FGEALTEEVSKLTKIPLDVHLMVKNPELHVPKYYKLKPEFITFHREATDFPIRLAEDI
                     RKNGSKVGIALNPATPVKMIQDCLPYIDLILIMTVEPGFYGQSFVKSGLEKINRAKEL
                     IGSYSIQLEVDGGVNPSNIERLADIGVNICVAGSALFKNGKPNESALSLKKLTNN"
     gene            11688..11945
                     /gene="rpsP"
                     /locus_tag="PLF66_21365"
     CDS             11688..11945
                     /gene="rpsP"
                     /locus_tag="PLF66_21365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HMZ66652.1"
                     /translation="MVKLRLQRTGTKNEPHYRIVAADSRFPRDGRFIEVVGQYHPINK
                     SNQVVINDEKTISWLKKGAQPTNTVKNIFKKSGVWSKFKAK"
     gene            11948..12184
                     /locus_tag="PLF66_21370"
     CDS             11948..12184
                     /locus_tag="PLF66_21370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016757480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HMZ66653.1"
                     /translation="MHSEALLRYVVSFLVDKPDAVNVREVQADSETILELMVAPEDIG
                     KVIGRNGRIAKSLRTLIAASGAKEGKNYSLEIID"
     gene            12177..12698
                     /gene="rimM"
                     /locus_tag="PLF66_21375"
     CDS             12177..12698
                     /gene="rimM"
                     /locus_tag="PLF66_21375"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HMZ66654.1"
                     /translation="MTNDYISIGKIRSTFGLKGHLKVFSDGDILSSLEPHASIYINEN
                     EDFVSYEVETLHGTKPQFILQLKNITLIEQAEKLIGKVVYYPKKKAEAFLEEGELFTH
                     QLIGLVPYFQDKDYSPYILKEVISNPVHPILLFENGSSEILVPYINEFVGEVDLSKGR
                     IEILNWEDWLNAN"
     gene            12688..13350
                     /gene="trmD"
                     /locus_tag="PLF66_21380"
     CDS             12688..13350
                     /gene="trmD"
                     /locus_tag="PLF66_21380"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004460660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HMZ66655.1"
                     /translation="MQINFITLFPQKIQSYFQEGLPAKAIEKGVFQIKIIHLRDFSSD
                     KFGRVDDTVYGGGPGMLLKVEPIHKALLSLGEEKGRVCLMSPSGTPFTQQIANDFANK
                     KENLTFISGYYEGIDHRVTEHLVDIELSLGNYVLSSGDLPSLCVSDSVIRLLPGFMGD
                     SYGSLVDESHNKEGQLEYPQYTKPQNYNNWLVPEVLISGNHLEIAKWKEKNRKHIMNE
                     RK"
     gene            13353..13796
                     /gene="rplS"
                     /locus_tag="PLF66_21385"
     CDS             13353..13796
                     /gene="rplS"
                     /locus_tag="PLF66_21385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008591513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HMZ66656.1"
                     /translation="MNHEIAKLFESDMNRRKINFAVGDTVKVHYKIVESGKERTQVYE
                     GVVISISNKMNDKSFNVRRVSYDVGVERIFPLYSPKISKIELVRKGHVRRSKLYFLRE
                     KSGKTARIKELRGGQAIVQAERKKDRESAQSAKEAGAENQVTAAA"
     gene            13868..>14423
                     /locus_tag="PLF66_21390"
     CDS             13868..>14423
                     /locus_tag="PLF66_21390"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF000595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HMZ66657.1"
                     /translation="MFGVDEAGRGPLAGPLSFAAVKFSKEKLEQILRGNLLPQLNDSK
                     KISEKKREEIYEEIVNSADLVLHQFISNKFIDKYGISISIFDAIRRLVIKSREKSIFL
                     LIDGNYNFLKRQAEKNFQFNYRSIVKGDARIASIAAASIVAKVRRDRYMNRVAEYYPD
                     YQFEKHKGYGTELHIQKIREFGYSS"
BASE COUNT         4826 a         2233 c         2603 g         4761 t
ORIGIN      
        1 actccgctgt agatgatcta agtattccga ctgacgcatg gaaaaaggaa agacctagaa
       61 tcttttttta tttaagaacg aaaactccaa ggcgaggtgc ttatttaggg ctactcgcac
      121 tgagagagtt aaaaagaaaa atgcctgagg tggaaattgt aatcgcggga gaggagagta
      181 gtcatctctc tcttgatttt gaggttacaa atttaggatt gctccaatta tctcaactcc
      241 atgatattta taaatctgtt gatctagctc ttgttctttc ttttacgaat ctatctcttt
      301 tacctttaga attgatggca tcgaattgca ccttactcat caatgatgct ccaaataatc
      361 ggtggggact aaatgaaaat atttgcgctt tttgtgatac tactgttgaa tctattgtgg
      421 ataaaatcac tgaattatta aaaaaccata ggcttagaaa tagactcaga aaaaatgcaa
      481 tcgaatatgt aggcactaca agctgggaaa aagaagcaga aaaggtttat aagattctta
      541 caaagttaca aaagaaatat ttaaatggat aatgtttaca ttttactttc tacttataat
      601 ggagagaaat tcctagaaga acaattaaaa agcttaaaaa aacaaacagg cctatttcaa
      661 attttagtga gagatgacgg ttcttcagat aatactttag agattttatc tagatgggag
      721 aaagaacttc ccataaaaat tctaaaaggt gagaacattg gtgtcattca gagttttcag
      781 gttctattgc aatcagttcc aaaggatgcg gaatatattt tcttttgtga tcaggatgat
      841 atctgggaag aaaataagat tactaaatca atcgaatttc tgaaagaagg ggaaaggctt
      901 tatggaaaaa aaattccact tctttttcat tcagatcttt ctgtgattga tgatacatct
      961 aaacaaataa atccttcctt gtggaagtct caaagaattc gttggaaatt aggacaaaaa
     1021 ttaaatcgtc ttgtaatgca aaataccgtt acagggtgct ctatgatgat caattccgct
     1081 ttattggggt tcattactga aattccaaaa gaagcaaaga tgcatgattg gtggtgtgcg
     1141 ttagttgctt gttcctttgg aaaaattatc agttctgaag aaaccctaat ccgatatcgt
     1201 attcatgggg gcaatgtagt tggagcaagg gcaattgacc tacagagaat tttaaatgta
     1261 tttttaaatt taaaaaacta tatccataaa tggagacaag agaaccaaac tagaactcaa
     1321 caagtggaag ctttctacaa gatgtataaa actttgcttc ctttggagaa agaagatatt
     1381 ctaagaaatt atttaattgc ctcagagaaa ggatttctaa tcagaaagct ttatcaaatt
     1441 agatatcaat atttacagca aggttggttt agagttatca tatccttttt acttttttaa
     1501 aaatccctcc actagacctt tccagaaaaa tttcattttt aaatagcgat ctttctcaaa
     1561 gagtaaaact ctaagattat caaaaaagaa atttcctagg tcgattaaaa accatccgaa
     1621 aaaactaaat ccatattttc gccaagtcca gattcgattt cgaatcatat aaaaccttct
     1681 ttcgggacta tgatttgttg gatgaaattg taaattgcca acggaaatcg ttttggtgtt
     1741 tcctaatttg tgaaataaca cagcgtcttt gtaaacagaa atagtataac cattttttct
     1801 aactcgaagg cagtattcta tatctatata gtctataaaa taatcttctt ccataaaacc
     1861 acatttctca attacagatt ttggaatcag tgaacctgaa gagataacgg tatttacttt
     1921 ttctaatttg tggctactct ctgtaaacct cacccttttg taaaaaaatc ctgattcgat
     1981 ccaatacttt tgttcttttt ttgatccttc atgtaaaaca tgacaggctg cgattccaag
     2041 ctttggattt ttgaataatt cctcttttaa aatttttaca cttccttttt ttaaaacaga
     2101 atcatcatct aaaaataaaa aatattcccc acctagttcg aatccctttt ttaatccaat
     2161 attttgtgct tttcccaacc ctaaatttct atcttgcaga agtagaatgc atctgtcgtt
     2221 ttgaatctgt tttgcaattt gattctgtag agtaggactc gagccatttt ctactaagat
     2281 tagtagatcc aattcttcta aatttgaatt tatgttttga actagttctt tagaatctgg
     2341 ttcatagctt accattattc caatcatcat tttcttttat aacttctttt gtatcacttc
     2401 aaaaagaatt ggagactgct gcaaattaaa gcaattctaa ttctctttcg ggaatattct
     2461 tttatctttt ctttattgaa tcgtatcaaa tcggtttctt tgctgtaaac tgttttaatt
     2521 aaattaaata ggaaaggctt caacaaaaat aaggaattgg aaataagatt tgttttaaat
     2581 ttttacaaaa aaattctctt gcttctattg gattctattc tataaaagaa aaatgattga
     2641 ttcagatttc ctaaagaaaa tagggaaaga ggctttattt tccctttcca cattattgat
     2701 cctaaccggt gttgggacat ttttaaattt tattttttta ttttttctga caccagaatt
     2761 aaactcttta tttcatacgt cagccggtat ggggcatgcg agtggtctag gtgcaatttt
     2821 agctttattc ttactactga tctataattg gcaggcaagt cttctctttg taagcttttt
     2881 tttagggttt cccttcttgc attttctaat cgtaaaaaaa tatgtaatca tgaaagcatt
     2941 gtttcatatt ctccaagaga aaaaatctaa aatggtagag tatatcttaa accaattctt
     3001 tgagagaatc aaaaacagat tagaatggtt agataaatta aattcttctg gaacgatctc
     3061 tgttttaaat gaatacttac caatctatat acagaaatta gaaggaatgc cttttatgat
     3121 tcgatggatt gtgaagttat ttctaggacg gtttgatttt ttgggtattg tatctggcgt
     3181 tctaaatgaa caaggacaaa tcgatatagg tcaaaaagaa attataaata aaatttcaga
     3241 taaagtaaat acagttttag atgaaaaact tttttctcct agttttactg gaatcattac
     3301 tctttatatt attaatattt ctgcttttat aatcataaag gtaattcttt gatgcaaaga
     3361 caaaaaataa actatttaaa cataaaagaa tttatcggtg gattctctgc acttcttgta
     3421 gcacttcctg ctgctatggc atttggtctt attatttttt ctggattagg tgagtcttat
     3481 aaacaacaat cagctgtagt tggaatcatt ggcacaatct tactgagtat aaccgcttct
     3541 ttttttggaa aaacacctgg aatgatttct gctccaagtg ctcctgcaag tgctgtctta
     3601 tctgcctttg ttatggagtt ggttcataga aagcatgtgt ctattgagta tattcccatc
     3661 tttgtgacgt taaccgcttt atttgctgga ttatttcaat ttatcactgg tagagcaggc
     3721 ggtggtaaat tagtaaaata cattccctat cctgttgtga ctggatattt aggaagtgtt
     3781 ggtttgttga ttatacttgg acaacttcct aaagtatttg gttttagtaa aatcaatgat
     3841 gttttaatga gtttctcctc tctctctacg ataaaccttg tgcatttaat gatagcaagc
     3901 gctacaatta ttgcaatgct agtagtcaca agaatttcaa aaaaaattcc tcctgcaatc
     3961 ttctctttag tcattggtgg ttttacgttt ttaattttag gatactttga tcgaggtcta
     4021 ttctctctag agaaaaatcc atatgtgatt ggttctatct cggtgacgcc tacacaattt
     4081 atagataatg tttttcaaac ttggaagcat atagctggat ttgatttgga tatagtatta
     4141 aatttaattg ttccagtcac aacactagcc tttgttttat ctatcgatac attaaagact
     4201 tgtattgtgc atgatactgt gatgcaaaca agacatgatt ccaaccaaga gttgatggga
     4261 caaggaattg ccaatatagt ttctgccctt gcgggtggta ttccaggatc aggaacgtta
     4321 ggaccgacac tcgtgaatct caatagtgga ggcagaacga aattatcagg agctttggtt
     4381 gggatttttg caatgatcat tcttttcttt gctcctcaaa tacttgcttg gattccagtt
     4441 gctgcacttg ctggagtctt gattgttttg ggatttagaa tgattgattg gaagagtttt
     4501 cgtctgcttc ggaatcgtta tactattatt gattttttcg tgattataat tgtaatcgtt
     4561 tctgccttgg tttatgattt ggtgacagca gccggaacag gtattgccat ggctatacta
     4621 ctttatatta gagagcaggt tcgttccaat gtagtgcata ggaaattctt tgggaatcaa
     4681 aaattttcta aaaaacgcag actcccttct gaaatagaaa tcttagagag aaggggaaag
     4741 gcgacgttag ttcttgagct gcaaggacaa cttttcttcg gaacaacaga tcaattattt
     4801 acggttctag aattttacct aacccattcc aagtatataa ttttggatat gcgtagagtt
     4861 ttatcgattg attttacagc cgctcatatg ctcgatcaaa ttcactctag atatgaggct
     4921 aacggtggtt tgcttttgat tagttctatc cctctcgatt taccatccgg tcagaacatt
     4981 caggaatatc tattgaatct tggtttttca gatagtaaaa cattaaagct ttttcctgat
     5041 ttggaacttg ccctcgaatt tgtagaagat gaaattttaa aaaccgaact aagagggact
     5101 accgttgcca aaaatgaaat gaaactagaa gactttgagt ttttctctgg tgtagataaa
     5161 aaagtcattt caaatcttgc ctctcatttt aagcaaaaga aattatccag tggagaatat
     5221 atttttaaga aaggagatat gggagatgaa atctatttta tccgaaaagg ggatgttaag
     5281 attgaacttc ctatttctct agagcaaacc ttgattatca caattttggc tcggggggat
     5341 tttttagggg aaattgcttt tttagatgca aatcctcgat ctgccaatgc agttgcctct
     5401 ggagatgttt ccgtctatta tatgtctaga aaggaactag acaaagtatt taaaaaatat
     5461 ccaaaattag gaaatgtatt tttcgaaaga ctcgctagag ctatatcgac tcgacttcgt
     5521 ttgacagata ttgaattggc tgcttctaaa cagtagctaa tcactagtat ccctaatttt
     5581 gggaccgagt ggattatcac tgctgactcc tgcaggctta aggattttga ctttgtttaa
     5641 tcctttgtct gcttccattc caatcccatt aatagttgtt cctttcatga taatggtaac
     5701 tggtttatct gtgtttaacg tttccttttc cagatcataa aataattctt ccgcttttaa
     5761 atgcctgcta tcttctgcaa ttagatctat atttccattt acttttaatt gttttaattt
     5821 ctgatctatt tctgccctgt caccgattag tttggatttg atttttccat tctcatattg
     5881 gtcaaagtga agcccataga aaatagattt tccatcattt acataaacga aagcttcttc
     5941 cgcttttaag atccagacta gttctccctt ttcattaaat gcatccctag taaaattacg
     6001 taaactaatg gtagaacctg attctttttc gttttctatt ctcaggctat tttttttctt
     6061 acactgaaat aaaatgctga gtattatgga aataaggata gtttttttca tacaacccca
     6121 tgatctaatt ctaaattgga attacaatct attttaaatc cacttagaat ttaagctttt
     6181 aactagaaaa aatgttgacc ctgattagat tcaatagctt attaagcgaa ggttattttt
     6241 caaatttgcc aaccgaaaat aaaatgaatt acctctacca aatcattctt ttttttctac
     6301 tctgttttcc tgttttcttt tgcagtaaag caccgattac aaagatagag aatggatatc
     6361 ttgatttgag taaaagagaa tcgtttctag agactggaat cgttgcactc gatggagaat
     6421 ggaagtttta ttggaatcaa tttctagagg caaatgaaga gtatccagtc gaaagattga
     6481 taaaagttcc ctcctcatgg aattatttta aaatagagaa tcaggaaatt tcaggcatcg
     6541 ggtatgcaac ttatattcta aatataaatt tgggagagta ttccaaagaa tcgcttagtt
     6601 taaagttttt aagtgtttcc tccgcttacc gtttatatgt gaatcataga ctcattgcag
     6661 atgtgggtag ggttggaaaa tcatacgaag aagaaattcc tgactattct ccggctactt
     6721 atgatttacc gcaattgaat tcggatacgc tcgagatcaa gattttagtt tctaattttc
     6781 atcacaaccg aggtggaatc tggaggcaaa ttcttttagg taagaaagaa gccattcaaa
     6841 aagcaagaac tgtaaactta attcaggatt cctttctgat cggtagcttg tttataatcg
     6901 gaatctatca tttgggtttt ttctatcaat ctagaaaaaa tttatcgccc ttgtattttg
     6961 gaatcttttg tattgtgatc ttatttcgcg tttttttgac aggagaaatg ctttttagtt
     7021 atttgttttc ggactttcca ttcaaatggc aacttcgctt agaatacatt gactttgaaa
     7081 ttgcaaccat cctaggtatt ctttatttta gttctatatt taaagattat atttatcttt
     7141 tagcgaatcg aatcctcatc atcttattga gtgtttttat tcttgctacc atggttctag
     7201 agactagaac ttttactaag tttttgatcc tttttcaatc tgttcaattc attgtcatta
     7261 gttacatgct ctatatttta gtgaaagctt ttaaatccaa aaatgaggaa gcaaaacttt
     7321 ttatattagg actttccatt tggactcttg cgctcataaa tgatagttta tttgttttag
     7381 gttttgttca aacaggtctt tatgcgccta tcggtttctt tttatttata tttttacaag
     7441 catttttact ttcttcttta tttaataaag cactcattgg agttgcagaa ttatctgaaa
     7501 aattaattca aatcaatcaa tcctatagta gatttgtgcc aatggagttt ctatcttatc
     7561 taaataaaaa agatgtaaca aatgtaaact taggcgacgt gattgaaacg gaagtagcta
     7621 ttcttttttg tgatattcga tcttttactt ccttatctga aaaatcagaa ccatctgatt
     7681 tatttcgttt tttaaattcc tattttgata agatgaacca aattataatt aaacacaatg
     7741 gaattataga taaatatatt ggggatgcaa ttcttgcaat ctttccaagt aattcagaaa
     7801 atgcagttca ctgtgcaatc gagatgcaag aaactcttag ggataattat tttattttga
     7861 atggagaaaa gagaaaaatc caagcaggga ctgctttgca ctatggaaat gttctactgg
     7921 gtgtgattgg tggacaaaaa ttattacagg ctacagtcat atcagatgca gtaaatactt
     7981 ctgctcgatt gcagtctctt actaaaacct atggatgcgg cattatcatg agtgagtccc
     8041 ttcttctgaa cttagatgat gcaaatattt ttcgaattcg attcttggat catgcgttag
     8101 ttcctggtaa agaagataca ctctttgttt gtgaagtgta tgattcagat cccaaagaac
     8161 aaatcgatct aaaactagaa acaaaaaaag catttgatac aggtgtatct ttttatcatt
     8221 taggcgaata tgaaaaagct tgggataatt ttattgatgt attaaaaata aatccacaag
     8281 attcacctgc aattgtatat atgaatcgag ccgcgacctt cttagtaaaa ggactaacga
     8341 ttttacaaga gtcagaggaa aaattagtga aatgggattc taaatgggaa accggaattc
     8401 gtattattga tgagcaacat cgcatgctat ttgatataat caatgattta cataaggcta
     8461 tgaagcatag tagagagaga gaaatattgg aacgcattct aaagaattta aagatgtata
     8521 tttttaccca ctttacattg gaagaagagt tgatgttaaa agtaggttat gatgatttcg
     8581 cgaatcataa aaaagagcat tctaaattca ttgaaactat gaatgggata gcttctgatt
     8641 ttgaaaagga ccaaagagaa agttcaaaag aaattttaga attcctaaaa gactggctta
     8701 tctatcatat acaaaaatca gataaagaag gctatgttcc ctttgttccg aattatgata
     8761 gaaattgatt tgtaatcttt ataagccaaa taaattaggt taatcattct gtaaaaaatt
     8821 tagaatcaat caattgaata aaaaaactca atcattgatt ttgttaagca atgataatgt
     8881 aagttgagtt attaaataac tcatccagta tttgaagaat taatcaaaaa ttttgaaaaa
     8941 ggaatattag ttagatagct atgaatcaaa tgattgcaat taaaaatgat tatttggtta
     9001 ctatcgttaa atcaaatcag agagaaagag gattagatga aaattggata ttttggaaca
     9061 ccggaacatt ccgctaaatt attagaagca ctcattctag aaggtcatca gatcttattt
     9121 gttgcaacca atcaagacaa gccggttggg cgagataaag cattaacacc ttctcctgta
     9181 aaaaagaaag cattagaaaa caatattcct gtattacaat ttcctaatat acgaaatgat
     9241 gcagcgattg aaaaaattaa ctcatttgat gcagacatct atgttgtatt tgcctatggg
     9301 tatattattc catctcgaat ttttaatcac ccaaaattgg gaacgatcaa tttacatggg
     9361 agtcttcttc ccgaatatag aggtgcttct ccggttcagt ctgcaatttt ggagggaaaa
     9421 actgtaactg gtgtaacgat acaatacatt ttagaggagt tagatgcagg aaatattatt
     9481 tcccaaaaag aaatccaaat tttaaaagag gatacctttg gaactcttct agaaaagata
     9541 actaacgtcg gtattacgga agtaattcag ttgttaaaaa atcctacaat ggaacgtttt
     9601 ccctcaaaac cgcaaaatca tagcgatgct actcattgta gaaaaattaa gcctgaagat
     9661 agaaagttag attttaggga agattccatt accttgttta ataaaatcag agcttttaat
     9721 ccaggttata tttgttatac aacctttaga gaaaaaagac tcaatattta ctctacacat
     9781 attttagagg atcattcgat tgaacctttg aagaaaccag gttcggttca tttgattggg
     9841 aaaaaaacga ttggggtaga gtgtggggat tctaaaattt taattcttga atccgttcag
     9901 ccagaaaata aaaaggtaat gtcctcattg gattttataa atggcaacaa gccacaaaca
     9961 ggagaagttt tcctttgaaa gaatttttag aaaaactaaa agtggtccct gtattgggat
    10021 atttattatt tattacaaca ggattaatcg tattttttct agcagcattt ttagttgtat
    10081 tattaagaac caagagcgca gcgagtgttc ttatgccaga tctagttggg caacagtata
    10141 cagaagtgca caatgaattg atgcgacttc ggttaaaggt tcgaattgaa aacaaaaggt
    10201 ttcccgaaaa gaatgacggg gagatattat accaatccat aggagcaggg aaaaacgtag
    10261 aggctggctc caagctttat ctaactgtaa atactggggt agatagagtt aaaatgcctg
    10321 atttaaaagg gcaaaagcta aacaatgcaa aagcagcctt agacaagatt ctatccggag
    10381 aaacatacgt tagtctacca ctcggtggga tcacatatgt gcccgtaacc gaagggcaaa
    10441 caccagaaac tgttatctct caaattccag aacctggaaa gacaattaca actagagaaa
    10501 aagttttttt acttgtaaca gagccagatg taaaagataa atcatcccga gtcgaagatt
    10561 tcaaaggatt tccgtttcct ctgatcgcaa agatattaaa taccaaaaaa ataacgtata
    10621 agatatcaga aataattcct acaaaagaca aacgcgaaaa tggtctgatc gaatcctatg
    10681 aaacaaaatc agatggacga gtgacattta aaatctttta ttttgaacca gaaaataaag
    10741 tccagagtgg atacgaaaaa atatcctata aagtggatca ttctaaaccc tatagcttag
    10801 aagtccaaaa tttggaagat gaatcgaaag ttcaaaaact ctttgagaat atttccttcc
    10861 aaaaagagga agaactcaac tttgttttct atagagaggg agatgtgaag gtctccgttg
    10921 tagatgggga tggcaaaaaa gaaaaatctt tcaaatttag gagtgattta taatgaaaat
    10981 ttctgcatcg atacttgcag cggaaattag ttctctagga actacaattc caaagttaaa
    11041 gccaaaggct atagacttaa tacatatgga tattatggat gggaactttg tcccacagat
    11101 tagttttgga gaagctttaa cagaagaagt aagcaaactg actaagatac cgttagatgt
    11161 tcatcttatg gtaaaaaatc cagagttgca tgtcccaaag tattacaaat taaagccgga
    11221 gtttatcact tttcatagag aggctaccga ttttccgatt cgtctagcgg aagatattcg
    11281 aaagaatgga agtaaagtag ggattgcttt aaatcctgct actcctgtga aaatgattca
    11341 ggattgtctt ccttatattg atctaatttt gattatgaca gtagagcctg gattttatgg
    11401 acaaagcttt gtcaaaagtg gtctagaaaa aatcaaccga gcaaaagaac taatcggatc
    11461 ctattcaatc caattggagg tagatggggg agtcaatcca agcaatattg agagattggc
    11521 agacattgga gtcaatattt gtgtagcagg atcggcgctt tttaaaaatg gaaagccaaa
    11581 tgagagcgca cttagtctta aaaagttgac aaataactag gttttttttt tcttacttat
    11641 gaacgatgat ttccgtattt tgaagcggaa acagaaagga atataaattg gtaaaacttc
    11701 gattacaaag aactggaaca aaaaacgaac cacattatag aatagtagct gctgatagta
    11761 gatttccaag agatgggaga tttattgaag tagtaggaca atatcaccca atcaacaaat
    11821 ctaaccaagt tgtgatcaac gatgaaaaaa caattagttg gttaaaaaaa ggtgcacaac
    11881 caaccaatac tgttaaaaat atttttaaaa agtctggtgt ttggagcaaa ttcaaagcta
    11941 aataattatg cattcagaag cattacttag atacgttgtt tcctttttag ttgataagcc
    12001 tgacgcagta aacgttagag aagtgcaagc ggacagtgaa accattttag agcttatggt
    12061 tgcccctgag gatatcggga aagtaatcgg tcgaaatggt cgtatagcta aatctcttag
    12121 aactttgatc gctgcttctg gcgcgaaaga aggaaaaaat tacagtttag aaattattga
    12181 ctaatgatta catttcgatt ggcaaaatta gatctacttt tggtctaaaa ggtcatttaa
    12241 aagtttttag cgacggtgat attctttctt cacttgagcc ccacgcttca atttatataa
    12301 acgagaatga ggattttgtt tcttatgaag tggaaacact acatggaaca aagcctcagt
    12361 ttattctgca attgaagaat ataactctga ttgaacaagc cgaaaaatta attgggaaag
    12421 tggtttatta tcccaaaaag aaagctgagg cttttttaga agagggagaa ctgttcacgc
    12481 accaattgat tggtttagtt ccctattttc aggacaaaga ttactctcca tatattttaa
    12541 aagaagtcat ttcaaatcca gtgcatccta tcttgttatt tgaaaatgga agttcggaaa
    12601 ttctagttcc ctatatcaat gagtttgtag gagaggtaga tctttcaaaa ggtagaatcg
    12661 aaattttaaa ttgggaagat tggttaaatg caaattaatt tcattacttt atttccccaa
    12721 aaaattcaga gctattttca agaaggactt cctgctaaag caattgagaa gggagtgttc
    12781 caaattaaaa tcatccatct tcgcgatttc tcttcagata aatttggaag agtagatgat
    12841 acagtgtatg gaggcggacc tggtatgctt ctaaaagtgg aaccaataca caaagcgctt
    12901 ctttctttgg gagaggagaa gggaagggtt tgtttaatga gtccgagtgg aacacctttt
    12961 acgcaacaaa tagcaaatga ttttgcaaat aaaaaagaaa atcttacctt tatttctgga
    13021 tattatgaag gaattgatca tcgagtaact gaacatttag ttgacataga actgagcctt
    13081 ggaaactatg ttttatcatc gggtgattta ccttcccttt gtgtttctga ctcagtgatt
    13141 cggcttttgc caggatttat gggagattcc tatggaagtt tagtggatga gtcccacaac
    13201 aaagaaggac agctagaata tccccagtat acgaaaccgc aaaactataa taattggtta
    13261 gttccggaag tcctaatcag cggaaaccat ttggaaattg ctaaatggaa agaaaaaaat
    13321 agaaaacata ttatgaatga aaggaaataa acatgaacca cgaaatagca aaattatttg
    13381 aatcagatat gaacagaaga aagatcaact tcgctgtagg agatacagtg aaagttcatt
    13441 ataaaattgt tgagtctggc aaagaaagaa cacaggtata cgaaggagtt gtgatttcaa
    13501 tttctaacaa aatgaatgac aagtctttca atgtgagaag agtttcttac gatgtcggag
    13561 tggagagaat ttttcctctt tattcaccaa aaatttctaa aattgaactc gtcagaaaag
    13621 gtcatgtaag aagatctaag ctttatttct tacgtgaaaa gtctggtaag actgctcgta
    13681 ttaaagagtt acgtggcgga caagcgatcg ttcaagcaga aagaaagaaa gacagagaat
    13741 ctgctcaaag tgcaaaagaa gcaggagcag aaaaccaagt aacggctgca gcatagaact
    13801 atttagtcca tcccactctg gatttagatt tgaaccggaa gaattaaacc ttccggagaa
    13861 atattctatc ttcggagtag atgaagcggg tagaggacct ttagcgggac ctctatcttt
    13921 tgcagcagta aaattctcca aagagaaact agaacaaatc ttaagaggta atttactacc
    13981 tcaactcaac gattctaaaa aaatttccga aaaaaaaaga gaagagatct acgaagaaat
    14041 tgtaaattct gctgatcttg ttttgcatca atttatcagt aataaattta ttgacaaata
    14101 tggaatttca atttctattt ttgatgcaat tcgtcgtctt gtgattaagt cgagagagaa
    14161 gtcaattttt ttattaattg atggaaatta taatttctta aagcgccaag ccgaaaagaa
    14221 ttttcagttc aactatagat ccatcgttaa aggagatgcg agaattgctt ctatcgcagc
    14281 tgcctccatc gtggcaaagg tgagaagaga taggtatatg aatcgagtcg cagaatatta
    14341 tcctgattat caattcgaga agcacaaagg atacggaact gaattgcata tacagaagat
    14401 tcgggaattt ggctactcat cct
//
LOCUS       DAOQOS010000133        14033 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_2237_length_14033_cov_6.830519, whole genome shotgun sequence.
ACCESSION   DAOQOS010000133 DAOQOS010000000
VERSION     DAOQOS010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 14033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14033
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2237_length_14033_cov_6.830519"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..1732)
                     /locus_tag="PLF66_21395"
     CDS             complement(2..1732)
                     /locus_tag="PLF66_21395"
                     /inference="COORDINATES: protein motif:HMM:NF039312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HMZ66658.1"
                     /translation="MKKRIQFLVWLACIGLLTFSFYQTRIVDFDKLERIFALIQSIRE
                     ADATLDQDILKSRYYLIKSYDPLNLSILQIQTNINELKTGALAISKIAPSNKAASEAL
                     DKYVEVFQQRKILIDKFKSQNAILKNSLYYLPSSAGKAKKLSSGGYSTLVDEIVRETL
                     FYTNSGDELNKTQAEATILKLKELNALAINQQKIETEIFIRHCENILSRKKDLDTNVT
                     DIINLPARDSINVFYLSVTDFYNQFLNKVNMYRLVLYNLSIGMLLYIALVIIKLKLAT
                     LAMQNLNQSLVKLNTAFGRFVPHDFLNILNKKNIVEIELGDHVKEEMTVLFSDIRGFT
                     TLSESMSPEDNFKFINSYLGTMGPIVRNNKGFIDKFIGDAIMALFKGESENAIDASIE
                     MLEGLARYNQEHRSGREQVEIGIGLHRGEMMLGTIGENNRMEGTVISDTVNLASRIEG
                     MTKLYGIPLLISDNVFNSIKNKSKYYIRKIDTVKVKGKDNPVTVYEVYNTDSNEVRSL
                     KRKLKPNFERAIHEYYAQNYTEAQKLFNAHLREFPADKAAQIHLDRCNSAILNPTNSL
                     NGGASVLLSK"
     gene            complement(1729..2847)
                     /locus_tag="PLF66_21400"
     CDS             complement(1729..2847)
                     /locus_tag="PLF66_21400"
                     /inference="COORDINATES: protein motif:HMM:NF015131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c peroxidase"
                     /protein_id="HMZ66659.1"
                     /translation="MISIKSPIRGGYPLNRVRQFIDNFFRKASDNDFQGISRALNFIF
                     IVVFSLVTGLIFYFFWPKETNISKLNHSASSNFSVEPIQPIPMELELNLTKVELGRKL
                     FHDPLLSKDNTIACSGCHDLAKGGVDGLPKSIGIDKQEGEINAPTVFNSGFNFRQFWD
                     GRAETLEDQIAGPISNSKEMNNNWEDVIQKIKKKTEYNQQFLMIYPNGVTAENIKNAI
                     AEFERSLYTPNSRFDQYLRGDANAINEEEKNGYKLFKENGCIACHQGVNVGGNMYQTF
                     GVYGNYFEERGSLTNADNGLFNITGQEQDRYKFKVPSLRNVALTAPYFHDGSVDSLEK
                     AIVIMGKFQLGRTLEDLEVKQISAFLKTLTGEYKGKSL"
     gene            3287..6874
                     /locus_tag="PLF66_21405"
     CDS             3287..6874
                     /locus_tag="PLF66_21405"
                     /inference="COORDINATES: protein motif:HMM:NF014427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HMZ66660.1"
                     /translation="MKKTIYLIILALLFTAGCSKKEKKYIPFSMNNASNAPTGNASTN
                     VTGATLSQIRVTPADQSIARNTHANYRATGVYSDGTIKDITEDCTWSVKSETDKASEV
                     SSKKGRFLGTGATTSGSPAKVNASLGSLSGDANLNITSASLSSIQISSVPSLIPGGQA
                     QYIATGIFSDGTTQDISSLVNWSSSNSSNVSIDVSTGAGVGGSTAGTATITATLISPS
                     TFPGVANGGSATTTTQLKNVTIVSITVTGLSEVGAGTTANYQASAIYSDGTSSDITSV
                     ATWSVDSATNAAVSDSSGSKGVLTTISNGSVNVQASYVGVTGTKLVTISSITISSISV
                     SPSSKTIIKGTTTQFTATANYSNGTSADISNTAVWTSSNTSIAGVNTSTSNGGLAQGI
                     NTGSVTITVASGGKTATASLSVSAATLVSISINTNASIAKGTTRQYTATGTYSDGTVL
                     DISSLVTWSSSSGSVVAISNTSGTKGLATGSSQGSSTIQASLNGVNSNTSTLTVTAET
                     LTSISIGSNASVAAGGTKQYSATGTFSDATTQDITSLVTWTSSSAANATISNASSDKG
                     KAYGLAAGTTNITASLPNGTNGITTSGGTIVSNTSVLTVTAASNGSTASVSAVTVGAY
                     DVTIPSGINTTTTPTGNFSGLTYNTANNAAGFIAQIPDYSSPTFTCQAQGTAILGKIL
                     ADSPDLVSSYSQNSVNTVNFTGGCTITYNLSITTSSSKKATELSNHLISNVGILAVGG
                     GSVTSLPASQSGETATTAFRAIVQVTYKTGTGGQTIGIGVTNSADYAANQALLESFLG
                     GTNIAVTGTTYLTKTETKTGKAMPKVDFVWVIDNSASTSTIQTSISGAATTFFNKLGG
                     KNLDYRLGVIATGGDGTDATRTDKCTNPVTPGEQLRSNGWMTSSNTAAEFSGSGGTGV
                     GSKGCGTESGIYYAAKSLGYPNSITYTTTPTVTPRSGSSWGSDYKLVFVIVSDEGDQF
                     RCYNGTYSAGSNSSGTPCSGATGNTDFDNNNNVFVNNGYKVHTITGLDSSGNPGSCSV
                     SGGPSTSQGNVTWPGYKNLSTATGGVAASVCSTDYNAIMDTIVNAVAGDSGYILAKNP
                     VSSSITVKVNGSAVAQDSTNGWQYVPATNSIIFSGTAWPATNASIEISYLYSSDQGAF
                     LKMDSNSSLMAYISQTAKSSAARGTAATIALIVGAILAGRLWRRRNSNK"
     gene            complement(7330..7827)
                     /locus_tag="PLF66_21410"
     CDS             complement(7330..7827)
                     /locus_tag="PLF66_21410"
                     /inference="COORDINATES: protein motif:HMM:NF016955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HMZ66661.1"
                     /translation="MSQSQSNQNNPSKGAVGNSTGFNDYIRKVQKELSDDYLISVYIS
                     LYLRALRSDGVLNYEEKVWFADRVKELYDIDIFKPIQGRYTREEFMEHARQAASAPLD
                     LNKIAAYFEKEKKQELCLELACFAVYTGDREVDPNEEKFLDTLTEVLKISESKKNEIK
                     DRFKN"
     gene            complement(7881..8609)
                     /locus_tag="PLF66_21415"
     CDS             complement(7881..8609)
                     /locus_tag="PLF66_21415"
                     /inference="COORDINATES: protein motif:HMM:TIGR00530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HMZ66662.1"
                     /translation="MNKFFSFLFWGFVAITSILLFFVAFTLWVFSLIFDRKRKWLLHQ
                     FTCFWASLYTWFNPLWNIRIFGKENIDSKKAYVCVSNHQSLLDILIMFRLFSHFKWVS
                     KIENFKVPFIGWNMRFNDYIELDRGKVKSNAKMIKDCIRAISNQNSVMIFPEGTRSLD
                     GTLKAFKLGAFEIAKATKAPILPIVLKGTAHALPKGGIELQGKHDMSITVLPEISPSV
                     FENMSTEELTELVRSKIQEDLLAK"
     gene            complement(8868..9161)
                     /locus_tag="PLF66_21420"
     CDS             complement(8868..9161)
                     /locus_tag="PLF66_21420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66663.1"
                     /translation="MKKILLNLLIILLFACNDNSIQVESPSSNQPIRDLIDNNETNSN
                     SNIYRQQNSISGNNGGSSSVGTSSAGAVGTPPATPAVGTSTGGYAGYPTTSGY"
     gene            complement(9177..10088)
                     /locus_tag="PLF66_21425"
     CDS             complement(9177..10088)
                     /locus_tag="PLF66_21425"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HMZ66664.1"
                     /translation="MRFLFILCFVISYQLYAQVGFTQSGKASFYNDRFHGAKTNSGEK
                     YDMNAFTAAHRELPLNTKIKVTNLKNNKSVIVRINDRGPFHHVRILDVSKAAAKELDM
                     ISQGVANVTLEVVELPNEPVKDTKSIATPTSPAKPNKTSTADTVVLPAKTEAKHESKE
                     EETPSKTTHPEPLPAKSIAANANENVKPEPEKPKVDLKEASSEIFKAGYIYNIHGMIQ
                     NPKGYGIQVGSFSHIFNSLRFCKYMTDMGFSKIFIKADKEPNGTAYRVLIGDYASEED
                     ANLDTAKLREKNRDYLIKKYPENKDLP"
     gene            complement(10103..10549)
                     /locus_tag="PLF66_21430"
     CDS             complement(10103..10549)
                     /locus_tag="PLF66_21430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66665.1"
                     /translation="MTVPKKRKKLDNSIDPVIVREEQDNVNLEANQVEEKVAKPKKAA
                     NTHKSPKAKVEKAFEVIDKIDKNIQEKAIELKKVNALTVQGNEGIHIRFKVDLIRWQD
                     FVDGGKGRTEDDSVVIDFTLPYIWNLPVIGDATKALIKELVKLKIL"
     gene            complement(10536..11267)
                     /locus_tag="PLF66_21435"
     CDS             complement(10536..11267)
                     /locus_tag="PLF66_21435"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HMZ66666.1"
                     /translation="MIISFVQTKGGTGKSTLAVNTTFSNAFQSKFQSIALVELDPQGT
                     LKNWWQERTEENRPSEKVSFHHISSTQKEVIQDGVKNIATHNELLVLDIPGESTSKLH
                     TKFACAISDIVIIPMRTSTNDESAFADNLLPIIKEIIRSYPIKKGNFFILPTFTNAQS
                     NREKIIEYFKDILPDYIGCLDVVYPFRSIYENFNREGMTLLEYVETIKNNKRLYVGGA
                     RAIEDVEGIAHSILQKLAEKTHDGA"
     gene            11909..13846
                     /locus_tag="PLF66_21440"
     CDS             11909..13846
                     /locus_tag="PLF66_21440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010572362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HMZ66667.1"
                     /translation="MNNKISPKKNTLFEFMKDARTKNANIVAQMFRTGSPSLQSITFQ
                     EVYEKIQLIAFGLIKLGTKKGDKVGLVADSGHRFMWLAMGITNIGAVDVPRGTDATSE
                     DLLYIFNHASCKIIILENVKAYEKIQKSLSKLSDLKYIIFYSDPGKIKTPSKVEVLTL
                     DALMELGEKYRKTKPDAFEKMGEAIDPKDLATIIYTSGTTGTPKGVMLSHNNYVWMST
                     KLEEALRATGLELQGDETTLGYLPPWHIGDRLFESTCLGIGVTMAFTNIANLASDLKT
                     VNPTLMFSVPRVWESFYNKILENVKNAPPLRKALFNLASSAAMNYTHNRDYLRGSALI
                     IEPESFTKTLINKTKSLFLTLLLFIPYQLSKVILSKVKAALGNRIRFAFSGAGALPYH
                     IDRFFYSIGLPILETYGMSETTGVSCMRDVARPVIGTLGNNLSEIGLKLIDEKGNEIT
                     KPNVKGVAYHKGQHIMMGYYKDPEKTKAVLSPDGWMNSGDILIRTASGQLKFAGRAKD
                     TIVLLGGENLEPEPIEFALVQSEFIHQAMVVGQDKKTLGALIVPAFDKVEKHFKSQNI
                     ALPANREDWKTDQKILNLFKNEIKTLVSNEKGFKAFEKVTGFAIITKEFEAGKELTQT
                     LKLRRNVMFEMYAKEIEGIYK"
BASE COUNT         4635 a         2578 c         2260 g         4560 t
ORIGIN      
        1 attatttcga taataaaaca ctagccccac catttaacga attcgtagga tttaagattg
       61 cagaattgca tcgatctaaa tggatttgtg cagctttatc agcagggaac tctcttaaat
      121 gtgcattaaa aagtttctgg gcttctgtat aattttgtgc ataatattca tgtattgctc
      181 tttcaaaatt tggttttaac tttctcttca aagaacgaac ttcatttgaa tccgtattat
      241 atacttcata tactgttaca ggattgtctt tgccttttac ttttactgta tcaatttttc
      301 tgatgtaata tttgctttta tttttgatag agttaaatac gttatccgat ataagaagcg
      361 gtataccata aagcttagtc attccctcta ttctggaagc tagattaact gtatcactga
      421 taacagttcc ctccattcta ttattctctc cgattgtgcc caacatcatt tcaccacgat
      481 gtaatccaat ccctatttct acctgctctc ttccacttct gtgctcttgg ttataacgag
      541 caagaccttc taacatttct atggaagcat caatcgcatt ctccgattca cctttgaata
      601 atgccataat agcatctcca ataaacttat ctataaatcc tttattattt ctaacaatcg
      661 gacccattgt tccaagatag gaatttataa acttaaaatt atcctctgga gacatacttt
      721 cagaaagagt tgtaaatccg cgaatatcag aaaataaaac tgtcatctct tctttaacat
      781 gatctcctaa ttcaatttca acgatatttt ttttgttcaa aatatttaga aaatcatgag
      841 ggacaaatct accaaaagct gtattgagtt tgactaaact ttgattcaaa ttttgcatag
      901 caagagtggc aagtttcagc ttaattatta caagagctat ataaagtaac attccaattg
      961 ataaattata tagaactaat cgatacatat tcactttatt taaaaactga ttataaaaat
     1021 ctgtaacaga tagatagaat acatttattg aatctctagc aggtaaattg attatatcag
     1081 taacgtttgt atccaaatct ttctttctgg ataaaatatt ttcgcaatgc cgaataaaaa
     1141 tttctgtttc aatcttttgt tgatttatcg ccaatgcatt taattctttt aatttcaaga
     1201 ttgttgcttc cgcctgtgtt ttatttaact catctcctga atttgtataa aataaagttt
     1261 cacgcacaat ttcgtcgact aatgttgagt atcctccact agaaagcttc ttagcttttc
     1321 cagcagaact tggtaaataa taaagggaat tttttaaaat agcattttga gatttaaatt
     1381 tatcaattaa aatctttctc tgttgaaata cttcaacata tttatcaagt gcttctgagg
     1441 cagccttatt tgatggagca atttttgaaa ttgctaatgc acctgttttt agttcattta
     1501 tattagtttg tatttgcaaa atggatagat ttaaaggatc ataagattta ataagatagt
     1561 atctactttt caaaatatct tgatccagag tcgcatctgc ctctctgatg gattgaatta
     1621 aagcaaaaat tctttctaat ttatcaaaat ctacaattct agtttgataa aatgaaaacg
     1681 ttaatagacc tatacaagct aaccaaacaa gaaattgaat tctctttttc atagactttt
     1741 tcctttatat tcaccagtaa gtgtttttaa gaatgcagat atttgcttaa cttctaagtc
     1801 ctctaatgtc ctacccagtt ggaattttcc cataattaca attgcctttt ctagagaatc
     1861 cacagaacca tcatggaaat agggtgcagt gagggctaca ttccttaaag atggaacttt
     1921 aaatttatac ctatcttgct cttgacctgt tatattaaat aatccattat ctgcatttgt
     1981 aagggaacct ctttcctcga aataattccc atatacccca aatgtttgat acatattacc
     2041 accaacgttt acaccttgat gacaggcaat acaaccattc tccttaaaca atttgtatcc
     2101 atttttctct tcctcattta ttgcatttgc atccccacgc aagtattgat caaaacgaga
     2161 atttggagtg taaagagatc tttcaaactc agcgattgca ttttttatat tttctgctgt
     2221 aactccatta ggatatatca tcaaaaattg ttgattgtat tcagttttct ttttaatttt
     2281 ttgaatcaca tcctcccaat tattattcat ctctttacta ttcgatatgg gtcccgcaat
     2341 ttgatcttct aacgtttctg ctcttccatc ccaaaactgt ctaaaattaa atcctgaatt
     2401 gaaaacggta ggcgcattaa tttctccttc ttgcttatca atgcctattg atttaggaag
     2461 accatctaca ccgccttttg ctaaatcatg acaaccagaa caagcaatcg tattgtcctt
     2521 cgaaagaaga ggatcatgaa aaagttttct tcccaattct acttttgtta aatttaattc
     2581 taactccatt ggaattggtt gaattggctc aacagaaaaa tttgaagatg ctgaatgatt
     2641 taactttgaa atgtttgttt ccttaggcca aaaaaaataa aaaatgagcc ctgtgaccaa
     2701 actaaataca acaataaaaa taaaatttaa agctctactg atcccctgaa aatcattatc
     2761 agaagccttt cgaaagaaat tatctataaa ctgacgcact cgattcaatg ggtagccacc
     2821 tcggattgga gactttattg atatcattca tctagtaaac tttatttttt tttccatgaa
     2881 aaatattcag aaaataatct gcaaaaagct atgaattata attaaaaaaa ggtaaggaaa
     2941 agtctggagt tcgaaactat gagccaagat atattctaaa tgtttagttt tagattacat
     3001 aaatctaaaa ctaactgaca taatttcttt attaaataaa gaatacgcat tgcatttttt
     3061 aaattcatcg ctcagacttc actcttctaa aattaaatag ctagaatctt ataagaagaa
     3121 ctaggtaaaa cttgtttaaa taaatttaca tagatttccc tttttttttc taattcattt
     3181 tgcaaatttt cttttcaaat tttaaaaaaa cttataattg cctacgcaag aatcctatta
     3241 aaatacccgt ctgtatttta tagataagtt tggagataag agagctatga aaaagacaat
     3301 ctatttaatc atattagctt tgctgtttac agcaggctgc tcgaaaaagg aaaagaaata
     3361 cattcccttt tcgatgaaca acgcatcaaa cgcaccaaca ggtaacgcgt caactaacgt
     3421 tacaggtgca actttatcac aaatcagagt aacaccagcg gatcagtcaa ttgcaagaaa
     3481 tactcatgca aattacagag cgactggagt atattctgat ggaacgatta aagatataac
     3541 ggaagactgc acatggagtg ttaagtctga aacagataaa gcatctgaag tttcttctaa
     3601 aaaaggaaga ttcctcggaa ctggcgcaac aacaagtgga agccctgcta aagtaaatgc
     3661 atcactagga tcattatctg gggacgcaaa tttaaacatc acctcagctt cgctatcctc
     3721 tattcaaatt tcaagcgttc caagtttaat tcctggtgga caagcacaat acatcgcaac
     3781 tggaatattt tctgatggaa caacacaaga tatttcttct ttagtaaatt ggtctagtag
     3841 caatagttca aatgtttcaa ttgacgtttc tactggtgca ggtgttggag gtagcactgc
     3901 aggaactgca accattacag cgactctaat ttctccatct accttccctg gggttgcaaa
     3961 tggtggttct gcaaccacaa caactcaact taaaaatgta actatcgttt ctattacagt
     4021 aacaggttta tcagaagttg gagctggaac aactgcaaat taccaagcaa gtgcgatcta
     4081 ttcagatgga acttcttctg atatcacatc agttgcaact tggtcagttg atagtgctac
     4141 aaatgcagct gtcagcgata gttcaggaag caaaggtgtt ttaactacta tctctaacgg
     4201 atcggttaac gttcaagcta gctatgttgg tgtaacagga acaaaattag tgacaatctc
     4261 ttctattact atatcttcga tttctgtaag cccttcatcc aaaaccataa ttaagggaac
     4321 gaccactcaa tttacagcaa ctgcaaatta ttcaaatgga acatcggctg atatttccaa
     4381 cacagcagta tggacatctt ccaacacatc aatagctggt gtaaacacat caactagcaa
     4441 tggaggttta gcgcaaggta tcaatactgg ttctgttacg attaccgtag cttcaggtgg
     4501 caaaacagct acagcttctc tatctgttag cgcagcaact cttgtttcca tttctattaa
     4561 tactaatgcg tctatcgcaa agggaactac tagacagtat acagcaacag gaacttattc
     4621 tgatggaact gtattagata ttagctcttt agtaacttgg tcatctagtt caggttctgt
     4681 tgttgctatc agcaatactt ctggaacaaa aggtcttgct acaggatcat ctcaaggcag
     4741 tagcacaatc caagcatctt taaatggggt taatagcaat acttctaccc taactgtaac
     4801 ggcagagaca cttacttcta tcagtatagg atctaacgca tctgtcgcag ctggtggaac
     4861 aaaacagtat agcgcaacag gaactttttc ggatgcaacc actcaagata ttacttcttt
     4921 agtaacttgg acatcttctt ctgccgcaaa tgcaacaatt agcaacgctt cctccgataa
     4981 agggaaagca tatggattag cagcaggaac aaccaatatt accgcttctc ttcctaatgg
     5041 gacgaatggt attactacga gcggtggaac tattgttagt aacacatccg ttctgactgt
     5101 tactgcagca agcaatggat caacggcatc tgtttctgct gtgactgtgg gagcttatga
     5161 cgtaacaatt ccttcaggaa tcaacacaac tacaacacct acaggaaact tctcaggact
     5221 cacatataat actgcgaata acgctgctgg gttcattgct caaattccgg actatagtag
     5281 cccaacattt acttgtcaag cgcaaggaac agcaattcta ggaaagattt tagcggatag
     5341 ccctgaccta gtttcctcct attcgcaaaa ctctgtaaac acagtaaact tcactggtgg
     5401 ttgcacaatt acatacaacc tatcaatcac aactagttct tccaaaaagg ctactgaact
     5461 ctccaaccat ttaatttcca atgttggtat tttagcagtt gggggtggaa gtgttacaag
     5521 cttgccagca agccaatctg gtgaaactgc taccactgca tttagagcaa ttgttcaggt
     5581 tacttataaa acaggaacag gtggacaaac aatcggaatc ggggtaacga attctgctga
     5641 ttatgcagca aaccaagctc tactcgaaag cttcttaggt ggaacaaaca ttgcagtcac
     5701 aggaactacc tatctaacaa aaacagaaac gaagactgga aaggctatgc ctaaagtaga
     5761 cttcgtatgg gtgattgata actcagcatc tacttcaacc attcaaactt ctatttctgg
     5821 agcagctaca accttcttta ataagttagg tggtaaaaac ctagactatc gtttaggtgt
     5881 aatagcgacc gggggtgacg gaacagatgc aactcgcaca gacaaatgta caaatccagt
     5941 aacaccgggt gaacaactta gatctaatgg atggatgact tcttctaata cagccgcaga
     6001 attctctggt tccggtggga caggtgtagg atctaaaggt tgtggaactg agagtggtat
     6061 ctattatgct gctaaatctc ttgggtatcc aaatagtatt acctatacaa caactcctac
     6121 tgtaactcca agatctggtt cttcttgggg gtctgattac aaacttgtat tcgtaatcgt
     6181 gagtgatgaa ggagaccaat ttagatgtta caatggaact tactcggcag gtagtaactc
     6241 aagcggaact ccatgttctg gagcaactgg taatacagac ttcgacaata acaataacgt
     6301 atttgtgaac aatggttaca aagttcatac aattactgga ttagatagtt ccggaaaccc
     6361 aggatcttgt tcagtatcag gtggaccgtc tacatctcaa ggaaacgtta cctggcctgg
     6421 ctataaaaac ctttctactg caacaggtgg tgtagcagcg agtgtttgta gcactgatta
     6481 caatgcgatt atggatacta ttgtgaatgc tgtagcaggg gacagtggct atatattagc
     6541 taaaaaccca gtatcaagtt caattacagt taaggtaaac ggatcagctg tagcacaaga
     6601 ttcaacgaat ggttggcaat atgttcctgc aacaaattca ataatcttct ctggtactgc
     6661 atggcctgca acaaacgcat caatagaaat aagctacttg tattcttctg accaaggagc
     6721 attcttaaag atggattcta atagtagctt aatggcttac atctctcaaa cagcaaaaag
     6781 cagtgctgca agaggaactg ctgcgactat agcgcttatc gttggtgcta tcttagcagg
     6841 aagactctgg agacgtagaa atagcaacaa ataaatcaat ctagatttct tttcagaaat
     6901 gaagcgtggg ataaaaccca cgcttttttt atttaaacga aacaaatctg tttattttct
     6961 attcaaattt ccataatgat aaactttatt atgagagtaa aaaagagaaa aatctaattg
     7021 taaagatcct tcttgatttg aataataaac tcaccttata tttgcaggat ttgtaaacga
     7081 gttattgatt cataaaaaca aggattatgt ccgggtgaag gaaacattca aacgttatta
     7141 actatgatcc atgtaaaggt ctgttaggat tagatctttg attcgggtaa agaaaactaa
     7201 ttatacaaat cggtaaatac ctagacattc agattaatac aaaacatact tcacaatagt
     7261 ttgaaacgat aaatcctata tttgataaat gaggtatcgg atcaaatagg taacaattag
     7321 attttaagat tagtttttaa atctatcttt tatttcgttc ttcttagatt cacttatttt
     7381 tagcacttca gtaagagtat ctagaaattt ctcttcattt ggatccactt ctctgtcacc
     7441 agtataaaca gcaaaacaag caagttctaa gcatagttct tgtttttttt ctttctcgaa
     7501 ataagcagca atcttattca aatcgagggg tgcagaagcc gcttgccttg catgctccat
     7561 aaattcttct ctagtatacc taccctgaat cggtttaaaa atatctatat catagagctc
     7621 tttaactcga tctgcaaacc agactttctc ttcataattt aaaaccccgt cagatctcaa
     7681 ggctcttaaa taaagactaa tataaactga gattaaataa tcatcagata gttctttttg
     7741 aactttccgg atataatcgt taaatcctgt tgaattccca actgcacctt tagatggatt
     7801 gttttgattt gactgtgatt gactcattgt tttttcctga acggattttc cctaaatatc
     7861 ggtagaatgc aagtatttaa ttattttgct aataggtctt cctgtatttt cgatcgaacc
     7921 aattctgtca attcctcggt tgacatattc tcgaaaacag agggagaaat ttcaggcaat
     7981 acagtaatac tcatatcatg tttaccctgt agttctatac caccctttgg aagagcgtga
     8041 gcagttcctt ttaaaacaat aggcaatata ggcgctttgg tcgcttttgc aatttcaaaa
     8101 gcccctagtt taaaagcctt taatgttccg tctaacgacc gtgttccctc aggaaaaatc
     8161 attacagagt tttgattaga gattgccctt atacaatctt ttatcatttt tgcattactt
     8221 tttacttttc cacgatcgag ttcgatataa tcattaaatc gcatattcca accaataaat
     8281 ggaaccttaa aattttcaat ttttgatacc catttgaaat gagaaaataa gcgaaacatg
     8341 atgagtatat ccaataaaga ttgatgatta gagacacaaa cataagcctt tttagaatcg
     8401 atattttctt ttccaaaaat tcttatattc cataaaggat taaaccaagt ataaagagat
     8461 gcccaaaagc aagtaaactg atgcaatagc cattttcttt tacgatcaaa tattagagaa
     8521 aaaacccaaa gggtaaaagc tacaaaaaat agtagtattg aagttattgc cacaaatccc
     8581 caaaatagaa aagaaaaaaa tttattcatt caagtatcct tttgtaatca ttcacgatag
     8641 atcattctaa atattctcca aagaaaaacc gtttcaaact tcttgaatat ttctgatctc
     8701 ttctaatctc cagagactta ttaaagccaa ctttcaatat tttgttatca gtttttttta
     8761 ataaccaacc attccttcaa atttagaatt acctatttta aaagaaagaa atgagccaat
     8821 tagagtaatt tacctaaacg gaagaactct ttaaagaaaa caaatcttta ataaccagaa
     8881 gtagttggat atccagcata accgcctgtt gaagttccga cagctggagt cgctggtgga
     8941 gttccgacgg ctcctgcaga cgaagttccg acagaagagg agcctccatt gtttccactt
     9001 atagaatttt gttggcgata tatatttgaa ttagaatttg tttcgttatt atcaatcaaa
     9061 tcccgtatcg gttgattact gctaggactt tctacttgaa tgctattatc attacaagca
     9121 aatagtaata ttatcaatag gtttaacaga atcttcttca tttcgatcct ctaatattaa
     9181 ggtaaatctt tattctctgg atacttttta attaaataat ctcgattttt ttcacgaagt
     9241 tttgctgtat ctaaattggc atcttcctct gaagcataat ctccaattaa aacacgatag
     9301 gcggttccat tcggttcttt atctgcttta ataaaaattt tgctaaatcc catatcagtc
     9361 atatacttac aaaatcttaa agaattaaaa atatgagaaa aggaaccgac ttgaattcca
     9421 tatccttttg gattttgaat cattccatga atgttgtaaa tgtatccagc cttgaaaatt
     9481 tcagatgagg cttctttcaa atctacttta ggtttctcag gttcaggttt tacattttca
     9541 tttgcattcg ctgcaataga tttagcgggt aacggttcag gatgtgttgt tttagaagga
     9601 gtctcttctt ctttggattc atgttttgct tctgtctttg caggtaaaac gacagtatcc
     9661 gcagtagatg ttttatttgg ctttgctggt gaagtaggag ttgcaataga cttagtatcc
     9721 tttacgggtt catttggaag ctctaccact tcgagagtaa catttgcaac accctgtgaa
     9781 atcatatcta attcttttgc cgcagccttt gaaacatcta gaattcgaac atgatgaaaa
     9841 ggtccacgat cattgattct aacaatcacg cttttattat tttttaaatt tgttacttta
     9901 attttagtat tcagtggtaa ttctcggtga gcggcagtaa acgcattcat atcatatttt
     9961 tcaccgctgt ttgtttttgc gccatgaaaa cgatcgttat aaaaggatgc cttaccagac
    10021 tgagtaaaac caacttgtgc atataattga taagatataa caaagcataa aataaataaa
    10081 aagcgcataa ttatttcctt cattagagta tttttagttt aactagttct ttaatcaatg
    10141 cttttgttgc atcaccaatg acaggcaaat tccaaatata tggtaatgta aaatcaataa
    10201 caacactatc atcctccgtt ctgcctttcc ctccatccac aaagtcttgc caacgaatta
    10261 gatctacttt aaatcgaata tgaattccct catttccttg cacagtaaga gcgtttactt
    10321 ttttcaattc aatggctttc tcttgaatat ttttatcaat cttatcaatg acctcaaaag
    10381 ctttttctac ttttgctttt ggtgatttat gcgtattagc cgcttttttg ggcttagcaa
    10441 ctttttcttc tacttgattt gcttctaaat ttacgttgtc ttgttcttct ctaacgatca
    10501 ctggatcaat agagttatct aatttttttc ttttcttagg caccgtcatg tgttttctcc
    10561 gctaactttt gtaaaataga atgtgctatt ccttcgacat cctcaatggc acgagcacca
    10621 cctacataaa gtcgtttatt atttttaatt gtttcaacat attcaagaag agtcataccc
    10681 tctcgattaa agttttcata tattgatcga aatggataaa ctacatccaa acatccaata
    10741 taatcaggaa gtatatcttt aaagtattct attattttct ctctgttgga ttgagcgtta
    10801 gtaaacgtag gtaaaataaa aaaatttccc tttttaattg gataggatct gataatttct
    10861 tttataatag gaagtaaatt atctgcaaaa gcagactcat cgttagttga tgtcctcatt
    10921 ggaattatta ctatatcgga aattgcacaa gcaaactttg tatgcaattt actagtgctc
    10981 tcgcccggta tatctaaaac aagtaattca ttatgagtgg caatattttt aactccatct
    11041 tgtattactt ctttctgagt gcttgagata tgatgaaaag aaactttttc tgaaggtcga
    11101 ttttcttcag tcctttcctg ccaccaattc tttaatgttc cttgaggatc caattccact
    11161 aaagcaatgg attgaaattt actttgaaag gcgtttgaga aagtagtatt caccgcaaga
    11221 gtgctcttac ctgttccacc ttttgtttgc acaaaactga ttatcatatt acatccaaat
    11281 tttatttaaa atctcatgtt gttattttaa gatattactt taagctcatt gtcaaatcag
    11341 atcaatagaa ctcgctgatt gcgtaagtca taaagcagtt tattggaaag caaagtgctg
    11401 acttactcat cagaaaacta ttcattttta accaaatttc catgagtaaa aaagaatatt
    11461 gattcggtat gaatacagaa tttcaccatg aaaccattgg tcaacgattt aaaatagcct
    11521 aggctttatt taaggatatc tagatttaaa ctagaatcca cttactagga ctattattat
    11581 caatctgaat agattttaac agaaaggctc tagcaaagtg aaaattaaga actcacgttt
    11641 cttcacaccc tgaactcttt tatccttaag taaacccata ccacaagcta ttgattctat
    11701 tagtccatta gttttttcta atatattcct tctcaaaaga attcacaaac ttaaatatca
    11761 cttttaaaaa ctctttccgt atctagacgg ttgcacaaaa agaatgtttg tgattataag
    11821 gatgaatttt tctttgatta aaatttctga ttgcagaaag aacattaaaa gaaaaaaaaa
    11881 ggataatact ttttaaccga ggcttgctat gaataataaa atctctccta agaaaaatac
    11941 attattcgaa ttcatgaaag atgccagaac aaaaaatgca aacatagttg cacaaatgtt
    12001 tagaactggt agtcctagcc tacaatcaat tacatttcaa gaagtctatg aaaagattca
    12061 attaattgca tttggactca tcaagttggg aacaaagaaa ggagataaag taggacttgt
    12121 tgctgatagc ggtcatcgtt ttatgtggct tgcaatggga attacaaaca ttggagcggt
    12181 tgacgtccct agaggaaccg atgccacttc ggaagattta ctctacatat tcaaccatgc
    12241 ttcttgtaaa attatcattc tagaaaatgt gaaagcttat gaaaaaatcc aaaagagctt
    12301 aagcaaactt tctgatttaa aatatattat attttattct gaccccggaa aaatcaagac
    12361 tccaagtaaa gtagaagttc taacacttga tgctttaatg gagctaggag aaaaatatag
    12421 aaaaactaaa ccagatgcgt ttgaaaaaat gggagaagca attgatccaa aagatttagc
    12481 aaccataatt tatacttctg gaacaacagg aactccaaaa ggagtaatgc tctctcacaa
    12541 taattatgtt tggatgtcga caaaattaga agaggcgtta cgcgctacag gtttagaact
    12601 gcaaggagac gaaaccacac ttggttatct tcctccttgg catataggag acagattatt
    12661 tgaaagcact tgcctaggaa ttggagttac aatggcattt accaatatag caaatctagc
    12721 ttccgatcta aaaactgtaa atccaacctt gatgttctct gttcctcgtg tttgggaaag
    12781 cttctacaat aagattttag aaaacgtaaa aaatgctcca ccccttcgaa aagcattatt
    12841 caacttagca tctagtgctg caatgaatta tacccataac agagattatt taagaggctc
    12901 agctctcatt atagaacctg aaagttttac taaaactctt atcaataaaa ctaagtctct
    12961 cttcctaacg cttttattat ttatcccgta tcaattatct aaagtaattc tttcgaaagt
    13021 taaggctgca cttggaaata gaatccgctt tgccttttca ggtgctggcg ctcttcctta
    13081 tcacattgat cgattctttt attcaatcgg tcttccgatt ttggaaacat acggaatgag
    13141 tgaaacaaca ggagtgtctt gtatgcgaga tgtcgcaaga cctgtaatcg gaactcttgg
    13201 aaataacctt agtgaaatag gactaaagct aatcgatgaa aaaggaaatg aaatcacaaa
    13261 accaaatgta aaaggggtag cctaccataa aggacaacat atcatgatgg gctactacaa
    13321 agatccagaa aaaacaaaag ccgttctttc gcctgacggt tggatgaact ctggagacat
    13381 tttaattaga actgcaagcg gacaactcaa atttgcaggg cgggcaaaag acacaattgt
    13441 tctacttggc ggcgagaatc tagaaccaga accaattgaa tttgcacttg ttcaaagtga
    13501 attcattcat caggcaatgg ttgtgggaca ggacaaaaaa acgttaggag ctttaattgt
    13561 tccagcattc gataaagtgg aaaaacattt taaatcacaa aatattgcac tgcctgcaaa
    13621 tagagaagac tggaaaacag atcaaaaaat tttaaatctt tttaaaaatg aaattaaaac
    13681 attggtttct aatgaaaaag gattcaaagc atttgagaaa gtaactggat ttgcaattat
    13741 taccaaggaa tttgaagctg gaaaagaact aacacaaaca ttaaagcttc gcagaaacgt
    13801 tatgtttgaa atgtatgcaa aggaaataga agggatttat aaatgaataa ttctttccag
    13861 tctaattttt tggattggaa tttttattct ttttataggg acaatgcctg catccatttc
    13921 cacagcaata gcctcgtttc aaatgatact tttctgtgaa aacaacaaaa ccattttcta
    13981 tataatcagt ctgttgcgaa atgttaaaga attaaaagtc agcctttaaa aat
//
LOCUS       DAOQOS010000134        13899 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_2267_length_13899_cov_6.923866, whole genome shotgun sequence.
ACCESSION   DAOQOS010000134 DAOQOS010000000
VERSION     DAOQOS010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 13899)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13899)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13899
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2267_length_13899_cov_6.923866"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3602
                     /locus_tag="PLF66_21445"
     CDS             <1..3602
                     /locus_tag="PLF66_21445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66668.1"
                     /translation="GNPGASRTVNLVSGNTLAITLSPASLVFNSSNWNVPQTVVLTGV
                     ADANTVSEAVTITGSGVSVGTATVNVNTVDTNTMNIALTPASTTVGEGSSTTFTVNLT
                     QEPSPSLVVSFASSNTTSVTVAPVSITFDNVCPGAQCWSTLRTITLNGVVDANQTSES
                     VTITASAPAVPNASFTIATTENDAQPVFSGVTTVTEGGTALLSVSLSGNPGAARTLNL
                     TSSNPAAITLSPATLNFTAANWNIPQAVLLTGISDSNASTESVTITGSGVDLGTASVT
                     ISTVDANTMSIVLSAPSTIVDEGSSASFNVKLSQEPSPSLVVSFASSNVASATVAPAS
                     VTFDNVCPGANCWSNFKTITVTGVEDINETTETVTITASAPAVTNATINFDTTDNDSK
                     AVFSGGATITEGSTSVLTVALSGNPGTARTMNLTSSNLTAVTIAPTSLNFNAANWNVP
                     QTVILTALADTNTASETVTITGNGTDLGTSTTSISTVDTSLMNIILSAASTTVSEGST
                     ATMNVKLSSEPSPSLTVTLSSSNAGAISVSPLTLTFDNVCPGAQCWSANQSVTLTGVE
                     DANQTSETVTISASAPSATSASLDFKTIENDTGIVLGGATSVTEGGIAFVTVVLTGDP
                     GIDRIISLASSDTSALLVFPPSLTFTSSNWNTPQIIQVTGVSDVNTIGETVTITASGT
                     GVTSGTGTVTTVDTNVFDLVVTNQSGTTTVNEGSSLTFAVNLNYSPPTSPYVVTISAP
                     QTSPAVPGYVATPTYVIDPANSGIGSTTLTFTTANYATPQTVTINAPENYYIDNKTTT
                     LSFSGAGVTTKNYSITVVDNDPVEQMLVDDGMGPSCNDVSTSYDGVKMLVAAQCSNGG
                     TFNRVKAFRCESNFSYCLDTNTPTLDNGGDNNARSLTALYFTGQAMIVSENKSNGRMN
                     FFTGPLGSWTYYDMSDVAKFNLGNNTAISPRAAWDSTNNKVIVAGSYGSGIYLYSFDK
                     FGNSLNTPVNIAASNVDPGIMIETITDTVNSKVTVTFQYQPTPGQYALSFVQCNLDLT
                     GCSAPATISSLVGLMADGLPLWIKHSQMIYDSTTNKIYIITSMGNTEGGVDTIPKLVI
                     CNPDMTGCTAQTINTGNRSGLKTKIAVDNYNNKLLILAYDATQSYSVVLNRCSLTGTG
                     CTRRNLTNIFGMNIGSSIANPFIDTANKKLRFVAINNASGGVLTMYSMLLFID"
     gene            3618..5066
                     /locus_tag="PLF66_21450"
     CDS             3618..5066
                     /locus_tag="PLF66_21450"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HMZ66669.1"
                     /translation="MKRLILIIFLLIFSIENCSEISFRSDPLSLTLVKYENRSSNKYI
                     FLAHEGDDCHLLQHKRIHNFFFFFPVNSFSREELIEISQQKMIRYKNVMRLGDFVWTL
                     FLIPLTFVTYSIEVETCNTGMVAVTRKDPLYQESLRTKTPTEAEENLAKLEKNRRRQN
                     SPIREKSINPNANEQNLDLVKERNNSSEIQESNESKIKENRVINNPSNFSSDLERKKD
                     SEKTKIEPENESRDYKNKFYSEDRKIYPYEQNKSSSYYYSQNQSNHPNEQSSIQRDKN
                     KSHYKESNLLDKSTQTDQITANDSNQSNGKKKKKKGKANGEANPDLPAEEVWDKASVI
                     DETPEPKEPFNPSIYKKDGSLPIIWNYKNSITNRINENIKPFSILFGRNETEITNEES
                     QKLEAFANEYLESYGTSKILLIGHSEWADGKGVKLTLSQLRAEAVRDFLIEKKVPKEN
                     ILLTFSGGLWSEKNEAKTGKSFSRRVDIVFLY"
     gene            complement(5067..5633)
                     /locus_tag="PLF66_21455"
     CDS             complement(5067..5633)
                     /locus_tag="PLF66_21455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66670.1"
                     /translation="MNRVKKIIFVLFLFTSSLFSQEVGFNDSVKLKNGKILENVKTVT
                     IKDAIVAVDSSGVANVYSKKEVLEVVKNGKLSEVKQVSKGISSKTSSGVWTEYSGRRP
                     WVNAVEYCESIGARLPTGEELKTAYESGLTKSWEKDGCMYWTVPPDYSKEKRSRAYTI
                     GGICSISKEYQIYSQGYWYLIGDVRCIR"
     gene            complement(5732..5986)
                     /locus_tag="PLF66_21460"
                     /pseudo
     CDS             complement(5732..5986)
                     /locus_tag="PLF66_21460"
                     /inference="COORDINATES: protein motif:HMM:NF016876.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(6026..6202)
                     /locus_tag="PLF66_21465"
     CDS             complement(6026..6202)
                     /locus_tag="PLF66_21465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66671.1"
                     /translation="MFNFVNYLKFKSKNVEEEKAELRKRIEFAKKNPDKLLNKKEAIK
                     KLEKSLGRKIQIKS"
     gene            7082..11455
                     /gene="cas9"
                     /locus_tag="PLF66_21470"
     CDS             7082..11455
                     /gene="cas9"
                     /locus_tag="PLF66_21470"
                     /inference="COORDINATES: protein motif:HMM:TIGR03031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II-B CRISPR-associated RNA-guided
                     endonuclease Cas9/Csx12"
                     /protein_id="HMZ66672.1"
                     /translation="MNKYISPIAVDLGAKNTGVVFSHYKEGEEPISHRGVTFVLDANN
                     FTFSPKDRLVNRHRMRGEQRRKLAKRLFFLILDKEYGLKQEQSKIWEFIRGLLNRRGF
                     TYLQEESESDEKITVPLDFAKYFFPKEFENCKKDVLDFIREQADEEEAKALTVWKTLR
                     DTDKKEELQKFLAEYSDYTRRDSESYLKQIKETAKGLIKEIEQGNKPRKKYLEEIWID
                     IQKKSELTKLLAKSKKPISVKEFYHLIGHISNIQLRGLRKYFNDINMSKGNDYWDEKR
                     LTKYYVLWLKSWHAPKDKPSRQYQSDLIRQVEKEGKSILQIFFDNDPKISIPPFEDQN
                     NRRIPTCQSLLLSAIELDKRYNGWRDIVKELTEKQDFYKVDNIQSILDEQKKSLQGFS
                     HNNQSGKNLTKEKIPFDFDSILFQRVLETTKSLDLYKLRLLVQVKQLKQDDLASSLEG
                     REKLKKIIQNAEENLRAVLKPEQKKLLLTIAEKFYQEITEARAGIWDENNSILAKCNS
                     KTKHRNKMQKEVVEQVLRFKFNSEISFEYFKENIWYHPIKGKSTVKSLCENIEKERKE
                     IGNHFNEAYQQYTRKVFAIAKSESKDLALINKSLSKEKGNNKSFSTIYENIELIANFI
                     GSSLKQSPLDIKSYKNPFSLAQLANILEGDQAGFYKICSACQKENSWRAELEDLENSK
                     KEVSRVAKATQLSADTGRPFDGQIDRIVTRIAKEVTNKKIHQLKGHFKDGDKIELPIL
                     MENNQFSFTEEALDLKKASKKAKEKSKRNIEKSESRLLSKENRIKSDSGGICPYTGSA
                     LGEDGEIDHIIPRSVTKGTILNSELNLIYCSHKGNQEKGNKEYTLQNINQKFLKHHFK
                     TTNLDSVIAEIDKIILEVAQKNREAQRNYIIADSLEPYQRICLKLALLDRSLRNKVIP
                     YLQTHSKALVNGTQAWFYKKLKEELESRLQSEYKKSEIKIVPHYFGGRDDDFILGELR
                     NKLASYNKNYKKAEIQTEGSHIIDASMLFAHSLLQSDKNKGFLKSPLLKEELQNEGEW
                     IESLLPEEIEVKYIERTAKYRKQNPNSNSLFKAGMYAERFISLLVDNKGIRFGFTPDK
                     ASPYIDTKFNEEIFEKLKPFLLYRGEFIQENLEHFLKLANHPKYSLFKLHRNKVKEYL
                     QTCSKDDELGNFLESLRYITQNKKIFDEKLNLNIDEKTLSKKEKFEIKFGKKKITSFE
                     IIMPYVDEWNKLKDYLKGCKPENIENKAREFFTKDLIRNKSKHGTVSRKLSLPVKSEP
                     SGGFRIKRTDDFNELIYQVQAGETTNIGFEKNNNEVNFSNSILAKNYLSGNVSAYKLE
                     DKISANDFVYFDEWRNLPIANDLKDAGILKLEMAPGTKSRCYIKATILLEVLLNILFD
                     GKEKSSLKSKIYKLNLYDKKDKEGFVKLSRFLETHISKQRDFISINKLNNKLVEIEYV
                     TDGFPSSLKSLYNQGTKIENP"
     gene            11452..12447
                     /gene="cas1"
                     /locus_tag="PLF66_21475"
     CDS             11452..12447
                     /gene="cas1"
                     /locus_tag="PLF66_21475"
                     /inference="COORDINATES: protein motif:HMM:TIGR00287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas1"
                     /protein_id="HMZ66673.1"
                     /translation="MRHLTITTFGCFIGLESKRLVVKEKDKVIGEYPIARLKTVQVAA
                     SGVSFSSNLLYELSLRGISFFVLDFKSIPVLVLSGTQQHAVVELRKHQFQFSESNKVP
                     ELCQKIILGKLKNQRSVLLYFSKSQNINLKNSESIQKGVLSLENTIQELKSADWLANS
                     NWRERLLGTEGGGAHIYWNTLRESDLFPDSFEARKGRGASDIVNQALNLGYSILMSYI
                     WNAVIKSGLEPYLGFFHVVRPGKPSLILDIMEEYRAWVVDRSVIKLRHLLEKEKTLTP
                     EIKKRVIEEIHETFSKTYPFYGKKLKLETMLQRQVYRLCGFIYGKKKYKAISFKW"
     gene            12454..12744
                     /gene="cas2"
                     /locus_tag="PLF66_21480"
     CDS             12454..12744
                     /gene="cas2"
                     /locus_tag="PLF66_21480"
                     /inference="COORDINATES: protein motif:HMM:TIGR01573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HMZ66674.1"
                     /translation="MYIEILVCYDITSNKARSKLVKELKDMGLLNVQESVFWGRILPA
                     ELKSIQRFFDELLKKGEDKAFILKANFSKQIKSNSFGYDNPEIYEERDYEIF"
     gene            12747..13331
                     /gene="cas4"
                     /locus_tag="PLF66_21485"
     CDS             12747..13331
                     /gene="cas4"
                     /locus_tag="PLF66_21485"
                     /inference="COORDINATES: protein motif:HMM:TIGR00372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas4"
                     /protein_id="HMZ66675.1"
                     /translation="MYEDSLTIVQLRQYFFCPRINWFQVNSYNLPKENIWMSQGNEYH
                     VHRENLLKKKLLKRFQNKNCKLEYRIPVYSSKYKIHGIVDAIVRADNDAYPVEFKLNP
                     ASKSKGIFFQMLGYAVCLEEKYNLKIEKGFLVAGKNPKVVEIKFSDEDKKELAQIVEK
                     IKEIRESDFLPESSASKEKCIQCEYLNFCNDRNL"
     repeat_region   13522..13845
                     /inference="COORDINATES: alignment:crt:1.2"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=13522..13558
                     /rpt_unit_seq="ctaacatccaattacaacaacgacctgcaactgaaat"
BASE COUNT         5000 a         2426 c         2385 g         4088 t
ORIGIN      
        1 ctggaaatcc gggtgctagt cgaactgtaa atctagtttc tggtaacact ttagccatca
       61 ctctttcgcc cgcttctctt gttttcaact cctcgaactg gaatgtgcca caaaccgttg
      121 ttttaacggg agtagccgat gcgaatacag tttcggaagc agtcacaatc acaggaagcg
      181 gagtctccgt cggaactgca acggttaacg tgaatacagt ggatacaaac acaatgaata
      241 tcgcattgac tccagcatct acaactgtag gggaaggatc ttccacaaca ttcactgtga
      301 atctaacaca agaaccttct ccttctcttg tggtaagttt cgcttcgagc aatacaactt
      361 cagtaaccgt tgcacctgtc tcaatcacat tcgataacgt ttgtccaggc gcacaatgct
      421 ggagcacact gagaaccatt acgctcaacg gagttgtcga tgcaaatcaa acgagtgaaa
      481 gcgtcacaat cacagcctct gcacccgctg tccccaatgc atcctttacg attgctacaa
      541 cagaaaatga tgcacaacct gttttcagtg gagtaacaac tgttaccgaa gggggaactg
      601 ctttactttc tgtaagcctt tctggaaatc ctggagcagc gagaactcta aacctaactt
      661 ctagtaatcc tgctgcaatc acactttctc cggcaaccct gaacttcact gcggctaatt
      721 ggaatatacc gcaagcagtt ctattaaccg gtatatcgga ttcaaatgca tccactgaat
      781 ctgtaaccat aaccggaagt ggagtagact tgggaactgc ttccgtgact atttctactg
      841 tggatgcaaa tacgatgagt attgttttat ccgcaccgtc aaccattgtg gatgaagggt
      901 cttccgctag tttcaatgta aaattaagtc aggaaccatc cccttctttg gttgtaagtt
      961 ttgcttcttc caatgttgcc tcagcaactg ttgcacctgc ctctgttaca tttgataatg
     1021 tctgtccagg tgcaaattgt tggagtaatt tcaaaacgat aaccgtcaca ggggtagagg
     1081 atatcaatga gacgacggag acagtcacga tcactgcatc tgccccagca gttacgaatg
     1141 cgactatcaa ttttgatact acagataatg actcaaaggc agtatttagc ggtggagcga
     1201 ctattacaga aggttctaca agtgtgttaa ctgttgcact ttctggaaac cccgggacag
     1261 ctagaacaat gaacctaacg tctagcaatc tgactgcagt aaccattgca ccaactagtt
     1321 taaattttaa tgctgccaat tggaatgttc cgcaaactgt aattctgacc gctctagcag
     1381 acacaaatac agcttcagaa acagttacaa tcacaggaaa tggaacagat ttaggaactt
     1441 ctacaacttc catttctaca gtggatacga gtctaatgaa cattatcctc tctgcagcat
     1501 ccacaactgt aagtgaaggt tcgacagcca caatgaatgt aaaattaagt agcgaacctt
     1561 ccccatctct tacggttaca ctctcttctt ccaatgcagg tgctatttcc gtaagtcccc
     1621 ttaccctaac ttttgacaat gtctgcccag gtgcacaatg ttggagtgca aatcaatccg
     1681 tgacgttaac cggcgtagaa gatgcaaacc agactagtga aacagtaaca atttctgctt
     1741 ctgctccgag tgctaccagt gcaagtctcg atttcaaaac aattgaaaat gatacgggta
     1801 tcgttttagg aggtgcaaca tccgttacag aaggaggcat tgcttttgta actgttgttt
     1861 taactggaga tccaggaatt gatagaataa tcagtttagc atcgagtgat acatctgctc
     1921 tcttggtatt tcctccttct ctaaccttca catcctcgaa ctggaatact ccgcaaataa
     1981 ttcaagtcac aggggtatcc gatgtaaata caataggcga aacagttacc attacagcta
     2041 gtggaacagg agtgacaagc ggaactggaa cagtgactac agttgataca aatgtatttg
     2101 atctagttgt aaccaatcaa agtggaacaa cgactgtcaa cgaaggaagc tctcttacat
     2161 ttgcggtaaa tctaaattat tcaccgccaa cttctcctta tgttgttaca atcagtgctc
     2221 cccaaacttc ccctgccgtt ccgggttatg ttgcaactcc tacttatgta atcgatccag
     2281 caaactctgg aattggatca acaactctca ctttcacaac tgccaattat gcaactccgc
     2341 agactgttac aattaacgca ccggaaaatt attatattga caataaaaca accactctaa
     2401 gtttttcagg agctggagtt actactaaaa attattccat cacagtagtg gacaatgatc
     2461 cagtagaaca gatgttagtc gatgatggaa tgggtccttc ttgcaatgat gtatccactt
     2521 cttatgatgg agtcaaaatg ctagtagccg ctcagtgttc gaacggcgga actttcaatc
     2581 gagtgaaagc atttagatgt gagtctaatt tctcttattg tctagataca aacactccca
     2641 cactggataa cggtggggac aataatgcaa ggagtctaac ggctttatac ttcacaggac
     2701 aagccatgat tgtatctgaa aataaatcca atggaagaat gaatttcttc acaggacctc
     2761 ttggttcatg gacttattat gatatgtcgg atgtcgctaa gtttaactta ggaaataaca
     2821 ctgccatttc accaagagcc gcttgggatt ctaccaataa taaagtgata gtagccggta
     2881 gctatggatc cggaatctat ctctattcct ttgataaatt tggaaatagt ctaaatactc
     2941 ctgtaaatat tgctgcatca aatgtagatc caggaatcat gatagagact atcacggaca
     3001 ctgtgaattc aaaagttaca gtaaccttcc aataccaacc aacgcctggt caatatgcgc
     3061 tgtcctttgt tcaatgcaat ttagatttaa caggatgtag cgctcctgca actatttcta
     3121 gccttgtagg gcttatggcg gatggtctcc cactttggat caagcattca cagatgatct
     3181 atgattctac cacaaacaaa atctacatta tcacttctat gggtaataca gaaggaggtg
     3241 tggatacaat tccaaagctt gtaatctgta atccagatat gacaggttgc acagctcaaa
     3301 caatcaacac aggaaaccga agtggactca agacaaaaat tgcagttgac aattataaca
     3361 ataaattatt aattcttgcc tacgatgcca cacaaagtta ttcagttgta ttaaatagat
     3421 gcagtctaac tggaaccggt tgcacaagga gaaatttaac aaatattttt ggaatgaata
     3481 taggaagtag tattgcaaat ccgtttattg atacagcaaa taaaaaacta agatttgttg
     3541 caatcaacaa tgcaagcggc ggagttctaa ccatgtattc aatgctatta tttatagatt
     3601 aacgattggt gatgaaaatg aaacgactga tactaattat atttttactc atcttctcaa
     3661 ttgagaattg tagcgaaata tcctttcgct ccgatccact ctctcttaca ctcgtgaagt
     3721 atgaaaatcg ttcttctaat aaatacattt ttctagctca tgaaggagat gattgtcatc
     3781 ttcttcaaca caaaagaata cataacttct ttttcttttt tccagtgaat agtttttcta
     3841 gagaagagct tattgaaatt tctcaacaaa aaatgattcg ttacaaaaac gttatgcgtc
     3901 ttggtgattt cgtttggacc ttatttttaa ttcctctaac atttgtaacc tactctattg
     3961 aagttgaaac ctgtaataca ggaatggtag cagtcactag aaaagatcca ctctatcagg
     4021 aatctttaag aaccaaaacg cctacagaag cagaagaaaa tctagctaaa ttagaaaaaa
     4081 atagaagacg tcaaaactct ccaatcagag aaaaaagcat taatcctaat gcaaatgaac
     4141 agaatttaga tttggtgaaa gaacgaaata attcttccga aattcaggaa tccaatgaga
     4201 gtaaaataaa agagaatcga gtcataaaca atccatccaa tttttcaagt gatctcgaaa
     4261 gaaaaaaaga ctctgagaaa actaaaatcg agccagaaaa cgagtcaaga gattataaaa
     4321 ataagtttta ctccgaagac cgaaaaatat atccttatga gcagaataaa tctagctcat
     4381 actactattc tcaaaatcaa agcaatcatc caaatgaaca atcaagcatc caaagagata
     4441 aaaataaatc gcattataaa gagtccaatt tactagacaa atcaactcag acggatcaaa
     4501 ttactgcaaa cgattcaaat caatcgaatg gaaagaaaaa aaagaaaaaa ggaaaagcga
     4561 atggagaagc taatccagat ttacccgcag aagaagtttg ggacaaagca tctgtaatcg
     4621 atgaaactcc agaaccaaaa gagcccttta accctagcat ctacaaaaaa gacggtagtc
     4681 tacctatcat ctggaattat aaaaactcca ttaccaatcg aatcaatgaa aacattaaac
     4741 cattctcaat tttatttgga cgcaatgaaa ccgaaatcac aaatgaagaa tctcaaaaat
     4801 tagaagcatt cgcaaacgaa tacttagaaa gttatggaac tagtaaaatc ttactcattg
     4861 gccactccga atgggcagat ggcaagggtg taaaactaac gctctctcaa ctacgagcag
     4921 aagcagtccg tgatttcctt atagaaaaaa aagttcccaa agaaaatata ctactcactt
     4981 tctccggcgg actctggtcc gaaaaaaacg aagccaaaac aggaaagagt ttctctagaa
     5041 gagtagatat tgtatttttg tattgattag cgaatacagc gaacgtcacc tattaaatac
     5101 caatagccct ggctgtaaat ttgatattct ttagaaatac tgcaaatacc accaattgta
     5161 tatgctctag atcgcttttc ctttgaataa tcaggcggta ctgtccaata catgcaacca
     5221 tctttttccc atgacttagt taaacccgat tcatacgcgg tcttcaactc ttctccagtc
     5281 ggcagtcttg ctccaatgct ttcacaatat tcaactgcat taacccaagg tcttcttccc
     5341 gagtattcag tccatacgcc agaagatgtc tttgaagaaa tgcctttaga tacttgcttt
     5401 acttcgctga gtttgccatt cttaaccact tctaaaactt ctttcttaga gtagacatta
     5461 gctacgccag aagagtctac tgcaactatc gcatctttaa tcgtaactgt ctttacattt
     5521 tctaatatct ttccattttt taattttaca gaatcattaa accctacttc ctgtgaaaac
     5581 aaagatgagg taaacaagaa taatacaaaa attattttct ttactctatt cattttaata
     5641 tcctaatttt taatcgtttg tttatttcta aaatccaatt aaaataaatt tcgaacgcga
     5701 gcctatgaag ttcaattgtt ggtttaaaag attaattcaa attattcttc tttttttctg
     5761 ctttcaatct tactttccac cgttcttccc tttttttatg ccaaactgcc aacactacta
     5821 caaaagatga tagaataatg tagatgatat tgaaagggaa ttttcttagg ctggcttttc
     5881 ttgcttcttc aaaatatgga gaatatgatt ttggtcgttt gctgatctga tctagcttag
     5941 aaaaaacatc atccataaat tcatttgctg ctttaggact tctctcctca tagtaatgaa
     6001 ctgactctaa atatctgtaa tcgcctcagg acttaatttg tatttttctt ccaagtgatt
     6061 tctccagttt ctttatcgct tcttttttat ttaataattt atctggattt tttttggcaa
     6121 actcgattcg ttttcgaagt tctgcctttt cttcttctac atttttactt ttaaatttta
     6181 aataatttac aaaattaaac acttctttct gaagctcaaa tggtaagcct gtaatatttt
     6241 ttttaacagt ctgaaatcga ttgaagattg catttctatt tccttcttta aattttataa
     6301 tctatcttct aaaacttctg taaacacatt ttctttgtat taagttcgta ttaacgatac
     6361 acaatctggc atccatctag gagaccaact ggcttccccc tagcataacc caagccagca
     6421 tctagctaga ggatagcttc ttggtatgcg aaatggtagc tagatagtat aacgtcttcc
     6481 cgatgttaat aggaaggaat taaaagatta ccacctattg ccgcgaacaa gcggcaatgg
     6541 attttgggtg gaaagacgat gggcaccgat aaaagatacg atatttcccg aatgcattat
     6601 ttaattcatt ctgctatctt tgtgaatctg taaaatgtgc aactgcgtaa ctttttgaaa
     6661 aatatttttt aaaagaaaaa ttttagtgtt attatcccaa tctcaactcc gaaaatttca
     6721 ttacatacaa tttctaaaaa aactcaggaa tacaaggatg catacggttt tttagcctaa
     6781 agcttctatt ttgtcttgca cttagtttag aatgatagat atttggactt tgtgtttttt
     6841 ctaggaatca gttgttttta ctcataaata ccgcatcaac tgattttagt agtcatccac
     6901 agagcaatta cacaataagg attcactatg aaacactata acgattttac aaacctttac
     6961 tcactttcca agacacttcg ctttgaattg agaccggtcg ggaaatactt ggaaaaaact
     7021 aacacaagaa gatgcaggaa ttttacttcg aaaaacattt ttataaatta gggaattaat
     7081 tatgaataaa tatatttcac caatcgcagt tgatttagga gctaaaaata caggagtagt
     7141 tttttctcat tataaagaag gagaagagcc tatttctcat agaggagtca cctttgttct
     7201 agatgctaat aatttcacat tttccccaaa agatcgtctt gtgaatcggc atcgtatgag
     7261 aggagaacaa aggcgaaaac tagctaagcg gctattcttt cttatcctcg ataaagaata
     7321 tggattaaaa caagaacaaa gcaaaatctg ggaatttata agaggtttat taaatcggag
     7381 aggttttact tatttgcaag aagaaagtga aagcgatgaa aagatcacag ttcctctcga
     7441 ttttgcaaag tatttctttc caaaagaatt tgaaaactgc aaaaaagatg tgctagattt
     7501 tattcgggag caagcagatg aagaagaagc aaaggctcta actgtatgga aaacgttaag
     7561 agatacagat aagaaagaag aacttcaaaa gttccttgcg gaatattcag attatactcg
     7621 tcgagactct gaaagttatc taaagcaaat aaaagaaacc gcaaaaggtt taatcaaaga
     7681 aattgaacaa ggcaataaac ctagaaagaa atatttagaa gaaatctgga ttgatattca
     7741 aaaaaaatca gaactaacta agttattagc aaaatccaaa aaacctattt ccgtaaaaga
     7801 gttttatcat ctcattggac atataagcaa tatccaactt cgcggactta ggaaatactt
     7861 caatgatatc aatatgtcaa aaggaaatga ctactgggac gaaaagcggc taacgaaata
     7921 ctatgtactt tggttaaaaa gttggcacgc acccaaagat aaaccatcta gacaatacca
     7981 atctgatttg attcggcaag tcgaaaaaga aggtaaatct atattgcaaa tattctttga
     8041 caatgatccc aaaatttcta ttccaccttt tgaagatcaa aataatagac gaattccaac
     8101 ttgtcaaagt ttgctcttat ccgctatcga attagataaa cgttataacg gatggagaga
     8161 cattgttaaa gaactaacag aaaaacaaga tttttacaaa gttgacaata ttcaaagtat
     8221 tctcgatgag caaaaaaaat cattgcaagg attttctcat aacaatcagt ctggcaaaaa
     8281 tcttactaaa gagaaaatac cctttgattt tgattccatt ttatttcaaa gagttttgga
     8341 gacaacaaag tcacttgatt tgtataaact aaggttactt gttcaagtga aacagttaaa
     8401 gcaagatgat ctagcttcta gtttggaagg aagagagaaa ctaaaaaaaa taattcagaa
     8461 tgcagaagaa aatctaagag cagtattaaa accagaacaa aaaaaacttt tactcaccat
     8521 tgccgaaaaa ttttaccaag aaatcacaga agctcgtgct ggtatttggg atgaaaacaa
     8581 ttcaattcta gctaagtgta attccaaaac taaacataga aataaaatgc aaaaggaagt
     8641 tgtagaacaa gttttacgct tcaagtttaa ttcggaaatt tcgtttgaat attttaaaga
     8701 aaatatctgg tatcatccaa tcaaaggaaa gtctaccgtt aaaagtcttt gtgaaaatat
     8761 tgaaaaagaa agaaaagaaa ttggaaatca ttttaatgaa gcctatcaac aatatacgag
     8821 aaaagtcttt gcaatagcca agtcagaatc gaaagacctt gcgttgataa ataaatcttt
     8881 atcaaaggaa aaaggtaaca ataaatcctt tagcacgatt tatgagaata ttgaattaat
     8941 tgctaatttt attggatcgt cattgaaaca atctccatta gatattaaaa gttataagaa
     9001 tccattttcc cttgcacagc tggcgaatat cctagaagga gatcaagctg gcttttataa
     9061 aatatgctct gcctgccaga aagaaaattc ttggagagct gaactggaag atttagaaaa
     9121 tagcaaaaag gaagttagtc gtgttgcaaa ggcaactcaa ttatcagccg atacaggaag
     9181 accctttgat ggacaaatag accgtattgt aactagaatt gctaaggaag tcacaaataa
     9241 aaaaatccat caattaaaag gacactttaa agatggggat aagattgagt taccaattct
     9301 tatggagaac aatcaatttt cctttaccga ggaagcactg gatttgaaaa aggcaagcaa
     9361 aaaagcaaaa gaaaaatcga aacgaaatat agaaaaatca gaaagtagac ttttatcaaa
     9421 ggaaaacaga atcaaatctg attccggtgg tatatgccca tataccggaa gtgctctagg
     9481 agaagatgga gagatagatc atattatccc tagatcagtg acgaaaggaa ctattttgaa
     9541 ttcagaatta aatctaatct actgtagcca caagggaaac caagaaaagg gtaacaaaga
     9601 gtatacgcta caaaatataa accaaaaatt cttaaagcat cattttaaaa ctactaatct
     9661 tgattctgta attgcagaaa tcgataaaat aattttggaa gttgcacaaa aaaatcgaga
     9721 agcacagcga aattatatta tagctgatag tcttgagcca taccaacgaa tttgtttaaa
     9781 gctggctcta ttggatagat cactcagaaa taaagtgatt ccatatctac agacacattc
     9841 aaaagctttg gtgaatggaa cacaagcctg gttttataaa aagttaaagg aagaattgga
     9901 gtctagactt caaagcgaat ataaaaaatc agaaataaaa attgtcccgc attactttgg
     9961 gggcagagat gatgatttta ttttaggaga gcttagaaat aaattagctt cttataataa
    10021 aaattacaag aaagcagaaa tccaaacgga aggcagtcac ataattgatg cgtctatgtt
    10081 gtttgctcat tcccttttac aatctgataa aaacaaagga tttttaaaat cacctttgtt
    10141 aaaagaagaa ttacaaaatg aaggagaatg gattgaaagt ttacttccag aagaaattga
    10201 agtgaaatac attgagcgaa ctgcaaagta tagaaaacaa aatccaaata gtaattcttt
    10261 gttcaaagca ggaatgtatg cagagcgatt tataagtcta cttgtggata acaaaggaat
    10321 tcgttttgga tttacacctg ataaagcaag tccttatatc gatacaaaat tcaacgaaga
    10381 aatttttgaa aaattaaaac catttctttt atatcgagga gaatttattc aagaaaattt
    10441 agaacatttt ttaaaactgg cgaatcatcc aaagtattct ctctttaaat tgcatcgcaa
    10501 caaagtaaaa gaatatttac aaacttgttc taaggatgat gagcttggaa attttttgga
    10561 gagtctacgt tacattaccc agaataaaaa aatattcgat gaaaaattaa atttaaatat
    10621 agatgaaaag actctttcta aaaaagaaaa atttgaaatc aaatttggaa aaaagaaaat
    10681 tacctctttt gaaattataa tgccgtatgt agatgaatgg aataaattaa aagattattt
    10741 aaaaggatgt aaaccagaaa atattgagaa taaagcaaga gaatttttta ctaaggatct
    10801 aattcgaaat aaaagtaaac atggaaccgt tagtagaaag ctctccttgc cagttaaatc
    10861 agagccatca ggaggtttta gaattaaaag aacagatgat tttaatgaat tgatatatca
    10921 agtccaagct ggtgaaacaa caaacatagg ttttgaaaag aataacaatg aagtaaattt
    10981 ttcaaactct atccttgcaa agaattatct ttcaggaaat gtatctgctt ataaattaga
    11041 agataagatt tctgctaatg attttgttta ctttgatgaa tggagaaatc tccctatcgc
    11101 aaacgattta aaagatgctg gaattcttaa actagagatg gcacccggaa caaaaagtag
    11161 gtgttatata aaggcaacta ttttactaga agtcttgttg aatattcttt tcgatgggaa
    11221 agaaaagtca tctctaaagt caaaaattta taaactaaac ctatatgata aaaaagataa
    11281 agaaggtttt gtaaagctta gtagattttt agaaacacat atttcaaagc aaagagattt
    11341 tatttcaatt aataaattaa ataacaaatt agttgagatt gaatatgtaa ctgatggatt
    11401 tccatcttct ttaaaatccc tctacaacca aggaacaaag attgaaaatc catgagacat
    11461 ttaacaatta caacgtttgg gtgtttcatt ggattagaaa gtaaaaggtt agtagtcaaa
    11521 gaaaaagata aggtaattgg tgagtatccg attgcccgtt taaagactgt gcaagttgcg
    11581 gcgagtggag tttctttttc ttctaatctt ttgtatgagt tgtcgcttag aggaatttct
    11641 ttttttgttt tggatttcaa aagtattcct gttttagttt tatctgggac tcaacaacat
    11701 gcggtagtag aattacgtaa acatcaattt caattctcgg aatctaataa agttccagag
    11761 ctatgccaaa aaataattct aggcaaatta aaaaatcaaa gatcggtttt actctatttc
    11821 agtaaatctc aaaatataaa tctaaagaat tcggaatcta ttcaaaaagg agttttatct
    11881 ttagaaaata cgattcaaga actaaaatca gcagactggc ttgctaattc taattggaga
    11941 gaacgactct taggaacaga aggaggcggc gcacatattt attggaatac gttacgcgaa
    12001 tcagatctat ttccagattc attcgaagca agaaaaggga gaggtgcttc tgatatagtg
    12061 aatcaagctt tgaatctagg atattcgatt ttaatgtctt atatatggaa tgctgtgatt
    12121 aaatctggat tagagccata tctaggtttt ttccatgttg taagaccagg caaaccatcc
    12181 ctaatcttag acattatgga agaatataga gcatgggttg ttgatagaag cgttattaaa
    12241 cttagacatt tattagaaaa agaaaaaact cttacaccgg aaataaaaaa aagagtgata
    12301 gaagaaatcc atgaaacttt ttctaagacg tatccttttt atggaaagaa attaaaactt
    12361 gaaacaatgc ttcaacgaca agtctatcgt ctctgtggat ttatatacgg aaagaaaaaa
    12421 tacaaggcaa ttagcttcaa atggtagaat taaatgtata ttgaaatttt agtttgctat
    12481 gatattactt caaacaaggc aagaagcaaa ttagtaaaag aattaaaaga tatgggtttg
    12541 ctgaatgtcc aagaatctgt tttttgggga agaattttgc ctgccgagtt gaaatcgatt
    12601 cagcgttttt ttgatgaact tttaaaaaaa ggagaagata aagcttttat cttaaaagca
    12661 aatttttcta aacaaataaa atcaaatagc tttggttatg ataacccaga gatatatgaa
    12721 gaaagagact atgagatttt ttaaatatgt atgaagatag tttaacaatc gttcaactca
    12781 ggcaatattt cttttgtcct agaattaact ggtttcaggt taatagctat aatctgccaa
    12841 aagaaaatat ttggatgtcc caaggaaatg aataccatgt tcatagagaa aatttgctaa
    12901 agaaaaaatt attaaaacga tttcaaaata aaaattgtaa attagaatat cgcattcctg
    12961 tatactcatc aaagtataaa attcatggaa ttgtagatgc gatagtaaga gcggataatg
    13021 acgcttatcc agtagaattt aaattaaatc cagcctccaa atcgaaagga attttttttc
    13081 aaatgttagg atatgcggtt tgtttggaag aaaaatacaa tcttaaaatt gaaaaaggat
    13141 tcttagttgc aggtaaaaat cccaaagtag tggaaataaa attctctgat gaggataaga
    13201 aagagttagc tcaaatagtt gaaaagatta aagaaattcg agagagtgat tttttgcctg
    13261 aaagctctgc atcaaaagaa aaatgcattc aatgtgagta tttaaatttt tgtaatgaca
    13321 gaaatttgta atataagaaa ctctccatgc ttagcgcaga gggagagtca actttgattt
    13381 tgttgttttc agtgctaggt cgttaaaatg actgtaaaat ttcttcgaaa aatagcaagt
    13441 attttttcaa cttttcaatc ttcgaagaaa ctctaaactt agcaccccaa aaagcctgaa
    13501 ttttcaggct caggggggag actaacatcc aattacaaca acgacctgca actgaaattg
    13561 tttctggttt tttaaattct cttactttcg gtttactaac atccaattac aacaacgacc
    13621 tgcaactgaa atacaaagac tatgcaataa tgcaagaggc taacaactaa catccaatta
    13681 caacaacgac ctgcaactga aatttggtgt agctttcttt ttgccctcgt ttacaggcta
    13741 acatccaatt acaacaacga cctgcaactg aaatttagaa ccacgcaaat cagaattacg
    13801 caaatcagct aacatccaat tacaacaacg acctgcaact gaaatagaac agtaggcgca
    13861 taataatcta ctgcctcagg tctaacatcc aattacaac
//
LOCUS       DAOQOS010000135        13195 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_2418_length_13195_cov_6.643455, whole genome shotgun sequence.
ACCESSION   DAOQOS010000135 DAOQOS010000000
VERSION     DAOQOS010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 13195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13195
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2418_length_13195_cov_6.643455"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1078..1995
                     /locus_tag="PLF66_21490"
     CDS             1078..1995
                     /locus_tag="PLF66_21490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002072093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66676.1"
                     /translation="MKIIKGIFILLVLVNLNLLLAQKTETAPAGGAKDAAEPNEAGDT
                     IKGELIADDDTDAGLSDEERKKKKETLKHEAMWKNQDFVGYNKAFEELHKLSKAFANN
                     KYRLALSAYQSGMNTILKMRESVEQYRKEQAEKKRLDEKWYWQKIDRKANEERYIARE
                     KMAAKLAAVTYFTKAINHLDDITNPDLRETDAYKTLLSSVYRSWIMAEYDTQNYPQCI
                     PILELYLEIKENEKEYPAHKYLASCYSFEENTLRKYKAGTDDQIQKLKYKKNVHLLRA
                     TELKYGKGTVEYKHIVDLVNKDEVISVNP"
     gene            2063..2908
                     /gene="prmC"
                     /locus_tag="PLF66_21495"
     CDS             2063..2908
                     /gene="prmC"
                     /locus_tag="PLF66_21495"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004439369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HMZ66677.1"
                     /translation="MEKDTILYVLTKSQEYLGKKQIPNPRLDAELILSDILNLERIKL
                     YSNFDKKLTESEKDIYRERIKARGEFKPVAYITGYKHFYKSIFKVNDQVLIPRPETEE
                     LIEWISKEQNLNQKVSLLDLCTGSGCIGISIKKDFPAVEMTFSDLSEQAILIAEENAK
                     SILGEDKAKFIQSDLFDSIKDSFDFIVSNPPYIPIEEKEILMPDVVNYEPHLALFLED
                     PNHFYNRFLIGAFEHLNPNGVFYFETHQDWASKIGKVGLEIGFKDYTIKKDLSQKERF
                     MKFIK"
     gene            2919..3362
                     /locus_tag="PLF66_21500"
     CDS             2919..3362
                     /locus_tag="PLF66_21500"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HMZ66678.1"
                     /translation="MSLLNEKAPDFTLPNSEGELIALSSFKGKKLLLVFYPGDETMVC
                     TKQLCSYSSGFEDFQKLGIDIIGINMDSVESHKKFKEKYKLAFPLLSDSSGSVCEKYN
                     AKGLLGVKRSTFLLDENQTIIFENEVLPVFYKDKDEVLTEIKKLA"
     gene            complement(3363..3788)
                     /locus_tag="PLF66_21505"
     CDS             complement(3363..3788)
                     /locus_tag="PLF66_21505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66679.1"
                     /translation="MSTMHNAMRFLTLLLLIFSIKPVLGLDTTIVLKDGEILKVDLID
                     ENDLTLIYKKKNDGVTGILAREEVLKIIYKDLNQDEISQIRAEEKIKIIEYRKLKKKK
                     KIENKKNYEMEMDDTEYEPAYIQDREEIPRDLAFLLRRK"
     gene            complement(3805..4293)
                     /gene="sixA"
                     /locus_tag="PLF66_21510"
     CDS             complement(3805..4293)
                     /gene="sixA"
                     /locus_tag="PLF66_21510"
                     /inference="COORDINATES: protein motif:HMM:TIGR00249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HMZ66680.1"
                     /translation="MKLIIGRHGEAESISFSGLDRDRQLTDKGRFDIEKMAKFIATSP
                     LKVNHIFHSPYKRTTETAEIFASKLSFQGTPIPADELLPSNNYSEILTHFSKFTNSDT
                     VLIIGHNPDVSFFASRLIKDESIARSFVFSTGTTIAINTPKENFAYGQLIWMISPDFL
                     KT"
     gene            4468..5382
                     /locus_tag="PLF66_21515"
     CDS             4468..5382
                     /locus_tag="PLF66_21515"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMZ66681.1"
                     /translation="MNDNPIHIEDLYKQYDSKSIAIKKLSLTIQPGEIFGLIGQNGAG
                     KTTLIKILLGFSSPTSGKVEVYGKKNSVEFKTKIGYLPEKVMIHPFLTAFEFLYLCGK
                     LCFIDKNILKKKCASILERVGLGDKLNAKVGTFSKGMTQRLGLAQAIIHEPDLLFLDE
                     PGSGLDPLGMIELRNIILEDHKSRNATIFINSHRLMEAEKLCSRIAILHKGEMVASGS
                     MNELTRTKNQINLRLEALTEKLENYIGSIGNQIQFKGNEISFDPKESIDLRQIPMQIV
                     QNGGNILKYEIVNENLEEIFLRLTGGEK"
     gene            5379..6206
                     /locus_tag="PLF66_21520"
     CDS             5379..6206
                     /locus_tag="PLF66_21520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66682.1"
                     /translation="MKIILSHIFSSIFTIAGLTIREAMRKRIIFVILAISSFFLLMNF
                     FCESVTMQANGEEVKDDAFGSVFIFIIVILWSFVISALVTSSLLADEFENKTYTMILS
                     KPIFRLSYLGGKVLGVFLLIILNAILIIGIYSIISVIRSKTIDSSLFMAAISMLPAYI
                     LLVSIVLLFTVLINRTASVLLSFAIVLLTSVINYPMYEAAFEKVIDTNSSKKMILEII
                     YWILPQFGTSLFHAISKVAKSFSQVHYLGLYSYFQIGIWILLTWILIYFSFRKRELE"
     gene            6203..6679
                     /locus_tag="PLF66_21525"
     CDS             6203..6679
                     /locus_tag="PLF66_21525"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004499264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HMZ66683.1"
                     /translation="MKPYRKNVGIVVFNSKGEVLVGRRVGPYRDNWQYPQGGIDEDEE
                     PKQAAVRELYEEVGIKEVQFVYEAPKWISYDFPKDLDIKLSKKYAGQIQKWFLVFWDK
                     SVSDCNLHTYEPEFEMVQFIPIEMTIDKVVPFKKHVYQEILNLFQPEIQKYLSSKR"
     gene            6679..7092
                     /locus_tag="PLF66_21530"
     CDS             6679..7092
                     /locus_tag="PLF66_21530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016546359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacitracin resistance protein BacA"
                     /protein_id="HMZ66684.1"
                     /translation="MEREIYTPPGGPPQDPINPNLFKNLGEEKIRSLANFFYKEIALS
                     AIRPMYPEDLKPGEEKLADFLIQVMGGPAYYSRKYGHPRMRARHFPFEIDEKARRVWL
                     SCFKKALDAIDVNENDKVDIYSFLTSFSAWMVNKA"
     gene            7190..7837
                     /locus_tag="PLF66_21535"
     CDS             7190..7837
                     /locus_tag="PLF66_21535"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HMZ66685.1"
                     /translation="MGFHIETFPVYPLGCNCSIVSCDESKEAIVIDPGGSEDQIFRYL
                     NSKNLRVTQIIHTHAHFDHCLGTKTIADANENCKIGLHKDDLKLYQNIHMQCQMFGIH
                     YSGDIRELDFYLEDNQILSIGKSTNLEVLHTPGHSPGSVCFLLRSSDKHIVFSGDTLF
                     AGSIGRTDLWGGDYDLILKSIQNRLFTLEDETLVIPGHGEGSVIYKEKLYNPYLN"
     gene            7891..8943
                     /locus_tag="PLF66_21540"
     CDS             7891..8943
                     /locus_tag="PLF66_21540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66686.1"
                     /translation="MMIIRLISYLLLLIMLCGTLAVSAVSWSFYESVYSSLNLLKYTS
                     DNKARDILATVARVAETKMYSDGYNEMNDFFVRMIKQSEKDLDKFTIKEVFLISPEGV
                     VLAHSNPSEITGEIISRTPSQKYNKPYYMRALRMRKGQLPTPQTFGQEYKGDETFFGR
                     MMMRLFPELKYQTVLLSAPIYHLEKLETVGSIHLIYNRGNVLFFMESQKEIFLWMLLN
                     YIGISFLVSIALWGTYVLFLFGSYKQGGRNTANIPEPELPKTLAKIETIFEKQEDTIK
                     RYLTPIPLKLDNTILNAESITEIKQPPVHPENQTTAKVPKVESSSLNGSNGNGKMANT
                     NNKANPTEALDAIYLD"
     gene            8943..9890
                     /locus_tag="PLF66_21545"
     CDS             8943..9890
                     /locus_tag="PLF66_21545"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HMZ66687.1"
                     /translation="MLDVSIKENHLSETQVVFVNIKGALDSEKAIDFYDFINAELIKG
                     FRKFVVDCSGLDYISSAGISIMIRLQHKLDQKKSILAYYALNKEITLVLNFFGLSKEF
                     PIADTAENALKLLDLHQEPFSGEEEIYSVHSEALHVTDNTSHTESLPIDTTTPRTESL
                     QVQVSEYKVSSLLDPETAKKIKTNRLTLTDEFPVLEEDESPTSQIEPIPPEEIKKESP
                     PFTELKIKLPFITRTETNPAKSTIMDSITDFETDHTERYDVGSLSNVTDTNFTVLVVN
                     CGNCGSKIRIRKQGKQKCPNCGYKFLLRQSGSISTIERL"
     gene            9896..12391
                     /locus_tag="PLF66_21550"
     CDS             9896..12391
                     /locus_tag="PLF66_21550"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HMZ66688.1"
                     /translation="MTEAELKSLNGLTRKEAEKRIKKFGLNELPSGKQKTILEIALAA
                     IKEPMFLILLSCSCIYFILGDIQEAIFLSISVLFVLGITIYQENKTENSLQSLKDLSS
                     PRALVIRDGKEEHIAGSLLVPDDLIIVKEGDRIPADAYVIWERNLSVDESILTGESFS
                     VRKFSGSEKDELGDKIYSGTLVVSGQAVASVFATGLLTEIGKIGVSLENTEETDSPIQ
                     NEINSLVKVFSIIGIGLCIVIAVVYGFTRGYWLKGFLAGLTLAMSILPEEFPVIYTVF
                     MALGAWRISKENVLARKRGAVEILGSANVLCVDKTGTLTQNKMSLQRIWSKGFFLDTN
                     SNGMDEIPENFHELLEYGILASKKDPFDPMEKELLRFGGSWLKNTEHLHPDWNLIFEY
                     PLSKELLSLSHVWVSADTDEYIIGSKGAPESIWDLCHLSEEEKERLSVIVHDLASSGL
                     RILGVAKSTFKKNELPKSQHDFPFQFLGFLGFADPVRENVSNSIKECIQAGVKVVMIT
                     GDYEGTAKSIAEKIGLPLGKHIITGSELNLLSDAELRERIVDTSIFCRVAPEQKLRIV
                     NIWKELGRVVAMTGDGVNDAPALKSADIGIAMGKRGTDVARESSDLVLVDDDFSSIVK
                     SIRLGRRIFTNLKNAMTYALAIHIPIAGLSLFPVLFGFPLLLLPMHIVFLELMIDPAC
                     SIVFEAGKEAIDTMNKPPRMIIEKIFNPYLLKIAFLQGLSVLFFSILSFYIFWRQDHL
                     EDYSRTACFVTLISGNVFMIVKNLAGDKPRSILQSMNIPFRYLATSAFSLLFLIVYIP
                     FLYQLFHFSPLKYTDLFISFGLGLLSMLVLLFF"
     gene            complement(12581..13009)
                     /locus_tag="PLF66_21555"
     CDS             complement(12581..13009)
                     /locus_tag="PLF66_21555"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HMZ66689.1"
                     /translation="MQLTNYTDYSLRVLIFLSIHSDRKVTIKEISSTYKISKNHLVKV
                     VHKLSTLGYLNTTSGVKGGITLAMTPNKINLADIIKKIEPSFHIVECFNPQGDCVIHP
                     VCELKSILNKALQAFLKSIDQYTLADISKKQSAYRKFLTI"
BASE COUNT         4471 a         1908 c         2369 g         4447 t
ORIGIN      
        1 aaattaacat cggctagata gagaaatcat tattataatg aatcgaatga ttggatttta
       61 gaagatttga aataatcatc tgcgtggatg gattgaaagg gttaaaaatg cttaccaaaa
      121 aaagttatgt caatttttct aactatttta tatcttccaa acaagctata gtttaaaatg
      181 cactttctcc aatctatgat tttaaaaaca aacccttatg atttgccttt ttttaaaata
      241 gatcaggatc gcaagactat ctgaattctc tctgcgaatt tgtttacaaa tagagaaaca
      301 ttcgaaattc aattttcaaa ttcttctcaa aaataagtgt gttgaaatag tttttaagtg
      361 aaaaatgatt cttgaaatgt tgaataaaaa aactagctta agtaaaacgt tattagagat
      421 attttcaaaa cagagtcaat attgctgttt ctatagatta tgcattttaa tctgcatttt
      481 gaaaaattat aaaaaagaga aatgaaataa tataagtttt tgatcctatt ttcccttact
      541 ttctaaactt tttttataac agtttataat tttataaaat ttaaatttag tatattagta
      601 aactctaatg cacttttgat ttacgattaa atatctaaaa aagaacaaaa aaagaacaga
      661 atatccgttg tatctatttg aattttttct aaaatctaac ttgcagtttc attcaatact
      721 ttgaatgaat tctaacgata cattgcaatt catcatttct tggatggaaa gtagagtata
      781 aatttagaat tacccaatta aattctaaat tcaattttaa ggatttagaa ttgattcatt
      841 caaataggca ttctttggtg aaaaaaaaat aaaaaaaagg agctaaaaaa aactgttcgg
      901 catatttttt atttttttag aataaaatta tgtctctcga aaaaataatt ctcatttctg
      961 aaaaaaactt aattaaggat tgaccttgac ctacctattt ccgatataat attcaggaaa
     1021 atacactcaa atgtattttc taaaaaatat tggaagagag actcaaaggt ataaacaatg
     1081 aaaattatca aaggtatatt tattctttta gtgttggtta atttaaacct cttgcttgca
     1141 caaaaaactg aaacagctcc tgctggtggt gcaaaggatg ctgcagagcc gaatgaagct
     1201 ggggatacaa tcaagggtga attaattgcg gatgacgata cagatgctgg gttatcggat
     1261 gaagagagaa aaaagaaaaa agaaactctg aagcatgaag ctatgtggaa gaatcaagac
     1321 tttgttggct acaataaagc attcgaagaa ttacataaat tatcaaaagc atttgcaaac
     1381 aataaataca gacttgctct ttctgcttac caatcaggaa tgaacacaat tctcaaaatg
     1441 agagaaagtg ttgagcaata tagaaaagaa caagctgaga aaaagagatt agatgagaaa
     1501 tggtactggc aaaaaatcga tcgtaaagct aacgaagaaa gatacattgc aagagaaaaa
     1561 atggctgcta aactagctgc tgttacctat ttcacaaaag caatcaacca tctcgatgat
     1621 attacaaatc ctgacttgag agaaactgat gcttacaaaa ctcttctttc ttccgtttac
     1681 agatcttgga ttatggcaga gtacgataca caaaactacc cacagtgtat cccaatcctc
     1741 gagctctatc ttgaaatcaa agaaaacgag aaagaatatc cagctcataa gtatttagca
     1801 agctgctact ctttcgaaga aaacactttg agaaaatata aagcaggaac tgacgatcaa
     1861 atccaaaaat taaagtataa gaaaaacgtt catcttttaa gagctactga attaaagtat
     1921 ggaaaaggta ctgtagagta caaacacatt gttgatttag taaataaaga tgaagtgatt
     1981 tctgttaatc cttaatctaa ttccgctctt tcaattaaaa taaaactatc cttaacagga
     2041 tggttttatt ttattgaaat taatggaaaa ggatacaatt ctatacgttc ttacaaaatc
     2101 ccaagaatac cttggcaaaa aacaaatccc aaatcccaga ctagacgctg aactaattct
     2161 ttccgatatt ctaaacctgg aaagaattaa gctttactcc aattttgata aaaaactaac
     2221 cgaatcagaa aaagatatat atcgagaaag aataaaagct cgaggtgagt ttaagcctgt
     2281 tgcttatata acaggttata agcattttta taaaagtatt tttaaagtga atgatcaagt
     2341 tctaattcca cgacccgaga cagaagaact aattgagtgg atttcaaaag agcaaaatct
     2401 aaatcaaaaa gtttccctgt tagatctttg tactgggagt ggttgtatag gaattagtat
     2461 taaaaaagac tttcctgccg ttgaaatgac ttttagtgat ttatccgaac aggcaatttt
     2521 aattgcagaa gaaaatgcta aaagtatact tggggaagat aaagccaaat tcattcaaag
     2581 tgatttattt gattctatca aagattcctt tgattttatt gtctccaatc ctccgtatat
     2641 tccaattgaa gaaaaagaaa ttttaatgcc tgatgttgta aattatgaac cacacctcgc
     2701 ccttttttta gaggatccaa atcattttta caatagattt ttaatcggtg catttgagca
     2761 tttgaatcca aatggagttt tttattttga gactcatcaa gattgggcaa gtaaaattgg
     2821 aaaagtcggt ttggagatag gctttaaaga ctatacgata aaaaaagatt tgagtcaaaa
     2881 agaacgtttt atgaaattta taaagtgagg ttttacgaat gagtttatta aatgaaaaag
     2941 ctcctgattt tacattgcct aattcagaag gggaactgat tgcactgagt tcgtttaagg
     3001 gtaagaagct attacttgtt ttctatccag gtgatgagac aatggtgtgc acaaagcaat
     3061 tatgctctta tagtagtggc tttgaggatt ttcaaaaact tggaattgat atcataggaa
     3121 taaacatgga ttctgttgag tctcataaga agtttaaaga aaaatacaaa ctagcttttc
     3181 ctctattatc tgattcttca gggtccgttt gcgaaaaata caatgcaaaa gggttattag
     3241 gagtgaaacg ttcaacattt ttattggatg aaaatcagac tattattttt gaaaatgaag
     3301 tattgcctgt tttctataaa gataaagatg aggttttaac tgaaataaaa aaattagctt
     3361 aattatttac gtctgagaag aaatgctaag tctcttggaa tttcttctct gtcttgtata
     3421 tatgcaggtt catattcagt atcatccatt tccatttcgt agtttttctt gttctcaatc
     3481 ttctttttct tttttagttt acggtattcg atgatcttta ttttttcttc tgctctaatt
     3541 tgggaaattt cgtcttgatt caaatccttg tagataatct tcaatacttc ttctctggct
     3601 aggattcctg ttacaccatc atttttcttt ttatagataa gagttaaatc gttctcatca
     3661 attaaatcta cttttaagat ctcgccatcc tttaatacga tggtagtgtc taagcccaaa
     3721 acaggtttga ttgaaaaaat taggagtaaa agggttaaaa atcgcatggc attgtgcatt
     3781 gtactcatgg taaatttttg tcaattaggt tttcaaaaaa tctggtgaaa tcatccaaat
     3841 taactgacca tacgcgaaat tttcttttgg tgtattgatc gcaatggttg ttccggttga
     3901 aaaaacaaag cttctagcta ttgactcatc ttttattaga cgggatgcaa agaaacttac
     3961 atctggatta tgcccaatta taagaactgt atctgaattt gtaaattttg agaaatgggt
     4021 tagaatttca ctgtaattat tggaaggtaa taattcgtct gcgggaattg gtgttccttg
     4081 gaatgaaagt ttggatgcaa atatttctgc ggtttcggtc gttcttttat aggggctatg
     4141 aaaaatatga ttcactttaa gaggactcgt tgcaatgaac tttgccattt tctcaatatc
     4201 gaatctgccc ttatctgtta attgccgatc tctatccaat cctgaaaaag aaattgattc
     4261 agcttcacca tgtcttccaa tgattaactt catataaatc ctgtgtagac taatatcctg
     4321 ttctgccttt tcatttttac attttttttg aaaaatataa gcagctattc tatataaaaa
     4381 cttttaacag attttgcccg tttagtcttt ctatatatcc tatttttcac tagccattcc
     4441 tttgtaatca gaaaaatttg ttttgtaatg aatgacaatc ctattcacat tgaagattta
     4501 tataagcaat atgactctaa gtcaattgca attaagaagc tttctctaac tatacagcct
     4561 ggtgaaatat ttgggttaat tggtcagaat ggcgccggaa aaactacatt gattaaaata
     4621 ctacttggtt tctcttctcc tacaagtgga aaagtagaag tctatggtaa aaaaaattcg
     4681 gtagagttta aaacaaaaat cggatatttg ccagaaaaag taatgatcca tcctttttta
     4741 actgcctttg agtttttata tttatgcggt aaactctgct ttattgataa gaatatttta
     4801 aaaaagaaat gtgcttctat tttagaaagg gtagggcttg gtgataagtt aaatgcaaaa
     4861 gtaggaacat tctcgaaggg aatgacgcaa aggctaggat tagcacaggc aattattcac
     4921 gaacctgatt tattattttt agatgaacca ggttcaggtc ttgatccact tgggatgatc
     4981 gaacttcgaa atattatact agaagaccac aagtctagaa atgctacaat ttttataaat
     5041 tctcatcgtt taatggaagc tgaaaagtta tgttctcgta ttgctatttt acataaaggt
     5101 gagatggttg cgagtggttc tatgaatgaa cttacacgaa caaaaaacca gattaattta
     5161 cgtttggaag cgttaaccga aaaattagaa aattatattg gatcaatagg aaatcaaatt
     5221 caatttaaag ggaatgaaat atcctttgac ccaaaggagt ctatcgattt gcgccaaata
     5281 ccgatgcaga ttgttcagaa tggtggaaat atcttaaagt atgaaatcgt gaatgaaaac
     5341 ttggaagaga tttttctcag attgactgga ggcgaaaaat gaaaataata ttaagccata
     5401 ttttctcgag tatttttact attgcaggtc ttacaatcag agaagcaatg agaaagcgaa
     5461 ttatttttgt gattttggct atatctagtt tttttctttt gatgaatttc ttttgcgagt
     5521 ctgttacaat gcaagcgaat ggagaagaag taaaagatga tgcatttggg tctgtattta
     5581 tttttattat tgtgatattg tggagttttg ttatttcagc actcgttaca tcctctttac
     5641 tagccgatga gtttgaaaac aaaacatata caatgatttt atctaagcca atttttcgac
     5701 tgagttattt aggaggaaaa gttttaggtg tatttttatt aatcatttta aatgcaattt
     5761 taattattgg aatatacagt attataagcg ttattcgctc taagactatt gattcaagtt
     5821 tatttatggc tgcaatttca atgcttcctg cctatatttt acttgttagt attgttttac
     5881 tttttacggt attgattaat cgaaccgcat cagtgttatt atcatttgcg attgttttgc
     5941 ttaccagtgt gattaattat ccaatgtatg aagcagcctt tgaaaaagta atcgatacaa
     6001 actcatccaa aaaaatgatt cttgaaatta tctactggat attaccacaa tttggaacta
     6061 gtttatttca cgccatttct aaggtagcaa aatccttctc acaagttcat tatttaggtc
     6121 tttattctta ttttcaaatt ggaatctgga ttttactaac ctggatacta atctatttta
     6181 gttttagaaa aagagaatta gaatgaaacc ttataggaaa aatgttggga ttgttgtctt
     6241 taattcaaaa ggcgaagttt tagtaggaag gcgagttgga ccctatcggg ataattggca
     6301 gtatccgcaa ggcggcattg atgaggatga ggaaccgaaa caagcggctg ttagagaatt
     6361 gtatgaagag gttggaatta aagaggttca attcgtatat gaagcaccaa aatggattag
     6421 ttatgatttc ccaaaagatc tggatataaa attaagtaaa aagtatgcag gtcaaattca
     6481 aaaatggttt ttagtatttt gggataaatc cgtgagtgat tgtaatttac atacttacga
     6541 gccagagttt gaaatggttc aatttattcc aatcgaaatg actattgata aagtcgttcc
     6601 ttttaaaaag catgtgtatc aagaaattct aaatttattt caacctgaaa ttcaaaaata
     6661 tttgagtagt aaaagataat ggaaagagaa atttataccc cacctggtgg acctccacaa
     6721 gatccaataa atccaaattt atttaaaaat ttgggagaag aaaagataag aagtctagct
     6781 aatttttttt ataaggagat tgcactctct gcgattcgtc ctatgtatcc agaggacttg
     6841 aaacctggtg aagaaaagtt agctgatttt ttgattcaag tcatgggtgg tccagcttac
     6901 tattcaagaa agtatggaca tccaagaatg agagctaggc atttcccttt tgaaatagat
     6961 gagaaagcga gaagagtctg gctttcttgt tttaaaaaag cgttagatgc catagatgta
     7021 aatgaaaacg ataaggtgga tatatattct tttctgacta gcttttctgc ttggatggtc
     7081 aataaagctt aactatttca aatgaatttt tagatgaagt cctttgcttg tatctaaatt
     7141 actgcttttt atttctttac tttttattct tatagaaatt ctatgaagta tgggttttca
     7201 tattgaaaca tttcctgttt atccactcgg ttgcaactgt tccattgtgt cttgtgatga
     7261 atcgaaagaa gcaatcgtga ttgatccagg cggaagtgag gatcaaattt ttagatattt
     7321 gaactcaaag aatttaagag ttacacagat tattcacact catgcccact ttgatcattg
     7381 tttaggaaca aagaccattg cggatgcgaa tgaaaactgc aaaattggat tacataaaga
     7441 tgatttgaaa ttgtatcaaa atatccatat gcagtgtcaa atgttcggta ttcattattc
     7501 aggtgacata agagagttag atttttattt agaagacaat caaattttat ctattggaaa
     7561 atcgaccaat cttgaagtct tgcatacacc tggtcatagt cctggatctg tctgctttct
     7621 tttaaggagt tccgacaaac acatagtctt ttcgggtgat acgctttttg cgggaagcat
     7681 tgggcgcact gatctttggg gtggtgatta cgatttaatt ttaaaatcta ttcagaacag
     7741 attatttaca ttagaagatg aaacgcttgt cattccaggt catggtgaag gtagtgttat
     7801 ttacaaggaa aaattataca atccatatct aaattgatgg cttaggtaat aaaacatgca
     7861 cataattccg acaattgtag gagaacgttt atgatgatca ttcggcttat atcctattta
     7921 ttactgctta taatgttatg tggaacactt gcagtttcag ctgtatcgtg gtctttttat
     7981 gagtcagttt attcttcttt aaaccttctc aaatatacat ctgataataa agctcgtgat
     8041 attttggcaa ctgttgctcg tgtagcggaa accaaaatgt attcagatgg ctacaatgaa
     8101 atgaatgatt ttttcgttcg aatgattaaa cagtcagaga aagatttaga taaatttaca
     8161 attaaagaag ttttcctaat ctcaccagaa ggtgttgttc ttgcacacag taatccatca
     8221 gaaataacgg gagaaattat ttctcgcact ccttctcaaa agtataataa accctattac
     8281 atgagagcac ttcgaatgag aaagggacaa ctaccaactc ctcaaacatt tggacaggag
     8341 tataaagggg atgaaacatt tttcggtaga atgatgatgc gtttatttcc agaattaaaa
     8401 taccaaacag ttctcttatc tgcaccaatt tatcatttag aaaaattaga aacagtaggt
     8461 tccatacatt taatttacaa tagagggaat gttctctttt ttatggaaag ccaaaaggaa
     8521 attttccttt ggatgctctt aaattatatt ggaatatcat ttttagtaag tattgcgttg
     8581 tggggaacat atgttttatt tttattcggt agttataagc aaggtgggcg taataccgct
     8641 aatatacctg agcccgaatt gcctaagaca cttgcaaaaa ttgaaaccat tttcgaaaag
     8701 caagaagata caattaagcg ttatttgaca cctataccac taaagcttga taatacaatt
     8761 ctgaatgcag agagtattac agagattaaa caacctcctg ttcatcctga gaatcaaaca
     8821 acagccaaag ttccgaaagt ggaaagttct tccttaaatg gtagtaacgg aaatgggaaa
     8881 atggctaata caaataataa agcaaatcca acggaagcct tggatgcaat ctatcttgac
     8941 tgatgctaga tgtttcgatt aaagaaaacc acttaagtga aacgcaagtt gtatttgtaa
     9001 atataaaagg tgcgttagac tccgagaaag ctattgattt ttatgatttt atcaatgccg
     9061 aattgataaa aggcttcaga aagtttgtag tggattgttc aggtttagat tacatatctt
     9121 cagctggaat atcgataatg attcgactcc agcacaaatt agatcagaaa aaatctatat
     9181 tagcttatta tgctttgaat aaggaaatta ctcttgtcct aaattttttt ggtttatcaa
     9241 aagaatttcc cattgcggat acagcagaga atgcactgaa acttttagat ttacatcagg
     9301 aacctttctc tggagaagag gagatttact cagtgcattc tgaagcattg catgttacag
     9361 ataatacaag ccatacagag tctttaccta ttgatacaac aacgcctaga acagaatctt
     9421 tgcaagtgca agtttctgag tataaagtat cctctctttt agatcctgaa acagcaaaga
     9481 aaatcaagac aaatcgccta acgctcactg atgaatttcc tgtgctcgaa gaagatgaat
     9541 ctcctacctc acagattgaa cctatccctc cagaagagat taaaaaagaa agtccgcctt
     9601 ttactgaatt aaaaataaag ttaccgttta taacaagaac agaaacaaat cctgcgaaat
     9661 ctacgattat ggatagtata acagattttg aaaccgatca tacggaacgt tatgatgttg
     9721 gatcattatc aaatgttaca gatactaatt ttactgtgct tgttgtaaat tgtggaaact
     9781 gtggttctaa aattcgaatt cgaaaacagg gaaaacaaaa atgtccaaat tgcggttaca
     9841 aatttttatt gagacaatcg gggtctattt ccacaatcga gaggctgtag cttgaatgac
     9901 agaagcagaa cttaaatctc taaacggtct gacaagaaaa gaagccgaga agaggataaa
     9961 aaagttcggc ttaaatgaac ttccatcagg aaaacaaaaa acaattctag aaattgcatt
    10021 ggctgctatt aaagaaccaa tgtttttaat actactgagt tgtagttgta tatatttcat
    10081 tttaggtgat attcaagaag ctatctttct ttcaatatcc gtattatttg tattgggaat
    10141 tacgatctat caggaaaaca aaacagagaa ttctcttcag tctctaaaag atttatcaag
    10201 tcctagagcg ttagtaataa gagatggtaa ggaggagcat attgctggta gcctgcttgt
    10261 tccagacgac cttattatag ttaaggaagg ggataggatt cccgcggatg cctatgttat
    10321 atgggagaga aatttaagtg tggatgagtc tatacttact ggagagtcat tttcagttag
    10381 aaaattttcc ggaagcgaga aggacgaatt aggtgataag atctattctg gaacgttagt
    10441 tgtctcaggt caagctgttg cttctgtttt tgcaacgggc ttgctgacag agattggaaa
    10501 aattggagta tcgcttgaga atacagagga aacagacagt cctatacaaa atgaaatcaa
    10561 ctctcttgta aaagtttttt caattattgg aattggttta tgcatagtga tagcagttgt
    10621 atatggattt acgcgtgggt attggttaaa gggttttttg gcaggactta cacttgctat
    10681 gtctatctta ccagaagagt ttccagttat ctatacagta tttatggcgt taggtgcatg
    10741 gcgcatttcc aaagaaaatg ttctcgctag aaaacgaggg gcagttgaaa tcttaggttc
    10801 agcaaatgtt ttgtgtgttg ataagacagg aaccttaaca caaaataaaa tgagcttgca
    10861 aagaatctgg tcgaaaggct tttttcttga tactaattca aatggaatgg atgaaattcc
    10921 tgaaaatttt catgaattac tagaatacgg gattcttgca tcgaaaaaag atccatttga
    10981 tcccatggag aaagaacttt taagattcgg aggaagctgg cttaaaaata cagagcattt
    11041 gcatcccgat tggaatctta tttttgagta tcctctttca aaggaattac tttcattatc
    11101 tcatgtttgg gtttcagcgg atacggatga gtatataatt ggatccaaag gagcgccaga
    11161 gtcaatttgg gatttatgcc atttatctga agaagagaaa gagcgcctat cggttattgt
    11221 gcatgatcta gcttctagtg gattgcgtat attaggtgtt gctaaatcta catttaagaa
    11281 aaatgaatta cctaagagtc aacatgattt tccatttcag tttttaggat tccttggttt
    11341 tgctgatcca gtgcgagaga atgtttccaa ttcaattaaa gaatgtattc aagctggtgt
    11401 aaaagtagtt atgatcactg gtgattatga aggaacagcg aaaagtattg cagagaaaat
    11461 aggtctgcct cttggaaagc atatcattac aggatctgaa ttaaatttat tatctgatgc
    11521 agaattaaga gaaagaatag tcgatacgag tatattttgc agggttgctc ctgaacaaaa
    11581 acttagaatt gtgaatatat ggaaagaatt aggtagagtt gttgctatga cgggtgacgg
    11641 tgtaaatgat gctccggctc tcaaatctgc agatataggt attgcgatgg gaaaaagggg
    11701 aaccgatgtt gcaagagaat cttcagactt ggttcttgtt gacgatgact tttcttccat
    11761 tgtaaaatcg attcgtcttg ggcgtagaat ttttacaaat ttaaaaaatg ctatgaccta
    11821 tgcacttgct attcatattc caattgctgg attgtctctt tttcctgtgc tctttggttt
    11881 cccattactt cttttgccga tgcatatcgt ttttttggag cttatgattg atccagcttg
    11941 ttctattgtt tttgaagctg gaaaggaagc gatagacaca atgaacaagc cacctcgtat
    12001 gataatagag aaaatattta atccatattt acttaaaatt gcttttctac agggtttgag
    12061 tgttcttttc ttttcaatat tgtcttttta tattttttgg cgtcaggatc atttagaaga
    12121 ttattctaga acggcttgtt ttgtgactct aatttcgggt aatgtattta tgatcgtaaa
    12181 gaatttagca ggagataaac ctcgaagtat tctacagagc atgaatatac catttcgata
    12241 tcttgcgaca agtgcattta gcttattatt tctaattgtt tatatacctt ttttatacca
    12301 gttattccat ttttctcctt tgaaatatac tgatttattt atttcctttg gtttaggtct
    12361 tttatctatg cttgtcttat tatttttcta aaaaatattg tagtggctga ttcgccacta
    12421 caataaaagt aaaatggaaa aagattctaa tttttatatt gtaaattgaa atgaaaaaaa
    12481 tacgagtgta aattttagat gaatacaata gaaagtatga tttatatcat acgaatttaa
    12541 atctttaatt gttaagtaga aattctagat agcactagtt ctatattgtt aaaaatttgc
    12601 gatatgcgct ttgcttcttt gatatatcgg caagagtata ttgatcaatc gattttagga
    12661 aagcctgtaa tgctttattg agaattgatt tgagttcaca aacaggatgt ataacacaat
    12721 ctccttgagg attgaagcat tcaacaatat gaaagctagg ctcgattttt tttatgatat
    12781 ctgctagatt tattttattg ggagtcattg ctagggtaat tccacctttc acgccgctag
    12841 ttgtattcaa atatcctagt gtagataatt tatgaacaac ttttactaag tggtttttag
    12901 agattttata agtagatgaa atttctttga ttgttacttt tctatcagag tgaatagaca
    12961 gaaaaattaa aactctaaga gagtagtctg tatagtttgt taattgcata atttcacatg
    13021 aaaattgact tagaattatt tcaaatattt tttcctttac aattattgta ttctaaatat
    13081 attaattact cacttcaata tagatagagg aattagcttc tggatgtagt atataagaat
    13141 attcgtaacc tatcaggctg ctaagttact tttatcaaga gaatataatt ttaat
//
LOCUS       DAOQOS010000136        13074 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_2449_length_13074_cov_7.065212, whole genome shotgun sequence.
ACCESSION   DAOQOS010000136 DAOQOS010000000
VERSION     DAOQOS010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 13074)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13074)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13074
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2449_length_13074_cov_7.065212"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..198)
                     /locus_tag="PLF66_21560"
     CDS             complement(<1..198)
                     /locus_tag="PLF66_21560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66690.1"
                     /translation="MADVKDKIYSQYLSEQKRYKRFVPILRYFGYSMLILYLILLFTQ
                     KGLPPKFFLLNIYSIYCNMSLF"
     gene            305..1237
                     /locus_tag="PLF66_21565"
     CDS             305..1237
                     /locus_tag="PLF66_21565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011172425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-NADP reductase"
                     /protein_id="HMZ66691.1"
                     /translation="MKPVREPKINLFKKSAPLKAKVLKNIQLTPDPGKGKRPKKEGMA
                     SIHRIVIGIDHTQFPYVIGQSAGIIPPGKDPEKVAKGADNTDYAIRLYSISSPTYSMG
                     MKEDSIEFIIKRDNAYDEEGNILFSGVGSNYMCNLKEGDEVTMTGPAGKNFLLPTSDF
                     SGDIYFCATGTGIAPFYGMTYELLEHKLINFTGRITIIYGAPYSDEIVLLDEFLELEK
                     KHKNFKLITAISREQKNSFDGGRMYIGHRVRELAGQIKESFANGGRLYICGGPKGMEK
                     GVIQELQSALGDNGDLAHFEEKLKEQKQLFVETY"
     gene            1237..1455
                     /locus_tag="PLF66_21570"
     CDS             1237..1455
                     /locus_tag="PLF66_21570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004423064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional coactivator p15/PC4 family
                     protein"
                     /protein_id="HMZ66692.1"
                     /translation="MGENGIIRDIDKGKGEIIRVEISEFKGKKLLNLRVWYTDANGEY
                     KPTQKGIAIPPDLYEQVRDAINEAGSRF"
     gene            1576..3522
                     /locus_tag="PLF66_21575"
     CDS             1576..3522
                     /locus_tag="PLF66_21575"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HMZ66693.1"
                     /translation="MINPRSRLFSFYFLFFVFVFLSYRIFFLYLYQDKLDNASIGLIL
                     KSILVGFRFDFSVIAAVLSPFYFLSLIFPLNRYKLYQFIWGFTPIILSIWVAFHLIGD
                     IVYFANSNKHLGYEGFIFLNKDIILLIKSFLLEKPFIFFVSIAISIFYLTSSFFLWVR
                     HFHFVETSRKIMNRLAELILILVLSVFLIRGGIQTTSLRPSHSIISDNNFINTLCLNG
                     IFTSFSDMIQTQIPNSKKIPIEEAILHVRESIEYEGAEFVNPAYPILRKSLKANSDKP
                     PSIVLILLESWTGKFVKPISKDGLIDGKEVTPNFNKLIQEGVFFKRFFATGGRTSNGL
                     FASLTGLPDRPMISVLHTQEANARVSGLGKILKQIDYQNLFMTGSSLAFENISPHIQR
                     WGFDRIIDERNIESTNKFKKGVWGYDDADGLSLFNEYLRELNPDKPFLGIYLTISTHY
                     PYKVPDPKFEIFDSKTMDFDFLNTYHYADYAIGKFIEEAKKEKYFSNTIFIFVADHTH
                     HRFLNHYEDRNIPFLIYAPNRFKPEIRNTISSQLDIIPTIVGLTGKPLLFSAMGKDLF
                     ASNIKSFAYFCFGYLYGWIDEEHFYFQNIGISTKTLNFPMTEPYIDRNNCDILPECKL
                     TERKTNAFLNLPIQLLERNLIFPY"
     gene            complement(3505..4410)
                     /locus_tag="PLF66_21580"
     CDS             complement(3505..4410)
                     /locus_tag="PLF66_21580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66694.1"
                     /translation="MNKILIILLFIFLHCGKDSIRGNIPIDYILLHEKILINEKEREF
                     ILFKPKEIKSNIILIGLHGRGGTGEEFMWKTEFNRLAEEQGFIIVYPSGLNRAWIDGR
                     KLPEEDPDANDETFLLTLREHLIKQFGNKKFFLFGYSNGGFMTQRMAFKYPEAFVGYA
                     SVVSTMSDVLSKEKPKQPVPVLFINGTNDPIVPYRGGLISGNKGIAVGAEETVRNWVK
                     WNRCNIKPDAEIVNELDDGVKVFSHSYSNCKSGKPVRFLKLEGAGHGWPGKIEEEKPG
                     FGKFTAEISANLEIWKFVDSLVRKN"
     gene            complement(4509..5162)
                     /locus_tag="PLF66_21585"
     CDS             complement(4509..5162)
                     /locus_tag="PLF66_21585"
                     /inference="COORDINATES: protein motif:HMM:NF021141.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2459 domain-containing protein"
                     /protein_id="HMZ66695.1"
                     /translation="MLRIGFILFLISCSSFKTWNYLPDTKEEETFIYVVSHGWHTGII
                     LAQENLGKDFEFLNSNFGSNPFYEFGWGDKGFYEAEEITTKITLKAMFLPTESVLHVV
                     AIPKDPKLYFNGSEVIKLPISKKAHVILNQAILNTFKRSNSGEIIKTRVGIYGNSFFF
                     LANGYYYITNTCNTWTAKTLEEAGLPFDRFFTLTARSVRSQAWDALAKYNCCQKGSD"
     gene            5534..7420
                     /gene="mnmG"
                     /locus_tag="PLF66_21590"
     CDS             5534..7420
                     /gene="mnmG"
                     /locus_tag="PLF66_21590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010679087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HMZ66696.1"
                     /translation="MDSFFPNRFDCIVVGAGHAGSEASFITARGGLRTLLITMNMDTI
                     GQMSCNPAIGGIAKGHMVREVDALGGLMGRVIDATGIQFKMLNTSKGPSVWAPRAQAD
                     KKEYQLKVKHTLEKEKLLSIRQDTVEDVIIEDNRVVGVKTGRGFEIFTNHLILTTGTF
                     LQSVIHIGKYQKESGRLGDGTVYGLSESLRHVNFRLGRLKTGTPPRLNKNSIDYSELA
                     IQDGDEDPTPFSFSNDAITRRQIPCHITYTNSETHRIINENLELSPMYSGQIKSTGPR
                     YCPSIEDKIVRFAERERHQIFLEPEGYETMEVYVNGTSTSLPEEVQWKLIRSIKGLEK
                     AEIMRPGYAVEYDFVDPTELNPTLETKKIKGLYHAGQINGTTGYEEAAAQGVVAAYNV
                     ILSFRKEQPMIFSRSESYIGVLVDDLVYKGVEDPYRMFTSRAEHRLLLRQDNADKRLM
                     KYGLQLGVVSKDDYHRMEEKYNRIDSLRQKIYGMNLKPSDALTELLKAKKIEAIKFGT
                     DMDSFIRRPEISLSDCKEIIPELNTISDKEIKVLEMEIKYEGYIKREQQMITQRNKSL
                     SVKIPEDFDYSQLTALKAEAIVKLTKHKPINLEKASQISGVDPSDIDILLFHLTDKAR
                     QEGK"
     gene            complement(7526..8449)
                     /locus_tag="PLF66_21595"
     CDS             complement(7526..8449)
                     /locus_tag="PLF66_21595"
                     /inference="COORDINATES: protein motif:HMM:NF014305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66697.1"
                     /translation="MKKTIYILLFLILLISCDRRLRASKEFSNSLDEIKFDWNGEERR
                     ALIYDPKFQKAQPLLLVLHGGGGKPENSVGIDGEYFLKAAKENGFIVVYPQGNDKKWN
                     DFREDPTKLDVKYRDDVGFIRELIQRISKSTLIDENKIFLTGISNGGMMSFRLACEMS
                     KTFKEVFPITANMPLNADTLCKPQNSVSITVFNGTEDPLVPYKGGTVKVLGKAHGEVD
                     STDATLKFWADKAGCANKESAELPKKYSNDPTSVIHHRWECPANRIELYEIRGGGHTW
                     PGGTKFLPEMMVGITTKEIEASKIIVNRILE"
     gene            complement(8614..8901)
                     /locus_tag="PLF66_21600"
     CDS             complement(8614..8901)
                     /locus_tag="PLF66_21600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012476361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-hand domain-containing protein"
                     /protein_id="HMZ66698.1"
                     /translation="MNNTLKLFLVVVISVSISLYADDEKEKGHHGHGKKHFKKMDTNG
                     DGKISKEEWVKFHETRFTEIDKDGDGSITDDEFKNFHKDKEEAKDSKRTKK"
     gene            complement(9280..10824)
                     /locus_tag="PLF66_21605"
     CDS             complement(9280..10824)
                     /locus_tag="PLF66_21605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HMZ66699.1"
                     /translation="MIKIILLLLTILLNTTLFAREVTFLPAYVIGEEPPALKKKDNVS
                     SGISELIAYYANENFLIEVSDPDKVKNYVRDSGETMDRKPSKSLLNGVCEEFGSDYIV
                     KTEINFDSSISILSEVYNCRGKVIGSNESLFKENFYSSIEKHTKRTLNFLVPKNKSDS
                     AYDLSGEEEIIFLFDMSGSLSREVKGIFRYILSITGSKNLSLGAVFVSESSIKILKPS
                     LNHDKLKEELGRIKQGGDVGIDRITQGLVKIRSELGLNRITKKKMIIVTDAKSSGTGD
                     YGYLSAVQAIKELGYNTFIVSGSFFDYKSMSLHKKAAKLGGNTLQQITHFQKVGTTKG
                     YKTIYLNDRTIYYDESEQVNPREIDLKEMVALDEGRILSKVDFPHPDNMSDVFERVQG
                     TRLIERQSISSNADSVVEKIVQSKGSISFIGSKILIKTGHTSFWITLKNTSEDLENQD
                     IVIKASFVRDEFSANGFINIPAQTEIYKESVPKLLVLEPAQIRNFLKSNSVFTCFVQG
                     RVVEVK"
     gene            complement(10988..12514)
                     /gene="guaB"
                     /locus_tag="PLF66_21610"
     CDS             complement(10988..12514)
                     /gene="guaB"
                     /locus_tag="PLF66_21610"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004429403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HMZ66700.1"
                     /translation="MTSSIIKNQPVQDGLSGEQLFSAQIGLTYRDFLVLPGYIDFNPS
                     DVNLESKVSRNITIKRPLISSPMDTVTESAMAIALALQGGLGIIHYNNTITEQVNFVR
                     KVKRYENGFITDPIILGPENTIEDLDDIKEKFGFSGIPITEDGTFNGKLIGIVTNRDI
                     DFEPNRKIKLSSVMTKTLVTAPVGISLKEANNILRVKKVGKLPIIDQDGKLVALVSRS
                     DLKKNKDFPDSSKDHNKRLLVGAAVSTLPESRDRVAALYDAGVDLIVIDSAQGNSSYQ
                     IEMIKFIKANFKNLDVVAGNVVTKEQSRSLIEAGADGLRVGMGPGSICITQETMAVGR
                     AQATAVYMTGSYAAQFGIPVIADGGISNIGDIANALAIGASTCMMGSMFAGTNEAPGE
                     YFYENGIRLKKYRGMASLEAMKAGGDKRYFSESQKIKVAQGVSGAVVDKGSVINFVPY
                     LVQGLRQSFQDMGHKTIPDLHAALYNGTLRFERRSESAQAQGSVHGLYSYTSPTMRVE
                     "
     gene            12589..>13074
                     /locus_tag="PLF66_21615"
     CDS             12589..>13074
                     /locus_tag="PLF66_21615"
                     /inference="COORDINATES: protein motif:HMM:NF019245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1577 domain-containing protein"
                     /protein_id="HMZ66701.1"
                     /translation="MEIIDRKIRETVDIKDAHKRVVILAKHLSKTEMQIKDTETHLKL
                     LEHNADGTKIIVERLDPTYILRKGQNLILTKLLGRYVQLDCTILGEKPGNLFLLQINK
                     FLVSSKERLNDRIHPPPEQVWVTNIRTSGVSIETNMHTIPTYMKINFTDYENKLRKNF
                     DY"
BASE COUNT         4324 a         2408 c         2043 g         4299 t
ORIGIN      
        1 aaataaggac atattacagt agatagaata tatattgagt agaaaaaact tagggggcaa
       61 acccttttga gtaaaaagca aaattaaata caaaataagc atgctatacc cgaaataacg
      121 aaggataggc acaaaacgtt tatatctttt ctgttcggag aggtattggc tgtaaatttt
      181 atctttcaca tctgccataa ttcgtcctat tatttaaact gcaaggatca aaggatatag
      241 ttttttcttg accctaggtt tttaaactaa aatctagtag taagttttca aaaaaggaac
      301 tgatatgaaa ccagtcagag agcctaaaat aaatctcttt aaaaaatcgg caccacttaa
      361 agcaaaagtt ttaaaaaata ttcagctaac ccctgatccc ggaaaaggga aacgtcccaa
      421 aaaagaagga atggcttcca ttcatagaat tgtgattggt atcgatcaca ctcagttccc
      481 atacgttatt ggacagagtg ccggcattat ccctccagga aaagatccag aaaaagttgc
      541 aaagggtgca gataacaccg attatgcaat tcgtctttat tcaatctcct ctcctaccta
      601 ctcaatggga atgaaagaag actccattga atttataatt aaaagagaca atgcatacga
      661 cgaagaaggc aatatacttt tctctggtgt agggtccaat tatatgtgta atttgaaaga
      721 aggcgacgag gttacaatga ctggtcctgc cgggaaaaac tttctactac cgacctctga
      781 cttcagcgga gatatttact tttgtgcaac aggaactgga atcgctccat tctatggaat
      841 gacttatgaa ttgttagagc ataagctgat taactttact ggaagaatta ctatcatata
      901 cggcgcacct tattcggatg agattgtctt attagatgaa tttttagaac tagagaagaa
      961 acacaaaaac tttaaactta taacagcaat tagccgcgaa caaaaaaatt cttttgacgg
     1021 tggacgaatg tatattggtc atagagtaag agaacttgca gggcaaatta aagaatcctt
     1081 tgctaatggc ggtagactct atatatgcgg tggtcctaaa ggtatggaga aaggagtgat
     1141 tcaagaacta caatccgccc ttggggataa cggtgattta gctcattttg aagaaaaact
     1201 aaaagaacaa aaacaattat ttgtggagac atactaatgg gagaaaatgg aatcattcgt
     1261 gatatagaca aagggaaagg ggaaattatt cgggtagaga ttagtgaatt taaaggtaag
     1321 aaacttttaa atctaagagt ttggtataca gatgcaaacg gagaatataa accaactcag
     1381 aaaggaattg ctatcccacc ggatctatac gaacaagtga gagatgcaat caatgaagca
     1441 ggaagtagat tctaatacta ttttctgcta ctctttgtcc atttaacctt tattagatac
     1501 aaattgattt ctaaattatt tgaattgggt agctcatttt ttaatagaaa ataaattttc
     1561 tctactgcta aattcatgat aaatccccgt agccgattat tttcttttta ttttttattc
     1621 tttgtatttg tttttttatc ttacaggatt ttcttccttt atctttatca agataaatta
     1681 gataatgcga gtattggttt aattctaaaa agtatccttg tcggatttcg ctttgacttt
     1741 tctgtaattg cagcggttct atctccattc tattttctct ctttaatatt tccattaaac
     1801 cgatacaagc tctatcaatt tatttggggg tttacaccga ttatcctctc tatttgggta
     1861 gcgttccact tgattggaga tattgtatac ttcgcaaatt caaataaaca tttaggctat
     1921 gagggattta tttttctaaa taaagatata attttgctaa taaaatcttt tcttttagag
     1981 aaacctttta ttttttttgt atcgattgct attagtatat tttatttaac aagttcattt
     2041 tttctgtggg tgcgtcattt tcattttgtc gaaacaagtc gaaaaataat gaatcgacta
     2101 gccgagctca ttctgattct agttctttct gtttttctga ttagaggtgg aattcaaact
     2161 acatccttac gcccaagtca ttcgattata tcagataata actttataaa cacactctgc
     2221 ttaaatggaa tttttacatc ctttagtgat atgattcaaa ctcaaattcc taattcaaag
     2281 aaaattccaa tcgaagaagc aattctacat gttagagaat caatagagta tgaaggtgct
     2341 gaatttgtaa atcccgctta tcctatttta agaaaatcat taaaggcaaa tagcgataag
     2401 ccaccgagta tcgttctgat tctcttagaa agttggactg gtaaatttgt aaagcctatc
     2461 tctaaagatg gattaataga tggaaaagaa gttactccta atttcaataa attaattcaa
     2521 gagggagtct tttttaagcg tttctttgcg acaggcggca gaacctccaa tggattattt
     2581 gcatccctta caggtctacc tgatcgaccg atgataagtg tattacatac tcaagaagcc
     2641 aatgcaagag tttcaggact cggaaaaatt ttaaaacaaa tcgactatca aaacctattt
     2701 atgacgggaa gtagtcttgc attcgaaaat atttctccac atattcaaag atggggattt
     2761 gatagaatta tagatgaaag gaatattgaa tccacaaata aattcaaaaa aggcgtttgg
     2821 ggctatgatg atgcagatgg attgtctcta tttaatgaat acttgcgaga attaaatcca
     2881 gacaaaccgt ttctagggat ttatctaaca atttctactc attatcctta caaagtccct
     2941 gatcctaagt ttgaaatatt tgattcaaaa acgatggact ttgacttcct gaatacatat
     3001 cactatgcgg actatgcaat aggaaagttc attgaagaag caaagaaaga aaaatacttt
     3061 tcaaatacta tttttatatt tgtggcagat catactcatc atagattttt aaatcattat
     3121 gaagatcgaa atatcccatt tctaatttat gcacccaatc gatttaaacc agaaattaga
     3181 aatacaattt cttctcaact agatataatt ccgactatcg ttggacttac tggtaaacca
     3241 ctgttattct ctgcaatggg aaaagattta tttgcttcta atataaaatc ttttgcttat
     3301 ttctgttttg gatacttata tggttggatt gatgaggaac atttttattt tcaaaatata
     3361 ggaatttcta ctaaaacttt gaacttcccg atgactgagc catatataga tagaaataat
     3421 tgtgacatat tacctgaatg taaattaaca gaaagaaaaa caaatgcatt tttaaattta
     3481 ccaattcaac ttctagaaag aaatttaatt tttccttact aaagaatcga cgaatttcca
     3541 aatttcaaga ttcgcactga tctcagccgt aaattttcca aacccaggct tttcttcttc
     3601 aattttccca ggccatccat gacctgctcc ttctaatttt aaaaatcgaa ctggcttacc
     3661 ggatttacaa ttcgaatatg aatgagaaaa aacttttact ccatcatcta actcatttac
     3721 aatttctgca tctggcttga tattgcatcg attccatttg acccaatttc tcacagtctc
     3781 ttcggctcca actgcaattc ctttgttccc gctgataaga ccacctcgat atggaacgat
     3841 tggatcgtta gttccattga taaataaaac cggaactggt tgtttcggtt tttccttact
     3901 taatacatct gacatggtag aaactacact tgcatatcca acaaatgctt ctggatattt
     3961 gaaagccatt cgctgggtca taaaaccacc atttgaatat ccaaatagaa agaatttttt
     4021 attcccaaat tgttttatta aatgctctct aagtgttagc aaaaaggttt catcatttgc
     4081 atccggatct tcttcaggta gttttcttcc atctatccaa gcacgattga gtccagaagg
     4141 gtaaacaata ataaaacctt gctcctcggc taaacgattg aactccgtct tccacataaa
     4201 ctcttctcca gttccacctc ttccatgcag accgattaga attatatttg atttaatttc
     4261 tttaggttta aataaaataa actccctttc cttttcgttt atcaatattt tttcatggag
     4321 taaaatataa tcaattggta tgttaccacg aatcgaatct tttccgcaat ggaggaagat
     4381 aaacaaaaga ataatgagta ttttattcat agtttaatta ataatttatt tccatttttt
     4441 gcaactgatt ttccgttttt tattcctaga agaattctaa aaaatccaaa ctaaaaataa
     4501 tgcgtttttc aatccgatcc tttttgacaa caattgtatt ttgcgagagc gtcccaagct
     4561 tgtgatctaa cgctccttgc agttaaagta aaaaaccgat caaatggcaa ccctgcttct
     4621 tcgagagttt tagctgtcca agtattacaa gtatttgtaa tataatagta accatttgct
     4681 aaaaagaaaa agctattccc ataaattcca actcttgttt ttataatttc accagaatta
     4741 gatcttttaa atgtgtttag aatcgcttga tttaaaatga catgcgcctt tttcgaaatt
     4801 ggaagtttta ttacctctga tccattaaaa tataattttg gatcctttgg aattgccact
     4861 acatgtaaaa cactttccgt tggtaaaaac attgctttta gtgttatctt agtagtaatc
     4921 tcttcggctt cataaaatcc cttatctccc caaccaaatt cataaaatgg attagagcca
     4981 aaatttgagt ttagaaattc aaagtctttt cccaaatttt cctgtgctaa gataatacct
     5041 gtatgccacc cgtggctcac aacatatata aatgtttcct cttcctttgt gtctggtaaa
     5101 taattccaag tcttaaaact agagcaacta atcaaaaaaa gtatgaatcc aattcgtaac
     5161 aatgatcctc cccacttact tatattaaca atctgcgcgc ctgtcgggca atcctgtgaa
     5221 cataaggatt ttcaatcacg aagcgcacga agtgaataaa taataaacct tctcttttct
     5281 tcgtgttctc tgtgctcttc gtggttaatt ctttttcata tctcttaact tgacggcatt
     5341 gcctgtcggg ttcaaagatt caaaactttc ctcagtctaa aatccttact agatagcaag
     5401 ggataacagg cttctgtcac ataatactaa actatttgaa acattatcaa gatttaacat
     5461 agaattattc ttattttgag aattagtttt aaaaaattgt ttatatagaa tctgtctaaa
     5521 aattaataac attatggatt catttttccc aaatcgtttc gattgtattg tagttggtgc
     5581 aggtcatgct ggatcagaag cttcttttat tacagctcgt ggtggtctga gaactttact
     5641 tatcacgatg aacatggaca caatcggtca aatgagttgc aatccagcga ttggcggaat
     5701 tgcaaaaggt cacatggtca gagaagtaga tgcgttaggc ggtctcatgg gacgagtaat
     5761 agatgcaact ggaatccaat ttaaaatgct aaatacttcc aaaggcccct ctgtttgggc
     5821 acctcgtgca caagcagata aaaaagaata ccaactgaaa gtaaaacata ctctagaaaa
     5881 agagaaatta ttatcaatcc gacaagacac agtagaagat gtaattatcg aagacaatag
     5941 agtcgtaggc gttaaaacag gacgaggttt cgaaattttt acaaatcatt tgattttaac
     6001 aactggaacc tttttacaaa gcgttattca cattggaaaa taccaaaagg aatctggtag
     6061 attaggggat ggaacagtct atggactttc tgaatcactc cgtcatgtta attttcgtct
     6121 aggtagatta aaaactggaa ctcctcctag gctgaacaaa aactcaatcg attactccga
     6181 attagcaatt caagatggag atgaggatcc aacaccattt tccttttcaa atgatgcaat
     6241 tacaaggaga cagattccct gtcatataac gtataccaat tctgagaccc atagaattat
     6301 aaatgaaaac ttagaactct ctccaatgta ttctggtcaa atcaaatcaa caggaccaag
     6361 atattgccca tctattgaag ataaaattgt tcgttttgca gagagagagc gtcatcaaat
     6421 tttcctagaa ccagaagggt atgaaaccat ggaggtttat gtaaatggaa catccacgag
     6481 cttacctgaa gaagtgcaat ggaaattgat tcgatctatc aaaggtctag aaaaagcaga
     6541 gatcatgcgc cccggttacg cagttgaata tgattttgtt gatccaactg aattaaatcc
     6601 aactctagaa acaaaaaaaa ttaaaggtct ctatcatgcg ggacaaatca atggaacgac
     6661 aggttacgaa gaagcagcag cccaaggagt cgttgcagca tataacgtta tactctcatt
     6721 tagaaaagag cagccaatga ttttttctag aagtgaaagt tatatcggtg ttctggtaga
     6781 tgatttagtt tacaaaggag tagaagatcc atatcgtatg ttcacaagcc gagcagaaca
     6841 tagattgcta ctcagacaag acaatgcaga taaacgtctg atgaaatatg gtttgcaact
     6901 cggtgttgtg agtaaagacg attaccatcg catggaagaa aaatacaatc gtattgactc
     6961 tctcagacaa aaaatttacg gaatgaattt aaaaccttca gatgcactta ctgaattact
     7021 aaaagctaag aagattgaag ctatcaaatt tggaaccgat atggattctt ttattcgtcg
     7081 tcccgaaatt tctctaagtg attgcaaaga aatcattccc gaactaaata ctatttcaga
     7141 taaagaaatc aaagtattag aaatggaaat aaaatatgaa ggctatataa aacgcgaaca
     7201 gcagatgatt acacaaagga acaaaagtct ttcagtgaaa attccagaag actttgacta
     7261 ttcacagcta acagctttaa aagccgaagc aattgtaaaa ttaacaaaac acaaaccaat
     7321 caatttagaa aaagcatctc aaatttcggg agtagatcct tctgatatag atatacttct
     7381 ctttcatcta acggataaag ccagacaaga agggaaataa ttttcaccta gcaccgattc
     7441 ttaaaaccct ttgtttatcg gtgcccactg actgtgttat tggtgaaaat cttttcaatt
     7501 ttccgtcaga atgagtgtta ataacttatt ctaaaattcg gttcactata attttagaag
     7561 cttcaatttc tttggtagta atacctacca tcatttccgg taaaaatttt gttccacctg
     7621 gccatgtatg acctccccct ctaatttcat acaactcaat tcgatttgca ggacattccc
     7681 atctatggtg aataacgcta gttggatcat tggaatattt tttaggtagc tctgctgatt
     7741 ccttgtttgc gcatcctgct ttatctgccc aaaattttag agttgcatcc gtagagtcaa
     7801 cctctccatg agccttacct aaaaccttaa cagttccacc cttataagga acaagaggat
     7861 cttctgttcc attaaaaaca gtgatcgaaa cggagttttg aggcttacaa agagtatccg
     7921 catttaatgg catattagct gtaattggaa atacttcttt gaatgtcttg gacatttcac
     7981 aagcaagacg aaaagacatc attcctccat tggaaattcc agttagaaaa attttatttt
     8041 catcaattaa agtagattta gagattcttt gaatcaattc ccttataaag ccaacatcat
     8101 ctctatattt gacatctaat tttgtaggat cttccctaaa atcattccac tttttatcat
     8161 taccttgtgg gtaaacaaca atgaatccat tttcttttgc tgctttaaga aaatactctc
     8221 catctattcc gacagagttt tcaggctttc ccccacctcc atgcaaaact agtaagagag
     8281 gttgggcttt ttggaacttt ggatcataaa tcaatgccct acgctcttcc ccattccaat
     8341 caaatttgat ttcatccaat gagttactga actctttaga tgccctaagg cgtctatcgc
     8401 atgatattaa taaaattaga aataataaaa tataaattgt ttttttcata gtaaattagg
     8461 atggaatttt ttttgttaat attttttagt tttaatcttg tcatctaaag aaaacaaatc
     8521 gatataaggt taactactct ccgatgaatt taaaaattct ttcagtagta gtggtctatg
     8581 tttccttata tcgatttatg catcttgata gaattatttc ttagttcttt tactgtcttt
     8641 ggcttcttcc ttatctttat gaaaattctt gaattcatca tccgtaattg atccatcacc
     8701 atctttatca atttcggtaa atcttgtctc atgaaatttt acccattctt cttttgagat
     8761 ttttccatcc ccgtttgtat ccattttttt aaaatgtttt ttcccatgac catgatgacc
     8821 tttctctttt tcatcatctg cgtaaagaga tatagaaact gaaatcacaa ctactaaaaa
     8881 taattttaat gtattattca taattatcct cactgatttc attatcagat tttattctca
     8941 attgaaaagc tttttattat cttacctttt atctaaatta taagattcaa tattcgagtc
     9001 attgagaatg catatagaac ttatttgtct taaataacaa atcttacagc gaagtattgc
     9061 aaagcgagaa aaagggaggt aactccgatc actcaaaact gaaacatcaa ttcaattact
     9121 atatctggaa ctataaccta ctaagtctac ttctctatta atacattaga gaaatgcaat
     9181 atatgaattt cttttatcat tcttatcaat tcatatcttt ttagaaagat catttattaa
     9241 attagaatcg gagattcaaa tcaaatctcg cggataacac tatttaacct ctactactct
     9301 tccctgaaca aagcaagtaa atacactatt tgactttaag aaatttctta tttgtgcagg
     9361 ttctaacact aataatttag gaacgctttc tttgtaaatt tcagtttgag caggaatgtt
     9421 tatgaaacca ttcgcactga attcatctcg aacaaaactt gccttgataa ctatatcctg
     9481 attttccaaa tcctcagaag tatttttcaa tgtaatccaa aatgatgtat gccctgtttt
     9541 aattaatatc ttacttccaa taaagctaat tgatccttta ctttgaacaa ttttttctac
     9601 aacgctatcc gcattcgaag atatcgactg acgttctatc aatctagttc cttgcactcg
     9661 ttcaaaaaca tcactcatat tgtctggatg aggaaagtct accttgctta aaattctacc
     9721 ttcatctaag gctaccattt ctttaagatc aatttctctt ggattaactt gttcagattc
     9781 atcataatag attgttctat catttaaata aattgtctta taacctttag tagttccaac
     9841 tttttgaaaa tgcgttattt gctgcaaagt atttccacca agctttgcag cttttttatg
     9901 aagactcatt gatttataat caaaaaaaga tcctgataca atgaaagtat tataacccaa
     9961 ttctttgatt gcttgaacag cagacaaata tccataatct cctgttccac tgcttttagc
    10021 atctgttaca ataatcattt tcttttttgt aattctattt aaacctaatt cggaacgtat
    10081 ttttaccaat ccttgggtaa ttcgatcaat ccctacatct ccgccctgct taatacgtcc
    10141 taactcttcc tttaatttat catggttcaa agaaggtttt agaatcttta tagaagactc
    10201 actcacgaat actgcaccta acgataaatt tttgcttcca gtaatagaca gtatatatct
    10261 aaagattcct tttacttccc tagaaagaga tccagacatg tcaaaaagaa atataatttc
    10321 ttcctctccg ctaagatcat acgcagaatc cgatttattt tttgggacta gaaagttaag
    10381 agttcttttt gtatgcttct ctatagatga atagaaattt tctttgaata aactttcgtt
    10441 cgaaccaatc acttttcctc tacagttata aacctctgaa agaattgaga tactagaatc
    10501 aaaatttatt tctgttttta caatatagtc agagccaaac tcttcacaga ctccatttaa
    10561 aagagatttg cttggctttc gatccattgt ctcaccggag tctctcacat aattttttac
    10621 tttatccgga tcagaaacct cgattaagaa attttcattt gcataatatg caatcaactc
    10681 agagattcct gaagatacat tgtctttctt ttttaaagca ggaggctcct ctcctatcac
    10741 ataagcgggt agaaaagtaa cttctctagc aaataacgtt gtatttaata aaatagttag
    10801 aagtaataaa attattttaa tcatataagg cttatcttta gaattgtaac ctatccaaga
    10861 attatattca attttgggat ctatataatt ctctccttcc taaaaatccc ttaaagcata
    10921 cagtgaaaag gataaatcaa gaaggagatt gtatggttaa aactatgcaa ggatagttat
    10981 gctagtttta ttctactctc atggtagggc ttgtataaga ataaagtcca tgaaccgatc
    11041 cttgcgcttg tgcagactca gaacgtcttt caaagcgaag agttccatta taaagagctg
    11101 catgcaaatc aggaatcgtt ttatgaccca tatcttgaaa tgattgtctt aaaccttgca
    11161 ctaagtaagg aacaaaatta ataacagagc ctttatcgac taccgcacca cttacccctt
    11221 gtgcaacttt aatcttttgg ctttcgctga aatatctttt gtcaccacct gctttcatag
    11281 cttcaagact tgccattcca cgatattttt taagtcgtat tccattctca taaaaatatt
    11341 caccgggagc ttcattggtt cctgcgaaca tagaacccat catacaagta gatgctccaa
    11401 tagctaaagc atttgcaatg tctccaatgt tactaatacc gccatctgca atcactggaa
    11461 ttccaaattg tgctgcataa cttcctgtca tatacactgc tgttgcctgc gctcttccta
    11521 ctgccattgt ttcttgtgtt atacaaatag aaccaggacc catgcccact cggagtccat
    11581 ctgcacctgc ttcaatcaaa gatctacttt gctcttttgt tacgacgtta cccgccacaa
    11641 catccaaatt tttaaaattt gctttgataa attttatcat ttcaatttga tagctcgaat
    11701 tcccttgagc agagtcaata acgataagat caacacctgc atcatacaaa gctgcgactc
    11761 tatctctaga ttcaggcaaa gtggaaacag ccgccccaac aagtaaacgt ttattgtgat
    11821 ccttagaaga gtctgggaaa tctttattct tttttagatc agaacgactc actagtgcaa
    11881 ctaactttcc atcttggtca ataattggaa gtttacctac tttcttgact ctgagtatat
    11941 tatttgcttc tttcaaacta ataccaacag gtgctgtaac aagagtttta gtcatgacag
    12001 aagatagttt tatttttcga tttggctcaa agtcaatgtc ccgattggtg acaataccaa
    12061 ttaatttacc attgaatgtc ccatcctcag taattggaat tcctgaaaac ccaaactttt
    12121 ctttgatatc atccaaatcc tcaattgtat tttcaggccc gagtataatt ggatctgtaa
    12181 taaaaccatt ttcatatctt ttcacttttc ttacaaaatt tacttgctct gtaattgtgt
    12241 tattataatg tattatgcct aatccacctt gtagagcaag cgcgatagcc atcgcagatt
    12301 cagttactgt gtccatcggc gagctaatga ggggacgttt aattgtgata ttacgactga
    12361 ctttagattc tagatttaca tccgaaggat taaagtcaat atagccaggg agaactaaaa
    12421 aatccctgta tgtgagtccg atttgagccg agaatagttg ctctccggaa agtccgtctt
    12481 gaactggttg atttttaata attgaactgg tcatatccac acttaaaacg ccttgggatg
    12541 aattgcaaga gaaatttaat atgagactgc agagggagcc aaaagaattt ggagattatt
    12601 gacagaaaaa ttcgtgaaac tgtagatatt aaggatgcac ataaacgagt tgtcatcctt
    12661 gccaagcatc taagtaaaac tgaaatgcaa atcaaggata cagaaaccca tctgaaattg
    12721 ctcgagcata atgcagatgg cacaaaaatc atagtagaaa ggctcgatcc aacctacata
    12781 ctcagaaaag ggcaaaatct aattctcaca aaacttctgg gtagatatgt tcaattagac
    12841 tgcacaatcc taggcgaaaa gccagggaat ctttttttac ttcagatcaa taaattttta
    12901 gtatcaagca aagaaagatt aaatgaccga attcatcctc caccagaaca ggtatgggtc
    12961 acaaatatca ggacgagtgg tgtatccatt gaaacaaata tgcacacaat tccaacctac
    13021 atgaaaatca attttacaga ttatgaaaat aaactacgca agaatttcga ttat
//
LOCUS       DAOQOS010000137        13054 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_2454_length_13054_cov_5.761443, whole genome shotgun sequence.
ACCESSION   DAOQOS010000137 DAOQOS010000000
VERSION     DAOQOS010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 13054)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13054)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13054
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2454_length_13054_cov_5.761443"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..858
                     /gene="obgE"
                     /locus_tag="PLF66_21620"
     CDS             <1..858
                     /gene="obgE"
                     /locus_tag="PLF66_21620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002629790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HMZ66702.1"
                     /translation="KVYKASSGVHGGARNSSGANSDDLFLKVPVGTQVIDEDTEEILH
                     DFLKDEERFCVVKGGRGGKGNAFFKSSTHQTPRFAQPGEEGEMRKIRLSLKLLADIGI
                     VGLPNAGKSTLLSKITHAHPKIAGYAFTTLIPNLGVVTRPDESRYTIADIPGIIEGAS
                     QGHGLGLSFLKHIERVKGILYLFDGSSVDIESEFKMLQNELKTYNKALLKKPSIIVLN
                     KIDIWEDEKFTKELVKRYSKLGTVIPISAQNEINLNKLLETIDTKLIKKIDKKKPRAK
                     KVVISSDET"
     gene            848..1681
                     /gene="proB"
                     /locus_tag="PLF66_21625"
     CDS             848..1681
                     /gene="proB"
                     /locus_tag="PLF66_21625"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015678157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HMZ66703.1"
                     /translation="MKRNDFNTYIKNAQRIVIKVGSARVSGDERQMNDFLYSLVGDIR
                     QLFDDKKEIILVSSGAVAQGKKIMSDHSGKTFDKKTIIERQALAAIGQSRLMSLYETF
                     FSRVNIPISQILFGMLDVKETVGAENLRNTFNQLIGWKILPIVNENDSIATEELKLGD
                     NDILSALVTLLMQADLLIILTGVNGFNRNGKDVSFLQEVSDKDLTFAKGPDGPGTGGM
                     NTKLKAGKLLLSANIPTAIINGKEKHCITSFIQENKRGTLIANGKKPKKIKIEEIQKL
                     F"
     gene            complement(1678..2541)
                     /locus_tag="PLF66_21630"
     CDS             complement(1678..2541)
                     /locus_tag="PLF66_21630"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HMZ66704.1"
                     /translation="MDLIVLILSVLVSGGIWLRFLYQHDRVEPEPILVVIKIIFLGGL
                     ISTVAAGTLNSIFSYLSGIPISSMTLSFQKSFILSTFVGFNEEIIKAYITYLLVRNLK
                     DLDEPIDAVIYATSVGLGFSVFENFQYARENGLFNLVLRSLTAMPLHIGLAAIWGYKI
                     AEVKFLHGKNYLDSMRPAVVLAAVLHATYDFFQFYIANPYLPLLISATFSYFMFNTIQ
                     KKLNYLSTQSPFLKAGTCVNCGTMNPIDAIKCKSCGMNLTQNFYKICSQCLSKVPISE
                     SKCPDCNADLD"
     gene            complement(2616..3014)
                     /locus_tag="PLF66_21635"
     CDS             complement(2616..3014)
                     /locus_tag="PLF66_21635"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HMZ66705.1"
                     /translation="MIPSLIIQVDDDEINNFINESVFKSNFKNTTVKSFVNPIKAVQF
                     IKTEFIINPVPAAMFLDINMPEMNGWEILEALNVHLSIIEKYLPIYVVSSSIDSLDKY
                     KSSVNPLIKAFLSKPISNQLIRDIFNVSSK"
     gene            complement(3017..3910)
                     /locus_tag="PLF66_21640"
     CDS             complement(3017..3910)
                     /locus_tag="PLF66_21640"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HMZ66706.1"
                     /translation="MESIWNEIDNNRIWHGEIQLLTKTGKTIWLETSIFPINDSNNKD
                     QSIMAVCSEISERKKREMERERLLNSLAQRNKDLEQFSYIVSHNLRAPLANILGLTNL
                     LKETQIENETTRLIINNLYKASQNLDNVITDLSEILQIRKQIREEKQLVKLSALVDSI
                     YISLESLIAETQATVDVDFTEIDEITSLKGYLHSIFSNMIINSIRYAKAGVPPVIHIK
                     SYKKDFKIILEFSDNGRGIDLKKYADKIFGLYKRFHLDIEGKGVGLYMTKTQVELLGG
                     TIKVESELLKGTTFTIELPLI"
     gene            complement(3959..7735)
                     /locus_tag="PLF66_21645"
     CDS             complement(3959..7735)
                     /locus_tag="PLF66_21645"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HMZ66707.1"
                     /translation="MAKSKKAFYVAGVGSSAGGLEALQDLLSHLPKTLNNVAFIIAQH
                     LSPTHKSMLVQLLSRETKLSVTEAKNGDSIEPNNVYITPPDNEISVTGGKMWLQRPKG
                     GVGPKPSVDVLFQSLADDLGENVIGIILSGTGTDGAIGVRHIKATGGLVIVQEPQTAK
                     YNGMPLASIETGLVDMVLSPDKMGEELLDIAQNESYTRKIKISDIENNANSQQAIFKL
                     LSKRTGTNFSNYKSSTIGRRLEKRLSTLKITSLEDYLTYIEKNPQELDSLFNMILIGV
                     TSFFRDSAAFIEFEKVILKILNSKPEGEPIRIWAPGCATGEEAYSIAILLAKNLKEKV
                     SDFNIQIFATDIDEKAIAIARKGIYSSDSLSEVSEDLVNQFFIKKGNQYELLKSIRSM
                     VLFSKHDLTSNPPFLKLDLLCCRNLLIYFGPELQKQIIPLFHYALKPNGYLFLGKSET
                     VGQYTDLFSTEDAKSKIFQRKRGGKLHAVQFNGFRPESKVYASPVIPRQKGELSLVEI
                     IKETIFNTFEYPYIIVNDALDIQEISGDVGLYISISEGAMNANIFKLIHKDLLIEIRS
                     ILTKAIRDRIQQRTGVLKFAFYGNQHFVRIKVKPLLFSESTKDLFLVIFEKQDMEDFT
                     PVISISDKISDENPRILELEHELQATREHLQTYIEELETSNEELQSLNEEMQSTNEEL
                     QSSNEELETSNEELQSTNEEIQIAYAELKAANDELEEKDKLLMNAKANEKALLDNTLQ
                     SFFLIDKAYTILAFNVTASETIKKIYNKTLKEGDPIFEYISADNLEKFRDNFTEALKG
                     KSSNGEQRILKHDQTMVSYIYNVTPIIKEGGIIDSISLSLLDITNLRNLQIELSSSEN
                     LLASVFETNSIGIAISDKNGHFVNINKVGCEIFGYPKEELIGFHFNKVVSDDFKPNFV
                     EILDKFINTGKDLNAEWTIQRKDKSLVDIYASASLLVQNDGTKYQITSIRDITENKKY
                     KNLLEETQESASVGGWQFDSITQELSWTEETYKVFGVDSSYKLSIENMANLFLDEAGV
                     IFKNSIAKTLTAGEAFDLELKAKSTTNSPLWIRATCKPVSVYNKVIKLFGTFQNITTI
                     KESEIKLKESEDLFRSIYENNKDAILISYFTGEVIKTNASANIIFGYLDSEFSNVTLR
                     SLFPENDPLTTNGFNFLKTNGKFQGETFAIRKSGELFSCEIYSSIFILANGMYRICTI
                     IRDLSEQKKMLSTQERLKNALYLSNIVIIGNRNGIAIEANDNFIQLAEYDKQEF"
     gene            complement(8010..9086)
                     /locus_tag="PLF66_21650"
     CDS             complement(8010..9086)
                     /locus_tag="PLF66_21650"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004433375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HMZ66708.1"
                     /translation="MIKVVLLIGGKSSEHEISLKSSYFIFNTLDRSKYEVTPILIDKK
                     GNWIIPESKDAKYPNIQSIQKTFPIESLADEFKKQFIAENQILHSEGISISDIDCDIA
                     FLGLHGGDGENGVIQAFLQMYSIPFTGSGVLASALAMDKERSNYLFLSAGLTVANFVN
                     VNKKEFSLKGEGFLNNFHLTFPVFIKPTHGGSSVGVGKANNILEVATKLRELFNTEES
                     VLIQENISGIEVSCGVLEKKTGRDFEVIALHPTEIVPKSEFFDYKAKYQIGHSEEITP
                     ARISDELTASIRESALLAHKILGCKGYSRTDFIIQDGKPYILETNTLPGMTETSLIPQ
                     QAKYAGFQMNEVFDWLIENGLSLH"
     gene            complement(9088..9960)
                     /locus_tag="PLF66_21655"
     CDS             complement(9088..9960)
                     /locus_tag="PLF66_21655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HMZ66709.1"
                     /translation="MTDFFGTIVNIDDREFNYLKDIIYEKTGISLAPHKKIMLQSRLN
                     IRLRHNQLSSFVEYVYKLKNDRNFMNSEIPEIINRITTNKTDFFRENHHFEFLKTTCF
                     PLFEEKAKTSGKRKIRIWSSASSTGEEPYTLAMTVNDYFIGKPGWDIKIYASDIDTNV
                     LDTAQRGVYREDRMAPVKPELRTKYFTSKITEKEKEYIAKPVLKDLLIFKKINLLEDP
                     YPIQEKIDIIFCRNVIIYFDKQTQQKIFSQMEKILTDDGFLIIGHSETLFGISDNFKF
                     LGHTIYQKKSPNGK"
     gene            complement(9975..10046)
                     /locus_tag="PLF66_21660"
     tRNA            complement(9975..10046)
                     /locus_tag="PLF66_21660"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10011..10013),aa:Arg,seq:tcg)
     gene            complement(10104..11525)
                     /locus_tag="PLF66_21665"
     CDS             complement(10104..11525)
                     /locus_tag="PLF66_21665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010413181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HMZ66710.1"
                     /translation="MQNKKVDTSIESSESIWENLKDAMKGTEADYTQINLKKAIFLLA
                     VPMILELIMESTFAVADIYFVGKLGPSAIATVGISETFLFLLYSIAMGIATGVTAIVA
                     RRVGEKNQKEATNASVQSIWLGIIASCPFFILGYFYSQDLLRLMGADQWTIDHGYLYT
                     KWILSGNIVIMLLFILNAIFRGVGDAAIAMRVLWIANTINIILDPILIFGYGPIPPLG
                     IQGAAIATNIGRGTGVLVQIWTLFNAGKHIKVFVADLYWDLKTILHILGTSIGGIGQM
                     IVASTSWIFLMSILAKVGSEAVAGSTITIRIMMFTLMPAWGMSNAAATLVGQNLGAGN
                     AKRAESAVWKIGFYNMIFLLFVSILYYTNSKELIRIFSDNPVVIQIGSEWLQILSYSY
                     LIYGWWMVSAQAFNGAGDTKTPTYINLIFFWCIQIPLCYYLALFLEWKYTGVFWGVFI
                     SETSVGIFTLWLFSRGSWKKTKV"
     gene            complement(11828..12424)
                     /gene="metW"
                     /locus_tag="PLF66_21670"
     CDS             complement(11828..12424)
                     /gene="metW"
                     /locus_tag="PLF66_21670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016544399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HMZ66711.1"
                     /translation="MKSKQLNLRLDFQYILDLIPTGHRVLDLGCGDGDLLYLLKRKGV
                     RGQGIEKNEDCIYKCIERGVIVHHGDLDEGLKHHIKKSFDYVILNQTIQETHNPGNII
                     KDSLRIGKKVIVVFPNFAHWQNRLQVLFRGITPVTDNLPYRWYNTPNLHFLSVTDFQE
                     FCIDHRIHIEDSAFFSDRKKINFRPNFFSKLALFVLTE"
     gene            complement(12414..>13054)
                     /locus_tag="PLF66_21675"
     CDS             complement(12414..>13054)
                     /locus_tag="PLF66_21675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004468648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HMZ66712.1"
                     /translation="SVAYPSMVKNCIILASASEHSAQQIAFNEVGRQAILSDPNWNNG
                     EYYDNPPKKGLALARMVGHVTYLSDELMREKFGRKPPVGNIKNTDFAVGSYLIYQGES
                     FVDRFDANSYIYVTKALDHFSLGRGEELTRNLSVARANFLIIAFSSDWLYPPYQSREI
                     VKSLEVNAIPVTYCEVNFSKGHDSFLVPNPEQHHLIHHFLEFAQNFSLFYEI"
BASE COUNT         4250 a         2156 c         2137 g         4511 t
ORIGIN      
        1 aaagtgtata aagcaagttc cggtgtacat gggggagctc gaaattctag tggtgcaaat
       61 agtgatgatc tttttttaaa agttccagtt ggaactcaag tgatcgatga agacacagaa
      121 gagattcttc atgattttct aaaagatgaa gagcgttttt gtgtagtgaa agggggcaga
      181 gggggaaaag gaaatgcatt tttcaaatcc tctacacatc aaactcctag atttgcccaa
      241 ccaggagagg agggcgaaat gcggaaaatt cgtcttagcc tcaagttact agctgatatc
      301 ggaatagtag gtcttccaaa tgcaggtaag tctactcttt taagtaaaat tacacatgct
      361 catcccaaaa ttgccggata tgcttttaca acattaattc cgaatttagg ggttgttaca
      421 agacccgacg aaagtcgtta tacgatagcc gatattcctg gaattataga aggggcaagt
      481 caaggacatg gtcttggatt gtctttttta aaacacattg aaagagtaaa aggaatactc
      541 tatttatttg atggaagcag tgtagatatt gaatcagagt ttaaaatgct acaaaatgag
      601 ttaaagactt ataataaggc gcttcttaaa aaaccaagca taatcgttct aaataaaatt
      661 gatatttggg aagatgaaaa atttacaaaa gaattagtaa aaagatactc taagctagga
      721 acagtgattc caatttctgc acagaatgaa atcaatttaa ataaactttt agaaacgata
      781 gatacaaaac ttatcaaaaa aattgataag aaaaaaccaa gagccaaaaa agtggtaata
      841 agctcagatg aaacgtaatg actttaatac ttatataaaa aatgcccaaa ggatagtaat
      901 caaagttgga tctgctcgtg tatcgggtga tgaacgtcaa atgaatgatt ttctatacag
      961 tctagtaggc gatatacgac aactctttga tgataaaaag gaaattatat tagtttctag
     1021 tggtgcagtt gcacagggaa aaaagatcat gtccgatcat tccggaaaga catttgataa
     1081 aaaaacaatc attgagcgtc aagcacttgc tgcaattgga caaagtagac ttatgagtct
     1141 ttatgaaaca tttttttcta gggtaaatat tcctatctca caaatcctct ttggaatgct
     1201 agatgtaaaa gagacagttg gagccgaaaa tttaagaaat acttttaatc aattaatcgg
     1261 ttggaagatt ctcccaattg taaatgaaaa tgattctata gccacagaag agttgaaatt
     1321 aggggataat gatatattat ctgctttagt tactctttta atgcaggcag atttactcat
     1381 tattttgaca ggcgtgaatg gttttaatag aaatggaaag gatgtatcct ttttacagga
     1441 agtgtccgat aaagatttaa catttgcaaa aggtcctgat ggtcctggaa caggtggaat
     1501 gaataccaag ttaaaagcag gcaaattatt attatccgcc aatataccta cagcgattat
     1561 caatggaaaa gagaaacatt gtattacaag tttcatccag gaaaacaaaa gaggaacact
     1621 aatcgcaaat ggaaaaaagc ctaaaaaaat taaaatagaa gaaattcaaa aactattcta
     1681 atctaaatca gcattacagt ctggacattt actttcagaa ataggaactt tcgatagaca
     1741 ttgactacaa attttataaa aattttgagt caagttcata ccacaagatt tacatttaat
     1801 tgcatctata ggattcattg ttccacaatt cacacaagtc cctgctttta aaaaaggact
     1861 ttgggtagat aaataattca attttttctg aattgtgtta aacataaaat aggaaaaagt
     1921 agcgctaatc aatagaggta aataaggatt tgctatataa aattgaaaga aatcataagt
     1981 agcgtgaaga actgcagcta gaactacagc gggtcgcata gaatctagat agttttttcc
     2041 atgtaaaaat ttaacttctg caattttata tccccatata gcagcgagac ctatatgtaa
     2101 aggcatagcg gttaacgatc ttagaactaa attaaataat ccattttccc tagcgtattg
     2161 aaagttttca aatacagaaa aacctagacc aacggaggtt gcataaatca ctgcatcaat
     2221 tggttcatct agatccttta aatttcttac caaaaggtag gttatatagg cttttataat
     2281 ctcttcgtta aatcccacaa acgtagatag aataaaactt ttttgaaaac ttagagtcat
     2341 tgaagaaatt ggaattccgg ataagtagga aaaaatacta tttagtgttc cagcggcaac
     2401 tgtgctaatg agtccaccta aaaaaataat ttttataact actaaaattg gctcaggctc
     2461 tacacgatca tgttgatata aaaaacgcag ccaaattcca ccgctgacta atacagagag
     2521 tattaataca attaaatcca ttccaataca gtgttctttt aactaatcct gtcaagcttc
     2581 aaatcaaaag aattaggaaa ggattctatt ttatcttatt tggaactaac attgaaaata
     2641 tctcgaatta attgattgga gataggtttt gatagaaaag cctttatcaa tggattaacg
     2701 gaagatttat atttatctaa actgtcgata gaggaagaga caacataaat tggtaaatac
     2761 ttctcaataa tacttaaatg gacattcaac gcttccaaga tttcccatcc attcatttca
     2821 ggcatattga tatctaaaaa catggcagca ggaacgggat ttataataaa ttcagtctta
     2881 ataaactgaa cagctttgat gggattaaca aaagatttta ccgttgtgtt tttaaaattt
     2941 gatttaaata cggattcatt tataaaatta tttatttcat catcatcaac ttgaataatt
     3001 aaagagggta tcatatttaa atcaaaggta attctatagt aaatgtagtg ccttttagta
     3061 attcgctttc aacttttata gttccaccaa gcagttcaac ctgtgttttg gtcatataaa
     3121 gaccaacacc ttttccttca atatccaaat gaaatctttt atacaatcca aatattttat
     3181 ctgcatattt tttaagatca atcccacgcc cgttatccga gaattctagt attattttaa
     3241 aatctttttt ataagatttt atatgtatga ctggaggaac gcctgctttc gcatatcgta
     3301 tactatttat aatcatattt gaaaagatac tatgcaaata tccttttagt gatgtgatct
     3361 catctatttc agtaaaatct acatcaacgg ttgcttgtgt ttctgcaatt aggctttcta
     3421 gactaatata gattgaatca acaagcgcag aaagttttac taattgcttt tcttctctaa
     3481 tttgttttct gatttgtaaa atctcactca aatctgtaat tacattatct aaattttgag
     3541 aagctttata tagattattg ataattagtc ttgttgtttc attttcgatc tgtgtttctt
     3601 taagtaaatt agttaagcct aaaatatttg caagaggtgc acgtaagtta tgagaaacta
     3661 tatatgaaaa ttgttccaag tctttgtttc tttgggctaa actatttaaa agtctctctc
     3721 tttccatttc tctttttttg cgctctgaaa tttcagaaca aacagccatg atagattggt
     3781 ctttattatt cgaatcattg attggaaaaa tgctagtctc aagccagata gtttttccgg
     3841 tcttcgttag taattgaatt tctccatgcc aaattctatt attatcaatt tcattccaaa
     3901 tagactctat ttgtttagga tctaggtttt ttgaatctaa aaatttaaat ttgagatttt
     3961 agaactcttg tttatcatat tcagctaatt ggataaaatt atcatttgcc tctatggcaa
     4021 ttccgtttct atttccaatg attacaatat tacttaagta tagggcattt ttaagacgct
     4081 cttgagtgct tagcatcttc ttttgctcgg ataaatctcg tataattgtg cagattctat
     4141 acattccatt tgctaatatg aatatactgc tataaatttc acacgagaat aactctccag
     4201 attttcgaat tgcaaatgtc tctccttgaa atttaccatt tgttttcaaa aaattaaaac
     4261 cgttagtagt taaaggatca ttttctggaa ataaacttct taaagtaaca ttggaaaact
     4321 cggaatctag atatccaaat attatatttg ctgaagcatt tgtctttatg acttctccag
     4381 taaaatagct aattagaatc gcatctttat tattctcata aatagatcta aataagtctt
     4441 cactttcttt tagttttatt tcggactctt ttatggtagt gatattttgg aatgtcccaa
     4501 atagtttaat gactttatta tatacagata ccggtttgca tgttgctcga atccagagtg
     4561 gtgagtttgt agtagattta gcttttaatt ctaaatcaaa tgcttcacct gctgttaagg
     4621 tttttgcaat ggagttttta aagataacac ctgcttcatc taaaaataaa ttagccatat
     4681 tttctatgga aagcttatag gatgaatcta ctccaaatac tttataggtt tcttctgtcc
     4741 atgatagttc ttgtgtaatc gaatcgaatt gccatccacc tacactagct gactcttgcg
     4801 tttcttctaa aagatttttg tattttttat tttcagtaat gtctcgaata gacgttattt
     4861 gatacttcgt tccatcattt tgaactaaaa gagaagcaga tgcataaata tctactaaag
     4921 atttatcctt tctttggatc gtccattctg catttaaatc ttttcctgta tttataaatt
     4981 tatctaaaat ttctacaaaa ttaggtttaa agtcatcaga aacaacttta ttaaagtgga
     5041 acccaattag ttcttctttg ggatatccaa atatttcaca acccacttta tttatattaa
     5101 cgaaatgtcc atttttatct gagattgcaa taccaatcga gtttgtttca aatacagatg
     5161 ctagtagatt ttcagaggat gacaattcga tctgtaaatt tctgagattt gttatatcta
     5221 aaagtgataa tgagatacta tctatgatac caccttcttt aatgatagga gtaacgttat
     5281 atatatagct aaccattgtt tggtcatgct ttaggattct ttgttcgccg ttacttgatt
     5341 ttcctttcaa agcttctgta aaattatctc taaatttttc tagattatca gcggagatat
     5401 attcaaaaat aggatcccct tcttttaatg ttttattata aattttcttt attgtttcac
     5461 ttgctgtaac attgaatgct aaaattgtat atgctttatc aattaaaaag aaactttgca
     5521 atgtattatc gagtaatgcc ttttcatttg cttttgcatt cattaaaagt ttgtcttttt
     5581 cttctagctc atcgttagcc gctttcaatt ctgcatatgc aatttgaatt tcctcatttg
     5641 tggattgtaa ttcttcattg gatgtttcta attcttcatt tgaagattgc aactcctcat
     5701 ttgtagattg catttcttca ttgagagatt gcaattcttc atttgatgtt tcgagttctt
     5761 caatataggt ttgtaaatgt tctctagtag cctgcagttc atgttctagt tctagaatac
     5821 gaggattttc atcgcttatt ttatctgaaa ttgatatgac aggagtaaaa tcttccatgt
     5881 cttgtttctc aaagattact agaaacaaat ctttagtgct ttctgagaat aacagcggtt
     5941 ttactttgat tctaacaaag tgttgatttc cataaaaagc aaatttaagc acacctgttc
     6001 tttgctgaat tctatcacgg atcgcttttg taagaatcga tctaatctcg attaataaat
     6061 ctttatgaat taatttgaaa atattagcat tcattgctcc ttctgaaatg ctaatataga
     6121 gaccaacgtc tcctgaaatc tcctgaatat caagagcgtc atttactatt atgtatggat
     6181 attcaaaggt attaaagatt gtttctttaa taatttcaac gagtgacaat tcgccttttt
     6241 gtcttggaat tacagggctt gcataaactt ttgactcagg tcgaaatcca ttgaattgga
     6301 ctgcgtgtag ttttcctcca cgctttcttt ggaaaatttt agattttgca tcttctgtag
     6361 agaataaatc tgtgtattgt ccgactgttt ctgatttacc taaaaacaaa tatccattag
     6421 gttttaatgc ataatgaaac aatggaatga tttgtttttg cagttcggga ccaaaataaa
     6481 taagtaaatt tctacaacaa agtagatcta gttttaaaaa tggtggattg cttgttaaat
     6541 catgtttaga aaagagaacc atcgaacgaa ttgattttaa aagttcatat tggtttcctt
     6601 ttttaataaa gaactgattt actaaatcct ctgaaacctc gcttagagaa tcggaagaat
     6661 aaattccttt tcttgcaatg gcaattgctt tttcgtctat atctgttgca aagatttgaa
     6721 tgttgaaatc agagactttc tctttcaaat tttttgctag aagaattgca atagagtagg
     6781 cttcttcgcc tgtcgcacaa cctggagccc agattcgaat gggttcgcct tctggcttag
     6841 aatttaagat ttttaaaatt actttttcga actcaataaa tgcagcggag tctctaaaga
     6901 aagaggtaac tccaatcaaa atcatattga agagagaatc taattcttgt ggattctttt
     6961 ctatataagt gaggtagtct tcgagagagg taatttttaa agtagagagt cttttctcta
     7021 gtcttctccc tatcgtagaa gatttataat ttgagaaatt ggttcctgtt cttttcgata
     7081 atagtttaaa aattgcttgt tggctatttg cgttattctc tatatcagaa attttaattt
     7141 tcctagtata ggattcattt tgagctatat cgagtaattc ttctcccatt ttatccggag
     7201 aaagaaccat atcaactaaa cctgtttcta tagatgcgag aggcatacca ttgtatttcg
     7261 cagtttgtgg ttcttgaaca atcaccagac caccggtagc ttttatatga cgaactccaa
     7321 ttgccccatc tgttcccgtt cccgagagaa taataccaat tacattttcc cctaagtcat
     7381 cagctaggga ctgaaataaa acatcaacgg atggcttagg accaactcca ccctttggtc
     7441 gttgcagcca catttttcct cctgttacac tgatttcatt gtctggcgga gttatataga
     7501 cattgtttgg ctcaatggag tctccatttt ttgcttctgt gactgaaagt ttggtttctc
     7561 tgctcagtaa ttgaacaagc atacttttgt gagtggggct taaatgttgt gcaatgataa
     7621 atgcaacatt gtttagagtc tttggaaggt gggatagtaa atcctgtaat gcttctaatc
     7681 ctccggctga ggaaccgaca cccgctacat aaaatgcttt tttacttttt gccaattaag
     7741 ataacctttt tatccaagaa tattcccgat taatttacat ataatccaat agtaattttt
     7801 gaaaagtatt tttcttatct aggagttttt tccattttaa tagtgtattt taatctctag
     7861 gctgagtttg agttttgtta tttaaaattg aatttggtta tatatataaa gggaagatca
     7921 caaggtaaac ttgactttat ttcaatcttt tttgcgatta acgttcgaag gcatagtgtt
     7981 tgaattttgc aatgataaaa atcatagaac taatggaggc taagtccatt ctcaataagc
     8041 caatcaaaca cttcattcat ttgaaagcct gcatatttag cttgctgcgg aattaaacta
     8101 gtttcagtca ttccaggaag agtgttagtc tctaaaatat aaggctttcc atcttgtatt
     8161 ataaaatcag ttctagaata gcctttacaa cctaaaattt tatgagccag taaagcagat
     8221 tcgcggatac tcgctgttaa ctcgtctgat attctagcag gagtaatttc ttctgagtgt
     8281 cctatttgat actttgcttt gtaatcaaag aattcacttt ttggaacaat ttctgttgga
     8341 tggagagcaa tgacttcaaa atctctacct gttttttttt cgagaacgcc gcacgagact
     8401 tctattcctg atatgttttc ttggattagc acagattcct cagtattaaa tagttctcta
     8461 agttttgttg cgacttctag tatattgttt gcttttccta cacctacact tgaacctcca
     8521 tgggttggct ttataaaaac gggaaaggtc aaatgaaaat tgtttaagaa gccttctcct
     8581 tttaaggaaa attctttttt gtttacattc acgaaattag caaccgttag accagccgat
     8641 aaaaataaat agtttgatct ttctttgtcc atagcgagag cggaagcaag aacacctgat
     8701 cctgtaaatg ggatggaata catttgtaaa aaagcttgga ttactccatt ctcaccatct
     8761 cccccatgta aacctagaaa agctatatca cagtcgatat ccgaaataga aattccttcg
     8821 gaatgtagga tttgattctc tgctataaat tgttttttaa attcatcagc taaactttcg
     8881 ataggaaatg ttttttggat actttgaata ttgggatatt tcgcatcctt actttctggg
     8941 ataatccaat ttcctttttt atcaatcaga attggagtta cttcatactt tgatctatcc
     9001 aatgtattaa aaataaaata agaacttttg agtgaaattt cgtgctcgga agattttcct
     9061 ccgattaaaa gaacaacttt tatcatgtta cttcccattt ggagattttt tttggtaaat
     9121 ggtatgccct aaaaatttaa agttatccga aatcccgaat aacgtttcag agtgaccaat
     9181 aatcaagaaa ccgtcatctg ttaagatttt ttccatttgg ctaaaaatct tttgttgggt
     9241 ctgtttatca aaataaataa ttacgtttct acaaaagatg atatcaattt tttcttggat
     9301 aggataggga tcctctagaa gattgatttt tttgaatatt aataaatctt ttaatactgg
     9361 ttttgcaata tattcttttt ctttttcagt aattttagat gtaaaatatt tcgtccgaag
     9421 ctctggtttt acaggagcca ttcggtcttc tctgtaaacg cctctttgag cagtgtctag
     9481 aacgttagtg tcaatgtcag atgcatagat ttttatatcc caacctggct ttccaataaa
     9541 gtaatcattg actgtcatag ctagagtgta aggctcttcg ccggttgagg aagcagaaga
     9601 ccagattcga atttttcttt taccactagt ctttgccttt tcttcaaata gaggaaagca
     9661 ggtagtcttt agaaattcaa aatggtgatt ctctctaaaa aaatcagttt tatttgtagt
     9721 gattctattt atgatttctg gaatttcaga attcataaag ttcctatcgt ttttcaactt
     9781 gtagacgtat tctacgaaac tactgagttg gttatgacga agtcttatat tgagtctaga
     9841 ttgcagcatt atctttttat gaggcgctag agagattcca gtcttttcat aaatgatatc
     9901 tttcaggtag ttaaattctc tatcatcaat atttacgatg gtgccaaaaa aatcagtcaa
     9961 aagtctacct ctattgccca gagagggaat cgaaccccca ctcacagttc cgaagactgt
    10021 tgttctatcc gttgaactat ctgggcagtg aatctcaata aaattgaaag ttcaatttaa
    10081 ggcaagaatt taatagttaa aaattaaacc tttgtttttt tccaactccc tcgggagaaa
    10141 agccaaagag taaaaattcc gacggatgtt tcagaaataa aaactcccca aaaaacgcct
    10201 gtgtatttcc attccaaaaa tagagccaag taatagcata aaggaatttg aatgcaccag
    10261 aaaaatatta aattaatata ggttggagtt tttgtatccc cagctccatt aaaagcttgg
    10321 gcagatacca tccaccaccc ataaattaaa taagaataag atagtatttg aagccattca
    10381 gatcctattt ggattacaac tggattgtca gaaaaaattc taatgagttc cttgctattt
    10441 gtgtaataaa gaattgatac aaagagtaaa aaaatcatat tataaaaacc aattttccat
    10501 acagctgatt ctgctctttt ggcatttcct gcacctaagt tttgaccaac tagggttgca
    10561 gctgcatttg acattcccca agcaggcatt aaagtgaaca tcataattcg aatggttata
    10621 gttgaccctg ctactgcttc gcttccgact ttagctagga tactcattaa aaaaatccaa
    10681 gaggtagaag caactatcat ttgtcctatt ccgcctatcg atgtcccaag aatatgtagg
    10741 atcgttttta agtcccagta aagatctgcc acaaaaactt taatatgttt acctgcattg
    10801 aataatgtcc agatttgaac aagaactcca gttcctcgtc caatattggt agcgatagcc
    10861 gcaccttgaa tacctagggg tggaataggt ccatatccaa agattaaaat tggatctaaa
    10921 ataatattaa ttgtattcgc aatccataat actctcatag caattgccgc atctccaacg
    10981 cctctaaaaa ttgcattcaa gatgaataaa agcatgatta caatatttcc agataaaatc
    11041 catttagtat aaagatatcc atgatcaatc gtccattgat ctgctcccat gagtcggagt
    11101 aagtcttgag aataaaagta tcccagaata aaaaaggggc aactcgctat tatccctaac
    11161 caaatagatt gcacagaagc attcgttgct tctttttgat ttttttctcc aactcttctt
    11221 gcaacaatag ctgtaacccc agtcgcgatt cccatcgcaa tagaataaag taaaaataaa
    11281 aatgtctcgc taatgcctac agttgcgatg gctgaaggac cgagtttacc aacaaagtaa
    11341 atatctgcga ctgcaaatgt tgattccatt attagttcta gaatcattgg aactgctaaa
    11401 agaaagatag ctttctttag attgatttga gtataatcag cttctgttcc tttcatcgca
    11461 tcttttaagt tttcccaaat tgattctgat gactcgatgc ttgtgtctac ttttttgttt
    11521 tgcatgttat cccttctaga tttttaagga tttagattta aaaaacataa tttgaaacga
    11581 agaagaaatt gattcttacc taataatttt ggaatgcccg agtgtatttt aattaggttc
    11641 aatttttcag tgaatgaaaa aagaaaattg agataagttc ttagtcgatt tcaaatatag
    11701 tgtattttct aatatatttt ttagaggcat ttaagttgca atgttttaaa aataaaaaac
    11761 accaatgaat gaataactgt tagcgaatag aaaaaataca aatgaaatta caatgaatat
    11821 attagaatta ttccgttaac acaaacaatg caagcttgga aaaaaaattc ggtctaaagt
    11881 ttattttctt tcgatcacta aagaaggcag aatcttcaat atgaatccga tgatctatac
    11941 aaaattcttg gaaatcagta acagatagaa aatgaagatt gggagtgtta taccacctgt
    12001 aaggaaggtt gtcggttact ggtgttatac ctctaaataa tacctgaagt ctattttgcc
    12061 aatgagcaaa attaggaaat acaacaatga cttttttgcc tatgcgaaga gagtccttga
    12121 tgatatttcc aggattatga gtttcttgaa ttgtttggtt taaaattacg taatcaaaac
    12181 ttttcttgat atggtgtttt aatccttcat ctaaatctcc atgatgcacg attacaccgc
    12241 gctctatgca tttatagata cagtcttcat ttttctcaat gccctgtccc ctaacgcctt
    12301 ttcttttcag taaatagagt aggtcaccat ccccacatcc taaatcgagc acgcgatgac
    12361 ccgtcgggat aaggtcgagt atatattgaa agtctagtcg taggtttaat tgtttagatt
    12421 tcatagaata aagaaaaatt ttgtgcaaat tctaaaaagt gatgaatgag atgatgctgc
    12481 tctggattcg gaaccaaaaa agaatcatgt ccttttgaaa aattgacttc acagtaagta
    12541 actggaattg catttacttc gagtgattta acaatttctc tactttgata gggaggatag
    12601 agccaatcag aactaaaggc aatgattaaa aagttagctc ttgcaacaga aaggtttcga
    12661 gtcaattctt ctcctcttcc taaactaaaa tggtctaacg cttttgttac atagatatag
    12721 gagtttgcat caaagcgatc tacgaaggac tccccttgat aaattaaata gctacccact
    12781 gcaaagtcag tattttttat atttccaaca ggaggcttac gaccaaattt ttctctcatt
    12841 aactcatctg aaagatacgt tacgtgtcct accatacgag caagagccaa acctttctta
    12901 ggaggattgt cataatattc tccattattc caattcggat cagaaagaat cgcctgacgt
    12961 cctacttcat tgaatgcaat ttgttgagca ctgtgttcag aagcagatgc aagtataatg
    13021 caatttttta ccatagaagg ataggcaaca ctcc
//
LOCUS       DAOQOS010000138        13003 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_2473_length_13003_cov_5.383998, whole genome shotgun sequence.
ACCESSION   DAOQOS010000138 DAOQOS010000000
VERSION     DAOQOS010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 13003)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13003)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13003
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2473_length_13003_cov_5.383998"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(73..273)
                     /locus_tag="PLF66_21680"
     CDS             complement(73..273)
                     /locus_tag="PLF66_21680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66713.1"
                     /translation="MDLFFKQQNLKRKEEGREEGFKIGFKKRNERRKKETFTNTVKLL
                     SAKGFPKKEIDKLLNRMPSDLK"
     gene            509..1531
                     /locus_tag="PLF66_21685"
     CDS             509..1531
                     /locus_tag="PLF66_21685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66714.1"
                     /translation="MNKEVTDPKVIHQLIDSLYRITPTYMEVDGEDIPVKVLDNRPQS
                     VTIRTPKVNSHTRERVLTMRSKGNIFISTFLEVGMDDLGNCILQPLKLLIKDALNQED
                     TNQFIVSNVINQNDIFKSLSDDRINQLIKKAPKVNFMFDFFEVYINERMNSRMRLMNA
                     HEKPIFIPNISQPELVPQNFVPFNEYIALTKSSNVLDKYKSEICVPIKYKNFIMIGYV
                     HAMHSTRLDSNSFNTFKLVASSIIKDIHSIGVFEESRESCKVIEANPGSIRFLHPPTR
                     LASKIFSMGEVVIFDLIAKSGHRKFVRGVIKGIKPTQKDYAIGCDLSSNTPEEIAALT
                     EFLGRH"
     gene            1595..2599
                     /locus_tag="PLF66_21690"
     CDS             1595..2599
                     /locus_tag="PLF66_21690"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015676444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HMZ66715.1"
                     /translation="MKIGLIGSGSFGTALGSLLADKGYDVLMWTRSPEQAEGINLHHK
                     NKKHLSDLVLPENLKATTNLEEVVRDKDLLLSAPPSHVLTEIIHKIKDYISPNCPIVS
                     ATKGIENESLRMVSEIFESELPGRLHKNLSYLSGPSFAREIISRVPTVVSIASKNEGT
                     AKTVQEIFSFTYFRTYWTPDVVGVELGGSLKNVIAIAAGVVDGLGQGQNTRAAVITRG
                     LNEITRLGVKKGADPLTFLGPSGIGDLVLTCCGDLSRNRTVGMKLGQGKTLSEILAEM
                     NEVAEGVKTTKSAYDLSKKLGVSMPITEEVYKMLYEEKSPREVMRDLMGRDLKREGIH
                     "
     gene            2601..3452
                     /locus_tag="PLF66_21695"
     CDS             2601..3452
                     /locus_tag="PLF66_21695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66716.1"
                     /translation="MIYIVTALAQEAIPFIQHFKLKKDLSHTKFDIYKNELITLVVSG
                     TGKLKSSIATTYLLVKEPPKKSDKILNFGICGSGSEKHKSGQIFLINKIEDVTTAKTY
                     YPEILFSHLIPEAGIVTHEKPVVKTKSTPSTAGLVDMEVSGFFEAANTFVQSHNIIIM
                     KVVSDHLSGEKFTGAFIQSIVEKNIPHVITVLNQSRKKQSTAVSDSEILDSNEYRIFE
                     DLSEDLGLSTTQRFQVLDLIKGYKIRGEGDLNFLKDYSKSKAIHSKQDTKKALEELKQ
                     KLDTSGE"
     assembly_gap    3579..3678
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3734..5365
                     /locus_tag="PLF66_21700"
     CDS             3734..5365
                     /locus_tag="PLF66_21700"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HMZ66717.1"
                     /translation="MSEAEEKIDLFSAEEAVIAQGNSILKNKEFTDNPLLEEYKSLLE
                     HYRKLLNQDKKLIKISDGLQNKVKKAKNEIENLNEISRQISMSLDFDIVFGSIFEYIK
                     GGYGFTGCCLTLVSQDKSKCKNEKFISTSEFDSLVETYTGRNYQLKFGNGPITDCILK
                     NEVGIYHDQNIFDMEDSSHAVKNAFLMPISIAGEVIGAFSLLDNSGINLKSDEKESLK
                     KFANHISVVIKNSKIYEDSEKARIKQLEEFNMNLTLINKSLQKFVPKDFVQFLDKENI
                     TEVRLGDSVQQNMSILFSDIRSFTTISESMSPKDNFGFLNSYLKVTGPVIRKNKGFID
                     KYIGDAIMALFPDSASDAVNAGIEMLDEVHKLNVKRKEWGRLPIQIGIGIHTGAPMLG
                     IIGEEKRFEFTVISDAVNLASRIEGLTKAYSASILISEDTLAALQNPDKYIYRMVDKV
                     KVKGKNNPVIIYEILNGNSERIINLKLSTKKKFEEGVELYLEEKFDDASILFKEILKI
                     DPKDTAVSLYLERAKYYSVHGVPLNWEGAWKLDSK"
     gene            5494..6516
                     /locus_tag="PLF66_21705"
     CDS             5494..6516
                     /locus_tag="PLF66_21705"
                     /inference="COORDINATES: protein motif:HMM:NF013246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family ion channel"
                     /protein_id="HMZ66718.1"
                     /translation="MHIGKAYTFKEVINWTRRDILQLGIISTIPVILYIQFQWKWLQL
                     PWLPIALIGTAVAFVVGFKNNAAYDRLWEARRIWGSITNLSRSWGVMVRDYVTNQHSK
                     NPLSDTDLSKIHLELYNRHFAWLTAMRFQLRENKPWEAINLKHNLEYRNTYFTVQEHT
                     QDVKDVLKKYLTEAEFNKVIGKTNKASYILALQSEHLKRLFELGLIDNFRHMELEKIL
                     IECYNQQGGSERIKNFPYPRHFASLNAWFIKIFIFLMPFGMIEEFEKLGPEFVWLTIP
                     FASLAGWIFITMERIGESTENPFEGSANDVPISTISHNIEIDMLELLDLQHNLKSISA
                     QNNILT"
     gene            6516..7166
                     /locus_tag="PLF66_21710"
     CDS             6516..7166
                     /locus_tag="PLF66_21710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003776553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HMZ66719.1"
                     /translation="MKFETIFKNNEKWVEAKLKLDPQYFEKLSKGQAPEFLYIGCSDS
                     RVTAEDLMGVNPGEVFIHRNIANQVVPTDNNINSVIQYAVEHLKVKHIIICGHYECGG
                     VKAALHPSDMGQLNSWLQTLRDVRRIHRAELEQIKDEQELFDRLVELNVREQCMNIIK
                     IDHVQRSWYKTGFPKIHGWIFDVRTGKLKDSGLNFEKEFLEIREIYDLKPLENKNH"
     gene            7455..7964
                     /locus_tag="PLF66_21715"
     CDS             7455..7964
                     /locus_tag="PLF66_21715"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HMZ66720.1"
                     /translation="MENFKFRKAKQTDAVKILEIRKEAILKECIHVYPEDIITKWAEI
                     EITDSFLEDLSNDFYLVETRNQIVGTGMIDFSKSRIDAIFVSPSSMRKGVATSMLNYL
                     EKLAREKNLNYLKLESTLNAAPFYRLHGFVGERIQIYKSPRGFHLKCIPMIKELESEY
                     IPNVKSDFP"
     gene            8122..8535
                     /locus_tag="PLF66_21720"
     CDS             8122..8535
                     /locus_tag="PLF66_21720"
                     /inference="COORDINATES: protein motif:HMM:NF019013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HMZ66721.1"
                     /translation="MSNPSPKEIIHQWIEAYNSHNPEQASSLYDLNVINTQFPWGKSV
                     QGKDAMRNTYINIFKAFPDIHIQAENILETKDWAIVEWRFSGTMKGDFAGHPPNHNVF
                     DMNGCEMFQIANGKIIIQHGYWDKATMFTQLKIPL"
     gene            complement(8530..10764)
                     /locus_tag="PLF66_21725"
     CDS             complement(8530..10764)
                     /locus_tag="PLF66_21725"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HMZ66722.1"
                     /translation="MNPNPCDLEPIHLINELQDFGLIFIFSMHEDKIVATGENIFDYL
                     EISPEEAFTISLDRIFPDYENLKKLLIEHLIEKKHLEYKLLFPFSTSFFVAVFRKSDD
                     FFICELEREDSNQILFNTNNFFNQVQELNQIQTLNTFYQHIVNEVQKMTLFDRVVIYR
                     FDPEFNGEVLAESRKDDSIDSILGFHFPSSDIPLPARAMYKQNPLRMIQAHFDRKIIL
                     VKNKNYSNYSFDLGSSYLRTPHEHHLEYLKNMQIATSICVALSDGNRLWGLIICHSRE
                     KAIPLPSIRQNLILLSRIAYRHLDLLIQNENSQTIIKLKDELMEIIGKIYQISFTEIA
                     IIFEFNLDKLMTTFQADGAIILFGDVKIHRGEIPDPENFQKLISVLEEKSDSENFLTN
                     DIKDLFLEGSLDSNLRGGLFSFPISSKKKDRIYFFKKDALQKIVWAGNKNEAFLREEN
                     RISPRKSFTRWIEIVKGKSTNWNFSLRDIKEELQGIKDVIDKKLTKQTLIETLEKVSS
                     SEAKLKELNATKDKFFSILSHDLRNPISGIAGIAEILIDLSKEKELDLPQIEEYANLL
                     QQCSIKTIDLMQNLFLWGKIQTGKISIEKKNCSVSILFEDLEYFLSQRLKEKKIHLKS
                     EFDSSLSIFTDEKAIQTILLNILFNAIKYSFESATIYIRLAKNQNQVNIEIEDSGIGI
                     KEKDLEKLFRIGSNFKMPGTKQEDGTGLGLIIAKEYLNRLNGEIEISSTEGKGTKVKI
                     LLFL"
     gene            complement(10761..12005)
                     /locus_tag="PLF66_21730"
     CDS             complement(10761..12005)
                     /locus_tag="PLF66_21730"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HMZ66723.1"
                     /translation="MEYKILIIEDEAPARNYLSRTITDLGYKVNTAINGLEGLESFKN
                     DPTQIIITDLNMPVMNGYELIENINSFTDMVLPAILVVTSINDIQNVVDIMRKGAFDY
                     ILKPSNSNEPTIKIKNALQIYELNKIKYTIEKEKELRLKEHLDWNRYKENVIEKDFKN
                     FGSNLLQAMRHNFGQAGGLGSLLTLVELIDQTSILEGENYLISKDIIQLVKENAKILE
                     KSLLTINNISHIIENGFPLEKISLQEFYEIVIECVRKMDPFTSLHSNKILLSESKSNF
                     KDFFIMAERERLKEAIFELFKNACKFSEPDSNIYVLFTLTLNTLNFSILNEPDKRLTV
                     RGIPLEYSNLVFEPFFRISKFVEEQFETHDMGLGLSFVKAIIQKHNGLISISNIKDFT
                     AKNLDNIKVNLEINLPLHTEKT"
BASE COUNT         4464 a         1904 c         2232 g         4303 t
ORIGIN      
        1 ataagaaaat taaaaatcac atcttttcac aaatagcaaa agatgtgaaa aaaattagta
       61 aacgatagtt gcctatttta aatcagaagg cattctattt aagagtttat cgatttcctt
      121 ctttggaaat cctttcgcag agagaagctt tactgtattt gtaaatgttt cttttttcct
      181 tctttcattc cttttcttaa accctatctt aaaaccttct tctctgcctt cctcttttct
      241 ttttaaattc tgttgtttaa aaaaaagatc caatcttcta aaggtgattt gaaatctttt
      301 attttttttt ggatttggga tcactcaacg atagggataa ttagatcctt tgttacggga
      361 tttgaggtct attcttttga cttttggttc acccaaaccc aatcatttct tttgttcaga
      421 aatgacaata taagatgagt taataaattt ccaaaaaatt ttgtaagaaa tttgacgggg
      481 attcgcaagg gaaaaaaact gaaaaattat gaataaagaa gtaacagatc ctaaagtcat
      541 ccatcaatta attgattccc tttatagaat cactccaacc tatatggaag tagatggaga
      601 agatatacca gtcaaagtat tagacaatag acctcagtct gttacaattc ggaccccaaa
      661 ggttaattcc catacgagag aaagagttct tacgatgcgc agtaagggaa atattttcat
      721 cagcacattt ttagaagtgg gtatggatga tctagggaat tgtatcttac aacctttgaa
      781 acttttaatt aaagatgcgt taaaccaaga agatacaaac caatttattg tttcgaacgt
      841 gatcaatcaa aatgatattt ttaaatcttt gagtgatgat agaatcaatc aactgatcaa
      901 gaaagctcct aaagtaaatt tcatgtttga tttttttgaa gtgtatatca atgaaagaat
      961 gaatagtcgt atgagactca tgaatgctca tgaaaaacca atattcattc caaatatttc
     1021 acaacctgaa ttagttcctc aaaactttgt tccattcaat gaatacatcg ctctcacaaa
     1081 atcaagtaac gtcttagata aatataaatc agaaatttgc gttcctatta aatataaaaa
     1141 ttttatcatg attggctatg tgcatgcaat gcattccaca aggctagatt ctaattcatt
     1201 taatactttt aaattagttg catcctctat tatcaaagac atccactcaa ttggtgtttt
     1261 cgaagagtct cgtgagtctt gtaaggtgat tgaggcaaat cctggatcta ttcgttttct
     1321 gcatccaccg actagacttg caagtaaaat cttttctatg ggtgaagttg tgatctttga
     1381 tttgattgca aaatcaggtc atagaaagtt cgtaagaggt gtgatcaaag ggatcaaacc
     1441 aacacaaaaa gactatgcaa ttggttgtga cctatctagt aatacgccgg aagaaatagc
     1501 ggcattgact gagtttttag ggagacatta agattttaat gttgtaattt tattcatata
     1561 ctgcttaata cgataatcaa atagtagggg ttatatgaaa ataggattga ttggttcagg
     1621 tagttttgga acagctcttg gcagccttct tgcggataaa ggctatgatg ttttaatgtg
     1681 gactcgttct ccagaacagg cagaaggaat taatcttcat cacaaaaata agaaacatct
     1741 ttcggattta gttttaccag aaaatctaaa ggctactacc aatttggaag aggtagtaag
     1801 agacaaagat ttattattat cagcaccgcc gtcccatgta ttaacagaga ttatccacaa
     1861 gatcaaagat tatatttccc caaattgccc catcgtttct gccactaaag gtattgaaaa
     1921 tgaatctctt cgaatggttt ctgaaatatt tgagtctgaa cttcctggaa ggcttcataa
     1981 gaatctaagt tatctatcag gacctagttt tgctcgtgag attatttcta gagttccgac
     2041 agtcgtaagt attgcttcta aaaatgaagg aactgcaaag actgtgcaag agatatttag
     2101 cttcacttat tttagaacct attggactcc agatgttgta ggagttgaac taggtgggtc
     2161 attaaagaat gtaatagcaa ttgcagcagg agttgtggat ggacttggac aaggacaaaa
     2221 cacaagagca gcagttatta cacgggggct aaatgaaatt acccgcttag gagtaaaaaa
     2281 aggagctgac ccattgactt ttcttggtcc ttctggaatt ggagatttgg ttctcacttg
     2341 ctgtggagat ttgtctagaa atagaactgt tggtatgaaa ttaggacagg gaaaaacgtt
     2401 atctgaaata ttagcggaaa tgaatgaggt ggcagaaggt gtaaaaacta caaagagtgc
     2461 ctatgatctt tctaaaaaat taggagtatc tatgccaatt acagaagaag tttataaaat
     2521 gctttatgaa gaaaaatctc ccagagaagt tatgagagat ttaatgggaa gagatttaaa
     2581 aagagagggt atacattaaa atgatttata tagtaactgc acttgcgcaa gaagccattc
     2641 catttattca gcattttaaa ttaaaaaaag acttatctca tacaaagttt gatatttata
     2701 aaaatgaatt aatcactcta gttgtatccg gaacaggaaa actcaaatca tctatcgcta
     2761 ctacctattt actagtgaaa gaacctccta aaaaatcaga caagatttta aatttcggga
     2821 tttgtggttc tggctctgaa aaacacaaaa gtggacaaat atttttaatc aataaaattg
     2881 aagatgtaac gacggctaag acttattatc cagaaattct atttagtcat ttgatacctg
     2941 aagcgggaat tgtaactcat gaaaagccag tcgttaaaac aaaatctact ccttctacag
     3001 cgggtcttgt agacatggaa gtatctggtt tctttgaagc agccaatact tttgtccagt
     3061 cccataatat tatcattatg aaagttgttt cagatcattt gagtggagaa aagttcactg
     3121 gtgcatttat ccaaagcatt gttgaaaaaa atattcccca cgtaattaca gttctcaatc
     3181 aatctagaaa aaaacaatct actgctgttt ctgactctga aattttagat tcaaatgaat
     3241 atagaatttt tgaagattta agtgaagatc tagggcttag cacaacgcaa agattccaag
     3301 tattagatct aatcaaaggg tataaaatta gaggagaagg ggatttaaat tttttaaagg
     3361 attattcgaa atcaaaggca attcattcca agcaagatac caaaaaagct ttggaagaat
     3421 tgaaacaaaa attagataca agtggagaat agtttcttac taacttacct taagcaaaag
     3481 atacgaattg atacgtatga taaaagaaat tattatatta gatttttcta atgaatcaat
     3541 aaataagtgg gtttgggtga tagccccaag tgatagtcnn nnnnnnnnnn nnnnnnnnnn
     3601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3661 nnnnnnnnnn nnnnnnnntc cctttctctc actttcgcac gagcgttagc gattttttaa
     3721 agtgagatat aaaatgagtg aagcagaaga aaagatagat ttatttagtg cagaagaggc
     3781 agtgattgca caaggtaatt caattttaaa aaataaagaa tttacggata acccactttt
     3841 agaggaatac aaatccttac ttgagcacta tcgtaaactt ttaaatcagg ataaaaaact
     3901 catcaagatc agtgatggac tgcaaaataa agtaaaaaaa gcaaaaaacg aaatagaaaa
     3961 tctaaatgaa atatcaagac agattagtat gtcgcttgat tttgatatag ttttcggaag
     4021 tatatttgaa tatataaaag gtggttatgg attcacaggt tgttgtctaa cacttgttag
     4081 ccaagataaa tccaaatgta aaaatgaaaa attcatttct acttcagagt ttgactcgct
     4141 tgtagaaact tatacaggta gaaattatca attgaaattt ggaaatggtc ctattacaga
     4201 ttgtatttta aaaaatgaag tagggatcta tcatgatcaa aatatttttg atatggaaga
     4261 tagttctcac gctgtaaaaa atgcattttt aatgccgatt agcattgcag gtgaagtgat
     4321 tggagcattt agtttattag ataattcagg tatcaattta aaatcagatg aaaaggaatc
     4381 cttaaagaaa tttgcaaatc atatttctgt tgtgatcaag aactctaaga tttatgaaga
     4441 ttcagaaaaa gcgcgaatca aacaattaga agagtttaat atgaatttaa ctctaatcaa
     4501 taaatctctg caaaaatttg tgccaaaaga ttttgtgcaa tttttagaca aagaaaatat
     4561 aacggaagtg cgacttggag attcagtgca acaaaatatg tcgattctgt tttcagatat
     4621 tcgatctttt acaacgattt ctgagtcgat gtcacctaaa gataattttg gatttttgaa
     4681 ttcctattta aaagtaacag gtcctgtgat tcgaaagaac aagggcttta tcgataaata
     4741 cattggggat gctattatgg cactcttccc tgactccgcg tctgacgctg tgaatgcagg
     4801 tatagagatg ttagatgaag tccataaact aaatgtaaag agaaaagagt ggggcaggct
     4861 accaattcaa atcggaatag gaattcatac aggtgctcca atgctcggaa tcatcggaga
     4921 agaaaaacga tttgaattta cagtgatttc cgatgcagta aatcttgcct ctcgaataga
     4981 aggtcttaca aaagcatatt ccgcttctat cttaatttca gaagatacgt tagctgctct
     5041 tcaaaatcct gataaatata tctatcgaat ggtagacaaa gtaaaagtaa agggcaaaaa
     5101 caatccggtc attatctatg aaattttaaa tggtaactca gaaagaatta ttaatttaaa
     5161 actttctact aagaaaaagt ttgaagaagg tgttgaactt tatttagaag aaaagtttga
     5221 tgacgcctct atcctcttta aagaaatttt aaaaatagat ccaaaagata cggcagtctc
     5281 cctttattta gaacgagcca aatattatag tgtgcatggt gtccccctta attgggaagg
     5341 cgcttggaaa ttggatagta agtagaattt ttttgtcaga taactctttt tgtaaagaaa
     5401 ctcctaatta gattttgaat tgagcttctc taaaagagaa gatgtttgaa ttattggaat
     5461 agaatcgttt caaattgtcg atagttgtaa tgtatgcata taggtaaagc ttatacattt
     5521 aaagaagtca ttaattggac acgcagagac attttgcaat taggaattat ttctactata
     5581 ccagttattt tatacattca atttcagtgg aaatggttac agcttccatg gttgccgatc
     5641 gctcttatcg gtacagcagt tgcctttgta gttggtttta aaaataatgc agcatatgat
     5701 agattatggg aagctagaag aatttgggga tctattacta acttaagccg tagctgggga
     5761 gtaatggttc gagactatgt gacgaatcag cattctaaaa atccgttatc cgacacagat
     5821 ttgagcaaaa ttcatttaga actttataat cgtcattttg cgtggctcac ggcaatgcgt
     5881 tttcaactac gtgaaaataa gccatgggaa gcaatcaatc taaagcacaa tctagaatat
     5941 agaaatacgt attttacagt acaagagcat actcaagatg taaaagatgt tttgaagaaa
     6001 tatttaacag aagctgaatt caataaagtg attggcaaaa caaataaagc ttcttatatt
     6061 ttggcattac aatcagaaca cttaaagaga ttatttgaat taggattgat tgataatttt
     6121 agacatatgg aattggaaaa aatattgata gaatgttaca atcaacaggg aggaagtgaa
     6181 cgtattaaaa actttccata ccctagacac tttgcaagtc ttaacgcttg gtttattaag
     6241 atttttattt ttctaatgcc ttttggtatg atagaggaat tcgaaaaatt gggaccagaa
     6301 tttgtatggt tgaccatacc ttttgcatcc ttagccggat ggatttttat tacaatggaa
     6361 cgaattggag aatctacaga aaaccctttt gaagggagtg cgaatgatgt tccaatttct
     6421 acaatcagtc ataatattga aattgacatg ttggaactac ttgatttaca acataatcta
     6481 aaatcaattt cagcacaaaa taatatttta acataatgaa attcgaaact atatttaaaa
     6541 acaatgagaa atgggtagag gcaaaattaa aactcgatcc acagtatttt gaaaagctat
     6601 ctaaaggaca agctcctgag tttttataca ttggatgtag cgatagccgc gttactgcag
     6661 aagatttgat gggagtaaat ccaggtgaag tttttattca tcgaaatatt gccaatcaag
     6721 tagttcctac ggataataat atcaattcag ttattcaata tgcagtggag catttgaaag
     6781 ttaagcacat cattatttgt ggacattacg aatgtggtgg agtaaaagct gctttacatc
     6841 caagtgatat gggacaactc aatagttggt tgcagacgtt acgcgatgta agaagaattc
     6901 atagggcaga attggaacaa attaaggatg aacaagaatt atttgataga ttggttgaac
     6961 tcaacgtaag agagcaatgt atgaatatta ttaaaattga tcatgttcaa cgcagttggt
     7021 ataaaacagg atttccaaag attcatggtt ggatttttga tgtaagaact ggtaaactta
     7081 aagattccgg tttaaatttt gaaaaagaat ttttagagat aagagaaatt tacgatctaa
     7141 agccattaga aaataagaat cattgagatt ggaaattacc gcagagtaga aaataaaaaa
     7201 tattaccttg attgaattcg aatgttctaa cttagaaaag gatcgaagca aaaaaggaaa
     7261 aattagagtc tcctagtttt tttgatttta ttgaaataat taagcctgaa gagcattttg
     7321 ctaaaaatga ggattatgat cctttaactc atctattttc atgactagat gtttatcttt
     7381 tatctgaaga ggatattaga aatcgtttta agataaatta ctaaagatat aatagaaagt
     7441 aagaagaatc cttagtggaa aattttaaat ttagaaaagc aaaacaaact gatgcagtta
     7501 aaatactcga aatccgtaaa gaagcaattc taaaagaatg catccatgta tatccagaag
     7561 atataattac gaaatgggca gagatagaaa ttacagattc ttttttagaa gatttatcaa
     7621 atgattttta tttagtcgaa actagaaacc aaatcgtagg gactgggatg attgattttt
     7681 ccaaatcccg tatcgatgca atttttgtat ccccttcctc tatgagaaaa ggcgttgcaa
     7741 ctagcatgtt aaactattta gagaaacttg ctagagagaa gaatttgaat tatttaaaat
     7801 tggaatctac tcttaatgcg gctccattct acaggcttca tggatttgtt ggggaaagaa
     7861 tacaaatcta taaaagccca agaggttttc atttaaaatg cattcctatg ataaaagaat
     7921 tggaatcaga atatatacct aacgttaaaa gtgatttccc ttaatgaaaa attagtaaag
     7981 aattttgatt tataattctc tctaaaaata ccctatgaaa taattagtgt cttttgcttc
     8041 ctcttttata gaatggtaga ataaaaaaaa taaaaattgc agaaacaaga tagaatacta
     8101 ataaacaaag gaatcaaacc aatgtcaaat ccatctccta aagaaataat ccaccaatgg
     8161 atcgaagcgt ataatagcca taatccagaa caagcatcat ctctttatga tttaaatgta
     8221 atcaacactc aattcccttg gggtaaatct gtccaaggaa aagacgctat gcgcaatacc
     8281 tatataaata tttttaaggc attccctgat attcatatac aagcagagaa tattttagaa
     8341 acaaaagatt gggctattgt ggaatggcga tttagtggaa caatgaaagg agattttgca
     8401 ggtcatcctc caaatcataa cgtttttgac atgaatggtt gtgaaatgtt tcaaatagca
     8461 aatggaaaaa ttataattca acatggctat tgggataaag caactatgtt tactcaattg
     8521 aaaatcccat tataaaaata aaagaatttt tacttttgtt cctttccctt ctgtgctaga
     8581 aatttcaatt tctccattca gccgattgag atattctttc gcaatgatta aacctagacc
     8641 ggttccatct tcttgtttag ttccgggcat tttaaaattt gaacctattc taaaaagttt
     8701 ttcgagatct ttttctttta taccaattcc agaatcttct atttctatat tcacttggtt
     8761 ttgattttta gctaatcgaa tataaattgt agccgattca aaggaatact taattgcatt
     8821 gaataatata ttgagtagaa ttgtttgaat tgctttttca tccgtaaaaa tcgaaagaga
     8881 agaatcaaat tcagatttaa gatggatctt cttttctttt agtctttgag ataaaaaata
     8941 ttctaaatct tcaaatagaa tagaaacact acagtttttt ttctcaatag aaatctttcc
     9001 agtttggatt tttccccaaa gaaataagtt ttgcataaga tcgattgttt tgattgaaca
     9061 ttgttgaagt agatttgcat attcttctat ttgaggaagg tctaattctt tttccttgga
     9121 gagatcaatt aatatctctg cgattcctgc tattccagaa atcggatttc ttagatcatg
     9181 agaaagaata gaaaaaaatt tatctttggt tgcgtttaat tctttgagtt ttgcttcact
     9241 agaagatact ttttctaatg tctctatcaa ggtctgtttg gttaatttct tatcgattac
     9301 atcctttatc ccttgcagtt cttcttttat atccctcaaa gaaaaattcc aattggttga
     9361 ttttcctttt actatttcga tccatcttgt aaaagatttc ctaggagaga tgcgattttc
     9421 ttcgcgtaaa aatgcttcgt ttttattgcc tgcccaaaca attttttgga gagcatcttt
     9481 tttgaaaaaa tagattctat cttttttttt agaagaaatt ggaaaggaaa ataaacctcc
     9541 tcgtagattt gaatctaagg agccttctaa aaataaatct tttatatcat tggttagaaa
     9601 attttcgctg tcagattttt cttctaatac agaaattaat ttttgaaaat tttcgggatc
     9661 aggaatttct cctctatgaa tttttacatc tccaaagagg atgattgctc catcagcttg
     9721 aaatgtggtc attaatttat ctagattgaa ttcgaatatg attgcaattt cagtaaatga
     9781 aatctgatat atttttccta tgatttccat taattcatct tttaatttga taattgtctg
     9841 agaattttca ttttgaatca ataaatcgag gtgacgatag gcaatcctag ataataaaat
     9901 taaattttgt ctaatagaag gcagagggat tgctttctct ctagaatggc aaatgatcaa
     9961 accccaaagt ctatttccat cagaaagagc gacacatatc gatgtggcaa tttgcatatt
    10021 ctttagatat tccaaatggt gttcgtgtgg agttcttaga taagatgaac ctagatcaaa
    10081 agaataatta gaatagtttt tatttttaac taaaataatt ttcctatcga aatgagcttg
    10141 gatcattcta agaggatttt gtttgtacat tgccctcgca ggaagtggta tatccgatga
    10201 tgggaaatga aatcccagta ttgaatcaat actatcgtct tttcttgatt ctgccaatac
    10261 ttctccattg aactctggat cgaatcgata gatgaccact ctatcaaata aagtcatttt
    10321 ctgaacttca tttactatat gctgataaaa agtatttaga gtttggattt gatttaattc
    10381 ttgaacttga ttaaagaaat tatttgtatt gaataaaatt tgatttgagt cttctctttc
    10441 taattcacaa ataaaaaaat catcgctctt tctaaatact gcaacaaaga aagaggtgga
    10501 aaaaggaaaa agtaatttat attcaagatg ctttttctca atgagatgct ctattaataa
    10561 tttttttaaa ttctcataat caggaaaaat tctatctaga gaaatagtaa atgcttcttc
    10621 aggagatatt tctaaataat caaagatatt ttcacctgta gctacaatct tatcctcatg
    10681 cattgaaaaa ataaatataa gtccaaaatc ttgtaattca ttgattaaat gaattggctc
    10741 taaatcacaa ggatttgggt tcatgttttt tctgtatgta aaggcaaatt gatttctaga
    10801 tttactttta tattatctag attttttgca gtaaagtctt tgatgttgga aatactgatg
    10861 agcccattgt gtttctgaat gattgctttc acaaaactga gtcccaaacc catatcatga
    10921 gtttcaaact gttcctctac aaactttgaa attctaaaaa atggttcaaa tactaaatta
    10981 gaatattcta aaggaatacc acgcactgta agtcttttat ctggttcatt cagtatagaa
    11041 aagtttaatg tattgagcgt tagtgtaaat aaaacatata tatttgaatc tggctcagaa
    11101 aatttacatg catttttgaa aagttcaaag attgcttctt tcagtctttc tctttctgcc
    11161 attataaaaa aatctttgaa attggattta gattcggata aaagaatttt attagaatgc
    11221 aaagatgtaa atggatccat tttccttaca cactctatca cgatttcata gaattcttgt
    11281 agagagattt tttctaatgg aaatccattt tctatgatat gggaaatatt attaatagta
    11341 agaagtgatt tttctaaaat cttagcattt tcttttacta attgtataat atcttttgaa
    11401 attagataat tttcaccttc taaaatagat gtctgatcga tcaattcaac taacgtcaat
    11461 agagaaccga gtccacctgc ttgcccaaaa ttatgacgca tcgcctgaag taagttactt
    11521 ccaaagtttt taaagtcttt ctcaattacg ttttctttgt atcggttcca atctaaatgt
    11581 tcttttagtc tcagttcctt ttctttttct attgtatatt ttattttatt cagttcgtag
    11641 atttgtagtg cattctttat tttgatagta ggttcatttg aattgcttgg ctttaggata
    11701 tagtcgaagg ctccctttct cataatatct actacatttt gaatatcatt tatgctcgta
    11761 actacaagga tagcaggcaa gaccatatca gtgaatgaat tgatgttttc tattaattca
    11821 tacccattca taactggcat atttaaatct gtgataatga tttgagttgg atcattttta
    11881 aaactttcta atccttccaa tccattgatt gctgtgttta ccttataacc taaatcggtt
    11941 attgttcgac ttaagtagtt tctagcggga gcttcatctt caataattaa gattttatat
    12001 tccatgattg ggtatccgtt ttattcatta gaaaatcttt gaaatgatgt atttaaatta
    12061 gagtgtgaat tctcttcgtg cttatttttt agttctacta aatttctttc caatgcggaa
    12121 ttaaatctca tctaatttct gcttcatgcc aaagtaaagt gagttcattt tcaataccca
    12181 tctagctttt tgtaaagccg gtcaatgaat tgcacaattg aattacaaaa gggattctta
    12241 tctaattttc ctttacacta cttgccatcg gcaacttaat tactgttaaa gactctgtat
    12301 tttattgtga aacttcaagg attcattcag ggaatattct ctttctgatc tatgtctcgt
    12361 ccacgtaaaa acgaagggaa aaaatgtcaa tagtcttcgt gaagttgagg tctcggggaa
    12421 aacatttgat tagccgctct ttcgtaatga atgaactttt cgtttttaag aatgattcga
    12481 ttaacaaaat gcataaagaa tcaaatagtt ttggattttt gaaataccta taagtctctc
    12541 tattctattc atctgaagaa ataaatatat tttttatttt taacgttgcc cttcactcaa
    12601 ccgcaatctt ctaaatcact aagtggataa cacttatttg cctttataga agatgaagga
    12661 agagtagtcc gtgctcagtc ctctctaagt tctaagattt agagaccaaa tccttagtat
    12721 gttcagaata ttaacagaag attgtttttt gggaaaaaga gaagctcgaa attacctagg
    12781 gcgggggtcg aacccatatc tgagcgcccc aaggaagcga agatgggctt tcatgagcag
    12841 agcgaaagga aagagaaggg ggagattgct cgaaattacc tagggcgggg gtcgaaccca
    12901 tatctgagcg ccccaaggaa gcgaagatgg gctttcatga gcagagcgaa aggaaagaga
    12961 agggggagat tgctcgaaat tacctagggc gggggtcgaa ccc
//
LOCUS       DAOQOS010000139        12844 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_2511_length_12844_cov_6.496286, whole genome shotgun sequence.
ACCESSION   DAOQOS010000139 DAOQOS010000000
VERSION     DAOQOS010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 12844)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12844)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12844
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2511_length_12844_cov_6.496286"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            55..285
                     /gene="vapB"
                     /locus_tag="PLF66_21735"
     CDS             55..285
                     /gene="vapB"
                     /locus_tag="PLF66_21735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002978019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HMZ66724.1"
                     /translation="MNRAKLFKNGDSQAIRLPKEFRFKGKEVYIRRDGNNIILTPIDD
                     AVDRLWNSLSSFSEDFKIERNQPKAHDKRNPL"
     gene            282..686
                     /locus_tag="PLF66_21740"
     CDS             282..686
                     /locus_tag="PLF66_21740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012390319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HMZ66725.1"
                     /translation="MNQYLLDTNICIYIINKRPDSVYKKFKKINLDNIFISVITDFEL
                     RYGVEKSQRSEKNFQILEEFLGYLNVLPLETSDSMIAGALKNRLEKKGELIGPYDLLI
                     ASQAISRDLVLVTNNEKEFKRIKEIKIENWIS"
     gene            complement(688..1092)
                     /locus_tag="PLF66_21745"
     CDS             complement(688..1092)
                     /locus_tag="PLF66_21745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002774721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2721 domain-containing protein"
                     /protein_id="HMZ66726.1"
                     /translation="MELTLNTPSLLFPAISLMMLAYTNRFLSLSKLVRDLYNEYSTTK
                     EQKVLVQIRSLRLRLRLIRYMQFFGVSSILLCVICMFFIFIEELGLAKYIFITSLILL
                     AISLTFSVWEIQISTKALSVELSDLEELGEKK"
     gene            1222..2451
                     /locus_tag="PLF66_21750"
     CDS             1222..2451
                     /locus_tag="PLF66_21750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010416411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 family protein"
                     /protein_id="HMZ66727.1"
                     /translation="MQWLNELVQTHPDLIKFISIPITCAFVGWITNYVAVKMIFHPAE
                     FWGIGKLGWKGIIPNHAIKMSGLIAKILTERLVKPHELFLRVNPDEINHEIQDLIDLK
                     AKEIIKDIIATENPVLWSLLSDDIKNSIEEEVRREIPKQIVDIYKSFGNELDNVLEFD
                     EIIRSSLSGKKTSILIEMFQRCGGPEFRFIIVSGIYFGFLIGLIQLAFINILGQWWTM
                     PIMGVVVGYYTNWIAIQMIFKPLEPKKFFFFFTYQGLFLKRQEVVSKEFANVVANNVL
                     NQENIIRLIFTGKGGDLLIKLVIEKAYKLTHQKMMERAPLMPVLLGSEKIRQIKERIA
                     EKLIWILPDVANRIQKYLNDRLQIEKTIAERLSVLPKAEFEELLHSVFKEDELTLIIL
                     GAILGGLVGLYQAYLVF"
     gene            complement(2533..3048)
                     /locus_tag="PLF66_21755"
     CDS             complement(2533..3048)
                     /locus_tag="PLF66_21755"
                     /inference="COORDINATES: protein motif:HMM:NF019235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1564 family protein"
                     /protein_id="HMZ66728.1"
                     /translation="MKTKIISNQKLSFPRNHYQESDFSSTLLVPEKYITLFYQKISHH
                     KGVRSYIAYLLNKYQIYIANGLIPSYCKVTTKYQEKGQNLQRIAFRPKPEDWAELKLY
                     RLSFGMSISALVVYLLIADSVDFAETFSNYLTSVGISPIPQFDLFAKVQLCNKRSDYT
                     IIFQYRKGHYH"
     gene            complement(3155..3772)
                     /locus_tag="PLF66_21760"
     CDS             complement(3155..3772)
                     /locus_tag="PLF66_21760"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HMZ66729.1"
                     /translation="MNKVIFEKYLNREKSLKINFDLDFENEIIALLGKSGAGKTTILR
                     CIAGLTNPENGFIKIKNETWYDKKKSINLTPQKRDVGFVFQDYALFPNMTVANNLEFA
                     LKDKSDFNWYHKIIDIARIESFLHRKPDSLSGGEKQRVAFARAVIRRPKILLMDEPLS
                     ALDYEARKSLQDEILFCHIEMQIPILWVSHDLTEIQKISSRIIKI"
     gene            complement(3781..4452)
                     /gene="modB"
                     /locus_tag="PLF66_21765"
     CDS             complement(3781..4452)
                     /gene="modB"
                     /locus_tag="PLF66_21765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016545326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HMZ66730.1"
                     /translation="MNYDLTPFILSIKLASLTTIILFLIGIPISYYLAFTDSKFKIFV
                     NTIITLPLVLPPSVIGFYFLLLFSPQNKIGQILEKFFNLRFVFSFEGLLLGSILFGLP
                     FMINPIFSSFERFPITLQEAAFTMGKSKIETLIKIIIPYSKAAILNGILICFAHTIGE
                     FGLVLMIGGSIPGKTKLASISIYQEVELMNYNSAHFQSGLLLVLSFILIFLIQITQYK
                     SKPRF"
     gene            complement(4442..5194)
                     /gene="modA"
                     /locus_tag="PLF66_21770"
     CDS             complement(4442..5194)
                     /gene="modA"
                     /locus_tag="PLF66_21770"
                     /inference="COORDINATES: protein motif:HMM:NF024921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HMZ66731.1"
                     /translation="MKYLLLIINFLISISIYSEEFIVAGAANLQYALKALQLEYNQEY
                     SKNIQIVLASSGKIFTQIQNGAPYSIFLSADMNYPSKLYKSGHSIAEPTIYAKGTIVL
                     WSSNRMIDKDIKKILLNPKNKTIAIANPETAPYGKESINILENLKIYNDIKSKLVFAE
                     SISQVNQYIFSGAAEIGFTAKSIVTTKNNINKENCVEIDKGLYKPIDQGIIILKYGLD
                     HFPEESKSFYNFIFSKKGKYILSEYGYIINEL"
     gene            complement(5191..6570)
                     /locus_tag="PLF66_21775"
     CDS             complement(5191..6570)
                     /locus_tag="PLF66_21775"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HMZ66732.1"
                     /translation="MKLTRSKFLKNTAITFTALSAVKDLFSQTNLLSQKKQSNGIKNI
                     TIIGAGLSGLYSAYLLKNAGYEVTIIEAKNRIGGRVLSYVDEETKLITELGGEWIQEN
                     HYTIKSLCKELEIELKTIPTAKDILIGDKFIEANLLKQSPETKEILKRVFSLYEKMSA
                     EKKQGLDKLDIYSFLKYQGVNEEELYHLEMKYSIYFGESLRTLSAEKAIPYLDFYENS
                     FPFQYKINGGSEKLVETLKTKLKGVTVSLADPVVSIEDTSTSTKVKLKSGKELISDAC
                     ILSIPVTQLNGIRFLPDLPKDKKLAILQSKMSRMTKGVAIYKGIDYVRDKLFIQSDGI
                     FQSIYSLGNKNKTLNKGTLSLIATGDRSDVCSRLSSEYQGSFVKNNLDNIELLSTLKF
                     EKIQMKSWQNESFIEGATSYYMPGTYDVKNILRKNHGRVFFSGEHLSIQSGTMEGALL
                     SAIEVVNSL"
     gene            complement(6579..7889)
                     /locus_tag="PLF66_21780"
     CDS             complement(6579..7889)
                     /locus_tag="PLF66_21780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66733.1"
                     /translation="MQKLLYLLVLLLATNNVFAEKVITAKEDLPDAYFGLKVGAVISP
                     AFGYRLRDSSSGTSNAKKDDRTGFSMPWTLFMISKEWEEKKVTVEFWGELIRSSVVSS
                     DTTADNGNKSNPYILGIRRASVKKNFELGIFNFSLIFGIHELPHVYTQWNGYWKWRYV
                     DRAPMESLGFSPQPADLGASFLMQAKGFSAHIGIVNGEGYRDLQNSNSSGYDAVSRFS
                     YEQEIQKFKIGFHLLGRAGNVAGVAGDECREGKTNCLASDNKNSTMIVRDLRYQKSQT
                     LGAEYTMLFDRYLNLGLGFVHRKIFQGLTYDALNLSTYPIYLSDIGKTAYYLWIGGGY
                     GDFNLIARTERGSGSNGVLGNSAGGFIFTKNSFFIEYLYSESVRFAFGGSILMQGSKV
                     YIDSVGNTRTQTDYLNQFYRGQNLGIVEYSNNDRQLFIRSTMDF"
     gene            complement(7894..8316)
                     /locus_tag="PLF66_21785"
     CDS             complement(7894..8316)
                     /locus_tag="PLF66_21785"
                     /inference="COORDINATES: protein motif:HMM:NF025473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABA4-like family protein"
                     /protein_id="HMZ66734.1"
                     /translation="MTPEQVFKIANAVAIIPWILMTVAPRWNITEKIINSYSFPALLA
                     CVYIFYILISLGRAEGGFSTLDGVASLFKNKNALLAGWVHYLVFDLFVGTWEWRDSIQ
                     NNISHFILVPCLFLTLMFGPAGFLIYYLIRLFLVGSLI"
     gene            8557..9318
                     /locus_tag="PLF66_21790"
     CDS             8557..9318
                     /locus_tag="PLF66_21790"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HMZ66735.1"
                     /translation="MKNIAHRGFSSKFPENTMLSFQKAIEISADWIELDVTLSKDNIP
                     VVIHDDTLDRTSNGKGLVQKYSLKQLKTFDFGSWKNRRFAEEKIPTLEEVLKLIHNQS
                     IHLNIEIKSSAYKKKDSSHSIERQVLFLVKKYKLMGRVVISSFEPKVLTRLRSLSTKI
                     RLAYLIEPGYEKIMPDPISFVKEIKADSLNIHKSQIRSRLFQKIRAEKIPTYVHTVNT
                     LVEFRKMIGLGIAGVFTNYPDRLKLILNSSESISK"
     gene            9477..11813
                     /locus_tag="PLF66_21795"
     CDS             9477..11813
                     /locus_tag="PLF66_21795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66736.1"
                     /translation="MKNINTILIILLLTSNCLKTKRAAFDLTSPAGLINFSFANFAFG
                     QSQGLGFTVGGSIQGFTSGTLILKNGQDQLTLSPITIYSFRVASGATYSIQVQENPSE
                     FTCSIANASGTANANISNVNISCTALPSISALYPNNGKSWNSYVKNDGTGRFQATDTA
                     CVSSVSGGYNACLHGGEMRKIDLSDRTSCSNLTASDNVNALNWICRTTSSGIQFVSTG
                     LKTGKGLSDLIDWTLSSPSILPMNLTIKEGSNTLYQTTSSVWWDNPVVVVSPSAVNTA
                     ITTSGTIYLLTSSTPISDAGNIPQVSIGVSNVAFLIKPGLRMTATAPACVTSRYMIDV
                     VNASFSWVEGDFDASLHPGGITLRSNSYFSVIRNIKVQNTGINPLCGAANSTFASVTI
                     QTFNNYISNVTIANSNPTGFLLNTANAKENLIYNISIFNSQDVTFGVGLRLDTNVTKN
                     FISGVLVANNASDGVSIVNSSVNNYIFNISSVNNNGIGEKLNSSNTLLTNVMNLNNGN
                     DHLQMNGAPTSTLQNYVSGATPNSHVNLSGGNTDTYFTGIFKVNSVACITAGGSSGGI
                     TGTCNGAVTGTRPSDINYPSTNSFLNFSATPLNQIFIGQASSESANSYYASGSNTYST
                     SVSWTSFQNFYRNLGKSGTFAASSSRGACIAGGVCQIYDWTFKTTDTIGLNTLPCPGN
                     TAQLPATVVTHPLNGGGSVTFLRNAVEIIGDGIGDDNGFCQSFEACLYTPNVGSYQGH
                     GNLVSASVAVANTSTCSDIGSTGPVTNITLYKYDTNGY"
     gene            complement(11818..12765)
                     /locus_tag="PLF66_21800"
     CDS             complement(11818..12765)
                     /locus_tag="PLF66_21800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020783383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMZ66737.1"
                     /translation="MIEVKNLYSSYKVNSKEFFKKNIIKAVEDVSLSIPKNHTLGLVG
                     ESGCGKSSLGRTILRLQPFESGNILYKDKDLTLLSQRQLLPLRKDLQIIFQDPYSSLN
                     PRLTIFEIITEGLFVHKKAEKKEALELSIQMLEKMSLKPDILNRYPHEFSGGQRQRIA
                     IARALILNPEFIVCDEIVSALDVSNQAQVINLLSDYKKDKNLSLLFISHDLNVITHIS
                     DDIAIMYLGKIVEMGSKKEIIEKPSHPYTKSLFASTFELSNRKKKRIILSGEIPGVLN
                     KPSGCYFHTRCPIAQDICKKEIPPSKKLSPTHFSSCHFS"
BASE COUNT         4246 a         2076 c         2164 g         4358 t
ORIGIN      
        1 tttattaaaa tctattctgt aaaccctttt tcttatttcg caacagactg atatatgaac
       61 cgagcaaagt tatttaaaaa cggtgacagc caagcaattc gacttcctaa agaattcagg
      121 tttaagggaa aagaagtgta tattagaaga gacgggaata atattatttt aacccctatc
      181 gacgatgctg ttgacagact ttggaattca ctcagctcat tttcagaaga ttttaaaata
      241 gaaagaaacc agcctaaggc acacgacaaa cggaatcctc tttgaaccaa tatctcttag
      301 atacgaatat atgtatctat attattaata aaagacctga ctctgtttac aagaaattta
      361 agaaaataaa tttagataat atttttattt cagtgatcac agattttgaa ttaagatatg
      421 gtgtagaaaa aagtcaaaga tcggaaaaga actttcaaat actggaagag ttcttaggat
      481 atttaaatgt tctccctctt gaaacatcgg atagcatgat tgcgggtgct ttaaaaaatc
      541 gtttggagaa aaaaggggag ttaatcggtc cttatgatct tttgattgca tcgcaagcaa
      601 tttctagaga tttagtttta gttacaaaca atgaaaaaga atttaaaaga atcaaagaga
      661 ttaaaataga gaattggatt tcttaaatta cttcttttcg cctaactctt ctagatcgct
      721 tagttcaacg gataacgctt ttgtagatat ctgaatttcc caaacagaaa aagttaagga
      781 aattgcgagt aaaattaaac tcgtaataaa aatatatttt gcaagaccta gctcttctat
      841 aaaaataaaa aacatgcaaa tgacacagag taaaatactt gatactccaa agaattgcat
      901 atatcgaatt aatcttaatc gtaagcgcaa ggaacgaatc tgaactaaaa ctttttgctc
      961 ttttgtcgtg gagtattcat tgtataaatc tctgactagt tttgataaac ttaagaaacg
     1021 atttgtatat gctaacatca taagagatat tgcaggaaat agtaaagatg gggtattgag
     1081 tgttaattcc attctagcat gctcctgact ttaagtagat tagtcaactt gaaacaagcc
     1141 ttccttttag agtgcaaaat aaaatttgga ttgcataaaa aattgggaaa ctttttctat
     1201 ggtgagtgga gtaaccaaaa tatgcaatgg ctcaatgaat tagttcaaac tcatcctgat
     1261 ttaattaaat ttatttcaat cccgattact tgtgcgtttg tcggttggat tacaaattat
     1321 gttgcagtaa aaatgatttt tcatccggct gagttctggg gaatcggaaa gctcggatgg
     1381 aaaggaatca ttccgaatca tgccattaag atgagtggtc tcattgcaaa aattcttacc
     1441 gaacgtttgg ttaaacccca tgaattattt ttacgtgtaa atccggatga aattaatcat
     1501 gagatccaag accttattga tttgaaagca aaagagatta ttaaagatat cattgcaacc
     1561 gaaaacccag tcctttggag tcttttgtcc gacgatatta agaattctat cgaagaagaa
     1621 gttcgtcgtg aaataccaaa acaaatcgta gatatttaca aatcatttgg aaatgaattg
     1681 gacaatgtcc ttgaatttga cgagattatt cgttcctctc ttagcggtaa gaagacatcg
     1741 attctaattg aaatgtttca gagatgtggt ggtcctgagt ttagatttat aattgtatct
     1801 ggaatctatt tcggatttct aattggtctc attcaattag cctttataaa cattctaggt
     1861 caatggtgga ctatgccgat catgggagtg gttgttggtt actatacaaa ttggattgca
     1921 attcaaatga tttttaaacc acttgaacct aaaaagtttt tcttcttttt tacttaccaa
     1981 ggtctttttc tgaaaagaca agaagtggtt tcaaaggaat ttgcaaatgt tgtagcgaat
     2041 aacgttttga atcaagagaa tattattcga cttattttta caggtaaggg tggtgattta
     2101 ttaatcaaac ttgttattga aaaagcatat aagctaaccc atcaaaaaat gatggaacgt
     2161 gctcccctta tgcctgttct tttaggatcg gaaaaaattc gtcagatcaa agaaagaatc
     2221 gcagagaagc ttatttggat tttacccgat gtcgcaaatc gtatccaaaa atatttaaat
     2281 gatcgtttgc aaattgaaaa aacaatcgca gagagattat ctgttttacc caaagctgaa
     2341 ttcgaagagt tacttcactc tgtatttaaa gaagatgaac taacactgat tatccttgga
     2401 gccattttag gaggccttgt aggactctac caagcgtatt tggttttttg attcaccttt
     2461 ggatattcct agcttgttgt attctgtgag attttgttta atttgttagt tgttttagta
     2521 gattttattt tatcaatgat aatggccctt tcgatattga aaaatgattg tatagtctga
     2581 tcttttatta catagttgaa ctttggcgaa taagtcaaac tgtggaatcg gggaaattcc
     2641 tacactcgtt aagtagttgg agaaagtttc tgcaaaatca acagagtcag ctattagtaa
     2701 gtaaacgact aaggccgaga tagacatacc aaaacttaaa cgatagagtt ttagttcagc
     2761 ccaatcttct ggcttaggac gaaatgcaat tctttgcaaa ttttgacctt tctcctgata
     2821 cttagttgtt actttgcaat agctaggaat cagaccattt gcaatataaa tctggtattt
     2881 attcagaagg taggcaatgt aagatcggac acctttatga tgcgaaattt tttgatagaa
     2941 gagtgttatg tatttttctg gaacgagtaa agttgaactg aaatcagatt cttggtagtg
     3001 atttcgtgga aatgaaagtt tttggtttga tataattttt gttttcattc tataaaaagt
     3061 ctcctttagt aaaaaagatt taggtgggaa gattggattt aaaggtcttc taatagaata
     3121 ggagaaaaaa attctcaaat tgtgcagaga aaatttaaat ttttataatt ctagaagaaa
     3181 ttttttgaat ttcggtaaga tcatggctta cccacaaaat aggaatttgc atttctatat
     3241 gacagaaaag aatttcatct tgtaaagatt ttcttgcttc atagtccaat gcagagagtg
     3301 gttcatccat taataatatt tttggccggc ggataacggc tcttgcgaag gcgactcttt
     3361 gtttttcccc tccagaaaga ctatcgggtt tacgatgcaa gaaactttct atcctggcta
     3421 tatcaatgat cttatgatac cagttaaaat cacttttatc tttcaatgca aattctaaat
     3481 tattagctac agtcatattt ggaaaaagag cgtaatcttg aaaaacaaac ccaacgtctc
     3541 tcttttgtgg cgttagatta atcgattttt ttttgtcata ccaagtttca tttttaattt
     3601 taataaaacc attttctggg tttgttaatc ctgcaataca tcttagaatt gtagtttttc
     3661 ctgcacctga ttttcctaaa agtgcaatga tttcattttc aaaatctaaa tcaaaattta
     3721 tttttaaaga tttttctcta tttaaatact tttcaaaaat tactttgttc atctatcaag
     3781 ttagaatcta ggtttagatt tatattgtgt tatttgaatt agaaaaatta gaataaagga
     3841 caaaactagt aataaaccag attgaaaatg agcagaatta taattcatta attccacttc
     3901 ttgataaatg gagatggagg ctaatttcgt tttaccgggt atgctgccac ctatcattag
     3961 cactaagcca aattctccaa ttgtatgtgc gaaacagatt aaaatcccat ttaaaatggc
     4021 agccttagag taaggaatta tgattttaat taaggtttct atttttgatt tacccattgt
     4081 aaacgctgct tcttgaagcg ttataggaaa tctttcaaag cttgaaaata taggatttat
     4141 cataaaggga agaccgaata ggattgatcc aagcagcaga ccttcaaagg agaatacaaa
     4201 tcggagattg aagaattttt ccagtatttg accaatttta ttctgaggag aaaaaagaag
     4261 taaaaaatag aatccaataa cggaaggagg aagaactaaa ggtaaggtaa ttatcgtatt
     4321 tacaaagatt ttaaattttg aatcagtaaa agctaagtag taggaaatcg gaattcctat
     4381 taaaaaaagg ataatcgttg ttaggcttgc taattttata gaaagaataa atggagttaa
     4441 atcataattc atttatgata taaccatatt cacttaaaat gtattttccc ttttttgaga
     4501 agataaaatt ataaaagctt ttagattctt caggaaaatg atctagtcca tatttcaaaa
     4561 ttataattcc ctgatcgata ggtttgtata atcctttatc tatttccacg cagttttcct
     4621 tgttaatgtt attcttcgtt gtaacaatcg attttgcagt aaatccaatc tcggctgctc
     4681 ctgaaaaaat atattggttc acttgagaaa tactttcggc aaaaaccaat ttacttttaa
     4741 tatcattata tatttttaaa ttttctaaaa tatttatgga ttccttaccg taaggtgcag
     4801 tctcggggtt tgcaatcgca atcgttttgt ttttagggtt aagtagaatt tttttaatat
     4861 ccttatcaat cattctattg gaagaccata gaactatggt cccctttgcg tatatcgtag
     4921 gttctgctat cgagtgtcca gatttatata atttacttgg gtagttcata tccgctgata
     4981 aaaagataga atatggagcc ccattttgaa tttgtgtaaa aatctttcct gaagaagcta
     5041 aaacaatttg aatgttttta gaatattcct gattgtattc tagttgtaga gctttaagag
     5101 catactgtag atttgccgca cctgctacaa taaattcttc tgaataaata gatattgata
     5161 ttagaaaatt gattataagc agtaaatatt tcataaacta tttacaactt caattgctga
     5221 aagtaaggct ccttccattg ttccgctttg gatacttaaa tgctctccag aaaaaaatac
     5281 tctaccatga ttttttctta gaatattttt tacatcatag gttcctggca tataatagga
     5341 agtagctcct tcgataaaag attcattttg ccatgacttc atttgtattt tttcgaattt
     5401 cagtgtagag agtaattcaa tattatctaa attattttta acaaagcttc cctgatattc
     5461 tgacgatagg cgagagcaaa catcagatcg atctccggtt gcaatcagag ataaggttcc
     5521 tttgtttaat gttttatttt tatttccaag tgaatatatg ctttgaaata ttccgtcgct
     5581 ttgtataaat aatttatcac gaacataatc gattcctttg taaatggcaa cacccttagt
     5641 cattctactc attttagatt gtagaattgc taattttttg tctttaggta gatctggtaa
     5701 aaaacgaatt ccatttaatt gagttacagg aatagaaaga atacacgcat cagagattaa
     5761 ttctttacca gatttgagtt ttactttagt agaagtagat gtatcttcaa tggatactac
     5821 tggatcagcc agtgatacag taacaccttt aagctttgtt tttaaagttt ctactaattt
     5881 ttcagagcca ccatttattt tatattgaaa aggaaatgaa ttttcataga aatctaaata
     5941 aggaattgcc ttttcagcgg ataatgttcg taagctctca ccgaaataaa tactatactt
     6001 catctctagg tgatagagtt cttcttcgtt gactccttgg tatttgagaa atgaataaat
     6061 gtctaactta tctagtcctt gttttttttc tgctgacatt ttctcataca gtgagaaaac
     6121 tcttttaaga atttcctttg tttcaggact ttgcttcaat aaattagctt caatgaattt
     6181 atcgcctatc agaatatctt tcgcggttgg aattgttttt agttcaattt ctaattcctt
     6241 acataaactt ttaattgtat agtgattctc ttggatccat tctcctccaa gttctgttat
     6301 cagttttgtt tcctcatcta catacgatag cactcgtcca cctatacgat tttttgcttc
     6361 aatgattgtt acttcatacc cggcattctt tagtagataa gcagaatata aaccagagag
     6421 tccagctcca atgatagtga tattttttat tccattcgat tgtttctttt gagataaaag
     6481 attggtttgt gagaataaat cttttacagc ggataacgct gtgaatgtta tagctgtatt
     6541 ctttaaaaat tttgatctag ttaatttcat acttcctctt aaaaatccat tgtggatcta
     6601 ataaataatt gtctatcatt gtttgagtat tcaacgattc ctaaattttg tcctctataa
     6661 aattgattta aataatcggt ttgggttctt gtatttccca cagaatcgat ataaactttt
     6721 gaaccttgca ttaagataga tccaccgaat gcaaatctaa cagattcaga atacaaatac
     6781 tcaataaaaa aagaattttt ggtaaaaata aaaccgcctg ctgaatttcc taaaacacca
     6841 tttgaaccag aacctctctc ggttcgggca atcaaattaa aatctccata tccaccccct
     6901 atccataaat agtaagcagt tttaccaatg tcagataggt aaataggata agttgaaaga
     6961 tttaaagcgt catacgttaa accttgaaat attttcctat gcacaaaacc taatcccaag
     7021 tttaaatatc tatcaaagag cattgtatac tctgcaccta gagtttgtga cttttgatat
     7081 cttagatctc ttacaatcat tgtagaattt ttattgtcac ttgctaggca gtttgtcttg
     7141 ccttccctgc attcgtctcc tgcaacaccg gcaacatttc ctgccctacc aagcaaatga
     7201 aagcctattt taaatttttg aatttcctgt tcataagaaa agcggctaac agcatcgtat
     7261 ccggacgagt ttgagttttg taaatctcta taaccttccc catttactat gcctatatgt
     7321 gcgctaaatc cttttgcttg cattaaaaaa gatgctccta gatcagcagg ttgtggagaa
     7381 aaaccaagtg actccattgg agctctatct acatatcgcc atttccaata accattccat
     7441 tgtgtataaa catgtggtaa ttcatggatt ccaaaaataa gtgaaaaatt aaatatacct
     7501 aattcaaaat ttttctttac acttgcccga cgaattccaa gtatataggg attggatttg
     7561 ttaccgttat ccgctgtagt gtcggaactc actacactag aacgaattaa ctctccccaa
     7621 aattcaactg ttactttctt ttcttcccat tctttactta tcataaataa agtccatggc
     7681 atagaaaatc cagttctatc atcttttttt gcattggaag ttcctgaaga agaatctctc
     7741 aatctatatc caaatgcagg agaaattaca gcacctactt ttaaaccgaa gtatgcgtca
     7801 ggtaaatctt ctttcgcagt aattactttc tcggcaaata cgttgttagt tgctaaaagc
     7861 aacaccaata aataaagaag tttttgcatg aatctaaatc aaactaccga ctaggaataa
     7921 acgaattaag tagtagataa gaaaacctgc aggaccaaac atcaaagtta gaaataaaca
     7981 aggaacaagt atgaaatgag agatgttatt ttgaattgaa tctctccatt cccatgttcc
     8041 cacaaataaa tcaaagacta agtaatgaac ccatcctgca agcaaagcat ttttattttt
     8101 gaaaagagaa gctactccgt ctaacgttga aaatccgcct tctgctcgtc caagtgaaat
     8161 tagaatgtaa aaaatatata cacaagcaag taaagcaggg aaggaataag agttaattat
     8221 tttttcagtt atattccagc gaggagcgac ggtcatcaga atccagggga taatggctac
     8281 tgcatttgcg attttaaata cttgttccgg agtcatattt atcctttcat acgtattaac
     8341 tgatattgcg caaatttgag aatcttagag acaaaaaaga attctgataa tattagaaat
     8401 acaattgaat ctatgttatc aacagcctgc gaaggcaagc tttctgacaa gaatatctca
     8461 tttgaacgct tggctcacct tgtgtatggt gagataatat attcatgtaa agagtattga
     8521 ttaaataaac tattttttaa aattagtaga taaatcatga aaaatattgc acatagagga
     8581 tttagcagta aattccctga aaatacaatg ttgtcatttc aaaaggctat agaaatcagc
     8641 gctgattgga ttgaattgga tgttacactt tctaaagata atatacccgt tgtaatacat
     8701 gatgacactt tagacagaac aagtaatggc aagggactcg tgcaaaaata ttctctcaaa
     8761 caattaaaga catttgattt tggttcttgg aagaatagaa gatttgcaga agagaaaatt
     8821 ccaactttag aagaagtatt gaaattaatt cacaaccaat ccattcactt aaacatagaa
     8881 attaaatctt ctgcttataa gaaaaaagat tcgagtcatt ctatagaaag gcaagttttg
     8941 tttctcgtta aaaaatacaa actgatgggt cgagttgtaa tctcttcctt tgagccaaaa
     9001 gttttaacac gtttaagaag tttgtctaca aaaattagat tggcttattt aattgaacct
     9061 gggtatgaga aaataatgcc agatccaatt tctttcgtga aagaaatcaa agcagattct
     9121 ttgaacatac ataaatcaca gattcgatca aggctatttc aaaagataag agcagaaaaa
     9181 attcctacct atgtccacac agtgaataca cttgttgaat tcagaaaaat gattggtctt
     9241 ggtattgcgg gcgtctttac taattatcca gatcgattaa agctgatttt aaattccagc
     9301 gaatcgataa gtaaataaat tatttgttta tagaaaatca atgtttacct agttgttaaa
     9361 ttagataagt agaaagctat ttatctaaaa tgattctttt cgctttcact taaaaatata
     9421 aaatcgcgtt tagtttgtcc caggcacaaa ctacaatttt taaaaataga aaatatatga
     9481 aaaacataaa cacaatactt ataatattac ttttaacctc aaattgtctt aaaacaaaaa
     9541 gggcagcatt tgatttaact agcccagctg gattaattaa tttttctttt gcaaattttg
     9601 catttggtca aagccaagga ctaggattta cggttggtgg ttcaatacaa ggttttactt
     9661 caggaactct cattctaaaa aatggacaag accaactaac tttatctcct attacaatct
     9721 attcttttag agtagcgtca ggcgcaacat attcgattca agtgcaagaa aatccttccg
     9781 aattcacttg ttctattgca aatgcatctg gaacagctaa tgcaaacatt tcgaatgtga
     9841 atatttcttg cactgcgctt ccttccattt cagctttgta tccaaacaat ggaaagtctt
     9901 ggaatagtta tgtaaaaaat gatgggactg gtcgttttca ggcaacagat acagcttgtg
     9961 tatcatctgt atctggaggc tataatgcat gtctccatgg aggcgaaatg cggaaaatag
    10021 atttatcaga tagaacaagt tgttctaatc ttactgcttc agataatgtg aatgcactca
    10081 attggatttg tagaactact tctagcggca tacagttcgt atccactggc cttaagacag
    10141 gaaagggttt atctgattta atcgattgga cgctttcttc gccaagtatt cttcctatga
    10201 atctcaccat aaaagaagga agtaatactc tttatcaaac aacaagttcg gtttggtggg
    10261 acaatcctgt tgtagtagtt tctccttcag cagtgaatac agctattact acttccggaa
    10321 ctatctatct attaacgtct tctactccaa tatctgatgc tggaaatatc ccacaagtga
    10381 gtattggagt ttctaacgtt gcgttcttaa ttaaaccagg gcttagaatg acagctacag
    10441 caccggcgtg tgtaacttct agatatatga ttgatgtcgt aaatgcaagt tttagttggg
    10501 tagaaggtga ttttgatgcc tctctacatc caggaggaat aacgttaaga agtaactcct
    10561 atttttcagt gattcgaaat ataaaagttc agaatacagg catcaatcct ctttgtggag
    10621 ctgctaattc tacctttgca agtgtcacca tacaaacttt taataattat attagcaatg
    10681 ttacaattgc caattccaat ccaacaggat ttctactcaa tacagcaaac gcgaaggaaa
    10741 atttaattta caatatttct atttttaata gccaagatgt tactttcggt gttgggttgc
    10801 gtttagatac aaacgtaaca aaaaacttta tttccggtgt tcttgttgca aataacgcat
    10861 cagatggagt aagtattgta aattctagtg ttaacaatta tatttttaat atatcgtccg
    10921 ttaataacaa tggaattggt gaaaaactaa atagctctaa tacacttcta acaaacgtta
    10981 tgaatctaaa taatggaaat gatcatttgc aaatgaatgg agcgcctacc tctaccttac
    11041 aaaattatgt ttctggagct acacctaata gtcatgtaaa tctaagtggt ggaaatacgg
    11101 atacctattt cacgggtata tttaaagtaa attcagtggc ttgtattact gcaggaggat
    11161 cgtcaggcgg aattactgga acttgtaatg gagctgtaac tggaacaaga ccttcggata
    11221 tcaattatcc atctactaat agttttctaa atttttctgc aactccgctg aaccaaatct
    11281 ttattggaca agcttctagt gagtcggcaa atagctatta cgcatctggc tcaaacacct
    11341 atagcacaag cgttagctgg acaagttttc agaattttta ccgaaaccta ggaaaatccg
    11401 gaacatttgc agcttcctct tcgagggggg catgtattgc gggtggagtt tgtcaaatct
    11461 atgattggac attcaaaact acagatacaa ttggattaaa cactttgcct tgccctggaa
    11521 atactgctca gttgcctgca acagttgtaa ctcatccgtt aaacggaggc ggatctgtta
    11581 cctttcttag aaatgctgtt gaaatcatcg gggatggaat tggagatgac aatggttttt
    11641 gtcaatcctt tgaagcctgt ttatatactc ctaacgtcgg gtcttaccaa ggtcatggaa
    11701 atttagtatc tgcgtccgta gcggttgcaa atacatctac ttgttctgat ataggctcaa
    11761 caggtccagt gacaaacatt actttgtata aatatgatac aaacggatac taacgtttta
    11821 tgagaaatga caggaagaaa agtgagttgg agacaatttt ttactaggtg gaatttcttt
    11881 tttacagatg tcttgggcta taggacaacg agtatgaaag taacagccag atggtttgtt
    11941 tagaacgcct ggaatttcac cacttaagat gattctcttt ttcttcctgt ttgaaagttc
    12001 aaaagtggaa gcaaaaagag attttgtata aggatgcgat ggtttttcaa taatttcctt
    12061 tttacttccc atttctacaa tttttccaag atacatgatt gcaatgtcat ctgagatatg
    12121 tgtaataacg tttagatcat gtgagataaa aagcagagat aggtttttat cttttttgta
    12181 atcagaaagt aaattaatta cttgtgcttg attcgaaaca tcgagtgcag atactatttc
    12241 atcgcaaact ataaattcag gatttaaaat taaagctcgg gcaattgcaa ttctttgtct
    12301 ttgaccccca gaaaactcat gcggataacg atttagaatg tcaggtttta aactcatttt
    12361 ttctaacatc tgaatactta gctcaagtgc ctctttcttt tctgcttttt tatgaacaaa
    12421 taaaccttct gttataattt caaatatagt aagacgaggg tttagtgaag agtaagggtc
    12481 ttgaaagata atttgaagat cttttctcag tgggagcaac tgcctttgac tgagaagagt
    12541 aagatccttg tctttataaa gaatatttcc actttcaaat ggctgtaatc taagaatggt
    12601 tctgcctaac gaagatttgc cgcaaccgga ttctcctact aatcctaaag tatgattctt
    12661 agggattgat aaagacacat cttccactgc tttaatgata ttctttttaa agaattcttt
    12721 tgaatttact ttgtaactgg aatataaatt ctttacttct atcatttctt tttttccatt
    12781 aaaaagcagg cggattgtct accttttttt gattcaaata aaattggttt ctctttttca
    12841 catc
//
LOCUS       DAOQOS010000140        12580 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_2571_length_12580_cov_4.043992, whole genome shotgun sequence.
ACCESSION   DAOQOS010000140 DAOQOS010000000
VERSION     DAOQOS010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 12580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12580
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2571_length_12580_cov_4.043992"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1724
                     /locus_tag="PLF66_21805"
     CDS             <1..1724
                     /locus_tag="PLF66_21805"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HMZ66738.1"
                     /translation="IIIWDQTGEVKYTNQKFLELIAYEESPKSIKVLAKEESNQKESE
                     ILGILHLSPKKYKTYTKEFLKKSREVVLLDVMTSLVRIEDEIYYYSQVRLHKNKSNLS
                     KAETLKKQNEVLLKLTKSETIDSGDLVASVREITEAATSALACQRSSVWFYTEENKSI
                     LSMDLFQLEDGTHSEGAELFAKDFPAYFKYLSEERVLGADNAHTDPSTFEFSEVYLKP
                     LNIQSMLDAPIRLHGKMVGVICNETVGNFRKWTDEELNFSASLADLISRAIEAQIRKK
                     AEDEIKLINENLEHKVEEQTKEIRESLNQITKLKEFQDGDYFLTSLILNPLIGNKNKS
                     EFILTDFIIKQKKQFLFRKSSGQIGGDYCITDNLIFQDEKNRYVFFLNADAMGKSMQG
                     ASGAIVIGTAILNILWQAKNSGIVNVSASEWLINTALELNNIYLTFEGSMILSACFGL
                     IGEANRDLLFLNFEHPHVILLRDGKANFLEINNPNPKLGLLQLNKIPLESKFLHSNDT
                     LIIGSDGKDDIELSSNQESRKINEDEKMILGLVEKSNGNIVELAKLVEEKGVLIDDLS
                     LMSIHLV"
     gene            1974..2369
                     /locus_tag="PLF66_21810"
     CDS             1974..2369
                     /locus_tag="PLF66_21810"
                     /inference="COORDINATES: protein motif:HMM:NF025887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4440 domain-containing protein"
                     /protein_id="HMZ66739.1"
                     /translation="MIDDLIRNEIAKADKAINEKDYDTLKSCYTEDAILVVKPGKLVR
                     GKEEIIIAHKKIAEYFKQTLKVTQGKMLFLEAGDTVLVLSQTFIESPGKIDSEYSTER
                     RATYIYRKVGEHWLCAIDNSYGTELLNSD"
     gene            complement(2383..2970)
                     /locus_tag="PLF66_21815"
     CDS             complement(2383..2970)
                     /locus_tag="PLF66_21815"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HMZ66740.1"
                     /translation="MEPLDKKEIPPASKIFAAAMFNDPLHIWFLPDPTTRLQKLEHLY
                     HFKLKSQWKSAFRVSQNMEGICIWEKPEEQHAAITFSDLYYGLSLLFQVGLVSLWKMI
                     RYQLWSTRLRIKVTNERHWYLNVIVVSPDHQGKGMASKMIKPFLEKASQVGEMAYLET
                     QNVNNVPIYEKYGFVLMESKEIFHTGIFHHCMLKK"
     gene            3381..3806
                     /locus_tag="PLF66_21820"
     CDS             3381..3806
                     /locus_tag="PLF66_21820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66741.1"
                     /translation="MQEIEELNHSILDSLAAQVAVLDSEGNIIAVNAAWRNFCSENSQ
                     EGQRASAELGENYLKICESATGADSEEAKDMYLGLSSVLRQESLNYCIEYPCHSPTEK
                     RWFRAYATKMPFMNSHYFIVAHLNITEEVLNGQITNVRV"
     gene            4249..4863
                     /locus_tag="PLF66_21825"
     CDS             4249..4863
                     /locus_tag="PLF66_21825"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HMZ66742.1"
                     /translation="MELNEIKDRMHNQKLYFCNDDSLVKEQLQCLELLYEFNQTRPTE
                     QQKRQSLLKRMFAELGENCYIEPPVHSNWGGKHVHFGNNVYANFNLTLVDDTHIYVGD
                     NVLFGPNVTIASATHPIHPKLRSKQAQYNLPIYIENNVWIGANSVILPGVRIGENSVI
                     GAGSIVTKNIPSNVVAVGSPCRVVRDIQEKDFNFYYKDLKIDLE"
     gene            4949..5116
                     /locus_tag="PLF66_21830"
     CDS             4949..5116
                     /locus_tag="PLF66_21830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66743.1"
                     /translation="MELAIYSKKKNVSFSVIENKIWYAEKFLILNRSTVSPLDKLGDI
                     VSSNEWWKDIF"
     gene            5290..5721
                     /locus_tag="PLF66_21835"
     CDS             5290..5721
                     /locus_tag="PLF66_21835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66744.1"
                     /translation="MIYKKLLIPVAIYFALSLPIFSAPIFYSFGGEKDIKVMDFPDTA
                     NFNSQEGHFDLGYRYKQIKVFFIPVWNYSGVWCGYLNKEDIYLQVEEPELRKLSQAAN
                     LKFPDSASIPIWDAWGGKIIILLLIAVYLFWKYNPIFNKSE"
     gene            complement(6023..6580)
                     /locus_tag="PLF66_21840"
     CDS             complement(6023..6580)
                     /locus_tag="PLF66_21840"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HMZ66745.1"
                     /translation="MKINKNEEINKIQRGDISPIFLYNTPWKKSIPFSSPFAKPVVLF
                     FLRYIGCPICQLEISKIKKNQELLSKYNVFVVLQSKPETVANQISETDLPFAILCDPK
                     EELFKLFKVQNGSLLSYLHPHNLLTLLIALSKGIMHGKFEGKEKQVPAVFVLLPNGKV
                     QYAYYGKRIDDLPNLKVIVSLDLEN"
     gene            complement(6567..7271)
                     /locus_tag="PLF66_21845"
     CDS             complement(6567..7271)
                     /locus_tag="PLF66_21845"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HMZ66746.1"
                     /translation="MEIFLEETELLNFSNPNIQNLIQNRGWNHKTDKEKILEIYNFVR
                     DEILFGYNTEDEKTASEILEEGIGQCNTKGILFMALLRGVSIPCRFHGFTILKNLQKG
                     AISGIYFHLAPKNIIHSWVEVYYKNKWLNLEGFIVDKVYLKSLQKKFKDCKTDFCGYG
                     VATKNFSSPEIEWKEGDTYIQKEGINQDLGIFNDPDSFFKIYKQNLGIAKKFLFQNFI
                     RNQMNQNVNRVRYENK"
     gene            7509..8276
                     /locus_tag="PLF66_21850"
     CDS             7509..8276
                     /locus_tag="PLF66_21850"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HMZ66747.1"
                     /translation="MKIKKESKLFITNGLTLYIGSIIDTIPHKHNAMQITFPVEGDFT
                     IEIEHKMIQTKSMIIRSKCSHRLVGKNGIQMFIFVEPESVYSDALKAFLGDMSYKEIP
                     IDQSLIETLESEIMNHKSITKVLERILSSLQLQIDIESSMDERIKRVLEKIETIHEKK
                     ILIQNLANHVSLSESRLQHLFKSQIGISIKKYLQWKRLVDGVHIILEGKDFTFAAYEA
                     GFSDSAHMSRVFKEMFGINLIDIFHTSQSIQVYICKN"
     gene            8536..9822
                     /locus_tag="PLF66_21855"
     CDS             8536..9822
                     /locus_tag="PLF66_21855"
                     /inference="COORDINATES: protein motif:HMM:NF017183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative Ig domain-containing protein"
                     /protein_id="HMZ66748.1"
                     /translation="MIYKLLILSLCFGCLDAKRVVFDVSSPSGLIVNAAIAALTKITF
                     PVVTPSPSLPLPTLNYSSNSYSFIRYDTISITPTTTGTITSCTIIPTIPAGLSINNTT
                     CAISGSPTGTQSSISYMVNATNASGSTSVSLSIAITTAIIYGGKGTGGVIISSDGGNS
                     YLTYTTANGLGSNSVNWIYVSGSNVYVATAGGVSISTNGGTSYTNYTTANGLGNNNVT
                     GVFASVSNVYAATDGGLSISTNGGTSYTNYTTANGLGSNLTYSVYASGSNVYVVTAVG
                     VSISNNGGTSYSSYTTANGLGNNTVLGIYAVGSTVYVGTASGVSISTNGGTSYTNYTS
                     GLASSYIAGIYVIGSNVYVATLSGVGISADGGQTYTTYNTANSGLLSNMVYYVYVIGN
                     NVYAANGGGISVSINKGLNYTTNFNAAGAVLGVMAQ"
     gene            complement(10044..11318)
                     /locus_tag="PLF66_21860"
     CDS             complement(10044..11318)
                     /locus_tag="PLF66_21860"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HMZ66749.1"
                     /translation="MKTFFIPPEFNIAVAWISDALNPFTLKTTQNIQEVVIKPEDKER
                     ILKLRNDRLLFFSNHPSQAEPMIAYYIANVMATRFHYMATRRAFDFMNGMVGKWFQGT
                     GTFSVMPGVADKESMKMTRYVLSQPSGKLVIFPEGEPMCSENDNLMPFQSGIIKLGFS
                     ALADARETEPDADITILPAFIKYVITTPKEQVAKNIEGHISKIESKLGANAGGRNLLR
                     RFLMVGRILQEKAEKEYGIEVTNEDYNFRIGKIRHTILDRLAQKMKLKHYDQNADAIQ
                     KLRLITTTLELIEVNYPSPDLPKLSQKELDYINNECIKAYDFIVMKPDYLISYPTPER
                     FYEWLQRFESLVLGATPRMLGGEPHHEPRKAHVFFGKTFHFGDYSESYKKNKAETVTK
                     FLTDLRKEMQSMLDESQKLTSPIVEPGDIGGT"
     gene            complement(11809..>12580)
                     /locus_tag="PLF66_21865"
     CDS             complement(11809..>12580)
                     /locus_tag="PLF66_21865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66750.1"
                     /translation="FLGMQLEDTVLKKTKWKGSFHIDFRRMLIVSNHCSSYYDHETNG
                     KYFIPLFLLQGELGAIGLLTGDKNYELYFFGQEIKSFRYKDGVSKTGFSLEEFLGESQ
                     KKSSSPKNRDKKPKTQNQKKISKQSLDRYLENFERVMGFQIPKKIYQFFHKDLNQILG
                     KNMARFLTGYNYNSRAIAELNLKKMNLLIESMNDGYFGSDKFLPLVSIRDQTACICLN
                     LKNKNPVVYFFDFQDGIFKQKYTLDQLFTHLTKDQPNV"
BASE COUNT         4341 a         1935 c         2130 g         4174 t
ORIGIN      
        1 gaattattat ctgggatcaa actggggaag tgaaatatac gaatcaaaaa tttttagaac
       61 tcattgccta tgaagaatct cctaagagta tcaaggtatt agcaaaagaa gaatcaaacc
      121 aaaaagaatc tgaaatttta ggtatattac atctttcccc aaaaaaatat aaaacgtata
      181 ctaaagaatt tttgaaaaaa tctagagaag tagttttact agacgttatg actagtttag
      241 tcagaataga agatgaaatt tactattatt cgcaagttcg attgcataaa aataaatcta
      301 atctttctaa agcagaaacc ttaaaaaaac aaaacgaagt tttactcaaa ttaacaaaga
      361 gtgaaacaat agacagtgga gatttagtcg cctccgtgag agagattacg gaagctgcaa
      421 caagtgcact tgcttgccaa agatcaagtg tttggtttta tacagaggaa aataaatcta
      481 tactaagtat ggatttgttt caattagaag atgggactca ttcagaggga gctgaacttt
      541 ttgcgaaaga ttttcctgcc tattttaaat atctttctga agaaagagta ttaggggcgg
      601 ataacgcaca tacagatcca agcacctttg aattcagtga ggtttattta aaacctttga
      661 acatccaatc gatgttagat gctccgatcc gattgcatgg aaaaatggtt ggagtgattt
      721 gcaatgaaac cgtgggaaat tttagaaaat ggacagatga agaattaaat ttttccgcat
      781 cgttagcaga cctaatctct agagcaattg aagcacaaat tcgaaaaaaa gcagaagatg
      841 aaattaaatt gatcaatgag aatctcgaac ataaagtaga agagcaaacc aaagaaatta
      901 gagaatcctt aaatcaaatt acaaaattaa aagagtttca agatggagat tattttttga
      961 catcattgat tttaaatcct ttgattggaa ataaaaataa atccgaattc attctaacag
     1021 attttattat aaagcaaaag aaacaatttc tatttagaaa atcatctgga caaatcggtg
     1081 gcgactattg tattacagat aatcttattt ttcaggatga aaagaatcgg tatgtatttt
     1141 ttttaaatgc tgatgcaatg ggtaaatcaa tgcaaggagc aagtggtgca atcgttatcg
     1201 gaactgcgat tttaaacatt ctttggcagg caaaaaatag tggtattgta aatgtatccg
     1261 catctgaatg gctaatcaat actgctttag aattaaataa tatatactta acttttgaag
     1321 gttcgatgat tttatctgct tgctttggtc tgataggaga ggctaatcgt gatttgcttt
     1381 ttcttaattt tgaacatcca catgtcattt tactaagaga tggcaaagca aattttttag
     1441 agataaataa tccaaatcca aagttaggtt tacttcaatt aaacaagatt cctttagaat
     1501 caaaattttt acactcgaat gatacattga tcattggaag tgatggcaag gatgatatcg
     1561 aattgagttc caatcaggag agccgtaaaa tcaatgagga cgaaaaaatg attcttggtc
     1621 tagtcgaaaa gagcaacggt aacattgttg aattagcaaa actagtagaa gaaaaaggag
     1681 ttttgataga cgatctttct cttatgagta tacatttagt atagaaggtg agactctaga
     1741 aggattgatt ggtttcaatt ctacttttta ttttatagag tttctatctc gatctttaaa
     1801 ttcacaatcg aatcattgat ggaaaaagca gtgtgaatta attccatttt taatattctt
     1861 ttgtgttaaa tgaactttct ttgatttgtt aaaaaatata tttcaatgaa atgcagaaag
     1921 gaattgcaaa aaattttacg attcaaataa aaagaataag atgaggagga tatatgattg
     1981 atgacttgat tcgaaatgaa attgcaaagg cagacaaagc gatcaatgaa aaggattatg
     2041 atactttaaa atcttgttat acagaagatg cgatcctagt tgttaagcca ggaaagcttg
     2101 ttcgtggaaa agaggaaata atcattgcac ataagaaaat cgctgaatat tttaaacaaa
     2161 ctcttaaagt gacacaggga aaaatgttat ttttagaagc aggggatacg gttcttgttc
     2221 tatcccaaac atttatcgaa tcacctggta aaattgattc agaatattct acagaaagga
     2281 gagcgaccta tatttatcga aaggtaggtg aacactggtt atgcgctatt gataattcgt
     2341 atggcaccga acttttgaat tctgattaga gaatccttta ttctattttt tcaacataca
     2401 atgatgaaag atcccagtat gaaaaatttc tttgctttcc attaaaacaa atccatattt
     2461 ttcatatatt gggacgttat tcacattttg ggtttctaaa taagccattt ccccgacttg
     2521 agaggctttt tccaagaagg gctttatcat tttacttgcc attcccttgc cctgatgatc
     2581 gggagataca acaatcacat tcaaatacca atgcctttca tttgtaacct tgattctcaa
     2641 acgtgttgac caaagttgat atcgtatcat cttccaaagg ctaaccaaac caacctgaaa
     2701 gaggagagat agaccatagt aaagatcaga gaatgtaatg gcagcgtgtt gttcttctgg
     2761 tttttcccag atacaaattc cttccatatt ttgggatact ctaaaggcac ttttccattg
     2821 cgattttaat ttgaaatgat acagatgttc taacttttgt aaacgagtcg taggatcagg
     2881 taaaaaccaa atatgtaaag gatcgttaaa catagcagct gcaaatattt tggaggcggg
     2941 gggtatttct tttttatcta agggttccat ttttcttcct atctacatac ttttgactca
     3001 atttagagga caaagtgaat gaaatactat tctattctga tacatctttc ttgccttgaa
     3061 ttatttttga atgggagatt attgttattt caaatccttc attgcatttt cccatatttc
     3121 tctttccttt ttagaataca gtcaaaagaa ggttcccttt ttagaagagg ataataaata
     3181 ttatcctctt ctatgaataa aaaaatctct atcgataggt ggagaataaa gattcacaga
     3241 atttgattga attcaaaata gtcgatagtc gatagaaaga aaactacagt attattttta
     3301 aattgtctat cgctttttat tgctttactt ttaaactatg atatcatggt cttcagtaag
     3361 tatagaaaaa ggagaactcg atgcaagaga tagaagaatt aaatcactcc attctagatt
     3421 ctttagctgc gcaagtagcg gttttggact ctgaaggcaa tattattgct gtaaatgcag
     3481 cttggcgtaa cttttgttct gaaaattccc aagagggaca gagagcaagc gcagagttag
     3541 gcgaaaatta tctgaagatt tgtgagtcag ctactggtgc tgattcagag gaagcgaaag
     3601 atatgtatct gggactatcg tctgttctaa ggcaagaaag tctaaattat tgcattgaat
     3661 atccttgtca ttccccaaca gaaaagcgat ggtttagagc ttacgcaaca aagatgccat
     3721 ttatgaactc ccattatttt attgttgctc atttgaatat taccgaagaa gttctaaatg
     3781 gacaaataac aaatgttaga gtatgagttt aacaggtgtt atatgacaaa aacggattct
     3841 aaaaatgtca gttattattt cggacaacgt aataagagta gaagaaatga ttctatttgc
     3901 caaatggata gtgtcgctat agtcagatct gacacaaaaa aacacaatat ttggagaaaa
     3961 gtaacttaga aataaactat ggctaggcta aatgcattgc ttagctcacc caaactagct
     4021 catcaattta tattttagaa aaaaggaata aagaggcgat ggataggcaa caacgtggag
     4081 atacccatta ggcgcttgat ttagtttata gaaagtaaga taaaattttc ctttattcta
     4141 cttattaatt ctatcttgga tttaatttct gttcttagag aaaagtataa aatagtcgat
     4201 tcattaatca atggatgaaa tgatattaaa ctaaaaggaa atcggtttat ggaattaaat
     4261 gaaattaagg atcggatgca caatcaaaaa ttatattttt gtaatgatga tagtttagta
     4321 aaagaacaac ttcaatgttt agaactatta tatgaattta atcagactcg accaacagaa
     4381 caacaaaaac gccaaagctt actaaaaaga atgtttgcag aacttggaga gaattgttat
     4441 attgaaccac ctgttcattc gaactgggga ggcaaacatg ttcactttgg aaataatgta
     4501 tacgccaatt ttaatctgac tctagtagat gatacccata tctatgtagg ggataacgta
     4561 ttatttggac caaatgtaac gatcgcttct gcaacccatc ctatccatcc aaaacttaga
     4621 agtaaacaag ctcaatataa tttaccaatc tatattgaaa ataacgtatg gattggagca
     4681 aattctgtca tattgcctgg agttaggatt ggagaaaata gtgtaattgg tgcaggcagt
     4741 attgtcacaa agaatattcc ttccaatgtt gtcgcagttg gttctccttg tagagtagta
     4801 agagatattc aagaaaaaga ttttaatttt tattataaag atttaaaaat tgatttagaa
     4861 taatccaaaa cggggatgct gtctttggta aggtgggcaa gaaaggtggt aaaatattaa
     4921 acactaacta gttagcaaga gaggctattt ggaattagcc atatattcca aaaaaaagaa
     4981 tgtaagtttt agtgtgatag aaaataagat ttggtatgca gaaaaatttt tgattttaaa
     5041 tcgctcaaca gtctcacccc tagataaatt gggtgatatt gtttctagta acgaatggtg
     5101 gaaagatatt ttctaagttg ttactaaaaa agaaactagt ctcactattt attttgattt
     5161 aggtaggata agtaaaatag agtatgattt tttttattaa cttaaattat ttttaattac
     5221 tctgaaaaaa taaagcggat aattgttatt atcttctgag cgatagaaat tcactcagaa
     5281 ggggaaatta tgatatataa aaaattactg ataccagtag cgatttactt cgcactcagt
     5341 ttaccaatat tttcagcacc aatcttttac agttttggtg gagagaaaga tataaaagtg
     5401 atggattttc cagatacagc aaattttaat tctcaagaag gccattttga tttaggatac
     5461 cgttataaac aaataaaggt attctttatt ccagtctgga attattcagg cgtatggtgt
     5521 ggctatttaa ataaggaaga tatctattta caagtggaag aacctgaatt aagaaaatta
     5581 tctcaagctg ctaatttaaa atttccggac tcagcttcta tcccaatctg ggatgcttgg
     5641 ggaggaaaaa taatcattct acttttaatt gcagtatact tattctggaa atacaatcct
     5701 attttcaata aaagcgaata agtaaaacat ttggattcca tttaacagat gaatcaacta
     5761 tgagaaatag ttccaaatta tatttggttg gaaaatatta tattagccac aactagaata
     5821 tagaatagtg gtaaaattgc tactctaagc aaagctttgt cactattgtt aaaatacatt
     5881 tgaaaagagt caataggcga tatagatata agacaaatta taattataaa tgccaaccat
     5941 agatagaaaa gctatattta caaaaatcta agaatagaat cattgccttt caaagctttt
     6001 ttactttgaa agattgtaga ctctaatttt ctaaatctag cgaaacaatc acttttaaat
     6061 taggcagatc atcaattctt tttccgtagt atgcatattg aacttttcca tttggaagta
     6121 aaacaaaaac agcaggaact tgcttttcct ttccctcgaa tttgccatgc ataattcctt
     6181 ttgataaagc aatcaaaaga gttagtaaat tgtgaggatg cagataactt agaaggcttc
     6241 cattttggac tttaaataat ttaaataact cttcttttgg gtcgcataag attgcaaatg
     6301 gaagatctgt ttctgaaatt tgattcgcaa ctgtttcggg tttactttga agaacaacaa
     6361 aaacattgta tttagagaga agttcttggt tcttttttat ttttgaaatt tccagttggc
     6421 aaataggaca tccaatgtaa cgcaaaaaaa acaagactac tggtttcgca aatggacttg
     6481 aaaatggaat cgattttttc cagggagtat tgtagagaaa gataggagaa atatctcccc
     6541 tttgtatttt attaatttct tcatttttat ttattttcat atcgcactct gtttacattt
     6601 tgattcattt gatttctaat aaaattttgg aaaagaaatt ttttagcaat acctagattt
     6661 tgtttataaa ttttaaaaaa ggaatctgga tcattgaata ttcctaagtc ttgattaatt
     6721 ccttcctttt gaatatatgt atctccctct ttccattcta tttcaggaga agagaaattt
     6781 ttagttgcca caccatagcc gcaaaaatca gttttgcagt ctttgaattt cttttgaaga
     6841 gatttcaaat atactttgtc tacaataaat ccttctaggt tgagccattt atttttataa
     6901 taaacctcta cccaactatg aattatattt ttaggagcta aatgaaaata aattcctgag
     6961 atagcaccct tttgtagatt ttttaaaata gtaaatccat gaaagcgaca gggaatagat
     7021 actcctctta gcaatgccat aaataagatt ccttttgtat tgcattgtcc gattccttct
     7081 tctaaaattt cggatgcagt cttttcatct tcggtgttat atccaaataa aatttcatct
     7141 ctaacgaaat tgtaaatttc tagtattttt tccttatctg ttttatgatt ccaacctcta
     7201 ttctgaatta gattctgaat attcggattc gaaaaattga gtagctcagt ttcttctaaa
     7261 aaaatttcca tatttttcct cgtttcattt atagaaagaa tatcatattt ttgaaataga
     7321 gaattgaacg aatctgctat ggacaaaaaa ataaaaatat ttagaaaact cagaatataa
     7381 aaagatcaca caaaattctt taccttatta ataaagatag aattattttt acaataaatt
     7441 cgatattgaa aaaagtatta tttagctagg atttaatcct atctacttaa atataaaaaa
     7501 ttaaaatcat gaaaattaaa aaagaatcaa aattatttat tacaaatggg ctaacacttt
     7561 acataggctc gatcattgat acaattccgc ataaacacaa tgcgatgcaa attacctttc
     7621 cagtagaagg tgattttaca atagaaattg agcacaaaat gattcagaca aaatcgatga
     7681 tcattcgttc taaatgcagt catcgattgg taggaaaaaa tggaattcag atgtttatct
     7741 ttgtagagcc tgaatctgtt tacagtgatg ctttgaaagc atttttggga gatatgtctt
     7801 acaaggaaat tcccatcgat caaagtttaa tagagaccct agagtcagag ataatgaatc
     7861 acaagagtat tacaaaagtt ttagaaagaa tcctttcttc tcttcaactc caaatagata
     7921 ttgagtctag tatggatgaa agaattaaaa gggttttaga aaaaatcgaa acaatccatg
     7981 agaaaaaaat tctaattcaa aatttagcaa atcatgtttc tttatcagag agtagactac
     8041 aacatttatt taaatctcaa attggtatat cgattaaaaa atatttacaa tggaagaggt
     8101 tagtagatgg agttcatatc attctagaag gtaaagattt tacttttgct gcttatgagg
     8161 caggcttttc agattcggct catatgagtc gagtgtttaa agaaatgttt ggaatcaatt
     8221 taattgatat ttttcatacg agccaatcga tccaagttta tatctgtaaa aattaaaatg
     8281 aaaattctct agataaattg aagcaaatca agagtatgcg atggaattga ttcgaaagaa
     8341 aatattatac attttgttat aaaaaaaata aaattaaaaa tagtttttta tcaaacaaaa
     8401 tgcttgctat agaaaattaa aaaatagact acacaaaagt tttgattcta aaggcgagat
     8461 attttgaata ttgataaaat tgtacagggt aatatagaaa taagattcag gaaaataaga
     8521 ttaaaagtaa aaaatttgat ttataaactt ctgatactaa gtctatgctt tggatgctta
     8581 gatgctaaaa gagtcgtatt cgatgtatca tccccctcag gtcttatcgt aaatgcagca
     8641 atcgcagcac ttacaaaaat tacttttccg gttgtcactc cttcgccttc tttgccgctt
     8701 ccaactttga actatagcag taattcatat tctttcattc ggtatgatac catttccatt
     8761 actcctacca cgacaggaac tattacttct tgcactataa tacctaccat tccagccggt
     8821 ttgagtataa acaatacaac ctgcgcaatt tcaggctctc caactggaac acaatcctct
     8881 atttcttata tggtcaatgc tactaacgca tcaggaagta catccgtaag tctaagcata
     8941 gccatcacta cagccattat ttatggagga aaggggactg ggggagtaat catttcatcg
     9001 gatggtggta atagttattt aacctataca accgccaatg gcttaggaag taattcggta
     9061 aattggatct atgtttccgg ttccaatgtt tatgtagcaa cggcaggtgg agtatctatt
     9121 tctactaacg gagggacaag ctacacgaac tacacgactg caaatggtct aggaaataat
     9181 aatgtcactg gagtttttgc ctcagtatcc aatgtatatg cagctacaga tggaggctta
     9241 tctatttcca caaatggagg aacaagttac acaaactaca cgactgcaaa tggtctagga
     9301 agtaatttaa cctattcagt ctatgcatct ggatctaacg tctatgttgt aacagctgta
     9361 ggagtatcta tctcaaacaa tggtggaacg agttattcta gctatacgac tgcaaatggt
     9421 ctcggaaata atacagttct tggaatttat gccgtcggat caacagtcta tgtaggaact
     9481 gcttctggag tttccatttc aaccaatgga ggaactagtt atacaaatta tacgagtggg
     9541 ttggcaagca gttatatagc aggtatatat gtcattggat ccaatgttta tgttgctact
     9601 ctttcaggag ttggtatttc agctgacggt ggacaaacct atacaaccta taatactgca
     9661 aacagtgggc tattaagtaa catggtatat tatgtttatg ttattggaaa taacgtctat
     9721 gcagcaaatg gaggagggat ttctgtttct attaataaag ggcttaatta tactacaaac
     9781 tttaatgctg cgggcgctgt cttgggagta atggctcagt aaagaaaaaa gactggacta
     9841 caagcaatct atctccagac tccaagtaga accattttct cattttcttt tgtaggctat
     9901 ctaaactaat cattcaatgc ttgtcttttg taaattaaag ttagactagg gacttcatat
     9961 cacttaacgt ctattctatc aatttacaaa atgaccggag ctttttaaat tcaaacctta
    10021 gcaaaaccaa atctctcaga gtattaagtt cccccaatat cacctggctc tacaatggga
    10081 gaggtcaact tttgagattc atccaacata gattgcattt cttttctaag atcagttaaa
    10141 aattttgtaa ctgtttctgc tttattcttt ttatacgatt cagaataatc tccaaaatga
    10201 aatgtctttc cgaaaaacac atgagctttt cttggttcat ggtgcggttc tcctcctagc
    10261 atacgaggtg tagcacctaa aaccaacgat tcaaatcttt ggagccattc atagaacctt
    10321 tcaggagttg ggtagctaat taaataatct ggcttcatca caataaaatc atatgcttta
    10381 atacattcat tgtttatgta atccaattct ttctgactta gttttggtaa atcaggagaa
    10441 gggtaattca cttcaattaa ttctaaggta gtagtaatca gtcttaattt ttgaattgcg
    10501 tctgcatttt gatcgtagtg ttttaatttc attttttgag ctaggcgatc caatatcgta
    10561 tgacgtatct tgccaattct aaaattataa tcttcgtttg ttacctcaat tccatattcc
    10621 ttctctgctt tctcctgcag gattcttcct accatcaaaa atcttctgag tagatttctt
    10681 cctcctgcat ttgcgcctaa cttagattca attttggaaa tatgaccctc tatatttttt
    10741 gctacctgtt ctttgggagt tgtaatcaca tatttaataa acgcgggtag tatagtaata
    10801 tctgcatctg gttctgtttc tcttgcatcg gcaagtgcag aaaatccaag ttttataatc
    10861 ccagattgga atggcattaa attatcattt tcgctgcaca taggctcacc ctctggaaat
    10921 ataactaact ttccggaagg ttgggataga acatatcttg tcattttcat ggattcttta
    10981 tcagcgaccc cgggcattac cgaaaaagtt cctgtgcctt gaaaccattt ccctaccatt
    11041 ccattcataa aatcaaaggc tcttctagtt gccatataat ggaaacgggt tgccattaca
    11101 tttgcaatat aataagcaat cattggctcg gcttggcttg gatgattgga gaaaaataaa
    11161 agtctatcat ttcttagttt taatattcgc tctttgtctt caggtttaat cactacttct
    11221 tgaatatttt gtgttgtttt taatgtgaac ggatttaaag catctgaaat ccatgcgacg
    11281 gcaatattaa attcaggggg tataaaaaat gttttcatag tgttttattt ttacattccg
    11341 catcttatgc aaggaaattt tttccatcca ctctattttt cctaaaccga atgctaaaaa
    11401 aacaccctat cttctgatag actagtcttg aacgagtata tctatagtaa aaaaatagat
    11461 aaccaaattt tttttaagaa ttcttaaaca catttaaaac aaaccaagct ctataaaaag
    11521 aaatccaaat aaatgcgata gccgctagaa aacgatttgg tttgaaatta aaatagaaga
    11581 cttaaaatag aatcttggaa gtaaaattgg ggatttgggc gattcggcac agattcattg
    11641 agaaaaggtg ccgaccgagc tctcactccg gtcaaaaaat tctaaatcgt ttgaatagga
    11701 agcggttatc gcttaaatgg aatcgaatta gaattttttg accccgttcg atctctatcg
    11761 ctttttctaa gtcgaatgct atgtttgagt cccaatcaaa taacgcattt aaacgtttgg
    11821 ttgatctttt gtaagatgag taaataactg atctaaagta tatttctgtt taaaaattcc
    11881 atcttgaaaa tcaaagaaat agacaactgg atttttattt ttcagattta aacaaataca
    11941 ggctgtctga tcccgaatcg agacaagggg aagaaattta tctgaaccaa agtatccatc
    12001 attcatagat tcaatgagta gattcatttt ttttagattt agttctgcaa tggctctgga
    12061 attataatta tatccagtta gaaaccgtgc catattttta cccaaaatct gattcaaatc
    12121 tttatgaaag aattgataaa ttttttttgg aatttgaaaa cccatcaccc tttcaaaatt
    12181 ttctaaatat cgatcgagag attgtttgga gatttttttt tgattttgtg ttttgggttt
    12241 cttatctcga tttttagggg aggaagattt tttttgtgat tctcctagaa attcttccaa
    12301 agaaaaacca gtttttgaaa ctccgtcttt ataccggaaa cttttaattt cctgaccaaa
    12361 aaaataaagc tcgtaatttt tatctcctgt aagtaagcca attgcgccga gctcgccttg
    12421 taatagaaac aagggtataa aatactttcc attggtctcg tgatcataat agctactaca
    12481 atgattggaa acaatcaaca ttcgtctaaa atctatatgg aaactacctt tccattttgt
    12541 ttttttcaat acggtatcct ccaattgcat tcccaagaat
//
LOCUS       DAOQOS010000141        12289 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_2641_length_12289_cov_5.473843, whole genome shotgun sequence.
ACCESSION   DAOQOS010000141 DAOQOS010000000
VERSION     DAOQOS010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 12289)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12289)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12289
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2641_length_12289_cov_5.473843"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            224..565
                     /locus_tag="PLF66_21870"
     CDS             224..565
                     /locus_tag="PLF66_21870"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF037100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HMZ66751.1"
                     /translation="MNSLNFLDSKETEALRKFKDTLIGVYSNEIAGFYLFGSKARGDF
                     NKKSDIDILITIKTDDWKLRDKIRRLGYEFDEEIEYRFSILVLPESKFHYLKENKFQF
                     ALNILRDGVQI"
     gene            562..948
                     /locus_tag="PLF66_21875"
     CDS             562..948
                     /locus_tag="PLF66_21875"
                     /inference="COORDINATES: protein motif:HMM:NF017020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HMZ66752.1"
                     /translation="MSSNPEILAEMARAKKALKSAEVLLNQNLLEDCISRSYYAILHS
                     SKAALLSKGISADSHEAIKNLFGMHLVKSGEIEKEYAFIFREEQDERLLADYDVNFVP
                     EQEMVFRRVEDARDFVMRMEKFLGIA"
     gene            1266..4406
                     /locus_tag="PLF66_21880"
     CDS             1266..4406
                     /locus_tag="PLF66_21880"
                     /inference="COORDINATES: protein motif:HMM:NF023335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3427 domain-containing protein"
                     /protein_id="HMZ66753.1"
                     /translation="MLSQGLYEQIIDKLIFSKISNIDKNSFYIKESKIDKVEAIRVLS
                     LHLTQIFKIALSQYSGEDSLEEQIKLSNKIILLLKNELQNEDFEENLISVEGKILSAI
                     FSKIDSRFSDFEKHLKEITPYTRLSQSELFTGNNAGISLESEIKKEILSCDKICFLVS
                     FIKWTGIRIFERELQEFTDRGGELKIITTSYMGATDLKAVEYLSSLKNTQIKVSYNAD
                     NERLHAKAYLFFRNTGFHTGYIGSSNLSRSALTNGLEWNMKITTQEVSHIIDKFQKTF
                     ETYWLDKEFELFIKSEHSEKLRIALKKEKSFDKNNSTSYFDLKPFPFQEEILEKLQTE
                     RLLHHRFKNLIVAATGTGKTIISAFDFKNFISKNPSAKLLFIAHRKEILEQARTAFQG
                     VLKDNNFGELFVDGIEPEKYNCLFVSVQTLNNRIHSLNLSKSFYDFIIIDEVHHIAAN
                     SYRPILNYFTPKILLGLTATPERMDGEDILKDFCDTIAAEIRLPEALNRKLLCPFQYF
                     GITDTIDLSQVQWRNGRYLPSELTNLYTQNDKRVRDILVSLEKYLRDINDVRALCFCV
                     TQEHAEFMATKFTLAGLKADYLVSSRTNAREELRGKFRKKEINYLFVVDIFNEGIDIP
                     EIDTVLFLRPTESLTVFLQQLGRGLRLAEGKECLTVLDFVGNSRPEYDFEGKFRALIG
                     KTNLSTQKEIEDDFPHLPLGCTIILERKAKDFIIQNIKNATNLNKNQLLNKIRNFKHQ
                     TAQPLTLKNFINFYHIPLQYIYKKGNWKRLCMLAGEIEDYPQENETDIYRAISKKWLS
                     CSSTSYFEFILELSKKNFQVKINDFSENQKLMCLMLHYDIWQNAGGFHSLEESVHAIG
                     KNKVLVEEIMEVLEILLNKINFLESEVTLPYPLPLKLHSRYTREQILAAFGFSSFDKK
                     SSNREGVADNKELNTELLFVDLVKSEKDFSPSTMYQDYAISDTVFHWQSQNATSSEKG
                     KGISYINHISLGKKILLFVRECNEDEFGNTMAYVFLGECIYKGHYGSKPMSINWELKS
                     PMPPFLWKDSAKMAIG"
     gene            4601..5245
                     /locus_tag="PLF66_21885"
     CDS             4601..5245
                     /locus_tag="PLF66_21885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66754.1"
                     /translation="MKKEKEFGLFIIYFLILFLQVEILATSGSDAQFFLLGKKKVEPN
                     FLAVKIYAGEGSLESYFAWNGKKNTLETTRCENDFYFVSAEEGNIDQKKIQSIQKKLE
                     SVSKYSESRSKELSFNCNTTSVKKSEFDFKVYSQKCSFTFRDQKPNVFYFLSKSKKWK
                     SEFYQFDKDTLFLVLSYTNTVESGYKDFHFYVSRKIQTKELACEEWKKEYLSDL"
     gene            5364..6170
                     /locus_tag="PLF66_21890"
     CDS             5364..6170
                     /locus_tag="PLF66_21890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002975226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66755.1"
                     /translation="MENYSEKVARFLDFSILIGSVIVILAWAFGMPLFYNPNGPVMSI
                     FTAISLFTIVGLRLATKYFSLWAFTINIALLLIVGGGNLSSLLMLATAPAIHVNKSSF
                     LVMTSVYTSIGFIFFSIYEILLYLRKTPRNVFILDDILIHLALVPGELSLIGHLFNNP
                     TYLSMGIDPRVGISILEMFFMAALAGSTVLSNKNLFLWQFLKGGVGNQIIFLSLFANQ
                     YIAPLLYLVFVGKSSESYGTELFIMLAGVFATLGFLLIQAYSLNKDQVEA"
     gene            complement(6171..9836)
                     /locus_tag="PLF66_21895"
     CDS             complement(6171..9836)
                     /locus_tag="PLF66_21895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66756.1"
                     /translation="MDKKKLISLLSPESKEILSKKFSEAISLFYLVASDVFEEDRLNL
                     LEELIREEGFQKFLYYRRNSSPGRLEGTLKTLLRRCVLEIPAFILGEIAETMASPSYR
                     QMESAFYTIPIDKEGYEVFERSFFAVGEQRVELLSDYISYCSTDRWFLLRRTFKQYPD
                     LFQDIVNKLANYFIASGDIHKTMDITKNLIHPILIRFISIAYEENHSKEFLMEVFSIL
                     QDTKRAEFFLQLLKIFALRYPPLNTRKLTQEREIFKRLLNLWEVLKILQPEKAKDFKG
                     FLTNYGNPKNEVQVFVWEATLSLQTQKIPQVFTILNSPIFTAIQQYYGQDPATQKFLI
                     KNIINAIAEMQFKKFETKLYKVVLRLLLTLKIDRNFVKRRSNFFDWLKKETNSIRIEL
                     EFMRFTFFEYIFISLGQVKNERSMKMTRFCDDDHKFICKYLDEIQLAAKGRIKDISLI
                     YNPILEKIRQILYLPLLGKFPEYEYALTELQSASISIKQEDGLYTALEVVSKEHFINY
                     YETFMEVFKTNPENMYLAILPDYWYRITLEKAKPYIHGVVLPVVGSISIEEGEMGAYT
                     DGKTIFLPGFVNYFPDSIEPIEENRNLTIYIALSLHECGHIIAGSFQFDLAYYISKLE
                     KPSLFQIIMNAMEDYRIERFLIRIKAHSQIEELLHTMNEYFTFMNIRNPENLAMNLVF
                     FIMDEAAGYNTLSKVQSNYQKSIEELFKSNLYSGRFNSIQDMAEYFVVRLKSIDIANP
                     LSAYPVARELYEILKVWPDTALASLASPEYFPTGLHSMEGKNEIERQRPLNQDELDAL
                     YKEYNENPKAFLERNHLPVFPELFGEETEEGKSETLAVTGKAKEYIEELLREKIAQEY
                     SQAGTIDLSHRTKVDDLIADNQRKKTNDTNENKKDIEDTKENKKSSKKKKTKSKTKRI
                     YSIDPKTKSRTRLTEIKEYKVRNVNPLFMKKFAKWKYISQQVLRELSALLPTIQEEHD
                     TSTLEGELNMELLIEILSDRSKLGSVEFLDIYKESSRSVEVIIGLDISGSTDLMIQDI
                     PIPKGTPKMVNGMSVIELNLSRADQIKYDTILDIEKAFAMIFAEALSYITKNVGIYAF
                     NSVTSTNVYKAETIESVSSFVSDSANRDGDFIRYIHDILGKSEAEVKYFYLITDGRPS
                     ADNYSGKDALDDTLIAMREVVNSGIKLIYFNIDSQKQEYFELFQKEATFAQHFQSPED
                     ILPVIPELVKTVVRSIQ"
     gene            complement(9829..11514)
                     /locus_tag="PLF66_21900"
     CDS             complement(9829..11514)
                     /locus_tag="PLF66_21900"
                     /inference="COORDINATES: protein motif:HMM:NF019346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HMZ66757.1"
                     /translation="MNIEKQMQQEIFIKNILTLLSTDKSVKIMEKKSFTNSVRELNIA
                     NDLLSQWTNSWKQSSNKTLYLPGDAKEIQNMLLFLFSALLSDENPVSTAFDTLFAEFP
                     NGNLDRKKGTELIQQRTNLQTEKSFQKRIDLFKDKILLSSASIKNGTVNQKFYIANQV
                     TYEGLDEIQRLLLAYKLGIHVAIDGPPGVGKTQSILEFGNILKKNVYTKTCSSRTTES
                     HIISFPVLTVQEGVSVTAQENGPLVRAMLEGSIFYGDEFNLLKEDVQKRMNSAFDERS
                     SIDRMDGMMVQAQKGFWGAISYNPTNNMVARDLEESVADRFLHLHYDRWGADFKALVA
                     SSKAKGTLPSSKIMELSYGIKLEWRGIGENLKFYRGEEANDGKINWFEFFTDAPAKDT
                     PFYIYRSYDQNSIFQTKTQNLKEDLSNLATNAFSEQELARMISRFTELLISLSESGES
                     PILKKIGLADLKEKEDLELLSLHESSTRIEVAALKHYHELINKGWNRYLAQSYSVRIV
                     IDQICYGQFRNKKLRENTTYTLVNLIAKNMKLFADTSKYNTKMLTDSLLKQNG"
     gene            11634..12188
                     /locus_tag="PLF66_21905"
     CDS             11634..12188
                     /locus_tag="PLF66_21905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66758.1"
                     /translation="MIKIYIGILFIGLIHPLVSIERLEVKEIQYKIENEIVSQEKYSE
                     FLRKLSFVRFTEFYRNNPEKTYFKNYRSGLIFYEEKLILEKTKALFLIRKIQMTGNQI
                     YKINEKYVSKEEFDLYLKDLKEIQETWYCKKESTGGTTGYDAKDQSDNIYKIIQSSYE
                     EIGINVIVEKKNVNFKFPLLIEKN"
BASE COUNT         3926 a         1877 c         2064 g         4422 t
ORIGIN      
        1 tcccccattc cttttacgct tcgctatgaa tgtcccatca tcgctcagtc gcgattctat
       61 cttttcacga acatcgttga cttgtggcta agttggatca agtctcttgg tcatgatact
      121 tttaccgcgt cttgttttct aaatttattc tagagtgatt atttgaattt agcaaaattt
      181 aataatttac aataaataga attaaaaatt gaatagaggc agaatgaatt ctctaaactt
      241 tttagattct aaagaaaccg aggcgttaag aaaatttaag gatactctta ttggagtata
      301 ctcaaatgaa attgctggtt tttatctttt tggttctaag gctagaggag attttaataa
      361 aaaatccgat atagatattc ttatcacaat taaaacagac gattggaaac ttagagataa
      421 aattcgtaga ttgggatatg aatttgatga agagattgaa taccgatttt ctattcttgt
      481 ccttcccgag agtaagtttc attatttaaa agaaaataaa tttcaatttg cgttaaatat
      541 tttgcgtgat ggagttcaaa tttgagttcc aatcctgaaa ttcttgcaga aatggcaagg
      601 gcaaagaaag cattaaagtc tgccgaagtt ttgttaaatc agaatttact cgaagattgc
      661 atttctagaa gttactatgc aattttgcat tcttctaaag cagcgctttt atccaaagga
      721 atttctgctg attctcatga agcaatcaaa aatttgtttg gtatgcactt agtaaagtct
      781 ggtgaaattg aaaaggagta tgcgttcata tttcgagaag aacaggatga aagattgctt
      841 gcggattacg atgtaaattt tgttccagaa caagagatgg tttttcgtcg tgtggaagat
      901 gctcgggatt ttgttatgag aatggagaag tttttgggca ttgcttaata acagttgact
      961 agtttattaa tactctcaaa taatttgatt tttaattcaa tctatttcgt ctgaattcct
     1021 cataaccatg tttgccgtca gtcattagta tgataatatt cctccattta tcaggcttga
     1081 atgtgcttat aaggaaaaag atttcgaact tagttcattt atttttatct caattacaat
     1141 ttccaactca ttcttaaatc gatttgggga actttgtatt tttaaaatag aaggggattt
     1201 tgagttttgt aatagacgtt taaattactt gagagagctt gagtgaaaag gataggattc
     1261 aaaggatgtt atcgcaggga ttatatgaac aaatcattga taaattaatt ttctctaaaa
     1321 taagtaacat tgataaaaat tcattttata tcaaagagag taagattgat aaggtagaag
     1381 ctattcgtgt tttgtctcta cacttaacgc aaatttttaa aatcgcattg agtcaatatt
     1441 cgggtgagga tagtctagaa gaacaaatta aactttctaa taaaatcatt ttgcttttaa
     1501 agaatgaatt gcaaaacgaa gattttgaag aaaatttaat ttcggtagaa ggtaaaatac
     1561 tttctgctat cttttctaaa atcgattcta gattttctga ttttgaaaaa catctaaaag
     1621 aaataacgcc ttacactcgt ttgtcgcaga gtgaattgtt tacagggaat aatgctggta
     1681 tctctctaga aagtgaaatc aaaaaagaaa ttttgtcctg tgataaaatc tgttttcttg
     1741 tgtcgtttat taaatggact gggattcgaa tctttgagag ggaattacaa gagtttaccg
     1801 atagaggagg ggagttaaaa ataatcacca cttcttatat gggtgcaacg gatctaaaag
     1861 cagtcgagta tttatctagc ttaaagaata cgcaaattaa ggtttcttac aatgcagaca
     1921 acgaaagact ccatgcaaaa gcttatttgt tttttagaaa tacaggattt catactggtt
     1981 atattggctc ctctaatttg tccaggtctg ctttaacaaa tggacttgaa tggaatatga
     2041 aaataaccac ccaagaagta agtcatatca tcgataaatt tcaaaaaacc tttgagacct
     2101 attggttaga caaagagttt gaattattta taaaaagtga gcattctgaa aaattaagaa
     2161 tcgcacttaa gaaagaaaag agttttgata aaaataattc aacttcttac tttgatttaa
     2221 aaccctttcc ttttcaggaa gaaattttag aaaaactaca aacagaaaga cttcttcatc
     2281 atcgttttaa aaatttaatc gtagccgcta ctggaactgg aaaaacaatt atctctgctt
     2341 ttgattttaa gaattttatt tcaaaaaatc ctagtgcaaa acttttattt atcgcacatc
     2401 gaaaagaaat tttagaacaa gcaagaacag cattccaagg tgttttaaaa gataataatt
     2461 tcggagaact ttttgtagat ggaattgaac ctgaaaaata taactgttta tttgtatcgg
     2521 ttcagacttt gaataatcga attcatagtt taaatctttc aaagtctttt tatgatttta
     2581 tcatcattga tgaagttcat catattgcgg ctaatagcta tcgtccaata ttaaactatt
     2641 tcacaccgaa aatcctttta ggacttactg ctacacctga gcgtatggat ggagaagata
     2701 tcttaaaaga tttttgtgat acaattgccg ctgaaattag acttccagaa gcactcaatc
     2761 gaaaacttct ttgtcctttt caatattttg gaattacgga tacgattgat ttgtcgcagg
     2821 ttcagtggag aaatgggaga taccttccga gtgaacttac aaatctttat acccaaaacg
     2881 acaaaagagt tcgagacata cttgtaagtt tagagaaata tttgcgagat ataaatgatg
     2941 ttcgtgctct gtgtttttgt gttacacaag aacatgcaga gtttatggca acaaaattta
     3001 ctttggcagg gttaaaagca gattatttgg tgagttctag aactaatgct cgagaggaac
     3061 tgcgaggaaa atttcgaaag aaagaaatta attatctctt cgtcgtcgat atttttaatg
     3121 aaggaattga tattcctgaa atcgacacag ttctattttt aagaccaacg gagagtctaa
     3181 cggtctttct ccaacaactt gggcgagggc ttcgtcttgc agagggtaag gagtgtttga
     3241 ctgtattaga ttttgttgga aattctagac cggagtatga ctttgaagga aagtttagag
     3301 cactcattgg aaagactaat ttatctactc aaaaagagat tgaagacgat tttcctcatc
     3361 tacctttagg ttgtactatt attttagaac gaaaggcaaa agattttatc attcaaaata
     3421 ttaaaaatgc aactaaccta aataaaaacc aactgctgaa taaaattcga aattttaagc
     3481 accaaactgc gcaacctctt actttaaaaa attttatcaa cttctatcac atcccattgc
     3541 agtatattta caaaaaggga aattggaaac gactctgtat gcttgcaggg gagatagagg
     3601 attatccgca agaaaatgag actgatattt acagagctat cagtaaaaaa tggctttctt
     3661 gtagttctac atcttatttt gaatttatac tggaattatc caagaagaat tttcaagtga
     3721 aaataaacga tttttcagaa aatcaaaaac taatgtgttt gatgcttcac tatgatattt
     3781 ggcaaaatgc aggtgggttt cattcgttag aagaaagtgt tcatgcaatt ggaaaaaata
     3841 aagtcctagt agaagaaata atggaagttc tagaaattct tttgaacaaa atcaattttt
     3901 tagaatcaga ggttacgctt ccttatccac tacctttaaa gcttcatagc cgatatacca
     3961 gagaacaaat tttagccgct tttggattta gttcttttga taaaaaatca tctaatcgag
     4021 aaggggttgc tgataacaaa gaattgaata cggaacttct ttttgtcgat ttagttaagt
     4081 cagaaaagga tttttctcct tccacaatgt accaagacta tgcgataagc gatactgtat
     4141 ttcattggca atcgcaaaat gctacaagtt cagagaaagg aaaaggtata tcttatatta
     4201 accacattag tcttggaaaa aaaatcttac tttttgttag agagtgtaac gaagatgagt
     4261 tcggaaatac aatggcttat gtatttttgg gagaatgcat ttacaaagga cattatggtt
     4321 ccaaaccaat gagcataaat tgggaattaa aatctcctat gccacccttt ctctggaaag
     4381 attctgcgaa aatggcgatt ggttaaaaca gactatcaga aatattctat ttttgatagc
     4441 taaaattctt tcaacgagtt gcaagccaac ataagaccta tctattcttg gttttctcta
     4501 cttcaaataa aagcgcacga ttttttcgta ttttttaaag ttgtgtatgg gatgtgaaat
     4561 tatcttgagg tggattggta ttttttttga attgaaagga gtgaaaaaag aaaaggagtt
     4621 tggtttgttt attatctact ttttaatttt atttttgcag gttgaaatat tggctacttc
     4681 tggatcggat gctcagtttt ttcttttagg aaaaaagaaa gttgaaccaa attttttagc
     4741 agtaaaaatt tatgcaggag aaggttctct ggaatcgtat tttgcatgga atggaaaaaa
     4801 gaatacttta gaaacaacac gctgtgaaaa tgatttttat tttgtttctg cagaagaggg
     4861 aaatatagat caaaaaaaaa ttcaatctat tcagaaaaaa ttagaatctg tatctaagta
     4921 ttctgaatct agatcgaaag aactttcttt taattgcaat actacttccg taaaaaaatc
     4981 cgaatttgat tttaaggttt attcgcaaaa atgcagtttt acctttcgag atcaaaagcc
     5041 gaatgtgttt tactttctat ctaaatcaaa gaaatggaag tctgagtttt atcaatttga
     5101 taaagacact ctctttttgg tattatccta tacaaataca gttgaatctg gatataaaga
     5161 ttttcatttc tatgtttcta gaaagattca aacaaaagaa ctagcttgtg aagaatggaa
     5221 gaaagaatat ctttctgatt tatagttttt aataaatctt ttcagaagaa ttgatttctt
     5281 tcttttaaaa aacacgtaac gcattaatta tcctttgaca gttcaaatca gaattctaat
     5341 ttgatccaaa aggaatggtg agtatggaaa attattcgga gaaagtagct cggtttttag
     5401 attttagtat attgattgga tcagtgattg tgattcttgc ttgggcattt ggaatgcctt
     5461 tattttataa tccaaacggt cctgtgatgt cgatttttac agcgatctct ctttttacta
     5521 ttgttggact tcgtcttgct acaaagtatt tttctctttg ggctttcacc atcaatatcg
     5581 ccttgttgct tattgtggga gggggaaatc tttcttctct tttgatgctt gcaactgctc
     5641 cagcgattca tgtaaataaa tcatcctttt tagttatgac ttctgtttac acttctattg
     5701 gttttatttt ttttagtatc tatgaaatac ttctatatct acgcaaaact ccgagaaatg
     5761 tttttattct agatgatata ttaatccatt tagcgttagt tccaggggaa ttaagtttaa
     5821 tcggtcatct atttaacaat ccaacttatc taagcatggg aattgatcct agagttggaa
     5881 tctctatttt agaaatgttt tttatggctg cactcgctgg atctacagtt ctttctaata
     5941 agaatttatt tctctggcaa tttttaaaag gtggagtagg caaccaaatc atatttttat
     6001 ctttatttgc caatcaatac attgcccctc ttttatatct agtatttgtg ggtaaaagtt
     6061 cagaaagtta tggaactgag ctctttataa tgttggctgg agtttttgca acgttaggct
     6121 ttctattaat tcaagcttac agtttaaata aagatcaagt agaggcttaa ttattgaatc
     6181 gaacgcacaa cagtttttac taattctgga atgactggta atatatcttc tggagattga
     6241 aaatgttgtg caaaggttgc ttccttctga aataattcaa agtattcttg tttttgggaa
     6301 tctatattga aataaattaa tttgattccc gagttgacta cttctctcat cgcaatgagt
     6361 gtatcgtcta aagcatcttt tcctgaataa ttatctgcac tcggtctacc atcagtgatt
     6421 aaatagaagt attttacctc tgcctcgctt tttcctaaaa tatcatgaat ataacgaata
     6481 aaatccccat ctcgattcgc tgaatcagac acaaaggaag atactgactc aattgtttct
     6541 gctttataga cgttagtaga tgtaacagaa ttaaaagcat aaattcctac attctttgta
     6601 atatacgaaa gagcttctgc aaatatcatc gcaaatgctt tttctatatc cagtatcgta
     6661 tcatatttta tttgatctgc tcttgataaa tttaactcta tcactgacat tccatttacc
     6721 attttaggag ttccctttgg aatgggaatg tcttgaatca ttaggtcagt agacccgctt
     6781 atatccaaac caataatcac ttcaacagat ctagatgatt ctttgtatat gtctagaaat
     6841 tcaactgatc ccaatttaga tctatcactt agaatttcta taagtagctc catatttaac
     6901 tccccttcta atgtggaagt atcatgctct tcctggattg tgggaaggag tgccgaaagt
     6961 tctctcaata cttgctgtga tatgtatttc cactttgcaa attttttcat aaagagtgga
     7021 tttacatttc ttactttgta ttcttttatt tctgttaatc tagttctaga ctttgtttta
     7081 ggatcaatgg aataaattct tttggtttta gatttagttt ttttcttttt agatgatttc
     7141 ttattttctt ttgtatcttc tatatccttt ttattctcat ttgtatcatt tgttttcttt
     7201 ctttgattgt ctgcaatcaa gtcatctact tttgttctat gagataaatc tatcgttccc
     7261 gcttgtgaat attcttgagc aattttttct cttagtagtt cttctatgta ttcttttgct
     7321 ttgcctgtga ctgcaagtgt ttctgatttc ccttcttctg tttcctcacc aaatagttct
     7381 ggaaaaacag gtagatggtt tctctctaaa aatgcttttg gattttcatt gtattcttta
     7441 tataaagcgt ctaactcatc ctgatttaaa ggtctttgtc tttcgatttc attttttcct
     7501 tccatgctat gaagaccggt tggaaagtat tcaggagaag ctaagcttgc tagtgcagtg
     7561 tcaggccaaa cttttagtat ttcgtataac tctcttgcaa ctggataagc agaaagtgga
     7621 tttgctatat cgatggattt taatcttacc acaaagtatt ctgccatatc ttggatggaa
     7681 ttaaaccgac cagaatatag atttgatttg aataattcct caatggattt ttgataattg
     7741 gattgcactt ttgataaagt gttataaccg gctgcttcat ccatgataaa aaagactagg
     7801 ttcatagcca gattttcagg attacgaata ttcataaaag taaagtattc attcattgta
     7861 tgaagtaatt cttcaatctg ggaatgagct ttaattcgaa ttaaaaatct ttcgatacga
     7921 tagtcttcca ttgcattcat tataatctga aataaacttg gcttttccag ttttgaaata
     7981 taatacgcta aatcaaattg gaaacttccg gctatgatat gtccgcattc atgtaaagag
     8041 agtgcaatat aaatcgtaag atttcgattc tcttcaattg gctcgataga atctggaaag
     8101 taatttacaa atccaggaag aaagatcgtt ttcccatctg tgtaagctcc catctctcct
     8161 tcttcaatgg aaatagaacc gacaaccggc aagaccacgc catgaatgta aggctttgct
     8221 ttttcgagtg taattctata ccaataatct ggaagtattg ctaaatacat attttccgga
     8281 ttggttttga agacttccat gaaggtctca tagtaattta taaaatgctc ttttgaaaca
     8341 acttctagag cggtatacaa tccatcttct tgtttaatac taatagatgc actctgtagt
     8401 tcggttaacg catattcata ctctggaaac tttcctaaaa gcggtagata taaaatctgt
     8461 ctaattttct ctaagattgg attgtagata agagaaatat ctttaattct acctttggct
     8521 gcgagttgaa tctcatctaa atatttacag ataaatttat ggtcatcatc acagaaccga
     8581 gtcatcttca ttgatctttc attttttact tgtccaagtg aaatgaatat atattcgaaa
     8641 aaagtaaatc tcataaattc caattcgatt cgaatcgagt tggtttcttt ttttaaccaa
     8701 tcaaagaaat tacttcttcg ttttacaaaa tttcgatcaa tctttaatgt aagaagcagt
     8761 cttaaaacaa ccttgtagag ctttgtttca aatttcttga attgcatttc ggctattgca
     8821 ttgatgatgt ttttaattaa aaacttttgt gtggcaggat cttgtccata atactgttga
     8881 atcgctgtga agattggtga gttcagaatt gtaaatactt gtggaatctt ttgtgtttga
     8941 agactaagtg ttgcttccca tacaaagact tgcacttcat tttttggatt tccgtaattg
     9001 gttaaaaatc ctttaaagtc ctttgctttt tctggttgta gaatttttaa gacttcccaa
     9061 aggttcagta atcttttaaa gatctctctt tcttgcgtca acttccttgt attgagaggt
     9121 ggatatcgta atgcaaaaat ttttaacagt tgtaagaaaa attctgctct ctttgtgtct
     9181 tgcaaaatgg aaaaaacttc catcaaaaat tcctttgaat gattttcctc ataagcaatc
     9241 gaaataaacc gaatcaaaat aggatgaatt agatttttag tgatgtccat cgttttgtgg
     9301 atgtcacctg acgctataaa atagttcgca agtttattta caatatcttg gaatagatct
     9361 ggatactgtt taaaagttct tctaagtaaa aaccatcgat ccgtagaaca gtaagaaata
     9421 taatcagaga gtaattctac tctttgttcc cccacagcaa aaaaacttct ttcaaatact
     9481 tcgtatcctt ctttatcaat tgggattgtg taaaaagcag attccatttg tctataagaa
     9541 gggcttgcca ttgtttctgc aatttctcct aggataaaag ctggaatttc taagacacaa
     9601 cgcctgagta aagtttttaa agttccttct agtctaccag gcgaggaatt tcttctataa
     9661 taaaggaatt tttgaaatcc ctcttctcga atcaattctt ctaatagatt gagtctatct
     9721 tcctcaaaga catcactcgc tactaaatag aataacgaaa tagcttctga aaatttcttc
     9781 gaaagaattt ctttactttc aggagagagt agactgatta atttcttttt atccattttg
     9841 ttttaagaga gaatcggtta gcatcttagt gttgtatttg gatgtatctg caaaaagctt
     9901 catatttttt gcaatcaggt tgactaacgt ataggttgtg ttttctctca gctttttatt
     9961 tctaaattgc ccatagcaga tttgatcaat tacaattcta actgagtaag attgtgctaa
    10021 ataccgattc caacctttgt ttattaattc atgataatgt tttaatgcag ccacttcgat
    10081 acgagtagaa ctttcgtgta gacttaataa ttccaagtcc tctttttctt ttagatctgc
    10141 taatccgatt ttttttagaa ttggcgattc tccagattct gaaagagaaa tgagtaattc
    10201 tgtaaatcta gaaatcatac gagctaattc ttgttctgaa aatgcatttg tggctagatt
    10261 cgaaagatcc tcttttaaat tttgtgtttt agtttgaaag atactatttt gatcataaga
    10321 acggtaaata taaaaaggtg tgtcttttgc tggtgcatct gtgaagaatt caaaccaatt
    10381 gatttttcca tcgtttgctt cttctccgcg atagaacttt aaattctctc cgatacctct
    10441 ccattctaac ttgattccat agcttagttc cataatctta gaagaaggaa gagttccttt
    10501 tgctttacta ctagctacta acgctttaaa gtcagcaccc catctatcat aatgcaaatg
    10561 tagaaatcga tctgccaccg attcttctaa atctcttgct accatattat tagtagggtt
    10621 ataagaaatt gcaccccaga atcctttttg tgcttgcacc atcattccat ccattctatc
    10681 gatagatgat ctctcatcga atgcggaatt cattcttttc tgcacgtctt cttttaaaag
    10741 attgaattca tctccataaa aaatagatcc ttctagcatg gctctaacta atggtccatt
    10801 ttcttgtgct gtaacactta caccttcttg aacggttaga acaggaaaag atataatatg
    10861 cgattctgtt gttctagatg aacaggtctt tgtataaaca tttttcttta gaatatttcc
    10921 aaattctaaa atactctgag tcttaccaac tcctggaggt ccatcgattg caacatggat
    10981 tccaagttta taggcaagga gtagtctttg tatttcgtct aacccctcat aagttacttg
    11041 atttgcaata tagaattttt gatttacagt tccatttttt atagaagctg atgagagaag
    11101 tattttatct ttaaataaat cgatcctctt ttggaaagat ttttcggttt gtaaattggt
    11161 tctttgttga attaattcgg ttcctttttt gcgatctagg tttccatttg gaaattccgc
    11221 gaagagtgtg tcaaaggcag tggagactgg attctcatcc gagagtagag cagagaaaag
    11281 aaatagcagc atattttgaa tttcttttgc atcgcctgga aggtagaggg ttttattgga
    11341 agattgcttc caagaatttg tccattggga gagaagatca tttgcgatat ttaattccct
    11401 aacgctattt gtgaaacttt tcttctccat gatttttacg gatttatctg tggataatag
    11461 cgttaggata tttttaataa aaatttcttg ctgcatctgt ttctcaatat tcattttctt
    11521 tcccttctct gaaattggtt tgaatttctt ctagattcag attttgtcta ggagaaatta
    11581 acgttttgta tttgttattc ttggtaatta aaagagagat tgtaaaaata gatatgataa
    11641 agatatatat tggaatatta tttataggtc tgatacaccc tttggtttcg atagaaagat
    11701 tagaagtaaa agaaattcaa tataaaatag aaaatgagat tgtaagccaa gagaaatatt
    11761 ctgaattttt aagaaaactt tcttttgttc gctttactga attttataga aataatccag
    11821 agaaaacgta tttcaaaaat tatagaagtg gtcttatttt ctatgaagag aaactcattc
    11881 ttgaaaaaac aaaagctttg tttttgattc gaaagattca aatgacagga aatcaaatct
    11941 ataaaatcaa tgaaaaatat gtttcaaaag aagaatttga tttatatctg aaagatctta
    12001 aagaaattca agagacttgg tattgcaaga aagaaagcac aggcggaaca acaggttatg
    12061 atgctaaaga tcaaagtgat aatatttata aaatcattca aagttcttac gaagaaatag
    12121 gaattaacgt gattgtagaa aaaaagaatg taaattttaa attcccttta ttgatagaaa
    12181 aaaactaacg gactgaatca ttcattcttc ttttgtgatt gagtttatgc tcatacattt
    12241 tcatatccgc acttttgata aaatcatcta tatgaatttt ttcttcttt
//
LOCUS       DAOQOS010000142        12034 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_2708_length_12034_cov_6.465732, whole genome shotgun sequence.
ACCESSION   DAOQOS010000142 DAOQOS010000000
VERSION     DAOQOS010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 12034)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12034)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12034
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2708_length_12034_cov_6.465732"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            113..319
                     /locus_tag="PLF66_21910"
     CDS             113..319
                     /locus_tag="PLF66_21910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66759.1"
                     /translation="MPKLIRNTEVVERCRRLYKDGHSMPQIAEKIPIEFPYIKSIHPN
                     TIFNAIHFGVNTRSKLNVARPTNS"
     gene            294..1073
                     /locus_tag="PLF66_21915"
     CDS             294..1073
                     /locus_tag="PLF66_21915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66760.1"
                     /translation="MLLDLPTAKKALTNVCTNAPADARFQLKIKNKSGFSITIPQLKF
                     PGFLEPGQYEFFAFQSNPEGEFLEVLSAELVIEAMEESTPKAVNYEQPQPVSQQATQQ
                     TNRQYQEFVSPDFILKLLEKDQAVFSNERKLHDEKVSMIMQNHTKEIERIKEAHQKEI
                     TSLKDSHSIEVNNLKTLNEKIDAERDKIRESIEKQLRGERKEQARAGKKSVWDIIADF
                     VEKNPDEILAFIQSKDRGNSQTETRYSNLQEAIKEAREAGL"
     gene            1073..1243
                     /locus_tag="PLF66_21920"
     CDS             1073..1243
                     /locus_tag="PLF66_21920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66761.1"
                     /translation="MSIEELEKRIKKEELKNKVFRSIFKTFYEEIKSQLPKAFVKDVD
                     NAFAQLDSMDKL"
     gene            1250..1873
                     /locus_tag="PLF66_21925"
     CDS             1250..1873
                     /locus_tag="PLF66_21925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66762.1"
                     /translation="MAKKKAIYIENGSFKILQDSDSLMSSGSGIYNFTSSNNRLYSSP
                     SNGTSLSTAALVANTLYAMPFISEKRFIVSLLKMSVTVASAGSTVRAGIYSDNGSCYP
                     NFKLNDLGQASGASVAVIQFSNSLPVSYAENLFWLVCVVSAAITVKGFSVAGLIPLLG
                     TDSNIGNAPGFGYSISTGVSTLPASFPTGAAIRTAAPLPYMGLFITG"
     gene            1887..2351
                     /locus_tag="PLF66_21930"
     CDS             1887..2351
                     /locus_tag="PLF66_21930"
                     /inference="COORDINATES: protein motif:HMM:NF024929.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HMZ66763.1"
                     /translation="MRDIKLCVPTLQEAWKYSQEFWSKNFPGKPQPILTCTLRTIAEQ
                     KALFAQGRLPLSEINKLRKIAGMAAISASEGKKKVTNADAGQSKHNPDKTGLSRAFDV
                     GFLTISSGKNHSLVWDEINFKEFNKIISAKFPLVQWGGNWKSFKDYPHFEVK"
     gene            2351..2785
                     /locus_tag="PLF66_21935"
     CDS             2351..2785
                     /locus_tag="PLF66_21935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016752584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1353 domain-containing protein"
                     /protein_id="HMZ66764.1"
                     /translation="MKYKELKNYKYQLAENYSIQTELKPVNAIFEPNKEKPFIELDTN
                     GVLTIFSGYSWDGASGIAIDTNNFIRGSLVHDAFYQLMRKRKLSLAHRDYADRLLQKI
                     CKEDGMSSFRANNVYYAVKIFGESSAAPTSKKEIQETILEAP"
     gene            2790..3041
                     /locus_tag="PLF66_21940"
     CDS             2790..3041
                     /locus_tag="PLF66_21940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66765.1"
                     /translation="MIVSEDLINGILREIDVMYSEGLLKIDDADDLSKSVGWINGKSL
                     NESNVSYVIECLRGYKKFMNAEAVTRIINLCRKTFGSYM"
     gene            3043..3297
                     /locus_tag="PLF66_21945"
     CDS             3043..3297
                     /locus_tag="PLF66_21945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66766.1"
                     /translation="MESEKKIDLKEFMESFKEHIENEYPNAPKVNIESPLIQPQEKNF
                     RYYFGIFGESLILIGMYAIPLSEVSLAAYGLYVLLRYGII"
     gene            3524..3877
                     /locus_tag="PLF66_21950"
     CDS             3524..3877
                     /locus_tag="PLF66_21950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66767.1"
                     /translation="MAEKEDLSKKGLTFFTRAFDHRTYTADGAFSQSFNDKIILDFYV
                     DRIVYPTKIVFDLDKDDNFIEPGKFSTFAKITTERNIQHSLTINLKTAIELKETLEEA
                     INDLINMEEKENESN"
     gene            3864..4355
                     /locus_tag="PLF66_21955"
     CDS             3864..4355
                     /locus_tag="PLF66_21955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66768.1"
                     /translation="MNLTKNIRPAKANYSFPTNHNLRIGGKGSTQSQTELNFNMIFDF
                     LNFILSFNQEQKEEIVEQYNNLPARQTFKRNTIAIIQSLKGFEVLEPIKIEISIEYEE
                     YLVEIPELEIYAYDKDIHECIKEIKEDIIRLYYDLNRERKEILGEKPKKWKDFLNYSI
                     KKI"
     gene            4352..4678
                     /locus_tag="PLF66_21960"
     CDS             4352..4678
                     /locus_tag="PLF66_21960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66769.1"
                     /translation="MSKRKLFTEKEIREKILTKVDPQNINKDGKHWIGEIILNNVFIN
                     KVKIPNSHKDTMFHNKTKKIAEDLCITNDEFTDLIDCSLSGKDYYKKLDEKFNPKEKT
                     LSEENP"
     gene            complement(4809..4955)
                     /locus_tag="PLF66_21965"
     CDS             complement(4809..4955)
                     /locus_tag="PLF66_21965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66770.1"
                     /translation="MPLEERVSLKLTKELYAKVENEAKKDKRSVNSMITIILEKFFAK
                     KKSD"
     gene            5078..5887
                     /locus_tag="PLF66_21970"
     CDS             5078..5887
                     /locus_tag="PLF66_21970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66771.1"
                     /translation="MSIETATQEKPIVQTSANNPNPIKPESRKRPWYKLPTGKQLGKR
                     IISPGNSIYVALLLSIVMVGVHITFLPTLFAQIYEMSYESAFMRSLILSLVASFSLLL
                     LKLKLKPTAFRLPWYFSKEGRFGKLTLFRANIPATLLMIVNIIFTFSGMLYDIKGQPQ
                     AVKEFIETVVVYNINFVIAVLPEVVVFYVLFDVYKNLRDRKKHTKLAPEKLAAMEKYI
                     NDNKNEPNFSLSFAAKVAGIQVTQAKPFLIEKYGAEEYERLSKLNPKSRKA"
     gene            5988..6152
                     /locus_tag="PLF66_21975"
     CDS             5988..6152
                     /locus_tag="PLF66_21975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66772.1"
                     /translation="MSIIILIEILIAIGLLYFIQAWFRESLQRRKDLKKIRKDIDLLQ
                     SMKSEIEEND"
     gene            6152..6340
                     /locus_tag="PLF66_21980"
     CDS             6152..6340
                     /locus_tag="PLF66_21980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66773.1"
                     /translation="MKILLIITAIALWVTISYQVETQFEEDEEEDKFERINNIRKIRN
                     FIIKQRLKGAKQWRNLHS"
     gene            6319..6642
                     /locus_tag="PLF66_21985"
     CDS             6319..6642
                     /locus_tag="PLF66_21985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66774.1"
                     /translation="MAQPTQLTQSELDFILEQRKLSPFTKIKVYLKKVLIHAFLALLI
                     TFSGLWFLVAFTDFKLPFLYSENKVEYQRLESGAVTRTTRKKVIVNLNPTIRKDGIVI
                     KEGKK"
     gene            6639..6902
                     /locus_tag="PLF66_21990"
     CDS             6639..6902
                     /locus_tag="PLF66_21990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66775.1"
                     /translation="MNMRIFKEWEELTTDEKEELRKESEQIIQKIKKEKGDQYYTDAL
                     YEIHQLKKELKEETEKSEFKGYLIGVFFVLSLVILNLLYEVLR"
     gene            6899..7177
                     /locus_tag="PLF66_21995"
     CDS             6899..7177
                     /locus_tag="PLF66_21995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66776.1"
                     /translation="MNEPLSLFENILILMIPIGFALLVKLFRSAESHAVNEVEQVQKE
                     LEGKVYHSITENHYHTHDNRTINIVVKDGSELKTVLSKKANQTKLLGE"
     gene            7180..7431
                     /locus_tag="PLF66_22000"
     CDS             7180..7431
                     /locus_tag="PLF66_22000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66777.1"
                     /translation="MIYWIILIICIIYFFLYIANKILFETRKNDFILDKIYGIETEGL
                     SLEERYALLARLENDIDKRQWDKEKGYDPKEGVFKIKKS"
     gene            7885..8076
                     /locus_tag="PLF66_22005"
     CDS             7885..8076
                     /locus_tag="PLF66_22005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66778.1"
                     /translation="MATVRISDNVLNELKVFLNPESKYVDVNYTKYVSDAVLEKMNRE
                     REVKNSKMKVAGLKKSVGK"
     gene            complement(8339..8944)
                     /gene="mtnB"
                     /locus_tag="PLF66_22010"
     CDS             complement(8339..8944)
                     /gene="mtnB"
                     /locus_tag="PLF66_22010"
                     /EC_number="4.2.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002975723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylthioribulose 1-phosphate dehydratase"
                     /protein_id="HMZ66779.1"
                     /translation="MVNPFDILINFSRSYYERGWMVATAGNLSIYDRASGKLWITASG
                     KHKGRLQENDFICINLEGKILEETENKPSAETSIHQVIYSKVPEANVVLHVHTVTSCK
                     LHFGVNKSNPVRRVHLPNVEILKAFGDFREEPNLSTIVTFNHGVVADIARDLEHALSF
                     RPSEVPFFLIENHGLTVWGKSVEDANKNLEAAEFILQVMVG"
     gene            complement(9208..9741)
                     /locus_tag="PLF66_22015"
     CDS             complement(9208..9741)
                     /locus_tag="PLF66_22015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acireductone dioxygenase"
                     /protein_id="HMZ66780.1"
                     /translation="MAKIIRKNKPEITDKGEVKKFLHDHGVEYDHWVVPDSAKIFTSK
                     QVLSDSEKENLLLTVNDRFEYLKNKEGYTTRDLVVLHPEVPGIHDMLAKFDKVHTHSD
                     VEVRYIIDGSGYFGFVAKEGNFLVHVFESDFISVPKGTQHWFTLDDKLRIKAVRYFKD
                     TSGWVPNYVEGAVASIG"
     gene            complement(9741..10307)
                     /gene="efp"
                     /locus_tag="PLF66_22020"
     CDS             complement(9741..10307)
                     /gene="efp"
                     /locus_tag="PLF66_22020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002733336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HMZ66781.1"
                     /translation="MVLGITEVRKGMVLKIENDIYTVIKSEFVNPGKGSAFIRTKLKG
                     VIKGSSIERTFKAAETLESVELEKRAMTLCYTEGDHIVFMDVNDYEQIHVPSEYVEDV
                     LPFMKEETQVEVTFYEGKPIGVIPPNFSILEVTYAEEGLKGDTSGTALKKVTVETGGE
                     INVPIFVKQGDKIKIDLRDLSYVERVNK"
     gene            10586..10753
                     /locus_tag="PLF66_22025"
     CDS             10586..10753
                     /locus_tag="PLF66_22025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66782.1"
                     /translation="MNIAQIETNLQKLVKSIKKTSFIYDLLLAYGQPKASIARLQKGG
                     LNLSKSEGEII"
     gene            10973..11176
                     /locus_tag="PLF66_22030"
     CDS             10973..11176
                     /locus_tag="PLF66_22030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66783.1"
                     /translation="MEKAQHQNENPADVKAAERMAKLYDELKKNNPIKTPEQVHNLNV
                     FLSRLLFCFFAEDTGIFEESQFA"
     gene            11169..11885
                     /locus_tag="PLF66_22035"
     CDS             11169..11885
                     /locus_tag="PLF66_22035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015679742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BRO family protein"
                     /protein_id="HMZ66784.1"
                     /translation="MPKIVSTKYVVVQSQLSEFIGQLKMESPDGKMRETDVANPEQLL
                     RLIQSIPSPRAEPFKQWLAKVGYERMQEISDPSQSIDRARENWQKLGRSEKWIQQRMT
                     GQETRNKLTDYWKQSGVEKKDEFALLTNIIHEEWTGLSVKKHKELKGLKSQNLRDHMS
                     EAELIFTALAELSTRQIAETEQAKGLSENADASKQGGRVAKNARKELEAKTGKSVVTG
                     ENFLPPKKETKQIKSQKKKK"
BASE COUNT         4395 a         1975 c         2081 g         3583 t
ORIGIN      
        1 aagccataat atcaatattg aagtagatcc caagcctctt cttatcgtcg ctggctgtat
       61 tggagttgga gtttttttat ttaaaaaata tggaaagaga aagaggtaaa caatgccaaa
      121 gcttattaga aatacagaag tagtcgaacg atgcagaaga ctctacaaag atggtcattc
      181 aatgccacag atagcagaaa aaattccaat agaatttcca tacataaaat caattcatcc
      241 taatacaatt ttcaatgcaa ttcattttgg agtaaacaca aggagtaaat taaatgttgc
      301 tagacctacc aacagctaaa aaagctttaa ctaacgtatg cactaacgca cccgcagatg
      361 caagatttca actaaagatt aaaaataaaa gtggttttag tataacgatt ccacaattaa
      421 aatttcctgg tttcttagaa cctggacaat atgaattttt tgcctttcaa tcaaatccag
      481 aaggagaatt tttagaagtt ttaagtgctg agttagttat tgaggcaatg gaagagtcta
      541 cacctaaagc ggttaattac gaacaacctc aaccagtatc tcagcaagca acccaacaaa
      601 caaatagaca ataccaagaa ttcgtttcac ctgattttat tctaaaatta ttagagaaag
      661 atcaagctgt attttcaaac gaaagaaaac tgcacgatga aaaagtttct atgattatgc
      721 aaaatcatac aaaggaaatt gagcgaataa aagaggcgca ccaaaaagaa atcacttctt
      781 taaaagattc tcattctata gaggttaata atttaaaaac tctaaatgaa aaaattgatg
      841 cagagagaga caagatcaga gagtcaattg aaaagcaatt acgcggagag cgcaaagaac
      901 aagcaagagc gggaaaaaaa tctgtgtggg atataatagc ggattttgta gagaaaaacc
      961 ctgatgaaat tttggctttt atccaatcaa aagatagagg aaattcacaa acagaaacaa
     1021 gatacagtaa tttacaggaa gcaattaagg aagcaagaga ggcaggatta taatgagtat
     1081 tgaagagcta gaaaagagaa tcaaaaaaga agagttaaag aataaagttt ttagatctat
     1141 ctttaaaact ttttatgaag aaattaaatc acaacttcct aaagcgtttg taaaagacgt
     1201 tgataatgcc tttgcacaac tagattcaat ggacaaactt taaaaagtaa tggcaaagaa
     1261 aaaagctatt tatattgaaa atggaagttt taaaatttta caagattctg attctctgat
     1321 gagttcagga tctggaattt ataactttac atcaagcaat aatagacttt actcttcgcc
     1381 ttcaaatgga actagccttt caacggctgc acttgtggct aatactcttt atgcaatgcc
     1441 ttttatttct gaaaaaagat ttattgtgtc acttctaaaa atgtcggtca cagtagcaag
     1501 tgcaggatct acagtaagag ctggaatata ttcagataat ggaagctgtt accctaattt
     1561 taaactaaat gatttagggc aagcaagcgg ggcaagtgtt gcagtaatac aattttcaaa
     1621 ttctttaccg gtgtcctatg ctgaaaattt attttggtta gtttgcgtag tttcagcagc
     1681 tattacagta aagggttttt cagtagcggg attaattcct ttacttggaa ctgattcaaa
     1741 tataggaaat gcacctggtt ttggatattc tatttctacg ggcgtatcta cattacccgc
     1801 aagttttcca acaggggcag caattagaac ggctgcacct ttgccctata tgggtttatt
     1861 tattacagga taggagttta aaaaaaatga gagatattaa attatgcgtt cccactttac
     1921 aagaagcctg gaaatattcg caagaatttt ggtcaaagaa ttttccaggt aagccccaac
     1981 ctattttaac ttgcacgctg agaacgattg cagaacaaaa agcgttattt gctcaaggca
     2041 gattaccatt atccgaaata aacaagctta gaaaaattgc aggtatggca gcgataagcg
     2101 caagcgaagg aaaaaagaaa gtcaccaatg cggatgctgg acaatcaaaa cacaatccag
     2161 ataaaaccgg attgtctagg gcttttgatg ttggatttct aactatctca agtggaaaaa
     2221 atcattctct agtttgggat gaaatcaatt ttaaagaatt caataaaatc atttccgcaa
     2281 aatttccatt agttcaatgg ggcgggaatt ggaaaagttt taaagattat ccacactttg
     2341 aggtaaagta atgaagtata aagaattaaa aaactacaag tatcaattag cagaaaacta
     2401 ttctattcaa acagaactaa aaccagtaaa tgcaattttc gaacctaaca aagaaaagcc
     2461 ttttattgaa ttggatacca atggagtttt aacaatattt tccggttatt cctgggacgg
     2521 tgcaagcggt attgctattg atactaataa ttttattcgc ggctctttag tgcatgacgc
     2581 tttttatcag ttaatgagaa aaagaaaact atcattagct cacagagatt atgcagatag
     2641 acttttacaa aagatttgca aagaagatgg aatgagtagt tttagagcga ataatgttta
     2701 ttacgcggtt aaaatttttg gcgaatcctc cgcagctcct acgagtaaaa aagagattca
     2761 agaaacaatt ctagaagctc cctgactgaa tgattgtatc cgaagatttg ataaatggaa
     2821 ttctacgaga gatagatgta atgtattcgg aaggactttt aaaaatagat gatgctgacg
     2881 atttatcaaa atcggtaggt tggatcaatg gaaagtcttt gaatgaatct aatgtatcgt
     2941 atgtgataga atgccttaga ggttataaaa aatttatgaa tgctgaagcg gtaacgagaa
     3001 taataaatct ttgccgtaaa actttcggga gttatatgta atatggaatc agaaaaaaaa
     3061 attgatctta aagaattcat ggaaagtttt aaagaacaca tagaaaatga atatccaaac
     3121 gcaccaaagg taaatattga aagcccgcta attcaaccgc aagaaaagaa ttttcgatac
     3181 tactttggaa tctttggaga aagtttaata cttataggga tgtatgctat tccattatca
     3241 gaagtatctc tagcggctta tggtctttat gtgcttttac ggtatgggat aatataaaat
     3301 gagtccatac aaagaaatga atgaaatagt tttctatttt gcaatcctat tccttgcatg
     3361 gtcggcttta atgtttaatt taggtcgatg tttttaagtg ctacgtagca cctggacttg
     3421 accgtttcct tattttttca ataaatttca ccataaccat tactagtttg gatctgattt
     3481 taatattgac aggaatgtca taaataacca cagtctaaat actatggcag aaaaagaaga
     3541 tttaagcaaa aaaggactaa cattttttac acgagccttt gaccacagaa cctatacggc
     3601 agatggggca ttttctcagt cattcaatga caaaataatt ttagattttt atgtagatcg
     3661 gattgtatac ccaacaaaga ttgtattcga tttagataaa gacgacaatt ttatagagcc
     3721 aggtaagttt tcaacttttg caaaaattac aacagaaaga aatatacagc attctttgac
     3781 tatcaattta aagactgcaa ttgaattaaa agaaacttta gaagaagcta taaatgattt
     3841 aattaatatg gaggaaaaag aaaatgaatc taactaaaaa tattagacct gctaaggcaa
     3901 attattcttt tccaacaaat cataatttaa gaattggtgg taaagggtct actcaatcac
     3961 aaacagaatt aaactttaat atgattttcg attttttaaa ttttatatta agtttcaatc
     4021 aagagcaaaa agaggaaata gttgagcagt ataacaattt acctgcaagg caaactttta
     4081 aaagaaatac aattgctata attcaaagct taaaaggatt tgaagtttta gagcctatta
     4141 aaattgaaat atctatagaa tatgaagaat atttagttga aatcccagag ctagaaattt
     4201 atgcatacga taaagatata catgaatgta ttaaagaaat aaaagaagat attatacgtt
     4261 tgtattatga tttaaaccga gaaagaaaag aaatattagg tgaaaaacca aaaaaatgga
     4321 aagatttctt aaattatagc attaaaaaaa tatgagcaaa agaaagttat tcactgaaaa
     4381 agaaatcaga gaaaaaatct taacaaaagt tgatcctcag aatattaata aagacggcaa
     4441 acattggata ggtgaaataa ttctaaacaa tgtatttatt aataaagtta aaattcctaa
     4501 ttcgcataaa gacacaatgt tccacaacaa gacaaaaaaa attgctgaag acctatgcat
     4561 cacaaatgat gaatttacag atttaataga ttgctcccta tcagggaaag actattacaa
     4621 aaaattagat gagaaattta atcccaaaga aaaaactctc tccgaagaaa acccctaatc
     4681 acttttcttc actgaatacc aaaaagcttt agcattcctg ctattataat tatcccgata
     4741 atcgagcaaa cacctatagc caaagaagcc caaataaatt tttcttcttt tttcaaaaga
     4801 aaaaccccct aatcactttt cttttttgcg aaaaactttt ctaggattat cgtaatcatt
     4861 gaattgactg atctcttatc tttcttagct tcattctcta cttttgcgta aagttctttt
     4921 gttaatttta aactaactct ttcttctaat ggcataataa agcatacctt ttatgccttt
     4981 tccataattg caatatattt ttattcttga cgaaactctg aaaggtatgt aatggtcata
     5041 ccaagtcata attagacaga ccaaaggagt attatcaatg agtatagaaa cagcaacaca
     5101 agaaaaacca atcgtgcaga caagcgcgaa caatccaaat ccaatcaaac cagaaagcag
     5161 aaaaagaccc tggtataaat tacctaccgg aaagcagtta ggtaaaagaa ttatcagccc
     5221 tggcaattca atttatgtag ctctactttt atcaatcgta atggtaggcg tgcatattac
     5281 atttcttcca actttatttg cacagattta tgaaatgtct tatgagtctg cattcatgag
     5341 aagtttaatt ttatctctag tggctagttt tagtttgctg ctattgaaat taaaattgaa
     5401 accgacagct tttagattgc cctggtattt ttccaaagaa ggcagattcg gaaagttgac
     5461 tttgtttcgt gcaaacattc ccgcaacttt attaatgatc gtgaatatca tttttacttt
     5521 ttctggaatg ctttacgaca tcaaaggtca accgcaagca gtgaaagaat ttattgagac
     5581 ggttgtagtt tacaatatca atttcgttat tgcagtctta ccggaagtcg tcgtgtttta
     5641 tgttctcttt gacgtttata aaaatttgag agatagaaag aagcatacaa aacttgcacc
     5701 tgagaaatta gcagcaatgg aaaaatatat taacgataac aagaatgaac ctaattttag
     5761 tttgtcgttt gccgctaaag ttgcaggtat ccaggttact caagcgaagc ccttcttaat
     5821 cgaaaaatat ggcgcggagg aatacgaaag actctctaag ttaaatccaa agagtcgcaa
     5881 ggcttaaatt ggtttgcaaa tttgcgtgta aaatattcgc aactttacac gcaaattatt
     5941 cgcaaatccc taagagattt acacgcaaat tattcgcaga gggtaaaatg agtatcataa
     6001 ttctaatcga aatcttaatt gcaatcggct tactttactt catccaagct tggtttcgtg
     6061 aatcactgca aagaagaaaa gatttaaaaa aaatcagaaa ggacatagac cttttacaat
     6121 ctatgaaatc agaaatagag gaaaacgact aatgaaaatt ttattaatta ttacagcgat
     6181 agctttgtgg gttaccatat cttatcaagt agaaactcaa tttgaagaag atgaagaaga
     6241 agataaattt gagcgaatta acaatattcg taaaataaga aattttatta ttaagcaacg
     6301 attaaaagga gcaaaacaat ggcgcaacct acacagctaa cacaatcaga attagatttt
     6361 attttagagc aaagaaagct atctcctttt acgaaaatca aagtctattt aaaaaaagtt
     6421 ttgattcatg cttttcttgc attactaatt acctttagtg gcttatggtt cttagtcgca
     6481 ttcactgact ttaaactccc atttctatat tcagaaaata aagttgagta tcaacgacta
     6541 gaaagtggcg cggtaactag aacaaccaga aaaaaagtaa tcgtaaatct taatccaaca
     6601 attcgaaaag atggaattgt aatcaaggaa ggtaagaaat gaatatgcgt atttttaaag
     6661 aatgggaaga attaacaaca gatgaaaaag aagaattaag aaaagaatct gagcaaataa
     6721 tacaaaaaat aaaaaaggaa aaaggggatc agtattatac agatgcttta tatgaaattc
     6781 atcaattaaa aaaagagtta aaagaagaaa ccgaaaaaag cgaattcaaa ggatatttga
     6841 taggagtatt ctttgtgctt tctcttgtta ttctaaatct actttatgag gttttaagat
     6901 gaacgaacca ctttctttat ttgaaaatat tttaattctt atgatcccta ttggctttgc
     6961 actcctagta aaacttttta gaagtgcaga aagccacgca gtaaatgaag tcgagcaagt
     7021 tcaaaaagaa ttagaaggga aagtttatca ttcgataact gaaaatcact atcacacaca
     7081 cgacaacaga actattaata ttgtcgtgaa agatggatcg gaattaaaaa ccgtcttgag
     7141 caaaaaagca aatcaaacca aacttttagg agaatgacaa tgatttactg gataatactt
     7201 attatttgca taatctattt ctttttgtat atcgcaaaca agattctttt tgagacaaga
     7261 aagaatgatt ttattttaga taagatctac ggaattgaaa ccgaaggatt aagcctagaa
     7321 gaaagatacg ctttattagc aaggttagaa aacgatattg ataaaagaca atgggataaa
     7381 gaaaaaggct atgatccgaa ggaaggagtc tttaaaataa aaaaatctta gtcggtgcta
     7441 cgtagcacct gcaaaaaatc agatccccga aaggggattt tttttgtcct catagatcta
     7501 caatttccga ccgcataaaa tcactcaggc taacaaaaaa agccgctcgg ctaccttacc
     7561 cctatttcct attctttcgc tctctggagc gtcttagaag cttttaaaaa gcccactctt
     7621 ctaatgagcg tcaagcgcat aagattaatg tatattattc tctattatag tatatttata
     7681 cattgtcact aaaagtacaa tgcatagtgc ctaaaaggag aatccattat ccttaaacaa
     7741 tcaatgcata gtgcctaaaa ggagaatcca ttatcctata aaggataatg ggagtttttc
     7801 agacaaaata ttttttaaat taagtattga ctttttttgt cgatgtgata catgtaacac
     7861 aaattactaa aaaggaggtt aaagatggca actgttagaa tatcagataa tgttttgaat
     7921 gagttaaaag tatttcttaa tccagagtca aaatatgttg atgttaatta tactaaatac
     7981 gtgtctgatg cggttttaga gaaaatgaat agggaacgag aagttaagaa ttctaaaatg
     8041 aaagttgctg gcttgaaaaa atcggtcggt aaatagtgag tttgcgtaag gtggaattat
     8101 ttttttcact gttctataac tcaccttacg caaggtgaac caaacgttaa tctgaggttt
     8161 gctcctagcg gagggcttgc taccgcagac tattgatttt aataattcat tagcattgtc
     8221 taatcttaaa aaaacatcat ttattcttta aattataaat tttgcgcaag gtgagttatt
     8281 aataacgaaa tgagtatttt tattttgtct gtattcatat agaatctctg acaataactc
     8341 accccaccat cacttgtaat ataaactctg cggcttctag atttttattc gcgtcttcta
     8401 ctgatttacc ccaaacagta agtccatgat tttcaattag aaagaatgga acttcgctcg
     8461 gacgaaaact aagtgcgtgc tctaaatctc gtgcgatatc cgcaacaact ccatgattaa
     8521 atgttacaat agtagaaaga ttgggctctt ctctaaaatc accaaaggct tttaggattt
     8581 ctacattggg taaatgaact cttctgacag gattggattt attgacacca aaatgaagtt
     8641 tacaggaagt aacagtatgc acatgcaata ctacatttgc ttctggaact ttagaataaa
     8701 tgacttggtg aatagaagtt tccgcgcttg gtttattttc tgtttcctct agaattttac
     8761 cttctagatt gatacaaata aaatcatttt cttgcagacg ccctttgtgt ttgccagaag
     8821 cggttatcca gagtttgccg ctagccctat cataaataga aagattacca gcagttgcca
     8881 ccatccagcc ccgctcataa taactgcggc taaaattgat tagaatatcg aaaggattta
     8941 ccacggatga acaccgatga acacggataa tttctcaatt agtgtgcggt tattaaagta
     9001 tttgaggtta gtagctctga gcatttcaac gaataaggct tcttctaaat aacaatttga
     9061 cgttcgcgca agattcacca tggcaaacaa atacctttca aatagaaact catattaagg
     9121 acataaaaat taattttcat aaaaaaccta atgaatcaaa acgcttatcg gtgctttccg
     9181 tgtccatcgg tggtaatctt ttaaaaatta ccctatacta gccacggcac cttcgacgta
     9241 gtttggaacc cagccgcttg tgtctttgaa atagcgaact gctttaatcc gaagtttgtc
     9301 gtctaacgtg aaccaatgct gtgttccttt cgggacagaa ataaaatctg attcaaaaac
     9361 atgcactaag aagtttcctt cttttgcaac aaaaccgaag taaccagatc catctatgat
     9421 ataacgaact tctacatcgg aatgagtgtg cactttgtca aatttagcaa gcatatcatg
     9481 aatacccgga acttctggat gcaatacaac taaatcacgt gttgtataac cctctttgtt
     9541 tttaagatat tcgaaacggt cattgacagt aagaagcaaa ttctctttct ctgaatcaga
     9601 gagaacttgc tttgaagtaa aaattttcgc agaatccgga acaacccagt gatcgtattc
     9661 tacaccatga tcgtgtagaa atttcttaac ttcaccttta tctgtaatct ctggtttgtt
     9721 tttacggatt atctttgcca ttatttattc actctctcca cataagaaag atctcgtaag
     9781 tcaattttga ttttatcgcc ttgctttacg aaaataggaa cgttaatctc tccacctgtt
     9841 tctacggtta cttttttaag agctgtccca gaggtatctc ccttgagacc ttcttctgca
     9901 taagtcactt ctagaattga gaagttaggc ggaatcacac caattggttt gccttcatag
     9961 aaagttactt ctacttgtgt ttcttctttc ataaatggaa gaacatcttc tacatattcg
    10021 gatggaacat gaatttgttc atagtcattc acatccatga atacaatgtg gtctccttct
    10081 gtgtagcata aagtcatagc tcttttttcg agttctacac tttctaaagt ttctgccgct
    10141 ttgaaggttc tttcaatgct agagcctttg atgacccctt ttaatttggt tcgaataaat
    10201 gcactaccct ttccaggatt tacaaattct gatttgatga ctgtataaat atcattttca
    10261 attttgagca ccatgccctt tcttacttct gtaataccga gtaccataaa acctgccgat
    10321 tattgattgt taaattaagg ttttttaact taatggcagt gtcaagtgaa aagaccctta
    10381 gcgcgaatct cctacatcta tttttattga aaaattccat caaattattc ccctttgcaa
    10441 tagagaagca aatcccctaa ccttttcaaa tttgtgagat attacagtta tattcccctt
    10501 tgtaaaaggg gagattttct tgacagaaaa aattactctg agtaagtcta atctgtccta
    10561 ttggaacaat aagatggatt aagcaatgaa catcgcccaa atagaaacca atttacaaaa
    10621 actcgttaag tcgattaaga aaactagctt tatctacgat ttgcttcttg cctatggaca
    10681 acccaaagct tcgattgcac gattgcaaaa aggggggctt aatctttcta aatcggaagg
    10741 cgagattatt tgaaagaaaa agcttttttt taaatcggca aagaatgtag atttacatac
    10801 actcatcgac gaagtcaaaa aagattctgc ggttactagg catgatccac gatttatcat
    10861 cataaccgat tttgaaacac tcttagccct tgatagaaaa actgcggata cgctagatat
    10921 acctctcata caaatcacaa agcattttga tttttttcta ccctgggcgg gaatggaaaa
    10981 agcccaacac caaaatgaaa acccagccga cgtaaaagcc gccgaaagaa tggcaaagct
    11041 ctacgacgaa ttaaaaaaga acaatccaat caaaacaccg gagcaagttc acaatctaaa
    11101 tgtatttttg tcacgactcc tattttgttt ttttgcagag gacactggaa tttttgaaga
    11161 aagccaattt gcctaaaatc gtgtccacta aatatgtagt agttcaaagt caactgtccg
    11221 aatttatcgg acagttgaaa atggaatctc cagatggaaa aatgagagaa accgacgttg
    11281 caaatccaga gcaactttta agattgattc agtcaattcc ttctccaagg gctgaaccat
    11341 ttaaacaatg gttagcaaaa gttggttatg agcgtatgca agaaatttca gatccaagcc
    11401 agagcattga tcgagcaaga gagaattggc agaagttggg tcgaagtgaa aaatggattc
    11461 aacagcgaat gactggacag gagacgcgaa ataaactaac agattactgg aagcaaagcg
    11521 gagtagaaaa aaaagatgag tttgcacttc ttactaatat tattcatgaa gaatggacgg
    11581 gactcagtgt taagaaacac aaagaactaa aaggattaaa gtctcaaaac ctaagagacc
    11641 atatgagtga ggccgaatta atttttactg cgttagccga actatccaca agacaaattg
    11701 cagaaacaga acaagctaag ggactttcag aaaatgctga tgcaagtaaa caaggtggaa
    11761 gagttgccaa gaatgctcga aaagaattag aagcgaaaac tggtaaaagt gttgtcacag
    11821 gtgaaaactt tttacctcca aaaaaagaaa caaaacaaat taaatctcaa aagaagaaaa
    11881 aataatgcca gatatagttc atgtaaccta aggacaaaca ggacaaagct ctaagactaa
    11941 cgctctcggg atgcgtgaaa tgcaagaaaa agcttatcaa gctagagatg ctgagtatat
    12001 tttactaaaa gctccgcccg cttcgggaaa atcg
//
LOCUS       DAOQOS010000143        12030 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_2710_length_12030_cov_5.778205, whole genome shotgun sequence.
ACCESSION   DAOQOS010000143 DAOQOS010000000
VERSION     DAOQOS010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 12030)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12030)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12030
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2710_length_12030_cov_5.778205"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..410
                     /locus_tag="PLF66_22040"
     CDS             <1..410
                     /locus_tag="PLF66_22040"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sugar nucleotide-binding protein"
                     /protein_id="HMZ66785.1"
                     /translation="LLRVQWLYGKNGKNFIDTMLKLSKEKSELNVVNDQIGRPTSTPY
                     LSELIVACLKKDLTGIYHLGPSDYCSWYDLASYVLRDTNCKVKPIPSSAYPTPAKRPL
                     YSVLGLEKIQTALKGSPLIKKTWKELADEYLSQ"
     gene            559..1998
                     /locus_tag="PLF66_22045"
     CDS             559..1998
                     /locus_tag="PLF66_22045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002972903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HMZ66786.1"
                     /translation="MVTIKIDGLEYKVNEKKNLIDAAKEVGVDIPYFCYHPSLSIVGM
                     CRMCLIEIEGVPKVQAACNTPVKEGLSVITKSQRVLEAREGTMEFLLANHPLDCPVCD
                     KAGECDLQDNAFSAGKGVSRFEFEKRNIPQEEIGDNLMINHNRCIVCYRCVRFEEELV
                     KESNLGLFQRGYHSIIGLANNKIITHNYQGALADLCPTGALLNHKTLFKSRVWWYKTA
                     KSICHGCSTGCNIETNVRDNKMYRYMARENAELGMYFLCDKGRFDIEWINENRLYAYY
                     EKGAPSESTKVIPKILEKIQSAKKIAVIGGAHESNENLALIKAQAEKLGKEVVFEARV
                     AKPQYSVPEQADFLMTTDAHPNTKGAYDLAFISTNGIDGVVKDFNEGKIDLIFVIKED
                     IPTNLKGSGTIVVLHTNITTSVKDADFAVPIKSFAEQIGSFTNKNGIKQNFELAMQPL
                     QGLPSSGDFFKRLNLELSIKKEELAIGHN"
     gene            1985..2512
                     /locus_tag="PLF66_22050"
     CDS             1985..2512
                     /locus_tag="PLF66_22050"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012390323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit I"
                     /protein_id="HMZ66787.1"
                     /translation="MATINVVNVAKRHKFAWYEKFYFYSISKGLFITLKHFIKAAFFK
                     GYVTVQYPDEKRAYSSRFRGMHSLKRDEKGRERCTACFCCMWICPADAITIEAAEVPT
                     ERQHLHPEDKYAKRFDIDLLRCIFCGLCEEACPKGAIYLDGGGEMAADNRYDLILTKE
                     RMTQKVGGPILGQRN"
     gene            complement(2574..3206)
                     /locus_tag="PLF66_22055"
     CDS             complement(2574..3206)
                     /locus_tag="PLF66_22055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66788.1"
                     /translation="MLPILMSIFLYLSIVLSISSSPIFFDIEENNSLKKRINGCSEFE
                     KRKEFQFKEKHFDIREFDNLNKELIKTAEISAKERISQIKCIQKLNLNDKEKEDMRKG
                     FDVYKENFLFLLEYYNRWIFSFDKSNFMTPTDRKATSEMIKLKYDLYNLLQVKTEDLL
                     FREELLKNRDFDSFEEQLASIYLSILKGNYEYYNNISSELRKEIFRKISD"
     gene            3470..5608
                     /locus_tag="PLF66_22060"
     CDS             3470..5608
                     /locus_tag="PLF66_22060"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HMZ66789.1"
                     /translation="MEKFKFSVRFSIVTFSTLAVLLFGGLVSFVSYLGSKNSVRFLVD
                     NLVEKASEHVIDKTVNYLDVAVVNTELNENLMNTGLVKIRKTRILEKYFQGVVETNPQ
                     LLSMFYGDEEGNFIMAKEMPDHSFSIQRIIRRGRKTKIIWEHQKEEWYEKEQFSNKIV
                     PTEEAFNPKTRPWYTEALASKTHAWTDVYVFFNDRKPGITCSRAVYDKTGRFIGVIGI
                     DISILELSEFLSNLKVGKNGKAFLIDAKNQIVAIPSSDPKDLEKIMQEETSPEGKKSI
                     KLLTADKIDNPLISSSFKFYEENKDKPKEDGINFFEYSFKGIDYIANYRSFKKDDIEW
                     KIAIVVPENDFMYIVHRNNKIILIISIVLVAFAVLFEIQFSQKLSKPLSLLSEEMRKI
                     QNFDLTSEIQVGSFLLEVDNMAQSFTKMRTGLRSFKKYVPDELVRELIALNKEAILEG
                     ERRNMTIYFSDIAGFTSISEKLQPEELVEQLSQYLGSASKIITEHHGTLDKYIGDAIM
                     AFWGAPTPIEDHALQACLSALEHKRVLKLANKELERLGKEPFNDRIGIHTGEVIVGNM
                     GSENRLNYTVIGDNVNLASRLESINKYYGTEIIISESTYELVQDFVEVRILDLVAVKG
                     KHNAIQIYELVCEKEETSLEIHKFILTFNEGVYQYRNRNFSDALKLFQETLRIKSSDL
                     ASRVYIERCENYIQNPPPENWNGVYESKSK"
     gene            5819..6553
                     /locus_tag="PLF66_22065"
     CDS             5819..6553
                     /locus_tag="PLF66_22065"
                     /inference="COORDINATES: protein motif:HMM:NF022112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HMZ66790.1"
                     /translation="MSSQEYQSTSSKSFLYYLFFVLIALIMIDKTLQRKQIKPNTNKF
                     TSQVSQISKLDIEDINPSTTNDASESGETSVPEVKAENNLKDLTESFFDETETLQGVY
                     FLKFYQAGNRNYSKLVKVQRKVKGSLKKRIKMALKELKKGPNLDEQDKGVLSVLPQNF
                     HYSKKMKLTEGILHISIDKSFQENAGKDLMQDRLDQLSHTLFEFKEIKGIKVYIDGKE
                     MHSMGKDNLKVPSILARSNFRKVIYL"
     gene            6576..6917
                     /locus_tag="PLF66_22070"
     CDS             6576..6917
                     /locus_tag="PLF66_22070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66791.1"
                     /translation="MTHSISLIKHWENTITIEIPEYSNFIPVTEYLKTIETLYDANPV
                     PSLTIIIADKESLSSVAYGFMMSLSKFSIKKGITLKLVCNERKILELIEILKIGQLFS
                     IYPTLEESTTD"
     gene            complement(6903..7652)
                     /gene="trpC"
                     /locus_tag="PLF66_22075"
     CDS             complement(6903..7652)
                     /gene="trpC"
                     /locus_tag="PLF66_22075"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008596931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HMZ66792.1"
                     /translation="MNPVLQKIVLQKESEISQTPFYKDLPKPKYSLYNSLKSSSHSII
                     AECKKASPSMGIIRENYNPLEIAKIYAKSGARAISVLTDKNFFQGSLEDLKAVSENVE
                     IPVLRKDFVIHEKQIREAAIFGASAILLIVRILKPYQLQNLLEFAYSIGLEALVEIHT
                     KEEAKIAEEAGAKIIGINTRDLDTFKIHPNLIEEVSPTLSKDTLIVGESGIKNKQDYL
                     NMKKYVQSVLIGTYFMQKENIELAFKELISG"
     gene            complement(7671..8006)
                     /locus_tag="PLF66_22080"
     CDS             complement(7671..8006)
                     /locus_tag="PLF66_22080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004458108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HMZ66793.1"
                     /translation="MLKNTIKGDILVVYLEGRLDVSVANEVEEGLAEIIDNGGHKKVL
                     LNMEGIEYMSSSGFRACISTLRKLNSKEGVLKMCNIKPAVKRIFDVIELTSLFDIYPT
                     EADALADFK"
     gene            8082..9416
                     /gene="murD"
                     /locus_tag="PLF66_22085"
     CDS             8082..9416
                     /gene="murD"
                     /locus_tag="PLF66_22085"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HMZ66794.1"
                     /translation="MNLDKRFREAKRILVLGGGITGKSVIKFLRQYNVEILLIDAKED
                     LSLEKITYFNESADYEKLGKIDLAIKSPGFKPTHPLVERLRKESIPILSEIEIAREFF
                     SGKIIGITGTDGKSTTTALTYHILSKSFPNSKMGGNIGLPFIEFCQENLDFAVLELSS
                     YQLDDSNFLNLDYSALLNIAPDHLERHGTMENYLEAKKKIINRKDSNSFFITNTRLID
                     KLKLESLNLKNKVLLFGDSNSDAQIYFDKKEIKTKLNTYSTKSFPLQGKHNLENLAAS
                     ILLCESCGCTKEKIELAIADFTGLLYRFQKVKEWNGIPFINDSKSTNIHSMLSGLSGI
                     DRQSPIILIIGGKQKQEDLSPFIERLKSLTAKVFLIGDARLEWEKELSHVLKENLIVL
                     ENLGEAVKTIRETINPNETKMVIFSPACASFDQYKNFEERGKHFNDLLDQFF"
     gene            complement(9399..11333)
                     /locus_tag="PLF66_22090"
     CDS             complement(9399..11333)
                     /locus_tag="PLF66_22090"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF002730.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HMZ66795.1"
                     /translation="MNSFIRLATIVPEIKVGDIDFNTKEILNSLTKAKENEAEIALFP
                     ELSITGYSCADLFYQSKLRKNSIESLLEITEHTGKLKLTAVIGLPIESNGKLFNCAAF
                     LANGKILGIVPKSYLPTTGEFYEERWFSSESDRITDKFRLNGEEIPFGANLIFKAENL
                     DSLKIGIEICEDLWSVIPPSSEQSIAGVNLILNPSASNEILGKFEYRKELIKQQSARC
                     LAIYAYASAGPNESSTDVVYSGHALICENGSILSESNRYSFQTTYLYADVDLERTEND
                     RIRNSSFSKSKIKSTYREVLFPLNRSKKEKLLRFISSTPFVPSDLQKRNQNCEEIFQI
                     QSTGLAKRLKHTNVQKVVLGLSGGLDSTLAVLVCVKAFEILGLEKRGILCITMPGFGT
                     TDRTRSNAEMLAIELGASLKIISIADSVLQHFKDIEQDPNLQDITYENSQARERTQIL
                     MDVANKENALVIGTGDMSELAIGWCTYNGDQMSMYGVNAGIPKTLVRYLIEWRADAEY
                     QNKIGQILHDICDTPVSPELLPAQNGEIVQKTEEVVGPYLLHDFFLYYMLRMNFSPTK
                     IYLLAEIAFNGKFSKEEILKWMKVFYSRFFSQQFKRSAMPDGPKVGTVALSPRGDLRM
                     PSDASGKLWLEEINKLKELI"
     gene            11688..>12030
                     /locus_tag="PLF66_22095"
     CDS             11688..>12030
                     /locus_tag="PLF66_22095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HMZ66796.1"
                     /translation="MQSKFKPPFLLRSATVQTILASLKFEKLKKNPMFERAEMHLIKA
                     GKNIRLIGYLSKQIHGDSKGLVLLLHGWEGHVNSAYILKTASYLYNNGFDIFRINLRD
                     HGGTHHLNDELF"
BASE COUNT         4264 a         1980 c         1951 g         3835 t
ORIGIN      
        1 tgctccttag agtgcagtgg ctttatggaa aaaatggaaa aaacttcata gatacaatgc
       61 ttaaactttc aaaagaaaaa tcagaactaa acgtggtaaa cgaccaaatt ggaagaccta
      121 cttcaacacc atatttatct gaactaattg ttgcttgttt gaaaaaggat ttaaccggta
      181 tttatcatct cggtccatcc gattattgtt cttggtatga tctagcctcc tatgtattaa
      241 gagatacaaa ttgtaaagta aagcctattc cctcttctgc ttatccgaca cctgctaaac
      301 gtcccttgta ttcggttctc ggactggaaa aaattcaaac cgctctcaaa ggaagtccac
      361 ttataaaaaa aacttggaaa gaattagcag atgaatattt gagccaataa ttggtgtgtt
      421 tcgaataacc tttgataatg aaaggaaaat atttcattcc aagaaagaag aacttttgta
      481 agccgttttt tctacttgaa gaattttagg catttatgaa tagtcaaatc tatacgaata
      541 ttaaatcgga gtaactctgt ggtaacaatc aaaattgatg gtttagaata taaagttaac
      601 gaaaagaaaa atctcatcga tgcagcaaaa gaagtaggag tagatattcc ctacttttgt
      661 tatcatccat ccctaagtat tgtgggcatg tgtcgtatgt gcctcattga aatcgaaggt
      721 gtacctaaag tgcaagctgc ttgcaacacc ccagtaaaag aaggactttc agtcatcaca
      781 aagagtcaaa gggtcttaga agcaagagaa ggcacaatgg aattcctact tgcaaaccat
      841 cctcttgatt gtcctgtttg cgataaagca ggagagtgtg atttacaaga caacgctttc
      901 tccgcaggca agggagtttc tcgatttgaa ttcgagaaaa gaaatattcc acaagaagaa
      961 attggtgata acctgatgat caatcacaac cgttgtattg tttgctaccg ttgtgttcgc
     1021 tttgaagaag agcttgtaaa agaatccaat cttgggttat ttcagagagg ctaccactct
     1081 attataggtc ttgcgaataa taaaatcatc acacataatt accaaggagc tcttgctgac
     1141 ctttgtccaa ctggagcact tctaaatcac aaaacattat ttaagtcaag agtttggtgg
     1201 tataaaacag ctaagtccat ttgtcatggt tgtagcactg gttgtaatat tgaaactaac
     1261 gttcgcgata ataagatgta tcgttatatg gcaagagaaa atgcggaact tggaatgtat
     1321 ttcctctgcg ataaaggtag atttgatata gagtggatca atgaaaacag actctatgct
     1381 tattatgaaa aaggtgctcc aagtgagagt acaaaagtga ttccaaaaat cttagaaaaa
     1441 atccaatcag ctaaaaaaat tgccgtgata ggtggagccc atgaatccaa tgaaaaccta
     1501 gcacttatca aagcacaagc tgagaaactt gggaaagaag tcgtttttga agctagagtt
     1561 gcgaaaccac aatactctgt cccagaacaa gcagactttt taatgactac agatgcacat
     1621 ccaaatacaa aaggtgcata tgacctagca tttatttcta caaatggaat agatggagta
     1681 gtaaaagact tcaatgaagg gaaaatagac ctaattttcg taatcaaaga agatatacca
     1741 accaacctaa aaggttccgg aacgatagtt gtgcttcaca caaatattac tacatctgta
     1801 aaagatgctg actttgctgt tccaatcaaa agctttgcag aacaaattgg ttcctttaca
     1861 aataaaaacg gaatcaagca gaattttgaa ttagcaatgc aacctttaca gggtctacca
     1921 tcttctgggg acttcttcaa acgtctaaat ttagaacttt ccattaaaaa agaggagtta
     1981 gcaattggcc acaattaatg ttgtaaatgt agcgaaaaga cacaagttcg cttggtatga
     2041 aaaattttat ttctactcaa tatccaaagg tttgtttatt accttaaagc attttatcaa
     2101 agcagctttt tttaaagggt atgtaacagt tcagtatcct gatgagaagc gtgcctactc
     2161 ctctcgtttt cggggaatgc attctttaaa aagagatgag aaaggaagag aaagatgcac
     2221 tgcttgcttt tgttgtatgt ggatctgtcc tgcagatgca atcacaattg aagcggctga
     2281 agttccgaca gaaagacaac atcttcatcc agaagataaa tatgcaaaac gttttgatat
     2341 agatcttctt cgttgtattt tctgcggact ctgtgaagag gcttgcccga aaggagcaat
     2401 ctatctagat ggcggcggag aaatggcagc tgataataga tatgatttaa ttctgacaaa
     2461 agaaagaatg actcaaaaag tgggaggtcc gatcctagga caaagaaatt aattcttcca
     2521 cctaacgttt cttttctaaa aagactgccc tgtaagaagg cagtcttttt tatttaatca
     2581 gagatctttc taaaaatttc ctttctcagt tcagaactaa tattattata atattcataa
     2641 tttcccttca atattgagag ataaatagaa gctagttgct cttcaaaaga atcaaaatct
     2701 ctgtttttta gcaattcttc tctaaacagt aagtcttctg tttttacttg aagtaaatta
     2761 tataaatcat attttaattt aatcatttcg gaagtggctt ttctatctgt tggtgtcata
     2821 aaattagatt tatcgaaaga gaaaatccat cgattgtagt attccagcaa aaataaaaaa
     2881 ttttctttat aaacatcaaa gccctttcgc atatcttctt tttctttatc attcaaattt
     2941 aatttttgaa tacatttaat ttgagagatt ctttcttttg cacttatctc tgcagtctta
     3001 atcagctctt tatttagatt atcaaactct cttatgtcaa aatgtttttc tttaaactga
     3061 aactcctttc tcttttcgaa ctcagaacag ccattgattc gctttttcaa agaattattc
     3121 tcctcaatat cgaaaaaaat tggagaggaa gatatagaca aaacaatact taaatataag
     3181 aatatggaca ttagaatcgg aagcataaac tctaaaaata aaaaataaaa atgatttgca
     3241 agtgtaaaac tatatagaaa caaaaggaga atctaaattt caattctaaa acacgcctta
     3301 tgcagaacaa agacctctaa attctctaaa atccaaaaaa tccatatcag agtaatacaa
     3361 aaatccattc agaaagattt ggaaacttat ttcagaaaat cctcaataaa ttcacttgct
     3421 attaaaataa caaaaaaagt ataccttact aagaggattc tattttttga tggaaaagtt
     3481 taagttcagt gttcgtttta gtatagttac attttctacc ctagccgttc tactttttgg
     3541 tgggttggtt agttttgttt cctatctcgg tagtaaaaac tcagttcgtt ttttagtaga
     3601 taatttagtc gaaaaagcat cagaacatgt aattgataaa acagtcaatt atctagacgt
     3661 agctgttgta aatacagaat taaatgaaaa tttaatgaat actggtctag ttaaaattcg
     3721 aaaaacaaga attctagaaa aatattttca aggcgttgta gaaacgaatc cgcaactact
     3781 tagtatgttt tatggggatg aagaaggaaa cttcattatg gcaaaagaaa tgcctgacca
     3841 tagctttagc atccaacgta ttatcagaag aggacgaaag accaaaatta tttgggaaca
     3901 tcaaaaagaa gaatggtatg aaaaagaaca attttcaaat aagattgttc caacagaaga
     3961 agcttttaac cccaaaacaa gaccttggta tacagaggca cttgcttcta aaacacatgc
     4021 ttggacagat gtatatgttt tttttaatga tcgcaaacca ggaattacct gtagccgagc
     4081 agtttatgat aaaacaggtc gatttattgg tgtaattgga attgatatca gtattctaga
     4141 actttcagaa tttttaagta atttgaaagt tggtaaaaac ggaaaagctt tcttaataga
     4201 cgcaaaaaat caaatcgtag caattccttc ttcagatcca aaagacttag aaaaaataat
     4261 gcaagaagaa acctctcccg agggtaagaa atctattaaa cttttaacag cggataagat
     4321 agataatccg cttatctcta gttcatttaa attctatgaa gagaataaag ataaaccaaa
     4381 agaagacggt ataaattttt ttgaatacag ttttaaggga atcgactata ttgcaaacta
     4441 tcgatccttc aaaaaagatg atatcgaatg gaaaatagca atcgtagttc cagagaatga
     4501 ttttatgtat atcgttcacc gaaacaataa gattatttta attatatcta tagtgctagt
     4561 tgcttttgca gttctatttg aaatacaatt ttcccaaaaa ctatctaaac cgttatcact
     4621 actatccgaa gaaatgagga aaatacagaa ttttgatcta acctctgaaa ttcaagttgg
     4681 gtctttttta cttgaagtgg acaatatggc tcaatctttt actaagatga gaactggtct
     4741 tcgttctttt aagaaatatg ttccagatga attagttaga gaactcattg ctctaaataa
     4801 agaagccatc ttagaaggcg aacgtagaaa tatgacaatc tatttctctg atattgctgg
     4861 atttacttct atttccgaga aactacaacc agaagaacta gtagagcaac tcagccaata
     4921 tcttggaagc gcaagtaaaa tcattacaga gcatcatggg actttggata aatacattgg
     4981 agatgcgatc atggcttttt ggggggcacc tactccaatt gaagatcacg ccttacaagc
     5041 atgtctttct gcattagaac ataaaagagt actaaagctt gcaaataaag aattggaaag
     5101 attgggaaag gaaccattta acgatcgaat tggaattcat acaggcgaag taattgttgg
     5161 aaatatgggc tcagaaaaca gactcaacta cactgtgatt ggagataacg ttaatcttgc
     5221 cagtcgttta gagagtataa ataaatacta tggaactgaa atcattatta gtgaatcaac
     5281 ttacgaacta gttcaagatt ttgttgaagt aagaatttta gatttagttg cagttaaggg
     5341 aaaacataac gctatacaaa tttatgaatt ggtttgcgag aaagaagaaa ctagtctcga
     5401 gattcataaa tttattctga catttaacga aggagtttat caatatagaa atcgaaattt
     5461 ttctgatgca ttgaaactat ttcaagaaac acttagaatt aaatcatctg atcttgcttc
     5521 aagagtatat attgaacgtt gtgaaaatta tatccagaat ccaccgcctg aaaattggaa
     5581 tggagtttat gaatcaaaat ccaaataatt ttattttgtc gcatttcaag tatattagaa
     5641 cattataata tacttattaa ctgaagagtc aaaatttcca aatacaaatg aaacataatc
     5701 aatagtatta tgtttcattt gatgcttttt ttaaattttt tttcaaaata ttttctatta
     5761 tctttaaaat gaaaatttag tttctcaata gtgaactttc ggtttccgaa aataaagtgt
     5821 gtcatcacaa gaatatcaat ctacatcctc aaaatctttt ttatattatt tattctttgt
     5881 attgatcgct ttgataatga ttgataagac tttgcagagg aagcaaatta aaccaaacac
     5941 aaataagttc acttcacaag taagtcaaat ctctaaacta gatatagaag acataaatcc
     6001 atctacaacg aatgacgcat ccgaatcagg agagacaagt gttcctgaag taaaagcaga
     6061 aaataattta aaagatctaa ctgaatcttt ctttgatgaa acggaaacat tacaaggtgt
     6121 ttacttctta aaattttacc aagcaggaaa tagaaattat tccaaacttg taaaagtcca
     6181 gagaaaggta aaaggtagtc ttaagaaaag aataaaaatg gctttgaagg aactgaaaaa
     6241 aggtccaaat ctagatgaac aagataaagg tgtattaagt gttcttcctc aaaatttcca
     6301 ttattcaaaa aaaatgaagc ttacagaagg gattctccat atttctattg ataaaagttt
     6361 tcaagaaaat gcaggaaaag acctaatgca agatagacta gaccaattga gtcacactct
     6421 attcgagttt aaggaaatca aaggaattaa agtttatatt gatggaaaag aaatgcattc
     6481 aatgggaaaa gataatttaa aagttccatc cattttagct agaagcaatt ttagaaaagt
     6541 tatatattta taagccttcg aaattttgcc aattcatgac tcattctatc tccttaatca
     6601 aacattggga aaatacaatt acaatcgaaa ttccggaata ctctaacttc attcctgtta
     6661 cagaatattt gaagacaatt gaaactcttt acgatgctaa tcccgttcct tctctaacta
     6721 taatcatagc agacaaagaa tctctatctt cagtggctta tggatttatg atgtcattgt
     6781 ctaaattttc gattaaaaaa gggatcactc taaaactagt ttgtaatgaa aggaaaatac
     6841 tcgaactaat tgaaattcta aaaattgggc agttattttc tatctatcca acgttagaag
     6901 aatcaaccac tgattaattc tttaaaagcc aattcaatat tttctttctg cataaaataa
     6961 gttccaatta aaacagattg aacatatttt ttcatattca gatagtcttg tttgtttttt
     7021 ataccagatt cccctactat cagcgtatct ttagaaagag ttggagatac ttcttcaatt
     7081 aaatttggat gaattttgaa agtatctaag tctcttgtat taattcctat aatttttgca
     7141 ccggcctctt ctgcaatctt ggcttcttct tttgtgtgaa tctctactaa cgcttctaaa
     7201 ccaatagagt aggcaaattc taataggttc tgtagttgat atggctttaa aatcctaaca
     7261 atgagtaaga tggcggaggc tccaaagata gctgcttctc gaatttgttt ctcgtgaatg
     7321 acaaaatcct ttcgaagaac agggatttca acattctcac tgactgcttt taaatcttcc
     7381 aagcttcctt gaaaaaaatt tttatctgtt aaaactgaga ttgctctagc tcctgattta
     7441 gcatagattt ttgcaatttc gagtgggtta taattttctc tgatgatccc catggaagga
     7501 cttgcctttt tacattctgc aatgatagaa tgagaactag atttcaggga gttataaaga
     7561 gaatatttag gttttggtag gtctttgtaa aaaggagtct gtgagatttc agactccttc
     7621 tgtaaaacaa ttttttgaag aactggatta atagtattta aaaaatctga ttatttgaaa
     7681 tcagcaagtg catctgcttc tgttggataa atatcaaata atgatgtcaa ttcgattaca
     7741 tcaaatattc tcttaacagc cggcttgata ttgcacattt ttaaaacgcc ttcttttgaa
     7801 tttaattttc ggagtgtaga gatacatgct ctaaatcctg aagaagacat atattcaatg
     7861 ccttccatat tcaaaagaac ctttttgtga ccgccattgt ctatgatttc tgcaagaccc
     7921 tcttctactt cattggctac agaaacatcc aaacgtcctt ccaaataaac tactaatata
     7981 tcgcctttta ttgtgttttt aagcaaagta agtttcctaa aaaataatat atacaattac
     8041 cctaagacta aaattgtgtc aatgaaactt ttataaaaaa tatgaactta gacaaacgat
     8101 ttagagaagc aaagagaatt ctagtcttag gcggtggcat cacaggaaaa tctgtcatca
     8161 agtttctaag acaatacaat gttgaaatct tattgataga cgcgaaagaa gatttaagtt
     8221 tagagaaaat aacttatttt aatgagagcg cagattatga gaagctaggt aaaattgatc
     8281 tagcaattaa gagtccaggt tttaaaccta cccatccttt agtggaaagg ctacgaaaag
     8341 aaagcattcc aattctaagt gaaattgaaa ttgcaagaga attcttttct ggcaaaatta
     8401 taggcatcac tggaactgat ggaaaatcaa caaccacagc acttacctat catattctat
     8461 ctaaaagttt tcctaactcg aaaatggggg gaaatatcgg actccctttc atagaatttt
     8521 gccaagagaa tttagatttt gctgttttag aattaagcag ctatcaacta gatgattcta
     8581 attttctcaa tttagattat tccgcccttt taaacatagc accagatcac ttagaaagac
     8641 atggcacaat ggaaaattat ttagaggcaa aaaagaaaat tatcaatcga aaggactcga
     8701 attctttttt tataaccaac acacgtctca tcgacaaatt aaaattagaa agtctaaacc
     8761 taaaaaacaa agtgcttcta tttggagatt cgaattctga tgcacaaatc tattttgata
     8821 aaaaagaaat taaaacaaaa ctaaacacct actctacgaa atcttttcca ctacaaggaa
     8881 agcacaatct tgaaaatcta gcagcaagta ttttgctttg tgaatcttgt ggttgcacaa
     8941 aagaaaagat tgaattagca atcgcagatt tcacaggact gctatatcga tttcagaaag
     9001 tgaaagaatg gaatgggatt ccatttatta atgactctaa gtccacaaac attcatagta
     9061 tgctttctgg gctatcagga atcgacagac aatcaccgat tatcctcatt attggaggca
     9121 agcaaaagca agaagatttg agtcccttta tagaaagatt aaaatctctt acggcaaaag
     9181 tctttttaat tggggatgct agacttgaat gggagaaaga actttcgcat gtattaaaag
     9241 aaaatttaat tgtattagag aatctaggtg aagcagtaaa aacaattcgt gaaacgataa
     9301 atccaaatga aactaaaatg gtaatttttt ctcctgcgtg tgccagtttt gatcaatata
     9361 aaaactttga agaaagaggg aaacatttca acgatttact agatcaattc ttttagttta
     9421 ttaatttcct ctaaccacag tttaccgctt gcatcgctag gcatacgaag atcccctctt
     9481 ggagataggg cgactgttcc gactttgggt ccatcaggca ttgcagatct tttaaactgt
     9541 tgtgaaaaaa atcgactata aaaaaccttc atccatttta aaatttcttc ctttgagaat
     9601 ttaccattaa atgcaatctc ggcaagaaga taaatcttgg tcggactaaa attcattcta
     9661 agcatataat acaaaaagaa atcatgcaat aaataaggtc ctactacctc ttctgtcttt
     9721 tgcacaattt ccccattctg cgcaggaagt aattctggtg atacaggtgt atcacaaata
     9781 tcatgcagaa tttgtcctat tttattttga tactctgcat cagccctcca ctctatcaaa
     9841 taacgcacta aagtctttgg aatacctgca ttgactccat acatagacat ttgatctcca
     9901 ttgtatgtgc accatccaat cgcaagttca gacatatccc ccgttccaat gacaagggca
     9961 ttctctttat ttgccacatc cattagaatt tgtgttctct ctcttgcttg ggagttttca
    10021 taggttatat cctgtaaatt cggatcttgt tcaatatctt tgaaatgttg caaaacagaa
    10081 tctgcgattg aaatgatttt caaactagct cccaattcta tcgcgagcat ctcagcattt
    10141 gatctggttc gatctgttgt tccaaagcct ggcatcgtga tacaaagaat tcctcttttc
    10201 tctaaaccaa gtatttcgaa tgcttttaca cagactaaaa cagcaagtgt agaatcaaga
    10261 cctcccgaaa gacctaagac tactttttgc acatttgtat gttttaatct ttttgcaaga
    10321 ccagtagatt gaatttgaaa tatttcttca caattttgat ttcgtttttg taaatcggag
    10381 ggaacaaatg gagtgctaga aataaaacga agtaatttct ctttcttaga tcgattgagt
    10441 ggaaataaaa cttctcgata ggtagattta atttttgatt tagagaaact gctatttcga
    10501 attctatcat tctcggttct ctccaaatca acatctgcat ataaataagt agtttgaaaa
    10561 gaatagcgat ttgattcgga taaaatactt ccattttcac aaattagagc atgaccagaa
    10621 taaactacat ctgtgcttga ttcatttgga ccagcagatg cataggcata gattgcaaga
    10681 catctcgcag attgttgctt aattaattct ttacgatact caaatttacc cagtatttca
    10741 ttgctcgcag aaggatttaa aattagattc acacctgcaa tggattgttc gctacttggc
    10801 ggaattacag accaaagatc ctcacaaatt tcaataccta ttttaagact atctaaattc
    10861 tctgctttaa agattaaatt tgcaccaaaa ggaatttcct ctccattcag cctaaattta
    10921 tccgttatgc gatcactctc acttgaaaac catctctctt cataaaattc acctgttgtt
    10981 ggtaaataag atttcggaac tatgcctaaa attttaccat ttgctaaaaa cgcggcacaa
    11041 ttaaatagtt ttccattgga ttcaatggga agaccaatta cggcggttaa ttttaatttg
    11101 cccgtatgct ctgtaatttc tagtagtgat tctatactgt tttttcttaa cttggactga
    11161 taaaataaat ctgcacaaga atagcctgta atagataatt ctggaaataa agcaatttcc
    11221 gcttcatttt cttttgcctt tgttaaagaa tttaaaattt cttttgtatt aaagtcaata
    11281 tcaccgactt ttatttctgg aactattgtc gccaaacgta tgaagctatt cattttttag
    11341 aatttccttt ttaaaaatta atcatgaaaa ataggagaag gttttaatct atatttcaat
    11401 acagtctctc tatttttaaa ttgggtcaat aacttttgat cttcaaaaag aaagaaaagt
    11461 aagaacaagt tggtaaagct aatatagatt ttttgctaca caatacaatt tataaaattt
    11521 cttacataat caatagaaac caaagcagta taaccacaat tctaaggttt ttataaggtc
    11581 agtatttttt gaatttcata ttaattagtt tcttatagac aacacgatta tgaaagtaaa
    11641 actaggtaaa taaaaagttt atttatttac cataaagtat ataaaacatg caatccaaat
    11701 ttaaacctcc attcctactc cgatctgcaa ccgttcaaac aattcttgcc agtttaaaat
    11761 ttgaaaagct caaaaaaaat cctatgtttg aaagagcaga aatgcattta atcaaagcag
    11821 gaaaaaatat caggctcatt ggttatctat caaaacaaat tcatggagac tctaaaggtc
    11881 ttgtattact tttacacggt tgggaaggcc atgtaaattc tgcttatatt ttaaaaactg
    11941 cttcctatct ttataacaat gggtttgata tttttagaat taatttgaga gatcatgggg
    12001 gaacccatca tctaaatgat gaacttttta
//
LOCUS       DAOQOS010000144        11993 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_2724_length_11993_cov_5.507036, whole genome shotgun sequence.
ACCESSION   DAOQOS010000144 DAOQOS010000000
VERSION     DAOQOS010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 11993)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11993)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11993
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2724_length_11993_cov_5.507036"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            52..1104
                     /locus_tag="PLF66_22100"
     CDS             52..1104
                     /locus_tag="PLF66_22100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66797.1"
                     /translation="MKKICSLFLITFLLQADSNLNIRHVTIADIVYQPHTEEGYLQGW
                     NFFFRNSEYNIIATFLVSNLGPNDFNNGVSIAIESKKTGSLFITKEFGQKDLNVENGI
                     NLKMYNNTLKYESGKFELSQYYDDIKIFLKFESDGLGATLSGGKHFVKGNDKFVRADI
                     PFSFVRATGYLDFKGEIVELVGVGGLEHLITNYEVYKYSSKWEILRASNKEGMKLYTG
                     GFHGIKKNADDFFRTIVIQNPKGEIILSGIVTKSEIISSELDKFSGYVLPIKEKIYIG
                     DNGCYVQSERLYSVGKINILSNISAVLRFFIKLIFANPYQVNFLTKLNVVCNDSLKIS
                     TSDFIGIHSYYLINPK"
     gene            1324..3864
                     /locus_tag="PLF66_22105"
     CDS             1324..3864
                     /locus_tag="PLF66_22105"
                     /inference="COORDINATES: protein motif:HMM:NF012580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HMZ66798.1"
                     /translation="MKIDLAHCEREPIHIPDLIQPHGYAIVYEKESLIITRCSDNINL
                     IEKDLFIKFLDKRITDVIPYPLFEKIQKKTNFSLHKRDLLLNESLPTLNIQNAAIFVS
                     DAFEEVIIEIIPTFVFTDDSLLDTELNDMIERLIKSPTISSMFEVAVKDIKKLLGYDR
                     VMVYQFDSEFNGEVLSEAKEDGMESYLNLHYPATDIPAQARELYKTNMIRTIVDNHYI
                     PVKFISSQKNPLNMSHSYLRSVSPIHLEYLENMGVRATLTVSIMVEGKLWGLISCHHR
                     EIFFPDIKKQNIAQIFGSILGGIIQMREEGEREKKSSGLLSRLDLVMEMLLFQNTGSE
                     LLTLIRSKIVLLQSIFSSDGFMVFINGSIIAHNFPLSESQILELIEKLDPFLTEKIFY
                     TDSLTETFPNLPEIVLKLCAGLMVVKLDSKPRSYWIWRRVEKTQTIAWGGNPNQKAIL
                     NQKGEISPRKSFEKYNQIVTKKSVPWNRSEKDFYIYLIPRLHRLYEIFESNQEIESQK
                     RHILHIEEERAKHFEELIEMLVELIEMRDSYTGNHTKRVADYSVAIAQELKIKSDEIR
                     LLREASILHDIGKIIIPDSILLKPGKLSYKEYELIKQHVMVGHTILDKIDYYKPIAYI
                     ILHHHEKFDGSGYPFGKKGSDIPYLSHIMMVADSFDAMTTNRIYQARKSNEEARKELL
                     KYKGIWYHPDVVDAAIRVIQKNEKEDLKTTQLPITQIEQERFAYFFKDQLTGVYNATY
                     LWMGINDLISGISSEHFLLLELHGMSDLNSQLGWNKGNEVIQEFAGYLLEQVKGDKVY
                     RVFGDDFIVAFDTIEEKEKFLKNCKTFRIENVFSECKNIEKDKFKEIL"
     gene            4055..5893
                     /locus_tag="PLF66_22110"
     CDS             4055..5893
                     /locus_tag="PLF66_22110"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohemerythrin"
                     /protein_id="HMZ66799.1"
                     /translation="MNIKKIIKTIRESKIQVRLLITYIFFLLVLMSFPIFLFYNNYQS
                     KSLSQNQLQLIEDHMKNLEQYEKDLNLNLQYEKSLKIKSEEIQRETNGHLNKNLFYIL
                     IFTIIIIIGSFITVMVILKDFIKPLKEAISAAERLSSNDMNFEVNDRFMDEMGSLLHH
                     IKIVRVMIRSIFSQISEIANRISEAAQTMNEYAEDFQTSSENLKSTINESELRSSELN
                     QSTVDIVEVIHNQNTSLQKFKNDILNLNILIQSIEKEVREISKLSSVTKQEASGSKNK
                     IFELKSAMEDIQRNSESIQDITSLINDISEQINLLALNASIEAARAGAAGLGFRVVAN
                     EVSKLAENTASRVKEIGTLIKTTNKAIIRGIDVVNDAGLMMDNLMQSSEKVDELITRT
                     TGAIEQQSFKSNEAKLYIEAMALNSMQIENMIDEQKQKIHEMHIKIGSINSDSELIAE
                     GANKMNALAKDKVRTAKFLKQISGEFKIDNKYLLQWDSSFETGVNSIDSQHKELLNIL
                     NNLFTSVHMNHSTDKLAEILLGLVDYTVYHFNHEEELMRDCDYPQFAKHKEEHENLKK
                     AVLAFKKDFEAGTATISFELLDFLRRWLINHILYSDKKLGEFYTQK"
     gene            complement(5931..7007)
                     /locus_tag="PLF66_22115"
     CDS             complement(5931..7007)
                     /locus_tag="PLF66_22115"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HMZ66800.1"
                     /translation="MYTICVVDDEEISEKMLTLILRMEKYNVISASSGKKALKLLEEK
                     TPDLFLLDIDMPEMTGLELIAVIKTKPEFKEVPVIFISAHQDVDTKVEALKLGAIDFI
                     SKPFQNPEVITRVKVHLENSILHKKLKELNNKKNTFFSIIAKDIKDDLDEIYSRITSL
                     QDFINTNDLKAAKESLSSLNKFNTQTRYYLRNLIYWSRLETNLIRLQKDKVNISKIFS
                     DIIKDYKEPIEHKKITIENNLAESDRFILADEYSLKVTIDNLFSNAIKFTNYHEFVKI
                     LCNVEGSNLTISILNKGMFLSESIIKKILNFEYHMDRPLNESEKGSGLGLALSKELIE
                     KNEGKFWIQTNENITEIHFSLPMG"
     gene            complement(7098..8093)
                     /locus_tag="PLF66_22120"
     CDS             complement(7098..8093)
                     /locus_tag="PLF66_22120"
                     /inference="COORDINATES: protein motif:HMM:NF025598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HMZ66801.1"
                     /translation="MKSIFESYNKIFEFCMKNIALTLTAANIIFLLLILIVKDPFGLR
                     ISSYDQASPFLETKQTITKITIEKVKVADSSFELNKQSEEWILTAKGKTIPVDKDRIE
                     SLLKSIANARKYTIVSSSKDKSEEFGFNEDEIKIEMFSSNNSLGYFRLGSASTTDANS
                     SYIKWKDSDDIYLIEENLRASTNRADFTYFLNKRVSPIGLTSEDLIGVTLKKNGNNFE
                     IRKTNTWNLIAPKKGDLANEDMNTVLSKLSSVNAEDIVLDESTLKGIDPNPFELRVDY
                     KSKDGIAKSYTLLSAGFDKKLNAYYIRKNNEPLIYKLSEYSIKSILEFKPETLVK"
     gene            complement(8099..9775)
                     /locus_tag="PLF66_22125"
     CDS             complement(8099..9775)
                     /locus_tag="PLF66_22125"
                     /inference="COORDINATES: protein motif:HMM:NF021351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GldG family protein"
                     /protein_id="HMZ66802.1"
                     /translation="MKEYFSKLNKNYKFLSLSAFLSFFLFNYLMSDFYCRKDLSRENR
                     FNLTNSTEKVLQGLPEKLYIDAFYSSDVPGEHKARLNLTREMIKEIANVNRKKVELRF
                     HDPDANESDKKKAQEAGIKPAQLNQQERGSVEIKQAYFGIKLTVGSKSTVIPNAYFAE
                     AIEYQILSSLKKMLKKNNASTLAILKVPGAFLAPDQPQSMGKDTFGLFTHRAFAPENG
                     EVQEINVNAESIPDEIKTLLWVGSPNLTEKGKYYIDQFLMRGGNLLIFAKTMNFQLSE
                     QNQMAALMGGGNEGIAQQAPEAQAINAFLSHYGVEIKNDMVLEPDDSYAVTSIFQQLT
                     NPNAEAFHYPLWLIPRKESGSINTKSEFTKNASAVLLPWSSTLDLKPDKQPSAQFTTL
                     IESTNKADKRAEFILVNEEKVAKQPINPQNTKFPLAIHIEGNLKSFFTQDTIPKEVTE
                     AFIDKTKEGKKSQILVFGSPYIVSDMLATNDYLSLFRVNLSFVLNTIDILSGDTDMLA
                     LRTKQVYTKNLKDVNKTEKFIYSFINILLIPVGIGVFAFYRLKKRSSGKR"
     gene            complement(9772..10503)
                     /locus_tag="PLF66_22130"
     CDS             complement(9772..10503)
                     /locus_tag="PLF66_22130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016548299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HMZ66803.1"
                     /translation="MTARNIKTIFKKESATYFNTPIGYIFSAFFLLLISFLFFYGLGG
                     NSFWDLKIASLEQFFSWIPILYIIFIPAITMRIWSEEEKSGTLEVLMTLPIKDYEIVI
                     GKFLSAWMFLTVTILCTLLAPLTVRILGDLDFGLVFMGYIGTILLGGAYISIGLIISS
                     LTKDQISAFILTLLACFLMFIMGYQPILKFFGNFLGGIIAFLSLSQHFESFRMGVMDP
                     RDFLFFISFIKIILFLNVFVIRGKR"
     gene            complement(10500..11414)
                     /locus_tag="PLF66_22135"
     CDS             complement(10500..11414)
                     /locus_tag="PLF66_22135"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HMZ66804.1"
                     /translation="MLRVENLTRKFQDKVAVNDISFSVKPGVITGLLGPNGAGKTTTM
                     RLLTGYLEPSAGSILYNGKSFLEDTISVQKKLGYLPESAPLYQDMLISEYLNYMADIR
                     GVKEEDKEARIQKMIEVCELSSHLHTPISFLSKGFKQRVALAGTLIHDPEIIILDEPT
                     SGLDPNQISHIRTLIRGLGKEKTLILSTHILQEVEDICDEVIIINNGKIVANSSVKNL
                     HSGHTVSILVNADLSKVKSIFTEKPFLSVEEYSEINEGDFKGYLVHMEELKPELAFST
                     LAKSSLQVRELKTFKRSLESIFEELTRK"
     gene            11734..>11993
                     /locus_tag="PLF66_22140"
     CDS             11734..>11993
                     /locus_tag="PLF66_22140"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HMZ66805.1"
                     /translation="MTARATKPAKPKKEKNSFDLKEILLKIPMFSNLSHEALDLVIQI
                     CQTRKLKKNEILFKEGDAGDFAYIIEAGKIEIFTEISGDKVI"
BASE COUNT         4118 a         1790 c         2030 g         4055 t
ORIGIN      
        1 gtaagccatt aaactacttg actctctcat ttatatagaa aggctatcat tatgaaaaaa
       61 atctgttctt tatttctgat caccttttta ttacaagcag atagcaattt aaatatacga
      121 catgtaacga tagccgacat tgtttaccaa cctcataccg aggaaggata tttacaggga
      181 tggaatttct tttttagaaa ttcggaatac aatattattg ccacatttct agtaagtaat
      241 ttaggaccaa atgattttaa taatggagta agtattgcta ttgaatctaa aaagacaggt
      301 tctcttttta ttaccaaaga atttggacag aaggatttga atgtagagaa tgggattaat
      361 ttaaaaatgt ataataacac tttaaagtat gaaagtggca aatttgaatt atcccaatac
      421 tatgatgata ttaaaatatt tttaaagttc gaatcagatg gtttaggagc aactttatca
      481 ggtggaaaac attttgtgaa gggaaatgat aagtttgtaa gagcagatat tccattttca
      541 tttgttagag cgacagggta tttagacttc aaaggggaaa ttgtagagtt ggttggagta
      601 ggtggattag agcatttgat aacaaattat gaagtatata aatacagtag caaatgggaa
      661 atactgagag catcaaacaa agagggaatg aagctttaca ctggtggttt tcatggtata
      721 aagaaaaatg cagatgattt ttttagaacc attgtgatac agaatcctaa aggagaaatt
      781 attttgtcag gcattgtcac aaaatcggaa attatttctt cggagttaga taaattttca
      841 ggttatgtct tacccataaa ggaaaaaata tacattgggg ataatggatg ttatgtgcaa
      901 tcggagagac tttatagtgt aggtaagatt aatatacttt ctaatatatc tgccgttctt
      961 cggtttttta taaaattaat ttttgcaaac ccttaccaag ttaatttttt aacaaaacta
     1021 aatgtagttt gcaatgatag cttgaagata tctacatctg attttattgg aattcactcc
     1081 tattatctga ttaatcccaa gtaaaaaaag aaaaaataca ttcggatttc tgaattttaa
     1141 accacaaaag aaagtcgata gttccttatt cgcttaatct agatcgatta tcgtttaatt
     1201 ctgcaatcag gatttttgaa tattgataat gtatggtgta ttctagggtt tgattttatt
     1261 aaatatacac taaaaaactt gaccaatatt gcttaacata tttatctttc cttgaggaat
     1321 gctttgaaaa ttgatttagc tcattgcgaa agagagccta tccatatccc tgatttaatt
     1381 cagccccacg gatatgcaat tgtttatgaa aaagaatcgc tgatcataac tagatgcagt
     1441 gataatataa acttaattga aaaagattta tttataaagt ttttagataa gaggataacg
     1501 gatgttatcc cgtatccttt atttgaaaaa attcaaaaaa aaacaaactt tagtttgcat
     1561 aaaagagatt tattgctcaa tgaatctcta cccactttga atattcagaa tgcagcaatt
     1621 tttgtaagtg atgcatttga ggaagttata atagaaatca taccaacctt cgtatttacg
     1681 gatgattcgc ttttagatac tgaattaaat gacatgatag aaagactgat caaaagtcct
     1741 acaatttctt ctatgtttga agtcgctgtt aaagatatca aaaaattgct tggatacgat
     1801 cgggtgatgg tttatcagtt tgattccgag ttcaacggag aagttttatc agaagctaaa
     1861 gaagacggaa tggagtctta cctaaatttg cactatccag ctactgacat tcctgctcaa
     1921 gcaagagagt tatataaaac taatatgatt agaacgattg tggataatca ttatataccg
     1981 gtaaaattta tttcttccca aaaaaatcct ctgaatatga gtcatagcta tttaagaagt
     2041 gtatctccca ttcatttaga gtatctagaa aacatggggg ttagggcgac tcttacagtt
     2101 tctatcatgg tggaaggaaa gctttggggg ttgattagtt gtcatcacag agaaatcttt
     2161 tttcctgata tcaagaaaca aaacattgca caaatttttg gaagtattct tggtggaatt
     2221 atccaaatga gagaggaagg agaaagagag aagaagagta gcggactttt atctagactt
     2281 gatttagtca tggagatgct attattccaa aatactggaa gtgaactttt aactctcata
     2341 cgcagtaaaa tagttttatt acaatcaatc ttttcgtctg acggattcat ggtttttata
     2401 aatggaagta ttatcgcaca taattttcct ctttcagaaa gtcaaatttt agaattgatt
     2461 gaaaaattag atcctttttt aactgagaaa attttctaca ccgatagtct tacggaaaca
     2521 tttccaaatt taccagaaat agttttgaaa ctatgtgctg gattaatggt tgttaaatta
     2581 gattcaaaac ccagaagtta ctggatatgg agaagggtag aaaaaacgca aacgattgcc
     2641 tggggaggaa acccaaatca aaaagcaatt ttaaatcaaa agggagaaat ttctccacgt
     2701 aaatcttttg aaaaatacaa tcaaattgta actaaaaaat cagttccttg gaatcgttca
     2761 gaaaaagatt tctatattta tctaatccct agactacatc gtttgtatga aatatttgaa
     2821 tcaaaccaag agatagagtc acaaaagcgt catatactac atatcgaaga agaaagagct
     2881 aaacattttg aagaattaat tgaaatgtta gtagagctta tagaaatgag ggattcatat
     2941 acaggaaatc atactaaaag agttgcagac tactcagtcg ctattgcgca agaattaaaa
     3001 ataaaatcgg atgagattcg cttattaaga gaagcatcta tattacatga cattgggaaa
     3061 attataattc cagactcaat actacttaag cctggaaaac tatcgtataa agaatatgaa
     3121 ctgattaaac aacatgttat ggtaggtcac acaattctag ataaaataga ttattataaa
     3181 cctatcgcct atataatttt gcatcatcat gaaaagtttg atggttcagg atatcctttt
     3241 ggtaaaaaag gaagcgatat tccctatctc tcacatatta tgatggtagc tgattccttc
     3301 gatgcaatga ctacaaatcg aatttatcaa gcacgcaaat caaatgaaga ggcaagaaag
     3361 gaacttttaa aatacaaagg aatatggtat catccagatg tagttgatgc agcgatacgt
     3421 gttatacaaa aaaatgaaaa agaagattta aaaactactc aacttccaat tacgcaaata
     3481 gagcaagaaa gatttgcata tttcttcaaa gatcagctaa caggagttta caatgctact
     3541 tatctctgga tgggaatcaa cgatctgatt tctggtattt cttcagaaca ttttttactt
     3601 ttagagttac atgggatgtc cgatttaaat tctcaactcg gttggaacaa aggaaatgaa
     3661 gtgattcaag aatttgcggg atacctttta gagcaagtga aaggagataa agtctatcga
     3721 gtgtttggag atgattttat agttgcattt gatacaatcg aagaaaaaga aaagttttta
     3781 aaaaattgta aaacatttag aattgaaaat gttttctccg aatgtaaaaa catagaaaaa
     3841 gataaattta aagagatatt ataatcattc taaataattt ggggttaatt gcggttttcc
     3901 gcaatgaatt ttgaaaatag gtttataata ctcgagagac aaacaccagt tacctcttcg
     3961 taataatcta tttttaacag aaaagttttt ccatatttaa aaccacttgt attttactca
     4021 tagtctgata ataaagaatg gtaggtgcaa ttaaatgaat ataaaaaaaa taattaagac
     4081 cattcgtgaa agtaaaatac aggtgaggtt attaataacg tatatctttt ttttattagt
     4141 attaatgtca tttcccattt ttttatttta taacaattat caatctaaat ctctctctca
     4201 gaatcaattg caattaattg aagaccatat gaagaatctg gaacaatatg agaaagattt
     4261 aaatttaaat ctccaatatg agaaaagctt aaaaattaaa tcggaagaaa tacaaagaga
     4321 gacaaatgga catttaaata aaaacctttt ttatattcta atttttacaa ttattataat
     4381 catcggtagc tttataacag tgatggttat cttaaaagac tttattaaac cattaaagga
     4441 ggcaatatct gcagcagaaa gactttcaag taatgatatg aactttgaag taaacgatag
     4501 attcatggat gaaatgggat cacttttaca tcatattaaa attgttcgcg tgatgattcg
     4561 tagtatattc tcccagatca gtgaaatagc taatagaata tcggaagctg cacaaactat
     4621 gaatgaatat gcggaagatt ttcagactag ttctgaaaat ttaaaatcaa caataaatga
     4681 gtctgaattg agatcctctg aattaaatca atctacagtc gatattgtgg aagtcatcca
     4741 taatcaaaac acaagtctac aaaaatttaa aaatgatatt ttaaatctaa acatattgat
     4801 tcaaagtatt gaaaaagaag tgagggaaat ttccaagctt tcctctgtaa caaaacagga
     4861 agcgtctgga agtaaaaata aaatctttga attaaaatct gctatggaag acattcaaag
     4921 aaattccgaa tcaattcaag atatcacttc tttaatcaat gatatttctg aacaaataaa
     4981 tttactagcg cttaacgcat cgattgaagc agctagagca ggtgctgccg gattaggatt
     5041 tagggtagta gcaaatgaag tttctaagtt agcagaaaat acagcctcgc gtgttaaaga
     5101 gattggcact ttgattaaaa ctacaaacaa agctatcatt agagggatcg atgttgtaaa
     5161 tgatgcaggt ctcatgatgg acaatctaat gcagagttca gaaaaagtag atgaattaat
     5221 cactagaact actggcgcaa tagagcaaca aagctttaaa tcaaatgaag ctaaattata
     5281 cattgaagct atggctttaa attcgatgca aattgaaaat atgattgatg agcaaaaaca
     5341 aaaaatccat gaaatgcata ttaaaatagg ttccattaat agcgatagtg aattgattgc
     5401 ggagggagca aataaaatga atgccctcgc aaaagataaa gtaagaactg caaaatttct
     5461 aaagcaaatc tcaggtgaat ttaaaattga taataaatat ttactccagt gggattctag
     5521 ttttgagaca ggtgttaact ctatagattc acaacataaa gaattactta atattttaaa
     5581 taatcttttt acatcggtgc atatgaatca ttctacggat aagttagccg aaatactatt
     5641 aggtcttgta gattatacgg tatatcattt taatcatgaa gaggaattaa tgagagattg
     5701 cgactatccg cagtttgcaa aacacaagga agaacatgag aacttaaaaa aagctgtcct
     5761 tgcatttaaa aaagattttg aggcagggac ggcaacaatt agttttgagc tattagattt
     5821 tctcagaaga tggttgatca atcatatttt gtattcagat aaaaagcttg gtgagttcta
     5881 cactcaaaaa tgaaaaaatc ttcttaattc agtttagtat taaaaataga tcatcccatt
     5941 ggaagactaa aatgaatttc tgttatattt tcatttgttt gaatccaaaa ctttccttca
     6001 ttcttttcta tcaattcttt gctaagagct agacctaatc cagaaccttt ttcagattcg
     6061 tttaacggtc tatccatatg atattcaaag ttaagaattt ttttgattat agattctgat
     6121 aaaaacattc ctttattcaa gatagatatt gtaagattcg aaccttccac attgcataga
     6181 atttttacaa attcatgata gttagtaaat ttaatcgcgt tggagaataa attgtcaatt
     6241 gtcactttca aggaatactc atctgctaat ataaatcgat ctgattctgc taaattattt
     6301 tctattgtaa tttttttatg ctcaatgggt tctttataat ctttgatgat atcactaaag
     6361 atctttgaga tatttacttt gtctttttgt aatcgaatta aatttgtctc tagtctcgac
     6421 cagtaaatta aatttcgtag gtaatatcta gtttgcgtat tgaatttgtt tagacttgat
     6481 agagattctt ttgctgcttt caaatcattt gtgttgataa agtcttggag ggaagtgatt
     6541 ctagaataaa tttcatctaa atcatctttt atatcctttg cgataattga aaaaaaggta
     6601 tttttcttgt tatttaactc ttttaatttt ttgtgcagaa tgctattttc taagtgaact
     6661 tttacccgtg taatcacttc gggattttga aaaggttttg aaatgaagtc aattgctcct
     6721 agttttaatg cttcgacttt tgtatctacg tcttggtgag cacttatgaa tataactggc
     6781 acttctttga attcaggctt tgttttaata actgcgatta gttcgagtcc agtcatctca
     6841 ggcatgtcta tatctagaag aaataaatct ggagtttttt cttctaaaag ttttaaagcc
     6901 tttttaccac ttgaggcaga aataacgtta tatttctcca ttcttagaat gagagttagc
     6961 attttttcac ttatttcttc atcatccaca acgcaaatag tatacatctc catccttcct
     7021 acaaggaaat gtttagttat ctaatgattt tgtcaacgga taaaaaaaca ccctacaact
     7081 agcagggtgc ttctttttta ttttacgagt gtttctggtt taaattctag aattgatttg
     7141 atagaatatt cacttagttt ataaataagt ggttcattat tttttcgaat ataataggcg
     7201 tttagttttt tatcaaatcc agcggataac aaagtgtaac tcttcgcaat tccgtctttt
     7261 gatttgtaat ctactctcag ttcaaatgga tttggatcaa taccttttaa tgttgattca
     7321 tccaaaacta tatcttctgc atttacagac gaaagtttag aaagaactgt attcatatcc
     7381 tcattcgcaa gatctccttt tttaggagca attagattcc aagtatttgt ttttctgatt
     7441 tcaaaattgt ttccgttttt ctttagagtg actccaatta agtcttcaga cgttagtcca
     7501 attggagaaa ctcttttgtt taagaaataa gtaaaatcag ctcgatttgt actcgctctc
     7561 agattttctt cgattagata aatatcatct gaatctttcc atttgatata agagctattt
     7621 gcatccgttg tagaagcaga acctaaacga aaatagccga gtgaattgtt ggaagaaaac
     7681 atttctattt taatttcatc ttcattaaaa ccaaattctt ccgacttatc tttagaagag
     7741 cttacaattg tatacttacg agcatttgcg atagacttta gaagagattc gattctatcc
     7801 ttatcaacag gaattgtttt tccttttgcg gttaatatcc attcttcact ttgtttgttt
     7861 aattcaaaag aagaatcagc tacttttacc ttctcgattg taattttagt aatagtttgc
     7921 tttgtttcaa gaaaaggact tgcttggtca tagctactaa ttcttaatcc aaatggatct
     7981 ttcacgatga gtataagtaa aagaaaaata atattagccg ctgtgagcgt cagtgctata
     8041 tttttcatgc agaattcaaa gattttatta taagattcaa aaatagattt catagaaatt
     8101 accttttacc agaagatctt tttttaagac gatagaatgc aaatactcca atcccaactg
     8161 ggattagaag tatattaata aaactataga taaacttttc tgttttattt acatccttta
     8221 ggttttttgt ataaacttgt ttggttctga gggctaacat atctgtatct ccactcagta
     8281 tatcaatcgt atttaataca aagcttagat ttactctaaa aagagataaa tagtcgttag
     8341 ttgctagcat atcagataca atataagggc taccaaatac tagtatctga ctttttttgc
     8401 cttctttggt tttatcaatg aaagcttctg taacttcttt tggaattgta tcttgtgtaa
     8461 aaaaagattt taaattgccc tcgatatgaa tagccagagg aaattttgtg ttttgaggat
     8521 tgattggttg ctttgctact ttttcttcat ttacaagaat gaattctgct ctcttatctg
     8581 ctttgtttgt gctttcaatg agagttgtga attgagcgga aggttgtttg tctggtttta
     8641 aatcgagagt agaagaccaa ggaagcaaaa cagcacttgc attctttgta aattcactct
     8701 ttgtattgat acttccactt tcttttctag gaatgagcca gagtggatag tgaaaggctt
     8761 ccgcatttgg gtttgtcaat tgttgaaaga ttgatgtaac cgcataagaa tcatcgggtt
     8821 ctaaaaccat atcatttttg atttcaactc cataatgaga taaaaatgcg ttaatcgctt
     8881 gagcttctgg agcctgttgt gcaattcctt catttccgcc tcccatgaga gctgccatct
     8941 ggttttgttc tgagagttga aaattcatag tctttgcaaa gataagaaga tttccccctc
     9001 tcattaaaaa ctgatcgata tagtatttac ctttttctgt taaattagga gaacctaccc
     9061 agagaagagt tttaatctcg tcaggaatac tctctgcgtt cacattgatt tcttgcactt
     9121 ccccattttc aggcgcaaaa gcacgatgag taaaaagccc aaaagtatct tttcccatac
     9181 tctgcggttg atctggagca agaaaagctc ctggaacttt tagaattgca agagtggaag
     9241 cattgttctt ttttaacatt tttttaaggg aagaaagaat ttggtattca attgcttccg
     9301 caaaataagc gttagggatt acagtggact tagaaccgac agtgagcttg attccgaaat
     9361 aggcttgttt gatttctaca gaacctcgct cttgttgatt gagctgcgct ggtttaattc
     9421 ctgcttcttg tgcttttttc ttatcagatt catttgcatc aggatcatga aatctgagtt
     9481 caactttttt tcgatttaca tttgcaattt ctttgatcat ctctcgtgtg aggtttaatc
     9541 ttgctttgtg ttcccccgga acgtcagacg aataaaaagc atctatgtat aatttttctg
     9601 gaagaccttg taaaactttt tctgtgctgt ttgtcagatt gaatctattt tctctcgata
     9661 gatcctttct gcaatagaag tcgctcatca aataattaaa taggaaaaaa cttaaaaacg
     9721 cactgaggga aaggaattta taatttttat ttaatttaga aaaatattct ttcatctttt
     9781 acctcgaata acaaatacat ttagaaataa aatgatctta ataaaactaa taaagaacaa
     9841 gaaatctctt ggatccatga ctcccattcg aaaggattcg aaatgttgag acaaagaaag
     9901 aaaggcgatg attccaccta ggaaatttcc aaaaaatttc aagataggtt ggtatcccat
     9961 gataaacatc agaaaacaag caagaagcgt tagtataaat gctgagatct gatcctttgt
    10021 aagagaagaa atgatcagac caatgctaat ataggctcct cctagtaaaa tcgttccgat
    10081 gtaacccata aagaccaaac caaaatctag gtcaccgagg atacgaactg ttaggggagc
    10141 taggagtgtg caaaggattg ttacagttaa aaacatccag gcagataaaa attttccaat
    10201 tacaatttcg tagtctttga taggaagagt cattagaact tctaaggttc cagacttttc
    10261 ttcctccgac cagattcgca ttgtaatggc tggaataaag atgatgtaaa gaattggaat
    10321 ccaagaaaag aattgttcca gacttgcaat ttttaaatcc caaaaggaat ttccaccgag
    10381 accataaaaa aataaaaaag aaattaaaag taaaaagaat gcgctaaaaa tataaccgat
    10441 aggcgtatta aaataagtag cagattcttt tttgaatata gttttaatat ttcgggctgt
    10501 cattttctag ttaactcctc aaagatagat tctaatgagc gtttgaatgt ttttagttct
    10561 ctcacttgca gggaagattt tgcaagagtg gaaaatgcta actctggttt caattcttcc
    10621 atatgaacta aatatccttt aaaatctccc tcgtttattt ctgaatattc ttccacagaa
    10681 agaaaaggtt tttctgtaaa tatagatttt actttggata ggtctgcatt cacaagaatc
    10741 gatactgtat gtccagaatg tagatttttt acagaagagt ttgcgactat ctttccgttg
    10801 ttgatgataa tcacttcatc acaaatatct tctacttctt gtagaatatg ggttgatagg
    10861 ataagagttt tttcttttcc aagtccacga attaaagttc taatatgaga aatctggttt
    10921 ggatcgagtc cagaggttgg ctcatccaaa ataatgattt ctggatcgtg aatgagtgtt
    10981 cccgcaagag ctactctttg tttaaatcct tttgataaaa aactaatcgg agtatgtaga
    11041 tgagaagaaa gttcgcaaac ttcaatcatc ttttgaatgc gtgcttcttt atcctcttct
    11101 ttaactcctc gaatatctgc catgtaattt aaatattctg aaatcaacat gtcttgatag
    11161 agtggagcag attctggaag gtatcctaat ttcttttgaa cagaaattgt atcttctaaa
    11221 aaagatttac cattgtataa aatagaacct gcacttggtt ctaagtatcc agttaggagg
    11281 cgcatcgtag tagtttttcc cgccccgttt ggacctagaa gtccagtgat gacaccgggc
    11341 ttcacagaaa atgagatgtc gtttacagct actttgtctt gaaattttct agttaaattt
    11401 tctacacgta acatttgtat tcaccttgat tacaaaattt tgaaaaacct ttcagtgtcg
    11461 atactttttt cccctagaaa acaatgcttg tggctaatgt tgattatccg taaatagact
    11521 ctaaatcctt tttaaaaaat ccaatttttt tggtctagtt gtgtctacgc atcctttctt
    11581 ctagttataa gaataggatg ccaaatggaa tttatttagt gggaaactat aagcttttag
    11641 ttataaaacc atttatttcc gagaatcgta ttcaatattc gattgactct tatcctataa
    11701 tttattgcga tagcagtagt gcctatatca cccatgacag ccagagcaac aaagccagcc
    11761 aaaccgaaga aagagaagaa ttcttttgat ttaaaagaga ttttacttaa aattcctatg
    11821 ttttcaaatc tctctcacga agcattggat ttggtcattc aaatttgcca aacccgtaag
    11881 ctgaaaaaaa atgaaattct attcaaagag ggggatgcag gggattttgc ttacatcatt
    11941 gaagcgggga aaattgaaat ttttacagaa atttcaggtg ataaagtcat att
//
LOCUS       DAOQOS010000145        11635 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_2856_length_11635_cov_6.701727, whole genome shotgun sequence.
ACCESSION   DAOQOS010000145 DAOQOS010000000
VERSION     DAOQOS010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 11635)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11635)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11635
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2856_length_11635_cov_6.701727"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..209)
                     /locus_tag="PLF66_22145"
     CDS             complement(<1..209)
                     /locus_tag="PLF66_22145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66806.1"
                     /translation="MIKLPVKYTFYFKKNRQFLLGLIIIVLQLAINLSISSEPKSKRL
                     RYQKNLKRVEEFKKNMSEPEKAYYLP"
     gene            343..777
                     /locus_tag="PLF66_22150"
     CDS             343..777
                     /locus_tag="PLF66_22150"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HMZ66807.1"
                     /translation="MKKILAILAISLTSTLTADSEINSVENILKNSRPADLPAIAGNY
                     SALGSTHQIKGDYSSAMSNYKKSLQIRQAIGLDRTQGYATVLFLLSIVEHKMGESCKA
                     AENIKKVIKIYNYLGMVDDANVAEKEGLKEFKSACSELVSQN"
     gene            complement(774..1562)
                     /locus_tag="PLF66_22155"
     CDS             complement(774..1562)
                     /locus_tag="PLF66_22155"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HMZ66808.1"
                     /translation="MNQFLNVVGVQLDIVWEDKEANFQKIRDILSRLDSKPDLIVFPE
                     TFATGFTMRSLDFAEAQLGPTEKFLIEMAKKTGSVVGGSWMEENKDGMPFNTFSIARP
                     SGIITNRYHKIHPFSFAEEDKFFTAGEKTETFELNGFHISLLICYDLRFPEVFRKTAG
                     STDLYLVIGNWPEARIDHWLSLLKARAIENLSYVLGVNRVGYAGRKNQLYHTGHTAMF
                     DPFGVGEILESKKEEILKYRISSRVLKEFREKYPFLKDRRKSLF"
     gene            complement(1559..2557)
                     /locus_tag="PLF66_22160"
     CDS             complement(1559..2557)
                     /locus_tag="PLF66_22160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000846602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66809.1"
                     /translation="MHNRLVFLFLFLFITGLNAEELNLKISLKNGTTGGVGQADSIKL
                     IALQGGMVPVGEFSNKSGNFVLEKVNVPDGSPILIQVSYKGANYNKMVPPAPNFRNKV
                     QEISVYETTSDRKNIEVRSLLQIVREENLVRLYKIFLIENKSNPPRSFQDKNNPLEVF
                     VPAEATEVFGQLTQGDSKMGIPLNLVDGKKGKMIDRSILPGSSELQITFVLSAKSLGD
                     ISFKDELNFEDPKSPRIIFYKPKDMKVELKGSMSTEEIKENIPEGLGAIRAIYSETNS
                     VEISVAGGNFEPEEPRTNSPKVRNGSIFTTWDKSVFAVIGFLGIFFSLSFLFVYRK"
     gene            3108..3230
                     /locus_tag="PLF66_22165"
     CDS             3108..3230
                     /locus_tag="PLF66_22165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66810.1"
                     /translation="MIKYFLTSFLIALSIASVSLSACDGKGKLVEDKPIEATTK"
     gene            complement(3270..5117)
                     /locus_tag="PLF66_22170"
     CDS             complement(3270..5117)
                     /locus_tag="PLF66_22170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66811.1"
                     /translation="MYSKKYSVSLNRKIVSIYISLAIGIYALYKAYILHWVCDDAFIS
                     FRYAKNLNDGLGLVYNAGEFVEGFTNFLWTLFISVGMFFQLDPIHVSTGLGLFFYLLS
                     LFFLYKTGNTIFKIRAGEHFKSFYFPVAFIGYALHSHGQIFATGGLETTFFGFLLLAG
                     SVFILLSDRGLHVLIGLFLLVLSIMTRPDGALFYIFATLYVLLFKYRNLEYRTYFINQ
                     FVLQLPFLFIFLPYWFWRYTYYGWFFPNTFYAKSGMESYLEQGIKYTYLYFSSYYILV
                     LPFLLVLFWFLRITRVSSKLRYLALVLFSRRYKMRRRKLRLRKRNLYRIKPSFSLKRM
                     ISGQSRIDHFYIRVFLLLFLPSFLYILYLTKIGGDFMFARLLIPITPLLYLTTELFLF
                     RMKFPRVRNVLSLMVVICTLFYNNPYKGSQYPIVDNITNESDVYKLKSVYELKLSLLP
                     FTKIFEELEVSIAFGGSQAMIAFYLNPRIAIESVTGLTDEFIAHQTIYERGKVGHEKA
                     APIDYLLKRNINIHFFPTNEIPKTDYNWIRLKNVPGEFRILRYDAILFSELKKTGKFE
                     FVNFESYLDRYISEIDRNSPAQIQKDYKHFYKYYFEINQDDVRERYFLE"
     gene            5176..6354
                     /locus_tag="PLF66_22175"
     CDS             5176..6354
                     /locus_tag="PLF66_22175"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HMZ66812.1"
                     /translation="MKKKIALISPIFSPNISGGAERHAISFAELLSEDYDLEILTTKA
                     LDYITWKNVIKEDTETFHASLVRRFKVKETRNIKSFNRYHARLIHKLPNISEKEMEKW
                     IQKQGPYCPDLIHFIRENQHKYDVFIFMTYLYYTTIYGMSEIKNKSICVTTLHDEPPA
                     YFPIYKKILTNEISYSFNTPEERDLFVKIFSYKPKHYSIIGLHVDQPAIVHLPKPKGN
                     YVVYIGRIDAGKGIYTLIEKFLEWRNKYNEEIDLVLIGGGEQVLASEHVRFTGYISEE
                     EKINYLKNALFLINPSHLESFSIVVMEAWLLEIPVLVNAESEILKAHCIRSNAGLYYS
                     DQENFEATFNYLLDHPETRKKMGRNGRLYVEMNYTRDKVKQKLLSLVESIKKRDSAKT
                     "
     gene            complement(6755..8428)
                     /locus_tag="PLF66_22180"
     CDS             complement(6755..8428)
                     /locus_tag="PLF66_22180"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016544796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HMZ66813.1"
                     /translation="MEPFDTSFIAQIASKLYNELPKANSISGSSFKVAANKYMPEKSF
                     SGKEKDLDFEKIQEIVFGKNTSEITDRNESVSEKLNLDQKTISESVGTLKKQQSEIVG
                     ERKYDNLKSFVESIQGKNSNHNLNWNGLDNCVFFQKQSDSRFVKDIYSNQPFDVNQIR
                     KDFPVLNQKVHGKPLAWFDNAATTQKPKIVIDAISHYYSNDNSNIHRGAHTLAARSTD
                     AFEDAREKVKNFIKASSTSEIIFVRGTTEAINLVAQTYGRKYFQAGDEIILSQAEHHA
                     NIVPWQIVAKEKGAIIRVIPLNERGEVILEDYERLLGPRTKFVSLTQASNSLGTILPV
                     KEMIGLAKRHGAKVLIDGAQSVAHIPVNVQDLEADFFVFSGHKIFGPTGIGVVYGRKE
                     ILELMPPWQGGGNMIKNVTFEETTYSDLPAKFEAGTPNIADAVGLGAALDYVNSIGLE
                     NIAKYEHSLLEYATAGLTQIKGLRLIGTAPNKVGVLSFVLQNKQTSEVGRLLDLEGIA
                     VRAGHHCAQPSLRRYGVESTVRPSLSFYNTKEEIDRLVDAVRRIAQNHF"
     gene            complement(8442..9881)
                     /locus_tag="PLF66_22185"
     CDS             complement(8442..9881)
                     /locus_tag="PLF66_22185"
                     /inference="COORDINATES: protein motif:HMM:NF041163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0140737 - encapsulin nanocompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 2B encapsulin nanocompartment shell
                     protein"
                     /protein_id="HMZ66814.1"
                     /translation="MSDNILQRSVTTSVARNLANTTKTRPQMMSITPRYLLSLLPWVH
                     VEGGTYRVNRTKVELKKAERVELNFKDGAVSFNPQSLRSVPLFAKFQDDIINRMANRF
                     KTEEVTLGNKLVVEGVDKNKFFIIAQGQVEVLSKGVHGSDLRIALLTEGEYFGEIELV
                     SDIPSDVTVRTITPCKLLTLSGKDLDDILSEFPNLKEDFKKAIDKHIELRSTVNKYGE
                     RNIDLVSGFAENVEIPETFIDYSDKPREYSLNAIQTVVRVHTRVSDLYNNPYNQLEEQ
                     MRLTIEGIKERQEWELINSKKFGLLHSVDPSMRISTRYGAPTPDDLDELLALVWKKPA
                     FFLAHPKAIAAFERECTWRGVPPVTTQIFGSPVITWRGIPIIPCDKLEVKNTNLSNQW
                     FGTTSILLLRVGEEEQGVVGLHQIGIPGEISPSLSARLMGLDNLGVASYLLTLYSSAV
                     VLTDDALAVLENVEVGYYHDYQNRNSLPK"
     gene            complement(9898..11361)
                     /locus_tag="PLF66_22190"
     CDS             complement(9898..11361)
                     /locus_tag="PLF66_22190"
                     /inference="COORDINATES: protein motif:HMM:NF041163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0140737 - encapsulin nanocompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 2B encapsulin nanocompartment shell
                     protein"
                     /protein_id="HMZ66815.1"
                     /translation="MSDNILQRSVTTSVAKNLTNTTKTHPQMMSITPRWLLSVIPWVQ
                     VEGGTYRVNRTRVELKRAERIELNIVDGFVFLSFNPEALRTVPVFSKLPLDILTRISN
                     QFKTEEVSLGTTLVVEGEDKNKFFIIAQGQVEVFSKGVHGSDLRIALLTEGEYFGEQG
                     LLSDKPSDVTVKTTTPCKLLTLYGADLEQILNEFPNLRNDFRKAIDDYIELLSTVNKY
                     GERNIDFVSGFAENVEIPETFIDYSESPREYSLNAIQTVVRVHTRVSDLYNNPYNQLE
                     EQMRLTIEGIKERQEWELINNKKFGLLHSVSPAFRISTRYGAPTPDDLDELLALVWKR
                     PSFFLAHPKAIAAFERECTWRGVPPVTENSFGAPLILWRGIPIIPSDKLEVKSNYYSR
                     PWLGTTNILLVRVGEENQGVIGLHQAGIPGEILPSLSARLMGLDPLGVASYLLTLYSA
                     AAVLTDDAIAVLENVEVGYYHSYQNKNSNYFSDGLGI"
     gene            complement(11395..>11635)
                     /locus_tag="PLF66_22195"
     CDS             complement(11395..>11635)
                     /locus_tag="PLF66_22195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016544928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66816.1"
                     /translation="GAKKFSLDESGNPIKNEPRHPIIEDNVVIYSNANILGRITIGKN
                     STIGGNVWITTDIPPNSKITQAKVIQEHFYFGSGI"
BASE COUNT         3862 a         2220 c         1808 g         3745 t
ORIGIN      
        1 ggaaggtaat aagccttttc cggctcactc atgttctttt tgaactcttc cactcttttt
       61 aaattctttt gataccgtaa tctttttgat tttggctccg aggatatgct taaatttata
      121 gcaagttgta gaactataat aataagtcca agcaggaatt gtctattttt tttaaaataa
      181 aatgtatatt ttacaggcaa tttaatcata ctcccactca aaccaataaa aagtcgccga
      241 aattgaatta tttttactat tttttaatat cggtcagtag tttcgaatct ggtaccgaat
      301 ttgcatcctt atagatataa gattccaaaa ggagctacta ccatgaaaaa aatcctagca
      361 atcctcgcaa taagccttac aagtacctta acagctgact cagaaattaa ttctgttgaa
      421 aacattttaa aaaatagtag acctgcggat cttcctgcaa tagccggaaa ttacagtgca
      481 cttggaagca cccatcaaat caagggagat tattccagcg caatgagtaa ttataaaaaa
      541 tcccttcaaa tcagacaagc aataggctta gatagaacac aaggatatgc aactgtctta
      601 tttttacttt ctattgtaga acacaagatg ggtgaatcct gcaaagcagc agagaacatt
      661 aaaaaagtaa ttaaaatata taactaccta ggtatggtag atgatgcaaa cgtagcagaa
      721 aaagaaggtt taaaagaatt taaatctgct tgctctgaat tggtatctca aaactaaaaa
      781 agactttttc tcctatcctt tagaaatgga tatttctctc taaattcctt cagcaccctt
      841 gaggaaattc ggtattttag aatttcctct ttctttgatt ctagaatctc ccctactcca
      901 aatggatcaa acattgcagt gtgtcccgta tgatagagtt gattttttcg acccgcataa
      961 ccaacccgat tcactcctaa tacataagag agattttcaa tggctctagc tttcagtaaa
     1021 ctgagccaat gatcaattct tgcttctggc caatttccga ttactaaata taaatcagtg
     1081 gagcccgctg tttttcgaaa cacttccggg aaccgtagat catagcaaat cagtaagcta
     1141 atatgaaagc catttaactc gaaggtctcg gttttctctc ctgcggtaaa aaacttatct
     1201 tcctctgcaa aggaaaatgg atgaattttg tggtatcgat tggtaatgat tcctgagggt
     1261 cttgcaatag aaaaagtatt aaatggcatc ccatccttgt tttcctccat ccaacttccg
     1321 ccgacaacgg aaccagtttt ttttgccatt tcaattaaaa acttttctgt aggacctaat
     1381 tgtgcctcag caaaatctaa acttctcatt gtaaatccag ttgcaaaggt ttcaggaaat
     1441 acaataagat caggtttgga atctaatcga gagagtatat ctcgaatctt ttggaaattc
     1501 gcctctttat cttcccagac tatatccagc tgaactccga caacgttcaa aaattgattc
     1561 attttctata tacaaataaa aaggagagac taaagaaaat gccaaggaac ccaatcactg
     1621 caaatacgga tttatcccaa gtagtaaaaa tacttccatt tcgaactttt ggcgaatttg
     1681 tcctgggttc ctctggctca aagtttcctc cagcaacaga aatttctaca gaattcgttt
     1741 ctgaataaat tgctcgaatc gcacctaaac cttccggaat attttctttt atttcttcag
     1801 tggacatact tccttttagt tccactttca tatctttcgg tttataaaat ataattcgag
     1861 gggacttggg atcttcaaaa ttaagctcat ctttaaaaga aatatctcca agagacttag
     1921 ccgatagcac aaatgtaatc tggagttccg acgaaccagg taggatagaa cggtctatca
     1981 tctttccttt ttttccatct actaaattca gggggattcc catttttgaa tccccttgtg
     2041 tcaattgacc aaagacttca gttgcctctg caggcacaaa aacttccaaa ggattatttt
     2101 tatcctgaaa agatcttggt ggattcgatt tgttttctat tagaaaaatt ttataaagac
     2161 gaactagatt ttcctctcta acaatttgca aaagacttcg aacttcaata ttcttccgat
     2221 ctgatgtagt ttcgtagaca gaaatttcct gcactttgtt tctaaaattt ggagcgggtg
     2281 gaaccatctt attataattt gcacctttgt aagaaacttg gattaaaatg ggagaaccgt
     2341 ctggaacatt tactttttcc agaacaaaat ttccagactt attactaaat tcaccgacag
     2401 gaaccatccc tccctgtagt gcaattaact ttatactgtc tgcttgtcca actccacccg
     2461 tggttccatt ttttaagctg atctttagat tcagctcttc tgcgttgagt cccgtaataa
     2521 aaagaaatag aaataaaaat accaatcgat tgtgcataga ttcatatttt ttaatggaat
     2581 gatttgttaa aggaaaatat atactaattt gtcagaacaa attaaaaact cttttaaatg
     2641 gcactttctt cttttttatt tagaataaaa atgtagcgat ttactaaaac cttccttttg
     2701 attccaacga gtaccaagaa aaactccaga caaatcactt agaatcaaat aaaaaaattc
     2761 ggtatttttt attgatcaca tctatgaaaa caattaatac aaagacaatc aattatgaat
     2821 ctctttcatt cctcttattt tagtaagact tccttttttt tgttagataa tgatttaact
     2881 tgcggctaac aaaaagtagc cacaaaatta taaaaaaata tttttttcta aaaaaaagat
     2941 ttgctatttt aaaaaaataa gttatattat agacaatcga agagaaaggt ctgagggttc
     3001 aagtcgaaga ggtaaatgaa atactcaaga acgacaaagc cataaggcaa tatccggtat
     3061 aagacatcaa tctttggttg aaagttataa catgaaagga gtgtagtttg atcaagtatt
     3121 tcctcacaag ttttctcatt gccctttcaa tagcaagtgt aagtctatct gcctgcgatg
     3181 gaaaaggcaa attagtagaa gataagccaa tagaggcaac tactaaatag tttcacttga
     3241 ataatcatag aggtgcaatg cttctatgat tattccaaga aatatctctc tcgcacatcg
     3301 tcctgattta tctcaaaata atatttataa aaatgtttat agtctttttg aatttgcgcc
     3361 ggtgaattcc tatctatttc actaatgtag cgatctaaat acgattcaaa attgacaaac
     3421 tcaaactttc ctgttttctt caactccgaa aaaagaatcg catcataacg aaggattctg
     3481 aattcacctg gaacattttt taaacgaatc caattgtaat ctgtttttgg tatttcattc
     3541 gttggaaaaa aatgaatgtt tatatttctt tttagtaaat agtctattgg tgctgctttt
     3601 tcatgaccaa ctttgcctct ctcataaatt gtttgatgtg ctataaactc gtccgtcaaa
     3661 ccagtaaccg attcaatggc aattctagga tttaaataaa aagcaatcat tgcttgagat
     3721 ccaccaaagg caatacttac ttctagttct tcaaagattt tagtaaaagg taagagtgat
     3781 aattttaatt catagacact tttcaattta taaacatcac tctcatttgt gatattatca
     3841 acaattggat attgagatcc cttataagga ttgttgtaaa acagtgtgca gataacaacc
     3901 attagactca aaacatttcg aactcttgga aatttcattc taaataaaaa gagttctgtt
     3961 gtaagataaa gcaatggagt aattggaatg agaagtcttg caaacataaa atctcctcca
     4021 atttttgtaa gatacaatat atacaaaaaa gaaggaagga aaagcaaaag aaaaactcga
     4081 atataaaaat gatctattcg actctgccct gagatcattc tcttcaaaga aaatgatggt
     4141 ttaattcgat agagatttct ctttcttaat cttaattttc tacgtctcat tttatagcga
     4201 cgagagaaaa gaacgagagc caaataacgc aatttagagc taactcttgt tatacgcaaa
     4261 aaccaaaaaa gaactaacaa aaaaggtaag accaagatat agtaagaaga gaaatacaaa
     4321 taggtatact tgattccttg ctctaaataa gattccattc cagatttagc atagaatgta
     4381 tttggaaaaa accaaccgta ataagtatac ctccaaaacc aatagggtaa gaaaataaaa
     4441 agaaacggta gttgtaatac aaattgattg ataaagtaag ttctatattc caaatttcga
     4501 tacttaaaaa gtaaaacata aagagttgca aatatataaa aaagagcccc atcgggacga
     4561 gtcataatag ataaaaccaa cagaaaaaga cctattaaca catgtaaacc acgatcactt
     4621 aaaagaataa agacacttcc tgctagtaac aaaaaaccaa aaaaagtcgt ttcaagtcct
     4681 ccagttgcaa aaatttgtcc atgggaatgc aaggcatatc caataaaagc gactggaaag
     4741 taaaaggatt taaaatgttc ccctgcacga attttaaaaa tagtgtttcc tgttttatac
     4801 aaaaagaaaa gactaaggag atagaaaaat aaacccaagc cagttgatac atgaattgga
     4861 tctagttgaa aaaacattcc aacagaaata aataaagtcc acagaaagtt tgtaaatcct
     4921 tctacaaatt caccagcgtt gtaaactaaa cccaatccat catttaaatt ttttgcatat
     4981 cgaaaggaga taaatgcatc atcacatacc caatgcaaaa tatatgcttt atataatgca
     5041 tatatcccta tcgctaggct aatataaata gatacaatct ttcggttaag gctgactgaa
     5101 tattttttag aatacaaaaa tcgactccga tatttatctt tattttaaaa tgaatctggt
     5161 caatcttgat ttagcatgaa gaaaaagata gctcttatat cacccatttt ttcgcccaat
     5221 atatccggtg gtgcggaaag gcatgcgatt agctttgcgg aactgttatc agaagactac
     5281 gatctagaaa tacttacaac aaaagcactc gattatatta cctggaaaaa tgtaataaaa
     5341 gaggatacgg aaacatttca tgcaagcctt gtgagaagat tcaaggtaaa agaaactaga
     5401 aatattaaat ctttcaatcg ttaccatgca aggctaattc ataaacttcc aaatatctct
     5461 gagaaagaaa tggaaaaatg gattcaaaag cagggtcctt attgtcctga tctaattcat
     5521 ttcatacgag aaaaccaaca taaatatgat gtgtttattt ttatgactta tctctattat
     5581 acaacgatat acggtatgtc tgaaattaaa aataaatcta tttgtgtcac aacattgcat
     5641 gacgagcctc ctgcttactt tcctatctac aaaaaaattc tcacaaatga aatcagttac
     5701 tcatttaata ctccagaaga gagagattta tttgttaaaa ttttctctta caaaccaaag
     5761 cattactcca taatcggttt acatgtagat caacctgcta ttgttcattt gcccaagccc
     5821 aaaggaaatt atgtagttta tattggaaga attgatgcag gcaaaggaat atacacgtta
     5881 attgaaaagt ttttggaatg gagaaataaa tacaatgaag agatagactt agttttaatt
     5941 ggagggggtg aacaagtatt agcctctgaa catgtaagat ttacaggcta tatctcggaa
     6001 gaagaaaaaa tcaattattt aaagaatgca ttgtttttaa tcaacccatc tcatttagaa
     6061 agtttctcca ttgtagttat ggaagcatgg cttcttgaaa ttccagtatt ggtaaatgcg
     6121 gaatcggaaa ttttaaaagc ccactgtatt cgaagcaatg caggtttata ctactcagac
     6181 caagaaaact ttgaggctac ttttaattac ctactcgatc accccgaaac ccgaaaaaaa
     6241 atgggcagga atggcagact ctatgtggaa atgaattata ccagagataa agtaaaacaa
     6301 aaattactta gcttagtaga gagtattaaa aaaagggaca gtgcaaaaac ttagcatttt
     6361 tgaaaaagta tataaataac tcacatttcg caaaatttat tatttgagaa gtaaatgatg
     6421 attttaagat attagagaat gctaatgaat taataaaacc aatagcctgc gatagctcgg
     6481 ctgatggaga gcttataaag cgatggatgg acaaaccctc cgttccctct ctctcgcaca
     6541 gctcaagcct attgtcattt ttcaatgaca ataggagcaa acctcatttg aacgcttggt
     6601 tcacccgaac tcaatcattg cttttatgaa gcaatgataa tagctatagc gtaaggtgag
     6661 taaataaatc aaatttctct ataaatctat aaatctctct ctttaactaa gattttgcac
     6721 agatctactg aaattctaat gcatgaggta tatttcaaaa atgattctga gcaattcttc
     6781 tgactgcatc taccaatcta tcaatctctt ccttggtatt ataaaaagaa agggaaggac
     6841 gaacagtaga ctctacacca tatcgtcgaa gagaaggttg tgcgcaatga tgaccagcac
     6901 gcacagcaat tccctctaaa tctaaaagtc ttccaacttc agatgtttgt ttattttgca
     6961 atacaaagga aagaacccct actttattgg gagcggttcc tatcaaacgt aaacctttta
     7021 tttgagtcaa accagctgta gcatactcca aaagagaatg ttcatattta gctatgttct
     7081 ctagacctat gctattcaca taatccaaag cagcacccaa acctactgca tctgctatat
     7141 tcggagttcc tgcctcaaat ttagctggta aatcactata agttgtttcc tcaaaggtta
     7201 cattttttat catattccca ccaccctgcc aaggaggcat taactctaaa atttcttttc
     7261 ttccataaac aacaccgatc ccagtaggac caaagatttt atgtccagag aaaacaaaga
     7321 aatcagcttc caaatcttga acgttaactg gaatatgtgc gaccgactgc gccccatcga
     7381 ttaacacttt tgctccatgc cttttcgcaa gtcctatcat ttcttttaca ggtagaattg
     7441 ttccaagact atttgatgct tgcgtcaaag atacaaattt agtcctcgga ccaaggagtc
     7501 tctcataatc ttctagaatt acttctcctc tctcattcag tggaataaca cgaatgatgg
     7561 cacccttctc ctttgctaca atttgccaag ggacaatatt tgcgtgatgc tctgcttgag
     7621 atagaatgat ctcatcccct gcttgaaaat actttcttcc gtatgtttga gcgactaaat
     7681 tgatcgcctc cgttgttcct ctaacaaata taatttctga tgtggatgaa gccttaataa
     7741 aattctttac tttctctctt gcatcctcga atgcatctgt agatcgagca gctaacgtgt
     7801 gcgctcctct atgtatattt gaattatcat ttgagtagta atgactgatt gcatcaatca
     7861 caattttcgg tttttgcgtt gttgcagcat tgtcaaacca agctagtggc tttccatgca
     7921 ctttctgatt taacactgga aaatctttcc gaatctgatt cacatcaaat ggttgattgg
     7981 aataaatatc ttttacaaat ctagaatcac tctgtttttg aaaaaaaaca caattatcca
     8041 atccattcca atttaaatta tgattcgagt ttttaccttg aatactttcg acaaatgatt
     8101 tcaggttatc atactttctt tcaccaacaa tttctgattg ctgtttcttt aaagtcccaa
     8161 cggattcaga aatcgttttt tgatccaaat tcaacttttc agaaacagac tcatttctat
     8221 ctgtaatttc cgaagtattt tttccaaata ctatctcttg gattttctca aaatctaaat
     8281 ctttttcttt tccagaaaaa gatttttcag gcatatattt attagccgct actttaaaag
     8341 aactccccga aatactattt gctttcggaa gttcattata aagctttgaa gctatttgcg
     8401 caataaaact tgtatcgaat ggttccatag tatctcctag tctactttgg tagagaattt
     8461 ctattctgat aatcgtgata atatccgact tcaacattct ctaaaacagc taacgcatca
     8521 tccgttaaga caacagcgga tgagtagagt gttaataggt aagaagctac tccaagatta
     8581 tctaatccca tgagtcgtgc ggagagactt ggagaaattt caccaggtat tccaatctga
     8641 tgaagaccaa caacaccctg ctcttcctct cccacacgga gtaataatat acttgtagtt
     8701 ccaaaccact ggttcgataa attcgtattt ttaacttcta gtttatcaca tgggataatt
     8761 ggtataccac gccatgtgat aaccggactt ccaaatattt gagttgtaac tggtggaact
     8821 cccctccaag tgcattctct ttcaaacgca gcaatcgctt tgggatgcgc tagaaaaaaa
     8881 gctggttttt tccaaaccaa cgcaagtagt tcatctaaat cgtcaggcgt tggagctcca
     8941 tatcgtgtgc taattctcat agatggatca acagaatgga gtaatccaaa ttttttactg
     9001 tttattagtt cccattcttg tctttctttg attccttcaa tcgttagccg catttgctct
     9061 tctaattgat tgtagggatt attatacaaa tcagaaactc gtgtgtgaac tctaactacg
     9121 gtttgaattg catttaaaga atattctcgg ggtttatctg aataatcaat aaatgtctct
     9181 ggaatttcaa cattttcagc aaaaccagat actaaatcaa tattcctttc accatattta
     9241 tttacggtcg atctcaattc aatgtgttta tcaatcgcct ttttaaaatc ttcttttaaa
     9301 tttggaaact cacttaaaat atcatctaaa tcttttcctg aaagggtaag caatttacaa
     9361 ggagtgatcg ttctaacagt aacgtcagac ggaatatcag acactaattc gatttctccg
     9421 aaatattctc cttcagttag tagtgcaatt ctcaaatcac tgccgtgcac tcctttactt
     9481 agaacctcga cttgcccttg tgctatgata aaaaatttat ttttatcaac cccttctact
     9541 actaatttat ttcctaatgt aacctcttct gtcttaaaac gatttgccat ccgatttata
     9601 atatcatctt ggaattttgc aaacagtggc actgatcgaa gagactgcgg attgaaagat
     9661 actgcgccat ccttgaaatt caattctact ctttctgcct tttttaattc tacttttgtt
     9721 ctgttcactc tatacgttcc accttcaaca tgaacccatg gaagaagact taatagatat
     9781 ctcggggtaa tagacatcat ctgtggacga gtctttgtag tattcgcaag gtttcttgct
     9841 actgacgttg ttacgcttct ttgtaatatg ttatctgaca ttctattctc cttttattta
     9901 aatgcctaat ccatcactaa aatagtttga atttttattt tgatagctat gataatatcc
     9961 cacctccaca ttttctaaaa cagcaatagc atcgtcggtt aagacggctg cggcagaata
    10021 taacgttaat agataggatg ctacccccaa aggatctaaa cccattaatc tagcagatag
    10081 gcttggtaag atttctcccg ggattccagc ttggtgtaat ccaatgactc cttgattttc
    10141 ttccccaact cggactaata gaatatttgt tgttccaagc caaggtctag agtagtaatt
    10201 actttttact tccaatttgt ctgaaggaat aattggaatt cctcgccata gtatcagagg
    10261 tgcaccgaaa gaattctctg tcacaggagg gactcctctc caagtgcatt ccctttcaaa
    10321 tgcagcaatc gctttaggat gagccaaaaa gaatgagggt cttttccaaa ccaaggcaag
    10381 taactcatct aaatcatcag gagttggagc tccataacgt gtgctaattc taaaagctgg
    10441 tgataccgaa tggagtaatc caaatttttt attatttatc aattcccact cttgtctttc
    10501 tttgattcct tcaatcgtta gccgcatttg ctcttctaat tgattatagg ggttattgta
    10561 taaatcagaa actcgtgtgt gaactcgaac tactgtctgg attgcattta aagaatattc
    10621 tctcggagat tctgagtaat caatgaaagt ctctggaatt tcaacatttt cagcaaaacc
    10681 agacacaaaa tcgatatttc tttcgccgta tttattcaca gtggagagca attcaatata
    10741 atcatcaata gcttttctaa aatcatttct caaatttgga aactcattta gaatttgctc
    10801 taagtctgct ccgtataacg tcaataattt acaaggagtt gttgtcttta ctgtgacatc
    10861 tgagggctta tccgaaagca gaccctgttc tccaaaatat tcaccttctg tgagaagtgc
    10921 gattctcaga tcactcccat gaacaccctt gctaaatact tcaacctgac cttgggctat
    10981 aataaagaat ttattcttat cctcaccttc aacaacaaga gtcgttccta aactaacttc
    11041 ctccgtttta aattgattcg aaattcgcgt gagtatatct aatggaagtt tcgaaaaaac
    11101 aggaaccgtt cgaagcgctt ctggattaaa tgataaaaaa acaaatccat ctacaatatt
    11161 taattcaatt cgttctgctc tttttaactc aaccctggtc ctattgactc ggtaagttcc
    11221 accttctact tgcacccaag ggataacact aagtaaccac cttggggtta ttgacatcat
    11281 ctgtggatgt gtctttgttg tattggtcaa atttttagcc actgatgttg tcacactacg
    11341 ctgcaaaata ttatctgaca tattctaaac tccttactat catattctat tattctatat
    11401 acctgatcca aaataaaaat gctcttgtat tactttcgcc tgcgtaattt tagagttagg
    11461 cggaatatct gtagtaatcc aaacatttcc tccaatggtt gaattcttac caatagtgat
    11521 ccgccccaaa atatttgcgt tagaatagat aactacatta tcttcaataa ttggatgcct
    11581 tggctcattt tttattggat ttccagattc atctaatgaa aacttttttg ctccg
//
LOCUS       DAOQOS010000146        11482 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_2916_length_11482_cov_5.919226, whole genome shotgun sequence.
ACCESSION   DAOQOS010000146 DAOQOS010000000
VERSION     DAOQOS010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 11482)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11482)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11482
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2916_length_11482_cov_5.919226"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..705
                     /locus_tag="PLF66_22200"
     CDS             <1..705
                     /locus_tag="PLF66_22200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016750890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-54 factor"
                     /protein_id="HMZ66817.1"
                     /translation="IAKNIKKLEPYPATLFNSKKIDYIVPDVVVTETDGEFTIHINDE
                     WIPKISVNKEYKSVLMKSCSAQDKEFISTRVNSAQWLIRSINQRRQTLYKVMNSIIDF
                     QIDFFRHGINNLKPLTLKDIADKLNMHESTISRITTNKYVQTSWGILELKWFFSSGLK
                     SAEGGKESSKKIHDMIRNLVKEESEDNPLSDQDIVDIMGKRGIEIARRTVAKYRKILK
                     ILPSNRRKRIRDLKAS"
     gene            706..1668
                     /gene="hprK"
                     /locus_tag="PLF66_22205"
     CDS             706..1668
                     /gene="hprK"
                     /locus_tag="PLF66_22205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012388636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HMZ66818.1"
                     /translation="MSIASISVESIVRDHPELELQLISGERGISKKISNSEINRPGLS
                     LTGFFDFFAYDRIQIFGKGEWAYLNSLTDDKVDIIAEKFFSYPINCIIFTHGNMPKQK
                     IVEKADNLNIPLFVSPMSTHKFITLISDILNKSLAPRTMRHGVLIEVFGIGILLSGKS
                     GVGKSETALELIERGHRLVADDMVEIRRYSESYLIGTCSDILSHHMEIRGLGIINIKD
                     IFGVGSVRDSKLIELIINIEEWSENVDYDRTGLDEETDEILGVKIPFLKIPIKPGRNI
                     PIIVETAAMNQRLKKMGTFGAREFNNKLTNYIEQIKIEKSHVKD"
     gene            1643..1909
                     /locus_tag="PLF66_22210"
     CDS             1643..1909
                     /locus_tag="PLF66_22210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012388637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HMZ66819.1"
                     /translation="MKRVTLKINKEGYGMHARPASLFVKTAAQFPCEITVIRDDIEVN
                     GKSIMGLMMLALSADVEFTIVADGDKEEEALEALKNLVVNDFQK"
     gene            1906..2598
                     /locus_tag="PLF66_22215"
     CDS             1906..2598
                     /locus_tag="PLF66_22215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66820.1"
                     /translation="MKLFKDLTSSGEGKYYIRDLYIFLLSIFISIVLAETIIFSDSSS
                     IDLVDKIFVYIYIIFPLFSLALIISYIYRNIRIRQTGKLRSVIRYRLTLAFVFVSILP
                     SIPIVLFSSNVVGKLVESFYRIDISEALKSAIADLKQSEEEDKKNIFNKGKVLQRVVQ
                     RNFSQMDIIYSKASELGMINSDKFYMGIVKGSKIIAETMPLYSQILIQNLKNQMRLSI
                     IIHYIREIRHFF"
     gene            2682..3722
                     /locus_tag="PLF66_22220"
     CDS             2682..3722
                     /locus_tag="PLF66_22220"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HMZ66821.1"
                     /translation="MIYTENSYNTADLWKEKVPFALRFSLGVFFIMMFGISIIVSFLL
                     ARQISRPIVQLASATQRVSRGELDVQLDLNEEGEMGILIESFNQLTRDLKAKNEELIH
                     IQRIAAWKEVAQRMAHEIKNPLTPIQLSAERIRKRLDNPNREKFDEVVRNGTETIIGQ
                     VRVLEHLVKEFSEFARMPTPILINQQLNPIVEESVKLFMDNVNINFVLKLSKNLPEVF
                     IDKRLFLGVVNNLIKNAVEAIQNHRKEISDSNDSQEELLGTIRISTKLEKKLLRKSVV
                     LTVEDSGPGISPELKEKIFQPYYSTKEGHGTGIGLTIVEKTVYDHHAHLSLDESNLGG
                     CSFKIELPLEDR"
     gene            3722..5065
                     /locus_tag="PLF66_22225"
     CDS             3722..5065
                     /locus_tag="PLF66_22225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010569706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HMZ66822.1"
                     /translation="MKIFVVDDEIEIRKSLRNILEDEDYQVEDFSNSKTLLKALAKER
                     PSLLLLDVWLGKEDGLQILDECKKIYPLLPVVMISGHGTIELAVNATKKGAMDFLEKP
                     LSISKILDTINSVLARQNDSEIPEVKLEFDQIIGQSQAIKKVKFAISQAAATNARVFI
                     YGENGTGKELVAKSIFMNSKRKENPYIEINCAAIPEELIESELFGYEKGAFTGAVDRR
                     IGKFEAANNGTIFLDEICDMSLSTQAKVLRILQEQRFEKLGSSESITVDVRVIAATNI
                     PVEDAIKEGKFREDLYYRLNVIPIIIPPLRERKTDIPLLLDFYIQNTIKENDLAQKKL
                     DNEAIGLLTNHFWPGNIRELKNVVERLCIMTIGDTIKGKDVKEALIGFKKAEEIFQQG
                     DLKKAKEEFERQYIIKTLQLNDANVSKTSKILGVERTHLYRKIKALNIQIDNLSD"
     gene            5065..6240
                     /gene="rlmD"
                     /locus_tag="PLF66_22230"
     CDS             5065..6240
                     /gene="rlmD"
                     /locus_tag="PLF66_22230"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HMZ66823.1"
                     /translation="MKKDCIHFGECAGCDILDYEYKSQLKEKKEEISRLFSDFKNTEI
                     LDTFPSPKEFYYRNKVQLPFGRKKGKYEEILTLGLHARDYSKIIDLKECKIQNETLSD
                     IAWIIRDWAREENYSGYNEKKETGFLKYLLLKKSNFSKEILIGLITGSPREFKKISID
                     KLIEKIKTMLEEKYKNENITIAGILQNINLNNTSMVLGEQERLLWGKRYIKEEISGFK
                     YRVGLSTFIQVNPYQTPRLYNEILKNIPEGVNAIDAYCGIGTISLLIAKKAKSVLAIE
                     SNPHSIDSAKIALKENKVNNVKFLLGDTAKILSKLDGQFDLLVVDPPRAGLDRRCISS
                     IFKLDLSKIIYVSCNPDTLLEDAKYLSEKYILKKITPVDMFPQTFHIESVALFERRR"
     gene            6237..6455
                     /locus_tag="PLF66_22235"
     CDS             6237..6455
                     /locus_tag="PLF66_22235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004450172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HMZ66824.1"
                     /translation="MKLTIYVKPNSKKIGIDIINEKEWIVRVKEPATEGKANDAVLYS
                     IAEKLNLPKSKVKLIKGEKSKQKIVEIL"
     gene            6502..7152
                     /locus_tag="PLF66_22240"
     CDS             6502..7152
                     /locus_tag="PLF66_22240"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HMZ66825.1"
                     /translation="MMMEFIYRIVIIFFLLNLAINAQNQAPEETKQFRFVIAKTGLTL
                     RANPDTESEAIDTIPFGKIVEVVNELNETVNVQGNVSNWFEVKYKWNKGYVVSGFLSV
                     SSENPDKKGIKQKDYYATWKGDWKCGKEYSNLKIKKNGSFSGWLFAGGDDSGCGGTSI
                     KGNWSINSLGEICLKTSSDESCFFIYNNRLIANLNSASGFKENYEAEILSGLSKVK"
     gene            7149..7976
                     /locus_tag="PLF66_22245"
     CDS             7149..7976
                     /locus_tag="PLF66_22245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66826.1"
                     /translation="MNTDNIYSQFASILSDVKFLMDKNEFEIFRLPDEKNPYSYQFKW
                     NQIFNDSDKQKQALIEKPIDAAENIRCKLCPKKIAGIRNYIHKGNKPVLVLHYTGEFR
                     KGQPSLSKTNPSLALRTRDSEDLFDRLIKKVFNFSSKEFFFQEYPACSFNHNTSTLAD
                     WKTRMEACDLHVKNTILANNIKGIILMGSAAVMRLGAEQAKEKTGKLEPLSFQEVEIP
                     TLVIRSPEGVFSLEEKRKKLEHNKTSEGYKLAKKEEDEVKIGLVEHLTLFKNKIGII"
     gene            7973..9934
                     /gene="priA"
                     /locus_tag="PLF66_22250"
     CDS             7973..9934
                     /gene="priA"
                     /locus_tag="PLF66_22250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000641110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HMZ66827.1"
                     /translation="MIEYAEVAINLPIKDEDTLTYEIPFGTKNIEVGKRVEIEVRNRK
                     MEGVIIDIHNVMPSYKTKPIGRVIDKQPVITKDQIELGKWMKDFYVSTLGESLYKMIP
                     SGRRNISNDKIDITVESKLLNLNPEQENAYRKIRSDFGKESIHLLYGITGSGKTEVYI
                     HLMHTLLNETNKSAILLVPEISLTVQILKRLQLIFGSSLAMLHSAMKVSDKFRNYQQI
                     LRGEKRIIVGTRSAIFAPVKDLGLIILDEEHDGSYKEHSNPRYHARQIAMQRAKMGNA
                     VVVLGSATPSVESFYHAKRGNIQFHTLKNRAKSAELSKVFLQEKKEDKEIISDTLLFK
                     IKQRLDKKEQVVLLLNRRGHSPLIYSKSEKKILECPNCSTNLCYHNKGKAVCHLCGYN
                     ESYSKLNDRYQNQLDLMGAGTQKLEEYLLEKFPNVLIERLDQDATKNKEILADVIGRL
                     IERKIDILTGTQMIAKGLDASHVTLVGVINANTGLGLPDFRASERVYSLLTQVAGRAG
                     RSELKGEVIIETFNSSHPVIQFATRQNYDDFFDHEILVRKDLHYPPFCRLIRLLTRSK
                     IETKSADAIELIRNELDKKISNLNLKDVYILGPAPAPFYKIDSNFRNHIVIKTNSLQR
                     VRDVIRELKMISLPSNVYLEIDIDPMDLV"
     gene            10122..10871
                     /locus_tag="PLF66_22255"
     CDS             10122..10871
                     /locus_tag="PLF66_22255"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HMZ66828.1"
                     /translation="MDSNYHDLYRLKETTEITVYLAYSEKDQKKVILKILNPEYPTQE
                     QVSIFHNEFDLTLDLNSKYIRKPIRKERINGKRVLVFEYINGKSLYDIYQDAKKIDLE
                     KFITISIEIAKAISEIESLGIIHKDLNSNNILYAEDTNQITIIDFGIATKISSKSYYL
                     GNPEKLEGTLSYISPEQTGRMNRAIDFRSDLYSLGISLYEILAGKLPFVFTDSLNIIH
                     AHMAVKPVDLFEHINMSNLRFTKKYSNKAIE"
     gene            10887..>11482
                     /locus_tag="PLF66_22260"
     CDS             10887..>11482
                     /locus_tag="PLF66_22260"
                     /inference="COORDINATES: protein motif:HMM:NF024589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HMZ66829.1"
                     /translation="MHLIEKNAEERYQSASGFLFDLIQVKEILEGRRKETDFILASKD
                     FSAQFRIPEKLFGRTKEIADLHEAYRTILDGKKPFILISGYSGVGKSALVHEIHKPSI
                     ELRGLFIEGKFDQYQKNLPYSAWIQSFKEFVKNILTENEISLEKWKNIILDAVGTNGK
                     VLTDIVPDLQFIIGHQPDVIELGPVETVNRFNTVLTNF"
BASE COUNT         4336 a         1532 c         2104 g         3510 t
ORIGIN      
        1 attgctaaga atattaaaaa gttggaacct tatccggcaa cattatttaa ttctaagaaa
       61 atagattata ttgttcctga cgttgttgta acggaaacag atggagaatt tacaattcat
      121 atcaatgatg aatggatccc aaaaatttct gtaaataaag aatataaaag tgttctaatg
      181 aaatcttgta gtgctcagga taaagaattt atatctacgc gcgtaaattc tgcacaatgg
      241 ttgatacgtt caatcaatca gagacgtcaa acgctttata aagtaatgaa ttcaattatt
      301 gattttcaaa ttgatttctt tcgacatggg attaataatt taaaaccgtt aactttaaaa
      361 gacattgcag acaaattaaa tatgcatgaa tctaccatat ctagaataac taccaataaa
      421 tatgttcaaa catcttgggg tattttagaa ttaaaatggt ttttctcttc aggattaaaa
      481 tcagcagaag gtggtaaaga atcctctaaa aagattcatg atatgatacg aaatttagtg
      541 aaagaagaaa gcgaggataa tcccctatct gatcaagaca ttgtagatat aatgggtaag
      601 cggggaattg agattgcgag aagaacggtt gcaaaatata gaaaaatttt aaagattctt
      661 ccatcaaaca gaagaaaaag aatacgggat ttaaaggcaa gttagatgtc aatagcaagc
      721 ataagtgttg aaagcattgt ccgcgatcat cccgagttag agttacaact cattagcggt
      781 gaacgcggca ttagtaaaaa gatttcgaat tcagagataa atagacctgg tctttcgctt
      841 accggttttt ttgatttctt tgcttatgat agaattcaaa tttttggtaa aggagaatgg
      901 gcatatttaa attctctgac tgatgataaa gtggatatta ttgcagagaa atttttctct
      961 tatccaatta attgtatcat ttttacacat gggaatatgc ctaagcagaa gatagtggaa
     1021 aaagcggata atctaaatat acctctattt gtttccccta tgtctactca taaatttata
     1081 actctgattt ctgatatttt aaataaaagc cttgctccta gaacaatgag gcatggtgtt
     1141 ttaattgaag tattcggtat tggaatttta ctatctggaa agagtggggt aggaaaaagc
     1201 gaaactgcac tggaactaat tgaaagaggg catagacttg ttgcagacga tatggtagaa
     1261 attagacgtt attcggaaag ctatcttatt ggaacttgct ccgacatttt aagtcatcat
     1321 atggaaatca gaggtcttgg gattataaat ataaaagata tttttggggt aggatctgtt
     1381 cgggatagta aattaataga attgattata aacattgaag aatggtcaga gaatgttgac
     1441 tatgatcgaa caggtttaga tgaagaaaca gatgagattc ttggagtcaa aatcccattt
     1501 ttaaagattc caattaagcc cggaagaaat atacctatta tcgtagagac tgcggcaatg
     1561 aaccaaaggc taaagaaaat gggaacattc ggagcaagag aatttaataa taaattaaca
     1621 aattatatag agcagataaa aattgaaaag agtcacgtta aagattaaca aagaaggcta
     1681 tggaatgcac gcaagacctg catctctatt tgtaaagaca gcagctcagt ttccctgtga
     1741 aataaccgtt attagagatg atattgaggt aaatggtaaa agtattatgg gtctaatgat
     1801 gttagctctt tcggctgatg tggaatttac aattgtcgca gatggtgata aggaagagga
     1861 agcactagaa gcacttaaaa atctagtcgt aaatgatttt caaaaatgaa attgtttaaa
     1921 gatttaacat cttccggaga aggaaaatat tatataagag atctgtatat ttttctttta
     1981 agtatattta tatctattgt attagcagaa actattatat tttctgattc ctcatcaata
     2041 gacttagttg ataaaatatt tgtttatatc tatattattt tcccgctctt ttcccttgct
     2101 ttaatcatat cctatatata tagaaatatc agaattcgtc aaacaggtaa attaagaagc
     2161 gttatacgat atagattgac acttgctttt gtttttgttt ctatactacc ttctattcca
     2221 atcgttctgt tttcttctaa cgtggttggt aaattagtag agagttttta tcgtattgat
     2281 atttcagaag ctttaaaatc agctattgcc gacttaaaac agtcagaaga agaagacaaa
     2341 aaaaatattt ttaataaagg caaggttctg caaagagtag ttcaaagaaa tttttctcaa
     2401 atggatataa tttattcaaa agcgtccgaa ttagggatga ttaattcgga taaattttat
     2461 atgggaattg taaagggaag taaaattatt gctgagacaa tgcctcttta ttcacagata
     2521 ttaatccaaa atttaaaaaa ccagatgagg ctttcgataa ttatacatta tatcagagag
     2581 ataaggcatt ttttctagca aaaataaatt cgaaagaacc agataatttt atcattatag
     2641 gtaagagggt tcatataggg aatgaaaaga atgcctataa tgtgatctat acagaaaatt
     2701 cttataatac agctgatttg tggaaagaga aagttccttt tgctttaaga ttttctcttg
     2761 gtgtattttt tattatgatg tttggtattt cgattatcgt ttcattttta ttagcaagac
     2821 aaatttccag accaatcgtt cagttggcga gtgcaaccca aagagtatct agaggggaat
     2881 tggatgtcca attggattta aatgaagagg gggaaatggg aattttgatt gaatctttta
     2941 atcaattaac tagagatttg aaagcaaaaa atgaagagct aattcatata caaagaattg
     3001 cagcttggaa agaagtggca cagagaatgg ctcacgaaat taaaaatccg ttaactccaa
     3061 ttcaactctc cgccgaaaga attcgaaagc gtttagataa tccaaaccgt gaaaaatttg
     3121 atgaagtggt aagaaatgga acggaaacaa ttattgggca ggttcgagtt ttagaacatc
     3181 ttgtaaaaga attttctgag tttgctagaa tgccaacacc gatccttata aatcaacaac
     3241 tgaatccaat tgttgaggaa tcggtaaaac tgtttatgga taatgtaaat attaattttg
     3301 ttttaaaact ttcgaagaat ttaccggaag tgtttattga taaaagatta ttcttaggag
     3361 ttgtaaataa tctgattaaa aatgcagttg aggcaattca aaatcatagg aaggaaatct
     3421 ctgattcaaa tgattcacag gaagagttac tcggaacgat tcgaattagc actaaattag
     3481 aaaagaaatt acttagaaaa agtgtcgttc tgactgttga agatagtggt cctggaattt
     3541 cacctgagtt aaaggaaaag atttttcagc cttattattc cactaaagaa ggtcatggaa
     3601 ctggaatagg attaacgata gtcgaaaaaa ctgtttatga tcatcatgcg catttgagtc
     3661 tagatgaatc aaatttaggc ggatgtagtt ttaaaataga gcttccactt gaggatagat
     3721 aatgaaaata tttgttgttg atgatgaaat agaaattaga aaatctctgc gaaatatttt
     3781 agaggatgaa gactatcagg tcgaagattt ttcaaatagt aaaacattgc taaaagcact
     3841 cgcgaaggaa cgtccctcat tattgctttt agatgtttgg cttgggaagg aagatggttt
     3901 acagatttta gatgagtgca aaaaaatcta tcccttgctg cctgtagtga tgatctcagg
     3961 acatgggaca atagagttgg cggttaacgc taccaaaaaa ggcgcaatgg actttctaga
     4021 aaagccactt tctatttcta aaattctaga cactattaat tcagtcttgg cgcgccaaaa
     4081 tgattccgaa ataccagaag taaaattaga atttgatcag attattggtc agtctcaagc
     4141 aataaaaaaa gttaagtttg caatttctca agcggctgct accaatgctc gagtttttat
     4201 ctatggagaa aatggaactg gaaaggaatt ggttgccaaa tctatattta tgaattcaaa
     4261 gagaaaagag aatccatata tagaaataaa ctgtgctgca attccagaag aattgatcga
     4321 gtctgaattg ttcggatatg aaaaaggtgc atttactgga gccgttgatc gccgtatagg
     4381 aaaatttgaa gcggcaaata atggaacgat tttcttggat gaaatatgcg atatgtcttt
     4441 atctacacaa gcaaaagttc ttagaatact acaagaacaa agatttgaaa agcttggtag
     4501 tagtgaatcc atcactgtag atgtaagggt tattgctgct acaaatattc cagtggaaga
     4561 tgcaattaaa gagggaaagt ttagagaaga tttatattat cgactcaatg taatacctat
     4621 tattattcca cctcttagag aaagaaaaac agatatacca cttttactcg atttttatat
     4681 acaaaatacc atcaaagaaa atgatttagc acaaaagaaa ttagacaatg aagctatcgg
     4741 tcttttaaca aatcattttt ggccaggtaa tatacgtgag ttaaaaaacg ttgttgaaag
     4801 attgtgcatt atgacgatag gcgatacaat taaaggaaag gatgtgaaag aggctttaat
     4861 cggattcaaa aaagccgaag agatttttca acaaggagat ctaaagaaag caaaggaaga
     4921 atttgagaga cagtatatta taaagacctt acaattaaac gatgcaaacg ttagtaaaac
     4981 ttcaaagata ctaggtgtag agagaaccca tctttataga aaaattaaag ctcttaatat
     5041 tcaaatcgat aatttgagcg actgatgaaa aaagattgta ttcatttcgg agaatgtgcc
     5101 ggttgtgata ttttagatta tgaatataaa tcacaactaa aagaaaagaa ggaggagatt
     5161 tctagacttt tctcggattt taaaaatacg gaaatactag atacatttcc aagtcctaaa
     5221 gagttctatt acagaaataa agtccaactt ccatttggac gcaaaaaagg aaagtatgag
     5281 gaaattctta ctcttggttt acatgcgagg gattattcaa aaataattga tctaaaagag
     5341 tgtaaaatac aaaatgaaac gttatctgat attgcttgga tcattagaga ttgggcaaga
     5401 gaggaaaact attcgggtta caatgaaaag aaggaaaccg ggtttttaaa atatttactt
     5461 cttaagaaaa gcaatttttc aaaagaaata ttaatcggac ttattacagg atcacctaga
     5521 gagtttaaaa agattagcat tgataagcta atcgaaaaaa taaaaactat gttagaagaa
     5581 aaatataaaa atgaaaatat aactatagcc ggaattttac aaaacattaa tttaaacaac
     5641 acatctatgg ttcttggaga acaagaaaga cttttgtggg gaaagagata tatcaaagaa
     5701 gaaatttccg gttttaaata tagagttggt ctttctactt ttattcaagt aaatccctat
     5761 cagactccta gattgtataa tgagatttta aaaaacattc cagaaggagt taacgctatt
     5821 gatgcctatt gtggaattgg aactatctct ctcctgattg ccaaaaaagc aaagtcagtg
     5881 cttgcgattg aaagtaatcc acactccatt gattcagcaa aaattgctct taaagaaaat
     5941 aaagtaaata acgtaaaatt cttgttaggc gatactgcaa agattctttc taaattagat
     6001 gggcaatttg atttattagt tgtggatcca cctagagctg gattggacag aagatgcata
     6061 agttcaattt ttaaactaga tttatctaaa ataatttatg tttcttgtaa tccggataca
     6121 ctcttggaag atgcaaaata tttatcagag aaatatattc taaaaaagat tacaccagta
     6181 gatatgtttc cgcaaacatt tcatatagaa tcggtcgctt tattcgaaag aagaagatga
     6241 agttaactat ttatgtaaaa ccaaatagca agaaaattgg aattgatatc atcaatgaaa
     6301 aagaatggat tgttcgcgtc aaagagccag caacggaagg aaaagcaaat gatgcagttt
     6361 tgtattccat cgcagaaaaa ttaaatctac cgaaatcaaa agtaaaatta attaagggag
     6421 aaaaatctaa acaaaaaatt gtagaaattc tttgaacgaa aaagaatata cagagagatt
     6481 agttttggga tttagtattg tatgatgatg gaatttattt atagaattgt aattatattt
     6541 tttctcttaa atctagctat caatgctcaa aatcaggctc cagaagaaac gaaacaattt
     6601 cgctttgtaa ttgcaaagac tggactcaca ctaagagcaa atccggacac tgaatccgaa
     6661 gcgatagata ctataccttt tggcaaaatt gtagaagtgg taaatgagtt aaatgaaact
     6721 gttaatgttc aaggtaatgt ttccaattgg tttgaagtta aatataaatg gaataaaggt
     6781 tatgttgtaa gcggtttttt aagtgtatcc agtgaaaatc cagataaaaa gggaatcaaa
     6841 caaaaagatt attatgcaac ttggaaagga gattggaaat gcggaaagga gtattccaat
     6901 ctaaaaatta aaaagaatgg gagtttttct ggttggctct ttgcaggtgg agatgattcg
     6961 ggttgtggag gcacatcaat aaaagggaat tggtctatca attcattggg agaaatatgc
     7021 ttgaaaacaa gttcggatga atcttgtttt tttatttata ataatagatt aattgcaaat
     7081 ctgaattcag cctctggttt caaagaaaat tatgaagcag aaatattaag tggtctttca
     7141 aaagtaaaat gaatactgat aatatttaca gtcaatttgc atcaattctt tctgatgtaa
     7201 aattccttat ggataaaaat gagtttgaga tatttcgcct accggacgaa aaaaatcctt
     7261 attcatatca atttaaatgg aaccaaatat ttaatgattc agacaagcag aagcaagcgt
     7321 taatagagaa accaattgat gcagcggaaa atataagatg taagctgtgt cctaaaaaaa
     7381 ttgcaggtat tcgaaattat attcataaag gaaataaacc tgttttagta ttacattata
     7441 caggagagtt cagaaaagga caaccctctc tttctaaaac aaatccatct ttggctctta
     7501 gaactagaga ttctgaagac ttatttgatc gtcttataaa aaaagttttt aatttctcct
     7561 ctaaagaatt cttttttcaa gagtatcctg cttgtagttt taaccataac acatcgactt
     7621 tagctgattg gaagacaaga atggaagctt gcgatttaca tgttaaaaat actattttag
     7681 caaataatat aaaaggtatc atattaatgg gttctgcagc cgttatgcga ttaggtgcag
     7741 agcaagcaaa agaaaaaaca ggtaaattag aacctttatc atttcaagaa gtggaaattc
     7801 ctactttggt gattagatct ccggagggag tattttcttt agaagaaaag aggaaaaaat
     7861 tagaacataa taaaacgagc gaaggatata aattagccaa aaaagaagaa gatgaagtga
     7921 aaataggtct agttgaacac ctaacgttat ttaaaaataa aataggcata atttgataga
     7981 atacgcagaa gtagcaatca atcttccaat taaagacgaa gatacactta cctatgaaat
     8041 tcccttcgga acaaaaaata tcgaagtagg aaaacgtgta gaaatagagg taagaaaccg
     8101 aaaaatggaa ggagtaatta ttgatattca caatgttatg ccttcctata aaactaaacc
     8161 tattggaaga gtaattgata agcaacccgt aattacgaaa gatcaaatag aacttggaaa
     8221 atggatgaaa gatttctacg tttctacgtt aggtgaatcc ttgtataaaa tgattccgag
     8281 cggtagaaga aatatttcta acgataaaat tgatataact gttgaatcta agttattaaa
     8341 tttaaatcca gaacaagaaa atgcgtatcg aaaaattcga agtgattttg gtaaagaatc
     8401 gattcattta ctttacggaa ttacagggag cggcaaaaca gaagtatata tacatttaat
     8461 gcatacactt ctaaatgaaa caaataaatc tgctatacta cttgttcctg agattagttt
     8521 aacagttcaa attttaaaaa gactccaatt gatatttggt agctctctag caatgttaca
     8581 ttccgcgatg aaggtttctg acaaatttag aaattatcag caaatattaa gaggtgagaa
     8641 gcggataatc gtcggaaccc gcagtgctat ttttgctcct gtaaaagatc taggtcttat
     8701 tattttagat gaggagcatg acggttctta taaagagcat tctaatccta gatatcatgc
     8761 aagacaaatt gcaatgcaaa gagctaaaat gggaaatgca gttgttgtac ttggatctgc
     8821 tactccttct gttgaatctt tctatcatgc aaagaggggt aatattcaat ttcatacctt
     8881 aaaaaataga gcgaaatcgg cagagttatc aaaagtattt ttacaggaaa aaaaagaaga
     8941 caaagaaatt ataagcgaca cgctcttatt taaaattaag caaaggcttg ataaaaagga
     9001 gcaagtagtt ttacttttaa atcgaagagg gcacagtcct cttatctatt ctaaatcgga
     9061 aaagaaaatt ctggaatgtc ctaattgttc taccaatctt tgttatcaca acaaaggaaa
     9121 agctgtttgc catttatgtg gttataatga gagttattcg aagctaaatg ataggtatca
     9181 aaatcaattg gatttgatgg gtgctggaac acagaaatta gaggaatatc tactagaaaa
     9241 atttccaaat gttttaatag aaagactcga tcaagacgcc actaaaaata aagaaattct
     9301 tgcagatgta attggtaggt tgatagagcg taaaatcgat attttaacag gaactcagat
     9361 gatcgctaag gggcttgacg catcacatgt aacccttgtt ggtgtgatta atgcaaatac
     9421 tggtttggga cttcctgatt ttagagcgag tgagcgcgtt tattctctac ttactcaagt
     9481 ggcaggtagg gcaggtagat ctgaattaaa aggggaagta ataatcgaaa cttttaatag
     9541 cagtcatccg gtgatacaat ttgcaacaag acaaaattac gatgatttct ttgaccatga
     9601 aattttagtt agaaaagatt tgcattatcc acccttttgt agattgataa gacttcttac
     9661 aagatctaaa attgaaacaa aatcagcaga tgcaatagag cttataagaa atgaattaga
     9721 taaaaaaatt tctaatctca atttgaaaga tgtatatatt ttaggacctg cacccgcacc
     9781 cttttataaa attgattcta attttagaaa tcatattgtt ataaagacca attcactgca
     9841 gagagtaaga gatgttatac gcgaactgaa aatgatttct ctcccatcta atgtatatct
     9901 agaaatcgat atagatccca tggatttggt ctaataaatc tctcggaaaa catacaagta
     9961 ttttctcgaa taaaaacgga gatatactgt agaagacatt ataataaaat aaaacaatgg
    10021 cgttgcataa cgctaatatt taaaataata tttcttataa agtaatttgc aataaacgat
    10081 aattcagact tgatgtaagt gaatttattt tttttatatt attggattca aattatcacg
    10141 atttatatag gctgaaggaa actacagaaa taactgttta tcttgcctat tcagaaaaag
    10201 accaaaagaa agtaatttta aaaattctaa atccagaata tcctacgcag gagcaagttt
    10261 ccatttttca taacgagttt gatttaacgt tagatttaaa ctctaaatat atacgtaaac
    10321 caattcgaaa agaaagaata aatggaaaaa gagtattagt atttgaatat ataaatggta
    10381 aatctctcta tgatatttat caagatgcaa agaaaattga tttagaaaaa tttattacaa
    10441 tcagtataga aattgcgaaa gcgatatctg aaatagaatc acttggtatt attcacaagg
    10501 atctcaactc aaataatatt ttatatgcag aagatacaaa tcaaattaca attatcgatt
    10561 ttggaatcgc aaccaagata agctctaaaa gttattatct aggaaatcca gaaaaattag
    10621 aagggactct ttcttatatc tctcctgaac agacaggtag gatgaatcga gctatagatt
    10681 ttagatcaga tctatactca cttggtatta gtctttatga gatactagca ggaaaattac
    10741 cgtttgtatt tacagattct ctaaatatta tacatgcaca tatggctgtt aagcctgtag
    10801 atttatttga acatataaat atgagtaatc taaggtttac taagaaatat tcaaataaag
    10861 caattgaatg attatccaat ataattatgc acctaataga gaagaatgca gaggaacgtt
    10921 atcagtccgc ctctggattc ctttttgatt tgatacaagt taaagaaatt ttagaaggca
    10981 gaagaaaaga aacagacttt atattagcaa gtaaagattt ttcagctcaa tttagaattc
    11041 cagaaaaact ttttggcaga accaaggaaa ttgcagatct acatgaagcc tatcgaacca
    11101 tactagatgg gaaaaagcca tttattttaa ttagtggata ttctggtgtc ggcaaatcag
    11161 cgttagttca tgaaatacat aaaccaagta ttgaattaag aggacttttt attgagggaa
    11221 aatttgatca atatcaaaaa aacctaccat attctgcctg gattcagtca tttaaagaat
    11281 ttgttaagaa tattttgaca gaaaatgaaa tctctctaga aaaatggaaa aatataatcc
    11341 tagatgctgt gggaactaac ggtaaagttt tgacagatat agttcctgat ctacaattta
    11401 ttatcggaca tcaaccagat gtaatagaat taggtccagt ggagacagtg aatcgattta
    11461 atactgtcct tacaaatttt at
//
LOCUS       DAOQOS010000147        11363 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_2948_length_11363_cov_6.957817, whole genome shotgun sequence.
ACCESSION   DAOQOS010000147 DAOQOS010000000
VERSION     DAOQOS010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 11363)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11363)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11363
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2948_length_11363_cov_6.957817"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1618
                     /locus_tag="PLF66_22265"
     CDS             <1..1618
                     /locus_tag="PLF66_22265"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HMZ66830.1"
                     /translation="ATQVYRLRVEKRRYEIILRQERDRINEIAYITALGDLKNHLFRL
                     NQTEKICETLNEFIQEYFQVEHSSVYLWSDEEGAFYPHPVRKDSKRFFVYDPFMLWLT
                     DNDQVLSRDHFEKAPAYSTIKEDAIRILDSVHSDIVIPLTLNASLLGILFLGKRQDGS
                     TMTSEDLDRLYEIKSTSVMSLSNAIFYARSTALTENLENKVKERTKALEEAQAQLVMS
                     EKMASLGVMVAGIAHEINTPAGVINGAADNMEANLHYLIRQYPEVIQYFNDEVFTQKY
                     INVIEQILIDNSRETLDPKDRFKKKREIRERILGQGLSSMLADDFATFLVEKNFLQQE
                     ELILDLVKVGGAKILELIKHTTGINRNLKNIKYAIKNIVRIVRALKYYSHLDQAAYAE
                     ADLIEGIENTLIIVHNQLKMGIQIERNFQPIPLVPCNLDELNQVWTNIIQNAIHAMKG
                     KGILKISSYLEEPFVTVEIQDNGTGIKPEILDRIWDPFFTTKDQGEGSGLGLGIVKGI
                     IEKHKGKISATSKPGETIFKIQLPYTNSQK"
     gene            1783..3060
                     /locus_tag="PLF66_22270"
     CDS             1783..3060
                     /locus_tag="PLF66_22270"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HMZ66831.1"
                     /translation="MEPFIPASFNLALNWTADLLFPVLLKAVQNINEVIITDEDKEML
                     RSLKKERLLFFTNHPTTAEPPIAYHIANLMGTRFKFMASRQVFEWNGGMVGKVISGIG
                     AFSVIAGIPDRDSLKAARSALAAKEGKLVLFPEGEPTSGENDNLMPFQPGLAQLGFWG
                     YEDAKKIDPNADILVLPGFMKYIVDATEKEIRNDLEESVQKIEKHYKIDPGRKNILRR
                     FLTIGRYMVEEAESEYNIKDGDKHDFDYRVGRIRHAILDNCADKFQIKGYKRDDDAIH
                     KLRHLFAVMEMVMIKYPDETLPKLSSKEIEWFKRECVKAFDFIVIKGDYLASNPTPER
                     FYEWLARYESYIFGKIPRALGGEASHLPRKAYITFKKPFRLSEYYAEYKTKKRGAIDA
                     MMARLRADIQELLDSSMYLSRPLVKPFDTESLV"
     gene            complement(3325..4746)
                     /locus_tag="PLF66_22275"
     CDS             complement(3325..4746)
                     /locus_tag="PLF66_22275"
                     /inference="COORDINATES: protein motif:HMM:NF019268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HMZ66832.1"
                     /translation="MKNIFYLIKFKFFLILPSLLFVSSILAQESSNTDSKTVNNKQEE
                     TKQSKTIDSSQPEQTIDTRVLNPKDKEQEIKIDDKKLNTKLPIDFGVFVDGYYNANLN
                     RPTATAQNYTTQAIQNNSFAINLAHIEGKLDAEKFRARLALQTGTSVNANYSNETTNQ
                     KYSNQNSVRNIQEAYVGIKLGKRTWIDAGIYMGNIGFEAWVSSYNWVYTRAIVLDNVP
                     YYSTGVRISHEVTDKLSVQLHIMNGWQNITDNNKDKALGMQVAYKITDKMKFTYNNFI
                     GNETSAPTKTDAIGTVYMYSKGLGYTRNTRYYQNFIYHYDITDRFSIAASFDIGFQIR
                     GTAEKYNPAIPSSINPNYPYYTAHSNAYKRWYDGTLWVTYRLFPEWRISGRLERYVDR
                     EGANVPVVKQSAIFSKEPGKKPNGFEVGAISLNLDYIPNEYAMIRFEVKYRKSSDPLF
                     DYADSADLSRHEKMFISAMTFKY"
     gene            complement(5158..5682)
                     /locus_tag="PLF66_22280"
     CDS             complement(5158..5682)
                     /locus_tag="PLF66_22280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66833.1"
                     /translation="MRNYFYIIILLYTYCSSTGSKIETEKNDDFYEMTFAPCVCQKSE
                     LIHQARQAIILESIKNYKANNKTELEPEIEKNIIEQNESFLYDFSLDTKPKETELEPI
                     VTATGKVNQEKLDQYVKEQMIHKPNRNTNTSEYRSRGLEVIKGFKSDRRKRQTVDEIN
                     KNKSIHIYEIRKKD"
     gene            5719..6888
                     /locus_tag="PLF66_22285"
     CDS             5719..6888
                     /locus_tag="PLF66_22285"
                     /inference="COORDINATES: protein motif:HMM:NF025224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HMZ66834.1"
                     /translation="MKYALHTFKYLEFIVMRFLFFFIFIIFNLFPESPSVEDSELKKG
                     KVFSSFDDALKNPEKIYRLNLSNTRMNEIPSDIEKFTSLQELSLSSNKIEFLPDQICK
                     LKKLQKLNLSYNHLKMLPSCFGELDQLQELNLTANEMLSLGPEIGLLKKLEHLTLLEN
                     TLQELPKEFGKLANLQNLDIRKNKLRELPEQISELKSLKTLNLASNQITKLPSGIGGL
                     SALRFLDLTSNKITILPESIGLLNNLEELNLSSNNLGLLPSSFGNLSELKKINIEKNS
                     LISLPSEISNLKKLQRIYLSHNSLDKFPEGLTNLGEVLSIEINNNRITHLPDSIGSMT
                     MLQILNLSSNKITSVPESFGDLKRLTTLYLKKNPFSKPEQEKLKKLMPNCKIYWD"
     gene            complement(6885..8195)
                     /locus_tag="PLF66_22290"
     CDS             complement(6885..8195)
                     /locus_tag="PLF66_22290"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HMZ66835.1"
                     /translation="MNTKKENISFSVYASTPEVIDEIITAAQMAEDNVRSFHPKVFYQ
                     IDKAIDYLTYGQITSRLFVIEHTGQFDFLAKTVNQVHNDPWLHGTVIVIIADKLTEAE
                     IVKLLDIGVIDFMTSNEVRLKIPTILKIVTSNLELFESEQFLLENITRKKGKIKIKNN
                     LRLVPRVANLIMSICYAAGFRNYEAFSKISLSLHEMIQNAIEHGNCGVGFEMKSKILT
                     DSMDMYGAIDERASYPENINKRVTISYEINNDQAIFTIKDEGNGFDPSAIPSPRSEEN
                     IFAVHGRGILMTRNFVDEMEYNDKGNIVKLIFYNNTEIKRRASHLMQFATGEILFLNP
                     DDILFESGSESDYFYYILSGKLGVYIKNNQRIAVLTPEDMFVGEMSFLHHNKRTGTVK
                     AMTKAQLLPISRSGFIDMVKKYPYYGVFLARLLTKRLILRNRSI"
     gene            complement(8192..9148)
                     /locus_tag="PLF66_22295"
     CDS             complement(8192..9148)
                     /locus_tag="PLF66_22295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HMZ66836.1"
                     /translation="MKRNIRNTLAIIIAFLLTLYISGIFLFSYMILHPHWHHPGLTHL
                     CSQSQLEFSPELCIDHPEDAVKLKFESIQWQTDQFIIDGWLFPASTKSNRVVIFIHGA
                     GADRRNGFKLVPFLLKSDYNVILYDAPNHGRTTNNGLGVSYGIRESIGFIAVLNWAKE
                     KYKHVVVISTSAGTSTFILSKENWIGKVDAAVIENPFYSMERIIKENKISKFLPDFYL
                     EIIFKYVSFKGEFDFKSIKPGELVRDFPEIPIVVFHGTADKTTPYQHGVDLYSNLSIR
                     KKVFFEAKNTGHCRIWDDYPKEFEEKTLKAFEDGINIAKNKL"
     gene            complement(9258..10274)
                     /locus_tag="PLF66_22300"
     CDS             complement(9258..10274)
                     /locus_tag="PLF66_22300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010571233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium chain dehydrogenase/reductase family
                     protein"
                     /protein_id="HMZ66837.1"
                     /translation="MNRLVYRTPKAGSLEDLKLSSESLSDPLEEEVTIEVKAIGLNFA
                     DVFALVGLYSATPKGSFIPGLEYSGNVIKTGAKVKNFKIGDRIMGVTRFGGYVSHLNS
                     NTSYIHPLPSNWSYQEGAAFIAQALTAYYALFPLGNLQKGQTVLIHSAAGGVGIYANR
                     IAKKFDAYTIGTIGDSSKIQTLKSEGYDDYIIRDSNFPTQLKNKLKDRSLNLVLECIG
                     GKIFQSSFDSLSPTGRIVVYGAAHFAPESSSPNFFKLAYQYLTRPRIDPLSMISSNKS
                     VLAFNLIWLWDKMDELSKMLEQILDLKLPPPLIGKTFPFTEALDALYYFKSGKSVGKI
                     ILQT"
     gene            complement(10805..11363)
                     /locus_tag="PLF66_22305"
                     /pseudo
     CDS             complement(10805..11363)
                     /locus_tag="PLF66_22305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808803.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="MMPL family transporter"
BASE COUNT         3656 a         1858 c         1933 g         3916 t
ORIGIN      
        1 tgcaacacag gtttatcggt tgcgtgttga gaagcgccga tatgaaatta ttctcaggca
       61 agaaagagat agaatcaatg aaattgcgta tataactgct cttggtgatt tgaaaaacca
      121 tttatttaga ttaaaccaaa ctgaaaaaat ttgtgaaaca ttaaatgaat ttattcaaga
      181 atactttcaa gtagaacatt caagcgtcta tctatggtca gatgaagaag gtgcttttta
      241 tccacatcca gtaagaaagg attcaaagag attttttgta tatgatccat ttatgttgtg
      301 gctaacggat aacgatcagg ttttatccag agatcatttc gaaaaagctc cagcatattc
      361 tactataaag gaagatgcga ttcgaatttt agattctgta cattcggaca ttgtgattcc
      421 acttacttta aacgctagtc tcttgggaat tttattctta ggaaaaaggc aagatggttc
      481 cactatgacg agtgaagatt tggatcgatt atatgaaata aaatcaacgt cagtcatgtc
      541 cttatcaaat gctatttttt atgctagatc tacagccctt actgagaatc tagaaaacaa
      601 ggtcaaagaa cgaacaaagg ctttagaaga ggcacaagca caacttgtca tgtctgaaaa
      661 aatggcttcg cttggggtaa tggttgcggg tattgcacat gaaataaata ctcctgcagg
      721 ggtgatcaat ggagcagctg ataatatgga ggctaattta cattatctca tacgtcagta
      781 tccagaggtg attcaatatt ttaatgatga ggtctttact caaaaataca tcaatgtgat
      841 agagcaaatt ctgattgaca attctagaga gacattggat cccaaagacc gatttaagaa
      901 aaaaagagaa attagagaaa ggattttggg acagggatta agttccatgc ttgcagatga
      961 ctttgcaaca tttttagtgg aaaagaattt tctccaacaa gaagaattga ttcttgattt
     1021 agtaaaagta ggtggtgcaa agattttaga attaatcaag catacaactg gtattaatcg
     1081 aaatttaaaa aatattaaat atgcgataaa gaatattgtt cgaattgttc gagctcttaa
     1141 atattattcc catttggatc aagcagctta tgcagaggct gacttaattg agggaatcga
     1201 aaatactctc attattgttc acaatcaatt gaaaatggga attcaaattg agcgaaattt
     1261 tcaaccaatc ccacttgttc catgtaacct agatgaatta aatcaagtct ggacaaatat
     1321 tattcaaaat gcaattcatg ctatgaaagg aaaaggaatt ctaaaaatct catcttatct
     1381 tgaagagcct tttgttactg tggaaattca agacaatgga actggaatca aacctgaaat
     1441 tctagataga atttgggatc ctttttttac tacgaaagac caaggtgaag gttctggact
     1501 tggacttggt atcgttaagg gaatcattga aaaacataaa ggaaaaattt ccgcaacatc
     1561 taaaccaggc gagacgattt ttaagattca attaccttat accaattccc aaaagtaaat
     1621 tctgattgga aactagaaaa cagtcttttt gggaattggg aatatccctt ttgtgaaaaa
     1681 tagcagaact tatttatgag aagtggtaga taaaaattaa tcttgactcg aagtcagaaa
     1741 ataatttgga tagacctagt aatcaaaaga ggatcaatct aaatggaacc atttataccg
     1801 gcaagtttta atctagcact caactggact gcagatttat tatttccagt tttactcaaa
     1861 gctgttcaga atattaacga agtaattatt actgatgaag ataaagaaat gcttcgtagt
     1921 ttaaaaaaag aacgtctttt atttttcaca aatcatccaa caaccgcaga accaccgatt
     1981 gcatatcata ttgcaaattt aatgggaact agatttaaat tcatggcatc acgacaagtt
     2041 tttgaatgga atggtggtat ggtagggaaa gttatttctg ggattggagc attttctgtg
     2101 atcgcgggta ttccagatag agactctttg aaagctgcaa gaagtgcgtt agctgctaaa
     2161 gagggaaagt tagttttatt tccggaagga gagccaacga gtggtgagaa tgacaatcta
     2221 atgccatttc agccaggtct tgctcaatta ggattttggg gttatgagga tgctaagaaa
     2281 atagatccta acgctgatat actcgttctt cctggtttca tgaaatacat tgtagatgca
     2341 acagaaaaag agatccgaaa tgatttagaa gaatcagtcc agaaaattga aaaacattat
     2401 aaaattgatc caggtcgtaa gaatatcctg cgtcgctttt taacaatagg tcgttatatg
     2461 gtagaagaag cggaatcaga atataacatc aaggatgggg acaaacatga tttcgactat
     2521 cgcgtaggaa gaattcgtca tgctatcttg gataattgcg ctgataagtt tcaaatcaaa
     2581 ggatataaaa gagatgatga tgcaattcat aagctacgtc atctatttgc tgttatggaa
     2641 atggtaatga tcaaatatcc agatgaaact ttaccgaaac tttccagtaa agagattgaa
     2701 tggtttaagc gtgaatgtgt aaaagctttt gactttattg ttattaaagg agactattta
     2761 gcttctaatc caactcctga gagattttat gaatggcttg cacgttacga gtcttatatt
     2821 tttgggaaaa taccgagggc attgggtgga gaagcatcgc atctaccgag aaaagcatat
     2881 attaccttca aaaagccttt ccgtctttct gaatactatg ctgaatataa gacgaaaaaa
     2941 cgtggagcga tagacgctat gatggctaga ttaagagcag atattcaaga attgctcgat
     3001 tcttctatgt atttatctcg tccgctagta aaaccatttg atacagaaag tttagtttag
     3061 tttattcact caccttgcac acttagtgtg ctaggtgaaa ttagcggctt ggaatgaaaa
     3121 tctcagactt cggagtattt tagaaaataa ttatgcaagg aagcgatgct ctaaagcatg
     3181 tttccttgtt catcacttca caaaccaaca gaagcgttag caaaaccttt ttgatttatt
     3241 tatcatcatt ctcaaatatt cttaaaatca ccgtttattc aaaaatattt ttaaatttac
     3301 ctaacatcag ttatttactc ttccttaata tttaaaggtc attgctgata taaacatttt
     3361 ttcatgtcta gacaaatcgg cactatctgc ataatcaaaa agtggatcgc tagattttct
     3421 atattttact tcaaagcgta tcattgcata ttcatttgga atgtaatcta agttaagaga
     3481 gattgctccc acttcgaaac catttggctt tttacccggc tcttttgaaa atattgcgct
     3541 ttgttttaca actggaacat tggctccttc tctatctaca tatcgttcta gtctgcctga
     3601 tattctccac tctggaaaga gacgataggt gacccataga gttccatcat accatctttt
     3661 ataagcattg gaatgggcag tgtagtaggg ataatttggg ttaatggaac taggaattgc
     3721 aggattatat ttttctgctg ttccacgaat ttgaaatcct atatcaaaag aggctgcaat
     3781 ggaaaaacga tccgttatat cataatggta gataaagttt tggtaatagc gagtatttct
     3841 tgtatatcca agtcccttcg aatacatata gacagttcca attgcatctg ttttcgttgg
     3901 agcagaagtt tcatttccga taaaattgtt ataagtaaat ttcattttat cagttatctt
     3961 atatgctact tgcattccga gagctttatc tttattatta tctgttatat tttgccatcc
     4021 attcataatg tgaagttgaa cggataattt atctgtcact tcatgcgata tacgcacacc
     4081 agttgaatag taaggaacgt tatctagaac gatagcgcgg gtatacaccc aattgtaact
     4141 tgatacccaa gcttcaaaac cgatatttcc catgtagata ccagcatcga tccaggttct
     4201 ctttcctagc tttatgccta cataagcttc ttgtatattt cggacagaat tttggtttga
     4261 atatttttga tttgtcgtct catttgaata atttgcattt acagaagttc cggtttgtaa
     4321 tgctaatcta gctctaaatt tttcagcatc taactttcct tcaatatgtg cgaggttgat
     4381 tgcaaaactg ttgttttgaa ttgcctgagt tgtataattc tgagccgttg cggttggtct
     4441 atttaaatta gcattatagt aaccatctac aaaaactcca aaatcgattg gaagctttgt
     4501 atttagcttc ttatcatcta tttttatttc ttgttccttg tctttaggat tcaataccct
     4561 agtatcaatc gtttgttctg gttggcttga atcgatagtt ttagattgct ttgtttcctc
     4621 ttgtttattg ttgacggttt tggaatcagt gttcgaggat tcttgtgcta gtatagagga
     4681 cacaaataat aaactgggca atattaaaaa aaatttaaac ttaattaaat aaaagatgtt
     4741 tttcatgaat ctcctgttgt ggtttccctc taaattagga ttgagtagat tggaaacttt
     4801 atatatgtat tttgaattac agaattaatt acgaaaaata ttatgcaaat actttcaaaa
     4861 gaaaaaggga tgaatcttta ttttttatcg ataggaaaaa aatatgaaag ccattcaaag
     4921 taatctctat tcaaaataaa tatgcgatag ttagaggaga ggattcgaag taaaggatta
     4981 gtaagaaaaa gatttcattt tgttttaata ttccaactgg aatcgatcta attaaagatc
     5041 gaactttcac ttttataaac gaaaaagaga attagtttgc agtccttgga agcaaacttt
     5101 caatgccttc ttagtcggat aatatggatc atacattttg tttaattaat ctacaattta
     5161 atcttttttt ctaatttcat agatatgaat ggatttgttt ttattaattt catctaccgt
     5221 ttgacgcttt cttctatctg acttaaatcc ttttataact tctagtcctc ttgagcgata
     5281 ttcagatgta tttgtgtttc tatttggttt atgaatcatt tgttctttaa cgtattgatc
     5341 taacttttct tgatttactt ttcctgtagc tgtgactatc ggttctaatt ctgtttcctt
     5401 tggttttgta tcaagagaga aatcatataa aaaactttca ttctgttcta taatattttt
     5461 ttcaatttcg ggttcaagct ccgtcttatt gtttgcttta tagttcttaa tgctttctaa
     5521 aatgatagct tgacgtgctt gatgaatcag ttctgatttt tggcaaacac agggggcaaa
     5581 agtcatttcg tagaaatcat cattcttttc cgtttcgatt ttactcccag tagaagaaca
     5641 ataagtatat aagaggatta ttatataaaa gtaatttctc attcgcataa atataaatgg
     5701 attttggatt aagtcaagat gaaatatgct ttacacactt ttaaatacct agaatttatt
     5761 gttatgcgtt tcctgttctt ttttattttt attatattta atctttttcc tgagtctcca
     5821 agtgttgaag actctgaatt aaaaaaagga aaagtttttt cttcatttga tgatgctttg
     5881 aagaatccag aaaagattta tagactgaat ttgagtaata ctcgaatgaa tgaaatacca
     5941 agtgatattg aaaagtttac ttcattgcag gaacttagtc tctcttctaa taaaatagag
     6001 tttttacctg atcaaatttg caaattaaag aaactccaaa agttaaatct aagttataat
     6061 catttaaaaa tgcttccatc ttgttttgga gaactggatc aattacaaga attaaattta
     6121 acggctaacg aaatgttatc ccttggtcct gaaattggac ttcttaagaa gctagaacat
     6181 ttaacgttac tggagaatac attgcaggaa ttaccgaagg aatttggaaa attggcaaat
     6241 cttcagaatt tagatatacg aaaaaataaa ttaagagagc ttccagagca gatcagtgaa
     6301 ttaaaaagcc ttaaaacttt aaatttagcc tccaatcaga tcacgaagct tccaagcggc
     6361 ataggaggat tatccgctct acggttttta gatttaactt ccaataaaat caccatacta
     6421 cctgaatcaa ttgggttatt aaataattta gaagagttga atcttagttc taataattta
     6481 ggattacttc cttcatcttt tggaaattta tcagagttaa aaaaaataaa tatcgaaaag
     6541 aattcactga tatctcttcc ttcagaaatt tcaaatttaa aaaaacttca aagaatatat
     6601 ttgagtcaca atagtctcga taagtttcca gaaggtttaa cgaatttagg tgaggtttta
     6661 tctatagaaa taaataataa tcggataaca catcttccag attcaattgg atctatgact
     6721 atgcttcaaa ttttaaatct aagctcaaac aaaattactt ccgttccaga atcttttggg
     6781 gatctcaaga gattaacaac attgtatctc aaaaaaaatc ctttttcaaa gccggagcaa
     6841 gaaaaactta aaaagttaat gcctaattgt aaaatttatt gggattaaat acttcggtta
     6901 cgcaagatta gccgctttgt taaaagacgg gctagaaaaa ctccatagta tggatatttt
     6961 ttaaccatat ctataaaacc acttctagag atcggtaata gttgtgcttt cgtcattgct
     7021 ttaactgttc cagttctctt attatgatgc aaaaaagaca tttctcccac aaacatatct
     7081 tctggtgtaa gcactgcgat acgctggttg tttttgatat ataccccgag cttacctgat
     7141 aatatataat agaaataatc actctcggaa ccagattcaa atagaatatc atcaggattt
     7201 aagaaaagta tttcgcctgt cgcaaactgc atgagatgac tcgctctccg tttaatctct
     7261 gtattattat aaaatataag ttttacaata tttcccttgt cattgtattc catttcgtct
     7321 acaaagttac gtgtcattag aattcctcta ccatgaacag cgaaaatatt ctcttcactt
     7381 cgaggacttg gtatagcact tggatcaaat ccattgcctt catctttgat tgtaaatata
     7441 gcttggtcgt tgtttatttc atagctaata gtaacacgtt tatttatatt ctcaggatat
     7501 gatgctcttt cgtctatcgc tccatacata tccatactat ctgttagaat ttttgatttc
     7561 atctcaaaac ctacaccgca gttgccatgt tcaatcgcat tttgaatcat ttcatgaaga
     7621 gagagtgata ttttagaaaa tgcttcataa tttctaaaac cagcagcata acagatactc
     7681 ataattagat ttgcaactct tggaactaag cgaaggttgt ttttgatttt tatttttcct
     7741 ttctttcgtg tgatattttc tagaagaaat tgttctgact caaataattc taaattggaa
     7801 gttacaattt ttaggatcgt tggaatttta agacggactt cattagaggt catgaagtca
     7861 atcactccta tatctaagag ttttacaatt tcagcttcgg ttaatttatc tgcaataatt
     7921 actattactg tcccatgtag ccaagggtca ttatgaactt gatttacagt ttttgcgagg
     7981 aagtcaaatt gacccgtgtg ctcgatgaca aataacctag aagtaatttg cccatatgtt
     8041 aagtaatcaa ttgccttatc gatttggtaa aataccttcg gatgaaaaga tcggacatta
     8101 tcttccgcca tttgtgctgc ggtaataatt tcatcaatta cttcgggagt ggatgcgtag
     8161 acgctaaaag atatattttc ttttttcgtg ttcataactt atttttagca atatttattc
     8221 catcctcaaa agctttcagt gttttttctt cgaactcttt tggatagtca tcccaaattc
     8281 tacaatgccc tgtatttttc gcctcaaaaa aaactttttt tcttatactc aaattggagt
     8341 ataggtctac tccatgctga tacggtgtag ttttgtccgc agttccatga aaaacaacga
     8401 tgggaatttc aggaaaatct cttacaagtt ctcctggttt aatactttta aaatcaaatt
     8461 cacctttaaa actaacgtat ttaaaaatga tctctagata aaaatctggt aagaacttgg
     8521 atattttgtt ttcctttata attctttcca tggagtaaaa tggattttct atgacagctg
     8581 cgtctacctt acctatccag ttttcttttg atagaataaa tgttgatgtt cctgcagaag
     8641 tagaaatcac aactacatgt ttatatttct cttttgccca atttaagact gcaataaatc
     8701 caatcgattc cctaattcca tatgaaactc ctagaccatt gtttgtcgtt cttccatgat
     8761 tcggagcatc gtataaaatt acgttataat cagatttaag tagaaatgga actaatttga
     8821 atccatttcg tctatcggct ccagctccat gtataaaaat aactactcga ttggatttag
     8881 ttgacgcagg aaaaagccaa ccatctatta taaattgatc tgtttgccat tgaatggatt
     8941 caaatttcag ttttaccgca tcctctggat gatcgataca aagctcaggc gaaaattcaa
     9001 gttgagattg ggaacataaa tgtgtcaatc ctggatgatg ccagtgtgga tgtaatatca
     9061 tgtaggaaaa aagaaagatt ccactaatat agagggttaa taggaatgcg attattatag
     9121 caagagtatt tctaatattt cttttcatgc ttaaaataca atttgtttat gagattaatc
     9181 gatattatcc actccgtaaa attagaagag aaaccaatcg attctattag catttagttt
     9241 ttgtgaatgg aatgttgtca agtctgcaaa attatttttc caacagattt gcctgattta
     9301 aaataataga gtgcatcaag cgcttctgta aaagggaaag tttttccaat caatggaggt
     9361 ggtaatttta aatctaaaat ttgttctaac attttgctta gttcatccat tttatcccag
     9421 agccagatca agttgaacgc taagactgat ttgttgctgg atatcataga aagtggatca
     9481 attctaggtc tagttaagta ttgatatgct aatttaaaaa aattgggaga actagattct
     9541 ggagcaaaat gtgcagctcc gtaaactaca attcttccag ttggagagag ggaatcaaaa
     9601 ctgctttgaa atattttccc accaatacat tctaaaacta aatttaaact tctgtctttc
     9661 aatttatttt ttagttgggt tgggaaatta gaatctcgaa taatataatc atcatagcct
     9721 tctgatttca gtgtttgaat tttagaacta tcaccaattg tcccgatcgt atatgcatca
     9781 aacttttttg cgatacgatt tgcataaatg ccaaccccac ccgctgcact gtggataaga
     9841 actgtttgcc ccttttgtaa atttcccaaa gggaatagtg catagtatgc tgtgagagct
     9901 tgtgcaataa atgcagcccc ttcctgataa gaccaattag aaggaagggg atgtatatag
     9961 gatgtatttg aattcaaatg acttacataa ccaccaaagc gggtaacacc cattatccga
    10021 tcgcctatct taaaattttt aacttttgct cctgttttaa ttacatttcc agaatattct
    10081 aatccaggaa taaaactacc tttaggtgtt gcactgtata atccaacgag tgcaaaaaca
    10141 tcagcaaaat taagtccaat tgcttttact tcaattgtta cttcctcttc aagaggatcc
    10201 gagagactct ctgaagataa ttttaagtct tctagagagc ctgctttggg tgtgcgataa
    10261 actagccggt tcattttttc tttttagaaa ctcttggctt ttttaattta gattctactt
    10321 tctcttcggt tgcagtggtg gaatctagtt tctcttcttt tttcttttta gaaggtttct
    10381 tagcttttgt tttcttttgt gcttcctgcg ttagttttac ttttaaatct tctttcaaat
    10441 ttaaatctac ttttacagga ggtttgattt caggcactgt ctcagaatct tcactgtcca
    10501 aatcaattgg agaatctttt aatgcttcta gtttttcttt aactttctct ttgagattgg
    10561 tgtcgacgtt agtaggaaga tctattgatg gagtctctga agtatcatta tctaggtcaa
    10621 tcggagaatc ttttaattct tcttgctttt cattgacttc tactctagat ccatccttat
    10681 tttcaagcgt cgattcatca gatttggaat ctgaatctaa atctattaat tcttctgtgg
    10741 attcatctgt aattgctttt tcttctgctg tatcaacatt taaaccaaga cttaaatcag
    10801 gaatcttttc ttcttctttc tttggtgcat agacaattgc cagcacagat ggaagaagtg
    10861 taagtgctac aagaagagaa gaaccaatac ctatacttgc aactacccca agagaatgta
    10921 aacctctatg ttctgctatg agtaaaaccc cccaaccaac tagagttgta agcgtagatg
    10981 caataattgc aggacctact ctagaaacag ttttccctat attcttttct tccatgaagc
    11041 gatagtatat atagattcca ttttgaattc cataaccgat cacaatagga aatactaata
    11101 cgttgaaaaa atttaccttt gctccaaaga ttcccataat tccaagcatg actgctaatc
    11161 caactacaag tggaaagagg gaaagtattg cagggatgag tcctctataa aaaacaatca
    11221 gaattattaa tacaatggtt agagtcattg caacagccgg atatgcttct ctttgaacga
    11281 tcattgccag tttagcaaat agaattacac ttcctgctgt atgagctgta tctttataac
    11341 ttctcatttg ttcaattgag gta
//
LOCUS       DAOQOS010000148        11103 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_3036_length_11103_cov_7.311731, whole genome shotgun sequence.
ACCESSION   DAOQOS010000148 DAOQOS010000000
VERSION     DAOQOS010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 11103)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11103)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11103
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3036_length_11103_cov_7.311731"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(330..497)
                     /locus_tag="PLF66_22310"
     CDS             complement(330..497)
                     /locus_tag="PLF66_22310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66838.1"
                     /translation="MSGKILFLLGALWVSCAITLLFSYEPIKYFAEPFMMILGIAGFI
                     ISVYEFFSKSK"
     gene            complement(1077..1952)
                     /locus_tag="PLF66_22315"
     CDS             complement(1077..1952)
                     /locus_tag="PLF66_22315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66839.1"
                     /translation="MTTTTNNKEKNHEPTKPKRGKQRFAIREREREKVISPSRSESKS
                     NFSRAILKEWFLREAVKPMLRGGFISIGFVIGIATIGLMAIAVTGTFNTFTSGTVVKA
                     SDINTNFATLKAAIESIPSQPSMRLIYETDLTGTQNNINVTGLDGNTDKEYMIVARIV
                     SPITAACAYGIQPNGITGSSIYGFQYVGAGGPPSAIAGRNTNELFLNMTTNNTAGQVA
                     YANSFMLAKTGASRVLHTLNVQQLAAGTSTIGDNYVFSAVWTDSTTNITSLNFAAQNN
                     SCYGAGSHIEVWARR"
     gene            2506..2967
                     /locus_tag="PLF66_22320"
     CDS             2506..2967
                     /locus_tag="PLF66_22320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66840.1"
                     /translation="MIIFFLGAFITGVGFLMNRMNVMKYKNNGISIPAYITDMETYSQ
                     KISRKGRTREVNMLQLKFTLNGTPGSAHVSEYIGGTEMESYKDGQKVDLVYLPDSVYI
                     ANGKVSFIRPVMLKTTIEFALDRLSWYPYIGGTCLALGLILLVVPRFASKK"
     gene            3517..3891
                     /locus_tag="PLF66_22325"
     CDS             3517..3891
                     /locus_tag="PLF66_22325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66841.1"
                     /translation="MQIPRKKSKLTDENGKYLMLELNVENENVDANHYIPKDPRYPDA
                     GRYLIRSKETSQWLIIEDIAQADATIKQLKQIHGIRDPWDDDPNYFRSVPIPDPTAAF
                     ESLTSEEKDAVQKVLNWRAAQD"
     gene            3946..4098
                     /locus_tag="PLF66_22330"
     CDS             3946..4098
                     /locus_tag="PLF66_22330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66842.1"
                     /translation="MREYPKEYKIESVKLYLANGRQLNFTPFSSKPLFMKDSQSFFDL
                     SHSLVR"
     gene            4439..5017
                     /locus_tag="PLF66_22335"
     CDS             4439..5017
                     /locus_tag="PLF66_22335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020775727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HMZ66843.1"
                     /translation="MKEKSYIKLLDEARAGDLKAWTSLQNRFSNYALYFATKIVKDSD
                     LAQDVAQESFWEIFRNLNKITTSAGFPNLLRKVIMKQSDRILRKKDSKNLPFSEVNLK
                     DKSSKEIHSALVEKDSIDIVIEKVKKLNSIDQNIIELHYYKNLTLNEIAESKGKTLSF
                     VKKRHLQLKKTLRIEIGESFRPQASLHLLKAA"
     gene            5026..5595
                     /locus_tag="PLF66_22340"
     CDS             5026..5595
                     /locus_tag="PLF66_22340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020775734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HMZ66844.1"
                     /translation="METAQLSPHNQIDNLYLEQRKVFLWGEVNDASARHLIDRFLFLD
                     SMDPTKPISLYIHSPGGSTYSGLAILDIMQSIHSPVHTVCLGMAMSFGAVLLLCGSHG
                     NRFVYPHSKVLIHQPHIMGEYKGTAEDIRIFSASVKREKDLLNEIIAKATGQTLQKII
                     QDTNRDSWFSAKEAIEYGIADKIIGNDFK"
     gene            5695..6462
                     /locus_tag="PLF66_22345"
     CDS             5695..6462
                     /locus_tag="PLF66_22345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66845.1"
                     /translation="MILNIKYFLILFLILNTEFIYANEAKVVHVFVALCDNKNQGIVP
                     VPEKLGNGEDLENNLYWGARYGVKSFYKSEKDWKLIHSSLNINEFILERVVFWNHKKK
                     TYLVADAYKGSKIKKSIENLILSVKQIHIDSVQVYNPFFKKEILLQLAANADLLAFVG
                     HNGLMDFTVDLPFKRVENKKSIPIILLACYSKSYFSNQFKSNEIDPILWTNGLMAPEA
                     YVLNAGLSSWLDNKNFTPFSSKHLFMKDSQSFFALSL"
     gene            6527..6673
                     /locus_tag="PLF66_22350"
     CDS             6527..6673
                     /locus_tag="PLF66_22350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66846.1"
                     /translation="MLPLAYGLGVYFQLGSLGLWSAISVWLVCLSIIFVWEFNQGDWK
                     THKI"
     gene            complement(6724..7413)
                     /locus_tag="PLF66_22355"
     CDS             complement(6724..7413)
                     /locus_tag="PLF66_22355"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HMZ66847.1"
                     /translation="MVRDKPSQKGNEIGIFSKNTKVTLLEKTEVIEKIGNFIAEWAMV
                     KNEKVKGYVYGAHLAGNKSLTLCSWDKKKLYIHLYSGKKIKMDSEYYAKEVDAKIYSQ
                     CEYDEFFKGILIQSGGYEGESYQLYDLNTGKDYDICNSEYILSPSKKFVLFFSDDLEA
                     NYNKNGIEIFNNQYPLQKLLNYDFTDSKPCNPKWISDSKLKIKTCDTNGKEIGEKIFI
                     HKNNKWQELRN"
     gene            complement(7577..7900)
                     /locus_tag="PLF66_22360"
     CDS             complement(7577..7900)
                     /locus_tag="PLF66_22360"
                     /inference="COORDINATES: protein motif:HMM:NF026918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 53 family protein"
                     /protein_id="HMZ66848.1"
                     /translation="MQTKTKFQEEIKINNLKWLQTWFKEQCNGDREHSHGISINTIDN
                     PGWKIEISLSETDFENLKQDFIKINNSEDDWYTFGIKDATFIGAGDPFKLEFLIGKFR
                     DLVTK"
     gene            complement(7857..8144)
                     /locus_tag="PLF66_22365"
     CDS             complement(7857..8144)
                     /locus_tag="PLF66_22365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66849.1"
                     /translation="MARVKKLKPDKDALDDHTTFIENLLGKILRYATYFKNKLNPNGF
                     DEVKRVDLFGDEHFNKVTNEFISTPHVHEKGIQGEIRNANEDEIPRRNKNK"
     gene            8702..9082
                     /locus_tag="PLF66_22370"
     CDS             8702..9082
                     /locus_tag="PLF66_22370"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HMZ66850.1"
                     /translation="MGDAVMASFQKNVNSFDAIFSAMKRMKEYNLIKDISESVFLKIG
                     IHIGAAILVNLNDRLDYFGSTVNKAARIQSISKSEQISFSEEVFSDKEVRSILRANGI
                     NRVSKSSRNLKGIEGKYSIYQVAL"
     gene            9329..10849
                     /locus_tag="PLF66_22375"
     CDS             9329..10849
                     /locus_tag="PLF66_22375"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HMZ66851.1"
                     /translation="MKRKILVIVILLSTIVINAFSVLSLEIYNNTLHNSKNWINLKTK
                     VVRGVSGAASFMNTMPALFKFKLNLATWQGHHKLIFTQELQPKEISFKYKVDKEQFFS
                     FLFNSDEKGIKSYGIRISNSAFYPSMYFQIENEKFTNKLPLTNFKSDDKNHLFKIKFD
                     NEAFTIFIDNQEIFRATDSISAKMHIGFKSGLTDLRTDIDDLIMIQQDGTRLEETFFN
                     WKLIPELTLYCLAVTILVFGLLYFLLKRRFQTDTMPILKILSYVNFNLLILSLFVYAA
                     MYSTVSRIYFEIPKVYNGYPTIKQTEEVVSEFVSQYGNNKNKPRIWFIGSSQTWGSGV
                     YREEDTFVRIIEKELQKAGYNIECINGGVSGFTSAMALDVFNANFDKFKPDYVILNLS
                     HNDAFFKISPDEFIKNLRAFVEKNRVHHVTTILVVEPVETHFLESKKHQDIMREFAVK
                     ENIPLIDVHNELVLKGDDGFLWWDRVHLTSYGNLLFAQVMLDPLKKIINTQRNFLY"
BASE COUNT         3674 a         1797 c         1837 g         3795 t
ORIGIN      
        1 tttagttcaa aacaacttat tatgaaagac tcgcaatcct tttttgcttt aagccattca
       61 cttgtaagat agaaatggga gacaattatg ggagaagttc gatgcaaatt cctagaaaga
      121 catgcttgga tgctgctgct cagggtgaca attttttgac ttcgcatctt tgtgtgaaaa
      181 aatccttcta aggtaattcg gtaggctaaa cgatattact ctctctctct ctctaatggc
      241 aaatctttgt ttgcctcgtt ttgttgcttc attggctttt cctttgtatg attttaaaat
      301 attattttga ctagagttta agctttagat tattttgatt ttgaaaaaaa ctcataaaca
      361 gaaatgataa atcctgctat tcccaaaatc atcatgaatg gttcagcaaa atacttgatt
      421 ggttcgtaag aaaacaatag agtgattgcg catgaaaccc ataatgctcc caataggaaa
      481 agaattttgc cactcataaa atcatcttct agtcgcgatt tcttctaaaa ttctgtgatc
      541 ttctttttgc tttctaccta aatctttaac ttcctcttcg atattacgaa tttcatctcg
      601 aaacattttt tgaattccgc ggtacatcat gaacaatcca gaagctaaag ttaagattga
      661 aaataaaata aaagtcaggc tcatatttct aactgtttaa gtgttttgat tgaaagtcaa
      721 cctattattc acaaagattt ttacaaaaat tataggacaa atgatttttc attttgaatc
      781 acaggcaaca tgcgatttgt ctggaagtat tagagttgtt gataaattaa ataacgtgaa
      841 ttcgatgtaa gcgcggtttc actttgtcac ccccgaattt agttatcggt ggactctcga
      901 actagagatt cccgacatcg cttggcggta gcggcaaagc gggtggaatt tcaggaatga
      961 cagtctaaat aatttgtcaa atccttatat cgagctcacg ttaaattacc aagatagtta
     1021 ttataaatat tctacagcct ataatagtcc gatttggtca ttccgccaaa tagccattat
     1081 ctcctagccc atacttcgat atgagagcct gcgccgtaac aactattatt ttgcgcagca
     1141 aaattgagtg atgttatatt tgtagtcgaa tccgtccaga cagcggaaaa tacatagtta
     1201 tccccaattg tagatgttcc tgctgcaagc tgttgcacat ttaatgtatg tagaacacga
     1261 ctagctccag tcttagctag cataaatgaa tttgcatagg ctacttgccc agccgtatta
     1321 ttcgtagtca tatttaagaa caattcattt gtgttccgcc ctgctatcgc tgacggggga
     1381 ccacctgcac ctacatattg gaatccataa atactacttc cagtaattcc gtttggttgt
     1441 attccatatg cgcaggcagc cgtaattggt gagacaattc ttgcaacaat catatactcc
     1501 ttatccgtat ttccgtctaa gccagtaaca tttatattgt tttgtgttcc agttaaatca
     1561 gtctcataaa tcaaccgcat actaggctgc gacggtatac tctcaatagc cgcctttagt
     1621 gttgcgaagt ttgtatttat gtcagaggct tttacaacag ttccacttgt gaatgtatta
     1681 aaagttccag ttacggctat tgccattaga ccgatagttg caataccgat tacaaaacca
     1741 atgctgatga atccgcctct taacattggc ttcactgctt ctctaagaaa ccattctttt
     1801 aaaattgctc ttgaaaaatt acttttgctt tccgagcgac taggcgatat tactttctct
     1861 ctctctctct ctctaatggc aaatctttgt ttgcctcgtt ttggttttgt gggctcatga
     1921 tttttttcct tattattggt ggttgtcgtc aaggttagat tgagtcaaac cgaagtcaag
     1981 cattgcatac aggttcattc cctgaattcg aaatttcatt ctctaatttt gtgaaaacca
     2041 aaaaaataat atactgacaa ttcctttcct tataaaagaa tgaattctag taactcatct
     2101 tgcacacagt gcataagctg agttacaaaa aaaaataggt taggaataaa aatgaattat
     2161 ccgctaaaac tttcttttaa attattcgcg cttgctcctc aaattaaggt ggcagataac
     2221 gcaggaaaaa ttttaccccg tttagttcaa aacaacttat tatgaaagac tcgcaatcct
     2281 tttttgcttt aagccattca cttgtaagat agaaatggga gacaattatg ggagaagttc
     2341 gatgcaaatt cctagaaaga taatattgat tatcttactg agatttcgaa ttttaattta
     2401 agaaaaaata atcgccatac ttatttgaaa taatagcata gacaggatac aaatttaaaa
     2461 ataggaagaa caaatagaat ggcaaaactt ataagaatat catttatgat aatttttttt
     2521 cttggagcct ttatcacagg tgttggattt ttaatgaacc ggatgaatgt tatgaaatat
     2581 aaaaacaatg gaatctcaat tcctgcatat attacagata tggagactta ctcacaaaaa
     2641 attagccgaa aggggagaac tcgagaagta aatatgctcc aattaaaatt tactttaaat
     2701 gggacaccgg gctcagctca tgtaagtgaa tacatcggcg gaacagaaat ggaatcatat
     2761 aaagatggac agaaagttga tttagtttat ttgccggata gtgtatacat agcgaatggc
     2821 aaagtgagtt ttataagacc tgtaatgtta aagactacta tcgagtttgc gctagatcga
     2881 ttgtcatggt atccttatat tggcggaaca tgtttagctt tgggattaat tctattagtc
     2941 gttcctagat ttgcttcaaa aaaataactt aaaaatgaaa ataggcaaat aaactttttc
     3001 caactgtcac tcccattatg ttccacccca cgaagcaacg cttgcgctat tgagtttcat
     3061 tttttatcgg gagtatgcaa aacgctatgt tctagataca cccgattact aaactcggag
     3121 tgacagcttt cggaaaaata ctatccctat gaaattactt aggaaggaca atttaaaatt
     3181 atccatcgct ttttatcatt gagttatcgg tttcctcagt ggtattcttt ttttgtattt
     3241 cttattttaa taaaaattcc ataacatcat ccattacttc atttcgcaca acgtctcgct
     3301 ctaaaaatat ttcatgtttg gaatctttaa caaatctgat tttacaaaga ttttgtttgt
     3361 tcttattgat cgcatcataa acggtatttt gaccagatgg ataaactcta tcccaattat
     3421 ttcaaaacat ctcattctaa aagcctcgct atcctttttt gctttaaacc tttaacttgt
     3481 aggatagaaa cgggagagaa taatgggaga agttcgatgc aaattcctag aaagaaatcg
     3541 aaactcacgg acgagaatgg aaaatacttg atgcttgagc taaacgttga gaacgaaaat
     3601 gtagatgcga accattatat cccgaaagat ccacggtacc cggatgccgg gcggtatctg
     3661 atacgttcaa aagaaacgag tcaatggctg atcattgaag atatagcaca agctgacgcc
     3721 actattaaac aattgaagca gattcacggg attcgcgatc cttgggatga cgatccaaat
     3781 tacttccgca gtgtaccgat accagaccca actgctgctt ttgaaagttt gacgagcgaa
     3841 gaaaaggatg ccgtccaaaa agtactgaat tggcgcgctg cccaagacta gtgaacccct
     3901 acataaaaaa aggacacgat tttaggcttc caaaaggaga ctaaaatgag agaatatccc
     3961 aaagaataca aaatagaatc agtaaaacta tatctagcaa atggtagaca attaaatttt
     4021 accccgttta gttcaaaacc tctctttatg aaagactcgc aatccttttt tgatttaagc
     4081 cattcacttg taagatagaa acgggagaga ataatgggag aagttcgatg caaattccta
     4141 ataaaacaac tagaataaaa atagaaatgc tctgcgaaat tttcaagaaa gacggcacca
     4201 aagtcctaga aggtaggagc aaaggtaagg tcttatctaa taccatatag aaaattttgc
     4261 tccgtttagt attttatttc tctggaaata caaaatcctt tcttaaaata tataacaata
     4321 aattaaaagg attctattct aatttctgat ggagtaatga ctgataattt tcaataaaaa
     4381 tatgggatag aaattttttt tggtgtccaa tttttgaatc aatgggtcat ttaattatat
     4441 gaaggaaaaa tcttacatta aacttttaga cgaggcaaga gctggtgatt taaaagcctg
     4501 gacctctttg caaaatcgtt tctcaaatta tgccttgtat ttcgctacta aaattgtaaa
     4561 ggattcggat ctggctcaag atgtagcaca agaatctttt tgggaaattt ttagaaatct
     4621 aaataaaatt accacatcag ctggttttcc caatttgctc aggaaagtta taatgaaaca
     4681 aagtgataga attcttagga aaaaggactc taaaaatcta cctttttcgg aagtgaacct
     4741 caaggacaaa agttctaaag aaattcattc agccttggta gagaaagatt ctattgatat
     4801 agttatcgaa aaagtaaaaa agctaaattc aatagaccaa aatatcattg agttacatta
     4861 ttacaaaaat ctaacgttaa acgaaattgc tgaatcaaaa ggtaaaactc tatcgtttgt
     4921 aaaaaaaaga catcttcaat taaagaaaac attaagaatc gaaatcggag aatcatttag
     4981 accacaagct tctcttcatt tactaaaagc tgcttagagg aaaatatgga aactgcgcaa
     5041 ctatctccac acaaccaaat tgataattta tatttagaac aaagaaaggt atttctttgg
     5101 ggtgaagtaa atgacgcatc agctaggcat ttaattgata gatttttatt tttagattcg
     5161 atggatccaa caaaaccaat ctctttgtat atccattctc cgggcggctc cacttattca
     5221 ggtttggcta ttctggatat catgcaaagc attcattcac cagtacatac agtttgtttg
     5281 ggtatggcaa tgtcgtttgg tgcagtatta cttttgtgcg gatctcatgg taatcgtttt
     5341 gtttacccac acagtaaagt tctaattcat cagccccata taatgggtga atacaaaggc
     5401 actgcagaag atattagaat tttttctgct tccgtgaaac gggaaaaaga tttactaaat
     5461 gaaatcattg caaaggcaac gggtcagacg ttacaaaaaa ttattcaaga tacaaatcga
     5521 gattcttggt tttctgctaa agaagccatt gaatacggca ttgctgataa aataatagga
     5581 aatgatttta aataaaaagt ttatcttttg attcaatgta ttatccgctt accgattgat
     5641 aaaataaaaa acatttcctt tacaaaaata aacatcacct ttacccagaa actcgtgatc
     5701 ttaaatatta aatatttttt aatactcttt cttattttaa atacagaatt catttatgca
     5761 aatgaagcaa aagttgttca tgtatttgta gctctttgtg acaataaaaa tcaaggcatt
     5821 gttcctgttc cagaaaaatt aggaaatgga gaagacttag aaaataatct atattggggc
     5881 gcaagatatg gagtaaaatc attttataag tcggagaagg attggaaatt aattcatagt
     5941 tccctgaata taaatgagtt tatattagag agagttgtat tttggaatca taaaaaaaag
     6001 acttacctag ttgcagatgc gtataaagga agtaaaataa aaaagtccat tgagaattta
     6061 atactatctg taaaacaaat tcatattgat tcagttcagg tttataatcc attttttaaa
     6121 aaagaaattc tattacaatt agcggctaat gctgacttgc ttgcatttgt ggggcataac
     6181 ggtttaatgg attttactgt tgatttacct ttcaagagag tagagaataa aaaatcaatt
     6241 ccaatcattc tactcgcctg ttattctaaa tcctattttt cgaatcaatt taaatcaaat
     6301 gaaattgatc ctattctatg gacaaatggg ctcatggctc ctgaagcata tgtgttaaat
     6361 gcaggacttt ctagttggct cgataataaa aattttaccc cgtttagttc aaaacatcta
     6421 tttatgaaag actcgcaatc cttttttgct ttaagccttt aacttgtaag atagaaacgg
     6481 gagagaataa tgggagaagt tcgatgcaaa ttcctagaaa gatatcatgc ttccacttgc
     6541 atacggactt ggcgtttact ttcaattagg ctcacttgga ctttggtctg ctatttcagt
     6601 ctggctagtt tgtctttcaa ttatcttcgt ttgggagttt aatcagggag actggaaaac
     6661 acataagatt tgagtttaat ggttaatgga atatcgtttg cactttggga tttgtattag
     6721 cttttagttt cttagctctt gccatttatt atttttatga atgaaaattt tttctccaat
     6781 ttcttttcca tttgtatcgc aggtttttat ttttaatttg gaatcagaaa tccattttgg
     6841 gttacagggt ttactatctg taaaatcata attcaataat ttttgtaatg gatattgatt
     6901 attaaatatc tcaattccgt ttttattata attagcttcg agatcatctg aaaaaaagag
     6961 aacaaatttt ttggaaggag aaagaatata ttcagaatta caaatatcgt aatcttttcc
     7021 tgtatttaaa tcatatagct ggtaactttc gccttcgtat cctccacttt gtatcagtat
     7081 tcctttgaag aattcatcgt attcacattg ggaatatatt tttgcatcta cttctttagc
     7141 ataatattca gaatccattt ttattttttt tccagaatag agatgaatgt ataatttttt
     7201 tttatcccaa gaacaaagag ttaacgactt gttacccgcc aaatgagctc cataaacata
     7261 accttttact ttctcatttt ttaccatcgc ccattcagca ataaagtttc ctattttttc
     7321 aatgacttca gttttttcta agagagttac ttttgtattc tttgaaaata ttcctatttc
     7381 atttcctttt tgggaaggtt tgtctcgaac aattaattgg ttggcttgaa ctatgtaagt
     7441 ttcatttgct aaaatttcaa atgaaaaaaa ataacaaata aatataggta gattttaatt
     7501 ttttcaaaat tcattttatt aagttatctg taatgctttc cagatgtcta atttagtttg
     7561 cattgaaatt gataatctac ttggtcacta aatctcgaaa tttacctatt aaaaattcta
     7621 atttaaatgg atcacctgct cctataaagg ttgcatcttt aattccaaag gtataccaat
     7681 catcctctga attatttatt ttaatgaaat cctgtttaag attttcaaaa tcagtttctg
     7741 aaagactaat ctctattttc cagcctggat tatctatggt attgatggaa attccatgtg
     7801 aatgttcacg gtcaccattg cattgctctt taaaccaagt ctgtaaccac tttaggttat
     7861 ttatttttat ttcttcttgg aatttcgtct tcgtttgcat ttctgatttc tccctgtata
     7921 cctttttcat gaacatgtgg agttgaaata aattcgtttg taactttatt aaaatgctca
     7981 tctccaaata aatcaactct ttttacttca tcaaaaccat ttggattcag tttattttta
     8041 aaataagtgg catagcgtaa gattttgcca agtaagtttt caataaaagt cgtatgatcg
     8101 tctaaagcat ctttgtcggg ttttagtttt ttaactcttg ccatactact aaagaaataa
     8161 atagctatat gtatgacaag tattattatt aaaaagaaat ctgtttctag tttagctatt
     8221 ttttttttgc acaggtttcg tcatctagct catttttaaa gcttgatata taagagaaat
     8281 attttcttag taaactaatc taaaatagaa ttgttgaaaa atttaggata aatgaaaatg
     8341 tctcttggag tatctcgact tcactcgata taaacttaag tcgagaagtg ctaatataat
     8401 aaagcttttc cacttagtag ttctcgacta tcgctcgaac tgacatttaa tttttaacaa
     8461 tatcaatgaa tgaaaggaat aactcgatga agaaaaattt tacattatat gggctaatga
     8521 ttgttaactt ttccatgatt caggcaaaat tttaccccat ttttcaaaac atctatttat
     8581 gaaagactcg caatcctttt ttgatttaag tctttaactt gtaagataga atgggagatg
     8641 attatgggag aagttcgatg caaattccta gaaagacaag gaagggatta ttgtataaac
     8701 cattggtgac gctgtcatgg cttcttttca gaaaaatgta aattcttttg atgctatctt
     8761 ctctgccatg aagcgtatga aggaatacaa tttgattaag gatatctcag aatcagtttt
     8821 tttaaaaata ggaatacata taggagccgc cattttagta aacctaaatg ataggcttga
     8881 ctattttggt tccacggtta ataaagccgc aagaattcaa tccatctcga aaagcgaaca
     8941 aatttctttt tcggaagaag tcttctctga taaagaagtt cgctctatcc ttcgcgcaaa
     9001 cggtattaat agagtatcga aatcttctcg taatctaaaa ggcatcgaag gtaagtattc
     9061 tatttatcaa gttgcacttt agatatttag aaatttcttc atttctcagg aattaaaaac
     9121 taacaagttt tacactcgat tagaggatac ttctaaatta ccgcgactac ggtgaaaaaa
     9181 acacgctatc taattttgtt tctctttgga cttatccgcc atttcattaa atcgcttcta
     9241 agattttttg tattgatttt atccttactc ttagttttta tgtttttact cggttcatca
     9301 ccgcttcaag cggcaattgg tttttagtgt gaaaagaaaa attttagtca ttgtcatttt
     9361 attatctaca attgtaatta atgcatttag tgttctttcg ttagaaatat acaacaacac
     9421 tttgcacaat tcaaaaaatt ggattaatct taaaaccaaa gttgtgcgcg gtgtttcggg
     9481 tgcggcttcc tttatgaata cgatgccagc gcttttcaag tttaaactaa acttagcaac
     9541 atggcaggga caccataaat taatttttac tcaggaacta cagcctaaag aaataagttt
     9601 taaatacaaa gttgataagg agcaattttt ttcttttttg tttaattcag atgaaaaagg
     9661 aataaagtct tacgggatta gaattagcaa ttctgcgttt tatccttcga tgtattttca
     9721 aattgaaaat gaaaagttta caaataaact tccattgact aattttaaat cagatgataa
     9781 aaaccatcta tttaaaatca aatttgataa tgaagctttt acgattttta tcgataatca
     9841 agaaattttt agggctacag attcgatttc agcgaaaatg catattggat ttaagtctgg
     9901 attaactgat ctaagaaccg atattgatga tttgataatg attcaacaag atggaactag
     9961 attagaggaa acttttttca attggaaatt aattccagag cttactctat attgtcttgc
    10021 tgtaactatc ttggtgttcg gtcttttgta tttcttattg aagcgaagat ttcaaacgga
    10081 tactatgcca attttaaaga ttctgtcgta tgtaaatttt aatttattga tactttcttt
    10141 atttgtatat gcggcgatgt attctactgt atcgagaata tattttgaaa ttccaaaggt
    10201 atataatggt tatcctacaa taaagcaaac ggaagaagta gtatctgaat ttgtaagtca
    10261 gtatgggaat aataagaata agcccagaat ctggttcatt gggtcctctc agacttgggg
    10321 ttccggagtt tacagagagg aagatacatt tgtaagaatc atagaaaaag aacttcagaa
    10381 agcgggttat aatatagaat gcataaatgg aggagtgtca ggatttactt ctgcgatggc
    10441 tttagatgtg tttaatgcaa actttgataa atttaagcct gactatgtga tccttaacct
    10501 ttctcataat gatgcattct tcaaaatttc tccagacgag tttattaaaa atttgagagc
    10561 atttgttgaa aaaaatagag ttcatcatgt tactacaata ttagttgtgg aaccagtaga
    10621 gacacatttt ttagaatcta aaaagcatca agatataatg agagagtttg cggttaagga
    10681 gaatattcct ctgatagatg ttcacaatga gttagttcta aagggagatg atggattttt
    10741 gtggtgggat agagttcatc ttactagcta tggcaattta ctatttgccc aagtcatgct
    10801 agatccgctt aaaaaaataa tcaatactca gagaaatttt ctttactaac acatgatatt
    10861 ccttcgcaga aagaagatac tgaccgtttc gaatggcgga aatctctaat acagatgtca
    10921 tagctgaatc aaaaagctaa tctcctcttt tatcaccgct ctttcatttt tctttctcat
    10981 ctaaattttc gataacaaag ttaaatagta attctatcat tttattaaaa tgttcgggct
    11041 ttgaaaatct atgatcagtc tgttgcgaaa tgttaaagaa ttaaaagtca gcctttaaaa
    11101 ata
//
LOCUS       DAOQOS010000149        11034 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_3063_length_11034_cov_6.087440, whole genome shotgun sequence.
ACCESSION   DAOQOS010000149 DAOQOS010000000
VERSION     DAOQOS010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 11034)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11034)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11034
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3063_length_11034_cov_6.087440"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            249..1691
                     /locus_tag="PLF66_22380"
     CDS             249..1691
                     /locus_tag="PLF66_22380"
                     /inference="COORDINATES: protein motif:HMM:NF019285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HMZ66852.1"
                     /translation="MIDIRLNIISLVGMYVLCIVAWLSSENRKLIPWNVIKWGIGIQF
                     VMGFFIFVFPITRSGVEIFGNAINFLLEASEAGARFLFSGVFVPPVDKVMVKPTDLIN
                     PQTGIVDYSYIVNPKTGFFERSRLDVGFIFAFRSLPQVIFFSAIITLLYRMNAIQPVV
                     NFFAKIFQRTLGTSGAESLSGAANIFVGIESVVAVKPFLLNMTRSELCAILASCFGSI
                     SSTVLGMYAGFLRPTFPTITGHLMAASFLTIPACFVISKIIIPESGMPETMGGVPEDK
                     EDPNEKKPSYMDSLILGALDGVKMAVGIAAVIIAILGCVALVNMLFAWLASFQNNENT
                     LLQFIGRVFKVVSLDNIFGLIFLPLTFFTGVSLDMNELWQSSVLIGQRLLQTSIPPYV
                     KLGELSRAGEISDRAMLIVSYVLCGFAHIPSIGIFVGGLSNLVPSRSGEISSIAWKAM
                     WAATLATLMTGCVAGIFDFGSPDVIGMKPK"
     gene            1851..3107
                     /locus_tag="PLF66_22385"
     CDS             1851..3107
                     /locus_tag="PLF66_22385"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HMZ66853.1"
                     /translation="MKKVILLILILSLLHCKKEVEKPKNEIQFLFTDSLTGTNLYENP
                     EPDSNIISSIPHATKVEILPENPAYTITKENTIKWRKIKFDTFVGWVMDNLLSAKVEK
                     HYFSVLTNAGLPLREKPDVKSNKIATIPFGYIGEFYNKTKENVILDKQPGFWIETTYN
                     GKKGWIFSGSAAISKNLQSLEERNGEFGEEGWFYRFYSNLEESKLEEVEFDLKNIENT
                     AKITQFENHPYTIYQIKRQVAEDDCSGKESQIIFKNNITGKSFGNREIFSETVVEQNY
                     PLVRSVYTTSIPQKCGCLIQDSTLYFLLDDRVLATTFHNKDTKAYCEYGTVSNIELER
                     ENRYDLANNTLYMYLKLPDCQPDEKTILYGGKITNIKKFKTDMFVSLKLSNTEVMIEK
                     FYQKGIPEQFKTAWESSVKEPNLVKE"
     gene            3094..3585
                     /locus_tag="PLF66_22390"
     CDS             3094..3585
                     /locus_tag="PLF66_22390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66854.1"
                     /translation="MSKNRIGIDIGGVIIAEDTDKPDLFFSSHFLKAKPVSNSFETIK
                     ELIDTFSSENIFIISKCGPEIQKKSLLWLEDNFFFQKTGFKRENINFCLHRNEKAIIA
                     ENLNLTHFIDDRYTVLKHFLPLSQIKQLFLFNPNVEEMRLMGSIPSNKITMISSWVEI
                     KIE"
     gene            complement(3609..4658)
                     /gene="lpxD"
                     /locus_tag="PLF66_22395"
     CDS             complement(3609..4658)
                     /gene="lpxD"
                     /locus_tag="PLF66_22395"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HMZ66855.1"
                     /translation="MFSLSDIQRVLPNSEIINCDNPSSVLIAKPVAVPDQGEDAILFL
                     GSKSYLKEVLPLKLGAIVTTKELSVHFKIPVVVVDNPDFALTPILNLFFPPKKSSGKR
                     GQYVSIDSTATIGENTEIGNFVTIEKNVKIGKNCILADGARILEGAEIGDDSVIGANT
                     VIYHGVKIGKRFLCFSNSAIGGEGFRFVPDKKGIFHHVPQVGTVVIGDDVRVGSSCTI
                     DRGGIGDTTIGDGTKIDNQVHVAHNCKIGKNCILAGKSALAGSVVLEDSVIVGGGVSA
                     VDHVHICSGAVFGAGTGVRADVTKPGLYAGWDVFASIVDFQKFRANIKYLPILNQIFK
                     RMKNVEEKLGIESKN"
     gene            4796..6100
                     /gene="murA"
                     /locus_tag="PLF66_22400"
     CDS             4796..6100
                     /gene="murA"
                     /locus_tag="PLF66_22400"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004782508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HMZ66856.1"
                     /translation="MSSSYFKIIGKNPLSGSITPQGNKNEALPVLGAVCMIQGKVRIE
                     NVPLISDVLMLIDVLRYLGFKIENPEPTVFIFERPSELKSDLPQELCSKIRGAVTLAG
                     PILASTGRVFLPRPGGDKIGRRRLDTHLLALESLGADIEVFPDGYEIKANKLIGEDIL
                     LDEASVTGTENAVMAASLAEGTTIIRHAASEPHVQGLCRLLNAAGAKISGIGSNILTI
                     EGVESLKSPEGKEIVHRIGSDYLEVGSFISLAAVTGGELFINDVNPDDMRMIRLVYSK
                     LGIEIRFTDGGVLVPAGQKLEIIPDYHGATPKIDDSPWPGFPADMTSVALVTATQCKG
                     TVMIHEKMFESRLFFVDNIISMGAQIILCDPHRAVVIGKSQLYGGKVASPDIRAGMAM
                     IIAALCAEGTSYIHNIIQVDRGFQDIDTRLRSIGAQIERITE"
     gene            6123..6719
                     /locus_tag="PLF66_22405"
     CDS             6123..6719
                     /locus_tag="PLF66_22405"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HMZ66857.1"
                     /translation="MKLNQFSFKILFSVLIQILWMTSALIADASFDLIQGVKNRKLNQ
                     VSNALNMNADPNIKVNGNRPILLIAAENGDFEIIQMLLAKGAQVNSFETTKENPGWTA
                     LMHSTENGRMDIIRLLLAKGANKKQKNGKGYDAIYLAKLWGNSEVAAFLEGRDINEKK
                     LQPCGKILTEKNLNQKNISLVDLKNNKFYKKSFLSRST"
     gene            6759..7637
                     /locus_tag="PLF66_22410"
     CDS             6759..7637
                     /locus_tag="PLF66_22410"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HMZ66858.1"
                     /translation="MKYIFVSLIGFFLLLESCNTVTTGVSEAVVRPPSLPSSVSFAGE
                     KVPLDDQDVLERLDRELVVNQNYHSSTILIYKNVKRYKEPIERILKENGIPEDFFYLA
                     VAESALNPNATSSANAVGIWQFIQATGEGYGLEVNNFVDERRHFVKSTRAACKYLLDA
                     YKEFGNWTLVAAAYNRGLKGMKDAVTSQKTNNYYKLYLNQETARYVFRIIALKLILSN
                     PQDFNFDLTESMQYPAYKLKSIKVTETIASLPEFAISNGTTYKELILHNPWIRTGKYN
                     FDVATGKSYDFLIPDN"
     gene            complement(7634..8749)
                     /locus_tag="PLF66_22415"
     CDS             complement(7634..8749)
                     /locus_tag="PLF66_22415"
                     /inference="COORDINATES: protein motif:HMM:NF024686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitobiase/beta-hexosaminidase C-terminal
                     domain-containing protein"
                     /protein_id="HMZ66859.1"
                     /translation="MKRIFLISSFLLLIILQFENCKNASTCNQLDSSCNPISSIVLER
                     AIRNSRYGLTFSPPGGTYKYDPFVTITSNSTATIRYTLDGSEPSCTNGTIYTAPAPIL
                     GAKSQTLRAAACVDSVVAATKTDQYTVTLSPLAASNLRFWYSADSIGNIPGLEVKVWN
                     DYSGNGNHLYPSSTGTSSPTIVANALNGKAAVRLTSTQKQFFNSRSATGYIGSKAGVG
                     AMVLRKYSSQTDEVYFSIGFLGNVPNARNWKANTTNQSICSSLSPAPTNCAGAATTAI
                     PTTGYNMILFSTDASGVTTYYYNGVADGTGTIATPTAFSSANVFLLGTGLNVVNYLDA
                     EILEVIFFETGFDAGGINTIHCYIQNKYGLAVGSNCT"
     gene            complement(8782..9132)
                     /locus_tag="PLF66_22420"
     CDS             complement(8782..9132)
                     /locus_tag="PLF66_22420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66860.1"
                     /translation="MIDKGKFLFKANRYKDYFVGFEYLGKHMLGILGYASEREICIVH
                     KGMHVSKTTQDIKGYMKKRGQAEKYEFHGKTTSIENQTHKGRHYTFIWLYFDPPINLS
                     ETLIALEIASFSDI"
     gene            complement(9129..9551)
                     /locus_tag="PLF66_22425"
     CDS             complement(9129..9551)
                     /locus_tag="PLF66_22425"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HMZ66861.1"
                     /translation="MIKKTSLDEFPILENLEKKCFEEEAWNISQIESHHLLNSSILYF
                     DNDLKPTGYIIYLENPFEVEILRIGVLIESRRQGVAQKLLEEVFTISRNREIILEVKS
                     ENSPAIELYKKKDFIIFAVRKKYYPDGKDAILMKRHNI"
     gene            9849..10718
                     /locus_tag="PLF66_22430"
     CDS             9849..10718
                     /locus_tag="PLF66_22430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016547157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66862.1"
                     /translation="MKYFLFTLILVLFSCDKLKEIGQENFKKNVTPKTPSEAELEKWK
                     EKLALDEAEIKELDEKIRKMVKKTKEAGVLSWRIAQGYMRVGNYELSSRYYAQAIQQN
                     MENKVDVVGSEVHLFEAAIPFFEKALLYRELTDDLLFEAGLAYANASRDRGWDKERRN
                     IAVTIFKGLIRRDPADLRYPYELALIYFDSSISDGGIDGVDPAGYNDTEKAMRIMNAI
                     IQKEPNNIPARFARANFLYRSGSTEQATDEYLTIKSKLEAMKEAGVIKESLDKNTSYQ
                     NVVRNLKKINSGK"
     gene            10720..>11034
                     /locus_tag="PLF66_22435"
     CDS             10720..>11034
                     /locus_tag="PLF66_22435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66863.1"
                     /translation="MEELIPFYFSFQSYSSIAHRYNLLTEVSSLFELERKISPILKSE
                     EISKIKLASAQLKKENEKRGIQTIDYFHPSYPTLLKQIDKPPIVFFCLGNTNLLQQDL
                     VAV"
BASE COUNT         3752 a         1911 c         1909 g         3462 t
ORIGIN      
        1 gatgttaatt ttttatttta tcagaaaaag tccaaatttt tatctatttt aaattcaatt
       61 cttcaggaat attcaatata gacagtcaat tttttattga cacattgaaa attaaatatt
      121 tttcatttta atgggattca aaactgataa attctgtgat tctttgcttt aaatttattc
      181 aaaggatttc tcttgacgat aatttaaatt agaaaattgc taatctgaag accgaatcgg
      241 gaaaaaatat gatagatata agactcaata taatctcctt agttgggatg tatgtgctat
      301 gtatagttgc atggctcagc tctgaaaata gaaaactaat tccttggaat gtaattaagt
      361 ggggaatagg aattcaattt gttatgggat tctttatctt cgttttccca attacaagat
      421 ccggtgtaga aatttttgga aatgcaatca actttttact agaagcatca gaagctggag
      481 ctcgcttttt attcagcggt gtttttgttc ctcctgttga taaagtcatg gtaaagccaa
      541 ccgatttaat caatccacaa actggaatcg tggattattc ttacatagta aatccaaaaa
      601 ccggtttttt cgaaagaagt agattagatg ttggttttat attcgccttc agatcccttc
      661 ctcaggtgat cttcttctca gccattatta ctcttctata tagaatgaat gcaattcaac
      721 ccgttgtaaa tttctttgcg aaaatttttc agagaactct tggcacaagt ggggcggaat
      781 ctttatcagg tgctgcaaac atttttgttg gaattgaatc agttgtggca gtgaaacctt
      841 ttttactcaa tatgactcga agtgaattat gtgcaattct tgcaagttgc tttggttcta
      901 tctcttctac tgttcttgga atgtatgcag gttttctacg tcccaccttt ccaaccatca
      961 caggccattt aatggcagcg tcctttttaa cgatccctgc ttgctttgtc atatcgaaaa
     1021 tcatcattcc tgaatctggg atgccagaaa ctatgggtgg agttccagaa gacaaagaag
     1081 atccaaatga aaaaaaacca agctatatgg attcacttat ccttggagca cttgatggcg
     1141 ttaaaatggc cgtcggaatt gcagcagtta tcattgcaat actcggttgc gttgcgttag
     1201 taaacatgct atttgcttgg cttgcatcct ttcaaaacaa tgaaaacaca ttacttcaat
     1261 ttattggaag agtatttaaa gtcgtttcac ttgataatat ctttggctta atctttttac
     1321 ctcttacatt ctttacgggt gtatcattag atatgaatga actctggcaa tcttctgtgt
     1381 taatcggtca gagactttta caaacatcaa ttcctcccta tgttaaactt ggtgaattat
     1441 ctagagctgg tgaaatttct gatagagcaa tgttgattgt aagttatgtt ttatgtgggt
     1501 ttgctcacat tccatcgatt ggaatatttg ttggaggact ttccaattta gttccatccc
     1561 gaagtggaga aattagttct attgcttgga aagcaatgtg ggctgcaacg ttagctactc
     1621 taatgactgg atgtgttgct ggcatctttg acttcggtag ccctgacgtg attggaatga
     1681 aaccaaaata aaaaaagctt gctaaggtgg aagcggaaat gttctactta ttgtatagca
     1741 tttcctctac tgaatgataa ttcttcttcc tcaataaatt aacaattcat tctttaccaa
     1801 atgctacttg aataaaattt cattatataa tgtattatca tttggtagtt atgaaaaaag
     1861 ttatactctt aattctaatt ctttctcttc tacattgtaa aaaagaagta gaaaaaccaa
     1921 aaaatgaaat acaattcctc tttacagatt ctttgactgg aactaacctt tatgagaatc
     1981 ctgaacctga ctcaaatatt attagcagta ttccacatgc taccaaagta gaaatcctac
     2041 cagaaaatcc tgcttacaca attaccaagg aaaatacaat caaatggcgt aaaatcaaat
     2101 ttgatacttt tgttggatgg gtaatggata atcttttgag cgcgaaagta gaaaaacatt
     2161 actttagtgt attaactaac gctggcttac ctctcagaga aaaaccagat gtgaaatcaa
     2221 ataaaattgc aacaattcca tttggttata taggcgaatt ttacaataaa acaaaggaaa
     2281 atgtcatttt agataagcag ccggggttct ggatcgagac aacttacaat ggaaaaaaag
     2341 gctggatttt ttccggatcg gcggcaattt ctaaaaactt acaatcactc gaagaacgca
     2401 atggggaatt tggtgaagaa ggctggttct atagattcta cagcaattta gaggagtcga
     2461 aattagaaga agtcgaattt gatctaaaaa atattgaaaa tacagccaag ataacccaat
     2521 tcgaaaacca cccttataca atctaccaaa tcaaacgtca ggttgcagaa gatgattgct
     2581 caggaaaaga aagtcagatc attttcaaaa ataatattac tggaaaaagt tttggtaata
     2641 gggaaatttt ttccgaaaca gtggtagaac aaaattaccc actagttcga tcggtttaca
     2701 caacatccat tccacaaaaa tgtggatgct taatccaaga ttcaactttg tatttcttat
     2761 tagacgatag agtattagct acaacgtttc acaataaaga caccaaagcc tattgtgaat
     2821 atggaactgt ttccaacata gaactagaaa gggagaatcg ctatgatctt gcaaacaata
     2881 ctctctatat gtatttaaag ttacctgatt gtcagccaga tgaaaaaaca attctatatg
     2941 gcggaaaaat aacgaatatc aagaaattta aaacagatat gttcgtttct ttaaaactat
     3001 ctaacactga agtaatgatt gaaaaattct atcaaaaggg aataccagaa cagtttaaaa
     3061 cagcatggga gtcatctgta aaggaaccaa atcttgtcaa agaatagaat cggaatcgat
     3121 atcggcggtg tcattatcgc agaggataca gacaagcctg atttattttt tagttctcat
     3181 tttctaaaag caaaaccagt ttcgaactca tttgaaacta tcaaagaatt aatcgataca
     3241 ttttcgagtg aaaatatttt tatcatttcc aaatgtggac cggaaatcca aaaaaaatct
     3301 ttattatggc tagaggataa tttctttttt caaaaaactg gatttaaacg agaaaatata
     3361 aacttttgcc tacatcgaaa tgaaaaagca atcattgcgg aaaatctgaa tttaacacat
     3421 tttattgatg acagatatac tgtattaaag cattttctgc cactttctca aattaaacaa
     3481 ctatttctat tcaaccctaa cgttgaggaa atgaggttaa tgggaagtat cccctcaaat
     3541 aaaataacaa tgatatcaag ttgggtagaa ataaagattg aatagaaatt taaacaacaa
     3601 tatcataact aatttttact ttcaatcccc aatttttctt ccacattttt cattctttta
     3661 aaaatttgat ttaaaatcgg taggtattta atattagctc taaacttttg aaaatctacg
     3721 atagacgcaa atacatccca gccagcatag agacctggct tcgtcacatc tgcacgcact
     3781 ccagtgcctg ctccaaagac agcgcctgaa caaatatgaa catgatccac agcactcact
     3841 ccgccgccca caattacact atcctctaaa acgacactac cggctaatgc tgatttaccc
     3901 gccagaatgc agttttttcc aattttgcaa ttatgtgcta cgtgcacttg gttatcaatt
     3961 tttgttccat caccgattgt agtatcacca atgccacctc tatcaatcgt gcaagatgat
     4021 ccaactcgaa cgtcatctcc aattacaacg gttcctactt gaggaacatg atgaaaaatt
     4081 ccttttttat caggaacaaa tcgaaaccct tctccaccaa ttgcactatt actaaaacac
     4141 aaaaatcttt ttccaatctt tacaccatga taaattacag tatttgctcc aattacagaa
     4201 tcatccccga tttctgctcc ttccaaaatt ctagctccgt ctgctaaaat acaatttttc
     4261 cctattttaa cgtttttttc aatggtaaca aaatttccaa tttcagtatt ttctccaata
     4321 gttgcagttg aatcgatact gacatattgc cctctttttc cggaagattt tttgggagga
     4381 aagaataaat tcaaaatcgg tgtaagcgca aaatctggat tatccacaac tactacaggt
     4441 attttaaaat gaacagataa ttcctttgtt gttacaatag cgccaagctt taatggcaag
     4501 acctctttca aataagactt tgatcctaaa aacaaaattg catcttcgcc ttggtcggga
     4561 actgcaactg gctttgctat tagaacggat gatggattat cgcaatttat aatttcagaa
     4621 ttcggtaaaa ctctttgaat atcacttaaa ctgaacattt tatacacctc attaaagcaa
     4681 aatttaatcc tatagctgat taaacaaata gaaaatttat ttctaagatt caaatatagg
     4741 attattcacc cctattatca cttgcttcta taacaaatga tttcaaattg taaatatgag
     4801 ttctagttat tttaagatta ttggtaaaaa tccgctttct ggaagtatca caccacaagg
     4861 aaataaaaat gaagcattgc ctgtgttagg tgctgtatgt atgattcagg gaaaagttcg
     4921 aattgaaaat gtcccattga tttcagatgt tctaatgtta atcgatgtat tacgatatct
     4981 tggatttaaa atagaaaatc ccgagccgac tgtttttatt tttgaaagac caagtgagtt
     5041 aaaatctgat ttgccgcaag aattatgcag taaaattaga ggagctgtta cattagctgg
     5101 tcctatttta gcaagcactg gtcgagtttt tttgccaaga cccggtggag ataaaatagg
     5161 aagaagaagg ttagatacac atctactcgc attagaatca cttggagcag atatagaagt
     5221 atttcctgat ggatatgaga ttaaagcaaa taaattaatc ggggaagata tattgttaga
     5281 tgaagcatct gtgaccggaa ccgaaaatgc agttatggct gcctctctag cagaaggaac
     5341 aacgattatc cgtcatgctg catctgaacc tcatgtgcag ggtttatgta gactgttaaa
     5401 tgcagctggt gcaaaaatat ctggaatcgg atcaaacata ctaactattg aaggtgtaga
     5461 atctttaaaa agtcctgaag gaaaagaaat tgttcataga atcggttctg attatttaga
     5521 ggttgggagt ttcatctcgt tagccgctgt cacaggagga gaacttttta taaatgatgt
     5581 aaatccagac gatatgcgaa tgataagact cgtttattca aaacttggaa ttgagattcg
     5641 atttaccgat ggtggcgttt tagttccagc tggacaaaaa ttggaaatca tccctgacta
     5701 tcacggagca actccaaaaa tcgatgactc tccctggcct ggatttcctg cagatatgac
     5761 gtcagtagca cttgtaaccg caacccaatg caaaggaact gtaatgattc atgaaaaaat
     5821 gtttgaatcc agactctttt ttgttgataa tattataagt atgggagctc aaatcatttt
     5881 atgtgatcct catagggcag tagttatagg caaatcacag ttatacggtg gaaaggtagc
     5941 gtcacccgac atacgagcag gaatggctat gattattgcc gctctctgtg cagaggggac
     6001 aagttatatt cataatatca tccaagttga cagaggattt caagatattg atactagact
     6061 tcgttctatt ggcgcacaaa ttgaaaggat tactgagtaa ttatgaatct acaggaaaaa
     6121 aaatgaaatt aaatcaattt tcttttaaaa ttctattttc agttctaatc caaattctat
     6181 ggatgacttc tgctttaatt gcggatgcaa gttttgattt aattcaagga gtcaaaaata
     6241 gaaaattgaa tcaagtttca aatgcattaa atatgaatgc agatccaaat attaaagtaa
     6301 acggcaatcg ccccatactt ttgatagcag ctgagaatgg agactttgaa attattcaaa
     6361 tgctattagc aaagggagcg caggtcaact cctttgaaac aacaaaagaa aatccaggtt
     6421 ggactgcgct catgcattca acagaaaacg gtcgcatgga tataatccga ttacttttgg
     6481 caaaaggagc caataaaaaa caaaaaaatg gaaagggata tgatgcaatc tatttagcca
     6541 aactttgggg caactctgaa gttgccgctt ttttagaagg tagggatata aacgaaaaaa
     6601 agctccaacc ttgcggaaaa atactaacag aaaaaaatct aaatcaaaaa aatatcagtc
     6661 ttgtggatct gaagaataat aaattttata aaaagagttt tctatctcgc tctacttaag
     6721 ttaggtgaga caaacaaaat aaaaacaaag agtggactat gaaatatatt tttgtatctt
     6781 taattggttt tttcctccta ttagaatcct gtaatacagt tacaacaggt gtatcagaag
     6841 cagtcgtaag acctcctagt ctaccatcta gcgtttcctt tgctggagaa aaagttcccc
     6901 tagatgatca agatgtttta gagagattag atagagagct agtcgtaaat caaaattatc
     6961 actcctctac aattctaata tataaaaacg ttaaacgcta caaagaacct atagaaagga
     7021 ttctaaaaga gaatggaatt cctgaagatt ttttctattt agcagttgca gaaagcgcat
     7081 taaatccaaa tgcaacatcc tctgcaaatg ccgttggtat ttggcaattc atccaagcaa
     7141 ccggggaagg ctatggtcta gaagttaaca attttgttga tgaaagaaga catttcgtaa
     7201 aatccacacg tgctgcctgt aagtatctac ttgatgcata caaagaattt ggaaattgga
     7261 cgttagtagc agcagcctac aatagaggac ttaaaggaat gaaagatgct gttacttctc
     7321 agaaaacaaa taattattac aaactttacc taaatcaaga aacagctcgt tatgttttta
     7381 gaatcatagc gcttaaactt attttaagca atccacagga ttttaatttt gatctaacag
     7441 aaagtatgca ataccctgct tataaactaa aatccattaa ggtaacggaa actattgcaa
     7501 gtcttcccga atttgcaatt tcaaatggga ctacatacaa agaactaatc cttcacaatc
     7561 catggattcg aacaggtaaa tataactttg atgttgctac tggaaaatca tatgattttt
     7621 taataccgga taactaggtg caattagaac caacggctaa tccatactta ttttggatat
     7681 aacaatggat tgtattgatt cctccagcat caaaacctgt ttcaaagaat atgacttcta
     7741 aaatttctgc atctagatag tttacaacgt ttaaacctgt cccgagcaag aaaacatttg
     7801 cagaagaaaa tgcggttgga gtagcaatcg ttccagttcc atctgccaca ccattataat
     7861 aataggttgt gacgccagac gcatccgttg aaaaaaggat catgttataa cctgttgttg
     7921 gaattgccgt tgtagccgcg ccagcacaat tagtaggagc tggtgataag ctagaacaaa
     7981 tactttgatt tgtagtattt gctttccaat ttcgtgcatt tggaacattt ccaagaaatc
     8041 caatactaaa ataaacctca tcggtttgag acgaatactt tcgcaatacc attgctccaa
     8101 cacccgcctt gcttcctata tatccagtgg cggatctaga attaaaaaac tgtttctgag
     8161 ttgaggttaa acgaacagca gctttaccat ttaacgcatt ggctactata gtaggacttg
     8221 atgttcctgt tgaacttgga tagagatgat ttccattccc actataatca ttccatactt
     8281 ttacttccag tccgggaata tttcctatgg agtccgctga ataccaaaag cgtaagttgc
     8341 tagctgccaa tggactaaga gtaacagtat attggtcagt ttttgtggct gctaccactg
     8401 agtcaacaca ggctgctgcc cttaaagtct gtgacttggc acctaatatt ggagcaggtg
     8461 cggtgtatat ggttccgttc gtgcaggagg gttcagaccc atctagcgta tatcgaattg
     8521 ttgctgtgga attagaagta attgttacaa acggatcgta tttataggtt ccccccggcg
     8581 gagaaaaagt aagaccatat ctagaatttc gaatagctct ctctaaaacg atagacgata
     8641 taggattgca acttgaatct agttgattgc atgtgcttgc attcttacaa ttttcaaact
     8701 gtaatataat tagaagtaga aaactagata ttaaaaaaat tcgcttcata gattactcct
     8761 tgtttactgc aaaatgtaat tttatatatc gctaaaactt gcaatttcaa gtgcaattaa
     8821 agtttcactt aaattgattg gcggatcaaa ataaagccag ataaaagtat aatgtctacc
     8881 tttgtgggtt tgattttcga tggaggttgt tttgccatga aattcgtatt tctcagcctg
     8941 acctcttttt ttcatataac cttttatatc ttgtgttgtt ttagaaacat gcattccttt
     9001 atgaacaata caaatctccc tttcacttgc ataccccaaa ataccgagca tgtgttttcc
     9061 taaatactca aatcctacaa aataatcctt gtagcgattt gctttaaata aaaattttcc
     9121 cttatctatc atatgttatg cctcttcatt aagatagcgt cttttccatc ggggtagtat
     9181 tttttacgga ctgcaaaaat tataaagtct ttttttttat agagttcgat tgcgggtgaa
     9241 ttttcactct taacttctaa aataatttct cgatttctcg aaatcgtaaa tacttcttct
     9301 aatagttttt gtgctactcc ttgtcttcga gattcaatca aaactccaat tcgtaaaatt
     9361 tcaacctcaa aaggattttc tagatagatt atataacctg taggcttcaa atcattatca
     9421 aaatagagaa tggaagaatt caataaatgg tgggattcta tttgggaaat gttccaagct
     9481 tcttcttcaa aacatttttt ttctagattc tcaaggattg gaaattcatc tagagaagtt
     9541 tttttaatca aaaattacac ctttcctacc ttttttgtat aaaccacgta attggaaata
     9601 aaataattag attgtatttt agtgatactt ttacctaact agatacaaaa ctgtttgcca
     9661 aaagaattta ccaaccacta ggaaatgctt taaaatcatt tcttatgaag ttataatgat
     9721 tcttgcagat agtttaaaat aattctattt caaatcgtgt gagaatctgg aaatctacct
     9781 gaacctctcg gagaaattag aaaagaatta aaacaagaaa ccaatcgata taaaagaaag
     9841 aggtatctat gaaatatttt ttattcacat tgatcttagt tttattttcc tgtgataaac
     9901 ttaaagaaat tggacaggaa aatttcaaaa agaacgttac tcccaagacc cctagcgaag
     9961 ccgaacttga gaaatggaaa gaaaaactag ctttagatga ggcagagatt aaagagctag
    10021 acgaaaaaat tcgcaaaatg gtaaagaaga caaaggaagc cggtgttctt tcttggagaa
    10081 tcgcacaagg atatatgaga gttggtaatt atgaactcag tagtcgttat tatgcacagg
    10141 cgattcaaca aaacatggaa aataaagtag acgttgttgg ttctgaagtt catttattcg
    10201 aagcggctat tccttttttt gaaaaggctt tgctttatag agaactaacg gatgacttgc
    10261 tttttgaagc aggtcttgcc tatgcgaatg catctagaga cagaggatgg gataaagaaa
    10321 gaaggaacat agctgtcaca atctttaaag gactcattcg aagagatcca gcagatttac
    10381 gttatccgta tgagcttgcc ttgatttatt ttgattcatc tatttccgat ggaggaattg
    10441 atggagtcga tccagcaggt tataacgata cagaaaaagc gatgcgaatt atgaatgcaa
    10501 tcattcaaaa agagccaaac aatattcctg ctaggtttgc gagagccaac tttttataca
    10561 gaagtggttc gactgagcaa gcaacggatg aatatctaac tattaaatcg aaactagaag
    10621 cgatgaagga agccggagta attaaagaga gtctagataa aaatacatct taccaaaacg
    10681 tagtgcgtaa tttaaaaaaa ataaactctg gtaaataaaa tggaagagtt gattcctttc
    10741 tatttctctt ttcaaagcta ttcttctatt gcacatagat ataatctttt aacagaagtt
    10801 tccagcttat ttgaactaga aaggaaaatt tcaccgatac ttaaatcaga agaaatatct
    10861 aagattaaat tagcctcagc tcaactaaaa aaagagaatg aaaaaagagg cattcaaaca
    10921 atcgattact ttcacccttc ctatccaacc ctgctgaagc aaattgacaa accgcctatc
    10981 gttttctttt gtttaggaaa tacaaatcta ttacaacaag accttgtagc tgtc
//
LOCUS       DAOQOS010000150        11030 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_3065_length_11030_cov_6.688929, whole genome shotgun sequence.
ACCESSION   DAOQOS010000150 DAOQOS010000000
VERSION     DAOQOS010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 11030)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11030)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11030
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3065_length_11030_cov_6.688929"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(153..1085)
                     /locus_tag="PLF66_22440"
     CDS             complement(153..1085)
                     /locus_tag="PLF66_22440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66864.1"
                     /translation="MQKPKTKRLFRKFILILCIAGILQNCLMVPREVLTRDLKNQKNP
                     FPENTIFYVTFVQNPVLEDTEYIVDSGEKINMIEEEIRNCNCIKKFKVLPLTSVNGKE
                     IKTIPYGSENSEYPNQIFLEFYTNQHSYQSLFNLFYFYDRDKKNKNSIFKSMSSNTKP
                     LSSFQENINSVLKIQSLQVAQSQQEAKLFEKILKEAIKSNNSKSSKKGGGGGAALLIL
                     AIVTFIFTVTVHYAQTDVHLNTTHFQNNKFIEKNNFVYTLDTFSHLFLIFKIPWTLPQ
                     THYNIAYKDLSSKFLNEFKKENLSLTSNDRVDID"
     gene            complement(1200..4430)
                     /locus_tag="PLF66_22445"
     CDS             complement(1200..4430)
                     /locus_tag="PLF66_22445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020775557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HMZ66865.1"
                     /translation="MSKQFISPQKFLKFLVGFIFSIPVFIICFSFSYYIFNENVKQFY
                     DLTDYKLKIAAYTVKHILPNDYHDRIEDSKSISKEEFSNINESLNKLIHEIQIEYVYS
                     MIKVNGKFYFTSANSKAKDVIRKIDPAFFEEYTEEKYGEISKIDMNLSRPQLIRSVDK
                     WGEFRSILIPMHTKNGKEYVLGADISTHKLQKTIHFFLIISVLLGIILFIPAFIITFD
                     LVNQGKVYQYIFLKKKRKGFYISFLFSVVLTMFFGFYNYSLKRDQIFSYIDSILFTGA
                     SGLKNILPRNYHEGLDLITNNSYEHIKNELNEFASRIGFDSLNSIVERNGKFYYTASN
                     VLKSDTDNSTETSFLQEVKSETSYFKEALNKNKILYLNELNTSWGNFHRIIIPIKSIA
                     KKEYILSADIQLQQIQNRIKQSTLELIIRDIAFFLFIFLSTTKIFISLSNLEIQKNYR
                     EWFQFISLRIKLGFFISSLIFFCLIAVSYIYFYNSKIILQKKTLEVCRNFATNISNIA
                     REDLIEDTTYNATNSAVSEILKSDIEGLLDVYIINVYGKYVVDFNKVKINEYASETLI
                     SYIQSIHSIDLTEEYSTQTNRNILKISYPIFIEYNEQPIKIGVAIFEYDRDVIYQPVY
                     DMQSAVILTGLFALIITLIASQFLSSYITRPLLVLEKGAQIIAAGNLDHIIPVKTNDE
                     VGLLSKRFNEMSLNLKKSYDELEEKVIERTADLVKSQATLTTVLSNAPLMLFSADTNA
                     IITLSEGQSLNLIGIGSGQAVGISMYEMFEDNKEVLEAIDSALNGEGKIMKLKIEQFT
                     FEVNYSPLYNLENKIVGLISVYFDVTEKLKAQEIINSEKEKSDKLLLNILPEKIANEL
                     KETGHVKPVLYDSVTVIFTDFKGFTKIASNMSPEELLEKLDMIFLQFDQICERRKIEK
                     LKTIGDAYMCAGGLPETNLTHPIDACLAAIEMQNFMNETKAIIEQISGEEFWDMRLGI
                     HTGTVVAGVIGKTKFAYDVWGDAVNTASRMESNGSIGKINISHATYNKVKDFFECEYR
                     GKIEAKNKGMIDMYFLLRIKPELSRDKEGIVPNDEFIDLYNKTLYG"
     gene            complement(4423..5979)
                     /locus_tag="PLF66_22450"
     CDS             complement(4423..5979)
                     /locus_tag="PLF66_22450"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HMZ66866.1"
                     /translation="MINTGKRRFISIKVKISILTSAVAVFCLFTISSVFLYNSKIIMQ
                     NRTIEICRNFAENISNVAREDLVLDATYESTNSVVGEIIKSKIEGLQSIYIVNVYGKY
                     VVNFYKANLPDVANNQELAYYQKIQSLDLQEVYLKESKQNILKITYPIFIKYNEQNLK
                     IGAAIFEYDRDQIYAPVYHIQNRIIFLSIGITTLTLIITFFVSSYITKPIIQFSKGVQ
                     MLASGELAHKIEINSRDEIELLSQKFNDMSFNLRQSYEQLEEKVLERTKELNSTLNLI
                     RKDLAIAEKIQKTSLALNLAKYTDLEIVIKYVSMTEVGGDFYAVNKLSDSTSRFFLAD
                     ATGHGVQAALIMMAIQGIYDSIKNFDLPPNEVLEIFNREFNRRYGILNTFLTCILLDV
                     DTSNHNIKYASAGHPPGILIKENNEFLLLNKTGPLLGAKPNCKYSQVEYPFLQNERIY
                     LYTDGIFEQFYQEDEFGEERLHKILVDNQKNKLDVCIDNVLSELNTFLHYLPKQDDLT
                     IVAIAHKGNV"
     gene            complement(5986..7554)
                     /locus_tag="PLF66_22455"
     CDS             complement(5986..7554)
                     /locus_tag="PLF66_22455"
                     /inference="COORDINATES: protein motif:HMM:NF016292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HMZ66867.1"
                     /translation="MKYFRELFLILLICINQIIAEEESIEILLSSNNSIYLTTLQSIQ
                     SSSKRKLNLHFTSGMNEVELKDFFSSIEKRNIPFLITLGPQASLLAKDNLVNVPILYS
                     LINSPRSLGFNYKSNACGIHIDVPIQEFFRALKDIKPSISHVAAIYSNNTGEQLINEA
                     DYNDSLYGILFQKIKVENKTDFSEKLIPLKGKIEAFYIVPDAIYTQENFEIISKFAKE
                     NKIILMTQIPFIVNVGTTFSITPYYARVGALIGDLATEIISGKTKCKNGYANAVKEFS
                     LSLNKTYAEESEISLPYNILKRADNSRLLIEGINFFEKGNLEVSSLVMEKILKDDPTN
                     YTAFYYKNTINQRVNGDKIQDYFIKARDARNSKNYKRERDYLNQILTLQPENENAKQG
                     IQESLLLESESERAEGERLENSNQIFVAIQKYLNSVKLLDSNTKAKADLTRLRQKELK
                     NVPIYIQKGKSLYEARKYSDSEEMFQNILLVEPENKNAKEYLRLSKEKRLAMIKYENC
                     IKEADKKCYLLWEK"
     gene            7702..10614
                     /locus_tag="PLF66_22460"
     CDS             7702..10614
                     /locus_tag="PLF66_22460"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HMZ66868.1"
                     /translation="MNLFLRFILFVLILSSSLLSKDIQKKVMIGQFIPHKSEVDLKIE
                     EKLQASLISSFQSRGFDVDQVSEGKAVNMDKAQKIKALYYIEGYYQVKKDKKILTIYA
                     LLYDPKTGNLVDVIRNSNDIADSLGDSFKDIDDKYKEDEDKVIQTLSTSVSFSVLTNP
                     SKTEIRENIYDQLLSRPIGKEINVSLAKEDKTEKSKEILKQFGEEEVITASRSSQKIS
                     DAPSKVIVFSRDTIRERGYRTLTELLQDVPGFDFNSFNDSGEYPTDLIIRGISDVGQT
                     QILLMENGIIQNDIGNGWLRHVQFDTVLIDVERIEIILGPGSSLYGANAYAGLINIIT
                     RKGKSLFSKKNANVMGDARLQAGKYNTYMPEGLFAFKFPNDMIFQMAGRYYNTNGDRG
                     MNRPDPGNYFHNNFEPDKVKTTEYGVVNNDLTPFGTRKPLKDGFNNSARDFFLRGALS
                     KEGFTLGFNLWDIKEGLSSYVPGYEYFSNTSGIPFMKHHRGYYINASYETSITQKLNS
                     MSKIYYRNTSIMPDTGFEYTYQYQGLDFPIDKTGQVRGATFDKAKTYYGPSYLTGAQQ
                     QFNYTATETNNLVFGFQLDKFIRQSISEGNGGVSLGRRQNVNSSAVLSSYDSGISSTA
                     TVFYSTTIASYIQDEQKFFNDRLSFTLGIRHDQDTNYGSIWTKRAAIIGKPIQWYNIK
                     LLYGEAFKAPTVFQLYDSFRGNIGLKPQQIKTYEIENSFFINKMTTIKAGYFMSLLDG
                     LIAEGNNPDPFTANTRPSIFQNFKPTHIYGYSLEGDIAITNEIKIFGNYTITRDRDVK
                     TQFTVLTNSLGSITAINPIYDGREIDNIAEKKANFGVNFLFFKKLNVNLRMNWVGRRK
                     APLTNRYFQPFDPTFISKKYPYETEGKPDGYMSSYTLFNATVTYRDLLNIEGLEIQLV
                     CRNILNKSYSGMGRQSGNATRPIDSVQPTISNPDGFVSPYHPQPGREVFLQIAYMF"
BASE COUNT         3565 a         1724 c         1767 g         3974 t
ORIGIN      
        1 agagataata agagtcagcc caaagaattt ttattcacaa aaataaagga acaaatattt
       61 ctttttttga tgactccgct agatctaatt acttctcctt ttcgtttttt tattcataga
      121 attctataga atcctcattt tacttttcca atttaatcta tatcgactct atcgttactc
      181 gtaagactta ggttttcttt tttgaattca tttagaaatt tagaagataa gtctttataa
      241 gcaatgttat aatgtgtctg tggtaaagtc caagggattt tgaatattaa aaataaatgc
      301 gaaaaagtat ctaaagtata tacgaaatta tttttttcga tgaatttatt attttgaaag
      361 tgagttgtat ttaaatgaac atccgtttgc gcataatgga cagtaaccgt aaatatgaaa
      421 gttacaattg ctaaaattaa taacgcagct cctccgcccc ctcctttttt ggaagattta
      481 ctattgttgc ttttgatagc ttctttcaag attttctcaa ataactttgc ttcttgttgg
      541 ctctgtgcta cttgtaagct ttgtattttt aaaacagaat tgatgttctc ttgaaaagac
      601 gataatggtt tcgtattaga tgacatactt ttaaaaattg aattcttatt ttttttatca
      661 cgatcataga agtagaaaag attaaataaa ctttgataac tatgttgatt tgtataaaat
      721 tctaaaaata tttgattggg atactctgaa ttttctgaac cgtaaggaat cgttttgatt
      781 tctttcccat ttacagaggt taaaggtaga accttaaatt tttttataca attgcaattt
      841 cgaatttctt cttcaatcat atttattttt tcacctgaat ctactatata ttcagtatct
      901 tctaaaactg gattttgcac gaatgttaca tagaagatcg tattctcagg aaatggattc
      961 ttttgattct ttaaatctct cgttaaaact tctcttggaa ccatcaagca gttttgtaaa
     1021 attcctgcta tacaaagtat taatataaat tttcgaaata gtctttttgt ttttggtttt
     1081 tgcattctat ttatttccta acttttagga aagagaataa ataaaaataa gcagtttgtc
     1141 tataagtgga taacctaatt agtaattcta cctatgagaa tttatttaag tagtgggaat
     1201 cacccataca atgtcttgtt ataaagatct ataaactcat cattcggaac aatcccttct
     1261 ttatcacgag agagttccgg ttttattcta agcaggaaat acatatctat cattcctttg
     1321 ttcttagcct caatttttcc tctatactcg cattcaaaaa aatcttttac tttgttatag
     1381 gttgcgtgag aaatgttgat tttaccaatt gagccgttag actccatacg agatgctgta
     1441 ttgactgcat cgccccatac gtcataggca aattttgttt taccaattac accggcgact
     1501 acggttcctg tgtgaatacc caacctcata tcccagaatt cttctcctga gatttgctca
     1561 atgattgcct ttgtttcatt cataaagttc tgcatttcaa tcgctgcaag acaggcatca
     1621 ataggatgcg ttagatttgt ctcgggtagt cctcctgcac acatataagc atcaccgatt
     1681 gtttttagtt tttcaatctt tcttctttcg cagatttgat caaattgtag aaagatcata
     1741 tctaattttt caagcaattc ttccggtgac atattgctag caattttagt aaatcctttg
     1801 aaatcagtga atattacagt gacggaatca tagagcactg gtttcacatg tccggtttct
     1861 tttaattcat ttgcaatttt ttcagggagt atattcagta aaagtttatc tgacttttcc
     1921 ttttctgaat taatgatttc ttgtgctttt agtttttctg ttacatcgaa gtaaacagaa
     1981 attagcccca caattttatt ttctaggtta tagagaggag agtaattaac ctcaaatgtg
     2041 aattgctcaa tttttagttt cattattttt ccttctccgt ttagtgcgga atcgattgct
     2101 tctaatactt ctttgttatc ctcaaacatt tcatacatag aaataccgac tgcctgacca
     2161 cttccaattc cgatcaaatt tagactttgt ccttctgaaa gagtaataat agcatttgta
     2221 tctgcagaaa aaagcattag gggtgcgttc gataacacgg tagtaagtgt cgcttgtgac
     2281 tttactagat ctgcagtcct ttcaataact ttttcctcta gttcatcata agattttttt
     2341 aaatttagac tcatctcgtt aaatctctta gataagagtc cgacttcgtc atttgttttt
     2401 actggaatta tatgatctaa atttcctgcc gctataatct gagcaccttt ttctaagact
     2461 aataacggtc tagtaatata agaagataga aattggcttg cgataagagt gataatcaaa
     2521 gcaaaaagtc cagtcagtat aacagcactc tgcatgtcat atacaggttg ataaataacg
     2581 tccctatcat attcaaaaat tgctacacct atcttaattg gttgttcatt gtattcaatg
     2641 aagataggat aggaaatctt taagatatta cgatttgttt gcgttgaata ctcttccgtt
     2701 agatcgattg aatgaataga ttgaatataa gatataagag tttcagaagc atattcattt
     2761 attttcactt tattaaagtc aactacgtat ttcccataaa catttataat ataaacatct
     2821 aaaagacctt ctatatcgga ttttaaaatt tccgaaacag ctgaatttgt tgcgttatac
     2881 gttgtatcct caattaaatc ctctctagca atattcgaaa tattggttgc gaagtttctg
     2941 catacttcta gtgttttctt ttgtaaaatg attttgctat tatagaaata gatgtaagaa
     3001 accgctatta aacaaaaaaa gataagacta gaaataaaaa atcccaattt tatacggagg
     3061 ctaataaact gaaaccactc tctataattc ttttgaattt caagattaga gaggctaata
     3121 aatattttgg ttgttgataa aaagataaat aaaaaaaatg caatgtcgcg tatgattaat
     3181 tcgagagttg actgcttgat tcgattttga atttgttgca attgaatatc tgctgaaaga
     3241 atatactctt tttttgcgat actttttatt ggtattataa ttctatgaaa atttccccag
     3301 cttgtattta attcatttag atataatatt ttatttttgt tgagcgcttc tttaaaataa
     3361 ctagtttctg attttacttc ttgaagaaag gatgtttcag tgctattgtc agtatctgat
     3421 tttaaaacat tactagcggt ataataaaat ttgccgtttc tttccactat ggaattaaga
     3481 gaatcaaatc caattcggga agcgaattca tttaattcat tttttatatg ttcgtaagaa
     3541 ttgtttgtta ttaaatccaa tccttcatga tagtttctag gtaaaatatt ttttaaacca
     3601 gaagcacctg taaacagaat agaatctatg taactgaaaa tttgatctct ctttaaagag
     3661 tagttataaa atccaaaaaa catagttaga actactgaaa ataaaaaaga gatatagaat
     3721 ccctttcttt tcttttttaa aaaaatatac tggtatactt tcccttggtt tactagatcg
     3781 aatgttataa tgaatgcggg gataaataga attatcccta aaagcacaga tattattaag
     3841 aagaaatgga tggttttttg taatttatga gtgctaatgt ctgcacctaa aacatattct
     3901 tttccattct ttgtatgcat tggaatgaga atcgatctga attctcccca tttatcgact
     3961 gagcgaatga gctgaggtct gcttaaattc atatctattt ttgaaatttc accatatttc
     4021 tcttcagtgt attcttcaaa aaatgctgga tctatttttc gaattacgtc ttttgcctta
     4081 ctatttgcag atgtgaaata aaacttaccg tttactttta tcatgctata aacatattca
     4141 atttgtattt catgaatcag tttatttaaa ctttcattga tattagaaaa ttcttctttt
     4201 gagatagatt ttgaatcctc gattcgatca tggtaatcgt taggtaatat gtgcttaacg
     4261 gtataagcag cgatttttag tttataatct gttagatcat aaaattgttt tacattttca
     4321 ttgaatatat aataagaaaa actaaaacaa attataaaaa caggaatgga aaaaataaat
     4381 ccaacaagaa attttagaaa tttttgtggt gaaataaatt gtttagacat ttcctttatg
     4441 cgcgatagct actattgtta aatcatcttg cttaggtaga tagtgcagga atgtatttaa
     4501 ctcagataaa acattatcta tgcatacatc taatttattt ttctgattgt cgactagaat
     4561 tttgtgaagt ctttcttctc caaattcatc ttcttgataa aattgttcaa agattccatc
     4621 tgtataaaga tagattctct cattttgtag aaatggatat tccacttggg aatatttaca
     4681 atttggtttt gccccaagga gaggaccagt tttatttaaa agaaggaatt cattgttttc
     4741 tttgattaaa attccagggg gatgtccagc tgaagcatat ttgatattat gatttgatgt
     4801 atctacatcc aataaaatac aggttaaaaa tgtatttaat ataccataac gtctattaaa
     4861 ctctcgattg aatatttcta aaacttcatt cggaggaaga tcaaagtttt ttatggaatc
     4921 atagattccc tgaattgcca tcataatgag tgctgcttgc actccatggc ctgttgcatc
     4981 tgctaagaaa aagcgacttg ttgaatcgct taatttatta acagcataga agtcacctcc
     5041 aacttctgtc atagatacat atttaataac gatttctaaa tcagtatact ttgctaaatt
     5101 taatgctaag ctagtttttt gaattttttc tgcgatcgca agatcctttc tgattaaatt
     5161 cagagtagag tttaattctt ttgttctttc taaaactttt tcttctaatt gttcatagga
     5221 ttgtcttaga ttaaaactca tgtcgttaaa tttctgggag agtaattcta tctcatctcg
     5281 gctgtttatt tcaattttat gtgctaactc accagaggct aacatttgca ctccctttga
     5341 aaattgaatg atcggttttg ttatataact agaaacaaaa aaagtaatga tgagggtaag
     5401 ggttgttatc ccaatactta aaaaaatgat tctattttga atatgataaa ccggagcata
     5461 aatttgatct ctatcatatt caaaaatggc agcgcctatt tttaaatttt gctcattgta
     5521 ttttataaag attggataag taatttttaa gatattttgt tttgattctt ttaaataaac
     5581 ttcctgtaga tctaaggatt gtattttttg gtaatatgct aattcttggt tgtttgccac
     5641 atccggtaga ttagctttat aaaaatttac aacgtatttt ccgtatacat tcacgatata
     5701 aatactttgt aatccttcta ttttagattt aatgatttct cctaccacag agttagtcga
     5761 ttcataagtg gcatctaata ctaagtcttc tcgtgcgaca ttggaaatat tctcagcaaa
     5821 atttctacat atttcaatgg ttctattttg catgataatt ttactattgt ataaaaaaac
     5881 agaagagatt gtaaatagac agaagacagc aacggcagaa gttaaaatcg aaattttaac
     5941 cttaatacta ataaatcgcc tttttcctgt attgatcatt ctaaattatt tttcccagag
     6001 cagataacat ttcttatccg cttctttgat acaattctca tattttatca ttgcgagcct
     6061 tttttccttg gaaagtctta gatattcctt tgcattttta ttttctggtt caactaaaag
     6121 aatattttga aacatctctt ctgaatcaga atattttctc gcttcatata aactttttcc
     6181 tttttgaata tagataggaa cgttttttaa ttctttttgt ctcaatcgag tcagatcagc
     6241 ttttgctttt gtgtttgaat ctaataattt aaccgagttt aaatatttct gtatagcaac
     6301 aaagatttga ttggaatttt ccaatctttc tccttcagct ctctcagatt cactttcgag
     6361 tagaagcgac tcttgaattc cttgtttcgc attttcgttt tcaggttgaa gggtaagaat
     6421 ttgatttaga taatctcttt ctcttttgta gtttttggaa tttctcgcat ctctggcttt
     6481 gataaaataa tcctgaattt tatctccatt taccctttga ttgattgtat ttttataata
     6541 aaaagcagta taatttgtag ggtcatcctt taaaattttt tccattacga ggctgctcac
     6601 ttctagattt cctttttcaa agaaattaat tccttcaatc agcaaacggc tattatccgc
     6661 tctttttaaa atattataag gaagagaaat ttcagattcc tctgcgtaag ttttatttaa
     6721 gctcaatgaa aactctttca cagcatttgc gtatccattt ttacattttg tttttcctga
     6781 aataatttca gttgcaagat ctccaattaa agctccgact cttgcataat aaggagttat
     6841 gctaaaagta gttcccacat ttacaataaa agggatttgt gtcattagta tgattttatt
     6901 ttctttcgca aactttgaaa taatctcaaa gttttcttgt gtatagattg catcaggaac
     6961 tatataaaat gcctcaattt tacctttgag tggaattaat ttttcagaaa agtctgtctt
     7021 gttttctact ttgattttct ggaagagtat tccgtagaga gagtcattgt aatctgcctc
     7081 attgatcagt tgttctccag tattattact ataaattgca gcgacatgcg aaatactagg
     7141 tttaatgtct tttagtgcac gaaagaattc ctgtatcgga acatcgatat gaatcccaca
     7201 tgcattagat ttataattaa aaccaagaga tctaggtgaa ttgatgagcg agtataaaat
     7261 cgggacattg actagattgt cttttgctaa taagctagct tggggcccta gtgtaattag
     7321 aaagggtata ttcctttttt ctatactcga aaagaaatcc ttcaattcta cttcattcat
     7381 tccactagta aaatgtagat ttaattttct tttggaggaa gattgaatgc tttgtaaagt
     7441 tgtaaggtat atactattat ttgaagaaag tagtatttca atagattctt cctcggctat
     7501 aatttgatta atacagataa gtagtattag gaaaagttct ctaaaatatt tcatagtaaa
     7561 tgccatttaa caggaaggat taacaagtat tatcccttag tcgaatctaa attgataata
     7621 acttaatttt caccttgact tataggtata gataaatagt ttccttgaaa aaggtaaagt
     7681 aaaatctttt taaatcctac tatgaattta tttcttcgtt ttattttatt tgttttgatt
     7741 ttatcctctt ctcttctttc aaaggatatt caaaaaaaag taatgatcgg acaatttatt
     7801 ccgcataaat cggaagttga tttaaaaatc gaagaaaaac tccaagcttc tctaatatca
     7861 tcctttcagt ctagaggttt tgatgtcgat caggtgagtg aaggcaaagc ggttaatatg
     7921 gataaggcgc agaaaatcaa ggctctttat tacatagagg gatactacca agtaaaaaag
     7981 gacaaaaaga tattaacaat ctatgcactc ctctacgatc caaagactgg gaatctagtt
     8041 gatgttatcc gcaattcaaa cgatatagct gattctttgg gcgatagttt caaggatatt
     8101 gatgataaat acaaagaaga tgaagataag gtaattcaaa ctctttcaac atcggtctct
     8161 ttttccgtcc taacgaatcc ttccaaaact gagattcgag aaaatatcta tgatcaactt
     8221 ctctcccgtc ctattggaaa ggaaatcaat gtatcgcttg caaaggagga taaaacagaa
     8281 aaatcgaaag aaatattgaa acaatttgga gaggaagagg tgatcacagc ctctagatcc
     8341 agccagaaaa tatcagacgc tccttccaaa gtcatcgttt tttcaagaga tacaatccga
     8401 gaaagaggat accgaacttt aactgaatta ttgcaagatg ttcctggatt tgattttaac
     8461 tcatttaatg attcaggtga atatccaacg gatttaatca ttcgtggaat ttcagatgtg
     8521 ggacagacac agattctact catggaaaat ggcatcattc aaaatgatat tgggaatggc
     8581 tggctcaggc atgtccagtt tgatacagtc ttaatcgatg tagagagaat tgagattatt
     8641 ctaggtccag gttcttctct ttatggagct aacgcttatg ctggtttgat taatatcatt
     8701 actcgaaaag gtaagtcatt gttttcaaag aaaaatgcaa atgttatggg cgatgcaaga
     8761 ttgcaagcag gtaaatataa tacctatatg ccagaaggtt tatttgcctt taaatttcca
     8821 aacgatatga tattccaaat ggctggaaga tattataata ccaatggaga taggggaatg
     8881 aatcgacctg atccaggaaa ttattttcac aataattttg agcctgataa agtaaagaca
     8941 acagagtatg gtgtggttaa caacgattta actccttttg gaactagaaa gcctttgaaa
     9001 gatggattta ataactcagc tagagatttt tttctaagag gtgctctttc gaaagaaggt
     9061 tttactcttg gattcaatct ctgggatata aaagagggct tgagttctta tgtgcctggt
     9121 tatgaatatt tttccaatac ttctggtatt ccatttatga agcatcatcg aggatattat
     9181 attaatgcat cctatgagac gagtataaca caaaaattaa attcgatgag taaaatctac
     9241 tatcgaaata catcgattat gccggataca ggctttgaat atacttatca ataccaagga
     9301 ttggattttc ctatcgacaa aactggtcaa gttcgaggag ctacttttga taaagcgaaa
     9361 acctactatg gacctagtta tctaacagga gcacagcaac aatttaatta cactgcaaca
     9421 gagacaaaca acttagtatt tggttttcaa ttggataagt tcattcgaca atccatcagt
     9481 gaagggaatg ggggtgtttc tttgggaaga aggcaaaacg tgaattcgag tgctgttctt
     9541 tctagttatg atagtgggat ttcatctacg gctactgtat tttattctac aacaattgcg
     9601 agttacatac aggatgaaca aaagtttttt aatgatcgtc tatcgtttac tcttgggata
     9661 cgacatgacc aagatacaaa ttatggaagc atttggacca agagagccgc gattataggt
     9721 aagcctatac aatggtataa tattaaatta ttgtatggag aggcttttaa agcgcctacc
     9781 gtattccaat tgtatgattc atttagggga aacattggtc tgaaaccaca gcaaattaag
     9841 acctatgaaa tcgagaattc tttctttata aacaaaatga caacgatcaa agcaggatat
     9901 tttatgagtt tactagatgg attgattgcc gagggaaata atcctgatcc atttaccgca
     9961 aatactagac cctctatatt tcaaaatttt aaacctaccc acatttatgg atacagttta
    10021 gaaggggata tcgcaatcac gaatgaaatc aagatttttg gaaattatac aattacaaga
    10081 gatagagatg taaaaactca atttacagtt ctgacaaatt cactggggag tataacagcg
    10141 attaacccta tttatgatgg aagagaaatt gataatattg cagaaaaaaa agctaacttc
    10201 ggagtaaatt ttttattttt caaaaagcta aatgtaaatc ttagaatgaa ttgggtcggt
    10261 aggcgaaagg ctccactgac caatcgctat tttcagcctt ttgatccaac ctttatctct
    10321 aaaaaatatc cttatgaaac agagggaaag ccggatgggt atatgtctag ttatacatta
    10381 ttcaatgcca ctgtaacgta tcgtgatcta ttgaatattg aaggtcttga gattcaacta
    10441 gtttgcagaa acattttgaa taaatcctat tctgggatgg gaagacaaag tggaaatgcg
    10501 acacgtccaa tagactcagt gcaaccgacc attagcaatc cggatggttt tgtgagtcct
    10561 tatcatcctc agcctggacg cgaagtattt ttacaaattg cgtatatgtt ttaacgtcag
    10621 tttgacttaa cagaaaaatt aaaagattac caccgaagct tcgctaacac cgaggacacc
    10681 gataaaagat acgataattc aaaattatac attgtgtctt catagttccg ttatcggtgt
    10741 tgtgagaatt acttcgagtt ctgcttttat taacgataca ctaaagatgc cctaaagata
    10801 ggactaaact tcccttaaag ataggtagcg agatatattt aggatatgtt tacccaatgt
    10861 ttacccctaa gtttaaggga agtttggtga tacgataggt aaaacattca ttctccacca
    10921 tgtcagtgta gatttttttc ttcaaagacc gcacatacat acgaattttt tatccatata
    10981 cagtctgttg cgaaatgtta aagaattaaa agtcagcctt taaaaataaa
//
LOCUS       DAOQOS010000151        10982 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_3092_length_10982_cov_5.885147, whole genome shotgun sequence.
ACCESSION   DAOQOS010000151 DAOQOS010000000
VERSION     DAOQOS010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 10982)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10982)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10982
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3092_length_10982_cov_5.885147"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..736
                     /locus_tag="PLF66_22465"
     CDS             <1..736
                     /locus_tag="PLF66_22465"
                     /inference="COORDINATES: protein motif:HMM:NF012876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HMZ66869.1"
                     /translation="YKFISSAKDDPKELETRFSDWEKISYFFTQKIYETGEKQFGKNY
                     LELIQKEKKRLKEFYPIAIEFIESLRELDDKSIRYHCFLLFSPSYNISSRGYIIDLNG
                     NRFLTYRYIQPNKNKMTIVGYSYESYRKYIHNLTINSLYVFLIGLFLFLIGIPFLLSR
                     TLSEPLKELLSGVKRVEEGELNTIVKIKYQDEIGYLAQSFNKMVDSIKDSKQKLEDYA
                     ENLESKVEERTRELKQTLEQVESLKE"
     gene            785..1999
                     /locus_tag="PLF66_22470"
     CDS             785..1999
                     /locus_tag="PLF66_22470"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HMZ66870.1"
                     /translation="MNEAIHTNIQVQFFTKQKKEFYFKKKKHQIGGDICIAQVIQLKN
                     KKYIVFLNADAMEKSIQGAGGILVLGSVFQSIIQRTKNYPLESENYPEFWIKKAFKEL
                     HKVFETFEGSMLVSMVLGLVEEKTGLVYYMNAEHPLVVLYRDGKASFLKERKLFNKLG
                     HLGMDSEIFVSTFQMLPGDQLIIGSDGKDDFIVSKTELNRKINDDESIFLEKVEKTNG
                     EIQKLYEVISSEFELIDDFSLLAINYPNDSNAFNHSEEVIQQKIANAKFALENPINKE
                     EGLLLLDSLYSDYPNQEEVNKIIIEYHTKHSNYQKAKEIYSKFIQHSESSTEILFLGS
                     QLEKRIGNYEESIKIAERVKLRDPNHLKNLIHLADLYLFTNNKERARKIILKIKKIDP
                     KHEKLKVLESKL"
     gene            complement(2358..3317)
                     /locus_tag="PLF66_22475"
     CDS             complement(2358..3317)
                     /locus_tag="PLF66_22475"
                     /inference="COORDINATES: protein motif:HMM:NF019224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1554 domain-containing protein"
                     /protein_id="HMZ66871.1"
                     /translation="MDTKIYLTVFFFLFPSCNNPEYDSPCTFDKSSFQKVYLLKQFTN
                     DTSSFCGIENKKVNSSSYTGANSSSISISYTGSPYTITALNSVGSILPQVVGTFTSYS
                     ISPSLPAGLNFNPSTGEITGTPSFLISSGNYTVTAISSLGTQTANLTLNIILPGKRIF
                     LSGNGILGNWATTSAADNFCNSDPAKPLGPGIYKAMIGFVSRKACNNGGGCVGFSAEA
                     IDWVLFAGIKYYRPDGTFIAQATNDRIFTFPLTNPISNISTNVWTGIDLGWNTTAAST
                     AECTGWTSAGFGNYGNSNATTNSILYVSNSVCGSSYRLYCVEQ"
     gene            complement(3584..5044)
                     /locus_tag="PLF66_22480"
     CDS             complement(3584..5044)
                     /locus_tag="PLF66_22480"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF017081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HMZ66872.1"
                     /translation="MQKTESNDSHTLKIIIKYISFSILYIGFAIIGMWLTSRPPSSLS
                     VLWLPAGIGLVSILVFGKKGLILVYLSSSIANGGYGFFSATEDRWAALYSGLLGAVVD
                     LIQTSICYFLYKTYRISFLTIKDFFLFFLRFCLLPPILTVWSLPLIWHYIGKVPMSDS
                     IPIYAVKVIQIVSADMLGIFLVTPLFFSLRRYHSLEILSLSFRIGISLLLPVLLALYV
                     HIPLISLIVPVILVYVLRYRFAGSTLSLTYTTGYLFLYLGISGSNLTNSDWFSFFNIS
                     FLILCIGFVPHYIAITLEQLRTVRKTLELKVKERTKELISANQKLEMMANTDLVTGLL
                     TRRAFLNRFKYESDRFLRTGRVFSIAILDLDHFKQINDSHGHSVGDEVLLAFSQIMIS
                     HLRITDIACRWGGEEFIILFPDTTIKEASISLNRILTFTETSEDLSSNVSIRLTVSIG
                     VTDSIVSNLDSIISNADKAMYAAKSSGRNKICLSHG"
     gene            complement(5745..5918)
                     /locus_tag="PLF66_22485"
     CDS             complement(5745..5918)
                     /locus_tag="PLF66_22485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66873.1"
                     /translation="MFIFQESFYLFSGKESFSTPSQKESRGKEKLISRISNNFRNRMI
                     ILQNQINSKHYSL"
     gene            5917..6072
                     /locus_tag="PLF66_22490"
     CDS             5917..6072
                     /locus_tag="PLF66_22490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66874.1"
                     /translation="MPIFPIQKQKRLGSKKIFSSSSQRYRIVIYFVSVINLFSITVHF
                     KELETSI"
     gene            6616..7503
                     /locus_tag="PLF66_22495"
     CDS             6616..7503
                     /locus_tag="PLF66_22495"
                     /inference="COORDINATES: protein motif:HMM:NF003784.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose bisphosphate aldolase"
                     /protein_id="HMZ66875.1"
                     /translation="MNKDMFQQMKSGKGFIAALDQSGGSTPKALALYGLNESSYSNEA
                     EMYDRIHEMRSRLIKSPAFNGDRIIGAILFEMTMERTIDGMGTAEYLWNKKKVVPFLK
                     VDSGLANEANDVQLMKPIPELGARIAAANKHGVFGTKMRSVINLANATGIEEVVKQQF
                     EVGKEIIAGGLVPIIEPEVNIKSPEKAEAEEILKKALLKYADGLKSDQNIMLKLSIPT
                     KVNLYKELIEHPRVVRFVALSGGYSREEANRLLALNHGIIASFSRALTEGLTAQQSES
                     EFNSALDSTIQGIYDASIT"
     gene            7750..8778
                     /locus_tag="PLF66_22500"
     CDS             7750..8778
                     /locus_tag="PLF66_22500"
                     /inference="COORDINATES: protein motif:HMM:NF019224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1554 domain-containing protein"
                     /protein_id="HMZ66876.1"
                     /translation="MLLYYKKKFLLLLLVLVSCQPVEYQNPCETTSKSFQKLYITQTI
                     TNSTQGFCGITSSKNSFGSNGADSGKNSFLISSPTLGYTLKNGGTDTFTVKLSAQPSA
                     SVTVPLFVSNPAQASVFPSSITFTTANWNSPQTVTVAGIDDLMYGVAGSFTVNLGPSS
                     SPDLLAQNITGSTVSISNRDFRKLIFVSNLTTGNTGGISGADAFCNSDSNKPINTGIY
                     KAFLTLPGVRVASVTANAGDGQIDWVLKPNQLYTRPNGLPIIITNSVSLFTAFPLINS
                     VYTAGDAIWTGFQGTSDWISIGGCVGWTSTGAFGTYAVGNAVNSTSLLFGGNLSGANT
                     GRLYCVQQ"
     gene            complement(8857..10887)
                     /locus_tag="PLF66_22505"
     CDS             complement(8857..10887)
                     /locus_tag="PLF66_22505"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HMZ66877.1"
                     /translation="MTGIQIISLDITETKTQEVKLKQSDDKYKLLYESNLMPIAVFES
                     ETLKFLSVNKAFSEKYGYTQEEFLNMTILDIRPQNTDEIPKVKESIKQNDQGVVNVGI
                     YTHKKRNGEIIEVEIIRSDVIFEGKNAKMVVVNDVTERNIIQKELDLQNLKLKELNTA
                     FNQAQKLSHVGSWQWNMATDEAEWSEEMYNIYGVTKENFYPSNANVAKTVLPEDLPKI
                     EQGITSLLNGKLFSPFEFRIRRPSGEIRHVYIVALEKKSENMLFGVTKDITERKKTEE
                     ELIQAKNLAEAANQAKSDFLANMSHEIRTPLNSVIGFTDLLLKTKLDPDQKLYLKTVY
                     QSANFLLTIVNDILDLSKIEAGKLNLKMDKVNVFSIAEQSADVIKYKAQEKGVAIRIN
                     VDKNIPIIWADPIRLRQIMLNLLSNSIKFTDLGIIEINIELKKIYSNENEIPEEAEIL
                     FSVRDTGIGISKEHIEYIFESFAQVDTSTSRKYGGTGLGLTISNRLLSLMGSKLELET
                     ELGKGSNFFFLLKAKIESQQSKQIETIQKEEMIKPKYSFMPQKKFCILIVDDDIVNRF
                     LAKTLVKSILSEADIIEAENGIDALEKFKTNKTDLILMDIQMPEMNGYEATKEIRKIE
                     PDTKVPIIAITAGTKVGDKEKCLAEGMNEYYVKPVKKEIIEVILLKWLGEIG"
BASE COUNT         3627 a         1805 c         1856 g         3694 t
ORIGIN      
        1 ttataaattc atttcttccg ctaaggatga tcctaaagaa ttagagactc ggttttctga
       61 ttgggaaaaa atttcttatt tttttacaca aaaaatctat gagaccggtg aaaaacaatt
      121 cggaaaaaat tatctcgaat taattcagaa agaaaaaaaa cgactgaaag aattttatcc
      181 gattgcaatt gaatttatag aaagtttacg tgaactagat gataaatcaa ttcgttacca
      241 ttgtttttta ttgttctctc catcgtataa tatttcatcc aggggttata tcattgattt
      301 aaatggaaat cgatttttaa cctatcgata tattcaacca aacaaaaata aaatgactat
      361 cgttggttat tcatatgaat cttacagaaa atacattcac aatttaacaa tcaattctct
      421 ctatgttttt ttaatcggat tatttttgtt tttaattgga attccttttt tactttcgag
      481 aactttatca gaaccattga aagaactttt gtctggagta aaaagagtag aagaaggaga
      541 actgaataca attgtaaaaa taaaatacca agatgaaatt ggttacttag ctcaatcttt
      601 taataaaatg gtagattcca taaaagactc taaacaaaaa ttagaagact atgctgaaaa
      661 tttggaaagt aaggtagaag aaagaactag agaattaaaa caaactctcg agcaagtaga
      721 aagtttaaaa gaataacaag atggagatta ttttcttacc actcttttac tcgaaccact
      781 cagtgtgaat gaagcaattc atacgaatat ccaagtgcaa ttttttacaa aacaaaagaa
      841 agagttttat tttaaaaaaa agaaacacca gatcggagga gacatttgca ttgcgcaggt
      901 gattcaactt aaaaataaaa aatacattgt ttttctgaat gcagatgcta tggaaaaatc
      961 aattcaaggt gcgggcggca tactagtgtt aggctctgtt tttcaatcta tcattcaaag
     1021 aactaaaaat tatcctcttg aatccgaaaa ttatcctgaa ttttggatca aaaaagcttt
     1081 caaagaattg cataaggttt tcgaaacatt tgaaggctcc atgcttgtct caatggtgct
     1141 aggactagta gaagagaaaa ctggtttagt ctattatatg aatgcagagc atcctttggt
     1201 tgtgctctac agagacggaa aagctagttt tctcaaggag cgaaaacttt ttaataaatt
     1261 gggacattta gggatggatt ctgaaatttt tgtttctacc tttcaaatgt tgccgggtga
     1321 tcagctcata attggttcgg atggaaagga tgatttcatt gtttcaaaaa ctgaattgaa
     1381 cagaaaaatt aatgacgacg aaagtatttt ccttgagaaa gttgagaaga ccaatggtga
     1441 aattcaaaaa ttgtatgaag tgattagttc tgaatttgag cttatcgatg atttttctct
     1501 tttggcaatc aactacccca atgattcaaa tgcattcaat cattcggaag aagtgattca
     1561 acaaaaaatt gcaaatgcca aatttgcatt agaaaatcca atcaataagg aagaaggtct
     1621 tttactccta gattctttgt attctgacta tccgaatcaa gaagaagtaa ataaaataat
     1681 aattgagtat catactaaac attctaacta ccaaaaggca aaagaaatat actcaaaatt
     1741 cattcaacat tctgaaagct caactgaaat actttttctt ggttctcaat tagaaaagag
     1801 gattggaaac tatgaagaat ctataaagat agcagaaagg gtaaaattgc gagatccaaa
     1861 tcatctgaaa aatttaattc atcttgctga tttgtattta tttacaaata acaaagaacg
     1921 tgctcgaaag ataattttga aaatcaaaaa aatagatccc aaacatgaaa aattaaaagt
     1981 attagaaagt aaattataaa aggcaaagaa taaattttct acgttctaag ttaggttact
     2041 attcattagt ctctactcct tcaaagaaag gtgaatatct gaatttatta aacgttgtat
     2101 gaagaggagc aaattatttc ttcatctatt ttacagcatt actattcaaa aaaatcacag
     2161 tcgattttaa tctctcatag catagcaggg gagtgattag ctaagagttt gtatgtaaaa
     2221 ctctgtaata ggtcgatagt cgctagagaa atgtaaatga tcacgaactt tggtaaatca
     2281 tagaatcaaa accatgattt atttctaaaa cttgagtttt gatgaaacaa gatcttaact
     2341 tataagtgga ttattgtcta ttgttcaacg caatagagtc tatagctaga accgcaaacg
     2401 ctattggata catagagaat ggaattagtt gttgcattgc tgtttccata atttccaaat
     2461 cctgcacttg tccagccagt acattctgct gtggaggcag cggttgtatt ccagccaagg
     2521 tctataccag tccatacgtt agtagaaata tttgaaattg gatttgtaag aggaaaagta
     2581 aatattctgt catttgtggc ctgagcaatg aaagttccat cagggcgata gtattttata
     2641 cctgcgaata aaacccaatc aattgcctca gcagaaaatc ccacacatcc acctccatta
     2701 ttacatgcct ttcgagaaac aaatccaatc atcgccttat aaattcctgg acccaaaggc
     2761 tttgcgggat ctgaattaca aaaattatcc gccgcactcg ttgttgccca gtttcccaaa
     2821 attccattcc ctgatagaaa aattcttttg ccaggaagaa ttatgttcag agtcagattt
     2881 gcagtttgcg ttcccaggct actaatagct gttacggtgt aatttccgga tgaaattaaa
     2941 aaactaggag tacctgtaat ttctccagtg gaaggattaa aatttagacc agccggtagc
     3001 gaaggggaaa tagaatagga tgtaaaagtt ccaaccactt ggggaagtat agatcctact
     3061 gaattaagtg cagtgatagt atagggggat cctgtatagg aaatggatat ggaactagaa
     3121 ttagcaccag tatagctcga gctatttact tttttatttt caattccaca aaaactagaa
     3181 gtatcatttg taaattgttt taataaatat actttctgaa aactactttt atcaaaagta
     3241 cagggagagt cgtattctgg attgttacaa gatggaaata gaaaaaagaa aaccgttaag
     3301 tagatttttg tatccacaca atactcccaa attaaaaata atttttccgt ttagactctt
     3361 ttgatttcca tcaaccttgt atttgtaaac actttttttt gctaattatt tactaatcta
     3421 atttttctgg ctatagattt acaatccctt tttctttttt ggcttacaat tgttagctga
     3481 atgagataca aaatgcaaga tcgataaaaa ttaacgataa gcgctaaaaa tgatattata
     3541 tttttaaggg tgtaattctt tgaaacgaga tatgcatata attttagcca tgagaaaggc
     3601 atattttatt tctaccggaa gatttagcag catacattgc tttgtcagca ttcgaaataa
     3661 tagaatctaa attagatacg atggagtcag tcacgccaat actcactgtg agtcgaatcg
     3721 aaacatttga tgataggtct tcagaagttt cggtaaaggt aagaattcta tttaaagaaa
     3781 tcgatgcctc ttttattgta gtgtcaggaa aaagaataat aaattcttct cctccccaac
     3841 gacaggcgat atcagtaatt cggagatgag atatcataat ttgtgagaaa gccaataata
     3901 cttcatcacc cactgaatga ccatgagagt cattgatttg tttaaaatga tctaaatcta
     3961 atattgcaat agaaaaaact cttcccgttc ttaaaaatcg atctgattca tatttaaaac
     4021 ggtttagaaa ggctcgccta gttaataatc cagttactaa atcagtattc gccatcatct
     4081 ctaatttttg attcgcagaa ataagctctt tcgttctttc tttcactttt aactctaaag
     4141 tttttcttac tgttctcagt tgttccaatg ttatcgcaat ataatgtgga acaaacccaa
     4201 tacagagtat caaaaaagaa atgttaaaaa aactaaacca atcgctgttt gttaaatttg
     4261 aacctgagat tcctaagtat aaaaatagat agccagttgt atacgttaag gatagagttg
     4321 atccagcaaa acgatacctt aacacataaa ctaatatgac aggaacaatt aaactaatca
     4381 aaggtatatg cacgtataac gctaataata caggcaataa aagagaaatt ccaattcgaa
     4441 aagataaact caaaatttcc aaagaatggt atcttcgaag tgaaaagaac aatggagtta
     4501 ctaggaatat tcctaacata tcggcactca caatttgaat tactttcaca gcatagattg
     4561 gaatcgaatc tgacattgga actttcccta tataatgcca aatcaatggt aaagaccaaa
     4621 cagtgagtat tggtggaagt aaacaaaacc ttaaaaaaaa taagaaaaag tcttttatgg
     4681 taagaaacga tattcgatag gtcttgtata aaaaataaca gatactagtt tggattaaat
     4741 ctactacagc accaagtagt cctgaataca aggcagccca tctatcctct gtcgcactga
     4801 aaaaaccata tcctccgttt gcaatactgc tagaaagata aactaatatc agtccttttt
     4861 ttccaaaaac caatatagaa acaagtccaa tcccagcagg taaccataac acagaaagag
     4921 aagagggggg gcgagaagtt aaccacatac ctataatggc aaatccgata tataggatgg
     4981 aaaaacttat atactttatt attattttta gagtatgact atcgtttgat tctgttttct
     5041 gcattggaga atactattta ctagctcacc ttacattatc attgcttaat aaaagcaatg
     5101 cttgagtttg ggggaaccaa acgttcatgc gcctagcggc gggcttgtcc acccatcgat
     5161 ttagaagctc tccagcagct aagctatcgg aggctattga atttattaat ttattggctt
     5221 tatttaatat tctaatttcc tttatcatac ttatcaattt ctatcttttg cgtaaggtta
     5281 gttactaaat tcagaattta tttttactag tttataatta tccctttcga tttacaagaa
     5341 atttcagcta gttaaataga ctaaaaaaaa agtttttaaa agatactttt ttctattcat
     5401 agatgatcac acaaatggat aatatttaaa aagtatatgc aatgagcccc tttttctaaa
     5461 ggatatagga aaaaatttaa aatcctcggg tgaattctac ttatttatca attacaaatt
     5521 tgttttaaca tagaaaattt aaatatttcc tagtgaattt ttgggctaca aaaaattcct
     5581 cctctattta aaaacatctc ttcctgagag cttctcaatt caaatgaata taagtctttc
     5641 ctttgtttga aaaaaaggag aattaaacaa aaatcggata gaaataaaaa tacaaaatcg
     5701 atagccgcga gatatatgac aaattggaaa tactttttct gtgattagag agaataatgt
     5761 tttgaattga tttgattctg taagattatc attctatttc tgaaattatt tgagattcta
     5821 gaaatgagtt tttcttttcc tctggattct ttctgacttg gagtagaaaa actttccttt
     5881 cctgaaaata aatagaaaga ttcttgaaat atgaacatgc caatatttcc aatacaaaaa
     5941 caaaagaggc ttggttctaa aaagattttt tccagttctt cccaaagata tcgaattgtt
     6001 atctattttg tatcagtgat aaatcttttt tccatcactg ttcattttaa agaattggaa
     6061 acttcaatct aggttttagt gattcacctt tcttagattt gaaatccaaa ctcttctagg
     6121 aaaaatgaga tataaaaaga aaaggataac ttggaatcaa tagatcattc cctgatattc
     6181 gttttacctt ttgaaaaaac tacgagcaat cggaaaaaag agaggagatg aaaccaaagt
     6241 aaccaaactt tttaagtgga gaatgggttt tacttttaca ctgaatagca gccgagcgaa
     6301 ttttattttt cggtctgtct tctatcgtgg aagtattctc aagttccagt tttttcttag
     6361 gtattttcct ttctttgtaa agttggattt gcccttttga attgtgtaaa attttattcc
     6421 atagaggatt ggtgattttc cgaaaggcaa gcctttaaaa aagtttggat ttactgtttt
     6481 gatctctaca tctcttctag attcaggata attcaaattt acaaattttc tatagttaat
     6541 taaaatgtat tttatacgag taacttatca ttgttatttc gtgttcattt taatggataa
     6601 aacaaaggag cttttatgaa taaagatatg tttcaacaaa tgaaatccgg aaagggtttc
     6661 atcgcagctt tagaccagag tggaggcagt actccaaagg cactcgcact ctatggtcta
     6721 aatgaatctt cctattcaaa tgaggcagag atgtatgatc gtattcatga aatgcgttca
     6781 cgtcttatta aatcgccagc gtttaacggt gatcgtatca ttggtgcgat tctttttgag
     6841 atgactatgg aacgcacaat agatggaatg ggaactgccg aatatctttg gaacaagaaa
     6901 aaagttgttc cctttctcaa agtggatagt ggtcttgcaa acgaggcgaa tgatgttcag
     6961 cttatgaaac ccattccaga actcggagcg cgtattgctg cagcaaataa acacggtgtt
     7021 tttggaacca aaatgcgttc ggtgatcaat ttagcgaatg cgacaggtat tgaagaagtg
     7081 gtaaaacaac agtttgaagt aggaaaagaa atcattgcag gtggacttgt tccaatcatt
     7141 gaaccggaag ttaatatcaa aagcccagaa aaagcagagg cagaagagat attgaagaaa
     7201 gcattactca aatatgctga tggattgaag agtgatcaga atattatgtt aaaactctcg
     7261 attcctacaa aagtaaattt atacaaagaa ttgatagagc atccacgtgt tgttcgtttt
     7321 gttgcattat ctggtggtta ttctagagag gaagcaaatc gtctgctagc tctcaatcat
     7381 ggaataattg cttccttttc tcgtgcattg acagaaggat taacagcgca acaaagtgag
     7441 agtgagttca actctgcact cgattccaca atccaaggaa tttacgacgc atctattacc
     7501 tagaatattg agggagtaat ccctcattta ttttaaaaaa ctttcttttt agaaaattca
     7561 caatgtattt tgattgaaag ctttcatttg tgaaagaatg aaagcttgga aataattggg
     7621 tttcaatttg tcgataggcg ctagaagtag aaaggaaaat aatttaggtt gaacttttag
     7681 ttaataattt ttatttacca gaaagacttt taagcccata ctggtattgt tttgaatgag
     7741 cgaattggaa tgttattata ttataagaaa aagtttctat tactgctatt agttttggtt
     7801 tcttgtcaac cagttgagta tcaaaatcca tgtgaaacaa cttcaaaatc atttcaaaaa
     7861 ctttatatta cacaaacaat tacgaacagc acacaaggtt tttgtggaat cacttcttct
     7921 aagaattcat tcggtagcaa tggagccgac agtgggaaaa attccttttt aatctcttct
     7981 ccaacgttag gttatacctt aaaaaatgga ggcaccgaca cattcacagt taaactttcc
     8041 gctcaacctt ctgcatccgt tactgtacct ctctttgttt ccaatccagc tcaggcgtct
     8101 gtatttcctt cttcgattac atttacgact gccaactgga attctcccca gacagttacg
     8161 gtggctggaa ttgatgattt gatgtatgga gtagcaggaa gttttactgt caatttgggt
     8221 ccttctagca gtcctgatct attagcacag aatataacag ggagcactgt ttcgatatca
     8281 aaccgagact tccgcaagtt gatctttgta tccaatttaa cgactggaaa tacaggcggg
     8341 atttcaggag cggatgcctt ttgtaattcg gattcaaaca aaccgatcaa tacagggatc
     8401 tataaagctt ttcttacatt gcccggagtt cgtgtcgcat ccgtcacagc aaatgcaggg
     8461 gatgggcaaa ttgactgggt tttgaaaccc aatcaattgt acactcgtcc aaatggactt
     8521 ccgattataa tcacaaattc agtttccctg tttactgcat ttccactaat aaattccgtt
     8581 tacactgctg gagatgccat ttggacagga tttcagggca caagcgactg gatttctatt
     8641 ggaggatgcg ttggctggac aagcacagga gcgtttggaa cctatgctgt gggaaatgcc
     8701 gtaaactcta cttccctctt atttggtggg aatctaagtg gcgcaaatac tggtagactg
     8761 tattgtgttc agcagtaact aaatcttttt tttgaggtag gcgttagtct tctcgtaaac
     8821 atcttcttgc tttttgtttc aactaataaa agaacgttaa cctatttctc ctaaccattt
     8881 aagaagtatt acttcaatga tttctttttt tactggtttt acataatatt cattcatacc
     8941 ttctgcgagg catttttctt tatctcccac ttttgtgcca gcggttatag caatgatagg
     9001 gacttttgta tctggttcga tttttctaat ttctttggtt gcttcgtatc cattcatttc
     9061 cggcatttga atgtccatga ggattaaatc tgtcttgttt gttttaaatt tttctaacgc
     9121 atctattcca ttttcagctt ctataatatc agcttctgat agaatacttt ttaccaaggt
     9181 ttttgcaaga aatctattta caatatcatc atccacgatt aatatacaga attttttttg
     9241 tggcataaat gaatacttag gttttatcat ttcttctttt tgtattgttt caatttgttt
     9301 ggactgttgt gattctattt ttgctttaag tagaaaaaag aaattacttc cttttcctag
     9361 ttctgtttct aactctaact ttgatcccat taaagagagt aagcgattgg aaatggttaa
     9421 tcccaagcct gttcccccat acttacggct cgtagaagta tctacttgcg caaaggattc
     9481 aaaaatatat tctatatgtt cttttgatat tccaatccct gtatcacgca cagaaaaaag
     9541 tatttctgct tcttctggta tttcattctc attagaatag atttttttga gctcaatatt
     9601 gatttcaatg attccaaggt ctgtaaattt tattgagtta ctcaagaggt taagcatgat
     9661 ttgccttaac cgaatcggat ctgcccaaat gattggaata tttttatcta cattgattcg
     9721 tatcgcaact cccttttctt gtgctttgta tttgattaca tctgcggatt gctctgctat
     9781 ggaaaaaaca tttaccttat ccattttgag atttagtttt ccagcttcta ttttggataa
     9841 atctaaaatg tcatttacaa ttgtaagaag aaaatttgca gattgataca ctgtttttaa
     9901 gtaaagtttt tggtcgggat ctaatttagt ttttaataat aaatcagtaa aaccaatcac
     9961 actattgagt ggggttctaa tttcatgact catatttgca aggaagtccg actttgcttg
    10021 gttagccgct tctgctagat tcttagcttg aatgagttct tcttcagttt tctttctctc
    10081 agtgatgtct tttgtgacac cgaataacat attttccgat tttttttcta gggctacaat
    10141 gtatacatgt cggatttcgc ctgatggtcg tctgattcta aattcaaagg gagaaaataa
    10201 tttaccattt aacaaagagg ttatgccttg ttctatttta ggtaaatctt caggtaaaac
    10261 tgttttagct acattcgcat tggatggata aaaattttct tttgtcactc cgtagatgtt
    10321 atacatttcc tccgaccatt cggcttcatc tgttgccata ttccattgcc aacttccaac
    10381 atgacttaat ttctgagctt gattgaaggc agtatttaat tctttgagtt ttaagttttg
    10441 aagatcaagt tccttttgaa ttatatttcg ctctgtaacg tcatttacca caaccatttt
    10501 ggcatttttc ccttcgaaga ttacatcact tctaataatt tcaacttcaa tgatctctcc
    10561 attgcgtttc ttatgtgtgt atataccaac attaaccaca ccctgatcat tttgtttgat
    10621 tgattccttt acttttggaa tctcatctgt attctgagga cgaatgtcta aaattgtcat
    10681 gtttagaaac tcttcctgtg tgtatccata cttctcagaa aaggctttat tgacggagag
    10741 aaattttaat gtttctgatt caaaaactgc aatcggcatt agattggatt catagaggag
    10801 cttatattta tcatcgcttt gttttaattt tacctcttgc gtttttgttt ctgttatatc
    10861 cagagatata atctgaatac ctgtcacttt gccttcatct agaattggct caaggctaga
    10921 tcgaaacctg aactactaca tatttagtgg acacgatttt aggcaaattg cttctcatat
    10981 tg
//
LOCUS       DAOQOS010000152        10928 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_3108_length_10928_cov_6.405684, whole genome shotgun sequence.
ACCESSION   DAOQOS010000152 DAOQOS010000000
VERSION     DAOQOS010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 10928)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10928)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10928
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3108_length_10928_cov_6.405684"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(131..3310)
                     /locus_tag="PLF66_22510"
     CDS             complement(131..3310)
                     /locus_tag="PLF66_22510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004768375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HMZ66878.1"
                     /translation="MIEKLIELSIRNKYIVLLLTALVSFIGIISAANLSVDAVPDVTN
                     IQVSAVTVSPGLSPMEVEQFITNPIELSLMGIPGATEIRSISRTGVSSVTVIFEDNVN
                     IWFARQLVSERLKVADKEIPPEYGTPELSPVATALGDIYEFVLKSDRHTPMELRTYID
                     WELSKKIKSIPGVIEVNSIGGEAKEYQVIIDPRNLVIHNLTLSEIYEDIKSANTNTGG
                     GYIIKGNEQVVIRGEGQFEGIDEINRIAVRTAQDGTPLLLGQIAEVKIGSALRFGIAS
                     KNQTEVVAATVIMLLGQNSRTVVSDVKKKMDEIQKTLPEGMIIEPFYDRSEFINRALS
                     TIFINLAEGAILVLLTLIVTLGTFKGGVLVAMAIPISMLCAVIFMKQLGVVGNLMSLG
                     ALDFGLLVDGSIVMLESVMAGFIAKKSLFETPMNRFQIAETTESIILENCNRVGRAAA
                     FSVAIIMLVYLPLMVLEGVEGRMFRPMAVTVALALGAALLFSITVFPASIAVIFKEPI
                     FHKSHYWEILERYYKIILHWGFKHKKRIILVSLLLIFLSMLLATTLGSEFIPRIDEGE
                     FEMDVKRLPSTSIEYSKDLNIAIERILSEIPEIKSAVSRVGRGESAAEPAGTEETSIM
                     IKLKDKKEWKNAKTREELMTLIKDKVLQNIPSSYISMSQPIENRINSLLAGSKADVVV
                     KVYGEDLETLKKIGEDLSGIMKEIPGTGDIRVQRVLGLPVVRVNTNYDLMARYGVSSS
                     EILRTVEMLRVGTNAGKIFEGLKRFDLVLRLDLNIIKDIKQINNIPVMSVTGKTIPLG
                     QVADIELIESAASIQREDLKRRLFVEVNIRGRDLVGYIEEAQEKTKIIANNLPSGYEI
                     KWGGQFENFTRAKNRLLLVVPIALGIIFGMLILAFGSIFYALGVFIVVPLATSGGIIS
                     LALRDLPFSIPAGVGFIAVSGIAVLNGVVYASALKDKLKEGLPLTEAIYASAIESLRP
                     VMTTEVIAAIGFIPMALSNMAGAEVQRPLATVVIGGVITATALSRLLLPITMEYLLSK
                     SIKIEEKKNKEIAETKSHLIALAYEEEVD"
     gene            complement(3469..4098)
                     /locus_tag="PLF66_22515"
     CDS             complement(3469..4098)
                     /locus_tag="PLF66_22515"
                     /inference="COORDINATES: protein motif:HMM:NF018264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrsF family protein"
                     /protein_id="HMZ66879.1"
                     /translation="MKTEKLIENLVKDSSSVKPILSTPIVFLFWLLASLSFLILFVWS
                     RSNELNTLHSPEKSYEIIPILISIFFSGYASIHLSIPGNRLRPIFLSIPFFLFLIWFL
                     LIYSKYLLSPESPKLDFEFHGCIQDLLWMTIPSFIILFYIINKRIPINKTWIGYLLLT
                     ASSSIGAIGVLLLCPDETFYHLVKVHFSPIFTLSLSGFLIGKIILKHPI"
     gene            complement(4088..4636)
                     /locus_tag="PLF66_22520"
     CDS             complement(4088..4636)
                     /locus_tag="PLF66_22520"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HMZ66880.1"
                     /translation="MIEQDLLNQLMQEAQFGNSKSYYTLLNKIKTILEKYLSRRIYSH
                     EDREDVLQDILIAIHYSKHTYLPNRPFYPWMYAIAKNVLSKYYKKIQKLKDISFDMDI
                     LETQSTDPILEESDSAELMKNIHSAIEELPTRQRQILYMMKFDKMSIKEIALKLKLSE
                     GNVKVIAHRSYQRIRKRLKNED"
     gene            complement(4740..5258)
                     /locus_tag="PLF66_22525"
     CDS             complement(4740..5258)
                     /locus_tag="PLF66_22525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66881.1"
                     /translation="MRTNTILYSNVIHQLTEHLAKISDNAYIDRLTMFGGSTLGEHVR
                     HIYNFPECLINGIKNGIVNYEKRFRNPKIEENRVFAIESILEQVNLISELAVYPDKPL
                     LLEIGLKGDNQIQTNFARELHYITEHTIHHMAIIRIGIEYLCPTLQVEPSFGVASSTI
                     EYKKKLTHNFSE"
     gene            complement(5260..5682)
                     /gene="msrB"
                     /locus_tag="PLF66_22530"
     CDS             complement(5260..5682)
                     /gene="msrB"
                     /locus_tag="PLF66_22530"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020780808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HMZ66882.1"
                     /translation="MGEQKTKQTYSIQKSDEEWKKILSPEAYKVLREKGTERPFTNEF
                     SLKEKGYFVCAACGNKVFASDHKFDSGTGWPSFYQPINKENVGEETDKSWGMTRTEVH
                     CSKCGGHLGHVFNDGPNPTGLRYCINGIALKFIKFDSK"
     gene            complement(5881..6282)
                     /locus_tag="PLF66_22535"
     CDS             complement(5881..6282)
                     /locus_tag="PLF66_22535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002773253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HMZ66883.1"
                     /translation="MKIKFIWMFRIIASVIMLQTLFYKFTGSPESIYIFTTIGVEPFG
                     RIAAGISELIASILLLFSSTYLYGAILTLAIMSGALISHLTILGIEVQKDGGLLFLLA
                     IVVFITSLGIILLEKEKFIFLMQTIRSKFKK"
     gene            6404..7612
                     /locus_tag="PLF66_22540"
     CDS             6404..7612
                     /locus_tag="PLF66_22540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010569082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HMZ66884.1"
                     /translation="MKQKLISILVLLIGDFKKNSLEHRLFNSIALANGIINIFGSFGT
                     FYLPNFLILFSLNFGTGIVFLILYYLARFKSIYYILYWPLNLTILFFLAANWFTNGGS
                     LGGSHYYLIPALVIATILVRNNNIIFVYAVYSILTLALFGVEYYYPQLITMFDKNQDR
                     YLDASINYAFVQLFTGFLIFILSRNLDLERKKSDKLLLNILPEKIANELKQNDKVVPA
                     RYTSVSVLFTDMAGFTKIAENMSPEELLFELDTIFSVFDSIVKKYGVEKIKTIGDAYM
                     AAGGIPIANETNAVDTVLCALEFREYMNRMKIKKQEEGKPFFELRIGIHTGSLVAGVI
                     GHEKIVYDIWGDTVNTASRMESSGLIGEVNISSATYYLIKDQFDCEYRGKISAKNKGE
                     IDMYLVKGKK"
     gene            7609..8469
                     /locus_tag="PLF66_22545"
     CDS             7609..8469
                     /locus_tag="PLF66_22545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HMZ66885.1"
                     /translation="MNLVYRSFYKYFSYTRYQILKKELGLVESYEDIGGNEVCYFVSQ
                     NTGKPLILIHGLLDSAFGFRKLVPYLDKQYKLYIIDIPGFGKSRLPKIKYLYQIDIFA
                     KMIYRVIENLNLENAILCGHSMGGLISQHIALLDLHKKRISKLFLLSSGGIPHPKRDE
                     MRAILFPSEHEEVTRLLQHLYYREAPAPSKIIRKTLVVAWNSIEYQYLAENTIEREHE
                     IFFGKEARKIKIPTIIISGKQDSITSPESMKILKSYIRGSKLILLDHARHAIHLEKPR
                     EIAEILNNSH"
     gene            8999..9613
                     /locus_tag="PLF66_22550"
     CDS             8999..9613
                     /locus_tag="PLF66_22550"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HMZ66886.1"
                     /translation="MDKTILEIEKTFRPDYEERDFDGLTSLHIAANEGNSEKVIALLN
                     NGSDINAVTNYWHTPLHYAVNRGGYEIVKILLERGAKVNVLSNFDYTPLHFAANHGKL
                     REASLLIEHGAPLNIQNTFGHTPLHCAVNHTRIFLECDDCVERGKYKYCWGVHGRIEV
                     AKLLIEKGARMHLEDGDGNTPLYLAIKNGYPEVVESIHVKLKIV"
     gene            complement(9621..10550)
                     /locus_tag="PLF66_22555"
     CDS             complement(9621..10550)
                     /locus_tag="PLF66_22555"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HMZ66887.1"
                     /translation="MQDIKNILTQNKNNLKNWIEENQIQAYRIYDRELENYPVAIDLY
                     LNKAHIQVYESKNTEANDDLLIKNISDDVEIALGIPKINQYYKSRKRQKEGGQYEKVA
                     TTKERFIIKENGADFYVNLKDYLDTGIFLDHRKTRTLVKNDLAKGKERMLNLFSYTSS
                     FSIVSALAGVKYTTSVDMSNTYCDWSRDNFKLNSLEKFNHIAIRDNVLFFLENMKKNW
                     MFDLIVIDPPTFSRSKKMPVPFDVQKDHPFLINKSLEHLNPDGIILFSNNFQKFRLNG
                     DLLNSKTIFDITKDTIPVDFKNQKIHNCFLIKK"
BASE COUNT         3629 a         1772 c         1800 g         3727 t
ORIGIN      
        1 ggaacacgaa gttaggttag aagtaaaaag taaaatcctc cgtgcttatt ccacccttcg
       61 ctccaagtat tcccgaccag cgccttcgca tgtgtgcttc gtggttaaaa attcttaaat
      121 cgaattgaga ttaatctact tcttcttcgt aagcaagtgc aatcagatgc gattttgttt
      181 ctgcgatttc tttatttttc ttttcttcaa ttttaattga tttagataat agatattcca
      241 tcgtaatagg aagaagcaat ctagaaagtg cagtagctgt gatcactcca ccaatcacaa
      301 cagttgcaag tggtctttgg acttcggctc ctgccatatt ggacaatgcc attggaataa
      361 aaccgatagc tgctatcact tccgttgtca tcactggtct taatgattcg atcgcagagg
      421 catagatcgc ttctgttaaa ggaagtccct ctttcaattt gtcttttagt gcggatgcgt
      481 agaccactcc atttaacact gcgattccac tcacagcaat aaatccaacg cctgccggaa
      541 tactaaaagg taaatctcta agagcaagac ttatgattcc gccagaggta gccaaaggaa
      601 ctacaatgaa tacacctaga gcataaaaaa tactaccaaa ggctaatatt agcattccaa
      661 aaataattcc aagtgcgata ggaacaacta gaagtaatct gttttttgct cttgtgaaat
      721 tttcaaattg accaccccat ttgatttcat aaccggaagg caaattgttt gcgattattt
      781 tcgttttttc ttgcgcttct tctatatagc ctactaaatc tctaccgcga atgtttactt
      841 ctacaaaaag tcttcgcttt agatcttctc tttgaatcga tgctgcggat tcgatcagtt
      901 ctatatcggc aacttgtccg agtggaattg ttttacctgt aaccgacata acaggaatat
      961 tattgatttg ctttatatct tttattatat ttagatcgag tcttagaaca agatcaaaac
     1021 gtttgagtcc ctcaaaaatt tttcctgcgt tagttcctac acgaagcatt tctacagttc
     1081 gtaatatttc agaagaagag actccgtatc gagccattaa gtcgtagttt gtatttactc
     1141 gcacaactgg taaacctaga acccgttgca ctcgaatatc tcctgttccg ggaatttctt
     1201 tcattatacc ggaaagatct tctccaatct ttttgagcgt ttccaaatct tctccgtaaa
     1261 ctttgacaac cacatcagcc ttggagcctg ccaagaggga gttgattcga ttttcaatcg
     1321 gttgagacat gcttatatag ctagaaggaa tattttgtag aaccttgtct ttaattaaag
     1381 tcataagttc ttctctagtt tttgcgttct tccattcttt tttgtctttt aatttaatca
     1441 tgatacttgt ttcttctgtt cctgctggtt ctgctgctga ttcacctcta cccacacgag
     1501 aaaccgcact tttaatttct ggaatttcac ttagaattct ttcgattgca atatttagat
     1561 ccttagaata ttcaatagat gtagaaggaa ggcgtttaac gtccatttcg aattctccct
     1621 catcgatacg aggaataaac tcggagccta atgttgtagc aagtaacata gaaagaaaaa
     1681 tgagaagaag agagacgagt atgattcttt ttttatgttt aaatccccag tgtaaaataa
     1741 ttttataata cctttccaaa atctcccaat aatgactttt gtgaaaaatt ggttccttga
     1801 aaataactgc tatacttgcc ggaaaaacag ttatagaaaa aagaagagca gctcctagtg
     1861 ctaatgcaac cgtaactgcc ataggtctaa acattctacc ttcaacgcct tctaaaacca
     1921 tcagaggtaa ataaacgagc ataataattg ctacagaaaa tgccgcggcg cgaccaacac
     1981 ggttgcaatt ttctaatata attgattcag ttgtttctgc aatttgaaat cgattcattg
     2041 gtgtttcgaa taacgatttc ttagcgataa agcctgccat tacggactct agcattacaa
     2101 tggaaccatc aactaagagt ccaaagtcca gtgcgcctag actcattaaa tttccaacaa
     2161 ctccaagttg tttcataaaa ataactgcac aaagcatcga aatcggaata gccatcgcaa
     2221 ctaagacacc acctttaaaa gttccaagtg tcacgatcaa ggttagtaaa actagtattg
     2281 ctccttctgc tagattaata aaaattgtag agagagctct gttgataaat tcagatctat
     2341 cataaaaagg ttcaattatc attccctctg gaagagtttt ttgaatttca tccattttct
     2401 tttttacatc tgagacaacg gttctagaat tttgaccaag tagcataatg acagtagccg
     2461 caaccacttc agtttgattt ttactagcga ttccaaatct tagagcagat ccgattttta
     2521 cttccgcaat ttgaccgagg agtagtgggg ttccatcttg agcggttcta acggctattc
     2581 gattgatttc atcaattccc tcgaattgtc cttcgcctct aattactact tgttcatttc
     2641 cttttatgat gtagccgccg cctgtatttg tatttgctga tttaatatcc tcatatattt
     2701 cagataacgt taaattatga atcactaaat ttcttgggtc aatgatgact tgatactctt
     2761 ttgcctctcc tccgattgaa tttacttcaa tgacacccgg gatggatttt atttttttgg
     2821 ataactccca atcgatatag gttctaagtt ccattggcgt atgacggtct gattttagaa
     2881 caaactcata gatatctccg agtgcagttg caactgggga taattcaggc gttccatatt
     2941 cagggggaat ttctttgtca gctactttta atctttcgct aacgagctgt cttgcaaacc
     3001 agatatttac attatcctca aagatgactg taacactgct gacaccggtt ctagatatgg
     3061 aacgaatttc tgttgctcca ggaattccca tcagagacaa ttcgattgga ttagtgataa
     3121 attgctctac ctccattgga gagagtcctg gagatactgt aacagctgat acttggatgt
     3181 ttgtgacatc tggaactgca tccacagaaa gatttgcagc gctaatgatt ccaatgaaag
     3241 atacgagtgc tgttaataaa agaactatat acttatttcg aatggaaagt tctattaatt
     3301 tttctatcat ctattctgag tgctttcaac aatattgatc tattatgtat caatttcttt
     3361 agtgtaaaaa aagtcaatcg gtctaaagag ggaacctaaa ttttctagct ttcgtctttg
     3421 tgattcaagc aatcattttg caaataggat agtagatgtc agtaaaaatt aaattggatg
     3481 ttttaaaatt attttaccga ttaaaaaacc agagagactc aaggtgaaaa taggagaaaa
     3541 atgaaccttt acaagatgat agaaagtttc atcaggacaa aggagtagaa ctccaatcgc
     3601 tccgattgag ctagatgcgg ttaatagtaa atagccaatc caagttttat tgattggaat
     3661 tctcttgtta ataatataaa acaaaataat aaaagaagga attgtcatcc aaagtaaatc
     3721 ctgtatgcaa ccatgaaatt caaaatctaa ctttgggctt tctggactaa gtaggtattt
     3781 tgaataaatc aacaagaacc aaatcaaaaa caaaaaaaag ggaatggata aaaaaattgg
     3841 ccttaaacga tttccaggaa tacttaaatg aatcgaagca taaccagaaa aaaaaatact
     3901 gattagaatc ggaatgattt cataagattt ttcaggagaa tgtaaagtat ttaattcatt
     3961 tgatctagac caaacaaaga gaattaaaaa agaaagagag gcgagaagcc aaaataaaaa
     4021 aacgataggc gtagagagaa taggctttac ggaagatgaa tcttttacaa ggttttcaat
     4081 tagtttttca gtcttcattt tttagtctct ttcgaatcct ttgataactg cgatgtgcga
     4141 tcacttttac attcccttcc gataatttta attttaatgc aatttcttta atagacatct
     4201 tatcaaattt catcatgtat aaaatttgtc tttgtctagt aggtaattct tctattgcac
     4261 tatgaatatt tttcattagc tctgcactat cgctttcttc taaaatagga tcagtggatt
     4321 gtgtttctaa aatatccata tcaaaactaa tatcttttag tttttgaatt tttttatagt
     4381 atttacttaa aacatttttt gctattgcat acatccaagg ataaaaagga cgatttggga
     4441 gataggtatg tttagaataa tggattgcga ttaaaatatc ttgcaataca tcctctctat
     4501 cttcatggga atagattcgt ctagataaat atttttcgag gatggtttta attttattta
     4561 ataaagtata ataagatttg gaatttccaa attgagcttc ttgcatcaat tgattgagta
     4621 aatcttgttc tatcatacga tatttgaaaa aatatacaat atctacttct ttatatgata
     4681 gtaaatgtag aaagtaaaaa aaattaaaat cacaaacatt tactatataa aacattccct
     4741 cactcgctaa aattatgtgt taatttcttt ttatactcta tcgtagaaga tgctacacca
     4801 aaagaaggtt ccacttgtaa agttggacaa agatactcga taccgattcg aatgattgcc
     4861 atatgatgaa ttgtatgctc tgttatataa tgaagttctc ttgcaaaatt tgtttgaatt
     4921 tggttatcac ctttcaatcc gatttctaag agtagcggct tatctgggta aaccgcaagt
     4981 tcagaaatca gatttacttg ctccaaaata gattcaatcg caaaaactct attctcttct
     5041 attttggggt ttctaaatct tttttcataa ttcacaattc catttttaat tccattgatt
     5101 agacattctg gaaaattata aatatgtcga acatgttctc ctaatgtaga gcctccaaac
     5161 attgtgagtc gatcaatata agcattgtct gaaatttttg ctaaatgctc agttagttga
     5221 tgaataacgt tactataaag aatcgtatta gttctcatat tacttactat caaacttaat
     5281 aaacttcaat gcgattccat ttatacagta tcgaagacct gtaggattag gaccgtcatt
     5341 aaaaacatga cctaaatgtc ctccacattt gctgcaatga acttctgttc tagtcattcc
     5401 ccaactttta tctgtttctt ctcctacatt ttctttattg attggttgat agaagctagg
     5461 ccagcctgtg cctgaatcaa atttatggtc agaggcaaac actttgttgc cgcaggcagc
     5521 acaaacaaag taaccctttt cttttaaaga aaattcgttt gtgaaaggtc gttctgttcc
     5581 tttttctctt aagactttgt atgcttctgg acttagaatt tttttccatt cctcatctga
     5641 tttctggatt gaataggttt gctttgtttt ttgttctcca atgattttac cacatgtaaa
     5701 aatggaaaaa ccaatcagga cacaaatgat atatttaaat attttgttca tacttaactc
     5761 caatttctct agaatattaa taaaattact tttcatcttt ttgattcgct ttacatcttc
     5821 cttttttgta taaagacagt ttttaattta ttcttgctct cagttcgttt ctcaaaaaat
     5881 ttatttctta aatttagatc gaatcgtttg cattagaaag ataaatttct ctttttctaa
     5941 taaaataatt ccgagggaag ttataaatac gactatcgct aataaaaaaa gaagtccgcc
     6001 atctttttga acttcaatgc ctaaaatcgt tagatgactt attaatgcac ccgacataat
     6061 tgccaatgta agaattgctc cataaagata agtggaagag aatagaagta aaattgaagc
     6121 aattaactct gatatgcctg cagcgattcg accaaaaggc tctactccaa ttgtagtaaa
     6181 aatatagatt gattcgggac tacccgtaaa tttataaaat aaagtttgaa gcataataac
     6241 tgaggcgata attctaaaca tccaaatgaa tttaattttc ataaaatatc tctatattcc
     6301 atttgttcgc taaatgaaat aagttacaat ttttttattt gagattcgtt tttttttaga
     6361 aaatgagttg tagtttagga gaattaggga aaatgaaatt ggtatgaaac aaaaactaat
     6421 ttcgatttta gttttactca ttggtgattt taaaaaaaac tctctagagc atagactttt
     6481 taattcaatt gcacttgcaa atgggattat taatattttt ggatcttttg gaacctttta
     6541 tctccctaat tttttaattc tattttctct aaactttgga actggaattg tatttttaat
     6601 tctctattat ttagctaggt ttaaaagtat ttattatatt ttatattggc cgctcaattt
     6661 aacaattctg ttttttcttg cagccaattg gtttacgaat ggaggctcac ttgggggttc
     6721 tcattactat ttaatacctg ctttagtgat cgctacaata cttgtaagaa ataataatat
     6781 tatttttgta tatgcggtat attcgatatt aacccttgct ctctttgggg tagaatacta
     6841 ttatccgcaa ctaattacta tgttcgataa gaatcaagat aggtatttag atgcttccat
     6901 taattatgca ttcgttcagt tattcacagg atttttaatt tttatacttt ctagaaattt
     6961 agatttagag agaaagaagt cagataagct tctattgaat attttacctg agaaaattgc
     7021 caatgagtta aagcaaaatg ataaagtagt tccagctaga tatacgagtg tttccgtttt
     7081 atttacagat atggcaggat ttacaaaaat tgcagagaat atgagtccag aagagttatt
     7141 atttgaactt gatactattt tctctgtatt tgattctatt gtaaaaaagt atggagtaga
     7201 aaaaataaaa acaatcgggg atgcctatat ggcggcaggc ggaataccaa ttgcgaatga
     7261 aacaaatgca gttgatacag tattatgcgc acttgaattt agagaatata tgaacagaat
     7321 gaaaatcaaa aaacaagaag agggaaaacc attttttgaa ctaagaattg gaattcatac
     7381 aggaagtctt gtggcgggtg tgattggtca tgaaaaaatt gtttatgata tttggggaga
     7441 tactgtaaat acagcaagta gaatggaatc ttctggtttg attggggaag ttaatatttc
     7501 ctccgcaacg tattatttga taaaggatca atttgattgt gaataccgcg gaaagatctc
     7561 agcaaagaac aaaggggaaa ttgacatgta tttggtaaaa ggtaagaaat gaatctagtc
     7621 tatcgttcat tctataaata ttttagttat acacgttatc aaatcttaaa aaaagaacta
     7681 ggattagttg aatcttatga agatatcggg gggaacgaag tttgctattt tgtttctcaa
     7741 aataccggaa agcctttgat cctaattcat ggattacttg attccgcatt cggctttaga
     7801 aagttagttc cctacttaga taaacaatat aagctctata ttatagatat tcctggtttt
     7861 gggaaaagcc gtctacctaa aataaaatat ctatatcaaa ttgatatatt tgcaaaaatg
     7921 atctatcgag taatcgaaaa tttgaattta gaaaatgcaa tcttatgcgg acattctatg
     7981 ggaggcttga tttcccaaca cattgcactt ttagatttac ataaaaaaag aatttctaaa
     8041 ttatttttgt tatcatctgg tggaattcct catcccaaaa gagatgaaat gagagctata
     8101 cttttccctt ctgagcatga agaagtgaca agacttttac agcatttata ctatagagag
     8161 gcaccagctc cttcaaagat aattagaaaa acgttagtcg ttgcttggaa tagtatagag
     8221 tatcaatatt tagcagaaaa tacgatagaa agagaacatg aaatattttt tggaaaagaa
     8281 gcacgaaaga ttaaaattcc aacaattatt atttcaggga aacaggattc catcacaagt
     8341 ccggaatcaa tgaagatatt aaagtcctat ataagaggga gtaaactgat tctactcgat
     8401 catgcaagac atgctattca cttagagaag ccaagagaaa tagcggaaat attaaataac
     8461 tcacattaat aaatgaaata aacgttccca ttaggttcac ctctattctg attgagaaat
     8521 agtttttgga atagagaata ataactgtta aacgaatatc tggggctaga gttttgacaa
     8581 atacttacga tattattcct agagcgattt gaatgatttg ttttgagttt aagaagcaaa
     8641 tttatctaaa ggaatcgata ttgcaaataa tcgaattcaa gaaagaatta tagtaaaata
     8701 aaaaagtata ttaaaaaaga caatgcttga gagctaattt cctatttttc catctgaatt
     8761 ttaatcatat ataacaaaag attgactttt attaatggta gaatctaaaa aaaataaaaa
     8821 ataaaaaaag attgcattta ttattgccaa atcggcaaac tggtgttaga tgaaaattaa
     8881 aagtcaataa ataaatcatc cattttctga ctaaccgaaa tcctcgatgc gaattcgaaa
     8941 atataaacaa agttcagagc aatacttctg aaccatcaat aaatacgagg ataacacaat
     9001 ggataaaacg atattagaaa tagaaaaaac ttttagacca gactatgaag aaagagactt
     9061 tgatggattg acgtctctcc atattgcagc gaatgaagga aatagcgaga aagtaattgc
     9121 cttactgaat aatggttctg atataaatgc ggttacaaat tattggcaca ctccacttca
     9181 ctatgctgtt aatagaggag gctatgaaat tgtaaaaata cttttagaga ggggtgcaaa
     9241 ggtaaatgtt ttaagtaatt tcgactatac accactgcat tttgctgcaa atcatggaaa
     9301 gttgagagaa gcgagtttac tgattgaaca tggtgctcca ttaaatatac aaaatacgtt
     9361 tgggcatact ccgctacatt gtgcagtgaa tcatactcgt atttttcttg aatgtgatga
     9421 ttgtgtagaa agaggaaaat acaagtattg ttggggagtt catggaagaa tcgaagtagc
     9481 aaagctttta attgagaaag gagcaaggat gcacttggaa gatggtgatg ggaatactcc
     9541 tctctatctt gcaattaaaa acggttatcc ggaagttgta gaaagcattc atgtgaaatt
     9601 aaaaatagtt taaatttatt ttattttttt attaggaaac aattgtggat tttttgattt
     9661 ttaaaatcca ctggaattgt atctttagta atgtcaaata tcgttttact atttagtaaa
     9721 tcgccgttta atctaaattt ttgaaaatta tttgaaaaga ggatgatccc atccggattt
     9781 aaatgttcta aacttttatt aattaaaaaa ggatgatctt tttgcacatc gaacggaact
     9841 ggcatttttt ttgatcttga aaaagtagga ggatcgatta caatcagatc aaacatccag
     9901 ttttttttca tattctctag gaaaaataaa acgttatctc ttatagcaat atgattaaat
     9961 ttttctagag aattcaattt aaaattatca cgtgaccaat cacaatatgt attagacata
    10021 tccacggatg ttgtatattt aaccccagcc aaagcggata cgatactaaa ggaagatgta
    10081 taagaaaata aatttaacat tctttccttg cctttggcta aatcattttt tacgagagtt
    10141 cttgtctttc ggtgatctaa aaaaattcct gtatctaaat agtcttttaa attaacatag
    10201 aaatctgctc cattctcctt gataataaac ctttccttcg ttgtagcaac tttctcgtat
    10261 tgaccgcctt ctttttgtct ctttctagat ttatagtatt gatttatttt tggaataccg
    10321 agtgctattt ctacgtcatc cgaaatgttt ttaataagta aatcatcatt agcctcagta
    10381 tttttacttt catatacttg tatgtgtgct ttatttaagt aaaggtctat tgcaactggg
    10441 taattctcta attcacgatc ataaattcga taggcttgga tttgattttc ttcgatccaa
    10501 ttttttaggt tatttttatt ttgggtgagt atatttttta tatcttgcat tctatgaatt
    10561 ctacaatatt gacatataaa aaagatagga aaactttaat actaaaaaat atactcatgc
    10621 ttgactgaca ttgaatcaaa aagagttcta aatcggcaga aacccctttg ttaatttgat
    10681 ctttttgcat cgtgaagttg aagcagagta tctcatttta ctctttttat aaaaaggttg
    10741 ttagtgaaat tttcgcattt tgtttcggtt tattcccatt aaaaaatatt tagccgaccc
    10801 gtgacccttg gaaagaccgg tccgtttata aaagtctgaa catagtcata aacgatctct
    10861 gcatcaatac ccaaatcttt ttttatgata gcagcactag caggactatc acctgaaaat
    10921 tcgctagg
//
LOCUS       DAOQOS010000153        10910 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_3114_length_10910_cov_6.309627, whole genome shotgun sequence.
ACCESSION   DAOQOS010000153 DAOQOS010000000
VERSION     DAOQOS010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 10910)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10910)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10910
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3114_length_10910_cov_6.309627"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            224..358
                     /locus_tag="PLF66_22560"
     CDS             224..358
                     /locus_tag="PLF66_22560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66888.1"
                     /translation="MESEKEYSVVNGLYSDKERVTGLNPLIQDLLNKYFALTITEIYK
                     "
     gene            complement(601..972)
                     /locus_tag="PLF66_22565"
     CDS             complement(601..972)
                     /locus_tag="PLF66_22565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66889.1"
                     /translation="MKNSLYIILILSFFSFLQCGKTIIKVNNPTRVIDPVEEPDYKKR
                     YITNFDGFQEYSRPVTPNKPQSFRSTWFTTKAELEKHKTPVCEKDVDAIILYHDFVDS
                     IIHFWVGGITSSRSILVYCTK"
     gene            complement(987..1274)
                     /locus_tag="PLF66_22570"
     CDS             complement(987..1274)
                     /locus_tag="PLF66_22570"
                     /inference="COORDINATES: protein motif:HMM:NF018045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66890.1"
                     /translation="MKKIILILFLFSLVACHHNRVKLEKEGGGTKGEVKMLYHKYWVG
                     GMFPRKIEIDGTQLCPSGIAEIDQYYRWQDAVLTQLTFGIYVPRTMKITCN"
     gene            complement(1288..1785)
                     /locus_tag="PLF66_22575"
     CDS             complement(1288..1785)
                     /locus_tag="PLF66_22575"
                     /inference="COORDINATES: protein motif:HMM:NF014427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HMZ66891.1"
                     /translation="MKSTKSIFILSILFLFISCTAWPALIPPPPASKKTSIYPIPLPG
                     QTTSTIPEVRITDIGSTTALTSVKLAFTNTKIVRAIDKNGTDISSAITWTSSNSTVAT
                     VSATGQCTITATSTAGSVTITARHASLGSATLPVDVSATGQSVLAFDGKQFLEFSTIP
                     NNNSN"
     gene            2445..3365
                     /locus_tag="PLF66_22580"
     CDS             2445..3365
                     /locus_tag="PLF66_22580"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HMZ66892.1"
                     /translation="MSLPKNKTKSSRALIVEGGGMRGAFAGGVLSAMCKYYPVDNYDL
                     VVGVSAGSCSLAYYTVEETNELISKQTILDIWKHELNGSKFISLLNYFKGKKPLDHKY
                     LIDYLLAQKYRLKSENLHKNDTVPFYVVVSNLHTLLPEYVRASKDNVLQLLRAATSLP
                     IATRGKEWLDQKLYSDGGVMDPIPIKAVIAAGYKDITVVLNKSRDHLSEPVGKLVSFL
                     SFPTLPKVAEFLREKHHLMYNEAKHIINNPPKGIKIKILDPKENIDVGLTTTNKKSLI
                     ETVNHGWEVAYRMFNTTRQRTKSFRRKIDM"
     gene            complement(3376..3681)
                     /locus_tag="PLF66_22585"
     CDS             complement(3376..3681)
                     /locus_tag="PLF66_22585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020768055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HMZ66893.1"
                     /translation="MFKSEVEVVNGVCTISLNGNISLKNAIQLKELIMKQVDNGILDI
                     VLDFGENVYIDSSGIGAIFNSQKYVNEKNGILKIKNIGHETMTILKIANLDKHLNII"
     gene            3804..4757
                     /locus_tag="PLF66_22590"
     CDS             3804..4757
                     /locus_tag="PLF66_22590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011670088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HMZ66894.1"
                     /translation="MKIKFWGVRGSIASPISGKAIKAKVEKILSMATPSDIQSEDSIR
                     NFLDTLNFSILNTYGGNTTCLEIRDSNNNLYIIDGGTGLRELGNSILMEGFLEGKGKA
                     NWIFTHTHWDHLQGVPFFVPLYIPGNRFDIYTSIDGIEERIRYQHRNTHFPVPYEALS
                     ATKNYFFVQEGSPVTINNITLTSKSVRHPGGSYSYKMVEGDKTFIFASDAEFNIDEME
                     NIDLYIEYFRGADVLVFDTQYTFDESLKKIDWGHSSASIATDIALKAGVKQLVMFHHD
                     PLYDDDKLDAVYLRALNYKEMFDKNNELDIIMAYEGLEIEI"
     gene            5005..6111
                     /locus_tag="PLF66_22595"
     CDS             5005..6111
                     /locus_tag="PLF66_22595"
                     /inference="COORDINATES: protein motif:HMM:NF015755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HMZ66895.1"
                     /translation="MTLILSGSILLGKRYFNRTKIDKKLGKSNPIHFLVVSANEQKDI
                     VFSLFVALYPTEKKVALFFLNPLISFDEQKVEKLGSEAENILVKELEKITDRKIDYKL
                     SIPEKNFYRIIDVIGGLPVYFDPLLLRKTSDSYERKVGEYNLSGEELSDFLKLKNQDN
                     PTDYLERLWCQETVALILYDHLRVIQKNIRKEWVLLFSTFFDTDLSSEEFISLLTYIQ
                     ESHLVLYVSEMPAEPITITDGKNKKVQLKVKEEVASIAFSKFVSDLRSEDFADGEFAR
                     TEVLNGTEINGLAKSVKSLLNDKRIKVLSTDNAWTLTQENTIIIDRSGNPEYSYKIAD
                     VLGVKGIKHIIDKEVGLDTTIILGEDFEIKPGKK"
     gene            6236..6637
                     /gene="rsfS"
                     /locus_tag="PLF66_22600"
     CDS             6236..6637
                     /gene="rsfS"
                     /locus_tag="PLF66_22600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002760619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HMZ66896.1"
                     /translation="MPLKEKKATTKPKEKSLSTETKKILKEIISILKEKKCEDIIVMN
                     LEEVNTYLSLFVICTVASHTQGRAAARDLEKKMKHYKLGVGNTEKKNIPTESGWTLLD
                     LGEIVVHIMTAETRAYYDLDKLWGDAKRITI"
     gene            complement(6640..6963)
                     /locus_tag="PLF66_22605"
     CDS             complement(6640..6963)
                     /locus_tag="PLF66_22605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002074700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HMZ66897.1"
                     /translation="MEELRNDLEKAIYQAILPIEDPELFISIMDLGLVYDIKVNEENI
                     ANIKMTFTSMACPAGPSMKSQVLSAAAQVDGIKDVNVEVVWNPKWDPRIMASEDAKMQ
                     LGIYD"
     gene            complement(6966..7391)
                     /locus_tag="PLF66_22610"
     CDS             complement(6966..7391)
                     /locus_tag="PLF66_22610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008593422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HMZ66898.1"
                     /translation="MSLSDELYKEVILDHFENPRNYYVLANPNVRERGVNPLCGDEFE
                     LFLSFDGDKIKEASFQGKGCSISQASGSMMTELIQGKTKEEAFTLIEKFKGMILEDKD
                     PNYSDEEADLEALNGVKKYPVRVKCAVLCWNTLDKALKG"
     gene            complement(7375..8616)
                     /locus_tag="PLF66_22615"
     CDS             complement(7375..8616)
                     /locus_tag="PLF66_22615"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017853437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HMZ66899.1"
                     /translation="MSLNPYRLKTDFPILNTTMNGKPLVFLDSAASSQKPFQVIDAID
                     KYYKEENANIHRGVYYLSQHATELYEKTRIKVADFIGARCAKIAIYTRNATESINLVA
                     QTWGRANINRGDEIVLNELEHHSNIVPWQMLALEKGASLKYIPLNEDSTLDLSDIDSI
                     ITYKTKLVVCSQMSNATGTIHEIKPLIEKAHSVGALFLLDGAQGASHIKTNVKELDVD
                     FYVFSAHKMLGPTGVGILYGKEEILERMPPWMGGGDMISEVTKEWTTYADLPARLEAG
                     TPHIAGGIAFSHAMDYLNSVGMENIHNHEVELVKYAIVRLEDLGGITLYGTKDMNHRG
                     GVVSFNVEGVHAHDVGSILDEQGIAIRVGHHCCQPYMKKMGIAGTCRASFYLYNTKED
                     VDALVEGLKKVMEIFARVVKR"
     gene            complement(8618..8926)
                     /locus_tag="PLF66_22620"
     CDS             complement(8618..8926)
                     /locus_tag="PLF66_22620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010414429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme iron oxygenase ferredoxin subunit"
                     /protein_id="HMZ66900.1"
                     /translation="MYKKLINVAEVKEGLIKVVKTKYANIAITKLGEDFIAFEDVCTH
                     DGEEISSGKIESGAIICPRHFAKFDLKNGKALCMPATEDLVTFPVRISDNMIEVDIED
                     "
     gene            complement(8937..10154)
                     /gene="sufD"
                     /locus_tag="PLF66_22625"
     CDS             complement(8937..10154)
                     /gene="sufD"
                     /locus_tag="PLF66_22625"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HMZ66901.1"
                     /translation="MEVATANKTFLSSQFDSFLSNRKEPDWLIQLRKKAFAILENTPL
                     PNSKQESWRKINLSNFSIETLLSANKKSSSFSINMDEIHSNDFSKLEEMDIQFIQDSF
                     QKLLDRYKANYFALLNFSFFDIGILIKVPKNKIIESYIEMDVQYPQGETSFQPLLFWK
                     IESNTSVKIVEKFNSPITEDFNLINPFSYIELSENSQTKFITIEDFESSTFHFRNIES
                     NQGRASALNLFHFYLGGYKGKTLINHDLSGEGANLISHGATTLAKKEFQDIEFNISHF
                     SSRTDSELKLKAVVKDKSHHVFTGNLFIPKTSLHVTASQINNNLTMARTARAESMPKL
                     EVFADDVKCSHGATVGEVNEDQLFYLMSRGLTENESRFLIIEGFLKEILDLVDKEEIQ
                     SYLVSRLTKKLYN"
     gene            complement(10158..10907)
                     /gene="sufC"
                     /locus_tag="PLF66_22630"
     CDS             complement(10158..10907)
                     /gene="sufC"
                     /locus_tag="PLF66_22630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020768608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HMZ66902.1"
                     /translation="MSELLKIIDLHANIQGKEILKGVNLTIQEGEVHAIMGKNGSGKS
                     TLSNIIMGHPNYEVTKGDILFRGESILSLKTDERARKGIFLCFQYPTGIPGVTVANFL
                     RTVLKSITGKDVSIKEFRKDLKEAMTFLQMDDSFVSRYVNDGFSGGEKKRNEILQMSL
                     LKPKLAILDETDSGLDIDALKIVCDGINKYKSKENGILLITHYQRMLNYITPDFVHVF
                     IDGKIVLTGGKELALKLEENGYDWVTSGAAK"
BASE COUNT         3467 a         1891 c         1820 g         3732 t
ORIGIN      
        1 aatatgagaa gcaatttgcc taaaatcgtg tccactaaat atgtagtagt tcactagata
       61 aactattatt tttaaataca tttactaagt atgatcctga aacaaaaaaa tttaacaatc
      121 cagttcctac tttttatctt tgggctggtg gggctttcta aaactgattg aaagcagggt
      181 aggcgatagt catccagtga ttgaatatca attgatttaa tcgatggagt ccgagaaaga
      241 atacagtgta gtaaatggac tttactctga taaagaaaga gtaaccggac ttaatcctct
      301 aattcaagat ctattaaata aatattttgc tctaacgata acggaaatat ataaataatt
      361 tcaaagttag gtttgtagat tctgtataat cataagactt tttcagaaaa tcggaatagc
      421 atttgttatg atagaccgat aggcgatata attttatgaa ggaaagaagc tattcgtttg
      481 ttctaaattg gaagaagagg cagttttaca agtaaaaatg gttcaaaata atatccgtag
      541 tcatcagaat caatgaatga ctacggataa taccaatgga tatttgctac atgaataaat
      601 ttatttagtg caatagacta atatacttct acttgaggtg ataccaccaa cccagaaatg
      661 aatgatggaa tctacaaaat catgatataa aatgattgca tccacatctt tttcgcaaac
      721 aggggttttg tgtttttcta gttctgcttt tgttgtaaac caagtgcttc taaaagactg
      781 tggtttattt ggagtaaccg gtcttgaata ttcttggaat ccatcaaagt ttgtgatata
      841 acgctttttg tagtctggtt cttctactgg atcaatcact ctcgttggat tgttgacctt
      901 tataattgtt ttcccacatt gtaggaatga aaaaaacgat aaaattaaaa taatatataa
      961 actgtttttc ataaaaccct ccctgtttaa ttgcaagtaa tcttcattgt tcttggcaca
     1021 tagattccaa aagttaactg tgttaaaact gcatcttgcc aacgatagta ttggtctatt
     1081 tcagctattc cagaaggaca gagttgcgtt ccatcaattt ctatttttct aggaaacata
     1141 cctcccaccc aatatttatg gtagagcatt tttacttctc cttttgttcc tcctccctct
     1201 ttttctaatt ttaccctgtt gtggtgacac gctacgagag agaataaaaa taaaataagt
     1261 ataatctttt tcatgctttt agatccttta gtttgaattg ttatttggaa tcgtactaaa
     1321 ttccaaaaat tgttttccat cgaatgccaa tacagattgt cccgttgcac ttacatcaac
     1381 aggaagcgtt gcagaaccga ggcttgcatg tcttgcagta attgtaactg agcctgcggt
     1441 agaagtcgca gtaattgtac actgacctgt tgcactaacg gtcgcgacag tagaattgct
     1501 acttgtccaa gtgatagctg aggatatatc tgtcccattt ttatcaatcg ctcttacaat
     1561 ttttgtatta gtaaatgcta attttacaga tgttaaagca gttgtgctac caatgtcagt
     1621 gattctgact tcaggaattg tagatgttgt ttgacctgga agtgggatcg ggtagattga
     1681 tgtcttttta gaagcgggcg gtggcgggat cagtgcaggc cacgcagtgc aacttataaa
     1741 aagaaataaa atgctaagta taaaaataga ttttgttgat ttcatgttta cctctcttgt
     1801 attaactttc gaatctatat ttttattaca atttactttg ataaatttta attctgttct
     1861 agtgaattca tatttttcat ttattttttt cgaaacacat cttctatttt ctctaagaag
     1921 gtttcctaaa aagcatcttt ctgatggact caggtcgctt ttgtttcctt agaaataaaa
     1981 taagatttct ttagtgcttg taaaatctaa gattgatttt cttttttcaa tttctatttt
     2041 tctgatgttg gaactcgatc tagtttttcc aatgatgaga aattctaaca gttaaatcaa
     2101 ataaagaggt tttatcaatt tgaacttttt gatttttaag aatcccttat tacgaaatac
     2161 aaaagatatt cgatttgaaa tgcaatcctt tttttgaagg ttgaattccc tctcgctcct
     2221 actgaattct taaattagag caaggattta aatataacgt ctttcgaatt ttaacttggg
     2281 agaggactct tttttttgtc tttcgcagat ggttttagtt ttcggaatcg agtgtatttc
     2341 tacattgata aaaacttcaa aaaaggaagc gggaaaatag gtgtttttta gctttttcca
     2401 ttttcaaaaa acctgttttt atagaaatgg aaaaggaatc ctttatgagt ttaccaaaaa
     2461 ataaaaccaa atcttccaga gccttaattg tagaaggggg gggaatgaga ggtgcctttg
     2521 caggtggcgt cctttctgct atgtgtaaat actaccccgt tgataactat gatttagtgg
     2581 tcggagtttc cgctggctct tgctctcttg cttattacac agtggaagag accaatgagt
     2641 tgatttccaa acaaacaatt ttagatattt ggaaacatga gctcaatgga tctaaattta
     2701 tatccttatt aaactatttt aaaggcaaaa aacctttgga tcataaatat ctaatcgatt
     2761 atctgctcgc tcagaaatac agactgaaat ctgagaatct acataaaaac gatacagttc
     2821 cattttatgt agttgtttcg aatttgcata cattacttcc tgaatatgtt cgagctagta
     2881 aagataatgt tctgcagctc cttagggctg ctacatctct tccaattgca actagaggta
     2941 aagaatggct cgatcaaaag ctatattcgg atggtggagt aatggatccc attccaataa
     3001 aagcagtcat cgccgctgga tataaagata tcaccgttgt tttgaacaaa tctagagacc
     3061 atttgtcaga acctgttggg aaattggtaa gttttttgtc ttttccgact ttgcctaaag
     3121 tggcagaatt tttaagggaa aaacatcatt tgatgtacaa tgaagcaaag catataatta
     3181 ataaccctcc aaaaggtata aaaataaaaa tactagaccc aaaggagaat atcgatgttg
     3241 gtttgactac aactaataaa aaatcgttaa tagaaacagt aaaccacggc tgggaagttg
     3301 cctatcgtat gtttaatact acacggcaac gaaccaaatc gtttagaaga aagattgata
     3361 tgtaaatatt ttagattaga taatattgag atgcttatcc agattcgcta ttttgaggat
     3421 agtcattgtt tcatgaccaa tatttttaat cttcaaaata ccatttttct cgtttacata
     3481 tttctgtgaa ttaaaaatgg ctccgatgcc ggatgaatct atatatacat tctcaccaaa
     3541 atctaaaacg atatccaaaa taccattatc gacttgcttc atgattaact cttttaactg
     3601 aatagcattt tttaagctaa tattcccatt taaagagatc gtgcaaactc cattgactac
     3661 ttcgacttct gatttaaaca taattcctct gtgattactc ttactatatc gttcctatct
     3721 tgcaaggaat ttatttgtaa aaaaacattt ttttaaaaat aatttatctt gcaatctatt
     3781 catgaattta tattcattct ctcatgaaaa ttaaattttg gggcgtaagg ggctcaattg
     3841 cctctccaat ttccggtaaa gcaatcaaag cgaaagtaga gaagatttta tccatggcga
     3901 caccttcgga tattcaaagc gaagattcga ttcgaaactt cttggatact ttaaattttt
     3961 ccatattaaa tacttacggt gggaacacta cctgcctcga aattagagac tcaaacaaca
     4021 atctttatat tatagacgga ggcaccggtc ttcgcgagtt gggcaattct atcctaatgg
     4081 aaggtttttt agagggaaaa ggtaaggcaa attggatttt tacgcacacc cattgggatc
     4141 atttgcaagg agttcctttt tttgtgcctt tgtatattcc aggcaatcgt tttgatatct
     4201 atacatcgat tgatggaatc gaagagagaa tacgttatca gcatagaaac acccattttc
     4261 ccgttccgta tgaagctcta tcagctacta aaaattattt ctttgttcaa gaaggatccc
     4321 ctgttaccat taacaatata accctaacct ccaaatcagt tcgtcatccg ggtggaagct
     4381 actcctataa gatggtagag ggggataaga cttttatctt tgcatccgat gctgaattca
     4441 atattgatga aatggagaat atcgatctat atattgaata cttccgtggt gcagatgttt
     4501 tagtgtttga tacacagtat acgtttgatg aatccttaaa gaaaattgat tgggggcata
     4561 gttccgcatc gattgccact gatattgcat tgaaagctgg tgtgaagcaa ctagtaatgt
     4621 ttcatcatga ccctctttac gatgatgata aattggatgc cgtttatcta agggcactca
     4681 attacaaaga aatgtttgat aaaaacaatg aactagacat tatcatggca tatgaaggtc
     4741 tcgaaataga aatctaattt cttgcggata acgcttattt gaagcaaaat tttaaaaatg
     4801 ttttatttag ttcgaatgct atttttacct tctcgaatcg gaagtcgcgg gaatttagaa
     4861 atggattata agggatttcc ttttaaaaat gtcttgacga aattactttg aatgaaacca
     4921 atactataaa aaattatata tccgagtcta gccatcgcag tttttagtaa aaaatctata
     4981 tttattttaa cagtaacagc tgtattgacc ttaattctat caggaagcat tcttttagga
     5041 aaacgctact tcaatcgaac gaaaattgat aagaaattag gtaagtccaa tccgattcat
     5101 tttttagttg tttctgcaaa cgaacaaaag gatattgtgt tctctctttt tgtagctctt
     5161 tatccaacag aaaagaaagt cgccttattt ttcttgaacc cccttattag ttttgatgaa
     5221 caaaaagtag agaagttagg atctgaggca gagaatattc tggttaaaga attagaaaaa
     5281 attacagata gaaaaatcga ttataagctt tctattccag aaaaaaactt ttatagaatt
     5341 attgatgtta tcggggggct gcctgtatat tttgatccat tgcttttgag aaagacttct
     5401 gatagctatg aacgcaaagt aggagaatac aatctctcag gcgaagaact ctctgatttt
     5461 ttaaagctaa aaaatcagga caaccccaca gattatctag aaagactttg gtgtcaagag
     5521 accgttgctc taattctata tgatcaccta agagtgatcc aaaagaatat acgtaaggaa
     5581 tgggttttgc tcttttccac ttttttcgat acagatttat cctctgaaga atttatttcg
     5641 cttcttacat atattcaaga atctcacttg gttttatatg tatcagaaat gccagcagag
     5701 ccaattacga ttacagatgg aaaaaataaa aaagttcaac tcaaagtcaa agaagaagta
     5761 gcaagtatag cctttagtaa atttgtttca gacctacgtt ccgaagattt tgcagatggg
     5821 gaatttgcac gcacggaagt attaaacgga actgaaatca atggacttgc aaaaagcgtt
     5881 aaatcattgt taaatgacaa aagaatcaaa gttctttcta ctgataatgc atggacattg
     5941 acccaagaaa atacaatcat cattgaccgt tctggcaatc ctgaatattc ttataaaatc
     6001 gcagatgttc tcggagtgaa aggaattaaa catattattg acaaagaagt tggtcttgat
     6061 actaccatta ttttaggaga agattttgaa attaaacccg gaaaaaaata aatctgtaaa
     6121 ctcagacaaa gcaagtaaaa atgcgcaaga tacaatcaat acaaaagaga agaaaacttc
     6181 tagacctaaa aaaactacat ccaaaaaaac aaacctagaa tctaaactaa ccgagatacc
     6241 actcaaagaa aaaaaggcaa ctaccaaacc aaaagaaaaa tctttatcta ctgaaacaaa
     6301 aaagatttta aaagaaatta tatctatttt aaaagaaaag aaatgtgaag atattattgt
     6361 gatgaatttg gaagaggtaa atacctattt gtctctattt gtaatttgca cggtggcgag
     6421 tcatacacag ggtagagcag cagctagaga tcttgaaaag aaaatgaagc attacaagtt
     6481 aggagtaggg aatacagaaa agaaaaacat acctactgaa tctggttgga ctctgcttga
     6541 tctaggagaa attgtagttc atatcatgac agcagaaaca agagcgtatt atgatctaga
     6601 taaactttgg ggagatgcaa agcggattac gatttaattt tagtcgtaaa tccctagctg
     6661 catttttgca tcttctgatg ccatgattct tggatcccat ttcggattcc aaaccacctc
     6721 tacatttaca tctttaattc catcgacttg tgctgctgcc gaaagaacct gtgatttcat
     6781 agaaggacca gcaggacaag ccatgctagt aaaagtcatt ttgatatttg caatattttc
     6841 ctcatttact tttatatcgt aaacgagtcc tagatccatg atcgagataa ataattctgg
     6901 atcttcaatt ggtaaaatag cttggtaaat tgctttttct aagtcatttc ttagttcttc
     6961 catttttatc cctttaatgc tttatctaac gtattccaac aaagaaccgc acattttact
     7021 cggacaggat attttttaac tccatttaga gcttctaaat ctgcttcttc atccgaataa
     7081 tttggatctt tatcttctaa tatcattcct ttgaattttt caatcagtgt aaaagcttct
     7141 tcttttgtct ttccttgtat gagttctgtc atcatagatc ccgaggcttg cgaaatagaa
     7201 cacccttttc cttgaaagct tgcttcctta attttgtctc catcaaaaga taaaaagagt
     7261 tcaaattcat ctccgcagag aggattcact cctctctctc ggacatttgg atttgctaaa
     7321 acgtaataat ttctgggatt ttcaaagtga tctaaaatca cttctttata tagttcatcg
     7381 cttaacgaca cgggcaaata tctccataac tttttttaat ccttctacta atgcatctac
     7441 atcttcttta gtgttgtata gataaaagct agctctacag gttcctgcta ttcccatctt
     7501 tttcatatat ggttggcagc aatgatgccc tacacgaatc gcaatccctt gctcatctag
     7561 aatcgatcct acatcatgcg catggactcc ttctacatta aaggatacta ctccacctct
     7621 gtgattcata tccttcgttc catagagtgt aattccacct aggtcttcga ggcgaacgat
     7681 cgcatacttt actaattcca cttcgtggtt gtgtatattt tccattccaa cagaatttaa
     7741 gtaatccatt gcgtgagaaa aagcgatccc acctgctata tgaggagtgc ctgcttccaa
     7801 tcttgccgga aggtctgcat aggttgtcca ttcttttgtg acctcagaga tcatgtctcc
     7861 accacccatc caaggaggca ttctttctaa gatttcctct tttccatata gaattcccac
     7921 accagtagga ccaagcatct tatgagcaga gaaaacatag aagtctacat ctaattcttt
     7981 aacatttgtt ttgatatgag aagcaccttg tgctccatct agtaaaaaca aagctccaac
     8041 cgaatgagcc ttttcgatta acggtttaat ttcatgaata gttcctgttg catttgacat
     8101 ctgtgaacat acgactaatt tagttttata tgtgatgata gagtcaatat cacttaagtc
     8161 taacgttgaa tcttcattta gcggaatgta ttttaaagat gctccttttt caagtgctag
     8221 catttgccaa ggaacaatat tggagtgatg ttctaattca tttagaacta tttcatcacc
     8281 gcgattaata tttgctcttc cccaagtctg ggcaactaag ttgatactct ctgttgcatt
     8341 tcgtgtataa attgcgattt tagcgcaacg agcgccgatg aagtctgcta ccttgattcg
     8401 agtcttttca tataattcag ttgcatgctg ggaaagataa taaacccctc tatgaatgtt
     8461 tgcattttct tctttgtagt atttatcaat tgcgtcaatg acctgaaagg gtttctgaga
     8521 actggcagca ctatccaaaa atacaagagg ctttccattc attgttgtat ttagaatggg
     8581 gaaatctgtt tttaatctat aagggtttag gctcatgtta atcctcaatg tctacttcaa
     8641 tcatattgtc tgaaattcga acaggaaatg ttaccaaatc ttctgttgct ggcatacaca
     8701 aagctttgcc attttttaaa tcaaattttg caaaatgtct tggacagata atggctccgg
     8761 attcaatttt tccagatgaa atttcttccc catcatgagt gcagacatcc tcaaatgcga
     8821 taaaatcctc tcccaacttt gtaatcgcaa tgtttgcata ctttgtcttt actactttaa
     8881 ttagaccttc ttttacttct gcaacattta ttaatttttt atacataaga tcccttttaa
     8941 ttgtatagct ttttagttaa acgactgact agataagatt gaatttcttc tttgtctact
     9001 aaatctaaaa tttcttttaa aaacccttca atgattaaaa atctagattc attttccgta
     9061 agtcctctag acatgagata aaaaagttgg tcttcgttca cttccccaac ggttgctcca
     9121 tgggaacatt ttacatcatc tgcgaatact tctagcttgg gcatagattc cgctctagca
     9181 gttctagcca tagtaagatt attattgatt tggcttgctg tcacatgaag agaagtcttt
     9241 ggtataaaca gatttcctgt aaatacatga tgcgatttat cttttacaac tgcttttagc
     9301 tttaactcac tatctgttct gctactgaag tgggaaatat taaactctat atcctgaaat
     9361 tctttttttg cgagtgttgt tgccccatga gaaattaaat ttgctccctc accagaaaga
     9421 tcgtggttaa tgagtgtttt ccccttgtag ccacccaagt aaaaatgaaa tagatttaga
     9481 gctgaagctc tgccctgatt agattcaata tttctaaaat gaaatgtaga agattcgaag
     9541 tcttcaatgg ttataaattt agtttgtgaa ttttctgata gttctatata actaaatgga
     9601 ttgattaagt taaaatcttc tgtaatagga gagttgaatt tttctacaat ttttacactt
     9661 gtattcgatt ctattttcca aaataaaaga ggttgaaacg atgtttctcc ttgtggatat
     9721 tgaacatcca tttcgatata ggattctatt attttattct ttggaacctt aattaaaatc
     9781 ccaatatcaa aaaaactgaa attcaaaaga gcaaagtaat ttgctttgta tcgatctaat
     9841 aatttttgaa agctatcttg gataaactga atatccattt cctctagctt tgaaaaatca
     9901 ttggaatgaa tctcgtccat attgattgag aagctagaag attttttatt ggctgataga
     9961 agtgtctcta ttgagaaatt agaaagattg atctttctcc aagattcttg tttcgaattt
    10021 ggcaatggag tattttctaa aatggcaaat gctttttttc tgagctgaat tagccaatct
    10081 ggctctttcc tattggatag aaaggaatca aactgagaag atagaaatgt tttattagca
    10141 gtagctactt ccatacttta ttttgctgct ccactagtta cccaatcata accattttct
    10201 tccagtttta aggctagttc ttttccgcct gttagaacaa tttttccatc aatgaataca
    10261 tgaacaaaat caggagtgat atagtttagc attctttggt agtgtgtgat gagtaagatt
    10321 ccattttctt ttgatttata tttattgatt ccatcgcaaa caattttcaa tgcatcaata
    10381 tctaatccag aatctgtttc atctaggatt gctaatttgg gttttagaag agacatttgt
    10441 agaatttcat ttctcttttt ttcgccacct gagaatccat catttacata tctacttaca
    10501 aacgaatcat ccatttgtag gaaggtcata gcttctttta gatcttttct aaattcttta
    10561 atcgatacgt ctttaccagt tatacttttt aatacagttc ttaaaaagtt tgcaaccgta
    10621 acacccggta tacctgttgg atattgaaaa caaagaaaaa ttccctttct tgccctttca
    10681 tccgtcttta gagaaagaat tgattctcct ctaaaaagaa tatcaccttt cgtaacttcg
    10741 taattaggat gacccataat gatattggaa agagtgctct ttccagaacc attttttccc
    10801 atgatagcgt gaacttcacc ttcttggatc gttaggttaa caccttttaa aatttccttt
    10861 ccttggatgt ttgcatgtaa atcaatgatc ttgagtaatt ccgacaaaat
//
LOCUS       DAOQOS010000154        10869 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_3134_length_10869_cov_5.786480, whole genome shotgun sequence.
ACCESSION   DAOQOS010000154 DAOQOS010000000
VERSION     DAOQOS010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 10869)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10869)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10869
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3134_length_10869_cov_5.786480"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1208
                     /locus_tag="PLF66_22635"
     CDS             <1..1208
                     /locus_tag="PLF66_22635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66903.1"
                     /translation="VSFWKPKIVSFFYVGVQSLANIDSKLGSALAVNVGNFPSTQNVS
                     ASSLPLPTGAATSSNQATTNTNLGAQGDSAATNDTGSWSLIALFKRLLQSITNLLNNS
                     PLGQADPIASRPVVICNEQQINGSSLSAVNTNLLTGNVNDWYDARGFNSACIQIIGSA
                     GIASGQIIFEQTNDNVNAVAGRPLPAYEVDVQNANPIIAAFNIAANSIRMFNVAVNAR
                     YIRVRISTVFAGGTVQAVGVLSQLPFAYPTVNIQQATAANLNANVSGTVTVNSLPAGT
                     NAIGDVGTQYRANATGAASKYHLIAAASVNNTNLKATAGRLLGWQLANTTASWLYVKL
                     HNQSTAPTAGASVFMTIAIPPNGVNCLHFEGGIAFSTGIGITTVTGAADSDTTAVTAN
                     SIVGTLVYM"
     gene            1272..2354
                     /locus_tag="PLF66_22640"
     CDS             1272..2354
                     /locus_tag="PLF66_22640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004473422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66904.1"
                     /translation="MFYNNLTRTWDRNTAFDGLFFAAEFARLLGNDIELKSMIDTFIA
                     NTFKSDKKELGEVFFLNELKSPSADFPAICLAQNNAKIWKNSNPLGAGNIPGNYPDLV
                     DYLNAIPLRFDPLNGNNIDFTVTDWSYSGGVITLTFAYTTPNGAKESKVIRSLHEDAF
                     VHNSNESIGVDYSAFEAVTSWRTITLVDPLRDSSDVVQVPAGTYQITAIAGGESAAAQ
                     IKFVHTATPSGTPKTESRLVRFYPHRIQDAGQNLDIQASIKAQHFAVQGRGFVTVMDG
                     DSEWVGGLRRRDRFQKFSMKINASEYYVLSAGSGVASGVGSYGVGSAVDVNTFYANGG
                     GSPRNSKTTDARGYALYPYIWAKSYV"
     gene            2586..3200
                     /locus_tag="PLF66_22645"
     CDS             2586..3200
                     /locus_tag="PLF66_22645"
                     /inference="COORDINATES: protein motif:HMM:TIGR01065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HMZ66905.1"
                     /translation="MYKGEKINSISHLVGASLSLAGWVLLIVFSSLTGDPWKIVGCTI
                     YGFSLFFMYLFSTLYHSFKGKAKRVFQIFDHIAIYILIAGTYTPITLVTLRGKMGWNI
                     FGLVWGIAVVGTIFKTAWTGKFDRISTAFYVLAGWTILIDISELYEKFPRDGFYWIFA
                     GGVLYTIGAFFYLRDSLKRNHEIWHFFVLGASICHYVAIFFYLI"
     gene            3267..3575
                     /locus_tag="PLF66_22650"
     CDS             3267..3575
                     /locus_tag="PLF66_22650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66906.1"
                     /translation="MKILILLIAIVLFFKCDVGFGTSGRDHCKNYAPITLDGPVKTSK
                     ANQFRQCMNFLNAFNQSRNENKESGSLDYYLIQCSIKYKKLKECDNESPLPYPIPIKY
                     "
     gene            complement(3702..4553)
                     /locus_tag="PLF66_22655"
     CDS             complement(3702..4553)
                     /locus_tag="PLF66_22655"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HMZ66907.1"
                     /translation="MKYEYLETEDGFNIGYSVVGEGTPILVIGSKDYYPKTFPNLPKK
                     DFQLIHIDTRAFCNPEKEFPESAFELSKFLNDIQKIQNRLNIPKAVILGHSIHSYFAL
                     EYSRQFPNLTSGLILLATSPFSGFQLIETANRYFGEIADEKRKSLLNQNLQTLEMETQ
                     KNPEKAFVIRMLKFAPMIWYDPSFDANDLWEGVQLNSQGANLVWGKMFSEYKVKEILP
                     SISVPIFLGLGKYDFWNPVLLWDEFKNISNLKIKIFEKSGHTPQLEESELFYKELKNF
                     LIENHLY"
     gene            4812..5204
                     /locus_tag="PLF66_22660"
     CDS             4812..5204
                     /locus_tag="PLF66_22660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020776602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HMZ66908.1"
                     /translation="MNLTKNKEIVTEFFTNLNEQKMKEAFEMLSEDLHWWILGNIPVS
                     GDYDKRKISLGLKMLQRSFDGFKFEIGVMTAEEDRVSVIVESHAKRKSNGKTYNNHYH
                     FLISIKDDKIYSVREYFDTVHAVWVEED"
     gene            5252..6379
                     /locus_tag="PLF66_22665"
     CDS             5252..6379
                     /locus_tag="PLF66_22665"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HMZ66909.1"
                     /translation="MDNTKYASFTERFLAGYFDAVLFLFLFLMIPYELLTCYNKVWGI
                     VLILCYSGLMKISYSVLLNYKFGQTFGKKLFNIKVVKDDLSPLNIRNALLREIIWILL
                     LSLVFYFLYIPSQLMSIKEIHNISPFDFEYSLGYYWNGKERILNFIPLPIILTDAFSF
                     FIHKDKKSIHDWIGGTIVIRTKEPNAYIKVIHYLLMPLVLSVIIILHRAPAKQNYSTI
                     YKHEIYTAIDQLNANLLVEILENEPELVNKKDPMGKTLLHYLSEVKYRTENSDSSEKI
                     PLAEILLAFSLEINEKDIAGNSALHSAIATTLTWDCPYYEFLIKNGANVDLKNREGLL
                     PTHAFYSKLNLLERYMLNGNHFDTLESCAREKEISTEVKEK"
     gene            complement(6452..6805)
                     /locus_tag="PLF66_22670"
     CDS             complement(6452..6805)
                     /locus_tag="PLF66_22670"
                     /inference="COORDINATES: protein motif:HMM:NF037832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HMZ66910.1"
                     /translation="MKFKIDENLPIEIAELLREKNFQADTVNEENLSGVKDSIISDTC
                     KKEDRILVTLDLDFSDIRNYPPIKYPGFIVLRLKSQDKESIINVFKKFIPFIQEENIQ
                     NKLCILEENKVRIRE"
     gene            complement(6802..7023)
                     /locus_tag="PLF66_22675"
     CDS             complement(6802..7023)
                     /locus_tag="PLF66_22675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002771983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HMZ66911.1"
                     /translation="MNTADFISYDPKICHGKPCIKGTRIMISVILDNLAAKEPIASIL
                     KSYPSLKEEHIYASIQYAADLARERVVLI"
     gene            complement(7092..10196)
                     /locus_tag="PLF66_22680"
     CDS             complement(7092..10196)
                     /locus_tag="PLF66_22680"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HMZ66912.1"
                     /translation="MKHVFLFLFLILSLYSNPTDKKFISTNYTSTDGLANNIVSSITQ
                     DSQGFLWIGTYGGLNRYDGKEFKTYTMTNGLAFDAVRAVYADVKEKQIENDIWVGTEL
                     GLSILRNGKVLEDSKSKPFLDFFKGQNIRAILKTKDDIYLVGTVENLFTIGKDNKVEA
                     VKSAKGKQIQTLFLASNNTIWVGTKREGLFQLSKDSKSIQKVGSIYSEILSIQEEISS
                     KEDSSKKLIVSYADDRIYSYTLEGEEEEIYNPQIKSISKKGRYLICKNNLNQTLSFFS
                     KIEDSYLYEDNSYSEYSIGNINTCYIDREGTFWVGTYGSGLTKFYRRKVDSYTNINGL
                     PNPNVRYIIKNREGDLWIGTQFSIFSKVNEKFKVIDENPKKKKALDKVRAIIEDDDNR
                     IWFGSENGLLYLEGKKLKEIQLEKSKNANIYSMDHFNGFLYILEGKGKVYRIDLNDTS
                     QVNVITFKPEEEVPWRIIRSLDKKNLYLQTSKRILELKKKSKKELEEEAAKANETPAK
                     ETNPSSDKKILEADEKFTQLIRKDEIKASNGEEIEVLNKIQSFLPIEKNHFVFGDNKL
                     FIKKGKEIRVKTQKDGLPEGQIVSISKDDENNLWIGTSKGIANYKQATGEIISFNHND
                     GLAGDFCNLNSIATDDEYAYFGTSEGLSRIEHTHKIKNGIEPNVYFTLIKTNIDSIGK
                     DQFQNLDFDYSVNTVTLDAATLSLVAPENNKYEFIHNFNGKEIARNIQNSGTITYYNL
                     EHGTHTFIVKGKNSDRVKSYNEDKIEIHIAAPFWLDLRYMIPLTLLAFGLIYGIYALR
                     VYQIKQENIKLEKLVQQRTKELFEEKETSERLLLNILPKNIAERLKNGESTIADSFVE
                     VTVLFADIVGFTKLSQTVTPSELVARLNDLFSRFDKISIEMNVEKIKTIGDCYMAVAG
                     LPEVRKDHAFLMIEMARKMIAAIDDFNQIHHTTLSMRIGMNTGEVVAGVIGTHKFIYD
                     LWGDAVNTASRMESHGVPGKIHVTESTYRLLKDKYDFESRGTLEIKGKGQMNTYLLTD
                     IKHG"
     gene            complement(10239..10715)
                     /locus_tag="PLF66_22685"
     CDS             complement(10239..10715)
                     /locus_tag="PLF66_22685"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HMZ66913.1"
                     /translation="MKKNIFPIVSILLLIIYAGYSYQKYRIAPGFSFSNLELINEKKE
                     TSFPIQKDGKWKVISFYASWCIDCKREFPNMKKAMETNLSDFSFIAITDEGYEKMISY
                     KYTNEYPFQFFSLLKDFENYQIYAIPTTYILNPENEIVYGKIGSVDWQSIRSLMKN"
BASE COUNT         3255 a         1787 c         1939 g         3888 t
ORIGIN      
        1 tagtctcctt ttggaagcct aaaatcgtgt ccttttttta tgtaggggtt caaagtcttg
       61 ccaatattga ttcaaaactt ggctctgcat tggctgtgaa tgttggcaat ttcccatcta
      121 ctcaaaatgt gagtgcaagc agtttgcctc tgccaaccgg agccgcaacg agttcaaatc
      181 aggcaaccac taatacaaat ttaggagcac aaggagattc agcggcaaca aatgatactg
      241 gatcttggag cttgattgct ttattcaaac gtctcttgca atcgattact aatttgctca
      301 ataattcccc tcttggtcag gctgatccta tcgcatctag acctgtcgtt atttgcaatg
      361 aacaacaaat taatggatcc tccttatctg cggttaatac taatttgctc actggaaatg
      421 ttaacgattg gtatgatgcc agaggtttta actctgcttg tatccagatc attggaagtg
      481 ctgggattgc atcggggcaa attatttttg aacagacgaa tgataatgtc aatgctgtgg
      541 ctggtcgtcc tttacctgca tacgaagtag atgttcaaaa tgcgaatcct attattgccg
      601 cttttaatat tgcggctaat agtattcgca tgttcaatgt tgcggtaaat gctcgttata
      661 ttcgtgtgag aatttcgacc gtgtttgctg gtgggactgt gcaagctgtg ggggttttat
      721 ctcagttgcc ttttgcctat ccaactgtaa atattcagca agcaactgcg gctaatctaa
      781 atgctaatgt gagtggaacg gtcactgtaa actcattgcc tgctgggacc aatgcgattg
      841 gggatgttgg aactcaatac agagcgaacg caaccggagc ggcttctaag tatcacttaa
      901 ttgctgctgc ctctgtaaat aatacaaatt tgaaagcaac tgcaggtaga ttgttaggtt
      961 ggcaattggc aaatactaca gcctcttggt tgtatgttaa gttgcataat caatcgactg
     1021 ctcctactgc gggggcaagt gtatttatga caattgcaat tcctcctaac ggtgtaaatt
     1081 gcctacactt tgaaggcggt attgcgttct ctacagggat tggtattact actgtaactg
     1141 gcgctgctga tagtgataca actgccgtta ctgcaaattc gattgtaggg actttggtat
     1201 atatgtaaga gcggtttgat acgtaaagta ggcgcggtta cgtaaccgcg ctttcacaga
     1261 aaggagaaga tatgttttat aataatttaa caagaacttg ggatcggaat actgcgtttg
     1321 acggtttgtt ttttgctgct gagtttgctc ggcttttagg gaatgacatt gaactcaaaa
     1381 gcatgattga taccttcatt gcaaatactt tcaagagtga taaaaaagaa cttggagaag
     1441 tgtttttctt aaatgagttg aaatctccaa gtgcggattt ccctgcaatt tgtttggctc
     1501 agaacaacgc aaagatttgg aagaattcaa atcctttggg ggctggaaat attcctggta
     1561 actatcctga tttagttgac tatttgaatg caattcctct tcgatttgat ccgcttaacg
     1621 gtaacaatat tgactttact gtcaccgatt ggagctattc aggaggtgtg attactctga
     1681 catttgccta tacaactcca aatggagcca aagaaagtaa agttatccga tcgcttcatg
     1741 aagatgcgtt tgtgcataat tctaatgaat cgattggagt agattattca gcttttgaag
     1801 ctgtcacttc ttggagaacg attactctag tcgatccact cagagactct tctgatgtag
     1861 ttcaagtgcc tgctggcacc tatcagatta cggctatcgc tggcggtgaa agtgctgctg
     1921 ctcaaattaa atttgttcac acagccacac cctctggcac tcctaaaact gaatctcgtt
     1981 tggtgcgttt ttatcctcat cgcattcaag atgcagggca aaatttagat atccaagcat
     2041 cgattaaagc tcaacatttt gccgtccaag gtcgtggttt tgtgactgtg atggatggtg
     2101 attctgaatg ggttggggga ttgaggagac gggatcgatt tcagaaattc tcaatgaaga
     2161 ttaatgcgtc tgaatactat gttctgtctg ctggctctgg ggttgcgtct ggtgttggat
     2221 cgtatggtgt tggctctgca gttgatgtca ataccttcta tgcaaatggg ggtggctctc
     2281 ctcgaaacag taagactact gatgcgcgtg gatatgcgtt gtatccttat atttgggcga
     2341 agagttatgt gtaagttttt tgcaaaaaag ttttctagtt aacactttag tgcgcatttt
     2401 gattttcagt tatgcgcaca aatagcattt gattttttct ttctaaaaat ttcaatttcg
     2461 ctcaaaaagg ataaaggcca actagagtga tgccatttct ttgttttcaa tagagaaaat
     2521 taaatcactt gaccttttta attcagactc aatagtcaaa aacaggtttt ttataggaga
     2581 caattatgta taaaggtgaa aaaatcaatt ccatttctca tttagttgga gcgagtttaa
     2641 gccttgcggg ctgggtgctt ctcattgtat tttctagttt gacgggtgat ccttggaaaa
     2701 ttgtgggttg cacgatttat gggttctccc ttttttttat gtacctattt tcgaccttgt
     2761 atcatagttt taaaggaaaa gcaaaaagag tatttcagat ctttgatcat attgcaatct
     2821 atattctaat agcgggaacc tataccccga ttactcttgt gacgttacgc ggcaagatgg
     2881 gttggaacat ttttggactt gtttggggaa ttgcagtagt tggaacgatt tttaaaactg
     2941 cttggactgg aaaatttgac cgaattagta cagcatttta tgtgttagcc ggttggacta
     3001 ttttgataga tatttctgag ctttatgaaa agttcccacg ggatggattt tattggattt
     3061 ttgctggtgg ggttttatat acgataggcg cattctttta tttgagagat agcttaaaac
     3121 gaaatcatga aatctggcat ttttttgttt taggggcaag tatttgtcat tatgttgcga
     3181 ttttctttta tttgatttag gtttttttaa aagaaagaaa atatttatct taggattggc
     3241 tactgttgaa tagccgttaa aagcaaatga agatattaat actattaata gctattgttc
     3301 ttttttttaa atgcgatgtg ggttttggaa catcgggtag agatcactgt aaaaattatg
     3361 cacctatcac actagatgga cccgttaaaa ctagcaaggc aaatcagttc cggcaatgta
     3421 tgaattttct gaatgcattc aatcaaagta gaaacgagaa taaagagagt ggttcactcg
     3481 attattattt gattcaatgc tctataaaat ataaaaagtt aaaggagtgt gacaatgaaa
     3541 gtcctcttcc ttatccaata cccattaaat attgaactta gtaaaatatt tgaatcaaaa
     3601 aaatcgaaac agagttagta actgctatct ttcgcaaaag gggtattccc aattcccaaa
     3661 agacagtttt ctagtttcta atcggaattt acttttggga attagtataa atgattttcg
     3721 attaaaaagt tttttaactc tttgtagaaa agttcagatt cttccaattg aggagtgtga
     3781 ccgcttttct caaatatttt aatttttaaa tttgaaatat ttttaaattc atcccataga
     3841 agaacaggat tccaaaaatc atatttacct agtcctaaaa aaattggcac tgagatagaa
     3901 ggtagaattt ctttgacttt gtattcggaa aacattttac cccaaactag atttgcacct
     3961 tgggagttga gttgaactcc ctcccataga tcatttgcat caaagctagg atcataccaa
     4021 atcataggag caaattttaa cattcgaatc acaaatgctt tctctggatt cttttgggtt
     4081 tccatttcta atgtttgcaa attttgattt aatagacttt ttcttttttc atctgcaatt
     4141 tctccaaagt atcgatttgc agtttcaatt aattgaaatc cagaaaaggg acttgtggct
     4201 aataatatta atccgcttgt taaattaggg aattgtctag aatattccag cgcaaagtaa
     4261 gaatgtatgg aatgccccaa aatgactgcc ttgggtatgt ttaatctatt ttgaattttt
     4321 tgaatatcat ttagaaattt ggaaagttca aatgctgatt caggaaattc tttctctgga
     4381 ttgcaaaagg ctctggtatc aatgtgaatc aattgaaaat cttttttagg tagatttgga
     4441 aaagtttttg gataataatc cttacttcca attactagga taggagttcc ttctccaaca
     4501 accgaataac caatgttaaa tccatcttcc gtttctaaat attcgtattt catttttact
     4561 ccttttttaa aacccaatat ttaaatcaga tttagtcaac aggaaaacac tatattttgt
     4621 ttattgatgg atttagaaaa agtgaggaat ttaattttac aagatatcta gactccgaaa
     4681 atctacctag aaaataaatg aattgtctag tatagtctat aatgtgaatc gaattcgatg
     4741 gaaaaaaagt aggcattaac cgctagaaaa aattaaatat caaaatctag aataaaaaat
     4801 gggaaaataa aatgaatcta actaaaaaca aagagattgt aacagaattt tttacaaatc
     4861 taaatgaaca aaaaatgaaa gaggcgttcg agatgctttc agaagattta cattggtgga
     4921 tcttaggaaa tatcccagtc tctggcgatt atgataaaag aaaaatcagt ttaggcttga
     4981 aaatgcttca gcgcagtttt gatggattta aatttgaaat tggtgttatg acagcagaag
     5041 aagatcgagt cagtgtcatt gtagaatctc acgcaaagcg aaaatcaaat ggaaaaactt
     5101 ataataatca ttatcatttt ctaatttcca tcaaagacga taagatctat tcagtgagag
     5161 agtattttga cacagttcat gcggtttggg tagaggaaga ttgacggata ctttttatta
     5221 gccgttagaa agaaattaga aaaagtaaat tatggataat actaaatatg caagctttac
     5281 agaaagattt ttagctggtt actttgatgc tgttcttttt ttatttctat ttttaatgat
     5341 tccatatgaa ctcttgactt gttataataa agtttggggt attgtgctca ttctatgtta
     5401 tagtgggcta atgaagatta gctactctgt tttattaaat tataaatttg gtcaaacttt
     5461 tggaaaaaag ctatttaata taaaagtagt aaaggatgat ttaagtcctt taaatattag
     5521 gaacgcatta ctaagagaga ttatctggat tttactttta agtttagtat tttattttct
     5581 atacatccca tcgcaattaa tgtctataaa agaaattcat aatatatcac catttgattt
     5641 tgaatactca ctaggttatt attggaatgg taaagaaaga atactaaatt ttattccttt
     5701 gccgattata ctcacagatg catttagttt ttttatacac aaagataaaa aaagtattca
     5761 tgattggatt ggtggcacaa ttgtgattcg aacgaaagaa ccaaatgctt atataaaagt
     5821 aattcactat ttactaatgc ctttagtttt atctgtaatt attattttac atagagcgcc
     5881 agctaagcag aactattcca ccatttataa acatgaaatt tatacagcta tagatcagtt
     5941 gaatgcgaat ttgcttgtgg aaattttaga aaatgaacca gagcttgtaa ataaaaaaga
     6001 tcctatggga aaaacattgc tacactacct atcagaggta aaatatagaa cagaaaacag
     6061 tgattcatct gagaaaatcc ctttagcaga aattctatta gctttttcgc tagaaataaa
     6121 tgaaaaagat attgctggaa actctgcctt gcattctgct attgcaacta cattgacatg
     6181 ggattgtcct tattatgagt tccttataaa aaatggggct aatgttgatt tgaagaatcg
     6241 cgaaggatta cttccaaccc atgcctttta ttcaaagctc aatttgttag aacgttatat
     6301 gctaaatggt aatcattttg atactttaga atcatgtgcg cgagaaaaag aaatatccac
     6361 agaagtcaag gaaaagtaat aattaaatag agagggtcct ctttattggc cgcttgcatg
     6421 ctactcttag ttaagaatgt aaacgttatg ctcactctcg aattctaact ttattttctt
     6481 ctaggataca gagtttattt tgtatattct cttcttgaat gaatggaata aattttttaa
     6541 atacattaat aatactttct ttatcttgtg attttagcct taacactata aaccctggat
     6601 atttaattgg tggataatta cgaatatccg aaaaatctaa atctagagta actaaaattc
     6661 tatcttcctt cttgcaagtg tctgaaatta tagaatcttt tactccagat aaattctcct
     6721 catttactgt atcagcttga aagtttttct ctctaagtaa ttctgcaatt tcgataggaa
     6781 ggttttcatc tattttaaat ttcaaattaa aacaactctt tctctcgcta aatcagctgc
     6841 atattgaatg gatgcgtaaa tatgttcttc ctttaacgaa ggatagcttt ttaaaatact
     6901 tgctattggc tcctttgctg caaggttatc taggatcact gagatcataa ttcttgttcc
     6961 ttttatgcaa ggtttaccgt gacaaatctt tggatcgtat gaaataaaat cagcagtgtt
     7021 catgattcca ttttctactt gttcttttta attttcaact tatttttctc aacaaaatga
     7081 tacagttacc atcacccatg tttgatatca gtgagtagat aggtgttcat ttgtcctttg
     7141 cctttgattt ctaaagttcc cctagattca aaatcgtatt tatcttttaa gagtcgatag
     7201 gtgctttcgg tgacatgaat ttttcctggc actccatggc tttccattct acttgcagta
     7261 ttgacagcat ctccccaaag atcataaata aatttatgag ttccaatgac tccggcaact
     7321 acttctcctg tattcattcc aattcgcata gaaagagttg tgtgatggat ttgattaaaa
     7381 tcatctattg cagcaatcat ctttctagcc atttcgatca tgagaaaggc atgatctttt
     7441 cttacttctg gaagaccggc aaccgccata taacaatctc caattgtctt gattttttcg
     7501 acgttcatct caatcgaaat tttatcaaag cgactgaata gatcattgag tcttgcgact
     7561 aactcacttg gtgtaactgt ttgcgaaagt ttagtaaatc caacaatatc tgcaaaaaga
     7621 accgtcactt ccacaaagga atcggcgatt gtggactcac cattttttaa tctctcagca
     7681 atattttttg ggaggatatt taaaagtaac cgctctgatg tttctttttc ttcgaagagt
     7741 tctttggttc tttgttgcac caatttctct agtttgatat tttcttgttt gatttggtaa
     7801 actctaagtg catagattcc atatataagt ccaaatgcta agagtgtgag tggaatcata
     7861 taacgtaaat ctaaccaaaa gggagctgca atatgaattt caattttatc ttcattataa
     7921 cttttcacgc gatcactgtt ttttcctttt acaataaaag tatgagttcc atgttcaagg
     7981 ttgtagtagg tgattgttcc tgaattttga atatttctgg caatttcctt tccattgaaa
     8041 ttgtgaatga attcatactt attattttca ggagctacaa gacttaaagt agctgcatcg
     8101 agtgttacag tatttacact ataatcgaaa tccaaatttt ggaattgatc ttttccaatt
     8161 gagtcaatat ttgttttgat tagagtaaaa taaacgttag gctcaattcc attttttatt
     8221 ttgtgcgtat gctcaattct agacaagcct tcactcgttc cgaaataagc atattcgtca
     8281 tctgtcgcaa tcgaatttaa attacaaaaa tctcctgcaa gcccatcatt atgattgaag
     8341 gatataattt cacctgtcgc ttgtttataa ttggcaatac ctttgctagt tccaatccaa
     8401 agattgtttt catcatcctt ggagatactg actatttgcc cttcagggag tccatctttt
     8461 tgagttttga ctctaatttc ctttcctttt ttaataaaga gtttgttgtc tccaaataca
     8521 aaatggtttt tttcaattgg taaaaaggat tggattttat ttaaaacttc gatctcttct
     8581 ccattggatg ctttaatttc atctttacgg atgagctgtg taaacttctc atctgcttct
     8641 aatatctttt tatcggaaga ggggtttgtt tcttttgcag gtgtttcatt tgcttttgct
     8701 gcttcttcct ctaattcttt tttagatttc tttttgagtt ctaaaattct cttagaagtt
     8761 tgtagatata aatttttctt gtctagactt cgaatgattc tccagggaac ttcttcttct
     8821 ggtttaaatg taattacatt tacttgtgat gtatcattta gatcgatgcg atagactttt
     8881 ccctttcctt ctaaaatata aagaaatcca ttaaaatgat ccatagaata aatatttgca
     8941 tttttacttt tttctagttg gatttccttt aatttttttc cctctagata gagaagacca
     9001 ttctctgatc caaaccaaat tcgattgtca tcgtcttcta taatcgctct tactttatcg
     9061 agtgcttttt tcttcttcgg gttttcatcg attactttaa atttttcatt tactttgcta
     9121 aaaatactga attgggttcc aatccaaaga tctccttccc gattttttat aatatagcgc
     9181 acatttggat ttggtagacc gttgatattc gtataactgt ctacttttct gcggtaaaac
     9241 tttgttagcc ccgatccata tgttccaacc caaaaagttc cctctcgatc aatataacag
     9301 gtgtttatat ttccaatgga atactcgcta taagaattgt cttcatatag ataggagtct
     9361 tctattttag aaaaaaatga taaggtctgg tttagattgt ttttgcagat cagataacgt
     9421 ccttttttgg agatactttt gatttgagga ttatagattt cttcttcctc tccttctaaa
     9481 gtgtaactat agattcgatc atcagcatag gatacgatta gttttttaga ggaatcttcc
     9541 ttggaggaaa tttcttcttg aatcgataaa atctctgaat agatacttcc gaccttttgt
     9601 atgcttttgg agtctttact caactgaaag agtccttctc ttttggttcc tacccagatg
     9661 gtattattgc ttgctagaaa aagagtttgg atttgttttc ccttggctga tttaactgct
     9721 tctactttat tgtcttttcc aatcgtaaat agattttcaa cagttcctac taaatagatg
     9781 tcatcttttg tttttagaat cgctcgaatg ttttgtcctt taaaaaaatc gagaaagggt
     9841 ttacttttac tatcctctaa aacttttccg tttcttaaaa tgcttaagcc taattcggtt
     9901 cctacccaaa tatcattttc gatttgtttt tcttttacat ctgcatagac agctctcact
     9961 gcatcaaaag ctaaaccgtt agtcatggta taggttttaa attctttacc atcatagcga
    10021 ttgagacctc catacgttcc aatccataaa aagccttggc tatcctgtgt gatggaactc
    10081 acaatattat ttgcgagacc atctgtagat gtgtaattag tggagataaa ttttttatca
    10141 gtaggattag aatataagga gagaattaga aaaaggaata ggaacacatg tttcattgtt
    10201 ccagttcaat ttaaggttag ttttagcaaa gcaaaaattc agtttttcat tagagacctg
    10261 atactctgcc agtctacact tcctattttt ccataaacga tttcattctc gggatttaaa
    10321 atataagttg ttggaattgc atagatttga tagttttcaa aatctttcag gagagagaaa
    10381 aattgaaaag gatattcgtt tgtgtatttg tatgaaatca ttttttcgta tccttcatcg
    10441 gtaattgcga taaacgaaaa gtcagataga tttgtttcca ttgctttttt catgtttgga
    10501 aattctctct tacaatcgat acaccagctt gcgtaaaaag agattacttt ccattttcca
    10561 tctttttgaa tgggaaaact agtttctttt ttttcattga ttaattctaa atttgaaaag
    10621 gaaaatccgg gtgctattct atatttttga taagagtaac cagcataaat gattaagagt
    10681 agaatggata cgatgggaaa tatatttttt ttcatgattg aattcttttg caaagcaatt
    10741 ctttgtcaag tccacttatc cagttaccac gaaggaacac gaagttaggt tagaagtaaa
    10801 aaggaaaacc cttcgtgctt attccaccct tcgctccaag tattcacgat cagcttcgca
    10861 tgtgcgctt
//
LOCUS       DAOQOS010000155        10843 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_3145_length_10843_cov_6.197812, whole genome shotgun sequence.
ACCESSION   DAOQOS010000155 DAOQOS010000000
VERSION     DAOQOS010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 10843)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10843)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10843
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3145_length_10843_cov_6.197812"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(83..868)
                     /locus_tag="PLF66_22690"
     CDS             complement(83..868)
                     /locus_tag="PLF66_22690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020772906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMZ66914.1"
                     /translation="MSETPIIEMRNVHKTFGTRKILKGVDLYVKKGETMVILGPSGTG
                     KSVTLKHITGLVAPDEGDCFIFGESISFSKEKVRERLRAKMGVLFQSGALINWLSVYE
                     NVALPLREHKISKGEELHRNVMEKLQWLDLVVAKDNLPGDISGGMKKRAGIARALTTN
                     PEILLYDEPTSGLDPVMSNVFNELILRLKKELHVTSIVVTHDMESAYMIADRITFLYE
                     GKAIFCGTVSELKASPNPVIQQFIHGKTVGPMILDHSEKPKKH"
     gene            complement(872..1645)
                     /locus_tag="PLF66_22695"
     CDS             complement(872..1645)
                     /locus_tag="PLF66_22695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008590801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMZ66915.1"
                     /translation="MMALYDLAKSYIYGAGYTLVLLFETILQFRAIFFKRNEIVQQMF
                     TAGISSFFVVSIVAVFTGMIMGLNAGLGLKDFGAEGQIGLLLTITLTREMSPFMTALI
                     LSASVGSAIAAEIGTMKVSEEIDALEVMSISPVRYLVMPRVLGFSLMVPVLCIYSSIL
                     GVLGGAVVGHFQLGVEYQVYFNDVLLRVGSIPGLKDLYVGLLKGFVFGVTVSIVACSH
                     GLRATNGALGVGRATRESVVTSFLLVIFFGYVLTAVLYR"
     gene            complement(1653..3050)
                     /locus_tag="PLF66_22700"
     CDS             complement(1653..3050)
                     /locus_tag="PLF66_22700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66916.1"
                     /translation="MRKFLLFILFLLVSLHLEAKLPTYPNGIYPSNYWQFWVFYEKEW
                     RQGQKDIILRPFYSSYKDEMSAHTFNTSFYPIYYSEKTNHWNRWTFLFVFSHDSTLHD
                     DTGDDTDLVLSPLFQWGRGETEKDRYVAFFPFYGKMKSKLSWGEINFFLFPLYVDWSH
                     KEFKAYSFLYPLILYGKSDVRTEMRFLPLYSKKSHIAKFEHYTILWPFVQWGRDSLDK
                     KEPTSYSFFFPFYNLKSSQYGNMKSYAFLWFPILGSLVGYGYDRRTSEVNYNFLFFFL
                     QYGYSNSKDYRKHMFFPFYGYSRFASKEFTFITPLFVHMRSDTYGVKSNSYYMIPFFY
                     YMEKYFVKEERTDTYYKFWPVFKWHRDSEGNLFWNTLSIFPIRSTTLERIWDPFLSVL
                     EYKQFINGEKRLSLFMRLYTQRWTEDEFHLYVPFLLDLSLEKEKTSWKFLYGFLGYER
                     EQEKRQLQFLWFISI"
     gene            complement(3095..3167)
                     /locus_tag="PLF66_22705"
     tRNA            complement(3095..3167)
                     /locus_tag="PLF66_22705"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3132..3134),aa:Ala,seq:tgc)
     gene            3497..3772
                     /locus_tag="PLF66_22710"
     CDS             3497..3772
                     /locus_tag="PLF66_22710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010515454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66917.1"
                     /translation="MEWEKILRDAVKDNTIKELYLKKVPSLKTCEDWKQVKEVGLVDH
                     KTKYAHYKGGLVKYGDRLYFVIDQRLEAIAPFRAWNFKTKIKVTDPE"
     gene            complement(3776..5776)
                     /locus_tag="PLF66_22715"
     CDS             complement(3776..5776)
                     /locus_tag="PLF66_22715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017852164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HMZ66918.1"
                     /translation="MDKENILIQELGKLELPELKKVATLWNIHKFTGKDKKSSVSQLY
                     SAFQEEFYLKAILEKLTPIQVNIFSLILKNKNVQTLGEIVRKTSLHPINAETELGVLR
                     KYCLVYQRKNRERLTNNLDKYHAYSEIADLIKLDINAKGEKFKLSLDKYFHKHPVDQI
                     APEWIKVLGYKKIDSLDEFVKFGLSEEGIGMTIQSLSEIERDVLLYVYTHGGVIEAEV
                     VRNYISVNREKFEHVIPLLTSKYLVFDTYYVDEKFIRIITIPKEILNYIQVHTLLPSA
                     KKGTKQKQEKIAKNDLDFFLNIKKMISFISRKGLSLAKSGKIKQADNKRTEQDLLKPD
                     IDIFPEKGQVYQIELILPILKILNLVDIKGENIVLTGEIDEFQKKDIFELMNIVIHEV
                     NEVRSKRFNPPEVFSAIEVPFYDKPILDKCVNIILSMGKVNTSVIFSNVIRENLVLSP
                     GFRIKNFENDLMDLRKEIISAIFYLHLFGLLEVEYPSRYLVLSDLGKYYFQSKAIPKH
                     TEKGGITINSDFSIIAFPEKCSLHGIHLLKVFTELKDYDRVYTFALTKDSFQNGILLG
                     YDPNLFIQFLKDSSKAELAQNLMFLFDDWSKNLPIVVVTEDCVLLKTYDVHVMELLLG
                     QIKGKKIVMEEISPEAILIDKTKIQDVIQIAEKLNLIIKLIR"
     gene            5904..7226
                     /locus_tag="PLF66_22720"
     CDS             5904..7226
                     /locus_tag="PLF66_22720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016543816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HMZ66919.1"
                     /translation="MTQITDNIASGINAILRIADSFRILSTKLAQVLADILLDKKDKQ
                     LPVRLREAFEDLGATYIKLGQFIASAPSLFPKDYVDEMQKCLDSVRPIPFKTILQIIE
                     KELGDKPEKFFTFIEEKPMASASISQVHAATTVDGFDVVLKVQRPDIEDVLKTDMNLI
                     YLATLLFEKLSPGVKTSGLTDIVKTFHDSILLEVDFIQEAKNIEEFDKHLLNTNETRA
                     KVPRVFSKYSTKRLLTMERFYGIPLTDLKAIKTVSSNPAKTLTDALDIWFSSLGSGMF
                     HADVHAGNLLVLKDGKIGFIDFGIVGRISPDTWMGLMFFMEGLGTTNAKMMARGLVQM
                     DSTAKGIDETKFAKDLEYIFDEMNEIAMSIQLGDVAGIDETRLNGVMLRISDISKNNG
                     LKIPHEFGLLIKQMLYFDRYVKILAPEMDLIKDLTPMEAKKNRSIGYR"
     gene            7254..7499
                     /locus_tag="PLF66_22725"
     CDS             7254..7499
                     /locus_tag="PLF66_22725"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HMZ66920.1"
                     /translation="MLKSLSGIELNQKAIIDSMNQEKLNSDYILELLDFGFLPGTEIS
                     VLQKYPSQNKIVVQIGRAHLSLRLNDASFIQVRTNGN"
     gene            7489..9573
                     /gene="feoB"
                     /locus_tag="PLF66_22730"
     CDS             7489..9573
                     /gene="feoB"
                     /locus_tag="PLF66_22730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010418789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HMZ66921.1"
                     /translation="METSYRCLLVGNANAGKTSVFNRLTGLSQKTGNFPGVTVGIATG
                     TISSNDRELELIDLPGSFSLNANSEDKKTLTRFLMSRKPTDKIVFVLDAMLLERSLQF
                     LFQIMDLGAPLLLVITMKDILEKKNISIDLDKLKNELGIEVILVNGKTGDKIPELKEL
                     LLKESSFKTPERLWAWDEKRETFFKNLLTRIEADNKHYLSFVLSNALKKLSGEKLQKE
                     LPGIEEFNPTIQNFIQMELVASGLLFKYQEEVIYKSFKIKSIISHVLNGNIENQKSKS
                     AALDKIVLHPFFGMITFFAIMALVFQSLFSWSELPMEWIEKTFDTLANYFEKSLPIGP
                     LNGLLTKGIIGGVGSVLVFIPQISLLFLFIGIMEESGYLSRVSFIMDKFMGKFGLSGK
                     SFIPLLSSAACAVPAIMSTRTIENKSDKMTTILISPLITCSARYPVYILVIGAIFPKE
                     NVFGIFSLQALILFGLFILGMGTALLFALLFKKTFFKHESSYFLIELPSYKIPSVKNL
                     SLSVYQNVKAFIVNSGTIILYVSIVLWFLAYYPVNVGSEGAETKSEISESYAARLGKT
                     IEPVLQPLGFDWKIGVGIISSFAAREVLVSTLAIIYGVEGDETSDDLKTSLQSDINPK
                     TGKKTWSILTAISLLIFFAYASQCMSTLAVVRQETKSFFWPAFLFVYMTILAYFSSLA
                     VYQIGTALGFGN"
     gene            complement(9576..9896)
                     /locus_tag="PLF66_22735"
     CDS             complement(9576..9896)
                     /locus_tag="PLF66_22735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HMZ66922.1"
                     /translation="MTKNKSDLNKKLIEGTVKGIQGIAHPVRLMILHALSKEELSVND
                     LADYSGVSQSVTSQHLGKMKDIGILDSRKESNKVFYYIKDNRYRELSRSIGKLFSLYS
                     VSQK"
     gene            10103..10471
                     /locus_tag="PLF66_22740"
     CDS             10103..10471
                     /locus_tag="PLF66_22740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66923.1"
                     /translation="MRIKFPTRVRFTKMYLREIQTLRKQIPKNKDNSKINRYSTLSNF
                     EFLTRETTFESIREMKLYQERLKACLDNIFKICKIEKLTLEEKNRILNAFSNIQKNPN
                     TKDLGMYYRIRMNLVRYSLI"
BASE COUNT         3763 a         1866 c         1744 g         3470 t
ORIGIN      
        1 aataggtata tttagaagtt aaaaattcat ctactcgctt gttctgatct tttgattgaa
       61 ctctataaaa aaattgcatg tatcaatgtt ttttgggttt ttcagaatga tctaggatca
      121 taggaccaac tgtttttcca tgaataaatt gttgaatcac tggattcgga gaagctttta
      181 attcagaaac agttccacaa aaaattgcct ttccttcata taagaaagta attctatctg
      241 caatcatata agcactttcc atatcgtgag taacaacaat tgaagtgaca tggagttctt
      301 tttttaatcg aagaatgagt tcattaaaga cgttagacat cacaggatcc aatccggatg
      361 taggttcatc ataaagtaaa atttcaggat tggttgtaag agctcttgca atacctgctc
      421 tttttttcat ccccccactg atatcccctg gcaaattatc tttagccaca actagatcga
      481 gccattgtaa tttctccatc acatttcgat gtagttcttc tccttttgaa attttatgtt
      541 ctcttaaagg aagggctaca ttttcatata cactgagcca attgataagt gcacctgatt
      601 gaaataaaac tcccatcttg gctctaagtc tttctctaac tttttccttc gaaaaggaga
      661 tcgattctcc aaaaataaaa caatccccct catctggcgc aactagtcct gtaatgtgct
      721 ttagggtaac ggactttcca gttccagaag gtcctagtat gaccattgtt tcccccttct
      781 tcacatatag atcaacaccc tttaaaatct ttctagtgcc aaaagtctta tgcacatttc
      841 tcatttcaat aattggagtt tcagacatat tttacctata tagaactgcg gttaatacat
      901 aaccaaaaaa tataactagt aaaaaagaag tcacaacgga ttctctagtg gctctcccta
      961 ctcccaacgc accgttagtc gctctaagtc catgggaaca agcgacaatt gagacagtaa
     1021 ctccaaatac aaaacctttg agtagcccta catataaatc tttaagacca ggaatagaac
     1081 caactctaag aagcacatca ttgaaataaa cctgatattc cacgcccaac tgaaagtgac
     1141 caacaacggc tccccctaaa actccaagga tactggaata aatacaaaga accggaacca
     1201 tcaaagaaaa tcccaatact ctaggcataa caagataacg cacagggcta atcgacatta
     1261 cctccagagc atcaatttct tcggacactt tcatagttcc aatttcggca gcaatagcag
     1321 aaccaacaga agcagacaaa ataagagctg tcataaaagg agacatttca cgagtgagag
     1381 taatcgtgag taaaagaccg atttgcccct ccgctccaaa gtctttcaag ccaagtcctg
     1441 cattaagacc catgatcatt cctgtaaaaa cagcaacaat ggatacaaca aagaaactac
     1501 taatccctgc ggtaaacatt tgttggacaa tttcatttct cttaaaaaaa attgctctaa
     1561 attgtaaaat ggtttcaaat aataatacaa gtgtatatcc cgcaccatag atataactct
     1621 ttgctaaatc atataaagcc atcatttaaa ctctaaatag aaataaacca gagaaattgg
     1681 agctgccttt tctcttgctc tctctcgtaa cctaaaaatc catataaaaa cttccaagaa
     1741 gttttttctt tttctaatga caaatcgagt aggaaaggaa catagagatg aaattcatcc
     1801 tctgtccatc tctgggtata aagtctcata aatagagaaa gtctcttctc cccattgata
     1861 aattgtttgt attctagaac agataaaaag ggatcccaga ttctctcaag agttgtggag
     1921 cgaattggaa aaatagataa agtattccaa aaaagatttc cttctgaatc tctatgccat
     1981 ttaaatacag gccagaattt ataataggtg tctgttcttt cttcttttac aaaatatttt
     2041 tccatatagt aaaaaaatgg aatcatataa tatgaattgg attttactcc atatgtatca
     2101 cttctcatat gaacaaagag tggagtaata aaagtaaact ctttactggc aaatcgggag
     2161 taaccgtaaa agggaaaaaa catatgttta cgataatcct tactatttga atatccatat
     2221 tgtaggaaga aaaagagaaa attataattg acttcactcg ttcgtctatc gtatccatat
     2281 ccaacaagtg aacctaaaat tggaaaccat aaaaaagcat aacttttcat atttccatat
     2341 tgagatgatt ttaaattata aaaaggaaag aaaaaagaat aagaagtcgg ttctttttta
     2401 tcaagggaat ctctacccca ttgaacaaaa ggccatagaa tggtataatg ttcaaactta
     2461 gctatatgag attttttaga ataaagagga agaaacctca tttcagttct aacatccgat
     2521 tttccatata aaatgagtgg atataaaaag ctataggctt taaattcctt atgactccaa
     2581 tctacgtaga gtggaaataa aaagaaatta atttctcccc aagaaagttt tgatttcatt
     2641 ttcccataaa agggaaagaa tgccacataa cgatcttttt ctgtttcccc tcttccccac
     2701 tgaaagagtg gagacaaaac tagatcagta tcatctcctg tatcatcgtg aagagtgcta
     2761 tcatgactaa atacaaataa aaaagtccac cggttccaat gatttgtttt ctcagaataa
     2821 taaattggat aaaaggaagt attaaaagta tgtgcactca tctcatcttt gtaagaggag
     2881 tagaaaggac gtaagataat atctttttgc ccttgcctcc attccttttc atagaatacc
     2941 caaaactgcc aatagttcga tggataaatt ccattcgggt aagtaggtaa ctttgcctct
     3001 agatgaagcg aaacaagcag aaataagatg aataacaaaa acttacgcac tatagtaatt
     3061 tctattagag agtataggta ggcaataaaa ataatggagc ttatcgggat cgaaccgatg
     3121 accctctgct tgcaaagcag atgctctccc agctgagcta aagccccttt tatgaatcct
     3181 agggatgtat taccattctt ttaatcttgt taaaattgaa aagtagtttt gctaaatctt
     3241 ttgcatcctt ttgaaacctg aaagatattt ccaaaaatac aaagcttttt acaaataaac
     3301 attgactact ctatgaagaa ttatatagta ccaatttgca gcttatctgc taaatacaga
     3361 taaaaaaaca gagatgtttt tataaaaatg gtgtttagta aggcaaattt attttttaga
     3421 tcagaaatat tctgaatctg gtcgatcgat ataagaaata aaataagcta agaatacagt
     3481 aaggaatctg ttctttatgg aatgggaaaa aattttaaga gacgcagtta aagataatac
     3541 aatcaaagag ttatatttaa agaaagtgcc atccttgaaa acatgtgagg attggaaaca
     3601 agtaaaggaa gttggattag tagatcataa aacaaagtat gcccactaca aaggtggact
     3661 tgtgaaatat ggggacagac tttacttcgt aattgatcaa cggcttgaag ctattgctcc
     3721 ttttagggcc tggaacttca aaaccaaaat caaagttaca gacccagaat aggaattatc
     3781 taattagttt aataattagg tttaacttct ccgctatttg aataacgtcc tggattttag
     3841 tcttatcaat taagattgct tcaggactta tttcctccat aacaatcttc ttccctttaa
     3901 tctgcccaag caataattcc attacatgaa catcgtaggt cttaagtaat acacaatcct
     3961 cagtaacaac aacaatcggt agattcttgc tccaatcatc aaataggaac atcaagtttt
     4021 gagcgagttc tgctttgcta gaatctttta agaattgaat aaatagattc ggatcatatc
     4081 ctaataaaat accattctga aatgagtcct tcgttaaagc aaaagtatat actcggtcgt
     4141 agtctttgag ttctgtgaat acttttagta aatgaattcc atggagtgaa catttctcag
     4201 ggaatgcaat aatactaaag tcagagttaa ttgtaattcc acccttctca gtatgtttag
     4261 gaatcgcttt tgattggaaa taatattttc ctaaatcaga aagaactaaa tatctagaag
     4321 gatactctac ttctaaaaga ccaaataaat gaagatagaa aatagcactt atgatttctt
     4381 tgcgtaaatc cattaaatca ttttcaaagt ttttaattct aaatccagga gataaaacta
     4441 agttctcacg gataacgttt gaaaagatta cagatgtatt tactttcccc atacttagaa
     4501 tgatattcac gcatttatct aaaataggtt tatcataaaa cggaacttcg attgcagaga
     4561 aaacttctgg aggattgaaa cgcttagaac gaacttcatt tacttcatga atcactatat
     4621 tcattaattc aaaaatatct ttcttttgaa attcatcgat ctcaccagtt aaaacaatgt
     4681 tttccccttt gatatctact aaatttaaaa tttttaaaat aggtagaatc aattcgattt
     4741 gatagacttg tcctttctct gggaaaatgt caatgtctgg tttcagtaag tcttgttctg
     4801 ttcttttatt gtctgcttgc ttgatctttc cagacttagc aaggcttaag ccttttctgg
     4861 aaatgaaaga aatcatcttt ttaatattta aaaagaaatc taaatcattc tttgcaatct
     4921 tttcttgctt ttgtttcgtt cctttctttg cggaaggaag aagggtatgc acttgaatgt
     4981 agtttaaaat ttcttttggg attgtgatga ttcgtataaa tttctcatct acataatagg
     5041 tatcaaacac caaatacttg gaagttagaa gaggaattac atgttcaaac ttttctcgat
     5101 ttacactgat ataatttcga actacttccg cttcaatcac tccaccatgg gtatatacat
     5161 agagtaaaac atctctttca atttcagaaa gagattggat tgtcatcccg attccttctt
     5221 cagacaaacc aaattttaca aattcatcta aagaatcaat tttcttataa cctagaactt
     5281 tgatccattc tggagcaatt tgatcaactg gatgcttatg aaaatattta tcaagggaga
     5341 gtttaaactt ctcccctttt gcattgatat ctagcttaat taaatctgca atttcggaat
     5401 aagcatgata tttatctaaa ttgtttgtta acctttctct attttttctt tgataaacaa
     5461 gacaatattt ccttaaaaca ccaagctctg tttccgcatt gattgggtgc agagaagtct
     5521 ttctaacaat ttccccaaga gtctgaacgt ttttattttt taggatgaga gagaatatat
     5581 tcacctgaat cggtgtgagc ttttcaagta ttgcctttaa atagaattct tcctgaaatg
     5641 cagaataaag ttgggaaaca gaggatttct tatctttgcc tgtaaatttg tgaatattcc
     5701 aaagagtggc tacttttttt agctcaggga gctccaattt cccaagctcc tgaattaaaa
     5761 tgttttcttt gtccatagat ggttttttta ttacttcttt ttagaaattt ctgacaatgt
     5821 ttgtcaaaat tgttattctg taaacaaaaa atcgttctaa cgaaatgaat tgtcagtata
     5881 accctacttg aaaatttatc cctatgactc aaataacaga caacattgca tccggaatca
     5941 atgcaatcct tcgaatcgca gactcattta gaattctatc tacaaaatta gcacaagttc
     6001 tagcggatat tctactagat aaaaaagata aacaactccc agtaagactg agagaagcat
     6061 tcgaagattt aggagctacc tatattaaat tagggcaatt tatagcctcc gctccatctc
     6121 tttttcctaa agattatgtt gatgaaatgc aaaagtgttt agactctgtt cgccctattc
     6181 cttttaaaac cattttacag attatcgaaa aagagttagg agacaagcca gagaaatttt
     6241 ttacctttat tgaagaaaag ccaatggcat ctgcctctat ttcacaagtg catgcagcaa
     6301 ctactgtaga tggtttcgat gtagttctaa aagtgcaaag acctgacatt gaagatgttt
     6361 tgaagacaga tatgaatcta atttatcttg caacactact ttttgaaaag ctttctccag
     6421 gtgtaaaaac atcaggactt acagacatcg taaaaacatt tcacgattcc attttgcttg
     6481 aagttgattt tatccaagaa gcaaaaaaca tcgaagagtt cgataagcat ttactcaaca
     6541 caaatgaaac gagagctaaa gtgccaagag tgttttctaa atacagcaca aagcgtttac
     6601 ttacaatgga aagattctat ggaattccac tcactgatct aaaagcaatt aaaacagttt
     6661 caagtaatcc tgcaaagact ttaacagatg cgttagacat atggttctca tcccttggta
     6721 gtggaatgtt tcatgcggat gttcacgcag gaaatttact tgttctaaaa gatggaaaaa
     6781 taggttttat cgattttggt atcgtaggaa gaatttctcc tgatacttgg atgggtctca
     6841 tgtttttcat ggaaggtctt ggaaccacta acgcaaagat gatggcaaga ggtctagtgc
     6901 aaatggattc aaccgcaaag ggaatcgatg aaactaaatt tgcaaaagac ttggaataca
     6961 tttttgatga aatgaatgaa attgctatga gtatacaact cggagatgtt gctggaattg
     7021 atgaaacaag actcaacgga gtcatgttac gaatcagtga tatcagtaaa aacaatggac
     7081 ttaaaattcc acatgagttc ggacttctga ttaaacaaat gctctacttt gatagatatg
     7141 tgaaaatttt agcgccagaa atggatttaa taaaagactt aactccgatg gaagcaaaaa
     7201 agaatcgctc tatcggatat cgatagtttc tatttccaga acaggaatta aatatgctta
     7261 aaagtttatc agggatagag ctgaatcaga aagcaattat tgattctatg aatcaagaaa
     7321 aattaaactc tgactatatt ttagaattac tcgactttgg ttttttgcct ggcacagaaa
     7381 tctctgttct gcaaaagtat ccaagtcaaa ataaaatcgt agttcaaatt ggtagagcac
     7441 atctttctct tcgcttaaat gatgcttcct ttattcaggt aagaacaaat ggaaactagt
     7501 tatcgttgtt tactcgtagg aaatgcgaat gcagggaaga cttctgtatt taatcggcta
     7561 acaggtctga gtcaaaagac aggaaacttt ccaggagtga ctgtcggaat cgcaacggga
     7621 acaatttcat ccaatgatag agaacttgaa ctaattgatt tacctggctc cttttctttg
     7681 aatgctaatt ccgaagacaa aaaaacactg acacgatttc taatgagtcg aaaaccaaca
     7741 gataaaattg tattcgtttt ggatgcaatg ttactagaaa gaagtttgca gtttttattt
     7801 caaatcatgg atttaggagc cccgcttcta cttgtaatca caatgaaaga catcttggaa
     7861 aaaaagaata tttccataga tctagataaa ttaaaaaatg agctaggaat tgaagtgatc
     7921 cttgtaaacg gaaaaacagg ggataaaatt cctgaactaa aagaattatt attaaaagaa
     7981 tccagtttta aaactccaga aagactttgg gcatgggatg aaaaaagaga aaccttcttt
     8041 aaaaacctac tgactagaat agaagcggat aataagcatt atctctcttt tgttttatca
     8101 aatgcactaa aaaaattgtc tggtgaaaaa ttacaaaaag aattacctgg gatcgaagag
     8161 ttcaacccta ccattcaaaa ctttatccaa atggagttag ttgcatcagg tttattattt
     8221 aaataccaag aagaagtcat atataaatct tttaaaatta aatctattat tagccatgta
     8281 ctcaatggca atattgaaaa ccaaaaaagt aaatcggcag ctttagataa aattgtttta
     8341 catccttttt ttgggatgat tacctttttt gcaattatgg cgcttgtttt ccaatcctta
     8401 ttttcttggt cggaactccc aatggagtgg atagaaaaaa catttgatac gctcgccaat
     8461 tatttcgaaa agagcctccc aatcggtcca ttaaatggtc ttctcacaaa aggaatcatt
     8521 ggaggagttg gttctgttct agtatttatc ccacagattt cgttattgtt tttatttatt
     8581 ggtatcatgg aagagtctgg ttacttatct cgcgtatcgt ttataatgga taaatttatg
     8641 ggtaaatttg gtttatctgg taagtcattt atacctctac tttcttctgc tgcttgtgca
     8701 gttcctgcaa tcatgagtac tagaacaatc gaaaataaat cagacaagat gactactata
     8761 ctaatttctc cacttataac ctgttccgca cgttatccgg tatatatact tgtcattgga
     8821 gctatctttc cgaaagaaaa tgtttttggg attttttcct tacaagcact cattttattt
     8881 ggattgttta tcttaggaat gggcacagct ttactttttg cccttttgtt taagaaaact
     8941 tttttcaaac atgagtcttc ttatttttta attgaactac cttcatataa aattccatca
     9001 gttaaaaatt tatctctcag tgtttaccaa aatgtgaaag cattcatagt aaattcagga
     9061 acgattattc tctacgtatc tattgttctt tggtttttag cctattatcc agtgaatgta
     9121 ggtagcgaag gagctgaaac taaaagtgag atttctgaat cttatgcagc aagacttgga
     9181 aaaaccattg aaccagtttt acaacctctc ggtttcgatt ggaaaattgg agttggaatt
     9241 atatcttctt tcgcagcgag agaggtgctt gtttcgaccc ttgcaattat ttatggagtg
     9301 gagggagatg agacaagtga tgatttgaag acatcattgc agtctgatat aaatccaaaa
     9361 acgggaaaaa aaacttggag tattttaaca gcgattagcc tgctcatttt ctttgcttat
     9421 gccagccaat gtatgtcaac gttagctgtt gttagacagg aaacaaaatc ttttttctgg
     9481 cctgccttct tatttgttta tatgacaatc ctagcatatt ttagttctct agctgtttat
     9541 caaatcggca ctgcattggg ttttggaaat taaatttatt tttgtgaaac agagtataga
     9601 ctaaaaagtt tcccaatgct acgagaaagc tctctataac gattgtcttt aatataataa
     9661 aaaaccttat tggattcttt gcgactgtct agaattccga tgtctttcat cttacctaaa
     9721 tgctgagatg tgacagattg actaacgcca ctataatcag ctaaatcatt cacagagagt
     9781 tcttccttag agagagcgtg caaaatcatg agccgaacag ggtgcgcaat tccttggatg
     9841 ccttttactg ttccctctat aagtttttta tttagatccg atttattctt tgtcatgttt
     9901 ttatctcgaa agcacctagg gtttgaaaca atctagagtt taaggcattt attttcaata
     9961 ttattttcag caaaaaaatt gaattaaaga tctattttta ccataggttt tccttacaca
    10021 ctcctttgat aaaaatgcaa aataaaataa aaaaaaataa taagaaattt aaatcaaaaa
    10081 tttttctttg acaaaaatta aaatgcgaat aaaatttccg actcgtgtga ggtttacaaa
    10141 aatgtatttg agagaaatac aaacgttaag gaaacaaatc cctaaaaaca aagataattc
    10201 taaaatcaat cgatactcta ctctctcaaa ttttgaattt ttaacgagag agacaacctt
    10261 tgaaagcatt cgagaaatga aactttacca agaaagactc aaagcatgtt tggataatat
    10321 tttcaaaatc tgtaaaatag aaaagctcac actagaagaa aagaatcgga ttctaaatgc
    10381 attctctaat atacaaaaaa atccaaacac aaaagactta ggaatgtatt atagaattag
    10441 aatgaatctg gttcgctaca gtttgattta aaattatttt tagcgttata atagatctga
    10501 attgagtttt ctaaaattct atttcattct ttacagaaat ttttaaaaag aaattcctga
    10561 gaatatatta aaactaagaa aactttgatg tattccagtt ggatatgaaa ccatttggta
    10621 atacgctaaa tctctcaaac tagggattct acttttatct ataagaccag gctcgtcaga
    10681 aaaaacataa aagtttcgat gtggattcaa ttctttcgca attcctaaat caaaaccaaa
    10741 aagtaaccca aaatcaaaat cccatctcca tccaaaggaa aagttcgcat aatcagtctg
    10801 ttgcgaaata agaaaaaggg tttacagaat agattttaat aaa
//
LOCUS       DAOQOS010000156        10834 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_3150_length_10834_cov_5.824474, whole genome shotgun sequence.
ACCESSION   DAOQOS010000156 DAOQOS010000000
VERSION     DAOQOS010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 10834)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10834)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10834
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3150_length_10834_cov_5.824474"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..647
                     /locus_tag="PLF66_22745"
     CDS             <1..647
                     /locus_tag="PLF66_22745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010413219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SbcC/MukB-like Walker B domain-containing
                     protein"
                     /protein_id="HMZ66924.1"
                     /translation="ALENIDYIPGYTFIQLDCQPTANVEILDFKKELIDCIPDLEKPE
                     ENESKFIKIRDLLDKLKNRSSENTDRWVKLVTDVRNWLDFRAIELTRDDRKQKEVYDS
                     SAGKSGGQTVKLAYTILASAISYQFGIREKKSFRLVVIDEMFNNLDNQNSRFAMDLFK
                     QLGLQLLVVTPLDKISIVEPYISSIHLVANNQEGKDSRVYPITMETLRKKKEGS"
     gene            complement(644..1141)
                     /locus_tag="PLF66_22750"
     CDS             complement(644..1141)
                     /locus_tag="PLF66_22750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012388381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HMZ66925.1"
                     /translation="MAQDPSFDIISKVEKSELANAVTQALSEINTRFDFKGSKSDIKV
                     EEENLVITSDNDIKIKQVIDVLINKLAKRGVGLKAFNFDSKIESATGNTARMKVKIQQ
                     GLEKEHTKEIVRIIKDSKLKVQAAIMGDYVRVTGKKKDDLQEVQHLLKKNELNFDFTF
                     TNYKG"
     gene            complement(1146..3893)
                     /gene="alaS"
                     /locus_tag="PLF66_22755"
     CDS             complement(1146..3893)
                     /gene="alaS"
                     /locus_tag="PLF66_22755"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HMZ66926.1"
                     /translation="MYKTVAEIRKLFIEYFRANGHTIVPSSSLIPVGDPTLLFTTAGM
                     VQFKPYFTGAVELTFTRAASSQKSLRTTDLENVGRTERHCTFFEMLGNFSFGDYFKKE
                     AISYALEFSTKHLGFDKDKIWITVYLDDDEAENFWIEAGIPKERIVRLGKEDNFWGPA
                     GDSGACGPCSELYLDRGVSKGGKDCGKLNTCKPGCSCDRFLEFWNLVFNQFDQDITGK
                     LNPLKQTGIDTGSGLERVALLLQNVDSVYDTDELRRIIGFIEKLSGRKYEADTKTPFR
                     VLTDHSRAITFAITDGVMPDKTGRGYVIRRLIRRASLFARRIGIKEAFIYKVIPEIVE
                     IYKLEYPDLEKRKNEVIRIVKSEEDLFLNTLELGLGVIESMAKVYESKNEKTFSGADI
                     FKLYGTYGFPVEMTKEILRDKGFAFDEKGYLEELEKDRKLSRESWKGKKAQILTSIGE
                     KISPTQFSGYEKLSEQGKILYIIKDSKTKSEIQSGDEAVLVLDKSCFYGESGGQIGDS
                     GYIKLGDNVFQVKDTQKENDVILHIGAMLSGQLKLGDLVSLEIDTTKRELLTYHHSGT
                     HLLNGALREILGSHVAQKASLVSDEYLRFDFSHNAALSEEEILKIESKVNEAIQSSIP
                     VATKILSLDEAKKTGAVAFFEEKYGDIVRVIQMGKYSIEFCGGTHVSNTGNIKYFYIQ
                     KESSPGAGNRRIEAVCGSPVIEFFQKEFQTLTQKVQEFNLKAKDVFKDSKILIDTKIP
                     SPDEVQSSFLTNGANSVRLYRDLKAKIEKDLANKTIELNKEKKRLEAAGNSDILSQSD
                     EILKNSVKINSILFVSKVFKDLKIDVLKELGDKIKNQQEKIFISFLLESASNKTILLM
                     ANKNAVSSGFDCNQILKATTSAIGGKGGGKIDMAQGSIGSDINNDVIIETIKKELQAR
                     G"
     gene            complement(3902..4372)
                     /locus_tag="PLF66_22760"
     CDS             complement(3902..4372)
                     /locus_tag="PLF66_22760"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HMZ66927.1"
                     /translation="MDEKLYSKILNKIKVRERSEKEILEFLEKSGVSSNDSAFVIDRL
                     KNSKLLDNNRFAESKIRSRIYNQHWGIEKIRYELIEFGVPKDLIEEKLLEVDPSVWVL
                     NCEKIISKNKLNKENQMEIFKLKKKLFQMGYPNQTIKQAFRNQEIKEEIEVDLE"
     gene            complement(4382..4780)
                     /gene="rpsI"
                     /locus_tag="PLF66_22765"
     CDS             complement(4382..4780)
                     /gene="rpsI"
                     /locus_tag="PLF66_22765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HMZ66928.1"
                     /translation="MAKDKSTWTVGRRKTSVARLKLTDGTGKITVNKKDIKDYIKTGE
                     AKVAAAIAPLTILNVRDKYDLQLNVHGGGFTGQVEAIRHAIARALSLKSAESRSILKK
                     EGFLTRDSRMVERKKYGLHKARRGTQFSKR"
     gene            complement(4798..5247)
                     /gene="rplM"
                     /locus_tag="PLF66_22770"
     CDS             complement(4798..5247)
                     /gene="rplM"
                     /locus_tag="PLF66_22770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017857686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HMZ66929.1"
                     /translation="MPIISPAHRTPSLKKEQIKKNWFVINAEGKTLGRLCSAVAARLR
                     GKHKATFTPNQDCGDNIIIINAAKVQVTGRKRDQKIYYHHSLHPGGMSAIPFKDLIQT
                     NPERVLMEGVKGMLPKSKLGDVMLSNLRVFAGSEHNLKAQKPVAIEI"
     gene            5938..6840
                     /gene="thiL"
                     /locus_tag="PLF66_22775"
     CDS             5938..6840
                     /gene="thiL"
                     /locus_tag="PLF66_22775"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HMZ66930.1"
                     /translation="MNEEKIIRHLYKNQSFDPFVDDCFWMDKKYLVTTDTMCEGTHFK
                     HEWSSPKDLACKLVTLNVSDLFSSGGIPKYAFLNLGLSKISSNSKWLSSFTNALRKNL
                     QKYNIILSGGDTYRSQTTNLNLTLIGTINHLIQRKGASVGDKIFITGKIGLSILGLKY
                     LENNIDLPLRFKKAALKKHLAPEVRNLFAKNPSIYKNMTAMMDISDGLIQDANKLAKA
                     SNVHLNIHIEKIPFFVNYEKYLTIDDALNSGEEAEYLFTGDVNLKKSKFITLIGEVLS
                     GNAGVSFLKDNKKYSPKEKGFLHF"
     gene            complement(6860..7966)
                     /locus_tag="PLF66_22780"
     CDS             complement(6860..7966)
                     /locus_tag="PLF66_22780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HMZ66931.1"
                     /translation="MIKTSSNSRKYYSAKKKKVYTKETAPKINTNERRLITVNQTVIR
                     KKIISECQKTIKKIDKAQREIDQFQKIDKPAYDRWIQENFGEKIQKIRQSSIEIQEKR
                     ELIEEVQDEAFRQGITFVEAYKIVIEKRKNVNPKEEKEKTSTENENFWENNFEDEEDI
                     FREDLNEFFKRFNEKFNQSAEDFESIRKGFGLGRKGSEKDLDSRLKERYRIIVQKLHP
                     DKNRDASDLEKELWHEAQEAYKQKDLDKLDFIITMFNIKVGSIGIESSVFDLKSANSK
                     LMQKLKSIANSIKAAKKEPSWGFLLLKPIKLAYLKKEAEIEFREFEKMTQTELKEINA
                     ILAKWEKGDEERKQNFNRRRNVVEEDFFDFYVNF"
     gene            complement(7963..9096)
                     /locus_tag="PLF66_22785"
     CDS             complement(7963..9096)
                     /locus_tag="PLF66_22785"
                     /inference="COORDINATES: protein motif:HMM:NF013237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019343 - cysteine biosynthetic process via
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HMZ66932.1"
                     /translation="MHIDTILVHGGDAHKSVTGSISMPIYQTATFEHPELGKSTGYDY
                     SRTQNPTREKLENSYAILESGKFGFAFTTGMAAISAIFDLFKAGDEFIVCDDLYGGSY
                     RFFQEICKNRNQKFVYVDSSNVENIQKEINKNTKAIFIESPTNPMMKITDIKAVADLA
                     KEKNILTIFDNTFLSPFFQRPLELGMDIVICSATKFIGGHNDTLAGLIAVKDSNLGEK
                     IRFIQNTVGSGLAPFDSWLMLRGLKTLGLRMQRQEENANAIANWLLQHPLVEKVYYPG
                     IKTHPGHETSKKQSTGFGAMISFELKEQKLVEQILKRVKIIAFAESLGGVESLITYPI
                     VQTHAAIPKDILDKIGLTDRLLRLSVGIESKEDLIADLEQAMQ"
     gene            complement(9201..10133)
                     /gene="cysK"
                     /locus_tag="PLF66_22790"
     CDS             complement(9201..10133)
                     /gene="cysK"
                     /locus_tag="PLF66_22790"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015677941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HMZ66933.1"
                     /translation="MKAENILQTIGNTPHVKLNRLYGNKANVWVKLEKANPGGSIKDR
                     IALSMIEDAEKKGILKKDSIIIEPTSGNTGVGLAMVAAVKGYPLILVMPESMSIERRR
                     LMSAYGATFELTPREKGMPGAIEKAKELVASKKNAWMPQQFENDANIQVHINTTAKEI
                     LNDFSDGLDYLITGVGTGGHITGVAKVLKEKFPNLKVFAVEPAASPVITATLNNEPPK
                     PGPHPIQGIGAGFIPKNLDTKLLDGVIQITKEEAFEYAVRSAKEEGIFVGVSSGASLA
                     AVSKKINEIPAGAKVLTFCYDTGERYLSIEGLFA"
     gene            complement(10243..>10834)
                     /locus_tag="PLF66_22795"
     CDS             complement(10243..>10834)
                     /locus_tag="PLF66_22795"
                     /inference="COORDINATES: protein motif:HMM:NF014543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66934.1"
                     /translation="FDGEGTLILADGEKYVGNFANGMFDGDGVLSFPNGDKYDGEFKQ
                     GKFNGNGTLAFNSGERYEGRFKEGKFDGDGNLYFKSGSKYIGKFASGNYSGHGVLTFK
                     NGKKYVGDFKNNKFDGKGTLTFPNGTVYVGEFKEGLFHGQGTFDSYTGEKYVGEFKNG
                     TYNGKGTLTLPDGTAVAGEFLNGNVTGGEMTFTDKR"
BASE COUNT         3236 a         1997 c         1558 g         4043 t
ORIGIN      
        1 aggcattaga gaatattgat tatattcctg gttatacatt tatacaacta gactgtcaac
       61 caactgctaa tgttgaaatc ttagatttca aaaaagagtt aatcgattgt attcctgact
      121 tagaaaagcc agaagaaaat gaatctaaat tcataaagat tagagatcta ttagacaaac
      181 tcaaaaatag aagttctgag aatacggata ggtgggttaa attagtaact gacgttcgca
      241 attggttaga ctttagagcg attgaattaa ctagagatga tcgcaaacaa aaagaagtct
      301 atgattccag tgcaggtaaa tcaggtggac aaacagtaaa actagcttat actattttag
      361 catctgcaat ttcctatcaa tttggaatta gagaaaagaa atcgtttcgg ttagttgtga
      421 tcgatgagat gtttaataac ctagacaatc aaaattctag atttgctatg gacttgttca
      481 aacagctggg attacaattg ttagtcgtta ccccattgga taaaataagc attgtagaac
      541 cctatatctc ttctattcat cttgttgcaa ataaccaaga aggaaaagat tctagggttt
      601 atccaataac gatggaaacg ttaagaaaga aaaaagaagg ctcctaacct ttataatttg
      661 taaaagtaaa atcgaaattt agttcatttt tttttagaag atgctgaact tcttgtagat
      721 cgtctttttt cttgcctgtt actctaacat aatcgcccat gattgcagct tggactttta
      781 atttagaatc ttttataatt cttacgatct ctttcgtatg ttctttctct aaaccttgct
      841 gaatttttac tttcattcta gctgtgtttc cagttgcaga ctcaattttg gaatcaaaat
      901 taaacgcttt gagaccgact cctctcttag ctaatttatt aattaaaaca tcaatcacct
      961 gtttgatttt aatatcattg tctgaggtaa taactaaatt ttcctcttct actttaatat
     1021 cagacttaga acctttaaaa tcaaatcgag tattaatttc tgagagtgct tgtgttacag
     1081 catttgccag ttcagacttt tcaaccttag atattatatc aaatgaagga tcttgtgcca
     1141 ttttgttaac cccttgcttg taattccttt tttatagttt caatgataac atcgttgtta
     1201 atatcggatc caattgaacc ctgcgccata tctattttgc ccccaccctt gccgcctatc
     1261 gcactggttg tagcttttaa tatttgatta cagtcaaatc ctgaagatac agcattttta
     1321 ttcgccatga gtaaaattgt tttattcgaa gcagactcta agagaaaaga aataaaaatc
     1381 ttttcctgtt ggttctttat tttatctcct aactctttta atacatcaat cttaagatct
     1441 ttaaatactt tagaaacaaa aagaattgaa ttgattttaa ctgaattttt taggatttca
     1501 tccgattggg atagtatatc actgttccca gctgcttcta atcttttctt ttctttattt
     1561 aattcaatag ttttatttgc taagtctttt tcgattttgg cttttaaatc cctatataac
     1621 ctaacgctat ttgctccgtt tgttaaaaag gaagattgaa cttcatcagg acttggtatt
     1681 tttgtatcta ttagaatttt actgtccttg aaaacatcct tagctttgag attaaattct
     1741 tgcacttttt gagttaaggt ttggaattct ttctgaaaaa attctatcac aggtgacccg
     1801 cacactgctt caatacgcct atttcctgca ccagggctag attctttttg aatatagaaa
     1861 tattttatat ttccagtatt cgagacgtgc gttcctccac aaaattcaat ggaatatttc
     1921 cccatttgaa tcactcggac aatatctcca tacttctctt caaaaaaagc tacagcccct
     1981 gttttcttag cctcgtctaa acttaaaatt ttagtagcaa caggaataga agactgtatt
     2041 gcttcattta ctttagattc aatttttaaa atttcttctt ctgaaagagc tgcgttatgc
     2101 gagaaatcaa atcttaaata ttcgtctgat acaagagatg ctttttgtgc aacatgggat
     2161 ccaagaattt cccgtaaagc tccatttagt aaatgagttc ctgagtgatg ataggttaaa
     2221 agttctcttt tggtagtatc aatttctaaa gatacaagat cacctaactt taattgtccg
     2281 cttaacattg ctcctatatg taagatcaca tcattttctt tttgtgtatc tttcacttga
     2341 aaaacattgt cacctaactt gatatatcct gaatctccga tttgtccacc agattcacca
     2401 taaaaacaag atttatctaa aactaaaact gcctcatcgc ccgattgtat ctcactcttt
     2461 gttttagagt cttttatgat gtataaaatc ttcccttgtt cagagagttt ttcataacca
     2521 gaaaattggg ttggagatat tttttcccca atagatgtaa gtatttgtgc tttttttcct
     2581 ttccaacttt ctctagatag ttttctgtct ttttctaatt cttctaaata tcctttttca
     2641 tcgaaggcga aacctttatc tctgagtatt tcctttgtca tttctaccgg aaatccatac
     2701 gtgccataca atttgaaaat atcagcaccc gaaaaagttt tttcattttt ggactcataa
     2761 acctttgcca tagactcaat gactccaagt ccaagctcta aggtatttag aaataaatcc
     2821 tcttcagatt taactatgcg gataacttca tttttccttt tttctaaatc aggatattct
     2881 aatttgtata tttccactat ttcaggaatt actttgtata taaatgcttc tttaattcca
     2941 attcttctgg caaaaagaga tgctcttcga attagtctac gtataacgta tcctcttcca
     3001 gttttatctg gcataacacc atctgtaatt gcaaatgtaa ttgctctaga atgatctgtt
     3061 aaaacacgga agggagtttt agtatctgct tcgtattttc ttccacttaa tttctcaata
     3121 aaaccaataa ttcttcttaa ttcatctgta tcataaacag aatccacgtt ttgcaaaagt
     3181 aatgcaactc tctcaagacc agatcctgta tcaataccag tttgctttaa agggtttaac
     3241 tttcctgtta tatcttgatc aaactgattg aagactaaat tccaaaactc tagaaagcga
     3301 tcacaactac atcctggttt gcaagtattc agctttccac aatcttttcc acctttactg
     3361 acaccacgat ctagataaag ttctgaacaa ggtccacagg ctccagaatc ccctgcaggt
     3421 ccccaaaaat tatcttcttt tccgagacga acaattcttt ctttaggaat tcctgcttcg
     3481 atccaaaagt tttctgcttc atcatcatct aaataaactg ttatccagat tttatctttg
     3541 tcaaaaccta aatgctttgt tgaaaattct aaggcatatg aaattgcctc tttcttaaag
     3601 taatctccaa agctaaagtt tccaagcatt tcgaaaaatg tgcaatgtct ctctgtccta
     3661 ccaacattct ctaaatcagt tgtccttaat gacttctgag aactagcagc ccgagtaaaa
     3721 gttagttcca cagctcctgt gaaataaggt ttaaattgaa ccattcccgc tgttgtaaat
     3781 agtaatgtgg gatcccctac tggaattaag cttgatgatg gaactatcgt atgtccattt
     3841 gctcggaaat attctataaa taattttctg atctcagcta ctgttttata cataactcac
     3901 cttattctaa atctacttca atctcttctt tgatttcttg atttctaaat gcttgtttta
     3961 ttgtttgatt cggatatccc atttggaaaa gttttttttt caatttaaag atttccattt
     4021 ggttttcttt atttaattta tttttagaga ttattttttc acaattcaga acccacacac
     4081 tgggatctac ctctaataac ttttcctcga ttagatcttt tggaactcca aattcaatca
     4141 attcatatcg aatcttttcg attccccaat gctgattata aattctagat cgaattttac
     4201 tctctgcaaa acggttgtta tctaataatt ttgaattctt gagtctatca ataacaaaag
     4261 cggagtcgtt actacttacc ccgctttttt ctagaaattc taaaatttct ttttctgatc
     4321 tttctctgac tttgatctta tttaaaatct tagaatataa tttttcatcc aaatatttag
     4381 attaacgttt agagaattgt gttccacgtc tagctttatg tagaccatat ttcttacgtt
     4441 ctaccattct agaatctcta gttaggaacc cttccttctt taagatactt ctagactcag
     4501 cacttttaag tgacaatgct cttgcaattg catgacgaat tgcttctact tgtcctgtaa
     4561 atccgccacc atgaacattt aactgtagat catatttatc tcttacattt aaaattgtaa
     4621 gcggagcaat cgctgcagca actttcgcct ctcccgtttt aatataatct ttgatgtctt
     4681 ttttgttaac tgtgattttt ccagttccat cagtaagttt taatctagct acagaagtct
     4741 ttcttcttcc tactgtccaa gtgcttttat cttttgccat ttaaatgttc cttaaaatta
     4801 tatttcgatc gctactggct tttgtgcctt tagattatgc tcgctacctg caaaaactct
     4861 taaattagaa agcattacat ctcctaattt tgacttcggt aacattccct taacaccttc
     4921 cattaaaact ctctcagggt ttgtttgaat taaatcctta aacgggatcg cactcattcc
     4981 acctggatgt aaagagtggt gatagtaaat tttttgatct ctttttctac cggttacttg
     5041 cacttttgcc gcattgatga tgatgatgtt gtctccacaa tcttggttgg gagtaaaagt
     5101 tgctttatgt ttgcctctaa gtctggctgc aacagcggaa caaagtcttc ctaatgtttt
     5161 tccttctgca ttgataacaa accagttttt cttaatttgt tcctttttta aagaaggtgt
     5221 tctatgtgct ggtgatatga ttggcataat gtaaaaataa tccttttatt gtcataattt
     5281 tgtatctata tttattggca aagttttttt attgaaactg ataaaaaaat gaatgttttt
     5341 atgggtgaaa gccattccct attctatatt tgtttcgata acagtactct gccgttttgc
     5401 ctgtatagcg aatctcatcg gcacaagtgt gacattcctt tttacatttt tttgtttgta
     5461 aaaggcaaaa tagaggaata tccgtagagc ttggaaaaag ggtcttatag tttataaggc
     5521 aatttaaatc agcagccttg cactgattgt ggacataaca ggcatctaaa ttttgctcat
     5581 aggaacaaga agataaaaaa agcaaaagaa ttactatata aattgatgta aaaaaaatct
     5641 tcttcaaact acaccgcata aattctgatc cataattatc aacgagcttt aaatttagta
     5701 tggatgcaaa ctattattct attgatcgtc taaagtggaa gattttttat ctagaagtat
     5761 tttactatag ttcgctttta ttatcttaat ctacattttt aataaatgac attacgcaaa
     5821 atgaaaaata ttttaaataa aagaatgaat tgaatagaat gtaaacttta tttgcctctc
     5881 tggcaatagt attacatagg ctcttgttaa ttagaattct ttctaggcta taataaaatg
     5941 aacgaagaaa aaattatacg acatctgtat aaaaatcaat cctttgatcc ttttgtggat
     6001 gattgctttt ggatggataa aaaatatcta gttacaacag acacgatgtg cgaaggaact
     6061 cattttaaac atgaatggag ttctcctaaa gatcttgctt gtaaattagt tacattaaat
     6121 gtttctgatc ttttttcttc cggtggaatt cccaaatatg catttttaaa tttaggttta
     6181 tctaaaatat cttccaattc taaatggctc agctccttca caaatgcact tcgaaagaat
     6241 ttacagaaat acaatataat tctatcagga ggtgacacct atcgctctca aactactaat
     6301 ctcaatttaa cactcattgg aactataaat catctgattc aaagaaaagg agcctctgta
     6361 ggtgataaaa tttttataac aggcaaaatt ggtctgtcta ttcttggatt aaaatatttg
     6421 gagaataata tagatttgcc gcttcgattt aaaaaagccg cattaaaaaa acacctggca
     6481 ccagaggtaa gaaatctctt tgctaaaaat ccaagcattt ataaaaatat gacagctatg
     6541 atggatatat cagatgggtt gattcaagat gcaaataaat tagcaaaggc ttctaacgtt
     6601 cacttaaata ttcatattga gaaaattcct ttttttgtta actacgaaaa atatctgact
     6661 atagatgatg ccctaaattc aggggaagaa gcggagtatc tttttacggg cgatgtgaat
     6721 ttgaaaaaat ctaaatttat aacattgatt ggcgaggtgc tttcagggaa tgcaggcgtg
     6781 agttttttga aagacaataa aaaatattct ccaaaagaaa aaggtttttt acatttttaa
     6841 ttttttgaat gaaggcgatt taaaaattta cgtaaaaatc aaagaaatct tcttctacta
     6901 catttcttct tctgttaaaa ttctgtttcc tttcttcgtc tcctttctcc catttggcaa
     6961 gtatcgcatt gatttctttt aattctgtct gtgtcatttt ttcaaattcc ctaaattcaa
     7021 tttctgcttc tttttttaaa taggctaatt taataggctt cagcaataga aatccccaag
     7081 atggttcttt ctttgcggct tttatggagt tagcaatgct ttttagtttt tgcattaatt
     7141 tagaatttgc agattttaaa tcaaaaacag aactctcaat tccgattgac cctactttta
     7201 tattgaacat agtaataata aaatctaatt tatctaaatc tttctgtttg tatgcttctt
     7261 gtgcctcatg ccatagctct ttttccaaat cagatgcgtc tcgattttta tctggatgta
     7321 atttttgaac tataatccta tatctttctt ttaaacgtga gtcgagatct ttctcgcttc
     7381 cctttctccc aagtccaaag ccttttcgta tagattcaaa atcttcagca ctctgattaa
     7441 atttttcatt gaatctttta aagaattcat ttagatcttc tctaaaaata tcctcttcat
     7501 cttcaaaatt attttcccaa aagttttcat tctcagttga ggttttttct ttttcctctt
     7561 tgggattgac attttttctt ttttctataa caattttata ggcttctaca aacgttatac
     7621 cctgtcgaaa tgcttcatct tgcacttctt ctattaattc tcttttttct tgaatttcaa
     7681 tagaggactg tcttatcttt tggatttttt ctccaaaatt ttcttggatc catctatcat
     7741 aagctggctt atctattttt tgaaattggt caatttctct ttgtgcttta tcaatttttt
     7801 taattgtttt ctgacattca gagataattt tttttctaat tactgtttgg ttaacagtga
     7861 ttagccgtct ttcatttgta ttaatctttg gagcagtttc cttagtgtaa acttttttct
     7921 tctttgcaga ataatacttt cggctgttag aagaagtttt tatcactgca ttgcctgctc
     7981 tagatctgct attaaatctt ctttcgattc tattccaacc gaaagacgca gtaatctatc
     8041 tgttaaacca attttatcta aaatatcctt tgggattgct gcatgagttt gaacaattgg
     8101 gtaagtgatt aggctttcta ctccacccag actttctgca aacgcaatga tttttactct
     8161 ttttaaaatt tgctctacta gtttttgttc ttttaattca aaagaaatca tagctccgaa
     8221 tccggtagat tgtttcttgg aagtttcatg tccagggtga gttttgattc ctggataata
     8281 gactttttca accaatggat gttgcaatag ccagttagca attgcatttg cattctcttc
     8341 ttgtctttgc attctaagac caagagtttt tagtccacgt aacattaacc aactatcaaa
     8401 aggagcaaga ccagaaccaa ccgtattttg tataaaacga attttttctc caagatttga
     8461 atctttcact gcaattaagc cagccaaggt atcattgtga ccacctatga attttgttgc
     8521 actacaaatc actatatcca ttccaagctc aagtggtctt tgaaaaaatg gagataaaaa
     8581 agtattatca aaaatagtta atatattttt ttcttttgcc agatcagcaa cagcctttat
     8641 gtccgtgatc ttcatcattg gatttgttgg agattcaata aatattgctt tcgtgttttt
     8701 gtttatttct ttttgaatat tttctacatt ggaagaatcg acatagacaa atttctgatt
     8761 tcgatttttg caaatctctt gaaaaaagcg atagctgcca ccatacaagt catcacatac
     8821 aataaattca tcccctgctt tgaataaatc aaatattgca gagatagcag ccataccagt
     8881 tgtaaatgca aacccaaatt taccactttc caaaatagca taagaatttt ctaacttttc
     8941 tcttgttggg ttttgtgttc ttgaataatc atagccagta gattttccta actcaggatg
     9001 ttcaaaggta gcagtctggt atataggcat actaatagag cctgttacgg atttatgtgc
     9061 atcaccacca tgaactaata ttgtatctat atgcatgtag ttgatatatt gatagttgat
     9121 ttagaagtca attgaaaaaa ggatatgcga agctgttaaa acagcttcgc tctttgaatg
     9181 aaagttgcgg ataacaaaga ttaggcgaaa agaccttcaa tagacaagta tctttcccct
     9241 gtatcataac aaaatgttaa aacttttgca cctgcaggta tttcatttat tttcttgcta
     9301 acagcagcaa gagatgctcc agaagataca cctacaaaga ttccttcttc tttagcagaa
     9361 cgaacggcat attcaaaagc ttcttctttt gtaatttgaa ttactccatc tagaagttta
     9421 gtatcaaggt tctttggaat aaaacctgca ccgattcctt gaattggatg tggacctggc
     9481 tttggaggtt cattatttaa cgttgctgtg attactgggg atgcagctgg ttcaacagca
     9541 aatactttta aatttggaaa tttttctttt aatacttttg caacaccagt gatatgacca
     9601 ccagttccaa caccagtaat taaataatct aatccatcag aaaaatcatt taagatttct
     9661 tttgctgttg tattaatgtg cacttgtatg tttgcatcat tttcaaattg ttgtggcatc
     9721 catgcatttt ttttagaagc tactaactct tttgcttttt caatcgcacc aggcataccc
     9781 ttttctcttg gtgttaattc aaaagtagca ccgtaggcag acatgagtct tcttctttcg
     9841 atactcattg attccggcat tacaagaatc aaaggatatc cttttaccgc tgcaaccatt
     9901 gcaagaccga ctccagtgtt accagaagtt ggctctatga taatgctatc tttctttaaa
     9961 atacctttct tttctgcatc ttctatcatc gatagagcaa ttctatcttt gatacttcca
    10021 cctggattcg ctttttctaa cttcacccac acatttgctt tatttccata aagtctgttt
    10081 aattttacat gtggagtgtt tcctattgtt tggagaatat tttctgcttt catgaattcc
    10141 tcttataatg caatttaatt gtattacact attttggagg aatgttttat agtcaatcca
    10201 ttattccaat aaattaaaca ttttgtatgt tttattagaa atttatcttt tatcagtgaa
    10261 agtcatttct ccaccggtta cgtttccatt tagaaattct cctgcaacag cagttccgtc
    10321 cggtaaagtg agtgttcctt ttccgttata agttccattt ttaaattcgc caacatactt
    10381 ttcacctgta tagctatcaa aggtgccttg gccatgaaaa agaccctctt tgaattctcc
    10441 aacataaaca gttccatttg gaaaagtaag agttccctta ccatcaaact tattattttt
    10501 gaagtcgcct acatattttt ttccattttt aaatgtaaga accccatgac cgctataatt
    10561 gccacttgca aatttaccta tgtatttaga accactttta aaatagagat ttccatcccc
    10621 gtcaaatttt ccttctttaa aacgcccttc gtatctttct cctgaattaa aagcgagtgt
    10681 tccatttccg ttaaatttac cttgcttaaa ctcaccatca tatttatcgc catttggaaa
    10741 agagagaact ccatctccat caaacattcc atttgcgaag ttgcctacat acttttcccc
    10801 atctgctaga attaatgttc cctcaccgtc aaag
//
LOCUS       DAOQOS010000157        10826 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_3157_length_10826_cov_6.080215, whole genome shotgun sequence.
ACCESSION   DAOQOS010000157 DAOQOS010000000
VERSION     DAOQOS010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 10826)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10826)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10826
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3157_length_10826_cov_6.080215"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1067
                     /locus_tag="PLF66_22800"
     CDS             <1..1067
                     /locus_tag="PLF66_22800"
                     /inference="COORDINATES: protein motif:HMM:NF019121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HMZ66935.1"
                     /translation="GRKLAKRQSLFTMTSRSVFLVADIYESFPELDSNLKQEWENQKS
                     IDYLKETILNLGYNCSILEPKKSKIDFLTSLQKLFADKSKIENCILFNLVEGYNSRNR
                     EAYIPALSEMLGIPFAGSDAYAQCISLDKFLTKQVVKSLNIQMKESYYIEDTSNIPVN
                     LHYPLFIKPNSEGSSLGIQSDNIVSSRQNLIHKLKQLLKEYSSVLMEPYLSGSDYTLG
                     IIGNYPNYKVTKVAEVKYPAIVYDSEIKTKDSMPEKLYFTMNSNLEAKIQRDSVEICK
                     RIKVNGYARIDWKSDEKGSPFFLEVNLTPGLSAFYSALPICYENSFGNYSDLIKNILQ
                     FGYEHYNTSKNFGYGRYEKN"
     gene            1159..1842
                     /locus_tag="PLF66_22805"
     CDS             1159..1842
                     /locus_tag="PLF66_22805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66936.1"
                     /translation="MEETELNRKSRENILKIGFNTLSEIEEKVKSFRIMNQNAAKRRY
                     IITRENIRDNAQKIIIPKAAEIDISATMLLRRQFKGDTIFKTFQPDEGIVIISDMATM
                     EGVTLTMDIVTQIMNLGGGAYEGFIDRVDSFTDFINHLKKALFPKLIIIGYIPKERIQ
                     SEMINFVRVKRIDNYLRTLEITHSVHKPVGYFPKIKQVPITEDPKSWGRFVIEIVREY
                     TKPYLIEQV"
     gene            complement(2155..4731)
                     /locus_tag="PLF66_22810"
     CDS             complement(2155..4731)
                     /locus_tag="PLF66_22810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002978250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HMZ66937.1"
                     /translation="MYNPFYIVQTLTKYMPIDTSKVNQKISVPSGQVIFEEGSPANSL
                     NIIHEGAFIIEKKINNSTIPILQLVGKNLTPGVISLFTSGRYPCTIKTAQDSIISTYQ
                     VNSSTIKKTIQGKVSIGIMTARTLLREIMEIFKKVNALQSLNSKVSKLNDNLSLSYFL
                     LEPTVFPGVDPTNYVYNPDLEMQTDPILRLTRENLSNFLERGGVLPESASIAFLNDDH
                     HELLKKEYLEEIDFDDEEFLFIRKILSVDPSINTPLFEADVTILIHMCEKLSLVYSSL
                     LEIMEEQVAFIVENMNLLCGRENSLLEKYNLMLEVMETGISDQKPEVLIPITESFHDQ
                     VGRYLLSYRNIFFKEFPMLSPNFSMFKQRSQTLQAGLKPQETDLLSDIDGNSNQPIRA
                     GLDMDAMKKELENSASKILNFVKLPAEQIKEFSSLILKLKTMSNPLDPESDARKIRKN
                     IAKTYWEVYDNCFQKYQANRSVPKQVEMMLNFGFFDETLLEPNQTAFIYTYKDDSLGR
                     REIPIHTGPEWLEMIFNKKITTSLDEMGQTYFDKIKMESKDVVYKKETDIPPNIDTNA
                     SRFKYEMSAMYQPNVRLTTGNPATHLPILTKYHITIPLEKCVVSKKTLSDTISSILQL
                     DYTAFNREIVYNDEQTGIRKEFVQRSIIPDFILVPSLGGKVMMWQDLSILRGNGSKES
                     RGRIILPIFANGDLKTMMLEAIAAFRWELCKNILGPDWNNPGIPSITSEYMDYIQFYK
                     KSKDLSIELKEKISTEFKRFRTDRDKFVNDYLSWIKYESEGIQRLNRVVRNIFYKHIP
                     FQKEIRDKISKLPAYADMHNRFTNIRNRQYREFENRYKKYASPDGSLPPVLQENLDFY
                     KV"
     gene            4787..5476
                     /locus_tag="PLF66_22815"
     CDS             4787..5476
                     /locus_tag="PLF66_22815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HMZ66938.1"
                     /translation="MNKTVTNAKEAIEGIADGMTLMVGGFGLCGIPEKSIQALLESGV
                     KDLTIISNNCGIDDFGLGLLLKNHQIKKMISSYVGENKTFEKQFLDGTLEVELNPQGT
                     LAERIRAGGAGIPGFFTPTGVGTLVADGKETREFNGRLYILETALTADYSIVKAWRGD
                     KAGNLIYRKTARNFNPMMATAGRITIAEVEELVEVGELDPDHIHTPGIYVQRIFQGEN
                     YEKRIERRTVI"
     gene            5476..6138
                     /locus_tag="PLF66_22820"
     CDS             5476..6138
                     /locus_tag="PLF66_22820"
                     /inference="COORDINATES: protein motif:HMM:TIGR02428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit B"
                     /protein_id="HMZ66939.1"
                     /translation="MPLTKEEQIKRVAEEIKEGYYVNLGIGMPTLVANYISPDLDIIL
                     QSENGLLGLGPFPLAGTEDADLINAGKQTVTALKGASYFSSADSFGMIRGGHIDLTIL
                     GAMEVSEEGDLANWMIPGKMVKGMGGAMDLVGGAKRVVVVMEHVSKEGKPKLLKKCSL
                     PLTGVKCVHMIVTELGVFEIEKGKLVLTERAEDVTVVDIRSKTEADFLVSNNLKIIKM
                     AN"
     gene            6157..7293
                     /locus_tag="PLF66_22825"
     CDS             6157..7293
                     /locus_tag="PLF66_22825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004490911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HMZ66940.1"
                     /translation="MNLKKLKKFKNARVGILTNQSAFTNGKYHFEILRENLDLKVIFI
                     PEHGLFAELQDQVSGSGLKYDFGNIKLENLYGDEESSLFVKSDTLKDLDLIVFDIRDV
                     GARYYTFLTSCYYILDAVGKYNISAKDHSIEILVIDSPNPAGKNVEGSPLLKKYSSFV
                     GVETVLHRHGLTIYELMEYYQNEFSISVKIHKIPIGEFYSKKDKPFLWVPPSPNIPAV
                     TTCYVYSGQCLLEGTNLSEGRGTTRPFETFGAPYIQIQDQRLLKRLTEYQTESFILRP
                     LLFIPTFHKHKGEICGGFQILLTQKKKFHSLLFSLHLLRTLREFYPNQFQFLKGVYEF
                     RSDYSAIELLAGDDKLLDFLNGESSYLEIKDYLTVCEENWKKKF"
     gene            complement(7383..8951)
                     /locus_tag="PLF66_22830"
     CDS             complement(7383..8951)
                     /locus_tag="PLF66_22830"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HMZ66941.1"
                     /translation="MANHFKQETYYKTIHSMNKIRMGLFLFYVASLSTAYKIFLKEQL
                     ITHFIAVFIFGFTAIFFYFRSKIRDFHPLIPFSLLVLDITLINFLNAYDATFGKIYAS
                     GIVKNPALFSLYFFAIFYSSFLLHKKFVLLCGFYSALGNLVLIFVAYQSGLSFSDDPN
                     LFNNVGYTSLSVEIVKCLFLISFSHMISTLMDILQTTIAKTNDSIQLTDQTNQTLESQ
                     KRDILETSKTLNTTMLEWSKKITSFLSDIQQQVEQIKKVNSFLEEFTISQEKIAKLSV
                     EQSKDSDELNDLSLNAEEKRILAIEKNQELAKNLDLIQENGHKIRNSLTENQQSGDKL
                     NQFFNKLSEVTSVISEISEKTNLLSLNASIEAARAGEAGKGFAVVASEVGKLADYSSE
                     NAKEISSIVKESRKVILDSDQTREKVSNSVENQFKQLDKISFIIQDITTFNNTLKKTN
                     ETYISSLKQFKSNADLLSERVKVNFQNTLEVKNSLGNISGKILYISDELRNMESDLKN
                     IQNVSNKLEDLASK"
     gene            complement(9125..9673)
                     /locus_tag="PLF66_22835"
     CDS             complement(9125..9673)
                     /locus_tag="PLF66_22835"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HMZ66942.1"
                     /translation="MNTTINAEITPDMKMGEILNIYPGAKRALFQKYHIGGCSSCGFS
                     MDETLEEVFINHRRDKEVQNAIQYIYHSAEVDRQMQISPKELKEKLDKGEDIKLIDVR
                     EDWEMEIATIPGSEMITQKLAYEILNKWDKNSQIVFICHHGLRSLEATSYFKGHGLPN
                     CKSLIGGVDLWSVEIDPSMKRY"
     gene            9999..10766
                     /locus_tag="PLF66_22840"
     CDS             9999..10766
                     /locus_tag="PLF66_22840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003003540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HMZ66943.1"
                     /translation="MKVVQYLLVGVLFFGALSVVGYFTIVTDGGPLKKEANSMKIYFP
                     NGEGIKIGNKVTVQGVPYGYVSRINLVQVDLEGNVLPKGEEGIGTRVEVIITIPANIK
                     MYENYEIKIKNESLLSGRVISIDPGTALERDPKTNEILPNAKKNKEINLSNQDNSNKL
                     KGKTNDDPLVSLSELIAENRNDIRKTISNIADISGKINSGKGTLGRLINQDDIHSNVN
                     TTLTDAQIVLRELREGLEDTREQAPVTSFIRAALSAF"
BASE COUNT         3660 a         1586 c         1937 g         3643 t
ORIGIN      
        1 ttgggagaaa gttagcaaag aggcaaagtc tgttcacaat gacaagtaga tcagtttttc
       61 ttgttgcgga tatctatgaa tcctttccag aattagattc taatttgaaa caagaatggg
      121 aaaatcaaaa aagtatagac tacttaaaag aaacaatttt aaatttaggt tataattgtt
      181 ctattcttga gccgaagaaa agtaaaattg attttctaac ttctcttcaa aaactattcg
      241 ccgataaatc taaaatagaa aattgtattt tatttaattt agtagaaggc tacaattcta
      301 gaaataggga agcttatatt cctgctcttt cggaaatgtt aggaattcca tttgcaggtt
      361 cggatgcata tgctcaatgc atttcacttg ataaattttt aaccaaacaa gttgtaaaaa
      421 gtttaaatat ccaaatgaaa gaatcctact atattgagga tacctctaat attccggtaa
      481 atctacatta tccgcttttt attaaaccaa atagcgaagg ttctagttta ggtatccaat
      541 ctgataatat agtcagttct agacagaatc tgattcataa attgaaacag ctcttaaaag
      601 agtattcttc cgttttaatg gaaccttatc tttctggttc tgattataca cttggtataa
      661 ttggaaatta tccaaattac aaagtaacga aggtggcaga agtaaaatac cctgccattg
      721 tgtatgattc ggaaattaaa acaaaggatt caatgcctga gaagttatat ttcacaatga
      781 attcaaacct tgaagctaag attcaaagag attcagtaga gatttgtaag agaataaagg
      841 taaatggata tgctagaatt gattggaaaa gtgatgaaaa ggggagtcca ttttttttag
      901 aagtgaatct aactccagga ttgtctgctt tttatagtgc tctgccaatc tgctatgaaa
      961 actcttttgg taattattca gatctaataa aaaatatatt gcaatttggc tacgagcatt
     1021 ataatacgtc aaaaaatttt ggctatggaa ggtatgaaaa aaattagata aattgaaaaa
     1081 ttacttttca atttaatcga aggtttcata gataatcaat acgagagttg aatacttaag
     1141 ccgatagaag aatatagaat ggaagaaaca gaactaaatc gaaaaagcag agagaatatc
     1201 ttaaagattg gctttaatac tttgagtgaa atcgaagaaa aagtgaaatc ttttcgaatt
     1261 atgaatcaaa atgcggctaa gagaagatat attattacaa gagaaaacat tcgcgacaac
     1321 gcgcaaaaga ttattatacc taaagcagcc gaaattgata tctctgcaac aatgctttta
     1381 agaagacaat ttaagggcga taccattttt aaaacatttc aaccagacga aggcattgtg
     1441 attatttctg atatggcaac tatggaaggt gtaacgttaa ccatggatat tgttacacag
     1501 attatgaatc taggaggtgg ggcatatgaa ggttttatag atcgtgtaga tagttttact
     1561 gatttcatca accatcttaa aaaagctctc tttccaaaac taattatcat tggttatatc
     1621 cctaaagaaa gaattcaaag tgaaatgatc aactttgttc gtgtaaagag aattgataat
     1681 taccttagaa ccttggaaat cactcatagt gttcataagc ctgttggata tttcccgaaa
     1741 atcaaacaag ttccaattac agaggatccg aaaagttggg gtcgttttgt aattgagatc
     1801 gttcgagaat atacaaagcc ttatttgatt gaacaagttt aaaaatttag tatctttaat
     1861 tccctgctca atgattcaaa ttctatactc acagaaatag attattttat ttaagcaaat
     1921 caaaacctat aatataaaaa tagttctaga gactgactag tcctttttaa aaaggataga
     1981 ttggtttacg aaatgatttt tccttgatga atcgaaatat tttagtcatt caaaaagcat
     2041 tggtaggttg gtagaagttc ctttgggaat gaaaatttaa ctcaccggaa tgatccatag
     2101 cttttttaga aatgaatcat ttgggtgagt ttttaatatt ttttttgtag ggatttacac
     2161 cttatagaaa tccaaatttt cctgtaatac aggtggtaaa cttccatctg ggcttgcata
     2221 tttcttataa cggttttcaa actctctata ctgtctattt cgaatatttg taaatcgatt
     2281 gtgcatatcc gcataagcag gaagctttga aattttatcg cgaatctctt tttgaaatgg
     2341 aatatgcttg taaaaaatat ttcgaacgac tcgattgagt ctttgaattc cttcagattc
     2401 atatttaatc caagaaagat aatcatttac aaacttatct ctatctgttc taaatcgctt
     2461 gaattctgtg gaaatctttt cttttaactc aatcgataaa tccttactct ttttatagaa
     2521 ctgtatatag tccatatatt cagaagtaat agaaggtata cctggattat tccaatcggg
     2581 tcctaatata ttcttacaaa gttcccatct gaatgctgcg atcgcttcta gcatcattgt
     2641 ctttaggtca ccatttgcaa aaatcggtag gataattctt ccacgacttt ccttggaacc
     2701 atttcctctt agaatagata agtcctgcca catcattact tttccaccta acgatgggac
     2761 taaaatgaaa tcaggtatga tacttctttg gacaaactct ttacggattc cggtctgctc
     2821 atcattgtat acaatttcac gattgaatgc agtgtagtct agttgtagaa tagaagaaat
     2881 tgtatcactc agtgtttttt tggacacaac gcatttttcc agtggaatcg taatatgata
     2941 cttggttaag ataggtaagt gcgttgcagg gttaccagtc gttaacctca cgttcggctg
     3001 atacattgca ctcatttcat atttaaatct actagcgtta gtatctatat tgggaggaat
     3061 atcagtttct tttttgtata ctacatcttt tgattccatt ttgattttat caaaataggt
     3121 ttgccccatt tcatctaggg aagtagtaat ttttttatta aagatcattt ccaaccattc
     3181 aggtccggta tgaattggga tttctctacg tcctagagag tcatctttat aagtataaat
     3241 aaatgctgtc tgatttggtt cgagaagagt ttcatcaaag aaaccaaaat tcaacatcat
     3301 ttctacttgc ttcggaacgc tacgatttgc ttgatatttc tgaaagcaat tatcataaac
     3361 ttcccagtaa gtctttgcaa tattctttct tatcttacgt gcatcacttt ctggatcgag
     3421 aggatttgac attgttttca attttaaaat aagagaggag aattctttta tttgttcagc
     3481 aggaagtttg acaaaattaa gaatcttact cgcagagttt tctaattctt tcttcatagc
     3541 atccatatca aggccagctc gaattggttg attggaattg ccatcaatgt ctgatagtaa
     3601 atccgtttcc tgtggtttta agcctgcttg caatgtttgt gagcgctgtt tgaacatgct
     3661 aaagttaggc gaaagcatag gaaattcttt aaagaatata tttcgatagg aaagtaaata
     3721 tctaccaact tggtcgtgaa aggattctgt gatgggaatt aaaacttccg gtttttgatc
     3781 actgataccc gtctccataa cttctaacat gagattatat ttttcaagca ggctattttc
     3841 tcgcccacag agtaaattca tattttctac aataaacgct acttgttctt ccataatttc
     3901 taatagggaa gaataaacta gagacagttt ctcacacata tgaatcaaaa tagttacatc
     3961 agcttcgaaa agtggagtat taatactagg atctactgaa agaatttttc ttataaatag
     4021 aaattcctca tcatcgaaat caatctcttc taaatattcc ttttttaata actcatgatg
     4081 gtcatcattt aaaaaagcga tactcgctga ttcaggaaga acaccacctc tttctaaaaa
     4141 gttacttaga ttttctcttg ttaatcgtaa aattggatct gtttgcattt ctagatccgg
     4201 attataaaca tagttggttg gatctactcc tggaaaaaca gttggttcta gaagaaaata
     4261 ggacaaggag agattatcat tcagctttga aacttttgaa ttgagtgatt gaagagcatt
     4321 tactttttta aaaatctcca taatctctct aagtaaagtt ctcgctgtca ttatacctat
     4381 tgatactttc ccttgaatcg tctttttgat tgtagagcta ttgacttgat acgtagagat
     4441 gattgaatct tgtgctgttt taattgtaca aggatatctg ccagatgtaa aaaggctgat
     4501 tacaccggga gtaaggtttt tgccaactaa ctgcaaaatt ggaatcgtag agttattaat
     4561 ctttttctcg attataaatg caccttcatg aattatattt aatgagttcg caggggaacc
     4621 ttcttcaaat ataacttgcc cggatggaac ggatattttt tgatttactt tacttgtatc
     4681 tattggcata tatttagtta gtgtctgaac aatataaaat ggattataca ttcataacaa
     4741 tcttttttta ttttcgatag attctaattt tataaggata aaatttttga ataagacagt
     4801 tacaaatgca aaagaagcga ttgagggaat tgcggatgga atgaccttga tggtgggcgg
     4861 gtttggttta tgtggaatcc ctgaaaaatc cattcaagct ttgcttgagt ccggtgtaaa
     4921 agatttaaca attatttcga ataattgtgg aattgacgat ttcggtttag gcctcttgtt
     4981 aaaaaatcat caaataaaaa agatgatctc gagttatgta ggagaaaata aaacatttga
     5041 aaaacaattt ttagatggaa cactcgaagt agaattaaat ccacagggaa cactcgcgga
     5101 aagaattaga gcaggcggtg ctggtatacc tggttttttt acaccgactg gggtaggaac
     5161 actagtagct gatgggaaag aaactaggga gtttaatgga cgattgtaca ttttagaaac
     5221 tgcactcact gcagactatt ccattgtaaa agcctggaga ggggacaagg ccgggaattt
     5281 aatctataga aagacagctc ggaatttcaa tcctatgatg gcaactgccg gtaggataac
     5341 gattgcagaa gtagaagaac tggtggaggt aggagagctg gatccggatc atattcatac
     5401 tcctggaatc tatgtgcaaa gaatttttca gggtgaaaat tacgaaaaga gaattgagag
     5461 aaggactgta atataatgcc attaaccaaa gaagaacaaa tcaagagagt agcagaagaa
     5521 attaaagaag gctactatgt taacctagga attggaatgc ctacactcgt agcgaattat
     5581 atctctcctg atttagatat tattttacaa tccgagaatg gtcttttagg actaggtcca
     5641 tttccgttag ccggaaccga agatgcagat ttaatcaatg ctggaaaaca aacagtaacc
     5701 gctctgaaag gagcttctta tttttcttca gcagattctt ttggaatgat tcgaggtgga
     5761 cacatagatc taacgattct tggagctatg gaagtttcgg aagaaggaga tttagctaat
     5821 tggatgatcc ctggtaaaat ggtaaaaggg atgggtgggg cgatggattt ggttggaggg
     5881 gctaaacggg ttgttgtagt aatggagcat gttagtaagg aagggaagcc aaaacttcta
     5941 aagaaatgct ctctcccttt aacaggtgtt aaatgtgtgc atatgattgt aacagaactt
     6001 ggagtttttg aaattgaaaa gggcaaattg gtattaaccg aaagagcaga ggatgttacg
     6061 gttgtggata tcagaagtaa aacagaagca gattttttag ttagtaataa tttaaaaatt
     6121 ataaaaatgg ctaattagta tttaccccat ttaaaaatga acttaaagaa attaaaaaaa
     6181 tttaaaaatg ctagagtagg aatcttaacc aatcaaagtg ctttcacaaa tggaaaatat
     6241 cactttgaaa ttcttcgaga aaatctagat ttaaaagtga tttttattcc agaacacggt
     6301 ctatttgctg aattacaaga tcaagtctca ggaagtggtc ttaaatacga tttcggaaat
     6361 ataaaactag aaaatctata tggagacgag gagagttctc tctttgttaa aagcgatact
     6421 ttaaaagatt tagatttaat agtatttgat atcagggatg taggagctag gtattataca
     6481 tttttaacca gctgttatta tattttagat gcggtaggta aatataacat atccgcaaaa
     6541 gatcattcga ttgagatatt ggttattgat tctcctaatc cagccgggaa aaatgtagag
     6601 gggagtccct tgctgaaaaa gtattcttcc tttgtgggag ttgaaacagt tttacacaga
     6661 catggactta ctatctatga attaatggaa tactatcaaa atgagttttc gatttcagtt
     6721 aaaatacata aaattccaat aggagaattt tattctaaaa aggataagcc atttttatgg
     6781 gtgcctccat ctcctaatat tccagcggtt actacttgtt atgtctattc tggacaatgt
     6841 ttattagaag gaacgaatct ttcggaaggc agaggaacga caaggccatt cgaaaccttt
     6901 ggtgctccct atattcaaat tcaagatcaa agacttttaa aacgtctgac ggaatatcaa
     6961 acggaaagtt ttattttgag acctttattg tttataccaa cttttcataa gcataaagga
     7021 gagatctgtg ggggatttca gattctactt acacaaaaaa agaaatttca tagtcttctt
     7081 ttttccttac atttacttag aacgttaaga gaattctatc ccaatcaatt tcagtttttg
     7141 aagggagttt atgaattccg atcagattac agcgccatag aactactagc aggcgatgat
     7201 aaattattag attttttaaa tggcgagtcc tcttatctgg aaataaaaga ttatttaacc
     7261 gtttgtgaag aaaattggaa aaagaaattc tgaagaaaaa taaatatgtt taatttaaaa
     7321 tcatataagt aaatgagaga ttctgatttt aataaatttt atttactatc caagaaaata
     7381 attcatttag atgctaaatc ttctaattta ttagaaacat tctgaatatt ctttaaatcg
     7441 gattccatat tcctcaattc atcagaaata tataagattt taccagaaat atttccgaga
     7501 gaatttttaa cttctaaagt attttgaaaa tttactttca ctctttctga aagtaaatct
     7561 gcatttgatt taaactgctt cagagatgag atatatgttt catttgtttt ctttaaagta
     7621 ttattaaaag ttgtgatatc ttgaataatg aaagatattt tatctaattg tttgaattga
     7681 ttttcgacac tattacttac tttttctctt gtttggtcag agtctagaat aacttttcta
     7741 ctttctttta cgatagatga aatctctttt gcattctcac tgctatagtc tgctagtttt
     7801 ccaacttcag atgcaaccac ggcaaaccct tttccagctt cgccggctct cgcagcttct
     7861 atagatgcgt ttaaagataa taaatttgtt ttttctgaaa tttctgatat gactgacgtt
     7921 acttctgata atttattaaa gaattggttt aatttatcac cagattgttg gttctctgta
     7981 agagaatttc gaatcttatg accattttct tggattaagt ctagattttt agcgagctct
     8041 tgattttttt caatggcgag tattcgtttt tcttctgcat ttaaagagag atcattcaat
     8101 tcatctgaat cttttgattg ttctacagaa agttttgcta ttttttcttg tgaaattgta
     8161 aattcttcta gaaatgaatt cactttttta atttgctcga cctgttgttg tatatcggac
     8221 aagaaggatg taattttttt actccattca agcattgtag tattgagagt tttggatgtt
     8281 tccaatatat ctcttttctg ggattctaaa gtttggtttg tttgatctgt taattggatc
     8341 gagtcatttg tttttgctat tgtggtttgt aatatatcca taagtgttga tatcatatgc
     8401 gaaaagctga ttaagaataa acattttact atttcaacgc ttaatgatgt gtagccaaca
     8461 ttattaaaaa gattaggatc atcagagaaa ctcagaccag attgataagc aacgaaaatt
     8521 agaactaggt taccgagagc ggaataaaaa ccacatagca atacaaattt tttatgaagt
     8581 aaaaaactag aatagaaaat tgcaaaaaag taaagtgaaa acaaggctgg attcttcaca
     8641 attccagaag catatatctt cccaaaagta gcatcgtaag cattcaaaaa attaatcaga
     8701 gttatatcta aaaccaatag actaaaggga ataagcggat gaaaatctct aatctttgat
     8761 ctaaaataaa aaaatatagc agtaaatcca aatataaaaa cagcaataaa gtgagtaatg
     8821 agctgttctt tcagaaaaat tttatacgca gtagaaagac tcgctacata gaataaaaaa
     8881 agacccatgc gtattttatt cattgaatga attgttttat aataggtttc ctgtttaaaa
     8941 tgattagcca taaaaaatct ctctagatga attaggatag gtaggctagc ttaatttttt
     9001 ttgagaaatt acaagtattt ttctgagttt tttagtgtga ggaggatatt aaatgttatg
     9061 tgaaagattt ggtgtgtaaa gatattagat attgcctcta tctttacaca tttcagagat
     9121 ttgattaata acgtttcatg ctaggatcaa tttctacaga ccataaatct acaccaccaa
     9181 tcaggctttt gcagtttggc aaaccatgac ctttaaaata ggatgttgct tctagacttc
     9241 taagaccatg gtggcatata aatacaattt gactattttt atcccattta ttcaatatct
     9301 cataagctag tttttgagta atcatttcgc tgccaggaat ggttgcaatt tccatttccc
     9361 aatcttctcg gacgtcaatg agtttaatgt cttcgccttt atctagcttt tcttttagct
     9421 ctttagggct aatttgcatt tgtctgtcca cttctgctga atgatagata tactgaattg
     9481 cattttgaac ttccttgtct cttctatgat tgatgaaaac ttcttctaaa gtctcatcca
     9541 tagaaaatcc acagctggaa cagccgccaa tgtggtattt ttgaaataga gctcgtttcg
     9601 ctcctgggta aatatttaaa atttcaccca ttttcatgtc gggtgtaatt tctgcgttta
     9661 ttgttgtatt catagttctt tcctttatgt tcaggtgaaa gcagaaatta attgtcaata
     9721 tacgtctcgc acttcttttg tgcaagttta attgttacag tttagacaat taaaaatggg
     9781 atttttaata atctttccgt aaaaaacata aaaagaccct gtattttgct tagagagaaa
     9841 catgtgaatt tgaatgggtt ttgctaggta tgaatcagaa tttaattcga atgataatcg
     9901 gaatattgac agtaataacc gaattaaaga accaaatcat tggttcaaat caataaaagt
     9961 attgtaagag aaggatttac ttatagggtg tgaattatat gaaagtggtg caatatttat
    10021 tagtaggcgt tttatttttc ggtgcattgt ctgttgttgg gtattttaca attgttacag
    10081 atgggggacc attaaagaaa gaagccaatt ctatgaagat atatttccca aatggagagg
    10141 gaattaagat aggaaataag gtaacagtgc aaggagttcc ttatggctat gtatctagaa
    10201 tcaatctagt tcaagttgat ctagaaggaa atgtcttacc caaaggagag gaggggatcg
    10261 gaactagggt agaagttatt attacaattc ctgctaacat aaaaatgtat gagaattacg
    10321 aaataaaaat taaaaacgaa agccttttgt ctggtcgtgt aatctctatt gatcctggaa
    10381 cagcacttga aagagatcct aagacgaacg agattttacc taacgcaaaa aagaataaag
    10441 aaattaatct ttcaaatcag gataattcta ataaactaaa aggcaagaca aatgatgatc
    10501 cattggtaag tttatcagaa ttaattgcag agaatagaaa cgatatacga aagactatat
    10561 ccaacattgc agatatttca ggaaaaataa attcaggaaa aggaacgtta ggcagattga
    10621 ttaatcaaga tgatatacat tccaatgtaa atacaacttt aacagatgca caaatagttt
    10681 tacgtgaatt acgagaaggt ttggaagaca caagagaaca ggctcctgta acaagcttta
    10741 ttagggctgc cctctccgct ttttagggta aaaaattttt atttttttgt taaaaaaaag
    10801 ggaaaaaaag tataaaaaga gtttga
//
LOCUS       DAOQOS010000158        10782 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_3178_length_10782_cov_5.880302, whole genome shotgun sequence.
ACCESSION   DAOQOS010000158 DAOQOS010000000
VERSION     DAOQOS010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 10782)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10782)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10782
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3178_length_10782_cov_5.880302"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1612
                     /gene="greA"
                     /locus_tag="PLF66_22845"
     CDS             <1..1612
                     /gene="greA"
                     /locus_tag="PLF66_22845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002176403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003711 - transcription elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HMZ66944.1"
                     /translation="DLFNNDIPNFFVELLTSHENIMTLGDIKAEITTKFLSKSDEWSK
                     WWNKAKVLLKKDPRIGFNPKKKDEIIYRQKPISLTEELSDKFAALTDPNKKLDIALEA
                     LEVYEEAEGAVESFNHFYYEEEESKDIFRKLVAYLYLEMASDIIHSDDLPRHQKPDDM
                     KRLIQDLSKEELLNFSKQLTNVEIKKNYLNLIRNYHPDYTNIFMGILFEVPVKVNKYA
                     FGILVGENKHSELNLFIETAINKSKENPEVFLWVAKSIISGSWEYPWLNISNQDLILR
                     TFRLLKPLAKIEEKGTKLKNAAMDILFGNDNEAISAIIKSSDSDFIRKIYALFKEVPY
                     ITDAEKDKLFNLINKLIPGFSWEDKFTSSEEETSDDSYFIPANVILVTRMGFNAKKEE
                     FEHLVNVEMAENSRDIGEAQEKGDLRENAEYKAAMERQVQLQAQIKKLEGELKSARIL
                     DITDVKTDKINIGCTVKLKNETTSEEIVYSILGAWDADTEKNIISYQSPMGKALLGKK
                     IGDTAVVDFEGSHMNFQVLEINRYTASI"
     gene            2160..2648
                     /locus_tag="PLF66_22850"
     CDS             2160..2648
                     /locus_tag="PLF66_22850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020771628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HMZ66945.1"
                     /translation="MILLIDGFNLIYKFPELEGLMYESKLNQAREGLLKILHQYKVKR
                     GNTVLHVFFDGKKDSGSEVRVDEYGGIKIYYSHDVTADFLIKEYIKRDPNPSNLYIVT
                     SDNDILFYCKRFNCRSQKSEDFAKWVEKFLFAPVDNEDKPENIKLSNKELEYWQDLFK
                     KR"
     gene            2662..3666
                     /locus_tag="PLF66_22855"
     CDS             2662..3666
                     /locus_tag="PLF66_22855"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HMZ66946.1"
                     /translation="MGSKKSIDRKDDFQRLLNVFKNGNDFILTTHKSCDPDGIGSELG
                     LSFLLTKLGKNHSILNPDKIPEKYKFIDPQLKVRHIDPNILQNFSTDKTVVVVDNSDL
                     PRIGDVNQYLKPDKSNLILVDHHDNIEPFPGLFSFPEIGSTSEIIFEIIELAGFQLDY
                     NTAIALYLGIVMDTGQFKYSKTRPRTHEIAAKLVQHNFPIEELLRKMYEDFPTSVLLL
                     KKDIYSSIEIHPDLHLITVEITKQMLGKYNFHSNPIEGITSELLGPSDIFVSVGFTEG
                     EDDFIKISFRSKGNYDVCSVAKDFNGGGHKNASGAMVKGSMKKVKEDVMHRLLSLLKT
                     "
     gene            3676..5163
                     /locus_tag="PLF66_22860"
     CDS             3676..5163
                     /locus_tag="PLF66_22860"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001971527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HMZ66947.1"
                     /translation="MLFNTIPFFIFFTIVYLIYWNIGKKFRIEFLILSGSIFYYYYSP
                     LFLIHFLAVICINYLLYYKIYSGNKNLYVKLAVIFNLLNLGFFKYFYFFTGVLSDITG
                     NEFLKSASEDRWIHITLPLAVSFYSFQMIACAVDTYRATEEEELLSFKKYLLFILFFP
                     VLIAGPIMRKKDFFPNLEREVPKQDDIYRACYLMMSGLIKKILMADPIATIVNPIFAN
                     PSEYNAFSLFMVGILSVIQIYADFSGLTDLARSVAYFLGFEIPENFFAPFFSKSGREF
                     WKRWHVTLSSWLMDYIYIPLGGSRAGEARTYLNLIITMTLGGIWHGADYTFLVWGFYW
                     GVLLAIERYLEKVKGINLTPEKNPFLIVLKVTIIYILVSISLLMFRSINTTSMIALFQ
                     GLITNSPDYLSSLLRTNNGEWLIDGMELVQSEKPFLLNSIKSYESVFYTYVAFLFFHF
                     IQYKPESLDRFKKYNPWLVILLGVITIFLLTTLSHDGDSFIYTTF"
     gene            5166..6257
                     /locus_tag="PLF66_22865"
     CDS             5166..6257
                     /locus_tag="PLF66_22865"
                     /inference="COORDINATES: protein motif:HMM:NF019243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1574 domain-containing protein"
                     /protein_id="HMZ66948.1"
                     /translation="MIKNKFLLIPVIIFILALAIDRILMLEKIQTYFTKTVSEINYFH
                     KPILFDELQEYLKKPDRKKVLVYFGNSRGLLFDNKYIETHYPDWILFNFSVPGGTPDY
                     FTFWLEQFQEKNVKPDFILVDDAVEAFNFTARIKIDEVLVNGLDFPFIIKYRNRYTSK
                     EITNFIAKKLFKTYQYRPKLETVLQRMKNDAVIVKAYRDWREKIAKKLKEERGSASSD
                     ISANATSNADLVQKYSEGDFHSYIEPFTFNENMLKFQKDNFQILQKMNVKHAAIWVRV
                     ARPYFSFIQNRNAILPAKDEPITAYSIWYQKLSDAHKEYNVPLFNMNEDPKYNCDFFT
                     DASHMSTQCFPDYTDYIFQNIIGIKKKSN"
     gene            complement(6271..7023)
                     /locus_tag="PLF66_22870"
     CDS             complement(6271..7023)
                     /locus_tag="PLF66_22870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004786259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HMZ66949.1"
                     /translation="MYVKDYCFTGLKGGTETEDMDYDELKLLSLLGRDLVPVIVKIGG
                     PEARTDIRFCKANGIDGISAPMIESQYALKNFISTLKNLIPPAQYESVHKSMNLETIT
                     GYRNILEIADSKSFEELNNVTAARSDLSASMDLSPDDPEVMRVTTQIVKIAKDRGKKT
                     SVGGTITKANFFMIAEMAKPEQINSRHVIVSVNDVIEKNLTDVAEVMLSFEMDLFALL
                     GQLKPEKAYYYNNRIELNRERIGKRKVLYSIR"
     gene            7397..7591
                     /locus_tag="PLF66_22875"
     CDS             7397..7591
                     /locus_tag="PLF66_22875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010575608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66950.1"
                     /translation="MLYEDEFDDEYDAEDDEYDDTTVTMACDDCDYRWKISLEVDDEE
                     ESYDEDLICPMCGSSNVAEI"
     gene            7967..8713
                     /locus_tag="PLF66_22880"
     CDS             7967..8713
                     /locus_tag="PLF66_22880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66951.1"
                     /translation="MKLIIIFILQILVLFIQPIFSETEENPTLKRILELYKQKDFKEI
                     SKVASEYIRTKEDISQDDKVIKFYFHTENDLKKLDILLDKIYKDTEELNSKFHSLVYM
                     LMDKALLAEEYEIGVKWGDVYRKEAKSKKAKYFRGLYLYSCLLYKANKSNLSLSIIDS
                     ALKDKPNTKIVSKLKLLRIAQLKNDFQIIVESQNYLKNNQETIYADFIFAHMVQAYRN
                     AGKKKRVEQLKEEFKKDFGNSILVDTVMGI"
     gene            8963..9036
                     /locus_tag="PLF66_22885"
     tRNA            8963..9036
                     /locus_tag="PLF66_22885"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8997..8999,aa:Val,seq:gac)
     gene            complement(9105..10109)
                     /locus_tag="PLF66_22890"
     CDS             complement(9105..10109)
                     /locus_tag="PLF66_22890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66952.1"
                     /translation="MLKNIFLIIALVVSSSLFAIESFVYHEGSSDKFFVEQVRLINNG
                     TLQEKIAAIETLKKSKTKRALRPLILALKGVTQNAEPTKSQTQAISEKDNYAPINFVI
                     PDNNRPVIKFLAAQALAEIGNELAIKPLADQYKILAEQVTKDKDQKIFYADTEKMPAV
                     VAAGEILMSLGYLLDPFEDKESFDVIVKALDHEHFFVRACAAEALRNTRREAAIAAID
                     GAIGKEKDDYARTVMLASVVGIKKTNSKHFFQLLDMLKNPSPLVRIKTSSMLGELAIA
                     SSETYLRQAMMIEDDLLVRNQMKKDIGYITGYQIPNAPSSTYNTSSDERDRNKQSAAP
                     "
     gene            complement(10375..>10782)
                     /locus_tag="PLF66_22895"
     CDS             complement(10375..>10782)
                     /locus_tag="PLF66_22895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66953.1"
                     /translation="QSVKPSDDRFYQIDIILRGTALREKNKWNIREIRKLEKFLESAD
                     DFELHRYFGLNEEIYLGMRAKKAIQSIKRDAKLDENWRKKNDPIFEYYLTHQATSEER
                     LFYKKNKGLFFQPETGYALIQFLKQQVEFKKTK"
BASE COUNT         3809 a         1655 c         1715 g         3603 t
ORIGIN      
        1 agatttattt aataatgata ttccaaactt ctttgtagaa ctcttaacat ctcatgagaa
       61 tattatgacc ttaggagata ttaaagcaga aattacaacg aagtttcttt ctaaatcgga
      121 tgaatggtct aaatggtgga acaaagcaaa agttttactc aaaaaagatc caagaatcgg
      181 ttttaaccct aagaaaaaag atgaaattat ctacagacaa aaaccaattt cccttacaga
      241 agaactttct gataaattcg cagctttaac agatccaaat aaaaaactag atattgctct
      301 agaagcattg gaagtatacg aagaagcaga aggtgctgtt gaatctttca atcacttcta
      361 ttatgaagaa gaagaaagca aagacatttt ccgtaaatta gtagcttatc tttatctaga
      421 aatggcttct gatatcattc attcagatga cttaccacgt catcaaaaac cagatgatat
      481 gaaaagatta attcaagatc tttctaagga agaattatta aatttctcta agcagctaac
      541 taacgttgaa ataaagaaaa actatttaaa tctcatccga aattatcacc cagattatac
      601 aaatatattc atgggaattc tttttgaggt tcctgttaaa gtaaataaat atgcttttgg
      661 aattttagtc ggagaaaata aacactctga gttaaatcta tttatcgaaa cagcaatcaa
      721 caagtccaag gaaaatccag aagtcttcct ttgggtagca aaatcgatta tatccgggtc
      781 ttgggaatat ccttggttaa atatttccaa tcaagattta atccttagaa cattccgcct
      841 attgaagcct cttgcaaaga tcgaagaaaa aggaactaaa ctgaaaaatg ctgcaatgga
      901 catcctcttt ggaaatgaca atgaggctat ttctgcaatt attaaatcta gcgattctga
      961 tttcatccga aaaatctatg cgctctttaa agaagtgcca tatataacgg atgcagagaa
     1021 agataaactc ttcaatctga ttaacaagct cattccagga ttctcttggg aagataaatt
     1081 tacaagctca gaggaagaaa catcagatga cagttacttc attcctgcaa atgttatatt
     1141 agtgactaga atgggattca atgcaaagaa agaagaattt gagcatttag taaacgttga
     1201 gatggcggag aactctagag atatcgggga agctcaagaa aaaggggacc ttagagaaaa
     1261 tgctgagtat aaagcagcaa tggaaagaca agttcaattg caagctcaga ttaagaaatt
     1321 agaaggtgaa ctaaagagtg ctcgaattct agatattacg gatgtaaaaa ctgataaaat
     1381 taacattggt tgcactgtta aattaaaaaa tgaaacaact tcggaagaaa ttgtttattc
     1441 tatccttggt gcatgggatg ctgatactga gaaaaatatc ataagctacc aatccccaat
     1501 gggtaaagcc ttattaggca aaaaaatcgg agatacagcg gttgtcgact tcgaaggaag
     1561 ccatatgaat ttccaagttt tggaaattaa cagatataca gcttctatct aaaccaagat
     1621 aaacaaggga ttgtgtagag tcagaaagtc aacttcattg aacattgact tttttacaca
     1681 atccctactc tctcctcttt tcaaatcatt taaataagtt tacaaagctc caaaatagaa
     1741 acatctcaaa gaatcaatag gtttactctg catggatcaa tagattcaga ccaaaatcga
     1801 aaaggattgc ctagaagctt ttgatcctac ttgctctttg aatcagtcca gcttatacca
     1861 aatgagaaaa gaaacttcca caaggatttt ctttcagttg catttaaaat accgctcagc
     1921 gagacatggg aacactaaga agtgtttaga gtgaatactt atttgtgttt caaaaatggg
     1981 aatttaattt tgacgttcct tagaatacac aaaccaagat cagtatccgt aaaataaaat
     2041 aggtaaaaca gggtttcaaa ataggaaata tttcacttct tttatcacaa cagagtataa
     2101 aaaaatctaa aacgaataat ctcttgccat tttatccttg gattttatga tatcaaacta
     2161 tgattcttct aatcgatgga tttaatttga tttacaaatt tccagaatta gaaggactca
     2221 tgtatgaaag caaattaaat caagcaagag aaggtttact taaaatatta catcaataca
     2281 aagtaaaacg gggcaataca gtattacacg ttttctttga tggtaaaaaa gactcaggaa
     2341 gtgaagtcag agttgatgaa tatggaggaa taaaaattta ttatagtcat gatgtaacag
     2401 cagatttttt aataaaagaa tatataaaaa gagatccaaa tccctctaac ttatatatag
     2461 taacatcaga caatgatata ttattttact gtaaacgctt taattgtaga agccaaaaat
     2521 cagaagactt tgcaaaatgg gtcgagaagt ttttattcgc tccagtggat aatgaggata
     2581 agccagagaa tatcaaattg agtaacaaag aattagaata ctggcaagat ttatttaaaa
     2641 aaagataaag agagaaatgg tttgggaagt aaaaaaagta tagatagaaa agatgatttt
     2701 caaagacttt taaatgtttt taagaatgga aatgatttta tattaacaac tcataaaagc
     2761 tgtgatcctg atggaattgg atcagaacta ggtttatctt ttttattaac aaaattaggt
     2821 aagaatcatt ctattttaaa tccagataag attcctgaaa aatacaaatt tatagatcca
     2881 cagcttaagg taagacatat agatccaaat attttacaaa atttctcaac agataagaca
     2941 gttgttgttg tagataattc ggatttgcct cgaattgggg atgtcaatca atatctaaaa
     3001 ccagataaat cgaatttaat tttagtcgat caccatgata atatagaacc ttttcctggt
     3061 ttattttcat tcccagaaat tggttcaaca tccgaaatta tttttgaaat aattgagtta
     3121 gctggttttc aattggacta taatacggct atcgcattgt atttaggaat cgtcatggac
     3181 acaggtcagt ttaaatacag taaaactcga cctagaactc atgaaattgc agcaaagctt
     3241 gttcaacata acttcccaat agaagaattg ctcagaaaaa tgtatgagga ttttcctacc
     3301 tctgttcttt tattaaagaa agatatctac tctagtatag aaattcatcc tgacctacat
     3361 ttaattactg ttgagattac aaaacaaatg cttggaaaat ataatttcca ttcaaatccg
     3421 attgaaggaa ttacaagtga gctattaggt ccaagtgata ttttcgtatc tgttggattc
     3481 acggaaggcg aagatgattt tataaaaatt agttttcgtt ccaaagggaa ttatgatgtt
     3541 tgtagtgttg caaaggattt taacggtggc ggacacaaga acgcaagtgg tgcgatggta
     3601 aaaggatcta tgaaaaaagt aaaagaagat gtgatgcacc gtttattatc tcttttaaaa
     3661 acttagtgaa gaaacatgtt atttaatacg atcccttttt ttatattttt tacaattgtc
     3721 tatttaatct attggaatat aggaaagaaa tttaggatag aattcttaat tctatctggt
     3781 tccatttttt actattacta ttctccatta ttcctaattc actttctagc tgtcatctgt
     3841 ataaattatc ttttatatta taaaatttat tcaggaaata aaaatttata cgttaagctt
     3901 gcagtgatat ttaatttact caatttagga ttctttaaat atttttattt ttttacaggt
     3961 gtactaagtg atataaccgg gaatgagttt ttgaaatcag catcggaaga tagatggatt
     4021 catatcacat tgcctctagc tgttagtttt tattcgtttc aaatgattgc gtgcgcagtg
     4081 gatacctatc gtgcaacgga agaggaggaa cttctaagtt ttaaaaagta tcttctattt
     4141 atcttatttt ttcctgtttt aatcgcagga cctatcatga gaaaaaaaga tttcttccca
     4201 aacttagaaa gggaagttcc caaacaagat gacatctaca gagcttgcta tctaatgatg
     4261 tctggtctta taaagaaaat tttaatggcg gatccaattg ctacgatagt caatccaatc
     4321 tttgcgaacc caagtgaata caatgcattc tctttgttta tggttggaat tttatctgtg
     4381 attcagatct atgcagactt ttcaggctta acagatttag ctagatcggt tgcctatttc
     4441 ttaggatttg agataccgga aaactttttt gctccattct tttctaaaag tggtagagag
     4501 ttttggaaga gatggcacgt aacgctctca agctggttaa tggattacat ttacattcca
     4561 ctcggaggca gtagagctgg cgaagcaagg acgtatttaa atctaatcat tacaatgacg
     4621 ttaggtggaa tttggcatgg agcagactat acatttctag tttggggttt ttattggggc
     4681 gtattgctcg caatagagcg ttatctggaa aaggtaaaag gaatcaatct gacccctgag
     4741 aaaaatccgt tcctgattgt tttaaaagta actatcatat atattcttgt atcaataagc
     4801 ctactcatgt ttagatctat caatacaact tctatgatag ctttatttca aggacttatt
     4861 actaattcac cagattatct aagtagcctc ttaagaacaa acaatggaga gtggttgatt
     4921 gacggaatgg aattagtcca aagtgaaaaa ccttttttat taaactcaat caaaagttat
     4981 gaaagtgtgt tctatacata tgtagcattt ttattttttc attttattca atataaaccg
     5041 gaatccctgg atcgatttaa aaaatataat ccatggttag ttatacttct tggagtaata
     5101 acaatatttt tactaacgac tctatcccat gatggtgata gttttattta tacaaccttt
     5161 taactatgat taagaataaa tttttactta taccagttat catttttata ttagcattgg
     5221 caattgatag aattctaatg cttgaaaaaa tccaaaccta ttttacaaaa acagtttctg
     5281 aaataaatta ctttcataaa ccaattttgt ttgatgaatt gcaagagtat ctaaagaagc
     5341 cagatagaaa aaaagtttta gtttactttg gaaattcacg aggtctactt tttgataata
     5401 agtatataga aacacattat cctgattgga ttctttttaa tttttctgtt ccagggggaa
     5461 ctcctgatta ttttacattt tggttagagc agttccaaga aaaaaatgtg aaacctgatt
     5521 ttattttagt ggatgatgcc gtggaagctt ttaacttcac agctcgaatt aaaatagatg
     5581 aagtcctagt aaatggatta gattttccat tcataattaa atatcgaaat cgttatacct
     5641 caaaagaaat aacaaatttt attgctaaga aattatttaa aacatatcag tatagaccta
     5701 aacttgaaac agttcttcaa agaatgaaaa atgatgcggt tattgtaaaa gcttacagag
     5761 actggcgtga aaaaattgca aaaaaattaa aagaggaaag aggaagtgca tcttctgata
     5821 tatcagctaa cgcaacttcc aatgcagatt tagtgcagaa atattcagag ggagatttcc
     5881 actcctatat tgaacctttt acatttaatg aaaacatgtt aaaattccag aaagataatt
     5941 tccagattct acaaaaaatg aatgtaaagc atgcggctat atgggtcaga gtcgctcgcc
     6001 cctacttcag ctttattcaa aatagaaatg caattttgcc ggctaaagac gaaccgatca
     6061 cagcctattc catttggtat caaaaactat cagatgctca taaagaatac aatgttccat
     6121 tatttaatat gaatgaagat cccaaataca attgtgactt tttcacagat gcaagtcata
     6181 tgtcaaccca gtgttttcct gattacactg attatatttt tcagaatatt attggaatca
     6241 aaaagaaatc gaattagtat tcgagcaatc ttatcgaata gaataaagaa ctttgcgttt
     6301 accaatacgc tctcgattta attctatcct gttattgtag taataagctt tttctggttt
     6361 taactgcccc aagagtgcaa ataaatccat ctcaaaagag agcatcactt cagctacatc
     6421 ggttaaattc ttctcaatta catcatttac acttacaatg acatgacgag agttgatttg
     6481 ctctggcttt gccatttcag ctatcataaa aaaatttgct ttcgtgatag tgccgcctac
     6541 agaagttttc ttccctctat cttttgcaat tttaacaatt tgtgtggtca ctctcataac
     6601 ctcaggatca tcaggactta aatccataga tgcagaaaga tctgatcttg ctgcagtaac
     6661 attgtttaat tcttcaaaag attttgaatc agcaatttct agaatattgc gatatcctgt
     6721 aatcgtttct aaattcatag atttatgaac agattcatat tgtgcaggtg gaattaaatt
     6781 ttttaatgtg ctaataaaat ttttaagtgc atattgagac tcgatcattg gtgctgagat
     6841 accatcaatc ccatttgctt tacaaaaacg aatatccgtt cttgcttcag gtccacctat
     6901 ttttacaatg acaggaacca agtctctacc aagtaaggac aataacttta attcatcata
     6961 gtccatgtct tctgtttcag ttcctccttt caaaccagta aaacaataat cttttacata
     7021 cattccaaga tctttcttta gattaaaaag ggaggtttct acagatttat tcatatttta
     7081 cctatcgaca taattctaaa atacttttct actatttgtc atttactttc tagcatattt
     7141 gaaaaataaa atttcgaaaa tattagaatt tgctaatgtt gtctagaatg aaagaaacct
     7201 aaactaaggt gcttttattt atataacaat gagcaatatt ttttcttact tttagagaag
     7261 cttttgatta tttcagagtt gcatttagtc taaatttcca gaaattattg aatgagaata
     7321 aatgttttga aataaatatt gacgaagaaa cattttttta aattcagttt agaaacttag
     7381 taaatggaag aaacttatgc tatacgaaga tgaatttgat gacgaatacg atgcagaaga
     7441 tgatgaatat gatgatacta cagttacaat ggcttgtgac gattgtgatt atcgatggaa
     7501 aatttcttta gaagtagacg atgaagaaga gtcttatgat gaagatttga tttgccctat
     7561 gtgcggttct tcaaatgttg cagagattta aagtatcgat gccttcatac actgccattg
     7621 catacattta ggatatcaat gaaagtactg ctgtttttca aaaaaatata tgaacaacag
     7681 cagtacttat aaaaaataaa ttgattataa aaatattcaa tagaatcaga taaaatttac
     7741 aacctcttta aaaaaattta tttatagaac gttaaactta aagcttgtca aaataaaacc
     7801 agaaagaaat cttagtcttt gtatctttgc acacaactgt taactttaat tcaattaaac
     7861 tttcttatat attttttctc agagaaattt ataattagtt ttaaaagtta aattgaaaac
     7921 agttcgagat ttgaaaccca aataaattag gaaaaaactg gaacaaatga aattaataat
     7981 tatcttcatt cttcaaattt tagttttatt tatacagcca atattttcag aaacagagga
     8041 aaatcctaca ttaaaaagaa tcttagagtt atataaacaa aaagatttca aagaaattag
     8101 caaggtggcg agtgaataca ttcgaacaaa agaggatata tcccaggatg ataaagtaat
     8161 taaattttat tttcatacag agaatgactt aaagaaatta gatatccttc tagataaaat
     8221 ttataaagat acagaagaat taaatagtaa atttcattct ttagtttata tgctaatgga
     8281 taaagcattg ttagcagaag aatatgagat tggcgttaaa tggggtgacg tctatcgcaa
     8341 ggaagcaaaa tcaaaaaaag caaaatactt tcgaggactc tacctatact cttgcttact
     8401 atataaagca aataaaagta atctttcctt atcaattatt gattctgctc taaaggataa
     8461 accaaataca aaaattgtaa gtaagttaaa acttttaagg atagcacagt taaaaaatga
     8521 ctttcaaatc atcgttgaat ctcaaaatta tttaaagaat aaccaagaaa caatatatgc
     8581 cgacttcatt tttgctcata tggtgcaggc atacagaaat gcaggtaaga agaagagagt
     8641 cgagcaacta aaagaggaat tcaaaaaaga cttcgggaat tccatattag tagatactgt
     8701 tatgggaata taaataattt tataaaatct tccttttcat ttatagttat tctgatctag
     8761 aatgttttct atcagaaaat aattccgcac cagaaaaaca atctgcaatc cagaattttc
     8821 gaattgtttt ttcataagag aatccaatta attcaacgct cttaattata cacaaatctt
     8881 cctgtgaaac atagattaag ttgatttgct aaatttttga ttgtaaaaag aagggtttta
     8941 aaaaaagtaa aaatatccga atggggaatt agctcagttg gctagagcgc ttccttgaca
     9001 tggaagaggc cactggttca aatccagtat ttcccattca gaataaacct cccgagaaat
     9061 actgttttct atatctaagc ctttaatttt ctattgctat aaaattaagg agcagcactt
     9121 tgcttgtttc tatctctctc atcgcttgaa gtattgtaag ttgaagatgg agcgtttggt
     9181 atttgataac cagtaatgta tccaatgtct tttttcattt gatttctaac taacaaatca
     9241 tcttcaatca tcatagcttg tcttaaatat gtttctgaac tagcaattgc aagttctcct
     9301 aacatactag acgttttaat tctaaccaat ggagatggat tttttaacat atctaaaagt
     9361 tgaaaaaaat gctttgaatt tgtttttttg attccaacaa cagatgctaa catgacggtt
     9421 ctagcataat catctttctc tttgccaata gcgccatcaa tcgcagctat cgcagcttct
     9481 cttcttgtat tgcgtagtgc ttctgcagca caagcacgaa caaaaaaatg ttcatggtct
     9541 aatgctttta caattacatc aaaggattct ttatcttcaa aaggatctaa taaatatcca
     9601 agagacatta agatttcacc tgcagcaaca accgctggca ttttttcagt atcagcatag
     9661 aaaattttct gatctttgtc ttttgtgact tgctctgcaa gaattttata ctgatcagct
     9721 aaaggcttta tagctaactc atttcctatt tcagccaatg cttgtgcagc taaaaattta
     9781 ataactggtc tgttattgtc aggaatcacg aagttgattg gtgcataatt gtctttctca
     9841 ctaatcgcct gtgtctgaga cttagttggc tctgcatttt gtgtcactcc tttcaaggca
     9901 aggataaggg gacgcaatgc tcttttggtt ttggattttt ttagggtttc gatcgcagca
     9961 atcttttctt gtaaagttcc attgttgatg agcctaactt gctctacgaa gaacttatcg
    10021 ctactacctt catggtaaac aaaggattcg atagcgaaga ggctggatga tactactagt
    10081 gcaataatta aaaatatgtt ttttaacatt ctttactcct atctctatat atcgtatata
    10141 aaaaaatctg tcttaaattt ctatctcttt ttcattttat agaaatccca aagatttatt
    10201 taatttgcgc acaatctaag tttttactga actttaaaga taaattctat ttctgggata
    10261 tgtatttggg attagttcat tcagaggctt tttatttttt atataaatat tttttataaa
    10321 cattttctta gatcaaaatc aagttcttat aacctatcaa tgatagcatc gatgttattt
    10381 agttttttta aactcaacct gttgttttag aaactgaatc aaagcataac ctgtttcagg
    10441 ctgaaaaaaa agacctttat ttttcttata aaaaagtcgt tcttcagaag tcgcttggtg
    10501 cgttagataa tactcaaaaa tagggtcatt tttttttcgc caattttcat ccaattttgc
    10561 atctctttta atactttgaa ttgccttttt ggctctcata cctaaataaa tctcttcatt
    10621 tagtccgaaa taacggtgta attcaaagtc atccgcactt tctaagaatt tttctaactt
    10681 tcgaatttct ctgatattcc atttgttttt ctctctaaga gcagttcctc ttagaatgat
    10741 atcgatttga taaaaacgat catccgatgg tttaacggat tg
//
LOCUS       DAOQOS010000159        10661 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_3230_length_10661_cov_7.031586, whole genome shotgun sequence.
ACCESSION   DAOQOS010000159 DAOQOS010000000
VERSION     DAOQOS010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 10661)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10661)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10661
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3230_length_10661_cov_7.031586"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..687
                     /gene="arsM"
                     /locus_tag="PLF66_22900"
     CDS             <1..687
                     /gene="arsM"
                     /locus_tag="PLF66_22900"
                     /inference="COORDINATES: protein motif:HMM:NF008823.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HMZ66954.1"
                     /translation="YNKEADLGLGCGIPTQFANIQEGNIVIDLGSGAGNDAFVVRSIV
                     GEKGKVLGIDMTEAMIEKARMNNENLGFNNVEFRLGEIEKIPSTSNVADVVVSNCVLN
                     LVPDKAKAFSEIHRVLKNGGHFCISDIVLAGNIPDILRKHADMYAGCVSSAIQKTEYL
                     AHIKNAGFANIEIKKEKVLTIPDDILQEYLTKEELELFKNSKTEIQSITVYAEKHSKK
                     SCCEPNSGCC"
     gene            887..3010
                     /gene="fusA"
                     /locus_tag="PLF66_22905"
     CDS             887..3010
                     /gene="fusA"
                     /locus_tag="PLF66_22905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015677895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HMZ66955.1"
                     /translation="MSTKTLSEKLKNTRNIGISAHIDSGKTTLTERILFYTNRIHAIH
                     DVRGKDGVGAKMDSMELERERGITIQSAATYCTWKNKNTNTDTTINIIDTPGHVDFTI
                     EVERSLRVLDGAILVLCGVSGVQSQSITVDRQMRRYSVPRVAFINKLDRTGANPFRVI
                     DQLREKLKHNAVAVQLPIGLEQNFTGVVDLVKMKAVYFEGNNGMEVVEREIPADMIEL
                     ANKKREELLDAVSLFSDELTEAMLEGTPTEEQIRKAIRQGVLELKLTPVFMGSAYKNK
                     GVQKLLDGVSDYLANPTEVTNKALDLNNNEAEIILESDPTKPLACLAFKLEDGRYGQL
                     TYVRIYQGKLSKGMTIYNSSTNKKHSVGRLVRMHSDEMEEIQSAEAGDIVALFGIDCA
                     SGDTFTDGSWKVAMESMFVPAPVISLTIECKESKQLPNLAKALNRFTKEDPTFKTEID
                     KESGQTIIKGMGELHLEVYVERMKREYGVDLVTGAPQVAYRETITKKAEFDYTHKKQT
                     GGQGQFARVAGYLEPIALEEGKNYEFVDKIVGGAIPREYISSCDKGFKSCLERGALIG
                     FPIVGVRAVITDGSYHDVDSSDMAFQIGARYGFRQGFNKANPIILEPIMKVEVDGPVE
                     FQGAILGGLNQRRGMIVATSEQDAYCKIEAEVPLADMFGYSTVLRSSTQGKAEFSMEF
                     SRYAPVPRNISEELLKKYKVKQDED"
     gene            complement(3104..3862)
                     /locus_tag="PLF66_22910"
     CDS             complement(3104..3862)
                     /locus_tag="PLF66_22910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66956.1"
                     /translation="MIKWKLMMTTLPYMVVVIAINYLLSNLLQFEGIIDFADVGLVLT
                     GGIFLIGFMLAGTMSDYKESEKLPSEIACTLEALEETIDAVCASKDLNGKNMKAILFE
                     ITSDIYNWFYKKISYEQMFSSLSRLDSVISQMEKVGASSYATRMLGELNSLRKIITRI
                     GVIARTSFLSTGYALLEVLITAIIILLLISKFKSVLAEYILIGFVSLIYIYMYRLIQD
                     IDDPFEYSETGKPGASEVPLFPIEEYKERAKARL"
     gene            3998..4798
                     /locus_tag="PLF66_22915"
     CDS             3998..4798
                     /locus_tag="PLF66_22915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66957.1"
                     /translation="MKFINAISILFLSSCSSLFWRDARLLPTLSFSENGKGIAYIQTH
                     YQEKDSWNPLNGTSLKKNYTTQISLADIQNDLSLKKNTDLERFSFWILPQSLFYNEAR
                     GILAFIQGLDQNEYATPNKTVSIHNIKTKITTDLLKGKSEIKTPLAIALSSDASQIAI
                     VSGEVNANGFYEKFQLFILDTMNQSIQASFSLPDWQDSPEYELGWLKSEATLSARVKS
                     KLFTLKGKSISESNFSGSNLADNPKYAYARVYKWESKDLELERIKLEK"
     gene            4982..5218
                     /locus_tag="PLF66_22920"
     CDS             4982..5218
                     /locus_tag="PLF66_22920"
                     /inference="COORDINATES: protein motif:HMM:NF016167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HMZ66958.1"
                     /translation="MIESIITIDPEIVSGTPVFQNTRVPTQSLFDYISTGESIDSYLE
                     DFPYVSRMQVNKVLKIAGELLMRTAQTIAHENTN"
     gene            complement(5223..6170)
                     /locus_tag="PLF66_22925"
     CDS             complement(5223..6170)
                     /locus_tag="PLF66_22925"
                     /inference="COORDINATES: protein motif:HMM:NF039905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HMZ66959.1"
                     /translation="MKKISAVLVFNPKSGKGKARERAIDFSEKWKEFTGTDLKLRPTA
                     SLADIRVAANETYSDGTIQIFMGGDGTLSESIQGISEKLNFKGLVQPVGFLPGGTGNS
                     FLRDFDIKDYKTARDRLLQSIDKNSTHSIDSAIITYKRVKSDLSVGEETKRIMFNIWG
                     VGLISDITKLAIRMRKIGSLNYTLASLIKLLSHRPYELKTIIDGIEEKLHCNMITVSN
                     SQYTGGKMQIAPHVRVNDGQLFLISPMLKSRIKLFQNFPKIFKGEHVNHPEIKTEFIK
                     NLEIHHDMPLIMNVDGELEEGYNPKMQIHPSYFKIYMIE"
     gene            6564..6932
                     /locus_tag="PLF66_22930"
     CDS             6564..6932
                     /locus_tag="PLF66_22930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002747711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HMZ66960.1"
                     /translation="MRISVGSLPQSLNEEALKKLFAEFGTVEEVVIKRDKKTQTSLGY
                     GHVDMPDDAGKKAIEKLNGKELEDKKIVVVDASQLHNDGKDKNYEKPVANSKIHGSKF
                     TGGGFSGSGVRKSGGGGRGK"
     gene            6936..8114
                     /locus_tag="PLF66_22935"
     CDS             6936..8114
                     /locus_tag="PLF66_22935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004786536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HMZ66961.1"
                     /translation="MATAKLLNTTIGNGKKFFLQFGGQGSPYLKEVSKLYKEEPLLKD
                     FFEVAFKTLNQLEKEVGRSDILISEGLDLKSWIENPDSAPSEDYQIRGSVSVAMIFIT
                     QVANYHLLTLKGFPVEELTAHTAGVTGHSQGIIGGALAAVAKSGNDFYKAFEDFLKFA
                     FYLGYRAQEMYPIFEVEKAVLDGNAEVGDKNPAPMVAVIGYSKEELEARVDAANKDLS
                     LSGQDKLSISLYNTPDSMIISAKPSSLLAFRKKYKAEMDERKAKFVYLRTTAPFHCPY
                     MEGSWEKFEVDLKTKVHFPYNVSDLKLPLHSIFDGRKIGASENLAEVLFKEIVIKALH
                     WDKAVSALFTDSSIATVIDFGPSAVTSKLTGGQLSAKNLTTQVLCLSNNKDLKVIFEA
                     "
     gene            complement(8277..9275)
                     /locus_tag="PLF66_22940"
     CDS             complement(8277..9275)
                     /locus_tag="PLF66_22940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008396489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine kinase"
                     /protein_id="HMZ66962.1"
                     /translation="MKKKYDVFGVGNALVDTLAAIDHDFLHKAEIRKGVMTLVDTESQ
                     NKLLTFLKDHKLELRSGGSAANTMIALANSGGNGCYTGKVADDEHGRFYKKDMEDAGI
                     VFDVPPAKEGKTGTCVVLTTPDADRTMLTNLGISTSLSKHDIQEERLKDSQIAYIEGY
                     LWDGTNTKEASMHTMELAKKYGNKVAFTYSDPFCVNRSREDFVKLTQEFVDIAFCNHD
                     EAMAMTETDNPEDAIMQLGTMCNLVFMTWGSRGAFIAVDGIVSPVTGYPVTSPVDSNG
                     AGDAFAAGVLYGLTHGYSISKACKWGNYVASKMIMEIGARLSISLKDHHHVLSGMA"
     gene            complement(9327..9965)
                     /locus_tag="PLF66_22945"
     CDS             complement(9327..9965)
                     /locus_tag="PLF66_22945"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - CoA pyrophosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HMZ66963.1"
                     /translation="MNLSFEDLKVNLANSSFPIVDSQGLKVSSVVMPFYRITDDQIGI
                     LLTKRSEHLKSHAGQISFPGGKYDETDGTLLETALREWEEETGANRNSLEIIGSYESM
                     STGTGFHITPFVALYSGDFTFNVNPREVDFMIKMDLEKLNQFPFYTVHWDAHPSGRIH
                     IYYIDLPEGLLWGATCHILYRFLRDYAGFDRKPILVKPNLNHPPFFQPPKKA"
     gene            complement(9962..>10661)
                     /locus_tag="PLF66_22950"
     CDS             complement(9962..>10661)
                     /locus_tag="PLF66_22950"
                     /inference="COORDINATES: protein motif:HMM:NF012779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HRDC domain-containing protein"
                     /protein_id="HMZ66964.1"
                     /translation="NEVHWHKFPNIDKFTPEQRRNIFDILHFRDDKARRMNKAPFRIL
                     NNETIEKIVKKEMPEENFINILGKKDGAELSRILQNPSGAPLDKADFPKPDYDLKPEE
                     ETPFKQLRKWRDKIMKKRNMDHTMLLSNKNLIQIIRSNVSTLEELRNLSLISEWKIQN
                     YGPSILKALKNEPYDDLINPLVPLLSSRKPKKPQPHQKQKTKDQKKEEKTEQKAESEI
                     ATREETVLEEPKET"
BASE COUNT         3430 a         1844 c         1980 g         3407 t
ORIGIN      
        1 tataacaaag aagcagattt agggcttggg tgtggaattc cgacacaatt tgcaaacatt
       61 caagaaggga atatagtaat tgatcttgga tctggagcag gcaatgacgc atttgtggtt
      121 cgatcgattg taggagagaa aggaaaagtt ctaggaatcg atatgacaga agctatgata
      181 gagaaagcta gaatgaacaa tgagaatcta ggctttaata atgtagaatt tagacttggg
      241 gaaattgaaa aaattccatc tacttccaat gtagctgatg tcgttgtcag taattgtgtt
      301 ttaaatttag ttccagataa ggcaaaagcg ttttcagaga tacatcgggt tttaaaaaat
      361 ggaggtcatt tttgtatttc agatatcgtt ttagctggaa acatacctga tattcttaga
      421 aaacatgcag atatgtatgc tggatgcgtc tcttccgcaa tccaaaaaac tgaatattta
      481 gctcatatca aaaatgcagg gttcgcaaac attgaaatca aaaaggaaaa ggttttaaca
      541 attccagatg acattttaca agagtatctt acaaaagagg aactggaact gtttaaaaac
      601 tctaaaacag agattcagag cattacagtc tatgcagaaa aacacagcaa aaagagctgt
      661 tgtgagccaa attctggttg ttgttgagtt ctttctcttg ctagtcaaaa aatacagaga
      721 aatcagagtt tctctgtatt ttagatcaat acgacccgat ccttgttgat tggctaacac
      781 ctacctgcct ttccatgcct tgttttttat tcgacctata gcctagcttt gcaaatatga
      841 catacaattg ggcaatttgc ccctacaatt ggaatagagg aaaatcatga gcacaaaaac
      901 tttgagcgag aaattgaaaa ataccagaaa tattggtata tcagctcaca tcgattcagg
      961 aaaaactaca ttaacagaac gtattctttt ttatacgaat agaattcacg ctattcatga
     1021 cgtccgtggt aaagatggcg ttggagcaaa aatggatagt atggagttgg aaagagagcg
     1081 tggtatcaca attcaatctg ctgctaccta ctgcacttgg aaaaataaaa atacaaatac
     1141 ggacactact atcaacatca ttgatactcc gggtcacgta gattttacaa ttgaagtgga
     1201 aagatctctt cgcgtattag atggagctat cttagttctt tgtggtgtat ccggtgttca
     1261 atctcaatct atcactgtgg atagacaaat gagacgttat tcggttcctc gtgttgcttt
     1321 tatcaacaaa ttagatagaa caggtgcgaa tcctttccgc gtcattgatc aacttagaga
     1381 aaaattaaag cacaatgcag ttgctgttca actaccaatc ggactcgaac aaaactttac
     1441 tggtgttgtc gacttagtaa aaatgaaggc agtttatttc gaaggcaata atggtatgga
     1501 agttgtggaa agagaaattc ctgctgatat gattgaactt gctaataaaa aaagggaaga
     1561 acttctagat gctgtttctt tattcagtga tgaattaaca gaagcaatgc tcgaaggaac
     1621 tccgacagaa gagcaaatca gaaaagcaat tcgtcaaggg gttctagagt taaaacttac
     1681 acctgtattt atgggttctg cttacaaaaa taaaggagtt caaaaacttc tagatggagt
     1741 ttctgactat cttgcaaacc caacagaagt tacaaacaaa gctcttgatt taaataacaa
     1801 tgaagctgaa attattttag aatcagatcc aactaaacca ctcgcttgtc ttgcatttaa
     1861 attggaagac ggacgttatg gtcagttgac ttatgttcgt atctatcaag gaaaactttc
     1921 taaaggtatg accatttata attcttctac aaacaagaag catagcgttg gacgtttggt
     1981 tcgtatgcac tcggatgaaa tggaagagat tcaatctgcg gaagcgggag atatcgttgc
     2041 gttattcggt attgattgtg cttcagggga tacgtttact gacggatctt ggaaagtggc
     2101 aatggaatct atgttcgttc ctgctcctgt gatttctctt acaattgaat gtaaagaatc
     2161 aaaacaactt ccaaaccttg cgaaagcgtt aaatcgtttt acaaaagaag atcctacatt
     2221 caaaactgaa attgataaag aatctggaca aaccatcatc aaagggatgg gagaattaca
     2281 cctcgaagta tacgtagaac gtatgaaaag agagtatggt gtagatttag taacaggtgc
     2341 tccacaggtt gcttaccgtg aaacaatcac taagaaagct gaatttgatt atactcataa
     2401 aaagcaaacc ggtggtcaag gtcagttcgc tcgtgtggct ggttatttag agcctatcgc
     2461 actcgaagaa gggaagaatt acgagttcgt tgataagatt gtgggtggag caattccaag
     2521 agaatacatc agttcctgcg ataaaggatt taagagctgt ctcgagagag gtgctctcat
     2581 tggattccca attgtaggag taagagctgt gattactgac ggttcttatc acgatgtgga
     2641 ttcatccgat atggctttcc aaattggtgc tcgttatgga tttagacaag gctttaacaa
     2701 ggcaaatcct atcattcttg aaccaattat gaaagtagaa gtagatggac cggtagaatt
     2761 ccaaggagca atcctcggtg gtttaaacca aagacgtggt atgatcgtag ctacatccga
     2821 gcaagatgct tattgtaaaa ttgaagcaga agttccgtta gccgacatgt ttggttattc
     2881 aactgttctt agatcttcta cacagggaaa agcagaattc tcaatggaat tttctcgcta
     2941 tgctcctgtt ccaagaaata tctcagaaga attgcttaag aaatacaaag ttaagcaaga
     3001 cgaggattag aatatttatc cattttcgca agtctgaatt gagcttgcga aaatctgtct
     3061 ttcttcaaaa aaagccaagt gtaaaaactt ggctttttta tttttacaat cttgcctttg
     3121 ctctttcttt gtattcctca attgggaaga gaggaacttc tgatgcaccc ggttttcctg
     3181 tttcagaata ttcaaaagga tcatcaatgt cttgaatcaa tcgatacatg taaatataaa
     3241 tcaagcttac aaatccaatc agaatgtatt cggctaaaac acttttgaat tttgaaatta
     3301 agagtaaaat gataatagct gttattaaaa cttctaacag agcatatcct gtcgatagaa
     3361 aactagttct tgcgatgact ccaattcttg taatgatttt ccgaagagaa ttcaattctc
     3421 ccagcatcct agttgcatag ctgctagccc ccactttttc catttgggaa ataacagaat
     3481 ctaatctaga aagggaagaa aacatttgtt cataggaaat ttttttgtaa aaccaattat
     3541 aaatatcaga agtaatttca aacaaaattg ccttcatgtt tttgccattt aaatctttac
     3601 ttgcgcaaac tgcatctatt gtttcttcta acgcttctaa tgtgcaggca atttcagaag
     3661 gaagcttttc gctttcttta tagtcagaca ttgttccagc taacataaaa ccaattaaga
     3721 aaattccacc agttaatacc agtcctacat ctgcaaaatc aataattcct tcaaattgca
     3781 ataaattact caagagataa ttaatcgcga ttactacaac catatatggt agggttgtca
     3841 tcatgagttt ccatttaatc attcgtaacc tctacaaatt ctttttcggg aatgctatgc
     3901 ttaaatgttt ttgtgtcaat tgctaaatga aaaaaggaat gaataatttt aagggagaga
     3961 ataaggttga taaaataatt ctaaatagga atctacaatg aaatttatta atgcaatctc
     4021 tattctattt ttatcatctt gttcaagtct attttggagg gatgcaagac ttttacctac
     4081 tctaagtttt tccgagaatg gaaaaggaat tgcatatatt caaacacatt atcaggagaa
     4141 agattcttgg aatccactca atggaactag tcttaaaaaa aactacacca ctcaaatttc
     4201 ccttgctgat attcaaaacg atttgagcct caaaaaaaat actgacctag agagatttag
     4261 tttttggatt ctaccgcagt ctctttttta taacgaggct agaggtatac ttgcatttat
     4321 tcagggacta gaccagaatg aatatgcaac tcccaataaa actgtatcta ttcacaatat
     4381 aaaaactaaa atcacaactg atttactgaa aggaaaatca gaaataaaaa cacctctcgc
     4441 aattgccctt tcttcggatg ccagtcaaat tgctatcgtg agtggtgaag taaatgcaaa
     4501 tggattttat gaaaaatttc agctctttat tttggataca atgaatcaat cgattcaagc
     4561 aagcttttct cttccagatt ggcaggattc tcctgaatac gaacttggtt ggcttaaatc
     4621 agaagcaacg ttatccgcgc gagtgaaatc aaaactattc actttaaaag ggaaatcgat
     4681 ttccgaatct aatttttctg gatccaatct agcggataat cctaagtatg cgtatgcgag
     4741 agtttacaaa tgggaaagta aggatttaga attagagaga attaaactag agaaatagta
     4801 gatattagga aaaagctttc atttcattgt tcaaatagca atcatattgc aatttttaag
     4861 agtttgacaa aaaataatat agtgtaattc tatttggagt aactgtaaat atttttcttt
     4921 tgcgctttta aggaaaagtt ttttctttta ttataatcat ttttagtaag taggggataa
     4981 tatgatagag tccataataa caattgatcc agaaattgta agtggaacac ccgtttttca
     5041 aaatactcgt gttccaactc agagtttatt cgattatatt tctactggtg aatcgataga
     5101 ttcttatcta gaggattttc cgtatgtaag tcgaatgcaa gtgaataaag ttctgaaaat
     5161 agcaggcgaa cttctaatga gaacagccca aactattgca catgaaaata caaattgatg
     5221 ggttattcta tcatataaat tttaaaatac gatggatgaa tttgcatttt aggattatac
     5281 ccttcttcta attctccatc tacattcata ataagtggca tgtcatgatg aatctctaag
     5341 tttttgataa actctgtttt gatttctgga tgattgacat gctctccttt gaagatttta
     5401 ggaaagtttt gaaatagttt gattctactt tttagcatcg gagaaattaa gaacaattgt
     5461 ccatcattga cgcgaacatg tggggctatt tgcattttac cgccagtgta ttgtgaattc
     5521 gaaacagtaa tcatattgca atggagtttt tcttcaattc catcgataat tgttttcaat
     5581 tcataaggtc tatgacttaa gagttttata agacttgcga gtgtataatt caaagatcca
     5641 atctttctca ttcgaatggc aagctttgta atatcagaaa ttaaaccaac tccccaaata
     5701 ttaaacatga ttctcttggt ttcttctcca acggacaggt ctgatttgac tctcttgtat
     5761 gtgatgattg cagaatcaat gctgtgagta gaatttttat caatggattg taagagtcta
     5821 tctcgtgctg ttttataatc tttaatatcg aagtctctca gaaaactatt tcctgttcct
     5881 ccaggaagaa atccgaccgg ttgcacaaga cctttaaaat ttagcttttc tgaaattcct
     5941 tgaatagact ccgagagagt tccatctcca cccatgaaaa tttggattgt gccatctgaa
     6001 taggtttcgt tagccgcaac acgtatgtca gcaagagaag cagtaggtcg aagttttaaa
     6061 tcggttccag taaattcttt ccatttttca gaaaagtcta ttgctctctc tcttgctttt
     6121 ccttttccac tctttggatt gaaaactaaa actgcactta tctttttcat gtgacctcta
     6181 cggatttcta ataaattcaa atggattttc caaaggtaga ataaaaaaag agtctgtcaa
     6241 ggttgttttc taaaatcgaa tccgatttat gaatctaaat agctaagtta ggcaaatctc
     6301 ctttgaacct tcgggggact gtgtagaagc aagattttca ccatgaagcg cacgaaggcc
     6361 acgaagaaac attgtaaatc tcctaggaaa ttttgacctt cgcgatcttt gtggttagat
     6421 tttttttaaa cttttacgta gtccctaaat cagaaaaaaa ggcttttcaa attactcgga
     6481 tctttttacc cttattcttg tattttaaaa attcataaaa tgcgaatttt taaaataatt
     6541 aaaaaaagaa aggaactata tctatgagaa tatcagtagg aagtcttcca caatctttaa
     6601 acgaagaggc tttaaaaaaa ttgttcgcag aattcggaac tgttgaagag gtcgtaatca
     6661 agcgagataa gaaaacacaa acctcacttg gctatggtca tgtcgatatg ccggatgatg
     6721 caggtaaaaa agcaatcgag aagttaaacg gtaaggaact agaggataag aaaattgtcg
     6781 ttgtagatgc ctcccaattg cataacgatg gaaaagacaa aaattatgaa aaaccagtcg
     6841 ctaacagtaa aattcatgga tcaaaattca ctggtggcgg attttcgggc agtggtgtcc
     6901 gtaaatctgg cggtggtgga agaggaaagt aaagaatggc aacagctaaa ttattaaata
     6961 caacaatcgg aaatggaaaa aagttttttt tacaatttgg tgggcaaggc tctccttatt
     7021 taaaagaagt ttctaagtta tacaaagaag aacctctttt aaaagatttt tttgaagtag
     7081 cttttaaaac attgaatcaa ctcgaaaaag aagtagggcg ttcagatatt ttaatttcag
     7141 aaggtctcga tctcaaatct tggatcgaaa atccggactc tgctccatcg gaagactatc
     7201 agatccgcgg tagcgtgtct gttgcaatga tttttataac acaagtggct aattatcatt
     7261 tgctcacact caaaggtttt ccagttgagg aactaacggc tcatacagct ggagttacag
     7321 gtcatagcca aggtatcatt ggtggagcac ttgccgctgt tgcaaaaagt ggaaatgatt
     7381 tctacaaagc ttttgaagac tttttaaaat ttgcttttta tcttggatat agagcacagg
     7441 aaatgtatcc aatctttgaa gtagaaaaag cagttctaga tggaaatgca gaagtaggtg
     7501 ataaaaatcc agctcctatg gttgctgtca ttggttattc caaagaagaa ttagaagcaa
     7561 gagtagatgc ggctaacaag gatttatccc tttccggtca agacaaactt agcatcagtc
     7621 tttataacac tcctgattct atgattatat ctgcaaaacc ttcttcttta cttgcattta
     7681 gaaaaaaata caaagcagag atggatgaaa gaaaagcaaa gtttgtatat cttagaacaa
     7741 ctgctccttt ccattgtcca tacatggaag gatcttggga aaagtttgaa gtggatctaa
     7801 aaacaaaggt tcatttccca tataacgttt ctgatctcaa acttccactc cattctattt
     7861 ttgatggaag aaaaattggt gcatctgaaa acttagcaga agtattattt aaagaaattg
     7921 taatcaaggc actccattgg gataaagcag tcagtgctct ctttacagat tctagtattg
     7981 caactgtaat tgattttgga ccaagtgcag ttacatccaa attaactggt ggtcaactct
     8041 ctgctaaaaa tctcactaca caagttcttt gtctttctaa caacaaagac ttaaaagtga
     8101 tcttcgaagc ataagatatt ttcagggtgg tgtaaaaatt ttttcaccac catgagcacc
     8161 gataactgaa cgatgaataa ttttgaatta tcgtatcttt tatcggtgaa tcgtccctcc
     8221 acccaaaatc cattaccgct cattcgcggt aataggtggt aaatttttaa aaaggtttac
     8281 gccattccag aaagaacatg gtgatgatct ttcaaagaaa tagaaagtct agcaccgatt
     8341 tccataatca tcttggaagc aacatagttt ccccatttac aagctttcga aatagaataa
     8401 ccgtgcgtta gtccatataa aacacctgcc gcaaaagcat cgcctgctcc attggagtct
     8461 acaggacttg taacaggata gcctgttaca ggagaaacga tcccatctac agcaatgaaa
     8521 gctccacgcg agccccaagt cataaaaact aagttgcaca tagttccgag ttgcatgata
     8581 gcatcttctg ggttgtctgt ttctgtcata gccattgctt catcgtggtt acaaaaagcg
     8641 atatctacaa attcctgagt cagttttaca aaatcttctc tagagcgatt cacacaaaat
     8701 ggatcgctat aagtaaaggc tactttgttt ccatatttct ttgctagctc cattgtgtgc
     8761 atggatgctt ccttagtatt ggttccatcc caaagatatc cttcgatata agcgatttgt
     8821 gaatctttga gtctttcttc ttgaatgtcg tgtttgctga gactcgttga aattccaaga
     8881 tttgtgagca tggttctatc tgcatctgga gttgtaagga caacacatgt tccagtctta
     8941 ccttcttttg caggaggaac gtcaaacaca ataccagcat cttccatgtc ttttttataa
     9001 aatctgccat gttcgtcatc ggcaactttt ccggtataac agccattacc ccccgagttt
     9061 gcaagcgcaa tcatcgtgtt agccgcactt cctccagatc gtaactctaa tttgtgatct
     9121 tttaaaaaag tgagcagttt attttgactc tctgtgtcca ccagagtcat tacacctttt
     9181 ctaatttctg ctttatgcaa aaaatcatgg tcaatcgctg ctagtgtatc aactagagca
     9241 tttcctacac cgaatacatc atactttttt ttcattcttt acttcctaca aaaactgtaa
     9301 ttaggctaag agatgaagta aataaatcaa gcctttttcg gcggctgaaa gaagggaggg
     9361 tgatttaggt ttggttttac tagaattggt tttctgtcaa aacctgcata gtcccgaagg
     9421 aaacgataca atatgtgaca agtcgcaccc cagagaagtc cttcaggtaa atcgatgtaa
     9481 taaatatgaa ttcttccgga tggatgcgca tcccaatgaa ctgtataaaa tggaaattga
     9541 tttaattttt ccaaatccat tttaatcata aaatctactt cgcgtggatt tacattaaat
     9601 gtaaaatcac ctgaatagag tgcaacaaaa ggagtgatat gaaatccagt tcccgtgctc
     9661 atagattcat atgagccaat gatttctagg gaatttctgt tagcaccagt ttcctcttcc
     9721 cattccctta aggctgtctc tagaagtgtt ccatcggttt catcatattt tccacctgga
     9781 aaagaaattt gtcctgcatg actttttaaa tgttcacttc tttttgtaag tagaattcct
     9841 atttgatcat ccgttatgcg atagaaaggc ataacaactg aggagacttt taatccctgc
     9901 gaatcaacaa tcggaaagct tgaattggca agatttactt tgagatcttc aaaggaaagg
     9961 ttcaagtctc ttttggttcc tccaagacag tctcttctct cgttgctatt tcggattctg
    10021 ctttttgttc cgttttctct tccttctttt ggtcctttgt tttttgtttt tgatgaggct
    10081 gtggtttttt aggttttcta gatgagagta atggaactaa cggattaatt aaatcatcat
    10141 aaggttcatt ctttaaggct ttcagaatag aaggtccgta gttttgaatt ttccattctg
    10201 atatgagaga tagatttcgt aattcttcta aggtagaaac gttggagcgt ataatttgaa
    10261 ttagattctt attagaaagt aacatggtat gatccatatt tctttttttc ataatcttat
    10321 cgcgccattt gcgaagttgt ttgaatggtg tttcctcttc tggttttaaa tcataatcag
    10381 gttttggaaa gtcagcttta tcgagcggag ctcccgatgg attttgtaag atacgagaaa
    10441 gttctgcacc gtcttttttt ccgaggatgt ttatgaaatt ctcttcaggc atttcttttt
    10501 ttacaatttt ttctattgtt tcattattta aaatgcggaa gggagcttta ttcattcttc
    10561 gagctttgtc atctctaaag tgtaaaatat caaaaatatt tcttctctgt tcaggagtga
    10621 atttgtctat atttggaaat ttatgccaat gcacttcatt t
//
LOCUS       DAOQOS010000160        10570 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_3277_length_10570_cov_5.387732, whole genome shotgun sequence.
ACCESSION   DAOQOS010000160 DAOQOS010000000
VERSION     DAOQOS010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 10570)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10570)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10570
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3277_length_10570_cov_5.387732"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..367
                     /locus_tag="PLF66_22955"
     CDS             <1..367
                     /locus_tag="PLF66_22955"
                     /inference="COORDINATES: protein motif:HMM:NF019224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1554 domain-containing protein"
                     /protein_id="HMZ66965.1"
                     /translation="CTLPNCPGGNGEHIGWIFQANKNYYQADGITLIGTTDANGLMPP
                     TFVNPATGNAKYWTGINVDWTTAVANNCVNWTSNAGGIYGNCGGSGSGPNTSPGNLLN
                     SWGGTVACNAPQQLLCIEQ"
     gene            complement(422..1465)
                     /locus_tag="PLF66_22960"
     CDS             complement(422..1465)
                     /locus_tag="PLF66_22960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010679700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HMZ66966.1"
                     /translation="MKAGVLYSNASHLKIEDVTLPELQPNQVKVDIKCCGVCGSDIHM
                     TIHKQVRLKHYPTILGHEASGVVSEIGESVKRFKKGDRVVIASGLGCGKCEQCINGRH
                     NLCPDVQVLGAKMDGAFAEYVHIEERHLTKLPENIPFDQGAILADAVSTPYHALKYVG
                     KIQTGDTVAIYGCGGLGIHAVTLARALGASKVIALDIDEGALENASKYKADELINLKK
                     VKNSGKALQEVSKGIDLMLDFSGYYSNIEDSIRAMNPGGRMVMAGIGKHPFKIGFPFM
                     VINKQISISGSLGCDARAIPELIELYSSGKLDLSTSITSHHPLEEVNECLEDLYHRKG
                     NPIRFIITPNGKI"
     gene            complement(1672..2088)
                     /locus_tag="PLF66_22965"
     CDS             complement(1672..2088)
                     /locus_tag="PLF66_22965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004501054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6285 domain-containing protein"
                     /protein_id="HMZ66967.1"
                     /translation="MQDRPDANALLEAIQDLLIKEVMPAIKDNDGLSYKTLVSWNMLG
                     VIGRELKYGEIYLDNEISRISNFLRENSISFVNEDIDDANYIAKMERCKSLNEKLTEY
                     IRKRKVANEDKDLWNLVKESLNEKLKIANPRFNAGE"
     gene            complement(2078..3118)
                     /locus_tag="PLF66_22970"
     CDS             complement(2078..3118)
                     /locus_tag="PLF66_22970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016558949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HMZ66968.1"
                     /translation="MSKEDLKSKLESYLTDRLRGNANVTNMIPLSGGACQDNYLLDLN
                     INNGEFSGEHRLVFRTDKGASLLASLSRIDEFKVCEITSSHGVKTPKPYWLETKEDVI
                     GSPFYFMERISGKATGRYVVKDPSLNKVRKKITEELAENLAKIHKITPNLCKDEDLRK
                     SLTRHSGKLPNSIALEAIEELRAQIPKLQEPHPAMELILNWLEKNAVSTDDEVLVHGD
                     FRTGNFMVSPNGLEGIVDWEFAHWGDRHEDVAWLCMRDWRFGKLNKEAGGFADRNEFY
                     SLYEKYSGIKLDPEKVTYWEIMGNIRWALGSAQQAERHLSGADKGIELAAIGRRTCEM
                     EFEAMRLIENAG"
     gene            complement(3121..4101)
                     /locus_tag="PLF66_22975"
     CDS             complement(3121..4101)
                     /locus_tag="PLF66_22975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66969.1"
                     /translation="MNQLKSLFNLLIFSGKKSTFFFLGVLLFLSYCATVPTQIVQHKF
                     HPVLTVKEFAPKEKTLLPAIVLYEPVLLKDTVLFRTEDKKEKGLIPFFLKNGYTVYLI
                     SSKETFPSFRNHALEFDSILNQIAEKQREREIYLGGVSLGGQAVLEYMTIPSSLENQA
                     RVSKIFFIGTGIDYNYSNSFTEHSTKKNYEETFVKDICPKGSKDNFCKRYIRFNNELQ
                     NMDNREKETFFDRIPKLKNDFLTRFHKTKLQLGYFLIYGKLDSISPEESIFPFFDKIR
                     SSSKMDSSNRMFEASEANGQSIDYDHADLFLYPDVEKEVYRRLLYWLKRI"
     gene            complement(4126..5199)
                     /locus_tag="PLF66_22980"
     CDS             complement(4126..5199)
                     /locus_tag="PLF66_22980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004497233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HMZ66970.1"
                     /translation="MTFKKIILFLILLILFPSLYQCSARIDLQGEIHHPKTSDNWDLT
                     LEHHSPRKLSSAKKFPVIICHGLIGNRNYFKIKEEESIVYRLISEGYDVWLLDLRGRT
                     DAGTPSLFFGEKTYNFSFDDYATKDVEAAISYVLAKTGAEKVNWIGHSMGGMVVYTKI
                     GTFQEKRIANLVTLGSPFSFYNPTKNVKTLNSLSFLLRFIPVIPSASIAHLGSDYKIP
                     LPEKPFLRLFYYKENTEPSVEHALKSVSTNNESPNVFKQFANGVKTGEILSLDESISY
                     TSNLKNIKIPVFLIAGRRDHLGTPMIVRHVYDNIGSNDKTMLIASRAEGHSEDYGHVD
                     LVMGTHSNKDINTPIVKWLNERN"
     gene            5275..6630
                     /locus_tag="PLF66_22985"
     CDS             5275..6630
                     /locus_tag="PLF66_22985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66971.1"
                     /translation="MKSNFIILFFLLSFFNLIWSDSIQAKKGYLINPNGIFLYKSKQF
                     KDKKGIIKQGEEFHISDELEDKDWIGVETKTQNGYIHNNDQDYIVFDRVLNNEYLVSD
                     EKQRILYEKPYLEANPISKIDGFSVLSVLEKESSKSPSKKKKEKQIWFKVKINNKIGF
                     LVSKNELYTSKESAMDVSQKKEIAIDGFALVIDPIYLKEPNGEIFTPKGASKKNEFVF
                     VVKERTDRKEKYFLVEMKNASFKNKMQATPIDFDEAPFQAWISETNAKYFTPTEFSYY
                     TLQKSSYKGDIRLLKNIFAANKDLPLNFQNVYFNPISDNKTDYFIVTLFKGYENSSGY
                     GEFYPVSFVVKKVKSDYKVYNGDLINSGKFQFSDLDRDGIPEVFTSMETVTYSRTAFS
                     APRFYAYIDEAYREIPLPGNFLDTYKITENFLYTQKEDNPKSKVQKYKYQNGEFIKIK
                     N"
     gene            6644..8194
                     /locus_tag="PLF66_22990"
     CDS             6644..8194
                     /locus_tag="PLF66_22990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66972.1"
                     /translation="MRKIENYSRVNFALILILFIFILENCKKEEAIEKSGNELNKTEA
                     KQANQDYAIILNQNGIPFYQTALDLKSKFDSIPFGGRIILTNENINSDSQEAGNWFSA
                     IYQGKKGFVFSDNAYSGKEWIQMSETKVNNEKTKQDEAAFSVVQSMNTVMYDLPSIES
                     KKIGEIPQFSLISILSSGYYFDSFFIDNIDLEMGQERLWYEVSYNGKNGYILNSINYP
                     VEKSVAEEQLRDKILSERGYFALTTKEPTLYNIDTKELFGKDFKVKLLKENIFFDTTE
                     SRLINGEQVYLIYFSDKMSLSPRKKPKNPAEDNTPYAAYISAKDGNYYSEEKFSEYTI
                     ENTKYKGDIAALNLFKSEFVKRGIYLNYLDFTVKKISGEGYPKDTYYIATAKVGNSGS
                     DFNASQARSVILKQSDGVYEAVSGSIYTSKPMQFKDLDGDGVMEIIAKTPNRGGDDTT
                     IFGLKDGKYVSVNSVLSQMGVNVIDIKGDKIRGSKWNTSTDEKQNKNEILLFKYDKGN
                     LVEIKNNK"
     gene            8339..9763
                     /gene="pyk"
                     /locus_tag="PLF66_22995"
     CDS             8339..9763
                     /gene="pyk"
                     /locus_tag="PLF66_22995"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017214512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HMZ66973.1"
                     /translation="MNFLNKKTKIVCTIGPASSSEKMISELIDAGMDVARMNFSHSDH
                     ASHKKVFDTIRLCEQRSGRPIGILADLQGPKIRTGKMKDGPFDVHPGDKIYINTKSDF
                     LGTREEISTTYKNLITDIEVGHKVLIDDGKILLNVIAKEMDRALLEVIVGGTIKDNKG
                     INLPGTPVTAPALSEKDVEDLKFALSLGVDYVALSFVRRASDLEIARQYMHGTFTGLI
                     AKIERPEAIKNIDEIIETADGIMIARGDMGVELDTERVPIIQKELIRKMNLVGKPVIT
                     ATQMLESMIDNPRPTRAEASDVANAVLDGTDAVMLSAESASGKYPLESVTIMSKIIHE
                     AETIFMNLGVQRRANRPTDVTERVALGTAAEQISDSIGAKAIVNFTRSGLSARIASQF
                     RPKVPIFSFTPFLMTARKMNLLRGVYPSIIPIMDKFPDMINYMHQKLSSDGLVSKGDR
                     AVILSGTPGGEANTVDFVQIYHFK"
     gene            9771..10259
                     /locus_tag="PLF66_23000"
     CDS             9771..10259
                     /locus_tag="PLF66_23000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66974.1"
                     /translation="MKFFLIGLVLLLAGFSLTAQEKNTQKPNKLESEKEKGKEKQEKK
                     ETDNTKSEFDSKSANSVPFIGANVGSVWTVDEVKNTLIYSPDSMMKKSKYKRFIFDLK
                     EFRKLGGNTKPEADFTIYYNVLQTDIENSKPENPNMQSPIDGFTFIINKCKIEKVEVN
                     TP"
     gene            complement(10256..>10570)
                     /locus_tag="PLF66_23005"
     CDS             complement(10256..>10570)
                     /locus_tag="PLF66_23005"
                     /inference="COORDINATES: protein motif:HMM:NF020133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HMZ66975.1"
                     /translation="WMQFFQKKDFMLLERAVEDKVHTPYRINSIPLLVNVAELCKKLG
                     AAYSLSGSGPSVLIYIEKKHAKKFLAKFQDELSKKNSNNIAYTIHPIEPDHAGTIIKE
                     KG"
BASE COUNT         3566 a         1741 c         1807 g         3456 t
ORIGIN      
        1 ttgcactctt ccaaattgcc caggaggaaa cggagaacat ataggctgga tttttcaagc
       61 gaataaaaat tattaccaag cagatggaat tactttgatt gggacgacag acgcaaatgg
      121 tctaatgcca cccacatttg taaacccagc aactggaaac gcaaagtatt ggacgggaat
      181 aaatgtagat tggacaacag cagttgcgaa taattgtgtc aattggacaa gtaatgcggg
      241 tggaatttat ggaaattgtg gtggatctgg ttctggtccg aatacatctc ctggtaattt
      301 attaaatagt tggggtggca ctgttgcctg taatgcacca caacaacttt tatgtataga
      361 acagtaaatc accaaatttt aatgatttgc cttggtttat tttagtttaa ttaaaatcga
      421 attagatttt tccatttggt gtaataataa accgaattgg atttcctttt ctgtgataca
      481 aatcctctag gcattcattt acttcttcga gtggatgatg agaagttatc gatgtggata
      541 aatctaattt gccgctggaa tagagttcaa ttaactcagg aatggctctt gcatcgcaac
      601 ctaaggaacc agagattgaa atttgcttat tgatgaccat aaaaggaaat ccaattttaa
      661 acggatgttt tccaattcca gccataacca ttctgccccc agggttcatt gctcgaatag
      721 agtcttcaat attagaataa taaccagaga aatctaacat tagatcgata cctttcgata
      781 cctcttgcaa agcctttccg gaatttttaa ctttctttaa attgattaat tcatcagctt
      841 tgtatttgga agcattctca agagctccct catcaatatc gagagcaatt actttgctag
      901 cacctaacgc acgtgccagt gtcaccgcat gtattccaag tcctccacag ccataaatag
      961 caacagtgtc acctgtttga atttttccaa cgtatttaag tgcgtgatag ggagtagaga
     1021 cagcatcagc taggattgct ccttgatcga atggtatatt ttctggaagt ttggttaaat
     1081 gtctctcttc aatatgaaca tattctgcaa atgctccatc catttttgct ccaaggactt
     1141 gaacatcggg gcataagtta tgtctgccat ttatacattg ttcacatttc ccacaaccaa
     1201 gaccagaagc aattacaacc cgatcccctt ttttaaatct ttttacactc tctccgattt
     1261 cgcttacaac tccggaggct tcatgtccta atattgtagg gtagtgtttg agtcttactt
     1321 gtttgtgaat agtcatgtga atatctgatc cacatacccc acagcattta atatctacct
     1381 ttacttggtt tggttgtaac tcaggcaagg taacatcttc aatttttaaa tgagatgcgt
     1441 ttgaataaag aactcctgct ttcattgaat actccttgta tgaaaaagat tttcacctat
     1501 tgccacaact agcggcaatg gattttgact ggaaacacag ataaaagaag ggtaaacacc
     1561 gatgcgctta tgattcaatt tcgagtaagc ctcatttaat ccatgccttt tgatcctaca
     1621 aattttaatt aaactacttt cggttttcat cggtggtaaa tctaaaatgt tttattcccc
     1681 tgcgttaaag cgagggttgg cgatctttag tttttcgttt aacgattctt ttactagatt
     1741 ccaaaggtct ttatcttcat ttgctacttt tcttttgcgg atatattctg ttagcttctc
     1801 atttaaagac ttacatcttt ccattttcgc gatatagttt gcatcatcta tgtcttcatt
     1861 tacgaatgag attgaatttt ctcttaaaaa attagaaatt ctactaattt cattgtctag
     1921 atagatttct ccatatttta attcgcgacc aattacaccg agcatattcc aactcactaa
     1981 ggtcttatag gaaagcccat cattgtcttt gattgctggc attacttctt ttataagtaa
     2041 atcttgaatt gcctctaaaa gtgcatttgc atccggtcta tcctgcattt tctattaacc
     2101 tcattgcttc aaattccatt tcacaagttc ttctaccaat agccgcaagt tcgatccctt
     2161 tatcagctcc acttaaatgt ctttctgcct gttgtgcaga gccgagtgcc cagcggatat
     2221 ttcccattat ttcccaatag gttacttttt ctggatctag tttaatacct gaatactttt
     2281 catagagaga ataaaattca tttcgatctg caaagccacc ggcttcttta tttaattttc
     2341 caaatctcca atctctcata cataaccaag ctacatcttc gtgtctatct ccccaatgtg
     2401 caaattccca atctacaatg ccttctaagc cgttaggtga taccatgaag ttacctgttc
     2461 taaaatcacc atgaactaaa acctcatcat ctgtagatac tgcatttttc tcaagccagt
     2521 ttaagattaa ttccattgct ggatgtggct cttgtagttt tggaatctga gccctcagtt
     2581 cttcgattgc ttcaagcgca atactgttag gtaattttcc agaatgccta gttaagcttt
     2641 ttcttaaatc ctcatcttta caaagattgg gagtaatttt atgaattttt gctaggtttt
     2701 ctgccagttc ttctgtaatt ttttttcgaa ctttatttag agaaggatct tttactacat
     2761 aacgacctgt tgctttacct gaaatacgtt ccatgaaata gaaaggactt ccaatcacat
     2821 cttcttttgt ttcaagccaa taaggcttcg gagtttttac tccatgacta gaagtaattt
     2881 cacaaacttt aaattcatca atcctagaaa gacttgctaa aagagaagcg cctttatcag
     2941 ttcgaaatac caatctatgc tcaccgctaa actctccatt gtttatgttt aagtcgagta
     3001 ggtaattatc ttggcatgca ccaccactca gaggtatcat gtttgtaacg tttgcattgc
     3061 ctctgagtct atcggttaaa taagattcta atttgctttt taagtcctct tttgatatag
     3121 tcatatgcgt tttaaccaat agagtagtct tctatagact tctttttcaa catcaggata
     3181 tagaaacaaa tctgcatgat catagtcaat agactgacca tttgcttcac ttgcttcaaa
     3241 catacggttc gaagaatcca ttttagagct agagcgaatt ttatcaaaga aaggaaaaat
     3301 agattcttcg ggactgatgc tatctaattt cccataaatc agaaaatatc caagttgtaa
     3361 ttttgtttta tgaaatctag taagaaaatc atttttcaac tttgggattc tatcaaaaaa
     3421 agtttccttt tctcgattgt ccatattttg taattcgttg ttaaaacgga tataacgttt
     3481 gcaaaaatta tctttgcttc ctttcggaca aatatctttt acaaaggtct cttcataatt
     3541 cttttttgtc gaatgttcgg taaaactatt tgaatagtta taatctattc ctgttccaat
     3601 aaaaaagatt tttgaaacac gcgcttggtt ttctaagcta ctaggaattg tcatatattc
     3661 taaaactgcc tgtccaccga gagaaactcc acctaaataa atttctcttt ctctttgctt
     3721 ctctgcaatc tggtttagaa tcgaatcaaa ctccaaagca tgatttctaa aacttggaaa
     3781 tgtttctttg gaggaaatta gatacacagt atagccgttt tttagaaaga aaggtattaa
     3841 acctttttct tttttatctt ctgttctaaa aagcacagtg tcttttaaaa gaactggttc
     3901 atataaaacg attgcaggca ataacgtttt ttctttcggt gcgaactctt ttactgttaa
     3961 aacaggatga aatttgtgtt gcacaatttg tgttggaaca gttgcacaat aacttagaaa
     4021 gagtaaaact cctaaaaaga aaaatgttga tttctttccc gagaatatta atagattgaa
     4081 taatgatttt aactgattca taaaattcct actttataag gtaaattaat ttctttcatt
     4141 gagccatttg acaataggag tgtttatatc tttatttgag tgagttccca ttaccaaatc
     4201 tacgtgacca taatcttcac tatgaccctc tgcacgacta gcgattaaca ttgttttgtc
     4261 gttactacca atattgtcat acacatgtcg cacgatcatc ggtgttccaa gatgatctct
     4321 tctgcctgct attaaaaata ctggaatttt tatattcttc aaattagatg tataagaaat
     4381 actttcatct aaggaaagaa tctcgcctgt tttcactcca tttgcaaatt gcttaaaaac
     4441 atttggactt tcattgtttg tgctgacgct ctttaaagca tgttctacgg atggttctgt
     4501 gttttctttg tagtaaaaca atcttagaaa aggtttttct ggaagtggga ttttatagtc
     4561 tgatcctaga tgtgcgatac ttgcagaggg gataacgggt ataaaccgca gcagaaaaga
     4621 aaggctattt aaggtcttta catttttggt tggattgtaa aaggagaagg gcgaacctaa
     4681 ggtgactaaa tttgcgattc ttttttcttg aaaggttcct atttttgtgt aaacaaccat
     4741 tcctcccata ctatgaccaa tccaatttac tttctctgca cctgtttttg ctaatacata
     4801 actaattgct gcttccacat ctttggtcgc ataatcatca aaactaaagt tgtaagtttt
     4861 ttctccaaaa aataaagaag gtgttcccgc atctgttcta cctcttaaat caagtaacca
     4921 aacatcatac ccttctgaga tcagtctata tacaatggat tcttcttctt taattttaaa
     4981 atagtttcta tttccaatta aaccatgaca gataatgact ggaaattttt tcgcagagct
     5041 aagcttgcgt gggctatgat gctctagtgt taaatcccaa ttgtcagagg ttttgggatg
     5101 gtggatttct ccttggagat caatccgagc tgagcattga tagagagaag ggaacaaaat
     5161 caataagatt aaaaagagga taattttttt aaatgtcatt ttttttcctt taccgagaga
     5221 ttggaatgat ataatttcct gtaaagatag agtaaatttt ttaaatagcc ttttatgaaa
     5281 tcaaatttta taattttatt ttttttatta tcttttttta acctaatttg gtccgattct
     5341 attcaggcta aaaaaggcta tctcataaat ccaaatggaa tttttcttta taaatcaaaa
     5401 cagtttaaag acaaaaaagg tatcataaaa cagggtgagg aatttcatat atcggatgaa
     5461 ttagaagata aagactggat tggagtcgaa accaaaactc aaaatggcta tattcacaat
     5521 aacgatcagg attatatagt cttcgatcga gttctgaata atgaatactt agtctcagat
     5581 gaaaagcaga ggattcttta tgaaaagcca tatcttgagg ctaatcctat atccaaaata
     5641 gatggattta gtgttctttc tgttttagaa aaagaatctt ctaagtctcc atctaagaaa
     5701 aaaaaagaaa aacagatttg gtttaaagtc aaaataaaca acaaaatagg ttttctagtt
     5761 tctaaaaacg agctttatac ttccaaagaa agtgcaatgg atgtttctca gaaaaaggaa
     5821 attgcaatcg atggttttgc ccttgttata gatccaattt atttaaagga gccaaatgga
     5881 gaaattttta ctcccaaagg agcttcaaaa aaaaatgaat ttgtctttgt tgtgaaagaa
     5941 agaacagatc gtaaggaaaa atatttttta gtcgaaatga aaaatgcatc cttcaaaaat
     6001 aaaatgcagg caaccccaat tgattttgat gaagctccat ttcaagcatg gatttcagaa
     6061 accaatgcta aatattttac tccaacagaa tttagttact atacgttaca gaaatcttcc
     6121 tataaaggag atattcgatt attaaaaaat atttttgcgg caaacaaaga tttaccgtta
     6181 aattttcaaa atgtatactt taatccaatc tctgataaca agacagatta ttttatagta
     6241 acgctattta aaggttatga aaattcttcc ggttacggag aattttatcc tgtttcattt
     6301 gttgtaaaaa aagtaaaatc agattataaa gtctataatg gagatcttat taattctgga
     6361 aaatttcaat tcagtgattt ggacagagat ggtattcctg aagtatttac ctcaatggaa
     6421 acagtaacct attctagaac agctttttca gctcctcgct tttatgcata cattgatgag
     6481 gcttacagag aaatacctct accaggaaat tttcttgaca catataaaat aactgaaaac
     6541 ttcctttata cccaaaaaga agacaatcca aaaagtaaag ttcaaaaata caaatatcag
     6601 aacggcgaat ttataaaaat aaaaaactag tgtgaggtgt tttatgagaa aaattgaaaa
     6661 ttatagcagg gtaaattttg cattaatatt aattcttttt atatttattt tagaaaactg
     6721 caagaaagaa gaagcaatag aaaagtcggg aaacgaatta aataaaacag aagcaaaaca
     6781 ggctaatcag gattatgcga ttatcttaaa tcaaaatgga atcccatttt atcagactgc
     6841 actcgatcta aaaagtaaat ttgatagtat cccttttggg ggaagaatca tattaaccaa
     6901 tgaaaatatc aactctgatt ctcaagaggc agggaattgg tttagtgcaa tttatcaggg
     6961 aaaaaaagga tttgtttttt cagataatgc ctattcggga aaagagtgga ttcaaatgag
     7021 cgaaacaaag gtaaacaacg aaaagacaaa acaagatgag gcggcttttt ctgttgttca
     7081 atctatgaat acagtaatgt atgacttacc ttctatagaa tcaaaaaaaa taggagagat
     7141 tcctcagttt agtttaatct ctattctctc gagtggatat tattttgatt ccttttttat
     7201 agataatata gatttagaaa tgggtcaaga gagattatgg tatgaagttt cgtataacgg
     7261 taaaaatggt tatattttaa atagtattaa ttatccagtt gaaaaatctg tagcagagga
     7321 gcaattaaga gataaaattc tatcggagag aggttatttt gctctcacaa caaaagaacc
     7381 aactttatac aatatagata caaaagaact ttttggaaaa gactttaaag ttaaactcct
     7441 aaaagagaat attttttttg acacaaccga atctagactc ataaatggag aacaagttta
     7501 tttgatttat ttttctgata aaatgagtct atctcctaga aaaaagccaa agaatccagc
     7561 agaggataac actccatatg ccgcatatat ttctgcaaaa gatggaaact attattcgga
     7621 ggaaaaattt tctgagtata cgattgaaaa tacgaaatac aaaggagaca ttgctgcttt
     7681 gaatcttttt aaatcagaat ttgtaaaacg gggaatttat ttaaattatc tggattttac
     7741 agtaaaaaaa atctcaggag aaggttatcc aaaagatact tattatattg caactgcaaa
     7801 ggtaggaaat agcggtagcg actttaatgc aagccaagct agaagtgtta ttttgaaaca
     7861 atctgatgga gtctatgaag ctgtatcggg aagtatttat actagtaagc caatgcaatt
     7921 taaagactta gatggtgatg gtgttatgga aatcatagca aagactccaa atcggggagg
     7981 cgatgatact acaatctttg gattaaaaga tggtaaatat gtttctgtta acagtgtttt
     8041 atctcaaatg ggtgtaaatg taatcgatat aaaaggagat aaaattcgtg ggtcaaaatg
     8101 gaatacatct acggatgaaa agcaaaataa aaatgaaatc ctattattca aatatgataa
     8161 agggaattta gtagaaataa aaaacaataa atagagcgat agttgaatcg aagaaatagg
     8221 aaatggaaga ttttacgagt gttaataaaa aaacattcat tagtagaaaa ataaaatttc
     8281 ttcttcgaaa aatgaacgtg acaaaaagga taaggtaact taaatcaagc ttatgaacat
     8341 gaactttcta aataaaaaaa ccaaaatagt ttgcactata ggtccagctt cttccagcga
     8401 aaaaatgatt tctgaattaa tagatgcagg aatggatgtt gcgagaatga atttttctca
     8461 ttcggatcac gcaagtcaca aaaaagtttt tgatacaatt cggctctgtg aacaacgttc
     8521 tgggagacca atcggaattc ttgccgatct acaaggacca aaaattcgca caggtaaaat
     8581 gaaagatgga ccatttgatg ttcatccagg tgataaaatt tatattaata caaaatccga
     8641 ttttttggga actagagaag aaattagcac tacttataaa aatttaatca ctgatattga
     8701 agtaggtcat aaagttttaa ttgatgatgg aaaaatttta ttaaatgtaa ttgccaaaga
     8761 aatggataga gctctcctag aagtgattgt aggtgggact atcaaagata ataaaggaat
     8821 taaccttcct ggaacacctg taactgctcc agcgttatcc gaaaaagatg tagaagattt
     8881 aaaatttgcg ctttctttag gagtagatta tgtagcgctt agcttcgtta gaagagcctc
     8941 tgatttagaa attgcaagac agtatatgca tgggactttt acaggactca ttgctaagat
     9001 cgaaagacct gaagcaatta aaaacattga tgaaatcatt gaaacagctg acggaatcat
     9061 gatagcaaga ggtgatatgg gagtcgaatt agatacagaa agagttccaa tcattcaaaa
     9121 ggaactaatt cgtaaaatga atttagtagg caaaccagta atcactgcaa cccaaatgtt
     9181 agaatctatg attgataatc cgagaccaac gagagcagaa gcgagcgacg tggctaacgc
     9241 ggtgttagat ggaacggatg ctgtgatgtt atctgcagaa tcagccagtg gtaaatatcc
     9301 attagagtca gttactatca tgagtaaaat cattcatgaa gcagagacaa ttttcatgaa
     9361 cttaggtgtg caaagaagag caaataggcc tactgatgta acagaaagag tcgcactcgg
     9421 aactgccgca gaacagattt ctgattcgat aggcgccaaa gcaattgtga actttactag
     9481 aagtggtctt tccgcaagaa ttgcttctca gtttagaccc aaggttccaa tattttcttt
     9541 tactcccttt ttaatgactg caaggaagat gaatctatta agaggagtct atccttcgat
     9601 tattccaatt atggataaat ttcctgatat gatcaattat atgcatcaaa agttatcttc
     9661 tgatggattg gtttcgaaag gagatagggc ggttattctt tccggaacac caggtggaga
     9721 ggcaaacacg gtagattttg tacaaatcta tcattttaaa taggaatgtt atgaaatttt
     9781 ttttaatagg tttagtttta cttcttgcag gattttcatt aactgctcaa gaaaaaaaca
     9841 cacagaaacc aaacaagtta gaatcggaaa aagaaaaagg aaaggaaaaa caagaaaaga
     9901 aagaaacaga taatactaaa tctgaattcg attctaaatc agcaaatagt gttcccttta
     9961 ttggagccaa tgttggctcc gtttggactg tagatgaggt taaaaatact ctcatctata
    10021 gccccgattc aatgatgaaa aaaagtaaat ataaacgttt tatatttgat ttaaaggaat
    10081 tcagaaaact agggggcaat actaaaccag aagcagattt tactatctat tacaacgtat
    10141 tacaaacaga tattgagaat agcaaacctg aaaatccaaa tatgcaatca cctatcgatg
    10201 gttttacttt tattatcaat aagtgtaaaa ttgaaaaagt agaggttaat actccttaac
    10261 ctttctcttt tataattgtg cctgcatgat ctggttcaat cggatggatt gtgtaggcta
    10321 tattatttga gtttttcttt gaaagttcat cttgaaactt tgctaaaaat ttcttagcat
    10381 gttttttttc tatataaatt aatacgctcg gaccactgcc agacaatgaa taagcagcac
    10441 ctaatttttt gcatagttct gcaacattta ctagtagggg aatagaattt atacgatacg
    10501 gtgtatgaac cttatcctca acagctcttt ccagtaacat aaaatccttt ttttgaaaga
    10561 attgcatcca
//
LOCUS       DAOQOS010000161        10420 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_3348_length_10420_cov_6.275929, whole genome shotgun sequence.
ACCESSION   DAOQOS010000161 DAOQOS010000000
VERSION     DAOQOS010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 10420)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10420)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10420
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3348_length_10420_cov_6.275929"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..810
                     /locus_tag="PLF66_23010"
     CDS             <1..810
                     /locus_tag="PLF66_23010"
                     /inference="COORDINATES: protein motif:HMM:NF013660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase"
                     /protein_id="HMZ66976.1"
                     /translation="KPIKIGHTGTLDKAAEGLLILPFGDYTAFSSYFLEADKGYYAQV
                     GFGKSTDSGDRDGVIQNEWDFARTKEFFLSNQESIRQEIEKIIDTNLQTPPVISAIKV
                     GGIRQSSLYREGISFESVARKIKIYKFNYENLDETGFTFHTLVSSGTYIRKIVIDLSE
                     KLDFPMYLKKLVRTSLGELTLEKALSSEEILSPDAKHFTLEEMIPYPWIDLNEKETHS
                     VRHGGYVRQNVNGNFLLRDFQGRLLAWCSTEDKKPHLPYKYLKVFYNPDEN"
     gene            868..1134
                     /gene="rpsO"
                     /locus_tag="PLF66_23015"
     CDS             868..1134
                     /gene="rpsO"
                     /locus_tag="PLF66_23015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010575366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HMZ66977.1"
                     /translation="MITTETKKSIITEYQTKAGDTGSPEVQIALLNSRINDLNLHFAA
                     HKKDHHSRRGLFKLVSQRKKMLDYLKSKNLERYRTLIKRLGLRK"
     gene            1150..3255
                     /gene="pnp"
                     /locus_tag="PLF66_23020"
     CDS             1150..3255
                     /gene="pnp"
                     /locus_tag="PLF66_23020"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004497609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HMZ66978.1"
                     /translation="MIHKHTLNFDREAISIETGNWAKQADGSVVYRIGNLVMLATVCA
                     LDDSKEGQDFFPLTCEYTEKMYSVGKIPGGYVKRESKPSEYEILISRIIDRPIRPMFP
                     EGYFCEVQLIVTVLSADKNISTAGHAVNAASAALMVSDIPFHGPLAGVRVGRIDGKLV
                     YNPENDSMSKSDMDILVAGTSDSIVMIEGECKELSNQELLEALKFAHEYIKANVSFQS
                     EFTKIVNKPKREIKVKVKDEALFNRVRDFALEKISVANRNSDKQARSKGISDANEETI
                     KYFTEQNLTELELKDVKNFLHEIEAEVVRDLIFKEGLRADGRKLEEVRDISCELNVLP
                     GAHGSAVFTRGQTQSLGVVTLGTVADNQRYETIEGQKEKNFMLHYNFPAYSVGEVKRS
                     GSPGRREIGHGNLAERALKAVLPKFTDFPYVIRLVSEILESNGSSSMASVCSGSLAMM
                     SGGVPIKAAVSGVAMGLITSPNKEFAILTDIAGIEDHFGDMDFKLAGTRKGITAFQMD
                     LKITGVGLDVLERAIAQAERGRNHILNEMEKSISQSASELSKTAPRITIRMIPKDRIG
                     ELIGPGGKNIRSIIEQSKADINIDDDGKVTISSPDAESAKKAVDIIEGMFAEVEVGKI
                     YEGKIKRITDFGAFVEILPGKEGLCHISKLDFKRVNAVKDVVSEGEIIRVKVLGVDRQ
                     GKIDLSRKDAMQQEQHSAK"
     gene            3252..4520
                     /locus_tag="PLF66_23025"
     CDS             3252..4520
                     /locus_tag="PLF66_23025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HMZ66979.1"
                     /translation="MNLRLIKSNLSNGLTVLFQPVPNATSVSIGLWVKIGSRYESDDE
                     KGYTHFLEHMLFKGTEKRSSKKQAEDIERVGGFFNAVTSREYTYYYVTVASPELELGL
                     DILSDMVFSPLLREQDIRNESSVILEELKSYEDSPDDYVYDEYFKNIFNNHPLGRDII
                     GTRESVAGVTENSIQSYYKKHYVPGRMVLSIAGDFPEDEIKRLTEKYFSIKKESPLVP
                     LENSPVTKSFSIHFIKRKLEQVNFLLGAEGFPRDFTTAVRLGVIANILGGGMASRLFQ
                     KIREEKGLCYSISCFPSSYLDTGIMNINCATSKDKFLNAVESILEEIRLLKKDGFSQE
                     ELDFAKSNQRGGLAIGYEIPENRMTDIAMQELYFGRFYNYENRLEELASVTLDEINDL
                     VGKVFSVDQMHFTGIGNIPKVDIKKIHTKI"
     gene            4549..5001
                     /gene="dut"
                     /locus_tag="PLF66_23030"
     CDS             4549..5001
                     /gene="dut"
                     /locus_tag="PLF66_23030"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HMZ66980.1"
                     /translation="MDAVNIKIQKFFPDVKLPEKKTSGAAALDVSAYCPKANIQIDVG
                     QTVLIPTGFCLEIPEGYFVEIRPRSGFSTKENIIIPNSPGTIDSDYRGQVFIPLLNLS
                     QKPFQITNGLRIAQMLVAKSISIEWNLVDEISLDTERGTGGFGSTGRH"
     gene            complement(5206..5823)
                     /locus_tag="PLF66_23035"
     CDS             complement(5206..5823)
                     /locus_tag="PLF66_23035"
                     /inference="COORDINATES: protein motif:HMM:NF022744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3102 domain-containing protein"
                     /protein_id="HMZ66981.1"
                     /translation="MSKKKTSNSVELVTSGKLSKDRDETIKEIEVLHFKFLENARNHL
                     LLAKQIGERLEEKKTTVKHGEWEAWVEANLPFKIRTAQYYMKIHKNWNTLITSSAKSI
                     GLLTEAIELITEKKSLQNLEIAEKLKLEKREKRQDILSLHRRFFKKGENLNQRDRKAI
                     YHFLEDIRVKREEKLKGYQTKIEKTKQALTENKKQMRKVLESLKE"
     gene            complement(6493..7149)
                     /locus_tag="PLF66_23040"
     CDS             complement(6493..7149)
                     /locus_tag="PLF66_23040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66982.1"
                     /translation="MKKIVALIFYITFPFWALSKEQMTYTFKAHNFRIDLPTHWELRK
                     AKTSSLKKPLFTAYSKEDRIHLEVYTTNYQKRDMQEGFILTLKNLQLWAENYTSLDQE
                     AEFPLYHLKGYWAKTMGYKQKRKIFGYLVYATDGVNLFIIHSYTQANLFPKNFLSMKE
                     IFKGFSLNRNYKNECCNECITLSLEGIENQKASCANFTETNECMFFFQNQPMSVLECN
                     "
     gene            7304..8311
                     /gene="gap"
                     /locus_tag="PLF66_23045"
     CDS             7304..8311
                     /gene="gap"
                     /locus_tag="PLF66_23045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008592325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HMZ66983.1"
                     /translation="MTKIAINGFGRIGRLVLRVGLEDPNLEFVAINDLVPPDNLAYLF
                     KYDSTHGKYKGTVEHDAENLIINGKKIKCLAEKEPEKLPWKNLGVDYVIESTGRFTDK
                     EGAGKHLTAGAKKVIISAPAKDKDIPTYVLGVNESKYNPATDHIVSNASCTTNCLAPL
                     VKVVLDNFGFAEGLMTTVHAMTATQPTVDGPSKKDFRGGRGAAQNIIPSSTGAAKAVG
                     LCIPEVAGKLTGMSFRVPTPDVSVVDLTCKTVKPTSLKEIKQKMKEAAEGNLKGILGY
                     TEEAVVSNDFTSSTLSSVFDADASIELNANFFKLVSWYDNEMGYSNRVVDLIRYMVKK
                     G"
     gene            8313..9500
                     /locus_tag="PLF66_23050"
     CDS             8313..9500
                     /locus_tag="PLF66_23050"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020774321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HMZ66984.1"
                     /translation="MNLPRIENVDVKGKRVFLRVDFNVPLENGKVSDKTRIEKTLPTI
                     ELLLNRGAKLIVASHLGRPDGKVDSRYSMKPVQEAFEAILGKPVKFSEQIVGSEVEKL
                     SQDLKDGEVLLLENLRFHKEEEENDPAFSKKLAQLAEIYINDAFGAAHRAHASTEGIA
                     HHLPSYAGTLMYREIEVLTSLITKPEKPFVAIIGGAKVSSKIKILKNLFNKVDTMLIG
                     GGMAYTFLKSRAVPIGSSLAEKEFEPQAFQIIDKAAYENVDFQLPIDHVIADSFSEKA
                     KTKTVDKMGIIDGWMGMDIGPKTISAYEKIIKSAGTILWNGPMGVFEMEKFSNGTIQI
                     AKAIAKSNAKSVVGGGDSIAAINKAGVEDKITHISTGGGASLEFLEGKTLPGVAALLK
                     NKD"
     gene            9503..10249
                     /gene="tpiA"
                     /locus_tag="PLF66_23055"
     CDS             9503..10249
                     /gene="tpiA"
                     /locus_tag="PLF66_23055"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020770742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HMZ66985.1"
                     /translation="MRKKIMSGNWKMNLNLAQATELATGIKKGLESKKTSLDLLVFPS
                     SIHLEKVSEILKGTSVKVGAQNIYPSGLAAFTGEISTDQLKDLGISHALVGHSERRQF
                     LGETDSFLNQKILFLLKAGFTPVFCIGETLQEREANKTFDVVKRQVVEGLKSVSASDM
                     KNIIIAYEPVWAIGTGKVATPEQAEEVHAFIRKELAALYDTTVSDSVSILYGGSVKPD
                     NVKALLEKPNIDGGLVGGASQKWDSYLALL"
     gene            10291..>10420
                     /gene="secG"
                     /locus_tag="PLF66_23060"
     CDS             10291..>10420
                     /gene="secG"
                     /locus_tag="PLF66_23060"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HMZ66986.1"
                     /translation="MGFLIGTVLVFFVISCVFLILLVLVQAGKGGGLGGMVGGASQT"
BASE COUNT         3445 a         1650 c         2036 g         3289 t
ORIGIN      
        1 aaaccaatta aaattggtca tacaggaact ttagacaagg cagcagaagg tcttttgatt
       61 ttgccatttg gagattatac tgcgttttca agttatttcc tagaagcgga taagggatat
      121 tatgcgcagg taggctttgg aaaaagcacg gactcgggag atagagatgg agtaattcag
      181 aatgaatggg acttcgctag aacaaaagaa tttttccttt caaatcaaga gtcgattcgg
      241 caggaaatag aaaaaatcat tgatacgaat ctccaaactc ctcctgtgat ttctgccata
      301 aaagttggtg gtattcgtca atcctctctc taccgagaag gtatctcttt tgaatccgtt
      361 gcgcgtaaaa ttaaaattta taaatttaac tatgagaatt tagatgaaac aggctttacc
      421 tttcatacac ttgttagttc aggaacgtat atccgaaaga tcgtgattga tctttcagaa
      481 aaattagatt ttccaatgta tttaaaaaaa ttagtcagaa cctctcttgg agaattgact
      541 ttggaaaaag ctttatcgtc tgaggagatt ctctctcctg atgcaaaaca ttttactcta
      601 gaagagatga ttccttatcc atggattgat ctcaatgaaa aagaaactca ctctgttagg
      661 catggtgggt atgtaagaca aaatgttaat ggaaattttt tgctcagaga ctttcaaggc
      721 agattacttg cttggtgtag cacagaagat aaaaaacctc atttacccta taaatatctt
      781 aaagtttttt acaatccaga tgaaaattag tttatcaaac accctcgtta aataatttgg
      841 tctaaaatag aagaaaacta acacaatatg attacaacag aaactaaaaa atctatcatc
      901 actgaatacc agactaaagc aggggacaca ggttctccag aagtgcaaat cgcgctatta
      961 aatagcagaa tcaatgattt aaacctacat tttgcagcac ataaaaaaga ccaccattct
     1021 agaagaggtc tttttaaatt agtcagccag agaaaaaaga tgctggacta cttaaaatct
     1081 aaaaatcttg agagatatag aacattgatc aagagacttg gattgagaaa gtaattcagg
     1141 atctaaaaca tgatacataa acacacctta aattttgata gggaagctat atccattgag
     1201 acaggcaatt gggcgaagca agctgatggt agcgtagttt accgtattgg aaatctagta
     1261 atgctcgcaa ctgtttgcgc tctagatgat tccaaagaag gacaggattt ctttccactt
     1321 acttgcgaat acactgaaaa gatgtattct gtaggaaaaa ttcctggcgg atacgttaaa
     1381 cgggaaagca aaccttctga atatgaaatt ctaatttcta gaattatcga tagaccgata
     1441 cgtcctatgt ttccggaagg atatttctgc gaagttcagt taattgtaac tgttctctct
     1501 gctgacaaaa atatatccac agcgggacat gctgtaaatg cagcaagtgc agctcttatg
     1561 gtttccgata ttccatttca tggacctcta gctggagtta gggtgggaag gattgacggt
     1621 aaattagtct ataatcctga aaatgatagc atgagcaaat ccgatatgga tatactcgtt
     1681 gcagggacaa gtgactccat tgtaatgatt gagggagagt gtaaagagct gtccaaccaa
     1741 gaattattag aagcgctaaa atttgcacat gaatatataa aagcaaatgt atcctttcag
     1801 tctgaattca caaaaattgt aaacaagcct aaaagagaaa tcaaagttaa agtaaaggat
     1861 gaagctttat ttaacagagt aagagacttt gctctcgaaa agatttctgt tgcaaaccga
     1921 aattctgata aacaagcacg ttcaaaggga atctccgatg caaatgaaga aacaataaaa
     1981 tattttactg agcaaaatct gactgaacta gaactcaaag atgtaaaaaa ctttttacat
     2041 gaaatagaag cagaagtagt cagagattta atcttcaaag aaggcttaag agcggatggt
     2101 agaaaattag aagaagtaag agatatttcc tgtgaattaa atgttcttcc gggtgctcat
     2161 ggttcggcgg tatttactcg cggtcaaact cagtctttag gtgttgtaac acttggaacc
     2221 gttgcagaca accaacgtta tgagaccatt gaaggtcaaa aagaaaagaa ctttatgctt
     2281 cattataact ttcctgctta ttcagtggga gaggtgaaga gatctggttc cccaggtaga
     2341 agagaaattg gacatggaaa tttagcagaa cgtgctttaa aggcagtcct tcctaaattt
     2401 actgatttcc cgtatgtaat acgtcttgtt tctgagatct tagagtctaa cggttccagt
     2461 tctatggctt ctgtttgcag tggatctctt gcaatgatgt ctggtggagt tccaattaaa
     2521 gctgccgttt ctggagtagc tatgggattg attacttctc caaacaaaga gtttgctatt
     2581 ttaaccgata tagctgggat agaagatcat tttggagata tggatttcaa attggctgga
     2641 actagaaaag gaatcaccgc tttccaaatg gatctaaaaa ttactggtgt aggtctagat
     2701 gttctagaaa gagcaatcgc acaagcagag agaggaagaa atcatatttt aaatgaaatg
     2761 gaaaaatcca tttctcaatc tgcatccgaa ctttctaaaa cagctccacg tataacgatt
     2821 cgaatgattc caaaagatag aattggagaa ttaattggtc ctggcggaaa aaatatccga
     2881 tctatcatcg aacaatccaa agcggatatc aatatagatg atgatggtaa agtaacaatt
     2941 tctagtccag atgctgagtc tgcgaagaaa gctgttgata tcatagaagg tatgtttgca
     3001 gaagtagaag ttggtaagat atacgaaggt aaaatcaaac gtattactga ctttggagct
     3061 tttgtagaaa tacttcctgg aaaagaagga ctttgccata tttctaagtt agattttaag
     3121 cgtgtcaatg ctgtgaaaga tgtagtatcc gaaggggaaa tcattcgagt caaagttctt
     3181 ggcgtagaca gacaaggaaa aattgactta tcccgtaaag atgctatgca gcaggagcag
     3241 cactctgcta aatgaattta aggctaatca agagtaacct ctctaatggt ctaacagttt
     3301 tatttcaacc agttcccaat gcgactagtg tttcgattgg gctctgggta aaaattggtt
     3361 ctaggtatga gtctgacgac gaaaaaggat acactcactt cctagaacat atgcttttta
     3421 aaggaactga aaagcgttct tccaaaaaac aagcagaaga tattgaaagg gtaggcggtt
     3481 tttttaacgc tgttacttct cgtgagtata cgtattatta tgtgactgtc gcttctccgg
     3541 aattggaact aggactcgat atcctttctg atatggtttt ttcacctctt cttagggagc
     3601 aggatatccg aaatgaatcc agtgtaattt tagaagaatt aaaaagttat gaagacagcc
     3661 ctgatgatta tgtatacgat gaatacttta aaaatatctt taataaccat ccacttggtc
     3721 gcgatatcat tggaacaaga gagtctgttg caggagtaac tgaaaattca attcaatctt
     3781 attataaaaa acactatgtg ccaggaagaa tggttttatc tattgcgggt gactttcctg
     3841 aggacgagat taagcgcctg actgaaaaat attttagtat aaaaaaagaa agccctttag
     3901 ttccattaga aaacagtcca gttacaaaat cattttccat tcattttata aagagaaaat
     3961 tagaacaagt gaatttcctt ttaggtgcag aaggctttcc gcgtgacttc acaactgccg
     4021 ttcgattggg agtgattgct aatattttag gtgggggaat ggcttctcgt ctttttcaaa
     4081 agattagaga agagaaagga ctgtgttata gtattagctg ttttccttct tcctatttag
     4141 atacaggtat tatgaatata aattgtgcaa cctctaaaga taaattttta aatgcagtag
     4201 aatcaatctt agaggaaatt cgtttattaa aaaaagatgg ttttagtcag gaagaattgg
     4261 attttgctaa gagcaatcaa agaggcggtt tggcaattgg atatgaaatt cctgaaaata
     4321 gaatgactga tattgcaatg caagagcttt atttcggaag attttataat tacgaaaata
     4381 gattagagga acttgcatct gtgacgttag atgaaattaa tgatctagtt ggaaaagttt
     4441 tttcagttga tcaaatgcat tttactggta ttggaaatat tcctaaggta gatatcaaaa
     4501 aaattcatac aaagatctaa aggtttttag tatagggttg agttatcaat ggatgcagta
     4561 aatattaaaa ttcaaaaatt ttttcctgat gtaaaacttc ccgaaaaaaa aacatctgga
     4621 gcagcagcat tggatgtatc tgcttattgt cctaaagcaa atatacaaat tgatgttgga
     4681 caaactgttt taattccaac aggtttttgt ttagaaattc cggaaggtta ttttgtagaa
     4741 atacgtcctc gatctggatt ttctacaaag gaaaacatca tcataccaaa ttctcctgga
     4801 accattgact ccgattatag aggacaagta tttatccctc ttttgaattt gagccagaaa
     4861 ccttttcaaa ttacaaatgg acttagaatt gcccaaatgc tagtggcaaa gtcgatttcg
     4921 attgaatgga atttggtaga tgaaatatca ctcgatacag aaagaggaac gggtggattt
     4981 ggctctaccg gtagacatta aaataacgtg agttcgatgt aaaaacattg aaagattacc
     5041 acctattgcc gcgaacaagc ggcaatggat tttgggtgga aagacgataa gcaccgataa
     5101 aaagatacga tagttcaaaa gcatcaatcg tttccatatc gttttgttat cggtgtcctc
     5161 ggcgatgatt cttaagtcga caacattgag tggagtcgaa gttatctatt cttttaaaga
     5221 ctctaaaact tttctcattt gttttttgtt ttcagtgaga gcttgttttg ttttttcaat
     5281 ttttgtttga tagcccttca atttctcttc tcttttgaca cgaatgtcct caagaaaatg
     5341 ataaatcgct ttcctgtctc tttgatttaa gttttctcct tttttgaaaa aacgcctgtg
     5401 aagagataaa atatcttgtc ttttttctct tttttctaat tttaatttct ctgcgatttc
     5461 aagattttgg agagatttct tttctgtaat caattctatg gcttctgtta ataaaccaat
     5521 ggattttgca gaagaggtaa tgagtgtgtt ccagttttta tgaattttca tataatactg
     5581 ggctgttcta attttaaatg ggaggtttgc ttctacccat gcttcccact ctccatgctt
     5641 tacagtggtt ttcttttctt cgagacgctc tcctatctgt tttgctagga gcaaatggtt
     5701 tcttgcattt tctagaaact taaagtgtaa tacttcaatt tctttaattg tttcgtctct
     5761 atccttggat aattttcctg atgtaactaa ctcgactgaa ttgcttgttt tttttttact
     5821 catactttta tcactcttga ttcataatta caggaatgca aatcttttct ttattacgca
     5881 agcacttgcg acttttaaga gtctgactcc ttataattaa ttactataga gactatcggc
     5941 ataattttag actttgttaa aattctataa gtctttttat tgctttaatc tatttttcag
     6001 gctaataatt ttcatctcat gttttatttt ctgtgattaa ttgaagtctt ttttaggtga
     6061 tttttaaaag aatgtttgaa tcatagtttg taaattacgt attcatttcg gactcaaggg
     6121 atttttaggt taaatataga gaactaagag ttcgagagta agtttaaaaa atcttattat
     6181 caattcagct taaattatca tttcttaaca agtcaaggat tgagttctag gagtcaaagg
     6241 tcaaagaagg tttgctccga ttgccgctga acaataagtt gagcagagtg aaagagtgag
     6301 tattgttggc attttgcatc aatcgcttta caagttctcc atctgcgagt aattgcaggc
     6361 tatcgatttc attagcattc tctaaaattt ttaaatttgc ttatcatctt tattaaatta
     6421 aaaatcttta gacttttaaa tatctttaat tgctgtatcg tatgtcgttc ttgaaatatt
     6481 tgatttttta gattaattgc attcaaggac tgacatcggt tgattctgaa agaaaaacat
     6541 acattcattt gtttcagtaa aatttgcaca agatgctttt tgattctcta tgccttctaa
     6601 agaaagagta atacactcat tgcaacattc atttttataa tttctattta aggaaaaacc
     6661 cttgaaaatt tctttcatgg atagaaagtt tttagggaaa aggttggctt gcgtgtaaga
     6721 atgaataata aataaattca ctccatctgt tgcatagact agatacccga aaatctttct
     6781 tttttgtttg tatcccattg ttttcgccca ataccctttt aggtggtaaa gaggaaattc
     6841 ggcttcttgg tcaaggcttg tataattttc agcccagagc tgtaaattct tcagtgttag
     6901 gatgaatcct tcttgcatat ctcttttttg gtaatttgta gtgtagacct ctaggtggat
     6961 tctgtcttct ttgctgtaag cagtgaataa tggcttcttt aaggaggatg tcttcgcttt
     7021 tctcagttcc cagtgagtag gtaagtcgat acgaaaatta tgagctttaa atgtatatgt
     7081 catctgttct ttactaagag cccaaaaggg aaaggtgatg tagaagatga gtgccactat
     7141 tttcttcata agctaaattg tatcagggtg ctaaaaatta gatagaataa tttttaattt
     7201 ttgtaatttt actttttgac tcatataaat agtaatttta ccttgctatc agatagaaat
     7261 aaatctctaa ccacaagaga gattttatta caggagcatt tagatgacaa aaattgcaat
     7321 taatggtttt ggtagaatcg gtagactcgt tctgagagta ggattagaag atccaaattt
     7381 agaatttgta gcaatcaatg accttgtccc acctgataat ttagcttatt tattcaaata
     7441 tgactctaca catggaaaat acaaaggcac tgttgaacat gatgcagaaa acctcataat
     7501 caatggaaaa aaaattaagt gtttagccga gaaagagcca gaaaaattac catggaaaaa
     7561 tcttggagtt gactatgtaa ttgaatccac tggtagattt acagataaag aaggtgctgg
     7621 aaagcactta acagctggcg caaaaaaagt aatcatctct gctcctgcaa aagataagga
     7681 cattccaact tacgttttag gtgtcaatga atctaaatac aatcctgcta cagatcatat
     7741 tgtatccaat gcatcttgta caacaaattg tcttgctccg ttagtaaaag ttgttttaga
     7801 taattttggt tttgcagaag gtctcatgac tacagtacat gctatgaccg caacacaacc
     7861 aactgtagat ggtccttcaa agaaagactt cagaggagga agaggtgctg ctcaaaatat
     7921 cattccatct tctacaggag ctgcaaaagc agttgggctt tgtattcctg aagttgcagg
     7981 caaactcact ggtatgtctt ttagagttcc aactccagat gtatctgttg tagatttaac
     8041 ctgtaaaact gttaaaccaa cttcactcaa agaaattaaa caaaaaatga aagaagcagc
     8101 tgaagggaat ctaaaaggaa ttttaggtta tacagaagaa gcagttgttt ccaatgactt
     8161 tactagttca actctttcct ctgtatttga tgcagatgcg agtattgaac tcaatgcaaa
     8221 cttcttcaaa ttagtatcat ggtatgacaa tgaaatggga tattccaatc gagttgtcga
     8281 cctaattaga tatatggtaa aaaaaggata agatgaattt accccgaatt gagaatgttg
     8341 acgttaaagg aaaacgtgta tttctacgtg tcgactttaa cgttccttta gaaaatggaa
     8401 aagtttccga caagactcgt atcgagaaaa ctcttcctac aattgagctc ctcctaaaca
     8461 gaggagccaa gttgattgtt gcgagtcatt taggtagacc agatggaaaa gtagattcta
     8521 gatattccat gaagcctgtg caagaagcat ttgaggctat tcttggtaag ccggtaaaat
     8581 tctctgaaca aattgttggt agtgaagtag agaaactatc gcaagaccta aaagatggag
     8641 aagttctatt actagaaaat ctcagatttc acaaagaaga agaagaaaat gatccagctt
     8701 tttcaaaaaa gttagctcaa ttagcagaga tatacataaa cgatgcattt ggtgctgcac
     8761 atagagccca tgcctctaca gaaggaattg cacaccacct tccttcctat gctggaactc
     8821 tcatgtatag ggaaatagaa gtattaacga gtctcatcac aaaaccagaa aagccttttg
     8881 ttgcaattat tggtggagca aaagtttcta gtaaaattaa aatcctaaaa aatctgttta
     8941 acaaagtaga tacaatgttg atcggtggag gaatggctta tacattccta aagtcaagag
     9001 ccgttcctat tggatcttcc ttagcagaaa aagaatttga gccacaggca tttcaaatta
     9061 ttgataaagc agcctatgaa aatgtagatt ttcaacttcc aatcgatcat gtgatcgcag
     9121 attcatttag cgaaaaagcg aagaccaaga cagtcgataa aatgggaatt attgatggtt
     9181 ggatgggaat ggatataggt ccaaaaacaa tttctgccta tgaaaaaata attaaatcag
     9241 ctgggacgat tctctggaat ggtccaatgg gtgttttcga aatggaaaaa ttttccaatg
     9301 gaactatcca aatagctaag gcaatcgcaa aatccaatgc caagtcagtt gtaggcggag
     9361 gggattccat tgcagcaatc aataaagcgg gtgtggaaga taagattact catatctcca
     9421 ctggaggtgg cgcttctcta gaatttttgg aaggtaaaac tttacctgga gtagctgctc
     9481 ttttaaaaaa taaggactaa ttatgcgcaa gaaaatcatg tcaggcaact ggaaaatgaa
     9541 tttaaaccta gcgcaagcaa ctgagcttgc aacaggaatc aaaaaggggt tagaatctaa
     9601 gaaaacctcc ctagatcttt tggtatttcc ttcctcgatt catttagaaa aggtttcaga
     9661 aatactaaaa ggaacatctg ttaaagtagg agcgcagaat atctatccgt ctggactggc
     9721 tgcatttact ggagaaattt ctacagacca attaaaagat cttggaatct cgcatgcgtt
     9781 agttggacac tccgaacgta gacaattttt aggagaaaca gattctttct taaatcaaaa
     9841 aatattattt cttctaaaag cagggtttac tcctgtcttt tgtattgggg aaactctcca
     9901 agaaagagaa gcaaataaaa cgttcgatgt tgtaaaaaga caagtagtag aaggacttaa
     9961 atcagtttct gcatctgata tgaaaaatat tatcattgct tatgagcctg tctgggcaat
    10021 tggaactggt aaagtcgcaa ctccagagca agcagaagaa gtacatgcat ttattcgtaa
    10081 agaactcgct gctttgtatg atacaacagt gagtgattct gtatctattt tatatggtgg
    10141 atcagtaaag cctgataatg ttaaagctct tcttgaaaaa cctaatattg atggtggact
    10201 tgtgggtgga gcaagtcaaa aatgggattc ttaccttgca ttactctaat gagttgacgt
    10261 aggactattc tctaagaaat tggtaacact atgggatttt taatcggaac agtattagta
    10321 ttttttgtta tttcttgtgt attccttatc ttattggtat tggtgcaagc aggaaaaggt
    10381 ggcggtttag gtggtatggt tggaggagca agtcagactg
//
LOCUS       DAOQOS010000162        10395 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_3360_length_10395_cov_6.083656, whole genome shotgun sequence.
ACCESSION   DAOQOS010000162 DAOQOS010000000
VERSION     DAOQOS010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 10395)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10395)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10395
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3360_length_10395_cov_6.083656"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..434)
                     /locus_tag="PLF66_23065"
     CDS             complement(<1..434)
                     /locus_tag="PLF66_23065"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008592765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HMZ66987.1"
                     /translation="MNLKPIHSTREFIDQLKKEKELLVINDPVNPDLEIAEIQRRVVA
                     KRGPAILFTNVIGSKFSVATNLYGSARRIHIAFGSRPVDLIRKIAETAQGIMPPTPKK
                     IWEARGLAFEALKVGLKKESSPRVLESSISPVDIMQVPALRS"
     gene            complement(453..1529)
                     /gene="mutY"
                     /locus_tag="PLF66_23070"
     CDS             complement(453..1529)
                     /gene="mutY"
                     /locus_tag="PLF66_23070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001985769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HMZ66988.1"
                     /translation="MKEQFKLLEWFQKNQRPLPFRKDKDPYKIWISEVMLQQTRVAAM
                     LPSYEKFILRFPNVSSLASATEEEVIQSWKGLGYYSRAIHLRKGAIYLMEMHHGIFPK
                     DLDSVLKIPGVGPYTARAVLSIAYNKNYAVLDGNVKRVLSRYFLFKENIAIPSAHKKL
                     QELADSFLNPNNSGDHNQAMMELGATVCLPAPLCMLCPIQSHCMAFQIGKQAEYPIVA
                     KEKQKLPIQLKFLFLNLNQKILLVKYKKRRFFKTIFTLPFLINGENLSNSYNDLTEFG
                     GYCKSISETGVWKKVGKHTITHHEIELLQMNFSVSGKNEKDLEKILSSSSSSREIEYK
                     WIDKKDIKIEFPSSIAGKLLLDDK"
     gene            complement(1526..2470)
                     /gene="queG"
                     /locus_tag="PLF66_23075"
     CDS             complement(1526..2470)
                     /gene="queG"
                     /locus_tag="PLF66_23075"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HMZ66989.1"
                     /translation="MEPLPKEIENQIQAKAIECGFELFGITKAELPIEDKENLNTWIE
                     KKLYGKMDWYPKNNDLRINLKNLGFTPKSVVVLGVMYQDREYEEVFIKKDYKISRYAI
                     GSDYHKVLRNMAKPFLKFLKEKFPENSFRQGVDSLPVPEKIFAREAGIGWQGKNTNII
                     NENLGSYFFISVILTDLQLLATSPATDRCGTCRACLDACPTGALFEPYKIDAGKCISH
                     NTIEDRSDKMDLESNQWVYGCDICQEVCPWNRIKAKKRDVYTTRDEFKIREEFKVNTI
                     DDFLNMTEEAFQKLVKDSAMDRISYSMFQRNLKAVKEE"
     gene            complement(2477..2968)
                     /locus_tag="PLF66_23080"
     CDS             complement(2477..2968)
                     /locus_tag="PLF66_23080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66990.1"
                     /translation="MKNILVYIILIYLITLNLYSENSILYEKFFLDKLFVYEATLKGK
                     KRIIEKEYKGMNGPSFRVSEEEKKLSLQVNLNNDYFVSSFIQLHCKENACDFISMGKS
                     SLDSELPANQKKNLEKLRDKSLLKVQMVNKKEIVILYTIWPDLKKDFILKEYDNSKES
                     YFD"
     gene            3349..4293
                     /locus_tag="PLF66_23085"
     CDS             3349..4293
                     /locus_tag="PLF66_23085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66991.1"
                     /translation="MDNSEKISQPIVPNTTSFDGDKIKLVNGETLDVQKFTYLLKQKL
                     NDYILKKSTFLIIKSKPPARIGEIRKWKDGKLHRKEFHKWVTEPTKRIKQPNGKEFKL
                     KSLFSKYDWKLNYIYPEFFKNFNVQKSMTKFKEHLLKHPEYKNHLGRLSILEALAIYH
                     YSLKWGVRPLNTDLENNTLSEYTKQYEKILSTALDKLPRYTKRTPVYHGTTLRESQIL
                     DLKLKVKNGEPFKFGFFTSVELRIVDGEKYMTKNRGRIKKDERLVMFVIHAKTAKDVT
                     NLRWRTDTHEAIIGHSAEYEVFDVSLKRNYTEIKLREI"
     gene            4290..4505
                     /locus_tag="PLF66_23090"
     CDS             4290..4505
                     /locus_tag="PLF66_23090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66992.1"
                     /translation="MNKKIHINKYKEQPKKEFTPLSEKERQQLIKQMDEAMEKDRTEY
                     AKWWKTLTPEQQKEHDDDVSRLCDVEL"
     gene            4896..5564
                     /locus_tag="PLF66_23095"
     CDS             4896..5564
                     /locus_tag="PLF66_23095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66993.1"
                     /translation="MKTFFPNNLTKLFFVTIILLFSNCFSFKESEIDFAIQDPKPAKE
                     KKNLQVWIKMTQLPEYNTNLPLENRNPFITFKFYKFIRQSKQFESVNILYSKETKLPN
                     NAIVFDIQFSSLLEEEKFHSLYFPFGILGLGLTRYFISSNYGFLLFTGLGGPDTVHKL
                     DYKGNLVAYNSNLQEIARASLDYKSEFNSNMLYYGKEKESNLERKDLFEKAIASILKQ
                     LEGK"
     gene            5561..6157
                     /locus_tag="PLF66_23100"
     CDS             5561..6157
                     /locus_tag="PLF66_23100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66994.1"
                     /translation="MKFFLFLILFFSHCIVIQKTSPLQNFPKAGSIAQNQNSVFIISN
                     HEVYKNGIKVKSEATHYHLVDSVRNSGIFSKIETDIEKADQIIEIEIQTHREDNAYLP
                     FITTLTLSLVPYFQRIEMKENFRLVKKNGDVLYEANRSQKFNYWFGFFFFINGIIQLT
                     EEPINNHPGFELKLAEDINQNIVDEMAIKNLLPKRETK"
     gene            6315..9413
                     /locus_tag="PLF66_23105"
     CDS             6315..9413
                     /locus_tag="PLF66_23105"
                     /inference="COORDINATES: protein motif:HMM:NF020076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT domain-containing protein"
                     /protein_id="HMZ66995.1"
                     /translation="MKTFKKFQITFLIILTFSFLFSGKELEDADNPDSPLSLTAGDGA
                     GLVLKNYESNTVLDGFIAFTEIKLKFYNPENRQREGRFRITLPDNAHLARFAMMIDGK
                     WQEGEVVEKEKAVRAYEDFLHRKQDPALLESDAGNEFSARIFPIPAKGDKDLIISYST
                     VLDTNPPHYNVPLVGLPEIENFRVNVFYDEKEFKQEKVADNSKEEGSTVNRKVFTIQK
                     KNYKPERDYAFEHKTKGNLSLQNKNLFALHFVPFKTSSNEKFKDDRAIFLIDTSASSA
                     VRYKEHLERIEELLRQFPSKEIHIFGFDNKLTYYGIGKESLDKVKKVLPLGASNLYSA
                     IKGISAKIENKTGRLILVSDAVPTAGIIEKTKIAELIKSQTWISRLDVLVPGTYKDPG
                     IIKKIVPLGKNAGTSIDLGDDIEEILNKLERKVYTENKISIPGAKWYYPDQIDSIQSG
                     DPVVVFGELKEANTNPSKEIKFNGNSIENFTSLTMDELLLEREAMAARINRLIENSEK
                     EQNEDTAKGLELQAKELSIKHRIQCQYTSFLVLETEADYARFQITRNSLTNILTIGVG
                     GLEVINRRNSKLYEFLDEEKIEEAKRAKQEADRLAKNDLKKKGKKKNGNISANGASDG
                     FTSADESSSSFKDMDDEISPKKMSEAPSSSIQNEKADKSTIADNKPSPPPATKTEEAK
                     KELSLDKPRPIPAKDISPQIEIPERERRKLESIRRPPPQPRPEPAEKREKVEPYTGNM
                     KKFYSLLSKKEFKKAEQFALDWRTKSADDALAIVALGDAYEKQGNKNEAVRAYTSLID
                     YFPKRADIRRWAGEKLMSIGEYDDSIDTLKHALDQRPDHPSSYHLLAIAYIKDKQYKS
                     AATIALKGINFQFDGRFGSVHDILYDDLDLAYSLAMKTDSKDREYFNKIKTEYKVKQL
                     TKEIRFILVWETDANDVDFHIYDKEGNHAFYSSKQLSTGGELYADLTGGYGPECFRIL
                     NPYAFPYKLEAHYYSRGPMGYGMGAVQIIRYDGEKDLEVETRDYVIMNDGAFLDLGTV
                     KE"
     gene            9754..>10395
                     /locus_tag="PLF66_23110"
     CDS             9754..>10395
                     /locus_tag="PLF66_23110"
                     /inference="COORDINATES: protein motif:HMM:NF024571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HMZ66996.1"
                     /translation="MIYGPRRTGKTSFVENFLTTFSGKYYHGVGDDSDLVSIFSNPSI
                     SKFISLFGGYDLLFFDEAQKLPNVGTVLKILIDHLPKTQIIASGSSSFELSSQVGEPL
                     TGRYTTLFLYPMSVIELKNQFGGMYVYQNLETLLLYGSYPESIQSINLETKREYLINL
                     RNSYLFKDILEMERLKNADKLADILRLLAFQIGKEVWLNELSLNIGVSKQTIEK"
BASE COUNT         3630 a         1789 c         1714 g         3262 t
ORIGIN      
        1 caggagcgaa gagcaggaac ttgcattata tcgacaggcg aaatggagga ttcgagaact
       61 ctaggagaag attctttttt taaaccaact tttaacgctt caaaagcaag tcccctcgct
      121 tcccaaattt tcttgggtgt tggtggcata attccttgtg cagtctctgc aatctttcga
      181 attagatcta ctggtctaga tccaaatgca atatgaatcc tccgagctga tccatagagg
      241 ttagttgcaa cggaaaactt agaaccaatt acatttgtaa aaagaatagc aggacctctt
      301 ttcgcaacaa cccttcgttg tatttctgct atttctaaat ccggattgac tggatcatta
      361 attactaata attctttttc tttttttaat tgatcaatga attctcttgt agaatgaatc
      421 ggttttaagt tcattttatt tctcgtatta ttttatttat catctaacag tagtttaccg
      481 gctattgaag aaggaaattc tattttaata tcctttttat ctatccactt atattcaatt
      541 tctctagagc tagaagaaga tgaaagaatt ttttctaggt ctttttcatt tttgccagat
      601 acggaaaaat tcatttgtaa aagctcaatc tcatgatgag taatggtatg ctttccaact
      661 tttttccaaa ctccagtctc ggatatactt ttacaatacc ctccaaattc tgtaaggtca
      721 ttgtatgaat tagaaagatt ctctccattg attaaaaatg gcaatgtaaa gatagtctta
      781 aaaaacctac gttttttata ttttactaaa agtattttct gatttaaatt tagaaataga
      841 aactttaatt gaataggaag tttctgtttt tctttagcaa ctatcggata ctctgcctgt
      901 tttccaattt gaaatgccat acagtgagat tgaatcggac agagcataca aagaggagcc
      961 ggcaaacaaa cagttgcccc taattccatc atagcttggt tatgatcacc cgaattgttt
     1021 ggatttaaaa atgaatctgc gagctcttgc aattttttat gagcagacgg gattgcaata
     1081 ttttccttaa atagaaaata ccgagagaga acccttttta cgttcccatc caatacggca
     1141 taattcttgt tataggcaat cgataatact gctcttgccg tataaggacc cacaccagga
     1201 atttttaaaa cagaatccaa atcttttggg aaaatcccat gatgcatctc cattaagtaa
     1261 atcgcaccct tccttaaatg aatcgctcta gaataatatc ctagtccttt ccaagattga
     1321 attacttctt cctctgttgc acttgcaaga gacgaaacgt taggaaatcg aagaataaat
     1381 ttttcataag aaggaagcat tgccgcaact cttgtttgtt gcaacatgac ttcgctaatc
     1441 catatcttat agggatcttt gtcttttcga aatggaagag gtctttggtt tttttgaaac
     1501 cactcgagta acttgaactg ttctttcatt cttctttcac tgcttttaga tttctttgaa
     1561 acatagagta agagattcga tccattgcac tgtctttcac taatttttga aaagcctctt
     1621 ctgtcatgtt taaaaaatca tcgattgtat ttactttaaa ttcttctctg attttaaatt
     1681 catccctcgt ggtataaacg tctctctttt ttgctttgat tcgattccaa ggacaaacct
     1741 cttgacagat atcgcaacca tacacccatt gattgctttc taaatccatt ttgtcgcttc
     1801 tgtcttcaat cgtattatga gaaatgcatt tacctgcatc tattttgtat ggctcaaaga
     1861 gagcacccgt tggacaagca tctaaacagg ctctacaagt tccacatcta tccgtagcag
     1921 gacttgtggc taatagctgt aagtcagtaa gaatgacact gataaaaaaa taagagccta
     1981 gattttcatt gatgatattt gtattttttc cttgccaccc gattcccgct tctctcgcga
     2041 aaattttttc tggaacaggt aaagaatcaa ctccttgtcg aaacgaattt tctggaaatt
     2101 tttctttcaa gaatttcaga aaaggctttg ccatatttcg taaaaccttg tgatagtcag
     2161 atcctatcgc ataacgtgag attttataat ctttttttat aaagacttct tcgtattctc
     2221 ggtcttgata catcactcct aaaacgacaa cagatttggg agtaaatcca agattcttta
     2281 aattgatccg taaatcattg ttctttggat accaatccat tttcccatat aatttttttt
     2341 caatccaagt atttagattt tctttgtctt ctatcggaag ttctgctttt gtgataccaa
     2401 aaagctcgaa accacattcg atcgcttttg cttgtatttg attttctatt tcctttggta
     2461 gtggttccat ttcacttcaa tcaaaataac tttctttgga attgtcgtat tcttttaaga
     2521 taaaatcttt tttcaaatca ggccagattg tatatagaat cactatttcc ttcttattta
     2581 ccatttgcac tttaagtaaa cttttatcgc gaagtttttc taagtttttt ttctgatttg
     2641 caggaagttc actgtccaat gaactttttc ccatcgaaat aaaatcgcaa gcattttcct
     2701 tgcaatgcaa ttgaatgaaa ctcgaaacga aatagtcatt atttaagtta acctgtaaag
     2761 ataacttctt ttcttcttca gagactctaa aactgggacc attcatacct ttatactctt
     2821 tttcaataat tcttttcttc cctttaagag ttgcctcata tacaaaaagt ttatcaagaa
     2881 aaaacttttc ataaagaatt gagttctcag aatatagatt cagcgttatg aggtatatta
     2941 aaataatata aactaaaata tttttcattt gggttctcca gcaatctttt taaatattct
     3001 ctatcttatc taggaattat tttttcatag atactcagaa tctcaagctt cataattcca
     3061 attttgattc gatctgggtt tctttttcaa tcgttattac taatataaaa aaattataat
     3121 gagttgaatt gtcaaccaaa tcatgagtag tttccgtagt taaaacgtta tgcatctaat
     3181 catttaatac aattattcct ctttaactcc catttcctta agctactagc acattctaga
     3241 tagcaaataa ttcttaaaaa aggctaaatt tcatttgccc gaataaggac tttttcaaga
     3301 gttggtaaat ttgttaaccc gaaatttctt ttagaataga ataaaggaat ggataactca
     3361 gaaaaaattt cacaaccaat tgttccgaac acaacttcct tcgatggaga taaaataaag
     3421 ttagttaatg gagaaacgct tgatgttcaa aaatttacat accttttaaa acaaaaatta
     3481 aatgattaca tccttaaaaa atctacattt ctaatcatta aatctaagcc tcctgctagg
     3541 attggagaaa taagaaaatg gaaagatgga aagctccatc ggaaagaatt tcataaatgg
     3601 gtaactgaac ccacaaaacg tattaaacaa ccgaatggga aagaatttaa actgaaaagt
     3661 ttattttcta agtatgactg gaaactaaac tatatttacc ctgaattttt taagaacttt
     3721 aatgtgcaaa agtctatgac aaaattcaaa gaacatttat taaaacatcc cgaatataaa
     3781 aaccatcttg gaagactttc tattcttgaa gcattggcta tttatcatta ttctctaaaa
     3841 tggggagtta gacctttgaa tacagattta gaaaataata ctctatctga atacacaaaa
     3901 cagtatgaaa aaattctttc gacagcattg gataaacttc ccagatacac gaagagaaca
     3961 cccgtttatc atggaactac tttacgtgag agtcagattt tggatttaaa acttaaagtt
     4021 aaaaatggag aaccatttaa atttgggttc tttacttctg tagagcttag gatcgtagat
     4081 ggggaaaaat atatgactaa aaacagaggg aggattaaaa aagatgaaag attggtcatg
     4141 tttgttattc atgcaaagac agcaaaagat gttacgaatc taagatggag aacggatact
     4201 catgaggcta tcattgggca tagtgcagag tatgaagtgt ttgacgtttc attaaagaga
     4261 aattatactg aaatcaagtt gagggaaata tgaataaaaa aatacatatt aataaataca
     4321 aggaacaacc aaaaaaagaa ttcactcctt tgtcggaaaa agaaagacaa caacttatta
     4381 aacaaatgga cgaggctatg gaaaaagaca gaaccgaata tgccaaatgg tggaaaacgc
     4441 taacaccaga acaacaaaaa gaacatgatg atgatgtctc tagactttgt gatgttgaat
     4501 tgtgactaat aggttaacgg ctaatttcga ttagtaattt ccctttctat aaaagcaaac
     4561 gcattctttt catcctttgc aattaagaag tctgagtaat cagagcctga acagaattgt
     4621 cagaaattat ttctaaaaca aatgaaatga cttagagtct ttaaagaaaa actaaaaatt
     4681 aaaacttgta gagaaatagg ctccttttaa cttgaattca attttaaaat ctcaccttgc
     4741 gcaaggtgag ctaggcgttc aaatgaggtt tgctcctagc ggagggcttg ccgccgcagg
     4801 ctattgattt tattagttta ttagtattca ctaatatttt caaaatcatc ttttgttcct
     4861 caaattatta attttataga atgcgagttt atattatgaa aacttttttt ccaaataacc
     4921 taactaaatt attcttcgtt actataattt tacttttttc aaattgtttt tcttttaaag
     4981 aaagtgaaat tgattttgca atccaagacc caaagcctgc aaaagaaaag aaaaacttac
     5041 aagtatggat caaaatgact cagcttccgg agtataacac caatttgccg cttgagaatc
     5101 gaaatccatt catcaccttt aaattttata aattcattcg acagtccaaa cagtttgaat
     5161 cagtcaatat actttactct aaagaaacaa aacttcctaa caacgcaatt gtatttgata
     5221 ttcaattcag ctcactcttg gaagaagaaa aattccactc tctctatttt ccttttggaa
     5281 ttctaggttt aggtctcaca cgttatttta taagtagcaa ttatggattt cttttattta
     5341 caggtcttgg tggtcccgat acggttcaca aattggatta caaaggaaac ctagttgcat
     5401 acaattccaa tctgcaagaa atcgctagag caagcctcga ctataaatcc gaatttaata
     5461 gcaatatgtt gtattatgga aaagaaaaag aatcaaatct agaacgaaaa gatttatttg
     5521 aaaaagctat tgcatcgatt cttaaacaat tggagggaaa atgaaatttt ttttatttct
     5581 aattctattc ttttcacact gtattgtaat tcaaaagaca tctcctctac aaaattttcc
     5641 taaagcaggg agcattgcac aaaaccagaa ttctgttttt attatatcca atcatgaagt
     5701 ctataagaat ggaatcaaag taaaatcaga agctactcat tatcatctag tagattccgt
     5761 tcgaaatagt gggatctttt caaaaattga aactgatatt gaaaaggcag atcaaatcat
     5821 tgaaattgaa attcaaaccc atagagagga taatgcgtat ttacctttta tcactacact
     5881 tacactttct ctagttccgt attttcagag aattgaaatg aaagaaaact ttcgtttggt
     5941 taaaaagaat ggagatgttt tatatgaagc aaatcgaagt caaaagttca attattggtt
     6001 tggttttttc ttttttatca atggaatcat acaactcacc gaggaaccga tcaacaatca
     6061 tcctggattc gaattgaaat tagcagaaga tataaatcag aacatagtag atgagatggc
     6121 aataaaaaat ttattgccta aaagagaaac aaaatagatt ctatcattcg acccatcctg
     6181 tcaaaagaag gattattcat cgatagcaaa gcgatgaata atcacatata tcggtgtgaa
     6241 tctgtatgta tcagtggtag ccttttttcc tctgtcttat attgacagta aaaataatct
     6301 cagggagaaa ttacatgaaa acttttaaaa agtttcaaat tactttttta ataatactca
     6361 ccttcagttt ccttttttcc ggaaaagaac tagaagatgc ggataatcca gattcgcccc
     6421 tttccttaac tgctggggat ggagctgggc tagtcttaaa aaactatgag tcaaatacag
     6481 ttctagatgg atttattgcc tttaccgaaa tcaaattaaa gttttacaat ccagaaaata
     6541 ggcaaagaga aggaagattt aggataactt tgccggataa cgctcatttg gcacgatttg
     6601 caatgatgat cgacgggaaa tggcaagaag gagaagtggt cgaaaaagaa aaagctgttc
     6661 gtgcctatga agacttttta cacagaaagc aagatccagc tcttttagaa tcggatgcag
     6721 gaaatgaatt tagtgcgaga atctttccaa ttcccgcaaa aggagacaaa gacttaatca
     6781 tatcttactc aacggttcta gatacaaacc ctcctcatta caatgttcca ctcgttgggc
     6841 ttccagaaat tgaaaacttt agagtaaatg tattctatga tgaaaaagaa tttaagcaag
     6901 aaaaagtagc agataattct aaagaagaag gatctaccgt taaccgaaaa gtatttacaa
     6961 ttcaaaaaaa gaattataag cctgagagag actatgcatt tgaacacaag acaaaaggaa
     7021 atttaagtct tcaaaataaa aatcttttcg cacttcactt tgttcctttt aaaacttctt
     7081 ccaatgaaaa attcaaagat gacagggcta tatttttaat agatacctct gcttcctctg
     7141 ctgttcgtta taaagagcat ctagagagaa tagaagaatt actccgccaa tttccatcca
     7201 aagaaattca tatctttgga tttgataata aacttaccta ctatggaatt ggaaaagaga
     7261 gtctagataa agtaaaaaaa gttttaccgt taggtgcaag taatctctac tctgcaatca
     7321 aaggaatttc tgctaaaatt gaaaacaaaa ctggaagact cattttagtt tcagatgcag
     7381 ttcctaccgc cggaatcatc gaaaaaacaa aaatcgcaga acttatcaag agtcaaacct
     7441 ggattagccg cttggatgta ttggttcctg gaacttataa agatccaggg attattaaaa
     7501 aaatcgtgcc gctcggaaaa aatgcgggaa ctagcattga tttaggggac gacatagaag
     7561 aaattttaaa taaactggaa agaaaagttt atacagaaaa taaaatttcg attccagggg
     7621 caaaatggta ttatccggat caaatcgact caattcaatc tggagatcca gtggttgtat
     7681 ttggagaatt aaaagaggca aatacaaatc cttctaaaga aattaaattc aatggaaaca
     7741 gtatcgaaaa ttttacaagc ctcaccatgg atgaactcct actcgaaaga gaagcgatgg
     7801 cggcaaggat caatcgtctg attgaaaatt ctgaaaaaga acaaaatgag gacactgcaa
     7861 aaggactcga attacaagca aaagaattgt ctattaaaca tagaattcaa tgccagtata
     7921 cttctttctt ggttttagaa acagaagcag attatgcaag atttcaaatc acaagaaatt
     7981 cacttacaaa tattttgacg ataggcgtag gaggtctaga ggtaatcaac cgtcgcaact
     8041 ctaaactcta tgaattttta gatgaagaaa aaatagaaga agcaaaaaga gccaagcaag
     8101 aagcagatcg attagccaaa aatgatctaa agaaaaaagg aaagaaaaaa aacggaaaca
     8161 tttctgcaaa cggagcctcc gatggattta cctctgctga tgaatcgagt agtagcttta
     8221 aagatatgga tgatgaaatt tctccaaaga aaatgtctga ggctccatcg agctctatac
     8281 aaaatgagaa agcagataaa agcacaattg cggataacaa accctctcct ccgcctgcca
     8341 caaaaacaga ggaagcaaaa aaagaattga gtctagataa accaaggcca attcctgcaa
     8401 aagatatatc tccccaaatc gaaattccag aaagagaaag aagaaaattg gaatccattc
     8461 gaagacctcc cccacagcct agaccggagc cagcggagaa gagagaaaaa gtagaacctt
     8521 atactggaaa tatgaaaaaa ttctattctc ttctttctaa aaaagagttt aaaaaagcag
     8581 aacagtttgc actcgattgg agaaccaaaa gcgctgatga tgcacttgcg atagtcgcac
     8641 taggagatgc ttacgagaaa caaggaaata aaaatgaagc agttcgagct tatacttctc
     8701 tcatagacta tttcccgaaa cgggcggata ttagacgttg ggcgggagag aaactcatgt
     8761 cgattggaga gtatgatgac tccattgata ctttaaaaca tgcactcgac cagagaccag
     8821 atcacccaag tagctatcat cttcttgcaa tcgcctacat caaagacaaa caatacaagt
     8881 ctgcggcgac tattgcacta aaaggaatta attttcaatt cgatggaagg tttggttctg
     8941 ttcatgatat tttatatgat gacttagatc ttgcatacag tcttgcaatg aaaacagata
     9001 gtaaagacag agagtatttt aataaaatca aaacagaata taaagtaaaa caactcacaa
     9061 aggaaatccg atttattcta gtttgggaaa ctgacgcaaa cgatgttgac tttcacatct
     9121 atgataaaga aggaaaccat gctttttaca gctctaaaca attgtcaaca ggcggagagc
     9181 tttatgccga cctgacaggt ggttatggtc ctgaatgctt tcgaatttta aacccttacg
     9241 ctttccctta taaattggaa gcgcattatt acagtcgcgg tcctatggga tatgggatgg
     9301 gtgccgtcca gatcattcgc tacgatgggg aaaaagattt agaagtagaa accagagatt
     9361 atgtaattat gaacgatgga gccttcctag atctgggaac tgtaaaagag taaatttaac
     9421 ttagaccatt ttgttaaaat gtcatccttg tagatcaagg atgactacga gactgtgttt
     9481 aagctttaaa aaaaatcaaa tcacaaagag cacgaagatg atcctcagct ttactccctc
     9541 cttttctgtg gcgctacttt tactcagttc catcgaattt taactaaatt tagttccttt
     9601 tacaagttta aagagaatag ttaccatatt tatataaatt tggctattct agttgccata
     9661 tttataaaat tttattaact ttacttcatg gattggttca atcgctactc cttaggagaa
     9721 ttcgtaagta aattagagca atgaaaggtt ctcttgattt atggtccgag gagaactgga
     9781 aagacatctt ttgtagaaaa ttttttaaca accttttcgg gaaaatacta tcatggcgtg
     9841 ggagatgact ctgacttagt ttcgattttt tcgaatccat ctatttccaa attcatttcc
     9901 ctcttcggag gttatgattt attatttttt gatgaagcac aaaagcttcc gaatgtagga
     9961 accgttttaa aaatcctaat cgatcatctc ccaaagactc agatcattgc aagtggctct
    10021 tcttcctttg agttatcctc tcaggtagga gaacctctca ctggacgata tacgacactt
    10081 tttctctatc caatgtctgt tatagaatta aaaaaccaat tcggtggtat gtatgtttac
    10141 caaaacttag aaactttact cctttacggg tcctatccag aatcaattca atctatcaat
    10201 ttagaaacga aaagagaata tcttatcaat cttagaaact cttatctatt taaagatatt
    10261 ttagaaatgg agagactaaa aaatgcagat aagttagccg acatactaag actacttgct
    10321 tttcaaattg gaaaagaagt ctggttaaac gaactgagtt taaatattgg tgtatcaaaa
    10381 caaaccatag aaaaa
//
LOCUS       DAOQOS010000163        10264 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_3432_length_10264_cov_7.598100, whole genome shotgun sequence.
ACCESSION   DAOQOS010000163 DAOQOS010000000
VERSION     DAOQOS010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 10264)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10264)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10264
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3432_length_10264_cov_7.598100"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            273..686
                     /locus_tag="PLF66_23115"
     CDS             273..686
                     /locus_tag="PLF66_23115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004785373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HMZ66997.1"
                     /translation="MSPKMEKDIQSYHKKLSAVDKDICDLLYQEISKNLPNAERKLWH
                     SHPVWFFEGNPIVGYSKLKSCVRLMFWSGASFGEEKLKLGSGKFKDASFEYTSADQIK
                     KTELKRWLKKSEKIQWDYKNIVKRKGVLVPLKGIE"
     gene            complement(714..1640)
                     /locus_tag="PLF66_23120"
     CDS             complement(714..1640)
                     /locus_tag="PLF66_23120"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HMZ66998.1"
                     /translation="MEHIDYFTEQFQFLFHIAYRMLGSLNDAEDVMQDCFIEFSKIPL
                     EKMKSPKKLLTTILSRTAIDRYKKAKREREIYKGVWLPEPIVAEAKTNPELLFEKKES
                     ISIALLFLLERLNPKERAAFVLRDLFDLEYQEIAEIFHWTNENTRKLVSRARSAIASE
                     NSKYSVSKSELETILLHFMKAIQKGNKDSLIQLLESDICLYSDGGGKVRAALKPIFGT
                     KKVSQFLIGLSRKPKAKKMKPIFILVNSKPALAFFLNKEIHSILFLEPNEKKLKKIFI
                     WLNPDKINSLKITNPSKFDLAHIAWKLFRLKK"
     gene            complement(1641..2174)
                     /locus_tag="PLF66_23125"
     CDS             complement(1641..2174)
                     /locus_tag="PLF66_23125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ66999.1"
                     /translation="MESLILKPIDTPKGIFLKILYWYSKISFGKVIAPIKIIYSRAPK
                     LIAVAQSLEKAEKSLSLSSKLVSLIKAFVSPKNECAFCNDIAIAMAVKKKIGKDLFKD
                     LEFFSESPHYLESEKIALQYISEILENKRASDSTIEKMKLHFNEKEIVEITWVYAMEV
                     YYNSMGKPFGLTSDGLS"
     gene            2434..4269
                     /locus_tag="PLF66_23130"
     CDS             2434..4269
                     /locus_tag="PLF66_23130"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HMZ67000.1"
                     /translation="MKKFYSKYFLIFFSFSILYSGCNYSKENICDPNSPIAKLYLVWS
                     ASNNFLIPYCGIRLVTLPAPVLSNLKNNSIVETGFLIGTAPAGTSSVEISLDNGSFQT
                     ATGTTNWTYKLPTGTSTWRYGTKHRITVRSNTLVSGMNMVSETSSITVRKGHNKDING
                     DGYADLVVSAFQYPSGGLQGRVYIFYSRGSSGIITTTTQTGTVSNAVILTGELSSAFG
                     RSVIKADINSDGYADLVIGATNLNTSTGRVYIFLSPGSAGIAATSTPQTSANSVITGE
                     VTNSNFGSGVTVGDFNLDGYPDLVVNAFSFSTATGRLYIYYSSANGISSRTASVATDT
                     TITGETTNNVFGYVFESTGDINGDGYPDLVTAAYVYNSKQGRAYIFYSQGSAGITGTL
                     SASSPPAGTTVISGEPGIEGNFGVSITVGDYNGDGFSDVVIGANYVDTSRQGRVYVFH
                     SAGKSGVTTTAAASATTILTGETGTTNSFGAYCVSLDGNLDGYDDLVCGASAASSGAL
                     NKIYYFQSSSNGIATGFANLVSNIIITGENSSDQFGRFLATGDFNGDGFSDIAVTALT
                     LNSSIGKSYIFQNNRTNGFTSVSASGATSSQVGENTSDRYGEFVQ"
     gene            4282..5604
                     /locus_tag="PLF66_23135"
     CDS             4282..5604
                     /locus_tag="PLF66_23135"
                     /inference="COORDINATES: protein motif:HMM:NF040144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5683 domain-containing protein"
                     /protein_id="HMZ67001.1"
                     /translation="MKYIKYLLLVILLSTLTLFADEVTLRNEKVIEKVKVKISVDKVA
                     VTYLDNKTETYPKKEVKSIRIKPIIINNPVTEKDKAENEKEKIRAAESLQNLTGLEIQ
                     ENQKLKVAVLNFKTSANISKEEADIIVETITVELVKTKLFILVDPILVKQVMDEKGGV
                     GCSDNPANCKMPAAEIAKSFQVSKIVTGTINKIKNSYYVSGNVIDTNSKNIDFAESSI
                     ATSFEKIHDTSENFSKKVAGGILSISKTEINLNQESKGFFSNLFKSNSESKADNISQD
                     SPSKMRYVWRSALLPGWGQIANDRKLKGFSLLGLFIVGVGFEISSYKNYKSAIEDYRS
                     AGDNFILNTSLTNANVAGFAFYLNSRNINSQRDQIDLYEHRLESGAGFLALLYLYNLF
                     DAYYFAKDPAKSALDSSREITPKFAAIPTRNSVGQTYTQYNFSIEMRF"
     gene            complement(5808..6068)
                     /locus_tag="PLF66_23140"
     CDS             complement(5808..6068)
                     /locus_tag="PLF66_23140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system HicB family antitoxin"
                     /protein_id="HMZ67002.1"
                     /translation="MSKKNVLTVRIPEDLKDRLERTASAQGVSLNQFALYAFTRGLND
                     VETAQFFKKRIASKTKESLEKNFDKVMKKVKARKTSPEWDKI"
     gene            complement(6061..6498)
                     /locus_tag="PLF66_23145"
     CDS             complement(6061..6498)
                     /locus_tag="PLF66_23145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HMZ67003.1"
                     /translation="MRITLDTNVIYQALRDSGGASNCIVRLVRERKLELCLSIPVFME
                     YSDVLLREKSLKDLELSKKDIKDFLDYICSVASPFSISFSMRPNLGDEADNMFIELAF
                     ASNSNFLITSNTRDYTIGNNLLFDSFKVITPTDFARMWRKFYV"
     gene            6776..8017
                     /locus_tag="PLF66_23150"
     CDS             6776..8017
                     /locus_tag="PLF66_23150"
                     /inference="COORDINATES: protein motif:HMM:NF024571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HMZ67004.1"
                     /translation="MNFEISDFSFLKRDIYVDRFLPFLDKNIIKILTGHRRVGKSYML
                     FQIIEMLKEKGVKPEQIIYINKELAEFASIKNHKDLLSYVEKFRKKKKQKIYLFIDEV
                     QEIEKFETALRSLLAENIYDIYCTGSNANILSGELATNLAGRYIEFKIYSLSYPEFLN
                     FHKLKNNKDSLMQYFKFGGLPFLIHLDLKEEIVSEYMRNIYNTILLKDVVARYQIRNV
                     AFLERLLEYLADNTGSLVSAKSISDFLKSQRINISPNVILNYISYLESAFIIFKVQRR
                     EVGGRKIFEIGEKYYFEEIGIRRTLVNSNISDIHKVLENIIYLHLCIAGYEVRIGKLD
                     NKEIDFVCSRKEEKLYIQVAYLIADEKTFEREFGNLLLIKDNFPKYVVSMDESIEGNY
                     KGIKHIHIQNFLTKVLQDKLP"
     gene            8165..8515
                     /locus_tag="PLF66_23155"
     CDS             8165..8515
                     /locus_tag="PLF66_23155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67005.1"
                     /translation="MYHTFIFYSFFLVFLAAIANLYRTSSRIVSHFYITSEWLKAKKV
                     EIISKITRLIDYSVLLELEKILAISEQQTNEWDSLTALDKAKIEKGLDSIQKGKVISH
                     SMVKEKIDQFIKEL"
     gene            8762..8908
                     /locus_tag="PLF66_23160"
     CDS             8762..8908
                     /locus_tag="PLF66_23160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67006.1"
                     /translation="MSLYFIIMATIGLICGVYLIFSGMAGIISKRVADDVTNRLKSEM
                     KHGE"
     gene            complement(9321..>10264)
                     /locus_tag="PLF66_23165"
     CDS             complement(9321..>10264)
                     /locus_tag="PLF66_23165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67007.1"
                     /translation="LISHISYLISLAFLYIYVLSTPGGIYWDVVTNYRLAHFNIDINK
                     FEKLLRLISESDQKKITIPKLLPNEMINIIGNESVDIFPYQVGRVLASGLYWKNRPVY
                     QSYITSSEWLIQQNVNFFNDHEKSPEYIIFHYPYSLDHRYLLNDELQTFLRIRDLYES
                     ILLKDNFLLLKKRGNKSRSANWETKLKQISKPNATIIFENEIIQKSSIGNNICVGSLD
                     IRKSLYGILKKILYKEEPIFITYYLDDSSRTFRYIPDLGKSGILLYPFLESSGDLYDF
                     FSKKERVFPVVTGISIDFRTNESFYEENYKIEIKCTN"
BASE COUNT         3432 a         1542 c         1725 g         3565 t
ORIGIN      
        1 ttaaaattat attctcttga taaaagtaac ttagcagcct gataggttac gaaaattcta
       61 atgtattaat aaaatcaata gccattaata tctttccttc ccatttatac gagaagtgaa
      121 taaatagttg gtttggtttt attatgatta aactaagaat agagaaaggc attctctaca
      181 ttgttttgat ttgagaagat tatttatact ttctaaatac aaaaaatatt tatatcttgc
      241 tagaaattag tctatcctac tttttaggtc ttatgagccc aaaaatggaa aaagacattc
      301 aatcctatca taaaaagctt tctgcagttg ataaagatat ttgcgatctt ttgtatcaag
      361 aaatttctaa gaatttaccc aatgcagaac gtaagctttg gcactcccat cctgtttggt
      421 tttttgaagg caacccaatt gtcggttata gcaaacttaa atcctgtgtt cgattgatgt
      481 tttggagtgg agcaagcttc ggcgaggaaa aactaaaatt aggttcgggt aaatttaaag
      541 atgcatcttt tgaatatacc tctgccgatc aaattaagaa aacggaattg aaacgatggc
      601 taaaaaagtc agaaaagatt caatgggatt ataaaaacat tgtaaaaaga aaaggtgtcc
      661 ttgtcccatt gaaagggata gagtaggagt agattctatt accttaattt attctacttc
      721 ttcaatcgaa ataatttcca tgcaatatgc gctaagtcaa acttggatgg atttgtaatt
      781 tttagagaat tgattttatc tggatttaac caaataaata ttttctttaa cttcttttca
      841 tttggctcta aaaataaaat agaatggatt tctttattta gaaaaaatgc tagggctggt
      901 tttgagttta ctaaaataaa aattggcttc atctttttag cttttggttt tctggataat
      961 ccaattaaaa attgagaaac ttttttcgtc ccaaatatgg gttttaaggc agctcttact
     1021 tttccaccgc catctgaata aaggcatata tcagattcta ataattgaat aagagaatct
     1081 ttatttcctt tttgaattgc tttcataaaa tgaagtaaaa tagtttctaa ttctgattta
     1141 cttaccgaat actttgaatt ctcagaagcg atagccgaac gagctcttga tactagttta
     1201 cgagtatttt catttgtcca atgaaatatt tctgcaatct cttggtattc taggtcaaat
     1261 aaatctcgta atacaaacgc ggctctttct ttaggattca atctttctag caaaaataaa
     1321 agtgcaatag agatagattc tttcttttca aaaagaagtt ctgggttcgt ttttgcttct
     1381 gctactatcg gttcaggaag ccacacgcct ttgtatatct ctctttctcg ttttgctttt
     1441 ttgtatcggt ctatcgcagt tctacttaga atagttgtaa gtaatttctt aggagatttc
     1501 attttctcca gtggaatttt tgaaaactct ataaaacagt cttgcattac atcttcagca
     1561 tcattcaaac ttcccagcat acgataagcg atatgaaata aaaactgaaa ttgttctgta
     1621 aagtagtcaa tgtgttccat ttatgacaga ccatccgatg tgagcccaaa aggttttccc
     1681 attgaattgt agtaaacttc catcgcatac acccaagtaa tctctacgat ttctttttca
     1741 ttaaaatgta atttcatttt ttcgatggta gaatcacttg ctcttttatt ttctagtatt
     1801 tctgaaatat actgcaaggc aattttttca gattctagat aatgaggact ttctgaaaag
     1861 aattctaaat ctttaaataa atctttacct attttctttt tcactgccat tgctattgct
     1921 atatcattac agaaagcaca ctcatttttg ggagagacaa atgcctttat taaactaact
     1981 agtttagaac tgaggctgag acttttttct gctttctcta aactctgggc aactgctatt
     2041 aatttcggag cacgagaata aataattttg ataggagcaa ttactttccc aaagctaatc
     2101 tttgaatacc aatacagtat tttcagaaat attcctttag gtgtatcaat tggttttaga
     2161 attagggatt ccatagaacc ctcctttttt gaatttctaa atcagggtcg atttcttcct
     2221 ttgatttgtg acaaaaaata gatttttttt caaagaatac tagttttcga ttctaaaatt
     2281 gcggtaaaac aggattattc cctagtattg agagaagtcc tttctcttga atgtggtgat
     2341 gattggataa aattattcta ccatacattt tatctaaatt aaatttcact tgtaattaga
     2401 tgtattgaaa ataggtttca taattagatt tccatgaaaa aattttattc aaagtatttc
     2461 ttaatcttct tttctttttc gattctctac tcaggttgca actattcgaa agagaatatt
     2521 tgtgatccaa attctcccat agcaaaatta tatttggtat ggagtgcttc taacaatttt
     2581 cttattcctt attgtgggat aaggcttgtt actcttcccg caccagttct ttccaactta
     2641 aaaaataatt ccattgtaga aacaggcttc ttgatcggaa cagctcctgc aggaacaagc
     2701 tcagtagaga ttagtctaga caatggatcc tttcaaacgg caactggcac tacgaattgg
     2761 acttacaaac ttccaacagg aacatctact tggagatatg gaactaaaca caggataact
     2821 gttagatcga acaccttagt cagtggtatg aatatggtat ctgaaacaag tagtattact
     2881 gttcgaaaag ggcacaacaa agatatcaac ggagacgggt atgctgattt agttgtttca
     2941 gcatttcaat atccatccgg cggtttacag ggtagagttt atatttttta ttcgagagga
     3001 agtagtggaa tcataaccac tacaacacaa actgggacag taagtaatgc agttatactc
     3061 actggcgagc taagtagcgc ctttggcaga agcgtaataa aagctgatat caatagtgat
     3121 ggctatgctg atttagtcat tggtgcaaca aatttaaata cgtctacagg tagagtgtat
     3181 attttcttat ctccaggcag tgctggaatt gccgctacct ctactcctca aacgagtgca
     3241 aattcagtga ttactggtga ggttacaaat agtaattttg gatctggagt aaccgttgga
     3301 gattttaatt tagatgggta tcctgatctt gtagtcaatg ctttctcttt ttcgactgct
     3361 acaggaagat tgtatattta ttattcttct gctaatggta tctcttcgcg cacggcaagt
     3421 gttgcaacgg atactactat tacaggagag acgacaaata acgtttttgg atatgtattt
     3481 gaaagcacag gggatattaa cggagatgga tatcctgact tagtcacagc ggcttatgtc
     3541 tataattcga aacaggggag agcttatatt ttttattctc agggaagtgc tggaattacg
     3601 ggaactctat cagcaagttc tccacccgca gggactacag tcatttcagg agaacctgga
     3661 attgagggta attttggagt cagtattaca gttggagatt ataatggaga tgggttttca
     3721 gatgtggtga ttggagctaa ctatgtggat acatctaggc aggggagagt ttatgtattc
     3781 cactctgctg gcaaatcagg agtaaccact acagcagcag cctctgctac aactatacta
     3841 actggagaga ctggaacaac taattctttt ggagcttatt gtgtttcctt agatggaaat
     3901 ttggatggtt atgatgattt agtttgtgga gctagtgctg cttccagtgg tgctttgaat
     3961 aaaatctatt attttcaatc ttcttcaaat ggaattgcaa ctggttttgc gaatcttgta
     4021 agtaatatca tcatcacagg agaaaatagc tctgatcaat ttggaagatt tttggctacg
     4081 ggtgatttta atggagatgg attttcagat attgctgtaa ctgctcttac tttgaattct
     4141 tctataggaa aatcttatat ttttcaaaat aatagaacga atgggtttac ttctgtaagt
     4201 gcaagtggcg caacttccag tcaagtagga gaaaatactt ccgatcgcta tggagaattt
     4261 gttcaataaa gaaaggtgaa tatgaaatat attaaatatc ttcttctggt tatactgcta
     4321 tctactctaa cgttattcgc cgatgaagtg actcttagaa atgaaaaggt cattgaaaaa
     4381 gtaaaagtta aaatttctgt tgataaagta gctgttacat atctagataa taaaacagaa
     4441 acctatccca aaaaagaagt taaatctatt cgtattaaac caattattat aaataatcct
     4501 gtcacggaaa aagacaaggc cgaaaacgaa aaagaaaaaa ttcgtgcagc ggaatcttta
     4561 caaaatctca ctggacttga aattcaagaa aaccaaaagt tgaaagtggc agttctaaat
     4621 tttaaaacaa gtgcaaatat tagcaaagaa gaagcggata taatcgtgga aacgattaca
     4681 gtcgagcttg taaaaacaaa gctttttatt ttagtcgatc caattttagt gaaacaagtt
     4741 atggatgaaa agggtggagt cggttgttcg gataatcctg ctaattgtaa aatgcctgca
     4801 gctgagattg caaaatcatt tcaagtttca aaaattgtaa caggcacaat caataagata
     4861 aaaaatagtt attacgttag tggaaatgtt attgatacga attctaaaaa cattgatttt
     4921 gctgaaagtt ctattgcaac ttcatttgaa aagattcatg atacatctga aaatttttct
     4981 aaaaaagtag ctggtggtat tttgagtatt tccaaaacag aaattaattt aaatcaagaa
     5041 agtaagggat tcttttctaa cttattcaaa tccaattcag aatccaaagc agataatatt
     5101 tctcaagaca gtccttctaa aatgcgttat gtgtggagat cggcacttct tcctggttgg
     5161 ggacaaattg cgaatgatag aaaattgaaa gggttttctc ttttaggatt atttatagtt
     5221 ggagttggat ttgaaattag ttcttataaa aattacaagt ctgcgatcga agattataga
     5281 agtgcaggag ataattttat cttaaacaca tctcttacaa atgcgaatgt agccggattt
     5341 gcattctatc ttaattctag aaatatcaat tcccaaagag atcaaattga tttgtatgaa
     5401 catagactgg aatccggcgc tggatttctt gcattgcttt atctttataa tctttttgat
     5461 gcctattatt ttgcgaaaga tcctgccaag tcagctttag attctagtag agagattact
     5521 cctaaattcg ctgcaattcc aacccgaaat tcagtggggc aaacttatac acaatataac
     5581 ttttctatcg aaatgagatt ttgaattgca aagagataag aaagattact ttttttctag
     5641 cggctatcgt ttaatttgca ttcaactttt tctagaatcg tctcccattt ctatcttaca
     5701 agtgaatggt ttaaatttat atcttcattg caactatata aatatagaca atagttttga
     5761 agcaatacat gtctgataat gaaggattct ttgaagcata ctaacaacta aatcttatcc
     5821 cattctggtg aagtctttct tgctttgact tttttcatta ctttatcaaa attcttttct
     5881 aacgactctt tggttttact tgctattctt tttttaaaga actgtgcagt ctctacatca
     5941 ttgagtcctc ttgtaaacgc atacaatgca aattgattga gagaaacacc ttgagcagat
     6001 gcagttcgtt ctaatctatc tttcaaatct tctggaattc gaacggttaa aacatttttt
     6061 ttagacataa aatttcctcc acattctggc aaagtcggtt ggtgtgatta ctttaaatga
     6121 atcaaataat aaattatttc caatcgtata atctcgagta ttcgaagtaa ttaaaaaatt
     6181 actattactt gcaaatgcta attctatgaa catattatct gcttcatctc ctaaattagg
     6241 acgcatacta aagctaatag aaaaaggaga tgctacacta caaatataat caaggaagtc
     6301 tttaatgtct tttttgctta gctctaaatc ttttaaagat ttttcacgta acaacacatc
     6361 agagtattcc atgaaaacag gaatagaaag acacaattca agcttcctct cacgcacaag
     6421 cctaacaata caattagatg ccccaccact atcacgcaat gcttgataga ttacattcgt
     6481 atctaaagta attctcacaa aaatatgata gcatatatac tatcataggt caagattttt
     6541 tctttctagc attatcgctc atttgcatct aacttctccc atttctatct tacaagtgaa
     6601 tggtttaaag caaaaaagga ttgcgagtct tttagaaaaa gaggttctga aatagatagg
     6661 ggaaaatttt ctgcataata tgagataaaa ataagtttta agtatactta aaactattta
     6721 caaaatattg aatagtttta agtatactta aaactatatc caaaggctaa aatcaatgaa
     6781 ttttgaaata tcagactttt cctttctaaa gagagacatc tatgtggatc ggtttcttcc
     6841 ttttttggat aagaatatca ttaaaattct tactggtcat agacgagtgg gtaagagtta
     6901 tatgctgttt cagattattg aaatgctaaa agaaaaaggt gttaagcctg aacagattat
     6961 ttatataaat aaagaattgg ctgaatttgc atccattaaa aatcacaaag atcttttatc
     7021 ttatgtcgaa aaatttagaa aaaagaaaaa acaaaaaata tatctattta tcgatgaggt
     7081 gcaagagata gaaaaattcg aaactgcact ccgtagtttg cttgcagaaa atatttatga
     7141 tatttattgc acaggaagca atgcaaatat tttatctggt gagttagcaa ctaatctagc
     7201 cggtcgttat attgagttta aaatttatag tctttcgtat ccagagtttc taaatttcca
     7261 taagttaaaa aataataaag atagtttaat gcagtatttt aaatttggtg gattgccttt
     7321 tttaattcat cttgatctca aagaagagat tgtatcagaa tacatgcgta atatttacaa
     7381 tactatttta ttgaaggatg tagttgcacg atatcaaatt agaaatgttg cttttttgga
     7441 aaggcttttg gaatatctcg cggataacac cggtagcctc gtatcggcta agagtattag
     7501 tgatttctta aaatcccaaa gaattaatat ttcccctaat gttattttaa attatatttc
     7561 ctatctggaa agtgcattta ttatttttaa agtgcagcga agagaggtgg gaggtagaaa
     7621 aatatttgaa attggagaaa agtattattt tgaagaaata ggaattcgca gaacattagt
     7681 aaactctaat atttcagata tacataaagt attggaaaat attatttatc tgcatctttg
     7741 cattgctggc tatgaagttc gcattggtaa actggacaac aaagaaattg attttgtctg
     7801 ttctagaaaa gaggaaaagc tttatattca agttgcctat ttaattgcgg atgaaaaaac
     7861 atttgaacga gagtttggga atctccttct aatcaaagac aactttccca agtatgtggt
     7921 ttcaatggat gaaagtatag aagggaatta taaaggcatt aagcatattc acattcaaaa
     7981 tttcttaact aaagttttgc aggataaact accttaactt ctcattgttt ctgtatatat
     8041 tggtcgcgta tcgtattatt tttaaaaagc gattctggaa taagattaat tttacactct
     8101 caatttttgc gaacttcaat agtagagttt atagattaca ttctattcag aacaattatt
     8161 tatagtgtat catacattta tattctattc cttttttctt gtatttctag cggctatcgc
     8221 taatttgtat cgaacttctt ccagaatcgt ctcccatttc tatattacaa gtgaatggct
     8281 taaagcgaaa aaagtagaaa ttatatctaa gataactcgg ttgattgact attctgtatt
     8341 attagaattg gaaaaaattc tagcaatctc tgaacagcaa acaaatgaat gggacagttt
     8401 aacagcctta gacaaagcta aaatcgaaaa agggttagat agcatacaga aagggaaagt
     8461 tatttctcat tcaatggtta aagaaaaaat agaccaattt attaaagagt tataatgaac
     8521 tatgatgtgg aatggtcgga agatgctctt tccgattatc gagagattct aaaatacata
     8581 ttatctgatt attggataac agaaaaaatc cagaattgat tcaagaagct ttagataaat
     8641 attgaaaatt caattcattg aattattcct ccacaagagc acaatgactc tatctagctt
     8701 ttattttatc cttgctcttt ggtagaaagg ttgtagaatt ggaagtataa gaggtaatag
     8761 tatgagttta tactttataa ttatggctac tattggttta atctgtgggg tttatttgat
     8821 tttttcaggt atggctggta ttatttcaaa gcgggttgct gatgatgtta caaatagatt
     8881 aaaaagtgaa atgaaacatg gagaataatg agtttactaa taaacttata ctttttcttg
     8941 gcatttgtat cgttggctta ataattctcc tcatgcaaac atactcggat ataaaagtgt
     9001 atgctgatac tattattacc tttatatctg taattgtttc cttaattgca attgttgatt
     9061 ttataaaaaa gcattccaga aaagattaac ttgtctttct agcggctatc gattaatttg
     9121 catttaactt tttccataat cgtctctcat ttctatctta caaatgaaag gcttaaaata
     9181 aaaaaggatt gcgagtcttt cataataaga tgttttgaac tcaacggggt aaaatttttg
     9241 ttctgtaaaa taaaataata tgtgaatgca aagaattcaa ttctttattt tgggctatta
     9301 gttattctta aaaaatgtat ctaatttgtg catttaattt caattttata attttcttca
     9361 taaaatgact cattcgttct gaaatcgatg gatatgccag ttacgacagg aaatactctt
     9421 tccttttttg aaaaaaagtc atagagatca ccgctcgatt ctaaaaacgg ataaagtagt
     9481 attcctgatt ttcccaaatc gggaatatat cgaaatgttc tcgaagaatc atccaaataa
     9541 tatgtaatga atattggctc ttctttatat aggatttttt ttaaaatccc atataaactt
     9601 tttcgaatat ctaacgatcc tacacaaata ttatttccaa ttgatgattt ctgtataatt
     9661 tcattctcaa aaattattgt agcatttggt ttacttattt gttttaattt tgtttcccaa
     9721 ttagccgatc tacttttatt accccttttc ttaagcagaa gaaagttatc tttaagaaga
     9781 atcgattcat ataagtcacg tatacgcaag aaagtttgca gctcatcatt taataaatac
     9841 cgatggtcta gggaataagg ataatgaaaa attatatatt ctggtgattt ttcatggtca
     9901 ttgaaaaaat taacattctg ttgaattaac cattcactag aagtaatgta agattggtaa
     9961 acgggtcgat tcttccaata aagacctgat gctaatactc tacctacttg atacggaaaa
    10021 atatcgacag attcgtttcc tattatatta atcatttcat ttggaagtaa tttcggtatt
    10081 gtaatttttt tttgatcgga ctcagaaatt agtctcagta atttttcaaa tttgtttata
    10141 tcaatgttga aatgagccaa acgatagtta gtcactacat cccaataaat cccgcccgga
    10201 gttgaaagaa cgtaaatgta aagaaacgca agagatatga gatatgagat atgagatatg
    10261 agat
//
LOCUS       DAOQOS010000164        10245 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_3440_length_10245_cov_5.956330, whole genome shotgun sequence.
ACCESSION   DAOQOS010000164 DAOQOS010000000
VERSION     DAOQOS010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 10245)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10245)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10245
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3440_length_10245_cov_5.956330"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..430)
                     /locus_tag="PLF66_23170"
     CDS             complement(<1..430)
                     /locus_tag="PLF66_23170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002981265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase zinc-binding domain-containing
                     protein"
                     /protein_id="HMZ67008.1"
                     /translation="MYVCDTDKTKKPNYIPTLERSSQYKAVWKRHWKEFVNQYDRRFK
                     AIYGKLTGEKIEEVEKLLGCGDFLNGFQRYSCEECDINLIVPFSCKSRLCLSCYRKKL
                     FGWSINLSHILNIDLRHIHVTFTIPVVRYARSDSATHQPDM"
     gene            959..1174
                     /locus_tag="PLF66_23175"
     CDS             959..1174
                     /locus_tag="PLF66_23175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010573985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67009.1"
                     /translation="MIIRSLQNPVYYRRKKGGLSGAELKPKPVQDRYNKTFEDYLKEA
                     FQGEVVQNGNWFSNQISDLTKRNLQRI"
     gene            1261..1863
                     /locus_tag="PLF66_23180"
     CDS             1261..1863
                     /locus_tag="PLF66_23180"
                     /inference="COORDINATES: protein motif:HMM:NF015935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HMZ67010.1"
                     /translation="MIAVLLGSGGKGRNVEILSKELLIEFTGILGLVTAPISSLKKQK
                     GLGKAKIATLVALKEIATRIKYKKLLTQDRFDLPSLLELLHLKALKEVRECFYLVTLS
                     EEKTLINIDLLARGSLTEVGVYTRDIVKQILDDASKYVIIAHNHPNQSSQPSREDYLL
                     FHRLRNLLSELEISLLDHLVIGIDGVHSCEENRKLVTIPS"
     gene            1932..2696
                     /locus_tag="PLF66_23185"
     CDS             1932..2696
                     /locus_tag="PLF66_23185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020771324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMZ67011.1"
                     /translation="MKFLQNLQVLWILVKRDYALQFAGSLLGVIWMLIQSLSLIAIYT
                     FVFFILNKTPNLPKSQINFSSYIFVGLLFWIPTQEMMIRGVSILTDNRQLIKRSTLKI
                     NFFLWIPFVQMMLHSFVISIPIFLILAIQGSIDYRLFLLGYLVLFFTGLYLMLVLEYL
                     ARLNILLKDISPIIRLFAQIIFWTLPILYFPDGVLKEVNNWNPFVVFLDLFRFLILKE
                     YALQFPVYFLFAPLFIFLFIFILTHLKFDEVVMDHL"
     gene            2693..3886
                     /locus_tag="PLF66_23190"
     CDS             2693..3886
                     /locus_tag="PLF66_23190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020774455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMZ67012.1"
                     /translation="MIRIRNLTKKYIGFASPLERILTGISFGFIHGSRSFTALNDISI
                     EVNRGEILGIIGRNGAGKSTLIKVLSGVSKFQSGTLEKTGSLRSILELGVGFNPELSG
                     IENVYYNGLVWGYSVNEIENLTDSIFDFSGLNEFRNVPLKNYSTGMMMRLGFALATAN
                     RPDILLVDEALAVGDASFQQKSLSRFQKFKEEGSAIVIVSHDLNLLQMISDRMLVMER
                     GKIAFIGESNLALKKYIQILAENSFESNETKTNLTSDVIESYSIQLSSNGIKQSIYAI
                     GEIVELSFSIKTKSFIPDLTIGFHIDDAKGLRIFGTNSYHKKSNLKNISKNSTIEYSF
                     QFPLNIAHGKYTLGFALHEGDNHTTNCYLWKDDVIEFEVERLSMPKFDGPAFLPVECK
                     WNIKS"
     gene            4516..6063
                     /locus_tag="PLF66_23195"
     CDS             4516..6063
                     /locus_tag="PLF66_23195"
                     /inference="COORDINATES: protein motif:HMM:NF019237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HMZ67013.1"
                     /translation="MSRVILFLRFICLIFLSTILNCTTSSYPNNCEFGSKAFLNNFIL
                     STVVLRMNSYCGITNSATAAANAGAGITFTSFSFTSAANDLNKTYTGTVTGNNIAVEL
                     PYGKITTAIPTIGTNATTILAGSTSILNGVTALDFTNPITFTLINSNGGKVVYTVTVY
                     AITPVEDTGQTACSDNNVIQPCSSMNATFPRQDAHFIDFPNPKGVQTPATNPGYPSDF
                     ITKNTLTGIVWKTCLEGKTGFDCSGGALSALTYANAVTACANLNSANSGAGYAGIKNW
                     RLPALQELMQGQVFLTYQINWDVTYFPTPVAAEKFPALRSSNLILPAATSSMIANGAG
                     GNDGMPSVLANSSTTSVRCVAGSPFPNGDLVDNNDGTILDKRSKLYWQKCALGQNNDA
                     TCSSGAIGRSWQNVLQDCNNLTILGKQWRTPNIIEFTSLIDTSQTSAPYINTSKFVNF
                     PTTAATSPTANNAKHFTIANSNLNNLAYQARINFATPTATPNGLDTKGNTIIGTNDYI
                     GRCVAGP"
     gene            complement(6319..6837)
                     /locus_tag="PLF66_23200"
     CDS             complement(6319..6837)
                     /locus_tag="PLF66_23200"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase activity [Evidence
                     IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HMZ67014.1"
                     /translation="MKILGITGSLRKNSSNTAILLAAQKLAIVGMEIEITDFIGRLPH
                     FNPDLDNESVDPSVLEWRGKLSLADGFLISCPEYAHGVPGSFKNALDWVVSSGEFMNK
                     PVLFLNASPIVKASLTETLTVMTANVLLDASIQIPFLRNKTDKEGNLVLEEEVIKQIQ
                     GCLEALRLHTNI"
     gene            complement(6840..7316)
                     /locus_tag="PLF66_23205"
     CDS             complement(6840..7316)
                     /locus_tag="PLF66_23205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67015.1"
                     /translation="MKRVLYLLAACIAIPVLARIGEMKVEKKTANPKNTKEDIVCCEI
                     NFLNGANDIKINKVCLGKGKTIIEMEFTKNYSACTILASNILVDNSGKQYSPTYHSGL
                     ANCPELSMARRGHKFYWGFEPIDKKAKSLRMKEDETMHPGMIAMEWTEISIEHCKW"
     gene            complement(7325..7810)
                     /locus_tag="PLF66_23210"
     CDS             complement(7325..7810)
                     /locus_tag="PLF66_23210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67016.1"
                     /translation="MKQILILLILAMLLFTSIVLARVESKVSGKSNVAPQKKNCCSIQ
                     KSFKNKISLTEICIEKNQTKIEFQILIPYTMCGNTNDIGLSDENGKSYEIVSMKNIPT
                     CPEIIQINPGYKFYWFFEKLDKGISTLDLTENKNQNLNPVFPNWEFWHWKNIDVSKCN
                     F"
     gene            complement(7820..8377)
                     /locus_tag="PLF66_23215"
     CDS             complement(7820..8377)
                     /locus_tag="PLF66_23215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67017.1"
                     /translation="MSLIVNFNSMHKPSNQKNTYLDKRITLAILLFLCFLFTPFQSIY
                     TSLKPSEKKITPKATAKKSPCCKLETYPEPNVQLVKICNTPNSTVIYMSLKENSDACS
                     YVDKITLEDNRGRVYKAIGMNGIANCPDINFIPKNTLFSWEFEKLDKGLTSINLEEDP
                     YAPKPRPDWNWWKWDNISISHCNLK"
     gene            complement(8355..8837)
                     /locus_tag="PLF66_23220"
     CDS             complement(8355..8837)
                     /locus_tag="PLF66_23220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67018.1"
                     /translation="MQKKIFFLFIAILMIQIPIYSNLRKGKDKFDPAPKKAKPDLKKE
                     NCCEIDSDFDNSVRLKRICLRDGMTSIQIKFLTPTFVCTILENLTLKDNAGNKYKPLS
                     ADGIPDCPRSESRKPGDSFLWNFEKINSGIKNITLSESEVASLSAWKWENVDVSHCKF
                     "
     gene            complement(8840..10006)
                     /locus_tag="PLF66_23225"
     CDS             complement(8840..10006)
                     /locus_tag="PLF66_23225"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002769446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HMZ67019.1"
                     /translation="MAIAELKGKEVPIKLWTKLDAVESEALTQLKNIASLPWAFHHVA
                     VMPDVHFGIGATVGSVIAMKDAISPAAVGVDIGCGMGAIRTNLKADQLPDNLTKIRSR
                     IEERIPVGHHEHKNPKIQSRTNALFAEFRSLHPKVMDLESKAVRQCGTLGGGNHFIEL
                     CLDTENTVWMMLHSGSRNIGKTLAEIHIKTAKKLSHNMYLPDRDLAVFLANTPEMEAY
                     RRDLFWAQRYALLNRQTMFVLYKKVLQEFFPNIEFEEPILCHHNYVSEETHFGEKVVV
                     TRKGAISAREGEWGIIPGSMGTKSFIVKGLGNPESFHSASHGAGRRVSRTQAKKEFSL
                     EDLAKQTSGVECRKDAGVLDEIPGAYKNIDEVIENQKDLVKVEYILKQILCVKG"
     gene            complement(10040..>10245)
                     /locus_tag="PLF66_23230"
     CDS             complement(10040..>10245)
                     /locus_tag="PLF66_23230"
                     /inference="COORDINATES: protein motif:HMM:NF025625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4357 domain-containing protein"
                     /protein_id="HMZ67020.1"
                     /translation="MKRLRDKLMNENIIKKTNEKFLLSKDYIFSSPSTAATIVMGRSA
                     NGKTEWKLENGKTLQEVEEFSEE"
BASE COUNT         3107 a         1856 c         1723 g         3559 t
ORIGIN      
        1 tcatgtccgg ttggtgagta gcagaatccg agcgagcgta tcgaaccacc ggaattgtga
       61 aggttacatg aatatgccgc aaatctatat ttagtatatg cgataaatta atcgaccaac
      121 caaaaagttt ctttcgatag caagacaaac aaagtcttga cttacaacta aatggaacga
      181 ttaagtttat atcacattct tcacaagaat atctttgaaa tccatttaga aaatctccac
      241 agcctaaaag tttttctacc tcttctattt tctcccctgt caactttccg tatatcgctt
      301 taaatcttct gtcgtattga tttacaaatt ctttccaatg tcttttccat acagccttgt
      361 attgactcga tctctctagg gtaggaatat aatttggctt ttttgttttg tctgtatcgc
      421 acacatacat tgtagaaaac ctctacaata gatgggtaaa aaattcgtat gtatgtgcgg
      481 gcttttggga aaaaattcca aatggaatcg tagactcagt agaataaata taaatctatc
      541 gtttccgtat cgtttagtta tcgttgtcat cggtggtaac ctttggaatc tcccttaaat
      601 tgacagcatt atccatcggt gtcggtgtcc tcggtgttcc tcgtcttcat cggtgctaat
      661 ctttggaatc tcccttaact tgagagcatt gcccatcggt ggtaatcttt gaaaaatttt
      721 ttgaatgaaa aattaatttt tttttgaaat tctctcattt tgtcctgcac aaatattatt
      781 cttattttat agcaagaaga gaggaaatct taaatttact gagaaaaatc aataaaatta
      841 agaaaaagtc ttgacaaatg ctaaacaaaa gttaagtatt taaaaataaa atttattcat
      901 ttttaggttg acataatagt tttttatgtc gtctaattaa atggaacgga ggcagacaat
      961 gattattagg tctttgcaaa atccagttta ctatcgtagg aaaaaaggtg gtctttccgg
     1021 agcagagttg aaaccaaaac ctgttcagga tcgatataac aaaaccttcg aagattatct
     1081 aaaggaagct tttcaaggag aagttgttca aaatggcaac tggttctcga atcagatctc
     1141 tgatttgact aaacgcaatc tgcaaagaat ctgagcctct catttgcaaa gggtcattct
     1201 gtaaagactg accctcgttc tagagctatt cgtgaccctc aaaagttaga agactgggaa
     1261 ttgattgcag ttctattggg aagtggtggc aagggacgaa atgtcgaaat cctctcaaaa
     1321 gaacttctca ttgaatttac cggaatttta ggacttgtta cagctccaat ttcttctcta
     1381 aaaaagcaaa aaggcctcgg gaaggcaaaa attgcaacac tcgtcgctct caaagaaatt
     1441 gcgacccgca ttaaatataa gaaattactg actcaagatc gattcgattt accttcactt
     1501 ttagaattgc ttcatttaaa agccttaaaa gaagtgagag agtgttttta cttagtaaca
     1561 ctttcagagg agaagacact cataaacata gatttacttg caagaggaag tttaaccgaa
     1621 gtaggagtct atacaaggga tattgtgaag caaattctag atgatgcgag taaatatgtc
     1681 atcattgctc acaatcatcc caatcaatct agtcaaccaa gtcgtgagga ttatttactt
     1741 tttcatcgct tacgaaatct tctttcagaa ttagaaatta gtctgttaga tcaccttgtt
     1801 attggaatag atggtgtcca ctcctgtgag gagaatagaa aattagttac aattccaagt
     1861 taaaaaaata atgcagctcc aaatcaatca ttgattcaac agaaactatt taaattatag
     1921 cattatattc tatgaaattt ttacagaacc ttcaagtctt gtggatactc gttaagagag
     1981 attatgcact acaatttgca ggtagtttgc ttggtgtgat ctggatgctg attcagagtc
     2041 ttagtttgat agcaatttat acttttgttt tttttatact aaataaaact ccaaatcttc
     2101 ccaagtctca aatcaatttt tctagttata tatttgtagg tttattattt tggattccca
     2161 cacaagaaat gatgattcgg ggagtaagca ttttaactga taatagacaa ttgattaaaa
     2221 gatcaaccct taagatcaat tttttcttgt ggataccttt tgtccaaatg atgcttcata
     2281 gttttgtaat ttcgattcct atttttttga tactcgcaat tcaaggatca atcgactatc
     2341 gcttatttct acttggatat ttagttcttt tttttacagg tctctatttg atgttagtct
     2401 tggaatattt agcaagattg aatattttac taaaggatat atctccaatt attcgcttat
     2461 ttgctcaaat tattttttgg actttaccca ttttatattt tccagatggt gttttaaagg
     2521 aagtaaacaa ctggaatccc tttgttgttt ttttggattt gtttcgcttt cttattttaa
     2581 aagagtatgc tttgcaattt ccagtatatt ttcttttcgc tcctctattc atttttctat
     2641 ttatctttat actcacacat ttaaaatttg atgaagtagt gatggatcat ctgtgattcg
     2701 aattcgaaat cttacaaaaa aatatattgg ttttgcatct cctttagaaa ggattctgac
     2761 agggattagt tttggcttca tccatggaag tagatctttt actgcgttaa acgatattag
     2821 tatagaggtg aaccgtggtg aaattctagg aatcatcgga cgaaatggag caggcaaatc
     2881 cacattgatt aaagttttat ctggggtttc taaatttcaa tcgggcacac ttgagaaaac
     2941 cggttccctt agatctattt tagaattggg tgtaggtttt aatccagagt tatctggaat
     3001 tgaaaatgtt tattataatg gtctcgtctg gggatattct gttaatgaga tagagaattt
     3061 aacagattca atatttgatt tttctggatt aaatgaattt agaaatgttc ctttaaaaaa
     3121 ttattcgacc gggatgatga tgcgacttgg ttttgcccta gccactgcaa accgaccaga
     3181 catactcctt gtagatgaag cgttagccgt aggagatgca agttttcagc aaaaatcact
     3241 ttctagattt caaaaattta aagaggaagg atctgccata gtaattgtaa gccatgattt
     3301 aaatctactc caaatgattt cggatagaat gcttgtgatg gagagaggta aaattgcatt
     3361 tatcggagag tctaatcttg ctctaaaaaa atacattcaa attctagcag aaaattcttt
     3421 tgaatcaaat gaaacaaaga caaatttaac atccgatgtg attgagtcct actcaattca
     3481 actatctagt aatggaatca aacaatctat ctatgcaata ggcgaaatag ttgagctttc
     3541 tttttccatc aagacaaaat cttttatccc agatttgact attggttttc atattgatga
     3601 tgcgaaggga ctcagaattt ttggaacgaa ttcctatcat aaaaaatcaa atttaaaaaa
     3661 catttcaaag aattctacca ttgaatattc ctttcaattt cccttaaata tagcacatgg
     3721 aaaatatact cttggttttg cactacacga aggagacaat catacaacaa attgttatct
     3781 gtggaaagat gatgtgattg aatttgaagt agaaagactc agcatgccta aatttgatgg
     3841 tccagctttt ctgcccgttg aatgtaagtg gaatattaag agttaaacac attatgttgc
     3901 gacccttagg aagcaacatt gagtttgaac acagataaag acggatggtt ttaatttctt
     3961 tatttttatc agtgtctatc ctgcccttcg atccaactat tcgcgaccaa cgccttcgca
     4021 tggggtgaaa tatttttaca tcgaactcag gtcatttatc ataattccaa aaagatataa
     4081 tgaatcaaat attctattct ataccaattc ccaaaagtaa tttcccataa ggactttatt
     4141 tgagtagcat ttggtatacc gaacatactg atgttcggga acactaagaa gtgtttagac
     4201 caaacccgcc ttcttatttt tgaaatggga tttttttttg acgtttggta taaacacaaa
     4261 gatctaggtt tttaggtaaa agcagtttgg acaattccgg tagaggattc gtaatttatt
     4321 taaatgaagt aaagaatccg tctcaattaa taaaatctgc tctgctttcc ttgcgtattt
     4381 gttacggtaa ataaacgtta gccgcatgtt atggctatgg aaggcttaag aaaatatttt
     4441 tgcttgcttt tttttaattc tctgaattcc tattttgctt cttctattag tttatagatt
     4501 tagaggaagc ttttcatgtc tcgtgtaata ttatttttaa gattcatctg cttgattttt
     4561 ttatctacaa ttttaaactg cacgacttct tcttatccaa ataattgcga attcggctcc
     4621 aaagcttttc taaataattt tattttatca acagttgttc ttagaatgaa ttcttattgt
     4681 ggaattacaa attctgcaac cgcagcagca aatgctggag cgggaatcac ttttactagt
     4741 tttagtttta cgagtgctgc aaacgatttg aataagacat acacaggaac tgtaacagga
     4801 aataacatag ctgtcgaatt gccctatgga aaaattacta ctgcgattcc aaccattggc
     4861 actaacgcta cgactatttt agctggttct actagtatat taaatggtgt aactgcttta
     4921 gattttacaa atccaattac attcacttta attaactcca atgggggcaa agtggtatat
     4981 accgtaacag tgtatgcgat aacacccgtt gaagataccg gacagacagc ttgttctgat
     5041 aataacgtaa ttcaaccttg ttcttcaatg aatgctacct ttcctagaca agatgcacat
     5101 tttattgatt tcccaaatcc aaaaggagtc cagactccag ctacaaatcc agggtatcct
     5161 tccgatttta taacaaagaa cacactgaca ggaattgttt ggaaaacttg tttagaagga
     5221 aaaactggtt ttgattgttc aggtggcgca ctcagtgccc taacttatgc aaatgcagtt
     5281 acggcatgtg ccaatttaaa ctcagcaaat tcaggagctg gctatgctgg gataaaaaac
     5341 tggagactgc ctgcacttca agaattaatg caagggcaag tttttcttac ctatcaaatc
     5401 aattgggatg ttacctactt ccctacgcct gttgcagcag aaaagtttcc agcattaaga
     5461 tcttcaaatt taattctacc tgctgcaaca agtagcatga ttgcaaatgg ggcgggcgga
     5521 aatgatggaa tgccatctgt attggctaat agctcaacaa cgagtgtccg ctgtgtagca
     5581 ggctcacctt ttccaaatgg agatttagtt gataataatg atgggactat tctagacaaa
     5641 cgttcaaaac tatattggca aaaatgcgct ctaggtcaaa acaatgatgc gacatgcagt
     5701 tcgggagcca ttggaagatc ttggcagaac gttttacaag actgtaacaa tcttaccatt
     5761 ctaggtaagc aatggagaac acctaacatt attgaattta cttctctaat cgatacatca
     5821 caaacttccg caccctatat taatacttct aaatttgtta attttcctac caccgccgct
     5881 acatcaccta ctgcgaacaa tgcgaaacac ttcactatag caaattcaaa tttgaataat
     5941 ttagcctatc aagctagaat taattttgca acacccaccg ctacaccaaa tggtttggat
     6001 acaaaaggga atactattat tggaaccaat gattatattg gacgctgtgt ggctggtccc
     6061 taattctatt ttcagaaaga tatttatttt tactcaatcg tatccaatac ttgctcatct
     6121 tctcttgata gattccgaaa tgccaaacca attccatttt catcaattcg aactacacct
     6181 gtatgtagac ttaattcttt cgtaggtgtt tttaaaagaa tatctaagcc agaatttaag
     6241 ggaaggtcta attcctttga ttcaatgtag agcccacttg tgctgatatc tttagtggct
     6301 aatactcatt taccctaatc agatgtttgt gtgtaatctt aacgcttcta aacatccttg
     6361 aatttgtttt atgacttcct cttctaaaac caaatttccc tctttatcag ttttatttcg
     6421 aagaaatgga atttgaatcg aagcatctaa taaaacgtta gctgtcataa cagttagagt
     6481 ttcagttaaa gaagctttta caattggaga cgcattgaga aataaaacag gcttattcat
     6541 aaactctcca ctagatacaa cccaatcgag tgcattcttg aaagaacccg gaactccatg
     6601 cgcatattct ggacaagaaa ttaaaaatcc atcggctaat gaaagttttc ctctccactc
     6661 aagaacagat gggtcaactg attcattgtc taaatccgga ttaaaatgag gaagccttcc
     6721 aataaagtct gtaatttcta tttccatacc aactatcgct aatttttgag cggcgagtaa
     6781 gatggcagtg ttcgatgaat tttttctgag actgcctgtt atacctaata ttttcatttt
     6841 taccatttac aatgttcaat gctgatttct gtccattcca ttgcgatcat tcctgggtgc
     6901 atggtttcat cttctttcat acgaagagac tttgcttttt tgtcaattgg ttcaaatccc
     6961 cagtagaatt tatgacctct tcgcgccatg gatagttctg gacaattggc tagtccactg
     7021 tgatatgtgg gtgaatattg tttgccgctg ttatctacta agatgtttga ggcaagtatt
     7081 gtgcaagcgc tatagttttt tgtaaattcc atttcaataa ttgtcttccc ttttccaaga
     7141 cagactttat tgattttgat atcattggct ccatttaaaa aattaatctc acagcacaca
     7201 atatcttctt ttgtattttt aggatttgct gtcttttttt ctactttcat ttccccaatt
     7261 ctagcaagca caggaattgc gatacaagcg gctaatagat ataatactct cttcattctc
     7321 tctcctaaaa attacatttg cttacatcaa tatttttcca atgccaaaat tcccaattgg
     7381 gaaatactgg attcaagttt tgatttttat tttctgttag atctaaagtt gaaataccct
     7441 tatccaattt ttcaaagaac caataaaatt tataaccagg attgatctgt ataatttcgg
     7501 gacaggttgg aatatttttc atgctgacta tttcatacga ttttccattt tcatcagaga
     7561 gacctatatc attcgtattt ccacacattg tatagggaat taaaatttga aattcaatct
     7621 tggtttgatt tttctcaata caaatttctg ttaaactgat tttattttta aaactttttt
     7681 gaatagaaca gcaatttttc ttttgtgggg ctacattaga tttgccgctt actttcgact
     7741 ctacacgagc aagcacaata gatgtaaaaa gaagcattgc tagaattaga agtattaaaa
     7801 tttgcttcat acgaaaatcc tattttaaat tacagtggct aatcgaaata ttatcccact
     7861 tccaccaatt ccaatctggt cttggttttg gagcataggg atcttcctct aagtttatgg
     7921 aagtcaatcc tttatctagc ttttcaaact cccaggagaa gagggtattt ttaggaataa
     7981 aattaatatc tggacagttg gctattccat tcatcccgat tgctttatag actcgaccgc
     8041 gattgtcttc taaggtaatt ttatctacat aagaacaggc atctgaattt tcttttaagc
     8101 tcatatagat cactgtagaa tttggagtat tgcatatttt gaccaattga acgttaggct
     8161 ctggatatgt ttctaattta cagcaaggac tttttttggc agtggctttg ggagtaattt
     8221 tcttttcaga gggttttaag gatgtataaa tagattgaaa tggagtaaac agaaagcaaa
     8281 gaaataagag gatagccagt gttatccttt tgtctagata agtatttttt tgattcgaag
     8341 gcttgtgcat agaattaaaa tttacaatga gagacatcta cattctccca tttccatgca
     8401 cttaaagaag ctacttctga ctcagaaaga gttatgtttt tgattccgga attgattttt
     8461 tcaaaattcc aaaggaagga atctccaggc tttctgcttt cacttctcgg acaatctgga
     8521 attccatcgg cacttagcgg tttatattta ttacccgcat tgtcttttag tgtaagattc
     8581 tctagaatcg tgcaaacaaa cgttggagtt agaaatttga tttgaataga ggtcattcca
     8641 tccctcaggc aaattctctt taaccgcaca gagttgtcaa aatcagaatc tatctcacag
     8701 cagttttctt tttttaaatc tggctttgcc tttttaggag cgggatcaaa tttatcctta
     8761 ccctttctta agttagagta tatcggtatc tgaatcatta aaattgctat gaataagaaa
     8821 aaaatctttt tttgcatatc taccccttaa cacagagaat ttgttttaat atatactcaa
     8881 cttttacaag atccttttga ttttcaatca cttcatctat atttttataa gcccccggta
     8941 tttcatctag aacccctgca tcttttctac attccactcc gcttgtttgc ttagctaaat
     9001 cttcgagtga aaattctttt tttgcctgtg tgcgactgac tcttctgcct gccccatgag
     9061 aagcggagtg aaaagattct ggatttccaa gtccttttac gataaacgat tttgttccca
     9121 tagatccagg tataattccc cattctcctt ctcgcgcaga gattgctcct tttcgcgtaa
     9181 cgactacttt ttctccaaaa tgagtttctt cggaaacata attgtgatga cataggattg
     9241 gctcttcaaa ttctatatta ggaaaaaatt cttgtaaaac ttttttgtat aggacaaaca
     9301 tagtttgtcg attgagtaga gcgtatcttt gtgcccagaa taaatctcta cgataagctt
     9361 ccatctcagg cgtgtttgcg agaaatacgg caaggtctct atctggcaaa tacatgttat
     9421 gcgatagttt ctttgcagtt ttaatatgaa tttctgctag agttttacca atatttctag
     9481 atccagagtg aagcatcatc cagactgtgt tttcggtatc taaacaaagt tctataaaat
     9541 gatttcctcc accaagcgtc ccacattgac gaacagcctt actttccaag tccatcacct
     9601 ttggatgtaa acttctaaac tcagcaaaga gtgcgttagt tctactttgt atctttgggt
     9661 ttttgtgctc atgatgaccg actggaattc tttcttcaat tctacttctg attttagtaa
     9721 gattatcagg aagttgatcc gcttttagat tggtgcgaat tgctcccatt ccacaaccaa
     9781 tatccactcc aactgctgct ggactgatag catccttcat tgcaatgacc gatcctacag
     9841 tagcgccaat tccaaaatga acgtcaggca ttacggcgac atgatgaaat gcccaaggca
     9901 gacttgcaat gtttttgagc tgtgtcaacg cttccgattc gacagcatct aactttgtcc
     9961 ataatttaat gggaacttct tttcctttta attctgctat tgccatcttg ttctcctttt
    10021 tatgtcgata tgcgaaatcc tattcttcgc taaattcttc tacttcctgt aatgtctttc
    10081 cattttctag cttccattca gtttttccgt ttgcacttct tcccattaca attgttgctg
    10141 ccgtagatgg actagaaaaa atataatctt tactcagtaa aaatttttca tttgttttct
    10201 taattatgtt ttcattcatt aatttatctc gtaatctttt cattg
//
LOCUS       DAOQOS010000165        10175 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_3471_length_10175_cov_5.815514, whole genome shotgun sequence.
ACCESSION   DAOQOS010000165 DAOQOS010000000
VERSION     DAOQOS010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 10175)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10175)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10175
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3471_length_10175_cov_5.815514"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..447)
                     /locus_tag="PLF66_23235"
     CDS             complement(<1..447)
                     /locus_tag="PLF66_23235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016549405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkene reductase"
                     /protein_id="HMZ67021.1"
                     /translation="MSSKLFSKTKLGNLELKNAFVMAPMTRCRAIGNIPNDLMATYYS
                     QRAGAGLIVTEGTSPSPNGLGYARIPGIFSKAQVEGWKKVTSAVHEKGTKIFVQLMHV
                     GRIGHVDNLPEGAELVAPSAIAAKGQMWTDKNGMQDHPVPREMTKSD"
     gene            complement(641..2227)
                     /locus_tag="PLF66_23240"
     CDS             complement(641..2227)
                     /locus_tag="PLF66_23240"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HMZ67022.1"
                     /translation="MKTKKSAFDFSTGGVGLFSFFYGYLYGFQINAVTVPPSITSASI
                     LPDVTVTFSSNLLASSATNNSVKLFDKNGTQITTTMSVSGNTLKVSANSMLSFSSIYT
                     LSLTTQLSDSSGRTLSSNYSTSFTTDTGTDHFLRSSSLNIDGTTPLGYIISSRVYPTT
                     DKLYFAWAEKADATLGNIAFLHVKSIDKNKSVSLIGTNLNYDGSKSADGPSMSSIDNT
                     LYLAWRENVEIRVKSFNGSSWTSSDSGSLTSVPTPIATDRPYLHTFNGKLYCIFGEEI
                     STGARLVRIKELNGTTWSFIDGGLLNSKNPTSTDGAGYKPVLITYNNILYAAWMEKNQ
                     STGGLDVRIKKYNGSNSWSFIDNGGIQYNNGMSTNADISPYLVTLVSNNNKLYSFWIE
                     DISGSSRQIRGKVFNGNDTSPSWTSIDNGSSLSYGGANSLTYALTSISHNNRVFILWQ
                     EFTSGVVQLRLATFSGEDSAPRWFFVDSNPTTDKGLTFNTTKSAAQGSMGSFNGNLYI
                     GWSEQFSGNEYNAYVSRMAF"
     gene            2709..3584
                     /locus_tag="PLF66_23245"
     CDS             2709..3584
                     /locus_tag="PLF66_23245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HMZ67023.1"
                     /translation="MNKKKSILLIVILLVLVYIPWFFSSMVLLPKTNCSKEHHVFCDT
                     PSELNLKYETIKIKNQDGLELESWFIPADNSDKAILLVHGHGGSRNEGLRFAKVLNES
                     GYNLLALSLRRNSGEFATMGFYEVNDVKAAVDFLIQEKKIKTVGIFGFSMGAATSILA
                     MEKDNRIKAGLFSSGYASALNVLSEAANRDFGIPYYPLIPIVREVLNFRAKMKIEDVR
                     PVDKIGNISPRPILIFHCDKDDYVDSSHAEELFKNAKEPKEKWVPACNKHEFIWNTHK
                     EEAELRASNFFKKNL"
     gene            complement(3608..4180)
                     /locus_tag="PLF66_23250"
     CDS             complement(3608..4180)
                     /locus_tag="PLF66_23250"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HMZ67024.1"
                     /translation="MGPRERILIVATKLFYEQGYVNTGINQIIQESKTAKASFYDYYP
                     SKDILGRAVLRKFCADVLFWLRQILRKSKTPDLFAKNLCSAIKKQIKEEKGFYQGCPI
                     ALFCAQLPSENDFFKQDFKEFVKIWETISNKYFLELRKNKKLNPTLKIPDLTRRVFNL
                     YEGALIMWKLSEDINYINQLETDLNKVFNS"
     gene            4288..4725
                     /locus_tag="PLF66_23255"
     CDS             4288..4725
                     /locus_tag="PLF66_23255"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015679322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HMZ67025.1"
                     /translation="MKTFHQTEIPVLWSHLDSNHHVNNAIFQFYFDEARMQALESTGF
                     NLAELRKINVGPVIYKSEIEYKKPLYHPETAIIQTWFEGLEKVRGITCQNLYRKSDGE
                     LVCKARFYSIFFDLEKDKPWKIPLELGENLRELSGPSKNTLAA"
     gene            complement(4837..6237)
                     /locus_tag="PLF66_23260"
     CDS             complement(4837..6237)
                     /locus_tag="PLF66_23260"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002183126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HMZ67026.1"
                     /translation="MLKERNQTFKLMFIGLDLVISFSGFWIAFLLRYFLDKSFYFNTS
                     NYIILSIVLSITQVLAFISIDLYHPRRGLAFFDELVVIASGVILNLLFILSLLFFFRG
                     EVRGESFSRLFVAYYAVTNILLTALVHYIFRIVLMRLRERGYNLRNVIIIGTGKTAER
                     ITDVIQGHKIYGYNIVGYIKSISKSDTRSDFKIIGNLKDIEKILLEYKPDLLIYAKEF
                     ADSENLREIIDICDHENIELKIIPEFIEFIAAKGRVEGMDGVPIISIRDIPMRMGYNR
                     FIKRSFDFVFALFFIILFSPVYLIISILIKLTSEGDIFIRQERVGLDNKSFFMLKFRT
                     MYTQAKTDSDTIWTTKNDPRVTPIGGILRKLSIDETPQFFNVLAGNMSVVGPRPERPF
                     FVEKFKNEHRQYMRRHAVKAGITGWAQVNGLRGDTSIEKRIEADIYYIENWSIFFDLK
                     IILLTPFTGLINKNAY"
     gene            complement(6237..9473)
                     /locus_tag="PLF66_23265"
     CDS             complement(6237..9473)
                     /locus_tag="PLF66_23265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS-assembly protein LptD"
                     /protein_id="HMZ67027.1"
                     /translation="MQNKKSVRLISIYRRVRVFRSYFVLLLFSLSISIFSQDTTELIR
                     QINVDGNEPKKTEADTIKEFRISETQKKLVRKVLDILSDREVDEYLRAMGLSIEGSIY
                     AKRARLREAVVPKDEKVKEEFNLQKEANKKQDSFIIENASEGELLSVDKTKSGVLILR
                     GKVKLKIGQGNLIADSIFVDSKKNEIYAEGNVEFVDNSLTVKGEKFIYDVKMERGVVY
                     DTKATLYPSFFVGKKIKKIDENKYVLDMGHFTACGAEVPHYKFKAKRIVFYSDNSIIA
                     TNLKFQVGGTELFWIPMYYSSNLGSGWVTQFGKNRSQGSFFQGHYQWSDPTAVPSLFM
                     PVGRRVKVDFYQVTGQAIAFDFWKTSPWLNYNLETGLANFKSYRTVDTYESRYGVPKD
                     PIGDVSTLMATNQVDKGDLCAYDNDKLKLPGGRTDCLATASQVLQANNPNWSYPIRNI
                     GLQQQDWSKINLRANARNNNIAGDGTRNLQIQFERYTNPRYDYEFGFRYEPANTLQSL
                     YTRRQQRNPFIRQNTTWALDYSQTHGDLSVNISARRNYFYLPYNPAVYSDFYPLYEEL
                     PRTIIRNSSQVGMLPYFYSPVYWDINVSNVVKRFYGSPARNSQSISSGVIPGLSGSYP
                     FGDPKASVARTEIETVAETGLKTNLNFGGYLTFSPSAYYGFQKYSLDVPGGIQTTDLS
                     RDRELRRNSYIYTRQSHKLSFGVPALLLSSTYRWTSGSRSELPEQTLMRGRDRVRELE
                     FALESNAFENVEFSIKTIRDLREFSREYNPQPTNQQRWYFTVARAAVFFDFIDGFGKK
                     RETLLEKRRSFYTGIFFNNDYVYHTAQNSPLYNNLTVSYKMGGFSLPFIRNFKNFEVG
                     GTWFHVFRNSQLEQFGVYAGTYNQAGKYYNTRSSFLDSYRFYIQTDVQVTRNLGIELE
                     LDSRVTQPWRFTDEIGQNAIFRNGTDQATIDNTYQQYTSPNTAFQQTTIQRDIANGLG
                     LNGSQARQTTAFNVNRFILIGKYNIHNFEYRVGYAMDLRAIPGGTSLDSLVSFYDQSV
                     FFSINLINVNLGPEEPAAAQTRARIYRFRKRPLDPGIGGYSGISAEGP"
     gene            complement(9499..9936)
                     /locus_tag="PLF66_23270"
     CDS             complement(9499..9936)
                     /locus_tag="PLF66_23270"
                     /inference="COORDINATES: protein motif:HMM:NF021435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2141 domain-containing protein"
                     /protein_id="HMZ67028.1"
                     /translation="MFFKRIMLLLIFIYSFALAGEDLCSLKVEIIKFRNNDGQALINL
                     FNKSDGFPSKRDKAFQSQKLPIHSNMATFHFKDIPCGEYAIGIMHDENNNLKMDTNLI
                     GIPKEGYGVSNNVKSKLGPPKYEDAKITLQSANTIIQIEMNYF"
     gene            complement(9990..>10175)
                     /locus_tag="PLF66_23275"
     CDS             complement(9990..>10175)
                     /locus_tag="PLF66_23275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002978190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HMZ67029.1"
                     /translation="IRANIKAHTVVVAGHVTGNVIATERLEMQSTGKVVGNIKTAKLQ
                     IADGVVFDGNCEMIQKT"
BASE COUNT         3324 a         1859 c         1590 g         3402 t
ORIGIN      
        1 gtctgatttt gtcatttctc ttggaactgg gtggtcttgc atcccgtttt tatcggtcca
       61 catttgaccc ttcgcagcaa ttgctgaagg tgcaactaat tcagctcctt ccggtaaatt
      121 atcaacatgt ccaattcttc ctacatgcat tagttggaca aaaatttttg ttcctttttc
      181 atgaacagca gaggtcactt tcttccaacc ctccacttgc gctttcgaaa aaatacctgg
      241 aattcgggca tagcctaatc cattcgggga gggtgatgtt ccttccgtaa ctatgagacc
      301 tgcaccagct cgttgtgaat agtaagttgc cattaaatca tttgggatgt ttccgatagc
      361 tctacaacga gtcataggtg ccattacaaa tgcatttttt aattcgagat ttcctagttt
      421 agtttttgaa aataattttg aactcatttt cgatcctttt aagtgtaaca atttacttta
      481 cactacaaaa tattctggaa ttaaattcaa gtataataat tcgatattta actatttttt
      541 tatttatttc aacatttgcc ttatatttca agggttatta ccctaaataa ctaaaaatat
      601 tcttattgaa atagaaataa ttttagtttt tcgaataaaa ctaaaatgcc attcgactta
      661 cataggcatt gtattcattt cctgaaaatt gttcagacca acctatgtat aaatttccat
      721 taaaactacc catcgatccc tgtgcagcac tctttgttgt attaaaagtt aatcccttat
      781 cagtagtagg atttgaatcc acaaaaaacc atctaggtgc agagtcctct cccgaaaaag
      841 ttgcaagccg taactgaaca actccagagg taaattcttg ccagagaata aaaacccgat
      901 tgttatggga aatggaagta agagcatacg ttagactatt cgctccacca tagctcaaag
      961 aagagccatt gtcaatcgat gtccaactag gcgatgtatc gttcccatta aaaactttac
     1021 ctctgatttg ccttgaactc cccgatatat cctctatcca aaaagaatat aacttattat
     1081 tatttgatac caaagtaact agatatggac taatatctgc gttagtagac attccattat
     1141 tgtattgaat tcctccattg tctataaaac tccaagaatt acttccattg tatttcttaa
     1201 ttcgcacatc taatccaccc gtagattgat ttttctccat ccaggctgca tatagtatat
     1261 tattgtaagt tattaagact ggtttatatc cagctccatc ggtagaggtt ggatttttcg
     1321 agtttaataa tccgccatcg ataaaactcc aagtcgttcc attgagttct ttgattctaa
     1381 ctaaacgggc acctgtagat atttcctctc caaatataca atataacttt ccattgaatg
     1441 tgtgtaaata gggtcgatct gttgcaatag gagtaggaac acttgtaaga gatccactgt
     1501 cggatgacgt ccaagaggaa ccattaaaag attttaccct tatttccaca ttttctctcc
     1561 atgcaagata gagagtatta tctatactac tcatgctagg tccatcggca gatttagagc
     1621 catcatagtt tagattcgtg ccaatcaagg aaacagattt atttttatca atggatttta
     1681 catgtaaaaa tgcaatattt ccaagagtag catctgcctt ctcagcccaa gcaaaatata
     1741 atttatcagt ggtaggatat actcgcgagg atataatata cccaagagga gtagtcccat
     1801 caatgtttaa actagaactt cgtaagaagt gatctgttcc tgtatccgtt gtaaacgaag
     1861 tggaataatt agaacttaaa gttcgaccag aactgtcact gagttgagtc gttagactca
     1921 atgtataaat tgaactaaaa ctaagcatcg aatttgcgga aactttcaaa gtgtttccag
     1981 aaacactcat tgttgtagtg atttgtgtcc catttttatc aaagagttta acagaattat
     2041 ttgtagcact agaagccaag agattagagg aaaaagtaac agttacatcg ggtagaatac
     2101 ttgcagaagt tatggagggt ggaacggtca ctgcattgat ttgaaaccca tataaatagc
     2161 cataaaaaaa actaaataag cctactcccc cggtggaaaa atcaaatgca ctctttttcg
     2221 ttttcaaaca gaatgttcct gttagaaaaa taaggatgaa aatgaaagtt atttttttcc
     2281 tcattagatt actcccaaat ctactaaaaa atttaatcgt gtgagaaaac cattctattg
     2341 gcactctcta taactatgtt tgttgttatt gatattactg taaaatattt atttactatc
     2401 tgtaattgat tcaaaactaa aaacaggata ttgaaagatt agaaagtaag aattattaaa
     2461 agaaaacttt agaattactt tgcaattacc ccatcaaaat attttatttg gattttttgt
     2521 aaaaccttgt atcctatttt ttttaagcta tttattggtt ccgatttcaa tttttaaaaa
     2581 gcaatgactg aaaaaaatca ggattgataa cacaatgttt agaaagatac aataaatctt
     2641 ggataggaaa ttatacgaat cttaaaaatt gcttgtagaa agtttaaatt aaatttgagt
     2701 tttcatttat gaataaaaag aaatctattc tcctcattgt tattttatta gtattagttt
     2761 atatcccttg gttcttctct tctatggtat tactacccaa aacaaattgt tccaaagaac
     2821 atcatgtctt ttgtgatact ccttctgaac tgaacttaaa atatgaaacg attaaaatta
     2881 agaatcaaga tggattagaa ctagaaagtt ggtttatacc tgcagataat tctgataagg
     2941 caattttact tgttcatgga cacggtggtt ctagaaacga aggtcttcga tttgctaaag
     3001 tcttaaacga atcaggatac aatctcctag cactgagcct aagacgtaac agtggtgaat
     3061 ttgcaactat gggattttat gaagtaaacg atgtgaaagc tgccgttgat tttttaatcc
     3121 aagaaaagaa aatcaaaacg gttggaatat ttggattttc aatgggtgct gcaactagta
     3181 ttctagctat ggagaaggac aatcgaatca aagctggtct ttttagcagt ggttatgcct
     3241 cagcgcttaa cgtattatcc gaggcggcta accgtgattt tggaattccc tactacccac
     3301 ttattccaat tgtgagagaa gttttaaact ttagagctaa aatgaaaatt gaggacgtta
     3361 gacctgttga taaaattgga aatatctccc caagacctat tctcatcttc cattgtgata
     3421 aggatgatta tgtagattct tcccatgcag aagaattatt taaaaatgca aaagaaccaa
     3481 aagaaaaatg ggtccccgct tgcaataaac atgagttcat ctggaatact cataaggaag
     3541 aagctgaatt acgcgcttct aattttttca aaaagaatct ttaatacccc agctagggca
     3601 agatgagcta agaattaaaa actttattta aatccgtttc cagttgattg atataattta
     3661 tatcttcaga gagtttccac atgattagag ctccttcata taaattaaaa actcttctag
     3721 taagatcagg aatttttaaa gtaggattaa gttttttatt ttttcttaat tctaaaaagt
     3781 atttattgga gatagtctcc catattttca caaactcttt aaaatcttgc ttaaaaaaat
     3841 cattttcact tggaagctgt gcacaaaaaa gagcaatagg acaaccctgg taaaaacctt
     3901 tttcttcttt gatttgcttt ttgattgcag agcataaatt ttttgcaaat aaatcaggtg
     3961 tcttactttt tcttaagatt tgcctaagcc aaaaaaggac atccgcacaa aattttcgaa
     4021 ggaccgctct acctaagata tcttttgatg gataataatc ataaaaacta gcctttgcag
     4081 ttttagattc ttgaataatt tggttgattc ctgtatttac atacccctgt tcataaaaaa
     4141 gtttagttgc tactattaaa attctctctc tcggacccat tgcaatacaa taaaaaaaaa
     4201 tattctttta tcaataaaaa aatagacaaa tctgtctgta ttaaaaaaat aattaacaga
     4261 caagtctgtc tgtattagga gcgatttatg aaaacttttc atcaaacgga aattccagtt
     4321 ctatggagtc atttagattc aaatcatcat gtaaataatg caatctttca attttacttt
     4381 gatgaagcta ggatgcaagc gctagagtct acaggtttta acttggcaga acttagaaaa
     4441 ataaatgtag gacctgttat ctataaatca gaaattgaat ataaaaagcc actctatcat
     4501 cccgaaactg caatcattca aacttggttt gagggcttag aaaaagtgag agggatcacc
     4561 tgccaaaacc tatatcgaaa gtcagatgga gaattggttt gcaaggctcg tttctattcc
     4621 attttctttg atctagaaaa agataaacct tggaaaatcc cactcgaatt gggcgaaaat
     4681 ttgagagagc tttcaggacc ttctaaaaat actttagcag cgtaaaaaat ccgttaaggg
     4741 attgtgccaa ataaatttct agatgaaaaa gattctacga aagcagttgg ctttgtgatt
     4801 caatcgttta gcgaattctt tgcacaatcc taagtattaa taggcatttt tattgatcaa
     4861 accagtaaag ggagttagta aaattatttt taaatcaaag aagatagacc agttctctat
     4921 atagtaaata tcagcttcta tccttttttc aatggatgta tctcctcgca aaccatttac
     4981 ctgcgcccaa ccagtaatac cagcctttac cgcatgacgt ctcatatatt gtctatgttc
     5041 atttttaaat ttttcaacaa agaatggcct ttctggtcta ggacccacaa cagacatatt
     5101 tcctgctaat acattaaaaa actgaggtgt ttcatctatc gacaatttac gaagaatccc
     5161 tccaattgga gttaccctag gatcattctt agtagtccat attgtatcag aatctgtctt
     5221 tgcttgtgta tacatagttc taaatttgag cataaaaaaa cttttattat caagacctac
     5281 tctctcctgg cgaataaaaa tatctccttc agaagtcagt ttaattaaaa tcgaaatgat
     5341 aaggtagaca ggagaaaaaa gaattataaa aaacaaggca aaaacaaaat caaaagatct
     5401 tttaataaaa cgattgtagc ccatccgcat aggaatatct cgaatagaaa taattggaac
     5461 accgtccatt ccctctactc ttcctttcgc tgcaataaac tcaataaact caggaatgat
     5521 ttttaattca atattctcat gatcacaaat atctatgatt tcacgtaggt tttcactatc
     5581 cgcaaattct ttggcgtaaa taagaagatc tggtttatat tctaacagaa ttttctcaat
     5641 atcctttaga tttccaatga ttttaaaatc acttcttgta tcagacttgg aaatactctt
     5701 tatataaccg actatattat aaccataaat tttatgtcct tggataacat ctgttatccg
     5761 ttctgctgtt tttccagtcc caatgatgat tacatttcgt aggttatatc ctctttccct
     5821 cagtcgcatc aatacaattc tgaaaatgta atgaacgagt gcagtaagta ggatattcgt
     5881 aacagcatag taggctacaa acaaacgaga aaagctctct cctctgactt ctcctctaaa
     5941 aaaaaacaat agagacaaaa taaaaagtaa atttaagatt acaccacttg caatcacaac
     6001 aagctcatca aaaaatgcga gccctctgcg tggatgataa agatcaatcg atataaatgc
     6061 taaaacctgt gtaatcgata aaacaataga aagaataata taattcgagg tattaaaata
     6121 aaaactctta tccaaaaagt atctaagtaa aaatgcgatc caaaaccctg aaaaggatat
     6181 cactaaatcc aatccaataa acattaactt aaaagtttgg tttctctctt ttaacattac
     6241 ggaccctccg cgcttatacc agaataccca ccgatacccg gatcgagtgg tctctttctg
     6301 aatctgtaaa tccttgctct tgtttgagcg gctgctggtt cttctggacc aagatttaca
     6361 ttgattaggt taattgaaaa gaagacagac tgatcgtaaa aactaactag actatctaag
     6421 gaggttcctc ccggtatagc tctcaaatcc atcgcataac caaccctata ctcaaaatta
     6481 tgaatgttgt atttgccaat caaaataaag cgattaacgt taaatgctgt cgtctgtctt
     6541 gcttgcgatc catttaaacc taaaccattt gcaatatctc tttggatcgt agtttgttga
     6601 aatgctgtat ttggacttgt atattgttgg taagtattgt cgatagttgc ctgatctgtt
     6661 ccatttctaa aaattgcatt ttgtccaatc tcatctgtaa atcgccatgg ctgagttacc
     6721 ctagaatcaa gttctaattc gattcctaag tttctagtaa cctgaacatc agtttgaatg
     6781 taaaatcgat aagaatctag gaaggaactt cttgtattgt agtattttcc tgcttggttg
     6841 taggttcctg catacactcc gaattgctcc aactgactat ttctaaaaac atgaaaccaa
     6901 gttccaccca cctcgaaatt tttaaaatta cgaataaatg gtaatgagaa tccacccatt
     6961 ttataagaaa cagttaggtt attatagaga ggactatttt gtgcagtatg atatacgtaa
     7021 tcattgttaa aaaagattcc agtgtaaaag cttcttcgtt tttcaagaag tgtttctctc
     7081 ttttttccaa atccatctat aaaatcaaaa aagacagctg ctctagccac tgtaaaatac
     7141 catctctgtt ggtttgtagg ctgtgggttg tattcacgag aaaactcacg aagatctcga
     7201 atcgttttaa tagaaaattc aacattttca aatgcgttac tttctaaagc aaattctaat
     7261 tctcgaacac ggtctcgtcc tctcataaga gtctgttctg gtaattcaga tcgactacca
     7321 cttgtccaac gatacgtaga tgacaataga agcgccggaa ctccaaaact taatttatga
     7381 ctttgtctgg tatagatata ggagttacga cgaagttccc gatctctaga taaatctgtt
     7441 gtttggattc cacccggaac atctaaagaa tatttttgaa acccataata agcagaagga
     7501 gaaaaagtca aatagcctcc aaaatttagg tttgtcttta aacctgtttc ggctaccgtt
     7561 tcaatttcag ttctagcaac ggatgcttta ggatcaccaa atggataaga acctgataag
     7621 cctggaataa ctccagatga aattgattgt gaattacgag caggacttcc ataaaatctt
     7681 tttaccacat tcgaaacgtt tatatcccag taaacaggtg aataaaaata aggaagcatt
     7741 cctacttgag atgaatttcg aatgatcgtt cttggtaact cctcatacaa aggataaaag
     7801 tctgaataca cagccggatt ataaggaagg tagaaataat ttcttctagc tgaaatattt
     7861 acagataagt ctccatgagt ttgggaataa tccaacgccc atgttgtatt ttgtctgata
     7921 aaaggatttc tctgctgtct tctagtatag agagattgca atgtatttgc tggctcatat
     7981 ctaaaaccaa attcatagtc atatcttgga tttgtataac gttcaaattg gatctgtaaa
     8041 tttctagttc catcccctgc gatattatta ttccgtgcat ttgctcttag attgattttg
     8101 ctccaatcct gttgttgcag tcctatattt cgaataggat aagaccaatt tggattattg
     8161 gcttgtaaaa cttggctagc agtcgctaaa cagtctgttc ttccgccagg taatttaagc
     8221 ttatcattat cataagcaca taaatctcct ttgtctactt gatttgtagc cattaaggtg
     8281 ctcacgtcgc ctatcgggtc ttttggaact ccatatctgc tttcataggt gtctaccgtt
     8341 ctataagatt tgaaattggc aagaccagtt tctaaattat agtttaacca aggacttgtc
     8401 ttccaaaaat caaatgcaat tgcttgccca gtaacttgat aaaaatctac tttcactctt
     8461 ctaccaactg gcataaatag agaaggaacg gctgttggat ctgaccattg ataatgtcct
     8521 tgaaagaaac ttccttgcga acgattcttt ccaaactgtg taacccaacc acttcctaga
     8581 ttagaactat aatacattgg aatccaaaac aattcggttc caccaacttg aaattttaag
     8641 ttagtcgcta taatgctatt atcgctgtaa aaaacgattc gcttcgcttt gaatttataa
     8701 tgaggaactt cagctccgca agctgtaaaa tgccccatat ccaaaacata tttattctca
     8761 tcaatttttt tgattttttt cccaacaaag aaagatggat atagagtcgc ttttgtatcg
     8821 tagacaactc ctctctccat tttcacatca tagataaatt tttcaccctt cacagtaagt
     8881 gagttatcta cgaactcaac atttccttct gcatagattt catttttttt tgagtccaca
     8941 aaaattgaat ccgcaattaa attaccttgg cctattttta attttacttt tccccgaaga
     9001 attaaaactc ctgactttgt tttatctaca gataaaagtt ctccctcact tgcattttca
     9061 ataataaaag agtcttgctt tttatttgct tctttctgca aattaaactc ttcttttact
     9121 ttttcgtctt ttggaacaac agcctctctg agccttgctc tctttgcata aatcgaacct
     9181 tctatactta atcccatcgc acgaagatac tcatctactt cacggtcaga taaaatatct
     9241 aaaacctttc ggactaattt cttttgtgtt tccgaaattc taaattcttt gattgtatcc
     9301 gcttctgttt ttttaggttc atttccatct acattgattt ggcgtatcag ctctgttgta
     9361 tcttgagaaa atattgaaat ggataaagaa aatagtaaaa gtacaaaata agatcgaaaa
     9421 actcgcactc tgcggtaaat acttattagc cttactgatt tcttattttg catgaaaatt
     9481 taattttgta gtaatttttt aaaaataatt catttctatt tgaattattg tatttgcact
     9541 ttgaagagta atttttgcat cttcgtattt aggcggacct agctttgatt taacgttatt
     9601 agatacaccg taaccctctt tagggattcc aattaaattt gtatccattt tcaaattatt
     9661 gttttcatca tgcataattc caatggcata ttcaccacaa ggaatatctt taaaatgaaa
     9721 ggttgccata ttggaatgaa ttggtaattt ttgggattga aatgctttat cgcgcttact
     9781 aggaaagcca tcgcttttat taaataaatt gattaatgct tgcccatcat tatttctaaa
     9841 tttaataatc tctactttta atgagcatag atcttctcct gcaagtgcaa aggaataaat
     9901 aaaaatgagt aagagcatga ttcgtttaaa gaacattcgc ctttcctaaa ttaagttaga
     9961 tttttcaatc gataaattga aaaggttctt tatgtttttt gaatcatttc acagttgccg
    10021 tcgaatacca ctccatcggc aatttgtagc tttgctgttt taatatttcc aacaacttta
    10081 ccagtagact gcatttcgag tctttcggtc gcaattacat ttcctgtaac atgtcccgcc
    10141 acaacaacgg tatgtgcctt gatatttgct cgaat
//
LOCUS       DAOQOS010000166        10172 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_3475_length_10172_cov_5.405654, whole genome shotgun sequence.
ACCESSION   DAOQOS010000166 DAOQOS010000000
VERSION     DAOQOS010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 10172)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10172)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10172
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3475_length_10172_cov_5.405654"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(402..1148)
                     /locus_tag="PLF66_23280"
     CDS             complement(402..1148)
                     /locus_tag="PLF66_23280"
                     /inference="COORDINATES: protein motif:HMM:NF013964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HMZ67030.1"
                     /translation="MEKNKKKSYYNEKWFAFSDLVKKRDEKCLKCGRKEPFVILQVHH
                     RVYKPGLEPWEYPLSDCITLCKGCHAREHKLIEPNDGWTLISITDREESDGVCERNGC
                     GAEIRYEHLTYHPKWGYKIVGSTCSEHLTEKEQFLGKKVVEIFEKTSNFIDNSIWKKD
                     VTKSGVEFIYTTHKFNQIRIYGNESNYSFQILIKQKGRNWFDYQKIINTKNLSLERVK
                     ELGYIVLKGFTTKDEDEKELLRNIYRRIKK"
     gene            complement(1162..1677)
                     /locus_tag="PLF66_23285"
     CDS             complement(1162..1677)
                     /locus_tag="PLF66_23285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67031.1"
                     /translation="MIRKLIDHRREIEKNKDAYTFDLKNDRLVQTFLNHIKKDYEGIR
                     FNESKKDILKNIILQIFQAQDNRPFFHVEGTELPLCWNRPADWNCNYIKYEWGHLLSK
                     NQSVGKYNLIENLGLYSARCNQHIQSSMNIQELMVYGGLIAQRISNVLTQRRKLFESN
                     HWKELLNKIEE"
     gene            complement(1966..2415)
                     /locus_tag="PLF66_23290"
     CDS             complement(1966..2415)
                     /locus_tag="PLF66_23290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67032.1"
                     /translation="MQFDVGPVYRWDKFLYKQGGGSIKPRWLIYLGNSGIIAEPKLHL
                     FCTTTTSYFPERKDRAKLFPELDYSSIFTNDCYLYFDEKINHLYDYQIEPHKKDIEFK
                     GELRDKDLNEIYLGYWIYKISPIFLTYIKNSLENKGILNLPTPKKNI"
     gene            complement(2405..2950)
                     /locus_tag="PLF66_23295"
     CDS             complement(2405..2950)
                     /locus_tag="PLF66_23295"
                     /inference="COORDINATES: protein motif:HMM:NF024671.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4065 domain-containing protein"
                     /protein_id="HMZ67033.1"
                     /translation="MDWFEKQKLDNVVSFFAEKHKELTNKNLSQTFLYKYLAFLDFTY
                     LKKVGHPSLNLKYLAMERGPVPIDIYKNKNNLKSELYEFENIKDDIYIVHSKRKADLD
                     YLDSEEIKIMRALLNKYAHDYATASEISEDSHKEIKAWIKAFNVKKNQPMKYEDELEF
                     SENNLTFEEEAFKVWQSLHAI"
     gene            3297..3668
                     /locus_tag="PLF66_23300"
     CDS             3297..3668
                     /locus_tag="PLF66_23300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67034.1"
                     /translation="MQLLFMFLFLISSVVILYDNYSHSILTNQLPWGHIFLNLVGISL
                     IFVPIKLGMDLFAKIMSFMAFGICLLYELELRKYSPETIEVYINFALWVFLKALICYL
                     LYFSAKNIVLLSEGVDGRKRD"
     gene            3649..3888
                     /locus_tag="PLF66_23305"
     CDS             3649..3888
                     /locus_tag="PLF66_23305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67035.1"
                     /translation="MEEKEIKKIKSYIQDIEQNKKKMLELFHSFSEKYSYRKMAIILE
                     INHSHINKIMNSERNVSIETLGDYILKILDYKESE"
     gene            complement(4061..4276)
                     /locus_tag="PLF66_23310"
     CDS             complement(4061..4276)
                     /locus_tag="PLF66_23310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67036.1"
                     /translation="MMKPSPNKEYPLRSHEKMWLLAPSRLVVEVKIFSLFVRLMQVQR
                     LHSCHETCGKEQVCVPSVQSQDIASIC"
     gene            complement(4273..6618)
                     /locus_tag="PLF66_23315"
     CDS             complement(4273..6618)
                     /locus_tag="PLF66_23315"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HMZ67037.1"
                     /translation="MNNYRYELLNDKEFELLARDLLQNEFKIKMQSFKKGKDKGIDAR
                     YSGSSDNEIIMQAKHYSNFNNLKSEISKEIVKVGNFNPLPLKYFLFTSLDLSVKELDE
                     IFDIGKIYFKSKDNIYYKTKIDNLLSAKKNNYIEEKYYKLWINSSNILKRILHNAEHG
                     SSDFYEKKILKSSSLYVTTKYFNLALKKLKDNKFLIISGEPGVGKTTLAHNIIYKFLA
                     KGFKLIYIDAYISEAEKLLSKDPKERQIIFFDDFLGRYIDNITNPKNTDSKIINFIDR
                     IKGSKNKYFILTSRTVFLNSAKLISEKLNNSGLEFSEYELKLNEYNLYDKALILYNHL
                     YFNDSTRQYLNFVKSDNFYHKIINHINYNPRIIEFITKLETFKKCNSRDSYIEYVLKC
                     LDEPALIWDHAYKNEIDYEAKALLDTIFTLGRYASEEELVKKTFFSRLDYFKNSKNIS
                     TSFDSYNKSIRTLQNSLIITQRDIEKNTLKFILINPSLGDYLISFYNKSPEEKVGLIK
                     SCISMHQIGNNFHPNKKGFINLSEKEIAIFKDKVILEYKELAHNSEYSYSSPEAYLIQ
                     ILLDSFEFVDIRRDIYNLFKKLKIQEISANHFFYILKFLSKERELGVLKYIRRNILSL
                     CSIFLESISDSDEIVLLKALFDNAHIDFKEFIEKNDLKESYIAKIEECIKSRIDSALE
                     DSDFLDNDDLFNYYGKLDYDIAQSNFEDRLNRIIDEICNDIDENLEYSWADFTSRSDI
                     ESSISAIVDSWKTSENKINYVAHVNVNSEKNLIEKVEDLFT"
     gene            complement(6886..7833)
                     /locus_tag="PLF66_23320"
     CDS             complement(6886..7833)
                     /locus_tag="PLF66_23320"
                     /inference="COORDINATES: protein motif:HMM:NF027488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4868 domain-containing protein"
                     /protein_id="HMZ67038.1"
                     /translation="MISNSKEIFSQLSLLEFSKFTLEFWIIKEHKNKRESIYEAFFVM
                     IDDSFREKIIQVVNNIFKNKNFILKEYSHEVIDNEEEIMFIDSKETEISQIIEKTLDK
                     ESDEKKVKDINELSDTWAYLIKLSHKNSEINFFKKLNRKWKIEKSKKEFFYSFFKNKM
                     ITALKEEDVIQISKTFDYIDYNGIIYILNKRNFETALNFRKGMEKNRDSLITELNELS
                     IVENLDDFKEAIGNNLHLLRKASMIKKSQYYKDENFMKKLMEVNKKNKWGLTFKNNRI
                     VFNKEKDKIEEILSCLNDDRLESPITLSIYDVQGKRKVQ"
     gene            complement(7830..8099)
                     /locus_tag="PLF66_23325"
     CDS             complement(7830..8099)
                     /locus_tag="PLF66_23325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67039.1"
                     /translation="MNIDINKPVELTVFLFFLMILCFLSIKTQIVFINPILAVLGYRL
                     YDVKFNTRGKPKNRIILSRYSIEQDTYYDVSVLSEYMLITHRRIQ"
     gene            complement(8362..9969)
                     /locus_tag="PLF66_23330"
     CDS             complement(8362..9969)
                     /locus_tag="PLF66_23330"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HMZ67040.1"
                     /translation="MNKILYYGDNLPVLKTHFQNESVDLIYLDPPFNSKANYNILFED
                     KAGKDSAAQVGAFEDTWHWTEETERIYQEVVETAPADVVDMISAFRKFIKKNDMLAYL
                     VMMTIRLVELRRILKSTGSIYLHCDPTASHYLKIVMDTIFGVKNFRNEITWIRTAAHS
                     DAKNYANVSDIILYYVKTNNYTFNSIYKPYDEKYLKKYYKHKDENGRVFLDRDLTAGG
                     LSGGGYNYEWKGVKKIWRCPIETMEKYEREGLLYYTSNNTPRLKQFLDEMEGVPVNNV
                     WEDIPPINSQAAERLGYPTQKPRALLERIIKSSANKDSIILDPFCGCGTTISACESLN
                     KNEGYNLTWLGIDITHLAINLIKGRLYGEYNINPKKDYQLVGEPKDLDGAVALSEQDR
                     YQFQWWALSLVNARPFGDKKKGSDKGVDGLIYFIEYPENKTEKAIVQVKSGKVKSGDI
                     RDLKGVVEREEAAFGIFITLQEPSRDMTEEAASSGFWKNRPEFPKIQILTIENILNGS
                     KVRIPHQVYHSKEAHKVEKKDTTGKLF"
BASE COUNT         3265 a         1647 c         1375 g         3885 t
ORIGIN      
        1 cggatgcccg ctaaatttta aaattaaatt gggttgagaa aatggcaact aacgtacaag
       61 agtagctgac gttcggaaaa atgttcaaaa atgcgggcag cattttttga acattttccc
      121 gaatgtctcc gagccacagc taataggagc aagacctgtg actgaatgca agaaaaattt
      181 gaatttgtta gcaaattttt ctggaatgaa ggcgcaggcc ttgcaaatgt tatacaaatt
      241 caataaaaag ttccgctcaa acaactatag ctggctcgga gcaagaccct gaatcagttc
      301 tcagggtcgc agtagctact ctattgttgt gcgcattttt ttcataattt aaatttagtt
      361 aatttccaat tagcatattt accaatttgt aagaacagta tttatttttt aattcttctg
      421 taaatatttc taagtaattc tttttcatct tcatctttag tagtaaagcc ctttaaaact
      481 atatatccaa gttcttttac tcgttccagg cttagatttt ttgtatttat aattttttga
      541 taatcaaacc aatttctacc tttttgtttt attaatattt ggaaagaata attactttca
      601 tttccataaa ttcgaatctg attaaattta tgtgtagtat aaataaattc tactccactt
      661 ttagtgacat cttttttcca aatagaatta tcaatgaaat tactagtttt ttcaaagatt
      721 tcaacaactt tcttacccaa gaattgctct ttctcagtta aatgttctga gcatgtactg
      781 ccaacaattt tataacccca ttttggatga taagttaagt gctcgtatct tatttctgct
      841 ccacacccat tcctttcaca gactccatct gattcttctc tgtccgtaat tgaaatgaga
      901 gtccaaccat cattaggctc aatcaatttg tgctctcttg catgacaacc tttgcaaagt
      961 gtaatgcaat cacttagtgg atattcccaa ggttctagac ctggcttgta aactctatgg
     1021 tgcacttgaa gaattacaaa tggttctttg cgcccacact ttaaacactt ttcatctctc
     1081 ttcttaacta gatcagaaaa agcaaaccat ttttcgttat aatatgattt tttcttattc
     1141 ttctccatta ttttaattag attattcctc tatcttgttt agtaattcct tccaatggtt
     1201 tgattcaaat aattttctcc tttgagtaag aacattagat attctttgtg caatgagtcc
     1261 accatatacc attaactcct gaatgttcat agaactttgt atgtgctggt tacatctcgc
     1321 tgaatataaa ccaaggtttt caatcaaatt atatttacca acactttgat tcttagacag
     1381 taaatgaccc cattcatatt ttatataatt gcaattccaa tcagctggac gattccaaca
     1441 tagcggtaac tctgtcccct caacatgaaa gaaaggtcta ttatcttgag cttgaaaaat
     1501 ctgtaatata atgtttttta atatatcttt cttcgactca ttaaaacgga taccttcgta
     1561 atccttttta atatgattca aaaatgtttg aactagccga tcattcttta aatcaaaagt
     1621 atatgcatct ttattttttt ctatttctct gcgatgatct attaattttc ttatcaattt
     1681 ttaaatcctt tgtagtaata aaattttata gagcttgaaa aaattgcgca caacgtttca
     1741 gcatatgaga cgttttgggg ctggcgcaat ttttgcctct tcatgcaaaa acttgtgaca
     1801 gacacaaaat gtggcgaagc ccgaacaact gaatcttttc tcagttgtga agctcatcat
     1861 gctttgttag gcgatcgttt tattttcgtg aactcctgca aagtctaact gacgactaca
     1921 tcgaaaacca gcctcgacac gaggtcgagt gtattaataa ttattttaaa tatttttttt
     1981 gggtgtaggc aaattaagaa tccctttatt ttctaaacta ttttttatat atgtaagaaa
     2041 aataggactt attttataaa tccaatatcc aagatatatt tcgtttaaat ctttatctct
     2101 taattcacct ttgaattcaa tatctttttt atgtggttca atttgataat cgtataagtg
     2161 atttattttt tcatcaaaat aaagatagca atcatttgta aaaatagaag agtaatctaa
     2221 ttctggaaag agtttagccc tatcttttct ttccggaaaa taagatgttg tagtcgtgca
     2281 aaataaatgt agttttggtt cggcaataat tcctgaattg ccaagataaa taagccatct
     2341 aggcttaatc gaccctccgc cttgcttata aagaaactta tcccatctgt aaacaggtcc
     2401 aacatcaaat tgcatgaaga gactgccata ccttaaaagc ttcttcctca aatgttaaat
     2461 tgttttctga aaattcaagt tcatcttcat atttcatagg ttgattcttt tttacattaa
     2521 atgccttaat ccaagcttta atttctttat ggctatcttc gcttatttct gatgcagttg
     2581 cataatcatg agcatattta tttaagagcg cgcgcattat ttttatttct tctgaatcta
     2641 gataatctaa atctgctttt ctttttgaat gaactatata aatatcatct ttaatatttt
     2701 caaattcata caactcgctt ttaagattgt ttttattttt gtatatgtct attggaacgg
     2761 gacctcgttc cattgctaaa tatttaagat ttaaggaagg gtgtcctact tttttaagat
     2821 atgtaaaatc caaaaaagct agatatttat aaagaaaagt ttggcttaaa tttttattag
     2881 taagttcctt atgcttttcg gcaaagaaag atactacatt atctaatttt tgcttttcaa
     2941 accagtccat agtatccttc ttttattttc atcttataac tacacatata tttagttttt
     3001 aatctgctat tagtcaacca taaataaatc aaaaaaggga ttactcaata aaataaatgt
     3061 tgacaaaata gtgtccataa agtatggtaa atataggagg taataaaaat gataccattt
     3121 gatacaaagt ttttgcttga aattggtttg atagcagtag cggtcgtagc ctacgctgca
     3181 attcaatact acttagagca aaaaaagaag taagacttct ccctgccccg aaaggggtag
     3241 ggtcatttgg ttatcatttt tattcccttt aactattaga ctggataagg ttttgtatgc
     3301 aattactatt tatgtttcta tttttgattt cctcagtggt tattctatat gacaattata
     3361 gccatagcat tttaactaat caacttcctt gggggcatat atttttaaat cttgtaggca
     3421 tttcacttat attcgttcct ataaaactcg gtatggattt atttgcaaaa attatgtctt
     3481 ttatggcttt cggaatatgc ctcttatatg aattagagct tagaaaatat tctccagaaa
     3541 caatagaggt atatattaat tttgctctat gggtttttct taaagctctt atctgttatt
     3601 tactatattt cagtgcaaag aatattgttt tacttagcga gggagttgat ggaagaaaaa
     3661 gagattaaga aaattaaatc ttatattcag gatattgaac aaaataaaaa gaaaatgcta
     3721 gaactctttc attcttttag tgaaaaatat tcttatagga aaatggctat tatccttgaa
     3781 ataaatcatt ctcatataaa taaaattatg aatagtgaac gtaatgtatc tattgaaaca
     3841 ttaggagact acattttaaa aattcttgat tataaagaat cagaataagt ctttcgagac
     3901 acggatgtcg agaaacctac ttctaaaaat tttagagtca gtttcttcaa aagcgtggtc
     3961 gaaaataaaa tgtcgcctaa cgttaagagt atccgacgtt gagaaaatgg gaagaaaatg
     4021 cctcaagcat ttttctttcc attttctcaa tgtctccgag tcaacaaata ctagcaatgt
     4081 cctgtgactg aacggatggc acgcaaactt gctcttttcc gcaagtttcg tgacaggagt
     4141 gaaggcgctg gacttgcatt agtctgacaa ataaactaaa tattttcact tctactacta
     4201 agcggctcgg agcaagaagc cacatcttct cgtggcttcg cagtggatac tctttgttag
     4261 gcgaaggttt catcatgtaa ataaatcttc aactttttca attaaattct tttcagaatt
     4321 cacattaaca tgtgcaacgt aattaatttt gttttcagaa gttttccagc tatcgactat
     4381 tgcactaata ctagattcta tatcacttcg actagtaaaa tcagcccatg agtattctaa
     4441 attttcatca atatcattac atatttcgtc aataattctg tttaatcgat cctcgaaatt
     4501 tgattgagct atatcataat caagcttacc atagtagtta aataaatcat cattgtcaag
     4561 aaaatccgaa tcttctaagg cggaatctat tctgcttttt atacattctt ctatctttgc
     4621 aatataagat tcttttaaat catttttttc aataaattct ttaaaatcga tatgggcatt
     4681 atcaaataaa gctttaagta aaacaatttc atcactgtca gaaatgcttt ctaagaatat
     4741 agagcataga cttagaatat ttctcctaat atattttaaa acgcccaatt ctctttcttt
     4801 tgaaagaaat tttaatatgt aaaaaaagtg atttgctgaa atttcttgaa tttttaattt
     4861 tttgaataaa ttatatatat ctctcctaat atcaacaaat tcaaaactat caagtaaaat
     4921 ttgaatgaga taagcttcag gagacgaata ggaatactca gaattatgag caagttcttt
     4981 atactctaat atcactttgt ctttaaatat agctatttct ttttcagata aatttataaa
     5041 tccttttttg ttagggtgaa agttgtttcc aatttggtgc attgagatgc aagattttat
     5101 tagtccaact ttttcctcag gtgatttatt ataaaaactt attaagtagt ctcctaatga
     5161 tggatttatt agtataaatt ttaatgtatt tttttctata tctcgttgag ttataatcaa
     5221 gctattttgc aaagttctta ttgacttatt gtaagaatca aaactagtcg aaatattttt
     5281 actattctta aaataatcta atctagagaa aaaagttttt ttaactaatt cttcttcaga
     5341 ggcatatcgc cctaatgtaa aaattgtatc tagcaatgct tttgcttcat agtcaatttc
     5401 atttttataa gcatgatccc aaattaaggc tggctcatca aggcatttca agacatattc
     5461 tatatagcta tctctgctat tacatttttt aaatgtttct aatttcgtaa taaactcaat
     5521 gattcgggga ttataattta tgtggttaat tattttatga taaaaattat cactttttac
     5581 aaaattcaaa tattgtctag tagaatcatt aaagtataga tgattatata aaatcaaagc
     5641 tttatcatat aaattgtatt catttagttt taattcatat tccgaaaatt ctaatcctga
     5701 gttattcaat ttttcggaaa ttaattttgc actatttaaa aataccgttc gactcgtcag
     5761 gataaaatat ttatttttac tacctttaat tctatcgata aaatttataa ttttagaatc
     5821 tgtatttttg ggatttgtta tattatcaat atatcttcca agaaaatcat caaagaatat
     5881 tatttggcgt tcttttggat cttttgataa taatttttca gcttctgaaa tgtaggcatc
     5941 tatataaatt agtttaaacc cttttgctag aaatttataa attatattat gtgctaaagt
     6001 tgtcttgcca acaccaggtt ctcctgaaat tattaaaaat ttattatctt ttaatttttt
     6061 aagggcaaga ttaaaatatt ttgttgtcac atataatgaa gagcttttta atatcttctt
     6121 ttcataaaaa tctgatgaac cgtgttctgc attgtgtaat attcttttga gaatattgga
     6181 actgtttatc cagagcttat aatatttttc ttcaatataa ttattttttt ttgctgataa
     6241 taaattgtct attttagttt tgtaataaat attatcttta gatttaaaat atatttttcc
     6301 aatatcgaat atttcatcta attcttttac agataaatcg agacttgtaa acaaaaaata
     6361 ttttaatggt agaggattga aatttcctac ttttactatc tctttactta tttcagattt
     6421 taaattatta aagttagaat aatgcttagc ttgcattata atctcattgt cactacttcc
     6481 tgaatatctt gcatctatac ccttatcctt tcctttctta aaactttgca ttttaatttt
     6541 aaattcattt tgaagtaaat ctctagctaa aagttcaaat tctttatcat ttagtagttc
     6601 atatctataa ttattcaaga atttattcct cattcaataa agtttgatga aactttcgcc
     6661 taacgttcaa tggtagctga cgttttggtg aaggcgtgaa tcttgcgttt gtttgcaaga
     6721 ttcatgactg aatcaaaatg tggtgaaacc caagccaccg aatcttctct cggtggcgca
     6781 gcagctaacc tttgttaagt gcccgttttt tgcttctaca actaaaaagt tagccccata
     6841 ttttttataa ccagcctgag acacggatgt cgaaggctgt ttttgctatt gcacttttct
     6901 ttttccttgt acatcataaa tagacagtgt aatgggcgat tctaaacgat catcatttaa
     6961 gcaggataag atttcttcta ttttatcttt ttccttatta aaaacgattc tgttattttt
     7021 aaaagtcaaa ccccacttat tctttttgtt cacttccatt aattttttca taaagttttc
     7081 gtccttgtag tattgacttt ttttaatcat tgaagctttt cttaatagat gtaaattatt
     7141 tcctattgct tctttgaaat catctaaatt ctcaacaata ctaagttcat ttaactctgt
     7201 aattagagaa tctcgatttt tttccatacc ttttctgaaa ttcaatgcag tttcaaaatt
     7261 tcgtttattt aaaatataaa tgattccgtt ataatcaata taatcaaatg ttttactaat
     7321 ttgtattaca tcttcttctt taagggctgt aatcatttta tttttaaaaa aactataaaa
     7381 aaactctttc ttggattttt cgattttcca ttttcgattt agctttttaa aaaaatttat
     7441 ctcactattt ttgtgtgata attttattaa gtatgcccac gtatccgaaa gttcatttat
     7501 atcttttact tttttttcgt ccgattcctt atctaaggtt ttttcaatta tttgtgatat
     7561 ttcagtttct ttactatcaa taaacataat ttcttcttca ttatcaatta cttcatgcga
     7621 atattctttt agtataaaat ttttattttt gaatatattg tttacgactt gaattatttt
     7681 ctctctaaat gaatcatcta tcattacaaa aaaggcttca taaatagatt ctcttttatt
     7741 tttatgctct ttaattatcc aaaattctaa agtaaactta gaaaattcaa gtagggataa
     7801 ttgggaaaaa atttctttag aattggaaat cattgtattc tcctatgtgt aattagcata
     7861 tattccgata agacagatac atcatagtaa gtatcttgtt caatcgaata tcttgacaat
     7921 ataattctat tttttggttt tcctctagta ttgaacttaa cgtcatatag ccgataacca
     7981 agaacagcaa gtattgggtt tataaagaca atttgtgttt ttattgagag aaagcataaa
     8041 atcattaaaa agaataaaaa aacagtaagt tctactggct tatttatatc aatattcata
     8101 aaagctacta aataaggtaa tgtatagttt aataaatcga aagacttgta agatacatct
     8161 gtaacattta tttggaattc tactttaatt tttgttaaga gataaaaaag aattattaat
     8221 ggaaaaattg atgaaaacaa gaacacaaaa tttaaacagg aaaaataatt cgagttatag
     8281 aaatttttta ttacaaacaa aatgaataaa ggagaataac cacttacaaa aataaaaatt
     8341 tcaattaaaa tacgaagcat ttcaaaataa cttcccggta gtatcctttt tttcaacttt
     8401 atgagcttct ttcgaatgat aaacttgatg aggaattctt actttacttc catttagtat
     8461 attttcaata gttaatattt gtattttagg gaattccggt ctgtttttcc agaaaccaga
     8521 agaggcggct tcttccgtca tatctctact tggttcttgc aatgtaataa aaattccaaa
     8581 tgcagcttct tctctttcca caacaccttt caaatctcta atatcaccgg acttaacttt
     8641 tccacttttc acttgaacaa tcgctttttc tgttttattt tcgggatatt ctataaaata
     8701 aatcagaccg tcaacccctt tatcagaacc tttctttttg tctccaaagg gtcttgcatt
     8761 tactaaagat aaagcccacc attgaaattg atagcgatct tgctcactca aagcaactgc
     8821 tccgtctaaa tcttttggct caccaacaag ttgataatct tttttaggat ttatattata
     8881 ttctccatat aatctacctt tgataagatt tatagcaaga tgagtaatgt caattccaag
     8941 ccacgttaaa ttatagcctt catttttatt caaactttcg caagcagata ttgtagttcc
     9001 acatccgcaa aaaggatcaa gtattattga gtccttattt gcagaactct tgataattct
     9061 ttctaataaa gctcttggct tttgtgtagg ataaccgagt ctttctgctg cttgtgaatt
     9121 aataggcgga atatcttccc atacattgtt aacaggaaca ccttccattt catctaagaa
     9181 ttgctttaaa cggggagtgt tattagatgt ataatacagc agtccttccc tttcatattt
     9241 ttccattgtt tcgataggac aacgccatat ttttttaact cccttccatt cataattgta
     9301 tccaccgcct gatagtccgc ctgccgtcaa atccctatca agaaaaactc ttccattttc
     9361 atctttgtgc ttataatatt tctttaaata cttttcatca taaggcttat agatactatt
     9421 aaatgtatag ttattagttt ttacgtagta taagattata tcagacacgt tcgcataatt
     9481 tttagcgtca ctatgagctg ctgttcttat ccatgtaatt tcgtttcgaa aattttttac
     9541 gccaaaaatt gtatccatta caatttttaa ataatgacta gcagtaggat cgcaatggag
     9601 ataaatgctt ccggtagatt ttaaaattct tctcaattct acaagccgaa ttgtcatcat
     9661 tacaaggtag gcaagcatat catttttttt gataaacttc ctaaatgcag aaatcatatc
     9721 cacaacatca gcgggtgctg tttccactac ttcttggtaa attctttcag tttcttctgt
     9781 ccaatgccag gtatcctcaa atgctccgac ctgtgcggcc gaatctttac ctgctttgtc
     9841 ttcaaaaaga atgttgtaat tagcttttga attaaagggg gggtcgagat aaatcaaatc
     9901 aaccgattca ttctgaaaat gagtctttaa aacgggtaaa ttatccccat aatacaaaat
     9961 cttattcata gtgtcattaa atattatgtc cctaattttt caacaattca aattaaaaaa
    10021 tttttagttt tgtccgagac acggatgtcg aggacaccaa taaaaaggaa atttatgggg
    10081 cggtattagg actaagccta aagcaaaaaa tggcacttaa cgttttgcgg cttggcgtag
    10141 tggcggattt ttagcacaaa agatcaatcg aa
//
LOCUS       DAOQOS010000167        10089 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_3523_length_10089_cov_6.413992, whole genome shotgun sequence.
ACCESSION   DAOQOS010000167 DAOQOS010000000
VERSION     DAOQOS010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 10089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10089
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3523_length_10089_cov_6.413992"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..794
                     /locus_tag="PLF66_23335"
     CDS             <1..794
                     /locus_tag="PLF66_23335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67041.1"
                     /translation="SKDFSIEERNFMNVTLLTTIEKNRILSSGEASSSIWKDIPLNGT
                     SLKAVVIRSFLDQTYLIRIAGREIRINSEAKLFEGERLELKATKESNRLELEILNRSF
                     GENEIADEIQKKAWSTRFKNPTEMIRNTLDTTKFDLSTHRIFQILNAYFPGVHWRSET
                     NYFEWKLEDGEGSAYFDKNKNGNGFYLHLTLNFLGNIDTYLNWKEEDLSDLSIHILFD
                     KLESYRIAYEKLGILKKMLSSNSIPVNHIIFHYSTMDQVKGDWRV"
     gene            794..1057
                     /locus_tag="PLF66_23340"
     CDS             794..1057
                     /locus_tag="PLF66_23340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004460664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HMZ67042.1"
                     /translation="MNAVALKFIPEKDNAPRVIASGSGFLGDKIHQIAELNQVPILQD
                     ETLASSLLQVPVGKEIPEALYKSVAVVFSFLYKLENELKSRQA"
     gene            1091..2251
                     /locus_tag="PLF66_23345"
     CDS             1091..2251
                     /locus_tag="PLF66_23345"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HMZ67043.1"
                     /translation="MKVIKISDLKVGSKFTKPLYLDKDSIFINANTAVSEADIKRLNK
                     FGFKEVLTSGELIKEDSPEIVLDTNTFPTTEHDFKLLQLKNIHLQIEKSIPSFEAVFK
                     DSFIVMQALFRKISEDKPFDINPVRDVADKLVDHVKANNHLCYCLIQHTPSGYYLYNQ
                     VVYATLFALLIGHLLEYSRPRMIDLAISGLLADIGMAKIPSIISEKNATLTEDEFKVI
                     KKHPLFGYQILTKVLKLKNALGLVALQHHENFDGTGYPQRIQKKEIDEFARVFTIADN
                     FAAHILDRPWRKALLPYEAMKNMISLNTNKYDLTYIRLFLNKVAMYPVGSWVELSDAS
                     KAIVIDSNPNKPLRPSLMVVKNSNGVKIKDSTFINLSEEDKIYITKAIPSDY"
     gene            2255..3412
                     /locus_tag="PLF66_23350"
     CDS             2255..3412
                     /locus_tag="PLF66_23350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67044.1"
                     /translation="MSFFILADSEVWYVWPETNAYTVFQVVLWVFIFYLIVQAGKYIF
                     LRKKTTSSSWTHILNYAYNKKLSSKEITVLEHFFNTLDMSVNQKANLLADKKTFQSLL
                     YTYLKKYHSDSVETYVRILDKLFPTLDHHLEIKSILDVHVGEVCAAEFDGLYFLARVM
                     SKNDTEILLSVNDPAFRKDREGKEVNLYFYRDTLGGFLIPGVVRKFNGNGFVFNQQGD
                     VQTKGEEHLVAELTRDIELFPWGVKTTDSTLSLNDTQSNVLNTIDEVVNEETESKTEE
                     GSSDKETEKDSEKDSLSMRGTMIRISDRAMLINVKDPFFNEAVLRKIDIWEMTVGLTP
                     GEQPLVLKGKIMRSDLFSESFIFKYVAPSESDMKQIFDEIKKHSPIRGFIS"
     gene            complement(3409..3645)
                     /locus_tag="PLF66_23355"
     CDS             complement(3409..3645)
                     /locus_tag="PLF66_23355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67045.1"
                     /translation="MLQENVENIPIVHLSCKLFEEMIYDIKRIKRSRKAPFYALNRTN
                     VAKNNLNQELLLDLFILSRRTRVTFIVKSMKRKS"
     gene            3973..4314
                     /locus_tag="PLF66_23360"
     CDS             3973..4314
                     /locus_tag="PLF66_23360"
                     /inference="COORDINATES: protein motif:HMM:NF014119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HMZ67046.1"
                     /translation="MEKVTWGKVGEDKASKYLEEKGFQVLERNFRNRLGEIDIIALEK
                     ETIYFIEVKNWKGNLKLPLEIFTKKKIGIMRKLAEYFFYTHPKFNQLFFVSFCLIEIR
                     DDKINFYKNLF"
     gene            4364..4567
                     /locus_tag="PLF66_23365"
     CDS             4364..4567
                     /locus_tag="PLF66_23365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002770880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67047.1"
                     /translation="MNKVIHSTGTLIDPADFGPDPIILRKRGISRNLQDKYDLYKRKI
                     EDPGYMEFAINKIAVELTHFLSK"
     gene            4568..5251
                     /locus_tag="PLF66_23370"
     CDS             4568..5251
                     /locus_tag="PLF66_23370"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HMZ67048.1"
                     /translation="MSLSKKTILNTVKLLAFDVDGTLFSSEEIILDTYIEAIQRFIQK
                     HNKKIPIPTKEKIIEQIGLPVKKIFLNLLPELEEIERDEISDSVLILLREKIQEKKGK
                     IYEGVEDTIKELHKKGYVLCVASNGRIGYIEAILQTYHLMSYFEAIVVIDYEKIKSKG
                     DIVASYLQHYNLKGENSLMIGDRKSDLDAALDNGSHFAYCEYGHAEPNEIQQFSIQIK
                     SIRELLDYL"
     gene            5476..6387
                     /gene="lpxC"
                     /locus_tag="PLF66_23375"
     CDS             5476..6387
                     /gene="lpxC"
                     /locus_tag="PLF66_23375"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017859380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HMZ67049.1"
                     /translation="MESKIYRKTLKSQIVISGIGLHSGKKVSLKLIPAEANTGLIFIS
                     NVKNKKSIIPVSLNHVVDTSNAVTLGDGFRIVQTVEHLMAALSTRGITDLIMEIDSSE
                     IPIMDGSSQPFLEAIDSAGIIESNELIEPIKIANPIWVVDGDKYIVILPSETLKVTYN
                     IHFNHPLLRGQSITIDLDESTMAKEILPARTFGFLRDVEALQARGLALGGSLDNAIVL
                     SDEGYLNESLRFENECIRHKVLDLIGDLSIVGRPIVGHIIASKAGHALDVSMGKLVMS
                     RITGDEISKFKSKRIHDFRERPQVAAK"
     gene            6495..8210
                     /gene="ptsP"
                     /locus_tag="PLF66_23380"
     CDS             6495..8210
                     /gene="ptsP"
                     /locus_tag="PLF66_23380"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HMZ67050.1"
                     /translation="MPTNKTVIYKGIPASYGKFYGKALKIISSKHIILQTRINEKKVK
                     FEISKFQKALTKTKKELEGIIEKKTVNQDIKDILVSQICMLDDPLLIDGVIRKISDLH
                     QNAALALFNSIEEISNKFTTLNDDYFSERAVDIRDIGRRIEDNLLGRKSDYNILSDIK
                     EEVIIVAHELTPSQMMHIDKKMVKGIATETGGKTGHMAILAKNYSIPTVVGINNITSV
                     IEDNEFILIDADDGVVIRNPVLNQIKLYAASSSIEPEKYDNLSSVTHDGQRILVKVNL
                     DTENDCDSILRLNADGVGLYRSEVILIEDPDKIYDEEEQFHIYKKIASDMHNFPVTIR
                     TFDLGGDKFDTKYREDNPFLGNRGIRFALKNTSWFKKQLRAILRASVYGNISIMIPMV
                     SSISEVIETKKLIVDAKMELRRKGIDYKDVNVGIMVETPACALALDQFAKECDFFSIG
                     TNDLLQYTMAVDRNNHYISELYNPYNLSFLRLLKMIIGASNTHQIPLSICGELASDTK
                     FTILLLGLGLRELSVSLPLVRKIKKIISSTDISQAQILAEKVLNLSENEKFVEIDAFL
                     FNRHI"
     gene            8225..8824
                     /locus_tag="PLF66_23385"
     CDS             8225..8824
                     /locus_tag="PLF66_23385"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002147524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction ATP-dependent DNA helicase
                     RuvA"
                     /protein_id="HMZ67051.1"
                     /translation="MISGLKGKLERLDVNLAEINVSGVIYEVNISFKSFEEIKTNLGK
                     EVHLYIYHLINERTQKLFGFLNLRDKELFKLVRGLHGIGEMTALKILSFMSADELYRA
                     VVNGDNSQLEKIPKVKGKTSEKIIFEVKQNLKKFEAFLNTEKENYIPTKEEKQDLSVL
                     ALIQLGFDEKTAIKEVAKVVSSRKIEDTAEIIREVLKLS"
     gene            complement(8924..10057)
                     /locus_tag="PLF66_23390"
     CDS             complement(8924..10057)
                     /locus_tag="PLF66_23390"
                     /inference="COORDINATES: protein motif:HMM:NF013625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HMZ67052.1"
                     /translation="MLRNFSLKVILVSIVLAIFVACSSSPKKDAKDAPKDASTKDAKD
                     VSKDASKDASKDAKSTAPASKRSTDDTASTEKEKVGLRSADESLTKKLGSLPETPPGA
                     KPGQCWIPVVTPATYKEITENVVKRPSGEKIETLPARYETVTEQKLVKEATTVVETVP
                     AVYETQEEKIVIRPAGKRIVQIPAVYETVEEKVVDKEGYTEWKKDSTTGITCLVETPP
                     TYKTVQKEVVKVPASSKEELVPEETAIVTKQVVKTPATTRTVEIPAEYRTVMIQKLVE
                     PEKEIRVATPAEYQQVTKKVVDQPEKAEWREILCENNTTKEKVSEIQKALKVAGFDPG
                     STEGSVDDKTMKALNDYQKNKSLPVDEGKHINMITVRSLGISK"
BASE COUNT         3428 a         1525 c         1830 g         3306 t
ORIGIN      
        1 catcgaaaga cttttcaatt gaagaaagaa attttatgaa cgttacattg cttactacaa
       61 tcgaaaaaaa tcgaattcta tcttctggtg aagcatctag ctcgatttgg aaagacattc
      121 ctctgaacgg aacttctcta aaggcagtcg taattcgttc ttttttagac cagacctatt
      181 taattcggat agccggtcgg gaaataagaa tcaactctga agctaagcta tttgaaggtg
      241 aaagattaga attaaaagct acaaaagaat ccaatcgact ggagctagaa attttaaatc
      301 gatcctttgg agagaatgaa atcgcagacg aaatccagaa gaaagcttgg tcgactcgtt
      361 ttaaaaatcc aactgaaatg attcgaaaca ctttggatac aaccaaattt gatttatcaa
      421 cacacagaat cttccaaatt ctaaacgcat acttcccggg agttcactgg agatcagaaa
      481 caaactactt tgaatggaaa ctagaagatg gagagggaag tgcctacttc gataaaaata
      541 aaaacgggaa cggattctat ttgcatctca ccttaaattt tctaggcaat attgatacgt
      601 atctaaattg gaaggaagaa gatctttccg acctatccat tcatatactg tttgataaat
      661 tggagtctta tagaattgca tatgaaaagt tagggatttt aaaaaaaatg ctttcttcaa
      721 attcaatccc tgtgaatcat attatttttc attattccac aatggatcaa gttaaagggg
      781 attggagagt ttaatgaatg cagttgcatt aaagtttatt cctgagaaag acaatgcgcc
      841 aagggtgatt gctagcggtt ctggatttct tggagataaa attcaccaaa tagcagaact
      901 gaatcaagtt cctatactac aggatgaaac tttagcttcc tctttattac aagttccagt
      961 gggaaaagaa ataccagaag ctttgtataa atctgttgcg gttgtgttca gctttttata
     1021 taaattagaa aatgaattga aatcgagaca agcttgagat attgttctga atggagagtt
     1081 tttgcaatca atgaaagtca ttaaaatatc cgatttgaaa gtaggaagta aatttacaaa
     1141 gcccctatat ttagataagg atagtatttt tattaatgcg aatactgctg tttcagaagc
     1201 agatatcaag cgcctaaata aatttggatt caaagaggtc ctcacaagtg gagaactcat
     1261 aaaagaggat tcacctgaaa tcgttttaga tacaaatact tttcctacaa ctgagcatga
     1321 ctttaagctg ctccaattaa aaaatattca cctacaaatc gaaaaaagta tacctagctt
     1381 cgaggcagtt tttaaagact cctttatcgt aatgcaagct ttatttcgaa aaatctcgga
     1441 agataaacca tttgatatta atcctgttag agatgtggcg gataaacttg tagatcatgt
     1501 aaaagcgaat aatcatttat gctattgttt gattcagcat actccttcag gctattatct
     1561 ttacaatcaa gttgtgtatg caacgttatt tgcattattg attggacatc tacttgaata
     1621 ctctcgtcct aggatgatcg atctagcaat ctctggacta ctcgcagata ttggaatggc
     1681 aaaaattcca tctatcatat cagagaaaaa tgccacatta acagaagatg aattcaaggt
     1741 tattaaaaag catcctttgt ttgggtatca aattttaaca aaggttttaa aactcaaaaa
     1801 tgcgttaggc ttagtagctt tgcagcatca tgaaaatttt gatggaactg gatatcctca
     1861 gaggattcag aaaaaggaaa tagatgagtt tgccagagtc tttacgattg cagataattt
     1921 tgccgctcat attttagata gaccatggag aaaagcactt cttccctatg aagctatgaa
     1981 gaatatgatt agcttaaata caaataaata tgatctaact tatattcgct tgtttttgaa
     2041 taaagtagcg atgtatcccg ttggatcttg ggttgagcta tcagatgcat ccaaagcaat
     2101 cgtgattgat tctaatccta ataaaccact tcgtccttct ctgatggttg tcaaaaatag
     2161 caatggagta aaaattaaag attccacttt tatcaacttg tccgaagaag ataaaattta
     2221 tataacgaaa gcaattcctt ccgattatta aagtatgagt ttctttattc tagctgattc
     2281 tgaggtttgg tatgtatggc ctgaaacgaa tgcctatacg gtatttcagg ttgtcctttg
     2341 ggtattcatt ttttatttaa ttgtgcaagc tgggaagtat atttttcttc gaaagaagac
     2401 aacaagctcc tcttggacac atattttaaa ttatgcctat aacaagaaat tatcttcaaa
     2461 agaaataaca gttctagagc atttttttaa tactctagac atgtcggtca atcagaaagc
     2521 aaatctttta gcggataaaa aaacttttca aagcctatta tatacgtatt taaaaaaata
     2581 tcattctgac tcagtggaaa catacgttcg tattttagat aaactttttc caactctcga
     2641 tcatcatcta gaaattaaat cgattttaga tgtccatgta ggggaagtct gtgctgctga
     2701 atttgatgga ctctatttct tggcaagagt catgagcaaa aatgatacgg aaattctact
     2761 ttctgtaaat gatcctgcat ttagaaaaga tagagaagga aaagaagtga atctatattt
     2821 ctatcgagat acattaggtg gctttttaat tcctggggtc gttagaaaat ttaatggcaa
     2881 tggatttgtt tttaatcaac aaggggatgt tcagaccaag ggagaagagc atctagttgc
     2941 tgaacttaca cgcgatattg aattgtttcc ttggggagtc aaaacaactg attctactct
     3001 tagtctaaac gacactcaat ccaatgtttt aaatacgata gatgaagttg taaatgagga
     3061 aacagagagt aaaacagaag aaggtagttc cgataaagaa acggaaaagg attcagaaaa
     3121 ggattcttta agtatgagag gaactatgat tcgaatttct gatcgagcta tgttaatcaa
     3181 tgtaaaagat ccgtttttta atgaggcagt ccttcgaaaa attgacatat gggaaatgac
     3241 ggtcggactt actccagggg aacaaccatt ggttttaaag ggaaagatta tgcgatctga
     3301 tttattttct gaatctttta tctttaaata tgttgcacct tctgaatcag atatgaagca
     3361 gatttttgat gaaattaaaa agcattctcc gattcgaggc tttatctctt aggattttct
     3421 tttcattgat tttactataa aagtcactct agttcttcga gagagaataa atagatccaa
     3481 gagaagttct tgattgaggt tgttttttgc tacgttagtt ctattcaatg cataaaaagg
     3541 agctttccta cttctcttga ttctttttat gtcgtaaatc atttcttcaa agagtttaca
     3601 cgataaatga acaatgggaa tgttttctac attctcttgt aacaagccta gctccattcg
     3661 gaattctact atcgcctaaa atagtttatt aattgtttca ttagaaagat ggaatagaat
     3721 ccatttggtt ttggagcatg ccctcttttt ctttttttga aatctcaata gagaacggtt
     3781 ttgcaatgat tcgtttttta ttctaaattc tttttatagc tttgcaaagc atttaaaaag
     3841 taattatatc ttatcggctg tttatgtcgt ttaattagat attttacaat tttaaaaata
     3901 atcattcgaa aaaaaaatat tcatccgata aattagtaga taatcttaag tctaaatgat
     3961 ttgagatcaa tcatggaaaa agtaacttgg ggcaaagttg gagaggataa agcttcaaaa
     4021 tacttggaag agaaaggttt tcaagtatta gagagaaatt ttcgcaatcg tcttggtgag
     4081 atagatatta tcgcactaga gaaggaaact atttacttta ttgaagtcaa aaactggaaa
     4141 ggtaatttaa aactacctct tgaaattttt acaaaaaaga aaataggaat tatgcggaag
     4201 ttggctgaat atttttttta tacgcatcca aaattcaatc agttattttt tgtttctttt
     4261 tgtttaattg aaatacgaga cgataaaatt aatttttata agaatttatt ttagttagtg
     4321 agttttcaag gatgaaaact aaatttgtta cagggaagta actatgaata aagtgataca
     4381 ttctacagga acgttaatag acccggctga ctttggtccg gatcctatta ttcttagaaa
     4441 gcgaggtata tcccgaaatc ttcaggataa gtatgacctg tataaaagga aaattgaaga
     4501 tcctggatat atggaatttg caatcaataa aattgcagta gagttaactc actttctttc
     4561 taaatagatg tccttatcaa aaaaaacaat cctgaatact gtgaagctat tggcttttga
     4621 tgtggatgga actttattct catccgaaga aattatttta gatacatata ttgaagcaat
     4681 tcaaagattt atacaaaagc ataataagaa aatccctatc ccaacgaaag agaaaatcat
     4741 tgaacaaatc gggcttcctg tcaaaaaaat atttctaaac ctattgcctg aactagaaga
     4801 aattgaaaga gatgaaatct ccgatagtgt tctcattctg ctacgagaaa aaatccaaga
     4861 gaaaaaaggt aagatatatg agggagtaga agatacaatc aaagaattgc ataaaaaggg
     4921 atatgttctt tgtgttgctt ccaatggaag aatcgggtat atagaagcaa ttttacagac
     4981 ttaccatttg atgtcttatt ttgaagcgat tgtggtcatc gattacgaaa aaattaaatc
     5041 caaaggggat atagtagcat cttatttaca acactataat ttaaaaggtg aaaactcatt
     5101 gatgatagga gatcgaaaat ctgatttaga tgctgctttg gacaatggga gccattttgc
     5161 ttattgtgaa tatggacatg cagagccaaa tgagatacaa caattctcga ttcaaatcaa
     5221 atcaatccga gaattactag attatctcta aaacacttct aatcagtctt tccatttcta
     5281 cttaaatggg agtaattgta tcgatttgta atctaaatga atctctgttg tatagatgat
     5341 acagtgttgc gaaattgaca caataaattc gaaaaaatag gttttttagc ctcaaaaacc
     5401 cttgtaaatg tcataaaaat cactattttt actggtacgc tgattgcata tgagtagtat
     5461 aaggaacaag aggatatgga atcgaagatt tatagaaaaa cattaaaaag tcagatagtg
     5521 ataagcggaa ttgggttaca ttctggcaag aaagtatcat taaaattaat tcctgcagaa
     5581 gcaaatacag gcttaatttt tattagtaat gtaaaaaata agaaatcgat tataccagtt
     5641 agtttaaatc atgttgtgga tacaagtaac gcagttactt tgggtgatgg ttttagaatc
     5701 gttcaaacag tagaacatct tatggctgct ctctccacca gaggcattac cgatttaatt
     5761 atggagattg actctagtga aattcctatc atggacggat cttctcagcc tttcttagaa
     5821 gcaattgatt ctgctggaat cattgaatcc aatgaactca ttgaacctat taaaattgca
     5881 aacccgatct gggttgtaga tggagacaaa tacattgtta tccttccatc tgaaacatta
     5941 aaagtaactt ataatattca ttttaatcat ccattactcc gcggacaaag tataactatc
     6001 gatctagacg aaagtactat ggcaaaggaa atcctacctg ctagaacgtt tggcttttta
     6061 agagatgtag aagctttaca agctagagga cttgcacttg gtggctcact cgacaatgcg
     6121 atcgttctct ctgatgaagg ctatttaaat gaaagcctaa gatttgagaa tgaatgtatt
     6181 cgtcataagg tccttgactt gattggtgat ttatcgatag ttggaagacc aatcgtaggt
     6241 catattattg cctcaaaagc aggtcatgct ttagatgtat caatgggcaa attagtaatg
     6301 agtagaatca ctggggatga aatctctaag ttcaaaagta agcgtattca tgattttagg
     6361 gagagacctc aggttgcagc aaagtaggta agacagtttc ctattctctg aaacctgctc
     6421 taaataataa ttttagcaga gttaaataat aatggaaatt ttttaaggaa ttcgtatggt
     6481 ttactaagta tactatgcct acgaataaaa cagtcatata taaaggaatt cccgcttcct
     6541 atggaaagtt ctatggcaaa gctctaaaaa taatttctag taagcatatc attttacaaa
     6601 caagaattaa tgaaaaaaaa gtaaaatttg aaatttccaa atttcaaaaa gctctcacaa
     6661 agacaaagaa agaattagaa ggtatcattg aaaagaaaac tgttaaccaa gatattaaag
     6721 atatcttggt ttcacagata tgcatgttag acgatccact attgatagat ggtgtcattc
     6781 gaaaaatcag tgacttgcat caaaatgcag ccttggcttt atttaactcc attgaggaaa
     6841 tttcaaataa atttacaact ttaaatgatg actactttag cgaaagagct gtcgatatac
     6901 gcgatatcgg gagaagaata gaagataatc tacttggaag aaaaagtgat tataacattt
     6961 tatctgatat aaaagaggaa gttattattg tagcacatga actaacacct tctcaaatga
     7021 tgcatataga caaaaagatg gtaaaaggaa ttgctacaga gacaggaggt aagacaggtc
     7081 atatggccat tcttgctaaa aattactcca ttcctactgt tgttggtatc aataatataa
     7141 cttctgtgat agaagacaat gaatttattt tgattgatgc cgatgatgga gtcgttattc
     7201 gaaatcctgt tttaaatcaa attaaattgt atgcagctag ctcttctatt gagcctgaaa
     7261 agtatgataa cttatcttcg gtcacacatg atggtcagag aatactagta aaagtgaatt
     7321 tagatacaga aaatgactgc gactcaatat tgagattgaa tgcagatgga gtaggtcttt
     7381 atcgtagcga agtgatttta attgaagatc cggataaaat ttatgacgaa gaggagcagt
     7441 ttcatattta taaaaaaatt gcatccgata tgcataactt ccctgttaca attagaactt
     7501 ttgatttggg tggtgataag tttgatacaa aatatagaga ggataatcca tttctaggaa
     7561 atagaggaat tcgttttgca ctaaaaaata cttcctggtt taaaaaacaa cttagagcaa
     7621 ttcttagagc ttctgtctat ggaaacattt ctattatgat tccgatggtg agttctattt
     7681 ccgaagtgat tgagactaaa aaattaattg tagatgctaa aatggagcta cgtagaaaag
     7741 gcattgatta caaagatgtg aatgttggaa tcatggttga gacacctgct tgtgctttag
     7801 ctctcgatca atttgcaaaa gagtgtgatt ttttttcaat aggaacaaat gatttacttc
     7861 aatatacaat ggcagtagat cgaaataacc attatatctc agaattatat aatccttata
     7921 atttgtcttt tttaagacta ctcaaaatga tcattggtgc atccaatact catcagattc
     7981 ctctcagtat ttgtggtgaa ttagcatctg atacaaaatt tactatacta cttcttgggt
     8041 tagggttacg tgaattgtca gtatcccttc cactcgttag aaaaataaaa aaaattattt
     8101 catctactga catttcacaa gctcaaatac tagcagaaaa agttctcaat ttatcagaga
     8161 atgaaaagtt tgtggaaata gatgcatttc tatttaatag acatatataa attaaggaaa
     8221 gaaattgata agcgggttaa aaggaaaact agagaggcta gatgtaaatt tagcagaaat
     8281 aaacgttagt ggtgttatat atgaggttaa catttctttt aagtcttttg aagaaataaa
     8341 aactaattta ggaaaagagg ttcatctcta catttatcac ttaattaatg aaagaacaca
     8401 aaaactattt ggttttttaa atttacgaga caaggaatta tttaaattgg tgcgtgggct
     8461 ccatggaatc ggggagatga ctgctttgaa gatcttgtct tttatgagtg ctgatgaatt
     8521 gtatcgagcg gtcgtcaatg gagataattc acagttagag aagattccta aagtcaaagg
     8581 aaaaacatct gaaaaaatta tctttgaagt aaaacaaaac ctgaaaaagt ttgaagcctt
     8641 cttaaataca gaaaaggaaa attatattcc aacaaaagaa gagaagcaag acttgtccgt
     8701 tttagctttg attcaacttg ggtttgatga aaagacagct atcaaggaag ttgcaaaagt
     8761 tgtatctagt cgaaagattg aagacacagc agaaataata cgggaagttt taaaactatc
     8821 ctgatttcag ttttacaatc taagaaaagg agataggttc catcctgagg gcaaaaaaaa
     8881 tccgagttag aaactcggat tttttttaga aggtgttttt taattattta gagatgccta
     8941 aagagcgaac agtaatcata ttgatgtgtt taccttcgtc tacaggtagg cttttgtttt
     9001 tctgataatc atttaaagct ttcatagttt tgtcgtctac agatccctct gttgaacctg
     9061 gatcgaatcc tgctactttc aacgcttttt ggatttcact cactttctct tttgttgtat
     9121 tgttttcaca aagtatctct ctccattcag ctttctcagg ttgatctaca actttttttg
     9181 taacttgttg gtattctgca ggtgttgcca ctctgatttc tttttctggt tctactagtt
     9241 tttgaatcat tactgttctg tattcagcag ggatttctac agttcttgta gttgcaggag
     9301 ttttcacaac ttgttttgtt acgattgctg tttcttcagg aactaactct tcttttgaag
     9361 aagcaggaac tttcactact tctttttgaa cagttttgta tgtaggtggt gtttctacta
     9421 aacaagtaat accagttgta gagtctttct tccattcagt atagccttct ttatccacta
     9481 ctttttcttc tacagtttca taaacagcag ggatttgaac gattcttttt cctgcaggac
     9541 gaatcacaat tttttcttct tgtgtttcat aaacagcagg aactgtttct actaccgttg
     9601 tagcttcctt tactaatttt tgttctgtta cagtttcata acgagctggc agggtttcta
     9661 ttttttcgcc agatggtctt tttactacat tttcagtgat ttccttatag gttgcagggg
     9721 tcacaactgg aatccagcat tgacctggtt ttgctccagg aggagtttct ggtaatgagc
     9781 ctaatttttt agttaaactt tcatcagcac ttctcaaccc aactttttct ttttcagtag
     9841 atgcagtatc atctgtgctt cgtttagaag caggcgcagt ggattttgca tccttagagg
     9901 catcctttga agcatctttg gatacatctt tcgcatcttt tgtgctggca tctttgggag
     9961 catcttttgc atccttttta ggggagctac tgcaagcaac aaagattgct aagacaattg
    10021 atacaagtat aacttttaag ctaaaatttc ttaacatttt ttattctcca aaatatctat
    10081 tgtaaccat
//
LOCUS       DAOQOS010000168         9951 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_3592_length_9951_cov_5.263541, whole genome shotgun sequence.
ACCESSION   DAOQOS010000168 DAOQOS010000000
VERSION     DAOQOS010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 9951)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9951)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9951
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3592_length_9951_cov_5.263541"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(398..682)
                     /gene="cas2"
                     /locus_tag="PLF66_23395"
     CDS             complement(398..682)
                     /gene="cas2"
                     /locus_tag="PLF66_23395"
                     /inference="COORDINATES: protein motif:HMM:TIGR01573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HMZ67053.1"
                     /translation="MRDNWIISYDISDPKRLRKVAKFLEGYGERIQHSLFRVQANDRD
                     IEKIKFELTKKIAEEDSVFYLRFCNLCAQKVHKLNPKLDWKKEDVTFRIV"
     gene            complement(682..2310)
                     /gene="cas1"
                     /locus_tag="PLF66_23400"
     CDS             complement(682..2310)
                     /gene="cas1"
                     /locus_tag="PLF66_23400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020784107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-MYXAN CRISPR-associated endonuclease
                     Cas1"
                     /protein_id="HMZ67054.1"
                     /translation="MGLHSISYCERLFYLEEVEKILIADERVYEGRTLHEEIQIDNAD
                     INRVETFEYTSETIGLTGKVDRLQKRDGNWIPYEHKKGRSKSNGKNKDAWEPDIIQVT
                     GYALLVEEATGRKIEEARIKYHKDNVLVKIPITEELKEKTREKIYRAKELMSSVERPP
                     VAQNSNLCIRCSLAPVCLPEENRVIDESNYEAIRLFPPSREKETIHITGYKTSISKDG
                     DTIVVEKKNDYDSIEKIKFAVNEIESINIHGSAQISSQLIHYLMYQKIPVHWYTAGGS
                     YVGGLNFNPNNVQRKLRQYEALIDEKFKLYLAKKLTLAKSETQFKYLLRVSRTKKLNS
                     YEEEGKKQQIRNLLSEMNRVDSIDSLRGYEGSIARIYHGSIPSFLIDSVPKEMIPIGR
                     SKRPPKDRYNAVLSFLYSLLFRSVNQAIISVGIDPTIGFYHTPRSAAEPLVLDLMELF
                     RVTLCDMVLIGSVNRLMWDIEKDFLVTKEKVWLSDLGRKKAIQLYEERLDDKWKHPVT
                     NYSMSYYRMIELEVRLLEKEWTDKAGLFARARLR"
     gene            complement(2354..2488)
                     /locus_tag="PLF66_23405"
     CDS             complement(2354..2488)
                     /locus_tag="PLF66_23405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67055.1"
                     /translation="MNKPDMSPSAITLRIKRLAQLRKLCMSLKIAGEEEKKKEIETKS
                     "
     gene            complement(2481..2942)
                     /locus_tag="PLF66_23410"
     CDS             complement(2481..2942)
                     /locus_tag="PLF66_23410"
                     /inference="COORDINATES: protein motif:HMM:NF026257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HMZ67056.1"
                     /translation="MLDLYNEFKQIVNKFKENSIEYAICGGLAVTFYGYVRATVDIDM
                     LVLSEDMEESKKALREIGYSIEATPMELSAGKIHIERLTKVDKESGDYLPIDLMIVTD
                     SIRHIWQSRKQVQALDMEIQVVDKKGLIELKRMRNSAIDQEDILKLEESDE"
     gene            complement(2960..3421)
                     /locus_tag="PLF66_23415"
     CDS             complement(2960..3421)
                     /locus_tag="PLF66_23415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67057.1"
                     /translation="MRTVFLDLEKTIIHSWDDPVIINEVIVTERLTSIRPDLIGIYSF
                     AIYDDRDRNYFLHSMKQEIEDSFHLKIETSLVFTVQEIANTILKAQVKDIQTFIQTHG
                     KQNSFQEFIITKFKEGEFVLIDDLVENIEVSIDQLNLQFINPFSKTYSKLK"
     gene            complement(3418..3690)
                     /locus_tag="PLF66_23420"
     CDS             complement(3418..3690)
                     /locus_tag="PLF66_23420"
                     /inference="COORDINATES: protein motif:HMM:NF014273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HMZ67058.1"
                     /translation="MEYLNPEELEITHGVQEKKRFEELLTSIRDEGILETIKYVEYNG
                     LRYIVNGHHRHKIAMALQMKTVPVEQVYLPYNGYNTEDDLIYQDIR"
     gene            complement(3694..4416)
                     /locus_tag="PLF66_23425"
     CDS             complement(3694..4416)
                     /locus_tag="PLF66_23425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004755767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas5"
                     /protein_id="HMZ67059.1"
                     /translation="MEEFYIHVKAPFAAYRYFQAGNYRVTMPTMPHSAAYGLILNLAG
                     IEMRKGLNELTTLIKDVVREYSFQDENSVPSLKISIGDIDISKKGSVFQQLHDYPPQA
                     KEIQLKRAQGTKPRILPTRREFLLGLNTIIGVKSNTSGFIKRIFQGLSGESNYRYGLP
                     FAGDNNFLFDFIQIYEKAIYPANWYYPVSPDEIPETETTRLTVGIDRNDNSNTTSPLF
                     AITKEKTNTPPPNAWVWTPREG"
     gene            complement(4421..5293)
                     /locus_tag="PLF66_23430"
     CDS             complement(4421..5293)
                     /locus_tag="PLF66_23430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004778973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein
                     Cas7/Cst2/DevR"
                     /protein_id="HMZ67060.1"
                     /translation="MTTQNNKNLFCTVLTYAAPSSNYRGESEENRTVLQKISKNGKDY
                     AIFSSESIRNAIRETIAKKVSNDEINRTRLHNEGQLAVKFKAVPDAENYADDFLFGYL
                     VADTVSKKFDSVLRTNYAVALSPYKYEASFHQSPDIKDSPFKNQKSSVLLHKEISHTA
                     VQYPFALLGHDCRKNEKTIKWVKSLLDSIVELNNVAGGHARNLYDFTPKSIVVRLTPK
                     LVAGYDSYGFKEDGSWSELNRINENDLSKDEFYFAGEIVRTMKPEDKARLNSDHLFDN
                     PDKLIEKIKTDFLG"
     gene            complement(5311..7221)
                     /gene="cmx8"
                     /locus_tag="PLF66_23435"
     CDS             complement(5311..7221)
                     /gene="cmx8"
                     /locus_tag="PLF66_23435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004755853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-MYXAN CRISPR-associated protein Cmx8"
                     /protein_id="HMZ67061.1"
                     /translation="MKKQKSTDNEKLPPSKKSKNKKETIEEVKEITLEYKLHDLPSSQ
                     HKAGLAGLYLVIKWLNEQGESGKDILGKTEISETGLKVTLTKNDVKDLFDEIYKSEDV
                     EIISKSEPKKGDFQTFEKIEKTIFYFYETEAKSKRKDKETNVILEPTKEIIKKEKDKR
                     TGNIKDVKYYIYLDKFQEKKQDEEEEEIEPIKEEEFEDTITYYKYKMNFPKGSVLKGY
                     DKTPDSSWIKLWREAQWLLKGDRQRLIYENPYKEVEIIWEKFTKDKKENDLSKVLFLS
                     AQNNNAEKVDFKEINKNKFLLHFWVFVAQPYAYWTQRKDKKKYKTDEHGFIFAVPDIS
                     KLESFIFIFNDLIQSQDNEIKEYPIKRPEQAVVYLPGLAGLLLLKSIHDILKRKLNSN
                     YSDVVHGVDLFHYDFEWKSGKAKKNRASIFKGYTRIKPDIEFENKAAGIKKLYKNFFF
                     QKQMLQNLFEQRDELYNFYNLFSTREYEYFFKYWDGEFKPDAKTYFEEKKIKGEQEMS
                     DDGKIPKTIEPLVHQIVRTYVYSKMESKYQLVWDKDKKLLWSKAKNAPADDKDYENKS
                     KIAKDAFLAIRSRKDKDDFITYFTSTICSVSQYLGEEGYTLLAGSLYNENEALGWEKI
                     RALSMLALSANS"
     gene            complement(7218..9566)
                     /gene="cas3"
                     /locus_tag="PLF66_23440"
     CDS             complement(7218..9566)
                     /gene="cas3"
                     /locus_tag="PLF66_23440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004755739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase Cas3'"
                     /protein_id="HMZ67062.1"
                     /translation="MGKLFAKSKKEGREPLTLEKHSFDTEEAAILIFKKDSRFFQNWL
                     RFFKLDSSLEDKFLLNLRVACLFHDIGKANDDFQKAVDGKLTHQGIRHEHLSALILHL
                     PEVRVWLGQNGELDIPIITSAVLCHHLKATGINEHEYKWLVKPLRDYVILNLHHDEVK
                     NILKKVNSLLTGNLPIPELNFKSFNINDAIWKKVLQSGSKESGILGFKLDDKRKQFHL
                     ALKSALIVCDSASSGLVREEFLIIEWIPNVIHLDAIKENEIFIEIIQKRMNDKKIPEL
                     HEFQKQVATKGDRVLLMAGCGMGKTLAAWNWANEVSKKQKISKVIFLYPTRGTATEGF
                     KDYASWAPEADAALIHGTSEYELDSMFTNPEDPKAKNDYSIPEDQERLFALAFWSKKF
                     FSATADQLLSFLQNNYKGICLLPVLSECALIVDEVHSFDKAMFASLLSFLEHFDIPIL
                     CMTATLTPKNKQSLLDRGLKLYPTEEDRKEPEIEKLNELETHPRYNKTILDNAEQAKE
                     IATRSFKEGKRVLWVLNTVARCQEMYEMLSKELQTEVYCYHSRFKLSDRKNIHEKTVS
                     AFQGENQNSIAVTTQVCEMSLDLDADVLISEYAPVSSLVQRFGRANRHLKKGKDFRAE
                     LYLYKVNGSDKPYKPEQIKIAETFLKSLPEKDISQFLLATKLELKEFSAEEYKTLPSA
                     SFVDGGYYALSEAYRDIDEITVQAVLDEDLPEVLKILEENEKIRKEKLTDKKIKPIDG
                     YILPVPSKNKEINLRKEEHSILPKYLWVVSKKYYNEKRGFAL"
     gene            complement(9559..9795)
                     /locus_tag="PLF66_23445"
     CDS             complement(9559..9795)
                     /locus_tag="PLF66_23445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67063.1"
                     /translation="MKWNIQRKGRAWEKEEAWTKYELTPEKIEMFQGKIFWSDEQRLN
                     MIGLLLENVGIDTMLDLLEDLSLLKDAIRDRENG"
     gene            complement(9792..>9951)
                     /locus_tag="PLF66_23450"
     CDS             complement(9792..>9951)
                     /locus_tag="PLF66_23450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020779681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type I-MYXAN CRISPR-associated protein
                     Cas6/Cmx6"
                     /protein_id="HMZ67064.1"
                     /translation="YIRKTSRIHGKEIVGYPVLLTNLDPDESIKIQINGIGGRRKMGC
                     GNFVGIRV"
BASE COUNT         2841 a         1905 c         1446 g         3759 t
ORIGIN      
        1 agtggctcag gttgtggctt attgcgtgct caacgcctaa tggcatcaaa gataatttca
       61 ttaaatcaaa gattgtccaa tactatccgc agcctacggt gctcaacgcc taatggcatc
      121 aaagataatt tcatagcgga ttttttcgaa aggatataaa cgagtaaaac cctgcttctg
      181 attggtttta tttttccatt tcatttatct atctcctttt taaaatttta tttatatagg
      241 tttgaattca aatcttgttt cttaatattc aatcaatgtt ttcaatctag attcaggttc
      301 aagcatatcg atttataaaa atctagagat tatattgaat ttacatcatt aaaatctctt
      361 ttcctaaaat atgtcgctaa cttttataga tgcttgttta gacaattcta aatgtaacat
      421 cctctttttt ccaatctagt tttggattta gcttatgaac tttttgtgcg cataaattac
      481 agaatcgtaa ataaaaaaca ctatcttcct ctgcgatttt tttagttagc tcaaatttga
      541 ttttctcaat atctctatca tttgcttgca ctctaaaaag actatgttga attcgttctc
      601 catacccttc taaaaattta gcaactttcc ttaatcgctt cggatcgctt atatcatagg
      661 aaataatcca attgtctctc attatcgaag cctcgctctt gcaaataaac cggccttatc
      721 tgtccattct ttttctaata atcgtacttc tagctcaatc atcctataat aagacatgga
      781 ataatttgta actgggtgtt tccacttatc atctaatcgt tcttcataaa gttgtattgc
      841 tttttttctt cctaaatcag ataaccaaac tttttctttt gtaacaagaa aatctttttc
      901 aatatcccac ataagtctat tcacacttcc aattaaaacc atatcacata acgttactcg
      961 aaacaattcc attaaatcta atactaatgg ctccgctgcc gatcgaggag tatgataaaa
     1021 tcctatagtt ggatctattc caacggaaat gattgcttga tttaccgatc ggaaaagtaa
     1081 agaatataaa aaacttaaaa cagcattgta tctatcctta ggcggacgtt tacttctacc
     1141 tatcggtatc atttcttttg gaacagagtc gattaaaaag gaaggaatgc ttccatgata
     1201 aattcgagca atagaacctt cataacctcg gagcgaatca atagaatcaa cacgattcat
     1261 ttcagataat agatttcgaa tttgttgttt cttaccttcc tcttcataac tattcaattt
     1321 ctttgtgcgg ctaacacgaa gtaaatactt gaattgagtt tcactttttg caagagttag
     1381 ttttttggct agataaagct taaacttttc atcaatcaag gcttcatatt gtcttaactt
     1441 tctttgcacg ttattcggat taaaatttaa tcctcctaca taactgcctc cagcagtata
     1501 ccagtgaact ggaatttttt gatacattag gtaatgtatg agctgtgaag atatctgcgc
     1561 acttccatgt atatttattg attctatttc attcactgca aatttaattt tttcaatgct
     1621 atcatagtca tttttctttt cgactacaat cgtatcccca tctttagata tagaagtctt
     1681 atagcctgta atatgaattg tttctttctc acggctaggc gggaaaagtc taatagcttc
     1741 gtaattgctt tcgtcaatta ctctattttc ttctggaagg caaacaggag caagggaaca
     1801 tcgtatacaa agatttgaat tttgcgcgac cggcggtctt tccacagaag acattaactc
     1861 ttttgctcta taaattttct ccctagtttt ctccttcaat tcttctgtga taggaatttt
     1921 tactaatacg ttgtctttat gatatttaat tctggcttct tctatttttc tgcctgttgc
     1981 ctcttcgact aataaggcgt aacctgtaac ttgaataata tctggctccc atgcatcttt
     2041 atttttacca ttggatttag accgtccctt tttatgctcg taaggaatcc aatttccatc
     2101 tctcttttgt aaacgatcta ctttgccagt aagacctatc gtctcactag tatattcgaa
     2161 agtttcaact cgattgatat cggcattatc aatttgtatt tcttcgtgaa gagttcttcc
     2221 ctcgtagact ctctcatctg caattagaat tttttcgact tcttctaaat aaaaaagtct
     2281 ttcacagtag ctaatagagt gtaaccccat tacgcgaatt ggtggttgaa aatccgattc
     2341 catatttgaa tgcctagctt ttggtctcta tttctttctt tttttcttct tctccagcaa
     2401 tttttaagga catacataat tttctaagtt gtgccagcct tttaattcgc aaagtaattg
     2461 ccgatggtga catatcaggc ttattcatcg ctttcctcca attttaatat atcctcttga
     2521 tcgatagcac tattgcgcat tcgttttaat tcaattagac cttttttatc aacaacttga
     2581 atctccatat ccaatgcttg cacctgtttt cgactctgcc aaatatgacg gatagaatct
     2641 gtaactatca ttaagtcaat tggtaaataa tctccgcttt ctttatctac ctttgttaag
     2701 cgttctatat gaatctttcc ggcagatagt tccattggtg ttgcttctat tgaatatccg
     2761 atttcacgga gtgctttttt tgattcttcc atatcttcag acaataccaa catatcaata
     2821 tcgactgtcg cacgcacata cccataaaat gtcacagcaa gcccgccaca gattgcatat
     2881 tcaatagaat tctctttgaa tttgttaact atttgcttga actcgttata taagtctaac
     2941 atagattatc cgtaataagt tactttaatt tagaataagt tttcgaaaaa ggattaataa
     3001 attgaagatt taattgatca atagagactt cgatattttc tactaggtca tctattagaa
     3061 caaactctcc ttctttgaat tttgttatga taaattcttg aaacgagttt tgttttccat
     3121 gagtctggat aaaagtttga atatctttaa cttgcgcctt taaaatcgta tttgcaattt
     3181 cttgcacggt aaaaacaaga ctcgtctcta tcttcaaatg aaacgagtct tctatctctt
     3241 gcttcattga atgtaaaaaa taatttcgat ctctatcatc ataaatcgca aaggaataaa
     3301 tccctattaa atcaggtcta atggatgtta gccgctctgt tacaatgact tcattgatta
     3361 ttacgggatc atcccatgaa tgaatgattg ttttttctaa atctaaaaat actgttctca
     3421 tcgtatatcc tgataaatta aatcatcttc tgtattgtat ccattgtacg gtaaataaac
     3481 ttgctcgact ggcactgttt tcatctgcaa tgccattgcg attttatgtc tatgatgccc
     3541 atttacgata tatcgcaacc cattgtattc aacatacttt atggtttcca atatcccctc
     3601 atctctaata gaagtcaaca attcctcaaa ccgctttttc tcttgaactc catgcgtgat
     3661 ttctaattcc tctggattca aatattccaa attttatccc tctctcggtg tccataccca
     3721 tgcgttaggg ggaggagtat ttgtcttttc ctttgtgatt gcaaaaagag gagacgttgt
     3781 attactattg tcgtttctat ctatgcctac agtgagtcgt gttgtttcgg tttctggaat
     3841 ttcatctgga cttactggat aataccaatt agctggatag atagcttttt cgtaaatttg
     3901 aataaagtca aagagaaagt tattatcacc agcgaaaggt aaaccataac gataattact
     3961 ttccccactt aaaccctgaa agattctttt tatgaaacca gatgtattcg atttaacacc
     4021 tatgattgta tttaaaccaa gtaaaaattc tcgtcttgtt ggaagaattc taggctttgt
     4081 tccttgtgcc ctttttagct gaatttcttt tgcttgcgga ggataatcat gtagttgttg
     4141 aaaaactgag cctttctttg atatatctat atctccaatt gaaattttta aagaaggtac
     4201 tgaattctca tcttgaaaag aatattccct aaccacatct ttgattagtg tcgtcaattc
     4261 atttaatcct tttctcattt caattccagc taagttgagg ataagaccat acgccgcgga
     4321 atgcggcata gttggcatag tcactctgta gttacctgct tgaaaatagc gataagcggc
     4381 aaacggtgct ttaacgtgaa tgtaaaattc ttccattgca ttaacctaaa aaatcagttt
     4441 tgattttttc aatgagttta tcgggattat caaaaagatg gtctgaatta agtcttgctt
     4501 tgtcttctgg tttcatagtg cgtacaatct caccagcaaa ataaaattca tctttcgata
     4561 gatcattttc attgattcta tttaattcac tccacgatcc atcttcttta aaaccataag
     4621 aatcatatcc tgctactaat ttaggagtta gacgaacaac gatgctttta ggagtgaagt
     4681 cgtataagtt ccttgcatga cctcccgcta cattatttaa ttctacaata gagtctaaca
     4741 acgatttaac ccatttaatt gttttttcat tttttctgca atcgtgacct aatagagcaa
     4801 atgggtattg tacggcagtg tgtgagattt ctttatgtaa aagaacggag ctcttttgat
     4861 ttttaaaagg gctatctttt atatctggcg attgatgaaa agatgcttca tatttatacg
     4921 gacttagagc cactgcataa ttagttctta acacgctatc aaattttttt gaaacagtat
     4981 cagcaactaa atagccaaat agaaaatcat cggcataatt ttctgcatca ggaaccgctt
     5041 taaattttac agccaattga ccttcattat gaagcctagt tcgattaatt tcatcatttg
     5101 agactttctt ggctatggtc tccctaattg catttcgaat tgactctgaa ctaaatattg
     5161 catagtcctt tccattttta ctgatttttt gtagcactgt tctgttttct tcactttctc
     5221 ctcggtagtt ggacgatggt gctgcatatg taagaactgt acaaaaaagg tttttgttgt
     5281 tttgtgtagt cataaatttc tccttttatt ttaactgttt gcagataacg cgagcattga
     5341 tagtgcacgt attttttccc aaccaagagc ttcgttttca ttatataaag atccggccaa
     5401 taatgtgtat ccttcctcgc ctagatactg agaaacggaa catatagtag aagtaaaata
     5461 agtaatgaag tcatctttat cttttctact tcgtatagca agaaaagcat cctttgcaat
     5521 tttagattta ttttcataat ccttatcatc agcaggtgca ttctttgcct ttgaccatag
     5581 caatttttta tccttatccc aaactaattg atatttagat tccattttac tatagacata
     5641 agttcttaca atttgatgaa ctaatggttc tatagttttt ggaatttttc cgtcatctga
     5701 catttcttgt tctcctttaa tttttttttc ttcgaagtaa gttttcgcat ctggtttaaa
     5761 ctctccatcc cagtatttaa aaaaatattc atattctctc gtgctgaaca aattataaaa
     5821 attatatagt tcatctctct gttcaaaaag attttgtagc atttgctttt gaaaaaagaa
     5881 attcttatat aatttcttta tacctgcagc tttattttca aattcaatgt ctggttttat
     5941 gcgagtatat cctttgaaaa tagaagctcg atttttcttg gcttttccac ttttccattc
     6001 aaagtcataa tgaaataaat ctactccatg gactacatca gaataatttg aatttagctt
     6061 tctttttaaa atatcatgaa tacttttgag aagtagcaac ccagctaatc cgggaagata
     6121 aacaactgct tgctctggtc ttttaattgg atattcttta atttcgttgt cttgactttg
     6181 gattagatca ttgaaaataa aaataaagga ttctaatttt gaaatatcag gaacagcaaa
     6241 aataaatcca tgctcatctg ttttatattt ctttttatct tttctctgtg tccagtaagc
     6301 atagggttgt gcgacaaata cccagaagtg aagcaaaaat ttatttttgt taatttcttt
     6361 gaaatctact ttttcagcat tattattttg cgccgataaa aataaaactt tagacaaatc
     6421 attttctttc ttgtctttgg taaacttctc ccaaattatc tctacttctt tataaggatt
     6481 ttcataaatt aatctttgcc tatctccctt tagcaaccat tgtgcttctc gccaaagttt
     6541 tatccaagag gaatctggtg ttttatcgta cccctttaat acggaaccct tcgggaagtt
     6601 cattttatat ttatagtatg ttattgtatc ctcgaactct tcttccttga tcggctcaat
     6661 ctcctcctcc tcttcatcct gttttttctc ttgaaattta tcaagatata tatagtattt
     6721 aacgtctttg atgttcccag ttcttttgtc tttttctttt ttaataattt cttttgttgg
     6781 ttctaagata acgttagtct ctttatcttt tctttttgat ttagcttcag tttcataaaa
     6841 ataaaatatt gttttttcta ttttttcaaa agtttgaaaa tctccttttt tcggttcaga
     6901 tttgctaatt atctctacat cttcggactt ataaatctca tcaaacaaat cctttacatc
     6961 atttttagta agagttacct ttagccccgt ttcactgatt tctgtttttc cgagtatatc
     7021 ctttcctgac tccccttgtt cgtttagcca tttaataact agatacaacc ctgcaagtcc
     7081 agctttatgt tgagatgatg gaaggtcatg aagtttatac tccaaagtaa tttcttttac
     7141 ttcctcgatg gtctcttttt tatttttaga ttttttagac ggaggcaatt tttcattatc
     7201 cgtagatttt tgctttttca taaggcaaat cctctttttt cgttatagta ttttttactc
     7261 acaacccata aatatttggg taggatagaa tgttcctctt ttctaagatt gatctcttta
     7321 ttcttgcttg gaacagggag aatgtaacca tcgattggtt ttattttttt atccgtgagt
     7381 ttttcttttc taattttttc attttcttct aggattttta agacttcagg caagtcttca
     7441 tctaagactg cctgcactgt aatttcgtct atgtctctgt aagcctcgga aagtgcatag
     7501 tatccgccat ctacaaaaga agccgatgga agcgttttat attcttcagc agaaaattct
     7561 tttaattcta atttagtggc taagagaaat tgactaatat ctttttcagg aagacttttt
     7621 aaaaaagttt cggcaatttt tatttgctct ggcttgtaag gtttgtcact tccatttact
     7681 ttatataaat aaagttccgc tcgaaaatcc tttccttttt tcaaatgtcg attggctcga
     7741 ccaaatcttt gaaccaaaga agaaactggc gcatactcag aaattaaaac atctgcatct
     7801 aaatctaggc tcatttcgca tacttgtgta gtaaccgcaa tagagttttg attctctcct
     7861 tgaaatgcac tgactgtctt ttcgtgtata ttttttctat ctgataattt gaaacgacta
     7921 tgataacaat agacctcagt ctgtaattct tttgatagca tctcatacat ttcctgacat
     7981 cgagcaactg tatttaaaac ccaaagaact ctttttcctt ctttaaaact tcgagtagca
     8041 atttcttttg cctgttccgc attatctaaa attgttttgt tatatctagg atgggtttct
     8101 agttcattta atttttcaat ctctggttct tttctatctt cctcggttgg atataacttc
     8161 aaacctcgat ccaatagact ttgcttattc ttgggagtga gagttgctgt catacaaaga
     8221 attggaatat caaaatgctc caagaaacta agtagtgagg caaacattgc tttatcaaaa
     8281 ctatgcactt catcaacaat caaagcgcat tccgataaga cgggtaatag acatatacct
     8341 ttataattat tttgtaaaaa actaagcaat tgatctgctg ttgcactaaa gaattttttt
     8401 gaccaaaagg caagggcaaa taatctttct tggtcttctg gaatcgaata atcatttttt
     8461 gcttttggat cttctggatt tgtgaacata gagtctagtt catactcgga tgttccgtgt
     8521 ataagtgcag cgtcagcctc tggagcccat gatgcataat ctttaaagcc ttcggttgca
     8581 gttccgcgtg ttggataaag aaaaataacc ttactaattt tttgtttttt tgaaacctca
     8641 tttgcccaat tccaagcggc taacgtctta cccataccac aacctgccat gagtaaaact
     8701 ctgtcacctt tagtcgctac ttgcttttgg aattcatgaa gttctggaat ttttttatca
     8761 ttcattcttt tctgaatgat ttctataaat atttcattct ctttgattgc atctaagtgg
     8821 atcacattag gtatccactc tataattaaa aactcttctc taacaagccc agaggaagcg
     8881 ctgtcacaaa caatcaaagc agattttaat gctagatgga attgctttct tttatcatcc
     8941 agtttgaagc caagaattcc tgattcctta cttccagact gtaatacttt tttccaaatt
     9001 gcatcgttta tgttgaatga tttaaaattc aattctggta tcggcaaatt tccagtaagc
     9061 aaagaattta ctttcttcaa aatatttttt acttcatcat ggtgaagatt taggattaca
     9121 taatctctga gaggttttac aagccattta tactcatgtt catttatgcc cgttgctttc
     9181 agatggtgac aaagtaccgc tgacgtaata ataggaatat ctaattcccc attctgtcca
     9241 agccacactc ttacttccgg caaatgcaga attagcgcac ttaaatgctc atgtcgaatt
     9301 ccttgatgag taagtttacc atcaactgct ttttggaaat catcatttgc ctttccaata
     9361 tcatgaaaca aacaagcaac acgtagattc aaaagaaatt tatcttctaa cgaggaatct
     9421 aacttaaaga atcgaagcca attttgaaag aagcgtgaat ctttcttgaa aatgagtatg
     9481 gcagcctctt ccgtatcaaa ggaatgcttt tcgagagtta acggctctct tccttctttc
     9541 ttgctctttg cgaataactt acccattttc cctatccctt atcgcatcct taagcaaact
     9601 caaatcctca agcaaatcaa gcattgtatc aatgccaacg ttttcgagta agagaccaat
     9661 catgttaaga cgttgttcat cagaccaaaa gatttttcct tggaacattt caattttttc
     9721 cggtgttaat tcatatttag tccaagcttc ttctttttcc caagccctgc cttttctttg
     9781 aatattccac ttcatactct aatccctaca aaatttccac atcccatctt acgacgtccc
     9841 cctattccat tgatctgaat tttgattgat tcgtcaggat ctaggttggt aagtaaaact
     9901 ggatagccca ctatttcttt tccatgaatc ctggatgttt ttcgtatata t
//
LOCUS       DAOQOS010000169         9864 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_3642_length_9864_cov_6.334489, whole genome shotgun sequence.
ACCESSION   DAOQOS010000169 DAOQOS010000000
VERSION     DAOQOS010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 9864)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9864)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9864
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3642_length_9864_cov_6.334489"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..800
                     /locus_tag="PLF66_23455"
     CDS             <1..800
                     /locus_tag="PLF66_23455"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004423757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HMZ67065.1"
                     /translation="SFQTPRYKPVEYTKVNVVVRKQEQTNVALPDKKTIYAIIDTNKG
                     NLILELLYDNAPMTVQNFIDLAQGEKEFLLRGEKVKKPFYNGLTFHRVISGFMIQGGC
                     PNGDGTGGPGYTFDDEINAVSLGLDKIKVKDAPSYGRYLQKALFVGMGIRNQQQLDER
                     ITEADANLKKASEMSVVEVLARNGYKYNEVLTSKKALKGSLAMANSGPNTNGSQFFIN
                     QVDTPHLDGLHTVFGQLVNGENVLNQIVADGNSKTIINKVVIIDKRG"
     gene            807..1427
                     /locus_tag="PLF66_23460"
     CDS             807..1427
                     /locus_tag="PLF66_23460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67066.1"
                     /translation="MSEVQNKEEAYIEAMTLIAQLSQNDSVSSVLEKISDIPEDLTHK
                     DAILVELDKNRIFPESFMKILTFLIFILSERSEINKLYEESMERYETINKLTAKGRPT
                     EDEAKIKKTLTDFILKIESFYEAHDKAGDAIIKELNRHMTELNIKHNIDREILHYKIS
                     PRSFNQVQPHLIALLDVYFNYKKNKGILKRLIKISNYIIEDALKKA"
     gene            1460..2437
                     /locus_tag="PLF66_23465"
     CDS             1460..2437
                     /locus_tag="PLF66_23465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67067.1"
                     /translation="MLKLSLVFLLCLLPIYSYPDMDVRGNFTENFFGLQGRVNLKLTS
                     MSYKQKEPIYLDVTVKNYGNEVIRIYPTTEHLEAYQFVITDENDEVLPVRETLKAEIL
                     KSSKIRMNNLVGDQVKEIIIHKNESFTKRFNLDDYYEFTPGKKYYITGYFYPNFTEDK
                     NTFAKSENQAIFQLEKKKEERLYKRHHDSEIAKDGLSPEETIYLFLAAELKKNWNHYF
                     KYIYFPEFILGYTKYANEYTSAEPGYKEIVLDEFKKYLTERRSGNLTYFKITSSEEIN
                     PVMAKVYVHVEREINRIPSKFEYQYVLKKTDDTMKGFWKISGVVVRVKK"
     gene            2434..3459
                     /gene="fliM"
                     /locus_tag="PLF66_23470"
     CDS             2434..3459
                     /gene="fliM"
                     /locus_tag="PLF66_23470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020768525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HMZ67068.1"
                     /translation="MTEILSQDEIDALLNAISSGEVNEEEYSAVGEQKKVKIYDFKRP
                     DKFSKDQIRTLQMMHETFARLATTGLSAQLRALVGVHVASVDQLTYEEFIRSIPNPTT
                     LAVINMDPLKGSAILEMDPSISFTIIDRLFGGKGESAKINRELSDIEMSVMEGIIVRI
                     LGNLREAWSTVIDLRPRLGNIETNPQFAQVVPPNDMVVLITLETKVGDVEGMTNLCIP
                     YITIEPIINKLSAQYWYSSIRRGETDENKSVIQERLDGVTIPLITEVGSVDITMMELM
                     NLQMGDVIKLENTPTKADMVIKVGERNKFKCIPGRVGNRLAVQIGEIIEEIPDELLGS
                     TRSEQEY"
     gene            3848..7345
                     /gene="dnaE"
                     /locus_tag="PLF66_23475"
     CDS             3848..7345
                     /gene="dnaE"
                     /locus_tag="PLF66_23475"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010417067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HMZ67069.1"
                     /translation="MAEDFTHLHLHTTYSMLDGAIRIPDLMKYVKSQGMSSVAITDHG
                     NMFGAIEFYKEAVKNGIKPIIGCEFYVSANRSEERELESIPDGNAYHLILLAKNEVGY
                     KNIIKLASRSYTEGFYKKARIDYELLANNNEGLICLTACLAGEVQRKILEGNDTQALA
                     LANRLNEMFRKEDFYLEIQNHGIKEQETCAKAAYNFYKKTGIPLVVTNDSHFLTKEDQ
                     SAQDILLRIGMQKKIDDEMRFGFNQEFYVKNPAEMARLFPEIPEAVRNTLEIRDKCEL
                     TFKFGNHLLPDFKVPEGYDTDSYLVKLVNDGIKRKYPVVTKEIQDRLDFELNTIRNMH
                     FAGYFLIVQDYIDFARRSGIPVGPGRGSAAGSIVSYALGITNIDPLKYNLLFERFLNP
                     DRKDMPDVDTDFCVEKREKVINYIKEKYGKERVGQIITFGSLAAKAALKDVARVLNIS
                     FAESNEISKAFPPKVDSISEALEISNDLKSLRDKNEVNQKLFYIAEKLEGNYRQPGRH
                     AAGVVISPFPLEEIVPLSTVAEKGKTGRSIVTQYDKDQLESVGLIKMDILGLKNLTTL
                     DYAVHLVEQRHGIKIVPDELPIDDPKTYSLLKKANTLGIFQLESNGITDLVAKSQVNT
                     FEEIVALIALYRPGPMDSGMLQDYLDRKSGKQKVTYPHPSCEPILKETFGVAVYQEQV
                     MSISRVVGGFTMGESDVLRKAMAKKKLDLMADLKVKFVAGAKAQNIDEKLASDLFDLL
                     EKFGGYGFNKSHSVAYALVTYQTAYFKANYPTEYMTALLASDGNDTSAIVKFVNNARE
                     MGIKILNPDVTESEASFSIPADNTIRFGISALKGVGSIAANNIIESRKKAGGFKTLSD
                     FLLNIDTHSVNKRVLEALIQVGAFDSFGYTRKCLFESVDILINYATKEQTRKQEGQGS
                     LFMGGGANDSYSLNLPKNSEEWELDEKLKREKLVSGLFLSGHPLDKYKSKLSTLSSIT
                     IEKIDGVSSGAKVELCGLVTSPEVKYTKKNEEFVNFKLEDFTGDIDCTVFPRTYAKYK
                     EIIKEDQAVFIKGILQKVEIGEAELRGQIIVNDLEILNEDNLVKKLEKSLHIKINPRD
                     FGDNQVVNNLYSILTAFKGNSTVFFHLSSDTNLKKVIKAHSHYSVEPTRELLSRLSEL
                     LGSDSVFYTIGDEILKYSA"
     gene            7342..7779
                     /locus_tag="PLF66_23480"
     CDS             7342..7779
                     /locus_tag="PLF66_23480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002972200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67070.1"
                     /translation="MMLQNLLNLISFVTAIRILVFLNSIVLIFHLFVLFQIIPYTIVW
                     AGKLNSLEEMYAFESVSILVNLILLIVVLIRGNIWKVSTSKRLLDIILWIFVFIFALN
                     TIGNLFAATSLELYLATPLTFFLFYLCLRITSQVDNYIGTNKS"
     gene            7760..8887
                     /locus_tag="PLF66_23485"
     CDS             7760..8887
                     /locus_tag="PLF66_23485"
                     /inference="COORDINATES: protein motif:HMM:NF027716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HMZ67071.1"
                     /translation="MEQTNPKTRFLFLDVFRGITIFSMIIVNNPGNWGSIYAPLKHAR
                     WHGFGGADWIFPFFLFISGFAIPISFSKSLTSGKSRLALFIKILRRSLILFLLGIFLN
                     GFPEFNFSTIRIPGVLQRIALVYLFSASLYLFLNRKILLYLASFLLILHYFLITQIPN
                     PDLGEASLEMGKNLAGWIDLKLMPNHLWVFTKTWDPEGILGTIPAISSALFGIYHGTI
                     FFDKKSFSESEYYLGIFSGLLLISLSFLWDIKFPINKNLWTGSFVLLNTGLALLFIHL
                     LYDWIEAKQTKSWYKPFEILGVNAIGAFFLSSLFSKILNIPFLTQVNGKQVGLKVFLY
                     SELFSKNFSSYNASLLWAICYTLIWILFFLILYKRKIFIRL"
     gene            9221..>9864
                     /locus_tag="PLF66_23490"
     CDS             9221..>9864
                     /locus_tag="PLF66_23490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67072.1"
                     /translation="MFLLQDSPGSDDRTNTILSDMQSGKGIYDPNKTRSYRDRNSMSR
                     YDGIFFGIYYDWNTEIGTWSVGTWIWNNLNRYGKYSWQEWFIWYTPPFAKWANPKLSF
                     FLNTSFDNGGASTSSTPLGYTNGQNYISFEVSHKFFPDKLIHIIPKFLTGYVQNNNNV
                     DKRSGISNVLGSIQFLMGGFDFTLNLLYRPEPKLYDTSDPNKNDGRLADPSKQYG"
BASE COUNT         3282 a         1616 c         1868 g         3098 t
ORIGIN      
        1 actcctttca aacgcctaga tacaaaccag ttgaatatac aaaagtaaat gttgttgttc
       61 gcaagcaaga gcaaaccaat gtcgctcttc ccgacaaaaa aaccatttac gcgatcattg
      121 atacaaacaa aggcaattta atcctagaat tgttatacga caacgcccca atgactgtgc
      181 aaaactttat cgatctagcg caaggggaaa aggaattctt actccgtggc gagaaagtga
      241 aaaagccttt ttacaatgga ttgacctttc atcgagtgat ttctggattt atgatccaag
      301 gtggttgccc gaatggggat gggactggtg gtcctggtta tacatttgat gatgaaatca
      361 atgcagtctc gcttggatta gataaaatca aagtaaaaga cgctcctagt tatggtcgct
      421 atctacaaaa agctcttttt gtaggaatgg gaattcgaaa ccaacaacaa ctagacgaaa
      481 gaatcacaga agcagatgcg aacctaaaaa aagcaagcga gatgagtgtt gtagaagtat
      541 tagctagaaa tggttacaaa tacaatgaag ttctaacgag caaaaaagca ctaaagggct
      601 cacttgctat ggcaaattca ggtccgaata caaatggttc ccagtttttt atcaaccaag
      661 tagacactcc tcatctagat ggacttcata ctgtatttgg tcaacttgtg aatggagaaa
      721 atgttctaaa tcaaattgta gcagatggaa attctaagac gataatcaat aaagtagtca
      781 ttattgataa gagaggatag attcgaatga gtgaagttca aaataaagag gaagcttata
      841 ttgaggcaat gaccttaatt gctcaattaa gtcaaaatga ctctgtaagt tctgttctcg
      901 aaaaaatttc tgatattccg gaagatttaa ctcacaaaga tgcgatccta gtggaattgg
      961 acaaaaatcg aatctttcct gaatctttta tgaagattct tactttctta atttttattc
     1021 tgagtgaaag gtcggaaatc aacaaactct acgaagagtc gatggaacga tacgaaacaa
     1081 tcaataagct cacggcaaaa gggagaccta ccgaagacga ggcaaagatt aaaaagactt
     1141 tgaccgattt tattctgaaa attgaatcat tttacgaagc ccatgacaaa gcaggggacg
     1201 cgattattaa agaattaaat cggcatatga cagaactcaa tatcaaacac aacattgata
     1261 gagagattct acattataaa atttccccca gatctttcaa tcaagtgcag cctcatctga
     1321 ttgctctctt agatgtttat ttcaattata agaaaaataa gggaattttg aagcgattga
     1381 tcaaaatatc gaactatatt atcgaagatg cattaaaaaa agcatagttc tttttgattt
     1441 tagccgattc taaaactgta tgctaaagct atctcttgtg ttcttactat gcctattacc
     1501 tatttattcc tatccagata tggatgtgcg tgggaatttt acagagaact tttttggtct
     1561 tcaaggtaga gtcaatttaa agctcacatc tatgagctac aaacaaaaag aacctatcta
     1621 cttggatgta acagtcaaga attatggaaa tgaagtcatt cgcatttatc caactacaga
     1681 acatttagaa gcataccaat ttgtaattac agatgaaaac gatgaagtcc ttccggttcg
     1741 agaaactcta aaggcagaaa tcttaaaaag ttccaagatt cgcatgaaca acttggttgg
     1801 ggatcaagta aaagaaatta ttattcataa aaatgaatct tttacaaaac gattcaatct
     1861 agatgattat tatgaattta ctcccggcaa gaaatactat ataaccggtt atttttaccc
     1921 aaacttcaca gaagacaaaa acacatttgc caaaagtgag aaccaagcga tcttccaact
     1981 cgaaaagaaa aaagaagaaa gactctataa aagacatcat gatagcgaga tcgcaaaaga
     2041 tgggctgagt ccagaagaaa cgatttatct ttttttggca gcagaattaa aaaagaactg
     2101 gaatcattat tttaaatata tatactttcc tgaatttatt ttaggctata caaagtatgc
     2161 aaatgaatat acttccgcag agcctggata caaagaaatt gtcctcgatg aatttaagaa
     2221 gtatttaact gaaagaaggt ctggaaatct gacctatttc aaaattacat cttccgagga
     2281 aatcaatccg gtgatggcaa aagtatatgt gcatgtagaa agagagatca atcgtattcc
     2341 ttctaagttt gaatatcagt atgttttgaa aaaaacagat gacacaatga agggtttttg
     2401 gaaaatttcc ggtgttgtgg taagggtaaa aaaatgacag agatactttc acaagatgaa
     2461 atagatgcat tattaaatgc gatttctagc ggcgaggtaa acgaagaaga atactcagcc
     2521 gtaggcgaac aaaaaaaagt taagatctac gacttcaaac gtccagataa attctctaaa
     2581 gaccaaatta gaaccttgca gatgatgcat gagacctttg ctcgtcttgc aaccacaggg
     2641 ctttctgctc agcttcgtgc gttagtggga gtgcacgtcg catcggtgga tcagttgact
     2701 tatgaagaat tcattcgttc gatcccaaat ccaactacac ttgctgtaat caatatggat
     2761 ccactcaaag gatctgctat tttagaaatg gatccttcga tttcctttac aatcattgat
     2821 cgtctctttg gtggaaaggg agagtctgca aaaatcaatc gagaactttc tgatatagaa
     2881 atgtctgtaa tggaagggat aatcgttcgt atcctcggaa acctccgaga agcttggtct
     2941 actgtaatcg atcttcgtcc tcgtcttgga aatattgaaa ccaatccaca atttgcgcaa
     3001 gtggtgccac ctaacgacat ggtggtttta attacactcg aaaccaaagt tggggatgta
     3061 gaagggatga caaacctttg tattccttat atcacaattg aacctattat taataaactt
     3121 tctgcccagt attggtattc ttcgattcgt cgtggggaaa cagatgaaaa taaatctgtc
     3181 atccaagagc gtttggatgg agttaccatt ccactcatca cagaagttgg ctctgtagac
     3241 attaccatga tggagcttat gaacctccaa atgggagatg taatcaaatt agaaaacact
     3301 cctaccaaag cagatatggt gattaaagtt ggagagcgaa ataaattcaa atgcattcct
     3361 ggtcgtgtag gcaatcgact tgccgttcag attggagaaa tcattgaaga aattccggat
     3421 gaacttcttg gttctacaag gtcagagcaa gaatactagt ctgaactttt taaaatcgct
     3481 tcctgatcca taagtctttg aaaactgact accaccgatg gagacgatgg gttttataga
     3541 tagttggctt tcttatttta catgattcgt tgatttgatc ccatttttaa ctgctagagg
     3601 cgcaatttaa gtagattgaa tataattcat taagccaaat tttaaacgca tcgtttttat
     3661 cgttctgttc tcggtgccct cggtggtcat ctttcttatg ttttgtttaa gtatgtggtt
     3721 tgcaaagaat tacgaaacta tgtgcaaaaa aattatgtat ctttcccctt ttttctattg
     3781 aaaagaaaac aatttcctct ttttatgtaa attacccttt gggaatctgt tctcattatg
     3841 tcgcattatg gctgaagatt ttacacattt acatttacat acaacttatt ccatgctaga
     3901 tggggcaatt cgtatccctg atctgatgaa gtatgtgaag tcgcagggta tgtcctcggt
     3961 cgctatcaca gatcatggaa acatgtttgg tgcgattgag ttttacaaag aagctgtaaa
     4021 aaatggaatt aagccaatca ttgggtgtga attttatgtt tctgccaaca ggtcagaaga
     4081 aagggaatta gaaagcattc cagatggaaa tgcctatcac ttaattctac tcgcaaaaaa
     4141 tgaagtcggt tataaaaata taatcaaact tgcgagtcgt tcctatacag aaggctttta
     4201 taaaaaagct agaatcgact acgaattatt agcaaataac aacgaaggtt taatctgtct
     4261 tacagcctgt ctcgctggcg aagtacaaag aaaaatttta gagggaaatg atacccaagc
     4321 tctagcactt gccaatcgcc tcaatgagat gtttcgaaaa gaggacttct atttagaaat
     4381 tcaaaaccat ggaattaaag aacaagaaac ttgcgcaaag gcagcgtata atttttataa
     4441 aaagacaggc attccacttg tagtaacgaa cgactctcac ttcttaacaa aagaagacca
     4501 aagcgcacaa gatattttgc ttcgaattgg aatgcaaaag aagattgatg atgaaatgcg
     4561 ttttggattc aaccaagaat tttatgtaaa aaatccagcg gagatggcaa ggctatttcc
     4621 tgaaattccg gaagcagttc gaaacacact tgagattcga gataaatgcg aactaacgtt
     4681 taaatttgga aatcatttac ttcccgattt taaggttcct gaaggatatg atactgactc
     4741 ttatcttgta aaattggtaa atgatggaat caaaagaaaa tatcctgtcg taacaaaaga
     4801 aatccaagat cgacttgatt ttgagctaaa tacaattcga aatatgcact ttgcgggata
     4861 ctttttaatt gtgcaggatt acattgattt tgcaagaaga tcaggaattc ccgttggacc
     4921 tggaagaggg tctgctgcag ggtctatcgt ttcttatgca cttggtatta caaacattga
     4981 tccattaaaa tacaacttac tctttgaaag atttttaaat ccagatagaa aggatatgcc
     5041 tgacgttgat actgatttct gtgtagagaa aagagaaaaa gttatcaatt atataaaaga
     5101 aaaatatggg aaggaaagag tagggcagat tattactttt ggaagcttag ctgctaaggc
     5161 tgccttaaaa gatgtggctc gtgttttgaa tattagtttt gccgaatcaa atgaaatcag
     5221 taaagcattt ccaccaaagg ttgattctat ttcagaggct ttagagattt caaatgatct
     5281 caaaagtctg cgagataaaa atgaagtcaa tcagaagtta ttttatattg cagagaaatt
     5341 agaaggaaac tatcgtcagc caggacgaca tgcggcaggg gtagtaattt ctccttttcc
     5401 actcgaagaa attgttcctc tttccacagt cgcagaaaaa ggaaaaacag gaagatccat
     5461 tgtaactcaa tatgataaag accaattaga aagtgttggt ttgatcaaaa tggatattct
     5521 aggtctaaaa aacttaacta ctttagatta tgctgtgcat ttagtagaac agcgccacgg
     5581 gatcaaaatt gttccagatg aattgccaat tgatgatcca aaaacttact cattattaaa
     5641 aaaggcgaat acgttaggga tattccagtt agagtcgaat ggaattactg accttgttgc
     5701 gaagtcacag gtcaatacat ttgaggagat agtagctctc attgctctct atcgtcctgg
     5761 tcctatggat tctggaatgt tacaggatta cttagatcga aagagcggca aacaaaaagt
     5821 aacctatccg catccgtctt gtgagccgat tctaaaagaa acctttggag tagctgttta
     5881 ccaagaacag gtgatgagta tctcgcgtgt tgtgggtgga tttacaatgg gtgaatccga
     5941 tgtgcttaga aaagcaatgg cgaaaaagaa attggatcta atggcagatc taaaagtaaa
     6001 attcgtagca ggggcgaagg cacagaatat agatgaaaaa ttagcttcag acttattcga
     6061 tttattagag aaattcggtg gttatggatt taataaatcg cactctgttg cctatgcgtt
     6121 agtaacctac caaactgcat acttcaaagc aaactatcca acagaatata tgacggctct
     6181 ccttgcatca gatggaaatg atacgagtgc gattgtgaag tttgtgaata acgcacggga
     6241 gatgggaatc aaaatcctaa atccagatgt aacagaatca gaagcatcgt ttagtattcc
     6301 agcagacaat acaattcgtt ttggaatttc tgccctaaag ggtgttggtt cgattgctgc
     6361 aaataatatc attgaatcta gaaaaaaggc aggtggattc aaaacactga gcgattttct
     6421 tttgaatatt gatacacaca gcgtaaataa gagagtccta gaagcgttaa ttcaagtagg
     6481 tgcttttgat tcttttggat atactaggaa gtgtctattt gagtctgttg atatattaat
     6541 caattatgca accaaagagc agactcgtaa gcaagaaggg cagggaagtt tatttatggg
     6601 aggcggggca aatgatagtt attcgctcaa tcttcctaaa aattcagaag agtgggaatt
     6661 agacgaaaaa ttaaagagag aaaaattagt ttctggttta ttcctatccg gtcatccact
     6721 tgataaatac aaatccaagt tgtccacttt atcttctatt accattgaaa aaattgatgg
     6781 ggtttcctcc ggagcaaagg tagagttatg tggtctagtg actagtccag aagtaaagta
     6841 tacaaagaaa aatgaagaat ttgtcaattt taagttagaa gattttactg gtgacattga
     6901 ttgcacagtt tttcctcgca cctatgcaaa atacaaagag attataaaag aagatcaggc
     6961 agtatttata aaaggaattt tacagaaggt ggaaatagga gaggcggaac ttcgtgggca
     7021 aatcattgta aatgatctag aaattttaaa tgaagacaat ctagttaaga aattagaaaa
     7081 atctcttcat ataaaaatca acccgagaga ttttggagac aatcaagttg taaataatct
     7141 ctactccatt ctcactgcat ttaaaggaaa ttcgaccgta ttctttcatc tatcgagtga
     7201 tacaaatcta aaaaaagtaa ttaaagccca tagtcattat tctgttgagc caactagaga
     7261 acttctttct cgcctttctg aattattagg aagtgatagt gtcttttata cgataggcga
     7321 tgagattcta aaatattcag catgatgctt caaaaccttt tgaatctaat ttcgtttgta
     7381 actgcaattc gcatcttagt ctttttgaat tctatcgttt taatttttca tttattcgta
     7441 ttatttcaaa tcattcctta tacaattgtt tgggcaggta aattaaattc tttagaagag
     7501 atgtatgcat ttgaatctgt ttcaatcctt gtgaatctaa ttttgttaat tgttgttttg
     7561 attcgcggaa atatctggaa ggtttcgact tcaaagcgtt tattggatat tattctttgg
     7621 atttttgttt ttatattcgc acttaataca attggcaatt tatttgctgc gacaagttta
     7681 gaactatatc tcgctacacc acttacattc tttctgtttt atctctgtct acgtattact
     7741 tctcaagttg ataactacat tggaacaaac aaatcctaaa actagatttt tatttttaga
     7801 tgtatttcgt ggaatcacaa ttttttccat gatcattgta aacaacccag ggaattgggg
     7861 aagtatttat gccccactca aacatgctag atggcatgga tttgggggag ccgattggat
     7921 ttttcctttt tttttattta tttctggatt tgcaatcccc atctcttttt cgaaatcact
     7981 tactagcggt aaatcaagat tagccttatt tataaagatt ttaaggagaa gtttaattct
     8041 ttttttactt ggtatttttc taaatggatt tcctgagttt aatttttcta ctatacgaat
     8101 tcctggagtc ttgcaaagaa tcgcactcgt gtatttattt tctgctagtc tttatttatt
     8161 tctaaataga aaaattcttt tatatctagc atcttttctg cttatcctcc attatttcct
     8221 aatcactcaa ataccaaatc cggatttagg agaggcaagt cttgagatgg gaaagaattt
     8281 ggctggttgg attgatttga aacttatgcc aaatcatctc tgggtattta caaagacttg
     8341 ggatccagaa ggaattttag gaacaattcc agcaatatct tctgctttat ttggaattta
     8401 tcatggaact attttctttg ataagaaaag tttttcagag tctgagtatt atcttggaat
     8461 attctcgggg ttacttttga ttagcctctc ttttctttgg gatataaaat ttccgatcaa
     8521 taaaaatctt tggactggat cctttgtatt attaaacaca ggacttgcac ttcttttcat
     8581 tcatttgctc tatgattgga ttgaagccaa gcaaacaaag agttggtata aaccatttga
     8641 aatactaggt gttaatgcta taggggcatt ctttttatcg agtctgtttt ctaaaattct
     8701 gaatatacca tttctaaccc aagtaaatgg aaaacaggtt ggactcaaag tatttctata
     8761 cagtgaacta ttttccaaaa acttttcttc gtataacgcc tctttgcttt gggcaatttg
     8821 ttatactttg atttggattc ttttctttct cattttatat aaacgtaaaa tatttattcg
     8881 actctaatcg gtttttactt gtcattttcg gacaagagag agagctttat cgaatgtttc
     8941 ctttcattct atttatttta ctcttttgta attattcttt attctcgcag gaggaagaag
     9001 agaagccgat gtggatgtct gttcgtgtaa catccgatca taattttcgc ggtataatta
     9061 tggagcgtta gctagccaga gaaataacac agcttataca aattatacat atgctcctgc
     9121 agttcagcct cagtttattt actctgcacc gttaaaagga ttgaaattgc tattttgggg
     9181 aaactttttt ttaaacaact tgaaggatag agataacgat atgtttctat tgcaagatag
     9241 tcccggaagt gatgatagaa caaatacgat tctctctgat atgcaatcag gtaaaggaat
     9301 atatgatcca aataaaacaa gatcttatag agatagaaat tcaatgagcc gctatgatgg
     9361 aatctttttt ggaatttatt atgattggaa tacagagatt ggaacttggt cagttggaac
     9421 ttggatatgg aataatttaa accgttatgg gaaatactcc tggcaagaat ggtttatttg
     9481 gtatactcct ccatttgcaa aatgggcaaa tccaaaactt agcttctttt taaatacttc
     9541 ttttgataat gggggggctt ctacttcttc cactcctctt ggttatacaa atggacagaa
     9601 ttatatttca tttgaggtaa gtcataaatt ttttcctgat aagctaattc atattatccc
     9661 taagttttta acaggctatg ttcagaacaa taataatgta gataagaggt ctggaatatc
     9721 gaatgtatta ggttcgattc aatttttaat gggaggattt gatttcactt tgaatctctt
     9781 atatagacca gaacctaagc tttatgatac atccgatcca aataaaaacg atgggagact
     9841 tgcagaccct tccaaacaat acgg
//
LOCUS       DAOQOS010000170         9749 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_3711_length_9749_cov_5.790489, whole genome shotgun sequence.
ACCESSION   DAOQOS010000170 DAOQOS010000000
VERSION     DAOQOS010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 9749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9749
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3711_length_9749_cov_5.790489"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..404
                     /gene="rhuM"
                     /locus_tag="PLF66_23495"
     CDS             <1..404
                     /gene="rhuM"
                     /locus_tag="PLF66_23495"
                     /inference="COORDINATES: protein motif:HMM:NF024706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RhuM family protein"
                     /protein_id="HMZ67073.1"
                     /translation="NRADAEKEFMGLTNFSGALPTKKDISIAKNYLNKDELGRLNRLV
                     SAFFDLAEMKAMEQTPMKMKDWLAELDKFTSMYGKGTLQNSGTVSNEKAIEKAESEYK
                     KYQVKTISPVEEAYLDTIKNMQKKIEKKAKK"
     gene            401..3310
                     /locus_tag="PLF66_23500"
     CDS             401..3310
                     /locus_tag="PLF66_23500"
                     /inference="COORDINATES: protein motif:HMM:NF012027.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III restriction-modification system
                     endonuclease"
                     /protein_id="HMZ67074.1"
                     /translation="MKGFNFEKDLAHQKQAVENTISVFDNVTLNFPSGINKNQFNPFF
                     DFANDKTFSKNIRRIQEENAINEKPDPSTPILDIMMETGTGKTYTYTKTIFELNRQFG
                     IFKFIIVVPTLSIKAGTIDFLKSESCREHFKEQYGKILQLHIVESQKGPKAKKSYLPP
                     SVMSFANAGNFEKGNIQVMVINAGMINSETMKKSFDRKLFDEYSTPFSALSAIGTFMI
                     IDEPHKFGESNTTWTNIQKMNPQCILRYGATFPDKETKQKNGNKAKDYKNLIYQLTAV
                     DAFHRNLVKGVIGHITEFDAGKNAIVKLIDSDGTEASFELTENGKKKTIKLSQKENFK
                     KVHPEMTDLTIENLNKTKVVLSNGLELSKGDKLNPYSYSEKLQETVIQKAIQNHFALE
                     KQFLTRDVKIKPLTLFFIDNIEEYRNKEGYIRKTIEEYARSEIKNILKTENNSFYKSY
                     LEKSLEDLSLTHAGYFSKDNTEKDEAIEKEINEILHDKQAMLDLENPRRFIFSKWTLR
                     EGWDNPNVFQICKLRSSGSDISKLQEVGRGLRLPVNQYGNRVKDEEFFLNYFVDFTES
                     DFVEKLVCEINEKSGTISIEETPDKLTDVMIQRICELYETKESELLEQLDANNAITRA
                     NSFKEGGFAFIKENYPKIFGGINSDKVRKATHKDKKLTIRKEKYSELKYLWEKLNEKV
                     ILEYKFENEDLFKTLVIDFLKEEKLFNQDHIHEKIAKVEIQENKAIANESHSIENRKL
                     GSISTMKYSIFLEELSRLICINKKTLHEAILESGKDLSPYLNQKTLREIKQNFDHFLM
                     ANAFDKFSIEYKKISNSVHPTKFTDAKGNILTEISPSDVGRQYSREEVAEAYLFDVLY
                     HDSDLEKVNIKEKIREVIVFTKIPKNSIKIPVAGGKSYSPDFAYVLKLGNGKQKLHFI
                     IETKDVRGDEGLREEEKYKIKHAEKFFGDNVKIQFRTQFQNDKIVDLIQEITFSM"
     gene            complement(3919..4281)
                     /locus_tag="PLF66_23505"
     CDS             complement(3919..4281)
                     /locus_tag="PLF66_23505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67075.1"
                     /translation="MIIDNTQIYIAELKGKIAFVNESNISFKEISYSTGSFFSDGDTV
                     TQSAIPRFRLRDIPAKSHIIIEDVDVFEDGRIVYSNAKFIETNGEITEVMYLKKREIL
                     PSDPFPGKLIERELEEIS"
     gene            complement(4313..4597)
                     /locus_tag="PLF66_23510"
     CDS             complement(4313..4597)
                     /locus_tag="PLF66_23510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67076.1"
                     /translation="MRQTIVHFFTIRKVSFLAFLTTLILGNFTTILAEEVLLKNGTIV
                     FGRISKISSDEILLDTAKGNQSIPKKDIVRIRHTPLTAEQKANISFLKNE"
     gene            4800..8129
                     /locus_tag="PLF66_23515"
     CDS             4800..8129
                     /locus_tag="PLF66_23515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67077.1"
                     /translation="MKLSIGNNQVKTVIRFTLYLFLTLASSNCTAWPLLTSMLLSNEN
                     KKSQILIPPPASSSSGGGQSSDNQVGFLITPTSGIVTSESGTESIFTIKLKKKPNSNV
                     TLNVSSTDSSEIIANPSQVVFTPDNWDIAQTITLKGVNDNLQDGNQLVNIDLGNAISQ
                     DSAYNSLSAGTITAENIDDDSIGFTVSATGGHIVNESGTSSNFTVVLNSPPTDTVTIP
                     VSSSDLTEGTVLPNTLTFTTANWNIPQTVTILGQDDLSYDGNVNFTITLGPTSSSDAA
                     YNDLSQPNIAVINEDNDTAGITIINPTITTNESGSSAVFYVVLQSQPTATVSIPLTNN
                     GSSEGTLSTTSLTFTTSSWNIQQAVTVTGLDDFNLDGNITYSIQVGAPVSLDLAYNSV
                     SPQSVSVTNIDNDSPAFDITVISGHTSEDLNTAFFKVKLLTIPSANVNLDFTSSNTSE
                     GILISGSSLVFTPANWNSYQTVTVRGVNDSLGDGDIAYSIIPSAAVSGDVNYNGLTPA
                     SIAVVNDDNDTAGITVSAAANPLITTESAGKATFTVRLRTKPTANVTIAGIISSNTTA
                     GTVSPSSLTFTSTNWSVPQVVTITGVNNDYFNPSGILYTITIPSPTSPDVNYNSLGTN
                     TVNVKNNDNDTQGYTVSKTKNFITAESGKSDSFTIRLNTQPVGGDVIIPVTSSNPLEV
                     ITSPSTLTFTAANWNTPQTVTMTGQVDGVTDGSKLITVALGTPTLASGNKDYYPDSTG
                     SDYSSIKLPDYNSALANNGMITINNCDIDAKIALCLPPASERITSESGTSFQYYILLS
                     QAPTNTVTFTATVSDANEATVSTTNITKTAANWDTVQLITVTGKNDSTLEAIGQQLDG
                     NKLYNIVHSVASSTDPFYNGFDVADITNITNTDPDIPDIIMTPANSSSNRIILLNNNS
                     TTVTVRLNAQPTANVTFNLSGGGFTFSPSTLTFTSTDWNVNQSITVSYSGAIGSRQAV
                     TSNFASLDTKFNGKAVSDIFFTIVQPGFTIGTISGNTDETGNTRTFTVRLNSPPTANV
                     SIDVISTNTNEVTIQSAATLTFTPANWNTNQTVTVKGVDEMDLDGDQSVLIQLQPATS
                     ADTSYNGLDPADVTVINLDND"
     gene            complement(8338..8625)
                     /locus_tag="PLF66_23520"
     CDS             complement(8338..8625)
                     /locus_tag="PLF66_23520"
                     /inference="COORDINATES: protein motif:HMM:NF021563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2288 domain-containing protein"
                     /protein_id="HMZ67078.1"
                     /translation="MDMYRQTIREGIAEIDWKELEYFYARGQVIYISMDLDLEETAYQ
                     LVQNNTKFVEASMQSGLIGKVTEELAKTWFENQERLLSVVVQPWVLVQRLK"
     gene            8754..>9749
                     /locus_tag="PLF66_23525"
     CDS             8754..>9749
                     /locus_tag="PLF66_23525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67079.1"
                     /translation="MIFDSIVLNYPSLSMITNAILFLIFFFGILWIPEKTLPTKIMAW
                     LKLTNSMVAIGYVLTQSFLYTHPTVRAYSLSAIIFNQVFLIQILYYFPNRIWTKFSKV
                     LLFFQILLGLPLISWIFYLSFHSNYHFDFHGHTIELEIGKALKLFGLYLILFISIGLI
                     SSIVKIFKDKAESRKANLLLFVYIFVGIFLNGLNYSLHKAALFDRGTFMVLFCITSSI
                     GFFFLLVAFFNNTKDRTSFLYKLVGSGFLSFYLIYNVMNFFGSRSHDLSYDTLQLKET
                     ELISLTGQSRNSDFIEEYSLDSDSLNSVYRKIEFDPTFLEIARRSFLRASITNLDS"
BASE COUNT         3374 a         1743 c         1641 g         2991 t
ORIGIN      
        1 acaatcgagc agatgcagaa aaagagttta tgggactcac gaatttttca ggagcacttc
       61 ctacaaaaaa agatatttcc atagccaaga actatttgaa caaagatgaa ttgggacgac
      121 tcaatcgctt ggtctcggct ttctttgatt tggcagaaat gaaagcaatg gaacaaactc
      181 ccatgaaaat gaaagattgg ttagcagaat tggataagtt tacttctatg tatggaaaag
      241 gaaccttgca aaattcaggt acggtcagca atgaaaaagc catcgaaaaa gcagaatcag
      301 aatataaaaa ataccaagta aaaacaattt cacctgtgga agaagcctat ttggatacta
      361 ttaaaaatat gcaaaagaag attgaaaaga aggcgaagaa atgaaaggtt ttaattttga
      421 aaaagatcta gcacaccaaa agcaggctgt ggaaaacact atttctgttt ttgataatgt
      481 tacactaaat tttccttcag gtatcaataa aaatcagttc aatccctttt ttgatttcgc
      541 aaacgacaaa acattttcga agaatattag aaggattcaa gaagaaaacg cgatcaacga
      601 aaagcctgac ccaagcaccc ccatcctcga cattatgatg gaaacgggaa caggaaagac
      661 atacacttat acaaaaacca tttttgaact caatcgacaa tttgggattt ttaaatttat
      721 catagttgtt cctaccctct ctatcaaagc agggacgatt gatttcttaa agtcagaaag
      781 ttgtcgtgag catttcaagg aacagtatgg aaagattttg caactccata ttgtagagag
      841 ccaaaaggga ccgaaagcaa aaaaaagtta tttacctcct tcggtaatga gctttgcaaa
      901 tgccggaaat tttgagaaag gaaacattca ggttatggtt atcaatgcag ggatgatcaa
      961 ttctgaaacg atgaagaaaa gttttgatag aaaacttttt gatgagtatt cgactccttt
     1021 ctctgctctt tcggctatcg gtacttttat gataatagat gagccgcata aatttggtga
     1081 gtctaataca acttggacaa acatccaaaa aatgaatccg caatgtattt tgcgatacgg
     1141 agcaacattt ccggataaag aaactaagca aaaaaatgga aacaaggcaa aggattacaa
     1201 aaatctaata taccaactca ccgccgtaga tgcatttcac agaaatctgg tcaaaggcgt
     1261 gatcggtcat attacggaat ttgatgcggg aaaaaatgcc attgtcaaac taatagattc
     1321 cgatggaaca gaagcgagtt tcgaacttac agaaaatggt aagaagaaaa cgattaaact
     1381 gtcccaaaaa gagaacttta aaaaagttca tcctgaaatg actgatctca cgattgagaa
     1441 tctaaacaag acaaaggttg tgctgtccaa tggtttggaa ttgagcaaag gggacaaact
     1501 caatccctat tcgtattcgg aaaaactaca ggagacagtg atccaaaaag ccattcaaaa
     1561 tcattttgcc cttgaaaaac aattccttac aagggacgtg aaaatcaaac cacttacttt
     1621 atttttcatt gataatattg aagagtatag aaacaaagaa ggctatatca gaaagaccat
     1681 cgaagagtat gcacgctctg aaatcaaaaa tattctaaaa actgagaata atagttttta
     1741 caaatcgtat ttggaaaaaa gtttagaaga cctatctctc actcacgccg gatatttttc
     1801 taaagacaat acggaaaaag atgaagctat agaaaaagaa atcaatgaaa tcttacacga
     1861 caagcaagct atgcttgatt tagagaatcc cagacgtttt atattttcca aatggacgtt
     1921 acgcgaagga tgggacaatc ctaatgtgtt tcaaatttgc aaactacgaa gtagtgggag
     1981 tgacatttcc aaattgcaag aagtaggaag aggacttcgc ttaccagtga accaatatgg
     2041 aaacagagtc aaagacgaag agttcttttt aaactacttt gtagatttta cagaaagtga
     2101 ttttgtagaa aaattagtat gtgagattaa tgaaaagtcg ggcactattt ctatagaaga
     2161 gactcctgat aaactcaccg atgtaatgat tcaaagaatt tgtgagctgt atgagactaa
     2221 agaaagcgaa ttactggaac aactggacgc gaacaatgcc atcacaagag caaactcctt
     2281 caaagaaggt ggatttgcat ttatcaaaga aaattatccc aaaatttttg gtggtatcaa
     2341 ctcggataag gttcgtaaag caacccacaa agataaaaag cttaccatcc gaaaagaaaa
     2401 atattccgaa ctaaaatatc tttgggaaaa gctaaatgaa aaagtcatct tagaatataa
     2461 atttgagaat gaagatttat tcaagacatt ggtgatagat tttttgaaag aagaaaaact
     2521 tttcaaccaa gaccatatcc atgaaaaaat tgccaaagta gaaatccaag aaaataaagc
     2581 catagccaat gaatctcatt ctattgaaaa tcgaaagcta ggaagtattt ctacaatgaa
     2641 atacagtatt ttcctagaag aactttctag gcttatctgc attaacaaga agactttgca
     2701 tgaagcgatc ttagaatcag gcaaagactt gagtccctat ttgaatcaaa aaacgttacg
     2761 cgaaataaag caaaactttg atcatttcct tatggctaat gcatttgata agtttagcat
     2821 tgaatataag aaaatttcaa atagcgttca cccaacaaaa tttacagatg ctaaaggaaa
     2881 catacttaca gaaatttctc cctccgatgt tggaagacaa tattccagag aagaagtagc
     2941 agaggcttat ctctttgatg ttctctacca cgattctgat ttagaaaaag taaacatcaa
     3001 agagaaaatt cgtgaagtca ttgtatttac aaagattcct aaaaactcca tcaagattcc
     3061 tgtcgccggc ggaaagagct actctcccga ttttgcctat gtactaaaac ttggaaacgg
     3121 gaaacaaaaa cttcacttta ttatagaaac aaaagatgtt agaggagatg agggacttag
     3181 agaggaagaa aaatacaaga taaaacatgc tgaaaaattt tttggagaca atgtgaagat
     3241 tcaatttaga acacaatttc aaaatgataa aatcgtggac ttaattcaag agatcacttt
     3301 tagtatgtag ccaaaaacag aaaatattaa ttacaactta taatcagaat tcagagtttt
     3361 tagccagaaa tgactttttt gtatttatga tttaataggc cggttaagac taagacttcg
     3421 ttaaaaaata acccttaaca atttcctcca tctatttcaa accatctctt tcaaaaagac
     3481 tcgcagtgcc cttcttgttt ttaaaaactt tcgcttgtag gggactattt tagaaagagg
     3541 tggatggaaa tttttaccgt gacgagtcta tctcctgatt ttgagaataa accaatctgt
     3601 agtagtgact gtatcgtcta ttagccatct aattattccg aaaaagaatg tgctttataa
     3661 acgatatcgc gatgggtaat atctctgcac actacatagg cgcgaatata atggtatgta
     3721 gttaggcttt gtttttttat attctttttc gatttaaatc ggttaaagtt tcttaagatt
     3781 caaatcttct tccatttaat gtatttaaaa gaagtcattc aacagaacgt taaatccata
     3841 gttttatcgt tctgttctgg gcggtaattg ttcaaaaagg aatgggattt ttcttttggc
     3901 aattggtata attagaaact aactgatttc ttctaactct ctttcaataa gttttcctgg
     3961 aaatggatcg ctcggcagaa tttctctttt tttgagatac attacttcgg tgatctctcc
     4021 atttgtttca ataaattttg cattggaata aacaattctt ccatcttcaa atacatctac
     4081 atcttcgatg ataatatgac ttttcgcagg tatatctctt aatctaaatc ttggaattgc
     4141 agattgagtc acagtatccc catctgaaaa aaaacttccc gtgctatacg aaatttcttt
     4201 aaacgaaata ttgctttcat ttacaaaagc aatcttacct ttgagttctg ctatataaat
     4261 ttgcgtatta tctataatca tattcttttc ctttctcaat tcattttatt ttttattcgt
     4321 tctttagaaa agaaatattt gccttttgct ctgctgtaag aggagtatgt cggattcgga
     4381 cgatgtcttt tttgggaata ctctgattcc cctttgcagt atcgagtaag atctcatccg
     4441 aactgatctt agatattcgc ccgaatacaa ttgttccgtt cttaagaaga acctcttctg
     4501 caaggatagt tgtaaaattt cccaaaataa gggtggtcaa aaaagctagg aagcttactt
     4561 ttcgaatggt aaaaaaatga acaattgttt gtcgcatagc atatttctag taagagagta
     4621 ggtttgcaag cataataaaa tttcacctat ctagtttaaa gtttctttaa tcaggtttca
     4681 ttttctaaag attcaaagat tcgtattgaa ccgaagtctt tcgatcaatt tttgtcaatt
     4741 tattattgat tttactcata ggcatgtata taagataatt tttttttgtc ttatatacta
     4801 tgaagctttc aataggcaac aaccaagtaa aaactgtaat cagatttacc ctttatttat
     4861 tccttacact cgcatcctct aactgcactg cttggccatt gctcacaagt atgcttcttt
     4921 cgaatgaaaa taaaaaatct caaatcctaa ttcccccacc tgcttcgtca agttcaggtg
     4981 gaggacaatc gagtgacaat caagtcggtt ttttaattac gccaacttct ggaatcgtaa
     5041 ctagcgaatc aggcacagaa tctattttta ctataaaatt aaaaaagaaa ccaaatagca
     5101 atgtgacttt aaatgtatcc tcaacagact cttccgaaat aattgccaat ccatctcaag
     5161 ttgtctttac accggataat tgggatattg cgcaaacgat tactctgaaa ggagtaaatg
     5221 ataatttaca agatggaaat caactcgtaa atattgacct cggcaatgca atcagccaag
     5281 atagcgccta caattcgtta tccgcaggaa caatcacagc tgaaaatata gatgatgatt
     5341 ccatcggttt cactgtatct gcgacaggtg gtcatatagt gaatgaatca ggaactagtt
     5401 caaattttac agtagtatta aattcacctc ccacagatac agttacaatt cctgtatcga
     5461 gtagtgattt aacagaagga acagttttac caaatactct tacttttaca acagctaatt
     5521 ggaatatacc tcaaactgta acgattttag gtcaagatga tttgtcctat gatgggaatg
     5581 ttaattttac tatcacctta ggaccaacaa gtagttcgga tgcggcatac aatgatttgt
     5641 cgcaaccaaa tattgccgta atcaatgaag acaatgacac agcaggaatt acaatcataa
     5701 atcctacgat tacaacaaat gaatctggaa gcagtgcagt tttttatgta gttctccaat
     5761 cacaacctac agcaactgta agcataccgc tcacaaataa tggatcttca gaaggaacgc
     5821 tcagcactac aagcttgacc tttactactt ctagctggaa tattcaacaa gcagtaactg
     5881 tcactggctt agatgatttt aatttagatg gaaatatcac ttactccata caagttggtg
     5941 cacctgttag cttggatctt gcgtataata gtgtttctcc gcaaagcgtt agtgtaacaa
     6001 atattgacaa tgactcgcct gcttttgata ttactgtgat aagcggacat acgagtgaag
     6061 acttaaatac agcatttttt aaagtcaaac tacttacaat tccatctgca aatgtaaatc
     6121 tagattttac ctcctcaaat acttccgagg gaattcttat ctctggctct agcttggttt
     6181 ttacaccagc aaattggaac tcttaccaaa cagtcactgt aagaggagtc aatgatagtt
     6241 taggcgatgg ggatattgcc tattccatta ttccgtctgc tgctgttagc ggggatgtga
     6301 attacaatgg attaactcca gcaagtatag cagtagtgaa tgatgacaat gacactgcag
     6361 gcattactgt gagtgcagcg gctaatccat tgattaccac agaaagtgca ggcaaagcaa
     6421 catttaccgt aagattaaga accaagccta cagcgaatgt tactatcgca ggaataataa
     6481 gttccaatac gacagccggc acagtctcac catctagttt aacgttcaca tctacgaatt
     6541 ggagtgttcc acaagtagta actattactg gagtaaataa tgattatttc aatccaagtg
     6601 ggattttata tacaatcacg attccatccc ctacgagtcc agatgtgaat tacaactctc
     6661 ttggaaccaa tacggtcaat gtaaaaaata atgacaatga cacacaggga tatacagtaa
     6721 gtaaaactaa gaatttcatc acggcagagt ctggtaaatc agatagtttt acgattcgtc
     6781 tcaacacgca accagttggt ggagacgtca tcattcctgt aaccagttcc aatcctcttg
     6841 aggttattac ttctccttct actcttacct ttacagcggc aaattggaat actccacaga
     6901 cagtcactat gactggacaa gtagatggtg ttacggatgg atcaaaacta attacagttg
     6961 cattaggcac accaacgcta gcctcaggaa ataaggacta ttatccagac tctactggta
     7021 gcgattatag ttctataaaa cttcctgact ataattctgc attagcgaat aatggcatga
     7081 ttacgattaa taattgtgat atagacgcaa aaattgctct ctgtttacca cctgcttctg
     7141 aaagaattac aagcgaatca ggaactagct ttcaatacta tattttattg agtcaagcgc
     7201 caacgaatac tgtaacgttt accgctactg tatcggatgc aaatgaagca actgtatcca
     7261 ctacaaacat tacaaaaaca gcggcaaatt gggatactgt gcaactcatt actgttacag
     7321 gaaaaaatga ttcaacacta gaagcaattg gacaacaatt agatggaaat aaactctata
     7381 atatagtcca ctcagttgca tctagcacag atccttttta caatggattc gatgtagccg
     7441 atataacaaa tatcacaaat actgatcctg atattcctga tatcattatg acacctgcaa
     7501 actcttcaag caatcgaatt atcttgttga ataacaattc aaccacagta acagttcgac
     7561 tcaatgcaca accaacggct aatgtcactt tcaatctaag tggtggagga ttcaccttct
     7621 caccctccac gcttacattt acctccaccg actggaatgt aaatcaaagc attaccgtta
     7681 gctacagtgg agccattgga agtaggcaag cggttacttc caattttgcg agtcttgata
     7741 caaaattcaa tggaaaagca gtttcggata ttttctttac aatcgttcag cctggtttta
     7801 caattggaac aatttctgga aatacagatg agactggaaa tacaagaact tttaccgtta
     7861 gactcaattc acctccaaca gcaaatgtat cgatagacgt tatatcaaca aacacaaatg
     7921 aagttaccat tcaatcagcg gctactctaa catttacccc cgctaattgg aacaccaatc
     7981 aaactgtaac tgtaaaagga gtagacgaaa tggatctaga cggagatcaa tctgttctca
     8041 ttcaactgca accggctaca agcgcagata cttcttacaa tggtttagat ccagcggatg
     8101 taactgtaat taatttagac aatgactaaa ttaagaaata atcctgatct agtgtagttc
     8161 tcaatcgaat tgttatacaa tttttctaaa actgaaaatt agaattgatg tagggtatga
     8221 aggatagaat gcgagagaat tagaaaagaa gttgaataga aattattcat atcgttaaaa
     8281 aaatagattg ggaaaattca ttcaacttga aaatgctact ttctagaatc taaaaaatca
     8341 tttcagtcgt tgaactaaaa cccaaggctg aactactacc gaaagaagcc tctcttgatt
     8401 ttcaaaccaa gtttttgcta attcctctgt gacctttccg atgagtccac tttgcataga
     8461 tgcttctacg aactttgtat tattttgaac tagttggtaa gcagtctctt cgagatctaa
     8521 gtccatagaa atataaatca cctgacctct cgcataaaag tattctaatt ctttccaatc
     8581 aatttcagcg ataccttctc gaattgtttg tctgtacata tccatatttt aagaaaagaa
     8641 taaaagcctt atttgtaaag catagattca cactatcttt ttcgattttt tattgattga
     8701 tatactcatt ctaatttcaa ttgtatgaat tcattctctt gaaaggtcat aaaatgattt
     8761 tcgattcaat cgttctaaac tacccatctt tatcgatgat taccaatgct attttatttt
     8821 taatcttctt tttcggaatt ttatggattc ccgaaaaaac tcttccaaca aaaataatgg
     8881 cttggttgaa acttacaaac agtatggttg caattggata tgtattgact caatcttttc
     8941 tttatacgca tccaacagta agagcgtatt ccttatctgc cataatcttc aatcaagtct
     9001 ttttgattca aattttatac tatttcccaa atagaatttg gactaaattc agcaaggtat
     9061 tacttttttt tcaaatactg cttggacttc cgctcattag ttggatattt tatctttcgt
     9121 ttcattctaa ctatcatttt gattttcatg gacacacaat cgaattagaa attggaaaag
     9181 cattaaaact tttcggactc tatttaattc tttttatcag tattggttta attagctcta
     9241 tcgtaaaaat ttttaaagac aaggcagaaa gtagaaaggc aaacttgctt ctatttgtgt
     9301 atatttttgt tgggatcttc ttaaatggat taaactacag tctgcacaaa gctgcgttat
     9361 tcgatagagg aacctttatg gttttatttt gtatcacttc ttccattgga tttttctttt
     9421 tgttagttgc tttttttaat aatacaaaag atcgaactag ttttttatat aagttagttg
     9481 gctctgggtt tctttccttt tacttaatct acaatgtaat gaactttttt ggttctagaa
     9541 gtcatgattt atcttacgat acattgcaat taaaagaaac tgaacttatt tctttaacag
     9601 gacaaagtcg aaattctgat tttatcgaag aatattctct cgactcagat agtttaaatt
     9661 ccgtttatag aaaaatagaa ttcgatccaa cttttttaga gatagctcgt cgctcttttc
     9721 ttagagcatc cataacaaat ctagattcc
//
LOCUS       DAOQOS010000171         9690 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_3748_length_9690_cov_5.530566, whole genome shotgun sequence.
ACCESSION   DAOQOS010000171 DAOQOS010000000
VERSION     DAOQOS010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 9690)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9690)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9690
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3748_length_9690_cov_5.530566"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(161..1249)
                     /locus_tag="PLF66_23530"
     CDS             complement(161..1249)
                     /locus_tag="PLF66_23530"
                     /inference="COORDINATES: protein motif:HMM:NF013595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67080.1"
                     /translation="MKQQKFHLTVLFLAPLLFTFVNCPGTSKNSDDSKTFTLAALALQ
                     SNRTSTGASISTLNVTGFNKPEAIGSFSDGSLYVADTKNNQIKKITNETTVTVLLDTN
                     GIAGSPLISPEGLCVDSSGVVYVNNTDGHNIIRINTNGTASTFAGLYNTSGMTNGAVS
                     TTTAMFNGPEGIFCASNSTFYIADVGNNQIRRIQSNTVSLIAGSTAGTAGFNNANGTT
                     ALFNGPKGVAADSSGNIFVADNTNNVIRKISSTGDVTTLAGSAQGTSGNLDGTGTAAR
                     FNKPYALTIDDSGNIYTTDSSNNSIRKITQAGVVTTVVGNGTAGSADGSGTAATLNDP
                     RGIVYDSTKKIIYVSTAGDNKIRKIINF"
     gene            1378..1536
                     /locus_tag="PLF66_23535"
     CDS             1378..1536
                     /locus_tag="PLF66_23535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67081.1"
                     /translation="MSEYGMRFILFIERVFSERFAYIVQRQLGKSVGRHPEDKMIYLS
                     ESEYNLYH"
     gene            1686..1946
                     /locus_tag="PLF66_23540"
     CDS             1686..1946
                     /locus_tag="PLF66_23540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67082.1"
                     /translation="MGDLINKSIRKQNMKYREIKNIGYLLAFPTIATEDYQKYMDSAL
                     SLLDHNFSSSHICILSSLSHPINFFETKEIILNVKEDLHLPT"
     gene            1962..2246
                     /locus_tag="PLF66_23545"
     CDS             1962..2246
                     /locus_tag="PLF66_23545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67083.1"
                     /translation="MLYYSYHLIEKIALKEDVILNLKIVADLCLSHDQLKSIYPFLEL
                     YWAYEDLLYSEEQYYWEDMNRENSMDVIVDFAKNWLGENLDRFEVEFISD"
     gene            2286..2855
                     /gene="gmhA"
                     /locus_tag="PLF66_23550"
     CDS             2286..2855
                     /gene="gmhA"
                     /locus_tag="PLF66_23550"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020768146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HMZ67084.1"
                     /translation="MKRLKEQLIETYNALNILLHEEETENKVTTATKLIVDTLKENHP
                     FLICGNGGSASDSMHIAGELVGRFLKERKALNVICLSSNPAVITAWANDYSYEDIFAR
                     QVEAYGKKGGVLLGISTSGNSKNVIKAFEMAKSLNVKTIALTGKGGGKLSEVADILID
                     VPSNHTPRIQELHIMLYHYICERIEEILS"
     gene            2923..3462
                     /locus_tag="PLF66_23555"
     CDS             2923..3462
                     /locus_tag="PLF66_23555"
                     /inference="COORDINATES: protein motif:HMM:NF033644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UpxY family transcription antiterminator"
                     /protein_id="HMZ67085.1"
                     /translation="MFSENIQSVSDSNSSQQWYAVYTKPRAEKKLKESLEKKSIENYL
                     PLLAEKKKWSDRYKIISSPLFASYLFVKIDFKQDSTKVLREPSSVHFVYHNGKPATIE
                     EDTIGMIRTFLEEYPDKIKVEQAEKLKKGNILEMKNGPFKGKKVTVQKIKNEYYVVVK
                     LPMLNQTVLMEVKKEDLVL"
     gene            3459..4454
                     /locus_tag="PLF66_23560"
     CDS             3459..4454
                     /locus_tag="PLF66_23560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020766845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HMZ67086.1"
                     /translation="MNPPKVFISTYPFGKYDPTPIRILEEKGFEILKNPYTRKLKPEE
                     VAELAEDVDAIIAGTENLTELIERNKKLQLISRVGIGLDSVPLQLCKDKGIVVAYTPD
                     AVTMAVAELTIGLMIDVSRKVQMADREMRKGGWSRFVGKRLGESVIGIIGAGRVGLNV
                     IRLLSEFYPKEILIQDLKDKSKEVAAILDPKRIKYSFISKEEIYRRSDLVSLHVPLTK
                     HTRKMITKKELELFSTDAYLLNTARGELIEEADLIQALSTRKLAGAAIDVFSKEPYSG
                     ELTQLENVVLTEHMGSCSYDCRLAMEREASEEVIRFYSGEALRNEVPAEEYEYQE"
     gene            4798..5142
                     /locus_tag="PLF66_23565"
     CDS             4798..5142
                     /locus_tag="PLF66_23565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016561419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family EPS-associated transcriptional
                     regulator"
                     /protein_id="HMZ67087.1"
                     /translation="MLSDEIRHKIFKAIEENPEVSQRELASLLGVSLGKTNFCLQALM
                     EKGWIKMQNFKNNKNKLAYAYLLTPSGIEEKARLTVRYLRIKMQEYEMLKKEIENLTK
                     EVEESIELPMTH"
     gene            5154..6116
                     /locus_tag="PLF66_23570"
     CDS             5154..6116
                     /locus_tag="PLF66_23570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002733579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HMZ67088.1"
                     /translation="MNSNSKIYVAGHRGLVGSALMRMLTLEGYTNLIGKSKSELDLTD
                     QIKVKEFFEKEQPEYVFLAAAKVGGIYANNTFPAEFIFSNIQIQNNIIDACYRYKVKK
                     LVFLGSSCIYPKFAKQPMDEDQLLDGKLEPTNEPYAVAKIAGIIMCQSYNRQYGTNFI
                     SVMPTNLYGPGDNYHPQNSHVLPALIRRFHEAKINNAPEVVVWGTGKPLREFLFSDDM
                     ARACIYLMKNYDVSGDPKGGQHVNIGSGIEVSIKELAETVKDVVGYPGKLTFDLTKPD
                     GTPRKLLDVSKLHRMGWKHQVELRDGIQIAYDDFCKTGGKQLES"
     gene            6152..6940
                     /locus_tag="PLF66_23575"
     CDS             6152..6940
                     /locus_tag="PLF66_23575"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMZ67089.1"
                     /translation="MKLIADLKKYREILIDLAKRDVKNKYLGSYLGFFWAFLQPLFSL
                     VILWGVFSFGLKIKTDSKVSFFLWLMTGLIPWNFLSEGIVSSANSILENSFLVKKIVF
                     RISLLPIVKVLSSSLVHCFFIFILFLSFLASGYELDIYCLQVFYYFFCGTTFIFVLSW
                     ISSSVIIFFKDIGQIISLSLQFLYWLTPILWSYTVLPEKFKIFLILNPFFYIVEGYRD
                     ALIHKVWFWERGYESLAFFVLNLIIGLFGFFIFRKLKPHFADVI"
     gene            6945..8240
                     /locus_tag="PLF66_23580"
     CDS             6945..8240
                     /locus_tag="PLF66_23580"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMZ67090.1"
                     /translation="MSETVIEVKNLTKVYKLYDKPIDRLKEVLHPFRKRFHKDFFALN
                     DISFNVYKGETIGIIGKNGSGKSTLLKIITGVLTPTSGEVVVKGRISSLLELGAGFNP
                     ELTGLENVFFYGTINGMPRSFMESNLEKILSFADIGDFIHQPVKTYSSGMFVRLAFAV
                     AIQIDPDILIVDEALAVGDIFFQQKCFKRMEELRDRGTTILFVSHDMGSIRNFCSRVI
                     LLKNSVVELIDEPTKVIYEYYRVDTNLIKTEILPSEEKKNSDTNVDSNITTKEENKSL
                     FKGIVQNENIQFVSFQILEKEEYKTNFQIGQTATFVLNFFAKKDISEIYCSIILKNKM
                     DIVVFSGGSYNKQLPLNAKNDQELIFKMDIDLNLEAGQYTVEVSIGSPLKNTFNKGES
                     LIELPKFAPLIIYFDYEKYIPNFYGICGLNYSIEQFVKI"
     gene            8237..>9690
                     /locus_tag="PLF66_23585"
     CDS             8237..>9690
                     /locus_tag="PLF66_23585"
                     /inference="COORDINATES: protein motif:HMM:NF014589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase N-terminal
                     domain-containing protein"
                     /protein_id="HMZ67091.1"
                     /translation="MKILGLSFDYHDSAAALILDGEIVAAVSEERFTRKKNDASFPQK
                     SIDFCLQQGNLNPKDLDFVVFYEDSFLKFNRIVKKSIFDSEKRKKYLWPVINDWLIKG
                     KFSTKDRIKDYLKISSDKIHSIKHHQSHAGSAYYCSDFQEASIITLDGVGEYQTMTIS
                     HGKGNSIQLLEECNLPNSIGLFYSAITAYLGFAVNEDEYKVMGMAGYGKDTYYKQIRD
                     LFTLSDNGTFKIDQSYFEFLNPTELPFNQKFEELLGKRRNPSSEFFLKKDKKYDEEAL
                     LNNKRFADIAASAQKVTEEVIEHIIVSAIKKTNSKNVCLSGGVALNSLANGKIKKKYD
                     LNMFVQPASGDAGSSIGAALYFYNSIQKADSKRVPMSHAYLGPSYTNEEVSIAIQKSL
                     TKNYRFYDTDEDLFKSLIELFLSGKVVGWLNGRSEFGPRALGARSILANPIYPDMQNI
                     VNRSIKFREAFRPFAPAVLEEEAERFFNLPRINN"
BASE COUNT         3271 a         1524 c         1860 g         3035 t
ORIGIN      
        1 tgaaaaataa aaaagaattt tgcttacgca tatataccta aatttaaaaa tatgaaatcc
       61 tattgtcaaa ttctagaaga tgatttttaa gtcatgctga aagtttgaga atggttttta
      121 tttcctttca gcatgacaat gatattaatc agtatttttt ttagaaattt attatcttcc
      181 tgattttgtt atctcctgct gtggatacat agataatttt cttagtgcta tcataaacga
      241 ttcctcgtgg atcatttaaa gtagccgcag ttcctgaccc atcagcactt cctgctgttc
      301 catttcccac tacagtcgtg actacacccg cttgtgtaat ttttcggatg gaattattac
      361 ttgaatctgt tgtatagata tttccactat cgtctatagt tagtgcatag ggtttgttaa
      421 atcttgcagc agttccagtt ccatctaaat ttccagaagt tccttgtgca gaacctgcca
      481 gagttgtgac atcaccagtg ctcgaaatct ttcgaataac gttatttgta ttatctgcta
      541 caaagatatt tccagaagag tcagcggcta ctccctttgg tccgttaaat aaggcagtag
      601 ttccattcgc attattaaaa ccagctgttc cggcagtaga accagcaatg agagaaacag
      661 tattagactg gattcttctg atttgattgt ttccgacatc tgcaatgtag aaagtgctat
      721 tggatgcaca aaaaattcct tctggtccat tgaacatagc agtggttgta gagactgctc
      781 cgtttgtcat accgctagtg ttgtaaaggc ctgcaaaagt agaagcagtc ccatttgtat
      841 tgattcgaat gatattgtgt ccatccgtat tgtttacgta aactacacca cttgagtcaa
      901 cacagagacc ttctggactg atcagagggg agcccgcgat tccattcgta tccagaagaa
      961 ctgttactgt agtttcattt gtaatcttct ttatctggtt gtttttggtg tctgctacgt
     1021 aaagggatcc atctgaaaaa gatccgattg cttccggctt gttaaaaccg gttacattta
     1081 aggtggatat actagcaccg gtagaggttc tatttgattg cagagccaag gctgcaaggg
     1141 taaatgtttt actatcgtct gaattcttac tggttcccgg gcagttcacg aatgtgaata
     1201 ggaggggagc taggaataag acggttaaat ggaatttttg ctgtttcatt ttgctttcct
     1261 aaaattgtaa tgattctaaa tatgagaatg attctcaatt ccattatttc aagtgcaaat
     1321 attctgtcaa ttttatttat acttttacaa atcaagagag gcattttaga gtaggatttg
     1381 agtgaatatg gtatgcggtt tattttattt atagaaagag ttttctcaga acgtttcgcc
     1441 tacattgtgc aaaggcaatt aggcaagtca gttggaagac atccggagga taaaatgatt
     1501 tatctctcag aaagtgaata taatttatat cactaaagat gccaaaagga tttaatgaac
     1561 tcgccttacg caaaattaat aaattgagcc acaaaagatg atttttaaaa tattagagaa
     1621 tgctaataaa ctaataaaac taagagcctg ccttatgttt cgacccttag ggagaaacat
     1681 tgagtttggg tgatttaatt aataaatcta ttagaaagca gaatatgaaa tacagagaaa
     1741 ttaaaaacat tggatatcta cttgcctttc caacgattgc aacggaggac tatcaaaagt
     1801 atatggattc tgctctttct ttgttggatc ataatttttc atcttctcat atttgtattt
     1861 tatctagcct aagtcatcca atcaacttct ttgaaacaaa agaaatcatt ttaaatgtaa
     1921 aagaagattt gcatcttccc acttaagaga tagaagaggc agtgctttac tattcctatc
     1981 atttgatcga gaaaatagct ctaaaagaag acgttatttt aaatctaaaa attgtagcgg
     2041 atctgtgtct atcgcatgat caattaaaaa gtatttatcc ttttctagag ttatattggg
     2101 catatgagga tctactttat tcagaggagc agtattactg ggaggatatg aatcgagaga
     2161 actcaatgga tgtgattgta gattttgcaa aaaactggct cggggaaaat ttggatcgat
     2221 tcgaagttga atttatttca gattaaattc tttacaaata aaaataacta acaaaaggtt
     2281 cttttatgaa acgattgaaa gaacaattaa tagaaaccta caatgctttg aatattttac
     2341 tacatgagga agaaacagag aataaagtta caacagcgac taagttaatt gtagatactc
     2401 tcaaagagaa tcatcctttt ctaatttgtg gaaatggagg ttccgcttct gattcaatgc
     2461 atatagcagg agaactagtg ggacgtttcc ttaaagagag aaaggcactc aatgttatct
     2521 gtctttcttc gaatccagcc gtgataacag cttgggcaaa tgattattcc tatgaggata
     2581 tttttgcaag acaagtagaa gcctatggga aaaagggggg agtgctttta ggtatatcca
     2641 caagtggaaa ttctaaaaat gtgatcaaag catttgaaat ggcaaaaagt ctcaatgtaa
     2701 aaacaattgc actcacagga aaagggggcg gaaaactttc ggaagtggca gatattttaa
     2761 ttgatgtgcc ttccaatcat acccctagaa tccaagaatt gcatattatg ttgtatcact
     2821 atatttgtga gagaatagaa gaaatacttt cctaacaatt ctaagccgat tcaaaaaata
     2881 tttgaccgat cgaattctat agaagcttgt aaaagaaggc gtatgtttag cgaaaatatc
     2941 caatctgtat cggattctaa ttcatctcag caatggtatg cggtttatac aaaaccacgg
     3001 gcagagaaaa aactaaaaga gtcactcgag aaaaaatcaa tcgaaaacta tctaccactc
     3061 cttgcagaga aaaagaaatg gtcggatcgt tataagatca tttcttctcc cttgtttgct
     3121 tcttatctct ttgtgaaaat tgattttaag caagactcca caaaagtttt aagagaacca
     3181 agttctgttc attttgtcta tcacaatgga aaaccagcaa cgatcgagga agatacaatc
     3241 ggaatgatcc gaacctttct ggaagagtat ccagataaaa tcaaagtaga acaagcagaa
     3301 aaactaaaaa agggaaatat tttagaaatg aaaaacggac cttttaaagg caaaaaagta
     3361 acggtacaaa aaattaaaaa tgaatactat gtagtcgtta agctccctat gctcaaccaa
     3421 actgtattga tggaagtaaa aaaagaagac ttggttttat gaatcctccc aaagttttta
     3481 tttccacata cccatttgga aaatatgatc ccacaccaat tcgaatccta gaagaaaaag
     3541 gttttgaaat tttaaaaaac ccttatacaa gaaaattaaa accagaagaa gtggcagaac
     3601 tcgcagagga tgtagatgca atcattgcag gcactgagaa tctaacagaa ttgatcgagc
     3661 gaaataaaaa actacaactc atctcaagag tagggattgg gcttgattct gttcctttgc
     3721 agctatgtaa agacaaagga atcgttgttg cctatacccc tgatgcagtg actatggcag
     3781 ttgcggaatt gaccattggg cttatgatag acgttagtcg aaaagttcag atggcagaca
     3841 gagaaatgcg aaaagggggt tggtctcgtt ttgtaggaaa gaggttaggc gaatcagtca
     3901 ttggaatcat tggagcaggt agggtgggac tcaatgtgat tcggctcctg tctgaattct
     3961 atcccaaaga aattttaatt caggatctaa aagataaatc aaaagaagta gcagccattc
     4021 tagatcctaa gagaatcaaa tacagtttta tttctaaaga agaaatctat cgaaggtcgg
     4081 atctagtatc tcttcatgtt ccgctgacca agcacacacg aaaaatgatt accaaaaaag
     4141 aattagagct attttctacg gatgcctacc ttctcaatac cgccagaggg gagttgattg
     4201 aagaagcgga tttaatccaa gctctttcga ctcgaaagct tgccggtgct gccattgatg
     4261 tattctctaa agaaccctat tcaggagaat tgactcagct agaaaatgta gttttgacag
     4321 agcatatggg atcttgttcc tacgactgta ggctggctat ggaaagagag gcatcggaag
     4381 aggtaattcg cttttattcc ggagaagcac ttcgaaatga ggttccagcc gaggaatatg
     4441 agtatcaaga atagattaag gaaatactaa cgatacacta tctggctacc aactaggaga
     4501 ccaagaagct accccctcgc taggaactgg cttggcttat gctaggggga agccagttgg
     4561 tatgcgagat agtagccaga tagtgtatcg ttaatacgaa cttagggtat agatcaccca
     4621 aggtaagcag ttagaacaga aaaaccctct aaaataggtc ttaaaaagtc attgtcaaat
     4681 aagagagcct caattttact gttcaagtat tgaacatatt gaaaaatata tgtgactcac
     4741 gcggcgaagc tatattcaga gacttcgtca aagccaacat cccacaatca ggtaaatatg
     4801 ctatcagacg aaatccgtca caaaatcttc aaagcaattg aagagaatcc agaagtcagc
     4861 caaagagagc tcgcaagttt actcggagtc agcttgggca agacaaactt ttgtttgcag
     4921 gctcttatgg agaagggctg gatcaaaatg caaaacttca aaaacaataa aaataaactg
     4981 gcttatgcct atttattgac tccaagtggt attgaagaga aagctcgtct cacagtccga
     5041 tacctgagaa ttaagatgca agaatatgaa atgctgaaga aagaaattga aaatttaaca
     5101 aaggaagtag aagagtcaat cgagttgcca atgactcact agagaggttt gatatgaatt
     5161 cgaattcaaa gatatatgta gctggtcata gaggtttagt cggctctgca cttatgcgta
     5221 tgctgacatt agaagggtat acaaatctga ttggaaaatc aaagtcagaa ttagatttaa
     5281 ccgatcaaat aaaggtcaaa gaatttttcg agaaggaaca gcctgaatat gtatttctag
     5341 ctgctgccaa agtgggagga atctatgcaa ataatacatt cccagctgag tttatatttt
     5401 caaacattca aattcaaaat aatatcattg atgcatgcta tcgctacaaa gtaaaaaaat
     5461 tagtcttctt aggttcctct tgtatttatc ctaaatttgc gaaacagcca atggatgaag
     5521 atcaattact cgatgggaaa ttggaaccta ccaatgaacc ctacgcggtg gcaaagattg
     5581 cagggatcat catgtgtcaa agttacaata gacaatatgg aacaaatttt atttcggtta
     5641 tgcctaccaa tctatatgga ccaggtgaca actaccaccc acagaactcc catgtattgc
     5701 cagctctcat tcgtcgcttt catgaagcaa aaataaacaa tgcaccagag gttgttgttt
     5761 ggggaacagg gaaacctctc agagagtttt tgttttcgga cgatatggct agagcttgta
     5821 tctatcttat gaaaaactat gatgtttccg gagatccaaa agggggacag catgtgaata
     5881 ttggctcagg gattgaagtg agtatcaaag aactagcgga gacagttaaa gatgtagttg
     5941 gctatccagg aaaattgacg tttgatttaa ccaaacccga cggaactcct agaaaattat
     6001 tggatgtatc taaactacat cgtatgggtt ggaaacacca agtggagctt cgagatggaa
     6061 ttcagattgc ctatgatgat ttttgtaaaa caggcgggaa acaattagaa tcctaatttt
     6121 ttgagagaga atagaatcac aatatctaaa aatgaagttg attgcggatt taaaaaaata
     6181 tagagaaatt ctaatcgatc ttgctaagag agatgtaaaa aataaatacc ttggctctta
     6241 tctgggattc ttttgggctt ttctgcaacc tttattttct ttagttattt tatggggagt
     6301 tttttccttc ggactaaaga tcaaaacgga ttcgaaagta tctttctttt tatggctcat
     6361 gacgggtttg attccttgga attttttaag tgaaggaatc gttagttctg caaattcaat
     6421 tttagaaaac agttttctag ttaaaaaaat tgtttttaga attagcttgt tgcctatcgt
     6481 aaaagtttta tcttcttcat tggttcattg tttttttatt ttcattcttt tcttatcgtt
     6541 tcttgcttct ggatatgaat tggatatcta ctgtttgcag gtattctatt atttcttttg
     6601 tgggactaca ttcatttttg tattatcttg gattagtagt tctgttatca ttttctttaa
     6661 agatataggg caaatcatct ccttaagttt acaattttta tattggctta ctcctattct
     6721 ttggtcttat actgttcttc ctgaaaaatt taaaatattc ttaattctaa atccattttt
     6781 ctatattgtt gaaggatata gagatgcttt aattcataaa gtttggtttt gggaaagagg
     6841 atatgagtcc ttggcttttt ttgtattaaa tctcatcatt ggactcttcg gattttttat
     6901 ttttcgaaag ttaaaacctc attttgctga tgtaatttaa tcctatgagc gaaacagtca
     6961 ttgaagttaa aaatttaaca aaggtttata aattatatga taaaccaatt gatcgtttaa
     7021 aagaagtatt gcatccattt cgaaaacgat ttcataaaga tttctttgct ctcaatgata
     7081 tcagctttaa tgtctataaa ggcgaaacca ttggtatcat tggcaaaaat ggatctggta
     7141 aatctaccct tctgaaaatc attacagggg tgctcactcc tacttctgga gaagtagttg
     7201 taaaaggcag aatctcctct ttattagaat taggagcggg atttaatcca gaactcactg
     7261 gtttagagaa tgtatttttt tatggaacaa tcaatggaat gcctagatcc ttcatggagt
     7321 cgaatcttga aaaaatactc tcctttgctg atattggaga ttttattcat caaccagtaa
     7381 aaacctattc cagtggaatg tttgttcgat tagcctttgc tgtcgccatc caaatcgatc
     7441 cagatatttt aattgtagac gaagctcttg cggtaggcga tatcttcttt caacaaaaat
     7501 gttttaaacg aatggaagaa ctacgagatc gaggcacaac gattctattt gtttcccatg
     7561 atatgggttc tattcgtaat ttttgttcta gagtaatttt acttaagaat agcgttgttg
     7621 agttaattga tgagccaacg aaagtaattt atgagtatta tagagtagat acaaatctga
     7681 taaaaacaga aatattgcct tcggaagaaa aaaagaatag tgataccaat gtagattcaa
     7741 atattactac gaaggaagaa aacaaatctc tttttaaagg tatcgttcag aatgaaaata
     7801 tacagtttgt atcttttcaa attttggaaa aagaggaata caaaacgaat tttcaaattg
     7861 gtcaaaccgc aacatttgta ttaaattttt ttgcaaagaa agatatctct gaaatatact
     7921 gctcaatcat attaaaaaat aaaatggata ttgttgtatt ttcgggagga tcatataaca
     7981 aacaattgcc tttaaatgca aaaaatgacc aagaattaat ttttaaaatg gatattgatt
     8041 taaacttgga ggctggacaa tacacagtcg aagtttcaat tggaagtcct ttgaaaaata
     8101 cttttaataa aggagaaagt ttgatagaat tacccaaatt tgcgcctcta attatttatt
     8161 ttgattatga gaagtatatc cctaactttt atgggatttg cggtttaaat tactctatag
     8221 aacaatttgt aaagatatga aaatattagg cttatcgttt gattatcatg atagtgcagc
     8281 agcattaatc ctagatggag aaattgttgc ggcagtttct gaagaaagat ttaccagaaa
     8341 gaaaaatgat gcttcttttc cgcaaaagtc tatcgatttt tgtttacagc aaggaaatct
     8401 aaatcccaag gatttagatt ttgtagtttt ttatgaagat agttttctca aattcaatcg
     8461 aattgtaaaa aaatcaattt ttgattctga aaaaagaaaa aaatatcttt ggcctgtgat
     8521 aaatgattgg ttaatcaagg gtaaattttc aacgaaagat cgaattaaag attatctaaa
     8581 aatttcttct gataaaattc attccattaa acatcaccag tcccatgctg gttcagctta
     8641 ttattgttcc gattttcagg aagcttctat catcacacta gatggcgtag gtgagtatca
     8701 aactatgacc atctctcatg gcaaaggaaa ttcaatccag ttattagaag agtgcaattt
     8761 accaaattca attggtttat tttacagtgc cataactgct tatttaggat ttgcagtgaa
     8821 tgaagatgag tataaagtta tgggtatggc gggctatggc aaagatacct attataaaca
     8881 gattcgagat ttgtttacgt taagcgataa tggaacattt aaaattgatc aaagttattt
     8941 tgaattttta aatccaactg aattgccttt taatcaaaaa tttgaagagt tattgggtaa
     9001 aagaagaaat cctagtagtg aatttttctt aaagaaagat aaaaaatacg atgaagaggc
     9061 tcttttaaat aataaacgat ttgcggatat tgcagcgagt gctcaaaaag ttacggaaga
     9121 agtgatcgaa catatcattg tttctgcgat caaaaaaacg aatagtaaga atgtttgttt
     9181 atcaggtggg gttgctttaa attcgttagc aaatggaaag ataaagaaaa aatacgattt
     9241 gaatatgttt gttcaaccgg caagtggaga tgcaggtagt tctattggag cagccttgta
     9301 tttttacaat agcatacaaa aggcagattc taaaagggtt ccaatgagcc acgcttactt
     9361 aggtcctagt tataccaatg aggaagtaag tattgcgatt caaaaaagtt taacaaagaa
     9421 ctatcgtttt tatgatacgg acgaagattt atttaaatct ctgatcgaac tctttctatc
     9481 aggaaaagta gtaggatggt taaatggtag gtctgaattc ggacctagag cacttggcgc
     9541 acgtagcatt cttgccaatc caatataccc tgatatgcaa aatatcgtaa atcgtagcat
     9601 aaagtttcga gaagcattta gaccatttgc acctgctgtg ttagaagaag aagcggaaag
     9661 gttttttaat ctacctcgaa ttaacaatca
//
LOCUS       DAOQOS010000172         9679 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_3753_length_9679_cov_5.914277, whole genome shotgun sequence.
ACCESSION   DAOQOS010000172 DAOQOS010000000
VERSION     DAOQOS010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 9679)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9679)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9679
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3753_length_9679_cov_5.914277"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            94..384
                     /locus_tag="PLF66_23590"
     CDS             94..384
                     /locus_tag="PLF66_23590"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HMZ67092.1"
                     /translation="MNRNEIIKIIQDFKNEYADKYGILKIGIFGSVARGDNTDDSDID
                     IFITMKITNLVLLSRIRQELEEKINTRVDLIQYRDKMNQFLKARIDKEGIYV"
     gene            458..757
                     /locus_tag="PLF66_23595"
     CDS             458..757
                     /locus_tag="PLF66_23595"
                     /inference="COORDINATES: protein motif:HMM:NF014043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HMZ67093.1"
                     /translation="MNFPHEFFDSDENRDKLDSICMMLIAIGESLKNIDKLTNSELLS
                     NYPQVDWRGFKGIRDIISHQYFDVNEEAIFSASKNDIPILLTTLDQIILDLKGSL"
     gene            complement(847..1800)
                     /locus_tag="PLF66_23600"
     CDS             complement(847..1800)
                     /locus_tag="PLF66_23600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010577198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HMZ67094.1"
                     /translation="MIVYFFIDGVGFGENDINQNPFARYANGFFLPLAGKDLPANSKL
                     AEGTYVRTDAAMGIKGLPQSATGQTAIWTGINAPQILQRHVSGFPTFTLRKIIDQYSI
                     LKVLDERGKKAAFLNCYSPVYFETVKKKPKHVSASTMIQQASKIPFRDMEDLRNHNGV
                     FMDITHEFFREFAKSFLPPGDSLMDLRDPFEVGKNVVRIANQYDLVLFEYFLTDKTGH
                     DQNWEMAEKVIHTVERFIDGILDAMDKEKHQLIVTSDHGNLENLTTATHTANPVPTYL
                     YGKYTDSFKDRISALCDIVPLLYEKLGIDIELKWEVAEKQD"
     gene            complement(1797..3038)
                     /gene="glyA"
                     /locus_tag="PLF66_23605"
     CDS             complement(1797..3038)
                     /gene="glyA"
                     /locus_tag="PLF66_23605"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HMZ67095.1"
                     /translation="MYLEKQDPDVFAALKHEDDRQEKSLELIASENFVSQAVLEAYHS
                     TLTNKYAEGYPGKRYYNGCENADRVEELAIERAKKMFNAEYVNVQPHSGAQANMAVFL
                     AALSAGDTFMGMNLAHGGHLTHGSPVNFSGRNYHVVPYGVTKDTNRIDYDEVNKLAKE
                     HKPKLIVVGASAYPRQIDFGKFAEIAKSVGAKLMADIAHIAGLVVTGHHPSPIGICDY
                     VTTTTHKTLRGPRGGLIISSNENEKVLNSRVFPGVQGGPLMHVIAAKAVAFGEALKPE
                     YKTYIQNVVDNARILGEVFVKRGFRVISGGTDNHLVLVDVSVKGITGLDAANELDKVG
                     ITVNKNGIPFDEKPPAVTSGIRLGSPALTTRGLGKTEFEKVGHLICDLLENISDDKKK
                     EQIKGGVAEITKQFPMDRFRL"
     gene            complement(3182..4030)
                     /locus_tag="PLF66_23610"
     CDS             complement(3182..4030)
                     /locus_tag="PLF66_23610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016547328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HMZ67096.1"
                     /translation="MNAKLFYLDQLTKTSPYLNLAIEEAIALNLFQFGYSGAVRFWTN
                     HNTIVLGISDSVSKNIPEDKIREFKNDYPQLRKSYKRKESLYISRRASGGGTVFHDSE
                     FNLNYSLFASTKERKELYPVKDSYKILLGLVTDAIGKQGIAASYAGKSDIAIETDGVL
                     KKISGNAQFRKRDCIVQHGTIILDTKIINLVLENLSHPPEEPDYRKNRSHSDFLTSLP
                     NTFSISRFKIDLYQGFQKFLGNGSLDEIPDRSEQDRNFLAKVFRDAKRLMSEKYANIE
                     FIFSKS"
     gene            complement(4027..4836)
                     /locus_tag="PLF66_23615"
     CDS             complement(4027..4836)
                     /locus_tag="PLF66_23615"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000056208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HMZ67097.1"
                     /translation="MGNSYNYEMRLGPVLTPTVRLLIIINSVVFLIQMLSQLFLSAKI
                     LEGMFALNSNMVNHFYVFQLISYGFLHGDLMHILFNMLNLWFFGCEIESLWGRKNFLI
                     LYFFSIFMGGFLTWIAHNLGFRQGSVLGASGGVLGVMVTYALLWPNREALFLGIFPVK
                     MKYLILILMLLLMFYGGANVAHIAHFGGLLGGAAIFLAKNKYKFDFNFSISRYIQKQK
                     MKRYQEEMYRRQNVKETVDEILDKISKKGMKSLSRKEKQFLKDASTKYYTE"
     gene            4927..5877
                     /locus_tag="PLF66_23620"
     CDS             4927..5877
                     /locus_tag="PLF66_23620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012388015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HMZ67098.1"
                     /translation="MKIKLYGTRGSIASPLTNSAYQEKVLEILGLFAQKQIQSQEGIQ
                     DFFESLPEYLKYTSGGDTTCISVTSDAGKVFMVDCGTGGRNLGDDLMRTEFGKGKGVL
                     ELFFTHTHWDHIQGLPFFKPVYIPGNQINFHSPYNDLEARLIQQQVKEFFPMPFHGTA
                     STKKFRTIQRGEPITYDTVTIDFYPLKHPGGSYAYKFTENGKSFIFATDAEFTGDDMD
                     LIRSMAPFFNGVDMLILDSQYTLDESFAKFDWGHTSYTMAVNCATIWNIKKLVMTHHE
                     PAYGDKKIYEIYEDAKLHKTYLEGSPLEIILAREGLVLEI"
     gene            complement(5938..7083)
                     /locus_tag="PLF66_23625"
     CDS             complement(5938..7083)
                     /locus_tag="PLF66_23625"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HMZ67099.1"
                     /translation="MKRIRLTNWSHLIFKLGFILLLLVSSFSCKKNETTIQSKLSGVV
                     LFAVGDVQSGNKKIKAGDLILESERIQTGKNSTCDIQIRESDSGIVMRIKSESIFELK
                     LAKSEDKKAVNAVLTAGKMMVNVPGKLKAGESFSVITPTSTAGIRGTKFEVEVVGDGS
                     TNYSVTEGRIAAKVYISELEKVPKSIQEKSVTISALNEAIDSEEKIIEAGQKTAIEKA
                     QTDKILNDFGLRDSIVKAQYNSGYTPNQTEVQDSIAALDKSQALKTQEGIQAVKTNAV
                     LKIQTEGTQNLQAKLKEYEELIAIEKKKLETYESVNLAVQERNEKYDEILVKRIEEIT
                     GKSFETLILKNGKKVRGAIFLEGNQYYVITPDGQKTFKEEEVESVEL"
     gene            complement(7106..7558)
                     /locus_tag="PLF66_23630"
     CDS             complement(7106..7558)
                     /locus_tag="PLF66_23630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67100.1"
                     /translation="MLNKLIGTGLLILSILLSGSCSAFVIKHVDSTGKSQTYITDTIF
                     VGTGTFLDCFISPFHWVAGIFYKQPYIHVQGGTLSYPSFGASGCFASSYNHGNGALGL
                     FGIVNLGYFKESPSNAIFFTDTESKERAIIGGNFEATRVTKSINFKTK"
     gene            7777..8625
                     /locus_tag="PLF66_23635"
     CDS             7777..8625
                     /locus_tag="PLF66_23635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67101.1"
                     /translation="MHIQNRLTNNFFFFLILFTFFFACNVLLAESIRLTSGQILEGKI
                     VEESEKILVLQTNKGTFTIKKTDILEMERPGSKETITKAPEDKKLSKLNIALLSFIPG
                     YSPTYNSKERPEIGIPFAMANLFYFYHFLQFQFNSNTLSFQESSEMRNPILALYNYVA
                     LPGYTANQLGIGNPQNSNFFGSYQYLQGAYVYFQIRDIFYLKNKDRVVEGRQMTEEEY
                     LNEKRCYLESYAIASIVNGLVAYFLLSSENNIGALYKTEHNGIRTVFYAVPTPNGASF
                     GAASRF"
     gene            complement(8629..>9679)
                     /locus_tag="PLF66_23640"
     CDS             complement(8629..>9679)
                     /locus_tag="PLF66_23640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67102.1"
                     /translation="ANNLIKQKFLFSSKLFFRFTFIFSFLFTISIHTQNTPQTKEEII
                     AANAKVQVLNWGGKKDASEHIYSLHNYVNLQSLDLGGAYIQNADLKEIQNPKLSYLNL
                     SNTIVTSEGVSTIPVLNQIRTLLLEGTLVNDTLLATISKSNLDRLDIGKTNITDKGIK
                     SLTGARISKLDLTGLKLTNESIASIATIETLQELHLWNVKVTGRNFDTLQKLKSLSVL
                     NLGGSDVGNEGLRYLSKLPNLKVLNLAWTKVSDEGLIDLIQCPKLEILSLNGTKISSK
                     GIGYLSKMKKLKILILENVPLDKNYEDLIAEIPKLKVIYLNKTTANSKSIPTLVKKVP
                     WAAVYYEGVLQFPEE"
BASE COUNT         3040 a         1772 c         1577 g         3290 t
ORIGIN      
        1 aaagattatt tcgatatcag ataattatct gagataaaaa tttttgcttg catagaaaag
       61 aaaacaagtc acattaaaaa caaagatatt ccaatgaata gaaatgaaat tataaaaatt
      121 atccaagatt ttaaaaatga atatgctgat aaatatggaa tcctgaaaat tgggatattt
      181 ggttcagtcg caagagggga taatacggac gatagtgata ttgatatttt tatcactatg
      241 aaaattacta atttagtttt attatccaga atcagacaag aattagaaga gaaaataaat
      301 acgcgtgtag atttaattca gtatagagat aagatgaatc aatttctaaa agcgagaatt
      361 gataaagagg gtatatatgt ttgataaaac ccttgtaatt gaaatacttt tacagatcga
      421 agaggctgct agtagaatcc taaattgaat cagtaaaata aattttcctc atgagttctt
      481 tgattccgat gaaaatcgag ataaattaga ttctatttgc atgatgttaa ttgctatagg
      541 ggaaagtctt aagaatatag ataaattaac taatagtgaa ttgctttcta attaccctca
      601 agttgattgg cgaggattta aaggaattcg agatataata agccatcaat attttgatgt
      661 gaatgaagag gcgattttta gtgcttcaaa aaatgatatt ccaattctgt taacaacatt
      721 agatcaaatc attttagatt taaaaggatc cttataaatc aaagcagaaa tatcttttaa
      781 gtaggaaagt acagtcacat gaccgcgcct tcaatatccg tgttcatcgg tggtaatctt
      841 ctgcggttaa tcttgttttt ccgctacttc ccatttgagc tctatatcaa ttcctaattt
      901 ttcatataaa agtggaacga tatcacagag agcagaaatt cgatctttaa aagagtcggt
      961 atattttcca tagaggtagg tgggaactgg gtttgcggtg tgagtggcag tggtgagatt
     1021 ttctaaattg ccatgatcag aggtaacaat cagttgatgt ttttctttgt ccattgcatc
     1081 taggattcca tcgataaagc gctctacagt gtgaattact ttttctgcca tttcccagtt
     1141 ttgatcgtgg cctgttttat ctgttagaaa atattcaaat aaaacaaggt catattgatt
     1201 tgcaattcgc acaacgttct ttcctacttc aaaaggatct cgtaaatcca taagactatc
     1261 ccccggagga agaaaactct ttgcaaactc gcgaaagaat tcatgcgtta tatccataaa
     1321 gacgccattg tgatttctta aatcttccat gtctctaaat ggaattttag acgcttgttg
     1381 aatcatagta gaagcagaaa catgctttgg tttctttttt acagtttcaa aataaacagg
     1441 gctgtaacaa tttaagaaag cggctttttt tcctctttcg tctagaactt ttaggatgga
     1501 gtattgatct atgatttttc ttagtgtaaa agtaggaaat ccgctcacat gtctttgtag
     1561 aatttgtgga gcgttaattc ccgtccagat ggcagtctgt cccgttgcac tttgaggaag
     1621 accttttatt cccatagcag catccgttcg cacataagtt ccttcggcta acttgctgtt
     1681 agccggaaga tctttgcctg cgagtgggag aaagaatcca tttgcatatc ttgcaaatgg
     1741 attttgattt atatcatttt ctccaaatcc gacaccgtct atgaaaaaat aaacaatcac
     1801 agtctaaagc gatccattgg aaattgtttt gtaatttctg ctactccacc tttgatttgt
     1861 tctttctttt tgtcatctga gatattctca agtagatcac aaatcaaatg ccctactttt
     1921 tcaaattcag tttttcctaa acctcttgta gtaagagctg gagaaccaag acgaattcca
     1981 gaagtaactg ctggcggttt ttcatcaaat ggaattccat ttttatttac agtgattcca
     2041 actttatcta gttcatttgc cgcatcgagt ccagtgattc ctttcacaga aacgtcaact
     2101 aaaactaaat ggttgtctgt tcccccactg atcactctaa aaccacgctt cacaaaaact
     2161 tctccgagta tacgtgcgtt atccacaaca ttttgaatgt atgtcttata ttctggcttt
     2221 aacgcttctc caaatgcaac tgcctttgct gcaatcacat gcataagcgg tcccccctgc
     2281 actcctggga atacacgaga atttaaaact ttttcatttt cattcgaaga aatgataagt
     2341 ccaccccgag gtcctctgag tgttttatga gtagtcgttg taacataatc acaaattccg
     2401 attggagaag gatgatgacc tgttactact agacctgcga tatgtgctat gtcagccatt
     2461 agttttgccc caactgactt tgcgatctct gcaaattttc caaaatcaat ttggcgtggg
     2521 taagcagaag cccctactac gatgagttta ggtttgtgtt ctttcgctaa tttatttact
     2581 tcgtcgtaat caattcgatt tgtatctttt gtaactccat aaggaactac atgataattt
     2641 ctaccactga aatttacagg agaaccatgc gttagatgcc ctccatgtgc tagattcatt
     2701 cccataaaag tatcgccagc actcagtgcc gctaaaaaaa ctgccatgtt tgcttgtgct
     2761 ccagagtgtg gttgcacatt tacatactct gcattgaaca tttttttagc tctctcgatt
     2821 gctaattctt caactctatc tgcattctca cagccattgt aatatctctt gcctggatat
     2881 ccttctgcat atttatttgt gagcgtagaa tggtaagctt ctaatacagc ttgggaaaca
     2941 aaattttcag aagcgattaa ttctaaagat ttctcttgtc tatcatcttc atgctttaaa
     3001 gcagcaaata catctgggtc ttgtttttct aaatacattt tttttcctta tctcggacgg
     3061 tcccgcgtta gcgatgcgta gggcttgttc cctagatgca tcttttcgca tcttgggaat
     3121 gagcgagaat tcgcgaaccc gtagcagcgc ggcgcgatag cgcaacgccc gtaattttat
     3181 ttcaagattt actaaaaata aattctatat ttgcatattt ttccgacata agtctttttg
     3241 catctctaaa aaccttggct agaaagtttc tatcttgctc agacctatca ggaatttcat
     3301 ctaaactacc atttcctaga aatttctgga agccttggta aagatctatt ttaaatctag
     3361 agatggaaaa tgtatttggg agagatgtca aaaaatcaga atgagatcga ttttttcggt
     3421 agtcaggttc ttccggtgga tgcgaaagat tttctaaaac tagatttata atttttgtat
     3481 ccagtataat cgtcccatgt tgcactatac agtctctctt tctaaactgt gcatttccag
     3541 aaattttttt taaaactcca tcggtttcaa ttgcaatatc tgatttgcct gcataacttg
     3601 cggctattcc ttgtttgccg atcgcatccg tcacaagtcc aagtaagatt ttataagaat
     3661 cttttactgg atagagttct tttctttctt tggtggaagc aaatagagaa tagtttaaat
     3721 taaactcact gtcatgaaaa actgtcccac cgcctgatgc tcttcttgaa atatataaac
     3781 tctcttttct tttgtaagat tttctaagtt gcggataatc gttcttaaac tctcgtatct
     3841 tatcttctgg aatatttttt gacacactat ccgaaattcc cagaacaatt gtattgtgat
     3901 ttgtccaaaa tcgaactgcc ccactgtatc caaactgaaa taaattcagc gcaattgctt
     3961 cttcaatcgc tagatttaaa taggggctag tctttgttag ctggtctaag taaaaaagtt
     4021 ttgcgttcac tctgtataat atttagtaga ggcatctttt agaaattgtt tttcttttct
     4081 agaaagagac ttcattccct ttttggaaat tttatctaag atttcatcta cagtttcttt
     4141 tacattttga cggcgataca tttcttcttg gtatcttttc attttctgtt tttgaatata
     4201 acggctgatg gaaaaattaa aatcaaactt gtatttattt ttcgcaagaa aaatcgcagc
     4261 tccaccaagc agccctccaa agtgtgcaat atgcgctaca ttagcaccac catagaacat
     4321 aagtagaagc attaaaatta atattagata tttcatttta acaggaaata ttcctaaaaa
     4381 taaagcttct cgatttggcc aaagaagtgc ataggtaacc ataacaccta aaacaccacc
     4441 ggatgctcct aaaacagagc cttgtctaaa tcctagattg tgagcgatcc aagtgagaaa
     4501 cccacccata aaaatggaaa agaaatagag aattaaaaaa ttctttctgc cccaaagact
     4561 ctcaatttca cacccaaaga accaaagatt gagcatatta aataaaatat gcatcaaatc
     4621 cccgtgcaaa aatccgtaag aaattagctg aaaaacatag aaatggttga ccatatttga
     4681 attcagtgca aacataccct ccaagatttt tgcacttaag aagagttggc ttaacatctg
     4741 gattaaaaaa acgacactgt tgataataat caaaagtctc actgtcggag ttaaaacagg
     4801 tccgagcctc atttcataat tgtaagaatt tcccatagat accataggtg cacaggatat
     4861 ataacttgta aaaactaaat tggtatttat ataggattac tataaaattt aataagaacg
     4921 gctaacgtga aaataaaact ttatggaaca agaggctcaa tcgcctcacc gcttacaaac
     4981 agcgcttacc aagaaaaagt attagagatt cttggattat ttgcacagaa gcagattcaa
     5041 tcacaagaag gcattcaaga tttttttgaa tctttaccag aatacttgaa gtatacgagt
     5101 ggaggagata caacgtgtat ttccgttact tcagatgcag gcaaagtgtt tatggttgat
     5161 tgcgggactg gaggacgaaa tctaggcgat gacctgatgc gaactgagtt cggaaaagga
     5221 aaaggcgttt tagagttatt ttttacccat actcattggg atcatattca aggtcttccc
     5281 tttttcaaac cagtttacat tcctggaaac caaatcaact ttcactctcc ctacaatgat
     5341 ttagaagcaa gactcattca gcagcaagtg aaggaatttt ttccaatgcc gtttcatgga
     5401 actgcttcca cgaaaaagtt ccgaaccatt caaagaggag aaccaatcac ctacgataca
     5461 gttacaattg atttttatcc attgaaacat ccgggtggct cctatgcgta taagtttacg
     5521 gagaatggta agtcttttat ttttgcaaca gatgcagagt tcaccggcga tgatatggat
     5581 ttgattcgat ctatggctcc ttttttcaat ggtgtagata tgttgatttt agattctcag
     5641 tataccttag atgaatcctt tgctaaattt gattggggtc atacctctta cacaatggca
     5701 gtcaactgtg ctaccatttg gaatatcaaa aagctagtca tgactcacca tgagcctgct
     5761 tacggagata agaaaatcta tgagatctat gaagatgcaa aactacataa gacctatcta
     5821 gaaggaagtc ctttagaaat tattctagcg agagaaggat tggttttaga gatttagatt
     5881 taataggtta tcgaatttta aagaaagcga ctaatgaaga ttagccgctt tttttgttta
     5941 tagttctact gattctactt cttcctcttt aaatgttttt tgaccatccg gagtaatcac
     6001 ataatattga ttcccctcta agaagattgc accgcgaact ttttttccat tttttaagat
     6061 gagtgtttcg aaagatttcc ctgtaatctc ttcaattctt tttactaaaa tttcatcgta
     6121 cttttcgttt ctttcttgga cggctagatt tacagactca tacgtttcta attttttctt
     6181 ttcgatcgca atcaactctt catactcttt gagttttgct tgtagattct gagtgccttc
     6241 tgtctggatt tttaatactg catttgtttt tacagcttgg attccctctt gtgtttttag
     6301 agcttggctt ttatctaacg ctgcaatgct atcttgaacc tctgtttgat tcggagtata
     6361 acctgaattg tattgtgctt ttacaatcga atctcttaaa ccaaaatcat ttaaaatttt
     6421 atcagtctgt gctttttcta tcgctgtttt ttgtccagct tcgatgattt tctcttcgga
     6481 gtctatcgct tcatttaaag cagaaatggt aacacttttt tcttgaatgg attttggaac
     6541 tttttctaat tcggaaatat aaacctttgc agcaattctt ccttctgtga cagaataatt
     6601 agtagatcca tctcctacta cttctacttc aaattttgtt cctctaattc ccgctgtaga
     6661 ggttggagta attacagaaa aactttctcc tgcttttagt ttaccaggaa catttaccat
     6721 catctttcct gccgttaata ctgcattaac cgctttttta tcttctgatt tggctaattt
     6781 caattcaaaa atggattcag acttaatacg cattacaata cccgaatcag attcgcggat
     6841 ttgaatatca caggtagaat ttttgcctgt ttgaatcctt tcactttcta gaatcagatc
     6901 gcccgctttt atctttttat ttcctgattg cacatcgcca acagcaaata acactacacc
     6961 ggaaagtttt gattgaatag tcgtttcatt tttcttgcag gaaaaactgg atacaagtaa
     7021 tagcaaaatg aatcctaatt taaagattag atggctccag tttgttaatc gaattcgttt
     7081 cataacttcc tcctttttct taaaattatt ttgttttaaa atttatactt tttgtaactc
     7141 tcgttgcctc aaaatttccg cctatgatag cacgttcttt cgattcagta tccgtaaaaa
     7201 aaattgcatt ggatggactt tctttgaaat agccaaggtt cacaattcca aataacccaa
     7261 gtgctccatt tccatgattg tagctactcg caaaacaacc ggaagctcca aaggatggat
     7321 acgatagtgt tcctccttgc acatgaatat atggttgctt ataaaaaatt cctgcaaccc
     7381 aatggaaggg agaaatgaaa caatctagaa aagttcccgt tcccacaaaa attgtatcag
     7441 tgatataggt ctgactcttt cctgtcgaat caacatgctt aatcacaaaa gcagaacaac
     7501 ttccacttaa aagtatacta agaatcaata accctgttcc aattaactta tttaacatta
     7561 accattgtct ccaataatga aaaatattga tcaatactgt ttaaaaaata ggaaagaaaa
     7621 accgaatctc acctaattca atattgtctt taaatatcgt ctccattgta tcgaatagaa
     7681 acttattgtc actcaaaata cattttccat taaatctaag attagaaatt tatttatata
     7741 ttgacgtttc atatagattt catttattga taagtcatgc atatccaaaa tagattaacc
     7801 aataattttt tcttttttct cattcttttc acttttttct ttgcatgtaa cgttttattg
     7861 gctgagtcca ttcgcctaac aagtggacaa attttagaag gaaaaattgt agaagagtca
     7921 gagaaaattc ttgttctgca aacaaacaaa ggaacattta caataaaaaa aactgatata
     7981 ctcgaaatgg aacgaccagg atccaaagaa acaattacaa aagctcctga agataaaaaa
     8041 ttaagcaaac ttaatattgc cttattgagt tttattcctg gttactcccc tacctacaat
     8101 tcaaaagaaa gacccgaaat cggaattcca tttgcaatgg cgaacttatt ttatttttat
     8161 cattttcttc aatttcagtt taattcaaat actctgagtt ttcaagaatc atcagagatg
     8221 agaaatccga ttctagcgtt atacaattat gttgcccttc ctggatatac agcaaatcaa
     8281 cttggaatag gaaaccctca aaattcaaac ttcttcggat cttaccaata cttacaggga
     8341 gcctatgttt attttcaaat tagagatatc ttctatttaa aaaataaaga cagagttgta
     8401 gaaggaagac aaatgacgga agaagagtat ttaaacgaaa aaaggtgtta tttagaaagt
     8461 tatgcgatag catctatcgt caatggtttg gtagcttatt ttctactctc cagtgaaaac
     8521 aatattggtg ctctttataa aacagaacac aatggaattc gcactgtgtt ctatgctgtc
     8581 ccgactccta atggagcctc ctttggagca gcctctagat tttagaaact attcttctgg
     8641 aaactgcaaa acaccttcat aatagactgc tgcccacgga accttcttta ctagcgtagg
     8701 tatagatttc gaattggcag tggtcttatt taaataaata acttttagct ttggaatttc
     8761 tgcaattagg tcttcataat tcttatcaag aggaacattc tccaagataa ggatttttag
     8821 ctttttcatt tttgataagt atcctatccc cttagaagaa atctttgttc cgttcaaact
     8881 tagaatttcc agtttagggc attgaatcaa atcgattaat ccttcatctg aaactttagt
     8941 ccaagctaaa ttcaaaactt ttaaatttgg gagtttagat aaatatctta aaccttcatt
     9001 tcccacatcg gatccgccca aatttaaaac tgataagctt tttaattttt gtaatgtatc
     9061 aaaatttcta ccagttactt ttacattcca aagatgtaat tcttgtaaag tttcaatggt
     9121 agcgatagat gcaatactct cattggttaa cttcaatcct gttaaatcga gtttagaaat
     9181 tcgcgcacct gttaatgatt taattccttt atcagtaata tttgttttac caatatcaag
     9241 tctatctaaa ttactttttg aaattgtagc aaggagtgta tcattgacta aagttccttc
     9301 caagagtaac gttctgattt gatttaaaac aggaatagta gaaacaccct cactggtaac
     9361 aattgtattc gatagattta ggtaagataa tttgggattt tgaatctctt ttagatctgc
     9421 attttgaata taagctccac ctaaatctaa actttgaaga tttacataat tatgaagaga
     9481 ataaatatgc tcggatgcat ccttctttcc cccccaattt aaaacttgaa ccttggcgtt
     9541 agccgctatg atttcttctt ttgtttgggg agtattctga gtatggatcg aaattgtaaa
     9601 tagaaaactg aaaataaaag taaacctaaa aaacaactta cttgagaata aaaatttctg
     9661 tttaattaaa ttatttgca
//
LOCUS       DAOQOS010000173         9672 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_3757_length_9672_cov_6.199646, whole genome shotgun sequence.
ACCESSION   DAOQOS010000173 DAOQOS010000000
VERSION     DAOQOS010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 9672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9672
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3757_length_9672_cov_6.199646"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..481
                     /locus_tag="PLF66_23645"
     CDS             <1..481
                     /locus_tag="PLF66_23645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67103.1"
                     /translation="LGPNLPQELAEKGESAATGMEVFNQIESAIKEAGFKDYYEFVKV
                     NSKVAWAWNVSQGEIGMQKFQIMKDSGIQRLDESLNDPNVPDDVKAELKKTRQEILDN
                     WAQNKPYADLAMKVVRPLTNEHDLEIIKRNQKILMEAFTGRPIEELQEIDPNLFLKK"
     gene            complement(720..1847)
                     /gene="alr"
                     /locus_tag="PLF66_23650"
     CDS             complement(720..1847)
                     /gene="alr"
                     /locus_tag="PLF66_23650"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008589191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HMZ67104.1"
                     /translation="MLAATRVEISKGAVANNIKSFRSILSPSTRLAVAIKSNAYGHGI
                     ELMAKLAIENKVDVLAVNSLEEAIQLKTLHSPILIMGEIPNIEERLTEINHDNFWIVA
                     SRLKEILLLNKLSNKPRIHLKTDTGMGRLGYGGKDLTKLLTDLKANEVKLSGLMMHFA
                     STEDFTEHSYSMQQLKTFKEYIDQAEKCGYNDIIRHASSSASTMLFKDAHFDMVRIGI
                     SLYGLWPSLETRLSLSLMGKNTFDLSPVLSWKTNVVHLKKVPSGTYIGYGSTFKTNYP
                     TTLAVIPVGYHEGFDRRLSNQGYVLINGERAHVIGRVCMNMTMVDVTHIRDVKIGNEV
                     VLIGKSGKETISADTIANLIGTINYDVVTRIHPSIPRVLVD"
     gene            complement(1851..2135)
                     /locus_tag="PLF66_23655"
     CDS             complement(1851..2135)
                     /locus_tag="PLF66_23655"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HMZ67105.1"
                     /translation="MPIFEYKCETCNKTFEVLQSNSVPYTNCKQAKEDCENKGVLRKM
                     VSSFAFSGFNEGSSSNSFSDYSPSSAGMGCGCHGTSSCPGSSIRDKYGLD"
     gene            2148..2288
                     /locus_tag="PLF66_23660"
     CDS             2148..2288
                     /locus_tag="PLF66_23660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67106.1"
                     /translation="MIPVSNLIANFQGQNMIYQKELFQNRDGTASILDPNGNKIIFSF
                     GK"
     gene            complement(2328..3461)
                     /locus_tag="PLF66_23665"
     CDS             complement(2328..3461)
                     /locus_tag="PLF66_23665"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HMZ67107.1"
                     /translation="MENKVSNLARRIQNHLKNLRSISKEKRELFSADRPVCTEIRRAS
                     LDQNLTDKLIIFLRGTGCSAIDQNGGCTFCGFYTATNHGIKLNRENFLNQLQYAFEVH
                     KKEINQFRIISLYNDGSMLSEHEINLDVTLEMIESLSKFENLKLITIESKLQDITEDK
                     ILKLKNATNKKLEISFGFESSDPMIRRLCINKNFTNDKVVSTIQNLISHDVNPTSLIM
                     VKPPFLTEREAIDDVVKSLVFLENTQVDRIDIELPTVEEFTLTHELWEKGLYQPIMLW
                     SAIEILRQKDLLNLKKQIYISPPNYTVQALAYSSNCPKCDDKISKLFMEYNKKNDFSV
                     FENLKCECKSEWNQRFKLSEIEILPIVDRVEKIMNLLENKPTF"
     gene            complement(3809..4171)
                     /locus_tag="PLF66_23670"
     CDS             complement(3809..4171)
                     /locus_tag="PLF66_23670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67108.1"
                     /translation="MPQEDSIRFENMVAGHLLKWVHRRQDGFGEEWGLRYFKDTTGRE
                     VDFILMLDEIPKLLLETKFSDVDIHPPLKYLKERLPITETWQVYFKGKKDYETPNKIR
                     VSSFSNFWKEWLERYPMF"
     gene            complement(4457..4942)
                     /locus_tag="PLF66_23675"
     CDS             complement(4457..4942)
                     /locus_tag="PLF66_23675"
                     /inference="COORDINATES: protein motif:HMM:NF024571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HMZ67109.1"
                     /translation="MSQARYLEKPISDDLETKMVLIAGPRQVGKTSLSLRIGGKSGYL
                     NWDIPEHRDKILQREFPPIKNILILDEIHKNRSWRELLKSLTDDPIFKRKILVTGSAK
                     LDAYRRGGDSLQGRYFFHRMHPFSGDELNFTNQSDWQQLLTIGGFPEPFLNGEKSFSS
                     R"
     gene            complement(5005..6216)
                     /locus_tag="PLF66_23680"
     CDS             complement(5005..6216)
                     /locus_tag="PLF66_23680"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HMZ67110.1"
                     /translation="MKYILLIFLLATTIYSESIFDKLGGASSKNPKDFYTNASPRAIE
                     LVNKSLKGIDSGNLVDYHTHIVGIEEEKNGTFINPKMRSWLHLKEYFRFKIYLSALKI
                     KSIFNADREAVERLTSLIQNINGHGKYHLLAIDKYYNEDGSVNLNRTEFYVPNEYVFK
                     LCREFPNLFIPTISVHPYRKDAIEELEKWNKEGVRFIKWIPNSQGIDPSNPKLESFYK
                     KLIQLNMILLSHAGEEKAVDAEEDQKFGNPLLLRYPLGLGVKIIIAHCGSLGTNVDLE
                     SPDKKLVDNFDLFMRLMEDPKYQKNLFSDISAVIQFNRDKKVLETLLFRKDLHTRLVH
                     GSDYPLPAVNLVIRLNRLKDFGFLSSDEIEPLKEIYDYNPILFELVVKRTVQWKGNKF
                     SDSIFLKNTNL"
     gene            6362..6610
                     /locus_tag="PLF66_23685"
     CDS             6362..6610
                     /locus_tag="PLF66_23685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67111.1"
                     /translation="MILYTPTELRNNLFTTLDTVKRGETVCIKTREENLYIISEKQLE
                     RLKHSSKTITGSQKIKGRILGNLEEADKLLADYIILPK"
     gene            6776..7162
                     /locus_tag="PLF66_23690"
     CDS             6776..7162
                     /locus_tag="PLF66_23690"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HMZ67112.1"
                     /translation="METDYLLDTHTLIFWLLDEKSLSKKVLKILQKPENNFFVSAMSI
                     LEIQYLVEIGRLEMAIGDLFAYIDSTPNLKIIAFDRPALIESVKLDSHRDPFDRIIVA
                     TSTAYRLKLLTRDRRLLKLFPKIAVW"
     gene            complement(7438..7566)
                     /locus_tag="PLF66_23695"
     CDS             complement(7438..7566)
                     /locus_tag="PLF66_23695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67113.1"
                     /translation="MELSLVETFINLLSWELILGMSLWKYLIAKQLGVELKDLFNF"
     gene            7641..8135
                     /locus_tag="PLF66_23700"
     CDS             7641..8135
                     /locus_tag="PLF66_23700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67114.1"
                     /translation="MQINANKVNGSNFYLTKKDISELEFYLLENKYKIVSDTLKNEQI
                     QLANKLDDASSSKKFIFLPEDESELVNSKTKSGIHIINETTSPVVEYWNPAKWEKNLV
                     MPRGRLYYTTHFYNSENQLVKKSEAFLEFASGLFKWVKKNFKNVKLKGYGYESFLISE
                     ETRS"
     gene            complement(8218..9318)
                     /locus_tag="PLF66_23705"
     CDS             complement(8218..9318)
                     /locus_tag="PLF66_23705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004435606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HMZ67115.1"
                     /translation="MTKIKELKQTAGENWTNWKWHMQNRLNHPVDLKEYIQISVEEEK
                     IFQEAEEFFSFGVTPYYLSLINPNDLNCPIRLQTVPRSGELSKHPLERIDPLGEEAHM
                     PVKGVTHRYPDRALWYLSHNCAVFCRFCTRKRKVGDSSNTPRSEDWEKALTYFQTHSE
                     IKEVILSGGDPLSLSDNQIVFILKELKKISHINHIRIHTRYPVTLPFRITKELCKSLS
                     DFFPLFLVTHFNSAAECTQEASLAIKNLITIGNLTVLNQSVLLRGVNDSKEKLSELNY
                     ALIRMGVKPYYLHQCDEVFGSSHFRVPIEEGISLMKEIRGYHSGITVPVYVADLTGGG
                     GKVPLPTDYLQKTNLDSYIFKNFRGDEFEVSK"
     gene            complement(9400..>9672)
                     /locus_tag="PLF66_23710"
     CDS             complement(9400..>9672)
                     /locus_tag="PLF66_23710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015679674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HMZ67116.1"
                     /translation="EPTYLMAEVHIVMTYKCFNMNPQKLEHLLHTFFGEACLNLDVFD
                     EAGNRHTPREWFTAPLSVIEEVIQLTLSGEILNYRYDSTREAIVKK"
BASE COUNT         3121 a         1683 c         1611 g         3257 t
ORIGIN      
        1 attaggtcca aatcttccac aagagttagc cgaaaaaggg gagagtgctg ctactggtat
       61 ggaagttttt aatcaaatcg aatcagccat caaagaagca ggatttaaag attattatga
      121 gtttgtgaaa gttaattcta aagtagcttg ggcttggaat gtctcccagg gtgagatagg
      181 gatgcaaaaa tttcaaatca tgaaagactc tgggattcaa agattggacg aaagcttgaa
      241 cgaccctaat gtgccggatg acgttaaagc agaactaaag aaaacgagac aagaaatttt
      301 ggacaattgg gcgcaaaata aaccctatgc agatcttgca atgaaagtgg ttcgaccact
      361 tacaaatgag catgatttag aaatcatcaa acgaaaccag aaaattctaa tggaagcttt
      421 tacaggtcga ccaatagaag agttgcaaga gattgatcca aatctttttt taaaaaagta
      481 aaaaaaatgc gcacacaaat cgattttttt gaatcctatt tgaaccacgt cctcacccac
      541 aattttaaca gattaccacc gatgacaccg ataaaagata cgatattcaa aatcgtttct
      601 gtatcgttca gtgatcggcg gtgaaaaaaa ttttatgtcc aactcgcgtt aattaactca
      661 tcttacgcgc aagcccataa aatgaaagtc agagcctgtc ggcaacttgg ctcaatcaat
      721 taatctacta acactcgtgg tatgctcgga tgaattcgag tgactacatc atagttaatg
      781 gtgccgatta gatttgcaat cgtatctgct gagatggttt cctttcctga ttttccaatt
      841 aagactactt catttccaat ctttacatct ctaatatgag ttacatctac cattgtcata
      901 ttcatacaaa ctctaccaat gacatgagct ctctctccat tgattaaaac gtatccttga
      961 ttggaaagtc ttctatcaaa tccttcgtga tatccaacag gaataacggc taacgtcgtt
     1021 ggatagtttg ttttaaaagt agatccatat cctatatagg ttccgcttgg aacttttttt
     1081 aggtgaacga catttgtctt ccaagaaaga actggactta aatcaaatgt atttttgccc
     1141 attaaagaaa gagataatct cgtttctaaa ctaggccata gtccataaag agagattcca
     1201 attcgaacca tgtcaaaatg cgcatcttta aaaagcatgg tggatgcaga ggaggatgca
     1261 tggcggataa tatcattata cccacatttt tctgcttggt caatgtattc cttaaatgtt
     1321 tttaactgtt gcattgaata ggaatgttct gtaaaatctt ccgtgctcgc aaagtgcatc
     1381 atgagtcccg aaagttttac ctcatttgct ttcaaatcgg taaggagttt agttaaatct
     1441 tttcctccgt atccaagtct tcccattccc gtatcagtct ttaaatgaat tcttggtttg
     1501 tttgaaagtt tgtttaataa aagaatttct tttagtctag aagcaacaat ccaaaaatta
     1561 tcgtggttaa tctctgttag cctttcttca atatttggaa tttctcccat tattaaaatt
     1621 ggggagtgga gagtttttaa ttggattgct tcttccagcg aatttacagc aagaacatcg
     1681 actttgtttt ctattgctaa ctttgccatg agttctattc catgaccata agcattcgat
     1741 ttaatcgcga cggctaatct tgtgcttggt gacagaatag aacgaaaaga ttttatatta
     1801 tttgcaacag cacctttaga aatttctaca cgagtagcgg ctaacaagga ttagtccaat
     1861 ccatatttgt cacgaatgga agaacctggg caactcgaag ttccatggca cccacaaccc
     1921 attccagccg aagaaggtga atagtctgaa aaggaatttg aactagaacc ttcattaaat
     1981 cctgagaagg caaaagaaga aaccatcttt cttaaaacac ccttattctc acagtcctct
     2041 tttgcttgct tgcaatttgt ataaggaact gaattggatt gcaatacttc aaatgtttta
     2101 ttgcatgtct cacacttgta ttcaaaaatt ggcatttccc ctcctaaatg attccagtct
     2161 ctaatctaat tgcaaatttt caagggcaaa atatgattta tcaaaaagaa ttatttcaaa
     2221 atagggacgg gactgcatct attttagatc caaatggaaa taaaattatt ttttcattcg
     2281 ggaagtaaaa tctcatttcc gaaatcttct atcggaaatt acaataacta aaaagttggt
     2341 ttattttcta atagattcat tatcttttca actctatcga ctatcggtaa tatttcaatc
     2401 tcactcaatt taaaacgttg attccattct gatttgcatt cacatttgag attttcaaag
     2461 acagagaaat catttttctt attgtattcc ataaacagtt tggaaatttt atcatcacat
     2521 tttggacaat tggaagaata ggctaatgct tgcactgtat aattgggagg gctaatatag
     2581 atttgcttct ttagattgag taaatccttt tgtctaagta tctcgattgc agaccaaagc
     2641 ataatcggtt gatagagtcc tttctcccaa agctcatgtg taagtgtaaa ttcctctaca
     2701 gtaggaagtt ctatatcaat tcggtctact tgtgtatttt ctaaaaaaac taaactctta
     2761 actacgtcgt ctatcgcttc tctttctgta agaaaaggtg gttttaccat gataagtgaa
     2821 gttggattta catcgtgaga aatcaaattt tgaatcgtgc tgactacttt atcatttgtg
     2881 aaatttttat ttatacaaag tcttcgaatc attggatctg aggattcaaa accaaaggaa
     2941 atttctaatt ttttatttgt agcatttttt aattttaaaa ttttatcttc tgttatatct
     3001 tgtagtttag attcaattgt tatcaatttt aaattttcga atttagaaag agattctatc
     3061 atttccaaag ttacatctaa attaatttca tgttctgaaa gcatacttcc gtcattataa
     3121 agactgatga tacgaaattg atttatttct tttttatgga cttcaaaggc atattgtaat
     3181 tgattcaaaa aattttcacg attcaatttg atcccatgat tcgttgcagt ataaaatccg
     3241 caaaatgtgc atcctccatt ttgatcaatg gcagaacaac cagttcctct taaaaagata
     3301 attaatttat cagttaagtt ttgatcaaga ctagctcttc tgatttcagt gcaaacaggt
     3361 ctatcagcag agaacaattc tcttttttcc ttcgaaatgc ttcgaagatt ttttaagtga
     3421 ttttgaattc ttctagctaa gttggagact ttgttttcca agatatgacc tttcagttgg
     3481 tattctcttt caaaggattc ataataatat tgattctgta aactgattta taccaaacca
     3541 ttagattaaa attggaattt aaaaacaaaa aggtgcgttt ggtctaaata cctctttagt
     3601 gttcccagac gtttatatag gcagtatacc aaatgctact gaaataaaat ccttgcctga
     3661 aattatattc gattcgattt catttggggc atatttttaa agtagaaagt agattcagat
     3721 tttataaaat aagtggctat tggttttcca aaaaatgatt tttgaaattt taaaactatt
     3781 tgtttggaat tcatataaaa atgattggtt aaaacatcgg ataacgctct aaccattctt
     3841 tccaaaaatt tgaaaacgaa ctcacccgaa ttttattagg cgtttcgtag tcttttttcc
     3901 ctttgaaata tacttgccag gtttctgtaa ttggaagtct ttctttaaga tattttaaag
     3961 gagggtgaat atcaacatct gaaaatttgg tttctaatag caactttgga atttcatcta
     4021 gcattaaaat aaaatcaact tctcttcctg ttgtatcttt gaaataacgt agcccccatt
     4081 cttctccaaa tccatcttgt cttcgatgca cccattttag taaatgaccg gcgaccatat
     4141 tttcaaaccg aattgaatct tcttgtggca atgaccaatt ccaaagatat agcttctgtt
     4201 ctttctttac ggagcgtaag ggattaacat taagcggagg tatgcgatag gtatagtaaa
     4261 cattatccaa cacatgcaac catctatcaa tcgtttgatg tgatacacct agatcctcct
     4321 ttaagctatt taacgataat ggacttccta caagatgcgg taggcgaatt ccaagtaatt
     4381 ctaatttagc gagatcaatt atattttcta ggctacgaat ttcttcttct actaatctta
     4441 ccctgtataa tctagactat ctactcgaaa aacttttttc tccatttaaa aatggctcag
     4501 gaaaaccgcc tatcgttaat aattgttgcc aatctgattg atttgtgaaa ttcaattcat
     4561 ctccagaaaa aggatgcatt ctatgaaaaa aatatctacc ttgtaaagaa tcgccacctc
     4621 ttcgataggc atctaattta gcagagcctg taactagaat tttcctttta aagatgggat
     4681 catctgttag tgattttaat aattcacgcc agcttcgatt cttatgaatt tcatcgagta
     4741 ttaaaatatt tttaataggg ggaaattctc tctgtaaaat tttatctcta tgctcaggga
     4801 tatcccaatt taagtaacca gattttccgc caattcggag ggaaagagat gtcttcccca
     4861 cctgtctcgg tcctgcaatt aacaccattt tggtttctaa atcatcgcta attggctttt
     4921 ccaagtatct tgcttgactc atgtgactat tttagcccta atcagaaata agtcatgctt
     4981 tttttacgat atatgttaaa aaagtcacaa gttggtattt ttcaaaaaaa tagaatctga
     5041 aaatttattt cctttccatt gcactgttct ttttacaaca agctcgaaga ggataggatt
     5101 atagtcatag atttctttca gtggctctat ttcatctgag gagagaaacc caaaatcttt
     5161 taatcgattc aagcgaatga caagattgac tgcgggtaat ggataatcac ttccatgaac
     5221 aagccttgta tgtaaatctt tcctaaagag tagtgtctct agaacttttt tatcacggtt
     5281 gaattgaatt actgcggaaa tatctgagaa tagatttttt tgatactttg gatcctccat
     5341 tagacgcatg aataaatcaa aattatccac aagcttctta tctgggcttt ctaaatctac
     5401 gttagttcct aagcttccgc aatgagctat aataattttt acccctagac caagaggata
     5461 acgaagcaaa agtgggtttc caaatttctg atcttcttct gcatctactg ctttttcttc
     5521 tcctgcatgg gaaagaagta tcatattgag ttgaatcaat ttcttgtaaa aagattctag
     5581 tttaggattc gagggatcga ttccctgtga attaggaatc cattttataa atcgaactcc
     5641 ttctttgttc catttctcta gttcttctat tgcgtccttc ctataaggat gaaccgatat
     5701 agtaggaata aaaaggtttg gaaattccct acaaagtttg aaaacatatt catttggaac
     5761 atagaactcg gttctattta aatttacaga accatcttca ttgtaatatt tatcaatcgc
     5821 aagtaaatgg tatttcccat gaccattgat attctgaata agacttgtta gccgctcgac
     5881 tgcttctcta tctgcattga aaatactttt tatcttaagt gcacttagat aaatcttaaa
     5941 tctaaaatat tcttttagat gaagccatga acgcattttt ggattaataa aagttccatt
     6001 tttctcttcc tcaattccaa caatatgtgt atgatagtcg actaagtttc cagaatcaat
     6061 tccttttaag cttttgttca cgagttctat tgctcttgga cttgcgttag tgtaaaagtc
     6121 ttttggattt ttagaggatg ctcctcctag cttatcaaaa atactttctg agtaaatggt
     6181 tgtggcgagt aaaaaaatca atagtatata tttcatttga ttctattgga gattcataag
     6241 tttataagtc aataggaatc cttttaaaat caccttacta caagtgcgta aaacttctat
     6301 tattttgata ttctttagta aaatatttga caatagtgaa agtacatagt ttcctgtacg
     6361 tatgattctc tataccccaa ccgaattacg aaataatctc tttactacac tcgatactgt
     6421 gaaaagaggg gagactgtat gtataaaaac tcgggaagag aatttatata tcatctcaga
     6481 gaaacaatta gagcgactta aacattcgtc taagacgatt actggttctc aaaaaatcaa
     6541 aggcagaatt ctaggcaatc tagaggaagc agataaacta ttggctgatt atatcattct
     6601 accgaaatga ttgatcatac acaaatatat gagtttggca aatgaaagat aacgatgatt
     6661 cttgagacaa tagacaatgg aataataaaa tcaatagcct gcggcggcaa gtcctccgct
     6721 aggagcaaat atcaagcgaa cagatggctc acattgttta aggcgagata attaaatgga
     6781 aacagattat ctactggata cacatacttt aatcttttgg cttctcgacg aaaaatctct
     6841 atctaaaaaa gttttaaaaa ttctacaaaa accagaaaat aatttttttg tctctgcaat
     6901 gtctatttta gaaattcaat atcttgttga aattggtcgc ctagaaatgg cgataggcga
     6961 tttatttgcc tacatcgaca gcactcccaa tttaaaaatt atagcctttg acagacccgc
     7021 tctcattgaa agtgttaagc tcgattctca ccgagatcca tttgatagaa tcattgttgc
     7081 aacctctacc gcgtatcgat taaaacttct tacgagagat cgtaggcttt tgaaattatt
     7141 tcctaagatt gcggtgtggt aggggtttaa aagattacca ccgatgaaga cgatgaacac
     7201 cgataggatt tgtattgggt gttaaatgat ggaagtatga gaacggttac tacaaattca
     7261 cttatatacc aaatgacaaa agtaatttcc cagaagaatt ttgatccact agcatttggt
     7321 atataccaaa cccacttttt tatttttaga atgggacttt tcttttgacg tttggtataa
     7381 gatttcgctt gagttagttt taaattatga tgaatcaagt gacaataggc ggtgttttta
     7441 aaaattaaat aaatctttta gttctacgcc aagttgtttt gcaatcaaat atttccacaa
     7501 agacattcct aaaattaact cccaagataa taaatttata aaagtctcaa ctaatgacag
     7561 ttccattaaa agtataaatc gtgagaagtt tggatttgaa attgtagacc aatcagattt
     7621 gcggataact ataactagga atgcaaatca atgccaacaa ggtgaatggt tcaaattttt
     7681 atctaactaa aaaagacatt tccgaattag aattctactt attagaaaat aaatataaaa
     7741 ttgtttctga tactttaaaa aatgagcaaa ttcaattagc aaataaactt gatgatgcat
     7801 cttcttccaa aaaatttatt tttcttccag aagatgaatc tgagttagtc aattcaaaaa
     7861 caaaatcagg cattcatatt attaatgaaa cgacttctcc tgtagttgaa tattggaatc
     7921 ctgctaaatg ggagaaaaat ctagttatgc cacgcggaag actttattat actactcatt
     7981 tctataattc agaaaatcaa ttagtaaaaa agtcagaagc atttttagaa ttcgcatctg
     8041 gattgtttaa atgggtaaag aagaatttta aaaatgtaaa gttaaaagga tacggatacg
     8101 aatccttttt gatctcagaa gaaacaagat cttagatgca gacgacaggc ggaaaacttg
     8161 ccgatatgat tcgggaagaa gagtttcata ataagaaatc cgctatagca gtttagtcta
     8221 cttactcacc tcaaactcat cccctctaaa atttttaaaa atataagaat ctaggtttgt
     8281 tttttgtagg tagtcagtgg gtaatggaac tttgccgcca ccaccggtta gatctgcaac
     8341 atagacagga actgtaatcc cgctatgata gcctcgaatc tctttcatga gagaaattcc
     8401 ttcctcaatc ggaactctaa agtgagaact cccgaagact tcatcacatt ggtggagata
     8461 atacggctta acccccattc gaattaaagc atagtttaat tcggatagtt tttcctttga
     8521 atcatttact cccctaagaa gaacagattg atttaagaca gttaaatttc ctattgtaat
     8581 caaattttta atggcaagac ttgcttcttg tgtgcactca gctgccgaat taaaatgagt
     8641 cactaaaaat aatggaaaaa aatcggaaag acttttacaa agttcctttg taattctgaa
     8701 cgggagtgta acaggatacc tagtatgaat tcgaatgtga ttgatatggg aaatcttttt
     8761 taattctttt agaataaaga caatttgatt gtcagataaa gaaagcggat ctcctccaga
     8821 aaggataacc tctttgattt cagaatgtgt ttgaaaatag gtgagcgctt tttcccagtc
     8881 ttctgagcgg ggggtattgc tactatcgcc cacttttctt tttcttgtgc aaaatctaca
     8941 aaatacagca caattatgag agagatacca aagagctcta tcgggataac gatgcgtaac
     9001 ccctttgact ggcatatgcg cctcttctcc taaaggatct attctttcga gaggatgctt
     9061 acttaattct cctgaccttg gaactgtctg aagtcggatc ggacagttta gatcatttgg
     9121 attgattaaa gataagtagt agggagttac tccaaagcta aaaaattcct ctgcttcctg
     9181 aaaaatcttt tcttcttcaa ccgaaatctg gatatattct tttaaatcta ctggatggtt
     9241 gagtcggttt tgcatatgcc atttccagtt tgtccaattt tcgcctgcag tttgttttaa
     9301 ttctttaatt tttgtcattc tatttagtcc tgctgagtgg gattgtgtaa aagctgatcg
     9361 tagagcgact ttttacatac cctcgacgga tttagaagtt cattttttaa caattgcctc
     9421 ccttgtcgaa tcatatctat agtttagaat ttctccactt aaagtaagct ggattacttc
     9481 ttcaatgacg gaaagtggag cagtaaacca ctctctagga gtatgacgat ttcctgcttc
     9541 atcaaacaca tcgagattaa gacaagcttc tccaaaaaaa gtatggagta aatgttctag
     9601 cttttggggg ttcatgttaa aacatttgta tgtcatcaca atatgaacct cagccattaa
     9661 gtaggtaggt tc
//
LOCUS       DAOQOS010000174         9608 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_3793_length_9608_cov_6.403538, whole genome shotgun sequence.
ACCESSION   DAOQOS010000174 DAOQOS010000000
VERSION     DAOQOS010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 9608)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9608)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9608
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3793_length_9608_cov_6.403538"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            321..2333
                     /locus_tag="PLF66_23715"
     CDS             321..2333
                     /locus_tag="PLF66_23715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010574257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HMZ67117.1"
                     /translation="MNSKFKELLKKVFLSYNFKFFTILFTFGLIIFSFYRFIFFVKYS
                     YRINFSEVNVSTILLAFATGIRFDISTLAILFGALFVLSCIDLLNHKKLYVLLWSFTP
                     ILITIFVLAILIADVLYFEHANKHIGYEAYVFLGKDLILIVGSLLEENKLIFIAGVFT
                     LVAIPFSVYSIYKKYHSYRHLDTPILISSLKALAALILIIIGIRGGLQESPIRTSNAI
                     LSDNNLINNLALNGVYTAITDFKSHSIAKSDRMNLQEAVAIVRNEIKYTGSNFISEKY
                     PLLRKTIPTSKEAKNPNIVLVILENWTGKFINPISSGKVNGKEVAPHFNQLLKEGIFF
                     QRFFASGGRTPNGMMSILTGIPDRPGLTVIRTPQAMGYFSGIASIMKQANYRTIFATG
                     GDLTFDNKHKMMPHWGFEEIYGKKDMDESKRYAIGAWGYDDADVFDVLHSKIKDHKED
                     RPFFATVLTLTTHYPYRAPREEFNIFGKETEDSEFLNVYHYADWAVNNFLEKAKQSSY
                     FENTIFFFVADHTHHRFLDYYEDRNIPFLIYSPSRFKGEIRNDIASQLDVIPTMLGTL
                     NKEVYFSAMGRDLLHSNTNSAYFAYGTVYGWIEDKLFLFRTSDGEGNVNFTVDPPHLQ
                     FDICKKTPDFCLIHNRKAKAFLNTSIELMNQNLIFPADIKDLMNQN"
     gene            2590..3627
                     /locus_tag="PLF66_23720"
     CDS             2590..3627
                     /locus_tag="PLF66_23720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67118.1"
                     /translation="MNKRILSILFCLVFTANVSIFAQEESSSSGSGSGQTQSGLTIKG
                     GIWGLGGKVKSELEDKYFTDDLISNFGFGQFNTGWSTPSKIYPLNPIGIDYYMSGIGP
                     GSLLLGAEMRGIPGVNFTGYTPKYDFVSVNAGGVGIHNAEMKLKNLDLNVGYQLAFGS
                     LLVTPKFQFRNFTTDFKESGTYFGNGFVGIRNTTNNQNTWSGFIGVNLLYKINEASAV
                     FFDFAIDSPILGQLYSSGDFNKTAFFANSGGGASGSTTLYKKTNQEIRGTVIDLGYQH
                     SFGALGLRIGYRSEELRTIYSSYTDVPLAFNSNGNATFLIGETILNSIFYKGSTSTTL
                     QSLYITATYKL"
     gene            complement(3697..4395)
                     /locus_tag="PLF66_23725"
     CDS             complement(3697..4395)
                     /locus_tag="PLF66_23725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67119.1"
                     /translation="MGVKYFNIINFFSKTIFILTIVFLHSCFSYAYQKRFEPYGIDDY
                     QREFQKDKKISIGRNIADPKDNLVKAQFDSILVGEGRQLNITINPNDSKAEVFDTYEF
                     QQGNKAAVLFYDPTSFSKVFNKEDRKVNLKDMFIPESELKEKMYFYRIDYDLIFVELE
                     RSKEDNNFYIKKMVPTNYRFDRDQISRRERSLVINTFKYGLFYAGYILTIPLDAITGI
                     FQVAAYILVGGGVK"
     gene            complement(4396..5181)
                     /locus_tag="PLF66_23730"
     CDS             complement(4396..5181)
                     /locus_tag="PLF66_23730"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HMZ67120.1"
                     /translation="MFIWNVIFLLSSTLLFSQDLPKLEFVKIRGGDFSFDYTYFYQFH
                     SAKSPNPEKKITVRISPFSITKYPITRQYWEKYMGNDFFTPWEKDKLPDCPECPAYGI
                     FYQDAMLFIKKISLKETGREDSYRLPTESEMIYLVTSSEPKYEKILGFGTSMDVSFGK
                     AGYKNLFNLPVNDEIMSGYVWTNDFASNNYLEEFLNAENLTLPIKDPNPKPISSFSYG
                     KILFRFTYDKNENKMNLYRLSFTTSRMLPESEKVFLRFVKKEK"
     gene            5733..8156
                     /locus_tag="PLF66_23735"
     CDS             5733..8156
                     /locus_tag="PLF66_23735"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HMZ67121.1"
                     /translation="MTRKKILLVEDDIIIAMVEIELLKRNNFEVVHTENGEEALDKVL
                     SNETIDLILMDFELGAGMSGAEAAEEILKIKNIPIIFLTSHVEKETIERVRNVNRYGY
                     VIKNSGETFLISSIEMALNLFEAQRSTKESEQRFRNLADSAPVLIWVADENKNWNYFN
                     KQWLIFRGRRMEEEIGRGWLSGIHPNDQEKYFQLCSDAFENKTPYETEYRLQKHDGVY
                     RWVLDRGIPRSGENGHFAGFIGSVIDITDMKEQEDNLRQAAAVFDNTTEGILITDANN
                     FIKAVNPAFTSVTGYSIDDLIGKTPKILNSGKQDKTFYQEMWKSLLNTGKWQGEIWNR
                     RKNGEIYPELLGISAVKDSQNNTISYVAVFSDISKIKAAQDDLDFLAHHDWLTSLPNR
                     LMFQSRLRHAMSRLESGKHLAILLIDLDRFKDVNDSFGHPLGDEVLQLSAIRMHEILS
                     EKDTLARLGGDEFIVLIEDLDILENAGRIAQDLLDAIAKPFYLTNGIEVFLGASIGIS
                     IYPDQGQSVDAIFQHADAALYQAKKEGKGIFRFYNSGITSSIRIRLTIETRLRKAISK
                     GELAVYYQPQVDIRTGKVVAAEALVRWIDPIDGFVSPSHFIPIAEESGLISQIGEWVL
                     REVCNQGNRWLAEGKTLIPIAVNLSPRQFIHGNIVGLVEQVLNETKYPSEFLEFEITE
                     SALMDREEEAIKTLNQLRNMRIRLAIDDFGTGYSSLAHLKRFPVDILKIDKSFVDDIP
                     QNEDDREIAATIIAMGHTLRLKVLAEGVETKEQLDFLNSCGCDLYQGFYKSKPLPADK
                     FIEFIQDKM"
     gene            8227..9558
                     /locus_tag="PLF66_23740"
     CDS             8227..9558
                     /locus_tag="PLF66_23740"
                     /inference="COORDINATES: protein motif:HMM:NF015724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HMZ67122.1"
                     /translation="MNWNLFIRNQNKKAIRQKSIYLSLCFSTLLLTHCMTTGERTIEG
                     NGEIDKYNIRGFTQFTVYTGRDFLGYVNAEGQEKTFDKKVWRGKVKKDTIEQSISISF
                     SKEDLKELKGGFADILSAETGYQSIQSMNLILEKPVQYILEDISIEPEFKTKKELFTR
                     QYIGAVLKVDKIRIEFQNKSGFTLKTEADFQVNQIKFGNNFKHGSTENSVFFAENAFI
                     GYKLFNAPENPESFIVEEDNRIKVVVLPFEVEGTKTKDEKLKSALAEATARSLNMVDN
                     LFVLDRSGYQKILEEQELAKAENYDQKKAVQIGKLMTANTILMGKFSKEGITARITSK
                     MIDVETNALLSNGTIKLDINLKGKNTPMIADEWESKILESYGVKSTKRDSNNISILGT
                     NVGDAYDFYRRGRENYILLDEESLESAIPLFRKAIELDSNYSDAYAILAET"
BASE COUNT         3408 a         1596 c         1611 g         2993 t
ORIGIN      
        1 attaaaatta tattctcttg ataaaagtaa cttagcagcc tgataggtta cgaaattact
       61 aataaacaat acaatctcct ttagaagcta gctgacagat aaaaaaagca ttgggagaaa
      121 aatccacagt ccatcacttc cgctccctca aaggcgaatc ctatttttca ataacgatac
      181 actgtgttta aatctaagtt tttcctacac atgattcgaa cgagatctgg catcccactt
      241 gacatgcccc catttatttg taaggcgagt gatgaaatta aaatcattga cataaatttt
      301 taatctattt cttttaaaga atgaattcaa agttcaaaga attacttaaa aaagttttcc
      361 tttcctataa ttttaaattt ttcacgattc tatttacttt tggtcttatt atatttagtt
      421 tctatcgatt tattttcttt gttaaatatt cttatagaat caatttttct gaagtgaatg
      481 tttctactat tttattagca tttgctacag gcatacgttt tgacatatct acccttgcca
      541 tattatttgg agctttattt gttttatcct gcattgacct actcaatcac aaaaagcttt
      601 atgttttgct ttggtcattt actcctattt taattacaat ttttgtcttg gctattctaa
      661 ttgcggatgt attatacttt gaacatgcaa ataaacatat aggatacgag gcttatgtat
      721 ttctcggaaa agatttaata cttattgtag gttccctatt agaagagaat aaattaattt
      781 ttattgcggg agtttttacg ttagtagcta ttcctttctc tgtctattca atctacaaaa
      841 agtatcacag ctatagacat ttagatactc cgattctgat ttcttctctc aaagctcttg
      901 ctgcacttat tctaattatt attggaattc gagggggact ccaagaaagt ccaattcgaa
      961 ccagtaatgc aattttatct gacaataact taatcaataa tcttgcctta aatggtgttt
     1021 atacagctat cacagatttc aaaagccact ctatcgcaaa aagcgataga atgaatttgc
     1081 aagaagctgt tgccattgtg agaaatgaaa tcaagtatac tggttctaat tttattagcg
     1141 agaaatatcc acttctacga aaaacaattc ctacttcaaa agaagcaaag aatccaaaca
     1201 tagtcttggt gattctggaa aattggactg gcaaatttat aaatcccatc tcaagcggta
     1261 aagtaaatgg caaagaagtt gctccgcatt tcaatcaact attaaaagaa ggaatctttt
     1321 ttcagcgttt ttttgcgtca ggcggaagaa ctcctaatgg tatgatgtct attcttacag
     1381 gaattccgga tagaccgggt ctaaccgtaa tcagaacccc acaggctatg ggatattttt
     1441 ccggtattgc aagtataatg aaacaagcaa attatcgaac catctttgct accggtggag
     1501 atttgacttt tgataataaa cataaaatga tgcctcattg gggctttgaa gaaatctatg
     1561 gaaaaaaaga catggatgaa tctaaaagat atgcgatagg tgcatggggt tatgatgatg
     1621 ccgatgtctt tgatgttctt cattccaaaa ttaaagatca taaagaagat agaccttttt
     1681 ttgcaaccgt attaactctt accactcact atccatacag agccccaaga gaagaattta
     1741 atatttttgg gaaagaaaca gaggatagtg aatttttaaa tgtttatcat tacgcagatt
     1801 gggctgtgaa taatttttta gaaaaagcaa aacagtcctc ttattttgag aatactattt
     1861 tcttttttgt ggcagatcat acccatcacc gctttctcga ttattatgaa gatagaaata
     1921 ttccatttct aatttacagt ccttctcgtt ttaagggtga aattaggaat gatatagcct
     1981 ctcaactaga tgtaattccg acgatgcttg gcacattaaa caaggaagtt tactttagcg
     2041 caatgggaag agatttactc cactcgaaca caaactctgc ttatttcgcc tatggaactg
     2101 tttatggatg gatcgaagat aaattattcc tatttcgtac atctgatgga gaaggaaatg
     2161 ttaattttac ggtggatccc ccgcatttac aatttgatat ttgcaaaaaa actcctgatt
     2221 tttgcctaat tcataaccgt aaagcgaaag cgtttctaaa cacaagcatc gaactaatga
     2281 atcaaaatct aatatttccg gcagatatta aagatctaat gaatcagaac taatcctgcc
     2341 taacgtgccg ttatccataa gaaaacagga ttactaagca ctcaataact cgtaaaatta
     2401 gggatttgcg atgtaaacgc attacgttta tttacttgtg aaaaggatga aaggtaggtt
     2461 gccataggtt cactgcccca aaataaatgc aatcagttag tggcaaggta agattctaaa
     2521 tagaatttca atagatgatt ttcgaatctt aaaaataatg gactaaaaat cattcaggag
     2581 aaaaagataa tgaataaaag aatactttct attctttttt gtttagtttt tacagctaac
     2641 gtttctattt ttgcacaaga ggaatcaagc agttccggaa gtggctcagg tcaaacccaa
     2701 tctggtttaa caattaaagg tggtatatgg ggattaggcg gaaaagttaa atcagaatta
     2761 gaagataaat atttcacaga tgatttaatt agcaattttg gctttggaca gttcaatact
     2821 ggttggagca cacctagcaa aatctacccg ctcaatccaa taggcatcga ttattacatg
     2881 agtggtatag gtcctggatc tttactctta ggagcagaga tgagaggtat tcctggtgta
     2941 aatttcactg gatacactcc aaaatatgat tttgtaagtg taaacgctgg tggtgttgga
     3001 attcacaatg cagagatgaa attgaaaaat ttagacttaa atgtaggata ccaactcgct
     3061 ttcggaagtt tacttgttac tcctaaattc caatttagaa attttacaac agacttcaaa
     3121 gaatccggaa cttattttgg aaatggattt gttggtatca gaaatactac aaacaatcaa
     3181 aatacttggt caggatttat cggtgttaac ctcttatata aaatcaatga agcttcagca
     3241 gtattctttg attttgcaat cgattctcca atcctaggcc agctctatag cagtggagat
     3301 ttcaataaaa cagcattttt tgcaaacagc ggtggcgggg caagtggctc tacaactctt
     3361 tacaaaaaaa ccaatcaaga aattcgtgga acagtcattg atttaggata ccaacactct
     3421 ttcggagcgt taggtcttag aattggatat agatcagaag aacttagaac aatctattct
     3481 agttatactg atgtgccttt ggcatttaac tcaaatggaa atgctacatt tcttattggc
     3541 gagacaattc tcaattcaat cttctataaa ggatctacat ccacgactct acaaagtctt
     3601 tatataacag ctacctataa actctaatca atttcatttg atagaattat ccagacgagg
     3661 cgtaaactaa cgcttcgtct gttttaaaat tagtatttat tttactccac cacctactag
     3721 aatataagcc gcaacttgaa aaattccagt gattgcatct aaaggaattg tgagtatata
     3781 acccgcataa aacaaaccat acttgaatgt gtttataacc aaactccttt cccttctact
     3841 aatttggtct ctatcaaagc gatagtttgt tggaaccatt ttttttatat aaaaattgtt
     3901 atcttcttta cttctctcta attcaacaaa gattaaatca taatcaattc tataaaaata
     3961 cattttttcc tttaactctg actctgggat aaacatatcc ttcaggttta cttttctatc
     4021 ctccttgtta aacactttgc taaagctagt tggatcataa aataaaactg ccgctttatt
     4081 tccctgttga aattcatacg tatcaaaaac ttcagccttg gaatcatttg gatttatagt
     4141 tatatttaat tgccttcctt cccccactag aatagaatca aattgagctt tgactaaatt
     4201 gtcttttgga tcagctatat ttcttcctat agaaatcttc ttatcttttt gaaattctct
     4261 ttgataatca tcaatcccat agggctcaaa tctcttttga taagcataag agaaacaaga
     4321 atggagaaaa acgatagtta gaatgaatat cgttttacta aaaaaattta taatattgaa
     4381 atacttaaca cccattcatt tctccttttt tacgaatctt aaaaatacct tttcactctc
     4441 tggaagcatt ctagaagtag taaaagaaag cctgtaaaga ttcattttat tttcattttt
     4501 gtcgtaagta aaccgaaaaa gtatttttcc ataagagaaa gaagatattg gtttaggatt
     4561 aggatcctta atgggaagag ttaaattttc tgcatttaaa aactcttcta aatagttatt
     4621 ggatgcaaaa tcatttgtcc aaacataacc gctcataatc tcatcattaa ccggtagatt
     4681 aaataaattt ttatacccag ctttaccaaa actaacgtcc atagaagtgc caaatcctaa
     4741 aattttctcg tattttggtt ccgaacttgt tacaagataa atcatttctg attctgttgg
     4801 caatcgataa ctatcttccc ttcctgtctc cttgagcgaa attttcttta taaacaacat
     4861 tgcgtcttgg taaaaaatcc cataagcagg acactctggg caatcaggta acttatcttt
     4921 ttcccaagga gtaaaaaagt catttcccat atatttttcc caatattgtc tagtgatagg
     4981 atattttgta atcgaaaaag gagaaatacg aactgtaatt ttcttttctg gatttggact
     5041 tttggcagaa tgaaactggt aaaaataagt gtaatcaaac gaaaaatcac ctccacgaat
     5101 ttttacaaat tctagctttg gtaaatcctg tgaaaataac agagtggaag agagaagaaa
     5161 aataacattc cagataaaca tttttacctt cctttttatt tttagaaagc gagtattgaa
     5221 agaactcagt ttacaaaata ataaaaataa gtaaaacaat cttacaaaaa tacattgttt
     5281 caagcatata atattcaatt tatttcgaaa acaatattag cttttaaaaa agcatggggt
     5341 tttacgcatg acttctcagt tcttttacgg aagacaatca aagagcaaca aaaatatctc
     5401 taaatctaac tactcttcct cttttaactt aaaaaagatc aattttaatc tttttaaata
     5461 caaaccggta ttcgacccta aattctgtcg aatgattttt attataaaga caaaaacata
     5521 gattttttat tgatttatac agattctatt taatatttgt gtaaaataaa gtaggcaaag
     5581 ctagataaaa tttctactag ttccttgttt ggatcctcag aaatttttta ttacaatgat
     5641 atgaattcag aaagtcagca gaaaaacgaa gcacacagaa agattctttc taataaatct
     5701 gacacaacaa tttctaaaga agtatgtaag ctatgacacg aaaaaaaatt cttttagtag
     5761 aagatgatat catcatagct atggtagaaa ttgaacttct gaagagaaac aatttcgagg
     5821 tagttcacac agaaaatggg gaggaggctc tagataaagt tctctccaac gaaacaattg
     5881 atttaatttt aatggacttt gaacttggcg ctgggatgtc aggggcagaa gcggcagaag
     5941 aaatcctaaa aataaaaaac attccaatta tatttctcac ttcccatgta gaaaaagaaa
     6001 caattgagag agttcgaaac gtcaaccgtt atgggtatgt aattaaaaat tcaggagaaa
     6061 cttttctaat ttcttctatt gaaatggctc tcaatttatt cgaagcccaa agatctacca
     6121 aagaaagcga gcaaagattt agaaatctag cagattctgc ccctgtttta atttgggtag
     6181 cagatgaaaa taaaaactgg aattatttta ataaacaatg gctgatcttt agaggcagaa
     6241 gaatggaaga ggaaattggt cgtggttggc tttctggcat tcatcccaac gaccaagaaa
     6301 aatactttca attgtgtagt gatgcctttg aaaacaaaac tccgtatgaa actgaatata
     6361 gactccaaaa acatgacggt gtctatagat gggtattaga tagaggaatt cctcgttcgg
     6421 gagaaaatgg gcactttgca ggttttattg gctcggtcat agatataaca gatatgaaag
     6481 agcaagaaga caatttgcgt caggcggcag cggtgtttga taacacaaca gagggaattc
     6541 taatcacaga cgcgaataat tttatcaaag cagtaaatcc tgcttttaca tccgtaacag
     6601 gctattcgat agatgaccta atcgggaaaa ctcctaaaat tctaaattca ggaaaacaag
     6661 acaaaacatt ttatcaagaa atgtggaagt cattactcaa cacaggtaaa tggcaaggtg
     6721 aaatttggaa tcgtagaaag aatggagaaa tctatcctga acttttaggt atcagtgcag
     6781 tgaaagacag tcaaaataat acaattagct atgttgcagt cttttctgat atatcaaaaa
     6841 taaaagcagc tcaggatgat cttgattttc tagcacatca tgattggctc acaagcctac
     6901 caaatcgttt gatgttccaa tcaagactgc gacacgcaat gagcagatta gaaagtggaa
     6961 aacatctagc aattctttta atcgacttgg atcgatttaa agatgtaaat gatagttttg
     7021 gccatccgtt aggcgacgaa gtattacaac tctcagcaat tcgtatgcat gaaatcttat
     7081 ctgaaaaaga tacactcgcc cgattaggtg gggatgaatt tatagtatta atagaagact
     7141 tagacatttt agaaaatgca ggtcgtattg cacaggattt attagatgcg attgcaaaac
     7201 cattttacct gacaaatgga attgaagtat ttttgggggc aagtattgga attagtattt
     7261 atcccgacca agggcaatca gtagatgcaa tttttcaaca tgcagatgct gctctctacc
     7321 aagcaaaaaa agaaggcaag ggaatcttta gattctataa ctctggaatt acatcctcca
     7381 ttcgcattcg cctaacgatt gaaactagat taagaaaagc aatcagtaaa ggagaactgg
     7441 cagtatatta ccaaccacag gtagatatta gaacaggcaa agttgtagca gctgaagctc
     7501 tagtgagatg gatagatcct atagacggat ttgtttcccc ttctcatttt ataccgattg
     7561 ctgaagaaag tggactaatt agccaaattg gggaatgggt tttgagagaa gtttgcaatc
     7621 aaggaaatcg ttggctcgca gagggaaaaa ctttaattcc aattgcagtc aatctgtctc
     7681 caagacaatt tattcatggt aatattgtag gtttagtaga acaagtttta aatgaaacta
     7741 aatatccatc tgaattttta gaattcgaaa tcacagaaag tgccctgatg gatcgagaag
     7801 aagaagcaat taaaacactc aaccaactta ggaatatgag aattcgttta gccatcgatg
     7861 actttggcac cggctactct tcattagccc acttaaaaag atttccagta gatattttaa
     7921 aaattgataa gagcttcgtc gatgacattc cacaaaatga agacgataga gaaattgcag
     7981 ctaccatcat cgcaatgggt catactcttc gcctcaaagt tttagcagaa ggtgttgaaa
     8041 caaaagaaca actcgatttt ttaaactcgt gtggatgtga tctctaccaa ggcttttata
     8101 aatccaaacc tcttccggct gataaattta tagaattcat tcaagataaa atgtaaatct
     8161 agtccactta gctaacaaaa aaaccttgca aatgataaaa taataaataa gatcgctgtt
     8221 ctacatatga actggaatct atttattcga aatcaaaaca aaaaagcaat taggcaaaag
     8281 tctatatacc tttcgttgtg cttttctact cttttactaa cacattgtat gacgacagga
     8341 gagagaacca ttgaaggaaa cggagaaata gacaagtata acattagagg tttcactcag
     8401 tttactgttt atactggaag agattttctt ggatatgtca acgcagaagg acaagaaaag
     8461 acttttgata aaaaagtttg gcgaggtaaa gtaaaaaaag atacaataga acaatctatc
     8521 tctatatcct tttccaaaga agatttaaag gaattaaaag gtggatttgc agatatactc
     8581 tctgcagaaa ctggatacca atccatacag tccatgaatt taattttaga aaaacctgtt
     8641 caatacattc tagaagatat ttccatcgaa ccagaattta aaacaaaaaa agaattattc
     8701 acgagacaat acatcggagc agttctaaaa gtagataaaa ttagaatcga gtttcaaaac
     8761 aaaagcggct ttactttaaa aacagaagcc gacttccaag tgaaccaaat taaatttgga
     8821 aataatttca aacatggctc cacagaaaat tctgttttct ttgctgaaaa tgcatttata
     8881 ggttacaagt tatttaacgc accagaaaat cctgaatcat ttattgtaga agaggataat
     8941 cggattaaag ttgtagtcct accctttgaa gtagagggca caaaaacaaa agacgagaaa
     9001 ttaaaatcag cacttgcaga agcaacagca agatctctca acatggtgga taatttattt
     9061 gtattagata gaagtggcta tcaaaaaatt ttagaagagc aagaactagc taaagcagaa
     9121 aactatgatc aaaaaaaagc agtgcaaatt ggaaagctaa tgactgcgaa tacaattcta
     9181 atgggaaagt tttcaaaaga aggaattact gctcgtatta catccaaaat gattgatgta
     9241 gaaactaacg ctctactatc aaatggaacc atcaaattag acatcaatct aaaaggtaag
     9301 aatacaccca tgatagcaga tgagtgggag tctaaaattt tagaatctta tggagtaaaa
     9361 tcaaccaaaa gagattcaaa taacatatcc atccttggaa caaatgtagg cgatgcctat
     9421 gacttttatc gaagaggtag ggaaaattat attcttttag atgaagaaag tctagaatct
     9481 gcaattccat tatttagaaa agcaatcgaa cttgactcaa attattcgga tgcctatgca
     9541 atattagcag aaacgtaacc tatcaggctg ctaagttact tttatcaaga gaatataatt
     9601 ttaatata
//
LOCUS       DAOQOS010000175         9306 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_3970_length_9306_cov_6.210680, whole genome shotgun sequence.
ACCESSION   DAOQOS010000175 DAOQOS010000000
VERSION     DAOQOS010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 9306)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9306)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9306
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3970_length_9306_cov_6.210680"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(403..1020)
                     /locus_tag="PLF66_23745"
     CDS             complement(403..1020)
                     /locus_tag="PLF66_23745"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HMZ67123.1"
                     /translation="MYKLIIADDHPVIREGLKAYLLDSKSFSLEGVASSADELLRMIP
                     LFNPDVIMTDLDMPGSSTFDVLKKIKEDFPNIKKVVFTFHEEKSFFLKSVEASINGYI
                     LKSEPFEMISPLLLSIMEGGFAASPKMQKYLIAHSKDSVGYDSLTPREKEVLAYIARG
                     YSNIEIAEKFNLSVRTIEFHKNNIKEKLNMKSLAEIIKYYESLNV"
     gene            complement(1077..2972)
                     /locus_tag="PLF66_23750"
     CDS             complement(1077..2972)
                     /locus_tag="PLF66_23750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010409880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HMZ67124.1"
                     /translation="MDRLTSKNGFFVNKSGAVVLLRGVNLAGSSKVPLVPDGTTFLNQ
                     NESFQNHRNVSFVGRPFLEEEANEHFNRLRKWGFNFLRFIVTWEAIEHSGPGVYDDEY
                     LEYIVRMVKLAEKKGFYLFIDPHQDVWSRFTGGDGAPGWTLDAVGIDIMKIKDADMAY
                     IHHVQGKDYKKMIWPQNYNKYPTATLFTLFFGGNIFAQNLKIDGSNIQDYLQNHFIKS
                     IVRLAKKISKFKNVIGFGSLNEPSPGFIGRKNLTDFVGLGNGVLNISTPFQEMYCSEG
                     VPCNIDTKFLLGSIGVTVGKHVMNPNGISIWKKGHICVWRKHGVWNYDPNGAPMLLKS
                     DYFAKVGGRNVDFFSDFMKPFVKNFKTAIQKVNKQFFVFIESEPTKLELKWDEKEKKG
                     FAPVVNATHWYDGILLFTKRHFDWLGVHSFKMKIAFGRDSVQEMYNDSIASIKNMSLV
                     DMNQCPTVIGETGIPMDLEGANAYKTQDYSKQIYALDSIFKAIEKNLVNVAIWNYTPD
                     NTHKLGDCWNGEDLSIYSKDTPKQVCPDGGRGTKAFSRPYPILTEGNPVSLYFDISKG
                     LFKYSFRRGDFEKGSCVIYIPDVHYDTGLKVLVNAGTYTYKKEKNLLYFQGETGVDLY
                     GITILRA"
     gene            complement(2972..3844)
                     /locus_tag="PLF66_23755"
     CDS             complement(2972..3844)
                     /locus_tag="PLF66_23755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004460052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HMZ67125.1"
                     /translation="MKRGLILSGGGARGAYQAGVYKYLNERNFIPDVICGTSVGALNA
                     TALGCGFTPEKLIQLWRNIDAENVMRYSIWQNIKNIFLRKFTPMVDTTPLKNLLAQEL
                     DFRKLRTSSSKVYISAVNIKSSELTYFSNDEIDIQHLMASSAIPVVFPWQYVNGEPYW
                     DGGLMANTPIAPAIDEEAKDIVVVLLSPVGKVQMEMPKSRKEALERVFEMTLIASYQA
                     IRSNVEFTEPQPESKSFLSFLDYLIQSKNLRLRTVSPKNFLGLRSILNFSQLQADSLI
                     EMGYNDAKEQLGSI"
     gene            3929..4801
                     /gene="argB"
                     /locus_tag="PLF66_23760"
     CDS             3929..4801
                     /gene="argB"
                     /locus_tag="PLF66_23760"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016551066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HMZ67126.1"
                     /translation="MEESISKIKNILEALPYITEFSNKVIVIKYGGAAMVKEDLKESF
                     ARDIVLLKYLGIHPIIVHGGGPEINQILDVLKLPVKFVRGHRVTDEKTMEVVEMVLSG
                     KLNKQIVSLINSKSGQAIGLSGRDGKLALAEIQKIEVLTGEGKTELVDVGFVGRITKI
                     NKQLIQSLLDAKTIPVISPVAEDLNGQALNINADTMAGAIAGALNAEKLILLTDTPGI
                     LIKGKLAEFLNEAEVRELIQSGEISGGMIPKVECCLDAIQNGVKKTHIIDGRIQHSIL
                     MELFTESGIGTLIS"
     gene            complement(4790..5329)
                     /locus_tag="PLF66_23765"
     CDS             complement(4790..5329)
                     /locus_tag="PLF66_23765"
                     /inference="COORDINATES: protein motif:HMM:NF019235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1564 family protein"
                     /protein_id="HMZ67127.1"
                     /translation="MRKSENRKSSIIMQNKISFPNKNFNLEDFCSTLLIPERYIKVFV
                     KKVNQHEGVREYVSYLLAKYQIYIVNGLIPGYSNVTTKYQEKGQNLQRVSFRPDATDW
                     AELKIYRLTFGMSISAFIVYLLIADSVEFEKTVSNIFDIVGILEMAILNLDARVSLSN
                     NRTNYSIHLHYRQNLQSTY"
     gene            complement(5397..5906)
                     /locus_tag="PLF66_23770"
     CDS             complement(5397..5906)
                     /locus_tag="PLF66_23770"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HMZ67128.1"
                     /translation="MIDFSNIYNFKKVPNQEDGTCFACSPINDKGLKMEFYTDEKSVF
                     SKLVVPTHLSGWSNVVHGGVTTTILDETIAWAVIYLRQSYMLTKSISVEFLQPLHTGE
                     ELYSYGRIIEFRSERELVVEAFVFKKENLLAAKAVGTTILFSSEQMRKRGIFPESFLN
                     DFEKKVFKK"
     gene            complement(5911..6594)
                     /locus_tag="PLF66_23775"
     CDS             complement(5911..6594)
                     /locus_tag="PLF66_23775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016550094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HMZ67129.1"
                     /translation="MQSVNRRKIGNLNVYEVKGKEGGPVIILLHGFGANGQDLLPLHE
                     YIKAPAGANWYFPDAPIEMDLGGGYKGRAWFPLMSSAIEHVAKYGMDFSNLHPPGIDS
                     ANAEILKLIEEIGVPTEKITLGGFSQGSMLATDVTLRLNENMAGLVILSGTLISKSEW
                     APLVKNKSRIQFFQSHGTEDPVLLYQGGKNLESLLRENGMSGEFVSFNGGHEIPDKVL
                     KRLSQYLLR"
     gene            6679..7047
                     /locus_tag="PLF66_23780"
     CDS             6679..7047
                     /locus_tag="PLF66_23780"
                     /inference="COORDINATES: protein motif:HMM:NF008740.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HMZ67130.1"
                     /translation="MSLLGNIIWLICGGFLSGLGYIIGGLSTCLTIIGIPFGLQAIKI
                     GIATMAPFGKENVVTDKSESVIRLIFNIIWLFLFGWAIALAHLTSAGLLAITIIGLPF
                     AYQHLKLIPLALFPFGRELK"
     gene            complement(7068..7370)
                     /locus_tag="PLF66_23785"
     CDS             complement(7068..7370)
                     /locus_tag="PLF66_23785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67131.1"
                     /translation="MPEVEVKSKEELCVRLGEFLSIYHKAKIIEPKEDTLKMYILLSK
                     NKNGTPKEKLINFKLLRVDERLFPVDKQELSPKDAIICEFLIEELHKYVARQNKPK"
     gene            complement(7370..8152)
                     /locus_tag="PLF66_23790"
     CDS             complement(7370..8152)
                     /locus_tag="PLF66_23790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016551438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HMZ67132.1"
                     /translation="MDTLYIVLIAWLVVFFIMSGLWFLGKQIKNYAIVDVGWAFSLVA
                     ICLVYYILCDGYAPRKSIIMFMVFFWGFRLSSYLAVRVLSHGEDARYTEFRKDYGDAV
                     DRKFFTNIFQFQGLLDIILSIPFLIIASNSDIEIHFLEYLGLIVFLVSVIGESVADFQ
                     LSQFKSNPANKGKTCQIGLWNYSRHPNYFFEWMIWVSYFIVALPSPNGYLAVIPAILM
                     LIFLLKFTGIPMTEANALKSRGDEYREYQRTTSAFVPWFKKK"
     gene            complement(8155..8628)
                     /locus_tag="PLF66_23795"
     CDS             complement(8155..8628)
                     /locus_tag="PLF66_23795"
                     /inference="COORDINATES: protein motif:HMM:NF021363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HMZ67133.1"
                     /translation="MIKSFLKNKVGKPIVNLLKQGITPEKISLCIALGGMLGVFPVLG
                     STMILCTIASFAFKLNLPLIQLISYLVYPLQILFLIPFIRFGEWVLNVPPFPISIEAL
                     VDMIKTDIWQTIVTFWDATMHGIFGWMIVACIMIPLLYFIFLPILKRIPMKGIKE"
     gene            complement(8625..8954)
                     /locus_tag="PLF66_23800"
     CDS             complement(8625..8954)
                     /locus_tag="PLF66_23800"
                     /inference="COORDINATES: protein motif:HMM:NF018991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1475 family protein"
                     /protein_id="HMZ67134.1"
                     /translation="MILFLKILFSLILASMLWVTTWASLQINLFTYLPTLVKDPWVIA
                     TLFDAYYGFLTFFLWVCYKENSIVSKLLWFIAIMLFGNITMAIYVLIQLFKLEKDGTM
                     KDLLIQK"
     gene            complement(8956..>9306)
                     /locus_tag="PLF66_23805"
     CDS             complement(8956..>9306)
                     /locus_tag="PLF66_23805"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010568252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HMZ67135.1"
                     /translation="CPDSRYEQFRTGIDFIQKYIFPGSLLPSIGRINEAINRTSNLFL
                     FHLEDMGLYYAKTLKLWLDGFDKNIGKVRELGFDEKFIRTWRYYLSYCNAAFKMRNIS
                     VVQAVYTRPNNMEI"
BASE COUNT         3207 a         1715 c         1404 g         2980 t
ORIGIN      
        1 tatgatgttt tcatcaacat acataataaa agaatatttt tgaatgattt catcgtttaa
       61 atcaacaccg ctaacattgg tctgatacat ttcataaatt ttattacatt gagacagaca
      121 aaggagaaaa taaaaataga ttaaaatttt tttcatagga atacttaaaa actaatcggt
      181 tataaataag taggtcaaga ataatttaag gctattataa agagaaggtt acagttggaa
      241 gataaactag gatttctttt actaatttac taaacacatt gaaatcagag tagaaggttt
      301 aggcaaatta gtaaaagaaa tgaattaaaa gtaaaaatca ttaaggatga tcaatctaat
      361 tctttaaaga tatattttac aaagcattgt tatttctatt cattaaacat tcaaggattc
      421 ataatattta atgatttctg ctagactttt catatttaac ttttctttaa tattgttctt
      481 gtgaaattca atcgttctaa cagataaatt aaatttctca gcgatttcaa tattagaata
      541 gccacgagcg atgtaggcta atacttcctt ttctcgtggg gttaatgaat cgtaacccac
      601 agaatctttt gaatgcgcaa tcaaatactt ttgcattttt ggagatgcgg caaacccacc
      661 ctccataata gacagaagca atggtgaaat catttcaaaa ggctcagatt ttagaatgta
      721 tccgttgata ctagcttcaa ctgattttag aaaaaaagat ttctcttcat gaaaagtaaa
      781 aacaactttt tttatattag gaaaatcttc tttaattttt ttgagaacgt caaatgtgct
      841 agacccaggc atatccaagt ctgtcataat aacgtcagga ttgaataagg gaatcattct
      901 taaaagttca tcagcgctag atgcaactcc ctctaaagag aaacttttag aatccaataa
      961 atacgctttt aaaccttcgc gaattactgg atggtcgtca gctatgatta atttatacat
     1021 aatttagaat tgattggata agttattttt tacaagtaat ttttatgaat attgagtcag
     1081 gctctcagaa ttgttattcc atacaaatct acaccagtct ccccctgaaa atagagcagg
     1141 tttttttctt ttttataagt atatgttcca gcattgacca agaccttaag cccagtatcg
     1201 taatgaacgt caggaatata gataacacat gatccttttt caaagtctcc tctcctaaaa
     1261 gaatatttaa aaagtccctt cgaaatatca aaataaagtg atactggatt tccttccgtt
     1321 agaataggat aaggtcttga aaatgctttg gttcctcttc caccatcagg acaaacttgc
     1381 ttaggagtat ccttagaata aatagaaaga tcctctccat tccagcagtc tcctagctta
     1441 tgagtattat caggtgtata attccaaata gctacattca ctaaattttt ttcaattgct
     1501 ttaaagatcg aatcaagtgc ataaatctgt ttgctataat cttgcgtttt atacgcgtta
     1561 gccccttcca gatccatagg aataccagtc tctccaatga ctgtaggaca ttgattcata
     1621 tcgactaacg acatattttt gattgatgca atggaatcat tatacatttc ttgaacagaa
     1681 tctcttccaa atgcaatttt cattttaaaa ctatgaacac ccaaccaatc aaaatgcctt
     1741 tttgtaaata gtaaaattcc atcataccaa tgagtagcgt ttacaacagg ggcaaagcct
     1801 ttcttttcct tctcatccca ctttagttct aacttagttg gctcactttc tataaacaca
     1861 aagaattgtt tattgacttt ctgaatcgct gttttgaagt tttttacaaa tggtttcata
     1921 aaatcagaaa agaaatcaac attccttcct ccgaccttcg cgaaatagtc agattttaga
     1981 agcataggag ctccatttgg atcataattc caaaccccat gcttgcgcca aacacagata
     2041 tgccccttct tccatataga gataccatta ggattcatta catgtttacc aactgtaact
     2101 ccaattgatc ctaatagaaa tttcgtatca atgttacaag gcactccctc agaacaatac
     2161 atttcctgaa aaggcgtaga aatattcaaa acaccattgc ctagccccac aaaatcagtt
     2221 aaattttttc tacctataaa tcctggagag ggttcattta aacttccgaa tccgattaca
     2281 tttttaaact ttgaaatctt cttagctaaa cgaacaattg atttaataaa atgattctga
     2341 agatagtctt gaatattact cccatctatt ttaagatttt gagcaaaaat attacctcca
     2401 aaaaataaag taaataaggt tgctgttgga tatttattat agttttgagg ccagatcatt
     2461 ttcttataat ctttaccctg gacatgatga atatatgcca tgtctgcatc cttgatcttc
     2521 atgatatcta ttcccactgc atctaacgtc caccctggag ctccatcacc ccctgtaaat
     2581 ctagaccaaa catcttgatg aggatcaata aataaataaa accctttctt ttcagcaagc
     2641 ttaaccattc gaacaatata ctctaagtat tcatcatcat aaacccctgg tccggaatgc
     2701 tcaatcgctt cccaagtgac tataaatcga agaaagttaa atccccactt tctcaatcta
     2761 ttaaaatgtt cattcgcctc ctcttctaaa aaaggccgtc ccacaaaaga tacgtttcgg
     2821 tgattttgaa aactttcatt ttgatttaaa aatgttgttc catcaggaac aagtggaact
     2881 tttgaacttc cagctaaatt cactcctcgt aataaaacta ccgctccaga tttattaaca
     2941 aagaagccat tttttgacgt tagtctatcc attagatgct acctaattgc tctttggcat
     3001 cattatatcc catttcaatc agagaatctg cctgcaactg actaaaattt aaaatacttc
     3061 taagtcctaa aaaatttttc gggctaacag ttcttagtct caaattttta ctttgaatca
     3121 aataatccag aaaagatagg aaactctttg attcgggttg tggctctgta aattctacat
     3181 tcgaacgaat cgcttgatag gatgcaatta aagtcatttc aaaaactctc tcgagcgctt
     3241 cttttctaga tttaggcatc tccatttgga cctttcctac tggagaaagt agaaccacaa
     3301 ctatatcctt tgcttcttca tcaattgcag gtgcgattgg agtattagcc attaaacccc
     3361 catcccaata aggttcacca tttacatact gccatgggaa aacgactggt attgcggatg
     3421 acgccataag atgttgaatg tctatctcat cattagaaaa ataagttagt tcggagcttt
     3481 ttatatttac agcagaaata taaaccttag aggaagatgt tctgagtttt cgaaaatcta
     3541 attcctgtgc taataaattt tttaaaggtg tagtgtctac cattggtgta aatttacgta
     3601 gaaaaatatt tttaatattc tgccaaatgg agtatcgcat tacattctct gcatcaatgt
     3661 ttctccacaa ttgaattaat ttctctggcg taaatccaca acccagagct gttgcattta
     3721 atgcaccaac cgatgttcca caaataacat caggaataaa attcctttca tttagatact
     3781 tataaacccc agcttgataa gcacctctag ccccaccacc tgataaaatt aaacctcttt
     3841 tcatacatcc aatctgacaa aataactcct cctctcaaga ataaaaaaaa cttttagtat
     3901 attgaggatt tgaaaactta ggtttactat ggaagaaagt atttcaaaaa ttaaaaatat
     3961 attagaagca ttaccatata taaccgaatt ttcaaacaaa gttatagtta ttaaatatgg
     4021 tggagcggca atggttaaag aagatttaaa agaatcattc gccagagata ttgttctttt
     4081 gaaataccta gggatacacc cgattattgt gcatggcggt ggtcccgaaa ttaatcaaat
     4141 cttggatgtt ctaaaactac cggtaaaatt tgtgagaggt catagagtaa ctgatgagaa
     4201 aactatggaa gttgttgaaa tggttctctc aggaaaatta aataaacaaa ttgttagtct
     4261 tataaactct aaatccggac aagccattgg attatccggt agagatggca aattggcatt
     4321 agccgaaatt caaaagatcg aagtcctaac tggagaaggc aaaacagaat tagtcgatgt
     4381 agggtttgta ggaagaatta caaagataaa caaacaatta attcaatcct tgctagatgc
     4441 aaaaacaatt cctgtaattt cgcccgtcgc tgaagattta aatggtcagg ctctaaatat
     4501 caatgcagat acaatggcag gtgcgatagc cggtgcatta aatgctgaga aattaatttt
     4561 actaacagac actcctggta ttctgataaa aggaaaactg gctgaattct taaatgaagc
     4621 agaagtcaga gaactaatcc aatcaggaga aatatctggc ggcatgattc caaaagttga
     4681 atgctgttta gatgcaattc aaaatggtgt caagaaaaca cacattatag atggaagaat
     4741 tcaacattcc attttaatgg agttatttac ggaatctggt attggaactc taataagttg
     4801 attgcaagtt ctgccgataa tgcaaatgaa tagaataatt ggttctattg tttgaaagac
     4861 tgactctagc gtctaagtta agtattgcca tctcaagaat tcctacaata tcgaaaatat
     4921 tggatacagt cttttcaaat tccacagagt ctgctattag tagataaact ataaatgccg
     4981 aaatagacat accaaaggtt aagcgataga tctttaactc tgcccaatca gttgcatcag
     5041 gacgaaatga gacacgctgc agattttgac ctttttcttg atatttagtg gtaacatttg
     5101 aataaccagg aatcaatcca ttgacaatat aaatttgata cttagccaat agataactaa
     5161 cgtattctcg aacaccttca tgttgattaa ctttttttac aaataccttt atatatctct
     5221 ctggaatcaa taaagtcgaa caaaaatcct caagattaaa attcttattt ggaaaagaaa
     5281 ttttattttg cataataata ctagattttc tattttccga ttttctcatt ttatttacct
     5341 catataatat aatgaggaga aaatctttgt ttttgagcag gtttttttat attattttac
     5401 tttttaaaaa cttttttttc gaagtcattc aaaaaagatt ctgggaaaat gccacgttta
     5461 cgcatttgct cggacgaaaa gagtatcgta gttcctacag cctttgcggc caataggttt
     5521 tccttcttaa aaacaaatgc ctccactact aattctcttt ctgatctaaa ttcaataatt
     5581 ctaccatacg aatacaattc ttctcccgta tgtaaaggtt gtaggaactc cacagatata
     5641 cttttggtta acatataact ttgacgaagg tagatgactg cccaagcaat cgtctcatct
     5701 agaatggtag ttgtaacacc accatgcaca acattactcc agccagatag gtgtgtagga
     5761 actactagct tggaaaaaac agatttttca tctgtataaa attccatttt taatcctttg
     5821 tcattaatag gactacaggc aaagcaagtc ccatcttctt ggtttggaac tttcttaaaa
     5881 ttgtaaatat ttgaaaaatc aatcataaat tcatctcaat agatattggc tcaaacgttt
     5941 tagaacttta tctggaattt catgacctcc attaaatgaa acaaattctc ccgacatacc
     6001 attttctcga agaagagact ccagattttt tccgccttga taaagaagga ctggatcctc
     6061 tgttccatgg ctttgaaaaa attgaattct agatttattt ttgacaagcg gtgcccattc
     6121 tgattttgaa attaaagttc ctgaaagaat taccaatcca gccatgttct cattgaggcg
     6181 taaagtaacg tcagtagcaa gcatagagcc ttgactaaat ccgccgagtg tgattttttc
     6241 tgtaggaact ccaatctcct ctattagctt taatatttcc gcattggcag agtcaatgcc
     6301 aggaggatga agatttgaaa aatccatacc atattttgca acgtgttcaa tagcggaaga
     6361 cataagcgga aaccaagctc tacctttata accgccacct aagtccatct caattggtgc
     6421 gtccggaaaa taccaattag ccccagctgg agctttgata tattcatgaa gaggaagaag
     6481 atcttgtcca tttgctccaa acccatgtaa tagtatgatg acaggaccgc cttcttttcc
     6541 ttttacctca tacacattta aattaccaat ttttcttcta ttcacagact gcatgattaa
     6601 ataatttgaa atcagtatgc agtgtcaagt cattcgaagg atgtatagga aagcatatct
     6661 aataattagg gagatgatat gagtttactt ggaaatatta tttggttaat ttgcggagga
     6721 tttttatcag gtcttggata cataattggt ggactaagca cttgccttac aatcattgga
     6781 atcccttttg gtttacaagc aatcaaaatt ggcattgcaa caatggctcc ctttggaaaa
     6841 gaaaatgttg taactgacaa aagtgaaagc gttatacgcc taatattcaa tataatttgg
     6901 ttgtttttat ttggctgggc tatcgctctt gctcatttga cttccgcagg acttttggct
     6961 attacaatta tcggtctgcc gtttgcttac caacatttaa aattaatccc acttgcctta
     7021 tttccctttg gaagagagtt aaaataaagc ttatttatat ataagcttta tttcggtttg
     7081 ttttgtctgg ctacatattt atgcaattct tcaattaaga actcgcagat aattgcatcc
     7141 ttcggggata actcttgttt gtcgacagga aacaatctct catctacacg taatagttta
     7201 aaattaatca atttctcttt cggagttcca tttttatttt tagaaagaag aatatacatt
     7261 ttcaaagtat cttcctttgg ctcaatgatc tttgccttat gataaataga caggaactca
     7321 cctaacctaa cacaaagttc ttctttagat tttacttcta cttcaggcat tatttctttt
     7381 taaaccaagg gacaaatgct gaagttgttc tttgatattc tctatattcg tcacctctag
     7441 atttaagtgc atttgcctct gtcattggga ttccggtaaa tttcaaaaga aaaatcaaca
     7501 taagaatagc aggaattaca gctaaatatc cgttaggcga aggaagtgct acgataaaat
     7561 acgataccca gatcatccat tcaaaaaaat aattaggatg acgagaataa ttccaaagcc
     7621 ctatttgaca agttttccct ttattagcgg gattcgattt aaattgactc aattgaaaat
     7681 cggcaacgct ttcaccaata acagatacca gaaatacaat taagcctaaa tattccaaaa
     7741 agtgaatttc tatatccgaa ttacttgcaa ttataagaaa cggtatagag agaataatat
     7801 caagtaaccc ctgaaattga aatatattcg taaaaaattt tctatctacg gcatcaccat
     7861 aatcttttct aaattcagta tagcgagcat cctcaccatg actcaaaact cttaccgcca
     7921 aataagaact caatctaaat ccccaaaaga aaaccataaa cataataatc gattttctag
     7981 gagcgtagcc gtcacaaaga atataataaa caagacatat tgcgacaaga gaaaacgccc
     8041 atcctacatc gactatcgca taatttttaa tttgtttccc taaaaaccaa agtcctgaca
     8101 tgataaaaaa aacaactaac catgcaatca acactatata taatgtatcc ataattactc
     8161 ctttattcct ttcattggaa ttcttttaag tatgggaaga aaaataaagt ataagagtgg
     8221 aatcattatg caagcgacta tcatccatcc gaaaatacca tgcattgtag catcccaaaa
     8281 cgtaacaatt gtctgccata tatccgtttt tatcatatcc acgagagctt caatggagat
     8341 aggaaaagga ggcacattca aaacccattc cccaaatcga ataaacggga ttaaaaatag
     8401 aatttgcaaa ggatatacca aatagctgat caattggatc aaaggcaaat ttaatttaaa
     8461 tgcaaaactt gcaatggtgc aaagaatcat agttgaacca agcacaggaa aaactcctag
     8521 catgccacct aacgctatac aaagagaaat cttctctgga gttattcctt gcttcaaaag
     8581 atttacaatt ggcttcccaa ctttattttt taaaaatgat tttatcattt ttgaataagt
     8641 aaatctttca ttgttccatc tttttctaat ttaaagagtt gaattagaac atagatagcc
     8701 attgttatgt ttccaaacaa cataatcgca atgaaccata gcaatttaga aacaatagaa
     8761 ttttctttat aacaaaccca aaggaaaaat gttaaaaacc cataataagc atcaaataga
     8821 gtcgcaatta cccacggatc tttaactaat gtaggcaggt aggtaaaaag attaatttga
     8881 agagatgccc aagttgttac ccaaagcatc gaagcaagga tcaaactaaa aagaattttt
     8941 aaaaataaaa tcatactaaa tctccatatt atttggtctg gtataaactg cttgcacaac
     9001 agaaatattt ctcattttaa atgctgcatt acaataagat agataatatc tccaagtgcg
     9061 aataaatttt tcatcgaacc ctaactctct tactttacca atattcttat caaatccatc
     9121 tagccaaagt tttaaagtct tggcgtaata taagcccata tcttctagat gaaataaaaa
     9181 taaattacta gtccgattga tcgcctcatt gattctaccg atagacggta gaagagaacc
     9241 cggaaaaata tatttttgaa taaaatcaat tcctgttcta aactgttcat aacgggagtc
     9301 aggaca
//
LOCUS       DAOQOS010000176         9239 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_4010_length_9239_cov_7.316202, whole genome shotgun sequence.
ACCESSION   DAOQOS010000176 DAOQOS010000000
VERSION     DAOQOS010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 9239)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9239)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9239
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4010_length_9239_cov_7.316202"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(174..1415)
                     /locus_tag="PLF66_23810"
     CDS             complement(174..1415)
                     /locus_tag="PLF66_23810"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing methyl-accepting
                     chemotaxis protein"
                     /protein_id="HMZ67136.1"
                     /translation="MLEFITKYFQKDKARIKALEEENKLLKTFFWHLPHISFVRDKDG
                     KFVLVNQNFAKNFKASSMEEIIGKTDFDFNPNKEQVEKIQKQDKEIMASKSKFMPPIT
                     KYLDKEGKATYLETIKIPVIEANDLSSNILGFSINVTEKMELQNRAEEAMNKLLTAVT
                     DVTEKIELILKQANAVVNSTKKQAENLSFLTEIAHQIIESNAQSIQMMSAVLYIVTNT
                     SDIADQGGKYLELMLQSMEKIQENAKQMQSIIEIIDTIADQTNLLSLNASIESARAGE
                     AGLGFSVVAREISKLAEKSAESTKGINALVKQTNQIIQKGNENVTEGGETFKKIISEV
                     GNIDTLINSVDETMKGQTQIYNTFREKIEEINNEATQINHITAEQMNHVNSVMASINQ
                     LNRDFINLLTVQSKNGNGHGK"
     gene            complement(1621..1896)
                     /locus_tag="PLF66_23815"
     CDS             complement(1621..1896)
                     /locus_tag="PLF66_23815"
                     /inference="COORDINATES: protein motif:HMM:NF021758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SelT/SelW/SelH family protein"
                     /protein_id="HMZ67137.1"
                     /translation="MKEPRIEIEYCTQCNWLLRASWMAQELLTTFSEEIGELVLIPGK
                     NGILEIRVSGQILFSRKQEGRFPEMKEIKQLVRDKIAPEKKLGHSDK"
     gene            complement(1897..2838)
                     /locus_tag="PLF66_23820"
     CDS             complement(1897..2838)
                     /locus_tag="PLF66_23820"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HMZ67138.1"
                     /translation="MDEFSKIIINAVNKAKSSVVKIDTYKTINGKETPSGSGSGFLFS
                     TDGYLFTNSHVVHGGSKFRTTLYDSSQYEAILIGEDPDSDLAVLKTFATEYQTVKLGD
                     SKDLEIGQLVIAIGNPHGFQHTVTHGIVSANGRTLRTQSGRLIDNIIQTDAPLNPGNS
                     GGPLIDADGNVIGVNTATIMGAQGLCFAIGINTAKDIAVELIQTGKVKRAYLGIMTQI
                     VELNSKVMVYNDIRNKKALFVISVEPGSPAARAGLRDGDYILSFNNEKVESTDDLFRM
                     LTREKIGEVHNIGILRKNQKVILNILPMENIKLVSNG"
     gene            complement(2856..3578)
                     /locus_tag="PLF66_23825"
     CDS             complement(2856..3578)
                     /locus_tag="PLF66_23825"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HMZ67139.1"
                     /translation="MSKKILITGANRGLGFAFVKVSLDNGYTVLAGCRNPEKAEDLKQ
                     LRSEFTERLHIINLDPTNINSIKQVVEYAKQHFGSLDILVNNAGMNSKSSGVDPSIHL
                     QLGKFQAEPILNMFHLNAVAPLIMAQEFLDLLSKSQRGLIVNISSWFASLSIPQKGNY
                     SYSASKAALNMFNKVLANDLGKYGILTLAVNPGWMKTDMGGKSASLEPEESAKSILEF
                     SQKLKMEDSGKFFNHDGSIHLW"
     gene            complement(3650..4066)
                     /locus_tag="PLF66_23830"
     CDS             complement(3650..4066)
                     /locus_tag="PLF66_23830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004432789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HMZ67140.1"
                     /translation="MTSRFTVAIHILSLLDLNQGQSRTSEELAVSVNTNPVVIRKILG
                     ILKKAGFIHIQMGQGGGATLAVDSEKLTLKDVYRVFNERLFAMHASKPNTKCICGRNI
                     QPILTDVFTETEQIVISELSHRTIHQITQEILKRAK"
     gene            4235..5896
                     /locus_tag="PLF66_23835"
     CDS             4235..5896
                     /locus_tag="PLF66_23835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5982 domain-containing protein"
                     /protein_id="HMZ67141.1"
                     /translation="MKNKINFKIFFYIMTFVAISGNVFAQDAKDAEKNPTQTNQREPV
                     KGLPFEISEQKRLSEADVSKKKEGWFPTGVGGPFSDPNNGAGVGGRIFLFNNGKKTDP
                     FFEYTPYRHRIFLNLSNTTRNAQYHQLDWDAPYIFDTKWRARANAIYDRNPNNLFFGL
                     GESTMKGLSYHPRNDYSQPLVTNGTFPEMQEAQEFRRPPNAGEPQYLSDNFYNSALLA
                     NNPVPGMRNQYVTDKKYNRYDLEVPQVNLSAENSFFGGVMRLVVGTKLSNNIVRRYDG
                     SIQKANDSFFRDTPLEYQHTVGLGGTPFELNGISANQGNIPTVNGKTKITEEYERGRI
                     KGINGGYVNILRFALVYDTRDFEPDPSRGIFAELTHERSSKAIGSNFEFNRTLGSVRF
                     FYSPFPKVFKKLVLAGRGVFVHNSGNLPFFEYRNMWSTEGGLSGLGGRTTLRGYIQDR
                     FVGTNMGFANLELRWRFFEIPGFTFNIAPLFDLGRVWDTLGSVKANAWDGYKYSYGLG
                     LRIIWNQSTVIYAEWARSRETRTSGWPADAPFSNTNFYFNFGHIF"
     gene            5907..6599
                     /locus_tag="PLF66_23840"
     CDS             5907..6599
                     /locus_tag="PLF66_23840"
                     /inference="COORDINATES: protein motif:HMM:NF019237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HMZ67142.1"
                     /translation="MKKLMQYSFLSICLIILISCEAKPEKSYFGVPEEDGKQILAGLI
                     LGNGSIDNATGTVVDPKTGLEWKKCTQGQVFRSTNNDCQGSRATSTLTTPIDQGRYGA
                     TYLAYCNIEGNDCNTSSLPMALKSNTVTGVFSEAFLSCNTDRTNGYTNWRVPSYPELQ
                     ALASLGKVVLTNRFPNTPDDYFWSSWSNEQDTSGKTARAVSFSLETIGKDQNISKTTR
                     FYVRCVRNINGQ"
     gene            complement(6742..8301)
                     /gene="gltX"
                     /locus_tag="PLF66_23845"
     CDS             complement(6742..8301)
                     /gene="gltX"
                     /locus_tag="PLF66_23845"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016753392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HMZ67143.1"
                     /translation="MNQNVRTRFAPSPSGFLHVGGARTALFNYLYAKATGGKFLVRIE
                     DTDQDRSTDSSMKIILESLEWLGLNWDEGPGVGGPHGPYKQSERLNIYKEYTERLIQN
                     NHAYRCFCTTELLESKKKQSEAMGIPNIYDGTCSSMTEKEIQEKLDQKIPFSVRFRTP
                     AKNLIVDDIIQGKVKFDTKLIGDFIIVKSDGFPAYNYAVVVDDHLMEITHVIRGVGHL
                     SNTPRQILIYEAFGFPLPKYAHASEIVGADGKKLSKRAGATSILAFRDLGYTAECFRN
                     YMALLGWTSESGQEYLPKGELEKLFDVERCSKSPSLFDVFKKVKEEDKEKLDFNSLPL
                     SALADQLNPKSKLNWLSNKYIRDSDIVKLSGEILPFIKDNNSIPDDIKQAENKTLQSI
                     LESIRVYLDRLSQAPDYISEFFNNDIVIENEEARTIATSGNADKVIQTFFKLLGNGEP
                     KSSDEYKALIDKTGIETGEKGKNLYMPIRVATTGKAHGLELPVLFNLLGTEKLKYRIK
                     VISKEIGLSVS"
     gene            complement(8285..>9239)
                     /locus_tag="PLF66_23850"
     CDS             complement(8285..>9239)
                     /locus_tag="PLF66_23850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67144.1"
                     /translation="GLFFIFKNLQKPLILLTQMIHNRVANFFLLFFIIFQSACSSIGN
                     NLLENRKFLEGFIGDYYQNAKVLNTNSFFEKQSSHYKYLVIRQNENGKLSVREIDISF
                     YAYTNKFKEDVTRLFFDKAPPKYNIYEKVKYGEGQRKIDSNDLEVEYTNFSIRDAQST
                     DLLSAIDKFISSKTDAQQLFHEKVTYSQTPDEGLWKLEEVHLKDGQEAIRWKHYPSGT
                     VARNYPHTEFSPNKNTLEYSRIYAFEDYSYKKIFKDIPSAIKLYNSKDYTLYYYPMEI
                     LDAQNESLISSGKNQQLSLYKIKLPIIIYKKKESMTDESKR"
BASE COUNT         2788 a         1630 c         1542 g         3279 t
ORIGIN      
        1 atgaccactt caattctatt ttacaaacgt gcgcgacttg acaaagtcgc attaaattag
       61 agagaatttc tgttatttgg ctgagaggga aaagatgcaa gtgtgagaaa tttccctaat
      121 gaataagagg aaaaaaatct aaagaatgtg cgctattttt ttaaaaaaaa ttattattta
      181 ccgtgtccat ttccattttt gctttggacc gttagcaaat tgataaaatc tcgattgagt
      241 tgattaatag aagccattac agaattaaca tgattcattt gttcagctgt gatatggttg
      301 atttgtgtag cttcattatt tatttcttca attttttctc gaaaggtatt gtagatttgg
      361 gtttgacctt tcattgtctc atcgacagag ttaattagag tatctatatt tcctacttca
      421 ctgatgattt ttttaaaggt ttctcctccc tctgtaacat tttcatttcc tttttgaata
      481 atttgatttg tttgttttac caatgcattg atgccttttg tgctctctgc agatttctct
      541 gcaagttttg aaatttctct tgcaacgact gaaaatccta aaccagcttc tcctgctctt
      601 gcagactcaa tacttgcatt gagagaaagt agatttgttt ggtctgcgat tgtatcaata
      661 atttcaataa tactttgcat ttgttttgcg ttctcttgaa ttttctccat tgattgaagc
      721 attaactcaa ggtattttcc gccttgatct gctatatcac ttgtgttagt aacgatatac
      781 aaaactgcgg acatcatttg aattgattgt gcgttagact ctataatttg atgtgcgatt
      841 tctgtaagga agcttagatt ctcggcttgt ttttttgtgc tatttacaac tgcatttgct
      901 tgtttcaaaa ttaattcgat tttttcagtt acatcagtca ccgctgttaa aagtttgttc
      961 atcgcttctt cggctctatt ttgtaactcc atcttttctg tgacgttaat cgaaaaacct
     1021 aaaatgtttg aagagagatc gtttgcttct attacgggga tttttatagt ttctaaataa
     1081 gtcgcttttc cctctttgtc taaatacttt gtgatgggag gcataaactt acttttagat
     1141 gccattattt ctttatcttg cttttgaatt ttttcaactt gttctttgtt aggattaaaa
     1201 tcaaaatccg ttttaccaat tatttcttcc atggaggagg ctttgaaatt ttttgcaaaa
     1261 ttttgattta ccagaacaaa ttttccatct ttatctcgca caaaagaaat atggggcaaa
     1321 tgccaaaaaa aagtcttcag aagtttgttt tcctcttcta atgctttgat tctagcctta
     1381 tccttttgaa aatatttagt aataaattct aacatgcgat ttttgcttct tataatatta
     1441 acgatacttc aagcatttat ttaaactaat aatttaaatg atgattttga agatatttat
     1501 agataaataa tttattgatc ggaagatgaa ataaattcta tacctttatt aagattctga
     1561 aagaagcagt tataggattg caagaaaaaa aacattggag tttatttttt ctatcctact
     1621 ttatttatca ctgtgcccta attttttttc tggggctatt ttatctctta ctaattgctt
     1681 aatttccttc atttcaggga aacgcccttc ttgtttacgg gaaaacaata tttgtccgct
     1741 cactctaatt tctaaaattc cattctttcc aggaattaga accagctccc caatttcttc
     1801 tgagaaagtt gtgagtaatt cctgagccat ccaactggct cttagaagcc agttgcattg
     1861 cgtgcaatac tcaatctcta ttcgaggttc tttcatttat ccattgctca ccaatttaat
     1921 attttccatc ggcaaaatat tcaaaatcac cttctggttt tttctgagaa ttcctatatt
     1981 atgaacttca ccaatttttt ctctcgtaag cattcgaaac aaatcgtctg tagattccac
     2041 tttttcattg ttaaacgaaa gaatataatc tccatctcgc aagccagctc ttgctgctgg
     2101 acttcctggt tctactgaaa tcacaaatag agctttttta tttcgaatat cattgtaaac
     2161 catgacctta ctattcagtt caacaatttg tgtcatgata ccaagatatg ctctttttac
     2221 ttttcctgtt tgaatcaact caactgcaat atctttcgca gtattgatcc caatggcaaa
     2281 gcaaagtcct tgtgcgccca tgattgtggc agtgtttaca ccaatcacat tcccatcagc
     2341 atcaatcaat ggtccccctg agtttccagg atttaaggga gcatctgttt gtatgatatt
     2401 atcaatcaaa cgtcccgatt gagtccgtag ggttcttcca tttgcgctca cgattccgtg
     2461 agtgactgta tgttgaaatc catgtgggtt tccgattgca ataacgagtt gaccaatctc
     2521 taaatctttt gaatctccga gttttacagt ctggtattca gttgcgaatg tttttaagac
     2581 agctaagtct gagtctggat cttctccaat taaaatcgct tcatattggg aactatcata
     2641 aagagtagtt ctgaatttac ttcccccatg caccacatga ctatttgtaa ataaatatcc
     2701 atctgttgaa aataaaaacc ccgatcctga tccagatgga gtttcttttc cgttgattgt
     2761 tttatatgta tcaattttta caactgaaga ctttgctttg ttgacagcat taataattat
     2821 ttttgaaaat tcatccattg tctaatcctt aaatctcacc aaagatgaat ggaaccatcg
     2881 tggttaaaaa actttccgct atcttccatt tttaatttct gcgaaaactc cagaatactt
     2941 ttagctgatt cttctggttc aagacttgca ctctttccgc ccatatctgt tttcatccaa
     3001 cctggattta cggctaacgt caaaattcca tatttgccta gatcatttgc taatacttta
     3061 ttgaacatat ttaaggcagc tttgcttgca gagtaggaat aatttccttt ttgtggtatg
     3121 gataaggatg cgaaccaaga agaaatattt acaatgagtc ctctttgact cttagataat
     3181 aagtctaaaa attcttgtgc catgatgagt ggtgccacag cgttgaggtg aaacatattt
     3241 aagattggtt ctgcttgaaa tttccccaat tgtaaatgaa tggaggggtc gactccacta
     3301 cttttagagt tcattcctgc attatttacc aatatatcta gtgaaccaaa atgctgtttg
     3361 gcgtattcaa caacttgttt gatgctattt atatttgttg gatcgaggtt aattatgtgt
     3421 aacctctcag taaactcaga ccttaattgt ttcaaatcct ccgctttttc aggatttctg
     3481 catccggcta gaacggtgta tccattatcc aaacttactt tgacgaatgc gaaaccaaga
     3541 cctctgtttg caccagtaat tagtattttc ttgctcattt ttaaatcctt agtgtaataa
     3601 atttagttac atattgtata tgacaggttt taaaatactt tgttttcaat tattttgctc
     3661 tttttaaaat ttcttgcgta atctgatgaa ttgttcgatg ggaaagttct gaaattacaa
     3721 tctgttctgt ctctgtaaaa acatctgtaa gaataggctg aatgtttctt ccacagatgc
     3781 atttggtatt tggcttgctt gcatgcatcg cgaaaagcct ttcattgaaa actcgataga
     3841 catctttcaa tgttaatttc tccgaatcca cagcaagtgt tgctcctccg ccttgcccca
     3901 tctgaatatg aataaatcca gctttcttta gaattcctaa tatcttacga attacaactg
     3961 gatttgtatt cacactcaca gcaagttctt ccgaggtgcg agattgccct tggttcaaat
     4021 ctaacaatga tagtatatgg attgctactg taaatcgact agtcatatta tcttaattgt
     4081 aacatctaac gttacaatta cagaaattga attttcgtaa agatttttct ttattcaaag
     4141 caaaaaagtt tgactattta gtttaggtta caaaaaatca aaactcatgc caacattcaa
     4201 tagaggtaaa tttaagagga aaactagctg aagaatgaag aataaaatta actttaaaat
     4261 ttttttttac attatgacat tcgtagcaat ctcgggtaat gtgttcgcac aagacgccaa
     4321 agatgcagaa aaaaatccta cacaaacaaa tcaaagagaa ccagtaaaag gactgccttt
     4381 tgaaatcagt gaacaaaaga gactttcaga agcggacgta tcaaagaaga aagagggttg
     4441 gtttccaacc ggagtaggag gaccttttag tgatccaaac aacggtgcgg gtgtcggagg
     4501 tagaattttt ctttttaata atggaaaaaa aacagatcca ttttttgaat acactcccta
     4561 tcgtcataga attttcttaa atttgtccaa cacaactcgt aatgctcaat accatcaatt
     4621 ggattgggat gctccgtata tttttgatac aaaatggcgt gctcgcgcaa atgcaattta
     4681 tgatagaaac ccgaataact tattttttgg tttaggtgaa tctacaatga aaggtttgag
     4741 ctatcaccca agaaatgatt attctcaacc tctcgtcaca aatggaactt ttccagagat
     4801 gcaagaagca caagagttta gaagaccgcc taacgcaggt gaaccacaat atctctctga
     4861 caatttttac aattctgcat tattagctaa taatcctgtt ccaggaatga gaaatcaata
     4921 cgtaactgat aaaaaataca accgctatga tttggaagtt ccccaagtta atttgagtgc
     4981 tgaaaattct ttttttggtg gtgtgatgcg tttggttgtt gggactaagc tttccaataa
     5041 tatcgttaga cgttatgatg gaagtattca gaaagcgaat gattcatttt ttagagatac
     5101 accgcttgaa taccaacata ctgttggact aggtgggact ccatttgaat taaatggtat
     5161 tagcgcaaac caaggaaata ttccaactgt aaacggtaaa acaaagataa ccgaggaata
     5221 tgaaagaggt agaataaagg gaattaacgg tggttatgtt aatatcttac gtttcgctct
     5281 tgtttatgat accagagatt ttgagcctga tccaagtaga ggtatatttg ctgaattaac
     5341 tcatgaaaga tcctcaaaag caattggatc aaattttgaa tttaatagaa cgttaggctc
     5401 tgttcgtttc ttctatagcc ccttcccaaa agtatttaaa aaattagtat tggcaggtcg
     5461 tggggttttc gttcacaact caggaaatct tcctttcttc gaatatagaa atatgtggag
     5521 cactgaagga ggtttatctg gtttaggagg tagaacaact cttagaggtt acattcaaga
     5581 tagatttgtt ggaaccaata tgggttttgc aaatttagaa ttgagatgga gatttttcga
     5641 aattcctggt tttactttta acatagcacc tttatttgat cttggtcgag tttgggatac
     5701 tcttggtagt gttaaagcaa atgcttggga tggatataaa tattcctatg gtttaggtct
     5761 tcgtattatt tggaatcaat caactgttat ctatgcagaa tgggcaagat caagagaaac
     5821 tagaacatct ggctggcctg ctgatgcacc tttttcaaat acaaacttct acttcaactt
     5881 tgggcatatt ttttaaggag tgttctatga aaaaattaat gcaatattcg tttttatcaa
     5941 tttgtttaat aattttaatt agctgtgaag ctaaaccgga aaaaagttat tttggtgttc
     6001 cagaggaaga tggaaaacaa attctagcgg ggctcatctt gggcaatggc tctattgaca
     6061 atgcaacagg aactgttgtt gatcctaaga caggtttgga atggaaaaaa tgcacacaag
     6121 ggcaagtttt tcgttctacc aataatgact gtcaagggag tagagcaacc tctacactta
     6181 caactccaat tgatcaagga agatatggag caacctatct agcatactgt aacattgaag
     6241 gaaatgattg caatacatcc tcccttccaa tggcactgaa atcaaataca gttacaggtg
     6301 tatttagtga agcatttcta tcttgcaata cagacagaac aaatggatat actaactggc
     6361 gtgtcccttc ttatccggag ttacaagctt tagcatctct tggaaaagta gttttaacaa
     6421 atcgttttcc aaatacacca gatgattatt tttggtcttc ttggagtaat gaacaagaca
     6481 cttcgggaaa gactgctaga gcagtatcct tttcccttga gacgataggc aaagaccaaa
     6541 acatttccaa aactacacga ttctatgttc gttgtgtccg aaatataaac gggcaatagt
     6601 ttttttggtc tgtttaaaaa ctcaactcac tagactagtt ttaaaaaaac aaattagtcg
     6661 tttttaaaaa acgcagagaa atcaaaagtt tctctgcgaa taattttcca caaaagataa
     6721 aaaacttaga tcttgatcct attaactaac gcttaaacca atctctttag aaataacttt
     6781 aattctatat ttaagtttct cggttccaag taaattaaac aagacaggta attctagacc
     6841 atgtgcttta ccggtagttg ctactcgaat cggcatgtat aagttttttc ctttttcacc
     6901 tgtttctata cctgttttat cgattaacgc tttatattca tctgaacttt tgggctcacc
     6961 atttcctaat aatttaaaaa atgtttgaat cactttgtct gcgtttcctg aggtagctat
     7021 cgttcttgct tcctcatttt cgattacaat gtcattatta aagaattcag agatataatc
     7081 aggcgcttgc gataaacgat ctaaataaac tcggatagac tctaaaatcg attgtaatgt
     7141 tttattctca gcctgtttta tatcatcagg tattgaattg ttatccttga taaagggtaa
     7201 aatctctcct gataatttaa caatatccga atctctaata tacttatttg agagccaatt
     7261 tagtttagat tttggattga gttgatcagc cagtgcggat aacggtagtg aattgaagtc
     7321 taatttttct ttatcttctt ctttgacttt tttaaataca tcaaagagag aaggagattt
     7381 agaacatctc tccacatcaa ataatttttc taactcacct tttggtaaat attcctggcc
     7441 actttcagat gtccaaccta gaagtgccat atagtttcgg aagcattcag cagtatatcc
     7501 taagtctcta aatgcgagaa tcgatgtagc ccctgctcgt ttagataatt ttttcccatc
     7561 cgcacctacg atttccgaag catgcgcata cttaggtaat ggaaagccaa aagcttcata
     7621 tattaagatc tgccgtggtg tattggaaag atgtcctact ccacggataa catgtgttat
     7681 ctccattaaa tggtcatcaa ctacaactgc ataattatag gcaggaaaac catcggactt
     7741 tacgattata aagtccccga ttaactttgt atcaaattta actttacctt gaattatatc
     7801 atctacgatt aaattttttg ctggtgttct gaaacgaacg gagaaaggaa tcttctgatc
     7861 taatttttct tggatttcct tttctgtcat actagaacaa gtcccatcgt aaatattggg
     7921 aattcccatt gcttcagatt gttttttctt tgattctaaa agttctgttg tgcaaaaaca
     7981 tctatatgcg tggttattct gaattagccg ctctgtatat tctttataga tatttaatct
     8041 ttcagattgt ttatacggac catgaggacc tccaacaccc ggaccttcat cccaattgag
     8101 tcccaaccat tccaaagact ctaaaataat tttcatagaa gagtcagttg atctatcttg
     8161 gtcagtatct tcaattcgaa ctaaaaactt accgcctgtt gcttttgcat ataaataatt
     8221 aaatagagcg gttcttgcac cgcccacatg taaaaatccg ctcggtgatg gagcgaatct
     8281 agttctaacg ttttgattca tctgtcatgc tctctttctt tttataaata atgattggta
     8341 gtttgatttt gtataaactt aattgttgat tctttcctga ggaaattaaa ctctcatttt
     8401 gtgcatctaa tatttccatt ggataatagt aaagagtata atctttacta ttatagagtt
     8461 tgatagcaga aggaatatct ttgaatattt ttttataact ataatcttcg aaggcataga
     8521 ttcgtgaata ctccaaggta tttttatttg gagaaaactc tgtatgtgga taattgcgag
     8581 caaccgttcc cgaaggataa tgcttccaac ggattgcctc ttgaccatct ttcaaatgaa
     8641 cctcttccaa tttccataaa ccctcatctg gtgtttgtga gtaggtaact ttctcatgga
     8701 ataattgttg agcatctgtt ttgctactta taaatttatc tattgcagaa agaagatctg
     8761 tgctttgagc atctcgtata gaaaaatttg tatactcaac ttctaaatca ttcgaatcta
     8821 tttttctttg tccttctccg tatttaactt tttcatatat attatacttg ggaggtgctt
     8881 tatcgaaaaa aaggcgtgtt acatcttctt tgaatttatt tgtatacgca taaaaagaaa
     8941 tgtcgatttc tcgcacgctc agcttcccat tttcattttg tctgattact agatatttat
     9001 agtgtgaact ctgtttttca aaaaaactat ttgtgtttag aacttttgca ttttggtagt
     9061 agtcgccaat aaatccttct aaaaattttc tattttctag aaggttattt ccaattgaac
     9121 tgcatgcgga ttgaaaaatt ataaaaaaaa gtaaaaaaaa gtttgctact cgattatgta
     9181 tcatttgagt caataaaatc aggggttttt gcaaattttt aaaaatgaaa aagaggcct
//
LOCUS       DAOQOS010000177         9163 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_4066_length_9163_cov_5.622090, whole genome shotgun sequence.
ACCESSION   DAOQOS010000177 DAOQOS010000000
VERSION     DAOQOS010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 9163)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9163)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9163
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4066_length_9163_cov_5.622090"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..320)
                     /locus_tag="PLF66_23855"
     CDS             complement(<1..320)
                     /locus_tag="PLF66_23855"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002772419.1"
                     /note="catalyzes the formation of L-tryptophan from indole
                     and L-serine; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal-phosphate dependent enzyme"
                     /protein_id="HMZ67145.1"
                     /translation="MKTKIILREKEIPTHWYNIQADMPNPILPPLHPGTKQPVGPEDL
                     APIFPMELIKQEVTQERLVEIPEEVRDIYKIWRPTPLFRAHRLEKFLDTPAKIYYKYE
                     GVSPA"
     gene            complement(647..2278)
                     /locus_tag="PLF66_23860"
     CDS             complement(647..2278)
                     /locus_tag="PLF66_23860"
                     /inference="COORDINATES: protein motif:HMM:NF017106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P83/100 family protein"
                     /protein_id="HMZ67146.1"
                     /translation="MNSFVKFFLIYTLLTSSFIYSQDKAKLGESEIRDSEKIRFTNRS
                     NARAAENVKRQNDSIGKKLSEMIESEPTKTHDYKGISVRRVFADKDGQYGGDIISLEP
                     ESTFGHINSLYRILTSYISNSFGYSEDRADIIALYVLYYNAMHRNQKSYFKSKYTDEL
                     LDILRPSSTGIGKTYKEWPGKTQIVIPLEGNILKENEIDVQLDELGNEVNKIIDEKKN
                     GPEEKRKFAEVIKEKIQEEKKLIQEKKEELKLKEEKIALLEKPQEPKTVVEPKKEPVV
                     EKKAEIKQDPPKEVVKEMVREPVKNTPVEPAKTVAQPIKETAPVKTETAEKPKEVKQA
                     PPVDTEKLKKELKEAKQELAKKTEAENKKKEFSPNVIDGKIVFLKVVKYDTAEGHYNS
                     ELHLIDPEKDNAVSKSDFNKICSRQFKEFGGNLVVVGFKDGHKDEHQLYLISKKDLKE
                     VGKSTTNVFSRTVIEVMGDELYAFEFEKGNYYISKYDKSLRRIMKSDRDINPDSNLTF
                     YGKNIYVTGKAETGNAVDIRIFNKDDLKFIAKIQP"
     gene            2426..3520
                     /gene="serC"
                     /locus_tag="PLF66_23865"
     CDS             2426..3520
                     /gene="serC"
                     /locus_tag="PLF66_23865"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002104563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HMZ67147.1"
                     /translation="MNQIKNRVYNFNAGPAMLPIPVMEQAQREFLDYMGTGMSIMEMS
                     HRGSHFEEILISAERTLRELLSIPENYSVIFYPGGATLQFSAIPLNLLKKGEAADFSL
                     TGVWANKAEQEAKKLGFQTNIIYDDKQNNYTMVPNLTDANISSNAKYLHITSNNTIYG
                     TRYKNLPKIKKVPVVADMTSDLLSRKINVSDFGIIFAGAQKNIGPSGLTIVIARNDLI
                     ESAKDPMPVLLNYSVMAKNKSIYNTPPTYSIYIARLVFDWCKKMGGVEKIEEINEEKA
                     KILYNYLDKSRLYTCPVKGDHRSSMNVVFHLKDKTLEKEFIFSSEKRGIHGLPGHRDA
                     GGFRASIYNAMPMEGVQTLIEFLTEFENKH"
     gene            3617..4342
                     /locus_tag="PLF66_23870"
     CDS             3617..4342
                     /locus_tag="PLF66_23870"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HMZ67148.1"
                     /translation="MKKKYLLTILMVIVSLFSCSKPKEKDDPKIKGDLIFVIGKVEIS
                     NQNGSKEATKNMAVLEGDTIRTGEKSVAGVQFENETASIEIQENAEFTIQKFNKQEQQ
                     LVSTKGNFWIKAKKANKDTNFNVVLPTAVAGIRGTSFYSFEIKEERIHGVCHCQGAVD
                     FQDKVTNYKGSHDTDYGVLTRDGKTITMTPEDFKSVGVNFDKFEHKHSRIDNSPLGKK
                     SSITDEQFANLLKIAKKKFAELK"
     gene            complement(4525..5187)
                     /locus_tag="PLF66_23875"
     CDS             complement(4525..5187)
                     /locus_tag="PLF66_23875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67149.1"
                     /translation="MLKFFLVLFFASFSIVISQANTSNSTSEQKAPHNTLIMDDLIPS
                     GEYLNNLNKYLVKMEKLISERVNQLESLHTRISVIDKNLKDKLSIVEEVPNITAEGHT
                     QIVSSREVEFSFQSDKTKQVKIISKKMNMTNDLYSLVKTITFNPEKIDSLKIKVDQFS
                     SKVESKTEIDEYNDLPLEVKIEALKAIDKILIHTINNFDAVIQKNRVNKVTNGKHNFK
                     DL"
     gene            complement(5454..5693)
                     /locus_tag="PLF66_23880"
     CDS             complement(5454..5693)
                     /locus_tag="PLF66_23880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67150.1"
                     /translation="MFKKRLILSFIFVNSLLLLFSILILNDSSFESFPTEEQEEKKSI
                     VFGLFFFVFFIIEIFLIFIYSKLKSQKLSSKRLPI"
     gene            complement(5832..6083)
                     /locus_tag="PLF66_23885"
     CDS             complement(5832..6083)
                     /locus_tag="PLF66_23885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016545139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2164 domain-containing protein"
                     /protein_id="HMZ67151.1"
                     /translation="MDIELKKNDKDAILASIQKYFREELEIEIGEMQAGFVLKYFLNE
                     IGPFVYNKAIQDAEAFMSDKILDLSAVCYEKGLTYWNKK"
     gene            complement(6341..6571)
                     /gene="vapB"
                     /locus_tag="PLF66_23890"
     CDS             complement(6341..6571)
                     /gene="vapB"
                     /locus_tag="PLF66_23890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004468228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HMZ67152.1"
                     /translation="MQRAKLFVNGRSQAVLLPKEFQFKGNDILIQKIGDAVILLPNNK
                     SWETFLPRLNHFSDDFMKEGRKQNAHQKREKF"
     gene            complement(6765..7073)
                     /locus_tag="PLF66_23895"
     CDS             complement(6765..7073)
                     /locus_tag="PLF66_23895"
                     /inference="COORDINATES: protein motif:HMM:NF016876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HMZ67153.1"
                     /translation="MEEKFKLEKEAREDLAEAFLWHESQKENLGDEFVSNVFEKNNFV
                     SERPYSFSIYHDEYRKAPVKKFPYYISYKIKTVFISIVGIWHTSRDPAKIIKRIDKIK
                     "
     gene            complement(7039..7347)
                     /locus_tag="PLF66_23900"
     CDS             complement(7039..7347)
                     /locus_tag="PLF66_23900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67154.1"
                     /translation="MRAIETLINVKENGEIFLHFPQELKIGIHKIVIVLEDEIVNVEN
                     KIEKEYPISLEIDNAFKEELDKRSERMKVDPHPGYSWDETISYLEEKVGRKIQIRKGS
                     "
     gene            complement(7766..8080)
                     /locus_tag="PLF66_23905"
     CDS             complement(7766..8080)
                     /locus_tag="PLF66_23905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002975343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HMZ67155.1"
                     /translation="MLSIVINLKRYSETALRRKLKDKKFKEYFDAEKEYLNLSIQIAK
                     YREAKGISQKDLAVKAKITQQQLSKIENGINCNITTFLKVCSALDIKIDFQKKNKRAV
                     AG"
     gene            complement(8074..8214)
                     /locus_tag="PLF66_23910"
     CDS             complement(8074..8214)
                     /locus_tag="PLF66_23910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67156.1"
                     /translation="MARYKEIEYKQSLLLAVNYSEQIIEGTFEHALCYLFDEGKIDAT
                     QC"
     gene            complement(8307..8624)
                     /locus_tag="PLF66_23915"
     CDS             complement(8307..8624)
                     /locus_tag="PLF66_23915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004787713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HMZ67157.1"
                     /translation="MEKKGKLTNFITHAEKTIPAWRVNRAKQNARKEILKMKLGDLRK
                     ELGVKQIDIDGFSQSAISRLESRTDFKISTLIDYISQLDLELEITVRPRNSKKKEFVL
                     VKA"
     gene            complement(8635..8970)
                     /locus_tag="PLF66_23920"
     CDS             complement(8635..8970)
                     /locus_tag="PLF66_23920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HMZ67158.1"
                     /translation="MWAVIQTSEFKEWYDELDEDAREDILSSIIILQNTGPILGRPKV
                     DTLKGSKIKNLKELRVQSNGRPFRVFFLFDSKKKIVLLTGGNKQNDKSFYKRKILESE
                     KIYKEYFSS"
BASE COUNT         2941 a         1553 c         1373 g         3296 t
ORIGIN      
        1 gcgggactaa cgccttcata tttataataa atcttagctg gagtatctaa aaatttttct
       61 aagcggtgtg ctctaaaaag tggagttggt ctccaaatct tatagatgtc tctcacctct
      121 tctggaattt cgaccaatct ctcttgtgta acttcttgct tgattaattc cattggaaaa
      181 ataggagcaa ggtcttctgg accgactggt tgtttcgttc cagggtggag aggtggtaaa
      241 attggattcg gcatatctgc ctgaatatta taccagtgtg ttggtatctc tttttctcgt
      301 agtatgattt tagttttcat aattcctaaa ctagttttct tattaattat gaaaagaaaa
      361 aaaaggaatc gagtaaaaac caatttgatt tagattttga aacaattcct cttcattaag
      421 actgcgcctc aaatgcacga gacgcataac gcttttcgat taatgtgaat ccgatttatt
      481 ttttttttac caccgataac ggaccgatga agacgcgatg gataattttg aattatcgta
      541 tcttatatcg gtgataatcg tctctccacc caaaatccat taccgctcat tcgcggtaat
      601 aggtggtaat cttttaattc ttctattaca tcgaactgac tttttattaa ggttgaattt
      661 tagctataaa tttcaaatcg tctttgttga agattcgaat atccactgca tttcctgttt
      721 ctgcttttcc agttacatag atattttttc cataaaaagt tagattggaa tcaggattga
      781 tatctctatc tgacttcata attcttctaa gagatttgtc atatttagaa atatagtaat
      841 ttcctttttc aaattcaaaa gcatagagtt catcacccat tacttcaatt acagttctag
      901 aaaagacgtt agttgttgac ttaccaactt cttttaaatc ttttttgctg attaaataga
      961 gctgatgttc gtctttatga ccatctttaa aacctactac tacaaggttt ccaccaaatt
     1021 ctttgaactg tcgactgcaa attttattaa aatcgctttt agaaactgca ttgtcttttt
     1081 ctggatcgat tagatgcaat tcactattat aatgtccttc ggctgtatca tactttacta
     1141 cttttaagaa tacaatttta ccatcaatga cgttaggaga aaactctttc tttttatttt
     1201 ctgcttctgt ctttttagca agttcttgct ttgcttcttt taattctttt tttagttttt
     1261 ctgtatcaac cggaggagct tgttttacct cttttggttt ttcagcagtt tctgttttaa
     1321 caggagcggt ttcttttatg ggttgtgcga ctgtcttagc tggttcaacg ggagtatttt
     1381 ttacaggctc tctcaccatc tcttttacaa cttcttttgg aggatcttgt tttatttctg
     1441 ctttcttttc tacaacaggc tcttttttag gttccacaac cgtttttggt tcttgtggct
     1501 tttcgagaag agcaatcttt tcttctttga gttttaattc ttcttttttc tcttggatta
     1561 atttcttttc ttcttgaatt ttctctttaa tgacttctgc aaatttcctt ttttcttccg
     1621 gtccattttt tttctcatcg atgattttgt ttacttcgtt tccgagttca tctagttgca
     1681 catcgatttc attttctttt aaaatatttc cctctaaagg aattacaatt tgcgttttac
     1741 ctggccattc tttgtatgtt ttaccaatcc ctgtgctact aggtcttaga atatctagga
     1801 gttcatcagt gtattttgat ttgaaataac ttttttggtt tcgatgcatc gcattgtaat
     1861 aaagaacata gagagcgatg atatcagctc tgtcttctga atacccaaaa gaattggaaa
     1921 tataagaagt taaaattcgg tataaggaat tgatatgacc aaatgtagat tctggttcta
     1981 aagaaataat gtctccacca tattgaccat ctttatctgc aaagactcgg cgaaccgaaa
     2041 tgcctttata gtcatgtgtc tttgttggtt cactttcgat catctcggaa agttttttac
     2101 caatggaatc attctgcctt ttcacatttt ctgctgctct tgcattggat ctgttggtaa
     2161 aacgaatttt ttctgagtct cggatttcac tttcccccag ctttgcttta tcttgggaat
     2221 aaataaaact agaagttaat agagtataga ttaaaaaaaa ttttacaaag gaattcataa
     2281 attacacttc ctataagagt taaataaggc aatcacaaaa gaaattcgat tcccactata
     2341 agaatgtcgg actgaaaaag gcaaagattg attttatggt aaataaatta tttgatttca
     2401 aaagatagaa aaaaatcata tatctatgaa tcaaattaag aacagagtat acaattttaa
     2461 tgctggacct gctatgcttc caatcccagt gatggagcaa gcgcagagag aatttttaga
     2521 ttatatggga acaggaatgt ccattatgga aatgagtcat agaggctccc attttgagga
     2581 aattttaatc agcgcagaac gcacattaag agaactactc tcgattccag aaaactattc
     2641 tgttattttt tatccaggag gggcgactct tcagttttca gcaattcctt taaaccttct
     2701 aaaaaaagga gaagcagccg atttttctct aacaggcgtt tgggcaaata aggcagagca
     2761 agaagcaaag aagctaggtt ttcagaccaa tatcatctat gatgacaagc aaaataatta
     2821 tacaatggtt ccaaacctaa cggatgcaaa catcagttcc aatgcgaagt atttgcatat
     2881 cacatccaat aacacaatct atggaacccg ttataaaaac ttaccaaaga taaaaaaagt
     2941 tcctgttgta gcagatatga caagtgatct actttctaga aaaatcaatg taagtgactt
     3001 tggaattatt tttgccggtg cgcaaaaaaa tataggacct tcgggcttaa cgattgtaat
     3061 tgctcgcaat gatttgattg aaagtgcaaa agacccaatg cctgttttac tcaattactc
     3121 tgtgatggca aaaaataaat ctatctacaa cactccccca acctattcca tttatattgc
     3181 aagactcgtt tttgattggt gcaaaaaaat ggggggtgtg gaaaaaatag aagaaatcaa
     3241 tgaagaaaaa gcaaagattc tctacaatta tctagacaaa tccagactct atacctgtcc
     3301 agtaaaggga gatcatagat cttctatgaa cgttgttttc catctcaaag acaaaacctt
     3361 agaaaaagag tttatctttt cgagtgaaaa acggggtata cacggattac ccggtcatag
     3421 agatgcaggt ggatttagag catctatcta caatgcgatg ccaatggaag gggttcagac
     3481 tttgattgag ttcctaaccg aattcgaaaa caagcactga gactaatgcg aatttgccgc
     3541 aaaattttat tcaaaacctt aaaaaatgaa ttgtaacaat ttcagtaaca tttaaaatga
     3601 aaaatacgag gtatatatga aaaaaaagta tttattaaca attcttatgg ttatagtttc
     3661 tctatttagt tgcagcaagc cgaaagaaaa agatgatcca aaaatcaagg gagatttaat
     3721 ttttgtaatt gggaaagtag aaatttcaaa tcaaaacgga tctaaagagg ctacgaaaaa
     3781 tatggctgtt ttagaaggag acacgattcg aaccggagaa aaatcagttg ctggcgttca
     3841 gtttgaaaat gaaacagcga gtattgaaat ccaggaaaat gcagaattca caattcagaa
     3901 attcaacaaa caagaacaac aacttgtatc tacaaaagga aatttttgga tcaaggcaaa
     3961 gaaagctaac aaagatacaa acttcaatgt ggttttacca acagctgtag ctggaatcag
     4021 aggaactagt ttctattctt ttgaaatcaa ggaagagcgg atacatggtg tttgccactg
     4081 tcaaggagca gttgactttc aagacaaagt gacaaattac aaaggaagcc atgatacaga
     4141 ttatggagtc ttgacaagag atggaaaaac tatcacgatg actccagaag attttaaatc
     4201 agttggagtc aactttgata aatttgaaca taaacatagt agaattgaca atagtcctct
     4261 tggaaagaaa agctctataa cagatgagca atttgcgaat ctattaaaaa tcgcaaaaaa
     4321 gaaatttgca gaattaaaat aatctgaatt cgatctaaga atttttcgcc aacgacttgc
     4381 actgagctat gtcgaatgcg tgcacacaga taactgaacg ataggataac ttttaaatat
     4441 cgtatctttt atctgtgttt atcgtcccta tcggtgataa tctttttaat tttcccttac
     4501 atggaactga cgttaaatta aatattataa atctttgaaa ttatgctttc cgtttgtaac
     4561 tttattaact ctgttttttt gaatgactgc atcgaagtta ttgatcgtat gaattaaaat
     4621 tttatcaatt gccttgagcg cttcaatctt tacctcaagt ggtaaatcat tgtattcatc
     4681 aatttctgtt ttactttcta ctttagaact aaattgatct actttgattt ttaaactgtc
     4741 tattttctct ggattgaatg taatcgtttt aacaagagag taaagatcat tggtcatatt
     4801 cattttttta gaaatgattt taacttgctt tgttttatca gattgaaagg aaaactcaac
     4861 ttctcgagag gagacaattt gtgtatgtcc ttctgcggta atattaggaa cttcttccac
     4921 tatagagagt ttgtctttta gatttttatc aatcacggaa attctagtat gaaggctttc
     4981 taattgattt actctctcag aaatgagttt ttccattttg actaaatatt tatttaaatt
     5041 gtttaaatat tcaccacttg gaattagatc atccatgata agagtgttat ggggagcttt
     5101 ttgctcgctt gtagaattgg aagtattcgc ttgagagata acaatactaa aagaagcaaa
     5161 aaaaagaact aaaaaaaatt ttaacataaa ttaaacctca tttcttaaga caaataaaac
     5221 tatgtctttt tacaattagc gaaaaattct ttgctagttt cctttttagg caagcaattt
     5281 atttccaaat tgaaaaaatt tttcctaaaa tagattgcat tttatttcta ttttaatctc
     5341 caaatagaag agaggtaaat tacaaaatgc ggcaaatggt ggtttgccac tgagaatcaa
     5401 atcttttatt tcaaaaaagg gaaccaattt tgaggcttca gaaagaatga aactcaaata
     5461 ggtaaacgtt tagaagatag tttctgagat tttaatttag aatatataaa aattaaaaat
     5521 atttcgatta taaaaaatac aaaaaagaat aaaccaaaaa caatcgattt cttttcttct
     5581 tgctcttcgg ttgggaagga ttcaaaactg ctatcattta aaattaaaat tgaaaataga
     5641 agaagtaaag aattcacaaa aataaaactt agtattaatc tctttttaaa catttctttt
     5701 tcctttttca tcttttttca aattcattgt attttaagtg agtcgatcga ctgtttaaaa
     5761 agtcaaataa tttaatctac ttcctttcta gaaaagaatc acgaaaaata aaaaaagaag
     5821 gatagatcta gttatttctt attccaatag gttaaacctt tttcgtaaca gacagctgat
     5881 agatccaata tcttatcact cataaacgct tccgcatcct ggatcgcttt attgtaaaca
     5941 aagggaccaa tttcatttaa aaaatatttt aatacaaatc ctgcttgcat ttctccaatt
     6001 tctatttcca attcttcccg aaaatacttt tgaatagatg caagaattgc gtctttgtcg
     6061 tttttcttta gttcaatatc cattttgtca ttttctattt tccctttttg attggaataa
     6121 gaaacaaatc tatgaatctg gaaactttgt tattctaaca tgaattcgat aaaagaattt
     6181 tcaccaccaa taaacaccga ggacaccgat ttcaccacat cgaacacaga tttttatagt
     6241 gctcagcaaa ctctaagtaa ttttgttttt aaagttttta atacttcgat tggttttttc
     6301 tttaaaatgt aaatacagat attcgtagca agtaaataca ttaaaatttt tctctttttt
     6361 gatgagcatt ttgcttgcga ccctctttca taaaatcatc cgaaaaatga ttcaatctag
     6421 gaaggaaagt ttcccatgat ttattattag gaagcaagat aacagcatcc ccaatttttt
     6481 gaatcaaaat atcgttacct ttaaattgaa attcctttgg taagagaaca gcctgacttc
     6541 taccgtttac aaataattta gccctttgca tagaacatat cctccaacaa aatatatatc
     6601 aagatatata ttttgtctta taaatttatt caatcgagaa aaggttctga tgaattctgg
     6661 aagatggaag gcagttgatt tgcgttagtt aatcaaataa actaaaaatt ctaattaaac
     6721 aaccagatgg ctaggagtaa ttgtataaag ctattctaaa acaattactt tattttatca
     6781 attctcttaa ttatctttgc ggggtctcta ctcgtatgcc atattccaac aatagaaata
     6841 aagacagttt tgattttata ggaaatgtaa taagggaatt ttttgactgg tgcttttcta
     6901 tactcatcat gataaataga aaatgaatag ggacgttcac ttacaaaatt atttttttca
     6961 aatacgttac tcacaaactc atcaccgaga ttttcctttt gtgattcatg ccaaagaaat
     7021 gcttctgcaa gatcttctct agcttccttt tctaatttga atttttcttc caaccttctc
     7081 ctccaaataa gaaatagttt catcccaact gtatccagga tgagggtcta ctttcattct
     7141 ttcacttctc ttatctaatt cttctttgaa tgcattatct atttcgaggg aaatcggata
     7201 ttctttctca attttatttt cgacattaac aatttcgtct tctaatacaa ttacaatttt
     7261 atgtattcct attttgagtt cttggggaaa atgtaaaaaa atttctccat tctcttttac
     7321 attgattaaa gtttcaatgg ctctcataca ataacttcct tttttgtcgt acaggatact
     7381 taaatcagaa caattctgtt ctttaaacga ataactaaat tttcttccag ccgattgcct
     7441 ttgtcaagta gaaagtaacc ttcattcagt taagaaaagg ttaccaaata atttctaact
     7501 ctgccaattt tcccgttttt tcgtttgtta gaataatcaa taatcttttg tcttctgtat
     7561 gagaacaaac aaggcaagta gattctccac gacgataatg ttggaattga atcgtattac
     7621 aatttgggtg tccacatttg caactatcca cgacaaataa atcttccact tgcttcagaa
     7681 aaaaatctga ttcaattggt tcctctaatt cttttagttg actggtttca atatcaatcg
     7741 actcagcttc ggcaaagtat tcaagttagc cggcaactgc cctcttattt tttttttgaa
     7801 aatcgatttt aatgtccaat gcagaacaga cttttaagaa ggtagttata ttacagttta
     7861 ttccattttc aattttcgat agttgctgtt gtgttatttt tgccttaaca gctaaatctt
     7921 tttgggaaat acctttcgcc tctctatact tagctatctg aatcgataaa ttcaaatatt
     7981 ctttttctgc atcaaagtat tctttaaatt tcttatcttt taatttcctc ctcaatgcag
     8041 tttctgaata tcttttcaga tttataacta tacttagcat tgcgttgcgt ctattttacc
     8101 ttcatcaaat aggtaacata acgcatgctc aaacgttccc tctattattt gttctgaata
     8161 atttactgct aataataaac tttgcttgta ttctatctct ttgtatctcg ccataatttt
     8221 catatctatc gatattcaaa ttatgggtac tactttttta ctttttcgac agactcgtta
     8281 ggcgtggctt tggcgcactg ggacaactaa gccttaacta aaacgaattc ttttttctta
     8341 gaatttcttg gtcgaacagt tatttccaat tctaaatcta attgtgaaat gtaatcaata
     8401 agagtagata ttttaaaatc agttctagat tcaagtcttg aaatagcaga ctgactaaaa
     8461 ccatcaatgt caatctgttt aactcctaat tcttttctta aatctcctaa tttcattttt
     8521 aaaatttctt ttcttgcatt ttgtttagct ctattaactc tccatgcagg aatagttttt
     8581 tcggcatgag taataaaatt tgttaacttt cctttctttt ccatattcat tctcctatga
     8641 agaaaaatat tctttataaa tcttctcaga ttctaatatt tttcttttat aaaaactctt
     8701 atcattttgt ttatttccac cagttaataa aactatcttc ttcttagagt caaagagaaa
     8761 gaatacacga aatggtctcc cattactctg aactcttaac tccttcaaat tcttaatttt
     8821 agaaccttta agtgtatcta ctttcggtct tccaagaatc ggtcccgtat tttgaagaat
     8881 tataatagag cttaaaatat cttctctagc atcttcatct aattcatcat accactcttt
     8941 aaactcacta gtctggataa ctgcccacat cttttccaat atgacttata tgtaatatgt
     9001 agtcaaatac attttttatt ctttttttga agtttagcca aaatcacgca taacgttaga
     9061 gcgtatgaga cgtttttgtg aaggcgcgaa ctttgcttcc ctttgcaaag tgaaccccta
     9121 cataaaaaaa ggacacgatt ttaggcttcc aaaaggagac taa
//
LOCUS       DAOQOS010000178         9087 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_4116_length_9087_cov_5.441873, whole genome shotgun sequence.
ACCESSION   DAOQOS010000178 DAOQOS010000000
VERSION     DAOQOS010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 9087)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9087)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9087
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4116_length_9087_cov_5.441873"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..212
                     /locus_tag="PLF66_23925"
     CDS             <1..212
                     /locus_tag="PLF66_23925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67159.1"
                     /translation="NRIQPGFCELFSVLLYDRNVNAGVEISIATQSRISLICLKQEQD
                     RKECDGKSQYWPLPKNFNQGKHRGL"
     gene            458..553
                     /locus_tag="PLF66_23930"
                     /pseudo
     CDS             458..553
                     /locus_tag="PLF66_23930"
                     /inference="COORDINATES: protein motif:HMM:NF016875.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            565..855
                     /locus_tag="PLF66_23935"
     CDS             565..855
                     /locus_tag="PLF66_23935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016759991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HMZ67160.1"
                     /translation="MATNRKPTHPGKVLFYDVLEPLGLSITEAAKYLGVSRKTISELV
                     AGKCSLSAEMAVRISKATNTSPESWLYMQSKLDLWKASRKPIKNITLLPTTV"
     gene            complement(1412..1546)
                     /locus_tag="PLF66_23940"
     CDS             complement(1412..1546)
                     /locus_tag="PLF66_23940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67161.1"
                     /translation="MSFRWANVNLAILPTVLRKQNESNGIQRININNIVEGVSNSILL
                     "
     gene            1751..2431
                     /locus_tag="PLF66_23945"
     CDS             1751..2431
                     /locus_tag="PLF66_23945"
                     /inference="COORDINATES: protein motif:HMM:NF025592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4334 domain-containing protein"
                     /protein_id="HMZ67162.1"
                     /translation="MKLKNGKLILFFPSIVYEILQWVVFPLNYLIGIVCNIQPGRSVW
                     NEKDFKSQKLEGSGRSLADIKNDIVSENDVAYKEADLVLLYDSLPEVSAYNDMIGNTW
                     KGKILRTNASVLDVAEWLLVRPLGLLGLKWGKRFRQQDLGDPLIVRWFNRFYFPLPIW
                     GNVCMIDVRWRDVPTATMIYDRQPWKDYFRLLHKENGKVVLLGVWTHKEIAGGWFTLT
                     LDADAVTN"
     gene            complement(2661..5978)
                     /locus_tag="PLF66_23950"
     CDS             complement(2661..5978)
                     /locus_tag="PLF66_23950"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014803818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HMZ67163.1"
                     /translation="MTNKITESTIEEFAIQLLEKKGYEYIYGPDIAPDSENQNSQFGN
                     RKSFEDVLLLERIQKAVARINPTIPSDALEDAVKQIERISSSELIVNNEMFHRMLTEG
                     VKVNYQKDGSSRGDLVWLIDFNETENNDFLVVNQFTISRRDGFQTHPYNNSHKKRPDI
                     ILFVNGLPLVVIELKNPADQNTTVNSAYKQLQTYKEMIPSLFTYNTFLIVSDGLEARA
                     GTISSAFSRFMTWKTEDGKEEASHLIGQLEILIKGMLGKETLLDLIRHFLVFESSKKE
                     DKATGIITLQTIKKLAAYHQYYAVNRAVESTLRASGFITKSKKLNESLVIHSPELHGL
                     KSNQPQKIGDKKGGVIWHTQGSGKSLSMVFYTGKIVLAMDNPTILVITDRNDLDDQLF
                     DTFAAAKQLLRQEPKQVDNREQLKDLLKVASGGVIFTTIQKFQPEEGNVYETLSERKN
                     IIVIADEAHRTQYGFKAKTIDDKDDKGNVIGKKVVYGFAKYMRDALPNATYLGFTGTP
                     IENTDVNTPAVFGNYVDIYDIAQSIHDGATVRIYYESRLAKISLSEEGKRLVQELDDE
                     LEKDESDDAGNGRDRSLQKDDRSQHAKSKWTQLEALIGSANRIKQVAKDLIAHFEERQ
                     RAMDGKAMIVTMSRRIAAELYNEIISLRPDWHNEDKKKGKIKVVMTSASSDGPNLAKH
                     HTTKEERRALAERMKDSEDELKLVIVRDMWLTGFDVPSMHTLYIDKPMKGHNLMQAIA
                     RVNRVYKDKTGGLIVDYLGIASDLKKALSFYADSGGKGDPAVAQEQAVQVMLEKLEVV
                     SQMFNGFAYEEYFTADTGRKLYLILAAEEHILGIEDGKKRFIDCVTALSQAFSIAIPH
                     DKAMDAKDEVAFFQAIKARLVKFESTGAGRSSEEMETAIRQVIDKALVTDKVIDVFDA
                     AGIKKPDISILSEEFLLEVKNMEHKNLALEVLKKLLNDEIHSRTKRNLIQSRTLIEML
                     ETSIKKYHNKILTAAEVIEELIKLSKEIQNMDKEPKEMGLSEQEYAFYTAIANNDSAR
                     ELMEKEKLRELAVILFQKVKENASIDWTIKESVKSKLKVIVKRILRQFGYPPDMQLLA
                     TETILQQAELIAEELAQ"
     gene            complement(5968..6984)
                     /locus_tag="PLF66_23955"
     CDS             complement(5968..6984)
                     /locus_tag="PLF66_23955"
                     /inference="COORDINATES: protein motif:HMM:NF024706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence RhuM family protein"
                     /protein_id="HMZ67164.1"
                     /translation="MNQSSIIFYTSPDGNIKIDVYIKDETVWLTQESIARLFGVKRPA
                     ITKHLKNIFESGELEEKVVSSILEHTTQHGAIEGKQQTQAVKYYNLDAIIAVGYRVNS
                     YQATQFRIWATKTLKEFIIKGFVLDDERLKQGKHFGKDYFDELLERIREIRASERRFY
                     QKITDIYALSADYDKNSPTTKDFFASVQNKLHWAITGKTAAEIIYDSADATKIYMGLT
                     NWKQAPDGKILKSDVSIAKNYLNKAHMEELNRIISAYLDLAENRAKRQIATTMEEWVK
                     FLHSFLELSNYQILTDKGKVSMLEAKLKAEKEFETFRITQDENYISDFDKEVKRILGE
                     ANDK"
     gene            complement(7186..7734)
                     /locus_tag="PLF66_23960"
     CDS             complement(7186..7734)
                     /locus_tag="PLF66_23960"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HMZ67165.1"
                     /translation="MIEKIGKHVIDKIKDKKVFMDANILLYIFWPMGNPSWVKEYSSF
                     FKDLLKQKIVIVTDRTTLSEFINRALRYEYETHLRENNLDKRSLPFKEFRDQNYGKET
                     VKEIYSITKEKILNQFEYLDKSFSKEEVLEILDKENGDFNDQLIAKFCQENSLFFLTN
                     DGDFKNTGIDILTANPILLNPD"
     gene            complement(7731..8084)
                     /locus_tag="PLF66_23965"
     CDS             complement(7731..8084)
                     /locus_tag="PLF66_23965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002976714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS-like domain-containing protein"
                     /protein_id="HMZ67166.1"
                     /translation="MNTVDISVFSIVGSPICVDAEDGERVYELIHKTLKSNSKANISF
                     LNVDMLTSAFLNTAIGKLYNDFSEDEIKKMVSITNISDVDKSLLKRVVKNAKLYYKDP
                     TWMEKTLSEITGENP"
     gene            complement(8093..8953)
                     /locus_tag="PLF66_23970"
     CDS             complement(8093..8953)
                     /locus_tag="PLF66_23970"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HMZ67167.1"
                     /translation="MKEFILKNISASFESFEQLIELYDSNKKSFFQTIEISFQDWFGA
                     NMSAALGGILDKLSGNLNKIQIKNVPQNIETIIRKNGFLAHYGYTKRIDEHHTTIPYL
                     KLTPKDGKFFNHYILNELIERPELPEMSIPLKKKIAESIYELFANAKMHSETKYIYTC
                     GQFFPKKNYIEFTITDTGIGFKKKINNRFNKNLSATDSIRWAVSSGHTTKVGTSGGIG
                     LSILKEFVEKNDGKIQIISDTGFYQFGSKREIVKNFSGAFPGSVVTVQFRTDDTQSYS
                     LIDEDSEDIF"
BASE COUNT         2651 a         1703 c         1470 g         3263 t
ORIGIN      
        1 cgaatagaat acagcccgga ttctgtgagt tatttagtgt tttattgtat gatcggaatg
       61 ttaatgccgg tgttgaaatt tcaattgcta cgcaatcaag gatttctctt atttgcctaa
      121 aacaggaaca agacagaaaa gagtgcgatg gcaaatctca gtattggcct ctacctaaaa
      181 atttcaatca gggtaaacat agaggactat aaaaatgtca attacaaacg attaagagga
      241 taatttcaat ttgccgatgg aatgataaaa cgtagcgtca aggatgcaaa ttaaccgatg
      301 ggcgcataaa gtttgatacg aatagtaagc aagtgtccta aatatttata gtttaaaaaa
      361 aggagaatag atttgttttc agaattaaaa cagttatttt ttattttagt agtaatagtt
      421 actactttct tttcttgcct cggctggaaa gcgaaaaaaa gaatgggcag taaccgtctc
      481 ggggaattgg acaattgttt ttgaattctc aggcgaaaat gcggctaacg tagattatct
      541 tgattatcat taaaggagaa atatatggca acgaacagaa aaccaacgca cccaggaaaa
      601 gtattatttt acgacgttct agaaccttta gggctatcca taacagaggc tgctaaatat
      661 ctaggtgttt caagaaagac aatatctgaa ttagtggcag gcaaatgttc cttatccgct
      721 gaaatggcag ttcgaatttc caaggcaacc aatacatctc cagaaagttg gctctatatg
      781 caatcgaaac tagacctttg gaaagcaagc agaaagccaa ttaaaaatat tactcttttg
      841 ccaacaactg tataaaaaaa tttacttcgc ctatttcgaa aatttctttc tgaaagattc
      901 gtaaccgatt tttgattcaa agatttcata gaaatttaaa ttcgaaacaa taaaacaatc
      961 tcccgttctc cctgtcggct actccttatt agccacacgt attaagaaaa atcagatgat
     1021 cagtctgtat ttactctctt ctaactaaaa gaattaccca aaagaaggat tagaggattg
     1081 cttccatcct atacgcaaaa tagacttctc tctgagtcga gtttttgcca ttctgatttg
     1141 tgaaataaca gaagtaggac ttatagaatt gggaggagtc ggaacaggcg gaaaaacagt
     1201 gaataaaaat gaacttcttt taaatctgcg actctatcct aaagttcaat cctagttttg
     1261 tatttattcc ttaagtttaa aaactttcga cgtttccttg atttctatcg atgttctgat
     1321 tttagcaaat cttcccatct atcctgatga gtttcaagat attcttacaa atcttgttta
     1381 gattttagag gcttttttaa atccattcat tctataataa aatcgaattt gaaactccct
     1441 cgactatatt attgatatta attctctgaa ttccatttga ctcattttgc tttcttagaa
     1501 ccgttggtaa tattgcaaga ttgacgttag cccagcgaaa gctcaatgat ctcaacctaa
     1561 ggattaccac cgattcgcac cgataatcga acgataaaaa cgatggataa ttttggatta
     1621 tctcatctct atccgtgttt atctgtgttt gaactcaatg ttgcttccag agtcgcaaca
     1681 taatgtggta aaaaatctta aaacattgcg ctcaggcgaa tgggtaaatc aactagttca
     1741 ggaggagtaa atgaaattaa aaaatggtaa gctaattctt ttctttccat caattgtata
     1801 tgaaattctt caatgggttg tcttcccgtt gaactatcta attggtattg tgtgcaacat
     1861 ccaaccaggt agatccgttt ggaatgaaaa agacttcaaa tcacaaaaac tagaaggctc
     1921 aggtcgttct ctagctgata taaaaaatga tatcgtttct gaaaatgatg tagcttacaa
     1981 agaagcagat ttagtgcttc tatatgattc tcttcctgaa gtaagcgcct ataacgatat
     2041 gatcggaaat acttggaaag gaaaaattct aagaaccaat gcatctgttc tagacgtagc
     2101 agaatggtta ctcgttcgcc cactcggttt acttggttta aaatggggca aacgttttag
     2161 acaacaagac cttggtgatc cgttaatcgt tagatggttc aatcgttttt atttcccact
     2221 ccctatctgg gggaatgtgt gcatgatcga tgttaggtgg cgtgatgttc caactgcaac
     2281 tatgatctat gacagacaac cttggaaaga ttacttccgc cttctccaca aagaaaatgg
     2341 aaaagttgta ttactcggag tttggactca caaagaaatc gctggtggat ggttcacact
     2401 cacgttagac gctgacgctg ttacgaatta ggtagagcaa cttcgtcagt cgcgctgact
     2461 tatagaaaat tacaaatccc ttttcttatt tccgcttccc agcggtgggg tattcttctt
     2521 actttatttc ttttttatgt aaaaaagaaa taaagtaaca aacaaagaaa aaacccgaaa
     2581 ctgcggcatt ttcgtactaa cacacaggat tctccggttg ggctttttgt atttcagttt
     2641 tttcttttta acgattatat ctactgtgct aattcttctg caataagctc tgcttgttgt
     2701 aatattgttt cagtagcgag aagttgcatg tctggtggat aaccaaattg gcgtagaatt
     2761 cgttttacaa ttactttgag ctttgatttt acactttctt taatcgtcca gtcaatggat
     2821 gcattttctt ttactttctg aaacaagata actgctaact ctctgagctt ttctttttcc
     2881 attagttctc tagcactatc gttattagca atagcggtat agaacgcgta ttcttgctct
     2941 gacaaaccca tttcctttgg ttctttgtcc atgttttgaa tttctttaga gagtttaatt
     3001 aattcttcta ttacttctgc ggcagttaaa attttattat ggtatttctt aatggaagtc
     3061 tcaagcattt cgattaatgt tctactttga attaaattcc tttttgttct tgaatgaatc
     3121 tcatcattta ataatttctt taaaacttct agagctaagt ttttatgctc catgttttta
     3181 acttctagaa gaaactcttc cgacaaaata gatatatccg gtttttttat tccagccgca
     3241 tcaaatacat caatcacttt gtcggttact aacgctttgt ctataacttg acgaattgca
     3301 gtttccattt cttcactgga acgtcctgct ccagtagatt caaacttaac aagccttgcc
     3361 ttaatagctt gaaagaaagc aacttcatct ttcgcatcca ttgctttatc atgtggaatt
     3421 gcaatggaga atgcctgcga caaagcagtc acacaatcga tgaatcgttt ctttccatct
     3481 tcaattccaa gaatatgctc ctctgccgcc aagatgagat agagttttct gccagtatcc
     3541 gctgtgaaat attcttcata agcaaagcca ttaaacattt gcgaaactac ttctaatttt
     3601 tctaacatta cttgcacagc ttgttcttgt gctactgctg gatcgccctt accacctgaa
     3661 tcggcataaa acgaaagagc tttttttaga tcagaggcta ttcctaagta atctacaata
     3721 agtccacccg ttttatcttt atacactcga ttgacgcgag caatagcttg cattaaattg
     3781 tgtcctttca ttggcttatc aatataaagc gtatgcatgg acggaacatc aaaaccagtt
     3841 agccacatat ctcgaacaat tactaatttc aattcatctt ctgaatcttt cattctttcg
     3901 gctaacgccc ttctttcttc tttggttgta tgatgttttg ctaggtttgg tccatcagaa
     3961 gaagctgatg tcatcactac tttgattttg cctttctttt tatcttcatt atgccaatcc
     4021 ggtcgtaagg aaataatttc attgtataat tctgcggcaa ttcgacgtga catggtaaca
     4081 atcattgcct taccatccat agctctttgc ctttcttcaa agtgtgcaat taaatcctta
     4141 gcaacttgtt tgattcgatt cgcactacca attaaagctt ctaattgagt ccatttggat
     4201 tttgcatgtt gggaacggtc atctttttgt aaggaacggt cgcgaccgtt ccctgcgtca
     4261 tcagattcat ccttttctaa ttcatcatct aattcttgga ctaatcgttt tccttcttca
     4321 ctcaggctaa tcttggctaa ccgactttca tagtatattc gaactgttgc tccatcgtga
     4381 atggattgcg caatatcata aatatccaca tagtttccaa aaacggctgg agtatttaca
     4441 tctgtatttt caatgggcgt gccggtaaaa cctagatagg ttgcattcgg caaagcatcc
     4501 ctcatatact ttgcaaagcc atagactacc tttttcccaa ttacatttcc cttatcgtct
     4561 ttatcatcaa ttgtttttgc tttaaatcca tattgggttc tatgtgcttc atcagctatg
     4621 acaattatat ttttacgttc cgataaagtt tcgtaaacgt ttccctcttc tggttggaat
     4681 ttttgtattg tagtgaagat gactccacca gaggctactt tcaataagtc ttttagttgt
     4741 tctctattat caacttgctt aggctcttgt cgtaaaagtt gttttgccgc cgcaaaggta
     4801 tcgaataatt gatcgtctaa gtcatttcta tctgtaatca caagaattgt aggattgtcc
     4861 atggctaata ctattttgcc ggtataaaaa accatagaaa gagatttccc acttccttgg
     4921 gtatgccaaa taacgcctcc ttttttatct ccgatctttt gtggttgatt gctttttaat
     4981 ccatgcaatt caggtgagtg aattacaagt gattcattta gtttttttga tttagtaata
     5041 aacccagaag ctctgagagt agactcaact gctcgattga ctgcatagta ttgatgatac
     5101 gccgccagtt ttttaatcgt ctgtagcgtt ataatgccag tagccttgtc ttccttcttt
     5161 gaagattcaa atactagaaa gtggcggata aggtctaaca gagtttcttt tccaagcatt
     5221 cctttgatta gaatttctaa ttgacctatc aggtgcgacg cttcttcttt tccatcctcc
     5281 gttttccaag tcataaaccg actaaatgca gaagaaatag ttccggctct tgcctctaag
     5341 ccatccgaga caattaaaaa ggtattgtaa gtaaataaag aaggaatcat ttctttataa
     5401 gtctgcaact gtttataagc agaatttacg gtagtgtttt gatccgctgg attttttaat
     5461 tcaataacaa ctaaaggtaa accatttaca aataaaatta tatcaggacg tttcttatga
     5521 gaattattgt aaggatgggt ttgaaaccca tcccgacgtg aaattgtaaa ttgatttaca
     5581 acaagaaaat cattattctc tgtctcatta aaatcaatca accatactaa atcccctcga
     5641 ctgcttccat ccttttgata atttaccttt acaccttccg tgagcattcg atgaaacatc
     5701 tcgttattca caatgagttc cgaagaacta atacgttcaa tttgttttac tgcatcttct
     5761 aacgcatcac tcggaatcgt tggattgatt cgtgcaaccg ctttttgaat tcgttctaat
     5821 agcaatacat cttcaaaaga ttttcgattt ccgaattgcg aattttgatt ttcggaatcc
     5881 ggtgctatat cgggtccata gatatattca taaccttttt tctctaagag ctgaatggca
     5941 aattcttcga ttgtggattc ggtgatttta tttgtcattc gcttctccta atattcgttt
     6001 cacttctttg tcgaaatcgg aaatataatt ttcatcttgg gtgattcgaa aggtttcaaa
     6061 ttctttttcg gcttttaatt ttgcttctag catagaaact tttcctttgt ctgtaaggat
     6121 ttgatagttt gataattcta ggaaactatg tagaaatttt acccattctt ccattgttgt
     6181 ggctatttgt cgtttggctc tattttctgc taagtcaaga taggcagaaa taattcgatt
     6241 caattcttcc atatgcgctt tgtttaaata attctttgca atagaaacat cagatttaag
     6301 tatctttcca tcgggtgctt gtttccagtt tgtaagcccc atgtaaattt ttgtagcatc
     6361 agcagaatca taaataattt cagcggcagt ttttcctgta attgcccaat gtaatttatt
     6421 ttgaactgaa gcaaaaaaat ctttcgtagt tggagaattc ttatcgtaat ccgcagataa
     6481 cgcataaata tctgtaatct tctgataaaa ccgtctttca ctcgcacgaa tttctcgaat
     6541 gcgctccaga agttcatcga aataatcttt tccaaaatgc tttccttgct tgagtctttc
     6601 atcatccaac acaaatcctt tgatgataaa ttccttgagt gttttcgttg cccaaattct
     6661 gaattgagtt gcttggtagg aattaactct atagcccact gcgatgattg catctagatt
     6721 ataatatttt actgcctgtg tctgttgttt tccttctatt gccccatgct gagtggtatg
     6781 ttccaaaatg gaactaacca ctttttcctc taattctccg ctttcaaaga tgttctttag
     6841 atgcttagta atagcaggac gctttactcc aaaaagccgc gcaatacttt cctgagtgag
     6901 ccagactgtt tcatctttaa tgtatacgtc aatcttgata ttgccgtctg gtgaagtgta
     6961 aaagattatg ctagattggt tcattttctt tctcttctaa acaacttaca attttttcat
     7021 tgctttcatt attattcata atattttgaa aaagaacggt tgctagcgga tcgtcattcg
     7081 acggttccac cttaatgggg caatcgttta cttttttgct ttaatctaaa tctagcctca
     7141 aagaatttta gcaattctca atgcctgctt aacttcgata ttcatttagt ctggattgag
     7201 taaaattgga tttgcagtta aaatatctat acctgtattt ttaaaatctc catcatttgt
     7261 aagaaaaaat agtgagtttt cttgacaaaa ttttgcaatc agttggtcat taaaatctcc
     7321 attttcttta tctaggattt ctagaacttc ttctttagaa aaacttttat ctaagtattc
     7381 aaattgattt agaatttttt cttttgttat tgaatatatc tctttaactg tttccttccc
     7441 gtagttttga tctctaaact ctttgaatgg caaggatctc ttgtctaaat tattttccct
     7501 taaatgggtt tcatattcat atctaagtgc tctatttata aattccgata aagtagttct
     7561 atcagttacg attacaattt tttgtttaag taaatcttta aaaaaggaag agtattcttt
     7621 cacccatgat ggattgccca tcggccaaaa gatatataat aatatatttg catccatgaa
     7681 tactttttta tctttaattt tatcaattac atgtttacca attttttcaa tcatggattt
     7741 tctcctgtta tttcgcttaa cgttttttcc atccatgtcg ggtctttata atacaacttt
     7801 gcatttttta cgaccctctt aagtagagat ttatctacat ctgaaatgtt tgtgatagaa
     7861 accattttct tgatttcatc ctcagaaaag tcattgtaca attttcctat ggcagtattt
     7921 agaaatgcgg atgtaagcat atctacattt aaaaaactta tattcgcttt ggagttagat
     7981 tttaaagttt tatgtatcag ttcgtataca cgttctccgt cttctgcatc aacacaaatc
     8041 ggactaccca caatgctaaa tactgaaata tcaacggtat tcataaatcc tcctaaaaaa
     8101 tatcttccga gtcttcatct attaaactgt aactttgcgt atcatccgtt cgaaattgga
     8161 ctgtgactac agaaccagga aacgctccag agaaattttt gacaatttct cttttggaac
     8221 caaactgata gaacccagta tcacttataa tctgtatttt cccatcattt ttttcgacaa
     8281 attcttttaa aatagaaagt ccaattccac cacttgtacc tactttagtt gtatgaccgg
     8341 aagatactgc ccagcgaata gaatctgtag cacttaaatt tttattgaac ctattattga
     8401 tttttttctt aaatccaatt ccagtatcag tgattgtgaa ctcgatataa ttttttttcg
     8461 gaaagaactg tccgcaagta taaatatact tggtttcact atgcatttta gcatttgcaa
     8521 atagctcata aatggactcc gcaattttct ttttaagtgg aatcgacatt tcaggaagtt
     8581 cgggtctttc tattaattca tttaaaatat aatgattaaa aaatttcccg tcttttggtg
     8641 taagtttcaa ataaggaatc gttgtatggt gctcatctat cctttttgta tatccatagt
     8701 gtgccaaaaa accatttttt cgaataatcg tttctatatt ttgtggaaca tttttgattt
     8761 ggattttatt taaatttcca cttaatttat ctaatattcc tcccaatgcc gcactcatat
     8821 ttgccccaaa ccaatcttga aaagaaattt caattgtttg aaaaaatgat ttcttattcg
     8881 aatcatacaa ttcaatcaac tgttcaaaac tttcaaagga tgcgctaata ttttttaaaa
     8941 taaattcttt catggcttat ttgattttcg aatttcgaac gacgattttc gatttctgta
     9001 attcgacatt catacggtta ctctcacttc tccgctcatc agtttgggta ataacgtatc
     9061 gcggagtttg gttagggttt ggatttg
//
LOCUS       DAOQOS010000179         9035 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_4156_length_9035_cov_5.672494, whole genome shotgun sequence.
ACCESSION   DAOQOS010000179 DAOQOS010000000
VERSION     DAOQOS010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 9035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9035
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4156_length_9035_cov_5.672494"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..234)
                     /locus_tag="PLF66_23975"
     CDS             complement(<1..234)
                     /locus_tag="PLF66_23975"
                     /inference="COORDINATES: protein motif:HMM:NF014543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67168.1"
                     /translation="MLNKIYTIALLVFVITCANVEKRKGCITGNCDNGNGTFIDPEDG
                     KYIGEFRDGNFNGRGSFASADGDKYTGNFKDGLF"
     gene            complement(238..1518)
                     /locus_tag="PLF66_23980"
     CDS             complement(238..1518)
                     /locus_tag="PLF66_23980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000383076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HMZ67169.1"
                     /translation="MSHHPKGVFILFFTEMWERFSYYGMRAILFLYLVKYFQYDDEYA
                     GQVYGAYTGLVYLTPILGGYIADRFIGFKKAIFLGGCLMMFGHISLAFSSIEFFYLGL
                     ALLIFGNGFFKPNISTYLGNLYKDHPELKDAGFTIFYMGINLGAMFGPVLCGYLGEVY
                     GWHYGFGLAGIGMFFGLIVFNLGIKHLKMEEPVRIRLQNQSENKTLSEKEIRRILVIL
                     ILSIFTMFFWFAFEQAGSSMNLFADRYMDRTLFGFEIPASLFQSLNAFLILVFAPIFA
                     KLWSDLADRRKEPNTPVKFSIAFLLVGLGFLFLVFGSKEIDSNTNIKVSMVWLVFAYL
                     FHTLGELCISPVGLSMVSKLSPARFAGVLMGTWFLSNAIAHYIAGFFSGKMNQFASLS
                     DFFSIFVYTSFFSAVILFLLNKKINTMMDNDKQD"
     gene            complement(1515..2078)
                     /locus_tag="PLF66_23985"
     CDS             complement(1515..2078)
                     /locus_tag="PLF66_23985"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016544570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HMZ67170.1"
                     /translation="MKIYTKTGDKGKTSLASGKRVSKSDDLVSLYGTCDELNSQIGVA
                     ISFLLEDSTLKEPLFTTQNILFELGSELAGFRLNDSKESIIYNSDIEYLEIKIDELQS
                     KLTEMRSFILPGGAKSSGFLHVARTICRRLEREMVLAKEKNLEIFEMSLIYVNRLSDF
                     LFVAARFANQEESISDIKWTSRAKQNK"
     gene            complement(2141..2530)
                     /locus_tag="PLF66_23990"
     CDS             complement(2141..2530)
                     /locus_tag="PLF66_23990"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HMZ67171.1"
                     /translation="MAEAKQLFNKSFSVFDPKKQKRKKARVRLSTPGNYYLHSQKNRS
                     MSCHLIDLGTGGLTIQAPSPLYIGDRLTVEFILDGKTLRISGTVARATGKDYVIRYEE
                     LPPFETGIIQEYIHKVFFRDENKDKSK"
     gene            2618..4408
                     /gene="pabB"
                     /locus_tag="PLF66_23995"
     CDS             2618..4408
                     /gene="pabB"
                     /locus_tag="PLF66_23995"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008153 - para-aminobenzoic acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HMZ67172.1"
                     /translation="MFSKIVSDSPYSILLETTLTSKNESRNFFFQNPITVLEAYNLNQ
                     LEINLRKIEEYIEQGYVLAGYIAYEAGFLFFENLKNKFFSKNFPFPLLWLGVYPEENI
                     TKFSNSKEHIPSPASYKIENLKLNTSKLEYKNSIQKILDYIYSGYTYQVNFTIKTNFD
                     FIGDSSEFYEELKINQPVPYSAFLNDGRRKILSLSPELFFKREGNRITSKPMKGTLPR
                     GKSPLDDDRIKEQFQLDSKMKAENSIIVDLMRNDIGRITEIGSVNVSDLLIIEEYKTL
                     FQMTSTIQGELKSNTSYFDLFKAIFPGGSVTGAPKYKTMQIIQELEKGFRDVYTGAIG
                     FISKKESVFNLPIRTIVIENNFGTMGIGSGIISDSEPIKEFEECLLKQKFLINTLKFE
                     LIESILLVRNTYYLLRLHLNRLEKSAIYFGFSFNKKKIEEELNHFKINLDKKKRYKVK
                     LILSKNGEIKLDYTQIINEKAIGKIQIGSEKISSLNLFQMHKTTIRSFYNQMYKIAEE
                     LNITDFIFLNENEEVSEGSIYNLFIKKGKEYFTPPIDSGILNGVMREYLLMKNKLIEK
                     TLFKRDLLEADSIYLCNSVRGIIHVNLLTN"
     gene            complement(4505..4948)
                     /locus_tag="PLF66_24000"
     CDS             complement(4505..4948)
                     /locus_tag="PLF66_24000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67173.1"
                     /translation="MIKKILFSLTIIAISVSVSFCGKKEEPKEEVKEVKEEAKQADSS
                     NDLSAKIKAYEDFVTKFCALSEKMKGASVAEKATMSADFAKDSGNLKTLEADLEAAKA
                     SPDEKARIAAASKKASGCAAVAAGGSAPSSKTSELPVKTPSIPGM"
     gene            complement(5001..7175)
                     /locus_tag="PLF66_24005"
     CDS             complement(5001..7175)
                     /locus_tag="PLF66_24005"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HMZ67174.1"
                     /translation="MSWRKINRTLNIENLPNESAAYALVRYYEEHNEKSSVKNRLLYG
                     IITGIYPKEVGRAEINHLLTNPLKDSRVIAKLSYLKLYKEFVKQKQLTESEKIVYLQK
                     YTMENDPVVIKAFEEILKLHLDTKEYDQVLKRIDSLTEEEKKYIFTPEVKYIYAISLY
                     HSGKKEEGKKNLLSLFLDKNSNSSIKHKAMSGLKDFSNELTDYLTREELAAVILTLPI
                     KEQRSIAHRKIFNASQSYNNERLVKNITLFFLNTTTHNVLPFLKTNYNTISKEENFLA
                     NVAEDLINKRETRLAYDLQNTYLKNSTNPLVHKNYSRIHKKNKQEEKYFSSLLEYLKL
                     NPYDLRYHDSLIDFLANSDSKSIAYMPDEYWNRAIEAIPNLPVKGRLIYWYLRFLRFK
                     GETDKLKFILSSYYSYCPGSYYTSVIAEEFNSEIKTLPAVDNPTSSKENLFKYLSVNN
                     NSDYVKPLVGHNLNFAYYKDSIDLGQRLSNASSKIQSQKILLMAVEYLKVGEFDRGIY
                     IIDDYANRNNLNENEKFELYVGAGDLSKNAYLSLYYTRQLMKSFKIPDDPLLLPSSIT
                     TRLYPRPHLDIVKESAKEFSVDEDIVYAIMRQESFFRENAISPSNARGLMQVMPATGR
                     IIAKKLKIENYSLHDPEVSIKFGTKFIADLLNSYGALKWASIAYNGGPGNLRKWKRNH
                     YMNDFNHFLEELPSKESRDYCRIIISNYINYKVLRKLDGNGA"
     gene            7328..8683
                     /locus_tag="PLF66_24010"
     CDS             7328..8683
                     /locus_tag="PLF66_24010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67175.1"
                     /translation="MTEKKSKIIWEEFSFSEYTTESNVAVTTPKITKVQMSSLETENP
                     DRKSLKSKAEILDLKQTLKTSLSDSYDLTFIPEKFIQTNLLGEISFKRIEEIQFRSNY
                     LIQAKSEQDQKAISSFERIISYYDKEFIEDVEILEIVSTYLIQMNRLADLVALSNKFL
                     SKGILPLKFQIFFILTGIYENNLTNLVLSGNEKIIINILYKFKFLQIDKSEKNYLYDL
                     VVSGLNPELVGIVFQLFKDDYKGIRNISPVYKIISEKYKYLSREEKRAFVNSYEKTYN
                     YLKTYFYMKQSHSDLEIQTWLKAMKVMNGKDAAILDTIDQQLKDVPEKLKLNKKYLIL
                     KEHNASFLLNPFEILLLCNSTVALELKNEIADYYEKYPNSFIANRSIAVTYFHEEDYK
                     KFLMQIAQSGNFRYQMEVLYLKAIAYRELNLEKESSKIFQALYKRFPESEILAKEMGL
                     V"
BASE COUNT         3167 a         1505 c         1338 g         3025 t
ORIGIN      
        1 aaagagtcca tctttaaaat tgccagtgta tttatcccca tctgcagaag caaaactacc
       61 tcttccatta aaattaccat ctctaaattc accaatgtat ttaccatctt ctggatctat
      121 aaacgttcca tttccgttat cgcaatttcc ggtgatgcaa ccttttcttt tctcaacatt
      181 tgcacatgta attacaaata ctaaaagcgc tattgtatat attttattaa gcatatctca
      241 atcctgtttg tcgttatcca tcatggtatt tattttctta tttaacaaga ataaaattac
      301 tgcactaaaa aaagatgtat acacaaagat actaaaaaaa tcggagaggc ttgcaaattg
      361 gttcatcttc ccagaaaaaa atcctgcaat gtaatgagcg attgcattcg ataaaaacca
      421 agtccccatg agaactcctg caaatctagc tggtgataac ttagatacca tagataaacc
      481 aacaggggaa atacaaagct cacccaatgt atgaaataaa taggcaaata ctaaccaaac
      541 catactcact tttatattcg tatttgaatc aatttctttt gatccaaaaa ccaaaaataa
      601 aaatcctaag ccaactaaaa gaaatgcaat agaaaatttt acaggtgtat ttggttcttt
      661 tcttctatca gccaaatcag accagagttt agcaaaaata ggcgcaaaca caagtatcag
      721 aaatgcgtta agcgattgaa acaaagaggc tggaatttca aacccgaaca aagttcgatc
      781 catatatcga tccgcaaata aattcataga ggaccctgct tgttcaaaag caaaccaaaa
      841 aaacatagta aaaatggaca gaatcagaat tactagaata cgtcttattt ctttttcaga
      901 taacgtttta ttttcagatt gattttgtaa tcgaattcta actggctctt ccatttttaa
      961 atgctttatc ccaagattaa aaacgattaa tccaaagaac attccaatcc ctgccaatcc
     1021 aaacccataa tgccatccat aaacttctcc tagataacca caaagcacag gtccaaacat
     1081 agctcctaaa ttaattccca tatagaagat agtaaagcct gcatctttta attctggatg
     1141 atccttgtag agatttccaa gatacgtaga aatatttggt ttaaaaaacc cattcccgaa
     1201 aatcagtaaa gcaagaccta ggtaaaaaaa ttctatagaa ctaaaagcaa gagaaatatg
     1261 cccaaacatc atcaggcatc cccctaaaaa aattgccttt ttaaaaccaa taaatcgatc
     1321 cgcaatataa cctcccaata taggagtgag ataaactaaa cctgtatagg ctccatagac
     1381 ttgaccagca tattcatcat catattgaaa gtattttacc aaatacaaaa atagaatcgc
     1441 tctcattcca taataactga acctttccca catttctgtg aaaaataaaa taaaaacgcc
     1501 tttgggatga tgactcattt attttgcttt gctctagatg tccactttat atcagaaatc
     1561 gattcttctt gattggcaaa cctagctgcc acaaataaaa aatcagacaa gcggttaaca
     1621 tagattaaac tcatctcaaa aatttctaag tttttttctt ttgctagaac catttctctc
     1681 tctagtcttc tgcatatagt tcttgcgaca tgcaaaaacc cagaggattt agctccacct
     1741 ggaagaataa aacttctcat ttcagttagt tttgattgga gttcgtcaat ttttatttct
     1801 aagtattcta tgtcggaatt gtaaatgata gattctttgg agtcatttaa tcgaaagcca
     1861 gcaagttctg atcctagttc aaataaaata ttttgtgtag tgaataacgg ttcttttagt
     1921 gtagaatctt ctaataaaaa ggatatagcg acacctattt gggaattcaa ttcatcacat
     1981 gttccataga gtgatactaa atcatccgac ttcgaaactc gttttccgga tgctaaagat
     2041 gttttccctt tgtcccctgt tttggtataa atcttcatgg aaactacaat cctagaaaat
     2101 aaacagaggt cattcctttt ttattaggaa ttagttgtag ttatttcgac ttgtctttat
     2161 tttcatctct aaaaaaaact ttatgaatat actcttgaat gattcctgtt tcgaaagggg
     2221 gaagctcttc gtatcgaatt acataatcct ttccagttgc tcttgctact gttcctgaaa
     2281 ttctcaaagt cttaccatct aatataaatt cgacagtcaa tctatctcca atgtagagag
     2341 gactcggagc ctgtatagtt aaaccgcctg ttcctaaatc gattaaatgg caggacatag
     2401 aacgattttt ttgtgaatgt aagtagtaat tcccaggtgt ggagagtctt accctcgctt
     2461 tttttctttt ttgctttttg gggtcaaata ccgaaaaaga tttattaaat aactgttttg
     2521 cttctgccat agaatccgcc ttaaaaaaat ttcgcttatt atactttttt ttagattttg
     2581 tcaatatact ttttaaacaa gatggttttt tgatcctatg ttttcaaaaa ttgtgtccga
     2641 ttctccctat agcattcttt tagaaacaac tcttacatca aaaaatgaat ctagaaattt
     2701 ctttttccaa aacccaataa ctgttttaga agcttataac ctaaatcaat tagaaattaa
     2761 tttaagaaag atagaagaat acatagaaca gggatatgtt ttagccggtt acatagctta
     2821 cgaagctggt tttttatttt tcgaaaatct aaagaataaa ttcttttcga aaaactttcc
     2881 ttttccgctt ctatggcttg gcgtttatcc agaagaaaat ataacaaaat tttctaacag
     2941 taaagaacac attcctagcc cagcttccta caagattgaa aacttaaagc taaatacttc
     3001 gaagcttgaa tacaaaaaca gtattcaaaa aattttggat tatatttact ccggttatac
     3061 ttaccaagtt aattttacaa tcaaaactaa ttttgatttt ataggagact catctgaatt
     3121 ttacgaagaa ctgaaaatca atcagcctgt tccctactca gcttttttaa atgatggcag
     3181 aagaaaaatt ttatcactct ccccagaact tttctttaaa agggaaggaa atagaattac
     3241 ctcaaaacca atgaaaggaa ctcttcctag aggcaaatca ccattagacg atgatagaat
     3301 aaaggagcag tttcaattag attcgaaaat gaaagcagaa aatagtatca tcgtagactt
     3361 gatgcgtaat gatattggta gaatcaccga gataggatct gtaaatgttt cagacctttt
     3421 aatcattgaa gaatataaaa ccctctttca aatgacgtct acaattcaag gtgaactcaa
     3481 gagtaacact agttatttcg atctattcaa agcaattttt ccaggaggct ctgttacggg
     3541 tgcccctaag tataaaacca tgcaaatcat tcaagaatta gaaaaaggtt ttagagatgt
     3601 atacacagga gctattggat tcatatcaaa aaaagaatct gtctttaact tgcccatccg
     3661 aacgatagtc attgaaaaca attttggaac aatgggaatt ggaagcggaa ttatttctga
     3721 ttctgagcca ataaaagaat ttgaagaatg tctactcaaa caaaaatttt taatcaatac
     3781 tttaaaattt gaattaatag agagtatctt actcgtaaga aatacatatt acttacttag
     3841 attgcatttg aatcgattag aaaagtcagc aatttatttt ggattttcat ttaataagaa
     3901 aaagatcgaa gaagaattaa atcattttaa aataaatttg gataagaaaa aaagatataa
     3961 agtaaaacta atcctctcta aaaatggaga aataaaatta gattatactc agattataaa
     4021 cgaaaaagcg ataggcaaaa ttcaaattgg ttccgaaaaa atctcaagtc tgaatttatt
     4081 tcaaatgcat aaaacaacca ttcgaagctt ttataatcaa atgtataaaa tagctgaaga
     4141 actaaatata actgatttta tttttctaaa tgagaatgag gaagtatcag agggaagtat
     4201 ttacaatttg tttatcaaaa aaggaaaaga gtatttcact ccacctatcg attcaggtat
     4261 tttaaatgga gtaatgagag aatacttatt gatgaaaaat aaactcattg aaaaaacttt
     4321 attcaaaaga gatttactag aggcagattc tatctactta tgcaattcgg ttcgggggat
     4381 aatacacgta aaccttttaa ctaactaaat ctacgaagga aatattgata atgagtgtat
     4441 tcgacttaaa aacagatgat agtttgatgt aggctgtaga caaccaacat caaactaata
     4501 aggattacat tcctgggatg gatggagttt ttacaggtaa ctcgctcgtt ttactagatg
     4561 gagcacttcc accagctgct acagcagcac aaccagaagc ttttttggaa gctgctgcaa
     4621 ttctagcttt ttcatctgga gatgctttag ctgcttctaa atcagcttct aaagttttta
     4681 aattaccaga atcttttgcg aaatcagcag acatagtagc tttttctgca acagaagcac
     4741 ctttcatctt ttcagataac gcacaaaact ttgttacaaa gtcttcatac gctttaatct
     4801 ttgcagataa atcattagag ctatcagctt gcttagcttc ttctttcact tcttttactt
     4861 cttcttttgg ctcttctttt tttccacaaa aagaaacgga aacagaaatc gcgatgattg
     4921 ttaaactgaa taatattttt tttatcataa acctatccta aatttgttta gtttattatg
     4981 aaaaaaagta gaaaacaatg tcaagcgcca tttccatcta actttctaag aactttataa
     5041 tttatataat tggagataat aatcctacaa tagtctcttg attctttaga tggtagctct
     5101 tctaaaaaat gattaaaatc attcatataa tgatttcttt tccactttct taaatttcca
     5161 ggtccaccgt tataagcaat cgacgcccat ttcaaagctc catatgaatt gagcaaatct
     5221 gcaataaatt tagttccaaa tttaattgaa acctcaggat catgaagaga atagttttca
     5281 atttttaatt ttttggcaat gatccttcct gtagccggca taacttgcat taaccctctt
     5341 gcattagaag gagaaattgc attctctcta aaaaaggatt cctgtctcat aatcgcataa
     5401 acaatatcct catctacaga aaattctttt gcactttcct ttacaatgtc tagatgaggt
     5461 ctagggtaaa gtcttgttgt gatagaagaa ggaagtaaaa gaggatcatc tgggatttta
     5521 aaacttttca ttaattgtct tgtataataa agagaaagat aggcattttt acttaaatcc
     5581 cctgctccta cgtatagctc aaatttttca ttttcattca gattgtttcg atttgcataa
     5641 tcatctataa tgtaaattcc tcgatcaaat tcaccaacct ttaaatattc aactgccatc
     5701 aaaagaatct tttggctttg aattttacta gaagcattgc taagtctttg ccctagatca
     5761 atcgaatctt tgtaataggc aaagttcaaa ttatgaccaa caagtggctt tacataatct
     5821 gaattattat taacggatag atacttgaaa agattttctt tactggaagt tggattatct
     5881 acagcgggta acgttttaat ttcagaattg aactcttctg caattacgct tgtataataa
     5941 gaaccaggac aataggaata ataggaactc aaaataaatt ttaatttatc tgtctcacct
     6001 ttgaatcgta aaaatctgag ataccagtag atcaatcttc cctttacagg taaattggga
     6061 attgcttcaa tcgctcgatt ccaatattca tctggcatat aggcaataga tttagagtca
     6121 gaatttgcta ggaaatcaat gagagagtca tgatatctta aatcatatgg atttaatttt
     6181 aaatactcta atagacttga aaagtatttc tcttcttgtt tatttttttt atgaatacgt
     6241 gaatagtttt tatgcactaa aggatttgta gagtttttta aatatgtatt ttgaagatca
     6301 taagcaagtc ttgtttctct tttgttaatt aaatcctctg ctacgtttgc aagaaagttt
     6361 tcttcttttg atattgtatt ataattagtt tttaaaaaag gaaggacgtt atgtgttgtt
     6421 gtatttaaaa aaaatagagt gatattttta actaatcgtt cgttgttata agattgagat
     6481 gcgttgaata tttttcgatg agcaattgat ctttgttctt taattggaag ggtcaaaatt
     6541 acagctgcta attcctctct agttaagtaa tctgtcaact catttgaaaa gtctttcaga
     6601 ccactcattg ctttatgctt gatggaagaa tttgaatttt tatctaagaa cagagagagt
     6661 aagttctttt tgccttcttc tttttttcct gaatgataca agctgatcgc ataaatatac
     6721 ttaacctcgg gagtgaaaat gtattttttc tcttcttctg ttaaggaatc aattcgtttt
     6781 aatacttgat catattcttt tgtgtccaaa tgaagtttta aaatttcttc aaaagcttta
     6841 attacgacag gatcgttttc cattgtatat ttttgtagat atacaatttt ttctgattca
     6901 gttaattgct tctgcttcac aaattctttg taaagtttta ggtagctcaa tttagcaatg
     6961 actctagaat cttttaatgg atttgtaagt aagtggttga tttccgctct gccaacctct
     7021 ttaggataga ttccggtgat aattccataa aggagcctat tttttacaga gcttttttca
     7081 ttgtgctctt cataatatcg caccagtgca taggcagcac tttcattggg gagattttca
     7141 atatttaaag ttcgattgat cttacgccaa ctcatggatt tgatcagata atgaatttcg
     7201 tccgttgcaa aaacattttg agtgcaaaaa attaggatga ttgaaaaaat agatatttta
     7261 aagatggaca tgtatttaaa atgacaaaaa atacaatgcc tgaatgctga ctatttgaat
     7321 tcactctatg acagaaaaaa aatctaaaat catttgggaa gaattttcct tttcggagta
     7381 tacgacggag agcaatgtcg cagttactac tcccaaaatc actaaagtcc agatgtcttc
     7441 tctagaaaca gagaatccag acagaaaaag tttaaaatcg aaagcagaaa ttttagattt
     7501 aaagcagaca ttaaaaacat ccctttctga tagctatgac ctcaccttta ttcctgaaaa
     7561 attcatccag acgaatttac tcggagagat ttccttcaaa agaattgagg aaattcagtt
     7621 tcgatctaat tatttaatcc aagccaagtc agaacaagac caaaaagcaa tttcaagttt
     7681 tgaacgaatt atttcttatt atgataaaga atttattgaa gatgttgaaa tcctagaaat
     7741 tgtaagcacc tatctcattc agatgaatcg acttgctgat ttagttgctc tatccaataa
     7801 atttttatct aaaggaattc ttcctcttaa gtttcagatc ttttttattt tgacaggtat
     7861 ttatgaaaat aacctaacaa atttagtttt gtctgggaat gaaaaaatta ttatcaatat
     7921 tttgtataaa ttcaaattct tacaaataga caaatcagaa aaaaattact tatatgattt
     7981 agttgtttct ggtttaaatc cagaattggt tggaattgta tttcaactct tcaaagatga
     8041 ttataaagga attcgaaata tttctcctgt ctataaaatc atctcagaga aatataaata
     8101 tctatctaga gaagaaaaaa gagcctttgt aaattcctac gaaaaaacct ataattatct
     8161 aaagacttat ttttatatga aacaaagtca ttctgactta gaaattcaaa cttggctcaa
     8221 agctatgaag gttatgaatg gcaaagatgc agcaatatta gatacaatag atcaacaact
     8281 caaggatgtc cctgaaaaat taaagctaaa taaaaagtat ctaattctga aagaacacaa
     8341 tgcaagcttt ctactcaatc cttttgagat tctgctttta tgcaactcta cagtagcgct
     8401 agaattaaaa aatgaaattg cggattacta tgaaaaatat ccaaactcat ttattgcgaa
     8461 tcggtccatt gcagttactt attttcatga ggaagactat aaaaaatttc taatgcaaat
     8521 agcccaatct ggaaatttta gataccaaat ggaagtatta tatttaaaag ctatcgccta
     8581 tcgcgaactc aatttggaaa aagaatcctc taaaatcttc caagcactct acaaacgctt
     8641 tcctgaatca gaaatcctag caaaagaaat gggacttgtt taaccttttt taagccactg
     8701 attaacacag ataaacacgg attagctttt ttattttgga atagaaattt agcaaacgct
     8761 agcaatacaa atatgccaag taatttgtta cgaatagttt ttaacgcgag ttcaatggaa
     8821 tagaaaagag tattagaaat tatttttaac agctatatca gcagggtata tactggtaaa
     8881 cgtggaatat tcaaaagatt gaattcatct tttatccgtg tttatccgtg gtaaaatttc
     8941 ttgagtggtg tgtaagaact tagagtttac taaattctta cactattgta ttggtgggtt
     9001 aactgatctt agctgctact aattcagcta tatct
//
LOCUS       DAOQOS010000180         9025 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_4169_length_9025_cov_7.004125, whole genome shotgun sequence.
ACCESSION   DAOQOS010000180 DAOQOS010000000
VERSION     DAOQOS010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 9025)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9025)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9025
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4169_length_9025_cov_7.004125"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..713
                     /locus_tag="PLF66_24015"
     CDS             <1..713
                     /locus_tag="PLF66_24015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67176.1"
                     /translation="NTNTATTFGSFIGGGKWVGGVLAPNGKIYCIPADSTSVLVIDPA
                     TDTTYTFGSLSGTVAKWYGGVLAPNGKIYGIPNNETSVLVIDPNTDSISTFGSLVGGN
                     KWRGGVIAPNGKIYGIPANSATILEIDPTNDTTVTFGALGGTTLKWQGGVLSTNGKIY
                     GIPYNTDRVLILDPTTNTTAISSSLGGTFRWTTGALAPNGKIYGMPANNGQTLVIDPT
                     SNGTWPSDLYLSPYFNKL"
     gene            complement(817..1032)
                     /locus_tag="PLF66_24020"
     CDS             complement(817..1032)
                     /locus_tag="PLF66_24020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67177.1"
                     /translation="MREWAYKRAGCKVCDIRCNYEEWKNRKYAVRGHITIVYRLMKYI
                     KHQEKLPRRILYDWHSVEDNVYRGYLN"
     gene            complement(1659..2006)
                     /locus_tag="PLF66_24025"
     CDS             complement(1659..2006)
                     /locus_tag="PLF66_24025"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HMZ67178.1"
                     /translation="MNVIRDIQESPFGSMAGAEFHAHTLNTILNQEFITHSGDIWNLF
                     ILLFLGFIFGIATIYLSGLKRIFSYYLLCTLFNTIAILVFINFSILIPMVASNLLMLF
                     LIQILPFLPHKKN"
     gene            complement(2057..2467)
                     /locus_tag="PLF66_24030"
     CDS             complement(2057..2467)
                     /locus_tag="PLF66_24030"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HMZ67179.1"
                     /translation="MKIFLTTSFLSIFFYLCSIFILPIRHLEESSISLRFKWFRSYVG
                     KKVIHHAGNTIETFNRHPKSRRDISIIGIDTNTLDKLKGEWPIPLNYYGNIINAFQNT
                     PHTPVFDIFFIFPRRGFEQFTKSISKNPNGMELD"
     gene            2854..3114
                     /locus_tag="PLF66_24035"
     CDS             2854..3114
                     /locus_tag="PLF66_24035"
                     /inference="COORDINATES: protein motif:HMM:TIGR02607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HMZ67180.1"
                     /translation="MIKNPKPLHPGQVLFEIYMKEMGLNQSQLAEKCKCSPRKINEIV
                     NGKRAISPDFALVLEKVIGTTPEMWVRMQAEYDLFIARKKAA"
     gene            3296..3988
                     /locus_tag="PLF66_24040"
     CDS             3296..3988
                     /locus_tag="PLF66_24040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67181.1"
                     /translation="MPRKSFLFILQIFLIQLILLNCKEEKEKEEIDKINKVDKIKKTE
                     CSKKELLSKGNLEDFTIVDNCLSENDKKILIESIELFKKQKEDILKIISFKRSFIPII
                     KYLNIDLSYLNELASEYTPGENQTEEQMEKEYEETLNLVLKGELHSDSVGSLEYRGEK
                     RKLLVLDKIINFEVLIQVLKTNKFELLDDDFICIKQNIKNTENTLRIGFKLDKKKKIW
                     QLIDILIFYDNY"
     gene            4601..4882
                     /locus_tag="PLF66_24045"
     CDS             4601..4882
                     /locus_tag="PLF66_24045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67182.1"
                     /translation="MKQLTLIVSMAMLVSIQSLSAKCQYNSDWQKSDGKRIIVCVKGD
                     SFSDRDKARAICAKIKGSSCSVASSFSSSCSNNECYDENGKQSNSLSGY"
     gene            complement(5134..5760)
                     /locus_tag="PLF66_24050"
     CDS             complement(5134..5760)
                     /locus_tag="PLF66_24050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67183.1"
                     /translation="MAKPIIYTNHRSPSSLSVVVSFNILVGKDWMRLGVLLIMGFLLN
                     CSEISIREIKEGKHLDKAKSSTVDHFLQLRMKNSIKKIIAHNTEIILERPDFIYYGFV
                     KGGNWDNLYVEELFKVSHPELQKFFPGFRNLTGVEIKREIYSSDSFKLKHEHTMLQNM
                     DYIFSLTEKGLEVQVDWKYSVDGKPEEQKRFFILIDSKTGKRISEKQL"
     gene            6371..7462
                     /locus_tag="PLF66_24055"
     CDS             6371..7462
                     /locus_tag="PLF66_24055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67184.1"
                     /translation="MNIKQHKKKKIKRLIETLVFSILILVGSNRQLLSESAKQTKSIQ
                     ITSVSKAIEIFRDLVLVEPINCLVAIIAKKENIICIANKAGVDSNNKMFYSNPEIVYY
                     KLSKFADVWRVEKQAPVYKKEFFYWNSLPDFEIVTIGKKSYLFFHFLVSPMGNAVNLD
                     ELNFALFSLTDFRLTTLKYEGDPYTKNEKLFYLKGDFMNLNQLNTKPKLLKYLEKRAS
                     KSPWIYRTTKSDLEINSSTNYEKKWRIDNSSIKQVWEYKENILEQSIQITYYDKNIFP
                     PSYQGDTPRKIENSKYKIISLFRGDILGYDKTKEKYFPIWVDSCSHGCDKDISFISED
                     TLKIVYSESANKEVVSIDLKKMTYSISME"
     gene            complement(7768..8547)
                     /locus_tag="PLF66_24060"
     CDS             complement(7768..8547)
                     /locus_tag="PLF66_24060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002972809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
                     /protein_id="HMZ67185.1"
                     /translation="MKDIEIFLDQTSEILSKDPRIIALCVGGSWLTKEIDSYSDLDLV
                     IISKEDLSSSKENMVNIANRLGSLCSAFTGEHVGEPRLLICLYENPILHVDLKFVQLE
                     DYHTRVENPTIIWEKDRIITKAIENSIAKWPLPDLQWIEDRFWVWIHYAATKLGRGEY
                     FETIDFISFLRTNVLGPLLHMKYGSLPRGVRKLEFLLNEQDQTKLRATIPEYSFHSIK
                     LAILTIIDIYKELQTTLFDKGIHKLPKAESISILYLNNIKK"
BASE COUNT         3122 a         1425 c         1467 g         3011 t
ORIGIN      
        1 caaatacaaa tacagcaacg acctttggta gttttattgg aggaggcaaa tgggtcggtg
       61 gcgtgcttgc cccaaatggg aaaatctatt gcattcctgc ggattctacc tccgttctag
      121 tcattgatcc tgctacagat acaacgtata cctttggttc tctaagtggc acagtagcga
      181 aatggtatgg tggggtttta gcacccaatg gaaaaatcta tgggattcca aacaatgaaa
      241 caagtgttct tgtgattgat ccaaatacag attctatttc gacgtttgga agtttggtcg
      301 ggggaaataa atggcgtgga ggagtcattg cgccaaatgg aaaaatttat gggattccag
      361 ccaattcagc cactattctg gaaatcgatc cgacgaacga taccactgtt actttcggtg
      421 cacttggtgg cacaactcta aaatggcaag gaggagttct ttccacaaat ggaaaaattt
      481 atggaattcc ttacaataca gatagagtct taatcctcga ccctactaca aatacgacag
      541 ctatctcaag ttctctcgga ggaacatttc gatggacaac aggtgcactt gcccctaatg
      601 gaaaaatcta cgggatgcca gcgaataatg gacaaacctt agtgatagat cccacttcaa
      661 acgggacttg gccttccgat ctgtatttat ctccttattt taataaactt taatttctag
      721 gtctaatcac aatttagatg ttaaaataga gaattatcac agaggcaatt tatctgttgg
      781 atgtatttca tacaagcagg aatccttctt cgatttctaa tttaaataac ctcggtaaac
      841 gttatcctct accgaatgcc aatcatataa aatccttctg ggaagtttct cttgatgttt
      901 gatatatttc attaatcggt agactatcgt tatatgtcca cgaacagcat actttcgatt
      961 cttccattct tcataattac agcggatatc gcatactttg catccagctc ttttataagc
     1021 ccattctctc atacaagtta tagtcttaaa atacaaaatt ctgaattagc ccagatggaa
     1081 tttaggtaaa cttatttttt tacttttcaa tcacttgtgt aaaggaactt attatggaaa
     1141 taacaaattc atttaacaaa ttattaaatt tatctattct aaacattctt taaaatatcc
     1201 tctaggatag gaacgtgttt agtctaataa aatagtttgc gatttaattt tttaaaggga
     1261 tcagttatcc atgagaaaaa tcaactcttt agtattattt attacaatgc ttttttttgc
     1321 ttgcagtagt gaaagtccgg atgattttcc atgtaatcag aatataagct ttcccgagag
     1381 caaacaaaga gtttgcaatc ctaggcaact aaaacgctta gagaagtcag agtccgacag
     1441 aaaagtaaaa caaatttgtg ccttactctc agcagaatgt attcaaaatt gttctatcga
     1501 ttccaatcct gattcttgta taaggacaag ttgtaaaaca ttcttaattt gtacgtctgg
     1561 tatttatttg ccacctttca tgtttcgata aatctaaata ttttttattt cttgcggata
     1621 acgtctaatc tccatacaac ttttcccata atcatctact aatttttttt atgaggtaaa
     1681 aaaggtaaaa tttgtattag aaaaagcatt aagaggttgc ttgcaaccat cgggataaga
     1741 atagaaaaat ttataaatac aagaattgct attgtgttga atagagtgca tagaagataa
     1801 taagaaaaaa ttctttttaa gccagataga tagattgtcg cgatcccaaa aataaaacct
     1861 aaaaataata agataaacaa attccaaata tctccgctat gggtaatgaa ttcttgattt
     1921 aaaattgtat ttaaagtatg agcatgaaat tctgcaccag ccatagagcc gaagggagat
     1981 tcttggatgt ctcggatgac gttcattcca gttgggtaat aggaagaaat taaataaatt
     2041 gtcttaggta tttcatttaa tccagttcca tcccattcgg atttttagaa atagactttg
     2101 tgaattgttc aaaacctctt cgtggaaaaa taaaaaaaat atcaaacacg ggagtatgtg
     2161 gggtgttctg aaaggcattg ataatatttc cataataatt caagggtatc ggccattccc
     2221 cttttagttt atctaaagta tttgtatcaa ttccaataat ggaaatatct cttctcgatt
     2281 tagggtgacg attaaatgtt tcaatggtat ttcccgcatg atgtataact ttttttccca
     2341 cataggagcg aaaccattta aaacgtagag aaatcgaaga ttcttctaag tgccgaattg
     2401 gtagaataaa aatagaacat aaatagaaga atatagaaag aaaacttgtc gtcaaaaata
     2461 ttttcattgc aaggatagaa aagggaaaaa gagaattagt caatagattt tttgcttgca
     2521 acatttccca ttataccaca tagtgtaata gttaatgatt aggaattttg ctaataaaga
     2581 tacggaagaa ttatgggaaa ctaatgcttg taaaaaattt cccagagaac tttggatgag
     2641 agcaaaagca cttttaacaa ttatgcatag tacttctact ttggatgatc taaaaattaa
     2701 aggtcagcca tctaacatta ggcttcataa actaaagggc aataggaaat tagaatggag
     2761 tattacaatt catcttccct ggtgtattac gttccagttt aaagatggcg aatttttaga
     2821 tgttaaaata gagaattatc acagaggcta attatgatca aaaatccaaa accattacac
     2881 cccggacaag ttctttttga aatttacatg aaagagatgg gcttaaatca atctcaatta
     2941 gcagagaaat gtaaatgttc tccgcgtaag ataaatgaaa tagtcaatgg gaaacgagct
     3001 attagccccg actttgcgct cgtattagaa aaagtaattg gcactactcc agagatgtgg
     3061 gtaaggatgc aggcggaata tgatctcttc attgcaagaa aaaaagctgc ttaattttat
     3121 tccttgcggc tatcgcttaa tttgtatcga acttctccca tcatagtctc ccattctatt
     3181 ttacaagtga atgccttaaa tcaaaaatag attgcaagtc tttcataaaa agatgttttg
     3241 aaatagacgg tgtaaagttt gcattttgag ttcaaagaaa gaaggagata atgatatgcc
     3301 tagaaaatct tttttattca ttttgcaaat ttttttgatt caattgattt tactcaattg
     3361 taaggaagaa aaagagaagg aggaaataga taaaatcaat aaagtagata aaataaaaaa
     3421 aacagaatgt tcaaagaaag aattactttc taaaggtaat ttggaagatt ttacaatcgt
     3481 tgataactgc ttatcagaaa atgataagaa aatattaata gaatctattg aactatttaa
     3541 gaaacaaaaa gaggatattc ttaagataat ttcttttaaa agatctttta tacctattat
     3601 aaaatatttg aatattgatt tatcatactt aaatgagcta gcatctgagt atactcctgg
     3661 cgaaaatcag acagaagagc aaatggagaa agagtatgaa gaaacattaa acttagttct
     3721 caaaggagaa ttgcactctg attctgttgg cagtttagaa tatagaggag aaaagcgaaa
     3781 gctattagtt ttagataaga ttataaattt tgaagtattg attcaagttt taaaaacgaa
     3841 caaatttgaa ctacttgatg atgactttat atgcataaaa caaaatatta aaaatactga
     3901 gaatactctt cgaataggtt ttaaattaga caagaaaaaa aagatttggc aattaataga
     3961 tattctgatt ttttatgata attattaata tttacttgtg gtattctagc ggctaacgcc
     4021 taatttatat ctaacttttt tcaatatcgt ctattattct ttcttacaag ataaagcatt
     4081 aaatctaaaa aggattgcga gtgttttaga aagagatgtt ttgaattaga tgggggaaat
     4141 tttgttttat gcagaatttg taggttgcaa aatttattct atcatgaaga tagaatttta
     4201 tctttctgac aatctaaaag gttaagttat tttcaaaaag acctactata aaaaaatctt
     4261 gccgtggata aagaacctat gtccattttg aggtcaagga accaagcact aactgaaatc
     4321 ctaatagctt tggaaaatga tctgagagga attttctttt aaatagagtt gttgaaaaat
     4381 tcagtagacg taattaattt cacttcgagt ttaatcgaga agtgctaata aaataatgct
     4441 ttttaatatt gtagttttcg acgatcgata gaacttacat cggagctatc taatttttca
     4501 aaaactctaa taaagatttc tatttaaaag aaaaaatatt ttgactttat aataataaat
     4561 tctaaacttt atagaatcta aacaataaag aggaaaaagt atgaagcaat taacattaat
     4621 cgtaagcatg gcgatgcttg ttagcattca atcattatct gcgaagtgcc agtataattc
     4681 agactggcaa aaatctgacg gaaagcgaat catagtttgc gtgaaagggg atagctttag
     4741 cgatcgagac aaagcaagag ctatatgtgc taaaataaaa ggttcttctt gtagcgtagc
     4801 tagtagcttc tcaagttctt gcagtaacaa tgaatgctat gatgaaaatg gaaaacaaag
     4861 taactcactt tcaggttact aatatttttc actgatattt aaacaatgaa tacaaatgtt
     4921 atttcagatt attattcgta ttcattgtat ttttattttt tcctttctag cggctaacgc
     4981 ctaatttgca tccaacgctt tccataatcg tctcgtattc tattttacta gcgaaagtct
     5041 ttaatcaaaa atggtttgca agtcttttat gaaaagacgt tacgatctaa acaaggcgaa
     5101 attttattag taaatcatct tacatgagcc aagttataat tgtttctctg aaatgcgttt
     5161 tcccgttttg gaatctatta agataaaaaa tcttttttgc tcttctggct tcccgtctac
     5221 gctatacttc caatctactt ggacttccaa acctttctcg gtcaacgaga aaatgtaatc
     5281 catattctgg agcattgtat gttcgtgctt caatttaaaa gagtcggaag aatagatctc
     5341 tcttttgatt tctactcctg ttagatttct aaaaccagga aagaactttt gtaattcagg
     5401 atgggaaacc ttgaaaagtt cttctacata tagattgtcc caattacctc cttttacaaa
     5461 tccataatat ataaaatcgg gtctctctaa aatgatttct gtattatgag cgattatttt
     5521 cttaatagaa tttttcattc taagttgtaa gaaatgatca acagtagagg acttggcttt
     5581 atctaaatgc tttccttctt tgatttctcg aatagaaatt tcagaacaat tcaaaagaaa
     5641 acccataatc aataataccc ctaatctcat ccaatcctta cctacaagta tattaaaact
     5701 gactaccacc gataaagacg atgggctccg atggttagta tatataattg gttttgccat
     5761 tttacatttt ttgtcgattt aatcacgttt aagcatctaa agattcaaat attcgtcctt
     5821 caaacacatt taaccaaagc aattaaaccg atatatgaat ccatcgtttt tatcgttctg
     5881 ttatcggtgt catcggtggt aatctgttaa aattgtgggt aaggatatgt aatctcatcc
     5941 aatccttaaa tttcagtatc agcatattgt ccattttcaa gtgcgtttat caaatagcaa
     6001 atatttattt tttatttcta gcggctaacg cctaaattcc atcgaacttc ttttataatt
     6061 ctctttcatt ctatcttaca gttgaatgcc ttaaagcaaa atggtttgcg aagcttttag
     6121 aataagatgt tttgaactaa acggggtaaa aattgttttt ggaattcaat tccaatgcct
     6181 cataattaaa actacgatcg aatatattta tactccaatc gaagtaattt ctcctaaaat
     6241 tgattttgaa gtgggaatcc ttccgaaata taaattgatt cgaattgcgt ttgagaagga
     6301 atattgtttg ttgaacaatt ataatgtaag atgtattatt caaaattaac tgtaatgaaa
     6361 ctgggaaagt atgaatatta aacaacacaa aaagaaaaaa atcaaaagac tcattgagac
     6421 gttagttttc agtatattga tattagttgg ttccaacaga caattactaa gtgagtcagc
     6481 taaacaaaca aaatcaatcc aaataacttc agtgagcaag gctattgaaa ttttcagaga
     6541 cctcgttctt gttgagccaa taaattgtct ggtagcaatc attgccaaaa aggaaaatat
     6601 aatttgtata gcaaacaaag caggagttga tagtaataac aaaatgtttt actccaatcc
     6661 agaaattgta tattacaaat tatcaaagtt tgcagatgtt tggagagtag aaaaacaagc
     6721 tcctgtttat aaaaaagaat ttttttattg gaactccctt ccagattttg aaattgtaac
     6781 tattggtaaa aaatcctact tattttttca ttttcttgta agtccgatgg gtaatgcagt
     6841 taatctcgat gaactaaatt ttgcactatt ctctttgacg gattttcgat tgactacatt
     6901 aaaatacgaa ggggatcctt atactaaaaa tgaaaagttg ttttatttga aaggtgattt
     6961 tatgaacctt aaccaactga ataccaaacc caaactttta aagtatcttg aaaaaagagc
     7021 cagtaaatcg ccttggatat atcgtaccac taaatctgac cttgaaataa atagttcaac
     7081 aaattatgaa aaaaaatggc ggattgacaa ctctagcata aaacaagtat gggagtataa
     7141 agaaaatatt ttagaacagt caattcagat aacctattat gacaaaaata tctttccgcc
     7201 atcatatcaa ggtgataccc ctcgcaaaat tgaaaacagc aaatacaaaa ttatttctct
     7261 ttttagaggc gatattttag gatatgataa aacaaaagag aaatattttc ctatctgggt
     7321 cgacagctgt tctcatgggt gcgacaaaga tatttcattc attagtgagg acactttaaa
     7381 aattgtttat tctgaatcag caaacaaaga ggttgttagc attgatttga agaaaatgac
     7441 ttatagtata agcatggaat gaagatagag atgttatact ggcttaaaag agaggcttta
     7501 tgaatagaat taaatctagt attgttaaat atcattttgt tatataatcc tattttcttt
     7561 tgtctcttgt gctactggat tacttgttaa gaacattgtc tttctaggaa tttgcatcca
     7621 acttctccca ttattctctc ccgtttctat cttacaagtt aaaggcttaa agcaaaaaag
     7681 gattgcgagt ctttcataaa aagatgattt gaactaaaag gggtaaaatt tttgctttaa
     7741 ggtaactcaa tgatccgcaa caagtcctta ttttttgata ttgttcaaat agagaatact
     7801 tatactttcg gcttttggta atttatgaat ccctttatca aatagggttg tttgtaattc
     7861 tttatatata tcgattatag taaggattgc taattttata gaatggaaag aatattccgg
     7921 gatagtggct cttaattttg tttgatcctg ttcgttcaat aaaaactcta atttgcgaac
     7981 accgcgagga agactgccat atttcatatg caataatggc cccaatacat ttgtgcgcaa
     8041 aaaagatata aaatcgattg tttcaaaata ctctcctcta ccgagctttg ttgcggcata
     8101 atgaatccaa acccaaaatc tatcttcaat ccattgaaga tcaggcaatg gccattttgc
     8161 gatagagttt tcaatggctt tcgttatgat cctatccttt tcccaaataa tggtcgggtt
     8221 ttcaactcta gtatgataat cctccaattg gacaaacttt agatcaacat gtaagattgg
     8281 attttcatat agacaaatca aaagacgtgg ttctcctaca tgctctccag taaatgccga
     8341 acataaggaa cctaatcgat ttgcaatatt taccatattt tctttggaac tggaaagatc
     8401 ttccttagaa atgataacta aatctaaatc agaataggaa tcgatttcct ttgttaacca
     8461 ggatccccca acacaaagtg caatgattcg aggatccttg cttaaaatct cagatgtttg
     8521 atctaaaaaa atttctatgt ccttcataat ggcttatcct ttggtttgca tttagtgaaa
     8581 cattttggtt acctagtatt tcacctgaat cgattctttg ctacatggaa aaatatcctt
     8641 tgcacggggt aaggggcaca gatttttttg ttcttatcga taaaaaattt ataaacaaaa
     8701 tctccctgta aaggttggga ccaaatgata tttttataga ttttcagttt actgagtcca
     8761 cattttccat ctccacaaat cgggtcaaaa tgataaaact ccccattttc caaaaacaca
     8821 taattcaatt taggttccga gttgaaattt tggaatttta tttccttttt caaatacaag
     8881 tccgtaaaca attcttcttt atactcaaca atatccttgg accttacata gcccttcgcc
     8941 gaattacatt catttgtttc atgtttcact tttctttcta gcggctatcg cctaatttgc
     9001 atcgaacttc tcccataatt gtctc
//
LOCUS       DAOQOS010000181         8945 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_4218_length_8945_cov_5.115973, whole genome shotgun sequence.
ACCESSION   DAOQOS010000181 DAOQOS010000000
VERSION     DAOQOS010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 8945)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8945)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8945
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4218_length_8945_cov_5.115973"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..686)
                     /locus_tag="PLF66_24065"
     CDS             complement(<1..686)
                     /locus_tag="PLF66_24065"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HMZ67186.1"
                     /translation="MKSILIFLSLFFTFSLLEANDSLTFIDAAAKGDLPTIKRNIENG
                     IDINTSDEDGNTALHLAVIKGNLELTQYLIQKGAKINQKDGNGDTPLANACVLGNVEI
                     AKYLIEHGAEIDSKNQYGWTPLRLASNEGYMPLVNFLLEKGADLSPLYIADKLFLDAA
                     KNGDLDGLKKYLTRGASVNAVDSEGNTALLYVTKPQDKPFINTLEMVKLLIESGADLE
                     DRNTIGETAL"
     gene            822..1925
                     /locus_tag="PLF66_24070"
     CDS             822..1925
                     /locus_tag="PLF66_24070"
                     /inference="COORDINATES: protein motif:HMM:TIGR02481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin family protein"
                     /protein_id="HMZ67187.1"
                     /translation="MLTETETLNPQHIANVKDFWKFQNFQLGIPFIDTQHIWLVSLVL
                     QLNAIIKTAHGDQLNDLFQKAVADAFEYADTHFQAEEALFSTFAFPEKDSHIKQHGAF
                     IHSLKKLVASSPRIDRFIAEKLYRYLRHWLSEHILKEDRKYAQYLLQKKSNIENFFEE
                     HAMDGIFILSLEQMELYNQVTSVKETTDTQDKNVILLGKIRDIWTRFKLKLDIPLIDI
                     QHLWLIKITFELEMRLHDKAENRQEDIKDILQEAMLYVSEHFHTEEYLMEKLNYQHLL
                     GHKYKHAGLVYSVLERKAEIEAGNERAANILAIDLKEWLFSHIALEDKKFSQTIKENR
                     NEGIRLSKMLIEEKQVKIRKGQLELYKNIVLGV"
     gene            2288..4297
                     /locus_tag="PLF66_24075"
     CDS             2288..4297
                     /locus_tag="PLF66_24075"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IGHMBP2 family helicase"
                     /protein_id="HMZ67188.1"
                     /translation="MNSTDELQELYRLLEIEQEYERNQYETLLSETSPAERQKNGVTW
                     YPVQIELEEVTSSERLILSIRIHPDKAKDHKFHSGQTVSIFTNKEGLSKEDKKKFRTY
                     GTIRKIKDEVMYVMLSESEMPDWFEEGKLGVDLFYNETSYEEMKFALTKVMAASKNRL
                     AELREIILGYKETSIEKEKFPIVKELNTSQNEAYAMALNAKDFAVIQGPPGTGKTTTL
                     VQIIKEAVEREKRVLVCAASNNAVDLLVEKLAYEGVNVLRIGNPARVLESSQDYTFDA
                     KIYSHPDFEKLQDYKKEVLQIKKKAYKFKRNFGKEQREERIALKSEAKELMGIIRDLE
                     INIEKDIVDKAEAICSTFVGITNSPASRIHFQTVFIDEATQALEPAVWISILRANRVI
                     FCGDHKQLPPVVLSPDAARGKLDVSLFEKILYRLERTVRQNNTEVQKEDKTSKGNLIS
                     SFPFVLLDTQYRMHEEIMNFSNEYFYHGKLIAHESVKNRTLDSVFGYSEKIPALRFID
                     TAGTDSEEILNEENLSLENPKEAELLFKQIQIDLEKWNWNELNRKKISIGILSPYNEQ
                     IKTLKDRFEETNLSKKYDIEISTIDSFQGREKDIIYISLVRSNEVGEIGFLSDTRRMN
                     VAMTRARMRLVVIGDSSTIGNHEFYKKFLEHVEKYNSYESAWDYY"
     gene            complement(4291..5403)
                     /locus_tag="PLF66_24080"
     CDS             complement(4291..5403)
                     /locus_tag="PLF66_24080"
                     /inference="COORDINATES: protein motif:HMM:NF015724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HMZ67189.1"
                     /translation="MLLFLHLTPIILLVIHLYGCASLGVDPHKIIVRDLSEPEEYKKK
                     TIAILPFDGSVKGFDSISFAEKLTHEFVNSGQYQIIERSKINRVLKEQSLSSQGITEA
                     DSIKIGKLLAAEGVVVGSIVKENSNTTILARIVDTETGKIWSSSIVILQAEKQTPAQS
                     NYTTGDNAFKPIPESEKPKAEIKDLQLIRSGNFGRFLGLLKNTGNIPLTGNKLYINLK
                     DKKNTFLDTVQCFTDKPVYPQEEVSFSCILSNFPNEYASHDAFFEAADKLYGNQTSFK
                     IMSEIFREDKSGLEGYSLSGILKNDTSSYITYPKVILLLFDQNKKFVGSAIGFGNQKK
                     LASGETTSFKVSAYNYTLSGKPTSYKVQTHALVVNQ"
     gene            5801..6574
                     /locus_tag="PLF66_24085"
     CDS             5801..6574
                     /locus_tag="PLF66_24085"
                     /inference="COORDINATES: protein motif:HMM:NF025053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HMZ67190.1"
                     /translation="MIIKASYITNYGNFRIKNEDSLLVQEEIIYEKNMEEPKNKILES
                     KKVILCVADGMGGHLGGELASRSVLSFLRDNFMKLYNARTIKKILLKSKNILNTIASE
                     TRSYGLGTTVSGMLLKDKKGIIFNCGDSRVYRVQKMQLERLTIDHSVVQAMYNSGLID
                     EEEMRTHPNKNLLTSAIIGDYSQNHPVIAMKEIPIKEGDQFFICTDGLWESMSAEELT
                     ELFFSCEDQDERALKLNQLSLDKGGRDNITMILFEILEL"
     gene            6851..7330
                     /locus_tag="PLF66_24090"
     CDS             6851..7330
                     /locus_tag="PLF66_24090"
                     /inference="COORDINATES: protein motif:HMM:NF025160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HXXEE domain-containing protein"
                     /protein_id="HMZ67191.1"
                     /translation="MIELLFLLGFSLHNIEEAIWLPSWSKNAGKFHPPVKQNEFMFAI
                     VIVTMLGYLGTFQFFIFQGVSNISKSIYLGFILMMVLNSIFPHLVATIYFKRYAPGTI
                     TALFLNLPIGLYLILTHLKTRQDLFYMVISAIIMIVLFMSGIEQIFKLGDKLFEERE"
     gene            7647..8321
                     /locus_tag="PLF66_24095"
     CDS             7647..8321
                     /locus_tag="PLF66_24095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67192.1"
                     /translation="MLRKFVFILLIGFSFLWAEDFEYPTLINPDGTIQRREFSKEQKT
                     TFDSIQKDVKYFHTELSKILPYLIREGKVKEKSYTREIRNFTETPPGRTNSHFVSENF
                     VIKLTNDGKVENISFLKRRSRLNPKMYSETVIRTISNDISNEEMTALKVQIDTYTNYT
                     DPSAPIRKLVSMSEIPSPLDRIRLLRTYRAKLEEAVREIDKLIDHKVLNDHIKLSNTL
                     NDIELE"
     gene            8325..>8945
                     /locus_tag="PLF66_24100"
     CDS             8325..>8945
                     /locus_tag="PLF66_24100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67193.1"
                     /translation="MNLEWILQAYIDTGIFISVFFILKEILHLKVNRQLGFHFWYAFL
                     AFIFQVKISILVKESYFVNPYLFLFSVSGLFIIGPFQRAQILKLFGKIQFRDLYQHYA
                     ILLITIPFEIFIIYYYPDQIPACDRVTYVGRVFSALGLIYYFVYNYQNERLFKNFLNQ
                     YKLEHTNLVLSYNRMVSVFVLFGAVSIFFSKKILFQIDGIFICTGAF"
BASE COUNT         3081 a         1342 c         1560 g         2962 t
ORIGIN      
        1 tgaagagctg tttcgcctat tgtatttcta tcctctaaat ctgctccact ttcaattaat
       61 agttttacca tctccaaagt atttataaaa ggtttatctt ggggttttgt tacataaagg
      121 agagcagtgt ttccttctga atcaactgca tttacgcttg ctcctcttgt tagatacttt
      181 ttcagaccat ccaaatctcc attttttgca gcatctaaaa ataacttatc tgcgatataa
      241 agaggactca gatcagctcc tttctcgagt aagaaattta ctaagggcat ataaccttca
      301 ttagacgcta aacgaagagg tgtccagcca tactggtttt tggagtcaat ttcggctcca
      361 tgctcgatta aatattttgc aatttcaaca tttccaagaa cacaggcatt tgctagagga
      421 gtgtctccat ttccgtcttt ttgatttatt ttagcccctt tttgaattag gtattgtgtc
      481 agttctagat ttcctttgat aactgctaga tgaagagcag tattcccatc ctcatcacta
      541 gtattgatgt cgattccatt ttcgatattt cttttaattg taggcaaatc tcctttagcc
      601 gcagcatcga taaacgtcaa agagtcatta gcctctaaga gagaaaaagt aaaaaaaagt
      661 gataaaaaaa tcaaaatcga tttcataaga ccctcgaaca aagtttgatt ctttctgttt
      721 tgctagcaag aaaaaaaagt aatgatattt atcatgttcc attcattcta tttcagaatc
      781 tttataattg atatatatta agtctcaaaa agaatagaag catgctcact gaaacagaaa
      841 cgctgaaccc ccaacacatt gcaaatgtga aggatttttg gaaatttcag aattttcaac
      901 tcgggatacc ttttatcgat acacaacata tttggcttgt atctctagtt ctacagttaa
      961 atgcaattat caaaactgca catggagatc aactcaatga tttatttcaa aaggctgtag
     1021 cagacgcttt cgaatatgcg gatacacatt tccaagcaga agaagctcta tttagcacat
     1081 ttgcatttcc tgaaaaagat tctcatatca aacagcatgg agcatttatt cattctttaa
     1141 agaagttagt tgcaagctct ccccgaatcg atcgttttat tgcagaaaaa ttatatcgat
     1201 atttaagaca ttggctttca gagcatattt taaaagaaga tagaaagtat gcacaatacc
     1261 tattgcagaa gaaatctaat atcgaaaatt tcttcgaaga acatgcaatg gatggaattt
     1321 ttattttatc tttagagcaa atggaattat acaatcaagt cacatctgta aaagaaacaa
     1381 cagatacaca agataaaaat gttatactgc ttggaaaaat tagagatatc tggactcgat
     1441 tcaaattaaa actagacatt cctttgattg atattcaaca cctgtggtta atcaagatta
     1501 cctttgaatt agagatgcga ttacacgaca aagcagaaaa tagacaagaa gatataaaag
     1561 atatattaca ggaagcaatg ctttatgtat cagagcattt tcatacagaa gaatatttga
     1621 tggagaaatt aaattatcaa catctactcg gtcataaata caaacatgct gggcttgttt
     1681 attctgtctt ggaaagaaaa gcagaaattg aagctggaaa tgagagagcc gctaatattt
     1741 tagctataga tctaaaggag tggctctttt cgcatatcgc attagaagat aagaaatttt
     1801 ctcaaactat caaagaaaat cgaaatgaag gaattcgtct ttctaaaatg ttgatcgaag
     1861 aaaaacaagt caagatcaga aaaggacaat tagaacttta taaaaatatt gttttaggcg
     1921 tttaaagcta agttactcac cttgcttctg tgtgcaattg catctagatt aggaaggatt
     1981 caaagattac caccgattaa accgataaca gaacgataat gacacgatgg gtaattttga
     2041 attattgtat cttttatcag tgataaaagt ccctacacat tcgaaatggc tcagtgcaag
     2101 tcagtggtaa tcttttaatt tttctgttac atcgaacagg tgctaaattt agtttacatt
     2161 ttcttagaag tttgcaaacc tagttatcga gttcgcatga aagtgtttga gaaaaaaata
     2221 cagcggcctc aatgcatgtg aaaatttcta tttcatccat ctatgaaaag gttaaattag
     2281 tataattttg aattcaacag atgaactaca agaactatat cggcttctag aaatagagca
     2341 agagtatgag agaaatcaat atgaaactct actttcagaa acaagtcccg cagagagaca
     2401 aaaaaatgga gttacttggt atcccgttca aattgaacta gaagaagtta cctccagcga
     2461 gcggttaatc ttaagtatcc gcattcatcc tgacaaagca aaagatcata aatttcattc
     2521 tggtcaaacc gtttccattt ttacaaataa agaagggctt tcgaaagaag acaaaaaaaa
     2581 gtttagaact tatggaacca ttcggaaaat caaagacgaa gtcatgtatg taatgctttc
     2641 tgagtcagaa atgccagatt ggttcgaaga aggtaagcta ggagtcgacc ttttttataa
     2701 tgaaactagt tatgaagaaa tgaaattcgc tttaacaaaa gtaatggcag cttctaaaaa
     2761 tagacttgct gagcttcgcg aaattatttt gggttataaa gaaacatcta tagaaaaaga
     2821 aaaatttcct atagtaaaag aattaaatac ttctcagaat gaagcctatg ctatggcgct
     2881 taacgcgaaa gactttgcag tcattcaagg acctcccgga actggaaaaa caactacact
     2941 tgttcaaatc attaaagaag cagttgagcg agaaaaaaga gttctagttt gtgcggctag
     3001 taataatgca gtggacttgc ttgttgaaaa attggcttat gaaggcgtca atgttttacg
     3061 gattggaaat cctgctcgag ttttagaaag ttcacaagat tatacttttg atgccaaaat
     3121 atattcgcat cctgattttg aaaaattaca agattataaa aaagaagttt tgcagattaa
     3181 gaaaaaggca tataagttta agagaaattt tggaaaagaa caaagagaag aaagaattgc
     3241 cttaaaatcc gaagcaaaag agctaatggg tattattcgt gatttggaaa ttaatatcga
     3301 aaaagatatc gttgataaag cggaggcaat ttgttctaca tttgttggaa ttacaaattc
     3361 tcctgctagt agaattcatt ttcaaactgt gtttatagat gaggcaacac aggctctaga
     3421 gcctgcagtt tggatttcga ttcttcgagc aaatcgagtc atattttgtg gagatcataa
     3481 acaactaccg cctgtcgttc tctcacctga tgcagctcgc ggtaaactag atgtatcctt
     3541 atttgagaaa attttatacc gactagaaag aactgttagg caaaacaata ctgaagttca
     3601 gaaagaggac aagacttcaa aaggcaattt gatttctagt tttccttttg ttcttttaga
     3661 tacccaatat cgaatgcatg aagagattat gaatttttca aatgaatatt tttaccatgg
     3721 aaaactgatt gcacatgaat ccgtaaaaaa tcggacctta gattctgtat ttgggtattc
     3781 agagaaaatt ccagctcttc gttttattga tacagcaggg actgattcag aagagatttt
     3841 aaatgaggaa aatttaagtt tagaaaatcc aaaagaagcg gaacttttgt ttaagcagat
     3901 tcaaatcgat ttagaaaaat ggaattggaa tgaattgaat agaaaaaaga tttctattgg
     3961 aattctttct ccttataatg aacagattaa aactctaaaa gacagatttg aagaaacaaa
     4021 tctctcaaag aagtatgata ttgagatttc tactattgat tcttttcaag gtagagaaaa
     4081 agatataatt tacattagcc tagttagatc aaatgaagta ggagagattg gatttttatc
     4141 tgatactagg cgaatgaatg ttgcgatgac tcgagcacgc atgcgtcttg tagtgattgg
     4201 ggatagttct accattggaa atcatgaatt ttataaaaag tttttagagc atgtggaaaa
     4261 atataactct tatgaaagtg cttgggatta ttattgattt accacgagag catgggtttg
     4321 aactttatag cttgtcggct ttccggataa cgtgtaatta taagcactta ctttaaagga
     4381 agttgtctca ccagatgcta atttcttctg atttccgaat ccgatagccg agcctacaaa
     4441 ttttttattt tgatcaaata aaagtaaaat tacttttgga taagtgatat aagaggatgt
     4501 atcatttttt aaaataccag ataagctata tccttccagt ccagatttat cctctctaaa
     4561 aatctcagac atgattttaa aactagtttg gtttccatac aatttatctg ccgcttcaaa
     4621 gaacgcatca tgacttgcat attcattggg aaagttagat agaatacagg agaaagaaac
     4681 ctcttcttgc ggataaacag gtttatccgt gaaacattga actgtatcta agaaagtatt
     4741 ttttttgtct tttagattta tatagagttt gtttccagtc agagggatat ttcctgtatt
     4801 tttaagtaaa cccaaaaagc gaccaaagtt tccacttcgg attaattgga gatctttgat
     4861 ctcagctttt ggcttttctg attctggaat tggtttgaat gcgttatctc cagtagtata
     4921 gttggattgt gcaggtgttt gtttttctgc ttgtaagatg acaatactag aactccaaat
     4981 ctttcctgtt tcggtatcca caattctggc tagaattgtg gtgttgctgt tttcttttac
     5041 aatcgatcca accacaactc cttccgcagc tagtagctta cctattttga tagaatctgc
     5101 ttcagttatc ccttgactag aaagagattg ttcttttaaa actcggttga ttttagaacg
     5161 ctcaatgatt tgatattgtc cagaattcac aaattcgtga gtcaattttt cagcaaaact
     5221 gatactatca aatcctttta cacttccatc aaaaggaaga atggcgatcg tctttttttt
     5281 atattcctca ggctcagaaa ggtctcgaac tataatttta tgcggatcaa ctcctaagct
     5341 agcgcaccca tataaatgga taactagcaa aatgattgga gttagatgaa gaaataaaag
     5401 cattcttctt ttcctaaatg gattgatata gattttttgg ggttgcattg gacatactaa
     5461 aaaagtagac tcttctttca ttcaagtaga aaataggaga gactcaacat actcattcct
     5521 cacaaaaaat cggaataaaa gatttcaaaa gggaaagttt ttttaaaaag atatagaatc
     5581 ctttccttgt tagctgatct tgagagtctt tataaacttt tacactcggt agaactttag
     5641 tagatattaa tattttttta ttaggggaaa ttcgaatcca attgtattga atttattttt
     5701 ttctaaaaac tcaattcggt taaaaatttc ttgacgtgct tttttaaaat ttgtaacctt
     5761 ctgaatcttt gtccggtcag agtcggaaaa gacataaaag atgataataa aagccagtta
     5821 tatcacgaat tacggaaatt ttaggattaa aaatgaagat agccttcttg ttcaagaaga
     5881 gattatctat gaaaaaaata tggaagagcc taaaaataaa attttagaat ccaaaaaagt
     5941 aattctatgt gttgcggatg ggatgggtgg tcatttgggt ggggagttag caagccgaag
     6001 tgttctatct tttcttcgtg ataactttat gaagttatac aatgctcgca cgattaaaaa
     6061 gatattactg aaatctaaaa acattctaaa caccattgca agtgaaacga gatcatatgg
     6121 actcggaaca acggtttctg gaatgctcct caaagacaaa aaaggaatta tttttaactg
     6181 tggtgacagt cgtgtttatc gagttcagaa aatgcaacta gagcgtctaa cgattgatca
     6241 ttctgttgtg caggcaatgt ataattcagg tctcattgat gaagaggaaa tgagaacaca
     6301 tccaaataag aatctgctta catctgcgat aattggggat tattcacaga atcatccagt
     6361 aattgcaatg aaagaaattc cgatcaaaga aggagatcag ttttttattt gcacagatgg
     6421 actttgggaa agtatgagtg cagaagaact tactgaatta tttttttctt gtgaagatca
     6481 agatgagaga gcactcaagt tgaatcaact gtctcttgat aaaggtggtc gggataatat
     6541 cactatgatt ctttttgaaa ttttagaact ctaaacaaat tactcatcta taggaaattc
     6601 cttttataag atgagtgatt catataatca ttccataaaa actaaaaaaa ttttctctaa
     6661 tttattttag aagattagat cataaaattg ctacaaacac agttaatctc gcattatttc
     6721 atccttaaaa aaacaattat ggttttagag aaattcaatt tatttataat acaaataaga
     6781 aagaataagg tataataaaa gaacgaaaac ggtattgaat caatcaaaag atgaagattt
     6841 aggaggctgt atgatagaat tacttttttt attaggtttt tctctgcata atattgaaga
     6901 ggctatttgg cttccatctt ggtctaaaaa tgctgggaag tttcatcctc cggtaaaaca
     6961 aaatgagttt atgtttgcga ttgtaattgt aactatgtta ggatatttgg gaacatttca
     7021 gttttttatt tttcagggag tttcaaatat ctcgaaatct atatacttgg gatttatttt
     7081 aatgatggtt ttaaattcca tcttccctca tttagttgca acaatctatt ttaaacgtta
     7141 tgccccaggg actattactg ctttgttttt aaatctaccg atcggattgt atctaattct
     7201 tacacatctt aaaaccagac aagatttatt ctacatggta atttctgcaa taatcatgat
     7261 agttttattt atgtctggta ttgagcaaat ttttaaacta ggtgataaac tttttgaaga
     7321 gagagaatga aaatacaaaa caggaaaagc tagattcaaa aatctacttt tacttctagc
     7381 aagttttcgc tactctagaa ggatctgagt agttttgaaa gatcgaagaa tctatccaat
     7441 tagaaaattg gttagattta atttgtttat ctagtttgag agagtggaaa ttatttaata
     7501 gttagataca aatcgtttga taagaattta gttttctatc ctagttcaga aaagagaatg
     7561 aatcgaagaa ttaatttaaa aatagcttgc tatatgaaat caattcttta gagtaagtca
     7621 atttctttga gtgaaggagt tttatgatgc ttagaaaatt cgtttttatt ttactaattg
     7681 gattttcatt tctttgggca gaggattttg aatatccaac tctgatcaat ccagatggaa
     7741 ctatccaaag gcgagaattt tcaaaggagc aaaaaactac ctttgattca attcaaaaag
     7801 atgttaaata ctttcatacg gaactttcca aaattcttcc ttatttgatt cgagaaggga
     7861 aagtgaaaga gaaatcttac acaagggaga tccgaaattt tacagagact cctcctggta
     7921 gaacaaatag ccactttgtt tcagaaaatt ttgtaattaa acttacaaat gatgggaaag
     7981 tagagaatat ttctttttta aaaagaagat ctagattaaa tccaaaaatg tattctgaaa
     8041 ctgtgattcg aactatttca aatgatatca gcaatgaaga gatgactgcc ttaaaagtcc
     8101 agatcgatac ctataccaat tatacagatc catctgctcc aattcgcaaa ttagtttcta
     8161 tgtcagagat accttcacct ttggatcgga ttcgattatt aagaacttac agggcaaagc
     8221 tagaagaagc agttcgtgag atagataaac taattgacca taaagtatta aatgaccata
     8281 taaaattatc taatacgtta aacgacatag aattggaata gaaagtgaat cttgaatgga
     8341 ttctacaagc gtatattgat acagggattt ttataagtgt tttttttatt ttaaaagaga
     8401 ttttgcatct caaagtaaac cgacaacttg gattccattt ctggtatgca tttttagcat
     8461 ttatctttca agtgaagatt tctattttag taaaagaatc ttattttgta aatccctatc
     8521 tctttttatt ctctgtgagt ggtttgttta tcattggtcc ttttcagaga gcacagattt
     8581 tgaaattatt tggaaagatt caatttagag atttatacca acactatgca attctattga
     8641 ttacgattcc atttgagatt tttattattt actattatcc agatcaaatt ccagcttgcg
     8701 atcgagtaac gtatgttgga cgtgtttttt ctgctttggg tctcatttac tattttgtct
     8761 acaactatca aaatgaaaga ctatttaaaa atttcttgaa tcaatataag ttggaacata
     8821 ctaatttagt tctttcgtat aatcgaatgg tttcggtatt tgtgttgttt ggtgcagtta
     8881 gtatcttttt ctctaaaaag attctatttc aaatagatgg aatttttata tgcacgggtg
     8941 ctttt
//
LOCUS       DAOQOS010000182         8875 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_4267_length_8875_cov_6.126871, whole genome shotgun sequence.
ACCESSION   DAOQOS010000182 DAOQOS010000000
VERSION     DAOQOS010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 8875)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8875)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8875
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4267_length_8875_cov_6.126871"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..911
                     /locus_tag="PLF66_24105"
     CDS             <1..911
                     /locus_tag="PLF66_24105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020762501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HMZ67194.1"
                     /translation="KRVTIHKPQEQKLNFEDIAQRINRHEQVLTIVNRKDDANEIFKQ
                     LIGNKYYLTTNLCAEHRRDILKKVKSHLKNKEQVYLVSTQLIEAGVDIDFPIVFRAMT
                     GLDSIAQAAGRCNREGKLKDNNDKEIFGEVFLFHPEKRSPAGHLLQTEEAGKACLGDF
                     EELIHPDAFKQYFNELFWVKKEKGLDKHGIEGLKQTLYFEEIDEKFKLINEDTRSVIM
                     PYKEGKEIILTLEQMKNNKEFLDYKVIQRAQKFFVNIPIEKYGKLLNQGIISEIESVI
                     SFLNYEKFYSDSLGFIESLNAEDTIL"
     gene            889..1611
                     /gene="cas5c"
                     /locus_tag="PLF66_24110"
     CDS             889..1611
                     /gene="cas5c"
                     /locus_tag="PLF66_24110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004760107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas5c"
                     /protein_id="HMZ67195.1"
                     /translation="MQKIQYYKEIKLKQSKVFELKVWGDNACFTRPEMKAERVSYDVI
                     TPSAARAIFDAIIWKPAILWKIHRIDVLKPIRWESVRRNELGSKISLGNVTSAMKKGK
                     DNLALYIEEERQQRAGLFLRDVAYILHASFKLTNRAGNSDNVSKFEEMFLRRVEKGQC
                     HHQPYFGTREFSAYFSLNDGATKPIAIEHQDLGWMLYDLDFEEYDFDTKNYKDANPKF
                     FRAEMINGSIEIPDLNTNGARL"
     gene            1608..3356
                     /gene="cas8c"
                     /locus_tag="PLF66_24115"
     CDS             1608..3356
                     /gene="cas8c"
                     /locus_tag="PLF66_24115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas8c/Csd1"
                     /protein_id="HMZ67196.1"
                     /translation="MILKELVRYYERKLEEGEISREGFEVKEIPYLIEIDKDGNFIRF
                     IVTWLDEKKKRANKYTIPKAVVRARGINANLLWDNSEYVFGIESKDKQKDFIRKVQDL
                     KENSKEDSLESIIKFLTNYKISDLQKDENWKDIQENKPNLTFKLKGQNDIVCANEKII
                     KAISKSISDESDLEKGICLVTGEDAVIERLHAKISGVANANTVGANIVSFNLNAFESY
                     GKEQGANAPTSKYANFAYGTAINTLLSKDSHQKILIGDTTTIFWSERKSELEDEFAFL
                     FKPVAEEEEKSTQKKKGKEAEDNKKIKEHFQSPFSGKNAKLKHLLNDNTKFYILGLAP
                     NAARISIRFWYPSTVGEISKNIEQHFQDLKLEIPNNDSGFISLNRILRSTAIQGKLDN
                     INPLLAGKLITSIISGSEYPRTLLSSLINRMKAEQSITFVRVSVLKAILNRKGRNEKF
                     KNYKELQEKMDESNIDIAYRLGRLFAVMEKMQQNAINSDSTIKDRYYTSASTRPASVF
                     PVLFSLSNHHASKLGNKSIYLEKLKGEILEPLNGSIPKTFSLEEQGLFALGYYQQKQE
                     LFKKKEVNPEKEGENK"
     gene            3353..4279
                     /gene="cas7c"
                     /locus_tag="PLF66_24120"
     CDS             3353..4279
                     /gene="cas7c"
                     /locus_tag="PLF66_24120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000070174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas7/Csd2"
                     /protein_id="HMZ67197.1"
                     /translation="MSNSIQNRYDFVYLFDVKDGNPNGDPDAGNQPRVDPETGNGLIT
                     DVSLKRKVRNYITIAREAKPPFDIYIKEKAVLIDTHEKAYEAIGSKVESSKKEDKDKE
                     KRTGGEQVGKAREWMCKNFYDVRTFGAVMALKVNAGVVKGPIQFTFARSIDPVINLEH
                     SITRMAVATKAEAEAQDGDNRTMGRKHTISYGLYRAHGFISAHFAADTGFTENDLELF
                     WQALQNMWDHDRSAARGEMSCRGLYIFKHVGDINEAKLGTAPAHKLFDLVNVKRKEGV
                     DIPRNFSDYEVSIDKSKLGAGNQFSKIELIEY"
     gene            4540..5034
                     /locus_tag="PLF66_24125"
     CDS             4540..5034
                     /locus_tag="PLF66_24125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67198.1"
                     /translation="MQRNRKPTRHPSYDYSDTGYYFLTINAKEKGNVFGAVDNGSMTL
                     NELGTIAKMNWLSIPQHFLFVELDEFIIMPDHVHGIIILKDTVGNAYMRSANYDRTKM
                     RLSKIIQQYKASVTREIRKKYEIKFQWQKSFYDHIIRKEKSLYYIRKYIQQNPIRSMM
                     QTIL"
     gene            5187..5630
                     /locus_tag="PLF66_24130"
     CDS             5187..5630
                     /locus_tag="PLF66_24130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67199.1"
                     /translation="MKPKIELAPQEQLLFVRKLLPIFLDRILGHPEALVTDMTKVTHF
                     VNYDIEESIGKGSKPGHYLFKIQYKVVRKNVIALRESGEEYEDVIWDIKAIHGRKWII
                     RKCKRFFGVDITDCYYKEIPIVLKYIMMKMSRKKAIELGLIQKTK"
     gene            5667..6158
                     /locus_tag="PLF66_24135"
     CDS             5667..6158
                     /locus_tag="PLF66_24135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67200.1"
                     /translation="MGRNTKKTGNSQLMTTLFLDLEKTMIHSWDDPIIVNEERIMSSI
                     RLVNPDKVGIYSYAIYDKKDLTIFQDTIQYELEKSFQINIDPSLVFTVEEIAETIKQT
                     KNADVRKFIQEYSKQKSFYEFIKNKYRNGKFILIDDLVDNSEVFLGDLNIQFLNPFLS
                     MSY"
     gene            6640..7266
                     /gene="cas4"
                     /locus_tag="PLF66_24140"
     CDS             6640..7266
                     /gene="cas4"
                     /locus_tag="PLF66_24140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002773713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas4"
                     /protein_id="HMZ67201.1"
                     /translation="MKEWSESEIVPISAISHHLYCPRQNALIHTEGVFSDNALTTSGN
                     IGHQFVDVENSFVDHGLRKETAYRVFSEEYGISGIADIIEFPKDKPPLPIDYKNGRIS
                     SWENQESQVCAIALCLEEMLSVKIGLGAIYHVQSKRRHEFQLDQALRDKTIRAIQEIR
                     ENLVGNIVPKAEYSKRKCDNCSLFDLCLPKLERARKNDFLFKPVDFNG"
     gene            7259..8290
                     /gene="cas1c"
                     /locus_tag="PLF66_24145"
     CDS             7259..8290
                     /gene="cas1c"
                     /locus_tag="PLF66_24145"
                     /inference="COORDINATES: protein motif:HMM:TIGR03640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated endonuclease Cas1c"
                     /protein_id="HMZ67202.1"
                     /translation="MAEVKLNILYVTIEGVYIHHELNKVIVEKKGETLLEIPIHHLQG
                     ITIFGNSFLSASMLQKCLENGIYISYLTQRGKFLGRLEGAKSGNVLLRKLQFKKSDSD
                     TERIKIAKSIIAGKIQNSRLNLLRSARDLKDDDKEKEIRNAVLDMEANLKSLEDANSL
                     DSIRGYEGNAAKKYFSVFDHCIIQQKVEFEFTKRMKRPPRSRINALLSFAYSLLTNDC
                     ISACQSVGLDPFIGFLHTEKPGRPALALDLMEEFRPFADRFVLTLINRKQIQASDIIE
                     KTGSVYMMTDAARKTFLTAYQNRKQEDVTHYLLDQKCKISDLFLLQARILARAIRGEM
                     EFYIPYIWK"
     gene            8295..8585
                     /gene="cas2"
                     /locus_tag="PLF66_24150"
     CDS             8295..8585
                     /gene="cas2"
                     /locus_tag="PLF66_24150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HMZ67203.1"
                     /translation="MFILVCYDVETLTKEGKRRLRKVAKLCESHGQRVQHSVFECKMD
                     KSLYLTFENKIVSIIDTKSDSLRIYILDEDSIRKIKNFGIASVIDYEQPLVL"
BASE COUNT         3373 a         1224 c         1659 g         2619 t
ORIGIN      
        1 taaaaagggt tactattcat aaaccgcaag agcaaaaatt aaactttgaa gatattgccc
       61 aaagaataaa taggcacgag caagtattaa caatcgtaaa tcgtaaagat gacgcgaatg
      121 aaatttttaa acaattaatt ggaaacaaat actatttaac cacaaatctc tgcgcggaac
      181 atagaagaga tattctcaaa aaagtaaaaa gtcatttgaa gaataaagaa caagtttact
      241 tagtcagcac acaattgata gaagcagggg ttgacataga ttttccgatt gtctttagag
      301 caatgactgg actggacagt attgcacaag cggcaggtcg atgcaatcga gaaggaaaat
      361 tgaaagataa taatgataaa gaaatttttg gcgaagtatt tttatttcac cctgagaaga
      421 gatccccagc aggtcattta ctacaaaccg aagaagcagg gaaagcatgt ctcggtgact
      481 ttgaggaatt gatacatcca gacgcattca aacaatactt caacgaattg ttttgggtta
      541 agaaagaaaa aggattagat aaacatggaa ttgaaggatt aaaacagact ctttattttg
      601 aagagatcga tgaaaagttt aagttgatca atgaagatac aaggtctgtg attatgcctt
      661 acaaagaggg aaaagaaatt attttaacat tagaacaaat gaagaataat aaagagtttc
      721 tggattacaa agtcatccaa agagcacaaa aattctttgt aaacattcca attgaaaaat
      781 atggaaaatt attgaatcaa ggaattattt ctgaaataga aagcgtaatt tcttttttaa
      841 actatgaaaa attttacagc gactcactag gatttatcga gtcactaaat gcagaagata
      901 caatactata aggagataaa attgaaacag tcaaaagttt ttgaattaaa agtttggggg
      961 gataatgctt gttttacccg tcctgaaatg aaagcagaaa gggtttctta tgatgtaatt
     1021 acaccgtcag ccgcaagagc aatctttgat gcgataattt ggaagcccgc cattctttgg
     1081 aagattcata gaatagatgt attgaagcca attcgttggg aatcggttcg tagaaatgaa
     1141 ttaggctcta agatttctct cgggaatgtt acaagtgcta tgaagaaggg aaaggataat
     1201 ttagctttat acattgaaga ggaaaggcaa caaagagccg gactttttct tcgggacgtt
     1261 gcttatattt tacatgcttc ttttaaactt accaaccgtg cgggtaatag cgataatgtt
     1321 tccaagtttg aggagatgtt tttacgcaga gtagaaaaag gacagtgcca tcatcaacct
     1381 tatttcggaa cgagagagtt ttctgcttat ttttctttaa acgatggagc tacaaagcct
     1441 attgcgattg aacaccaaga tttaggctgg atgctttatg acttggattt tgaggagtat
     1501 gatttcgata caaagaatta caaagacgca aatcccaaat tctttcgagc agaaatgatt
     1561 aatggaagta ttgaaattcc tgatctaaat acaaacggag ccagactatg atactaaaag
     1621 aattggtaag atactatgag cggaaattgg aagagggaga aatttctcga gaaggatttg
     1681 aagtaaaaga aattccttac ttaattgaaa ttgataagga tggaaacttt attcgattca
     1741 tcgtaacatg gttagatgaa aaaaagaaga gagctaataa atatacaatt cctaaagcgg
     1801 ttgttagagc aagaggaata aatgcaaatt tactttggga caattctgaa tatgtattcg
     1861 gaatagaaag taaggataaa cagaaggatt ttattagaaa agttcaagat ttgaaagaaa
     1921 attcaaaaga agattctctc gaatctataa ttaaattttt gactaactat aagatttctg
     1981 atctacaaaa agatgaaaat tggaaagata ttcaggaaaa taaaccaaat ttaacattta
     2041 aacttaaagg acaaaatgat attgtctgtg ctaatgaaaa aatcattaaa gcgatttcta
     2101 aaagtatatc agatgaatct gatttagaaa aaggaatctg tttagtaact ggtgaagatg
     2161 ctgtaattga aagacttcat gcaaaaataa gtggagttgc gaatgctaat acagtgggag
     2221 caaatattgt ttcatttaat ttaaatgctt ttgaatctta tggtaaagaa cagggagcaa
     2281 atgcgccaac tagtaaatat gcgaactttg cctacggaac ggcaatcaat actttacttt
     2341 ccaaggatag tcatcagaaa attctaattg gagacacgac gactattttt tggtcagaaa
     2401 gaaaaagtga actcgaagat gaatttgctt ttttatttaa accagtagca gaagaggaag
     2461 aaaaatcgac ccaaaagaaa aaaggtaagg aagctgagga taataaaaaa atcaaagaac
     2521 attttcaatc tcctttttcc ggtaagaatg caaagttaaa acatttactg aatgataata
     2581 ctaagtttta tattctagga ctagcaccta acgccgcaag aatttccatt cgtttttggt
     2641 atccttccac agttggagag atttctaaaa acatagaaca acattttcaa gatttgaaat
     2701 tagaaatacc aaacaatgat tcaggattta ttagtctgaa tcgaatatta cgatcgactg
     2761 ctattcaagg aaagttagat aatatcaatc ctctgttagc tgggaaatta attacatcta
     2821 tcataagtgg aagtgaatat cctagaacat tattatctag tttaatcaat cgaatgaaag
     2881 cagaacaaag cattacattc gttagagtct cagtattaaa agcaatctta aaccgaaaag
     2941 gaagaaatga aaaatttaaa aactataagg agttacaaga aaaaatggat gaatcaaata
     3001 tagatatcgc ctatcgttta gggagattgt ttgcggttat ggaaaagatg caacaaaatg
     3061 caatcaattc agatagcaca atcaaggata gatattacac atctgcaagc acacgccctg
     3121 caagtgtttt tccagtttta ttcagtttaa gcaatcacca tgcaagcaaa ctaggaaata
     3181 aaagtattta tttggaaaaa ttaaaaggtg aaatactaga acctctcaat ggaagtattc
     3241 caaaaacatt tagcttagaa gagcaagggc tttttgcgtt aggatactat cagcaaaaac
     3301 aagaactatt taagaaaaaa gaagtgaacc cagaaaaaga aggagaaaat aaatgagcaa
     3361 ttcaattcaa aatcggtacg actttgtata tctatttgat gtaaaagatg ggaatccaaa
     3421 cggagatcca gatgcaggca atcaaccaag agtggatcca gaaacaggaa atggtttaat
     3481 taccgatgtt tcattaaaaa gaaaagttag aaattatata actatcgcaa gagaagcaaa
     3541 gcctcctttt gatatttata ttaaagaaaa agctgtattg atagacactc acgaaaaagc
     3601 atatgaagct attggttcaa aagtggagtc atccaaaaaa gaggataagg acaaagaaaa
     3661 aagaactggt ggagaacaag ttggcaaagc ccgtgaatgg atgtgtaaaa atttctatga
     3721 tgtaagaaca tttggcgcag taatggcatt gaaagtaaat gctggcgttg tcaaaggtcc
     3781 gattcaattt acttttgcga gaagtatcga tcctgtcatt aatttagaac atagtataac
     3841 tagaatggct gttgcaacca aagcagaggc agaagctcaa gatggagaca atcgcacaat
     3901 gggtcgtaag cacacaatct cttacggact ctatcgcgca catggtttta tctctgcaca
     3961 ttttgcggct gatacaggtt ttactgagaa tgatttagaa ttgttctggc aagctttaca
     4021 aaatatgtgg gatcatgatc gttctgcggc tcgcggcgaa atgtcttgtc gcggattgta
     4081 tatttttaag catgtaggcg atatcaatga agcaaaatta ggaactgctc cagcgcataa
     4141 actctttgat ttagttaacg ttaaacgaaa agaaggtgta gacattccga gaaattttag
     4201 tgattatgaa gtctccatcg acaaatctaa attaggcgca ggaaatcaat tctcaaaaat
     4261 agaattgatt gaatactaaa aacagaaaac atccccaatt tcggcaaaga ccgaattggg
     4321 gagagcagta atcagaaaaa acaattgaaa atccatttga aattatacca ttaaataact
     4381 attatttaat ataaaataaa aatatatttt aaaatcaatg ggactcatgg ggcgaagcta
     4441 tatatagtaa gaaatacatc tactaataca aggaacgcat gtaactcaat gttgcttctt
     4501 tggggcgcaa cataatgttc catacgctgg caaaaaacta tgcaacgaaa cagaaaacca
     4561 acacgacatc cctcgtatga ttactccgat acaggatatt atttcttaac aatcaacgcg
     4621 aaggaaaaag gcaatgtatt tggggcagtg gataacggta gtatgacgtt aaacgaattg
     4681 ggaactattg caaagatgaa ttggttatcg ataccacaac attttctttt cgtagaatta
     4741 gatgaattta ttattatgcc ggatcatgtt catggaataa ttattctgaa agatacagta
     4801 gggaacgcat atatgcgttc cgccaattac gatcgaacca aaatgcggct atctaaaatt
     4861 atccaacaat acaaggcatc tgtgacaagg gaaattcgaa agaaatatga aattaaattt
     4921 caatggcaaa agtcatttta tgatcatatc attcgaaaag agaagtcctt gtattatatt
     4981 cgaaaataca ttcagcaaaa tccgattagg tcaatgatgc aaacaatatt ataagggtag
     5041 ggatcgcata catgcgatcc tcataatata tacaaagtct tatttgtata aaaaatatta
     5101 aaaggattta atcgttatat aaatcaattt gtagtgtagg gaacgcatgt atgcgttccc
     5161 tacgataaat aaaaaaaata aaatctatga aacctaaaat cgaattagct ccacaagaac
     5221 aattgttgtt tgttagaaaa ttacttccta tatttttaga tagaatatta ggacatccag
     5281 aagcgttagt tacggatatg acgaaggtta ctcattttgt gaattatgat atagaagaat
     5341 caatcggtaa gggtagtaaa cccggacatt atctatttaa aatccaatac aaggtagtta
     5401 gaaaaaatgt gattgctttg cgagaatcag gagaggaata cgaagacgta atatgggata
     5461 taaaagcaat tcatggaaga aaatggatca ttcgaaagtg taaaagattt tttggtgttg
     5521 atatcacaga ttgttactat aaggaaattc ctatcgtttt gaaatatata atgatgaaga
     5581 tgtcgagaaa gaaagcaatt gaattggggt taatacaaaa aacaaaatga tatattattt
     5641 aaatcctgac gaattagagg ttactcatgg gacgaaatac taaaaaaact ggaaactcac
     5701 aactgatgac tactttattt ttagatctag agaaaacaat gattcattca tgggatgatc
     5761 caatcatagt caatgaggaa aggataatga gtagtatccg cttagtcaat cctgataaag
     5821 ttggaattta ttcttatgct atttatgata aaaaagattt aacaatattt caagatacaa
     5881 tccaatacga actagaaaaa tcgtttcaga taaatataga cccatcgtta gtctttacag
     5941 ttgaagaaat agcagaaaca atcaagcaaa ctaaaaatgc agatgtaaga aaatttatcc
     6001 aagagtatag taaacaaaaa tccttttacg aatttattaa aaataaatat cgtaatggaa
     6061 aattcatttt aattgatgat ttagttgata acagtgaagt tttcctagga gatttaaata
     6121 ttcagttttt aaatcccttt ttatcaatgt catattaata gagaaatacg tagggatcgc
     6181 atacatgcga tccgtgtgat atatataaag tcttgtttgc ataaaaatat caataggatt
     6241 caatcgttat atgaatcaat ttgtagatgc agggaacgca tttatgcgtt ccttacaata
     6301 tatataagca caatttttaa ctgtaacgtt aacttgtaac tctgatagga tagatttaat
     6361 tcatataacc ctaattgaaa atcaaaagta aaatatttta ttattatgct ctttgaaatt
     6421 gaaaataatc cgtatcataa taaattaaaa gaattttatg ttactgatta atagatgata
     6481 tatcctctaa aaatctaaat catttggatt gtttcttgtt tccttttaca ttcttaattc
     6541 taaacaaagt atttagtgga gtcattcatc aaaaattgga aattacatga agaatctatc
     6601 tagtcagata tatttacaaa atttttataa aaggttttta tgaaagaatg gtctgaatca
     6661 gaaattgttc ctatttcggc aattagtcat catttgtatt gtccgaggca aaacgctctt
     6721 atccacacag agggagtatt tagtgataat gcgcttacta cttctggaaa tattggtcat
     6781 cagtttgtag atgtggaaaa tagttttgta gatcatggtt tgcgcaaaga gactgcgtat
     6841 agagttttca gcgaagagta tggaatctca gggatagcgg atataatcga atttccaaaa
     6901 gataaaccac ctcttcctat tgattataaa aatggaagaa tctcgtcttg ggagaatcaa
     6961 gaatctcaag tatgtgcaat cgctttatgt ttagaagaaa tgctttctgt aaaaattggt
     7021 ttaggtgcga tctatcatgt tcaatctaag aggagacatg aatttcaatt agatcaagcg
     7081 ttaagagata aaacgattcg agctatacaa gaaataagag aaaatttagt tggaaatata
     7141 gttccaaagg ctgagtattc gaaaagaaaa tgtgacaatt gtagtttatt tgatctttgt
     7201 cttcctaaat tagaaagggc aaggaagaat gattttttat ttaaaccggt agactttaat
     7261 ggctgaagta aaattaaata ttttatatgt tactattgag ggtgtttata ttcatcatga
     7321 attaaacaaa gtaatagtag agaaaaaagg ggaaactctt ttagaaattc caattcatca
     7381 cttacagggt ataacgattt ttggaaatag ttttttaagt gcgtccatgc tccagaaatg
     7441 cttagaaaat ggaatttata tttcttattt aacccagagg ggtaaattct taggaaggct
     7501 ggaaggtgct aaatctggaa atgttttact caggaaactt caatttaaaa agagcgattc
     7561 ggatacagaa agaatcaaaa tagcaaaatc gattatcgcc ggaaagattc aaaactcaag
     7621 attaaatctc cttagaagtg cgagggattt gaaagatgat gataaagaaa aagaaattag
     7681 aaatgcagtt ttagacatgg aagcaaatct aaaaagttta gaagatgcaa attcattgga
     7741 tagtattaga ggctatgagg gaaacgcagc taaaaaatat ttttcagtct ttgatcattg
     7801 tatcattcaa caaaaggtag agtttgaatt tacaaaaaga atgaaacgtc ctccaaggag
     7861 tagaatcaat gcgttactct cctttgctta ttctttgctt acgaatgatt gtatttctgc
     7921 ttgtcaatct gtcggtcttg atccttttat tggattttta catacagaga aacccggtcg
     7981 acctgcactt gctttagatt tgatggaaga gtttagaccc tttgcggata gatttgtatt
     8041 gacgttaatc aataggaaac aaatacaagc gagtgacatt attgaaaaga ctggttctgt
     8101 ttatatgatg acagatgctg ctagaaaaac ttttcttact gcatatcaga atagaaagca
     8161 agaagatgtt acgcattatc tactagatca aaaatgtaaa atctcagatt tatttctttt
     8221 gcaggctaga attttagcac gagcaataag gggagagatg gagttttata taccttatat
     8281 atggaagtaa taaaatgttt atattagtct gctatgatgt agaaacctta acaaaagaag
     8341 gaaaacgaag attgcgaaag gtcgctaagc tttgcgaaag tcatggtcaa agagttcagc
     8401 actctgtctt cgaatgcaaa atggataagt ctttgtattt aactttcgaa aataaaattg
     8461 tatccatcat tgatacaaaa tcggatagtc ttagaattta tatacttgac gaagattcta
     8521 tacgaaagat aaagaatttc ggaatagcct ctgtaattga ttatgaacaa cctcttgtgt
     8581 tataaaaata tgcgaaccta tatctcccta aaaaaagggg ttggttcgca gaataaagaa
     8641 aatacttata tagaaagact caaattgact tttggtagat tcgtattttc gaatttcgaa
     8701 aatgtaaaag cattgttcgc gaaatttttt ttaaacattc gttatatggc tatcctatgc
     8761 gaaaattaca agcggagaaa tctgctattt ctctcttctt aattgaagag agtggattga
     8821 aacataaaga tagacaagca cgcattaacc ccacttactt atttctctct tctta
//
LOCUS       DAOQOS010000183         8654 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_4434_length_8654_cov_7.363182, whole genome shotgun sequence.
ACCESSION   DAOQOS010000183 DAOQOS010000000
VERSION     DAOQOS010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 8654)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8654)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8654
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4434_length_8654_cov_7.363182"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..394
                     /locus_tag="PLF66_24155"
     CDS             <1..394
                     /locus_tag="PLF66_24155"
                     /inference="COORDINATES: protein motif:HMM:NF016847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HMZ67204.1"
                     /translation="EKSFQDQTIAIYEFMAKMLFFLPDPNQKMIRYYGIYSNRIMVRH
                     ACLDAAAHQPDNSVLSQRTWAQAIENSFDAKPQHCPDCTKNPLIPLQRRIMRLTVVYS
                     YSARKTIEGLKETHTYMNGYFRPKSRAP"
     gene            599..1177
                     /locus_tag="PLF66_24160"
     CDS             599..1177
                     /locus_tag="PLF66_24160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67205.1"
                     /translation="MGDDYGGSSMQIPRKKIDKLSIDAPNDPTLNYYQTEIWYSKGEQ
                     EYSAGNFKKALGYYEKAYQYWSANPLLVERIKDVKEKLKNPKRSNLENSKTSLDEINN
                     ISDEQVSTSVSINSRAIDSLSLKNRNVNLIILDKELQEQIREQFSKINDVNKEKELIV
                     IPKSYLILFLILGGSLVTANLVGLYILIRKSE"
     gene            1174..2370
                     /locus_tag="PLF66_24165"
     CDS             1174..2370
                     /locus_tag="PLF66_24165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67206.1"
                     /translation="MNIRIFLISLFLFNFMYLSPDRKHWIDLVYERFPVVKYKVNPLM
                     IGFNTGSKESIIDAIRSSSDFWSFRSGDAHFQFSYNGTTTNSPLAFNGIACDEDGKQR
                     IRETDNLVFASNTDDPDCSGQACTYIWSCTDKNEILHFDMQINSREYEWDTGTNHKKS
                     FNLTTVAAKQFGQILGLDHCPLGLNTESCSSEVASRGTSDPSFDSLLYRFIDAEVIRN
                     SLSSDDRAGLQSLYGSISPEEATMKSEMNEFYSLADRICNPSPCVIPEEDIGKYQLST
                     EEIQARAYHISELAKDGLDYRTAIFEMTQMLKKLHQDSYIETGNSAETYLLSVTKEMI
                     QDISGLSPEMLLKSRQILSIQIKNRKFILSQFKNEFDTDFLKFTQAELNLLIQYRREI
                     INRMGQ"
     gene            2373..2684
                     /locus_tag="PLF66_24170"
     CDS             2373..2684
                     /locus_tag="PLF66_24170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67207.1"
                     /translation="MRLILFILFTLFLFQCNTNDPFNDPTGEKTKKVKKKKSCNRDAL
                     VVYLIFNNDYRFSCNNPDYVKGINAKSLEECLSKRNESIIFNLGTVYSVCSSDEEGAN
                     G"
     gene            2677..3945
                     /locus_tag="PLF66_24175"
     CDS             2677..3945
                     /locus_tag="PLF66_24175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67208.1"
                     /translation="MVKIKLIILFFLLSTLLYAFGAGVTTPPSCGFFSWNIPDEIPLG
                     TANDLPQFTVFGDSRAKFVSEHGRLGNFADATKDGKKIIAGNQIKKIQNLGWAGATAS
                     DNQRDLFEWISGYLSQTPGKYKEMTWWRQWDRCKQSGVSYVTHPKTVMNLGGNDMVNF
                     EKYYATRDGLLGPLTVILNSEALLGMFGKPASPFEKEMRWSWAFNSKEEIILRNMKST
                     ASRILNQSAHHQLLIVDIAPRGPSIALGDNLTDLGRVARLNVYISRLNRKYYDRLIPE
                     LQSYHGQYRVHFLDVFNRFYANLIGEWATFYYGGSQPSSFLPLDGIHYGNEGNREFAL
                     MITAKMLRIGWFQRDPNITDAMLDDISAGIPIDLSGVCDLKCWATICYLTKVCKFTDL
                     LKMYFQDQLDQQFWQDQEEYERQQQQNQNP"
     gene            3967..4761
                     /locus_tag="PLF66_24180"
     CDS             3967..4761
                     /locus_tag="PLF66_24180"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HMZ67209.1"
                     /translation="MISKNIKLSNDELMDINHKWKRRKRIHRPRTLIFFGTFFLILPL
                     INYIGIAIRFKHRLVEFSNIFSELHSLEIILLCIPIFVGIGILMVQTWGYYLFIFYAS
                     ILTIHNTITLFRDPAIYNYNSLLQTILIFGAIYYFLKKEVSAPYLFRPNEDIVISNIL
                     ENYRSILYIPNGWRRSVRKQIEITVNVSNHSLVTKNFSLSGFYADWVDPALNPSEEVQ
                     LEFNIRNQKYSLKGGVVRVDENGVGIAFRNMTKEVKRSLQLSLNSL"
     gene            4810..5187
                     /locus_tag="PLF66_24185"
     CDS             4810..5187
                     /locus_tag="PLF66_24185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67210.1"
                     /translation="MKIIVKKIRINVIILILVMVSCVGVKSKKGVSTEFILFASDINK
                     TDQQSGVTQASPGADNSPAGSGVTPGNNSGGGFIDTTLTIINGLFGGGGQVTSIPADK
                     VILRTKLFDDPVLDAANKSCPTF"
     gene            5332..8445
                     /locus_tag="PLF66_24190"
     CDS             5332..8445
                     /locus_tag="PLF66_24190"
                     /inference="COORDINATES: protein motif:HMM:NF037202.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HMZ67211.1"
                     /translation="MPINNYLEKINRLYKTGNAREHSYRGDLQVLINTIVPDVLVTNE
                     PARVECGAPDYIITKRDIPVGYIEAKDIGVDLESKSLKEQFDRYKASLQNLIFTDYLT
                     FHLYINGEFKTKIDIAELKNGKIVPIQNNFSTFIDLIKDFCLFEGQTIKSSSTLAAMM
                     AKKARMLENIIEAALVSDIKKEETSSLRDQMDAFQKILIHDITPKEFADIYAQTIAYG
                     MFAARFHDKTLETFSRQEAAELIPKSNPFLRNLFSYIAGPNIDDRIKWIVESLAEIFR
                     SADVASLLKDFGKATQTQDPIIHFYETFLTEYDPALRKARGVWYTPSPAVNFIVRAID
                     DILKTEFGIREGIADNSIIDIEVKQPSEKKLAKKNVHRVQFLDPATGTGTFIAELIRQ
                     VYQKFQGQEGIWSNYVENHLIPRLHGFEILMASYAMAHLKIDMLLQETGFKATKSQRL
                     KIYLTNSLEEYHEDDGTLFALARWLSEEAHQANDVKRDTPVMVVMGNPPYAVSSSNKS
                     DWIQELIADYKKDLNEKNIQPLSDDYIKFIRFGQHFIDKTGEGILAYISNNSFIDGLI
                     HRQMRKHLLESFDKIYILDLHGNAKKKEVSPDGSVDQNVFDIMQGVSINLFVKTNKQK
                     KKDLAKVFHYDLFGKRELKYDFLNENNIKKIEWNNLEPYEPEFFLVKKDFGIENENIY
                     FKIESLFEVYNSGLTTEFDELAIQDTFESGKLLLDDLKTKTSNDIEKKYQLGITKLEK
                     ISNAIKDVNSRVATVKNIYYRPFDRRYTIYTGKTNGIMGRPRNDLMKHFFKDNLGLVI
                     GRQGQAVGSMEWNVLTITDTIVDCNIFYRGRGTVFPLYLYPESANPLLSATDTRQPNL
                     NMNIINEIAGKMGLTFTSEKETTNNSFSPIDILDYIYAVLHSPTYREKYKEFLKIDFP
                     RVPYPEDKKTFWKLVKLGEELRQIHLLESDVVNTYKTSYPVSGDNIVDKIKFDNGNVY
                     INETQYFSKVPEVAWNFYIGGYQPAQKWLKDRKERMLGFEDIKHYQKIIVALIETDRI
                     MKEIDKI"
BASE COUNT         3080 a         1358 c         1633 g         2583 t
ORIGIN      
        1 agagaaaagc tttcaggatc aaacgattgc tatttatgag ttcatggcga agatgctttt
       61 ttttcttccc gatccaaatc aaaagatgat acgctactac ggaatctatt ccaatcgcat
      121 tatggttcga cacgcatgtt tggatgctgc tgctcaccaa ccggataatt ccgtattgtc
      181 acaacgaact tgggcgcagg ccattgagaa tagttttgat gcaaaaccgc agcattgtcc
      241 ggattgcaca aaaaatcccc taatcccctt gcaaaggagg ataatgcgac taacggttgt
      301 ttattcctac tcagccagaa aaaccattga aggattaaaa gaaacgcata cttatatgaa
      361 tggatacttt cgtccaaagt ctagagcacc ttgataaatg agaaatgttg aaaaccaaaa
      421 attgattcct attgacaatt tttaaagcca ggtttttctt ggatactaga ggaatgcctt
      481 catgaccttt gagcttttaa attcgggcaa aaaattttac cccgtttagt tcaaaacatc
      541 tctttatgaa agactcgcaa tccttttttg ctttaagcct ttaacttgta agagagaaat
      601 gggagacgat tatggaggaa gttcgatgca aattcctaga aagaaaatag ataagttatc
      661 aatagatgca ccgaatgatc caactctaaa ttactatcaa acagagatat ggtattcaaa
      721 gggagaacaa gaatatagtg caggtaattt caagaaggct ttaggatact atgaaaaagc
      781 ctatcaatat tggtctgcaa atccgttatt ggttgaaaga attaaagatg taaaggaaaa
      841 attgaaaaat cctaaacgtt caaatttgga aaattcaaaa acctcccttg atgaaattaa
      901 taatattagt gatgaacaag tctctacctc tgtctcaata aatagtagag cgatagactc
      961 tctatctttg aaaaatagaa atgtcaactt aataatattg gacaaagaat tgcaggaaca
     1021 gattcgggag cagttttcaa aaataaacga tgtaaataaa gagaaagagt taatcgtaat
     1081 acccaaatcg tatctaattc tttttttaat attaggtgga agtttagtaa ctgcaaacct
     1141 tgtcgggtta tatatcctaa taaggaaaag cgaatgaaca ttcgtatttt tttaattagt
     1201 ttatttttat ttaactttat gtatctatca cctgatagga agcactggat agatttagtc
     1261 tatgaacgat ttccagttgt gaagtataaa gtaaatcctt taatgatcgg gttcaatact
     1321 ggaagtaaag aaagtattat tgatgcaata cggagttcct cggatttttg gtcgtttcgt
     1381 tcaggagatg cacattttca attttcttac aatggaacaa caacaaattc tcctttagca
     1441 tttaatggaa tagcgtgtga cgaggatggg aaacagagaa ttcgagaaac ggataattta
     1501 gtatttgcta gtaataccga tgatccagat tgttccggtc aggcttgcac atatatttgg
     1561 agttgcactg acaaaaatga aatacttcac tttgatatgc aaatcaattc aagagaatat
     1621 gagtgggata cagggacgaa tcataaaaaa agttttaatt taactacggt agcagcaaaa
     1681 caatttggac aaatactcgg tttagatcat tgcccattag gattaaatac ggaatcttgt
     1741 tcgagtgaag ttgcatctag aggaacgagt gatccttctt ttgattccct attgtatcgg
     1801 tttatagacg cagaagtgat tcgaaatagt ttaagttcgg atgatagggc aggattacag
     1861 tcgttatacg gtagtatatc accggaagaa gccacaatga aatcagagat gaatgaattc
     1921 tattccttag cggatcgtat ttgtaatccg agtccctgtg ttattcctga agaagatatt
     1981 ggtaaatatc aattatccac agaggagata caggctagag catatcatat tagtgaacta
     2041 gcgaaagatg gtttagatta tagaactgca atatttgaaa tgacgcaaat gttgaaaaag
     2101 ttacaccaag attcatacat agagacaggt aatagtgcag aaacctactt gttgtccgta
     2161 actaaagaaa tgattcaaga tatttcagga ttatctccag aaatgctttt gaaaagtagg
     2221 caaatacttt ctatccaaat taaaaatcga aaattcattt taagccaatt caaaaatgaa
     2281 ttcgatactg attttttaaa attcacacaa gcggaactga atctcctaat tcaatacaga
     2341 agagaaatca taaataggat gggacaatga ctatgcggct aatattattt attcttttta
     2401 cgttattttt atttcaatgt aatacaaatg atcctttcaa tgatcccaca ggtgaaaaaa
     2461 caaagaaggt aaaaaagaag aaatcctgca atcgagatgc tttagtagtt tatctaattt
     2521 ttaataatga ctatcgcttt agttgtaata atcctgatta tgtaaagggt ataaatgcaa
     2581 aatctttaga agaatgtttg tcaaaaagaa atgaaagtat aatatttaat ttaggcactg
     2641 tatatagtgt atgttcttca gatgaggagg gcgcgaatgg ttaaaataaa attaataata
     2701 ctcttctttc tcttgtctac tctgttatat gcatttggag cgggtgttac cacgcctccc
     2761 tcctgcggtt tttttagttg gaacattccc gatgaaattc ctttaggaac agcaaatgat
     2821 ctgccacaat tcactgtatt tggtgacagt agggcaaagt ttgtaagtga acatggtagg
     2881 ctaggtaatt ttgccgacgc aactaaagat gggaaaaaaa tcatagcagg aaatcaaatt
     2941 aagaaaattc aaaatttagg ttgggcaggt gcaacagcct ctgacaatca aagagattta
     3001 tttgaatgga tttctggcta tttgtctcaa actcccggta aatataaaga aatgacttgg
     3061 tggagacaat gggataggtg caaacaaagc ggggtctctt atgttaccca tccaaaaact
     3121 gtaatgaact tgggtggaaa tgatatggtc aattttgaaa aatactatgc tactcgcgat
     3181 ggactattag gtcctttaac tgtaatttta aattctgagg cacttttggg aatgtttgga
     3241 aagccagcct ctccttttga aaaagaaatg agatggagtt gggcatttaa ttctaaagaa
     3301 gaaattattt taagaaatat gaaaagcacg gcgagtcgta ttttaaatca atccgctcac
     3361 caccaattac taattgtaga tatagcacct cgtggaccga gtattgcgtt aggcgataat
     3421 ttaacggatt tgggaagagt tgctaggtta aatgtataca tttccagact taatcgaaaa
     3481 tactacgatc gtttaatccc agaattgcaa tcttaccatg ggcagtatcg cgttcatttt
     3541 ttagatgtct ttaatcgttt ttatgcaaac ttaattgggg agtgggctac cttctattat
     3601 ggtggttcac aacccagctc ttttttacct ttggacggca tccattatgg aaatgagggt
     3661 aatcgtgagt ttgcgttaat gattacagct aaaatgcttc gtataggctg gtttcaaaga
     3721 gatccaaaca taacggatgc aatgttggat gatatatcgg ctggtatccc aatcgacctc
     3781 tccggtgtct gcgatttgaa atgttgggct actatctgtt atctcacaaa agtttgtaaa
     3841 tttacagatt tactaaagat gtatttccaa gaccaattag atcagcagtt ttggcaggat
     3901 caagaagaat atgaacggca acagcaacaa aaccaaaatc cataaaataa tgtataagga
     3961 gagatgatga taagtaaaaa tataaaattg tcaaatgatg agctaatgga tataaaccac
     4021 aagtggaaaa gaagaaaaag aattcacaga cccagaacat taattttctt tggaactttc
     4081 tttcttattc ttccattaat aaattacatt ggtattgcca ttcgatttaa gcataggctt
     4141 gttgagtttt caaatatttt tagtgaatta cattctttag aaataatact actatgtatt
     4201 ccgatttttg ttggcattgg aatattaatg gttcagactt ggggttacta tctatttatt
     4261 ttctatgctt ctatcttaac catacacaat acaataacat tatttcgtga tccagcgata
     4321 tacaactata attcattact tcaaactatt ctaatttttg gagctatcta ctactttctt
     4381 aaaaaagaag tctcggcacc ctatcttttt agacctaatg aggatattgt gatttctaat
     4441 atattagaaa actatcgttc cattttatat atcccaaatg gttggaggag aagtgttaga
     4501 aaacaaatcg aaataacagt aaatgtttct aatcacagtc ttgtaacaaa aaactttagt
     4561 ctctctggtt tttatgcaga ttgggtagat cccgctttga atcccagcga agaagttcaa
     4621 ttagaattta atattcgaaa ccagaaatat tctcttaagg gaggagtcgt gcgtgtagat
     4681 gaaaatggag ttggtatagc ttttagaaat atgacgaagg aagttaaaag gtcactgcaa
     4741 ttgagcctaa actcgcttta aaacaagagt gattataaat aatgctaata atgaataggg
     4801 aaaaaatgaa tgaaaataat agtaaaaaag attagaataa acgttattat actcatccta
     4861 gtgatggtat cttgcgttgg agtgaaatca aagaaaggtg taagcacaga atttatccta
     4921 tttgcatccg atattaataa aacagatcaa caatctggtg ttacacaggc ttctcctgga
     4981 gcagataatt cgcctgctgg ctctggagta actccaggaa ataactcagg tggtggattt
     5041 attgatacaa cacttacaat aataaatgga ttgttcggag gagggggaca agtaacaagt
     5101 attcctgctg ataaagtaat tcttcgaaca aaactattcg atgatcctgt tttagatgcg
     5161 gctaataaaa gttgtccgac attttagcct cagccctgaa tcgaacgagg attgatgctt
     5221 gggactttaa gatataggtt tcctcgacct cgtgtctcgg aaagactcta gaaaaatata
     5281 tttgacttta gagtatgtac attaagtatt cattgaaaca aaggatagct aatgccaatc
     5341 aataattatt tagaaaagat aaatagactc tacaaaacag gaaatgcaag agagcattct
     5401 taccgtggag acttacaggt attaatcaat actattgttc cagatgtttt agttacaaat
     5461 gaaccagctc gggttgaatg cggtgcacct gattatatta ttacgaaaag agatattccg
     5521 gttggttata ttgaagcaaa agatattgga gtagatttag aaagtaaatc actaaaagaa
     5581 caatttgata gatataaagc atctttacaa aatttaattt ttacagatta cctaactttt
     5641 catctttata taaatggcga atttaaaaca aagattgata ttgctgaatt aaaaaatgga
     5701 aaaatagttc caatccaaaa taatttttcg actttcatag acctcataaa agacttttgt
     5761 ttatttgaag ggcaaacaat aaaatcctct tcaacattag cagctatgat ggcaaagaaa
     5821 gctaggatgt tagaaaacat aattgaagct gcactagttt cagatattaa gaaagaagaa
     5881 acaagttctc tgagagatca aatggatgcg tttcaaaaaa tactaatcca tgacataaca
     5941 ccaaaagaat ttgctgacat atatgcccag acaattgcct acggaatgtt tgccgcaagg
     6001 tttcatgaca agacattgga aactttcagc aggcaagaag cggcagaatt aatccctaaa
     6061 tcgaatccat ttcttagaaa tttattcagt tacattgcgg gtccaaatat tgatgaccgg
     6121 ataaaatgga ttgtggaatc gttagctgaa atttttcgtt cggcagatgt tgcgtccctc
     6181 ttaaaagatt ttggaaaggc aacacaaaca caagacccta tcattcactt ctatgaaaca
     6241 tttctaacag agtatgaccc tgcccttcgt aaggctagag gagtttggta tactccaagt
     6301 cctgctgtga actttattgt gcgtgcaata gacgatatac taaagactga gtttggaatc
     6361 agagaaggaa ttgccgataa ttcgattatt gacatagaag taaaacaacc cagcgaaaag
     6421 aaactcgcaa agaaaaacgt ccatcgagtt caattcctag accctgctac aggaacagga
     6481 acatttattg ctgaactcat ccgtcaagta taccaaaaat tccaaggaca agaaggcatt
     6541 tggagtaatt atgtggagaa tcatcttata ccgcgactgc atgggtttga aattttaatg
     6601 gcttcttatg caatggcaca tttgaaaata gacatgctct tgcaagagac tggctttaag
     6661 gcaaccaaaa gccagcggct caaaatctat cttaccaact ccctcgaaga ataccacgaa
     6721 gacgatggaa ccttattcgc attagcccgt tggctctcgg aagaagcaca tcaggcaaac
     6781 gatgttaaac gcgatacacc tgtaatggtt gtaatgggta atccaccgta tgcggtcagc
     6841 agtagcaata aaagcgattg gattcaggaa ttaattgcag actataaaaa agatttgaat
     6901 gaaaaaaaca tccaaccact ttcggatgat tatatcaagt tcattcgttt tggtcagcat
     6961 tttattgata aaacagggga aggcatattg gcgtatatct ctaataacag tttcattgat
     7021 ggacttattc atcgtcaaat gcgtaaacac ttactagaaa gctttgataa aatttacatc
     7081 ctcgacctac acgggaacgc taagaaaaaa gaggtttcac cagatggttc tgtagaccaa
     7141 aatgtattcg atattatgca aggtgtgtcc attaacctct tcgtgaaaac aaacaaacaa
     7201 aagaaaaagg atcttgcaaa agtatttcac tacgacctat tcggaaaacg agaattgaaa
     7261 tacgatttct taaatgaaaa taatattaaa aagattgagt ggaataattt ggagccatat
     7321 gaaccggagt tctttttggt aaaaaaagat tttggtatag aaaatgagaa catttatttt
     7381 aaaattgaat cattatttga agtctacaac tcggggctaa cgacagaatt cgatgagtta
     7441 gcaatacaag atacatttga aagtggtaaa ttactattag atgatttaaa aacaaaaacc
     7501 agtaatgata ttgaaaaaaa ataccaactt ggaataacga aattagaaaa aattagcaac
     7561 gcgataaagg atgttaattc aagagtagca actgttaaaa atatttacta cagaccattt
     7621 gaccgcaggt ataccatata tactggcaaa acaaatggaa ttatgggaag acctcgcaat
     7681 gatttaatga aacatttttt taaagataat ttaggtttag taattgggcg gcaagggcaa
     7741 gctgttggaa gcatggaatg gaatgtatta actattacag atacaattgt agattgtaat
     7801 attttttata gaggacgtgg aacggtcttc cctctctatc tctacccaga atccgccaat
     7861 ccgttgttat ctgcaactga cactaggcaa ccgaatttga atatgaatat aataaacgag
     7921 attgcgggta aaatgggatt aacctttact tcggaaaaag aaactacaaa taattccttt
     7981 tctccaattg atattttaga ttatatctac gccgtattgc attcgccgac atacagagaa
     8041 aaatacaagg aatttctaaa aatagatttt ccgagagtgc cttatccaga agataaaaaa
     8101 actttttgga aattagtaaa attaggagag gagttacgac agattcactt attagaatca
     8161 gatgtggtta atacatataa aacgtcttac cctgtttcag gagataatat tgttgataaa
     8221 ataaaatttg ataatgggaa tgtttacatt aacgaaactc aatacttttc taaagttccc
     8281 gaagtagcat ggaattttta tatcggtggc tatcagcccg cacaaaaatg gctgaaagat
     8341 agaaaagaaa gaatgcttgg atttgaagac ataaaacatt atcagaaaat cattgtagca
     8401 ctaattgaaa cagatagaat aatgaaagag atagataaaa tttaaaaaaa gaaataattt
     8461 aaaagcaaat attgcattac gcgcctaacg ttgagtttgc ggaaattgct acagaatagt
     8521 atttgacaag caacttcgca aacccaacat attgaaaaga tttagtaatt gtgtagcgga
     8581 gtaagtatta cactcgacct cgtgtcgagg ctggttttag taaaccgcat caatagactt
     8641 catcgttcta ggga
//
LOCUS       DAOQOS010000184         8642 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_4443_length_8642_cov_6.333295, whole genome shotgun sequence.
ACCESSION   DAOQOS010000184 DAOQOS010000000
VERSION     DAOQOS010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 8642)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8642)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8642
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4443_length_8642_cov_6.333295"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..554)
                     /locus_tag="PLF66_24195"
     CDS             complement(<1..554)
                     /locus_tag="PLF66_24195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67212.1"
                     /translation="MEIVLITNLIVMIIFGLGGFFLAKFKYQSETGLSKEEILSLQNN
                     LAILQTKLDQFSKTLSEKEELLKTKEQENKYLGQNLAVRESELRASLEKSNELSLKME
                     AQNAEYKSLQTKSIELEKNLAAKMIELKSMEEFSQKQKEEIERINLKFQDDFNKIANQ
                     ILLNNSSSFNKQTSETLDNILKPLR"
     gene            735..1661
                     /locus_tag="PLF66_24200"
     CDS             735..1661
                     /locus_tag="PLF66_24200"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002976866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HMZ67213.1"
                     /translation="METQIQTKRTLGSSISLIAFILIASTVLFVFTVPFTWELFWVAF
                     FSYFIRMFAITGVYHRYFSHNAYKTSRVMQFLLAWLGCMAQQKGPLWWAAHHRNHHKY
                     SDTEKDLHSPRLTGFWNSHMLWFLKNDFNDYDPKIIKDYTKYPEIVWLDKYHWIPPLL
                     FSIVLGMISFPVLVYGYAVATFLLGHGTWTINSLSHVIGKPRFDAGDDSKNNWFLAII
                     TMGEGWHNNHHYYRHSANQGFYWYEYDFTYYILKLMSFVGLVWDLKKPPVHVLEEGMK
                     LDAMRKGKKEFKNLSGQWVPVTVRIPTAQTQA"
     gene            1895..2407
                     /locus_tag="PLF66_24205"
     CDS             1895..2407
                     /locus_tag="PLF66_24205"
                     /inference="COORDINATES: protein motif:HMM:NF019235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1564 family protein"
                     /protein_id="HMZ67214.1"
                     /translation="MKNSISKTKFFSQTNNLDLAEFSSTVLIPIQYFDLFSRKIQKHK
                     GIRSYIHYLLIKYKLHIANGLVPSYSKVTTKYQEKGQELQKVAFRPKPEDWAELKLYR
                     ISFGMSISAFLVYLLIADSVDFAETLSDYLTGVGITTTPNFNLTAKVYLWNERTYYAT
                     IFQYRNIPYH"
     gene            complement(2440..3423)
                     /locus_tag="PLF66_24210"
     CDS             complement(2440..3423)
                     /locus_tag="PLF66_24210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010572301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HMZ67215.1"
                     /translation="MYLKFFLILSLGVFLNCFLAAPLKKESKHWQPKMNPKKVFKDWN
                     DVFKSAKPIQITALKNGDVYTGPSVLIDKENSNTPEKEKIDQWVPAMSYLVSHPSEGY
                     FLFDTGVPTADKDGRCDFSLYGFIYKTACKVEPATDIASLLSKLAIQNSDLNFAIISH
                     LHGDHLGGVRDLLKRGNLNVLISEDEKKSASEAFRILHGYPMEPLSLNFNLLTLANAD
                     YVNMPKLGRVYDLFGDGSIWLIDASGHTNGELAALVVSSKGSFLFTFDSSHLKAGFEN
                     NVVPGFTVDKTKASNALSRMRNFVKEFPQVKVIYGHEPSQWSKDVIQPLTK"
     gene            3510..4184
                     /locus_tag="PLF66_24215"
     CDS             3510..4184
                     /locus_tag="PLF66_24215"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HMZ67216.1"
                     /translation="MEDYKNILQNNIFSSLNKNILKKIFQEGEIVTYKTGKIIYSAET
                     SDKIGLILKGLFRVFIYSANGRQLTIRYAQEGELLGIVSSIKTGTDVGVQSLETSTIL
                     IFSIKTLKSISIQYPELAWKIAEECSTRVYDLIKEFHRLAFRNVRQRLAFHLLSISKS
                     NSIKPYLSIGISQQDLADASGSVREVIVRELRHFKKQGFIQSKGKQIEILNPKALLEE
                     SKVDIE"
     gene            4201..4890
                     /locus_tag="PLF66_24220"
     CDS             4201..4890
                     /locus_tag="PLF66_24220"
                     /inference="COORDINATES: protein motif:HMM:NF025687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4386 domain-containing protein"
                     /protein_id="HMZ67217.1"
                     /translation="MEFEKWNKRNIAFASGFSLVGMAITAGFSFGYAINQIFVSGNAE
                     LTFNNIIQHNLLFRLGILGLLITALLDILVSGLFYLYFKETNRNISLLTALFRICYSS
                     VFIFAILQFCFVLILTKDSDYLSSLPKEKIILFIMEGFNQIFAICLMIFAFHLLGLGY
                     LVLISKEIPNILGILLILASFCYLGTSTGSLLIADYGRYKSTIDSIVALPSAIGELAI
                     AFWLIWKGGKV"
     gene            5146..5586
                     /locus_tag="PLF66_24225"
     CDS             5146..5586
                     /locus_tag="PLF66_24225"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HMZ67218.1"
                     /translation="METIEINDEFSFPPENVWSLLTDHGKYATYAPLKESSLVKEGKI
                     DRNGLGAQRKLVTDKIIFLEDITHFEPFLRFDYLIVDCSVPMKHYGGIIRFEKVERGT
                     KIHWTSSYDMLGDTSPALLRAIKSSSTKMFKEILSGVKRELVKN"
     gene            5607..6179
                     /locus_tag="PLF66_24230"
     CDS             5607..6179
                     /locus_tag="PLF66_24230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67219.1"
                     /translation="MNLDSINKNIYYLNLKELKLLCDIHKIPYKILYKSRDGKIKNTG
                     ILDRKEKIIQRILLFLKERKIGKPTLFPQNVILQTEITNLTPKEKIYFGLYKNGNKEI
                     LELLKSLTESKFKFGATAQLILLDSWAKGKLLTFQEFSQIWLKESQKKISHPEWAYLS
                     DLANGNVKDWKNKRSEIAKEVIESIKKQSF"
     gene            complement(6160..6765)
                     /locus_tag="PLF66_24235"
     CDS             complement(6160..6765)
                     /locus_tag="PLF66_24235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008593729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate-dependent dioxygenase AlkB"
                     /protein_id="HMZ67220.1"
                     /translation="MNLFPADNLENILPKDGIVNYYGKIFSDSEANQYLTQLLQTIHW
                     KEDEVVVYGKLITTKRKAAWYGDHPFVYRYSGRMKKALPWTEELKELKEKVEDHSKEK
                     FNSCLLNLYHTGEEGMSWHSDAEKELKKDGAIASVSFGAERKFCFKHKELKDLKASIL
                     LEHGSLLVMKDKTQTYWLHSIPVSKKIITPRINLTFRTIVF"
     gene            6858..7298
                     /locus_tag="PLF66_24240"
     CDS             6858..7298
                     /locus_tag="PLF66_24240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67221.1"
                     /translation="MKKYLFLVLLLFFSHCTTVIQGEFQSKGKSVIDFQFTPTRCYSG
                     ERLQFHGVILLDQNPNGIRITGIEDQFLGKIIKIRIPSLCQTEDDCEEIFLSKKVCSK
                     YKFQIQPTNVRINSIRAIKGNLELECNLPNGSILSGKSNFEYCY"
     gene            complement(7360..7563)
                     /locus_tag="PLF66_24245"
     CDS             complement(7360..7563)
                     /locus_tag="PLF66_24245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67222.1"
                     /translation="MFLRALRGENLAFIKSSDKQKSLSETLRLAISLSLGTATQTHLF
                     IDLLEKGKIKISFAIRIQCLSFV"
     gene            7652..8584
                     /locus_tag="PLF66_24250"
     CDS             7652..8584
                     /locus_tag="PLF66_24250"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HMZ67223.1"
                     /translation="MGEIKNIAVIGAGAVGCFYGGKLQKALYQVQFQSNYLFKAKTKK
                     LLVKSVWGNFDLPLEIFPDTENMKIADLILITAKSCDPKNDCLHYSKLIRNILGPKTI
                     ILVLQNGINMEERLQKVFPKNPILGGLAFTCINRISANEIHHIDYGQIKIGPVHKKYE
                     EEAKLITQIFNDSGIDTLLEKNLRKARYEKLLWNVPFNSLSVLGNKASTKEIIDFEPL
                     CSLSKNLMLEVISIAKSEKIVLKKSLADEMIRRTKIMKPYKTSMLLDYEKGNKMEVES
                     ILGEVYQNGKKNKINTPNIEYTYNLMQFLNRNQK"
BASE COUNT         2941 a         1495 c         1360 g         2846 t
ORIGIN      
        1 cgtaaaggct taagtatatt atctaacgtc tcacttgtct gtttattaaa ggaagaagaa
       61 ttatttaata gaatttgatt tgcgatttta ttgaaatcat cctgaaactt tagattaatt
      121 ctttcaattt cttctttttg tttctgggaa aattcctcca tcgattttag ctcaatcatt
      181 ttagcggcta ggtttttttc caactcaatg gatttagttt ggagagattt gtattctgca
      241 ttctgggctt ccattttgag tgataactca tttgattttt caagacttgc tcgaagctct
      301 gactctcgaa ccgctaaatt ttgcccaagg tatttatttt cttgttcttt tgtcttcagt
      361 aactcttcct tttcagaaag agttttactg aattggtcta attttgtctg taaaatggca
      421 agattgtttt gtaaacttag aatctcctct ttggatagac cggtctccga ctgatatttg
      481 aattttgcta agaaaaatcc acccaatccg aatataatca tcacaatgag atttgtaatt
      541 aatacaattt ccaatggtag ctccttttta tgtcatattg tgtattgaat cgttcaaaaa
      601 tactcatttt tattttatta gagggtctgg acaaaatgga atttttttaa aaaaattttg
      661 caacctttcg aaacaatttg catcgtattt atcagacaat gaggtagctc atttatttta
      721 aaaaaggtaa atttatggaa acacaaattc aaactaaaag aacattggga tcatctatct
      781 ctctaatagc atttatccta attgcctcta cagtattatt cgtattcaca gttcctttta
      841 catgggaatt attctgggta gcattttttt cttattttat ccgaatgttc gcaattacag
      901 gagtttatca tcgctatttt tcgcacaacg cttacaaaac ttctagagta atgcagttcc
      961 tactggcatg gcttgggtgt atggctcaac aaaaaggacc gctctggtgg gcagcacacc
     1021 atagaaacca ccataagtat tccgacacag agaaagattt acattctcca agactcactg
     1081 ggttctggaa ttctcacatg ctgtggttct taaaaaacga tttcaatgac tatgatccta
     1141 aaatcatcaa agattacaca aagtatcctg aaattgtatg gctcgataaa tatcactgga
     1201 ttccaccttt attattttca atcgttcttg gaatgattag tttccctgtt ctggtttatg
     1261 gttatgcagt tgctactttc ctactaggtc atggaacttg gacaatcaac tctctctccc
     1321 atgtaattgg gaaacctcgt tttgatgctg gtgatgatag caagaataat tggttccttg
     1381 caatcattac aatgggcgaa ggctggcata ataatcacca ctattacaga cattctgcaa
     1441 accaaggctt ctattggtat gaatatgact ttacctacta tatcttaaag ctcatgagtt
     1501 ttgttggact tgtttgggat ttgaaaaaac cacctgttca tgttctggaa gaaggtatga
     1561 agttagacgc tatgagaaag ggtaaaaaag aatttaaaaa cctttctggt caatgggttc
     1621 ctgtcaccgt aagaattccg acagctcaaa cacaagcata attcccttat ccatttttat
     1681 aaattgccct ctactcgagg gcaatatctt cttttttaaa gttttttaaa aaaatctgtt
     1741 ttttttattc aaaataccgc tcatatttcc attttttttc tacatataga atagattcac
     1801 ttcattcgtt tccaattcta attcaatgaa atgactccct ttatacatca actaacgatt
     1861 taccaatcta agatagaaac gaggccaact taaaatgaaa aactctatat ctaaaaccaa
     1921 atttttttcc caaacaaata atctcgattt agctgagttt agctcaactg ttctaattcc
     1981 cattcaatac tttgatctct tctcaagaaa aattcaaaaa cacaaaggaa ttcgcagcta
     2041 tattcattat ctcttgatta aatataaact tcatatagcg aacggacttg tgcctagcta
     2101 ttcgaaggta actacaaaat accaggaaaa aggtcaagaa ctgcaaaaag ttgcttttcg
     2161 tccaaaacct gaagactggg cggagttaaa actttatcga atcagctttg gtatgtcgat
     2221 ctctgccttt ttagtctatc tactaatagc ggactctgta gactttgcag agaccctatc
     2281 cgactaccta accggtgtcg gaataacaac gaccccaaat tttaacttga ccgcaaaagt
     2341 ctatctttgg aacgaaagaa cttactacgc tactattttt caataccgga atattcccta
     2401 ccattgaaag tagaaatccg ttataataaa atcctaaaac tatttcgtta gaggttgaat
     2461 tacatccttt gaccattggc taggttcatg accgtaaatt acttttactt gtggaaattc
     2521 tttcacaaaa tttcgcattc tagatagtgc gttagacgct ttagttttat caaccgtgaa
     2581 accaggaacc acattatttt cgaaaccggc ttttaaatga gaggaatcga atgtaaatag
     2641 gaaagagcct ttcgaagaaa caacgagagc agcaagttct ccgtttgtat gaccacttgc
     2701 atcaatgagc cagatagatc catcaccaaa caagtcgtag actctaccaa gtttgggcat
     2761 attaacataa tccgcattgg caagtgtgag aaggttaaaa tttaaagata aaggttccat
     2821 tggatatcca tggagaatac gaaatgcttc actcgctgat ttcttttcat cttctgaaat
     2881 caagacattc aaattacctc gtttcaatag atcccgaact ccacccaaat gatctccatg
     2941 cagatgagaa ataatagcaa aattcaaatc agagttctga atagcaagtt tactcaaaag
     3001 actagcaata tccgtcgccg gctctacctt gcaagcggtt ttatagataa agccatataa
     3061 ggaaaaatca cacctaccgt ctttatcggc tgtcggaact ccggtgtcga ataaaaagta
     3121 accttctgac ggatgcgata ctagataact cattgcaggc acccactgat ctatcttttc
     3181 tttttctggt gtattggaat tttccttatc aatgagaacg gatggacctg tataaacatc
     3241 gccattctta agtgcagtta tctgaattgg ctttgcggat ttaaaaacat cattccaatc
     3301 tttaaaaacc tttttaggat tcattttagg ttgccagtgc tttgattctt ttttgagagg
     3361 tgcagctaaa aaacagttca aaaagactcc gagggataaa attaaaaaaa actttaaata
     3421 cattgctttt tccttgaaaa ataaattaag taaatactag cataataact cttatcagtc
     3481 tggaacttta gttccttttt ttcaaaaata tggaagatta taaaaatata ttacagaata
     3541 atattttctc ctctcttaac aagaatatac ttaaaaagat tttccaggaa ggtgaaattg
     3601 taacatataa aacaggtaag attatttata gcgcagagac atctgataaa ataggactca
     3661 ttttaaaagg attgtttaga gttttcatct actccgccaa tggcaggcaa ttgacaatac
     3721 gatatgcgca agaaggagag ctattaggaa ttgttagttc catcaagaca ggaactgatg
     3781 tgggggtcca atctttggaa acatcaacaa tactaatatt ttccatcaaa accttgaaat
     3841 ctatttctat acaatatccc gaattagcat ggaaaatagc tgaagaatgt tccacccgag
     3901 tttatgattt aataaaagaa tttcatagac ttgcatttag aaacgttaga caaagattag
     3961 cttttcattt actatctata tcaaaaagta atagtattaa accctattta tcaattggaa
     4021 tttcgcaaca agacttagca gatgcttcag gatctgttcg tgaagtaatt gtaagagaat
     4081 taagacattt taaaaaacaa ggattcattc aatcaaaggg aaaacaaatt gaaattttaa
     4141 acccgaaagc tttgcttgaa gaatcgaaag tggatattga ataatatttg gaggattaaa
     4201 atggaatttg aaaaatggaa taaaagaaat attgcattcg cgtctggatt tagcttggtc
     4261 gggatggcaa ttaccgcagg attttctttt ggatatgcaa tcaatcaaat atttgtaagc
     4321 ggcaacgcag aattaacctt taataatatt atccagcata acttattatt tcgtctagga
     4381 atcttaggat tgttgattac tgctctacta gatatacttg tttccggttt attttattta
     4441 tattttaaag aaacaaatag aaatatatcc ttgttaaccg ctctttttcg aatctgctat
     4501 tcttctgtct ttatcttcgc tattcttcaa ttctgtttcg tattaatcct aaccaaagac
     4561 tccgattatt taagctccct tcctaaagaa aaaatcattt tatttataat ggaaggattt
     4621 aatcaaattt tcgctatctg tttaatgatc tttgcatttc atttacttgg attgggttat
     4681 ctagtcttaa tttcaaaaga aattcctaat attttgggaa tacttctaat ccttgcatca
     4741 ttctgttatt taggcacaag cacaggtagt ttacttatcg cagattacgg aagatataaa
     4801 tctacaattg atagtatagt tgcattgccg agtgcgatag gagaattagc catagccttt
     4861 tggctaatat ggaaaggtgg aaaagtctga ctcgtttccg catcagtagt attttttcta
     4921 agagctctat tatttattac caaagaaaac aatgagcagc gataagggat tcgataattc
     4981 aaaattattt attgcttctt aatcgttggt ttatctgcag caattcttta ctaattagaa
     5041 ctctcgttaa aataaatatt ttttatttac tttctacttt ctcatataag gctagttctt
     5101 aaactggtat ttgttcggct aacttcgagt aaccacagag gatttatgga aacaattgaa
     5161 atcaatgacg aattttcttt tccgccagag aatgtatggt cacttcttac agatcatgga
     5221 aagtatgcaa cctatgcccc actcaaagaa tccagtctag tgaaagaagg gaaaattgac
     5281 cgaaatggat tgggtgctca aagaaaactt gttactgata aaataatctt tctagaagat
     5341 atcacacact ttgaaccgtt tttacgattt gattacttaa ttgtagactg ttccgttcca
     5401 atgaaacact atggaggaat tattcgcttc gaaaaagttg agagaggaac aaagatccat
     5461 tggacatcta gctacgacat gttaggtgat acaagcccag cactccttcg tgctattaaa
     5521 tcatctagca ctaaaatgtt taaagaaatt ctttctggag taaaacggga attggtaaaa
     5581 aactgaatca catttattta ctctatatga accttgactc tattaataaa aacatatact
     5641 acctaaattt aaaagaatta aaactactat gcgatattca taagattccc tataaaattt
     5701 tatacaaatc aagagatggg aagattaaga acacaggaat attagatcgc aaagaaaaaa
     5761 tcattcagag aattctttta tttttaaaag aaagaaaaat aggaaaacct actctcttcc
     5821 cacaaaatgt aattctccaa acagaaatta caaatctcac ccctaaagaa aaaatctatt
     5881 ttggtctcta taaaaatgga aacaaagaaa ttctagaact attaaaaagt ctcactgaat
     5941 ctaaattcaa atttggagca actgctcaat tgattctact ggattcatgg gcaaagggaa
     6001 aactactaac gtttcaagag ttcagtcaaa tttggttaaa agaatctcag aagaaaattt
     6061 ctcatcccga atgggcatat ctttccgacc ttgcgaatgg taatgtaaaa gattggaaaa
     6121 acaaaagaag tgagattgca aaagaagtta ttgaaagcat caaaaaacaa tcgttctaaa
     6181 tgttaaatta attcttggtg taataatttt tttacttact ggaattgagt gaagccagta
     6241 agtctgcgtt ttatctttca tcactaaaag acttccatgc tctaataaaa tagatgcttt
     6301 taaatctttt aattccttat gtttaaaaca aaattttctt tccgccccaa agctcactga
     6361 tgcaattgcc ccatcttttt ttaattcttt ttctgcatca ctatgccaag acattccttc
     6421 ctctcctgta tgatataaat ttaataaaca ggaattgaat ttttctttcg aatgatcttc
     6481 tactttttct tttagctctt ttaattcctc tgtccaaggc aaagcttttt tcatcctacc
     6541 agaatatcga tagacaaaag gatgatctcc ataccaagca gcctttcttt ttgttgtgat
     6601 taattttcca taaaccacaa cctcatcttc cttccaatgg attgtctgta aaagttgagt
     6661 caaatattga tttgcctctg aatcagaaaa tatctttccg taatagttta caattccatc
     6721 ttttggaaga atgttttcta gattatctgc tgggaataaa ttcatggttt tccactatat
     6781 aaaaacagat ttagattcat tcaatagaaa aagtttgacc taaatagctg aaatagtttt
     6841 ttatattgta aataattatg aaaaaatatt tattcttagt cttattatta ttcttttccc
     6901 actgcacaac tgtaattcaa ggcgagtttc agtccaaagg aaaatctgtt atcgattttc
     6961 aatttacgcc aactcgatgt tactcgggag agagattgca atttcatgga gtgatccttt
     7021 tagatcaaaa tccaaatggg attcgaatta ctggaatcga agatcaattt cttgggaaaa
     7081 ttattaaaat aagaattcca agcctttgtc aaacagagga tgactgcgaa gaaatatttc
     7141 taagtaaaaa agtctgctca aagtataaat ttcagattca acctactaac gttcggatca
     7201 atagcatcag agcaattaaa ggaaatttag aactagaatg caatctcccg aatggatcaa
     7261 ttttatcagg aaaatccaac tttgaatatt gctattgact gaaagtccct tctctttaat
     7321 gctagatttg aattcattct ttatactagg taaatgacat tacacaaaag ataggcattg
     7381 gatacgtatg gcaaaagaaa tttttatttt tcctttttct aatagatcaa taaataagtg
     7441 ggtttgggta gccgtcccaa gcgatagact gatcgctagt cgaagtgttt ccgaaagact
     7501 cttctgttta tcactggact ttataaaagc aagattttca ccacgaagtg cacgaagaaa
     7561 cattgtaaat ttccgaagaa attttgacct ccgtgctctt catggttaga ttttttaaaa
     7621 gcttttacac agtcttggga agtaggtaaa tatgggtgaa attaaaaata tagctgtcat
     7681 tggagcggga gctgtcggat gcttctatgg aggcaaatta caaaaggctc tctaccaagt
     7741 tcaattccaa tcgaattact tattcaaagc aaaaacaaaa aaactattag taaaaagtgt
     7801 ttggggtaat tttgatttgc ctctagaaat atttcctgat acagaaaata tgaagattgc
     7861 agatctcatt ttaatcactg caaaatcttg cgatcctaaa aacgactgcc ttcactactc
     7921 taaattaatt cgaaatattt taggaccaaa aacaattatt ctagtcctac aaaacggaat
     7981 caacatggaa gaaagacttc aaaaagtttt tcccaaaaat ccaattctag gtggactcgc
     8041 ttttacttgt atcaatcgaa tttcagcaaa cgaaattcac catatagatt atggacaaat
     8101 caagattgga ccggttcata aaaaatatga agaagaagca aaactcatca cccaaatatt
     8161 caacgattct ggcattgata cactccttga aaaaaattta cgaaaagcac gatatgaaaa
     8221 actcttatgg aatgttccat ttaattctct ttctgtttta ggaaataagg catctacaaa
     8281 agaaataatc gattttgaac cactctgctc tctttcaaaa aatctaatgc tagaagtgat
     8341 ttcgattgca aaatcagaaa agattgtgct caagaagtct ctcgccgatg aaatgattcg
     8401 aagaacaaaa ataatgaaac cttataaaac atcgatgcta ttagattatg aaaagggaaa
     8461 taaaatggaa gtagaaagta ttctaggaga agtatatcaa aatgggaaaa aaaataaaat
     8521 aaatactcca aacattgaat acacatacaa tttaatgcaa tttttaaatc ggaatcaaaa
     8581 atgaattcta tcttaatcaa aacaatattg ggttttttaa atttattcgt aactatgtct
     8641 tt
//
LOCUS       DAOQOS010000185         8473 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_4584_length_8473_cov_5.593728, whole genome shotgun sequence.
ACCESSION   DAOQOS010000185 DAOQOS010000000
VERSION     DAOQOS010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 8473)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8473)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8473
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4584_length_8473_cov_5.593728"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..405)
                     /locus_tag="PLF66_24255"
     CDS             complement(<1..405)
                     /locus_tag="PLF66_24255"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar nucleotide-binding protein"
                     /protein_id="HMZ67224.1"
                     /translation="MELTNKHFLITGGKGMLATDLQKALEKENCKITAIDVDELDILV
                     EKDVHGKIAALKPDILINCAAYTAVDKCETESIAYEINGTALKYLSEACANNKIKLVH
                     FSTDYIFSGNFKTPIPEDQAPAPINQYGHGKLR"
     gene            complement(396..827)
                     /locus_tag="PLF66_24260"
     CDS             complement(396..827)
                     /locus_tag="PLF66_24260"
                     /inference="COORDINATES: protein motif:HMM:NF017880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF952 domain-containing protein"
                     /protein_id="HMZ67225.1"
                     /translation="MSETEELPEYIYHLVLKNEFIPLVRDNYYLPLRFQQDKFIHCTQ
                     GEDLTIIVANDYFSGITDLLVLKIRLKAIHSPVTFEKPIPIPGGKKNHLIEGVLFPHI
                     YGPLNLDAIEGIGILPKINYVFHFPKKFISYTQLKEGKLWN"
     gene            complement(829..1938)
                     /locus_tag="PLF66_24265"
     CDS             complement(829..1938)
                     /locus_tag="PLF66_24265"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004777886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HMZ67226.1"
                     /translation="MSSLQNYIQKELEEIKNKNRYRFLKIPNGIDLSSNDYLCLSKNP
                     KLISAFKEGIDLYGTGSTASRLIRGHRDIHEVVEEKFANWVKSETSLFLANGFSANVG
                     LMEAISNPRTFVFTDRLNHASILDGIRLSQANKKYYHHLDMNHLEEQLKKTNSKDQKI
                     IVSETVFSMDGDLAPISELIYLKKKYDACLILDEAHALGVFGEEGSGLSNAFQNKELN
                     EIDFRVYTGGKSLGLEGAFIATSNLYKEYLINTMRTFIFSTAPSPAIPYALLTSIDLV
                     KGMDEERIAILKNAEELRNDLKIKNYETLSSKSQIVPIVLYEETKALQFAQKLQEKGF
                     DIRAIRPPTVKESRLRVSINAGITPNIIKYLLNSL"
     gene            2382..3125
                     /locus_tag="PLF66_24270"
     CDS             2382..3125
                     /locus_tag="PLF66_24270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67227.1"
                     /translation="MFEGILKKSVKILIVIILLNCMTADPDRTQYQVQKENFDWGRYA
                     VKKFEDKVFRVKIFSGDYPDDLFEELGTVFVKDGDKSAVYYLKSYYDIFLLEIENKTD
                     LPQDFSIPRIQFIGQECKDQFATPWSLPNDVYRLNPKGITKNVYNGIVVLGLAVALAA
                     ISYSIPNILMGASSSKDKKETELTKVEPTEKEKKFYWSSLFHETKYQYNDLISQTNRI
                     EPNSKKIGLLFLAKDSFDKCRDSLILQVE"
     gene            complement(3177..5126)
                     /locus_tag="PLF66_24275"
     CDS             complement(3177..5126)
                     /locus_tag="PLF66_24275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67228.1"
                     /translation="MKVYVFILFLLIGISISAKENKIYVKGIETNLSEATVKKIENIL
                     LLSIIRNYPEANVFSDDASLSVLKQGKLSQSIGADSEFLQYLGNSLNFQELITSNLQK
                     NASNFQFSIQSIKLGTKENDFSIQNKVYFEFKESEINFIIPEMVKALKDKKYSILKTP
                     ISNSNIDLTEIILPQANNVLLEPKQFESKDSLPITFLEGVSNYIEKADTEYKNKNYLE
                     ALNIYLKILETIKTLENSTEKNIKNITQNIQKRANLSAQAAYSELIKTKDQTLPKDFS
                     NTNGDLIFSFFQGYIEDWKKYKDLPNFAKTDLIERNFKSRTMGLMKIYLSRLEKAGDE
                     KYNLLQFKEAIEEYKKIESVYNPYSDLLDLRNYQKLIQSKIKTTKESATSYTSSKVRG
                     YLQFAESENARAVLEKNMQNTEALLDRKNSSLYFLKEARSLIVEYREFVDEQTFKHFE
                     SISTSFNLDNERHTFITPQNIALAPVRYIGNIFRGISDIFIFKWGYGIGIGSEFFVFG
                     LSPILYNEYFVETSTAYGIQTEYSKGSELSSIDSAFQDKEKNIPNMGKSIGFYGVYTG
                     NCPSYFFRMCRTQLDKENTDYRKYTTLNVSIGFGPSVYFGLELHRIPEFFGVLIFQDW
                     DLLNQKKIAKPRYRYFGVENIKPIE"
     gene            complement(5501..6502)
                     /locus_tag="PLF66_24280"
     CDS             complement(5501..6502)
                     /locus_tag="PLF66_24280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67229.1"
                     /translation="MKNILFLSLLFQSAVFSEKYGPYDLAFPSAVSAGTASLGVGQEN
                     SSSNLYTNPSLLSNQNKSILDGGLSVSTNQNKYNALRPTSLGFYVPINATSGVGLTGK
                     QSFYQNFPSGYDSMSNYTYSFFGSFKLNDNWNASIGIGPSVVYRGGYQSSYSISPQAS
                     LSYKKEKHLVGAFFQSTGKFRLEGYRSADSLRERIPEFFAIGYSYDLGQTKIYTEIRK
                     IFWEYSSFKLNGNNSKPPLDRGIGAEFKYSLGFNHSISDTNFQIRSGIELGGFYNEKG
                     QNKRSVGLAIGGSWKVYQSKEEDSFLIHFAIVNYSIFSKSGGRQPETQFYFSGSYLY"
     gene            6569..7582
                     /gene="argC"
                     /locus_tag="PLF66_24285"
     CDS             6569..7582
                     /gene="argC"
                     /locus_tag="PLF66_24285"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012476246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HMZ67230.1"
                     /translation="MKVSIIGAGGLTGRELIGLLANHPDIEIVHVTSNKTAGKKISEI
                     FPNLRFKKDLVFKTHEEEIPKESLVVLAVPNEVSLAMTPNLLEKGHKVIDLSGAYRLH
                     NKDLFEKFYNLKHDSFHLMEKVVFGIPEMFRDKLKGADFVSNPGCYATSGLLPLFMLG
                     SFREKISGPIVIDSKSGVSGAGGRTEDIGFTFTNTYENFRAYKILSHQHTPEIEEYAG
                     YKLSSKLPKIVFTPHLLPVYRGILSTFVISFQEAIDPSQLANQFSKFQSEPFIRLYKK
                     PEEVELKNIQNSNFLDFSYKLEENILVIVSALDNLVKGAAGQALQNINLMLGLEETKG
                     LLA"
     gene            7667..7739
                     /locus_tag="PLF66_24290"
     tRNA            7667..7739
                     /locus_tag="PLF66_24290"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7700..7702,aa:Lys,seq:ttt)
     gene            7755..7826
                     /locus_tag="PLF66_24295"
     tRNA            7755..7826
                     /locus_tag="PLF66_24295"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7788..7790,aa:Glu,seq:ttc)
     gene            8052..8465
                     /locus_tag="PLF66_24300"
     CDS             8052..8465
                     /locus_tag="PLF66_24300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67231.1"
                     /translation="MYRVDRISAKDISIDYNITGNVKIGLTILAVDKTYEFNQIISHL
                     RCNVFSSCIDYTKMNPWTGKLYTMYFHFLSPSIYLEPLEDCLIFLEKQFLIILLFGSL
                     TVILLLNISLYPIQFLRLITTISLLRNVKELKVSL"
BASE COUNT         2701 a         1342 c         1396 g         3034 t
ORIGIN      
        1 tcttaatttc ccatgaccat attgattgat tggggcaggg gcttggtctt ctggaattgg
       61 agtcttaaaa tttccagaga aaatataatc agttgaaaaa tgaactaatt tgattttatt
      121 atttgcgcaa gcttctgata aatatttgag agctgttcca ttgatctcat atgcaataga
      181 ttcagtttca catttatcaa cagcggtata agccgcacaa ttgatgagta tatctggttt
      241 taaagcagca attttaccat ggacatcttt ttctacaagt atatctaatt catcaacatc
      301 gattgctgtt attttgcaat tttctttttc gagagctttt tgaagatctg tggctaacat
      361 acctttgccc ccggtgatta gaaagtgttt atttgttaat tccataattt gccttcttta
      421 agttgagtat aagaaataaa tttcttcgga aaatgaaata cataattgat tttaggaaga
      481 atcccaatac cttcgattgc atctagattg agaggaccat aaatatgagg aaataggaca
      541 ccctctatta gatgattttt tttcccacct ggaattggaa ttggtttttc aaaagtaact
      601 ggagaatgaa tcgcctttaa acgaatcttt aaaactagta aatctgttat accggaaaaa
      661 taatcatttg ctacaataat ggttaaatcc tcaccttggg tgcaatgaat gaatttatcc
      721 tgctgaaaac gaagtggcaa atagtaatta tccctaacaa gcggaataaa ctcattttta
      781 agaactagat gatagatata ttcaggtaat tcttctgttt cactcatttt ataaactatt
      841 caaaagatat ttgataatat ttggtgttat tccagcattt atcgagacac gtaaacggga
      901 ctctttcaca gtaggaggtc gaatggctct aatgtcaaat cctttctctt gtaatttttg
      961 cgcaaattgc aaggctttcg tttcctcgta gagaacaatt ggaacgattt gagattttga
     1021 ggaaagtgtt tcatagtttt ttatttttag atcattccgt aactcttctg cattttttaa
     1081 aattgctatt cgctcttcat ccattccttt aaccaagtca atagacgtta gtagtgcata
     1141 aggtattgca ggacttggag ccgttgaaaa aataaaggtt cgcatagtat tgattaaata
     1201 ctctttgtat agattagatg ttgcaataaa cgctccttct agaccaagag acttcccacc
     1261 agtgtaaact ctaaaatcaa tttcatttaa ttctttgttt tgaaaagcat tggaaagacc
     1321 tgaaccttct tctccaaaaa ctcccagagc atgcgcttca tctaaaatga gacatgcatc
     1381 gtattttttc tttagataga tcaattcaga gataggtgca aggtctccat ccatactaaa
     1441 gacagtttcc gatacaatga ttttttgatc ctttgaattt gtttttttta attgttcttc
     1501 tagatgattc atatctaggt gatgatagta ttttttattt gcttgcgaca gtcgaattcc
     1561 atctagaata gaagcatgat ttaatctatc agtaaaaaca aaagttcttg gatttgagat
     1621 tgcttccatg agacctacgt tagccgagaa accattcgca agaaatagag aagtttctga
     1681 ttttacccaa tttgcaaatt tttcctctac gacttcgtga atatctctat gacctcggat
     1741 gagcctagag gctgtggatc ctgttccata gagatcgatt ccttctttaa atgcggaaat
     1801 taacttggga tttttagata ggcaaagata atcatttgaa gataaatcga ttccattcgg
     1861 aatctttaaa aatctatatc gatttttatt tttgatttct tctaattctt tttgaatata
     1921 attctgaagg gacgacatct caatactata attccaagat taatctttct atttaatttc
     1981 caagtcgaaa agagttctga tgtttataat tcttttttat tcttcaatag gttttaagat
     2041 gagaattgat atgtagtaat ctgaatcaat tgaaaataga tttaaagttt gttgactctt
     2101 aaatattatt aaattttata ataatagatc cctcaccttt cgcacaaaag tataagagag
     2161 agaagggaaa attttagcta ccttagttct tagcactcag tggatcgttt gagttcttgt
     2221 ccacctactt aggtattgat tcattggaaa aatctaatag aatcatatct attattttta
     2281 tttatctaat tctatacttt gtgaaatgta agttaataaa attttaattg gccgtttggt
     2341 agacagctaa attggttttc cagtttatta agggagcttt tatgtttgaa ggaattttaa
     2401 aaaaatcagt aaaaatctta atcgttataa ttcttctgaa ttgtatgact gcagatccag
     2461 atagaactca atatcaagtt caaaaagaaa attttgattg gggtagatac gctgttaaaa
     2521 aatttgaaga taaagtattt agagtaaaga ttttttcagg agattatcca gatgatttat
     2581 tcgaagaact tggaacagta tttgtaaaag atggagacaa gtccgcggtt tattacctaa
     2641 aaagttatta tgacattttt ttgttagaga ttgagaataa aactgatttg ccgcaagatt
     2701 tttctattcc taggattcaa tttatcgggc aagaatgcaa agatcaattt gcaactccct
     2761 ggtctcttcc aaacgatgta tatcgtctca atccaaaagg aattacaaag aatgtttata
     2821 atggtattgt ggttcttggg ttagctgttg cgttagctgc tatatcttat tcaattccaa
     2881 atattctaat gggagcttcc tcttcaaagg ataaaaaaga aactgaacta actaaggtag
     2941 agcccacaga aaaagagaag aaattctatt ggtcttctct ctttcatgaa acaaaatatc
     3001 aatacaatga tttaatcagt cagacaaata gaatcgaacc gaatagtaaa aaaataggac
     3061 ttcttttttt agctaaagat agttttgata aatgtagaga ctctctcatt ttgcaagttg
     3121 agtaaataaa ccactaggtc atttataaaa aaatgaactg cattttactc gcggttttat
     3181 tcaatcggtt ttatattttc tacaccaaag tatctatatc taggttttgc tattttcttt
     3241 tgattcaata aatcccaatc ctgaaaaatt aggacaccga agaactctgg gatacgatgt
     3301 aactctagac cgaaataaac acttggtcca aatcctatag agacatttag agttgtgtat
     3361 tttctataat ccgtgttctc tttatccagc tgtgtcctgc acattcggaa aaaataagaa
     3421 gggcaatttc ctgtatagac accgtaaaat cctatacttt ttcccatatt cggaatgttt
     3481 ttctccttat cctgaaaagc actatctatg ctactcaatt cagatccttt actatattct
     3541 gtttggattc cgtaggctgt ggatgtttca acaaaatatt cattgtagag gattggactt
     3601 aatccgaaaa cgaaaaactc agatcctatt ccaattccgt atccccattt aaaaataaaa
     3661 atatctgaaa tcccacgaaa tatatttcca atgtatcgaa ctggggcgag cgctatgttt
     3721 tgaggagtaa taaatgtatg acgttcgttg tctaagttga aagatgtaga aatagattca
     3781 aaatgcttga atgtttgttc atctacaaac tctctatatt cgacaatgag gcttctcgct
     3841 tcctttaaaa aataaagact agaatttttt ctatctaata gagcttctgt gttttgcata
     3901 tttttttcta atactgctct agcattttct gattctgcaa attgtaggta tcctctcact
     3961 ttgcttgatg tataagaggt ggctgattcc tttgttgtct ttattttgga ttgaattagt
     4021 ttctgatagt ttcttaaatc caatagatcc gaatacggat tataaacaga ctctattttt
     4081 ttatattctt ctattgcttc tttgaattga agtaaattgt atttttcatc tccagctttt
     4141 tctaagcgtg aaaggtaaat cttcattagc cccatcgtcc tcgatttaaa atttctttct
     4201 ataaggtctg ttttggcaaa atttggtaag tccttatatt ttttccagtc ctctatatat
     4261 ccttggaaaa aggaaaatat tagatctccg tttgtatttg aaaagtcttt tggaagagtt
     4321 tggtctttag tttttattag ttctgaatag gcggcttgcg cagatagatt tgctcttttt
     4381 tgaatatttt gtgttatatt ctttatattt ttttctgttg aattttctag agttttgatt
     4441 gtttctaata tttttaaata gatattgaga gcttctaaat agtttttatt tttatattct
     4501 gtgtctgctt tttcaatata atttgaaaca ccttctagaa aagtgattgg aagactatcc
     4561 ttagattcaa attgcttggg ttcaagtaaa acgttattcg cttgtggaag aattatttct
     4621 gtaagatcta tatttgaatt agatataggg gtttttaaaa tggaatattt tttatctttt
     4681 aaggctttca ccatttcagg aattataaag tttatttccg attctttaaa ttcgaaatag
     4741 actttatttt gtatggaaaa atcattttct tttgttccta atttaatact ttgaattgaa
     4801 aattgaaagt tagatgcgtt tttttgtagg ttacttgtga ttagctcctg aaaatttaaa
     4861 ctgtttccta aatactgaag aaattctgaa tctgctccaa tggattgaga aagcttgcct
     4921 tgctttagaa cagagagact tgcatcatca gaaaaaacat ttgcctcggg atagtttcga
     4981 attatgctca ataatagaat gttttcaatt tttttgactg ttgcttctga caaatttgtc
     5041 tcaattcctt tcacgtagat tttattttct tttgctgaaa tggaaatccc tatcagtaaa
     5101 aatagaataa aaacatatac tttcattgga tacacctcaa agattcttat ggttaaacta
     5161 tagactgttc aagggacaaa gtagagattt tttttataga aaataaaatt agttcaaata
     5221 attttcagaa tggagtaata aataaaaaaa tctctccaat taccttttca tttttaaaga
     5281 gagtaatgct tttattttta aatgatttga ttcaggaaaa gaaggcgccc tttgcattgt
     5341 ttaaagattt aaatttcttt tttaaattat aaactagaag aggttaaaat gaaactcttc
     5401 ttttagagat aggaagactg taacaaaaaa tcatagaaaa gtggaaaaga tttatctact
     5461 tggattttag attagtcttc gagaatgaag gatcattttg ctaatacaaa taacttcccg
     5521 aaaaatagaa ttgggtttct ggttgtcttc caccactctt agaaaaaata gaataattta
     5581 caatcgcaaa atgaatcaaa aaagaatcct cttctttgga ttgataaacc ttccaactac
     5641 cgccgattgc taatcctacc gaccgtttgt tctgtccttt ttcattatag aatcctccca
     5701 attcaattcc tgatcgaatt tggaaattgg tatccgaaat actatgattg aatcctaaac
     5761 tatatttgaa ttctgcacct attcctcgat cgagtggcgg ttttgaatta ttcccgttta
     5821 atttgaaact actatactcc caaaaaattt ttctaatctc agtatagatc tttgtctgac
     5881 ctagatcata gctataacca atcgcaaaaa attcaggaat tcgttctctc aaagaatcag
     5941 ccgaccgata tccttctaat ctaaatttgc ctgtgctttg aaagaatgct cccactagat
     6001 gcttttcctt tttataggaa agggaagctt gtgggctaat ggaataactc gattggtatc
     6061 cacctctata aacaacacta ggtcctattc cgattgacgc attccagtta tcatttaact
     6121 taaaactacc aaaaaaggaa taggtataat tgctcattga atcatatcca ctaggaaaat
     6181 tttgataaaa gctttgctta cccgtaagac caacaccgct tgtcgcattg attggaacat
     6241 aaaagcccaa tgatgtaggg cgaagtgcat tgtatttatt ttggtttgtc gaaacactca
     6301 atcccccatc taaaatagat ttgttttgat tggaaagtaa gcttggattt gtgtatagat
     6361 tactactcga attctcctgc ccgactccta gtgatgcagt tcctgctgat acggcacttg
     6421 gaaatgctaa atcgtatgga ccatattttt cagaaaaaac cgctgattgg aagagtagag
     6481 agagaaataa aatgtttttc aaacgcattg tctatacaag tctggataat tagctttaaa
     6541 agcaatgagt tttatagaag gagttcaaat gaaagtttca attattggtg caggtggact
     6601 aactggaagg gagttgattg gccttcttgc aaatcatcct gatatagaga ttgtgcatgt
     6661 cacaagcaat aaaactgctg ggaaaaaaat ttcagaaatt tttccaaacc ttcgtttcaa
     6721 aaaagattta gtcttcaaaa cacatgaaga ggaaattcca aaagaaagcc ttgttgtgct
     6781 tgcagttccc aatgaagtct ctcttgctat gacacctaat ttattagaga aggggcataa
     6841 ggttattgat ttatcgggcg cctatcgttt gcataataag gatttatttg aaaaatttta
     6901 caatctaaaa catgattctt ttcatttgat ggagaaagtc gtgtttggta tcccggaaat
     6961 gtttagagat aaactaaagg gtgcagattt tgtatctaat cctggttgtt atgcgacttc
     7021 tggacttctc cctttattta tgcttggatc tttcagagag aaaatatctg gtccgattgt
     7081 aattgattca aaatcaggag tcagtggagc agggggaaga acagaggaca ttggatttac
     7141 ttttacaaat acctatgaaa attttagagc ttataaaatt ttatcacatc aacatactcc
     7201 tgaaatagaa gaatacgcag gttataaact gtcgtctaag ctacctaaga ttgtttttac
     7261 tcctcatttg cttcctgtat acagagggat tctttctact tttgtgattt cattccaaga
     7321 agcgattgat ccatctcaac tagcaaatca gttttctaag tttcaatcag agccttttat
     7381 tcgcctctat aaaaaaccag aagaagttga actaaaaaat atccagaatt ctaattttct
     7441 agattttagt tataaattag aagagaatat tttagtgatt gtatctgctc ttgataatct
     7501 agtaaaagga gctgctggac aagcacttca aaatatcaat ttgatgctcg gtttagagga
     7561 aactaaagga ttactcgcat aaattttttt tagaaaaaat ccttttttaa agttaaatgt
     7621 ttttgacatt ttgctaggaa taaaaaaact gacataagca acgcatgagt cgttagctca
     7681 gtcggtagag catttcactt ttaatgaagg ggtctcgggt tcgaatcccg aacgactcag
     7741 tagttgcttc ctaaggtgcc atcgtctagt ggttaggaca ccaggttttc atcctggtaa
     7801 ccggggttca attccccgtg gcactaaaat cctttttaaa aaattcactc aacgaaagaa
     7861 ttcccttata atcttactca tttagtaaaa atatttttta atgattttat tcaatgaact
     7921 gcctccctat agcaatgaaa gttcatttcc ttattctttt gtttcttttt tgggctcatc
     7981 aaatagattc cgaaacatag aacgataata ggcaaaagaa aaaagtatcg attcgtattg
     8041 gttataattt tttgtataga gtcgatagaa tttcagccaa ggatatctct attgattata
     8101 atatcactgg aaatgtaaag ataggcttaa caatcttggc agttgataaa acatatgaat
     8161 tcaatcaaat catatcacat cttcgttgta atgtcttttc ttcctgtatt gattatacaa
     8221 aaatgaatcc ttggacaggg aagctatata caatgtattt ccattttctg agtccttcta
     8281 tttatctgga gccattggaa gattgcctga tttttctaga aaaacaattt cttataatcc
     8341 tactctttgg atcattgacg gttattcttc tactcaatat tagtctttat cctattcagt
     8401 ttctacggtt aataactact atcagtctgt tgcgaaatgt taaagaatta aaagtcagcc
     8461 tttaaaaata aat
//
LOCUS       DAOQOS010000186         8471 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_4586_length_8471_cov_5.637476, whole genome shotgun sequence.
ACCESSION   DAOQOS010000186 DAOQOS010000000
VERSION     DAOQOS010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 8471)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8471)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8471
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4586_length_8471_cov_5.637476"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..934
                     /locus_tag="PLF66_24305"
     CDS             <1..934
                     /locus_tag="PLF66_24305"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HMZ67232.1"
                     /translation="LCLPLKNHGNTIGILYLENNLMPGAFTKSRIETVKLLSVQAVIS
                     IENARFYEELESKVKDRTKALNSTLDVIKKDLLIAQKIQQNTLPLDLERLGPLKFTIK
                     YLPMTEVGGDFFDIIKVRSGVYRIFRADATGHGVQAALVTMAIKSEYENLKHTISEPT
                     VMIDILNRTYQRRYSSLNSFFTCVVLDNNLHTDKLTYAFAGHPAQILIHNNEIIQLER
                     TGKMIGLSKTMNLTSKQYDFVLGDKILLFTDGIFEEFNQEGELFGEEKFNSIIYKNKM
                     ANNEQIISQVLKEVTKFKGGLEQDDDITMIGIEL"
     gene            1096..6144
                     /locus_tag="PLF66_24310"
     CDS             1096..6144
                     /locus_tag="PLF66_24310"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HMZ67233.1"
                     /translation="MEVPNIPENEKERHKKLLEYNILDTLEEKEFDDIAKLAAQICNS
                     PIALISLVDSDRQWFKSNIGLDAKETPRDISYCAHAILTDEILEIEDSANDPRFYDNP
                     LFLNTPIVRFYAGAPLITSDGYRIGTLCVIDRDAKKLNDQQLQTLKVLSNNVINLLEL
                     RKNLYETKDSEIILKDILDNTEDMIQNIQLSDGRILYANPSWLNTLGYTKEESKNLRF
                     NDIIHPESLDHCMFLYQKIANGEDIKDFHAIFITKNKSKVFVRGSSGCVFKNGKPYST
                     RGIFQNITRETESELNEFKLKSRLEYLLEHSPTIIYTSKVNDDFGATYISPNVEKILG
                     LNQNQFLKDSGFWLNSIHPEDRERIVIGLEQLFLNNKHVHVYRFLSGNGMYRLMRDEL
                     RLIRDSSEKPMEIVGSMVDITDSKEIELALQRSNVQVRDFAQALDAHGIVAITDSKGI
                     ITYANDEFCKISKYNRKELVGKTHKIVNSDFHSDYFFTVMWETIKNGNVWKGEIQNKA
                     KDGTLYWVDTTIVPFKNEKNQIYQYVAIRKDITKRKEAERSLIESESRFRAVTESANA
                     GIITVNMNRLIIGWNLAAQKLFGYYFNEAIGKNLAQLIPNINYKMFQDYIENYIYPNK
                     IENPQALEIKGERKNGILFPIEVSISTYEINTERFFTLFIQDISYRKNAEGALRRSEE
                     EYKSLVQNAPDIIMKVNLNDEITFINRVTPGLKVSEVIGVKTENFILPKYREIVRKKH
                     SESIFNRKNIQYETEFLNPDGTTSWYSTYVSPILLNEEVIGLTLVTRNIDERKKLEQR
                     LIDQNRFIQKITDYIPGMVGYISKDQKILYANTNYYQWYGVSPKQIEGMRLEELLGDS
                     FLKIEKYIERALEGEIQKFDSEIIKTNGEKSHLWYQYIPDIVDDTVVGFVVVSLDITE
                     QKLTERNLIRTKNKLNTILEAIPEGLIEVDLNGEIIFANKGAGDILNIEQLNRSEKYY
                     KTKLWKSEFLDAFGSYTNVEKLPLAAAIAIGNHLDPVEYKLVDENGEEKWISIQAVPL
                     IDSDHNVYGGVASFRDVTQRVKNEKVIHENEAYLKAIVSSIDDLIFLLDKKYRFLNCW
                     TNRPDLLAYPREFFIGKTIRETFGEDSDLSFLFEKSIEKVLQNKQNAYVEYEMDGEWY
                     SAKFNYVYLNQIQEEAVSCMAQSIHAKKQSELNLIRAKEEAERANKAKSEFLANMSHE
                     IRTPMNSILGFGELLYNSLQNEIQKDFAHSILTSGKILLKLINDILDLSKIESGKFNI
                     TLNPVNLRVVANDMRTIFYQKIIEKNIDLKIEIESNFPELILLDDIRIRQVLLNILGN
                     SIKFTENGFIRLVVKSRSSESVNNKVDISILIEDTGIGIEEEDQNRIFEAFTQSRKQD
                     QSKYGGTGLGLTIAQKLTNLMGGQIFLESEKGKGTKFWIDMENIEIYNHSSNILENVN
                     VLLGNENKQFEKSKILIVDDLPINRKLIYSYLKEFKELELFEATNGEEAIQVVNKEKP
                     DIILMDIRMPILNGYEAAKILKSKDETKDIPIIVLTASISENTKNEIMDICEGYIQKP
                     IQKKVLIGQLHTYLKSKFIQTKEVKLDESSIEEKVDIEKNKVLFKILKEEKVQQWQIH
                     SKILDMQEIQLFAEEIENLALEYYSPDIRKWASELKRSVKNFEIDTMNELLKSFLQII
                     ENLKSNIDKHDNKN"
     gene            6147..7229
                     /locus_tag="PLF66_24315"
     CDS             6147..7229
                     /locus_tag="PLF66_24315"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HMZ67234.1"
                     /translation="MKEKIILIVDDTQKNIQLLGQILTPVGYRIIVAMNGIQALNALE
                     IQKPDIILMDVNMPEMDGFEACKRIKENDLFKDIPIIFLTARTDSNDIMQGFEYGGAD
                     YITKPFNSKELLIRISYQLELKDQREELIRVNTENKQLLRVLLHDLRNPVGAIDVMCE
                     MLKQNSENKDKIINLISQATKNSMSILENVKTMFSVGDKYIPNLVLFNVKSTIESALR
                     IFDHQFNSKNISLEINIPDNLKAVVDGSSFTDSVFNNIITNAIKFSFPGSKVIVSAEE
                     IDDKVKLTVQDYGMGIPPKILENIFDISKPTSRIGTNGEKGTGYGMPLVKKFAELSKA
                     KIEIHSIEKPNEGHGTRVSLYLEKKI"
     gene            7280..8134
                     /gene="panC"
                     /locus_tag="PLF66_24320"
     CDS             7280..8134
                     /gene="panC"
                     /locus_tag="PLF66_24320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004475917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HMZ67235.1"
                     /translation="MEVIKNKIELNQRIRKLKLEGKKIAFAPTMGFLHEGHIELFNEC
                     KSNADISVTSIFVNPAQFNDPKDFEKYPRNTDSDLKKCEKALIDIVYLPEVNDIYPQG
                     KVPDINIRIPHLMRNLCATTRPGHFEGVLLVISNLFHIVEPDIAFFGKKDYQQYLIIK
                     EFAKVTGMNIDVIGVETIREKDGLAMSSRNARLSSEEREAATLIPRAFQLCKEYIYGK
                     GRDPKKIKSIFSEVILSSSKMRIDYIEVLNANNLGDLEVLTGEILIAVAIFCGEVRLI
                     DNIRLTLS"
     gene            8155..>8471
                     /locus_tag="PLF66_24325"
     CDS             8155..>8471
                     /locus_tag="PLF66_24325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HMZ67236.1"
                     /translation="MEIIIECILNPGVPCDSILNYTYVLWFVFGIVLIVLEFFIPGVF
                     IIFFGLGALVTSLFHYLFILSFGMQITTWIVAAFAIMLFGAKFLKDLFPSEESYEPTM
                     KESV"
BASE COUNT         3274 a         1049 c         1501 g         2647 t
ORIGIN      
        1 tttgtgttta cctctgaaaa atcatggaaa tacaattggg attttgtatt tagagaataa
       61 tttaatgcca ggagctttta caaaatcaag aattgagaca gttaaattat tatctgttca
      121 agccgttatc tctattgaga atgcaagatt ttatgaagaa ttggaaagca aggtcaagga
      181 caggacaaaa gctttgaatt ctaccttaga cgttataaaa aaagatttat taatcgccca
      241 aaaaattcaa caaaacacac tcccattaga cttagaaaga ttgggtccat tgaaatttac
      301 tattaaatat ttaccaatga ctgaagtagg tggagatttc ttcgatataa taaaagtaag
      361 atctggagta tatcgtatat ttagagcgga tgcaacggga catggagtgc aagctgcgtt
      421 agtaacaatg gcaataaagt ctgaatatga aaatttaaag catactataa gtgaacctac
      481 tgtgatgatt gatattttga acagaaccta tcagagaaga tatagttctt tgaattcttt
      541 tttcacctgt gttgttctgg ataataattt acatactgat aaattaacat atgcatttgc
      601 tgggcatcct gcacaaatct tgatacataa taatgaaata attcaattag agagaaccgg
      661 taagatgata ggtctttcta aaactatgaa tttgacatct aagcaatatg attttgtgct
      721 tggagataaa atacttttat ttacagacgg aatttttgag gagttcaatc aagagggtga
      781 gctctttggt gaagagaaat tcaattcgat tatttataaa aacaaaatgg ctaataatga
      841 acaaattata tcacaagttt taaaagaagt aaccaaattt aagggtggat tagaacaaga
      901 tgatgatatt acaatgattg gaatcgaatt ataagatttc taaatatttt actaatctta
      961 taaatagttt tgctatctgt agagtataag tagtggataa taaccgttat gcgaatgtat
     1021 tttaaataaa ctcacaatct acttgactaa atattgttct tcttttttta ctagacaggt
     1081 taggttaggc tgtatatgga agttccaaac ataccggaaa acgaaaaaga aaggcataag
     1141 aaattattag aatacaacat tttagatacc ctagaggaga aagagtttga tgatattgct
     1201 aaacttgcag ctcaaatatg caattctcca atagcactca ttagcctagt tgattcagat
     1261 cgtcaatggt ttaaatcaaa cattggatta gatgcaaagg aaacaccgag ggatatttct
     1321 tactgtgctc atgccatttt aactgatgaa attcttgaga tagaagattc agccaatgat
     1381 cctagatttt atgataatcc tctttttcta aatacgccta tcgttcgatt ctatgcggga
     1441 gctcctttga taacatctga tggatataga atagggacat tgtgtgtgat tgaccgagat
     1501 gcaaagaaat taaatgacca acaattgcaa acccttaaag ttcttagtaa taatgtaatt
     1561 aatctattag aattacgaaa aaatctatat gagacaaaag atagtgaaat tatattaaaa
     1621 gacattttag ataatacaga agatatgatt caaaatattc aactatctga tggtagaatt
     1681 ctatatgcaa atcctagttg gctaaataca ttaggatata caaaagaaga atctaaaaat
     1741 ttgcggttta atgatattat ccacccagag agtttagatc attgcatgtt cctttatcag
     1801 aaaattgcaa atggagaaga tataaaagat tttcatgcaa tttttataac aaagaataaa
     1861 tcaaaagttt ttgtgagagg atcaagtggg tgtgttttta aaaatggaaa accttattct
     1921 actcgaggaa tatttcaaaa tataacaaga gaaacagaat cggaattaaa tgaatttaaa
     1981 ttgaaatcca gattagaata tttattagaa cattcgccta ccattattta tacttctaaa
     2041 gtaaatgatg atttcggagc aacttatata agtccgaatg tagaaaaaat tttaggatta
     2101 aatcaaaatc agtttttaaa ggattccgga ttttggttaa atagcattca ccctgaggat
     2161 agggaaagaa ttgtaatcgg tttagaacaa ttatttctaa ataataagca cgtgcatgta
     2221 tatcggtttt tatctggaaa tgggatgtat cgtttgatgc gagatgaact aagactgata
     2281 agagattcat ctgaaaaacc aatggaaatt gtaggcagta tggtagacat cacagattcc
     2341 aaagagatag aactggcttt gcaacgttcg aatgttcagg taagagattt tgctcaagcg
     2401 ttagacgctc atgggatagt tgccattact gatagtaaag gaattattac ttatgcaaat
     2461 gatgaattct gtaaaatatc taaatacaat agaaaagaat tagtaggtaa aacacataaa
     2521 attgttaact cagattttca ttctgattat ttttttaccg ttatgtggga gactatcaaa
     2581 aatggaaatg tttggaaagg ggaaattcaa aataaagcaa aagatggaac cttatattgg
     2641 gttgatacaa ccatagttcc atttaagaat gaaaaaaatc aaatttatca atatgttgct
     2701 attcgtaaag atattacaaa aagaaaagaa gccgaaaggt cattaataga aagtgagtct
     2761 agatttagag ctgttaccga gtccgcaaat gctggaatta taactgtaaa tatgaatcga
     2821 ctcattatag gatggaattt agctgcacag aaactatttg gttattattt caatgaagct
     2881 atcggtaaaa atttagcaca actaattcca aatattaatt ataaaatgtt tcaggattac
     2941 attgaaaatt atatttatcc aaataaaatt gaaaatccgc aagctctaga gataaaggga
     3001 gaaagaaaga atggaattct atttcccatt gaagtatcga tttctacata cgagataaac
     3061 acagaaagat tttttacatt attcattcaa gatatatcat atagaaaaaa tgcagaaggt
     3121 gcattacgaa gaagtgaaga agaatataaa tcgttagttc aaaatgctcc agacataatt
     3181 atgaaagtaa acctaaatga cgaaattaca ttcataaaca gagtcactcc aggtctgaaa
     3241 gtatcagaag tcattggagt aaaaacagaa aattttattt taccaaagta tagagagatt
     3301 gttcgaaaaa aacacagtga atctatattc aatagaaaaa acatacaata tgaaactgaa
     3361 tttttaaacc cagatggaac gacttcttgg tattcaacct atgtcagccc aattttattg
     3421 aatgaagaag ttattggatt aacactcgtt acccgcaata tagatgaaag aaaaaaactg
     3481 gagcaacgtt taatagatca aaatcgattc atacaaaaaa taacagatta tatcccggga
     3541 atggtaggct atatatctaa ggatcagaag attctatatg caaatacaaa ttattatcaa
     3601 tggtatggtg ttagccctaa acaaatagaa ggaatgaggc ttgaagaatt attaggtgat
     3661 tcatttttaa aaattgaaaa atatattgaa cgtgctctcg aaggagaaat tcagaaattt
     3721 gattctgaga ttataaaaac caatggagaa aaaagtcatc tatggtatca atatatacca
     3781 gatattgtag atgatactgt cgtaggcttt gtagttgttt cccttgatat tactgagcaa
     3841 aaactcactg agcgcaattt gattagaaca aaaaataaac tgaatacaat actagaagcg
     3901 atcccagaag gtctcattga agttgactta aatggtgaaa ttatttttgc caataaagga
     3961 gcaggggata tattaaatat tgagcaattg aatagatccg aaaaatatta taaaacgaaa
     4021 ctttggaaat cagaatttct cgatgccttt ggaagttaca caaatgtaga aaaattaccg
     4081 ttagccgcag cgatcgcaat tggaaatcat ttggatccag tagagtataa attagtggat
     4141 gagaatgggg aagaaaagtg gatatcaatt caagctgtgc ctttgatcga ttcggatcat
     4201 aatgtttatg gaggggttgc aagctttaga gatgttactc aaagggtaaa aaatgaaaaa
     4261 gtgatccatg aaaatgaagc ctatttaaaa gcaatcgtat cgtctatcga tgatctaatc
     4321 tttttactag ataaaaaata tagattccta aattgttgga caaatcgacc agacttactt
     4381 gcttatccaa gagagttttt tatcggaaaa acaataagag aaacattcgg tgaggactct
     4441 gatctatcct ttttatttga gaaatcgatt gagaaagtat tacagaacaa acaaaatgct
     4501 tatgttgaat atgaaatgga tggagaatgg tatagcgcca aatttaatta tgtatattta
     4561 aatcaaattc aagaagaagc agtttcttgt atggcacaga gtatacatgc aaaaaaacaa
     4621 tctgagttga atttaattag ggcaaaggag gaagcagaga gagcgaataa agctaaaagt
     4681 gaatttcttg caaatatgag tcatgagatt agaactccta tgaattcaat tcttggattt
     4741 ggagaattat tatataatag tcttcaaaat gagattcaaa aagactttgc tcattcaatt
     4801 ttaacaagtg gaaagattct acttaaacta ataaatgata tattagattt atctaaaata
     4861 gaatcaggta aatttaatat aacacttaat cctgttaact tacgtgttgt tgctaacgat
     4921 atgcgaacta tcttttatca aaagattata gaaaagaata tagatttaaa aattgaaatc
     4981 gaatctaatt ttccagaatt aattctttta gacgatatac gaattaggca agtattactc
     5041 aatatattag gcaatagtat taaatttaca gagaatggtt ttataagatt ggtagttaaa
     5101 tcccgatctt ctgaatcagt aaacaataaa gttgatatta gcattttaat tgaagatact
     5161 ggtattggaa ttgaagaaga agatcaaaat agaatctttg aggcttttac acaaagtaga
     5221 aagcaagacc aaagtaaata tggaggaact ggtcttggct taacaattgc tcagaagtta
     5281 acaaatctta tgggaggaca aatatttcta gagagcgaaa aaggaaaagg gacaaagttt
     5341 tggattgata tggagaatat tgaaatatac aatcattctt ctaatatttt agaaaatgta
     5401 aatgttttac taggcaacga gaataaacaa tttgagaaat caaaaatact aatagtagat
     5461 gatttaccaa ttaatagaaa attaatttat tcttatctaa aagaatttaa agaactggaa
     5521 ttgtttgaag caacgaatgg ggaagaagca attcaagtag tcaataaaga aaagcctgat
     5581 attattttga tggatattcg aatgccgata ttaaatggat atgaagcagc aaaaattcta
     5641 aaatcgaaag acgagacaaa agatattcct attatagttt taaccgcatc tatatctgaa
     5701 aatacaaaga atgaaattat ggatatttgt gaaggttata tacaaaagcc aattcaaaaa
     5761 aaagtcttaa ttggacaatt gcatacatat cttaaatcaa aatttattca gacgaaagaa
     5821 gtaaaactgg atgaaagtag tatagaagaa aaagtggaca ttgaaaaaaa taaagttcta
     5881 tttaaaatat taaaagaaga aaaagttcaa caatggcaaa ttcattctaa gatacttgat
     5941 atgcaagaaa ttcaactatt cgcagaagaa attgaaaatt tggctctaga atattattca
     6001 cctgatataa gaaaatgggc atccgaatta aaaagaagtg ttaaaaattt tgaaattgat
     6061 acaatgaatg aattattgaa aagtttctta caaattatag agaatctaaa aagtaatata
     6121 gataaacatg acaataaaaa ttgaatatga aagaaaaaat tattttaata gtagatgata
     6181 ctcagaaaaa tattcagctt ttagggcaaa ttctgactcc ggtcggatat agaatcattg
     6241 tagcaatgaa cggcatacaa gcgttaaatg cacttgaaat tcaaaaacca gatataattc
     6301 taatggatgt taatatgcct gaaatggatg ggtttgaagc ttgtaagaga ataaaagaaa
     6361 atgatttatt taaagatatt cctattattt ttttaacagc cagaacggat tcaaatgata
     6421 ttatgcaggg cttcgaatat ggaggagcag attatattac gaagccattt aattcaaaag
     6481 agctattgat tcgaatttct tatcaattag aattaaaaga ccaaagagag gagttaattc
     6541 gtgtaaacac tgaaaataaa caattgctaa gagtcttgtt gcatgatctc agaaatcctg
     6601 tgggcgcaat tgacgttatg tgtgaaatgc taaaacaaaa ttctgaaaat aaagataaaa
     6661 ttattaatct tatctcgcaa gcaacaaaga attctatgag tattttagaa aatgtaaaga
     6721 caatgttcag tgttggagat aagtatattc caaatttggt tttatttaat gtgaaatcta
     6781 caatagaatc ggctttgcga atatttgatc atcaatttaa ttctaagaat atctctctag
     6841 aaataaatat tcctgacaat ttaaaagctg tagtagatgg aagttctttt actgattctg
     6901 tttttaataa tataattacg aatgctataa aattttcatt tccaggttct aaggtgatag
     6961 tttcagcaga agaaattgac gacaaagtta aattaacagt tcaagactat ggaatgggaa
     7021 ttcctccaaa aatattggaa aatatttttg atatttctaa gcctacgagt agaataggaa
     7081 ctaacggtga aaaagggact ggatatggaa tgcctctagt aaaaaaattt gctgaacttt
     7141 ctaaagcaaa aatagaaatt cattctattg aaaaacccaa tgaagggcat ggaactaggg
     7201 ttagtcttta cttagaaaaa aagatttagt ctatttattt taatttactt ttttatattc
     7261 attatatttc cataaaggaa tggaagttat aaaaaataaa atcgaattaa atcaaaggat
     7321 tagaaaactt aaactagaag gaaagaaaat cgcctttgct cccaccatgg gttttttaca
     7381 tgaaggacat atagaattat ttaatgagtg taaatccaat gcagatatat ctgtaacatc
     7441 tatatttgta aaccctgcgc aattcaatga tccaaaagat tttgaaaagt atccgagaaa
     7501 tacagattct gatctaaaaa agtgtgagaa agctctcatt gatattgtat accttccaga
     7561 ggttaatgat atttatccac aaggtaaagt tcctgatata aatatccgaa ttcctcattt
     7621 aatgcgcaat ctttgtgcta caactagacc tggtcatttt gaaggtgttc ttctagtaat
     7681 atccaatctt tttcatattg ttgaaccgga tattgctttt tttgggaaaa aggattatca
     7741 gcaatacttg attataaaag aatttgcaaa ggtaactggg atgaatatcg acgtgatagg
     7801 tgtagaaacc attcgagaaa aagatgggct tgcgatgagt tctagaaatg caaggttgag
     7861 ttcagaggaa agggaggcgg cgacgttaat tcctagagct ttccaactat gcaaagaata
     7921 tatttatgga aaaggaagag atcctaaaaa aataaaaagt attttctctg aggtaattct
     7981 gagttcttcg aaaatgagga tcgattatat tgaagtttta aatgcaaata atttaggtga
     8041 tttggaagtc ttaacgggag aaatactaat tgctgttgca atcttttgtg gagaagttcg
     8101 tcttattgat aatattcgat tgacacttag ctaacttatc ctaagatttc tgctatggaa
     8161 ataatcatcg agtgtattct aaatcctgga gttccctgtg atagtatttt aaattatacc
     8221 tatgttctct ggtttgtatt tggtatcgtt ttaattgttt tagaattttt tattccagga
     8281 gtttttatta tcttttttgg gctaggtgca ttagttacta gcttatttca ttatttattt
     8341 atactttcct ttggaatgca aataactact tggatagtag cagcatttgc gataatgtta
     8401 tttggggcaa aatttttgaa agatttattt ccttctgaag agtcatatga accaactatg
     8461 aaagagagtg t
//
LOCUS       DAOQOS010000187         8469 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_4587_length_8469_cov_4.855717, whole genome shotgun sequence.
ACCESSION   DAOQOS010000187 DAOQOS010000000
VERSION     DAOQOS010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 8469)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8469)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8469
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4587_length_8469_cov_4.855717"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(100..1521)
                     /gene="argH"
                     /locus_tag="PLF66_24330"
     CDS             complement(100..1521)
                     /gene="argH"
                     /locus_tag="PLF66_24330"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016548646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HMZ67237.1"
                     /translation="MQQEKSQKLWGGRFDTKAASIMERIGESISFDQRLYKQDITGSI
                     AHAKNLLKIGILSKEEFENIEKGLKQIQAEIESGKFVYTTELEDIHMHIEHRLTELIG
                     QAGKKLHTGRSRNDQVAQDVRLFIRDESKEVIELILRLLDVILNKAKNSIDIVMPGYT
                     HLQVAQPIRTSHYLLSYFWAFQRDLEQILFTLSVNDILVLGSGALAGVNYNTDRELIQ
                     KELNFGALSQNSIDAVSQRDHVLNFLFAASLILVHASRLCEEIIIYSSVEFGFISLPD
                     SLTTGSSIMPQKKNPDIAELIRGKSARVISNLNHLLVLLKGLPLAYNRDLQEDKISLF
                     DSVDQVKISIEGITEMIREMKFNSEKMQGSLHRGFATATDLADFLVNEKKVPFREAHE
                     LVGRLVSVCVQKNKTLLDVSEEDRGSVTEFFKGEEYLEAISLVKSTEKKNVFGGTARE
                     RQLEQIRNAENSLANLRQRLQVE"
     gene            1572..1943
                     /locus_tag="PLF66_24335"
     CDS             1572..1943
                     /locus_tag="PLF66_24335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67238.1"
                     /translation="MTQIPILFFSLFLFFIHPLAADTGFKPIVLGAIAIFIGFFLILG
                     LGIAGIHRWFANKVFLLSISYKKSFLYTILGSLPGIFVIVMIEKNSYSEFLFLKSIFI
                     SMFFCPLLMFIIQKILKKEGL"
     gene            1943..2242
                     /locus_tag="PLF66_24340"
     CDS             1943..2242
                     /locus_tag="PLF66_24340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67239.1"
                     /translation="MQQVFGLLLGMLFLVFISFVLSVGLISVLSYSYFYFKYKSWKYY
                     KRCMLFTFLGGCLGVFITFVLIPKTQEGQILPYSILFLSMFLSGFASVAVKKFET"
     gene            2261..3385
                     /locus_tag="PLF66_24345"
     CDS             2261..3385
                     /locus_tag="PLF66_24345"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HMZ67240.1"
                     /translation="MNQTIQFLETIIEQGTQLQNDLWTNQSIRLINKVAIFVAILTIP
                     YVILTYILGLYIATIVDCIAIFNYFIIYFINANARFKISRIILLVQGNLHILAISLIL
                     GTESGIHHYLYAAVIAPLFFFREEEFKQIIGFCLFSIFLGMCYPFISDLYSPYSANKD
                     TIKFFFHIPTISGVFIAVLGFILYFYNESFRTQKILDDQNKQLQFIGETDHLTQIGNR
                     RKLESVIRMEWSRGLRKKYTVCVILIDVDSFKLYNDIYGHLAGDECLRSIAKTLKTEI
                     RTDFDFIARFGGEEFIVILHDTDLENAKVVASRMQSAVFNLNILHTKNLEYGRVTISM
                     GIACAIPTLDRSPLELIDEADQYLYEAKTSGKNKFIAQAN"
     gene            complement(3393..3821)
                     /locus_tag="PLF66_24350"
     CDS             complement(3393..3821)
                     /locus_tag="PLF66_24350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67241.1"
                     /translation="MNQTITMPDNWNLVFRDELLLGEGLYSTTFEDTESFFEINLNYI
                     PSLEKFYIENVQLKGDKEILKNKNYSTFARTEADAMLRAKEMACFINQFILKPTVVTL
                     FKQIPLHIISIDDDFHFLGKNQMDKAANNPNYCENLYSAV"
     repeat_region   4102..6091
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=4176..4211
                     /rpt_unit_seq="gtttcaatccactctcttcaattaagaagagagaaa"
     assembly_gap    6093..6192
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     repeat_region   6193..8468
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=6193..6215
                     /rpt_unit_seq="gtttcaatccactctcttcaatt"
BASE COUNT         2851 a         1416 c         1307 g         2795 t
ORIGIN      
        1 gtttgaaagg agtcatcttt acaaaaaaga atctgggtga aaaaaacaaa caatagaata
       61 gttttaaaaa atctcatcga tacctctaaa ttaaatatat tattcaacct gtagacgttg
      121 gcgtaagtta gcaagcgaat tttctgcatt tcgaatctgt tcgagctgcc gttctcttgc
      181 cgttccacca aaaacattct ttttctctgt tgattttaca agagaaattg cttctaggta
      241 ttcctcgcct ttaaaaaatt cagtaacact tcctctatct tcttctgaca catccaaaag
      301 agtcttattc ttttgcacac agacagagac gagtcttcct acaagttcgt gtgcttctct
      361 aaaaggaact ttcttttcat tcactaaaaa gtcggctaag tctgtagcag tcgcaaatcc
      421 tcgatggaga gatccttgca ttttttcaga gttgaatttc atttcgcgga tcatctctgt
      481 tatcccctcg atcgatattt tcacttgatc aacggaatca aaaagagaaa tcttatcttc
      541 ttgcaaatct cgattgtagg cgagaggaag tccttttaat aaaacaagca gatgatttag
      601 atttgaaatc acacgagctg actttcctcg aatgagttct gcaatatcag gatttttttt
      661 ctgaggcata atagaagaac cggttgtaag agaatcaggc agagaaataa atccaaactc
      721 aacagaggaa taaatgataa tttcttcaca aagtctagag gcatgcacca gaataaggga
      781 agctgcaaac agaaagttta agacatggtc tctttgcgaa actgcatcaa ttgaattctg
      841 acttaaagca ccaaagttta attctttttg aattaattct ctgtctgtgt tgtaattgac
      901 accagctaac gcaccagaac caagaaccaa aatatcattt acagaaagag taaaaagaat
      961 ttgctctaaa tctctttgaa acgcccaaaa ataagagagt aaataatgag aagttcgaat
     1021 tggttgtgca acctgaagat gagtatagcc aggcatgaca atgtctatac tattctttgc
     1081 tttgtttagg ataacatcga gtagccgtag aatcaattcg atgacttctt tggattcatc
     1141 tcgaataaaa agtctcacat cttgtgcaac ttggtcattt ctagatcttc ctgtatgcaa
     1201 tttttttcct gcttgcccga tgagttcagt caagcgatgt tctatatgca tatgaatgtc
     1261 ttctagttca gtggtatata cgaatttacc ggactcaatc tctgcttgaa tctgttttaa
     1321 tcccttttca atattctcaa attcttcctt ggagagaatt cctattttca gtaaattctt
     1381 agcatgagca atactgccag tgatatcttg tttataaagt ctttggtcaa aactaattga
     1441 ttccccaatt ctttccatga tggaggcagc ttttgtgtca aatctaccac cccagagttt
     1501 ttgtgatttt tcttgttgca tagattaact gtgaatttaa aaattgattg tttgtcaagt
     1561 tcggaaactc tatgacacaa atacctattt tatttttcag tttattcctt ttttttattc
     1621 atccgttagc cgcagatact gggtttaaac ccattgtatt aggagcaatc gcaatcttta
     1681 taggtttttt tctaatacta ggcttaggaa tagctgggat tcatagatgg tttgcaaata
     1741 aagtctttct tctttcaatc tcttataaaa aaagtttttt atatacaatt ctaggttctc
     1801 tacctggaat ttttgtgatc gttatgattg agaaaaatag ttattccgaa tttttatttt
     1861 taaaatcgat ttttatctcg atgttttttt gtcctttatt aatgtttata attcaaaaga
     1921 ttttaaaaaa agaaggtttg tgatgcagca agtgtttggt ttacttttag gaatgctatt
     1981 tcttgttttt atttccttcg ttttgagtgt ggggctaatc agtgttctct cctattcata
     2041 cttttatttt aaatacaaat cttggaagta ttataagaga tgtatgctct ttactttttt
     2101 aggaggatgt ttaggagttt ttattacctt tgtgttaatt ccgaaaaccc aagaaggaca
     2161 aatcctgcct tattctatcc tttttctatc tatgtttctc tctggatttg caagtgtagc
     2221 agtaaaaaaa tttgaaacct aatctaaata caaatttaaa atgaatcaga ctatacaatt
     2281 tttggagaca atcattgagc aaggaactca attgcaaaat gatctttgga ccaatcaaag
     2341 cattcgactt atcaataaag ttgccatttt tgttgcgatt ctaacaatcc cctatgtcat
     2401 actaacgtat attctgggac tttatattgc gacaattgta gattgcattg cgatttttaa
     2461 ttatttcatc atttatttta taaatgcaaa cgctcgtttt aaaatttcta gaataatatt
     2521 actggtccaa ggaaatcttc acatccttgc gatctcgctg atactgggaa cagaaagcgg
     2581 aattcatcat tatttatatg ctgcagtcat tgctcctctt ttcttttttc gagaagaaga
     2641 atttaaacaa atcatcggtt tttgcttatt ctctattttt cttggaatgt gttatccttt
     2701 tatcagtgat ctctattcac cctactctgc aaacaaggat actatcaaat tcttttttca
     2761 tattccgact atctctggag tttttattgc agtgcttgga tttattttgt atttttataa
     2821 tgaatccttt cggactcaga aaattctaga cgatcaaaat aaacaacttc aatttattgg
     2881 agaaacagat catttaacgc aaataggaaa tagaagaaag ttagaatctg tgattcgaat
     2941 ggaatggagc agaggattaa gaaaaaaata tacggtatgc gtaatactca tcgatgtaga
     3001 ttcctttaaa ctttacaatg atatctatgg tcatttggct ggagatgaat gcctaagaag
     3061 catcgctaaa actctaaaaa cagaaattag aactgacttt gattttattg cccgatttgg
     3121 aggagaggaa tttatcgtca tcttacatga cacagatcta gaaaatgcaa aagtagtagc
     3181 atctagaatg caatccgccg ttttcaattt aaatattcta catacaaaga atttagaata
     3241 tggaagagtt acgatcagta tgggtattgc ctgtgcgatt cctaccctcg atcgttcccc
     3301 actcgaacta atcgatgaag cagatcagta tctctatgaa gcgaaaacct caggcaaaaa
     3361 taaattcatc gcacaagcaa attaaatttc tactacactg ctgaataaag attctcacag
     3421 taatttggat tattggctgc tttatccatt tgattttttc ccaaaaaatg gaagtcatca
     3481 tcaatggata taatatgcag tggaatctgt ttaaaaagag taaccactgt tggcttcaaa
     3541 ataaattgat tgataaaaca agccatctcc tttgctctaa gcatcgcatc tgcttctgtt
     3601 cgggcaaacg tagaatagtt tttatttttt aaaatttctt tatctccttt taattgcaca
     3661 ttttctatat aaaacttttc tagagaagga atataattca gattgatttc aaaaaaactt
     3721 tctgtatctt caaaggtagt gctgtaaagc ccttccccta aaagcaattc atccctaaaa
     3781 actaaattcc aattgtctgg cattgtgatt gtctgattca tatttatctc cgtaattact
     3841 taatattttt taagtagtat tttgtaaagt atgtaatcga aggaataaat actataaaaa
     3901 gtgacttttg tttcattctc gcttcccagc aggttcttac tttctttctt tttttagtaa
     3961 gaagattttt ttttaatatg tgttggtttt atgattcttt ccaattcagt gttactctat
     4021 tgctattgtc tttttatgaa aattttttct aattacaaag cccccttttg caaaggtgta
     4081 aggggattcg aatgaatcta tatttattcc aactctcttc aattaagaag agagaaatca
     4141 aaaaacaaga gattgccaaa gagctaaaaa ctcttgtttc aatccactct cttcaattaa
     4201 gaagagagaa atagtcttgc agaagtccga caacaaatta ttttctgggt ttcaatccac
     4261 tctcttcaat taagaagaga gaaatgattt caactctcgt tcaacaactg acatgaacag
     4321 tttcaatcca ctctcttcaa ttaagaagag agaaatcgtt tgatactttt cgacaaggaa
     4381 gaaaatgttt tgtttcaatc cactctcttc aattaagaag agagaaattt tataatctaa
     4441 gcatgaaaat tccttttata ggtgtttcaa tccactctct tcaattaaga agagagaaat
     4501 catttagtta ctaattcaaa aatgaatcca aaaaaagttt caatccactc tcttcaatta
     4561 agaagagaga aataaaactt cccctagaaa tttgtcccct tctaggctag tttcaatcca
     4621 ctctcttcaa ttaagaagag agaaataaaa aataatattg ctagtattgc agaccaaaaa
     4681 ggtttcaatc cactctcttc aattaagaag agagaaatat tttcagcaaa gagggcaatt
     4741 ctttttaaat cgcaagtttc aatccactct cttcaattaa gaagagagaa atagataaaa
     4801 ttcatgcaac aaaaattaat gatttagagt ttcaatccac tctcttcaat taagaagaga
     4861 gaaatttctt ctgagtaaag gaaaggaagt tttaaattag tgtttcaatc cactctcttc
     4921 aattaagaag agagaaatcg ttgcattttt catgagttgt tgcattaaaa cgtttcaatc
     4981 cactctcttc aattaagaag agagaaatca tttttaacct cgaaaatcat ttcgtttttt
     5041 cctgtttcaa tccactctct tcaattaaga agagagaaat atatttcatc ttcattttct
     5101 ttgaattcaa agcactgttt caatccactc tcttcaatta agaagagaga aatctataga
     5161 tgatttgtct agtttgcttc tagcagaaat gtttcaatcc actctcttca attaagaaga
     5221 gagaaataag aagctaacag gcttaaagaa caaataaaaa ttgtttcaat ccactctctt
     5281 caattaagaa gagagaaata tatttcattt gtatcatctt tgaattcaaa gcactgtttc
     5341 aatccactct cttcaattaa gaagagagaa atagcagtta ggtcattgcc accgtatgca
     5401 gtatcgaggt ttcaatccac tctcttcaat taagaagaga gaaattagac tttaatatgt
     5461 ttgagaattt cgacattgaa gtttcaatcc actctcttca attaagaaga gagaaatcaa
     5521 agatttactc aaatcatacc aactactaga cagtttcaat ccactctctt caattaagaa
     5581 gagagaaata ttctgagtgt ataggagttt acccatttgt ttttttgttt caatccactc
     5641 tcttcaatta agaagagaga aataaataga gcaggcttat ctctatcaac aagatcagtt
     5701 tcaatccact ctcttcaatt aagaagagag aaataataga gcaggcttat ctctatcaac
     5761 aagatcagtt tcaatccact ctcttcaatt aagaagagag aaatgtccag caataaagct
     5821 aaaagttatg gcgagcgtaa agtttcaatc cactctcttc aattaagaag agagaaatga
     5881 caacctataa tttacgataa gaattcatta atgtttcaat ccactctctt caattaagaa
     5941 gagagaaata tttatcaata tggttcttgc ttatttctac aaagtttcaa tccactctct
     6001 tcaattaaga agagagaaat aggtttgttt ttacatattc gtttccgtat ggattgtttc
     6061 aatccactct cttcaattaa gaagagagaa atnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6181 nnnnnnnnnn nngtttcaat ccactctctt caattaagaa gagagaaatc tgttgcattg
     6241 taaggtgttt cggagccttt cagagtttca atccactctc ttcaattaag aagagagaaa
     6301 tcgaggcggt aaaggcattt atcatagatt tgatataggt ttcaatccac tctcttcaat
     6361 taagaagaga gaaattatat agtcgttagt tctaaaggtg ttgtaggcta tgtttcaatc
     6421 cactctcttc aattaagaag agagaaataa caaacaaaga gacgatagac gtgatagcga
     6481 gcttgtttca atccactctc ttcaattaag aagagagaaa ttgccattta ttgaccctat
     6541 aacattagtg caatatgggt ttcaatccac tctcttcaat taagaagaga gaaatctttt
     6601 ttatttacga taggaagtcg aaccgaaaaa gtttcaatcc actctcttca attaagaaga
     6661 gagaaatgaa aatagattaa gccctgtatt gtctttctta caagtttcaa tccactctct
     6721 tcaattaaga agagagaaat ctataggtat cttaggcaag acgttaatct tgttttgttt
     6781 caatccactc tcttcaatta agaagagaga aataaggcgg aaagtatacc tttatgaagg
     6841 aattatcatt gtttcaatcc actctcttca attaagaaga gagaaattct atctgaagta
     6901 gcagaaaaaa atatcacaac ttttgtttca atccactctc ttcaattaag aagagagaaa
     6961 tggtttaaat ataatccaga atgcagcatc aaggggtttc aatccactct cttcaattaa
     7021 gaagagagaa atcgcaagtt cctaattttg tttttgatgt tttgatgttt caatccactc
     7081 tcttcaatta agaagagaga aatgatgagg tagaaacttg gaaacaagtt gcacaaatgt
     7141 ttcaatccac tctcttcaat taagaagaga gaaattacat tggatgttcc tgtaaatctt
     7201 gtcgaaaatg gtttcaatcc actctcttca attaagaaga gagaaataaa aatacaaaat
     7261 taactctatc tgaagtagca gagtttcaat ccactctctt caattaagaa gagagaaata
     7321 tctggtatgc gtgacaagtg gcaagcaata gctaatgttt caatccactc tcttcaatta
     7381 agaagagaga aattaattct gaataacccg aaatgaccat gaagtgacct gtttcaatcc
     7441 actctcttca attaagaaga gagaaatgtt tgggaaaaag aatattatcc agatattctt
     7501 agtttcaatc cactctcttc aattaagaag agagaaataa aaatttggaa tgactaaatt
     7561 gcaatttaaa cttttgtttc aatccactct cttcaattaa gaagagagaa attgtaaatt
     7621 aacaaaatgc gaagttgaag aaacgaggtt tcaatccact ctcttcaatt aagaagagag
     7681 aaatgagatt ggtttattgc agatttagag cttgtagatg tttcaatcca ctctcttcaa
     7741 ttaagaagag agaaataaga tgctgatcta tttagatttt tgcatgaatt agtttcaatc
     7801 cactctcttc aattaagaag agagaaatcg ataatattag agcaatcgag caagatttaa
     7861 aagtttcaat ccactctctt caattaagaa gagagaaata ggtagaattc tagataaaaa
     7921 tattatgagc ttagtttcaa tccactctct tcaattaaga agagagaaat aaataaccgg
     7981 atactaaaaa cagaattagt caattggttt caatccactc tcttcaatta agaagagaga
     8041 aattaagagg tttcaggggg tgatataata tgcctgctaa gtttcaatcc actctcttca
     8101 attaagaaga gagaaataat agaatcctgt ctaattaagt cacgagtctg caaagtttca
     8161 atccactctc ttcaattaag aagagagaaa tatttgataa atttaaaatt ctcgattcat
     8221 ggacagtgtt tcaatccact ctcttcaatt aagaagagag aaataaagaa gttatatcag
     8281 agcaactttc gataataagt ttcaatccac tctcttcaat taagaagaga gaaatatttg
     8341 tcaaataaga ttagagaggc atatttaaaa ggtttcaatc cactctcttc aattaagaag
     8401 agagaaataa acgaagtaga aacagcactt gaacttgcca ttaatgtttc aatccactct
     8461 cttcaatta
//
LOCUS       DAOQOS010000188         8311 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_4718_length_8311_cov_5.656977, whole genome shotgun sequence.
ACCESSION   DAOQOS010000188 DAOQOS010000000
VERSION     DAOQOS010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 8311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8311
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4718_length_8311_cov_5.656977"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            637..2310
                     /locus_tag="PLF66_24355"
     CDS             637..2310
                     /locus_tag="PLF66_24355"
                     /inference="COORDINATES: protein motif:HMM:TIGR04456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LruC domain-containing protein"
                     /protein_id="HMZ67242.1"
                     /translation="MIRNTTIIAVIVFSMLFTQCENKKKKNYIPIQSIANIGSSANPS
                     TSTGTPATNIGNTQAVVGTEYGVETFSYQTTRSVTIDATVVDGDDKAIVGALVEIKDG
                     ANAVSIYQQLSNSNGKVVGSLTLNTTETKINVYVTYNGFTTAATAVAIKQENKVLIKI
                     TTIKVPIKTSELQLATASSGATSVSLVDTDGDGVLDQFDDYPNDVTKATKLRFPASGV
                     NTIGFEGTFPSAGDADLNDLVTVYYIEEDQNAAGQIVEIRGSFQNMAHGAGNQYSLNL
                     RLPSTVNITYESEVRDGNNVLQPTAAPTKSKTEATGISQYTPSATDLQDGLRILGNTT
                     TTVGATWYTKNTDTTYAPGYMAKIKIKFLTPVDRATIGSAPYDTFVRVETKAIATTPG
                     YPTEGPRSVTTASNFDVYEIHLPGKYKYTTTATVDGVTRNVGEDIYIDKNGFPFAVIV
                     PGLWKWPAESKDLRVAASSGYPRFATWVSSAGQTDMDWYNDASAATSPNVYYSFVTTN
                     RAALDTPSSQLLAFIGGVTLLQYQGMLLGIVILISLLLFYRRTHTPKQI"
     gene            3276..5546
                     /locus_tag="PLF66_24360"
     CDS             3276..5546
                     /locus_tag="PLF66_24360"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HMZ67243.1"
                     /translation="MKKLAIILLFLSFLFSNCLPHKRKAPEAIKGVLDLRYVGETRDL
                     WDFENDGIVDLNGEWEFYWNEFRNFEPCKDGQNCSPPKEPFYIKVPAAWNNFKIDGKT
                     VGGFGFATYRLRILLPPSDEKSKSRLKQNLSLRMPWEYTAYKLYVNGELLSEVGRIGK
                     NEEESIPRFYPQALDIPSIDFNSLDLVIHISNFHHSSGGGLWDTLSIGEKNSVHSMRE
                     KSLSMDFFLAGSFLIMGCYHLGLYWLRKKDRSTFYFGLYCLLLSLHSLLSGECFLYQL
                     LPSLSFQSGVKLNYLSFYVGTPVFIIFLKHAFSEDVNDLVIKFIVYPSFLLSLIVLFF
                     PSQIFTATLVAMHVIILISCLYTIYLMFLCSYRNRVGAKSFLIGILVFTLFVIDDILF
                     VNGVIDFGGQYGAIGFFWLIFFQSFVLSIRFSSAFVRIEDLSENLENQNRILQEQSTK
                     LENQNTLLKKLTSEIFETNAAYEKFFPSQFIQLLEKDHITKLTLGDQMGREMSVLFSD
                     IRSFTTLSESMSPKENFDFINGYLSRIGPFIRKYNGFIDKYMGDNIMALFDKNPNHAV
                     DAGIEMLEELYRYNQVRMSKGRIPISIGIGIHTGNLTLGIIGEAGRMEGTVIADAVNL
                     ASRLEGLTKMYGASLLISEDMLNKMSNIEKYQFRKVDTVKVKGKNTAIAVIEIISGNS
                     ERITNLKLNTKKNFEEGVKRYLERDFSEAVKRFAQVLEKDPLDKAAKIHFERSLYYDK
                     HRVPPNWEGVEAMETK"
     gene            complement(5957..>8311)
                     /locus_tag="PLF66_24365"
     CDS             complement(5957..>8311)
                     /locus_tag="PLF66_24365"
                     /inference="COORDINATES: protein motif:HMM:NF019224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1554 domain-containing protein"
                     /protein_id="HMZ67244.1"
                     /translation="LIFTTSNWNTAQVITVVGVDDLIVDGDKVSLLSFSPSVSNDKEF
                     DGLSITPLSITNKDDDIPGFTITPTSGLITSETATTVNFTISLTSEPKADVTIPSITS
                     SNTAEGTVSISTITFTSTNWNIPQTITATGVDDILSDGNQTYQIQFSAASSSDPVYNG
                     KIATSVSIINTDDETFGVTVTPTAGLTTTEVGGTASFNIVLNSASTSSVTIPISSSNT
                     AEGTVNTSSLTFTPGNWNVPQTVTITGVNDFVADGNTSYTINVGPCSSANSNYQGLVP
                     SSVSVTNNDNDTAGYNVVSGTLMVTDAGQLSSSFTIQLTSQPVQPVTVPMVSSMPTEA
                     TVSPSSIVFTSSNWNVAQTVTVSGVSDGISDGNKAFTIDFQSAVTTDTAYNGLGMPGS
                     INAGSCDNDYGGVSIITACNASPNQNTTKQGATATYYLILSQAPTANVVVPIVSANTN
                     QGTVSPASVTITSANWNQFAVSNQFVVTGFNNNLYDGDVTYTVNINAATSADPIFNGI
                     DPVDITLVNRDTKNYFNISAISGNTTEAGGTANFSISLLGAITSNVSFNISSNNTAEG
                     TVSPASMTFTSANWSTPQPVVVTGVDDSIADGNTTYAINMTAATGDPIYAGRTPASVN
                     VINTDVGEKRTFITTNPTNGMLGGIAGADAICNADPAKPSITPNNYKALIAISGTRTG
                     APLTGWVLAANTKYFLTDGTTLLFTTNASSIFTFGTFNAAFSGTSYWTGLTNAWGPNG
                     SHCTNWTTSAGSGQIGDGTATNSASIANTTALCNTTHSLICVQQ"
BASE COUNT         2721 a         1423 c         1486 g         2681 t
ORIGIN      
        1 agtcagaatt tattttcaat cgtgctataa gattttcctt ttttgcttag ttttaaaatt
       61 aaataaaatc gatttttaaa aattttactc tttttactca accaaattaa acctatgtaa
      121 gctatcgact caatagaatc cttagcaatc acaatcttcg gtagaaaata ataggaaaaa
      181 attttgaaat tgaatgagaa gatccaatgg attaaacttt ttttcttaat taaaatcaga
      241 tcatctgtat tcaaaatctt tcctcatttt tttatctaca aaagaaaggt ttaaatcaaa
      301 actagagcgc ttacctttca agaagaatgc tttaaacgaa actagtgaaa gtttactaaa
      361 aaaatatttt ttaagataaa aataaatatt ttcaaaccct ttcttttgat caaaataaga
      421 tggaaagcat gtccgagaag gtgatttctt gaatcttcaa ttgggtttca aagcaaaaaa
      481 gtatttttct gagggttctc tgatgtatca tccttcctac tattttgtta ttttttttaa
      541 aaaatcgaat tttttttgcc aagaaaatat ttgcttttta taaattagta attattctta
      601 caccctattc gtttaattag ataggagaag agaaaaatga tacgaaatac aacgataatt
      661 gctgtgatag tctttagtat gctatttaca cagtgtgaga ataaaaagaa gaaaaattac
      721 atcccgattc aaagcattgc aaatatcgga agttctgcaa acccatctac ctctacggga
      781 acaccagcaa ctaatattgg aaatacacaa gctgttgtag gaacagagta tggcgttgaa
      841 acttttagtt accaaacaac tagatctgtt acaatcgatg caactgttgt cgatggagat
      901 gataaggcaa tcgtaggagc tttggtcgaa attaaagatg gagcgaatgc ggtttcaatt
      961 taccaacaac tttcaaattc aaatggaaaa gtggttggat ccctcacatt aaatactaca
     1021 gagactaaaa taaatgttta tgtgacttac aatggattta caactgctgc aacagcagtt
     1081 gcaattaagc aagaaaataa agttttaatc aaaattacta caattaaagt gccaataaaa
     1141 acttctgaat tacaacttgc tacagcttct tctggggcaa ctagtgttag cctcgtagat
     1201 actgatggag atggggtttt agatcagttt gatgattatc caaatgatgt tacaaaagca
     1261 acaaagctaa gatttcctgc aagtggagtg aataccattg ggtttgaagg aacctttcca
     1321 agtgctgggg atgccgatct aaatgactta gttacagttt actatatcga ggaagaccag
     1381 aatgcggcag gacaaattgt agaaattcgt ggatcttttc aaaatatggc acacggtgca
     1441 ggcaaccaat attctctcaa cttacgcttg ccttcaactg taaatatcac ttatgaaagt
     1501 gaagttcgtg atggaaataa cgttctgcaa ccgactgctg ctcctactaa gagtaaaacg
     1561 gaagcaacag gtattagcca gtatactccg agtgcaactg atttacagga tggacttcgt
     1621 attttaggaa ataccactac tactgttgga gctacatggt atactaaaaa cactgacact
     1681 acctatgcgc ctggatatat ggcaaagatc aaaattaaat ttttaactcc tgttgatcga
     1741 gcaactatcg gttcagctcc ttatgatact tttgtccgtg ttgaaacaaa agcgattgcg
     1801 actaccccag gttatccaac agaaggacca agatctgtta ctactgctag taactttgat
     1861 gtatatgaaa tccatcttcc tggaaaatat aaatatacaa caactgctac tgttgatggt
     1921 gtaacaagaa atgttggcga ggatatatat atcgataaaa atggatttcc ttttgcagtg
     1981 atcgttccag gattatggaa atggccggct gaatctaagg atctaagagt agcggctagt
     2041 tctggttatc cgagatttgc aacatgggta agcagtgctg gacaaacaga tatggattgg
     2101 tacaatgatg cttcagcggc tacaagtcca aatgtttatt attcctttgt gactacaaat
     2161 cgtgcagcac tcgatactcc aagtagccaa ctcctagcat ttattggagg tgttacattg
     2221 cttcaatacc aaggaatgct cttaggaata gtgatactta tttccctact cttgttctat
     2281 cgaagaactc acactccaaa acaaatctaa atagatttta catttctcaa agccaaaaac
     2341 catcttggct ttgagaattt cttatcaaat cctttctatc taattcaaat ctaattccta
     2401 attttctagc tagtagaaat cgagcgatga ttgaataaga aatattcaat ctttttctta
     2461 tttttattat ttaaatcaac ttacacaata tgagccaaat gtttaaaatg agatttgttt
     2521 ctagcggagg gcttatccac tgagcgtcac gctctccagc agccgaacta tcgcaggcga
     2581 atgatattat tagtttttta acattctata atattttcaa aatcatcatt tgctaccaaa
     2641 aataacaatt ttgcgtaaca ttagtaattt gaaataaagt gttgataaat aattaaatcg
     2701 aatgattcat tttgttattc aaaatcccgg cttgcgatag ttgtgatgaa tccccactta
     2761 gcaaacccag atattgagat tattaaacct gaaaaaaaat attaaaatct tctcttaaag
     2821 taaagaataa acggatgcaa ttataatttc tttatctaaa tcacggcaat cgaatttaaa
     2881 ttgaagaaaa taaatacata ttgcatagag gtaaacggcg agtatccgca ttctatcaaa
     2941 tttaaactga taaaatctaa ctaagaaacc cacatagttg caattaaata taaattaatt
     3001 cataactagt tatttttgct ggtagagaag attggataaa cttttttaaa gaaaaaatat
     3061 agagaaaaaa cttagcattt caatttgctg aatcctccaa aattaattta tttactatgg
     3121 aatttataga cccgttacta gtaatttaac gtaaaataat tcctaatttt attcccccaa
     3181 ttctaaatcg ctaactctaa acttagtatg agtaaaacta ttttttattg aacaatcaaa
     3241 ctattgtcta aaaatgtagc aaatctaggc tcactatgaa aaaattagca atcattttac
     3301 ttttcctatc ttttcttttc tcaaattgct taccgcataa acgaaaggct ccagaagcga
     3361 taaagggtgt tcttgattta cgttatgtcg gagagacacg cgatctttgg gattttgaga
     3421 acgatggcat tgtcgattta aacggagaat gggagtttta ctggaatgag tttagaaatt
     3481 ttgaaccatg taaagatgga caaaattgtt ctccaccaaa ggaacctttt tatataaaag
     3541 tgcctgctgc ttggaataat tttaagattg atgggaagac tgtaggtgga tttggattcg
     3601 caacgtatcg actgcgtatc cttctacctc caagcgatga aaaaagcaag tccagattaa
     3661 aacaaaatct ttctctgcgt atgccttggg aatatactgc ctataaactg tatgtgaatg
     3721 gagaactttt atctgaagtt ggaaggatcg gcaaaaatga agaggaatca atccctagat
     3781 tttacccaca agcattagat ataccgtcta tcgactttaa ttctctggat ttagtaattc
     3841 atatttctaa ttttcatcac agcagtggag gtgggttatg ggataccctt tccattggtg
     3901 aaaaaaattc agtccattct atgcgtgaaa aaagtttaag tatggatttt tttctagcag
     3961 gtagtttcct gattatgggg tgttatcact taggtcttta ttggttacgc aaaaaagatc
     4021 gttctacttt ctatttcgga ctttattgcc tattactttc gttgcactca cttttaagtg
     4081 gagaatgttt cttatatcag ttgcttccaa gtctgtcttt tcaatctggg gtcaaattga
     4141 attacttatc cttttatgtc ggaacacctg tatttattat atttttaaaa catgcttttt
     4201 cagaggacgt aaatgatctt gtgattaaat ttatcgttta cccatccttt cttctatctt
     4261 tgattgttct tttttttcca tcgcaaatat ttacagcaac cctagttgcc atgcatgtta
     4321 ttatattaat tagctgccta tataccatct atttaatgtt cttatgtagt tatcgaaata
     4381 gagtcggagc aaaatctttt ttaattggta ttttagtttt tactctattc gtcattgatg
     4441 atattttatt tgtaaacgga gtgattgact ttggaggtca atacggagca ataggatttt
     4501 tttggcttat cttttttcag tcttttgttc tctctatacg gttcagcagc gcctttgtaa
     4561 gaatagaaga tctttctgaa aacttggaaa accaaaatcg cattttgcaa gagcaatcta
     4621 ccaagctaga aaatcaaaat acattactaa aaaaactcac ttctgaaatt ttcgaaacta
     4681 acgctgctta cgaaaaattt tttccatctc aatttattca attattagag aaagatcata
     4741 tcaccaaatt aacgttaggc gatcagatgg gtcgagagat gagtgtgcta ttttctgata
     4801 ttcgttcgtt cactacactt tcggaaagta tgagtcccaa agaaaatttt gattttatca
     4861 atggttactt gtcaagaatc ggaccattca ttcgcaaata taatggcttt atagataaat
     4921 atatgggtga taatattatg gctctctttg ataaaaatcc caatcatgca gtagatgctg
     4981 ggattgaaat gttggaggaa ttatatcgat acaaccaagt gcgaatgtca aaggggagaa
     5041 tacctatttc tattggaatt ggaatacata cgggaaatct gactttagga attattggag
     5101 aggctggtag aatggaagga actgtgattg cggatgcagt aaatctcgca tcccgtttag
     5161 aaggactaac caaaatgtat ggtgcgtctc ttttaattag tgaagatatg ttaaataaaa
     5221 tgagtaatat agaaaaatac caatttagaa aagtagatac agtcaaagtc aaaggcaaaa
     5281 atacagcgat tgccgtaata gaaattataa gtggaaactc agaaagaata actaatttaa
     5341 agctcaatac aaaaaaaaat tttgaggaag gcgttaagcg atacctagaa agagattttt
     5401 cagaggcagt taaacgtttt gcacaagtgc tagaaaaaga tccgctagat aaggctgcca
     5461 agattcattt tgaacgatct ctttactacg acaaacacag agttccccct aattgggaag
     5521 gtgtggaagc catggagact aagtgagatt atgcctgaaa caagatttgg agaaatttta
     5581 gaaaaagtct tagaaagaaa ctgaaagttt tcctcttttt agaacttctc ttaaatgtct
     5641 agattgaaaa tcttatgaat ttattcacaa atcgaaaaat cattcattgt tagagttaag
     5701 gaatcacaat tactctattt aaagctcgct ccaatgtgca aagattctaa attcaaaaga
     5761 gccaaatctc ttatggaaca tctctttctt tcagtgaacg aaacggagac aaacttatca
     5821 tttaaaacag gattcaaaga tccttaaatg actatcaaac caattccata acctatctgt
     5881 ctagagtgcg tcagtcacta tatcaattgt gtttttcttt ttgattaaac tagtcaaaaa
     5941 tgttttaaaa cttttcttac tgttgcacgc aaattaaact gtgtgttgta ttacaaagtg
     6001 ctgttgtatt tgcaatactc gcagaatttg tagctgttcc atctccgatt tggccagagc
     6061 cagcagatgt tgtccaattg gtgcagtgac ttccgttagg tccccaagca ttggttaagc
     6121 cggtccaata ggatgttcca gaaaaggcag cgttaaatgt tccgaaagta aaaatactac
     6181 ttgcattcgt ggtaaatagg agagtagttc catctgttaa aaagtatttt gtattggcag
     6241 ccaacaccca tccagtaaga ggagcacctg ttctagttcc agagatagct atcagtgctt
     6301 tataattatt cggagtaata gaaggcttag ctggatctgc attgcaaata gcgtccgcac
     6361 ctgcaattcc tccaagcatt ccatttgtag gattcgttgt gataaaggtt ctcttttctc
     6421 ctacatctgt atttataacg ttaacggatg caggagtcct accggcataa ataggatctc
     6481 cagttgctgc tgtcatatta attgcatagg tagtatttcc atctgcgata gaatcatcca
     6541 ctccagtcac aacgacgggt tgaggggtgc tccaatttgc agaagtaaat gtcatactag
     6601 ctggacttac agttccctct gctgtattat tggatgatat attaaaacta acgtttgagg
     6661 taatggctcc aagtaaacta attgaaaaat ttgcagtgcc tcctgcctct gttgtatttc
     6721 cagaaatagc agaaatatta aaataatttt ttgtgtccct atttactaaa gtaatgtcaa
     6781 caggatcgat tccattgaag ataggatcag cgcttgtcgc tgcgttgata ttgactgtat
     6841 acgtaacgtc cccatcataa agattattat taaacccagt tactacaaat tgattcgata
     6901 ccgcaaattg attccagtta gctgatgtaa tcgttacact cgcaggtgaa actgttccct
     6961 ggtttgtatt tgcactaacg ataggaacaa ccacatttgc tgtaggagcc tgagaaagaa
     7021 tcaaataata agtggctgta gcaccttgtt ttgtagtatt ttgatttgga ctagcattac
     7081 aagcagtgat gatagacact cctccataat cattatcaca actaccagca ttaatactcc
     7141 ctggcatacc aagaccgtta taggcagtgt cagtagttac tgcagattga aaatctatcg
     7201 tgaatgcttt attaccatcg gaaattccat cacttacccc ggatacagtc actgtttggg
     7261 caacattcca attggaggaa gtaaatacaa tactagaagg cgagacggta gcttctgttg
     7321 gcatagaact caccattgga acagtaacag gttgaacagg ttggcttgta agttgtattg
     7381 taaaagaaga actgagttgt cctgcatctg taaccattaa cgttccagat acaacattat
     7441 aaccggcagt atcattgtca ttgtttgtga cagatacact cgatggaacg agtccttggt
     7501 aatttgaatt tgcactcgaa caaggtccta cattgattgt ataggatgta ttaccatctg
     7561 ctacaaaatc atttactcca gtaattgtta ctgtttgcgg aacattccaa tttccagggg
     7621 tgaaagttag actagaagta tttaccgtcc cttctgctgt attactagaa gaaatgggaa
     7681 tcgtaacact cgatgtgctt gcactgttta aaacaatatt aaaagaagca gtgcccccta
     7741 cttctgtagt agtaagacct gcggttgggg taactgtcac tccgaaagtt tcatcgtctg
     7801 tattaataat cgatacactc gttgcaatct ttccattgta aacaggatca gaactacttg
     7861 cagccgagaa ttgaatttga taggtttggt ttccatcact caagatatca tctacacctg
     7921 ttgctgttat cgtttgtgga atattccaat tcgtggaagt aaaagttatg gtagatatac
     7981 taacggttcc ttctgcagta ttagaactgg taatacttgg aattgtaaca tctgcttttg
     8041 gttcacttgt aagtgaaatc gtaaaattta cagtagtggc tgtttccgat gtaatcaatc
     8101 cagatgtagg tgtaattgta aatccaggaa tatcatcgtc tttatttgta atcgaaagtg
     8161 gtgttatgct aagtccatca aactctttat cattactaac gcttggtgaa aaactcaaaa
     8221 gagatacttt gtctccatcc acaattagat catccactcc aactactgta atgacttgag
     8281 cagtgttcca attcgatgtt gtaaagatga g
//
LOCUS       DAOQOS010000189         8194 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_4836_length_8194_cov_5.380268, whole genome shotgun sequence.
ACCESSION   DAOQOS010000189 DAOQOS010000000
VERSION     DAOQOS010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 8194)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8194)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8194
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4836_length_8194_cov_5.380268"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1140)
                     /locus_tag="PLF66_24370"
     CDS             complement(<1..1140)
                     /locus_tag="PLF66_24370"
                     /inference="COORDINATES: protein motif:HMM:NF016311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HMZ67245.1"
                     /translation="MLLFYTVLTSLLFPFTLILPLLNKKAKSFLNKRHLDKKKIQDFF
                     LEPNQRVIWLHASSVGELDQCKALAKVIRENEKDTFLLQSVFSDSVLEKNYDTENTNL
                     TFRLPLDFYFSYDFVFKKFSPERLVLMAWDTWPHLVLAANRHNCKVYLACAVLDPKSK
                     RSSFFAKGLTRAVFSRLYAISPVDESMVESFQELSDTKVRIKAFGDSRFDSVYHKIQS
                     KKPNEEFVKFAQKQSQPIFILASTYKDCDSILLPNIENLLSFNFTIWIFPHHINSSRI
                     EEILTSLKKRNLGVSLYSEAKNSCKDRIILFDVLGILAFAYFYGKIAYIGGAMHNKVH
                     NVLEPAFFGLPLITGEKILNSFDAISLKEKGTLKTVNSSKEFLEAV"
     gene            1243..2325
                     /locus_tag="PLF66_24375"
     CDS             1243..2325
                     /locus_tag="PLF66_24375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67246.1"
                     /translation="MPILSFSFLFLLNSLTIHTQEAEDYTKKIEMIWGWEEETKSINK
                     KEINIENPVPVFLSQFQPQKQEFPINPTMENIIKGKAGTKITIPAHSIALPSEFRKGD
                     ILTIQLIEIYNDLDFITSGVSLFYFNPEPNIFESGGMFKLTATYYDKPLQLKRGARLK
                     VEIPSHIKTDKKMNMYRLDERNGWIDKGSVSVEKSSEKIENPASEGPDKKKDGSSESG
                     VEYIPSYIFRAMDEFKWWNADYPNPNRTCIEGTIQTFEANPPYSITSLGIDFKNATTR
                     YADGKKFQLNVIKAKNVKLIAVDERGNIGLISEINTGNLNAFIKEGSKEKCTNVGTIE
                     IKKVDREILKDRGKLLNHLGLKDIVK"
     gene            complement(2356..3219)
                     /locus_tag="PLF66_24380"
     CDS             complement(2356..3219)
                     /locus_tag="PLF66_24380"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HMZ67247.1"
                     /translation="MKQSYRLIDSGDYEKLEIIGGYKIIRPALGSPYKKKDPSLWKNP
                     DAHYLKNDTGSGHWKFYKKIPESFSIEIANLSAKIKLTPFGHIGIFPEQEFNWTLLQK
                     LGGIQNNFEVLNLFAYSGMSTLSAANAGYPVCHVDSSKGMIAWARENAELSNLTDRKI
                     RWIEDDVLKFIKREVKRNRIYKGFILDPPTYGRGAKGEVWKIEDDLIPLIDEMIRLSD
                     GKPEFVILSCHSTGFSPLTLIRILKSFIKEDGFYFSKELFIPEESKENLPGGFCAYFL
                     SKNFSKLKTEF"
     gene            3401..4690
                     /gene="asnS"
                     /locus_tag="PLF66_24385"
     CDS             3401..4690
                     /gene="asnS"
                     /locus_tag="PLF66_24385"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010569976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HMZ67248.1"
                     /translation="MYLYELEQNLNKVITLSGWVHGKRGNNKIQFLQIRNSGRIIQVV
                     CEKEKLGEDVFEKIKALHQETSIELKGLLVVSEKSELGYEIILESYNVVGDAIDYPIT
                     PKEHGPDFLHNHRHLWLRSKKQLSIIQIRNELSFQIRKYFYDRHYILIDTPILTGSVG
                     ESAGTLFETEYFDEGKAYLAQTGQLYLETAIFAHSHVYCFGPTFRAEKSKTRRHLTEF
                     WMLEAENANYRNEDNINLQDDFIRTIVKNTVNACLPHLKTLERNSETILNYVEKPFQR
                     ITYSKAIEVLKEKGEGIEWGEDINAERENILTTHFNGPVFVMNYPRAIKAFYMKQNPE
                     DERTVLCADLIAPEGVGEIIGGSEREENYDKIVERLKEEKLPVENYSWYLDLRKYGSV
                     PHSGFGLGLERLVAWICGLPHVRECIPFPRLMGRLHP"
     gene            4761..5558
                     /locus_tag="PLF66_24390"
     CDS             4761..5558
                     /locus_tag="PLF66_24390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67249.1"
                     /translation="MIQSNTYSSTNLEREAWESFEVGSFEDVINAVEQNPSSALLNHL
                     AILAKYELGNRSNDDLKKFSANGLSVFTPLVAAYNAFYSGNRSLALQKIKEYLEKNNP
                     PICLIIINFAVKVAFSAEAYEDCLKIIYLSEKKTGNPNEFVKERIECYFQLKKHNELI
                     QYFKSVYKLLDSNYDIYLKAGLSLNALGKYKEAEYLFGKIPGKRNLPTYDEKKQEFKP
                     IINKISEFEKKEVLSNSELKDLGFAYLFNAEYKKAEEVFKKVAVAIQ"
     gene            complement(5530..6408)
                     /locus_tag="PLF66_24395"
     CDS             complement(5530..6408)
                     /locus_tag="PLF66_24395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67250.1"
                     /translation="MILRILALILLLLIIPSYANEKSQLIIKDFFDTVVNWSTMQISL
                     NASEKLPRVIIDVDDPEYGKENTAYNISEARNKSLLTAKEKTKIHFVRSIESIRLNEE
                     YTIIEKVNKDEKFRERFNEFFLNESSEIKVKYIQDEVRVESLTKLLGSKGLMNYLMIN
                     YDTEKFPEFMEIPVPEAYTGLIIDARHLDANPALFPRIFTDKGLEIYSMSLVNKNFAI
                     DNGFVIYMSDPIKAMKHQRVGENPYLVVATNVIGKNKTGYTIPTKEATKILATKETKK
                     NLKKCKVIIVLRRQLS"
     gene            complement(6567..7601)
                     /locus_tag="PLF66_24400"
     CDS             complement(6567..7601)
                     /locus_tag="PLF66_24400"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HMZ67251.1"
                     /translation="MKVSIAKNLKHLFSLPKSTLYLLSGKKPIQIDNKTLDPGLQLVT
                     TVLSIFPSFATLAPKDARQVLEALSGLIEAPINHKPEIENIQIAVENPKGKIPLRIFK
                     PRIRTKEKLPVLVYNHGGGYVMGSVDSHEKICEIICENAHCIVVSVGYRLAPEYKFPI
                     PVYDTITAYKWVCDHIEFVEGDRSRIATGGDSAGGTLATVLSHELKNTEYKPFFQFLI
                     YPGTDASRKSDSRYRYGEGFFLTGKQIDWFIENYLNSEEDAYSPFVSPLLYKDFSNLP
                     PAYIGTAGLDPILDDGSEYAEKLKSAGIDVIYRNYPNLIHGYVNMTAIPSCKDAILDA
                     CEELRLAFQK"
     gene            7808..>8194
                     /locus_tag="PLF66_24405"
     CDS             7808..>8194
                     /locus_tag="PLF66_24405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67252.1"
                     /translation="MFQFKKISTKFISLAVGALAIAIILTGGISYFTAKSSIISKLKS
                     TDLIQIASLKATKIDSRIQRAIETSNLIANDPTILRWFEEGETNYLLGELVRAKLNSS
                     ITSSEYTNAFAADRVTLNYWKNNSTTS"
BASE COUNT         2898 a         1295 c         1329 g         2672 t
ORIGIN      
        1 aactgcttct aaaaactcct tacttgaatt tactgtcttc aatgtgcctt tttcttttaa
       61 tgaaattgcg tcgaatgaat tgagtatctt ttctcctgtt atcaatggca aaccaaaaaa
      121 agcaggttct agaacattat gcactttatt gtgcatagcc ccacctatat aagcaatttt
      181 accgtaaaaa tacgcaaagg ctagtattcc taaaacatca aaaaggataa ttctatcttt
      241 acaagaattt tttgcctcac tgtaaaggga aactcctagg tttcttttct ttaaagatgt
      301 aagaatttcc tcaattctag atgagtttat atggtgagga aaaatccaga tggtaaaatt
      361 gaacgaaagc aaattctcaa tatttggcaa taggatagag tcacagtctt tataagtgga
      421 agctaaaata aaaataggtt ggctttgttt ttgagcaaac tttacaaatt cttcatttgg
      481 cttcttactt tgtattttat gataaacgga atcgaatcta gagtctccaa aggctttaat
      541 ccttactttt gtatcactta actcttgaaa ggattctacc atgctttcat ctactggaga
      601 gattgcatac aatctagaaa atactgcccg agttaaacct ttagcaaaaa aggaggatct
      661 ctttgatttt ggatctaaaa cagcgcaggc aagatagacc ttacagttgt gtcgatttgc
      721 tgctaaaacc aaatgtggcc aagtatccca agccattaaa actaatcttt ccggagaaaa
      781 ttttttaaat acaaagtcat aagaaaaata gaagtcgagc ggaagccgaa atgttaaatt
      841 ggtattctca gtatcataat ttttttccaa aacactatcc gaaaaaactg attggagtag
      901 gaaagtatct ttttcattct ctcgtatcac ttttgcaaga gccttacatt ggtccaattc
      961 tcctacggaa gatgcatgta accaaatcac tctttgattc ggctccaaaa aaaaatcctg
     1021 aatcttcttt ttatctaagt gtcttttgtt caaaaaggat tttgcttttt tattcagaag
     1081 gggaaggata agggtaaaag gaaagagtaa gcttgtaaga actgtataaa aaagaagcat
     1141 taattcaatt tcaaagaagg ttaaaacttt gtcaccaagt aaattcttta ctcttcctgt
     1201 ttatgtattt ataaaaaatc cgaaattaaa tgcatgagat caatgccaat tctatccttt
     1261 tcctttctat ttcttctaaa ttctttaact atacatacac aagaggctga agattataca
     1321 aaaaaaatag aaatgatctg gggatgggaa gaagagacca aatcaatcaa caaaaaagaa
     1381 ataaatattg aaaaccctgt gccagttttt ctctcccaat tccaaccaca aaaacaagaa
     1441 tttccaatca atccaacaat ggaaaatatt ataaaaggaa aggcaggaac aaaaattaca
     1501 attccagcac attcaatagc attgccatct gaatttagaa aaggtgatat actaacgatt
     1561 cagttgattg aaatttataa cgatttagat tttattacat caggtgttag tcttttctat
     1621 ttcaaccctg aaccaaatat atttgagtct ggtgggatgt ttaaactaac ggcgacttat
     1681 tatgataagc ctcttcaatt aaaaagaggg gctagattaa aagtagaaat tccttctcat
     1741 ataaaaacag ataaaaagat gaatatgtat cgattagatg aaagaaatgg ctggatcgat
     1801 aaaggcagtg tatccgttga aaagtcttct gaaaaaatag agaatcctgc ttccgaggga
     1861 cctgataaaa aaaaggatgg aagttctgaa agcggggtag aatacattcc atcatatata
     1921 tttcgggcaa tggatgaatt caaatggtgg aatgctgatt atcctaatcc aaatagaact
     1981 tgtatagaag gaacaatcca aacctttgaa gcaaatcctc cgtatagtat aacgtctcta
     2041 gggattgatt ttaaaaatgc gacaactcgc tatgctgacg gtaagaaatt tcagctgaat
     2101 gtaatcaaag caaagaatgt aaaactcatt gcagtcgatg agagaggaaa cataggactt
     2161 atatcagaaa tcaacacagg aaatttaaat gcatttatca aagaaggcag caaagaaaaa
     2221 tgcacaaatg ttggaaccat tgaaatcaaa aaagttgata gagagatttt aaaagataga
     2281 ggaaaacttt tgaatcattt aggtttgaaa gatattgtga aataatatca gcgcagaaaa
     2341 gcttgcgata ttctattaaa attctgtttt caattttgaa aaattcttac ttaaaaaata
     2401 agcacaaaaa ccgccaggga gattttcctt gctttcttct ggtataaata attcttttga
     2461 aaaataaaaa ccatcctctt taataaaaga ttttaaaatt cgaattaacg taagaggcga
     2521 aaaacctgta gaatgacaag ataatattac aaattcaggt ttgccatcag atagtcttat
     2581 catctcatca attaatggaa ttaaatcatc ttctattttc caaacttctc ctttggcacc
     2641 tcttccataa gtgggcgggt ctaaaataaa ccctttgtaa atacgatttc tttttacttc
     2701 tctttttata aattttaaaa catcatcttc aatccaacgg atctttcgat cagttaaatt
     2761 agaaagttct gcattttctc ttgcccaagc aatcatacct ttcgaagaat ctacatgaca
     2821 tacgggataa ccagcattag ccgctgaaag agtagacatt ccagaatagg caaatagatt
     2881 taaaacttcg aaattatttt gaatccctcc taatttttga agcaatgtcc aattgaattc
     2941 ttgttctgga aaaattccta tatgaccaaa aggagttaat ttgattttag cggatagatt
     3001 tgctatctca atagaaaaac tttctggaat cttcttgtaa aatttccaat gaccgcttcc
     3061 tgtatcattc tttaaataat gagcatcggg gttcttccat agactcggat cttttttttt
     3121 gtaaggagaa ccaagagcag gacgaatgat tttatatccg cctattattt ctaatttttc
     3181 ataatctcct gagtcaatga gtctatagct ttgtttcatt gtgtcatagt ctagtatggt
     3241 ttatcatctg caaattgttt ttactcacta tttctacaaa gcctaatatt tactttctaa
     3301 aatactttgt ctaatttttc tcttctttat gtttctatag ataaaatgaa ttaaataaat
     3361 tttttctttt caatttggac gaattttaaa tattcgcatc atgtatttat acgagttaga
     3421 gcaaaatcta aacaaagtca ttaccctttc aggttgggtt cacggaaaga gaggaaacaa
     3481 taaaattcaa tttctccaaa ttcgaaattc cggaagaatc atacaagtag tttgcgaaaa
     3541 agaaaaacta ggtgaagatg tttttgaaaa aataaaagca ttgcatcagg aaacttctat
     3601 agaactaaaa ggccttcttg tcgtttcaga aaaatctgaa ttaggatacg aaattatatt
     3661 agaatcctat aatgtagtag gagatgcaat tgattatcca attacaccga aagaacacgg
     3721 tcctgatttt ttacacaacc atagacatct ttggctcaga tctaaaaaac agctctctat
     3781 tattcaaatt agaaatgagc tttcctttca aattagaaag tatttttacg atagacacta
     3841 tatattaata gatacaccaa tacttactgg ttcagtaggg gagagtgctg gcactttatt
     3901 tgaaacagaa tattttgatg aaggaaaagc ttatttagct caaacaggac aactttattt
     3961 agaaacagca atttttgccc atagtcatgt ctattgcttt ggaccaacat ttagagcaga
     4021 gaaaagtaag actagaaggc atctaacaga attttggatg ttagaagcag aaaatgcaaa
     4081 ttatcgaaat gaagataata taaatctaca agatgacttc atacgaacta tcgtcaagaa
     4141 tacggttaat gcttgtttac ctcatttgaa aacactagaa agaaattcag agacaattct
     4201 aaattatgta gagaaaccat ttcaaagaat tacttattct aaagccattg aggttttaaa
     4261 ggaaaaaggg gaagggattg aatggggtga agacattaat gcagaaagag aaaatatttt
     4321 aaccactcat tttaatggtc cggtatttgt aatgaattat ccaagagcaa ttaaggcttt
     4381 ttacatgaaa caaaacccag aggatgaaag aactgttttg tgtgcagatc taattgcacc
     4441 tgaaggagtt ggagagatta ttgggggttc cgaaagagag gaaaactatg ataagattgt
     4501 ggagcgctta aaagaagaaa aactcccagt agaaaattac tcttggtatt tagatttaag
     4561 aaaatatgga tcagtcccac attctggatt tggattagga ttagagagat tagtcgcttg
     4621 gatttgtggt ctaccgcatg tgagagaatg tattcctttt ccaagactaa tgggaagatt
     4681 acacccctaa gaaaacaaaa aactataaaa aattataacc tcttttttgg atttgcccga
     4741 aatttattaa agaggttatt atgatacaat ctaatactta ttcatctact aatctagaaa
     4801 gagaagcttg ggagagcttt gaagttggct cctttgagga tgttatcaat gctgttgagc
     4861 aaaacccttc gagtgcttta ctaaatcatc tagcaatttt agcaaaatat gaattaggca
     4921 atagatcaaa tgatgaccta aaaaagtttt cagcaaacgg tttatcagta tttactcctt
     4981 tagttgcagc ttataatgca ttttatagcg gaaaccgctc actagcttta cagaagataa
     5041 aagaatactt agaaaaaaat aatcctccga tttgtttaat tataattaat tttgcggtga
     5101 aagttgcatt cagtgcagaa gcctatgaag attgtctgaa aattatttat ttatctgaaa
     5161 agaaaacagg caatccaaat gaatttgtaa aagaaagaat agaatgttac ttccaattaa
     5221 aaaagcacaa tgaactcata caatatttta agtctgttta taaattacta gattcaaatt
     5281 atgatattta tttaaaagca ggtctttctt taaatgctct aggaaagtat aaagaagcag
     5341 aatatctttt tggaaagatt cctggtaaaa gaaatctacc aacttatgat gaaaagaagc
     5401 aggaattcaa accaatcata aataaaatat ctgaatttga gaaaaaagaa gttctttcta
     5461 actctgaatt aaaagattta ggatttgcgt atctatttaa tgcagagtat aaaaaagcag
     5521 aagaagtttt taagaaagtt gccgtcgcaa tacaataatc actttacatt tctttaaatt
     5581 tttcttagtt tcttttgttg ctagaatctt agtagcttct tttgtaggaa ttgtataacc
     5641 agttttgttt ttccctataa cgttagttgc taccactagg tagggatttt ctccaaccct
     5701 ttgatgtttc atcgctttta taggatcact catataaatt acaaatccat tatcgatagc
     5761 aaagttttta tttacaaggc tcatggaata gatttcaaga cccttatcag taaaaattct
     5821 aggaaataac gcaggatttg catctaaatg tctagcatca atgatgagtc ctgtgtatgc
     5881 ttctggaaca ggaatttcca taaattctgg aaatttttct gtatcgtaat ttatcatgag
     5941 atagttcata agacctttag agcctagtag ttttgtaagt gactcaactc gaacttcatc
     6001 ttgaatgtat tttactttga tttcactgga ttcatttaag aaaaactcat tgaatctttc
     6061 tctaaatttt tcatctttat ttactttttc tataatagta tattcttcat ttaaacgaat
     6121 tgattcaatg gatcgaacaa aatggatttt ggttttttct tttgctgtaa gtagactttt
     6181 atttcttgcc tctgaaatat tataagctgt gttctccttt ccatattcag gatcatctac
     6241 atcaataata accctcggga gcttttcaga agcatttaaa gagatctgca tcgttgacca
     6301 attcacaact gtatcaaaaa aatctttgat aatcagttga gatttttcat ttgcataaga
     6361 tgggataatt aataataaaa ggattaaagc taatattcgt agtatcatta tttttacagt
     6421 cggctgaaat ctatttacta tcaatcatta tttaagattc ctgatccctc ttcttcttat
     6481 aatggttcat aagaatatat tttgaacgaa aaaatatttc ctttttttac tctttttaaa
     6541 tcgaaaatgc ttctttttag ttctttttat ttctgaaaag ccaatcgtaa ttcttcacaa
     6601 gcatctaaaa tggcatcttt gcaagatgga atcgcagtca tgttcacata cccatgaatt
     6661 aagtttggat aatttctata aataacatca atgccagcag attttaattt ttcagcatac
     6721 tcggatccat catctaaaat gggatcaaga ccagcagttc caatataagc aggtggcaaa
     6781 ttggaaaaat ctttatacaa taatggagaa acaaatggag aataggcatc ttcctctgaa
     6841 tttaaataat tctcaataaa ccaatcaatt tgcttgcctg tcaggaaaaa gccttcacca
     6901 taacgatatc ttgagtccga cttacgagag gcatctgttc caggataaat caaaaattga
     6961 aaaaatggtt tatactctgt attttttagt tcatgagata gaacagtagc tagggttcca
     7021 cctgcgctat ctccaccggt tgcaatgcga gagcgatctc cttccacaaa ttcaatgtga
     7081 tcacagaccc atttataagc tgtaattgta tcatatacag gaatcggaaa tttatattct
     7141 ggagccaaac gatatccaac agagactaca atacaatggg cattttcaca gataatttca
     7201 caaattttct catgagaatc aacactaccc ataacataac cgccaccatg gttataaact
     7261 aaaacaggaa gtttttcctt ggttctaatt ctaggtttga atatacgaag aggaattttt
     7321 cccttcggat tttctactgc aatttgaata ttttcaatct caggtttgtg attgattggt
     7381 gcttctataa gaccagataa agcttctaat acttgtcgag catccttagg tgcaagtgtt
     7441 gcaaagcttg ggaagatact taaaacagtg gttaccaatt gtaagccagg atctaaagtt
     7501 ttattatcaa tctgaattgg cttttttccc gataataaat aaagagtgct tttgggaagt
     7561 gagaataaat gttttaaatt ctttgcaatg gaaactttca tttcgttctg tatttatact
     7621 tgtcaatatg gtatgtataa atttaaaagc aagtagtagt ttagaattga tgcttggaaa
     7681 aattaaaaaa ttacagagcg agttttgatt ctaggtatat taggaatttc taatgaaaga
     7741 gaaaaccatt ctgtcaattt cacgaacatc ctttatatac aaaagttgaa gtaaggtaaa
     7801 tagataaatg tttcaattta aaaaaattag cacaaagttt atatcccttg ctgttggcgc
     7861 acttgcgatt gctattattt tgacaggtgg tatcagttat tttactgcaa aatcatctat
     7921 tatttccaag ctaaaatcga cagatttaat tcagattgca tctttaaaag caactaagat
     7981 tgattctaga atccaacgtg cgatagaaac ttcaaattta attgcaaatg atcctacaat
     8041 ccttagatgg tttgaagaag gagaaaccaa ttatcttttg ggtgaattag ttcgagcaaa
     8101 actaaatagt tccatcacct cttcggaata cacaaatgca tttgctgcag acagagtaac
     8161 acttaattat tggaaaaaca attccacaac tagc
//
LOCUS       DAOQOS010000190         7966 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_5048_length_7966_cov_6.181267, whole genome shotgun sequence.
ACCESSION   DAOQOS010000190 DAOQOS010000000
VERSION     DAOQOS010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 7966)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7966)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7966
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5048_length_7966_cov_6.181267"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            46..1074
                     /locus_tag="PLF66_24410"
     CDS             46..1074
                     /locus_tag="PLF66_24410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67253.1"
                     /translation="MEEENNIKEELEHLTSLYLFEEINSEEKNRLFSILNSSPIYKAE
                     FSELKALNDQLVKEKDLTQLEKNPSNKIFIFKKKFYIPLTIAAVLLFGLFLQKDFTKE
                     KIEIGILEKKEYGKCNLKTKVDTVSYQSDKYSICEVVFKTKNLYQILLYPESNLTATL
                     DNSHLTINQFSGIMILDSHQISKEETLQIKLNSTYIHFYGTKIKLESHKEKKISVLEG
                     KIGIQNDPTNKETIVSRGEETNWNQITPVVPISKNDLKTMTTISEILKKQKASSNQID
                     PVLVEEILHKKESVFLSKIAGKKVLLKSGKSIVAISIFQKDTTYLIFTEKEVIQLESV
                     EVESIEFE"
     gene            1061..1894
                     /locus_tag="PLF66_24415"
     CDS             1061..1894
                     /locus_tag="PLF66_24415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67254.1"
                     /translation="MNSNKLIPLLLYFSFLVFLQGDDVELKNGKTYSNVKTNLQKDKL
                     KITFEDGKSIQVPISSIKKIKPQEVKWNPKIETDTDTNVKTIETKEIETKSEDNTDLP
                     ITNDSKKNTNEDFVLESFIPFWSGLNRTNLWYLGISLSALELYTFYNFTQFPRQPKEP
                     IPDFAFLFYLNEIQRDRGIRPEERQDIRTYLFLNYYNNNHTILPSGEIVSNEHYLLER
                     RQAFRGFLFAILLDVGITYLFSGKDPLATSNSNRNFYFSISPNLSTKSHGFDFGFHFY
                     Y"
     gene            complement(2025..2588)
                     /locus_tag="PLF66_24420"
     CDS             complement(2025..2588)
                     /locus_tag="PLF66_24420"
                     /inference="COORDINATES: protein motif:HMM:NF023273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HMZ67255.1"
                     /translation="MKKIILVILSIFIFQVCKKNTNEKVFLEEAKETALQAKSELGKN
                     LSNAIKSKGTAEAIVFCNQKAIPLTIEMSEKAGMLLKRVSDKPRNIVNTANEEEKNII
                     DEFKTQLSKGEKLKPVLKETNVEKIAYLPIETNAMCLQCHGSLEKDISKEVQQKLKEL
                     YPNDKAVGYSENQMRGMWVVSKKLESN"
     gene            complement(2770..3441)
                     /locus_tag="PLF66_24425"
     CDS             complement(2770..3441)
                     /locus_tag="PLF66_24425"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HMZ67256.1"
                     /translation="MKNFLLIFIMLWILLKCSHHHHHGDANEHMNQTSFEDLTSSFEN
                     PERDKWQKPKEVLAFLEKESSKKSLKGLSVADLGAGTGYFSIRLLDAGAKVIALDADE
                     RFLNYIQKKAESNPRKTSLSLRKIPYTSAELKKEEVDILLSVNVYHHIENRVSYFSEI
                     KKSLKKDGFICIIDFKEGKLPVHSPPESMRISKEQTIDELQKAGFKVSVEEKTLDYQY
                     IYVAK"
     gene            3887..4162
                     /locus_tag="PLF66_24430"
     CDS             3887..4162
                     /locus_tag="PLF66_24430"
                     /inference="COORDINATES: protein motif:HMM:NF021558.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2281 domain-containing protein"
                     /protein_id="HMZ67257.1"
                     /translation="METLFRLSDLFLPLSEEKQQEVLDFVEFLYAKQSLSKSNQPLTV
                     PENDVNIIPSEKLKNLLRERLAQHRANPQAGKNWNELKVELLDKYRK"
     gene            4162..4434
                     /locus_tag="PLF66_24435"
     CDS             4162..4434
                     /locus_tag="PLF66_24435"
                     /inference="COORDINATES: protein motif:HMM:NF016876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HMZ67258.1"
                     /translation="MYSIIVSPPAEIDIKEIQEYYDNLADGLGERFVLRLSKVFEFLE
                     RHPKLYPVIELEIRKVIMQSFPYAIFYSINENKIRYSCCYSHKSKS"
     gene            complement(4913..5164)
                     /locus_tag="PLF66_24440"
                     /pseudo
     CDS             complement(4913..5164)
                     /locus_tag="PLF66_24440"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
     gene            complement(5210..5401)
                     /locus_tag="PLF66_24445"
     CDS             complement(5210..5401)
                     /locus_tag="PLF66_24445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67259.1"
                     /translation="MFVALELLAQSGFGDKVTLKNGKVLENVRTIVTTDSLVVTGTDG
                     ITTVYNKREVAEVKKSDKK"
     gene            complement(5923..7407)
                     /locus_tag="PLF66_24450"
     CDS             complement(5923..7407)
                     /locus_tag="PLF66_24450"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HMZ67260.1"
                     /translation="MSTFLIGFIFLLTLGFLYILYSYARGLKDSPREIWILFTAKLLE
                     YAAYGGSNMAFVLYLSKDCGLNDVSAGTYIGIWSTVMTCFSMLGGSIVDAIGVKRTLI
                     VGMLAMLLGRLVMPFSNHLLFVTVFAFIPVAIGNAILVPVLSVGIKKFTTKESTALGF
                     GLFYTLMNVGFAIGGYVFDKTREVYGEHSQIDLGFIYISTYQMIFLISFLLTLPSFLF
                     ILLMRKDIRVNLNQEKTFKSKNVLNSIWFEIKKSSKDTLAIMKTVFVEKAFWTYLFML
                     GILVFVKLTFYHFNYTFPKYGIRVLGEGSKVGAIYGILNPVLIIFLVPLISHLTKKIK
                     SYTMLLLGTSLSTVSIFIAALPLSFFEKLNSSFYSKLIFEDWLSLPVERQNPIILGIV
                     LFIILFTIGEAIWSPRLMQFSAEFAPEGKEGTYIALSYLPYFLAKMIAGPMSGFLIAR
                     YVPEGASEYPEHFMVWVWIGSVTLLSPVGLFVFRKMFERNRKNE"
     gene            complement(7404..>7966)
                     /locus_tag="PLF66_24455"
     CDS             complement(7404..>7966)
                     /locus_tag="PLF66_24455"
                     /inference="COORDINATES: protein motif:HMM:NF012345.2"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HMZ67261.1"
                     /translation="SSSSLKDYLDKFDANDKLIGVISLGSYANVGDNLDWVKQYGKDL
                     KENIIEKGVPFFGICFSHQLLAWIYGANVSFIENRESLPEKKYNEFREITITHPKIKK
                     IYNHKETFISRAKHEQEVKDLPIELELGGFSSHCKIEILVHKYLPAFSVQSHPEEFHE
                     TGEGILLVKNIIVFFYTWLGNWERIS"
BASE COUNT         2710 a         1425 c         1166 g         2665 t
ORIGIN      
        1 taaagaagaa gcgattaaga agaacttaga actttagggg taaatgtgga agaagaaaat
       61 aacataaaag aggagttaga gcatttaacg tctctctatc tttttgaaga aataaactca
      121 gaagagaaaa atcgtctctt ttcaatttta aattcttctc caatctacaa agcggaattt
      181 tcagaactaa aagcactcaa cgatcaattg gtaaaagaaa aagatttaac tcaattggaa
      241 aaaaatccat ccaataaaat atttattttt aagaagaagt tctatattcc tcttacaatc
      301 gccgctgtac ttttatttgg tctttttctt caaaaagatt ttacaaaaga aaaaattgaa
      361 attggaattt tagaaaaaaa agaatacgga aaatgcaatc ttaaaaccaa agtggatacg
      421 gtatcttatc aaagtgataa gtattctatt tgtgaagtag tatttaaaac aaaaaactta
      481 taccaaattc tactctatcc tgaatcaaat ctaacagcta ccctagacaa ttctcattta
      541 acaatcaatc agttttctgg aattatgatt ttagattccc atcaaatctc aaaagaggaa
      601 actcttcaaa tcaaactcaa ttcaacctac attcattttt acggaacaaa aattaaacta
      661 gaaagtcata aagaaaaaaa aattagtgtt ttagaaggaa aaataggtat tcaaaatgat
      721 cctacaaata aagaaacaat cgtttcgaga ggtgaggaaa caaactggaa tcaaatcaca
      781 cctgtagtcc ctatttctaa aaatgatctc aaaactatga caacaatttc ggagatttta
      841 aaaaaacaaa aggcatcttc aaatcaaata gatccagttt tagtcgaaga aattctgcat
      901 aaaaaagaaa gtgtttttct ttctaaaata gcagggaaaa aagtcttact aaaatcaggt
      961 aaatcaatag tcgctatctc tatttttcaa aaagacacaa cttacctgat ctttactgaa
     1021 aaagaagtaa tacaattaga atcggtagaa gtggaaagca ttgaattcga ataaattaat
     1081 tcctctactt ttgtattttt cttttcttgt ttttttacaa ggagatgatg ttgaactaaa
     1141 aaatgggaaa acatattcca atgtaaaaac caatctacaa aaggataaat taaaaattac
     1201 attcgaagat ggaaaatcaa ttcaagttcc aatctcatct atcaaaaaaa taaaacctca
     1261 agaagtaaaa tggaatccaa agattgaaac agatacggat acaaatgtaa aaacaattga
     1321 aacaaaagag atagaaacaa aatctgagga taatactgat ttacctatta caaatgattc
     1381 taaaaaaaat acgaatgagg attttgtatt agaatcattt ataccatttt ggtctggatt
     1441 aaacaggacc aatctatggt atttaggaat ttctctctct gcacttgagc tttatacctt
     1501 ttacaatttt acacaatttc caagacaacc gaaagaacct atacctgatt ttgcctttct
     1561 gttttatcta aatgaaatcc aaagagacag aggaatcaga ccagaagaaa ggcaagacat
     1621 tcgaacctac ctttttttaa attactacaa caataatcat acaatcttac catccggaga
     1681 aatagtatcc aatgaacatt acctactaga aagaaggcag gcatttagag gctttttatt
     1741 tgcgattcta ttggatgtag ggattaccta tctgtttagc ggaaaagacc cattagccac
     1801 atccaactct aatcgaaatt tttatttttc tatttctcct aatctttcaa ctaaaagtca
     1861 tggatttgat tttggctttc atttctatta ctaattcact caaactaaat ttttactcag
     1921 atgaagagga ttgattccag tttttggatt cagaaagatc agagatgtat caatgattta
     1981 ctccaaatac cattcaaatg gaatcttttt gagaaataac tttattaatt cgattctaat
     2041 ttcttagaaa caacccacat ccctctcatt tgattttcac tgtatcctac tgctttatca
     2101 tttggataaa gttcttttag tttttgctgc acttcttttg aaatgtcttt ctctaaactg
     2161 ccatggcatt gtaggcacat agcatttgtt tcaattggta aataagcaat tttttcaacg
     2221 ttagtctctt tcaaaacggg ttttaatttt tctccctttg aaagttgtgt tttaaattca
     2281 tcaatgatat ttttttcttc ttcatttgct gtatttacta tatttcttgg tttatcagat
     2341 actcttttta atagcattcc agctttttca gacatttcta ttgtgagtgg aattgctttt
     2401 tgattacaaa atacaattgc ttctgcagtt cctttgctct tgattgcatt agataagttt
     2461 tttccaagtt ctgattttgc ttgcagagca gtttcctttg cttcctccaa aaatactttc
     2521 tcatttgtat tttttttaca aacttgaaat atgaaaatcg atagaattac taatattatt
     2581 tttttcattt tgttccttcc tctctagtgt ttttctaatc tttaactcat catatattat
     2641 cattgcttca taaaagcaag attttcacca cgaagaaaaa ttgtaaattt cctagtaaat
     2701 tttgactttt gcgatcttct tgctcttcgt ggttagattt tttaagcttt tactcagtcc
     2761 ctaatatatc tattttgcaa catagatata ttgatagtct agtgtttttt cttccacaga
     2821 aactttgaat ccagcctttt gcagttcgtc tattgtttgt tcttttgaaa ttcgcatcga
     2881 ttcaggaggg ctatgaacag gaagctttcc ttccttaaaa tcaataatac aaataaatcc
     2941 atcttttttt aaacttttct taatttccga aaaatacgaa actctatttt caatatgatg
     3001 gtatacgtta accgaaagaa gtatatctac ttcttctttt tttaattccg cacttgtata
     3061 tgggattttt ctaagactca aagaagtttt tctaggattc gattctgctt ttttctgaat
     3121 gtaatttaga aaacgctcgt cggcatccaa agcaatgact ttagctcctg catctagaag
     3181 tcggatagaa aaatatcctg tgccagcgcc tagatcggca acactcaaac ctttcaaact
     3241 tttcttagag ctttcttttt ctagaaatgc taaaacttcc tttggctttt gccatttatc
     3301 acgctctgga ttttcaaaag aagaagttaa atcttcgaaa gatgtctgat tcatgtgctc
     3361 attggcatca ccatgatgat ggtgatgaga gcatttcagt aaaatccaga gcattataaa
     3421 aattaaaaga aaatttttca taaaaacaaa tctcctaaat taatttataa aataaactca
     3481 taattattct ttttaaaatt cttgaatctt ttttgaatta cataaaaatc tttgcggccc
     3541 tgagtgaaac tcttattcac taaacactaa atctaactta aagttagttt ccaagataaa
     3601 tttaggataa gtttaataga tctttactcc taagtttaag ggatgtttgg tgatacgttt
     3661 gtattactct atatagaatt ctttttgctt tttaaatgaa agctttcttg gtttactttc
     3721 tagaaatttg cattccattt ttttcacaac cgtatcccat attatccagc aaattaaaag
     3781 ttgaaaggaa aaatggattc tgagtctttt ataacttggt gtcttgaact agagaaggaa
     3841 tttttttcag ctgacaaatg catgaattca atgctccaat ataaggatgg aaactttatt
     3901 tcgattgtcg gatttatttt taccactctc agaagaaaaa caacaagaag tgcttgattt
     3961 tgttgagttc ctatatgcaa aacagtcttt gtctaaatcg aatcagccgc tcactgttcc
     4021 agaaaatgat gtaaatataa ttccttctga aaagttaaaa aatcttctca gagaacgtct
     4081 cgctcagcat agagcgaatc cgcaagctgg aaaaaattgg aatgagttaa aagtagaact
     4141 attagacaaa tataggaagt aatgtattca attatcgtat ctccgcctgc tgaaattgat
     4201 ataaaagaaa ttcaagaata ttatgataac cttgccgatg gattagggga aagattcgtt
     4261 ttgagattaa gcaaagtatt tgaattttta gaacgtcatc cgaaattgta tcctgtcatc
     4321 gagttagaaa ttagaaaagt tattatgcag agttttccct atgcaatctt ttattctatt
     4381 aatgaaaata aaattagata ttcttgctgt tattcacaca agtcgaaatc ctgaatattg
     4441 gcgaactaga attacaattg aataggcttt atatactaaa tgacaaaagt aattttccag
     4501 aagaattttg atccactagt atttggaata ccgcacataa agacgtgcgg gaacactaag
     4561 aagtgtttag accaaaccca cttttttatt tttagaatgg gacttttctt ttgacgttta
     4621 atataaatct tgaattttag aatttgatgc tttgattttt gcatagttcc attaattccc
     4681 acgagatatt ttcattccgc ttcgtagaag attccatccc tccactaata ctgcaaacgt
     4741 aaggattttt aagcacatag cttggcttat ggtgaacgtc aagcgatggg gggaatgcac
     4801 gaagagtcac gaagggaata aataataaac cttctctttt cttcgtgttc tctgtgctct
     4861 tcgtggttaa ttattcttca tttctctaac tttgacagct cccaccttcc actcactcca
     4921 ttacatcctt ccaccttact tgctctaacg gatagtcttt cccacaatct ttaaaataac
     4981 tgggattgtt tgagaaaaag aatgagccca ttaccgcttc ccattgtccc tgggttactc
     5041 cgtatttgcc catataaaat gattttgtga ttttcacttt atgcgctggc ttttctgatt
     5101 cgccgcattg actgtcattc ggagaacagc tcatcataaa ttctcccgca gaaatttcta
     5161 caaactccat accgattgag tttttgattg ttttacttgt aaacttactc tatttcttat
     5221 cactcttctt aacttccgct acttctcttt tattatacac agtcgtaatc ccatcggttc
     5281 cggttacaac gagtgaatcc gtagtgacta ttgttcttac attctctagg acttttccgt
     5341 tcttgagagt gactttatcc ccgaatccag attgggcgag taattccagt gctacaaaca
     5401 ctgcacataa cgccgttatg ccgatatttt ttttgattca ttgtgttttc ctttaattta
     5461 gtgtacagaa aaaaatctgg cagtaagcat atgttttgac tcaaagagag aaatattgag
     5521 tgtatcgaac atgaccttgt tatctatcta ctatcgtaat ttcttaaact tgtttctggt
     5581 gtgcaagata tttttgtcat tttatgccgg gaaaacagca ctgagttttg acgcaaaggc
     5641 gctaaggggt ttatttttca ttccaatcta tttcaatacc agcgcgaatt tcaaatcata
     5701 catccattct gaaaaaaaac acaattacaa tcacaaaacc catcgttgtt tatccgtgtt
     5761 catccgtggt aatcttttaa aatcttttct tattgaatcg aatttttcaa tctgcaaaca
     5821 taacggagcc agatcgtgat ctgcctctac aatttatata tataatcata accaaaacca
     5881 caatcccatc ggtgcctatc ggtgttcatc cgtggttaac tcttattcat ttttcctatt
     5941 cctttcaaac atctttctaa aaacaaaaag ccccactgga gataacaacg ttacgctacc
     6001 aatccaaacc caaaccataa aatgttctgg gtattcactc gcaccttcag gaacatagcg
     6061 tgcgattaaa aaccccgaca tgggaccagc aatcatcttt gctagaaaat aaggaagata
     6121 agagagtgca atgtaggttc cttcttttcc ttctggtgca aattcagcag aaaattgcat
     6181 aagcctaggt gaccagattg cttctccaat tgtaaaaaga ataataaaga gaacaattcc
     6241 gaggataatc ggattttgcc tctcaactgg gagagaaagc cagtcttcaa aaataagttt
     6301 ggaataaaaa ctagagttca atttttcgaa aaaagagagt ggcaatgctg caataaaaat
     6361 cgaaactgta gaaagactag ttcctaatag cagcatagta tacgatttta ttttttttgt
     6421 aagatgactg atgagcggaa ctaaaaaaat aatcaatact ggatttagaa ttccgtatat
     6481 cgcaccaacc ttactcccct cccccaaaac tcgtatccca tacttaggaa atgtataatt
     6541 aaaatgataa aaagtcaatt tcacaaagac taaaatccca agcataaaaa gataagtcca
     6601 aaaagctttt tcaacaaaga cagttttcat aatcgcaagt gtatctttgc tagacttttt
     6661 aatctcaaac caaatcgaat tcaaaacatt tttagattta aaagtctttt cctgatttaa
     6721 attgactcga atatcttttc gcatcaataa aataaataaa aaggaaggaa gcgttagtaa
     6781 aaaactaatg agaaaaatca tctggtaggt agatatataa atgaaaccta aatcaatttg
     6841 agaatgttct ccataaacct ctcttgtctt atcaaacaca taaccaccga tagcaaaacc
     6901 aacattcatc agagtgtaga atagaccaaa accgagtgct gtgctttctt ttgttgtaaa
     6961 ttttttaata cccacagata aaacgggaac caaaatcgca tttccgatcg ctactggtat
     7021 aaacgcaaac acagttacaa agagaagatg attcgagaaa ggcattacaa gtctaccaag
     7081 aagcatagca agcataccaa cgatgagtgt tcttttaaca cctatcgcat ctacaatgga
     7141 tccaccaagc atggaaaaac aagtcataac tgtagaccaa atcccaatat aagtcccagc
     7201 gctaacatca ttcaatccac aatcttttga aagataaagc acaaaagcca tattggatcc
     7261 accgtaggct gcatactcca aaagttttgc tgtaaatagt atccaaatct ctctgggaga
     7321 atcttttaaa cctcgtgcat aactgtatag aatgtataag aatccaagcg ttagtagaaa
     7381 gataaatcca atcaagaaag tactcaagag attctctccc aatttccaag ccaggtataa
     7441 aagaaaacta tgatattttt gactaataaa ataccttctc ctgtttcatg aaattcttcc
     7501 ggatgagact gaacggaaaa tgcaggtaaa tatttgtgaa ctaatatttc gattttgcaa
     7561 tgggaactaa atccaccaag ttctaattca attggcaaat cctttacttc ctgttcatgc
     7621 ttagcacgag aaataaatgt ttctttgtga ttgtatattt tcttaatttt gggatgcgtg
     7681 attgtaattt ctctaaattc attgtatttt ttttcaggta ggctctctct attttctata
     7741 aagctaacat ttgctccata gatccaagca agcagttgat gagaaaaaca aattccaaaa
     7801 aagggaacac ctttctctat aatattttct tttagatctt tcccgtattg ttttacccaa
     7861 tctaaattat ctcctacatt tgcatagctt cctagggaga taacaccaat tagcttatca
     7921 ttcgcgtcga atttatctaa gtaatctttt aaactactgc tggaat
//
LOCUS       DAOQOS010000191         7959 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_5057_length_7959_cov_6.779605, whole genome shotgun sequence.
ACCESSION   DAOQOS010000191 DAOQOS010000000
VERSION     DAOQOS010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 7959)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7959)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7959
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5057_length_7959_cov_6.779605"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1082
                     /locus_tag="PLF66_24460"
     CDS             <1..1082
                     /locus_tag="PLF66_24460"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HMZ67262.1"
                     /translation="FGIPPVYIYGSIAYILLLPFAIVLNGNGKMKLARIHLFTLSIAY
                     LLLLTFMQGNQIKGHIFFLAEAFVVFFIFPAKEKKLMFLFVVFLTGLYIAFDIFHDQL
                     TPIVIQTPDKIKIANLITDISFAFILIGFAFYIHITFQRTELFMQLEHDKSEKLLQNI
                     LPISIIKKLRENPDTIAERFEDCTVLFSDIVGFTQMSKTMPAVSIVKLLNEIFSRFDD
                     LAEKYKLEKIKTIGDAYMVVGGLPEPDEDHAKKVASFALEMLEIINDYRKEKNIPIEV
                     RIGINSGAAVAGVIGKKKFIYDLWGDSVNTASRMESHGVPGHIQVSESTYRHLKDLFQ
                     FKDRGVLEVKGLGAVQSYLLTAKIG"
     gene            1147..1671
                     /locus_tag="PLF66_24465"
     CDS             1147..1671
                     /locus_tag="PLF66_24465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67263.1"
                     /translation="MNTILEELTEFGELLCLKPKIKIVSPSLKDKSFFFIKNGYLQKY
                     YSIKGKKHVFRLIESGDYCESFTVLQGEKNNYEYIETVSDTVIIKFDYQKSIIKINNS
                     LMLSNFFKDLLSNFLFFQEKRILNFLALSPKERYKEFIETNYQKIGKYPDHIIASYLG
                     ITKETFSRFRNRKS"
     gene            1750..2118
                     /locus_tag="PLF66_24470"
     CDS             1750..2118
                     /locus_tag="PLF66_24470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67264.1"
                     /translation="MENLYFGIAVSFLFIHELDAIKAREWKLFIFLKDMSEDKAYSVW
                     LALHFPLFLFPMMIYSKFFSPVYQYRIQFGFDIFLIAHLFLHILFRKNKSYYFTLIQS
                     NIYILLTALFAIADIVSLKG"
     gene            complement(2140..2922)
                     /locus_tag="PLF66_24475"
     CDS             complement(2140..2922)
                     /locus_tag="PLF66_24475"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HMZ67265.1"
                     /translation="MEHLTSVFLFFIGISIVFIPLERSFYQTISPIYRKEWLTDLLFY
                     LGQALVWNFVTVYILNQFFNWMEFSFVQSLQSYFSRQNFFLQIIEVILLSDLFIYWGH
                     RFQHKYEFLWRFHRVHHTAETVDYLAAFREHPLDNIYTRGLETLPAVLLGFDLNQIAG
                     YITFRGLWALFIHSNVNIRLGFLEILFGSPHLHHWHHDLETQGKCNYANLSPIMDLIF
                     GSYYSPKEAASKFGIQDKISHSYFRQMIDPILPQSFVNKRND"
     gene            complement(2932..3273)
                     /locus_tag="PLF66_24480"
     CDS             complement(2932..3273)
                     /locus_tag="PLF66_24480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67266.1"
                     /translation="MSKFETVFQKSFVKGFVFLLAVAGLTISVLYSQAESKKNKSEKK
                     IQHSSKSMVPSKKAKEKPEEKQAKQKEDTVIFEEDKKDQSKQEAKKEKKEVIDYPIMP
                     SSKAPALPNRN"
     gene            3538..4206
                     /locus_tag="PLF66_24485"
     CDS             3538..4206
                     /locus_tag="PLF66_24485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67267.1"
                     /translation="MAVAVQGGSSGGASGGDLVKKLLDATRSTNPVGNDKLSEAQLKV
                     AAVHIQAILNECAKEGVTDKGQIAYILGTVRRECVLGNKMIEVIDEAKANTNYSKEKM
                     PALGNGDIASGDGFRYRGRGYVQITGRGNYSKWSQKLGIDLVGDPDKACDPAIAAKIC
                     IVGMRDGLFAPAAGPLSKYINGNTRDFLNARAVVNGGELKRAPEKAKYIVAYAESYYK
                     ALTT"
     gene            4269..4910
                     /locus_tag="PLF66_24490"
     CDS             4269..4910
                     /locus_tag="PLF66_24490"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HMZ67268.1"
                     /translation="MLFKYIPFAILLTLINCYTSKDQVDKSYLIPLLKQTNEKQSILQ
                     VNIIGDSLSERSVGFGLQNKLGNNYKVNDFSVSGRSVYSWLFDISRPFNPQPNILIIE
                     LGTNDVMNDPSYDFIGYFNRLLTEIKLRSSARIILTAVPLTDDKGLQNKIKVNNNFIR
                     SLSANYTVTDLEAVFEANRSTIKLYPSYDPIHPNPVGYELIGEAYKKDILYVK"
     gene            5194..5739
                     /locus_tag="PLF66_24495"
     CDS             5194..5739
                     /locus_tag="PLF66_24495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002745461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HMZ67269.1"
                     /translation="MSKKFLPFLLLIFVFTNSAFANEAAILSTAKKLVGYIRFKKNDQ
                     AIALIDTKTFSKNLLLNHWEKIDPKDQADFEEAMKGYIKAKSFPIALQYFDKIDINYE
                     KVKMKGSEAELPASILYKGSEKITFSWVFTEKNGTYLVSDFLLAEGKLASEVNRVKQI
                     EPTYEKKGMKELIALIKKASK"
     gene            5742..>7959
                     /locus_tag="PLF66_24500"
     CDS             5742..>7959
                     /locus_tag="PLF66_24500"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HMZ67270.1"
                     /translation="MKNLISILCHIFLLRPFITLVALVLLIGLSIQQALKLSINSNQL
                     DLLPASMIEVIKAKDVVEMIGGNGFYIVALKIKDEYARDKKILSAVDAKKKGNLDLYK
                     SEMEQAEKIKQDHLDYYTKSEKKIKRYADKLTSELLKDKDIRYISYKYDTTFLKERLP
                     LYIKSSDMREARSRIKRKIDEEIEKLNPFYINLTGEEYTPTFDDILSKYQRLAKRDVF
                     DAYNISPDKGMLLLLVKPEGSFLDLNFTRNLEVKIKKVVADLKLEDKGIHVAYSGSYK
                     LNLDDYDSLVDALKPISMASLIGITILLFLFFRNPLFILILVISLLTGIALTFGVTGF
                     VIGRLNTVTTIMAAVLMGLGIDYGIQFLYRFREEFTLRDDLMTSVTETIFHTGIASLI
                     SALTTTSAFVVLMFSDFKGFSEFGLIACYGIILIALSMYFVTALQIAIIFRLFPSFKR
                     FFVWSQKESEESSFANRFFANPRLVLRVSIAIIIVISLFAPTVKFNYSGRDLLLENQE
                     SLLIYDEIGDRFDVSSDPQAIATDTLEKSEAVFDFFTPVPEHMQNSVDQVVSIWNIIP
                     PENQQKENLHILKQIKDDLKYIKPHMLTEEQQKHLPTVDKYLGVKDFSYYDVPFLFTN
                     QFTEVPTSKVKGHMLFIYPKVALWHGKDLIDFYDKVAQFEYPLISKRTLNAILYSTNI
                     DLDKNSDDHIIAKYTKEEEETILKIANSASKEELMAMNILPLTAQLIVDKRPFTSIE"
BASE COUNT         2784 a         1332 c         1326 g         2517 t
ORIGIN      
        1 ccttcggaat tcctcccgta tatatttatg gatctatcgc atatatatta ttacttccct
       61 ttgctatcgt tttaaatggc aacggcaaaa tgaaattagc ccgcatacat ttatttactc
      121 tttctattgc ttatttacta cttctcactt tcatgcaagg aaatcaaata aaaggacata
      181 tattttttct agcagaagcg tttgttgtct tttttatatt tcctgcaaaa gaaaaaaaat
      241 taatgtttct ctttgtagtc ttcctaactg gtctttatat tgcctttgat attttccatg
      301 atcaattgac acctatcgta attcaaactc cagataaaat caaaattgcg aacttaatca
      361 cagatatttc ctttgcgttt atactaattg gatttgcttt ctatattcat attacctttc
      421 agagaacaga attatttatg caattggagc atgacaaatc agaaaaacta ttacaaaata
      481 ttctacccat ttcaatcatt aaaaaattga gagaaaaccc agacacaatt gcggaacgat
      541 tcgaagattg cacagttctt ttctctgaca ttgtagggtt tactcaaatg tcgaagacaa
      601 tgccggcagt ctcaatcgta aaacttttaa atgaaatctt ctctcgattt gatgacctag
      661 ctgaaaaata taagcttgag aaaattaaaa cgataggcga cgcttatatg gtagtcggag
      721 gacttccgga gccagacgaa gatcacgcaa aaaaagtagc aagcttcgca ttagaaatgt
      781 tagaaattat aaatgactat agaaaagaga aaaatattcc aatagaagtt cgaattggaa
      841 tcaattcggg agccgctgtt gccggtgtga ttggtaaaaa gaaattcatc tacgatctat
      901 ggggggatag cgtaaacact gcatcaagga tggaatccca tggcgttcct ggtcatattc
      961 aagtctctga atctacctat cgccacctca aggatctttt tcaatttaaa gatcgaggtg
     1021 tattagaagt aaaaggactt ggtgctgtcc aaagttatct attaacggca aagataggtt
     1081 aattttaatg tatatagcaa aaatactttt caggacgccg aataggatta tgctatcagt
     1141 gtgaatatga atactatatt agaggagctt actgaatttg gagaattatt gtgtttaaaa
     1201 ccaaaaataa aaattgtatc tccaagtcta aaggataaat ccttcttttt tatcaaaaat
     1261 ggctatttgc aaaaatacta ctctataaaa ggtaaaaagc atgttttccg tttgattgaa
     1321 tcaggtgatt actgtgaatc atttacagtt cttcaaggag agaaaaataa ttatgaatat
     1381 attgaaacgg taagcgatac cgtcataata aagtttgatt atcaaaaatc gattatcaaa
     1441 attaacaata gtttaatgct ttccaatttt tttaaagatt tattgagcaa ttttttattc
     1501 tttcaagaga aacgaatttt aaatttttta gcgttaagcc caaaagaaag gtataaagaa
     1561 ttcattgaaa ccaattatca aaaaattgga aaatatccag atcatataat tgcctcttac
     1621 ttgggtataa caaaagaaac attcagcaga ttccgtaata gaaaaagttg atttggatca
     1681 actccttcat tttcaaaaat gattgatcta agagcagttg tattcattga ttcaatgggg
     1741 gtaaaaaaaa tggaaaattt atattttggt atcgcagttt cgtttttatt cattcatgaa
     1801 ttagatgcaa taaaagcgag ggaatggaaa ttatttatat ttttaaaaga tatgtctgaa
     1861 gataaagcct actcagtttg gttagcttta cactttccac tgtttctatt tccgatgatg
     1921 atttattcta aatttttttc tcctgtatac caatatagaa ttcaatttgg attcgacatt
     1981 tttctaatcg ctcatttatt tttgcatatt ctatttagaa aaaataaaag ttactatttt
     2041 acattgattc aatccaatat atatatccta ttgactgctc tgttcgcaat agcggatatc
     2101 gtttctctaa aaggctaaat ctaagcaact cacgaaaact caatcgtttc tcttattgac
     2161 aaatgattga ggcaagattg gatcaatcat ttgtctaaag taagaatgac tgattttatc
     2221 ctgaattcca aactttgatg ctgcctcttt cggactgtag taactaccga aaataagatc
     2281 cataatagga gagagatttg cataattgca tttaccctgt gtttccaagt catgatgcca
     2341 atgatgcaaa tgcggagagc caaaaagaat ttctaaaaag ccgagtctta tatttacatt
     2401 gctatgaatg aacaatgccc aaagtcctcg gaacgttatg tatcctgcaa tctgattcaa
     2461 atcaaatcca agtaggacag cgggaagtgt ttcaagacca cgggtataaa tattatccaa
     2521 aggatgctct ctgaaagccg ctaggtagtc taccgtttcc gccgtatgat gaactcgatg
     2581 aaatctccaa agaaattcat atttatgctg aaatcgatgc ccccaataaa tgaataaatc
     2641 acttagcagg ataacctcta ttatctgcaa aaaaaagttt tgtctcgaaa aatagctttg
     2701 aagcgattga acaaaactaa attccatcca attgaaaaac tgatttaaaa tataaacagt
     2761 tacaaaattc caaaccaatg cctgccctaa ataaaataat aaatctgtaa gccattcctt
     2821 tcgataaata gggcttatcg tttgatagaa acttctttct aaagggataa aaacaattga
     2881 gattccaata aaaaagagga aaacagatgt taaatgctcc ataaaaagaa gctagtttcg
     2941 gtttggtaaa gccggcgctt tagaagaagg cataattgga taatcaatca cttccttttt
     3001 ttcttttttt gcttcctgct ttgactggtc ttttttatcc tcttcaaaaa ttactgtatc
     3061 ttctttctgc ttagcttgct tttcttccgg tttctctttt gcttttttag aaggaaccat
     3121 acttttacta gaatgctgta tcttcttctc tgatttgttt ttcttcgatt ccgcttgaga
     3181 gtagagcacg cttatcgtaa gaccggccac cgctaataag aaaacaaagc cttttacaaa
     3241 tgatttctga aaaactgttt caaatttaga catatatatc accctttcta tttttccaat
     3301 ctaccaaccc aactctattt tggatattct ttaaaaaagt aaaagaattg aaccacgaag
     3361 aaataagaag atattacaat accaagcctc attttctccg tgcgcttctc ggtcaaaaat
     3421 gactcaactg aatcacatag atcgactgag tcgttacaaa aaaaatagat tgatttagat
     3481 tccataaaat tagaaaagta gagagtagaa taatttcaca attcaaaggg agtaaatatg
     3541 gcagtagcag ttcaaggcgg aagttccggc ggagcaagtg gtggcgattt agtaaaaaaa
     3601 ttattggatg caactcgatc cacaaatcct gttggcaatg ataaattaag tgaagcacaa
     3661 ctcaaagtag cagcagttca cattcaagcc atattaaatg aatgtgcaaa agaaggagtg
     3721 actgataaag gtcagatcgc ttatattcta ggaacagtcc gcagagaatg tgttttaggt
     3781 aataaaatga tcgaagtaat cgatgaagcc aaagcaaata caaattattc aaaagaaaaa
     3841 atgccagctc tcggaaatgg agatattgcg tcaggcgatg gattccgtta ccgcggaaga
     3901 ggttatgtgc aaatcacagg tcgaggaaat tactctaaat ggagtcaaaa acttggtatt
     3961 gatttagtgg gagatccaga caaagcttgt gatccagcca ttgctgcaaa aatttgcatc
     4021 gttggaatga gagatggttt atttgcacct gcagcaggac cactttcaaa atatataaat
     4081 ggaaatacgc gcgatttttt aaatgcaaga gctgttgtaa atgggggaga attaaaaaga
     4141 gctcctgaaa aagctaagta tatcgtagcc tatgctgaga gctattacaa agctctgacc
     4201 acataattct attttaagtc aatcaagcgg ttaagactta ttagccgctt cactatggat
     4261 ttaagatttt gttatttaag tatataccat ttgcaattct actcactcta ataaattgct
     4321 acacttcgaa agatcaagtt gataaaagtt atttaatccc actcttgaaa caaacaaacg
     4381 aaaaacagag tattttacaa gtcaatatca taggagattc cttatctgaa agatcagtcg
     4441 gatttggatt acaaaataaa ttaggcaaca actacaaagt gaatgacttt tccgtgagtg
     4501 gtcgttctgt atacagttgg ctattcgaca ttagccgccc atttaaccct caaccaaata
     4561 tattaattat agaattggga accaatgatg ttatgaatga tccaagttat gattttattg
     4621 gttacttcaa tcgattactt accgaaatca agctaagatc gagtgctaga atcattttaa
     4681 ctgccgttcc actcacggat gacaaagggc tacaaaataa aatcaaagta aataataact
     4741 ttatcagaag tctatctgct aattatacag taactgattt ggaagcagtt ttcgaagcca
     4801 atcgctctac aataaaatta tatcctagct atgatccaat tcatcccaat cctgttggct
     4861 atgaactgat aggagaggca tacaaaaaag acattttata tgtgaaataa ctcaccatct
     4921 tgaactaaca ctatgtaaga ttgatcattt aaggaacaaa tgcgaattct gagatgattg
     4981 taaaatcaag caaccaatcc tctttgacat ttcctaattg taagagaatc ctccttacct
     5041 tccttttcgg actctagaat cgatttccat ttttgaaaag aatcggagaa acccttatct
     5101 gaacgtttgg ctcaacttgg atcaaaagag ttactgaaaa actaagttga ccttagatgc
     5161 gagtggataa ctctatcttt gttgaggtat ttcatgtcta aaaaatttct tcctttttta
     5221 ctcttaatct ttgtatttac aaactctgct tttgcaaatg aagcagcaat tctatccact
     5281 gctaaaaagt tagttggata tatccgtttc aagaaaaatg accaagcgat cgcacttatc
     5341 gatacaaaaa ctttttctaa aaacttactt ttaaatcact gggaaaaaat agatcctaaa
     5401 gatcaggcgg attttgaaga agcaatgaaa ggatatatta aagcaaaatc ctttccgatc
     5461 gcactccaat attttgataa aatagatatc aattatgaaa aagtaaaaat gaaaggtagc
     5521 gaggcagaac ttcccgcttc catcctctac aaaggctctg aaaaaattac attctcttgg
     5581 gtatttactg aaaaaaatgg aacctaccta gtgagtgatt ttctattagc agaaggaaaa
     5641 cttgcttcgg aagtaaaccg agtaaaacaa attgagccaa catacgaaaa aaaaggaatg
     5701 aaggaattaa tcgctttaat caaaaaagca tctaaataaa aatgaaaaat ttaatttcga
     5761 ttctctgtca cattttttta cttagaccat ttataacgtt agtcgcatta gttcttctaa
     5821 ttggtctatc catccaacaa gctcttaagc ttagtatcaa cagcaaccaa ctcgacctcc
     5881 ttcctgctag tatgattgaa gtcattaaag caaaagatgt agtggaaatg attggaggaa
     5941 atggtttcta cattgttgct ctaaaaatca aagatgaata tgctagagac aaaaagatat
     6001 tatccgctgt cgatgctaag aaaaaaggaa atctagatct ttataaatca gaaatggaac
     6061 aagctgagaa aatcaagcag gaccatctag attattatac taaaagtgaa aagaagatta
     6121 aacggtatgc tgacaaactc acttccgaac ttctaaaaga taaagatatt agatacatat
     6181 cctacaaata tgatactact ttcttaaaag aacgtttacc tttgtatatc aaatcaagcg
     6241 atatgcgaga agcgagaagt cgtatcaaaa gaaaaataga tgaggaaatc gaaaagctca
     6301 atccatttta tataaatctc acaggagaag aatatacgcc aacctttgat gatatactct
     6361 ccaaatatca gagacttgcc aaaagagatg tatttgatgc ttataatatt tctcccgaca
     6421 aaggaatgct attattactt gtcaagccag agggatcttt cctagattta aactttactc
     6481 gtaatctaga agttaagatc aaaaaagtag tagctgattt aaagctagaa gacaagggaa
     6541 ttcatgttgc ttatagtggg tcctataaat taaacttaga tgattatgat agtttagtag
     6601 atgcgttaaa accaatttcg atggcttctc ttatcggaat tacaatactg ctctttttat
     6661 ttttcagaaa tcctctcttt attttgattc tagttatttc tcttttaaca ggcattgctc
     6721 tcacgtttgg agtaaccggt tttgtaattg gtagactcaa tacagtaaca actatcatgg
     6781 cggctgtttt aatgggtctt ggtattgatt acggaattca attcttatat cgttttagag
     6841 aagaattcac tctgcgtgat gatttaatga cgtcagtcac tgagactatt tttcatactg
     6901 gaattgcttc tctgatttca gcgcttacta cgacatctgc ctttgtagtt ttgatgttct
     6961 ctgactttaa aggatttagt gaatttgggc tgatagcttg ttatggaatc atactcatcg
     7021 ctctatctat gtattttgta acagccttac aaattgcgat catatttaga ctttttccgt
     7081 ccttcaaaag attctttgtt tggagtcaaa aagagagcga agaatctagc tttgctaata
     7141 gattttttgc aaatccaaga ttagtattac gagttagtat agcgattatt attgttatat
     7201 ccctatttgc tccgacagta aaatttaatt atagtggtcg agatttgctt ttggagaatc
     7261 aagaatctct tttgatctat gatgagattg gagatcgatt tgacgttagt tctgatcctc
     7321 aagcaatcgc aacggacact ctagaaaaaa gtgaagctgt ttttgatttc tttacacccg
     7381 ttcctgaaca tatgcaaaac tctgtagatc aagttgtgtc tatttggaat atcattccac
     7441 cagaaaacca acaaaaagaa aatctacata tactaaaaca aatcaaagat gatttaaaat
     7501 atataaaacc acatatgttg acagaggaac agcaaaagca tttaccaacg gtagataaat
     7561 acctaggtgt aaaagacttt agttattatg atgtcccatt tctttttaca aatcaattta
     7621 ctgaagttcc tacaagtaag gtaaaagggc atatgctatt catctatcca aaagtggctc
     7681 tttggcatgg aaaagatttg atcgattttt atgataaagt tgctcaattt gaatatccac
     7741 tgatcagcaa aagaacgtta aatgcaattc tatactctac gaatattgat ctagataaaa
     7801 atagtgatga tcatattatt gcgaaatata cgaaggaaga agaagagaca attctaaaaa
     7861 ttgcaaattc tgcttcaaaa gaagagttaa tggctatgaa tatattgcct ttaacagctc
     7921 aactcattgt agacaaacga ccatttacct caattgaac
//
LOCUS       DAOQOS010000192         7620 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_5350_length_7620_cov_6.506147, whole genome shotgun sequence.
ACCESSION   DAOQOS010000192 DAOQOS010000000
VERSION     DAOQOS010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 7620)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7620)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7620
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5350_length_7620_cov_6.506147"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            60..488
                     /locus_tag="PLF66_24505"
     CDS             60..488
                     /locus_tag="PLF66_24505"
                     /inference="COORDINATES: protein motif:HMM:NF015509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HMZ67271.1"
                     /translation="MVTFPPFIGFLLAIFTSQLEFSFLVKNVLQRLGDTLAPLALFSV
                     GIQFKNANFGSIQRELYLGLFLKMILAPLFIFTLYLCLVKNMSIVAKVSVFEAAMPPM
                     ITGGIVAMENNLEPELASMMVAVGIIISTITLPIWKYVLS"
     gene            complement(513..941)
                     /locus_tag="PLF66_24510"
     CDS             complement(513..941)
                     /locus_tag="PLF66_24510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000643583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HMZ67272.1"
                     /translation="MAQYQPQGIAPNGRPYSVIIAEPSKFQLKQLQQILESEGYKIIG
                     IAETGRDLINLYKENRLVDLIFMEVSLPVMDGYAAFWEMKEQGGILPKIFFISEENSP
                     GVIKSLLDNGAIDYMVKPIKREKVLEKAKIAIEKVNPTFK"
     gene            complement(943..2028)
                     /gene="leuB"
                     /locus_tag="PLF66_24515"
     CDS             complement(943..2028)
                     /gene="leuB"
                     /locus_tag="PLF66_24515"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020763978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HMZ67273.1"
                     /translation="MKKVAVLAGDGIGPEVMKVALSVLKKAISKSGVEFSFTEAPVGG
                     AAIDLTGKPLPDDTLKLCENSDAILFGSVGGPKWETLPPDLQPERGALLPLRKHFDLF
                     ANLRPAIIYTELRNASPIKSEIIGDGLDILILRELTSGIYFGKPKGREGSGAEEFAYD
                     TMRYSRREIERIARVAFQSARKRNKKVTSIDKANVLTTSVFWREVVVALHKKEFSDVE
                     LNHLYVDNAAMQLIVKPKQFDVVMCENMFGDILSDEASIITGSIGMLPSASLSESGFG
                     LYEPSGGSAPDIAGKGIANPIAQVLSAALMLRYSFSMEKEALQIENAVREVIKQGFRT
                     GDIAEKGAKVLGTEEIGKQIELVIEKG"
     gene            complement(2094..3320)
                     /locus_tag="PLF66_24520"
     CDS             complement(2094..3320)
                     /locus_tag="PLF66_24520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008589826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HMZ67274.1"
                     /translation="MNDLSLTQDKLSEIKDRYSKYIVGNYAPYDVAFKYGVGELLFDT
                     NNKQYIDLLCGVAVTNLGHSDADIIDVIRRQADMLFHTSNWFYSEEASLLAEAIIVNS
                     FAGKVFFCNSGTEANEAAFKLARRYAYSKGKHNPVMISLKQSFHGRTVASMAMTGQSK
                     VRDGFGELLSGFDYVEVNDEVSLEDAFERNQGNIAGLILELVIGEGGIIPLTQSFVNL
                     ARKLTQENDALLIFDEIQTGMGRTGKLFACEHYGIEPDAMTLGKALGSGFPIGALVVS
                     TPFETVLNPGSHGSTFGGNHLATAIAYETMRILIGREIIQNVPALSEFIFTRLRMLQG
                     KYPIIKEVRGLGLHIGVDFTIPSKSIAVECLKEGLIVNSTAETVIRIMPPLNIGIERL
                     DEALQIFERVIAKHIQ"
     gene            complement(3313..5067)
                     /locus_tag="PLF66_24525"
     CDS             complement(3313..5067)
                     /locus_tag="PLF66_24525"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003003593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular transferase
                     activity, phosphotransferases [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HMZ67275.1"
                     /translation="MIQAWTEAPFSSDVRAAANEVLAQFKKGESNSEIEAFTIPLEFG
                     TGGIRGVIGSGIGRMNEYTVGRAALGLCRFLKAKYKNKKTTLVIAYDSRRRSREFAEV
                     TAGIAAKHKINVQIFPVVTPTPMLSFAVRHLKAQSGVVVTASHNPPEYNGFKAYLADG
                     GQLVAPDDAKIIGTINKISDWNEVPIVKKTDPDYKKYVKNVDKKVFAAYIAKVLATPI
                     YNQKLKSKDRDYNIVYSPLHGTGGEYMKTLLNKAGYKKVHLVPEQAKPDGEFPTVKFP
                     NPEEKEALVLAEAYARKMNAEVFVATDPDADRMGVGIRRKDGGYELLNGNQIGSIMCA
                     YLCEKVAGQKKFDYHIFKTIVTTDLQEKIAIHNKIKLKNVLTGFKFIGNEMTKIEKDK
                     KKKFLFGGEESYGYLPVDLLRDKDSLSSTLLLLEIITEKKDILSYLNEIYMKYGLYME
                     SLKSITLKGEAGKQKIKESLERLRNTNTELVGSTIGKRKIIGFIDYKNKKSNGQVPKN
                     IFAGLPSSDVIQLVLSGSGKLTIRPSGTEPKIKLYSSFLSIAQPHSVNEIERMKKELV
                     DEIKESETLFVKLAGLNE"
     gene            complement(5194..5718)
                     /locus_tag="PLF66_24530"
     CDS             complement(5194..5718)
                     /locus_tag="PLF66_24530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67276.1"
                     /translation="MNEDFVEIDEYISMKGLIVIPNYTKTEGLELVNSILDKNYYAQK
                     FLSLTTLSDQIIKRFIETQKYFTVDILNSPVIEFSLGYQEENLKRRGRVYYTKNKIGN
                     GSPKKNELFLQMADDFFKWIRKNFKNVKLKGYEEFLISERTMQWLNESNDRKLGDFPE
                     VLKQDIENRKLRVA"
     gene            complement(5839..6078)
                     /locus_tag="PLF66_24535"
     CDS             complement(5839..6078)
                     /locus_tag="PLF66_24535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67277.1"
                     /translation="MSEKKVPNPYGKKGGERHQNLQTKVGEEVEQKGLERIFEHFIKL
                     FNGKRRFIDVAGLDKVNRKEIEFHQIGLQTKKACL"
     gene            complement(6071..6388)
                     /locus_tag="PLF66_24540"
     CDS             complement(6071..6388)
                     /locus_tag="PLF66_24540"
                     /inference="COORDINATES: protein motif:HMM:TIGR02607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HMZ67278.1"
                     /translation="MPAKKKKPKAQTPIHPGVALEKLLNENHLTVYRLSKEIKIDPSL
                     LSKIMKGDRPISPLVSIALGKFFGVDEAYWISLQVQYDLGITKLEKDVLIHSIRTIDE
                     MHV"
     gene            complement(6442..7146)
                     /locus_tag="PLF66_24545"
     CDS             complement(6442..7146)
                     /locus_tag="PLF66_24545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67279.1"
                     /translation="MLFCASAKRYVANRGNDLTDIIDIGLEKDVYGFNIITIVPFFGV
                     SYNESGKGVGLRHSHIGFYKTGDPDNEFVIEDLYSKEKSTFSFNLGNSFFASTGFHES
                     IDPRNLRTQNKNYVVVTLNKGCLKSKKSHYKGKTRYCHWNDNVAITPIEISLGLYFGF
                     RAGFNVGKFVDFFGGIVGYDLIKDDYDENGNLPPIVSKDLEDNIIIPPTPTPEKKGWE
                     SKLDALDENINKRQTK"
BASE COUNT         2204 a         1466 c         1259 g         2691 t
ORIGIN      
        1 atattctttc ttttgtacaa tccttatttt aggcgacttg acaaagtcgc attaagagaa
       61 ttgtaacctt cccacccttt attggatttc tattagcgat ctttacttct cagttagagt
      121 tttcattttt ggtaaagaat gttttacaaa gattaggaga tactttagca ccccttgctt
      181 tatttagtgt agggattcaa ttcaaaaatg caaactttgg atcgattcaa agagagttgt
      241 atttaggttt gtttttaaaa atgattcttg ctccactttt tatttttact ctctatcttt
      301 gtttagttaa aaatatgtcg atagttgcta aagtatctgt ttttgaggca gcaatgcccc
      361 caatgataac aggtggaatt gtggcaatgg aaaataatct agagccggaa cttgcttcga
      421 tgatggttgc ggttggtata ataatatcaa caattacttt accaatctgg aaatatgttt
      481 tgagttagag acggctaatt agagttagcc gtttacttaa atgttgggtt tactttttcg
      541 atggcaatct ttgctttttc taaaaccttt tctcgtttaa ttggttttac catgtagtct
      601 atcgcaccat tgtctagaag acttttgatt acacctggag agttttcttc tgagataaag
      661 aaaatttttg gtaggattcc accttgttcc ttcatttccc agaaagcggc gtatccatcc
      721 ataaccggaa ggctgacttc cataaaaatt aaatcaacaa gtcgattttc tttgtagaga
      781 ttgattagat ctctacctgt ttctgcgata ccgataattt tatatccttc ggattctaaa
      841 atttgttgga gttgttttaa ttggaactta gaaggctcag cgataattac gctgtaaggt
      901 cttccgtttg gtgcaattcc ttgaggttga tattgagcca tattatcctt tttctattac
      961 caattctatt tgtttaccaa tttcttccgt gccgagaact tttgcgcctt tttctgcgat
     1021 atctccagtt ctaaagcctt gttttatcac ttctctcacg gcattctcaa tttgcaatgc
     1081 ttctttttcc attgaaaaag aatatcgtaa catgagtgcg gcagagagaa cctgtgcgat
     1141 aggatttgca attccttttc cagctatgtc aggagcggat cctccggatg gttcatatag
     1201 accaaagcca gattctgaga gagaagcaga agggagcata ccaatagatc ctgtaatgat
     1261 ggaagcttca tctgataaaa tatctccaaa catgttctca cacatcacaa catcaaattg
     1321 tttcggcttt acaattagtt gcatagctgc gttgtccaca tagaggtgat tcaattctac
     1381 atcggagaat tcttttttat gaagagcaac cactacttcg cgccaaaaaa cagaggtagt
     1441 aagaacgttt gctttatcaa tacttgtaac ttttttattt cttttacgag cagattgaaa
     1501 tgcaactcga gcaattcttt caatctcgcg tcttgaataa cgcattgtat catacgcaaa
     1561 ttcttctgcg ccagatcctt cacgtccttt tggtttacca aaataaatac cacttgtcaa
     1621 ttcacgaagg attaaaatat ctaaaccatc tccgataatt tcagatttaa ttggagatgc
     1681 atttcttaat tctgtgtaga tgattgcagg acgtaaattt gcgaataaat caaaatgctt
     1741 tcttaaagga agaagggctc ctctttctgg ttgtaagtct ggaggaagtg tttcccattt
     1801 cggaccaccg acagatccaa ataaaattgc atctgaattt tcacagagct tcagagtatc
     1861 atcaggaaga ggctttcctg taagatcgat agccgctcca cctacaggtg cttctgtaaa
     1921 tgaaaactca acgcctgatt tagaaattgc tttttttaaa accgagagag caactttcat
     1981 tacctccgga ccgataccat cacctgctag aactgctact tttttcataa ttttttgcta
     2041 tgccgattcg aagaatgttt tgaatctctg gatcggcttt tacttcctaa tttttattgt
     2101 atatgttttg ctataactct ttcaaaaatt tgcaatgctt catcgagtct ttcaattcca
     2161 atattgagag gcggcataat tcgaatgaca gtctctgctg tggaatttac aatcaagccc
     2221 tctttgagac attctactgc aatacttttg gaagggattg taaaatcaac tccgatatga
     2281 agcccaagac ctcgaacttc tttgataatt ggatactttc cttggagcat tctaagtctt
     2341 gtgaaaataa attctgaaag cgctggcaca ttttggataa tttctcttcc aatgaggatt
     2401 cgcattgttt cataagcgat agctgtagcc aaatggtttc ctccaaaggt agaaccatga
     2461 gaacccggat tcaaaacggt ttcgaatgga gtggatacaa cgagtgcgcc aatagggaat
     2521 cccgatccta atgcttttcc taaagtcatt gcgtcaggct ctataccata gtgttcgcaa
     2581 gcaaataact ttcctgttct tcccatacca gtttgaattt catcaaagat gagaagagca
     2641 tcattttctt gggtaagttt tcgagctagg ttcacgaagc tttgggtgag aggaatgatt
     2701 ccgccttcac cgattactag ttctaggatt agacctgcaa tgtttccttg atttctttca
     2761 aatgcatctt ctagagacac ttcatcattt acttcaacat aatcaaagcc agaaaggagt
     2821 tcgccgaacc catctcgaac tttactttga ccggtcatag ccatggaggc aaccgttctt
     2881 ccatgaaagc tttgctttaa gctgatcatt acgggattat gctttccttt ggaatacgca
     2941 taacgtcttg ctaatttaaa ggcagcttca tttgcctctg ttccagaatt acaaaaaaat
     3001 acctttccag caaaactatt tactatgata gcttctgcaa ggagagatgc ctcttctgaa
     3061 taaaaccagt tagatgtatg aaagagcatg tctgcttgtc tgcggataac gtcaattatg
     3121 tccgcatcac tatgccctaa attggtaact gctacaccac aaagaagatc aatgtattgt
     3181 ttgttgtttg tatcgaacaa taattcacca actccatatt taaaagctac atcgtaagga
     3241 gcataattcc caacgatata ctttgaatat ctatctttga tttcagataa tttatcttga
     3301 gttaaactta gatcattcat ttaaacctgc caattttaca aataacgttt cggattcttt
     3361 gatttcatct actaattctt ttttcattct ctcaatttca tttacagaat gaggttgagc
     3421 aatcgataaa aaggatgaat ataatttgat cttaggttct gttcccgatg gacgaatggt
     3481 aagctttcca ctaccagaca gaacgagttg aattacatca gaggaaggaa gtcctgcaaa
     3541 aatattcttt ggaacttgtc cgttggattt tttgttttta taatcaataa atccgatgat
     3601 ttttcgcttt ccaatagtgc ttcctactaa ctctgtattt gtatttcgta agcgttctaa
     3661 actttctttg atcttttgct ttccagcttc accttttaga gtgatagatt ttagggactc
     3721 catgtaaaga ccatatttca tataaatttc atttagatag gaaaggatat ctttcttttc
     3781 tgtgatgatc tctagtaaaa ggagtgtgga agaaagagaa tccttatctc gaagcaaatc
     3841 tacagggaga taaccataag actcttctcc accaaaaagg aattttttct ttttatcctt
     3901 ttctatcttt gtcatttcat tgccaataaa tttgaaaccg gtaagaacat tctttaattt
     3961 gattttatta tgaatggcga tcttttcttg taagtctgtg gtaactatcg tcttgaatat
     4021 atgatagtca aatttctttt gccctgctac tttttcacag agataggcac acatgatact
     4081 accaatttga tttccattta atagttcata gcctccatcc tttcttctga ttccaactcc
     4141 catacgatct gcatcaggat ctgttgccac aaaaacttcg gcattcattt tacgagcata
     4201 agcctctgct agaactaagg cttctttttc ttcaggattt ggaaatttga cagttggaaa
     4261 ttctccatct ggttttgctt gctcgggaac aaggtgaact ttcttgtagc ccgctttgtt
     4321 taaaagagtt ttcatgtatt caccacctgt tccatgtaac ggtgaataaa cgatattgta
     4381 gtctcgatct tttgatttta atttttgatt gtaaatagga gttgcaagaa cttttgcaat
     4441 gtaagcagca aaaactttct tatcaacgtt cttaacatat tttttataat ctggatctgt
     4501 ttttttaaca attggaactt cattccaatc agagatttta ttgatagttc ctataatctt
     4561 tgcatcgtca ggcgcaacaa gctgaccacc atcagctaag tatgctttga atccattgta
     4621 ttctggcgga ttgtgggaag cagtaactac tactcccgat tgtgctttta aatgtcgaac
     4681 agcaaaagaa agcattggag ttggagtaac aacgggaaag atttgaacat tgattttatg
     4741 tttcgcagca atacccgcag taacttctgc aaattcgcgg gagcgtcttc ttgagtcata
     4801 cgcgatgaca agagttgttt ttttgttttt atattttgct tttagaaatc gacaaagtcc
     4861 tagagctgct cgaccaactg tatattcatt cattctacca ataccggaac caataactcc
     4921 tctgatcccg cctgttccaa attcgagtgg aattgtgaat gcttcaattt cggaatttga
     4981 ctcacctttt ttaaattgcg ccaacacttc gttagccgct gctctgacat cagaagagaa
     5041 tggagcttct gtccaagctt gaatcatttc ttctttggta aatgttttat tggtgttttt
     5101 tgccataacg tcctccatag atttccattt ctgaaaatcc ctacatacaa atgtatttta
     5161 gaatgtcatg gtgtcaagtg aaggaatttt tggttaagct actctaagtt ttcgattttc
     5221 aatgtcttgc ttgagaactt ctggaaagtc acctaacttt ctatcgtttg attcatttaa
     5281 ccattgcatg gttctttctg aaataagaaa ttcttcgtaa cctttcaatt taacattctt
     5341 gaagttctta cgaatccatt taaaaaaatc atccgccatt tgtaaaaaaa gttcattctt
     5401 tttcggacta ccattcccta ttttattttt ggtgtaataa actctaccac gtctttttaa
     5461 attctcttct tgatacccaa gcgaaaattc tattacaggc gaatttagaa tatcaacagt
     5521 aaaatatttt tgagtctcga taaatctttt tattatttga tctgacaatg tagtaagtga
     5581 taagaatttc tgtgcatagt aatttttatc aagtatagaa ttcacaagct ccaaaccttc
     5641 tgtcttcgta taattaggaa taacaatcaa acctttcata gaaatgtatt catcaatttc
     5701 tacgaaatct tcattcatta aataaaagtt aatctgacga cccatcattg tcaatatcct
     5761 cctctacgtt gtaagcatga aattcaactt gaatattttt ataattttct actccctcta
     5821 ttacatctct ttcttgtttc acaggcaagc cttttttgtt tgtaatccga tttggtgaaa
     5881 ttcaatttct tttctattta ctttatctag acctgctacg tcaataaatc tacgcttccc
     5941 attaaacaat ttaatgaagt gttcaaaaat gcgttcaagt cccttttgct caacttcctc
     6001 gcctactttg gtttgtaaat tctgatgcct ctcgccaccc tttttaccat atggattggg
     6061 aacttttttc tcagacatgc atttcatcta tcgtgcgaat agaatgtatc aaaacatctt
     6121 tctccaattt tgtaattccc aaatcatatt gaacttgcag actaatccag taagcctcat
     6181 cgactccaaa gaattttcca agagcaatag acactaatgg ggaaatcggt ctatctcctt
     6241 tcatgatttt actaagaaga gatggatcga tttttatttc tttggaaagt cgatagaccg
     6301 tgagatgatt ctcattcaat aacttttcaa gtgccacccc ggggtgaatg ggagtttgcg
     6361 cttttggttt tttctttttt gctggcatag ttgtagtcta tataatagtc catttggaca
     6421 tgaattatca aggatgaaat ttcacttcgt ctgcctcttg ttgatatttt catctaacgc
     6481 atctagtttg gattcccaac cttttttttc tggagtcggg gtgggaggta taattatatt
     6541 atcttctaaa tccttgctga ctattggagg taaatttcca ttttcgtcat agtcatcttt
     6601 aattagatca taaccaacaa ttccaccaaa aaaatcaacg aactttccca cgttgaagcc
     6661 tgccctaaat ccaaagtata aacctaagct aatttcaatt ggtgtaatgg ctacattatc
     6721 gttccaatga caatatcgag ttttaccttt atagtgactc ttttttgatt ttaaacaacc
     6781 tttattcaag gtaacaacca cataattttt attttgagtt cttaaatttc ttggatcgat
     6841 agattcgtga aaacccgtcg aggcaaagaa cgaattacct aaattaaatg aaaaagtcga
     6901 tttttccttt gaatacaaat cttctatcac aaactcattg tctggatccc cagttttata
     6961 aaatccgata tgactatgcc ttaaccctac gcctttccca ctttcattat aactgacacc
     7021 aaaaaatgga actattgtaa taatattaaa accataaaca tctttctcta gaccaatatc
     7081 tattatatca gttaaatcat tccctctatt ggctacatac cgtttggcgc ttgcacaaaa
     7141 tagcatttga gagatgagaa atattattgt tagtcttaag ataattagtt tattgaattt
     7201 tattttactt ctaaaaaatt cacacatatt gttccttttg ctctaaggaa tagtttatgg
     7261 taggaataaa aaatcgtcta taggtaaatt ccatgattag agaaaatgcc tatgagttga
     7321 agatgaactg atgctgctgt caagactacc caatttcgct caatgtcatt taagactgtc
     7381 accttcgaaa tcttttatcg gcggtttcta ggactaaaag gtatacagac tcccgataaa
     7441 agatttcggg agtgacatct ttaggaataa cagtctcaat ccataacccg aaacaccgat
     7501 aaaaaatgat agttaactat cattcgtttc tttatcgtta agatatcggt gcgcatcggt
     7561 ggtaaagtct tataaaccag gattgatcat actttctggc tttacccatg ctttgaattt
//
LOCUS       DAOQOS010000193         7496 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_5491_length_7496_cov_5.933208, whole genome shotgun sequence.
ACCESSION   DAOQOS010000193 DAOQOS010000000
VERSION     DAOQOS010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 7496)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7496)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7496
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5491_length_7496_cov_5.933208"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            238..492
                     /locus_tag="PLF66_24550"
     CDS             238..492
                     /locus_tag="PLF66_24550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004503550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4160 domain-containing protein"
                     /protein_id="HMZ67280.1"
                     /translation="MPTVFNRNGFRFFFYSDEMSGNRLEPVHIHVKKEKYEAKFWINP
                     VSLAYNEGFSRSDLREIEWLILENTNLIQEKWDEHFKGLK"
     gene            506..769
                     /locus_tag="PLF66_24555"
     CDS             506..769
                     /locus_tag="PLF66_24555"
                     /inference="COORDINATES: protein motif:HMM:NF021873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
                     /protein_id="HMZ67281.1"
                     /translation="MKPNVKFEEVKIIDANGVWARISDGREITIPTSWFKRLVGAPKE
                     KLNKFELTPFGIHWEELDEDISINAFIFGLPEISYTEQLTASV"
     gene            766..1032
                     /locus_tag="PLF66_24560"
     CDS             766..1032
                     /locus_tag="PLF66_24560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67282.1"
                     /translation="MKIKLKNGSFIEVDELFIESSKESEALLNKIKSFSLTYISDACK
                     KHPNGKEGIANALNRDIKFLTKLYDDGSVMQAFRIAKQLEDMGL"
     gene            1049..1246
                     /locus_tag="PLF66_24565"
     CDS             1049..1246
                     /locus_tag="PLF66_24565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67283.1"
                     /translation="MDSKTGKNWIICTRKEAFVAEAKRLSFAVSVETYSAIEANAKKE
                     NRSIANYMRTVIEKLLAKKEK"
     gene            complement(1396..1818)
                     /locus_tag="PLF66_24570"
     CDS             complement(1396..1818)
                     /locus_tag="PLF66_24570"
                     /inference="COORDINATES: protein motif:HMM:NF014599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051262 - protein tetramerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HMZ67284.1"
                     /translation="MEQKKTEPKILIKTISLVDSSFHREAVQGPNFEDNLALDREVQL
                     TEDKKLGIVFLTAKLDRKNTQNNNIEVAISVKYVGIFEAESDSDMDLQRFCEINAVAM
                     LYPYIRNHIQETFLKASVSQIILPPINIHTTFPENKSN"
     gene            complement(1822..2340)
                     /locus_tag="PLF66_24575"
     CDS             complement(1822..2340)
                     /locus_tag="PLF66_24575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67285.1"
                     /translation="MKLTTLYSMFLVIKEIEAKFNTSAFIYYDDSDKDYSIAISNVDL
                     YLSNLFREYVSNVAKKSNITVISLPRTLLEGCISFKDLSELFGETNPIHITEKVHSGS
                     ASSMRIDYLQTSETYPFNIDHVEIKNQGDRKINIKAKSNQTSLDYQTQLTSTFECKIE
                     SINNLQLMEIAA"
     gene            complement(2506..2727)
                     /locus_tag="PLF66_24580"
     CDS             complement(2506..2727)
                     /locus_tag="PLF66_24580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67286.1"
                     /translation="MKKKSEDNIKAGKLCKENQMYSAAINIYYYSILQLMLYILDKKG
                     LWSEEDAEKANTHSNAKEKILKLIKEKIL"
     gene            3675..4415
                     /locus_tag="PLF66_24585"
     CDS             3675..4415
                     /locus_tag="PLF66_24585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67287.1"
                     /translation="MAKPFQYDFWGRIYPERTYVTISPSLEFRAKILEKNIEITLRIS
                     INVAQITVSILSESEIKDIFTVKNIVEDFLYSIINSFGYSQGSAFTIEITGVINQQSG
                     KTIIFSVSIPVLIEQVKNLENQFSDVINHLLITNDWRLRLCLADLCSSIKFSKDSAFL
                     VYRGIESIQSYFADKHSIEKKQAWEKFREELQIEYSELMFVKDFADPIRHGGLKPISD
                     QDRGKVLMIGWAIVDKYISYRRQEVTFS"
     gene            complement(4766..5188)
                     /locus_tag="PLF66_24590"
     CDS             complement(4766..5188)
                     /locus_tag="PLF66_24590"
                     /inference="COORDINATES: protein motif:HMM:NF014048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease NucS"
                     /protein_id="HMZ67288.1"
                     /translation="MLEKDFEDILEIHPYLLDPSFKKYKTLRQKKIRKGRVDLFLISD
                     DNLNIVIIEIKRDKLILSSLSQIKRYYYYFRRKYKGTNIIISAYLVGTSSNLDISRLK
                     WLKIKLIDKDIPRKIKLCPKCNTAFDYKVFTCKCGFRV"
     gene            complement(5192..5785)
                     /locus_tag="PLF66_24595"
     CDS             complement(5192..5785)
                     /locus_tag="PLF66_24595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67289.1"
                     /translation="MPNSDFEDLKKSVAELKKEIDHLFNSIQGSNKLDEFKSIQTFEK
                     KISKLSQLTSLKEIKAILKKIIQSQKETQKKLSQKDIQKFIQLQTDYEKFTTRVLGSD
                     KLSIKELQTLIHLNNSSLDISKKINQLVDERRLNIQSWSDEKITSEFNNTSKYPSLES
                     IKNVLSDGLIDKKANTREKLIKSILKKLKTFRASEIK"
     gene            complement(5794..6693)
                     /locus_tag="PLF66_24600"
     CDS             complement(5794..6693)
                     /locus_tag="PLF66_24600"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HMZ67290.1"
                     /translation="MAYVISIVNFKGGVGKTTIAVNLATTLAKDFTKRVLLVDFDAQM
                     NATTHILSIVDRYDELFTNGKTIYHAMFKKKFNREAINLDDYILKETFKNNNDLTLVP
                     NLHLLAGATNMVDFESILSTYQKNNGLPFLFLNELLSTIHGNYDFIIIDTSPYLGNET
                     KNSILASNGYIVPLIPEPFSKNGLNVLLNKIHSFSTKHPTYKNTFTEFLGVVFTHFRS
                     RVTDENSFIDWCRDSFNSKHILNYGIQPGAKNNIFNTYLSYRPKPYRKSVSENLPLFK
                     AKDSSTAVSEFHDLAEELLDKIK"
     gene            6943..>7496
                     /locus_tag="PLF66_24605"
     CDS             6943..>7496
                     /locus_tag="PLF66_24605"
                     /inference="COORDINATES: protein motif:HMM:NF025157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic/DOC family N-terminal domain-containing
                     protein"
                     /protein_id="HMZ67291.1"
                     /translation="MDIKNFKSGTFQKGFQYEYFLPEKINHPFYWSEESLNELLEKAS
                     LKLGELNSFSRLFPDTDMFIVMHIFKEAVLSSRIEGTQTNIEEALVDEKDIDPEKRDD
                     WNEVQNYVAAMNGAIQELKNLPLSNRLIRNTHKILLASVRGMHKNPGEFRESQNWIGG
                     ASLADAVFIPPIHTELPELLSDLE"
BASE COUNT         2526 a         1044 c         1242 g         2684 t
ORIGIN      
        1 tagtctcctt ttggaagcct aaaatcgtgt ccttttttta tgtaggggtt caaagacaac
       61 acgaagaaag ggattagaga gtaaaggaag gaagttagtt aagaaaagta ttctaattgg
      121 ttttacttaa tattgcaata gattttctat gaatgcaaca aatagtgcta gaataaaaaa
      181 agttttaaag aagaatggac attagagggt tgcgacctac cgcaaggaga aatttttatg
      241 ccaacagttt ttaaccgaaa tggtttccga ttcttttttt actctgatga aatgagtggt
      301 aacagattgg aaccggttca tattcatgtg aaaaaagaaa agtatgaagc caagttttgg
      361 attaatccag tttcattagc ttataatgaa ggattttcga gaagtgatct acgagagatc
      421 gaatggttga ttcttgaaaa taccaatcta attcaagaaa aatgggatga acattttaag
      481 ggtttgaaat agaatggagg ataagatgaa accgaatgta aaatttgaag aagttaaaat
      541 tatagacgct aatggagttt gggcaaggat ttctgatgga agagagatta ccattcctac
      601 ctcttggttt aaaagattgg taggtgcgcc aaaggaaaaa ctaaataaat ttgaattgac
      661 tccatttgga atccattggg aagaattaga tgaagatatt tcaattaacg cttttatctt
      721 tggacttcct gaaatttcct atacagaaca attgactgct tctgtatgaa aattaaatta
      781 aaaaacggtt cattcattga agttgatgaa ttgtttatag agtcctccaa agaatcggag
      841 gctctcttga ataaaataaa atccttctct ctcacttaca tatccgatgc atgtaaaaaa
      901 catccaaatg gaaaagaagg cattgccaat gctctcaatc gcgatattaa atttttaact
      961 aagctttatg atgatggatc tgtaatgcaa gcatttcgta ttgccaagca attagaagat
     1021 atgggtcttt aattgttagg gctgttaatt ggattctaaa accggaaaaa actggataat
     1081 ttgtacccgt aaggaagcct ttgtagcaga agctaaacgt ctttcttttg ccgtatctgt
     1141 ggaaacctat tctgctattg aagcaaatgc taaaaaagaa aaccggtcga ttgctaacta
     1201 tatgcggact gtgattgaga agctgcttgc gaagaaagag aagtaggggt aaggtaataa
     1261 aagtaatttc ttttggcaat caatgcgaca ttgccagctt agttaaaatt ttacatctac
     1321 taaagacata gtgctacttt ttagtagtca ccggcaaaat acaaagataa atctatttta
     1381 ttgaataaaa aataattagt tacttttatt ttctgggaaa gttgtatgta tatttattgg
     1441 aggtaaaatt atttgagata cagatgcttt taaaaatgtt tcctgtatat gatttctaat
     1501 gtatggatac aacatggcta ccgcatttat ttcacaaaat ctctgtaaat ccatatccga
     1561 gtccgactct gcttcaaata taccgacata ctttacgctt atagcaactt caatattatt
     1621 gttttgagta ttctttctat ctaacttcgc agtaagaaaa acaattccta attttttatc
     1681 ttcagttaat tgaacttctc tatctaatgc tagattatct tcaaagtttg gtccctgaac
     1741 agcctcacga tgaaaactac tatcaactaa agaaatagtt tttatcaata ttttgggttc
     1801 agtttttttt tgttccatat actatgcagc tatttccatt aattgtaaat tatttataga
     1861 ttcaatttta cactcgaaag tagaagttaa ttgtgtttga taatctaaac ttgtttggtt
     1921 cgattttgct ttgatattga tctttcgatc accttgattt ttaatttcaa catgatcaat
     1981 attaaaagga taggtttcag atgtttgaag ataatctatt ctcattgaag atgcagaacc
     2041 tgaatgaact ttttcagtaa tatgaattgg atttgtttca ccaaataact cagagagatc
     2101 tttaaaagaa atacaacctt caagaagtgt ccttggaagg ctaataacag ttatattcga
     2161 ttttttagcg acattagaaa catactctcg gaataaatta ctcaaatata gatctacgtt
     2221 agaaatggca atgctatagt ctttatcaga atcatcataa tatatgaacg cactagtatt
     2281 aaactttgct tcaatttctt ttataaccaa aaacatacta tataatgttg ttagcttcat
     2341 tgcttttctc cttttaaatt agttttaaaa gatctgtctg catattatta ataattggga
     2401 taacttgatt gcaatcattt atatcaaagt cttttttatc ataatcagcc ttttctctag
     2461 taagtctaat tattttaaag tcacgagtaa atgaagttgg ctcttctaaa gtattttttc
     2521 ttttatcaat ttaaggattt tttctttggc atttgaatga gtattcgcct tttctgcatc
     2581 ctcttcagac cataaacctt ttttatctaa aatataaagc atgagctgta agatagagta
     2641 ataatatata tttattgccg ctgaatacat ttgattttct ttacaaagtt taccagcttt
     2701 gatattatct tcagacttct ttttcatgtc agaatgaatc ataatactgt atgtatttca
     2761 tgatttccct gagtatttta ttttgcaagt gataattatc ttttattctt agactttgta
     2821 ttggaggatt ttatagatag agagtatagt gagaaattaa tggaaatctt taatttgatt
     2881 ccttttcttc tggtagaatt aatttaaacc cgctgaagta tctaaatata aaggagtaat
     2941 ctctgccact agaatctttc ctctctatct tcttggatca tctttgcaag attatggata
     3001 cgaatctttt tttggtttcg aagaagtagg aaagaggaaa tatctgtttc ttttaaggta
     3061 acaatccatt ttctttcccc aaaaaacctt cctgaaacat cttactttcc cattcaaatt
     3121 acaagaattt tgtagtaaaa tacattttcc attgacagaa aaattctggt aattagcctc
     3181 tattttatcc tagtgtatga ctatatagga tttgattgat cgagcagatg atgagattga
     3241 tgcgttagtg tatataagtt gtatgggttg acggaagagg agattgggac tgtggtggga
     3301 cgggatagtg agtcaaaact ataaaaatac atttcaaata cagtatcttg caaatataaa
     3361 aaagtaattt gcaaactggt attacgatat aaagagccaa attgataaaa atttactgga
     3421 gttatcaaat ccatgttcag acaaagagtt tttcttaatc agaaaattgg aactcgaagg
     3481 gttggttttt gcgtggagaa tgttgtttcg ctcgagttaa aagcgattac tcgactggaa
     3541 gatgttcatt atgcgcaggc tattatttaa aagcctataa tttgtaaatt ggatattgat
     3601 taactttgga gagacaagtt tgaattttaa gtggctgacg aataagaagt ttggtaagga
     3661 tatgaaagta aattatggct aaaccatttc aatatgattt ttggggacga atctatcccg
     3721 aaagaacata tgtaactata tcaccatctc ttgaatttag agcaaaaata cttgaaaaaa
     3781 atattgaaat aactttaagg ataagtatca atgtagctca aattacggta tcaatcttaa
     3841 gcgaatctga aataaaagat atttttacag ttaaaaatat agtagaagat tttttatatt
     3901 caataattaa ttcattcggt tactcacaag gtagcgcatt tacaattgaa attacaggtg
     3961 taataaatca gcaatcggga aaaacaatta tattctccgt atcgattccg gtattaatcg
     4021 agcaagtaaa aaatttggaa aatcaattta gtgatgtaat aaatcatcta ctaatcacaa
     4081 atgattggcg gctaagactt tgtttagcag atttatgctc tagtataaaa tttagtaaag
     4141 attctgcatt tcttgtttat agaggaattg aatctataca aagttatttt gcggataaac
     4201 attcaataga aaaaaaacaa gcttgggaaa agtttcgaga agaattgcaa attgagtatt
     4261 cagaattaat gtttgtaaaa gattttgcag atccaattcg acacggcggg ttaaaaccga
     4321 tttctgatca agatagaggt aaagtattaa tgattggttg ggcaattgtg gacaaatata
     4381 tttcttatcg tagacaagaa gtaacattta gttgaggtaa tggtaaatag aagctgatga
     4441 ttgatattct cccttatctg aaatcaccta gtgaaaagac ttttgttaaa ttgaacaaaa
     4501 caatttcttc tcagagcgac tttaaaaaag ctacacagaa ttaaataatt acaaagtaaa
     4561 aatagttcga ttcgcaagat ctcaggatct accaaaagca ataagtgata gaatgagaga
     4621 tatattaaat tttaaggtca ccacttaaat taataaaatt tgcaatgaat aaactaattt
     4681 taatccttct tttattgact tttctatttt ttactgattt ctaaaagtta gctaaaaact
     4741 aatttgtagg aagagcataa ccacctcaca ccctaaaccc gcatttacat gtaaacactt
     4801 tataatcaaa cgcagtgttg catttggggc aaagtttaat tttgcgcgga atgtctttat
     4861 caattagttt aattttaagc cacttgagtc gtgagatgtc aagattagaa cttgttccta
     4921 ctagataagc agaaataatt atgttagttc ctttatactt tctacggaaa taataatagt
     4981 atcttttgat ttgggaaagg gaggataata tcaatttatc tctcttaatt tcaataatca
     5041 caatatttag attatcatct gaaattagaa ataaatctac tctgccttta cggatttttt
     5101 tctgtcttag agttttgtat tttttaaaag aaggatctag aagatagggg tgaatctcta
     5161 gtatatcttc aaaatctttt tctaacattt tttattttat ttcggacgct cgaaatgttt
     5221 ttagtttctt taggatactc ttgattaatt tttctcgcgt atttgctttt ttatcaatca
     5281 atccatcaga gagaacattt tttatagatt ccaagcttgg atatttagaa gtattattaa
     5341 attcggatgt aattttttca tcactccatg attgtatgtt tagtcttctt tcgtctacta
     5401 gttggtttat tttttttgat atatctaatg atgaattatt tagatgaatt aatgtctgca
     5461 attcttttat agataactta tcactaccta atactctggt tgtgaatttt tcataatcgg
     5521 tttgtagctg aatgaatttt tgaatatctt tttgggataa ttttttctgt gtttcttttt
     5581 gggattgaat tatctttttc aatatagctt ttatttcttt taacgaagta agttgactga
     5641 gtttacttat ttttttttca aaggtttgga tgcttttaaa ttcatctagt ttatttgagc
     5701 cttggattga attgaacaaa tgatcaattt ctttttttaa ttcggctacg gattttttta
     5761 aatcttcaaa atcagaattc ggcatatcat ctcctatttt attttatcta aaagttcttc
     5821 tgctaaatca tgaaattctg aaacagcggt agaactatct tttgctttaa aaagtggtaa
     5881 gttttctgat acactttttc tatacggttt tggtctataa ctaagataag tattgaaaat
     5941 attatttttt gcaccaggtt gaattccata atttagtata tgtttactat taaagctatc
     6001 tctacaccaa tctataaatg agttttcatc cgtcactcgg ctacgaaaat gagtaaacac
     6061 tacacctaaa aattctgtaa atgtattttt ataagtagga tgttttgttg agaacgaatg
     6121 aattttattt aaaaggacat tgagtccatt ttttgagaat ggttctggaa ttaagggaac
     6181 aatataaccg ttacttgcca agatagagtt tttagtttca tttcctaaat aaggagatgt
     6241 gtctatgatt atgaaatcat aatttccatg tattgtcgat agtaattcat ttaaaaataa
     6301 aaaaggtaga ccgttgtttt tttggtatgt tgatagaatt gactcaaagt ctaccatatt
     6361 tgtagcacca gctaaaagat gaagattagg aactagcgtc aaatcattgt tattcttaaa
     6421 agtttctttg agaatataat catctaaatt tatagcctct ctattaaact tctttttaaa
     6481 cattgcatga tagattgtct ttccattcgt aaataattca tcataacgat ccactataga
     6541 tagaatatga gtggttgcat tcatctgtgc atcaaagtca actaagagaa cccgtttagt
     6601 aaaatctttc gctagggtag tagctagatt taccgcaatg gtggttttac caactccgcc
     6661 tttgaaattt actatactta tcacgtaagc catactttag aaagtgaata atgacctcca
     6721 tttgtctaga ataaatatag tgtatattaa aaatagtaag catctcttaa aaagtaaagg
     6781 gaattgattt tagatctcat taaaaacctt caatctagcc caacagactt atttaaatcg
     6841 gtaaatttca attgaatgaa gaaaagtaaa ataaaatttc ttataattta ctttaatgac
     6901 gaaaagtaat ttatattttc ttaagcatac tttagaggct caatggatat taaaaacttc
     6961 aaatctggga catttcagaa aggtttccaa tatgaatatt ttcttccaga aaaaataaac
     7021 catccattct attggagtga agaatcctta aatgaacttt tggaaaaagc ttctttaaaa
     7081 ctaggcgagc tgaactcttt ttcccgttta tttccagaca cagacatgtt tatcgtaatg
     7141 catattttca aagaggcagt tttgtccagt cggatagagg gaactcaaac gaatattgag
     7201 gaagcgctag tagatgagaa agatatcgat ccagaaaaac gggatgattg gaatgaagtt
     7261 caaaattatg ttgcagcaat gaatggagcg attcaagaat taaaaaatct tcctctatca
     7321 aatcgattga ttcgaaatac gcataaaatt ctcttagcca gcgttagagg tatgcataaa
     7381 aatcctggcg aattccgtga gtctcaaaat tggattggtg gtgcttcttt agctgatgct
     7441 gttttcattc cgcctattca cactgaattg ccagaattgc tttctgattt ggaatt
//
LOCUS       DAOQOS010000194         7409 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_5598_length_7409_cov_5.516998, whole genome shotgun sequence.
ACCESSION   DAOQOS010000194 DAOQOS010000000
VERSION     DAOQOS010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 7409)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7409)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7409
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5598_length_7409_cov_5.516998"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            242..1645
                     /locus_tag="PLF66_24610"
     CDS             242..1645
                     /locus_tag="PLF66_24610"
                     /inference="COORDINATES: protein motif:HMM:NF015043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid oxygenase family protein"
                     /protein_id="HMZ67292.1"
                     /translation="MKWQNLFNGTKKEFEATKLKLVSGELPKEIKGDFYINTSVMTTR
                     GGKQNGSWFDGDGAVLRVHLENGEAEGTYKYTRTKVFVEEEKADKFLYPTVSNLAPGF
                     FNRLKKSWKPANRANTSVYPLSDKLMALFDGDTPYELDLKTLETKGESNFNGAIGENE
                     GSGAHIRIDPNTGELFSFGFDNKDYNNIILYKFGKDQKFLYKNKIKTASPPLMTHDIV
                     FAGDYILFWVFPLKIDILPMFLKLKPPIDSYYWNQKGNSHIYVVRKKDLSLVAEIKSD
                     ANYFLHFINGFQSENGALIVDLIEFNNFDVFTKWSKDAVTGNLPDYEVFSTLVRYELD
                     INEFKVVNKSVLHKDSSYEWPLVKLNLQGKPYDHFFICHGKEDMLTNQFMKMEISSGK
                     VTNRKFGPNSYCNEPSFIPSKKNTNFGYLILVVYNGELDKSFVHILDEEKLEDVCVFE
                     LPSPIGNCSHGKWKSLV"
     gene            2086..2961
                     /locus_tag="PLF66_24615"
     CDS             2086..2961
                     /locus_tag="PLF66_24615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012387456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67293.1"
                     /translation="MIKYFFWALAFLNISMMYANDTTQYTPEQANWKTFQSKPLAAGN
                     TGHDPDSVTWIKKEHWDSSKWDGKTIYDPTKMTKAEFFAAICPSADRVRGIREVFYAH
                     KPFKDNKNPTKAEVDEWHRIAINHIRALVGYTTPDRQVQKDQCMFKRALWGDERKFTT
                     KWDAKYPGKLGSAEGPCQGSKNAHCGASFIPSPEDQAPYFSETVLVCKTQAGAEGVFS
                     GPKSNIPWSLKWSRGFCNTLAAEGFWGGHVGPWFHREKFGFSFWDSDEKNNNSNAVLR
                     AKWTGKLMPSLYTKP"
     gene            complement(3129..7391)
                     /locus_tag="PLF66_24620"
     CDS             complement(3129..7391)
                     /locus_tag="PLF66_24620"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HMZ67294.1"
                     /translation="MTSYTRSEKLKKEEIEKIENFCEHVSGAIYNSLLLKQTEKAQRE
                     TEKQKRETEELNKLIKSLNEDLNIQVIMKKVYEYLKENHKIHYYGLGVIDDKEEKIIT
                     IDTLSPEFLTDEERLRVSQFSTKINEKIGGHAFAYRSGKPFYIPKIRKGAMTEEEQYN
                     QRVTKLESLLIIPLILQGNFIGFLDLYNVGRMELDKDDLNKLSILGEQLAGIIYSSSL
                     LKKIQEEKRNADNTRQEIEKLNEFTRLINSTSDLSLIMNEVYSYLNQNFEITNVWTLL
                     VDKNSNEFYTNENLSIYRGLESIDNNYFLSFRESINEKLGTMYQIFNTKNPIYISDFT
                     KTIKGTKNEYFNEINGEIISGSKIDLKIILKGKLKTTLQLPLIVQDEVIGIISLSSPN
                     TILKLTNIDLEKLIRFSNQIAGVIQNAQLLKETEKAKLEADLEKENAIVAQKETEKAR
                     KEIEKLNEFTKRINEKYDLDEMLDLVGEYIVTNFPISHYLLWKKDFDGKSLSPYKGTY
                     SKDIPEENIKKFKELKIPLEYDEGVHASVCKNKKYLFMRGIKRHKSLSNIENTINELL
                     DFDSLLVIPLINQNQVIGTMDFSNYSGKMSISKDEIKRIGIFCEQVSGVIHGAGLLKQ
                     AEEAKKNALVAQLETEKQKQETEELNKLIKSLNEELDLKVIMQKVLQYVNLHFHIQYY
                     ALYKSNKQNTHVEMEEANFPVHMTEENRINVLNLKIPIKNVKGVHAFPFKAKRPFFIP
                     RLRESGGTEEENFIKRVYNISSIIVIPLILNNDIIGTIDFSSEQKLQLSKDDITKLSI
                     LGEQLAGIIHGSNLLKQVQEEKEKSESIRLEIEKLNELTKKINSTSDLDSILEIVFNY
                     FEKEFGLLGFILGINNSTNQIHCLKTSSLEFSPEEVKFASNIQETLGPELGSIWAGIV
                     NKKTLYINRYNEKFLTDFDRNLITNLKLKGHFQVPLIIHDEVIGIAMFTDRREKFAIS
                     KEVRKSVEQFCEQIAGAIYNAQLLKETEKARKETEGLNKLMKNVNSVSSLIDIMAFIM
                     YYLEKQLQFREFSLLLNEETKQELSVFSFTSPRASKEQNDYFKNWKHKYRDLSGVFEK
                     VVEEKKIVFCSKEDLDRDDTIARDWIDRGEFTYIILVPFIVYDELIGVLFVHANQKEY
                     SITKEILTKLEKFSDLVAGSIFNAKLLKKVEEAKEEADTERNKSEKLLLNILPKDVAK
                     ELKEKGFAEPSVFESVSVMFTDFKGFTTIAETMSANELVKELDACFVQFDKITERYNL
                     EKLKTIGDSYMCAGGIPKKNKTHAVDCVLASLEIQNFMNLMKQLKEERGFPYWELRLG
                     IHSGPLVAGVIGERKFAYDVWGDTVNTASRMESSGTPGMINISGSTYQIVKDFFECEY
                     RGKVNAKNKGEVDMYYVHGLKPEYTKEDGRTPNGKFWELYGG"
BASE COUNT         2302 a         1299 c         1126 g         2682 t
ORIGIN      
        1 ggggaaagtc gagtctgcga gactttggga acgggggcag tctggggggc aagtggtcgc
       61 tggttcaaat ccaatcactc cgagtttttt tcaaatctct ctaaattttt aactcccttt
      121 acaattccct aaagaatcaa actttgacct tttacagtga ttgcatcgat ctctaatgag
      181 agtaaatctt gatagccata gaaatagaat atcaaatatt ctaactcaaa aggttaaatt
      241 tatgaaatgg caaaacctat tcaatggaac aaaaaaagaa tttgaggcaa caaaactcaa
      301 attggtctca ggagaattgc ccaaagaaat caaaggcgat ttttatatta acacatcggt
      361 tatgacaacg cgtggtggaa aacaaaatgg ctcttggttt gatggagacg gtgcagtcct
      421 tagagtgcat ttggaaaatg gagaagcaga aggcacatac aaatacacac gaactaaagt
      481 ttttgtggag gaagaaaaag cagataaatt cctttatcca acagtctcaa atcttgctcc
      541 aggttttttt aatcgactaa aaaaatcttg gaaaccagca aacagagcga atactagcgt
      601 ttatccgtta agtgataaat tgatggctct ttttgatgga gatactccct atgaacttga
      661 tcttaaaacc ttagaaacaa aaggggaatc taattttaac ggagcgatag gagaaaacga
      721 aggttcagga gcacatattc gaattgatcc taacacgggt gagctcttta gttttgggtt
      781 tgataataaa gactacaaca atatcattct ctataaattt ggtaaggatc agaagtttct
      841 ctataaaaat aaaatcaaaa cagcctcccc tcctcttatg acgcatgata tcgtttttgc
      901 tggagattat attctttttt gggtatttcc tttgaaaatt gatattctcc caatgttttt
      961 aaaattaaaa ccacctatag attcttacta ctggaatcaa aagggaaact ctcatatata
     1021 cgttgtccgc aagaaagact tatcacttgt agcagagatt aaatccgatg cgaattactt
     1081 tttacatttc atcaatggtt ttcagtctga gaacggggca cttatcgttg atttaattga
     1141 gtttaacaac tttgatgttt ttacgaaatg gtctaaagat gcagttactg gaaatttgcc
     1201 cgactatgaa gttttctcta cacttgttcg atatgagcta gatatcaatg aattcaaagt
     1261 agtaaataaa tctgttttac ataaagactc ttcttatgaa tggccgttgg taaaattaaa
     1321 tttacaaggc aaaccgtatg accatttttt tatctgccat ggaaaagaag atatgttaac
     1381 caatcaattt atgaagatgg aaatttcttc tggtaaagtg acaaacagaa aatttggtcc
     1441 aaactcctat tgcaatgaac caagttttat tccttccaaa aagaatacaa attttgggta
     1501 tttgattctt gtagtttata atggagaact agataaatcc tttgtgcata tactcgatga
     1561 agaaaaacta gaagatgtat gtgtatttga actaccctct cctattggaa attgctcaca
     1621 cggaaaatgg aaaagcttag tttaacgata aattgttcac tctttttata acaattaaat
     1681 ctattgtaga atcgattttt gtttttgcgg ttaatcgttg tttatctctt ctattgattg
     1741 aactttatag atgtctaaga atgaatcatt tagaaagagt attttggaaa gaaaaaacaa
     1801 tcccttttta atgagggatt tcacatttca attatataga agatgcctct tgattttttt
     1861 ttacaaatca tacttccctt ttcccaaact atatttccag agcccaccat cacatttaaa
     1921 tagtgtcgca tcaaaacttc catttacttc tcctttctat tttattctac aaacagaagt
     1981 acaaaacgag tgatacaatc taaatacatt ttttaaagct tgtaataaat cagaaacaat
     2041 ttcctactaa actcatctta aatagatttt taaaggaatt actctatgat taaatatttt
     2101 ttttgggcgt tagcatttct aaacatttcg atgatgtatg caaatgatac aacacagtat
     2161 acacccgaac aagcaaattg gaaaacattc caatcaaaac cgttagctgc gggaaatacg
     2221 ggacatgatc cagatagtgt gacttggatt aagaaagaac attgggattc ttctaagtgg
     2281 gatggaaaaa cgatttatga cccaacgaaa atgacgaaag cagaattttt cgcagcaatt
     2341 tgtcctagtg cagatcgtgt gcgtggaatc agagaagttt tttatgctca taaaccattc
     2401 aaagataata aaaacccgac gaaagcagaa gttgatgaat ggcatagaat tgcgatcaac
     2461 catattagag cgttagttgg atataccact cctgatagac aagttcaaaa agaccaatgt
     2521 atgtttaagc gtgctctttg gggagacgaa agaaaattca caaccaagtg ggatgcaaaa
     2581 tatcctggta aacttggatc tgcagaaggt ccttgccaag gctccaagaa tgctcactgt
     2641 ggagcgagtt ttattccaag tccagaagac caagcaccgt attttagtga aacagtttta
     2701 gtctgcaaga cgcaagctgg tgcggaaggt gtattcagtg gaccaaaatc aaatattcca
     2761 tggtcgctca aatggtctcg tggattttgt aatactctcg ccgcagaagg tttttggggt
     2821 gggcatgtag gtccatggtt tcaccgtgaa aaattcggtt ttagtttttg ggactccgat
     2881 gaaaaaaata acaatagcaa tgcagtctta agagcaaaat ggactggaaa attaatgcct
     2941 agtctttaca ccaagcctta attaaataca attcgatagc tgtttctcta gtttaaattc
     3001 tcttctaacg gctatcgtct atttctaaac tctttttatt ggcaaaatca ccaaatagta
     3061 aagaattctt gaaaatattt ttagcctcat gaaatatttc tctagatttc ttataaaaat
     3121 ttgtggtatt atcctccata caattcccag aatttaccat taggtgttcg cccatcttcc
     3181 ttcgtatatt caggtttaag cccatgcaca taatacatat ctacttctcc tttgtttttt
     3241 gcgtttactt tccctcgata ctcgcattca aaaaagtctt ttactatttg ataggtagac
     3301 ccactgatgt ttatcatccc aggtgtgcca ctcgactcca ttctacttgc tgtattcact
     3361 gtatctcccc aaacgtcata agcaaactta cgttcaccga taactccggc taccagtgga
     3421 cctgagtgga taccaaggcg taattcccaa tacggaaatc ctctttcctc ttttaattgt
     3481 ttcattaagt tcataaaatt ctgaatttcg agcgaggcta gaacacagtc tacagcatga
     3541 gtcttgttct ttttgggaat tcctccagca cacatatagc tatcgcctat tgtttttaac
     3601 ttttccaaat tgtatctttc tgttatttta tcaaattgaa caaaacatgc atctaactct
     3661 tttactaatt catttgcact cattgtttcg gctatcgttg taaatccttt gaaatctgta
     3721 aacatcacac tcacagattc aaacacactt ggctctgcaa agcctttctc ttttagttct
     3781 tttgcaacat ctttagggag gatattcagg agtagcttct ccgatttatt tcgctctgta
     3841 tctgcttctt cttttgcttc ttctactttt tttaatagct ttgcattgaa tatcgagcct
     3901 gctactaagt ctgaaaattt ttctagtttt gttagtattt cttttgtaat tgaatattct
     3961 ttttgatttg catggacaaa taagactccg attaattcat cgtagacgat gaatggaact
     4021 agaattatat atgtaaattc acctctatca atccagtccc ttgctattgt atcatctcta
     4081 tcaaggtcct ctttagagca aaatacaatt ttcttttcct ctacaacttt ttcgaatact
     4141 cccgataagt ctctgtattt atgcttccag tttttaaaat aatcattctg ctctttactc
     4201 gcgcgaggcg atgtgaagga gaatacggat aattcctgtt ttgtttcctc atttaataat
     4261 aaagagaatt ctctaaactg aagttgcttc tctaaataat acatgataaa ggccataata
     4321 tcaattaaac tcgaaaccga attaacattt ttcattaact tatttaggcc ttctgtttct
     4381 tttcgagcct tttcggtttc ttttaataat tgcgcattat aaattgcacc tgcgatttgt
     4441 tcacaaaact gttcgactga ttttctaact tctttcgaaa ttgcaaattt ttctcttcta
     4501 tctgtaaaca ttgcaatccc aatcacttca tcatggataa taagtggaac ttggaaatgt
     4561 cctttgagtt ttaagttagt aattaaattt ctatcgaagt ctgttagaaa cttttcattg
     4621 taacgattta tatagagagt ttttttattt acaattcccg cccaaataga gcctaactcg
     4681 ggtcctaatg tttcttgaat attcgaagca aatttgactt cttccggtga aaactctaag
     4741 ctagatgtct taagacaatg aatttgattc gttgaattgt taattcctaa aatgaaacca
     4801 agcaaaccaa attctttctc aaagtaatta aaaacaatct ctaagataga atccaaatca
     4861 gatgttgaat taattttttt tgtaagttcg tttaacttct ctatttctaa tcgaatagat
     4921 tcggattttt ctttttcctc ttgcacttgt tttaataaat tagatccgtg gataataccc
     4981 gctaactgct ctcctagaat agaaagcttt gtaatatcat ctttagataa ttgtaatttc
     5041 tgttctgaag aaaaatctat cgtcccaatg atatcattgt ttaagatgag aggtattacg
     5101 atgattgatg aaatgttata aacacgtttg atgaaatttt cttcttctgt tccccccgat
     5161 tccctaagtc ttggaataaa aaatggtctt tttgctttga aaggaaaagc atgaactccc
     5221 tttacatttt ttataggaat tttaagattt aaaacattta ttctattttc ttccgtcata
     5281 tgaactggaa aatttgcttc ctccatttcc acatgagtat tctgtttatt tgatttatag
     5341 agtgcatagt attgaatgtg aaaatgtaaa ttgacatatt gcaaaacctt ctgcattata
     5401 actttcaaat ctagctcttc atttaggctc ttgattaatt tatttaattc ttcagtttct
     5461 tgcttttgct tttcagtttc aagttgagca actaacgcat ttttttttgc ctcctctgct
     5521 tgttttaata atccggcacc atgtattaca cctgaaactt gctcacaaaa tattccaatt
     5581 cgctttattt cgtctttaga aatactcatt ttcccagaat aatttgaaaa gtccatagtt
     5641 ccaataactt ggttttgatt tataagcgga atcactaaca aggaatcaaa gtctaaaagt
     5701 tcattgattg tattttctat atttgaaaga gatttatgac gttttatacc tctcataaac
     5761 aaatattttt tatttttaca aacagaagca tgaacacctt catcatattc taatgggatt
     5821 tttaactctt taaatttttt tatattttct tctggaatat ctttagaata agttccttta
     5881 taaggggaaa gtgattttcc atcgaagtct tttttccaaa gtaagtagtg gctaataggg
     5941 aaatttgtaa caatatattc tcccactaaa tctaacattt catctaaatc atacttttca
     6001 ttgattcgtt tagtaaattc atttaacttc tctatttctt ttctggcttt ttctgtctcc
     6061 ttctgtgcaa ctatcgcatt ttccttttct aagtcagctt ctaatttagc tttttctgtt
     6121 tcttttaata attgagcatt ttgaatcaca cctgcaattt gatttgaaaa tcgaattagc
     6181 ttttccaagt ctatatttgt tagtttcaga attgtattcg gactagataa actaattatc
     6241 ccaattactt catcttgaac tataagagga agctgcaaag ttgtttttag ctttcctttc
     6301 aatatgattt ttaaatcgat tttacttcct gaaataatct ctccatttat ttcattgaag
     6361 tattcatttt ttgtgccctt tatagtttta gtaaaatcag aaatataaat tggattctta
     6421 gtattaaaaa tttgatacat tgtacctagt ttttcattaa tactttccct aaaggagaga
     6481 aaataattat tatctattga ctccaagcct cgataaatgc taagattttc attggtataa
     6541 aactcattcg aatttttatc tactaataat gtccagacgt tagtaatttc aaaattctga
     6601 ttcaaataag aataaacttc gttcataatg agcgaaagat cagatgtaga attaattaag
     6661 cgagtaaatt catttagttt ttcaatttct tgtctggtat tatccgcatt acgtttttct
     6721 tcctgaatct tttttaataa actcgaacta taaataattc ctgccagttg ttctcctaaa
     6781 atggagagtt tatttagatc gtctttgtct aattccattc tacccacatt gtataaatcc
     6841 aaaaaaccaa taaaattacc ttgtaaaatt agaggaatga ttaacaggga ttcaagcttg
     6901 gttactcttt ggttatattg ttcttcttct gtcattgctc cttttctaat tttaggtata
     6961 taaaatggtt ttcccgagcg gtatgcgaaa gcatgtccac cgattttctc atttattttt
     7021 gtagaaaatt gactaaccct taatctctct tcatccgtta aaaactctgg agatagagta
     7081 tctatagtaa taatcttttc ttccttatca tcaatcactc caagaccata ataatgaatt
     7141 ttatgatttt cttttaaata ctcataaact tttttcatga taacttgaat gttcaaatct
     7201 tcatttaaac ttttaataag tttatttagt tcttctgttt ctcttttttg tttttcggtt
     7261 tctctctgcg ctttttcagt ttgctttagt agaagcgaat tgtaaatagc gcctgaaaca
     7321 tgttcacaga aattttctat tttctcaatt tcttcttttt ttaatttttc acttcgtgta
     7381 taagaagtca tcaacacaag tccaaagga
//
LOCUS       DAOQOS010000195         7323 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_5697_length_7323_cov_5.482664, whole genome shotgun sequence.
ACCESSION   DAOQOS010000195 DAOQOS010000000
VERSION     DAOQOS010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 7323)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7323)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7323
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5697_length_7323_cov_5.482664"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1446)
                     /locus_tag="PLF66_24625"
     CDS             complement(<1..1446)
                     /locus_tag="PLF66_24625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67295.1"
                     /translation="MQILNSIENFFFGKVHTVEVKETINTETNSNFHEMLHQAVNDAS
                     KVEQAPLNLYTSVSEKEISNDTAIKSFAMEAPVYKTREEKNLADLKIQETNPKQVEEP
                     FSSNVSYLTSLNTSKEESNFLEDNTNLEMVEEDFKKLESKEQSVETEKSAFSDKLLES
                     FVKSEVKSNVKIEKVSVEQVQDKIEKQSNDLLALENKIVEVSPKKTTQVEDPKKNDSV
                     KNSIPAEMELSHLKAKDIKVSIDTKEMISEKSLSPKIQEKPANGISEDVKNLKVTKNN
                     EAFSNPNKTASIKNESNLELNQINNSLSIKEEVSVFTNTIENKKSKKNFEIDTKEKLN
                     QDNKVASKEAVTIEKDMRPIREGMVDVLSLDRNKWTITRKERELETQLSDRKKDSVLI
                     ANEMAIKSSSSQSSSDSDKSFSNPKYETNGLKTFSETLKAANSDKPKEALFDRENFSK
                     SLNDLVTKARINIVENGKNSAQISLYPKELGK"
     gene            2187..2618
                     /gene="flgB"
                     /locus_tag="PLF66_24630"
     CDS             2187..2618
                     /gene="flgB"
                     /locus_tag="PLF66_24630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015680998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009429 - bacterial-type flagellum basal
                     body, proximal rod [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HMZ67296.1"
                     /translation="MFTDTYFMKTQDLLERGLNNASFKRRILADNIANADVPHFKRSE
                     VTFEANLKRALESEESEKKHAVPTQISHEKHIEFFKPLNYKDVQSKANVDYLTTMRAD
                     GNNVDIEKEMTEVSHNQMAYTIMVERLNQNFRLLNTVIRTV"
     gene            2632..3093
                     /gene="flgC"
                     /locus_tag="PLF66_24635"
     CDS             2632..3093
                     /gene="flgC"
                     /locus_tag="PLF66_24635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004459730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HMZ67297.1"
                     /translation="MGLFTSINISSTGLSAQRLRMDVISNNIANATTTRNTNGDGPYR
                     RDRVVMTPINLRTQWKSPVYPFGISAGEGQGVKVMKVEKDMSPLRLVYDPTHPDAIKV
                     GQKKGYVEFPNVNIVTEMTDMISASRSYEANVQMINGSKAMFNKSLEIGRA"
     gene            3122..3493
                     /gene="fliE"
                     /locus_tag="PLF66_24640"
     CDS             3122..3493
                     /gene="fliE"
                     /locus_tag="PLF66_24640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012387769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HMZ67298.1"
                     /translation="MSNFNISNIGGVYNSGSPYPVSPKGDKVSVHTSDTRHYGDVKAT
                     HSPDDVAESFAQVLQKSFEKVNDQQVEADTLTQKLVFDPNSVDSHQVLIAAEKARISL
                     TFTKTMIDGVIKAYRDLSNLR"
     gene            3587..4465
                     /locus_tag="PLF66_24645"
     CDS             3587..4465
                     /locus_tag="PLF66_24645"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HMZ67299.1"
                     /translation="MSILAIDDEVLNLKLIEKLIKHRYRTLLVQSGKEALAQLEKGGR
                     EIKCILLDWNMPEMNGLDFLKIIKADSRFQRIPVIIQTIDTSKDTLLAGMSAGAFQYL
                     LKPYDEKTLISHVHLAVQEYDRYHELLKNFEHNMMAFTMIESAEFTFNTIDEAQKLAS
                     VIASLYPDPENVVIGISDLFANAIEHGNLNITYEEKTKLLEEGKLLKEIDYRLSLPEN
                     KLKKVRVKFQKSAKEIRLRIIDQGNGFDFQKYLNLDELKLFESHGRGIYLAKKMSFDQ
                     IFYHGNGNDVETVVYF"
     gene            complement(4471..5031)
                     /locus_tag="PLF66_24650"
     CDS             complement(4471..5031)
                     /locus_tag="PLF66_24650"
                     /inference="COORDINATES: protein motif:HMM:NF012774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HMZ67300.1"
                     /translation="MKHIVLFIFLLIPIFNLFANSNKKWEMLNDCKYLENKSNDGDSF
                     HVECDKEYLFRLYFVDTPESDDSFPNRVEEQADYFGISSPESIELGKTAASFTKQALM
                     DKKFTVWTRWKDAMGRSKLNRYYAIIIVDRKSLIELLVENGLARIHGANANLPDGKKS
                     KHYIKKLKTLEAIAKKNGVGGWNQKE"
     gene            5458..6342
                     /locus_tag="PLF66_24655"
     CDS             5458..6342
                     /locus_tag="PLF66_24655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67301.1"
                     /translation="MPECNSYFKYCEGCLHDKACIADSKILSFYEGNLPLSIAYNAFE
                     NANPLFLSSKAKENIELIICLKYFIHISEPTKVAEKLKFFKNDILSMILKANYYEYLE
                     KQNANGGRNFFEIKQNHFVKYFSEEKKLEMIHSLVSEEEFKLASLFLLILSPKYSQRL
                     TEIMPNDNETEKRLYLALEDDVFTLAKINPELYFRLLEILKDDSTGMLEILNEYREIA
                     NFSLASLERVKMIMDEYETVRDKIFPLQWFYTKLKNTSNEERIQIVRLLEQEEKISKA
                     DKDLLESFFSNHSRIFFL"
     gene            complement(6726..>7323)
                     /locus_tag="PLF66_24660"
     CDS             complement(6726..>7323)
                     /locus_tag="PLF66_24660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010572834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alkene reductase"
                     /protein_id="HMZ67302.1"
                     /translation="SENAISAGFDGVELHGANGYLLDQFLNPGSNQRKDEYGGSIENR
                     CRYVIEVAKEVSAKIGKEKVGIRLSPYGAFNDLFPFPETEEEYAYLASELNKLGIAYI
                     HLVDHSSMGAPKVEPSTVKKIRSNFKNTLILSGGYDRKKAEADLDSNAGDLIAFGKPF
                     LANPDLVVRLEKNAGLNQVDMSTFYSADSKGYTDYPTL"
BASE COUNT         2466 a         1243 c         1109 g         2505 t
ORIGIN      
        1 ctttcccaat tcttttggat aaagagatat ttgcgcagaa ttttttccgt tttctactat
       61 attgattcta gcttttgtta ccaaatcatt taatgatttg gaaaaatttt ctctgtcaaa
      121 caaagcttct ttcggcttat ctgagtttgc cgcttttagt gtttctgaaa atgtttttaa
      181 gccatttgtt tcatacttag gattagaaaa agatttatct gaatcacttg aactttgaga
      241 agagctagac ttgattgcca tttcatttgc aataagaaca gaatcttttt tgcgatcact
      301 caactgtgtt tctaattctc tttctttgcg agttatagtc catttatttc gatctagact
      361 taaaacatct accattcctt ctcgaattgg tcgcatgtct ttttctatcg tcactgcttc
      421 tttcgaagcc actttattgt cttgatttaa tttttctttg gtatcgattt cgaaattctt
      481 tttagatttt ttattctcta ttgtatttgt aaaaacagaa acttcctctt tgatagataa
      541 agaattgttt atttggttta attccagatt cgattcattt tttatagatg ctgttttatt
      601 tggattagag aaagcttcgt tgtttttagt tacctttaga tttttaacat cttccgaaat
      661 cccatttgca ggcttttcct ggatttttgg agagagtgat ttctcagaaa tcatttcttt
      721 tgtatcaatc gaaaccttaa tatccttcgc tttcaaatga gaaagttcca tttctgcagg
      781 aatgctattc tttacagaat catttttttt agggtcttct acttgagtcg ttttctttgg
      841 tgatacttct acaattttat tttcaagagc taaaagatca ttggattgtt tttcaatctt
      901 atcttgcact tgttcaactg agactttttc tatctttaca tttgatttaa cttctgattt
      961 aacaaaactt tctaagagtt tatcagaaaa agcgctcttc tctgtttcta cactttgttc
     1021 ttttgattct aactttttga aatcttcctc aaccatctct agattagtat tgtcttctag
     1081 gaaattagat tcttctttag atgtattgag acttgttaaa taggaaacgt tactcgaaaa
     1141 aggttcctca acttgttttg gattagtctc ttggattttt aaatcagcta agtttttttc
     1201 ttctcgtgtt ttatagacag gtgcttccat tgcaaaggac ttaattgcag tatcattaga
     1261 aatttctttt tcagagacgc tagtatagag atttaaaggg gcttgttcta cttttgatgc
     1321 atcgtttaca gcttgatgaa gcatttcatg aaaatttgaa tttgtttccg tgtttattgt
     1381 ttcttttact tcaacagtgt gtactttacc aaagaaaaag ttttctatac tatttaggat
     1441 ttgcattaaa agtccgcctt aatcactaca tcgaacagtt tggaaaaagc ttaattaaaa
     1501 aagtacacga tgtgtactaa tttttccgtt ttctgcattg gacataaaaa tattatccta
     1561 cctataagtg cttatcatac ttattatagc atttatatca ttatccacaa ctgattacca
     1621 ccaacgtttc tgttttttat cggcggcaaa aaatccttat aacgaatcca tgttcttgaa
     1681 ttcagccact atcttagagt agagcgagat tattattcat gagattatcc cccttttgac
     1741 cctctaacta cttggcttag ggcatagtgg ttgatatgtt aaaatgtttc tttgcattgt
     1801 attctacttt cttaactctt aaagattagc acggatgaat acagataaaa tattaagaat
     1861 tattaaaacc atctgcattt ctactgccac tcgctcaata gtatcaaatt atggattctt
     1921 aaccacgaag gactcgaaga tcacaaagag aataaatagt aaaccttctc ttttcttggt
     1981 gttctctgcg ctcttcgtga tctactctct tttatttctc ttaacttgac agtattaccg
     2041 gagactttct tcgatgccgt atcattctca accattcata cttcacaaaa aaatattttt
     2101 tatatcaaag cgacataatt tttttaatgt aaatcgcaaa acctgtcgaa gatagcttat
     2161 ataactatca caagatagga gttggtatgt ttacagacac ttatttcatg aagacacaag
     2221 atttattaga aagaggactc aataatgcct cttttaaacg tagaatcctt gcagataata
     2281 tagcaaatgc tgatgttcct catttcaaaa gaagtgaagt tacatttgaa gcaaacctaa
     2341 aaagggcact cgaatcagaa gaatctgaaa aaaaacatgc tgttccaact caaatttcac
     2401 acgaaaaaca tatagaattc ttcaaaccac tcaattacaa agacgttcaa tctaaagcca
     2461 atgtcgatta tctgactaca atgcgagcgg atggaaacaa tgtggacatt gagaaagaaa
     2521 tgacggaagt atctcataac caaatggcat atacaatcat ggtagagaga ttaaatcaaa
     2581 atttcagatt attaaataca gtcattcgta ctgtctaaga ggttttaaaa aatgggatta
     2641 tttacatcaa taaatatttc atctactggt ctttcagctc aaagactcag aatggacgtt
     2701 atttcaaata acatagcaaa tgctacaacc acacgaaata caaatggaga tggaccttac
     2761 agaagagata gagttgtaat gactccaatt aatcttagaa ctcagtggaa aagtcctgtt
     2821 tatccttttg gaatctctgc cggagaaggt cagggcgtaa aagtgatgaa agtagaaaaa
     2881 gacatgtcac cacttcgttt agtctatgac ccaactcacc cagatgcaat caaagtggga
     2941 caaaaaaaag gctatgtaga atttccaaat gtaaatattg ttacagaaat gactgatatg
     3001 atttctgctt ctagatctta tgaagccaat gtgcaaatga tcaatggaag caaggctatg
     3061 tttaacaaat cattagaaat aggcagagcc taatcaatag aactaaaact tagaggtagt
     3121 tatgagtaat tttaatattt ccaatatcgg aggagtttat aattcaggtt ctccttatcc
     3181 agtatctccc aaaggtgaca aagtttctgt tcacacaagc gatacaagac attatgggga
     3241 tgttaaagca actcattcgc ctgacgatgt tgctgaaagc tttgctcaag ttctacaaaa
     3301 atcattcgaa aaagtaaatg accaacaggt agaagctgac acacttactc aaaagcttgt
     3361 atttgatccc aatagtgttg attcccacca agtattaatt gctgcagaaa aagctagaat
     3421 ttcactaacg ttcacaaaaa ccatgatcga cggtgtgatt aaagcatata gagatttaag
     3481 taatctcaga taatctctaa aacagaaaag gtttgttcaa tctgaaataa attgacaaat
     3541 cttttttcta tattacacta tcatcaatta ggtagaggat tttcctttga gtatacttgc
     3601 aattgatgat gaagttttaa atctaaaact gattgaaaaa ttaattaaac atagatatag
     3661 aacacttctt gtccaatcag gaaaagaagc acttgctcaa cttgaaaaag gtggtagaga
     3721 aatcaagtgc atcctgctcg actggaatat gccagaaatg aatggcttag attttttaaa
     3781 aataatcaaa gcagattccc gatttcaaag aatccctgtt atcattcaaa ccattgatac
     3841 atcgaaagat acacttctag cggggatgtc tgctggagca tttcaatatc ttttaaaacc
     3901 ttatgatgaa aagactctta tctctcatgt ccatttagca gttcaagaat acgatagata
     3961 tcatgaatta ctaaaaaatt tcgagcacaa catgatggct ttcacaatga ttgaaagtgc
     4021 tgagtttact tttaatacaa tcgatgaagc tcaaaaacta gcctctgtga tcgcctctct
     4081 ctatccagat ccagaaaatg ttgtgattgg aattagcgat ttgtttgcca acgcaatcga
     4141 gcacggaaat ctaaatataa cctatgaaga aaaaacaaag ttattagaag aaggtaaatt
     4201 gctcaaagaa atcgactatc gtctctcttt accggaaaac aaattaaaaa aagtaagggt
     4261 taaatttcaa aaatcagcaa aggagattcg ccttagaatc attgatcaag gaaatggttt
     4321 tgactttcaa aaatatttaa acttagatga attaaaacta tttgaaagtc atggtcgggg
     4381 aatttaccta gcaaagaaaa tgtcctttga tcaaattttt tatcatggaa atggaaacga
     4441 cgtagaaact gttgtctatt tttaaatagt ctactctttt tgattccagc cgcctactcc
     4501 attttttttc gcgattgctt ctagtgtttt taattttttt atatagtgct tggatttttt
     4561 accatcaggc aaatttgcat tcgctccatg gattcgagct aaaccatttt cgacaagtaa
     4621 ctctatcaaa ctttttcgat ctacaattat gattgcatag tagcgattca atttgctacg
     4681 tcccattgca tctttccagc gtgtccaaac tgtaaatttt ttatccatca aagcttgttt
     4741 tgtaaaacta gccgcagtct tgccaagttc tatagattca ggagaactta taccaaaata
     4801 atcagcttgc tcttccaccc tatttgggaa agaatcatcg gactcaggtg tatcaacaaa
     4861 ataaagtcga aataaatatt ctttatcaca ttcaacatga aaagaatctc catcattcga
     4921 tttgttctct agatatttgc aatcatttag catctcccac ttcttatttg aattcgcaaa
     4981 aagattgaat atcggaatga gtaaaaatat aaacagaact atatgtttca tattatttac
     5041 tccttagatt ttttcataat tgtcaatcgc tataatagag acttacagaa agatttacta
     5101 atgcaaaaat atttcattta ttgatacaac aatagagcaa ttttgaaccg aatcatttcg
     5161 tatttccagt taaactaacc tgattgaatt ttgcactgcg taaaacccgt acttcttcag
     5221 agcgattgca aaaaattgta aatgtatctc aaaatgaaat atttaatata gattgattct
     5281 taatcttaaa aaattttttg cggcaaattg atattaacca cgttgctttt taataaattt
     5341 ttaagtctca cagaatagta aatgacgatt taataaatta ctgttaaaat ggatataaga
     5401 agaaatggta atttgagaaa ttcaattttc taaaattttc cagaagagac attgaaaatg
     5461 cccgaatgta attcttattt caaatactgt gagggatgcc tgcatgataa agcttgcatc
     5521 gccgatagta agattttgtc tttttacgaa ggaaacctcc ctctatctat cgcttacaat
     5581 gcatttgaaa atgcaaaccc tctatttctg tcctctaaag cgaaagaaaa cattgaactc
     5641 attatttgtt taaaatactt catacacatt tctgaaccta ctaaagtggc agaaaagctt
     5701 aaatttttta aaaatgatat cctatctatg attttaaaag caaattatta tgaatatcta
     5761 gaaaaacaaa atgcaaatgg tggtagaaat ttttttgaga taaaacaaaa tcatttcgta
     5821 aaatattttt cagaagagaa aaaattagaa atgattcatt cattagtaag tgaagaagaa
     5881 tttaaacttg catcgctatt tttgttgatt ctatctccaa aatactcaca gcggctaaca
     5941 gaaattatgc ccaatgacaa tgaaacagag aagagacttt acctagctct agaggatgat
     6001 gtatttacac tcgcaaagat taaccctgaa ctctatttta gacttttaga gatactaaaa
     6061 gacgactcaa ccggcatgct agaaatttta aatgagtatc gagaaatagc aaactttagt
     6121 ttagccagtt tagaaagagt aaaaatgatt atggatgaat atgaaacagt cagagataaa
     6181 atttttcctc ttcaatggtt ctatactaaa ctaaaaaaca catcgaatga ggaaagaatt
     6241 caaattgttc gtttactaga acaagaagaa aaaatttcaa aagctgataa agatttactg
     6301 gaatcctttt ttagcaatca ctctagaatc ttttttttat aaatttcgta aatccaatct
     6361 agattttatt cttagttact gctgttacgc aaaattaata attaaaaaat caatgctaat
     6421 tcatagaata ttagagaatg tgaatgaatt aataaaatca acaaactcga ctgttggaaa
     6481 gcttataaag aaatgattgg acagtctctt cgttccttcg ctttcgctct actcaaaccg
     6541 attctcattt ttcaatgaca aaggtagcaa agcttatgta aagatttgat tcgccttgca
     6601 taaggtgagt tagttagtaa aattttcttc caaatagaca tctaccgatt tacaaatcaa
     6661 ttttgatcat aaaaaataag tatataaatc ggtagaacca agaactgaaa tatctatttt
     6721 atgaattaaa gtgtaggata atcagtataa cccttggagt ctgcagaata aaaagtagac
     6781 atatccactt gatttaaacc tgcatttttt tccaaacgaa ccactaaatc cggatttgca
     6841 agaaacggtt ttccaaaggc aattaaatct cctgcatttg aatctaaatc ggcttctgct
     6901 ttttttcgat catatccacc acttaagatc aaagtatttt tgaaattact tctaatcttt
     6961 ttaactgttg atggctctac tttaggagct cccattgaag aatgatctac taaatgtata
     7021 taagcaatcc ctaatttatt taactccgat gctagataag catattcctc ttctgtttca
     7081 ggaaatggaa atagatcatt aaacgcgcca tagggagata agcgaattcc tactttttct
     7141 tttcctattt tagcagaaac ttcttttgca acctctatca cataacgaca tctattttca
     7201 atactaccgc catattcatc ttttctttgg tttgagcctg gatttaaaaa ttgatccaat
     7261 aaataaccat ttgctccatg cagttcaaca ccatcaaaac cggcgctgat tgcattttcg
     7321 gat
//
LOCUS       DAOQOS010000196         7243 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_5799_length_7243_cov_5.594046, whole genome shotgun sequence.
ACCESSION   DAOQOS010000196 DAOQOS010000000
VERSION     DAOQOS010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 7243)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7243)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7243
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5799_length_7243_cov_5.594046"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3895
                     /locus_tag="PLF66_24665"
     CDS             <1..3895
                     /locus_tag="PLF66_24665"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HMZ67303.1"
                     /translation="QDESFIYFKENEWRWEKEKIYKLNISENVIELMLRKIKKLSDTI
                     QSLLGIAACIGNQFELSILIEIYLEGRESLIKNLETCIQDGLITPVGESYKLFSSGNL
                     ESNYYFEFVHDKIQQAAYQLISDSKKKEIHLEIGRYLLKRSNEKEINESLFDILGHLN
                     YSIDLVHEDEKYNIALLNLKAGKQAKLSIAYKSALQYFQNCLNLLSDKNWNQFYSELF
                     ECIFNLVETEFLLGNYQSAIKYFEIGMDNANSRLDKAKLYNLRIALFNTQGDYQKAIE
                     SGLDGLRLYDFKIPSKINDLVLFKEYLKVKNVLGENPILFIQSLSVSDNQEELEKIKL
                     SLNILDSIYLTNPSLGTFITINAMLSVIKIGNSYISPIVYASFAAFLCNINEYKLGYN
                     LGKYALENISESLKAKCYHIFCSLINHWSRSVRDTFPLQSKGFQIGIETGDVIYACYN
                     AFMYIWNHFFAGVKLDATLKEGNKYFKAVEKLKNRDSEKFILILLNHLREITETRMDY
                     SDSEILEMCEVSQTKTPYFIFYLLKFQKYFITEQYENAAKMIPEILAIQDSVTSTMYL
                     TEFRFYYCLNLFIKDDKSEEANKQIEKELKQWELWAKNCEPNYQHKYFLLQAEWCKAN
                     AKIQEAMEYYDKAIYSSKEYDFIQNSALASKLAGMFYLSLDRGKIADLYFSEAFQKYK
                     NWGSLYYSNFIKSRYLKNFYEPSSSDVISQKVRGTTSSSVNSVYIDINSILKASRIIS
                     SDVRFDSLSKNFLQVIIENAGAERGVLLISESNEFFIYSEANVNGEFNLNKRKQDSSI
                     LPTSVLRYVELTKESVVLHNASNEKRFQRDEFIIEKKVKSMVCLPIMNQNQMLAILYL
                     ENNLFERVFTEERIETIQMLSSQAAVSIQNSILYQSMEEKVKERTNELKTMLDKVNEL
                     KRQQDADYYLTSLLIQPLSNNNLNSRIKIEFLVLQKKEFEFRKESNFIGGDICISDEI
                     VLRKKKMIVFVNADAMGKSLQGAGGTIVLGSVFKSILERTKMDDRLQSQFPELWLKNA
                     FSELHKVFQTFEGFMLVSAIIGLIEEETGFMYFINSEHPHSVLYRDGKASFIPKTQSL
                     FKMGSLGGDDSIVIIQTFQTYPGDIIINGSDGREDLIKDYTEHGKPNVYHEEKLFLDY
                     VEEAKGDLHTIKNLIVNSWELYDDLSLLRVEIPENLEIQNIEKKTLFDFIPDYLSLDK
                     NTKRIQLLELYNQNLKENKNLYLYKLLSKEFVKFRDYEIAANCARIYCESNPSDLRML
                     KFYAFILEKIGEFDEADLYKQRLLLRAHTYSQSRL"
     gene            4233..5528
                     /locus_tag="PLF66_24670"
     CDS             4233..5528
                     /locus_tag="PLF66_24670"
                     /inference="COORDINATES: protein motif:HMM:NF019237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HMZ67304.1"
                     /translation="MKTKRSLFDFSSPAGIGTFFVFSNVPSNQPTNSNTTTETVSSPS
                     FTPQAGTYNSIIDIEIKTTTADAVIYYTLDGTDPTLASNKYTSPISIRTIAGLTLKAI
                     GIKSNFSNSSVTAGIFSLLPLKTGQTTSFSSGDDGNKNLGTLRSFSGPTPHSVYTSDF
                     TTTDNITGLVWKTCAEGSTGSNCGGGSLQSNTWSSAPSYCTSLNSNNSNNGFAGRTNW
                     RITSKQELESIIHYGASNEAIYATNFPTNGQLLGPIWASEEYDISTAWIIDESNGYSF
                     NLAKGNSAVFIRCVSGTAYKYSAILSDNNNGTIQDKRTGLIWQKCSRGQDPANSCSGG
                     ITSDTWTNALSYCNSLTLASKSWRLPNINELKTISNFEATAPKIDSSFFPSTASSQYW
                     SSTTYFPGTSSALVLDFGTGGIIYSPKSNSFAIRCVSGQ"
     gene            complement(5542..>7243)
                     /locus_tag="PLF66_24675"
     CDS             complement(5542..>7243)
                     /locus_tag="PLF66_24675"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HMZ67305.1"
                     /translation="TSLDLFILEVAYANLDEIEFFFTDSKNQLVRKLLGRLHPFSERD
                     FKHRTYLVSLRDIKPKGRFFIRVKTQSSIQMPVFIHNQKLFFEKDQYNFAFQLVLAGV
                     MFSMFLYNLLLGIALKDKVYFFYLSFTLPASLFLMGYNGITAQFLWGDNPWFNRDFAP
                     VLLALNNLGILLFVRSFLKLKINHPKMDRFIKLLIYISALAFIGSWFVRYDLISRTLV
                     FFVIANSLAAIAAGFLLAKEGYRPAKIYLLAWGILLLGALLLALTGFGIVPINVFTRN
                     LAQFGLGLESILISFALADRIKILEQEKEKAQRESISALKKSERLKVELELARKIQQS
                     IIPRRIPNIPGLEIAAWYRPMETVGGDFYDFRTKESNLGFIMADVSGHGVPAALVVSA
                     VKTAFGFQELNIEYPSDLLKSMNEILRDKTGGEFVTACYAYIDMEKKILITGNAGHSA
                     LWIFRKQTGELISLKPKGRALSLTQDSLFDSTEIHLLFGDRILLYTDGLFEAGNHTGE
                     LFGEEKIQELLKLSANMSAKEFGESLLKKVIDWSPGEDKLEDDIALIVVDYLLDSSLN
                     "
BASE COUNT         2520 a         1107 c         1263 g         2353 t
ORIGIN      
        1 gcaagatgaa tcctttatct attttaaaga aaatgaatgg agatgggaaa aagaaaaaat
       61 atataaatta aatatttcag aaaacgttat tgaattgatg ttaagaaaaa taaaaaaatt
      121 atctgacaca attcaaagtt tactcggaat tgcggcatgt attggaaatc aatttgaact
      181 ttctatcttg atcgaaatat atttagaagg tagggaaagt ttaatcaaga atttagaaac
      241 ctgtattcaa gatggattga tcacacctgt tggagaaagt tataaattat tttcttcagg
      301 aaatttagaa tcaaactatt attttgaatt tgttcatgat aagattcagc aagctgcgta
      361 tcagttaatt tctgattcaa agaaaaaaga aatacactta gaaattggta gatatttatt
      421 aaaaaggagc aatgaaaagg aaataaatga atcgttattc gatatattag gacatttgaa
      481 ttatagtata gatttagttc atgaggatga aaaatacaat atagctttat taaatctcaa
      541 agccggcaaa caggcaaagt tatcgattgc gtacaaatct gctttgcaat attttcaaaa
      601 ctgtttaaat ttattatctg ataagaactg gaatcagttt tattctgaat tatttgagtg
      661 tattttcaat ttggtggaaa cggaattttt gttaggcaat tatcaatctg caatcaagta
      721 ttttgagatt ggaatggaca atgcaaattc tagacttgat aaagccaagt tatacaactt
      781 acggattgct ctttttaata ctcagggaga ttatcaaaaa gcgattgaga gcggactcga
      841 tgggcttcgt ctctatgatt tcaaaattcc aagtaaaata aatgatttag ttttattcaa
      901 agaatatttg aaagtaaaga atgtgttggg tgaaaatcca attctattta tccaaagtct
      961 ttctgtttca gacaatcaag aagaattgga gaagataaaa ctttcgctaa atattttgga
     1021 ttctatttac ttaaccaatc catcccttgg aacatttata acaatcaacg caatgctttc
     1081 agtcatcaag attggaaatt cctacatatc gccgatcgtc tatgcatcct ttgcagcttt
     1141 tttatgcaat ataaatgaat acaaattggg ttataactta ggaaaatatg ctcttgaaaa
     1201 tatttctgaa agtttaaaag caaaatgcta tcatatcttt tgtagtttga tcaatcattg
     1261 gtctagatca gtaagagata cttttccatt gcaatcaaaa ggatttcaaa ttggtataga
     1321 aacgggcgat gtaatttatg cttgctataa tgcatttatg tatatttgga atcatttctt
     1381 tgcaggagta aagttagacg caacattaaa agaaggaaat aaatatttta aagcagtaga
     1441 aaaattaaag aatagagatt ctgaaaaatt cattttaata cttttaaatc atttgcgaga
     1501 gatcacagaa actagaatgg attattccga ttcagaaatt ttggagatgt gtgaggtgag
     1561 tcaaacaaag acaccttatt ttattttcta tttactgaaa tttcaaaaat attttattac
     1621 agaacagtat gaaaatgcag cgaaaatgat tccagaaatt ctagcaattc aggattcagt
     1681 cacaagcaca atgtatctaa ctgaatttcg gttttattac tgtttgaatc tattcataaa
     1741 agatgacaaa tcagaggaag caaacaagca aattgaaaaa gagctgaaac aatgggagct
     1801 ttgggcaaaa aactgtgagc caaattacca acataaatat tttcttttac aagcagaatg
     1861 gtgcaaggca aatgcaaaaa ttcaagaggc tatggagtat tatgataaag caatttattc
     1921 atctaaagaa tacgatttta ttcagaattc ggctcttgct tcaaagttag ccggtatgtt
     1981 ctatctctct ttggatagag gtaaaattgc agatttgtat ttttcagagg catttcaaaa
     2041 atataaaaac tggggaagtt tatattattc aaattttata aaaagtaggt atttaaaaaa
     2101 cttctatgaa ccttcctcat ctgacgttat tagccaaaag gtaagaggga caacttcctc
     2161 ttcagtcaat tcagtttata tagatattaa ttcaatccta aaagcttcta gaattatttc
     2221 gagtgacgtt cgatttgatt ccttatctaa aaactttcta caagtaatca tagaaaatgc
     2281 aggagcagaa agaggtgtat tgttaatttc tgaatccaat gagtttttca tttattcaga
     2341 agcaaatgta aatggcgaat ttaacttaaa caaaagaaaa caggattcta gtattctgcc
     2401 tacatctgtt ttaagatatg tagaattaac caaagagtca gttgtattac ataacgcatc
     2461 aaatgaaaag agattccaac gagatgaatt tataatagag aaaaaagtaa agtcaatggt
     2521 ttgccttcca attatgaatc aaaaccaaat gcttgcaatt ctttacttag aaaacaattt
     2581 atttgagaga gtttttaccg aagaacggat tgaaacgatt caaatgttat cttcccaggc
     2641 ggctgtatcc attcagaatt ctattttgta tcaaagtatg gaggaaaagg tcaaagaaag
     2701 aaccaacgaa ttgaagacca tgcttgataa agtaaatgaa ttaaagagac agcaagatgc
     2761 agactattat ttaacctcct tattaattca gcctctcagc aataataatt taaacagtag
     2821 aatcaaaatt gagttcctag tcttacaaaa aaaagagttc gaattccgaa aagaaagtaa
     2881 ttttattgga ggagatattt gtatatccga tgaaattgtt ctacgaaaga agaaaatgat
     2941 tgtctttgtt aatgcagatg caatgggtaa gtcattgcag ggggcaggtg gaactattgt
     3001 tttaggttct gtttttaaat ctatcttaga acgcactaaa atggacgata gactccaaag
     3061 tcagtttccg gagctttggt tgaaaaatgc gtttagtgag ttacataaag tctttcaaac
     3121 ttttgaaggt tttatgttgg tatctgcgat cattggattg attgaagaag aaactggctt
     3181 tatgtatttt ataaattctg aacatccgca cagtgttttg tatagagatg gaaaggcttc
     3241 ctttattcca aagacccaaa gtctttttaa aatgggatcg ttaggtggtg atgatagtat
     3301 tgtaattatt cagacatttc agacatatcc aggtgatata attataaatg gttcagatgg
     3361 aagagaagat ttaattaaag attatacaga gcatggtaaa ccaaatgtat accatgaaga
     3421 aaaattattt ttagattatg tagaagaagc gaaaggggat ttacacacaa tcaaaaatct
     3481 aatcgtaaat tcttgggagc tgtatgatga tttatcactc ttaagggtag aaatcccaga
     3541 gaacttggaa atccaaaata tagagaaaaa aactcttttt gattttattc ctgattatct
     3601 ttcgttggat aagaatacaa aaaggattca actactagag ctatacaatc aaaatttaaa
     3661 agagaacaag aatctctatt tatataaatt attatccaaa gaatttgtga aattcagaga
     3721 ttatgaaatc gcagccaatt gtgcaagaat ctattgtgaa tcgaatccat cggatcttag
     3781 aatgttaaaa ttctatgctt ttatattaga aaagattgga gagtttgacg aggcagatct
     3841 ctataagcag cgacttctac ttagagctca tacatattca caatccaggc tttaaaggga
     3901 cgactgacgt aaaaatcttt ttctatcata cgattttaac gatctaaatt ctattttttt
     3961 gcaaaatacg ataactctaa aactatcaaa actgcaataa aatctttgac attccatttg
     4021 ttttctatag aataataaga atctaaaatg gaaagattct ccaatggaga gccgttattc
     4081 tatagaggtt tattgtaagt catatcgaat gctatctgag taaagtcttt atggagaatt
     4141 acttttctca tttggtattt ataaaaataa aaatgaatat tatttttaga ataattttat
     4201 atacaagtat tcttcttatc gtattttcct gtttgaaaac gaaaagatct ctttttgatt
     4261 ttagttctcc tgctgggatt ggaactttct ttgtttttag caatgttcct tccaatcaac
     4321 ctacaaactc caatacaaca acggaaactg tctcctctcc tagttttact ccgcaagcgg
     4381 gaacttataa ttcaatcata gacattgaaa ttaaaactac aacagctgat gctgtgattt
     4441 attacacttt ggatgggaca gatccaactc ttgcttctaa taagtataca agcccaatat
     4501 cgattcgaac gatagctggc ttgactctaa aggcaattgg aatcaaatca aatttttcga
     4561 atagttctgt gactgctggt atttttagtc ttctgcctct caaaacaggt cagactactt
     4621 ctttttcgag tggagatgat ggaaataaaa atcttggaac actaagaagt ttttcaggtc
     4681 ctacccctca tagtgtctat acctccgatt ttacaacgac agacaatatt actggacttg
     4741 tttggaagac ttgcgcagag gggtctacag ggagtaattg tggtggaggg tctttgcaat
     4801 cgaatacttg gtcttctgcg cctagctatt gcacaagttt gaattcaaat aatagtaata
     4861 acggttttgc gggaagaacg aactggagaa ttactagtaa acaagaatta gagtcaatca
     4921 ttcattatgg agcttctaat gaagcgatat acgcaacaaa ttttcctaca aacgggcaac
     4981 ttcttggtcc tatatgggcg agtgaagaat acgatattag cactgcttgg atcattgatg
     5041 aatcaaatgg ttattctttt aatttagcaa aaggaaactc agctgttttt atccgatgtg
     5101 tatcaggaac agcttataaa tattctgcca ttttatctga taataataat ggaacgattc
     5161 aagataaaag aacaggactt atttggcaaa aatgtagtcg gggacaagat cctgctaatt
     5221 cttgctcggg aggtattaca tcagatactt ggactaacgc attgtcttat tgcaattctc
     5281 tcacacttgc ctctaagtct tggagacttc cgaatataaa tgaattaaaa acgatttcga
     5341 attttgaagc aactgctccc aaaatagatt cctcattttt tccttcgaca gctagttctc
     5401 agtattggtc ttctacaacc tattttcctg gaactagttc tgctttggtt ttggattttg
     5461 gaactggtgg aattatctac agccctaaat cgaattcatt tgcgattcga tgcgtatctg
     5521 gacaataata tttttgagta tttagtttag actagaatcc aaaagatagt ctactacgat
     5581 aagcgcaata tcatcctcta acttgtcttc cccgggagac caatcgataa ctttttttag
     5641 taagctttct ccaaattctt ttgcgctcat atttgccgat aattttaata gttcttgaat
     5701 cttctcctca ccaaaaagct cacctgtatg atttcctgct tcaaagagtc catctgtata
     5761 gagtaaaatt ctatctccaa aaagtaaatg aatttccgtt gaatcgaaga gtgaatcttg
     5821 cgttaggcta agagctctcc cctttggttt tagtgagatt aattcacccg tttgctttct
     5881 aaaaatccag agagcgctat gtccagcatt tccagtaatt agaattttct tttccatgtc
     5941 gatataggca tagcaggcag ttacaaattc accacctgtt ttatcacgta gtatttcatt
     6001 catgcttttg agtaggtcag aaggatactc tatgtttaat tcttgaaatc cgaaggcggt
     6061 tttaacagct gagacaacaa gtgctgcagg aactccatga ccagatacgt cagccattat
     6121 aaaaccaagg ttactttctt tggttctaaa gtcatagaag tcgccgccta ccgtttccat
     6181 gggacgatac caagctgcaa tttcgagccc aggtatattt ggaattcttc tgggaatgat
     6241 agattgttgt atttttctag ctaactctaa ttctactttt aatcgttctg atttttttag
     6301 agcagagata gattctcttt gggctttttc tttttcttgt tctaaaattt taattcgatc
     6361 tgcaagtgca aaggaaatta aaatcgattc aagacctagt ccaaactgtg ctaaatttcg
     6421 ggtgaataca ttgataggaa ctattccaaa accagtgagt gctagaagca aagcaccgag
     6481 taataatatt ccccaagcta gtaaatagat tttggctgga cgatagcctt cttttgctaa
     6541 taaaaatcca gccgctattg ccgctaaaga atttgcgata acaaagaaga ctaacgttct
     6601 gctaatcaaa tcatatcgga caaaccaact tcctataaat gcaagagcag atatataaat
     6661 gagcaattta ataaatcgat ccattttagg atgattgatt ttaagtttga gaaaggatct
     6721 tacaaataat aaaatgccta aattatttag agcaagtaga acaggcgcaa aatcacgatt
     6781 aaaccaaggg ttgtctcccc ataaaaattg agccgtgatt ccattgtaac ccattaaaaa
     6841 taaactagca ggcagagtaa aagataaata gaaaaaataa actttgtctt tgagtgcaat
     6901 tcctaatagt aaattgtata aaaacatgga gaacattaca cctgcaagaa ccaactggaa
     6961 ggcaaaatta tattggtctt tctcaaagaa aagtttttgg ttgtgaatga aaacgggcat
     7021 ttggatagaa ctttgtgttt tcactcgaat aaaaaatctt ccttttggtt tgatatctct
     7081 gagagagact aggtaggttc tgtgtttaaa atctctctca gaaaaaggat ggagtctacc
     7141 gagtaacttt cgaaccagtt gattcttact atcagtaaag aagaattcta tctcatctag
     7201 atttgcatag gctacttcta aaataaataa atctaaagaa gtt
//
LOCUS       DAOQOS010000197         7057 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_6019_length_7057_cov_5.960297, whole genome shotgun sequence.
ACCESSION   DAOQOS010000197 DAOQOS010000000
VERSION     DAOQOS010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 7057)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7057)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7057
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6019_length_7057_cov_5.960297"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            313..2001
                     /locus_tag="PLF66_24680"
     CDS             313..2001
                     /locus_tag="PLF66_24680"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HMZ67306.1"
                     /translation="MIKKYIILLFISVSLVQCLEVKKSPFDLSSPNLFTIGLFIFLNN
                     SSTSGDRTAPTLTVTNLKSKMTIEFGAILGTASDNVSVSKVEVQLNNGNYSLATGTTS
                     WSYKLPLTGWLTGNQNTINVRVTDSSGNTTINPYTLLTKGQNKDVNGDGYLDLIVSAP
                     NYNSNQGRVYVFHGSASGISSATASSASKIYTGFNVNLFGKNLALGDFNQDGYSDLFI
                     CSSLNGNANNRGDLYLGSATGLANTATTYLPPSGTNTEYCSSVQPGDFNGDGYTDLVV
                     SDIGYSNPPNNGYLYIYYGSQNGLSTSVGTTLVSGAGSNLGIGMSVGDINGDGYADIV
                     ATNSGSATVYLFFGAAAGVSTLTTAFNGTGVSASGFPYLMDINGDYKADFLSSNTSFN
                     SNVGRVGVFQSTGASFNSTPVFTIDGDGPNYLFANFITSADTNGDGTLDIVFGNPSGN
                     HPNGNQGAVYVIQCAVGATCAGSGTASSLASLKITGGATQDFFGSFVTFRDVNRDGYL
                     DLIAAARGYNSGTNQGALYIFNGTATAFSNSTVSANSKMITGESAANSFGNSGF"
     gene            2276..2953
                     /locus_tag="PLF66_24685"
     CDS             2276..2953
                     /locus_tag="PLF66_24685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67307.1"
                     /translation="MLSRLFIIVYVFVASVVFAQSPGSSSNTSRPQGAQKSEQGDSVS
                     GAPAKGNYANGLSQNIVTLQKSINDKVDKLNKLQVQLSEVNKSLGSHVTIPEEEPNIK
                     KEEGVIVVSARRIEFTFQGNNTSEVKIISSKRRINNDLHSVVRTLTFNPGDFNSIKVK
                     VDQFDSKMKGVPFEESYNAMSPDVQLVALKAIDLVLFNTIYRYDNYLQKRTIDKQENT
                     KSHINEL"
     gene            3423..6458
                     /locus_tag="PLF66_24690"
     CDS             3423..6458
                     /locus_tag="PLF66_24690"
                     /inference="COORDINATES: protein motif:HMM:NF021606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HMZ67308.1"
                     /translation="MADKESIEKLAKRIDALENELISVKSELNKLSQGEAPIQIARPE
                     YKPNIIPPKLQEVEAKPKKSFDWEILLGGNILGKLGLAAIILAFIWFLNFAFDNHWIN
                     ESGRIFIGEIAGFAIIGIGIILAKRKMQIVPEPVIGTGVSIVYLSLYGGYYYYDLLKA
                     SETFIAFTVLSLGTSLLAGRANLQVLYIFSLLGSILAPVLLSSGENSYRFLFIYLTLV
                     NGVFYFISQKNTWRISPYLVFLANVVIFSQWGESKLAQSSFLPPFLFLIILFLLFGMR
                     EVYLMPRIRKAVDVSSSVLLLLIILSFSGLGYWVVNVFYSNWTPHFLLFSSFLLVGFL
                     KILERYSLPLLDTNLRYGKSLSAVLFLSWIAVLFLAVSNFSRGQWITVSWILFAGGLS
                     AIGAYFQSRTYLLISVIPWILALFRLYFVESFHDRQFYFLLNTRFGLFVLATVFLLFT
                     YQVQKKIQIHKSVVGFVFVALFTLILGTLVEVRYLVSDTYYRNLGYSYVIAFYMIALL
                     VPGFKFSYRSFRLTGIILGVILIAKFYLYDIWMLSIVVRIIAGFSLGVGLVILSIIYQ
                     KFKDKINVNQILKLTAFPLLVFLFFSSKNLTAEPFKNNGYKYFTEIKGLNKEVVDDSN
                     NIYGRFKLTEEISRFHGSSDLRLVYNNELVPFIHRRVTQVAGKSGKTTPTVIYTNVTT
                     NGRIYVLKFEEPPAKTEYTELEIAGSEKYETGVYMSLGDEPNQWEETVSAAIYNYYDE
                     QQANKINRIKFRSGKYRYARIELDSKIQLDFTGAIYSPLKEKAEYKLEISKNDFSISE
                     DSDRKASVYTFENPMRKPISRIVVNFEENKYQRSIEILQKDIISKEFTIMADSVIYRK
                     PDTNGEHSIELPYYTGGNLKILVVNNEDKPLTLKSIDAYSEIEEIVFEIPKNLNDTEP
                     ASLLLYYGNEYVRFPEFDLKNTYDEKLKHVSLKAGEHKNNPEFKYTLVEPPMSSWIIR
                     GLFLIGVLLMTYPAFTILKKYRTELDLELPREASEKEEN"
     gene            complement(6712..>7057)
                     /locus_tag="PLF66_24695"
     CDS             complement(6712..>7057)
                     /locus_tag="PLF66_24695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002974003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1499 domain-containing protein"
                     /protein_id="HMZ67309.1"
                     /translation="LPCPATPNCVSSQSDPTDNEHYILPYKYSVDTNLAFTQLKTLLE
                     KQDRVKIINSNDTYINAEFTSLLMRFVDDVEFYFDEKEKMVHIRSASRLGKGDMGVNR
                     KRMELIRKELGW"
BASE COUNT         2300 a         1053 c         1305 g         2399 t
ORIGIN      
        1 aatctcatta tttgctaaaa taggaagtga gaataataaa aacaagcgta agaagatttt
       61 aatcattttg gtttcactct caacttggca tttaattttg taaatagatt aaactcatac
      121 tatagaccga gtatttagga tatttcttct aaggatttca tcaaaaatcc gtttgaaatc
      181 attaatttat gtttccccaa gttttaaaaa taattgattc tttttgaaaa gcaattttat
      241 tctatttgtc ttaataagta tggaactaga ttttgatatt aaaaatttta ccgttcccaa
      301 gtatagaatt ctatgatcaa aaaatatata atcctgctat ttatttcggt gagtttggtt
      361 caatgtttag aagtaaagaa atcacctttt gatctttcat ctcccaatct ttttacgatt
      421 ggacttttta tttttttaaa taatagttct acatctgggg atagaactgc tcctaccttg
      481 acagtgacaa atttaaaatc taaaatgaca attgaattcg gagcgatttt agggaccgct
      541 agtgataatg taagtgtctc taaagtagag gtccagttga ataacggtaa ttattcgtta
      601 gccacaggaa caacatcttg gtcttataaa cttcccttaa caggttggct cacaggaaat
      661 caaaacacaa taaatgtccg tgttactgat agtagtggaa acacaaccat caatccatac
      721 acactactta caaagggtca gaataaagat gtaaacgggg atggttattt ggatttaatc
      781 gtttctgctc ctaattataa ttcgaaccaa ggaagagtgt atgtatttca tggaagcgca
      841 agtggtatta gtagtgccac tgcaagttct gcgagtaaaa tttatacagg tttcaatgtg
      901 aatttgtttg ggaaaaatct tgctcttggg gattttaatc aagatggtta tagtgatttg
      961 tttatttgct cttctttgaa tggaaatgca aacaatagag gggatttata cctaggttca
     1021 gcaaccggtt tagcaaatac cgcaacaacc tacttaccac caagtgggac aaacacagaa
     1081 tattgttcta gcgtgcagcc gggtgatttc aatggagatg gatatacaga tttagttgtt
     1141 agtgatattg gttattcgaa tccacctaac aatggatatt tatatattta ttatggaagc
     1201 caaaatggat tatctactag tgtgggcaca acgcttgttt cgggtgctgg ctcaaatctt
     1261 ggaattggaa tgagtgttgg agatataaat ggagatggct atgcggatat cgtagcgacg
     1321 aatagcggtt ctgcaactgt ttatcttttt ttcggggcag cagcaggagt ctctacgttg
     1381 actaccgctt ttaatgggac aggtgtttcc gcttctgggt ttccttattt aatggatata
     1441 aatggagact ataaagccga ttttctttct tcgaatactt cttttaatag caatgtgggt
     1501 agagtaggtg tgtttcagag cactggagct agttttaatt caacgcccgt atttacaatt
     1561 gatggagatg gacctaatta tttgtttgcg aattttataa catctgcaga tacgaatgga
     1621 gatggaacgt tagacattgt atttggaaat ccaagtggga atcatccaaa tggaaatcaa
     1681 ggagctgtct atgttatcca atgtgcggta ggtgctacat gtgcaggaag tggaacagca
     1741 tcttcactcg caagtttaaa aattacaggt ggtgctacac aagacttttt tggaagcttt
     1801 gtaactttta gagatgtaaa tagagatggg tatttagatt taattgcagc tgcgagaggt
     1861 tataactcag gaacaaacca aggtgctttg tatattttca acggaactgc aactgctttt
     1921 tcgaattcga ctgtaagtgc aaatagtaaa atgataacag gtgagtcagc tgctaattca
     1981 tttggaaata gcggttttta gttttgttct agatttctga gtttataatc tttcttgagt
     2041 attctaattg atttgtaaat gcaaaatgca aagaatgttt taaatacttt ttgtatttcc
     2101 tttcacctag ttatttaatc acttgatgat agcctaagtt caataaaaat ggcatatagg
     2161 aaagggattt tccttggaaa atttataaat atttggaaat aaattatttc ccggaaagtc
     2221 gtatcctaaa aaattaaaaa tataaatgtg attttagctt ataggaggta gcgaaatgct
     2281 atctagatta tttatcattg tgtatgtgtt tgttgcaagt gttgtttttg cacaaagtcc
     2341 aggaagtagt tctaacacaa gtagaccaca aggcgctcag aaatcagagc aaggggattc
     2401 cgtctccggc gcaccagcaa aaggcaatta tgcaaatggt cttagccaga atattgtaac
     2461 tttgcagaag agtatcaatg acaaggtgga taagttaaat aagctacagg tgcaattgtc
     2521 ggaagtaaat aaatctctcg gttctcatgt aacaatccca gaggaagaac caaatattaa
     2581 aaaagaagaa ggcgtgattg ttgtttctgc tagacgtatt gagtttactt tccaagggaa
     2641 caatacttca gaagtaaaaa ttatttcaag caagagaaga atcaataatg atttacattc
     2701 tgtagtaaga acactaacgt ttaatccagg agatttcaat agcatcaaag tgaaagtaga
     2761 tcagtttgat agtaaaatga aaggtgttcc ttttgaggaa tcttataatg ctatgtcgcc
     2821 tgatgtccaa ttagttgcct tgaaagcgat agacttagtt ttatttaata caatctatcg
     2881 atatgacaat tatttacaga agagaacgat cgataaacaa gagaatacca agtctcatat
     2941 taatgaactt taaaacagaa agattctaaa aacaaatttt acaaatttgg aaaaagctat
     3001 tggcggttct gctaatagct ttttttattt cctttacatt ttcaatcttt tcaagaaacc
     3061 cagtagattc tagatttatt gcccacaata ctctccaaat tcgagatcgg atgaaaacta
     3121 aagcgaaaca ttgattttaa aaattttttc ctaaattcta tctaaataaa ttcttggagg
     3181 gaggtgggaa tttcccttat tttttaaatt ctatttcaat ccgttctaca agcttgaaca
     3241 aatggactgc attgttaagt gtgaaaaaaa tttttttctt ccattgaatt caaattttgc
     3301 aattcttagc gactcatcat tggttttaaa atggatagat ttctattttc ctcttcgatt
     3361 gaaagttgat ggttcggaaa aattcttgct caaaaaaaaa gaaacgctaa aactgtccat
     3421 ctatggcaga taaagagagt atcgaaaagt tagcaaagcg tattgatgca ttagaaaatg
     3481 aattaatttc tgtaaaatca gagttaaata aactatccca aggggaagct cctatacaaa
     3541 tagcgagacc agaatataaa cctaacatca tccctccgaa attacaagaa gtagaagcaa
     3601 aacctaaaaa gtcctttgat tgggaaattt tactaggtgg taatattcta ggtaaattgg
     3661 ggttagccgc aattatttta gcctttattt ggttcttaaa ttttgctttt gataaccatt
     3721 ggattaatga atcaggaaga atttttatcg gagaaattgc aggattcgca atcatcggga
     3781 ttggtataat attagctaaa agaaaaatgc agattgtgcc tgaacctgtg atcggcacag
     3841 gagtatcgat tgtttattta agtttatatg gtggctatta ttactatgat cttttaaaag
     3901 cgagtgagac atttattgct tttacggtat tatcattggg cacatccttg ttagccggta
     3961 gagccaattt acaagttctg tatattttta gtctacttgg atctatactc gcacctgtgt
     4021 tactttcatc gggtgaaaac tcctatcgat tcttgtttat ctatctaact cttgtgaatg
     4081 gcgtattcta ttttatcagt caaaaaaata cttggagaat ttctccttat ttagtatttc
     4141 tagcgaacgt cgttatattt tcgcaatggg gagaatccaa gctcgctcaa agtagtttct
     4201 tgcctccttt cttatttttg ataattttat ttttgctatt tggaatgaga gaagtctatc
     4261 taatgccaag gattcgaaag gctgtagatg tttcgagtag cgttttattg ttactcatta
     4321 ttctgtcatt ttctgggctt ggatactggg tagtgaatgt tttttattca aattggactc
     4381 ctcacttttt attattttcc tcttttctac ttgtgggatt tttaaaaatt ctagaaagat
     4441 attcacttcc acttttggat acaaatcttc gatatggaaa atctttatct gctgttttat
     4501 ttctatcttg gattgcagtt ttatttttag cagtatcaaa tttttctaga ggacaatgga
     4561 ttacagtttc ttggatctta ttcgcgggtg gtttatctgc tattggcgca tactttcaaa
     4621 gcagaactta tttactaata tctgttattc cttggatatt ggcactgttt cgactctact
     4681 ttgtagaaag tttccatgat agacaattct atttcctact aaatacaaga ttcggattat
     4741 ttgtattagc cactgtattt ctattattta cttatcaagt tcaaaagaaa attcaaattc
     4801 ataaatcagt tgtaggtttt gtatttgttg cattatttac tctgatctta ggaactttgg
     4861 ttgaggttcg gtatttggtt tcagatactt attatcgcaa tctaggttac tcctatgtaa
     4921 ttgccttcta tatgatagca cttctagttc ctggatttaa attttcttac aggtcttttc
     4981 gtttaacggg tattatcctc ggagtgattc taatcgccaa attctatctc tatgatattt
     5041 ggatgttgag tatagtagtt cgtattatag ctggcttctc gctaggagtc ggtctggtca
     5101 ttttaagcat tatctatcaa aaatttaagg ataaaatcaa cgtgaatcaa attttaaaat
     5161 taacagcttt ccctttacta gtgtttctgt ttttttcttc taaaaactta accgctgaac
     5221 cgtttaagaa caatggatat aaatacttta ctgaaataaa aggtttaaac aaagaagtag
     5281 tcgatgattc aaataatata tatggtcgct ttaagttgac tgaagaaatc tccagatttc
     5341 atggaagttc agatctgaga ctggtctata ataatgaatt ggttcctttt attcatagaa
     5401 gagttacaca ggtagccggt aaaagtggta aaactacacc aactgttatt tatacaaatg
     5461 tcacaacgaa cggaagaatc tatgtattaa aattcgaaga gccgcctgca aaaacagaat
     5521 acacagaact agaaatagct ggatcagaaa aatatgaaac aggtgtctat atgtcacttg
     5581 gcgatgaacc aaaccaatgg gaagaaaccg ttagtgcagc tatctacaac tattatgatg
     5641 aacaacaagc aaataaaatc aataggatta aatttcgttc agggaaatac cgttatgctc
     5701 gtattgaatt ggattcaaag attcagctag attttacagg tgcaatttat tctccgctga
     5761 aagaaaaagc agaatacaaa ttagagattt ctaaaaatga tttttcaatt tcagaagatt
     5821 cagatagaaa agcatccgtt tatacttttg aaaatccgat gcgaaaacca attagcagaa
     5881 ttgttgtaaa ttttgaagaa aataaatacc aaagaagtat agagatatta cagaaggata
     5941 tcatttcaaa agaatttaca atcatggctg attctgtaat ttatcgaaag ccagatacaa
     6001 atggggaaca ttctattgag ctaccgtatt atactggagg taatcttaaa atattagttg
     6061 tgaataatga ggataaacct cttactttaa agtcgataga tgcctattct gaaattgagg
     6121 aaattgtttt tgagattcca aagaatttaa atgacacaga gccagctagt ttacttttat
     6181 attatggaaa tgaatatgta agattccctg agtttgattt aaaaaatact tatgatgaaa
     6241 agttaaaaca tgtaagttta aaagcaggtg aacacaaaaa taatccagaa tttaagtata
     6301 cgttagttga accgccaatg tcttcatgga ttattagagg attatttttg attggagttc
     6361 ttctgatgac ttaccctgca ttcacaattc taaagaaata tagaacagaa ttggacttgg
     6421 aattaccacg agaagcaagt gagaaagaag aaaactagct gcaaagacgg gcaaagattt
     6481 taaccacgga tatgtaatga tagataatgt gagtttgatc tagaaaaaaa ttaaaagatt
     6541 accaccgata aacaccgagg acaccgataa aaatatcgaa ttcaggttaa attgggacaa
     6601 atggcttcga taaatattag atctatgtcg tcctatcgtt cagttatcgg tgtaaatcgg
     6661 tggtggtcag ttctcttttt acttctggaa tagactggcg attgaaaaaa tttaccagcc
     6721 tagttctttt cgaatcaatt ccattcgctt acgattgact cccatatctc cttttcctag
     6781 tctagatgct gatctgatat ggaccatttt ctctttttca tcaaaataaa attctacatc
     6841 atctacaaat cgcatgagta gagaagtaaa ttctgcattg atataagtat catttgaatt
     6901 tatgattttc actctatctt gtttttccag aagagtttta agttgtgtaa aagcaagatt
     6961 tgtatctact gagtatttat agggaagaat ataatgctca ttatccgttg gatcggattg
     7021 actagataca caattcggtg ttgctggaca gggaagg
//
LOCUS       DAOQOS010000198         7044 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_6043_length_7044_cov_6.073115, whole genome shotgun sequence.
ACCESSION   DAOQOS010000198 DAOQOS010000000
VERSION     DAOQOS010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 7044)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7044)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7044
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6043_length_7044_cov_6.073115"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1379
                     /locus_tag="PLF66_24700"
     CDS             <1..1379
                     /locus_tag="PLF66_24700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67310.1"
                     /translation="LYVLGLFISAIFVFILYRKVFCKKLLKYSLITFIINLLITNIFL
                     LISIPLVFYSSSINMGGAASYSIKQILLTWTGLVDFGYWVSDIDINLNSVSMSIGNFI
                     LLPLMVITVILFVYNYKKKREVLSYILLIIFLFGMSFYYNQRGLSYQAVRFTEIGHGY
                     LLGLVGLGLVSNKKDTKVLSALSGMLYFLILIYFLNVRIDTSKEILQPKKNTHSEYRN
                     HSEFELVDKLAEIQKKYSREEGLIYYSGNGFGVELAGISVLFRNLRYVSSFGFDYGIL
                     VGVDNSKEEITLWDKKLLNKALIVELNTKNEIIEDNRKGVNKDFIFTNDKYRIVKTEG
                     VQYSSLVGAYWGPLVQTDINPNFPNFRYLKNEESSLVIWANRNEKVKIEFTVYADREG
                     GKVLVKADNKNYNFYLKKWNGNFSEIETISILKELKPGPNVITLKPYSDDNIVTWLIL
                     WKVTINSI"
     gene            1543..2355
                     /locus_tag="PLF66_24705"
     CDS             1543..2355
                     /locus_tag="PLF66_24705"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HMZ67311.1"
                     /translation="MTQLSIITINLNNKEGLQKTIDSVLNQTFRNFEFIVIDGGSVDG
                     SLELIDKYKNEFAYWISEKDNGIYDAQNKGIVKAKGEYCLFLNSGDFLIQNDVLQKVF
                     ERGFETDIIYGDMLIDYGKDTVLYGKSPDKLTLYFLAYEVLWHCVTLIRRNLFDRYGL
                     YDSSYKIVADVDFFLKAIGVGKASYSYINLPISQFNTAGFGSDPKNANLLEKERIQSR
                     ETVLSPLILETLKENREMKSELHIYRNSKSLKLINRIRENWFVSQILSLLKR"
     gene            2360..3739
                     /locus_tag="PLF66_24710"
     CDS             2360..3739
                     /locus_tag="PLF66_24710"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HMZ67312.1"
                     /translation="MISIIVLSWNRELLLRNTILSLLATTKSEFELFIVDNASTDGSK
                     EWLQVFSTYHKNVKVILLEENLGGEAFNIPLSEARGKYILFSENDLEYLPGWDEYMIK
                     QFEKFTDLGQLSPFAPNPMREIGEVWIDKPFEIVKRQEDILLKAIHNVTTTCMIRSEL
                     ISKGLKWVNLLSKDGLFKFPADASFSLDVKNMGYLVGWSNRYMALNWGFNERVIAKEK
                     EYYEKNWKEKSNLKIDGISLDKTESRETNDSVSQQTIAKLQIENYDLRQKIFTLGGES
                     IQLYFDTGNGFSESDSEKVLIEQDKKKYEIPLDESKNYLQFRLDISNNPIKVMLNSIY
                     IIDRNNSEYKLKIKESNGVLVNEREYIFSNFDPQFYLEPFSKPIQIKKFVLECSQIES
                     NVLVYQTALQISNQAKESIVEEKNKRIESILNSKPYILGSLIVKPFDINSWKKLFHSI
                     KKILSQFRN"
     gene            3743..4591
                     /locus_tag="PLF66_24715"
     CDS             3743..4591
                     /locus_tag="PLF66_24715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67313.1"
                     /translation="MEEKMDIKVRQIFFKKEQINKVSDNCFPYFNSSDFESNLFLEYS
                     VILKSYLDGFYKEGDLAGVLSWRFFEKARKTESEFFDFVRKNPGYDVYFINPFMEQVT
                     MYESVWHQGGTNHPGLIEVADRIFQDLNYGFKTSDVKNTTADTLYCNYWIGTPKFWEK
                     YIAFTKPIHDYILENKNDPETIKKLKSNAPYHYPVSYIPFLMERLFSTLLWKDSTIKY
                     LGYQYSPLEIAYLINELRGYEYAYKKIDVWYNLFHQNKIILFLKHIRNFLLRVKAFIK
                     RNTNAD"
     gene            4581..5519
                     /locus_tag="PLF66_24720"
     CDS             4581..5519
                     /locus_tag="PLF66_24720"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HMZ67314.1"
                     /translation="MQTKESEKVNIGIIGSTGFLGRSLTNYLSQKYSVHVYSRREPSD
                     LFENISSWTIGQLHDEKVLQGFLERIEILIHLGQSGKPMESNNNFVYETEANLIPSLQ
                     LIEVLKKTKNSIHILFASSGGTVYQENPDRFYLETDPTEPLTSYGINKLSLEYYFNLL
                     TKISSHSCFTLRISNPYGELLSSGRNQGFIGVSLSRILEGKEVLIFDDENMVRDYIHL
                     EDMCSAFEQAIQFRKAGYSTFNIGTGVGTSLREILVIMEKYFKKTIPRTIVKKDSMIK
                     LPRWSVLNIQKAREELNWKPQIELDSGLQKLIESIQ"
     gene            5516..6541
                     /locus_tag="PLF66_24725"
     CDS             5516..6541
                     /locus_tag="PLF66_24725"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HMZ67315.1"
                     /translation="MKALPKVSIIIPVKNPGKILERVLSSIYDQSYPSSFEIIIIDSG
                     SVDGTLEFLNESKKNNPILKVYSIPSSEFGHGKTRNLAIQKSSGELVAVLTHDATPVN
                     QYWLSNLVQPFLENDSVAGVFGRHYAYEDADIFTKRELNQHFDGFNRTPPITYYKIED
                     FDSYYKNKNLQMFYCFFSDNNAMLRKSVWEKIPYEDVNFAEDQKWAKAILENGYTKAY
                     ADQAIVYHSHNYTITEVFRRSFEEAVSFQLHFQHRVIESILTLVKIILIRTRIEYSVT
                     IRSRDLSFFKKIYLLFNRPIYNLFRFGGYYLGSNRKISNLLYNRVSLDHKLFTEGKGR
                     SKDKNND"
     gene            6534..>7044
                     /locus_tag="PLF66_24730"
     CDS             6534..>7044
                     /locus_tag="PLF66_24730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67316.1"
                     /translation="MTRIIQIIQYIYRLIAKKIKVLLRDFILYIVSPKDDLVSKVYYI
                     FIASKYGTPYTLNKNELDVNWFIPAFNPGSGSGGHLNIFRMVHFLEKYRIKNNLVIVE
                     SNHLTKKQSKKIINEDYIPVQAELYEGRSEAKPALISMATEWRTAYPVRDFIGSPIKT
                     YFVQDYEPLF"
BASE COUNT         2577 a          914 c         1259 g         2294 t
ORIGIN      
        1 atttatatgt tttaggtctc tttatatcag caatttttgt atttatttta tataggaaag
       61 ttttttgtaa gaagcttctt aaatactcat taatcacatt cataataaat ttattaatta
      121 cgaatatttt tctactaatt agtattccat tggtatttta ttcctctagc attaatatgg
      181 ggggagctgc cagctattcg ataaaacaaa tactacttac atggactggc ttagtagatt
      241 ttggatattg ggtatcagat atagatatta atcttaattc tgtaagtatg agtattggta
      301 attttatatt attaccatta atggttatta ctgtaattct atttgtttat aactataaaa
      361 agaaaagaga agttttatct tacatactac tgataatttt tctatttggg atgagttttt
      421 attataacca gagaggtcta tcctatcagg cagttaggtt tacagaaata ggtcatggtt
      481 atttattggg tttagtaggt ttaggattag tttctaataa aaaagatacc aaagttctta
      541 gcgctctcag tgggatgctt tattttttaa tactaatcta ttttttgaat gtaagaatag
      601 atacatctaa ggaaatacta caaccaaaga aaaatactca tagtgaatat agaaaccatt
      661 ctgaatttga gcttgtcgat aagcttgctg agattcaaaa aaaatatagc agagaggagg
      721 gattgattta ttactcagga aatggatttg gtgttgaatt agctggaatt tcagttctat
      781 tccgaaatct aagatatgta tcaagttttg gtttcgatta tgggatattg gttggggtag
      841 ataatagtaa ggaagaaatt actctttggg ataaaaaatt attaaacaaa gcattgatcg
      901 ttgagttaaa cactaagaat gaaattatcg aagacaatcg aaaaggtgtt aataaggatt
      961 ttatttttac aaatgacaaa tatcgaatag taaaaactga aggtgttcaa tactcaagtc
     1021 ttgtgggagc atattggggt ccattggtac aaactgatat taatccaaac ttccctaatt
     1081 tcagatattt aaaaaatgaa gaaagtagtc tagtaatatg ggcgaatcga aatgaaaaag
     1141 ttaaaattga atttaccgta tatgctgata gagaaggagg aaaagtttta gttaaagctg
     1201 ataataagaa ttataatttt tatttaaaaa agtggaatgg aaacttttct gagatagaga
     1261 cgatctcaat tttaaaagaa ttaaagcctg gacctaatgt gattacttta aaaccatatt
     1321 cagatgataa tattgtaaca tggttgattc tttggaaagt aactattaat agtatctaac
     1381 ataaattcaa gtaaacttta atctcttaaa gcatttctcc tccaaagaga tttccttaaa
     1441 tccacaaaca acttaaagaa aattagaagt tcaggaaatt ttctgtagaa agactcctct
     1501 gttaatgaat actattcaat tctttcaatt ctaaaaacac aaatgactca attatcaatc
     1561 attacaatca atttaaacaa taaagaaggg cttcagaaaa cgatagattc cgttctaaat
     1621 caaacgttta gaaattttga attcattgtg attgatgggg gatctgtgga tggaagttta
     1681 gagttgatag ataagtataa aaatgaattc gcttattgga tcagtgaaaa agacaatggg
     1741 atttatgatg cgcaaaacaa aggcattgtg aaagcaaaag gggaatactg tttgttttta
     1801 aattcgggag attttttgat tcaaaacgat gtgttgcaaa aagtttttga gagaggattt
     1861 gaaacagata ttatttacgg agatatgtta atcgattacg ggaaggatac tgttttatat
     1921 ggaaagagtc cagataaatt gacgttatac tttctagcgt atgaagtttt gtggcattgt
     1981 gtcactttga tcagaagaaa tctttttgat cgttatggtc tttatgatag ttcctataaa
     2041 attgtagcgg atgtggattt ttttctaaaa gcgattggag ttgggaaggc aagttattct
     2101 tatatcaatc taccgatcag tcaatttaat acagcaggat ttggatctga tcccaaaaat
     2161 gcaaatcttc tagaaaaaga aagaattcaa agtagagaga ctgtattgtc tccactgata
     2221 ttagaaacat taaaagaaaa tagggaaatg aaaagcgaat tgcatattta tagaaattca
     2281 aaaagtctta aattgataaa tcgaattcga gagaattggt ttgtatctca aatattatcc
     2341 cttttaaaaa gataaaaaaa tgatcagtat aatagtttta agttggaatc gtgagttatt
     2401 acttcgaaat acaatcttgt ctctgcttgc gacaacaaaa tcagaatttg aattgtttat
     2461 agtggacaat gcatctactg acggttcaaa agaatggcta caagtttttt ctacctatca
     2521 taaaaatgta aaagttattt tactagaaga gaatttaggg ggagaggcat ttaatattcc
     2581 tctgagtgaa gcgagaggta aatatatatt attctcagaa aacgatcttg aatatttacc
     2641 tggctgggat gagtatatga tcaagcagtt tgagaagttt acagacctag gtcaattatc
     2701 tccttttgca ccaaatccaa tgagagaaat tggagaagtt tggatagaca aaccatttga
     2761 aattgtaaag agacaagaag atatattact gaaggcaatt cataatgtaa caacgacttg
     2821 tatgattcga tctgaattaa tttccaaagg tttgaagtgg gtcaatttac tttccaagga
     2881 tggattattt aaatttccag cggatgcaag ttttagttta gatgtaaaaa atatgggcta
     2941 tttagttggt tggtccaatc gttatatggc tttgaattgg ggatttaatg aaagagtcat
     3001 tgcaaaagag aaagagtatt acgaaaagaa ttggaaggaa aagagtaatt taaaaataga
     3061 tggaattagt ctggataaaa cggagagtcg agagactaac gattcagttt ctcaacagac
     3121 aatagcaaaa ttacaaattg aaaactatga tctaagacaa aaaatattta ctctaggagg
     3181 ggaatccatt caactctatt ttgatactgg aaatggtttt tcggaatcgg actctgaaaa
     3241 agttttgatt gagcaggaca aaaagaaata tgaaatcccg ctcgatgaga gtaagaatta
     3301 tttacaattt agactggata tttcgaataa tcctattaaa gtgatgctaa atagtattta
     3361 tatcattgat agaaacaact ctgaatacaa gttaaagata aaagagagca atggagtttt
     3421 agtaaatgag agagaatata tcttctcaaa cttcgatcct caattttatt tggagccttt
     3481 ttctaaacca atccaaatta aaaaatttgt gttagaatgc agtcaaattg agagtaatgt
     3541 tttggtgtat caaacagcct tacagatttc caatcaagcc aaagagagta tagtagaaga
     3601 aaaaaataaa cggattgagt ccattctaaa ctccaagcct tatattctag gaagtttaat
     3661 tgttaaacca ttcgatataa attcttggaa aaaattattt catagtatta aaaaaatact
     3721 aagtcagttt aggaattaaa caatggaaga gaaaatggat attaaagtcc gacaaatatt
     3781 ttttaaaaaa gaacagatca ataaagtatc agacaattgt tttccctatt ttaatagttc
     3841 tgattttgaa tcaaatttgt ttttagaata cagtgtaatt ttaaaatctt atttagatgg
     3901 tttttataaa gaaggagatt tggctggagt tttatcctgg cgtttttttg agaaagcaag
     3961 aaaaacagaa tcggagttct ttgactttgt ccgtaaaaat ccaggctatg atgtgtattt
     4021 tattaatcct tttatggagc aggtaaccat gtatgaaagt gtttggcacc aaggaggaac
     4081 caatcatcca ggtcttatag aagtagcaga tcgaattttt caagatctaa actatgggtt
     4141 taaaacatcg gatgtaaaaa atacgacagc cgatacgctt tactgtaatt actggattgg
     4201 aacacctaaa ttctgggaaa aatatattgc ttttacaaaa ccaatccatg attatatttt
     4261 agaaaataaa aatgatcccg aaacgataaa gaaattgaaa agcaatgcac cataccatta
     4321 tccagtatct tacattccat ttttaatgga gagattattt tctacattat tatggaaaga
     4381 ttcaacgatc aagtatttag gatatcaata ctctccatta gaaattgcct atttgatcaa
     4441 tgaattaaga ggatatgaat acgcgtataa aaaaattgat gtgtggtaca atctatttca
     4501 tcaaaacaaa atcatcctat ttttgaaaca tataagaaac tttttattaa gagtaaaagc
     4561 atttattaaa agaaatacca atgcagacta aagaatcgga aaaagtaaat ataggaatca
     4621 ttggctcaac cggtttttta ggtaggagtc taacaaatta tctttctcaa aaatattctg
     4681 ttcatgtcta ttctagaaga gagccgagtg atctgtttga aaatatttcg agttggacga
     4741 taggacaact gcatgatgag aaagtgctac aaggtttttt agagagaata gaaatactaa
     4801 tccatttagg gcagtctggc aagccaatgg aatcaaataa taattttgta tatgagacag
     4861 aggcaaattt aattccaagt ctacaattaa ttgaagttct aaagaaaact aaaaattcaa
     4921 ttcatattct atttgcaagc agtggcggaa ccgtctatca agaaaatcca gatcgcttct
     4981 atttagaaac agatccaaca gaaccattga catcctatgg aattaataaa ctctctcttg
     5041 aatattattt taatctactc acaaagatat cttctcattc ttgttttacg ttacgaattt
     5101 cgaatcctta cggtgaatta ttgtcatctg gaagaaatca ggggtttatc ggtgttagtc
     5161 tcagccgaat tctagaaggg aaggaagttt taatttttga tgatgaaaac atggtgcgag
     5221 attatattca tctagaagat atgtgctctg catttgaaca agcaattcaa tttagaaaag
     5281 cgggatattc tacttttaat ataggaacag gagtagggac tagtttaaga gagattttag
     5341 ttattatgga aaagtatttt aaaaaaacaa ttccaagaac aattgtcaaa aaagattcca
     5401 tgatcaaact tcctagatgg agtgttttga atattcaaaa ggcgagagaa gaattaaact
     5461 ggaaacctca aattgaattg gattctggct tacaaaaact aatagagtcg attcaatgaa
     5521 agcattgccc aaagtatcga ttattattcc agttaaaaat cctggaaaga tactagaaag
     5581 agttttatcc tctatttacg atcagtctta tccgagttca tttgaaatta taataataga
     5641 ttctggttct gtggatggaa ctttagaatt tttaaatgag tcaaaaaaga ataatccaat
     5701 tttaaaagtc tattcgattc cttcatccga gtttggtcat ggtaaaactc gaaatttggc
     5761 aattcaaaaa tcttcaggag aattagttgc tgttcttacg catgacgcaa ctcctgtaaa
     5821 tcaatactgg ctttcgaatc tagtccaacc atttcttgaa aatgattctg tggcaggagt
     5881 atttggaaga cattatgcgt atgaggatgc agatattttt acaaaacggg aattgaatca
     5941 acattttgat ggattcaaca gaactcctcc gattacctat tataagattg aagattttga
     6001 cagttattac aaaaacaaaa atttacagat gttctattgt tttttctcag ataacaacgc
     6061 gatgcttcga aaatctgttt gggaaaaaat tccctatgaa gatgtaaatt ttgcagagga
     6121 tcaaaaatgg gcaaaggcta ttctcgaaaa tggttataca aaagcctatg cagatcaagc
     6181 gatcgtctat cattcgcata attataccat tacagaagtg tttagaagaa gctttgaaga
     6241 ggcagtgagt tttcaattgc attttcaaca tagagtgatc gaaagtattt taacacttgt
     6301 aaaaattatt ttaatccgaa ctcgaatcga atactcagtg actatacgct ctagggatct
     6361 aagttttttt aaaaaaatat atctattatt caatagaccc atctacaatc tatttcgctt
     6421 tggtgggtat tatttgggat cgaatcgaaa gataagtaat cttttataca atcgagtgtc
     6481 gctcgatcat aaattgttta cggaaggaaa agggaggagt aaagacaaaa acaatgacta
     6541 gaatcattca aattatacaa tacatataca gactgattgc aaaaaaaata aaagttctac
     6601 tgcgggattt cattttatac attgtgtctc ccaaagatga tttggtatca aaggtttatt
     6661 atatattcat tgcttccaaa tatggaactc cttatacact aaataaaaat gaattagacg
     6721 ttaactggtt tattccagcc ttcaatcctg gatctggatc ggggggacat ttaaacattt
     6781 ttagaatggt tcatttttta gaaaaatata gaattaaaaa caatcttgtg attgttgaaa
     6841 gcaatcattt aacaaagaaa caaagtaaaa aaattatcaa tgaagattat atacccgttc
     6901 aagctgagct ctatgaagga aggtctgaag caaagcctgc tttgatttca atggcgacag
     6961 aatggaggac agcttatcct gttcgagatt ttattggatc tccgattaaa acatattttg
     7021 ttcaagatta tgagccactc tttt
//
LOCUS       DAOQOS010000199         6819 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_6361_length_6819_cov_5.937020, whole genome shotgun sequence.
ACCESSION   DAOQOS010000199 DAOQOS010000000
VERSION     DAOQOS010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 6819)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6819)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6819
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6361_length_6819_cov_5.937020"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            282..533
                     /locus_tag="PLF66_24735"
     CDS             282..533
                     /locus_tag="PLF66_24735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003005223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HMZ67317.1"
                     /translation="MPTYDYKCNTCNKVFEHFQSIKEEPLTKCLCDDKGDVTRMISSG
                     SGIIFKGSGFYVTDYKKSGSDTSSTSSSSSTSSSSSDKA"
     gene            539..1390
                     /gene="lpxI"
                     /locus_tag="PLF66_24740"
     CDS             539..1390
                     /gene="lpxI"
                     /locus_tag="PLF66_24740"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004765980.1"
                     /note="LpxI, functionally equivalent to LpxH, replaces it
                     in LPS biosynthesis in a minority of bacteria; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase LpxI"
                     /protein_id="HMZ67318.1"
                     /translation="MGRLGIIAGAGDLPHIAMREAIRQGEDPIFFSILESDFHPGDFK
                     ERCMPVYITQIGKIFKLCKKNNVDRILLLGKVKKEIILKTYRFDLKTITILAKMLNQN
                     DYTFFEVAAKEFDKEKIQIISQKTYLKPLLLPEGRYTKSKLSKSKLEDIEYGMNMAHN
                     LATLDIGQTVVVTQKMILAVEAIEGTDETIKRGGELSRKKGAVVCKSTKANQDERFDL
                     PTIGIDTLQNMHSSGCDTIAIRANETIVVNPKEFIQEANKLKINFISYSGPESHKYNA
                     EKKIQTY"
     gene            1362..2657
                     /gene="lpxB"
                     /locus_tag="PLF66_24745"
     CDS             1362..2657
                     /gene="lpxB"
                     /locus_tag="PLF66_24745"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016544527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HMZ67319.1"
                     /translation="MQKKKSKPTSKKKIIPKKTPTKTKISTKKKILEQDISISEPRKR
                     IEEKILIVCGEPSGDLLGADLMVELRNTKGNFSFTGIGGLEMGKLGFTSLYDIEDLSV
                     MGFTGIFKSYFKLKKIAKNLVEKAIIENVHYAVLIDYPGFNLYLAERLREKGIKVIFY
                     VSPQIWAWRFKRIFKIKKFVNLMLVLFPFEKKIYDEYGVKCEFVGHPLRNRMEEKMKS
                     EKPVQIDKGITTICLMPGSRGVEIDRLLDPILDGAVLIWERYKNLNKKVQFILPNINQ
                     SKELLIQEKLKSLKDEKGIDIQYFFDRSAKSIEASDLVILSSGTATLEVTFFEKPMII
                     LYKVGFFTYEIGRRIVSAKFFGLPNVLSEKLICKEYLQNEVTGETIYQEAINILEKDS
                     YRSEMIQNIKAVKQSLSDGHAGKNAANAILKLIQESVLL"
     gene            complement(2620..3006)
                     /locus_tag="PLF66_24750"
     CDS             complement(2620..3006)
                     /locus_tag="PLF66_24750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67320.1"
                     /translation="MSKIKKITAFVICIIAFSFCSKSEEKKQMFELYDTRELIGTWKI
                     VPSYSDKILFEKNQKTFLIHANQKTEIYALEDARGLRLQFKPEDDNPFGYFLFTEKTK
                     SIWGGVLDNKIVRIVREERIPESILE"
     gene            complement(2985..5198)
                     /locus_tag="PLF66_24755"
     CDS             complement(2985..5198)
                     /locus_tag="PLF66_24755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67321.1"
                     /translation="MINRFRIKIARRIEKLKEQYDYFRFRYLRSKWNVTILISLLILF
                     VSLFVANNFYAYYMTKNYINIFQVKSTIVSFIRKNLGKAIEIGIADFSSLQGIIFEDI
                     KISQEEDFSNNKLLFTSKRVDVRLTSLFSKAVVPNKIFVYSAKIILDIDDPINTNVLK
                     YIHELNLPDIYFDNLEIQIKSGNQDLLRSVENINVSISRREQFLDITFNDSQFFGLVS
                     GSVKGSGKINLESGENTLRIDFSKHDLDAMEGITNTLINLVPESGYAEGFISLNKTKE
                     DFSLDGNLNFRNFSGDFFTISNIRAKDLTINAKFSYLKEFIDQKTSQSYFKRKISTTE
                     FFYEEQIDTPPNNLKKTTVAIQVNNLDKLFDRLIFEDDFQVKGNMKINMRLEETGKVN
                     DWIWIDGNGSIGNLEIYSKNPEFSLTNTTLKFNWNQNELHSEILGKLFDQGFTSELNG
                     VISFLRQSKETPTTGFASNLKWNTKVDRLILKSFQSIYDAIDKKISGDIKERQEKMLP
                     ESFFIQSTIYKVFLEKANVKKHIEFEDVKYRAYSHSLGKYIADLKIEAGAGNLNLSGG
                     SFSKSENEMNLNFYYDRKMPSFDFRVKLGSLLWFDETLQICDSSVYSDIINFNMAFIS
                     SGNNFSDLLANRSINGELSLIGNSFIRTNPADNINLSEIFQGQNNLNISMGFNAYSQE
                     GFYRNIEIKSKENDLRGNANMIRSDVNYFLYGQIKNKPYNLSFTKTGASCQKLKK"
     gene            complement(5201..>6819)
                     /locus_tag="PLF66_24760"
     CDS             complement(5201..>6819)
                     /locus_tag="PLF66_24760"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HMZ67322.1"
                     /translation="KLILSHISRIKNMGEEYKKSLEYSEKEVDHIALDVFPEYVEGLK
                     NINSVDFDDLILLPMRILKDFPEVREYYHKHFKYYMIDEFQDTNQVQYEFIKLLLTEK
                     NNLCVVGDDDQSIYGFRGSNLNLILNFEKDFADTKVVRLLQNYRSSMNILNAANSLIK
                     HNVNRREKKLWSEIHHLERPTYVERLNEKEEAIFVVDEIQNELLKSHTIGSEIAILFR
                     TNYQSRPFEEELRLRGLPYKLIGAYNFFDRKEVKDLVAYIRVIANPKDELSLMRILNY
                     PKRGIGQTSISKIQQKSIDAGIPIMEVLHKICEEPEFIPEIKRASAALIYEFLELLHK
                     YRSEFYTANKMTEILRKLIKDVGFEKEISSEETEEKVIKARMLNLSELTNMMSYFEEE
                     WDSENKPTLFDFIARLSLLTNDDDSNEKETRDNRIQLMTMHLSKGLEFDVVFLVGLEE
                     GIIPNSRVLEEDQSVDEERRLFYVGMTRARRKLFLTGASERKKYGEPMPAEPSRFLNE
                     IARDYIVFQTMREETSPEDFLAELEKLKSA"
BASE COUNT         2335 a         1120 c          962 g         2402 t
ORIGIN      
        1 ccctttgttt ccttccttta attttttatt tcatcaccct gataggttac gaattcccta
       61 atatactcaa attcaaaatt tctctttaga aatctctaat ttttatatga acgactacct
      121 aaaattgtat gcaaaaaaat agagattatt ttgaaaacca aatggaaaac atgtaaagag
      181 acacaagatt caaaaatttt taaacttgag gatttagagc attttgaaaa cttcgtttat
      241 gaaagtctaa tatataaaaa gtttttaagt gagagatata tatgccaacc tatgattata
      301 aatgcaacac ttgcaataaa gttttcgaac actttcaatc tattaaagaa gaacctctaa
      361 caaaatgtct ctgcgatgat aaaggagatg ttactagaat gatctcaagt ggaagtggaa
      421 tcatctttaa aggttctggc ttttatgtaa ctgactacaa aaaatcagga tctgatacaa
      481 gttccacatc atcctctagt tcaacctctt ctagttcttc agataaagcg taattctttt
      541 gggaagacta ggcatcattg caggagcagg tgaccttccg catatcgcta tgcgggaggc
      601 gattcgtcaa ggtgaagatc ctattttctt ttccattttg gaatctgact ttcatccggg
      661 ggattttaaa gaaaggtgta tgcctgttta tattacccaa attggtaaaa tttttaagct
      721 ttgcaaaaag aacaatgtag atagaatttt acttttggga aaagtcaaaa aagaaatcat
      781 actcaaaaca tatcgctttg atttaaaaac aattactata ctagccaaaa tgctaaatca
      841 aaatgattat actttttttg aagtagccgc caaagagttt gataaagaaa aaattcaaat
      901 catttctcaa aaaacttatc taaagccatt gcttctacct gaagggcgtt atacgaaatc
      961 gaagctttct aaatctaaat tagaagatat tgaatatggg atgaatatgg ctcataatct
     1021 tgctactcta gatataggac agacggtagt tgttacccaa aaaatgattt tagctgtgga
     1081 agcaatcgaa ggcacagatg aaacgatcaa gagaggcgga gaactttcaa gaaagaaagg
     1141 ggctgtagtt tgcaaaagca caaaagcaaa tcaagatgaa agatttgatt tacctacaat
     1201 cggaattgat acattacaaa atatgcattc atccggatgt gatacaattg caatccgtgc
     1261 caatgaaaca attgttgtaa accctaaaga atttattcaa gaagcaaata aactaaaaat
     1321 taactttatc agttattcag gtccagagag tcataaatac aatgcagaaa aaaaaatcca
     1381 aacctactag taaaaaaaag atcatcccta aaaaaactcc cactaagaca aaaatatcta
     1441 caaagaaaaa aattctagaa caggacatat ctatttctga accaaggaaa cgaattgagg
     1501 agaaaatttt aattgtttgc ggtgaaccat cgggagatct attaggtgca gacttaatgg
     1561 tagaactcag aaatacaaaa ggtaattttt cttttactgg tatcggtggt cttgaaatgg
     1621 gcaaattagg atttacctct ctttatgata ttgaagatct tagtgttatg ggtttcacag
     1681 ggatttttaa aagttatttt aaattaaaga aaatcgctaa aaacctagtc gaaaaagcaa
     1741 ttatagaaaa tgttcattat gcagttttga tagactatcc tggattcaat ttatatcttg
     1801 cggagcgtct tagggaaaag ggaattaagg ttattttcta tgtttctcca caaatttggg
     1861 cttggagatt taagagaatt tttaaaatca aaaaatttgt aaacttaatg ctcgtattgt
     1921 ttccttttga aaagaagata tacgatgagt atggtgttaa atgtgaattt gtgggtcatc
     1981 ctcttcgcaa ccgtatggaa gagaaaatga aatcagaaaa acctgttcag attgacaagg
     2041 ggataacaac gatttgccta atgcctggtt caagaggtgt tgaaattgat agactacttg
     2101 atcccattct agatggagct gttttaattt gggaaagata taaaaaccta aataaaaagg
     2161 ttcagtttat actaccaaat ataaatcagt ccaaagaact actcatacaa gaaaaactaa
     2221 aaagtctaaa agatgaaaaa ggaatcgata ttcaatattt ctttgataga tcagcgaaga
     2281 gtatcgaagc atccgatttg gttattttat catctggaac tgcaaccttg gaagtaactt
     2341 tttttgaaaa gccgatgatt attttatata aagttgggtt ctttacctat gaaatcggta
     2401 gaagaattgt aagtgctaaa ttttttgggc tacctaacgt tctttccgaa aagctgatat
     2461 gtaaagaata tttacaaaat gaggtaactg gagaaaccat ctaccaagaa gcgatcaata
     2521 ttttagaaaa agattcttat cgatcggaaa tgattcagaa tattaaagca gtgaaacaaa
     2581 gtctcagcga tggtcatgca ggtaaaaatg cagcaaatgc tattctaaaa ttgattcagg
     2641 aatccgttct tctctaacaa ttcttacaat tttattatcg aggactccac cccaaatgct
     2701 tttggttttt tctgtaaaaa gaaaatagcc gaatggattg tcatcttccg gtttaaattg
     2761 caatcttaaa cctcttgcat cctctaatgc atagatttca gttttttgat ttgcatgtat
     2821 taaaaatgtt ttctgatttt tctcaaataa tatcttatca ctatacgaag gaacaatctt
     2881 ccatgttcca attaattctc tagtatcata taactcaaac atttgttttt tttcttcgct
     2941 cttactgcaa aaagaaaaag ctattataca aattacgaat gctgttattt ttttaatttt
     3001 tgacatgatg ctcctgtttt agtaaatgat aagttataag gtttattttt tatctgaccg
     3061 tatagaaaat aatttacatc agatcgaatc atattggcat ttccacgtaa atcattttcc
     3121 ttacttttaa tttcaatatt tcgataaaaa ccctcttggg aatatgcgtt aaatcccata
     3181 ctaatattca aattattttg accttggaat atttctgata aattgatatt atcagcagga
     3241 tttgttcgaa taaaactatt tccaatcaga cttaattctc catttatact tctattcgcg
     3301 agtaaatctg aaaaattatt gccactagaa ataaatgcca tattaaaatt tataatatct
     3361 gaataaacac tactatcgca gatttgtaat gtctcatcaa accacaataa agatccaagc
     3421 ttgactctaa aatcaaaaga aggcattttt cgatcgtaat aaaaattcag attcatttca
     3481 ttttcactct ttgaaaatga cccacctgat agatttaaat tgccagcacc agcttcaatt
     3541 tttagatctg ctatatactt tccaagacta tgcgagtatg ctctatactt cacatcttca
     3601 aattctatat gcttttttac atttgccttt tctaaaaata ctttgtaaat agtagattgg
     3661 ataaagaaag attcaggtag cattttttcc tgtctttctt ttatatcacc actaattttt
     3721 ttatcaattg catcatagat agattgaaaa ctttttagga ttaacctatc tacttttgta
     3781 ttccatttta aattggaagc aaatcctgtt gttggagtct ctttcgattg acgcagaaat
     3841 gaaatcactc cgtttaactc agatgtaaaa ccttgatcaa atagtttacc taaaatttct
     3901 gagtgtaatt cattttgatt ccaattaaat ttgagcgttg tatttgtaag actaaactct
     3961 ggatttttac tatatatctc taaattccca atcgagccat tcccatcaat ccaaatccaa
     4021 tcatttacct tacctgtttc ttccagtcgc atattgatct tcatgtttcc tttcacttga
     4081 aaatcatctt caaagattaa acgatcaaat aatttatcta aattattcac ctgaatcgct
     4141 acagtagttt tttttagatt gttaggtgga gtatcaatct gttcttcata gaaaaactca
     4201 gttgttgaaa tttttctttt aaaataagac tgagatgttt tttgatctat aaattctttt
     4261 aaataagaaa attttgcgtt aatcgttaga tctttagctc ttatatttga tattgtaaaa
     4321 aaatctccag agaaatttct aaaattcaaa ttcccatcta aactaaaatc ctcttttgtt
     4381 ttatttagag aaataaagcc ttccgcataa ccagactctg gaactaaatt tattagagta
     4441 tttgtaattc cttccatcgc atctaaatca tgtttagaaa aatcaattct taatgtattc
     4501 tctcctgatt ctaaatttat tttccctgat cctttaacac taccagatac aagtccaaaa
     4561 aattgggaat cattgaaggt tatatcaagg aactgttctc tcctagagat agaaacatta
     4621 atattctcaa cacttcgaag caaatcttga tttccagatt taatctgaat ttccagatta
     4681 tcaaaataaa tatcaggtaa gttcaattca tgaatgtatt tcaaaacatt tgtgttgatt
     4741 gggtcatcta tatctagaat aatttttgca ctataaacaa agattttatt tggaactaca
     4801 gcttttgaaa ataatgaagt tagcctcaca tccactcttt tacttgtgaa taataattta
     4861 ttattagaaa aatcttcttc ttgtgaaatt tttatatctt caaaaataat tccttgcagt
     4921 gatgaaaaat ctgctattcc tatttcaatt gcttttccta aatttttacg aataaatgaa
     4981 acgatagtcg actttacttg aaaaatattt atataatttt tagtcatata ataggcataa
     5041 aaattatttg cgacaaatag actcacaaat aagattaata gcgaaattag aattgttaca
     5101 ttccatttag atcttaaata cctaaaacga aagtaatcat actgctcttt aagcttttct
     5161 attcttcttg ctatctttat tctaaagcgg ttaatcataa ttatgcggat tttaattttt
     5221 cgagttctgc taaaaaatct tcaggacttg tttcttctct cattgtttga aaaactatat
     5281 aatctctcgc tatttcattt aaaaagcgag aaggctctgc tggcattggc tctccatatt
     5341 tttttctttc ggatgcgccc gttaaaaata attttcttct agctcgggtc atgcctacat
     5401 aaaatagcct acgctcttca tctacacttt gatcttcttc taaaactcta gagtttggaa
     5461 taatcccttc ttctagacca actaaaaaaa ccacatcaaa ttctaatccc ttggaaagat
     5521 gcattgtcat tagctgaatt ctattatctc tagtttcttt ttcattagaa tcatcatcat
     5581 tagttaaaag agaaagtcta gcaataaaat caaataatgt cggtttgttt tctgaatccc
     5641 actcttcttc aaaataagac atcatattgg ttaattcgga tagatttaac attcttgcct
     5701 taatcacttt ctcttcggtt tcttccgatg aaatctcttt ttcaaatcct acatctttaa
     5761 tcagtttcct aagtatttcc gtcattttat tagcagtata aaactcggat ctatacttgt
     5821 gtaataattc taaaaattca taaatcaaag cagctgatgc tctttttatt tcaggtataa
     5881 attcaggttc ctcacaaatt ttatgcagaa cctccataat aggaattcct gcatcaattg
     5941 atttttgttg aatcttagaa attgaagttt gccctattcc acgttttggg taatttagaa
     6001 tacgcataag agacaactca tcttttggat ttgcaattac acgtatatat gctactaagt
     6061 ctttcacttc ctttcgatca aagaaattat acgcgccgat caatttatat ggcagtcctc
     6121 ggagtcgtaa ttcttcttca aagggtctcg attgataatt agttctaaat aaaatggcaa
     6181 tctccgatcc tatcgtatga ctctttagta attcattttg aatttcatct acaacaaaaa
     6241 tggcttcctc tttttcattt agtctttcta cataggtagg tctttctaaa tgatgaattt
     6301 cagaccatag ttttttttct cttctattca cgttatgttt aatgagggaa tttgcagcgt
     6361 ttaaaatatt catagaagaa cgatagtttt gtagtaaacg cacgactttg gtatcagcaa
     6421 aatccttttc gaaatttaaa attaaattta gattagatcc tctaaatcca tatatacttt
     6481 gatcatcatc tccaacgaca catagattat ttttctctgt caataagagc ttaataaatt
     6541 catattgaac ttggtttgta tcttgaaatt catctatcat ataatattta aaatgtttat
     6601 gatagtattc cctaacttcc ggaaaatcct ttaaaattct cattggaagt aatattaaat
     6661 catcaaaatc aacagaattt atatttttaa gtccttctac atactcagga aaaacgtcta
     6721 gcgcaatatg atccacttct ttttctgaat attctaaact ctttttatat tcctcaccca
     6781 tatttttaat acgacttata tgagataaga taagtttag
//
LOCUS       DAOQOS010000200         6805 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_6380_length_6805_cov_7.424000, whole genome shotgun sequence.
ACCESSION   DAOQOS010000200 DAOQOS010000000
VERSION     DAOQOS010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 6805)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6805)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6805
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6380_length_6805_cov_7.424000"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..266)
                     /locus_tag="PLF66_24765"
     CDS             complement(<1..266)
                     /locus_tag="PLF66_24765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67323.1"
                     /translation="MKKKDQDVELKFNDAYDDNAENVSNKKEQVILSPDINNKKTAFS
                     GIVVFIIIFSFIALIVSTISYFIGGSNNNSFKSFQRKINTDNKI"
     gene            complement(275..682)
                     /locus_tag="PLF66_24770"
     CDS             complement(275..682)
                     /locus_tag="PLF66_24770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67324.1"
                     /translation="MSSLNQLFNLLKASTILGTGSLAGSFAIIVDGVNVKFKFDFGDF
                     HRERTSNTNLSSISEDQCDKVRGFFESIKRNITEKELRNYYAGIKDIKIQGKTLEHVS
                     YVICHTGLTILKATNGKQQIIDQYEAHYKKIKK"
     gene            complement(679..1611)
                     /locus_tag="PLF66_24775"
     CDS             complement(679..1611)
                     /locus_tag="PLF66_24775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67325.1"
                     /translation="MNIPITSIKDAVKSLLQLLHFSTIIPVSLFLVILNYIILPGWNP
                     NKELNSKENLNYEIVGTFLFIFLCYLLYAYNHLLIRFIEGYTLRNFLGSFELGFLNKY
                     YELKESLSKASEKDKIAAIEKLHFEFPVQEELILPTRLGNIIAAFENYSFDKYGIDAV
                     LLWPRMIPILEKENYLNIIAQEKGIFDFLLNLFYVILASGLLVMSSFSYNHNGSGLFF
                     SFIITLISLYVIYNGLISSAIAWGNTIKVSFDLYRNQLWKQLRLTMNCSIDDELVIWR
                     EFSRFIREKSKSNFYTELNIVTLSSFNEYLKEKK"
     gene            complement(1618..5061)
                     /locus_tag="PLF66_24780"
     CDS             complement(1618..5061)
                     /locus_tag="PLF66_24780"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3883 domain-containing protein"
                     /protein_id="HMZ67326.1"
                     /translation="MKKGDTLISPIFPEQVELIQVEVVGDSYTLFCKGLSGQFYERHL
                     TEAQFKSITVLVKGDYSGDSKKFRIAVEAYRLGLAYEYDPYFSLSISRVDPLPHQLEA
                     VYDYFLKAPRIRFLLADDPGAGKTIMAGLLIKELKIRGLIKRILIITPANLTFQWQRE
                     MKDKFKENFDLIRGDVLRANYGVNPWQEKNQVISSVTWASQVDDANQSLLRANWDLVI
                     VDEAHKMSANTDDKKTFAYKLGEELSERTDHLLLMTATPHKGDPEHFRLFLQLLDKDI
                     YANMQSLQLAMEQREAPFYLRRVKEALVSFPDPETGKSKSLFTKRNVRTLDFQIQDEE
                     WDFYNTLTRYVEENSARAAQENTAAARAIGFTMAMLQRRFASSMYAIRRTFERMKERR
                     EEILKDPEGFKKKQIERKLPDNFEDLPEEEQEELLESLEETVISYNPFDLKREINELR
                     ILIEKAIDLEKKDIEEKVKKLKEVLHKEGLFKDKNIKLLIFTEHRDTLDYLAGDGQNG
                     RPTGKLVQWGFDVTTIAGGMKIGDRNSPGTRIFSEWDFRENKQILVATEAAGEGINLQ
                     FCWLMINYDIPWNPVRLEQRMGRIHRYGQEKDCLIFNFVATNTREGRVLAKLFQKIEA
                     IEEDLDPQRTGKIFNVLGDIFPSNMIEKLIRDMYARNKTEDVVTSRIIEQVDSERLRQ
                     ITNSTLEGLAKRELNLSAIIGKSEEAKEKRLVPEVIEDFFREAAPLHGIQLKEVSKDT
                     HVFTVGKVPRNLWQRGEKNESRFGTLGKEYGKIIFDKQKLAKDNLLEWVTPGHPLFEA
                     VREESLEKCRESLDKGAVFFDLYRNEPARLNLFLVAMSDGLGRVLHQKLFVTETKLNS
                     SVHLKQPTVFLEIQPGECKEDDKKFILEKDRSIPKIEELKKYLLSNGLNEFQALIEKE
                     RRKEIHTVEKHIDISLSELINRQNIRYAEMTEKKEAGDTSPQLLGHLAQTEHRILELE
                     NRKEIRFKELAREKEISISSIQHLGSAWVIPHPDKNKPEIQKMVSNDDIEKIAVDAVI
                     AFEQERGFNVQSVEADNRGFDLISRLPHPEDKESTKEVRFIEVKGRSLVGEIALTANE
                     YRTSQRLQNDYYLYVVYNCSTKPEIHVIQNPAKMGWQPVVQIEHYHVKAEEILRKSER
                     G"
     gene            complement(5322..5492)
                     /locus_tag="PLF66_24785"
     CDS             complement(5322..5492)
                     /locus_tag="PLF66_24785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67327.1"
                     /translation="MADKEKTIFSIQVPNEMNAELEEETKRKSRSRNFIVNLALKLFL
                     EKPEKERDKLYP"
     gene            5644..6324
                     /locus_tag="PLF66_24790"
     CDS             5644..6324
                     /locus_tag="PLF66_24790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67328.1"
                     /translation="MNQFTDYTPKRKPKTETVKEEADNSHFPQSGNRGNAIGKVFILV
                     GVGIGLILFMTLLGTSFSGNDLLTLRNLENKIDEGISLTKEEQILYCRLLQSIRNISI
                     ENCESVDLKTIFSSGRILSKNSKKNCDKVDYLFKDKNKALIWARKNLGHDTEKIYDIQ
                     RGILVEWINERGDKVYWNHGDWGSDPGKSRFPHLNYSIGNCNGHLFLKDKIVNRGQWK
                     EFAEQFQL"
     gene            6321..6749
                     /locus_tag="PLF66_24795"
     CDS             6321..6749
                     /locus_tag="PLF66_24795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67329.1"
                     /translation="MNTKELTLDQIISTSGDSTLLSYKYENQVLEFTLSHEELDRTIS
                     FRIQTGDLKALIYENYPNDSTSKTCYIELLELSQALNQTNHHYIPSSDFGKMMQECKA
                     GLNLAYGKKVEANPYLLSLKGRETLLSCLVSDLSHISFTL"
BASE COUNT         2144 a         1305 c          977 g         2379 t
ORIGIN      
        1 ataattttat tgtctgtatt tattttacgt tgaaaagatt taaaagaatt attattactt
       61 ccaccaataa aataacttat tgtagaaaca attaatgcta tgaatgaaaa aattattatg
      121 aatacaacta ttccagaaaa agctgttttc ttattattta tatcaggact aagtataact
      181 tgctcttttt tattactaac attttcagca ttatcatcgt aagcatcatt aaattttaat
      241 tcgacgtctt gatctttctt tttcataatt taatctattt ttttatcttt ttataatggg
      301 cttcatattg gtcaataatc tgttgcttcc cattagtggc tttaagaata gttaagccag
      361 tatgacaaat aacgtatgag acatgctcta aagtcttacc ttgtattttt atgtccttaa
      421 ttcctgcata ataatttctt aattcttttt ctgtaatatt cctttttata ctttcaaaaa
      481 accctcttac tttatcacat tgatcctctg aaattgaact caaattagta ttggatgttc
      541 tctctctatg aaagtcacca aaatcaaatt taaattttac atttactcca tcaactataa
      601 tagcaaatga gcctgctaag ctacctgttc ctaaaattgt tgaagctttt aaaagattaa
      661 ataattgatt aagactgctc attttttttc ctttagatac tcattaaaac tggaaagcgt
      721 cacaatattt aattccgtat aaaaatttga tttcgacttt tctcttatga atcttgaaaa
      781 ctctctccat attaccaatt catcatctat agaacaattc attgttagcc tcaattgttt
      841 ccatagttga ttcctataca gatcaaaaga aactttgatt gtattacccc atgctattgc
      901 agaactaata agaccattat aaattacata tagcgatata agagtaatta taaaggaaaa
      961 aaataaccct gatccattat ggttataact aaaagaagac attactaaaa gaccggacgc
     1021 tagtattaca taaaataaat ttaataaaaa gtcaaaaata cccttttctt gggctattat
     1081 attcagataa ttctcttttt ctaggatagg aatcattctt ggccaaagta atacggcatc
     1141 tattccatat ttatcaaaag aataattttc gaaagcggct ataatattcc ccaaacgagt
     1201 tggtagtatt aattcttcct gaactggaaa ttcaaagtgc aacttttcta tagcggctat
     1261 tttatctttt tcactggctt tcgataagct ttcttttaac tcataatatt tattaagaaa
     1321 tcccaattca aaagatccta agaaatttcg taatgtataa ccttctataa atcgaattaa
     1381 aagatgatta taagcataaa gtaaatagca taaaaaaata aaaaggaaag ttcctacaat
     1441 ttcataattc agattttctt tggaatttaa ttctttattt ggattccatc ctggcaatat
     1501 aatataattt agtatcacca aaaaaagaga gacgggtatt attgtagaaa aatgaagcaa
     1561 ttgcaataaa gattttactg catctttaat cgaagtaatc ggtatattca taatctatta
     1621 tcctctctca ctcttacgca atatctcttc tgctttcaca tgatagtgct caatttgaac
     1681 aactggttgc caacccattt tcgctggatt ttgtatgacg tgtatctctg gctttgtgga
     1741 acaattgtaa accacataca gataataatc attttgcaat cgctgagagg ttcgatattc
     1801 atttgcggtt aatgcaattt caccaaccaa tgaacgacct tttacttcaa taaaacgaac
     1861 ttctttagtg ctttctttat cttcaggatg cggtaggcgc gagattaaat caaaaccacg
     1921 attatccgct tcgacacttt gaacattaaa gcctctttct tgctcaaatg caatcactgc
     1981 atcgactgca atcttttcaa tgtcatcatt gctcaccatt ttttgaattt caggtttatt
     2041 tttatcagga tgaggaatta cccaagccga accaagatgt tgaatagaag aaatagaaat
     2101 ttctttttct cttgccaatt ccttaaaacg aatttctttt ctattttcta attcgagaat
     2161 ccgatgttct gtttgcgcta aatgccccaa tagttgaggg gaagtatctc ctgcttcttt
     2221 cttttcggtc atctctgcat aacgtatatt ctgtcgattg atcaattcac ttaggctaat
     2281 atcaatatgc ttctcgactg tatgtatttc ttttctgcgc tctttttcta tcaaagcttg
     2341 aaattcattc aacccattgc taagtaaata tttttttaat tcttcgatct ttggaatcga
     2401 acgatctttc tctaaaataa attttttatc atcctccttg cattctcctg gttgaatttc
     2461 taaaaatact gttggttgtt tcaaatggac gctagaattt aattttgttt ctgttacaaa
     2521 taatttttga tgtaaaactc ttccaagacc atcggacatt gcgactagaa ataaatttaa
     2581 acgagctggc tcatttctat ataaatcaaa gaagacagct cctttatcaa gagactctct
     2641 acacttttct aaactttcct ctcgcaccgc ttcaaatagc ggatgaccag gagttaccca
     2701 ctccaaaaga ttatccttgg ctaatttttg tttgtcgaaa atgatctttc cgtattcttt
     2761 tcctaacgtc ccaaatctgc tttcgttctt ttctcctctt tgccaaaggt tacggggaac
     2821 tttgcccaca gtaaaaacat gagtatcttt agaaacttct ttcaattgaa ttccatgtaa
     2881 aggagcagct tctctaaaaa aatcttctat tacctctgga actaatcgct tttcttttgc
     2941 ttcctcggac tttccaatga tggcagataa attcaattct cgttttgcaa gaccttccag
     3001 tgtggaattt gtgatttgcc gcaaccgctc tgaatccact tgctcaatga ttcgacttgt
     3061 aacaacatct tcagttttat ttcgagcata catatctcga attaattttt caatcatgtt
     3121 agaaggaaaa atatcgccta acacattaaa tatttttcca gttctctgtg gatctaagtc
     3181 ttcttctatt gcttctattt tttgaaataa ttttgcaagc actctacctt ctctagtatt
     3241 ggtagcaaca aaattgaaaa tcaggcaatc tttttcctgt ccataacgat gaatacgccc
     3301 catcctttgt tctaatcgaa ccggattcca aggaatatcg taattaatca taagccaaca
     3361 gaattgtaag ttgattcctt ctccggcagc ttccgtcgca acaagaatct gtttattctc
     3421 tctgaaatcc cactcggaaa agattcttgt gccaggagaa tttctatcac caattttcat
     3481 tccgcctgcg atagtcgtaa catcgaatcc ccattgaact aatttcccag taggtcttcc
     3541 gttttgccca tcccctgcaa gataatccaa agtgtctcta tgttctgtga agataagaag
     3601 ctttatattt ttatctttaa ataatccttc tttatgcaaa acttctttta gtttttttac
     3661 tttttcttct atgtcttttt tttctagatc aattgctttt tcaattagta tccttaattc
     3721 attaatttct ctttttaaat cgaacggatt ataactaata accgtttcct ctaaactctc
     3781 caataattct tcttgctctt cttctggaag atcttcaaag ttatccggta gctttctttc
     3841 aatttgcttt tttttaaaac cttctggatc tttgagaatc tcttctctac gttctttcat
     3901 ccgctcaaat gttctacgaa tagcatacat actagaagca aatcgtcttt gaagcatcgc
     3961 catagtaaaa ccgatagctc tcgccgccgc agtattttct tgtgctgcac gagcggaatt
     4021 ttcttcaaca taacgagtaa gagtattata aaaatcccat tcttcatctt gaatctgaaa
     4081 atctaatgtt cgaacatttc tctttgtaaa aagggatttt gatttaccag tctcggggtc
     4141 aggaaaggag accaatgcct cctttaccct acgcaaataa aagggagctt ctctttgttc
     4201 cattgcaagt tgtagacttt gcatattagc atatatatct ttatctaata attgtagaaa
     4261 taatcgaaag tgttctggat cacctttatg cggagtggct gtcattaaga gtaaatgatc
     4321 tgttctctcg gaaagctctt ctcctaactt gtatgcaaat gtttttttat catccgtatt
     4381 cgcactcatt ttatgagctt catcaacaat tactaaatcc caattcgcac gcagcaaact
     4441 ttggtttgca tcatctacct gtgatgccca tgtaacagag gaaattactt gatttttttc
     4501 ttgccaaggg tttactccat agtttgctct tagaacatct ccacggatta aatcaaaatt
     4561 ttctttgaac ttatccttca tctctcgttg ccattggaat gtaagattag cgggggtaat
     4621 aatgagtatt ctctttatca agcctcttat ttttaattcc ttaataagaa gacctgccat
     4681 aattgtttta ccggcaccag gatcatccgc caacagaaaa cgtattcttg gagctttcaa
     4741 aaaataatca taaacagctt ctaactgatg aggtaaagga tcaaccctcg aaattgataa
     4801 agaaaaataa ggatcgtatt cgtaggctaa acctagtcta taagcctcta cagcaattct
     4861 aaatttctta gaatccccag aataatcgcc tttcacaaga actgtaatac ttttaaactg
     4921 agcctcagtt aaatgcctct catagaactg accagacagt cccttacaaa aaagggtata
     4981 gctatcgcca actacttcaa cctgaatcaa ttcaacctgc tcaggaaata ttggacttat
     5041 tagcgtatcg ccttttttca tattttaatc ttattttaac aaaagaaaac tttgggaata
     5101 atacaatata ttttcattat tgtaggtttg caaatttaaa attaaataca aaagtttaat
     5161 atcattagtt aatgaaatta aatcaaacaa accatcgacc tcgaccaaat gcaaataaag
     5221 agctaaacga tgttatgcat tattgatatt ttgacaagat aagggtcatc ttgtgaataa
     5281 aatttctaca tttgagatga accaaatgtc ggaaccactg cctacggata caacttatcc
     5341 ctttccttct ccggcttctc cagaaaaagt tttagagcga ggtttacaat gaaattcctt
     5401 gatcttgatt ttcgttttgt ttcttcttct agttctgcat tcatttcatt gggaacttga
     5461 atcgagaaga ttgtcttttc tttgtctgcc atactaaacc tctactttca atgaaaacta
     5521 tgaattttta actttcaatc actaaaatta ataattttaa acaaaaatta ttgtttacta
     5581 ttaacaataa ttaatatcta ttgttaattc atgttattgc tttaaagtaa aaggacacac
     5641 accatgaatc aattcacaga ctacactccg aaaagaaaac caaagacaga aacagtaaaa
     5701 gaagaagcgg ataattctca ttttccgcaa tcaggaaata gaggaaatgc gatagggaaa
     5761 gtatttattt tggtcggggt cggaattggg ttgattctat ttatgacgtt acttggcaca
     5821 tcattttcag gcaatgattt actgactcta cgcaatttgg aaaataagat tgatgaggga
     5881 atttctctta caaaagagga acagattctc tactgtcgac ttttgcaaag cattcgaaat
     5941 atttccattg agaattgtga atcggtagat ttgaaaacta ttttttcgag tggaagaatc
     6001 ctttctaaaa attctaaaaa gaattgtgac aaagtggatt acctttttaa ggataaaaat
     6061 aaggcactca tctgggctag aaaaaaccta ggacatgaca cagaaaaaat ttatgatatc
     6121 caaagaggga ttttagtaga gtggatcaat gagcgagggg ataaggtcta ttggaatcat
     6181 ggggactggg gaagcgatcc cgggaaatct cggtttcctc atctcaacta ttccattggg
     6241 aattgtaatg gacatctttt cctaaaagat aaaattgtaa acagagggca atggaaagaa
     6301 ttcgcggagc agtttcaact atgaatacaa aagaacttac actcgaccaa attatctcaa
     6361 ctagcggcga ctcaactctt ctctcctata agtatgaaaa ccaagtcttg gagtttacac
     6421 tctctcatga agagttagat cgaacgattt cttttcgaat tcaaacagga gacctcaaag
     6481 cattgatcta tgaaaactat ccaaatgact ctacctctaa aacttgctat attgaattac
     6541 tcgaactctc ccaagcgtta aaccaaacga accaccatta cattccttcc tctgactttg
     6601 gtaaaatgat gcaagaatgc aaagctggtc tcaatctagc ctatggcaag aaagtagagg
     6661 ctaatcccta tttactttcc ttaaaaggaa gagagactct tctttcctgt cttgtctctg
     6721 atttaagtca catttccttt actctctaaa ccctttcttc gtattacctt cgtattaacg
     6781 atacactatc tggctactat ctcgc
//
LOCUS       DAOQOS010000201         6755 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_6461_length_6755_cov_6.279254, whole genome shotgun sequence.
ACCESSION   DAOQOS010000201 DAOQOS010000000
VERSION     DAOQOS010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 6755)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6755)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6755
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6461_length_6755_cov_6.279254"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(197..559)
                     /locus_tag="PLF66_24800"
     CDS             complement(197..559)
                     /locus_tag="PLF66_24800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HMZ67330.1"
                     /translation="MARYEHLPIYKSAMDLAIEMEIQVRNMSRYNKYAIGAELRKRTL
                     AILSFVVRANSITDKSPVLEELRIVLEELRQLLFLSKETKALASFAIYKKIMEYAENI
                     SKQNEGWLRSQNIKGKKL"
     gene            complement(594..1700)
                     /locus_tag="PLF66_24805"
     CDS             complement(594..1700)
                     /locus_tag="PLF66_24805"
                     /inference="COORDINATES: protein motif:HMM:NF019237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HMZ67331.1"
                     /translation="MKIKLWIIVVSFVLLQGCTAALVSELTPNGGGSSGLTNLFVLLG
                     LNRRSGGSGVSYTPIDTYNFSSGVPSGWTGTWTSTSTSCPVTGTTPCLRSAVITNSQS
                     SVLSFTTTLKASKISFKRRVSSESNYDYCKFTIDGADVSGEGLGVSGTEDKSYEFIFS
                     EAASRTFAWTYRKDDSYFSGLDLCSLDDVVLYGVATETGTGTTTETVIPVVVLKTGQT
                     TSYATGDDGNLQKGAARSYTDNNDGTIKDNSTGLTWQKCSRGLSGLTCATGSATTSDW
                     TSAGSYCSSLTLASKTWRLPTVNELPNLVDASRSVAPIINITSFPATQSGYYWSSTTY
                     APVTSGAWVVDFNDGSVSSGGKTNSNYVRCVSGP"
     gene            complement(1703..2542)
                     /locus_tag="PLF66_24810"
     CDS             complement(1703..2542)
                     /locus_tag="PLF66_24810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67332.1"
                     /translation="MRNILKKLKCQFEQSKRQSKTTSRKSLYSISTSRLALKVTMSIL
                     LISLTNCVSVVQKKEYLEAPILVKPFTCSSSPGSEVEYIDCRFRTLTEKYTSRKQYLE
                     EEFEKAEPGNKKQIRLIISETDKKLKDLVSWKNANEPGARKRDEKWAAEEAKRQEEAD
                     RLARIEKIRQEKEEKEIQLAEMKRSGKKNPSECKRAKEGLQDCIQNARLGRQGAQVFG
                     ALTGVNTSAFQDVASNVETACRTFKATITIACAFAGESDIAEVMSEIKVLETEIREAE
                     KRR"
     gene            complement(2551..3249)
                     /locus_tag="PLF66_24815"
     CDS             complement(2551..3249)
                     /locus_tag="PLF66_24815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67333.1"
                     /translation="MKHRRSIYLFLILPIFCQCSIAEIRTPSVEEGSYLKSIRKFVLS
                     FPNKIEVEMKKATMRDSMESPPVLTEASKDGLEKSYSKSFTTAFTEYASSRIEAVPAD
                     RISFVEESETDKDRARILETLGVNARIEVKSKVTLDYPTRVPDLGVGNIFYFPLLLDL
                     WWIDLSGAWRIDHTLEFSVVDIKTGSVKFQSTPVTKGKSTRTFILSNLNSSEFNEAIE
                     KSTEEIILAALGKK"
     gene            3587..5569
                     /locus_tag="PLF66_24820"
     CDS             3587..5569
                     /locus_tag="PLF66_24820"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HMZ67334.1"
                     /translation="MKSVWKVLVIVSFSLSAGELPFAKNTVVLGEGDTWINIMSNLEE
                     LQDTSTDRSIHYPLSKKEDFKQVPSTMKIWSSPDTNIESGVWFRVKLRNENPNENMYV
                     LNDTYQAKLILFFLLDENGKILSEKKVGFGTDHKDSFLKFNSNSISFDLKYKEEVYLY
                     LFLYSEGFQFPNFDIFIWKEQIYFEHYRTIYLYLYYGIAISIFFYNLFLFFSTKDKAY
                     LHYCFYVLSVICFFFCYQQQVIFLPFAIVKNYGDYFIQIFAFFTIITMIKFALSFLNL
                     KSIYKITFFLLESAVKILYIYLILLLFLRIFLKSDNFIINNFMLIMQPTILIIDLLIT
                     YLAISAWWNGYIRAKYFTIAWILYLFGLFYAFLINFLNLANIQLFASWGIGFLILTHM
                     NMFLGSIEMLLLSFALGDRYNLIQQENERAKQALLEREIQLKEAEATKKKLELGMLKQ
                     TIQPHFLMNSLNTVLGLIRENPDKAGEFVSFLCMEIRTMLDVSSERLIPISSEFAICK
                     SHLEIMRIRLERKFTWKTQDISEEEKIPPLLFQTLLENAFTHSKGYTGNIAFLVKKLI
                     CDPEKPFPSGNIEWIQDQTLQALKRIESVYEFYSIYDESYKANTNLSQSNYRTGEDGT
                     GTLYIKSRLEESYGDNWSFSQGFEGKSWVVRIGVGK"
     gene            5667..6389
                     /locus_tag="PLF66_24825"
     CDS             5667..6389
                     /locus_tag="PLF66_24825"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HMZ67335.1"
                     /translation="MKTEKIKKNSAIKVLIIEDEPVTARYIQTLLREFYKSRNVSIFM
                     QKSLIGSQAFIEEQSIDLILLDLNINGEDGLEIMESPFIQSIQTIVISGNTERAIDVF
                     RHLVLDFVPKPVLPKRLFEALEKYEAKESWKKKGKHLSIKKDDMIELVSYETICYLKG
                     EGKTTKVFLKEGNPLTINKNINSVLMNLPDYFFRIHKSYVIPLNCINKIKLKPAFTVI
                     LKNGTELPVAKRVIKKLQEEVL"
BASE COUNT         2060 a         1304 c         1150 g         2241 t
ORIGIN      
        1 gaactccaat aattgttgga ctgagttgtc tttctactta ttgaccagaa taatccagtc
       61 caccaggaga atgttttgtt attcacaaag aaactctcct tgcatatttt atccctcata
      121 aagagaggag aaagacgcac cgtgatctta ggcactctca tcatcctttt agtatagaac
      181 aatgattctc gccttttcat aatttctttc ctttaatgtt ttggcttcta agccaaccct
      241 cgttctgttt agaaatattt tccgcatatt ccattatttt tttataaatg gcaaaagagg
      301 ccaaagcctt tgtttctttg gataaaaaca ataactgtct taattcttcc aacacaatgc
      361 gcaactcttc tagtactgga cttttatctg tgatagagtt tgccctgacc acaaaagaga
      421 gtatggctaa ggttctctta cggagttcgg ctccgattgc atacttattg taccgactca
      481 tatttctaac ctgtatctcc atctcgatag caagatccat tgcagattta tagattggca
      541 aatgttcgta tctagccatt atttttcaat ttccaaagga taaaattacc aagtcacggt
      601 cccgacacac aacgcacata attgctatta gtcttaccgc cgctgctcac actgccatca
      661 ttgaaatcga cgacccacgc accgctagta acaggcgcgt aggtagtaga actccaataa
      721 tagccggact gagttgcagg aaacgaagtg atattgatga taggagctac acttctgctt
      781 gcatctacta ggttcggaag ctcattcaca gtaggtaatc tccaagtttt actcgcaaga
      841 gttaggctag aacagtaact tcccgcactc gtccaatccg atgttgtagc agatccagtg
      901 gcacaggtta atcctgataa tcctctacta cacttctgcc atgttaatcc agtcgaattg
      961 tctttgatgg tcccgtcatt attatctgta taacttctgg cagctccctt ttgcaaattc
     1021 ccatcatcac ctgtcgcata agaagtagtt tgccctgttt ttaggacgac tacggggatt
     1081 actgtttctg tagttgttcc tgttcctgtc tcggtagcaa ctccatacaa cactacatca
     1141 tctagggagc ataaatccaa tccactgaag taactatcat ctttgcgata agtccatgca
     1201 aacgttctac ttgcggcttc agagaagata aattcatagg atttatcttc cgttcctgaa
     1261 acgcccaatc cttcccctga aacatcagct ccatcgatag tgaatttaca ataatcataa
     1321 ttagactcac tgcttaccct tcttttaaaa gaaatcttac ttgctttcaa agtagttgtg
     1381 aaggagagaa ctgaactttg actattcgtt ataacagccg atcgcaggca cggggtagtt
     1441 ccagtaacag gacagcttgt agaagtgctt gtccaagttc ctgtccaacc acttggaact
     1501 ccgctagaaa aattatacgt gtcgattggc gtataactta caccacttcc cccagaccgt
     1561 cgattcaatc caagtaaaac aaaaagatta gttaaaccag atgatccacc tccattcgga
     1621 gttaattcag aaactaacgc agcagtgcac ccctgcaaca aaacaaaact aacaacgatt
     1681 atccataact ttattttcat acctatctcc ttttttctgc ttctctgatt tctgtttcta
     1741 gaactttgat ttctgacatc acctcggcaa tatcagattc tcctgcgaat gcacaggcga
     1801 ttgtgatggt tgctttgaat gttctgcatg cagtttctac attgcttgcc acatcttgga
     1861 aggcggatgt gtttactcct gtgagagcac caaatacttg cgccccttgt cttcccaatc
     1921 ttgcattttg gatgcagtct tgtagacctt ctttggctcg tttgcattca gaaggattct
     1981 tctttccaga gcgcttcatc tctgcgagtt gtatctcttt ctcttctttt tcttgtcgaa
     2041 tcttttcaat tcgagcaagt ctatcagctt cctcctgacg ctttgcttcc tctgccgccc
     2101 atttttcatc acgctttctt gcccctggct cgtttgcatt tttccaactc actaggtctt
     2161 ttagcttttt gtctgtctcg gagataatga gccttatctg cttcttgtta cctggctccg
     2221 ctttctcaaa ctcttcttct agatattgtt tgcgacttgt atacttctcc gttagagttc
     2281 tgaacctaca atcaatgtat tccacttctg atccagggct tgaactgcaa gtaaacggtt
     2341 tcactagaat aggcgcttct aggtattctt tcttttgaac tacactaacg caattggtga
     2401 ggctaattag gagtatgctc attgtcactt tgagagctag acgagaagtg ctaatagaat
     2461 aaagcgattt cctacttgtg gttttcgact gtcgcttact ctgctcgaac tgacatttta
     2521 attttttcaa aatatttctc atacttcctc ctatttttta ccgagtgctg cgagtataat
     2581 ttcttctgtg gatttttcga ttgcttcgtt gaattcggat gaatttagat tggataaaat
     2641 aaacgtccga gtcgatttgc ctttcgtcac aggagtagat tgaaatttta cggatcctgt
     2701 ttttatatct accacagaga attctaaagt gtgatctatc ctccaagcac cagacaagtc
     2761 aatccaccag aggtctaaga ggagcggaaa ataaaaaata ttccctactc ccaaatcagg
     2821 aactctcgta ggataatcga gcgtaacctt tgacttaact tcgattcgtg cgttaactcc
     2881 tagtgtttct aaaatcctcg ctcggtcttt gtccgtttct gattcttcta caaaagaaat
     2941 tctatctgct gggacagctt caattctaga acttgcgtat tctgtaaagg ctgttgtaaa
     3001 actttttgaa taagacttct ctaatccatc cttgcttgcc tctgttaaga ctggcggact
     3061 ttccatagaa tcccgcatcg ttgccttttt catttccact tctatcttat ttggaaaaga
     3121 gagaacaaac tttcggattg attttaaata ggatccttct tcaacagagg gtgtgcgtat
     3181 ttcagctatg ctgcactggc agaaaatagg aaggattaga aataggtaaa tagatcgcct
     3241 atgtttcata acgattctcc tttggaactt aatacaccca ctctatacac cacaaaaaaa
     3301 cattgtcaag tatactggaa caaacgggca ttttttggga atgaacggta aatcaatatt
     3361 attcgaataa agttttcttt taaaaacagt ctggaagatt taacatccgt ttgccgcaac
     3421 ctctaagaga actttatcta tctaatcttt gtgataataa aacaatacat ctgaattcgt
     3481 tttcaaacta ccttcgttcc ataaaagcac actattttta ttaaaaaagt cacgttcata
     3541 ccaaagatta cttgtaatcc tagagacact agaaattgat aggataatga agagtgtatg
     3601 gaaagtttta gttatagtaa gttttagttt atctgcggga gagttaccgt ttgcgaaaaa
     3661 tacagttgta ttaggtgagg gagatacttg gatcaatatc atgagtaacc tcgaagagtt
     3721 acaggatacc tctacagata gaagtatcca ttatccgctc tctaagaaag aagactttaa
     3781 acaagttcca agcacaatga agatttggtc atcccctgat accaacattg aatctggcgt
     3841 gtggtttaga gtaaagcttc gaaatgaaaa tcccaatgaa aatatgtatg ttttgaatga
     3901 tacttatcag gcaaagctaa tattgttttt cctcttagat gaaaatggga aaattctatc
     3961 tgaaaagaaa gtcggatttg gaacggatca caaggattca tttcttaaat ttaattccaa
     4021 ttcaatttcc tttgatttaa aatacaaaga agaagtttat ctatatttgt ttctctatag
     4081 cgaaggattt caatttccaa atttcgatat ttttatatgg aaagaacaaa tttactttga
     4141 acactatcgc actatttatt tgtatcttta ctatgggatt gcaatttcca ttttctttta
     4201 caatttattt ttattttttt caacgaaaga taaagcttat ttgcattatt gtttttatgt
     4261 tctttcggtt atttgctttt tcttttgtta tcagcagcag gttatttttt taccttttgc
     4321 tatagtaaaa aattacggtg actattttat tcaaattttt gctttcttta caatcattac
     4381 aatgattaag tttgccctat cctttttaaa cttaaaatca atttataaga ttactttctt
     4441 cttattagaa tccgcagtaa agattttata tatttatcta atactcttac tatttttaag
     4501 aatcttttta aaatctgata actttataat aaacaatttc atgctcatta tgcagcctac
     4561 aatacttata atagatttat taattactta tcttgcaatc tcagcctggt ggaatggata
     4621 tattagagca aaatatttta ctatagcttg gattttatac ttatttggat tattttatgc
     4681 ttttcttatt aattttttga accttgcaaa tattcagcta ttcgcttcat ggggaattgg
     4741 attcttaatc ttaactcata tgaatatgtt tttaggttcg atagaaatgc tattactttc
     4801 ctttgcacta ggagatagat ataacttgat tcaacaagaa aacgagcgcg ccaaacaagc
     4861 gttacttgaa agagagattc aactaaagga agcagaagcg acaaagaaaa aattagaact
     4921 aggtatgctt aaacaaacaa tacaacccca ttttcttatg aattctctca acacagttct
     4981 tggactcatt cgagagaatc cagataaggc aggagaattt gtgagttttc tttgtatgga
     5041 gatacgcaca atgctagatg tatcgagtga gcggctaatc cctatttcct ctgagtttgc
     5101 tatctgtaaa tctcacttgg agattatgcg aattcgtctc gaacgaaaat tcacttggaa
     5161 gacacaagat atttccgaag aggaaaagat tccgccatta ttatttcaaa cgctccttga
     5221 aaatgcattt actcattcta aaggctatac tggcaatatt gcgttccttg taaaaaaatt
     5281 aatttgtgat cctgaaaagc catttcctag tggaaatata gaatggatac aggatcaaac
     5341 cctccaagct ttaaaaagaa ttgaatcagt gtatgaattc tattcaatct acgatgagtc
     5401 gtataaggct aataccaatt tatcgcaaag taactaccgc actggagaag atggaacggg
     5461 aactctctac attaaatctc gtctagaaga aagctatggt gataattgga gtttctcgca
     5521 aggctttgag ggaaaatctt gggttgttag aatcggtgtg ggaaaataaa tcaccgatag
     5581 tccctgatta atgcccgttc gttcccgact gcttgacacc aaaaaagaat gacgcatagt
     5641 taagaaaatt aaatttaggt cgctaaatga aaacagagaa aatcaagaaa aacagtgcaa
     5701 tcaaggtttt aatcatagaa gatgagccgg ttactgcgcg ttatattcaa actttacttc
     5761 gtgaatttta caaatctagg aacgttagta tttttatgca aaaaagtcta ataggatctc
     5821 aggcttttat tgaagaacaa agcattgact tgattttact tgatttgaat attaatggag
     5881 aggatggtct tgaaattatg gagagtccct ttatacaaag cattcaaacc attgtgattt
     5941 ctggaaatac ggagagagca atagacgttt tccgccattt agttttagat tttgtgccaa
     6001 agcctgtttt acccaaacgt ctctttgagg ctttagaaaa atatgaagca aaagaatctt
     6061 ggaagaaaaa agggaaacat ctttccatta aaaaagatga tatgattgag ctagtatcct
     6121 atgaaactat ttgttatttg aaaggagagg gcaaaacaac aaaagttttt ttaaaagagg
     6181 ggaatccgct tacaatcaat aaaaatatta attctgtttt aatgaacctc ccagattatt
     6241 tctttcgaat tcataaatcc tatgtgattc cactcaattg cataaataaa ataaaactaa
     6301 aaccggcttt cacagtaatt ctaaagaacg ggactgaact ccctgtagca aagagagtaa
     6361 taaaaaaatt acaagaagaa gttttgtagt aactgacgtt tattacgatt caatttgaac
     6421 catgttcaaa gttcacaatt tagattaggg atttgtaaga atttaaccac tccgataaac
     6481 gcaagataga cactggcaca gcgaaatgca ttgtgttgaa tcgaagtgat acagaatatt
     6541 ttttttgtgt caaaggttag tttcaaacat aatcccattt tttttatgta atagatttag
     6601 gcattggtga agttacctct cttactcgta aggaatcatt cgagaaaaag taaaattaat
     6661 tcttacttgg aattataaac gttttacagc aatttacccc gaaggcaaaa caataattcg
     6721 caaaccatag tcacacaatg atctaatctt tcctc
//
LOCUS       DAOQOS010000202         6705 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_6527_length_6705_cov_6.530226, whole genome shotgun sequence.
ACCESSION   DAOQOS010000202 DAOQOS010000000
VERSION     DAOQOS010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 6705)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6705)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6705
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6527_length_6705_cov_6.530226"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(53..940)
                     /locus_tag="PLF66_24830"
     CDS             complement(53..940)
                     /locus_tag="PLF66_24830"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HMZ67336.1"
                     /translation="MRNKIFIIDDSKSFSKLIALRIKNDLGLDSITASSFSEAKKVLE
                     EERDSIFLALVDLNIADAKGTEHIDYMLEEKVPVIVITGELNDEIRNKILEKNIIDYI
                     FKSKKEDMEYVIKLLRQIVRNSHTKVVVADDSPLFRKLTSDLLRLQMFQVLEAKNGLD
                     CLNIVHDNPDIKLVLTDYNMKEMDGFELTLRLREKYPKESLIVISISGNDTKNISSKF
                     IKIGANDFLTKPFTKEEFACRINMNLDALDSLEKIKDLSYRDFLTGLYNRKYFLEKGK
                     SILTTDKNAGLALFIVDHF"
     gene            complement(960..1928)
                     /gene="nadA"
                     /locus_tag="PLF66_24835"
     CDS             complement(960..1928)
                     /gene="nadA"
                     /locus_tag="PLF66_24835"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008593830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HMZ67337.1"
                     /translation="MKTEELVKHLEKTYTEFEIEKRLPLIEEINRLKKEKNAVLLGHN
                     YMTPDVFYGVSDIVGDSLYLSKMAAETTADMIVFNGVHFMAETAKILSPNKKVLIADL
                     KAGCSLAESITRQDVIELKKQYPGVPVVTYVNCSAEVKAETDVCCTSGNAIKIVNSID
                     SDTIIFLPDQYLASNVQKHTTKKLISHPGSCMVHELYTKEDIQNTRKLFDDLVVISHP
                     ECKSDVVDASDFAGSTAEMANFIKKSDAKNIMLITECSMGDNLRSEFPEKKFVSTCQT
                     CPHMKKITLEKIRDTLIYEQYEIHLEKEIIEKGQKAVRRMLELSYK"
     gene            complement(1935..2459)
                     /locus_tag="PLF66_24840"
     CDS             complement(1935..2459)
                     /locus_tag="PLF66_24840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67338.1"
                     /translation="MKNLKQKGIILVLLTLLQASCGGSDNTSKRQEQLQLLIYLRNNQ
                     SPTSSTQAACFNFISAENTCITLPDGQSVSCTDSEISRIKSGIQTSDKRTDSILIGFF
                     GCWKNCAILFNAANSICTTSKYPTTKSYREAQKSTSNTASVAWGSCMTTCNSGASEES
                     GLKGTGATYPTQPF"
     gene            2562..3317
                     /locus_tag="PLF66_24845"
     CDS             2562..3317
                     /locus_tag="PLF66_24845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67339.1"
                     /translation="MEKKHYKQIAIFLLLLMVTAGGLYYYFKVYKKNLEAPDYINMSD
                     SLFQRIKENPDYLIAENLLEIKSMPLLEYLRTRILFYRYSDPDNAKKRIQTELEMDFP
                     GERGRALIEILEVYLKLEKGIEEIEKEDTLDSYEKILKIELLKAELFGEKLMVLLFPR
                     KDSEAIERFYAYSERYLKKHYTDMPRSKKIHLGKARREIYGEEYDRIFAMEPMRRRFE
                     LELKINERELSILNEYEKKQAIEAIKEKLNQEE"
     gene            complement(3556..3870)
                     /gene="trxA"
                     /locus_tag="PLF66_24850"
     CDS             complement(3556..3870)
                     /gene="trxA"
                     /locus_tag="PLF66_24850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015681003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HMZ67340.1"
                     /translation="MNQDLPKSFFDLIATHDKPILVDFWAEWCGPCKMLSPVIQELAA
                     EWKGKVTVIKINTDDKPNIAGHYSISSIPTVILFKNGKEVKRISGAMPLQQLKNSFGP
                     FI"
     gene            complement(3878..6583)
                     /locus_tag="PLF66_24855"
     CDS             complement(3878..6583)
                     /locus_tag="PLF66_24855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000612751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HMZ67341.1"
                     /translation="MVQPSLTSLLQENQRGVASIESNQNLTDVFVSVLNEAIKNVGAK
                     TGYLFFIDEENNLKSFGSSSSSREERLIANYCFSSKKNLQIKKNGAIPESTITADQSY
                     IACYLGDDRTAIGVFLLAGINHFENFSTNDFELISVYSRTLSELLRDSLILPERGEVF
                     LHFTTAIQLLFHSTDNLQKNTRLEFLLKEVIRVSGLINSSRHLTNLLKDVMESVKSVF
                     RTESCSILLVDKVKNELYFHIVAGEKEEEIQKIRVPMGKGIAGTVAVTRVPMIINDAQ
                     NDDRVYKVVDKASDFTTRNIIATPLIVDDEVIGVMEGINTIDRNNFNDSDIELFLSFS
                     DAAAVAIQKTRLLDNLEKKVSELGSLFDLGKAVLESKDEEDLILKSIRIVVREMDAKH
                     AVILIIDPVKEKLNLWSYMNGLEEKSQETFPRDSIIVHAMIDNRILLRDSYYMQQDDD
                     ILDKKFLKNSFIVLPLVGGNQKPFGAICISDRVDQLHFNDNDLRLLQTISSQFIKGYE
                     NIKLNADMIAKKAMQKEIEITRNIQNNILPSKIPTGSNFELGVKSVPAKDVSGDFYDF
                     YKYEDGQFSFLIADVSGKSLPAAIFMAMSSSVMRTLSRNHEMNPADLLKAANSLIYED
                     SQSGMFVTLFFIHYDPSIMEIEFASAGHNDQIWIKADDSYEFIKGTGAPLGVIPTSKY
                     VGGKIHPKSGDMVVLYTDGAVEEKNKDNEEFGLERFVQEIIKRKEKHPQQIIEEVYTE
                     IVNFSESSEQFDDFTVLILKFNDDYQFRKTFPAVNASIPKFRDFVFETIKIRNLNEFF
                     CDDILLCCDEAATNVVMHSYKDTKLPNPTFDCGIKFTEEKIIIRIQDKGIPFERSKVP
                     APSLEANLKGERRGGFGVYLVEKLMDHVTYIHENGINTVIMEKKF"
BASE COUNT         2126 a         1121 c         1135 g         2323 t
ORIGIN      
        1 gtgagtttaa aattaaatct cctgcttctt gaccaaaatc agaatttagt ttttaaaaat
       61 gatctactat aaatagagca agaccagcgt ttttgtctgt ggttaatata gatttacctt
      121 tctctagaaa gtattttctg ttataaagac cagttaaaaa atctctataa gataggtctt
      181 taattttttc aagactgtct aacgcatcta aattcatatt gatacgacag gcaaattctt
      241 cctttgtaaa tggttttgtt aaaaaatcat tcgctcctat ttttataaat ttagaagaaa
      301 tattctttgt atcatttcca gaaatggaaa taacaatcaa agactctttt ggatattttt
      361 ctctgagtcg gagtgtaagt tcaaaaccat ccatttcttt catattgtaa tcggttagga
      421 ctaatttaat atctggatta tcatgaacta tattgaggca gtctagacca ttcttagctt
      481 ctaagacttg aaacatttga agtcttagca agtcacttgt taatttacga aataggggag
      541 agtcatctgc tacaacaacc tttgtatgac tgttgcgaac aatttgtctg agtaatttga
      601 ttacatactc catatcttct tttttggatt tgaatatata atctataatg tttttttcta
      661 aaattttgtt tctgatttca tcatttagtt caccagtgat tacaattaca ggaacttttt
      721 cctctagcat ataatctata tgttctgttc cctttgcatc tgctatattt agatccacta
      781 atgctaaaaa tatagaatct ctttcttctt ctaatacttt ctttgcttcc gaaaaagaac
      841 ttgcagtgat ggaatcaaga cctaaatcat ttttaattcg aagggcaatt aatttagaaa
      901 aagatttact gtcatcaata atgaatattt tatttctcat ggtataattg tgattatcct
      961 cacttatagg aaagttctag catgcgtcta actgcttttt gtcctttctc tataatttct
     1021 ttttctagat gaatttcata ttgttcatag ataagagtat ctctgatttt ttcgagtgta
     1081 atttttttca tatgtgggca ggtttgacat gtagagacaa acttcttttc agggaattct
     1141 gacctaagat tatcacccat tgagcattcg gtaatcaaca ttatattttt tgcatctgat
     1201 tttttaataa aattagccat ctctgctgta gaacctgcaa aatcagaagc gtccacaaca
     1261 tcgcttttac attctggatg agaaataaca actagatcat caaaaagttt tcgagtattt
     1321 tggatatctt ctttggtata gagttcgtga accatacaac ttccggggtg agaaattaat
     1381 tttttagttg tatgtttttg aacgttagat gctagatatt gatcaggtaa gaatataatc
     1441 gtatctgaat cgatagaatt cacaatttta attgcatttc cagatgtgca acatacatca
     1501 gtttctgctt tcacttcagc ggaacagttt acataggtaa caactggaac tcccggatat
     1561 tgttttttta gttcaatcac atcctgtcta gttatgcttt ctgcgagtga gcagcctgct
     1621 tttaaatctg caattaggac ttttttgttc ggagataaaa tcttagcagt ttccgccata
     1681 aaatgcacac cattaaagac aatcatatcg gctgtcgttt cggctgccat cttacttaga
     1741 tagagagaat caccaacaat atcagacacc ccataaaaaa cgtccggagt catataattg
     1801 tgaccaagta gtactgcatt tttttctttt ttgagtcgat tgatttcttc aattaaggga
     1861 agtcgttttt cgatctcaaa ttcggtatat gttttttcta aatgttttac tagttcttct
     1921 gttttcatag ttttttaaaa tggctgagta gggtaggttg ccccagtccc ctttaagcca
     1981 gattcctcgc ttgctccgct attacatgtt gtcatacaag atccccacgc cacagatgcc
     2041 gtattagaag tagacttttg tgcttctcgg taggacttcg tggttggata tttagaggtt
     2101 gtgcagattg aatttgctgc attaaataag atcgcacaat ttttccaaca tccaaagaat
     2161 ccaattagaa tagagtctgt tcttttatcg cttgtctgta taccactttt tattcgagag
     2221 atttctgaat cagtgcagga tacagattgt ccatccggaa gagtaataca ggtattttct
     2281 gcactgatga aattaaaaca agccgcttga gttgaagatg taggagattg attgtttctt
     2341 aggtaaatca gtaattgaag ctgttcttgt cttttggatg tattgtcaga gccaccgcag
     2401 cttgcttgaa gcagtgttag caataccaaa attatccctt tctgttttaa gtttttcatg
     2461 tagatatcat tttttttgat ttctattctt tgaaaagcaa gaattttacc agatttagat
     2521 agagacaagt gataagaaag tttcgataat taaagagatc tatggaaaag aaacactata
     2581 aacaaattgc tatatttttg ctcctcctca tggtaacagc gggaggtttg tattattatt
     2641 tcaaggtata taaaaaaaat ctagaagcac ctgattatat aaatatgtca gattctcttt
     2701 tccaaagaat aaaagaaaac ccagactact taattgcgga aaacctttta gaaattaaat
     2761 ctatgccgct tttagaatat ctgagaacta gaattctttt ttacagatac tcagatccag
     2821 acaacgcaaa aaaaagaatt caaactgagt tggaaatgga tttccccgga gagagaggaa
     2881 gggcattaat agaaatctta gaagtttacc taaaattaga aaagggaatc gaagaaatag
     2941 agaaagaaga tacgttagac tcatatgaaa agattctgaa gatagaactt ttaaaggcag
     3001 aactctttgg agaaaaatta atggttttac tttttccaag aaaagattct gaagccattg
     3061 agcgatttta tgcgtattct gaacgttatt taaaaaagca ttatactgat atgccaagat
     3121 ctaaaaaaat tcatttaggt aaggcaagac gtgaaatcta tggagaagag tatgatcgta
     3181 tctttgcaat ggagccaatg cgtcgtcgtt ttgagttgga actaaaaata aatgaacgag
     3241 aactcagtat tttaaatgaa tacgaaaaaa aacaagcaat tgaagcaatc aaagaaaaat
     3301 tgaaccaaga agaataaata ttaattttta taatatttgc taataatcct gaaccataaa
     3361 aatcaatagc ctacgaaaga gctagctaaa ggagagcgaa aaagcgatgt gtgaaaagct
     3421 ctccgcaggt agaaaacgaa tctaatgctg agagggtttg tgaaaaatag agaaaaagga
     3481 ttggtgactt ttagaatcca tacttttaat aaacccttcg acaatcttag ggcgacactt
     3541 ttacgcagtc tctaattaga taaaaggacc aaaggagttt tttaactgct gcaatggcat
     3601 agctcctgag attcttttaa cttcttttcc gttcttaaat agtataacgg ttggaatgct
     3661 tgagattgaa taatgccccg cgatatttgg cttatcatct gtattgattt ttataacagt
     3721 tacttttcct ttccattctg ctgcaagctc ttgaatgaca gggcttagca tcttacaagg
     3781 accacaccat tctgcccaaa agtctactaa gataggttta tcgtgtgtgg cgattaaatc
     3841 aaaaaaagat ttaggtaaat cttgattcat aagtgagtta aaattttttc tccatgatta
     3901 ctgtgtttat tccattttca tgaatatagg taacatgatc cattaatttt tccactagat
     3961 agactccaaa accacctctt ctttctcctt ttaaatttgc ttctaaggaa ggagcgggga
     4021 ctttggatct ttcgaaagga atacccttgt cctgaattcg aatgatgatt ttctcctctg
     4081 taaatttaat tccgcagtca aaagtagggt ttgggagttt agtatcttta taagaatgca
     4141 ttacaacgtt agttgctgct tcatcacaac aaagaagtat atcatcgcag aaaaattcat
     4201 ttagatttcg aatcttaatt gtttcaaaca caaaatctct aaacttagga attgatgcat
     4261 tgacagctgg gaatgttttt ctaaattgat aatcatcatt aaattttaaa attaaaacgg
     4321 taaagtcatc aaactgctct gaggattcag aaaaattgac tatttctgta tagacttctt
     4381 cgatgatttg ttgtggatgt ttttcctttc gttttataat ttcttgaaca aatctttcta
     4441 agccaaattc ttcattgtct ttattttttt cttctacagc accatcagtg tataaaacaa
     4501 ccatatcgcc agacttaggg tgaatctttc caccaacgta tttgcttgtt ggaataactc
     4561 ctagtggagc accagtccct tttataaatt catagctatc atctgccttg atccaaattt
     4621 gatcattgtg accagcggag gcaaattcta tctccatgat cgatggatca tagtggataa
     4681 aaaataaggt tacaaacatc cctgactgag aatcttcata aatcagagag tttgctgctt
     4741 ttaataaatc agctggattc atttcgtggt tacgactgag tgttcgcata acggatgaac
     4801 tcattgccat aaagatagca gcaggtaaac ttttaccaga cacgtctgca attagaaagg
     4861 aaaactgtcc atcttcgtat ttataaaaat cataaaagtc tccggataca tcttttgcgg
     4921 gaacagactt tacaccgagt tcaaaattag aacctgtcgg aattttagat ggtaaaatat
     4981 tgttttgaat attgcgggta atctcaattt ccttctgcat tgcttttttt gcaatcatgt
     5041 cagcattgag tttaatattc tcatatcctt taataaactg agaagaaatt gtttgtaaaa
     5101 gccttagatc attatcattg aagtgtagtt gatctacacg atcggatata caaatcgctc
     5161 caaatggctt ttgattgcct ccaaccaaag gaagaacaat aaaagaattt tttagaaatt
     5221 tcttatctag aatatcatca tcttgctgca tataatagct atctctgagc aagattcgat
     5281 tgtctatcat ggcatgaaca atgatagaat cgcgagggaa agtttcttgg gacttttctt
     5341 ctagtccatt catataagac caaagattta gtttttcttt aacagggtct ataattaaaa
     5401 taactgcatg ttttgcatcc atctctcgca caacgattcg gatggacttt aaaattagat
     5461 cttcttcatc ttttgattct aaaactgctt tccctaaatc aaataagctt cctagctcac
     5521 tcactttttt ttctaggtta tctaagagtc ttgttttttg aatggcgaca gcggctgcat
     5581 cggaaaagct taaaaatagt tcaatatcac tgtcgttaaa attatttcga tcaattgtgt
     5641 tgataccttc cattacaccg attacttcat cgtctacgat gagaggagtt gctataatat
     5701 tgcgagtagt aaagtcggag gctttatcga ctactttgta gactcgatca tcattttgag
     5761 cgtcatttat aatcatagga acgcgtgtaa cggctacagt tcccgcaatc ccctttccca
     5821 taggaactcg tattttttga atctcttctt ctttctcgcc agcaacaata tgaaaatata
     5881 attcgttttt tactttgtct actaatagaa tagagcagga ttctgtgcga aaaacacttt
     5941 ttacggattc catcacatct ttcaataagt ttgtaaggtg acgagatgag ttaattagac
     6001 cagatacacg gattacttct ttgagtaaaa attcaagacg agtatttttt tggagattat
     6061 cagtagaatg aaataataac tggatggcag ttgtaaaatg taaaaaaact tctcctcttt
     6121 cagggagaat cagggaatct ctgagtaact cacttaacgt tctagaataa acagaaatta
     6181 actcaaaatc atttgtagaa aaattttcaa agtgattgat acctgcgagt aagaaaactc
     6241 caatagcagt tctatcatca cctaagtaac aagcaatata agattgatct gcagtgatag
     6301 tagattccgg aattgctcca ttctttttta tttgtaagtt ctttttagag gaaaagcagt
     6361 aatttgcgat tagccgctcc tctctcgaag aagagctaga tccaaaactt tttagattat
     6421 tttcttcatc tataaaaaat aaatatcctg tcttggcacc gacgttttta atcgcttcat
     6481 ttaaaacaga aacgaaaaca tcggtaagat tttgattgct ttcgatagat gcaacgcccc
     6541 tttgattttc ttggagcaag gatgttaaag atggttgaac caatttagat taagagaagt
     6601 ttagtttgag agcttcttct ctgagctgtt tatttgctgt ttctaattct tttatttttt
     6661 cgagagcatt cattagttct tctcttgaaa tattggaaac aatgt
//
LOCUS       DAOQOS010000203         6690 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_6543_length_6690_cov_9.266315, whole genome shotgun sequence.
ACCESSION   DAOQOS010000203 DAOQOS010000000
VERSION     DAOQOS010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 6690)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6690)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6690
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6543_length_6690_cov_9.266315"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(422..712)
                     /locus_tag="PLF66_24860"
     CDS             complement(422..712)
                     /locus_tag="PLF66_24860"
                     /inference="COORDINATES: protein motif:HMM:NF013677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HMZ67342.1"
                     /translation="MREYPKEYKIESVKLYLANGRQKNKTARDLGVPLTTLRGWIKKY
                     MSEAIDKSKEKPKKRDYEKELKEKQKIIEQQQEEILILKKSIGIFTRDSQKK"
     gene            complement(899..2191)
                     /gene="mtaB"
                     /locus_tag="PLF66_24865"
     CDS             complement(899..2191)
                     /gene="mtaB"
                     /locus_tag="PLF66_24865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000555999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HMZ67343.1"
                     /translation="MKESTVAFHTLGCRLNIFESDGLANTFSNLGIKTVSESENPEIV
                     VINTCTVTNKADSKNRNIIRNAIKNNPGSQIWVTGCYAETDKETIEKIPGVTGVIGNT
                     EKSNLPYIILKNKGERLDIDTNGNRFSYSDVLPTNHTRAYLKIQDGCNRSCAYCKIPQ
                     ARGKGVSRNIKDVLDQVRFLQDNGVGEIILTGVNLGWFRDESNTKNFNALLKNILDIL
                     EYSRLRLSSIEPSDVGHELAELTLHPRFCNYLHVPLQSGSSNILKKMKRSYTAETFYK
                     RISLVKQKNPSIFIGTDIITGFPNESEEDFQESVKIVKDLEISKIHAFPYSQRRGTLA
                     ETFPDNISKDIKKERVKILNLLSQDQYKNFGLKLIDKKREAILESDGTCMTDDYLKVR
                     LESGKLGLTTGQFLNVKIKEYKEPTAELKEGHFLGEVC"
     gene            complement(2188..2943)
                     /locus_tag="PLF66_24870"
     CDS             complement(2188..2943)
                     /locus_tag="PLF66_24870"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HMZ67344.1"
                     /translation="MRILLTLLFISIAGLSAQSMTYEKALAEYKAASYQESLQTIREI
                     LKVEKPRYELHYLAAFDYWNLKEYKSAINHFQAAIRLKPDDTKAYIDIVKVYTSAQSY
                     KQALELCEDAIKRFPQEPKLRFQQVTLLLKYGRVDVALEIIEKLKLTLPEDYRPLALE
                     SNIYYMKRDFEKAEISLKWASALSPSNANLKNNLALIYEQMAIINLKTNEKEKAKVNL
                     DLAKSQLESAISTNSNPVFLENQKRIVSLSNSL"
     gene            complement(3000..3434)
                     /locus_tag="PLF66_24875"
     CDS             complement(3000..3434)
                     /locus_tag="PLF66_24875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67345.1"
                     /translation="MIHIVIPVLVVLSYFLFRNLWFELRKNRILASIEKISLGIIEPG
                     IILPEVSIRYKYYYEEGVYYGTGYLLLQDFLYPYEYRAGFTQEGVPYIAIGDKSFLSE
                     EHIETFLINQCELVSIFIDPVEPFHSELESIYTESIGVEELK"
     gene            complement(3431..5953)
                     /locus_tag="PLF66_24880"
     CDS             complement(3431..5953)
                     /locus_tag="PLF66_24880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004767629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HMZ67346.1"
                     /translation="MVEFTKRAKRVINEMAGEEAKRLGHDFVGPEHIFLGLLREEDSV
                     AVKILINLNINLNELKREVEKKSREDNLLLDLPASQDRYQRIVESSKEEAKRMKHNYV
                     GTEHILLALLRDNSNIAAAVLSTFGVNYNVIKSEILRLLGVPPVGTVGVSSGQQSAQQ
                     SSTQQRGEKSKTPILDEFARDLTQLARDKKLDPVIGRAKEIERVIQILSRKTKNNPVL
                     IGESGVGKTAIVEGLAIAIVEKTVPELLYEKRVLSLDLAGLIAGTKYRGEFEERLKKI
                     MKEITSSTSIIIFIDELHTLIGAGAAEGAVDAANILKPALARGELQCIGATTNTEYRK
                     YIEKDSALERRFQTVKVAEPSVDDAIKILDGLKKAYESHHKVRYTPKALEQAVKLSHR
                     YINDRYLPDKAIDIIDEAGSKARLANCGRPENIKEAEEEIRVLSVKKEELVRSQEYEK
                     AAAIRDEVNRKKLVVEDMIRAWQERLETQAVTIDEDQILSVISMWTGIPLEKMEESEN
                     EKLISMEKILRERVVGQNTAIETIARAVRRSRTGFKNERRPTGSFIFLGPTGVGKTEL
                     AKALATFLFGKEDHLLRIDMSEYMEPHAVSRLIGAPPGYVGYDDGGQLTEFVRRKPYS
                     VILLDEIEKAHHDLFNILLQIMEEGNLTDTKGRKVNFRDTILIMTSNIGAKEIQKGGR
                     VGFEDLEGEKDKAKSELARDELKKYFNPEFLNRVDEVIYFQPLTKEEIVAIVDIMLKD
                     FNARLLEKKIMVSMTLEAKEYMSEIGYDSAFGARPLRRIFQKELEDYMATQLLTGAYK
                     MPTIIKVDAKEKKFNFEEQPWEDYGIDTVGSDSDTKEVVKTN"
     gene            6413..>6690
                     /locus_tag="PLF66_24885"
     CDS             6413..>6690
                     /locus_tag="PLF66_24885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67347.1"
                     /translation="MKYKSLLQIGILLFLITCKIDKAGFHPIPEFNAETNARLESFFK
                     ETKDYPKRKVAVFDGDGTVLGQAPHYMADECLYEVAKQKPEKKPDVIK"
BASE COUNT         1888 a         1197 c         1134 g         2471 t
ORIGIN      
        1 atcaaaagcc tgatatcgtt agatgtcggg cttttttgtt tttgtgctgg tgtgacgttt
       61 agcattccag tttgctgtgg gagagtagga agggcgaagg cgttggtgct ccgaccaagg
      121 agcagggtgg actagatatt ttttgctatc atatcaaaag cctgatatcg ttagatgtcg
      181 ggcttttttg tttttgtgct ggtgtgacgt ttagcattcc agtttgctgt gggagagtag
      241 gaagggcgaa ggcgttggtg ctccgaccaa ggagcagggt ggactagata ttttttgcta
      301 ttggaactac atttcatttg tatgtaacca gctataataa ccactctttg aaacgctctg
      361 acctcacaca tttttgccac attaaaatta tctttatttt cttttaagaa attaaattaa
      421 tttatttttt ttgcgagtct cttgtaaaaa tgcctattga cttttttaaa attaatatct
      481 cctcttgctg ttgttctatt attttctgtt tctcttttag ttctttttcg taatcgcgtt
      541 tcttcggttt ttcttttgat ttatctattg cttcactcat atactttttg atccatcctc
      601 tcagtgttgt taatgggact cctagatccc ttgctgtctt atttttctgt ctaccatttg
      661 ctagatatag ttttactgat tctattttgt attctttggg atattctctc attttagtct
      721 cgttttggaa acctaaattt gtgcgcaggg cttcaactta cggttagcaa gcaaagcacc
      781 taaatccctc aaaaaagtca cagtcttaac aatggattct ccaaaaacaa aaaatccatc
      841 ccctttcgca aaggcgatta tcagtattat gctaagcaat ttctgaaaag tcaggggatt
      901 aacaaacttc ccctaagaaa tgtccttctt ttaattcagc ggttggttcc ttgtattctt
      961 ttattttaac atttaaaaat tgtcctgtgg ttaatcctaa tttgccgctt tctagtctaa
     1021 cttttaaata atcatcggtc atgcaagttc catctgattc taaaatggct tctctctttt
     1081 tatcaatcag ttttaatcca aagtttttat attggtcttg agagagtagg ttaagaattt
     1141 tgactctttc ctttttaata tcttttgaaa tgttatctgg aaatgtttct gccaatgttc
     1201 ctcttcgttg tgaataagga aatgcatgta ttttagagat ttccaaatct ttcactattt
     1261 tcacagactc ttgaaagtcc tcttctgatt cattcggaaa tcctgttata atatctgttc
     1321 caataaagat agagggattc ttttgtttta ctaaactaat tcttttatag aaagtctcag
     1381 ctgtataact tcttttcatt ttttttaaaa tatttgaact accactttgc aatggaacat
     1441 gaaggtaatt acaaaaacga ggatgaagtg ttagttctgc caattcatgc ccaacatcag
     1501 acggttcaat tgaggagagt cttagcctag aatattctag aatatctaaa atgtttttta
     1561 aaagtgcatt aaaatttttt gtattggatt catctctaaa ccaacctagg tttacaccag
     1621 ttaaaataat ttcaccgact ccattatcct gtaaaaagcg aacttgatct aatacatctt
     1681 ttatatttct actaacgcct tttcctctag cctgtggaat tttacaataa gcacaagaac
     1741 ggttacaacc atcctgaatc tttaaatatg ccctagtgtg atttgttggc aatacatctg
     1801 agtaagaaaa tcgatttcca tttgtatcta tatctaatct ttcaccttta tttttaagaa
     1861 taatataagg aagatttgac ttttcggtat ttccaataac tcctgttaca ccaggaattt
     1921 tttcgattgt ttctttgtct gtttccgcat aacatcctgt aacccagatc tgtgatcctg
     1981 gattgttttt tatagcattt cgaatgatat ttctattttt tgaatctgct ttatttgtaa
     2041 cggtgcaagt attgatgact acaatttctg gattttctga ttcagaaact gtttttatcc
     2101 caagatttga aaaagtgttc gctaaaccat ccgattcaaa gatgttgagt ctacatccta
     2161 acgtatgaaa agctactgtt gattctttca taaactattt gataaagaaa ctatcctttt
     2221 ttgattttct aaaaaaacag gattagaatt tgtgcttatt gcggattcta attgagattt
     2281 agctaagtct agatttacct ttgccttttc tttttcattt gtttttaaat ttataatcgc
     2341 catttgctca tagatgagtg ctaagttgtt ttttaaattt gcattactag gtgagagtgc
     2401 ggaagcccat tttaaactga tttctgcttt ttcaaaatca cgtttcatgt aatagatatt
     2461 agattcaaga gctaagggac gatagtcttc aggtaacgtt aattttaatt tttcgattat
     2521 ctctaatgca acatcaactc tcccatattt taaaagaagt gtaacttgtt gaaaacgtaa
     2581 ctttggttct tgcggaaaac gcttaatcgc atcttcacaa agttctaaag cttgtttgta
     2641 agattgagcg gaagtataaa ctttcacgat gtcaatatat gcttttgtat catctggttt
     2701 taatcgaatg gcagcttgaa aatgattgat tgcagatttg tattctttta aattccaata
     2761 gtcaaatgca gctaaatagt gtaattcata ccttggcttt tctactttta aaatttcgcg
     2821 aattgtttga agagattctt ggtaagatgc tgctttgtat tctgccaatg ccttttcata
     2881 agtcatggac tgcgcagata atcctgcaat ggaaatgaat aataatgtta gtaaaattct
     2941 catatcgaat atgcctttac tgacattttt tgaatgagcc cgaaataggg aacgaatttt
     3001 tattttaact cttctacacc tattgattca gtgtaaatcg attctaattc agaatggaat
     3061 ggctctactg gatctatgaa tatggaaaca agttcacatt ggtttattag gaaagtttca
     3121 atgtgttcct cggaaagaaa ggatttatct ccaatggcta tatagggaac accttcttgt
     3181 gtaaatcctg ccctgtattc ataggggtat aaaaaatctt ggagaagtaa gtatccagtc
     3241 ccatagtaga ctccttcttc ataataatac ttgtagcgaa tggatacttc aggtaatata
     3301 ataccaggct caattatgcc taatgatatt ttctctattg aggctaggat tcgattcttt
     3361 cgaagttcga accataggtt cctaaataaa aaataggaaa gcacaactaa gactggtatt
     3421 actatatgga tcaatttgtt ttaaccactt cctttgtgtc agagtcagaa ccaactgtat
     3481 caattccata gtcttcccaa ggctgttctt caaagttaaa cttcttctct tttgcatcta
     3541 ctttgatgat cgttggcatt ttgtaagcac cagtgagaag ttgggtagcc atatagtctt
     3601 ccaattcttt ctggaatatt ctacggagtg gtcttgctcc aaaagcagaa tcatatccta
     3661 tttcggacat atattctttt gcctctagtg tcatcgaaac cataattttc ttttctaaaa
     3721 gtctagcatt gaagtctttt agcatgatat ctacaatcgc aacaatttct tcctttgtta
     3781 atggttggaa atagatgact tcatctacac gattgagaaa ttctggattg aaatattttt
     3841 tcaactcatc tctagctagt tccgatttag ccttgtcttt ttctccctct agatcttcaa
     3901 aacctacacg accacctttt tgaatttctt ttgcaccgat atttgatgtc atgataagaa
     3961 ttgtatctct gaaatttact ttgcgacctt ttgtatctgt taaatttcct tcttccatga
     4021 tttgaagaag tatattaaaa agatcatgat gcgctttttc gatttcatct aaaagaatca
     4081 cagagtaggg ttttctgcga acaaactcag tgagttgtcc accatcatca tatcccacat
     4141 atcctggagg tgctccaatg agtcttgaaa cagcatgtgg ctccatatat tcagacatat
     4201 caattcggag aagatgatct tcttttccaa ataagaaggt tgctaatgct tttgctaatt
     4261 ctgtttttcc cactccagta ggtccaagga aaataaaaga accagttggt ctgcgttcat
     4321 ttttaaaacc agttcgtgag cgtctaacgg ctctagcgat ggtttcaatt gcagtgtttt
     4381 gtccaaccac tctctctcgt agtatttttt ccatactgat gagtttttca ttttccgatt
     4441 cttccatttt ttcgagtgga attcctgtcc acatagaaat gacggaaaga atttgatctt
     4501 catcaatcgt aactgcttgt gtttcaagtc tctcttgcca agcacggatc atatcttcta
     4561 caacaagctt tttgcggttt acttcatctc gaatcgcagc agctttttca tattcttgag
     4621 agcgaacgag ttcttctttt ttaacagata atactctgat ttcttcttct gcttctttga
     4681 tattttctgg tctgccacaa tttgcaagtc tagccttaga acctgcttca tcgatgatat
     4741 caatcgcttt gtctggtaaa tagcgatcat ttatgtatct atgagataac ttgacagctt
     4801 gttctaatgc ttttggagta taacgaactt tgtggtgcga ttcataggct tttttgagtc
     4861 cgtctaaaat cttgattgcg tcatcaacag aaggctctgc tactttaaca gtttgaaatc
     4921 ttctttcgag tgcagaatct ttctcgatat attttcggta ttcggtatta gtagttgccc
     4981 caatgcattg tagttcccct cttgcgagtg ctggtttaag aatattggct gcatctacag
     5041 ctccttctgc cgctcccgct ccaatgagag tgtgtaactc atctataaag atgatgatac
     5101 ttgtggagga agtgatctct ttcatgattt ttttgagacg ttcttcgaat tcacctctgt
     5161 attttgttcc agcgatgaga cctgctaaat cgagtgaaag aacacgtttc tcataaagaa
     5221 gttcaggaac tgttttttct actatcgcga tcgcaagtcc ttctacgatt gctgtttttc
     5281 ctacaccaga ttcaccgata agaactggat tgtttttagt tttacgagat aagatttgaa
     5341 tgactcgctc aatttctttt gcacgtccaa taaccggatc taattttttg tctcgagcga
     5401 gttgagtgag gtcacgagca aattcatcta aaattggagt tttgctcttt tcccctcttt
     5461 gctgagtgga agactgctgt gctgactgtt gtccggagct aacccctaca gttccaacgg
     5521 gtggaacccc gagaagtctt aagatctcac ttttgattac attgtaattt acgccaaaag
     5581 tagaaagaac tgcagctgca atattgctat tgtctcggag gagtgcgagt aaaatatgct
     5641 cggttcctac ataattgtgc ttcattctct ttgcttcttc ttttgatgat tctacgattc
     5701 tttgatagcg atcttgacta gctggtaaat ccagtaagag gttgtcttct ctagattttt
     5761 tttctacttc tcgttttagt tcatttaaat taatattaag attgataaga atttttacag
     5821 ctacggaatc ttcttctctc agtagtccga gaaaaatatg ctcgggacct acaaaatcat
     5881 gacccaaacg tttggcttct tcgccagcca tttcattgat tactcttttt gccctctttg
     5941 tgaattctac catatttaat cctttttaga agattgctta atcagactca atccattgat
     6001 gctaaataag ctttttttta cagacatcta caatttaaga tatgttgcat aagaaaatta
     6061 gcaagaaata atttcaaaaa tgtgaaatta ttgtatttaa aaaattttca aatgaaacaa
     6121 ttttagttac atttgtctat agtttattag acttaaaagt atgcaaatgg atttttttat
     6181 tttttgaatc ctatttttta atgtttaaat tgatcattca aataaaagcg acttcgattt
     6241 aaaaagaccc actcaaattc attttacaaa aagaggaaaa tgatatgccc tgtcgagtgg
     6301 atgctgacgt taatttagtc tttccagcaa aggaaatgaa tcaatttctt tacaggttca
     6361 attggaaaat aaatcgtatc caaaatacaa tatgttagga atggaaccat ctatgaaata
     6421 taaatctttg ttacaaattg gaattttgtt atttttaatt acttgcaaga ttgataaagc
     6481 tggctttcat ccaattccag aattcaatgc agagacaaat gctcgactgg aatctttttt
     6541 taaagaaacg aaagattacc caaagagaaa agttgctgta ttcgacggag atggaacagt
     6601 tttaggtcaa gctcctcatt atatggcgga tgaatgtctt tatgaagtgg cgaaacaaaa
     6661 gccagaaaag aaaccagatg taataaaaaa
//
LOCUS       DAOQOS010000204         6632 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_6628_length_6632_cov_5.857534, whole genome shotgun sequence.
ACCESSION   DAOQOS010000204 DAOQOS010000000
VERSION     DAOQOS010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 6632)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6632)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6632
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6628_length_6632_cov_5.857534"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..661)
                     /locus_tag="PLF66_24890"
     CDS             complement(<1..661)
                     /locus_tag="PLF66_24890"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015675833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HMZ67348.1"
                     /translation="MLEGNDIYRIGLDARPLSTRVSGVGRLIAETIRHFPDKKNFQFV
                     FFSHLPIHESHKSILDLPNIEFHQGKGFLSKKGATYFNLALPFSLHKYNLDLFWGSQQ
                     VIPPLPFFKKPIVLTYCDLVLYKYPETMRKIAALQQRLVQSYSVKKANFILGISEQTR
                     TDLVKHFKYPLNQTGVAYPGVDVKQALELAKQEPSDSIRNLKKPFLLSVSTIEPRKNY
                     PF"
     gene            complement(648..902)
                     /locus_tag="PLF66_24895"
     CDS             complement(648..902)
                     /locus_tag="PLF66_24895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67349.1"
                     /translation="MTQVIITQILGTVEITEGGKKITAKKGSKISIDAKIKTGSNGKV
                     ELEADGKTFLIQNDSNVNVKDIIKADGGNSGTTVAAGVRG"
     gene            complement(899..2278)
                     /gene="miaB"
                     /locus_tag="PLF66_24900"
     CDS             complement(899..2278)
                     /gene="miaB"
                     /locus_tag="PLF66_24900"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012388799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HMZ67350.1"
                     /translation="MQTIPKTEITESDLVEATQGLGKVYIETYGCQMNEYDSGIIKSL
                     MNKAEYTNTEDVESSDIIFLNTCAVRENAHAKIYSRLQNLGYLKKRNPNLVIGVLGCM
                     AQNLGDDLFHQELPLDLVVGPDNYRELPSLIQKIRSGEKKVNLTRLSKIETYDEIEPQ
                     VINGIQAFVTIMRGCNNFCTFCVVPYTRGRERSRDTASIVDEVKKLVDKGIKQVTLLG
                     QNVNSYRQEEKDFAYLVERILKETAIERIRFTSPHPKDFPLHLLKLMASEKRFSSNIH
                     LPLQSGNTRVLEAMKRTYSKEEFLQVVDEIRNTVPDCGITTDIIVGFPTETKEEFLDT
                     LEVVKKVQFDMAFMFKYSEREGTVAKRLLKDDVSEEEKADRLTELVELQTKISDYQNK
                     LKIGKTYEVLVEDRSRKSERELSGRTDSGRMVVAPIPENQTIEDFIGKMINIKIESAT
                     SATLKGKIV"
     gene            2415..2648
                     /locus_tag="PLF66_24905"
     CDS             2415..2648
                     /locus_tag="PLF66_24905"
                     /inference="COORDINATES: protein motif:HMM:NF015630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0175 family protein"
                     /protein_id="HMZ67351.1"
                     /translation="MAFIIEDSILAQLQFSETELMLELSLFLYQHNKLTLSKDCKLAN
                     VDRIEFQKNLLDRQIPIHYSIPDFEQDLKTIET"
     gene            complement(2663..3202)
                     /locus_tag="PLF66_24910"
     CDS             complement(2663..3202)
                     /locus_tag="PLF66_24910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67352.1"
                     /translation="MERFIDFGKRVKDLEITQMYGTIPEDNVKKMVKGFQANVGESGL
                     NELEFLFDFSDPEKLKIKKFEVDYWIVGWHGEPKPNVKEDWKTYLTAIPSIFTLFTIP
                     TFNETDIESNFLVYDKDFNLIKSFNYKNEVIVMISWLAGIPTKYKVWNKSGNIYEIAY
                     EPDLKEFSKDLARLIKDQK"
     gene            3891..4211
                     /locus_tag="PLF66_24915"
     CDS             3891..4211
                     /locus_tag="PLF66_24915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008589322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2834 domain-containing protein"
                     /protein_id="HMZ67353.1"
                     /translation="MTSFFKTVLFILGLGFTLSFCVIVIPPFLSNPDIIEAFAAGFVN
                     PYSSGYSLDTIFCWMILAAWIFYEAKTKHIKYGWIAALIGVIPGVATGFAFYLFLRMK
                     QNKD"
     gene            4430..4780
                     /locus_tag="PLF66_24920"
     CDS             4430..4780
                     /locus_tag="PLF66_24920"
                     /inference="COORDINATES: protein motif:HMM:NF013035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine rich repeat-containing protein"
                     /protein_id="HMZ67354.1"
                     /translation="MRKLFKNLFTLLFIFIPTVSLLPDCKVDQDKFCKDIPSPKAKIT
                     QCLLEHTNDLSESCKANLKDYVEKMRTQASGACKEDVNQFCKWVIPGGGRILKCLFKN
                     ESSLSASCKKILNE"
     gene            4868..6079
                     /gene="corA"
                     /locus_tag="PLF66_24925"
     CDS             4868..6079
                     /gene="corA"
                     /locus_tag="PLF66_24925"
                     /inference="COORDINATES: protein motif:HMM:TIGR00383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HMZ67355.1"
                     /translation="MRWKLARKKIEETVIDPFRPENIIQALKNPDEVLLKSLRSLTEM
                     TGSITGISFKSYSKLKSEAKGKSEFTFIGEKKVEEVKLQLFQYNEKEFTETKEITDYE
                     LVDSQNQSSNYWLNLHGIHDVKLIESIAQKLNLENLTVRQVIDTTQRPKMEDYDHYIF
                     FSIKSILLDELDRLDIEQLSFVLSKNYLVSFQEKVGDHFNFIRQRMRDNLGLVRKKEC
                     DFLLFQLLDAILDNYFETLDAINQEVGLIEKQTLTNPKQSTLLLIEKNKKNVSKIKKS
                     LAPFKEALTNILQDRSHFINEGNQKYFRDLTNSCSNAIEEANATYSALESLTNIYFSS
                     LSQKMNETMKVLTTVATIFIPLTFIVGVYGMNFENMPELKWQYGYFGVWGIMITTLFG
                     MIFYFKKKKWL"
     gene            6248..6616
                     /locus_tag="PLF66_24930"
     CDS             6248..6616
                     /locus_tag="PLF66_24930"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HMZ67356.1"
                     /translation="MNTEKYILLVEDEPINSHLAKIQLESKNYKVKEVATGEAAIELV
                     LNLKASFDLILMDIDLGPGMDGTQTAEKILSKINIPILFYSSHTEPEIIEKTEKITSY
                     GICGKKFRNHNLRRFYQNGS"
BASE COUNT         2188 a         1089 c         1077 g         2278 t
ORIGIN      
        1 ggaaaggata attctttcta ggttctattg tagaaacgga aagtaaaaat ggttttttta
       61 ggtttcgtat agaatcggag ggttcttgct ttgctagctc caaagcttgt tttacatcta
      121 cccctggata cgcaacaccg gtttgattaa gaggatactt aaaatgtttc actaaatctg
      181 ttctagtttg ttcagatatt cctagaataa aatttgcttt tttaacggaa taactttgca
      241 caagcctttg ttgcaaggct gcaatttttc tcatagtctc aggatattta tataaaacta
      301 aatcgcaata tgttaaaacg attggttttt taaaaaaagg aagagggggg ataacttgtt
      361 gcgatcccca aaacaaatct aaattgtatt tgtgtaagga aaaaggaaga gctagattaa
      421 aataagttgc gccttttttt gaaagaaatc ccttgccttg atgaaattca atattaggaa
      481 gatctaatat acttttatga gattcatgta tgggtaaatg gctaaagaaa acaaactgaa
      541 agtttttttt atcggggaaa tgacgaattg tttctgcaat taatctaccg acgcccgaca
      601 cacgggtaga taaaggtctt gcatctaaac cgattctata gatatcatta ccctctaaca
      661 ccagctgcta ctgttgttcc agaattgccg ccatctgctt tgatgatatc tttcacattc
      721 acattgctat cattttggat taaaaaggtt ttaccatctg cctctagttc cactttacca
      781 ttggagccag ttttaatttt tgcatcaatg gaaattttag atcctttctt tgcagttatt
      841 ttttttccgc cttctgtgat ttcaacggtt cctagaattt gagtaatgat tacttgtgtc
      901 atacaatttt tccttttaaa gtcgcactgg ttgcagactc tattttaata tttatcattt
      961 taccgatgaa gtcttctatt gtttggtttt ctggaatcgg agcgactacc attctgcctg
     1021 agtccgttct tcctgataat tctctttccg attttctaga tctatcttct actaaaactt
     1081 cataggtttt tccgattttt aatttgtttt ggtaatcaga aattttggtt tgcagttcta
     1141 caagttctgt aagacggtct gctttttctt cttccgagac atcatctttt aaaagacgtt
     1201 ttgctactgt tccttctcgt tctgaatatt taaacataaa tgccatatca aattgaactt
     1261 ttttgactac ttctaatgta tctaaaaatt cttcttttgt ttcggtagga aatccaacga
     1321 ttatatcggt tgtgattcca cagtctggaa ccgtatttct aatttcatcc acaacttgca
     1381 aaaattcttc tttggagtag gttcgcttca ttgcttctag aactcttgtg tttcctgatt
     1441 gaagtggcaa atgaatgtta gatgaaaagc gtttttcaga tgccatgagt ttgagtaaat
     1501 gcaaaggaaa atcttttgga tgaggagagg taaaacgaat tctttcaatt gcagtttctt
     1561 ttaagatacg ctctacaaga tatgcaaaat ctttttcttc ctgtcgatag gaatttacat
     1621 tttgaccaag gagtgtaact tgtttgattc ctttatcgac taacttcttt acttcatcca
     1681 caatcgaagc tgtatctcta cttctttctc ttccacgtgt gtaaggaacc acacagaatg
     1741 tgcagaaatt attgcaacct ctcatgatag tgacaaatgc ttgtatgccg ttgattactt
     1801 gtggttcaat ttcatcatac gtttcaattt tagaaagtct tgtcagattt acttttttct
     1861 cgccacttcg aattttttga atgagagagg gaagttctct ataattatct ggacccacaa
     1921 ctaaatcgag tggaagttct tggtgaaata aatcatcccc taaattttga gccatacaac
     1981 ccaaaactcc aatgactaag tttgggtttc tcttttttag ataacctaga ttttgcagac
     2041 gggaataaat cttagcatgc gcattttctc ggacggcaca ggtgtttaaa aaaataatat
     2101 ccgaactttc aacatcttct gtattcgtat actctgcttt attcataaga ctctttatga
     2161 ttccagaatc atattcgttc atctggcatc cataggtctc aatataaact tttcctagcc
     2221 cctgtgttgc ttctactaag tcggattcgg taatttctgt cttgggaatt gtctgcataa
     2281 ttttaaaaat tcaaaatgag tctctttgta aagtagaaat taatccgtat gggaatggaa
     2341 agggcggtta cgcgaccgtg caagaaagaa aatcatttga gttttagtaa ttctaatcta
     2401 tgggaaataa tttcatggca tttataattg aagattctat tctagctcag cttcaattct
     2461 cagagacaga attgatgcta gaactttctc ttttcctata ccagcacaat aaacttaccc
     2521 ttagtaaaga ttgcaaactt gccaatgtag atagaatcga atttcaaaag aatcttcttg
     2581 atagacaaat tcctattcat tattctattc ctgattttga gcaggactta aagacaatag
     2641 aaacgtaaat gaatagcttt tttcattttt gatcttttat caatcgtgct aaatccttcg
     2701 agaattcttt gagatctggc tcgtaagcaa tttcataaat atttccagac ttattccaaa
     2761 ctttatattt agtaggtatt cccgctaacc aagaaatcat cacgattact tcattcttat
     2821 agttgaagga tttaattaag ttaaaatctt tatcgtaaac taggaaattg gattcaatgt
     2881 ccgtttcgtt aaatgtagga attgtgaaaa gagtaaatat cgaaggaatc gcagttaaat
     2941 atgttttcca gtcctcttta acgttaggct ttggctctcc atgccagcca acaatccagt
     3001 agtcaacttc aaattttttt atttttaatt tttccggatc agaaaagtca aataaaaact
     3061 ctaattcatt taatcccgat tcaccaacat ttgcttgaaa accttttacc attttcttaa
     3121 cattgtcttc cggtattgtt ccatacattt gagttatttc caaatctttc actcttttac
     3181 caaaatcaat aaatcgctcc attggcttgt cggtgtctaa ttacgtacta attcgcattc
     3241 gtgatccctt ataaccttgc tgaataataa cgaattgctt tactctgaat tcattgtttt
     3301 taacatcttg tataaaattt tttatccgca cagtatttac attttctcga ctatataaat
     3361 cgggtggatt aaaataacaa ctcatgcaat ctatattgct tataaaagga tagaaaccaa
     3421 taagtccaat ttttttattt tttaaatgga attcttcttc caaactaagt ttgtatgctt
     3481 gagaatctcg tacaaagtaa tgccgacaat ttagaaagtt aaagagaata atcaataata
     3541 ctaatttcat ttttactcct taaaaggttt agctaactta aactctttat ttattggcaa
     3601 taatggaata aaaaaaacta tttgtctatg ggtattttca gtaaaaggat aactacctat
     3661 acgctttcaa ttcaaaaatt tccccatcta tttcaaaata tctgattcta aaacacccgc
     3721 aatcctttta tactttaaag ttttcgcttg tagtatagaa taggtaacga ttatggaaaa
     3781 agttggatgg aaattaggcg ctagccgcta gagtgaaaat tattttcatg caatagtcat
     3841 tcgtctatgt taatccttgc aaaacttcaa aacaatgcaa aacgtgattt atgacttctt
     3901 tctttaaaac tgttcttttc attttaggtt taggatttac actttcattt tgcgtgattg
     3961 ttattcctcc ttttctaagt aatccagata taattgaggc atttgcagca ggatttgtaa
     4021 atccgtattc ttcaggttac tcattagata caatattttg ctggatgatt ttagcggctt
     4081 ggatttttta tgaagccaaa actaagcata ttaagtatgg ttggattgct gcattgattg
     4141 gagtcatacc aggagtagct actggatttg ctttctattt attccttcgt atgaagcaaa
     4201 acaaggattg aaaataataa atgatagtaa ttagcttggt tatacagtta atgctcatta
     4261 accgctattt attttgtagg tgaagccact ttacaaagct tatatagtga tttgtattaa
     4321 aatgaaaatt ttaccccatc gaaattagaa tactttttat gaaagacttg caatcgaaat
     4381 taattcaacc tggattcaat tttaaattta aattctggag tcaatagcca tgagaaaatt
     4441 atttaaaaat ttattcacct tactttttat cttcattcca acagtttcat tattgccgga
     4501 ttgcaaagta gaccaagata aattctgcaa agacattcct tccccaaaag caaaaattac
     4561 ccaatgccta ctagagcata ctaacgattt gtcggaatca tgcaaagcaa atctaaaaga
     4621 ttacgtagaa aaaatgagaa cacaggcgag tggggcatgc aaagaagatg tgaatcaatt
     4681 ttgcaaatgg gtcattcccg gtggaggaag aattttaaaa tgtcttttta aaaatgagtc
     4741 atctctttct gcatcatgca aaaaaatatt aaatgaataa ttctgaatag aaattaccca
     4801 tccattttta tcttgcctag aaggctggat tttttaaatc tgaaaatgga agtattggta
     4861 acaaatcatg agatggaaac tagcacgcaa aaaaattgaa gaaactgtaa ttgatccatt
     4921 tagaccggaa aacattattc aagctctgaa aaatccagac gaggttttac taaaaagtct
     4981 gcgctcttta acagaaatga ccggaagcat aacgggcatt agctttaagt cttattctaa
     5041 actgaaaagt gaggcaaaag gcaaatcaga atttacattc ataggtgaga aaaaagtaga
     5101 ggaagtaaaa ctacaacttt tccaatacaa tgagaaagaa tttacggaaa caaaagaaat
     5161 cacagattat gagttagtcg acagtcaaaa tcaatcaagt aattactggt taaatttaca
     5221 tggaattcat gatgtcaaac tgatagagtc cattgcgcaa aaattaaatc ttgaaaacct
     5281 aacggttaga caagtaatag ataccactca acgccctaag atggaggatt acgatcatta
     5341 tatttttttc agtatcaaat cgattctatt agatgaattg gaccgacttg atatagagca
     5401 actatcgttt gtattgagta aaaattattt agtttccttt caagaaaaag taggagacca
     5461 ttttaatttt attcgtcaaa gaatgagaga caatctaggt ctagtaagaa agaaagagtg
     5521 cgattttcta ctatttcaat tactcgatgc aattttagat aattattttg aaactcttga
     5581 tgcaatcaat caagaagttg gattgatcga gaaacaaact ttaaccaatc caaagcaatc
     5641 tacactttta ttaattgaaa aaaataaaaa gaatgttagt aaaataaaaa aatcactcgc
     5701 accttttaaa gaagcactta caaatattct acaagataga agtcatttta tcaatgaagg
     5761 aaatcagaaa tactttcgtg atttgacaaa tagctgtagt aatgcaattg aagaggcgaa
     5821 tgcaacctat agcgctcttg agagtctaac aaacatttac ttctcttctt taagccaaaa
     5881 aatgaacgag acgatgaagg ttttaactac tgtcgcaacg atctttattc cactgacttt
     5941 tatagttgga gtttatggaa tgaactttga aaacatgcct gaactcaaat ggcagtatgg
     6001 atattttggc gtatggggaa ttatgataac gactctcttt ggaatgattt tttatttcaa
     6061 aaagaaaaaa tggctttgaa ctattttttt tataaaatca aagagattac ttagaaaacg
     6121 ataggtactt ctttctggtt aaaagaaagt atatgagata actagtgtaa gattctctag
     6181 tttttgtaaa cgataggtgt caataaaaat aggttgaata ataaaggaat gaatatagaa
     6241 tatagggatg aatactgaga agtatatttt gctagttgaa gatgagccta ttaacagtca
     6301 tcttgcaaaa attcagttgg aatcaaaaaa ttacaaagta aaagaagttg ctacaggaga
     6361 agcagcaatc gaactagttt tgaatttaaa ggcttccttt gatctgattt taatggatat
     6421 agatttaggt cccgggatgg atggaacaca aactgccgaa aagatactat ctaaaatcaa
     6481 cattcccatt ctattttact cctctcacac cgaaccagaa attatagaaa aaacggaaaa
     6541 aattacctct tatgggatat gtggtaaaaa attccggaat cacaatctta gacgcttcta
     6601 tcaaaatggc tcttaaacta tttaatgcca at
//
LOCUS       DAOQOS010000205         6600 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_6667_length_6600_cov_5.794500, whole genome shotgun sequence.
ACCESSION   DAOQOS010000205 DAOQOS010000000
VERSION     DAOQOS010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 6600)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6600)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6600
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6667_length_6600_cov_5.794500"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(228..467)
                     /gene="hfq"
                     /locus_tag="PLF66_24935"
     CDS             complement(228..467)
                     /gene="hfq"
                     /locus_tag="PLF66_24935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001274793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006378 - mRNA polyadenylation [Evidence
                     IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HMZ67357.1"
                     /translation="MSTKNNIQDYILNTARKDKIDLTIYLLNGVPLKGKVYSFDNFTI
                     IIENEGKQQLVYKHAVSTIIPLKPIKFAPEEIKES"
     gene            complement(483..1355)
                     /gene="miaA"
                     /locus_tag="PLF66_24940"
     CDS             complement(483..1355)
                     /gene="miaA"
                     /locus_tag="PLF66_24940"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HMZ67358.1"
                     /translation="MLASPTGGGKTELCRQIDPKRFEVVSFDSRQVYKILSVGTAKPD
                     KDTLSYIPHHLVDFLEPNERITAGFFVKKAEEAIEDIYRRSKIPVITCGTGFYLKAFL
                     YGMYSVPEVDSEIKDKLQKMTREERWELLLAKDKIATESIGFADDYRVMRALEVILSG
                     NIKWSEIKQSKLEGFLDLKKPDVTGMLIYRERQELYDRINQRCKQMIESGILEETKEV
                     VVKYGENAPALNSLGYNFALDYINGKMSMESFLEEFSRSHRNYAKKQITWFKKERVLD
                     TMNWATALEKIKNI"
     gene            complement(1370..1798)
                     /locus_tag="PLF66_24945"
     CDS             complement(1370..1798)
                     /locus_tag="PLF66_24945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004785352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FYDLN acid domain-containing protein"
                     /protein_id="HMZ67359.1"
                     /translation="MTKKLQKKVVKKAEPKKAEPSKKADTGKKAEATTKAAKVPIKKP
                     AATASADGNGLGKKHTCYSCATKFYDFGREEKICPKCGADQSTRPAVKVRVPKSSPKL
                     SEFDVVDEDVVAELGTEDDILLEPDAEMDDPEPIIDEEEA"
     gene            1921..2808
                     /locus_tag="PLF66_24950"
     CDS             1921..2808
                     /locus_tag="PLF66_24950"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HMZ67360.1"
                     /translation="MPTFYETVSSIGHKISVKRTINQKKDTMVLLHGFNDNKETFGFL
                     EEFLSKRFNLISFDFRGHGDSDWKKDSLYNYSENLIDVNNVLEHYCKEPIVLLGHSMG
                     AGMAARFTGLFPEKVKLLICLEGFSGLQPQEVERNRLREWLTNISRRNERHTNSAGER
                     KKNMTLEEAKSKLSFIYGKLAKEKIDILINGLVRETEDGSFKWKNDPNLKTSSPIPFP
                     PELSRKLWSEIICPVLIFFGRQTHIRPKNLEEILSHFKNVELHEIEHAGHNMHHDRPE
                     VLIEIMDKFFIQNGFESIP"
     gene            complement(2867..3376)
                     /locus_tag="PLF66_24955"
     CDS             complement(2867..3376)
                     /locus_tag="PLF66_24955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67361.1"
                     /translation="MNLFRKKLFILGFVLFATVIFGQDRKKVDFIAYRGGWFEIQYPK
                     DFSVKPSIESNLPDKYDSAFFISPDGKVKFYIYSPQWNGEATDIIRDSEIEIEKDARK
                     NQKNGITTKWYTYEKKDGTGLRSYQEILDETGYTKYVIGIEYTDREIYKKYRDDFLKF
                     KKTLKQFAD"
     gene            3637..4746
                     /locus_tag="PLF66_24960"
     CDS             3637..4746
                     /locus_tag="PLF66_24960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67362.1"
                     /translation="MKKLLIFFILIPFHSLFSETLVLKDGTIIRMTLKAQDVTSLTYT
                     LKGKEFKVPKDKIRRVVYAKSPELEEKIAKEEVQKMRQEKAAKTPAKSKEEIEEEARL
                     LEEEINKALEEQRRQELLNLSFEERIQRLEKEVGDMKSVTGESASVKLNRIEQDISDL
                     KKRTRRIERFLEIDPDIEEYYNKPRSMWSIVWRSALIPGWGLSYGRDSGFGSMYTTLF
                     FITALGGAGYKSALNSMEKTLNNTFINDFIVQPYALNVLTSSNPSLSSSGTLSTYQTY
                     NITNKFVKYTQSRDSFHHQVENSEKVIQAAVGIYALQLIHSAIYGYFWAKKNPSKFNE
                     EKSSGWNLNIVPLARRNPITNSQDWQMEMGYRVNF"
     gene            4733..6055
                     /locus_tag="PLF66_24965"
     CDS             4733..6055
                     /locus_tag="PLF66_24965"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ectonucleotide
                     pyrophosphatase/phosphodiesterase"
                     /protein_id="HMZ67363.1"
                     /translation="MSISSCFLWLLSIILFLQCSLIERDSPNSIFQHRKLIILSIDGF
                     PGYYLDKDSKFKDLTPNLNRLLVRSNFSNKVDSVYPTLTYPAHTSMITGVDPIQHGIL
                     YNSPVDPFRKYLAGWFWYDEDIQVKTILDFAKDRGLKTASLYWPVTVGAEIDYNIPQY
                     WRSKTEEDEKLLKALSSKNLYKELRKETGLSVLETTGDKEKIEAAIAIWEMKKPDILL
                     IYTTDLDSIHHEKGVFSQQAEEKLRKIDSLLGRLIRKIDLYNNPNLGFIVVSDHGFKE
                     VKSVCAPNKILQSMGVLDSKESKWRYFFKTLGGMAILFENKEKDSLPVSLDLEELKNK
                     LSLECPFALLDTEEELKNIKTKVSKQAKAVLHSKANMAFSESLTVETYREMNYYNHGF
                     LPTDPELKTIGIVYPKSNPIQIQDLKDTFEAGCNWMNLKCQRGANRNR"
     gene            complement(6126..6344)
                     /locus_tag="PLF66_24970"
     CDS             complement(6126..6344)
                     /locus_tag="PLF66_24970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008589921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HMZ67364.1"
                     /translation="MGRIAYVNKDDCTSCNQCADNLPKYFQMDDNDNSETHINGGSIN
                     NAMIPDEDIKIVQKEMDECPGECIKWKN"
BASE COUNT         2217 a         1161 c         1056 g         2166 t
ORIGIN      
        1 aggagtagag actctagacc ttggccagaa tcgctcccct tttcctcccg ccataatcaa
       61 aactacaggt ttttcttttc ctgtcatcga tcttctcctt tcaaatgcaa aataatgtaa
      121 ctagataaaa gcatgatata tttgtctatt ttcaatttga gtcttttaat tctattgaat
      181 tggaatcaga attagagttt atatctaata actgatacaa ttaagaatta gctttctttt
      241 atttcttccg gagcaaattt gattggtttg agtggaataa tggtagaaac ggcatgttta
      301 taaactaact gctgtttacc ctcattttca atgataattg taaaattatc aaagctatat
      361 acctttccct ttaaaggcac accatttaaa agatatatgg ttaaatcaat tttatctttt
      421 ctggctgtat tcagaatgta atcctgtata ttattttttg tagacatccc tttttaccat
      481 ctctatatat ttttaatttt ctcgagagca gttgcccagt tcattgtgtc taaaactctt
      541 tctttcttga accaagttat ctgtttttta gcatagtttc tatgcgaacg agaaaattct
      601 tctaaaaagg attccatgct cattttacca ttaatataat caagtgcaaa attatatcca
      661 agagaattta aagcaggagc attttcccca tatttcacca caacttcttt tgtttcctcc
      721 aaaattcctg attctatcat ctgtttacat ctttgattga tacggtcata taactcctgc
      781 ctctctctat agattagcat tccagtaaca tctggttttt ttaggtctag aaatccctct
      841 agcttggatt gtttaatttc agaccatttt atattcccag acaaaatgac ctcaagggct
      901 ctcataactc tatagtcatc cgcaaatcct atggactctg ttgcgatttt gtcttttgcc
      961 agaagaagtt cccatctctc ttcccttgtc attttttgca gcttatcttt tatttcagaa
     1021 tccacttccg gaacggaata catcccatat aaaaatgctt ttagataaaa accagttcca
     1081 caggtaatca caggaatttt agatcttcta taaatatctt cgatcgcctc ctctgccttt
     1141 tttacaaaga aacctgcagt tatcctctca tttggttcca agaagtctac aagatgatgg
     1201 ggtatatacg agagggtatc tttgtctggt ttagcagttc ctacagaaag tattttatat
     1261 acctgtctag agtcaaaaga aacaacctca aatcgctttg ggtcaatctg acggcatagc
     1321 tctgttttgc ctccccccgt tggagaggcg agaataatta gttttctaat taggcttctt
     1381 cctcatctat aattggctct ggatcgtcca tttccgcatc tggctctaat agtatatcat
     1441 cttcagtgcc taattctgct actacatctt catcaacaac atcaaactca cttaatttgg
     1501 gggatgattt cggtactcga actttcacag ctggtcgtgt gctttgatct gcgccacatt
     1561 ttggacaaat cttttcttct ctgccaaaat cataaaactt tgtagcacaa ctataacagg
     1621 tatgtttctt tcctaaaccg ttcccatctg cggaagcggt ggcagctggc tttttaatcg
     1681 gtactttggc tgctttggta gtagcttctg ctttcttacc tgtatcagct tttttagaag
     1741 gttctgcctt ttttggttca gccttcttaa caactttttt ctgcaacttc ttggtcataa
     1801 aaaagaaatt aaaaaatcta ccattcaaat aatgacaagc taatttttga aattttatta
     1861 tttttctatt tttttttcaa aataggtaga ctagattcaa aaatagaatt tcttacacgt
     1921 atgccaactt tttatgagac agtttcttcc ataggtcata aaatcagtgt gaaaagaacc
     1981 ataaaccaaa agaaagatac catggtttta ctccatggat tcaatgataa taaagaaaca
     2041 tttggttttt tggaggagtt tttaagtaaa cgctttaatc taattagttt cgattttagg
     2101 ggacatggag actctgattg gaaaaaggac tctctctata attatagcga aaacctaatc
     2161 gatgtaaata acgttttaga acactattgc aaagagccta tcgtcctttt aggacattcg
     2221 atgggagctg gaatggccgc aaggtttaca ggattatttc ctgaaaaagt taaacttttg
     2281 atttgcctag agggttttag tggtttacaa ccacaagaag tggagagaaa tcgactccgt
     2341 gaatggctaa caaatattag ccgtagaaat gaaaggcaca caaactctgc cggggaaaga
     2401 aaaaaaaata tgactctaga agaggctaaa tccaaacttt cctttatata tggaaaatta
     2461 gcaaaagaaa aaatagatat tctgataaat ggtctagtcc gagaaacgga ggatggctct
     2521 ttcaaatgga agaatgatcc taacttaaaa acttcttccc ctattccctt tccaccagaa
     2581 ctttctcgaa aactatggag tgaaattatt tgcccagtct taattttctt cggacggcaa
     2641 acacacattc gacccaagaa tttagaagaa atactttctc attttaaaaa tgttgaatta
     2701 catgaaatag aacatgcagg tcataatatg catcacgata gaccagaagt attaatagag
     2761 attatggaca agttttttat acagaatggt tttgaatcga ttccctaatt ctattgatac
     2821 tgatatctca ttcatttgaa tactatctcg aaatcaatga ctgatctcaa tctgcaaatt
     2881 gtttcaatgt ttttttaaac tttaaaaaat catctctata tttcttatag atttctctat
     2941 cggtatattc aatacctatg acatactttg tataacctgt ttcatctaat atttcttgat
     3001 aagatcttaa accagttcca tccttttttt cataggtata ccattttgta gtaataccat
     3061 ttttctgatt ttttcttgca tccttttcta tttcgatttc tgagtcgcgg ataatatctg
     3121 ttgcctcacc attccattga ggagagtaaa tataaaattt tacttttcca tcaggagaaa
     3181 taaaaaaagc actatcatat ttatctggaa gattagattc aatagaaggc ttaacggaaa
     3241 aatcttttgg atattgaatt tcaaaccagc ctcccctgta tgctataaaa tctactttct
     3301 ttcggtcttg gccgaagata actgtggcaa atagaacaaa acccaagata aataattttt
     3361 ttctaaataa attcataagc tacaagtttt taaacggatt caaattgggc aaccgtaaag
     3421 ataaaattag aagcagaatt cagaaattag aatctctttg aattcagcca atttataaag
     3481 cagacattca tttctattaa attcaaaatt tgtattcttt tcctttttaa attacggaaa
     3541 caaagattta ggatagtaaa tcaatcctta ccctaaaatt atcttgcaac gatttagttg
     3601 aaataaaaga gttatttaaa ctatggaaaa ccaactatga aaaaattatt aatattcttt
     3661 attctaatcc cattccattc tttattctca gagactttag ttttaaaaga tggaactata
     3721 atacgaatga ctcttaaagc acaggatgtg actagcctca catacactct aaaaggaaaa
     3781 gagtttaaag ttccaaaaga taaaattcgt cgtgttgtgt atgcaaaatc tccagaacta
     3841 gaagagaaaa ttgcgaaaga ggaagtgcaa aaaatgagac aggagaaagc ggcaaaaact
     3901 cctgctaaat caaaagaaga aatcgaagaa gaagcaagac ttcttgaaga agaaatcaac
     3961 aaagcactcg aagaacaaag aagacaagaa ctactcaatc taagctttga agaaagaata
     4021 caacgcctag aaaaagaagt aggagatatg aaatctgtca caggcgaaag tgcgtctgta
     4081 aaattaaatc gaatcgaaca ggatattagt gatcttaaaa aacgaaccag aagaattgaa
     4141 agatttttag aaatcgatcc agatatagag gaatattata ataaaccaag atctatgtgg
     4201 tctatcgttt ggcgttctgc tctaatacca ggctggggtt taagttatgg aagagatagt
     4261 ggttttggat ctatgtatac aaccttattt tttataacag ctcttggtgg agcaggttat
     4321 aaatcagcat tgaatagtat ggaaaaaacg ttaaataata cattcatcaa tgactttatt
     4381 gttcagcctt atgcgcttaa cgtattgact agctcaaatc cctcgctatc ctcatctgga
     4441 actctaagca cataccaaac ctacaatatc acaaataaat ttgtgaaata cactcagtct
     4501 agagactcct ttcatcacca agtagaaaac agtgaaaaag tgatacaagc agcagttgga
     4561 atctatgctc tacaattaat ccactcagct atctatggat acttttgggc aaagaaaaat
     4621 ccaagtaagt tcaatgaaga aaaatcctct ggttggaatc taaacatagt tccacttgca
     4681 aggagaaatc cgattacaaa ttcgcaagac tggcaaatgg aaatgggtta tcgtgtcaat
     4741 ttctagttgc tttctgtggt tactgagtat tatcctcttt ctacaatgca gtctcataga
     4801 aagagattct ccaaattcta tttttcaaca tagaaaacta atcattctat ctattgatgg
     4861 ctttccagga tattatctag acaaagattc taaattcaaa gatttaactc ctaatttaaa
     4921 tagactttta gttagatcta acttttctaa taaagtagat tccgtttatc caactctcac
     4981 ctatcctgct catacttcta tgattaccgg agtcgatcca attcaacatg gaattcttta
     5041 taattctccg gttgatccat ttcgaaaata tttggctggt tggttttggt atgatgaaga
     5101 tatacaggtg aaaactattt tagattttgc aaaggacaga ggtctaaaaa cagcttctct
     5161 ctactggcct gtaactgtcg gtgcagagat agattataac attccacagt attggagatc
     5221 aaaaacagaa gaagatgaaa aactattaaa ggctctttct agcaaaaatc tatacaaaga
     5281 attaagaaaa gaaacgggac ttagcgtcct agaaacaacg ggcgataaag aaaaaataga
     5341 agcagctatt gccatatggg aaatgaaaaa accagatata cttttgattt atacaactga
     5401 tttagattct attcatcacg aaaaaggagt cttcagccaa caggcggaag aaaagctaag
     5461 aaaaattgac tctctcctag gaaggctcat tcgtaaaatc gatttatata acaatccaaa
     5521 cctagggttt atcgttgtct ctgatcatgg atttaaagaa gtaaaaagtg tatgtgctcc
     5581 aaataaaatt ttgcaatcaa tgggagtttt agatagtaaa gaatctaaat ggagatactt
     5641 ttttaagacg ttaggtggta tggcaattct ttttgaaaac aaagaaaaag attctttgcc
     5701 agtcagtttg gatttagaag agttaaagaa taaactcagt ttggaatgtc cctttgccct
     5761 tctcgacaca gaagaagaat taaaaaatat taaaacaaag gtttctaaac aagcaaaagc
     5821 tgttcttcat tccaaagcga atatggcgtt ttcagagtcc ctcacagtag aaacgtatcg
     5881 cgagatgaat tattataatc atggatttct tccgacggat ccagaattaa aaacgatagg
     5941 cattgtgtat ccaaaatcaa atccaattca gatacaagat ttgaaagata catttgaagc
     6001 aggttgcaat tggatgaatt taaaatgcca aagaggtgcg aatcgaaata gatagaaaac
     6061 ttgctataga gatttttctc taatagcaag tgatagttaa ctcaataatc taaaatggag
     6121 tgaaattaat tcttccactt aatacattca cccggacatt cgtccatttc tttttgaacg
     6181 atcttaatat cttcatccgg aatcattgca ttgttaatag acccaccatt gatatgagtt
     6241 tccgaattat cattgtcatc catttgaaaa tactttggaa ggttgtccgc acattggttg
     6301 caagaggtgc aatcatcctt gtttacataa gcaattcttc ccataaattt ctctccttac
     6361 gtatggaata cttttatttg catagtattc aaggaatatt cctaggtcaa gtgaatatag
     6421 tttgtagaca tgcactagaa attaaggatt ttttagatta aattttctag gtgctctctc
     6481 taatgataag tttttaaacc catcttagta tctccaatca aattcctttt tttataaatt
     6541 ttattctatc agtctgttgc gaaatgttaa agaattaaaa gtcagccttt aaaaataaat
//
LOCUS       DAOQOS010000206         6585 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_6689_length_6585_cov_5.309495, whole genome shotgun sequence.
ACCESSION   DAOQOS010000206 DAOQOS010000000
VERSION     DAOQOS010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 6585)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6585)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6585
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6689_length_6585_cov_5.309495"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(5..256)
                     /locus_tag="PLF66_24975"
     CDS             complement(5..256)
                     /locus_tag="PLF66_24975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014804454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HMZ67365.1"
                     /translation="MKKKMIYKDAPLEISKAIKTAKVIKDFLPKPDDLVLKDKTVRIM
                     INLNKNSVDFFKKESQRLGVPYQKISLLRNKKKGLQNRF"
     gene            1022..1294
                     /locus_tag="PLF66_24980"
     CDS             1022..1294
                     /locus_tag="PLF66_24980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67366.1"
                     /translation="MKLSLKFFFIFLFFLQISCRTLFEIGNGNFFPYSGTAKNWKCIT
                     KKTENENSKLEEPLKLICFIDFPMSFAFDTIFLFITIPISLKQDKE"
     gene            complement(1714..2319)
                     /locus_tag="PLF66_24985"
     CDS             complement(1714..2319)
                     /locus_tag="PLF66_24985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67367.1"
                     /translation="MSINPNWKKCFFLFLFLNCADYEKRNGQFIQEDKYVKNAIQIQL
                     DGINKDSIYSPEIHLGLYTDTYNFGEIPHYLFQKRGKENTFLMDLPEGEFQGSLTIKD
                     NRYYPPFYSEWSSIKIYFGNGPSDTRDRIHSDGEECKNIDQYGQIFICPKLKIKKNTQ
                     VQFHFRIDSTSSVGYNETGLVYLMVFAVSFRTFSLLDLEFF"
     gene            complement(2326..2697)
                     /locus_tag="PLF66_24990"
     CDS             complement(2326..2697)
                     /locus_tag="PLF66_24990"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HMZ67368.1"
                     /translation="MEERDRSKSQGRREDRRHLIYYLKVENKETNELIGRIVDITSKG
                     ILLISRDKISPNTEYSVRIELGDELFDYLHGHLEMHIRCRWSKEDINPDYFVNGFEFI
                     NQSTEQEFIIRNLIEVIGFVE"
     gene            complement(2821..3015)
                     /locus_tag="PLF66_24995"
     CDS             complement(2821..3015)
                     /locus_tag="PLF66_24995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67369.1"
                     /translation="MQAILLNFVVSFAVWGSVHFNALSLASITAKASSEFQNTDLSNV
                     LPEEITNELKKIEDEKKESI"
     gene            3021..3206
                     /locus_tag="PLF66_25000"
     CDS             3021..3206
                     /locus_tag="PLF66_25000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67370.1"
                     /translation="MPKECTIDGVGTRTPTGKITIYANTPITRIKEMMGNTYYWDQVE
                     GRNTPNPNDDWYQRKTR"
     gene            3333..4301
                     /locus_tag="PLF66_25005"
     CDS             3333..4301
                     /locus_tag="PLF66_25005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002996550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 43"
                     /protein_id="HMZ67371.1"
                     /translation="MNQFTLEQIKVIQWKEFDKNPILNPPIFSPILADPSFLFPEESP
                     NSLWNLFAHSIYGIHHYTSKDGIKWIYNKHLFFNAMRPYIFKEDDRYYILYEKYHYFQ
                     ILYSWMKAFKWNSHLEIRESKDLIHWSKPHTILSPTLDWHKNNTLGNSVSNPCLLKVG
                     EEYRLYFSSSLSFIPDCGFSEPRFIGLARSKNILGPYLPDNAPILSPKDSDPWMNLAA
                     GSIKVIQIKDGFLGFQNGIYLENNQSGSAILLMHSKDGINWNRVHSNPILKPYSGWMR
                     SHVYALDVKYNSSFQKFYLYFNARNDWHWTKGKENIGLLYGEGKEI"
     gene            4356..4973
                     /locus_tag="PLF66_25010"
     CDS             4356..4973
                     /locus_tag="PLF66_25010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67372.1"
                     /translation="MFKSIVTKKFSSVLFLAFALLTNCIEFHTRDYNESRPISSYTNK
                     IELQVIPEKASQGCERDFKEPVQTTFIKLLNNENLIANNSADIVLLEKCEKEKAGEIK
                     WIFLANFTLTLWSWGLIPCYAKHIEKASIAVSYKNREFKTYNYENVRHTIIWLFFFPT
                     LFFDNTKPYERFLSATGQDLYHDLNQTIQKQEEEKLIKELESPKK"
     gene            5111..>6585
                     /locus_tag="PLF66_25015"
     CDS             5111..>6585
                     /locus_tag="PLF66_25015"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HMZ67373.1"
                     /translation="MTKLTKILILALAGTSLILSFIIGGLAYAESKRVTTVEAKNHLK
                     ELLEKNALLLEERIQEVVYFTNYIIEASLRNFDLKKIKNNPINRKMHEEKLVPLFAEG
                     MKASNARSAWIHFDSKVIGGLLHVSFFKQNGKIARSDVFDIYTTGHDKDEWFVKAKNE
                     DTHWTKPYFWEPWGVTLISYSKRVEKEEYIGVAGSDMFMDDLFQEFQKHKIYRTGSIF
                     LMNQNFDLLYTPDPNLKNLKTDSSGKFSFISDKIENDKSNFGIIEFSIDGKDRLIAYR
                     KLTNGWILAAEPFLDEMYETQRKLSIRILFVLILGIIISITISIYLGRAISKPIIELT
                     SELTEKNTELLTKNAEIESQKKELDYYANYDALTGVPNRRAGILFLNQMVENAYRNKN
                     DLTIVFIDVNNLKLVNDKFGHKEGDHLIKTISKYILENIRESDMLARLGGDEFLLILP
                     ESTEDEAIQILTRIEDQIEKDRHLFQYPISFSYGYERYPTY"
BASE COUNT         2338 a         1088 c         1013 g         2146 t
ORIGIN      
        1 tttattaaaa tctattctgt aaaccctttt tcttatttcg caacagactg attttctggt
       61 aaggaactcc aagacgctgg ctttctttct taaaaaaatc aacgctgttt ttatttaaat
      121 tgatcataat tcgaactgtt ttatctttca aaacaagatc atcaggcttt ggaagaaaat
      181 ccttgataac ctttgctgtt ttaattgcct tactaatttc taagggagca tctttataaa
      241 tcattttctt tttcataaaa ttcctttcct tccctccaaa acccagcccc aaaaattcgt
      301 attgaaatct ttctgactgt gaaacgcact gtgagaatac ctcgttcaac cattccaaag
      361 aagaaataac gcttttcctt ttcagttgaa tgttttactc ctcggcaata attcgattct
      421 tatcaaaaaa tgcaacttgc gcctctgaaa aggatacatc atgcttcttt ctgttcttct
      481 gttcttcttt ctcatcccat acaaatttta tacaaatact tatatgtata aaaactcaat
      541 tataaaaacg aacgaattca aatagcaaag agaacgcgaa gaaaagagaa gatttattat
      601 ttactcactt cgtgagtctt cgtatgtttc ttgattaaaa atccttaagt tgacagcatt
      661 aaccgtagca agttctattt attttcatac aaacatatca cccatcttct cttaaactta
      721 ggggtaaaga tccattaaac ttatcctaaa tttatctcca tacctatctt taatggaagt
      781 ttagtcctta ctttagggga tctttagtgt atcgttcata cgagattcac agaaaagcgc
      841 agatactaga agagcaattc tgcatccatg ttgaggagtt ttagctaagg ttttttatcg
      901 aagcggtaag tatttatcta taacgaaaaa gagatcggct atttaagttt gtttaacata
      961 ttttgaagtg gagtgaatag aatggaattg gctatattat acttttagaa agaaaatgat
     1021 tatgaagctt agtttaaaat ttttctttat ttttttattt ttcttacaaa tcagctgtag
     1081 aactcttttt gagataggga atggaaattt ttttccctat tcaggaacag caaagaattg
     1141 gaaatgtatt acaaaaaaaa cagagaatga aaactcaaaa ttagaggagc ccctaaaact
     1201 tatttgtttc attgattttc caatgagttt tgcatttgat acaatttttt tatttattac
     1261 aattccaatc tcactcaaac aggataaaga ataaaagttt tattagttct tctgcggcta
     1321 ctcttaaata gccgcaattt ggtaataaaa aatacactat ctgcatttgt ttaacataat
     1381 acaaggctgg agtgaataga atgcgctcga aattcctagg aaaaaagttt taatttttgt
     1441 ttatcggtaa cattttacaa tcctaattgc tactatggtt tcacaaagac acagagagat
     1501 aaatatacaa actgggtgac cgataacgtt cctaaaaaag attcacaatc tatttttact
     1561 ttaagacttt cacttgtgag atagaatgga agagcagtat gaaaaaagtt ggatgcaaat
     1621 gagccgatag acacaagata tacaatcact ccgtgtattt caattctttg aaagaatgtt
     1681 caatgtttcg tgttcggacc acaacgcctg ttcctaaaaa aattccaaat ccagcagaga
     1741 aaaggttcta aacgatacgg caaataccat taaatagaca agtcctgttt cattgtaacc
     1801 aacagaggat gttgaatcga ttctgaaatg gaattgaact tgtgtattct ttttaatttt
     1861 taatttggga caaatgaaaa tttgtccata ttggtctata ttcttacact cctctccatc
     1921 cgaatgaatt ctatctcttg tatcggaagg tccgtttcca aaataaattt ttatagaact
     1981 ccactcggaa taaaagggcg gataatatcg attatccttt atagttaaag acccttggaa
     2041 ttctccttcc ggcaaatcca ttagaaaagt attttctttg cctctctttt gaaataaata
     2101 atgaggaatt tctccgaaat tatatgtatc ggtataaagt cctaaatgaa tctctggact
     2161 gtagatacta tctttattga tgccgtctaa ttggatttga atggcattct taacgtattt
     2221 atcctcctga ataaattggc cgtttctttt ttcgtaatct gcgcaattta aaaataaaaa
     2281 aagaaaaaaa catttcttcc aattcggatt tatactcatc taggattatt caacaaatcc
     2341 aattacctcg attagatttc gaatgataaa ctcctgttct gtgctttgat tgataaactc
     2401 aaacccattc acaaaataat ctgggttaat atcttctttg ctccaacgac atcgaatgtg
     2461 catttctaaa tgtccatgca aataatcaaa taattcatcg cctaactcga ttcgaacaga
     2521 atattctgta ttgggggaaa ttttatcgcg actaattaag agtatcccct tacttgttat
     2581 atcaacaatc cgaccaatta attcatttgt ttctttattc tctactttta aataataaat
     2641 caggtgacgt ctatcttctc ttctaccttg acttttactt ctatctctct cttccatcgc
     2701 gcctacctca aaatataaac tcagattaaa atacccttac gttaaatata tttcactaca
     2761 agtggaaaga aaaagcaatg attattctag atttccaatg ggtttttaat atgacttttg
     2821 ttagatagat tccttctttt catcctcaat ttttttgagt tcatttgtaa tttcttctgg
     2881 gagaacattc gaaagatctg tgttttgaaa ctcagaagaa gccttagcag tgatactcgc
     2941 aagagaaagt gcattgaaat gcacagatcc ccaaacagca aaggaaacta caaaatttaa
     3001 taagatcgct tgcacaacgc ttgccaaagg aatgcacgat tgatggagta ggaacaagaa
     3061 ctccgactgg taaaatcaca atttatgcaa acacaccaat cactcgtatt aaagagatga
     3121 tgggaaacac ttattactgg gatcaggtag aaggtagaaa tactccaaac ccaaatgatg
     3181 actggtatca aagaaaaaca cgttaattca gtttaaaaaa agaatactac ttattctaga
     3241 caataggtgg tattcagttt ctaacatttc ttccaaaata aaaagaataa aagacttcga
     3301 aataaatcgg tagaaagaaa cattgcattc aaatgaatca atttacctta gaacaaataa
     3361 aagtaattca atggaaggaa ttcgataaaa atccgattct aaatccaccg attttttctc
     3421 ccatcttagc agatccgagt tttctatttc cggaagaatc tccaaatagt ttatggaatt
     3481 tatttgctca ctcaatttat ggaatacatc attatacctc taaagatggg atcaaatgga
     3541 tatacaataa gcatcttttc tttaatgcaa tgagacctta tatttttaag gaagacgata
     3601 gatactatat actctatgaa aaatatcact attttcaaat tctatattcc tggatgaaag
     3661 cattcaaatg gaattcacat ctagaaataa gggaatcaaa ggatttaatt cattggagca
     3721 agcctcacac aattctttct cccactttag attggcacaa aaacaatacg ttaggcaact
     3781 cagtaagtaa tccatgttta ttaaaagttg gagaagaata tagactgtat tttagttctt
     3841 ctcttagttt tattcctgac tgcggatttt cggaacctag atttattgga ttagctcgat
     3901 ctaaaaatat tctaggtcca tatctaccag ataacgctcc tattctttca ccgaaagatt
     3961 cagatccgtg gatgaatctt gctgcaggct caataaaagt gatacaaata aaagatggtt
     4021 ttctaggatt tcaaaatgga atctacctag aaaataatca atcaggatca gcaattttac
     4081 tcatgcattc taaagatgga atcaactgga atcgtgttca ttcgaatcct attctcaagc
     4141 catattctgg ttggatgaga tctcatgtat atgcactcga tgtaaaatac aattcatctt
     4201 ttcaaaaatt ttatctatat tttaatgcaa gaaatgattg gcactggacg aaaggaaaag
     4261 agaatatagg attactttat ggagaaggaa aagaaattta aaatttcttt tcttttgtat
     4321 taaggtctct ctttattaca ttttagggaa aaacaatgtt taaatcgata gtaactaaga
     4381 aattttcttc tgtattattt ttagcttttg ctttactaac caactgtatc gaatttcaca
     4441 caagagatta caatgagtcc cgacctattt cctcttatac gaataaaata gagttacaag
     4501 tcattcctga aaaagcaagc caaggctgcg agagagattt taaggaacca gtgcaaacaa
     4561 catttattaa acttttaaat aatgaaaatc taatagcaaa caattcagcg gatattgtgc
     4621 ttcttgaaaa atgtgaaaaa gaaaaagcag gagaaattaa gtggattttt ctagctaact
     4681 ttacacttac tctctggagt tgggggctta ttccttgtta tgcgaaacat atagaaaaag
     4741 caagtattgc tgtttcgtat aaaaacagag agtttaaaac ttacaattat gaaaatgtca
     4801 ggcatacaat tatttggtta ttttttttcc ctactctttt ttttgataat acgaaaccgt
     4861 atgaaagatt tctttctgct acaggtcagg acctctacca tgacttaaat caaaccattc
     4921 aaaaacaaga agaagaaaaa ttaataaaag aattggaatc accaaaaaaa taaaaacagt
     4981 tccatagaaa tagaaaacta gtggcaattc actgttaccc gattgttttt acgatccatt
     5041 tcatttatgg aaagattgac agcgttttta agaaattaca aattggtata aatcattttt
     5101 attagaacga atgacaaagt taacaaaaat attgatatta gctcttgccg gaacttcact
     5161 tattttatct tttatcattg gcggacttgc ttatgctgaa tccaaaagag ttacaacggt
     5221 agaagcaaaa aatcatctca aagaattatt agagaaaaat gctctattac ttgaagagag
     5281 aattcaagaa gtagtctact tcaccaatta tatcattgag gcttccctac gcaactttga
     5341 tctaaaaaaa ataaagaaca atccaataaa tcgtaaaatg catgaagaaa aattagttcc
     5401 tctttttgca gagggaatga aagcgtcaaa tgcaagatct gcctggattc actttgactc
     5461 aaaagtgatt gggggtttac tacatgtttc tttttttaaa caaaatggaa agatagcaag
     5521 aagtgatgta ttcgatatct atacaactgg tcatgacaaa gacgaatggt ttgtaaaagc
     5581 aaaaaacgag gatacgcatt ggacgaaacc atatttttgg gagccttggg gagtaacctt
     5641 aattagttat agcaaaagag tagaaaaaga agaatatatt ggagtagcag gatctgatat
     5701 gtttatggat gatttgtttc aagaattcca aaagcataag atttatagaa ctggttccat
     5761 ttttctgatg aaccaaaatt ttgatctact ctatacacct gaccccaatt taaaaaattt
     5821 aaaaacagac tcatcaggaa agttttcttt tatctctgac aaaattgaaa acgataaaag
     5881 taattttggt atcattgagt tttcaataga tggaaaagat agactcatcg cctatcgcaa
     5941 acttacaaac ggttggattc ttgccgcaga acctttctta gatgaaatgt atgaaactca
     6001 gagaaaactg agtattcgta tattatttgt tttgatttta gggatcatca tctcaatcac
     6061 tatttctatc tatttaggta gagctatttc aaaaccaatt atagaactaa cgtctgaact
     6121 aacagagaag aatacagagc ttttaactaa aaatgcagag atagaatcgc aaaaaaaaga
     6181 actagattat tatgcaaatt atgatgcgct cactggagtt cccaatcgaa gagctggtat
     6241 tttatttcta aatcaaatgg tagaaaatgc ctacagaaat aaaaatgatc ttacaatcgt
     6301 atttatagat gtaaataatc taaaacttgt aaatgataaa tttggtcata aagaaggaga
     6361 ccatctgatc aaaactatat ctaaatatat tttagaaaac attcgcgaat cggatatgct
     6421 agcaagacta ggcggagatg aattccttct catacttcct gaaagcacag aggatgaagc
     6481 aattcaaatt ttaacaagaa ttgaagacca aattgaaaaa gatagacact tatttcaata
     6541 tcccatcagt tttagttatg gatatgaaag atatcccact tataa
//
LOCUS       DAOQOS010000207         6532 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_6759_length_6532_cov_6.103906, whole genome shotgun sequence.
ACCESSION   DAOQOS010000207 DAOQOS010000000
VERSION     DAOQOS010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 6532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6532
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6759_length_6532_cov_6.103906"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(106..819)
                     /locus_tag="PLF66_25020"
     CDS             complement(106..819)
                     /locus_tag="PLF66_25020"
                     /inference="COORDINATES: protein motif:HMM:NF020916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate polymerase domain-containing
                     protein"
                     /protein_id="HMZ67374.1"
                     /translation="MSEKKKYRHELKYLINYQEYFLIRSRVRSVLPSDSNVDGDGSYT
                     VRSLYFDDYNNSSYYDKYAGILKRAKHRIRIYNYSDQRINYEKKNKYGQYNHKETALI
                     TKEQFYAILSGDFEFLLKSPNSLLSKLYYECRINLLRPRIIVDYEREPYVLAAGDVRL
                     TFDKNIRAGIDGFDIFDKDIPTIETMDPGTLVMEVKYTEFLPNIVRDILASSSALYVE
                     SSKYILCCDKMLHTRGFNT"
     gene            complement(937..2745)
                     /locus_tag="PLF66_25025"
     CDS             complement(937..2745)
                     /locus_tag="PLF66_25025"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HMZ67375.1"
                     /translation="MKKFSNYTLSIIVTFLLFGSLNSLSESLTSVEINKSNNTPISPA
                     TPSQTLVNITSVEDVNNQPIKDNLALYENDDPTSVVCMYVTVRKGNSLENTEYTWEEV
                     NNFDKWTNKGSRAANDVVGQAEVIFQIGDETGPLPGELGYNISVPNGTIQIRGASTSA
                     NGNQKSYKIELYDDAGLWRGQSTIALNKHVFDPTRVRNKLSFDLLKGIPGIVTLRTQF
                     VHLYVKDETTSFAGKGFVDYGLFTQVEAVNKAYLRNHQLDPNGQLYKATSFEFYRYED
                     QIRLESDPLFDESEFSHRLEIKGNRDHSKLIQMLNDINNYDIPIAQTFEKYFNEDNYF
                     TWLAYNILVGNVDTQNQNFYLYSPQNSEKWYFIPWDFDGDLERQKKEELDSDPYLSWE
                     VGLHNYWGGVLHNRLLRLEKYRTMLDNKINELSIAFPADRLRTMLNSYRAVTEPFVFS
                     APDVKHLSRNKYQYNLSFELIPTEIENNKKLYFETQKYPTPFFLGTPEIIDGELRFTW
                     DQSFSFTPQVITYRFLISKDLLFNNIAFQSDLVESTEISTPVFEPGVYYWRVIAINEE
                     GFNRFPFDAYFGQGFSRYDGMKFFMINQDGTLGDAQ"
     gene            complement(2742..4508)
                     /locus_tag="PLF66_25030"
     CDS             complement(2742..4508)
                     /locus_tag="PLF66_25030"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HMZ67376.1"
                     /translation="MKAFFRAVLMIIAVFVVIIISLLSLSPNSSIDKNLTVSKLVATQ
                     QELLTENNISVDQSIFQDNADIYINDDSSSVVTIFITVMENDAETWAAMNEYKTLAAT
                     NNTNMDKKNVEAIVQFGDDKGPLPTEFGFNDITPNALVELRGANSAFDSQKSFKLTLD
                     NSSGAWRGQSIINLNKHLSDETRFRNKLSFDLMKGIPHLVTLRTQFIHLYVKIESPEG
                     QKGFIDYGLFTQVEQPNRRFLDSRLLDSNGQLYKANNFLFNRYPDQIHLVTDPLYSED
                     AFSQVLEIKGDEDHSKLIRMLDELNNSDIPIENTFEKYFDSENYFTWMAFNILTGNVE
                     AVSQNYFLYSPKNGDKWYFIPWDYDKAFPLQSNAKLATKTYLPWQRGVSNYWYSTLHR
                     RVLENDQYQNKLDKKIDEVLGYLNSDVINAFVQRYQPVTDKYSFAMPDAFHMPVDINI
                     SHQIANLLASDIDNNYKLYVESLETPMPFVMEAPIINGDNVDFVWGSSYDLGSRDLTY
                     QFVLGKDWAFKEVLYQKSTKDTNIEVPLNLLAPGKYFWRVTAINSLGKVQYSYNYYTD
                     ANSEIHPGIKVFYFTTNNQVFE"
     gene            complement(4505..5956)
                     /gene="pelG"
                     /locus_tag="PLF66_25035"
     CDS             complement(4505..5956)
                     /gene="pelG"
                     /locus_tag="PLF66_25035"
                     /inference="COORDINATES: protein motif:HMM:NF028243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide Pel transporter PelG"
                     /protein_id="HMZ67377.1"
                     /translation="MAGIGFELKKLFAKKGILLQLRANAYTSMVVAGPMLLGAIFLLG
                     TRYVAEFGNATIHQQDLIVVIITYSLLFSLLLTSTLLYVLTRFVADMMYIKEYGRILP
                     SMYGAISLFLVVGSVLWGIFLAFSGIQFSYAVYSFILFCEGIVAWVQIGYISALKEYK
                     GILIGFSVSIFSGLAFGYIFILLNFEVVSSLLLGACIAYGILVVAFTIEIHQFFPMGS
                     GSSLRFLEWIDKYPHLPFIGFFSTFGLFIHLMLMWFSPLGVQVEGLFYHAPRHDIPAL
                     LAFTTSLVSAVNFVATVEVNFYPKYKLYFNLLNGEGSLSNIETAYNDMIDVLKREIFY
                     LAIQQVFFTLLAITLLGEFLIHLNLGFTLGMQEMFRVLCVGYGIYAIGNVLILFLLYF
                     SDNQDALWSSALFCISNVLITWYTIKTSQTLYGFGFVLAAVVLYIVSLRSLYLYTDKL
                     DYHTFTKQPVFFVQKRSFFTRIANQLDLIEVKK"
     gene            complement(5932..>6532)
                     /gene="pelF"
                     /locus_tag="PLF66_25040"
     CDS             complement(5932..>6532)
                     /gene="pelF"
                     /locus_tag="PLF66_25040"
                     /inference="COORDINATES: protein motif:HMM:NF038011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GT4 family glycosyltransferase PelF"
                     /protein_id="HMZ67378.1"
                     /translation="EKPKDDWIDIAIIARFAPIKDIKTLIYTFSRLKQEIDNARLHII
                     GGIDDEEYHQECLELIKYLDLRDIIIPGTVRTTSYLEKIDFTILTSISEGQPFAVLES
                     LAARRPVVSTDVGCCRELLEGDEGDKFGQAGICVPPMHQYKLFQALMEMCHSEDNRKQ
                     MGMAGQQRTIKFFDINDMKKNYLSVYERAISKWQESASN"
BASE COUNT         2249 a         1266 c          990 g         2027 t
ORIGIN      
        1 aaacgaccgg cgccaacaat gctgacgccg cggcaaaatc catctgtaaa aaattaagta
       61 tattttccat tattatgcct ctttacaaac gaaatagtat taatatcagg tgttaaaacc
      121 ccgagtgtgc aacattttat cacaacaaag aatatatttt gaagactcta catacaacgc
      181 agaagatgaa gctaaaatat cccttactat attaggcaaa aattcagtat acttgacttc
      241 catcactaaa gtaccaggat ccatagtctc aatagtcggg atatctttat caaaaatatc
      301 aaaaccatca atacctgcac gaatattttt atcgaaagta agccgaacat caccggctgc
      361 caatacataa ggctcacgct cataatccac aataattcta gggcgcaata aatttatcct
      421 acattcataa tagagtttgc ttaataagct attcggactt ttcaataaaa actcaaaatc
      481 accacttaaa atcgcataaa attgctcttt ggtaattaat gcagtctctt tatggttata
      541 ttgcccatat ttgttttttt tctcataatt tattctttga tcagaatagt tgtatattcg
      601 aatacgatgt ttggcgcgct tcaaaatacc tgcatattta tcataataag agctattgtt
      661 gtagtcatca aaatacaaac ttcggactgt gtaagatcca tcgccatcta catttgagtc
      721 agatggaagt acactcctta ctcgagaacg tatcaaaaaa tattcctgat aatttattaa
      781 gtacttaagt tcatgacgat atttcttctt ttctgacatt tttcaccaaa tagtggtttc
      841 tatataaagt catcacatac cggtaatatt tatgttttta ttgtaaaaca caattattac
      901 tgataacccc taaagagtaa tttatacaat aatcaattat tgagcatctc ctaaagtccc
      961 atcttgattt atcataaaaa acttcatgcc gtcataccta ctaaaacctt gtccaaagta
     1021 cgcatcaaat gggaaacgat taaaaccctc ttcattaatt gctataaccc tccaataata
     1081 aactcccggt tcaaacacag gtgtactaat ctctgtgctt tcgactaagt cagattgaaa
     1141 ggcaatatta ttaaaaagta aatctttact tattaagaat ctataggtaa tgacttgcgg
     1201 tgtaaaacta aatgattgat cccaagtaaa tctaagttcg ccatcaatta tctcaggagt
     1261 acctagaaaa aaaggtgtag gatatttttg ggtttcaaaa tatagttttt tgttgttttc
     1321 aatctcagta ggaataagct caaaagagag gttatattga tacttgtttc tagataagtg
     1381 tttaacatca ggggcactga aaacaaaagg ctccgttacg gctcgataac tattcaacat
     1441 tgttcttagt ctatcagcgg ggaatgctat gctcaattca ttaattttat tatccagcat
     1501 ggttcgatat ttttccaaac gaagcaagcg gttgtgcaat accccgcccc aataattatg
     1561 gagtccaact tcccaactta agtatgggtc tgagtcaagt tcttcttttt tttgtcgttc
     1621 caaatcgcca tcaaaatccc aagggataaa ataccatttt tcgctgtttt gaggactata
     1681 caaataaaaa ttttgatttt gtgtatcaac attcccaact aatatattgt acgcaagcca
     1741 agtgaaataa ttatcttcat tgaagtattt ttcaaaggtt tgagctattg gaatatcata
     1801 attatttata tcattcaaca tttggatcaa tttggagtga tcacgatttc ctttgatttc
     1861 caaacgatgt gaaaactcgc tttcatcaaa caaagggtct gactcaagac gaatttgatc
     1921 ttcgtatcta taaaattcga atgaggtcgc tttatacaat tgcccattag gatccaattg
     1981 gtgattcctt agataagctt tgtttaccgc ttctacctgt gtaaacaagc cataatctac
     2041 aaatcctttc cctgcaaacg aagtcgtttc atctttaacg tataaatgaa caaactgcgt
     2101 ccgcaaggta acaataccag gaattccttt tagaagatca aaggacaact tatttctaac
     2161 gcgtgtagga tcaaagacat gcttattaag tgcaattgta gattgcccac gccatagccc
     2221 tgcgtcatca taaagttcga ttttatatga tttctgattc ccatttgcag aagttgaagc
     2281 cccacgaatt tgaatagtgc cattaggaac cgaaatgtta taacctagct caccaggcaa
     2341 gggtccggtc tcatctccaa tttgaaaaat aacttcagct tgtccaacca catcatttgc
     2401 cgcacgtgag cctttattcg tccacttatc aaaattgttt acctcttccc aagtgtactc
     2461 tgtgttttca agagaatttc ctttacgaac ggttacatac atacatacaa cagaagttgg
     2521 gtcatcattt tcgtacaaag ccaaattatc cttaatgggc tgattattga catcttcaac
     2581 tgaagtaatg ttaacaagtg tttgagaggg tgttgcagga gaaatgggtg tattatttga
     2641 tttgttaatt tccactgatg ttaaggattc agaaagactg ttcaaacttc caaacaaaag
     2701 aaatgtaaca ataatactta aagtatagtt agaaaatttt ttcattcaaa cacctgatta
     2761 ttagtagtaa agtaaaacac ttttatccca gggtgtattt ctgaatttgc atcagtataa
     2821 tagttataac tatattgaac cttccccaaa gaattaatgg cagtaacgcg ccaaaagtat
     2881 ttacctgggg ctagtaaatt caatggaact tcaatatttg tatcttttgt actcttctga
     2941 tacaacacct ctttaaatgc ccaatctttt cctagaacaa actgatatgt aagatccctg
     3001 ctcccaagat cataagagct tccccacacg aaatctacgt tatcgccatt aataataggt
     3061 gcctccataa caaacggcat aggcgtctct aaagattcaa catacaattt ataattattg
     3121 tctatatcag aagctaataa attcgcaatt tgatgtgaaa tgtttatatc aacgggcata
     3181 tgaaaagcat ctggcatagc aaaagaatat ttatcagtaa caggctgata tctctggaca
     3241 aaagcgttaa taacgtctga atttaaataa ccaagcactt cgtctatttt tttatctaat
     3301 ttattttggt attggtcatt ttctaaaact ctgcgatgca aagttgaata ccaatagtta
     3361 gacacacctc tctgccatgg taaataagtt ttggttgcta acttagcatt tgattgcaat
     3421 ggaaaagcct tatcgtaatc ccatggaata aagtaccact tatctccatt tttaggacta
     3481 tataagaagt aattctgaga aactgcctca acattccctg ttaatatgtt aaacgccatc
     3541 caagtaaagt aattctcaga atcaaaatac ttttcaaatg tattttcaat tggaatatca
     3601 gaattattta gctcatctag cattcttatt aactttgaat gatcttcgtc cccttttatt
     3661 tcaagtactt gtgaaaaagc atcttcacta tataaagggt ccgttacaag gtgtatctga
     3721 tcagggtatc gattaaataa aaaattatta gctttatata attgcccatt actatccaat
     3781 agacggctat ctaagaaacg ccgattaggt tgttcaactt gcgtaaacaa tccgtaatca
     3841 ataaacccct tttgaccttc aggtgattca atttttacat ataaatgaat aaattgtgtt
     3901 cttaatgtaa ctaaatgagg gattcctttc atcaaatcaa agctaagttt atttcgaaag
     3961 cgcgtctcat ctgacaagtg cttatttaag ttaataatac tttgtccacg ccatgcaccg
     4021 gaactattat caagggttag tttaaaagat ttctgcgaat caaaagcaga gtttgcacct
     4081 cgcagctcaa caagagcatt aggagttata tcattaaacc caaactctgt tggcagcgga
     4141 cctttatcgt cgccaaattg gactatagct tcgacatttt ttttatccat attggtatta
     4201 tttgttgcag ctagggtttt gtattcattc attgcagccc atgtttctgc atcgttttcc
     4261 ataactgtga taaagattgt cacaacagaa gaagaatcat catttatata tatatcagcg
     4321 ttatcttgaa aaattgactg gtcaacagag atgttgttct cggtaagcaa ttcctgttgc
     4381 gtagcaacaa gtttagaaac tgttaaattt ttatcaatac tagaatttgg agataaacta
     4441 agtaaagaaa ttatgattac aacaaaaacg gcaataatca ttaatactgc ccgaaaaaat
     4501 gctttcattt tttcacctcg atcaaatcta attgattggc tattctagta aaaaaacttc
     4561 ttttttgaac aaaaaacacc ggctgctttg taaaagtgtg gtagtcgagc ttatctgtat
     4621 aaagatacag agatcttaaa gaaactatat ataaaactac tgcagcaagt acaaagccaa
     4681 aaccatatag cgtttgagat gtcttaatag tgtaccaagt aatcaaaaca tttgagatgc
     4741 aaaaaagcgc agacgaccaa agcgcatcct gattatctga aaaataaagg agaaataaaa
     4801 ttaacacatt tccaatggcg taaataccat atcctacaca gagaactcga aacatctcct
     4861 gcattcccaa agtaaatcct aaattgagat gtattagaaa ttctcccaat agtgtaatag
     4921 ctaataatgt gaaaaacacc tgctgaattg ctaagtaaaa tatttccctc ttaagaacat
     4981 ctatcatatc attatatgct gtttcaatat tacttaaaga gccctctccg tttagaagat
     5041 tgaaataaag cttgtatttt gggtaaaaat tgacctcaac agtagcaaca aaattcacag
     5101 cactaacaag gcttgtggtg aatgctagta gagcaggtat atcatgacga ggtgcatgat
     5161 aaaaaagtcc ttcaacctgt actcctaatg ggctaaacca catcagcata agatggataa
     5221 aaagtccgaa cgtagaaaaa aaaccaatga aaggaagatg cggatatttg tcaatccact
     5281 ctaagaatct cagagaacta ccactaccca taggaaaaaa ctgatgaatt tctatcgtaa
     5341 aagctaccac taaaatgcca taggctatac atgctcccaa aagcaatgaa gataccactt
     5401 caaagttcaa caagataaat atataaccaa atgccaatcc actaaatata gaaacagaaa
     5461 atcctattaa tattccttta tattctttta aggcagagat gtaaccaatt tgcacccaag
     5521 caacaatccc ctcacagaat aaaataaatg aatacacagc atacgaaaat tgtattccac
     5581 taaaagcaag gaaaatgccc cataacacag aaccgacaac aagaaataat gaaatcgccc
     5641 catacatcga tggcaaaata cgtccatatt cttttatata catcatatca gcaacaaacc
     5701 tagtaagaac atataataat gtactagtca ataacaaaga gaataaaagt gagtatgtaa
     5761 tgatcacaac aatcaaatcc tgctgatgaa tagttgcatt cccgaactca gctacatacc
     5821 gcgttcccaa aagaaatatt gcgccaagca acattggacc tgcaaccacc atactggtat
     5881 atgcattggc tctaagttgt aataaaatgc cttttttcgc aaataacttt tttaattcga
     5941 agccgattcc tgccatttgc taatcgccct ttcgtataca cttaaataat tttttttcat
     6001 atcgttaata tcaaaaaact ttattgtacg ctgttgtcca gccatcccca tttgctttct
     6061 attatcttct gaatggcaca tttccattaa tgcttggaac aacttatact gatgcattgg
     6121 cgggacacaa ataccagctt gtccaaactt atcaccttcg tcaccctcaa ggagttcgcg
     6181 gcaacaaccc acatcggtac taacaactgg gcgtcttgca gccaaagact ctaaaactgc
     6241 aaagggttgc ccttcagaaa tacttgttaa aatcgtaaaa tcaatcttct caagataact
     6301 agttgtgcgg actgttccag gaataataat atctcttaaa tcaaggtact taattaactc
     6361 caaacactct tgatgatatt cctcgtcatc tataccacca ataatatgta aacgggcatt
     6421 atcaatttcc tgttttaagc gagagaaagt atatataagg gtcttaatgt ctttaattgg
     6481 cgcaaaacgt gcaataatgg caatatcaat ccaatcatct ttaggctttt ca
//
LOCUS       DAOQOS010000208         6476 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_6857_length_6476_cov_6.429995, whole genome shotgun sequence.
ACCESSION   DAOQOS010000208 DAOQOS010000000
VERSION     DAOQOS010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 6476)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6476)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6476
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6857_length_6476_cov_6.429995"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3042)
                     /locus_tag="PLF66_25045"
     CDS             complement(<1..3042)
                     /locus_tag="PLF66_25045"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HMZ67379.1"
                     /translation="MLYKYRKQIISTIVGVFLLWGIYQLLKPDTSIRIAVVGPMSGKD
                     AANGKSYLDGVKLYVNALNEKNGLDGRKIIVDVYDDENDKDKAKLKAEEIVKKNNVLA
                     VIGHNSSSCSIAAGPLYQANGIPAISPASTNPKVTIGNEWYFRTIFNDLTQGRFLANY
                     TKKVLKEPTFAILHDDKDYGSNLAKVFKETMSDHGQDPEYVWTIKTNNNENPEQELAA
                     IVEDIKSKEYTGLIFLATSPKDGAKFIEKFKDAGLSNRILAPDSFASNAFKESFAKLK
                     KEIQSPGYYSNDVMVTSPLIFDNANEKAQNFKDKYKAAYKAEPDWRAAYAYDSVMVLL
                     DVIHKIPDLKGGKANLKEDRLKVKEALSHLTNIDEAVEGVTGYNYYDENRDSPKPISI
                     GAYKNNDVISSLIQLQAVKNLKEITSIKDALEKETIIKIDNNYLYKTNVVYTGIEINE
                     ISKLDVSNLTYHMDFYIWFRFKEDIKPEDIEFLNSIEPITLGEPVDKDSSEHMNYRVY
                     HVKGNFKADNLPVRYELGYHAIGVNFKHKHLTRNNLIYVIDVIGMGMNSKKGDNKKKY
                     QSILSPVSGWMIANFWSFQDTMSRASLGAPKYLNSRDAKAEYSRFNLGVRIRKNEFTI
                     RGFIPGSHAGYYLGFSVLVVVGLGIAGRLKMFQKYSNLNWVITTLFIFLFLIASEIET
                     VDYLLDDLNTYSIKMVTLGFDLLWWIIPAMRLNQAIERFIWDPLEIKTGNKIPTVVRL
                     FLGIFIYILTFFGIVAFVFDQKITSLLATSGVVAMIIGLAIQVNISNVFSGIAINLER
                     PFKVGDWVKIGTLDEGKVIDITWRTTRIQTRNMIILSIPNSKASESPISNFGSKEDIT
                     EFWFTIHIDPNENTKRVQKILLDALLSAEGVLKNPAPYTRLNEFTEWSADYIIGYCWK
                     DYGRKNAVRKAVFTSIWTHLYRAGIQPATQRHEIHMFKGVKPRGEKAQSPLAVLQELD
                     IFSSFTEKEKKYLSTHSKTHIFKPGDAIVHTGDTNQSLFVIA"
     gene            complement(3134..4036)
                     /locus_tag="PLF66_25050"
     CDS             complement(3134..4036)
                     /locus_tag="PLF66_25050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67380.1"
                     /translation="MNNVSRIFLSLQMILVSSCYVIPDNPFSARLQCYKKNYCDTAVN
                     DCIVGSALFGALFRSPTSSSTSTTSTSSTTPVESLTNASKIGTTSSSTFSNPQTYTLA
                     FSSSSTLTTPYNSANYSTSTPTYQNLNVTGTLVTPNNIDIMSMSYTSVGYTPGSGSYT
                     CIGYTGNTRIVEVSKISGSATCQLYSQFGASTNATTTATTETLVGTLTSTVTTFTFNI
                     STGPTYLYVRCSGVTNDQYAVRFAYSSSNKNNEVFPCTSSTSSSSSSSSSFSSNFLNY
                     LTSPLTCVNADKDCRSDCDRKHIY"
     gene            4328..>6476
                     /locus_tag="PLF66_25055"
     CDS             4328..>6476
                     /locus_tag="PLF66_25055"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HMZ67381.1"
                     /translation="MSNITQKKPSLLGKLIKKFNQSSFEIIFFSLTFLVLSVMGVTGF
                     TYYKNSTAILSLTEDLIDQINNRVIQKTTSYLFTAVDMTELSSKVAGEGANSILDNQG
                     LNSLMINILILHPQLTMFNIGNERGDFLMQKRWTDGTIATKTIDRSKKDPVVKWKYRD
                     QTGKIIKEEVVQEDYDPRKRPWYIGAKEKSGLFWSDVYIFATGKIPGITASAPVKDGE
                     GNLMGIFGLDIPLIEISNFLEDLRKEIQDKKYGKTAIAFIVNSKGDLVAYPDVTQPMI
                     KGEDGNLRSRKVKELEVNPPIVQDSYEKFIQEKQTKFTFETGGKRYIASYKKFPDNFE
                     KDWTIGLVVPEDDYIGAIKETNDLMLTISIVILCIAMVLALILNRIKKALNARNEFIK
                     NTFGRYLSDDVVASILESPKGTSLGGEKREVTIMMTDLRGFTSISERLPAEKCVLMIN
                     NYLDVMTDVILKYQGTIDEFIGDAILVIFGAPIQREDDAARAVACAIEMQLAMKEVNE
                     KNRRDGLPEVVMGIGLNTGEIVVGNIGSHKRTKYGVVGSNVNLTSRIESYTVGGQTLV
                     SQRTLEACGPIVRVDDQFEVMPKGVKNPITISEVGGIGGEYNIYLPEKKEIVLHPLKR
                     KIQFLFTILAGKHASTDVHVGYITKMNGKQAEIEAPISVEKLNNVKVALADENGKEIA
                     NDLYAKVTKNVSESPKVIFQVNFTSVPTEVEKVF"
BASE COUNT         2126 a         1130 c         1141 g         2079 t
ORIGIN      
        1 tgcaatgaca aagagagatt gattagtatc ccctgtgtga acaatcgcat cgcctggttt
       61 aaagatatgg gtcttggaat gagtgctgag atatttcttt tctttttctg tgaaagaaga
      121 aaatatatcg agttcttgta aaaccgcaag tggagattgt gctttttccc cgcgaggttt
      181 aacccctttg aacatgtgaa tttcgtgacg ttgtgtggca ggttgaattc cagctcggta
      241 aagatgagtc caaatactag taaatactgc ttttctaacg gcatttttac gaccatagtc
      301 tttccaacaa taaccaataa tataatcagc agaccactct gtgaattcat tgagtcgtgt
      361 gtaaggagca ggatttttta atacgccctc tgcggataat aatgcatcta gtaaaatctt
      421 ttgcactctc tttgtatttt catttggatc aatatggatt gtaaaccaaa actcagtaat
      481 atcttctttc gatccaaaat tagaaatagg tgattccgat gctttactgt ttggaatact
      541 taaaataatc atatttcttg tttgaatacg agtggttctc caggtgatat cgatgacttt
      601 tccttcatct aaagtcccaa tttttaccca atctccaacc ttaaaaggtc gttctaagtt
      661 aatcgcaata cccgagaaca cgtttgaaat atttacctga atagcaagac caatgatcat
      721 tgcaaccacg cccgatgtag cgagaagtga agtaatcttt tgatcaaata cgaaagcaac
      781 aattccaaag aacgttagta tataaataaa tatacctaga aaaagtcgaa caacagttgg
      841 gatcttgttt ccagttttta tttcgagtgg atcccaaata aatctttcaa ttgcttgatt
      901 gagtcgcata gcagggatta tccaccaaag taaatcaaac cctagtgtaa ccattttgat
      961 cgaataggta tttagatcat ctagcaaata atcaacggtt tcaatctcag atgcaattaa
     1021 aaataaaaat ataaatagag ttgtaataac ccaatttaaa ttagaatact tctgaaacat
     1081 cttaagtctt ccagcaattc caagacctac gactactaga acgctaaatc ccaaataata
     1141 accagcatgg ctgcctggaa taaatccacg tattgtaaat tcattttttc gaatgcgaac
     1201 tcctaagtta aatctagaat attctgcctt tgcatcccgg gagtttagat atttaggagc
     1261 acctaaggaa gctcttgaca tcgtatcctg aaaggaccaa aagttagcaa tcatccaacc
     1321 tgatactgga cttaaaatac tttgatattt ctttttatta tcgccttttt tagaattcat
     1381 tcccatccca atcacatcaa tcacatagat cagattgttt cgagtaagat gtttatgttt
     1441 aaagttcacg cctatcgcat gataccctag ttcgtaacga acgggaagat tgtctgcttt
     1501 aaaatttccc ttcacatggt aaacacgata gttcatatgt tcactactat ctttatccac
     1561 tggttcgcca agagtaatag gctctatgct atttaaaaat tcgatatctt ctggcttgat
     1621 gtcttcttta aatcgaaacc atatataaaa atccatatga taagttaaat tggaaacatc
     1681 taatttacta atttcattga tctcgattcc agtatatacg acgttagttt tatataagta
     1741 attattatca attttgataa tggtctcttt ttcaagagca tccttgatac tagtaatttc
     1801 ttttaaattc ttaacagctt gcaattggat caaagaggaa atcacatcgt tatttttata
     1861 agcaccgatt gaaataggct ttggagaatc tctattctca tcataataat tgtaacccgt
     1921 caccccttct actgcttcat ctatatttgt taagtgagaa agagcttctt ttacttttaa
     1981 gcgatcctct tttagattcg ccttaccacc ttttaaatca ggtattttat gaattacatc
     2041 aagtaaaacc ataacggagt cgtatgcata ggcagctctc caatcaggtt ccgctttata
     2101 agccgctttg tatttatctt taaaattctg tgctttttca tttgcattgt caaagatgag
     2161 aggacttgtc accatcacat cgttggaata ataacctgga ctttgaattt ctttttttag
     2221 tttagcaaaa gactctttaa atgcgttaga tgcaaaagaa tcaggagcaa gtatacgatt
     2281 agagagacct gcatctttaa atttctcaat aaactttgct ccatctttag gagatgttgc
     2341 aaggaagatg agtccagtgt attccttgct ttttatatcc tccacaatcg cagccagttc
     2401 ctgttctgga ttttcattgt tgtttgtttt aatagtccat acatattcag ggtcttgtcc
     2461 gtgatcgctc atagtttctt taaaaacttt tgccagatta gatccatagt ctttgtcatc
     2521 gtgtaggatc gcaaaggtag gctcttttaa aactttctta gtatagtttg ctaaaaatct
     2581 tccttgcgtt aaatcgttga atatagttct aaaataccat tcatttccaa tggtcacttt
     2641 tggattcgta gaggcaggtg atatggctgg aattccattc gcctgataaa gaggtcctgc
     2701 tgcaatagag caagaactgc tattgtgacc aattactgcg agaacattgt ttttcttaac
     2761 aatttcctct gcctttagtt tcgccttgtc tttatcgttc tcatcatcat atacatctac
     2821 tataatcttt ctgccatcta atccattttt ctcattcaaa gcatttacat atagcttaac
     2881 accatctaga taggattttc cattggcagc atccttgcct gacataggac ccactacagc
     2941 aattcgaatg cttgtatctg gttttagtaa ttgataaatt ccccaaagga gaaatactcc
     3001 aacgatcgtg gaaataattt gttttctata tttgtatagc ataggcgttt agcttaacca
     3061 taacaaaaaa tttcgctacc tgaacaagtg caaaaatatt tacacttgtt aattattagt
     3121 ttgaagggtc tttttagtaa atatgtttcc tgtcacaatc tgaacgacag tccttatccg
     3181 catttacgca agttaatggt gaggtgaggt aatttaagaa attactagaa aatgaactac
     3241 tgctactgct gctagaagat gtgctagaag tgcaaggaaa aacctcattg tttttgtttg
     3301 agctagaata tgcaaatcga actgcatatt gatcgtttgt cacaccagaa catctaacat
     3361 acaaataagt aggacctgtt gaaatattaa aggtaaaagt agtcacagta gaggtaagag
     3421 ttccaactaa agtttccgtt gttgccgttg ttgtcgcatt ggttgatgct ccaaattgac
     3481 tgtaaagctg acaagtagcg gatccagaaa ttttagaaac ttccactatt cttgtattgc
     3541 ctgtataacc aatacaagta taactaccac ttccaggcgt atatccaacg cttgtataag
     3601 acatactcat tatatcaata ttattggggg taactaaagt tcctgttaca ttgagatttt
     3661 gataagtagg tgtagaggta ctgtaattcg cactattgta aggagttgtt agtgtagaag
     3721 aggaagaaaa cgccaaggta taagtctggg gattactaaa agtactactc gaagtagttc
     3781 ctattttact ggcattggtt aagctttcca caggcgtagt gcttgaggta cttgttgtac
     3841 ttgtagatga gcttgttgga gatctaaaaa gcgcaccaaa taaagcagaa ccaacgatac
     3901 aatcatttac agctgtatca caataattct ttttataaca ttgcaatctc gccgaaaaag
     3961 gattatccgg gatcacatag caacttgaaa ctaatatcat ttgcagggat agaaatattc
     4021 tgcttacatt attcattttt tttctccaat ttttaaccta aatctgtttt tttgtattta
     4081 actaggttgc tatttctgaa aaatccatgt caataactaa atttttctat actatttaaa
     4141 atataatatt tctaagaatt ttccaaggag attaaagata ttctattctc tttttgtttt
     4201 tgctttttta cttaaattaa gattgtcatt ttttatttta ctagaaaact aacaaaacaa
     4261 tattcgaaag atacattcaa tacgatatta aagaatagct ttcctttatt ggcaggagtc
     4321 catttacatg tctaatatta cacagaaaaa accaagttta cttggcaaac taatcaaaaa
     4381 attcaatcag tctagctttg aaattatatt ttttagtctc acttttcttg tactttctgt
     4441 gatgggtgtt acaggtttta cctactacaa aaactctact gcaattcttt ccttaactga
     4501 agacctaatt gatcaaatca acaatcgtgt gatccaaaaa acaacaagct atctttttac
     4561 tgctgtagat atgacagagc tttcttccaa ggtggctgga gaaggagcaa attcaattct
     4621 agacaatcag ggtttaaact ctctgatgat caatatctta atcctacacc cacaactcac
     4681 aatgtttaat attggaaatg agagaggtga ttttctcatg cagaaaaggt ggaccgatgg
     4741 aactatcgct actaaaacaa tcgatcgatc taagaaagat ccagtcgtca aatggaaata
     4801 cagagaccaa acgggaaaga ttatcaaaga agaagtagtt caagaagatt acgatccaag
     4861 aaagagacct tggtacattg gggcaaagga aaaaagtggt ctcttttgga gtgatgttta
     4921 tattttcgca acaggcaaaa ttccaggaat tacagcatcc gctccagtaa aagatgggga
     4981 aggaaatcta atgggaatct ttggactaga tattcctttg attgagattt caaacttcct
     5041 agaagatctt agaaaagaga tacaagataa aaaatatgga aaaactgcca ttgcatttat
     5101 cgtcaattca aagggagatc tagtagctta tccagatgtt acacaaccaa tgatcaaagg
     5161 agaagatggg aacctaagat caagaaaagt aaaagaatta gaagtaaatc caccgatcgt
     5221 gcaagattct tacgaaaaat tcatacagga aaaacaaacc aaatttactt ttgagacagg
     5281 cggcaaacgt tatatcgcat cttataaaaa gtttcctgat aattttgaaa aagattggac
     5341 cattggtctt gtagttcctg aagatgatta cattggagca atcaaagaaa ccaatgattt
     5401 aatgctcaca atttccattg tgattttatg tattgcaatg gttttagcat taatactcaa
     5461 tagaattaaa aaagcactca atgctagaaa cgaatttatt aaaaatacat ttggacgtta
     5521 tctttcagat gacgttgtag cgagcattct agaatcgcca aagggaactt ctcttggagg
     5581 agaaaagaga gaggttacta taatgatgac cgacttacgc ggctttacct ctatcagcga
     5641 aagacttcct gctgagaaat gtgtcctaat gattaacaat tatttggacg ttatgactga
     5701 tgtaatttta aaataccaag gaacgataga tgaatttatt ggggatgcaa ttcttgttat
     5761 ctttggagca ccgattcaaa gagaagatga tgcagctcgt gccgtagctt gtgcaattga
     5821 aatgcaactt gcgatgaaag aagtaaatga aaaaaatcgt agagacggtc ttcctgaagt
     5881 ggttatggga attggtttaa atactggaga gatcgttgtc ggaaatattg gttctcataa
     5941 acgaacaaaa tatggagttg taggaagcaa tgtaaacctt acatctagaa tcgaatctta
     6001 tacagtaggt ggacaaaccc ttgtatcgca aagaacttta gaagcatgcg gtcctatcgt
     6061 tcgagtggac gatcaatttg aagttatgcc taagggagta aaaaacccaa ttacaattag
     6121 tgaagtaggt ggaattggtg gagagtataa tatctattta cctgagaaaa aagaaatcgt
     6181 tctgcaccca ctcaaaagaa agattcagtt cctatttaca attttggctg ggaaacacgc
     6241 aagcacagac gtgcatgttg gctatattac caagatgaat gggaaacaag cagaaattga
     6301 agctccaatt tccgtagaaa aattaaacaa tgtaaaagtt gcactcgcag atgagaatgg
     6361 aaaagagatt gcaaatgatc tctatgctaa ggtgacaaag aatgtttctg agtcccctaa
     6421 ggtgattttc caagtcaatt ttacatctgt tcctacggaa gttgaaaaag tattca
//
LOCUS       DAOQOS010000209         6457 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_6884_length_6457_cov_6.821150, whole genome shotgun sequence.
ACCESSION   DAOQOS010000209 DAOQOS010000000
VERSION     DAOQOS010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 6457)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6457)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6457
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6884_length_6457_cov_6.821150"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..283
                     /locus_tag="PLF66_25060"
     CDS             <1..283
                     /locus_tag="PLF66_25060"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HMZ67382.1"
                     /translation="FVPTPREPVSINDMKIWAVKASALACENLMLALRAFGYDSCPLE
                     FHDSSRIKKLLDLSSDAIITMVITAGKRAPNGLYGDRTRLDKLHFIREV"
     gene            complement(287..1885)
                     /locus_tag="PLF66_25065"
     CDS             complement(287..1885)
                     /locus_tag="PLF66_25065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015680069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1957 domain-containing protein"
                     /protein_id="HMZ67383.1"
                     /translation="MNSKVGYQILVLHAHLPYVRHKGYSPAFLEENWLNEAISETYLP
                     LIKSFRTLKREQVHFKITMTFTPTLLSMLSDEYLRDKFLAYTENLIELSEKEVKRTTY
                     DPHLNYLSRFYLENFEDNRRLFLELKKDVVEGFREFVKDGSLEVITCPATHGFLPLLE
                     SEPSSVHAQIHIGRKTHKKFWGLEPRGIWLSECAYYPGVEKYLADEGIRYFFVDTHGI
                     LNATPRPRYGVYAPVEVGNGVFAFARDKESSAQVWSSLHGYPGDFRYREYYRDIGYDL
                     DYEYIKDYVHPTGIRLNTGIKYHRITGKTDYKDYYHPDWAIEAAGNHAEDFLRSRIAQ
                     SEKILHEEGQPAVIVSPYDAELFGHWWYEGPKFIEFLFKKMHFDQDKIQAVHPMQILG
                     LIPRIQSVKMDMSSWGDGGYSDVWLNHGNEWIYRHIMECCIIMDQTVHLHHNTEDKTI
                     RRIVNQMARELLLLQSSDWPFIIRMGTMVDYAQKRIRIHAGLFFELTEMLKPNGLDEA
                     RLKEIEEEHPIFPDIRFEDFIWKK"
     gene            complement(1875..2531)
                     /locus_tag="PLF66_25070"
     CDS             complement(1875..2531)
                     /locus_tag="PLF66_25070"
                     /inference="COORDINATES: protein motif:HMM:NF027583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4912 domain-containing protein"
                     /protein_id="HMZ67384.1"
                     /translation="MTEEKKSRIYLDPKFDRLPDYYNRDIVKILTKNLKEAYVFWGIS
                     TESFKKILDTFNCSKNEVYFKLIVRYAQEDKIHRHKEIFLPPFTNNWFLEFDQRIKNL
                     KVEVIAFSQAGHSYSLLHSAEISIPSHKPSKVLHRDWLKASWIKEFNLVDVNGEIHIQ
                     DGPKQEPNSNTITINQSLSEELEWQEGFFTWPDGSSGFMGSSGIFASSSNHMRKKNEQ
                     "
     gene            4137..4439
                     /gene="raiA"
                     /locus_tag="PLF66_25075"
     CDS             4137..4439
                     /gene="raiA"
                     /locus_tag="PLF66_25075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004750477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HMZ67385.1"
                     /translation="MKIKYTWKHLDHSAAAEEYATKKLESINKYLHKITSCDVSFHMV
                     HGEIHINMNVRGDQSTFNAHNINKDIYACIDGLEDKIERQLNRFHDKKAAHSKTLR"
     gene            4440..5384
                     /locus_tag="PLF66_25080"
     CDS             4440..5384
                     /locus_tag="PLF66_25080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67386.1"
                     /translation="MLVRFEEGNFRLKEGEFENAKNIFFELLDIDPNKQEFIAGYYIS
                     SYWDNRIEMILAAREGKERGNLLVEMFNQFAGEITKRNFPKNETYESVTFCILSEAST
                     QFRIAYQKEGMNGLDRNVLSNLAMCLIRIGDYKNAKDILDYSKKFQDLSMQNLYYEAE
                     CLYHLGDHSRSRLYYRIAMLNNPDLLNLEIVKSEPLATAILELGEQFSSMDEIREIIP
                     VYCLEKHLITEIKDYTREEVNQLFLEMMRLESNLNTEKPELKFKLQCRILHIGLTILD
                     TFQGQINTELSKKVKNKINMIDPEILDRRIDNKREKGL"
     gene            complement(5387..6445)
                     /gene="dinB"
                     /locus_tag="PLF66_25085"
     CDS             complement(5387..6445)
                     /gene="dinB"
                     /locus_tag="PLF66_25085"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF010731.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HMZ67387.1"
                     /translation="MHIDMDAFYASVEQRDFPEWKGKPVVVGGDPNSRSVVSTASYEA
                     RKYGIHSAMPTSKAKRLCPHAIFVYPRFSAYKQVSREIHEIFHEFTDLVEPLSLDEAY
                     LDVTENKIGNPSATLVAKEIKKRILERTQLTSSAGVASIKFIAKIASGMNKPNGLTVI
                     TPDEAEKFLENLPIGKFYGIGEATERKMISLGIKTGLDLKQFTQSELIRHFGKSGSFY
                     YNIVRGNDFRKVEPYRERKSIGAENTFSIDLLERSAILAELSDISDVMWDRVERIGTK
                     GKTLTLKVKYDNFDLISRSISFQTYISSKAQVDQFAKELLNNTEVGKRKIRLLGLTIS
                     NLESHEEEDSGQLTFEFK"
BASE COUNT         2078 a         1051 c         1100 g         2228 t
ORIGIN      
        1 atttgttcct actcctaggg aaccagtgag tatcaatgat atgaaaatct gggctgttaa
       61 agcgagcgct cttgcctgtg agaatcttat gttagccctt agagcttttg gatacgatag
      121 ttgtcctctt gagtttcatg attccagtcg tatcaaaaag cttctagatt tatcttctga
      181 tgcaattatc acaatggtta tcaccgccgg taaacgtgcg cctaacggct tgtatggtga
      241 tagaactcgt ttagataaac tacactttat tcgagaagtt tagtttttac ttcttccaaa
      301 taaaatcttc gaatcgaata tccggaaaga taggatgttc ttcttctatt tcttttagtc
      361 ttgcttcgtc tagtccgttt ggttttaaca tttctgttaa ttcaaagaat agacctgcat
      421 gaattcgaat tcttttttgt gcataatcta ccatggttcc cattcgaata ataaacggcc
      481 agtccgaact ttgaagtaaa agtaactctc ttgccatttg gttcacaatt cgacggattg
      541 ttttatcttc tgtgttgtga tgtaaatgga cagtctgatc cattataata caacattcca
      601 tgatatgtct gtaaatccat tcgtttccat gattgagcca aacatcagaa tatccaccat
      661 ctccccaact agacatatcc attttgacac tttggatacg tggtattaaa cctagaatct
      721 gcattggatg aactgcttgg attttgtctt gatcgaaatg cattttctta aataggaatt
      781 caataaattt aggaccttca taccaccaat gaccaaataa ttcggcatca tagggagaaa
      841 cgataaccgc aggctgtcct tcttcgtgta agattttttc tgattgtgca attctacttc
      901 gcaagaaatc ttcagcgtga tttccggcag cttcaattgc ccaatctgga tgataataat
      961 ctttgtagtc tgtcttgcca gtgatacgat gatatttgat tcctgtatta agacgaattc
     1021 cagttggatg gacgtagtct ttaatatatt cataatctaa atcataacct atatctcgat
     1081 agtattctct gtaacgaaaa tctccaggat atccatgaag agaactccaa acttgtgcgg
     1141 aactttcttt atcacgcgca aaggcaaaca cgccgttacc cacttcgaca ggagcataga
     1201 ctccataacg aggtctagga gttgcattta aaataccatg agtatcgaca aagaaataac
     1261 gtattccttc atctgctaaa tatttttcga ctcctggata gtaagcacat tctgaaagcc
     1321 atattcctct cggttctaaa ccccaaaatt tcttatgtgt ttttcttccg atatgaattt
     1381 gtgcatgaac agaagatggt tcactttcta gtaatggtag aaatccatga gttgcaggac
     1441 aagtaataac ttctaatgat ccatctttga caaattctct aaaaccttct actacatctt
     1501 tttttaattc taagaataat cttctattat cctcgaagtt ttctagatag aatctagata
     1561 ggtagtttaa gtgtggatca tacgttgtgc gttttacttc cttttctgat aactcgatta
     1621 gattttcagt gtaagctaaa aatttatctc ttagatattc atctgatagc atagagagaa
     1681 gcgttggagt aaaagtcatt gtaattttaa aatgaacttg ttctctcttt aaagttctga
     1741 aagatttaat taaaggtaaa taagtctctg agattgcttc atttaaccaa ttttcttcta
     1801 agaaagctgg cgaatatcct ttgtgtctaa cgtaaggaag atgagcgtgt aatactaaaa
     1861 tttgatagcc gactttactg ttcatttttt ttcctcatat ggtttgatga tgaagcgaag
     1921 attccagagg atcccataaa tcctgaggac ccgtctggcc aagtaaagaa gccttcttgc
     1981 cattctagtt cttctgataa agattggttg attgtaattg tgtttgaatt cggctcttgt
     2041 tttggtccat cttgaatatg aatctctcca tttacgtcta ctaggttgaa ttctttgatc
     2101 caagatgctt ttagccaatc tctatgtaga actttactag gcttatggct tggaatcgaa
     2161 atttctgctg aatgcagtaa actgtaacta tgacccgctt ggctaaatgc aattacttct
     2221 acttttaagt ttttaatacg ttgatcaaac tctaagaacc aattgtttgt gaaaggtggt
     2281 aaaaagattt ccttgtgtct gtgaatttta tcttcttgtg cgtacctaac gattaattta
     2341 aaatagactt cattcttact acaattaaaa gtatctaaaa tttttttgaa actctcagtg
     2401 ctgattcccc aaaaaacata ggcttctttt aaattcttgg taagtatttt tacaatatct
     2461 ctgttataat agtctggaag tctgtcgaat ttaggatcta aataaattcg ggattttttt
     2521 tcttcagtca tgagattatt cctctctatt tttacatagc agtcagttta cctttattgt
     2581 aaatgatttt tatatctata tgattttgat tttatatgag atttgtcatt agaaacttat
     2641 ttatacagag tccgatggaa aaaggcattt tattaaggaa aaatttttct aattgaatta
     2701 atttctatag aatcttgcaa ttttaaccct tttacagtta cttgtgtagt gtaatacgct
     2761 tgtttctttc tactatttgt cattaaatgc aatgttttga tttgttttgc caacctgtag
     2821 agtatgttta gatgtacaat tacagcaaat aggatttagt ttaagtaaaa cgataaatgg
     2881 aagagtgggc aatttctgca tagttttttg atatatttcg tctcgactgg gctaggatca
     2941 aaggttaatt gttttatagt ctcaaattat caatcaatta gaaaaatatt cagattgcaa
     3001 agtcagagtt agtaacaaac ctaacgcaaa atttatgaat aaagttgaat agaaacagtt
     3061 ttagaaaaag aattagaatc cgaataaacg aataaaacca atagatttcc ttcctttagg
     3121 tgacaggaaa gcaagaaaat gatgtgagaa aacgcctatc tacagatacc tatttctttt
     3181 ttgaatgaca attaggcaaa attgctttga tttaaaagca gtcattcgtt ttgagaatca
     3241 ggattaatag cgagcgtata ggatgggtaa gtatgtcatc gactagaaaa ttcttttttt
     3301 ctcttgtcta cagctatttt ctttaaggat cgttatagac tatttacggg gaaaatttca
     3361 ctaagaggtg gaactattta gagaacaaaa tgaataagat ttaaaaatct ggtattgatt
     3421 tttgttatga atagatggta tataattttt aaaaatttct agggggaatt tttaaaaaat
     3481 gatttagaga tgcgaatgaa atggtaaaat caatagcctg tgatcgctct tttgttagag
     3541 caatcataat gatggttaaa caagtcctct atttcttctc tttcactttt atcaaatgaa
     3601 atgacaataa gagaagagca agcctcattt gaatgttagg ttcactaaaa actcaatctt
     3661 tacctgtgtt atgcaatgat aaataatttg aatgattgat ggaaatttat aatctgacct
     3721 aatttatttt tgttagaaaa gatttacttt ctttagattc ttttaataca attcgtttgg
     3781 aatgcgttat tatacctatt aaggttttac tgaatcagaa aataaagtat tagaattaag
     3841 tattataaaa aagaaagagg ataattataa gttgcctctt cctttgaaat agaattaata
     3901 aataattgac tgatctatgt tggaattttt attaaaaatt aagatcggaa ttggatgaaa
     3961 tgagttctga aagaatttta ggcaagaaaa aattttaaag attatttaaa aaaataaaaa
     4021 tatatttaga tatatatgtg tactttttcg aaataatttc ggaatttttt cgatagtact
     4081 tgactataaa aatgtaggag ggtgaaaata ggcttgtaca aaaggagatt ggaactatga
     4141 aaattaaata cacttggaag catctagatc attcagctgc tgcagaagaa tatgcaacaa
     4201 aaaagctcga aagcattaat aagtatttac acaaaatcac ttcgtgtgat gtgtcttttc
     4261 acatggtgca cggggagatt catataaata tgaatgtccg tggagaccaa tcgactttta
     4321 atgcgcacaa tataaataaa gatatttatg cttgcataga tggattagag gataaaattg
     4381 agcgtcagct aaatcgtttt catgataaaa aagctgcaca ttcaaaaaca ctgaggtaga
     4441 tgctggttcg gttcgaagaa ggaaatttca gattaaaaga aggggagttt gaaaacgcta
     4501 aaaatatctt cttcgaactt ttagacattg atcctaataa acaggagttt atagctgggt
     4561 attatatatc cagctattgg gacaaccgaa tcgaaatgat tctagccgcc cgcgaaggga
     4621 aagaacgtgg caatctactc gttgaaatgt tcaatcaatt tgccggggaa atcaccaaac
     4681 gaaattttcc aaaaaatgaa acctacgagt cagtaacttt ttgtatatta agtgaagctt
     4741 cgactcaatt tagaattgcc taccaaaaag agggaatgaa tggcttagac agaaatgtcc
     4801 tttccaatct agctatgtgc ctcatccgca ttggggatta taaaaatgca aaagacattt
     4861 tagattattc taaaaagttc caagaccttt caatgcaaaa cttatactat gaagcagaat
     4921 gtctctacca tttgggtgat cattctagaa gcagacttta ttatcgaatt gcgatgttaa
     4981 ataatccaga tttgctgaat ctagaaattg taaaatcaga accactcgca acagctatct
     5041 tagaacttgg ggaacaattt tcttctatgg atgagattag agaaatcatt ccggtctact
     5101 gcctagaaaa acatttaatt acggaaataa aagattatac cagagaagaa gtgaatcaac
     5161 tcttcttaga aatgatgcga ctcgagtcca acctcaatac agaaaagcca gaacttaaat
     5221 ttaagttaca atgtagaata ttgcatattg gtcttactat tctagataca tttcagggac
     5281 aaatcaatac agaactttct aaaaaagtaa aaaataaaat caacatgata gacccagaaa
     5341 ttctagatcg ccgcattgat aataaaagag aaaaaggttt gtagtattat ttaaattcaa
     5401 acgttagttg accactgtct tcctcttcat gactttctag atttgaaata gtaagtccta
     5461 agagtctaat ctttctcttt ccaacttcag tattatttaa cagttctttt gcaaattgat
     5521 ctacttgtgc tttcgaagaa atataagttt gaaaactgat acttctggag attaaatcaa
     5581 agttatcata ttttactttt aatgtgagtg tttttccttt cgttccaatt ctttctactc
     5641 gatcccacat tacatcagag atgtcagaga gttcagcaag aatagcagac ctttcgagta
     5701 aatcaataga aaatgtattt tctgctccaa tggattttct ttctctataa ggctctactt
     5761 ttcggaagtc atttccacga acaatgttat agtaaaatga tcctgatttt ccaaaatgac
     5821 gaatcaattc actctgtgta aactgtttta aatccaaacc tgttttgatt ccaagagaaa
     5881 tcatttttct ttctgtcgct tctcctattc catagaattt tccaataggt agattttcta
     5941 aaaatttttc tgcttcatcg ggggtaatca ctgttagccc atttggttta ttcattccag
     6001 aagcaatttt tgcaataaac ttaatggaag caactccagc ggaggatgtg agctgtgttc
     6061 tctctaagat tctttttttg atttcttttg cgaccagtgt agcgcttgga tttccaattt
     6121 tattttctgt tacatctaga taggcttcgt ctaacgatag cggttctact aaatcggtaa
     6181 attcatgaaa aatctcatgt atttctcgtg aaacttgctt gtaagcagag aagcgaggat
     6241 atacaaatat cgcatgcgga caaagtctct ttgctttaga ggtcggcata gccgaatgta
     6301 ttccgtattt tctagcttca tagcttgccg ttgatacaac tgacctacta tttggatccc
     6361 cgcctacaac aactggtttc cctttccatt caggaaagtc cctctgttct acacttgcat
     6421 agaaggcatc catatctata tgaattattt ttcgaag
//
LOCUS       DAOQOS010000210         6448 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_6901_length_6448_cov_6.977475, whole genome shotgun sequence.
ACCESSION   DAOQOS010000210 DAOQOS010000000
VERSION     DAOQOS010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 6448)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6448)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6448
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6901_length_6448_cov_6.977475"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1168)
                     /locus_tag="PLF66_25090"
     CDS             complement(<1..1168)
                     /locus_tag="PLF66_25090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012388618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HMZ67388.1"
                     /translation="MLLIIFWGQIYNNLYSERSIHLGKVISEIKFIGNQNTSKDDILN
                     FIEMRKGMVLTEKLLDHDLKALFATGYFYNIDIKADTAANGGVIVIFEIKERPRVEEI
                     EFVGADEVFPSDLRDKMPIKENEVITPEKVNTTREVILKKYRDEGFFHAYVKFEMGKI
                     NPKTNLVKVKFIIDEGDEIPVSKINIIGAKAIDPGEIVGFLDLKEAGLIESGHFKEAS
                     FESDKQKIVAYLKSKGYLDADIVPEGTNWEIRWENAKLKDKRVIIVNYKIKEGDIYYF
                     NGYEAKHDYTLDERGNPIFLNKENNPEGTKKEDLKPIYSIKELEKLFEFSNDDIGDVF
                     DEGKFQRDKQTINELYSSKGYLYVQVMPKRKNIELTHENLLNYENSKRTQLAFRN"
     gene            complement(1225..1686)
                     /locus_tag="PLF66_25095"
     CDS             complement(1225..1686)
                     /locus_tag="PLF66_25095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004461042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HMZ67389.1"
                     /translation="MKFRRTMKTFDRIDITSLIDVVSFIVIYFLINATLEKGTSIKVE
                     LPRSSSSVTKEKAQDELIITVDKAGKITLDKDPTPVPLEQLKDKIISFLGPKKDRDPK
                     KNRVIIRGDSGANYQTVVKVIDEINAAGVTRFNLAMVKGKNSGNSEPAEDQ"
     gene            complement(1683..2303)
                     /locus_tag="PLF66_25100"
     CDS             complement(1683..2303)
                     /locus_tag="PLF66_25100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017213590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HMZ67390.1"
                     /translation="MFHLGKSNSIIASIPPETVPIIIIIVSIIAFTIIIERTVYFWRL
                     RPINPEDFRKVKDLLIEKKWDEAKDYLVSCSTGPTTAILVTGIEYKRRESKYFEDEMK
                     SEGYRQIFKMEKYLTGLGTIATIGPLLGVLGTVVGMIRSFAEGAGTKGAEVGISEALI
                     TTAMGLSVSIPAYIFYNYLVRKKEEKIAEMENLSDQFLQVFHKEKV"
     gene            complement(2369..3250)
                     /locus_tag="PLF66_25105"
     CDS             complement(2369..3250)
                     /locus_tag="PLF66_25105"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HMZ67391.1"
                     /translation="MKYFHSLLIPILFVSYTACASHNPRTKKVEEVIPQKFLVIGHRG
                     TGDNSIAPENTLSSFRFAAKNKSAFELDTMFAKSGELVVIHDSDVDRTTNGKGMVDSL
                     TLTELKQLDASKLSNPKFKGEKIPTLEEVLNEFGGKTLVDIEIKAQAPKEEAQRIGAA
                     VAKLVGSKKLEKQVFVTSFNPYVLETIKHTNPEILRGQLYSNFDDVKELSWIVKVVLR
                     NLLLNGKAEPDILAPREDMVNSDYIEKYHGLGYKIFPWTVNDPVKMEKLIKLGVDGLI
                     TDRPDLANEIYEKLKNQ"
     gene            3370..4011
                     /locus_tag="PLF66_25110"
     CDS             3370..4011
                     /locus_tag="PLF66_25110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67392.1"
                     /translation="MKKDYLLIIGGLVPLLFSLFVLNYFLKDDRKDLFESAKLLDSKS
                     YPNIKEGDFVKFSGILSQKNSFVKDKYVLAVKEKFVKGQNGKRSDWFREEFYFQTLFV
                     ESSGVPTFEINVANDYLPCGNEVQISDSIPKKSRVLGIISGVNVSAVGRVTSINPLRL
                     DTGHSLCTGSIEDYDSYLRKKNLGYLFILICIALPSLGLIYFGFFKERKIKPE"
     gene            4022..5053
                     /locus_tag="PLF66_25115"
     CDS             4022..5053
                     /locus_tag="PLF66_25115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020771177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HMZ67393.1"
                     /translation="MKEEIISKDELRKRLQEENFKRVTLSFYRYTKIENPNELRLQLL
                     SEWGNLNVFGRIYLAKEGVNAQLSVPEFNFEKFKAQLNSLEYSKNMPLKIAIEDDGKS
                     FFKLTIKVKEKIVADGLDDSSFDPSNVGKHLTAQEFNKALEDPNTVVVDVRNHYESEI
                     GHFEKAICPDADTFREELPMIVDLLQDKKDKKILLYCTGGIRCEKASAYLKHKGFLDV
                     NQLLGGIISYAREVKELGLESKFKGKNFVFDERLGERISEEVISHCHQCGKKSDRHVN
                     CKNDACHLLFIQCEECSKEYEGCCSIECRDVSRLPEEEQIRLRKEKAKSGELLEHKSR
                     LRPRLKIHK"
     gene            complement(5107..5979)
                     /locus_tag="PLF66_25120"
     CDS             complement(5107..5979)
                     /locus_tag="PLF66_25120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67394.1"
                     /translation="MNKLIKYLFLSITIAMTLTLFAQDSKTPANNAPAKEGAKSEAKD
                     VTLAMLKDYVAVPALDPSFTVNDISYLRRSAHNGKGDVLDVQINISNNEPSLRDYSIY
                     VLAYHESKIPVTNPLAPPPVWRENDPNKLDTRIDFSVLAPEKITPEDVWGKDGLKARQ
                     DKAEVKKSKGYTFVVGEPSLNEYLLYLSKNTGKALKFKLYYEEVPKEKLTESNYKKSD
                     EELKRDMNNTAATSHTYTVFNSKYKTTITTHHYSEHRANFFFFNRVAVLIFDAKNALV
                     YRSIQEIGKKREHR"
     gene            complement(6080..6436)
                     /locus_tag="PLF66_25125"
     CDS             complement(6080..6436)
                     /locus_tag="PLF66_25125"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020770793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HMZ67395.1"
                     /translation="MLITVLKSKIHRATITDANLNYIGSLTVDKDLMDAAGMIEYQQV
                     AVVNINNGARLETYLIDGKRGSGEICLNGAAARLGEPGDKIIIMTYGLVEEKDHPKDY
                     EPLVVHVDSKNQIIQP"
BASE COUNT         1891 a         1082 c         1119 g         2356 t
ORIGIN      
        1 cgtttctaaa cgccagttga gttcttttac tgttttcata gtttaataaa ttttcatgcg
       61 ttagttctat gttttttctt ttaggcataa cttgaacata taaataacct ttgctgctat
      121 agagttcatt tatagtttgc ttgtctctct gaaattttcc ttcatcaaaa acatcaccta
      181 tgtcatcatt ggagaattca aatagctttt ctaattcttt gatcgaatag attggcttta
      241 agtcttcttt tttcgttcct tctggattgt tttctttatt taaaaagatc ggattccctc
      301 tttcatctag ggtgtaatca tgttttgcct cataaccatt gaaataataa atatcgcctt
      361 ctttaatttt ataattaaca atgattaccc gcttatcttt taattttgca ttttcccatc
      421 tgatctccca attggttccc tctgggacaa tgtctgcatc taagtaacct tttgatttta
      481 aataggctac tatcttttgt ttgtcagatt caaaagaagc ttctttaaaa tgcccactct
      541 cgattagacc cgcttctttt aaatctagaa agcctacgat ttctcccgga tcaattgcct
      601 tcgctccaat aatatttatc ttagagacag gaatttcgtc tccttcatca atgataaact
      661 ttaccttaac taaatttgtt ttaggattaa tcttacccat ttcaaatttg acataggcat
      721 gaaagaaacc ttcgtctcta tacttcttta aaattacttc tcttgttgtg tttactttct
      781 cgggagtaat cacctcattt tctttgatag gcattttatc ccttaagtca gatgggaata
      841 cctcatctgc tccaacgaat tcaatttcct cgactcgggg tctttcttta atttcaaaaa
      901 ttacgattac tcccccgttt gctgctgtat cagctttaat atctatatta tagaagtaac
      961 ccgttgcaaa taacgctttt aaatcatgat ctaataattt ttcggtgaga accatgcctt
     1021 ttcgcatttc gataaagtta agaatatcat ctttgcttgt attttggttt ccaataaatt
     1081 tgatctcgga aattactttt cctagatgta tcgaacgttc agagtatagg ttattgtata
     1141 tctgccccca aaaaataatt agaagcaaaa taaaaatagt tagaaattta gagaggattc
     1201 gaatattcaa aggcaagtat tttactactg atcttcagct ggttcggaat ttcctgaatt
     1261 ttttccctta accatcgcga ggttaaatct tgttaccccc gcagcattaa tctcatcaat
     1321 tacttttacg acagtttgat agtttgctcc actatcgcca cggatgataa ctctattttt
     1381 tttaggatct ctatcttttt tgggaccaag aaaagaaatg attttatctt ttaattgttc
     1441 gagaggaaca ggagttggat ctttatcaag tgtaatcttt cccgctttat caactgtgat
     1501 aataagctca tcctgtgctt tctcttttgt tacactgctt gaagatcttg gtagttctac
     1561 ttttatagac gttccttttt ctaaagtcgc gttaattaaa aaataaatca cgatgaagga
     1621 tactacatct atcagggatg taatatcaat tcgatcgaat gttttcatgg ttcttctaaa
     1681 tttcatactt tctctttatg gaatacttgt aaaaattgat ctgatagatt ttccatttct
     1741 gcaatttttt cttctttttt acgaactaaa taattataga aaatataagc aggaatagaa
     1801 acagaaagac ccattgcagt ggtaatcaat gcttcactga ttccaacctc tgcccctttt
     1861 gttccggcac cttctgcaaa agatcgaatc ataccaacaa ctgttccaag aactccaagg
     1921 agtggaccaa ttgtagctat ggttccaagt ccagttaggt atttttccat tttaaaaatc
     1981 tgtctatacc cttctgattt catttcgtct tcaaaatatt tagactctct tcttttgtat
     2041 tcgatacctg tgacaaggat tgccgttgtt ggaccagtgc tacagctaac taaatagtct
     2101 ttggcttcat cccatttttt ttcaatgaga agatctttta cttttctaaa atcttctggg
     2161 ttaattggtc ttagccgcca aaaatataca gttctttcaa taataatcgt gaaagcgatt
     2221 atggagacaa tgatgattat gatcggaact gtctccggtg gaatagatgc tatgattgag
     2281 tttgatttac ctaaatggaa catttgtaac tgctcttatt tgtgatttaa aagtaaagta
     2341 ttcagagctt gttttactgc caaacacttt attgattttt tagcttctca taaatttcat
     2401 tcgctaggtc tggtctatct gtgattagac cgtcgactcc gagtttgatt aatttttcca
     2461 tttttactgg atcattgaca gtccaaggaa aaattttgta tcctaagcca tggtacttct
     2521 ctatataatc ggaatttacc atatcttctc ttggagcaag tatatctggt tctgccttcc
     2581 catttaaaag tagatttctt aatacaactt tcacaatcca gctcaattct ttgacatcat
     2641 caaagttaga atagagttgt cctcttagaa tttctggatt ggtatgtttg atggtttcta
     2701 gaacataggg gttaaagctt gttacaaaca cttgtttctc taatttctta cttccaacta
     2761 attttgccac cgcagccccg attctttgag cttcctcttt tggtgcttgt gctttgattt
     2821 caatgtctac caaggttttt ccaccaaact catttaaaac ttcttccaag gtcggaattt
     2881 tttctccttt gaactttgga tttgagagtt tggatgcatc tagttgtttt agttctgtca
     2941 atgtaagaga gtctaccatt ccctttccat ttgtggttct atctacatca gaatcatgaa
     3001 tgactactaa ttctccagac tttgcaaaca ttgtatctaa ttcaaaggca gatttatttt
     3061 ttgctgcaaa tctaaatgag gagagtgtat tttctggtgc aattgagtta tcaccagttc
     3121 ctctatgtcc aatcactaaa aacttttgtg gaatcacttc ttccactttt ttagttcttg
     3181 gattatgaga agcgcaagcg gtatagctta cgaaaagaat tggaatcagt agggagtgaa
     3241 agtatttcat aaaaacctcg ttctaaacgt ttatttcttt aaatcaatct atctaatatt
     3301 tgtctttcct aatttctttt agatcaaatt attgtagtta gtttctaaca agaatttgag
     3361 ttgtataaaa tgaaaaaaga ttatttactt atcatcggtg gcttggtccc tcttttattt
     3421 tccctatttg ttttgaatta tttcttaaaa gatgatcgta aagatttatt tgagtcagca
     3481 aaactattag attcgaaatc ttacccaaat ataaaagaag gtgattttgt taagttttct
     3541 ggaatcctgt cgcagaagaa ttcttttgtg aaagataaat atgttttggc agtaaaagaa
     3601 aaatttgtaa aaggtcaaaa tggaaaacga tctgattggt tcagagaaga gttttatttt
     3661 cagacattat ttgtagaatc atctggagtt cctacatttg aaatcaatgt ggctaatgat
     3721 tatttgcctt gtggaaatga ggttcagatt tcggattcaa ttcctaagaa aagtagggtg
     3781 cttggaatca tttccggtgt gaatgtatcg gctgttggta gggtgacttc tattaatcct
     3841 cttagattag atacagggca tagtctttgt acgggaagta ttgaggacta tgatagttat
     3901 cttaggaaaa aaaacctggg ctatttattt attttaatct gtattgctct tcctagcttg
     3961 ggactcattt attttggatt ctttaaggaa agaaaaatta aaccagagtg attgggatta
     4021 tatgaaagaa gaaatcataa gcaaagacga attaagaaaa cgactacagg aagagaactt
     4081 taagagagtc actctctctt tttatcgcta tacaaaaatt gaaaatccaa atgaattgag
     4141 attacaactt ctttccgaat ggggaaatct aaatgttttt ggacgaatct atctggcaaa
     4201 ggaaggagtg aatgcacagc tttctgttcc cgaatttaac tttgaaaaat tcaaagctca
     4261 gcttaattct ctagaatatt ccaagaatat gcctttaaaa atagcaatcg aagatgatgg
     4321 aaaatctttt tttaaactca ctataaaagt aaaagagaag attgtcgccg atggtttgga
     4381 tgattctagc ttcgatcctt ctaatgtagg gaagcattta acggcacaag agtttaataa
     4441 agcattagaa gatccaaata cagttgtagt cgatgtgaga aatcattatg aaagtgagat
     4501 cgggcatttt gaaaaagcga tttgccctga tgcggataca ttcagagaag aacttcctat
     4561 gattgttgat ttgcttcaag ataaaaaaga caagaaaata ttactctatt gcacaggcgg
     4621 aattcgttgt gagaaagcga gtgcttatct gaagcacaaa gggtttttag atgtaaatca
     4681 actccttggt ggaattatat cctatgctag agaagtaaaa gaattgggtc ttgagtctaa
     4741 gttcaaaggt aaaaattttg tatttgatga aagattaggc gagagaatta gcgaagaagt
     4801 gattagtcat tgccatcaat gcggaaaaaa atcagaccgt catgtgaatt gtaaaaatga
     4861 tgcgtgtcat ttactgttta tacaatgtga agaatgttct aaagaatatg aaggttgttg
     4921 ttctatagag tgtagagacg ttagtcgact tccagaggaa gagcagattc gcttacgaaa
     4981 agaaaaggca aaatcaggag agctattaga gcacaaatct aggctaagac cgcgcttaaa
     5041 gattcataaa taaaaaagcc cgataaatcg ggctttccta ctaagatagt atcttatata
     5101 taaagattat ctgtgttctc ttttctttcc aatctcttga atgcttctat aaacaagtgc
     5161 attcttcgca tcgaaaataa gaactgcaac acgattgaag aaaaagaaat ttgctctgtg
     5221 ctcagaatag tggtgagttg taattgttgt tttatacttt gaattgaaca cagtgtatgt
     5281 atgagaagta gcggctgtgt tattcatatc tctttttaat tcttcgtctg actttttgta
     5341 gtttgattca gttaattttt cttttggaac ttcttcatag tagagtttga attttagagc
     5401 tttaccagta tttttagaaa gataaagtaa gtattcattt aagctaggtt ctccaactac
     5461 aaatgtataa cctttgcttt ttttaacttc agctttgtct tgtcttgctt tgagtccgtc
     5521 ttttccccaa acatcttctg gagttatttt ctcaggtgct aaaacggaga aatcaattcg
     5581 tgtatctaat ttgtttggat cgttttctct ccaaactgga ggtggagcga gtggattggt
     5641 tacaggaatt tttgattcat gataagctaa aacataaatt gagtaatctc tgagagatgg
     5701 ttcattgtta gagatattga tttgaacatc taaaacatct ccttttccgt tgtgtgctga
     5761 tcggcgaaga taagatatat catttactgt aaaagatgga tctaatgcag gcactgctac
     5821 atagtcctta agcattgcaa gtgtaacatc ttttgcttct gattttgctc cttcttttgc
     5881 aggcgcattg ttcgctggag ttttactgtc ctgagcgaat aaagtgagag tcattgcaat
     5941 cgttatagat aaaaaaagat acttaattag tttattcata gatttgattc atccgttggt
     6001 gtaatattca ttattatcgt atttttttag gaatataatt agatgtaaag gcaaatattg
     6061 aaatctattt agaaagtttt tatggttgga tgatttggtt tttgctgtct acgtgaacta
     6121 ctaatggttc atagtcctta ggatggtctt tttcctccac aagtccgtat gtcatgatga
     6181 taattttatc gcctggttca ccaagtcttg cggctgcccc atttaaacag atttctcccg
     6241 agcctctttt cccatcgatt agataggttt caagtcttgc tccattgttt atatttacaa
     6301 cggccacttg ctggtattca atcattccag ccgcatccat aaggtctttg tctactgtca
     6361 gactcccaat gtaatttaaa ttggcatctg tgattgtggc tcggtggatt ttacttttta
     6421 gaactgtgat tagcattagg attcgaat
//
LOCUS       DAOQOS010000211         6418 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_6950_length_6418_cov_6.666195, whole genome shotgun sequence.
ACCESSION   DAOQOS010000211 DAOQOS010000000
VERSION     DAOQOS010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 6418)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6418)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6418
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6950_length_6418_cov_6.666195"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..309
                     /locus_tag="PLF66_25130"
     CDS             <1..309
                     /locus_tag="PLF66_25130"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HMZ67396.1"
                     /translation="FYSGIGRRIAEARQSLGISQEDAAKEINVTRVTWNHIEKGNQKL
                     SLDRLLDIAKLLQISPSKLVPGFVANDPTDVVTEKNFSSDEQNLVLEKLAKYRKDKKK
                     "
     gene            336..854
                     /locus_tag="PLF66_25135"
     CDS             336..854
                     /locus_tag="PLF66_25135"
                     /inference="COORDINATES: protein motif:HMM:NF017885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HMZ67397.1"
                     /translation="MESKFKEEILKLYALINYKSGFVDVNKIASKLGMQVIEEDLPDN
                     VSGVLDCRTKEEPIVLLNKHHHLNRKRFSLAHEIGHLVLHKMTGVHMDTKIFLRSDSP
                     SLQGVKIEREANQFAAELLMPELEIRNAWKNLPEDWFDESPLQIIAKQFKVSEGALFI
                     RMKQLKGIGLIW"
     gene            complement(976..2400)
                     /locus_tag="PLF66_25140"
     CDS             complement(976..2400)
                     /locus_tag="PLF66_25140"
                     /inference="COORDINATES: protein motif:HMM:NF012593.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HMZ67398.1"
                     /translation="MKIKLSLQIKILLGTLLVTTLGAVLFGSFFLNTLKAIAIPLAFF
                     IIKSFIRRKTNLQTEILVSMLFGVIFGSIAIHYRLVEFTAMYVKPYGSIFISLLKAIA
                     VPLVFVSLVKGISSLSDMSRLSRLSIRTIILYMSTTVIAISFGLLLVNIVNPGNTFSQ
                     EKKQELREKFKKEAESKAEDAKKVNDHPPLQFLLDFIPDNMIKAAGDNSKMLQIIFIA
                     IIFGIAMIMLPDKDVLGFKNFIDSLNEIILKVIDIIMLYAPIGVFALISSLLVEFGGN
                     NLSDALDLFLALGSYSLTVIVGLMLMLFFIYPSLIFIFTKVKPSHYLKAVFPIQMVAF
                     STSSSAATLPVTMENCEKNLGISKEVSSFVLPIGATINMDGTGLYQSVSAVFIAQVFG
                     YELNLSQQLNIILTATLASIGTAGVPGAGIVMLVIVLNAIGIATEGIALIFAVERILD
                     MFRTVVNVTGDAAVAVIVDSKDRV"
     gene            complement(2418..2846)
                     /locus_tag="PLF66_25145"
     CDS             complement(2418..2846)
                     /locus_tag="PLF66_25145"
                     /inference="COORDINATES: protein motif:HMM:NF024861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HMZ67399.1"
                     /translation="MKDRVFLDSDVILDIAIARKPFLLAAANLLSQIEAGIFDGYTSS
                     IIFTNIYYIQKKLTSHEVAVSFLKKLRLILKVLPVDDMIIQKALESGMKDFEDSVQYF
                     ISTKHKIDYIITRNVEDFKNSIINVHTPEEFLILKGIKEN"
     gene            complement(2843..3106)
                     /locus_tag="PLF66_25150"
     CDS             complement(2843..3106)
                     /locus_tag="PLF66_25150"
                     /inference="COORDINATES: protein motif:HMM:NF039417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6364 family protein"
                     /protein_id="HMZ67400.1"
                     /translation="MNTKLTLNIEEDIIEKAKEYAKSKNRSVSKLVEEYLRSLAFAKT
                     QKKDKPLSLEPITKELVGMIKVKNPEKINYKDILTKSLIEKYK"
     gene            complement(3381..4064)
                     /locus_tag="PLF66_25155"
     CDS             complement(3381..4064)
                     /locus_tag="PLF66_25155"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HMZ67401.1"
                     /translation="MIDSQKEIELVNASGSGDLLRVRTLIEKGADVDFKNSDGYTALM
                     RASIFGNIEVLQYLLGSGGNTEIKDHNGFTALMLAANKGHIEIVKALVKAKAVINAVN
                     KDGWSVLMLASYKGHYEVVKFLLENKAEVNAKDKAGWTPLMFASSNGNIEVVKILLKY
                     KADVSIRNKQGLTALMRAASFGHLEVVKCLVENNSDLSAKNETDYTSLMIAAKNGHKE
                     VVKYLESKT"
     gene            4458..5129
                     /locus_tag="PLF66_25160"
     CDS             4458..5129
                     /locus_tag="PLF66_25160"
                     /inference="COORDINATES: protein motif:HMM:NF024571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HMZ67402.1"
                     /translation="MFRYLTDLIEKDLKSKMVFLGGPGQVGKTTLAISFLKNGNPEHP
                     AYINWDLWLDKEKLIKGEIPKEPILIFDEIHKYPNWRNLVKGFYDKYRLKTKFIITGS
                     ANLDYYHKGGESLMGRFHSYRLHPFSLQEFSSKGNAKDLETLLKFGGFPEPLFAQNEQ
                     EWRCWQRERLKRVIEDDLQSLENIKEISQIELLISMLPNRVGSMLSLNSLREVLNCSH
                     ASNGC"
     gene            5264..5581
                     /locus_tag="PLF66_25165"
     CDS             5264..5581
                     /locus_tag="PLF66_25165"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67403.1"
                     /translation="MVASHLLKYSHYIEDREGYDMELHYCRDREGREVDFIVTKDKKI
                     QFVVDCKLQDKTPSKHLLYFKERLKIPKCYQVHLGKSDFRDEEINIRVLPFWKFSKEM
                     NLV"
     gene            5688..6263
                     /locus_tag="PLF66_25170"
     CDS             5688..6263
                     /locus_tag="PLF66_25170"
                     /inference="COORDINATES: protein motif:HMM:NF017496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HMZ67404.1"
                     /translation="MKNLARQLLTESEYLALERNSETKHEYYKGEMFAMAGAKKKHNQ
                     IVFNIVGQLYIQLKDKPCIAFNSDMRVQVKANGLYTYPDISALCGEEKYLDEKEDTLL
                     NPSLIVEVLSESTETYDRGKKFILYRELESLREYVLVSTEYKKVEIFRRTQNNQWLLT
                     DSLESEPIVFETIQVSLSYEEIYNKVNFPSA"
BASE COUNT         2193 a         1117 c         1097 g         2011 t
ORIGIN      
        1 ttttactccg gcattggcag acgaattgcc gaagcccgtc aatcattagg catttcacag
       61 gaagatgccg caaaagaaat aaatgtcact cgggtcactt ggaatcatat tgagaaagga
      121 aaccagaagt taagtctaga taggcttcta gacattgcca aactcttaca aatttcccca
      181 tcaaaactag ttcccgggtt tgtcgcaaac gatccaactg acgtagtgac agaaaagaat
      241 ttctcttccg acgaacagaa tcttgttcta gagaaattag caaaatatag aaaagataaa
      301 aagaaataag aggaaatctt attcagggga aaccgatgga atcaaaattt aaagaagaga
      361 ttctcaaact ttatgcatta atcaattata aatccgggtt tgtagacgta aataaaattg
      421 catccaaact aggcatgcaa gttattgaag aagatcttcc ggataatgtt tcaggtgtcc
      481 tagactgccg cacaaaagaa gagccgattg tacttttaaa taaacaccac cacttaaatc
      541 gaaaaagatt ttccttagcc cacgaaatcg gtcatcttgt tttgcacaaa atgacaggcg
      601 ttcatatgga taccaaaatt tttcttcgct ccgactctcc atctttacag ggagtcaaaa
      661 ttgagcgcga agcaaatcaa tttgccgcag agcttctcat gcccgaattg gaaatccgaa
      721 atgcatggaa gaatttaccc gaagactggt ttgatgaaag ccctttgcaa atcattgcca
      781 aacaattcaa agtaagcgaa ggtgctctat tcattcgaat gaaacaatta aaaggaatcg
      841 gtctcatctg gtaaaggatt acctacataa caaagaaagc taaaaaacga taagtaatga
      901 agaactatcg tatcgtttat cggtgctcat cggtggtaat ctttttcctt ttctattaag
      961 ttgatctagt ccctactaca ctctatcttt actatcaaca atcacagcga cagccgcatc
     1021 tccagtaaca ttgacaacag tgcggaacat atcgagtatc cgctctactg caaagataag
     1081 agcaatgcct tctgttgcaa ttccaattgc atttaaaaca attactagca ttacaatccc
     1141 tgcaccagga acaccagccg ttccaatcga agcaagagtt gctgtgagta tgatatttaa
     1201 ttgttgagaa agatttaatt catatccaaa gacttgtgcg ataaatacag cagagacaga
     1261 ttgatagagt ccagttccat ccatattaat tgtagcgcct atcggtaaga caaagctgga
     1321 tacttctttt gaaatgccta gattcttttc acaattctcc attgtgacag gaagagtcgc
     1381 agcactagag ctggtagaaa aggcaaccat ttgaatcgga aaaactgctt ttaaataatg
     1441 agagggtttc acttttgtaa aaataaaaat gagagaagga tagataaaaa agagcattag
     1501 cataagtccg acaatcacag tgagtgaata agaacctaag gctaaaaata aatcaagtgc
     1561 atcagagaga ttgtttcctc caaactcaac aagtaaagaa gaaatcaagg cgaatacccc
     1621 aatcggtgca tacagcatga ttatatcgat tactttcaaa ataatttcat ttaaagaatc
     1681 gatgaagttt ttaaaaccaa gcacgtcttt atccggaagc atgatcattg caattccgaa
     1741 aataatagca ataaaaataa tttgtagcat tttagagtta tcgcctgccg ctttaatcat
     1801 attgtctgga atgaagtcga gtagaaattg aagcggtgga tgatcattta cttttttggc
     1861 atcttctgct tttgactcag cttctttttt aaatttctct ctgagttctt gctttttttc
     1921 ctgtgaaaaa gtatttccag ggtttacaat attgactagc aatagaccaa agctgatagc
     1981 gatcactgta gtagacatgt agaggataat tgttcttatg ctaagtctag agagtcttga
     2041 catatcgctt aaactagaaa ttccttttac gagtgataca aacacaagag gaactgcaat
     2101 tgccttcaaa agactaataa aaatagatcc gtatggtttt acatacatcg ccgtaaattc
     2161 aacaagtctg tagtgaattg caatcgaacc aaaaatcact ccgaatagca tactcactaa
     2221 gatttcagtt tgtagatttg tttttcttcg gataaaagac ttaataataa aaaaagcgag
     2281 tgggattgcg attgccttta acgtatttag aaaaaaggat ccgaatagaa ctgctccgag
     2341 tgttgttaca aggagggtgc cgagtaaaat tttaatttga agacttaatt ttattttcat
     2401 gatgactctc taataattta gttttctttg atacccttga gaattaaaaa ctcttctggt
     2461 gtatggacat ttataattga attcttaaaa tcttccacat ttcgtgtgat gatataatct
     2521 attttatgtt ttgttgatat aaaatactgc acactatctt cgaaatcttt cattccagat
     2581 tctaatgcct tctgaataat catatcatct actggtaaaa cttttaaaat taaacgcaat
     2641 ttcttcaaaa agctaactgc aacttcatgt gaggttaatt ttttttgtat gtaataaata
     2701 ttcgtaaaaa ttatcgatga agtatatcca tcaaaaatac cagcttcaat ttgactcagc
     2761 aaattagctg ctgctaaaag aaatggtttt cttgcaattg caatgtctaa aattacatct
     2821 gaatctaaaa atacacgatc tttcatttgt atttttcaat taaagattta gttaaaatat
     2881 ccttataatt aattttttca ggatttttta cttttatcat accaaccaat tcttttgtaa
     2941 tcggttctaa ggaaagaggc ttgtctttct tctgcgtctt cgcaaaagca agagaacgca
     3001 aatattcttc tactaattta gaaacagatc tattttttga tttagcatat tcctttgctt
     3061 tctcaataat atcttcttca atatttaaag ttaactttgt attcatacta gcctcacgta
     3121 acatatttta atattattac gtaatcggca agtaaattat tcaaatgact tgaattcgag
     3181 gaagtggtaa ttgaatggat gctcgataga agatctcgag cctgacctcc tcattggaaa
     3241 tcttgggaga tagtgtaaaa acttaaaaaa aatcgaacca cgaagatcgc gagggttata
     3301 gtttcctagg aaatttacaa tttttcttcg tggccttcat gcacttcgtg gtgaaaatcc
     3361 tgcttttaca tagtctctta ttaagtttta ctttctaggt atttcacaac ttctttgtga
     3421 ccattttttg cggcgatcat gagtgaggta taatctgttt cattttttgc acttaagtct
     3481 gaattgtttt cgactaagca ttttacaact tcaaggtgac caaacgaagc ggctctcata
     3541 agagcagtga gaccttgttt gtttcgaatc gatacatctg ctttgtattt cagaagaatt
     3601 tttactactt ctatatttcc attggaagaa gcaaacatga gtggagtcca acccgccttg
     3661 tcttttgcat tcacctctgc tttattttcg agtaaaaatt tcacaacttc ataatgtcct
     3721 ttataggaag caagcatgag aactgaccag ccatctttat tgactgcatt gatgactgcc
     3781 tttgctttta caagagcttt tactatttca atatgaccct tattggcggc aagcatgagc
     3841 gcagtaaatc cattgtgatc ttttatctct gtatttccac cactacccag taaatattgt
     3901 aatacttcta tatttccaaa gatagaagcc cgcataagcg ctgtatatcc gtctgaattc
     3961 ttaaagtcaa catcggctcc tttttcaata agagtccgaa cgcgaagcaa atcaccactt
     4021 ccagacgcat ttactagttc tatttctttc tgactgtcaa tcatttattt cctcttgcgg
     4081 gcatagtgat atctgatcat tgtaaaatgt aaagaaaaaa aggagaattg cttctctaaa
     4141 ttttttatat accaaatgac aaaagtaatt tcttacgaga actttatcgg agtagcattt
     4201 ggtataccga acatactgat gttcgggaac actaagaagt gtttagacca aacccgcctt
     4261 cttatttttg aaatgagatt tttcttttga ggtttggtat agggaggttc tatacttttg
     4321 attctcgata gaagatttcg atgatgaatt tcttgaataa aaggctattt attagatcat
     4381 gataaatttc cactacaacc aaaatttgtc ataacatttt tccacttgaa cagaataatg
     4441 tcatagaggg attttgaatg tttcgctatc ttactgatct gattgaaaaa gatttaaaaa
     4501 gtaaaatggt ttttttggga ggacctgggc aagttggtaa aacaacatta gccatctctt
     4561 ttttaaaaaa tggaaatccc gaacatcctg cgtatatcaa ctgggatctc tggttggata
     4621 aagaaaaatt aattaaagga gaaattccga aagagcctat tcttattttt gatgaaattc
     4681 ataaatatcc gaattggcgt aatctagtaa aaggatttta cgacaaatat agactgaaaa
     4741 ctaaatttat tataacaggt tccgctaatt tagattacta tcacaaaggc ggcgaatctc
     4801 ttatgggaag atttcattct tatcgtttgc atccattttc acttcaagaa tttagctcaa
     4861 agggaaatgc aaaagattta gaaacacttt taaagtttgg aggatttcca gaaccactct
     4921 ttgcacaaaa tgaacaagaa tggcgatgct ggcagagaga aagactgaaa cgagtgatcg
     4981 aagatgattt gcagtcatta gaaaatataa aagaaattag ccaaatagaa cttctaatca
     5041 gtatgctacc aaatcgagtg ggctctatgc tttctcttaa ttcactacgc gaagtattga
     5101 attgttccca tgcaagtaat ggctgttaat cgcagagcat ctttacctaa cgtttcgaat
     5161 tcctcctttt attcacaaaa atgaaagagc gattaaaaaa gaaaaaaaat tatacttatg
     5221 ggattggttt atctgtgaaa atgaaggaac taggttcgaa aatttggtcg caagccattt
     5281 actgaaatat tctcattata tcgaagacag ggaaggatat gatatggagc ttcactactg
     5341 tagagataga gaaggaagag aagtagattt tatagtaaca aaagataaaa aaatccaatt
     5401 cgttgttgat tgtaaattac aagacaaaac accctctaaa catttactct atttcaaaga
     5461 acgtttaaaa attccgaaat gctaccaagt tcatctaggc aaatctgact tccgtgatga
     5521 ggaaattaat attcgagtat tgcctttctg gaagttttca aaggagatga atctagtgtg
     5581 atagaaatac agattcgggg acgagtttta gaaattacgg ctttttgtta caaaaaccct
     5641 tcttttttat acttgcatta tattgattat tcaaaaaaaa ttgagtcatg aaaaaccttg
     5701 cacgacaatt gttaacagaa tctgagtatc ttgccttgga aagaaattcc gaaacaaagc
     5761 atgagtatta taaaggcgaa atgtttgcaa tggctggtgc aaaaaagaaa cacaatcaaa
     5821 ttgtttttaa cattgtagga caattatata ttcaactcaa agataaacct tgtatagcct
     5881 tcaactcaga tatgagagta caagtaaaag caaatggtct atatacctat ccagacattt
     5941 ccgcattatg cggtgaagaa aaatatctag atgaaaaaga agatactttg ttaaatccta
     6001 gtttgattgt ggaagttctt tctgaatcta ctgagactta tgatagagga aaaaaattta
     6061 ttctctatcg ggaattggaa tctctgagag aatatgtctt agtatccaca gaatataaaa
     6121 aagtagaaat ttttaggaga acacaaaaca atcagtggtt gctaacagac tctttagaaa
     6181 gtgagcccat tgtatttgaa acaatccaag tttccctctc ttatgaggaa atttataaca
     6241 aagtaaattt cccatccgca taaactaaca gcaatttgcc acaataactg tcactctcga
     6301 gatcccctat cgggaatatg tattacgaaa aagtaaccgt tatccgaaag atccctttag
     6361 caaacatatc cttacccaca attttaacag attaccaccg atgacaccga taacagaa
//
LOCUS       DAOQOS010000212         6378 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_7034_length_6378_cov_6.491064, whole genome shotgun sequence.
ACCESSION   DAOQOS010000212 DAOQOS010000000
VERSION     DAOQOS010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 6378)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6378)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6378
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7034_length_6378_cov_6.491064"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            66..986
                     /locus_tag="PLF66_25175"
     CDS             66..986
                     /locus_tag="PLF66_25175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004459221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HMZ67405.1"
                     /translation="MKAIELKNVTKKFKDLTAVNDLSVSIERGEYVALLGPNGAGKTT
                     LVEMIEGIQRPDSGEILILGKNWKENESELKNKLGFSLQETHFIPKLTVEETVKLFAS
                     FYIHQDRTSDILSITGLESKKKTYVENLSGGQRQKLALSIALIHNPEILILDEPTTGL
                     DPTARRDIWNILFELKKKSTTLILTTHYMEEAEYLCDRILIMNQGKFIAQGSLESLLV
                     EYDLGEIIEFSLSGKAKEFQVKKFSGLKEFKWNPETKNGQMIVKDIVTAMPILLDKIK
                     KEKWKLEKFECRKMTLDDLFLSMTGRRLDA"
     gene            979..2019
                     /locus_tag="PLF66_25180"
     CDS             979..2019
                     /locus_tag="PLF66_25180"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HMZ67406.1"
                     /translation="MLNTIYQLVIAEFKEFYRNPGILFWALGFPLIIACILGFAFSRK
                     QDVNRNVGVILQDSNSKLVESLQKKAPDNESYTKFTFIPYSLEEAKLAMKRGKISLYM
                     EKKGNNYKYYFDPNNNEGYLTYLLLDRLLSGIPKSKDQIEKLESKGDRYIDFLIPGLL
                     ALGIMNSCLWGTAWTLMDMRSKKLLRRLISTPLRKPVFLLSHFISRSVLSGLEYFFLM
                     LFAYFLFNVEVQGSFLALFILFIAGTFAFSGIAIFASSRASNSQIANGVINAISFPMM
                     MLSGIFFSYHNFPEWATSIIRYFPLTLLADSIRATFIEGASLNLAIIPSVILVCFGMI
                     TFAVGIRIYKWD"
     gene            2023..3207
                     /locus_tag="PLF66_25185"
     CDS             2023..3207
                     /locus_tag="PLF66_25185"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HMZ67407.1"
                     /translation="MNFVQFYRYATVLLLLLFAFSKLFPQENQTLEPTQNPDKKETTP
                     EKNPHYAALLGIVPGVGQAYIGNYYTGAAQAGTFLSIYNLERYYRSQPDYISYKDREV
                     KFDFNDAVVGYTFQKNGWVYNDLPLKAAVQENDQYKNLSFALFSETRYDRDLRLWKER
                     QLAEENTFIKYGTYSRTSRNTVYADMLNNPVLSTMMYSIYSAYRDAGALGDTKKSETL
                     AQIAYAPFNPDILKKPEVYIPITILGGFFALYSKSGNPILVPSSVKRDGSLYMQAFTD
                     GISPAIGEEAFFRGYLNHTLTTNYGPVAGIGTSSLLFMLAHEGNSDAREGRIARYLAG
                     IYFGWIHYKNGYDIRPGTAVHFWNNFFIGLAAMSQYKADPNYDKGQRDVFFMPIQFTF
                     NL"
     gene            3217..3576
                     /locus_tag="PLF66_25190"
     CDS             3217..3576
                     /locus_tag="PLF66_25190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67408.1"
                     /translation="MKYNLILKILSIFISFFVVNCDLPEQTTESGDNVNLGYAIALKS
                     SECGNLPNYPLYIAGGNAKPTQKDVQACSLAIIQQKCPFTNYPYYCLKIFKNIKIKID
                     NPLSKNDKNKNEMNLDR"
     gene            3577..4203
                     /locus_tag="PLF66_25195"
     CDS             3577..4203
                     /locus_tag="PLF66_25195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67409.1"
                     /translation="MMKRLLAFLFLIFFLYCNSKKENTEEIFQNVFAEGGRKNPKILF
                     RKEVNLDLTPENEYFYVIRNGNEEIVVVMDNTRKNLLFKKIFTLLNIGPLLYDEQKKK
                     WIATSQPMEADALYVVKSLLFMRLGNDSFDSIFIETLSEEPPLDLFSIPMVYRNYQLI
                     FDGFSLFKDSKLLKELNRIPISYDQEKNGIRVLSDKPAFDQFYSLNPK"
     gene            4259..5059
                     /gene="panB"
                     /locus_tag="PLF66_25200"
     CDS             4259..5059
                     /gene="panB"
                     /locus_tag="PLF66_25200"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020776888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HMZ67410.1"
                     /translation="MQTIVNSFKEKFQKKEKISVVTCYDASFARIIANTGMDCILVGD
                     SLGMVFQGNTSTVPVTLEEMIYHTKAVKRGAPDKFIICDLPFLSYQVSVEQGVMAGGR
                     AMKETGCDAVKLEGANDIALETIRRLSSIGVPVMGHIGLTPQSYQVLGGYKVQGKDEA
                     SAQKILQEARQLTDAGAFSIVLEMLPESLGQKITETISVPTIGIGAGRYTSGQVLVIN
                     DLLGVNSDFKPKFLKRFADLNSIVKEALSTYHNEVKSGIFPSEENVFK"
     gene            5308..>6378
                     /locus_tag="PLF66_25205"
     CDS             5308..>6378
                     /locus_tag="PLF66_25205"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HMZ67411.1"
                     /translation="MNPKSPIAAKGVLDLRYVCDTSNNMDESNEQLCLFPTVDLSGEW
                     EFYPNEFFGSTPLTDKNPFPEHYVAPQGGNIEFSRREPFYITVPSAWNDFDFGGKTMG
                     AKGYGTYRLKIFLPNSHFDPEGGKGVSLLQENQKSNLPTLGIKIPTIGSAYQLWVNGK
                     RSAFVGKVSELEESATPGFIPEIILLEPKYIKTQSNGMQVIDLVIQVSNYHIGFGGGL
                     WHALKIGSYSNLSNMNQLTMIFDFFLFGAILIMGLHHFGLYLFRKKDHSTYWFGLFCL
                     LVSTRILLDSGGQCYLERIIPNSFKAVRFLEYLDAILVTPVFVSFLYHVFPDEFLAYV
                     RKGIALVAFSFVFLIILLPTFYY"
BASE COUNT         2063 a         1006 c         1203 g         2106 t
ORIGIN      
        1 tggttaggaa aaccttctag agagattttt agtgatcaat ctcctgtgag cacagagaaa
       61 gaagcatgaa agcaatcgaa ttaaaaaatg tcacaaaaaa attcaaagat cttacagcag
      121 taaatgatct cagtgtatcc attgagcgag gagagtatgt tgcactcctc ggtcctaatg
      181 gagcgggaaa aacaacgtta gtagaaatga ttgaaggtat tcaaagacca gattctggag
      241 aaattttaat tttaggaaag aactggaaag agaatgaatc agaattaaaa aataaattag
      301 gtttctcttt gcaagaaact cattttattc ccaaattaac agtggaagaa accgttaagt
      361 tgtttgcaag tttctatatt catcaggata gaacctctga tattttatct attaccggtc
      421 tagaatctaa aaagaaaacc tatgtagaaa atctttcggg tgggcagaga caaaaattag
      481 ctttgagtat agccctcatt cataacccag agattttaat tttggatgaa cctactacgg
      541 gactagatcc aaccgctagg cgagacatat ggaatatcct atttgaactt aaaaagaaat
      601 caaccactct catattgact actcattaca tggaagaggc tgaatatctt tgtgatagaa
      661 ttctcattat gaatcaaggg aagtttatcg cacaaggaag tctggaaagc cttttagtag
      721 aatatgatct tggagagata attgaattta gcctctctgg aaaggcaaaa gaatttcagg
      781 tgaagaaatt ttccggactc aaggaattta aatggaatcc tgaaactaag aacgggcaga
      841 tgattgtaaa agacattgtg acagcaatgc cgattctctt agataaaatt aaaaaagaaa
      901 aatggaaatt agaaaagttt gaatgtagaa aaatgacgtt agacgattta tttttgtcta
      961 tgacaggaag gagactagat gcttaataca atctatcaac tagtaattgc tgaattcaag
     1021 gagttttatc gaaatcctgg gattcttttt tgggcacttg gttttccatt aatcattgcg
     1081 tgtattttag gatttgcctt tagtcgtaaa caagatgtta atcgaaatgt aggagtgata
     1141 ctgcaagatt ctaattctaa gttggtcgaa tctttgcaaa agaaagctcc tgataatgaa
     1201 agttatacga agtttacttt tattccctat tcattagaag aagcaaagct tgcaatgaaa
     1261 cgtggaaaga tttctctcta tatggaaaaa aaaggaaata attataaata ttattttgat
     1321 ccaaataata acgaaggata tcttacttat ttacttctag atagactttt atctggaatt
     1381 ccaaaatcaa aggatcagat agaaaagctt gaaagtaaag gagatcgtta tatcgatttc
     1441 ttaattcctg gactgttagc tcttgggatt atgaattctt gtctttgggg aacagcttgg
     1501 acattgatgg atatgagaag taaaaagctt ttgagacgtt taatttctac tccacttaga
     1561 aagccagtct ttctactgtc gcattttatt tcccgttctg tgctaagcgg attggaatat
     1621 ttttttctta tgctatttgc gtattttcta tttaacgttg aagtgcaggg aagttttctg
     1681 gcattattta tcttatttat tgctggaact tttgccttta gtggaattgc aatctttgct
     1741 tcctctagag ctagtaactc tcaaattgcg aatggtgtta tcaatgcgat atcatttcca
     1801 atgatgatgc tttctggcat tttcttttcc tatcataatt ttcctgagtg ggcaacgtct
     1861 attatccgtt actttcctct aacactactt gctgattcaa ttcgagcaac ctttatagaa
     1921 ggagccagtc taaatttagc aattattcct tctgtgatcc tagtttgttt tgggatgatc
     1981 acttttgcag ttggaattag aatttataaa tgggattagt aaatgaattt tgttcaattt
     2041 tatagatatg caacggtgct tcttttatta ctgtttgcat tttctaaatt atttccacaa
     2101 gaaaatcaaa ctctagaacc aactcagaat ccagacaaaa aagaaactac tcctgagaaa
     2161 aatcctcact atgcggcttt acttggaatc gttcccggag tagggcaagc ctatattgga
     2221 aattattata caggcgcagc gcaggcagga acttttctaa gtatttacaa tctagaaaga
     2281 tactatcgca gtcaaccgga ctatattagc tataaagata gagaggttaa gtttgatttt
     2341 aatgatgcgg tagtaggata tacatttcaa aagaatggtt gggtttacaa tgacttacct
     2401 ctcaaagctg ctgttcaaga aaatgatcaa tataaaaact taagttttgc actcttttct
     2461 gaaacacgct acgatagaga tttgcgactc tggaaagaac gacagttggc agaagaaaat
     2521 acttttataa agtatggaac ttattctaga accagtcgaa atactgtcta tgcggatatg
     2581 ttaaataatc ccgttttgtc aacgatgatg tattctatat attccgccta tcgagatgca
     2641 ggtgctcttg gggatacaaa aaaatctgaa acactcgctc aaatagcata tgctcctttt
     2701 aatccagata ttttaaaaaa accggaagtc tacattccga ttacaattct tggaggattt
     2761 tttgctcttt attccaaaag tggaaatccg atccttgttc cttcgagcgt taaacgggat
     2821 ggatctttgt atatgcaagc ttttacagat ggaatttctc cggcaatagg agaagaggct
     2881 ttttttagag gctatttaaa tcatacactt acaacaaatt atggtcctgt tgcgggaatc
     2941 ggaacttctt cattattatt tatgttagct catgaaggaa attccgatgc gagagaaggt
     3001 aggattgcga gatacttagc tgggatttat tttggttgga ttcactataa aaatggatac
     3061 gatatacgac ctggaacggc tgtgcatttt tggaataatt tctttattgg acttgctgca
     3121 atgtctcaat ataaagccga tccaaattat gataaagggc agagagatgt tttctttatg
     3181 ccaattcaat ttacatttaa tctctaggta agtaaaatga aatataattt aatactcaaa
     3241 atactatcga tatttatttc cttctttgtg gttaattgtg atttgcctga acaaacaact
     3301 gaatctggag ataatgtgaa tctaggatat gcaattgctc tcaaatcgag cgaatgtgga
     3361 aatcttccta attatcctct ttatattgca ggaggaaatg caaagccaac tcaaaaagat
     3421 gttcaagcct gttctcttgc tattattcag caaaaatgcc ctttcacaaa ttacccctat
     3481 tattgtttga aaatttttaa aaatattaaa attaaaatag ataaccctct cagtaaaaac
     3541 gataaaaata aaaatgaaat gaatttggac agataaatga tgaagagatt acttgctttt
     3601 ttgtttctaa ttttcttttt gtattgcaat tcaaaaaaag aaaacacgga agaaatattt
     3661 caaaatgttt ttgcagaagg aggaagaaaa aatccaaaaa ttctttttag aaaagaagtg
     3721 aatttagatt taactcctga gaatgaatat ttttacgtta ttcgaaatgg aaatgaagaa
     3781 atagtagttg ttatggataa tacaagaaag aatcttcttt ttaaaaagat ttttacactt
     3841 cttaatattg gtccactgtt atatgatgaa caaaagaaga agtggatcgc aacttcccaa
     3901 ccaatggaag cagatgctct gtatgtcgtt aaaagtctcc tctttatgag actcggcaat
     3961 gattcttttg attctatttt tattgaaact ctttccgagg agcctcctct ggatctgttt
     4021 tcaattccta tggtgtatag aaattatcaa ttgatatttg acgggtttag tttgttcaaa
     4081 gattccaaac tattgaaaga attaaatcgc atcccaattt cctatgatca ggagaagaac
     4141 ggtatacgcg tgttatccga taaaccagcg ttcgatcaat tttatagttt gaatccaaaa
     4201 taaattcgag tagacggtat gatttaatga atcattttga gtttagagag ggtagagtat
     4261 gcaaaccatt gtgaattcat ttaaagaaaa atttcaaaaa aaagagaaga tatctgtagt
     4321 cacttgctac gatgcaagtt ttgctagaat cattgcaaat acaggaatgg attgtatact
     4381 cgtaggtgat tccttaggga tggtttttca aggaaataca agcacggttc cagttacatt
     4441 agaagaaatg atctaccaca caaaagctgt aaaaagaggg gctcccgata aatttattat
     4501 ctgcgatctc ccttttttaa gttatcaagt ttctgtggag caaggggtga tggctggtgg
     4561 tcgcgctatg aaagaaacag gctgcgatgc tgtcaaacta gaaggcgcaa atgatattgc
     4621 actagaaaca attcgtcgtc tatcgtctat tggagtgcct gtaatgggtc atataggtct
     4681 tactcctcaa tcctatcaag ttttaggggg atacaaagtc caaggcaagg atgaagcatc
     4741 cgctcaaaaa atattgcaag aagcaaggca gttaacggat gcaggtgcct tttctattgt
     4801 tctagaaatg ctaccagaat ctcttgggca aaaaattact gaaactattt ctgttcctac
     4861 gattgggatt ggagcaggac gttatacaag tgggcaagtc ctagtcatca atgatctact
     4921 aggtgtgaat tctgatttca aaccaaaatt tttaaaacgt tttgccgatt taaattctat
     4981 tgtcaaagaa gcgttatcta cctatcacaa tgaagttaag tccggtatat ttccttcaga
     5041 agaaaatgtc tttaagtgac taacttcctt caggtttgag caatagctct ttagtatata
     5101 caaagacttc ttgatttgct gattcctaat tagtaaaact tgaggcattt aacgtcattg
     5161 ttgcgtataa cgatcaaaaa ttgaataatg actaaataaa tattgcttta taaaacaagt
     5221 aactatattc tagctattgt tttacattta atgaaaccat ttctaagtct cattcttctt
     5281 attttttcgg agtgtcaata tctagatttg aacccaaagt cgcctatagc tgctaaaggt
     5341 gtgctagatt taagatatgt ttgcgataca tccaataata tggatgaaag taatgaacag
     5401 ttatgcttgt ttcctactgt cgatctaagt ggcgaatggg aattttatcc caatgaattt
     5461 ttcggttcga ctccgcttac cgacaaaaat ccgttccctg agcattatgt tgcgccccaa
     5521 ggaggcaaca ttgagtttag ccgaagggaa cccttctaca tcaccgttcc gagtgcctgg
     5581 aatgattttg actttggtgg aaaaactatg ggtgctaagg gatatgggac gtatcggttg
     5641 aagatttttt taccgaattc ccattttgat ccagaaggcg ggaaaggagt ttctctcctt
     5701 caggaaaatc aaaaatcaaa tttacctacc cttggaataa aaataccgac tatcggatct
     5761 gcctatcaat tgtgggtaaa tggaaaacga tctgcgtttg ttggaaaagt ttctgaatta
     5821 gaagaaagtg cgacccctgg atttattcct gaaatcattc ttctagaacc aaagtatatc
     5881 aagacacaat caaatggaat gcaggtaatc gatttagtca ttcaagtatc gaattatcat
     5941 ataggatttg gtggtgggtt atggcatgcc ttaaaaatcg gaagctatag taatctttcc
     6001 aatatgaatc aattgactat gatttttgac ttctttctct ttggtgcgat tcttattatg
     6061 ggattacatc attttggatt atatctattt cgaaaaaagg atcactccac atattggttt
     6121 ggactctttt gtctacttgt atctactcga attctccttg attctggggg gcagtgctat
     6181 ttagaaagaa tcattccaaa tagttttaaa gcagttcgtt ttttagaata cttggatgcg
     6241 attttagtta ctcctgtatt tgtaagtttt ttataccatg tttttcccga tgaattttta
     6301 gcttatgttc gtaagggaat tgctttggtt gcgttttctt ttgtattctt gataatactt
     6361 cttcctacct tttattat
//
LOCUS       DAOQOS010000213         6331 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_7142_length_6331_cov_7.868388, whole genome shotgun sequence.
ACCESSION   DAOQOS010000213 DAOQOS010000000
VERSION     DAOQOS010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 6331)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6331)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6331
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7142_length_6331_cov_7.868388"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..6218)
                     /locus_tag="PLF66_25210"
     CDS             complement(<1..6218)
                     /locus_tag="PLF66_25210"
                     /inference="COORDINATES: protein motif:HMM:NF015497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpvB/TcaC N-terminal domain-containing protein"
                     /protein_id="HMZ67412.1"
                     /translation="MGNLKNKFGFKLYKLLQRVPHIVLLILLSFFFRNDFTPPEPLYQ
                     TIPQTSVDARGNLNQAYPIQLPLGTKDIIPKLSLAYNSASGNGILGMGWILNGIDFIA
                     RDTSKGVEGYDGRYISSQAGTLELSAGSVYYPKEFNFSKFILQADGSWLSIDKAGNKY
                     YFGVSYGVKTTETDPLRNDKIKIWPLERVEDIYGNGYSIAYLTADSKSDGYPYPDKIT
                     YNNGNTVIQFAYATRSTNDTIELYRYASKQRITKRLDSITVANNGSVIETYILGYELG
                     ATSNSRLISINRDGYKPISFSYNDNTIDVNGITSASSAVSSLNYTYMFPERNPCIIGE
                     AACACSAACAPPLNMIAGVICAVNYMAYKNYCRLGIENSIHLFADVTGDGVPELLAVA
                     GSNSNVQIKSSVAGSSLQSDYFNLSGNGMIIPGDINGDGKTDFFIFEDNDKPLKIAKV
                     NPDLLMPVTTLGVNVSKIRNDAGDKATKNFIVDFNADGRSDYIKFNSDNSALNLFLSN
                     GNTLDIPNPQIELAYNDYGSFGQTFMDMDRNGIPDFVRMDGTTKSIKITFYKRNLTFN
                     SESITTFTDLGEPGNQFFADVNGDGFIDFIIYDGYNLQVYLFDGMKFKKTPMTSPIEN
                     VVATKDPGYDSPDIGPPGQGAENGYIDLTGDNNPDKVEYDGRSAKVNVYNPVTKKYDP
                     AIAIGASPFLVDLDKNGGQDEVKYTTRSITDSNNKLHTYITFKVKMYDKDGNLIANTT
                     PPEVDIGNLYPKARQSQMIGARVYDNWRNTKMFVDLNGDGKADFVRFFNGRVYISYFT
                     NVAGNAVYSKSGDISFAAAAFQYVADMNKDGRMDIVGVDAAQSPIQYGFESSDYKNES
                     ARAGSGTLNFYYLPKSSAGDLLTKAYDAQKGISITYGNDQKFDNDSYPVLNHQSTINR
                     IVNSLDIDYAGGNTERRTYFFYDHKYYIGASIDKTKDLGYKIQTILSIKFSPYESKMI
                     YEENLYNQLDAANAPYFPGTLKKNTTYFPNSILQKLVDTETSYTTLAAIGGNSFIAPT
                     SQTITSQDGFTPSITKSTAYDAYGNVTVQSETQGTETNEVVNSYEVDTTNWILNRAKR
                     ATKKINGIVMEDKQVSYDGIYISEMKNLVDPEKQVWSKTTFSNYDGHGNPRTITDSKN
                     NNINLVYDDFANRYVVTATPSMPGHTITKTYNYNNGEEATVTNAFGTLTKEYDRYGRL
                     SIVRYPGVSQNSWSEQYFYNLNDPLNAFVERKINDGSASGLWAREWTNSIGHKYRKET
                     KASVGQNSDTSMVEEKYYDLYGRLTKEAKAYWSSNSPLYKTYEYSDALGRLTKVTHED
                     GSTTDMSYSGFNTTIVTTGKASDGTFKATNTTEVFATQRGKVTKKIVNGLETNYDENF
                     DSKGRYVKITAADGSQTLDRYNLKGDKLSHKDSNSGLTTYEYDPNGNLTKQTGANAGS
                     VIQYEYDPLDRVTKIDKPGFETDTILKYDENNKIGKLTSVVLKEYKQDVGIWKDAVVS
                     TYDYDAIGRLSSLKKEVDDLKLIFRYQYDILNRVNKVTYPNQVSLNYKYATSGYIQSI
                     SMDKGNDLDNEVVAYAVTPEFVLQRLTGNNVNMQITYDAATKRPKDVVTKLNMTDAVQ
                     KWHYDYDHAGNLLKITDIIAGGNSQSYTYDNYNRISSATGLYGLDKKLATENYEYTDG
                     GRLTHKANLDLFYEDAKHLHAATRAKVKTALNTTYDVNYKYDDAGNMVQRDDEIFKYD
                     SENKLVQVDKTVGDTVNYLYEHTGNRIRKSRTSTNEVVYNINGVYEVTKIPSMSDRHT
                     LYIRGIKEELVAQYTATDIVLLTDSNLIEKDKAGLIGLFSYWKNTLDQHRIRFAKWVA
                     RTTYENPFALKLYTVYIIFFFILLFAVIEALEMTTKSNTVSSSSNVSSSDSRELQINE
                     TSYASSSRVPRTDNKKKPFYQVVLDSARTDNKGLRLVSLLLIVSVTFLFSNCSPKALT
                     GGNRPQTPPYWLLGAFANEPASKSVSTGGGNNPASPSSGFGGSASGSLSAGTPRLGMF
                     FFHPDHTGSISMVTDGAGRMMTDTSGASQLTYKPYGEVNRMNSYGPDIFRYKYTSQ"
BASE COUNT         1787 a         1274 c         1090 g         2180 t
ORIGIN      
        1 tgttgactcg tatatttgta tcggaatata tcaggaccat agctattcat acgattcact
       61 tcaccgtatg gtttgtaagt caactggctt gcgcctgacg tgtctgtcat catccttccg
      121 gctccgtctg tcaccattga aatagagcca gtgtgatctg gatgaaaaaa gaacattcca
      181 agccttggag tgcctgcgga taatgagccg ctggcgcttc cgccaaatcc agaagaggga
      241 gatgctggat tatttcctcc acctgtagaa acagacttgg aggctggttc atttgcaaat
      301 gctccaagca accaatacgg tggtgtctgc gggcggttac ctcctgttag tgccttcggt
      361 gaacaattac tgaataggaa tgtaacggaa acgattagaa gtagggatac taggcgtaag
      421 cctttattgt cagttcgagc ggagtcgaga actacttgat agaaaggttt tttcttattg
      481 tcagttcgag gaactcgaga actactcgca taagaagtct catttatttg tagttctcga
      541 ctatcgctcg aactgacatt gctgctcgaa ctgacagtat tactcttagt tgtcatttcg
      601 agagcctcaa tgaccgcaaa aagtaaaata aaaaaaaaga ttatataaac cgtataaagc
      661 tttaaggcaa acggattttc ataggttgtt cttgctaccc attttgcaaa tcgaattcta
      721 tgttgatcta gtgtgttttt ccaataagag aaaagtccaa tgagtcctgc cttatctttt
      781 tctattagat tggagtctgt gagtaataca atgtcagtcg ccgtatattg ggcaactaat
      841 tcttctttaa ttccacgtat ataaagtgta tgccggtctg acatgcttgg aatcttcgtt
      901 acttcgtata ctccgttgat attatagact acttcatttg tagatgttct actcttacgt
      961 attctatttc ctgtatgctc atacagataa ttgacagtat cccctactgt cttatccacc
     1021 tgcactagtt tattttcact atcgtattta aaaatctcat cgtctctttg caccatgttt
     1081 cctgcatcat cgtacttgta atttacatca tacgttgtat ttagggcagt tttaaccttt
     1141 gcgcgagtag ccgcatgtag gtgctttgca tcttcataaa ataaatctag attagcctta
     1201 tgagttaatc tccctccatc ggtgtattcg tagttttccg ttgctaactt tttatctaat
     1261 ccatacaagc cagtggcgga gctaatgcga ttgtaattat cgtaggtata agactgcgaa
     1321 tttccgcctg caatgatgtc tgtgattttt aatagattcc ctgcatggtc ataatcataa
     1381 tgccatttct gcactgcatc tgtcatattt aattttgtaa ctacatcctt cggacgcttt
     1441 gtcgcagcgt cgtatgttat ctgcatattt acattatttc cagtcaaacg ttgtagcaca
     1501 aactcaggcg taaccgcata ggcgactact tcattgtcta agtcatttcc tttgtccatg
     1561 ctgatggact gaatgtatcc agaggttgcg tatttataat ttagggaaac ctgatttggg
     1621 taggtaactt tatttaccct attcaatata tcatactgat atctgaaaat taattttaaa
     1681 tcatctactt ctttctttaa agacgatagg cgaccgattg catcataatc gtatgtagat
     1741 acaactgcat ctttccatat ccctacatcc tgtttatatt ctttgaggac aacacttgtt
     1801 agctttccga ttttattatt ttcatcgtat tttaaaatcg tatctgtttc gaaacccggc
     1861 ttgtctatct ttgtcactcg gtcaagcggg tcatattcat actgaattac acttcccgca
     1921 ttagcccctg tttgtttagt gagatttcca tttggatcat attcataagt agtcaaacca
     1981 gaattagaat ctttgtgaga aagcttatcg ccttttagat tgtatctatc caaggtttgg
     2041 ctgccatcgg cagccgtaat ttttacatat cttcctttgc tatcaaaatt ttcatcgtag
     2101 ttagtttcta aaccatttac aatctttttg gttacttttc ctctctgggt tgcaaatact
     2161 tccgttgtat ttgtagcttt aaatgttccg tctgacgctt ttccagttgt aacgatagtt
     2221 gtattaaagc cggagtaact catatcggtc gtgcttccat cttcgtgagt aacttttgtt
     2281 agcctaccta gagcatcgga gtattcgtat gttttataga gaggcgaatt actagaccag
     2341 taagcttttg cttcttttgt taatcttcca tacaaatcat agtatttttc ttctaccata
     2401 cttgtatctg agttttgtcc gacagatgcc tttgtttctt ttctatactt atgacctata
     2461 gaatttgtcc attctctcgc ccaaagacca ctagcagacc catcattgat tttacgctct
     2521 acaaatgcat tgagaggatc gtttaaatta tagaaatact gttcactcca actgttctgt
     2581 gagactcccg gataacgcac aatagacaat ctaccatacc tgtcatattc ttttgtcaga
     2641 gttccaaatg cattcgtaac agttgcttct tccccattat tataattgta tgtctttgtt
     2701 atagtatgtc cgggcatact gggagtagcc gtaactacat accgatttgc aaagtcatca
     2761 taaactaaat taatattatt atttttacta tctgtaatcg ttcgcgggtt tccatgacca
     2821 tcatagtttg aaaaggtagt ctttgaccaa acttgttttt ctggatcgac caaatttttc
     2881 atttcagaaa tataaattcc atcgtatgat acctgcttgt cttccatgac aatgccattg
     2941 attttcttag tcgctctttt agcacgattc aaaatccaat tcgttgtatc tacttcatac
     3001 gaattgacta cctcatttgt ttctgttccc tgcgtttccg attggacagt cacatttcca
     3061 taagcatcat aggcagtgga tttagtaatc gaaggagtga aaccatcttg gctagttata
     3121 gtctgagaag ttggcgcaat aaagctatta ccccctatag ccgcaagagt agtatagctt
     3181 gtttcagtat ccactaattt ctgtaatatg ctatttggga aataggttgt attctttttt
     3241 aacgttcctg gaaaatacgg tgcgttagcc gcatctaact ggttgtatag gttttcctca
     3301 tagatcattt ttgattcata cggagaaaac ttaatactta ggatagtctg tattttataa
     3361 ccaaggtctt ttgtcttatc aatgcttgcg cctatatagt atttatggtc ataaaagaaa
     3421 taagttctcc gctctgtatt tcctccagcg tagtcaatat ctaaagaatt cacaatccga
     3481 ttgatagtac tttgatggtt taatactgga taagaatcat tatcaaactt ttgatcattc
     3541 ccatatgtaa tcgaaatacc tttttgagca tcataggctt tcgtcagcaa atcccctgcc
     3601 gaactttttg gtaaataata aaaatttaaa gttccgcttc ctgcacgggc agattcattt
     3661 ttataatcag aactttcaaa tccatattgg attggagatt gggcagcatc tacacctaca
     3721 atatccattc ttccatcttt attcatatcc gcaacatatt gaaatgccgc agccgcaaag
     3781 gaaatatctc cactcttcga atacactgca ttacccgcta catttgtaaa ataggaaata
     3841 taaactctcc cattaaaaaa tcgcacaaag tcggcttttc catctccgtt caaatcaaca
     3901 aacattttgg tattgcgcca attatcataa actctagctc caatcatttg tgattgtctt
     3961 gccttcgggt atagatttcc tatgtcaact tctggcggag tagtatttgc tattaagttt
     4021 ccgtctttat cgtacatctt tactttaaat gtaatatagg tatgaagttt gttatttgaa
     4081 tctgttatag aacgcgttgt atacttgact tcgtcttgcc cgccattttt atctaggtca
     4141 acaagaaaag ggcttgctcc tatagcaata gcaggatcat attttttagt tacaggatta
     4201 taaacattaa cctttgcact cctcccatca tattctactt tgtctggatt gttatctcca
     4261 gttaagtcaa tgtaaccgtt ttccgcccct tgtcctggcg gacctatatc cggcgaatca
     4321 taaccggggt ctttggtagc gaccacattc tcaataggac ttgtcattgg agttttttta
     4381 aatttcattc catcaaatag atatacttgt agattgtatc catcgtaaat aataaagtca
     4441 atgaatccat ccccgttcac atctgcgaaa aactgatttc ctggctctcc taaatcggta
     4501 aaagtcgtaa tactttcaga attaaatgtt agatttcttt tgtaaaatgt tattttaata
     4561 gatttcgtag tgccatccat acgcacaaaa tcaggaatac catttctatc catatccatg
     4621 aaagtctgtc caaaggaacc ataatcattg tatgcaagtt caatttgtgg attaggtata
     4681 tcgagtgtat taccattaga taagaataaa ttcaaagctg agttatctga attaaactta
     4741 atataatcac tacgcccgtc agcattgaag tctactatga agtttttagt cgccttatct
     4801 cccgcgtcat ttctgatttt acttacattt accccaagag tagtcacagg catcaacaaa
     4861 tcaggattta cctttgcaat cttaagtggc ttgtcattgt cctcaaaaat aaaaaaatct
     4921 gtctttccat caccattaat atcccctgga ataatcattc catttccaga aagattaaag
     4981 taatcgcttt gtagggaaga gcctgctact gaagatttaa tttgcacatt actattcgaa
     5041 ccagctacag ctagtaattc aggaactcca tctcctgtta catcggcaaa caagtgaata
     5101 gaattttcaa ttcctaacct acaataattt ttgtaagcca tataattgac tgcacatatc
     5161 actcctgcaa tcatatttaa gggaggagcg catgcagcac tgcatgcaca ggctgcttca
     5221 cctatgatac aaggatttct ctcgggaaac atatatgtat agttcaatga gcttactgca
     5281 cttgaagcgg aggtaatacc atttacatct attgtattat cattatacga aaaagaaatt
     5341 ggtttatacc catcgcggtt tatgctgatt agccgactat tagaagttgc gccgagttcg
     5401 taccctagta tataagtttc gatgacagag ccattattag caacagtaat agaatctaat
     5461 ctttttgtaa tcctttgttt cgaagcatag cgatatagct caattgtatc attcgtagag
     5521 cgtgttgcat acgcaaactg aatgacggta tttccattat tgtatgtaat tttatcagga
     5581 tagggataac catcagattt actatctgcg gttaagtatg cgatgctgta tccattccca
     5641 taaatatctt ctactctctc taaaggccag attttaattt tatcgtttcg aagaggatca
     5701 gtttcagtcg tctttactcc atagctaaca ccgaagtaat atttgtttcc cgctttatca
     5761 atggaaagcc aactcccatc tgcctgcaaa ataaatttac tgaaattaaa ttcttttgga
     5821 taatatacac tacccgcact aagttctaat gtgcctgctt ggctggatat ataacgacca
     5881 tcataacctt ctacaccttt tgaagtatcg cgagcaataa aatcaattcc atttagaatc
     5941 catcccattc caagaattcc attgccggaa gctgaattat aagcaagcga taactttggg
     6001 ataatgtctt ttgtccctag cggaagctgg attgggtatg cttggttaag atttcctctt
     6061 gcatcaacag atgtctgagg aatagtttgg taaagaggct ctggcggagt aaagtcgttt
     6121 cgaaagaaaa aagataataa aattaataga acaatatgag gaactctttg caagagttta
     6181 tacaacttaa aaccaaactt atttttcaaa ttacccataa gaatccacct cttctaatat
     6241 tttttcaatt catacacgag caaaacaaaa ctccaatagg tgttaggtgt taggtgttag
     6301 gtgttaggtg ttaggtgtta ggtgttaggt g
//
LOCUS       DAOQOS010000214         6049 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_7695_length_6049_cov_6.100934, whole genome shotgun sequence.
ACCESSION   DAOQOS010000214 DAOQOS010000000
VERSION     DAOQOS010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 6049)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6049)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6049
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7695_length_6049_cov_6.100934"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(300..611)
                     /locus_tag="PLF66_25215"
     CDS             complement(300..611)
                     /locus_tag="PLF66_25215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67413.1"
                     /translation="MNRNGRDLKEAVLNFVSESKSLAIFVPYIKINALKEILSLTKNC
                     RYIVVRWEVKDLLERASDLEIYELCKEKKISLFRNPRLHLKLYLDADKTYLPLSITKK
                     Y"
     gene            complement(616..999)
                     /locus_tag="PLF66_25220"
     CDS             complement(616..999)
                     /locus_tag="PLF66_25220"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HMZ67414.1"
                     /translation="MRIADANTILRFFLKDNSEQFAEAEKVFDSKEEIFIPDEIIAEV
                     VYVLQKVYSVSREEIRARLIEFISYQNVILNESDITINALNAYATSNLDYADCIVYSH
                     AKSKSSDIFSFDKGLLSFFKKNKKD"
     gene            complement(986..1213)
                     /locus_tag="PLF66_25225"
     CDS             complement(986..1213)
                     /locus_tag="PLF66_25225"
                     /inference="COORDINATES: protein motif:HMM:NF021558.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2281 domain-containing protein"
                     /protein_id="HMZ67415.1"
                     /translation="MQLIEERIKSLTPELQKEVIDFIDFLVSKYSLSPDKNSPKTKSV
                     GGSLEKYKNSKLQSKEKGALAEAFAEKHENR"
     gene            complement(1457..1972)
                     /locus_tag="PLF66_25230"
     CDS             complement(1457..1972)
                     /locus_tag="PLF66_25230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67416.1"
                     /translation="MALDHYIQGFSIHTAKDKPIEDEEWDALDKPLVAYSNLLLAVGF
                     YLNKYIDPNYNIPTKVSEVKKIANELESLKDERKKETKEIRIAVEENFLANTRQLFDL
                     PEEYSENNRKKPQNIQKLKKLNQAGIQPIKILHLHVFDYESMGNYRHVVLEAPLSAFA
                     ICWNGKEFLSE"
     gene            complement(2138..3085)
                     /locus_tag="PLF66_25235"
     CDS             complement(2138..3085)
                     /locus_tag="PLF66_25235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67417.1"
                     /translation="MYFNGIDLKKQILEYVKTTDSVAIYVPYIKEKELREILGQTKNC
                     RYVVVRWEPKDILERASDLEIYYVCKERNIPLYRNSRLHLKLYLGEDNALLTTANISA
                     RAMNDGKNDNYNYELGTKIDKLDFEDRLYLQTIVDKSLLLSDEIIEEIKEQLAKLPIN
                     EEGIDFEFKNKTENKDFLLSSLPMTESIEKLRKFYQWKDAPTKEEYECLIHDLSLYEI
                     PAGLAEYDFINELKNKFFSHPFVKAFIDEIRSSGGELFFGRVRQWLAANCANSPTPRA
                     WEVTKNVQILYHWLSELSNGKFRVDVPGTHSQRIRMDLS"
     gene            complement(3091..4899)
                     /locus_tag="PLF66_25240"
     CDS             complement(3091..4899)
                     /locus_tag="PLF66_25240"
                     /inference="COORDINATES: protein motif:HMM:NF024977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HMZ67418.1"
                     /translation="MADRHDIVPPHLAIQAMRDNGYKNTAYAIAELIDNSLQAGAKNV
                     QLLCGEEEIDGARNNRITRISQIAILDDGHGMNEDVLRLSLQFGNGTHLDAKNQQGIG
                     KFGMGLPASSISQCTLVEVWSWQDGVDNALYTFLDIHQIKNKQLIEIPKPKKHKIPKI
                     YSRIGKEFGKSGTLVVWSNLDRILWRKADAIINNSEFLIGRLYRKFLDKKKCSIRMVA
                     FNLHDLINIRIDRYALPNDPMYLMEKTSCPAPFDKTPMFEDYGDKELKLPVNYRGKKY
                     PVTIRFSVAKKEARKGEGAGSREHGKHAARNIGVSIVRAHREIELDKSWTIQYDPMER
                     WWGIELEFPSELDELFGLTNNKQYATNFSHLAEIDYDEIAKGKQSIIEYQEQLEAEED
                     PIAPILDLGNRIKKNLHAMRAHIKAQNAGAHSTRGERQTEDSPEVIATKVTRELIEEG
                     YSSKNDDFEKLPVKERIAEIEKVLVDQGLDKEEAKEISKTATDYGLKYVFAHADIQTP
                     AFFTVRPKAGTIIITLNTEHPAYDNLMEILNEDTTNVSREDLVDRLSRARDGLKLLLE
                     AWARYEDTLPDGNIKDKAQEVRNDWGRMAKRFMTKE"
     gene            complement(4932..>6049)
                     /locus_tag="PLF66_25245"
     CDS             complement(4932..>6049)
                     /locus_tag="PLF66_25245"
                     /inference="COORDINATES: protein motif:HMM:NF037510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HMZ67419.1"
                     /translation="TGSGKTRTTMNYIADYLRKNEGALVIWLAYSEELCEQAVSEFKK
                     AWGYLGNRNINIQRYYSNIQWQDGISEGLVVAGLSKLYNTIRNGSIQFVNDLSDKISL
                     IVMDEAHQAIAESYNTILQILSRKENVSIIGLTATPGRSYLNIDEDEKLANFFHRNKI
                     TLSIEGYSNPIDYLTDNGYLAKVTFEPLTHKGGYTITQEDIAKIERELDLNEEILIRL
                     GQDELRNTAILSRLKSLILYHKRIIFFAASVSHSALIAFMLRAIGIESYSVTGATPDS
                     ERYKAIQEYKSDKEASIVLCNYGVFTTGFDAPKTSCAMIARPTKSLVLYSQMVGRAIR
                     GSKAGGNDNALIVTVVDTKLPGFRSMAEAFMNWEDVF"
BASE COUNT         1749 a         1189 c          867 g         2244 t
ORIGIN      
        1 tgtttgtatt tgtattactg tattatgata aatccatcct tatcctctgc gaatgtgttc
       61 ccggcacgtc tactttgtat ttgccgttgc cgagaaattc gaaccagtgg tagaggattt
      121 gcacgttctt ggttacttcc caagctctag gcgttggaga attcgcacaa ttagccgcta
      181 tccattgtca tcttactttt ttcttttctt ttatggaaag agaaaagaaa aaagtaagca
      241 aaaaagaaaa gaaaacccgc cacagcgact cattgtttta acacgctgta ggactccggt
      301 taatactttt tcgtgattga tagcggtaaa tatgtcttat ccgcatctag ataaagcttc
      361 aagtgtagcc tcggatttct aaataatgaa atcttttttt ctttgcaaag ctcataaatt
      421 tctaaatcac tcgctctttc aagtaaatcc tttacctccc atctcaccac aatgtagcgg
      481 caattctttg ttaggctcaa tatttctttt aacgcattaa tctttatgta tggaacaaaa
      541 atcgctagcg attttgattc gcttacgaag tttaagactg cttcttttaa atcacgcccg
      601 ttgcggttca tgtggttagt cttttttatt tttcttaaag aaacttaaaa gacctttatc
      661 aaaagaaaag atatcactgc tttttgactt tgcatgagaa tatacaatac agtctgcata
      721 atctagatta gaagttgcat aagcattcaa tgcatttatt gtaatatctg attcattgag
      781 aattacattc tgataggaaa taaattcaat cagtcttgct ctaatttctt ctctggaaac
      841 tgaataaact ttctgtaaaa catagaccac ctctgcaatg atttcgtctg gaataaatat
      901 ctcttccttt gaatcaaata ctttttcggc ctcagcaaat tgttctgaat tatcctttag
      961 aaaaaaccgt aggatagtat tggcatcagc gattctcatg tttttctgcg aatgcctccg
     1021 ctaaagctcc tttttccttt gattgtaatt tactgttttt atacttctca agagaaccac
     1081 caaccgattt tgtctttggg gaatttttgt ctggcgataa tgaatattta ctcactagaa
     1141 aatctatgaa gtcaatcacc tctttttgta attcaggtgt tagtgattta attctctctt
     1201 ctattagttg cataattccc tcgttttgaa tctacaatcc attaacattc ttattaaaaa
     1261 taagattcac tcattcaaca tattagcaag caaaaaatct atctttatat agttcctagg
     1321 atgtcgtcaa cttaagaact aagtgtgatg cgattagtag gagaacaggc atggattcga
     1381 taaactcacc acaagtccct gcctgtttct aggacaagta gggattgcta tcataaaact
     1441 aaatcattca taataatcat tcactcaaaa attccttccc attccaacaa atagcaaatg
     1501 cagaaagggg agcctctaga acaacatgtc tataatttcc catcgattca taatcaaaaa
     1561 catgaagatg taaaattttt atgggttgaa ttccagcttg gtttaacttt tttaattttt
     1621 gaatattttg aggttttttt ctattattct ctgaatattc ttccggcaaa tcaaataact
     1681 gacgtgtatt cgccaagaag ttttcttcca ctgctatcct aatttctttt gtttcttttt
     1741 ttctctcgtc ctttagtgat tctaattcat tcgctatttt ttttacttca ctcactttcg
     1801 taggaatatt ataatttgga tctatatatt tattcaaata gaaaccaact gctaggagta
     1861 aattgctgta agccactaga ggcttatcca atgcatccca ttcttcatct tcaattggtt
     1921 tgtcttttgc tgtgtgtatg gaaaatcctt ggatgtaatg atctaatgcc atgtttagaa
     1981 attgttctct ttcgaatttc gtttgtattt cttttttctt ttcttcaaaa ctcgttgcct
     2041 cccattcttt cgataaggga tcaaataggg tttttaatgt tgttaggtat tttcgttttt
     2101 cattggggtt gcctttgttt gtatttgtat tactgtatta tgataaatcc atccttatcc
     2161 tctgcgaatg agtccccggc acgtctactc taaatttacc attacttaat tctgaaagcc
     2221 aatggtagag aatttgaacg ttttttgtta cttcccatgc tctcggtgtt ggagaattcg
     2281 cacaattagc cgctagccat tgacggactc ttccgaaaaa taattcacca cctgagcttc
     2341 gtatttcatc aataaaagct tttacaaatg gatgggaaaa aaacttattc ttgagttcat
     2401 ttataaaatc atattcagca agtcctgctg ggatttcata caaacttaaa tcatgaatca
     2461 aacattcata ctcttctttc gtaggagcat ctttccattg ataaaatttt cttagttttt
     2521 caatgctttc tgtcattgga agagaagata ataaaaagtc tttgttttct gtcttatttt
     2581 taaactcaaa atctattccc tcttcattta taggaagctt tgccaattgt tcttttattt
     2641 cttctataat ctcatcactg agtaaaagac ttttatcaac aattgtctgt aaatatagtc
     2701 gatcctcaaa atctaattta tctattttgg ttccaagctc ataattataa ttatcatttt
     2761 tcccatcatt cattgctctt gctgaaatat tagctgtagt taataatgcg ttatcctctc
     2821 ccaaatataa ctttaaatga agtctagaat ttctatagag aggaatgttt cgttccttac
     2881 atacgtaata gatttctaaa tcacttgctc gttctaatat atctttgggt tcccaccgaa
     2941 ctacaacata gcggcaattc tttgtttgcc ccaatatctc tctaagctct ttttccttta
     3001 tatacggaac atagatcgca actgaatctg tagtttttac atattccaag atttgctttt
     3061 ttagatcaat gccgttgaaa tacataaatg ttattctttt gtcataaagc gtttagccat
     3121 tcttccccaa tcatttctaa cttcttgggc tttgtctttg atgttcccat ccggcaatgt
     3181 atcctcgtaa cgcgcccacg cttctagtaa caattttaat ccatcccttg cacgagataa
     3241 acgatcaact aaatcttcgc gtgacacatt tgtagtatct tcatttaata tttccattaa
     3301 attatcataa gcaggatgct cagtattgag agtaataatg attgttcccg ctttaggtct
     3361 aaccgtaaaa aatgcgggag tttgaatatc cgcatgtgca aaaacatatt ttaacccata
     3421 atctgttgct gttttagaaa tttcctttgc ttcttcttta tctagtccct gatcaactaa
     3481 tactttttct atttctgcaa tcctttcctt tacaggtaat ttttcaaaat catcattctt
     3541 agaagaataa ccctcttcaa taagttcgcg tgttactttt gttgcaataa cttcaggtga
     3601 atcttccgtc tgacgctctc cccgcgtaga atgagcacct gcattttgtg ccttgatatg
     3661 agctctcatt gcatgtaggt tttttttaat tctatttccc aaatcaagaa taggtgcaat
     3721 gggatcttct tctgcttcta attgttcttg atattcaata attgattgct taccttttgc
     3781 aatctcatca taatcgatct cagcaagatg agaaaaattt gttgcatatt gtttattatt
     3841 agttaatccg aatagttcgt ctagttctga tgggaattct aattctatcc cccaccacct
     3901 ttccattgga tcatactgaa ttgtccaaga tttatctagt tcaatctctc tatgtgctct
     3961 tacaatagat actccaatgt tccgagccgc atgcttccca tgttcacgac tacccgcccc
     4021 ttctcctttt cttgcttctt tctttgcgac gctaaatcga attgtaacag gatatttttt
     4081 gcctctataa tttacaggta gctttaattc cttatctcca taatcttcaa acattggagt
     4141 tttatcaaaa ggagcaggac aagatgtttt ttccattaaa tacattggat catttggcaa
     4201 agcatatcga tcaatcctta tatttatcaa atcatgtaga ttaaacgcta ccattctaat
     4261 agagcatttt tttttatcta aaaattttcg atataaccta ccaattaaga attctgagtt
     4321 atttataatt gcatctgctt ttctccaaag aattcgatct aaatttgacc aaacaactaa
     4381 cgttccagat ttaccaaact cttttcctat tctagaatat attttaggaa ttttatgttt
     4441 tttaggtttt ggtatttcta ttaactgttt attcttaatt tgatgtatat ctaaaaaagt
     4501 atacaaggca ttatcaacac catcctgcca ggaccaaact tctactaaag tgcattgcga
     4561 aatggaagaa gctggtaatc ccattccaaa ctttcctata ccttgctgat tctttgcatc
     4621 taaatgtgtt ccatttccaa attgtaagga taatcgtaaa acatcttcgt tcattccatg
     4681 cccatcatct aaaatagcga tttggctaat gcgtgttatc cggttattac gggcgccatc
     4741 aatttcttct tctccacata agagttgaac attctttgct cctgcctgta aagaattatc
     4801 tattagttcg gcaattgcat acgctgtatt tttgtaaccg ttatctctca tcgcttgaat
     4861 cgctaaatga ggaggaacta tatcatgtct atctgccata cctttctcct aattgtttac
     4921 aataatatat attaaaaaac atcttcccaa ttcataaagg cttctgccat acttctaaaa
     4981 cctggaagct ttgtatctac gactgtgaca atcagtgcat tatcattccc tccggcttta
     5041 ctaccccgaa ttgctctacc taccatctgt gaatataaca caagagattt agtcggtcgt
     5101 gctatcatag cacaactagt ttttggagca tcaaagccag tagtaaaaac cccataatta
     5161 cacaaaacaa ttgaagcttc cttatctgat ttatattctt ggattgcttt ataacgttca
     5221 ctatcaggcg tagctcctgt gactgaataa gactcaatac cgattgctcg taacataaaa
     5281 gctattaagg ctgagtgact aacggatgca gcaaagaaaa taattctttt atgataaagg
     5341 attaatgatt ttaaacgaga taaaattgca gtattacgaa gttcatcttg acctaaacga
     5401 attaaaattt cttcatttaa atctaactct ctttctatct ttgcaatatc ctcttgtgta
     5461 atagtatatc cacctttatg agttaacggt tcaaatgtta ctttcgctaa ataaccatta
     5521 tccgttaaat aatctatagg atttgaatat ccttcgatag ataaggtaat tttatttctg
     5581 tgaaaaaaat tggcgagttt ctcatcctca tcaatattta aataactccg accaggagtt
     5641 gccgttaatc ctataatcga aacgttttct tttcgactta atatttgtaa aattgtatta
     5701 taagattctg caatggcttg gtgtgcttca tccataacga ttaaagatat cttatcagat
     5761 aaatcattaa cgaattgaat actaccattg cgaattgtat tgtataattt tgataatcct
     5821 gctacaacta atccttctga tattccatct tgccactgaa tattagaata atatctttgt
     5881 atattgatgt ttctatttcc gagatatccc cacgcttttt taaattcact gactgcctgc
     5941 tcgcataatt cttcactata tgcaagccat ataaccaatg ctccttcgtt ttttcgcaaa
     6001 tagtctgcaa tatagttcat agtcgttcta gtttttccag aaccggtcg
//
LOCUS       DAOQOS010000215         6010 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_7784_length_6010_cov_5.849538, whole genome shotgun sequence.
ACCESSION   DAOQOS010000215 DAOQOS010000000
VERSION     DAOQOS010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 6010)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6010)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6010
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7784_length_6010_cov_5.849538"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..283
                     /locus_tag="PLF66_25250"
     CDS             <1..283
                     /locus_tag="PLF66_25250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67420.1"
                     /translation="ETATAASYFGYGVGGGITRYRVWKVPESKFFNLTFSSNLGNDLI
                     LVISAKNIFNQRVLYPLDQESGSFTSPTLDPRQLLGFGREMYFKLGYRF"
     gene            286..717
                     /locus_tag="PLF66_25255"
     CDS             286..717
                     /locus_tag="PLF66_25255"
                     /inference="COORDINATES: protein motif:HMM:TIGR04452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04452 family lipoprotein"
                     /protein_id="HMZ67421.1"
                     /translation="MKNQRIPIYLFLLILTFHACPITNKFVLKPSRVKGTEAKEIIQN
                     RLSSLFVNDISTGSNDSLVADFLIPTIAGINDTSIYSRRDVESCATKIFLGAIAIDQP
                     NIIANRTKRASAPLLQSNPNSRLYPPILCQLKPIDDWIDLD"
     gene            complement(718..1482)
                     /locus_tag="PLF66_25260"
     CDS             complement(718..1482)
                     /locus_tag="PLF66_25260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67422.1"
                     /translation="MTLILTIQNAYEYLEKLGPAKSIQLMKNLGYEKVISLSSKLKKE
                     RLIYLAQKLSDAKILELITQIPEKELIEIIEANSDEELIYYIHSLSMEDLKKIATSVA
                     PADIKKLAGGLGKESAIELVKNIGTDKSIGLIKYIGSDNLVKIIQDVNIEQVLPLVNA
                     LTLPQCKKWIKKRGLNEIPQLINALGAENMLTFIQSLGFEKSLDLMELLGFEKMVELT
                     HKISKMHFPHYVLENEEPKHHHKKHKGKTAAKIHAI"
     gene            1549..2802
                     /locus_tag="PLF66_25265"
     CDS             1549..2802
                     /locus_tag="PLF66_25265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67423.1"
                     /translation="MNNKKAFFERSKIVLNKISPVYFISGYLLLVSLLTYIKITPYDL
                     NLSSLIGLWQGFANLNSHLIDNNFIVFYDGGYDGQFFYLISKQLFMNGLENLPILDSF
                     FIRFNRLGLSLLAGTFSYVIGFQYYAIITLLVLHFIHLMAFILIRKILSNENKYLSLY
                     FLFSPFALNSNLLLVSDSLLASFIVFSIYALDKYTIIQVSQSTKKELSWSSLFLLLFI
                     LIFLCLIKESGIVILFSLLFVSLIENGTRNIFPILFSIILYLLIINLLKISINPNPGT
                     NPLSFLQLIDFPLFGFFKSIQFHPSMDIKSLFREMAKFPVFIFFILLTLNLLNIKTPR
                     KLFLSIPILFVLFTSGIGEVGYWLSFDNISRMFTISIPWIILLKEDDSAHNDYYALAF
                     SIILLLLLIIRIVYIKTPMRYYFFQ"
     gene            complement(2840..3400)
                     /locus_tag="PLF66_25270"
     CDS             complement(2840..3400)
                     /locus_tag="PLF66_25270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67424.1"
                     /translation="MIRLISKIFFLNLLIFVMNCASSQDSTQSDNKPATISSASNNPT
                     PELRSIPNAPMADDGWGTGYKKVIDGKEINLDGMWYTKKYSGKANERSIELKSPSFME
                     SSCKYSVLKGNSKTLVESALGSLGAQINPDKAKDLSKKIVKEQSKNFEISGCVPTSAD
                     KNFSECECYLSYKPEGGKEALIKYLN"
     gene            complement(3410..4276)
                     /gene="metF"
                     /locus_tag="PLF66_25275"
     CDS             complement(3410..4276)
                     /gene="metF"
                     /locus_tag="PLF66_25275"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004756496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HMZ67425.1"
                     /translation="MKISKIYSDAKSPVFSFEIFPPKTPEGDIKLINTVADLKKLNPS
                     FISVTYGAGGSTKEKTIDLCETIQNQFQITTMCHFTCVGASSSEIRDTLKVIESKKIE
                     NVIALRGDPPKGQGIFTKHPQGFGSGKELIEFIRNEKFTFCLAGGCYPEKHPDAPTLE
                     DDIKFLKQKVDAGAEFLITQLFFSNTLFQSFLSKARSAGINVPIIPGIMPITTLSQIN
                     RFKEMADCEIPQKLIDDLQNVKDDPTEFLKRSIQFTVEQCRELLKMGVAGIHFYTLNQ
                     STATIEIMKSLL"
     gene            complement(4278..>6010)
                     /locus_tag="PLF66_25280"
     CDS             complement(4278..>6010)
                     /locus_tag="PLF66_25280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="carbohydrate-binding protein"
                     /protein_id="HMZ67426.1"
                     /translation="KPNSQKKYVSQIAEMEIYADADIITLSKKRIIAETPPYSSIMRP
                     GLVRLAADGETKEGLVVQGNDRRLRDATTEFKGIVELATDGEDRELVVVQGNDKRLKH
                     ATENNYGLIRLAKKGESRPGLVVQSDDDRLKLATTDSPGIVELAEDGETRPGVVVQGN
                     DKRLKKATVSEYGLVIMSELGADTPGKVLTADDPRLKKATTEKEGIVRFASNGEESAM
                     SAVQGNDKRLKKATTELHGIVQLAQSGEDKEGVVVQGNDKRLKYASTENPGIIMLAKN
                     GLNTPNKAVAADDERLFDAREAKPHTHDYAPIVHDYNSHSGLIHLQGSSSSEFKNINP
                     PMPAHSVIYGKNEGKGGSGISGIGVDEGVIGFGDDNGVIGYSNGIDDDSAGVAGFSKK
                     GFGGVFSSQKKYALYANGIGIKKKDIVGAGKAILAHGESDFYGNVRLIDANGNDCIVR
                     YFKVTSDIVTKGDLVVLSDKEGSISKSRNAYSSKVVGVCVESASLELGEKKTGNEYAM
                     IALFGVVNLHVDAADGSILPGDMLVSGLAAGYAVKADPNKLKPGMLVGKALAEWKRDK
                     GIIPVILTLS"
BASE COUNT         1841 a         1170 c          845 g         2154 t
ORIGIN      
        1 tgagacagcg acagccgcat cttatttcgg ttatggagta gggggaggca taactagata
       61 tagagtttgg aaagtcccag aaagtaaatt tttcaacctt acattttcat ctaatttagg
      121 aaatgattta atacttgtta tttcagcaaa aaacattttc aaccaaagag ttctttatcc
      181 cctcgatcaa gaaagcggat catttacaag cccgacgctt gaccctcgcc aactacttgg
      241 atttggaagg gagatgtatt ttaagttagg atataggttt taattatgaa aaatcaaaga
      301 attcccattt acctattttt acttatatta acgtttcacg cttgcccaat tacaaataaa
      361 tttgtattaa agccatcccg agtaaaagga acagaagcaa aagaaatcat tcaaaacaga
      421 ctctcatctc tttttgtaaa cgacatctcc acaggaagta acgactcatt ggtggcagat
      481 tttttaatac caacgattgc aggaattaat gatacaagca tctacagtag aagagatgtt
      541 gaaagctgtg ccactaaaat atttctaggt gcaatcgcta tagatcaacc aaacattatt
      601 gcaaatagaa caaaaagagc atctgcccct ttactacaaa gcaatccaaa cagtcgactt
      661 tatccaccta tactctgcca attaaaacca atagatgact ggatcgactt agattaatta
      721 gattgcgtga atctttgcag ctgtttttcc tttgtgtttt ttgtggtgat gctttggctc
      781 ctcattctct aaaacataat gaggaaaatg catcttggat attttatgag ttaactccac
      841 catcttctca aagcctaaca attccattaa atctaaactt ttctcaaaac ctagactttg
      901 aataaatgtt aacatattct cagcacctaa cgcattgatt aattgaggta tctcatttaa
      961 tcctcgcttt tttatccatt ttttacattg aggtaaggtc agagcattta caagcggtaa
     1021 aacctgttct atatttacat cttgtataat ttttactaaa ttatctgatc ctatatattt
     1081 aattaaccca atagatttat ctgttccaat attttttact aattcaattg cactttcttt
     1141 tccgagtccg ccagcgagtt ttttaatatc ggcaggcgca acagacgtag caattttttt
     1201 cagatcttcc atagataaac tatgaatata ataaattaat tcttcatcag aattcgcttc
     1261 aataatttca attagttctt tttcaggaat ttgggtaatt aattctagaa tttttgcatc
     1321 agaaagtttc tgcgctaagt aaatcaatcg ctcttttttt aatttcgaag atagcgaaat
     1381 cactttttcg tatcccaaat tcttcatcaa ttgaatagat tttgcaggac ctaatttttc
     1441 aagatactca taggcattct gaattgttaa tatcaaagtc atagctattc caattctata
     1501 ggggtattta ttcttgcaat caaattaaat attgaaaata gtagagaaat gaacaataag
     1561 aaagcatttt tcgaaagatc aaagatagtc ttaaacaaaa tcagtcctgt ttactttata
     1621 agcggatact tattgttagt ctctctttta acttatatca aaattacccc gtatgatttg
     1681 aatctatctt ctttgattgg actatggcaa ggctttgcaa atttaaattc tcacttaata
     1741 gataataact ttattgtgtt ctatgatgga ggatatgacg gtcaattttt ctatttaatc
     1801 agcaaacaat tatttatgaa tggactagaa aatctaccaa tattagactc tttctttatt
     1861 cgatttaatc gattgggatt atctttatta gctggaactt tttcttatgt aataggcttt
     1921 caatattatg caataataac cttacttgtc ttacacttca ttcatctaat ggcatttatt
     1981 cttattcgaa aaatattatc caatgaaaat aaatatttat ctctgtattt tcttttttct
     2041 ccctttgcat taaactcaaa tttactccta gtctctgact ccttactagc atcatttatt
     2101 gttttctcca tttatgcgtt agataaatac acaatcattc aagttagcca atctactaaa
     2161 aaagaattga gttggtctag tttattcctt ctacttttta tattaatttt tctttgctta
     2221 attaaagagt caggaattgt aattctattc tctcttttat ttgttagttt aatagaaaat
     2281 gggactagaa atatttttcc aatactgttc tctataatat tgtatttact tataataaat
     2341 ctattgaaaa tatcaataaa cccaaatccg ggaacaaacc cactctcttt cctacagtta
     2401 attgatttcc ccttatttgg tttttttaaa agcattcaat ttcatcctag tatggatatc
     2461 aaaagcctat ttagagaaat ggcaaaattt ccagtattca tcttttttat tttattaaca
     2521 ctgaatctat taaatattaa aactcctcgg aaattatttc tatcaatacc tattttattt
     2581 gttctattta catcgggaat aggggaggtg ggttattggt tatcttttga taatatctca
     2641 agaatgttta caattagtat tccttggatt attctactaa aagaagatga ttctgcgcat
     2701 aacgattatt atgcgcttgc atttagcatt attttacttt tactcttaat tattagaatt
     2761 gtttatatca aaacacctat gcgatattat ttttttcaat aaattaactc aaccaagtat
     2821 gaatcctttt ttggatcttt tagtttagat actttatcaa tgcttctttc ccaccttccg
     2881 gtttataaga aagatagcat tcacattctg aaaaattttt atctgcagaa gtaggaacgc
     2941 aaccagaaat ctcaaaattc ttactttgtt cttttacaat tttcttagat aaatctttcg
     3001 ctttgtcagg attgatctga gctcctaagg atcccaatgc agattctacg agtgttttgg
     3061 aatttccttt taaaactgag tatttgcaac tagattccat gaaagacgga gattttaact
     3121 ctatagatct ctcattggct ttgcctgaat atttcttagt ataccacatc ccatccaaat
     3181 ttatttcttt cccatcaatg acttttttat atccggttcc ccatccgtca tccgccatag
     3241 gagcatttgg aatggaacga agttccggag tagggttatt gctagccgat gaaatagttg
     3301 ctggcttgtt atcagattga gtcgaatctt gagaactagc acaattcatc acgaagatca
     3361 gtaaattcaa aaagaatatt ttagatatta atctaatcat ttaaaactcc tataataaag
     3421 atttcattat ttctattgtg gctgttgatt gatttaatgt ataaaagtga atgcctgcca
     3481 ctcccatctt aagtaactct ctgcattgtt ctactgtaaa ttgaatgctt ctcttcagaa
     3541 actctgttgg atcatccttc acattttgta aatcatcaat taacttttga ggaatctcgc
     3601 aatctgccat ttccttaaaa cggttaattt gacttaaagt agtaattggc ataataccag
     3661 gaataatagg aacgttaatc ccagcacttc ttgctttaga taaaaaactc tgaaaaagtg
     3721 tattagaaaa aaacaattgt gtaattaaaa actcagctcc cgcatccacc ttttgtttta
     3781 aaaatttaat atcatcctct agagtagggg cgtctggatg tttttcggga taacacccac
     3841 cagcaagaca aaaagtaaat ttttcattcc ttataaactc aattaattct tttccacttc
     3901 caaaaccctg cggatgcttt gtaaatatac cctgtccctt agggggatca cctcttaaag
     3961 ctatgacgtt ttctattttt ttagattcaa tcacctttag agtgtctctt atttcggaac
     4021 tactagctcc tacacaagta aaatgacaca tagtcgtaat ttggaattgg ttttgaatgg
     4081 tttcacacaa atcgattgtt ttttcttttg ttgaaccacc agctccataa gtcacagata
     4141 taaagcttgg atttaatttt tttaaatcag caaccgtatt tataagcttt atatctcctt
     4201 caggagtctt tggaggaaaa atctcaaacg agaaaacagg agattttgca tccgaatata
     4261 ttttcgaaat tttcatatta actcaatgtt aggataactg gtattatccc cttatcacgc
     4321 ttccattcag ccagagcttt accaacaagc attccgggct tcaacttatt tggatctgct
     4381 tttactgcat accctgctgc caaacctgag actaacatat cacctggaag aatagatcca
     4441 tctgcagcat ctacatgtaa atttactact ccaaagagag cgatcattgc atattcatta
     4501 cccgttttct tttctccgag ttccaaactt gcggactcca cgcaaacacc tacaaccttt
     4561 gaagaataag cgttacgcga tttagaaata gatccttctt tatctgataa tacaactaaa
     4621 tcacctttag taacaatatc actcgtaact ttaaaatatc ttactataca atcatttcca
     4681 tttgcatcta tcaaacgaac atttccataa aaatcagatt ccccatgagc aagtattgct
     4741 ttaccagctc ctacaatatc tttcttttta atgccgatac cgtttgcata tagtgcatat
     4801 tttttctgtg aagaaaatac tcctccaaaa ccttttttgg aaaatccagc tactccagct
     4861 gaatcatcat caatgccgtt cgagtaacct ataactccat tatcatctcc aaaaccgata
     4921 actccttcat ctacaccaat tccacttatt ccagaaccac cctttccttc attttttcca
     4981 tagataacag aatgagctgg cataggagga tttatatttt taaactcaga ggaagatgag
     5041 ccttgtaaat gaattaaacc tgaatgggaa ttataatcat gaacgatagg tgcgtaatcg
     5101 tgagtatgtg gctttgcttc tcttgcatca aacaatcttt catcgtcagc cgctacagct
     5161 ttatttggag tattaagacc attcttagca agcataataa taccgggatt ttctgtagaa
     5221 gcatacttca acctcttgtc attgccttgc acaacaacac cttctttgtc ttcgccagat
     5281 tgagccaatt gaactatacc atgcaattcg gtagttgctt tttttaatct cttatcatta
     5341 ccttgcactg cactcattgc tgactcttct ccattgcttg caaatcgaac aatgccttct
     5401 ttttcagtcg ttgctttttt taatcgtgga tcatctgctg ttaaaacttt tccaggagta
     5461 tctgctccta actcagacat aatcactaga ccatattcgg atactgtcgc cttttttaag
     5521 cgcttatcgt ttccttgaac aactacgcct ggtcttgttt caccatcttc tgccaattca
     5581 acaatgccag gagaatctgt tgttgctagt tttagtctat catcatcact ttgcacgact
     5641 agacctggtc ttgactcacc cttttttgca agacgaatca atccatagtt attttcagta
     5701 gcatgtttca atcgtttatc atttccctga acgacaacta attccctatc ttcaccatct
     5761 gttgcaagtt caacaatgcc tttaaactct gtagtcgcat ctctaagtcg tctatcgttt
     5821 ccttgcacca ccaaaccttc ttttgtttca ccatcagcag ccagtctaac taaacctggc
     5881 ctcataatag aagagtaggg tggggtttct gctataattc tttttttaga taaagtgatt
     5941 atatcagcat cagcataaat ctccatttct gcgatttgag aaacatattt cttttgggaa
     6001 ttgggtttat
//
LOCUS       DAOQOS010000216         5917 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_7977_length_5917_cov_5.760321, whole genome shotgun sequence.
ACCESSION   DAOQOS010000216 DAOQOS010000000
VERSION     DAOQOS010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5917)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5917)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5917
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7977_length_5917_cov_5.760321"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            295..2853
                     /locus_tag="PLF66_25285"
     CDS             295..2853
                     /locus_tag="PLF66_25285"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HMZ67427.1"
                     /translation="MNIKIIFALFFFHLNLFAVETINLVKDCDSSKLCKQHEWQIIDS
                     FQEEYISLNKLPDNAKIIEKFPITIKTIYPDTKSNDFTLKTSFSISEDYLKETEIKGL
                     YLEAIADVYAIYLNGKLIKKEGKFENGKVVYSRYGKKLAYPLSTNFFKLGKNDLTIHI
                     QGEPKFNLLGLFSANGYYLDSFEKVSVESRDRTGIILFFLYLFVGAYHLFLYIKRNNE
                     KYNLFFSLASLFIFIYLMSRSGILFEYGLDSTIGYKIELLSVYYILPCLGIFFELLFF
                     NKIGKPLNIYSLYCILLSFITIFLPQHLILYVLRAWQITALCCLFYFFFIFYKAIKAK
                     SNSAKRLLIGVTILILATIFDVLDSIIFKLNLGLTRYAVFGFIVGIVGLLANRFINLH
                     ETVEDLNKTLEDKVEQRTKELQDTLKEVKLLKEQQDGDYFLTTLIISPLASNVQGDDT
                     VDIQILIHQKKRFEFKNKIHEIGGDICISETIYLKGRKFTAFVNGDAMGKSIQGAGGA
                     LVLGVVFKSILSRAKLYSTKEQVFPERWLKLAFQELQSVFESFDGSMMISVVMGLIDN
                     EKGLLYYINAEHPFCVLLRDNKASFIEKESSIRKIGMMTLEHSGNFQVNTFKLEPNDI
                     VIIGSDGRDDIKLSENESGRQINEDESLFLRTVEEAKGNLENIFSLTEKKGELIDDFS
                     LLRISFKKTPDGILIEETKESIEALEAYNSGNLGRVEVLVDTAIQQNKINKTILKLQA
                     NIAYQRKLYSKAAEFYAKYLELFPDENQFLFACAKSFKLAKLYELAADYSERLKLREP
                     KNSSNLVLLVEIYCDLKAFTRAEKILDQLKSVELENPKVEELKRLIEDGKKVDS"
     gene            complement(2867..4438)
                     /locus_tag="PLF66_25290"
     CDS             complement(2867..4438)
                     /locus_tag="PLF66_25290"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HMZ67428.1"
                     /translation="MYLPRKLLTRSLFVLLFSLLISTHLFSFGRNEAKKAFDAVRDAI
                     KLYVYCKDCGDLKARVLFINERGVEPIIKDKQVDYQFFVNKKQYLNIHDIYFQKNNQW
                     INLGIYSGIQNSTIPYELPADKMPFDLEAESEKNFKTPSDIFPLTIFEKQILEELNKV
                     RTSPKEYAKSLRERIPFYENNIYYPLDRRPYQTKEGIKGLEEAIANLELAQPRSPLRI
                     SAEISSALQNYFKSNFSYSSHWRELRDRYGKYIQDPELINIVESQVFGFNNPSDLVAY
                     LLSMNTRKKGEFKNSIFDTDINYLGLACRNDSTYTYMCHLTLAEDIIPNVEMQEYQKD
                     RNKKSNYNYLSEFEYKVIEETNLLRTKPREYAEIIKRRRVNYFDKIYKEISRSPITVE
                     EGISALEEAIRFLEKVTPLSELTPNEELTLSAKDHVLDTGSKGITGHYGSSGSSPVNR
                     IQKYKKEYKIVGENIYYGTVDPRDVVISLLVDDGVSNRGHRANLFHKDFNYIGVSCGY
                     HRDFPLMCVQNFGNW"
     gene            complement(4506..5678)
                     /locus_tag="PLF66_25295"
     CDS             complement(4506..5678)
                     /locus_tag="PLF66_25295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010576011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1577 domain-containing protein"
                     /protein_id="HMZ67429.1"
                     /translation="MQYIDKKSRDIDVITSLEQKHHILSKYLLDKELLLRMDPFDQKV
                     ILKKILEGGEKILVYLPPETDFFFPMNVQLFKILANYIHIDCDFISQVEDGLFLLQVN
                     KLEIARRNRENERIPLPSGTAFATNIISNKFLIQSNMFNVPNLVKVHFDDYESRLSQR
                     FDCNISVNVFKSDLPLSYDVVRKTQKFLWLPDTQNPRSYVAYFADQINYRDEIDDDIS
                     HAIAQYKEQKIVSELILPILYTNQGDEKISLGYISIQSKDKKISQTMLEDLKKVRVEI
                     ARRIQESNTVKTQERFPILEVSRHGLRMSITNPYLIESLQKHDRFVFDIYFRMQAPLT
                     VSGIIRWISKNEEGTRLDLGVEIVGKSALAGERERYHKNIDLARQGKLQLSSSSKS"
BASE COUNT         2017 a          932 c          875 g         2093 t
ORIGIN      
        1 aatacttcaa tatacgataa ccgccataat tcttatctag aattagattc actctaattt
       61 ttttgattat gaattgtatt tctatacaag tcgatggtct aaaagaaaaa acaaaagata
      121 aattgaaaga atgcaatatt ctatcactac agtttagtaa ttgaaattat ttcctaatgg
      181 attatcgttc tctattcatt caatgtgttg aataatttat acatttagaa aaactgtctt
      241 gacccatttg ttatcaattg ttgtgatttg ttatattttt tcaaaaggat tcatatgaat
      301 attaagatta ttttcgctct ttttttcttt catctaaatt tatttgcagt ggaaaccatc
      361 aatctagtaa aagactgcga ttcatccaag ctttgcaaac aacatgaatg gcaaatcatt
      421 gattcctttc aagaagaata tatttcctta aacaagttac cagataacgc taaaataatc
      481 gaaaaatttc ctattactat aaaaactatt tatccagata ctaagagcaa tgattttact
      541 ttaaagacta gcttttccat ttcagaggat tatttaaaag aaactgaaat caaaggtcta
      601 tatctagaag cgattgctga tgtatatgca atttatttaa atggtaaact gattaaaaaa
      661 gaaggaaaat ttgaaaatgg caaagtagtc tacagtcgat atggaaagaa attggcttac
      721 ccacttagca ctaacttttt taaacttgga aaaaatgatt taactataca tattcaggga
      781 gaaccaaagt tcaatttatt aggactcttt tcggctaacg gatattatct agacagtttt
      841 gaaaaggtat ctgtagaaag cagagataga acaggaatca ttctattttt tctctattta
      901 tttgtaggag cttatcatct atttctatat atcaaaagaa ataatgaaaa atataacctt
      961 ttcttttcac tagcttctct ctttattttt atttatctaa tgagtagaag tggaatttta
     1021 tttgaatatg gtcttgactc aacaattggt tataaaattg aattattatc tgtttattat
     1081 atcctacctt gccttgggat cttttttgag ttattatttt ttaataagat tggcaaaccc
     1141 ctaaatattt actcccttta ctgcattctt ctttctttta taactatttt tttaccgcaa
     1201 catcttatac tctacgtact cagagcatgg caaataacgg ctctatgctg cttattctat
     1261 ttctttttta tattttataa ggcaattaag gctaaatcaa attctgcgaa aagattactc
     1321 attggagtca ctattttaat attggctaca atttttgatg ttttagattc tattatattt
     1381 aaattaaatt taggactaac taggtatgct gtattcggtt ttatagttgg tatcgtaggg
     1441 ctactcgcga atcgttttat taatctacac gaaacagtag aagacctaaa caaaactctt
     1501 gaggacaagg tagagcaaag aacaaaagaa ttgcaggaca cattaaaaga agtaaaactt
     1561 ctaaaggaac aacaagatgg agactatttc ctaacaactc taatcataag cccattagcg
     1621 tcaaatgttc aaggagatga cactgttgat attcaaatat taatacatca gaaaaaacga
     1681 tttgaattta aaaataaaat tcatgaaatt ggtggagata tttgtatatc tgaaaccatt
     1741 tatttaaaag gaagaaagtt cacagccttt gtaaatggag atgctatggg aaaatcaatc
     1801 caaggtgcag gtggtgcgtt agttttaggc gttgttttta aatctattct ctcaagggcg
     1861 aaattatatt ctacgaaaga acaagttttt ccagagcgtt ggctaaaatt agcttttcaa
     1921 gaattgcaaa gtgtctttga atcctttgat ggatctatga tgatatcggt cgttatgggt
     1981 ttaatagaca atgaaaaagg tctcttgtat tatataaatg cagaacatcc attctgtgtt
     2041 cttctgagag ataataaagc aagctttata gaaaaggaat caagtatccg taaaattgga
     2101 atgatgactc tagagcactc tggaaatttt caagtaaata cttttaaatt agaaccaaat
     2161 gatatagtaa ttatagggtc tgacggtcga gatgatatta agcttagtga gaacgaatcg
     2221 ggaaggcaaa taaacgaaga tgaaagtctc tttctaagaa cagtcgaaga agcaaaagga
     2281 aatctcgaaa atattttttc tttaacagaa aaaaaaggcg aattgattga tgattttagt
     2341 ttacttagaa tttcttttaa gaaaactcca gatgggattt taatcgaaga aacaaaggaa
     2401 agcatagaag ctttggaagc gtataattct ggaaatttag gtagagtgga agtcttagta
     2461 gatacagcca ttcaacaaaa taaaataaat aaaacgattt taaaattaca agcaaacatt
     2521 gcataccagc gaaaattata ttcaaaagca gctgaatttt atgcaaaata tttagaatta
     2581 tttccagatg aaaatcagtt tttatttgca tgtgccaaaa gttttaaatt agcaaaacta
     2641 tatgaactag cagcagatta ttcggaacga ttgaagttaa gagagcctaa aaattcctct
     2701 aacttagtat tactagtaga aatatactgt gatttaaaag ctttcacgcg agcagagaaa
     2761 attttagatc aactcaaatc tgttgaattg gaaaatccca aagttgaaga actaaaaaga
     2821 ttaatcgagg atggaaaaaa agttgattct taaaccattc aaacgattac cagtttccaa
     2881 aattttgaac acacattaaa ggaaaatccc tgtgatatcc acatgataca ccaatgtaat
     2941 taaaatcttt gtgaaataaa ttggctcgat gccccctatt agaaacacca tcatccacta
     3001 gtagggaaat cacaacatct cttggatcaa ctgttccata ataaatattc tctcctacaa
     3061 tcttatattc ttttttgtat ttttgaattc tattcaccgg agaagatcca ctactaccgt
     3121 aatgcccagt gatccctttt gatcctgtat ccaatacatg atcttttgcg gataacgtta
     3181 attcttcgtt tggagttaat tcgctcagag gcgttacctt ttctaaaaat cgtatcgctt
     3241 cttctaaggc tgaaattccc tcctctacag ttatcgggga tcgacttatt tctttatata
     3301 ttttatcaaa gtaatttact cttcttcgct ttattatttc agcatactct ctaggttttg
     3361 ttcttaatag atttgtttct tcaattactt tatattcgaa ctcggagaga tagttgtagt
     3421 tggacttttt atttctatct ttctgatatt cctgcatttc aacatttgga attatatctt
     3481 cagcaagcgt taaatgacac atatatgtat atgttgaatc attcctgcag gcaagaccta
     3541 agtaatttat atctgtatca aatattgaat ttttaaattc tccctttttt cttgtattca
     3601 tagaaagtaa ataagcaacc aagtcagaag gattattaaa accaaatact tgtgattcta
     3661 caatattaat tagttcggga tcctgtatgt attttccata tctatctctc aactctcgcc
     3721 agtgactaga ataggagaaa ttagatttaa aataattttg caaagcactt gatatttccg
     3781 ctgaaatcct taatggactc cttggttgtg ctaattctaa atttgcaatt gcctcttcta
     3841 atccttttat tccttctttt gtttgatatg gtcttctatc tagcggataa taaatattat
     3901 tctcataaaa cggaattcgt tcccgaagac tctttgcata ttcttttggt gaagttctca
     3961 ctttatttaa ctcttctaga atctgttttt caaaaattgt aagcgggaaa atgtcggagg
     4021 gtgttttaaa atttttttct gactctgcct ctaaatcaaa aggcatctta tcagctggca
     4081 attcataagg aatagtagag ttttgtattc cagaatagat tcctaaatta atccattgat
     4141 tgtttttttg aaaatatata tcgtgaatgt taagatattg ctttttattt acaaaaaatt
     4201 gataatcaac ctgcttatct ttaatgatag gctcaacccc tctctcattg atgaataaaa
     4261 cacgagcctt taaatctcca caatctttac aatatacata aagtttaatt gcatccctca
     4321 ctgcatcaaa agccttttta gcttcatttc tcccaaaact aaataggtga gtagaaatga
     4381 gtaaggaaaa aagtaagaca aataaagatc gagtgagtaa ctttctaggt aaatacatac
     4441 tatccttata aaattagatt tgtatgtatg tctattttaa attcgccacc acaagtttga
     4501 atctatcaag attttgagga actagataat tgaagctttc cttgtcttgc taagtctata
     4561 tttttgtgat aacgttccct ttctcctgcc aatgcagatt ttccaacaat ctcaactcct
     4621 aaatcaagcc gagttccttc ttcatttttc gatatccacc gaataattcc tgagacggtt
     4681 aaaggagctt gcattctaaa atatatatcg aaaacaaaac gatcatgttt ttgtaaactt
     4741 tcaattagat agggatttgt aatgctcatt cgaagcccat gtctagatac ttccaaaata
     4801 ggaaatcttt cttgtgtctt cactgtattt gactcttgaa ttcgtctagc aatttcaact
     4861 cttactttct ttaaatcttc gagcatagtt tgagaaatct ttttatcttt actttgaatt
     4921 gagatataac caagacttat tttctcatct ccttgatttg tatataaaat tggaagaatt
     4981 aattcagata caattttttg ctctttatat tgtgcaatcg catgcgaaat atcatcgtct
     5041 atctcatctc tataattgat ttgatctgca aaataggcta cgtaggatct agggttctga
     5101 gtatctggaa gccatagaaa tttctgtgtc tttcggacaa cgtcatacga taaaggcaag
     5161 tcagacttaa aaacattcac tgaaatatta caatcaaatc tctgtgataa cctactttca
     5221 tagtcatcaa aatgaacctt aactaaattt ggaacgttaa acatattgga ttgaattaga
     5281 aatttatttg aaatgatatt tgtagcaaaa gcggttccag aaggaagagg aattctttca
     5341 ttctctctat ttcttctcgc aatttctagt ttgtttactt gcaataaaaa caaaccatct
     5401 tccacttggc ttataaagtc acaatcaata tgaatataat tggctaggat tttaaaaagt
     5461 tgaacattca tcggaaagaa aaaatctgtt tcaggcggaa gatagactaa aatcttttct
     5521 cctccctcta atattttctt aagaatcact ttttgatcaa atggatccat tcttaatagt
     5581 aattccttat ctaaaagata cttggataag atatgatgct tttgttctag ggaagtaatc
     5641 acgtcaatgt ctctcgactt cttatcaatg tattgcatag tcttgtttta ggtctaaaac
     5701 tcctttgcct tttttatttc taaactaacc agtcccaatc ctactatttt aagaatttat
     5761 ccaaattagc agcagaaaag aaccttttgt tttgtcttac cttatttagt aaatttattg
     5821 acaaagggtc aataataaat tgacaaattt aagttttagc aggaaatttt tttactaaaa
     5881 atatattaaa atatataata ctataaaaaa aatttac
//
LOCUS       DAOQOS010000217         5784 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_8281_length_5784_cov_4.811311, whole genome shotgun sequence.
ACCESSION   DAOQOS010000217 DAOQOS010000000
VERSION     DAOQOS010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5784)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5784)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5784
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8281_length_5784_cov_4.811311"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(179..538)
                     /locus_tag="PLF66_25300"
     CDS             complement(179..538)
                     /locus_tag="PLF66_25300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67430.1"
                     /translation="MIIKRQILPTDLESKALNLGKLLKDRNISLDGINGDSILSSITS
                     IENDQAEIQRKEHELRDFKEKLKSKREQLYNDFRVTSDYINSRSRNEPELKGMLSSFK
                     VSKKKRKKVDSETQVAA"
     gene            1666..3213
                     /locus_tag="PLF66_25305"
     CDS             1666..3213
                     /locus_tag="PLF66_25305"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002976618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HMZ67431.1"
                     /translation="MTKTKEKKANEEPIEKQLWKAADKLRKNIDAAEYKHIVLGLIFL
                     KYISDAFEELHTKLKSGKGEYAGADPEDKDEYKAENVFFVPEKARWSYLQSKAKLPVI
                     GKEVDNAMDAIEKDNPSLKDVLPKVFARGNLDPTSLGGLIDLVGNIALGDAKARSADV
                     LGHVFEYFLGEFALAEGKKGGQFYTPRSVVELLVEMLEPYKGRVYDPCCGSGGMFVQS
                     EKFISEHQGKINDISIYGQESNQTTWRLAKMNLAIRGIDSSQVKWNNEGSFLNDAHKD
                     LKADFVIANPPFNDSDWSGNTLRKDGRWKYGVPPEGNANYAWIQHFLYHLSPNGNAGF
                     VLAKGSLTSKTSGEGDIRAELIKARLIDCVVNLPAKLFLNTQIPACLWFLNRNKPAAR
                     RDEVLFIDARNLGFLINRRTKELSKEDIQQIAATYHNWKLGKDAKGKKKYEDIKGFCN
                     SASIDRIHELDYVLTPGRYVGLPDDEDDFDFKERFTSLQKEFEEQLKEETKLNKLILE
                     NLKKVKV"
     gene            3220..3537
                     /locus_tag="PLF66_25310"
     CDS             3220..3537
                     /locus_tag="PLF66_25310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67432.1"
                     /translation="MNSEKLIMNNAHIHILAKELVRVKKQAAALGMFTNDRELLECNR
                     CGLVEDVTIQNILITYFKNEKEDALTSLDSGLRFTETNDPNIFVCPSCGNNVELPVED
                     LIV"
     gene            3850..4380
                     /locus_tag="PLF66_25315"
     CDS             3850..4380
                     /locus_tag="PLF66_25315"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HMZ67433.1"
                     /translation="MQERKLNRLSGYDYSTAGYYFITSCVKNRECLLGEIFNSKMDLN
                     EFGKIVNTCWLDLPNHYKNIQLDEFVIMPNHFHGIIYIMGNGDVIDVGNGFKPFPTLA
                     MKPFPTGKNQGLPEIVRGFKTFSSRRINETNPNQKFQWQKSYHDRIIRNERELFLIRR
                     YIRNNPMNWMKDKHYI"
     gene            4563..>5784
                     /locus_tag="PLF66_25320"
     CDS             4563..>5784
                     /locus_tag="PLF66_25320"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HMZ67434.1"
                     /translation="MNEWKETDLGTIPNHWLVVNVDYLIEHKIIEKPLDGNHGELHPS
                     GNDFVDKGIPFVMATDVVNGKVDLANCKFITEEQAGKLNKGFSLPGDVLLTHKASIGR
                     TAIVGKITSPYIMLTPQVTYYRVINENKLLNKYLKYFFDSPVFQEILINHSDSGSTRA
                     YIGITAQRELPILLPPLPEQRAIAAVLSSLDDKIDLLHRQNKTLEAMVETLFNEQFIM
                     NNEEWKIGQVSDFIEFNPTRSLPKGTIAPYLEMANVNTSVFHPAGWYDREFSSGMKFI
                     NGDTLLARITPCLENGKSAYITFLNENQVGWGSTEFIVMRSKENLHTLFTYALARNKD
                     FRDYAEGCLEGSSGRQRVNIDHLIKFEINVPPKENIERFNSLMENIEPKLHNNFIQIQ
                     TLTKLRDTLLPKLMS"
BASE COUNT         2020 a          921 c         1077 g         1766 t
ORIGIN      
        1 aaactccatt gccgctcgtt cgcggcaata ggtggtagtc agttttaaca tacttgtggg
       61 taacgttatt ttgcgaaagg gggcttagga gaaggagtat ttttttgtag aaaaggatga
      121 cgttaattaa atagaataga ctcaaaccct agtatttgag tctattcttc atttggattt
      181 aagctgcgac ttgggtctca ctgtctacct tctttctttt tttcttagaa accttgaagg
      241 aagatagcat cccttttaac tctggttcat ttctagaacg agagttaata tagtctgagg
      301 taactcgaaa atcattataa agctgttcgc gtttgctttt cagcttttct ttgaaatcgc
      361 gtagttcatg ctctttgcgc tgaatctctg cttggtcatt ttctatggaa gtgatactgg
      421 aaagtattga atctccattg attccatcta aactgatgtt tctatccttt agcagtttgc
      481 ctaagttaag cgccttgctt tccaagtctg tgggtaatat ttgccttttt ataatcataa
      541 aaagacctcc aaaattatta tttggctaat agccattact agtatagacg gttcttgtga
      601 tatgcaatat gacataacgc attaaaaaag atgaaaaaac ttaaaaaaaa gttagcatat
      661 tattgatatg taaaaatata caatacgtat aaagtatatc gtattaatat aaataaacaa
      721 ttatttatat taataactaa gaaatatact taaggttatt ttatagatta aatattaatt
      781 ttattaactc accgtaaatg aggtttgctc ctagcggagg ccttgccgcc gcatgctatt
      841 gattttatta attcattagc attctccaga tttttaaaat catcatttat tcctcaattt
      901 ataattttta cctaagatga attattaaat tataataaga atagtttggg aatctcgtca
      961 gtttacggta aatgatataa acgaaataga atagaaaatg attgtctaga ataaatacgg
     1021 aaaaatgtcc gtgaatatcc taaatctata aatgaattta tttttattct ctgaaatcga
     1081 attgttttcc taattgttaa atccgaacct ttgaatctct tttctaaaat tatatctatc
     1141 gttatcatat tcagaagaaa aattattaat aaatcaatat cttgtttcat tatttgagct
     1201 tgttattatg cgaattagaa aatgaattat tgctaaatca gtcgctcttt ttgttattta
     1261 aagttaccgt tagcttaatg agagaataga ttttgaataa ctcagcttat cactttgcca
     1321 atagaatcta tcactacgct aatgaggaaa tgaattatca ttgattgaat tccaggttcc
     1381 cgtctctgat cctaccgtaa ccaaaataga aaagtcaaat ctgattaaga ggacggaact
     1441 tagacctaat tcaaagtatc attaggatat tcaaaccgat cctttgtgaa atggaaaaat
     1501 tactttctgt taataggttc taccattccg atttttgaaa atcactacta gcaaagttta
     1561 gtttccctat tgacaagatt aatactatga attatttcta atttgtccca gagtcatgat
     1621 acagtgataa actgagtatt ttaaaagaga gtaaaaggat tttaaatgac taaaaccaaa
     1681 gaaaagaaag caaacgaaga accaattgaa aaacagcttt ggaaagcggc ggataagctt
     1741 agaaaaaata ttgatgccgc agaatacaaa catattgttc ttggacttat atttttaaaa
     1801 tacatttcag atgcatttga agaattgcat acaaaattaa agagcggcaa aggcgagtat
     1861 gccggagctg atccagaaga caaagacgaa tacaaagcag aaaatgtttt ctttgttcct
     1921 gaaaaagctc gttggtctta tttacaatcc aaagcgaaac ttccagttat tggaaaagaa
     1981 gtcgacaacg cgatggatgc gattgaaaaa gacaatcctt ccttaaaaga tgttctccca
     2041 aaagtatttg ctcgtggaaa tttagaccct acaagtcttg ggggactcat tgaccttgtg
     2101 ggtaacattg cgttaggcga tgcaaaggct agaagtgcag atgtgcttgg tcatgtgttc
     2161 gaatatttct tgggcgaatt tgcattagcc gaagggaaaa agggtggtca gttctataca
     2221 cctagaagtg ttgttgagtt gcttgtagaa atgttggaac catataaagg tcgtgtgtat
     2281 gatccttgct gtggttctgg tggaatgttt gttcaatcgg aaaaatttat ctctgagcac
     2341 caaggaaaga taaacgatat ttcgatttat ggtcaagaga gcaatcaaac aacttggcga
     2401 ctagctaaga tgaatcttgc aattcgagga atcgatagtt ctcaagtgaa atggaataat
     2461 gaaggctctt ttttaaacga tgcacataaa gatttaaaag ccgattttgt aatcgcaaat
     2521 cctcctttca acgatagtga ttggagtgga aatacacttc gtaaagatgg acgttggaaa
     2581 tatggcgttc ctccagaggg taatgcaaat tatgcgtgga ttcaacattt cctctatcat
     2641 ctcagcccaa acggaaacgc agggtttgtt ctagcaaaag gatcgttaac ctcaaaaaca
     2701 tctggcgaag gcgatataag agcagaatta ataaaagcaa gactcattga ctgcgttgta
     2761 aatcttccag caaaattatt tttaaatacg caaattccgg cttgtctctg gtttttaaat
     2821 cgaaataaac ctgcggcaag aagagacgaa gttttattta tagatgcacg taaccttgga
     2881 tttctaatca atcgtagaac caaagaatta tccaaagaag atatacagca aatagccgct
     2941 acctatcaca attggaaatt aggcaaggat gcaaaaggaa aaaagaaata cgaagacatc
     3001 aaaggttttt gtaattccgc aagcattgac cgaatccatg aactagatta tgtattaacc
     3061 cccggacgtt atgtaggtct tcctgatgac gaagatgact ttgatttcaa agaaagattc
     3121 acctccttac aaaaagaatt tgaagaacaa ttgaaagaag aaacaaaact caacaaattg
     3181 attttagaga atctgaaaaa ggtgaaagtg tagaagagaa tgaatagtga aaaattaata
     3241 atgaataatg cgcatattca catattggcc aaagaattag taagagtgaa aaaacaggcg
     3301 gcagctttag gtatgttcac gaatgacaga gagttattag aatgcaatcg ctgtggttta
     3361 gtggaagatg ttacgattca gaatattctc attacatatt ttaaaaatga aaaggaagac
     3421 gcgctaacct cattagatag tgggcttcgc tttactgaga cgaatgatcc aaatattttc
     3481 gtatgtccga gttgtgggaa taatgttgag ttgcctgtag aggatttaat tgtatgagaa
     3541 aaaggttttt attttataat tcttccgatt taattgggca acaagctgtt gcccaaacag
     3601 acatacagaa aagccaacaa gttgttggcc aaatattcca aatcccttgc ggtaataact
     3661 attgtcattc agcacaggct acaatggtaa ttaatttatg aatgattgga aagaattcat
     3721 aattcaaatt ttaattatgg cgaaatcgcc acaattaaaa ggaacgatca tcaatccaga
     3781 gtttaaaccc gtcgaattcg acgggtttag aaaacaggct ggagttgcaa atagtaaaga
     3841 ttaaaaatca tgcaagaacg aaaattaaac cgattatcag gatatgatta ttctacagct
     3901 ggttattatt tcataacgtc atgcgttaaa aatagagaat gtcttttggg agaaattttt
     3961 aattcgaaaa tggatttaaa tgaattcgga aaaattgtaa atacctgttg gttggattta
     4021 ccaaatcatt ataaaaatat ccaattggat gaatttgtta tcatgcctaa tcattttcat
     4081 gggattatat atatcatggg gaacggtgac gttatcgacg tagggaacgg tttcaaaccg
     4141 ttccctacat tggcgatgaa accgttcccc acagggaaaa atcaaggatt gccggaaatc
     4201 gttcgcggat tcaaaacgtt ttcatctcgg cgaatcaacg aaacaaatcc aaaccaaaaa
     4261 ttccaatggc aaaaatccta ccacgaccga atcatcagaa acgaacgaga attattttta
     4321 attcgcaggt atattagaaa taaccccatg aattggatga aggacaaaca ttatatatga
     4381 ataataatat catacagatt gggaacggtg atatcgccgg tgtaaggaac ggtgacatca
     4441 ccgacgtaag gaacggtttg aaaccgttcc ccacgatgac gacattgcca ccgttcccaa
     4501 ttcaaacatc acgtttatca ttatacaaca caaccagcaa tgcggataac ggtaattaat
     4561 ttatgaatga atggaaagaa actgatcttg gaacaattcc gaatcactgg ctagttgtaa
     4621 atgttgatta tttaattgaa cataagataa ttgagaagcc gttagatgga aatcatggtg
     4681 aattgcatcc atcagggaat gattttgttg ataaggggat tccatttgta atggcaacag
     4741 atgtagtaaa tggtaaagtt gatctagcta attgtaaatt tattacagaa gagcaagcgg
     4801 gaaaattaaa taaaggattc tcattacctg gtgatgtttt attaacacac aaagcaagta
     4861 tcgggcgaac ggcaattgtt ggtaaaataa catcaccata tattatgctt actccacaag
     4921 ttacatatta tcgcgttatt aatgaaaata agttgcttaa caaatattta aagtattttt
     4981 ttgattcacc tgtttttcaa gaaatactta ttaatcatag tgatagtgga tctactagag
     5041 catatattgg aataacagca caaagagaat tgcccatcct ccttccccct ctccccgaac
     5101 aacgtgcaat agccgctgtg ctatccagcc ttgatgataa aatcgatcta ttgcaccgcc
     5161 agaacaagac gttagaagca atggtagaga ctttgttcaa tgaacaattt ataatgaata
     5221 atgaagaatg gaaaataggt caagtgtcgg attttattga gtttaatcct acacgatcac
     5281 taccaaaagg aacaattgca ccttatcttg aaatggctaa tgtaaataca tctgtgtttc
     5341 atcctgcagg ctggtatgat agagaattta gttcgggtat gaaatttatt aacggcgata
     5401 cattgttagc ccgaattact ccttgcttag agaatggtaa gtccgcatat attaccttct
     5461 taaatgaaaa ccaagttggt tggggttcta ctgaatttat tgtgatgcgt tcaaaagaaa
     5521 atcttcatac tctatttaca tacgcactgg caagaaacaa agattttcgt gattatgcag
     5581 aaggttgtct tgaagggtca agtggaagac aaagggttaa tatcgatcat ttaataaaat
     5641 ttgaaattaa tgtccctcca aaagaaaata tagaacggtt taattcttta atggaaaata
     5701 ttgaacctaa actgcataat aattttattc aaatccaaac cctaaccaaa ctccgcgata
     5761 cgttattacc caaactgatg agcg
//
LOCUS       DAOQOS010000218         5777 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_8299_length_5777_cov_5.484096, whole genome shotgun sequence.
ACCESSION   DAOQOS010000218 DAOQOS010000000
VERSION     DAOQOS010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5777)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5777)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5777
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8299_length_5777_cov_5.484096"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(197..1450)
                     /locus_tag="PLF66_25325"
     CDS             complement(197..1450)
                     /locus_tag="PLF66_25325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020773638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HMZ67435.1"
                     /translation="MIKILPEISNDFLSDDNFVPHFQPIINTVTRDILGYEVLGRIYS
                     TSHNDFVSLGAFFHGKTTNLSEKVQVDRLIREKAIRYLKSTGTNTKLFFNMMPNMLST
                     IHQEELLDPNRFHMIQLVEKYGINKDNIIIEITEDEFQGKMERLLKMVEIFRNYGFKI
                     AIDDVGVGFSNLERIGYIHPDIIKVDIKIMRESLGSNSFMQVLTALSEMSVKLGSELL
                     FEGIETEQELTLAFKMGASLLQGFYFSKALKTFQSKQSYANELKDALEKFSGIRFMEI
                     VEDFHKLQFIIDTLSSIFEELERKVSSDSTLRPQDVLAHRLKDFPDHIIHVLLTDMNG
                     YQISPSYSRISQNEWEVSEVGILNNYAWKPYFIKHKSETYFFKKKWGVTKPLYDIQTQ
                     SQYVIFTFTISSDTILIARVNWNYE"
     gene            1784..3271
                     /locus_tag="PLF66_25330"
     CDS             1784..3271
                     /locus_tag="PLF66_25330"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HMZ67436.1"
                     /translation="MMTNFEELTQKIIETDEIEMTFRYISDEDLQQIYSLLQRILQKK
                     SKIYLCEYLFMMLKEVLVNSIRASAKREFFARNGLDPEKDDDYKTGIVRFKNEIILNW
                     SAHASYLRYSQFFIVLRFKFSENIDIEIINNSPLVEYEKMRIDSRLSSADKYKNILDA
                     YTDLSDSQESAGLGIVMIVLLLKSIGLDKSYFKIECVDSNTVTSIHIPAQIIPHEIAS
                     EIREKILKEVDMLPSLPKSLQKIMEMCNSENLEFSELALEIEKNPALSADLLKLSNSS
                     SFVTRMPVNTILQAVKIVGSNNILKMLYAVSSYTLLKEKFPRMEKEWEHANKTSIFAV
                     KIAEDFGLIDSIETISLGALLHDIGKILLLAVEGKLFQSIQELTKNLQMESNRALEEA
                     TIGISHSELGILLAKKWNYPEDLSALIEFHQDPFLAPEKYKRIVEIVYLANILSNKLE
                     TDVNFYSYDLAVLHSFNIYSIEDFNSFHYKLESHFRSNNKNPFFG"
     gene            3556..4884
                     /locus_tag="PLF66_25335"
     CDS             3556..4884
                     /locus_tag="PLF66_25335"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HMZ67437.1"
                     /translation="MTFENLDIISSICKALEEEGYKNPTPIQVQSIPVALEGKDILGA
                     AQTGTGKTAAFLVPILQRLQSRPKSNHHTGVQALVLAPTRELAVQIGDSIKVYGKYTS
                     QKYAVIFGGVSQRNQENVLRGKIDIIVATPGRLLDLMNQKIIKLHSVNTLVLDEADKM
                     LSMGFIDDVRKIISKIPVKRQTLFFSATMPKEIQKLADEILKDPVKVEVARVSSTNEN
                     IAQEIFPVDRGNKNSLLIHLLKEESFSKVLVFSRTKHNANKVVKELNRNHIKSDAIHG
                     NKSQSARQKALDHFKSGGLRVLVATDIASRGIDIDDVTHVINYDLPNEPEAYVHRIGR
                     TGRAGSIGKAISFCDRDERNYLRSIERLIKKTIPAVLDHPFPYGTHIPKEENKKPIEH
                     HQPKQVSDKPKKPSEHSHKKNQESNKKKKRKKKSNASNSFHIQKKKKNNS"
     gene            5323..>5777
                     /gene="cas6"
                     /locus_tag="PLF66_25340"
     CDS             5323..>5777
                     /gene="cas6"
                     /locus_tag="PLF66_25340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008398585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-MYXAN CRISPR-associated protein
                     Cas6/Cmx6"
                     /protein_id="HMZ67438.1"
                     /translation="MPYIELKFPVQGKIIPADHGYLLYSAISKLISDNHETENLAICG
                     ISGIPDQKRNLHLDKTSKLRIRIEHENLPIFLKLAGKSLSIGDSKINLAIPNVSILKP
                     RKDLYSRLVTIKGFTEAEPFIEAVKRQLINLEIHKEPILFKNQSTGKYIR"
BASE COUNT         2002 a          884 c          937 g         1954 t
ORIGIN      
        1 cctatttttt tatacaatac aatgtgtttt cgattctttt gtttcattca aaagcagtaa
       61 ctgttcacaa tcaaaatgga ttcatggaat acaaataact gtgcccgtac cgatttcacg
      121 ttaatatagt atttttgtta ggttaaaacc ataattacaa tgattagact tcgtgcaata
      181 gaaaagattt ttagaattac tcgtaattcc aattgactcg tgctatcaaa atcgtatcac
      241 tcgagatagt gaatgtaaag attacatact gtgattgggt ttgtatgtcg tagagtggtt
      301 ttgtaacacc ccattttttc ttgaagaaat atgtttctga tttatgtttt ataaagtaag
      361 gtttccaagc ataattattg agtataccta cttcagaaac ttcccactca ttttgtgaaa
      421 tccgagaata agaaggcgat atttgatatc cattcatatc agttagtaaa acatgaatga
      481 tatggtcagg aaaatctttc agtctatgag ccaaaacatc ctgaggtcga agggttgagt
      541 cagaagaaac ttttctttct aattcttcga aaatagagga aagtgtatca attataaatt
      601 gtaatttatg aaaatcctct acaatttcca taaaccgaat tccactaaat ttctcgagag
      661 catcttttag ttcatttgca taggattgct tagactgaaa tgtctttaac gcttttgaaa
      721 aatagaaacc ttgtagcaag cttgcaccca ttttaaatgc gagtgttaat tcctgttccg
      781 tttcaatacc ttcaaataat aattctgaac ctaatttaac agacatttca gataaagcag
      841 tgaggacttg cataaaggaa ttacttccta aggattctct catgatctta atatctactt
      901 ttataatgtc agggtgaatg tatccaattc tttctaggtt cgaaaagccg actcctacat
      961 catcgatggc aattttgaat ccatagtttc gaaagatttc caccattttt aagagacgtt
     1021 ccatttttcc ctgaaactca tcttctgtaa tttcgatgat gatattatct ttattgattc
     1081 catacttctc cactagttgg atcatatgga atcgatttgg atctaataat tcctcttggt
     1141 ggatagtaga aagcatattt ggcatcatgt taaaaaagag ctttgtgttt gttcctgtgc
     1201 tcttgagata acgaattgct ttttctcgga tgagtctgtc tacttggact ttttctgaga
     1261 ggttagtggt ctttccatga aagaatgcac ctagagaaac aaaatcattg tgactagtcg
     1321 aatagattct acctaatacc tcataaccta aaatatctct tgtaacagta ttaatgattg
     1381 gctgaaaatg aggaacgaaa ttatcatcac ttaaaaagtc attactgatt tctggtagaa
     1441 tttttatcat attctttttc ttctacttat gttgtgatct tgattgttta aaaaagcaac
     1501 taaaatattt ttgaatttta attcaccata attttcaaga ttgcagtttg cagattctta
     1561 cctcatttaa cattctactt acctcaaata aaaaattgct gaataaaatc cattcgctaa
     1621 acaatctttt tctatacatt tattacagtt ttgcagaata tgatataaat tagtttgctc
     1681 agagcaaaca gagtttgaca tatacgactc atttaaacaa agtattttca gaactttgta
     1741 tatctatttt cctttggaag tgaaatagat agattcaaaa actatgatga caaattttga
     1801 ggaattaaca caaaagataa tagaaacgga tgaaatagaa atgacgtttc gctatatttc
     1861 agatgaagac ttacagcaga tttacagttt gctacagaga attcttcaaa aaaaaagtaa
     1921 gatatatcta tgcgaatacc tttttatgat gttaaaggaa gtcttagtaa attcaattcg
     1981 tgcttctgca aagagagaat tttttgcaag aaatggtctt gatccagaaa aagatgatga
     2041 ctataaaact ggtattgttc gtttcaaaaa tgaaattatt ttaaattggt ctgcacatgc
     2101 aagttatttg cgttattcgc aattctttat cgttttacgt tttaagtttt cagagaatat
     2161 agacattgaa attataaata attcaccttt agtggaatat gagaaaatgc gaattgattc
     2221 gcgcttatct tctgcagata aatataaaaa tattttagat gcttacactg atttatcgga
     2281 ttcccaagaa agtgccggtc ttggaattgt tatgatcgtt cttttgctaa aaagtattgg
     2341 tttagataaa tcctatttta agattgaatg tgttgattct aatacagtta ccagtattca
     2401 tattccagcg cagatcattc cacatgaaat agcaagtgag ataagagaga aaattttaaa
     2461 agaagtagat atgcttcctt cacttcctaa gtctttgcaa aaaattatgg aaatgtgtaa
     2521 ctctgaaaat ttagaatttt cagaacttgc tttagagatt gaaaagaatc cagcactcag
     2581 tgctgatcta ttgaagcttt ccaattcttc tagttttgta acccgaatgc ctgttaatac
     2641 aattctgcaa gcggttaaaa ttgtcggttc caataatatt ttaaaaatgc tttatgccgt
     2701 tagttcttat accttattaa aggaaaaatt tcctagaatg gaaaaagagt gggagcatgc
     2761 aaataaaacc agtatattcg ctgttaaaat tgcagaagac tttggattga ttgattctat
     2821 tgaaacaatc agtcttggtg ctcttttgca tgatattgga aagatattgc ttttagctgt
     2881 agaagggaag ttatttcagt cgattcaaga gttaacgaaa aacttacaaa tggaaagcaa
     2941 tagagcctta gaagaggcta ctataggaat tagtcattca gagttaggaa ttcttctcgc
     3001 aaaaaaatgg aattatcctg aagacttatc tgctcttatc gaatttcatc aggatccttt
     3061 tcttgctcca gaaaaatata aacggattgt agagattgta tatcttgcaa acattctttc
     3121 caataaatta gaaacggacg ttaatttcta ttcttatgat ttggctgtat tacatagttt
     3181 taatatctat tcaatagagg attttaattc ctttcattac aagctagaat ctcattttag
     3241 atctaataat aaaaatcctt ttttcggctg agagttttct acttaagagg agagaatatt
     3301 ttttctttaa tcactcttct tacataaaat tataaagtta gaaacgaacc tccctattat
     3361 ttcttttgat ttgatcaagc gtattatcat ttttctagga caatgggaac aaatctagtt
     3421 tgaacttttg gtctaaacaa actctatctt ttattttatt aaggaataaa aatgtttgtt
     3481 gagtgaatca ttttatcttt gcactggtaa aataaataat cctatctttg tggctcttat
     3541 ttgaaaaaat gacgtatgac atttgaaaac ttagatatta ttagttcaat ttgcaaagca
     3601 ttagaagaag aaggatataa aaatccaact ccaattcaag ttcaatccat accagttgcc
     3661 ttagaaggaa aagatatttt aggcgcagca caaacaggaa caggaaaaac tgctgctttt
     3721 ttagttccta tactacaaag attacaatct cgtcctaaat ccaatcatca tactggagtt
     3781 caagcattag tcttagctcc tacccgtgag ttagcggttc aaatagggga tagtataaaa
     3841 gtttatggta aatatacttc tcaaaagtat gcagttattt ttggaggagt ttcccagaga
     3901 aatcaagaaa atgtattaag aggaaaaatc gatataatag tagcaactcc aggaagatta
     3961 ttagatttaa tgaatcaaaa gattatcaag ctacattctg taaatactct tgttctagat
     4021 gaagcagata aaatgctaag tatgggtttt attgatgatg ttcgaaaaat tattagtaaa
     4081 attcccgtga aacgtcagac gctctttttc tctgctacaa tgccaaaaga aattcagaaa
     4141 ctagcggatg aaatattaaa agatcctgta aaagtagaag ttgcgcgggt ttcatctaca
     4201 aatgaaaata ttgcacaaga aatatttcca gtcgataggg gcaataaaaa ctctctctta
     4261 attcaccttt taaaagaaga gtccttttct aaagtcttag ttttttctag aactaaacac
     4321 aatgcaaata aagtagtaaa ggaattaaat cgaaatcata ttaagtccga tgcaattcat
     4381 ggtaataaat ctcagagtgc aagacaaaaa gctttagatc attttaaatc tggtggatta
     4441 cgcgtattag tcgcaactga tattgcttcc cgtggaatcg atatagatga tgttactcat
     4501 gtcattaatt atgatttgcc gaatgaacca gaagcttatg ttcatagaat tggtcgaact
     4561 ggaagagcgg gctctattgg gaaagcaatt tctttttgtg atcgagatga aagaaactat
     4621 ttaagatcga ttgagagact cattaaaaaa acgattcctg ctgttcttga tcatcctttc
     4681 ccgtatggaa ctcatattcc aaaagaggag aataaaaaac caatcgagca tcaccaacct
     4741 aaacaagtct ctgataagcc taaaaagcct tcagaacact ctcataagaa aaatcaagaa
     4801 tctaataaga aaaagaaacg aaagaaaaag tctaacgcaa gtaattcgtt tcacattcag
     4861 aaaaagaaga aaaacaattc ctaatcttct ggattacata tccccaaagc taccctaaac
     4921 ttagaggtaa agattgggta aacttatcct aaatatccct tgctacctat ctttagggga
     4981 agtttagtcc tatgtttagg ggatgtttgg tgtattgtta tccaaattga aattgtctgc
     5041 taaatcgaat tcacgttaag aaaattctaa tcttttcgat agtgagagat tcttatttaa
     5101 gggaaccaaa caaatagctt ccagtgagtg gtaaatttga aattgtttct attattggtt
     5161 cctaagaagt tctagatata aattatctgt tttccctata aaaaatcttg actgaaaata
     5221 ataaaatttt taaattttat ctagggtatg ttctaatttg tcctatcctt ttatgaaaat
     5281 aatattttag aaagactcat tggctttaag ggagaatcgt ttatgcctta tattgaatta
     5341 aaatttccag tgcaaggaaa gataattcca gcagatcatg gttacttact ttattctgct
     5401 atatccaaat taatatcaga taatcatgaa acagaaaatc ttgcgatttg tggcattagt
     5461 gggataccag atcaaaagag aaatttacat ttagataaga ctagcaaatt aagaatccga
     5521 atcgagcatg aaaatcttcc catcttttta aaacttgctg gaaaatcttt atcgataggc
     5581 gattcgaaaa ttaatttagc cattccaaac gtatccatat taaaaccaag aaaagattta
     5641 tatagcagac tcgttactat caaaggtttt actgaagctg agccttttat agaggccgtt
     5701 aagcggcagc ttataaattt agaaattcat aaagagccta tcttatttaa aaatcaatct
     5761 actggaaaat atatacg
//
LOCUS       DAOQOS010000219         5747 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_8374_length_5747_cov_5.917252, whole genome shotgun sequence.
ACCESSION   DAOQOS010000219 DAOQOS010000000
VERSION     DAOQOS010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5747)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5747)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5747
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8374_length_5747_cov_5.917252"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..508
                     /locus_tag="PLF66_25345"
     CDS             <1..508
                     /locus_tag="PLF66_25345"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HMZ67439.1"
                     /translation="QTNILALNAAVEAARAGQHGKGFNVVAEEVRNLASRSANAAKET
                     TKMIEGSIKTVRAGMEIANRTATELGKMTNGVVEVTELVKDIASASIEQSSSVVQTTS
                     GINQISQVTMSAAATAEESSAASLELSSQAEAFQTMVKRFSIKKSKDDKKREQQSSSS
                     GKKKIDLK"
     gene            517..1002
                     /locus_tag="PLF66_25350"
     CDS             517..1002
                     /locus_tag="PLF66_25350"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HMZ67440.1"
                     /translation="MSNTATEIDLTEESGDTMENLYIIFFIDGREYGIEIRYLIEIIA
                     FQPITYVPNLPNYVKGVINLRGKIIPVIDVRLRFGLPAIEYTEFTCILIMSFNNLSAG
                     LIVDGVKEVLKIPSDSIEPSPGFEEADMERFIDSIGKAGDTLKILINLNKFIHEKDVK
                     I"
     gene            1015..2844
                     /locus_tag="PLF66_25355"
     CDS             1015..2844
                     /locus_tag="PLF66_25355"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HMZ67441.1"
                     /translation="MLGENELSDLVLDSQEILDKAEGILIQLEKDLNTSSVDPDKVNS
                     LFRYFHTLKGNAGFFNFNVIVTLAHSAESLLEIVRNDNSHLNQKMIQLFMETRDTLYE
                     IFSNLNEKKTDESYKEKADILIPKLFYIKENLKPENTQPKEDEPRFGIFKKKKIDLNI
                     PTPSLEEVAVNDNLPEKPKESQQNPEVKTNPSKEPNGNGLNLTASAVKKDIRIETDKL
                     DYLLDMIGELVIAESLVTQNPDIKDLELPNFSIQSSYLRKVVRNLQEISLQLRMITLE
                     GLFNKMERLVRDLAQKTGKKVSLQVTGKETEIDKNALEILTDPLVHIIRNCIDHGLET
                     GQERLAKGKLESGNIRIEAIHAGNEVWIIISDDGAGLDKDKILNKAKNRGLIREGEEE
                     NLKEEKIWDFIFQPGFSTSDKVTDISGRGVGLDIVRRNIAKINGEVEVKTQKDKGASF
                     ILKLPLTIAIIEGMVVTYNGVFYIIPTIDVKETLNLAESNIDYVDQDKRVIRFRDHLI
                     PVVSVEKLLNPELEPNNASYDKCLYVIIVEYKNSSLALVFDGIIGNQSVVIKPIPDYL
                     KGTKGFSGCTILGSGNIGMILDIRFFINNYYRENKESEIQHGQ"
     gene            2834..3913
                     /locus_tag="PLF66_25360"
     CDS             2834..3913
                     /locus_tag="PLF66_25360"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: protein motif:HMM:NF001965.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HMZ67442.1"
                     /translation="MDNNRKIKVFVVDDQRVIRNVIKKMLATDPQLEVVGEASNPFEA
                     SELIPEVEPDVLTLDVEMPRMDGVEFLSKLMPQYPIPVIMVSSTTEEGSERAIQAMQN
                     GAIDFVTKPDGGAITLENTQDELIQKIKAAAFVDMNRWLTHLKYKALTATPPPSNAKR
                     PKSKYNLIAIGASTGGVTAIRQVLSTVPKDFPCIVITQHMPPGFTATFAKGVSKDYGL
                     DVREAKDQDPIQPGRIYVAPGDYHLVVNKRNGINHTVGLTRSDKVNGHRPSVDVLFYS
                     IVENKLAPETIGVILTGMGADGAKGLLAMRDAGSRTLGESEHSCLIYGMPKAAYDIGA
                     VEEQLALSQMVPKLKTYLNYTGSYV"
     gene            3906..5153
                     /locus_tag="PLF66_25365"
     CDS             3906..5153
                     /locus_tag="PLF66_25365"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HMZ67443.1"
                     /translation="MSDKLILYSKNKALIADVKKFISESLYILTVTDNVDKLRSHLGE
                     SETSLLLYDFLDSSKENQNDLADLLSDFPFQLVILIVDRENSDEFLTMVENYHVYDLI
                     GYPIYKIKLLFTLSKAMTYGRVMSKISDFKSNEKQVFESMIKVFDWRKELDTRKVESL
                     ANELIHQINISFFHGSGLGSLISTLSIMFAKAKLDEEKKMYLVHEKVYHLLKDNYEEI
                     LKLSDSLTKTQSILSEKNYYTKEVVLKDFYTTLVSIVKDMKNVAAIKKQKIVMGDFPR
                     DLVTHKIIADLPNVKLVFEELITNAIKYSEDGDTIYILFSHDKSDIGIRILNPAYKNS
                     DGTIGITESNAHKIFEPFFRLTNVVDDRFMQETFRFGLGLTVVNKIIDLHRGNIAFYT
                     TDNNMRKEGNMKDVCVWIRFPII"
     gene            5216..5581
                     /locus_tag="PLF66_25370"
     CDS             5216..5581
                     /locus_tag="PLF66_25370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008595906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HMZ67444.1"
                     /translation="MKKILIVDDSPMVLNIIETALSLEDYDIVRASNADEALKKVEAE
                     KFDFGIFDVNMPGKTGIELIPLVLNHPNGKDMKIVILTTESSAEIRKKGEDAGASAWM
                     VKPFKNAEMLELLKQLGDA"
BASE COUNT         2087 a          828 c         1192 g         1640 t
ORIGIN      
        1 ccaaaccaat atacttgcgt taaacgcagc ggtagaagca gcgcgagcag gacagcatgg
       61 caagggcttt aacgtagtag cagaagaagt gagaaaccta gcatctagaa gtgcgaatgc
      121 agcgaaagag acaacgaaga tgatagaagg atcgatcaag acagttcgag ctgggatgga
      181 gatagcgaat cgaacagcaa ccgagctagg caagatgacg aacggagtag tggaagtaac
      241 cgaactagtg aaggatattg caagtgcatc gatagaacag tcttcttctg tagtgcagac
      301 gacgagtgga atcaaccaga tatcgcaagt gacgatgagt gcagcagcga cagcggaaga
      361 gagttcagct gcaagtcttg agttatcaag tcaagcggaa gccttccaga cgatggtgaa
      421 gcgattttcg attaagaagt cgaaagatga taagaagaga gagcaacaat catcgtcctc
      481 agggaagaag aagattgact tgaaatagga gggtctatgt caaatacagc aacggaaata
      541 gatctaacgg aggaatcggg tgatacgatg gagaatctgt atatcatctt tttcatagat
      601 ggaagagaat atggaataga gatacgatat ctgatagaga tcatagcgtt tcagccgata
      661 acatatgttc cgaatctacc gaattatgtg aagggagtaa tcaatctgag aggaaagata
      721 attccagtga tagatgttcg gttgcgattt ggattgcctg cgatagagta tacagagttt
      781 acgtgtatat taatcatgag ttttaacaat ttaagtgcag ggttgatagt agatggagtg
      841 aaggaagtat tgaaaattcc atctgattcg atagagccct cacccggatt tgaggaagcg
      901 gacatggaaa gatttatcga ttcgattgga aaggctggag atacattaaa aattctaatc
      961 aatttgaata agtttatcca tgagaaagat gtaaagatat agcatagggg taaaatgtta
     1021 ggggaaaacg aactctcaga tctcgttctc gattcacagg aaattctaga taaagcagaa
     1081 ggaattctga ttcagctaga aaaagattta aacacatctt ctgtggatcc agataaagta
     1141 aattccttat ttaggtattt tcatacactc aaaggaaatg ccggattctt taattttaac
     1201 gtgatcgtaa cgttagctca ttcagctgag tcacttttgg aaattgtgag aaatgataac
     1261 tcacatttaa atcaaaagat gattcagttg tttatggaga cacgagacac cctttatgaa
     1321 atattctcga atctaaatga aaagaaaaca gatgaaagtt ataaagaaaa agcagatatt
     1381 ttaattccaa agctttttta tataaaagaa aatttaaaac cggaaaatac gcaaccgaaa
     1441 gaagatgaac ctcggtttgg aatatttaaa aagaaaaaaa tagatttaaa tattccaacg
     1501 ccatctcttg aggaagtggc ggttaatgat aatttgccgg agaaaccaaa agaaagtcag
     1561 caaaatccgg aagttaaaac gaatccatcc aaagaaccaa atgggaatgg tctaaatcta
     1621 acggcttctg ccgttaagaa agacattcga attgaaacag ataaattaga ttatttatta
     1681 gatatgattg gggagttggt aattgcagaa tccctagtga ctcaaaatcc agatattaaa
     1741 gatttagagc tacctaattt ttcaattcaa tcctcttact taagaaaagt tgttcgaaat
     1801 ttacaagaaa tatctttgca gctaagaatg attaccctcg aaggtctttt taataaaatg
     1861 gaaagacttg tcagagattt agcgcaaaag actgggaaga aagttagttt acaagtgaca
     1921 ggtaaagaaa cagagataga taaaaatgcg ttagagatac taacagatcc tcttgtgcat
     1981 attattagaa actgtattga tcatggatta gaaacaggac aagaaagatt agcaaaaggc
     2041 aaattagaat caggcaatat aaggattgaa gcgattcatg ccggaaatga agtttggatc
     2101 attatttcag atgacggtgc tggacttgat aaagataaga ttttaaataa agcaaaaaat
     2161 cgtggcttaa ttcgcgaagg cgaagaggaa aatttaaaag aagaaaagat ctgggatttt
     2221 atttttcaac caggattttc cacaagtgat aaagtgactg acatatcagg tcgtggcgtt
     2281 ggtctagata tagttcgccg aaatattgct aagataaatg gagaagtaga agtcaaaact
     2341 caaaaagata aaggggcttc ctttattttg aaattacctc ttacgatcgc cattattgag
     2401 ggaatggtgg taacatataa tggagtattc tacattattc caacaatcga tgtgaaagag
     2461 accttgaatc tagctgagtc aaatattgat tatgttgacc aagacaaacg agtgattcga
     2521 tttagagatc atttgattcc ggttgtttcc gttgagaaat tattaaatcc agaattagag
     2581 ccaaataatg cttcttatga taaatgcctt tatgttatta ttgtagaata taaaaatagt
     2641 tcgcttgctc ttgtgtttga tgggattatt ggaaatcaat ctgtagtgat taagccaatc
     2701 ccagactatc tcaaaggaac aaaagggttt tccggttgca cgattttagg aagtgggaat
     2761 attgggatga tattagatat tagattcttt attaataatt attacagaga gaacaaagaa
     2821 tcagaaattc aacatggaca ataaccgtaa gataaaagta ttcgttgttg atgatcaaag
     2881 ggtaattcga aatgtaatca agaagatgct tgccacagat cctcaattag aggtggttgg
     2941 tgaagcatcc aatccatttg aagcaagtga acttattcca gaagtagaac cagatgtttt
     3001 gacactagat gtagaaatgc ctagaatgga cggagtagag tttttaagta aactgatgcc
     3061 tcaatatcca attcctgtga tcatggtaag tagcacaaca gaagaaggct ccgagagagc
     3121 aattcaagct atgcaaaatg gagcaattga ttttgttacg aagccagacg gcggagcgat
     3181 cactttagaa aatacacaag atgaattaat tcaaaaaatc aaagcagctg cttttgtaga
     3241 tatgaatcga tggttgactc acctaaaata caaagcgttg acagcaactc caccaccttc
     3301 caatgcaaag agacccaaga gtaaatacaa tctaatcgcg attggcgcat cgactggtgg
     3361 agtgactgct atacgacaag ttctatcaac tgttccaaag gattttccat gtattgtaat
     3421 tacgcagcac atgcctcctg gattcacggc aacttttgcg aaaggggttt ctaaagatta
     3481 tggattggat gtgagagagg caaaagacca agatccaatt caacctggaa gaatctatgt
     3541 agctccaggc gattatcatt tagtagtaaa caaaagaaat ggtatcaatc atacagttgg
     3601 tctcacacga tctgataaag taaatggtca tcgtccctct gtggatgttc tattttattc
     3661 cattgtagag aataaacttg cacctgagac aatcggtgtt atactgacgg gaatgggagc
     3721 agatggggca aaaggactac ttgcaatgag agatgcaggt tcaaggacac ttggtgagtc
     3781 agagcattct tgtttgatat atggaatgcc gaaagctgcc tatgatatag gtgctgtaga
     3841 agagcaatta gctctttctc agatggttcc caaattaaaa acatatttga attatactgg
     3901 atcctatgtc tgataaactg atactctatt caaagaataa agcgttaatc gcagatgtaa
     3961 agaaatttat aagtgaatca ttgtatattc ttactgtgac agataacgtt gataaattga
     4021 gaagtcattt aggagaaagt gaaacatctc tacttttata tgacttttta gattcatcca
     4081 aagaaaatca gaatgactta gccgacttac tttcggattt tccatttcaa ttggtaattt
     4141 taattgtaga tcgagagaat agtgatgagt ttttaactat ggtagaaaat taccatgttt
     4201 atgatttaat tgggtatcca atttataaaa taaaactact ctttactctt tccaaagcaa
     4261 tgacttatgg aagagtcatg tcaaagattt cagacttcaa atcgaatgaa aaacaagtct
     4321 tcgagtccat gataaaagtt ttcgattggc gaaaggaatt agacactagg aaagttgagt
     4381 ctttagcaaa tgaattaatt catcaaatca atataagctt ttttcacggt agtggattgg
     4441 gatctttgat aagcactctc agtattatgt ttgcaaaagc caagttagat gaagaaaaaa
     4501 aaatgtatct cgttcatgag aaagtctatc atctattaaa agataattac gaagaaatat
     4561 taaaattaag cgatagttta accaagactc aatctatttt atccgaaaag aattattata
     4621 caaaagaagt agtattaaaa gatttttata ctacattagt aagcattgta aaggacatga
     4681 aaaatgtcgc agctatcaaa aaacaaaaaa tagtaatggg tgattttcca agagatttag
     4741 tcacacataa aataatagca gatcttccaa atgtcaaatt agtatttgaa gaattaatta
     4801 caaatgctat caaatattca gaagatggtg acacaatcta cattcttttt tcgcatgata
     4861 aatctgatat tgggattcga atcttaaatc ctgcctacaa aaattcagat ggaactatcg
     4921 gtattactga gagtaatgca cataaaatct ttgagccttt ttttagattg acaaacgttg
     4981 ttgacgatag atttatgcag gagacatttc gcttcggttt gggactaacg gttgttaata
     5041 agattattga cttgcacaga ggaaatattg cattttatac aacagataat aatatgcgaa
     5101 aagaaggaaa tatgaaagat gtttgtgtct ggattcggtt tccaattata taaatatttt
     5161 ttctgaaatg gaactaatag aaaaaagtta aaatgttatt taataaggat tgtttatgaa
     5221 gaaaatactc attgtcgatg attctccaat ggttttaaac attattgaaa ctgcacttag
     5281 tttagaggac tatgatatcg ttcgagcttc gaatgcggat gaagcattaa aaaaagtgga
     5341 agctgagaaa tttgattttg gaattttcga tgtaaacatg ccaggtaaga ctggaattga
     5401 attgatccca ttggtcttaa atcatcccaa tggaaaagat atgaaaatag tcattttaac
     5461 aactgaatcg agtgctgaaa ttagaaagaa aggggaagat gcaggagcat ctgcttggat
     5521 ggtgaaacca tttaaaaatg cagaaatgct agaattgtta aaacaactgg gagatgctta
     5581 gaatagaaat actatcttta tctttaaaat cttttttata cttgcttgtt ctctgaaatt
     5641 aattatgctt tcctcattaa caaatcgagg agagttttat ggcagcagaa gaaagacaaa
     5701 gagaacgtga tttaatttta tccccaaatg aatttgcttt tatttcc
//
LOCUS       DAOQOS010000220         5716 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_8458_length_5716_cov_6.760290, whole genome shotgun sequence.
ACCESSION   DAOQOS010000220 DAOQOS010000000
VERSION     DAOQOS010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5716)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5716)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5716
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8458_length_5716_cov_6.760290"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(168..3971)
                     /locus_tag="PLF66_25375"
     CDS             complement(168..3971)
                     /locus_tag="PLF66_25375"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HMZ67445.1"
                     /translation="MANYPVEILNKIINILKEDRYKDKSKKSVDFPSYARLKDFERYS
                     KGDEDTLVTPIILEILKAIGYKSGINIFQQEFKVGDKPDFRTKTNKFILDAKSTGVDI
                     STYRTGGIESATQQIARYLKNLPGYDYGILFNLEKFEFFQRTFDESNSVKARRLEDRT
                     INLVKLYQDYHKGQTEIDKSEDFKNLKWFIENFSFKEISSDEYIEIIKSREKSKLNPP
                     DKEFLQKTVYKLLDSIQDDIQEQVSDYEEGSDDWSKIQIELRKIKNEIKLPDSENQTK
                     ILIQEFVKQASYVLLTKLILIRILEDNDLIEKNLYNGGFKKLTEPPFNYTLFKILDQA
                     KLEGKHFYPYFFATTAYDFDPSKNDIFLHALYELSNFNFAQVNFDLIGDLYEHYLNLD
                     DRKEKGQYYTPHYIVEFILNRVGFTGKYKGDIDSRTLWDPASGSGGFIVEAARRLRVA
                     GINNPSDAKKSIVNNIYATELTAFASFLCELNTVIQILPLVKSLEARKNQKIDVLRIF
                     KKDSLLQAFNHEEIGIKETAYPEVHILDSSKEMKFHILQNKNDFDFVVGNPPYVGESG
                     HKELFRPLQDHPYWKKHYLGKSDYLYYFIILGLNKLKDGGKLAFITTQYWLTADGAKN
                     LRKYILETSKIVEIIDFKGIKLFPEAKGQENIVFILERCDEESERINNKIKLIHFKKE
                     WVLDDKEYITSENASTTNFENWKKLITNPSQFEIFSNPTKINFNLGKFKREDIADVYY
                     SAVLQGELDSEAWYIFKKERDEFSTQDKNIIPLEKLFNVKQGVVPGPIDTKKIIKKLS
                     PSTIHTFNISDNEGVFVLTNNELEEKKIEKTIVYPFNKNSDIKKSYINFSTNDFLVYT
                     PKIDILERFPNFKNHIERYYEYLITRREVEQEKIRWFDLWWPREETLFESEKIMISYR
                     TNTNSFAYTNYPFYSATDTYYISKKPETKESLKYVFAVLVSTKILDWLKNGNCKIKGN
                     ALELFTTSVSKIPIRRIDFAKPKDVAIHDLLAGDYSVEEKKGYKYNSTSNTWIKEKGI
                     VDYYMDLIKELYGMVDDGFIFDPTQPLKQPKDISIAISKLSGYLKKTDFSSEIIDDLS
                     FFESNTITNPVNADERKEAEAIFKSRNYYFKIKNKNFEIKKIGEIIGSGYTLYEHDSE
                     LKKYNYKHQLELITKTNEKIYVEIKGKENLENLILMIESLFKEKKIIIWDDLRSLPMI
                     NEKLNKFIETKKDFIYQSLKPVDKSAKLLIKEIVKAHSVVATQMIQNVFFMQELIDAL
                     VEEIY"
     gene            complement(4377..5648)
                     /locus_tag="PLF66_25380"
     CDS             complement(4377..5648)
                     /locus_tag="PLF66_25380"
                     /inference="COORDINATES: protein motif:HMM:NF016994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythromycin esterase family protein"
                     /protein_id="HMZ67446.1"
                     /translation="MENGKTLSISDWIKSNFIPFHSISSEDSFYDLEPLRDIIGNAKV
                     VALGENSHFIKEFCQIRFRILRFLVEKCGFNVHALEFGFTEGFEVNNWIHNHERKEEE
                     LIDLLGHFPYPPEIHDTIKWIRNYNKSKSKPLDFVGIDVPRNGGSFFPSLEVINDFFK
                     VADPDGLVFISDIWDITKKIDGFSTAQSAFMLNKISDTEKDKITSRINRLLYRLEALA
                     PQHLSRFGEKKYNTIYQHLKSICYLDYNSHAMNGFISGKGLPGDMGARDKFMADSILW
                     HLNNSSSKIVLIAHNAHIQKQPIQYGDFLSCLPMGQRLSSSLGSDYISFGLTSVSGHT
                     AALFPDENSKFGFRIENTELSMPVSGSIEKILEEAQVENGIISFKQIPRGSNDPTLIR
                     FDSEYIETSVIDAFDGLIQVPTSSIADGIAY"
BASE COUNT         1768 a          975 c          805 g         2168 t
ORIGIN      
        1 tactccgtta cccaatacga ttctttttat atgttgggtt tgcgaagttg cttgttagaa
       61 ctagtcttta gcaatttccg caaatccaac gttatgcgcg tatactcaac actattcact
      121 aattgtaata tcaatcagga acgctttagt atctgattcc ttgttagtca atatatttct
      181 tcaacaaggg catctattaa ctcttgcata aaaaaaacat tctgaatcat ttgtgttgct
      241 actactgaat gagctttaac aatttcctta attaaaagtt ttgccgactt atccacaggt
      301 tttaatgatt gatatataaa atcctttttc gtttctataa atttatttag tttctcatta
      361 atcatgggaa gacttctcaa atcatcccag attataattt tcttttcttt aaataaactt
      421 tctatcatta gtattaaatt ttctaagttt tcttttccct ttatttctac atatattttc
      481 tcattcgttt ttgtaatcaa ctctagctga tgtttataat tatatttttt taattccgaa
      541 tcatgttcat atagggtata gcctgatccg atgatttcac ctattttttt tatttcaaaa
      601 tttttgtttt taattttaaa ataatagttt ctagatttaa atatagcctc agcttctttt
      661 ctttcatcag cattgactgg gttggtaata gtattcgatt caaagaaaga tagatcatct
      721 attatctcag aagaaaaatc agtcttcttt aaatatcctg atagttttga aatcgcaata
      781 cttatatctt ttggttgctt tagcggttgt gttggatcaa aaataaaacc atcgtcaacc
      841 ataccataga gttcttttat taaatccatg taatagtcaa ctattccttt ctccttaatc
      901 caagtgtttg aagttgaatt gtatttatag cctttctttt cttctacgga atagtctcct
      961 gcaagcaaat catgaatagc cacatcttta ggtttagcaa agtcaattct tctaattgga
     1021 atttttgaaa cggaggttgt aaataattct agagcgttgc ctttaatttt acaatttcca
     1081 ttttttagcc agtctaaaat ttttgtagat acaagtacag caaaaacata ttttaaactt
     1141 tccttcgtct ctggtttttt actaatataa taggtatcgg tagcgctata aaacggataa
     1201 ttggtataag caaaactgtt tgtatttgtc ctataagaaa tcattatttt ttcagattca
     1261 aacaaagtct cttctcttgg ccaccacaaa tcaaaccatc taattttttc ttgttcgact
     1321 tctctcctcg taattaaata ttcatagtat ctttcgatat gattcttaaa attcggaaat
     1381 ctttctagaa tatcaatttt gggggtataa accaaaaaat catttgtgga aaaattgatg
     1441 taagactttt ttatatcaga gtttttgtta aaaggataca caatagtttt ttcaattttt
     1501 ttttcttcta gttcattatt agttagaacg aatactcctt cattatctga gatattaaaa
     1561 gtgtgaattg tgctaggtga taatttctta ataatttttt tagtatctat cggacccgga
     1621 actactcctt gttttacatt aaataatttt tccaaaggaa taatattttt atcctgtgtt
     1681 gagaattcat ctctttcttt tttgaatata taccaagctt ccgaatctaa ttcaccctgt
     1741 aatactgcgc tataatatac atctgcaata tcttctcttt taaatttccc taaattaaaa
     1801 tttattttgg ttggattgga aaatatttcg aattgagagg gattcgtaat caattttttc
     1861 caattttcaa aattagtagt cgaggcattt tcagaagtga tatactcttt atcatctaga
     1921 acccattctt ttttaaaatg aattaacttg attttattat tgattcgttc ggactcttcg
     1981 tcacatcttt ccaaaataaa aacgatattt tcctgtcctt ttgcctccgg aaaaagtttg
     2041 atccccttaa aatcaatgat ttcaactatc ttagatgttt ctaatatata cttacgaaga
     2101 ttctttgcgc catctgctgt tagccagtat tgagttgtga tgaatgctaa tttgccgcca
     2161 tcttttaatt tatttaaacc aagtataata aaataataca aatagtcgct ttttcctagg
     2221 taatgctttt tccagtaggg atggtcttgt agtgggcgaa ataattcttt gtgtccgctt
     2281 tctcccacat aaggaggatt acccacaaca aaatcaaagt catttttatt ctgtaaaata
     2341 tggaatttca tttctttaga agaatctaaa atgtgaactt cgggataagc tgtttctttg
     2401 attccaattt cttcgtgatt aaatgcttga agtaaactgt cttttttaaa aattctaagt
     2461 acatctattt tttgattttt tctagcttct agtgatttta caagaggtaa aatttgaatt
     2521 actgtattta actcgcaaag gaagctagca aatgctgtta actctgtcgc atatatatta
     2581 tttacaatgg atttttttgc atcgctcgga ttgttaatac ctgcaactct caatcgtcta
     2641 gcagcctcta caataaatcc acctgaaccg gaagctggat cccaaagtgt gcgactatca
     2701 atgtctcctt tatattttcc ggtaaatcca actcgattta aaataaattc aacaatataa
     2761 tgcggtgtat agtattgccc tttttctttt ctatcatcta gatttaaata atgttcatat
     2821 aaatctccaa ttaaatcaaa attcacttgg gcgaaattaa aattagaaag ctcatacaaa
     2881 gcatggagga aaatatcatt tttacttgga tcaaaatcat aagcggtggt tgcaaaaaaa
     2941 taaggataaa aatgtttgcc ctctaacttt gcttgatcta aaattttaaa taaagtataa
     3001 ttaaaaggag gctctgtaag ttttttgaat ccgccattgt ataaattctt ttcaattaaa
     3061 tcattatctt ctaatattct aattaaaatt aatttggtaa gtaggacata ggaagcctgt
     3121 tttacaaatt cctgaatcag tatttttgtt tggttttcag aatctggtaa tttaatttca
     3181 ttcttaattt ttcttaattc aatttgtatc ttagaccaat cgtcactacc ctcttcataa
     3241 tcagaaactt gctcttgtat atcatcctga atgctatcca aaagcttata gacagttttt
     3301 tgcagaaact ctttatccgg tgggtttaat ttagattttt ccctactttt aattatttca
     3361 atatattcat cagaactaat ttctttaaat gaaaaatttt ctatgaacca cttcaagttc
     3421 ttaaaatctt ccgacttatc tatctctgtc tgacctttat gataatcttg atataattta
     3481 acaagattga ttgttctatc ttctaacctt cgagctttta cggaattgga ttcatcaaat
     3541 gttctttgaa agaattcaaa tttctctaag ttaaatagaa ttccataatc ataccctggt
     3601 aaatttttta aatatctagc aatctgctga gttgcggatt ctattccacc tgttcgataa
     3661 gtagatatat caactcctgt tgatttagca tctagtataa atttgtttgt tttagttcta
     3721 aaatccggct tatccccaac cttaaattct tgctgaaaaa tatttattcc agatttatat
     3781 ccgatcgctt ttaatatttc aagtatgatg ggagtaacta aggtatcttc atctcctttg
     3841 ctgtatcgtt caaaatcttt taatcgcgca taggaaggaa aatctacact ttttttactc
     3901 ttatctttat accgatcttc ttttaaaata tttataattt tatttagtat ttcaactgga
     3961 taattagcca taattacatc tgattagagg tttaccctag gtcaaatata aatgacctta
     4021 tccctgtttc ttttctattg aattttgctt aatcacattt atctttttgt gaatttctat
     4081 attacttaat ccagaaatag cccaaaatcc aactttgcat ctttccgaga cacgaggtcg
     4141 aggaaaccta tacctgaaag attccacgtc agtccctaga aaaatatggt caaaaataaa
     4201 atggcgtaga acgtacaaga gtagctgacg ttttggtgaa ggcgtgaact ttgctttttt
     4261 ttgcaaagtt catgactgaa ccaaaatgtg gtgaaaccca agccaccgaa tcttctctcg
     4321 gtggcgcagc agctactctt tgttaggcgg tgttttgctt ttttgataat taaatactaa
     4381 tatgctatac catctgcaat actagatgtg ggtacttgaa tcaatccatc aaatgcatct
     4441 attaccgaag tctctatata ttcagaatca aatctaataa gagttggatc atttgagcct
     4501 ctgggtattt gtttaaaact aatgattccg ttttctactt gagcttcttc taaaattttt
     4561 tcgatactgc cggagacagg catacttaat tcagtgtttt ctatacgaaa accaaactta
     4621 gaattctcat ccgggaagag agcagccgta tgaccgctta ctgatgtaag accgaaagaa
     4681 atataatcac ttcctaatga actcgaaaga cgctgaccca ttggtaagca gcttaaaaaa
     4741 tctccatact gaatgggttg tttttgaata tgagcattat gtgctattag aacaattttg
     4801 gaagatgaat tatttagatg ccaaagaatt gaatcagcca taaacttatc tctcgcaccc
     4861 atatctccag gaagtccttt accagaaata aatccgttca tagcatgaga gttatagtct
     4921 aagtagcaga tacttttcaa atgttgatat attgtattat atttcttttc gccgaaacga
     4981 gataaatgtt gtggtgcaag tgcttcgagt ctatataaaa gacgatttat tcgagaagtt
     5041 attttgtcct tctctgtatc tgatatttta tttagcataa atgcagattg ggcagttgaa
     5101 aatccgtcaa tttttttggt gatgtcccaa atgtcggaga taaaaactaa gccgtccgga
     5161 tctgcgactt taaaaaaatc atttatgact tctaaagaag gaaaaaaaga gcctccgttt
     5221 cggggcacat ctatacctac aaaatctaat ggtttacttt ttgatttgtt ataatttctt
     5281 atccatttga ttgtatcgtg aatttcagga ggatatggga aatgccctag taaatctatt
     5341 aactcctctt cttttctttc atgattatga atccaattat ttacttcaaa cccttcagta
     5401 aatccgaatt ctaaagcgtg aacattaaag ccgcattttt ctacaagaaa tctcaaaatt
     5461 cgaaaacgaa tctgacaaaa ttcttttata aaatgagaat tctctcccag tgcaacaact
     5521 tttgcatttc caattatatc tctcaacggc tctaggtcat agaacgaatc ttccgaacta
     5581 atagagtgaa atggaataaa attacttttt atccaatctg aaatactgag tgttttccca
     5641 ttttccatga aatattattt ctcctattta ttaaaaggtt gattatcttt ggcaaagtaa
     5701 aataccgcct aacgtt
//
LOCUS       DAOQOS010000221         5658 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_8608_length_5658_cov_5.738533, whole genome shotgun sequence.
ACCESSION   DAOQOS010000221 DAOQOS010000000
VERSION     DAOQOS010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5658)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5658)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5658
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8608_length_5658_cov_5.738533"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(10..333)
                     /locus_tag="PLF66_25385"
     CDS             complement(10..333)
                     /locus_tag="PLF66_25385"
                     /inference="COORDINATES: protein motif:HMM:NF013633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HMZ67447.1"
                     /translation="MNKNPSFMLEKESTRLNKYISESGLCSRREADEYIQLGTVFING
                     RKASVGDRVFTGDKVVVNGYTIEAKQRKTTIILAYNKPVGVVSTTDPEEKENLLKHRK
                     EKKNQ"
     gene            526..1308
                     /locus_tag="PLF66_25390"
     CDS             526..1308
                     /locus_tag="PLF66_25390"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HMZ67448.1"
                     /translation="MSFLRDRIILITGATGGLGKEMVKQFLPEGSKLILTDLNQASLD
                     ELSSKLDKKGQILFTFTSDLSTQEGCQKVYDTIKSKNYEIDILINNAGVASIGNFMTT
                     PQEEWERQYAINLLAPIRLTKLFLTDMQKRNSGHLVNIASVASFVSAPGLATYSSTKH
                     ALRAFGESLSEELKPTKIRVTNLYPFFTKTPMMESPQYGFKNKKEIPSLLMSTPEEVI
                     RDMINGIKRDELHILPGPVAKATEFLNRIAPQGLSLFFDFLR"
     gene            1457..2911
                     /locus_tag="PLF66_25395"
     CDS             1457..2911
                     /locus_tag="PLF66_25395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017214101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GNAT family
                     N-acetyltransferase/carbon-nitrogen hydrolase family
                     protein"
                     /protein_id="HMZ67449.1"
                     /translation="MDMVYSNAGGAWKQEEFLSQLEHFPEGQFCIEDNGKLVAAVVSM
                     IVKYSNFGDRHTYDQITTEGYLRNHDPEGDTLYGVDIFVHPAYQGLRLGRRLYDARKQ
                     LCQNLNLKRIIIGGRIPKYKDHFTEMSPQEYVTKVKEKEIFDPVLSFQLANGFHVRRI
                     MTNYFPEDKDSKTYATLLEWLNIYYEEKEQLIGGKKPIVRVGVVQWQMRSVDSFEAFL
                     KQIEFFVDAVAGYKADFVLFPEFFNVPLMAKYNKLNPADAIRSLADYTEKIRTEMLHM
                     AVSYNINIISGSMPVYTDEVLYNVSYLLRRDGTWDEQYKIHVTPDEENYWGVKGGTKV
                     KIFDTDVGKIGILICFDVEFPELPRILAEQDMDILFVPFSTDTKNAYLRVRHCAQARA
                     IENECYVVISGSVGNLPDVENMDIQYAQSAVFTPSDFAFSHDAIAAEATPNTEMTLVV
                     DLDLDLLKQLQTQGSVRNMKSRRHDLYKIHMFDK"
     gene            complement(3156..4640)
                     /locus_tag="PLF66_25400"
     CDS             complement(3156..4640)
                     /locus_tag="PLF66_25400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67450.1"
                     /translation="MRYLLLLVFVLILPIGLISNGITFIEAIAKLEGKDKSSTSEFLN
                     LLVNIASRTKFNSQAEEFHEYLSKSLEKFEFKDLYNSKYMKSSNNGYKRVNYGGLNSK
                     EGLFVNLIETEFEKIPIQNFGDFASSLSLKKNSLSSVTKGSYAKSNSYSGDLKLDQNF
                     GIFSHNKGANLLNSLLSVIDINNLEKTNYPPTKTFKNLKNTENRKLLELIANDLPSVS
                     RFLNQFFDADSILKESKTNDKRPTLFVLNGRINNRHVQNQYPYLAGYFDDISDLGWIH
                     TVLTDSLGNKIFEINLESESLLLYVKFYTLEGKIIPYKTQDGIDTLDLSKAFSIPNLT
                     SYNFKMDISFEGRIYGLKLKTNHILIQGLFTNRAEEGSLYFILNEFPKTSVSGGFSYI
                     IPSWAIDLAIPGNMEELIEHFTSVLLHANQEKGSFVKLNWKKKANWIFSIRSESEFVD
                     NFFIRFGLKVFNYKIKPNQKAGEELSQFLVQILELTKQDLNAKL"
     gene            complement(4644..>5658)
                     /locus_tag="PLF66_25405"
     CDS             complement(4644..>5658)
                     /locus_tag="PLF66_25405"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HMZ67451.1"
                     /translation="EFNILSLDEVGSIQRSLHSFSNRLSSIIRNDITIANTLASSADA
                     MHNSLNSLSSNTQTQAASSEEISASIEEISAGIDSVNGRAEDQFSKVKILDDKMQELT
                     ETITSMADEIANISRNVSTIVKDASKGEKSLDQMNNSISNISASSKQITNVMEIITNI
                     SSQINLLSLNAAIEAARAGESGKGFAVVADEIGKLADKTSRSIKDISSLVHTNQNEIS
                     QGSKIIQDTIQIIHKVISGVNSFHSMAETLELQVKMQKSINKVANDELNSLNQMTSLI
                     RSAMQEQKLAIEEVAKTIYGINEITQSTAAGVEELNATSEEIAATAESLKKEMEFFKI
                     KSD"
BASE COUNT         1781 a          886 c         1026 g         1965 t
ORIGIN      
        1 aaactctcat cactgattct ttttttcctt tctatgtttg agtaggttct ctttttcttc
       61 aggatctgta gtgctaacaa cacctacagg tttattgtaa gcgaggataa tcgttgtttt
      121 cctctgcttt gcttctattg tatatccatt gactacaacc ttatcgcctg taaatacacg
      181 gtctcctaca cttgcctttc tgccattaat aaagacagtt cctagttgaa tgtattcatc
      241 tgcttctctt ctagagcata gacctgattc actgatgtat ttattgagtc gagtggattc
      301 tttttctagc atgaaactag gatttttatt catctagatt gagcaactta ttttgtttga
      361 gcttttattt tatccaaaat aggaatgtaa aactattgac ttaggtttgt aaagtttctc
      421 ttatagaaat aggtgaaact cggttctgta gatatttgat ctctaagaat ggagttttga
      481 aatggattca tactaaattt aattgggata ttttggagtt atcaaatgag ctttttaaga
      541 gatagaatta ttttaattac aggtgcaacg ggtggtttag gaaaagaaat ggtaaaacaa
      601 tttttaccag aaggttcaaa attgattctc actgatttga atcaggcttc tttagatgaa
      661 ttaagttcaa aattggataa aaagggacag atacttttta cattcacaag tgatttatcc
      721 acacaagagg gttgtcaaaa agtttacgat acaatcaaat caaagaatta tgaaatagat
      781 attttgataa ataatgcagg tgttgcgtct attggtaatt ttatgacaac accacaagaa
      841 gaatgggaaa gacaatatgc aattaatctt ttagctccta ttaggcttac taaactcttt
      901 ctgactgata tgcaaaaaag aaattccgga cacttggtaa acatagcgtc tgtcgcttca
      961 tttgtatctg ctcctggtct tgcaacatat tcttctacaa agcatgcact gagggctttt
     1021 ggtgaatctc tatcagaaga attaaaacct actaaaatta gagttaccaa tctttatccg
     1081 ttttttacaa agactccaat gatggaatct cctcaatatg gttttaaaaa taaaaaagaa
     1141 attccgtctc tcttaatgtc aactccagag gaagtaattc gagatatgat taatggaatt
     1201 aaaagagatg agctacatat attacctgga ccagtcgcaa aggcaaccga gtttttaaat
     1261 cggattgctc ctcaaggttt gagtttgttt tttgattttt tgagataaga atatggaaac
     1321 aaatacaaaa aagaaatcta aaaaaaaatc ggatgagaga aagggctcaa aacctgtcgc
     1381 aaagaaaaag ccaacaagtc tcaaattaaa acttagaaat actcgtccag ccgattatga
     1441 gagtattaaa gagatcatgg atatggtcta ttcaaatgca ggcggagctt ggaagcaaga
     1501 agagttttta tctcagctag aacattttcc ggaaggacag ttttgtattg aggataacgg
     1561 aaagttagtc gcagccgttg tgagtatgat cgtaaaatat tcgaattttg gggacaggca
     1621 tacatatgat caaattacaa cggaaggata tttacgaaat catgatccag aaggagatac
     1681 gttatacggt gtagatattt ttgtgcatcc agcctaccaa ggacttcgtt taggcagacg
     1741 tttgtatgat gcaagaaaac aactctgcca gaatctaaac ctaaagagaa ttattatagg
     1801 gggaagaatt cccaaataca aagatcattt taccgaaatg tccccgcaag agtatgttac
     1861 aaaggtaaaa gaaaaagaga tctttgatcc agttttaagt tttcaattgg cgaatggttt
     1921 tcatgtgaga agaatcatga caaactattt cccagaagat aaagattcta aaacatatgc
     1981 aactctttta gagtggctga atatttatta tgaagagaaa gaacaactca ttggaggaaa
     2041 aaagccaatt gtgcgagtag gggttgtgca atggcaaatg cgatctgttg attcctttga
     2101 agctttctta aaacaaattg aattctttgt agatgcagtg gcaggttata aagcagattt
     2161 cgttttattt cctgaatttt ttaatgtccc gctcatggca aaatacaata aactaaatcc
     2221 tgccgatgcg atacgatctc tcgctgacta tacagaaaaa attagaacag aaatgcttca
     2281 tatggcagtg tcgtataata ttaatattat ttcaggaagt atgcctgttt atacagatga
     2341 agtgttgtat aacgtttctt atttactgag aagagatggg acttgggacg aacaatataa
     2401 aattcatgtt actccggatg aagaaaatta ctggggagtc aaagggggaa ccaaagtaaa
     2461 aatctttgat accgatgtag gcaaaattgg aattttaatt tgttttgatg tagaatttcc
     2521 tgaacttcct cgaattttag cagaacaaga tatggatatt ttatttgttc ctttttccac
     2581 ggatacaaag aatgcatact tacgcgttag gcactgcgca caggcaagag caattgagaa
     2641 tgaatgctat gttgtgattt ctggaagtgt aggaaatcta cctgatgtag agaatatgga
     2701 tattcaatat gcacagtctg cagtatttac tccatcggat tttgctttct ctcatgatgc
     2761 gatagccgct gaagctactc caaacacaga gatgacactt gttgtagatt tggatttaga
     2821 tttactcaaa caacttcaaa cacaaggtag tgtccgaaac atgaaatcgc gtcgacatga
     2881 tttatataag attcatatgt ttgataaata gtggattgtg tggagtagtt ttaccacgga
     2941 tgaatatgcc aatgcttgca gcgtagggaa gaatagggat ggatgatatg atttttacat
     3001 tgttgttttt ctctttagac aagctgcaca ttggcgcctc taagatttgt cataaagcac
     3061 cattttgatt gttttcctat tagactttct ctctttgctt gctatagtag aaactgaata
     3121 taataggaga agtttgaatg tgtaagcgtt atactctata gctttgcatt taaatcttgc
     3181 ttagtgagtt ctaggatttg aactaaaaac tgggaaagct cttctccagc tttttgattt
     3241 ggttttattt tataattaaa aactttgagt ccaaagcgaa tgaaaaaatt gtctacgaac
     3301 tcgctctcag agcgaataga aaatatccag ttcgcttttt tcttccaatt gagttttaca
     3361 aaacttcctt tttcttggtt tgcatgtaag agaacacttg taaaatgttc aattaattct
     3421 tccatatttc ctggaattgc aagatcaatt gcccaagaag gaattatata agagaatcct
     3481 cccgatacag aagtttttgg gaattcattt aaaataaaat ataaactacc ttcttcagct
     3541 ctatttgtaa ataaaccttg gattagtata tggttagttt ttaatttgag tccatagatt
     3601 cttccttcaa aagaaatatc cattttaaaa ttgtaagaag ttaaattggg aatggaaaat
     3661 gcttttgaaa gatctaacgt atcaattcca tcctgtgttt tatacggtat gatttttcct
     3721 tctagtgtat aaaactttac atatagcaag aggctttcgc tctctaaatt gatttcgaat
     3781 attttatttc ctaatgaatc tgtgagaaca gtatgaatcc atcctagatc agagatatca
     3841 tcaaagtatc cagctagata aggatattga ttttgcacat gcctattatt gattctacca
     3901 tttagaacga agagagtagg gcgcttatcg tttgttttag attcttttaa aattgagtct
     3961 gcgtcgaaga actgatttaa aaatcgactt acagatggta aatcatttgc gatcaattct
     4021 agcaatttcc gattttctgt attttttaga ttcttaaatg tttttgtagg agggtagttg
     4081 gttttttcaa gattgttaat gtcaattaca gaaagaaggg aatttaaaag gtttgctcct
     4141 ttattatgag agaaaattcc aaaattttga tctaatttta aatctcctga ataagagttt
     4201 gatttcgcat aacttccttt tgtaacagag gagagtgagt ttttcttgag tgatagagag
     4261 gaagcaaagt ctccaaagtt ttgaattggt attttttcaa attctgtttc gattagatta
     4321 acaaatagac cttctttaga gttgagtcct ccgtaattga ctcgtttgta tccattgttt
     4381 gaagatttca tatacttgga attgtataaa tctttaaact caaatttttc taaagattta
     4441 cttaaatact catgaaactc ttctgcctga gaattaaatt ttgttctact tgctatattg
     4501 actagaagat ttaaaaattc ggaagtagag cttttatctt taccttctag ttttgcgatt
     4561 gcttctatga atgtaattcc attcgaaata agtccaatcg gaagtattaa aacgaatacc
     4621 agaaggagaa gatatctcat accttaatct gatttaattt taaaaaattc catttctttc
     4681 tttagtgatt ctgctgttgc tgctatttcc tctgacgttg catttaactc ttcaacgccc
     4741 gctgctgtag attgggtgat ttcattgatt ccatagatgg tttttgcaac ttcctcaatc
     4801 gcaagttttt gctcttgcat agcagacctt atcagagatg tcatttggtt taagctattt
     4861 aattcatcgt ttgctacttt attaatcgat ttttgcattt ttacttgtaa ctctaaggtt
     4921 tccgccatgg aatgaaaaga gtttactcct gatattactt tatgaataat ttgaattgta
     4981 tcttggatga ttttacttcc ctgcgagatt tcattttgat ttgtatgaac cagagaggaa
     5041 atatctttta tacttctaga agtcttatcg gctaactttc caatttcatc tgctacaact
     5101 gcaaatcctt ttcctgattc tcctgctcta gctgcctcaa ttgctgcatt caaagagagc
     5161 aaattgattt gagatgaaat atttgtaata atctccatga cattggtgat ctgtttagaa
     5221 ctagcactta tatttgaaat gctattattc atttggtcta aagatttttc ccctttgctt
     5281 gcatctttta caattgttga aacatttctg cttatattgg ctatttcatc agccatagat
     5341 gtaattgtct ctgtgagttc ttgcatttta tcgtctagaa tcttgacctt tgaaaactga
     5401 tcttcggctc taccatttac gctatcaata ccagcagaaa tttcttcaat agatgcggag
     5461 atttcttctg aggatgcagc ttgcgtttga gtattactcg aaaggctatt caaggaattg
     5521 tgcattgcat cagcagagga ggctaacgta tttgcaattg ttatatcatt tcgtataata
     5581 ctggataaac gattagaaaa agaatgaagg cttctctgta tactaccaac ctcatcgagt
     5641 gaaagaatat taaactct
//
LOCUS       DAOQOS010000222         5563 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_8816_length_5563_cov_5.999455, whole genome shotgun sequence.
ACCESSION   DAOQOS010000222 DAOQOS010000000
VERSION     DAOQOS010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5563)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5563)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5563
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8816_length_5563_cov_5.999455"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(101..1906)
                     /locus_tag="PLF66_25410"
     CDS             complement(101..1906)
                     /locus_tag="PLF66_25410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67452.1"
                     /translation="MLFSKLPRKQKYFLGVGSLILLLLALYWMAGSKGAGPISELAKS
                     VSNNDHYLSSKVIPESDLPPEGTRSLFDHLIVQNGGIVPYPYSKLIAMLKELNPAGAE
                     PINLLIPNGRSLLKGQADNAHPRIVSTADFETANSGNNLGLNLRGQLFLGFVENGHEI
                     EVISYNEAAGRFEFQLVQDYCQDCSPRLVYARRAICSTCHQGGTPIFSQRPWNETNGQ
                     PATANAIAEARKSSEPYETVPIQQPLSFPERFDQLTDVGNFYYATQRLWLDACGEKGN
                     ECRRKMLSLAFRYLDNAGGFNTKSSEANELRKYQEEVFKVSDIPLRDPKDYNYTALPI
                     ALQGKPEAKKGILVPESDLANRDPIGERQGLKGWLKSLFTRDIKFGEGAKDNEDLSSF
                     DKLPPLPKELDPLTIRAPKQIVGPQDIDGVYGLASFFTESDVKLVSSMNGYSIERIVD
                     KIQKLPAEIFDPKPFSRILIMQALVGKKDYCCLNTNDMSPPVVSGGPPLEIKNHPELQ
                     AFADNCFSCHRGNPAARLNFMSGATEEEVLEKIKAKKEIRDALDWERYEGTDKSSKLM
                     PPRNSAQYRRLTEAGEAGKKAIQQMRDTVPGFFNF"
     gene            complement(1920..4367)
                     /locus_tag="PLF66_25415"
     CDS             complement(1920..4367)
                     /locus_tag="PLF66_25415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67453.1"
                     /translation="MNKKKWGIGAVSVLGFVIVFHFATLPPAPPEVPPVPAEIKTPVV
                     PKLTAYDEPTGNVPVSAEPFRSTNRSVSIAHLVERNLGTGVYQGTPYWKQRAEWFDKN
                     FYATLAARKVWHAGTSPDTHKRGGKVTWDYEPIPQYEPPPCDKPQPTGYDAEGCRYVM
                     GLFKDVEEKDPAKAKQMREQARRGRDVWYKGTFGNQDENYLLQSRIVGKENMYYPWLD
                     TRTRKDRFTKWGLINDPDCVEGDASTNWYDKCQDPHSTGVLGYRKYYADPIKDKEGKV
                     VYDPRSAPYSADEIKQNKRYVIGHPCVQCHVGFDPTNPPENPNEPKWSNLSATIGNQH
                     IRQPLAFFHGVPMSHLANQAVLAGRLGTIDTSLVANDWQHNPGTQNNIMDFFNKRLFD
                     HEIKDPVTGKVSKAKSRHVLKGGEDSVGEHLALIRVYVNIGMCTEECWTPNFPFPGQL
                     IGRGTTQSPFKIMQCANQCDAWNYADAKMDDLAAFLITGGPTYLMAAKDKDGKDGKSY
                     IDTSLVPKGREIFAKECASCHSSKVAPENVRNDKNALAKFYEGHVFGKEDYWELEFTE
                     AKRKDPEFVAKYLVKDEKGKLRPKQFAENGVFGQDWLGNDERTLHTIVGTNMCRALHD
                     NHSQGHIWEEFASETYRNSPSAGSVKRVFNRMIPLLGGTQFGERKIEGGSGYYRNISL
                     LSVWATAPFLHNNAVGYIDDQTALDYSVKGRIKQFEIAFDELMTSDNPSITPHRPQRI
                     TTVSEDMYVTPREDAQGFPKIPVKKGTPVANLLSQDPHHPLFMKCDDQVENKGHQFGV
                     DLPAADKKALREFLKMM"
     gene            complement(5250..>5563)
                     /locus_tag="PLF66_25420"
     CDS             complement(5250..>5563)
                     /locus_tag="PLF66_25420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004786293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HMZ67454.1"
                     /translation="QLAHPIITVTMGSEKYLEIREEIVKRMSEVAPKSIEKIEKYVEK
                     ATDIEETMASRMKALPPEEFESILRSAFQEDELLLIIIGAVLGAMVGLGQAVYMIAFP
                     G"
BASE COUNT         1645 a         1120 c          947 g         1851 t
ORIGIN      
        1 gtatcttgca aaaataggtt ttgcttctat agtaattatc tgaattaaaa atacagatag
       61 aatcgcgaaa tggtttaaac gcattttaat ctcctactta ttaaaagtta aaaaatccag
      121 gaactgtatc tcgcatttgc tgaattgctt ttttaccagc ttctccggct tctgttaatc
      181 tacgatattg tgctgaattt cgaggtggca taagtttact agacttatca gtcccctcat
      241 atctttccca atcaagtgca tctcgaattt ctttcttggc ttttattttc tcaagaactt
      301 cctcttctgt tgctcccgac ataaagttta gacgagcagc agggttgcct cgatgacagg
      361 aaaaacaatt atctgcgaat gcttgtaact ctggatgatt ttttatttca aggggtggtc
      421 ctcctgaaac aacaggtggt gacatatcgt ttgtatttaa acaacaataa tctttttttc
      481 ctacaagtgc ttgcataatt aaaattctag agaatggctt tggatcaaaa atttctgcag
      541 gaagcttttg aatcttatct acaattcttt caatgctata tccattcata gaacttacta
      601 atttcacgtc agactctgtg aaaaaacttg ctaacccata cacgccatca atatcttgcg
      661 gtccaacaat ttgttttggt gcgcgaatag tcagtgggtc aagctccttt ggaagaggtg
      721 gtaatttatc aaaagaactg agatcttcat tgtcttttgc tccttctcca aattttatat
      781 cgcgtgtaaa tagagattta agccaacctt tgagtccttg tctttcgcct atcggatctc
      841 ggttagccaa atctgattct ggaactagga ttcctttttt tgcttccggc ttaccctgta
      901 aagcaatggg aagtgctgta taattataat cttttggatc tcgcaaaggg atatccgaaa
      961 ctttaaaaac ttcctcttgg tatttacgta attcatttgc ttcagagctt tttgtattaa
     1021 agccaccagc attatctaaa tatctaaatg ctaaagatag catttttcta cgacactcat
     1081 ttcccttttc accacaagca tctagccata aacgttgtgt ggcataataa aaattaccaa
     1141 catctgtcaa ttgatcaaat ctttctggaa aagagagtgg ttgttggatt ggaacagtct
     1201 cgtaaggctc actactttta cgtgcttccg caattgcgtt tgcagtcgcc ggttgtccat
     1261 ttgtctcgtt ccaaggtctt tgagaaaata taggagtgcc tccttgatga caagtagaac
     1321 agatagctct tcttgcataa acaagtcttg gcgaacagtc ctggcaataa tcttgcacta
     1381 attgaaattc aaatctaccc gcagcctcat tatagctgat cacttcaatt tcatgaccat
     1441 tttctacaaa tcccaggaaa agttggcctc ttagattcaa acctagatta ttccctgaat
     1501 ttgcggtttc aaagtcggca gtggatacaa tacgtgggtg agcgttatcc gcctgaccct
     1561 ttagaagaga tcgtccgttt gggattagga gattaattgg ttcagcacca gcagggttta
     1621 attccttcaa catagcaatt aacttgctat atggataagg aacaatccca ccgttttgaa
     1681 ctattaaatg atcgaacaac gatcgtgtcc cttctggggg taaatcagat tcaggaatta
     1741 ccttgctaga aagatagtga tcgttattcg aaacagactt tgctaattca gaaattgggc
     1801 ctgctccttt gctgcctgcc atccaataga gagcaagcaa caatagaatc agagaaccaa
     1861 ctccgagaaa atatttttgt tttctaggaa gttttgaaaa aagcaaagag taacctacct
     1921 tacatcatct tgaggaattc acgtagagct tttttatcag ctgccggtaa atcaacccca
     1981 aattgatgtc ctttattttc aacctgatca tcacatttca taaacaaagg atgatgagga
     2041 tcttgagaaa gtagatttgc tacaggtgtt ccttttttaa ctggaatttt tgggaatcct
     2101 tgagcatcct ctctcggtgt tacatacata tcttccgaaa cagttgtaat tctttgtggt
     2161 ctatgcggag taatacttgg attatctgat gtcattagct catcaaatgc gatttcaaat
     2221 tgtttgattc taccctttac agaatagtca agagcagttt gatcatctat gtaacctacc
     2281 gcattattat gaaggaatgg agctgtcgcc caaacagata agagagaaat gtttctgtaa
     2341 tacccagaac caccttcaat ttttctttct ccaaattgag ttccacctag taaaggaatc
     2401 atacggttga atacacgctt aacgcttcca gccgaaggac tattcctata tgtttcagaa
     2461 gcaaactctt cccaaatgtg accttgactg tggttatcat gcaaagctct acacatatta
     2521 gttccaacga ttgtatgaag agttctttca tcatttccta accagtcttg accgaatacc
     2581 ccattttcag caaattgttt tggacgaagt ttgccttttt catctttaac taaatacttt
     2641 gcaacaaact ctggatcttt tcgttttgct tcagtgaatt ccaattccca ataatcttct
     2701 tttccaaata cgtgaccttc ataaaattta gccagagcat ttttatcatt tcgaacattc
     2761 tcaggagcaa cttttgaact atggcaacta gcgcattctt tggcaaagat ttctcttcct
     2821 tttggaacta aggaagtatc aatataactt tttccatctt ttccatcttt atctttagca
     2881 gccattaagt atgttggacc gcctgtaatt aagaacgctg ccaagtcatc catttttgca
     2941 tctgcataat tccatgcatc gcattgattt gcacactgca taattttaaa tggggattgt
     3001 gttgttcctc ttcctattaa ttgacctggg aatggaaagt ttggagtcca acattcctct
     3061 gtacacattc caatatttac ataaacacga atgagtgcta gatgttctcc aacggaatcc
     3121 tctccgccct ttaatacgtg tcttgattta gctttgctta ctttaccagt cactggatct
     3181 ttaatttcat gatcaaacaa tctcttgttg aaaaagtcca tgatattgtt ttgggttcct
     3241 ggattgtgct gccagtcatt agcaactaac gatgtatcga tagttccaag tcttccagca
     3301 agaactgctt gattcgctag atgactcatt ggaacaccat ggaaaaacgc caatggttgt
     3361 ctaatatgtt ggtttccaat ggtagcactt agattactcc atttaggttc atttggattt
     3421 tcgggaggat tggttggatc aaatccaacg tgacactgaa cacaaggatg tccaatcaca
     3481 taacgtttat tttgcttgat ttcatccgca ctgtaagggg cagatctagg atcataaaca
     3541 accttcccct ctttgtcttt gattggatca gcatagtatt ttctataccc gagaactcca
     3601 gttgaatgtg gatcttggca cttgtcatac cagtttgtag aagcatctcc ctctacgcaa
     3661 tctggatcat tgatcaaacc ccatttggta aatcgatctt tacgagtgcg agtgtctagc
     3721 caaggataat acatattttc ctttccaaca attctagatt ggagaagata attttcatct
     3781 tggtttccaa aagttccttt gtaccaaaca tcacgacctc gacgagcttg ctctctcatt
     3841 tgctttgctt ttgctggatc tttttcttct acatctttaa agagacccat aacataacga
     3901 caaccttctg catcgtagcc tgttggttgt ggcttatcac aaggaggagg ttcatattga
     3961 ggaatcggtt cataatccca agtaactttt ccaccgcgtt tatgagtatc tggtgaagtg
     4021 cctgcatgcc agacttttct tgcggctaac gtagcataaa aatttttatc aaaccattct
     4081 gctctctgct tccagtaagg tgttccttga taaactcctg ttcctaagtt tctctcaact
     4141 aaatgtgcaa tcgaaacact tcgatttgta gatcgaaatg gttctgcaga aacgggaacg
     4201 ttaccagtcg gttcatcata ggctgtcagc tttggaacta ctggagtttt tatctcagcc
     4261 ggaacaggtg gtacctcagg aggagccggc gggagtgtag caaaatgaaa aacgatgacg
     4321 aatcctaaaa cggaaacagc acctataccc cattttttct tgttcatatt gaatcctcaa
     4381 attgtttttg tttttttatt cttgatttgc ctactaagca aatgttaaga cttaactcca
     4441 gaagatagga aaggaaagct caacggaaag attacgcttg gtatcataaa aaatagtttt
     4501 acaaaaaaaa gtctagtcaa aaagacaatt tttactaaaa aatatatttt atttactctc
     4561 cattcaaaca aatgcataaa aaggaaagac aagcaactac tttagcggaa gcaattccct
     4621 tgtatttatt aattcattag aaaatcgtaa tataattaaa tctactcttc tacttatcta
     4681 attctctttt ttacgcaatg tctgttattg ataaaaattg aatagcacca aatatttcta
     4741 gagtggatta ctttgattaa ccgccttaac atccgtttaa aatggattca ttcagaagga
     4801 tttgtatttc tttggtgttt tttagaataa tttaaaatat tctcttttca aaagatccag
     4861 aatgaaacca tcacaatcga aaccatattc atttgattca aaaacaaaca tatgagtcaa
     4921 ggttacgtta aacgttcctt aaaaagaaag aatcctcttt ataaataact gctgttaaat
     4981 gatggctaga acaatggaga ttttaaacct tttagggaat aagaatgagt tattcaaatc
     5041 aatgacttgt ggttaactca ataaaaaaag gaaaaagcga tgagtgaaaa gccctctctt
     5101 tcctcttcta aaagctcata gccatttttc aatgacaatg ggagtaaagc ttattgtaat
     5161 ttttggtgct ctcatttctt tagcaaagca atgagagcac caaaggcaaa taaataattt
     5221 taaaaaggat atagtttaaa ttagattttt tatccaggaa atgcaatcat gtaaacggct
     5281 tgacccaatc ctaccattgc acccaaaaca gcaccaatta taataagcaa taactcatct
     5341 tcctgaaatg cagatctcag aattgattca aactcttctg gtggcaatgc tttcattctg
     5401 cttgccattg tctcctcaat atctgttgcc ttttcaacat acttttcaat cttctcaata
     5461 gattttggag ccacttcgct cattcttttc acaatttctt ctctaatttc taaatatttt
     5521 tcagatccca tcgtaactgt aataattgga tgagctaact ggg
//
LOCUS       DAOQOS010000223         5545 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_8862_length_5545_cov_6.482878, whole genome shotgun sequence.
ACCESSION   DAOQOS010000223 DAOQOS010000000
VERSION     DAOQOS010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5545)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5545)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5545
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8862_length_5545_cov_6.482878"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            275..1750
                     /locus_tag="PLF66_25425"
     CDS             275..1750
                     /locus_tag="PLF66_25425"
                     /inference="COORDINATES: protein motif:HMM:NF017106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P83/100 family protein"
                     /protein_id="HMZ67455.1"
                     /translation="MGQFYKQIIKSISFLVILGISTGSLVAQKSGKKATKTSKTAKTT
                     TTTTTSTTTKTTKTTTTVEKKPLETKISAEEQKKQDINTGKKLSSGIKTDAMKTHNVL
                     GLSIKRYISNEEGKLGADVLEVTGESKVELSSVVTVLNSYIADNFEYTNEEAETLTNS
                     VILYNTKNRNNQDYLIKNYSTNVVLGVKKEKIGIPTVATAESISGQSQILIPTEKNIL
                     KKLSLDVASLELLDQTKTDLTLQKDGDEQRKKFDTFLTKKSAAENEELNNRMMTANPA
                     EIKGITDRLALNKRREEMRKANITSEKEYVAFLDSKNKKETTTSTKTTTTSTTITNTV
                     VNSALPAGPEVPKDKFTSFIQGSTNSGINARELLIIPGSGILAIGYDASLEKKELRLF
                     MTSTGDYDVKRSSEGIKLAPESPMVYSDGRIYVIEILKNEAYIAQFNTELEFELRSEA
                     TIHPKSLIQVVEDEVIVTAIEKKGTKDDVKVFKVKDLSLVR"
     gene            1876..2082
                     /locus_tag="PLF66_25430"
     CDS             1876..2082
                     /locus_tag="PLF66_25430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67456.1"
                     /translation="MISKNFNNLVVTTFFEGFCILRSHEGKIGRISVENKSIEKWKVI
                     DINSNQVLESFSSIQDLLKAGWSV"
     gene            2336..2665
                     /locus_tag="PLF66_25435"
     CDS             2336..2665
                     /locus_tag="PLF66_25435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67457.1"
                     /translation="MFLADMDIINYITNDLIHYFDIGYILLVVVTNFALLNKTSLKTK
                     LTNPKKQFFKLGVAIVYAIFFLSFNYFSHGTKRALPYTETILVSLFVSLIIYETVARI
                     LKDKLKK"
     gene            3099..3512
                     /locus_tag="PLF66_25440"
     CDS             3099..3512
                     /locus_tag="PLF66_25440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004499720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67458.1"
                     /translation="MLDIKLLEGEQKVFELEADFWNRGSNPIQKFFGNVLRVISKILG
                     HKIHGKLIVTNMRALEVKETIDWYCFSTKKEVKLLTKTSIKEIGYEMEKVCLVFCPTY
                     SLYYEGTTQSTSIAIENGSDDLLIDLIGKFYNVIK"
     gene            3628..4068
                     /locus_tag="PLF66_25445"
     CDS             3628..4068
                     /locus_tag="PLF66_25445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67459.1"
                     /translation="MITFFDKLRLNLFPILLLSISFLFVIGIFIMESIYLRIIFLMLI
                     FQNINIAFKLLKTYKSKIRSILMLTHRNKNSFKGETFKKYMKAPCGRQVVKISLQKIS
                     KQSKYKELKRQYPMKIISLRNEKAKVIYYSEGKILNADKNNRID"
     gene            4040..4465
                     /locus_tag="PLF66_25450"
     CDS             4040..4465
                     /locus_tag="PLF66_25450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67460.1"
                     /translation="MRIKIIESIKLPMNGFRIFITIILFLSLLRNPIDSKENERILIK
                     ASVIEFEKREEREYSMDGGGCDLLLHVKAMVLILETNPRKILRKKMTIKKSYVYDSKE
                     IRILYKMEKGSIIQFSSFDPVNPDGEIEVHEFLSIENPK"
     gene            4845..5045
                     /locus_tag="PLF66_25455"
     CDS             4845..5045
                     /locus_tag="PLF66_25455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010416321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HMZ67461.1"
                     /translation="MEKCHCARVSFDQIIQLASRDNRPYKQVAKDLNAGETCTACRED
                     MKAYCESVCGKSNTQLQLQVAI"
     gene            complement(5011..>5545)
                     /locus_tag="PLF66_25460"
     CDS             complement(5011..>5545)
                     /locus_tag="PLF66_25460"
                     /inference="COORDINATES: protein motif:HMM:NF012799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HMZ67462.1"
                     /translation="LNGIDREFELLNEHSDDVEMQRLLDLMLSKNLTCLISDAGTPGI
                     EDPGSGLVNLAVRNGMEVKIVPGPSAITCALALCGFRISPFTFIGFLNREDEKRRLEI
                     IKYLGYGHTLVLYETPYRYKKVIREIAKYAKKNYRIFLGLNLTAEDEIQYRGTLKEIL
                     PHLEGFPKSPPVIVIEC"
BASE COUNT         2123 a          839 c          871 g         1712 t
ORIGIN      
        1 taatttttcg ataccattgc aggtgttttc aacaggtttc aaacttcgta tcccgatttg
       61 attccaattt ttttagcaaa aaatcgaaac ttaggattta acgatgattt cttcgattag
      121 aagcgcaatc aaaggtaggc tgattcgtat gataatatgc ttgaataaat tatttctgct
      181 agaaaaagtg aataataagg cttacaactg aatatatttc aatctatcaa tcggggtgag
      241 tcattagtta aaaataattg gagaatacaa ttcgatggga caattttata agcaaatcat
      301 aaaaagtatt tcttttttag tgattctagg aatttcaaca ggaagcctag tcgctcaaaa
      361 gtctggaaaa aaagcaacta agacttcaaa aacagcgaaa accactacaa caactacaac
      421 ctctaccacc accaaaacta caaaaacaac gactactgta gagaagaagc ctctagaaac
      481 aaaaatctct gcggaggagc aaaaaaagca agatatcaat actggtaaga aactatcttc
      541 cggaattaaa acagatgcaa tgaaaacaca taacgtctta gggctaagca taaaacgtta
      601 tatttccaat gaagaaggaa agctaggtgc agatgtatta gaggttacag gagaatctaa
      661 agtagaatta tcctctgtgg taacagtttt aaactcttat attgctgata attttgaata
      721 cacaaacgaa gaagcagaaa ctcttacaaa tagcgtcatt ctttataata caaaaaatag
      781 aaacaaccaa gactatttaa taaaaaatta tagcacgaac gttgtattag gagttaagaa
      841 agaaaaaatt ggtataccta ctgtggcgac agccgaaagc ataagtggtc aatctcagat
      901 tctaatccca acagaaaaaa acatattaaa gaaattgtct ttagatgttg cttctcttga
      961 attattagat caaacaaaaa cagatctaac tctccaaaaa gatggagatg agcaaagaaa
     1021 gaaatttgat acattcctaa ctaaaaagag cgctgcagaa aatgaagaac taaacaacag
     1081 aatgatgact gccaatccag cagaaatcaa aggaattaca gatagattag ctctaaacaa
     1141 aagaagagaa gaaatgagaa aagcaaatat tacttctgaa aaagaatacg ttgcttttct
     1201 agattctaaa aataaaaaag aaacaacaac tagcacaaag acaactacaa caagcacaac
     1261 cattacaaat acagttgtaa actcagctct acctgcagga ccagaagttc caaaggataa
     1321 gtttacatcc tttattcagg gttctaccaa ttctggaatc aatgcgagag aattgctaat
     1381 cattcctggt tctggaattc ttgcgatagg atatgacgca tctctcgaga aaaaagaatt
     1441 aagacttttt atgacttcaa ctggagacta tgacgttaag cgatcttcag aaggaattaa
     1501 acttgctcca gaatctccta tggtgtattc agatggaaga atttacgtca ttgagattct
     1561 taaaaatgaa gcatacattg ctcaatttaa cacagaatta gaatttgaat tgagaagtga
     1621 agcaaccatt catcctaagt cgctgattca agtagttgaa gatgaagtaa tagtcactgc
     1681 aattgaaaaa aagggaacca aagacgacgt aaaagtcttt aaagtaaaag atttaagctt
     1741 agtaagataa tttaacttaa tattgcccta tcgaaaaaat ctctccctaa aggagagatt
     1801 ttttttgctc taaccctatt ttgtcctttg atgagagtaa aataatctaa atcacagggc
     1861 aataggatag attcaatgat ctcaaaaaac tttaacaatt tagtagtcac aacatttttc
     1921 gagggatttt gtatcttgcg atctcatgaa ggcaaaatag gtagaatctc agtagaaaat
     1981 aaatccatag aaaaatggaa agttatagat atcaattcta atcaagtatt agaaagcttt
     2041 tcatctattc aggatttgct gaaagctggt tggtcagttt gattattccc aaatataaaa
     2101 atagagatat aaaacaattc cacacaaccc tctcaattcc ttatctaaac ctattttctc
     2161 tctagggaaa cgttacgtat atacataatg attaaaatca taaaaaatga ttcatatata
     2221 ctgattgata aagtaaaagc cttcttataa atacgatagt atattttgaa tagactggga
     2281 tcaatatgac ttcagtttat cgattgataa ataatttatt aaataagagg attttatgtt
     2341 tcttgcagat atggatatta taaactatat tactaacgat ttaattcact actttgatat
     2401 tggttacata cttctagtag tagtcacgaa ttttgctcta ttaaacaaaa catcattaaa
     2461 aacaaaactc actaatccta agaaacaatt ttttaagcta ggagttgcaa tcgtatatgc
     2521 gattttcttt ttgtctttca attatttttc tcatggaacc aaaagagcct tgccatacac
     2581 agaaacaata ttagtttctc tctttgtttc attgatcatt tatgaaacag tcgcaagaat
     2641 actaaaagat aaactaaaaa aataattttc ttctattcaa ggagatttgc tcgaatcaac
     2701 taatcaagac gaagaaatat tttttctctt ttatctaaag ctcatatgac tcctttctta
     2761 cataaaattt tgcgaagaaa ggatagtatc ctaattctct agcagagaac ggtatccttt
     2821 gccaattatt ttccaaattt catgttttac taatagggaa cagtaaaagt ctaaatgaat
     2881 ccactcatcc aatattgaat tatttacttt agtcactctg cacagtttaa aaaactaaat
     2941 ctttgcaatt tttgataaaa gaatagtttt ctattatact ccacttaatg gattaatttt
     3001 gtatataata gctaatacta ctatatatcc tagtttttct attgatttat tttaagacta
     3061 ttattcctta gtaatttatc ttaaattaag gagtaaaaat gttagatatt aaattattag
     3121 aaggggaaca aaaagtattc gaacttgaag cagatttttg gaatagaggc agtaatccta
     3181 ttcaaaaatt ttttggaaat gtattaagag ttatctctaa aatcttagga cacaaaatac
     3241 atggtaaatt aattgttact aatatgagag cattagaagt gaaagaaact attgactggt
     3301 attgcttttc aacaaaaaaa gaagtgaaac tactcacaaa aacaagtata aaagaaatag
     3361 gatacgaaat ggaaaaagtt tgtttagtat tttgtccaac ctattcttta tattatgagg
     3421 gaacaacaca atctacatct atagcaattg aaaatggatc cgatgattta ttaattgatc
     3481 taattggtaa gttttacaat gtaataaagt aattttatgc attgtaactt aaaaaaataa
     3541 aaagatcaga gaatcatcta aaatcctaga ctttttaaat tcgatagtca ccctattaaa
     3601 cattagggtg acatttctgt caaaaaaatg attacgtttt tcgataagct tagattaaac
     3661 ttatttccaa ttttactttt atctatatcc tttctatttg ttattggaat atttataatg
     3721 gaaagtattt atttaagaat tatttttcta atgttaattt ttcaaaatat aaatatagca
     3781 ttcaagttgt taaaaacata taaatctaaa attagatcta ttcttatgtt aactcatcga
     3841 aataaaaata gctttaaagg cgaaacattt aaaaaatata tgaaggcgcc atgcggtagg
     3901 caagtagtaa aaatatcgct ccaaaaaatt tctaaacaaa gcaagtataa agaattaaaa
     3961 agacagtatc caatgaaaat aatttcactc aggaatgaaa aagcaaaagt gatatattac
     4021 tctgaaggaa aaatattgaa tgcggataaa aataatcgaa tcgattaaat taccgatgaa
     4081 tggattcaga atttttataa caatcatact ttttctatca ctgctaagaa atccgattga
     4141 ttcgaaagaa aatgaacgca ttttaataaa agcaagtgta atagaattcg agaaaaggga
     4201 agagagggaa tatagtatgg atggaggtgg atgcgatctt ttgctccatg taaaggcaat
     4261 ggttttaatc ctagaaacca atccaaggaa aattctaaga aagaaaatga caattaaaaa
     4321 gtcttatgta tatgattcga aagaaatcag aattctatat aaaatggaaa aaggaagtat
     4381 cattcaattt tcaagttttg atccagtcaa tcctgatgga gaaatagaag tgcatgaatt
     4441 cttaagtatt gaaaatccta aataaattcc caagtaattt gtatttcatg tgagttgttc
     4501 ctttatttaa aatccttttt agatgattct tttaagcaat gcaaaaaaag aaagagccac
     4561 gcaatgcatt tcatttttag agaaattttg aatcgaatag tttggtatac gaatcgaaat
     4621 gagagattgg aaatttttta gaattgtaga tagatataag atatcaaatt attttataga
     4681 aacaaagctt caaaaggaac tgtcacaaat tcttgtataa ggatatatac cgattccttc
     4741 ctctccttaa tactggaatt tatgataaaa atcagtatca ataacatgtg agtagacaaa
     4801 aaaagacttg acaatgagaa caggtctcaa tatcgtattc acatatggaa aaatgtcatt
     4861 gcgctcgtgt atcattcgat caaatcattc aactcgcttc tcgcgataat agaccttata
     4921 aacaagtagc aaaggattta aatgcagggg aaacttgcac tgcctgtaga gaagatatga
     4981 aggcttattg tgagtctgtt tgtggtaaat ctaacactca attacaatta caggtggcga
     5041 tttaggaaaa ccctctagat ggggcaatat ctcctttaaa gtccctctat actggatttc
     5101 atcctcagct gttagattga gtcccaagaa gattctataa ttctttttgg cgtatttagc
     5161 aatttctcga atcacctttt tgtatcgata cggtgtctcg tagagcacaa gggtatgccc
     5221 ataacctaga tattttatta tttccaatct tctcttttcg tcttctcgat ttaaaaaacc
     5281 aataaatgta aaaggagaaa tccgaaatcc acaaagagca agtgcacaag taatcgcact
     5341 gggtcccggg acgatcttta cctccatacc atttcgaaca gctaagttga caagtccaga
     5401 gcctggatct tcaatgcctg gagttcctgc atccgaaatg aggcaggtga gatttttgga
     5461 aagcatcaga tcgagtaagc gctgcatttc cacatcgtct gaatgttcgt ttagaagttc
     5521 aaattctcta tcgattccgt ttaac
//
LOCUS       DAOQOS010000224         5459 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_9057_length_5459_cov_5.132494, whole genome shotgun sequence.
ACCESSION   DAOQOS010000224 DAOQOS010000000
VERSION     DAOQOS010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5459)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5459)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5459
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9057_length_5459_cov_5.132494"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(40..861)
                     /locus_tag="PLF66_25465"
     CDS             complement(40..861)
                     /locus_tag="PLF66_25465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67463.1"
                     /translation="MKLLSIILFFFSFMSGVIYGQEGYYSLSYKMNSVVAYVELNGIK
                     QTESGKEGFLSGVSTGLNLWIMPGENTLKIKLTKPQKGKNQDNPQIKVAIRLGTAGQS
                     TEEGKEIVSFEAPESEDSKIEFPLEKEVKFTPELIPPSSLWEKVEPTNLDSTSKKEIT
                     ELVQALHKAANTANVKDFFKLIEFKMQDMSKAMYSNPEENKNDKALKEFLKTIKGKLK
                     KLPPKLSFHPVANGKLIFVTDSNGNDPIQSKPSQDGEFNISVYVGKIDGKWTLVR"
     gene            1422..2525
                     /locus_tag="PLF66_25470"
     CDS             1422..2525
                     /locus_tag="PLF66_25470"
                     /inference="COORDINATES: protein motif:HMM:NF018190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HMZ67464.1"
                     /translation="MNFSETIWILILALVLSCSMDNSPKLKGFFPNVNATASNQQVDS
                     CISATPSNPTSVTITTTTSPDSIRMQWTNPTDCPIGGVIVRRKTGSSPSDYKDGTSIS
                     VNSNNTSFTDNSVSPSVLYYYKIFIYNTAGTLYSSGLSAVAIAGSTSVVPTKVGDNVF
                     TLDGLDSESAWASSPKISFSFPVLSSYLDYTGGGDLNVTGYIRFAYDSSNFYIFYHTD
                     DKYLRTDDTGAPWTDDSVELFFDMGYDRSTSPDTNDFKFIFTPANPSNNFFQQGNGAG
                     WSTTNPTVGQAMYTSGCTFNTDGDIDSGWNMEMKIPFSILGVSSIAQGQVIGFTFYIN
                     DDDLSAFSWAQHMFAWTTGTVHSQPNTWGILTF"
     gene            complement(2657..3184)
                     /locus_tag="PLF66_25475"
     CDS             complement(2657..3184)
                     /locus_tag="PLF66_25475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67465.1"
                     /translation="MIKLFPILFLFLFFMTENCKVYKSNSEKRNKCMQTFLAAYPQEG
                     PLYFRSGGYEFRFSCTTEGESSPTCVEYYSRTALDENFLCNVGYRRSRNRCSQQNLVG
                     SCLETQAFGSSTDGIVLVSIFAKPQNTKEEAQLYCASSNRKGNFFSVYRTPNDRSNLE
                     KAAWDAWITCTSTIK"
     gene            complement(3192..4556)
                     /locus_tag="PLF66_25480"
     CDS             complement(3192..4556)
                     /locus_tag="PLF66_25480"
                     /inference="COORDINATES: protein motif:HMM:NF019042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HMZ67466.1"
                     /translation="MYKIYLIIFFLLSILSLSAQEVESKKETVPEKKEEVIPSENSSK
                     KEEKPTEALKKEEKPAEPPQKEEKPVAPPKTPNASVDTRLGRGLYVKTEDSQFTMQMR
                     ARIQARYTYQAVSEDSKSDQEGAEIRRLRLVTRGNIYGKEWTYYIQLGVANQDMETRP
                     IPLRDAVINYNKWSDFRISMGQMKVPFNRQRLTSSSALQMVDRSIVNSELNLDRNIGI
                     QVFSENFLGLNRKFGYNLGVFHGNGRNTNLKPTGLLWVGKFTYSPFGGMTRKVFNEEE
                     NDSLSESDLSRSSDPRLAISVSVAKNNNTDRSQSTIGTIYQFAKFDYNHQCVDVFFKW
                     GGFSMMGELIRRVANAPYQEKEDTTTGRLSREYSRSAEGFFIQAGYLFPNYYEVSMRY
                     GEYRPIGKTDPNLVTSIEQGVGLSKYFEDHNLKVQADFFQIKGTPAIQGETYQFRIQT
                     QVFY"
BASE COUNT         1683 a          918 c          979 g         1879 t
ORIGIN      
        1 atgtggaaaa tgaggagtaa ccgctaataa aaattgattc taccgaacaa gagtccattt
       61 cccatctatt ttacctacat agactgaaat attgaactct ccatcttggc tcggtttgga
      121 ttgaattgga tcattgccat ttgaatcagt aacaaagatc aatttgccat tagccacagg
      181 atgaaaagaa agtttgggag gaagtttttt tagttttccc tttattgttt ttaaaaattc
      241 tttcaaagct ttatcatttt tattttcttc tggattagaa tacattgctt ttgacatgtc
      301 ctgcattttg aattcgatta atttgaaaaa atccttcaca ttcgcagtgt ttgccgcttt
      361 atgcaaagct tgaactaatt cagtaatttc ttttttagag gtagaatcaa ggtttgtagg
      421 ttctactttt tcccaaagag aggagggggg aatcaattca ggagtaaatt taacttcttt
      481 ttcgagaggg aattcaatct tagaatcttc agattcgggt gcttcaaaag atacaatttc
      541 ctttccctct tctgtggact gtcctgctgt gccaagtcgt attgcaactt tgatctgagg
      601 attgtcttga ttttttcctt tctgtggttt tgtgagtttt attttgagag tgttttctcc
      661 aggcatgatc caaagattga gtccagtgga aactcctgaa agaaagcctt ctttgcctga
      721 ctcggtttgc tttattccat ttaactcaac ataagctaca actgaattca tcttataaga
      781 caaagaataa taaccttctt gtccatagat tactccactc ataaacgaga aaaaaaatag
      841 gatgatagat agtagtttca tttctttgaa aaactcccac tataaataaa agtcaatgtt
      901 tttaatttga gttttttgga tctaagtgat atagatttac ttctaagcat acaaaattga
      961 atcaatgtaa aatcgatcat tcactgacgt tacacagatt gaagaatttt agtgtaaatg
     1021 cggattctaa taatctgaaa gaatacaaat gaaatgccta cgatagttcc gctgtttaag
     1081 ctcttagaaa gtgatggttg gaaaacccat ctatccttca tttggctcaa gtctatttat
     1141 aaaggacaat aggaggaatc ctactttgaa attttgactg atcaaaactc aatcattgct
     1201 tttttcaagc gatgagaatg aaagatgagt gatttagaag gatgaattgc tgaaaaaaag
     1261 aatcgatcgt tacatttttt tttatttcat tttcttttct caatgatttg aattgtaatt
     1321 tttagaaatt tctgttttat ttcaagtata gaaatcctgg gattgataaa gacaagaaaa
     1381 gatagataat aaagatggaa ccaagaaggg tgttaagtga aatgaatttt tctgaaacaa
     1441 tatggatact gatactagcg ttagttttga gttgttccat ggataatagt ccaaaactga
     1501 aaggattttt tccaaatgta aatgcaacag cctcaaatca acaagtcgat tcttgtatca
     1561 gtgcaactcc ttctaatcct acttcagtca ctattacaac tacaacctct cctgattcaa
     1621 ttcggatgca atggacgaat ccaacagatt gtccgatagg tggtgtgatt gttcgaagaa
     1681 aaactgggag tagtccctct gattataaag atggaacttc catttctgta aattcaaata
     1741 atactagctt cacagataat tctgtttccc catctgtttt gtattattat aaaatattta
     1801 tctataatac agcaggaact ctttattcta gcggtttatc tgcagttgct atagcgggct
     1861 cgacaagcgt tgtgcctact aaagtaggag ataacgtatt tacactggat ggattggatt
     1921 cagaatcagc gtgggcaagt agcccaaaaa ttagtttttc ttttcctgtt ttgtcctctt
     1981 atctagatta cactggaggg ggagatttaa atgtaacggg ttacattcga tttgcctatg
     2041 actctagtaa tttttatatt ttttatcata cagatgataa gtatttaaga acagatgata
     2101 ctggggcacc ttggacagat gatagtgttg aattattttt tgatatgggt tatgatcgtt
     2161 ccacttctcc ggatacaaat gattttaaat ttatatttac tcctgcaaat ccatcaaata
     2221 atttttttca gcaaggaaat ggcgctggtt ggtctacaac aaatcctact gtaggtcaag
     2281 caatgtatac gagcggatgc acctttaata cagatgggga tatagactct ggatggaata
     2341 tggaaatgaa aataccgttc tctattttgg gtgtatcgtc tattgcacaa ggacaagtca
     2401 ttggttttac attttatata aatgatgatg atctatctgc attttcttgg gctcaacata
     2461 tgtttgcatg gactactgga accgttcata gtcagccaaa tacatgggga atactgacat
     2521 tttaaattct agcaataggc tttcctttct atcttctaat ttttaggact caataaaatt
     2581 tctacctatt ctatgaaaaa agaataggct gaaattattt ctcaattcat tgactcacta
     2641 aaaaatatag tatgaactat ttgattgtgc ttgtgcatgt aatccaagca tcccaagctg
     2701 ccttttctag attgcttcga tcattgggag tgcgatagac ggaaaagaaa tttccttttc
     2761 gattcgaaga tgcacaataa agttgagctt cttcctttgt attttgcggt ttcgcaaaaa
     2821 ttgaaacaag cacaatccca tccgttgaac taccgaatgc ttgtgtctct aaacaagaac
     2881 caactaggtt ttgttgagaa caacgattgc gagatcttct ataacctaca ttgcacagaa
     2941 agttttcatc taaggctgtc ctagagtaat actccacaca agtcggagaa gactctcctt
     3001 cagtcgtaca agagaatcga aattcgtaac cacctgatct aaaatacaga ggtccttctt
     3061 gaggatacgc tgctagaaat gtttgcatac atttatttct tttttcagag tttgatttat
     3121 aaactttaca gttttctgtc atgaaaaata gaaataggaa taaaatagga aacaatttaa
     3181 tcatataagc ctcagtagaa cacctgtgtt tgaattcgga attgatacgt ttctccctga
     3241 atagcagggg ttcctttgat ttggaaaaag tcggcttgga cttttaaatt gtggtcttcg
     3301 aagtatttag aaagacctac accttgttca atggatgtaa ctaaattggg atccgttttc
     3361 ccgatcggtc gatattctcc atacctcata ctaacttcat agtaattggg gaatagataa
     3421 cctgcttgaa taaaaaaacc ttccgcagag cgagaatatt ctctacttaa tcttccagtg
     3481 gtggtatctt ctttttcttg gtaaggtgca tttgcaacac gacgaattaa ttcacccatc
     3541 atagaaaaac caccccactt aaaaaaaaca tctacacact ggtgattgta atcgaatttt
     3601 gcaaattgat aaatagttcc aattgtggac tgagatctat cagtattgtt attttttgca
     3661 acagatacac tgattgcaag tctaggatct gaactccgac tgagatcact ttcagagaga
     3721 gagtcatttt cttcttcatt gaatactttt cttgtcatgc ctccaaatgg agagtaggta
     3781 aatttaccaa cccaaagaag accagttggt tttaaatttg tatttcttcc atttccatga
     3841 aacacaccca agttgtatcc aaactttcta tttaaaccta aaaaattctc agaaaaaact
     3901 tggatcccaa tatttcgatc cagattcagt tcactgttta caatcgatct atctaccatt
     3961 tgaagagcgg atgaggacgt tagtcgctgt ctattaaaag gaactttcat ttgccccata
     4021 gagattcgga aatcactcca tttattgtaa ttaatcactg catctcttaa aggtattggt
     4081 cgtgtttcca tgtcttggtt tgcgacgcct aactggatgt aatacgtcca ttcttttcca
     4141 taaatatttc ctctagtcac gagtcgcaat cgacgtatct ccgcaccttc ttgatcggat
     4201 tttgaatctt cgctcacagc ttgatatgta taacgggctt gaattcttgc tctcatttgc
     4261 atagtaaact gagaatcttc tgttttaacg taaagccccc gcccgagacg tgtatctact
     4321 gacgcgttcg gagttttggg tggggcaact ggtttctctt ctttttgggg aggctcagca
     4381 ggcttctctt cttttttgag tgcttcagta ggtttctctt ctttttttga agaattttct
     4441 gaaggaatga cttcttcttt tttttcggga actgtttcct ttttagattc aacctcttgc
     4501 gcactgagag ataaaattga aagtaaaaag aaaataatta aataaatttt atacattgtt
     4561 taagatattt ccttaaagat tttttggaca aaaattgaaa tttgagagat gtccagactt
     4621 ttatagggaa aagttatttt tttaaatgtt agacttataa gttgactaac ggtaaaaaaa
     4681 aaattaattc ttcaaatgta gagcctttaa gaagaaaaaa ctatcaaaga gatcgttaac
     4741 tgaaaaggca cgttattttt ttatagtaac ggatattctt actctagttt cattctagaa
     4801 tttggatttg gaaaaagttt aacttttgcg tgtttagtag gggctgattt gaattctaat
     4861 tcctaaaaga tatgaaagta tatctactat cgcttatttc aaaccttgca tatccttaaa
     4921 aaccttggat tcattgatac actgtatcag tcttggcgac aaggggaaga gtgaattcaa
     4981 gactggctct gtttcgctga aggaactact ttctagatgt gaatcaagaa tttttctgaa
     5041 atactagtag ggattcctag ataagattct tttatttttc agagaatttg atcttgattt
     5101 attggggagt aatctgaatt tctccaactt ctagcgtgaa tttgattgca ttggaaatta
     5161 aatttcgtaa aacagcttga accttaaatc tatgcgcttt tatttagagc ttaggtggta
     5221 taggattatt gattaaacta ccacaggaaa aagaagcgga tttcctgaac tccgaattcc
     5281 tcagagtatt aaatacattg cttgtaagcg tgatgctttt tacctatagg cataatttct
     5341 aagactttct taagaggaaa taaaataaga gtgaactcaa gaacagataa aaactggcta
     5401 aggaggttat gatttttata gtcatagttt tcgcgagtgt gagtgtcgaa accatcaaa
//
LOCUS       DAOQOS010000225         5395 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_9220_length_5395_cov_5.663296, whole genome shotgun sequence.
ACCESSION   DAOQOS010000225 DAOQOS010000000
VERSION     DAOQOS010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5395)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5395)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5395
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9220_length_5395_cov_5.663296"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1771
                     /gene="pheT"
                     /locus_tag="PLF66_25485"
     CDS             <1..1771
                     /gene="pheT"
                     /locus_tag="PLF66_25485"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HMZ67467.1"
                     /translation="IVVENQNAHSYFASSVKGFKVQESIPKIKERLIRCGIKSINNVV
                     DISNYVMLEMGQPTHFFDKAKLGNVSLEVSFAKEKEKMALLDKTTKELDGNILIIRNS
                     GTPVAMAGVMGGEESAISNSTEEAVLESAVFKREDVRKTIRNTGIRTESAVRYEKGLD
                     SHSSLPVISRSLILLKENGCPNLSASKPSGFEHKKDRVEIKTSFTFINEKLGTIFEQK
                     VIVDILERLGFQTKVTKDELSVIVPVYRHNYDITIEEDLVEEVGRSLGYAAIVRKPLL
                     SDVKPVAMNPMRSVERVLKSIFTQNLGYSEVFNYSFASEEDTKFEKEENALGIANSMP
                     DEFKYLRTSIYPSILKHLSLNADRFESVKLFEYGRTYHKRNPNELAEEDRFFGFGVLK
                     NRKSNEFTEMENDFLSVRNDVDTLLKLFGFKNIQLEKTSLSYFHPNACVHWKVNGIVI
                     AELGYLHPKFNDLYNLKKRAIVGKLFFDSILKLNVPENKKFTFVPPSQFPQDQVDISL
                     VMEREATTEKYAELVSKLGVSEIQSIWVHDIYTGQNISQDKKSVTYRVSLLNYKETFT
                     QNAIKEITDKLIGLAKENGYQLR"
     gene            1772..2434
                     /locus_tag="PLF66_25490"
     CDS             1772..2434
                     /locus_tag="PLF66_25490"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HMZ67468.1"
                     /translation="MKCFILAAGFGKRMGDLTTNLPKPLLPYKNKTLLDHCIDFARNL
                     GITEFIVNTHYHADKIHKHLDRYTDLKIHISYEKEILGTGGGIKKGITGIIPKNEIFL
                     TLNPDVMVTGISSEESEAIRSYTGDCLLFLSNPEEGEKYTTLDLDKGKVYFREGDCFY
                     VGMSLLKASILDSIPENSFYDLALVFRKLSEENRLDGIKLTHSVIDFGDKEKYLKLTQ
                     SK"
     gene            2494..2937
                     /locus_tag="PLF66_25495"
     CDS             2494..2937
                     /locus_tag="PLF66_25495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67469.1"
                     /translation="MNRKKQKSIKKKHVEEIVRRKLDSQGYKMNHHREEVGIQPDIIA
                     EKDNTILYVEVAGYKTSPRAASRDFYEVFFRAFAQLEQIDSDNIVIALPIQYLHDMKK
                     RIESMKNGWVRLCIGFPEISVYFVDIKNDKIYKKRLPEILHTEEV"
     gene            complement(3128..4003)
                     /locus_tag="PLF66_25500"
     CDS             complement(3128..4003)
                     /locus_tag="PLF66_25500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004464703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HMZ67470.1"
                     /translation="MKFIHISDIHLPIKVPIFSLRGKMISGYLNYSLRRRKLHPVAVI
                     ESLLSFIQKTDYDCLILSGDITNVSHKLEFEEARKLLDPILNEKAFLIPGNHDRYMES
                     SVNPVDLYEKYFGEFSGDKIPNSEGEYIRIKKIKDLTLIGWDSNAPTPIAIATGFVKP
                     KVIEITKKYLTENKIKQYALVCHHPIWNPPDGFESEYHKMKNREEVVSLIQDFPPIAY
                     FHGHCHSNWIRNKTTNSPYHIINSASTTRLGSHTHKTGFHVGELQKDSLQIKRYAFDK
                     SEQSFKEDSLVWYEK"
     gene            complement(4000..4254)
                     /locus_tag="PLF66_25505"
     CDS             complement(4000..4254)
                     /locus_tag="PLF66_25505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002729955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl domain-containing protein"
                     /protein_id="HMZ67471.1"
                     /translation="MGTKNLFELKTPDLGDTDKIELIKWYSEVGQSIEEGDEILELVT
                     DKAAFPVESPVSGVLLEIKVPEGSIVQKDQLLGIMELIQK"
     gene            4354..5190
                     /locus_tag="PLF66_25510"
     CDS             4354..5190
                     /locus_tag="PLF66_25510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020768044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67472.1"
                     /translation="MANQKKFLNSKEISANLDEIIKRNYIHLELKGLTRDTRDLISAI
                     INGLLERIGADPLSSFHLFSGLMEALLNAIKGNIRFTIFKNELANKLKDVENSDKEID
                     RLLQIILDTSALRDAMQRYVVPEKIKKLVQNILSLEDKIRVKKVDISEQDRDFLYTIR
                     EKMKSDNRKISMKIRLDEKELYIRIKNDSPIMEGDMDRINESRQKHYQLYLEGRSSDY
                     FRPEFLDEKESAGFGIAMIDEGFYNMGLNPIEQFTYTQGKTTTTVYMIYPLDKLRAGA
                     FG"
     gene            5201..>5395
                     /locus_tag="PLF66_25515"
     CDS             5201..>5395
                     /locus_tag="PLF66_25515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67473.1"
                     /translation="MFSRKSFYFLFVFLLYIQVLYPFEILKNLEYENEVLQKLRSEVK
                     HNLKVSKSKGSDLEYIPLAFY"
BASE COUNT         1862 a          837 c          963 g         1733 t
ORIGIN      
        1 tattgttgtg gaaaatcaaa atgcacattc ttattttgct tcttctgtaa aaggatttaa
       61 agtccaagaa tcaattccta aaatcaaaga gagactgatt cgctgtggaa tcaaatcgat
      121 caacaatgta gtggatattt ccaattatgt tatgcttgag atgggtcaac ctactcattt
      181 ttttgataag gcaaaattag gaaatgtatc tcttgaagtt tcttttgcaa aagaaaaaga
      241 aaaaatggct ctcttagata aaactacaaa agagctagat ggaaatattt taatcattcg
      301 aaacagtgga acaccggtcg cgatggctgg agttatggga ggagaagaat ctgcgattag
      361 caattctaca gaagaagcgg ttttagaatc agcagtcttt aaaagagaag atgttcgtaa
      421 aaccattcgc aatactggga ttcgaactga atccgctgta agatatgaaa aaggtttaga
      481 ttctcattcg agtttacccg tcattagccg ctctctcatt ttactaaaag aaaatggatg
      541 tccaaatcta tctgcatcta aaccatccgg ctttgaacat aaaaaagaca gagtggaaat
      601 caaaactagt tttacattta tcaatgaaaa attaggaact atttttgaac aaaaagtaat
      661 tgtagatatt ttagaaagac ttggatttca aaccaaagtc accaaagatg agctaagtgt
      721 aatcgttccc gtctatcgtc acaattatga tattacaatt gaagaagatt tggtagagga
      781 ggttggtcgt tctcttggct atgcggctat tgtgcgtaaa ccgcttctat cggatgtaaa
      841 accagttgcc atgaatccaa tgcgatcggt cgagagagtt ttaaaatcga tctttactca
      901 aaatcttggt tattcagaag tttttaatta ttcttttgca tccgaagaag atacaaaatt
      961 tgaaaaagaa gaaaatgctt tgggcattgc gaattctatg ccggatgaat ttaagtattt
     1021 aagaacttcc atctatccat ctatcttaaa acatttatct ttaaatgcag atcgttttga
     1081 atctgtaaaa ctatttgaat atggaagaac ctatcacaag agaaacccaa atgagttagc
     1141 agaagaagac agattcttcg gatttggagt tttaaaaaac cgtaagtcca atgagtttac
     1201 agaaatggaa aacgattttc tttccgttcg aaatgatgtt gatactttat tgaaactgtt
     1261 tggttttaag aatatccagc tagaaaaaac aagtctctcc tatttccatc caaacgcttg
     1321 tgtgcattgg aaggtaaatg ggattgttat tgcggaacta ggatatctac atccaaaatt
     1381 caatgatctc tataatttaa aaaagagagc gatagttgga aaacttttct ttgattccat
     1441 tttaaaattg aatgttccag agaataaaaa gtttaccttc gttcctcctt ctcaattccc
     1501 tcaagaccaa gtagatattt cccttgtgat ggaaagagaa gcaacaacag aaaaatatgc
     1561 tgaattagtg agtaaactcg gagtatctga aattcaatct atatgggtgc atgatattta
     1621 tacaggtcaa aacatttccc aagataaaaa atctgttacg tatcgagtat ctcttctcaa
     1681 ttacaaagag acatttaccc aaaatgcgat taaggaaatt acagataaat tgattggact
     1741 tgctaaggaa aatggttatc agctacgtta gatgaagtgt tttattcttg ctgctggatt
     1801 tggaaaaaga atgggagatt tgactactaa tctccctaaa ccgttacttc cttataaaaa
     1861 taagacactt ttggatcatt gtattgattt tgcccgtaat ttaggtataa cagaatttat
     1921 tgtaaacact cattaccatg cagacaagat tcataaacat ctagatcgtt atactgattt
     1981 aaaaattcat atttcttatg aaaaggaaat cttaggaacg ggcggaggaa tcaaaaaggg
     2041 gataacagga attatcccaa aaaatgaaat ttttctaact ctcaatccag atgtaatggt
     2101 gacaggtatt tcctctgaag aatctgaggc aattcgaagt tatacggggg attgcctttt
     2161 atttctatca aatccagaag aaggtgagaa atataccact ctagatctag acaaaggaaa
     2221 ggtttatttt agagagggag attgttttta tgtaggtatg agcttattaa aagcatccat
     2281 tctagattcg attccagaaa attcgtttta tgatttagct cttgttttta gaaaattaag
     2341 tgaagagaat cgtttagatg gaattaaact cactcattct gttattgatt ttggagataa
     2401 agaaaagtat ttaaaattaa cccaaagtaa ataaataaat tcttgcaaca aagcagaaat
     2461 gtgagaaact gtaagagatg ttggggcaat aaagtgaata gaaaaaagca aaaatcaatt
     2521 aagaaaaaac acgtagaaga aatcgttcgt cgtaaactag attcacaggg ttacaagatg
     2581 aaccatcata gagaagaagt aggaattcaa cccgacatca ttgcagaaaa agacaatact
     2641 attttatatg tcgaagtagc tggatataaa acatctcctc gcgctgcctc tagagatttt
     2701 tatgaagttt tttttagagc ctttgctcaa ttagaacaaa tagattcaga taacatcgtg
     2761 attgctctgc ctattcaata tctgcacgac atgaaaaaaa gaattgaaag catgaagaat
     2821 ggatgggttc ggctttgtat tggctttccc gaaatatccg tttactttgt ggatattaaa
     2881 aatgataaga tttacaaaaa gagactccct gagatcttac atacagaaga agtataagat
     2941 ttaaaaatca tttcttacag aacataaacc cgatctaagt ttttttcacc accaaactac
     3001 actcagacga ttaaatgtgt gatcaccgat cactgaacga taaaagatat gacggataat
     3061 tttgagttat cttttatggg tggtagtctc ctctttcagc aagcttacgt tagatactat
     3121 ctttctatta tttttcatac cagacgagtg agtcttcttt gaaactttgt tcggatttat
     3181 cgaaagcgta tcgttttatt tggagcgaat ctttttgtaa ctcgccaaca tgaaatccag
     3241 ttttatgagt atgacttcca agtctggttg tagaggcaga gtttatgatg tgatagggag
     3301 agttagttgt tttatttcgt atccagttgg aatgacaatg accatgaaag taggcgatag
     3361 gcggaaagtc ttggattaga ctcacaactt cttctctgtt tttcattttg tggtattcgg
     3421 attcgaaacc atcaggcgga ttccagatag gatgatgaca aactaacgca tactgtttaa
     3481 ttttattttc tgttaaatat ttttttgtaa tttcaatcac tttaggtttt acaaaaccag
     3541 ttgcaatcgc gattggtgtt ggagcatttg aatcccaacc aattaaagtt aagtctttga
     3601 tttttttgat tcgaatgtat tccccttctg aattaggaat tttgtctcca gaaaattctc
     3661 caaagtattt ttcgtataga tccactggat tgacggaact ttccatataa cggtcatgat
     3721 ttccaggaat taaaaaggct ttttcattta agattggatc gagtagcttt cttgcttctt
     3781 caaattctaa cttatgggaa acatttgtaa tgtcacccga taatatcaaa caatcgtaat
     3841 ctgttttctg tatgaaagaa agcagagatt caatgacagc aacaggatgt aattttcttc
     3901 ttctaagaga gtaattcaaa tatccactga tcatttttcc tctgagagaa aaaataggaa
     3961 ctttgattgg caagtggata tcggaaatat gaataaattt catttttgaa tcagttccat
     4021 gattcctaat aattggtctt tctgaacgat tgagccttct ggaactttaa tttctagaag
     4081 aactccgctt acaggagatt cgacaggaaa agctgcttta tctgtcacta gttctaagat
     4141 ttcatcccct tcttcgatac tttgacctac ttctgaatac catttaatca gttcaatttt
     4201 gtcggtgtca cctaaatcgg gagtttttaa ttcaaataaa ttttttgtgc ccataaatgt
     4261 aaatccttct tgcagtaatg gcgatactaa ataatgtata tgaaattctc atcttggatt
     4321 tgataataaa accaatttaa agaacactag gatatggcaa atcagaaaaa attcttaaat
     4381 agcaaagaga tttcagcaaa tttagatgaa atcatcaaaa gaaactacat tcatttggaa
     4441 ctgaaaggac tcactcggga cactagagac ttgatttcag ccattattaa tggtttattg
     4501 gagagaatcg gggcagatcc tctgtcctct tttcatttat tcagtggtct tatggaagcc
     4561 cttttaaatg ccattaaagg aaatattcgt tttaccattt ttaaaaatga gttagcgaat
     4621 aagctaaaag atgttgagaa ttctgacaaa gaaatagaca gacttttaca aatcatccta
     4681 gatacaagcg cacttagaga tgcgatgcaa cgttatgttg ttcctgagaa aattaagaaa
     4741 ctagttcaaa atattttatc tttagaagat aaaattcgtg tcaagaaagt agatatctct
     4801 gaacaagatc gcgattttct ctatacaatt cgagaaaaga tgaaatcaga caatcgaaag
     4861 atttctatga aaattcgtct agatgaaaaa gaactttata tccgaattaa aaacgattct
     4921 ccaattatgg aaggagatat ggatcgtatc aacgaatcca gacaaaagca ctaccaactt
     4981 tatttagaag gtagatcatc cgattatttt agacctgagt tcttagatga aaaagaatca
     5041 gctggattcg ggattgcaat gattgatgaa ggcttttata atatgggtct caatccaata
     5101 gagcaattta cttacacaca aggaaaaacc actacaacag tatatatgat ttatccgctc
     5161 gataaactac gagctggtgc atttggttga ttcttagttt gtgttctcgc gtaaaagttt
     5221 ctatttttta ttcgtctttt tattatacat tcaagtttta tatccatttg aaattctaaa
     5281 aaatttagaa tatgaaaatg aggtattaca aaaactcaga agtgaagtaa aacataattt
     5341 aaaagtatcc aagtccaaag gttctgatct agaatacatt cctcttgctt tttat
//
LOCUS       DAOQOS010000226         5386 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_9234_length_5386_cov_5.238229, whole genome shotgun sequence.
ACCESSION   DAOQOS010000226 DAOQOS010000000
VERSION     DAOQOS010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5386)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5386)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5386
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9234_length_5386_cov_5.238229"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(148..900)
                     /locus_tag="PLF66_25520"
     CDS             complement(148..900)
                     /locus_tag="PLF66_25520"
                     /inference="COORDINATES: protein motif:HMM:TIGR01488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family phosphatase"
                     /protein_id="HMZ67474.1"
                     /translation="MKNPGVAAFDFDNTLIKNDFGEAVMNQLLLDGLENLKVPFEIYF
                     KDKEKAKDIWADRKTNPNRFKDFVWEEYSSIIQSQGVEKGYRWSSFIFSGWKEDEFRS
                     YARSVWKEELKREVKVGVSPYEEMRDLSHCLVENNWNVFIVTASPEEVIQEVSQEFLI
                     PKENVIGMRLEQENGFSTPRIIEPYTYGEGKVRALFQRKGLTADLAFGDSENDYPLLK
                     TAKRLALLIDKGKKELVEKCEEIHCLIQPMFK"
     gene            complement(983..1747)
                     /locus_tag="PLF66_25525"
     CDS             complement(983..1747)
                     /locus_tag="PLF66_25525"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011669809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HMZ67475.1"
                     /translation="MRKRYIIIFLMGLLLWSKVSTIANSNKKALKLDNEFIHSLGDKQ
                     GRWIIPRESAVSVSAYLKEIGSSEEEFRSINQLSATEPIPANRPLFFPFSEKYSRDLL
                     IQGKGREIIQSDYRELIWPVGTSNSKISSKLGQRRNLMHTGIDIACPNRSPVLAANDG
                     VVLSARNQGNYGLSLVIQHNINQIQTLYAHNSVLLVKEGDTVAKGQIIALSGSTGHST
                     GPHLHFEVRYQSVILNPEHYFPNETERSIALKEVDL"
     gene            complement(2536..2895)
                     /locus_tag="PLF66_25530"
     CDS             complement(2536..2895)
                     /locus_tag="PLF66_25530"
                     /inference="COORDINATES: protein motif:HMM:NF023276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3368 domain-containing protein"
                     /protein_id="HMZ67476.1"
                     /translation="MDGSKSQSDSLNKYLKNNTLDIKKESIIISDDSLDKGELSAMIL
                     YKQLNADFLLIDDRKGRRIPELNQISIIGSVGVLLLAKKKNLIPEIKSRIDIIIDSGI
                     FLSEEIIIKVLQASNEI"
     gene            complement(3008..3244)
                     /locus_tag="PLF66_25535"
     CDS             complement(3008..3244)
                     /locus_tag="PLF66_25535"
                     /inference="COORDINATES: protein motif:HMM:NF015630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0175 family protein"
                     /protein_id="HMZ67477.1"
                     /translation="MPDEFFFLEDKIALADEIRLSYALLLFKQVRISLMKAARLANKN
                     IYVFMNKCKKNKIPVIDFSEEEFEKELAIVNRLV"
     gene            complement(3825..4127)
                     /locus_tag="PLF66_25540"
     CDS             complement(3825..4127)
                     /locus_tag="PLF66_25540"
                     /inference="COORDINATES: protein motif:HMM:NF025120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HMZ67478.1"
                     /translation="MKKVKPIVTKDSKELAVALGFSSTVGLEFEIRSDLNDKIIEIVK
                     KQGLTHAQVAKLAQTSRTRITALMNRQSKDISIDLMLRILGSIGVHAKIQFKKNAA"
     gene            complement(4114..4446)
                     /locus_tag="PLF66_25545"
     CDS             complement(4114..4446)
                     /locus_tag="PLF66_25545"
                     /inference="COORDINATES: protein motif:HMM:NF017757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HMZ67479.1"
                     /translation="MKKVEFHPKALATLRDFPEAVRRELGQAIFELQKGNQLSMPFAK
                     PMPTVDTGVEELRVKDASGAFRIFYFTRLSDRVLIFHAFQKKTQKTPKHEIELGRKRF
                     KELYNEKS"
     gene            complement(4840..5298)
                     /locus_tag="PLF66_25550"
     CDS             complement(4840..5298)
                     /locus_tag="PLF66_25550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67480.1"
                     /translation="MNRALAQNSLVRQNNRLNALKQQENALIQDTTGSVIARAIGRED
                     AAGNLSQILGGLRKRQVQKRQNSQIGTVVSNGVANGMKMVNGIANGITRNAVIAFGGS
                     GENYDKLTATPFTPRATWTGAQKVDTSKQGMIDRMQEQALAKYLEANGMP"
BASE COUNT         1502 a         1121 c          907 g         1856 t
ORIGIN      
        1 atcgattccg tttaacttta atagcttgga tgtttctttg aattcttccc cgataatcag
       61 atcggcttgt tgcaacttct caattgcatt catagcaata tctcgatcat ttccaattgg
      121 aacagaaata atataaagag tgcccattta tttaaacatc ggctgaatca gacagtggat
      181 ttcctcacat ttttctacta attctttttt tcctttgtcg atgaggagag ccaacctctt
      241 agcagttttt aaaagtggat agtcgttttc agaatctcca aaggcaagat cagccgttag
      301 tccctttctc tggaataatg ctcttacttt tccctcacca taggtgtaag gttcaatgat
      361 tctaggagtt gaaaaaccat tttcctgttc taatcgcatt ccaatgacat tttcttttgg
      421 aattaaaaat tcctgactga cttcttgtat aacttcttcg ggagaagccg ttacgataaa
      481 tacgttccag ttgttttcca ctaggcagtg agataaatct ctcatttctt cgtaaggact
      541 tactcctacc tttacttctc ttttaagttc ttctttccat acagatcttg catagcttct
      601 aaactcatct tctttccaac cagaaaaaat aaaagaagac catcggtatc ccttctctac
      661 cccttgggac tgaataatag aagaatactc ttcccagaca aagtctttaa accgatttgg
      721 atttgtcttt ctatccgccc aaatatcctt tgccttttct ttgtctttaa aatagatttc
      781 aaacggaact tttaggtttt cgagaccatc tagcaaaagc tgattcatca cagcttcgcc
      841 aaagtcattt ttaatgagtg tattatcaaa atcaaaggca gcaactcctg gatttttgat
      901 ttcttccaga agtcgcttat ggttgtcgga actccaacct ctctttggaa attcagttag
      961 cttgctaatg ttagatttcc ttttatagat ctacttcttt tagggcaatc gatctttccg
     1021 tttcatttgg gaagtaatgt tctgggttta aaataacact ttggtatctg acctcaaaat
     1081 gcaaatgagg tcctgttgaa tgaccagtac ttccagaaag ggcaatgatt tgaccttttg
     1141 caactgtatc tccctctttg actaggagca cagagttgtg ggcatagaga gtttggattt
     1201 ggttgatatt atgttgaatc acaagagaca atccatagtt cccttggttt cgagcggaga
     1261 gaaccactcc atcattagcg gcaagaacgg gagatcggtt tggacaagca atgtcaatcc
     1321 cagtatgcat taaattcctt ctttgtccta atttagaaga gattttggaa ttagaagttc
     1381 caacaggcca aattaattct cgatagtcag actggataat ctctctgcct ttcccttgga
     1441 tcaataaatc tctgctgtat ttttcactga acggaaaaaa taggggacgg ttcgcaggaa
     1501 ttggctcagt agcactaagt tgattgatag atctaaattc ttcttcacta gaaccgatct
     1561 cttttaagta ggcagataca ctcactgccg attctcgagg aataatccat cttccttgtt
     1621 tgtccccaag actatgaatg aactcattgt ctagttttag ggctttctta tttgaatttg
     1681 ctattgtcga aaccttcgac caaagcaaga gtcccattag gaatatgatt atatatctct
     1741 ttcgcatttt tgcatccctc aatcaggtta attttcgcta aatttttatc ttacgtatac
     1801 ggtaatttgt acaaggctct cctgtcaagg tttttagagc cctaaaaact gcctaatgag
     1861 acatagaatt ataagataga atcgatctta ggcagattcc aaaaagagag ctttcctact
     1921 cagggaagaa aactccgact tttctaaaac gaatcttatt tttgggaatt ggaaatcctc
     1981 aaactcttcc aaaatgggaa tttagttttg gtctgaggaa aaaaaagcaa caaggattaa
     2041 gtggaaagaa tgaagagctg ttccaattca aggtttattt ccaaactatt cgtttgacac
     2101 tcgatttctt agccatacat cattccatgg aagttctggt atttagattt gatcgaatct
     2161 gaaaagctat ttgcttcttc tccaatgatt tcaaaatttc cacctactgc atctctaacc
     2221 aattttagaa ttctattggg cgaatcggtg gtattcacag aaagaggtct tgataaatca
     2281 agaccctaca ttttttcaca acgcaccgac cgcgctctcc cttcaacgcg gttttcggtc
     2341 gatcgctttc gctagagtag ggtaactttt gtttgccggc gtttgatttt ttttacaaac
     2401 atcaatggtt acatcctgta taccgttggc attcctcctc ccagtaaatc attaatcaaa
     2461 gaaggtaact actgttcaca tcattcggat ttcgtttggc gagctacatc catatataat
     2521 caggtagggt ttagatcaaa tttcatttga ggcttgcaag acttttatga taatttcctc
     2581 tgataaaaaa attcctgaat ctatgattat atcaattcgt gatttaattt cgggaattaa
     2641 atttttcttt ttagcaagca gtagtactcc aacgcttcca atgatactaa tttggtttag
     2701 ttcaggaatt cgccttcctt tcctgtcatc tatcaaaaga aaatctgcat ttagctgctt
     2761 atataaaatc atagcggata attcaccttt atctaatgaa tcatctgaaa taattatact
     2821 ttcttttttt atatctaaag tattgttttt aagatatttg tttagagagt cactttggga
     2881 tttactacca tccatgatct catagtcaac gtctgtggga actttaacct cgccaaaagt
     2941 ttatccaaaa gaaaaagttc atcacaataa gacaatgcaa tgagagcaga agaatctgca
     3001 actagaatca taccagacga ttcactatag caagctcttt ctcaaactcc tcttcgctaa
     3061 aatcaattac tggaattttg ttttttttgc atttattcat aaatacgtaa atatttttat
     3121 tggctaagcg agcagctttc attaaagaaa ttctgacctg cttaaataac agtaaagcat
     3181 aactcaatct tatttcatct gctaaagcaa tcttgtcttc taaaaagaaa aattcatcag
     3241 gaatttctat atttaaaatt ataattttat actacaaagc caatttaaaa attacaggaa
     3301 aaaaaagtcc taaaactgca ctttatcttt ctaacgctat cgctaattcc catccatctt
     3361 tttcaaatat tctctcccgt ttctatctta caattgaatg tcttaagcaa aaaaggattg
     3421 tgagtctttt ataatgaggt gttttgaaat aaactaggta acatccccct gcgcattacc
     3481 accattagcc cctgatgatg gagaactggt tccgcctcct gctatgagtc ttgctccatt
     3541 tctatctaca ttcattcctt gtctctttct tctgggcgtt acgcggttac tttgtattag
     3601 ctgtatattt atttctttgt ttatttttta ctacaatcgc gtcgggctct ccctcttgtc
     3661 atcttttttc acacttcgat acaatgctaa gaagcatcta ctcagtgacc caaaaaaaga
     3721 tgacaccgct cgatccctaa cgctagaagt tgggagactt tgttgattga ttgggtaatc
     3781 attgttagcc cctgattgag ttttgttacg gcgagggaca acacttaggc tgcatttttt
     3841 ttgaattgaa tttttgcatg aacaccaata gacccaagaa ttctgagcat caaatctatg
     3901 gatatatctt tcgactgacg gttcataaga gcagtaattc ttgtccttga cgtttgagca
     3961 agtttagcaa cttgggcatg agtaagaccc tgctttttta cgatctcgat gattttatca
     4021 ttcaagtcac tgcgaatttc aaattctaaa ccaacagttg agctaaatcc aagagcgaca
     4081 gcaagctcct tcgaatcttt tgttacaata ggcttaactt ttttcattat ataactcctt
     4141 aaatctttta cgacccaatt caatttcatg ctttggcgtt ttctgtgttt tcttttgaaa
     4201 tgcatgaaaa attaatactc tatcagaaag tcttgtaaaa taaaaaatcc gaaatgcacc
     4261 gcttgcatct ttcaccctta attcttctac tcccgtatcg acagttggca taggcttagc
     4321 aaagggcatt gagagctgat tacccttttg taattcgaaa atcgcctgac ccaattctcg
     4381 acgcactgct tccggaaaat ctctaagtgt tgcaagtgct ttgggatgga attcaacttt
     4441 cttcactatt tcaatgtctc atatctgaga cactttgaca agaaaaaatt cttttcctaa
     4501 atgatttcgt tctttcgccg tggcagaatg gagttatcga acgttttaag ggaactgttc
     4561 gaagggagtt attcgatcat gttattccgt cgaagctccg ttagccatta ggcggtagga
     4621 gctgaggcct gctagacatt ttctgggaaa tgaaaatggg ggcggaatgc agatcatcgt
     4681 gattcccttc tcaaagatta cgttcactat tacaataata atcatacata tttatttaga
     4741 aataaagatt ctcctctttg tagagtctat gcatctctcc cttgcaaact ttactcacct
     4801 gattcaattc cagtgttagg aggactacgc catgtatact catggcattc cattggcttc
     4861 taaatacttt gccaatgctt gttcttgcat tctatctatc attccttgtt tcgatgtatc
     4921 tactttctga gctcctgtcc aagttgctct cggtgtaaat ggggttgctg tgagcttatc
     4981 gtaattctct ccacttcctc caaatgcgat cactgcattt cgagtgattc cattggctat
     5041 accattcacc atcttcatcc cattcgctac tccattcgat accactgtgc ctatttgcga
     5101 attctgtcgt ttctgaactt gtctctttcg aagtccccct aggatctgtg atagatttcc
     5161 ggctgcatcc tctctgccta tcgcacgtgc tatcacactg ccggttgtat cttggattaa
     5221 agcgttctct tgttgtttca aggcattcaa tcggttgttt tgacgaacaa gggaattttg
     5281 agcgagtgct cgattcacca tcgctcctcc aatcggtcca cctatcgctg atctgaacta
     5341 ctacatattt agtggacacg attttaggca aattgcttct catatt
//
LOCUS       DAOQOS010000227         5368 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_9286_length_5368_cov_6.076793, whole genome shotgun sequence.
ACCESSION   DAOQOS010000227 DAOQOS010000000
VERSION     DAOQOS010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5368)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5368)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5368
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9286_length_5368_cov_6.076793"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..926)
                     /locus_tag="PLF66_25555"
     CDS             complement(<1..926)
                     /locus_tag="PLF66_25555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67481.1"
                     /translation="MLDFSLFDFSILGFGGWFCLVWAAGLSLQISDKTKLVAVYFVGT
                     SGFRLIWESFVLSDLIFLLPEMYCIPVPFLYLIGPSILLYYERLGGTEKWKLSSYHFL
                     PFVLGFLPFILWFNFDSEQKKQIIHSVLSGNWSFPDSLFLVWIIGPKISILIYSLFIS
                     YRKSGEGVLAIKLLPEKIQFFSIILLIYILIMILADIAGYIIGQKFLYRYSAWSHTIA
                     AIIVYLYSKLNPYSMLEISGAIKTARYSQSKLTSVNSIEAVQKLSFLMSNESYYADED
                     LRLSTLAEAMKMSSHQLSELINVHFQMSFIHFV"
     gene            complement(1260..1778)
                     /locus_tag="PLF66_25560"
     CDS             complement(1260..1778)
                     /locus_tag="PLF66_25560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67482.1"
                     /translation="MKLTHIIRYIVGILILVNCAVPATREGMTYKNFEPRKKIKESIY
                     IDQSVGGSETNPFLVSKIANEEFTNAIQDSIKESDLFHVVDEKKENVWLLKSEIINQD
                     QPYFSFSTNCKVLVKYSLYKSNTLVKEFQIEETASASLSDSLRPIKRVQLANEKAGRA
                     NINKLLQELEKI"
     gene            complement(1853..3661)
                     /locus_tag="PLF66_25565"
     CDS             complement(1853..3661)
                     /locus_tag="PLF66_25565"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HMZ67483.1"
                     /translation="MKNRILNSSIHISINSVLLILNLFLFNCNSLPDKPESIPKDDYS
                     FFREYLNRYIPKKMKEASIVGLSVSAVTDKEVLFAKGFGYADEGNGKEATADTIFRTA
                     SVSKIINLIITMKLVEEGKLNLDKDISQYLPELKLKSRFPNSKPITIRSILTHHSGLP
                     SDRMKGFFSYDKTDSLEKLISETSGEYVSYPPEFIYSYSNLGHSILGRIIEKVSGKTY
                     AKVINEKIFQPFGMKNSFYEVVPEKKNDFAKGYGGIILKSEVNEPYLRDLPAGFLHSS
                     VNDFSKVIQMFLNDGKINGSTYLKESTLNEIYTIQNPNNAYDDDLKIGLSFFVNTFDL
                     GKDIFNVSHGGDTFLYHAILGILPKEKIGVIVLSNTDVSAPVINDIAQKTLEISLETK
                     TGYKKPMNLNLTKAANVDMSSYAGTYQNGAMLRVEVENNNPFAKIDGGIQYILPNKEW
                     EWQDGKLKIFGLFTVNPPMQFKFKTVGKDKLLYLKVGGNVLLWGSKITPQESIDGSWL
                     KRLGKYKILNEDKNNAGMIKDPELKMENGFLVLETNGIPAANANVKQKLALQVLNEKE
                     SIIAGLGRSKADTISVKEVNGKEILTYSGYEMEKME"
     gene            3811..4086
                     /locus_tag="PLF66_25570"
     CDS             3811..4086
                     /locus_tag="PLF66_25570"
                     /inference="COORDINATES: protein motif:HMM:NF033789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autorepressor SdpR family transcription factor"
                     /protein_id="HMZ67484.1"
                     /translation="MDILFKALNDKTRREILEMLKEKDLTAGEISEQFNMSKPTISHH
                     LDLLKQASLVVSIKQGQHIYYSINSTELDELLKWILKVKDKVKGKKK"
     gene            4083..4733
                     /locus_tag="PLF66_25575"
     CDS             4083..4733
                     /locus_tag="PLF66_25575"
                     /inference="COORDINATES: protein motif:HMM:NF025015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HMZ67485.1"
                     /translation="MKQIIKREFLFWILLCSQFLFLFYFQDRIPSELPTHWDFSGNPD
                     SYSNKYTFPIINTVIYFVLLLIPKLDPRKENYKLFSDSFIKIRFLLTFIFTLLFYGIC
                     LRYFGFQIPEDKMIFIVVLSIFTAIGNYMRNFRPNYFVGIRTPWTLENETVWKKTHEL
                     GGKLFFYNGLIGILICIILDGMILPFLVLGLILIACLIPIFYSYFYYKKLKSNSKS"
     gene            complement(4851..5216)
                     /locus_tag="PLF66_25580"
     CDS             complement(4851..5216)
                     /locus_tag="PLF66_25580"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HMZ67486.1"
                     /translation="MKVGYVVLYVQDEKQSLEFWTKKVGMIVKKSQEAAGFQITRVGF
                     KDQDFSFELVPLKLMENNPDGLNLGIPSICFTAPDLVALHTQLKSNGVKVTDIANHFG
                     TDSFAFSDSEDHYFAAIQE"
BASE COUNT         1725 a          878 c          815 g         1950 t
ORIGIN      
        1 acaaaatgaa tgaaactcat ttgaaaatga acattgatca attcggataa ttgatgagaa
       61 ctcattttca ttgcttcggc gagtgtagac agtcttaaat cctcatctgc ataataggat
      121 tcatttgaca tcagaaaact aagtttctgc actgcttcaa ttgagttaac acttgtcaat
      181 ttggattgtg aatagcgggc tgtttttata gcacctgaaa tttccaacat cgaatacgga
      241 tttaattttg agtataaata tacaataata gcggctatcg tatgagacca agcactgtat
      301 ctataaagaa atttttgtcc aatgatataa cctgcaatat ctgcaagtat catgattaaa
      361 atatagatga gtaatattat cgagaaaaat tgaatctttt caggtaatag tttgattgct
      421 aaaactcctt cacctgattt tctatatgag ataaacaaac tatagattaa aatagaaatc
      481 ttcggaccaa taatccatac tagaaataga ctgtcgggaa aactccaatt cccacttaaa
      541 acagagtgaa tgatttgttt tttttgttct gaatcaaaat taaaccacaa aatgaaaggt
      601 agaaatccaa gcacaaatgg tagaaaatgg taactggata atttccattt ctctgttccg
      661 cctaaccttt cgtaatagag taaaatggaa ggaccgatta aatataaaaa gggaacggga
      721 atacaataca tttctggaag cagaaagatt aaatcgctta aaacaaagga ctcccaaatt
      781 aagcgaaatc cagaagttcc cacaaagtag accgcgacta attttgtttt atccgagatt
      841 tgtagagaaa gacctgctgc ccaaaccaag cagaaccaac ctccaaatcc aaggatacta
      901 aaatcaaata atgaaaaatc tagcaaattg agtatccttt tccaaaatcg aaggattgag
      961 tcttgaggtc gctattaaat gaaagcgatt tctgaatctg aattgaattt aacttgaatg
     1021 gtgttctatt tgaattcatt tgattgaaag ccttacagtc cttggactag actagttgca
     1081 aaaaaagaaa aaattataaa tagaaatgtt tgtttccttt tcttgaaaaa ctccctctac
     1141 aagaaaaatc gaatagtcta gtgctttatg ctttttattt tgatttgatc taaaatgaaa
     1201 tagatagatc agttcagctt ttctaaaaac tgaactgatc tcttagttgc catgagaatt
     1261 tatatttttt ctaactcttg taggagctta ttgatattag cccttcctgc tttttcgttt
     1321 gccaattgga cacgcttgat tggtcttagt gaatcactga gtgaagcact agctgtttct
     1381 tcaatctgaa attcttttac aagggtattc gatttataga gtgagtattt cactaaaact
     1441 ttacagtttg tgctaaagct aaaatagggt tgatcttggt tgattatttc tgattttaat
     1501 aaccaaacat tctctttttt ttcatctact acatgaaaca aatcagattc tttaattgaa
     1561 tcttggattg catttgtaaa ttcttcgttt gctattttcg aaactagaaa cgggtttgtt
     1621 tcacttcctc caacagattg atcgatgtag atactctctt ttattttttt tcttggttca
     1681 aaatttttgt aagtcatacc ttctctagtt gcaggaactg cacagtttac taaaatcaga
     1741 attccaacta tgtatcttat aatgtgtgtt agtttcattg ttatcctctt atgatattga
     1801 acgagtgtaa aaattttcgc atgacttctt ttttcgaatt catttttatg gactattcca
     1861 ttttctccat ttcataacct gaataggtta agatctcttt gccgttaact tctttgacag
     1921 aaatggtatc agccttactt cttccgagtc ctgcgattat agattctttt tcatttagaa
     1981 cctgaagggc aagtttttgc ttaacgttcg catttgctgc tggaattcca tttgtttcca
     2041 atactaagaa tccattttcc attttcagtt ctggatcttt tatcattcct gcattatttt
     2101 tatcttcatt taatatttta tattttccta aacgttttag ccaagagcca tcaattgatt
     2161 cttgaggagt gattttgcta ccccaaagta aaacatttcc cccaacttta agataaagca
     2221 atttgtcttt tccgactgtc ttaaatttga attgcattgg tgggttaacg gtaaatagcc
     2281 caaatatttt taattttcca tcttgccatt cccattcttt attaggcaag atatactgaa
     2341 tccctccatc tattttggca aaaggattgt tattttccac ttcaactcga agcattgctc
     2401 cgttttggta ggttcctgca taagacgaca tgtcaacgtt agctgccttt gtaagattta
     2461 gattcatggg ttttttataa ccagtttttg tttctagaga gatctccaag gttttttggg
     2521 caatatcatt tataacagga gctgagacgt ctgtatttga taatacaatc actcctatct
     2581 tttcttttgg aagaattcca agtattgcat ggtatagaaa agtatctcct ccatgcgaaa
     2641 cgttaaaaat atcttttcct agatcaaatg tgtttacaaa gaaacttaaa ccaattttta
     2701 aatcatcatc atatgcgtta tttggatttt ggattgtata aatttcattc aatgtggatt
     2761 cttttaaata ggtagaccca ttgatttttc catcattcaa aaacatttga attaccttag
     2821 aaaaatcatt cacagacgaa tgtaaaaatc ctgcgggtaa atcccttaaa tatggttcat
     2881 ttacttctga ttttaaaata attcctccat agccttttgc aaaatcattt ttcttttctg
     2941 gaacgacttc ataaaaagaa tttttcattc caaaaggttg gaagattttt tcatttataa
     3001 cttttgcata agttttgccg gaaacttttt caatgattcg tccaaggata gaatgtccga
     3061 ggtttgaata tgagtaaata aattctggtg gataactaac atattcgcct gacgtttctg
     3121 aaattaactt ctccaatgaa tctgttttat cataggagaa aaaccctttc attcgatcag
     3181 aaggcaatcc agaatgatga gttagtattg atctgattgt aattggtttt gaatttggaa
     3241 atctagattt tagttttaat tctggtagat attgcgaaat atctttatct agatttaatt
     3301 ttccctcttc aactaatttc atagtaatga tcagattaat gattttagag actgaggctg
     3361 ttctaaaaat tgtatcagca gttgcctctt ttccgtttcc ctcatctgca taaccaaaac
     3421 cttttgcgaa taacacttct ttgtcggtca ctgcacttac gcttaaacca acgatggaag
     3481 cctctttcat ttttttagga atgtatctgt ttaagtattc cctaaaaaaa gaatagtcat
     3541 ccttaggaat actttctggt ttgtcgggta gggaattgca attaaataga aataaattca
     3601 ggattaagag cactgaattt attgaaatat gtatgcttga atttaatatt ctgtttttca
     3661 taaaattacc tttgtttaga taattagaca attatctaaa tatctaaata ctgtcaatcc
     3721 ttaaaaattt ttaaaaagta gaaaatattt cttacaaaga aaaaatcgta tacaaaatta
     3781 aactgtttgt gagttattat ttcaggcgcg atggatattc tatttaaagc acttaacgat
     3841 aaaaccagaa gagaaatctt ggaaatgctc aaggaaaaag atttaactgc cggagaaatc
     3901 tccgaacagt ttaatatgtc aaaacctacc atttcccacc accttgattt attaaaacaa
     3961 gcgagtttgg ttgttagtat taaacaagga caacatattt attattccat aaatagcacg
     4021 gaactagatg agttgttaaa atggatttta aaagtaaaag acaaagtaaa aggtaagaaa
     4081 aaatgaaaca gatcatcaaa cgggaatttt tattttggat attactttgt tcccagtttt
     4141 tattcttatt ttactttcaa gataggattc cttcggaatt acctacacac tgggactttt
     4201 ctggaaatcc ggattcctat tcgaataaat atacttttcc cattataaat acagtgattt
     4261 attttgttct tttgcttatc ccaaaactag atcctagaaa ggaaaattat aaattatttt
     4321 ctgattcatt tataaaaata cgatttctac tgacgtttat atttacattg ctattttatg
     4381 gaatttgtct tcgctatttc ggatttcaaa ttcctgagga caaaatgatt tttatagtcg
     4441 ttctttctat ctttactgca ataggaaatt atatgcgaaa ttttcgtccc aattattttg
     4501 tcggaattcg cactccttgg actttggaaa atgaaactgt ttggaaaaag actcacgaac
     4561 tcggcggtaa gctctttttt tataatgggc taataggaat tctaatttgt attattttgg
     4621 atgggatgat tttacctttc cttgttcttg gtttaatttt aatagcctgc ctaattccaa
     4681 ttttctactc atacttttat tataaaaaat taaaatccaa ttcaaaatcc taggttggtt
     4741 taaccagaaa tgattccaat aatggttttt attatagaaa agtaagagaa ttgaatctgt
     4801 tcagcttttt tataaatcga taggctaaac agatacgata tgcgaattat ctattcttga
     4861 attgctgcga aataatgatc ttccgaatca ctgaaagcaa aggagtcagt gccaaaatga
     4921 tttgcgatgt ctgttacctt gactccattg cttttcaatt gagtatgaag agcgactaga
     4981 tcaggagcag taaagcaaat agaaggaata cctaaattga gtccgtctgg attgttttcc
     5041 attaatttca aaggaactag ctcaaaagaa aaatcttggt ctttaaatcc aactcgcgtg
     5101 atttgaaacc cagcagcttc ttgggatttt tttactatca ttcctacttt ttttgtccaa
     5161 aactccaaac tttgtttttc atcttgaaca tataaaacta cgtatcctac tttcattctt
     5221 attcctttga ttttattatt gtcgaatcta gttaatcaca aacaaccaat cgatttatat
     5281 aaaatcatta tctttccttt tctagtcaat tcattctaaa tttagaagag ttaatttgat
     5341 ttccctccta tttttaaata atatatgt
//
LOCUS       DAOQOS010000228         5357 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_9315_length_5357_cov_5.946624, whole genome shotgun sequence.
ACCESSION   DAOQOS010000228 DAOQOS010000000
VERSION     DAOQOS010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5357)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5357)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5357
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9315_length_5357_cov_5.946624"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            54..752
                     /locus_tag="PLF66_25585"
     CDS             54..752
                     /locus_tag="PLF66_25585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67487.1"
                     /translation="MIVKKIKKVIKSSEFTSDLKEYSNYAMKIKLERFIAFSFAKRFS
                     KSYDYETILEENDIDLIVRKDNENYKIELKYHFDFDILKIYNEMNKYKNLENIAKSIT
                     SKEKGLNWKVIPNILKDMQPKRFNKKTKKDSPDLFIWIIAKRDISKVTEDKFNSICMA
                     KQEIEYIEYLNSNKVKDYNEEEVLKAFSNNLMEFSIKTNKTNYLYESIKIKLDAILFP
                     IEYRFNIFYKNNLE"
     gene            932..1822
                     /locus_tag="PLF66_25590"
     CDS             932..1822
                     /locus_tag="PLF66_25590"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HMZ67488.1"
                     /translation="MSNPENEKKKIPIELIINILEESYNDLPEKNGNGTDYLDRRYRN
                     YREKLGEKPFFIKIDFQKKIIQNFKGNEENVKRLTKIYLENFAEDYSDYILDNYLKVQ
                     KENGLLEYSLFLIVFFNLASRLGLKVEMTYDNILDSSKKRMRRIRPIGLSCRRPYLNL
                     VAQDVNDKIYKHFVLSCITEIHTGIFDFAKDSERKRTNLDLKHFRNSKDYRFGRDYTT
                     YRIELNKKYLKHFRNVYFPEFETIEENTDTVILEVTTWDYSYFFNAIFNYREHCLLVA
                     PLDKVAFYKNILLNTLKNYS"
     gene            1916..2269
                     /locus_tag="PLF66_25595"
     CDS             1916..2269
                     /locus_tag="PLF66_25595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67489.1"
                     /translation="MRLNKILLLLSLFTSIGLYAVDGPTIKNMFNGNIKVQLLDSNEK
                     VLESQELSYYSSVTMIRCRSGNLLVVMWGEYEKDGRPKAVGSIMKDCSKRLLFTSDKN
                     IVLEKEMNLNKLVPK"
     gene            2967..4061
                     /locus_tag="PLF66_25600"
     CDS             2967..4061
                     /locus_tag="PLF66_25600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67490.1"
                     /translation="MDIIAKITGIEYKIYVREELKKFSLSEFDINALPASFILEDKNV
                     SFAISKWVSPKRTRSYPYERVYNTLSSTKKITIIPIVKDEGFEGDRDFIQWDTVSMMS
                     LLDVYVILAYYEKAEKHKSRKDKITNQSFSNDFILSKIKEIKNYHSSALHWNINELKI
                     NLYNLMDKVKHSYSQIETNLKTKLHSTKGLDDFQKQIKKDLDFFMDSSRKKSMEAQAR
                     EIETNQPKEFLKTFTKAKITITNYLGGKYFLTIDEIELEEKMLYLIESKHSKSSVLPS
                     LSDIKDGLLKLILLSNLKDVTVDGRKIKCKPILNLTSLKLKGSIQSDSTELDKFFKAN
                     AFNEKNKELIKKIFKEANENNFLVRIKRAK"
     gene            4062..4919
                     /locus_tag="PLF66_25605"
     CDS             4062..4919
                     /locus_tag="PLF66_25605"
                     /inference="COORDINATES: protein motif:HMM:NF014177.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HMZ67491.1"
                     /translation="MLIKSPLRYPGGKSRAIELISTLVPEFQEFREPFVGGGSVFIYL
                     KQKYPNKKFWINDLYFQLYTFWDMCQKDLSAVVKQIWSWRNEFENGKDLHKFLNENIS
                     KFNSVQIASAFFIYNRITFSGTSESGGYSEQAFRGRFTESSIVRLQNMNRILENLRIT
                     NLDFEEVVNEKGKNVFIFLDPPYYSATKSALYGKNGNMHKKFDHVRFAETMRNCKHKW
                     LITYDDCEHIRELFKFANVMSWNLTYGMRNVTDSSDQNGKELFISNYLEELPSIKQLD
                     LFSTKPVLV"
     gene            5107..>5357
                     /locus_tag="PLF66_25610"
     CDS             5107..>5357
                     /locus_tag="PLF66_25610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67492.1"
                     /translation="MEFISLFGKLSQVQCLHYCHETCRKEQVCAPSVQSQDLLVIRLA
                     RRHDFKIQKMLPHFRILKPRRLLLTLGDMPARILKNIAV"
BASE COUNT         1978 a          747 c          912 g         1720 t
ORIGIN      
        1 tactcttaac gttaggcggc attttaattt aaaggagtat attgggtaac agtatgattg
       61 taaaaaaaat aaaaaaagta attaagagtt cagaatttac atcagattta aaagaataca
      121 gtaattatgc aatgaaaata aaactagaaa gattcattgc attttcattt gctaagcgtt
      181 tttctaaatc atatgactat gaaactattc tggaagaaaa tgatattgat ttaattgtta
      241 ggaaagacaa cgaaaattat aagattgaac taaaatatca ttttgatttc gatatactga
      301 agatttacaa tgaaatgaat aaatataaaa atttagagaa tattgcaaaa tctattactt
      361 ccaaggagaa agggttaaat tggaaagtaa taccaaacat tttaaaagat atgcaaccca
      421 agagattcaa taaaaaaact aaaaaagatt cgccagattt atttatctgg ataatagcta
      481 aacgagatat ttcaaaggtt acagaagata aatttaatag tatttgtatg gctaaacaag
      541 aaattgaata tattgaatat ctgaactcaa ataaagtaaa agattataat gaagaggaag
      601 ttttaaaagc attttctaat aatctcatgg aattttcaat aaaaacaaac aagacaaatt
      661 atttatatga aagtattaaa ataaaattag atgctatact atttccaatt gaatataggt
      721 ttaatatttt ttataaaaat aatttagagt agtagtcatg taactttata caaaaacttt
      781 tagaaaattc aaaagccagg atttaaaaca taaacaaatg caccaagata tacatgatat
      841 tcatattcat taactcatga ctaaaacaaa tctttcagct caagaacaaa tcactcgagg
      901 catagagcaa cttacgcttg ggtggtattt catgagtaac cccgaaaacg aaaagaagaa
      961 aataccgatt gaattaatca taaatatatt agaagagtct tataacgatt taccggaaaa
     1021 aaatggaaat ggaacagatt atttagaccg cagatataga aattacagag agaagcttgg
     1081 agagaagcca ttctttataa aaatagattt ccaaaagaaa atcattcaaa acttcaaagg
     1141 aaatgaagag aatgtaaaaa gacttacaaa aatttattta gaaaactttg cagaggatta
     1201 tagcgattat attctagaca attatttaaa agttcaaaag gaaaatggac ttttagaata
     1261 ttctcttttc ctaatagtat ttttcaacct tgcatctagg ttaggattga aagttgagat
     1321 gacttacgat aatattttag attcatccaa gaaaagaatg agaagaatac gacctattgg
     1381 actttcatgc cgccgtcctt atcttaactt ggtcgcacaa gatgtaaacg ataaaattta
     1441 taaacatttt gtattgagtt gcataacaga aattcatact ggcatttttg attttgcaaa
     1501 agattcagaa agaaaaagga cgaacttgga tttgaaacat tttcggaatt caaaagatta
     1561 tcgcttcggg cgagattaca caacatatag aatcgaatta aataaaaaat atctcaagca
     1621 ttttcgcaat gtctactttc ctgaatttga aacgattgaa gagaatacag acactgtaat
     1681 tttggaagtt acgacttggg actatagtta tttttttaac gccattttta attacagaga
     1741 gcattgtttg ctggtagccc ctcttgataa agttgcattt tacaaaaata ttcttcttaa
     1801 tactttaaaa aactattcat aagactaaaa aagttttgag cggaaaaact ttttcctctt
     1861 gaaactttaa gattgttgta tacttaattt ctattttatg aattaaggaa tttctatgag
     1921 attaaacaaa attttacttc tcctctcact ttttacatcc attggtttgt atgctgtcga
     1981 tggacctacg attaaaaata tgttcaatgg aaatatcaag gtgcaacttt tagatagtaa
     2041 cgaaaaagtg ctcgaaagtc aagaacttag ctattactct tctgttacaa tgataagatg
     2101 tcgttcaggt aatctacttg tagttatgtg gggcgagtat gaaaaagatg gaagaccaaa
     2161 agctgttggt tcaattatga aagattgtag taaaagatta ctttttacat cagataaaaa
     2221 tatagttcta gaaaaagaaa tgaatcttaa taaacttgta ccaaaatgaa cgcagaataa
     2281 gcgtctgatt tttttttcga atttgctttt ctaccagttg cataaactgg aatcatttac
     2341 cgccttcgcc atccgtggct caggcggttt tagtatatgt gcgtcaattt tttgcttttt
     2401 aggagtagag aaattaaaac gccgcctaac aaagagtgag ccactgcgaa gccacgagaa
     2461 gatgtggctt cttgctccga gcctactttg tagtggaagt gaaaattttt agtttctttg
     2521 tgagcctaat gcaagttacg cgccttcgtt tcaggtacaa aacttgcgga agaaaagagc
     2581 aagttttgtg cctttcactc agtcacgcaa cttgctagta atagtgtggc tcggagacat
     2641 gactttaaaa ttcaaaaggt gctaccgcac tttcgaattt taaagtcacg tcggctactc
     2701 ttaacgttag gcgacatttt attttcgacc acgcttttga atgaactgac tttaaacttt
     2761 ttagaagtag gtttcctcga cctcgtgtct cggaaagact ttaagatttc tttccaaatt
     2821 taaagcgttg atctttttaa ataaagaaat atagtttctg tttagttcgt attgattttg
     2881 aaaatagggt tttttatata aaaacaagaa atcaagagac atatttttat tgcataaata
     2941 agccttttct agaagtattt atctaaatgg acataattgc aaaaataact ggcattgagt
     3001 ataaaattta tgtaagggaa gaactaaaaa aatttagttt atctgaattt gacattaatg
     3061 cattaccagc tagtttcatc ttagaagata aaaatgtttc ttttgcaatt agtaaatggg
     3121 tttctccaaa aagaactcgt tcttatcctt atgaaagagt atacaatact ctaagttcaa
     3181 cgaagaaaat tactattatc cccatcgtta aagatgaagg atttgaagga gaccgtgact
     3241 tcattcaatg ggacactgtc tcaatgatga gccttctcga tgtctatgta attcttgcct
     3301 attatgaaaa agcggaaaaa cataaaagcc gcaaagacaa aattacgaat caatctttca
     3361 gtaatgattt tatattatct aaaattaaag agataaaaaa ttatcacagt tccgctcttc
     3421 actggaatat aaacgagtta aaaataaatt tatataattt gatggataag gtaaagcact
     3481 cttattcgca aatagaaacg aatcttaaaa caaagttaca ttctacaaaa ggactagatg
     3541 actttcaaaa acagattaaa aaagatttag atttcttcat ggatagttct agaaaaaaat
     3601 ctatggaagc tcaagctaga gagattgaaa caaaccagcc aaaagaattt ttaaaaactt
     3661 tcacaaaagc taaaatcaca atcactaatt atttaggtgg aaaatatttt cttactatcg
     3721 atgaaattga attagaagaa aaaatgctgt atttaattga gtccaagcat agtaaatctt
     3781 ccgttcttcc aagtctaagc gatattaaag atggattatt gaaattaatt ttacttagca
     3841 atcttaaaga tgtaactgtt gatggacgta aaatcaagtg taaacctatt ttgaatttaa
     3901 cttctttaaa attaaaaggt tccattcaat ctgattctac tgaattagat aaattcttca
     3961 aagcgaatgc tttcaatgag aaaaataagg aattgattaa gaaaattttc aaagaggcta
     4021 atgagaataa ttttctcgtt agaataaaga gggctaagta aatgttaata aaaagtcctt
     4081 taagataccc tggtggaaaa agtagagcga ttgaacttat ttcaacgctt gttcctgaat
     4141 tccaagaatt tcgagagcct tttgttggtg gaggttcagt ttttatttat ttaaaacaaa
     4201 aatatccgaa taaaaagttt tggattaatg atttatattt tcagctttat acattttggg
     4261 atatgtgcca gaaggatttg agtgccgtag tgaagcaaat atggagttgg cgcaatgaat
     4321 ttgaaaatgg taaagactta cataaattct taaacgaaaa tatttcaaaa tttaattctg
     4381 ttcaaattgc aagtgctttc tttatttata accgaattac tttttcggga acaagtgaat
     4441 caggcgggta ttctgaacaa gcctttcgtg gacgatttac ggaatcaagt attgtaagat
     4501 tacaaaatat gaatcgtatt ctggaaaatt tacgtattac caatttagat tttgaagaag
     4561 ttgtaaatga aaaaggaaaa aatgttttta tttttttaga cccgccttac tattctgcta
     4621 caaagtccgc cttgtatgga aaaaatggga acatgcataa aaaatttgat catgttaggt
     4681 ttgctgaaac aatgcgaaat tgtaaacata aatggcttat tacttatgac gattgtgaac
     4741 atatacgaga actttttaaa tttgccaatg tcatgtcttg gaatttaacg tatggaatgc
     4801 gaaatgtaac ggattcatca gatcagaacg gcaaagagtt gtttatctct aattatttgg
     4861 aagagcttcc ttctatcaag caattggatt tgttttcaac gaagcctgta ttagtttaag
     4921 aatttattta gtattggtaa atagagtaag tattacactc gacctcgtgt cgaggctggt
     4981 tttagatgta gtcgtcagtt agactttgta ggagttcacg aaaataaaac gtcagcctaa
     5041 caaagattag ccgctacgaa gccacgagaa gatgtggctt cttgctccga gccgctggtt
     5101 gtttaagtgg aatttattag tttatttgga aagctatcgc aagtccagtg ccttcactac
     5161 tgtcacgaaa cttgcagaaa agagcaagtt tgcgcgccat ccgttcagtc acaggacttg
     5221 ctagttatac ggttggctcg gagacatgac tttaaaattc aaaagatgct accgcacttt
     5281 cgaattttaa agccacgtcg gctactctta acgttaggtg acatgccagc gagaatttta
     5341 aaaaatattg cggtatt
//
LOCUS       DAOQOS010000229         5221 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_9713_length_5221_cov_5.986643, whole genome shotgun sequence.
ACCESSION   DAOQOS010000229 DAOQOS010000000
VERSION     DAOQOS010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5221)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5221)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5221
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9713_length_5221_cov_5.986643"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..381)
                     /locus_tag="PLF66_25615"
     CDS             complement(<1..381)
                     /locus_tag="PLF66_25615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67493.1"
                     /translation="MYKKQIFKLGIGFFFFCLPIFSETVLLRNGTILNGKLKSQSINE
                     IVIVINNVEQKISKDKVLRVLYRDLSKAEARKALVDEIKKLSPKDREKLLSELKDKSL
                     ASSFEKKPEENPKTTEEKMEPQQET"
     gene            complement(468..2504)
                     /locus_tag="PLF66_25620"
     CDS             complement(468..2504)
                     /locus_tag="PLF66_25620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012389467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP repeat protein"
                     /protein_id="HMZ67494.1"
                     /translation="MNLSKIGEWNYADGKIIYYSVNEPIQDNLPSLNFPLKPTKFSIS
                     EPLPTGLSFNSVNGAISGTPTTSQAEKEYRFLASFPPIPDSPNNFSTLRMIIGEYNTT
                     NGINIPIRMQFNFPREFTYSNYSIAPNLPTGYNFDQNTGLITAESRNTTLDMGVFKVK
                     AARNDGVTVVGSGRFNITEWIHEAYLKAPNAEAIDNFGTAVAISSDTIVVGAIRESSN
                     QATITNGTLTSSNNSLTDAGAAYIFKRNGTLWTNEAYLKAPNVGGSDQFGNSVSISGD
                     TIVVGAAQESSNQTTITNGTTASADNSATAAGAAYVFRRNGATWTSEAYLKAPNANAG
                     DQFGYSVAIDGDTIVVGARQEDSNQTTITNGTLTSSNILAGVSGAAYVFRRTGSTWSN
                     EAYLKAPNAEANDQFGESVSISGDTIVVGAINEASNQTTITNGSTASSNNSASSSGAA
                     YVFRRTGSTWSNEAYLKAPNAESSDQFGYSVSISGDTIVIGAINEDSNQTTITNGATA
                     SGDNTAADSGAVYVFRRTGTTWVNEAYLKAPNAEGAGGSGPDRFGDSVSIAGDTIAVG
                     ALNEDSLQATISNGLLVQTSDTANNVSVGAVYVFKRTGTTWVNEAYLKAPNPTGGAFG
                     NGDKFGDCVSISGNTIVVGALNEDSNQTMITNGTIVTGDDSANNSGAVYVFRRK"
     gene            complement(2692..4794)
                     /locus_tag="PLF66_25625"
     CDS             complement(2692..4794)
                     /locus_tag="PLF66_25625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012389467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP repeat protein"
                     /protein_id="HMZ67495.1"
                     /translation="MKSLVVIVFSSLFLQCEIKQNPLAPNGPFGLFTFYLNLSKIGAW
                     SYSDPNPVYSKDLPIDTNVPKTSPLAIVKSYSMKTNLPNGLSLNTTNGYITGTPLELG
                     SSEVDIFANNDFYKEQYSTKLKITVAGFSYLPTYSFVFGSQVSITPTFSENLNASDFQ
                     FSITPSLPTGVSINPNTGVVSGTCAEKRNGSYTVTATTQSNKKFIASFQLNIVAWENE
                     AYLKAPNAEAGDNFGFSVSIDGDTIVVGSSGEDSLQTTITNGTMVSSDNSALNAGSAY
                     VFRRIGTTWANEAYLKAPNAETNDLFGTVVSISGDTIVVSTISEDSNQTTITNGTTAS
                     SDNSADLSGAAYVFRRTGTTWTNDAYLKAPNAELGDNFGGSVSISGDTIVVGGIGEDS
                     NQTTITNGTTNALNSSSGFGAAYVFRRTGSTWANEAYLKAPNAGMDDFFGWSVSVNND
                     TIVVGARDEDSNQTTITNGSIASANNSSTNSGAVYVFRRTGTIWVNEAYLKAPNAEAN
                     DKFGSAVSISGNTTVVAATGEASNQKTITNGITASADNSATSSGAVYVFRRSGTNWTN
                     EAYLKAPNADANDNFGNSISISADTIVVAAKSEASNQNTITNGSTASADNSATFSGAV
                     YVFRRTGTTWANEAYLKAPNPDNGDNFGESVSISGETIVVGASREDSNQTTITNGTTA
                     NANNTASDSGAVYVFRRK"
     gene            complement(4886..>5221)
                     /locus_tag="PLF66_25630"
     CDS             complement(4886..>5221)
                     /locus_tag="PLF66_25630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015681647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP repeat protein"
                     /protein_id="HMZ67496.1"
                     /translation="TIVVGATGEDSNQTTITNGTLSINGTGASVSGAAYVFKRTGNIW
                     VNEAYLKAPNAGTSDRFGEGGISISEDTIVVGARDEDSNQTTITNGSVVIDNNGAWGS
                     GAAYVFRRK"
BASE COUNT         1501 a         1080 c          908 g         1732 t
ORIGIN      
        1 tgtttcttgc tgtggttcca ttttttcttc tgttgtttta ggattctctt ctggtttttt
       61 ttcaaaggag gaagctaaac ttttatcctt caactctgaa agtaattttt ctctatcctt
      121 tggacttagc ttttttattt catcgactaa cgcttttctc gcttctgctt tgcttaaatc
      181 tctatacaaa actcgaagca ctttgtcttt agaaattttt tgttcaacat tatttatcac
      241 aattacaatt tcgttaatag attgggattt taattttcca ttcaagatag ttccatttcg
      301 aagtaaaact gtttccgaaa aaattggcag acagaaaaaa aagaatccga ttcctaattt
      361 aaaaatttgt ttcttataca tttttgcttt ttactttcct tactttattt tacttcaaaa
      421 aaaatacgat agatactaat tctattttct aatcggttag acctaattta tttgcgccta
      481 aacacataca ctgctccaga attatttgca gaatcatcgc cagtgacaat agttccattt
      541 gttatcattg tttgattaga gtcttcattt aaagctccca ctacaattgt atttcctgag
      601 atggaaacac aatcaccaaa tttatcacca tttccgaatg cacctccagt gggattaggc
      661 gcttttagat aagcttcgtt tacccatgta gttcccgttc ttttaaaaac atagaccgct
      721 ccaacagata cattatttgc agtatccgat gtttgaacca atagtccatt ggaaatagtt
      781 gcctgtaaag aatcttcatt caaagcaccc acggcaattg tatctcctgc tatggataca
      841 gaatcaccaa acctatcagg acctgatcct cctgcacctt ctgcatttgg agcttttaga
      901 taggcttcat ttacccaagt ggttcctgtt ctacggaaaa catagactgc ccctgaatcc
      961 gctgctgtat tatctccact ggcagtggct ccattggtaa ttgtagtttg attggaatct
     1021 tcattgattg ctcctattac aatggtatca cccgaaatag aaactgagta tccaaactga
     1081 tcgctacttt ccgcgttagg agctttcaaa taagcttcat ttgaccaagt tgagcctgtt
     1141 ctgcgaaaca cataagctgc acctgaagaa ctagctgaat tgttactact agcagtgcta
     1201 ccattcgtga tagtagtttg attcgaagct tcgttaattg cgcctacgac aatcgtatca
     1261 cccgaaatag aaacagattc accgaattga tcatttgctt ctgcatttgg tgctttgaga
     1321 taggcttcat ttgaccaagt agagccggtt ctgcgaaaca cataagctgc acctgaaact
     1381 ccagccaaaa tattggaaga agtaagcgtt ccattcgtaa tggtagtttg attcgaatct
     1441 tcttgacgag ctcctactac aatcgtatct ccatcgattg ctactgaata accaaattga
     1501 tctccagcgt ttgcatttgg ggctttgaga taggcttcac tagtccaagt cgctccattt
     1561 cgtctaaata cataggcagc tccggcagca gttgctgaat tgtctgcact agcagtagtt
     1621 ccgttggtaa tagttgtttg atttgagctt tcttgtgctg cgccaactac aattgtatca
     1681 cctgaaatgg atactgaatt tccaaattgg tcacttccac caacattagg tgcctttaaa
     1741 taagcttcat ttgtccaaag cgttccattt ctcttaaata tataggcagc tcccgcatct
     1801 gttaaagaat tattagagct tgtcaaagtt ccattcgtaa tggtagcctg attggaactt
     1861 tcccttattg ctccaactac aatagtatca ctagaaatag caacagcagt tccaaagtta
     1921 tcaatcgctt ccgcattcgg tgcttttagg taggcttcat gaatccactc ggtgatatta
     1981 aatctaccac ttccgacgac agtaactcca tcatttctag ctgccttgac cttaaaaact
     2041 cccatgtcaa gagttgtatt tctagactcc gctgtaatca gtcctgtatt ttggtcaaag
     2101 ttatagccgg ttggtaaatt tggtgcaatt gagtaattag aataagtaaa ctctctcgga
     2161 aaattaaatt gcattcgtat cggaatatta atcccgttcg tcgtattata ctcaccaatg
     2221 atcattcgca aagttgaaaa attattggga ctgtctggga ttggcggaaa acttgccaaa
     2281 aatctatatt ctttttccgc ttgagaagta gttggcgttc ctgaaatcgc accattgaca
     2341 gaattaaagc tgagtccagt tggaagtggt tcagaaatac taaattttgt tggtttgagt
     2401 ggaaaattta agctaggtaa attatcctga attggttcat taactgaata ataaattatt
     2461 ttaccatcag cataattcca ctctccgatc ttagataaat tcaaaaataa agtaaaaatt
     2521 ccaaaaggcc cattgggcga gaggggattt ttactaattt cgcaattcaa tattagaata
     2581 gataaggcaa ttacaaatag cgatttcata aggctttgat taatatttaa aattaattta
     2641 ctctaatttt tttagtttta tattagacta cattaggtgt ctagttataa atcatttccg
     2701 ccggaatacg tatacggcac cagaatcgct cgctgtatta tttgcattcg cagtcgttcc
     2761 atttgtaatg gttgtttggt ttgaatcttc tcgagatgca ccaactacaa ttgtttcacc
     2821 actgatggaa acggattctc caaagttgtc accgttatca ggatttggtg ctttcaggta
     2881 ggcttcattt gcccaagtag ttcctgttct gcgaaacaca tagactgccc cgctaaaagt
     2941 tgctgaatta tctgcacttg ctgtgcttcc atttgtaata gtattttgat tacttgcttc
     3001 actctttgcc gcaactacaa tcgtatctgc tgaaatagaa attgagtttc caaagttatc
     3061 atttgcatca gcgttaggtg cttttaaata agcctcattt gtccaattgg ttcccgatct
     3121 gcgaaaaaca tagactgccc cgctagaagt tgctgaatta tctgcacttg cagttattcc
     3181 atttgtaatt gttttttgat tgcttgcctc accagttgcc gcaaccacag tcgtatttcc
     3241 agaaattgaa actgctgacc caaatttatc attagcttca gcattgggtg cttttaaata
     3301 agcttcattc acccaaatgg ttcctgttct gcgaaacaca tagacagcac ctgaatttgt
     3361 agaagagtta tttgcacttg ctatgcttcc attcgtaatt gtagtttggt ttgaatcttc
     3421 gtctctcgca ccaaccacaa ttgtgtcatt atttacggaa actgaccagc caaaaaaatc
     3481 atccattccg gcattgggtg cttttaaata agcttcgttg gcccaagtgc ttcctgttcg
     3541 tcgaaacacg taggcagctc caaacccact tgaactattt aaagcattag ttgttccatt
     3601 tgtaattgta gtttggtttg aatcctctcc aattccaccg actacaatag tatctccact
     3661 aatagaaaca gagcccccaa aattatctcc aagctcagca tttggtgctt tcaagtaagc
     3721 atcatttgtc caagtagttc ctgttcgtcg aaacacatat gcagcacctg acaggtctgc
     3781 cgaattatcg gaactagctg ttgttccatt tgtaattgta gtttggtttg aatcttctga
     3841 tatagtacta acaacaatag tatctccaga aattgaaact actgtaccaa ataaatcgtt
     3901 cgtttctgca ttcggtgctt ttaaataggc ttcgtttgcc caagtagttc cgattctacg
     3961 aaacacatat gctgatccag cattaagtgc tgaattatct gaactaacca tggttccatt
     4021 tgtgattgtg gtttggagag aatcctcgcc agaagatccc accacaatgg tatccccatc
     4081 gatagaaact gaaaagccaa aattatcacc cgcttccgcg ttaggcgcct tcaaatacgc
     4141 ttcgttttcc caagctacga tattcaactg aaaacttgca ataaattttt tgttcgattg
     4201 agtagtcgcg gtcactgtat aagaaccatt tctcttttct gcacatgttc cagatacaac
     4261 acctgtgttt ggattgatac taacccctgt aggaagactc ggcgtaattg aaaactgaaa
     4321 gtcacttgcg ttcaaatttt cactaaatgt gggagttata ctgacttggc ttccaaagac
     4381 aaaagaatac gtgggtaaat aagaaaaacc tgccaccgta atcttaagct tagtagaata
     4441 ttgctcttta tagaaatcat tattagcaaa aatatctacc tcagaacttc ccaattctaa
     4501 aggagttcca gtaatataac cattggttgt atttaagcta agcccatttg gcagattcgt
     4561 tttcatcgaa taacttttta cgatagcaag aggagaggtt ttgggaacat tcgtatcaat
     4621 gggaagatct ttcgaataaa caggatttgg atcagaataa ctccaagctc cgattttaga
     4681 tagattcaaa tagaaagtaa aaagcccaaa cggtccattt ggcgcaagtg gattttgttt
     4741 gatttcacac tgcaaaaaca gggaagaaaa gacaattaca actagcgatt tcataaggtt
     4801 acaagcagta tttaaaataa atatcttgta aagtattttt tgttttaggt tttagttaga
     4861 ccaggatagg taactagttg taaatttatt tccttcggaa cacataagca gcacccgaac
     4921 cccaagcgcc attattgtca atcacaaccg aaccgtttgt gattgtggtt tggttagaat
     4981 cttcgtctct tgctccaacc acaattgtat cttctgaaat tgaaattcca ccttcaccaa
     5041 atctgtcaga agtgccagcg ttaggcgctt tgagatatgc ttcatttacc caaatattac
     5101 cagttctttt aaatacatag gctgcacctg aaaccgatgc tccagttcca tttatactta
     5161 aagttccatt tgtaattgtt gtttggttgg aatcttcccc tgttgcacca actacaattg
     5221 t
//
LOCUS       DAOQOS010000230         5108 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_10075_length_5108_cov_4.443499, whole genome shotgun sequence.
ACCESSION   DAOQOS010000230 DAOQOS010000000
VERSION     DAOQOS010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5108)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5108)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5108
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10075_length_5108_cov_4.443499"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(54..542)
                     /locus_tag="PLF66_25635"
     CDS             complement(54..542)
                     /locus_tag="PLF66_25635"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HMZ67497.1"
                     /translation="MKKGIRGFSLKLKLTVFLFAAILFITSFCFHFYRHITILPEDRL
                     IFLLLLFGCFFTFAALFRTILKNREMRLARESSLKYELLFENTGAAIGIFDTNGKILA
                     LNKQNAKTLGGVPSDFIGKTLYDLFPESADFHLNRFKKIIEEKKGQSFEDEFPLPGGS
                     KW"
     gene            1305..2714
                     /locus_tag="PLF66_25640"
     CDS             1305..2714
                     /locus_tag="PLF66_25640"
                     /inference="COORDINATES: protein motif:HMM:NF021778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HMZ67498.1"
                     /translation="MSSTTFQTGFLLRALNGDTFSYCGIGSESFRSGSSSSNLPTVTY
                     PTSSFTFTKNISIDTITPIITGSITSCTASPALVAGLTISATDCSISGRPLASQITTS
                     HTISFSGATGSSSTSINIEVRLQASKFIIVANSGSNNLNVFSINANGSLTSVPGSPFV
                     AGGAGARFVVVNPLGTYAYVANGGSASVSVFSINSTTGFLTPIAGSPFATAPVSPANP
                     SSLAMDPNGKFLYISPTPGNTLYVHSINQTTGALTYVNTYTTVNTANLSFITIDVTGR
                     FLYNTITNATNNIDGFRIDATTGALTQISGLPLTAGNNMGIGAVDPFTRFYYSISYSS
                     NTLSGYLVNSTTGALSPIAGSPFSDPAFGTSSTMAIDPTGKFVYVANNTTPTISGFSI
                     NQSTGAVTSIGSAVATGAAPGGIAVDPTGKFLYVTNGGPATVSGYSINATTGVLTAVP
                     GSPFSTVTTNPNAFSFVSY"
     gene            2790..3575
                     /locus_tag="PLF66_25645"
     CDS             2790..3575
                     /locus_tag="PLF66_25645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67499.1"
                     /translation="MSQMKNGKILIIVFLFLNCYVSEPRAKCEEVLRAFNFQAVYSFG
                     KACLYPTYDNDPENRNRCAVVALWNELCKKEPEKVYKMDLDIDLDGSEKKSREKEEGR
                     TFNTLFFLYAQNLIESNFVYEKGENLTDLLKYLVNQNYSQNVPDGETRSLTCSKGGIA
                     RTTFTQKDFVVNFQDCQHTRSYAANSSTLTWNGNLQGSITYNRVSNTYFLDTASLQGN
                     NVSYRGSISISGLGIRGIDEICRVSLSYSNYRGFYNFCNRSSQ"
     gene            complement(3849..4778)
                     /locus_tag="PLF66_25650"
     CDS             complement(3849..4778)
                     /locus_tag="PLF66_25650"
                     /inference="COORDINATES: protein motif:HMM:NF017206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HMZ67500.1"
                     /translation="MKDLHIIFGTGPLGRYTAEALLEMGKEVILINRSGKMDSIPNGA
                     KLIVGDILNLNNIKHIMIGATSIYQCAQPEYHEWIEKFPALQRAILELSIELKSRLIV
                     AENLYMYGKMGDKPFKETDPYRPISRKGQVRKEMTEELFDAHMQGKCKVASVRGSDFF
                     GPYEPINGKLIFQAALQRKKVNLLGNVNVPHSFTYVKDFGKALALCGTDERALGKTWH
                     VPSGKAYTQKEIVDLISQELGYKVKYQAAGKLILSIIGLFDKGAKEVIEMLYEFNSPF
                     ILDGSAMETTFGFKSTPMEIRIRETLEWIQSSK"
BASE COUNT         1529 a          919 c          915 g         1745 t
ORIGIN      
        1 ttttagtctc cttttggaag cctaaaatcg tgtccgtttt ttatgtaggg gttctaccat
       61 ttgctacctc caggcagagg aaactcatct tcaaaggatt gccctttttt ctcttctatg
      121 atttttttaa aacgatttaa atgaaagtct gctgattctg gaaataaatc atacaatgtt
      181 tttccaatga aatcagaagg aactcctcct agagtttttg cattttgttt attgagtgcg
      241 agtattttgc cattcgtatc aaaaatacca attgcagctc ctgtgttttc gaataacaat
      301 tcgtatttca gagagctctc tcgcgctaaa cgcatttctc tattttttag aatggttcga
      361 aataatgcag cgaatgtaaa aaagcaaccg aataaaagaa gaagaaaaat aagcctatct
      421 tcggggagaa tcgttatatg ccgatagaag tgaaaacaaa aagaagtaat aaataaaatt
      481 gctgcaaata aaaagacggt tagttttaac ttcaatgaaa atcctcggat tcctttcttc
      541 atttgccatt ttcccgaact aaatatatcg aaaccattag cataaaattt aatatagatt
      601 caactattat tcttagacct aaatatttta ttttagtaat ttcgcttaac tttctttgca
      661 ttgaaattat aagcttcttg gcaaataggt gaagctattg atcagtaacc actctagaga
      721 aaagagagaa ttttattctt cttttcattc aaaacaattt gaatacagtg tatccaatga
      781 gagtgagttg atagattact ggtgtattta cgtaagtcaa aatcatccag aaaaattttg
      841 tccattcctt ctaaggcgtc tctttctcct aatgtagaag ttgagtaaaa gttttttagg
      901 tggaaagttt cctatcgctt caagcctacc gacttggaag tggggaaata aaacgaagca
      961 gtatacgata ttctgtaaaa caatgtagca tgatttcatt ggcttttatt gggaatgagt
     1021 tagttggaaa ggatttagtt agagagtcaa ataaaaacgc aagatagccg ctaacttagg
     1081 aaggtgtcta accttgcaaa tctagaaact aatataaaaa tctaacttct tgagtctttt
     1141 aaaaattatt tctattgcct tttatatata aattgagtta tatactctaa tatatgaaat
     1201 ctttttctaa aggaaaaaac atgcggtcta tttttttacg tttccaattt gtttttttat
     1261 tcttctcctt ttgtagtcca gccattttca aaagtccctg tgatttgagt tcaaccacgt
     1321 ttcaaactgg atttcttctg agagccctca atggagatac gttttcatat tgtggaatag
     1381 gatctgaaag ttttaggtca ggctctagct ccagtaattt gccaacggtt acctatccaa
     1441 ccagttcttt tacctttact aaaaatattt caatcgacac aataactcct atcataacgg
     1501 gaagtatcac aagctgcact gcttcgccag cattagtcgc tggattgaca atatccgcta
     1561 cagactgttc aatcagtggt agaccacttg cctcacagat aaccacaagt catacaattt
     1621 cttttagtgg agcaaccgga tcttcttcca catcaattaa tatcgaggtt agattacaag
     1681 catctaagtt tatcattgta gcaaattcag gaagcaataa cttaaatgtt ttctctatca
     1741 atgcaaatgg aagcctaacg tcagttcccg gttctccttt tgtggcaggg ggggcagggg
     1801 ctcgatttgt tgtagtaaat cctcttggca cctatgctta tgtggctaat ggtggttctg
     1861 cgtcagtttc tgttttttct attaatagca cgactggttt tctaacgcct attgctggtt
     1921 ctccttttgc cactgcacca gtgagtccag caaacccatc ttctttggcg atggatccaa
     1981 atggaaagtt tttgtatata agcccaacgc ccggaaacac tctatacgtt cactccatca
     2041 atcaaacgac aggtgccttg acatatgtaa atacatacac cactgttaat actgcgaatc
     2101 tctcttttat tacgatcgat gtcaccggac ggtttttgta caatacgatt acgaatgcaa
     2161 ctaacaatat agatggattt cgaattgatg caaccacagg tgctttaaca caaatctctg
     2221 gcttgccgct cacagcaggg aataatatgg gtattggtgc ggttgatcca tttactagat
     2281 tttactattc aatctcttat agctcaaata cactcagtgg ttatctagtg aattcgacta
     2341 caggagcgtt aagtcctata gctgggtctc cctttagcga tcctgccttt ggcacttcta
     2401 gcactatggc aattgatccg acgggtaaat ttgtatatgt agcaaataat acgactccaa
     2461 cgatttcagg attttcgatc aatcaatcta ctggtgctgt gacaagtatc ggttctgctg
     2521 ttgccactgg tgctgctcca ggaggaattg cagttgatcc aacgggtaaa tttctatatg
     2581 tcaccaatgg tggtccagct actgtttctg gatattctat aaatgcaact acgggagttt
     2641 tgactgcagt tcctggctct cccttttcaa ccgttacaac aaatcctaac gcattcagtt
     2701 tcgtttccta ttgaattttt tgagttagat attctatgaa ttcgaaccat taaaaaaaaa
     2761 tagtgtttta gaatgaaaga aaaataagaa tgtcccaaat gaaaaatgga aaaatcctta
     2821 taatcgtatt cttattttta aattgctatg tatcagagcc tcgtgcgaaa tgcgaagagg
     2881 ttttgagggc attcaatttt caagctgtct attcttttgg taaagcttgt ttgtatccta
     2941 cgtatgacaa tgatcctgaa aatagaaata ggtgtgcggt agtcgctttg tggaatgaac
     3001 tttgtaaaaa agagccagag aaagtttata aaatggatct ggatatagat ttggatggct
     3061 ctgaaaaaaa gagtagggag aaagaagaag gaagaacctt taatactttg ttttttctct
     3121 atgctcagaa tctaattgaa agtaattttg tttacgaaaa aggagagaat ctaactgatc
     3181 tgctaaaata tttagtaaat caaaactatt cccaaaatgt gcccgatgga gagactagaa
     3241 gtttgacttg tagtaaagga ggaattgccc gaactacttt tacccaaaaa gatttcgtgg
     3301 tgaattttca agactgtcag cataccagat cctatgccgc taattcctct actcttactt
     3361 ggaacgggaa cttacaagga agcataacgt ataatagagt atcgaatact tactttttag
     3421 atactgcctc tctacaagga aataacgttt cttatagggg tagtatttcg atttccggtt
     3481 tgggaattag gggtatagat gaaatctgta gggtttctct ttcctatagt aattatcgag
     3541 gattttacaa tttttgtaat cggtcttctc aataacctag atacccaaac atggagtaaa
     3601 ctggatccat tttaaaaaca cctagtttag ctatttctac tttgaatgaa ggggtttaca
     3661 atgtgctcgt ttgatggagg gtataacttt atgcatgtag tttccggtgg cgcaattgtt
     3721 tactatccga atacgggcgg agcatatgca ctttggttta gataattttt gtgtttatag
     3781 tctattaact ttgatttgcc gttgtttaac ggcaaaattt gtagaggaaa aatcagtttg
     3841 gattttaatt attttgaaga ttgaatccat tccaaagttt cacggattct tatttccata
     3901 ggagtcgatt tgaatccaaa cgttgtttcc atagctgagc catccaaaat aaaaggagaa
     3961 ttgaattcat ataacatttc aatcacttct tttgctcctt tatcaaacaa gccaattatg
     4021 ctgagtatca atttacctgc cgcttggtat ttaactttgt atccaagttc ttgcgaaata
     4081 agatccacaa tttctttttg ggtgtaagct ttgcccgaag gaacatgcca tgtctttccc
     4141 aatgctcttt catctgttcc acatagagct aacgcttttc caaaatcttt aacataggta
     4201 aagctatggg gaacatttac atttcctagc aagttaactt ttttcctttg cagagcagct
     4261 tgaaaaatta gttttccatt gatcggttca taaggtccga aaaaatcact ccctcgaaca
     4321 cttgcaactt tgcattttcc ctgcatatgt gcatcgaaca attcctctgt catttctttt
     4381 ctgacctgac ctttgcgtga gattggtcga taaggatcag tttctttgaa aggtttgtcg
     4441 cccatttttc catacatgta taaattctct gccacaatca gtcgggattt caattcaata
     4501 ctcaattcca aaatcgctct ttgaagtgct ggaaatttct ctatccattc atggtattca
     4561 ggttgcgcac attgataaat ggatgttgcg ccaatcatga tatgttttat gttgtttaag
     4621 tttaaaatat cacccacaat cagttttgct ccattgggta tagagtccat tttgccggat
     4681 cgattgataa ggatgacttc ttttcccatc tctagtaatg cctctgcggt atatctacct
     4741 aaaggacctg tgccaaatat gatatgtaaa tctttcatgt aaattcctat gttgtcatca
     4801 tatacattat tgtagtcaat gtcaactatt tctattgaaa ctctactcac tatttttttt
     4861 gaaaaaacct caatcttttc tggtttgggg taaaccttgc attgccttat tttttaagaa
     4921 aagagattgt atgtcctatc gcctgatgga taaggtctca tcagatctat aaaaatgtaa
     4981 atatagcgat agccgataga tatataaacg ttatttcttt tctaattgac aatactttga
     5041 tagatttttt agatgaatta cttttagata tttaggcaat gaggtagata atattatgaa
     5101 aatatttg
//
LOCUS       DAOQOS010000231         5103 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_10085_length_5103_cov_6.759509, whole genome shotgun sequence.
ACCESSION   DAOQOS010000231 DAOQOS010000000
VERSION     DAOQOS010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 5103)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5103)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5103
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10085_length_5103_cov_6.759509"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..600
                     /locus_tag="PLF66_25655"
     CDS             <1..600
                     /locus_tag="PLF66_25655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67501.1"
                     /translation="KISIVDIETVPSDQEDVSKPLNPTIDYKSKEYLDFDAIINEEIE
                     QEEAELYAQSFFEEPDHSINLFDEGDNHNIIPSGNSTIDLDIVDTKAKIDLTFPPPIQ
                     DLEDLLDEEMELEKEKGIGSSQLPVDPSQTFLVKRINDVIQKEVLNEDSKGKIPVSNL
                     TKVNTESLEAIPSNTNSGSKKVSNPKISSPKFNPITLDD"
     gene            649..1524
                     /locus_tag="PLF66_25660"
     CDS             649..1524
                     /locus_tag="PLF66_25660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67502.1"
                     /translation="MSPNVLIKLFTPPQIVINYVKESMAIAHKRFLLSASASLIFTVL
                     LTLTYLSMIFLVFAIAYERETQNMLLGYLSLPIVSYFVCGFIRFYLRLVRGLPVNLKI
                     LFAGHRGYLHIMILLAAYYSLYILLFKIAFYMKDYDRVMQIRILLGVAAFFWTVARLI
                     FAPFFVIDADYTARQAMKASFLLTSGKTFKTFSLLIISVIFLVGGLIVNIALSFLIHE
                     VVFIFQSYSIYQVGIPIIILGSAYPFSLIIIAFVVSYDINLKNKYSRRKQLITQAAII
                     VKRKLEDTNIFEAVK"
     gene            1739..3631
                     /locus_tag="PLF66_25665"
     CDS             1739..3631
                     /locus_tag="PLF66_25665"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HMZ67503.1"
                     /translation="MAVLKKMKSILTHFLVLIFFINTQIGSQDLKILPIDLSSLKWYV
                     KKGFSTNDITTINSEQYSELNKFPIILNSLFKIPISFQELNEFTLFCSFQVNLDRLDK
                     GKEVAILFSGIGETWEVYLNGEKIKDEFQIDQNNIKKYRTMRNAVVLIPYGLLREENR
                     LVVHLAGFAPTSFLSPNILLGMRFKEGYLVDYRKNIEESIEQTSLLLFNAVYIFFGLY
                     HLFFFIRWTEKKYNLYFGIFSISISTYFLAFSNIAFDYVSDTRILFYLAYVSQPIALL
                     SFILFLFDYFNPSKKFSLFIKYTIVSNVFIILAFSILRINFYLSFLYLWYVLIVSQIF
                     YIFYFIVNATRKGFKDAIPMASSILIILLVVIWEILDTIVFQTGIKLLQFAYFGFIIS
                     MVFILANRFIEINWETRRLNIELTEQRDSFSKFVPIQFLDLLGKKSATDISLGECKLQ
                     EMTILFADIRDFTSLSESMSPEENFRFINSYLKRMSPVIERNNGFIDKFIGDAIMAIF
                     HFPEEALNAAISMHSELNTLNKYRASSGYRPLQIGIGLNTGMLMMGTIGHEDRLNTTV
                     IGDTVNLAARLETLTKEYKTPILISEFSVSKLSNLQEFNLREVDSVVVKGKTNSVRIF
                     ECKTDI"
     gene            3787..4617
                     /locus_tag="PLF66_25670"
     CDS             3787..4617
                     /locus_tag="PLF66_25670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016545730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-6-naphthoate synthase"
                     /protein_id="HMZ67504.1"
                     /translation="MKEQLTLAYSPCPNDTFLFYHLIHEDLSNKFSVKEELHDVEKLN
                     EYSEKNTFDVTKLSFFAFFHVISGYTLLNSGSALGRGCGPLLVKKKGKDLNKIKGEKI
                     LVPGIKTTANLLTNIYLNRDFEPYPIRYDWIMNKILEEEFNLGVIIHEERFTYEERGL
                     EKIVDLGDYWESLTGKPIPLGAIAIKRSIPIDLKREFDQSLRKSLELAYQFPEKTKKY
                     ILTNSQVKDEKVVQSHIDLYVNEFTKDIGDEGKDAVYALYEKAKTLGFLKDQKLDMFI
                     "
     gene            4725..5087
                     /locus_tag="PLF66_25675"
     CDS             4725..5087
                     /locus_tag="PLF66_25675"
                     /inference="COORDINATES: protein motif:HMM:NF019235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1564 family protein"
                     /protein_id="HMZ67505.1"
                     /translation="MNQDSITLSTQKISFPRKDFIYSEFSSTLLLPDRYSEIFYAKCK
                     YHGGVREYISYLLSRYQLFIANGLVPGYSNVTTKYQEKGQNLQRIAFRPKPEDWAELK
                     LYRISFGIEPLHKKRTRF"
BASE COUNT         1738 a          736 c          815 g         1814 t
ORIGIN      
        1 aaaatatcaa tagtagatat tgaaaccgtt ccttctgatc aagaagatgt atcgaaacct
       61 ttgaatccaa cgattgatta taagtcaaaa gaatatctag attttgatgc aattataaat
      121 gaagagatag agcaagaaga agcagagttg tatgctcaat ctttttttga agagccagat
      181 cattccataa atctttttga cgaaggggat aatcataata taattccttc agggaattcc
      241 actattgatt tagatattgt ggatacaaaa gcgaagatag atttaacgtt ccctccgcct
      301 attcaagatc ttgaagattt attagatgaa gaaatggagt tagaaaagga aaaaggaatt
      361 gggagtagcc aattgcctgt ggatcctagt cagacatttc tagtgaaacg tattaatgat
      421 gtaatacaaa aagaagtttt gaatgaagat tcaaaaggga agattcccgt ttcaaattta
      481 actaaggtga atactgaatc gttagaagct attccttcga atacaaattc tggatctaaa
      541 aaggtttcta atccaaaaat atcaagtccc aagtttaatc caattacact ggatgattaa
      601 aatttttttc ttgcttttca atgccactca ataagacgct aaagatacat gagtccaaat
      661 gtattaatta aactatttac tcctccgcaa attgttatta attatgtaaa ggaaagtatg
      721 gcaattgctc ataaaaggtt tttgttatca gcatctgcta gtcttatttt tacagtactt
      781 ttgactttga cttatcttag tatgattttc ttggtctttg ctattgcata tgaacgagaa
      841 acccagaata tgctactagg atatttatcc ctgccgatag tatcatactt tgtgtgtggg
      901 tttattcgct tttatttacg attagtaaga ggtcttcctg taaatttaaa aatactattt
      961 gcaggtcata gagggtattt gcatataatg attctattag cagcctacta ctctctttat
     1021 atattgctat ttaagattgc attttatatg aaagactatg acagagttat gcaaattcgg
     1081 attttgttag gcgttgctgc ttttttttgg actgtagctc gtttgatctt tgctcccttt
     1141 tttgttattg atgcagatta tacggcgagg caagccatga aggcatcttt tcttttaaca
     1201 agtggcaaaa ccttcaagac ttttagttta ctgattattt ctgtaatatt tttggttggt
     1261 ggtttgattg tcaatatcgc actttctttt ctcatccacg aggtcgtatt tatttttcag
     1321 tcttattcta tttatcaagt aggcatacca atcataattc tgggttctgc ctatccattt
     1381 tccttgatca tcattgcttt cgttgttagt tatgatatta atctgaaaaa taagtattct
     1441 agacgaaaac agcttataac acaagcagct attattgtga aaagaaaatt agaagatact
     1501 aatatatttg aagctgttaa ataaaaccta tcgtttaata attatattta tcgaattctt
     1561 ataggtaatt atgtgttgaa tttattttgt tttagaaaat tttttactct acaaaaatcc
     1621 atcttttaat aaattattgt ctctgttaaa cttcattttt gcgtaaaagc aaacaattct
     1681 ttttcaagaa tattttttgt gaagaaatga tgtatataaa tctaaaggaa tactctttat
     1741 ggcagttcta aaaaagatga aaagtatttt gacacatttt ttggtcttaa ttttttttat
     1801 aaatacacag attggatcac aagatctaaa aatactgcct atcgatttat cctccctaaa
     1861 atggtatgtt aaaaaaggtt tttcgactaa cgacattacg actataaatt ccgaacaata
     1921 tagtgaatta aataaatttc caattatttt aaattctcta tttaaaattc ctatctcttt
     1981 tcaagagtta aatgaattta ctcttttttg ttcatttcaa gtgaatctag atcgtttaga
     2041 taaaggaaaa gaagtcgcta ttttattttc agggattgga gaaacatggg aagtatattt
     2101 aaatggggaa aaaattaagg atgagtttca aattgatcag aataatataa aaaaatatag
     2161 aaccatgaga aatgcagtag ttctaattcc ttatggatta ttgcgggaag aaaaccgctt
     2221 ggttgtgcat ttagctggtt ttgctcctac ttccttttta tctccgaata ttttattggg
     2281 catgcgattt aaagaaggtt atctggttga ttatagaaag aatattgaag aaagtatcga
     2341 acaaacatcc ctgttacttt ttaatgcagt ttatattttc tttggactgt atcatttgtt
     2401 cttttttata agatggactg aaaagaaata taatctatat tttggcattt tttccatatc
     2461 tatatcaacc tattttttag ctttttcaaa tattgccttt gattatgtat cagatactag
     2521 aattttattt tatctagctt atgtatcgca acccattgcc ctactatctt ttatattatt
     2581 cttgtttgat tatttcaatc catctaagaa attctctctt tttattaaat atacaatagt
     2641 atctaacgtt tttataatat tggctttctc aattctgaga attaattttt atcttagttt
     2701 tttatactta tggtatgtat taattgtttc tcagatattt tatatctttt actttattgt
     2761 caatgcaact cgtaaaggtt ttaaagatgc aattcctatg gcatctagta tcttaattat
     2821 attactggtt gttatatggg aaattctgga tactatcgta tttcagactg gtattaaatt
     2881 gctgcaattt gcatacttcg gttttattat ctctatggta tttatacttg caaatcgttt
     2941 catcgaaatc aattgggaaa cgagaagatt aaatattgag ttaacagaac agagagattc
     3001 cttttctaaa tttgttccaa ttcaattctt agatttacta ggtaaaaaat ctgcaactga
     3061 tatatctctg ggtgaatgca aattacaaga gatgacgatt ctctttgcag atattcgtga
     3121 tttcacttcc ctctccgagt ctatgtcacc agaagaaaat tttcgattca taaattccta
     3181 cttaaaaaga atgagtccgg taattgagag aaacaatgga tttatagata aatttattgg
     3241 agatgcaatc atggcaatct ttcattttcc tgaagaggca ttaaacgctg caatatctat
     3301 gcatagcgaa ttgaatacat taaacaaata tagagcatct tccggctatc gtcctttaca
     3361 aataggaatc ggattaaata caggtatgct tatgatggga actataggac atgaggatag
     3421 acttaataca acagtaattg gtgataccgt taatctagct gctcgattag aaactttaac
     3481 caaggaatat aagacaccta ttttgatttc tgagttttct gtttcaaaac tttcaaattt
     3541 acaagagttt aatttacgtg aagttgattc tgtagttgta aaagggaaaa cgaactcagt
     3601 aagaatcttt gaatgtaaaa ctgatattta gcttttatag agaataaaaa ctcagttgaa
     3661 acgaaaggat tacagagtgc cgacaggcac cctggctcaa ctgtatttag tgtcatagag
     3721 aatattagat ggacagtaaa atctttttca tttacaaatt taaaacaaat gataatatga
     3781 ttagatatga aagagcaact cacattagcc tattcaccct gtcctaatga tacattttta
     3841 ttttatcatc tgattcatga ggatttatca aataaatttt ctgttaaaga agaattgcat
     3901 gatgttgaaa aattaaatga atattctgaa aaaaatacat tcgatgtaac caaactttct
     3961 ttcttcgctt tttttcatgt aataagcggt tacacgttgt taaactctgg ttctgctttg
     4021 ggtagaggtt gtggtccatt gttagtaaag aaaaagggaa aagatttaaa taaaatcaag
     4081 ggagaaaaaa tattagttcc tggaatcaaa acgacagcaa atctacttac caatatttat
     4141 ctaaatagag attttgaacc ctaccctatt cgatatgatt ggataatgaa taaaatttta
     4201 gaagaagagt ttaatttagg cgtaatcatt catgaagaga gatttaccta cgaagaaaga
     4261 ggattagaaa aaattgtaga tcttggagat tattgggaga gtttaacagg caaacctatc
     4321 ccacttggag caattgcaat taaacgctct atcccaatag atctgaaaag agaatttgat
     4381 caatctctcc gaaaaagttt agaacttgcc tatcaatttc ctgaaaaaac taaaaaatat
     4441 attttgacaa actcacaagt gaaagatgaa aaagttgtac aaagccatat agatctttat
     4501 gtaaatgagt tcactaaaga tattggagat gaaggtaaag atgcagtgta tgcgttatac
     4561 gagaaagcta agacccttgg atttctaaaa gatcaaaaat tggatatgtt tatctaatta
     4621 cataaccttt aaactaggaa aaaataaata caagtcttaa aattaattca aaaatcgcgc
     4681 tcaaattcaa agatttaacc tctattatta atagaggtaa atctatgaat caagactcca
     4741 taactctttc gacacagaaa atctctttcc caagaaaaga ttttatttat tctgaattta
     4801 gttcgactct tttacttcca gatcgatatt ctgaaatatt ctatgctaaa tgcaaatatc
     4861 atggcggagt tcgagagtat atcagctatc tactttctag ataccaactt tttattgcaa
     4921 atggacttgt tcctggttat tcaaatgtta ctactaagta tcaagaaaag ggccaaaatc
     4981 ttcaaaggat agcttttcgc cctaaaccag aagactgggc agaactgaaa ctttatcgta
     5041 ttagttttgg tattgaaccc ctacataaaa aaaggacacg attttaggct tccaaaagga
     5101 gac
//
LOCUS       DAOQOS010000232         4865 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_10841_length_4865_cov_5.158628, whole genome shotgun sequence.
ACCESSION   DAOQOS010000232 DAOQOS010000000
VERSION     DAOQOS010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4865)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4865)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4865
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10841_length_4865_cov_5.158628"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            741..3494
                     /locus_tag="PLF66_25680"
     CDS             741..3494
                     /locus_tag="PLF66_25680"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF012532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HMZ67506.1"
                     /translation="MKENWNEVKESFQKIDKDRAFLYGCFYEVLLELGEETIAEILKS
                     IEEEKNIPEIQEEDLNEKLIQAFSIYFQIVSMVEENAANQHRRKLESSQGEARIRGSY
                     GETFQLFKEKKISENQILESIRSIQATPVLTAHPTEAKRATVLSIHRDIFLLLVKREN
                     PIWTPEESESIRQEIKSHLERLWRTGEVYLEKPDIPSERRNVLHYFENVFPEALTLLD
                     RRFSYLWKKAGYSERYLENYKNYPVIRFGSWVGGDRDGHPFVTSDVTEESLLELRSHA
                     LSLIQKKVNQLAASLSFSDRLYFVPSWLIEKIHSDKKKFGEEGERCVTRNPNEPYRQF
                     LNLILFRLNQTQKDHPLGYKKASELLEDLDLLGKALIENKAKRIMREEVLPLTRIVQC
                     FGFHLARLDIRQNSKFHETALTELLEISGIENFHYSEWSEDKRRDFLNQELKSPRPFT
                     IGDLQSDKEAGAVLSCYTVLKNTINKYGSDGIGSLIVSMTRDLSDLLVVYIFAREAGL
                     MKHSQKGLACQLPLVPLFETIDDLDRSPEILDLFLSHPITQNSLELQRERNNGELIQE
                     VMLGYSDSNKDGGIFASRWNLYKAEKKLTEIAQKYGVKLNFFHGRGGTISRGGGKIHR
                     FIESMPIKTLTGNLKMTVQGETIGQQYANLMNAVYNLELLSSGILRETVFNHSIQRED
                     KGIDSILNFLSEESCKTYEKLIKAPEFIPFYSEATPIDAIENSKIGSRPARRTGKRTL
                     GDLRAIPWVFSWSQSRFNITGWFGVGSALEKLKESRPNDFSLLKEKVNDWSFLKYNMI
                     HIETNLLNSDEVIMREYASLVKQDSVRNTIFQMIQTEYQKTYRMIEEVMGANLKERRV
                     SHLHSLNLRTKWLKFLHQTQIRILKIWRNKLENGSKEEADKILPKLLLTINGIAGGLK
                     NTG"
     gene            3906..>4865
                     /locus_tag="PLF66_25685"
     CDS             3906..>4865
                     /locus_tag="PLF66_25685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HMZ67507.1"
                     /translation="MLSKLQEGNIKTNKVEKVVDKPKGFTIQMKLMGIISLLFVLTVS
                     IIILIATKFFRDETEARVDDMNLQNVRITGSKVKTDIAAIIDKATQMIVILQSDSRPE
                     KYIQLFFKNDPDFLMLGLYRKENEKLTPVKLIINQDYLNDYQLKKEDLESIINLNQTI
                     FERSFEGEPILYNASPGAKEPVFAISFPQTDGDKQDIIISLIKLDKIESAFKKSGITE
                     IFLVNGDGLVIAHDDPKVLFTSANYSDMPIVDEMLKSTIANGKKTYKDSTGKEFIGAY
                     QKIGFANSGVISVVEEERAMESVYKIQSWNLKIMIIGVSAALII"
BASE COUNT         1785 a          834 c          843 g         1403 t
ORIGIN      
        1 gtgttcatcc gtgtctatcg gtggtaatct tttaatgtat tcttcaaacg acaattctcc
       61 ttattcttgg gattcaattt ccgaatccat tagcaaacac aaaagcattg catggagaga
      121 ggaaggcttg cgagagaaaa agcacacacg ccaatgagac aaatcgcttg gtatagtggt
      181 tcgtatagtg ttgttatagt ggttgctata ctcaaaaaag tttttcaata taggatacag
      241 tataggaaaa gaattccaaa tcgatccttt cacgttagtg ttcattttaa aaatgtttag
      301 tatttgctca gaaggaacga tccagaattt tcgaatagtt caacggaaac aaaaagacaa
      361 gtagaacgag aagctgcctt ctacaatatc gaaatgattt ttttgcagga tatagaatta
      421 aatcagcgat tgctactgag ttttattagt ggacagcgga gaaactcaaa gagtctatca
      481 tcaaaaaaga ctaaaagaag aagcaagcca agcttcatcc ctcgattctg tattggagga
      541 aattcccagt ttagtaaaat caaagaacaa gaaaacaaac gatgccaaaa acaaaataat
      601 agagtgaaaa ccaaaacaac tctctcttta gagaatcgat tttttagtaa aatgttttct
      661 attcctttta ttcataaccc acttctaatg tctaaagaat aatattatca ataccattca
      721 aagataaaga ggagaatcgt gtgaaagaga attggaatga agtgaaagaa agttttcaaa
      781 aaattgacaa ggatagagct tttttatacg gatgttttta tgaagttctt ttggaacttg
      841 gagaagaaac cattgcagaa attttaaaat ctattgaaga agaaaaaaac attcccgaaa
      901 tacaagaaga agatttaaac gaaaaattaa tccaagcttt ttctatctac tttcaaatcg
      961 ttagtatggt tgaggaaaat gccgccaacc aacatagaag aaaattagaa tcctctcaag
     1021 gagaagcaag aatccgtgga agctatggcg aaacttttca acttttcaaa gagaaaaaaa
     1081 tttctgaaaa tcaaatctta gaaagcattc gatccattca ggcaactcct gttctcacag
     1141 cccatccaac cgaagccaag agagccactg ttctctccat tcaccgcgat atttttcttt
     1201 tactcgtaaa acgagaaaat ccaatttgga ctcccgaaga atccgaaagt attcgccagg
     1261 aaattaaatc tcatctagaa agattgtgga gaaccggtga agtctattta gaaaaaccag
     1321 atatcccttc tgaacgaaga aatgttctac actatttcga aaatgttttt cccgaagcac
     1381 tcacgttact cgatagacgt ttttcctatc tttggaaaaa agcaggttat tcagagcgtt
     1441 atctggaaaa ttataaaaat tatccagtca ttcgttttgg gtcttgggtt ggaggagata
     1501 gagacggtca tccgtttgtt acttccgatg ttaccgaaga aagtctttta gaacttagat
     1561 ctcatgctct ttctctcatt caaaaaaaag taaatcaatt agcggcttct cttagttttt
     1621 ccgatcggct ctactttgtt ccaagttggt tgatcgaaaa aattcactca gataagaaaa
     1681 agttcggaga agagggagaa agatgcgtta ctcgaaatcc aaatgagccg tatcgtcaat
     1741 ttcttaattt aattctcttt agattaaacc aaactcaaaa agatcatccc ttgggataca
     1801 aaaaagcctc tgaattatta gaagatttag acttacttgg aaaagcctta atcgaaaaca
     1861 aagccaaaag gattatgcga gaagaagttt taccattaac tagaattgtt caatgttttg
     1921 gattccatct agctcgctta gatattagac aaaatagtaa atttcatgaa actgctttaa
     1981 cagagctttt agaaatttct ggcattgaaa actttcatta ttcagaatgg agtgaagaca
     2041 aacggagaga ttttttaaat caagaactga aaagcccaag accttttacg ataggcgatt
     2101 tacaatcaga taaagaagcc ggagcggttc tttcttgtta taccgtttta aaaaatacaa
     2161 ttaacaaata tggatcagat ggaatcggtt ctttaattgt aagtatgaca agagatttat
     2221 ctgatttgct cgttgtctat atttttgcaa gagaagctgg tttaatgaag cattctcaaa
     2281 aagggcttgc ctgccagctt cctcttgtcc ctctcttcga aacgatagat gacttagatc
     2341 gaagtcctga aatcttagat ttatttttaa gtcatcctat cactcaaaac tctcttgagc
     2401 tacaaagaga aagaaacaac ggagaattaa tccaggaagt aatgttagga tacagtgata
     2461 gcaacaaaga tggaggaatc tttgctagta gatggaatct atacaaagca gagaaaaaac
     2521 tcacagagat tgcacaaaag tatggagtca aattaaactt ctttcatgga cgcggtggca
     2581 ccatcagtcg tggcggcggt aaaattcacc gatttataga atccatgcca attaagactc
     2641 ttacaggaaa tttaaaaatg actgttcaag gagaaactat cggtcaacaa tacgccaatt
     2701 taatgaatgc agtttacaat cttgaattac tctcatcagg aattctaaga gaaactgttt
     2761 tcaatcattc cattcaaaga gaagacaaag gaattgactc aatccttaat ttcctttcag
     2821 aagaaagttg caagacatat gaaaaactca tcaaagctcc tgaatttatt ccattctaca
     2881 gcgaagctac accgatagac gctatagaaa atagtaaaat aggttcaaga cctgctcgaa
     2941 gaacaggcaa aagaacgtta ggagatttaa gagcaattcc atgggtcttt agttggagtc
     3001 aatctaggtt taatataact ggttggtttg gagtaggttc tgccttagaa aaattaaaag
     3061 aatctagacc aaatgatttt agcttattaa aagaaaaggt aaatgattgg tcttttctaa
     3121 aatacaatat gattcacata gaaacaaatc tattaaattc agatgaggta attatgcgtg
     3181 aatatgcttc cctcgtaaaa caagacagcg ttcgaaatac aatttttcaa atgattcaaa
     3241 ccgaatatca aaaaacctat agaatgattg aagaagtaat gggtgccaat ctaaaagaaa
     3301 gacgtgtgag tcatctgcac tctttaaacc ttagaacaaa atggcttaaa tttttacatc
     3361 aaacccaaat tcgaattcta aagatttgga gaaataaatt ggaaaatgga agcaaagaag
     3421 aagcagataa aattctccct aaattgcttc tcacgatcaa tggaatcgca ggtggtctta
     3481 aaaatacggg ctaattgagt ctataacgca tagatacaaa agacaatctg cacaagaact
     3541 ggtcttttgt atctttctaa aaaaaccggt tattactagt aaatatacgt gaaaaaggct
     3601 tattcttttt tcaaaaaaaa ggattctccc aatcgatttc aaaactcttt tgaaataaaa
     3661 aactgcctca aaataggaaa ccagaatttg catatacatt ctgcaaaaaa atcaatattt
     3721 ccaaaaagac ttaggtttta aagagaatct agagcagcaa ttgagcaatt tctacttgct
     3781 aataattttt aaatacatag tcttcccata aaaggctctt catttcaaga ttgaaatgtc
     3841 aatttttttc ttgaaattat agattgctac atttactatg caaacttcat aaaaggagtt
     3901 atactttgct ttctaaacta caggagggaa acataaaaac taacaaagtt gaaaaagtag
     3961 tagataaacc aaaaggcttt accattcaaa tgaagctaat gggcattatc tcccttctct
     4021 ttgtcctcac tgtatcgatt atcatcttaa tcgctacaaa atttttccgc gatgaaacag
     4081 aagcgcgtgt agatgatatg aacctacaaa atgttcgtat cacaggttcc aaggttaaga
     4141 ctgatattgc cgctattatc gataaagcaa cgcaaatgat tgtaatcctg caatccgact
     4201 ctcgtcccga aaaatacatc caactctttt ttaaaaatga tcctgacttt ctaatgctcg
     4261 gtctctaccg aaaagaaaat gaaaagctaa cacctgttaa actcataatc aatcaagact
     4321 atttaaatga ctatcaatta aaaaaagaag atttagaatc gattataaat ctcaatcaaa
     4381 ctatttttga acgctccttt gaaggagaac caattctcta caacgcaagt cccggagcca
     4441 aagagccagt ctttgcaatt agttttccac aaaccgatgg agacaaacaa gacatcatca
     4501 tctctctaat caagttggat aaaattgagt ctgcttttaa aaaatctggt attacagaaa
     4561 tttttcttgt gaatggggat ggtttggtga ttgcacatga cgatccaaaa gttctattca
     4621 cctctgctaa ttatagcgat atgccaatcg tagatgaaat gttaaaaagc acaatagcaa
     4681 acgggaaaaa gacatataaa gactcaactg gaaaagaatt tattggagcc tatcaaaaaa
     4741 ttggtttcgc aaacagtgga gtcatttcag tcgtagaaga agaaagagct atggaatctg
     4801 tttataaaat ccaaagctgg aacttaaaga tcatgatcat tggcgttagt gctgccctta
     4861 tcatc
//
LOCUS       DAOQOS010000233         4793 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_11097_length_4793_cov_4.329675, whole genome shotgun sequence.
ACCESSION   DAOQOS010000233 DAOQOS010000000
VERSION     DAOQOS010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4793)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4793)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4793
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11097_length_4793_cov_4.329675"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            232..831
                     /locus_tag="PLF66_25690"
     CDS             232..831
                     /locus_tag="PLF66_25690"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HMZ67508.1"
                     /translation="MDPLELGKKYDKIAEWWNEKHFHSEYGIPQIRKAIEFVSEPGKV
                     LDIGCGAGGRFIRLFESRNYTITAIDVSSKMIDLAKLNHPNHRFINQDICQWETDEKF
                     NLIVAWDSIFHLPLSMQKPVLAKLCSLLEKNGVLVYTFGNAEGDHLYEWHGDHFYYSS
                     VGINENIQILMKNGLTILHLELDQYPEKHVYTIAKKISD"
     gene            complement(1300..1713)
                     /locus_tag="PLF66_25695"
     CDS             complement(1300..1713)
                     /locus_tag="PLF66_25695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017860520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67509.1"
                     /translation="MPIKPENKNLYPKNWKEISLAVRQRANGKCEFCGIENYSLGHWV
                     NDRFIKSEEAWNEMNSNGVDLYESIPTEKPFVKIVLTVAHLDHNPSNCDDSNLKALCQ
                     KCHLTYDAKHHAANARKTRNRKKYGVLSLFPISNY"
     gene            1974..2198
                     /locus_tag="PLF66_25700"
     CDS             1974..2198
                     /locus_tag="PLF66_25700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67510.1"
                     /translation="MNEKELRIIIARIRSEKEVLLTEFRELASQFSVREFSEKIEVDH
                     SRIHKILNGKLDVSIETLCEYIERFLKVKK"
     gene            2195..2551
                     /locus_tag="PLF66_25705"
     CDS             2195..2551
                     /locus_tag="PLF66_25705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67511.1"
                     /translation="MKTFLYIVIYLGVVIFHLMDAYLAAIAYGFPLGLYLFLFSLCIV
                     GVLLSLWRDSKLLLAACFLEFFLAGLLAIGNGRNLFDLDFSVLFLPSILRIIVLVLHY
                     FSIFELSKIFLHRERY"
     gene            complement(2553..2735)
                     /locus_tag="PLF66_25710"
     CDS             complement(2553..2735)
                     /locus_tag="PLF66_25710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67512.1"
                     /translation="MKQILSKFEHGNTKYGADDLEKFQALRDKGYTIKQIIQELNLPT
                     NARALGKFLNRNGIRK"
     gene            complement(2953..3381)
                     /locus_tag="PLF66_25715"
     CDS             complement(2953..3381)
                     /locus_tag="PLF66_25715"
                     /inference="COORDINATES: protein motif:HMM:NF018011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1018 domain-containing protein"
                     /protein_id="HMZ67513.1"
                     /translation="MEPKKLRTIFAYKKELQLTEEELYLHLNLICKKDSISKLSDLEA
                     DKFIRSLRSKLPKKPAPTRKYKTIRSKKNPNIVAIATREQREYLSNLLSQINSQGIYK
                     LTLEGMSQRQFKKSEKLLTRNDARILTEAAKNILRREVVG"
     gene            complement(3417..3599)
                     /locus_tag="PLF66_25720"
     CDS             complement(3417..3599)
                     /locus_tag="PLF66_25720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67514.1"
                     /translation="MKPVAQFQDDYDFIDKLTGLRIKILKEKSAWIGYQKAYRYIKRQ
                     NILTHNTQLTMHCELN"
     gene            complement(3612..3920)
                     /locus_tag="PLF66_25725"
     CDS             complement(3612..3920)
                     /locus_tag="PLF66_25725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67515.1"
                     /translation="MNQIKFSHEYFKLGNIEQDHPVQLVEVFQKPTEEFSDEFKEYDT
                     AYYDGKTIANYPLGKGLHLILLFRDSKGNLFTTVRRFTHEKFVYYNNNRGKQFSIVFT
                     "
     gene            complement(3917..4147)
                     /locus_tag="PLF66_25730"
     CDS             complement(3917..4147)
                     /locus_tag="PLF66_25730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67516.1"
                     /translation="MKCPHCESEFDATMKELIQLHNEDMWINHCEDFFRRGAQVSTID
                     RLPPELKNSERIKKYYVILNAKIKKREQEKAI"
     gene            complement(4148..4714)
                     /locus_tag="PLF66_25735"
     CDS             complement(4148..4714)
                     /locus_tag="PLF66_25735"
                     /inference="COORDINATES: protein motif:HMM:NF019000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0042262 - DNA protection [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HMZ67517.1"
                     /translation="MAKAKPQPTETIVIYEYEQSDLPKAIAEIARLTTEIKKAEDRTN
                     ESISILQTTLAEQIEPLKQEIKKISLSVKKFTDNNREKLFKGGEKTVKLETGDLSYRS
                     AGKHVSQNSSVKLITSILEQNNLIEAKEKFVKKMDKVFIRTKLELNKDAILQNPETAK
                     VVTGVDVEDGEESFYIKPYSTNTELEVA"
BASE COUNT         1337 a          777 c          885 g         1794 t
ORIGIN      
        1 aatctgaaaa attccttgtt ttgaaagaag tctaccactc tgttaggcat tttttttccc
       61 gacatgattt gaaattcttc tatgtaattt tgaatatcca tcgtttcact atttgcattt
      121 tgctttgcca agagaacccc ccattattga ttgtattgtc catgatacaa aactggaaat
      181 gattagacaa ttgaataatt tccaagtact aaataggagt gtatatcaaa tatggatcct
      241 ttggaattag gaaaaaagta tgataagata gcggagtggt ggaacgaaaa acattttcat
      301 tctgaatatg gaattccaca gattcgaaaa gccattgagt ttgtttcaga gccaggaaag
      361 gttttagata ttggatgtgg tgctgggggg agatttattc gcttatttga atctagaaat
      421 tatacgataa ccgcaataga tgtatcttct aaaatgatag acttagcaaa actcaatcat
      481 ccaaatcaca gatttataaa tcaagatata tgtcaatggg agactgatga aaaatttaat
      541 ttgattgttg cgtgggatag cattttccat ttaccattgt ctatgcaaaa gccagtcttg
      601 gctaaactct gcagtcttct tgaaaaaaat ggggttctgg tttatacatt tggaaatgca
      661 gagggtgatc atctctatga gtggcatggg gatcactttt attatagttc tgttggtata
      721 aatgagaaca ttcaaattct tatgaagaat ggattaacaa ttctgcattt agagttagat
      781 cagtatccag aaaagcatgt ttatacgatc gcaaagaaaa tttctgattg atcagaattt
      841 taaaaaatca acgaaaaaat tgaaataaag atcaatccgt ttcttagatt caaaaataaa
      901 tggatggatc tatccataca gtagatctga gtaatcgatt ttcaaaaata ctactctttc
      961 ctaagaacgg atactcttgg ttagcctcaa ctagaagttt gtagggatta aataaggaag
     1021 gatattagaa aatagctgtt agaaggctag tccatgatta aaagaaatct taaaaatatt
     1081 cttgcttcat tgattttcca aggagcttag cattttatat aagggataaa ccataatcaa
     1141 taatgtgcac ttaaatgtta actagaatta cagtttaata aagaataaca aggaaatgaa
     1201 gccattctaa gaagttttta ttttatcatt ttctcataaa ctgggaataa aaagtatata
     1261 gattgagtga tattttctga ataatcaacc tagaaattgc taataattgg agataggaaa
     1321 caaagataga acgccatact ttttcctatt acgagtttta cgggcgttag ctgcatggtg
     1381 tttagcatca taagttaagt ggcatttttg gcagagggct tttaaatttg aatcatcaca
     1441 attactcgga ttgtgatcga gatgagcgac agtcaggact atttttacaa aaggtttttc
     1501 tgttggaatt gattcgtaaa gatctactcc attagaattc atttcattcc aagcctcttc
     1561 tgattttata aatctatcgt ttacccagtg acctaaagaa taattttcaa taccgcaaaa
     1621 ttcgcattta ccgtttgctc tttggcggac cgctagagaa atttctttcc agtttttagg
     1681 gtatagattt ttattttcag gtttaatagg cattacttaa ttcttctttt tttattctta
     1741 ttgttgacaa aaaaaacaac atatagtaag ttgattgtaa ggaggttgac tatgataatt
     1801 atcttagtat tcaccttcgg attagctttc ctaattgctt ggaaagtaat cagcttcatc
     1861 ctagccgatg cagctgagaa aaaatccgaa gatgaacgat aaccataagc cccgtcgaaa
     1921 gacggggttt tattcatagt caatcttccc atttttttta gcaaggaaaa tttatgaatg
     1981 aaaaagagct tcgtatcatt atcgctagaa ttagaagtga aaaagaagta ttgctcacag
     2041 aatttcgtga gcttgcaagt caatttagtg taagagaatt ttctgaaaaa attgaggtcg
     2101 atcattctag aattcataaa attttgaatg gcaaattgga cgttagtatt gagactctct
     2161 gtgaatacat agagagattt ttgaaggtta aaaaatgaaa acttttttgt atattgtaat
     2221 ctatttggga gttgtaattt tccatttaat ggatgcttat ttagctgcaa ttgcttatgg
     2281 atttccattg ggtctttacc tttttttgtt ttccctttgt atcgttggag tgcttttaag
     2341 tttatggcga gattcaaagt tgctattagc cgcttgcttt ttagaattct ttcttgctgg
     2401 acttttggca atcggtaacg gaagaaattt atttgatctc gatttttctg ttttatttct
     2461 cccttctatt ttgcgaatca ttgtcctagt ccttcattac ttttctattt ttgaactctc
     2521 taaaattttt ctacaccgtg aacgctatta gctcatttcc taattccatt tctatttaaa
     2581 aactttccga gagcgcgagc gttggtcgga agatttaact cttggatgat ctgttttata
     2641 gtgtaacctt tgtcgcggag ggcttggaat ttctccaaat catcggctcc atattttgta
     2701 tttccatgtt cgaatttact taaaatttgc ttcattgttg tctctcctta aaatgttttt
     2761 ttgctttaca tattgtagat aaatgcgaaa ttataaacag ggattgtctt agcactttgc
     2821 taatagtaag ttgtaactga gtggcgtttt tcgccactag ccaaatatcc ttacgtgggc
     2881 tgtcatctaa tagtgtagca tctaggacgt tagcaactag gctagcgaat gtgagtgcaa
     2941 ttctcaccag cctcatccta ctacctctct ccttaaaatg ttttttgcgg cttctgtgag
     3001 gatgcgtgca tcgtttcttg taagtagttt ttcgcttttt ttaaactgcc tttgagacat
     3061 tccttcgagc gttaatttgt atatgccttg ggaatttatt tgagatagta aattgcttag
     3121 atattctctt tgttctctgg ttgcaatagc tactatattt ggattttttt tgctgcgtat
     3181 tgttttgtat ttgcgcgttg gggctggttt tttcggtagc tttgatcgta gagaacgaat
     3241 gaatttatct gcttctaaat ccgatagttt actgattgaa tcttttttac aaattaaatt
     3301 taaatgcaaa tacaactcct cttctgtgag ttgtaactct tttttgtaag caaaaatcgt
     3361 tcgtagtttt ttgggttcca tggtgtctcc ttgcgaacgc atgtatgcgt tccttactag
     3421 tttagctcgc agtgcatcgt tagttgtgtg ttgtgtgtca aaatgttttg tcgtttgatg
     3481 tagcgatagg ctttttggta gccaatccat gctgattttt ctttcaggat tttaattctt
     3541 aatccggtta gtttgtcgat gaagtcgtaa tcatcttgga attgggctac tggtttcatt
     3601 gggtctccat gttaggtaaa aacaattgag aattgtttgc cgcgattatt attataatac
     3661 acaaactttt cgtgcgtgaa tcgtctgacg gttgtaaaga gatttccttt agagtctcta
     3721 aaaagtaata ttagatgtag acctttgcca agtggataat tcgcaatagt ctttccatcg
     3781 tagtatgctg tgtcatactc tttgaattcg tcagaaaatt cctcggttgg cttttggaat
     3841 acttctacta attgaacagg atgatcctgt tcaatgtttc caagtttaaa atattcatga
     3901 ctgaatttaa tttggttcat atagcttttt cctgttctcg ttttttgatt ttcgcattta
     3961 atataacgta gtatttttta attcgctcgg agttttttag ctcgggtggt aagcggtcga
     4021 ttgttgatac ttgcgcgcca cgtctaaaga aatcctcgca atggttgatc cacatatcct
     4081 cattgtgtaa ctggattagt tctttcattg ttgcatcaaa ttcagattcg caatgaggac
     4141 atttcatcta agcaacctct aattctgtat ttgtagaata tggtttgata tagaaactct
     4201 cttccccatc ctctacatct actcccgtta ctactttcgc agtttccgga ttttgcaaaa
     4261 ttgcatcctt gtttaactca agcttggtcc ggataaatac cttatccatt tttttcacaa
     4321 acttctcttt tgcctcaatc agattgtttt gttcgaggat acttgtgatt agtttcacgc
     4381 ttgaattttg tgaaacatgt ttgcctgcag atctatacga taggtctccc gtctctaact
     4441 tcacagtctt ctcgccccct ttgaaaagct tctcgcgatt gttatcagtg aattttttca
     4501 cagataagga aatcttttta atctcttgtt tgagaggttc gatttgttct gctaaggttg
     4561 tttgtaagat agagatagat tcatttgttc tatcctctgc cttctttatc tctgtggtca
     4621 atcgtgctat ttcggctatc gcctttggta aatcgctttg ctcatactcg tagattacga
     4681 ttgtctctgt tggttgtggt tttgcttttg ccatgtttgg tctccttttt cgtaatgaag
     4741 tgaactacta catatttagt ggacacgatt ttaggcaaat tgcttctcat att
//
LOCUS       DAOQOS010000234         4787 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_11127_length_4787_cov_6.261200, whole genome shotgun sequence.
ACCESSION   DAOQOS010000234 DAOQOS010000000
VERSION     DAOQOS010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4787)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4787)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4787
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11127_length_4787_cov_6.261200"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(821..1204)
                     /locus_tag="PLF66_25740"
                     /pseudo
     CDS             complement(821..1204)
                     /locus_tag="PLF66_25740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002972570.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
     gene            1277..2443
                     /locus_tag="PLF66_25745"
     CDS             1277..2443
                     /locus_tag="PLF66_25745"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HMZ67518.1"
                     /translation="MIKREMLKKLLSVKKEYPVIALLGPRQSGKTTLARITFPQKKYI
                     NLEDLEVREFAQTDPRGFLSSYPDGCILDEIQRVPELLSYIQVFVDEDRKAGKYIITG
                     SQNLLLMEGISQSLAGRVALLSLLPLSLKEIFSNQKKIFSYEDYIFNGFYPFLYNNKK
                     INISSYYTNYIQTYIEKDVRSLKNISNLSSFTRFVKLCAARAGGILNLSSLADDAGIT
                     HNTAKSWISILEASYILFLLPPYFKNFGKRLLKSPKLYFFDTGILCALLGIKDVEQIS
                     THYLKGGIFENFVIMDLYKQFTNKGQIPPFYYWQDKHGKEIDLVIELGNKHLPIEIKS
                     GKTISTDFFNNLNHYKKIGDASVLKGCVVYGGDEIQKRTDADVFSWKNLSSLPF"
     gene            complement(2514..>4787)
                     /locus_tag="PLF66_25750"
     CDS             complement(2514..>4787)
                     /locus_tag="PLF66_25750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020775911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HMZ67519.1"
                     /translation="TKTRMLVQIFTLISASFTIYVLIRAILSKKQGAKIFLGGYLFAF
                     TIGVHDVLLSQYLIDSIPLSALGLFGFIFSQATVNSIQFSRAYASAENFGEELKAKSD
                     ELELLNKNLENKIAERTLDLAEAKSEVESLNRFTYLINSLSDLDAIFTEMSKYIYRKY
                     GIVATWLLLPDKNLENLNAYKVYSYKRLPEEKYQYLMNKKVPLSEEGGMLYVTYKRKK
                     TFYLSRIPKLKSNFDREISEIFNVESFLYVPLLSKNETIGIFCFSNLDFEMKLSRKET
                     KSISNLCSQVAGVVETAYLFEQVQEEKEKALVAQIETETQKRETENLNKLIKSLNEAL
                     DLKIIMEKVHHYVRKNFGIDFYALYNVNSEKTHIQILDVLFPDFVSEDTANIIRNLQI
                     PIKNVKGAHAFPFKAKKPLYISEIKSTGSTEEELYIMEKCKIQSFILIPLILKNESIA
                     IIDFFKFSEKLALTKEDIVRLSILGEQLAGIIHGSNLFKEVQEEKKNAEEARRQADID
                     KGIAILAQNEAEDERMKSEKLLLNIFPKDVAMELKEKGFADPALFENVSVMFTDFKGF
                     TKIAETLTPEELVKDLDTCFSQFDIITERYNLEKLKTIGDSYMCAGGIPKQNKTHAID
                     CVLAALEILDFMKILKKINEERGFSFWEVRLGIHSGPLVAGVIGKKKFAYDVWGDTVN
                     TASRMESSGTPGKINISGTTYELVKEFFDCEYRGEVPAKNKGMIQMYYVNRLKPEYST
                     DENGRTPNDRFWEVYGI"
BASE COUNT         1554 a          841 c          702 g         1690 t
ORIGIN      
        1 ctttgttata accttctaag cgagaataat cctcactaaa aattgaataa tttccagatg
       61 tgcaactccc acttccgcag caggattttt gattttgttc ctttggctgg tttgcaattt
      121 gtccatattt ctcttttaca atttctttga tttgttcggc agtttccata tttgtctcct
      181 ttttggactt tctctctttg aattttactt tttatatatt ttatatcgta aatatacgat
      241 aaattaaagt caagtgttta atgaaaaaat aaatatgata ataaacagta aaaaatgaca
      301 gagacggtat agtttactaa aacttgttct aattgacttt gtattttttc taaatgaatc
      361 caagcgatta aaaatatatt tactgatgac tagagaagtt ccatacatct ttataagacc
      421 ttccagatag atgggaagaa attcatcgca tctgctcaca gttctttcca tttttagatg
      481 aattagaaaa actattctta tataaatggg aggcattaac caagaaccca aacaataaga
      541 agaaacgact catttttttt aaatacgcac taactgatga attgctatag tggttcgtat
      601 agtgttgctt tagttatttg tatagtgaat aacgttttta gtatagcaag gactatagaa
      661 tccgcaaacc ttaaaaaaat atatggcaaa gcgaagtata tctgatttca taatgcactc
      721 aaagaaaaga cctgaaatat ccaagtaact aaatatagaa aacttgttcc tattttaaat
      781 ctcgaaggaa acaaaatcaa gctttgggaa agttagttat ttactcccca tacacttccc
      841 aaaatcgatc gtttggaatt ctaccatttt catccgtaga atactcaggc tttaatctat
      901 ttacataata catctgaatc atacctttat tcttagcagg aacttcccct ctctattcac
      961 aatcaaagaa ttcttttact agttcgtatg tcgttccact aatgttaatc ttacctggtg
     1021 tgccacttga ctccatacga gaggtagtgt tgactgtatc tccccaaata tcgtaggcaa
     1081 actttttttc tccaatcaca ccggcaacga gtggacccga atgtattcca agacgcagtt
     1141 ctcaataggg aaatcctttt tcttccttga ggcgtttcat cctgttctta aaatttttga
     1201 actcccccat aaaaaagcat actggacaat tttaatagtc gattgacaat ttgtccatta
     1261 tagatattct tgctgtatga ttaaacggga aatgctgaag aaacttcttt ctgtaaaaaa
     1321 ggaatacccc gttattgctc ttttgggtcc gagacaatca ggtaagacta cactcgccag
     1381 aattactttc ccacaaaaaa aatacatcaa tcttgaagat ttagaagtgc gagaattcgc
     1441 tcaaactgac ccaagaggct ttctaagtag ttaccccgat ggatgtattt tagatgaaat
     1501 tcaaagagtt ccagaacttt tatcctatat tcaagtattt gttgatgagg atagaaaagc
     1561 agggaaatat attattacag gttcgcagaa cttactttta atggaaggga tttctcaatc
     1621 acttgcaggt agagttgctc ttttgagtct tttaccttta agtttgaaag agattttttc
     1681 taatcagaag aaaatttttt cttatgaaga ttatattttt aatggctttt atccttttct
     1741 atacaacaac aaaaaaataa atattagttc ctattatacc aattatattc aaacctatat
     1801 tgaaaaagat gttcgtagtc ttaaaaatat ttctaatttg agttccttta cccgttttgt
     1861 aaagttatgt gcggcaagag cgggtggtat attaaatttg agttctcttg cggatgacgc
     1921 aggaatcaca cataataccg caaagtcttg gattagtata ttagaggcga gttatatact
     1981 ttttctatta ccgccttatt ttaaaaattt tggaaaaaga cttttaaaat ctcccaagct
     2041 gtattttttc gatacgggaa ttttatgtgc tctgctcggt ataaaagatg tagaacaaat
     2101 ttctacacat tatttaaaag gtggaatatt tgaaaatttt gttattatgg acttatacaa
     2161 gcaatttaca aacaaggggc aaatacctcc tttttattat tggcaggata aacatgggaa
     2221 agaaattgat ttagtgattg agttggggaa taaacattta ccaatcgaaa ttaaatcagg
     2281 aaaaacaatt tcaactgatt tttttaataa cctaaaccat tataaaaaaa ttggagacgc
     2341 ttcagtctta aaaggatgtg ttgtgtatgg cggtgatgaa attcaaaaaa gaacagacgc
     2401 tgatgtcttt agctggaaaa atttaagttc acttcctttc tagcaatttc aaaaaaataa
     2461 ttgcgctttt taatttaatt attaagctca ttgatggtaa ttctttatcc cccttatatc
     2521 ccatatactt cccaaaatcg atcgtttgga gttctaccat tttcatccgt agaatactca
     2581 ggcttcaatc tatttacata atacatctga atcatacctt tattcttagc aggaacttcc
     2641 cctctatatt cacaatcaaa gaattctttt actagttcgt atgtcgttcc actgatatta
     2701 atcttaccag gtgtgccact tgactccata cgagaggcag tatttacggt atcaccccat
     2761 acgtcatacg caaacttttt tttcccaatt acacctgcaa ccaaaggacc tgaatgaatt
     2821 cctaaccgca cttcccaaaa agagaatccc ctttcctcat ttattttttt tagtatcttc
     2881 ataaagtcta atatttctaa agcggccaat acacagtcaa ttgcatgtgt cttattttgc
     2941 ttgggaatac caccagcaca catatagcta tcgcctattg tctttaattt ttctaaatta
     3001 tatctttcag tgattatatc aaactgagaa aaacaagtat ctaaatcttt tactaattct
     3061 tctggagtta aggtttctgc aattttagta aatcctttga aatcagtaaa catcacactc
     3121 acattttcaa aaagagctgg atcagcaaaa cctttttcct tcaattccat tgcaacgtct
     3181 ttaggaaaaa tattaagtaa taacttttca gatttcatgc gctcatcctc tgcttcgttt
     3241 tgcgcaagaa tagctatacc tttatcaata tcagcttgtc ttcttgcctc ttctgcattt
     3301 ttcttttctt cttggacttc tttaaaaaga ttcgatccat gaataattcc agcaagttgt
     3361 tcaccaagaa tagaaagacg aacaatatct tcttttgtaa gagccaattt ttctgaaaat
     3421 ttaaaaaaat ctatgattgc gattgattca ttttttagaa tgagtgggat tagaatgaac
     3481 gactgaattt tacatttttc cattatatat agctcttctt ccgttgagcc ggtagacttt
     3541 atctcactaa tatagagtgg cttctttgcc ttgaaaggaa aagcatgtgc tcccttcaca
     3601 ttcttaattg ggatttgcag attacgtatg atattagctg tatcttccga cacaaaatct
     3661 ggaaacaaaa catcaagtat ttgaatatga gttttttctg aatttacatt ataaagagca
     3721 taaaaatcaa ttccaaaatt tttacgaaca taatgatgaa ctttctccat gattattttc
     3781 aagtctagag cttcatttaa acttttaata agcttgttta gattttcagt ctctcttttt
     3841 tgtgtttcag tttctatctg agcaactaac gccttctctt tttcttcctg aacctgttca
     3901 aataaataag cagtctcaac gactccggct acttgagaac aaagatttga aatactttta
     3961 gtttcctttc tggaaagctt catttcaaag tctaaattcg aaaaacaaaa aattccaatg
     4021 gtttcgtttt tgcttagtag agggacatac aaaaaggatt ccacattgaa aatttctgaa
     4081 atttctctat caaaattaga ttttaatttt ggaatcctag aaagataaaa tgtctttttt
     4141 cgtttatagg ttacatacaa cattcctcct tcttcagaaa gagggacttt tttattcatt
     4201 aaatactgat atttctcttc tggcaatctc ttataactat ataccttata agcattcaaa
     4261 ttctctaaat ttttatcagg taacaatagc caagtggcca cgatcccata ttttctataa
     4321 atgtattttg acatttctgt aaaaattgca tctaggtcag aaagagagtt gattaaataa
     4381 gtaaaacgat ttaaagattc tacttctgat ttggcttcag cgaggtctaa cgttctctct
     4441 gcaattttat tttctaaatt cttgtttaat aactctaatt catctgattt ggcttttaat
     4501 tcttcgccaa aattttcagc cgatgcatac gctcgtgaaa attgaattga attcactgtt
     4561 gcttgtgaaa aaataaatcc aaataatcct agagcggaaa gtggaatcga atcaataaga
     4621 tactgagaaa gtagaacatc atgaacgcct atcgtaaaag caaataaata tcctcctaga
     4681 aatatttttg ctccctgttt tttactgaga attgcacgaa ttaaaacata gattgtaaaa
     4741 ctagctgaaa ttaaagtaaa gatttgaact agcatacgag tctttgt
//
LOCUS       DAOQOS010000235         4741 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_11313_length_4741_cov_7.216389, whole genome shotgun sequence.
ACCESSION   DAOQOS010000235 DAOQOS010000000
VERSION     DAOQOS010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4741)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4741)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4741
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11313_length_4741_cov_7.216389"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            717..1379
                     /locus_tag="PLF66_25755"
     CDS             717..1379
                     /locus_tag="PLF66_25755"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - oxidoreductase activity, acting
                     on NAD(P)H, nitrogenous group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HMZ67520.1"
                     /translation="MEVKEAILNRKSVRQFLPKEVEKEKLDRLMDLTRRAPTWKNAQG
                     YKIAIVQGKMKDQISNDFMQAIESGTPENPDYPYQDFYPHYMKKRMLELGSSYYGHLG
                     VDRKDKERRKELLLDNFKFFGAPVGIFFLMERGMNFWPTLDLGILVGTLLIAARDEGL
                     ETIAQASLAAFPDIVRKNLNLETNWTVAVGVSLGYGDPNALTNQFVSPRVDKTEIIQF
                     YE"
     gene            complement(1360..2538)
                     /locus_tag="PLF66_25760"
     CDS             complement(1360..2538)
                     /locus_tag="PLF66_25760"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HMZ67521.1"
                     /translation="MNYLSDRFDFERSENELSRILELTKNNADFLDLTISNPTSLFHY
                     DLEKIQKGLNASFLFPYSPDPFGIESAREILVKYYQSKGRIFQKENFFFASGTSEAMS
                     FILKAIANRDSEVLLPTPGYPLYDFILKLENVKTEYYALLPRKVSKNQLLWEIDFSLL
                     EQKINKNTKALVIVEPQNPTGARLTKSDAERLVEVIRKNKLILIIDEVFSDYYKDYYL
                     SNPYKNINCIYLNGLSKTLALPQMKLSWIYLSGEAEFISQMKESLEIICDTYLSVNSM
                     VQYGLGFLLETAVDIQSQIINQIQVNLELVERVLMGQDLIEWFKPEGGWYLLLRINNQ
                     WKDEDFSIQLLKEKHVYILPGYMFDIHEEGYIVISLIVKSNILEEGLNRILAFIHKTG
                     "
     gene            2955..3923
                     /locus_tag="PLF66_25765"
     CDS             2955..3923
                     /locus_tag="PLF66_25765"
                     /inference="COORDINATES: protein motif:HMM:NF025931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat protein"
                     /protein_id="HMZ67522.1"
                     /translation="MYYFSIIFFLILNIQLLSAEDAKSDRTALAEVLKNAKISQTDIK
                     NVSNLDKMELGDENQISYDPVSGRIIGLKLHLTEFNDLSLLAPMTGLKVLIVSGDEGG
                     GQINQLNGLENLSNLKILSLREHKIQKIKGLQNLKNLEKLDLSMNEISKIEGLDHLKN
                     LKKLELGDNKLTKIENLNSLTYLEELYLHRNQITKLENLDFLVNLRILHVYENEMAKI
                     ENLDNLRNLEELELGVCKFQRIEGLNNLTKLKILKLWYNKITRIEGLDSLVNLETLDL
                     SNNKISKIEGIDNLPEIKFVDLTNNRITNKKLVKKYLNRNIMIELE"
     gene            complement(3943..4476)
                     /locus_tag="PLF66_25770"
     CDS             complement(3943..4476)
                     /locus_tag="PLF66_25770"
                     /inference="COORDINATES: protein motif:HMM:NF017015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HMZ67523.1"
                     /translation="MQTLPTIYPCKNKEDLAKQVIEVHKSIAEFYRSIPDEYFSGDAI
                     PEGWTVRRNMKHVISTNGSFGFWIGLPKFIFKIWRKPSAKQPTIEQLEPTNRRGITYY
                     GKYEKNQANKEEKEKLLELINASAAKVASQIQKRTEEELDQFAGVFGGMSLRTFCYFL
                     MKHNVHHTNVVRLRLEN"
BASE COUNT         1671 a          791 c          717 g         1562 t
ORIGIN      
        1 tttagtctcc ttttggaagc ctaaaatcgt gtcctttttt tatgtagggg ttcacccaag
       61 ccagttagtc gccaggtggt agcttcttgg tctcctagtt ggtagccaga tagtataacg
      121 ttatccataa ctgaatacga agaaaataag aaaaagacta ccaccgatgg gcaccgataa
      181 cggatacgat atgtttttag attcgattgg ctattggtta ttagccacat aataatatat
      241 tagctgaatc attttataat agcaagcttt catagattta aattaaaact cctcggtggc
      301 aatctacacc ttctcaaaag tggaagatta gacccaatcc cctaaaaaat cataatattt
      361 tttgattata atctaaaaat attatgattt tttttagatt taaatggaaa tgctgccaag
      421 aaagatttcg ggtagatcaa tagggtttta tcattagccg agatacaaag aatatagatc
      481 ccaaatggag aatcctgcag gttcatgcta aaatgtcgca gtttcgggtt tcttttcttt
      541 cttgcatact tttttcttta caaaaagaaa agaaaaaagt atgaagaacc ctcgctggaa
      601 agcagaaaaa ggaaaaaatc tttttttaat atttgatgga atcaacgtaa cttgacaata
      661 tcctattcta atacaattta aaaactaatt ctaaatcccg cataaggaac gaaacgatgg
      721 aagtaaaaga agcaatatta aaccgcaaat cagttagaca attcttaccc aaagaagtag
      781 aaaaagaaaa actagatagg ctaatggatc ttacccgcag agcacctaca tggaagaatg
      841 cgcaaggtta taaaatagca atcgtccagg gaaaaatgaa agatcaaatt tccaatgatt
      901 ttatgcaagc cattgaatcg ggaactccgg aaaatccaga ttatccgtat caagacttct
      961 accctcatta tatgaaaaag agaatgttag aacttggatc ttcttattat ggtcacctcg
     1021 gagttgatcg aaaagataaa gagagaagaa aagaattgct tttggataat ttcaaatttt
     1081 ttggagctcc tgttggaatc ttctttctaa tggaacgtgg catgaacttt tggcctacac
     1141 tcgatctagg aattttagtt ggaactttat taattgctgc tcgtgatgaa ggtctagaaa
     1201 caattgcaca ggcaagtctt gcagcctttc cggatatcgt tcgaaaaaat ctaaatctag
     1261 aaacgaattg gactgtagct gttggagtat ctcttggtta tggtgatcca aatgcactta
     1321 caaatcaatt tgtctcccca agagttgaca aaacagaaat tatccagttt tatgaataaa
     1381 cgcaaggatt cgatttaacc cttcttccaa tatgtttgat ttcacaatga ggctaatcac
     1441 tatataaccc tcttcgtgaa tatcaaacat atatcctggt agaatataaa cgtgtttttc
     1501 tttcaatagt tgaatggaaa aatcctcatc tttccattga ttgtttattc ttaataataa
     1561 ataccaacca ccctccggtt taaaccactc aattaaatcc tgtcccatta acactctttc
     1621 gaccaactcc aaattgactt gaatctgatt tataatttga ctctgtatat caactgccgt
     1681 ttccaaaaga aaacccaaac catattgaac cattgaattt actgacaaat aggtatcaca
     1741 aataatttct aaagattctt tcatctgact tataaattca gcctcaccag ataaatagat
     1801 ccaagataat ttcatttggg gaagagccaa tgttttagaa agcccattca aataaataca
     1861 atttatattc ttatacggat tgcttagata atagtcttta tagtaatcag aaaaaacttc
     1921 atctataatc aatattaatt tgttttttct tataacttcc acaagtcgct cggcatccga
     1981 tttagtcagt cttgcacctg tcggattttg aggttctaca atcacaaggg cttttgtgtt
     2041 tttatttatt ttttgttcta acaaagaaaa atcaatctcc caaagcaatt gatttttact
     2101 tacttttctt ggcaataaag cataatactc tgtctttaca ttttctaatt ttaatataaa
     2161 atcatagagc ggataaccag gagtaggcaa taaaacttct gagtctcgat ttgcaattgc
     2221 ttttaaaata aaactcattg cctccgatgt gcctgatgca aaaaagaaat tttctttttg
     2281 aaaaattctg cctttcgatt gataatattt cacgagaatt tccctagcag attcgattcc
     2341 aaatggatcg ggggaatagg gaaataaaaa ggaagcattg agtccttttt gaattttttc
     2401 cagatcataa tgaaacagag aagttggatt cgaaatggtt aaatctagaa aatctgcatt
     2461 gtttttcgta agctctaaaa ttctagacag ttcattttca cttctctcaa aatcaaatcg
     2521 atcggataaa taattcataa tcaatggaaa gaggggacat aaataattag caagtagttt
     2581 tttgtttgga tcaaatctat ttcttatcta ttctctctaa taaatttcta ttatcagaaa
     2641 ccttttaata cacacaattg cacatctgat tcaatttgaa tcatttttta tatactagat
     2701 tcttacttta ccaaacaaga cactaaatct aatgttattc acatctgatt tctatcctaa
     2761 aataaaacat gacttttatc attgtttcca aagaaagaaa tctctctttt tactaattca
     2821 acatttaatg attgcaaaat attagtagtc tatttaaata tcttacccga ttgaaaagac
     2881 ctcttagcga aaaatgaacc aattcatttg gtaagtatat gaaaattatt cttcttttca
     2941 ggagccatca tagtatgtat tatttttcta ttattttttt tcttattcta aacattcaat
     3001 tattgagtgc tgaagatgct aagagcgata gaactgctct cgctgaagtt ctgaagaatg
     3061 caaagatttc acaaacagat ataaaaaatg tttctaattt agataaaatg gaattaggcg
     3121 atgaaaatca aatctcctac gatccagtat caggaagaat cattggactc aaactacatc
     3181 ttaccgagtt taacgactta tcactattag cccctatgac gggactaaag gtattaatcg
     3241 tttctggtga tgaaggtggt ggtcaaatta atcagctaaa tggtttagaa aatttatcca
     3301 acttaaaaat cctttcttta agagaacata aaatccaaaa gatcaaaggt ttacaaaatt
     3361 taaaaaactt agaaaaatta gatttaagta tgaatgagat ttctaaaatt gaaggacttg
     3421 atcacctaaa aaatctaaaa aaactcgaac taggtgacaa taaacttaca aaaattgaaa
     3481 atctaaatag cttaacttat ttagaagaac tctatctcca tcgaaatcaa attacaaaac
     3541 tagaaaattt agacttccta gtaaatctta gaatacttca tgtctatgaa aatgaaatgg
     3601 ctaagattga aaatttagac aatctaagaa atttagaaga actagaacta ggtgtttgta
     3661 aatttcagag gatagaaggc ttaaacaatc taaccaaatt aaagatttta aagttgtggt
     3721 ataataaaat tacaagaatt gaaggactcg attcgctcgt gaatttagaa acattagatc
     3781 ttagtaataa taaaatctct aaaattgaag gaattgataa cctacctgaa attaagtttg
     3841 tggatctaac aaacaatcgt atcacaaaca aaaaacttgt taaaaaatat ttaaacagaa
     3901 atataatgat agaattagag taaaggctaa atgcctttac ctttaatttt cgagcctaag
     3961 tcgaacaacg tttgtatggt gcacgttatg cttcattaag aagtagcaaa atgttctaag
     4021 actcatacct ccaaatacac cagcaaattg atctaattcc tcttctgttc ttttttgaat
     4081 ttgagaagca actttcgcag cagaagcatt gatcaattct agcaatttct ccttttcttc
     4141 tttgtttgcc tggtttttct catacttacc ataataagtg ataccacgac gattcgttgg
     4201 ctctaattgc tctatagttg gttgtttggc tgaaggcttt ctccaaattt taaaaataaa
     4261 ctttggaaga ccaatccaaa acccaaaact accatttgtg ctgataacat gcttcatatt
     4321 tcttcggact gtccaacctt ctggaattgc atctccggaa aaatactcat ctggtatgct
     4381 gcgataaaat tctgcaattg atttatgaac ttctatgact tgcttagcaa gatcttcttt
     4441 gtttttacat ggataaatag ttggtaatgt ttgcacaata ctaagaaatt ttacgagtca
     4501 gaaatatgca aatcgttttt ttatcagtcg tttttccttt tttgattgaa aagcttaaat
     4561 aatagcaaag agtattctta tgcttcaact actctggaat caaatcgctt atatgggtgt
     4621 tcaacatgtt ccaaacatta cagaacacaa acatgtagtt ttagtaaata cactatcttt
     4681 gttcatacca gtatttactt ttctaaatgg ttttaggaaa caagaagtag tgaatgtcct
     4741 c
//
LOCUS       DAOQOS010000236         4717 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_11404_length_4717_cov_7.061347, whole genome shotgun sequence.
ACCESSION   DAOQOS010000236 DAOQOS010000000
VERSION     DAOQOS010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4717
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11404_length_4717_cov_7.061347"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     repeat_region   2..903
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=2..38
                     /rpt_unit_seq="ctaacatccaattacaacaacgacctgcaactgaaat"
     gene            1098..1304
                     /locus_tag="PLF66_25775"
     CDS             1098..1304
                     /locus_tag="PLF66_25775"
                     /inference="COORDINATES: protein motif:HMM:NF021560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2283 domain-containing protein"
                     /protein_id="HMZ67524.1"
                     /translation="MQVTYDSTTDLLYIRIDSASHQITNKRITDDIVLDLGEDGKIVG
                     IEILDASKQVNLSSLLPISIKQVA"
     gene            1493..1744
                     /gene="cas4"
                     /locus_tag="PLF66_25780"
     CDS             1493..1744
                     /gene="cas4"
                     /locus_tag="PLF66_25780"
                     /inference="COORDINATES: protein motif:HMM:TIGR04328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type V CRISPR-associated protein Cas4"
                     /protein_id="HMZ67525.1"
                     /translation="METYIPISFLNDFIFCPRSIYFHQVHGGMSQELYSSKEQTEGRL
                     AHEAIDEGTYSTKKKVLMGTEVYTEKNNTSTKVWNSLGI"
     gene            1756..2373
                     /gene="cas1"
                     /locus_tag="PLF66_25785"
     CDS             1756..2373
                     /gene="cas1"
                     /locus_tag="PLF66_25785"
                     /inference="COORDINATES: protein motif:HMM:TIGR00287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas1"
                     /protein_id="HMZ67526.1"
                     /translation="MENQTLLLKSIRGKTIDVKTSIADLEKYKNTEMTELNLKTILGT
                     EGISSRVFFKIWFADLDWKGRKPRAKIDKTNVILDIGYTYLFNIMEGMLNLYGFDVYK
                     GIYHQSFYQRKSLVCDLVEPFRCIVDRKVKNAYALGQIKEEDFTESKGQFFLKIDQSK
                     KYTSWLVQEIMKYKEEIFLYTQSYYRAFMRNKATSDYPLFHIRED"
     gene            2373..2645
                     /gene="cas2"
                     /locus_tag="PLF66_25790"
     CDS             2373..2645
                     /gene="cas2"
                     /locus_tag="PLF66_25790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010413876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HMZ67527.1"
                     /translation="MLLISYDISDTKLRTKFSKYLLKYGERLQYSVFEIKNSERVLVN
                     IETQIKQYFEKRFSQDDSIMIFHMSPSCKITRYGYAKNQERDVLIF"
     gene            2726..3034
                     /locus_tag="PLF66_25795"
     CDS             2726..3034
                     /locus_tag="PLF66_25795"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HMZ67528.1"
                     /translation="MIISSNDSLEKIKAILRNHKEEIFKKYSVREIGIFGSYIRGAET
                     KESDLDILVDFQKPIDLFSFLELEEFLSDLCKCKIDLVSKKTLKPYIGKQILSEVIYL
                     "
     gene            3031..3381
                     /locus_tag="PLF66_25800"
     CDS             3031..3381
                     /locus_tag="PLF66_25800"
                     /inference="COORDINATES: protein motif:HMM:NF014043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HMZ67529.1"
                     /translation="MKRIFLDSLQDILDSINDIESFTQSIDFEKFLLNRLVQNAVVRS
                     LEIIGEASKNIPDETKEKYADVPWRRIAGMRDKLIHNYFGVDYEMVWEVAKNKIIELR
                     PSILQMINDLKNEE"
     repeat_region   3525..4059
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=3525..3559
                     /rpt_unit_seq="tcgaagagaggaaataaatttctactattgtagat"
     gene            4278..4427
                     /locus_tag="PLF66_25805"
     CDS             4278..4427
                     /locus_tag="PLF66_25805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67530.1"
                     /translation="MEKKEESILKKKGYMTGKEFKEAVKKSSYLKKILNKLDKAVDKE
                     FKEDA"
BASE COUNT         1841 a          730 c          784 g         1362 t
ORIGIN      
        1 tctaacatcc aattacaaca acgacctgca actgaaattg ctcttgagta ggtgcaactt
       61 tattccctga gaaactaaca tccaattaca acaacgacct gcaactgaaa tctcattgct
      121 ccgttgccag ttcctgaata ggttgctaac atccaattac aacaacgacc tgcaactgaa
      181 atctaatcgc acagaggaaa ataagacttg atttttcgct aacatccaat tacaacaacg
      241 acctgcaact gaaatatgat actgagaggg tcaatgaatt atactcacag ctaacatcca
      301 attacaacaa cgacctgcaa ctgaaatcta ttggcaaatt tgataaaact tgctgttaat
      361 tctaacatcc aattacaaca acgacctgca actgaaataa aatccgaaca tgtaaaaact
      421 acatctaatt taactaacat ccaattacaa caacgacctg caactgaaat ttcgatcctt
      481 acccgacaga ttttatatgc ctctttctaa catccaatta caacaacgac ctgcaactga
      541 aatacaaata cgacagccgc tttaggatct gggttaggtc taacatccaa ttacaacaac
      601 gacctgcaac tgaaattagc aacattaacc acaacgggca atcaaaaaaa ctaacatcca
      661 attacaacaa cgacctgcaa ctgaaatttc agtcacctta gaaataaggg aagaaaataa
      721 tactaacatc caattacaac aacgacctgc aactgaaata actaggttgt tttaacgtta
      781 gtcgtaaatt aacctaacat ccaattacaa caacgacctg caactgaaat acacatatta
      841 tgtgatctat gtgcttcata tgctagctaa catccaatta caacaacgat gcatggaagt
      901 gcggtcacgt gaccgcgcct gcgttgtatc gaccgcccaa ttacaataat tcccccatca
      961 aaataaaata gaagctttaa aaatttcaat acgataaaaa tagttgttga aggaaagaat
     1021 tccagtctta aatattttcc tatataaaaa tatttagcca atcattacat aaataataat
     1081 gatgtgcgag gaatttaatg caagtgactt atgattcaac aactgattta ctttatattc
     1141 gaatcgattc tgcatcacac cagattacaa acaaaagaat cacagatgat attgtgttag
     1201 atttaggtga ggatggaaag attgttggaa tcgaaatttt agatgcttca aaacaagtca
     1261 atctatcctc tctccttcct atttcgatta aacaagtagc gtgatttttc tataccaatt
     1321 cccaaaagta aattccgatt ggaaactaga aaaccgtctt ttgggaattg ggaatacccc
     1381 ttttgtgaaa aatagcagaa cttatttatg agaagtggta tataagcaag gctacttgtt
     1441 cgttgcaata gaatattttt cctttaattt cttttactaa ccaaactaac gcatggaaac
     1501 ctacatccca atttccttcc taaacgactt catcttctgt ccacgttcta tctatttcca
     1561 ccaagtgcat ggtggaatga gccaagaatt gtatagttca aaagaacaga ctgagggaag
     1621 gcttgctcat gaagcgatag acgaaggaac ctattcgact aaaaagaaag ttttaatggg
     1681 aacagaagtt tatacggaga aaaacaatac cagtacaaaa gtctggaact cgcttggcat
     1741 atagtaaaaa ataaaataga aaatcaaaca ctacttttaa aaagcattcg gggaaagacg
     1801 atagacgtta agacaagcat tgcagactta gaaaaatata agaacacgga aatgactgag
     1861 ttaaatctaa aaacaattct aggaactgaa ggaatttctt cgagagtgtt ttttaaaatt
     1921 tggtttgcgg atttggattg gaagggacga aagccaaggg cgaagattga taagacaaat
     1981 gtgatactcg acattggata cacgtatctt tttaatatta tggaaggaat gttaaacttg
     2041 tatgggtttg atgtttacaa aggaatttat catcaaagtt tttatcaaag aaaatcttta
     2101 gtttgtgatt tagtcgagcc atttcggtgt attgttgatc gaaaggttaa gaatgcgtat
     2161 gcgctcggac aaattaaaga ggaagatttt acggaaagca aggggcagtt ttttctaaaa
     2221 atagatcaaa gtaaaaaata cacaagttgg ttagtccaag agattatgaa atacaaagaa
     2281 gaaatttttc tttatacaca atcctattac agagcgttta tgagaaataa ggctacttcg
     2341 gattatccgc tctttcatat acgggaggat taatgttact tatctcttat gacatcagcg
     2401 atacaaaatt aagaacaaag ttttccaagt atctattaaa gtatggagaa agattacaat
     2461 actctgtttt tgaaattaag aatagtgaaa gagtgcttgt aaatatagag acacagatta
     2521 aacagtattt cgagaaacgt ttttcacaag acgatagtat aatgattttt catatgagcc
     2581 ctagttgtaa aatcactcgc tatggatacg cgaagaatca agaaagagat gtgttgatct
     2641 tttaatttac gaatcaagga tcataagaaa aaatagagtt gagtttttga gtgaatgctg
     2701 ttgaagttga ttctagagag aagatatgat tatatctagt aatgacagtc ttgaaaaaat
     2761 caaagcaatc ctacgaaatc ataaagaaga aatttttaaa aagtattctg tacgagaaat
     2821 tggaattttt ggatcttata ttcgtggagc agaaacaaaa gaaagtgacc tagacatatt
     2881 agtagacttt caaaaaccaa tagacttgtt ttcattttta gaattagaag agttcttatc
     2941 agacttatgt aaatgcaaaa tagatttagt ttctaagaaa acattgaaac cgtacatcgg
     3001 aaagcaaatt ctatcagaag taatttactt atgaaacgaa tatttttaga ttctttacaa
     3061 gatattttag attcaataaa tgacattgaa tcgtttacac aatctataga cttcgagaaa
     3121 tttttattaa atcgattggt tcaaaatgct gttgtaagaa gcttagaaat tattggagaa
     3181 gcttcaaaga atattccaga tgaaactaag gaaaaatacg cagatgtacc ttggagaaga
     3241 attgcaggaa tgcgagataa gttaattcat aattatttcg gggttgatta tgaaatggtt
     3301 tgggaagtag caaagaataa aattatagaa ttaagaccaa gtattcttca aatgataaac
     3361 gatctaaaga atgaagaata aatttttgct tgcaaacctc cgtctgaatg aaaagaatta
     3421 gaagaaagta ggaaaaaaga atggttaaat gcttacaaga taagcagaat tcgaatcaaa
     3481 ccgaagaaaa aagccaaaaa acctgcttaa ttcacaaaat accctcgaag agaggaaata
     3541 aatttctact attgtagatt atttaattgt gtccatacat tacttttcga agagaggaaa
     3601 taaatttcta ctagtgtaga ttaaaagaaa ttgcttttaa attccatgtc gaagagagga
     3661 aataaatttc tactattgta gatgagcact agaaaagatt ttaaatcctt atcgaagaga
     3721 ggaaataaat ttctactatt gtagatatgc tatcatattc taatcagtat gactcgaaga
     3781 gaggaaataa atttctacta ttgtagatag ttgtagcccg ttataaacgt agaagctcga
     3841 agagaggaaa taaatttcta ctattgtaga tatagacgct ccctttcaac acctgttact
     3901 cgaagagagg aaataaattt ctactattgt agatatagac gctccctttc aacacctgtt
     3961 actcgaagag aggaaataaa tttctactat tgtagattat tatctctttg ggtaagataa
     4021 acactcgaag agaggaaata aatttctact attgtagata tgctatgttg acactcttct
     4081 aaagtctcga tgagaggaaa taaattttga acgcgagcct acggaggctt gcctattttg
     4141 tccaatcata atctagatgt atgcggtata taaatttata ttcaatcctg atattcctct
     4201 catgtctgaa attttagaac aagaaaagaa ggtaaaaaaa tagtttagtt ttataaaata
     4261 ggagaaaggg aaaaaacatg gaaaagaaag aagaaagtat tttaaagaaa aaaggatata
     4321 tgacaggaaa agaatttaaa gaagctgtga aaaaaagttc ttatcttaaa aagattttaa
     4381 acaaactaga taaagctgta gataaggaat ttaaagaaga tgcatgaaaa tgatcctgtt
     4441 tttatacatc taacatccaa ttccaataat tcacccatcg aaaaaaaata gaagctataa
     4501 aaatttcaat acgataaaaa taatccttga aggaacagat tccattctta aatattttaa
     4561 tataaaaata ttacacaaat catcacatga aataaacata gatataacaa agtagaagga
     4621 attaaatgca agtagattat gattcaagaa ctgatttaca atatattcgg aataattttg
     4681 gatcacagca aattaacata aaagattatt tcgatat
//
LOCUS       DAOQOS010000237         4637 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_11762_length_4637_cov_7.721082, whole genome shotgun sequence.
ACCESSION   DAOQOS010000237 DAOQOS010000000
VERSION     DAOQOS010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4637)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4637)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4637
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11762_length_4637_cov_7.721082"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..932)
                     /locus_tag="PLF66_25810"
     CDS             complement(<1..932)
                     /locus_tag="PLF66_25810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67531.1"
                     /translation="MNSIKNISIFFILSIFTSLMADTIIFSDGRVLEDVKVKIRKDTV
                     EVLYKNGKREVRKKTEFKKSKIKLIPVAWGSDSDFDSKKSSSSSQNVESEIAELERQI
                     EANFAIPPKLLDERRRHDFLMKERIRLQYTEGLLTYAGGQQFYTEIMQKNNDRYFVRN
                     QFGLLFFNRLDFGDEMVIETDNGKEVIDISKIQAVKDEKFIKGFIYLSGAEKIKGTIK
                     KNLGDQIVIETLKGELRVPASDVLFPPLPPIEKNTEKRLTIKQGEAGNFLLRNGDTIS
                     GKLVRSDKTYLIIETEYGMIEIEESNVISFTKGNP"
     gene            complement(1168..2910)
                     /locus_tag="PLF66_25815"
     CDS             complement(1168..2910)
                     /locus_tag="PLF66_25815"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015679595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HMZ67532.1"
                     /translation="MSKPLIVQSDKTLLLEVDNPEFEDCRAIISRFAELEKSPEYLHT
                     YRISPLSLWNAASSAMTADMIVEGLEKFARYSIPKNVVNEIREQISRYGKVKLVKEET
                     GELVIISNEKGFLQEIANHRAVQPFIQERLNNEKIVVKKEYRGHIKQALIKIGFPVED
                     LAGYDEGNKYGFNLRPITREGKKFGMRDYQRASIEVFHAGGRNEGGSGVVVLPCGAGK
                     TIVGMGVMQIIGAETLILVTNTLSIRQWKNEILDKTDIPESDIGEYSGETKEIKPITI
                     ATYNILTHRKRKGSDFTHFHIFSANNWGLIVYDEVHLLPAPVFRMTSELQAKRRLGLT
                     ATLVREDGLEEDVFSLIGPKKYDVPWKELENKSWIAEAKCVEIRVPMDEELRLRYSIS
                     DDREKFRLASENPEKMSAIELILERYKDSHILIIGQYINQLETIAEKFKIPLITGKTP
                     LGERQELYTAFRSGAIKSLVVSKVANFSIDLPDANIAVQVSGTFGSRQEEAQRLGRIL
                     RPKVGNNTATFFSLISRDTSEERFGQNRQLFLTEQGYEYEIYTLDQFQETGVQTEDHR
                     YPAQSIAEPMITGV"
     gene            3095..>4637
                     /locus_tag="PLF66_25820"
     CDS             3095..>4637
                     /locus_tag="PLF66_25820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HMZ67533.1"
                     /translation="MKEEFKQRLLKFKMRFWLYAEIIGVCLIAPTVVIFAITFIDLDN
                     GQAKIIAISSFIVVLLLFLIAAIVNARIISPFIAYLELAAEGKTAPDAIYSNAQKSFN
                     HIPKIHSLSIFIRWVIGLTIVTSAVYFFSHARIAQITNLIGVGFFGGVLNALWSFSLN
                     EFLNIKIAETGIFNQDANNQRENYSKLFLSLAIQIGSAMLLLSTAILIVSFNLNSKSL
                     SQAFENQMNNINESNVQVLDAFYNAKEQDITKFSSDPEIQKLVQDKNWSSISQYLSLY
                     LLNSKNYYEGTFVFTLDSDYTVVATSSTKGEAVGLKLKEVPNMQKNIQEVLNGNLFFG
                     EIQASPITFAAKVVVTAPIKVNGKIVAGVGFAFLVGEFANEIIRNVNIGTDGYPFFVT
                     KNLTVIAHKNNKKILKSLKEEPYSLYLQDTEDQKSYIFSSDGKIRFIRKQISSKYDFI
                     AISTALQDDLEKPALSSIRNIGILSFLTLIATGFFLYHLLNLKLGPLAESSKALLKME
                     NGNLSE"
BASE COUNT         1467 a          871 c          699 g         1600 t
ORIGIN      
        1 ggattcccct ttgtaaaaga gatgacattc gattcttcta tttcaatcat cccatattct
       61 gtttcaataa ttagataagt tttatcagat cggactagct tgcctgaaat tgtatccccg
      121 tttcgcagaa gaaaatttcc tgcttctcct tgcttgatag tcagtctttt ttctgtgttt
      181 ttttcaattg gaggcagagg tggaaacaaa acatcacttg ctggcacacg taactctcct
      241 ttaagagtct ctattactat ttgatctcct agattctttt taattgtgcc tttaattttt
      301 tcagcacccg ataggtagat aaaacctttt ataaattttt catctttgac tgcttgtatt
      361 tttgaaatat caattacttc tttcccatta tctgtctcaa ttaccatttc atctccaaaa
      421 tcaagacgat taaaaaaaag aagtccaaat tgatttctta caaaataacg gtcattattt
      481 ttctgcatta tctctgtata aaactgctgc ccacccgcat acgttagtag accttctgta
      541 tattgtagtc tgattctttc tttcatcaga aaatcgtgac gtcttctctc gtccaaaagc
      601 tttggaggaa ttgcaaaatt tgcctcaatt tgtctttcta actcggctat ctctgactca
      661 acattttgac tgctactaga actttttttt gaatcaaaat cggaatctga tccccaggca
      721 accggtatta atttaatttt gcttttttta aattcagttt ttttacgaac ttcccttttc
      781 ccatttttat atagaacttc taccgtatct tttctaattt ttactttcac atcctctaaa
      841 actcttccat ccgaaaagat aatagtatct gccattaatg aagtaaaaat ggataaaatg
      901 aaaaatatac tgatgttttt gattgaattc aagatagtct cctaaattta gaggtaaaat
      961 aaaatttgcc gcacccattt agcttttacc aattcttatg aaatacacat cattcaaaag
     1021 agcaaagaat cggtttttaa aatttatcaa attagattgt gataaaataa aaaagatgtc
     1081 gcattactgt aaataaagga ttcaaagtcg cgacatccat ttagtaagct tccatagcta
     1141 gaaaagcttt ttgataatcc ttttaggtta tactccagtg atcataggtt ctgcaatcga
     1201 ttgagccgga taacggtgat cttctgtttg cacgcctgtt tcttggaatt gatctaaagt
     1261 ataaatttca tattcataac cctgctctgt taagaataat tgtctattct gaccaaatct
     1321 ttcctcactt gtatctcttg aaatgagaga gaaaaaagta gcagtattat ttcctacttt
     1381 tggtctaaga atacgaccga gacgttgtgc ttcttcttgt cttgatccaa aagttcccga
     1441 tacttgcaca gcaatattag catccggtaa atcaatagag aagtttgcaa ccttacttac
     1501 tactagtgat ttaatcgcac cagatctaaa agcagtgtaa agctcctgtc tttctcctaa
     1561 cggtgttttt ccagtaatca aagggatttt aaatttttca gcaattgtct ctaactgatt
     1621 gatatattgt ccaatgatca aaatatgtga gtctttatat ctctcaagaa tcagttcaat
     1681 tgctgacatt ttttctggat tttcggaagc gagtctgaat ttctcacgat catcagaaat
     1741 agaatagcgt aaacgaagtt cttcatccat aggaacacga atctcaacgc attttgcttc
     1801 tgcaatccaa gatttatttt ctaattcttt ccaaggaacg tcatattttt taggaccgat
     1861 taaactaaat acatcttctt caagaccatc ttctcgaact aaagttgctg taagcccaag
     1921 acgacgcttt gcttgtaatt ctgacgtcat acgaaacact ggagctggta ataagtgaac
     1981 ctcatcatat acaatgagtc cccagttatt tgcgctaaaa atatgaaagt gagtgaagtc
     2041 agaacctttt ctctttcgat gagttagaat attataagta gcaattgtaa ttggtttaat
     2101 ttcttttgtt tcaccagaat attctccaat gtctgactca ggaatatctg ttttgtctaa
     2161 tatttcattt ttccactgac gaatcgaaag agtatttgtt acaagtatca aagtctctgc
     2221 tccaataatt tgcattacac ccattccaac aattgtctta cccgccccac aaggaagaac
     2281 gacaactcca gatccacctt cgtttctgcc acctgcgtga aagacttcga tagatgctct
     2341 ctggtaatca cgcattccaa actttttccc ttctcttgtg atcggtctaa gattgaatcc
     2401 gtatttattt ccctcatcat aacccgctaa atcttccaca ggaaatccta ttttgataag
     2461 agcttgcttg atatgacctc ggtattcttt ttttacaacg atcttttcat tgtttaatct
     2521 ttcttgtata aaaggctgaa cagctctgtg gtttgcaatc tcttgcaaaa atcctttttc
     2581 attggatata attactaatt ctcctgtttc ctcttttact aattttactt ttccataacg
     2641 actgatttgc tctctaattt cgtttacaac attcttagga atggagtatc ttgcaaattt
     2701 ttcaagacct tccacgatca tatcggctgt cattgcagag gaagctgcat tccataaaga
     2761 caaaggggaa attctatagg tatgtaaata ttcagggctc ttttcaagct cagcaaagcg
     2821 agatataatc gctctacaat cttcaaattc gggattatct acttctagta aaagtgtttt
     2881 atcactctgg acaatcaatg gtttactcat atactactcc ttaaaaaggc acgtacagag
     2941 tggaaactac catccatctg tcaagcataa aatagcccca ttttttcgag ccaaggtttt
     3001 taaaataacc aaaattgaga ttcatgaaag ggcttgctag tttctagact aaaagaattt
     3061 agtgaaataa atccacctat ttactgagtt caatatgaaa gaagaattta aacagagact
     3121 attaaaattt aaaatgagat tttggttgta tgctgagatc attggagtgt gcttgatcgc
     3181 acctacagtt gtcatctttg ctattacatt tattgattta gataatggac aagcaaaaat
     3241 catcgcaatc tcttccttta tagtagtcct cctccttttt ctaattgcag caatagtaaa
     3301 tgcaagaatt atatcgccct tcatcgccta cttagaatta gccgctgaag gaaaaacagc
     3361 tcctgatgca atctattcaa acgcacaaaa aagttttaat catattccca agattcattc
     3421 tctttctata tttattcgtt gggttatcgg acttaccatt gtcacgagtg cagtttattt
     3481 tttttctcat gctagaattg cacaaattac aaatttgatt ggggttggtt tcttcggagg
     3541 cgttctaaat gctctttggt ctttctcctt aaatgaattt ctaaatataa aaattgcaga
     3601 aacaggaatt tttaatcaag atgcaaacaa tcagagagaa aattacagta aactatttct
     3661 atctctagca attcaaattg gatctgcaat gcttcttctt tcgacagcca ttctaatcgt
     3721 ttcttttaat ctaaattcta aatctctttc acaagcgttt gaaaatcaaa tgaataatat
     3781 caatgaaagc aacgtgcaag tattggatgc tttctacaat gcaaaagagc aggatataac
     3841 gaaattttca tcagatccag aaattcaaaa actagtgcaa gataaaaatt ggtctagtat
     3901 cagtcagtat ctttcactct atctattaaa ttcaaaaaac tattacgaag gaacctttgt
     3961 atttacactt gattcagact atacagtagt cgcaactagc tctaccaaag gagaagcggt
     4021 aggactcaaa ctaaaagaag ttccaaatat gcaaaaaaat attcaagaag ttctaaacgg
     4081 gaatttgttt tttggagaaa tacaagcatc tcctattact tttgctgcta aagttgttgt
     4141 aaccgcacca atcaaagtga atggaaaaat tgtagctggt gtaggttttg catttttagt
     4201 tggagaattt gcaaatgaaa tcattcgaaa tgtaaatatt gggacagatg gttatccttt
     4261 tttcgtaaca aaaaatctaa cagtcattgc tcataagaac aataaaaaaa tcctgaaaag
     4321 cttaaaagaa gagccctatt ctctctattt gcaagataca gaagatcaaa aatcatatat
     4381 cttttcgagt gatgggaaaa taagatttat cagaaaacaa atcagcagta aatatgattt
     4441 catagctatt tcaacagcct tacaggacga tctagaaaaa ccagctctct ctagtatacg
     4501 aaacattggt attctttctt ttttaacgtt aattgcaaca ggattttttc tttatcattt
     4561 attaaattta aaactaggac ctttagcaga aagttccaaa gctttattga aaatggaaaa
     4621 tggaaatcta tcggaag
//
LOCUS       DAOQOS010000238         4502 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_12345_length_4502_cov_6.769732, whole genome shotgun sequence.
ACCESSION   DAOQOS010000238 DAOQOS010000000
VERSION     DAOQOS010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4502)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4502)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4502
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12345_length_4502_cov_6.769732"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            300..671
                     /locus_tag="PLF66_25825"
     CDS             300..671
                     /locus_tag="PLF66_25825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67534.1"
                     /translation="MKSHSNIKIVKRILLQFFLSILMVISLFNSVSESLQCLQSDLLG
                     VDEEYDFRIHSQKQQNQFYIFNSFSILEDDNEEYEYLTSSFSAQKNELKINLVNYFYL
                     TTHFNTWIRPYSECCLPPPLI"
     gene            923..1687
                     /locus_tag="PLF66_25830"
     CDS             923..1687
                     /locus_tag="PLF66_25830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67535.1"
                     /translation="MKPKKEDSKMGYNDLYVDKETGQVFITPGNNRVKLDRKTDSKSN
                     SITTLPDAFAHRPDDLSKEKLTIAGRIQYRGASGSAESPLSNGHRDFNTMGFAFRRLR
                     LGAMYENDWWGTNIQLRLENMVNRVDIAQTTQTINYTDSTGKPASATFVTNERMKDNR
                     GYIQEAVAYAKLPYAGARVTVGQINVPFNREYIGSSANLVALERSMVTAALPQFDNGV
                     MLTATPLKEINPKYERYLHLSLMVGNGRGGGGDYGT"
     gene            1847..2518
                     /locus_tag="PLF66_25835"
     CDS             1847..2518
                     /locus_tag="PLF66_25835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67536.1"
                     /translation="MKLSLGAASQQTENLVTPAVSAMEYNSGTTNGIAFVTPQGANGY
                     PSADRGNVTTVNGMLQPVFNVQNGGTTPGRPKLGLVAHTYDATFTWNGFYFNGAYTKM
                     IGAASNGLIGWHTTFGYNIPISKFYIMPVFKYDQLVGDFNRNGTKHDPTDTLKIYWLG
                     LNLFGDKHHYKLQVYYEILANKLNRDPNTGNAMMIDDRRVYFQVQANFWTGTDSLESY
                     SYRSN"
     gene            2530..3009
                     /locus_tag="PLF66_25840"
     CDS             2530..3009
                     /locus_tag="PLF66_25840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67537.1"
                     /translation="MKQFFYLIFILVILFDCTQTKKRNTNGLSALIVNNALNGTATDK
                     YTNESLLDLRRADLANLSFTGVCYDSFTFNTIPVSPTNYYNTPAGGYGGKINTDIEKH
                     TLSTSNCSSLGFSPASNTSFGSSTQRPSSDTSFNYKMYSCDPNNNPCSAAAIKAAGF"
     gene            3242..3616
                     /locus_tag="PLF66_25845"
     CDS             3242..3616
                     /locus_tag="PLF66_25845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67538.1"
                     /translation="MRFPEIKRYVLNLLLSFFIFIITIDVFSEPIEIILDNISTPSIE
                     SNTRLLQKSSHFDESEFIFIYTACKSLDEDDFEEYLYNTSRFIFFGIFYLNSFRKSLD
                     IANLWFKKYISFCEYNIPPPSV"
     gene            3979..4293
                     /locus_tag="PLF66_25850"
     CDS             3979..4293
                     /locus_tag="PLF66_25850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67539.1"
                     /translation="MIEKKRRIKKYAIREWSEYQGQMNWYEANKISEELGMRLPTIEE
                     LKFAFQTKETSSWKKNGKCYWTCDENDYSKNYAFAFFIDSGIIFDYDKTINLDVRFTK
                     DR"
BASE COUNT         1553 a          680 c          796 g         1473 t
ORIGIN      
        1 ctcacccaaa ctcaatcatt gcttttgtta agcaatgata atgtaaggtg agttactcaa
       61 attagatctt tttaaatatt tccaaaaatc aaaacagcaa atgaagaatt ttttcatttc
      121 ttaaagaact tgtaatcaaa ttgtgaagat tcggttttat tctcttatta aaacattact
      181 atatataaat agagtttata caatagttaa tcatggtata tattctgtaa ttctttcggt
      241 gcatatgcag atttatagct ggtattttta attagatagg acttagaatt ttcagaaaaa
      301 tgaaatccca ttccaatata aagatagtaa aaagaatctt acttcagttc tttttatcta
      361 ttttgatggt gatttcatta tttaattctg tcagtgaatc gcttcaatgt ttacagtcag
      421 atctcttagg agttgatgag gaatatgact ttcgaatcca ttcccaaaaa cagcagaatc
      481 aattttacat ttttaattct ttttccattt tagaggatga taatgaagaa tatgaatatc
      541 ttacatcttc tttctctgca caaaaaaacg aattaaaaat caatcttgta aattattttt
      601 acctgacgac gcattttaat acctggattc gaccttactc tgaatgctgt ttaccacctc
      661 ctctcattta aaacaaaaaa acagcaaaag gtttttatgg ctattggaat cactctgtga
      721 atctaactag tcagattcat tcttttttga tggtgcgtta aaatttataa cgataagcga
      781 aacgcataag gatgaattat tgcatcaaaa gaaaattcaa tttaaattag aggaaaaatg
      841 aaatcatctc tcaataaaat attatattat ctaataactt ttgttttaat ttcaagcagt
      901 gtatccattt tactagctca ggatgaaacc aaaaaaagaa gactcaaaaa tgggatacaa
      961 tgatttgtat gtagataaag aaacgggtca ggtctttatt accccgggca ataaccgtgt
     1021 gaaattagat agaaaaacag attcaaaaag taattcaata acaactctac ctgacgcatt
     1081 tgctcataga cctgatgatc taagcaaaga aaaactaacc attgcaggaa ggattcaata
     1141 taggggagcg agtggtagcg cagaatctcc cttatccaat ggacatagag attttaatac
     1201 aatgggtttt gcatttagaa gactacgact tggtgctatg tatgaaaatg attggtgggg
     1261 aacaaatatc caacttcgct tagaaaatat ggtaaatcgt gttgatatag cacagacaac
     1321 acaaacgatc aactacaccg attcaacagg aaaaccagca agtgctactt tcgttacaaa
     1381 cgaaagaatg aaggacaata gaggttacat tcaggaagct gtggcttatg ccaagttgcc
     1441 ttatgcggga gcaagagtaa ctgtcggtca aataaatgtt ccttttaatc gagaatatat
     1501 tggctcttct gccaatttag ttgcactaga aagaagtatg gttaccgctg cacttcctca
     1561 atttgataat ggtgttatgt taaccgcaac tccattaaaa gagatcaatc caaaatatga
     1621 acgttattta catctatctt tgatggtagg aaatggtcgc ggtggtggcg gtgattatgg
     1681 aacttgaaga agacaggatc taacaacggc taaccgctat ggatttgtaa atatctctcc
     1741 tacctattat gcacgtgcag tatggaatgt gttcggaagt ttaaaaagag atacagatgg
     1801 aagagaagta aattggcaag agggagaaga aatattccaa cgagatatga aactctcact
     1861 tggagcagct tctcaacaaa ctgaaaatct tgtaacacct gctgtgtctg caatggagta
     1921 taattctgga actacaaatg gaatcgcttt tgttactcca caaggtgcaa atggatatcc
     1981 gtccgctgac agaggcaatg taacaacggt aaacggaatg ttgcagccag tttttaatgt
     2041 tcaaaacgga gggacgactc ctgggcgacc aaaacttgga ctcgttgctc atacatatga
     2101 tgctacattt acatggaatg gtttttattt taatggggct tatacaaaga tgattggtgc
     2161 tgcgtctaac ggtttaattg gatggcatac tacctttggt tataatattc caatcagtaa
     2221 gttttatata atgcctgttt ttaaatatga tcaattagtt ggtgatttta atcgaaatgg
     2281 aactaagcac gacccaactg atactctgaa aatttattgg ttaggtttaa atttatttgg
     2341 tgataagcat cattataaac tgcaagttta ttatgaaata ctcgcaaata aattaaatag
     2401 agatccaaat actggaaatg caatgatgat tgatgatcgc cgtgtctatt ttcaagtgca
     2461 ggcaaatttt tggacaggaa ctgattctct tgaatcgtat agttatagat caaattaaag
     2521 gataacctta tgaaacaatt tttttattta atttttattt tagtcatact ctttgattgc
     2581 acgcaaacta aaaaaagaaa tacaaatggt ttaagtgctc tcattgtaaa taatgctttg
     2641 aatggaacag ctacggataa atatacgaac gaatcattat tagatcttag aagagctgat
     2701 ctggcaaatc tatctttcac aggtgtatgt tatgattcat ttacattcaa tactatccct
     2761 gttagtccaa caaattatta taatactccg gctggtggct atggtggaaa aatcaatact
     2821 gatattgaaa aacatactct atctacttcg aattgtagta gtttaggatt ttccccagca
     2881 tctaatacta gctttggatc tagcacacaa cgtcctagct ctgatactag ctttaattat
     2941 aaaatgtata gctgtgatcc aaacaataat ccgtgttctg cggcagcaat taaagcggca
     3001 ggattttagt aggagaaaaa atgctgaaag actttgaatc aattgagtct gttggcattg
     3061 atttgatttc ataaactagt tttataatat ggatagaaaa aagtatttag gctagatcgt
     3121 gttagtaaaa atactttcac atacttgcga ttttttgcag gtagattttt taagactatt
     3181 caattcagtt ggaaatgaga ttttttattg accattgttt taaagattac gaatctaggc
     3241 catgaggttt cctgaaataa aaagatatgt attaaatctt ctgttatctt tttttatttt
     3301 tatcattacg attgacgttt tcagtgaacc gatagaaata attttagata atatttctac
     3361 tccctctatt gagtcgaata ctcgcttgct tcaaaaaagt tctcatttcg atgaaagtga
     3421 atttatattt atttacactg catgcaaaag tttggatgaa gatgattttg aagaatattt
     3481 atataacact tcaagattta tattttttgg aattttttat ctgaattcat ttagaaaaag
     3541 tttagatatt gcaaatcttt ggtttaaaaa gtatatctca ttttgtgaat acaatattcc
     3601 tccaccctct gtttaaaaat ctattatgtt ttcctttatc aaatttattt tcagctgaat
     3661 actgagtatc aaaagtattc aaaataaatg cgatagttta taacctaaat attttacaat
     3721 taacgaattg ttggaataga tttaggcgaa atggatatct cgaaaaaggt aaagtattta
     3781 taactcagct tacgctaaat ttataaatta ggaataaacg atgattttta taatattaga
     3841 taattctaat gaatgaacaa aatcaatagc ctgcgatagc tcgactgttg gagagcttat
     3901 aaggcgatgg gtgggcaaac cctccgttcc ttctcttttc ctattcgggg tcagttagaa
     3961 tgaaatcagg tgattaagat gattgagaaa aaaagaagaa taaaaaaata cgcgattaga
     4021 gagtggagcg aataccaagg acaaatgaac tggtatgaag caaataaaat atcggaagag
     4081 ttagggatga gactcccaac gatagaagaa cttaagttcg ccttccaaac aaaagaaacg
     4141 agcagttgga aaaaaaatgg taaatgctat tggacttgtg atgaaaatga ttactccaag
     4201 aattatgctt ttgctttttt tattgatagc ggtattatct ttgattacga taagacaatc
     4261 aatctagacg ttcgtttcac aaaagataga tagatttaga gattattttt aactgatttt
     4321 gaatgagaag acttgctaat ttttaaaacc ctctctaggc atagaaaaag cgtggatttc
     4381 tccacgcttt ctaaaatcaa aagtcgaatt gtttagactt ctgaagattt tctgctgatc
     4441 cagaaacgtc ctgcaagtat tgctcctact aagatggcga ttgcagcagc tgctgctcga
     4501 gc
//
LOCUS       DAOQOS010000239         4327 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_13214_length_4327_cov_5.795880, whole genome shotgun sequence.
ACCESSION   DAOQOS010000239 DAOQOS010000000
VERSION     DAOQOS010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4327)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4327)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4327
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13214_length_4327_cov_5.795880"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..440
                     /locus_tag="PLF66_25855"
     CDS             <1..440
                     /locus_tag="PLF66_25855"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HMZ67540.1"
                     /translation="GFHFPFTCANCSQITYSNPIPVTVLLVPVEKGILVVRRGIEPAK
                     GQLALPGGFIETGETWQEAGVRELREEADVSISPDSVKEFMVQSANENRLVLIFGVTR
                     ELKKEELTPFIPNAETLERKIIYAYEELAFELHSLALSKWFQK"
     gene            complement(537..1880)
                     /locus_tag="PLF66_25860"
     CDS             complement(537..1880)
                     /locus_tag="PLF66_25860"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HMZ67541.1"
                     /translation="MKEKEVRYQLAKELARYFLYHLKEELKKPEAVTEYITMEGVPFK
                     KIIQDMIQNLVKEFPDLDNKLPTFNLFHHWWKRTIKEIGTDSSKPYEESTFKSQNQMF
                     FFKIIDIKKKESLLRRRYEENMEASIRGLRAKGRNIGFETIFRSEYIMFPLIELIIRR
                     SRLPYRSCLNILLEQNFFIQGIAYRIFDSELKESFKKTDDILDSILPHNIVDELKLKG
                     KVEPKLIPSASILFCDLVGFTNISTNLPPEKLLSELDGCYSHFDKIVKMKGLEKIKTI
                     GDSYMAAGGISSNGRLHAIDAILSALKIQEFLRKYEKSQERKGLPSWKVRIGIHTGQV
                     VCGILGEQRFNFDIWGDTVNTAQRMEAYGEAEKINVSKEVYEDTKEFFNFESRGKIQV
                     KGKGDMEMYFVTGIKENLSISQKGKTPNSRFLQLYLSHLNNDSLMKEFLTTDKHR"
     gene            1940..2974
                     /locus_tag="PLF66_25865"
     CDS             1940..2974
                     /locus_tag="PLF66_25865"
                     /inference="COORDINATES: protein motif:HMM:NF022427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2804 domain-containing protein"
                     /protein_id="HMZ67542.1"
                     /translation="MKAKKIENHAQEKLIQANGMPSFGIFNHPINEVNFKDYKYLNPF
                     GKIKNSISKHFAYNQFEYVGGVSDKIIFGVAIADLKFLTNAFFYIYLPETGKFLHKSL
                     KQPFSIGSKFSNFPETGVVSFSKGKNKIEMAPLNGRRKLLLELSSGETIDAEFLEEKI
                     EPLRICTRAGVNGWVYVRKTAGSPVVGKIECSLGKFDLEKLGVLGHNDYSVGFMRRET
                     FWNWTCFTAKINSQIISVNLSCGVNETSYSENSLWIDGVRYPLSQVAFQYDKENIKKE
                     WKVFSLDKSIDLTFIPEGNYKENINAYILASNFNQLYGKYFGKLQLPNKKFIKLDGIY
                     GYAEDHYAKW"
     gene            2996..3388
                     /locus_tag="PLF66_25870"
     CDS             2996..3388
                     /locus_tag="PLF66_25870"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HMZ67543.1"
                     /translation="MNEEIDLTAEDFNYSDKQITISVRTIGVPENLPENGVVIDLKGI
                     LNLYSANPLKSVLSNLINVGKIRIYVYMKHLENIDSSGIGGLIAVQTKLSKMNGVLKV
                     LEPSDKTRSVLKLTNLETYFHMSEKFVP"
     gene            complement(3390..3707)
                     /locus_tag="PLF66_25875"
     CDS             complement(3390..3707)
                     /locus_tag="PLF66_25875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67544.1"
                     /translation="MADIEITNPNTIQEIVYESGRFKYILSEIYNLKISAIQSIRLDS
                     DNFKIDINTKDTYYRFKPKGFEDVLMTNPETRSMELKFDPALAQAVYRILNDCMYEEV
                     KQD"
     gene            complement(3749..>4327)
                     /locus_tag="PLF66_25880"
     CDS             complement(3749..>4327)
                     /locus_tag="PLF66_25880"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HMZ67545.1"
                     /translation="SKLAGKGKPFFITGFSLGGNFALRIAKKNSKKLIPNLKHAVGIS
                     PAIHPKSSTEMMDENIFLRRFFLKQWMKSLNEKEKLFPFLYDFKKYKDAKSVMNLTER
                     IIPDYSPYKSSDEYFETYTLTKDYFKDLKTPVTIITAKDDPVVHPEDFYELTPNPNLD
                     VRIEDYGGHNGFFNSFGLDCYYLPVLLEIFSK"
BASE COUNT         1393 a          679 c          730 g         1525 t
ORIGIN      
        1 atggttttca ttttcctttc acctgcgcaa attgtagtca aataacgtat tctaatccaa
       61 ttcctgttac agttttactt gtgcctgttg agaaagggat tttagttgtg agaaggggaa
      121 tagagccagc aaaaggacag ttagcactac cgggtggatt tattgaaaca ggggagacat
      181 ggcaagaggc aggggttaga gaactaagag aggaagcaga tgtttccatt tcacctgatt
      241 cagtaaaaga atttatggtg caaagtgcaa atgaaaatcg acttgtatta atctttggag
      301 tgacgagaga attaaaaaaa gaagagctaa caccgtttat ccccaatgca gaaaccttag
      361 aaagaaaaat tatttatgcc tatgaggaat tggcttttga gctacatagt cttgcccttt
      421 caaaatggtt tcaaaaataa aagaatacca cctgtgaaga cgataagcac cgataaaaag
      481 gaagaaagat aaaatccaaa cttaatggtg cctccatagg tctcaacata atgtgttcat
      541 ctgtgtttat ccgtggttaa aaattctttc atcaaactat cgttatttaa atgcgataaa
      601 tacagttgca agaaacgaga attcggagtt tttccttttt ggctaatgga aagattttct
      661 ttgattccag taacaaaata catttccata tctccttttc ctttgacttg aattttccct
      721 cttgattcaa aattaaaaaa ttcttttgta tcttcgtaaa cttcctttga tacatttatt
      781 ttttctgcct ctccgtaagc ttccattctc tgtgctgtat ttacagtatc tccccaaata
      841 tcaaaattaa atctctgctc ccctaaaatt ccacaaacga cttgtccagt atgaattccg
      901 attcgaacct tccaactagg taagcctttt cgttcttgac ttttttcata ttttcttaaa
      961 aattcttgga tctttagtgc tgatagtatt gcgtctattg catgaagacg accattcgag
     1021 ctaattcctc cggctgccat atagctatca ccgattgttt ttattttctc aagtcctttc
     1081 atttttacaa ttttatcaaa atgggaatag caaccatcta gttctgataa aagtttttct
     1141 ggtgggagat tggttgatat attagtaaag cctaccaagt cacaaaataa aatggatgca
     1201 gaaggaatta attttggttc gactttccct tttaatttta gttcatctac gatattatgt
     1261 ggtaaaatcg agtctagtat gtcgtctgtc tttttaaaag attcttttag ttctgaatca
     1321 aatatacgat aggcaatgcc ttgaataaaa aaattctgtt ctaaaagtat atttaaacaa
     1381 gagcgataag gcagtcgact tcttctaata atgagttcga tgagtggaaa cattatatac
     1441 tctgatctaa agatagtttc aaagccaatg tttctaccct ttgctcttag ccctctaata
     1501 gatgcttcca tattctcctc ataacgtctt ctaagaagtg attctttttt tttaatatca
     1561 atgatcttaa aaaagaacat ttgattttgt gatttaaacg ttgattcttc ataaggttta
     1621 gaactatcag ttccaatttc ctttattgtt cttttccacc aatgatgaaa aagattaaat
     1681 gtagggagct tattatctag atcaggaaat tcttttacta ggttttgaat catatcttga
     1741 atgatctttt taaaaggcac accttccatg gtaatatatt cagtgactgc ttctggtttt
     1801 tttaattcct cctttaaatg gtagaggaaa tatcgagcta attctttagc taattgatac
     1861 ctaacttctt tttctttcat actgtttgca gtattaattt atccttgctt aatgctttca
     1921 taatttcaat ttggtagctg tgaaagcaaa aaaaattgaa aatcatgcgc aagaaaaatt
     1981 aattcaagcg aatggaatgc ctagctttgg aatctttaat caccctatta atgaggttaa
     2041 tttcaaggac tataaatact taaatccctt tgggaaaatt aagaactcca ttagtaagca
     2101 ttttgcttat aatcaatttg aatatgttgg cggtgtatct gataaaatta tttttggtgt
     2161 agccattgct gatttaaaat ttttgacaaa tgcttttttt tacatttatt tacccgaaac
     2221 tggtaaattc cttcataaaa gtttgaagca gcctttttca attggttcca aattttccaa
     2281 tttccccgaa accggtgtag tatcattctc caaaggtaaa aataaaatcg aaatggctcc
     2341 tctgaatgga aggagaaaac tacttttaga attatcatca ggtgaaacaa ttgatgcaga
     2401 gtttttagaa gaaaagattg aacctttacg aatctgcact agggcgggtg taaatggttg
     2461 ggtctatgtt cgaaagacag caggaagtcc cgttgttgga aaaattgaat gctcattggg
     2521 gaaatttgat ttagaaaaat taggagtctt agggcataac gattacagtg taggttttat
     2581 gagaagggaa acattttgga attggacttg ttttaccgct aaaattaatt cgcagataat
     2641 cagtgttaac ctctcttgtg gtgtcaatga aactagctat tctgaaaatt cactttggat
     2701 tgatggtgtt cgttatcctc tttcacaagt tgcttttcaa tacgataagg aaaatattaa
     2761 gaaagaatgg aaagtctttt ctttagataa atcgatcgat cttactttta ttccagaagg
     2821 caattacaaa gaaaatatta acgcatatat actagcatct aactttaatc aactttacgg
     2881 aaaatacttt gggaaacttc aacttccgaa taaaaaattt attaaattgg atgggatcta
     2941 tggttatgcg gaagatcatt atgcgaaatg gtaaaatagt ttaggataat tttttatgaa
     3001 tgaagaaata gatttaacag cagaagattt taattattcg gataaacaaa tcactatatc
     3061 ggttcgaacg ataggtgtcc ctgaaaattt accagagaat ggagtcgtga tcgatttaaa
     3121 aggaattttg aatttatatt cagctaatcc attaaaatca gttttatcaa atctgatcaa
     3181 tgttggtaaa ataagaattt atgtatatat gaaacaccta gaaaacattg attcatctgg
     3241 aataggtgga ctcattgcag ttcaaacgaa gttatctaaa atgaatgggg tgttaaaagt
     3301 tctggaacct tctgacaaaa caaggtctgt tttaaagctg actaatttag aaacttattt
     3361 tcatatgagt gaaaagtttg ttccataggt taatcttgct tgacctcttc atacatacaa
     3421 tcatttaaaa ttctataaac tgcttgtgct aatgcaggat caaattttaa ttccatactt
     3481 cttgtttctg gattagtcat aagcacatcc tcaaagccct ttggcttgaa tcgatagtag
     3541 gtatcttttg tattgatatc tattttgaag ttatcgctat ctaatcgaat ggactgtata
     3601 gcgcttatct ttaagttata gatttcactc aatatatact tgaatctccc agattcgtat
     3661 acaatttctt gaattgtgtt tggatttgta atttcaatat cagccataga gtcataattg
     3721 aatcctattg aataaatttg tcaataaatt atttcgagaa tatttccaaa aggactggta
     3781 aatagtaaca atctagcccg aatgaattaa agaagccatt gtgtcctcca tagtcttcta
     3841 ttctaacgtc taggttagga ttgggggtca gttcgtaaaa atcctctgga tgaacgactg
     3901 gatcatcttt tgcagtgata atcgttacag gagttttcaa atctttaaaa tagtctttcg
     3961 taagtgtata ggtttcaaag tattcatcag aacttttata aggactataa tctgggataa
     4021 ttctttctgt taaattcatt acagattttg catctttgta ttttttaaaa tcatacagga
     4081 aaggaaatag cttttctttt tcgttaagag atttcatcca ttgcttcaaa aaaaatcttc
     4141 ttaagaatat attttcatcc atcatttcag tgctagattt cggatgaatg gcgggactta
     4201 ttccgactgc atgttttaaa tttggaatta attttttact atttttcttt gcgatacgaa
     4261 gagcaaaatt tccaccgagt gaaaaaccag taataaaaaa cggttttcct tttcccgcaa
     4321 gtttaga
//
LOCUS       DAOQOS010000240         4241 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_13610_length_4241_cov_5.170330, whole genome shotgun sequence.
ACCESSION   DAOQOS010000240 DAOQOS010000000
VERSION     DAOQOS010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4241
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13610_length_4241_cov_5.170330"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..387
                     /locus_tag="PLF66_25885"
     CDS             <1..387
                     /locus_tag="PLF66_25885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67546.1"
                     /translation="VAAFFGANYGKYRFYNGKSLVGVVSFIIASCILGILFMILFQNT
                     KENSIFAFTLGNAINYRALFAVILAAIVSGLTEFFSGHAWGGFLDDNLTIPLAAAFTL
                     AVVFVLFFGMNTEEIFFPVSNLFAHE"
     gene            complement(391..>4241)
                     /locus_tag="PLF66_25890"
     CDS             complement(391..>4241)
                     /locus_tag="PLF66_25890"
                     /inference="COORDINATES: protein motif:HMM:NF016944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004352 - glutamate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0019551 - glutamate catabolic process to
                     2-oxoglutarate [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase"
                     /protein_id="HMZ67547.1"
                     /translation="DLNADDPLLRINLHCIIIVCKSMPFFAGKIRSLFHKTNLPINRS
                     LQFHPEPGKEFYYIEIQTKNKDLIEKIIAEITKSYSKISNLTTQFPNFIDKLNSLSAV
                     TISDKWMLIDWLLKKGLVWEAGVIGDKDNKLELFGSAEETMQYVEWFKRLQNDSNELV
                     LECLETKDEGFLGDTPLFCIAFVQKNIKVLLIGSFTIHAQTLGVIEIPFFNKRFRAFL
                     DREKVEYSSGLGRSVRRIFNSFPIEVLFLVPESAYISIFKVIVEQSLKTENRSNGVIL
                     NSELSIILTTIPEKNWSETKLAETEKIVETVLKKAKIKTYHILLTNSIQCYHLIRSEN
                     ISKQNLFEISSQFEFLFRPWVEFIKTKWEDRFKDLPFPNGVEFRNDYIATHDPEKALY
                     DLELVQRLENKKVIFNITETKSNATLIEAVTLEKEFNLSKWVTVLTAFGLSAISQRVY
                     RFQCAGKTYSKTEFFFDLFENKDHLYDRLKNALRYTMEGVLPVDSLSSLILITNLDAS
                     ALLFLKSIREYCLQTNPSFNKSDFNEIMVQYPEFSTEIWKYFYSKFREGKPISDSRLK
                     ELSDQAKTIREDEVLNSIRTAVIAILRTNFFGTKFESNFGKESGLQRLAVAYKIDSSI
                     PVSLPLPRPFREIFVYSSLFQGIHLRGGSVARGGLRFSDRPSDFRTEILSLMKTQMVK
                     NTVIVPVGSKGGFVLSKNQFTSELPMVEAYKAYVYSLLSLTDNRKGSEKILFSDMNGP
                     FAYDEFDPYLVVAADKGTAQLSDTANAISLKHNFWLGDAFASGGSRGYSHKEYGITAR
                     GALVTADRQLRNLDIDYLKDIITVVGIGDMGGDVFGNGLIQSKAFKLVAAFNHKHIFL
                     DPNPDPEKSFEERKRLFFSNASGWDNYNPSLISRGGGVYLKTEKSIPISEEMKLVLGI
                     SDSTLSGSKLISALLKAPVDLLYNGGIGTYIKSEAEENADVGDPSNNDVRINGSEIRA
                     KVVSEGGNLGFTQLGRIEYALNGGNIYTDALDNSAGVDLSDHEVNLKIFFNHLKETGK
                     IKSEDERDLVLKQIVKPVCDSVLMDNALQSLAINVDYYESIYKGWENFIKGSQNLISK
                     KILNPETEKIPTTLLEWEDWKRQSKGIPKPALCVLLAYAKMEIYNESIQENLFTIDEF
                     SHFYYDYFPNDLSNQYKNELKEHPLKMEILNTQIINFYVNLLGFSGLLLLGEGNTRER
                     MLYFKEIVEYLYSLRIDMVLKEIASLREKSLESENVRLITEIRDRVRFKWGQSKKISL
                     EGTPIDSYLTENTKRNISKV"
BASE COUNT         1336 a          727 c          629 g         1549 t
ORIGIN      
        1 gttgcagcct tttttggagc aaattatggg aaatatagat tttataatgg taaatcgctt
       61 gtcggggtag tatcatttat aatcgcctct tgtatattgg gaattttatt tatgatttta
      121 tttcaaaaca caaaggaaaa ttctatcttt gcatttactt taggtaatgc tatcaattat
      181 agggctttat ttgccgttat attggcagct atcgtatcag gtttgactga atttttctca
      241 ggacatgcgt ggggaggctt tttagatgat aatttaacaa ttcctctcgc agccgcattt
      301 actctcgcag tagtatttgt tctatttttt ggaatgaata cagaggaaat atttttccct
      361 gtatccaatc tttttgctca tgagtgaata ttagacttta ctaatatttc ttttggtatt
      421 ctcagttaag taggagtcaa ttggggtgcc ttctaaggaa attttcttag attgccccca
      481 cttaaatcta actctatctc ttatttcagt aatcagtctt acattttcag actccaaact
      541 tttttctctc aagcttgcaa tttcttttaa taccatgtct attcttaagg agtataagta
      601 ttcaacaatc tccttaaaat aaagcattct ttctctcgta ttgccttcac ctaacaataa
      661 taaaccacta aaccccaata gatttacata gaaatttata atttgagtat ttaggatttc
      721 catttttaaa ggatgttctt ttagttcatt tttatattgg tttgataaat catttggaaa
      781 gtaatcgtaa taaaaatgag aaaactcatc aatagtaaaa aggttctcct gtattgattc
      841 gttataaatt tccattttcg catacgcgag taatacacac aaagcaggct ttggaattcc
      901 tttcgattgt cttttccaat cctcccattc caacaaggta gtaggtattt tttcagtttc
      961 aggatttagt atcttttttg atattaaatt ttgcgatcct ttaataaagt tctcccatcc
     1021 tttatatatt gattcgtaat agtctacgtt aatcgcaaga ctttgtaatg cattgtccat
     1081 tagaacagaa tcacaaactg gttttacaat ttgctttaga actaaatctc tttcatcctc
     1141 tgatttgatt ttaccggttt cctttaaatg attaaaaaat atttttaaat ttacttcatg
     1201 atcggagagg tcaactccag cggaattatc taaagcgtca gtatagatat ttcccccatt
     1261 taatgcatat tctatcctac caagctgagt aaatcctaga ttcccacctt cacttacaac
     1321 ttttgctctt atttcagaac cgttaattct tacatcattg ttagaaggat ctccgacatc
     1381 tgcattctct tctgcttctg atttaatata agttccgatt ccaccattat agagtaaatc
     1441 tacaggagct tttaatagag cagaaattaa tttggaacca gacaaagtgg aatcactaat
     1501 acctaaaact agtttcattt cttccgatat aggtatcgat ttttctgttt ttaaatatac
     1561 accaccccct cttgaaatga gagatggatt gtaattatcc cagccagacg cattagaaaa
     1621 gaaaaggcgt tttctttctt caaagctttt ttctggatca ggatttggat ctaggaatat
     1681 atgtttatga ttaaaagctg caactaactt aaaagctttt gattgaatca atccatttcc
     1741 aaaaacatct ccccccatat cacctatgcc aactaccgtt ataatatcct ttaaataatc
     1801 tatatctaga ttacggagtt gcctatcagc agtgactaat gctcctcttg cagttatacc
     1861 atattcttta tgagaatagc ctcttgatcc tcctgaagca aaagcatcac ctaaccaaaa
     1921 attatgtttt aatgatatag cgtttgccgt atcagaaagt tgtgcggttc ctttatcagc
     1981 agctactacc aaatatggat caaattcatc gtatgcgaaa ggcccgttca tatcgctaaa
     2041 aagaattttc tctgaacctt ttcggttatc ggtcaaagaa agaagagaat atacataggc
     2101 tttgtatgct tcaaccattg gtagctcaga agtaaattga tttttactta gaacaaagcc
     2161 tcctttggaa ccaacaggaa caatcacagt gttttttacc atttgagttt tcattaaaga
     2221 aagaatttct gttctaaaat ctgaaggccg atcggaaaaa cgaagtccgc ctcgtgcaac
     2281 agaacctcct ctcaaatgaa ttccttgaaa aagagaagaa taaacaaata tttctctaaa
     2341 tgggcgtgga agaggaagac tcactggtat actgctatca attttataag caacggctaa
     2401 tctttgtaat ccgctttctt taccaaaatt cgattcaaat ttagttccaa agaaatttgt
     2461 cctaagaatg gcaatcacag cggttcgaat agaatttaat acttcatctt cacgaatcgt
     2521 ttttgcttga tctgatagtt cttttaatct ggaatcagaa atcggttttc cttctctaaa
     2581 cttagaatag aaatatttcc atatttccgt agaaaattct ggatactgaa ccatgatttc
     2641 gttgaaatcg cttttattaa aagatggatt tgtttgaaga caatattccc gaatagattt
     2701 taaaaatagt aatgcagagg catctaaatt cgttattaaa atcaaagatg ataatgaatc
     2761 aacgggtaaa acaccttcca tcgtatatct aagagcattt tttaaacgat catagagatg
     2821 atctttattt tcgaataaat cgaagaaaaa ttcggtttta gaatatgttt ttcctgcaca
     2881 ttggaatcta tatactcttt gtgaaatagc cgagagtccg aaagcagtta gaaccgttac
     2941 ccattttgaa agattaaatt ctttttctaa agtaacagct tcgattagag tagcattaga
     3001 ttttgtttct gtaatattaa atattacttt tttgttttct aatcgttgaa ccaactctag
     3061 atcatataga gctttttcag gatcgtgagt tgctatataa tcatttctaa attcaacacc
     3121 attcggaaaa ggtaaatctt taaagcgatc ttcccatttt gttttaataa attcaaccca
     3181 aggtctaaat aaaaattcaa attgggaact aatttcgaat aagttttgtt ttgaaatatt
     3241 ctctgatcta attaaatgat agcattgaat tgaatttgta agtagaatat gataggtttt
     3301 aattttagcc ttttttaaaa ccgtttctac tatcttttca gtttcagcaa gttttgtttc
     3361 tgaccagttt ttttcaggaa tggttgtaag aataatagat aattctgaat ttaatattac
     3421 cccatttgat ctattctctg tttttagaga ttgttcaact atcactttaa agattgagat
     3481 ataagctgat tcgggaacca aaaataacac ttcgatagga aaggaattga atattcttct
     3541 aacacttctt cctaaacctg atgaatattc aactttttct ctatccaaaa aggctctaaa
     3601 tcttttatta aagaaaggaa tttcaattac tcctaacgtt tgtgcatgaa tagtaaagct
     3661 tccgatcaat aatactttta tatttttttg cacgaaagct atacaaaata aaggtgtatc
     3721 acctaaaaag ccttcatcct ttgtttctaa acattcaagg actagttcat ttgagtcatt
     3781 ttgtaatctc ttaaaccatt ctacatattg cattgtttct tcagcagatc caaatagttc
     3841 aagtttattg tctttgtcac ctataacacc agcttcccaa actagccctt tttttaagag
     3901 ccaatcaatt aacatccatt tatcagatat tgttactgct gagagactat ttagtttatc
     3961 aatgaaattt ggaaattgag tcgttagatt cgatatcttg ctataggact tagtgatttc
     4021 agctataatt ttttcaatca ggtctttatt tttagtttgt atttcaatat agtagaattc
     4081 ttttcctggt tctgggtgaa attgtaagct tctgttaatc ggaagatttg ttttatgaaa
     4141 tagggatcta atcttaccgg caaaaaatgg cattgattta catacaatta taatacaatg
     4201 taaattaatt ctaagaagtg gatcatctgc atttagatca t
//
LOCUS       DAOQOS010000241         4202 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_13821_length_4202_cov_6.292018, whole genome shotgun sequence.
ACCESSION   DAOQOS010000241 DAOQOS010000000
VERSION     DAOQOS010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4202)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4202)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4202
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13821_length_4202_cov_6.292018"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(301..1038)
                     /locus_tag="PLF66_25895"
     CDS             complement(301..1038)
                     /locus_tag="PLF66_25895"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HMZ67548.1"
                     /translation="MNRLILVEREKISESIYRLTDRKANHILKILKSNIGDSLKAGLW
                     NSSIGLARIESIDKEKREVFVSYQEEEKEIKKAGLSQLRVFSALQRPQTTKKIIQFCA
                     NCGILDLFFFPAEKSELSYLQSSIWTDESLEEEIILGLEQGGRVLSPNIQILKNKYRI
                     TDHLVNENRFILNFNHNYIDSFQNQISKTHPLHIVIGPESGFIEEDVKYFKNLSFKEV
                     SVSENILRSEIALMFFAAQLELTFNLR"
     gene            complement(1038..1451)
                     /locus_tag="PLF66_25900"
     CDS             complement(1038..1451)
                     /locus_tag="PLF66_25900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67549.1"
                     /translation="MKYIRPCPICNTELRFPLDMGTLIITCPNCSHSFRMDPDDPKTY
                     QTGTFDLSPSKKQTKFPIPLLSSLHSYLVSKKDIHFLIPLLLFLLLLLNLIKIFNTPV
                     AVEEEKPERQEYFEESPEAPPVPTPEKEKEPSIEI"
     gene            complement(1467..2330)
                     /locus_tag="PLF66_25905"
     CDS             complement(1467..2330)
                     /locus_tag="PLF66_25905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67550.1"
                     /translation="MKLITILFFLMSASLFAQKDGDTSKHSGDPDLFNGQSSAQVNAT
                     EEDLDKQITALNERLMKHTKLFGLRVESLPSKTVLFKGVEKGDDCEPAADNKQTDSAN
                     TCIKIEVFDFIQSEEGKTDLNIGPRSKYIILFYEGGGWGQDPAKEAPKKIKRIKSKIL
                     VNNFKETDLKISEVVDGDPHTPNGKSGENKHDEKITVFYQHDGLPVRGPKGEPPVEQK
                     DRKGYGLYKLADVENTRTNPTKNIFKQTYYIKHLIQFDKLFTKVYDMNDSRANKRYKE
                     SNQVLKSSLVY"
     gene            complement(3163..3945)
                     /locus_tag="PLF66_25910"
     CDS             complement(3163..3945)
                     /locus_tag="PLF66_25910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67551.1"
                     /translation="MKISKFFSILYLCVFIENCVSFADRLSISDDAIENKLIEWYYFD
                     FSQEQRQEENISLFGEKYLEFTTRKNDIDKGIFGVKVLLGSIDHITFQVAFCSLFFVR
                     EECFYERTKFYKNRQDPTFRIGAKIYPEFFKVDIQNSTYFFVFIQMIETGPIKFPEVL
                     VIRKGKKHLEYFISPLLSITSDRRAVGVFSQKQSFLNGDAGMKEFVLDSFQVVKDQIE
                     KNKISFKQIDFDLIENGYIVTREKKDGSIVKEEFLLSEDKSK"
     gene            complement(3960..>4202)
                     /locus_tag="PLF66_25915"
     CDS             complement(3960..>4202)
                     /locus_tag="PLF66_25915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016544430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HMZ67552.1"
                     /translation="PLPDPRERAAILENYAFHLTEDNRTQISNLLEGYSGRNLKDFCD
                     FVERKWAAAIIERGLQASPPPFEVYKEAAEVIQKNR"
BASE COUNT         1342 a          713 c          677 g         1470 t
ORIGIN      
        1 gattgagttt gggtgagccg cgcgttcaaa tgaggtttgc tcctattgtc attgaaaaat
       61 gacaatgggc ttgagcaaag cgaaagagaa ggaacggagg gcctgccaaa ctcaatgttt
      121 ctccctaagg gtcgaaacat aaggcaggct tttgatttta ttagtttatt agcattctct
      181 aatattttaa aactcatctt ttgtggctca atttattaat tttgtgtaag gtgagtgagt
      241 aactaattcg aaagaggttt tcaagaatca ctagaaatga tttagattgt atttcaaaaa
      301 ctatcttaaa ttaaacgtta gttctaactg tgcagcaaaa aacataagag caatttcact
      361 tctgagaata ttttcagaga cagagacttc tttaaagcta aggttcttaa aatatttaac
      421 atcctcttca ataaatcctg attcgggtcc tatgactata tgaagtggat gagttttcga
      481 gatttgattt tgaaaagaat ctatatagtt atgattgaaa tttaaaataa aacgattttc
      541 gttcactaag tggtccgtaa ttctatattt attttttaaa atctggatat tgggagaaag
      601 gacacgtcct ccttgctcta accctagaat aatttcttct tctaagcttt catctgtcca
      661 gatagaagat tgcaaataag atagttcaga tttctcggct ggaaaaaaaa ataaatcgag
      721 gattccgcag ttcgcacaaa actgtatgat tttttttgta gtctgtggtc tttgtaaggc
      781 agaaaaaacc ctaagctggg ataaacctgc ttttttaatt tctttctctt cttcttgata
      841 agaaacaaaa acttctcttt tctctttatc aatggactca atccgagcaa gaccgattga
      901 tgaattccag agtccagctt ttagagaatc cccaatattt gattttaaaa tttttagaat
      961 atgatttgct ttacgatcgg taagacggta aatagattcg gaaatttttt ccctttctac
     1021 taaaatcaat cggttcatta tatttcaata gaaggttctt tttccttttc tggagtcgga
     1081 acaggaggtg cttctggtga ttcttcaaaa tattcctgtc tctccggctt ctcttcttct
     1141 acagctacag gagtattaaa aattttaatc agattcaata agagcaataa aaatagtaaa
     1201 agaggaatta agaaatgaat atccttttta gatactaaat aactatgaag actagataga
     1261 agaggaatcg gaaatttcgt ttgcttcttg gaaggcgata gatcaaaggt gcctgtttga
     1321 taggtttttg gatcatctgg atccattcta aaagaatggg agcaattggg acaggtaatg
     1381 attaaggttc ccatatcaag agggaacctt aactctgtat tacagattgg acaaggacga
     1441 atatacttca cttcgcccta tctttcttag tatactaaag aactttttag aacttgatta
     1501 ctttctttat aacgcttatt agcgcgacta tcattcatat cataaacttt tgtaaacaat
     1561 ttatcaaatt ggatcaaatg tttgatgtag tatgtttgtt tgaaaatatt tttagttggg
     1621 tttgttctag tattttcaac atcagctaat ttgtatagac cataaccttt acgatctttt
     1681 tgctcaactg gaggctcgcc ttttggtcct cttactggta aaccatcatg ttgatagaaa
     1741 acagtgattt tttcatcatg tttgttttca ccagattttc catttggagt atgtggatcg
     1801 ccatctacaa cttcagaaat tttgagatca gtttctttga agttatttac taaaattttg
     1861 ctttttattc ttttaatttt ctttggagct tctttagctg ggtcttgtcc ccaaccacca
     1921 ccttcgtaga aaaggatgat atatttgctt cttggtccga tgtttaaatc agttttacct
     1981 tcttctgatt gaatgaaatc aaaaacttca attttgatac aagtgttagc tgaatcagtt
     2041 tgtttgttat cagctgctgg ctcgcaatca tcaccttttt caacgccttt gaagagaaca
     2101 gttttgcttg gtaaagattc aactctcaaa ccaaatagct ttgtatgttt catcaatctc
     2161 tcattcaaag cagtgatttg cttgtcgagg tcttcttctg ttgcatttac ctgagcagag
     2221 ctttgtccgt tgaataaatc aggatcgccg gagtgcttgc tcgtatcgcc gtccttttgt
     2281 gcaaaaaggc tggcactcat taaaaaaaat agaattgtaa tcaatttcat aaagtcattc
     2341 ttccttaaat cttaatacga tttctttttg gataatagta attactgtgg aatttatgta
     2401 aagtattatc ctatctaatt atcggtcttt tagaaactta atttaataaa gttaaaattt
     2461 ttacttatga tttaaagaaa ttcctgcatg aatgaactag aacttgtttt aaaaactcgc
     2521 atactatttt tactgaaaaa ccaagcgact aaaacctatt tagtagactt tccaagctta
     2581 attctagagt aaaaacctca gccgaatcca aacctccaag caactggaag aggatctgaa
     2641 atagaaaaaa gtagaactga gaatcactat ttatagcatt tcaaaaagta tttcctttag
     2701 tcctttgtaa tgggtttgta tagtggttga tagagtgaaa ttcaattttg aatctatttg
     2761 aaactaaata aatctaaatc ctaaacctaa aaggctggtt ctaaagaata tagatttagt
     2821 tatagtaggt taaatatcag acggctcctc taataaaaat agggaagcca gactgatgat
     2881 tcgattcaaa ttaaggcgat ttagcagaaa ttgttccacc gcttgtgatt gccacaaaga
     2941 ttccacttct ttaagcaatg gaaatccaag gagtgaaagt cggtaaggcc tgcttatcca
     3001 gtccatacca cggtaggagt aacgaaagca ttgctacttg atttccctcc agtgactaaa
     3061 ctacacatct gcctctttct tttacacact tgcgcgtaag atgaacaaat aatttaaatt
     3121 ttaaaaaatt agaaagataa aacaaaagat atgaacttat ttctattttg atttatcttc
     3181 agataaaaga aattcttctt taacaataga tccatctttt ttttctcggg taacaatata
     3241 gccattctca atcagatcga aatcgatttg cttaaacgag attttattct tttcaatctg
     3301 atccttaact acttgaaaac tatctaaaac aaattccttc attcctgcgt cgccatttaa
     3361 gaaactttgc ttttgagaga acacacctac tgctcttcga tcacttgtta tacttaataa
     3421 aggacttata aaatattcta aatgcttttt cccttttcga atcactaaaa cctcagggaa
     3481 cttaatcgga cctgtttcta tcatttgaat aaaaacaaaa aaatatgtag aattttgaat
     3541 atcaacttta aaaaattcag gataaatttt ggcaccaatt ctaaaagttg gatcttgtct
     3601 atttttgtag aatttcgtcc tctcataaaa acattcctct ctaacgaaaa aaagcgaaca
     3661 aaaagctact tgaaacgtta tatggtctat actacccaat aaaactttta cgccaaaaat
     3721 gcctttatct atatcatttt ttcgagttgt aaattctaaa tatttttctc cgaacaaaga
     3781 aatattttct tcttgtcttt gctcttgtga aaaatcgaaa taataccact caattaattt
     3841 attttctatt gcatcgtctg atatagaaag tctatcagcg aacgatacac aattttctat
     3901 aaatacacaa aggtatagaa tagaaaaaaa tttagatatc ttcattagca tttttactat
     3961 tagcggttct tttgaattac ttctgccgct tctttataaa cttcaaaagg aggaggagaa
     4021 gcctgtagtc ctctttcgat aattgcagcg gcccatttgc gttctacgaa gtcacaaaag
     4081 tccttcagat ttctaccaga ataaccttct agtaaatttg atatttgtgt gcggttgtcc
     4141 tctgtaagat gaaacgcata gttttccaaa attgctgctc tctctcttgg atcgggtaat
     4201 gg
//
LOCUS       DAOQOS010000242         4146 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_14118_length_4146_cov_6.047666, whole genome shotgun sequence.
ACCESSION   DAOQOS010000242 DAOQOS010000000
VERSION     DAOQOS010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4146
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14118_length_4146_cov_6.047666"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            48..482
                     /locus_tag="PLF66_25920"
     CDS             48..482
                     /locus_tag="PLF66_25920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67553.1"
                     /translation="MAKRKSKFNLLIGGLGLLGAGAVYYKTGTGRIVKNSYKNVIHVK
                     PESSVEAQPIPAGSLVDKIDGISTPFRPNQVYKVPNRMDVEITETGDVKPVNSNFIDG
                     LAFEVGILDYGWRDKTSAWYQKLLKSKDTGWDELFETSAYKQ"
     gene            497..880
                     /locus_tag="PLF66_25925"
     CDS             497..880
                     /locus_tag="PLF66_25925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67554.1"
                     /translation="MIQKIINWVSIILVLVFAFYFTLYSFIDGKSNLTFDSAISDTDY
                     SKGYSDLAFLSVKEGQSKEQVIKKIGEGFECIDREKRDENIFPSNYILFCKYSKSDRY
                     AYRTRMIVYDKDNKVIAKLTGVIPD"
     gene            880..1311
                     /locus_tag="PLF66_25930"
     CDS             880..1311
                     /locus_tag="PLF66_25930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67555.1"
                     /translation="MKATTRPLTSYQNTVAHIIRIAREYTQDVSGFDNFFQLYNYVRD
                     LPYKSDPVNQETVSRFLYTKNYDFPIRDCDDKTVPLVSYAILKKIPVRVVVCGEIETP
                     HHVYPEIFFGDSWLPADATYDDRCSFGKYLYNEKFRKIFPV"
     gene            1484..2545
                     /locus_tag="PLF66_25935"
     CDS             1484..2545
                     /locus_tag="PLF66_25935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67556.1"
                     /translation="MQIVSLEGNFIPYPDSLNGDSDTLEGDLHVDKAIVEIPLNGKIY
                     TYELELDGGFKLGKVKAPRMPRMPRMPRVRVNTRGLSRSVSRAGQGLSKAATSAGRAY
                     GNFVNNNIKQIGQIAQGAGSLLSNVAQGAGSLLSNFGNSDSGEDQPDETEQTEESFQD
                     DEGIENLPNEENEELNGLLSTIGSAAGSFLMPGVGTAAGGMIGNMLEQGQKKPVKKAK
                     QIKRRKITQKDTARLSNIRARQTSINPQNQNVNRASQLNQNISYSQPQQPQRQIALKS
                     YPDGTFSLAPKVRKAEEITDANPTGNADNPPTSDPIAPTTDPNNPPAKKKDDNTMLII
                     GGVAVAGFLMFSMNKKGRK"
     gene            2549..2734
                     /locus_tag="PLF66_25940"
     CDS             2549..2734
                     /locus_tag="PLF66_25940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67557.1"
                     /translation="MKKRLKLLKYRRKPKKNASNAVKEAWLSHKHDVDKENAKRKSDY
                     EKAKRKQAELDKKLYRG"
     gene            2738..3322
                     /locus_tag="PLF66_25945"
     CDS             2738..3322
                     /locus_tag="PLF66_25945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67558.1"
                     /translation="MSGIMKEGMSDAKNAGLILAGLAISKFVSVELLKDFDKEKKFKP
                     LLIPIAGYFAAREVPIKWARQGLKEGFLVSLYGGGLDMLNLKKEKDYVGLAGDRDESD
                     VRTFVFNGMSEVRDFADALVNKRQANAQLPPPVNNNKEDFIVHGVIVESSETDRVIQP
                     ELAGRASDFDNGDYTGLMGEYRYEGDPILNGDFE"
     gene            3348..3962
                     /locus_tag="PLF66_25950"
     CDS             3348..3962
                     /locus_tag="PLF66_25950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67559.1"
                     /translation="MKLKMDNGTFYEDIELISDVTLKGGLTKIPLFKVNSTVVATKNN
                     TTGQVNPFTSDMGGVIKSLKFTHFFPGYVLKTYSAGTLAKLSGYSQLRSGQVFLKRGS
                     DIIMQRSMEEFLPPPPEVLLPEDVNAPAAEPSFKGFRPSPIAFQPVYDVKKLVKVWSK
                     GLRYQQNENLELWIDLPEGISTDPAVTDSVLRAKFDAAALSSKA"
     gene            3965..>4146
                     /locus_tag="PLF66_25955"
     CDS             3965..>4146
                     /locus_tag="PLF66_25955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67560.1"
                     /translation="MAEKVTYDYFLSGVPERKIPQGSEKEAFSIINSLNEDYIITGYQ
                     VKYNAALKNSRLKMKDG"
BASE COUNT         1496 a          629 c          817 g         1204 t
ORIGIN      
        1 ttgacaaaaa atctcacaaa tagtaaaata aaaaaaggag aataagaatg gcaaaaagaa
       61 aatctaaatt taatctatta atcggaggct tagggttatt aggcgctggg gctgtgtatt
      121 ataaaacggg aacggggaga atcgtaaaaa acagttacaa gaatgttatt cacgtaaaac
      181 cagaaagctc tgtagaagct caacctattc cggcaggatc attagtagat aagatagacg
      241 gaatttctac accatttcga ccaaatcaag tttataaagt tcctaacaga atggatgtag
      301 aaataacaga aacgggagat gtaaaacctg ttaattcaaa ttttatagat ggtcttgctt
      361 ttgaagttgg tatattagat tatggttgga gagacaagac aagcgcatgg taccaaaaat
      421 tattaaaaag taaagatact ggttgggatg agctatttga aacatctgca tataaacagt
      481 aaaggagatt tttaaaatga ttcaaaagat tataaattgg gtaagtatta ttttggtttt
      541 agttttcgct ttttatttta ctctctattc ttttatagac ggtaaaagta atttgacttt
      601 tgactctgct atatctgaca cagattattc taaaggctat tctgatttag cctttctttc
      661 tgtaaaggaa gggcaatcga aagagcaagt tattaaaaaa atcggtgaag gttttgaatg
      721 tatagacaga gaaaaaagag atgaaaatat tttcccttct aattatattc ttttttgtaa
      781 gtattcaaaa agtgatcgtt acgcatacag aacaagaatg atagtttatg ataaggataa
      841 taaagtcatt gcaaagctaa cgggagttat acccgattaa tgaaagcgac tacaagacct
      901 ttaaccagtt atcaaaacac agtagcgcat attatccgta tcgctagaga gtatacgcaa
      961 gatgtttctg gatttgataa tttttttcaa ttgtataatt atgtaaggga ccttccttat
     1021 aaatcagatc cagtaaatca agaaactgtt tcaaggtttt tgtatacaaa gaattatgat
     1081 tttcctatta gggactgtga tgataaaaca gtccctttag tttcttatgc cattttaaaa
     1141 aaaattcctg ttcgagttgt tgtttgtggc gaaatagaaa cacctcatca cgtttatcct
     1201 gaaatatttt tcggtgactc atggctaccg gctgacgcta cttatgatga tagatgttct
     1261 ttcggaaaat atttatacaa cgaaaaattt agaaaaatct ttcccgtcta ggtgctacat
     1321 agcaccttta aaaaaaatta aataatcttt taaataaatc acaaatacgc gacaaataga
     1381 gagtatccga aactttaaaa acttgctcta atttttaatt cctttatgat ttgcaattgt
     1441 gaaagaatta ataacccttg aaggcgatct agaaaattat gatatgcaaa tagtttctct
     1501 agaaggaaat tttattcctt atcccgattc tttaaacgga gactcggaca cattagaagg
     1561 ggatttgcat gtagataagg ctattgtcga gattcctctg aatggaaaaa tatacaccta
     1621 tgaattagag ttagatggag gctttaaact tggcaaggta aaagctccac gtatgccgag
     1681 aatgccaaga atgccgagag taagagtaaa cacaagagga ctttctagaa gtgtttcaag
     1741 agcaggacaa ggactttcta aagcagcaac ttctgccggt cgggcttatg gaaattttgt
     1801 aaataataat ataaaacaaa taggtcaaat tgcacaaggt gcaggaagtc tgctaagtaa
     1861 tgttgcacaa ggtgcaggaa gtttattaag taattttgga aatagtgata gtggtgaaga
     1921 ccaacctgat gaaacggaac aaacagagga aagttttcaa gacgatgaag gaatagagaa
     1981 tttaccaaat gaagaaaatg aagagttaaa cggtttatta tctactattg gatcggctgc
     2041 cggttctttt cttatgcctg gagttggaac ggctgctggt ggcatgatcg gaaatatgct
     2101 agagcaagga caaaaaaaac cagttaaaaa agctaagcaa ataaaaagaa gaaaaataac
     2161 gcaaaaagat acagcgagac tatcaaatat tagagcaagg caaacaagca ttaatccaca
     2221 aaatcaaaat gttaatcgtg caagtcaatt aaatcaaaat atatcttatt cacagccaca
     2281 acaaccacaa agacaaattg cattaaaatc ttatccagat ggaacttttt ctttagctcc
     2341 taaagtaaga aaagcggaag aaataacgga tgcaaaccct acgggtaatg cagataatcc
     2401 accaactagc gatccaatag cgcctacaac agatccaaat aatccaccgg ctaaaaagaa
     2461 agatgataat acaatgttaa tcatcggtgg cgtagctgta gccggttttc ttatgttttc
     2521 aatgaataaa aaagggagaa agtaataaat gaagaaacgt ttaaagcttt tgaagtatag
     2581 aaggaaacct aaaaaaaatg cttcaaatgc agtaaaagaa gcctggttat ctcataagca
     2641 tgatgtagat aaagagaatg caaagcgtaa aagcgattac gaaaaagcca aacgcaagca
     2701 agcagaactt gataaaaaac tttatagagg ttaagtcatg agtggaatta tgaaagaggg
     2761 aatgtcagac gcaaaaaatg cgggattaat tcttgcaggc ttagcaattt ctaagtttgt
     2821 ttctgttgaa cttttaaagg acttcgataa agaaaaaaaa ttcaagcctt tgttgattcc
     2881 gattgctgga tatttcgcag ctcgtgaagt tcctattaaa tgggcaaggc agggattaaa
     2941 agaaggtttt cttgtctctc tttatggtgg tgggctagat atgcttaacc tcaaaaaaga
     3001 aaaagattac gtaggcttag ccggtgacag agatgaaagc gatgttagga cttttgtttt
     3061 taatggaatg tcagaagtta gagactttgc ggatgcactt gtaaacaaaa gacaggctaa
     3121 tgcacaatta cccccacccg taaacaataa caaagaagac ttcattgttc atggtgttat
     3181 tgttgaatca tcggaaactg atagagttat tcagcctgaa ttagccggta gagctagcga
     3241 ttttgataac ggagactata ccggtttaat gggtgagtat cgttatgaag gcgatccgat
     3301 tttaaacggt gattttgagt aagtaataat tttaggagaa tatttttatg aaattaaaaa
     3361 tggataacgg cacattttac gaagacatcg aattgatttc agatgtaaca ctgaaagggg
     3421 gattaacaaa aattcctcta ttcaaagtca attcgactgt agttgcaaca aagaacaata
     3481 ccacaggaca ggtaaaccct tttacctctg atatgggcgg tgttattaag tccttgaagt
     3541 ttacgcattt tttccctggt tatgttttaa aaacatattc tgcaggaaca cttgcaaaac
     3601 tttctggtta ctcacaatta cgctcaggac aagtttttct aaaacgtggt tctgatatta
     3661 ttatgcaaag gtcaatggaa gaatttcttc cacctcctcc agaagttctt ttaccggaag
     3721 atgtaaacgc acctgcagcg gaaccaagtt ttaaaggctt tcgtccttcg cctatcgcat
     3781 ttcaaccggt ctatgatgta aagaaacttg taaaagtttg gtcgaaaggc ttacgttacc
     3841 aacaaaacga aaacttagaa ttatggattg atttaccaga aggaatttcc acagatccgg
     3901 ctgtaactga ttctgttctt cgtgctaagt ttgatgcggc tgcattatct tctaaggctt
     3961 aacaatggct gaaaaggtta cttatgatta tttcctttct ggagttccag aaaggaaaat
     4021 tccgcaagga tctgagaaag aagctttctc tattattaat tctttgaatg aggattatat
     4081 aataacaggg tatcaagtta aatacaatgc tgctctaaaa aattctcgtt taaaaatgaa
     4141 agatgg
//
LOCUS       DAOQOS010000243         4045 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_14722_length_4045_cov_4.863409, whole genome shotgun sequence.
ACCESSION   DAOQOS010000243 DAOQOS010000000
VERSION     DAOQOS010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 4045)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4045)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4045
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14722_length_4045_cov_4.863409"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            262..1341
                     /locus_tag="PLF66_25960"
     CDS             262..1341
                     /locus_tag="PLF66_25960"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HMZ67561.1"
                     /translation="MHSGGLYNFEIFKSYYPFGMVTPGRSYSAGSGYRFGFNGKESDD
                     QLYGENNGYDLGLRIYNPRLGRFLSLDSKSREYPWQTPYAYFFNNPVAVIDVLGAGAP
                     DGEKELSEGDGATPTQYTIKPKDNFWILENKLGLPHGNLQKWNPTLDPNNLQIGTVIN
                     TSDPYYYDIENAVEFSVLPFDGGDFTPRSFIPAQYDGMPTNLWMIYSEFVKGTGPVNS
                     IFIENPLITGQLASSNGVNEFRNFMYSKFDGSLSKMQEYASNHNRALVTDFDPYGSFY
                     PWDEIGNGVMQFIGSCSVDAFLSYDNKTIIYVVTDSKSLYSLILHSTNRLDYERTKQG
                     DAYSNTYQKYIWFETFNSQYGPENK"
     gene            1460..1726
                     /locus_tag="PLF66_25965"
     CDS             1460..1726
                     /locus_tag="PLF66_25965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67562.1"
                     /translation="MRYKGENIFPNEFVNKVGYNSDWIVANTSSFQENITSSKYYIIN
                     KQNYAAQLGETKNLGVMGPFDSINYEETLSELHLQNECKFIISF"
     gene            complement(2388..3323)
                     /locus_tag="PLF66_25970"
                     /pseudo
     CDS             complement(2388..3323)
                     /locus_tag="PLF66_25970"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
     gene            3544..>4045
                     /locus_tag="PLF66_25975"
     CDS             3544..>4045
                     /locus_tag="PLF66_25975"
                     /inference="COORDINATES: protein motif:HMM:NF013698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HMZ67563.1"
                     /translation="MKDYSIFIGIDVSKSTIDVACLQKGTWTLKLKLENNKSGVQTLI
                     RNLKKQPGFEPQDTLVCMENTGMYSYKTALLLIDLGIKNIWIESALQILRSQGVVRGK
                     SDSIDAVRIAEYALRFQSRATIFEFSNETFYLKTLLNLRTLILSSLHRFKVNLAEFKE
                     MEGLIDL"
BASE COUNT         1356 a          687 c          686 g         1316 t
ORIGIN      
        1 tagatgtttt ttcataaaaa tcagacgcta taaattccgg tttattcaat atacaagata
       61 atgagaaatg cgcttctaga acttggcagg tgatagatag tatgggtggg tatcgcattc
      121 tcaaacaaat aggctaccct ttttttccat tgccaagcaa atttgcggat gcagattcgg
      181 caaatctcga taattgaatt ttatattaat gaacaattaa ttgaatcttg aactgctaat
      241 taaatcttgg ttaacttaat catgcacagt ggtggtttat acaattttga gatatttaaa
      301 agttactatc cgttcgggat ggtaacgccg ggacgctcct attccgctgg aagtggctat
      361 aggtttgggt ttaacggcaa agagagtgac gaccaattgt atggagagaa taatggatat
      421 gatttgggat taagaatata taacccacgc ctaggtagat ttctatcttt agattcaaaa
      481 tcacgggaat acccttggca gacaccctat gcatacttct ttaataatcc tgtggcagta
      541 attgatgtcc ttggtgcagg tgctcccgat ggcgagaagg aattaagcga aggagatggc
      601 gctacaccta cgcagtatac aataaaacca aaagacaatt tttggatact agagaacaaa
      661 cttggtttac ctcatggtaa tctacaaaag tggaatccaa cacttgatcc taataattta
      721 caaatcggca cagtaataaa tacttcggat ccatactatt atgacatcga aaacgcagta
      781 gagttttctg tattaccatt tgatggtgga gacttcacac ctagatcttt tatacctgca
      841 caatatgatg gaatgcccac caatttatgg atgatatatt ctgaatttgt taaaggaaca
      901 gggcctgtaa acagcatttt tattgagaat ccactgatta ctggtcaact tgctagtagt
      961 aatggcgtaa atgaatttcg aaactttatg tattccaaat tcgatggaag tctttcaaag
     1021 atgcaggaat acgcctcaaa tcataatcga gctttagtca cggattttga tccgtatggt
     1081 agtttttatc cttgggatga gattggcaat ggtgtaatgc aatttatagg tagttgtagt
     1141 gttgatgcat ttttatccta tgacaacaaa acaataatat acgtagtaac agattctaaa
     1201 agcttatact ctttgattct gcattcaaca aaccgattag attatgaaag aacgaagcag
     1261 ggtgatgctt attcaaatac ataccaaaag tacatttggt ttgaaacgtt caattcccaa
     1321 tatggcccag aaaacaagta aaattcttag atttcttatt gcttttctag tagtaagcat
     1381 actgataggt tgcaataagt caataaatat tgatgggaat ttctatgttc aaaaggattc
     1441 actaagacag aagtatattt tgagatataa aggagaaaat atatttccaa atgaatttgt
     1501 taataaagtt ggatataata gtgactggat agttgcaaac acttctagct ttcaagaaaa
     1561 cattacaagc tccaaatatt atatcattaa caaacaaaat tatgctgcac aacttggtga
     1621 aacaaaaaat cttggtgtta tgggtccctt cgattcgatt aactatgaag aaactttgag
     1681 tgagttgcac ttgcagaacg aatgtaaatt tataattagt ttttagttat tcaagaatgg
     1741 cgctaaatgc taaccaaaat taaattcgag taggaatttt aaattatatt aatgttattt
     1801 gataattatt tatcaactag ctttattaat tttaagtgaa gattcaactt acctagttgc
     1861 atacaaaata aaataaatat ttctgatata caatttaatc atgatcttgt tgttaaagct
     1921 cttcgccgaa aattccttct cacaattcgt aagaggtggt caagttgtct attctaaacg
     1981 aatagattta tatactataa atggcgaaat tattactggc gcaaacattg aaataaaaaa
     2041 cgcaaagctc gaaagcagta aatttatttg caaagcccaa cttaatatta ttgtaacaag
     2101 aagaaaaact gcacaatgta taattatgtt tattgtctgg ctgaacttgc ggttagatat
     2161 ataataggta tataaaaagt aaattggcaa ataaatcatg catttaacgg gaaatattgt
     2221 ttttaaatta tctaaaataa aactgcgtaa tgtatcaagt ccaggagatt cggtgaaata
     2281 ttttataaca aatgcattta gaagaatttg cattattgtt tctaaaagaa atattaacaa
     2341 aatttgccaa acaagtttaa aacttctcat aaatcgacat atttatttta tttattttga
     2401 gcagaatttg cttctaatcg atggtactct gcaattttct tgcctaaaaa aattctatga
     2461 tcgagattca taaaatcaaa atcttcatcc gtaccatatt ttttatcaaa ttcttccgcg
     2521 gcgtatttat ttgcttccgg tgtaaattgt ttgaataaat cattctcaaa taaatagttg
     2581 atattagcca ccatttgatc ccatgcttca atgctatttg attttagata ttctgtttta
     2641 tattcaacaa taaaattgtc cagtaagact tgctctattc gcaccatttc ctcatctaaa
     2701 tctgctgttg aataatcata aagtgaccat cctgcttcgt tttttatttt accatctttc
     2761 tccacaagaa aactcccaaa attattcatg ttttgcgtaa acaaataaac atttgccgca
     2821 attaggaaat ctttagcatc gcttgaaata aaccagaaat taaatttttc agctcgtccg
     2881 accataaaag gtcctgttac atctgctgct gctccatacc agtaaacatc ttttttttgc
     2941 cctatttcgt ccgcccaaga ataccaatta tgcctgtctt caactgactt ataaacatct
     3001 ggattagaat tactggtatt atatacagct gctgcattaa atgattgcca gtctacttgc
     3061 ccttcgataa cgtaccattc tgaggctttc aattctggtc ttgaatagta gaattcgaac
     3121 caggtacaat acacagcttc caatccgtcg agatcgattg cttgaatagg ggtattactt
     3181 gcgaattgga aaggtgtcaa tgcaggataa gaacttgtta atggatccac gctcaaaaac
     3241 ttcccaagtc tcggattata gatcctaaac ccataatcat aaatattgcc actaccatag
     3301 gtttccgggt cactctcttt gccgttaaac ccctttaggt ttgtatttta taaataaaga
     3361 acaatcagta attttagtaa ataaggaggt gaactatgtt aaaccccggt tttacggacc
     3421 acggtttgat tcagacctcc ttattttact tgagataatg tcatcaaata gaaatttagg
     3481 ctaattacct attatcaagg gttcgatgta gttatctcca ttttttaacg ctaaatcaaa
     3541 tatatgaaag attattcaat tttcattgga attgacgtat ccaaatcaac gattgacgtc
     3601 gcctgccttc aaaaaggcac ctggacttta aaattgaagc tagaaaacaa caaatccggg
     3661 gttcaaaccc taattcggaa tctaaaaaaa cagccaggtt ttgaacctca agacacattg
     3721 gtctgcatgg agaatacagg catgtattca tacaaaactg ctttgttgct tattgactta
     3781 ggtatcaaaa atatctggat cgaaagtgcg ctgcagattc ttcggtcaca aggcgttgtt
     3841 cgtggaaaat cggatagcat tgatgctgtc cgaattgcag aatatgctct tagatttcaa
     3901 tcacgggcca ccatctttga attcagcaat gaaacgtttt atcttaagac attgctaaac
     3961 ctcagaacgc ttattctgtc ttcattgcat cgatttaaag tcaatctagc ggagttcaaa
     4021 gaaatggaag gactaattga tctca
//
LOCUS       DAOQOS010000244         3989 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_15096_length_3989_cov_5.461108, whole genome shotgun sequence.
ACCESSION   DAOQOS010000244 DAOQOS010000000
VERSION     DAOQOS010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3989)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3989)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3989
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15096_length_3989_cov_5.461108"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            119..736
                     /gene="coaE"
                     /locus_tag="PLF66_25980"
     CDS             119..736
                     /gene="coaE"
                     /locus_tag="PLF66_25980"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HMZ67564.1"
                     /translation="MKEWNKRFVLGITGSMGGGKSTVTKIFEKLGAFRISSDEIARSF
                     TEKDSPIKEELIQNLGKEILDENGDLDRKRIAGIVFSDKNAIAKLNGLIHPLVRKKTL
                     ELIDSVIDGRLVAWEAPLLFEAKGDAICDATLTVYAEYNDLWNRVKDRDGISEEEFRQ
                     RLANQMDIKKKLEASDFKIINNKDVRHLEDECANIYNSILNKKLS"
     gene            755..1528
                     /locus_tag="PLF66_25985"
     CDS             755..1528
                     /locus_tag="PLF66_25985"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HMZ67565.1"
                     /translation="MKERTFYTINLDLRRIVVISVVFIGLLIYFFMLGNAMGKKSVAA
                     KDPSKDVADTSKAEMKASEKSLLKPSPEENMVPAPPEEKTEPKDSEIVDLKPSTDTTK
                     PIPVPAPSPVQEVKEELTKKPVAINTPQPNAKTVSPVAVNPQPLNPNLPAKKPIYPYP
                     AKKVATLPAKTPIVLEEKNIYTIQLGAFSSQEQANKFKDNVITKNKFPNKFAPYVLKQ
                     RDFFVVQVGKSTAKEDLEKIVQNLDPATQGSAMIVKNKK"
     gene            1531..2130
                     /locus_tag="PLF66_25990"
     CDS             1531..2130
                     /locus_tag="PLF66_25990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67566.1"
                     /translation="MIKEFFISFFFISFISLYAVEPPNEPTPPTVDLADKTSPVKKDS
                     KKDKPKKTQAVTVKLCDGRQVSGNIEYEKEEIFFQHLKEGIKYDKKMQITEIKQIKIL
                     SWDGKKGKKVKDGITYQFNPSKVQITSLSSESFIIKGLADTEFQNLNISNQNGIAKLF
                     SFWVDLQYESGNWYSKLPAINGTEREDCHSDVIRIIQFN"
     gene            2133..3230
                     /locus_tag="PLF66_25995"
     CDS             2133..3230
                     /locus_tag="PLF66_25995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67567.1"
                     /translation="MPINLPNHLNLFWIKLEHLLNALDDMQNRLNPKRISEYIEKLKP
                     ISMEIKTYCDKLGTIDSFDSNSVEFMLLESAFQLILSYTKMESANQNPKEILKMFQAL
                     RPITRAKEILYPLANQFEEVSKYFLTKNRREDPIFKNKLDLVKETQDKTKIGIFHFEN
                     DRSQRGGYSLYVPEYYSEEKSWPLVVTLHGGSGHGLDLFWNWVREARSYGFIIASPTS
                     RGRTWSIYNPMLDVVNLNQMLSSISGKWRINTDQILLTGISDGGTYSMLLSITHQSPF
                     THYAPIAAAVHVLINRLGEISAPVKGKKIYQVHGREDWMFPVEKEREVANQLEKAGAN
                     IVYREIADLSHNYPQDENENMLRWFYPKAFE"
     gene            3223..3969
                     /locus_tag="PLF66_26000"
     CDS             3223..3969
                     /locus_tag="PLF66_26000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67568.1"
                     /translation="MSKNLYRLILFLLLVFYILNCNRRVNLIPNHLYQKENYKENLFS
                     RNEKINLILKIEAEEDFELEDVNFYLANGFPEEYSEDAYELGYLFHIPFIYKVKHIAS
                     IDSIYTYTEKIEIKNGNVYEFVLEPNTYYALLERKGGQEFSFLDFKRITVYFGYIKKG
                     SLYNKVKEDVHCQEIQDSSSHKAYEISCPVISIEENKITNIQIKIGKKKWNSFFSIFL
                     KFIPGILFLGPFNNLPQPYFHRDIRIEITK"
BASE COUNT         1478 a          598 c          716 g         1197 t
ORIGIN      
        1 gttcgatccg ttgatccttt ttggcggcaa tctttgattc cttttcttaa ctaaaggaga
       61 ttcaactgag tggggtgagt tagaaatcag aattgtccga tatggataag agggatttat
      121 gaaagagtgg aataaaagat ttgttttggg aattactgga agtatgggtg gtgggaaatc
      181 tactgtaact aaaatttttg aaaaattggg tgcgtttaga atctcatccg atgaaattgc
      241 acgaagcttc actgaaaagg attctccaat taaagaagaa ctgatccaaa atctaggaaa
      301 ggaaattcta gatgagaatg gagatctgga tagaaaaagg attgcgggaa ttgtgttttc
      361 tgataaaaac gccatcgcaa agttgaatgg actgattcat cccttggttc ggaagaaaac
      421 cttagaattg atcgattctg tgatagatgg tcgcttggtt gcatgggagg cgcctctttt
      481 gtttgaagca aagggagatg cgatctgtga tgctacctta accgtttatg ctgaatacaa
      541 tgatctttgg aatcgagtaa aagatagaga tggtatttcg gaagaagagt ttcgacagag
      601 gctcgcaaat cagatggata tcaagaaaaa gttagaagca tccgacttta agattataaa
      661 taataaagat gtaagacatc tagaagatga atgcgccaat atttataatt caatcctgaa
      721 taaaaaatta tcctaaaatg gatagaggaa acatatgaaa gagagaacat tttatacaat
      781 caatttagat ttgcggagaa tcgttgttat atccgttgtc tttattggtt tactgatata
      841 tttttttatg ttaggcaatg ctatgggcaa aaagagtgta gctgccaaag atccatctaa
      901 ggatgtagca gatacaagca aggctgagat gaaagcctct gaaaaatctc tcttaaaacc
      961 ttcccctgaa gaaaacatgg ttcctgctcc tccagaggaa aaaacagaac caaaagattc
     1021 tgagattgta gatttaaaac cgtcaacgga tacaactaag ccaattcctg ttcctgctcc
     1081 atctcctgtc caagaagtaa aagaagagct gacaaaaaaa ccagtagcaa ttaatactcc
     1141 acagccaaat gcaaagactg tatcgcctgt cgctgtaaat cctcagccac tgaatccaaa
     1201 cttaccggct aagaaaccaa tttatccgta tcctgctaaa aaagttgcga ctcttcctgc
     1261 taagacacct atcgttttgg aagagaagaa tatttataca attcaacttg gagcctttag
     1321 ctcgcaagaa caagccaata aatttaaaga caatgtgatt acaaaaaata aatttccaaa
     1381 taaatttgct ccctatgtat taaagcaaag agatttcttt gtggtgcaag ttggtaagtc
     1441 aactgcgaag gaagatctag aaaagattgt gcaaaattta gatccagcta cacaaggatc
     1501 tgcgatgatt gtaaaaaata aaaaataggg atgattaaag aattttttat ttcatttttc
     1561 tttatttcat tcatttcttt atatgcggtg gaacctccaa atgagccaac tccaccgact
     1621 gtagatttag ctgataaaac gagtccagtt aaaaaagatt ccaaaaaaga caaaccaaaa
     1681 aagacacaag cagtaacagt taaactatgc gatgggcgac aagtatccgg aaacattgag
     1741 tatgaaaagg aagaaatttt ttttcagcat ttaaaagaag ggattaagta tgataaaaag
     1801 atgcaaatta ctgaaattaa acaaattaag attctttctt gggatggtaa aaaaggaaaa
     1861 aaagtaaagg atgggataac ttatcaattc aatccatcca aagttcaaat tacaagtctt
     1921 agttcagaaa gttttattat aaaaggtttg gcagacacag agtttcaaaa tttgaacatt
     1981 tccaatcaaa atggtattgc aaaacttttt tctttttggg tagatctaca atatgaaagt
     2041 ggaaattggt attccaaact tccagcaatc aatggaacag aaagagaaga ttgtcattcg
     2101 gatgtaattc gaattataca attcaattaa aaatgcctat aaacttacca aatcatttaa
     2161 atcttttttg gataaagcta gaacatctat taaatgccct agatgatatg caaaatagac
     2221 tcaatccaaa aagaatttct gaatatattg aaaaactaaa accaatctca atggaaataa
     2281 aaacttattg tgataagctt ggaactatcg attcgtttga ttcaaattca gtcgaattta
     2341 tgcttctaga atctgccttt caattgattc tatcctatac aaaaatggaa tctgcaaatc
     2401 aaaatccaaa agaaattttg aaaatgtttc aagcacttcg cccaattaca agagcaaagg
     2461 aaattttata tccattagca aaccagtttg aagaagtctc aaaatacttt ttaacaaaaa
     2521 atagaagaga agacccaatt tttaaaaata aattagattt agtaaaagaa acacaagaca
     2581 agactaaaat cggaattttt cattttgaaa atgatagatc gcaaagggga ggctactctt
     2641 tgtatgttcc agaatattat tcggaggaga aatcttggcc tttagttgtg actcttcatg
     2701 gtggatcagg acatggatta gacctttttt ggaattgggt tagagaagct cgcagttatg
     2761 gattcataat tgcttccccg acgtcacgcg gtaggacatg gtcaatatat aatcctatgt
     2821 tggatgttgt gaatttaaat caaatgttat cttcaatctc tggaaaatgg agaataaata
     2881 cagatcaaat tcttcttaca ggtatttcag atggaggaac atactccatg cttttatcta
     2941 tcactcatca gtctccattt actcattatg cgccaatcgc agctgccgtt catgttttaa
     3001 taaaccgtct cggtgaaata tctgcaccag taaaaggaaa aaaaatctat caagttcacg
     3061 gaagggaaga ttggatgttt ccagttgaaa aagaaagaga agttgcgaat caattagaga
     3121 aagcgggagc taatatcgta tatcgtgaaa ttgcagatct ttctcacaac tatccacaag
     3181 atgaaaatga aaatatgcta agatggtttt atccaaaagc ctttgagtaa aaatttatac
     3241 aggttaatcc tatttctact tttagttttt tatattttaa attgcaatcg aagagtcaat
     3301 ttaattccaa atcatctata tcaaaaagag aattacaaag aaaatttatt ttcaagaaat
     3361 gaaaaaataa atttaattct aaagattgaa gcagaagaag attttgaact agaagatgtt
     3421 aatttttatt tagcaaatgg gtttcctgaa gaatattcag aagatgcata tgaactagga
     3481 tatctttttc atattccttt tatctataaa gtgaaacaca ttgcatcgat tgattctatt
     3541 tatacttata cagagaaaat tgaaattaaa aatggaaatg tttatgagtt tgtattagaa
     3601 ccaaatacgt attatgcgtt attagaaaga aaaggaggac aagaattttc attcctcgat
     3661 tttaagcgta tcactgtcta ttttggatat attaagaagg gttctctata taataaagta
     3721 aaagaggatg tccattgcca agaaattcag gattctagtt cgcataaggc atatgaaatt
     3781 agttgtcccg ttatttctat tgaagagaat aaaataacaa atatccaaat caaaatagga
     3841 aaaaagaaat ggaattcttt ctttagtatt tttctgaaat ttattccagg aattctattt
     3901 ctaggacctt ttaacaattt accacagcct tattttcatc gagacattcg tattgaaata
     3961 acaaaataga agtaagccat taaactact
//
LOCUS       DAOQOS010000245         3850 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_16053_length_3850_cov_5.423188, whole genome shotgun sequence.
ACCESSION   DAOQOS010000245 DAOQOS010000000
VERSION     DAOQOS010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3850)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3850)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3850
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16053_length_3850_cov_5.423188"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..454
                     /locus_tag="PLF66_26005"
     CDS             <1..454
                     /locus_tag="PLF66_26005"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HMZ67569.1"
                     /translation="CTGAFVAIWYLLQYPDFFETISQEVAKQKYLKTSLNDLDLGKVK
                     KELEDLMLIKKYYQDDEIYLDDVANELMINKNQLSRVLNEIYNKNFYQFINSYRILEA
                     KELIVNFPEKTILDIAFDVGFKSKSAFYKYFTEQTGMTPQEYRKQNRK"
     gene            complement(457..1329)
                     /locus_tag="PLF66_26010"
     CDS             complement(457..1329)
                     /locus_tag="PLF66_26010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67570.1"
                     /translation="MNQRYFYILVLSFLGLFLSCSKLDTIEYSPKLTPESFLSTQHWM
                     RIQFGNFNFVLSQPSSSLIVYFVSLFDIYIAYRFYQTIQNQKSRLYWAIGLFMTGAGA
                     LLAGTSYQAFGYEIKCNGREFCTWTSWWEINYAFLSGLGMNAFLLGASHSNATGSFRK
                     GIQTYAVVNAVLYSILLYFGAFTANWFLVSFEFLSLSSTPSVFFFIWLYGRDYKKEKS
                     QANLYLRNTWIILVAVIVIYAIYLIAGITAPLWKMGIWFTENDILHVGMIYWVYYIGK
                     NLLKEVKDVEANKN"
     gene            1468..2727
                     /locus_tag="PLF66_26015"
     CDS             1468..2727
                     /locus_tag="PLF66_26015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015680754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HMZ67571.1"
                     /translation="MDNKNFFISHDISYPFLWGMDLSLPVILKTALNIDGVDISDEDK
                     LTLRSLNKERLLYFSNHPSTIEPPVAYYVANIIGSRFYYMASRNVFNWGFGVVGELIK
                     RVGAFSVLSGGADRESVKMAKSILAGDRGKLVIYPEGMCSGENDNLVPFMPGIAQIGF
                     WGLEDARKKDPNADIKVLPAFVKYLLSGDENLLYKEIETSIEAIERKLKINPGNKNLL
                     RRFLTVGRILLEDAEREYGISPITDKDYDYRVGAIRHAALNRAAEKLGIKFDPNEDAI
                     HKIREVFTVLDGIHSGLGKQQVKISRKDLEIVQKDVDRAYLFLVIKQEYLLAYPTAER
                     FIEWLYRFENLIFGVTKPRARRARVVIAKPFGLGEYYDSYKENKKKTIEDVTARLRKD
                     IEGLLKDCIPLSKPIVTPFDVGEDLKS"
     gene            complement(2794..2994)
                     /locus_tag="PLF66_26020"
     CDS             complement(2794..2994)
                     /locus_tag="PLF66_26020"
                     /inference="COORDINATES: protein motif:HMM:NF017496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HMZ67572.1"
                     /translation="MYEHPSSAFLVIEVAKSSYLYDLAKLSTYAEANVNQFWILDINT
                     NRLEIYSDPQGNEYKKKQIYYT"
     gene            complement(3118..3297)
                     /locus_tag="PLF66_26025"
     CDS             complement(3118..3297)
                     /locus_tag="PLF66_26025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67573.1"
                     /translation="MASQLLENPTIQNQLVRITREQYYEMAEKYSFGKKTELLEGVVL
                     FKIPKSSIHNFFIDK"
     gene            complement(3340..3708)
                     /locus_tag="PLF66_26030"
     CDS             complement(3340..3708)
                     /locus_tag="PLF66_26030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67574.1"
                     /translation="MQEEYTPEKIIEIINSAAHASLEQTKASKAERSKVPATGFTEKE
                     ISKLLENTANEIVRKEEVDYLANRIKNRFAKIKAISKENFNMFEVAMQCHRLVNDYRA
                     LAKQKGWKEIEAMFSRKENL"
BASE COUNT         1259 a          612 c          697 g         1282 t
ORIGIN      
        1 atgcacgggt gcttttgtgg ctatttggta tttgctgcaa tatccagatt tttttgaaac
       61 gatttcacaa gaagtagcaa agcaaaaata tttaaaaaca agtttgaatg atctagatct
      121 gggaaaggtt aagaaagaat tagaagatct aatgcttata aaaaaatact accaagatga
      181 tgaaatttac ttagatgatg ttgcaaatga actcatgatc aataaaaatc aactttctag
      241 agttttaaat gaaatttata ataaaaattt ttatcaattt atcaattcct atcgtatttt
      301 agaagctaag gagctaatag taaatttccc agaaaaaaca attttggata ttgcatttga
      361 cgttggcttt aaaagcaaat ctgctttcta taaatacttt accgaacaaa ccggtatgac
      421 tccacaagaa tatagaaagc agaatagaaa gtgaggttag tttttatttg cctcaacatc
      481 ctttacttct tttaatagat ttttgcctat ataataaacc caataaatca tgccaacatg
      541 gagaatatcg ttttcagtaa accaaatgcc cattttccaa agaggagcag tgattcctgc
      601 aatgagataa atggcataga taacaatgac tgcaacgagg ataatccatg tattcctcaa
      661 atacagattt gcttgtgatt tttctttttt ataatctcta ccatagagcc agataaaaaa
      721 gaaaacactc ggtgtagagg agagacttaa aaactcaaag gaaactaaaa accaatttgc
      781 tgtgaaagct ccaaagtata ataagatcga atagagaacc gcgttcacta ctgcataggt
      841 ttgaattcct tttcgaaaac tacctgttgc atttgagtga gatgcgccta gtaagaaagc
      901 attcattcca agtccggata ggaaagcata attgatttcc caccaactag tccaagtgca
      961 aaattctctt ccattgcatt tgatctcata accaaaagct tgataactcg ttcctgcgag
     1021 taacgcaccg gcaccagtca taaaaagtcc aattgcccaa tagagtctag acttttgatt
     1081 ttgtatagtt tgataaaagc gataggcaat ataaatatca aataaactaa caaaatatac
     1141 aatcaaagat gaggacggtt ggcttaatac aaaattaaaa ttcccaaatt gaattctcat
     1201 ccaatgctga gtggatagaa atgattcggg agttaatttt ggtgaatact caattgtatc
     1261 taatttactg caacttaaga ataatcccaa aaaggaaagg acaagtatgt aaaaatatct
     1321 ttgattcatg taggcaagtt ttccaattgg atcttttctt gaacagaaaa aatttttttc
     1381 acaaaagtat gattttctaa attgtgtcag attgtaattt atactttttt ttcttgtcaa
     1441 aaagggaagg gcatcctatc taaagctatg gacaataaaa acttttttat atcacatgac
     1501 atcagctatc cttttttatg gggtatggat ttatcacttc cagtaatatt aaaaacagca
     1561 ttaaatatag atggggttga tatttcggat gaagataaac taacccttcg aagcctaaac
     1621 aaagaaagac ttctttattt tagtaaccat ccttccacga ttgagccgcc tgttgcttac
     1681 tatgttgcca atatcattgg atctcgattt tactatatgg cttcgcgtaa cgtctttaac
     1741 tggggatttg gtgttgtggg tgagttaatt aagagagtgg gtgccttttc tgtattgtcc
     1801 ggtggggcag atcgagaatc tgtaaaaatg gctaagtcaa ttcttgcagg agatcgggga
     1861 aaacttgtga tttatccgga aggaatgtgc tcaggagaga atgacaatct tgttcctttt
     1921 atgcctggta ttgcacagat tggtttttgg ggactagaag atgctcgcaa gaaagatccg
     1981 aatgctgata taaaagtttt acctgctttt gttaaatatc ttttatctgg agatgaaaat
     2041 cttctgtata aagaaattga aacttcaatc gaggcaattg agcgcaaact caagataaat
     2101 cccggaaata aaaacttact tcgtagattt ttaactgtag gaaggattct actcgaggat
     2161 gcggaaagag agtatggtat ttctccgatc accgataaag attacgatta tagagtaggg
     2221 gcgatacgac atgcggcatt gaatcgcgca gctgagaaac ttggaatcaa gtttgatcca
     2281 aatgaggatg caattcataa aattagagaa gtatttactg ttctagatgg aattcattcg
     2341 ggtcttggaa agcaacaagt gaaaatttcc cgaaaagatt tggagattgt tcaaaaagat
     2401 gtagatagag catatctttt cttagttatc aaacaagaat acctgcttgc ttatcctact
     2461 gcagagcgtt ttatagaatg gctctatcgt tttgaaaatt taatctttgg ggttacaaaa
     2521 cctagagcca gaagagctcg tgtagtgatt gcaaagcctt ttggattggg cgaatactac
     2581 gatagttaca aagaaaataa aaagaaaacg atagaagatg tgacagctcg tcttcgcaaa
     2641 gacattgaag gattattaaa agattgtatt cctcttagta aaccgatagt tactccattt
     2701 gatgtagggg aagatttaaa gagctgattt tttctcagga aaattaaaga gtcgttcaag
     2761 ggaaatttct cccttgaata tctgaacatt ttctcacgtg taataaattt gtttcttttt
     2821 gtattcattt ccttgtgggt ctgaataaat ttctaatcgg tttgtgttta tatctaaaat
     2881 ccaaaattga tttacatttg cttctgcgta cgttgacaat tttgcgagat catataaata
     2941 agaagacttt gcaacttcta ttactagaaa tgctgaagac ggatgctcat aaatatagtc
     3001 ttctcatttt ccggaaacga cagatatatc tggttctaag tccgaattac caaatgcgat
     3061 agtcttctct gtttgtataa atgaattttc tggtaggatt gcgcttagaa gttgaaatta
     3121 tttatctatg aaaaagttat gaatactact tttaggaatt ttgaataaga caactccttc
     3181 taataattct gtcttctttc caaatgaata tttctccgcc atttcatagt attgttctct
     3241 tgtgattcta actaattgat tttggatagt tggattttct aataattgag aagccataac
     3301 ttgatcgtga tagttagaaa ttatttgtaa agttgctttt tagagattct cttttcttga
     3361 gaacatcgct tcgatttctt tccaaccttt ttgctttgcc agtgctcgat agtcatttac
     3421 caagcgatgg cattgcattg caacttcgaa catgttaaaa ttttctttgc tgatagcttt
     3481 aattttagca aatcgatttt tgattcggtt ggcaagataa tctacttcct cttttcgaac
     3541 gatttcgttt gcagtgttct caaggagctt tgatatttct ttctcagtga agccagtagc
     3601 tggaacttta cttctttctg cttttgacgc cttggtttgt tcaagagatg catgcgcagc
     3661 actgttaata atttctataa ttttctcggg tgtgtattct tcttgcatct aattttcctc
     3721 actgactcct tgcattttac ggaacgaatt aaaaacgtaa actaaaaaat caagactgaa
     3781 tttctttgaa tactttttca acttccgtag gaacagatgt aaaattgact tggaaaatca
     3841 attttacatc
//
LOCUS       DAOQOS010000246         3699 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_17218_length_3699_cov_6.298847, whole genome shotgun sequence.
ACCESSION   DAOQOS010000246 DAOQOS010000000
VERSION     DAOQOS010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3699)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3699)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3699
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17218_length_3699_cov_6.298847"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1125)
                     /locus_tag="PLF66_26035"
     CDS             complement(<1..1125)
                     /locus_tag="PLF66_26035"
                     /inference="COORDINATES: protein motif:HMM:NF026253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HMZ67575.1"
                     /translation="MKSLNLILLFISFFIFECSLFQNKTESDPEIQIETDSSTQTPEI
                     KDDKWEEVASIVKPEPSYKYIALSDKIKFHIEPKFFMVSPFQDKLAYVKYQEKKELKE
                     GYIDASGKIVIATNYDFPFYPFFYGMARVVKDNKTGYLGRNGKIAIPLQFQDAGDFQD
                     TITWFKSKGKYGFINIEGKTIIEPIYDLVESYQEGLACVSLKGKFGYINEKGQIVIPL
                     QFQEAYSFKEGLASVKQNGKFFFINKYGKKAIPLNFDFANSFYEGLARVRINGKYGYV
                     NKKGEWVVKAELDQMYHDAFHFREGRAVFMKGDKYGYLDRNGKVVIEPKFDFADSFSE
                     DLAVVAVNNKVGYINRFGEYVIKPQFENGESFSEGFAAVKK"
     gene            complement(1171..2568)
                     /locus_tag="PLF66_26040"
     CDS             complement(1171..2568)
                     /locus_tag="PLF66_26040"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HMZ67576.1"
                     /translation="MDFPLGINPKTKRPYKAMVIVDSISEKKLLEQNIRAADFQVEEP
                     STEIISFFKPDESTENIEPNNPKPTTEKIDNDSDEIDIDDKEIEEQIKMGKIFAGFSF
                     IDLLFLEYHNYPNSYDKIHKIEKMYPKLIVILILHNVTKNTLDEIVKMKANAYLVKPV
                     SKTVIYDRLKVLLNRKDLLPKIVFGYTNTQGINLTEVQIPPMSEILYKVMLFDSNTVG
                     GSEELEQIVAPDKSLSSDLMRIANSAFYGRSGKINTLKDAITLIGLKTINHIAIVKAR
                     KSFTEKLKNPIFRKYLDEMPILTGLIALDLCAPLHLKTLSDQIFIHSLLYKIGMTILA
                     LNQSERYQKLLTEFQKGKKALYEMEKHEFNTDYLQIGIKVFKIWHLPDSMQDTVKNQE
                     FPIEQLNEVNHLDRLLRLANFFALKLLHHPLNLNDLELMKSILKYYSVDPGFIHLFDE
                     DYYSHIKSHPFFEAF"
     gene            complement(2661..3362)
                     /locus_tag="PLF66_26045"
     CDS             complement(2661..3362)
                     /locus_tag="PLF66_26045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67577.1"
                     /translation="MNLKIMEQVLSFYGGESLWQKSSKVEANVNVKGLAFTLKGRPFF
                     QNAKIEMEINSPVSKITPIGKEESISGILEGDWVYLQNASGKKIAERKNPRSFFPFGR
                     RLFYWDDLDMTYFANYAFWNYFTFPKLLLNSKIEWREKKESVLEGIFPADIPTHSKIQ
                     EFRFDNTGKLLQHNYTAEVISSLAKAANVIHEHGIKNGFPVPSKRIVSPQSLSGNAIG
                     FPVLIDISVLEFNLK"
BASE COUNT         1164 a          600 c          554 g         1381 t
ORIGIN      
        1 ttttttaaca gcagcgaatc cttctgaaaa actttccccg ttctcaaatt gaggttttat
       61 aacgtattca ccaaaacgat tgatgtagcc gactttatta ttcactgcaa cgactgctag
      121 atcttcggaa aaagaatctg caaaatcaaa ctttggttca atgactactt ttccatttct
      181 atctaaatat ccgtatttat ctcctttcat aaatacagct cttccttctc tgaaatgaaa
      241 tgcgtcatga tacatttgat ctaattctgc tttgactacc cattccccct ttttattaac
      301 gtatccatat ttgccattta ttcggactct agctagacct tcatagaaac tatttgcaaa
      361 atcaaagttt agaggtattg cttttttgcc atatttatta ataaaaaaga attttccatt
      421 ttgtttaaca gatgcaagcc cctctttaaa agagtaagct tcttgaaatt gtaaaggaat
      481 tacaatttgt cctttttcat ttatatatcc aaattttcct ttgaggctaa cacaggctag
      541 cccctcttga taggactcaa ctaaatcata aatgggttca attatcgttt ttccttcaat
      601 atttataaaa ccatatttac ctttagattt aaaccatgta attgtatctt gaaaatcgcc
      661 tgcatcttga aattgcagtg gaattgctat ttttccattt cgacctagat agccagtttt
      721 gttgtctttg actactctag ccatcccata aaaaaaagga tagaacggaa aatcatagtt
      781 agttgctatt actatttttc cagaagcgtc aatatatcct tctttcaatt cttttttttc
      841 ttggtatttc acataagcca atttatcttg gaagggactt accataaaga atttaggttc
      901 tatgtgaaat ttaattttat ctgataaagc aatatattta tagcttggct caggtttaac
      961 gatactcgca acttcttccc atttatcatc ttttatttcg ggagtttggg ttgaactgtc
     1021 tgtttcaatt tgtatttcag ggtcggattc tgttttgttt tgaaataagc tacattcaaa
     1081 aataaaaaaa gaaataaata ataagattaa atttaaagat ttcatcaaag ctagaattta
     1141 gtaaaatgtt taactagcaa gaataaaaag ttaaaatgct tcaaagaatg gatggctctt
     1201 aatatgagag tagtagtctt catcaaataa atgaatgaat cctggatcga ctgagtaata
     1261 ttttaaaata cttttcatta attccaagtc atttagattt aaaggatggt gaagtaattt
     1321 tagggcaaaa aagtttgcaa ggcgcagaag tctgtctaga tggtttactt catttaattg
     1381 ctctattggg aactcttggt tttttactgt atcttgcatc gaatcaggta ggtgccatat
     1441 tttaaaaact ttaataccaa tttgcagata atctgtatta aactcatgtt tctccatttc
     1501 atataaagct tttttccctt tctgaaattc tgttaaaagt ttttgatatc tttcggattg
     1561 gtttaatgcc agtatagtca ttcctatttt atataaaaga gaatgaatga atatctgatc
     1621 gcttaacgtt tttaaatgta aaggagcgca taaatcaaga gcaatgagtc cagttagtat
     1681 tggcatttca tctagatact tcctaaagat cggattcttt aatttttctg taaaactttt
     1741 acgtgctttt acaatcgcta tatgattgat tgtttttagt cctataagag ttatcgcatc
     1801 ttttaaagta tttatttttc ctgaccttcc atagaaagca gaattcgcaa tcctcataag
     1861 atctgaactt aatgatttat ctggagcaac gatttgttct aactcttcgc ttcctccgac
     1921 agtatttgag tcaaatagca ttactttata taaaatctct gacataggag gaatctgcac
     1981 ttcagttaga ttgattccct gggtatttgt atatccgaaa acaattttag gtaaaagatc
     2041 ttttctattg agtaaaactt ttaatctatc atagatgact gttttggaaa ctggctttac
     2101 aagataagca tttgccttca tttttactat ttcatctaac gtattctttg ttacattatg
     2161 taatattagt attactatta atttaggata catcttttcg attttatgaa ttttatcata
     2221 actgtttggg taattatgat attctagaaa tagcaaatca ataaaagaga aaccggcaaa
     2281 aattttgccc attttaattt gttcttcaat ctctttatca tcaatatcaa tttcatctga
     2341 atcattgtct attttttcgg ttgtaggctt aggattgttt ggctcaatat tttcagtcga
     2401 ttcatcaggt ttgaaaaaag aaataatctc tgtcgaaggc tcttccactt ggaaatcagc
     2461 agctcttata ttttgctcca ataatttttt ttcggagatg gaatctacga ttaccattgc
     2521 tttatagggt ctcttcgttt ttgggtttat acctaaaggg aaatccattt atctctctga
     2581 cctttcctaa tgacaagaat gggaaatgga acctaaactt tctagtaaaa aaatttttga
     2641 tttttcaatt ctttattttc ttattttaag ttaaactcaa ggacgcttat atcgatcagg
     2701 actggaaatc cgattgcatt tccgcttagg gactgcgggc tcacaattcg ttttgatgga
     2761 acagggaacc cattctttat tccatgttcg tgaatcacat ttgctgcctt tgcgagactg
     2821 ctaattactt cagctgtata attatgctga agcagtttgc cagtattatc aaatctaaat
     2881 tcttggattt tactatgcgt aggaatatcg gctggaaaga ttccctctaa tacagattcc
     2941 tttttttctc tccattcaat tttggaattc aaaagtaatt ttggaaaagt aaaataattc
     3001 caaaatgcat aatttgcaaa ataagtcata tccaaatcat cccaataaaa gagtcttctt
     3061 ccaaagggaa agaaacttct tggatttttt ctttctgcaa ttttttttcc agaagcattt
     3121 tgtaaataga cccagtctcc ctctaagatt ccagaaatcg attcttcctt tccgattggc
     3181 gtaatttttg atactggaga attgatttcc atttcaattt tcgcattttg aaaaaaaggt
     3241 cttcctttta gagtaaaggc gagacctttt acatttacgt tagcttctac tttagaagac
     3301 ttttgccaaa gagattctcc accataaaag gaaagaactt gttccattat ttttaggttc
     3361 attgtattag tttttaaagc agagttaagt cggcaatcaa aagagaaaaa acactaaaga
     3421 tatattttta ttctttaact cactttatac actgtagtca ttcaaatatt tatttgtatt
     3481 aaggtttaat cttcaaatga gttttgcttt taataccaat caaaaatgaa attaggtttg
     3541 agtgaataaa aaaaattaca aagaagaatc tttatgcatt tctatataag atgttcggta
     3601 gagaaaatct cagaggctaa tgacttgatt agtaaattag aattttcgta acctatcagg
     3661 gtgatgaaat aaaaaattaa aggaaggaaa caaagggta
//
LOCUS       DAOQOS010000247         3566 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_18356_length_3566_cov_6.438906, whole genome shotgun sequence.
ACCESSION   DAOQOS010000247 DAOQOS010000000
VERSION     DAOQOS010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3566)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3566)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3566
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18356_length_3566_cov_6.438906"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..812
                     /locus_tag="PLF66_26050"
     CDS             <1..812
                     /locus_tag="PLF66_26050"
                     /inference="COORDINATES: protein motif:HMM:NF019307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidase dimerization domain-containing
                     protein"
                     /protein_id="HMZ67578.1"
                     /translation="ITAEKGSLAGINTEVAMIGVAEKGFLAAKIIVKGKGGHSSMPPS
                     ESAMGNAAKLMLEFEKNQIPQRLTPIIQSFFQNVGGSMSFPTRFSIANQWILSPLLLR
                     QLANSPAGNAISRTTTALTMMKGSDGANVISPEVEFVVNFRILPGDTVEDVRNHIKKI
                     TQGYDIVIEEIGNTRGASIVSRTDTKPYKSLEKVIQAIYPNAIVTPYITIGGTDAFKY
                     EKLSENIFRFNPVRLDAEERASIHNFNESIRVENFQRTIHYYRLLIQELNQ"
     gene            complement(1307..2677)
                     /locus_tag="PLF66_26055"
     CDS             complement(1307..2677)
                     /locus_tag="PLF66_26055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HMZ67579.1"
                     /translation="MELKRTLSLTDSVSLMFSSMVGSGVFFTTGFILNKVENPWLVIT
                     CWVLGGIFAIAGAVTFAYPAVIFPEAGGDYIYLKKAYSPLVAFMSGWASLTVNFSASI
                     SVLGIAFSKYLQYIFPSLKDLPSFKTHIGPIPMEIGTTQLIGAGLITFFSVINYFGIK
                     QAVRLQNVFTSIKISGLLILVIAGFAFGKTDFTPLHTNSFFPDIFQNVNLLILGIVPV
                     SFSYLGWNMVTYVAGEIKDPRRIIPLSIAIACIMVMSLYICINLLFLVSAPISELKGQ
                     GGIGVIAAKHLFGEGITTIISIFICWIILGSMSAMIIGGSRIYYAMANDGLFFHHLAD
                     LHPKYASPYKALIFQCVYSSILILFNQLEDLLYFITCAILFLSSLTAFIPFVLKKEYK
                     NTSDYKIPLYPLPPLLYIIANAVLITILAYEQPMNAVKGIGITLLAIPVYYLFSHTFK
                     KPVEGK"
     gene            2711..>3566
                     /locus_tag="PLF66_26060"
     CDS             2711..>3566
                     /locus_tag="PLF66_26060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67580.1"
                     /translation="MSNKFLIIFLFYFFILSFCKVDKTSGESIPVVETPESKALQMKS
                     LFLSGKLQADDTSKLGKITRTSYYATFRQRITKHWEQYNKEIQDPFVEWKNKNVPEPK
                     SKVVFYPFSGPDFPNAFTIYPKAQTYIMIGLEAGGFDPDIENMNENQIARGLAELNGS
                     LDTISRLNYFMTNSMKQDVSKSVFRGTAPVFLAYFGFLKVKPYSIKNFYLNDNGDIIY
                     LSKEDIKANANYREGLVSLDIDFLDPVDGSKKKLYFLSTNISNFGLKANPGTMKFLGK
                     FGMFASTFK"
BASE COUNT         1293 a          632 c          628 g         1013 t
ORIGIN      
        1 gaatcactgc agagaaagga agtcttgctg gaatcaacac ggaagttgct atgattgggg
       61 ttgcagagaa aggcttctta gccgctaaaa taattgtaaa gggcaaaggc ggacattcct
      121 ccatgcctcc ttctgagtca gctatgggaa acgcagcaaa actcatgctt gagtttgaga
      181 aaaaccaaat tccacagagg ctaacaccta ttatccagtc cttctttcaa aatgtgggtg
      241 gttctatgtc ttttcctact cgcttttcta ttgcaaatca atggattctt tctccattgc
      301 tactacgcca acttgcaaat agcccagcag gcaatgcaat cagtcgaact actactgcac
      361 ttacaatgat gaagggaagc gatggcgcaa atgttatttc tccagaagtt gaatttgttg
      421 tgaattttag aattcttcca ggagatacag tagaggatgt aagaaatcat attaaaaaaa
      481 ttacccaagg gtatgacatc gtaatcgaag aaatagggaa cacaagaggc gcatccattg
      541 taagtagaac cgatacaaag ccctataaat ctcttgaaaa agtgattcag gcgatttatc
      601 ccaatgcaat tgtaactccc tatatcacga taggcggaac agatgcattt aaatatgaga
      661 aactaagtga aaatattttt cgcttcaatc cagtccgatt agatgcagaa gaaagagcaa
      721 gcattcataa tttcaatgaa tctattcgtg tagaaaactt ccaaagaacg attcactact
      781 acagacttct cattcaagaa ttgaatcagt gatttgtaaa aggattacca ccgatgaaca
      841 ccgatcgctt cgctaacacg gataaaagat acgattgatt atcggaagaa aatagataat
      901 tcgattgagt ggatattctt tgttatccgc agattactaa cgaagaactc aaaaaaagac
      961 taccacggat aaacacggat cgcttcgcta acacggataa aagatacgat tgattagcag
     1021 aagaaaatag gtaaatcgat tgtttggata ttctttatca cgctaagacg cagagagatt
     1081 tatttctcag ctccaatcta tttcgatacc agcacgaatt ttaaaatatt cattctggta
     1141 gaaaacacaa ctccggcgaa accatcgaag taattcatgt cggctgcaat ttatttttat
     1201 cagaatagac caaaatagta ttttctatcg gtgtcctcgg tgcaagtcgg tggtaatctt
     1261 tttctccaaa gccctaattt tacttagggc ttagacgaac tcgaaactat ttcccttcta
     1321 ctggtttttt aaatgtatga ctgaataaat aataaacagg gatagcaagt aacgtaattc
     1381 caattccttt cactgcattc attggttgtt cataggcaag aattgtaatt agaactgcat
     1441 ttgcaatgat gtaaagaagc ggtggaagag gatagagtgg aattttataa tcagatgtat
     1501 ttttgtattc ttttttcaga acaaaaggaa taaaagcagt cagagaagat aaaaataaaa
     1561 tagcacatgt aataaaataa agtaggtctt ctaactgatt aaatagaatc aagatagaag
     1621 aatagacaca ttgaaaaata agtgccttat aaggacttgc atattttgga tgaagatctg
     1681 ccagatgatg gaaaaacaaa ccgtcatttg ccatagcata ataaattcta gaaccaccaa
     1741 taatcattgc agacatagaa cctaaaatga tccagcagat aaaaatagag atgatcgttg
     1801 tgatcccttc tccaaataga tgtttagccg caatcactcc aatcccacct tgtcctttta
     1861 attctgaaat aggagcagaa actaaaaaca aaagattaat acatatataa agagacatga
     1921 ccatgataca ggcaatggca atcgaaagag ggatgattct tctaggatct ttgatctcgc
     1981 ccgcgacata ggttaccatg ttccatccta gataggaaaa agaaacaggc acaattccaa
     2041 gtataagtaa atttacattc tgaaaaatat ccggaaaaaa agaatttgta tgaagtggtg
     2101 taaaatcggt ttttccaaat gcaaaaccag caatcactaa tatgagtaag ccagatattt
     2161 taatggaagt aaaaacgttc tgaagtctga ccgcttgttt aatcccaaaa taattgatca
     2221 cactaaaaaa agtaatcagc cctgctccaa tcaattgagt cgttccaatc tccattggaa
     2281 tgggaccaat atgagttttg aaacttggta ggtctttaag gctcggaaat atatattgta
     2341 aatattttga aaaagcaatc cccaaaacag aaatacttgc agaaaagtta accgtaagag
     2401 atgcccagcc actcataaag gcaactaagg gggaataagc ttttttgaga taaatataat
     2461 ctccccctgc ttctggaaaa atgacagccg gatacgcaaa tgtaaccgca ccagcaattg
     2521 caaagatacc acctaaaacc caacaagtga taactaacca aggattttcc actttgttta
     2581 gtataaatcc agtcgtaaaa aaaactcccg aaccaaccat tgaactaaac atgagtgaaa
     2641 ctgaatctgt taaagaaagt gttcgcttta attccataaa tgaatatttt gactataaaa
     2701 aaggacgtac atgagtaaca agtttttaat tatattttta ttttattttt ttatcctgag
     2761 cttttgcaaa gtagataaaa cttctggcga aagtattcca gtcgtagaaa cacctgaatc
     2821 aaaagctctt caaatgaaat cacttttctt aagtggtaaa ctacaagcag acgatacaag
     2881 caaactaggt aaaattacac gcacttctta ctatgcgact tttcgccaac gaatcacaaa
     2941 acattgggaa caatacaaca aagaaattca agatccattt gtagaatgga aaaataaaaa
     3001 tgttccagaa ccaaaatcaa aagttgtctt ttatcctttt tctggtcctg attttccaaa
     3061 tgcatttacc atttatccaa aagctcaaac ctatatcatg attggacttg aggcgggtgg
     3121 gtttgatcca gacatagaga acatgaatga aaatcaaatt gcaagagggc ttgcagaact
     3181 caatggaagt ttagatacaa tctctagatt gaattatttc atgacaaatt ctatgaagca
     3241 agacgttagt aaatctgtct tccgtggaac agctcctgta tttcttgctt actttggctt
     3301 tttaaaagta aaaccttatt ctattaagaa tttctatctc aatgacaatg gagatattat
     3361 ttatctttca aaagaagaca ttaaagcaaa tgcaaattat agagaaggtt tagtaagttt
     3421 agacatagac tttcttgatc cagtagatgg ttcaaagaaa aaactttatt tcttatctac
     3481 aaatatatct aatttcggtc tgaaagcaaa tcctggaaca atgaagttct tgggaaaatt
     3541 tggaatgttt gcctctactt ttaaag
//
LOCUS       DAOQOS010000248         3510 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_18883_length_3510_cov_6.382634, whole genome shotgun sequence.
ACCESSION   DAOQOS010000248 DAOQOS010000000
VERSION     DAOQOS010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3510
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18883_length_3510_cov_6.382634"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            223..1137
                     /locus_tag="PLF66_26065"
     CDS             223..1137
                     /locus_tag="PLF66_26065"
                     /inference="COORDINATES: protein motif:HMM:NF019845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HMZ67581.1"
                     /translation="MKAVICESYGEPNVLKLKDIEKPIPKPNEILIRIRATAVNSADV
                     RVRGLQVSGIKRILMYLVLGLTKPRKKILGVVLSGVVEEVGNNVQTFKKGDEVFGMTG
                     FQFGTYAEYISLKEKSAVCLKPKNANFQEAASIIFGGSTAYYFLKKAKIEELPKKVLI
                     YGATGSVGISAIQIANYFGADVSAVCGTNGIGLVKSLGVKSVYDYNSDWIHSMQDRFD
                     VIFDAVGKLSQKQCEGILRPGGKFFTVEGWDTANETIEQLEFLKELFENGKLKAMIDR
                     VYKLDQIVEAHTYVDTGRKKGNVIIEID"
     gene            complement(1184..3253)
                     /locus_tag="PLF66_26070"
     CDS             complement(1184..3253)
                     /locus_tag="PLF66_26070"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HMZ67582.1"
                     /translation="MLRIISFCFAFLIPSCSRMQTTKINAIKGVIDLQSVSFDQKSIY
                     LDGEWEFYPSTFLNPTEKPNHTQYIRVPSSWNDEMKSSYGYASFRLKVLLPKDQKELL
                     AFKIPEQGTSYRIFVNGVLLANNGTVGVNSSESKPEFFPIVTKGFVAEKEIDIILHIS
                     NFEHREGGFWYSILLGKEENIKQVREDALLLDLFLCGSILIMGAYHIGLFIIRKKDLS
                     PLYFGLFCFVIIFRLLSQSEKYLVQIIPGISFRTLNQTEYISYFLALPSFSSFICSLF
                     PKEFPRKILIAIWIIAFSFCGIVLFTSTNFYTSMVNYYHLFTLICIVLILYTFVKAWI
                     NKRESVLMLFIGTMLLIAGTVNDILHSIEVIHTSYIVPHSLLAFIFVQSIILSIRFSK
                     AFYENEKLIDEMTQLSGIQKRFVPKEFLENLGKKNFSEVKLGDQIQKQMTILFSDIRD
                     FTSLSEHMNPKENFQFINEYLSRMGPLVRKQNGFIDKFIGDAIMALFNSPDEAIQSAI
                     DMQKELTSMNESLKRLGKKTIDVGIGIHTGSLMMGVIGEEERFEGTVISDAVNVASRI
                     EGLTKYYHSKILVSEDVLLGLKEDFTFTTMKIDKVKVKGKSIEFAIYEILDGLNPKEL
                     DLVLQTKLAFETGVECYIQKELTEAKLLFEKVLSVYPSHSSAQIYLKRCVNQLNDEHS
                     IDSQRLD"
BASE COUNT         1229 a          531 c          618 g         1132 t
ORIGIN      
        1 agaacgttat gagcatgagt acatagcaag atactattct gcaaagaaac taacaaaagt
       61 tatatcaaaa gattttcctc cttgttttgg tttttttgct tatccaccaa attgtatgaa
      121 tggtgaattc tcagagggac tcgcatgggt cgcaaaaaaa ttatagttaa atttttattt
      181 tagatcaaca caaaggatac aaaaaggaga taaaggacat ttatgaaagc agtaatttgt
      241 gaatcttacg gagaaccaaa tgttttaaaa ttaaaggaca tcgaaaaacc aataccaaaa
      301 ccaaatgaaa tattgattcg gattcgagca acggctgtga attcggctga tgtaagagtg
      361 agagggttac aggtatctgg tataaagaga attcttatgt atcttgtgct tggattgaca
      421 aaacctagaa agaaaatctt aggagttgta ttatcaggag ttgtggaaga ggttggaaat
      481 aacgttcaaa ctttcaaaaa aggcgatgag gtttttggaa tgacaggatt tcaatttgga
      541 acttatgcag aatatatttc tttaaaagaa aaatcagcag tctgcttaaa acccaaaaat
      601 gcaaactttc aagaagcagc ttcaattatt tttggaggat ctactgctta ttatttttta
      661 aagaaggcta aaatagaaga gctcccaaaa aaagttttaa tctatggagc cactggatct
      721 gttggaattt ctgctattca aattgcaaac tatttcggag cggacgttag tgctgtatgt
      781 gggacaaatg gaataggtct tgtaaagtca ctcggagtca aatcggtata cgattataac
      841 tcagattgga ttcacagtat gcaagataga tttgatgtga tttttgatgc tgttgggaaa
      901 ctatctcaga aacaatgcga aggaatcttg cgtccgggtg gaaaattttt tacagtagag
      961 ggttgggata ctgcgaatga aacaatagaa caactagagt tcttaaaaga gttgtttgaa
     1021 aacggtaaat taaaggcaat gatcgataga gtttataaat tggatcaaat tgtagaagca
     1081 catacctatg tagacacagg tagaaaaaaa ggaaatgtaa tcattgaaat agattaaaga
     1141 ctaactctaa aaggattata gaaacagagt gtttctaaaa gaattagtct aatcgctgtg
     1201 agtctatgga atgttcatcg tttaattgat tcacacaacg tttcaaataa atctgcgcag
     1261 aagagtgtga cgggtaaaca ctcaatactt tttcaaaaag taatttcgct tctgtgagct
     1321 ctttttgaat ataacattca actcctgttt caaaagccag ttttgtttgc agaactagat
     1381 ctaattcttt tgggttaagt ccatctagaa tttcataaat tgcaaattct attgattttc
     1441 cttttacttt tactttatca attttcatag tcgtaaaggt aaaatcttct ttgagtccta
     1501 agagcacatc ttcactcact agaattttag aatgatagta tttcgtaagt ccctcgattc
     1561 gagacgcgac attgactgca tcactgatca cagttccttc aaatctttcc tcttcaccaa
     1621 ttacacccat catcaaacta cctgtatgaa ttccaattcc tacatcaatt gtttttttcc
     1681 ccaatcgttt taaggattca ttcatagaag ttaattcttt ttgcatatca atagcagatt
     1741 gtattgcttc atccggagaa ttaaacagtg ccatgatcgc atccccaatg aatttatcta
     1801 taaatccatt ttgctttcta actaacggtc ccattctaga aagatattca ttgataaact
     1861 gaaaattttc tttcggattc atatgttcag aaagacttgt aaagtctcgt atatcagaaa
     1921 agagaatggt catctgtttt tgaatctgat ctccaagctt cacttcagaa aaatttttct
     1981 ttcctaaatt ttctaaaaac tccttaggaa caaatctctt ttgtattcca ctgagttggg
     2041 tcatttcatc gattagcttt tcgttttcat aaaatgcttt agaaaatcga atcgataaaa
     2101 tgatagattg cacaaagata aatgcaagta aggaatgagg aacgatatag cttgtatgaa
     2161 tcacttctat agaatgaaga atatcattta cagtgcctgc aattaaaagc attgttccga
     2221 taaaaagcat tagaacactt tctcgtttat tgatccatgc ttttacaaaa gtgtatagaa
     2281 tgagcacaat acaaatcaaa gtaaataggt gataataatt taccatgctt gtgtagaaat
     2341 ttgtagaggt aaaaagaaca attccacaaa aactaaaagc aatgatccaa atcgcgatca
     2401 gtatctttcg aggaaactct tttggaaaaa gagaacaaat aaaactagaa aaactaggaa
     2461 gagctaaaaa ataacttata tactcagttt ggtttaacgt tctaaaagag attccaggaa
     2521 tgatttgaac taaatatttt tctgattgag aaagcagtcg aaaaataatt acaaaacaga
     2581 agagtccaaa atacaaagga gataaatctt tttttcgaat gataaaaagt cctatatggt
     2641 aggcgcccat gatcaagata cttccacaaa gaaatagatc gagtaggaga gcatcctctc
     2701 tgacttgttt tatattttct tcctttccta aaagaatgga ataccaaaaa cctccctctc
     2761 tatgttcaaa attagaaatg tgtaggatga tatcgatttc tttttcggct acgaagcctt
     2821 ttgttacgat tggaaaaaat tccggtttac tttctgaact attgactcct acagttccat
     2881 tattggcaag caagactcca tttacaaaga tccgatagga tgtgccttgt tctggaattt
     2941 taaatgcaag taactctttt tggtctttgg gtaataatac cttaaggcga aaactagcat
     3001 atccatagct acttttcatt tcgtcattcc aagaactggg aactcttata tattgagtgt
     3061 ggtttggctt ttctgttgga tttaaaaaag tagaaggata aaattcccat tctccatcta
     3121 ggtaaatcga tttttgatcg aatgaaacag attgtaaatc tatcactccc tttattgcat
     3181 ttattttagt tgtttgcatc ctagaacagg aagggattaa aaatgcgaaa cagaaagaaa
     3241 tgattcgaag cattgaaagg atcgtttttg aattcgattg attttttttc atgagtataa
     3301 atctgtttta tttctggtaa gattcagagt attattgtta caaagcaaca tgaattttaa
     3361 aatttatttc attcgattga tattttattc acagcattta ttcagattta aaaaaaaaga
     3421 ttctagtgaa tgaggatttt tagaatatta gagaatgcgt aacctatcag ggtgatgaaa
     3481 taaaaaatta aaggaaggaa acaaagggta
//
LOCUS       DAOQOS010000249         3486 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_19130_length_3486_cov_2.864762, whole genome shotgun sequence.
ACCESSION   DAOQOS010000249 DAOQOS010000000
VERSION     DAOQOS010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3486)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3486)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3486
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19130_length_3486_cov_2.864762"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            360..1982
                     /locus_tag="PLF66_26075"
     CDS             360..1982
                     /locus_tag="PLF66_26075"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HMZ67583.1"
                     /translation="MQKLNTHKGQAVLFQKDDFLTIKEASQWATEHLGKTVTSSNISY
                     LIQYGRISKIGTNGSTQISKEELLKYYESYNGKRQLAWKDQLGEDLNWALSFDEYTEA
                     ETTKHVHRLHPYKGKFIPQLVEYFLDSHTDDFKREVYFKAGDIVLDPFSGSGTTMVQA
                     SELGIHAIGLDISEFNTRIGNSKVTDYNLLDVETELHRITKALKVFLSDSNTIEFEET
                     LLRELYEFNQKYFPVPEYKYKVQRHEINEDYYGHEKEKEFLPIYNKLVKKYNIKLRQE
                     KSDTFLDKWYSAHIRNEIDFVHKEIKRIKNPDTKNIISIILSRTIRSCRATTHADLAT
                     LKEPVTTSYYCAKHGKVCKPLFSILKWWETYSKDTLKRLEQFKQLRSKTFQTCLTGDS
                     RSIDIFKELEAVNEPFAKLARKKKINGIFSSPPYVGLIDYHEQHAYAYDLFGFKRKDN
                     LEIGPMFKGQGREAKENYIKGISDVLNNCKQYLVKDFDVFLVANDKYGMYPTIAENAG
                     MQIVNQYKRPVLNRTEKDKAAYAEIIFHLKRK"
     gene            1986..2156
                     /locus_tag="PLF66_26080"
     CDS             1986..2156
                     /locus_tag="PLF66_26080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67584.1"
                     /translation="MNATMEDLLRRDQVDLDIEINDTFISCVYGELEYTIEGDCGIVN
                     TISVYHRTREGI"
     gene            2166..2381
                     /locus_tag="PLF66_26085"
     CDS             2166..2381
                     /locus_tag="PLF66_26085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67585.1"
                     /translation="MNQFELFAISEGVKIIEDPVSPTQEVILFWFSLGYEPATSEAMK
                     WANKISRSYREREWDIPSGVVLMSKKL"
     gene            2419..>3486
                     /locus_tag="PLF66_26090"
     CDS             2419..>3486
                     /locus_tag="PLF66_26090"
                     /inference="COORDINATES: protein motif:HMM:NF022062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIPR family protein"
                     /protein_id="HMZ67586.1"
                     /translation="MQDLVITGNLNSFKDNFGFENLKESEQFERFCFFSLLNREVRYA
                     LNPNDIDSISVGENKGIDGIAIVINDGLVKSQQEFEQVIQSKQKISVVIYFIQAKTST
                     SFDDKEMANFLDTINDFLEEKPLYPTTKEIQSYHGIYLMMMNNLRIVKDFQLYAFYAC
                     AGNWNENTSISTTIQIKKKILEGRNLFSQVNIIPVDKTKLLDFYRKASNPIEAEFSFN
                     QKVSISGIDSVDESYIGLLSFKEFKKLIIEPNKDRIRNLFYDNVRDFLGTENEVNDKI
                     QDTIRNKKFLEFALMNNGITIIASENSGRGDTFILHNYQIVNGCQTSNVLFECKDFPG
                     IDDTLIPVKVIVTQNEVLRDKI"
BASE COUNT         1266 a          535 c          635 g         1050 t
ORIGIN      
        1 taacctccag ccaatgactt tactttgttc tcggaataaa ctctaacctg ttcaactacg
       61 taattatttt aactttttta ggattgtgat actgtttcat ttgacatgtt ttttgtttgg
      121 aaaaaagtca gtcagggttt gaatgctatg gctggagatt aatttcaaat aggaaaaaat
      181 tggacaagat gaataatatc tactggatca gcgtttatta aaacaatagg taaaatttta
      241 ttactagatt agcctttgtt aagttaaact gaacagaaat tcaggttctt ttttaattga
      301 caataggatt agacataatt cataatttta aagcaaggta aagtaagtgc ggggctacaa
      361 tgcaaaaact aaatactcat aaagggcaag cagttttatt ccagaaagat gattttctaa
      421 ccattaagga agcaagtcag tgggctaccg agcatcttgg aaaaactgtc acctcttcca
      481 atatttctta cctcatacaa tatggtcgta tttctaaaat aggcacaaat ggctctaccc
      541 aaatttccaa agaagaactg cttaaatatt acgaatccta caacggcaaa cgccaattag
      601 cctggaaaga ccaattgggc gaggatttga actgggcctt gtcgttcgat gaatacacag
      661 aagcagagac tacaaagcat gtgcatagac ttcaccctta taaaggaaaa ttcattccac
      721 agttagtaga atatttttta gactcgcata ctgacgattt caaacgagaa gtttatttta
      781 aagcaggaga cattgttctt gatccatttt cgggaagtgg gactacaatg gtgcaagcat
      841 ctgagcttgg aattcatgca attggtttag atatttctga atttaatact cggattggaa
      901 attccaaagt tacggattac aacctattgg acgttgagac agaacttcat cgaattacaa
      961 aagcactcaa agtattttta tcagattcaa atacaataga atttgaagag actttattaa
     1021 gagaattgta tgagtttaat caaaaatact ttcctgtccc agaatacaaa tacaaagtcc
     1081 aaagacacga aatcaatgaa gactattacg gtcatgaaaa agaaaaagaa tttctaccga
     1141 tctacaataa acttgtaaag aaatacaata ttaagcttcg ccaagaaaaa tccgacacct
     1201 ttctggataa atggtattct gcgcatatca gaaacgaaat cgattttgtt cacaaagaaa
     1261 ttaagagaat aaaaaatccg gatacaaaga atataatcag tattatttta agcagaacca
     1321 ttcgctcttg tcgcgcaacg actcacgccg acttagcaac acttaaagag ccagtaacaa
     1381 ctagctatta ttgcgccaag catggaaaag tatgcaaacc gttattctcc attttaaaat
     1441 ggtgggaaac ttattctaag gatacgctga aacgattaga gcaattcaaa caacttcgct
     1501 ctaaaacatt tcaaacttgt ctcacaggcg atagtcgctc tatagatatt tttaaagaac
     1561 tagaagcggt aaatgagccg tttgcgaaac tcgcacgcaa gaaaaaaatc aacggaattt
     1621 tttcaagtcc tccttatgtt ggtttaattg actaccacga acaacacgct tacgcctatg
     1681 atttatttgg ttttaaacgc aaagataatt tagaaatagg acctatgttt aaaggacaag
     1741 gcagagaggc taaagagaat tatatcaaag gaatttcgga tgtcctgaac aattgtaaac
     1801 aatacttagt caaagacttc gatgtatttc tagtggctaa tgataaatac ggaatgtatc
     1861 caacgattgc ggagaatgcc ggaatgcaaa ttgtgaatca gtataaacgt cccgttctaa
     1921 atcgcacaga gaaggataag gcagcttatg cggagattat ttttcatttg aagaggaagt
     1981 aggatatgaa tgctactatg gaagatttat tacgcagaga tcaagttgac ctagatatcg
     2041 aaattaatga tacatttatt tcgtgtgtgt atggtgaatt ggaatatacc atcgaaggcg
     2101 attgcggaat cgtaaacaca atcagtgttt accatcgaac tagagaagga atttgaacac
     2161 aacttgtgaa tcaatttgaa ttattcgcga tttcagaagg agtcaagatc attgaagatc
     2221 cagtatcccc cacacaagaa gtaattttat tttggtttag tttgggatat gaacccgcaa
     2281 ccagcgaagc tatgaaatgg gcaaataaaa ttagccgaag ctatcgagaa agagaatggg
     2341 atattccttc tggtgttgtt ttgatgtcga agaagttgtg agattttttt aagtttttaa
     2401 cgctaataag gagattttat gcaggattta gtaattacag gcaacttgaa ttcatttaaa
     2461 gataattttg gctttgagaa tttaaaagaa tcagagcaat ttgaaagatt ttgttttttt
     2521 tctttgctga atcgtgaagt ccgatatgca ttaaatccaa atgatataga ctcaatttca
     2581 gtaggagaga ataagggtat cgatggaatt gcaattgtaa ttaatgatgg gctcgtaaaa
     2641 tcacagcaag aatttgagca ggttattcaa tctaagcaaa aaatttcagt agtaatatat
     2701 tttatacaag caaagacttc tacatctttt gatgataaag aaatggcaaa ttttcttgat
     2761 actatcaatg atttccttga agagaaacct ctttatccaa ccacgaaaga aattcaatct
     2821 tatcatggaa tttatctgat gatgatgaat aatttacgaa tagtgaaaga ttttcaactt
     2881 tatgcttttt atgcatgcgc aggaaattgg aatgagaata ctagcatttc aacgacaatc
     2941 caaataaaga agaaaatttt ggaaggtaga aatctttttt cccaagttaa tattattccc
     3001 gtggataaaa ctaaactatt agatttttat agaaaagcat caaatccgat tgaagcagaa
     3061 ttctcgttta accagaaagt ctctattagc ggaatcgata gtgtcgatga gtcctacatt
     3121 ggattattat ctttcaagga attcaaaaaa ttaatcatcg aaccgaataa ggatagaata
     3181 agaaatcttt tttatgacaa tgttcgagat tttctaggaa ctgaaaatga agtaaatgat
     3241 aaaatacaag atacaatacg aaataaaaaa tttttagaat ttgcattaat gaataacggt
     3301 attacaatca ttgctagtga aaattcaggc cgtggggata cttttattct gcacaattat
     3361 caaattgtaa atggatgcca gacttcaaat gtcttatttg agtgtaaaga tttcccaggg
     3421 attgatgata ctttaattcc agtaaaagta atagtaactc aaaacgaagt attacgagat
     3481 aaaata
//
LOCUS       DAOQOS010000250         3485 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_19134_length_3485_cov_5.814286, whole genome shotgun sequence.
ACCESSION   DAOQOS010000250 DAOQOS010000000
VERSION     DAOQOS010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3485)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3485)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3485
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19134_length_3485_cov_5.814286"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..241
                     /locus_tag="PLF66_26095"
     CDS             <1..241
                     /locus_tag="PLF66_26095"
                     /inference="COORDINATES: protein motif:HMM:NF023283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3375 family protein"
                     /protein_id="HMZ67587.1"
                     /translation="DELISNIDSLLKYKPKVELKEVIEKYPLKKGLEELVTYLWIASN
                     REQHFVDSTKTEEYSIHIDDEKTVSIKFPQVVYKI"
     gene            241..822
                     /locus_tag="PLF66_26100"
     CDS             241..822
                     /locus_tag="PLF66_26100"
                     /inference="COORDINATES: protein motif:HMM:NF025205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4194 domain-containing protein"
                     /protein_id="HMZ67588.1"
                     /translation="MEHTLPYAAAVLKLLKGPVNYDLDPKEWDQLATYQTDIKKYFDK
                     IGLHLVVDNDDGFAYLYQPSGDDDLGLPRITRRIPLPFDITLLLVLLREKLTEQAIDD
                     RNLANLLKKEDLYELLNPFYPKTQNESLQYREYDRIISKVVELGFLRKTEKDKNEFYQ
                     IEKIIKVRIRAEELNTIKARLLEDNDRKTVQDD"
     gene            794..>3485
                     /locus_tag="PLF66_26105"
     CDS             794..>3485
                     /locus_tag="PLF66_26105"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HMZ67589.1"
                     /translation="MTERLFKMTDINTDMGYRLLRMEFLNWGTFSNGKIFAVDTNGKT
                     ALITGANGSGKSTLVDALLTLLVPSQKRNYNLASGTERKRDRDEKTYIQGNYGKTTDE
                     TQGSKILKMRDPSEGHISVLLAVFYSEEIKRYASISQVLWFNNGNLEKFYVVAERELN
                     IKDDFKHKGDIKNFKKVLKNQENIKVFDSFKEYIHEFIKNVGLRSEKALDLFNQIVAI
                     KSIGNLKEFIREHMLEKHDVNELIEDLDKNYKNLNDSYNAILQARRQLEELIPIEEES
                     VKYEKIVEEIHNIQGSIFILPAFFAREKINLLKENLESTKFNLEKMNDIVESNEKEII
                     RKREEERSLYATLENDSIGRRITELQKQLIDERKILVGRQSAYDKYKNLAASLLLQTK
                     VTQENFYENKKLAEEKIHKNLDLLKEIEKKRDHWVVVRNTLHNQKQELESELNSLKTR
                     KDLLPGLQIEIRKDICNFLEIPEDELPYASELMQVKSRDSEWKGAIEKLLNNFGRSLI
                     VPDEHYSRVNAYINKTKLKGKLVYFRMFETDNKNIKPSSPKSLFYKLEFKPNSLFTHW
                     IETRILNDFNYICADSLKEFQEEDRAITKEGLIKHSRTRHEKDDRSSIHDKARYILGW
                     SNTDKVKALEEELKNFDNKYSDAYSKMDQLEKEKKAIHKTDLDLREILRFESFTELDI
                     ASIEKVIQETESHKKDLENSTGDLTKIQSKLTSIKSEIQKLEKEKSELEKKRNNLDRD
                     MEEADLEIKDCESVLKLIKDDEFEKHKSEIEKRVTGTNKLSLSNIKNKQNELHTLFEK
                     QREDREEIKQRTLTSIVRRMEAYIKNFEVDTTDLTAEIESISDFRKELSKIQNDKLPE
                     FQDKFRRMMDEKVAHQIAEFRENMVEQEKEIIEKIDELNEA"
BASE COUNT         1492 a          436 c          590 g          967 t
ORIGIN      
        1 agatgaatta atttctaata ttgattccct tctaaaatac aaacctaaag ttgaattaaa
       61 agaagttata gaaaaatatc cactgaaaaa aggtttagaa gaattagtta cttatttatg
      121 gatcgcatcg aatagagaac aacatttcgt tgattctaca aaaacagaag aatattcaat
      181 tcatattgat gatgaaaaaa cagtttcaat caaatttcca caggtggttt ataaaatcta
      241 atggaacata cacttcccta tgcagcagca gttttaaaat tactaaaagg tccagtaaac
      301 tatgaccttg atcctaaaga atgggaccaa cttgcaacct atcaaactga tataaaaaaa
      361 tactttgata agataggtct tcatttagtt gtagataatg atgatggttt cgcttatcta
      421 taccaacctt ccggcgatga tgatctagga ctaccaagga taacacgacg tatcccctta
      481 ccttttgata taacgttatt acttgtttta ctaagagaaa aattaactga acaagcaatc
      541 gatgatagaa atctggcaaa ccttttgaaa aaagaagatt tgtatgaatt actaaatcct
      601 ttctatccaa agactcaaaa cgaatctttg caatatagag aatacgatag aattatttca
      661 aaagtggtag aactgggttt tttaagaaag accgaaaaag ataaaaatga attttatcaa
      721 attgaaaaaa ttatcaaagt taggattcga gcagaagaat taaacacaat taaggcaagg
      781 cttttagaag acaatgacag aaagactgtt caagatgact gatatcaaca cagatatggg
      841 gtatcgccta ctccgtatgg aatttttaaa ttggggaact ttcagcaatg gtaaaatatt
      901 tgcagtggac acaaatggaa aaactgctct gataactggt gcaaacggat ccggtaaatc
      961 tacgttagtt gatgcactac tcacattact tgttccctca cagaaaagaa attataacct
     1021 tgccagtgga acagagagaa aacgtgatag ggatgagaaa acctatatcc aagggaatta
     1081 cggaaaaaca acagatgaga cgcaaggtag caaaatatta aagatgcgtg atccaagtga
     1141 gggacatata tcagttttac tcgcagtgtt ctatagtgaa gagattaagc gttatgcttc
     1201 tattagccag gttttatggt ttaacaatgg aaatctagaa aagttttatg tagtagcaga
     1261 aagagaatta aatataaaag atgattttaa acataaaggg gatattaaaa actttaaaaa
     1321 agtcctaaaa aatcaagaaa atataaaagt ttttgattcc tttaaagaat acattcatga
     1381 gtttattaaa aatgtagggc ttcgatctga aaaagcgtta gatttattta atcaaattgt
     1441 agcaattaaa tcaattggaa atctaaaaga attcatacga gaacatatgc tagaaaagca
     1501 tgatgtaaat gaactgattg aagatttgga taaaaattat aaaaatctaa acgatagtta
     1561 caatgcaata ttacaagcta gaagacagtt agaggaattg attcctattg aagaagaatc
     1621 tgttaaatac gaaaaaattg tagaagagat tcacaatata caaggaagta tttttatatt
     1681 acctgcgttc tttgctcggg agaaaatcaa tttattaaaa gaaaatctag aatcaacaaa
     1741 attcaatcta gagaaaatga atgatatagt tgaatccaat gaaaaggaaa tcataagaaa
     1801 aagagaggaa gaaagaagtc tttatgcaac tcttgaaaat gattcaattg gaagaagaat
     1861 tactgaacta caaaaacaat taatagatga gcgaaagatt cttgttggta gacaatctgc
     1921 ctatgataaa tataaaaacc tagctgcctc tttgttatta caaacaaagg ttacacaaga
     1981 aaatttctat gaaaataaaa agttagcaga agaaaaaatt cataaaaatt tagatctatt
     2041 aaaagaaatt gagaaaaaaa gagatcattg ggtcgttgtt cgaaatacat tgcacaatca
     2101 aaagcaagaa ttagaatctg aattaaattc actcaaaaca cgaaaagatt tattgcctgg
     2161 attacaaatc gaaatcagaa aagatatatg taattttcta gaaatcccag aagatgaact
     2221 tccttatgca agcgaactga tgcaagtcaa gtcaagagat tcagaatgga aaggtgcgat
     2281 tgaaaaatta ttaaataatt ttggtagaag cttaatcgtt ccagacgagc attattctag
     2341 agtgaatgct tatataaata agacaaagtt aaaagggaaa ttagtctatt ttagaatgtt
     2401 tgagacagat aataaaaata ttaaaccatc ttctcctaaa tcattgtttt ataaattgga
     2461 atttaaaccg aattcacttt ttactcattg gattgaaaca agaattctaa atgactttaa
     2521 ttatatctgt gctgattcac tgaaagaatt tcaagaagaa gatagagcta ttaccaaaga
     2581 aggattgatt aagcattcta gaacaagaca tgagaaagat gatagaagtt caattcacga
     2641 taaagcacgt tatatattag gatggtctaa tacagataaa gtaaaagctt tagaagaaga
     2701 attaaaaaac tttgataaca aatattctga tgcctattct aaaatggatc aattagaaaa
     2761 agaaaaaaaa gcaattcata aaacagattt agatttaaga gaaatcttac gttttgaaag
     2821 ttttacagaa ttagatattg catcgattga aaaagtaatc caagaaacgg aatcacataa
     2881 gaaagattta gaaaattcta ccggagatct tactaaaatc caatcaaagc taactagtat
     2941 taaatctgaa atccaaaaat tggaaaaaga aaaatctgaa ttagaaaaga aaagaaataa
     3001 tttagataga gatatggaag aagctgatct tgaaatcaaa gattgtgaaa gtgttttaaa
     3061 actaattaaa gatgatgaat ttgaaaagca caaatcagaa atagaaaaaa gagtaacagg
     3121 aacaaataaa cttagtttat ctaatataaa aaataaacaa aatgagcttc atactctttt
     3181 tgaaaagcaa agagaagata gagaagaaat taaacaaaga actcttacaa gtatcgttcg
     3241 aagaatggaa gcttacataa aaaactttga agtggatact actgatttaa cggcggaaat
     3301 tgaatccatt tcagatttta gaaaagaatt atctaagatt caaaatgata aactacctga
     3361 atttcaagat aaattcagaa ggatgatgga tgaaaaagtc gcacatcaaa ttgctgaatt
     3421 tagagaaaat atggtcgaac aggaaaagga aatcattgag aaaatcgatg aattaaatga
     3481 ggcat
//
LOCUS       DAOQOS010000251         3481 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_19171_length_3481_cov_6.589025, whole genome shotgun sequence.
ACCESSION   DAOQOS010000251 DAOQOS010000000
VERSION     DAOQOS010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3481)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3481)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3481
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19171_length_3481_cov_6.589025"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(21..518)
                     /locus_tag="PLF66_26110"
     CDS             complement(21..518)
                     /locus_tag="PLF66_26110"
                     /inference="COORDINATES: protein motif:HMM:NF015509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HMZ67590.1"
                     /translation="MQNIIILVICFFMGYFLRLRNRISRESYSVINQFILHISLPAQI
                     LIFINGITITKELLLPAGMAWILFLFVLSILYLFKKFFHLKNTAFACLLLTAGLGNTS
                     FLGIPMIETFFGKEFIWIGILCDQPGTFLILSLVAIPIAFYQVNIGSDRKLVINATLS
                     SRARL"
     gene            764..1540
                     /locus_tag="PLF66_26115"
     CDS             764..1540
                     /locus_tag="PLF66_26115"
                     /inference="COORDINATES: protein motif:HMM:NF024873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HMZ67591.1"
                     /translation="MINTSFLQFTGIGQQNIKKLHSLGITDWNAVLDQQANLPLSPKV
                     KEQILKEIPLYIDNYEKKDLKFFAERLHAKEKWKLLAEYFDQASYFDIETNGEPYGDN
                     ITLIVCYHKNRLYKFINGDNLDAFLDLLDDVTLLVSFNGNSFDVPIVENYFHIPKIPC
                     AHIDLRWLAYHIGFKGGLKSIEKEIGIQRPRELVGINGMDAILLWMAWKKDQSKEALD
                     LLIHYCCADVLSLQLLAGKILEKHKIDVHYPSPNEIWSRM"
     gene            complement(1756..2910)
                     /locus_tag="PLF66_26120"
     CDS             complement(1756..2910)
                     /locus_tag="PLF66_26120"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HMZ67592.1"
                     /translation="MFQFVIGAFVGILITSIVFLFWYRGYYLQFISRLDLIINSKEEH
                     LLEKVIEDRFLFEKYLKSEQQNRIQEDLSKKIIGYIDSLNQINFNLVQQDSTLDAMNA
                     FTENFSKVAESQSMSAENVAANTEELRASFESISQSVEKQVSSLSNVLIKLNSFIIFM
                     ETLSQEFSNLSSDSKKFTDRIHIGDKVVLSANEAMQNIAQSSNKIKEIAVNINEISEQ
                     TNLLALNASIEAARAGEKGLGFAVVAKEISKLSDRTVTSVKEIEIFVKNTSHDVKIGM
                     DTLKSTSEIIHEMLHWTEKLEEFLDRYAKAVLKKTSTISEVGNDLEFVKSESSGIKLA
                     SSEQTEAVHQISLKTQNVSNEAETILMSSIELGALVDELKRITNSLKNLS"
     gene            complement(2935..3345)
                     /locus_tag="PLF66_26125"
     CDS             complement(2935..3345)
                     /locus_tag="PLF66_26125"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HMZ67593.1"
                     /translation="MTDSYRKQHTELLEMATQLSTKLSVDIITKEANQIVTILSQFGS
                     KLNMHLTMEDKALYPKLINSGNAKTAQIANEYITEMGGIKQVVEKYLTSWSLAKNLIA
                     QPQKFIDESKGIITALKGRINKENNTLYPLADAL"
BASE COUNT         1182 a          615 c          506 g         1178 t
ORIGIN      
        1 atgaccactt caattctatt ttacaaacgt gcgcgacttg acaaagtcgc attaataact
       61 aacttacgat ctgatccgat attcacttga tagaatgcaa ttggaattgc cacaagagag
      121 agaattagaa aagttcctgg ttgatcacat aaaattccaa tccaaataaa ttctttccca
      181 aaaaaagttt caatcattgg aattcctaga aaagaagtat ttccaagtcc agcagtaaga
      241 agcaaacagg caaaggctgt attctttaaa tgaaaaaatt ttttgaaaag atataaaata
      301 ctcaaaacaa acaaaaataa aatccatgcc attccagcag gaagcaacag ttcttttgtt
      361 atcgtaatcc catttataaa aattaaaatt tgagccggaa gcgatatatg caatataaac
      421 tggtttatca cactatacga ctcccttgag atacgatttc ttaaccgtaa aaagtatccc
      481 ataaaaaaac aaataactag aattataata ttttgcataa ttccctttgc gcccttctta
      541 acgccagttc gatgtaatag aatatttaaa agattaccac cgataaaaga tacgataatt
      601 caaaatacat cgtttcttta tagttcagtt atcggtgcga tgcgctgagc gtagtcgaac
      661 cgtgcctctg tggtgaaaat atttttacat cgaactcacg tttcttaaat aaaaatattc
      721 ttttttcatt tcaaaaattc atttatagag taatttgaca agtatgatca atacttcttt
      781 tctacagttc acaggaattg gacaacagaa tattaagaaa ttgcattctc ttggtatcac
      841 agattggaat gcagtattag atcaacaagc aaatcttcct ctatcaccaa aagtcaaaga
      901 acagattcta aaagaaattc ctctctatat agacaattat gaaaagaaag atttaaagtt
      961 ttttgcagaa agactccatg caaaagaaaa atggaaacta cttgcagaat actttgacca
     1021 agcaagttat tttgatattg aaacaaatgg agagccctat ggagataata ttactttaat
     1081 cgtttgttat cacaaaaatc gtctctataa atttataaat ggagataatc tagatgcttt
     1141 tttggatttg ctcgatgatg ttacacttct agtttcattc aatggaaatt cctttgatgt
     1201 tcctattgtt gaaaattatt ttcatattcc aaaaattccc tgtgctcaca tcgacttacg
     1261 ctggttagcc tatcacattg gattcaaagg tggtctcaaa tcaatcgaga aagaaattgg
     1321 aatccaaaga ccaagagaac ttgtaggtat aaacggaatg gatgcgattt tactttggat
     1381 ggcttggaaa aaagatcaat caaaagaggc tcttgattta ctcattcatt actgttgtgc
     1441 agatgtgctc tctcttcaac tccttgcagg aaaaatatta gaaaagcata aaatagatgt
     1501 tcactatcca agtccaaatg aaatctggtc gagaatgtaa agaattcgtt taaaaaagaa
     1561 tcagtaaatc ttcctgatct atatggtatt aatttaaatt ccctactact ccgaaaaatg
     1621 cgttacacga tatattatat ttttaaaaaa agatgcatat cagatcgata gtcagtccaa
     1681 ctattctgcc acacaaggat tcggattaca gtctaaatat aaatatacgc ggatgaacag
     1741 gtaaaaataa taacatcaac ttaaattttt tagtgaattt gtaattctct ttaactcatc
     1801 cactaaagcc ccaagttcaa ttgaactcat aagaattgtt tctgcttcat ttgatacgtt
     1861 ttgagtcttc aaggatattt gatgcaccgc ctctgtttgc tctgatgatg ctagtttgat
     1921 tccagaagat tcacttttta caaattctaa atcatttccg acttccgaaa tagtagatgt
     1981 tttttttaat actgctttcg cataccgatc tagaaattct tccaattttt ctgtccagtg
     2041 cagcatttca tgtattattt cagaagtaga ttttaatgta tccattccaa ttttaacatc
     2101 atgacttgta tttttcacaa agatttcaat ctctttaacg ctagttactg ttctatcgga
     2161 tagttttgat atctcctttg ctacaaccgc aaagcctaaa cctttttccc cagctctcgc
     2221 tgcctcaata gatgcattca aagctagtaa gtttgtctgt tctgatattt catttatgtt
     2281 aactgcaatt tcctttatct tattggaaga ttgagcaata ttctgcatcg cttcatttgc
     2341 ggataatacc actttatctc caatatgaat cctatctgta aacttttttg agtcactgga
     2401 tagattagaa aactcctgac tcaatgtctc cataaaaatg ataaaactgt ttaacttaat
     2461 taatacattg gataaactgc ttacctgctt ttcaacagat tgactgatgc tctcaaaaga
     2521 agctcttaac tcttctgtat ttgcagcaac attttctgca gacatggatt gactttctgc
     2581 aaccttgcta aaattttcag taaacgcatt cattgcatcc agtgtagaat cttgttgaac
     2641 taaattgaaa tttatttgat tcagactatc aatgtaacca atgatttttt tagataaatc
     2701 ttcttgtatc ctattttgtt gctcactctt taaatacttc tcaaataaaa atctatcctc
     2761 tatcactttt tctagaagat gctcttcctt cgaatttata attagatcta acctagaaat
     2821 aaattgaagg taataaccgc gataccaaaa aagaaaaaca atcgatgtaa taagtattcc
     2881 gacaaaagca ccaattacaa attgaaacat tctcttaata gccagtcaaa tatttcaaag
     2941 agcatctgca agagggtaaa gcgtattatt ctctttatta attcttccct tcaaagcagt
     3001 tatgatcccc ttagattcat caatgaattt ctgaggctgc gcaattaaat tttttgccaa
     3061 tgaccatgat gttaaatact tttcaacaac ttgctttatt ccacccattt cggttatata
     3121 ttcgtttgcg atctgagctg ttttagcatt tcctgagttg attaacttag gataaagagc
     3181 tttatcttcc attgttaaat gcatatttaa cttactacca aattgagaaa gaattgtaac
     3241 tatttgattt gcttccttcg taatgatatc aactgataat ttggttgaaa gttgagtcgc
     3301 catttctaat agctcagtat gttgttttct atatgagtcg gtcattgcca tattgtagtc
     3361 ctcaaaatta ttctaatctt caatgagaaa gagtatcaaa ctgtatctca tgtagtatat
     3421 tagaatattc gtaacctatc agggtgatga aataaaaaat taaaggaagg aaacaaaggg
     3481 t
//
LOCUS       DAOQOS010000252         3354 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_20483_length_3354_cov_7.230070, whole genome shotgun sequence.
ACCESSION   DAOQOS010000252 DAOQOS010000000
VERSION     DAOQOS010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3354)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3354)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3354
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20483_length_3354_cov_7.230070"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            265..2358
                     /locus_tag="PLF66_26130"
     CDS             265..2358
                     /locus_tag="PLF66_26130"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HMZ67594.1"
                     /translation="MGIFNRACFVFLSLLSLTQVYSLEQILIDQKNSILSIGEYVEFY
                     EDKEQNIDFSSIKESKENWNESKRESLLFGFTQSKYWIRFRIKNNTTEKQLLVLDTNS
                     SRIDLIEFYNPDTKGVYRKIVTGDTFPYSQRDIDHYNFAFKIELEPEEEKTIFIAVSG
                     KYSVTNLTPKLLRLEFFYKESKEDILFLGIFYGFLFVMILYNLALFSRVKELAYLYLG
                     AFISFYLLYFLGFQGIGFQYLYSNSPWIQNRDFHISTPFIFLFSSLFANEFLDLKRNL
                     YKGYLANWFIVWAMLVWFVLNHLFVFNLYPTFSFFVASRSIVLGVFVALFNGVYLVFK
                     KNRSAYFYLGSWIVLLIANMIRFLEVTHIIPANQFTIYLVQIGLGSIVILLSFGLADK
                     MNLLKEELQKSKEDLEVKVLERTKELKDTLTEVNLLKEKQDGDYFLASLLVEPLAVNK
                     IETGTVLVDFFIKQKKEFYFKSQELRSIGGDICTAYSIKLRNRKYTTFLNGDAMGKSM
                     QGSSGALILGSLFGSIVNRNKFSPLNQDMTPEQWMRSAFLEMHRVFEVFDFSMLVSIA
                     FGLIENQTGTLFYINADHPQTVLYRDGRASFFEKNMRMPKLGTLGMTESISVQTFSLK
                     DGDILIAGSDGRDDLLDEKTGDMKWDESRFLNYVEKGKGNLEEIYELISLSGTIIDDL
                     SLLKVSFRLHADNKD"
     gene            2829..>3354
                     /locus_tag="PLF66_26135"
     rRNA            2829..>3354
                     /locus_tag="PLF66_26135"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         1100 a          484 c          694 g         1076 t
ORIGIN      
        1 tccaaacaat acggaaacaa tcgagtcgtt tcagaagagt taaataggcg ttatccaaaa
       61 gatgtagcga atgctattta ctatacaatg acttctgaaa attttgtaag aactgttttc
      121 tttttctcga tgggatacac tatagaattt taaaagtaat ctcttatcaa ttctctaaga
      181 aagcagatgg gatttgacac gtttcatttg atctaaactt ttttgtatca ttgacaaaat
      241 aaaggattca gttatccata aatcatggga atattcaata gagcctgctt tgtttttctc
      301 tctctcctga gtttaacgca agtctactct ttggagcaga ttctaataga tcaaaagaat
      361 tcgattcttt cgattggaga atatgttgag ttttacgaag ataaagagca gaatatcgat
      421 ttttcctcta taaaagaatc caaagaaaat tggaatgaat cgaagagaga gagtctactc
      481 tttggattta cgcaatcaaa gtattggatt cggtttcgta taaaaaataa tacaacagaa
      541 aaacaacttt tagttttaga taccaattcg agtagaatcg atttgatcga attttataat
      601 cctgatacaa agggagttta tagaaaaatt gtaacaggtg atacttttcc ttattcacaa
      661 agagatatcg atcattataa ttttgcattt aaaattgagc tagagccaga ggaagaaaaa
      721 acaattttta ttgcagtatc aggaaagtat agcgtaacca atttaactcc aaaactttta
      781 aggttagaat tcttttacaa agagtcaaaa gaagatattt tatttttggg tatcttttat
      841 ggattccttt ttgtaatgat cctttataat ttagcgttat tctccagagt aaaagaacta
      901 gcctatttat atttaggggc atttattagt ttttatcttt tatacttttt aggttttcag
      961 gggattggtt ttcagtattt gtattccaat tcgccttgga ttcaaaatcg ggattttcat
     1021 atttcaactc catttatatt tcttttttct tctttatttg cgaatgaatt tttagattta
     1081 aagaggaatt tatacaaagg atatctcgcc aactggttca ttgtatgggc aatgttagtt
     1141 tggtttgttt taaatcatct gtttgtattt aatctatacc cgaccttttc tttctttgtt
     1201 gcgagtcgct cgattgtctt gggtgttttt gtagctttat tcaatggagt ttatcttgta
     1261 ttcaaaaaaa atcgatctgc ttatttttat cttggttcat ggatcgttct actcattgca
     1321 aatatgattc ggtttttaga ggttacgcat ataataccgg caaatcaatt tacgatttat
     1381 ttagttcaaa ttggattggg ctcgattgta atattactct cttttggttt agcagataaa
     1441 atgaatttgt taaaggaaga attgcaaaaa tcaaaggaag atttagaagt aaaagtctta
     1501 gagagaacaa aggaattaaa agatacacta acagaagtaa atttattaaa ggaaaagcaa
     1561 gatggggatt actttttagc ttctctatta gtcgaaccgc tcgccgtaaa taaaattgag
     1621 acaggaactg ttctagtgga tttttttata aagcagaaaa aggaatttta tttcaaaagt
     1681 caggagctta gatcgattgg aggagatatt tgcacagcct acagtataaa acttagaaat
     1741 cgaaaatata caactttttt aaatggagat gcaatgggga aatctatgca aggttcgagc
     1801 ggtgcattga tcttaggttc tctctttggg tccattgtaa atagaaataa attctcccct
     1861 ttgaatcaag atatgacacc agagcaatgg atgcgatctg catttttaga aatgcatcgg
     1921 gtctttgaag tttttgattt tagtatgtta gtctcgattg cttttggctt gattgagaat
     1981 caaacaggaa ctctttttta tataaatgca gaccatccac aaacagtttt atatagagac
     2041 gggagagctt ctttctttga aaaaaatatg agaatgccta aattgggaac gttaggtatg
     2101 accgaatcga tttctgttca aaccttttct ttaaaggatg gagatatctt gattgcagga
     2161 agtgatggaa gagatgattt gctagatgag aaaacaggag atatgaaatg ggacgaatct
     2221 agatttctaa actatgtgga aaaaggaaaa ggaaatttag aagaaatcta tgaacttatt
     2281 tctttatctg gaacaattat tgacgatctt tcccttttaa aagtttcttt tcgactgcat
     2341 gcggataaca aagattaagc gctaatcaag attttcgatg aggcaaatca gtattatcct
     2401 ctatctgttt ttagcaatac aacgctttgg attaaagtct tttcgagtga tactctgccg
     2461 tgttatttcc ctacaagtag tgtgtgggtg tatcggaagt ggtgataaaa aggggtaaaa
     2521 aaaataaaaa aagttgggaa aaaagataaa aaagagtttg acgtgggagg tagtataaaa
     2581 tatgttgaaa agacggggca ggtagaagat cacttgctag cagagaagtt catttaaaaa
     2641 cagatatagt agcgtgaccc gattttgtag tcatacaaaa ttgttaaaaa atttgagaaa
     2701 caaaaatgat aaacgacagt ggatgtcaga tgtgtaaaag catttgatgt tttttgttag
     2761 gtgtatctct cttaggagag agcaaaacaa ttcatgaaat gataaaattt tcccgcaagg
     2821 gagaattcaa catggagagt ttgatcctgg ctcagaacta acgctggcgg cgcgtcttaa
     2881 acatgcaagt cgaacggatg tagcaataca ttagtggcga acgggtgagt aacacgtagg
     2941 taatctacct cttagttggg aacaacctgg tgaaaatcag gctaataccc gatagtattg
     3001 agttgtcgaa tggtgattca attaaaggat ttattcgcta agagatgagc ctgcggctga
     3061 ttagctagtt ggcggggtaa aggcccacca aggcgacgat cagtagccgg cctgagaggg
     3121 tgaacggcca cattgggact gagacacggc ccagactcct acgggaggca gcagttaaga
     3181 atcttgctca atgggggaaa ccctgaagca gcgacgccgc gtgaacgatg aaggtcttcg
     3241 gattgtaaag ttcaataagt gggaaaaaat aagctcgcaa gagtgatgat ggtacccatc
     3301 taaagcaccg gctaactacg tgccagcagc cgcggtaata cgtatggtgc aagc
//
LOCUS       DAOQOS010000253         3316 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_20869_length_3316_cov_4.023612, whole genome shotgun sequence.
ACCESSION   DAOQOS010000253 DAOQOS010000000
VERSION     DAOQOS010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3316)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3316)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3316
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20869_length_3316_cov_4.023612"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..426
                     /locus_tag="PLF66_26140"
     CDS             <1..426
                     /locus_tag="PLF66_26140"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HMZ67595.1"
                     /translation="VIAGKIGWEKESFLNELKKEMEEQKDIILLDNVNDVDLHNLYKS
                     SQIFLFASVYEGFGIPLLEALAHNKKCIVSNIPTFREIGKDKISYLETDSPKRWAEEI
                     QKLENTEMIPKINLEEFSWENSALITQNIFKKLLNNNEI"
     gene            416..1072
                     /locus_tag="PLF66_26145"
     CDS             416..1072
                     /locus_tag="PLF66_26145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010571570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HMZ67596.1"
                     /translation="MKFDELTTIKLWEIASRIPFESEYFIRIGRDKKLHVPDYFDPTI
                     KNFILELGSGWGEVGIELAKKHPNTGFILMEKNKDRLNVTVREIEKNKLKNIKLICCN
                     FNWFLSELFLENQFSQIILNFPDPWPKKKHWHNRTVNLEFIETLEKLLVSKGKFIFAT
                     DHGGYGRYAIRTFRKTKLFTPIGSEYVFFRKEFPISKFEKEKRSEDKTIYYLERERVN
                     "
     gene            1069..2103
                     /locus_tag="PLF66_26150"
     CDS             1069..2103
                     /locus_tag="PLF66_26150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010574026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HMZ67597.1"
                     /translation="MKFKTLLIGLGRIASLLEKDKFRYHPCTHAGVLFSPFGKKHFEL
                     SGIYDTDKEQTQQFLKQWNLNPKNLPYQISDLQNQYFDFAIIASSSEAHFENTQLAID
                     LGIRNLLIEKPICISKQQLKKLVHLKDKFALNIWVNHERRYHAFYRYVKDLIQTNQYG
                     ELRTIKASVLTSFRDPGNAFSGGGGPLLHDGTHAVDYIDYLLECEPIKVESKLIKANS
                     MSKIEEQAIALLTYPQNVFVFLEAGGKRKYFQFEIDIQTEEARFLLSNDGHHFYISKP
                     SKLYKGFRSLTEIPLPKIKKTESNPWINLYQEIVQVLSGKSSKIIGSLDANIRIYHTL
                     DTIYKKAKQS"
     gene            2249..>3316
                     /locus_tag="PLF66_26155"
     CDS             2249..>3316
                     /locus_tag="PLF66_26155"
                     /inference="COORDINATES: protein motif:HMM:NF015094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HMZ67598.1"
                     /translation="MNQFIDTSKNRTVFSYRNKGYISRFLLVYTFTLKRSLLFLWHYK
                     IRKRFSSKEQIEKKELELYTNLGRDAKTLFLNLGGVYIKLGQFLSNMGHILPDCFVSE
                     LKDLQDRIPPHSFEEIKLRFYKETNQNIETVFPTIDKTPIAAASTAQVYQAMLGEEKV
                     VVKILYPGIESLVEKDLKTVLFVMKRINRYLVTFDYKQIHSEVKTIITREMDLFEEAK
                     SIEKMAAYFSAEPSYVFPKVFHDYTTKGILVTKFIEGVKISETKSVTRKNSKKSRPLD
                     LLVRAYILMIFQYKFFHADPHSGNLIYTTDGKLCFIDFGAVSEVNDITAISLKKIISS
                     AMSKDYYGVVEGMEEMGFFDRD"
BASE COUNT         1240 a          539 c          540 g          997 t
ORIGIN      
        1 gtcattgcgg gaaagattgg ctgggaaaaa gaatctttct taaatgagtt aaaaaaggaa
       61 atggaagaac aaaaagatat tatattatta gataatgtaa acgatgtcga tttacataat
      121 ctatacaaga gttcccagat atttttattt gcctctgtct atgagggctt tggaattcca
      181 ctcctcgaag cactcgctca taataaaaaa tgcattgtat ccaacattcc cacatttaga
      241 gaaattggaa aagataagat ctcctattta gaaacggaca gccccaaaag atgggcagaa
      301 gaaattcaaa aactagaaaa tactgaaatg atcccaaaaa taaacttgga agaatttagt
      361 tgggaaaatt cagcacttat tacacaaaat atcttcaaaa aattattaaa caacaatgaa
      421 atttgatgag ctaacaacga ttaagctatg ggaaatagca tctcgtattc catttgaatc
      481 cgaatatttt attcgaattg gtagagataa gaaactccat gttcctgatt attttgatcc
      541 cacaattaaa aattttatat tagaacttgg ttcgggttgg ggggaagtag gaattgaact
      601 tgcgaaaaaa catccaaata cagggttcat tctaatggag aaaaataaag atcgattaaa
      661 tgtaactgtc agagaaattg aaaaaaataa acttaaaaat ataaaactca tctgttgcaa
      721 ttttaattgg tttttatcag agttattttt agaaaatcaa ttttcacaaa taatcctaaa
      781 ctttcctgat ccttggccca aaaagaaaca ttggcacaat cgaactgtaa atctagaatt
      841 tattgaaaca ttagaaaagc tccttgtaag taaaggcaaa ttcatatttg ccacagatca
      901 cggaggctat ggtagatatg ccattcgaac ctttagaaag acaaagctat ttacgcctat
      961 cggttccgaa tatgttttct ttcgaaaaga atttcctatc tctaaatttg aaaaagaaaa
     1021 gagatcagaa gataaaacaa tctactattt agaaagagaa agagtaaatt gaaatttaaa
     1081 acactcttaa taggtctagg tagaattgcc tctcttttag aaaaagataa attcagatac
     1141 catccgtgca cgcatgcagg cgtattattt tctccctttg gaaaaaaaca ttttgaatta
     1201 tctggaattt atgacacaga caaggagcaa actcaacagt ttttaaaaca atggaatcta
     1261 aatcctaaaa atcttccgta tcagatttca gatttgcaga accaatactt tgactttgca
     1321 atcattgcaa gtagttcaga agcacatttt gaaaatacgc aactagcaat cgatctagga
     1381 attcgaaacc tattgattga aaaaccaatc tgtatcagca agcaacaatt aaaaaagcta
     1441 gtccatctca aagataaatt tgcccttaat atttgggtaa accatgaaag aagataccat
     1501 gctttttacc gttatgtgaa agatcttatt caaacaaatc aatatggtga attaagaact
     1561 atcaaagcat ctgttcttac atcttttaga gatccgggga atgctttttc tggaggtgga
     1621 ggtccacttc tccatgatgg aacccatgca gttgattata tagattatct tcttgaatgc
     1681 gaaccgatca aagtagaaag taaattaatc aaagcaaact cgatgtcgaa aatcgaggaa
     1741 caagcaatcg ccttattgac ctatccccaa aatgtttttg tatttttaga agctgggggc
     1801 aaaagaaaat actttcaatt tgaaatagat atacaaaccg aagaggctag gtttttactc
     1861 agtaatgacg gtcatcattt ttatatcagt aaaccatcaa aattatacaa agggttccgt
     1921 agcttaacag aaataccttt gcccaaaata aaaaaaacgg aaagcaatcc atggatcaat
     1981 ctatatcaag aaatagtcca agttctgagt ggtaaatcct ctaagatcat tggatcttta
     2041 gatgcaaata ttagaatcta tcatacatta gatacgattt ataaaaaagc aaagcaatct
     2101 taagatctgt tttccttcat ttctaaaaga cagttttgac aaaattacta gagactagga
     2161 atttctttga aaaaaaacca attgttatga aaaaagataa tttcttgcat ttctgtgaat
     2221 atgcgaatga ctggtaatac tttccaaaat gaaccaattt atcgatacat cgaaaaaccg
     2281 aaccgtattt tcctatagga acaaggggta tatttcacga tttttactgg tttatacatt
     2341 taccctaaaa agatccttac tctttctttg gcattataaa atacgaaagc gtttttcctc
     2401 aaaagaacaa atagagaaaa aagaattaga actctatacg aatttaggac gagatgctaa
     2461 gactcttttt ttaaatttag ggggagtcta tattaagcta ggacaattct tatctaatat
     2521 gggtcatatt ttaccggatt gttttgtttc ggaattaaaa gacttacagg atagaattcc
     2581 accacattct tttgaagaga ttaagcttag attttataaa gaaaccaatc aaaatataga
     2641 aacagttttc cctaccatcg ataaaacccc tatagcagcg gcttccaccg cacaagtcta
     2701 ccaagcaatg ctcggtgaag aaaaagttgt agtaaaaatc ctttatcctg gaatcgaaag
     2761 tcttgtcgaa aaagatttaa aaacagtttt gtttgtaatg aaacgaatca atcgttatct
     2821 agttactttt gattacaaac aaatccacag tgaagtaaaa acaatcatca ctcgggagat
     2881 ggatttattc gaagaagcaa agtccatcga aaaaatggca gcttattttt ctgctgaacc
     2941 aagctatgtt ttccccaaag tatttcacga ctatacaaca aaaggcattt tagtaactaa
     3001 atttattgaa ggcgtaaaaa tctcagaaac aaaatccgtt acgcgaaaga actctaaaaa
     3061 atctagacca ctcgatttat tggtgcgtgc ttatatttta atgatctttc aatataagtt
     3121 ttttcatgca gatccccatt ccggcaattt aatttatact accgatggaa aactttgctt
     3181 tattgatttt ggagctgtca gtgaagtcaa tgatataaca gccatttccc ttaaaaagat
     3241 tatttcttct gctatgagca aagactatta tggagtcgtt gaaggaatgg aggaaatggg
     3301 attctttgat cgagat
//
LOCUS       DAOQOS010000254         3309 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_20946_length_3309_cov_6.025814, whole genome shotgun sequence.
ACCESSION   DAOQOS010000254 DAOQOS010000000
VERSION     DAOQOS010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3309)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3309)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3309
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20946_length_3309_cov_6.025814"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1129
                     /locus_tag="PLF66_26160"
     CDS             <1..1129
                     /locus_tag="PLF66_26160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67599.1"
                     /translation="DGSEEGIEVKMAEQSLQAIEEADVVLFMVDARAGVTSADIGIAN
                     HLRRQQKKVFLVANKTDGIDGDAHCADFYSLALGEIYQIAAAHGRGVTALLEKALSPF
                     LEELVEPTEPTLGPVERSHIDELPIQKMNFKNGQTVEYKVLLDKPEMVTLLFFLKKGE
                     EISGHYHLVNKAAYIYEGKAEVFAQNKLNPVQKGDTLYIPLNSVTSVKNTNDGITKVF
                     FFFPVGQFKTLAFNSPEEKNLDATGEKVQILKEVNVPWENWDGKIPAGEKVTPLAWKT
                     IIDNDSMVLGITKIDPNNDVDSHFHKPTQIVIFSDGEGKTHIAKGEYKEIKKDNYIYP
                     PTYSVHHSVNTSKTDPLMEIYFFPTGPFATIKYLGKGKKFW"
     gene            1183..1929
                     /locus_tag="PLF66_26165"
     CDS             1183..1929
                     /locus_tag="PLF66_26165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67600.1"
                     /translation="MKIKILIIISLMSLSLFAKSRGGVRMNISIPQFSAPIGGSSAPP
                     VILGPGLNPYYYNTPAYGNLILNQYNANINAINSRIPQPESDLGAQYLDKQIQKEIAE
                     DILTQPIQTGQSFPTYKITNINDHGKLDSNKDPKFKNIDYLLWKFQNIAEGNSIQMKG
                     NQLELGVSTVFVEKPESLLKFMQKEKDNFFLLEDRKEEVLVGYLVNRKTKKVNKVQCT
                     KVKDKAAIYDYALLGEELEFWNYIGLFGKE"
     gene            complement(2166..2774)
                     /locus_tag="PLF66_26170"
     CDS             complement(2166..2774)
                     /locus_tag="PLF66_26170"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016547187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HMZ67601.1"
                     /translation="MKVLLIDNYDSFTYNLYQYVGEILENLEKPFTFHVIRNDEKTIS
                     EIRNESYDRIILSPGPGDPKDREYFGVCADVLVDLGKTVPTLGVCLGMQGIAHYYGGK
                     VVRAFLPKHGKTSTIRHDGQGVFRNLPQDIEVMRYHSLVVDYESLPQCLTITAKIITK
                     EAGLEIMGLRHNEYPIEGIQFHPESFATEGGKKMLENFILSN"
     gene            complement(2784..>3309)
                     /locus_tag="PLF66_26175"
     CDS             complement(2784..>3309)
                     /locus_tag="PLF66_26175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002771084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HMZ67602.1"
                     /translation="IKLARTLLNDPKEIAEHNMLVDLHRNDLGRVAKYGTVKVRKLME
                     IKKYSHIQHISSEIIGIIKRGEDMFSGLASSFPAGTLSGAPKIESMKIIQRVENNPRG
                     PYGGALGHFGFNGNCTFAIPIRTLFVSGDYGYTQASGGIVFDSVPQSEYQEIKNKLGA
                     QEKVLNSFISRKIL"
BASE COUNT         1081 a          564 c          644 g         1020 t
ORIGIN      
        1 tgatggttcc gaagaaggta ttgaagtgaa aatggctgag caatcgctgc aagcgattga
       61 ggaggctgat gttgtgctgt tcatggttga tgccagagcc ggtgtaacca gtgccgatat
      121 tggtattgct aaccacttgc gtcgtcaaca gaagaaagtt tttctggttg cgaataaaac
      181 tgatggtatt gatggagatg ctcactgtgc agatttttat agtctggcat tgggtgagat
      241 ctaccagatt gccgcagctc atggacgagg tgttactgca ttgcttgaga aagcattatc
      301 accgtttctt gaagaattag tggagcctac tgagccaact ttaggtcctg ttgagagatc
      361 gcatatagat gagttgccaa ttcaaaaaat gaattttaag aatgggcaaa cggttgaata
      421 taaagtgctt ttagacaaac ccgaaatggt tacactttta ttcttcttaa aaaaaggaga
      481 agaaatatcg gggcattatc atttggtaaa taaagccgct tatatttatg aaggaaaggc
      541 ggaagtattt gcacaaaata aattgaatcc tgtccaaaaa ggggatactc tctatatacc
      601 gctcaatagc gtgacgtccg taaaaaatac aaatgatgga attacaaagg tatttttctt
      661 ttttcctgtt ggacaattca aaacgcttgc atttaattct cccgaagaga agaacttgga
      721 tgcaacggga gaaaaagttc aaatcttaaa agaagtaaat gttccttggg aaaattggga
      781 tggaaaaatt ccagcagggg agaaagtaac accacttgcc tggaaaacaa ttattgataa
      841 tgattcaatg gtgttaggaa taactaagat tgatcctaac aatgatgtag acagtcattt
      901 tcacaagcca actcaaattg taattttctc tgatggagaa gggaaaactc acatagcaaa
      961 aggggaatac aaagaaatta aaaaggataa ttatatttat ccgccaacgt atagtgtgca
     1021 ccattctgtt aatacaagta aaacagatcc tttaatggaa atctattttt ttccaacagg
     1081 tccttttgca acgatcaagt atcttggaaa aggaaagaaa ttctggtaga tataacttga
     1141 cgtttttcaa ttttcgtgta aggtgaaaga aagtagatca caatgaaaat aaaaatatta
     1201 atcatcatta gtttaatgag tctttccctt tttgctaaga gtaggggggg agttcgaatg
     1261 aatatttcca ttcctcagtt ttctgcaccg ataggtggaa gctctgcacc acctgtcatt
     1321 ttaggtccag gactaaatcc ctattattac aatacacctg cttatgggaa tttgatttta
     1381 aatcaataca atgcaaatat aaatgcaatt aacagtcgta taccgcaacc cgaatccgat
     1441 ttgggagccc aatatctaga taagcaaatt caaaaagaaa ttgcagaaga tattttaacg
     1501 caaccgattc aaacaggtca gtcttttcca acgtataaaa tcacaaatat aaacgatcat
     1561 gggaaattag attccaataa agatccaaaa tttaaaaata tagattatct tctctggaaa
     1621 tttcaaaata ttgcagaggg aaattcgatt cagatgaagg gaaatcaatt ggagttaggt
     1681 gtctctactg tttttgtaga aaagcctgaa tctcttctca aatttatgca aaaggaaaaa
     1741 gacaatttct ttctattgga agaccgcaaa gaggaagtat tggttgggta tttagtgaat
     1801 cgaaaaacaa aaaaagtgaa taaggttcaa tgcacaaagg tgaaagataa agcggctatt
     1861 tatgattacg cgttattagg agaagagtta gaattttgga attatatagg gctttttggg
     1921 aaagaataaa aaacctccta tagttacaga gaaccgtgct atgctccgag tgactaaacc
     1981 ggaaactaaa ggaggtcggt gatgctcttg gatttgcaac aggctctttc taaaaaaagc
     2041 gatgcatcca aaagaaagaa cctagagcag agtcacctca caagtcgttg taattttata
     2101 attcactaga ccacctcctt tcgtgtgctt acaatttata tagtagatca aaatagtcaa
     2161 gtggattagt tcgataaaat aaaattttcc agcatctttt ttccgccttc tgtggcaaag
     2221 gattctggat gaaactggat tccttcgatt ggatactcat tgtgtctaag tcccatgatt
     2281 tcaagtcctg cctcttttgt tataatcttg gcagtaattg tgaggcactg cggcaaagat
     2341 tcataatcca ctacgagtga atgatatcgc ataacttcta tatcttgcgg taggttccga
     2401 aatacgcctt gtccatcatg tcgaattgtg cttgttttac catgcttggg aagaaatgct
     2461 cttacaactt ttcctccata ataatgtgca atcccttgca tgcctaaaca aactccgagt
     2521 gttggaacag tctttcctaa atctacaaga acatccgcac agactccaaa gtattctctg
     2581 tcttttggat ctcctggacc tggagataaa ataattcgat cataggattc atttctaatt
     2641 tcagaaattg ttttttcgtc atttcgaatt acatggaatg taaaaggctt ttctaggttt
     2701 tctaaaattt cacctacata ttgatataaa ttgtaagtaa aggaatcata attgtctatt
     2761 agtaatactt tcatattttg ctcctataga atttttctag atataaaaga atttaaaact
     2821 ttttcctgag cacctaattt gtttttaatt tcctgatact ctgattgggg aactgaatca
     2881 aatactatac cgcctgacgc ttgtgtgtag ccataatcac cagaaacaaa aagagttcga
     2941 attggaattg caaatgtgca gtttccattg aatccaaaat gaccaagcgc tcccccgtaa
     3001 ggtccgcgag gattgttttc tactctctga atgattttca tggattcgat ctttggagca
     3061 ccagataacg ttcccgcagg aaaagaagat gctagaccgg agaacatatc ttctcctcgt
     3121 tttatgattc caatgatctc gctggatatg tgttggatat gactgtattt ttttatctcc
     3181 atcagttttc gcacttttac agttccatat ttagcaactc taccaagatc atttctatgt
     3241 aaatctacaa gcatgttatg ttctgcaatt tcttttggat cattgagtag agttcgagcg
     3301 agtttaatg
//
LOCUS       DAOQOS010000255         3288 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_21187_length_3288_cov_4.063718, whole genome shotgun sequence.
ACCESSION   DAOQOS010000255 DAOQOS010000000
VERSION     DAOQOS010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3288)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3288)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3288
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21187_length_3288_cov_4.063718"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(384..1301)
                     /locus_tag="PLF66_26180"
     CDS             complement(384..1301)
                     /locus_tag="PLF66_26180"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HMZ67603.1"
                     /translation="MDSLVITKPEQIGIYSLKLVGNITLDAHIKQFNLWAQENNVDSQ
                     GNSLVVSTSTIKDFFDHLLKDKNKYGRKNRASTLHGYKSAIKKSVSKTFKMTMEEKVA
                     FELFFRKIKLPKTTRQIKAMTIEELQALVDESDIRTSLMIRFLVISGLRISEMINIRQ
                     SDVMLSADKSDYNIYVVGKGNKEGLVKGIPKDLIQEIQTVFKGKEYLFERNKKPKRLN
                     ENKEEIMKFTRRQMYVLIEKAGLSILGKRINPHMTRHTCGRILMDRLKDVNKVQDRLR
                     HSSVRTTIENYLTTKITNKDVKEAFEGII"
     gene            1367..1711
                     /locus_tag="PLF66_26185"
     CDS             1367..1711
                     /locus_tag="PLF66_26185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020778644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67604.1"
                     /translation="MRVLFCLLLSFTVYAEQNPWGLSLFERILNRKHISHIVMESRPD
                     HFRVTLITSDRFPYNYKSVSYPFFIRFKEAAKAREVAKSIDEHLEKGYEMRIFLKGSE
                     IVSYKLLGNDSQ"
     gene            1788..3092
                     /locus_tag="PLF66_26190"
     CDS             1788..3092
                     /locus_tag="PLF66_26190"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HMZ67605.1"
                     /translation="MVSQSPIRTQEELESERRFSLFSFYTQWAVFCLFLIYKFDLNIF
                     VILSSLSFTAFRFLAILPSQKNSKRIYLLNHLASLSLICFFTFFFSAAIKNESNVFLD
                     WQLYYIALSFTVIHESMRVHRYSYLFLSGISLLIYTGIVFSVPELANLDITYKLIIPY
                     LFITYCASLGFFINRSRREAIRYYVDLLQEKIAMTKDMALARNVHESLFPKITTIKGL
                     KFTVFRKSHNQIGGDFYDFVQLREGNIGIFITDVAGHGYSSAMVAAMIKVMVSTLPYF
                     LKLDPSGLLSFLDKKISGEFNSHHATALYLFIDFQNKKFLLANAGHPYFIYAKKGEDF
                     KEIETFGTLLGFGLQDPVAATQEFNYESGDRFLIYTDGLIEGVNSKDELLESSGLLRI
                     LNKHKSQKSLNEFKDFVISDIHSFYGKTEFNDDAMLLAFEIE"
BASE COUNT         1060 a          560 c          486 g         1182 t
ORIGIN      
        1 attttttatt aaaatctatt ctgtaaaccc tttttcttat ttcgcaacag actgtctatc
       61 gatcgaacca agcctagcta aggctttgaa gttggataca aatggatttt tgaaaatgga
      121 ctctctctag gatttaaaat aagtcaactc aaaccttaaa atcattctct agatcaaaac
      181 aatcttctca ttgatacaac aaggcaaatc cttttataag attccatatt aaatggctag
      241 aatcaagcaa agtcggatgc aaaaaactct cttctttatt tctattgaaa atagttttga
      301 atcaaattgt atgaattcaa ttctactagg atgattttaa aataatacta tctgtttaat
      361 gtcaaaagaa atttcaacta tcgttagata attccttcaa aggcttcttt gacatcctta
      421 tttgtaattt tagttgtgag ataattttca atcgtagttc gaacggaaga atgtctgagt
      481 ctatcttgca ctttgtttac atctttcaat cgatccatta aaattctacc acaagtatga
      541 cgagtcatat gtggattaat tctttttcct aaaatagaaa gacctgcttt ttcaattaaa
      601 acatacatct gtcttcgagt aaatttcatg atttcctctt tgttttcatt taagcgctta
      661 ggttttttat ttctctcgaa taaatattct tttcctttga aaacagtttg aatttcctga
      721 atcaaatctt tcggaattcc ttttacaagt ccttctttat ttccctttcc tactacataa
      781 atattataat ccgatttatc agcagatagc attacatccg attgacgaat attaatcatc
      841 tcagaaattc taagacctga tataaccaag aagcgaatca tcaagctagt gcgaatgtct
      901 gattcatcga ctaacgcttg caattcttca attgtcattg ccttaatttg tcgagtggtt
      961 tttggaagtt ttatttttct aaaaaataat tcaaaggcaa ctttctcttc catcgtcatt
     1021 ttgaaagttt tagaaacaga cttttttata gcagatttat aaccatgcag agtggaagct
     1081 ctatttttcc taccatactt atttttatct tttagtaaat gatcaaaaaa atcctttata
     1141 gtacttgtcg atactacaag agaattacct tggctatcca cattattttc ctgtgcccaa
     1201 aggttaaatt gcttaatgtg cgcatctaga gttatattcc caactaattt cagagaatat
     1261 ataccgattt gttccggctt tgtaatgact agggaatcca tgctttcaaa tcgagaatcg
     1321 actttccttt ctcattcata aagttttttt tgtcgatagt tagaatgtga gagttctttt
     1381 ttgtttactt ctttctttta ctgtttatgc agagcagaac ccttgggggc tctccctttt
     1441 tgaaaggatc ttaaacagaa agcatataag ccatatcgtt atggaatcta gacccgatca
     1501 ctttagagta acattaataa catccgatcg atttccttat aattacaaat ctgtttccta
     1561 tccatttttt attcgattta aagaggcagc taaagcaaga gaggttgcaa aatcaataga
     1621 tgaacattta gaaaaaggct atgaaatgag gatttttttg aaaggctctg aaatcgtctc
     1681 ctacaaactg cttggaaatg attctcaatg agtatttaaa aagttttttt ataggcaaat
     1741 gcttgcaaga aacaaaggaa atgaaacatt gatcattaaa ctattttatg gtcagccagt
     1801 ctccaatccg cacacaagaa gaattagaat ctgagagaag atttagttta ttttcatttt
     1861 atacacaatg ggcagttttt tgtttattct taatttataa atttgatctc aacatttttg
     1921 ttattctatc tagtttatct tttactgctt tccgtttttt agcaattcta ccaagtcaaa
     1981 aaaattctaa acgaatctat ttactaaatc atttagctag cctctcccta atttgttttt
     2041 tcacattttt ttttagcgcc gctattaaaa atgaatctaa cgtctttcta gattggcagc
     2101 tttactatat tgctctatct ttcacagtca tacacgaatc aatgagagtg catagatatt
     2161 cctatctttt cttgagcggt atatctctat taatctacac tggtattgtt ttttccgttc
     2221 cagaattagc taatttagat atcacttata aattaataat tccatattta tttataacat
     2281 actgtgcctc gcttggtttt tttatcaatc gttccagaag agaggcaata cgttattatg
     2341 tagatttact ccaagaaaaa atcgcaatga cgaaagacat ggctcttgcg agaaatgttc
     2401 atgaatcgtt atttcctaag attacaacga tcaaaggatt gaagtttact gtatttcgaa
     2461 agtcgcacaa tcaaataggg ggagactttt atgactttgt gcaacttcgc gaaggcaaca
     2521 ttggaatctt tataaccgat gtggctggac atgggtattc ctctgcaatg gtagccgcaa
     2581 tgattaaagt catggtctct actctaccct actttttaaa attagatcca agcggtttac
     2641 tcagtttttt agataaaaaa atctcaggtg aatttaatag tcatcatgcg acagcactct
     2701 atctttttat agattttcaa aataaaaagt ttctactcgc gaatgctggt catccctatt
     2761 tcatctacgc aaaaaaagga gaggatttca aagagatcga aacctttgga actttactcg
     2821 gatttggtct gcaggatcca gttgcagcca cgcaagagtt taattatgaa tccggggatc
     2881 gttttttgat ttatacagat ggattaattg aaggtgttaa ctctaaagat gagttgcttg
     2941 aatcctctgg tcttttgaga atattaaata aacacaaatc tcagaaaagc ctaaatgaat
     3001 ttaaagactt tgttatttct gatatccact cattttatgg aaaaacagaa ttcaatgatg
     3061 atgccatgct acttgctttt gaaattgaat gaaaaaattg ataaaccaat ttaaatgatt
     3121 taaattcaaa atccgagttt aattttaaat ctgagcctcg gaggatcgac ttctacaatg
     3181 aatctaattt acaaattcta ctattctaca aaacatgaaa aacataagag gaaattatgg
     3241 aatttaaata tcttaaaatt aacaagcaaa acagaattgc aacaatag
//
LOCUS       DAOQOS010000256         3285 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_21215_length_3285_cov_5.113313, whole genome shotgun sequence.
ACCESSION   DAOQOS010000256 DAOQOS010000000
VERSION     DAOQOS010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3285)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3285)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3285
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21215_length_3285_cov_5.113313"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..256
                     /locus_tag="PLF66_26195"
     CDS             <1..256
                     /locus_tag="PLF66_26195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67606.1"
                     /translation="TDFSYSVFRANALKLNLSENRKYPNKTLFILGEESDYINEEDKD
                     LILKYFPNVKIETIPKAGHYLHYTHAKEFLEIVKNFILSL"
     gene            complement(240..1970)
                     /locus_tag="PLF66_26200"
     CDS             complement(240..1970)
                     /locus_tag="PLF66_26200"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HMZ67607.1"
                     /translation="MMIDILQHNKSSIRRTLIISFIIINLLTILFAGFTIYQMNGMHD
                     KNVKAANELLPAVIEISLLTGNIYDFRLTEFAHIATDSDKEMNAEEAKMESIKTKIEK
                     NFSVIDEIVKEEEALKIYREFKSEWKDYLKENSEILVISRKRLEKESDDKIKGSSLQK
                     FILAKETLAKLNNLTNKIAKDSSESSNVQVASTISVITFATLIITGIILFLSFSTIRN
                     IVNPLRTAMDVVTQLSKGDFTVEIDITKNDELGKMLFSIHDMVEHLSGSILQIRKLSG
                     EIAKSSAELGSVSANLNDSSQNMASSSEESSAAIEELTSSLDNVAHSIGIQARNMDEI
                     DANIKSMNTAILEIKMAADKLSHISSDSAKKATSGEAIANGTIIAMDKIKESSSKITE
                     IIGLISEISSQTNLLALNAAIEAARAGEAGKGFAVVADSITKLADRTVSGVKQIQNLI
                     YSTEKAISEGYNKVGEVAGILKGIIASVNNINNSVSGVITAVNKQVENARKIASNAEK
                     VTNLAKEIETASIEQKNGIGEINQSIVSVSETAQSVSAESANLRELSLSFKSKTDALE
                     KSVDFFKVKE"
     gene            complement(2158..2526)
                     /locus_tag="PLF66_26205"
     CDS             complement(2158..2526)
                     /locus_tag="PLF66_26205"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HMZ67608.1"
                     /translation="MREKESKVIVDSRFEGEVCLFSLIGELTLVDAPDVGQALDRIFE
                     KGFYKFLFDFDRLTFIDSSGISILIRFYGKLTKKEGKIAFCSFHKNVANAMQKALPLN
                     MRQTIYFEDRNSALNYLNFI"
     gene            complement(2529..3212)
                     /locus_tag="PLF66_26210"
     CDS             complement(2529..3212)
                     /locus_tag="PLF66_26210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67609.1"
                     /translation="MKSSLEQAIERGRRIVVVIYSFQPKMEKTNRLILSTILSQYKKT
                     DMHDSIYSVLFESIMNCIKANAKRIFFEECNLNIKNLEDYKSGISDFKEQIGNKRLRE
                     LCEKGRSKKIFIKVVYTHSQDGLKIEILNNAEILKEEEMRIRTKLSRGQKYENLFDFY
                     NDNSDPTEGEGLGLVLSLLMLKGEGVDPANFRIGSKDGVTKTRIEIPFNEYYKTERNL
                     YTLNLDSGL"
BASE COUNT         1007 a          576 c          517 g         1185 t
ORIGIN      
        1 gaccgatttt tcctattcgg tctttcgtgc taatgcatta aaattaaatt tatcagaaaa
       61 taggaaatat cctaacaaaa cactctttat cttgggagag gaatcagatt atatcaatga
      121 ggaggataaa gatttaattc taaaatactt tccaaatgtc aaaatagaga caattccaaa
      181 agcaggacat tatctacatt atactcatgc aaaagaattt ttagaaattg tgaaaaactt
      241 tattctttca ctttaaagaa atcaacactt ttctccagtg catctgtttt acttttaaag
      301 gaaagggaaa gttctcttag atttgcagat tcggctgaaa cagattgagc cgtttcggaa
      361 acgcttacaa tactttgatt gatttctcca attccatttt tttgttctat ggaagcggtt
      421 tcaatttctt tggcaagatt agttaccttt tctgcgttag acgctatctt tcgagcattt
      481 tctacttgtt tattaaccgc agtaatcaca ccgcttacag agttattgat attatttaca
      541 ctggcaatga tgccttttaa gatacctgca acttctccta ctttattata accttccgaa
      601 attgcttttt ctgtagaata aatcagattt tggatttgtt ttacacctga gacagttcta
      661 tctgcaagtt ttgtgatact atctgccaca acggcaaagc ctttaccagc ttctcctgcc
      721 cttgctgctt caatggctgc attgagagca agtagatttg tttgagagga aatttcagag
      781 atcagaccaa tgatttctgt aatcttggaa gaactttctt tgattttatc cattgcaatg
      841 atagttccat ttgcaattgc ttctccactg gttgccttct ttgcagaatc acttgaaatg
      901 tgagagagtt tatcagcagc cattttaatt tctaaaatag cagtgttcat ggattttata
      961 ttcgcatcaa tttcatccat attgcgagct tggattccaa tactatgggc aacattgtct
     1021 agggaggaag ttaactcttc aatagctgcg gaagactctt ccgatgaaga agccatattt
     1081 tgagaagaat catttaagtt tgcggaaacg gatcctagtt ctgcagaaga tttagcaatc
     1141 tctcccgata atttgcgaat ttgaagaatc gaaccactta gatgttcaac catatcatga
     1201 atgctaaaaa gcatttttcc taattcatcg tttttagtaa tatcaatctc tacagtaaaa
     1261 tctcctttgg aaagttgtgt caccacatcc attgcagttc tcagaggatt tacaatatta
     1321 cgaatcgtag aaaaacttaa gaatagaatt attccagtga tgatcaaagt agcaaaagtt
     1381 ataactgaaa ttgtagaagc gacttgaacg ttactagact cactcgaatc ctttgcgatt
     1441 ttatttgtga ggttatttaa tttagctagt gtttcttttg cgaggataaa tttttgaaga
     1501 gaagatcctt taattttatc gtctgattct ttctctaatc ttttacgaga aataactaat
     1561 atttcggagt tttcctttaa atagtctttc cattcagatt taaattctcg gtagattttt
     1621 aaagcttctt cttcttttac aatttcatct atgacggaaa aattcttttc aatttttgtt
     1681 ttgatgcttt ccatttttgc ttcttccgca ttcatttctt tatcagagtc tgttgcgatg
     1741 tgagcaaatt cagtcaatct aaaatcgtaa atgtttccag ttagaagact aatttctatc
     1801 acagcgggta atagctcatt agccgctttc acatttttat cgtgcatccc gttcatttga
     1861 tagattgtaa aaccggcaaa taagattgtt aaaagattta tgattataaa agaaataatt
     1921 aaagttcgtc gaatactgga tttattatgc tgtaaaatat ctatcatcat actgtttaaa
     1981 atatcggtat atgcctgtat tttgaggctt ttttttggat tttagataga gaaaccgtct
     2041 tttagaaagg gtttacctat cgaaaaagga atcaggatct ttcttttgta aacagagtga
     2101 tttaaagata agcttccata tgaaattatt ttagaaattt cgctcaagaa aaaagaacta
     2161 aataaaattt aaatagttca aagctgaatt tctatcctca aaataaatcg tctgacgcat
     2221 atttaaaggg agtgctttct gcattgcatt ggcaacattt ttatgaaagc tgcaaaatgc
     2281 aattttacct tcctttttag tcaatttgcc ataaaaacga attagtatgc taattcctga
     2341 ggaatcaata aacgttagtc ggtcaaaatc aaataagaat ttataaaatc ctttctcaaa
     2401 gattcggtct aaggcttgtc ccacatcggg agcatccact aaggttaact cgccaatcag
     2461 agaaaagagg catacctctc cctcaaatct agaatcaaca atcactttgg attctttttc
     2521 tctcataatc aaagaccaga atccagattt aaagtatata aatttcgttc tgttttataa
     2581 tactcattaa aagggatttc gattcgagtc ttggttactc catccttgct tccaatgcga
     2641 aagtttgcag gatcaacgcc ctctcctttt aacatgagaa gacttaaaac taaacctaaa
     2701 ccctctccct ctgttggatc tgaattatca ttatagaaat caaaaagatt ttcatatttt
     2761 tgacctcggg agagtttcgt tctgattcgc atctcttctt cttttaaaat ttctgcattg
     2821 tttaaaattt caattttaag accatcttgg ctgtgagtat agacaacttt aataaagatc
     2881 tttttggaac gacctttttc acaaagttct ctcagccttt tatttccaat ttgttcttta
     2941 aaatcactaa ttccagattt gtagtcttct aaattcttga tgtttagatt gcactcttca
     3001 aaaaagattc ttttggcatt tgctttgata caattcataa tggattcaaa taaaacacta
     3061 taaatagaat catgcatatc tgttttttta tattgggata ggatggtaga tagaatcaac
     3121 cgattggttt tttccatttt gggctgaaag gaatagatca ctacaacgat tcgccttcct
     3181 ctttcaatag cctgttctag agacgatttc atggattctt gatagccctg cttttgagag
     3241 tatatttcaa tctcgtcagg tcaattaatt tttcgatacc attgc
//
LOCUS       DAOQOS010000257         3274 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_21342_length_3274_cov_6.614787, whole genome shotgun sequence.
ACCESSION   DAOQOS010000257 DAOQOS010000000
VERSION     DAOQOS010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3274)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3274)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3274
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21342_length_3274_cov_6.614787"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..636
                     /locus_tag="PLF66_26215"
     CDS             <1..636
                     /locus_tag="PLF66_26215"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF018649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HMZ67610.1"
                     /translation="LFVTMSLMLFLPAWTTDYLLAWIYLSIFFSGIFFITIYIFIFDP
                     KLLESRLKVGPVSEQRPIQKAIQSFASIGFLSVYIISSFDYRFHWSNVPSLVSYLANG
                     ILAFTMYLFFLVFKINSFLSATVEIQENQKLITDGVYSIVRHPMYFAASLLFLTTPIA
                     LGSYYGLFSLPFMLLVLMYRSIDEEKMLIKQFPEYIEYSKKVKKRILPYIW"
     gene            1428..2030
                     /locus_tag="PLF66_26220"
     CDS             1428..2030
                     /locus_tag="PLF66_26220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67611.1"
                     /translation="MYRNIKIGLLIKEGGLKEYILSALVDAHYLIIEKKWDDNFVDSD
                     LETFDTLVLDISQFKEKDFHLLKKIKNLFPDLKILILSGSNADESIYKAICFGANGYI
                     CKSEIRNLEKIIEMILNRESCISSNIALEIYQYFLSIYRKNLSDILSISEIAVLENSI
                     SGISIANVASKLNLPVYSIQKIIYSIYLKLQTIKHFDLTE"
     gene            2253..3059
                     /locus_tag="PLF66_26225"
     CDS             2253..3059
                     /locus_tag="PLF66_26225"
                     /inference="COORDINATES: protein motif:HMM:NF018387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 family protein"
                     /protein_id="HMZ67612.1"
                     /translation="MSSDKLMIPKTNSIQIIEQEDIENTTKLYFKTTKNSGKVLFTTK
                     NGLKLEIIIYSPISDADEDGFPDAVELSHEDEIRFRNWFVRIAEAQFIKPNSFWNPNE
                     RDCAGLIRYSFREAFKKHDENWYKQSGFSLDKNLPDITSYHYPDIPILGIRIFKQKKG
                     DSLDLESFGTFANAETLFRWNTVFISKNIVESKKGDLLFFENRGEIHFPFHSMIVASN
                     DGREPIIIYHTGEKDILKRVPVSYLENSKIFKVSPENPHFLGVFRFSILE"
     gene            3063..>3274
                     /locus_tag="PLF66_26230"
     CDS             3063..>3274
                     /locus_tag="PLF66_26230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67613.1"
                     /translation="MINRIFQIIIFLSFCLFPFVSFADENSTPEFYLSTDKSFSKEEM
                     PYVNLEGPGNLNYSIRVYEIADPEKFL"
BASE COUNT         1094 a          506 c          482 g         1192 t
ORIGIN      
        1 ttattcgtaa ctatgtcttt aatgctattc cttcctgcat ggacaactga ttatcttctc
       61 gcatggattt atctaagtat atttttcagt ggaatatttt ttattactat ttatattttt
      121 atttttgacc ccaaacttct ggagagtaga ttaaaagttg gtcctgtttc agaacaaaga
      181 ccaattcaaa aggcaatcca aagctttgca tccattggat ttttatcggt ctatatcatt
      241 tctagtttcg actatcgctt tcactggtca aatgttccaa gtttggtttc ctaccttgca
      301 aatggaattc tagctttcac tatgtatctc tttttcttag tattcaaaat aaattccttt
      361 ttaagtgcaa ctgttgaaat ccaagaaaac caaaagctaa ttacggatgg agtctattca
      421 atcgttagac atccaatgta ttttgcagcg agtttgctat ttttgacaac gcctatcgct
      481 ttaggctctt actatggtct tttttctctt ccttttatgc ttttagtttt aatgtataga
      541 tcgattgatg aagagaaaat gttaataaaa caattccctg aatatataga gtattctaaa
      601 aaagtaaaaa aacgtattct gccctatatc tggtaacatt ctttctttaa tttaaaaatt
      661 ttattcaaag aattcagttt ttaaatcctt ctctgtttat taattcgttc ctattcttca
      721 atcaattact aactcacctt acattatcat tgcttaacaa aaacaatgat tgaatttggg
      781 tgagcctaac gttcaaatga agtatgcgcc tagcgacggg ccttcctccc atcgcttttc
      841 gctccacatc agccgagcta taccggaggc tattaaattt actcatttat tagcttcctc
      901 taatattcta atttccttta tcatacttat caatttctat cttttgtgta aggtcagtta
      961 ctaactaatt ttacacaaat ttaagagttt tcagtaaatc ataattttat gaatattaga
     1021 aaatacgaag gaaagaatta tatctatatg agtggacaag tccttctatt catctcattc
     1081 tcactcctag agcctattat tatttttcaa taatgatatc atttaaacat ttggttctcc
     1141 ttgcataagg tgaattacga agcgatagct gatttctatc aaagagattt tatatttgta
     1201 tttttagaaa ttttttaata cgatttaata cagcattcta ttctttccat ttttttggtt
     1261 agtttactct ttctagtttc aaatacaaat taagaaacag actcagtctt tattaaaacc
     1321 taacaaaatt gatagtttta tcaagctttc atagaattgt tattttcagg gagagcgaaa
     1381 taagatatta taaaagaaat tggtcattta aagataatga gaattgtatg tatcgaaata
     1441 taaaaatagg attgttaata aaagaaggcg gactgaaaga atacattcta tcagcgttag
     1501 tcgatgcaca ctatctcatt atagagaaga aatgggatga taatttcgtc gatagtgacc
     1561 tcgaaacatt cgatactttg gttctagaca taagtcaatt taaagaaaag gatttccatt
     1621 tattaaaaaa gataaaaaat ttatttccag acttaaaaat attaatttta tcgggaagca
     1681 atgcagacga atctatttat aaagcaattt gctttggagc aaatggctat atttgtaaaa
     1741 gtgaaataag aaacttagag aaaataattg aaatgatcct aaatagagag tcttgcattt
     1801 cttccaatat cgctttagag atatatcaat actttttatc tatttataga aaaaatcttt
     1861 ctgacatttt aagcatctca gaaattgctg tattagaaaa ttcaatcagc ggaattagca
     1921 ttgcgaatgt agcttccaaa ctaaatctgc ctgtctattc tattcaaaaa attatctatt
     1981 ctatctattt aaaattgcaa actattaaac attttgattt gaccgaatag taagaagaga
     2041 aatctttttt aaaataaacc attctcttag attattttat gataaaacag aaattatatt
     2101 ctgctaggct tattgcattc ttatatttca tttttttatt catctgtttt tgtttcttca
     2161 ataaatgcaa acaagactct ctctattcct cattaacgtc ttatcctgcc gatggagtga
     2221 gcggaggtat tttaatattt gaaaaatctt ttatgagttc agataaatta atgattccaa
     2281 aaacaaattc tattcagatt atagagcaag aagatattga gaacactacc aagctctatt
     2341 tcaaaacaac taaaaactcc ggcaaagtat tgtttaccac aaaaaatgga ttaaaattgg
     2401 agataattat ttactctccc atttcagatg cagatgaaga tggctttccc gatgcggttg
     2461 aactttctca tgaagatgag attcgattca gaaactggtt tgtaagaatt gcagaagctc
     2521 agtttataaa accaaatagt ttttggaacc caaatgagag ggattgtgca ggactcattc
     2581 gttactcatt tagagaagca ttcaagaagc atgatgagaa ttggtataaa caaagtggat
     2641 tttctctcga taaaaattta cccgatatta ctagttatca ttatccagat atacccattt
     2701 taggaattcg tatttttaaa caaaagaagg gagactctct agatctggaa agttttggaa
     2761 cctttgccaa tgcagagaca ctttttcgat ggaatactgt atttatttct aaaaatattg
     2821 tagaatctaa gaagggtgat ttgttattct ttgaaaatag aggagaaatt cattttccat
     2881 ttcactccat gatagtggca tcaaatgatg gaagggaacc gataattatt taccatacgg
     2941 gcgaaaaaga tattttaaaa agagttccgg tttcttatct tgagaatagt aaaattttca
     3001 aagtctcacc ggaaaatcct cattttctag gtgtttttag attttcaata ttggagtaaa
     3061 tcatgattaa ccgcattttt caaattatta tatttctcag tttttgttta tttcctttcg
     3121 tatcctttgc agatgagaat tctactccag aattttactt aagcactgat aaaagttttt
     3181 ctaaagaaga aatgccctat gttaatttag aaggtccagg aaatctaaat tattcaattc
     3241 gtgtttacga aattgcggat ccagaaaagt ttct
//
LOCUS       DAOQOS010000258         3263 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_21469_length_3263_cov_6.051434, whole genome shotgun sequence.
ACCESSION   DAOQOS010000258 DAOQOS010000000
VERSION     DAOQOS010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3263)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3263)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3263
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21469_length_3263_cov_6.051434"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2547)
                     /locus_tag="PLF66_26235"
     CDS             complement(<1..2547)
                     /locus_tag="PLF66_26235"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004788861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HMZ67614.1"
                     /translation="MNINRYFTQGSESLYPNIEWASRNSKISNSDGSSVFEANDIKVP
                     SGWSQVATDILAQKYFRRRGIPKYLKKVEEPGIPEWLQRSIPDQEKLNSLNPEDRYYG
                     ENDARQVFHRLAGCWTYWGYKYGYFSNEESARVFYEETIYMLASQMAAPNSPQWFNTG
                     LHWAYGIDGASQGHYYVDPKTGVLTKSASSYEHPQPHACFIQSVNDDLVNEGGIMDLW
                     VREARLFKYGSGTGTNFSNIRGENEALSGGGKSSGLMSFLKIGDRAAGAIKSGGTTRR
                     AAKMVCLDMDHPDIEEFIEWKVEEEKKVASLVTGSIHNNRALNKVIKACHEFETANES
                     DKFDPTKNPNLKKAIIEARKSFCPDNYIKRVIDLAKQGAKEILFEELTTDWQSEAYNT
                     VSGQNSNNSVRISNDFMEAVEKDYDWNLFNRTELDKAKKLGREPKAAKTIRARDLWEK
                     IANAAWASADPGTQYHTTINEWHTCPEDGAINASNPCSEYMFLDNTACNLASANLQKF
                     LKADGSFDVDAFRYLCHIWTIILEISVTMAQFPSKEIAELSYRFRTLGLGYANLGSIL
                     MIMGIPYDSKEALGVTGAITSIMHMTAYKTSALMAQELGPFAGYEKNKEHMLRVMRNH
                     RRAAYNSPSEDYEGLTIKPMGIDSANCPAYLLNAAKEDSDAALELGQKYGYRNAQVTV
                     IAPTGTIGLVMDCDTTGIEPDFALVKYKKLAGGGYFKIINQSVPPALKKLGYSPSEIE
                     AIVNYCKGYATFNGAPYINTQTLKAKGFTDEVLEKIERDLAAAFDINFVFNKYILGEE
                     FLSKTLKLTKEVYDAPNFNLLEHLGFSKDEIRKANDYVCGTMTVENAPFLK"
     gene            2739..2811
                     /locus_tag="PLF66_26240"
     tRNA            2739..2811
                     /locus_tag="PLF66_26240"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2772..2774,aa:Asn,seq:gtt)
BASE COUNT          974 a          627 c          594 g         1068 t
ORIGIN      
        1 ctttaagaac ggagcattct ctactgtcat ggttccgcaa acataatcat ttgcctttcg
       61 aatttcgtct tttgaaaacc caagatgttc tagtaaatta aagttaggcg catcataaac
      121 ttctttagtt agtttcaaag tctttgataa aaactcttcc cctaaaatgt atttgttaaa
      181 aacgaaattt atatcaaaag ctgcagctaa atccctttca attttttcta gaacttcatc
      241 tgtaaagcct tttgctttta aggtttgtgt attgatataa ggagcaccgt taaatgtagc
      301 gtatccttta caataattta cgatcgcttc tatctcagaa ggagaataac ctagtttttt
      361 aagagctggt ggaacagatt ggttgatgat tttaaagtat ccaccacctg ctaatttttt
      421 atatttcaca agagcgaaat ctggttcaat cccagtagta tcgcaatcca taacgagacc
      481 gatagttcct gttggtgcaa ttacagtcac ttgggcattt ctatatccat atttttgtcc
      541 taattctaaa gcagcgtctg aatcttcttt tgcagcattt aaaaggtaag caggacaatt
      601 agcagaatca attcccattg gtttaattgt taaaccttca taatcctcgg aaggagaatt
      661 ataagcagct cttctatgat tgcgcataac acgaagcatg tgctctttat ttttctcata
      721 gccagcaaaa ggtccaagct cttgtgccat aagtgcagag gtcttataag cagtcatgtg
      781 catgatagaa gtaattgcac ctgtaactcc taacgcttct tttgaatcat aaggaattcc
      841 cataatcatt aaaatagatc caaggtttgc atagcctaaa cctaaggttc taaatctata
      901 agaaagttct gcaatttctt tagatggaaa ttgtgccatt gtaacagaaa tttcaagaat
      961 gatagtccag atatggcaga gataacggaa tgcatctaca tcaaaacttc catctgcttt
     1021 taagaatttt tgtaaatttg cagaagctag attacatgca gtattatcta ggaacatata
     1081 ctcagagcaa ggattagatg cgttaatcgc tccatcctca ggacaagtat gccattcatt
     1141 gattgtagta tgatattgtg ttcctggatc agcagaagcc caagccgcgt ttgcaatttt
     1201 ctcccaaaga tctcttgctc ggattgtctt tgctgctttt ggctctctac ctaacttttt
     1261 agctttgtct aattctgttc tgttaaataa attccagtca tagtcttttt caactgcttc
     1321 catgaagtcg ttagaaatac gaacagagtt attgctattt tgaccagaaa cagtattgta
     1381 agcttctgat tgccaatctg tcgttagttc ttcaaacaga atttcttttg caccttgttt
     1441 ggcaagatca atgacacgtt tgatataatt gtctgggcaa aaagacttcc ttgcttcaat
     1501 gattgctttt ttcaaattag gatttttagt tggatcaaat ttatcggatt catttgcagt
     1561 ttcaaattca tgacaagctt tgattacttt attaagagca cggttattgt gaatagaacc
     1621 ggttacaaga gaagctactt tcttttcttc ttccactttc cattcaatga attcttcgat
     1681 atcagggtga tccatatcga gacaaaccat tttagcagca cgtcgagtcg ttccgccaga
     1741 tttaattgca cctgccgcac gatctccaat ttttaagaaa ctcatgagac cagaactctt
     1801 tcctccgccg gataacgctt cattttctcc tcggatgttt gaaaaattcg ttcctgttcc
     1861 agaaccatat ttaaaaaggc gagcttctct gacccagagg tccatgatcc cgccttcatt
     1921 gactagatca tcatttacac tttgaataaa acaagcatga ggttgtgggt gttcatacga
     1981 agaagcggat tttgtcagaa cgccagtttt tggatctaca tagtaatgac cttgggaagc
     2041 tccatcaatt ccatatgccc agtggagacc tgtattaaac cactgcggag aattgggggc
     2101 agccatttga gaggcgagca tataaatcgt ttcttcgtag aaaactcgtg cactctcttc
     2161 attggagaaa tagccgtatt tataacccca ataggtccaa cagccagcga gtctatgaaa
     2221 tacctgacgg gcatcgttct ccccgtaata tctatcttca ggattgagag aattgagctt
     2281 ctcttggtct ggaatggagc gttggagcca ttcaggaatt ccaggttctt ctactttttt
     2341 taaatatttt ggaattccgc ggcgacggaa gtatttttgg gcaaggatat cggtggcaac
     2401 ttgggaccaa ccagaaggaa ctttgatatc atttgcttca aaaacagaag agccatccga
     2461 attactaatc tttgaatttc tagacgccca ttcgatgttg gggtaaagag actcagagcc
     2521 ctgtgtaaaa taacgattta tattcataga taaatatgtc tccaaatagg aatagatgtg
     2581 ttatgtcaca ctgcataatt caaaaaaaat atctgtcatt ttttttccaa agctatgttt
     2641 tttttccatt tttttagatt aaaaaatcta tgttattcac atttttctag ttgagctttt
     2701 tgactctctt ggaatcatgg catcaagact gattacaatc ccctttagct cagtcggtag
     2761 agcaaatgac tgttaatcat tgggtcgctg gttcgagccc agcaggggga gcgaagttcc
     2821 gttagccatc aggcggtagg aactgaggct tgctagaccg cttcatcaaa gcggggggcg
     2881 gcattctact ataaattata acttaaaact aaatatttgt tagccttcgg cggaaggaaa
     2941 aattgctaaa ggcttttgcc catcaggcaa aagtaaggac ttctagtata aattacccct
     3001 taaaactgaa tccctacaaa attaaaagat tgccaccgat aaaagatacg ataattcaaa
     3061 attatttatc gttcagttat cggtgacatc ggtggtgaaa aaatttttac attaaattca
     3121 cattaaacaa tttgagtttc aaatatttac aaatcaattt gtcttatagt tttgcacata
     3181 cagatcgatc aggttcttga tcattttcca gtctgttgcg aaatgttaaa gaattaaaag
     3241 tcagccttta aaaataaatg aat
//
LOCUS       DAOQOS010000259         3247 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_21628_length_3247_cov_6.033521, whole genome shotgun sequence.
ACCESSION   DAOQOS010000259 DAOQOS010000000
VERSION     DAOQOS010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3247
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21628_length_3247_cov_6.033521"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(53..385)
                     /locus_tag="PLF66_26245"
     CDS             complement(53..385)
                     /locus_tag="PLF66_26245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67615.1"
                     /translation="MKKIYWFLFLFVALVFQLFSADASLFVLAVGIPVLNSFDSSGPL
                     NAGVISEHKNSERITSWAITTTIKFGRAISEQSAGFAKLIGNATDRFIGVAGFSTAAG
                     DIDNQQYL"
     gene            complement(394..1599)
                     /locus_tag="PLF66_26250"
     CDS             complement(394..1599)
                     /locus_tag="PLF66_26250"
                     /inference="COORDINATES: protein motif:HMM:NF021494.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2213 domain-containing protein"
                     /protein_id="HMZ67616.1"
                     /translation="MAEKNLRYDNGSIEPLEIGEGFVRARVLFARDGVFPYVYPDGSI
                     KMEAKLDSELLSSSTIESAKGKPICDGHPPLSDSQGLITPENYNRYVKGALGDSVSVI
                     NRDGINYLSAYETIFDPSLIESLKANEKLEVSIGFESDIDFTSGEYRGQRYDAVQRNI
                     KINHIAHVEKGRAGDSVRAYLDSGINRIAVMQSQNIETQKENKENVMVKTPNIPRTKQ
                     DWKDFLKKIFKTDSSEELSEAADAIEAATEAVQTPEDANPQPADVKKSEDLIASLQNQ
                     ITALQQVLEAKQKELESLMSPATQDALVTRRLSLVEIARSIDPAFKQDGLSNREIKLA
                     VIGKTLPFPNADVSKLDESLIDARFDAAVELAREKASLRTDSKVDTVRIDESSIEKKR
                     EERKNLWGK"
     gene            complement(1664..2488)
                     /locus_tag="PLF66_26255"
     CDS             complement(1664..2488)
                     /locus_tag="PLF66_26255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67617.1"
                     /translation="MEKELDVFQAVDGRIQFEDYGKENGMTYWFARDLMTYLEYEDFS
                     AFRIVISKAMTTCHTLNIDTIDDFRQVEREIDGKKVTDFKLSRFACFLISMNADPKKK
                     NVAKAQIYFTKIASLFEDSKELSKSVERLLVRDEISQKEKVISGLAHAQGVMNYPLFQ
                     NAGYRGLYNKNLKDLKEYKGMEDFNKSLLDFMGSEELAANLFRITQTTLKIKNENIKG
                     QRNLENVAETVGHKVRKSMIEISGIKPEDLPLTSDIKETKKVLKQANQKFKKIKKK"
     gene            complement(2552..2725)
                     /locus_tag="PLF66_26260"
     CDS             complement(2552..2725)
                     /locus_tag="PLF66_26260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67618.1"
                     /translation="MTEDEKKVLEKKSYSIDCTEDDFILYWTNVKGRSVEEAKEAYPI
                     IMNEIARAHFNIK"
     gene            complement(2729..>3247)
                     /locus_tag="PLF66_26265"
     CDS             complement(2729..>3247)
                     /locus_tag="PLF66_26265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67619.1"
                     /translation="INMDKVYEQSPIQFQAMSETNPLKSMASGVYDNGVITLNESSAN
                     AMLTVVHETAHHLEAKVLGNGFSFLRTPEGQSLLSEIKNTTTFKNLLANQNSIPSELY
                     SYYTQDSEIFTRIMEQYFANKQGGILKKELLSKANIIHIQSGVDLYLSNQEFKKIEIL
                     IDQIFKSKGLMR"
BASE COUNT          971 a          649 c          525 g         1102 t
ORIGIN      
        1 acaatatgag aagcaatttg cctaaaatcg tgtccactaa atatgtagta gttcatagat
       61 attgttgatt atcaatatcc cctgccgcag tagaaaaacc agccacacct ataaaacgat
      121 ccgtagcatt tccaatgagt ttagcgaatc cagcagattg ttcagagata gctcttccaa
      181 atttaatagt agtagtaata gcccagctag tgatgcgctc tgagttttta tgttcagaga
      241 taacacctgc attcaaagga ccgctagagt cgaaactgtt tagcactgga attccgacag
      301 cgagaacaaa aagcgaagcg tcagccgaaa aaagctgaaa cacaagcgca acgaataaaa
      361 acaaaaacca ataaatcttt ttcataaagc ctcctatttg ccccaaagat tttttctttc
      421 ttctcttttc ttttcgatgg aagattcatc gattcgaaca gtatccactt tggaatcagt
      481 tcgaaggctt gctttttctc ttgcgagttc tacagccgca tcaaaacgag catcaatcag
      541 agactcatct agcttagaaa catctgcatt tggaaaaggc aatgtcttac cgataaccgc
      601 aagcttgatc tctctgttag agagtccatc ttgtttgaaa gctggatcaa tcgatctagc
      661 aatttcgaca agcgataggc ggcgagtaac gagagcatct tgagtcgctg gactcataag
      721 agattctagc tctttttgtt ttgcttctaa tacttgttgt aatgcagtga tttgattttg
      781 cagagatgcg ataagatctt ctgatttctt cacatcagca ggttgagggt tagcatcctc
      841 aggggtttga actgcttccg tagcggcttc aatggcatcg gcagcttctg aaagctcttc
      901 cgaactatca gtcttgaaaa tctttttcaa gaaatctttc cagtcctgtt ttgtccttgg
      961 tatatttggt gttttcacca taacattctc cttattctct ttttgtgtct ctatgttttg
     1021 cgattgcata acggcaattc gattgatgcc actatccaaa taagcgcgga cagaatcacc
     1081 cgcgcgacct ttctctacat gtgcgatatg gttaatttta atattgcgtt ggacggcatc
     1141 atatcgttgt ccacgatatt cgccagatgt aaaatcaatg tcagactcaa agccaatcga
     1201 tacttctaac ttctcgtttg cttttaaact ctcaataaga gatggatcga aaatagtttc
     1261 gtaagcagat agataattaa ttccatcccg attaattaca gaaacggaat caccgagagc
     1321 ccctttcaca tatcgattgt aattttcagg agtgataaga ccttgagagt cggataacgg
     1381 aggatgcccg tcacagattg gtttgccttt agcagattca attgtagagg aagacaaaag
     1441 ctcagagtca agcttcgctt ccattttaat acttccatcg ggatagacat aagggaaaac
     1501 cccatcgcgt gcaaatagaa ctctcgcacg gacaaagcct tctccaattt caagcggttc
     1561 aatcgaacca ttatcataac gtagattttt ttctgccata cagattgtat cgcagaggat
     1621 aagattattt tgtcagatac tccccctttt acaaagggaa tttttatttc ttttttatct
     1681 ttttaaattt ttgattggct tgcttgagaa cttttttagt ttctttaatg tcacttgtaa
     1741 gcggtaaatc ctcaggttta attccactaa tttcaatcat ggactttctt actttgtgtc
     1801 caactgtttc agcgacattt tcaagatttc tttgcccttt aatattttca tttttgatct
     1861 tgagagtagt ttgagtaata cgaaatagat tagcggctaa ctcttctgaa cccatgaagt
     1921 caagtaggct tttattgaaa tcttccatgc ctttgtattc ttttaagtct tttaaatttt
     1981 tattatacaa cccacgatag ccggcatttt gaaaaagagg ataattcata actccttgcg
     2041 catgagccaa accactaata actttttctt tttggctaat ctcatctcta actaaaagtc
     2101 tttcaacact cttagataac tcttttgaat cttcaaaaag acttgcaatt ttagtaaaat
     2161 aaatctgtgc cttagcaaca ttcttttttt tgggatcggc attcatagaa atcaagaaac
     2221 aggcaaacct agaaagttta aaatcagtaa ctttcttccc atcaatttct cgctctactt
     2281 gtctaaaatc atcaatggta tcaatattca gagtatgaca agtcgtcatc gctttgctaa
     2341 taacaatacg aaaggcagaa aaatcttcat attctaagta agtcattaag tctctggcaa
     2401 accaataagt cataccattt tcttttccat agtcttcaaa ttgaatccta ccatcaaccg
     2461 cctgaaatac atccaattcc ttttccatat ctatagtttt attacaatga acgcaaggtc
     2521 aacgcttttt taaaggctaa ataattgcag gtcatttaat attaaaatgt gctcttgcta
     2581 tttcattcat gatgatagga taggcttctt tagcctcctc aacactacgt ccttttacat
     2641 ttgtccaata taagataaaa tcatcttctg tgcaatctat agaatatgat ttcttttcta
     2701 atactttctt ttcatcttca gtcatatact acctcatcaa tcctttagac ttaaaaattt
     2761 gatcgattag aatttcaatc tttttaaatt cctgatttga taaatataaa tcaacgccgc
     2821 tttgaatatg tataatattc gctttactca acaattcttt tttcaaaata ccaccttgct
     2881 tatttgcaaa gtattgctcc atgatacgtg taaagatttc tgaatcctgc gtatagtaag
     2941 agtataattc agagggtata ctattttgat ttgctagtaa atttttaaat gtagttgtat
     3001 ttttaatttc ggaaagtaaa ctttgcccct ctggagttct taaaaaagaa aaaccatttc
     3061 ctaaaacttt tgcttctaaa tgatgagccg tttcatgaac tactgtaagc attgcgtttg
     3121 cggaagattc atttaatgta ataactccat tgtcatatac acccgaagcc atactcttaa
     3181 gaggatttgt ttcactcata gcttggaact ggataggact ttgttcatat accttatcca
     3241 tatttat
//
LOCUS       DAOQOS010000260         3196 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_22236_length_3196_cov_7.014327, whole genome shotgun sequence.
ACCESSION   DAOQOS010000260 DAOQOS010000000
VERSION     DAOQOS010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3196)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3196)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3196
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22236_length_3196_cov_7.014327"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(88..270)
                     /locus_tag="PLF66_26270"
     CDS             complement(88..270)
                     /locus_tag="PLF66_26270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67620.1"
                     /translation="MRTLKPLPPESQIHMQEGQKLYKEKGMVRSVVILANTITKLQFK
                     RIAQETGIYQWERYID"
     gene            complement(559..1866)
                     /locus_tag="PLF66_26275"
     CDS             complement(559..1866)
                     /locus_tag="PLF66_26275"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HMZ67621.1"
                     /translation="MQFYHSLSAKFKILLFVSVALVLGFTVLSIVSISEILPLYNQLD
                     KNSYSIIDFLIKIGLSFVFVSIFCIFFVFKLLSRMLKPVEEISYLLERVANNDLQVDL
                     IDSTRKDEIGRMSRSIDSLVLGFSGVLTSLKYTGEQIGEAGKVFKKAASIFTDSLALQ
                     SKSSSDISESLNEMNQSLNLISVNFESSASSFRNIDSNLEDLAKSGSKILKGMDDLSN
                     FAKLSFDEGNIGEKNINAALVAMSQIKEKTNKITEFTSIISEISDQTNLLSLNASIEA
                     ARAGEYGRGFAVVAMEVTKLAEKTMNSVKEVKTLIHETNRTVNNGYAAVSDSAESLKR
                     LIENVHKIQSSSQRINVQIAKQEDNTVKISKNAKALSEFLDMIIENVELEKKVNDKMV
                     LSVREFAESFQSLEQESNGLRELSENLKEQSDGLLSIVNSFKL"
     gene            complement(1904..>3196)
                     /locus_tag="PLF66_26280"
     CDS             complement(1904..>3196)
                     /locus_tag="PLF66_26280"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HMZ67622.1"
                     /translation="NLGMYRIQFHDNNTTGMHIQIHRGGGFHYFEAEERNLSLPCHIY
                     VGGPPAMTLAAVAPMPEELSELIIASLLLGERLKITRDKKVSPLPIVSDSDFVFIGAI
                     PPKVRMPEGPFGDHYGYYALKHDYPIMKVHSLYHRKDAIWPATVVGRPPQEDHFIAEF
                     LQDLLSPMFPLVMPKVVSVWAYEESGVHSLAATVVKERYHKEAFTAALRILGEGHLSL
                     TKCLLVTNENISLKDFRNTFITILERIDLRTDVHILSNISQDTLDYTGPKVNSGSKMI
                     LLGIGDKKNELVPNFTGKLKNKSFHSPTVYCPGVLVVSGPKYKRGDKALEALLKETSI
                     RDFLFVFLVDDAKDTCRSDHDFIWNIFTRFEPAGDVTGKYDVIRNHIAYTNPIVIDCR
                     LKDWYPPVLVPDPSIVARVDERFGKILDQIQSPFEYSI"
BASE COUNT         1053 a          609 c          462 g         1072 t
ORIGIN      
        1 gattcctatt ttctaattca atacatctgg attttttcca ttctgaatcc aatctaaacc
       61 taacttttcc caaatcggat tcgattgcta gtcaatatat ctctcccatt gatagatacc
      121 tgtctcttgg gcaattcgtt taaattgcaa cttggtaatt gtgtttgcta atataacaac
      181 ggaacgcacc atgcctttct ctttatacaa cttctgtcct tcttgcatat gtatttgaga
      241 ttctggtgga agtggtttca aagtgcgcat atcaacgaaa actccaaagg aaccgttagc
      301 tgaatttaaa gcacttttac tttcttatac ccattttttc atttcttctt ctttgattgt
      361 atctacaaaa attaatttgt atccgtaaac ttccttacta attgaataca tagtaaaatt
      421 atcctcttgt tttgattttt ttatagtgga tacatatctc accaaaagtt atacttcaac
      481 aagattatac taattctggg tgttttggat catttataag aattagtata gactatagat
      541 aattataata aacttttttt aaagtttgaa actatttaca atcgatagta atccgtcaga
      601 ctgttctttt aagttttcgg agagttctct aagaccgtta gactcctgct ccaaagattg
      661 gaatgactca gcaaattcac ggacagataa aaccattttg tcgtttacct ttttttctaa
      721 ttccacattt tcaataatca tatctagaaa ttcggaaagc gcttttgcat tcttagaaat
      781 tttaacagta ttgtcttctt gctttgctat ttgcacatta attctttgac tagaagattg
      841 aatcttatga acattttcaa tcaaacgttt taagctttca gcagaatcgc tcacagcagc
      901 atagccattg ttgacagttc gatttgtctc atggattaat gtctttacct cttttacgct
      961 attcatagtc ttctcggcta gtttagttac ttccatagca actaccgcaa aacctctacc
     1021 gtattctcct gctctcgccg cttcaatact agcattcaat gacagcaaat tggtttgatc
     1081 agaaatttca gaaataatcg aagtaaattc agtaatttta tttgtttttt ctttgatctg
     1141 actcatagca actaacgctg cattaatatt tttctcgcca atattccctt catcaaaaga
     1201 caatttcgca aaattggaga gatcatccat accttttaga attttagatc cagattttgc
     1261 aagatcttct aaattagaat cgatatttct aaaagaagaa gcagaactct caaaatttac
     1321 actgattaga tttaaagact gattcatttc atttaaactt tccgaaatat cagaagatga
     1381 cttagattgc aaagcaagtg aatcagtaaa aatggatgca gcttttttaa aaactttacc
     1441 agcttcgcca atttgctctc ctgtatattt taaagaagtt aatacaccac taaatcccaa
     1501 gacgagtgaa tcaatagaac gactcattct cccaatttca tcttttcgag tagaatcaat
     1561 taaatctacc tgcaaatcat tattcgcaac tctttctaaa agatatgaaa tctcctccac
     1621 tggctttaac attcggctga gaagcttaaa aacaaaaaaa atacaaaata tgctcacaaa
     1681 aacaaaacta agtccaatct ttattaaaaa atcaatgatt gaataactat ttttatctaa
     1741 ttgattgtat aatggaagaa tttcagaaat cgaaacaatc gaaagaactg taaaacctaa
     1801 aactaatgca acagaaacaa agagtaaaat tttaaacttt gcgcttaaag aatgataaaa
     1861 ctgcatatgt aaattctact gttacagtta ttattgcaag caattaaata ctgtattcaa
     1921 atggactttg tatttgatct aggattttac caaaccgctc atccacacgg gctacaatgg
     1981 atggatcagg aaccaataca ggtggatacc aatcttttaa tctacaatca atcactatag
     2041 gatttgtata tgctatatga ttgcgaataa cgtcatattt tcctgtaaca tctcctgctg
     2101 gttcaaatcg agtaaatata ttccatataa aatcatgatc tgatctacag gtatcttttg
     2161 catcatctac taaaaacaca aatagaaagt ctcgaatcga tgtctctttt aaaagagctt
     2221 ctagtgcctt gtctcctctt ttgtatttag gtcctgatac aacaagaaca ccaggacaat
     2281 aaacagtcgg agaatgaaaa gatttatttt ttaatttacc tgtaaagttt ggaactaatt
     2341 catttttttt gtcaccaatt cctaggagta tcattttgct acccgaattt acttttggac
     2401 cagtatagtc caatgtgtct tgtgaaatat tagaaagtat atgaacgtca gttcttagat
     2461 caattctttc tagaatcgtt ataaaggtat ttctaaaatc tttcaaagaa atattttcat
     2521 ttgtaacaag taaacattta gttaatgata aatgaccttc tcctaatatt cgaagcgccg
     2581 ccgtgaaggc ttctttgtga taacgttctt tgacaacggt tgcggctaac gaatgaactc
     2641 ctgactcctc ataagcccat acagaaacta ctttaggcat aacaagcgga aacataggag
     2701 aaagtaaatc ctgcaaaaat tccgcaataa aatgatcttc ttgcggagga cgacccacaa
     2761 cagttgcagg ccaaatagca tcttttctat gatagagaga atgcactttc ataattggat
     2821 aatcatgttt cagagcataa tagccataat gatctccaaa aggtccttcc ggcattctta
     2881 ctttcggagg aatagctcca ataaaaacaa aatccgaatc agaaacaatc ggtagtggag
     2941 aaaccttttt atccctagta atcttcaatc tttcgccaag gagtaaagaa gcaatgatta
     3001 actcactcaa ttcttccggc ataggtgcta cagcagctaa cgtcatagcg ggaggtccgc
     3061 ctacatagat atgacagggt aggctcagat ttctttcttc cgcttcaaaa taatgaaacc
     3121 cccctcctct atgaatttga atatgcatac ctgtcgtatt gttatcatga aattgaattc
     3181 gatacattcc caaatt
//
LOCUS       DAOQOS010000261         3171 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_22540_length_3171_cov_4.790436, whole genome shotgun sequence.
ACCESSION   DAOQOS010000261 DAOQOS010000000
VERSION     DAOQOS010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3171)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3171)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3171
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22540_length_3171_cov_4.790436"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..421
                     /locus_tag="PLF66_26285"
                     /pseudo
     CDS             1..421
                     /locus_tag="PLF66_26285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004754730.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="carboxypeptidase"
     gene            718..1809
                     /locus_tag="PLF66_26290"
     CDS             718..1809
                     /locus_tag="PLF66_26290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011671054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HMZ67623.1"
                     /translation="MIFQKPRKLPHGKELLRTEKFTLIYLGSGNLHYSFIGEKGLVYG
                     DIDLKRNHFKIIPLITLATLITSYLVFGTNPTSAMIEPNPDVLQEEIAENDERDKRAK
                     HADENYLKQTEEQKLAVIKSSDSSKKSKNISYHVKDTDTLQSISSHFNVTPEAIREAS
                     NLKPTDKIYPGDVLSIPNRRGLLYKFKNGDTLAKVASTYRVSVEEIIEENKLEDSDIF
                     MPGQKIFLPGAIIPDPTPVWYSPVASNIITSGFGWRTFPRYQFHDALDLKSNYEPVRA
                     ARTGKVIYSGWMGGYGNVVILEHTGDLKTLYAHNSKLYVREGEFVLGGKIISRSGCTG
                     YCFGAHLHFEVIKNEKSVNPTNFIKGFTF"
     gene            1861..2949
                     /locus_tag="PLF66_26295"
     CDS             1861..2949
                     /locus_tag="PLF66_26295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020777387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HMZ67624.1"
                     /translation="MKILIYILIGLNLFYCATNQEIKDIRTQLNERIQNPYDTTTPVK
                     ILFSTNRKTISTDVSCSNNYYSNQVDTSEKLGSCEVVVPAQHDIGSLDQDPAGSTEKF
                     FKLENHESIGNIDDLVSRVSKNTFPEIIVFVHGFNVKFEEALLRAAQIKYDLKFSGEV
                     VLFSWPAGADDGVLSSLLIKNTYQNNLANAKASIFTFKSFLSKLASSNKKIHLIVHSM
                     GHQVVLPAVANLNREKNDKILKEMILNAPDFDYIEFKGIAENLKKSAERITVYCSPGD
                     NALVASSKVNANKRLGSCEKYPGLDMINVNPVDSPVMGIGGLGHGYYSSRPILTDVYQ
                     VILGLEAKNRLFLRKSSPINGEDYVMRR"
     gene            2957..>3171
                     /locus_tag="PLF66_26300"
     CDS             2957..>3171
                     /locus_tag="PLF66_26300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67625.1"
                     /translation="MKKIFLTILILLTFISNQPISTTDKEFVILYFCDMDGVYDFDLD
                     GRKGLATISEIKKSESKKFYDHRGQVF"
BASE COUNT         1114 a          511 c          615 g          931 t
ORIGIN      
        1 gggagatgtg atcaagcatg gaaccggtaa agcggtatta tccgcaggat tgcgaagacc
       61 agccgccgga aagactggaa ccacaaataa ctttagagat gcatggtttg taggatatac
      121 tcctgagctt gtaagttcag tttgggtggg atatgataca ggaacaattt ctcttggaaa
      181 agggatgtct gggggagtag tggcaagtcc tatgtggggg aaatttatgg cgggtgctct
      241 caagggcgag ccagtcaaag agtttaattt tggggaaaat ttaaatgtat ttacccgaaa
      301 ggtatgcact atctcgggta aaattccagg acctcattgt cataaggtat atgaggaagt
      361 gtttatcaaa gacactctag ataaagcaat ttgcgacgac catagaggct acaatccaga
      421 aatagatgtc ccacaggatt tacttcctgt tacaccaaca actgtgaata caaaagaaaa
      481 agaaccagta aagcctaaga aaaagaaacc taaggaaaca gattcgaaac cagaaaaacc
      541 agttgttgcc aaaaagaaac ctaagaagaa tattttccaa ggggatgagc gaatcgaata
      601 gcagaggtct ggaaaagtaa tatacttgtc tataaattcg tcgaaaatat aaaggactta
      661 tgttagcgaa aaggttaaat ttaaatttta ttcagattgg tttagaatga gtcggcaatg
      721 atatttcaaa aacctagaaa acttccccac gggaaagagt tactcagaac agagaaattc
      781 actttaattt atcttggatc aggcaatctc cattattcat ttataggaga gaaaggtctt
      841 gtctatggag atattgatct aaaaagaaat cattttaaaa ttataccgct gattactctc
      901 gctacactta tcacatctta tttagttttt ggaaccaatc ctacctctgc gatgatcgaa
      961 ccgaaccctg atgtattaca ggaagaaatt gcggaaaatg atgaaagaga taaaagggca
     1021 aaacatgcgg atgaaaatta tctcaaacaa acagaagagc aaaaactagc agtgatcaaa
     1081 tcctctgact cttctaagaa atctaaaaat atttcttacc atgtaaaaga cacagacaca
     1141 ttacaatcta tttcctctca ttttaatgta acccctgaag cgattcgaga agcatctaat
     1201 ttaaaaccaa cggataagat ttatccagga gatgttttaa gtattccaaa tcgaagagga
     1261 cttctctata aatttaaaaa cggagatact ctcgctaaag tagcaagcac gtatagagtg
     1321 agtgtcgaag aaattattga agaaaacaaa ttagaagact ctgatatttt tatgcctggt
     1381 caaaaaatct ttttaccagg agcaatcatt ccagatccaa ccccagtttg gtattcgcct
     1441 gtcgcttcta atatcatcac atctggtttt ggctggagaa catttcctcg ttatcaattt
     1501 catgatgcat tggatctaaa atcaaattac gagccagtgc gtgcggcgag aactggaaag
     1561 gtaatctaca gtggatggat gggtggttat ggaaacgtgg ttatattgga gcacacggga
     1621 gacttaaaaa cactctatgc tcataattcg aaattgtatg tgagagaagg tgaatttgtt
     1681 ttgggaggga aaattatttc tagatctggt tgcacaggct attgttttgg agcacatctg
     1741 cattttgaag tgattaaaaa tgagaaatcg gtaaatccta caaattttat aaaaggtttt
     1801 actttttaaa tcaaactcaa tcgtctctct aactttatta atttgaacag gaaggtatca
     1861 atgaaaatac taatctatat actcattggt ttaaatttgt tttattgtgc tacaaatcag
     1921 gaaattaaag acattcgaac acaattaaac gaaagaattc aaaatccata tgacacgact
     1981 acaccagtaa aaattttatt ttccacaaat aggaaaacaa tttcgactga cgtgtcttgc
     2041 tctaataatt actattcgaa tcaagttgat acttctgaaa aattaggttc ctgtgaagta
     2101 gtagtgcctg cacagcatga tataggaagc ctagatcagg acccagcagg aagcactgaa
     2161 aaattcttta aactagaaaa ccatgaatcc attggaaata tagatgattt agtctcacgt
     2221 gtttcgaaaa atacatttcc agagattatt gtttttgtgc atggatttaa tgttaaattt
     2281 gaagaagcac ttctaagagc agcacaaatc aagtatgatt taaaatttag cggagaagta
     2341 gttctatttt cttggcctgc cggagcagat gatggggttt taagttcact cttaattaaa
     2401 aacacctatc aaaataattt agcaaatgcg aaagcttcca tatttacatt taaatctttc
     2461 ttatcaaaac ttgcttcctc taataagaaa attcatctca tcgttcattc catgggacac
     2521 caagtagttt tgccagcagt cgcaaacttg aacagagaaa agaatgataa aattctaaaa
     2581 gaaatgattc taaatgctcc cgactttgac tatatagagt ttaaaggaat tgcagagaat
     2641 ctgaaaaaat cagcagagcg tataacggtt tattgttccc ctggagataa cgctcttgtt
     2701 gcttctagta aggtgaatgc aaataaaagg cttgggtctt gtgagaaata tcctggtttg
     2761 gatatgatca atgtaaaccc agttgattct cctgtgatgg gaattggtgg acttgggcat
     2821 ggatattatt cctctagacc gatcctaaca gatgtgtatc aagtcatact tggcttagag
     2881 gcaaagaatc gattattcct tagaaaatct tctccaatca atggagaaga ttatgtaatg
     2941 agacgataat caaactatga aaaagatatt tctaaccatt ttaattcttc taacattcat
     3001 atccaatcaa cctatctcaa ctactgataa agagtttgtt atattgtatt tttgtgatat
     3061 ggatggagtt tacgattttg atttagatgg tagaaaagga ttagctacaa tttcagaaat
     3121 taaaaaaagt gagtccaaaa agttttatga tcatagaggt caggtttttt t
//
LOCUS       DAOQOS010000262         3143 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_22876_length_3143_cov_5.612047, whole genome shotgun sequence.
ACCESSION   DAOQOS010000262 DAOQOS010000000
VERSION     DAOQOS010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3143)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3143)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3143
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22876_length_3143_cov_5.612047"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..582
                     /locus_tag="PLF66_26305"
     CDS             <1..582
                     /locus_tag="PLF66_26305"
                     /EC_number="4.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004783614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004419 - hydroxymethylglutaryl-CoA lyase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HMZ67626.1"
                     /translation="TKKNINRTIDESLDGFLDIVREAKKDKVKVRGYISTVIACPYEG
                     KISPEKVQSVVNRLQDMGVYEISLGETIGVGIPTEVEQLLELLLKKNKADFFAGHYHD
                     TYGMGIANVAKSIEMGLRSFDASSGGLGGCPYAKGASGNLATEDLLYFLNRAGFETGV
                     DLDLVVQASKFMETKLEKPLISKSYLAKIKILE"
     gene            583..1449
                     /locus_tag="PLF66_26310"
     CDS             583..1449
                     /locus_tag="PLF66_26310"
                     /inference="COORDINATES: protein motif:HMM:TIGR02757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02757 family protein"
                     /protein_id="HMZ67627.1"
                     /translation="MVNSINYRHYFHTLYSQYSSQTFLSSDPIEFCYRYEDPKDIEVI
                     AFISALFAYGNVVAIKKFLESLLSLLTNSPFSFIKRGDFGIVESKVSAYRFQTRKDIV
                     LFLNTLSMVLNQYNSLEPIFNIESKNENKLNIKIVNFQKYFLKQINLILGNKKISYGL
                     QFLIGSGKLNSAHKRYCMFLRWMVRDSFPDFGLYKSIRKEDLIYPTDVHIVRLASILG
                     ISNRKTVDFKLAESITQYFSDITKEDPLLYDFPLSRIGILKFCRAKYKKDVCEVCELK
                     KLCKVYKAAKLY"
     gene            complement(1467..2144)
                     /locus_tag="PLF66_26315"
     CDS             complement(1467..2144)
                     /locus_tag="PLF66_26315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002980167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67628.1"
                     /translation="MLEKGSKNNPTGNLIVYCYVIGENPFQPGCEIIASNVVVSFLKI
                     NDNFPVVTFPPISFPSIEDLKKVILPNESIYDMARLPDFRMPEDREAGNAYIQERMEQ
                     YNAFVMKYVELCKNKERNAMPDYNFEGVNEYLEALLTISMQFRSSTGLARDSVQQKMD
                     KLIHNFSERYPQFDLQNYKNALLFPGKKGDELASLYLKKFQAINDEKYETASIIKKRI
                     LDLETTV"
     gene            2333..2947
                     /locus_tag="PLF66_26320"
     CDS             2333..2947
                     /locus_tag="PLF66_26320"
                     /inference="COORDINATES: protein motif:HMM:TIGR01065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HMZ67629.1"
                     /translation="MTKEEKINVYTHGIGALMSFIGACILLYFALSTKDIWKIIGAFV
                     YSGSLVILFTISSIYHYKEGKQKFLFQKLDHIAIFYLIAGTYTPFILVNFREGMGPII
                     LSIVWVLAVIGTILKIFFTGKFDFLSTMVYLIAGWTVLLDIRNLISTLHPTGLFWLAA
                     GGLLYTGGVFFYLKESIPRNHEIWHGFVLGGAGCHYISVLFYVI"
BASE COUNT          988 a          467 c          553 g         1135 t
ORIGIN      
        1 acaaaaaaaa atataaatag aaccattgac gagtctttag atggtttctt ggatattgta
       61 agagaagcca agaaagataa ggttaaagtt cgaggttaca tctctactgt aattgcttgt
      121 ccatatgagg ggaaaatatc tcccgaaaaa gttcaatctg ttgtaaatag attgcaagat
      181 atgggtgtgt atgaaatttc tctcggagaa acgataggtg ttggaattcc aactgaagtg
      241 gaacaattgt tagagttact tctgaaaaag aataaagcag atttttttgc aggtcattat
      301 catgatacgt atggaatggg aatcgcaaat gtagcaaaat ccattgaaat ggggcttcgg
      361 tcttttgatg caagcagtgg aggacttggt ggttgcccct atgcaaaagg tgctagtgga
      421 aatttagcta ctgaggattt actttatttt ttaaataggg ctggatttga aacaggagtc
      481 gatttggatc tagtagtcca ggcttctaaa tttatggaaa ctaaactaga aaaacctctc
      541 atttcaaagt cctatcttgc taaaataaaa attttagaat aaatggtaaa ctctatcaac
      601 tatcgtcatt actttcatac actatactct caatattcaa gtcaaacctt cctttcatcg
      661 gatcctattg aattttgcta tagatatgag gacccgaaag acattgaagt aatagccttt
      721 atttctgctt tatttgctta tggaaacgta gtcgcaataa aaaagttttt agaatcattg
      781 ctttcattgc taacaaattc cccatttagt tttataaaaa gaggtgattt tgggattgta
      841 gagagcaaag tttccgcata tcgctttcaa actagaaagg atatagttct atttttaaat
      901 acactttcca tggttttaaa tcagtataat agccttgagc caatttttaa tatagaaagt
      961 aaaaatgaaa ataagctgaa tatcaaaata gtaaattttc aaaaatattt tttaaaacaa
     1021 atcaacttga ttttaggaaa taaaaaaata tcctatggcc ttcagtttct aattggtagt
     1081 ggtaagttaa attctgctca taaaagatat tgtatgtttc ttcgctggat ggtaagagat
     1141 tcttttccag attttggact ttataaatct atccggaagg aagatttgat ttacccaaca
     1201 gatgtgcata ttgttcgact tgcttccata ttaggaattt ctaatagaaa gacagtagat
     1261 tttaaattag cagaatcaat cactcaatat ttttccgata ttacaaaaga ggatcctctt
     1321 ttgtatgatt ttcctttgag tcgaattgga attttaaaat tttgtagagc taaatataaa
     1381 aaagatgtat gtgaggtttg cgagcttaaa aaattatgta aggtttataa agcggctaaa
     1441 ttgtattaac cgcaaattta aatcatttaa actgttgttt ccagatctaa aattcttttt
     1501 ttgataatag aagctgtttc atatttttca tcattaattg cctgaaattt ttttagataa
     1561 agactagcta attcatcacc tttttttcct ggaaagagaa gtgcattttt atagttctgt
     1621 aaatcaaatt gtgggtatct ttctgaaaaa ttatgaatga gtttatccat tttttgttga
     1681 acgctatcgc gagcaagtcc agtggaagat ctaaattgca ttgatattgt aagaagtgct
     1741 tctaaatact catttactcc ttcaaaatta taatctggca tagcatttcg ttctttattt
     1801 ttgcagagtt ccacatactt cattacaaat gcattgtatt gttccattct ctcttggata
     1861 taggcattac cagcctctct gtcttctggc attctaaagt cgggaagtct tgccatgtca
     1921 taaatacttt cattcggaag aattactttt tttaaatcct ctatagatgg aaaggagatt
     1981 gggggaaatg tgacaactgg aaagttatca tttattttta agaaactaac aacaacatta
     2041 gaggctataa tctcgcagcc cggttgaaaa ggattttccc cgattacata gcagtatacg
     2101 attaagtttc ctgtaggatt gttttttgag cctttttcca gcatgaatta cctcgcattg
     2161 tggagaatca ttccacttat aatttcagtt tttaaacttc cagtcaagga agaaaggttt
     2221 tttccctttt atatattaga tttttttatt tcaaaatata aacaaagaaa tttatatttc
     2281 tgaaactttg caacttttat cttgagtttg catcttatta ttcggagcgt atatgacaaa
     2341 agaagaaaag attaacgttt atacacatgg aattggtgct ctaatgagtt ttattggagc
     2401 ctgtatccta ctctattttg ccctttctac aaaagatatt tggaaaatta ttggtgcttt
     2461 tgtttacagt ggaagtttag tgattttatt taccatttct agtatatacc attataaaga
     2521 gggaaagcaa aaattccttt tccaaaaact agaccatatt gctatattct acctaatagc
     2581 aggaacttat acacctttta ttttagtaaa ttttagagaa ggcatgggac ccattattct
     2641 ttcaatcgtt tgggtcttag cagtcatcgg gacgattcta aaaatatttt ttacaggtaa
     2701 gtttgacttt ctgtctacaa tggtatattt aattgctggc tggactgttt tattagatat
     2761 tcggaatttg atttcaacac ttcatcctac gggattattc tggttagccg ctggaggtct
     2821 tctttatacg ggtggagttt tcttttactt aaaagaatca atccctagaa atcatgaaat
     2881 atggcacgga tttgttttag gaggtgcagg ttgtcactat attagtgtgt tgttttatgt
     2941 aatatagttc tgcccattaa tttttctttg cttgctttgg ataggcatgg tgtgatttct
     3001 ttacactatg cctgaattta ttccaccaat tccaaaggat ttccatggga gtatgggtga
     3061 aaaaattgtc tatgatgcat ttaggtccct cccagattcc tacttggttt ttcactcctt
     3121 cgcttggaca gaggaagaaa ata
//
LOCUS       DAOQOS010000263         3021 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_24555_length_3021_cov_6.511800, whole genome shotgun sequence.
ACCESSION   DAOQOS010000263 DAOQOS010000000
VERSION     DAOQOS010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 3021)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3021)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3021
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24555_length_3021_cov_6.511800"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(148..1704)
                     /locus_tag="PLF66_26325"
     CDS             complement(148..1704)
                     /locus_tag="PLF66_26325"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HMZ67630.1"
                     /translation="MNEKYLLEKKGEKASIYLRLFLLTVFSLGVIIGLIVKNEVPKII
                     GNYVIGILIYSFSIFLSLYNLRQEKFNVYLKYYGIAFELIGFAIVVSGYLRFNTVEEI
                     VAGIRNVTLFSVYLLLIAGASLRYSPLFSLISGLATTALFTTLSIVFYVFTNKAPGPG
                     VPINVTFIIINSLFVLAMAVTITTCTRYVRELVEEQIESKNLAHQQSENISRVIAEAQ
                     RAIQELNSVFTSTNQVVTTNKELNHEQSQLMDDISRIIDESNATTHSILSLTTNQESI
                     SDKNSNTLKELNSAMIAAEKVNQTISQKGSDALKRAEIGESELNNTVVEMENMKSISN
                     RVSHIVSIIYGIAKQTNLLALNAAIEAARAGEQGKGFAVVADEVSKLADLAGKNAKQI
                     GELVNEMNSATLKGANRIQTVVASIHDIVSVIRLIVSELFEMGEKVKQEMHLIRDVLE
                     HNSQMHEISNKLRITSTAQGEYSKEILHTIQDIYSRSKKILSTAEILEENTKVLNGIT
                     ERLNTSIHSN"
     gene            complement(1707..2612)
                     /locus_tag="PLF66_26330"
     CDS             complement(1707..2612)
                     /locus_tag="PLF66_26330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HMZ67631.1"
                     /translation="MLSFHHKRNKSIAILLGALVLIFIWSNHLFSISETLNEKNKLFI
                     ASLSIDSDVPETVRVSVVVKLKSHILERFGDSYNIISQSDLELLLKQVETFQKQGKDT
                     RDLITQINETRDADEMIHGKVFHDNGQIKLLLNNLKRDVKTKEFYTKSIVDISFFEND
                     FDFYIKEAAIKILNPRYKIKNPDSQVATNKKESNSKIDNSNFTETQITSLGKVGWGNF
                     EGLMKWEEAVDLCESKGMRLPTPEELRDMAQLKNHILREPCCVFWTSLSNKKDSDYAY
                     YVDINDGYGNFYHKDIDVRVRCVKK"
BASE COUNT          992 a          517 c          449 g         1063 t
ORIGIN      
        1 ttggctcacc caaactcaat cattgctttt gttaagcaat gataatgtaa ggtgatttaa
       61 taaatgcttt ttctaaagag atagaatgaa tatttgaatt ctatcctttc aaagcaatac
      121 ttgatttggc agttagatct aaaagaatta attagaatga atacttgtat ttaacctctc
      181 cgtgatccca tttaatactt ttgtattttc ctctaaaatt tcagctgtgc ttagaatttt
      241 cttggaacga gaatatatat cttggattgt atgtagtatt tctttgctat attcgccttg
      301 agctgtagaa gtgattctaa gcttattaga tatttcatgc atttgagaat tatgctctaa
      361 tacatctcta attaaatgca tttcctgttt tactttttcg cccatttcaa acagctcgga
      421 aacgatcaaa cgaattacac tgactatatc atgaatagac gcaactacag tttggattct
      481 gttcgcacct tttagggttg cagaattcat ttcattgaca agttctccaa tttgttttgc
      541 attcttcccg gctaaatcgg ctagtttact tacttcatct gcaactactg caaagccttt
      601 accctgttca ccagccctag cagcttcaat ggcagcgttt aatgctaata aatttgtctg
      661 ttttgcaata ccataaataa tcgatacgat atgcgatacc ctattcgaaa tagacttcat
      721 attctccatc tcgacgacag tattatttaa ttcagactca cctatttcag ccctctttaa
      781 agcatcactt cccttttgag atattgtttg attcactttt tcggcggcta tcatagcaga
      841 attcaattct tttaaagtat ttgaattctt atctgatata ctttcttgat tggttgtaag
      901 agataaaata ctatgagtag tagcattcga ttcatcaatg atacgactga tatcatccat
      961 taattgagat tgttcatgat ttaattcttt gtttgtagta acgacttgat ttgtgctagt
     1021 aaaaacagaa ttcagttctt gaatagctcg ctgtgcttct gcaataactc tagatatatt
     1081 ttcggattgc tggtgcgcta aattttttga ttcgatctgt tcttcgacaa gttccctaac
     1141 atatcttgtg caagtcgtga tagttaccgc catagccaga acaaataaag aattgataat
     1201 aataaaagta acattaatag gaaccccagg accgggggct ttatttgtga acacataaaa
     1261 aacaatcgac aaagtagtaa ataaagcagt agtcgcaaga ccgctaatca aagagaataa
     1321 aggagaatag cgaagacttg ccccagctat taaaagtaaa taaacactaa agagcgtaac
     1381 gtttcgaatg ccagcaacaa tttcctctac agtattaaat ctaagatagc cagaaactac
     1441 gatggcaaaa ccaatcaact caaaggcaat tccgtagtat tttaaataca cattgaattt
     1501 ttcctgccta agattataaa gggatagaaa aattgaaaaa ctgtaaatca gtatgccaat
     1561 gacataattt ccgataattt tgggaacttc gttttttaca atcaaaccaa taattacacc
     1621 aagactaaaa acagtaagaa gaaatagacg caaatatata gaggcttttt cgcctttttt
     1681 ctctaataaa tatttttcat tcatagttat tttttaacgc aacgaactct tacatcgata
     1741 tctttgtggt aaaaattacc gtatccatca ttgatatcta cataataagc ataatcagaa
     1801 tcctttttat ttgataaaga tgtccaaaac acacagcaag gctctcttag tatatggttt
     1861 tttaattgag ccatatctct cagctcttcc ggtgtgggta gtctcatacc tttactttca
     1921 caaagatcaa ctgcttcctc ccatttcatt agaccttcaa aatttcccca accaactttc
     1981 cctaatgaag tgatttgagt ttctgtaaaa ttagaattat caattttaga attggattct
     2041 tttttatttg ttgctacttg ggaatccgga tttttaattt tataacgtgg atttagaatt
     2101 ttaatagcag cctctttgat atagaagtca aaatcatttt caaagaaaga tatatcgact
     2161 atcgatttag tgtaaaattc tttcgttttc acatcacgtt taagattgtt tagtaacagt
     2221 ttaatctgac cattgtcatg aaaaactttt ccatgaatca tttcatctgc atctcttgtt
     2281 tcatttattt gagtgattaa atcacgggtg tcttttcctt gtttctgaaa agtttcaacc
     2341 tgtttcaaaa gtaattctaa atcagattgg gaaataatgt tatagctatc tccaaatctc
     2401 tcaagtatat gagattttag tttaacaact acacttaccc gaaccgtttc agggacatct
     2461 gaatcgatac taaggctagc aataaaaagt ttattctttt catttaatgt ctcagaaata
     2521 gaaaataaat gattggacca aatgaaaatg agaactagag ctccaagaag aatcgcgatc
     2581 gacttattgc gtttatggtg gaatgaaagc ataaatacaa attagtatgt aggattttaa
     2641 atttcaatct ataaatacta gatttctctg tctgttttta agacatcttt agatataaat
     2701 ccatacgtgt tccagaaaaa tgaatatttt tcataatttt aaaaagtatt tacttctgtt
     2761 ttaaatagtc ccattttgaa tgcagagaaa gcatcgttct aatgaagtaa aaaatatttg
     2821 cgagtcggtt tctacctaga tttcaaaact aattctcata acaaactatc attcatttca
     2881 ttctgagttt gagatggtag aattttactt actgttattc tactaaatag atagattcag
     2941 taactaatag agatgatttt aagaatatta gaaaattcgt aacctatcag gctgctaagt
     3001 tacttttatc aagagaatat a
//
LOCUS       DAOQOS010000264         2962 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_25447_length_2962_cov_5.471620, whole genome shotgun sequence.
ACCESSION   DAOQOS010000264 DAOQOS010000000
VERSION     DAOQOS010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2962)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2962)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2962
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25447_length_2962_cov_5.471620"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(126..1004)
                     /locus_tag="PLF66_26335"
     CDS             complement(126..1004)
                     /locus_tag="PLF66_26335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HMZ67632.1"
                     /translation="MQNKLILLLLSLTLIIFCKKETQNTETKIDTTTDRLVIADGGLH
                     LREKPDQASKSILLIPNHSIVTFLNETGEKKEINGYAAKWNQISYGDKTGYVYGAFIS
                     PVKILGTENNPKGDLTLTFYTILSDANPGDKNDCTQNYMAFGCVIVIRKSADNTLLSS
                     FDSKIAYHWIDNDHIAEEWIIGDAGYGGFGVRSVNVRTKAETDLWKREEMMDLGSPEE
                     SSEKNEFNRLCIKTICLDLNKNADILSQLKTNPNFKYYTKSTYKEKIDTYSTPISIDE
                     KKSIEYIPSKNIIIEN"
     gene            1423..2034
                     /locus_tag="PLF66_26340"
     CDS             1423..2034
                     /locus_tag="PLF66_26340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67633.1"
                     /translation="MKKIFFTLFLFCNLFLYADTPIFKPNYTLVGFGGIYVNSDLAPI
                     SFNLDFKYRPSYLWGMGLNRKINYRYSIFEFEAEGMLAKHTGAMNHYEVVGVGIARVP
                     KIFGSSFSFAVGEGQSVASENPKLENLRKGYDNGRIAFDDIQSRAWLNYLLFELDYTL
                     PWFNESSKVFARIHHRSGIYGVYCPPSPPCGSNFVVYGFKMDL"
     gene            2587..>2962
                     /locus_tag="PLF66_26345"
     CDS             2587..>2962
                     /locus_tag="PLF66_26345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67634.1"
                     /translation="MEKKSLIEVLPEIVKEGKREVEQIMERINSGTGLALQTNELVLP
                     AKDAAGSLYQGKLPEEPSWGTWLNRLIYGDNLLVMQALLTGDEQSNLPSMRGKIDLIY
                     IDPPFDSKADYRTKITLPGVDLE"
BASE COUNT          893 a          477 c          560 g         1032 t
ORIGIN      
        1 ctccccatca gccgagctat accgcaggct attgaattta ttcatttatt agcttcctct
       61 aatattctaa tttcctttat catacttatc aatatttatc ttttgcttaa ggttagttac
      121 ttatactaat tttcgataat tatattttta gatggtatat actcgattga tttcttttca
      181 tcaatggaga ttggagttga gtaggtatct attttttctt tgtaagtgga tttggtataa
      241 tatttaaaat ttggatttgt ttttaattga gagaggatat ccgcattttt atttagatct
      301 aaacaaatgg ttttgataca aagacgattg aactcatttt tctcagaact ctcttcaggg
      361 gaacctagat ccatcatttc ttctcttttc caaagatccg tttctgcttt ggtgcgcaca
      421 ttgacacttc gaactccaaa tcctccataa ccagcatctc cgataatcca ttcttcggca
      481 atatgatcat tgtcaatcca gtgataggca attttactat caaaggagga taacaaggtg
      541 ttatccgcag attttcgaat tactatgaca caaccaaatg ccatataatt ttgagtgcag
      601 tcatttttat cacctggatt ggcgtcggat agaattgtat agaaagttaa cgttagatct
      661 cctttgggat tattttctgt tcctaaaatt ttaacaggac taataaaggc tccatagaca
      721 tagccagttt tatctccata ggaaatttga ttccatttag ctgcgtaacc attgatttct
      781 tttttctcac ctgtttcatt taaaaaagtt acaatagaat gattgggaat taataaaatg
      841 cttttacttg cttggtctgg tttttctctc agatgtagtc ctccatctgc gatgaccaat
      901 cgatctgtag tagtatcgat tttagtttct gtgttttgtg tttctttttt acaaaaaatt
      961 attagagtaa gcgaaaggag gagtagaatc agtttatttt gcataggtca aatctatttc
     1021 tatgagtttt aattaaaaga aaaaaagtag agtgattcta aaagattacc accgattcac
     1081 accgatgaac acagataggc accgatggtt tggattatga ttatttaaaa attgtagagt
     1141 aggtcccatg acttgcgtta cttgtagttt tattttttta ccacgaagcg cacgaagagg
     1201 cacgaagaat ttttaaatgc tataaaactt agattgaata ccatttattt gttacgccaa
     1261 atagcgattt accacagata aagattcata aaatattgaa ttgaaaaatt tagataagca
     1321 ttttatcctt gtatcactct tagaaatcag gtatacctga ttttatatga gtgtatggat
     1381 ttttgggcag atttgaattt tatttaaatg gagatgagtt ctatgaagaa aatatttttc
     1441 acattgtttt tattctgtaa tctttttttg tatgcggata cacctatttt taagccaaat
     1501 tatacgttag tcggatttgg tggaatttat gtaaattcag atcttgctcc tatttctttc
     1561 aatttagatt ttaaatatag accttcttat ctttggggaa tgggattgaa tcgaaaaata
     1621 aattatagat attcaatttt tgaatttgaa gcagaaggga tgttagcaaa acacacgggt
     1681 gcgatgaatc attatgaagt ggtaggtgta ggaattgctc gtgttcctaa aatctttggt
     1741 tcctcgttta gctttgcagt aggggaaggt caatcggttg cgagtgaaaa tccaaaatta
     1801 gaaaatcttc gaaaaggata cgataatggt cgtattgcct ttgatgatat ccagtcccgt
     1861 gcttggctga attatctttt gtttgaattg gattatactc tcccttggtt caatgaatct
     1921 tctaaagtat ttgcccgcat ccatcatcgc tctggtatct atggcgtcta ttgtcctccc
     1981 tctcccccct gtggttctaa ctttgttgtc tatggtttta aaatggatct gtgagatgga
     2041 aagattacca ccgatagaca ccgatgaaaa agattaccac agatgaacac ggatggacac
     2101 cgataggcac cgatggtttg aggatcgggg ttatgatttt tcaaccatac ttcgacaccg
     2161 ctcagtataa actccgcaca cgaagaggca cgaagggttt taaaaaagat tacagcgggg
     2221 agtatgtttg aattgtgttc cgcgaataac gtttcagtat taacaaatat tgaattcatt
     2281 ctatttcttt aacgtttcaa agtcattgtc tgcaaatttt cgttagcagt tattttgaaa
     2341 attgattgct tccagtctag atcgtatcta gaggattttt gtttaaattg agtgaaaaga
     2401 ggaaaataac ttcgtataac atatgaagta tttggtgtta tatgaaataa tactattgac
     2461 atattaattg aaattcggag aagtatagtt atatgccatt ggcgcgctta actttttgtg
     2521 tgttcgcccc cttaagaagc ccggcgtccg tgccgggcta aagaaaagag gtaaaagata
     2581 aaatatatgg aaaagaaatc attaatcgaa gtcttacctg aaatagtaaa agaaggtaag
     2641 cgtgaagtag aacaaataat ggaacgtatt aatagcggga caggtcttgc tctacaaact
     2701 aatgaacttg ttttgcctgc gaaagatgca gcaggtagtc tctaccaagg taaattgccc
     2761 gaagaaccat catggggtac atggttgaat cgccttattt atggtgacaa tttactcgtt
     2821 atgcaggcat tactcactgg tgatgagcag tcaaacctgc cttcaatgcg agggaaaatt
     2881 gatctgattt atattgaccc accatttgac agtaaagctg attatcggac caaaataact
     2941 ttgcctggtg ttgatcttga ac
//
LOCUS       DAOQOS010000265         2918 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_26163_length_2918_cov_4.827803, whole genome shotgun sequence.
ACCESSION   DAOQOS010000265 DAOQOS010000000
VERSION     DAOQOS010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2918
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26163_length_2918_cov_4.827803"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1194
                     /locus_tag="PLF66_26350"
     CDS             <1..1194
                     /locus_tag="PLF66_26350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67635.1"
                     /translation="QMLIENAVKHNIATKDHPLAIFLDDWQWADLASLELLKVILLNP
                     EIKNTLIICAYRDNEVDKNHPFAITLEQIKSKNIVPKEIVVSELNLETVEEIVYQSLG
                     SQNKILAKMIHSKTKGNAFFLNEFLKSIHKENLLILDYRTGKWNPDLEKIQNLNITDN
                     VVDLIVSKVRKLNPDTLELLKYGTCIGSQFDIGLLSIICKQSNIDIIKKLETALIDGL
                     IFNTKSVTKYNFKIKHSSNSNSFSIYKFAHDKIQQAIYSILDEEERKKIHLQIGTLLL
                     ESSDSKEREERVFDITNQLNVAKELLIENSKKLLLAELILQSAKKVKSSTAYSSALLY
                     INQCKSLLDESAWINHYNIISDMYIEMAEIYYLNGDLEKSVDFINDSLNKVKTNIEKG
                     ELYNL"
     gene            1315..>2918
                     /locus_tag="PLF66_26355"
     CDS             1315..>2918
                     /locus_tag="PLF66_26355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67636.1"
                     /translation="MNKIERMKGDKQIKDFVDLPHMSSKETIVAMKILNYMVPPTFMT
                     LQPLFLVIAAKMVNLSLEYGNTGESSYGYAVYGIVLVEILQDYKNGFEYGAMALELSI
                     KLKNDIKKCLACEVLVGHLNHWHKPLSLSNELTEIGFKAGINSGELQFAGYIYLYQAY
                     NRFFEGANLQQYISEMEKFLEFTEKTKNQHASDVVNGCKLFAQYLSESKNNSYNLEIE
                     THFKNAKERQSNLFVEGPYYTFKAFISFLEGDYNSCILNSEIADEKKANVPGVIIIAE
                     NKYIRALGILSILKKNEKTDSEKEKYLNQIDCIQSELKILSDVSPENFYHKYTLIQAE
                     LAFYKKKFLDAMYLYKDSIQSAKENGFIQSEAIACELAAYFYLELNLEDFANVYFERS
                     LKCYKYWGANRKIDIMQNLFSKFQIYPYKGLEIPSKNESPFTIANYNSEIDINSIIKA
                     MQSISGEIVLEKLLTNMIQIIIENAGAEKGLLVLEAEAYIVHNSVCLYNSTPLEETKN
                     LPISMIQYVYRTGDNIVIYDAIKNIN"
BASE COUNT         1135 a          372 c          469 g          942 t
ORIGIN      
        1 caaatgctca tagaaaatgc tgtgaaacat aatatagcta caaaagatca tccacttgca
       61 atatttttag atgattggca gtgggcagat ttggcttctt tagaactatt aaaagttatt
      121 cttttgaatc ctgaaataaa gaatacttta atcatttgtg catacagaga taatgaagta
      181 gataagaatc atccatttgc tattacttta gaacagataa aatctaaaaa cattgtccca
      241 aaagagattg ttgtctccga gttaaaccta gaaactgtag aagaaattgt ttatcagtct
      301 ctaggaagtc aaaataagat attagcaaaa atgattcatt caaaaacaaa aggaaatgct
      361 ttcttcttaa atgaattttt gaaatccata cataaagaaa atttattaat attagattat
      421 agaactggaa aatggaatcc tgatttagaa aaaattcaaa atctgaacat aacggataac
      481 gtagtagatt taattgtatc taaagtaaga aagttaaatc cagatacttt agaattattg
      541 aaatatggaa catgtattgg tagccaattt gatatagggc tactgtcgat tatctgcaaa
      601 caatcgaata tagatataat taaaaaatta gaaaccgctc taatagatgg tttaatcttt
      661 aatacaaaat cggtaactaa atataatttt aaaattaagc attcctcgaa cagtaattca
      721 tttagtattt acaaatttgc acatgataaa atacaacaag ctatttactc aattttagat
      781 gaagaagaga gaaagaaaat acatctacaa attggaactt tattattaga atcgagtgac
      841 tcaaaagagc gagaagaaag agtctttgat ataacgaatc aattaaatgt agcaaaggaa
      901 ttattaattg aaaattctaa aaaactttta cttgcagaat taatcttaca atctgcaaag
      961 aaggtgaagt cttccactgc atatagttca gctctattgt atattaatca atgtaagtct
     1021 ttattagatg aatcagcttg gattaatcat tataatatta tatcagacat gtatatagaa
     1081 atggcagaga tttattattt gaacggtgat cttgaaaagt ctgtcgattt tataaatgat
     1141 tctctaaata aagttaaaac aaatattgaa aagggggaac tctataattt ataaataatt
     1201 gagtatactt tatccggtaa atataaagaa gcaatagact caggatacat cgctttaaaa
     1261 ctatttaata ttgatctacc atttgataat tataattcag cactgcaaac agaaatgaat
     1321 aaaatcgaaa gaatgaaagg tgataaacaa attaaagatt ttgttgattt gcctcatatg
     1381 tcttcgaaag agaccattgt tgctatgaag atcttaaatt atatggttcc acctactttt
     1441 atgacattgc aacctctgtt tttagttatc gcggcgaaaa tggtaaatct atcacttgaa
     1501 tatggaaata cgggtgagtc ctcttatgga tatgcggtat acggcatagt tttagtcgag
     1561 atcttacaag attataaaaa tggatttgaa tatggtgcta tggctctaga gctaagcata
     1621 aaattaaaaa atgatattaa aaaatgttta gcctgtgaag ttctcgttgg gcatctaaat
     1681 cattggcaca aacctcttag tctatccaat gaattaacag aaattggatt taaagcagga
     1741 atcaattcag gtgaattgca atttgctggc tatatttatt tataccaagc atataaccgt
     1801 ttttttgaag gagcgaattt acagcagtat attagtgaaa tggaaaagtt tttagagttt
     1861 accgagaaaa caaaaaatca acatgcatcg gatgtggtaa atgggtgtaa acttttcgct
     1921 caatatttat cagaatcaaa aaataattcg tataatttag aaatagagac acactttaag
     1981 aacgcaaaag aaagacagag taatttattt gtagaaggtc cttattatac atttaaagca
     2041 tttattagtt tcttggaagg tgattataat tcttgtattc ttaactccga aatagcagac
     2101 gaaaaaaaag caaatgtgcc tggagtgatt ataattgctg agaataagta tattcgagcg
     2161 ttaggtattc tctccatttt aaaaaagaat gaaaaaacag attcagaaaa agaaaaatat
     2221 ttaaatcaaa tcgattgtat tcaaagtgaa ttgaaaatac tctcagatgt ttctcctgaa
     2281 aatttctatc ataagtatac tctaattcaa gcagaactag cattctataa aaaaaaattt
     2341 ttagatgcca tgtatttgta taaagactct attcaatcag caaaagagaa tggctttatt
     2401 caaagtgagg caatcgcttg cgagttagca gcctattttt atttagaatt gaatttagaa
     2461 gactttgcta atgtctactt tgagcgatca ttaaaatgtt ataaatactg gggtgctaat
     2521 cgaaaaatag atattatgca aaatctattt agtaaatttc aaatctatcc ttataaaggg
     2581 ttagagattc cttccaaaaa tgaaagtcca tttactattg ctaattataa ttcggaaata
     2641 gacataaact caattataaa agcaatgcaa agtatttctg gagaaattgt tttagagaag
     2701 ttacttacta atatgattca aatcattatt gaaaatgcag gagctgaaaa aggactctta
     2761 gttttggagg ctgaagcata tatcgttcac aattcagttt gcctatacaa ttcaacacct
     2821 ttggaggaaa caaagaatct acccatttct atgattcaat atgtttatag aacgggggat
     2881 aatattgtta tttatgatgc gataaaaaat attaattg
//
LOCUS       DAOQOS010000266         2793 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_28333_length_2793_cov_4.933528, whole genome shotgun sequence.
ACCESSION   DAOQOS010000266 DAOQOS010000000
VERSION     DAOQOS010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2793)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2793)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2793
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28333_length_2793_cov_4.933528"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..861
                     /locus_tag="PLF66_26360"
     CDS             <1..861
                     /locus_tag="PLF66_26360"
                     /inference="COORDINATES: protein motif:HMM:NF014680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HMZ67637.1"
                     /translation="RMEQAKETVSLKEQISILEKLNSALTEEAKNLSNALRGDSKARG
                     NWGEGILERILDMSGLEKGRDFNVQQSFSSEENQRYTPDVIVNLPDNKHLVIDSKVSL
                     VAYDNYCAADSESKKSQYLKEHINAIYARIKELSSKDYFKLKGLNTPDFVLMFIPIEP
                     SFSIAMQEDPGIYNHAFDNNILIVTATTLIGALRVINVIWKQEKQNRNVVEIAKEAGL
                     MLDKFQGFLDDMKKVGDHISNTEKTYQSAMSKLKTGKGNLIGRAQKIQSLGGKGSKTL
                     SEDFLGYDEE"
     gene            complement(870..1307)
                     /locus_tag="PLF66_26365"
     CDS             complement(870..1307)
                     /locus_tag="PLF66_26365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67638.1"
                     /translation="MDYSNINEYPVAIEFFEEGGPVASKYKYLTHIRVFSKTNKIYLS
                     YKEAREFISPKPRLVVSNEKELPLDVYKDFLNDLISHKVDELNKIFPNAKANGKANFF
                     SFQLGNVFQIRFEYSNLDKETAEFKPYENILQIMRNLLFSNMR"
     gene            1606..2028
                     /locus_tag="PLF66_26370"
     CDS             1606..2028
                     /locus_tag="PLF66_26370"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HMZ67639.1"
                     /translation="MNSKPESHSSSDTIAILVVDDNLINRNVMQKIFLKLGYSTDTAE
                     HGLAALEIISKKRYDLIFMDIQMPVMDGLDATREIRRKLGHASPIVIALTANIRNQSN
                     EEGMDDYLTKPITLEDIRVVIEKWNDQIYEKREKFSKQ"
     gene            1985..>2793
                     /locus_tag="PLF66_26375"
     CDS             1985..>2793
                     /locus_tag="PLF66_26375"
                     /inference="COORDINATES: protein motif:HMM:NF013106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic peptidoglycan transglycosylase"
                     /protein_id="HMZ67640.1"
                     /translation="MIRFMRRERNFRNNKTFLLILFFASFLSLANSIYPQEIPDFQTV
                     KKSILVSDMIIYDKNRNILHEIRNNKWNRRLDWVSLDKISIAVIHSLLIAEDRRFFDH
                     KGVDWKALADGAFRFFTFRSRRGGSTLTMQLASFLNVSLKGNSGGRTFTQKLDQILYA
                     QKIEQSWTKEEILEAYLNLANFKGELVGIEAASRALFQKQPHGLDEMEASIIVSFFKS
                     PSGKESRIADRACYIASELEKKIDCDKIKEKVKELYRKNYFIKPKYDLIYH"
BASE COUNT          990 a          422 c          479 g          902 t
ORIGIN      
        1 agaatggaac aggcaaagga aactgtctct ttaaaagaac aaatctcaat tcttgaaaaa
       61 ttgaattcag ctttaaccga agaagctaaa aacttatcca atgcgcttcg tggtgatagt
      121 aaagctcgtg ggaattgggg agaaggtata ctggaaagaa ttctagatat gtctggttta
      181 gagaaaggaa gagattttaa tgtgcaacaa tctttctctt cggaagaaaa tcaacgttat
      241 acgcctgatg ttatcgtaaa cctaccagat aataaacatc tagtaattga ttcaaaagtc
      301 tctttagtag cctacgataa ttattgcgca gctgattctg aaagcaaaaa aagtcaatat
      361 ttgaaagagc atataaatgc aatttatgcc agaattaaag aactctcttc aaaagactat
      421 tttaaattaa agggattaaa tactcctgat tttgttttaa tgtttatccc aattgaacca
      481 tctttctcaa ttgctatgca agaagatcca ggtatttata atcatgcttt tgataataat
      541 atattaattg taacagccac gactttaatt ggggcattac gggtaatcaa tgttatctgg
      601 aaacaagaaa agcaaaatcg aaatgttgta gaaattgcga aagaagccgg actcatgtta
      661 gataagttcc aaggattttt agatgacatg aaaaaagtag gcgatcatat ttcgaataca
      721 gaaaaaacct atcaatcagc tatgagcaaa ttaaaaacag gaaaaggaaa tttgataggt
      781 cgtgctcaaa aaattcaaag tcttggtggt aagggatcaa aaactctctc tgaagatttt
      841 cttggatatg atgaggagta aacggctatt taccgcatat ttgaaaaaag caaatttctc
      901 ataatttgta gaatgttttc atagggttta aattctgctg tttccttatc taagttggaa
      961 tattcaaatc tgatttgaaa aacatttcct agttggaagg aaaagaaatt tgcttttcca
     1021 tttgctttcg catttgggaa tattttattt agctcatcta ctttatggct gattaagtca
     1081 ttcaggaaat ctttgtaaac atctaacgga agttcttttt cattggatac tacaagcctt
     1141 ggtttaggac taataaattc tctcgcctct ttatagctca gataaatctt atttgtctta
     1201 ctaaagactc taatatgggt aagatattta tactttgaag caacaggtcc accttcttca
     1261 aagaactcta ttgcgaccgg gtattcattt atattcgaat aatccaattt tagttacttt
     1321 tcctttgtaa aatatacact tatacagagt gttaagattt tccagaactt tttttgatac
     1381 tcatattctt cttatcgact cttttgtttt gggattgaaa gctagtttac tctttatccg
     1441 catttatctc tataattcga atgcatttca acgaatgcaa aggctataaa tccaaaacct
     1501 cgaaatctaa aagaaaataa atcggaaaat attctctaga aataatatag cttgcaatag
     1561 gattgatctt tttatactat tcttttgctt gttcgccaaa atcctatgaa ttccaaacct
     1621 gaatcccact catccagcga tactattgca attctagttg tagatgataa tctaataaat
     1681 agaaatgtaa tgcaaaaaat cttcctgaag ttgggatatt caacggatac agcagagcat
     1741 ggacttgctg cattagagat tatttcaaaa aaaaggtatg atctaatttt tatggatatc
     1801 caaatgcctg ttatggatgg cttagacgca acaagggaaa ttcgtagaaa attaggacat
     1861 gcgagcccta ttgtaattgc ccttactgca aatataagaa accaatcgaa tgaagaagga
     1921 atggatgact atttgacaaa accaatcact cttgaggata ttcgagtagt tattgaaaaa
     1981 tggaatgatc agatttatga gaagagagag aaattttcga aacaataaaa cttttttact
     2041 tatccttttt tttgcttctt ttttgtcttt ggctaattcc atttatcctc aagaaatacc
     2101 tgactttcag actgttaaga aatcgatact tgtttcagac atgattatct atgacaagaa
     2161 tcgaaatatt ctacatgaaa ttagaaataa taaatggaat cgaagattag attgggtatc
     2221 tctagataaa atttctattg cagtcataca ttcactccta attgctgagg atagaagatt
     2281 ttttgaccat aaaggagttg attggaaagc gttagcggat ggggcatttc gattctttac
     2341 atttcgatct agacgaggag ggagcactct cacaatgcag ttagcttctt ttttaaacgt
     2401 tagtttaaaa ggtaattcag gaggcagaac ctttactcaa aaattagacc aaattttata
     2461 tgcacaaaag atagaacaat cctggactaa ggaagaaatc ctagaagcat atttaaattt
     2521 agcaaatttt aaaggagaat tagttggtat tgaagcagcg tctagagcct tgtttcaaaa
     2581 gcaacctcac ggactagatg aaatggaagc tagtataatt gtctcctttt ttaaatctcc
     2641 ttctggtaag gaaagtcgaa ttgcagacag agcctgctat attgcaagtg aactagaaaa
     2701 aaaaatagat tgtgataaaa tcaaagaaaa agtaaaagaa ctatatagaa aaaattattt
     2761 tattaaacct aaatatgatc ttatctatca ttt
//
LOCUS       DAOQOS010000267         2741 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_29299_length_2741_cov_6.697692, whole genome shotgun sequence.
ACCESSION   DAOQOS010000267 DAOQOS010000000
VERSION     DAOQOS010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2741)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2741)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2741
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29299_length_2741_cov_6.697692"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(148..1269)
                     /locus_tag="PLF66_26380"
     CDS             complement(148..1269)
                     /locus_tag="PLF66_26380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67641.1"
                     /translation="MKKIIFLILISFLSLEAFKLSGKKWSGLHPVIQIEIDPFFQGIK
                     THQGVKFFLKDILLAVNEWNRKTGSHVNWKIGSLAVPDTKSYFDLVNADKIANDRGQS
                     LCEVVKSGTGKIASVVSSEKDSDGDCTANSCVYVWSCGDEIVHADVTLNNSAYSWADL
                     ISDQNTFNIRSEILKILGQLSGLTSCSPGDRDSDCSKSGDPDMQSVLYKFPDFGKKEV
                     ITSDDISGIQTLYGTFNLPFPQTGPYALNDQERGIVQDLIDLENKLGYANPEGRKAIA
                     VQLNHLAKYSEYRSKKDLKQQYDELMNIMQSQTPTLSIEGLKIQRDLLLMGIVTATKT
                     KEDVMNGFSSMDIGFIDYTIQRHMELWRMTIDRVGEREQ"
     gene            complement(1280..2593)
                     /locus_tag="PLF66_26385"
     CDS             complement(1280..2593)
                     /locus_tag="PLF66_26385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67642.1"
                     /translation="MNFVVGYDFIESLFYNPSFTGVEIPPANQVLLEIDHVDILTSGT
                     PITIYPTVKESKVIIYREKHYFPFFTGLILPVGDYSGVNVYLKNNGKVIIQDREYSLV
                     IDTPIVSYSDTFSVQLGKVTTLESATLKDYNDGLAGNRRTPTRGTFEKVLSPEFVYQT
                     NAAAIQHMYINIVNRAANIHDSLTKLNINVESLSASSSGNSSFVLNRTPTQFEVLQLR
                     NGYVGLAGANQIEAGNYEYFELALARTHSAEATGGIPIQLYMDEASQNIFRFYGPFVL
                     RGGETFETYLHLDPNRSVYFVKDHGYVIDPTVSLLATASMNPSEEEIKIINTVGKFMN
                     ILASESDIIIRGTVTSVTPLVAPNASGRNLIYSDVVLNVTKSLKGNVDIGPFSLRVPG
                     GTYNGIMLEVSNMPKFKASEEVLIYLYKQGSTWKVTRGNLGKINL"
BASE COUNT          856 a          535 c          412 g          938 t
ORIGIN      
        1 atccccaaga caaaccaatc aattcgagta atgcagtttt attatcatca ttacgctttt
       61 ttctttctga cgcgtttgta tcaaaacatt taactaaaaa tgatgtcgaa aaaattaata
      121 gtaacatata ttttatctta cgatttatca ttgttccctt tctcctactc tatcaatcgt
      181 catacgccaa agctccatat gtctctgaat tgtataatca atgaatccaa tatccatcga
      241 actaaaccca ttcatcacat cttctttagt ttttgtagct gtaacaattc ccattaataa
      301 taaatctctt tgaattttca aaccttctat tgataaagtt ggggtttgac tttgcattat
      361 gttcattagc tcatcatatt gttgttttaa atctttcttt gacctatact cactgtactt
      421 tgccaaatga tttaattgta ctgcaatagc ctttcttcct tcaggatttg cataacctag
      481 tttgttttca aggtctatca aatcttgaac aattcctctc tcttggtcgt ttagcgcata
      541 aggacctgtt tgcggaaatg gtaaattgaa ggtaccatac aaagtttgta ttccactaat
      601 atcatctgat gtaatgactt ctttttttcc gaaatcagga aatttataaa gtacgctctg
      661 catatcggga tcgccagatt tactacaatc agaatctcta tcaccagggc tacagcttgt
      721 tagccccgat aattgaccta gaatttttag aatttctgat ctaatattaa atgtattttg
      781 gtcagaaatt aagtctgccc aactgtatgc ggaattattt agagtgacat ctgcatgaac
      841 aatttcatca ccacaagacc aaacatagac acaagaattc gcagtacaat ccccatcaga
      901 gtctttttct gacgaaacga cagatgctat ttttcctgtt ccagatttta caacttcgca
      961 aagagattgt cctctatcat tagcaatttt gtcagcattg actaaatcaa aataagattt
     1021 tgtatcaggc acagctaaag agcctatttt ccagttcaca tgtgatcctg ttttacggtt
     1081 ccattcatta actgccaata gtatatcttt taaaaaaaat tttactccct gatgtgtctt
     1141 gataccttgg aagaaagggt ctatctctat ttggataaca ggatgtaaac cactccattt
     1201 ttttccagaa agcttaaagg cttctagcga aagaaagcta atcaaaatta aaaaaataat
     1261 ttttttcatt cgattgcctc tataaattta tttttccaag atttcctctt gttactttcc
     1321 aagtagaacc ttgtttgtat aaataaatta aaacttcttc actagcttta aacttaggca
     1381 tattcgatac ctcaagcatt attccgttat aagtaccacc aggaactctt aaactaaagg
     1441 gaccaatatc tacatttcct ttcagagatt tggttacatt caacacaaca tctgaatata
     1501 ttaaattcct tccactggca tttggtgcta ctagcggtgt cacagaagta actgtccctc
     1561 taatgataat gtctgactct gatgcaagta tattcataaa tttgccgaca gtattaataa
     1621 tttttatttc ctcttcactt ggattcatag atgctgtagc aagtaaacta acggttggat
     1681 caataacata tccatgatct tttacaaaat atacagaccg attcggatct aaatgtaaat
     1741 aggtttcgaa tgtttcccca cctcttagaa caaaaggacc atagaatcta aatatattct
     1801 gactcgcttc atccatatat aactggatag gaataccgcc tgttgcttct gcactatgag
     1861 ttctagctaa agctaattcg aaatactcat aattacctgc ttctatttga ttagcccctg
     1921 caagaccgac ataaccattt cgaagttgaa gcacttcgaa ttgagttggt gttcgattta
     1981 aaacaaaact agaatttcca ctgcttgatg cagaaagaga ttctacattg atatttaatt
     2041 tagttagtga atcatggatg tttgctgcac gattcacaat gtttatatac atatgctgaa
     2101 ttgccgcagc attagtttga taaacgaatt caggggataa aactttttca aaagttcccc
     2161 ttgtcggagt tcgcctattt ccggcaagcc cgtcattata atcttttaat gtcgcagatt
     2221 ctaatgtagt tacttttcca agttgaaccg agaaagtatc ggaataagat acaataggag
     2281 tatctataac taatgaatat tctctatcct ggattattac ttttccatta ttctttaaat
     2341 atacgttaac tcctgaataa tccccaactg gtaaaattag tcctgtaaaa aaaggaaagt
     2401 agtgtttttc tctataaata ataactttgg attcttttac tgttggatag attgtaatcg
     2461 gagtccccga agtcaatata tcgacatggt ctatttcaag taatacttga ttagcaggtg
     2521 gaatttccac tcctgtaaat gaaggattgt aaaataaaga ttctatgaag tcatacccaa
     2581 caacgaagtt cacataacgt ttaagcgtat ctgacgtgac tttgaaactc gaaagtgcga
     2641 tagcaccttt tgagtttcaa agtcatgtct ccgagccacc cgtataacta gcaagtcctg
     2701 tgactgaacg gatggcacgc aaacttgctc ttttctgcaa g
//
LOCUS       DAOQOS010000268         2729 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_29537_length_2729_cov_4.863126, whole genome shotgun sequence.
ACCESSION   DAOQOS010000268 DAOQOS010000000
VERSION     DAOQOS010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2729)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2729)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2729
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29537_length_2729_cov_4.863126"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(284..592)
                     /locus_tag="PLF66_26390"
     CDS             complement(284..592)
                     /locus_tag="PLF66_26390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67643.1"
                     /translation="MKKPLIIFLLIIIGFICDPVEENDITMLDLRKNSTLLLKKLKGK
                     EKILLSFRGKPIATIHPISEESDWETDPFLQFLNSIPESPKKKTRISNKEIDSIIYGI
                     "
     gene            complement(609..1376)
                     /locus_tag="PLF66_26395"
     CDS             complement(609..1376)
                     /locus_tag="PLF66_26395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67644.1"
                     /translation="MKKLKNILISILVFAGFINISPETFFYDKQACFSIGNQNWKVEQ
                     VGGRILMKQRIDSGTNTARLIFYFRNENDLKLFRKPWQEGKYTGKEFTFLDKNIFNYN
                     ESWELTQSNLSFPFQLPSKYLSFELLKDGGWYGNFVDYQFCYEDYCVNINEHVLYTNN
                     MMLDAFNYDNKKKIIYIRTIPSEWRDAGGYKTYFHRVYESIMIGKNKPVRFLLVYIHS
                     YNFKNGHQRMRLENVYHPEARQWFETFRELDTCSGAN"
     gene            complement(1731..>2729)
                     /locus_tag="PLF66_26400"
     CDS             complement(1731..>2729)
                     /locus_tag="PLF66_26400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012389470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HMZ67645.1"
                     /translation="ATKGSGTIIGELALLDKSPRMASVRAVEPCRVVGIGAKEFMDLV
                     TTQPAIANGVLQDVVARWRQNEGILKKREKELQQAKQLIELQRQKSEELLLNILPKAV
                     ADELKLNGATQPLHYDSVTVIFTDFKGFTKISEQMTPEELVSRLDVFFTSFDELADKF
                     KLEKLKTIGDAYMCAGGLPTVNKTHPVDACLFALQVQYIMRVAKELKQSMDLPFWELR
                     LGIHTGPVVAGVIGKKKFAYDVWGDAVNTSARMESSGEPGRINISGRTYELVKDFFEF
                     EYRGKIEAKNKGKIDMYFLNSLKKDLSDAEEGHVPNQKFWELYNKTFAIDTIAEGI"
BASE COUNT          834 a          510 c          399 g          986 t
ORIGIN      
        1 tttaaaattg cggcaaattt ggattagcca gtatagaaaa agaaaatcca tatatgtctt
       61 gatatttata cctatcaatc gaacagcttt atgaacccag cttttgtgaa atgtttatct
      121 gttgtaacag cctttgtaat tctaaattct ttcatcaaaa taaaggatga tacatctatg
      181 aaagaataac cctgttcgat atgttttgaa aaatattctc ctgatttttg aaatcttgct
      241 tcatctattc tttctatccc agaatggata ctttgcgtct ttgttagatg ccataaatta
      301 ttgaatcaat ttctttgttg gaaattctag tcttcttttt aggactttcc ggaatggaat
      361 taagaaattg taaaaaagga tcagtctccc aatccgattc ctcagaaata ggatgaatgg
      421 ttgcaattgg ttttcctcga aaggaaagaa gtattttttc ttttcccttc agcttcttta
      481 atagaagtgt agaatttttt cgtaaatcca acatagtaat atcgttctct tcaacagggt
      541 cacaaatgaa tccgataata atcagcagaa aaatgatcaa aggttttttc atagaaaatt
      601 ttcattattt agttggctcc actacatgta tccaattcac gaaatgtttc aaaccattga
      661 cgagcctcgg gatgataaac attttccaat cgcattcttt gatgcccatt tttgaaatta
      721 taagaatgaa tataaaccaa aagaaatcga actggtttat tcttaccaat cataatactt
      781 tcgtatactc gatgaaagta tgttttatat ccacctgcat ccctccattc cgaaggaata
      841 gttcttatgt atatgatctt ttttttgtta tcataattaa atgcatccag catcatatta
      901 tttgtataaa gtacatgttc gtttatattc acacaatagt cttcatagca aaattgataa
      961 tctacaaaat tcccatacca acctccatcc ttcagtaatt caaaactcaa atacttggaa
     1021 ggtaattgaa atggaaaaga taaattactt tgcgttaatt cccaagactc attataatta
     1081 aaaatattct tatccaaaaa cgtaaactct ttaccagtat attttccttc ttgccaaggc
     1141 ttcctaaata attttaaatc attttcattt ctaaaataaa aaattagcct agccgtattc
     1201 gttccagaat caattcgttg tttcattaaa atacgtcctc ccacttgttc taccttccag
     1261 ttttgatttc ctattgaaaa acaagcctgt ttatcataga aaaaagtttc tgggctaata
     1321 ttaataaatc ccgcaaatac taaaatagat atcagaatat tttttaattt tttcatataa
     1381 tttaccaatc aattttattc tctaaccgtt ttttctaaaa ttataaatta cttattcgtt
     1441 atgttagatt tttttatcct gtcttatttc agaaagattt ccagaccgaa ttccagttca
     1501 ttgaaagtcg aatatcttag aaaattcatt cggtatgaat tacttcatac aaatagtagg
     1561 gttgaaaatt tttcgacgtc aagagataaa aaataaataa cgcattgttt ctcccatttc
     1621 atatttcgtc gtgatccttc gtgttcttcg tggttaaatt ttatttaaaa gatttgaact
     1681 taatgaaaca aactatctgt gttcatccgt gtctatccgt ggtgaatctt ctaaatccct
     1741 tcagcgatag tgtctatagc aaaggtcttg ttgtagagtt cccagaattt ttgattggga
     1801 acatgacctt cttctgcatc tgataaatct tttttaagag agtttaaaaa atacatatcg
     1861 atcttgcctt tgtttttggc ttcgatcttt cctctgtatt caaattcaaa aaaatctttt
     1921 acgagttcgt aggttcttcc tgaaatattg atccgtccag gttctccact tgattccatt
     1981 cgagcagaag tattgacagc atcaccccaa acgtcatacg caaacttctt ttttccgatt
     2041 acccctgcta caacaggacc cgtatggatt ccaagacgga gttcccagaa tggtaaatcc
     2101 atcgattgtt tgagttcttt tgctactcgc ataatgtatt gcacttgtaa agcaaataga
     2161 caggcatcta caggatgcgt tttattcact gtgggaagac cacctgcaca catataagca
     2221 tcgcctatcg tttttaattt ttctaatttg aatttatccg ctagttcatc aaaggaagta
     2281 aaaaatacat cgagtctact cacaagttct tcaggagtca tctgctcact gattttagta
     2341 aatcctttga aatcagtaaa gatcacagta acagaatcat aatgaagtgg ctgtgtcgct
     2401 ccatttagtt ttaactcatc tgcaacggct ttcggtaaaa tatttaaaag tagttcttcc
     2461 gacttttggc gttgtagttc gattagctgt tttgcttgct gtaattcttt ttctcttttt
     2521 ttgagaatgc cctcattttg acgccaacgt gctactacat cttgtaatac cccatttgcg
     2581 atggcaggtt gtgtggtgac taaatccata aattcttttg ctccaatgcc tacaactctg
     2641 catggttcaa cggctcttac acttgccata cgtggggatt tgtctaatag tgccaattct
     2701 ccgattatag tgccagaacc ttttgtagc
//
LOCUS       DAOQOS010000269         2597 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_32202_length_2597_cov_5.929190, whole genome shotgun sequence.
ACCESSION   DAOQOS010000269 DAOQOS010000000
VERSION     DAOQOS010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2597
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32202_length_2597_cov_5.929190"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..439
                     /locus_tag="PLF66_26405"
     CDS             <1..439
                     /locus_tag="PLF66_26405"
                     /inference="COORDINATES: protein motif:HMM:NF019235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1564 family protein"
                     /protein_id="HMZ67646.1"
                     /translation="SVSTLLVPAHLVPFLKSKSLSFGSLNTYFKFLLSQYRIITHSGL
                     IPYPASIKTQFQKNNLGLTRVSFRVDNDDWIELGELSIAFGKSRCWLFTYLLELDFLG
                     LWEILSKTDLINEVPTVDRLGLRASWSFRRTDYIYKRSYYVRI"
     gene            571..1149
                     /locus_tag="PLF66_26410"
     CDS             571..1149
                     /locus_tag="PLF66_26410"
                     /inference="COORDINATES: protein motif:HMM:TIGR04455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MXAN_6521/LA_1396 family lipoprotein"
                     /protein_id="HMZ67647.1"
                     /translation="MQNIFLFLLFLVIQCSSVQYVKKNPTLKDDISSTKRIAIVSAKD
                     SNISKNEYFMLHDMLRQEISHHTLFIVYKAPTKFKNICSKEFPKVEGVLIAEMKEYSE
                     ASKSKFELKAALRKCSTDSTIWSAKVADKFSLEANKELSLVKGYVGKYGDSVANKVNA
                     FFQLSKDLVSELQGPEKLSEEEELEKIEVESE"
     gene            1208..>2597
                     /gene="fumC"
                     /locus_tag="PLF66_26415"
     CDS             1208..>2597
                     /gene="fumC"
                     /locus_tag="PLF66_26415"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004778917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HMZ67648.1"
                     /translation="MATRKETDSMGEILVDDSKYWGAQTERSLHHFKIGTDKFPREMI
                     RALGVLKKSAAIVNAELGILPADKRDLIVKVADEVISGVLDEHFPLSIWQTGSGTQTN
                     MNANEVISNRAIELAGGIKGSKKPIHPNDDVNKAQSSNDTFPTAMHIAAAEQLNKRLI
                     PTLTKLRDTLKKKSEDFKDIIKIGRTHLQDATPLTLGQEFSGYVQQLTYGIERVKHVL
                     PSVYRLAIGGTAVGTGLNTHPEFAEKVAKQIAKETGLPFVSAENKFEALAAHDSLVEA
                     SGVLKTLAASLMKIANDIRWLASGPRCGIGEIIIPENEPGSSIMPGKVNPTQSEAMTM
                     VASQVIANDVAVNIGGASGNFELNVFKPLIIFNVLNSIRLLSDACTMFEEHCAVGIEP
                     NKDKIEYNLHNSLMLVTALNPHIGYDNAAKIAKTAHHENTTLLEAGKKLGLLDEEKFK
                     AWVKPESMIKPGL"
BASE COUNT          910 a          484 c          472 g          731 t
ORIGIN      
        1 gtctgtctcc actctacttg ttccagctca cctagtcccc tttctaaaat ctaaatctct
       61 ttctttcgga agtcttaaca catacttcaa atttctactc tcccaatatc gcatcatcac
      121 tcattccggt ctcattccct atcctgcttc catcaaaacc caatttcaaa aaaataattt
      181 ggggctaaca cgtgttagct ttcgtgtaga taacgatgat tggatagaac ttggtgaact
      241 tagtatcgct tttgggaaat ctcgctgttg gttgtttact tatttgttag agttagactt
      301 tttaggttta tgggaaattc tatctaaaac cgacttgatc aacgaagttc ctacggtcga
      361 taggctagga ctgagggctt cttggtcttt cagaaggact gactacatct acaagcgctc
      421 ctactatgtg agaatttagt atagaaacta aatagccaag aaatagaatc aaatcaattc
      481 atacaaacaa ataagaatgg gtaatctatg cccagagaat ctttcaattt tctacttgaa
      541 ataaattcta cacaaagcga tgagtagaat atgcaaaata tatttttatt tctccttttt
      601 ttagttatcc agtgtagttc tgttcaatat gtaaaaaaaa atccaactct aaaggatgat
      661 atctctagca caaaacgaat tgcaatagtt tccgccaaag acagtaatat atcaaaaaat
      721 gaatacttta tgttacacga catgctacgt caggaaattt ctcatcatac tttatttata
      781 gtttataaag cacctacaaa attcaagaat atctgttcaa aggaatttcc aaaggttgag
      841 ggggttttga tcgcggagat gaaggaatat tcagaagcca gtaagtccaa atttgaatta
      901 aaggcagcgc ttagaaaatg ttctactgat tcaacaatct ggagcgcaaa ggttgcagac
      961 aaattctctc ttgaagcaaa taaagagcta tctcttgtaa agggttatgt tggaaagtat
     1021 ggtgattcag ttgcaaacaa agtcaatgcc ttcttccaac tttctaaaga tttagtttca
     1081 gaactacaag gtcctgaaaa attaagtgaa gaagaagagt tagaaaaaat cgaagtagaa
     1141 tcagaataaa gcgataggcg catttcgcaa ttatatttaa cacaaataat aaatgaagga
     1201 taaacaaatg gcaacaagaa aagaaacaga ttcaatggga gaaatcctag ttgatgattc
     1261 aaaatattgg ggagctcaaa cagagcgttc cttacatcat tttaaaattg gcacagacaa
     1321 atttcctaga gaaatgattc gggcacttgg tgttctaaaa aaatctgctg caatcgtaaa
     1381 tgcagaactt ggaattttgc cagcagacaa aagagactta atcgtaaaag tggcagatga
     1441 agtaatttcc ggagtattag acgagcattt tccacttagt atctggcaaa caggttcagg
     1501 aactcagact aatatgaatg ccaatgaagt aatttccaac cgcgcaattg aattagcggg
     1561 cggaataaaa ggatccaaaa aaccaattca tccaaacgat gatgtaaaca aagctcaatc
     1621 gtctaacgat acctttccta cggctatgca cattgcagcc gcagaacaat taaataagag
     1681 actcatccca acacttacta agctacggga cacactaaaa aagaaatcag aagacttcaa
     1741 agatattatt aaaatcggaa gaactcattt acaggatgca actccattaa cattaggaca
     1801 agaattttct ggttatgttc aacaacttac atatggaatt gaacgggtta aacatgtctt
     1861 accttccgtc tatcgacttg caattggagg aacagcagta ggaaccggtt taaatacgca
     1921 tccagaattt gccgaaaaag ttgcaaagca aattgcaaaa gaaacaggtc taccttttgt
     1981 cagtgccgaa aacaaattcg aggcgctagc tgctcatgat tccctcgtag aagccagtgg
     2041 cgttttaaaa acgttagccg catctcttat gaaaattgca aatgatattc gctggctagc
     2101 atcaggtcct cgctgtggaa ttggagaaat catcatacct gaaaacgaac caggctcttc
     2161 tatcatgcca ggaaaagtaa atccaactca atcagaggct atgacaatgg ttgcttcgca
     2221 agtgattgca aatgatgttg cagtaaatat cggtggcgca tctggaaatt ttgaattgaa
     2281 cgtctttaag ccactcatta tcttcaatgt attaaatagc attcgccttt tatcggatgc
     2341 ttgcactatg tttgaagaac actgtgctgt aggaattgaa ccaaataaag ataagattga
     2401 gtataatctt cacaattctc tcatgcttgt tacagcactt aaccctcata ttggctatga
     2461 caatgcagct aaaattgcta agacagctca ccatgagaac acaacacttc tagaagctgg
     2521 aaaaaaatta ggacttctag atgaagaaaa attcaaagca tgggtaaaac cagaaagtat
     2581 gatcaaacct ggtttat
//
LOCUS       DAOQOS010000270         2596 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_32221_length_2596_cov_6.142464, whole genome shotgun sequence.
ACCESSION   DAOQOS010000270 DAOQOS010000000
VERSION     DAOQOS010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2596)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2596)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2596
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32221_length_2596_cov_6.142464"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(19..354)
                     /locus_tag="PLF66_26420"
     CDS             complement(19..354)
                     /locus_tag="PLF66_26420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67649.1"
                     /translation="MKSRNFHVILFSLFIGILYFLILFSNLQRQLDHDQIVYTNNIRS
                     FFVEKQFVNPHHLHFEPTGFYFHKFLLSNFSDYLGTNLIFHLRIRSLLVASIGISLCF
                     LYLFWKQLN"
     gene            complement(351..935)
                     /locus_tag="PLF66_26425"
     CDS             complement(351..935)
                     /locus_tag="PLF66_26425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HMZ67650.1"
                     /translation="MLSGIKLRYKSYLENRIRNWTGLSSGEVNVYRSNRFVLFLFIFG
                     FTLLCLYCYSFYPQFSKLLNDGFLFFHFPKIFNLKTPEPIFFERLAQYFLGGFLLFHW
                     IRIEAFLLEYFFSCLYISKEQKMIIYIESNLFKRKIHILKNKRMQYELEDNLILRIFG
                     IGSLKISNENQVWEIRYLDKAKYALTEIISFDKK"
     gene            complement(1065..>2596)
                     /locus_tag="PLF66_26430"
     CDS             complement(1065..>2596)
                     /locus_tag="PLF66_26430"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020770960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="V-type H(+)-translocating pyrophosphatase"
                     /protein_id="HMZ67651.1"
                     /translation="GGGIYTKAADVGADLVGKVEKGIPEDDPRNPATIADNVGDNVGD
                     IAGMGADLFGSAAEATCAALVIGATATALSGNTNALLYPVLISAFGIPACLITSFFAR
                     VKEGGSVEGALKGQLWISTFIVAIIMYFVTDRLMVDSFEIAGKTITKMNVYSSLVLGL
                     FSGMFIGVITEYFTSHSYKPVREVADSCNTGAATNIIYGLALGYKSSVLPVVLLVITI
                     VGSNVLAGMYGIAIAALGMISTIAIGLTIDAYGPVSDNAGGIAEMAELGKEVRNRTDT
                     LDAAGNTTAAVGKGFAIGSAALTSLALFAAFITRSRLQDSTIGNIELLDPLVFGGLLF
                     GAMLPFIFSAFTMKSVGDAAKDMVEEVRRQFREIPGLMEGKNKPDYKKCVDISTTAAL
                     REMIAPGLLVILSPLVVGYLFGIKCLAGMLAGALVSGVVLAISSANSGGAWDNAKKYI
                     EAAGKKGTDLHKAAVVGDTVGDPFKDTSGPAINILIKLMAIISLVFVEFFVSHGGLLV
                     K"
BASE COUNT          949 a          481 c          415 g          751 t
ORIGIN      
        1 tcacggagta agtggcgttt aattgagttg tttccaaaat agataaagaa aacagagaga
       61 gattccaatt gatgcaacaa gcagagatcg aatccttaaa tgaaaaatta gattcgttcc
      121 aagatagtca gaaaaattcg aaagtaaaaa tttgtggaaa taaaatcctg taggttcaaa
      181 atgcaaatga tgtggattta caaattgctt ctccacaaaa aaagatcgaa tattatttgt
      241 ataaacaatt tgatcatgat caagttgtct ttgtaaattg gagaataaaa ttaaaaaata
      301 taaaattccg ataaataaag aaaaaagaat tacatgaaaa tttctagatt tcatttttta
      361 tcaaaggaga ttatttcagt taacgcatat tttgccttat caagataacg aatttcccag
      421 acttggtttt cattggaaat ctttaaacta ccaattccaa agattctaag aattaaatta
      481 tcttctaatt catattgcat tcttttattt tttaaaatat gaatctttct tttaaataga
      541 ttactctcga tatatataat cattttttgt tcttttgaaa tgtataagca ggaaaaaaaa
      601 tattccaata aaaatgcttc tattcgaatc caatgaaaga gtagaaatcc tcctaaaaaa
      661 tactgagcca gtctttcaaa aaaaattggc tctggggttt ttaaattaaa aattttagga
      721 aagtgaaaaa ataaaaatcc atcattcaga agtttagaga actgaggata gaaagaatag
      781 caataaaggc aaagtaaagt aaaaccaaat ataaatagaa ataaaacaaa tcgatttgat
      841 cggtagacat ttacttctcc agaagaaaga cctgtccaat tgcggattct attctctaaa
      901 taagatttat atcttaattt tattcctgaa agcatatcag actaagatag actgaaatct
      961 aattttatcg actgaaaaat atttttttta agaaaaggga aaaaagaaat tgggaaagtt
     1021 cacagtattt ctataaacac tgtgaactta ttaagagatg tgaattattt aactagaaga
     1081 ccaccatggc ttacaaaaaa ctcaacaaac actaggctga taattgccat gagtttgatt
     1141 agaatattga tagcaggacc agatgtatct ttgaacggat cacctactgt gtctcctaca
     1201 acggcagctt tatgaagatc agttcctttt ttaccggcag cttcaatgta tttctttgca
     1261 ttgtcccaag ctccacctga gtttgcactt gaaatagcaa gaaccactcc agaaactaac
     1321 gcacctgcaa gcataccagc aagacactta attccaaata aataaccaac aactaatgga
     1381 gaaaggatca ctaagagccc cggagcaatc atctcacgaa gagctgcggt agtagaaata
     1441 tccacgcatt ttttgtagtc aggtttattc tttccttcca taagaccagg aatttctcta
     1501 aactgacgtc ttacttcttc caccatatct ttggcagcat ccccaacaga tttcattgta
     1561 aatgcagaga aaataaatgg aagcattgct ccaaataaca atcccccaaa tacgagtgga
     1621 tcgagtagtt caatatttcc aatggtagaa tcttgtaaac gagaacgagt aataaaagct
     1681 gcaaaaagag caagtgaagt aagagcagcg gaaccgattg caaaaccttt tccaactgca
     1741 gcagttgtat ttcctgcagc atctaaagta tctgttctat tcctaacttc ttttcctaat
     1801 tcagccatct cagctatacc accagcgtta tccgaaacag gaccataggc atcaattgta
     1861 agaccaattg caatggttga aatcattccg agtgcagcga ttgcaattcc atacattccc
     1921 gctaatacat ttgaaccaac aattgtaatt actaataata ctactggtag aacagaactt
     1981 ttatatccga gtgctaaacc atagatgatg tttgtagcag cccctgtgtt acaagaatct
     2041 gcaacttctc taactggttt gtaagaatga gaagtaaaat actcagtgat aacaccaata
     2101 aacattccag aaaaaagtcc aagaactaga gaagaatata cattcatttt agtaattgtt
     2161 tttcctgcga tctcgaaaga atctaccatg agtctatcgg ttacaaaata catgatgatc
     2221 gcaacgataa aggtagaaat ccaaagttgt ccttttaaag caccttctac actaccgcct
     2281 tctttcactc ttgcaaagaa gctagtaatt aaacaagctg gaataccaaa tgcagaaatt
     2341 aaaacagggt ataaaagagc gtttgtattt ccactgagtg cagtcgctgt tgctccaatc
     2401 acaagagcag cgcaagttgc ttcggcagca gatccaaaaa ggtcggcacc cataccagca
     2461 atatcaccca cgttatcacc aacgttatcc gcaatagttg cagggtttct aggatcatct
     2521 tcagggattc ctttttctac tttacccact aagtcggcac caacgtcagc agcttttgta
     2581 taaataccac cgccca
//
LOCUS       DAOQOS010000271         2550 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_33292_length_2550_cov_5.426854, whole genome shotgun sequence.
ACCESSION   DAOQOS010000271 DAOQOS010000000
VERSION     DAOQOS010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2550)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2550)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2550
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33292_length_2550_cov_5.426854"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            351..1016
                     /locus_tag="PLF66_26435"
     CDS             351..1016
                     /locus_tag="PLF66_26435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67652.1"
                     /translation="MLGITTSGILAVAVSGTFNTFTTGSLLKSADINANFASLKTAIE
                     GIPTQKAMRLIYEYDVPSATTSVNVTGLDGDTDLTYDVRARFVSGTAVGGGYAYYIRP
                     NNDSSSSYFSRFTYIDNAATPTKSYSGSIDNLGMNLCGSTMTITTSSVCFGNITIFAK
                     SGYARMSAGKGFHHDAAANWFNYDYSTTYTNTGTNITSLTIYSPTSNAIGAGSRIEVW
                     ARR"
     gene            1061..2287
                     /locus_tag="PLF66_26440"
     CDS             1061..2287
                     /locus_tag="PLF66_26440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67653.1"
                     /translation="MKLKILIVLFLFVGKSLLADEVVLKNGKVYENVKANLLRSVIQF
                     TFEEKLLTFKKSDVKRIRLRPVILKPPTNEVERLAYEAERIRVAEALQNNTDWEMTSN
                     SKQSVAVIKLLPGSGVSLAEVESVTNLIRTSLVKTKLFVIIDTTSLESSAKQSECPPN
                     KKDCAPKLPENVKVNKLVTGTLTRLGNKYLINGTVLDGKENKVVFAEKAAATSQDKLE
                     EASEYFAKKVAGGLMEDWNEALTAKENETYANLQYVWRSSLIPGLGQWKYGKDKEDSS
                     SVKKGFAFGAVTAVLLINVIYHVQKLSSARESYDSNHRFFFLTPSGSGLELIALNADN
                     QSFANWQNVSNTTKLSAYALIGFYLYNVIDAFFLGKPFFSRKESTSGIFILPSKYTYS
                     NGLRENYYTIGMQFQF"
     gene            2299..>2550
                     /locus_tag="PLF66_26445"
     CDS             2299..>2550
                     /locus_tag="PLF66_26445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67654.1"
                     /translation="MRKIAFKIFLFFNFLLFANCGIFTDPDRGVTPYSKFLSVLFSDR
                     SPATVTSVLPKDKSTGNFINTNLFVVFNKTVSGFNTTNFT"
BASE COUNT          848 a          429 c          481 g          792 t
ORIGIN      
        1 cagacttctc cgataggaat cgttctttct gaatggggaa ggtattagaa ttttgaatca
       61 gtatcatata gaataagagg aaggaaatcg ttgttatgtg cgcggaaatt gaaaaaaaaa
      121 tgcgataact ttgatttacc tcaagaaatc ttatggtaaa gagattgaca atgagagcag
      181 gtcgcagaat ttgagtagaa ctaaaatctg tgtgcgagga tactatggat ttgcccaaca
      241 aatcatttaa ggaaccaaca tcattttata taattaaaaa cttttttaca atatctaaaa
      301 aattcttagg tgagaactta tttaaaggat tcatgcaagg tgtcggaatt atgttgggca
      361 ttaccactag cggcatatta gcagtagccg ttagtggaac attcaataca tttacaaccg
      421 gctctctctt gaagtctgct gacatcaatg caaactttgc atccctaaaa acggctattg
      481 aaggaattcc gacgcagaag gcgatgagat tgatatatga atatgatgtt ccgagcgcga
      541 caacatctgt caatgtaacc ggattggatg gagatactga tttaacatat gatgttagag
      601 caagattcgt tagtggaaca gctgttggtg gaggatatgc ttattatatt aggccaaaca
      661 atgattcttc ttctagttat tttagtagat ttacctatat tgataatgcg gctactccaa
      721 caaagtcgta ttcaggatcc attgataacc ttggaatgaa tttatgcgga agcacaatga
      781 ctataacaac tagtagtgtt tgttttggta atataacaat ttttgcgaaa tccggatatg
      841 caagaatgtc tgctggaaaa ggatttcatc atgatgctgc tgccaattgg tttaattatg
      901 attattcgac aacgtataca aatactggaa cgaatattac ttctttaaca atatattctc
      961 caacatcaaa tgcaattggt gccggatccc gcatcgaagt ctgggcaagg cgatgagttc
     1021 taacttaaaa cgaaaagaat tttcttaaag gagaaaccat ttgaaattaa aaatactgat
     1081 agtccttttt ctgttcgtag gcaaatccct attagccgat gaagttgtct taaaaaatgg
     1141 aaaggtttac gaaaatgtaa aagcaaactt gcttaggagt gttattcagt ttacctttga
     1201 agaaaaactt ctaacattca agaaatcgga tgtcaaaagg atacgactac gacctgtaat
     1261 tttaaaacca cccactaatg aagtggaaag acttgcctat gaagcggaac gtatccgcgt
     1321 tgccgaagct ctacagaata acacagattg ggaaatgacg tctaacagta aacagtcagt
     1381 agccgttatt aaacttctcc cgggaagtgg agtgagtctc gctgaagtag aaagtgtcac
     1441 aaatttaatt cgcacaagtc ttgtaaaaac taaattattt gtcatcattg atacaacatc
     1501 attagaaagc tctgcgaaac agtcggaatg tccccccaat aaaaaagact gtgctcctaa
     1561 gcttcctgag aatgtaaaag taaataaact tgtcactgga actctcactc gactcgggaa
     1621 taaatatctc atcaatggaa ccgttttaga tggtaaagaa aataaagttg tctttgctga
     1681 gaaagctgcc gctacttcac aagataaatt agaagaagca tccgaatact ttgcaaaaaa
     1741 agtagcaggg ggcttgatgg aagattggaa tgaggcttta actgcaaaag aaaatgagac
     1801 atacgcaaat cttcaatatg tgtggcgttc ttctcttata cctgggcttg gtcaatggaa
     1861 atacgggaaa gataaagaag actcttctag tgtaaaaaaa ggttttgcat ttggtgctgt
     1921 cactgcagtt cttctaataa acgttattta ccatgttcaa aaactttctt cggcacggga
     1981 atcctatgat tcaaatcata gattcttttt tcttacacct tcgggatcag gattagaact
     2041 gattgctctt aatgcggata atcagagttt tgcgaactgg caaaatgtat ctaatacgac
     2101 aaaactatct gcttatgctc taattggatt ttatttatat aatgtcattg atgctttctt
     2161 cttaggaaag cctttctttt ctagaaaaga atcaactagc ggtatattca tattaccctc
     2221 taaatacact tattccaatg gtcttagaga aaattattac acaataggaa tgcaatttca
     2281 attttaagga gactcttcat gagaaaaatc gcttttaaaa tatttctttt ttttaatttt
     2341 cttctatttg ccaattgtgg tatttttaca gatccggatc gaggggttac tccttattca
     2401 aaattccttt ctgtattatt ttccgatcgg tcacctgcaa cggttacatc ggttttacca
     2461 aaagataaat ccactggaaa tttcattaat acaaacttat ttgtcgtctt taataagact
     2521 gtttctggat ttaacactac taattttact
//
LOCUS       DAOQOS010000272         2517 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_34073_length_2517_cov_5.627132, whole genome shotgun sequence.
ACCESSION   DAOQOS010000272 DAOQOS010000000
VERSION     DAOQOS010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2517
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34073_length_2517_cov_5.627132"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1133
                     /locus_tag="PLF66_26450"
     CDS             <1..1133
                     /locus_tag="PLF66_26450"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HMZ67655.1"
                     /translation="FGERKFGDKKFEDRKFGDRKFDDKKFEDRKFGDRKFGDNRFKED
                     RYEDRKFSRSRFEDRDFEEEEEAPELPPEKREVRRSKLDPSEWKQKSLPKTSKKLKVK
                     KEKTETILEQTEEEKLELLSELREESFGGDARNFSDGEPEKIFRINRFLAKSGLGARR
                     DVEALIKDGKIKVNGEVETSLSRKIDTEKDIVEYENEKVELISDKTILAFNKPEGYLC
                     SHHDVHHEKTVFNLLPLKYRKFNMAGRLDLTSRGLMIFSADGETLNQISHPSYAIKKR
                     YIVKLDKVIQESEFVAAFLKGVEDEGELLRAKEVSVIDSENAMISISLQEGKKRQIHR
                     MFKAIGYKVLDLQRVQIGKLLLEDLNLPEGKFIEITREDIFG"
     gene            1139..>2517
                     /locus_tag="PLF66_26455"
     CDS             1139..>2517
                     /locus_tag="PLF66_26455"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016548052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HMZ67656.1"
                     /translation="MSFLTIKFFTLFLIVFYIYWNVGARAKRALILIASCIFYSFFSF
                     NFLLHFLFIIFVNYLFIRYLREKDSFLKISIGFNLANLFLFKYFYFFLETIGYISGIE
                     ILTHKTLLNQYFSNLTHFANFEVVLPATISYYTFQLISLSVDLKKGEIHELISFYDVA
                     SYVLFFPVMIAGPITRFKDIVPKMEEPILSREDMREGIWKILLGITKKVALSDSITTI
                     IYPVFKEPENYSGLSILLTSYFFAIHLYLDFSGLTDIARGLGRLLGYNLPENFKAPFL
                     LNSFSDFWRRWHLSFSFWIRDYIYIPLGGSRVSEWRGYFNLLITFILGGLWHGASMNF
                     MMWGFLTGLFLSLERFFELKQIKILPENKFKPFIKYLFVLHVYMISWILFFTTSLKAG
                     FTVIWRVLTMKDGFPIGNLELGIYVTIFGMLFQLSEEYPEKFQWIQKIKPALLPIFSF
                     IIVLLILNN"
BASE COUNT          852 a          366 c          467 g          832 t
ORIGIN      
        1 aattcggaga gcgcaaattc ggcgataaga agttcgaaga tagaaaattc ggagaccgca
       61 aattcgacga taagaagttt gaagatagaa aatttggaga ccgtaaattt ggcgacaatc
      121 gattcaaaga ggatcgttat gaggacagaa aatttagtag aagtcgcttt gaagatagag
      181 attttgagga agaggaagaa gctccagagc ttccccctga aaaaagagaa gtaagaagat
      241 ccaagttaga tccgagtgaa tggaagcaaa aatctctacc aaagacatct aaaaagttaa
      301 aagtcaaaaa agaaaaaaca gaaacaattt tagagcaaac agaagaagaa aaattagagt
      361 tactttctga attaagagag gaatcttttg gcggggatgc aagaaatttt tcggatggag
      421 agcctgagaa gatatttcgg attaaccgct tcttagcaaa atcaggactt ggagctagaa
      481 gagatgtgga agcactcatt aaagatggaa agataaaagt aaatggtgaa gtagaaacat
      541 cgctatctag aaaaatcgat acagaaaaag acatcgttga atatgagaac gagaaagttg
      601 aactgatctc agataaaaca attttagcct ttaataagcc tgaaggatat ctttgttctc
      661 atcatgatgt gcatcacgaa aaaactgttt ttaaccttct accactcaaa tatagaaaat
      721 ttaatatggc tggtagactc gatttaactt ctaggggact aatgatattt tccgccgatg
      781 gagaaacttt aaatcaaatc tctcaccctt cctatgcgat taagaaaaga tacattgtga
      841 aattagacaa ggtcattcaa gaatccgaat ttgtagcagc gtttcttaaa ggagtagaag
      901 acgaagggga actactgaga gcgaaagaag taagcgtgat cgattctgaa aacgcaatga
      961 tttctatctc cctgcaagaa ggaaagaaaa gacaaattca cagaatgttt aaagcaattg
     1021 gttacaaagt tttagattta caaagagttc agattggtaa attactttta gaagatttga
     1081 atttaccaga aggtaagttt atagaaataa ctagagaaga tatttttgga taattcaaat
     1141 gagtttttta acgatcaagt tttttacact ctttctgatt gtattttata tctattggaa
     1201 tgtaggagct agggcaaaaa gagcattaat tctaattgcc tcctgcatct tttacagttt
     1261 ctttagtttt aattttcttt tacatttttt atttatcatt tttgtaaact atttgtttat
     1321 acgatattta cgagaaaaag attcttttct taaaatctca attggtttta atctagccaa
     1381 tttatttctt tttaaatatt tttatttctt tttagaaacg atagggtata taagtgggat
     1441 tgaaatactt acccataaaa cccttctaaa ccaatacttc agtaatctca cacattttgc
     1501 aaattttgaa gtcgttttac cggcgacgat cagttactat actttccagc ttatttctct
     1561 ttctgtagat ttaaaaaaag gagaaattca cgaattaata tctttctatg atgtcgcttc
     1621 ctatgtgtta ttttttccgg tgatgattgc aggtcccatc actcgtttta aagatatcgt
     1681 tcccaaaatg gaagagccaa tactttctag agaggatatg cgcgaaggga tttggaaaat
     1741 actcttagga attacaaaga aggtagctct ttctgattct attacaacta tcatctatcc
     1801 tgtttttaaa gagccagaaa actattcagg tctttcgatt ttactcacca gctatttttt
     1861 tgcaatccat ctctatttag atttttcggg gctaacagac attgcccgcg gattgggaag
     1921 gttacttgga tataacctac cagaaaactt taaagctcct tttttattaa atagcttttc
     1981 cgatttttgg aggagatggc atttaagttt ttcattttgg atcagagatt atatttatat
     2041 tccgctaggc gggtctagag tgagtgaatg gagaggatat tttaatttac tgattacatt
     2101 tatactaggt gggctttggc atggggctag tatgaatttt atgatgtggg gttttttaac
     2161 tggacttttt ctttccctcg aaagattttt tgagttaaaa caaatcaaga ttctacctga
     2221 aaataaattc aaaccattta tcaaatatct atttgtatta catgtttata tgatttcttg
     2281 gatattattt tttactacct ctttaaaagc tgggtttact gtgatttggc gtgttctgac
     2341 tatgaaagat ggatttccaa ttgggaatct agaattaggt atctatgtta ctatatttgg
     2401 aatgctcttt caactatcgg aagagtatcc agagaagttt caatggattc aaaaaataaa
     2461 accagctcta cttccaatat tttcttttat catagttcta cttattttaa ataacag
//
LOCUS       DAOQOS010000273         2508 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_34319_length_2508_cov_5.668161, whole genome shotgun sequence.
ACCESSION   DAOQOS010000273 DAOQOS010000000
VERSION     DAOQOS010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2508)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2508)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2508
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34319_length_2508_cov_5.668161"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..510)
                     /locus_tag="PLF66_26460"
     CDS             complement(<1..510)
                     /locus_tag="PLF66_26460"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HMZ67657.1"
                     /translation="MNLSKLFSRADDLTIQEIIGYTPVSLLQKVMPEKATPSQLKKLV
                     VELFHWEGLLLKDDTRKKIIELLREEEIQDLFIKLGLKSNGNKYEVINKIKFERGSDR
                     ERILFDFFEISVPEVEIKKILKAFEEAEPPYPLFPHQRKAIQQMEERLTTNAKRIILH
                     MPTGSGKTRT"
     gene            complement(507..1340)
                     /locus_tag="PLF66_26465"
     CDS             complement(507..1340)
                     /locus_tag="PLF66_26465"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HMZ67658.1"
                     /translation="MIDLYFDYQKKNVFKIPDSISLERNSYEISDRISNGGNAVVHKC
                     FDSIGNEYAIKFFLKTEPVKKKRFLQEIEVMEKIDHHHIVKVLDSGIVSVQNKQVSEA
                     PCIVMELADSNLQMLKNQKEIPYFDYIEQFRGLSSALAELHKLGIHRDIKPDNILIFG
                     DRWVLSDFGLFKYNEPNNLNLTNTDEIPNPKTWLSPEGINSYYSTDSISHASDVFQMG
                     CIFWYVVNGKYPLGVVEKDDWKGKESLFEFMIKTLFHNVKKRISNGTEMLQYLEDSLI
                     A"
     gene            complement(1337..>2508)
                     /locus_tag="PLF66_26470"
     CDS             complement(1337..>2508)
                     /locus_tag="PLF66_26470"
                     /inference="COORDINATES: protein motif:HMM:NF015210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HMZ67659.1"
                     /translation="RDWDDNKLLGISPEYQRREVWTDAAKVMLIDTIIKNIPIPKIYI
                     STTIKNGKSFRRVIDGQQRIRSILSFVRNEFSLKKPYDNDSFKNNKFSDLPEEIQDNI
                     LTYLIDFNEIYNTTVDIERDIYLRVNKYTKVLNKQELRRADYPGEFLSLSEQLSFVEF
                     FKEANIFTYAQVRRMLDIEFISELLAIQLEGIADKKEGLEKCYEYYSDMDKKRSAELK
                     EEFFKVIQEIEIIFNHDPVKLSKTRFKQKADLYTLFSAILELNRKGLFITNKDCSFLL
                     ADIQLLDKHIAPESEISLLSEYAIKCTSQANSNSSRVYRKNFMLHFLKGTYISEFPNE
                     ETIRTFKKIKYEIFKYIENRNISDNIDSYKISWSPNTTQYQLSNSILIDEKGNVV"
BASE COUNT          795 a          441 c          310 g          962 t
ORIGIN      
        1 cgttctagtt tttccagaac cggtcggcat atgtaatata attcgctttg cgtttgtcgt
       61 tagtcgctct tccatttgtt gtatagcttt tctttgatgt ggaaacaatg gatagggagg
      121 ctccgcttcc tcgaaagcct ttaaaatttt ttttatttct acttccggaa cagaaatttc
      181 aaagaaatca aataatattc tttctctgtc tgaccctctt tcaaatttaa ttttatttat
      241 aacttcatat ttattaccat ttgatttcaa tcccagtttg ataaacaaat cttgaatttc
      301 ttcttctcta agtagttcta tgattttttt tctagtatca tcttttaata aaagtccctc
      361 ccaatgaaat aactcaacta caagtttttt caactgagaa ggagttgcct tttctggcat
      421 gactttctgc aagaggctaa caggcgtata cccgataatt tcttgtattg ttaaatcatc
      481 cgctcgtgag aatagtttgg ataggttcat gctattaaag aatcttccaa atattgtagc
      541 atttcagtgc cattgcttat tctcttcttt acattgtgaa acaatgtttt aatcatgaat
      601 tcaaataaac tctctttccc tttccaatca tctttctcta ctactcctaa tggatacttt
      661 ccattaacta cataccaaaa aatacacccc atctgaaata cgtcagatgc gtgacttatc
      721 gaatcagttg aataatatga attaattccc tctggagaaa gccaagtttt cggattaggg
      781 atttcatctg tattagttag atttaaatta ttcggctcat tatatttaaa tagtccaaaa
      841 tcactcaaca cccacctatc accaaagatt aatatattat ctggttttat atcacgatga
      901 atacctaatt tatgcaattc agccaaagca gaggaaagac ctctaaattg ctcgatatag
      961 tcaaaatatg gaatttcttt ttggttcttt aacatttgta aatttgaatc tgctaactcc
     1021 attactatac aaggggcttc cgatacttgc ttattttgaa cagaaacaat accactgtct
     1081 aataccttta caatatggtg gtgatctatt ttttccataa cctcaatttc ctgaagaaat
     1141 cttttctttt tgacaggctc agtttttaaa aaaaatttta ttgcatactc attaccgatt
     1201 gaatcaaaac atttatgcac aactgcattt ccaccattgg aaattctatc actaatttca
     1261 taggaattac gctctagtga tattgaatca ggaattttaa aaacattctt tttctgataa
     1321 tcaaaatata gatctatcat acaacgttcc ctttttcatc tattaaaata gaattagata
     1381 attgatattg agtagtattt ggtgaccaac ttattttgta tgaatctata ttatcagaaa
     1441 tatttctatt ttcgatatat ttaaatattt catatttaat ttttttaaat gttcttatag
     1501 tttcttcatt tggaaattca gagatatagg ttccctttaa aaaatgaagc ataaagtttt
     1561 ttctatacac ccgactacta ttagaattag cttgtgaagt acattttata gcatactccg
     1621 aaagcaaact tatttcagat tctggagcaa tatgcttatc gagtaattga atgtcagcta
     1681 aaagaaaact acaatcttta tttgttataa ataaaccttt tctattcaac tcaagtattg
     1741 cactgaataa agtatataaa tctgctttct gtttaaatct agtttttgat aacttaacag
     1801 gatcatgatt aaaaataatt tcaatctcct gaattacttt aaagaattct tctttaagtt
     1861 cagcagatcg ttttttatcc atatcagaat aatattcata acacttttct aatccttctt
     1921 tcttatcagc aatcccttcc aactgtattg ctaatagctc agaaataaat tcaatgtcga
     1981 gcattcttct tacttgtgcg tatgtaaaaa tgttagcctc cttaaagaat tcaacaaaag
     2041 ataactgctc tgaaagactt aaaaactcac ctggataatc agccctacgt aattcttgtt
     2101 tgttaagaac cttagtatat ttatttactc tcagatatat atctctttca atgtcgactg
     2161 tcgtattata tatttcatta aaatcaatta ggtatgttaa aatattatct tggatttctt
     2221 caggcaaatc agaaaattta ttatttttaa aagaatcatt gtcataaggt ttttttaaag
     2281 aaaattcatt tctaacaaaa gatagtatcg accttattct ttgttgccca tcaataaccc
     2341 ttcgaaaaga ctttccattt tttattgtag tactaatata tatttttgga atcggtatat
     2401 tcttaatgat cgtatcaatc aacattactt tagctgcatc agtccatact tctctacgtt
     2461 gatattctgg acttattcca agtagtttat tgtcatccca gtctctta
//
LOCUS       DAOQOS010000274         2322 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_39550_length_2322_cov_4.641817, whole genome shotgun sequence.
ACCESSION   DAOQOS010000274 DAOQOS010000000
VERSION     DAOQOS010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2322)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2322)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2322
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39550_length_2322_cov_4.641817"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(257..859)
                     /locus_tag="PLF66_26475"
     CDS             complement(257..859)
                     /locus_tag="PLF66_26475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67660.1"
                     /translation="MKQKIIVIMLVLFILPIYSFEIVLENGDAFICDLITETDKSYKI
                     FYKDKEYTIPKTEVTSTDSTKKGAHSAFRYAKFTLKDGSKLNGVIAEETNDSYTLKTE
                     LGFLVVEKRRIAEFPPKTRTNPEIPSTYLASNVRLPETRIGFFGSGYVNGKPISDTNP
                     TSGGGGLYVEPAFLQWKFLRFGFRTEYLQSYGKSWTQVSR"
     gene            complement(1144..1404)
                     /locus_tag="PLF66_26480"
     CDS             complement(1144..1404)
                     /locus_tag="PLF66_26480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67661.1"
                     /translation="MKKKIILIFVFIISCSDSITERQKCKEDVLFRDEMVMDPETCII
                     LLSAAGGRNDVAANSGRDLVLVSCLKYTYRMWKCAKKSDILP"
     gene            complement(1422..>2322)
                     /locus_tag="PLF66_26485"
     CDS             complement(1422..>2322)
                     /locus_tag="PLF66_26485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67662.1"
                     /translation="LVSFWKPKIVSFFYVGVHNLDDIHGMNHYMYVNGSPLNFRDDSG
                     NSCSKNYSGQITGAIGSAIGSYYGGMYGGPTGASNGTLAGYSTGVAAFGVAPGHNWTG
                     GGGSCSKRSPNEPLALVALFSGQLLMSQNNPIELFAKFILLKQYYEMQKSRGESSFVD
                     GFVTDRKSYTMALIGLVLTQQLDSFYAAMLFILMTRNVVQPSSLSKGIDTASYQHDTS
                     RGANVLGFSGHDPRAASSRWIKSAWKGIHNPYDAWVAGTGIVLFSLANAIYSATHINR
                     VKYDIKPARTRSIRPCAGGCNFM"
BASE COUNT          707 a          479 c          355 g          781 t
ORIGIN      
        1 tattttttat taaaatctat tctgtaaacc ctttttctta tttcgcaaca gactgcagat
       61 tcattcaaag agcaatatcc tgatatacct tgggatgaaa tgcgcggaat gagaaattga
      121 ttcattcatg gttacaatag aattaaccta ggtgtatttt atagaacctg tgtagacgat
      181 ttatccactc tcctaattga gttgaacaaa ctataaaaaa acaaaaaaaa atttagaaac
      241 attatatttc tagcgcttat cgactcactt gtgtccaact ttttccgtag gattgtaagt
      301 attccgttct aaatccaaat cggagaaatt tccattgcag aaaagctggc tctacgtaaa
      361 gcccacctcc accagatgtt ggatttgtat ccgaaatcgg ttttccattt acataaccag
      421 atccaaagaa tccaattctt gtttcgggaa gtcgcacatt ggaagcaagg taggtagaag
      481 gaatttctgg atttgttcta gtctttggtg gaaactctgc aattcttctt ttctctacaa
      541 ctaaaaatcc aagttcagtc ttgagtgtat aactatcgtt cgtttcttct gcgatcactc
      601 catttagttt acttccatct ttcaaagtaa actttgcata acgaaaagca gagtgtgctc
      661 ctttctttgt cgagtcggta gacgtaactt cagtctttgg aattgtatat tctttgtctt
      721 tgtagaaaat tttatagctc ttatccgttt cggtgataag gtcacagata aatgcatctc
      781 cattctccag cactatttcg aatgaataga tgggaagaat gaaaaggact aacattatta
      841 ctattatttt ttgcttcata ctttctttgt ttcttgccta cttatttttt atatataacg
      901 ttttcctttt attcttctgt ttgattattg cttatcgttt tgtgcataat actgttcagt
      961 tatcagtgtc atattctttt gaaataataa aaaaagactt cgcttttcta tgtaatataa
     1021 tttatttcat tttcgattgc tcattaacaa tcttgtatca attcttgcat aactaagtcg
     1081 cgtcaatgaa gaacatcaag aaatacacat aatgaaatta acctttagca tctagtcaca
     1141 gtattagggt agaatatcag atttctttgc acatttccac attctatagg tatactttag
     1201 acaagacact aaaaccaaat cccttcctga attagccgct acatcgtttc ttccaccggc
     1261 tgcgcttaat agtataatac aggtttcagg gtccattacc atctcatccc taaacaagac
     1321 atcctcttta catttctgtc tttccgttat actgtcagag caagaaatta tgaatacgaa
     1381 tattaaaata atttttttct tcattaccat tctcctcaat attacataaa attacagcca
     1441 ccagcacaag gtcggataga tctagttctt gctggcttta tatcatactt tacacgattg
     1501 atatgagtag cggaatagat tgcattagct agagaaaata atactatacc tgttcctgct
     1561 acccaagcat cgtacggatt gtgaattccc ttccaagcac tttttatcca acgactgctt
     1621 gctgcccttg gatcatgtcc actaaacccc aagacattcg cccctctcga agtatcatgt
     1681 tgataagaag cagtatctat acctttgctt aacgaactag gttgcactac attacgcgtc
     1741 attaatataa atagcatagc ggcataaaaa gagtcaagtt gttgtgttaa gactaaacca
     1801 attaaagcca ttgtataact ttttctgtcc gttacgaaac catctacaaa ggaagactca
     1861 cctctagatt tttgcatttc ataatattgc ttaagtaaaa taaactttgc gaaaagttct
     1921 ataggattat tctgtgacat taaaagttgc ccactaaata aagcaactag ggcaagcggt
     1981 tcattcggac ttcttttact gcaactccca ccacctcctg tccagttgtg cccaggtgct
     2041 actccaaaag cagctacgcc tgttgaatag cctgccagcg ttccattcga tgcacctgtc
     2101 ggtcctccat acattcctcc ataataagaa cctatcgctg aacctatcgc accggttatt
     2161 tgaccagaat aattctttga acaggaattc cctgagtcat ctctaaaatt taagggacta
     2221 ccatttacat acatgtaatg attcattcca tgtatatcgt ctagattatg aacccctaca
     2281 taaaaaaagg acacgatttt aggcttccaa aaggagacta aa
//
LOCUS       DAOQOS010000275         2222 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_42760_length_2222_cov_3.744347, whole genome shotgun sequence.
ACCESSION   DAOQOS010000275 DAOQOS010000000
VERSION     DAOQOS010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2222
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42760_length_2222_cov_3.744347"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(90..383)
                     /locus_tag="PLF66_26490"
     CDS             complement(90..383)
                     /locus_tag="PLF66_26490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67663.1"
                     /translation="MINLNEGVGVTPLQEIVIQSMGAETRLINLYIDMINVIRDRNFV
                     YTDNRCKPESYEKISLEISSNRRTVANAIKKAKDKGDIDHLKKIAFRLLEAKI"
     gene            489..647
                     /locus_tag="PLF66_26495"
     CDS             489..647
                     /locus_tag="PLF66_26495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67664.1"
                     /translation="MTEKFVVPFFNGASIQIVKDEKNKPVFFDREVAEEIIKPVRDLI
                     KANDKRRG"
     gene            648..941
                     /locus_tag="PLF66_26500"
     CDS             648..941
                     /locus_tag="PLF66_26500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67665.1"
                     /translation="MRNPFTAKFWVIVFFAFMFLAGFGVSRILPQERFIPEKDSNEKP
                     FCHKEQFWLWDERQKGYWNQAYCHYKPLRQKDGFPRLFTAEEISEGIITKSKY"
     gene            complement(938..1372)
                     /locus_tag="PLF66_26505"
     CDS             complement(938..1372)
                     /locus_tag="PLF66_26505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67666.1"
                     /translation="MAGEAILENPDVITGCDAIVLFNGQLAGFIQDIDIEENVNQTPV
                     EAIGYWKPRGFKSLKWDGTFSCTFHTLAVTTQGVIKLDTSSPVAASAPYVFLFKQKST
                     GKRIASGIGHVNTKGFNLANNALSSRRVSFVLRDFKDLEGLN"
     gene            complement(1376..>2222)
                     /locus_tag="PLF66_26510"
     CDS             complement(1376..>2222)
                     /locus_tag="PLF66_26510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67667.1"
                     /translation="LCESVTGFYRNVLTSTFSVATYLADHCSKMVSPEGSNETFGGAG
                     AANTDAYATRLSNAKVLNSPWMCYGVNSLVTYQADGITEKTYDGWMLAVYHNAIKASA
                     NPRQAATNKVLNILRTDEELSPTQLINAEKSGCLTISKNPNGEYILLHGLTTYQGNNL
                     IYNLSQTIVTALALINDLRNSLWLEFGGRTPTDPDNLDKEFDDADIRTFVDNKFDIDY
                     IQNFGWLSRNIYTGEAAYDKNYTISRDGNVIYFNFPDGKVVSSIDYIFSLLNLDLIKG
                     SSTGV"
BASE COUNT          662 a          423 c          404 g          733 t
ORIGIN      
        1 cgcctaatgg catcaaagat aatttcattt ttcaaaactt ccttcaccta tccaatttgt
       61 tttgagcatt tcctttattt gttcgtcggt catattttag cctctaaaag cctgaaagca
      121 atttttttta agtggtcaat atcgccttta tcttttgctt ttttgattgc gttcgctact
      181 gttcttctat tgctagaaat ttccaaagaa atcttttcgt agctttctgg cttgcatcta
      241 ttgtctgtat acacaaaatt tctgtctctt atgacattta tcatgtcaat atagagattg
      301 ataagcctag tttctgcacc cattgactgg ataactattt cttgtagtgg agtaacgccc
      361 accccttcat ttagattaat catatttacc tctataatat gtatcggttt ttaataccat
      421 aactttagaa aaatacttct caagttattt tgcattgtat ccgagaatct ttttaggaag
      481 gtaaatttat gacagaaaaa tttgtagtgc cgtttttcaa cggtgcaagt attcaaatag
      541 taaaagatga aaagaacaaa cctgtttttt ttgacagaga agtagcagag gaaatcatta
      601 agccagttcg tgatttaatt aaagcgaatg acaaaaggag aggttaaatg agaaatccat
      661 ttacagctaa attttgggta attgtttttt ttgcgtttat gtttcttgca ggctttggag
      721 taagtcggat actccctcag gaaagattta ttcctgaaaa agattctaac gagaaaccgt
      781 tttgtcacaa ggaacaattt tggttatggg atgaaaggca aaaaggatac tggaatcagg
      841 cttattgcca ctataaacca cttcgtcaaa aagacggatt tccccgactt tttacagccg
      901 aggaaataag tgagggtatt ataactaagt caaaatatta attcaatccc tctaagtctt
      961 taaagtctct aagcacgaaa gacactcttc tagatgacaa agcattgtta gctagattaa
     1021 agccttttgt gtttacgtga cctattccag aagcaattcg ctttccagtc gatttttgtt
     1081 taaacaaaaa tacataaggc gcactagcag ccacagggct agaagtatcc aatttgatta
     1141 ctccctgagt cgtaaccgct agagtgtgga atgtgcaaga aaaagttcca tcccatttta
     1201 aagacttgaa cccgcgaggt ttccaatagc cgattgcttc cactggagtt tgattcacgt
     1261 tttcttcaat atcaatatcc tgtatgaatc ctgctagttg accattgaat aaaacaatcg
     1321 catcacagcc tgttattacg tccgggttct ctaatattgc ctctcctgcc atgccttata
     1381 ctcctgtgct agaaccttta ataaggtcca aatttaataa tgaaaaaatg tagtcgattg
     1441 aagaaacgac cttaccgtct gggaaattga aataaattac gttcccgtcc ctactgattg
     1501 tgtagttctt atcgtaggca gcttccccgg tgtaaatgtt tctagaaagc catccaaagt
     1561 tttgaatgta atcaatatcg aatttgttat ctacgaaagt tctaatatcg gcatcgtcaa
     1621 actctttgtc taagttgtct ggatctgtag gagtcctacc accaaattct aaccaaaggg
     1681 aatttctaag atcattaata agagccaaag cagttacaat cgtttgagaa agattgtaaa
     1741 tcaggttatt cccttggtag gtggtcaatc cgtggagcaa gatgtattcc ccgttaggat
     1801 ttttggagat tgtaaggcaa cccgattttt cagcattgat taactgagtt ggtgaaagct
     1861 cttcgtctgt tcttaaaatg ttcaagactt tgttagttgc tgcctgtcta ggattcgcac
     1921 ttgctttgat tgcattgtga tatactgcta acatccaacc gtcataagtt ttttccgtga
     1981 tcccgtctgc ttggtaggtt acaagagagt ttaccccgta gcacatccaa gggctattta
     2041 acacttttgc attagatagt ctagttgcat aagcgtctgt attagcagca cccgcaccac
     2101 caaaggtttc attacttcct tctggagaaa ccattttaga gcaatgatca gccaaataag
     2161 tagccactga aaaggtagat gtaagaacgt ttctataaaa accagtaacc gattcgcata
     2221 ag
//
LOCUS       DAOQOS010000276         2104 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_47400_length_2104_cov_5.532455, whole genome shotgun sequence.
ACCESSION   DAOQOS010000276 DAOQOS010000000
VERSION     DAOQOS010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 2104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2104
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47400_length_2104_cov_5.532455"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            314..916
                     /locus_tag="PLF66_26515"
     CDS             314..916
                     /locus_tag="PLF66_26515"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HMZ67668.1"
                     /translation="MDFLEGYLVGLGMIVFIGPVFFLLLSVSLQLGGTAGSLAAFGIF
                     VSDIVCVCLCYYGVTSFLNDPTTKNWIVLSGGLLLILLGSKYMFKKDFKIEEKKDIAS
                     LRYISCFVKGFLVNFVNPFVFFVWIGVITYEKTLEANGCNPLIYLSGTLLAILTTDLS
                     KVFLAKWIRKFLNPRKLGYFYKASGIFLIGFGIRLLLLFN"
     gene            1034..1534
                     /locus_tag="PLF66_26520"
     CDS             1034..1534
                     /locus_tag="PLF66_26520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014802889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1761 domain-containing protein"
                     /protein_id="HMZ67669.1"
                     /translation="MKLNFLILSLSALVPLVVGFIWYHPKVMGNAWMAASGMEEDKTK
                     GISLPLILFLSYIFSLFLALAIQAFVIHQNHVYSILINEPGFREPGSEIQNFITLFMD
                     KYGRNFRTFKHGAFHGTLAGIFFVLPIVATNALFERKSAKYIAIHTGYWTICTTLMGG
                     IICQFA"
     gene            complement(1515..>2104)
                     /locus_tag="PLF66_26525"
     CDS             complement(1515..>2104)
                     /locus_tag="PLF66_26525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HMZ67670.1"
                     /translation="NDYMDAMVTKFRTKARTAIRKSEELTIRNLTLSEIDSLKNKIES
                     LYLQVQEKSSFWFGALNANSFLYLKQNLGEDFILKGYFLGNELIGFSSSFLFDSILDA
                     NYVGINYELNRDFAIYQRMLYDYIDLAIERGCKELRFGRTAEEIKSSVGAVPVPMKLF
                     IRHKNPIANRILKAIVATIHPSSYELRKPFKQTDK"
BASE COUNT          649 a          332 c          370 g          753 t
ORIGIN      
        1 cttattccac ccttcgctcc aagtattcac gatcagcttc gcatgtgcgc ttcgtagtaa
       61 aaaaaaaact gatagaaaaa gatatttcac cacgaaggac acgaaggaac acgaagttag
      121 gttagaaata aaaaggaaac ccttcgtact tattccaccc tttgctccaa gtattcccga
      181 ccagcgcctt cgcatgtgca ctttgtggtc aaaaaaacaa agagtaaaag atgattctag
      241 ttcaatacac aaggtgaatt acggacttgt attttttgaa tgagcaaagc atcttggaag
      301 gaaaggaaat agaatggatt ttttagaggg ttatttagtc ggactaggaa tgattgtatt
      361 tattggacct gtgttttttt tattgttatc cgttagtcta caactgggtg ggactgctgg
      421 ttctctcgcc gcatttggaa tttttgtaag tgatatagtt tgtgtttgtc tttgttatta
      481 tggtgttact tcttttttaa atgacccaac tactaaaaat tggattgttc ttagcggggg
      541 cttactactg atactgcttg gaagtaaata tatgtttaaa aaggatttta aaatagaaga
      601 aaaaaaagac atagcttctc tccgttatat ttcttgtttt gtaaaaggtt ttctggtaaa
      661 ctttgtaaat ccatttgtat tttttgtttg gattggtgta ataacctatg aaaagacatt
      721 agaggcaaat ggatgtaatc ctctcattta tctttcagga acacttttag caatacttac
      781 aactgattta tcaaaggtat ttttagcaaa atggatccga aaatttttaa atccaagaaa
      841 gcttggttat ttttataaag cttctgggat cttcttgatt ggatttggga ttcggttgtt
      901 attgctcttc aattgaagat tgatcctata aaaatagcat tggaaagtta aaagtttaaa
      961 tgaccatttt attttcctgt ttaagaaaac ataattattt gacagaatca aatcaaaatt
     1021 ctaaacaaac tctatgaaac taaatttctt aattttaagt ctttcggctc ttgttccgct
     1081 tgttgtcggt tttatttggt atcacccgaa ggtgatggga aatgcatgga tggctgcctc
     1141 tggtatggaa gaagacaaaa caaaggggat aagtttacct ctaatcttat ttctatctta
     1201 tatattcagt ttatttcttg cacttgcgat tcaggcattt gtgattcatc aaaatcatgt
     1261 ttattctatc ttgatcaatg agccaggatt tagagaacct ggatcagaaa ttcaaaattt
     1321 tataactttg tttatggata aatacggaag aaatttcagg acattcaaac atggagcttt
     1381 tcatggaact ttagctggta tcttttttgt gttgcctatt gtcgctacta acgcattatt
     1441 tgaaagaaaa tctgctaaat acattgcgat tcatacaggc tactggacta tctgtactac
     1501 tctcatgggt gggatcattt gtcagtttgc ttaaacggct ttctgagttc gtatgaactt
     1561 ggatgaattg tcgcaacgat tgcttttaag atacgatttg caattggatt tttatggcga
     1621 ataaaaagct tcattggaac aggaactgct ccgactgagc tttttatttc ctctgccgtt
     1681 ctaccaaaac gtaattcttt acaacctctt tctattgcga ggtcaatata atcgtatagc
     1741 attctttggt agattgcaaa gtctcgattt aattcatagt ttatacccac ataattagca
     1801 tctaaaatag aatcaaagag aaaggaagaa ctaaatccaa tgagttcatt tcctaaaaag
     1861 tatcccttta aaataaaatc ttctcctaga ttttgtttta aatacaaaaa tgaattcgca
     1921 ttcaatgccc caaaccaaaa agaagatttt tcttgaactt gtaaatatag actttcaatt
     1981 ttatttttga gtgaatcaat ttcagataat gttaaatttc gaatcgttag ttcctctgat
     2041 ttgcgaatgg cagtccttgc ttttgttcta aattttgtta ccattgcatc catataatca
     2101 ttta
//
LOCUS       DAOQOS010000277         1880 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Leptospiraceae bacterium isolate AMR_MDS_0464
            NODE_58706_length_1880_cov_4.001096, whole genome shotgun sequence.
ACCESSION   DAOQOS010000277 DAOQOS010000000
VERSION     DAOQOS010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32810096
            Sequence Read Archive: SRR3501853
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Leptospiraceae bacterium (wastewater metagenome)
  ORGANISM  Leptospiraceae bacterium
            Bacteria; Pseudomonadati; Spirochaetota; Leptospiria;
            Leptospirales; Leptospiraceae.
REFERENCE   1  (bases 1 to 1880)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1880)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 07:22:45
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,306
            CDSs (total)                      :: 5,267
            Genes (coding)                    :: 5,232
            CDSs (with protein)               :: 5,232
            Genes (RNA)                       :: 39
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 36
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 1 of 35
            Pseudo Genes (incomplete)         :: 30 of 35
            Pseudo Genes (internal stop)      :: 7 of 35
            Pseudo Genes (multiple problems)  :: 3 of 35
            CRISPR Arrays                     :: 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1880
                     /organism="Leptospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58706_length_1880_cov_4.001096"
                     /isolate="AMR_MDS_0464"
                     /isolation_source="Domestic waste water"
                     /db_xref="taxon:2026757"
                     /environmental_sample
                     /geo_loc_name="Singapore"
                     /lat_lon="1.33 N 103.75 E"
                     /collection_date="2015-06-12"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1318)
                     /locus_tag="PLF66_26530"
     CDS             complement(<1..1318)
                     /locus_tag="PLF66_26530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020762501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas3''"
                     /protein_id="HMZ67671.1"
                     /translation="MKKDEFLAHVKKEENGEWKLHLLEEHLREVAKLSGQFSSKFGAE
                     SFGYLIGLWHDLGKYKQDFQERIKLKSGYDEEAHLEGKTAKSVKHAASGAIHCLNKFK
                     QSELLKKFIALPILCHHSGLKNYGVDVDIQISEEKEINLLSNTISQIPAEILEGSKLK
                     PLTQSFLDESLFIRMLFSALIDSDRLDTEKFMNPEKFQERYSIHISLEELNQKLDLHL
                     EDKQKNSEPTKVNLLRKRILQEVESKAKLTSGFYTLTVPTGGGKTLTSLSFALKHCLE
                     NQKDRIIYAVPYLSIIEQTAKIFKDILGEDSVLEHHSSIDISKEKENFRNRLLAENWN
                     HPLIVTTNVQLFESLFSARTTKIRKLHNLSNSVIILDEAQMIPPDFLQPIVKALKELV
                     KCYNVTIILCTATQPTLSSINSMNFKFDGIDNAIELAPTPEELFQDL"
BASE COUNT          577 a          348 c          283 g          672 t
ORIGIN      
        1 ttaaatcttg gaatagttct tctggagtcg gagcaagctc tattgcattg tcaataccat
       61 caaatttgaa attcattgaa tttatagacg ataatgtcgg ttgggtagcc gtgcaaagaa
      121 taatggttac attataacat ttcactagtt ctttaagtgc ttttacaata ggctgtaaaa
      181 aatcaggcgg tatcatttga gcttcatcta gaatgataac actattcgat agattatgca
      241 gttttctaat tttagtagtt cttgcagaaa ataaactttc aaataattgc acattcgttg
      301 ttactatgag cggatgattc caattttccg ctaataatct gtttcggaaa ttttctttct
      361 ctttggaaat atctatacta gaatgatgct ctaaaacaga atcctctcct agaatatctt
      421 taaatatttt tgctgtttgc tctatgatac ttaaataagg aaccgcatag atgattcgat
      481 ctttttgatt ctccaaacaa tgtttgagtg caaaggaaag cgaagtaaga gtttttcctc
      541 caccagtcgg aacggttaac gtatagaaac cggacgttaa ctttgctttt gattcaactt
      601 cttgtaagat tctttttcta agtagattta cttttgtggg ttcggaattt ttttgtttat
      661 cttctaaatg caaatccaat ttttgattca attcttctag actgatatgt atagaatatc
      721 tctcctgaaa cttttcagga ttcataaact tttctgtatc gagtcgatcc gaatcaatta
      781 aagcagagaa tagcattcga atgaataaag attcatcaag gaaggattga gttagaggtt
      841 tcagtttact accctctaaa atttctgccg gtatctgtga aatggtattg gataataaat
      901 taatttcttt ttcttcggaa atttgaatat caacatcaac gccgtaattt tttaaacctg
      961 agtgatgaca taaaatggga agagcgataa attttttaag gagttcactt tgcttaaact
     1021 tatttaaaca atgtatcgcc ccgcttgcag catgttttac tgacttcgca gtctttcctt
     1081 ccaaatgagc ttcttcatca tatccagatt taagctttat tctttcttga aaatcctgct
     1141 tatacttccc taaatcatgc caaagaccaa tcaaatatcc gaaactttct gcgccaaatt
     1201 tagaactaaa ttgtccagat agttttgcca cttcacgtaa atgctcctcc aaaagatgaa
     1261 gtttccattc tccgttttcc tccttcttca cgtgtgctaa aaattcatct ttcttcatgt
     1321 gcataatatc cattatccta tttgcgggat cgcatgtatg cgatccctac gaatttggat
     1381 ctgtcaaata cagaatatat cagtgcagct ccaacgcctg gccttgtcca cataaccgtc
     1441 gaaccttcta tttcaaattc tacattatat ttcttcattc gacgttttcc tcttttcgac
     1501 acatgcgcta tatagctcat aatccttctt ccagaatcct attttgacca ttataaatgg
     1561 atagccggac aaaatagaat ggcgcattaa taaaagttga ttttttactc aaatattctt
     1621 gagtcataat atggaatatg tcaataagaa tcaaatattt ctatgatact agaggatttt
     1681 atcatataac ttgatttaga attaaatttt aagatgggtc agaaaaaaga ctcactgaac
     1741 gtaaaagtat tttaaaatta caattataaa tccatatctt tcaattgctt gagaatacag
     1801 aaacctgcat tacgatccaa catctccctt tcttcgtatt accttcgtat taacgataca
     1861 ctatctggct actatctcgc
//
